LOCUS       DASVDU010000001         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1736217, whole genome shotgun sequence.
ACCESSION   DASVDU010000001 DASVDU010000000
VERSION     DASVDU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736217"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF226_00005"
     CDS             complement(<1..515)
                     /locus_tag="VF226_00005"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072401.1"
                     /translation="MSAAPFLASLLLGGPAAAALVAAVGSTDLREIRRQVPWYGTVFN
                     HASVVLSVVMGGAVFELIRREVADLPAAAALALTFAGLLIGSGIYYMINAILALMAVS
                     ARTGTPIRTIWAQDLGAVMATLLGLAPLAWLMAEIFRLPNGVGWWATPLFVVPLFTTR
                     LAYHRYVETREL"
     regulatory      complement(954..1036)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(1125..>3821)
                     /locus_tag="VF226_00010"
     CDS             complement(1125..>3821)
                     /locus_tag="VF226_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072402.1"
                     /translation="ELAELERRTEGWAASLQLVEVSLRERHTAEERRAFIQSITATRD
                     SDLFSFLAEEVLDQQEPSVQDFLLVTSILAQVHPELAERLSGSMDGARMLTTLEHRGL
                     FTYRLDTAEDLYRYHGLFRDFLQRRLSADRSEGEIAGLHIHAASFYETHQLWPEAIHH
                     YLRARLQPQAARLIARFGEEVASEGRLGLIDEWLQEISPRVIRDNARLSLLFGEARGI
                     GGDWHAALSALGRARAFFARKGDRRMEALACLKLSTVYANHGDIALAIEAASNGIDLA
                     PEDAAATRLRLSGNLAITSTWMEAPLEDVAKVCQRITVEAKARGWEHFAAIALHNLGA
                     AQRNMGRIGDSIKSLELAAHFWATSPASPFADNAELVASLLCADQLTRASAIAEAAIV
                     RTRPWSRPHAEAQYGKLLVLSYQGRHEEAVEVAQRLLQDPDPLGPTADLVMAHLVESQ
                     WLLSQAGSVQLSSAAPSGTLDPRLAPLIAPAQAIASHRASPCAGECLAAIEGLESWDM
                     RGATLTATVGLVKLGRLALEHTSRPNTRRVITSVMRAAEAGILRMLRPWIRLYAPFAR
                     SILREPGGADALLLMAEADPEAWRDAVISLLAGRGMSDRARLLQLVTRIASKDTAAAL
                     RDIEGADVAAVRRLLVHRHAPRLYVRSFGSMSLHHGAWDGPSATIERRRMRNLLGLLI
                     AYDGSTLTRDVALDILWPDADPGAAINSLNQTVFQLRRTIDPDYRDGESAQYIISTTD
                     VVQLNPELVRTDLREFRALAALVRSDQVSDSRSAGARMIDLVRGEFLAELKYEDWPSH
                     IRTAVHSEVRDALLPLANGVHGFTSDLSVRAACALVELDEFDEQAYVAMAEQLAAAGK
                     RAAARDVLIRFAKRLEAELSEPVPESIATVLATLPQVSPPVQ"
BASE COUNT          755 a         1185 c         1208 g          673 t
ORIGIN      
        1 agctcgcgcg tctccacgta gcggtggtac gccagccggg tggtgaagag cgggacgacg
       61 aagaggggag tcgcccacca gccgacgccg ttggggagcc tgaagatctc cgccatgagc
      121 caggcgagcg gcgccaatcc caagagcgtt gccatgacgg cgccgagatc ttgggcccag
      181 atcgtccgga tcggcgtccc ggttctggca ctgacggcca tcagagcaag gatcgcgttg
      241 atcatgtagt agattccgct tccgatcagc agaccggcaa acgtgagagc aagcgccgct
      301 gctgccggca ggtcggcgac ctcccgccgg atcagttcga agacggcgcc ccccatcacg
      361 actgaaagca cgacagacgc gtgattgaag accgtcccgt accaaggaac ctgccgtcgg
      421 atttcgcgga gatcggtgct gccaactgct gcgactaagg cggccgccgc agggccgccg
      481 aggagcaggc tcgccaggaa aggcgcagcg ctcaccgata cgactgttcc atgcggaagc
      541 cgcacgggga acagcgagcc tatgagagtg agcccaatcc agaacgccaa acctattggc
      601 ccctggattt cagccctaac tgggaatagg aaagccgccg ccacgacagc aacggtcgca
      661 gcggcggaac atgcgagtat caggcgccgg acattgtcat tcaccgaact tcaccctagc
      721 ggggtgaggt cggcgagtct accatccgga cgcctaccaa cccttgaagc tggcgccccc
      781 agcaatcgct aaggtaatca gcgtgccaag ggaggtcagc aacgccatga ggcgtcgtgg
      841 aaacgcacgc atgcgtgact ccttggactg attaccagcc cttaatgcat tcgcgagcca
      901 tcagaaggtg ccctcttctg aacgtattta ccaagacaac cgacactttc cggtgacgga
      961 acggtcggtt gcactacatg ctccccgacg gcccaagtgc ccacattcgg ccggcggatc
     1021 gaagcaccct cggtattgcg ccgtggtacg aaagtaccat ggctcgccgg tactgtacgc
     1081 tcgcccacgg ggtaagtcaa cccccgatgc gaaacggtca ataactactg aacgggaggc
     1141 gacacctgcg gaagtgtagc caacaccgtg gcgatcgatt cagggaccgg ttccgacagt
     1201 tcggcctcta gccgcttcgc gaaacgaatc agaacgtcac gcgccgcagc tcgcttgccg
     1261 gctgcggcca gctgttccgc catggcgacg tacgcttgct catcgaattc gtcgagctcc
     1321 accagtgcac aagcggcgcg gacgctaagg tcgctcgtga acccgtggac gccgttggcc
     1381 agcggcagca gcgcatctct gacttctgag tggactgccg tacgtatgtg cgacggccag
     1441 tcctcgtact tgagctcggc caggaactcc cctcggacca gatcgatcat gcgggcgccc
     1501 gcacttcgag aatcggatac ctgatcgctc cgcacgagag cggccaacgc acgaaactct
     1561 cgaagatcgg tacggacaag ctcgggattc agctggacga catccgtggt gctgatgatg
     1621 tactgcgcgc tctctccgtc tcggtaatcc gggtcgatcg tgcggcgcaa ctggaagacc
     1681 gtttgattga ggctgttgat agccgctccg gggtcggcat ccggccaaag gatgtcgagt
     1741 gccacgtccc gagtcagcgt gctcccgtcg taggcgatca ggaggcctaa caagttgcgc
     1801 atccgccggc gctcgatcgt cgcactgggt ccatcccacg cgccatgatg gagggacatc
     1861 gatccgaaag aacgcacgta gagccgaggg gcgtgacggt gaacaagcaa gcggcgtacg
     1921 gccgcaacgt ccgcaccttc gatgtcccgc aaggcggcag cggtgtcttt ggaggcgatc
     1981 ctggtcacga gctgaagaag ccgggctcgg tcgctcatcc cgcgtccggc aaggaggctg
     2041 atcaccgcgt cccgccacgc ctctggatct gcctctgcca tgagcaacag ggcatcggca
     2101 ccgcctggct cgcggagaat gcttcgggcg aagggggcgt agaggcgaat ccacgggcgg
     2161 agcatcctca gtattccggc ttcagcagct cgcatgaccg aggtgatcac ccttcgagta
     2221 ttcggtctgc tggtgtgttc cagagccaag cgccccagtt tcactagccc tacggtcgcc
     2281 gtcagtgtcg cccctcgcat atcccaggac tcaagtccct cgatggcagc taggcactca
     2341 cccgcacatg gagacgcgcg atggctggct atcgcctgag cgggcgcgat caacggcgca
     2401 aggcgggggt cgagtgttcc actcggcgcg gcgctcgata actgcacgga accggcctgt
     2461 gaaagcagcc actggctctc aacaagatgc gccatcacaa ggtcggcggt tggcccaagg
     2521 gggtccggat cctggaggag gcgctgagct acctcaacag cctcttcgtg ccgcccttga
     2581 taggaaagaa cgaggagttt tccgtactgt gcttcggcat gtggccgtga ccacgggcgg
     2641 gttcggacga ttgcagcctc cgcaattgca gatgcccgtg tgagctgatc tgcacatagc
     2701 agcgaagcca cgagttcggc gttgtccgcg aatgggcttg ccggagacgt agcccagaag
     2761 tgggcggcga gttcgaggct cttgatgctg tcaccaatac gacccatgtt gcgctgagcg
     2821 gcgcccaggt tatgcagggc aatcgcggcg aagtgctccc accctcgcgc ctttgcttcg
     2881 acggtaatcc gctggcatac cttggctacg tcttcgaggg gcgcttccat ccacgtggac
     2941 gtgatggcga ggttgccact gagccgcagt cgcgttgcgg ctgcgtcctc cggcgccaag
     3001 tcaatcccgt tcgacgcggc ttctatcgcg agcgcgatgt ccccgtggtt tgcatagacg
     3061 gtgctgagct ttaggcacgc gagtgcctcc atccggcggt cacctttgcg agcgaagaac
     3121 gctcgggctc gaccaagggc actgagcgcg gcatgccagt ccccgccgat tccccttgcc
     3181 tcgccgaaca gaagggatag gcgagcgttg tcgcggatga cgcgcggtga gatctcttgc
     3241 agccattcgt cgatcagacc gagccttccc tccgaagcga cctcttcgcc aaagcgggca
     3301 atgaggcgcg ctgcctgtgg ttggagacga gctcgcaggt agtgatgaat cgcctcgggc
     3361 cagagctggt gagtctcgta gaacgacgct gcatgaatat gcagtcccgc gatctcgcct
     3421 tcagatctat cggcggaaag tcgccgttgg aggaaatctc tgaagagacc gtggtagcgg
     3481 tagaggtctt cagctgtatc gaggcgatag gtgaagagac cgcgatgctc caacgtggtg
     3541 agcatccgag caccatccat gcttccggag agccgctctg cgagttcggg atggacttgc
     3601 gccaagattg aagtgacgag caagaagtcc tgcacagaag gctcttgctg atcaagtact
     3661 tcctcggcga ggaacgagaa gaggtcgctg tcgcgggtgg cggtgatgga ctggatgaac
     3721 gcccgccgct cctcggccgt gtggcgctcg cgcagcgaaa cctccacgag ctgcaggctc
     3781 gcggcccagc cctcggttcg gcgctccagc tcggccagct c
//
LOCUS       DASVDU010000002        11360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_188165, whole genome shotgun sequence.
ACCESSION   DASVDU010000002 DASVDU010000000
VERSION     DASVDU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188165"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF226_00015"
     CDS             <1..429
                     /locus_tag="VF226_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX7072403.1"
                     /translation="FPEATPRTMRVERTFWEKATAVHVYCLQGRARGDRFARHWYDLV
                     RLDEAGHADAALKDHDLALAVARHKSMFFAEKDAEGASIDYEAAVRGGLVLVPEGAAF
                     EALAKDYARMVEDGLLLNDPDRFEDLMESCRQIAKKANAV"
     gene            complement(468..1898)
                     /gene="merA"
                     /locus_tag="VF226_00020"
     CDS             complement(468..1898)
                     /gene="merA"
                     /locus_tag="VF226_00020"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HEX7072404.1"
                     /translation="MADCCTPAKSSKATYDLAVIGAGSAGFSAAITAAELGANVALIG
                     HGTIGGTCVNVGCVPSKTMIRAAEALHGARAAGRFPGLAGEAQVNDWRHLIAAKDELV
                     STLRQKKYADLLPEYNTIAYLEGEARLNGAGVIVDGTAIAADKTLIATGGRPAVPPIP
                     GIDKVKYLTSTTLLELEELPKSLIVIGGGYIGAELAQMMARMGVKVTVICRSRLLPQA
                     EPEVSAALADVFQAEGITLHCGIAYHACHEDETGVTVCVKEGGRLIELTAERLLVATG
                     RTPNTEALGLADAGIKQDSRGAVVVDDRMRTSKAGVYAAGDVTDRDQFVYMAAYGAKL
                     AALNALNGDSLSYDNSAMPWVVFTDPQVAGVGLSEAAAKAAGYAVKTSVLPLDSVPHA
                     LAARDTRGLIKLVADAKTDRLLGGQILAPEGADSIQTLAMALKFGMTTKALGATIFPY
                     LTTVEGLKLAAQTFDKDVAKLSCCAG"
     gene            complement(1900..2148)
                     /gene="merF"
                     /locus_tag="VF226_00025"
     CDS             complement(1900..2148)
                     /gene="merF"
                     /locus_tag="VF226_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system transport protein
                     MerF"
                     /protein_id="HEX7072405.1"
                     /translation="MKDATILKTGIIGAVIAAICCATPVLVIAFGALGLSAWVSGLDY
                     VLLPALAVFVGLTAYGLWRRNRAAACCDANIETNKQRA"
     gene            complement(2154..2486)
                     /locus_tag="VF226_00030"
     CDS             complement(2154..2486)
                     /locus_tag="VF226_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEX7072406.1"
                     /translation="MKRPLILAAALLTLGGLGWAIIPAVTPAAAQSASQQVTAAQTRT
                     FAIQNMTCALCPVTVKKAMEGVSGVKSVAVDFEAKTATVIFDPAVTNADAIAAASTNA
                     GYPASVKG"
     gene            complement(2510..2908)
                     /locus_tag="VF226_00035"
     CDS             complement(2510..2908)
                     /locus_tag="VF226_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transporter MerT family protein"
                     /protein_id="HEX7072407.1"
                     /translation="MSTTETETRPLQAVGDTEERKRNLFAAGGVIGAILASTCCVVPL
                     LFVTLGISGAWIGNLTALEPYKPYFAGVALVFIGLGFRQVYFKAKPACVEGSYCARPQ
                     SAVITKTALWLSTVIVLLALTINWWAPLFY"
     gene            2987..3400
                     /locus_tag="VF226_00040"
     CDS             2987..3400
                     /locus_tag="VF226_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7072408.1"
                     /translation="MDDHVSGKGLRRADLARRTGCNLETIRYYEKIGMMPEPPRTASG
                     YRVYDERHVARLRFVLRGRELGFSIEELRGLLRLVDRGTQTCAEVKAMTEQHLADVRA
                     KIADLRRIEKVLAATAAQCSGEEVPECPVLEALAS"
     gene            3400..3726
                     /locus_tag="VF226_00045"
     CDS             3400..3726
                     /locus_tag="VF226_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HEX7072409.1"
                     /translation="MATVAVYIGAAVAEIAGCFAFWAHFRLGRSIFWLAPGLVSLVLF
                     AFLLTRVDSDFAGRAYAAYGGIYIAASILWLWLVESRTPDRWDLVGAAICLLGAAVIL
                     WGPRGT"
     gene            3832..4812
                     /locus_tag="VF226_00050"
     CDS             3832..4812
                     /locus_tag="VF226_00050"
                     /inference="COORDINATES: protein motif:HMM:NF016670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HEX7072410.1"
                     /translation="MKSGQFAPLPLEEIEPISCSAVSGGAAASSGAVFPANDNHPVPR
                     QLSKLFTSALAIELEEAREAGALGFMARAMVQATMPHRRMPGNEFTRTNGAFTMTMLA
                     PSKIGLPYGSIPRLVTGFLTTEAVRTKSREIVLGRTLSKFMAELDLAPTGGRWGTIPR
                     LRDQMTRLFACSISCTYTSGNSFGLENVNIADKATLWWTPHQPDQAALWESTVVLSDR
                     FFTEITENPVPIDLRALKALKRSPMALDVYLWLTYRLSYVKGRTAIPWAALQLQFGAD
                     YPTTDQGRRDFKKAFLRALGKVAVVYPAARLDTAPDSLILLPSRPHIGRS"
     gene            complement(5152..5325)
                     /locus_tag="VF226_00055"
     CDS             complement(5152..5325)
                     /locus_tag="VF226_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072411.1"
                     /translation="MMENLITIAVVVVVGIVVLNLVFWNKNKKAFNDAKEQKIAERQA
                     QREQAEEHRRQKK"
     gene            complement(5404..7422)
                     /locus_tag="VF226_00060"
     CDS             complement(5404..7422)
                     /locus_tag="VF226_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX7072412.1"
                     /translation="MVFGSLFGGGKKPPEGKPQVRRWLESLDPMWRAAVTAIAADKSV
                     DEVFKQDIYRRLQEGRIDDAREAVRKYFQFMSPETRERVKHAGQHEAWADSDALREAG
                     LITPTSESDPVDSIIIGELPLGDDLRPANLLFHGEGHLLTVAPTGAGKGQRFVVPTLL
                     DYEGPAVIFDPKGENYELTAWRRNLYGQVYKWEPKSEDATIDPESDCYNPLDAVKGWD
                     DARLLAELLIVQQSREPFWDEAAKDLLTGLIFYVLRTRPPERRNMREVCRCLTLSKAD
                     FTGMIESLQGSSEERLQELGNILESRSENLHSSIATTLLTQLAPWRADEIADITSTTT
                     PGWSVESILVQDNMGVTFAAQQGRAPGWHSPAPGHVERGMAASVYLIVKPEKIAAYRS
                     VLRVMLGQHLGEAIKWRADIERNNRLDEDLPERFPDWPMMFYLDELPQLGYMRIIEEA
                     VAITRSYRVRLWLFTQDMAQLKEVYPKWESLLANCRCQIFFRPNDMNTATEIANRLGM
                     RKDIWGGEDWVASPQKLMGREFREDAVIFLDGLMIRSRMVKPAHGNPELKAWIAEQKR
                     DFGEEVLRAPRAEAPIPAPDDEPDAPPSGSAESAAPTKSEGAQKEDANLAGNPEYQRR
                     LAALQAEMRAQKPEANPPSTENGPRPPERPVGPSEEAKRPPMPPSFEE"
     gene            7946..8854
                     /locus_tag="VF226_00065"
     CDS             7946..8854
                     /locus_tag="VF226_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEX7072413.1"
                     /translation="MTAQRIDVYTQVTNNIIAAIEAGAGDWQMPWHRSGDGLNRPVNI
                     DTNNAYRGINVVSLWAAGQARGFSTGTWGTYRQWQNNGAQVRKGEKASLVVFYKEFDV
                     EEATDDGETEQGKRLMARASWVFNADQVDGYEAPALPEPKDPVQVLDKAERFIADTGA
                     IVHHGGSRAFYRPSDDIIQMPERERFLGTETSTATESYYATLLHELTHWTGHDRRCDR
                     RFGKRFGDDAYAMEELVAELGAAFLCADLGVTLTPRPDHAAYIDNWLKVLKADKKAIF
                     TAASQAAKATDYLGGLQSADITEAAA"
     gene            complement(8868..9455)
                     /locus_tag="VF226_00070"
     CDS             complement(8868..9455)
                     /locus_tag="VF226_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072414.1"
                     /translation="MALSVTPIHRAFAQAASSNSGGILDGLLDALVVLLVVVAALFLR
                     KKLRNRAQRHGPFFAEIRASPEGMQKLRAQMKSARAIAIVLAVPAVLVTVVLLIKINN
                     TSNWALRYDGYVWEQLGWWFIGVGLLWLLTLVAGGFWFVTARNFGRIVLDRVATIRTR
                     LVEIEKELETRADRPKADLRSERQLLESELRQLGA"
     gene            complement(9494..9880)
                     /locus_tag="VF226_00075"
     CDS             complement(9494..9880)
                     /locus_tag="VF226_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072415.1"
                     /translation="MRNTYLSKGWLIAIALLTVTACQTTGATSAKSADADPLAAYKAA
                     VPALEQAGGGKACVDCFLTNYGPSPFPKAFAVSPDGAYGARYKVGASMAKMREDALAS
                     CRKKPAYNPAHDCFIFFENDQQVWKP"
     gene            complement(9891..10214)
                     /locus_tag="VF226_00080"
     CDS             complement(9891..10214)
                     /locus_tag="VF226_00080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7072416.1"
                     /translation="MNTKDKVVARSASRRDVLRALGVAGLATVGGTIASQPAMAEVNL
                     PATATQKCGTCRFWGGTRSVSEDRKTVIASGKGTCNNPKSPAYQKQTRPDQGAPVWER
                     WEALG"
     gene            10463..11272
                     /locus_tag="VF226_00085"
     CDS             10463..11272
                     /locus_tag="VF226_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072417.1"
                     /translation="MSGAFDSFGEWHRLFDALCIERGHLDNATLASEYCALTKNKAST
                     AYESSLKSLNNWRQGLHTPSRRNFRILTLILRVEDAPEVLPHWSRLYEEAQRRKPADG
                     NGDASQTATIPEIDPAPTAASGGRASRRLAAAAVLLFLGAGGAVLALGTFDGPQTHAA
                     AFTQPGAQVLDMTGQRIEAHEMVTAKVGQSVVVHGSRGRCGEQPPDWEEVFQGLPPLS
                     NGVWSDGGVGIRVSRACSGGTPARAVVFTATHPGEERFTLYDDPITIRVTE"
BASE COUNT         1949 a         3692 c         3569 g         2150 t
ORIGIN      
        1 ttccccgaag ccacgccgcg cacgatgcgt gtcgagcgca ccttttggga aaaggcgacg
       61 gccgtgcatg tctattgcct gcaaggccgc gcccgtgggg accgcttcgc ccgtcactgg
      121 tacgatctcg tccgcctgga cgaggccggt catgccgacg ctgcgctgaa ggaccacgac
      181 ctggccctcg cggtcgcgcg gcataagagc atgttcttcg ccgagaagga tgccgagggg
      241 gcctccatcg actatgaggc ggccgttcgc ggcggacttg tcctggtccc cgaaggcgcg
      301 gcgttcgagg cgcttgccaa ggactatgcc cgcatggtcg aggacggctt gctgctgaac
      361 gatccggacc gcttcgagga tctgatggag agctgtcggc agattgccaa gaaggccaac
      421 gccgtctgac ccacgagaac cgcgcaggca taccgggtgt tggcgattca accggcacag
      481 caactcagct tcgccacgtc cttgtcgaag gtttgggcag cgagcttcag cccctctacc
      541 gtggtgaggt agggaaaaat cgtcgcgcca agcgctttcg tggtcatgcc gaacttcagc
      601 gccattgcca gtgtctggat gctgtccgcg ccctccggcg cgagaatctg gccaccaagc
      661 aggcggtcgg tcttggcgtc ggccaccagc ttgatcagcc cccgcgtgtc gcgggcagcc
      721 aaggcgtgtg gcacgctgtc gagcggcaga acggaggtct tgaccgcgta accggcggcc
      781 ttcgccgccg cctcgctgag gcccacgcca gccacctgcg ggtccgtgaa cacgacccac
      841 ggcatggcgc tgttgtcgta ggaaaggctg tcgccgttga gcgcgttgag ggcggcgagt
      901 ttggccccat aggccgccat gtagacgaat tggtcgcggt cggtcacatc gccggcggca
      961 tagacgccgg ccttggatgt gcgcatccgg tcatcgacga cgacggcgcc gcggctgtcc
     1021 tgtttgatac cggcgtcggc gaggcccagc gcttcggtat tgggcgtgcg gcccgtcgcg
     1081 accagaagcc gttcggccgt cagctcgatc agcctgccgc cctccttgac gcagaccgtc
     1141 acgccggttt cgtcttcgtg gcaggcatgg taggcgatgc cgcaatgaag ggtgatgccc
     1201 tcggcctgga acacatccgc caaggctgcg gagacttccg gctccgcctg cggcagcagc
     1261 cgcgagcggc agatcaccgt caccttcaca cccatgcgcg ccatcatctg tgcaagctcg
     1321 gccccgatat agccgccgcc gatgacaatc aggctcttgg gtagctcttc caactccagc
     1381 agcgtcgtgc tggtcaggta cttcaccttg tcgatgccgg gaatgggcgg tacggcgggc
     1441 cgtccgccgg tggcgatgag ggttttatcc gcagcgatgg cggtgccgtc cacgatgaca
     1501 ccggcgccgt tcagccgggc ctcaccttcc agataggcga tggtgttgta ttcgggcagg
     1561 aggtccgcgt acttcttctg cctgagcgtc gagacgagtt catccttggc ggcgatcaga
     1621 tgccgccagt catttacctg cgcttctccg gccagacccg ggaagcggcc cgccgcccgc
     1681 gcgccgtgaa gcgcctcggc ggcccggatc atggtcttgg agggtacgca gccgacattg
     1741 acgcaggtgc cgccaatggt gccatggccg atgagcgcca cgttcgcgcc cagttcggcg
     1801 gcggtgatgg cagcggaaaa ccccgccgag ccggcgccaa tgacggcgag gtcgtaggtc
     1861 gctttggatg atttggccgg ggtgcagcag tccgccatgt caggctcttt gcttgtttgt
     1921 ctcaatgttc gcgtcgcaac aggccgctgc gcggttgcgc cgccacaggc cataggccgt
     1981 cagaccgacg aagacggcga gcgcgggcag aagcacgtag tcgagcccgc tcacccaggc
     2041 cgacaggccg agcgccccga aggcaatgac cagcacgggg gttgcgcagc agatggcggc
     2101 gatgaccgcg cctatgatgc cggtcttcag gatcgtggcg tctttcatct ggctcagcct
     2161 ttgaccgagg ccggatagcc cgcattggtg gaggcggcgg caatggcgtc cgcgttcgtc
     2221 accgccggat cgaagatgac cgtcgccgtc ttggcctcga aatcgacggc caccgatttc
     2281 acgcccgaaa caccctccat ggccttcttc accgtgacgg ggcacaaggc gcaggtcatg
     2341 ttctggatgg cgaaggtccg ggtctgggcg gccgtcacct gttgggaagc ggactgggcg
     2401 gcggccgggg tcacggcggg tatgattgcc cagccgagtc caccgagggt cagcaaggcg
     2461 gcggcgagga tgagaggtcg tttcatggcg gtaactcctt tgtttggtct cagtagaaga
     2521 gcggcgccca ccagttgatg gtcagcgcga gcaggacgat gacggtggag agccaaagcg
     2581 cagtcttggt gatgacggcc gactgcggcc tggcgcaata gctgccctcc acgcaggcgg
     2641 gcttggcctt gaagtagacc tgccggaagc cgaggccgat gaagaccaga gcaacgcctg
     2701 caaagtacgg cttgtagggc tccagcgccg tcaggttgcc gatccacgcc ccggaaatcc
     2761 cgagtgttac gaacagcagc ggcaccacgc agcaggtcga ggccagaatg gccccgatca
     2821 cgccgcccgc cgcaaagaga ttccgcttcc gttcctccgt atcgcccacg gcctgtaacg
     2881 gccgtgtttc tgtttcggtt gtactcatgc gtcatcatcc tcgcttccgt tctgaattta
     2941 agataaggtc tgtagcaact acagactcaa ggggcttttc gagcacatgg acgatcacgt
     3001 ttccgggaag gggttgaggc gcgcggacct cgcgcggcgg acgggctgca atctggagac
     3061 catccgctat tacgagaaga tcggcatgat gccggagccg ccgcgcacgg cgtccggcta
     3121 ccgcgtctat gacgagcggc acgtcgcccg cctgcgcttc gtcctgcgtg gccgggaact
     3181 gggtttctcc atcgaggaac tgcgcgggct cttgcgtcta gtcgaccggg ggacccagac
     3241 ctgcgcggaa gtcaaggcca tgacggagca gcatcttgcg gacgtgcggg ccaagatcgc
     3301 ggacctccgg cgcattgaga aggtgctcgc cgccacggcg gcgcagtgct ctggcgaaga
     3361 ggtgccggag tgccccgtgc tggaggcgct ggcatcgtga tcgcgacggt cgctgtctat
     3421 atcggcgccg ccgtcgccga gatcgccggg tgcttcgcct tctgggcgca tttccggctt
     3481 ggccgaagca tcttctggct tgcgcccggc ctcgtcagcc tcgtgctgtt cgccttcctg
     3541 ctgacccgcg tggacagcga cttcgccgga cgcgcctatg cggcctatgg cggcatctac
     3601 atcgccgcat ccatcctgtg gctctggctg gtcgaaagcc ggacgcccga tcggtgggac
     3661 ttggttggcg cggcaatctg cctcctgggg gccgccgtca tcctgtgggg gccacgcggg
     3721 acttgagcgg tcccgggaaa gcatcgactt ttccgtctgc ggggaaatcc taccctggtc
     3781 tcggcttcgc gtgctatatt gagaacaaat aaagaacgga tgtgacgtgc attgaaatca
     3841 ggacaattcg ctcccctgcc ccttgaagag attgaaccga tctcctgctc cgccgtttct
     3901 ggaggcgcgg cggcctcgtc cggcgccgtc tttcccgcaa acgacaacca tccagtccct
     3961 cggcagctaa gcaagctgtt cacctcggcc ttggccatag agctggagga ggcgcgggag
     4021 gccggcgctc tcggcttcat ggcgcgcgcc atggtccagg cgaccatgcc tcaccgcagg
     4081 atgccgggga acgagttcac gcggaccaac ggcgccttca ccatgaccat gcttgcgccc
     4141 tccaagatcg gcttgcccta cggcagcatc ccgaggctcg tcacgggctt cctgaccacg
     4201 gaggccgtcc gcaccaagag ccgcgagatc gttctcgggc ggacgctcag caagttcatg
     4261 gccgagctcg acctcgcgcc gaccggcggc cgctggggca ccatacctcg gcttcgcgac
     4321 cagatgacgc gcctgttcgc ctgctcgatc tcgtgcacct atacgagcgg caactccttc
     4381 ggcttggaaa acgtcaatat cgccgataag gccacgctct ggtggacgcc gcaccaaccg
     4441 gatcaggcgg cgttgtggga gagtaccgtc gtcctgtccg ataggttctt cacggagatc
     4501 accgagaacc cggtcccgat cgaccttcgc gccctcaagg cgctgaagcg gtccccgatg
     4561 gcgctcgatg tctatctctg gctcacctat cgcctgtcct acgtgaaggg ccggaccgct
     4621 atcccctggg ctgccttgca gctccagttc ggtgcggatt acccgaccac cgaccagggg
     4681 cgccgcgact tcaagaaggc gttcctgcgg gcactgggca aggtcgcggt cgtctatccg
     4741 gcggcgcgcc tcgatacggc acccgacagc ttgattctgc tgcccagtcg gccgcatatc
     4801 ggtcgctcgt gagacctgac cacacgcctg cgctgtcttc gcccattcta cgcataaacg
     4861 ctcccgccag ccgaccgccc tgaggtcgag gatgcacttg ccggtgtccc tgagcgccgc
     4921 cgcgctgtgc aaaaccctgt gaactagcag acttatccac gcataaacgc tcccgcgcgg
     4981 aatgcggcag gaatccttca gttctggttt tggctacgca taaacgctcc cgcggttacg
     5041 cataaacgct cccgctatat acctatatgt acctgtataa ttaaatcctg tagttggccg
     5101 cccacttctg tggatagttc gccgggtttc aggacagacg accacttccc atcatttctt
     5161 ctgccgcctg tgctcttcgg cctgttcgcg ctgtgcctgt cgctcggcga tcttctgctc
     5221 cttggcgtcg ttgaacgcct tcttgttctt gttccagaaa accaggttga ggacgacgat
     5281 gccgacgacg accacgacgg cgattgtgat gaggttctcc atcatttctg aaactcccga
     5341 tgtgctgatc cttcggcttt tggttttgag aggggtgtgt ggcgcgctga aagccctctc
     5401 agttcattct tcgaacgacg gaggcatcgg agggcgcttc gcttcctcgg atggtcctac
     5461 aggccgctca ggcggtctag ggccgttctc ggtagacggc ggattggcct caggcttttg
     5521 cgcacgcatc tccgcctgga gggcggcgag gcggcgctgg tattccggat tgcctgccag
     5581 attggcgtct tccttttgag cgccttcact cttggtgggt gctgctgatt ccgcgctgcc
     5641 ggatggcggt gcatccggtt cgtcgtcggg agcaggtatc ggtgcttccg cccgcggcgc
     5701 acgcaagacc tcctccccga aatcccgctt ctgttcggca atccatgcct taagctccgg
     5761 attaccgtgg gcaggcttca ccatccgcga gcggatcatc aggccgtcga ggaagatgac
     5821 cgcgtcttcg cggaactccc gtcccatgag cttctgcggc gaggccaccc agtcctcgcc
     5881 gccccagatg tccttccgca tgccgagccg gttggcgatc tcggtcgccg tgttcatgtc
     5941 gttcggccgg aagaagatct ggcagcggca gttggccagc aggctctccc acttgggata
     6001 gacctccttc aactgcgcca tgtcctgggt gaagagccat agccgcaccc ggtagctccg
     6061 cgtgatggcg acggcttctt cgatgatccg catgtagcca agctgcggca gctcatcgag
     6121 gtagaacatc atcggccagt cgggaaagcg ctccggcaga tcttcgtcca acctgttgtt
     6181 acgctcgata tcggcccgcc acttgatggc ctcgcccagg tgctggccca gcatcacccg
     6241 cagcaccgaa cggtaggccg caatcttctc cggcttcacg atcagataca cggacgcagc
     6301 catgccacgc tcgacatgcc caggagcggg tgagtgccag cccggcgctc gcccctgttg
     6361 ggccgcgaag gtgacgccca tgttgtcctg tacgaggatg ctctcgacag accagcccgg
     6421 cgttgtggtc gacgtgatgt ctgctatctc gtccgctcgc cagggggcta gctgggtgag
     6481 cagcgtcgtg gcaatcgacg aatgaaggtt ctccgaccgg ctttcgagga tattgcccag
     6541 ctcctgcaat cgttcttccg atgagccttg caggctttcg atcatcccgg tgaaatccgc
     6601 cttgctgagc gtcaggcagc ggcacacctc gcgcatgttc ctgcgctcag gcgggcgtgt
     6661 gcggagcaca tagaagatga gccccgtcaa taggtccttg gcggcctcgt cccagaaggg
     6721 ctcccggctt tgctggacga tgagcagttc ggcaagtagc cgcgcgtcgt cccagccttt
     6781 gactgcgtcg agtgggttgt agcaatcgga ctccggatcg atagtcgcgt cctccgactt
     6841 cggctcccat ttgtagacct ggccataaag attccggcgc cacgcggtca gttcgtagtt
     6901 ctcacccttg gggtcgaaga tcaccgccgg cccttcgtag tccagcaacg tcgggacgac
     6961 gaaacgttgc cccttgcccg cacccgtggg ggccacggtc agcaggtgtc cttcgccgtg
     7021 gaagagcagg ttcgcgggcc gtaagtcatc cccgagcggc aactcgccga tgataatcga
     7081 gtccaccggg tcgctttcgc tcgtcggcgt gatgaggccg gcctcgcgta gggcgtcgct
     7141 atcggcccac gcctcgtgct ggccggcgtg cttgacccgt tcccgcgtct ccgggctcat
     7201 gaactggaag tatttgcgta cagcctcgcg ggcgtcgtcg atccgacctt cctgaaggcg
     7261 ccggtagatg tcctgcttga agacttcgtc cacgctctta tcggccgcga tcgctgtcac
     7321 agcggcgcgc cacatcgggt cgagcgattc cagccagcgc ctgacctgag gttttccttc
     7381 cggcggcttc ttcccgcccc cgaacagact gccgaacacc atccgctacc ccgctatcgt
     7441 gcctgtctcc cttgttcgcc tgcccaaatc ttttaccccg aattggccgg ttaacccact
     7501 ctgttcagcg agttgcggct gatggcttgt gctgcctggc gcatgtcagg cgactgtctc
     7561 ggggttgagc ccataagcct gcaacgaagg taccgacagt ctccgccttg cccgcctcac
     7621 gaagaggcgg gcctcttttg tttggaccgg cgcgtcgtct cccgtcggcg cgctgctcct
     7681 cggatcctcg tccggaaccg ccgcccacgg gccgtcctca tggggtggct gacctggcag
     7741 ggacgccgtg cggcgcaacg gcggtccgtc ggtttcttcc ccggctgaca ccttcctcgc
     7801 ggacgcttcg ctccaagaaa cagcctgccc gccgtcctcc gattctctgc ggtccttcgg
     7861 atgcgccgcc cgtcttgccc ctgcctttcc ggtcgccatc ggacggccgc gatgggtgcg
     7921 gccccgcaag acaaggagag gaacaatgac cgcgcaacgc atcgacgttt acacccaggt
     7981 caccaacaac atcatcgccg ccatcgaggc gggagcgggc gactggcaga tgccctggca
     8041 ccgcagcggc gatggcctca accgccccgt caacatcgac accaacaacg cctatcgcgg
     8101 catcaacgtc gtcagcctct gggccgcagg ccaggcccgc ggcttcagca ccggcacgtg
     8161 gggcacctat cgccagtggc agaacaacgg ggcgcaagtc cgcaagggcg agaaggcgag
     8221 cctggtggtc ttctacaagg agttcgacgt ggaagaggcc acggacgacg gcgaaaccga
     8281 gcagggcaag cgcctcatgg cccgtgcgag ctgggtcttc aacgccgacc aggtggacgg
     8341 ctacgaggcg cccgcgctgc ccgagcccaa ggacccagtg caggtcctcg acaaggccga
     8401 gcgcttcatc gccgataccg gcgccatcgt ccaccacgga ggcagccgcg ccttctaccg
     8461 cccgtcggac gacatcatcc agatgcccga gcgggagcgc tttctcggca ccgagacaag
     8521 cacggcgacc gagagctact acgccacgct cctgcacgaa ctgacccact ggacgggcca
     8581 cgaccgccgc tgcgaccgcc gattcggcaa gcgcttcggc gacgatgcct acgccatgga
     8641 agagctcgtg gccgaactgg gcgcggcctt cctctgcgcc gacttgggcg tgacgctcac
     8701 accgcgtccc gaccacgccg cctacatcga caactggctg aaggtcctca aggctgacaa
     8761 gaaggccatc ttcaccgccg cctcacaagc ggctaaggcc accgactacc tcggtggcct
     8821 gcaatccgcc gacatcaccg aggccgccgc ttaggcggcc tcttctgtca cgcgcccagt
     8881 tgtctgagtt cggactcaag cagttgcctt tctgaccgga ggtccgcttt tggccgatcg
     8941 gcgcgcgttt ccagttcctt ctcaatctcg acgaggcggg tgcggatggt ggccacgcgg
     9001 tcgagcacaa tgcgtccaaa gttgcgtgcc gttacgaacc agaacccacc cgccacgagc
     9061 gtcagaagcc aaagcagacc aacgcctatg aaccaccaac ctaattgctc ccagacgtag
     9121 ccgtcgtatc ggagcgccca gttcgacgtg ttgtttatct tgatcaggag cacgacggtg
     9181 acgagaacag ccggtactgc gagcacgatc gcgatggcgc gagcgctttt catttgagca
     9241 cgcagcttct gcatcccctc cggcgacgcc cttatttcgg caaagaaagg gccatgtcgc
     9301 tgagcccgat tgcgtagctt cttacgaagg aagagcgctg cgaccacgac cagcagaacg
     9361 accagcgcgt caagaagtcc gtcaagaatg ccgccggaat tggaagacgc cgcttgagca
     9421 aaggccctgt gaattggcgt gaccgacagc gccaccaaga gcgacagcgt tgcgggtcgc
     9481 aggaccattg ctctcatggc ttccacacct gctggtcgtt ctcgaagaag atgaagcagt
     9541 cgtgggccgg attgtaggca ggcttcttcc ggcagctcgc gagcgcgtct tcacgcatct
     9601 ttgccatcga cgcaccgacc ttgtaccgcg cgccataggc gccgtccggc gagacggcga
     9661 acgccttggg gaaggggctc ggcccatagt tggtgaggaa gcaatcgacg cacgccttgc
     9721 cgccgcccgc ctgttcgagc gcgggcacgg cggccttgta ggccgccagc ggatcggcat
     9781 ctgcgctttt tgcactggtg gcgccggtcg tctggcaggc ggtgacggtg aggagcgcaa
     9841 tggcgatcag ccaacccttg gacaaatacg tgttccgcat ctggagcgcc tcagccaagg
     9901 gcttcccagc gttcccaaac cggggcgccc tggtccggtc gggtctgttt ctggtaggcc
     9961 gggctcttgg ggttgttgca ggtgcccttg ccggaagcga taacggtctt gcggtcttcg
    10021 gacaccgaac gggtaccgcc ccagaaacgg caagtcccgc atttctgcgt cgccgtggcc
    10081 ggtaagttga cctccgccat tgcaggctga ctggcgatgg tgccgccgac cgtggccagc
    10141 cccgccacgc cgagcgcccg gagaacgtcg cgacggcttg ccgaccgcgc aacaactttg
    10201 tctttggtgt tcatggaaac tcctaacctt gaacggatga tccaaggcgc gtgcggtgct
    10261 gacacgcgcg cgtgaaaagc ggcccgtttg gacaagcccc cgatttcctt cctagaaggg
    10321 ccgcttgtgc ggcgtcacgt ccaaagttgg aagggttgga aaatccggaa aacaggcgga
    10381 atccatgatc gttatcaaca ggttgacgga ttccggcagg gtgggtagaa gttgaagaac
    10441 gaggtgattg gccgggggcg gcatgtcagg cgcgttcgac agtttcgggg aatggcacag
    10501 gctattcgat gccctttgca tcgagcgcgg ccatctcgat aacgcgacgc ttgccagcga
    10561 atactgcgcg ctcaccaaga acaaggcatc taccgcttac gagagttccc tgaagagcct
    10621 gaacaactgg cggcagggac tacatacgcc cagccgccgc aacttcagaa tactgacatt
    10681 aatccttcgc gttgaagacg cgccggaggt gttgcctcac tggagccggc tctatgaaga
    10741 ggctcagagg cgcaagcccg ccgatggcaa tggcgatgcg agccagaccg cgacaatacc
    10801 cgagatcgac ccagccccta ccgccgcatc cggcggacgc gccagccgac gcttagccgc
    10861 cgctgccgtt ttgctgttct tgggggcggg cggggcggtt cttgcgttgg gcaccttcga
    10921 cggcccccag acgcatgctg cggcattcac gcagccaggt gcgcaggtcc ttgacatgac
    10981 cggccagcgt atcgaggcgc atgagatggt caccgccaaa gtcggccagt ctgtggtcgt
    11041 acacggcagc cgcgggcggt gtggtgaaca gccgccagat tgggaggagg tcttccaagg
    11101 actgcctccg ctatctaacg gtgtttggtc ggacgggggc gtcggcatcc gcgtcagccg
    11161 cgcttgcagc ggcggcacgc ctgcgcgagc cgtggtgttc acggcaacgc atccgggcga
    11221 ggaacgattc accctctacg acgatccgat tacgatccgg gtgaccgagt agagcattcc
    11281 gtcgcttcgc gttatcggag ggctggagca atccggcctg ttttgttctt gctacgcttg
    11341 ggcggcccca gtgtgatcgg
//
LOCUS       DASVDU010000003         8381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1537081, whole genome shotgun sequence.
ACCESSION   DASVDU010000003 DASVDU010000000
VERSION     DASVDU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537081"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VF226_00090"
     CDS             complement(<1..997)
                     /locus_tag="VF226_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX7072418.1"
                     /translation="MLQKTYQPADIEKRIYQEWENAGAFRAGRPERVSAKPYCIVIPP
                     PNVTGSLHMGHALNNTLQDILCRFERMRGKDVLWQPGTDHAGIATQMVVERKLAKEQR
                     DRRQMGRQAFLEEVWKWKAESGGMILNQLKRLGASCDWSRERFTMDEGLSRAVLKVFV
                     ELYKRGLIYKDKRLVNWDPRFQSAISDLEVMQVEVKGHLWHLRYPLKDDPSRFIVVAT
                     TRPETMLGDTAVAVHPEDERYKELVGKMVVLPLVGREIPIVADEYSDPEKGSGAVKIT
                     PAHDFNDFEVGRRHNLPMINIFDAEARINENAPEAYRGLDRFEARKRVIADLEAQG"
     gene            complement(1164..2384)
                     /locus_tag="VF226_00095"
     CDS             complement(1164..2384)
                     /locus_tag="VF226_00095"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX7072419.1"
                     /translation="MHRADKTPEPSMEEILASIRKIIAEEPIGSRPGPEPRETESDRQ
                     SSTGGSGRQANEPTLSIPSRNEDHPEEPPYTVEDALADLMDDPSQRRPGRPAGKDDLP
                     KPQARPAGEEESRPSWLFGRQTSPPAGASQSPGAIPAGSDRRQGGGLLGHLGQLRETP
                     ERAAGEGEVSSPKPSTGSPRPLEAGSLFERQREGGRPGPADFPSLPQVQSQDVPRPSA
                     PRVPTREATIDRRADEGHGLMAPGAGSAGAKHGSPAPMARSPEPAPDRPIHPQTPARA
                     PIAEAERKPEERQRPAPTAADGLVRQEKAVPAPSADHPGGEGPQREKLAEGAKTVAQN
                     ASPASGNAQLAPSQAPQAVQPAAARTLEDTVADLLRPLLREWLDANMPRIVEKALRVE
                     LAASAKRPEIDSNR"
     gene            complement(2690..4156)
                     /locus_tag="VF226_00100"
     CDS             complement(2690..4156)
                     /locus_tag="VF226_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX7072420.1"
                     /translation="MAAKTEGEMGVSRKRIARAAIAAVCAAMLIVPAGVADAETLREA
                     LTAAYKFNPRLDAERANLRATDEEVPRALSGYRPQIFGSADVGYQRSKTRGGGQTNVQ
                     ETNPRGYGVDISQPIFRGFRTLNTVREAEAVVRAERANLLIVEQAVLLEAVIAYADVI
                     RDQAVVRLRENNVNVLTRELRATQDRFAVGEVTRTDVAQAEARRSGAVSALDLARANL
                     RTSRAAFERVIGHPPSNLADPQVPEKLLPRSQEEALAIASRENPNLVAALYREQAARH
                     VVDRIWGELLPEVSVDASYDRRFGQGNFDESEVTAVTGRVTVPIYQGGEVSARVRQAK
                     HTHVARLQEVEQFRTETVADVVASWARMEAARAQIASDQAQVEANRVALAGVREEERV
                     GQRTLLDVLNAEQELLNAEVNLVTTRRDLVVSAYTLLASIGRLDAEYLGLGAAIYDPE
                     AHYQEVRRKWWGISITHADGRRENLDLWDTYGKNVPMK"
     gene            complement(4368..5027)
                     /locus_tag="VF226_00105"
     CDS             complement(4368..5027)
                     /locus_tag="VF226_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX7072421.1"
                     /translation="MVSFAVQRENMVESQVRTADVVDRRIIRAMLEIPREAFVPEDVR
                     ALAYMDGDVPITQSQSRFMMAPGVHAKLIQFLEIEDNAVVLDVGCGTGYGSAIMARLA
                     QTVVGLESDPAIAESATKILSELGVDNVVIVIGELTAGWPSEGPYDAILLNGSVPEVP
                     SVLLEQLKDGGRLAAIEADEGVSRARIWQRSGKTVSARTVFDAAAKPLPGFERKPEFV
                     F"
     gene            5269..5342
                     /locus_tag="VF226_00110"
     tRNA            5269..5342
                     /locus_tag="VF226_00110"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5301..5303,aa:Cys,seq:gca)
     gene            complement(5775..6575)
                     /locus_tag="VF226_00115"
     CDS             complement(5775..6575)
                     /locus_tag="VF226_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX7072422.1"
                     /translation="MSLEPYEVYAVRYAHNPAARSSHNFVLGDPHDVPMPLDYFVWVI
                     KGNGHTFIVDTGFDAAMAKKRKRDFLRSPGEGLKAIGVDPDAIKDVILTHMHYDHCGN
                     YDLFPNAKYHLQDIEMAYCTGRCMCHDALRAPFEEEDVVAMVRKVFAGRVRFHDGDSE
                     IADGITVHHVGGHSKGLQVVRVWTRRGWLVVASDASHFYANMEQGRAFPIMHSLADTL
                     EGYDRLWKLASDRTNVIPGHDPIILDRYPAPSSGLEGIVVRLDADPRS"
     gene            complement(6647..6853)
                     /locus_tag="VF226_00120"
     CDS             complement(6647..6853)
                     /locus_tag="VF226_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072423.1"
                     /translation="MAEFPDEGWKVTGSYDYSCTPAMTEAIAKEIPGCRYRQMKNIGH
                     FQMIENYELFRTYLLPEIEIMAAE"
     gene            complement(6957..7355)
                     /locus_tag="VF226_00125"
     CDS             complement(6957..7355)
                     /locus_tag="VF226_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7072424.1"
                     /translation="MEIKRCGSRPTRRAPASAFTGTVWQDPVIEAPEPALLKSNVVTF
                     EPGARTAWHTHPLGQTLYVVSGTGRIQVWGGPIEEIKAGDVVWIPPGEKHWHGAGPTT
                     MMTHISMQEARDGVAVEWLEHVTDEQYAGA"
     gene            complement(7443..8333)
                     /locus_tag="VF226_00130"
     CDS             complement(7443..8333)
                     /locus_tag="VF226_00130"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7072425.1"
                     /translation="MSETFGFIGLGNMGRPMAGRLIDAGHSLVVHDVRESAVVDFVGR
                     GAKKANSPAELASMVETIFLSLPTPPVVEAVAVGENGLMSGEKVRRVVDLSTTGPQTA
                     ESISEKFQSRNIRWLDCPVSGGVGGAKAGTVAVMFSGPSADYEDLLPVLKVIGKPFYI
                     GDKPGLAQMMKLVNNCLSAAAMALSSEAVVMGAKAGIPAKVMIDVINAGSGRNTATLQ
                     KFPQSILPGTFDYGFATGLMHKDVSLFMQEARRMGLSLEGCQVVFDIWQKALDKLGPD
                     SDFTRIVTLMEEQAGVEVRG"
BASE COUNT         1448 a         2814 c         2525 g         1594 t
ORIGIN      
        1 gcccctgcgc ttcgaggtcg gcgatcaccc gctttctcgc ctcgaagcgg tcgaggccgc
       61 gataggcctc cggcgcgttc tcgttgatcc gcgcctcggc gtcgaagatg ttgatcatcg
      121 gcaggttgtg ccggcggccg acctcgaagt cgttgaagtc gtgggccggc gtgatcttga
      181 cggcacctga gcccttctcg gggtcagagt actcgtcggc cacgatcggg atctcgcgcc
      241 cgaccagggg cagcacaacc atcttgccga ccaactcctt gtagcgctcg tcctccgggt
      301 gaacggcaac cgccgtatcg ccgagcatcg tctcgggccg cgtggtggcg acgacgatga
      361 agcgcgaggg atcgtctttc agcggatagc gcaggtgcca gaggtggccc ttcacctcga
      421 cctgcatgac ctcgagatcg gaaatggccg actggaagcg cgggtcccag ttcacaagcc
      481 gcttgtcctt gtagatgagg ccgcgcttgt agagctcgac gaagaccttg agcaccgcac
      541 ggctcagccc ctcgtccatg gtgaagcgct cgcgcgacca gtcgcaggag gcccccagcc
      601 gcttcagctg gttgaggatc atgccgccgc tttccgcctt ccacttccag acctcctcga
      661 ggaaagcctg gcggcccatc tggcggcggt cgcgctgctc tttcgcaagc ttccgctcca
      721 ccaccatctg ggttgcaatg ccggcgtggt ccgtgccggg ctgccagagc acatccttgc
      781 cgcgcatgcg ctcgaaccgg cacaggatgt cctgcagcgt gttgttgagt gcatgcccca
      841 tgtgaagcga gccggtgacg tttggtggtg gaatgacgat gcaataaggc ttggcgctca
      901 cccgctccgg ccggccggcg cggaacgcgc cagcgttctc ccattcctgg tagatccgct
      961 tctcgatgtc ggcaggttgg tacgttttct ggagcatggg tcggacggcc cggtttcaaa
     1021 tcaaggaaag cgaagggcgt cctacgcccc gccttccttc cctgtcaact cgagtggtcg
     1081 cctcagggga cctggataca aacgcgcagc gcgcacagaa cccgcgccgt ctcggcaacg
     1141 acgagccagc tgtcagccga agctcagcga tttgagtcga tttccggccg cttggcgctg
     1201 gccgccaact cgacccgtag cgccttctcg acgatgcggg gcatgttggc gtcgagccat
     1261 tcacgcagca agggccggag caggtcggca accgtgtcct ccagggtgcg agccgctgcc
     1321 ggctggacag cctgcggcgc ttgtgacggt gccagttgcg cgttacccga ggcagggctg
     1381 gcgttctgcg caaccgtctt ggccccctcc gccagctttt cccgctgggg tccttcgccg
     1441 ccgggatggt cagcggaggg ggccggaacg gccttctcct gccgcaccaa accgtcggct
     1501 gcagtcgggg ccggccgctg gcgctcttcg ggcttgcgct ccgcttcggc tatgggcgcg
     1561 cgagccgggg tctgcgggtg gattgggcga tcaggcgccg gttccggtga acgcgccatg
     1621 ggcgcaggcg aaccgtgctt ggcgcccgca gagcctgcgc caggcgccat gagaccgtgc
     1681 ccctcgtcgg ctcggcgatc gattgtggct tcccgcgtcg ggacacgtgg cgcagaaggc
     1741 cgtggcacgt cctgggactg gacctgcggc aaagacggaa aatctgctgg accgggacgc
     1801 ccgccctccc gctggcgctc gaagagcgag ccggcttcca gcggtcttgg gctgcccgtg
     1861 ctcggcttcg ggctggaaac ctcgccctcg cccgccgccc gctcgggcgt ttcgcgcagc
     1921 tgaccaagat gccccagaag gcctcctcct tgcctgcggt cagacccggc cgggatcgcg
     1981 ccgggagact gggacgcacc agcaggcgga ctggtctggc ggccgaacag ccaactggga
     2041 cggctctcct cctcgcctgc tggccgggcc tgcggcttgg gaaggtcatc cttacccgca
     2101 ggacggccgg gtcgccgctg cgacgggtcg tccatcagat cggccagcgc atcctcgaca
     2161 gtgtaaggcg gctcctcggg gtggtcctca ttccgtgagg ggatggaaag agtgggttca
     2221 ttcgcttggc gcccagaacc accggtcgac gattgacgat cgctttcggt ctcccgcggc
     2281 tccggtcctg gccgcgagcc gatcggctcc tccgcgatga tcttgcggat cgacgctagg
     2341 atttcttcca tgctcggctc gggcgtctta tccgctctgt gcatccccca ccctcactgc
     2401 tctacggttc tcgcccggcc aaagccgcgt gcccaacctg caacgcacga caaagatttt
     2461 ggtacaccga tcgctcggtt gcatcaatgt attggcttca tagaaattgt gcgttttgtg
     2521 aggctttccg atgcatctaa tgccacaccc caatggcacg aaccagcagc cgtccgcgtc
     2581 gctggacttg gctccatgcg actatcggtt agctagctac taggagcatg caacagggag
     2641 ggcctcgcgg gctaccggcg cgcggtcagg cggaccagcg cactcgggct cacttcatcg
     2701 gcacgttctt tccgtaggtg tcccacagat cgaggttctc acggcgtcca tccgcatggg
     2761 ttatgctgat gccccaccac ttgcggcgga cctcctggta gtgggcctcc ggatcgtaga
     2821 tcgccgcgcc gagccccaga tactccgcat caagccggcc gatggacgcc agcagcgtgt
     2881 aggcgctgac caccagatcg cgccgtgtgg tcacgaggtt gacctcggcg ttgagcaatt
     2941 cctgctcggc attgagcaca tcgagcagcg tccgctgacc aacgcgctcc tcctcacgca
     3001 cacctgccag ggccacccgg ttcgcttcga cctgcgcctg gtcggatgca atctgcgcac
     3061 gtgccgcctc catccgcgcc caggacgcga ccacgtcggc caccgtctcg gtccggaact
     3121 gctcgacctc ctgcaggcgc gcgacgtggg tgtgcttggc ctgccggaca cgagccgaga
     3181 cctctccccc ctgatagatg ggcacggtca cgcggcccgt gaccgccgta acctcgctct
     3241 cgtcgaagtt gccctggccg aagcggcgat catagctcgc atcgacactc acttccggca
     3301 gcagctcgcc ccagatccga tcgaccacat ggcgcgccgc ctgctcgcgg tagagtgcgg
     3361 caacgaggtt cgggttctcc cggcttgcaa tcgccagcgc ctcctcctgc gagcggggca
     3421 gcagtttttc cggaacctgg ggatccgcca ggttgcttgg cggatgtcca atcacgcgct
     3481 cgaacgccgc acggctggtg cgcaggttgg cgcgcgccag atcgagcgcg gacacggctc
     3541 ccgaccgacg cgcctcggcc tgcgcaacgt ccgtgcgcgt cacctcgccc accgcgaacc
     3601 ggtcttgggt agcgcgcaac tcgcgggtca gcacattgac gttgttttca cggaggcgga
     3661 cgacggcctg atcccgtatg acatctgcgt aagcgatcac ggcctcgagc agcaccgcct
     3721 gctcgacgat cagcagattg gcgcgctctg cccgcacgac agcctcggcc tctctcacgg
     3781 tgttcagcgt ccgaaacccg cgaaatatcg gctgagagat gtcgactccg tatccgcgcg
     3841 ggttcgtctc ctggacgttc gtctgtccgc ccccgcgggt tttcgaccgc tgatagccga
     3901 catctgccga gccgaagatc tgcgggcgat agccggacag cgcccgcggc acctcttcgt
     3961 cggtcgcgcg cagattggcc cgttcggcat cgaggcgagg attgaacttg taggccgcag
     4021 tcaaggcctc acgcagcgtt tccgcgtctg cgacaccagc aggcacgatg agcatcgccg
     4081 cacatacggc agcgatcgct gcccgtgcga ttctctttcg agacaccccc atttcgccct
     4141 cggttttcgc tgccattcag ccccccactg tccaggacgc aatcaccggg caaggccgat
     4201 actcctggcc tgctccaggg gcttacactg attccatcca gacgcgcacc ggatcgaatc
     4261 tctgagtacc ccgacaggac aacaacttag aatagcgaat ggcgccgaaa gcacagatac
     4321 ttggccggcg tggccgtaac cccacacttc ggccacgata cggatgatca gaatacgaac
     4381 tctggcttcc gctcgaaccc tggaagcggc ttggcggctg catcgaacac ggtccgcgcc
     4441 gaaacggtct tgcccgagcg ctgccagatc cgggccctgc tcacaccctc atcagcttcg
     4501 attgctgcaa ggcggccacc atccttcaac tgctccagca gtacggaagg cacctcaggc
     4561 accgacccgt tgagcaggat ggcatcgtag ggcccttcgc tcggccagcc ggcagtcagc
     4621 tcgccgatca cgatcaccac gttgtccacc cccagctcag agaggatttt cgtcgcgctc
     4681 tccgcgatcg ccgggtcgct ttcgaggccg acgacggtct gcgccagccg tgccatgatg
     4741 gcactgccgt aaccggttcc gcagccgacg tcgagcacga ccgcattgtc ctcgatctcc
     4801 aggaactgga tcagcttggc gtgcacgccc ggtgccatca tgaaccgcga ttgcgattgg
     4861 gtgatgggga catcgccatc catataggcg agtgcccgca cgtcctcggg aacgaacgct
     4921 tccctgggga tttcgagcat ggcgcgaatg atgcgccggt ccacgacatc ggcggtccga
     4981 acctggcttt cgaccatgtt ctcgcgctgg acggcgaaac tgaccatctc ccctcctcga
     5041 cactcccgtg caaagcatac ggctggcata gccaaagcgg cgggaactta accaagcaat
     5101 gcccgctccg ccagagccct tgttcaggta gaaggccgac tcggacgcaa agccgcacgg
     5161 gagcgacctc attgcaggac cgtcccgggc atgctacagg acgccttcgc ccgagaggct
     5221 cctcgcggcg gcggctgaag tgcgcactgc acctcgccca ttcttggcgg ccacgtggcg
     5281 gagtggttac gcagcggact gcaaatccgc gcacgccggt tcgattccgg ccgtggcctc
     5341 cagtaaattc caagagaagc agtgcagagt gtgaccgctg tcgcggccgt cgcgccactc
     5401 gcgccctacg tcttggagca aattcagggg cagcgtgcgt ggcatgtgtc gcggcagttc
     5461 ggccgctcgc gccaccatgg ggcggtgatc gcggaggatc gccctcatct ggtcccttgg
     5521 atcaaatttc ggccttgcac ccatcgtcgt cgaccaggtc cacgatgcgc cgatcaagag
     5581 tcatcaaagc ggcacctcga tcctgctggt cgagcaaaac accgcccttg cgctctccgt
     5641 ggcgtcgcgg ggctatgtgc tggaatcggg ccggatcgtg ctaagcggtg cggccgagtc
     5701 gctgcacaag aatccaaggg tccgtgaagc gtatctcggc ctctgagcgc gacatggcat
     5761 tgtctcaatg ccgcctacga cctgggatcg gcgtcgagcc gcacgacaat gccctccaat
     5821 ccggaagagg gtgccggata gcggtctagg ataatcgggt cgtgacccgg aatcacattc
     5881 gtccggtcgc tcgccaattt ccatagccga tcataccctt ccagcgtatc ggccaggctg
     5941 tgcatgatcg gaaaggcgcg cccctgctcc atattggcgt agaaatggct ggcgtcggaa
     6001 gccacaacca gccagccgcg ccgcgtccag acgcgtacca cctgcaggcc cttgctgtgt
     6061 ccaccgacgt gatggacggt tatcccatct gcaatttcgg aatcaccgtc atggaatcgc
     6121 acccgaccgg cgaaaacctt gcgcaccatc gccacgacgt cctcctcttc gaagggcgcc
     6181 cttaacgcgt cgtggcacat gcatcggccg gtgcaataag ccatttcgat gtcttgcagg
     6241 tgatatttcg cgttcgggaa gagatcgtaa ttcccacagt ggtcgtaatg catatgggtg
     6301 aggatcacgt ccttgatcgc atccgggtcg acaccgatcg ccttcaatcc ctctcccggc
     6361 gagcgcagga agtcgcgctt tctcttcttc gccatggccg cgtcgaaacc ggtatcgaca
     6421 atgaaagtgt gcccattgcc ctttatcacc cagacgaaat agtcaagcgg catcggcacg
     6481 tcatgcggat cgcccagaac gaagttatga gatgaccgcg cggcagggtt gtgggcgtag
     6541 cgaacagcgt agacttcata aggttcaagc gacatgtgca ttcctccaag gacaaccgaa
     6601 ccgcgtcggc gctcgggcca attatcgatt cagccgtaat cgattgctac tcggcggcca
     6661 tgatctctat ttccgggagc agatatgtcc ggaacagctc gtaattctcg atcatttgga
     6721 aatgcccgat gttcttcatt tgcctatagc ggcaacctgg gatctctttc gctatggctt
     6781 cggtcattgc cggtgtacag gagtaatcgt agctgccggt gaccttccag ccctcatccg
     6841 gaaactcagc cattctcatt ctcctcccgc cgctccggtg cagtgatggc ggcgccggtc
     6901 agcctcacgt catgccgtta aatgaagtga ggcggtgtcc gtccgctcag tcgatgtcag
     6961 gcgcccgcat actgctcgtc ggtgacgtgc tccagccact caacggcgac gccgtcacga
     7021 gcctcctgca tcgagatgtg cgtcatcatg gtcgtcggtc cggcaccatg ccaatgtttc
     7081 tcgcccggcg gaatccagac gacgtctcct gccttgattt cctcgatagg accgccccac
     7141 acctgaatgc ggccggtgcc cgagacgaca tagagcgtct ggcccaatgg gtgcgtgtgc
     7201 caggcggtgc gcgctccggg ctcgaaggtg acgacatttg atttcaacag tgcgggctcg
     7261 ggtgcctcga tgaccggatc ctgccaaacc gtgccggtga acgcgctcgc cggggcacgc
     7321 cgtgtcggcc tcgagccgca acgcttgatc tccatcctgt gctctcctct cctctgaccg
     7381 gactggaccc cgttgtctcg ctgagataag cgcatacacc ggcgcagctc ccagctcacc
     7441 tatcagccac ggacttccac acccgcctgc tcttccatga gggtgacgat tcttgtgaaa
     7501 tcggaatccg ggccaagctt gtcgagcgcc ttttgccaaa tgtcgaaaac aacctggcag
     7561 ccctcgagag aaagccccat ccgcctggct tcctgcatga acagcgacac gtccttgtgc
     7621 atgaggcctg tcgcgaagcc gtaatcgaac gtgcccggca gaatggattg gggaaacttc
     7681 tgcagcgttg ccgtattgcg gccgctgccg gcgttaatca cgtcgatcat cacttttgcc
     7741 gggatgccgg ccttcgcgcc catgacgaca gcttctgagg acagtgccat cgctgccgcg
     7801 gagaggcagt tgttgacgag cttcatcatc tgcgcgaggc ccggcttgtc gccgatgtag
     7861 aagggcttgc cgatcacctt gagcaccggc aagaggtctt cataatccgc acttggcccc
     7921 gagaacatca cggccacggt gccagccttg gctccgccga caccaccgct gaccgggcaa
     7981 tcgagccagc gaatgttgcg cgactggaat ttctcgctga tgctctccgc cgtttgcgga
     8041 ccggtcgtgg acaggtcgac gacccgcctc accttttctc ccgacatgag gccgttctcg
     8101 ccaaccgcca ctgcctcgac gaccggcggg gtcggcaggg agaggaagat cgtctccacc
     8161 atcgacgcga gctcggccgg agaattggcc ttttttgcgc cccgtccgac gaagtccacg
     8221 acggcgctct cgcgcacgtc gtggacaacg agagaatggc cggcgtcgat caggcgcccg
     8281 gccatcggcc gacccatgtt accgaggccg ataaatccga atgtctcgct caatgcttac
     8341 ctccagatcg cccgaatgca ccgtgcattg cgacagtgcc g
//
LOCUS       DASVDU010000004         7175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_180347, whole genome shotgun sequence.
ACCESSION   DASVDU010000004 DASVDU010000000
VERSION     DASVDU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7175
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180347"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VF226_00135"
     CDS             <1..558
                     /locus_tag="VF226_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7072426.1"
                     /translation="GCAGALLLAALYRALTFERLKEATYLTARTSAMVCYLFIGSWTF
                     SSVFSYLGGHEVIEAWVKSLELSPTMFLILAQIIIFLLGWPLEWTEIIIIFVPIFLPL
                     LKAFNIDPIIFGILVALNIQTSFNTPPMAMAAYYLKGIAPPHVKLTEIFAGALPFVFL
                     VFITMAVVYIYPGIALWLPDQLYGR"
     gene            562..1905
                     /locus_tag="VF226_00140"
     CDS             562..1905
                     /locus_tag="VF226_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7072427.1"
                     /translation="MADSLTALTATEAHREITRGALSAEEYVTACLDRIEALDGDVRA
                     FVHLDREYALQQARDRDEWRRNGRPTGLLHGIPVAIKDIIDTADYPTECGSPLFKGRR
                     PRQDATVVAKLRAAGAVIIGKTVTTEFAFYHPGPTRNPHDLERTPGGSSSGSAAAVAA
                     GMVPLALGSQTNGSVIRPASFCGVFAAKPTHGTISRAGMLELSRTLDHVGVFARTLED
                     VALLLDVLAGHDPRDPDTRPVATPNFRAVAAEPPPLPPKFALVKTPVWEKADASTREI
                     FEDLISSLGENGGVAELPEAFSEAWQAHQTIMSIDMAYRLGGAVDRGSKEASSQRLRD
                     IIAEGRQRLAIDYLAAHDLRPRLESGLSPLFDYYDAIITPASVGSAPKSLDSTGDPVF
                     CTLWTLLGVPALSLPVLQAPDGMPLGLQLVGPANDDARLLRSARALIEMLSKDEG"
     gene            complement(1930..2115)
                     /locus_tag="VF226_00145"
     CDS             complement(1930..2115)
                     /locus_tag="VF226_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072428.1"
                     /translation="MADAVSAIIGMVLMIGFVVAIVVTVAEIPLWIVSILAFILMAIA
                     FWQDAFAPLLRGPRNWH"
     gene            2284..2643
                     /locus_tag="VF226_00150"
     CDS             2284..2643
                     /locus_tag="VF226_00150"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7072429.1"
                     /translation="MIAVIFEVTPAEGRWQEYLDLAAELKPQLEKMDGFISVERFQSL
                     SDPKKILSLSIWRDEEAVARWRNYPAHRRTQAKGRAGVFAGYRLRVASIIRDYEMTER
                     REEAPTDSKAVFDAKGQ"
     gene            3484..4170
                     /locus_tag="VF226_00155"
     CDS             3484..4170
                     /locus_tag="VF226_00155"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7072430.1"
                     /translation="MKAHEIMSRDVVTVKPATPVRDVAALMTEKRISGIPVVSEDGTV
                     LGIISESDLLRRAEIGTVPQRKWWLVFFSDPDALARQYTKTHGLRAENVMTRPVISVG
                     EDADLSEIARTLDKHKIKRLPVLRDGKLVGIISRADIVRALSQAEVERRPMSDAALEK
                     TLLEKMRAQDWLEDTFINLSVHDGVVELNGFVASAEQRRALHVLIEETDGVHKIDDRL
                     SIGLPALRAT"
     gene            4432..4686
                     /locus_tag="VF226_00160"
     CDS             4432..4686
                     /locus_tag="VF226_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072431.1"
                     /translation="MSKTMIIGLTAGLMALSALQAPAAEAKGKGFYHHKHGKHHGFVW
                     HKWHKPHFVHYDGCGFYFYKWKHTGKHFWKTKYFVCKGFY"
     gene            complement(4828..5157)
                     /locus_tag="VF226_00165"
     CDS             complement(4828..5157)
                     /locus_tag="VF226_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7072432.1"
                     /translation="MRNPTLIVTIVRKGWGQPVLEASIKAGATGGTVIFGRGIGVNEK
                     DTIFGIPIEPEKEIIFTLVREDEAEAILAEIIQAAELDEPGRGLAFTVPVGKVVGMPH
                     IDTQSVE"
     gene            complement(5160..5837)
                     /locus_tag="VF226_00170"
     CDS             complement(5160..5837)
                     /locus_tag="VF226_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX7072433.1"
                     /translation="MAVLPLAALFVAFQLFLLGLPRKQIRGILIGTLFATAGLFLFLL
                     GVGIGFLPFGRVIGAALGGMQEPWVLLAVGLFLGFVTTWGEPAVRILAGEIEHASNGS
                     IPQSLVVSTICIGVSVSVALGLLRIVYEISILYLLVPGYALAVILTWLSSKEFVAIAI
                     DSGGVATGPLANTFLLALALGASASNGTQDVMVQGLGLVALIALAPILSVMLLGLLIR
                     WKASAKV"
     gene            complement(5888..6583)
                     /locus_tag="VF226_00175"
     CDS             complement(5888..6583)
                     /locus_tag="VF226_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX7072434.1"
                     /translation="MWSELKRRLVDVLQTVAPLIGAVCLLQFTLVQAPAAVFVQFLAG
                     SVLIIAGMFLLFLGVDYGILPMGRFVGAELPRKGSVALIVLVGFAMGFATTVAEPDVL
                     VLAAQVDEISRGDINRLIVLAVTALGVATFVALAMARIIYDISIRNLLTASLIAVLAL
                     SYVTPEKFVPMAYDAGSVTTGVLTTPVVISIAIGLTSVLAGRSTVSDGFGLLGFASIG
                     AIIAVMIMGLAIQ"
     gene            complement(6806..>7175)
                     /locus_tag="VF226_00180"
     CDS             complement(6806..>7175)
                     /locus_tag="VF226_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7072435.1"
                     /translation="PGPGLAIRVPGEVTREKLDILRRADAIYLEEIRKAGLYDTIWQA
                     FAVLLPVRTVGVMGDARTYDHVCALRAVTSTDGMTADYYPFPHDFLGRTATRIINEVQ
                     GINRVVYDITSKPPGTIEWE"
BASE COUNT         1476 a         2240 c         2174 g         1285 t
ORIGIN      
        1 gggtgtgcag gtgcactgct gctggcggcg ctttatcgcg cgctgacgtt cgagcggctg
       61 aaggaagcga cctatcttac ggcgcggact tccgccatgg tctgctatct gttcatcgga
      121 tcgtggacct tctcttcggt cttctcctac cttggcggcc acgaggtgat cgaggcttgg
      181 gtgaagtcgc tcgagctgtc gccgacgatg ttcctcatcc tggctcagat catcatcttc
      241 ctgctgggct ggccacttga gtggacggag atcatcatca tcttcgtgcc gatcttcctg
      301 ccattgctga aggcgttcaa tatcgatccg atcatcttcg gcattctggt ggcattgaac
      361 atccagacct cgttcaatac cccgccgatg gccatggctg cgtactatct gaaggggatt
      421 gcgccgccgc atgtgaagct gaccgaaatt tttgccggcg cgcttccgtt cgtgttcctg
      481 gtcttcatta cgatggcggt ggtgtatata tacccgggga tcgcactctg gctgccggat
      541 cagctctacg ggcgctgagg catggctgac tctttgaccg ctctgaccgc aaccgaagcg
      601 caccgcgaga tcactcgcgg tgcgctttct gcagaggaat acgtcacggc atgcctcgac
      661 cggatcgagg ccttggacgg tgacgtccgc gcgttcgtcc atctcgaccg ggagtacgcc
      721 ttgcagcagg cgcgcgaccg cgacgagtgg cgccgcaacg ggcggccaac cgggctgctc
      781 cacggcattc cggtggcgat caaggacatc atcgatactg ccgattaccc gacggagtgc
      841 ggatcacccc tgttcaaagg gcgtcgtccg cgccaggacg cgacggtcgt tgcgaagctg
      901 cgcgcggccg gggccgtcat catcggcaag acggtgacga ccgagttcgc cttctatcat
      961 ccaggtccga cgcgcaaccc gcatgatctc gaacgcacgc ccggcgggtc gtcatcgggc
     1021 tctgcggctg ccgtggctgc gggcatggtg ccgttggcgc tcggctcgca gacgaacggc
     1081 tcggtcattc ggccggcgtc cttttgcggc gtcttcgccg ccaagccgac gcacggaacg
     1141 atttcgcgcg ctggcatgct ggagctgtcc cgcacgctcg atcatgtcgg tgtgttcgcg
     1201 cgcacgctgg aggatgtcgc ccttctcctc gacgtgctcg cagggcacga tccgagggat
     1261 ccggatacgc gcccggtggc cacgccgaac ttccgcgccg ttgccgctga gccgccgcca
     1321 cttccgccga aattcgctct ggtgaaaaca cccgtctggg agaaggcgga cgcatccacc
     1381 cgcgagatct tcgaagatct catcagcagc ctgggtgaga acggcggggt agcggagctg
     1441 ccggaggcct tctccgaagc ctggcaggcg catcagacca tcatgtcgat cgatatggcc
     1501 tatcggctcg gcggcgctgt cgatcgcgga agcaaggagg catccagtca acggcttcgg
     1561 gacattatcg ccgagggccg tcagaggttg gctatcgact atctggctgc ccacgatttg
     1621 cggccgcgtc tcgagtccgg tctgtcccca ctcttcgact actacgacgc gatcatcacg
     1681 ccggccagtg tcggcagcgc gccgaagtcg ctggactcga caggcgatcc ggtattctgc
     1741 acgctatgga cgctgctcgg cgtgccggcc ctgtctctac cggtgctgca ggcgcccgac
     1801 ggcatgccgc tcggacttca gcttgtcgga ccagccaacg acgacgcgcg cctgctgcgt
     1861 tcggcgcgcg cgctcatcga aatgctttcg aaagacgaag gctgagagtg cgctgaaggg
     1921 ctgccgctct cagtgccagt ttcgcggccc gcgaagcagg ggcgcgaatg cgtcctgcca
     1981 gaatgcgatc gccatcagga tgaaggcgag aatcgagacg atccacaacg ggatttcggc
     2041 caccgtcact acgattgcca cgacgaagcc aatcatcagc accattccga tgatcgccga
     2101 aactgcgtcc gccattgtgt ctcacctcgc cccttaggct tgcgttccgc ttcgctcata
     2161 cccgatgatg atattgcatc gccctgctgg agcaaagcca attgcgccgc gtgacggacg
     2221 attgaggtgg acgcgagctg gtccgcgttt cccgttccag tctctacacg agggggacaa
     2281 gcgatgattg cagtcatttt cgaagtgaca cccgccgagg ggcgctggca ggagtatctc
     2341 gacctcgccg ccgaactcaa acctcagctc gagaagatgg atggtttcat ctcggtcgag
     2401 cggtttcaga gcctctcaga tccgaaaaaa atcctgtccc tctccatctg gcgagacgaa
     2461 gaggccgtcg cccgctggcg caattatcca gcccatcgcc gcacacaggc gaaggggcgc
     2521 gcgggcgtgt tcgcgggata ccggctccgg gtcgccagca tcatccggga ctacgaaatg
     2581 acggagcgcc gggaggaggc tccaactgac agcaaagcgg tgttcgacgc caagggacag
     2641 tgagccccgg tcctggccgc ctatgacgga gacaggccgc gcattgcagt gcggcctctc
     2701 ttaggttagg gcgtctacct gccgcctcaa cgtgcaaagg cggaactggc ctgtgcggcg
     2761 ggtctcctcg atggcggcgg agccttgccg gcgagagccc tggcataggc ccgctcaaga
     2821 ttgcggcagc ggctgcggga tgaaggatgg gtcgctgtcc gcggcatgtt gccgaaggag
     2881 cggcagacct gctgcagccc cgctcgatcg agccagccct ctctgacccc gcggttaacc
     2941 gcccacgaat ctgctccgag ctcgctggcc ccaacgctgt ggtggccgca ctcgtgataa
     3001 tagacgaaga ggcgcaccgt cggaggcatt cggttgagca gccgaggatt gatgaccagg
     3061 accccagggg cggctgcgcc ctcgctggga agacctccgt cgagcacatg acgtacgttg
     3121 ccgcagcgca ggttgcgacc tgcgatgctc atcgtaccat cgcgctgtac tttgagagaa
     3181 ttcgatgccg gcgccgcctg gccggccgaa gctagactga acagggctgc aacggccgtc
     3241 gccagtccga gtagccgaca aatagtcttc accggatccc ccgtactcac actccacgag
     3301 actcttccct tatgttgcat acgcatttgc gccgaaattg cggcaagtcg gccgtgcaag
     3361 tgcaggttga tcgaacgcgg gggaacggca gttcggctgg cgtgttgatg atgatcaatg
     3421 cccggtgcgc agattgctga gcatctgggg caaggaacag caccagatca gggaggcagc
     3481 gatatgaagg ctcacgaaat catgagtcgg gacgttgtca cggtcaaacc agcaacgccc
     3541 gttcgcgacg tcgccgcgct catgaccgag aagcggatca gcggaattcc ggtcgtctcg
     3601 gaggacggca cggtgctcgg aatcatcagc gagagcgacc tgttgcggcg cgccgagatc
     3661 ggcaccgtgc cgcaacgaaa gtggtggctg gttttcttca gcgatccgga cgcgctggct
     3721 cgccagtaca ccaaaacgca cggcctcagg gccgagaacg tcatgacgcg tcccgtcatt
     3781 tcggtcggag aggatgcgga cctgagcgag atcgcgcgca cgctggacaa acacaagatc
     3841 aagcgcctgc cggtgctgcg cgacggcaag ctcgtcggga tcatatcccg ggcagacatc
     3901 gtacgggcgt tgagtcaggc ggaagtcgag cggcggccga tgagcgacgc agcgctggag
     3961 aaaacgctgc tggagaaaat gcgcgcacag gattggctcg aggacacatt catcaatctg
     4021 agcgtgcacg acggcgtcgt agaattgaac ggcttcgtcg cgtccgccga acagcgcagg
     4081 gctctccacg tcctcattga ggaaacggat ggcgtgcaca aaatcgacga ccggctttcc
     4141 atcgggctac cagcgctgcg tgcgacctaa atcagcgctc ccaaatgcac ttgcttcgct
     4201 aaggccgagg gcgatcgggc aaaccgaatg gctcgatcgc ccacccttgt tccgcctcca
     4261 ctgatcaaaa aaaatcgcac cctgcaatca cgtgttccgc gcgtgacagc gctctgcgcg
     4321 ctcatccggc attctcatca tatcggcgga cggtaaagcc gcagccgagg agccccgacc
     4381 gggagatcat gagcacttcc ggggcgctta accgaaagca ggagttgaga tatgagcaag
     4441 acaatgatca ttggactgac tgcagggctg atggcgcttt ccgcactgca ggcacccgct
     4501 gccgaagcca aaggcaaggg cttctaccac cataagcacg gcaagcacca cggcttcgta
     4561 tggcacaagt ggcacaagcc gcacttcgtc cactacgacg gctgtggctt ctacttctac
     4621 aaatggaagc acaccggcaa gcacttctgg aagacgaagt acttcgtgtg caagggcttc
     4681 tactgacgga aacttcgcct cggatgtccc cctgcacccc tgccgagtcg aagttgtccg
     4741 agcccctcag cccaggctga ggggcccttt tgcgatctca ggctcggcgg gaaacatcat
     4801 ggaaatgcgc aagcccagca gccgcgccta ctccacgctc tgcgtgtcga tatgcggcat
     4861 ccccacgacc ttgcccaccg gaacggtgaa cgcgagccca cgccctggct catcgagttc
     4921 ggcagcctga atgatttccg cgaggatagc ctcagcctca tcctcccgca cgagcgtgaa
     4981 aatgatctcc ttttccggct cgatgggaat gccgaaaatc gtatccttct cgttgacgcc
     5041 gatgccgcgt ccgaaaatga cagtgccgcc ggtcgcgccg gcctttatcg aggcttcaag
     5101 aacgggctgc ccccatcctt tgcgcacaat ggtcacgatc agagtgggat tgcgcatgtt
     5161 tacacctttg cggacgcttt ccaccggatg agaaggccga gcagcatcac ggataatatg
     5221 ggcgccaacg caatgagcgc cacaaggccc agcccttgca ccatcacatc ttgagtacca
     5281 ttggatgctg atgcccctaa cgcgagggcc agcaaaaagg tattggccaa cggccccgtg
     5341 gccactccgc cggaatcgat ggcgatcgct acgaactcct tgctgctgag ccaggtcagg
     5401 atgaccgcca gcgcatagcc cggaaccagc agatacaaaa ttgaaatttc gtacacgatc
     5461 cgcagtagtc ccaaggccac ggagacggaa acgccaatgc agatggtgct gacgacgaga
     5521 gattgcggaa tggagccgtt ggaagcgtgt tcaatctctc ctgcgagaat acgaaccgcc
     5581 ggctcacccc acgtggtcac aaatcccagg aacaagccga ccgcaagaag aacccacggc
     5641 tcctgcatcc cgccgagggc ggcgccgatc acgcgcccaa acggtaggaa gccaattccc
     5701 acgccaagca ggaagagaaa caatcccgcg gtggcaaaca gcgtcccgat cagaatgcct
     5761 cggatctgct tgcgtggcag accgagcaga aagagctgga acgctacgaa aagcgcggcg
     5821 agcggcaaca cggccatgac gacgctccag gccgtgtccg tcagctcgct cagcagggat
     5881 ataccggtca ttggatcgcg agtcccataa tcatgaccgc gataatggct ccgatcgagg
     5941 caaagcccaa aagaccgaac ccgtctgaca ccgtggaccg gccggcgagc accgacgtca
     6001 agccgatggc tatggagatg actacgggcg tggtcagaac tcccgttgtg acactcccgg
     6061 cgtcgtaggc catcggcacg aacttctccg gtgtgacgta cgaaagagcg agcacggcta
     6121 tgagcgaagc cgtgagcaaa ttgcggatgg agatgtcgta gatgatcctc gccatggcca
     6181 gcgcaacgaa agtcgccact ccaagcgccg tgacagcgag gacgatcaac cgattgatgt
     6241 ccccgcgcga gatctcatcg acctgcgcag caagcaccaa tacgtccggc tcggcaaccg
     6301 tcgtggcaaa gcccatggcg aagccgacca gtacgatgag agccaccgag ccttttcttg
     6361 gcagctcagc cccgacgaat ctgcccatgg gcagaatgcc ataatcgacg ccgaggaaga
     6421 gcaggaacat cccggcgatg atcagcaccg acccggccag aaattgaaca aagacggcag
     6481 cgggtgcctg cacgagtgtg aactgcagca ggcacaccgc gccgatgagc ggcgccacgg
     6541 tttgtagcac atcgacaagt cgtcgtttca gttccgacca catgatcctg ccacgagtag
     6601 ttccagctcg atagcgacat tagccaaagg aacgaggctt gaggtcaaat gaattacgct
     6661 gctagcctga tggtgcggca ggcaatccac aactcatatc cgcaggcccg cgagcttccg
     6721 gtcgcacgct ggaaccagat cccgcttacg gcttacaacg ccagacttga tcgatccagg
     6781 cagggtgtgc ccagccccaa cccaatcatt cccactcgat cgtgccgggc ggcttggagg
     6841 tgatgtcgta gacaacgcga ttgatgcctt gcacctcgtt gatgatccgc gttgccgtgc
     6901 gtccaaggaa atcgtgaggg aatgggtagt agtcagcggt catgccatcg gtggaggtca
     6961 cggccctgag cgcgcacacg tggtcgtagg tgcgcgcgtc gcccatgacg ccaacggtac
     7021 gcaccggcag caggaccgcg aatgcctgcc agatcgtgtc gtaaagcccg gccttgcgga
     7081 tctcctccag gtagatcgcg tcggcacgcc gcaaaatatc gagcttctcg cgtgtgacct
     7141 ctcccggcac acgaatggcg aggcccggac ctgga
//
LOCUS       DASVDU010000005        35844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_177643, whole genome shotgun sequence.
ACCESSION   DASVDU010000005 DASVDU010000000
VERSION     DASVDU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 35844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35844
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177643"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1121
                     /locus_tag="VF226_00185"
     CDS             270..1121
                     /locus_tag="VF226_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX7072436.1"
                     /translation="MISRQGQETTSGTGMLAEDRARAIVDLVRREGFQAIETLAGQFG
                     VTPQTIRRDVNLLCDRGLLRRRHGGVELPPDGENLAYPARQVLNIEAKRRIARLVAGA
                     IPEGASLFFGIGTTPEQCALALTDHPGLRVMTNNLNAAIALSRNPSCEITVAGGRLRN
                     LDRDMIAGEARDFFSRFMVDIGIYGVGGVAEDGTLLDFSSDEVHMRRSLAQHCRQRFL
                     VLDHSKFGRGATVRGGHIAEASAVFTDQPVPAPIAARLQDAGVRLVVWSEQHSTESPQ
                     QHNSRRG"
     gene            1124..2146
                     /locus_tag="VF226_00190"
     CDS             1124..2146
                     /locus_tag="VF226_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7072437.1"
                     /translation="MFRRTFLAAGMGLGLGIAAFGGIHDANAQSRAICYNCPPQWADW
                     GSQLKAIQAKLGIQVPPDNKNSGQAIAALIAEKANPVADVTYLGGIAADPAKDAGVLA
                     PYKPKNWDKIPADLKDSEGYWFAIHSGTLGLFVNKAALGDKPVPKSWADLLKPQYKGM
                     VGYLDPTSAAVGQLGVMAVNLALGGTYDNLDPAIKYFKELAKNDPIVPKQTSYARVIS
                     GEIPILMDYDFNAYRGQYVDKAPVEFVIPKEGSLTFPYVMGLVNNAPHADAAKKVLDF
                     VLSDQSQAMWGNAFLRPVFADKLSAEAKSKFLPESEYAHAKAVDLKKLAGAQKAIVDR
                     YGADVH"
     gene            2354..3094
                     /locus_tag="VF226_00195"
     CDS             2354..3094
                     /locus_tag="VF226_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072438.1"
                     /translation="MVRLFVIGGSGALGWAAYASIVTNANYFSSLLATLGVAAITTMA
                     TLIISGITGVFLQRHKFFGNAALVAMLTFPLAFPGVVIGFMVIMLAGRQGLIGNISQM
                     LGADKLVFAYSMAGLFTGYVYFSIPRVILTVMAATEKLDPKLEEAARSLGASPLRVAL
                     DVIIPGLKPAFLSSGAICFATAVGAFGTAFTLATRINVLPMTIYTEFTLSANIAMAAA
                     LSFVLGAVTWAVLALARTAAGSSVAAAG"
     gene            3107..3919
                     /locus_tag="VF226_00200"
     CDS             3107..3919
                     /locus_tag="VF226_00200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072439.1"
                     /translation="MTKKRGVLFWLQLGFTLLVCSFLIVPVILSILAGITNNYFLGIS
                     SGFTLRWVLQVWTGYSDTIFRSIWIALACLVCTLVLGVPAAYVLAKRQNALARIIEEL
                     MVMPVAIPGLATALALIVAYGGLAGFRTSWTFILVGHVLFTLPFMVRSVLAVLSAVDL
                     QTLEEGAASLGAGFWTRFFTVVLPNCRSGILAGALMVVTLSVGEFNMTWLLHTPLTKT
                     LPVGLADAYASLRLEVGSAYTLVFFLMIVPLLIALQWFSRPRHQLHAKPQSE"
     gene            3919..4962
                     /locus_tag="VF226_00205"
     CDS             3919..4962
                     /locus_tag="VF226_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072440.1"
                     /translation="MTAIQNPLAIRLESCAKTFPDGTRALEPLHLEIAAGETIVFLGP
                     SGCGKTTTLRIIAGLEEPDAGGRVLFDGQDVTRLAIEKRNVGMVFQSYALFPNMTVAE
                     NIGYGLRIRKLPAADIRARLTEMLAMMRIEKLADRRIDQLSGGQRQRVALARALAVRP
                     RALLLDEPLTALDAKLRDTLRLEIDTLLRSLGITAIYVTHDQAEAMALGDRIVVMEHG
                     RVAQVGHPRDIYRRPATRFVADFIGTMNRLSGRIEGGVFACGAGRFAWAGAPADACEV
                     LFRPEDIRITAQQSAPLRGTVAAAFFLGDRTRLFVEVGEARPLVVESSERRDFSHGET
                     VGLEIDAQNLLVL"
     gene            4971..5789
                     /locus_tag="VF226_00210"
     CDS             4971..5789
                     /locus_tag="VF226_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEX7072441.1"
                     /translation="MLIAQITDTHIKLPGKLAYRKVDTAAMLRACVAELQTLKQQPDL
                     ILLTGDLVDLGRPEEYAHLKEILAPLRQRVIAIPGNHDDRDSMRAAFAQEGYLPADGF
                     LQFAIDDEYPLRIVGLDTVIPGEGGGKLCAERLHWLDETLKRNPDAPTLVMMHHPPFI
                     TGIGHMDKIGLEGRDGFTEIMARHQQVRLILCGHLHRVIHASVGGRPALTCPSPAHQV
                     ALDIDENAPSRFRMEPPGFMLHWWNGGQLITHTAVIGDYEGPYPFFDADGRLID"
     gene            6104..6613
                     /locus_tag="VF226_00215"
     CDS             6104..6613
                     /locus_tag="VF226_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEX7072442.1"
                     /translation="MTISAYLAGPDVFAPDAEDRLARMGRLCRRHGVTPITPIDGTVP
                     PNLVGLSLARHIKAANLDRIRQASCVIANISPFRGPNMDPGTAYEIGFAEALGKPVFL
                     WSETDQSLRERTSTGVGDVDDNGWVIENFGLAENLMISVNGTAPLATSLRAALINLDA
                     YFRRLQHPH"
     gene            complement(6923..8380)
                     /locus_tag="VF226_00220"
     CDS             complement(6923..8380)
                     /locus_tag="VF226_00220"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007536313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7072443.1"
                     /translation="MTHLQHADLLRRDAYIGGAWVSADSGKSFAVSNPADGSVIGEVP
                     DLGVGETRRAIAAAEAAWPGWASLTARQRAGILRRWYELMLQHQDDLGALMTLEQGKP
                     LAEAKGEVAYGASFVEWFAEEGRRLYGDVIPSHAPDKRIVVVKQPVGVVAAITPWNFP
                     IAMITRKVAPALAAGCPVVVKPAEDTPLCALALARLAEEAGLPKGVFNVVTTLQAAAV
                     GLELTTNPAVRKVSFTGSTEVGKILMRQGADTVKKLSLELGGNAPFIVFDDADLDAAV
                     AGAMISKYRNTGQTCVCANRLLVQDKIYDAFAAKLTEAVKALKTGPGFAEGVSQGPLI
                     NPDALAKVERLVADAKAKGAKLLLGGARHALGGTFYQPSVITEATPEMACAQEEIFGP
                     VAVLFRFKDEAEAVKLANDTPYGLAAYFYSRDIGRVWRVAEQLQYGIVGINEGLISTA
                     EAPFGGMKESGIGREGSRYGIDGFVEIKYLCFGGV"
     gene            complement(8384..9658)
                     /locus_tag="VF226_00225"
     CDS             complement(8384..9658)
                     /locus_tag="VF226_00225"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX7072444.1"
                     /translation="MGNAEIQLRKSRAIARGVGVLTQLFVDRAENAEIWDVEGNRYID
                     FAAGIAVLNTGHRHPRVMQAVHEQIDRFTHTCHQVVPYESYVRLAERLNAIVPGPDEK
                     KTIFVTTGAEALENAVKIARAATGRNAIVAFSGGFHGRTFMGMAMTGKVLPYKAGFGS
                     MPGDVYHVPFPVELHGVTEDMALSALDKLFKADVDPNRVAAVVIEPVQGEGGFYVAPP
                     AFMKRLKGVCDQYGILLIVDEVQTGFARTGTMLAIEQYDVVPDLVTMAKSLAGGFPLA
                     AVTGRASVMDAPNPGGLGGTYGGNPLGIAAAHAVLDVIQEEELCARANRLGARLKQRL
                     AGLRPDIPQISEIRGPGFMNAVEFADPSTRAPDPAFAERVRQIALAKGLILLTCGIYG
                     NVIRFLSPLTIPDEILGEALELLESAIREARG"
     gene            9848..10651
                     /locus_tag="VF226_00230"
     CDS             9848..10651
                     /locus_tag="VF226_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7072445.1"
                     /translation="MAAIGSEVQMSFSHATFAKWNGGIAKLLESESNGVSLLEQAIHD
                     VIDFDILMIFKYTANHAECLHHNIADARSKIVIDDYLKGPFVLDPFYSHALMERKSSF
                     GAMRDLSPDHFHKSEFYRQHYHLTNIADEIGIFFGLDPAVTAVLSITREKSQRLFSRA
                     EKTLFAAMAPVIETLGRKIWQSDRRARPDALESRMESAFAEFGKRVLSRREMEVATLI
                     LRGHSSLSIGNILDISAGTVKIHRKNIYAKLNISSQAELFHRFLQLFPS"
     gene            complement(10750..11685)
                     /locus_tag="VF226_00235"
     CDS             complement(10750..11685)
                     /locus_tag="VF226_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEX7072446.1"
                     /translation="MNGRIVLALFSRRHDLSFLKPLLERADPLLDVVVWPDARFREAE
                     VAVGWEVPPGMYDEMPRLRLVHSIAAGVDNIIAGQDLHHAQVCRVVDPQQAEGMLQFV
                     LWTVLLYHRRMDAVLANQSSCRWDRPPQLPAADCRVGLMGLGEMGNRIAAVLPMLGYT
                     VNGWSRSARKREGVTVFRGEAGLAPFLASTDVLVCLLPLTDDTRGILCKRVFDALPRG
                     AALVHCGRGEHLVEPDLVQALASGHLRGAVIDVFEDEPLPSGHPFWTLPGVIVTPHMA
                     TMADPNTVAAQIVHNVALLRRGERLCNTVDLKRGY"
     gene            complement(11700..12731)
                     /locus_tag="VF226_00240"
     CDS             complement(11700..12731)
                     /locus_tag="VF226_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7072447.1"
                     /translation="MTAGRLQPLPSAVAIHEVAPRDGLQAERTFLPTPQKIALIDALS
                     RTGVAKIEATSFTSPRAIPALADGEAVMAGISRLPGVVYAALVPNARGCERALAAGVD
                     EVNIVMSCSESHNRSNLRMERSASLDQISAIHSLAMGAAIVNVSLSTAFGCPYEGPVQ
                     AAEVLRLIERIGRFGIGQITLCDTTGMAQPAQVESLFLVALREFPDFRFTAHFHNTRG
                     MGLANCLAALMAGVRHFDASLGGLGGCPFAPGATGNVVNEDLVHMLDAMGIETGVDLD
                     ALIDVARQLPALLGHDVPGQVMKAGRYDRQTAAAPDAAPSRRNVASGPVSPPSNPAFA
                     AMAADSDRH"
     gene            complement(12728..13930)
                     /locus_tag="VF226_00245"
     CDS             complement(12728..13930)
                     /locus_tag="VF226_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7072448.1"
                     /translation="MTVDPAAALAGLRVLELGSLIAGPYAGTLLAQFGAEVIKVEPPG
                     RGDPLRKWRKTYAGTSLWWYSQSRNKKSVAIDLRTPEGGALVREIAASADIVIENFRP
                     GTLEKWGLGWDVLSAINPRLIMLRISGYGQDGPYAGRPGFAAIAECMGGLRHVTGFPD
                     RPPVRSGVSLGDTLASLYGTIGTLIALHHARSRGVGQVVDVALYEAVFAIMESLMPEY
                     SVLGHVRERSGASLPGIAPSNTYVCRDGKYVAIAGNGDSIFQRLMQVIGRADLAARAD
                     LADNEGRVKHAGELDAAIQAWALTLDAPAVIETLNAASVPVGAIYTASDIAADPHFRA
                     RGVLEAAQLPDGTPMTLPGAVPRLCATPGRTSWIGPRLGEHTCDVLAALGVGEERMND
                     LKEKGVIA"
     gene            complement(13927..14499)
                     /locus_tag="VF226_00250"
     CDS             complement(13927..14499)
                     /locus_tag="VF226_00250"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7072449.1"
                     /translation="MAIRSILKMGNPALTVVSEPVADPCAPEIGALIDDMRETLDSVA
                     GIGLAAPQIGVPLRVVLYCLPASRIPAGARTRPVPWTPLINPQITPLTAETVDLWERC
                     LSLPGLYARVPRHPHIHIRWQSADGEWHERECRGYHATLLQHECDHLDGILYPARMPD
                     ARELAFASEVCGPTGVYTYSPAEFDGESAE"
     gene            complement(14524..15561)
                     /locus_tag="VF226_00255"
     CDS             complement(14524..15561)
                     /locus_tag="VF226_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072450.1"
                     /translation="MFPHPRLARAADNLVREQFCVAPGESVLLTADSAVEHALIDTVV
                     QAVVRQGAKPLVALAPSLPFQGGLSDPYVGDPLAAAVVASDVWLDFCFPYHAGSRIHD
                     AAMQGRRCRYGLIALSRADSLSRLYGAVKFDALLDFNIAFMEFVAGAAGQTVRFTCAA
                     GTDVSFVLDEVKLKRSRSMRTPGMDTVPGAQSLYPVKDSVRGRIVIQALFDESYRRLR
                     KPILIEADGGIKSIKGAAPEDAASFERALRRASGKNTLGTFVHFTYGFHPAARMTGEQ
                     FIEDIRLPGTNAIGMGLPWWEAGGGENHPDGVVFDQSLWVGGVPVLDQGHFVGPDPLR
                     RLYDAMTSRTD"
     gene            complement(15599..16621)
                     /locus_tag="VF226_00260"
     CDS             complement(15599..16621)
                     /locus_tag="VF226_00260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX7072451.1"
                     /translation="MPTADGVSMDADIQQAPVAVSGAAARAQVEDIRRLSDDIVEIAL
                     AVPEDCWRPVEAGAHLRLDFGAGLVRCYSLCNIGDVPGRYVVAVKREADSRGGSAAAH
                     ALRIGDALAVAGPFNQFAPDWTEHRLVLVAGGIGITPLYAMAQAAAARGHDFVLHYFL
                     REPRQAAYLDALSAGAYAPHLRVHAGLGPEEVQAAVAALLTEVTRDDGLYVCGPRPLI
                     ELVRAQAADVLPAARIHWESFGGNGDAAAAADADAPFEISLLSGEGPFTVPPGQSALA
                     VLLAAGIDVPFSCREGECGMCVVEVIEGQPDHRDRFLSEDMRSRGKHIALCVSRARGP
                     RIVVDF"
     gene            complement(16608..17762)
                     /locus_tag="VF226_00265"
     CDS             complement(16608..17762)
                     /locus_tag="VF226_00265"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7072452.1"
                     /translation="MTTPTRPSHYVPRGDIAINKTELARSRHLSGDIYTSPEIYAREM
                     EEYFSKEWLFMGRAEQFEKPGDFQARRVLGRPVLIAKNRDGAIQAFHNMCRHRGVEVA
                     DGTGNARSFKCPYHGWTYDLNGQLVGAAYMSDTEGFEPGACRLPPIHLDIWRGNIFIN
                     FAEQPKPFTEAVADLERDFAMLHSERVRLADVTTTTLQCNWKFFHENLMDYYHVGVLH
                     AKTFGQRFSWTPENLVLKPGGGLTMRYLASPSTPDGVSLFGKAPWLEHEENSFACTAF
                     YPPNMTLFGRIDMVKIITAWPTGPDSCEVMIYMMFPEEFFSHENFEEKVGVYREYQKA
                     IYDEDRSMIESMQRAMALPNYEPGRLSVMEKPIHHFLGSYVDRMFGDANG"
     gene            17951..18658
                     /locus_tag="VF226_00270"
     CDS             17951..18658
                     /locus_tag="VF226_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7072453.1"
                     /translation="MNAVLSVSDRKSFSKKAPGVRRQELIDATFRCLRDCGVAETSVR
                     TIAAEAGLSLGMVRHYFNSKDELLAATYRDMSERLKEQTQEILRRTPPDPLSQLVAFI
                     NAGLQPPLLDRSYVRIRFLFFELTHTNPVVKRVHEEIYSRFERQLLTLVTAVAKHYGR
                     DADCRMITRTITAYLKGIWAEWTLSNDDFEPRELLAQLMPFWLQQLAPAAALKAVLPA
                     KAPTNAVRRRTRSAAAS"
     gene            complement(18661..19371)
                     /locus_tag="VF226_00275"
     CDS             complement(18661..19371)
                     /locus_tag="VF226_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7072454.1"
                     /translation="MSTEAQTRDELAALYRLVAHFRMTDVIDTHISARVPGTEHFLIN
                     EYGRLFHEIGPDQLVKIDIHGDIVDPGREGHKRVNRAGFVIHSAIHMARTDVACVVHT
                     HTADGIAVACQKAGLLPISQHALKFYGRLSYHDYEGIAVDLEERERLIRDLGRNEAMI
                     LRNHGLLVTGTSIAAAFTNIYFLERACQVQIKAQAGNSPLHLPSQEVLDRTAAQFDPA
                     SSDWHSQLFWQSARRLLA"
     gene            complement(19368..21032)
                     /locus_tag="VF226_00280"
     CDS             complement(19368..21032)
                     /locus_tag="VF226_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072455.1"
                     /translation="MTTGAAPLLKVRNLRIALPAAGDRLFAVDGLSFDVHPNEIVCLV
                     GESGSGKSMTAHAILGLLPPQVRIDAGSEIVCDGTDLLQLGEQGLQQWRGNRIGMVFQ
                     EPMSALNPLQRIGAQIAEALILHARTLGAEQVRQRVLASLAAVGLPEPERICRAYPFQ
                     LSGGQRQRVMIAMALINQPRVLLADEPTTALDVTTQKQILDLIRRLQRERHMGVLFIT
                     HDIGVVADIADRVIVMRQGCAVEAGTRDEVMLKPRAEYTRALIDALPGRRPATQDEAG
                     GSREVLLQIRGLSKRFVTGGSLWSPAREVAAACDIDLDVREGETVAIVGESGSGKSTL
                     GRMIMRLVEPDQGEILFAGRDILKLPRRDLANFRRDAQLIFQDPFSSLNPRQKVGEAI
                     ARGLIIHGEAPKAARNRARLLLDRVGLGAWAAERFPHEFSGGQRQRVSIARALALQPR
                     LLIADEAVSALDVSIQAQILHLLGDLKREFNLTLLFITHDLRVAGEIADRVVVMSQGR
                     IVETGTPAEVLGAPRHPYTRQLVSAVAGTDFLGQLAQDRAYIEALA"
     gene            complement(21029..21859)
                     /locus_tag="VF226_00285"
     CDS             complement(21029..21859)
                     /locus_tag="VF226_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072456.1"
                     /translation="MKPALLAFLRRPSGVIGLVILVFVVGAALFASAIYPGNPWDTVA
                     TPFTPPGTDGLLMGSDSLGRDVAAGVVHGARISLLIGVTSTFMALVVGVLVGAIAGYS
                     GGRTSSLLMGVTELFQSIPAFILAILLVAILTPSVATITFAIAIVSWPQVARLTRGEF
                     LSLKEREYVQAARCLGESPVSLMVRYILPNALSPVVVAGSLSIATAILIESALSFMGL
                     GDPNMMSWGYMVGTARTMIRQAWWMSFFPGLAILLTVLAINLVGESLNDVLNPRISRQ
                     "
     gene            complement(21865..22872)
                     /locus_tag="VF226_00290"
     CDS             complement(21865..22872)
                     /locus_tag="VF226_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072457.1"
                     /translation="MTNWSDKTLLSVAMLKPVLARVAKMVGVIIAVAIFNFFLVHAAP
                     GDPALVIAGQAGATDEAYMARVRAAYGLDQPLTTQLTGYLRKVVTLDLGYSYRQQRPV
                     AEVIAERLGPTILLTGTAFIISLVGGIVLGGLAGVRAGKFSDTVITVLSLVVYATPVF
                     WLGIMLVLLFAVQLEWLPPFGYQTVGADLAGWALWLDRGRYLLLPAATLGLLYLAIYA
                     RLMRASIIEVSHQDFVKTARAKGAGRSRVLWRHMLRNALLPVVTMAGVQAGTLIGGSV
                     VIETIFAWPGLGRLTYEAVLQRDYQLLLGIFLVMSIVVAIFNLVTDLLYRVVDPRIRS
                     V"
     gene            complement(22915..24510)
                     /locus_tag="VF226_00295"
     CDS             complement(22915..24510)
                     /locus_tag="VF226_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072458.1"
                     /translation="MMVTKRQFNKLLLSAGAICFADLSFGSTRAYGQTRGGTLNAVLQ
                     PEPPMLNIAVNQQTPTQTIAGKIFLSLLTYDFDLKPLPSLAKSWSISADGKTYTFKLE
                     QNVKWHDGQPLTADDVVYTVTEFLPVTNPRAKAIFARCESVTAPDPHTVVFKLKQPFA
                     AFLGAFDIATLPVMPKHIYAGTDLQKNPANFKPIGSGPYKFKEWVRGSHIHLVRNEDY
                     FKPGKPYLDAIIYRIVPDGASRAVAIEEGQVQLTQWTDLELFDAQRLAKNPKFVMTTK
                     GYEYFAPIMYLEMNVRSAPLNDKRFRYAISHAIDREFVRDKILYGFGKVATGPISSKT
                     RFYDGNVRKFDYSIAKATALLDEMGLKPDSEGVRARLKLPVAPYGEMPARACEYIRQS
                     LAKIGIAVTLETSDAAGWAQKIANWDYDLNLTWPFQYGDPAIGVTRLYVSSNIHKVLF
                     SNTIGYSNPEVDRLADAAAAEMDEGKRQALYSQMQKILVEDSPIAWLVEMEFPTIYDK
                     RLQNVVTTGIGIHESFDEVYFKG"
     gene            complement(24580..25590)
                     /locus_tag="VF226_00300"
     CDS             complement(24580..25590)
                     /locus_tag="VF226_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7072459.1"
                     /translation="MHPASTLQLLPVPRELAAFGMHVLCAASRYPRNDSALIMEKVAM
                     DLGAHIRHAREVLGYEKIILAGWSGGGSLSLFYQSQAERPDIVATPAQDPVDLVGANL
                     IAADAVVCQAAHLSRAVMLRDMIDPSVRDESNPDDRDVEFDLYDPANPNKPPYGSDYI
                     AEYRKRQLARVRRITAWVWETLHRLQRRNTGELERGFVTHRTLADPRYLDPAIEPNGR
                     PPGWCYLGNPETVNSGPVGLARFSTLRSWLSQWSIDDSRVDGLACAARISVPLLVIEN
                     EADDCVPQPHARMLFEAGGSADKSFRLIKGASHFYAGQPELMREAALFTRQWLLERNL
                     LN"
     gene            complement(26393..26989)
                     /locus_tag="VF226_00305"
     CDS             complement(26393..26989)
                     /locus_tag="VF226_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7072460.1"
                     /translation="MDPVASLGNEKIATICAAIQGAVIERRLVPGLKLSEESLAGLFG
                     CSRTIIRHAFQRLSKDRIITLLPNRGAFVSEPSVQEAHQIFDARKVVEDHTAALAAAK
                     ATRASIAKLRKLEQDERRAIGSGNRAAALRLSGELHLAIAEIAANAPMYDFLSELISR
                     TSLILALYGAAAKRPAAGMSTSTSSMPLPLETEGAPAA"
     gene            27032..27946
                     /locus_tag="VF226_00310"
     CDS             27032..27946
                     /locus_tag="VF226_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7072461.1"
                     /translation="MAGVEGIPEDEALDLMREILQFATQPQYVYQHRWAAGDMLSGTI
                     DARCTRAANTTPSTTAAGCTAAPLREQPHPASDMPTLLFYNPNSSTSVTEKILEVARR
                     NVPDDTEVVGYTSPKGPSYIGSAPDLLEAAVAALDWVKTEAAKYDAVVLACFSDTGIA
                     ALRQAAPVPIVGLIETSVMAAQLLGKRFSIVTGGDEWRRLLPPMLNAIGQQSRLASIR
                     ALGQSGLQIMKNPEKACQVLAEATQSAFDDDGADVVILGGAALAGMDTAVQAAVSGMV
                     IDSVAPAAWMAAALARKRPVSILHPPAG"
     gene            complement(28381..28641)
                     /locus_tag="VF226_00315"
     CDS             complement(28381..28641)
                     /locus_tag="VF226_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7072462.1"
                     /translation="METAGIGWIAAIIIGGFAGWLAEKFMKSDMGVFLNILLGIVGAA
                     IASAVLSWFGIVLGGRVGYLVAGFIGACILIWIGRLLKGRRA"
     gene            complement(29106..30326)
                     /locus_tag="VF226_00320"
     CDS             complement(29106..30326)
                     /locus_tag="VF226_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX7072463.1"
                     /translation="MAPISSNLIVRLSDRQVGTITRLAGDRHVFAFDEAYIDDPNRPI
                     LSLSFKSQTGGVVTAIRPHSVRLPPFFSNLLPEGHLRDYLAARAGVKPEREFFLLAAL
                     GEDLPGAVTVHEDGAGDRDHAADHHHDALTNALRFSLAGVQLKFSAVMEAAGGLTIPA
                     GGVGGEWIVKLPSARFPAVPENEFAMMELARRIGIEVPKLGLIPVEAIAGLPDEARAV
                     AGKALVVERFDRTADGGRVHMEDFAQVFGIFPDDKYDKRSYANIAAVLAAEIGEAAVI
                     DFMRRLAFSILIGNADMHLKNWSLLYPDGRKPVLAPAYDYVSTVPYLPKQQLALGFGR
                     SKAMDDITPDQVRRFADTAQLSVPFLWKTILRTVEETRAAWRDHAARDLLPADLRKTL
                     EARMENVAANIGRH"
     gene            complement(30326..30652)
                     /locus_tag="VF226_00325"
     CDS             complement(30326..30652)
                     /locus_tag="VF226_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7072464.1"
                     /translation="MKSLPEGLRTEIRDARLAKDWSQLELGEKVGLGQKHVSGIETGK
                     IVPRFDTLLDLLRALDLDVVVVPRALTPIVSSLAREHRNRAAHGTEDAERPLYASGGE
                     NDEEVA"
     gene            complement(30833..31100)
                     /locus_tag="VF226_00330"
                     /pseudo
     CDS             complement(30833..31100)
                     /locus_tag="VF226_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272610.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            31968..32234
                     /locus_tag="VF226_00335"
     CDS             31968..32234
                     /locus_tag="VF226_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072465.1"
                     /translation="MPIQRAGNPKSRIGGLPRDAARQFFASHNARLLNADKSRLTETE
                     KRIVEARRHNIRIASMSYMSLQENALGVHVGKSASSGWRCNAGM"
     gene            complement(32254..32535)
                     /locus_tag="VF226_00340"
     CDS             complement(32254..32535)
                     /locus_tag="VF226_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015397648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7072466.1"
                     /translation="MREPVHPGEILREEVLAPLDLSVTEAADRLGMSRVALSRVLHGR
                     AAISPDLAIRLERAGVSTACAWLAMQAQYDIAKALRRKQPRVRRLEEAA"
     gene            32744..33070
                     /locus_tag="VF226_00345"
     CDS             32744..33070
                     /locus_tag="VF226_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072467.1"
                     /translation="MIQFCAHVNRRDDMKIGLVTSVSLGSGQRSAEQRIRDMAARYRV
                     KPVRTKLDGWADDVARLSDADVARDEVIDLPDEVIDLVVALQRAGFVTTKEAIDLCGD
                     FLAERQ"
     gene            33393..33734
                     /locus_tag="VF226_00350"
     CDS             33393..33734
                     /locus_tag="VF226_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7072468.1"
                     /translation="MTNTSKITESPPAAVEDALRRLGRNIRTARVRRKLRIEDVAKRI
                     GASRFTVSDLEKGKPGTSAAAYFGALWALGLIEQAADLADPDRDEEGKALESARHPTQ
                     APRRRKLDNDF"
     gene            33724..35001
                     /locus_tag="VF226_00355"
     CDS             33724..35001
                     /locus_tag="VF226_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX7072469.1"
                     /translation="MTSERECYVYIVPPGATEFVTAGRFQVSETRDGLPLGQFVYGRR
                     YLARNDAVELDPVELRLAGRTYETGRMKGVFGAIRDSMPDNWGRRVIEKHSGHTQLEE
                     FDYLMEGADDRAGALGFGLGVEPPAPRRQFNRTLDLDRLQKAADAIIADDPDRAGSAR
                     AQAEELLLEGTSMGGARPKTVVQDDHALWLAKFGRQDDRWNHPRVEHALLLLARACEI
                     DAADSRIETIGGRDVLLVRRFDRDWSGEGYHRHRMVSALTLLQSEDSQADRGRWSYLL
                     LADEVRRASAQPEADLRELFARMCFNAAISNLDDHPRNHALLAKGRAWRLSPAYDLTP
                     NPVVALDRRDLAMDCGPAGRYANKANLIGAAGRFLLGQDEAAAIFARVTETVRGQWHA
                     TMRRAGVNDRDCDAIASAFLYDGLFFEAAGQNV"
     gene            35021..35272
                     /locus_tag="VF226_00360"
     CDS             35021..35272
                     /locus_tag="VF226_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072470.1"
                     /translation="MDIYDWLKLLGAAVGGGVTVKVLDILYQEFRRRSDSSASAKRFV
                     DQHLDPLLKAADEVAGKLRSLAPEAASEHPGNRPASPTG"
     gene            35430..>35844
                     /locus_tag="VF226_00365"
     CDS             35430..>35844
                     /locus_tag="VF226_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072471.1"
                     /translation="MFTAPARPTTIVQPKVAAPWLTFILQIAMRDAVPREPAGPVLQL
                     AGMNVADHAVRLRYGIFGIARPVGLLELDDDDGRQRLAFLAKGKRVVEMRMSGRLPDS
                     LSTAGKALSGRSEDKNEWKVSRSSMIQRFCWANGTS"
BASE COUNT         6890 a        11481 c        11105 g         6368 t
ORIGIN      
        1 gttggacaga aattgcacgg caagagtctc cgttctgggt agccgggcag cggcgcgcaa
       61 cggtcctgca cgcccgggcc taccggatgt ggtcgcaatg gaatgggaat gtcggcgcca
      121 taacgtcggc ggggcggcgg aggaattccg ggggcatgac gcgccagatc cgacagccaa
      181 ttcccgtcaa gcggtttcgt ttgacgacat ataaggttca tgcgaaagtc atattaatga
      241 aataagcggc gatcagtgtt cgaacgaaca tgatcagccg ccaaggacag gaaacgacct
      301 cagggaccgg catgctggcg gaagaccgcg cacgcgccat cgtcgacctt gtgcgccgcg
      361 agggcttcca ggccatcgag acgctggccg gccagttcgg cgtcaccccc cagaccatcc
      421 gccgggacgt caacctgctc tgcgaccgcg gcctgctgcg ccgccgccat ggcggggtcg
      481 agctgccgcc cgacggcgag aatctcgcct atccggcccg ccaggtgctg aacattgagg
      541 ccaagcgccg catcgcccgg ctggtcgccg gcgctatccc cgagggcgcc tcgctgttct
      601 tcggcatcgg caccacgccc gagcaatgcg cgctggcgct gaccgaccat cccggcctgc
      661 gggtgatgac caacaatctc aacgccgcca tcgcgctgag ccgcaatccc tcctgcgaga
      721 tcaccgtggc cggcggtcgc ctgcgcaatc tcgaccgcga catgatcgcc ggcgaggcgc
      781 gcgacttctt cagccgcttc atggtcgata tcggcatcta tggcgtcggc ggcgtcgccg
      841 aggacggcac actcctcgat ttcagcagcg acgaggtgca tatgcgccgc agcctggcgc
      901 agcattgccg ccagcgtttc ctggtgctcg accattccaa attcggccgc ggcgccacgg
      961 tgcgcggcgg gcatatcgcc gaggccagcg ccgtctttac cgaccagccg gtgcccgcgc
     1021 ccattgccgc ccgcctgcag gatgccggcg tgcggctcgt cgtctggtcg gaacagcatt
     1081 ccactgaatc accgcagcag cataacagca ggagaggctg accatgttta gacgtacatt
     1141 ccttgccgca ggtatgggcc tcgggctcgg catcgccgcg ttcggcggta tccacgatgc
     1201 caacgcgcag agccgcgcca tctgctacaa ctgcccgccg caatgggccg actggggttc
     1261 gcagctcaag gcgatccagg ccaagctcgg catccaggtg ccgcccgaca acaagaattc
     1321 cggccaggcc atcgccgccc tgatcgccga gaaggccaat ccggtggccg atgtcaccta
     1381 tctcggcggc atcgccgccg atcccgcgaa agacgccggc gtgctggcgc cctacaagcc
     1441 gaagaactgg gacaagatcc ccgccgacct gaaggattcg gaaggctact ggttcgccat
     1501 ccattcgggc acgctcggcc tgttcgtcaa caaggccgcg ctcggcgaca agccggtgcc
     1561 gaagagctgg gccgatctgc tgaaaccgca atacaagggc atggtcggct atctcgatcc
     1621 gacctcggcc gccgtcggcc agctcggcgt catggcggtg aacctcgcgc tcggcggcac
     1681 ctacgacaac ctcgatccgg cgatcaagta cttcaaggag ctggccaaga acgatccgat
     1741 cgtgccgaag cagacctcct atgcccgcgt catctcgggc gagatcccga tcctgatgga
     1801 ttatgacttc aacgcctatc gcggccagta tgtcgacaag gcaccggtcg agttcgtcat
     1861 tccgaaggag ggcagcctca cctttcccta tgtgatgggt ctggtgaaca acgcgccgca
     1921 tgccgacgca gccaagaaag tgctcgactt cgtgctgtcg gatcagagcc aggcgatgtg
     1981 gggcaatgct ttcctgcgcc cggttttcgc cgacaagctt tcggccgagg caaagtcgaa
     2041 gttcctgccc gaatccgaat acgcccatgc caaggccgtc gacctgaaga agctggccgg
     2101 tgcgcagaag gcgatcgtcg accgctacgg cgcggacgtg cactgaccaa gcggcgcttg
     2161 atgggcaagc gcgccgtctt gacttgagac gaaggaaacc tacggggcga tggacggcca
     2221 tcgccccgct tttccagctt cgcggggagt ggacatggca gcagaatcgg gcatgaggcg
     2281 gcgcgacacc atccgtctgg cggctctatt gctgccggcc ggcatcttct tcatcgcctt
     2341 cttcctgctg cccatggtcc gcttgttcgt gatcggcggc agcggcgcgc tcggctgggc
     2401 cgcctatgcc agtatcgtca ccaatgccaa ctatttcagc tccctgctcg cgaccctcgg
     2461 cgttgccgcc attaccacaa tggccacgct gatcatcagc ggcatcaccg gcgtcttcct
     2521 gcagcgccac aagtttttcg gcaacgccgc gctggtggcg atgctcacct tcccgctcgc
     2581 cttccccggc gtcgtcatcg gcttcatggt catcatgctg gccggccgcc aaggcctgat
     2641 cggcaatatc agccagatgc tgggcgccga caagctggtc ttcgcctatt ccatggcggg
     2701 cctgttcacc ggctatgtct atttctccat cccgcgtgtc attctcaccg tgatggcggc
     2761 gaccgagaaa ctcgacccga agctcgagga ggccgcccgc tcgcttggcg cctcccccct
     2821 gcgcgtcgcg ctcgacgtga tcataccagg attgaagccg gccttcctgt cctcgggtgc
     2881 gatctgcttc gccaccgccg tcggcgcctt cggaacggcg tttacgctgg ccacccgcat
     2941 caacgtgctg ccgatgacga tctacacgga gttcacgctg tcggctaaca tcgccatggc
     3001 ggcggcgctc agcttcgtgc tcggcgcagt gacctgggcc gtgctggcac tggcacgcac
     3061 cgcggccggc agttcggtcg ccgcagcggg ttgagaaggc gcgacaatga caaagaagcg
     3121 cggcgtcctg ttctggttgc agctcggctt caccctgctg gtctgcagct tcctgatcgt
     3181 gccggtgatc ctgtcgattc tcgcgggcat caccaacaat tactttctcg gcatttccag
     3241 cggcttcacc ctgcgctggg tgcttcaggt ctggacgggc tacagcgaca cgatcttccg
     3301 ctcgatctgg atcgccctcg cctgtctcgt ctgcaccctg gtgctcggcg tgccggccgc
     3361 ctatgtgctg gccaagcggc agaacgcgct cgcccgcatc atcgaggagc tgatggtcat
     3421 gccggtcgcg atccccggcc tcgccaccgc gctcgccctg attgtcgcct atggcggcct
     3481 tgcgggcttc cgcacctcct ggaccttcat cctggtcggt catgtgctgt tcacgctgcc
     3541 cttcatggtg cgctcggtac tggcggtgct gagtgccgtc gacctgcaga cgctggagga
     3601 aggcgccgct tcgctcggtg ccggcttctg gacccgcttc ttcaccgtgg tgctgccgaa
     3661 ctgccgctcc ggcattctcg ccggtgcgct gatggtcgtg accctttcgg tcggcgagtt
     3721 caacatgacc tggctgctgc atacgccgct gaccaagacc ctgccggtcg gccttgccga
     3781 cgcctacgcc tcgctacgcc tcgaagtcgg cagcgcctac accctcgtct tcttcctgat
     3841 gattgtgccg ctgctgatcg cgttgcaatg gttcagccgg ccgcgccacc agctgcatgc
     3901 caagccacag agtgagtgat gactgcgatc cagaacccgc tggcgatccg cctcgaatcc
     3961 tgcgccaaga cctttcccga cggcacccgc gcgctggagc cgctccatct cgagatcgcc
     4021 gccggcgaga cgattgtctt cctcggcccc tccggctgcg gcaagaccac cacgctgcgc
     4081 atcatcgccg gccttgagga gcccgatgcc ggtggccgcg tgctgttcga cgggcaggat
     4141 gtcacccggc tcgccatcga gaagcgcaat gtcggcatgg tattccaatc ctacgccctc
     4201 ttccccaaca tgacggtggc ggaaaacatc ggctatggcc tgcgcattcg caaattgccg
     4261 gctgccgaca tccgcgcccg cctcaccgag atgctggcga tgatgcgcat cgagaaattg
     4321 gccgaccgcc gcatcgacca gttgtcgggc ggccagcgcc agcgtgtcgc gctggcccgc
     4381 gcgctcgctg tccggccgcg tgccctgctg ctcgacgagc cgctgaccgc gctggacgcc
     4441 aagctgcgcg acacgctgcg ccttgagatc gataccctgc tgcgcagctt gggcatcacc
     4501 gccatctacg tgacccacga ccaggccgag gcgatggcgc tcggcgaccg cattgtggtg
     4561 atggaacatg gccgtgttgc ccaggtcggc catccgcgcg acatctaccg caggccggcg
     4621 acgcgtttcg tcgccgattt catcggcacc atgaaccgcc tgagcggccg gattgaaggc
     4681 ggcgtcttcg cctgcggcgc cggccgcttc gcctgggccg gcgcgccagc cgacgcctgc
     4741 gaggttctgt tccggcccga ggatatccgc atcaccgcac agcaatcggc gcctttgcgc
     4801 ggcaccgtcg ccgccgcctt cttcctcggc gaccgtacga ggctgttcgt cgaggtcggc
     4861 gaggcgcggc ctttggtcgt cgagagcagc gaaagacgcg atttcagcca tggcgagacg
     4921 gtcggactcg agatcgatgc gcaaaacctt cttgttcttt gaggcccgcc atgctgatcg
     4981 cccagataac cgatacccat atcaagctgc ccggcaagct ggcctacagg aaggtcgaca
     5041 ccgccgcaat gctgcgcgct tgcgtcgccg aactgcagac cctgaaacag cagccagacc
     5101 tgatcctgct gaccggcgat ctggtcgatc tcggccggcc cgaggaatac gctcatctga
     5161 aggagattct cgcgccgctg cggcagcgcg tcatcgccat cccgggcaac catgacgacc
     5221 gcgacagcat gcgcgccgca ttcgcgcagg agggctatct tccggccgat ggcttcctgc
     5281 agttcgcgat cgacgacgag tatccgctgc gcatcgtcgg cctcgacacg gtgatcccgg
     5341 gcgaaggcgg aggcaagctt tgtgccgaac ggctccattg gctggatgag acgctgaagc
     5401 ggaatcctga tgcgccgacc ctggtgatga tgcatcatcc gcccttcatc accggcatcg
     5461 gccatatgga caagatcggc ctcgagggcc gcgacggctt cacagagatc atggctcggc
     5521 atcagcaggt acggctgatc ctgtgcggtc acctgcaccg cgtgatccac gccagtgtcg
     5581 gcggccgacc ggctctgacc tgcccgagcc cggcgcatca ggtggcgctc gacatcgacg
     5641 agaatgcgcc gagccgcttc cgcatggagc caccgggctt catgctgcat tggtggaatg
     5701 gcggccagct catcacccac acggcggtga taggcgatta tgaggggccg tacccgttct
     5761 tcgatgccga cggcaggctg atagactaga gatggctccg ccctttttgg aaggggccgc
     5821 cagtgcatgg ccgtctcccg ccagggaggc cgcaaaggat gcggcggagt tccgatctgc
     5881 ctcaaatccc gtaccgccat cggctccccg gccggccggc gcttggccgc cgagatcgtc
     5941 gcccaggggc attggccagt tctcgccctc catccgccgt cctgacgcag ctcgccgcct
     6001 cccatccgcc tccggtccgg aacgcgcggc cgggcactgc ctggcggctg gaggaagcat
     6061 cctgatcccg agatgacgcg gccatgaggg ctctgcaatg ccattgacca tttccgccta
     6121 tctggcaggt cccgacgttt ttgcgccaga tgcagaagac agactggcca gaatgggccg
     6181 gctttgccgc cggcacggcg tcacgccgat cactccgatc gacggaacgg ttcctccaaa
     6241 tctcgtcggc ttatccctcg cccgccatat caaggccgcc aaccttgatc gcatccggca
     6301 agccagttgc gtcatcgcca acatttcgcc attccgcggt cccaatatgg atcccggcac
     6361 ggcttacgag atcggatttg ccgaagcctt gggtaagccg gtcttcctct ggtccgaaac
     6421 cgaccagtcg cttcgcgagc gcacctcgac cggagttggc gatgtggatg ataatggctg
     6481 ggttattgag aattttggct tagccgaaaa tcttatgatc agcgtgaatg gcaccgcgcc
     6541 tctcgccacc agccttcgtg cagcgctgat caatctcgat gcgtattttc gccgcttgca
     6601 gcatccccat taggatgtct tgaagagacc aagcggaccg gcctcgttgc agcagcaatt
     6661 gcaaagccgt aagccatcgg gatgacggac gtggatgtgg cgcccttgcc agcaaagagc
     6721 gaatccctgg ggatgttttg actgtatgtc caccctggcg gacggatctc aaccttgttc
     6781 gccgccggcc ttgtcctgcc caggccagcg cgcggcgcac cgagcatggg atcgccgtcc
     6841 gctgcatgca gtcaacggcg gagcataaga agcaaggcgc gcggcgcgcc gtatatgacc
     6901 gcgccgcgca gccgtttcat gctcatacgc cgccgaagca gagatacttg atttcgacga
     6961 agccatcgat gccgtatctg gagccctcgc ggccgatgcc ggattccttc atgccgccaa
     7021 acggcgcctc cgccgtcgag atcaggcctt cgttgatgcc gacgatgccg tattgcagct
     7081 gctcggcgac ccgccagacc cggccgatat cgcggctgta gaaataggcc gccaggccat
     7141 agggcgtatc gttggccagc ttgacggcct cggcctcatc cttgaagcgg aacagcaccg
     7201 cgaccggacc gaagatctcc tcctgggcgc aggccatctc gggcgtcgcc tcggtgatca
     7261 cgctgggctg gtagaaggtg ccgcccaatg catgccgcgc gccgccgagc agcagcttgg
     7321 cgcctttcgc cttggcatcc gccaccagcc gctcgacctt ggccagcgca tccgggttga
     7381 tcagcggccc ctggctgacc ccttccgcga agcccggccc ggtcttgagc gccttcacgg
     7441 cttcggtgag cttggcggcg aaggcatcat agatcttgtc ctgcaccagc aggcggttgg
     7501 cgcagacgca ggtctgcccg gtattgcggt atttcgagat catcgccccg gcgacggcgg
     7561 cgtcgagatc ggcatcgtcg aagacgatga agggcgcatt gccgccgagc tcgagcgaca
     7621 gcttcttcac cgtatccgcg ccctggcgca tcaggatctt gcccacctcg gtcgagccgg
     7681 tgaacgagac cttgcgcacg gccggattgg tggtcagctc cagcccgacc gccgccgcct
     7741 gcagggtggt gacaacgttg aacaccccct tcggcaggcc ggcctcctcg gcaagccggg
     7801 ccagggccag cgcgcaaagc ggcgtatcct ccgccggctt gaccaccacc gggcagccgg
     7861 cggccagcgc cggcgccacc ttccgggtga tcatggcgat cgggaagttc cacggcgtga
     7921 tcgccgcaac caccccgacc ggctgcttga ccacgacgat ccgcttgtcc ggcgcatggc
     7981 tggggatgac atcgccataa aggcggcggc cctcctcggc gaaccattcg acgaaactgg
     8041 cgccataggc cacctcgcct ttcgcttccg ccaacggctt gccctgctcc agcgtcatca
     8101 gcgccccgag atcgtcctgg tgctgcagca tcagctcgta ccaccggcgc aggatgcccg
     8161 cgcgctgcct ggccgtcagc gacgcccagc ccggccaggc agcctctgcg gctgcgatcg
     8221 cccgccgcgt ctcgcccaca cccagatccg gcacctcgcc gatcacgctg ccatccgccg
     8281 gattgctgac cgcgaagctc ttgccgctat cggcactcac ccaggcgccg ccgatatagg
     8341 catcacggcg cagaagatcg gcatgctgca aatgcgtcat ggctcagccc cttgcttcgc
     8401 gaatggcgct ctcgaggagc tcgagcgcct cgcccaatat ctcgtcgggg atggtgagcg
     8461 gcgagaggaa gcggatgaca ttcccgtaga tgccgcatgt cagcaggatc agccccttcg
     8521 caagggcgat ttggcggacc cgttcggcaa aagccggatc cggcgcccgc gttgagggat
     8581 cggcaaattc aaccgcattc atgaatcctg gaccgcggat ttccgatatc tgcgggatat
     8641 cgggccgcag tcccgcaagc cgctgcttga gtctcgcacc aagcctgttc gcgcgcgcgc
     8701 acagctcttc ctcctggatg acatccagaa cagcatgggc cgccgctatt ccaagaggat
     8761 tgccgccata ggtgcccccc agccctcctg gattcggagc atccatgacc gacgcacggc
     8821 ccgtcaccgc cgcaagcggg aagccaccgg cgaggctttt tgccatcgtg accaggtccg
     8881 ggacaacgtc gtactgctcg atcgccagca tggttcccgt ccgtgcaaac ccggtctgca
     8941 cctcgtcaac tataagcagg atgccatact gatcgcatac ccccttcagc cgcttcatga
     9001 atgccggcgg cgcaacatag aatcctcctt ccccctgaac cggctcgata acaacggcgg
     9061 cgacgcggtt cggatcgaca tcggccttga acagcttgtc cagcgcggag agcgccatgt
     9121 cctcggtaac gccgtgcagt tcgaccggga aaggcacgtg atagacgtcc cctggcatgc
     9181 tgccgaagcc ggctttgtac ggcagcacct tgccggtcat cgccatgccc atgaatgtcc
     9241 ggccatggaa gccgccggag aaggcgacaa tggcattgcg ccccgtcgca gcccgcgcga
     9301 tcttgacggc gttttccagc gcctcggccc cagtggtcac gaagatcgtc ttcttctcgt
     9361 ccgggcccgg cacgatggcg ttaagccgct cggccagccg cacatagctc tcatagggca
     9421 cgacctgatg gcaggtatgg gtgaagcggt cgatctgttc atgtaccgct tgcatcaccc
     9481 tgggatgacg gtgaccggta ttgagcaccg cgattccggc cgcgaaatcg atatagcgat
     9541 tgccttcgac atcccagatc tcggcatttt cggcacgatc cacgaatagc tgggtcagca
     9601 cgcccacccc gcgcgcgatg gcacgcgatt tcctcagttg aatctcagca ttccccatga
     9661 cctgacttcc gctcctcgtt tcaacacggc gcaatggcga ggaggttagg aagatccgcg
     9721 cggccgagga atatgcgtaa agggatatcc ggccggctgt tgcgcaggcg cgcaattctc
     9781 agctaaggat atgttccgcc gggggaaatc cgacgtcagc ccccgccgcg gagcgcgcca
     9841 gcggcctatg gcggcgatcg gttccgaagt ccaaatgagc ttcagtcacg cgacattcgc
     9901 aaaatggaac ggcggcattg ccaagctcct ggaaagcgag agcaacggcg tctccttgct
     9961 ggaacaggca atccacgacg tcatcgattt cgacatattg atgatattca aatacaccgc
    10021 gaaccatgcg gaatgcctgc atcacaacat cgccgatgcc aggtcgaaaa tcgtcatcga
    10081 cgattatttg aagggcccct ttgttctcga tcctttttac agccatgcct tgatggaacg
    10141 aaaatccagc ttcggtgcga tgcgggacct gtctcccgat cacttccata agagcgagtt
    10201 ttaccgccag cattaccacc tgacgaacat agcggacgaa atcggcatat tcttcgggct
    10261 ggatccggca gtcacggccg tgttgagtat cacacgagaa aagagccaaa ggctgttttc
    10321 gagggcggaa aagacgctct tcgccgcgat ggcgccggtc attgaaacat tagggcgtaa
    10381 aatatggcag tccgatcggc gcgcgagacc tgatgccctt gaaagcagga tggagagcgc
    10441 atttgccgaa ttcggaaagc gggtgctgtc acgacgagag atggaagttg ccacactgat
    10501 actgaggggg cattcatcgc tgtccattgg caatatcctg gacatcagcg ccggtaccgt
    10561 caaaatccat cggaaaaaca tatacgcgaa gctgaacatc tcgtcgcagg cagaactctt
    10621 tcatcgcttc ctgcagctgt ttccatcata ggcggccggc gggttcttga gcattcttgc
    10681 ccggcaccgg ccttaagccc ggcgccggac agcaacccgc catactatcc gcggcaccgt
    10741 ccggggccgt caataaccgc gcttcagatc aacggtgttg catagccgct ccccgcggcg
    10801 cagcaaggct acgttatgga caatctgcgc cgccacggtg ttgggatcgg ccatggtggc
    10861 catatgcggg gtcacgatca cgcccggcaa ggtccagaac gggtggccgg atggcagcgg
    10921 ctcgtcctcg aaaacgtcga tgaccgcccc gcgcagatgg ccgctggcca gggcctgaac
    10981 caggtccggc tcgacgaggt gttcgccccg gccgcaatga accaaagccg caccgcgcgg
    11041 cagggcgtcg aagacgcgct tgcacaggat gccacgcgta tcatcggtca gcggcaacag
    11101 gcagaccagc acatcggtgc tggccagaaa cggcgccagg ccggcctcgc cgcggaatac
    11161 ggtcacccct tcccgcttgc gtgccgagcg cgaccagccg ttgaccgtgt agccgagcat
    11221 cgggagcacg gcggcaatgc ggttgcccat ctcgcccagc cccatgaggc cgacacggca
    11281 gtcggcggcc ggcagctgcg gcggccgatc ccacctgcag cttgactggt tcgccaggac
    11341 ggcatccatc ctgcgatggt aaagcaacac cgtccataac acgaattgca gcatgccctc
    11401 agcctgctgc gggtcgacca cccggcaaac ctgggcatgg tgaagatcct gccccgcgat
    11461 gatgttgtcc acgccagccg caatgctgtg caccagtctc agacgcggca tctcgtcgta
    11521 cataccgggc gggacttccc agccgacggc cacttcggct tcgcgaaaac gcgcatcggg
    11581 ccagacgacg acgtccagca agggatcggc ccgctccagg agcggcttga ggaaggacag
    11641 gtcatggcgg cggctgaaca gcgccagaac gatgcgcccg ttcatggacc cgggccggat
    11701 caatgacggt ccgagtctgc cgccatggcg gcgaaggccg gattcgaagg cggcgagacc
    11761 gggcctgaag cgacgttgcg ccgggatggt gctgcgtctg gggcggcggc agtctggcgg
    11821 tcgtaacgtc cggccttcat gacctgcccg ggcacatcat ggccgagcag ggcaggcagt
    11881 tgccgcgcca catcgatcaa tgcatcgaga tccacacctg tctctatgcc catcgcatcc
    11941 agcatatgca ccagatcctc gttcaccaca ttgccggtcg caccaggtgc gaacgggcag
    12001 ccgcccagtc cgccgagaga agcgtcgaaa tggcgcaccc cggccatcag ggcggcaagg
    12061 cagttcgcca ggcccatgcc gcgcgtgttg tggaaatggg cggtgaaccg aaaatcggga
    12121 aactcccgca aggcgacgag gaacagcgat tcgacctgag ccggctgggc catgccggtg
    12181 gtgtcgcata gcgtgatctg cccgataccg aaacggccga tcctttcgat cagacgcagg
    12241 acttcggcag cctgcaccgg cccttcatac gggcagccga aggcggtgga aagcgagaca
    12301 ttcacgatgg ccgcgcccat ggccagggaa tggatggcgg atatctggtc aagcgatgcc
    12361 gaccgctcca tcctgagatt cgacctgtta tgcgactcag agcaggacat gacgatgttc
    12421 acttcatcga cgccggcggc cagcgcccgc tcgcagccgc gtgcgttcgg aaccaatgcc
    12481 gcatagacga cgccaggcag ccgcgaaatt cccgccatca cggcttcacc gtccgccagc
    12541 gccggtatgg cgcgcggcga agtgaacgat gtcgcttcga ttttcgccac gcccgtgcgt
    12601 gagagcgcat cgatcagggc gatcttctgc ggcgtgggca ggaatgtcct ttccgcctgc
    12661 agcccatcgc gaggcgcgac ctcgtggatg gccaccgccg aaggcagagg ctgcaaacgg
    12721 ccggcggtca tgcaatgacc cccttctcct tcagatcgtt catcctctcc tcgccgacac
    12781 ccagcgcagc caggacgtca catgtgtgct cgccaaggcg cggcccgatc caggacgtgc
    12841 ggcccggcgt ggcgcataac cggggcacgg caccgggaag ggtcatgggc gtgccatctg
    12901 gcagctgcgc ggcctcgagc acgccgcggg cgcggaaatg cggatcggcg gctatatcgg
    12961 acgccgtgta aatagcgccg accgggacgc tggcggcgtt cagcgtctcg ataacggctg
    13021 gcgcgtccag cgtgagagcc caggcttgaa tcgccgcatc cagctcgccg gcatgcttga
    13081 cgcgtccttc gttgtcggcg agatcggctc gtgcggccag gtcggccctg ccgatgacct
    13141 gcatcagccg ctgaaagata ctgtcgccgt tgcccgctat ggcgacatat ttgccatcgc
    13201 ggcagacata tgtattggag ggtgcgatcc ccggcaggct ggcgccggaa cgctcccgca
    13261 catggcccaa taccgagtat tccggcatca ggctctccat gatggcgaac accgcctcgt
    13321 atagcgcgac gtcgacgacc tggccaacac ctcgcgatct cgcatgatgc agggcgatca
    13381 gcgttcctat ggtgccatag agcgacgcca gcgtgtcgcc caggctcacc ccggagcgca
    13441 ccgggggtcg atcggggaag cccgtgacat ggcgcaagcc gcccatgcat tcggcgatcg
    13501 ctgcaaaacc cggccggccc gcataagggc catcctggcc ataacccgaa atgcgcaaca
    13561 tgatcaggcg cggattgatg gcggagagca catcccagcc caagccccat ttctccagcg
    13621 tgcccggccg gaagttctcg atcacgatgt cggcgctggc ggcgatttcg cgcacaagcg
    13681 cgccgccctc cggtgtgcgc agatctatgg ccacagactt cttgttgcgg ctctgcgaat
    13741 accaccagag tgacgtgccg gcataggtct tgcgccactt gcgcagcgga tcgcccctgc
    13801 ccggaggctc caccttgatg acctcggcac cgaactgggc caacagggta cccgcgtagg
    13861 ggccggcgat cagggaaccg agttccagca cgcgcagccc cgcgagcgcg gcggcggggt
    13921 ccaccgtcat tcggcgctct cgccatcgaa ttcggctggc gagtaggtat agacgccagt
    13981 cggcccgcaa acttccgatg cgaatgcgag ctcacgtgca tcgggcatgc gcgcaggata
    14041 gagaatgccg tcgagatgat cgcattcatg ctgcaacaat gtcgcatgat agccgcggca
    14101 ttcacgctca tgccactcgc cgtcggccga ttgccagcgg atatggatat gcggatgacg
    14161 cggcacacgc gcgtacagcc ccggcaggga caggcagcgc tcccaaaggt cgaccgtctc
    14221 cgccgtcagc ggcgtgatct gcggattgat cagcggcgtc cacggcacgg gcctcgtgcg
    14281 ggctccggcc gggatgcggg aggcgggcag gcaatagagc acgacgcgca gcggcacccc
    14341 gatctgcggg gcggccagac cgatgccggc caccgaatcc aaggtttccc gcatgtcgtc
    14401 gatgagagcg ccgatctcgg gcgcacaagg gtccgctacc ggttcggaga cgaccgtaag
    14461 ggccgggtta cccatcttca gaatgctacg aatggccatg ggaggttcct tggtggggag
    14521 ggatcagtcg gtgcggcttg tcatggcgtc atataggcgg cgcaatggat cgggtccgac
    14581 gaagtggccc tgatccagaa ccggcacccc tccaacccac agcgactgat cgaatacgac
    14641 gccatccggg tgattctcgc cgccgccggc ctcccaccat ggcaatccca tgccgatggc
    14701 gttggttcca ggcagccgga tgtcctcgat gaactgctct ccggtcatgc gcgccgcagg
    14761 atgaaagccg taggtgaaat ggacaaaggt gccgagcgta ttcttgccgc tggcgcgccg
    14821 cagcgcacgc tcgaagctcg ccgcatcctc gggggctgcg ccctttatgg atttgattcc
    14881 tccatccgcc tcgatcagga taggcttgcg cagccgccgg tagctctcgt cgaacagggc
    14941 ctggatgaca atgcggccgc gcacggaatc cttcaccgga tacaggctct gcgcacccgg
    15001 caccgtatcc atgccgggcg ttcgcatgct gcgagaacgc ttgagcttca cctcgtccag
    15061 cacaaacgac acgtcggtcc ccgccgcgca ggtgaaccgt accgtctggc ccgccgcacc
    15121 ggcgacgaac tccatgaagg cgatattgaa gtccagcaat gcatcgaatt tgaccgcccc
    15181 gtacagcctc gacaggctgt ccgcacgcga gagcgcgatc aggccgtaac ggcagcgccg
    15241 tccctgcatg gcggcgtcat gaatgcgcga ccccgcatga tagggaaaac agaaatcaag
    15301 ccagacatcg gaagcgacca ccgcggcggc cagcggatcg cccacatagg gatcggacaa
    15361 accgccctgg aatggcaggg agggtgcaag cgcgaccaga ggcttggccc cctgtcgcac
    15421 caccgcctga acgacggtat cgataagggc gtgctcgacg gcggaatccg ccgtcagcag
    15481 cacgctctct ccgggggcaa cgcagaattg ctcgcgcacg agattgtcgg cggcacgggc
    15541 gagacgggga tgtgggaaca tgagctcttt cgcagaaatg atgtggaaag gaacgatgtc
    15601 aaaaatcgac gacgatgcga ggtccacgcg ctcgcgagac gcatagggcg atgtgcttgc
    15661 ctcgactgcg catatcctcg ctcaggaagc ggtcgcgatg atcgggttgt ccttcgatga
    15721 cctcgacgac gcacatcccg cattcaccct cccggcacga gaatggcaca tcgatgccag
    15781 cagccagcag gacagccagg gcgctttgac ccggcgggac cgtaaacggc ccctcgccgc
    15841 tcagcaggga tatctcgaac ggcgcatccg cgtcggcagc agcagccgca tcgccgtttc
    15901 cgccgaagct ttcccagtgg atgcgcgcgg caggcaggac atcagccgct tgcgcgcgca
    15961 ccagctcgat gagcggacga ggaccgcaga catagaggcc gtcatcgcgc gtgacctcag
    16021 tcagcagggc ggcgacggcg gcttgcacct cctctggccc caatccggcg tgcacccgca
    16081 ggtgcggcgc ataggctccc gccgagagcg catccaggta ggcggcctgc ctgggctcac
    16141 gcaggaagta atgcagcacg aaatcgtgtc cgcgtgcggc cgcggcctgc gccatggcat
    16201 ataatggcgt gataccgatg ccgccggcga cgagcacaag gcgatgctcg gtccagtctg
    16261 gcgcgaactg attgaagggg cccgccacag ccaatgcatc gccgatgcgc agggcgtgag
    16321 cggcggcgga tcctccgcgc gaatccgctt cccgtttgac agcaacgaca tagcggccgg
    16381 gcacgtcgcc gatattgcaa agcgagtagc agcgtaccag accggcgccg aagtcgagcc
    16441 gcagatgcgc ccccgcctcg accggccgcc agcaatcctc gggaacggca agggcaatct
    16501 cgacgatgtc gtcgctcagg cgccggatgt cttcgacctg cgcccgcgcc gctgcccctg
    16561 agacagcgac tggagcttgc tgaatatcgg cgtccatcga aacaccgtca gccgttggca
    16621 tcaccgaaca tgcgatcgac atagcttccc aggaaatggt ggatcggctt ctccatcacc
    16681 gacagccgcc ccggctcgta attgggaaga gccatcgcgc gctgcatgga ttcgatcatg
    16741 gaccgatcct cgtcgtagat cgccttctgg tactcgcggt atacgccgac cttctcctcg
    16801 aaattctcat gcgagaagaa ctcctccgga aacatcatgt agatcatgac ctcgcagctg
    16861 tccggtccgg tcggccaggc cgttatgatc ttgaccatgt cgattcgccc gaacagcgtc
    16921 atattgggcg gatagaaggc cgtgcaggcg aaggaattct cctcatgctc gagccagggc
    16981 gccttgccga acaggctgac gccgtcgggg gtcgagggcg aggccagata acgcatggta
    17041 agcccgccgc ccggcttgag aacgagattt tccggcgtcc acgagaagcg ctggccgaag
    17101 gttttggcgt ggagcacgcc cacatggtag taatccatca agttctcgtg gaagaacttc
    17161 caattgcact gcaaggtggt ggttgtaacg tcggccaggc gcacccgttc gctatgcagc
    17221 atcgcgaagt cgcgctccag atccgcgact gcttccgtaa acggtttagg ctgctcggca
    17281 aaattgatga agatgttgcc ccgccagata tccagatgga tgggcggcag gcgacacgcg
    17341 cctggctcga acccctcggt gtccgacata taggccgcgc cgacgagctg accgttaaga
    17401 tcgtaggtcc agccgtgata ggggcatttg aacgagcgcg cattgcccgt gccatcagcc
    17461 acctccacgc cgcggtgacg gcacatgttg tggaaggcct gtatcgcccc gtccctgttc
    17521 ttggcgatga gcacagggcg gcccagcacc ctgcgcgcct ggaaatcgcc cggcttctcg
    17581 aactgctcgg cgcgccccat gaagagccat tccttcgaga agtactcctc catctcacgt
    17641 gcgtagatct ccggcgaagt atagatgtcg ccggaaagat ggcgggaacg cgcaagctcg
    17701 gtcttgttga tggcgatgtc gccgcgcggc acgtagtggg acggtcgggt gggagttgtc
    17761 atggggcact ccggaggcat gaaggacggt acagacggcc gtcgatcggc gggcgtccgc
    17821 tggcagcata aactctatac gatcgtatta ttaaaagcaa gcatggactt cgccaggtgg
    17881 catcggcgca gatgcatgac ggcttgggat cgagtattat tgccgtccct tccttgagta
    17941 ccggatcgac atgaatgcag ttctgagcgt ctccgaccga aaaagctttt ccaagaaggc
    18001 ccccggtgtc cggcggcagg agttgatcga cgcaacgttc cgttgcttgc gcgactgcgg
    18061 tgtcgccgag accagcgttc gcacaattgc cgccgaggca ggtctttcgc tcggaatggt
    18121 acggcattat ttcaattcca aggacgaact cctcgccgcc acctaccgcg acatgtcgga
    18181 acggcttaag gagcagacgc aggagatcct ccgtcgcacc cccccggatc cgctgagcca
    18241 gcttgtcgcg ttcatcaatg ccggcctgca gcctccgctg ctggatcgca gctatgtgcg
    18301 catccgtttc ctgttcttcg aacttacgca caccaatccg gtggtcaaac gggtgcatga
    18361 agaaatctat tcccgcttcg agcggcagtt gctgacgctg gtcacggccg tcgccaagca
    18421 ttatggccgc gatgcggatt gccgcatgat cacccgcacg atcaccgctt atctgaaagg
    18481 catctgggct gaatggacgc tgtcgaacga cgacttcgag ccgcgtgaac tgctggcgca
    18541 attgatgccg ttctggcttc agcagctggc gcccgcggca gctctgaaag cggttctgcc
    18601 ggccaaggct cccaccaatg ccgtcaggcg ccgcacgcgc tcggcggcgg cgtcctgacc
    18661 tcaggcgagc aggcgccgcg ccgattgcca gaacagctgg ctgtgccagt cgctgctggc
    18721 gggatcgaat tgtgccgccg tgcggtcgag gacctcctgt gaaggcagat gcaacggact
    18781 gttgccggcc tgggccttga tctgcacctg gcaggcgcgc tcgaggaaat agatattggt
    18841 gaatgcggcg gcgatggacg tgcccgtcac aagcagtccg tggttgcgca agatcatggc
    18901 ttcgttgcgg ccgaggtcgc gtatcagacg ctcgcgttcc tcgagatcga cggcgatgcc
    18961 ctcatagtcg tggtagctca gccgcccgta gaacttgagg gcgtgctgcg agatcggcaa
    19021 cagcccggct ttctggcagg cgacggcgat gccgtccgcg gtatgggtat gcaccacaca
    19081 ggcgacgtcc gtccgcgcca tgtgaatggc ggagtgaatg acaaagccgg cacggttcac
    19141 ccgcttgtga ccctcgcgcc cgggatcgac gatgtcgccg tggatgtcga tcttgacgag
    19201 ttgatccggc ccgatctcat ggaacagccg gccatactcg ttgatcagga aatgctccgt
    19261 accgggcacg cgcgcgctga tatgcgtatc gatcacatcg gtcatacgga aatgggcgac
    19321 cagacgatag agggccgcaa gttcatcgcg ggtctgcgct tctgtgctca tgcaagggcc
    19381 tcgatatagg cgcgatcttg cgccagctgg cccaggaaat cggtaccggc cacggcgctc
    19441 accaattgac gcgtataggg atggcgcggc gcgcccagca cctcggcggg cgtgccggtc
    19501 tcgacgatcc ggccctgact cataaccacc acgcggtccg cgatttctcc ggccaccctg
    19561 aggtcatgcg tgatgaacag caaagtcagg ttgaactcac gcttgagatc gcccagcaga
    19621 tggaggatct gcgcctgaat cgatacatcc agcgccgata cggcttcatc ggcaatcaac
    19681 agcctgggct gcaaggccaa tgcccgcgct atcgagacgc gctgacgctg gccgccggag
    19741 aactcgtgcg gaaaccgctc tgcggcccag gccccgagcc caacgcggtc gagcagcaat
    19801 cgggcccggt tgcgggcggc cttcggcgcc tccccgtgga tgatgagccc gcgggcgata
    19861 gcctcgccta ccttctggcg tggattgagg gaggagaagg gatcctggaa gatcagctgt
    19921 gcatcgcgcc ggaagttggc caggtcgcgc cgcggcagct tgagaatatc gcgcccggca
    19981 aacaggatct cgccttggtc aggctcgacc agccgcatga tcatgcgtcc gagcgtcgac
    20041 ttccccgagc ccgattcgcc gacgatcgca accgtctccc cctcacggac gtccaggtcg
    20101 atgtcgcagg ccgccgcaac ctcgcgtgcc ggcgaccaga ggctgccgcc ggtcacgaag
    20161 cgcttggaca gccccctgat ctgcagcagc acctcgcggc tgccgcccgc ctcgtcctgt
    20221 gtcgcgggac ggcgacccgg caaggcgtct atcagcgccc gggtgtattc ggcacgcggc
    20281 ttcagcatga cctcatcgcg cgttccggcc tccaccgcgc aaccctgccg catgacgatg
    20341 acgcgatcgg cgatatcggc gacgacgccg atatcgtgcg ttatgaacag aacacccata
    20401 tggcgctcgc gctgcagcct gcgtatcaga tcgagaatct gcttctgcgt ggtgacgtcc
    20461 aacgcagtgg tcggctcatc ggccaagagc acgcgcggct ggttgatgag cgccatggcg
    20521 atcatcacgc gctgtcgctg gccgccggac agctgaaacg gatatgcccg gcaaatccgc
    20581 tccggctccg gcaaccccac cgccgccagg ctggccagca cacgctggcg cacctgctcc
    20641 gcccccaggg tccgggcgtg cagaatcagc gcctcggcga tctgcgcgcc gatacgctgc
    20701 aacgggttga gcgcgctcat cggttcctgg aacaccatgc cgatgcgatt gccgcgccat
    20761 tgctgcaggc cttgctcgcc gagttgcagg aggtcggtcc cgtcgcagac gatctcgctg
    20821 cccgcatcaa tacgcacctg gggcggcagc aggccgagga tggcatgggc ggtcatcgac
    20881 ttcccggagc cggactcgcc aaccaggcag acgatctcgt tcgggtggac atcgaaggac
    20941 agcccgtcga ccgcgaacag tcggtctccc gccgcgggca acgcgatgcg caggttgcgg
    21001 accttaagga gcggggcggc accggtcgtc attggcggct gatccgcgga ttgaggacgt
    21061 cgttgaggct ctcacccacc aggttgatgg ccagcaccgt caacagaatg gcaagtcccg
    21121 gaaagaagct catccaccaa gcctgacgga tcatcgtgcg ggcggtgccc accatatagc
    21181 cccagctcat catgttcgga tcgcccaatc ccataaagct gagcgcgctt tcgatgagga
    21241 tggcggtcgc aatgctgagc gagccggcga caaccaccgg cgacagcgcg ttcggaagga
    21301 tatagcggac catcagcgaa acgggcgatt cgcccaggca acgcgccgcc tggacatact
    21361 cgcgttcctt gagggaaagg aactcgccac gggtgaggcg cgcgacctga ggccagctga
    21421 cgatcgcgat ggcgaaggta atcgtcgcca ccgagggggt gagtatggcc accaggagaa
    21481 tggccagtat gaaagcgggg atcgactgga acaactcggt gacccccatg agcaggcttg
    21541 acgtgcgccc tccggaatac cccgctatgg cgccgaccag aactccgacg acgagggcca
    21601 tgaatgtgga ggtcacaccg ataaggaggg agattctcgc cccatgcacg acgccggcgg
    21661 ccacatcgcg gcccaggctg tcgctgccca tgagcagccc gtcggtgcct ggcggcgtga
    21721 atggcgtggc gactgtatcc cagggattgc cgggataaat cgccgatgcg aacagcgccg
    21781 caccgacgac gaagaccagt atgaccaggc cgattacgcc ggatggtctg cgcaggaagg
    21841 caagcaaggc cggtttcatg acactcacac gctgcgaatg cgcgggtcca ccacgcggta
    21901 cagaagatcg gtaaccagat tgaagatggc gacgacgatc gacatcacga gaaagatgcc
    21961 gagcagcagc tgatagtcgc gttgcagcac ggcttcatag gtcagccgcc ccagtccggg
    22021 ccatgcgaag atggtctcga tgacgacaga ccctccgatc aaggtgccgg cctgcacgcc
    22081 cgccatggtg acgacaggca gcagggcgtt gcgcaacata tgcctccaca gcacgcgact
    22141 gcggccggcg cccttggccc tggcggtctt cacaaaatcc tgatggctga cttcgatgat
    22201 cgacgcccgc atcaatcggg catagatggc cagatagagc aggccgagcg tcgccgccgg
    22261 caacagcaga tagcgccccc ggtcgagcca caacgcccaa ccggcaaggt cggcgccgac
    22321 ggtctgatag ccgaagggag gcagccattc gagctggacc gcgaacagga ggaccagcat
    22381 gatgccgagc cagaataccg gggtcgcata gaccacaagc gagagcaccg tgatgacggt
    22441 gtccgagaac ttccccgccc gcacgccggc caggccgccc agcacgatcc cgcccaccag
    22501 cgagatgatg aatgcggtgc cggtcagcag aatcgtcggc cccagccgct cggcgatgac
    22561 ttcggcaaca ggccgttgct gccggtagga atagccaaga tccagcgtga cgaccttgcg
    22621 cagatagccc gtaagctgtg tcgtcagagg ttgatcgaga ccatatgcgg cgcgcacccg
    22681 cgccatatag gcctcgtcgg tcgcccccgc ctgtccggcg atgaccagcg ccgggtcgcc
    22741 cggtgccgca tgcaccagaa agaagttgaa gatggcgact gcgataatca cacccaccat
    22801 cttcgcgaca cgagcaagca caggctttag cattgccacg gacagcaatg tcttatcgct
    22861 ccaattcgtc atggtccggg aagggcgaca tggcaggcga ccgtccttgc tccatcaccc
    22921 tttaaagtat acttcgtcga agctctcgtg gatgccgatg ccggtggtca ccacgttctg
    22981 caatcgcttg tcatagatgg tcggaaactc catctcgacc agccaggcga taggcgagtc
    23041 ttcaaccagg atcttctgca tctggctgta tagcgcctga cgcttgccct cgtccatctc
    23101 cgcagccgcg gcgtcggcaa gcctgtcgac ttcgggattg gagtaaccga tcgtgttgga
    23161 gaacagaacc ttgtggatgt tcgagctgac gtaaagccgg gtgacaccga tggcgggatc
    23221 gccatactgg aagggccagg tgagattgag gtcgtaatcc caattggcga ttttttgcgc
    23281 ccagccggcg gcgtcggagg tctccagcgt cacggctatg ccgatcttgg ccagggattg
    23341 gcggatgtac tcgcaggcgc gcgccggcat ttccccgtaa ggcgccaccg gcagcttgag
    23401 gcgcgcccgc accccttcgc tgtccggctt gagccccatc tcatcgagca gggcggtcgc
    23461 cttggcgatc gaataatcga atttcctgac attcccgtca tagaatcgcg tcttggagga
    23521 gatcgggccg gtcgcgacct tgccgaatcc gtacaggatc ttgtcccgca cgaattcccg
    23581 atcaatggcg tgcgaaatgg cataccggaa acgcttgtcg ttcagcggcg cactgcgcac
    23641 gttcatctcg agatacatga tgggggcaaa atattcatac cccttggtgg tcatgacgaa
    23701 tttgggattc ttcgccagcc gctgcgcatc gaacagttca agatccgtcc actgcgtgag
    23761 ctgaacctgc ccctcctcga tggccacggc gcgggaggcg ccgtccggaa cgatgcggta
    23821 gatgatcgca tccagatatg gcttgccggg tttgaaataa tcctcgttcc tcaccaaatg
    23881 gatatgcgag ccgcgcaccc actccttgaa cttgtacggg ccgctgccga tcggcttgaa
    23941 attggcggga ttcttctgga gatccgttcc ggcatagatg tgcttgggca tcaccggcag
    24001 ggtcgcgata tcgaacgccc ccagaaaggc ggcgaaaggc tgcttcagct tgaagacaac
    24061 ggtatggggg tccggggccg tcacggattc gcaccgagcg aagatcgcct tcgcccgggg
    24121 gttggttacc gggaggaact ccgtcaccgt gtaaaccacg tcgtcggccg tcagcggctg
    24181 gccgtcatgc catttgacat tctgctccag cttgaacgta taggtcttgc cgtcggccga
    24241 aatcgaccag cttttcgcca ggctcggcag gggcttcaga tcgaagtcgt aggtcagcag
    24301 gctcaggaat atctttcctg cgattgtctg cgtcggtgtc tgctggttga ccgcgatgtt
    24361 cagcataggc ggctccggtt gcagcaccgc gttgagcgtg cctccccgcg tctggccata
    24421 cgcgcgcgtc gagccgaagg aaagatcggc gaagcagatc gctccggcag aaagcaggag
    24481 cttattgaac tgtcgcttgg tcaccatcat ggaagacacc cggaagttgg attgagagtt
    24541 gggcaaaagg gatttacgaa caaggtgccg cgggggcggt caattcagca aattgcgttc
    24601 cagcagccac tgtcgtgtga acagggccgc ctcgcgcatg agctcgggct gcccggcata
    24661 gaaatgggag gcccctttga tcaggcggaa gctcttgtcg gcactccccc ccgcctcgaa
    24721 cagcatccgc gcgtggggct gcggcacgca gtcatccgcc tcgttctcga tcacaagcag
    24781 cggcactgaa atccgcgcgg cacatgccag cccatcgaca cggctgtcgt cgatcgacca
    24841 ttgcgacagc caggagcgca acgtcgagaa tcgcgcaagc ccgacaggac cggaattcac
    24901 ggtttctgga ttgccgagat aacaccagcc gggaggcctg ccattcggct cgatcgccgg
    24961 atccaggtag cgcggatcgg ccagggtgcg atgggtaacg aagccgcgct ccagttcacc
    25021 ggtgttgcgg cgctgcaacc tatgcagcgt ctcccatacc caggcggtga tgcgccggac
    25081 ccgtgccagc tggcgcttgc ggtactcggc aatgtagtcg ctgccgtaag gcggcttgtt
    25141 gggattggcc ggatcataaa gatcgaattc gacatctcga tcgtcgggat tgctttcgtc
    25201 gcgaacggaa gggtcgatca tgtcccgcag catgaccgcg cgcgacaggt gtgccgcctg
    25261 gcacaccacg gcgtcagccg caatcaagtt ggccccgacg agatccacgg gatcctgcgc
    25321 cggtgtggcg acgatgtcgg gccgctccgc ctgcgactgg tagaacagcg acagggaacc
    25381 gccgccgctc cagccggcga gtattatttt ttcatagcca agcacctcgc gcgcatgccg
    25441 gatatgtgcg ccgaggtcca tagcgacctt ctccatgatg agcgcgctgt cgttgcgcgg
    25501 gtaacggctg gcggcgcaca gcacatgcat accgaaggcg gcgagctcgc gcggaacagg
    25561 aagcagttgc aaggtcgagg cgggatgcat gaatacgaca agggttttcg atgccacgcc
    25621 ggccggcttg agcaggatgg cctcgagatt gaccgaaccc tgggtccccg cgaaaccgta
    25681 tgtctcagtg aacgcgaccg attccttgaa atgcacatga agccattgag cggaaacctc
    25741 aaagcggcga ggttctgtca tctgcattac tcccggggca acggaggacc gctcggcaag
    25801 cgtccgcgcg gacgccatcg cgacaatcac tctatacgac tgtatagctg gatgatgtca
    25861 gaaaagtgac gggttggcaa gcatttttgc gatagatcaa agatggtgca aaaaagcact
    25921 ttctcgcctt tcccgcttct ccgcagcctg ctcgcgcgcg agaaccgccg aacacgcgat
    25981 gcatccattt ccgttgcctg cgacagcccg cgtcgaagcg acggcgagat tgcccatcgt
    26041 caagccggct cccattgacg ccgcggcgcg cggggactca tcccgcctgc gggctgcctc
    26101 catgcattga ttggacagcc gccgggcgga tcaggccgac gatggcgggg cgcaacgtag
    26161 attggcaagg tgatggcggc caggctgcgg aattgcgcat gagcattgcc agcaatggcg
    26221 gcgcgcagat gcgcggcagc cagcggaaac acaatcaacg cacggcgtat tccgcccaag
    26281 cgagcctggg accggggcta ggccgggaac agggccttcc gcaggtcgat ctccgaccgt
    26341 tctttcgggg ccagccggac cccggcctcg agctcgtcca ggtgctcccg catcaggcgg
    26401 cgggcgctcc ttccgtctcc agaggcaatg gcatcgatga tgtggatgtg ctcatgccgg
    26461 ccgcaggtcg tttcgccgcc gcaccgtaca gggcgagtat caacgaggtc cgcgaaatca
    26521 gctccgaaag aaaatcgtac atcggagcat tcgcggcgat ctcggctatt gcgagatgaa
    26581 gctctcccga aaggcgcaag gccgccgctc tatttccgga gccgatcgcc ctgcgctcgt
    26641 cctgctccag cttacgcaac ttcgcgatcg aagctctcgt tgccttggcc gcagccagag
    26701 cagcggtatg gtcttcaacg actttgcggg cgtcgaagat ctgatgggct tcttgcacgg
    26761 agggctcgga gacgaatgcg ccccggttgg gcagaagagt gataatgcgg tcctttgaaa
    26821 gacgctgaaa tgcatgacgg atgatggtcc ggctgcaccc gaacaggccg gccaggctct
    26881 cttcactcaa tttcagaccg gggacgaggc gccgctcaat cacagcgccc tgaatggcgg
    26941 cgcatatggt tgcgatcttc tcgtttccta agctggcgac tgggtccatc cggtcacagg
    27001 ccggaagtcg ctgtttctgg gcggtgacgt cattgcaggc gtagagggaa tacctgagga
    27061 cgaagcactt gatctgatgc gcgaaatcct gcaattcgca acgcagccgc aatatgtcta
    27121 ccagcatagg tgggcggcag gagacatgct ttctgggaca atcgatgcac gctgcaccag
    27181 ggcagcaaat acgacaccgt caaccaccgc cgccggatgc accgcagcac cattgcggga
    27241 acagccgcac ccagcttctg acatgccgac acttctattc tacaatccga acagctcgac
    27301 ctcggtgacc gagaagatcc tcgaagtcgc caggcggaat gtgcccgacg ataccgaagt
    27361 tgtgggatac acctccccta aagggccgtc ttacatcgga tcagcgcccg atcttcttga
    27421 agcggctgtc gccgcgctgg attgggtcaa aaccgaggcg gccaagtatg atgccgtggt
    27481 gcttgcctgt ttcagtgata ccggcattgc ggcattgcgg caggccgctc ccgtgccgat
    27541 cgtcggactg atcgagacct ccgtcatggc cgcccaactt ctggggaagc ggttttccat
    27601 agtgacgggc ggcgacgaat ggcgccgatt gcttccgcca atgctgaacg cgatcggcca
    27661 gcagagcagg cttgcaagca tccgggcttt ggggcagtcc ggacttcaga tcatgaaaaa
    27721 ccccgaaaaa gcgtgccagg tgctggccga ggccacgcaa tccgctttcg atgacgacgg
    27781 tgccgacgtg gtgattctgg gcggagccgc gcttgccggt atggataccg ccgtgcaggc
    27841 ggcggtatcc gggatggtga tcgacagcgt cgcgcctgcc gcttggatgg cagccgcctt
    27901 ggcgcggaaa cgtccggttt ccatcctcca tccgcctgcc ggctgaagcc cgccatcggc
    27961 acgcgcccgg tgcagcggcc agtccggcgg catgctgcat tgcggcggca gccggcccct
    28021 gtggcgcaag atggtcaggc tgagccggaa agccctgccc ggcctatccg cctggccaaa
    28081 gccggttcgg gcgacctgaa cgccatcgac gagcggttca agggagcgta gcgactggtc
    28141 gatatccgaa atcttctcga caaggagtct ccgtcgaact gctccttcca tgaaggctgc
    28201 tgctaggacg atcgcagctc tatatttgca acgccgcgct gccaacggcg atgcagccga
    28261 ggaatgcgct cgatctccgc aaacagatcc accctttccg gccggcgaaa gtggtcatca
    28321 tcggcggcaa ccttgatcca gcgcctgcat ccgccaaccg ggacgccctg acgcaagccc
    28381 ctatgcgcgc cgccctttca gcaagcggcc gatccagatc aggatgcagg ccccgatgaa
    28441 gcctgcgaca agatagccga cccgcccgcc caggacgatg ccgaaccagc ttagaacggc
    28501 actggcaatg gcggccccca cgatgccgag gagaatattc aggaagacgc ccatatcgct
    28561 cttcatgaat ttctccgcga gccatccagc gaaacctccg atgatgatag cggcaatcca
    28621 gccaattccg gcagtctcca tgatgcattc tcccgaacag ctacccagca aatgggatga
    28681 tatgccgttt ggtccagtcc agaaattcat ctgaaaggcg gtccatcgca ggacgcccga
    28741 aacctgaacc gggttgtacg atcaagccga attcaaccgg gtcccgcacc atagacgtca
    28801 acaagcgtca accacgacgt gtagacattc ggctcgggat caacaagacg gatccacact
    28861 atctacaggc gtccgcacgc cgatggacgg cgcgccccag gctcaagcgg atcaccgtca
    28921 cggatcgccg gccagtggtg aaaacggcat catccagcgg cttggcaaac aggcacagcc
    28981 cactcatgga tggcagcccc ctccctcatg ttcgaggtcg ccacggggag cacctcacat
    29041 cgccctcggg taatggctgg aggcacaccg cgtgggtaag ctcaaatcgg cgggtgcgct
    29101 ctgacttaat gtcggccaat gtttgcggcg acgttctcca tacgcgcttc cagcgtcttg
    29161 cgcaggtcag cgggtagcag gtcgcgcgcg gcatgatcgc gccaagcggc gcgggtttcc
    29221 tcaacggtac ggaggattgt cttccatagg aatgggacgg aaagctgggc cgtatcggcg
    29281 aagcgccgca cctgatcggg cgtgatgtcg tccatcgcct tggatcgccc gaagccaagc
    29341 gcaagctgct gtttgggcag atagggcacg gtagatacat agtcgtaagc cggggccagc
    29401 accggcttac gcccgtccgg ataaagcagc gaccagtttt tcaggtgcat gtcggcgttg
    29461 ccgatcagga tcgagaacgc aagtcggcgc atgaagtcga tgacagccgc ctcgccaatc
    29521 tctgcggcca gaacggcggc gatattggcg tagctgcgct tgtcatactt gtcatcgggg
    29581 aagatgccga aaacctgggc gaagtcctcc atgtggacgc gcccgccgtc ggcggtgcgg
    29641 tcgaagcgct ccaccacgag cgccttgcca gcaacggcgc gggcctcatc gggcagcccg
    29701 gcaatcgcct ccaccggtat caggccgagc tttggcacct cgatgccaat acggcgtgcc
    29761 aactccatca tggcaaactc gttctcaggt acagccggaa agcgtgcgga gggcagcttg
    29821 acgatccact ccccgccgac gccaccggcg ggaatggtca ggccgccggc ggcctccatg
    29881 acggccgaaa acttgagctg caccccggca agggagaaac ggagcgcatt ggtgagagca
    29941 tcatgatgat gatcagcagc atgatcacga tcgccggcac catcctcatg gacagtgacg
    30001 gctccgggca gatcctcgcc aagggctgcc agcaggaaga actcacgctc cggctttacg
    30061 cctgcccgcg cggccaggta gtcgcgcaaa tggccttccg ggagcaggtt ggagaagaac
    30121 ggcggcagcc gcacgctgtg tggccggatg gctgtcacca ccccgcccgt ctggctcttg
    30181 aaggaaaggc tcaggattgg gcggttggga tcgtcgatgt aggcttcgtc gaaggcaaat
    30241 acatgcctgt cacccgccaa ccgggtgatg gtgccgacct ggcgatcgga caggcggacg
    30301 ataaggttgg agctgatagg cgccatcacg ccacttcctc gtcgttctcg ccacctgatg
    30361 catagagcgg acgttccgca tcctcagtgc cgtgagcggc acggttacga tgctcacggg
    30421 caaggctgct cacaattggc gtcagggcgc gcgggacgac aacgacatcg aggtccaagg
    30481 cgcgcaaaag gtcgagcaag gtgtcgaagc gcgggacgat cttcccagtc tcgatgccgg
    30541 agacatgctt ttggcccagg ccgaccttct cgcccagttc caactgactc cagtccttgg
    30601 caagccgcgc atcacgtatt tccgtccgca gcccctcggg caagctcttc attctcagcg
    30661 gcttcatacc atttacgccc tgcgctatag cgctcacggt atatatggta ttgcacatcg
    30721 tagctttcaa tatattttat taccttaaat accttctttc ataccatgaa tggtatttcc
    30781 gataacacgc ggagactgga gcagctattc tccgcaccac aggcgtaagc gctcagtgag
    30841 cccccttatc gggcggcgat ctcctgagtg ggggctgcca gttccatggc aggaggtcgg
    30901 caatgcgctt ggcgggatgg ctggcggtgc aggccagcac gttgcgcagc caagcttcgg
    30961 aattgacgtc gttgagcttg gcggtctcga tcagggaata gatggcggcg gcgcgctcgc
    31021 ccccgcgatc ggatccagca aacagccagt tcttgcgacc gacagggacg ccgcgcaagg
    31081 ccccgttcgg cggcattgtt atcggccatg ctgttcgccg ccgccgtacc tttgccattt
    31141 ccctgtcgtt gaccatggcg gcccgacctc tggtacttgg ctcggcggcg gaggtcaggc
    31201 aaggatgagc aacgggtatg cggcggaggg gatcggtatc actagcttgc aagcgaattc
    31261 gcctcgcgag cccaaccggc cagggtgcga tcgattggtt gacgaccggg accaaagcag
    31321 ttggggttgg caggaaagcg cgaatccacg ctgcttgact gcggcgcagt gcaagcattg
    31381 caggggagca cgatcatagc ttcagtgaag cggacctcga attctttgcg ccgaatgacg
    31441 gcttgcttcc aaagttgaga gaggccgatg aacgcggtga cgagggctgc ggacgctatc
    31501 cggcggcaga tgatctatcc ggcggaaacg ctgcttgcca tcaaggaacg gccggcgccg
    31561 aatggctgct cgagcagggc tggcggctgg atgaggcgga gcggaaatat ggcaaggatt
    31621 ggctataccg tgacatcccc ctctgaaagg aaactactcg gcctgctcaa taagcacctg
    31681 gcggacgagc gcaccgctta tgctgaccgg gacgtagccc ggcggccaag gaatatgcat
    31741 atcgccagga aaagcttctg caagcggtac gcgaggtcgg caagtccggc aatatcggac
    31801 tgatcctgaa gccgaacgcc ggatccagaa aaatgaagcg cgtttctatg gcaatagcgc
    31861 cggcatgcgg ggcagcctga aaactgcgct gcaggaactt gaccaagcgg aaaagatgct
    31921 gccggctatc caagaccgcg ccttgtacat ggccgtcgac gccagccatg ccaatccaaa
    31981 gagcaggaaa tccaaagagc aggatcggtg ggctgccgcg ggatgccgca cggcagttct
    32041 tcgcttcgca taatgcgcgt ctactcaatg ccgataagtc gcgccttacg gaaacggaga
    32101 agcggattgt cgaggcgcgc cgccacaata tccgcatcgc ctcgatgagc tatatgagcc
    32161 tgcaggagaa tgcgcttggc gtccatgtag gcaaaagcgc gagctcggga tggcgatgta
    32221 acgccggaat gtagcgtggc taccatgccg gcgctacgcg gcttcctcga ggcgacggac
    32281 ccgcggctgc ttgcgccgca aagccttagc tatgtcatac tgcgcctgca tcgccaacca
    32341 ggcgcaagcg gtactgacgc cggcgcgttc gaggcggatt gccagatccg ggctgatggc
    32401 ggctcggcca tgcaggacgc gcgacagggc aacccgcgac atgccgagcc gatcggccgc
    32461 ctcagtgacc gacagatcga gtggcgcaag cacttcctcg cgcaggatct cgccaggatg
    32521 aacgggctcc ctcataagcg cgaactgtat cgcatagcgt tacaatcatc aagtcattat
    32581 gccctacagc gacctgcggc tctgctagag ggggctgaat tctgccgcag tttcacgtga
    32641 tcaggcaacg acgggaaccg gcgctgcact cggcgacagt cactattccc tcgccgaaag
    32701 cgcggccgcg gcgggacaat gcggatgaca tcagggatga ggaatgattc aattttgtgc
    32761 ccacgtaaac agacgggacg atatgaaaat cggtttggtc acgtcggtta gtctcggcag
    32821 cggtcagcga agcgcagagc agcgtattcg cgatatggcc gcgcggtatc gcgtcaaacc
    32881 ggtgcgcacg aaactcgatg gctgggcaga cgatgtcgcc agattgtcgg atgcggatgt
    32941 cgcgcgagat gaggttattg atctgccaga tgaggttatt gatctggttg tggccctgca
    33001 gcgcgccggg ttcgtgacga cgaaggaggc catagacctg tgcggtgact ttctagccga
    33061 gcggcagtaa tgcccttcga tccgttcggc gatttcgtga atcgcggctg cctgcgatgc
    33121 tgagggtccg atccgggtct ctaattttcc gaaccttaaa ctccgttttt tccgacattc
    33181 agcgctttaa ctccgaaaat tccgggagcg gtcctcacat atggaaccgt caggacgggt
    33241 tcggcgccgc ggccttggtt atatgtttgt tttaaccaac ataatttgca tatatgttgg
    33301 ttaaaacaaa cacggtgctt gacaaggcat ctccaagggc tatgtgatga tttgagaaat
    33361 catattgagc attagagttg cttcaaacca acatgacgaa cacaagcaaa attacggagt
    33421 cgcctccggc tgcggtagaa gacgccctca ggcgtcttgg ccgcaatatc cggacggcgc
    33481 gggtgcgccg caagctgcgc atagaagatg tcgccaagcg gatcggcgcg tcccgcttca
    33541 ccgtgtccga tctcgaaaag ggcaagccgg ggacatcggc tgccgcgtat ttcggggcgc
    33601 tgtgggcgct cggcctgatc gaacaggccg ccgacctcgc ggaccccgac cgcgacgaag
    33661 agggcaaggc cctcgaaagc gcccgccatc cgacgcaagc gccgcgccgc aggaagctcg
    33721 acaatgactt ctgagcgcga gtgctacgtc tatattgtcc cgcccggcgc aaccgaattt
    33781 gtcaccgccg gtcgctttca ggtatccgaa acgcgagacg gcctgccgct cgggcagttc
    33841 gtctacggtc gccgctacct cgcgcggaac gatgccgtcg aactcgaccc ggtcgagctg
    33901 cgtcttgcgg gccgcaccta tgaaaccggg cggatgaaag gcgtcttcgg cgcgatccgc
    33961 gattccatgc cggacaattg ggggcggcgc gtcatcgaaa agcacagcgg gcatacgcag
    34021 ctcgaagaat tcgactacct gatggaaggc gcggatgatc gcgccggggc gctgggtttc
    34081 ggcctaggcg tcgagccgcc cgccccgcgc cggcaattta atcgcacgct cgatctcgat
    34141 cggcttcaaa aggctgccga cgccatcatc gccgacgatc cggaccgcgc cggatcggcg
    34201 cgcgcacaag cggaagagct gctgctcgaa ggcacatcga tgggcggcgc gcgccccaaa
    34261 accgtggtgc aggatgatca cgcgctttgg ctcgcgaagt tcggtcggca ggacgatcgc
    34321 tggaatcacc cgcgcgtcga gcacgcgctg ctgctgctcg cacgcgcgtg cgagatcgat
    34381 gccgccgaca gccgcatcga aacgatcggc ggacgcgatg tgctcctggt gcggcgcttc
    34441 gatcgggact ggtccggcga aggctaccac cggcaccgca tggtcagcgc tctcacgctg
    34501 ctgcaatccg aggacagcca ggccgaccgt ggccgctggt cctatcttct gctcgccgac
    34561 gaagtccggc gcgcgagcgc gcagcccgaa gcagatttgc gcgagctgtt cgcgcgcatg
    34621 tgcttcaacg cggcaatctc aaacctcgac gatcatccgc gcaatcacgc cctgctcgcc
    34681 aagggccgag catggcgcct gagccccgct tacgatctga cgccgaatcc ggtggtggcg
    34741 ctggaccggc gcgatctcgc aatggactgc gggccagccg gtcggtacgc gaacaaggcg
    34801 aacctgatcg gcgccgccgg gcggttcctg ctgggccagg atgaagccgc ggcgatcttc
    34861 gcgcgggtca cggaaaccgt gcgcggccag tggcacgcca cgatgcgccg cgccggtgtg
    34921 aacgaccgcg actgcgatgc gatcgccagc gcctttcttt atgacggact gttcttcgag
    34981 gcggcgggtc agaatgtttg aagaataagc ggattgaaga atggacattt atgactggtt
    35041 gaagcttctc ggagcggccg tcggcggcgg cgtaacggtc aaggtgctgg atatcctgta
    35101 tcaggaattc cgccgccggt ccgatagctc agcgtcagca aagcgattcg tcgatcaaca
    35161 tcttgatccg ttgctcaagg ctgccgacga agtcgccggc aagcttcgct cgctggctcc
    35221 tgaagctgct tcagagcatc cagggaatcg gccagcttct ccgacggggt aaccatgcac
    35281 tgcaccttca attagctttt ggcgctgcac tcccgttcgc ttctggcgct gcgtccggcc
    35341 gcgccagaag aggtaccgca ttcccctgcg actgcagctg agtcccaagt tcgctcttct
    35401 gcgagtaggc agtcaggggt ccggcaagag tgttcaccgc accagccagg ccgacaacaa
    35461 tcgtccagcc caaggtggcg gcaccttggc tcacatttat tcttcagatt gccatgcgag
    35521 atgcggttcc gcgcgagcct gccggccctg tccttcagct tgccggcatg aacgtcgcgg
    35581 atcatgcggt acgcctgcga tacgggatct tcggtatagc tcgcccggtc gggcttcttg
    35641 aactcgatga cgacgatgga cgccagcggc tggctttcct cgctaaaggc aagcgggtgg
    35701 ttgagatgag gatgtccggg cggttgccgg actcgctctc caccgcgggc aaagcgctga
    35761 gcggcaggtc tgaggacaaa aacgaatgga aggtgagccg ctcgtcgatg atccagagat
    35821 tctgctgggc gaacggtacg tcgt
//
LOCUS       DASVDU010000006        28298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_948776, whole genome shotgun sequence.
ACCESSION   DASVDU010000006 DASVDU010000000
VERSION     DASVDU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28298
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948776"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VF226_00370"
     CDS             complement(<1..808)
                     /locus_tag="VF226_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7072472.1"
                     /translation="MSSGTHVTVGDASIPVIGYGTWPLKDEECARGVATALQCGYRHI
                     DTAEMYGNEAAVGEGLRAGGVPRDEIWLTTKVWWENIGDGPLQRSAEASLKRLGVDQV
                     DLLLIHWPSKTIPLAESIRALNDAKRRGLTRHIGVSNFPTRMLDEAVALSEAPLIANQ
                     CEYHPHLDQSKVLAACRRHGMAFVSYCPLGRGAVGGVLEEPVVREIADRLGRTPAQVV
                     LRWHIQQPSVVAVPKSGNPKRIAENFDVFNFALDDADMQRLSALARPDGRV"
     gene            complement(1281..2153)
                     /locus_tag="VF226_00375"
     CDS             complement(1281..2153)
                     /locus_tag="VF226_00375"
                     /EC_number="3.11.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033978 - phosphonopyruvate hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate hydrolase"
                     /protein_id="HEX7072473.1"
                     /translation="MSAARDLKRKLAEKPLTHIIGAHNPLSARLAEQAGFDGIWASGF
                     ELSAAYGVPDASLISMTQHLDMARAMAEAVSIPVVADIDTGYGNAINVMHAVEKYEAA
                     GMAAVVIEDKMFPKDTSLLAGGRQELIRVEEFQGKIEAAIAARRDPDFLVIARVEALI
                     AGLGQDEAKMRGEAYAAAGADAILIHSKSKTPDEIVTFVENWSSPVPLVLVPTAYPQL
                     TEDDIVELGKVKLVIYGNHAIRAAVTGMERVLQQIRKDRGIQNVDKEIVPVTRIFDLQ
                     GVPGMKAREKRYLR"
     gene            2431..3003
                     /locus_tag="VF226_00380"
     CDS             2431..3003
                     /locus_tag="VF226_00380"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7072474.1"
                     /translation="MSHYRIIRQCVCAVAAASVALPMMAASASAQGFFSFLWSGGSRQ
                     TVSFPTSYAPKQIIVSFGDRKLYWIYRRGEAISYPIAVPRPESRWQGVTTVSAKKVNP
                     PWTPTPEMRRENPRLPAFMPGGHPRNPLGVRALYLGSSLYRIHGTDAPWTIGDAVSKG
                     CIRMYNEDVLDLYPRVPIGTKVTVTWKRFT"
     gene            3325..4329
                     /locus_tag="VF226_00385"
     CDS             3325..4329
                     /locus_tag="VF226_00385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7072475.1"
                     /translation="MTASIISVVVPVLNEVQGLQPLLDRLCPILDRIGPWEIVFVDDG
                     STDGTLDTIRAKHRSDPRIKAVSLSRNFGKEIAIAAGLRYACGDAVVTMDADLQHPPE
                     VIETFVEKWRAGYQVVYGERLDRSHEGRLRRFLSRAFYRTFNALSKCDIPEGAGDFRL
                     LDRRAVEAMNALGERSRFNKGLFSWIGFRSVGVPYVVADRVHGSSKWSFRSLLRFALD
                     GLTSFTTIPLRIWSLLGLAISLTAFASAIVFLVRTLLYGVDAPGFPSLIISVMIFAGV
                     QLISLGVIGEYLGRVYEEVKARPLFIVAEEIGAMGPSSRQDRAKPDGAAHHEAIGDRR
                     "
     gene            4316..5206
                     /locus_tag="VF226_00390"
     CDS             4316..5206
                     /locus_tag="VF226_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEX7072476.1"
                     /translation="MTVAEHSPAADHRPAILWADDFAMTGGVSAGIEQLIAAGRLSGT
                     SALVTTRHWPSHARRLLNLRSRIAIGFHLNLTLGGPLGPMPHLAPSGTFPNSARLLEL
                     GFAGRLDEAEIAEEITRQLDRFEDMIGQPPDVIDGHHHVHVYPQVRRALLQVLAKRYQ
                     GTKPMLRDTADNPLAITFRHASVGRSLGLSLLAIGFGARARALGFPTNLGFSGVSPFD
                     ERLPFSRELRRFFRRPGPDHLIMCHPGYADEELARIDTLVARREVELAALFLDSDLPA
                     RIRHPSRDAMGHISWGAGNA"
     gene            5199..5660
                     /locus_tag="VF226_00395"
     CDS             5199..5660
                     /locus_tag="VF226_00395"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX7072477.1"
                     /translation="MPESPWPTKRAGAVRPLVHYSGFLIAGLLALAADLLILEALILV
                     GLHPLIGRVFAISIAMVVSWLINRTITFAMPGPPSLREYLRFAAVSWTSQAVNYSIFG
                     AILILRPSVSPLLAVVLASMVSMFVSYFGFRYGVFTRGPAMARPAKPRTEK"
     gene            5657..6535
                     /locus_tag="VF226_00400"
     CDS             5657..6535
                     /locus_tag="VF226_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX7072478.1"
                     /translation="MSSLSADTVPIICGAAELFATYDVVFCDVWGVLHNGIEAYADAG
                     KALARFRAQGGTVILVSNSPASSSSLARLLDRIGVRRHAWDAAITSGDLTRSHITQRG
                     YRLIHHIGPERDQRVFAGLDVSPVALERAAAIVCTGVVDDRREAGESYRPLLSKALLR
                     RMPFVCGNPDLVVDVGGELLPCAGSVATIYEAMGGDVYWAGKPHQPAYEAARQLAEQI
                     RGRPVDKDRILAIGDALRTDLAGAADYGIDCLLVAHGIHRDEVMRAGRIDPVRLEKLL
                     RTSPHRPIAVIPALAH"
     gene            6625..7809
                     /locus_tag="VF226_00405"
     CDS             6625..7809
                     /locus_tag="VF226_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX7072479.1"
                     /translation="MPSTTLQEPSFTFGIEEEYHLVDRITRDLTPAPPELMDACLARL
                     GGQVSPEFLKSQIEIGTPACRTFREARRHLAALRGTIGEIATGFGCAPIAASTHPFAH
                     HLDQKTTERERYQNLAQEMQGVGRRLMVCGMHVHVAIEDYELRIDLMNQVRYFLPHLL
                     ALSASSPFWQGQDTGLKSYRLTVMQALPRTGMPGRFSGRAEYERTVDVLVKAGVIEDA
                     TKIWWDLRPSHRFPTLEERITDVCTRLDDALSVAALYVCICRMLYRQRRGNLTWRAYP
                     VFLLNENRWRAQRYGVQGTLFDLGKGTLAPFTDLLGEILTLIAEDAAALGCEAEVAHA
                     SEIAAEGTSADRQVATYERLRASGASDEEALKGVVDMLIHETLAGTRVPPPLQGPPAG
                     MS"
     gene            complement(7842..8471)
                     /locus_tag="VF226_00410"
     CDS             complement(7842..8471)
                     /locus_tag="VF226_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7072480.1"
                     /translation="MARAFHHTALRCTLYPVALLLVAMLCWSPAAVADTWVFDKKNSS
                     IRFSWDHLGLSRQSGRFLDFDGRLQFSPTDPEGGSVDVVIKTASLWTGVKEFDDNLKS
                     ESFFNADRYPLITFRSTWVRRTGEKTGEVDGELTIMGISKPVTLKVIWNFTGEHPFAA
                     VNPTYKGRWVSGFSASAKVQRSDWGLTRALPLISDDIWITIEAEFLLKD"
     gene            complement(8584..9339)
                     /locus_tag="VF226_00415"
     CDS             complement(8584..9339)
                     /locus_tag="VF226_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7072481.1"
                     /translation="MGRLDGRIALVTGAGAGIGLAIARCFAAEGAFVYLADVDGTAAE
                     RAAAGISEAGGSAAALTADVSRGQDVTAMFRAVEQRHAKLDVLVNNAGLNVRSDFRHL
                     TDADWVRIREVNLDGVVRVARDGFELLRKSGNASLINLSSIMASRGLRQLAGYSATKG
                     AVSALTRALAVEYAPFGIRVNALAPGFIETALTERVLRNPVINKALLDQTPLRRFGTG
                     DDVARAALFFASDDSAFVTGAELAVDGGMAAGL"
     gene            9630..10892
                     /gene="ccrA"
                     /locus_tag="VF226_00420"
     CDS             9630..10892
                     /gene="ccrA"
                     /locus_tag="VF226_00420"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX7072482.1"
                     /translation="MPATKDLYEIGEIPPLGHVPRRMYAWVIRKERHGEPDSAMQVEV
                     VPTWELDSHDVLVLVMAAGVNYNGVWAALGKPISTLDVHKHPYHIAGSDAAGIVWAVG
                     SKVKRWKVGDEVVIHCNQDDGDDEQCNGGDPMFSPSQRIWGYETPDGSFAQFARVQDR
                     QLMERPRHLTWEESACYTLTLATAYRMLFGHRPNILRPGHNVLVWGASGGLGSMAVQL
                     CATAGANAIGVVSDDNKRDFVMQLGAKGVINRKNFNCWGEMPKVGTPEYDAWFKEVRN
                     FGKAIWDITGKGNNVDFVFEHPGQATFPVSVFVVKRGGMVVICAGTTGYNLTMDARYL
                     WMHQKRVQGSHFANLLQASQANRLVIERRIDPCMSEVFAWEQIPKAHMRMMRNEHKPG
                     NMAVLVNAPATGLRTFEDVLEASRTRFG"
     gene            11021..11479
                     /locus_tag="VF226_00425"
     CDS             11021..11479
                     /locus_tag="VF226_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072483.1"
                     /translation="MAKDPASPFRTRVRVKGVATEAVPSDPAAHETAPADSRLSLMEA
                     LKRAKPPPPSGKDQQPEVERSESQHAPRPAPVVARWKQEQTDAEPPRRVRRPSRRVGR
                     LAAAKLRQLARLKEAASSLESLARGEFEEATVEIVRRDRKTLATDSRTRD"
     gene            11711..13375
                     /locus_tag="VF226_00430"
     CDS             11711..13375
                     /locus_tag="VF226_00430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7072484.1"
                     /translation="MTNAAPPATYPSTTVLAEAGSVISAAERVLQAAKASMQGKLAAS
                     GSLDDCQHAAHSLSWLATYVEAMRQLGGWANRLAEERALGELEQLLLTIGLAEYAAQI
                     AGGIPMSQGEFARLKDIGVETVEIRRFEDAIADFVAEGTGDGVKRRLAELIAELPPGE
                     VIGNAGLDETHTAIQEQMHRFCEAEVTPHAQSWHLTNSYIPLETIEKLSELGVFGITL
                     PEDYGGLGLGKEAMCVVSEELSRGYIGVGSLGTRSEIAGELILGSGTEEQKQKYLPKI
                     ASGEILPTAVFTEPDTGSDLASLKTRAVREGDVYKVTGQKTWITHPVRADLMTLLVRT
                     DPNEPGYKGLSMLLAEKPRGTDDDPFPAPGMSGGEIEVLGYRGMKEFDISFDGFEVPV
                     SQILGGVEGQGFKQLMATFESARIQTAARAVGVAQSALDLALAYARERVQFGRPIYDF
                     PRVANKIVMMAVEVMIARQLTYFAAREKDLGRRCDLEAGMAKLLAARVAWAAADNALQ
                     IHGGNGFALEYPISRVLCDARILNIFEGAAEIQAQVIARRLLEGAN"
     gene            13496..14692
                     /locus_tag="VF226_00435"
     CDS             13496..14692
                     /locus_tag="VF226_00435"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7072485.1"
                     /translation="MATPSSDPVVIVSAARTPVGGFQGAFASVAAPELGAVAIKAAVE
                     KSGVEPEEVSEVLMGCVLPAGIGQAPARQASRGAGLPDHVPCTTVNKMCGSGMKTVMM
                     AYESLHARPNDIILAGGMESMTNAPYLLPKMRSGQRLGHGQVYDHMFLDGLEDAYDKG
                     RLMGTYAEDTAQAYQFTREAQDAFALESLKRARRANEDGSFAKEIVPVTVKSRKGAVE
                     VTRDEQPFTADPAKIPQLKPAFREGGTVTPANSSSISDGAAALLLMRESEANRRKLNP
                     LARIVGTATHAQAPAWFTTAPIGAISKLLEMVGWRAQDVGLFEVNEAFAVVTMAAMRD
                     LNLPHDKVNVHGGACALGHPIGASGARIIVTLLSAMEKYGETKGVASLCIGGGEATAI
                     ALERVV"
     gene            14720..15532
                     /locus_tag="VF226_00440"
     CDS             14720..15532
                     /locus_tag="VF226_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7072486.1"
                     /translation="MHVLSRVLFFLVQPSHFALILVGAGLLLLFIRPAAARTGKMLAA
                     TGFLSLVVLGFSPIGHALLLPLEERFPQGPMPAGKVAGIIMLGGFEDPGVSQARGALA
                     LNESAERLMAPILLARKFPEARIVFTGGASDIFLRSDSAAPFVATFLTAAGVAPDRIV
                     LEGKSRNTYENAIYTRSLVDSGPGERWLLVTSAWHMPRAVGAFRRAGFDVVPWPTDYR
                     TRGSADLLQPFDKMTEGLRRTDMAVREWIGLAAYWLLGRSDSLFPGPAPNPA"
     gene            complement(15545..16138)
                     /locus_tag="VF226_00445"
     CDS             complement(15545..16138)
                     /locus_tag="VF226_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072487.1"
                     /translation="MILVDLLLISGRSLRFKRGLPQICPVRVISSAGKSGHTYFMFKP
                     EVQIVLLAAGLVCGCAGAQSNAIDQSSASEQRPAAESPAQAGLQPDGSYVLSASELAL
                     NCHKLAGRTLVRILQIRDYELTRKGSLAARGLQKVVTPVFGGTERGADPDADYRRDRA
                     MVEAYNRRLAEKGCKSFDLVKELQPKPAAESPKLEKR"
     gene            16155..16562
                     /locus_tag="VF226_00450"
     CDS             16155..16562
                     /locus_tag="VF226_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7072488.1"
                     /translation="MPQKIMMGAKALVAAAKAEITEYSAGEAIELSKRPDVLLVDIRD
                     PRELAREGKIPGAFHCPRGMLEFWIDPESPYFKPVFGEDRTFIFFCAGGMRSALATKT
                     AQDMGLKPVAHIIGGFGAWRSAGGPIEGPATDR"
     gene            complement(16596..16775)
                     /locus_tag="VF226_00455"
     CDS             complement(16596..16775)
                     /locus_tag="VF226_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072489.1"
                     /translation="MLNQPFHDTEEIEEAGAAIMTGMRLLAEHLDPPTAHALATQLLD
                     ELLRDLLKDGSHALH"
     gene            complement(16983..17831)
                     /locus_tag="VF226_00460"
     CDS             complement(16983..17831)
                     /locus_tag="VF226_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072490.1"
                     /translation="MSADTPPKAQQPQPGHEHKMHPAPEYVPRFKGSGRLEDRIALIT
                     GGDSGIGRAVAIAMAREGADIAFVYLEEQRDADDTVQLIEAENKQALAIKGDIGDEAF
                     CQDVVRRTVDTFGKLDILVNNAAEQHLRNRLEDISAEQLERTFRSNVFGYFYMTKAAL
                     PHLREGSSIINTTSITAYKGSAHLMDYSATRGAIVSFTRSLSQAVVDRGIRVNGVAPG
                     PIWTPLVASSYPAEGIESFGKNTPMGRAGQPREVAPCYVFLASEDSSYMTGQVLHPNG
                     GTVVGG"
     gene            complement(17935..18366)
                     /locus_tag="VF226_00465"
     CDS             complement(17935..18366)
                     /locus_tag="VF226_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072491.1"
                     /translation="MPGSVATMEERDAITETAVSPELLAKAVQAVLRLEPAQAVTLRG
                     RAEAILDMLIDEGVVEGIVETGIAIQARLDAYVELMSCPAMAEFHPARGLVSEECVLR
                     AISQAPLRLREGRYRFCMRQLVILLSEDAAETGGEKPDLVN"
     gene            complement(18409..20862)
                     /locus_tag="VF226_00470"
     CDS             complement(18409..20862)
                     /locus_tag="VF226_00470"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7072492.1"
                     /translation="MAYIPVLESWPFAGTRRPRPRPRTIGEYLAVYALAVAAPILVLS
                     GIALHKNVAAERERLEQQSLQIVRLLKSDVDRELSSVILALQTLAASPSLDRGDLAAF
                     HNFASKLVREGSDLYIVLSDLSGQPLVDTRVQWGGALPASESASTEFRNVVRTKEPYV
                     SGLASGEASFTITVPVLRKNEPSALITAAFDSSRLSKLFQSAPVSDGWEYGIIDGQGR
                     LIAHSAGFELDSAKMPPRIRADQADRVELVSAFGRDEFQAVARSTIASWLVMASAPLD
                     VLLPGLWSWQYLALGGGALLSLSLLAALYFGSRLASPIRMTALAASAFGKGEAMPELA
                     LRLREVDEVLSALRAASVQRRLAEDQLRIAHERMSLALSATDMGMWERDLATNHVTWS
                     EAMYRLFGRSVDEFSGDPDQVVSYVHPDDRVRFRQAHQDAIRGPADTFDQEFRIVLPS
                     GEERWLYRRAFVRRNSDGVAISVLGVVIDITERKKAENANAYLAAIVASSSEAILSAS
                     PDGIIATWNTGAANTFGYSSAEAVGRPLQDLFLDEHSSGMERISAAMAKGETVRLESV
                     CRTKYGHTMEVSIIANPVYGHSGDIAGYSITIRDISVRKEHDRHLASVLRELTHRSKN
                     LLAIIQAMARQTALRSEGLADFEQRFSGRLQSLSRSHELLISNDWEGALLGDLVAVQR
                     SAFGSRHSRIRASGPDVFLRPEAILNIGLALHELASNAERHGALSVATGRVDLSWSVE
                     RGKGDEPRLLIVWRERGRQSAGDLLHRGFGRDILEHVTPTALGANVVLKAIPDGLLWT
                     LDVPGDQFAKLRLAAVASA"
     gene            21577..23556
                     /locus_tag="VF226_00475"
     CDS             21577..23556
                     /locus_tag="VF226_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7072493.1"
                     /translation="MELSRYKRARAAAAPSLPGSLLTAALVIAGLYFGSDLFVPLAIA
                     VLLSFVLTPLVVGLRRFRIPRAIAVVLVVVSAFSIIFGLGALMGGQVTALLERLPQYE
                     WTLRQKIRDLRPQPGAPAGIVERTTETLQNLGKELDERSESPSLAQPKTSAGPQPIPV
                     EIHSPPPAALEYFQRIVQPLLEPLASTGLVLILVIFILLQREDLRDRLIRLFGVADLG
                     RTTDAIDDAARRLSRLYLTLTTMNAVYGAGIAVALWLIGLPNPLLWGILAGLLRFVPY
                     IGTVIALVFPVLLAAAVEPGWSLAALTLIIFIVGEFTMGQVLEPWLLGNSTGLSPLAV
                     IAAASFWTWLWGPIGLLLAIPLTVSLMVLGRHVPQLNFIYVLLGDEPALTAAQRFYQR
                     MLAGDIDEITYDAEQFVKSKSLLCYFDEVALPALVMAQADVRRERLGPDRLSEMRELI
                     GELIEELEDVEIGKPPPKAGKEDEEQPSDLPVLAPEELKPGWRVAEPVLSVGVRNPLD
                     QAAAEILAHLAAGYGVGTRVIGASEVTTAAINQLDIADAKLAVLSNVDTARAPAHARL
                     MMRRLRRRNPNLKFLIGAWGESEAGSGPEAVAPMESGPAIQRMTSLRSALGEILREAR
                     AEPSSVPIPARKVEQPEPCSVGELQPEPAAQRRPA"
     gene            complement(23538..24920)
                     /gene="der"
                     /locus_tag="VF226_00480"
     CDS             complement(23538..24920)
                     /gene="der"
                     /locus_tag="VF226_00480"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7072494.1"
                     /translation="MTSRPNIVIIGRPNVGKSTLFNRLIGRRAALVSDMPGLTRDRRE
                     GTARLGAYEVNLVDTAGLEEAAPGTIAARMTRQTEQALKSADLALFVIDAREGITPAD
                     AAFARVVRAAGRPCILVANKCEGRKGLDGFYESFELGLGEPVAISAEHGEGIGELVSE
                     MLRALGLEDWAPAKKGSRAEEQIEAEDADARPIRVAIVGRPNSGKSTLVNALLGEERL
                     LTGPEPGLTRDAIATDFEWRGRKLSLIDTAGLRRKARITELAEKLATSDTMRAIRYAE
                     VVILMIDAERPLEHQDLTIGDLVAEEGRALVIAVNKWDLIENKQAVLRDLRESVAEKL
                     AQVPGVPVVPISALAERGLDRLLAAVLQVYEAWNRRVPTPDLNRWLREAVEQHAPPAS
                     KGRRIKIRYIAQVSTRPPTFVAFASQPKGLPKAYVRYLVNGIRETFDLPGVPIRFMLR
                     KGENPYAGRR"
     gene            complement(25003..26358)
                     /locus_tag="VF226_00485"
     CDS             complement(25003..26358)
                     /locus_tag="VF226_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7072495.1"
                     /translation="MGIAGKRSQRLGQILAPTLLVAIGLGGCGGSSLPSLPKITDLNP
                     FAEKEHPLPGKRIPVMQARDTIGGELATADKPVVLPAPRANEEWTQPGGVPSNAPGHL
                     ALGGSVKRAWSADVGSGSSTKGKLTASPLVYGGRVFALDATAKVTAVSASGGSVVWRT
                     SLVPEGEREESGYGGGLAIDNGRLYVATGFGTVVALDPATGKTLWTKNIGVPIRSSPT
                     AVDDKVFVVTTEGHVFALAGFDGGELWTFRGLPERTSIISNPSPAVDAGVVIVPYPSG
                     ELVALHEQDGTPVWSESLARTRTVSSLASLSDAARPVIADGVVYGVGHAGRLIATRIK
                     TGERLWSLNVPGTQPPVIAGNNIFVVDTNGQLHALTRQDGKVIWSAKLPGTSTWSGPT
                     LAGNQLWLTSNKGQLVGVDPATGRVTVQQGVGGPVYIAPIVAGGRMYVLTDEARLVAY
                     N"
     gene            complement(26371..27102)
                     /locus_tag="VF226_00490"
     CDS             complement(26371..27102)
                     /locus_tag="VF226_00490"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7072496.1"
                     /translation="MVDERDAMLRELESEVRREQMAKLWDRYGIYAIVVAVLIVASVG
                     GYKWWEARTLSAAAEAGARYQTAMQLVEQGKADEARQAFQDLVQNAPSGYATLARLQL
                     AGEAASAGQTDEALRSFEAIAADGSVDPLLRDYARLQTAALIIDRADFTEMQNRLTPL
                     MQDSNAWRHSARELLGLSAYRAGRLEEARQIFLELAADQKTPPSVRERSGIMMGLITS
                     AEAQQTTGSTQPVSGGKPAESPKVQ"
     gene            complement(27302..27886)
                     /locus_tag="VF226_00495"
     CDS             complement(27302..27886)
                     /locus_tag="VF226_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX7072497.1"
                     /translation="MLPLVLGLLVFFLVHLVPTEADLRRGLAGRLGENRYKAIFSVVA
                     LVGLVLIIIGYGKLQVMAGKNPEIWTPPIWTRHIAFLLMLPAMVLIVAAYVPSHIRTA
                     TKHPMLAGIKLWATAHLLANGDLASILLFGSFLAYAVYDRISVKRRSVKIPAAPHGWV
                     NDIIAIAAGLALYGFMLTVGHAWLIGVPLLPGQS"
BASE COUNT         5049 a         9234 c         8838 g         5177 t
ORIGIN      
        1 cgacccggcc gtccggacgc gccagtgctg acagccgttg catgtcagcg tcgtcgagcg
       61 cgaaattgaa tacgtcgaag ttctcggcga tgcgcttggg attacccgac ttcggcacgg
      121 cgaccacgct cggctgctgg atgtgccagc gcaggacgac ctgtgccggc gtcctcccga
      181 ggcggtctgc gatctcacgg acgacaggct cctcgagcac gccgccgacc gctccgcgtc
      241 ccagagggca ataggagacg aacgccattc cgtgccgccg gcaagcggcg aggaccttcg
      301 actggtcgag gtggggatgg tattcgcact gattggcgat gagcggcgct tccgacagag
      361 ccacagcctc gtccagcatg cgagtaggga agttcgatac cccgatgtgc cgcgtcagcc
      421 cccgccgctt cgcatcattg agcgcgcgga tcgactcagc gagcgggatc gtcttgctcg
      481 gccagtggat cagcagcaga tcgacctgat cgaccccgag ccgcttgagg ctggcctccg
      541 ccgagcgctg cagggggcca tcgccgatgt tctcccacca caccttggtc gtcagccaga
      601 tctcatcgcg cggaacgccg cctgccctca gcccttcccc gacggccgcc tcgttgccgt
      661 acatctcggc cgtatcgatg tgccggtacc cgcactggag tgccgtcgcg acgccccggg
      721 cacactcctc atccttcagt ggccaagtac cgtagccgat gaccggtatg gaagcgtcgc
      781 cgacggtgac atgcgtaccg gagctcatgg agggcatcct ttctggcgct gcaggggtgt
      841 cccctgacat gtccgccagt cagcacgccg cgtcaagccc gggtctggct gaagccgaca
      901 ccccttcggc gaaaaacacc ccttcggcga aaaaaagggc acccgcaggg ggtgccctta
      961 gatatgaccc gcgcgtgggg ggggggggac ggcgcaggtc atggacagaa cgcttgtgat
     1021 tagaatcagt tccaaccaga tctcaaaaag aatcgcaacc cctctgaaac tgctaaagga
     1081 agtcttacca gaggccggaa ctgcccggcg agcccgctgg ggaacgccgt tagcaccaga
     1141 accgccgctt tgcctatgct ggcgccgctt ccaggggcca aacgcctgac gcgggccccc
     1201 gggtcgcgca actctcgccg ggagaacaac atcagggcgc gctctgttcc ccacaccggt
     1261 tgagcctgcg acagcctgcc ctatcgcagg tagcgcttct cccgcgcctt catgccaggc
     1321 acgccttgga gatcgaaaat gcgcgtcacg ggcacgatct ccttgtcgac attctgtatg
     1381 ccgcgatcct tacggatctg ttgcagcacc ctctccatgc cggtcaccgc cgcccggatg
     1441 gcgtgattgc cgtagatgac gagcttcacc ttgccgagct cgacgatgtc gtcctcggtg
     1501 agttgcggat aggccgtcgg aacgaggacg agcggcaccg gactggacca gttctcgacg
     1561 aaggtgacga tctcgtccgg tgtcttcgac ttcgaatgga tcaggatggc gtcggcgcct
     1621 gcggccgcgt aggcctctcc gcgcatcttg gcctcgtcct gtccgagacc ggcgatcaag
     1681 gcctcgacgc gggcaatgac gagaaagtcc ggatcgcggc gggctgcgat ggccgcctcg
     1741 atcttgcctt ggaactcctc gacccggatc agctcctggc ggccgccggc aagcaggctg
     1801 gtgtccttgg ggaacatctt gtcctcgatg accacggcag ccattcccgc cgcctcatat
     1861 ttttccacgg cgtgcatcac gttgatggca ttgccgtagc cggtatcgat atcggcgacg
     1921 accggaatgc tcaccgcctc ggccatggcg cgcgccatgt cgagatgctg cgtcatgctg
     1981 atgaggctcg cgtccggtac accgtaggca gcggatagct cgaagccact cgcccaaatg
     2041 ccgtcgaaac ccgcctgctc cgccagccgc gcgctcagtg gattatgagc gccgatgata
     2101 tgtgtcagcg gtttttcggc gagcttcctc ttgagatctc tggctgcgga catccgttgc
     2161 cctctgtttc tgctcaaatc cagaaaaacg gcctgagcac gatccggcga acaggttcaa
     2221 tttgtcaatt cagaatgcaa accgtgttgg ttttcagtca ctgccctgcc gcaatttcga
     2281 cggtgccttg acaaattgac ggttgactga cgacatggcg caataccccg ttaacggcac
     2341 accagcataa tgcggatgct tgtgccgcgc atcttcggat cccgatcggc gacaacgacc
     2401 tgaagagttc caatgatggg atggaggaaa atgtcgcatt accgtatcat ccgccaatgc
     2461 gtttgtgctg tcgccgccgc gagcgtcgcg ctgcccatga tggcggcgag cgcctcagct
     2521 cagggcttct tctcgttcct gtggagcggc ggttcacgcc agacggtctc gttccccacg
     2581 agctatgctc ccaaacagat catcgtcagc ttcggtgatc gtaagcttta ctggatctat
     2641 cgtcgtggcg aagccatcag ctacccgatt gccgttccgc ggccggagag ccgttggcaa
     2701 ggggtgacca ccgtctcggc caagaaggtc aacccgcctt ggacaccgac gcctgagatg
     2761 cgccgcgaga acccccgtct gccggccttc atgccgggtg ggcatccgcg caatccgctg
     2821 ggcgtgcgcg cactctacct cggctcgtcg ctctatcgca tccacggcac cgatgcgccc
     2881 tggaccatcg gcgacgcggt ctcgaaaggc tgcatccgca tgtacaatga ggatgtgctt
     2941 gatctttatc cgcgtgtgcc catcggcacc aaggtcaccg tgacctggaa gcggttcact
     3001 tgaaaatccc tcatcgcctt ttctccactc gatgcggccc ctctgggccg cattttgcgc
     3061 tgccggcggc tcgagaatac ttctgcggct caccctgagg cgtgaggcca cccctgtcag
     3121 ggggaaatcg gaaccgcgct gtggcattcc gccaccacga cctgccaccc gcatctctga
     3181 aattatttgc catcgcttac acagcggtca aaaatcgggc ttggagttgc tctttgttcg
     3241 ccgggctcga ctcgtttcgg aaccgaagcc cgaaccgcag agtgcgccag gatgcacgcg
     3301 ccgtctgctg gaacgtgaat gcccatgacc gcctcgatca tctccgtcgt cgttcctgtg
     3361 ctgaacgagg tccaggggct acagcccctg ctcgatcggc tttgccccat tctcgaccgg
     3421 atcggaccat gggaaatcgt tttcgttgac gatggctcta ccgacggcac actcgacacg
     3481 atccgcgcca agcatcggtc ggatccgcgc atcaaggccg tctcgctgag ccgcaacttc
     3541 ggcaaggaga tcgccatcgc ggccgggctg cgctacgcgt gcggcgacgc ggtcgtgacc
     3601 atggatgccg acttgcagca tccgcccgag gtcattgaga ccttcgtaga gaaatggcgg
     3661 gcaggttatc aagtggtcta cggagagcgg cttgatcgtt cccatgaagg ccggttgcgg
     3721 cggtttctct cgagggcctt ctaccggacc ttcaacgcgc tctccaaatg cgacattccc
     3781 gagggcgccg gcgattttcg gctgctcgac cggcgcgccg tggaggccat gaacgccctc
     3841 ggcgagcgat cgcgattcaa caaggggctc ttttcctgga tcggcttccg atccgtcggc
     3901 gttccttatg tcgtcgcaga cagagtccat ggctcatcga aatggagttt caggagcttg
     3961 ctcaggttcg cgctcgacgg cctgacctcg ttcacgacca tcccgctgcg catctggtcg
     4021 ctgctgggcc ttgcgatctc gctgaccgcg tttgcgagcg caatcgtgtt cctggttcga
     4081 accctgctct acggggtcga tgcgccgggc ttcccatcgt tgatcatctc ggtcatgatt
     4141 ttcgccggcg tccagctgat ttcactcggc gtgatcggcg agtacctggg ccgggtctac
     4201 gaggaggtca aggcgcgccc tctgttcatc gtggcagagg aaatcggcgc catgggcccg
     4261 tccagccgac aggatcgcgc aaaaccagat ggtgcagcac atcacgaggc aatcggtgac
     4321 cgtcgctgag cattccccgg ctgccgacca tcgaccggct attctctggg ccgatgattt
     4381 tgccatgacc ggcggcgtga gcgccggcat cgaacagcta atcgcggcgg gccggctttc
     4441 gggaacgagc gcgctggtga cgacgcggca ctggccctcg cacgcccgcc gccttttgaa
     4501 ccttcgatcc cggatcgcca tcggcttcca tctcaacctg acgcttggag ggccgctcgg
     4561 gccgatgccg cacctggcgc cttccggcac ttttccgaac tccgcgcggc tgctcgagct
     4621 cggcttcgcc ggccggctcg acgaagccga aatcgcagag gagatcactc ggcaactcga
     4681 ccgcttcgag gatatgatcg gccaacctcc cgacgtgatt gacgggcacc atcatgtcca
     4741 cgtctatccg caggtgcgcc gggcgctgct ccaagtcctg gcgaaacgct accaggggac
     4801 aaagcccatg ttgcgggata ccgcggacaa tcccctcgcg atcaccttcc gtcacgcaag
     4861 cgtaggcaga tcgctcggtc tttctctgct cgcaatcggg ttcggcgctc gcgcccgggc
     4921 ccttggcttc cccaccaacc tcggtttctc cggagtgtcg cccttcgacg aacggctgcc
     4981 cttctcgcga gagctaaggc ggtttttccg ccggcctggg cccgatcatc tcatcatgtg
     5041 ccatccgggc tatgccgatg aggagctcgc ccgcatcgac accctcgtcg cccgcagaga
     5101 ggtcgagctc gcggccctgt tcctcgattc cgacctgccc gcccgaattc gccatccctc
     5161 acgcgacgcc atgggtcaca tctcgtgggg tgccgggaat gcctgaatcg ccctggccga
     5221 ccaaacgggc cggcgcggtc aggccactcg tccattactc cggatttctc atcgctggcc
     5281 tgctggcgct ggcggcggat ctgctcatcc tcgaagccct gatcctcgtt gggctgcatc
     5341 cgctgatcgg ccgcgttttt gcgatctcga tcgcgatggt ggtctcctgg ctcatcaatc
     5401 gcaccatcac gttcgccatg ccggggccgc cgagcttgcg cgagtatctg cgctttgccg
     5461 ccgtgtcctg gacctcccag gcggtcaact actcgatttt cggcgcaatt ctgatcttgc
     5521 gcccgtcggt gtcgcctctg ctggccgtcg tgctggcctc gatggtgtcg atgtttgtct
     5581 cctacttcgg cttccgatat ggggtcttca cccgcggacc cgccatggcg cgtccggcca
     5641 agccgcggac cgagaaatga gcagtctatc ggccgataca gtccccatca tctgtggggc
     5701 ggcggagctg tttgccacct acgatgtcgt gttctgcgat gtctggggcg tactgcacaa
     5761 cgggatcgaa gcttacgccg acgccgggaa ggcgctggcc cggttccgcg cgcaaggcgg
     5821 gacggtcatc ctcgtttcga actcgccggc ttcctcctcc tcgctcgcga ggttgctcga
     5881 ccggatcggc gtacgccggc atgcgtggga cgcggcgata acttcgggcg acctcacacg
     5941 cagccatatc acccagcgcg gctaccggct gatccaccac atcggacccg agcgggatca
     6001 gcgcgtcttt gcaggtctcg acgtcagccc cgtggctctc gaacgcgctg cggcgatcgt
     6061 ttgcaccggt gttgtcgatg accggaggga agcgggcgaa agctaccgtc cgctcctctc
     6121 caaggccctc ctgcggcgca tgccgttcgt atgcggcaac cccgatctgg tcgtcgacgt
     6181 aggtggcgag ctgctgccgt gcgcgggctc ggtggccacc atctacgaag cgatgggtgg
     6241 cgacgtctac tgggccggca agccgcatca gcccgcctac gaggcggcgc ggcagcttgc
     6301 ggagcagatc cgcggccggc cggttgacaa ggaccggatt ctcgccatcg gcgatgcgct
     6361 gcgcacggac ctggccggcg cggcagatta tggtatcgac tgcctgctgg tggcacacgg
     6421 cattcatcgc gacgaggtga tgagggccgg ccgcatagat cccgtaaggc tcgagaaact
     6481 gctgcggaca agcccccacc ggccgatcgc ggtcatccca gcgctggcgc attgagccaa
     6541 gcgcagcctg catcggccct cgcgcgttta tgctccgcgc ctcactattc attcgcgtgg
     6601 cgcgggatat tgaccggtgg caagatgccg tcgacgacac tgcaggagcc ctcgttcacc
     6661 ttcggcatcg aggaggaata tcacctcgtc gatcggatca cgcgcgacct cacgcctgct
     6721 ccgccggagc tgatggatgc ctgcctcgcc cgcctcggtg gccaggtcag ccccgagttc
     6781 ctgaaatcgc agatcgaaat cggcacgcca gcctgccgca cgtttcggga ggcgcgccgg
     6841 catctggccg ccttgcgcgg cacgatcggg gagatcgcaa ccggctttgg atgtgccccc
     6901 atcgccgcct cgacgcatcc gttcgctcac cacctcgacc agaagacgac ggaaagggag
     6961 cggtaccaga acctcgccca ggaaatgcag ggagtcggcc gccgcctgat ggtctgcggc
     7021 atgcacgtgc acgtggcgat cgaggattac gagttgcgca tcgacctcat gaaccaggtg
     7081 cgctacttcc tccctcatct gctcgcgctg tcggcgtcat ccccgttctg gcagggacag
     7141 gataccggcc tgaagagcta ccggctgacc gtcatgcagg cgctgccgcg gaccggcatg
     7201 ccaggccgtt tttccgggcg agccgagtat gagcggacgg tcgacgttct cgtgaaggcc
     7261 ggcgtgatcg aggatgcgac caagatctgg tgggacttga ggccgtcgca tcggtttccg
     7321 accctcgagg agcgcatcac ggacgtgtgc acccggctcg atgatgcctt gtccgtcgcg
     7381 gcgctctatg tctgcatctg ccggatgctg taccggcagc gccgtggcaa cctcacttgg
     7441 cgtgcctatc cggtttttct gctcaacgag aaccgctggc gggcgcagcg gtatggcgtg
     7501 cagggcacgc tgttcgacct cggcaagggt accttggcac cgttcacgga cctgctcggc
     7561 gaaatcctga cactgatcgc agaggacgca gcagcgctcg ggtgcgaggc ggaggtggcg
     7621 cacgcctccg agatcgccgc cgaggggacc agcgccgacc gccaggtcgc gacctacgaa
     7681 cggctgcgtg cctcgggagc gagcgacgag gaggcattga agggcgtcgt cgacatgctc
     7741 atccacgaaa ccctggccgg cacgcgcgtg cctccgccct tgcaggggcc gcccgccggg
     7801 atgagctagc gtccccctgc cagcattccc gcagagacgg atcaatcctt cagcaggaac
     7861 tccgcttcga tcgtgatcca gatgtcgtcg gaaatgagcg gcagagcgcg ggtgaggccc
     7921 caatcgctcc gctggacctt ggccgatgcc gagaagcccg aaacccacct gcctttatag
     7981 gtcgggttga cggcggcgaa cggatgctcg ccggtgaagt tccagatgac cttgagcgta
     8041 actggcttgg agatccccat gatggtgagc tcgccatcga cctcgccggt cttctcgccg
     8101 gtacggcgca cccaagtgct cctgaaggtg atgagcggat accggtcggc gttgaagaag
     8161 ctctcgctct tcagattgtc atcgaactcc ttgaccccgg tccaaaggct cgccgtcttg
     8221 atcaccacat cgacgctgcc gccctccgga tccgtgggcg agaattgcag ccggccgtcg
     8281 aaatccaaga agcgacccga ctggcgggac agcccgagat gatcccagct gaagcggatc
     8341 gaggagttct tcttgtcgaa aacccaggtg tccgcaaccg ccgccggcga ccagcagagc
     8401 atcgcgacca gcaagagcgc gaccgggtac aaggtgcagc gcaatgctgt gtggtgaaat
     8461 gcgcgagcca tcggctggtg agctccaggg atgccagggc gccaatgaca tgacgaaagc
     8521 gcccgcgagg tacagggctc ctcaccagta caatgtgacc tgatgttggc gcagcaccgt
     8581 ggactaaagg ccggctgcca tcccgccgtc caccgccagc tcggcgcccg tcacgaaagc
     8641 gctgtcgtcc gaggcgaaga acagcgcggc gcgcgccacg tcatcgcccg taccgaagcg
     8701 ccgcagcggc gtctggtcga gcagcgcctt gttgatcact ggattgcgca gcacccgctc
     8761 ggtgagcgcg gtctcgatga agccaggggc gagtgcgttc acacgaatcc cgaacggcgc
     8821 atactcgaca gcaagcgccc gcgtgagcgc cgaaacggct ccctttgtcg ccgagtagcc
     8881 ggcaagctgc ctcagtccgc ggctcgccat gatcgacgac aggttgatca gcgaggcgtt
     8941 gcccgatttg cggagcagct cgaacccatc ccgggcaacc cggacgacgc cgtcgaggtt
     9001 cacctcgcgg atgcgcaccc agtccgcgtc cgtgagatgg cggaagtcac tgcgcacgtt
     9061 gagaccggca ttgttgacga gcacgtcgag tttcgcgtgc cgctgctcga cggcacggaa
     9121 catagccgtc acgtcctgcc cgcgcgagac gtcggccgtc agcgctgcgg cgctgccccc
     9181 agcctcgctg atgcccgcgg ccgccctttc cgccgcggtt ccgtcgacat ccgccagata
     9241 gacgaacgct ccctcggccg caaagcagcg ggcaatggca aggccgatcc ccgcgccggc
     9301 acccgtcacc agcgcgatcc gcccgtcaag ccgtcccata tcccatctcc tgcctcgccc
     9361 tgaccgcgtc ggcagaacgg atagaaagct gcaggccatc cggcaagtcc tcgcggaccg
     9421 tggaacttgt ccggcacgag ttgcaatagg aacccgaggc aggaaacaaa ggcgagcttt
     9481 gctgtgcact attgcggtgc aataatcgcc ggggcaggat gatccgaggt tggaaggacg
     9541 caggaagggc agtcatgtcg acgaccacga gcagtacggc gagcaatgtg ggtgcttccg
     9601 gagctgaggc cgttgcggca cccgctgtcc tgccggcgac gaaggacctc tacgagatcg
     9661 gagaaatccc tcccctcggc cacgtgcccc gccggatgta tgcctgggtc atccgcaagg
     9721 agcggcacgg ggagcctgac agcgccatgc aggtggaggt cgttcccacc tgggagctcg
     9781 acagtcacga cgtgctggtg ctcgtgatgg cggcgggcgt caactacaac ggcgtctggg
     9841 ccgctctcgg caagccgatc tcaacgctcg atgtgcacaa gcacccgtac cacatcgccg
     9901 gctctgatgc ggcgggcatc gtttgggccg tgggctccaa ggtcaagcgc tggaaggtcg
     9961 gcgacgaggt ggtcatccac tgcaaccagg acgatggcga cgacgagcag tgcaacggcg
    10021 gcgatcccat gttctcgcct tcgcagcgga tctggggcta cgagacgccg gatggctcgt
    10081 tcgcccaatt cgccagggtg caggaccgcc agctgatgga gcgcccgcgg cacctgacct
    10141 gggaggaaag cgcctgctac acgctgactc tcgccacggc ctatcgcatg ctgttcggcc
    10201 atcgccccaa tatcctgagg cccgggcaca atgtcctcgt gtggggtgcc tcgggcggac
    10261 tcggctcgat ggccgttcag ctctgtgcca cggcgggcgc caacgcgatc ggcgtcgtat
    10321 cggacgacaa caagcgagac ttcgtcatgc agctcggcgc caagggcgtc atcaaccgca
    10381 agaacttcaa ctgctggggc gaaatgccga aggtcggcac gccggagtat gacgcctggt
    10441 tcaaggaggt tcgcaacttc ggcaaggcga tctgggacat caccggcaag ggcaataatg
    10501 tcgatttcgt cttcgagcac ccgggccagg cgacattccc cgtctcggtc ttcgtggtga
    10561 agcgcggcgg catggtggtc atctgcgccg gaaccaccgg ctacaatctg accatggatg
    10621 cccgctacct ctggatgcat cagaagcgcg tccagggctc gcacttcgcc aacctgctgc
    10681 aggcgtccca ggcgaaccgc ctcgtcatag agcggcggat cgatccttgc atgtccgagg
    10741 tctttgcctg ggagcagatt ccgaaggccc acatgcgcat gatgcgcaat gagcataagc
    10801 cggggaacat ggcggtgctc gtcaatgcac cggcgacggg cctcaggacg ttcgaggatg
    10861 tgctcgaagc ctcgcgtacc cgcttcggtt gaattggaag ctgaaagttt gtggttcgtt
    10921 aaccttcggt cgcgatcgtt cagggtgcca tcgcttgcca tggctgcatg tgatgtgacc
    10981 taatcgggcc actcgcagtc atccgcgtta ggaggacacc ttggcaaagg atcccgccag
    11041 tccgttccgg acgagagtgc gcgtcaaagg cgtggccacc gaggcggtgc cgtccgaccc
    11101 ggccgcccac gagacggcac ctgcggatag ccggctctcc ctgatggagg ccttgaagcg
    11161 cgccaaaccg ccaccgccct ccgggaaaga ccagcaaccg gaagtagagc gctccgaaag
    11221 ccagcacgcc ccgcggcccg ctccagtcgt tgcgcgatgg aagcaggagc agaccgatgc
    11281 cgagccaccg cgtcgcgtcc gccgcccctc ccggcgcgtc gggcggctgg ccgccgccaa
    11341 gttgcgccag ctcgcgcgcc tgaaagaggc cgcgtccagc ctcgaaagcc tggcgcgtgg
    11401 cgagttcgag gaagccaccg tcgaaatcgt ccgtcgggac cggaagacgt tggcgacgga
    11461 cagccgcacc cgcgattgac ggcctggggc gattttgact ctcgaaaccg cgcgccgcct
    11521 gcatcgttgg agagccaatt gcggcgcagc tcttagccat gttgcactgc aatctaatcc
    11581 ggtatgatct cgtcgaacga gccggcacgc cattgccgag ccaagcgatg aggcagcgga
    11641 tgggatagcc catgaggtgc cactcatcgc cataggctcg gagctcggct ctccagatca
    11701 ggaccatccg atgacgaacg cagcacctcc agccacgtac ccatccacca cagttcttgc
    11761 agaggccggc agcgtaatct cagctgccga acgggtgctg caggctgcga aggcttccat
    11821 gcagggcaag ctcgcggcat cgggtagtct cgacgattgc cagcacgctg cccatagcct
    11881 ctcctggctg gcaacgtacg tggaggcgat gcgccaactc ggcggctggg ccaatcggct
    11941 ggctgaggag cgcgccctcg gcgagctgga gcagctcctc cttaccattg gacttgccga
    12001 gtacgccgcc cagatcgccg gcggcatccc catgagccaa ggtgagttcg cacggctgaa
    12061 agacatcggc gtcgagacgg tcgaaatccg ccgcttcgag gatgccatcg cggatttcgt
    12121 tgcggagggc accggcgacg gagtgaaacg gcgcttggcg gagctcattg cggagctccc
    12181 gccgggcgag gtcatcggca atgcggggct cgacgaaacg catacggcca tccaggagca
    12241 gatgcacaga ttctgcgaag ccgaggtgac gccgcatgcc caaagctggc atctcacgaa
    12301 ttcctacatt cccctggaga cgatcgagaa gctgagcgaa ttgggcgtgt tcggcatcac
    12361 gctgccggag gactacggtg gcctcgggct cgggaaggag gccatgtgcg tcgtctccga
    12421 ggaactgtcg cgaggctata tcggcgtggg ctcgctcggc acccgctcgg agatcgcggg
    12481 cgaattgatc ctcgggagcg gcacggaaga gcagaagcaa aagtacctgc cgaagatcgc
    12541 aagtggcgaa atcctgccga cggccgtttt cacggagccg gataccggct cggacctcgc
    12601 atcgctgaag acacgcgcgg tgcgagaggg ggacgtttac aaggtgaccg gccagaagac
    12661 atggatcacc catcccgtgc gtgccgacct gatgacgttg ctcgtgcgca ccgatccgaa
    12721 tgagcccggc tacaagggct tgtcgatgct gcttgccgag aaaccacgcg gcacggatga
    12781 cgatcccttt cccgctcccg ggatgagcgg tggagagatc gaggtgctcg gctaccgcgg
    12841 catgaaggag ttcgacatct ccttcgacgg cttcgaggtg cctgtctccc agattctcgg
    12901 cggcgtcgaa ggccagggct tcaagcagct catggcgacg ttcgagtcgg ctcgcatcca
    12961 gactgcggcc cgcgcggtcg gcgtggcgca gtcggcgctc gacctggcgc tcgcctatgc
    13021 ccgcgagcgg gtgcagttcg gacggccgat ctatgatttt ccgcgtgtcg cgaacaagat
    13081 cgtgatgatg gccgtggagg tgatgatcgc ccgccagctc acctatttcg ccgcccgcga
    13141 gaaggacctg ggccgccgct gcgacctcga ggccggcatg gccaagctgc tagccgcccg
    13201 cgtggcctgg gcggccgccg acaacgccct ccagatccat ggcggcaatg gcttcgcgct
    13261 cgagtacccc atctcgcgcg tgctctgcga cgcccgtatt ctgaacatct tcgagggggc
    13321 ggcggagatt caggcgcagg tgatcgcgcg gcggctgctg gaaggggcga attgatccta
    13381 cccctgcatc ggttctcccg cactgtgggc gagaggcctg gtcggcagcc gttgaccact
    13441 gccgccccac catgttccat cgtcgaaagc atcccatcag agcgagatcc tagcaatggc
    13501 cacaccgtct tccgatcccg tcgtcatcgt ctccgccgcg cgtacgccgg tcggtgggtt
    13561 tcagggtgca ttcgcgtccg ttgccgcgcc tgagctgggg gccgtcgcga tcaaagctgc
    13621 cgtggagaaa tccggggtcg agccggagga ggtgagcgag gtgctgatgg gctgcgtgct
    13681 gccggctggc atcggtcagg ccccggcgcg gcaagcatcc cgcggggccg ggttgccgga
    13741 ccacgtcccc tgcaccacgg tcaacaagat gtgcggctca ggcatgaaga cggtcatgat
    13801 ggcctacgag agcctgcacg cccggccaaa cgacatcatt ctcgccggcg gtatggagag
    13861 catgacgaac gcgccgtacc tcttgccaaa aatgcgctca ggccagcggc tcggtcatgg
    13921 gcaggtctac gaccacatgt tcctcgacgg tctggaagac gcctacgaca agggccggct
    13981 gatgggcacc tatgcggagg acacggctca ggcctaccag ttcacccgcg aggcccagga
    14041 cgcgttcgcc ctcgaaagcc tgaagcgcgc gcggagggcc aacgaggacg gctcgttcgc
    14101 caaggagatc gtcccggtta cggtgaaaag ccgcaagggc gcagtcgaag tgacccgcga
    14161 cgagcaaccg ttcaccgccg acccggccaa gatcccgcag ctgaaacccg cgttccggga
    14221 gggcggcacg gtaacaccag ccaactccag ctcgatttcg gacggcgcgg cagccctcct
    14281 cctcatgcgc gaaagcgaag ccaatcgccg taaactgaac cctctcgcac gaatcgtagg
    14341 cacggccacc catgcccagg cccccgcctg gttcacgacc gcgccgatcg gtgccatcag
    14401 caagctgctc gagatggtcg gctggcgggc gcaagacgtc ggcctcttcg aggtgaacga
    14461 agccttcgcc gtggtcacga tggccgccat gcgcgatctc aatctgcctc atgacaaggt
    14521 gaacgtgcac ggcggcgcgt gtgcgctggg gcacccaatc ggagcctcgg gcgcgcggat
    14581 catcgtcacg ctcctgtccg cgatggaaaa atacggcgag acgaaaggcg tggcgtcgct
    14641 gtgcatcggc ggtggcgagg cgacggccat cgcgctggag cgcgttgtct gaagcctgcg
    14701 gctagagcag cttcggccga tgcacgtcct ttcgcgcgtc ctgttctttc tggtgcaacc
    14761 ctcgcatttt gcgctgattc ttgtcggggc cgggcttttg ctgctcttca tccgtcccgc
    14821 cgcagcacgg actggcaaaa tgcttgccgc aacaggattt ctatcgctgg tcgtgctcgg
    14881 cttctcgccg atcggtcacg cgctactgct gccgctcgag gaacgctttc cgcagggacc
    14941 gatgcccgcc gggaaggttg ccggcatcat catgctcggg ggcttcgagg acccgggcgt
    15001 cagccaggcg cgcggcgcac ttgcgctcaa cgaatcggcg gaacgcctca tggcaccgat
    15061 attgctcgcc cggaaatttc cagaggcacg catcgtattc accggtgggg cgtcagatat
    15121 ctttctgagg agcgacagcg cggccccttt cgtcgccacc tttcttacag cagcaggtgt
    15181 agcgccggac cgcatcgtgc tcgagggcaa gtcgcgcaat acttacgaga acgccatcta
    15241 tacgcggtcg cttgtcgatt ccgggcctgg cgaacgctgg ctgctggtga cttcggcctg
    15301 gcacatgccg cgtgccgtgg gtgcgttccg gcgggccggc ttcgacgtcg tcccttggcc
    15361 caccgactac cggactcgcg gctcggcaga tctgcttcag ccgttcgata aaatgactga
    15421 aggattgcgg cgaacggata tggccgtccg cgagtggatc gggcttgccg cctattggct
    15481 tctcgggcgg tcggattccc tgttccctgg gccggcgccc aatcccgcgt gaacgaacga
    15541 ctttttatct cttttccagc tttggcgact cggctgccgg ctttggctgc agctccttga
    15601 cgagatcgaa cgacttgcag cccttctccg ccagacgacg gttgtatgcc tcgaccattg
    15661 cgcgatcgcg acggtagtcg gcatcgggat cggcaccccg ctcggtgccg ccgaaaacgg
    15721 gcgtcacgac cttctggagg ccccgggcag cgagcgagcc tttccgggtc aactcgtaat
    15781 cgcggatctg caatatccga accagtgtcc ggcccgccag cttgtggcag ttgagcgcca
    15841 gctcggaagc gctgaggacg tagctgccat ccggctgcaa accggcctga gcagggctct
    15901 ctgcagccgg cctttgctcc gaggccgatg attggtcaat cgcattggac tgcgcgcccg
    15961 cgcatccgca gacgaggccg gcggccaaca atacgatctg tacttcaggt ttgaacatga
    16021 aataggtgtg ccccgacttc ccggccgagc tgatgaccct aaccgggcag atctgcggca
    16081 aacctctctt gaagcgtaag ctccggcccg atatcaacag gagatcgacc aagatcatgg
    16141 gtgaggaaag tccgatgccg caaaagataa tgatgggcgc gaaggcactg gtcgcggcgg
    16201 cgaaagcgga gattaccgag tacagcgctg gcgaggcgat cgagctcagc aaacggccgg
    16261 acgtgctcct ggtcgacatc cgtgacccgc gggagctcgc gcgcgaaggc aaaatcccgg
    16321 gcgcctttca ctgcccgcgc ggcatgctcg aattctggat cgatcccgag agcccctatt
    16381 tcaagcccgt attcggcgaa gaccggacgt ttattttctt ctgtgccggc ggaatgcgct
    16441 ctgcgctcgc gaccaaaact gcccaggaca tgggattgaa gccggttgcg cacatcatcg
    16501 gcggcttcgg cgcctggcgc tcagcgggcg ggccgatcga gggacccgct accgaccgtt
    16561 agctgccccg gcggccggct gcacatcgca gttcatcagt ggagggcgtg agagccgtcc
    16621 ttcagcaagt cgcgcagcaa ctcatccagc aattgtgtcg cgagcgcatg ggcggtcggt
    16681 ggatcgaggt gctcggccag caatctcatg cccgtcatga tcgcggcgcc ggcctcctcg
    16741 atctcctcgg tatcgtgaaa aggttggttc aacatcgcac tgtcctcgtt tgcgatggct
    16801 cgttcgttag cgaaaacgcc cgccaatccg aaacgatcca tcgcgaattc cgcaggcaat
    16861 cgcttgagca acacccgtgg aactgtgcga cttcaattat caattaatat tgatgattgt
    16921 cggaatgcca tccggctatc cgctgcgcga aacgatttac ctaagatatt tcggacaggt
    16981 gcctaccctc caactaccgt gccgccgttc ggatgcagca cctgccccgt catgtagctg
    17041 gaatcctcgc tcgccagaaa cacgtagcag ggagcaacct cgcgcggttg acccgcgcgg
    17101 cccatgggcg tgttctttcc gaagctctcg atgccctcgg cggggtaaga agaagccacc
    17161 agcggtgtcc agatgggacc tggagcaacg ccgttgacgc gaataccacg atcgacgacg
    17221 gcctgcgaca gcgatcgcgt gaacgaaacg attgcgccgc gtgtggcgct gtaatccatc
    17281 agatgcgcgc tgcccttata agccgttatc gacgtcgtat tgataatcga agacccctcc
    17341 cgcaaatgag gcaacgcagc tttcgtcata taaaagtaac caaaaacatt gctccggaat
    17401 gtccgctcga gctgctcggc tgaaatatcc tccagccgat ttctaaggtg ctgctcggct
    17461 gcgttgttca cgagaatatc gagtttcccg aacgtatcga ccgtccgccg caccacatct
    17521 tggcagaagg cttcatcacc gatatccccc ttgatcgcga gcgcctgttt gttctccgcc
    17581 tcgatgagct gtacggtgtc gtcggcgtcc cgttgctcct cgaggtaaac aaacgcaatg
    17641 tcggctcctt cccgcgccat tgctatggcg accgcgcgcc cgatgccgct gtcgcccccc
    17701 gtgatcaggg ctatcctgtc ttcaagccgc ccgctccctt tgaaccgcgg aacgtattcc
    17761 ggtgccgggt gcatcttgtg ctcgtggcct ggctggggct gttgtgcttt ggggggcgtg
    17821 tccgcactca tggcgatttt tgtccgttag aaattttcca gtaagggcag acaaccctag
    17881 caagccgcgg caagttccag atcgtctgcc cgccgctggg aagcgctctc aacttcagtt
    17941 caccagatca ggtttctcgc cacctgtttc cgccgcgtcc tcagagagca ggatcaccag
    18001 ctggcgcata cagaagcggt aacgcccttc tctcaaccga agtggcgctt gcgatatggc
    18061 gcgcagaacg cattcttccg aaactagccc gcgtgcggga tggaattcgg ccatggcggg
    18121 gcagctcatc aactccacgt acgcgtcgag ccgcgcctga atggcaatgc ccgtttcgac
    18181 gatcccctcg actacgcctt catcgatcag catgtcgagg atggcttccg cccggccgcg
    18241 taaagtgacg gcctgagcgg gctcgagccg aaggacggct tggacggcct tggcgagaag
    18301 ctcaggcgag acagcggttt cggttattgc gtcacgctct tccatcgtcg caactgaacc
    18361 cggcatcggc atccaggttc cgctgcttat cggcggcaga cgcctcgctc aggcgctcgc
    18421 aaccgcggcc aaccgcagtt ttgcgaattg gtcgccggga acatcgagcg tccacagcag
    18481 gccgtcggga atggccttca gaacgacgtt cgcgccgaga gccgtggggg tcacatgctc
    18541 gaggatatcc cgcccgaagc ctcgatgcag caggtcgccg gcagactgac ggccgcgctc
    18601 gcgccagacg atcaagaggc gtggctcatc ccctttccca cgctcgacgg accagcttag
    18661 gtctacgcga cccgtcgcca ccgaaagggc gccatgccgt tcggcattgc tggcaagctc
    18721 gtgcagcgcg aggccgatgt tgaggatggc ctcggggcga aggaacacat cggggcccga
    18781 agcgcggata cgcgaatgcc ggcttccgaa tgcgctccgc tgcacggcga cgagatcgcc
    18841 cagcaaagcg ccttcccagt cgttgcttat gagcagctcg tgagagcgcg acagactttg
    18901 caaacgccct gaaaagcgct gctcgaaatc ggcgagcccc tcgctgcgca gggcggtctg
    18961 ccgggccatg gcctggatga tcgccaacaa attcttggat cgatgcgtca gctcgcgcag
    19021 gacgctggcg agatggcgat cgtgctcctt gcgcaccgag atatctcgga tagtgatcga
    19081 gtagccggca atgtcgcccg aatggccgta caccggattg gcgatgatgc tgacctccat
    19141 ggtatgcccg tacttggtgc ggcacacgct ttcgagccgg accgtctcgc ccttcgccat
    19201 tgccgcgctg atacgctcca tgccgctcga atgttcgtcc agaaagagat cctgcagcgg
    19261 ccggcccaca gcctccgcgc tgctgtagcc gaacgtgttc gcggcacccg tattccaagt
    19321 cgcaatgatg ccgtccggcg aggcgctcag gattgcctcg ctcgaggaag caacgattgc
    19381 cgccagataa gcgttggcgt tctcggcttt cttgcgttcc gtgatgtcga tgacgacgcc
    19441 caggaccgaa atcgctacgc cgtcagaatt gcgcctgacg aatgcgcggc gatagagcca
    19501 gcgctcttcg ccggacggca ggacgatcct gaactcctga tcgaaagtgt cagccgggcc
    19561 gcggatcgcg tcctggtgag cctgacgaaa gcgcacgcga tcgtcgggat gcacatagct
    19621 gaccacctga tcgggatcgc ccgagaactc gtcgaccgag cgcccgaaga ggcgatacat
    19681 cgcctccgac caagtcacat ggttggtcgc gagatcgcgc tcccacatgc ccatgtcggt
    19741 agcggacagg gcgagcgaca tgcgctcgtg cgcaatcctg agctgatctt cggcaagtcg
    19801 ccgctggacc gacgccgctc gcaatgcact caggacttca tctacctcac ggaggcgcaa
    19861 ggccaactcg ggcatcgcct cgccctttcc gaaggccgac gccgcgagcg cagtcatccg
    19921 gatcggcgag gcaagcctgg agccgaaata gagtgcggcc aggagcgaca ggctgagcaa
    19981 ggcgccgccg ccgagcgcga ggtactgcca tgaccaaagc cctggcaata ggacgtcgag
    20041 cggagcgctc gccatgacca gccagcttgc aatggtggag cgggcaacag cctggaattc
    20101 atcgcggccg aaagcgctta caagctcgac ccgatcagcc tgatcggcac ggattcttgg
    20161 tggcattttg gccgaatcga gctcgaatcc ggcggagtgc gcaatgagac gaccctgtcc
    20221 atcgatgatg ccgtattccc agccgtcgct cacgggcgca ctttggaaaa gcttggagag
    20281 gcgagacgaa tcgaacgcag ccgtgatgag agcgctcggc tcgttctttc tcagcaccgg
    20341 caccgttatc gtaaagctgg cctcacctga agcgaggccc gagacataag gctccttggt
    20401 ccggacgaca ttccggaact ccgtgctcgc gctctcggag gcgggcagcg ccccacccca
    20461 ctggacccgc gtgtcgacca gcggctgccc ggacagatcc gacagaacga tgtacagatc
    20521 ggacccttcg cggacgagct ttgacgcgaa attgtggaac gccgccaagt cccctctatc
    20581 gaggctcgga gaggctgcca gtgtctgcaa agcgagaata acgctgctga gctcccgatc
    20641 gacatccgac ttcagtagtc gaacgatctg cagcgattgc tgctcgagtc gctccctttc
    20701 ggcagcaaca ttcttatgca gagcaattcc ggaaaggacc aggatcggcg cggcaacagc
    20761 cagggcgtaa acggccagat attctccgat cgtccgaggc cgcggccgag ggcgacgggt
    20821 gcccgcaaat ggccagcttt ccagcacggg tatgtatgcc atttccccct cttttttcga
    20881 gtgtcacccg gtccgtctga agacctggca gcgactcaaa ccccgtaggt tgtgcagagg
    20941 catacgcacc acagaaccga tacccacagc tcggcgcggc aagtggcgag gcgcagactt
    21001 atagtctgtc gcgcacatgg atgccgcctc tccggagatc accggattcg gctgccagcc
    21061 gcgtggtact tcgctgagct caggtaccgc gcgcacgctg tcgtcctcgc agcacggctc
    21121 ggccgctggc gtgtgtcgag gcagtgttgc acgcaaagtc aaggcattaa ccctctcaac
    21181 ggaagctaac gcgcaaccga cttaaaggtt ccatttcttg gcattactgg ttctctcgtc
    21241 ctacttcgat ttcaaaaccg aaacatttgt gaagagtcct ccgcgggctg gaacggtttc
    21301 cgctttgtac gcgtttggtt tgtgccccta gacgtggacg caggcgatgc gtccagttcc
    21361 tgaccgcggc tacaaaggta gccgcggtcc ttttttcatc ctgagcctaa gcctaatgaa
    21421 aactgaacgt cggattgtgc agccgcgcgg cgcgacagcg gtcctgtcct tgctctctgg
    21481 ccgagggcaa gaacgcacta gcgccgtctg cgttccttcc tcgagggaac tcgcccagcc
    21541 tgccctcgcc ctttgagcga ccaaggaccc attctgatgg aactgagccg gtacaagcgt
    21601 gcgagagcgg cagctgctcc ttccttgccg ggctcgctgt tgacggcggc gctggtcatc
    21661 gccggtctct acttcggcag cgacctattc gttccgcttg caatcgccgt gctgctgagc
    21721 ttcgtgctga cgccattggt ggttgggctg cgacgatttc gcatcccccg cgccatcgcc
    21781 gtcgttctgg tcgtggtttc agcgttctcg atcatcttcg ggctcggcgc actcatgggc
    21841 ggtcaagtca ccgcgctgct cgaacgcctt ccccagtacg aatggacgct gcgccagaag
    21901 atcagagacc tgcggccgca gcctggcgca cctgcaggca tcgtcgagcg gacgaccgaa
    21961 acgctccaaa atttgggcaa ggagctcgac gagcggtcgg aatccccatc tctggcgcag
    22021 cccaaaacat ctgccggtcc gcagcccatt ccggtcgaga tccatagccc gccgccggcc
    22081 gcgctggagt acttccagcg gatcgtgcag ccgttgctgg agccgctggc gagcacggga
    22141 ctggtcttga ttctcgtcat cttcatcctg cttcagcgcg aggaccttcg tgaccgtctg
    22201 atccgcctat tcggggtcgc ggatctagga cggaccaccg atgccatcga cgacgcggcg
    22261 cgccggctca gccgcttgta tctgacgctg accaccatga acgcggttta tggcgcaggg
    22321 atcgcggtgg cgctgtggct gatcgggctt cccaatcccc tcttgtgggg catcctggcc
    22381 ggccttttgc gttttgtccc ctacatcggc accgtaatcg cgttggtttt tccggtgctt
    22441 cttgccgctg ccgtcgagcc gggctggtcg cttgcggcat taactttgat cattttcatc
    22501 gtcggcgaat ttacgatggg gcaggtgttg gagccctggc ttctcgggaa ctcgacgggg
    22561 ctctcgccgc tagcggtgat cgcggcggca tcgttctgga catggctgtg ggggccgatc
    22621 ggcctgctgc tcgcaatccc gctcacggtt tcccttatgg tgctcggccg gcatgtgccg
    22681 cagctcaact tcatctatgt gctcctcggc gacgagccgg ccctgaccgc ggcacaacgc
    22741 ttctatcagc gcatgctcgc cggagacatc gacgagatca cctacgatgc ggagcagttc
    22801 gtcaaatcga agtcgctcct ctgttatttc gacgaggtgg cgctccccgc gctcgtcatg
    22861 gctcaggctg atgtccggcg ggagcggctt gggccagacc ggctgtctga aatgcgggag
    22921 ctcatcggtg agctgatcga ggaactcgag gatgtcgaaa tcggcaagcc gccgccgaag
    22981 gcgggcaagg aagacgagga gcagccttcg gacttgccgg tgctcgcgcc ggaagaattg
    23041 aaacccggct ggcgcgtggc cgagccggtg ctgtcggtgg gcgtgcgcaa tccgctcgac
    23101 caggcggcag cggaaatcct ggcgcacctt gccgccggct acggcgtcgg aacccgggtg
    23161 atcggcgcaa gcgaagtgac gaccgcagcc atcaatcaac tcgacatcgc cgacgcaaaa
    23221 ctcgccgtgt tgtccaatgt ggacacggcg cgtgctccag cccatgcccg gctgatgatg
    23281 cgtcggctca ggcggcgcaa tccaaacctg aagttcctga tcggggcctg gggcgaaagc
    23341 gaagccgggt ccgggccgga ggcggtcgcg ccgatggaaa gcggtcccgc catacagcgg
    23401 atgacgtcgc tgcggtccgc actcggggag attttgagag aagcccgggc ggagccgtca
    23461 agtgtcccga taccggccag gaaagtcgag cagccggagc cttgcagcgt gggcgagctg
    23521 cagccggagc ccgctgctca acgccggcca gcgtagggat tctcgccttt ccgcagcatg
    23581 aagcggatcg gaacgcccgg cagatcgaac gtctcgcgga ttccgttaac gaggtagcgc
    23641 acataagcct tcggcagccc cttgggttgc gaggcgaacg cgacgaaggt gggcggccgc
    23701 gtcgagacct gggcgatata gcggatcttg atccgccggc ccttcgatgc agggggcgca
    23761 tgttgctcca cggcttcccg cagccagcgg ttgagatccg gagttggcac ccgccggttc
    23821 caggcctcgt agacctgcag tacggctgcg agcagcctgt ccagcccgcg ctctgccagc
    23881 gccgatatgg ggaccaccgg cacgcccggg acctgcgcaa gcttctccgc aacggactct
    23941 cgcagatcgc gcagcaccgc ctgtttgttc tcgatcaggt cccacttgtt gacggcaatc
    24001 acgagcgccc gtccctcttc cgccacaaga tcaccgatcg tcagatcctg atgctcgagc
    24061 gggcgctcgg cgtcgatcat cagaatgacc acctcggcat agcggatggc gcgcatggtg
    24121 tccgacgtcg cgagcttctc cgccagttcc gtgatgcgcg ccttgcgcct gaggcccgcc
    24181 gtgtcgatga ggctcagctt gcgcccgcgc cattcgaaat ccgtggcgat ggcatcacgc
    24241 gtgaggcccg gttcaggccc ggtcaagaga cgttcctcgc cgagcagcgc gttgaccagc
    24301 gtcgacttgc ccgaattggg acgcccgacg atggcgaccc tgatggggcg cgcgtcggca
    24361 tcttcggctt cgatctgctc ctcggctcgt gaacccttct ttgccggtgc ccaatcttcg
    24421 agccccagcg ccctcagcat ctccgaaacg agctcgccga tgccctcgcc atgctcagcc
    24481 gagatcgcga caggctcgcc gagcccaagc tcgaagctct cgtagaagcc gtctagcccc
    24541 ttgcggccct cgcatttgtt agcgaccagg atgcagggcc gccccgcagc gcgcacgacc
    24601 cgggcgaagg cggcgtcagc aggcgtgatg ccctcgcgcg cgtcgatcac gaacagggcc
    24661 agatcggcgc tcttcagcgc ctgctccgtc tgccgcgtca tgcgggcggc gatcgtcccc
    24721 ggcgctgctt cctcgagacc cgccgtgtcg accaggttca cttcatatgc gcccagcctc
    24781 gccgtgccct cgcgccggtc ccgcgtgaga cccggcatgt cggacacgag cgccgcacgc
    24841 cggccgatga gccggttgaa caacgtcgac ttgccgacat tgggacggcc gatgatgacg
    24901 atgttgggtc tggacgtcat ggggcgggct gccagtaggg gaaatcctgc cggccgtcga
    24961 ggccaaggcg agtgaaattg atcgaagcga aactgccggt cgttagttgt aggcgacgag
    25021 ccgcgcctcg tccgtcagca cgtacatgcg cccgccggcg acaatcggtg caatgtaaac
    25081 gggtccgccg acgccctgct ggaccgtgac tcgcccggtc gctgggtcaa cgccgacgag
    25141 ctgacctttg ttcgaggtga gccagagctg attgcccgcg agcgtcgggc cggaccaggt
    25201 gctcgtgccc ggtagcttcg ccgaccagat caccttcccg tcctggcggg taagagcatg
    25261 gagctggccg ttcgtgtcga ccacgaagat gttgtttccg gcgataactg gcggctgcgt
    25321 gcccggcacg ttgagcgacc aaaggcgctc gcccgtcttg attcgggtgg cgatcaggcg
    25381 acccgcgtgg ccaactccat agaccacgcc gtctgcgatg acgggccggg ccgcatcgct
    25441 caacgatgcg agcgaggaca cggtccgcgt ccgcgcaagg ctttccgacc acacaggcgt
    25501 gccgtcctgc tcgtgcaatg cgacgagctc tcccgaagga tacggtacga tgaccacccc
    25561 cgcatcgaca gccgggctcg ggttcgaaat gatgctggta cgctcgggca agccgcggaa
    25621 ggtccataac tcgccgccgt cgaagcccgc cagcgcgaac acgtgtccct cggtcgtcac
    25681 gacgaagacc ttgtcgtcca ccgccgtcgg cgaggagcga atgggaacgc cgatgttctt
    25741 cgtccaaagc gttttaccgg tggcgggatc gagggcgacg acggtgccga agccggtcgc
    25801 gacgtaaagc cgcccattgt cgatcgcgag gccaccgccg tacccgctct cctcccgctc
    25861 gccctccggc accaacgagg tgcgccatac gaccgatcca ccagaggcag agaccgccgt
    25921 caccttcgcg gttgcgtcga gcgcaaatac gcgcccgccg taaacgaggg ggctcgccgt
    25981 gagcttgccc ttggtgctgg agccggagcc tacgtccgcg ctccaggccc gcttcacgga
    26041 accgccgagg gcgagatggc cgggcgcgtt gctgggtacg ccgccgggtt gggtccattc
    26101 ctcgttggcg cgcggggcag gcaacacgac cggcttgtca gccgtagcca gctccccgcc
    26161 gatcgtatcg cgcgcctgca tgacaggaat gcgcttgccc ggcagcggat gctctttttc
    26221 ggcgaacggg ttgaggtcgg tgatcttggg caggctcggc aggctactac cgccgcaacc
    26281 gccgaggccg atcgccacga gcaaggtggg cgccagtatt tgccccagcc gttgcgatcg
    26341 tttccccgcg atgcccatca agcctccccg tcactgcacc ttcggcgatt ctgcgggctt
    26401 accgccgctg accggctggg tcgaacccgt ggtctgctgc gcctccgccg aggtgatcag
    26461 gcccatcatg atgccggaac gctcccgtac cgaaggaggc gttttctggt cggcagcgag
    26521 ctccaggaaa atctgcctcg cttcctcaag acgacccgca cgataagcag acaggcccag
    26581 aagctcgcgt gcgctatgcc tccaggcatt gctgtcctgc atcagagggg tgagccgatt
    26641 ctgcatttcg gtaaagtcgg ccctgtcgat tatcaacgct gccgtttgca gccgggcata
    26701 gtcgcgaagc aaggggtcga cgctcccgtc ggcggctatc gcctcaaaac tcctgagtgc
    26761 ctcgtccgtc tgcccggccg atgccgcctc gccagcgagc tggaggcggg ccagggtggc
    26821 atagcccgat ggcgcattct gcaccaggtc ctggaacgct tgccgcgcct cgtcggcctt
    26881 gccttgctct acgagttgca tcgccgtctg gtagcgcgcg ccggcctccg cagcggcgga
    26941 aagggtgcgc gcttcccacc acttgtagcc ccccacgctg gcgacgatga gcaccgcgac
    27001 aacaatggcg tagatgccat agcggtccca gagcttcgcc atctgctcgc ggcgaacctc
    27061 gctttcgagc tcccgcaaca ttgcgtcgcg ttcgtcaacc attctcggat cggccctcct
    27121 tgcgcctgat cctggatgcc ttctgcgtcc agggcgctgc actcatagcg caatgcgccg
    27181 gtcacgccaa cgcgaccgct ttgcggccac aatcgcccgg gcggcaaacg ggcgagcggt
    27241 ttacctcctg gtgcaatggg tttccggcgg ttttgcggcc tgcattttcg ccgcggcccc
    27301 cctacgactg gcccggcagc agaggcacgc cgatcagcca ggcgtgcccg acagtcagca
    27361 tgaacccata aagcgcgagt cccgccgcga tagcgataat gtcgttgacc cagccgtggg
    27421 gcgctgccgg tatcttgaca ctgcggcgct tcaccgagat ccggtcatag acggcatacg
    27481 cgagaaaact tccgaagagc aggatcgagg caagatcgcc atttgcaagc agatgcgcgg
    27541 tcgcccacag cttgatgcct gccagcatcg gatgcttggt cgcagtgcgg atgtgggacg
    27601 gcacgtaggc ggcaacgatc agcaccatcg ctggtagcat caggagaaag gcaatgtgcc
    27661 gggtccagat cggcggcgtc cagatctccg ggttcttgcc ggccattacc tgcagcttgc
    27721 catagccaat gatgatcagg acgagaccga cgagcgcgac gaccgaaaag atcgccttgt
    27781 agcggttctc ccccaatcgt cctgccagtc cgcgcctcag gtccgcctcg gtcggaacga
    27841 ggtgcaccag aaagaacacg agtagtccga gaacgagcgg aagcatcgat cgactcctgc
    27901 cgcctataag ccgagttccg ttaaccgact gccaactttg tgcaccgact tgtcagtcct
    27961 gccacaactc cgacgaacct ttattcgatt gtgccaatca gcgcgagcat gttagttgac
    28021 gctggggacc ggggaagcgt gggggtccca ttggagattt gcgtaggctt gaccggcgtg
    28081 gcggcggcct tgggaatcgg tgcggatttg cgctccgaca tgcgcagctc tgcgagatcc
    28141 ccccaagcat cgggaacctg agtgaggtta cgatggacag cagtcgcgaa ggcgcggtca
    28201 agaagctcaa tggccatggc aatgggctga atggccacgg gactggacaa tccgacgacg
    28261 gcgcaccgca accgccgctt tccggactgg tggacgag
//
LOCUS       DASVDU010000007        11586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1530857, whole genome shotgun sequence.
ACCESSION   DASVDU010000007 DASVDU010000000
VERSION     DASVDU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11586
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530857"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF226_00500"
     CDS             complement(<1..209)
                     /locus_tag="VF226_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde dehydrogenase"
                     /protein_id="HEX7072498.1"
                     /translation="MAIYAREIEQGGQARIDEAVTALAWSLYKPENARRMAEIAVEVT
                     GIGEVESKVIKNQRKTFGTLRDLLRV"
     gene            533..1366
                     /locus_tag="VF226_00505"
     CDS             533..1366
                     /locus_tag="VF226_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7072499.1"
                     /translation="MRESGERGSALNKALAVLEAITAQSQPIGLPDLSARLALPRQTV
                     HRILVQLEEAGLIVRDPQRDRFSVGPRLSTLALNALQTHNQSAPIRAVLQQLVDDIEE
                     TCNVGVLDGLDFLYLERIECHWSLRVHLQAGSRVPAHCTSGGKVMLAHLDETVRAKLF
                     RNISLKAYTSTSITRVSDLEADLAQVRERGYAVNNQEFTVGIIGVAVPVADRHGRVLA
                     ALAVHGPAPRLDLDRAIGFVPRLREAATVMAEAWTGGDDAASTRSRAFARASKPVAPS
                     F"
     gene            1478..3232
                     /gene="xsc"
                     /locus_tag="VF226_00510"
     CDS             1478..3232
                     /gene="xsc"
                     /locus_tag="VF226_00510"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HEX7072500.1"
                     /translation="MTRMTPSEAFVETLVAHGVTDVFGIVGSAFMDALDLFPTAGIRF
                     IPTVHEQGAGHMADGYSRASGRHGVCIAQNGPGVTNFVTSTAAAYWAHSPVVVITPET
                     GSNTIGLGGFQETEQLPIFSKITKYQAHVSRQDRMAELTARCFDMAMSERGPTQLNIP
                     RDIFYGEADYEIPVPKKIARGPGADEALDEAAELLASAKFPVMVAGGGVIFSGGIDEA
                     KALAEYLQMPVVNSYLHNDSFPADHPLWCGPLGYQGSKAGMKIIAEADVVLALGTRLG
                     PFGTLPQHGLDYWPKNAKIIQVDTDHRMLGLVKQISVGIVGDAKAAARALHHRLANRD
                     PLARKNASERLQKIKAEKDAWEAELSEWTHERDPWSLEVSKNSPNMHPRQMLRELEKA
                     MPTSAMVSTDIGNICSVSNSYLRFNQAPSMFAAMSFGNCGYALPTIIGAKVARPDRPA
                     IAYVGDGAWGMSFGEILTCVRESIPVTAVVFHNEQWGAEKKNQVDFYNRRFEGVNLKN
                     PSWAEVARSLGAEAVTVRNLGDVGEALEKAVDAQMKHGKTTVLEMMVTQELGDPFRRD
                     ALKKPKRLLDKYKHTNVD"
     gene            complement(3381..4181)
                     /locus_tag="VF226_00515"
     CDS             complement(3381..4181)
                     /locus_tag="VF226_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7072501.1"
                     /translation="MMRFGMLLVALVACAGAANAADLGGIPYGSMKDEPILVAPPSWS
                     GLYFGAHAGWVTGGWDGNMAYSEAVPGDGFDRSGKSLDDDGFLGGLQIGWNRQHGAFV
                     WGLEADVSWADLNDRARLFPYPLNESPAWDIDNELELFGTVRGRLGYLVTPRLLLYGT
                     GGFAWGRANSDLTVVDTNTPPFTVVTAKGSADENHLGWVAGAGGEFLLADRWMVRAEW
                     LHVDLGEEDYHFVGRTSSGAAHVTDSFPASLEFDVFRVGLNYNFGTGQ"
     gene            4562..4654
                     /locus_tag="VF226_00520"
     tRNA            4562..4654
                     /locus_tag="VF226_00520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4596..4598,aa:Ser,seq:gct)
     gene            5105..6151
                     /locus_tag="VF226_00525"
     CDS             5105..6151
                     /locus_tag="VF226_00525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7072502.1"
                     /translation="MVSRSKVQLFAAAAAATTLVLTTLLPQQADAQERKSIRWATSSV
                     DSYGYKIAASMVKIAEEALGGEYTVTVNPYPSTTGAMKATMDGNGEISYTADVGMTQL
                     YNREGGFKSYQPSKGMLVHTWYAYPMETFMMVLADKASQYKCLKDIAGQPTFFTPAGY
                     MNWLNFQRVYKALGYDFKHVQIDPKTQADALKSGTIAGAAAYTTAGRSLTPYWKETEV
                     RIDVKAVNPCPDEIEKLKAAGLSISPVDPSVFTKDVGVKEIQGVPILFGYNVRADMPE
                     DVVYKLISAFYENRDELAKAEPGFTPMAKDFIGMQVSGIKSAPDVPVHPGLAKFLKEH
                     NAWDEKWKVAESKS"
     gene            6321..8339
                     /locus_tag="VF226_00530"
     CDS             6321..8339
                     /locus_tag="VF226_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX7072503.1"
                     /translation="MTAKDVKEFFNLTNIILFFGAIQLAWLTWYFYTGFGGPQELVAH
                     VMSITLILQILFMYRKDYFYKWLPPRVNDLIILFYIGICIYAFWHFWWEFEEIAIYRQ
                     GSYTTQDFIVGLLVFLLVMELSRLAHPVLFWVNVVLIFYTLWGYLSPVDFFWHPGTSF
                     YRIVTSSTVEFSTGIYGIYGQIALTLIAAFLLLAAVAQGFGAQNAMINVMRRLAGGSR
                     QMVPQTAVLGSLSIGMISGSGSANAVVVGSITIPLMKRYGLPGAFAGGVESAASMGGL
                     IMPPMMGIGAFLMSQFLGVPYWDVVVRGFSLAIVYYVTLALAVYLVCVRLLPSDNVPA
                     PQVPLYDRVKTSIFFLSVIFLIYELGIAGTGELLAALHAAGFMLALLLAAFLYFKYVL
                     KDPAVVEQSLIGNLKLSLETHADMTSYLTLLLATLGIMIGLFTVTGFINRMGGMLLDL
                     GAWNIMAMIFMAYLFGWLVGAGLPPTATYIIGAVVIVQPLVSLGINPWVAHFFVFLLS
                     VWGELSPPTSLTAAISSRIAEASFMRTMWEALKLCLPITLMTFAVFTRSEMVVTPGWT
                     QVLNTAAMLIGTCGIAFAIFGRYTRNSLADVALRLVLTLLAGAVLFHPNDTVALSLSP
                     LVLAGVILGILRHRIVAPPATLAGGSEIESEADPMLAAGGVQDIKGEY"
     gene            complement(8416..9579)
                     /locus_tag="VF226_00535"
     CDS             complement(8416..9579)
                     /locus_tag="VF226_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7072504.1"
                     /translation="MAFSFGAPWVTAVALKPIAAEADGLRAVPALAGALTWLGGGFGG
                     IAMGYVAERVGVRWTVTGGAVMIACGLALSSFGPGWPLLVGHGLFIGLLGLAGINAPL
                     YVYVSHWFDRHRGSALALISSGSYIAGAVWPPIFERAISTFGWRHSMIAYGLVEALVI
                     VPLAIMFLSQTPEKPEPAAPVSAGAARKRVLGWPPNLVFAMLMAAIFMCCVPMAMPQQ
                     HLVAFCSDVGITAARGALMLSVLLGTAFLSRQIWGLIADKVGGLRTVLVGSAAQLVTM
                     SAFLLTQDEYGLFTVSAAFGLGFSGLIPATVLTVRELFAAREAAWRIPMLLLCSGTGM
                     ATGSWLAGYIYDAFGFYAPAFAAGIAVNAAHLVLIGALVARRRATLAAAWSHA"
     gene            complement(10138..10359)
                     /locus_tag="VF226_00540"
     CDS             complement(10138..10359)
                     /locus_tag="VF226_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072505.1"
                     /translation="MLLQDFREALDPGFRIPNRGGTVSLDGHEGQTSDSQHEEAERNS
                     PEEVRLFNCTQQASRHGLLRAPQLGAGGH"
     gene            10468..>11586
                     /locus_tag="VF226_00545"
     CDS             10468..>11586
                     /locus_tag="VF226_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX7072506.1"
                     /translation="MRAFLLGSPQLGSAWILDDKGTTVLGTWDFPTPPVDASSRDYFK
                     AHKAGAQEPFLGRQEIGRIAGKPRFTISRAVRDADGQIHAIIVVAVYSDYFEALYRSA
                     ASWPGATAGLFLARGGQFDVLAQMSGVNATLGDLRNSLLELSQSQPSGVRILQLASGP
                     RVVGWSDSRDIPGMFAATSQSISAALREWIWRSAVTGGAAALSVLFFALLGVFGSRAE
                     ETERQLERQKLLMREVHHRVKNSLAIIAAITNAAARQCGSDTAAREQFKKLGSQIMSI
                     ATLYDLMQQAPGAGLVELNGMLHSLCLRLSESTGRQIEFLPDKPVMIDVDQAVSLMII
                     VNELVTNSIKHSTGPIKVVATGDPSEISIKVDNATGELP"
BASE COUNT         2216 a         3707 c         3544 g         2119 t
ORIGIN      
        1 accctgagca gatcgcgcag cgtgccgaac gtcttgcgct ggttcttgat gaccttgctc
       61 tcgacctcgc cgatgcccgt cacctcgacc gcgatctcgg ccatgcggcg tgcgttctcg
      121 ggcttgtaga gggaccacgc cagcgcggtc accgcctcgt cgatgcgcgc ctggccgccc
      181 tgctcgatct cgcgggcgta gatggccatg gccgcgcgcg cccgagcgat acaaatggga
      241 acgtgctctg caggtcccgg cgaaccgccg ccccccatgg tctttgtggg cgcatcctgc
      301 attgtcttcc tcgcctgaat tctagataat cgggatatgt tgtctcattc tggagatcag
      361 gacgaggctg gtcaactccc ggttggtccg ttacccctag gggagacagc acgaatgcgt
      421 gaagttctcg aatagtcgaa tttgcacgcc atggaacatc ggctaactgt gatgctcgat
      481 cgaaccgggt gcatccctgg ttgcacagtg aatagggagg ccagggtgag gcatgcgtga
      541 gtccggagaa cgcgggtcag cgctcaacaa ggctctcgcg gtgctcgagg ccattacggc
      601 ccagtcacag ccgatcggcc ttcccgacct gagcgcgcgg ctggcactgc cccggcagac
      661 cgttcaccgc attctcgttc agctcgagga agcgggcctc atcgttcgcg atccgcaacg
      721 ggatcgtttc tctgttggac cccgtctgtc gacgctcgcg ctcaatgcgc tgcagaccca
      781 taaccagtcg gccccgatcc gcgccgtcct gcagcagctc gtcgatgaca tcgaggagac
      841 ctgcaacgtc ggcgtcctcg atgggctgga cttcctctac cttgaacgca ttgagtgcca
      901 ctggtcgcta cgcgtgcatc tgcaggcagg cagcagggta cctgcccact gcacctctgg
      961 cggcaaggtg atgctcgccc acctggatga aacggtgcgt gcaaagctct tccgcaacat
     1021 ctcgctcaag gcatacacca gcacttcgat tacgcgcgtg tccgatctcg aagccgatct
     1081 cgcccaggtg cgggagcgcg gctatgccgt caataaccag gagttcacgg tcggcatcat
     1141 cggcgttgcc gtccccgtag ccgaccggca cggccgggtg ctggcagctc ttgcggtgca
     1201 tggtccggcg ccgcgcctgg acctggatag agccatcggc ttcgtgcctc ggctcaggga
     1261 ggctgcgact gtcatggcag aggcatggac aggaggcgat gatgccgcat ccacgaggtc
     1321 ccgtgccttc gcgcgggcga gcaagccggt ggcacccagc ttctagtctg cgattgacac
     1381 gccgagcact cgcgtgccac tgattgagac aagacgtctt gctttctgag acggcaagga
     1441 tctgccgctg tcagcggcgc gtttcgagga tacgctcatg acgaggatga ccccgagcga
     1501 agcgtttgtt gaaactctcg tcgcccatgg cgtcaccgac gtgttcggca tcgtcggctc
     1561 ggcgttcatg gacgctctcg atctcttccc gacggccggc attcgtttca tcccgacggt
     1621 tcacgagcag ggtgccggtc acatggcgga cggctacagc cgcgcttcgg gccgccacgg
     1681 cgtctgcatc gcgcagaacg gcccgggcgt cacgaacttc gttacctcca cggcagcggc
     1741 ctactgggcc cactcgccgg tcgtcgtgat cacgccagag acgggctcca acacgatcgg
     1801 gctcggcggc ttccaggaga ccgagcagct gccgatcttc tcaaagatca ccaagtatca
     1861 ggcccatgtc tcgcgccagg accgcatggc ggagctgacg gcgcgctgct tcgacatggc
     1921 gatgagcgag cgcggcccga cgcagctcaa tatcccgcgc gacatcttct atggcgaggc
     1981 cgactacgag atcccggtac cgaagaaaat cgcccgcggc cccggcgccg acgaggccct
     2041 ggacgaggcg gccgagctac tggcgagcgc caagttcccg gtgatggtcg cgggcggcgg
     2101 cgtcatcttc tccggcggga tagacgaggc caaggcgctg gcagaatacc tgcagatgcc
     2161 ggtggtcaac agctatctgc acaacgacag cttcccggcc gaccacccgc tgtggtgcgg
     2221 cccgctcggc tatcagggct ccaaggccgg catgaagatc atcgccgagg ccgacgtggt
     2281 tctggccctc ggcacccggc tcgggccgtt cggtaccctg ccgcagcacg gcctcgacta
     2341 ctggccgaag aatgcgaaga tcatccaggt ggacaccgac caccgcatgc tgggtctcgt
     2401 gaagcagatc tcggtgggca tcgtcggcga tgcgaaagcg gccgcccgcg cacttcatca
     2461 tcggcttgcg aaccgcgatc ccctcgcccg caagaacgct tccgagcggc tgcagaagat
     2521 caaggcggag aaggacgcct gggaggccga gctttcggag tggacgcacg agcgcgatcc
     2581 ctggagcctc gaggtctcga agaactcgcc gaacatgcat ccgcgccaga tgctgcgcga
     2641 gctcgagaag gccatgccga cgagcgccat ggtctcgact gacatcggca acatctgctc
     2701 ggtctcgaac agctatctgc gcttcaatca ggcgccgagc atgttcgcgg ccatgagctt
     2761 cggtaactgc ggctacgcgc tgccgaccat catcggcgcc aaggtcgccc ggccggaccg
     2821 cccggccatc gcctacgtcg gagacggcgc ctggggcatg agcttcggcg agatcctgac
     2881 ctgcgtgcgc gagagcatcc ccgtcactgc ggtggtcttc cacaacgagc agtggggcgc
     2941 ggagaagaag aaccaggtcg acttctacaa tagacgcttc gagggcgtga acctgaagaa
     3001 cccgagctgg gcggaggtcg cccgctcgct cggcgccgag gctgtcacgg tgcgcaatct
     3061 cggcgacgtc ggcgaagcgc tcgagaaggc cgtcgatgcg cagatgaagc acggcaagac
     3121 gactgtgctc gagatgatgg tgacccagga gctcggcgat ccattccgcc gcgatgcgct
     3181 gaagaaaccg aagcggcttc tcgacaagta caagcacacc aacgtcgatt gaaggcttcg
     3241 cccaggaacg acgttcgcac cccgacccta caggctgccg cgcggcagcc tgtttgtcgg
     3301 ttaatgcaca tggacttcaa ggtgcaggac gaaaaaggcc ggagcattcc tgccccggcc
     3361 agtggcactg tcagtggggg ctactgacca gtcccgaaat tgtaattcag tccgacccgg
     3421 aacacgtcga actccagcga tgccgggaag ctgtccgtga catgcgccgc cccggaactc
     3481 gttctgccaa cgaagtggta gtcttcctca ccgagatcca cgtgcagcca ttcggcacgc
     3541 accatccagc gatccgccag caggaactcg cctccggcgc cggccaccca gccgagatgg
     3601 ttctcgtctg cagagccttt cgccgtcacg acggtgaaag gaggtgtatt cgtgtcgacg
     3661 acggtcagat ctgagttggc cctgccccag gcgaaaccgc cggtgccgta aagcagcagc
     3721 cgcggggtca ccaagtaccc gaggcgaccg cgcaccgttc cgaagagctc cagctcgttg
     3781 tctatatccc aggctggcga ttcgttcagt gggtagggaa agagccgggc cctgtcgttg
     3841 aggtcggccc agctgacgtc ggcctcgaga ccccatacga acgccccatg ctggcggttc
     3901 cagccgatct gcagcccgcc gaggaagccg tcgtcgtcga ggcttttgcc cgaacgatcg
     3961 aacccgtcgc ctggcacagc ctcggaatag gccatgttgc catcccagcc gccggtgacc
     4021 cacccggcgt gcgcgccgaa gtaaagtccg gaccagctgg gcggggcgac caggatcggt
     4081 tcgtccttca tcgaaccata aggaatgccg ccaagatccg ccgcgttggc tgcacccgcg
     4141 cacgcaacca acgcaaccaa taacatacca aaccgcatca tttcccctcc aagttgcgag
     4201 acatctctat aacgcgagga cggcgtcaga gtctcggcga ctgggacaaa cttccgaggg
     4261 gcgttgctga gatgcgacac gggaggcagg ctgcaggagc cgtcagcggt cagcgtatcg
     4321 gaatgcaccg gctacggagc aaatctcgaa cggagcgatc actggaacga ccggcctgct
     4381 cgtctcaaag agggcgacgt ggtgaatcgg cggcggggcc tgcgaaattg gaccctgcga
     4441 ccgctcttga tcgaggccct ggaacgaaca tagaacacac aggtgcgcct gccgggctcc
     4501 tggcgcctgt gcggggttta ccagcgggtg gctccatgct aagggcctgt tccgcctcga
     4561 gggagacgtg gccgagtggc tgaaggcggc ggtttgctaa accgttatac gccctaaagg
     4621 cgtatcgggg gttcgaatcc ccccgtctcc gccaattgcc ccgtctgagt cttccttccg
     4681 aaccagcggc agccggaatt cgtcacgcat cacggcgtca atgccgtgaa ccgcgcctct
     4741 tgggttccga gtgcatctcg atgctcgcgg cagcatcagc tcctgcccgg tcgtcctgct
     4801 ggaacaggct gaccttcgcg cacgtcaaat tgcgtggttc ccgccagttt ctccagtcca
     4861 gtcctgcaac tgcagataag gtcggcacgc aaattgccaa ccacaatttt gattgaagtc
     4921 aatttactcc gtcgatttgt gaagccgagc accttttccg gcctcaatgc cgaccacctc
     4981 cgctgtggca tggcgtgccc ctatgaattg cacagaatgt cgtcaatact ggcccagcga
     5041 ctggccacac agggccccct cgacacggcc catgaagagc cggcgacgac acggaggaaa
     5101 agaaatggtt tcaaggtcga aagtccagct attcgcagcc gcggccgcgg cgactacgct
     5161 cgtgctcaca accctgctgc ctcagcaggc ggatgcgcag gaacgcaagt cgatccgctg
     5221 ggcgacatcc agcgtcgact cctatggcta caagatcgcc gcttcgatgg tgaagatcgc
     5281 cgaagaagcg ctcggcggcg aatacaccgt caccgtcaac ccgtatccgt ccacgacagg
     5341 cgccatgaag gccaccatgg atggcaacgg cgagatcagc tacaccgccg acgtcgggat
     5401 gacgcaactc tacaatcgcg aaggcggctt caagtcctac cagccgagca agggcatgct
     5461 cgtgcacacc tggtacgcct atccgatgga aaccttcatg atggtgctgg ccgataaggc
     5521 ctcgcagtac aagtgcttga aggacatcgc cggccagccg acgttcttca cgcccgccgg
     5581 ctacatgaac tggctgaact tccagcgcgt ctacaaggcg ctcggctatg acttcaagca
     5641 cgtccagatc gaccccaaga cacaggccga cgcgctgaag tccggcacca tcgcgggggc
     5701 agccgcttat accacggccg gccgctcgct gacgccgtac tggaaggaaa ccgaagtccg
     5761 catcgacgtg aaagccgtca atccttgtcc cgatgaaatc gagaaactga aggctgccgg
     5821 gctcagcatc agtccggtcg acccgagcgt cttcacgaag gatgtcggcg tcaaggaaat
     5881 ccagggcgtg ccgatcctgt tcggttacaa cgtgcgcgca gacatgcccg aagacgtcgt
     5941 ctacaaactg atcagcgcgt tctatgagaa ccgcgacgag ctggccaagg cagagcccgg
     6001 tttcacgcca atggcgaaag acttcatcgg catgcaagtc agcggcatca agtctgctcc
     6061 ggacgtgccg gtccatcccg gacttgcgaa gttcctgaag gaacacaacg cctgggatga
     6121 gaagtggaaa gtcgccgaga gcaagagctg atcggagacg cgctggaagt cgagacggag
     6181 cgggggcttg cgctcggggc aagctccccg cccttgctgt ctcgagtcct gcaatgatga
     6241 gagatccggc gacaccgttc acacgctgaa caactcggga cggccacaat aaaaaccagg
     6301 aaatgtcggg aggagcggcg gtgacagcta aagatgtcaa agaattcttt aatctgacca
     6361 acatcatttt gtttttcggg gccattcagc tcgcttggct cacctggtat ttttataccg
     6421 gttttggcgg gccgcaggag ctcgtcgcgc atgtgatgtc gatcacgctg atcctgcaaa
     6481 ttctgttcat gtaccgaaaa gattatttct acaaatggct gccgccacgc gtgaacgacc
     6541 tcatcatctt gttttatatc ggcatctgta tttacgcctt ttggcatttc tggtgggaat
     6601 tcgaagagat tgcgatttac cggcagggct cgtacacgac ccaggacttc atcgtcggcc
     6661 tgctggtgtt cctgctggtc atggaattgt cgcgactcgc ccatcccgtc ctgttctggg
     6721 tcaacgtcgt tctcattttc tatacactgt gggggtatct cagccccgtt gatttcttct
     6781 ggcatccggg cacgtcgttc tatcgcatcg tgacgtccag cacggtcgag ttttcgaccg
     6841 gcatttacgg catctatggt cagatcgccc tgaccctcat cgctgcattc ctgctgctgg
     6901 cggcggtcgc ccaaggcttc ggcgcccaga acgccatgat caacgtcatg cggcgccttg
     6961 caggcggatc gcgccagatg gtgccgcaaa cggcagtcct gggctcgctt tcgatcggca
     7021 tgatcagcgg cagcggctcg gccaatgccg tcgtcgtcgg cagcatcacc attccgctga
     7081 tgaagcgcta cggcctgccg ggcgcgtttg cgggcggcgt ggagtcggcc gcctccatgg
     7141 gcggtctcat catgccgccg atgatgggga tcggcgcctt cctgatgtcg caattccttg
     7201 gcgtccccta ttgggatgtc gttgtgcgcg gcttttccct cgccatcgtc tattacgtga
     7261 cgctcgcgct ggccgtctat ctggtctgcg tgcgtctgct gccctctgac aacgtgcctg
     7321 cgccgcaggt cccgctttac gatcgcgtta agacgtcaat cttctttctc tcggtcattt
     7381 tcctgattta cgagctgggc attgccggca ccggtgagct gttggccgca ctccacgccg
     7441 ccggcttcat gctcgcgctg ctgctcgccg ctttcctcta cttcaagtac gtgctgaagg
     7501 accccgcggt cgtcgaacag tcattgatcg gcaatctcaa gctgtccctc gaaacgcacg
     7561 cggacatgac gtcctatctg acgctgctgc tggcgacgct cggcatcatg atcggcctgt
     7621 tcaccgtcac cggcttcatc aaccgcatgg gcggcatgct gctcgacctc ggtgcatgga
     7681 acatcatggc catgattttc atggcctacc tctttggctg gctggtcggc gccggcctgc
     7741 cgccgacggc cacgtacatc atcggcgcgg tggtcattgt gcagccgctc gtcagcctcg
     7801 gcatcaatcc atgggtcgcg cacttcttcg ttttcctact gtcggtctgg ggtgaactat
     7861 ccccgccgac ctcgctgact gccgccatct cctcgcgcat cgcggaggcc tccttcatgc
     7921 gcaccatgtg ggaagcgctg aaactgtgcc tgccgatcac gctgatgacg ttcgccgtct
     7981 ttacccgttc ggaaatggtc gtcacgcccg gctggacgca ggtcctcaac accgctgcga
     8041 tgctgatcgg cacctgcggt atcgcattcg cgatattcgg gcgctacacc aggaacagcc
     8101 tggccgacgt cgctctgcgc ctcgtgctga cgctgcttgc gggcgccgtc ctgttccacc
     8161 ccaacgacac cgtcgcgttg agtctcagcc cactggtgct ggctggcgtg atcctcggca
     8221 tccttcggca ccggatcgtc gcgccaccag ctacgctggc cggggggagc gagatcgaaa
     8281 gcgaagccga tccgatgctc gccgcaggag gtgtccagga catcaaaggc gaatactgac
     8341 acggaacgta ccgcctgggc gggcggggcc cgtctctcag acttcacaaa agttacgcct
     8401 ttaacgatta ccgggctatg cgtggctcca tgcggcagca agggtcgccc ggcggcgggc
     8461 gacgagggcg ccgatcagca cgagatgcgc ggcgttcaca gcgatcccgg cggcaaacgc
     8521 cggtgcatag aagccgaagg catcgtagat gtagcccgcg agccagcttc ccgtcgccat
     8581 gcctgtgcct gagcacagga gcagcatggg gatgcgccag gccgcctcac gcgccgcaaa
     8641 caactcgcga acggtgagca cggtcgccgg aatcaagccg ctgaagccga gaccgaaggc
     8701 ggcggaaacc gtgaacagcc cgtattcatc ctgggtgagc aggaaagcgc tcatggtcac
     8761 aagctgcgcc gcggagccga cgagaacagt gcgcaggcca ccgaccttgt cggcgatcaa
     8821 tccccagatt tggcggctga ggaaggcggt gccgagcagc accgatagca tcagcgcgcc
     8881 gcgtgcggcg gtgatcccga catcgctgca gaaggcgacg agatgctgtt gcggcatcgc
     8941 catgggcacg caacacatga agatcgctgc catcagcatc gcaaaaacaa ggttcggcgg
     9001 ccagccaagg acccgcttgc gagccgcgcc cgcgcttacg ggggccgcgg gctccggctt
     9061 ttccggcgtt tgcgacagga acatgatggc aagcggcacg atgacgagcg cttcgacgag
     9121 cccgtaagca atcatgctgt gccgccatcc gaaggtgctg atggcccgtt cgaagatggg
     9181 cggccagacg gcgccggcga tgtaggagcc actggagatc agcgccagcg ccgagccgcg
     9241 gtggcgatcg aaccagtggc tcacatagac gtagagcggc gcgtttatgc cggcgaggcc
     9301 gagaagtccg atgaaaaggc cgtgccccac gaggagcggc cagcctgggc cgaaactcga
     9361 cagcgccagg ccgcatgcga tcatcaccgc gccaccggtc acggtccagc gcacgccgac
     9421 ccgttcggcg acatagccca tggcgatgcc gccgaaaccg cctccgagcc acgtgagcgc
     9481 gccagcgagc gctggtaccg cacgcaaacc gtccgcctcc gctgcgatcg gcttgagcgc
     9541 gacggctgtc acccacggcg ctccgaacga gaacgccatg atgctcaacg caatggcagc
     9601 aatcgtccac gatcggcgcg attcgataga agccgcgact ggaggagttt gcagcattgg
     9661 gtttgtcgcg agcctggtgg aaatccgaag gcggcgtaat ccccgaggcc tacaaatcga
     9721 gcccgcagca gcgcccgcga aggaaatcac gccgcctgaa agactagcat gcctgtgctg
     9781 gccaattgta cggcattcgg atcatgactg agctgcgcta tggcatggcc ctgtcgggga
     9841 gatgattggg gccgcggcct cttgcgtgct cgcctggcga gctggccctg agctgcccga
     9901 atcccgggcg tgctgccaac gagggctccg ccagcgaggt cgccgtcggc ctggcgctct
     9961 atgggctaac acggcggact aggacacaac cgccagctcg gttcggctcg gctgatgcga
    10021 attgtcctgg acctgcgatg atcggcctct tttcaaaagt gtcgcccggc atcaggttcc
    10081 caaaactcct gcgcaacgtc tcatcggcac aggctacagg gccccttgtc ttgcgcatca
    10141 atgaccgccg gctccaagct gcggtgcccg gagcagacca tgccgcgacg cctgctgagt
    10201 gcaattgaaa agccgcactt cttctggcga gttgcgctcg gcttcttcgt gctggctgtc
    10261 gctggtctgg ccttcatggc catccaggct gaccgtaccg ccacgattag ggatgcggaa
    10321 gccagggtcc aaagcctcgc ggaaatcctg gaggagcatg cagagcgcgc cctggccgag
    10381 ggcgagaagt tgttcgtggc tgtccggccc ctcgtcaagg catgggactt gaaggacccg
    10441 gtcctcgggg aaaggatcta cgaagagatg cgcgccttcc tgctgggaag cccccaactc
    10501 ggatccgcct ggattctgga cgacaaaggc accacggtgc tcgggacatg ggacttccca
    10561 accccgccag tggatgccag ctcccgagac tacttcaagg ctcacaaagc cggagctcag
    10621 gaaccatttc tcggccgaca ggaaatcggc aggattgctg gaaagccgag gtttaccata
    10681 agtcgcgcgg ttcgagacgc cgacggccag attcacgcga tcatcgtggt ggcggtctac
    10741 agcgactact tcgaagcgct ttaccgaagt gctgcgtcgt ggcccggcgc gaccgccggc
    10801 ctctttctgg ccaggggcgg gcaattcgac gttctggcgc aaatgtcggg tgtcaacgcc
    10861 acgttggggg acctgcgcaa ctcgctcctc gagctctcgc agtctcagcc gagcggcgtg
    10921 aggatactgc agctggcgag cggaccgcgc gtggtcggct ggtccgattc gcgcgatatt
    10981 cccggaatgt tcgcagcaac atcgcaatcc atttctgctg cgctgcgcga gtggatctgg
    11041 cgatcggcgg tcacgggcgg ggccgcggcg ctgagcgtgc tgtttttcgc cctcctcggc
    11101 gtgttcggct ccagagccga agaaaccgag cgccagctcg agcgccagaa gcttttgatg
    11161 cgagaggttc accatagagt gaagaacagc ctcgcgatca tcgccgccat caccaatgcc
    11221 gctgcccggc agtgcggaag cgataccgct gcacgcgagc agttcaagaa gctcggctcg
    11281 cagatcatgt ccatcgcgac gctttatgat ctgatgcagc aagccccagg cgccgggctg
    11341 gtcgagctca atggcatgct gcactcactc tgtttaaggc tgtccgagtc caccggcaga
    11401 caaatcgagt ttctgccgga caaaccggtt atgatcgacg tcgatcaagc cgtttcgctg
    11461 atgatcatcg tcaacgagct ggtgaccaac agtatcaagc actccacggg ccccatcaaa
    11521 gtcgttgcca ccggcgaccc gagcgagatt tcgatcaaag tcgataacgc aaccggcgag
    11581 ttgcca
//
LOCUS       DASVDU010000008         5259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_561129, whole genome shotgun sequence.
ACCESSION   DASVDU010000008 DASVDU010000000
VERSION     DASVDU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561129"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VF226_00550"
     CDS             <1..551
                     /locus_tag="VF226_00550"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7072507.1"
                     /translation="AGMGSKGIVASSAALGILLQQGIGDTIRYSLTPEPGGDRTLEVR
                     AAQELLQTMGLRTFVPTVAACPGCGRTTSTVFQELARDVQGMIRERMPEWREKYPGVE
                     SLNLAVMGCIVNGPGESKHADIGISLPGTGEQPAAPVFIDGKKAVTLRGPRIAEEFKA
                     IVEDYIEKRFGAGAREGRREAV"
     gene            complement(558..1538)
                     /gene="cysK"
                     /locus_tag="VF226_00555"
     CDS             complement(558..1538)
                     /gene="cysK"
                     /locus_tag="VF226_00555"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7072508.1"
                     /translation="MPQQTRPQPHFDPPGRGRIYDSIIETIGNTPLVRASRLSADEGI
                     KADLVLKLEFFNPLASVKDRIGVSMVLDLEKQGKIGPDTTLIEPTSGNTGIALAFVAA
                     ARGYRLILTMPESMSIERRKMLAFLGAELELTPRERGMKGAMARAEELLQQIPNSVMP
                     SQFTNPANPDIHRKTTAEEIWRDTNGEVDVIVSGVGTGGTFTGCAQVLKPRRPGLRMV
                     AVEPALSPVLSGGTHSPHQIQGIGAGFIPEVMDTSLIDEIVTVEDQQAIDMARKVARL
                     DGIAVGISSGAAVWAAVQIGKRPEMAGKRIVTIVPSFAERYLSTVLFEGL"
     gene            complement(1669..2529)
                     /locus_tag="VF226_00560"
     CDS             complement(1669..2529)
                     /locus_tag="VF226_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072509.1"
                     /translation="MKWIAVAIGAFYAFAGVVVLKRMAMDSMLDVVLEAITLEPTDPK
                     ERLKARVLTLGGCLTFASGLALLTLSPWANVFFAANAILQGGYLLWARKALPPESDEE
                     QQGRNQTLNAYVIYLAAFAFVVHAHSQGVFSHWPIDIGRFSWAIEPLAIGAAAAALWA
                     KWFLRTRLKNGPGSWGAGNEDGIEPGDHMPTPPLPERLRLAPEYGCWPTWDDVTGDNI
                     DPADLRLPDDLFIRLQRWDDAWQATFKPDDPASSGFGSQVADQLWRQEGEAIAGMLAQ
                     QWPGEVIVKL"
     gene            2699..4228
                     /locus_tag="VF226_00565"
     CDS             2699..4228
                     /locus_tag="VF226_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7072510.1"
                     /translation="MNLSLTLQTMARYVPDRPAITSDRGTLTYAAFERQAAAIAGALR
                     LRHGLEPGQRVGIVMENCAEFFPVLYGVWRAGLAAVPVNAKLHPKELAWILANSKARL
                     VVATPELADRLTAHTEESWPPIIATGSRDYAALLEGEPITRVPGRPEDEAWLFYTSGT
                     TGRPKGAVLTHRNLLFMVHTYTADIDFIGPEDTMVHAAPLSHGAGLYGLAHVARGSHN
                     VVLGGAFDPERVLATFADYRNVSLWAAPTMIVRLINHPKAGSADTRGLKTIIYGGAPM
                     YLADLKRAIELFGPRFFQVYGQGESPMTISGLSKAIHADAAHPDYEARLSSAGVARTG
                     VAFRIVDPDGRELPPGEIGEIITRSDCVMREYWNNPEATASALRDGWLWTGDLGSVDA
                     AGFLTLKDRSKDLIISGGTNIYPREVEEVLLTHPDVIECAVIGRPHAEWGEEVVAFVV
                     AREGGKATAETLDALCLDNIARFKRPKVYRFVEGLPKNNYGKVLKTDLRTWASKDGGA
                     R"
     gene            4384..>5259
                     /locus_tag="VF226_00570"
     CDS             4384..>5259
                     /locus_tag="VF226_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072511.1"
                     /translation="MANEKNAAVSRRGVLKGAATLAAASTVPTTWAIAKTRPLKIGLM
                     LPYSGTYAKLGENITFAVEMLIAEKGGKLGGREVEIVKLDDESKPELAPQNADRLVKR
                     DEVDVLIGTVHSGVQMGIHRVVRESGTLTIIPNAGNVAITRELCAPNVYRASFSNWQP
                     AYGMGVAAGKKGYKKGIWVSWDYAAGSESCDGFKQGLEENGGKFIEFLKLPFPQTNFQ
                     PLLAQIPGLDVDIVGAFFAGGGAVQFVKEYAAAGLKVPLCGNGFLTEGTLQAQGEAAE
                     GIETALHYGDGLDNPK"
BASE COUNT          936 a         1735 c         1702 g          886 t
ORIGIN      
        1 aggcgggtat gggctccaaa ggtatcgtcg cctcgtcggc ggccctgggg atcctgctgc
       61 agcagggcat cggcgatacc atccgctact cgctgacgcc cgagcccggc ggcgatcgca
      121 ccctggaggt ccgcgccgcg caggagctgc tgcagaccat ggggctgcgc acgttcgtgc
      181 cgacggtcgc tgcctgcccc ggctgcggcc gcacgacctc gacggtgttc caggagctgg
      241 cgcgcgacgt tcaggggatg atccgtgagc gcatgccgga gtggcgggag aagtaccccg
      301 gcgtcgaaag cctcaacctc gcggtcatgg gttgtatcgt gaacggcccg ggcgagtcga
      361 agcacgccga catcggcatc tcactgcccg gcaccgggga gcagcccgcc gcgccggtct
      421 tcatcgacgg caagaaggcc gtgacactcc gtggcccccg gatcgcggag gagttcaagg
      481 cgattgtcga ggattacatc gagaaacggt tcggggctgg cgcacgggag ggaaggcgcg
      541 aggccgtctg atccgcctca cagcccctcg aacagcaccg tcgagagata tcgctccgcg
      601 aaggacggca cgatggtgac gatccgcttt cccgccatct cgggccgctt gccaatctga
      661 accgcagccc agaccgcggc gcccgacgag atgccgacgg caatgccgtc gagtcgggca
      721 accttgcgcg ccatgtcgat ggcctgctgg tcctcgaccg tgacgatctc gtcgatgagc
      781 gacgtatcca tgacctcggg gatgaacccc gcgccgattc cctggatctg gtgcggactg
      841 tgcgtcccgc ccgacagcac cggactgagc gccggctcga cagccaccat ccgaagaccc
      901 ggccggcgcg gcttcagcac ctgggcgcag cccgtgaacg tgccgccggt tccgacgccg
      961 gaaacgatga cgtccacctc gccgttcgtg tcacgccaga tctcctcggc ggtggttttg
     1021 cgatggatgt cggggttggc ggggttcgtg aactggctcg gcatcaccga gttggggatt
     1081 tgctgaagca attcctcggc gcgggccatc gcgcccttca tgcccctctc gcgcggcgtc
     1141 agctccagct cggcgcccag gaaggcgagc attttccgcc gttcgatcga catggactcc
     1201 ggcatcgtca agatcagccg atagccccgc gccgctgcga cgaaggcgag ggcaatcccg
     1261 gtgttgccgg aggtcggctc gatcaaagtt gtatccggtc cgatctttcc ttgcttctcg
     1321 aggtcgagga ccatcgagac gccgatgcgg tccttcacgc ttgccagcgg gttgaagaac
     1381 tcgagcttca gcacgaggtc ggccttgatg ccctcatccg cgctgagccg agacgctcgc
     1441 accagcggcg tgttgccgat ggtctcgatg atgctgtcgt agatgcgccc gcggccaggc
     1501 gggtcgaagt gaggctgcgg tctcgtttgc tggggcatga tcggtcgctt gtctggaaag
     1561 tggctttgtc tggcatcaga ggtgtatcag ggagagcgct gctcgggttc caaggcacac
     1621 cgaattgcag cgtgatcggc ggacggctaa agaaccgttt tctcgtgcct aaagtttcac
     1681 gatgacttct cccggccact gctgcgccaa catgccagcg atcgcttcgc cctcctgtcg
     1741 ccagagctgg tcggccacct gactgccgaa ccccgaactc gccgggtcgt ccggcttgaa
     1801 cgtcgcctgc caggcatcgt cccatcgctg cagacgtatg aaaagatcat ccggcaggcg
     1861 gaggtcggcg gggtcgatgt tgtcgcccgt cacgtcgtcc catgtcggcc agcagccgta
     1921 ttccggcgcc aggcggaggc gctcaggcaa cggtggagtg ggcatatggt cccccggctc
     1981 gatgccatcc tcgttaccgg cgccccagga gcccggcccg tttttgagcc gcgttcgcag
     2041 aaaccatttc gcccagagtg cagcagcggc agcgccgatc gccaacggct cgatcgccca
     2101 cgagaagcgg ccaatgtcga tcggccaatg cgaaaagacc ccttgcgaat gcgcgtgcac
     2161 gacgaatgcg aacgcggcca gatagatcac atacgcattc agcgtctggt ttcgcccttg
     2221 ctgttcttcg tccgactcgg gtgggagggc ctttcgcgcc cacagcaggt agccgccctg
     2281 caggatggca ttcgcggcaa agaacacgtt tgcccacggg ctgagcgtca ggagggccag
     2341 cccgctggcg aaggtcaggc atcctccgag cgttagcacg cgcgctttca gccgctcttt
     2401 cggatcggtc ggctcgagcg ttatcgcctc cagcacgaca tcgagcatgc tgtccatcgc
     2461 catgcgcttg agcacaacga ccccggcgaa cgcgtagaat gcgccgatgg cgaccgcgat
     2521 ccacttcatt ccaccccccc ccccgcgaaa tacctcacac tagtctgatc gtcagccagc
     2581 gtgggagtgt tctgacaacc ggtgcaacgg gttatccgca acggacaatt gcgcgctggc
     2641 ccacgcccca ctagccttga gccggttgcc actcatgcgc ggagccgatg aactcgcgat
     2701 gaatttgtcg ctgacattgc aaacgatggc gcggtacgtg cccgaccgcc cggccatcac
     2761 ctccgatcgc ggtacgctga cctatgccgc ctttgaacgc caggccgcgg ccattgcggg
     2821 cgcgcttcgc ctgcgtcatg gcctcgagcc agggcaacgc gtcggcatcg tcatggagaa
     2881 ctgcgctgag ttctttccgg tgctctatgg cgtttggcgc gcggggcttg ccgcagtgcc
     2941 ggtcaatgcc aagctgcacc cgaaagagct cgcctggatc ctggccaaca gcaaggcccg
     3001 cctcgtcgtg gcgacgcccg agctggccga tcgcctgacc gcgcatacgg aagagtcctg
     3061 gccgccgatt atcgccaccg gctcgcgcga ctacgcggcc ctcctcgaag gagagccgat
     3121 cacccgtgtt ccgggccggc cggaggacga ggcatggctc ttctatacca gcggcaccac
     3181 cgggcggccc aagggcgcgg tgctgacgca tcgcaatctg ctgttcatgg tccacacgta
     3241 cacggccgat atcgacttca tcgggccgga ggacacgatg gtgcacgccg caccgctctc
     3301 ccacggggcc ggcctctacg gcctcgcgca cgtggcgcgc ggctcgcaca acgtcgtgct
     3361 cggcggcgcg ttcgacccgg agcgcgtgct cgccaccttc gccgactacc gaaatgtcag
     3421 cctgtgggcc gcgccgacca tgatcgtgcg gctcatcaat catcccaaag ccggcagcgc
     3481 cgatacccgc ggcctgaaga cgattatcta cggcggcgcg cccatgtacc tcgcggacct
     3541 gaagcgggcg atcgagctgt tcggaccgag gttcttccag gtctacggcc agggcgagag
     3601 cccgatgacg atctcaggac tgagcaaggc gatccatgcc gatgccgcgc atcccgatta
     3661 cgaggcgcgc ctgtccagcg ccggggtggc gcgcaccggc gtcgccttcc gcatcgtcga
     3721 cccggatggc cgcgaactgc cgcccggcga aatcggcgaa atcatcaccc gcagcgactg
     3781 cgtgatgcgc gagtactgga acaaccctga ggccaccgcg agcgcactgc gcgacggctg
     3841 gctgtggacc ggcgacctcg gcagcgtcga cgccgcggga ttcctgacgc tcaaggaccg
     3901 ctccaaggat ctcatcatct ccggcggcac gaacatctat ccgcgcgagg tcgaggaggt
     3961 gctgctgacg catcccgacg tgatcgaatg cgccgtcatc ggccgcccac atgccgagtg
     4021 gggcgaggag gtcgtcgcct tcgtcgttgc gcgggagggc ggcaaggcga cagcggagac
     4081 gctcgacgcg ctctgcctcg acaacatcgc ccggttcaaa cggccgaagg tgtaccggtt
     4141 cgtagaaggg ctgccgaaga acaactatgg caaggttctg aagacggatc tcaggacgtg
     4201 ggcatcgaaa gatgggggcg cccgctgagc cctgaccaga ccgaaaccgc tccttccaaa
     4261 taacttgcgc ttggctgctg gctgtgcgag cgagatgggc tggtgtccag cggatcaaag
     4321 caacaccaaa ccgtcatttc ccgccgcaac ggcaaatcct ggggaattca agggaggaaa
     4381 tccatggcca acgagaagaa tgccgctgtc tcgcgtcgcg gcgtgctcaa aggggccgct
     4441 acgctggcgg cggcatcgac cgtcccaacc acctgggcca tcgcaaagac acgccctttg
     4501 aagatcggac tcatgctgcc ctattcgggc acgtatgcga agctcggcga gaacatcacc
     4561 ttcgcggtcg agatgctgat cgcggagaaa ggcggaaagc tcggcggccg tgaagtggag
     4621 atcgtgaagc tcgacgacga gtcgaagccc gagctcgccc cgcagaacgc cgaccggctc
     4681 gtcaagcgcg acgaggtcga tgttctgatc ggcacggtcc attccggcgt gcagatgggt
     4741 attcatcgcg tcgtccggga aagcggcacg ctgacaatca tcccgaacgc aggcaacgtc
     4801 gccatcaccc gcgagctgtg cgcgcccaac gtctaccggg cctcgttctc caactggcag
     4861 cccgcctacg gcatgggtgt cgcggccgga aagaaggggt acaagaaggg catctgggtg
     4921 agttgggatt atgccgccgg ctcggaatcc tgtgatggct tcaagcaggg cctcgaggag
     4981 aacggcggca agttcatcga atttctcaag ctgcctttcc cgcagaccaa cttccagccg
     5041 ctgctggcgc agatcccggg gctcgatgtg gatatcgtcg gcgccttctt cgccggcggc
     5101 ggggccgtgc agttcgtaaa ggagtacgcg gctgcgggcc tcaaggtgcc gttgtgcggc
     5161 aacgggttcc tgaccgaggg cacgctgcag gcccagggcg aggccgccga aggcatcgag
     5221 accgcgctgc actatggcga cgggctcgac aacccgaag
//
LOCUS       DASVDU010000009        30423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1138549, whole genome shotgun sequence.
ACCESSION   DASVDU010000009 DASVDU010000000
VERSION     DASVDU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 30423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30423
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138549"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VF226_00575"
     CDS             complement(<1..341)
                     /locus_tag="VF226_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HEX7072512.1"
                     /translation="MRRKAEARRFGPALAVLMLPLALSLLLLATAAAAQKPLVTETKP
                     VAIAARPLAGFDREDLDRQDFGRLRWRGGLVLSSSAEEFGGWSGLVLDDGGRRLLAIS
                     DTGAWLTAELT"
     gene            complement(411..2291)
                     /gene="cobT"
                     /locus_tag="VF226_00580"
     CDS             complement(411..2291)
                     /gene="cobT"
                     /locus_tag="VF226_00580"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX7072513.1"
                     /translation="MAQAPKRKEPPAEPFKRVLGLAVRAIAGDDQLQVTYSAGRPELD
                     GHTVQLPEPSRVPSPREVAVIRGWADSLALTAAVHDKKIHRRLVPDAGPARAVFEAVE
                     RARIEALGANRMPGMAENLTAKIEDMYGHGRFADVKDRSEAPLEDALALLVRERLSGL
                     PPPKSAKSLVEVWRPWIEERAGKTLDRMDGLCEDQEAFGRLLRDVLRQLELTEELAEG
                     ESEENESEADENQDGGDEQTEASEDGSEGQEQVSEDQMAEGQEGESQDATDLSDADQF
                     DAEAEGDETAETREPWRPNASVLDDPEKFGYRVFTRAFDEEIAAEDLSTPDELERLRT
                     FLDKELRALSGAVSRLANRLQRRLMAQQNRAWEFDLEEGTLDPARLPRVIIDPTHPLS
                     FKRERDTNFRDTVVSLVLDNSGSMRGRPIMVAACCADILARTLERCGVKVEILGFTTR
                     AWKGGQSREQWLAAGKPPNPGRLNDLRHIIYKAADAPWRRAKRNLALMMREGLLKENI
                     DGEALAWAHNRLLGRPEQRRILMMISDGAPVDDSTLSVNSGSYLEQHLRQVIEEIENR
                     SPVELIAIGIGHDVTRYYRRAVTITDPSELAGAMTDKLVELFEEKTAAAPVRPVRRRV
                     LH"
     gene            complement(2330..2755)
                     /locus_tag="VF226_00585"
     CDS             complement(2330..2755)
                     /locus_tag="VF226_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7072514.1"
                     /translation="MRQSAVGGRQSVNGSKSSASVAIESYRDLRVWNEAMDLAEAACR
                     LTAQLPRSEIYGFTAQIRRAAASVPANIAEGYGRDSRGAYIQFLRVAQGSIKELETHL
                     MLAHRIDLLSREAIEPIVSKCDIVGRMLRGLIRSLERDA"
     gene            complement(2758..3750)
                     /gene="cobS"
                     /locus_tag="VF226_00590"
     CDS             complement(2758..3750)
                     /gene="cobS"
                     /locus_tag="VF226_00590"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEX7072515.1"
                     /translation="MHAQEQAGGPGLPDMKVSVRQVFGIDSDLEVPAYSNSNEHVPDL
                     DPDYLFNREVTLAILAGFKHNRRVMIQGYHGTGKSTHIEQVAARLNWPCVRINLDSHV
                     SRIDLVGRDAIVLKDGKQVTEFREGILPWALQSNTALVFDEYDAGRPDVMFVIQRVLE
                     VSGKLTLLDQSKVIRPHPAFRLFATTNTIGLGDTSGLYHGTQQINQGQMDRWSIVCTL
                     NYLPHEDEVRIVQQKVKAFSGTDAKRKVVSNMVRVADLTRNAFINGDLSTVMSPRTVI
                     TWAENAEIFGDIGFAFRLTFLNKCDELERPLVAEFYQRCFGEDLPESAANVALS"
     gene            complement(3834..4439)
                     /locus_tag="VF226_00595"
     CDS             complement(3834..4439)
                     /locus_tag="VF226_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7072516.1"
                     /translation="MIRIHRPRAEKRDAKQDWPCCQWKGCTQAGRYRAPMGRGRDGQY
                     YTFCAEHIRQYNSSYNYFDGMSNAEVEQYLQGVIYGHRPTWKVGVNSWSNGERQRISA
                     ADLHRFAEARAANAQGFQAWRARQGERQEYRRALKPLERKALQTLDLPETARGDEIKS
                     RYKALVKLHHPDANGGHRGSEDRLREIIQAYNYLKQAGLVQ"
     gene            4599..4877
                     /locus_tag="VF226_00600"
     CDS             4599..4877
                     /locus_tag="VF226_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX7072517.1"
                     /translation="MTNEQAIREKLMLALNPVRLDVINESELHAGHRSSPGTGQSHFR
                     VLIVSEAMRGKSRVERHRMVNEALAAELKGGVHALAIKAYAPGEDILP"
     gene            complement(4907..6226)
                     /locus_tag="VF226_00605"
     CDS             complement(4907..6226)
                     /locus_tag="VF226_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEX7072518.1"
                     /translation="MTTFQILLTASFVLILIIASAFFSGSETALTAASRARMHALEQE
                     GNRRAQLVNRLLAAPERIIGTVLLGNNLVNILASSLTTGLLIATFGEFGVLYATIVMT
                     AVIVIFAEVLPKTYAIAFPDRLALGVAPVMNVLIRVLWPLTALVQVIVSGILKLTPGR
                     ADDDANILAPHEELRGAIELQQKEGTVTRLDAAMLGGVLDLLDLKVLDVMVHRTKMQT
                     LNAAMPVDKLVEEVLKGHYTRLPVWEGEPENIIGVLHTKDLLVALGRANWDAEKIDIK
                     ELLKEPWFVPDTTGVKDQLNAFLKRKAHFALVVDEYGEVQGLVTLEDILEEIVGQISD
                     EHDVQDAAIRPQPDGTVNVDGSVPIRDLNRHMGWDLPDEEATTIAGLVIHEAQTIPEP
                     GQAFTFYGYRFEILRKNRNRITALRVKPLKASPPARRKVPASPTRLA"
     gene            complement(6308..7459)
                     /gene="aroB"
                     /locus_tag="VF226_00610"
     CDS             complement(6308..7459)
                     /gene="aroB"
                     /locus_tag="VF226_00610"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7072519.1"
                     /translation="MTIKSSQAAGAGEGHVARRVRVALGDRAYDVHIGPGLLNEAPRL
                     IGEALGGCRCGIVTDKNVAGAHLAPLEAGLSGAGRHAGSIVLPAGEATKSFAQLDRLC
                     QQLLTMGLERGDAVIALGGGVIGDLAGFAASIVRRGIRYVQVPTTLLAQVDSSVGGKT
                     GINTPQGKNLVGAFHQPSLVLADTSVLATLPARQMRAGYAEVAKYGLLGDATFFTWLE
                     ANWQSLFAYDPDALMHAIGRSVEMKAEIVARDETETGDRALLNLGHTFGHALEAWTGY
                     SDRLLHGEAVSIGMCLAIRLSEELRFCPPQTAARVAAHLKAVGLPTQIGDIPGDLPAP
                     QDVVRLMRQDKKVRQGRLTLVLINGIGEAFTTRDVEPAQIVDFLARVAG"
     gene            complement(7469..8098)
                     /locus_tag="VF226_00615"
     CDS             complement(7469..8098)
                     /locus_tag="VF226_00615"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX7072520.1"
                     /translation="MKHQSAAACERVRAIRASLGTRSLVMVGLMGCGKTSVGRRLSAR
                     LDLPFVDADEEIETAAGKTITEIFADHGEAHFRDGERKVIARLLKNGPQVLATGGGAF
                     MHPETRQNIKRAGISIWLKAELPVLMRRVMRRDNRPLLKTADPEARMRELMAERYPIY
                     AEADLVANSRDVPHDVIVDEILDALLKGPLAGETRGEPASAAAHEATGG"
     gene            8317..8460
                     /locus_tag="VF226_00620"
     CDS             8317..8460
                     /locus_tag="VF226_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7072521.1"
                     /translation="MPTLFRFLMITGIVAGIAYGGLWALAEYFEPVPHEVSTPVPGVK
                     VRQ"
     gene            8741..10330
                     /locus_tag="VF226_00625"
     CDS             8741..10330
                     /locus_tag="VF226_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072522.1"
                     /translation="MAPLLAPVERSELPPVMANDGSGLPFDPWAGIDLATTEQIMARL
                     ELPPLSPSLMGLWRRLMTETSSGPAGASMEQFTAVRAEALYRSGQLKELEALFSTAAP
                     ASADPLITALSARYDIAVGRRDRGCSGVREAAARKQDLPRQLGAEMLVLAGYCAAAAG
                     NRSAASLAADLAREEGLDDAGLISALNAIASGGKPRPQRSGPITPTHYRLLQLAKAVD
                     PKTIVERADAALLSALVLDPTTDDRLRVAAAEAAAQRNVITPDALAEVYRSQRFSPDD
                     LARPLASDVDPLLRRAVLFQAAESERDPVRQAQIIRAFVDDARQAGVYVHALMSVAPL
                     AEAIPQSAELAWFGETAIEAALVSGNHGSARAWWTALAAGGRDGDRLEHWLALSDIAN
                     ADLRAPRGLHLPAVEALALRGGFSAPVLHRLATVLDALDYQVPMQLWHVASSTPQPND
                     GYLPETGVLTELKEASSREQFARTVLLVMQTLGPKGAEGAHIIALGDAIRALKRIGLE
                     AEARRVGLEALFGAWPRSAIH"
     gene            10341..11288
                     /gene="xerD"
                     /locus_tag="VF226_00630"
     CDS             10341..11288
                     /gene="xerD"
                     /locus_tag="VF226_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX7072523.1"
                     /translation="MPNSDKLIDSFLEMLAAERGAAANTLEAYRRDLENFSAFLGKRH
                     VELAAAGPADISAYLRFLTEQGMAASSRARRLSALRQLYKFLLSEGRVAEDPVLGIQG
                     PKVNQALPKTLSVAEVDRLIATARARIEGTTGRERFVALRLYCLLEMLYATGMRVSEL
                     VSLPRSVLHGDPRVLTIKGKGGRERLVPLNAAARAALDRYLEVGQHPEDGLAPLARTR
                     WLFPSRSAEGHLTRQRLAQELKELAEAAGLDPRRVSPHVLRHAFASHLLDRGADLRAV
                     QQLLGHADISTTQIYTHVLEERLRRLVLEHHPLAGSKLR"
     gene            11463..12431
                     /locus_tag="VF226_00635"
     CDS             11463..12431
                     /locus_tag="VF226_00635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX7072524.1"
                     /translation="MDRQPIRTYIEFEKPIAELESKVAELKALSSGDNAVSLSEEISK
                     LEQKAQQILAETYAKLTPWQKTQVARHPERPHTLDFVNALVEEFTPLAGDRYFAEDQA
                     IVGGLGRFRGRSVMVIGHEKGSDTESRIRHNFGMARPEGYRKAVRLMDMADRFGLPVI
                     TFIDTAGAYPGIGAEERGQAEAIARSTDRCLSLGVPIVAVVIGEGGSGGAVAIATANR
                     ILMLEHAIYTVASPEAAASILWRDSARAIDAATNMKITAQDLMQLKVIDAIIPEPVGG
                     AHREPEKAIAATGEAIAKALAEFEGQSPAEIRSQRHERFMQIGRTL"
     gene            12637..13635
                     /locus_tag="VF226_00640"
     CDS             12637..13635
                     /locus_tag="VF226_00640"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEX7072525.1"
                     /translation="MRGIYVGILITAAALLAACAKIELAPHLKPLSKETMMLLGRKDM
                     RIDQPIFVRIFKEESELEIWKQRDDGRFYHFKTYPICNWSGDLGPKMQQGDRQAPEGF
                     YRVTRHQMNPNSNFHLAFNLGYPNAFDRAHNRTGEFLMVHGKCKSAGCYAMTDEPMEE
                     IYGLAREAFINGQEAFEVHAFPFRMTDEKMALHKNSPHYEFWKTLKEAYDYFELTRVP
                     PTIAVCEKRYVVNAKFSGSIDPAKLDPVGRCPPYQKIEPEPFVPTGIDQQIAEERIVA
                     PGPKKRTLASIRNGSLSLTPVTASVMPADPAPTGTSTTGVFVNGWSSVSRALGLAK"
     gene            13754..14554
                     /locus_tag="VF226_00645"
     CDS             13754..14554
                     /locus_tag="VF226_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7072526.1"
                     /translation="MRAKINGIEMTYEVSGRTDAPAVVLHHPLATNLSTWDALTAALE
                     PTYRVIRMDARGHGKTEAPDGAYSFETLTADVIGLLDHLDLAKAHFLGLSMGGMVGQY
                     LGILHPDRFHSLCLVSTTSAVPSQAWSLWDERIRVAATGDLSSLVDAAVARWVTPATA
                     KNKPELVAQLKEMMLSTPAKGYGGWCHAIRNLNITARLKEIELPTRVIVGQADPATPV
                     LAAKVIHEEIAGSDLVVIPGVSHMLHVEAPEAFHPHVLAFLAAHSRSV"
     gene            complement(14620..15285)
                     /gene="rplS"
                     /locus_tag="VF226_00650"
     CDS             complement(14620..15285)
                     /gene="rplS"
                     /locus_tag="VF226_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7072527.1"
                     /translation="MNIIQVLESEQMSAVTSKRVVPEFGPGDTVRVMVKVIEGDNVRT
                     QAYEGVVIARSGSGLNENFTVRKISYGEGVERVFPVYSPYIADIEVLRRGKVRRAKLY
                     YLRGRRGKAARIVERTDARARRLNAAFKGFKRPKGQPDDLKQISGITPELEAQLKKYN
                     VLRFDQIAAFSDEDIANLDELLKLNGQIERDDWVGQANRLVAEAAAAEVPAEEGEESQ
                     QSA"
     gene            complement(15433..16167)
                     /gene="trmD"
                     /locus_tag="VF226_00655"
     CDS             complement(15433..16167)
                     /gene="trmD"
                     /locus_tag="VF226_00655"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7072528.1"
                     /translation="MSAPTPHDATGMAPWRATVLTLFPEMFPGPLGYSLSGQALDSGV
                     WSLATYNIRDFGIGRHRTVDDNPAGGGPGMVMRCDVAAAAIDAARKQQPDAPLIYMTP
                     RGRPLTQRLVRELAAGPGVMVLAGRFEGVDQRVLDARGAREISIGDYVLSGGELAAMV
                     LIDACVRLLPGVVGAHESLSEESFEDNLLEYPHYTRPREWEGRSIPEVLLSGDHKRIA
                     EWRQEQRLRITRERRPDLIAKAGEKS"
     gene            complement(16283..16822)
                     /locus_tag="VF226_00660"
     CDS             complement(16283..16822)
                     /locus_tag="VF226_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072529.1"
                     /translation="MSLLYDFARDYQAALVGFGQVFAASALLVGIFYWLKMRLMKKLG
                     WQSRSRPKRQSARRVRSKAAGRISSKAAAKATRPSKAAPQEFLAPSASQWVPPRISPE
                     AHSQRAAALIHEVVARGERARELHQRSLVRLEAADYAFQRLLLEVAEVINLPQAQEVG
                     DSPRPAELILPEQVRPLAA"
     gene            complement(16999..17877)
                     /gene="gluQRS"
                     /locus_tag="VF226_00665"
     CDS             complement(16999..17877)
                     /gene="gluQRS"
                     /locus_tag="VF226_00665"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX7072530.1"
                     /translation="MTRPVLRFAPSPNGELHLGHALSALTGFEMARHLGGRFLLRIED
                     IDVNRTRPDYVQAIFEDLAWLGLEWEEPVLHQSQHFDDYRVAAEVLKRTGLLYPCYLT
                     RAELRAFAIEHPELVDPDGAPRITRALLPLPPEEQIRRRAAGQPFAMRLDMARALEVL
                     KERTGSSELSFAELDADGRAIRRIRARPERWGDVIIVRKDIPASYHLAVVVDDARQGV
                     THVTRGLDLFAATDVHRLLQVLLDLPEPVYHHHRLIADATGRKLSKSARDTSLKALRA
                     AGLTPHDIRRLIGLSS"
     gene            17982..18668
                     /locus_tag="VF226_00670"
     CDS             17982..18668
                     /locus_tag="VF226_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX7072531.1"
                     /translation="MAKLRNSSSRADVDVGIIETAEDIRAGMRAIRRKCPHLRRVHDV
                     TGDPPLRRRPAGFEGLARIIVGQQLSTASAAAIWSRLELAVAPLAPETLLAASDEVLR
                     SAGLSTPKLRTLRNVARAAAEGELAIETLTALPDEAIHEQLTRISGIGPWTADIFIMF
                     CLGRRDAFAAGDLALQVAAEGAMGLDSRPSAAELLAIAERWRPWRGVAARLLWAYHPI
                     LKKRGSGAPL"
     gene            18678..19334
                     /locus_tag="VF226_00675"
     CDS             18678..19334
                     /locus_tag="VF226_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7072532.1"
                     /translation="MRHLDGPRLEPSRPVTGLVVLLHGYGANGEDLIGLAEAWQPLLP
                     QIAFVAPNAPEPLPGAGFSARQWFELTFRDLHELWRGVNAAGPILEKFLDSELGRYGL
                     PPNRLALVGFSQGTMMALHVGLRRAAAPAAIIGYSGVIAGPEHLEAQLKARPPVQLIH
                     GELDDVIPIEALHITREALARVRIPVEWHIRPGLGHGIEPVGLKLGGSFLAQALKPGH
                     "
     gene            19529..20059
                     /locus_tag="VF226_00680"
     CDS             19529..20059
                     /locus_tag="VF226_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7072533.1"
                     /translation="MPALVLNADFRPLSYYPLSLWSWQDTIKAVFLNRVNIVSEYEKT
                     VRSPSFEMRIPSVVALKNYVRPALYPAFTRFNVFLRDRFTCQYCGSKEDLTFDHVIPR
                     SRGGQTRWDNVTTACAPCNLAKGGAMPDKAGMRPKQPPYRPTVFELHRNGRLFPPNYL
                     HESWLDYLYWDTELEP"
     gene            complement(20088..21635)
                     /locus_tag="VF226_00685"
     CDS             complement(20088..21635)
                     /locus_tag="VF226_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7072534.1"
                     /translation="MSSTEDLFARYGPAYRWLATCSVMISAIAVVLSTTIVNVAIPDV
                     MGAFGISQVEAQWLSTGFLAAMTATMLLTDWADKAFGQRLSMIGALLTFMAGSVLGGM
                     APNEQVLTLARVIQGAAAGVVQPLGMVLLFQVFPPDQRGSAMGIYGIGVVLAPALGPW
                     IGGLLIDTFNWRYVFYLGVPFAILGVVLSNFFLPTRQQSGPRPGFDWIGMGLLGLFLS
                     MLLTALTNGQRQGWNSDPILLQFAISVVAITSFAWWELRAEKPMLDLRVFLEVPFAAA
                     ALVSFIMGAGLFGSTYLVPLFVQTIQGFTPTEAGLLLMPSGFVLVLVFPLAGRLADRL
                     PIGLLIGVGMAIFAYSSWLTASIEINSAFWTVAWWTVISRIGLGLVFPALTAGSLRVL
                     PRELIAQGSGAINFVRQLGGAFGVNLLSVFLERRTIFHADALAATQTSDNSTTVALLH
                     QVAALLGHAGLPDIQQIPAALWYLGQMIAIQANTLAFRDGFLVVMVIFLVALVPTWIL
                     HRAIGRR"
     gene            complement(21622..22791)
                     /locus_tag="VF226_00690"
     CDS             complement(21622..22791)
                     /locus_tag="VF226_00690"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX7072535.1"
                     /translation="MSAVTDLRTKEAPSIAEPKAEKAFSAPLPFWQRRRFRMSAIGVI
                     ALLFLAVAADWLVHRWTHVYIDDSRIAANLITVSSEVNGRVLEVPVVIGDAVKKGDLL
                     VRIDRSQTKLERDAIEAQIAAIEAEQKQLRAQQDMIRVQIAKKVDAAKAEVAAAEAAY
                     RASRAVLQKAHSHYDRIEALAQRKVVAQENLEEALAERTTAEQQQNATEARIATAKAN
                     LAVVEAQAAEIEVLERQIAVLESRKSAQIAERDRKDVEIDRGTIRAAFDGVVGATFVD
                     PGEYVSPGARLLVYHDPSNVWVDANVKETDFSRLKLGAPATITVDAYSDLEFKGKVIR
                     LGEAATSQFALLPSPNPSGNFTKITQRVPIRVSVEQKDGLLRPGMMVEVSIDVVD"
     gene            complement(22788..23219)
                     /locus_tag="VF226_00695"
     CDS             complement(22788..23219)
                     /locus_tag="VF226_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7072536.1"
                     /translation="MSTAQEFALELSRVSRRWRARLDERLRHLDLTQARWVALLYLSR
                     SGPISQRELADMIGIEGPTLVRLLDGLEQQGLVERRACGSDRRVKKVALTERAGPTLA
                     EINRIAGELRGELLAGIPAGDLAIAGRVLKTIADRLEGAGI"
     gene            complement(23389..23598)
                     /locus_tag="VF226_00700"
     CDS             complement(23389..23598)
                     /locus_tag="VF226_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072537.1"
                     /translation="MIRNNINPLQWHSAIGVARQICARVFRDGGTPADAVTAFGLEHV
                     EGLSWSKAVDVIAERLCLRNFRMAA"
     gene            complement(24597..25121)
                     /locus_tag="VF226_00705"
     CDS             complement(24597..25121)
                     /locus_tag="VF226_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX7072538.1"
                     /translation="MPSPLPVSERDPAYRWGAAALLAAVAIILTALGFEHIGGYRPCA
                     LCLQERYAYYIGIPLLFGALVLVSTGQRRLAAALFALVAIGFLVNAGLGVYHAGVEWK
                     FWAGPETCQFALEPLGAGPGGLLKRLESVRVVRCDEAAWRFLGLSFAGWNAVLSLLLS
                     LSIFTAARLSWQRA"
     gene            complement(25125..25706)
                     /locus_tag="VF226_00710"
     CDS             complement(25125..25706)
                     /locus_tag="VF226_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX7072539.1"
                     /translation="MLRKLYDWTMDLASHRRAPHALFWVSFIESSFFPIPPDVLLMPM
                     VLAKRMRAWAYATIATVGSVLGGAAGYLIGLFLFDLLGKPLLDLYGYAEQFERFASTY
                     NEYGALIVFFFGVTPFPYKVITIASGVTQLNFVIFVLASIASRGLRFYLVSGLLYWFG
                     APIREFIEQRLGLVTTVFFVLLFGGFITLRYLV"
     gene            complement(25775..26962)
                     /gene="hemW"
                     /locus_tag="VF226_00715"
     CDS             complement(25775..26962)
                     /gene="hemW"
                     /locus_tag="VF226_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7072540.1"
                     /translation="MAGNPDEAGFGVYIHWPFCASKCPYCDFNSHVRASGVDEPRFLA
                     AFRRELQTTRERIGRRTVNSIFIGGGTPSLMQPSTVAGILDEIGRLWSIDSHVEVTLE
                     ANPSSVEAGRFRGYRAAGVNRVSLGVQALRDVDLRQLGRLHTVAEAKAALSVASSTFA
                     RFSFDLIYARPGQTLEAWRAELEEAIIIAGGHMSLYQLTIEDGTPFAALHAAGKLRVP
                     DGEQASALYELTNEIMSARGLPAYEISNHAVPGEESRHNLLYWRYGEYAGIGPGAHGR
                     LIVGRERLATMDERAPERWLEKVEQFGHGLIAAEPLSRGEQADEALLMGLRLAEGLDL
                     ERLARIGGVRPREGTVAELCRQGLIAVVDGGRRIKATPSGRMVLNELVLRLSAAFDDP
                     LPV"
     gene            complement(26946..27569)
                     /gene="rdgB"
                     /locus_tag="VF226_00720"
     CDS             complement(26946..27569)
                     /gene="rdgB"
                     /locus_tag="VF226_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7072541.1"
                     /translation="MRKLQKGTRLVVASHNPGKLREIADLVGPYGLEVVSAKDLNLPE
                     PEETETTFAGNARLKAVAAATRSGLPALADDSGLEVEALGGRPGIYSARWAGPAKDFS
                     LAMQRVADELTAVGAWSGAGPRANFTCALSLAWPDGEAQIFEGKVFGRLVWPPRGSRG
                     FGYDPMFLPDGETLTFGEMDPARKHAISHRARAFALFVDACLDGRQS"
     gene            complement(27569..27979)
                     /locus_tag="VF226_00725"
     CDS             complement(27569..27979)
                     /locus_tag="VF226_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7072542.1"
                     /translation="MTPARILETVLYVSDLEAAERFYRDVLGMEPFSKARGRQLFYRC
                     GNQVLLLFNPQATSKPPGPNALPVPPHGAVGPGHVCFGASADEIESWRQRLIDRGVPI
                     EADFEWPGGGRSIYFRDPAGNCLEFAEPRIWGLE"
     gene            complement(28016..28729)
                     /gene="rph"
                     /locus_tag="VF226_00730"
     CDS             complement(28016..28729)
                     /gene="rph"
                     /locus_tag="VF226_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7072543.1"
                     /translation="MRPSKRKPDELRRVSFERAVSMHAEGSCLIKFGHTHVLCTASLE
                     ERVPQWLKNQGRGWVTAEYGMLPRATSERTRREATIGHATGRTHEIQRLIGRALRSVV
                     DLQKLGERQISIDCDVLQADGGTRTAAITGAWVALHDCIQWMRARDMVGGDVLKDQVA
                     AVSAGIYEGQPVLDLDYAEDSEADADANFVMTGSGGIVEVQGTAEKEPFSQEQFDELM
                     VLARKGIKELVGLQKLTVA"
     gene            28910..29968
                     /gene="hrcA"
                     /locus_tag="VF226_00735"
     CDS             28910..29968
                     /gene="hrcA"
                     /locus_tag="VF226_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7072544.1"
                     /translation="MTGTISELNKLNERSRAIFRQIVENYLETGEPVGSRNISRNLPM
                     TLSPASVRNVMSDLEQLGLIYAPHTSAGRLPTQMGLRLFVDGLLELGDLSEEERKHIQ
                     SQIAFRRDKSIEQLLEEAGEMISGLSHCAGVVLAEKQVSRLKHIEFVPLEPGRALVVL
                     VGDDQTVENRIVSVPAGLPPSALVEAANYLNAHVRGLSIDEARSRIEQQIASARAELN
                     TLTQKVIKAGLAEWSGSLGDRQSLIVRGQSNLLKDLTAIEELERIRQLFEDLETKRDL
                     IQLLGLAERGEGVRIFIGSENKLFSLSGSSLIVAPFQDKSRKIVGVLGVIGPTRINYA
                     RIIPMVDYTAKLVGRLLT"
     gene            30067..>30423
                     /gene="grpE"
                     /locus_tag="VF226_00740"
     CDS             30067..>30423
                     /gene="grpE"
                     /locus_tag="VF226_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7072545.1"
                     /translation="MSAKPSPEEQPAPHEASAEAQKSEAAAAEDNRPQAGSNEQGPAT
                     AEAGSERPAKQEAAAAEAEAGQSAGAEARVAELEAQVADLTDRLLRAHAEIDNIRKRA
                     ERERLDTAKYAITKFAG"
BASE COUNT         5552 a        10016 c         9585 g         5270 t
ORIGIN      
        1 taggtcagct ccgccgtgag ccatgcgccg gtatcggaga tggccagaag acggcgtccg
       61 ccgtcatcga gcaccaatcc cgaccagccg ccgaattcct ccgcagatga gctgagcacg
      121 aggccgccgc gccatcgcaa ccggccaaag tcctgccgat ctaggtcttc tctgtcgaac
      181 cccgccaagg gcctggccgc gatcgcgaca ggctttgtct ctgtgacaag cggcttctgg
      241 gccgccgcgg ccgtcgcgag aaggagcagc gacagggcga gcggcagcat cagcaccgca
      301 agggcggggc caaaccggcg cgcttccgcc ttccgacgca ataccggctc ctcctgttaa
      361 gtgctgctcg gattatgcag ggccggagtt aaatttctag tgcttcagcc ttaatgcaaa
      421 acgcgccgac gcaccgggcg cactggcgcc gctgcggttt tctcctcgaa gagctcgacg
      481 agcttgtccg tcatcgcccc ggccagctcg gacgggtccg taatggtcac ggcgcggcgg
      541 taataccgcg tcacgtcgtg tccgatgccg atggcaataa gctccactgg cgagcggttc
      601 tcgatttcct caatcacttg cctgaggtgc tgctccagat aggagcccga attgaccgac
      661 agcgtcgagt cgtcgaccgg agcgccgtcc gagatcatca tcaggatgcg gcgctgctca
      721 ggcctgccca aaaggcgatt gtgcgcccag gcgagcgcct caccgtcgat gttctccttc
      781 aacaggcctt cgcgcatcat cagtgccaga ttgcgctttg cccgccgcca gggcgcatcc
      841 gcggccttgt agatgatgtg acgcagatca ttgagccggc caggatttgg cggcttgccg
      901 gcggcgagcc actgctcgcg cgactgcccg cctttccacg ctcgcgtggt gaagcccaga
      961 atctccacct tgaccccgca gcgttccagc gtgcgcgcca gaatatccgc acagcaggcc
     1021 gcgaccatga tcggccgtcc gcgcatgctg cccgagttgt cgagcaccag gctcaccacc
     1081 gtatcgcgga aattggtgtc gcgttcgcgc ttgaaggaaa gcggatgcgt cggatcaatg
     1141 atcacgcgcg gcaagcgggc cggatcgagc gtgccttcct ccaggtcgaa ctcccaggcg
     1201 cggttctgct gcgccatgag ccgtcgctgg aggcgattgg ccaatcgtga gacggcgcca
     1261 gagagggcgc gcagctcctt gtcaaggaat gtcctgagcc gttcgagctc gtcgggcgtg
     1321 gacagatcct cggccgcaat ctcctcgtcg aaggcgcggg tgaacacgcg atagccgaac
     1381 ttttctgggt cgtcgagaac ggatgcattc ggccgccagg gctcgcgggt ttcggctgtt
     1441 tcgtcgcctt cggcttcagc atcgaactgg tcagcatcgg aaagatcggt cgcgtcctgc
     1501 gactcgcctt cctggccctc ggccatttgg tcctcggaga cctgctcctg cccctccgag
     1561 ccgtcctcgc tcgcttcggt ctgctcgtcc ccgccgtcct ggttctcgtc cgcctccgac
     1621 tcgttctcct cggattcacc ctcggccagc tcctcggtga gctcgagctg gcgaaggacg
     1681 tcgcgcagca gacggccgaa tgcctcctgg tcctcgcaaa ggccgtccat gcggtcgagc
     1741 gtcttgccgg cgcgctcctc gatccatggc cgccagacct cgacgaggga cttcgcagac
     1801 ttcggcggcg gcagaccgga caagcgctcg cgcaccagca acgccagcgc atcctcgagc
     1861 ggcgcctcgg aacgatcctt cacgtcggcg aagcggccgt gaccgtacat gtcctcgatc
     1921 ttggccgtca gattttcggc catgccgggc atccggttgg cgccgagcgc ctcgatgcgc
     1981 gcccgctcca cggcctcgaa aacggcgcgc gcagggccgg catccggaac gaggcggcga
     2041 tggattttct tgtcgtgaac ggcagcagtg agcgccaggc tgtccgccca gccgcggatg
     2101 actgccacct cgcgcggcga aggcacacgc gagggctctg ggagctgcac ggtatggcca
     2161 tcaagctcgg gccggccagc agaataggtg acctgtagct ggtcgtcgcc cgcgatggcg
     2221 cgcaccgcca gaccgagcac gcgcttgaac ggctcagccg gtggttcctt gcgctttgga
     2281 gcttgggcca tcgttactcg gcagttggca gtcggttgtc ggcagtcgat caggcgtcac
     2341 gttccagcga tcggatcagg cctcgcagca tgcggccaac gatgtcgcac ttggacacga
     2401 tcggctcgat cgcttcccgc gagagcaagt ctattctatg cgcgagcatc agatgggttt
     2461 ccagctcctt gatggagccc tgcgcaaccc gcaagaactg gatataggct ccgcggctgt
     2521 cgcgcccata tccttcagca atattggctg gcacagaagc agcagcccgg cgaatttggg
     2581 cagtgaagcc gtagatttcg cttctcggca gttgcgcagt caaccggcaa gccgcctcgg
     2641 caaggtccat agcctcattc cagacgcgca agtcacggta cgattctatc gcgaccgacg
     2701 cgctcgactt ggagccattt accgactgcc gaccaccgac tgccgattgc cgcatcatca
     2761 gctcagcgcc acattggcgg cgctctccgg caggtcctcg ccgaagcagc gctgatagaa
     2821 ctcggccacc agcggtcgct cgagctcgtc gcacttgttg aggaacgtca accggaaagc
     2881 aaagccgatg tcgccgaaga tctcggcgtt ctcggcccac gtgatcaccg tgcgcgggct
     2941 catgaccgtc gaaaggtcgc cgttgatgaa cgcattccgg gtcagatcgg ccacgcgcac
     3001 catgttcgag acgaccttgc gcttcgcatc cgtgccggaa aacgccttca ctttctgctg
     3061 cacgatgcga acctcgtcct catgcggcag atagttgagg gtgcacacga tcgaccagcg
     3121 gtccatctgg ccctggttga tctgctgcgt gccgtgataa aggccggagg tgtcgcccag
     3181 accgatcgtg ttcgtggtcg cgaacaggcg gaacgccgga tgcggtcgga tcaccttcga
     3241 ctggtcgagc agcgtcagct tgcccgaaac ctcgagcacc cgctggatca cgaacatcac
     3301 gtcgggccgg ccggcatcat actcgtcgaa caccagcgcc gtattggact gcaacgccca
     3361 gggcaggatc ccttcccgga actccgtcac ctgcttgcca tccttgagga cgatggcgtc
     3421 gcggccgacg agatcgatac ggctgacgtg gctgtcgaga ttgatgcgca cgcacggcca
     3481 attgagccgg gccgcgacct gctcgatgtg cgtcgatttg ccggtgccgt gatagccctg
     3541 gatcataacg cgccggttgt gcttgaagcc agcgaggatg gccagcgtca cctctctatt
     3601 gaagaggtag tccgggtcca ggtccggcac atgctcgttc gaattggagt aagccggaac
     3661 ttcgagatcg ctgtcgatgc cgaagacctg gcgcacggac actttcatgt ccggaaggcc
     3721 ggggcccccg gcttgttctt gcgcatgcat gctgatggtt tttccgataa gttccgttga
     3781 cactactcga cacgaccggc gccgagtccc gctgggcgac ctcgaaggcc gccttattgc
     3841 acgagccctg cctgcttcag atagttataa gcttggatga tctcacgcaa cctgtcctcc
     3901 gacccccgat gaccgccgtt ggcatcgggg tggtgtagct tgaccagcgc cttgtagcgc
     3961 gacttgatct cgtcgccccg cgccgtctcc ggcaaatcaa gcgtttgcag ggccttgcgc
     4021 tcgagcggct tcaacgcacg ccggtattcc tggcgctcgc cctgccgtgc ccgccacgct
     4081 tgaaatcctt gtgcgtttgc cgcgcgcgct tccgcgaacc ggtgcaggtc ggcggcggaa
     4141 atgcgctgcc gttcgccgtt cgaccatgaa ttcaccccga ccttccacgt cggccggtga
     4201 ccgtagatga caccctgcag atactgctcg acctccgcat tgctcatccc atcgaagtag
     4261 ttgtacgagc tgttgtactg ccggatgtgc tctgcgcaga aagtgtagta ctggccatcc
     4321 cggccgcgcc ccatcggtgc ccggtagcgg ccagcttgcg tgcatccttt ccactggcag
     4381 catggccaat cctgcttcgc atcccgcttc tccgcccggg ggcgatggat gcggatcatg
     4441 tcgaagtatt tcgagtcgag cttcatggcg ccggagtatg atcgcgcggc ccggctcgca
     4501 caagtcggac tcaacgcgcg cattggccgc gtggcgatcc ggccatagac atcattgact
     4561 ccattcactg gttcctggcg gagacgaggc ccaaactgat gacgaacgag caagcgatcc
     4621 gtgaaaagct gatgctggca ctcaacccgg tgcgcctcga cgtgatcaat gagagtgagc
     4681 tgcacgccgg tcaccgcagt tcacccggta ccggccaaag ccatttccgc gttctcatcg
     4741 tgtcggaggc gatgcgcggc aagtctcgcg tggaacgcca ccgcatggtc aacgaggcgc
     4801 tggccgccga gcttaaaggt ggggtgcacg cgcttgcgat caaggcctac gcgccggggg
     4861 aagacattct gccctgatgc gcggacgcgg cctcacgcgg tcgagctcaa gccagccggg
     4921 ttgggctcgc cggcaccttt cgacgggctg gcggggatgc ttttagcggc ttcaccctga
     4981 gcgcggtgat gcggttgcgg ttcttgcgca ggatctcgaa ccgatatccg tagaacgtga
     5041 aggcctggcc cggttcgggg atggtctgcg cctcgtgaat gaccaggccc gcaatggtgg
     5101 tcgcctcctc atccggcaag tcccagccca tgtggcggtt gaggtcgcgg atcggcacgc
     5161 tgccgtcgac attcacggtg ccgtccggct ggggccggat ggcggcatcc tggacgtcat
     5221 gctcgtccga gatctggccg acgatctcct cgagaatgtc ctcgagcgtg acgagcccct
     5281 gcacctcgcc gtactcgtcg acgacgagcg cgaaatgcgc cttccgcttg aggaaggcat
     5341 tgagctgatc cttcacgccg gtcgtgtccg gtacgaacca gggctccttc agcagctcct
     5401 tgatgtcgat cttctcggca tcccaattcg cgcggccgag ggcgacgagc aggtccttcg
     5461 tatgcaggac gccgatgatg ttctcgggct cgccctccca cacgggcagc cgcgtataat
     5521 ggcccttcag gacctcctcg acgagcttgt ccaccggcat ggcggcattg agggtctgca
     5581 tcttggtgcg gtggaccatc acgtcgagaa ccttgagatc gagcaggtcg agaacaccgc
     5641 ccagcatcgc ggcatcgagc cgggtgaccg ttccctcttt ctgctgcagt tcgatggccc
     5701 cgcgcagctc ctcgtgcggc gccaggatgt tcgcatcgtc atcggcccgt ccgggcgtga
     5761 gcttgaggat gcccgagacg atgacctgca caagcgcggt gagcggccaa agcacgcgga
     5821 tcagcacgtt catgaccggc gccacgccga gcgcgagacg gtcgggaaag gcgatcgcgt
     5881 aggtcttggg cagcacctcg gcaaagatga cgatgacggc cgtcatcacg atggtcgcat
     5941 agaggacgcc gaactcaccg aaggttgcga tcagcaagcc cgtcgtcagc gacgaggcga
     6001 ggatattgac gaggttgttg ccgagcagaa ccgtcccgat gatgcgttcc ggcgcggcga
     6061 ggaggcggtt cacgagctgg gcccgccgat tgccctcctg ttcgagggcg tgcatgcgcg
     6121 cgcgggacgc cgcggtcagc gccgtctccg agccggagaa gaaggccgac gcaatgatga
     6181 gaatcagaac aaaggacgcg gtcagcagaa tctggaatgt ggtcatggtc gatcgagcct
     6241 gaactgacgg ccggcccctt ttgagctgaa tgtggatcaa gttgccgaga gccgcgcggc
     6301 gggcaaatta gccggcgacc cgcgccagaa agtccacgat ctgcgcgggc tcgacatccc
     6361 gcgtcgtgaa agcctcgcca atgccgttga tcagcacgag cgtcaaccgg ccctgccgca
     6421 ccttcttgtc ctgacgcatg aggcggacga cgtcctgggg tgccggcagg tcgccgggaa
     6481 tgtccccaat ctgggtcggc agacccaccg ccttcaagtg cgccgctaca cgcgctgccg
     6541 tctgtggcgg acagaagcgc agctcttccg agagccggat ggcgaggcac atgccgatgg
     6601 aaacggcctc gccgtgcagg agccggtccg agtagccggt ccaggcttcg agcgcgtgcc
     6661 cgaacgtgtg gcccagattg agaagcgcgc ggtcgccggt ctcggtttca tcgcgggcaa
     6721 cgatctcggc cttcatctcg acggagcgcc cgatggcatg catcagtgcg tccgggtcgt
     6781 aagcaaagag gctttgccag tttgcctcca gccacgtgaa gaaagtggca tcgccgagga
     6841 ggccgtattt tgcgacctcg gcatagccgg cccgcatctg gcgagccggc agggtcgcca
     6901 gcacggacgt gtcggccagc accaggctcg gctggtggaa ggcgccaacc aggttcttgc
     6961 cttggggtgt attgatgccg gtcttgccgc cgaccgatga gtcgacctgg gcgaggagcg
     7021 tcgtcgggac ctgaacatag cggatgccgc ggcggacgat gctcgccgca aaccctgcga
     7081 ggtcgccgat tacgccgccg cccagagcaa tcacggcatc gccgcgctcg agccccatcg
     7141 tcaggagctg ctgacacaag cgatcgagct gggcgaagct cttcgtcgcc tcaccggccg
     7201 gcagcacgat cgagccggcg tgccgcccgg ctccactcag ccctgcctcc aatggcgcga
     7261 ggtgggcgcc ggcgacgttc ttgtcggtca cgatcccgca gcggcagccg ccgagggcct
     7321 ccccgatcag gcgcggggcc tcattcagca gtccaggccc gatgtgcaca tcataggcgc
     7381 ggtccccgag ggcgacgcgg acgcggcggg ccacgtggcc ttctccggca ccagcagcct
     7441 gcgagctctt gattgtcatc ggctggccct acccacccgt cgcctcgtga gcggcagccg
     7501 aagcaggctc gccacgggtc tcgccggcga gcggtccctt gagcagcgcg tccaggattt
     7561 catcgacgat gacgtcgtga ggcacgtcgc ggctgttggc gacgagatcg gcctctgcat
     7621 agatcggata tcgctcggcc atcagctcgc gcatgcgggc ttcaggatct gcggtcttga
     7681 gcagagggcg gttgtcccgc cgcatgacgc gccgcatgag caccggcagc tccgccttga
     7741 gccagatgga aatgccggcc cgcttgatat tctgccgcgt ctccggatgc atgaacgcgc
     7801 cgccgccggt tgccagcacc tgcgggccgt tcttcagcag ccgtgcgatg accttgcgtt
     7861 ccccgtcgcg gaaatgtgcc tcgccatgat cggcgaagat ttccgtgatg gtcttgccgg
     7921 ccgccgtctc gatttcctca tccgcgtcga cgaacggcag atcgagcctg gcgctcagtc
     7981 gccggccgac ggaagtcttg ccgcagccca tcagcccgac catgacgagc gatctcgtgc
     8041 cgagcgaagc gcgaatcgcc cggacacgct cgcacgcggc ggcgctctga tgcttcacgt
     8101 tacagccgaa tcgaaccatg acttagccgg tggatctgcc gtctgtcggt gatagtgggc
     8161 gggtgaccgt cacacaagtc gccagccgtg acaagtgagg cagaaggccg gcgccggtta
     8221 gggccgtcgt tgcttgagcc cgcgttgccg ttttggttaa tcgggccata atggcaacac
     8281 ttggagcagc aaatcgcatc ggataggttg gccgacatgc ccactctctt tcgatttctg
     8341 atgatcacgg gcatcgttgc tggcattgcc tacggcgggc tgtgggcctt ggccgagtac
     8401 ttcgagccgg tgccgcacga ggtctcgacg ccggtgccgg gcgtcaaggt tcgtcagtag
     8461 cagaaggccg ccgacggagc agaaacggag caattggggg gcgcgagaaa tgaaccgccg
     8521 gcctgctgct gccctgatgc tggcgatctg gctgacgggg gcgtgctcgg ttgctccagc
     8581 ggcgatggcg caggactcct ggcgcccgtt cgatgaccgc tttcccccat ggggcacacg
     8641 gccggaccga cgagagccgg acgatcgcga gctgtacaga cgcgatctgc cgcccccccg
     8701 aaccggaacg tatgcacctc agccacggca accggcccca atggcaccct tgctcgcgcc
     8761 cgtggaacgg tcggagctgc cgccggtgat ggccaacgac gggtcgggtt tgcccttcga
     8821 cccgtgggcc ggcatcgacc tcgccaccac agagcagatc atggcgaggc tcgagctgcc
     8881 gccgctgtcg ccatcgctga tgggactgtg gcgccgcctg atgaccgaaa ccagttcggg
     8941 accggcaggc gccagcatgg agcaattcac cgcagtgcgc gccgaggctc tttatcgctc
     9001 gggacagctc aaggagttgg aagcgctgtt ctcgaccgcg gcgcccgcat cggccgaccc
     9061 gctcatcacc gccctctcgg cgcgctacga cattgccgtg ggccggcgcg acaggggctg
     9121 cagcggggtg cgggaggcag ccgcgcgcaa gcaggacctg ccgcgccagc tcggggctga
     9181 aatgctcgtt cttgcaggct actgtgcggc cgcggccggc aacaggtcgg ccgcatccct
     9241 cgcggcggat cttgcccgag aggagggcct cgacgacgcc gggctcatca gtgcgctcaa
     9301 tgccattgcc tctggcggca agccgcgtcc gcagcgcagc gggcccatca cgccgaccca
     9361 ttatcgactg ctgcagcttg caaaggcggt cgacccgaag accatcgtcg agcgggccga
     9421 cgccgcgctg ctttccgccc tcgtgctcga tccgaccacc gatgatcggc tcagggtcgc
     9481 tgcggcggaa gcggccgcgc aacgcaacgt catcacgccc gatgccctgg cggaggtcta
     9541 cagaagccag cggttttctc cagatgatct ggcccgtccg ctggcctccg atgtcgaccc
     9601 gctcttgcgg cgcgcggtgc tgttccaggc agccgagtcg gaacgcgacc cggttcggca
     9661 ggcccagatc atccgcgcct tcgtcgacga tgcgcggcag gcgggcgtct atgtccacgc
     9721 gctgatgtcc gtggcgccgc ttgctgaggc catcccgcag tcggcggagc tggcttggtt
     9781 cggcgagacc gccatcgagg cagcactggt gtccggcaat cacggcagcg cacgcgcctg
     9841 gtggaccgct ctggccgcag gcgggcggga cggcgatcgg ctcgagcact ggctcgcgct
     9901 ctcggacatc gccaacgccg atctgcgcgc cccacgcggc ctgcacctcc ccgccgtcga
     9961 agcccttgcc ctgcgcggcg gcttcagcgc tccggtgctg catcggctgg caacggtgct
    10021 cgatgccctc gactaccagg ttccgatgca gctctggcat gtcgcgagca gcacgccgca
    10081 acccaatgac ggctacttgc cggaaaccgg cgtgctcacg gagctgaagg aggcgtcgag
    10141 ccgcgagcaa ttcgcccgaa ccgtcttgct cgtgatgcag accctcgggc cgaagggcgc
    10201 ggaaggtgcc cacatcattg cgctcggcga tgccatacgc gcgctgaagc ggatcgggct
    10261 cgaggccgag gcgcggcgtg tcgggctcga ggctctgttc ggcgcctggc cgcggtcggc
    10321 aatccattag cgaaccgccg atgccgaact ccgacaagct gatcgactcg tttctggaga
    10381 tgttggctgc ggagcggggc gctgcagcaa acaccctgga agcttaccgg cgtgatctgg
    10441 agaacttctc ggctttcctc ggaaagcgcc acgtcgagct tgccgcggcc ggacccgcgg
    10501 acatttcggc ctatctcaga ttcctgacag agcaggggat ggctgcgtcc tcgcgcgcgc
    10561 ggcggctctc cgcgctgagg cagctctaca agttcctgct ctccgagggg cgagttgccg
    10621 aggatccggt gctcggcatt cagggcccga aggtcaacca ggccttgccc aagacgctgt
    10681 cggtggcaga ggttgaccgg ctgatcgcga cggcccgcgc acgcatcgaa ggcacgaccg
    10741 gccgcgaacg cttcgtcgcg ctgcgcctct actgcttgct ggagatgctc tacgcgacgg
    10801 gcatgcgcgt ctccgagctc gtctcgctcc cccgctcggt cttgcacggc gacccgcgcg
    10861 tcctcaccat caagggcaag ggcggccgag agcgtctggt gccgctcaat gctgccgctc
    10921 gtgcggcgct cgatcgctat ctggaggtcg ggcaacatcc ggaagacggc ctcgctccgt
    10981 tggcgcggac acgctggctc tttccgtcgc ggagtgccga agggcatctc acacgccagc
    11041 ggctcgccca ggagctgaag gagcttgccg aggccgccgg gctcgatccg aggcgcgtca
    11101 gcccgcacgt gctgcggcac gccttcgcga gccatctgct cgaccgcggc gccgatctgc
    11161 gcgccgtcca gcagctcctc gggcatgccg acatctcgac gacgcagatc tacacgcatg
    11221 ttctcgaaga acgcctgcgc cggctggtcc tggagcacca tccgcttgcg ggctcgaagc
    11281 tgcggtgaga cctgccgatc agctggccga aacgcaggta atactttacc tcactctcgt
    11341 tcggcttggc gcatcatgct gcgttgcggt gtattcttga ctttcggtcg cgaccccggc
    11401 gattgtccag gccaaatgga accggccgcc gcttccttgg ctgcggcaaa ccctggtcac
    11461 tcatggatcg acagcccatc aggacgtaca tcgagttcga gaagccgatc gcggagctgg
    11521 agagcaaagt cgcagagctc aaggccttga gtagcggcga taacgccgtc tcgctgtccg
    11581 aagaaatctc caaactcgag cagaaggcgc agcagattct ggccgagacc tacgccaagc
    11641 tcacgccctg gcagaagacg caggtcgcgc gccatccgga acgcccccac acgctcgact
    11701 tcgtcaacgc tttggtcgag gaattcacgc cgctggcggg agatcgctac ttcgcggagg
    11761 atcaggccat cgtgggaggc ctcggccgat tccgcggccg gtcggtcatg gtcatcggcc
    11821 acgagaaggg ctccgatacg gaaagccgca tccggcacaa cttcggcatg gcccgcccgg
    11881 agggataccg caaggccgtg cggctgatgg atatggctga ccgcttcggc ctgccggtca
    11941 tcacgttcat cgacacggca ggcgcctatc ccggcatcgg cgccgaggag cggggtcagg
    12001 cggaagccat cgcgcgctcc acggaccgct gcctctcgct cggcgtgccc atcgtggccg
    12061 tcgtgatcgg agaaggcggc tccggcggtg ccgtcgcgat cgccacggcc aaccgcatcc
    12121 tcatgctgga gcacgccatc tacacggtcg cttccccgga agctgcggcg tcgatcctgt
    12181 ggcgcgattc ggctcgcgcc atcgatgcag ccaccaacat gaagatcacg gcccaggacc
    12241 tcatgcagct caaggtcatc gacgcgatca ttcccgagcc ggtcgggggt gcccatcgcg
    12301 agccggagaa ggcgatcgcc gcaactggag aagccatcgc aaaggcgctc gccgagttcg
    12361 aaggccagtc accagccgag atccgaagcc agcggcacga acgcttcatg cagatcggcc
    12421 gcacgctcta agctattgat ccctttgaca ttggaggatc attcgaacgc cctccggctg
    12481 tcaaaatcct gtgcactgtc gcgcttgaca tcgcggctgc tcggcgtcct attgcctttg
    12541 cgtcgcgtcg ttaactgatt ctttaccaag gcacgcacac gaatcaggcg ggcgcccaag
    12601 catggcgcca agctcgaggg cctgggacgg gggtatatgc gcggcatcta cgtcggcatt
    12661 ctgatcacag cggccgcact gctagccgcc tgcgccaaga ttgaactcgc cccgcacctg
    12721 aagcctttgt cgaaggagac gatgatgctc ctcggcagaa aggatatgcg catcgatcag
    12781 cccatattcg tccgcatctt caaggaagag tcggagctgg agatctggaa gcagcgagac
    12841 gacggacgat tttatcactt caagacctac ccgatctgca actggtcggg agacttgggt
    12901 ccgaagatgc aacaggggga tcgtcaggcg cccgagggtt tctaccgggt gacccgtcac
    12961 cagatgaatc ccaattccaa cttccacctc gccttcaacc ttgggtatcc caacgccttc
    13021 gaccgggccc acaatcggac gggcgagttt ctgatggtgc acggcaagtg caaatcggcc
    13081 ggctgctatg ccatgaccga cgagccgatg gaggagatct acgggctggc gcgcgaggca
    13141 ttcatcaacg gccaggaagc cttcgaggtg cacgccttcc cgttccggat gacggacgag
    13201 aagatggctt tgcacaagaa cagcccacac tacgagtttt ggaagacgct caaggaagct
    13261 tacgactatt tcgagctgac gcgcgtgccg ccgaccatcg cggtttgcga gaagcgctac
    13321 gtcgtgaatg caaagttcag cggctcgatc gaccccgcaa agctggaccc cgttgggcgc
    13381 tgcccaccat atcagaaaat tgagcccgag cccttcgtgc cgaccggcat cgaccagcag
    13441 atcgcggagg aaaggatcgt cgcaccgggt ccgaagaagc gcacgctcgc aagcattcgc
    13501 aacggctcgc tctcactgac gccggtcacc gccagcgtga tgcccgccga tcctgctccc
    13561 acaggcacgt ccaccacagg cgtcttcgtc aacggatgga gctccgtctc gcgagccctt
    13621 gggctggcga agtagccgcc caggccgcgt ccagccttgc atccgcgagc tcgcgcgcat
    13681 cctcgcaccc cacgccgaac ctggcatgat aggatctcgg ctgcgctggg tgcgcacatc
    13741 gaaaggaggc gtcatgcgtg caaagatcaa tggaattgaa atgacttatg aggtgtcggg
    13801 ccgaaccgat gccccggccg tcgtgttgca ccatccgctc gccaccaatc tttcgacctg
    13861 ggatgcgctg accgccgctc tggagccgac ctaccgtgtc atcaggatgg acgcgcgtgg
    13921 ccacggcaag accgaagctc cggatggagc ctacagcttc gaaaccctga ctgcggacgt
    13981 gatcggcctc ctcgaccatc tcgatctcgc aaaggcgcat ttcctcggcc tctcgatggg
    14041 tggcatggtc gggcagtacc tgggcatcct gcacccggac cggttccact ccctctgcct
    14101 cgtctcgacg accagcgctg tgccatcgca ggcttggtcg ctgtgggacg agcgcattcg
    14161 cgtggcagcc acaggagacc taagcagtct cgtcgatgcc gccgtcgccc gctgggtgac
    14221 ccccgctacc gcaaagaaca agcccgaact cgtcgctcag ctgaaggaga tgatgctgag
    14281 tacgcctgcc aagggttatg gcggctggtg ccacgcgatc cgcaatctca acatcaccgc
    14341 ccggctgaag gagatcgaac tgccgactcg cgtcatcgtg ggtcaggcgg acccggcgac
    14401 acccgttttg gcggcaaaag tgatccacga ggaaatcgcc ggctcggacc tggtcgtgat
    14461 cccgggcgtc tctcacatgc tgcacgtgga agctcccgaa gccttccatc cgcacgtgtt
    14521 ggcctttctg gcggcacaca gtcggtccgt ctgaacttcc aacgaaaaaa gcccggcgct
    14581 cggccgggct ttggatgaaa cgcgacgggc ggcgtcgcct caggcgcttt gctggctttc
    14641 ctcgccttcc tctgccggaa cctcggcggc ggcggcctcg gcgaccagac ggttggcctg
    14701 gccaacccag tcgtcccgtt cgatctgacc attgagcttc agcagctcgt ccaggttggc
    14761 gatgtcctca tcggagaacg cggcgatctg gtcgaaccgc aggacgttgt atttcttgag
    14821 ctgcgcctcg agctccggtg tgatcccgct gatctgcttg aggtcgtccg gctggccctt
    14881 cggccgcttg aagcccttga aggcggcatt caggcgacgc gcgcgggcat ccgtgcgctc
    14941 cacgatgcgc gcagccttgc cgcggcggcc gcgcaggtag tagagcttcg cgcggcgcac
    15001 cttgccgcgg cgcagcacct caatgtcggc gatgtagggc gagtagacgg ggaaaacgcg
    15061 ctccacgccc tccccgtacg agatcttacg gaccgtgaag ttctcgttga gcccggagcc
    15121 ggagcgcgcg atgacgacac cctcgtaggc ctgtgtgcgg acgttgtcgc cctcgatcac
    15181 cttgaccatc accctgacgg tatcgccggg accgaactct ggaacgacgc gtttgctggt
    15241 gaccgcgctc atctgctcgg actcgagcac ttggatgatg ttcatggctg gacctcgacc
    15301 ggagtgtcaa tgcgcgccac gggctccgac aagcggaccg tcgaccccat gcggcagcat
    15361 ttcgggaatt cggatcgcgg gtagataacg caatcggacc ggcttgtcga ctctcgcttt
    15421 gttaggatta cgtcaggact tttcaccggc cttggcaatg agatcgggcc ggcgctcgcg
    15481 cgtgatcctg agccgctgct cctggcgcca ctcggcgatg cgcttgtgat cgccggagag
    15541 caggacctcc ggaatggatc gcccttccca ttcccggggt cgggtgtagt ggggatactc
    15601 cagcagattg tcctcgaagc tctcctccga gagcgactcg tgcgctccga caacgcccgg
    15661 cagcagacgg acgcaggcgt cgatcagcac catggcagcg agctcgccac ccgacaggac
    15721 ataatcgcca atcgagatct cgcgtgcgcc tcgcgcgtcg agcacacgct ggtcgacgcc
    15781 ctcgaagcgg ccagccagca ccatcacccc cggtcccgca gccagctcgc gcacgagtcg
    15841 ttgggtgagt ggccggccgc gaggcgtcat gtagatgagc ggagcgtcgg gttgctgctt
    15901 gcgggccgcg tcgatggccg cagctgccac gtcgcagcgc atcaccatgc ccgggccgcc
    15961 gccggccgga ttgtcgtcga cggtccggtg ccggccgatg ccgaaatccc gaatattgta
    16021 ggtcgcgagc gaccatacgc ccgagtcgag tgcctggccg gacaatgaat aaccgagcgg
    16081 cccgggaaac atttcgggaa agagggtgag aacagttgcg cgccacggcg ccatgccggt
    16141 ggcgtcgtgc ggcgttggcg ctgacatttg cggctctccg cgggcttgct gatgcgggct
    16201 gctgcctgtt ctccgatctc aggggccaag tcaatggcgc caagggcgaa agcccatgac
    16261 caagccgacg cccgaatccg gctcaggctg ccaacgggcg gacctgctct ggcagaatga
    16321 gttcggccgg ccgcggcgag tctcccacct cctgcgcctg cggcaggttg atgacctccg
    16381 cgacctccag caggagccgc tggaaggcgt agtccgcggc ctcgagccgg acgagagagc
    16441 gctgatgcag ctcacgcgcc cgctcacccc tggcaacgac ctcatggatg agcgcggcgg
    16501 cacgttgaga gtgcgcttcc ggagagatgc gcggcggcac ccattgggat gcggacggcg
    16561 ctaagaattc ctgcggggcg gctttcgaag gtctggtggc ctttgccgcc gccttagagc
    16621 ttattctgcc tgccgccttc gatcgcaccc tgcgagcgct ctggcgcttc ggcctgctcc
    16681 ttgactgcca ccccagcttt ttcatcaggc gcatcttcag ccaataaaag attccgacga
    16741 gaagggctga agccgcaaag acctgcccaa agccgaccag cgccgcctgg tagtcgcgtg
    16801 caaagtcgta aagcagtgac atcgtccggc aatgagggtt cgggaggacc agaaggtgaa
    16861 acaatacacg ctggaaaagc ttcgagaatc gataaagttc cagatgccgg agtaggctct
    16921 gagaagtctt aatgaactct taacttctgt tcagaagcag ggcacgacag cgtctgcgtg
    16981 ccgcctatcg cgtctgcttc aggagctgag accgatcagt cgtcggatat cgtgcggggt
    17041 cagtccggcc gctctcagcg ccttgagcga cgtatcgcgc gcactcttgc tgagcttgcg
    17101 tccggtcgcg tccgcgatga gccggtgatg gtggtacacg ggctccggca aatcgagcaa
    17161 gacctggagc aagcggtgca cgtcggtcgc cgcgaagaga tcgaggccgc gcgtcacgtg
    17221 ggtcacgcct tggcgcgcat catcgacgac aacggccaga tggtagctcg caggaatgtc
    17281 cttgcggacg atgatgacat caccccagcg ctcgggccgc gcccggatgc gacggatcgc
    17341 acgaccatcg gcgtcgagct cggcaaacga cagctcgctg ctccccgttc tctccttcag
    17401 cacttctaac gccctggcca tgtcgaggcg catggcgaac ggctggccgg cagcccggcg
    17461 gcggatttgc tcctcagggg gcaatggaag aagagcacgc gtgatccggg gggcgccatc
    17521 gggatcgacg agttccggat gctcgatcgc gaacgcgcgc aactcagcgc gggtcaggta
    17581 acagggatag agcaggcctg tgcgcttgag gacttcggca gccacccggt agtcgtcgaa
    17641 gtgctgcgac tggtgcagga ccggctcttc ccactccagc ccaagccagg ccagatcctc
    17701 gaagatggcc tggacatagt cgggtctggt gcggttgacg tcgatgtcct cgatccgaag
    17761 cagaaaacgc ccgccaagat ggcgcgccat ctcgaacccg gtcagcgccg acaacgcgtg
    17821 gccgaggtgc agctcgccgt tggggctcgg cgcaaagcgg agcacgggtc tcgtcatgcc
    17881 gcgtgataaa agcacgaccg ttatcctatc tccatcgccg ggccgaatgg cgctaagctg
    17941 ccgatctcat caacttgtca caagcgcaca agcggacggg aatggctaag ctgcgaaatt
    18001 ccagcagtcg ggcggacgtg gacgtgggca tcatcgagac cgccgaggac atccgcgccg
    18061 ggatgcgggc gatacggcgt aagtgccccc atctgcggcg cgtccacgac gtcacgggcg
    18121 atcccccgct gcggcggcga ccggcaggat tcgagggtct tgcgcgcatc atcgtcggtc
    18181 agcagctttc gacggcaagc gcggcggcga tctggtcgcg gctcgagctc gcagtcgctc
    18241 ctctcgcccc tgaaactctc ctagcggcga gcgacgaggt gctgcgcagc gccggtctat
    18301 cgacccccaa gctgcgcact ctacgcaatg tggcccgcgc cgccgccgag ggcgagcttg
    18361 cgatcgagac gctgacggcg ctgccggatg aggccattca cgagcaattg acccgaattt
    18421 ccggcatcgg cccgtggaca gcggacatct tcatcatgtt ctgcctgggc cggcgggatg
    18481 cgttcgcagc aggtgatctc gccctgcaag tggcggccga aggggccatg ggtctcgaca
    18541 gccgccccag tgccgccgag ctgctcgcca ttgccgagcg ctggcgtccc tggcgcggcg
    18601 tcgcagctcg gctgctgtgg gcctaccatc ccattctcaa gaaaaggggc tctggcgcgc
    18661 cgctctgagc gacacccatg cgacatcttg acggacctcg cctggagccg agccgacccg
    18721 tgacgggcct tgtcgtcctt ctgcacggct atggggccaa tggagaggac ctgatcgggc
    18781 ttgccgaagc ctggcagccc ctcctgccgc aaatcgcttt cgtcgccccg aacgcgcccg
    18841 agcctttgcc gggggcaggc ttcagtgcgc gccaatggtt cgagctcacg tttcgggatc
    18901 tccacgagct ttggcgcggg gtcaatgccg ccggtcccat actggagaaa tttctcgatt
    18961 cggagctcgg ccgctatgga ctgccgccaa ataggctggc gctggtgggc ttcagccagg
    19021 gcacgatgat ggcccttcat gtggggctgc gccgcgcagc cgcgcccgcc gccatcatcg
    19081 gctattcggg agtcattgcc ggaccggagc acctcgaagc tcagctgaag gcccgtccac
    19141 cggtgcaact catccacgga gagctcgacg acgtcatccc catcgaggcc ttacacatca
    19201 cgcgtgaagc cctggcccgt gtgcggatcc cagtcgaatg gcacatccgc cctgggctcg
    19261 gtcatggcat cgagcccgtc gggctcaagc tcggcggcag ttttcttgcg caggcactga
    19321 agcccggcca ttagggaaag ggcacaggcc gaagctggta tccgccctcc cgaaatttga
    19381 atcagttgcg cttttccgca cacttcacag aaccgacatg cagcgatagt atccctggtc
    19441 agcgcgagtg cgacgtgcat tgctcgaacc gctagggcag agtacgggaa gcacagacgt
    19501 gacagcctca gcgacaccgc cggaatcaat gccggctctg gtcctgaacg ccgacttccg
    19561 gccactcagc tactatccgc tttcgctctg gagttggcag gacacgatca aggcggtctt
    19621 cctcaaccgg gtgaacatcg tctccgaata cgagaagacg gtgcgcagtc cttcattcga
    19681 aatgcgcatc ccgagcgtcg tcgcactcaa gaattatgtc aggcctgccc tttatcctgc
    19741 cttcacgcgg ttcaacgtct tcctgcgcga ccgcttcacc tgccagtatt gcggctccaa
    19801 agaggacctg acgttcgatc acgtcattcc gcggtcgcgc ggcggtcaga cccgatggga
    19861 caacgtcacg acggcgtgcg cgccttgcaa tctggcgaag ggcggagcca tgccggacaa
    19921 ggccggcatg cggccgaagc aaccgccgta tcgtcctacc gtcttcgagc tgcatcgtaa
    19981 cggcaggctt ttcccgccca attacctgca cgaaagctgg ctcgattacc tctactggga
    20041 taccgaactc gaaccctgac gaacggctcc gacagctcgc gcgaccatca tctacgccca
    20101 atcgcgcgat gcaatatcca ggtcggcacc aacgcgacga ggaagatcac catcaccacg
    20161 aggaagccgt cgcgaaaggc gagcgtattc gcctggattg caatcatttg gccgagatac
    20221 cagagcgctg cgggaatctg ctgaatatcg ggcaagccgg catgtccgag aagtgctgcg
    20281 acctgatgca gaagcgcaac cgtcgtggaa ttgtcgctcg tttgggtcgc cgccagtgcg
    20341 tcggcgtgaa aaatcgtgcg gcgctcgaga aacacggaca gcagattgac gccgaaggcg
    20401 ccacctagct ggcgcacgaa gttgatggca cctgaccctt gcgcgatcag ctcgcgcggc
    20461 agcaccctca gcgacccggc cgtcagcgcc gggaacacga ggccgaggcc gatgcgcgag
    20521 atgacggtcc accaggcaac cgtccagaat gccgaattga tctcgatgga cgcggtcagc
    20581 caggacgagt aggcgaaaat cgccatgcca acgccgatca gcaggccaat gggaagccgg
    20641 tctgccaggc ggcccgcaag cggaaagacg aggaccagga cgaaccctga gggcatcagc
    20701 agcagcccgg cctcggtcgg cgtaaagcct tgtatcgtct gcacgaagag cggcacgaga
    20761 tacgtcgagc cgaacagccc ggcacccatg atgaagctga cgagcgcggc ggccgcgaat
    20821 ggcacctcca ggaagacccg caggtccagc atgggctttt ctgccctcaa ttcccaccac
    20881 gcgaagctcg ttatggcgac gacggaaatt gcgaactgca gaagaatggg atcggagttc
    20941 cagccctggc gctgcccatt cgtcagagcc gtcagcagca tggacaggaa caggccgagc
    21001 agccccatcc cgatccagtc gaagcccggt cgcggcccgc tttgctgtcg ggtgggcagg
    21061 aaaaagttcg aaaggacaac gccgaggatc gcgaagggaa cgcccagata gaagacatag
    21121 cgccagttga acgtgtcgat cagcagcccc ccgatccacg ggcccaacgc cggcgcgagc
    21181 acgacgccga tgccatagat gcccattgcg gagccgcgct ggtccggggg aaacacttga
    21241 aagagcagca ccatcccgag cggctgcacc acgcccgccg ccgcaccctg aatgacgcgg
    21301 gccagtgtca gcacctgctc attcggcgcc atcccgccga gcaccgaccc cgccatgaag
    21361 gtcaagagcg cgccgatcat gctgaggcgc tggccgaaag ccttgtcggc ccagtccgtc
    21421 agcagcatcg tcgccgtcat ggcagcgagg aagccggtcg acagccattg cgcctcgacc
    21481 tggctgattc cgaacgcgcc catcacgtcg ggaatggcga cgttcacgat ggtcgtcgaa
    21541 agcacgaccg caatcgccga gatcatcacg ctgcacgtcg cgagccaacg gtaggccggc
    21601 ccgtagcggg cgaagagatc ctcagtcgac gacatcgatg ctgacttcca ccatcatgcc
    21661 cgggcgtaac agcccgtcct tctgctcgac ggagacgcgg atggggacac gctgcgtgat
    21721 cttggtgaaa ttgccgctgg ggttcgggct gggtagcaag gcgaactggc tcgtggcggc
    21781 ctcgccgagg cggatcacct tccccttgaa ctccagatca gaataagcgt cgaccgtaat
    21841 cgtggcgggt gcgccgagct tcagccgact gaagtccgtc tccttcacgt tggcatcaac
    21901 ccagacattg gatgggtcgt ggtagaccag caggcgcgct ccaggtgaaa catactcgcc
    21961 cgggtcgacg aaggtagcgc cgacgacgcc gtcaaaagcg gctcggatcg tgcctcggtc
    22021 gatctccaca tccttgcgat cgcgctcggc gatctgggct gacttacgcg actccagaac
    22081 tgcgatctgg cgctccagaa cctcgatctc tgcggcttgc gcctccacaa cggccagatt
    22141 ggccttggcc gtggcgatcc gggcttcggt cgcattttgt tgctgctctg ccgtggtacg
    22201 ctcggccagc gcctcctcca ggttttcctg cgcgacgacc ttgcgctgtg cgagggcctc
    22261 gatccggtcg tagtggctgt gcgccttctg cagaaccgcc cggctcgccc gatacgccgc
    22321 ttcggcggca gccacctcag cctttgctgc atcgaccttc ttcgcgatct gcacgcgaat
    22381 catgtcctgc tgcgcacgaa gctgcttctg ctccgcctcg atcgcggcga tctgagcctc
    22441 tatggcgtcc cgctcgagct tggtctggct gcgatcgatc ctgacgagaa gatcgccctt
    22501 cttcacggca tcgccgatga caaccggtac ctccagaact cgtccgttca cctcactgct
    22561 gacggttatg aggttggcgg caatgcggga atcgtcgatg tagacatggg tccagcgatg
    22621 cacgagccaa tcggcggcaa ccgccaggaa gagcagggcg atgacaccga tcgcactcat
    22681 gcggaaccgg cggcgctgcc agaagggcag cggagccgaa aaggcctttt ccgctttggg
    22741 ttcggcgatc gaaggggctt ccttcgtccg caagtccgtg actgcgctca aattcctgcc
    22801 ccctccagtc tgtcggcaat cgtcttgagc accctgccgg cgatggcgag atcgccggca
    22861 gggatgccgg caaggagctc gccccgcagt tctccggcaa tgcgattgat ttcggcgagg
    22921 gtcggcccgg cgcgttcggt gagcgcgacc ttcttgactc ggcgatcgct gccgcaggcg
    22981 cgcctttcga ccaggccctg ctgctccagg ccatccagaa ggcggacgag ggtcggccct
    23041 tcgatgccga tcatgtcagc gagctcccgc tgggaaatcg gcccagatcg agagaggtac
    23101 agcagagcga cccagcgcgc ctgggtgagg tccaggtgcc tcaatcgctc gtccagccgg
    23161 gcacgccacc ggcggctgac acgcgacaac tccaacgcga actcttgcgc ggtgctcata
    23221 tgaataggat tctaatgatt agagttctat catacggcgc gagcactcga cccgcaacgc
    23281 tctcccgcac cccccagcat gagcaccaag cgtgcaacga aaaatgcccc ggttggacgc
    23341 cggggcagag gtaacttcat caggaaatcg tagaagatca gggctgtttc aggccgccat
    23401 tcggaaattg cgaagacaga gccgttcagc gatcacgtcc actgccttgc tccagctcag
    23461 cccctcgacg tgctcgagac cgaaagcggt aacggcgtcc gcgggagttc ctccgtcgcg
    23521 gaagacccgc gcgcagatct ggcgcgcgac accaatggcg gaatgccact gcagcggatt
    23581 gatgttattg cgaatcatga cttgcctcac atctcgacgc ggctcattgc cggaccatca
    23641 gggcggcaaa cacgttcgac gcttggcatc actacgcgag accgtgcaat gtgacgcctt
    23701 cgggagactg cagtcatgcg cctcacgtgc ttgccaactc atggggggcg gggggcgccc
    23761 ccaattccgt cctcagtttt ccaccggagg aggtgtcggt tcggcgctga tgagcttcgt
    23821 ctccagacaa cgggagggcc ttgcgttcct tgctcagctc tctcagcaac gatccgacta
    23881 cctcgtgcag tggaagccac gggctttggg cctgaccaac tgcgtgcata ctcgaaacgc
    23941 ctcacgagac tcgatgaacc tgcagacgca gaactcctgc tgcagaacgc aaactctacg
    24001 cagacgccaa tccacctact gcagacgcga aacgattcac tgcagacgcc gactcactgg
    24061 gacacggggc actttttcca ccgcctgcgg tacgctgctc tccaggaact ctctggagcg
    24121 ggccgactga tccgccgctt gtgcgagccg cacagcgata cgcctacgtt ttctcgccgc
    24181 tgcggcccgc ttgagcttga gaaacgcgct gcggagcgcg cttcttggag aagtgccata
    24241 cgccaactca acagaacgca acggaacgcg actggtactc gctcgcggca acagcctcgc
    24301 gaactctgcg gccgttgctc cggacgccgg tacgctggca tcccgcgcga tacgcctgaa
    24361 caaggagcgc ggcagcggca tacgggagga agcgatacgc gatacgctgt gcactggagt
    24421 tacgctcctc acctaaaaca cttacgttcg ttctgtgttc cgcgaacggt ctaaacttaa
    24481 tccttggcat gtggctgaac ggtgacacgt ttttggccaa gacaaggttt ttcactgagg
    24541 atttgttcag ctctgaatag cgctgaatac gcgcctgcgt cggcgcgcgg gcgttctcag
    24601 gcccgctgcc agctgagacg cgctgccgtg aagatggaga gcgaaaggag cagcgacagc
    24661 acggcgttcc agccggcgaa cgacaggccc aggaaccgcc acgccgcctc gtcgcaacga
    24721 acgacgcgca cgctctcgag ccgcttcagc agcccgccag ggccagcccc aagcggctcg
    24781 agcgcgaact ggcaggtctc aggccccgcc cagaatttcc actcgacgcc ggcgtgatag
    24841 accccaagcc ccgcgttcac gaggaagcct atagccacaa gcgcgaaaag ggcggctgca
    24901 agccgtcgct gtccggtgct gacgaggacg agggcgccga acagcagcgg aatgccgatg
    24961 tagtaggcat agcgctcctg caagcagagc gcacaaggcc gatagccgcc gatgtgctcg
    25021 aacccgagcg ccgtcaggat gatcgccact gccgccagga gcgcagcggc gccccatctg
    25081 taggctggat ctcgctcgga aacgggcagc ggcgatggca tgactcacac gagatagcga
    25141 agggtaatga acccaccgaa tagcagcacg aaaaacacgg tcgtcaccag ccccagccgc
    25201 tgctcgataa attcgcggat gggagcgcca aaccagtaga ggagaccgct gacgagatag
    25261 aaccgcagtc cgcgggaagc gatgctggcc agcacgaata tcacaaaatt gagctgcgtg
    25321 accccgctgg caatcgtaat caccttgtag ggaaagggcg tcacgccgaa aaagaagacg
    25381 atgagtgcgc catactcatt gtaggtgctg gcgaaccgct cgaactgctc cgcgtagccg
    25441 tagagatcca aaagcggctt gccaagcagg tcgaacagga agagcccgat caggtaaccc
    25501 gcagcacctc ccagcacaga accgacggta gctatggtgg cgtacgccca ggccctcatt
    25561 ctcttggcga ggaccatggg catcagaagg acatcgggag ggatcgggaa gaacgaactc
    25621 tcgatgaacg acacccagaa cagcgcgtgg ggagcccgcc tgtggctcgc caagtccatc
    25681 gtccagtcgt agagttttcg cagcatggcg gccgtgatta cgctgagaac cacggcctgt
    25741 ccaccaaaca gaaccgctga tcgtggtgat cctgtcagac cggaagaggg tcgtcgaagg
    25801 ctgccgacag acgcaagacg agctcgttca gcaccatacg gcccgacgga gtcgccttga
    25861 tgcgcctgcc gccatccaca acggcgatga gaccctgtcg gcagagctct gcaaccgtgc
    25921 cctcgcgggg acggaccccg ccgattcgcg ccagccgttc gagatcaagg ccttccgcga
    25981 gccggaggcc catcaggagt gcctcgtcgg cctgctcgcc ccgactcagc ggttcggcgg
    26041 cgatcagccc atgcccgaat tgctcgactt tctccagcca gcgctcgggt gcccgctcgt
    26101 ccatcgttgc aagccgctcg cgccctacga tcagccggcc gtgggcacca gggccgatgc
    26161 cggcgtactc gccatagcgc cagtagagga gattatgccg gctttcctcg ccgggcactg
    26221 catgattgga aatctcgtaa gcgggcaagc cgcgtgcaga catgatctcg ttcgtcagct
    26281 catagagcgc gctcgcctgc tctccatccg gcacacgcag cttgccggca gcatgcagcg
    26341 ccgcgaatgg cgtcccatcc tcgatcgtga gctgatagag cgacatgtgg ccgccggcta
    26401 tgatgatggc ttcctcgagc tccgcgcgcc aggcctccag cgtctggccg ggccgcgcgt
    26461 agatcagatc gaagctgaag cgcgcgaacg tgctgctggc gaccgagagg gccgccttgg
    26521 cctcggcgac ggtgtgcagg cggcccagtt gcctcaagtc cacgtcgcgc agcgcctgca
    26581 ccccgagcga gacgcgattg acgccagcgg cccgatagcc gcgaaaccgg cctgcctcga
    26641 cgctcgacgg gttggcttcc agcgtgacct cgacatggct gtcgatggac cagagccggc
    26701 cgatctcatc caggatgccg gccaccgtgc tcggctgcat gagggacggc gtgcccccgc
    26761 cgatgaagat gctgttgacc gtgcgccggc cgatgcgctc gcgcgtcgtc tgcagctcgc
    26821 ggcggaatgc cgccaggaat cgcggctcgt cgacgccgct ggcgcgcacg tggctgttga
    26881 agtcgcagta ggggcacttc gacgcgcaaa acggccagtg aatgtagacc ccgaagcccg
    26941 cctcgtcagg attgccggcc atcgaggcag gcatcgacaa agagcgcgaa agcccgggcg
    27001 cggtggctga ttgcatgttt ccttgccgga tccatctcgc cgaacgtcag ggtctcgccg
    27061 tcgggcagga acatgggatc gtaaccgaac ccacgactac cgcgcggcgg ccacacgagc
    27121 cggccaaaaa cctttccctc gaagatctgc gcctccccgt ccggccacgc aaggctcaag
    27181 gcgcaggtga aattggcacg cggccccgcg ccgctccacg cgcccactgc cgtgagctcg
    27241 tccgcgacgc gctgcatggc gaggctgaag tccttggccg gaccggccca tcgggcggag
    27301 tagatcccgg gccgcccgcc caaagcctcg acttcgaggc cagagtcatc cgccagcgcc
    27361 ggcagccccg atcgggtcgc agcggcgacg gctttcaggc gcgcattgcc ggcgaacgtc
    27421 gtctcagtct cctccggctc cggcaggttc aggtctttgg ccgagacgac ctccaggccg
    27481 taagggccga cgagatcagc aatttcgcgc agcttgccgg gattatggct ggccaccacc
    27541 aaccgcgtac ccttctgcag cttcctcatc actcgagacc ccagatccgc ggctcggcga
    27601 actccaggca attcccggcg ggatctcgga agtagatcga gcgcccgccg cccggccact
    27661 cgaaatcggc ctcgatgggg acgccgcgat cgatcagtcg ctgccgccag ctctcgatct
    27721 catcggcgct ggcgccgaaa cagacgtgac ccggcccgac ggcgccgtgc ggagggactg
    27781 gcagggcatt cggccccggc ggcttcgagg tggcctgcgg gttgaacagc agcagaacct
    27841 gattgccaca acggtagaag agctgccgtc cgcgggcctt ggagaacggc tccatgccaa
    27901 gcacgtcacg atagaaccgc tcggcggcct ccaagtcgct gacgtaaaga accgtttcca
    27961 atatgcgcgc cggagtcatg ccttcgcctt cgctggtgcc ccggcgatga aacgctcaag
    28021 ccaccgtcag tttctgaagt cccaccagct ccttgatacc cttgcgggcg agcaccatca
    28081 gctcgtcgaa ctgctcctgg ctgaacggct ctttctcggc cgtaccctgc acctcgacga
    28141 tgccgcccga gccggtcatc acgaagttgg catccgcatc ggcctccgag tcctccgcgt
    28201 agtcgaggtc gaggaccggc tgcccctcgt agatcccggc cgacacggcg gcgacctgat
    28261 ccttcaagac gtcgccgccg accatgtcgc gcgcccgcat ccattggatg cagtcgtgca
    28321 gcgccaccca ggccccagtg atcgccgccg tgcgcgtgcc tccgtctgcc tgcaacacgt
    28381 cgcagtcgat ggagatttgc cgctcgccga gcttctgcag gtcgacgacc gagcgcaacg
    28441 cccgaccgat caaccgctgg atttcatggg tccggccggt cgcatgtccg atggtcgcct
    28501 cgcgccgggt ccgctcgctg gtggcgcgcg gcagcatgcc gtactcggcg gtcacccacc
    28561 cacgcccctg atttttcagc cactgcggga cgcgctcctc caaactggcc gtgcacagca
    28621 catgcgtgtg cccgaacttg atgagacagg agccttccgc gtgcatcgag accgcgcgct
    28681 cgaacgagac gcggcgcaat tcgtctggct tgcgttttga cggtcgcatg acaatgttct
    28741 gggttgcacg agcaggccag ccgaccgccg gccaacccct ggcgcggcaa actaggcgag
    28801 caggggccgc cgttcaactg catttcgcgg catttgacga gcaggtcggc gtcggcctag
    28861 attcgagcac aaggttgttg gtggaagctg tacaccttca acgagaacca tgacaggaac
    28921 gattagcgag ctgaacaagc tcaacgagcg ctcccgcgcg atatttcgtc aaatcgtcga
    28981 gaattatctc gagaccggtg aacccgtggg ctcgcgcaac atctcgcgca atctgccgat
    29041 gacgctgtcg cccgcgtcgg tgcgcaacgt catgtcggat ctcgagcagc tcgggctcat
    29101 ctacgcacca catacgtcag ctgggcgatt gccgacgcag atggggttgc gtctcttcgt
    29161 cgacggcctt ctggagctgg gagacctctc cgaggaagag cgcaagcaca tccagtcgca
    29221 gatcgccttc aggcgcgaca agtcgatcga gcagctgctg gaggaggctg gagagatgat
    29281 ctccggcctg tcgcactgcg ctggcgtcgt gctcgccgag aagcaggtga gccggctcaa
    29341 gcacatcgag ttcgtgccgc tggagcccgg tcgcgcgctg gtggtgctgg tgggcgacga
    29401 ccagacggtc gaaaaccgga tcgtttcggt gcccgccggc ctgccgcctt cggccctggt
    29461 cgaagcagcg aactatctca atgcccacgt gcgcggactt tcgatcgacg aggcccggag
    29521 ccggatcgag cagcagattg cctcggcccg agccgagctc aatacgctca ctcagaaagt
    29581 gatcaaggcc ggtctggcgg aatggtccgg ctcgctcgga gaccggcaga gcttgatcgt
    29641 gcgcggccag tcgaacctgc tgaaggacct caccgccatc gaggagctgg agcgcatccg
    29701 ccagctcttc gaggatctcg aaaccaagcg cgatctcatc cagctcttgg gccttgcgga
    29761 gcgcggcgag ggtgtccgga tcttcatcgg gtcggagaac aagctcttct cgctttccgg
    29821 ctcgtcgctg atcgtggcac ccttccagga caagtcgcgc aagatcgtgg gcgtgctggg
    29881 cgtgatcggg ccgacacgca tcaattatgc ccggatcatc ccgatggtcg actatactgc
    29941 caagctggtt ggtcggctgc tcacttgatt tttctttccc tcaacccatc ataccggccg
    30001 actctcgagg cgagcagacc gccagcctcg ctttttgcgt actctccaga acagctctga
    30061 ggactcatga gcgcaaagcc tagcccagaa gaacaaccag ccccgcacga ggcgtcagcc
    30121 gaggcgcaaa aatccgaggc ggccgccgcg gaggacaatc ggccgcaggc gggttcaaac
    30181 gagcaaggcc cggcgacagc cgaagccggt agcgagcggc ctgcaaagca ggaggcagcc
    30241 gccgccgaag ctgaggcggg gcaatcggcg ggtgccgagg cacgggtggc ggagctcgag
    30301 gcgcaggtgg ccgatttgac ggatcgtctg cttagggcac acgcggaaat tgacaacatc
    30361 cgcaaacgcg ccgagcgcga gaggttggac acggccaagt acgcgatcac caagttcgcc
    30421 ggc
//
LOCUS       DASVDU010000010        15799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_558641, whole genome shotgun sequence.
ACCESSION   DASVDU010000010 DASVDU010000000
VERSION     DASVDU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15799
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558641"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..929)
                     /locus_tag="VF226_00745"
     CDS             complement(42..929)
                     /locus_tag="VF226_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7072546.1"
                     /translation="MRRLRADLLLLLAAAVWGVAFYFQKAAMEHIGPFLFIAARGLIA
                     AVCLTPLALIEARRVANSWSSGLVQIGVAGGLAFLVGASLQQTGLIDATVTNAGFLTG
                     LYVVATPLMAWIAFKQRPAVYVWPAVALAFLGVWLLGGGTVGGFSTGDGLIAASAVAW
                     AIHMLITSRATIYGRPVAYTAMQFACVAVGGGILTLAIEHNTLDGLYKALPAVAYVGV
                     LSSALTFTLLAIALKDTPPGEASILMSMETLFAAAAGVLFAGEWLAPIGWVGAALMFS
                     ATILVQIGPMLESRHTKTL"
     gene            1076..1441
                     /locus_tag="VF226_00750"
     CDS             1076..1441
                     /locus_tag="VF226_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX7072547.1"
                     /translation="MPAAGSLSWPHIAPLVLLLASNVFMTIAWYGHLKFKTAPLLVVI
                     VGSWAIAFVEYCLAVPANRIGHAVYSAAELKTMQEVITLVIFAAFSVLYLGEPLTWRH
                     AAGFALIALGAGFVFSARA"
     gene            1446..2201
                     /locus_tag="VF226_00755"
     CDS             1446..2201
                     /locus_tag="VF226_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7072548.1"
                     /translation="MPEPVILLVLMAAAFAGAFVQAAVGFGFALIAAPIFLTAMKSGS
                     AMQVLVAIHVVQSAMLVPGLWRSAPAKLLHPMLLGSLAGFPLGLAVFVALDLDALKLA
                     VGMVMLVFTALLVARDRSWLSQMFASSGPPNRAAAAGVGASAGALTSVLVMPGPPLIL
                     YVAARRIGKEESRALSLTFFAFCYVAVTLLHATWTGMSFETWVLAAALSPVVVIGTLL
                     GDRLARWLSEAWFRTAVLAVSFLSGAWAVWSSF"
     gene            complement(2423..2602)
                     /locus_tag="VF226_00760"
     CDS             complement(2423..2602)
                     /locus_tag="VF226_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072549.1"
                     /translation="MRLNPPTIAVFLISLVLALLAVATKLGFLHVPRYLPHQEYWLAI
                     TAYMVLMVGNLVRGL"
     gene            complement(2637..3137)
                     /locus_tag="VF226_00765"
     CDS             complement(2637..3137)
                     /locus_tag="VF226_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072550.1"
                     /translation="MVNGRKIIAGLPLIALASACTAVGVLAWTVFRPDAILDRGVEAA
                     LAESPVAQAPDTPPNTQRKQALAESPNFWLSRLDDELPLGLSKPVQVGDRIEISGGNG
                     NRTLEVIDIREIGPNLTRVESKAGPRFLLVTTRETGTSHPRLVRFIIEADELSPMGIG
                     ETHRTL"
     gene            3400..4200
                     /locus_tag="VF226_00770"
     CDS             3400..4200
                     /locus_tag="VF226_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX7072551.1"
                     /translation="MKLTPEQLKQFEDEGWFFLPDCFSEEEVSVLRREAEQIFSQSRQ
                     EIWREKSGAPRTAFAAHTYNEAFGILARHPRLVEPVEQVFGEKLYMHQFKVNAKAKFD
                     GDVWQWHQDYGTWARDDGMPEPRAMNISVFLDEVFTFNGPLMLIPRSHKHGVLEAGHD
                     KATTSYPLWTLDHETVERLCNDGGLVVPTGKPGSVLMFHGNLVHGSAGNITPFPRKIV
                     YLTLCAVSNYIRKPTRPEWIAHRDFTPIETCADDALLTYARQYRQAAE"
     gene            complement(4290..5570)
                     /locus_tag="VF226_00775"
     CDS             complement(4290..5570)
                     /locus_tag="VF226_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEX7072552.1"
                     /translation="MAEMILPHSVEILAAALFALAVIHTFSTAYFEHLAKVQPAHAGL
                     WHLLGEVEAVFGFWAMVLLVLVAIQLGTDSSMHYLESRNFTEPAFVFVIMVIAASRPI
                     IDFAGALLVWISRILPLPASAAYYLTVLAIGPLLGSFITEPAAMTLSALLLRERFINR
                     NAPNVFKYATIGVLFVNVSIGGVLTPFAAPPVLMVAGTWGWDMAFMLLHFGWKAAIAV
                     FINAVVVTYLFRNHLKSVQTMSAALARLDVPSWVVAVHLMFLTGVVVFNHHLVVFAGI
                     FLFFLGFTEAYKQYQSRLLIREGLMVAFFLAGLVVLGGLQKWWLQPTLEGMSPAVLYW
                     GATALTAITDNAALTYLGSLVEGVTDDFKYALVAGAVTGGGLTVIANAPNPAGFAILK
                     GSFDDGAIKPLGLLAAAVGPTLVAAAMFLLLPTL"
     gene            complement(5768..7942)
                     /gene="uvrC"
                     /locus_tag="VF226_00780"
     CDS             complement(5768..7942)
                     /gene="uvrC"
                     /locus_tag="VF226_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7072553.1"
                     /translation="MPQDRVSEAREAAVQERTSPKTGPEVIASYLKNLPNSPGVYRMI
                     DAKGDVIYVGKARSLKARVANYTRFAGHSTRIARMISQTAAMEFVLVGTEAEALLLEA
                     NLIKRFRPRFNVLMRDDKSFPYILIARDHRAPQILKHRGARTRRGEYFGPFASAGAVN
                     RTITMLQRAFLLRSCSDAVYETRTRPCLLYQIKRCSAPCTGEISLEDYNALVDEATRF
                     LRGESQNVRRHYQKLMEEAAEALEFERAAELRNRLWALAHVTADQAINPEGVEEADVF
                     AIAQDGGQTCVQVFFIRSGQNWGNRAYFPKADRSLGTAEVLESFIAQFYDDKPVPRLL
                     LLSENLPGRALLAEALSSKADRRVEVRAPQRGSKLGVVEHALRNAREALARRLAESSS
                     QRILLEGIAERFGLPATPRRIEVFDNSHIMGTNAVGAMIVAGPDGFVKSQYRKFNIKS
                     ADLTPGDDYAMMREVLTRRFKRLADKHAGERPAAREPQPVSGHSISPPDGEPATRIEP
                     PAPDPVTRRGADGIPFGMLRAETVSEPGLENHGENRPEPPDCSPALFDETPAEREPEP
                     AEEEFPDRPDLVLIDGGAGQLGVAADVLRELGIEDIPIVGVAKGPDRDAGREHFYLPG
                     REKPFMLEPRDPVLYFVQRLRDEAHRFAIGTHRARRAKAISANPLDEIPGIGPTRKRA
                     LLQHFGSAKAVSRAGVADLRQVPGISAQMAQTIYDFFHERSV"
     gene            8149..8712
                     /locus_tag="VF226_00785"
     CDS             8149..8712
                     /locus_tag="VF226_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072554.1"
                     /translation="MRLTTIAAILAASLALSGPVLADEIEDSIKEALTAYEKKDFSAA
                     KHALDYASQLIAQKNAEGLGAVLPEPLSGWSAEDVETQDASVAMFGGIRAGRNYARGD
                     ERVSLQILGDSPLLSTWMPMMANPAMAAAMGKMTKVGKYRAIQTKDGQVIVVVANRFL
                     VMVDGSASIKDKVDYAAAIDFEKLESL"
     gene            complement(8793..9848)
                     /locus_tag="VF226_00790"
     CDS             complement(8793..9848)
                     /locus_tag="VF226_00790"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX7072555.1"
                     /translation="MTETPSHAEREVERARADLSDTLDALKEKLTFGQIFDEARTKFM
                     SGDGGDFLRNLWRQARDNPMPAVLAGTSLLWMMMGSNRERRQYGAATGFRTQGGDGLR
                     TAAESARKTGAGAMEQARHAAASVGESMRSMAGTVGETMSSAGHAARSAGETAGEFMQ
                     SAMSTAQDATRTAQQMGHETRRTISDLMEQQPLLIGAAGIALGALIGAMLPRTRLEDE
                     YLGEARDQLGEAISEQTGQLYERGKVTATEVYRAAAEEARAQGLVPEEGEGKTLAEKA
                     EQVLTKAGEKAKEVGQREMGTGEMGSGQAGSSGAGTSLPGTPQQTAAGQTSGVQPPEN
                     IRPGTPVMPDRTPSRGG"
     gene            complement(9845..10333)
                     /locus_tag="VF226_00795"
     CDS             complement(9845..10333)
                     /locus_tag="VF226_00795"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7072556.1"
                     /translation="MAEVRHTEADTPQSQPRVSEAGLGEPAAARTSTTSAKGNHSVPE
                     LIADLGRDITALFGKEAGLLRAEVNEKFANLQSAGGSLAAAGICLLVSLLILLQALIV
                     WLTNMGLGAGWASLLVGAVVGIIGIVLLQYGRSLMSQGIIPTRTQTQVQRDAEMLKEQ
                     VK"
     gene            complement(10339..10953)
                     /locus_tag="VF226_00800"
     CDS             complement(10339..10953)
                     /locus_tag="VF226_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072557.1"
                     /translation="MANDPNRSGLGPETEEAKRTIQREAESAQQTAQQLSENVQREAK
                     EVSSDIQAQAQQMVSEQKEKARSGLMDVVSAVRRAADDLEHHQQPQVANIARSLASGL
                     EDFSQAIGRRNLQDMLSDVENFARNHPTTFFGGSLLVGLALARFAKSSSRPSQSAART
                     EHIRQPGATYAAHTAPQTGQTGQYSEYGRHEREQSRGVEASITR"
     gene            complement(11225..12220)
                     /gene="meaB"
                     /locus_tag="VF226_00805"
     CDS             complement(11225..12220)
                     /gene="meaB"
                     /locus_tag="VF226_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX7072558.1"
                     /translation="MTQDASHEERTAPARADGTARDLAARVQAGERRAIARALTEAER
                     QSRLSSDLLRELQSDLGRALVIGITGPPGAGKSTLVDACLRELRKQGRTAGVIAIDPS
                     SPVSGGAILGDRIRMSVGLADEGVFVRSLAARGHLGGLSPAAVRMIDVLDAAGFERVI
                     IETVGTGQSEIDIAEVTDIRVVVGAPGLGDEIQAMKSGLLEIADILVVNKADREGASH
                     TVHQLKAALAMRAGGRSATPVLKTTALTGEGVPDLVRAIDEIGAKRLAIGPRARRRMR
                     ARYLIARAAAELVAAEVRQAETERIDELSDSVLEGRLSATEAAGQLIAGKVSRSE"
     gene            complement(12227..13201)
                     /locus_tag="VF226_00810"
     CDS             complement(12227..13201)
                     /locus_tag="VF226_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX7072559.1"
                     /translation="MYYLLDENTRRAHKLRNRLHSLLLLIGIGGVLGVSAILIWGWAG
                     LVWALLAVGILVVLAPRVPPEAVMRMYQAREVDLRFAGRVGRVIEILAERAGLAKTPR
                     LYVVPSSTLNAFATGSAENASIAVTAGLLQTLSLRELAGVLAHEMSHIRNNDLWLMGL
                     ADAMSRFTQALSTLAVMLAAFNVMGMLVGVEFISWWPILVLYLAPLVSSLLQLGLSRA
                     REYDADLEGVLLTGDPEGLISALAKLERYTGRFWEDLMFPVPGRRVPQPSILRSHPRT
                     EDRIARLRELAPQPKRPPIVVVDEPFDMASPWGDMLRRPRVRWPGVWY"
     gene            complement(13312..13938)
                     /locus_tag="VF226_00815"
     CDS             complement(13312..13938)
                     /locus_tag="VF226_00815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX7072560.1"
                     /translation="MQKLVSRGPMGGVRNICVYCGSGVGSKPAYAESARTLGKSLAQA
                     GIGLVYGGGSLGLMGEVARATLQAGGRVTGIIPAFLSEREQMLRDVDELVVTEDMHQR
                     KMLMFQKSDAFVALPGGIGTLEELVEQLTWSQLGQHQKPVIIANIEGFWTPFLRLLEH
                     MRSDQFIRQSLDVRCTVVDRAEDIVPAVLKAAPGEPRETGDKVLISRM"
     gene            14417..>15799
                     /locus_tag="VF226_00820"
     CDS             14417..>15799
                     /locus_tag="VF226_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7072561.1"
                     /translation="MTIQGSIKVAGSRRHAADVRGQEGRADHWTVLRGLLPFVWPKGR
                     PDLRLRVVLAFAVLILAKLVTVAVPVFFKQATDLLASSSGEAGLSLGQGVGLGAAALI
                     LAYGTGRVLMLVLNQIRDVFFTKVGQHAVRELNNRTFQHLHRLSLRFHLERRTGGLSR
                     VVERATRAIELIIRMGILNVVPTALELLLICGILLYYFDWIYVAIVLATVAAYMWFTF
                     AASEWRISIRREMNESDTEANTKAIDSLLNFETVKYFGNEELEARRFDASMARYESAA
                     IRTYYSLGVLNSGQSLIFTVGMTIVMLLTARGILAGENSIGDFVLVNSILIQLYMPLN
                     FMGMVYREIKQGLVDLENMFSLLDEAPEVVDKPGAEPLRVERGTIRFENVSFAYDPER
                     PILKDVSFEVPAGRMVAIVGPSGAGKSTISRILFRFYDISAGRVTIDGQDIRDVTQKS
                     LRAAIGVVPQD"
BASE COUNT         2714 a         5063 c         5157 g         2865 t
ORIGIN      
        1 cgtcacccca tccggggaga ttagcagcca ctgatcagca ttcaaagcgt cttggtgtga
       61 cggctctcca gcatcgggcc gatctggacc aggatggtgg cggagaacat cagcgcggcg
      121 ccgacccagc cgatgggcgc cagccattcg ccggcgaaca agacgcctgc cgccgcggcg
      181 aacagcgttt ccatgctcat caggatggag gcttcgcccg ggggcgtgtc tttgagcgcg
      241 atcgccagca gcgtgaaggt gagggcactg gacaggacac cgacataggc gacggccggc
      301 agggccttgt acaacccatc cagggtgttg tgctcgatcg ccagtgtcag gataccgccg
      361 ccgacagcga cacaggcgaa ctgcatggcc gtgtaggcga cgggcctgcc gtagatggtc
      421 gcgcggctgg taatcagcat gtggattgcc caagccaccg cagacgcggc aatcagcccg
      481 tcgccggtcg agaagcctcc gaccgttccg ccaccgagca gccagactcc gaggaaagcc
      541 aaggcgacgg ccggccagac gtagacggcc ggccgctgct tgaacgcaat ccaagccatc
      601 agcggcgtcg caacgacata gaggcccgtc agaaagcccg cgttcgtcac ggtggcatcg
      661 atgagcccgg tctgctgaag gctcgcaccg acgaggaacg cgaggccacc cgcgacgccg
      721 atctggacga ggccgctcga ccaggagttc gcgacgcgtc gcgcctcgat cagggcgagc
      781 ggcgtcaggc agacggccgc aatgaggcca cgcgcggcaa tgaacaggaa cgggccgata
      841 tgctccatgg ccgccttctg aaaatagaat gcaacacccc agacggcggc ggcgagaagc
      901 agcaggagat cggctctgag gcggcgcatg agggcgaggt gatagttggc cgctgaacgc
      961 ggcgcaatga aattaagtcg cagggcagag ccgtgccgtc gcataacgtc gcgctggaca
     1021 agtcctgcgc cgcaccgtac tggcttgcgg cttctccgtc aggatgtcac tgtccatgcc
     1081 cgccgccggc tctttgtcgt ggccacacat tgcgccgctc gtcctgctcc ttgcctcgaa
     1141 cgtgttcatg accatcgctt ggtacgggca tctgaagttc aagactgcgc cgctgctcgt
     1201 cgtgatcgtc ggctcatggg cgatcgcctt cgtggaatac tgcctcgcgg tgccggcgaa
     1261 ccggatcggg catgccgtct attcggccgc cgagctgaaa acgatgcagg aggtcatcac
     1321 cctcgtgatt tttgccgcct tttccgtgct ctacttgggt gagccgctga cctggcggca
     1381 tgcagcgggt ttcgcgctga tcgcgctcgg cgccggtttc gtcttttcgg cgcgggcctg
     1441 aaggtgtgcc cgagccggta atcctgctcg tattgatggc agcggcgttc gcaggggctt
     1501 tcgtccaggc ggcggtgggc tttggctttg cgctgatcgc tgccccgatc ttcctcacgg
     1561 ccatgaaatc ggggtcggcg atgcaggtgc tggtcgccat ccatgtcgtg cagagcgcga
     1621 tgcttgttcc cgggctctgg cgctcggccc cggcgaagct gcttcatccc atgctgctcg
     1681 ggagcctcgc cgggttcccg ctggggctcg cggtgttcgt cgcgctcgac ctcgacgcgc
     1741 tgaagctggc ggtcgggatg gtgatgctgg tcttcacggc gttgctggtc gcgcgcgacc
     1801 ggagctggct cagccagatg ttcgcctcga gcgggccgcc gaaccgggcg gcagcggcag
     1861 gcgtcggcgc atccgccggg gcgctgacct cagttctggt gatgcccggg ccgccgctca
     1921 tcctctatgt cgccgcgcgc cggatcggga aggaggagag cagggcgctg tcgctgacct
     1981 tcttcgcgtt ctgctacgtg gccgttaccc tgctccacgc cacgtggacg ggtatgagct
     2041 ttgagacgtg ggtcttggcg gcggcgctgt ccccggtggt ggtgatcggg acgctgcttg
     2101 gggacaggct ggcgcgctgg ctcagcgagg cctggttccg caccgccgtg ctggcggtct
     2161 cattcctctc cggagcgtgg gccgtctgga gctcgttttg agcgaaatgc ccgggaccac
     2221 agccggggtt ccccgatctc gaccgacggt ggcaaagttc gcgcccaaaa cccctctccc
     2281 cggcggggag tgggttaggc tgagggggga ccgacgctcg ctgatgttct aatccctcac
     2341 ccggcccttc gggccgacct ccccccatgg gagaggtgag gcgagtgcgc gcagcatcgc
     2401 ttccaacttg gtcggaaacg ctctacagcc cgcgaacgag attgccgacc atcagcacca
     2461 tgtaggccgt gatcgccagc cagtattcct ggtggggaag gtagcggggc acgtgcagga
     2521 agccgagctt cgtcgccacg gcgagcagtg ccagcacgag cgagatcaga aacaccgcga
     2581 tggtcggcgg attgagacgc atggcaaagc cccctccgag gcgtcagccc acctctttac
     2641 agcgtgcgat gcgtctcgcc aatgcccatc gggctgagtt cgtcggcctc gatgatgaag
     2701 cgaacgaggc gaggatggct cgtcccggtc tcccgcgtgg tcaccagcag gaagcgcggt
     2761 cctgccttgc tctccacccg ggtgaggttc gggccgatct cgcgaatatc gatgacttcg
     2821 agcgtcctgt tgccgttgcc cccggatatc tcgatgcgat ctccaacctg tacgggcttc
     2881 gacaatccca gtgggagctc atcgtccagg cggctcagcc agaaattggg gctctctgcc
     2941 aaggcctgct tacgttgagt gttcgggggt gtatcaggag cttgcgccac cggggattca
     3001 gcgagggccg cctcgacacc ccggtcgagg atcgcgtcgg ggcggaaaac cgtccatgca
     3061 agcacgccca cggcggtaca agcgctcgcc agcgcgatga gcggtaagcc tgcaatgatc
     3121 ttgcgaccgt tgaccatgtt ccgtctccaa gctccgtcgc tcaagtcgaa tatataggac
     3181 gaccggcgac gcgtgcgaac cctgctgccg cctgcgattc tgtcccagct gtaccacgta
     3241 tttctggtcg gcctcttggc cggcaatgtg gccgcgggcg gtgccgcgcc ggtctggtgt
     3301 tgttcagatt gccgatagag cgggctctga tttgctggat tcgccctctt tcgcgggtat
     3361 tgtagccggg cagccacata acgacgcagg aggacgccca tgaagctgac acccgagcag
     3421 ctcaagcagt tcgaggacga gggctggttt ttcctccccg attgcttttc ggaggaagag
     3481 gtctcggtac tccgtcggga ggccgagcag atcttcagcc agagccgcca ggaaatctgg
     3541 cgcgagaaat ccggcgcacc ccgcacggcc tttgctgcgc acacctacaa cgaggcattc
     3601 ggcatcctcg cccgacaccc gcgtctcgtc gagccggtcg agcaggtgtt cggcgagaag
     3661 ctctacatgc accagttcaa ggtgaacgcg aaggccaagt tcgacggcga cgtctggcaa
     3721 tggcatcagg attacggcac ctgggcgcgt gatgacggca tgcccgagcc gcgcgccatg
     3781 aacatctccg tgttcctgga cgaagtgttc accttcaacg ggccgctgat gctgatcccg
     3841 agaagccaca agcacggcgt tctcgaagcc gggcacgaca aggcgacgac gagctacccg
     3901 ctttggacgc tggaccacga gaccgtggag cgcctctgca acgacggcgg ccttgttgtg
     3961 ccgacgggca agccggggag cgtgctcatg ttccacggca acctggtgca cggctccgct
     4021 ggaaacatca cgccattccc gcgcaagatc gtgtacctga cgctttgtgc cgtctcgaac
     4081 tacatccgga agccgacgcg gcccgagtgg atcgcgcacc gcgacttcac gccgatcgag
     4141 acgtgcgcgg acgacgcttt gctgacgtat gcacggcagt accggcaggc ggcggagtag
     4201 gccgttgtat cacggcctgg cttccgacac cccggacagc gtgagccttg gcgagcgctg
     4261 atccggggtg gatggtttgc ccggaatgcc tacagggtgg gcagcagcag gaacatcgct
     4321 gcggcgacca gtgtcggccc cactgcggcg gccagcaggc ccagcggctt gatggcgccg
     4381 tcgtcgaagc tgcctttcag gattgcgaag ccggccgggt tgggcgcatt ggcgatgacg
     4441 gtgaggccgc cgccggttac cgcgccagcg acaagcgcgt acttgaagtc gtccgtgacg
     4501 ccttcgacca gcgacccgag gtaggtgagt gccgcgttgt cggtgatggc cgtcagggcc
     4561 gtggcgcccc agtagagtac cgcaggtgac atgccttcca gggtcggctg caaccaccac
     4621 ttctgcagtc cgccgagcac gacgaggccg gcgaggaaga aggccaccat cagcccctca
     4681 cggatgagca gccgggactg atactgcttg taggcctccg tgaagccgag gaagaacagg
     4741 aagatgccgg caaacacgac cagatggtga ttgaacacca ccacgcccgt gagaaacatc
     4801 aggtgcacgg ccacgaccca gcttggcaca tcaaggcgcg cgagggctgc cgacatcgtc
     4861 tgaacgctct tcaggtggtt gcggaagaga taggtcacca caaccgcatt gatgaacacc
     4921 gcaatggccg ccttccagcc gaagtgcaac agcatgaagg ccatgtccca gccccaggtg
     4981 ccggcgacca tcaggacggg cggcgccgcg aatggcgtca gaacaccgcc gatagaaaca
     5041 ttgacgaaca agaccccgat ggtcgcgtat ttgaacacgt tgggcgcgtt cctgttgatg
     5101 aaccgctcgc gcagcagcag cgccgaaagc gtcatcgcgg ccggctccgt gatgaacgag
     5161 ccgaggaggg ggccgatcgc cagcacggta aggtagtagg cggccgacgc tggaagtggc
     5221 aggattcgcg agatccagac caggagcgcg ccagcgaagt cgatgatggg ccggctggcg
     5281 gcgatcacca tgatgacgaa gacgaaggcc ggctcggtaa aattgcggct ttcgaggtag
     5341 tgcatcgacg agtcggtgcc gagctgaatc gcaaccagca ccaggagcac catggcccag
     5401 aagccgaaca ctgcttccac ttcaccgagc aggtgccaca ggcccgcgtg cgcgggttga
     5461 accttggcca gatgctcgaa gtaggctgtc gagaaggtgt gaatgacagc caacgcaaac
     5521 agtgctgcag ccagaatttc gacggagtgt ggaaggatca tttcggccat gcgtggtccc
     5581 cgtcagattg gcgcatgctc ggaagctgct ggggcagtcg agtgggtggg tgcgccaccg
     5641 tgcatcaacg gggccatgag ttcaacagcc aattggaccc agcagccccg gatacaacgc
     5701 ctcggcctgc tgcgggtggc gctccgctcc cgggctccag cgacgggttt caggtatcgc
     5761 gcgcaggtta cacgctgcgt tcgtggaaga agtcgtagat cgtctgtgcc atttgcgcgg
     5821 atatgcccgg cacctgcctg agatcggcca cgcctgcgcg ggaaacggct ttagccgatc
     5881 cgaagtgttg cagcagagca cgcttgcggg tggggccaat accggggatc tcgtccagcg
     5941 ggttggcgga gatcgccttc gcgcgtctcg cgcggtgagt cccgatcgcg aatcggtgcg
     6001 cttcgtccct gagccgctga acgaagtaga ggaccggatc gcgcggctcc agcatgaacg
     6061 gcttttcccg gccgggcagg tagaagtgct cgcggccggc atcgcggtcc gggcccttgg
     6121 cgacacccac gatggggatg tcctcgatcc cgagctcgcg gaggacatcc gcggccactc
     6181 caagttgccc cgcgccgcca tcgatcagca cgagatccgg gcggtcaggg aactcttcct
     6241 ccgctggttc cggttcgcgc tctgcgggcg tctcgtcgaa aagagccggg gagcagtcgg
     6301 gcggctccgg gcggttctcc ccatgatttt cgagccccgg ctcggagacg gtttccgccc
     6361 gcagcatacc gaatggaatc ccgtcagcgc ctctgcgggt gaccggatcc ggagcaggcg
     6421 gttcgatccg cgtcgcgggt tcgccgtccg gaggcgagat gctgtggccc gacaccggtt
     6481 ggggctcacg ggcggcgggc cgttctcccg cgtgcttgtc cgcgagtcgc ttgaaccggc
     6541 gcgtcagcac ctcgcgcatc atggcatagt cgtcacccgg cgtgaggtcg gcggacttga
     6601 tgttgaactt gcggtactgg ctcttcacga acccgtccgg cccggcgacg atcatggcgc
     6661 cgaccgcatt ggttcccatg atgtggctgt tgtcgaagac ctcgatccgc cggggtgtgg
     6721 cagggagccc gaaccgctct gcgatgcctt cgagcaggat gcgctgtgac gagctctcgg
     6781 caagtctcct cgccagcgcc tcgcgggcgt tccgcaacgc atgctcgacg acaccgagct
     6841 tgctgccgcg ctgcggcgcc cggacctcca cccggcggtc ggccttgctc gacagcgctt
     6901 cggccagcaa cgcgcggccg ggaagatttt cggacagcaa aagcagccgc ggcaccggtt
     6961 tgtcgtcgta gaactgggcg atgaagctct ccagcacttc agccgtgccg agcgaccgat
     7021 ccgccttcgg aaaataggcc cggttgcccc agttctggcc gctgcggatg aagaagacct
     7081 gaacgcaggt ctggccgcca tcctgagcga tggcgaatac gtcagcttct tcgacgccct
     7141 cgggattgat cgcctggtcg gcggtgacgt gggcgagggc ccagaggcgg ttgcgcagct
     7201 ccgccgcgcg ctcgaactcg agggcttcgg ccgcctcctc catcagcttc tggtaatggc
     7261 ggcgaacgtt ctggctttcg cccctcagga accgggtcgc ctcgtcgacg agggcgttgt
     7321 aatcctcgag gctgatctcg ccggtgcagg gcgccgagca gcgcttgatc tggtagagaa
     7381 ggcacggccg cgtgcgggtc tcgtagacgg cgtccgagca ggaccgcaac aggaacgccc
     7441 gctgcagcat ggtaattgta cgattaacag cgcctgccga agcgaacggg ccgaaatact
     7501 cgccgcgccg cgtccgggct ccgcggtgct tcaggatctg cggcgcgcga tggtcgcggg
     7561 caatgagaat gtacggaaac gacttgtcgt cccgcatcag cacgttgaag cgcgggcgga
     7621 agcgcttgat gagattggct tcgagcagca gcgcctcggc ctcggtgccg acgaggacga
     7681 actccatcgc ggccgtctgc gagatcatgc gggcaatgcg ggtgctgtgg ccggcgaagc
     7741 gcgtgtaatt ggcgacgcgc gccttcaggc tccgcgcctt gccgacatag atcacgtcgc
     7801 ccttggcatc aatcatgcga tagacaccgg gcgagttcgg caaattcttc agatatgagg
     7861 cgatgacctc gggaccggtt ttcggagagg tgcgctcctg aaccgccgcc tcccgcgcct
     7921 cgctcacgcg atcctgtggc ataccgaaga cttaggcctg gatggccgaa tcgtccagag
     7981 aacgctggtc gcagctaagg aagctgcggg atttcaggcg gaaagcatcg gtcgctgtga
     8041 tgcaggatca cgacagctcg tgatttccac ggctaacgcc cgcgcgcccc gttgccccct
     8101 ctgtgaaggg ctctaccgtc catggctcga ttcacctgga gactaggaat gcgactcact
     8161 acgattgctg ccatcctcgc cgccagcctg gcattatccg gacccgtcct tgcggacgag
     8221 atcgaggact cgatcaagga agcgctgacc gcctacgaga agaaggactt ctccgcagcc
     8281 aagcacgcgc tcgactacgc gtcccagctc attgctcaga agaatgcgga aggccttggc
     8341 gccgtgctgc ccgaaccgct gagcggctgg tcggccgagg acgtcgaaac ccaagacgcc
     8401 agcgtagcaa tgttcggcgg catccgcgcc ggacgcaact acgcgagggg tgatgagcgc
     8461 gtctcgctgc agatcctcgg cgactcaccg ttgctgtcca cctggatgcc gatgatggca
     8521 aacccggcca tggccgccgc aatgggcaag atgaccaagg ttggcaagta ccgcgccatt
     8581 cagaccaagg acggccaggt tattgtcgtc gtggcgaacc gtttcctggt gatggtggac
     8641 gggtcggcct cgatcaagga caaggtggac tacgccgcgg ccatcgactt cgagaagctg
     8701 gagtccctct gagcacttgc cacccaggcg cgaaaaaggc tgctcaccca aatggatgag
     8761 cagcctgcct gaaatcgacc gcggcaggca gatcagcctc cgcggcttgg tgtgcggtca
     8821 ggcatgacgg gcgtgcccgg cctaatgttt tcaggtggct ggacgccgct tgtctgtccg
     8881 gcagccgtct gctggggggt gcccggtaag ctggtaccgg cacctgaaga ccccgcctgg
     8941 ccggatccca tctcgccggt acccatctcg cgctggccga cttccttggc cttttctcca
     9001 gctttggtga gcacctgctc ggccttttcg gcgagcgtct tgccctcacc ttcctcgggg
     9061 acgagccctt gcgcgcgagc ctcttcggca gcggcgcgat agacctccgt ggcggtgact
     9121 ttgccgcgct cgtaaagctg gccggtctgt tcggaaatgg cttcgccgag ctgatctctc
     9181 gcttcgccga gatactcgtc ctcgaggcgg gtccgaggca gcatcgcgcc gatcagggcg
     9241 ccgagcgcaa ttccagcagc tccgatcagg aggggctgct gctccatgag atcgctgatc
     9301 gtgcgtctcg tttcatgtcc catctgttgg gcggttctgg tggcgtcctg ggcagtgctc
     9361 attgccgatt gcatgaattc tcctgcggtc tcgcccgccg agcgggcagc atgccccgcc
     9421 gagctcatgg tttcaccgac ggtgccggcc atggagcgca tcgactcacc gacgctggcg
     9481 gcggcatggc gggcctgctc catggcgccg gcgcccgttt tgcgcgccga ttcggctgcc
     9541 gtccggaggc cgtcgcctcc ttgtgtgcgg aagcccgtcg ccgcgccgta ctggcgccgc
     9601 tcacggttgc tgcccatcat catccagagg aggctcgtgc ccgccagcac ggcaggcatc
     9661 ggattgtccc tggcctgccg ccagagattg cggaggaagt cgcccccgtc accgctcatg
     9721 aatttggtgc gcgcctcgtc gaaaatctgg ccgaaggtaa gcttctcctt cagggcgtcc
     9781 aacgtgtcgc tgagatcggc gcgggcgcgc tcgacttccc gttcagcatg gctcggagtt
     9841 tccgtcactt cacttgctcc ttcaacattt cggcatcgcg ttggacctgc gtctgggtac
     9901 gcgtggggat gattccttgg ctcatcagcg agcgcccgta ctgcagcagg acgataccga
     9961 tgatgcctac gacggctccg accagcagcg atgcccagcc cgcgccaagg cccatgttcg
    10021 tgagccagac gatgagcgcc tgaagcagga tgagcagcga gacgagcagg cagatgccag
    10081 cggccgcaag gctgcctccc gcggactgga ggtttgcaaa cttctcgttg acctcggcac
    10141 gcagcaggcc ggcttccttg ccgaagagag cggtgatgtc gcgacccaga tcggcgatca
    10201 gctcaggcac tgaatggttg cccttggcgc ttgtggttga agtccgcgcc gctgcgggct
    10261 cacccaatcc ggcctcggag accctgggtt gggattgagg agtgtcggct tccgtgtgtc
    10321 tgacctctgc catggcgttc acctcgtaat gctggcctcg acgccccggc tctgctcccg
    10381 ctcgtggcgg ccgtattcgc tgtattgtcc ggtctgcccg gtctgcggcg ccgtgtgcgc
    10441 cgcgtaggtc gcgccgggct gcctgatgtg ctcagtgcgc gccgctgact ggctgggtcg
    10501 gctcgagctc ttggcaaacc gcgccagtgc gagcccgacg agcaacgagc cgccaaaaaa
    10561 cgtggtcggg tggttccgcg cgaaattttc cacgtccgaa agcatgtcct gcaggttccg
    10621 ccggccgatc gcctgcgaga aatcctcgag cccactggcg aggcttctgg cgatgttggc
    10681 gacctgcggt tgctgatgat gctccaggtc gtcggcagct cgccgcacgg cggaaacgac
    10741 atccatcaga cccgatcttg ctttctcctt ctgttcggag accatctgtt gggcctgcgc
    10801 ctggatgtcc gaagacacct ccttggcttc gcgttgaaca ttctcgctga gttgctgggc
    10861 ggtctgctgc gccgactccg cctcgcgctg aattgtgcgc ttggcttcct cggtttccgg
    10921 ccccaggccg gatcgattgg ggtcgttcgc catcgggccg ttactccact gcttgatcca
    10981 tcggcgcgcc ggaaaggaaa cacaattcgc cagcggcaag atcgtcagga ccaccaccgc
    11041 aggtccaacc gagtaaaagg gaaaatttgc gctcgcccgg cggggtgatt tgatcttcca
    11101 gcgcttcaat tcaagtaaca gacggccaga ctattggttc cgcttctgga aaatcggcct
    11161 ctgctgcaat ctaattgact ggcaattagc ttgaactgaa gcagaagggg cgccgaagcc
    11221 ggtatcactc ggatcgcgat actttccccg caatgagctg accggcagcc tcggttgcgc
    11281 tcagtctgcc ttcgaggacg gagtcggaca gctcatcgat ccgctccgtt tccgcctggc
    11341 gcacctctgc tgccaccagt tcggcggctg cacgcgcgat cagatagcgt gcccgcatcc
    11401 gcctgcgcgc ccgtggaccg atcgccaggc gcttggcgcc gatctcgtcg atcgcgcgga
    11461 cgagatccgg caccccttcg ccggtcaggg ccgtggtctt gagcaccggc gtcgccgagc
    11521 gcccgcctgc acgcatggcc agcgcggcct tgagctggtg aacggtgtgg ctcgctcctt
    11581 cgcggtcagc cttgttgacg accaggatgt ccgcgatctc gagcaggccg gacttcatgg
    11641 cctggatctc gtcgccgagc ccgggtgcgc caaccacgac gcggatgtcc gtcacctcgg
    11701 cgatgtcgat ctcggattgt ccggtgccca cggtttcgat gatcacacgc tcgaaccccg
    11761 cggcgtccag cacgtcgatc atgcgcacgg cagccggcga gagcccgccc agatgaccgc
    11821 gtgctgcaag cgagcgcacg aagacgccct cgtccgcgag gccgacgctc atgcgtatgc
    11881 ggtcccccag gatggcgccg cctgacacgg ggctcgacgg gtcgatggcg atcaccccgg
    11941 cggtccgccc ttgcttgcgc aactcgcgca ggcatgcatc gaccagcgtc gacttgccgg
    12001 ctcccggcgg gccggtgatg ccgatcacga gtgcgcggcc gaggtcgctt tgcagctcgc
    12061 gcaagagatc agaggacagg cgggattgcc gctccgcttc cgtcagcgcg cgggcaatcg
    12121 cgcgccgctc tccggcctgg actcttgccg caaggtcacg cgccgtgccg tccgcacgcg
    12181 ccggcgcagt ccgttcctca tgcgaagcgt cttgcgtcat ggccggtcag taccacacgc
    12241 ctggccaacg gacgcgtggc ctcctcaaca tgtcgcccca aggtgaggcc atgtcgaatg
    12301 gctcgtccac caccacgatg ggcgggcgct ttggctgtgg cgcgagctcg cgcaggcgcg
    12361 caatgcggtc ctccgtacgt ggatgggagc gcaggatcga cggctgaggc acgcggcggc
    12421 ccgggaccgg gaacatgagg tcctcccaga agcggccggt gtatcgctcg agcttggcga
    12481 gcgccgagat caggccttcg gggtcgccgg tcagcaagac cccttcgagg tccgcatcgt
    12541 actcccgggc gcgcgacagg ccgagctgca ggaggctcga gacgagcggc gcgaggtaga
    12601 gcacgaggat cggccaccag gatatgaact cgacgccgac cagcatgccc atgacgttga
    12661 atgcggcgag catgacggcc agggtcgaca gcgcttgcgt gaaccggctc atcgcatccg
    12721 ccaggcccat gagccagaga tcgttgttgc ggatgtggct catctcgtgg gcgagaacgc
    12781 cggcgagctc gcgcaggctc aacgtctgga gaagacctgc ggtcacggcg atgctggcgt
    12841 tctccgcgct gccggtcgcg aaggcattga gcgtcgagct tggcacgacg tagagccgcg
    12901 gtgtcttggc gaggcccgcg cgctcggcga ggatctcgat gacgcggccc acacggccgg
    12961 caaaccgcag gtcgacctcg cgggcctgat acatccgcat gaccgcctca gggggcacac
    13021 gcggcgcaag cacgacgagg atccccacgg ccagcagggc ccagacgagg ccggcccagc
    13081 cccagatcag aatggccgag acacccagga cgccgccgat gccgatcaac agcagcagcg
    13141 agtgcaggcg gttgcgcagc ttgtgcgcgc gccgcgtgtt ctcgtcgagc aggtaataca
    13201 aaagctaggt gtccttgcgt gccgctctga ttcgatctcc ctcgagccta gttcattgtg
    13261 cgcgcgatgg catgggcgaa tactcgaccc atgctcaggt acaattcccg gctacatgcg
    13321 ggaaatgagg accttgtcgc ccgtctcccg cggctcgccc ggcgccgctt tcagcaccgc
    13381 aggcacgata tcctccgccc ggtccacgac cgtgcatctg acgtcgaggc tctgccggat
    13441 gaactgatcc gatcgcatgt gctccagcaa gcgcagaaac ggtgtccaga acccttcgat
    13501 attggcgatg atgaccggct tctggtgttg accgagctgc gaccatgtga gctgctcgac
    13561 cagctcctca agcgtgccga tgccgcctgg cagggccacg aatgcatccg atttctggaa
    13621 catcagcatc ttgcgctggt gcatgtcctc cgtgacgacc agctcgtcga cgtcccgcag
    13681 catctgctca cgctcggaga ggaaggcggg aatgatgccg gtcacgcgtc cgccggcttg
    13741 gagcgtcgcg cgggcgacct cgcccatgag acccaggctg ccgcctccat aaacgagacc
    13801 gatcccagcc tgtgcaagtg atttgccaag tgtacgggcc gactctgcgt acgcgggctt
    13861 gctgccgaca ccagaaccac aatagacaca aatattccgg actccgccca tagggccccg
    13921 gctgactagt ttctgcattt ttactgcctg cttgttcttc gtttcctcgc cgtagcgcct
    13981 ccacgacacc ttactacata tcgttctccc tgcttcgatt ggtaagccca gacccggacc
    14041 gaattgcggc gtgcctgccc ggttccggag caaagttttc cccccggtcg caatcgattc
    14101 cacgatggca tgctggacgg cgatcacacg acagtttgcc gggctgatgc acccgggcta
    14161 tgacaacccc ggcgagcccc tgccggttcc ttcaaatgct gatgatcgaa tctcgccgaa
    14221 gccgaaggtg gcgcgggatt cgccaagtct cgccggcgcg catatatggc ttggagagca
    14281 tcacagcggc tcggctgcag gtgcggcggc cctgacaggt gcgccatccg cgcggtgcgg
    14341 aaattcggga actttctcgg actttgggtt aggagtgcgg cacgagactg cggactcggg
    14401 tcaaacagga cgaagaatga cgattcaggg ttcgatcaag gtggccgggt cgcgcaggca
    14461 tgctgccgat gtacgggggc aggaaggccg tgcggatcac tggaccgtgc tgcgcgggct
    14521 gttgcccttc gtctggccca aggggcggcc ggacctgaga ctgagggtcg tgctggcctt
    14581 cgcggtgctg atcctcgcga agctcgtcac ggtcgccgtt ccggtcttct tcaagcaagc
    14641 caccgacctg ctcgcttcga gcagcggcga ggccgggtta agcttgggcc agggtgtcgg
    14701 tctcggagct gccgctctca ttctggccta tgggaccggt cgcgtcctga tgctggtgct
    14761 gaaccagatc cgcgacgtgt tcttcaccaa agttgggcaa cacgcagtgc gtgagctcaa
    14821 caaccgcacc ttccagcatc ttcatcggct gtccttgcgc tttcatctgg agcggcgaac
    14881 gggcggcttg agccgcgtcg tcgagcgggc gacgcgcgcc atcgagctca tcatccgcat
    14941 gggcatcctg aacgtggtgc cgacggcgct cgagctgctg ctgatctgcg ggattctgct
    15001 ctactacttc gactggatct atgtcgccat cgtgctggcg acggtcgccg cctacatgtg
    15061 gttcacgttc gcggccagcg aatggcggat ctcgatccgt cgtgagatga acgagagcga
    15121 taccgaggcc aataccaagg ccatcgacag cctgctcaat ttcgagacgg tcaaatactt
    15181 cggcaacgag gagctcgagg cgcgccggtt cgatgcctcg atggcccgct acgagagcgc
    15241 ggcgatcaga acgtactact cgctcggcgt gctcaactcc ggccagtcgc tgatcttcac
    15301 agtgggcatg acaatcgtca tgctgctcac ggcccgcggc atcctcgccg gcgagaactc
    15361 catcggcgac ttcgtgctgg tcaactcgat cctcatccag ctctatatgc cgctgaactt
    15421 catgggtatg gtctaccgcg agatcaagca ggggctcgtc gatctggaga acatgttttc
    15481 gctgctcgac gaggccccgg aggtggtcga caagcccggt gccgagccgc tgcgggtcga
    15541 gcgcggcacg atccgtttcg aaaacgtttc gttcgcctat gatcccgagc gaccgatcct
    15601 gaaggacgtc tcgttcgagg ttcccgcggg gcgcatggtg gccatcgtcg gcccctccgg
    15661 agcgggcaag tccaccatca gccgcatcct gttccggttc tatgacatct cggccggacg
    15721 ggtgacgatc gatggccagg acatccgcga cgtgacgcaa aagagcctgc gcgccgccat
    15781 cggcgtggtg ccgcaggat
//
LOCUS       DASVDU010000011        34790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1326759, whole genome shotgun sequence.
ACCESSION   DASVDU010000011 DASVDU010000000
VERSION     DASVDU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 34790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34790
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326759"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..177)
                     /locus_tag="VF226_00825"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="VF226_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008281741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(303..378)
                     /locus_tag="VF226_00830"
     tRNA            complement(303..378)
                     /locus_tag="VF226_00830"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(343..345),aa:Ala,seq:ggc)
     gene            complement(539..922)
                     /locus_tag="VF226_00835"
     CDS             complement(539..922)
                     /locus_tag="VF226_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX7072562.1"
                     /translation="MATKEARGTKRTCQNPECGSRFYDLNRDPIVCPICHTVYKLATA
                     PQGVSPQEEEAIRKPTKPEIAADEEVIAGDLPLAEGDEDLADLEDSDEAIVGGDDEAF
                     IEPEEEDGGDVSDILGPVGEDDEER"
     gene            1123..2445
                     /gene="aroA"
                     /locus_tag="VF226_00840"
     CDS             1123..2445
                     /gene="aroA"
                     /locus_tag="VF226_00840"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7072563.1"
                     /translation="MTASALNPLSASSAGPLRGRCRVPGDKSISHRALIFGALATGRT
                     RITGLLEAEDVINTAKAVAALGAPARKVGSVWEVLGRGVGGLSQPSGPLDFGNSGTGT
                     RLMMGVVAGHPITVAFVGDASLSRRPMSRVLKPLKQMGLEVLEDKDTLPLTIRGTSDL
                     VPIVYELPVPSAQVKSAILIAGVAAAGETTVIEREPTRDHTERMLRYFGADVRSVADG
                     ALTRITVKGHAELQGRDVVVPGDPSSAAFIAAAALIVPGSEVTIEGVLVNPTRTGFYT
                     TVREMGGDVAFLNEREEGGEPVADIRVRSSGLTGVTVPPERAPSMIDEYPILAVVAAF
                     ARGRTEMHGLAELKVKECDRLAATAAGLAVNGVAARVDGDTLIVEGAGEVRGGGTVAT
                     HLDHRIAMSFLTLGLASSAPVTVDDATMIATSFPEFQGLMASLGARFG"
     gene            2556..3182
                     /gene="cmk"
                     /locus_tag="VF226_00845"
     CDS             2556..3182
                     /gene="cmk"
                     /locus_tag="VF226_00845"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7072564.1"
                     /translation="MIIAIDGPAASGKGTLGKRIAEHFGLAHLDTGLLYRAVARCVLA
                     RGASLEDEAAAVECARNLDPESLSDPELREAGIGEAASIVARIEGVRAALLAFQREFA
                     RRKPGAVLDGRDIGTVVCPDADVKIFVTALPEVRAHRRYLERKARGDDISYGEVLDLI
                     RQRDERDASRASAPMRPAEDAILLDTTNLDIEAAFDAAVGLIKRKIGQ"
     gene            3368..5086
                     /gene="rpsA"
                     /locus_tag="VF226_00850"
     CDS             3368..5086
                     /gene="rpsA"
                     /locus_tag="VF226_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX7072565.1"
                     /translation="MSTPQTSNEMTPSREEFAAMLNETLAEDVSYEGNVVRGRVVSIE
                     KDMAVIDVGLKMEGRVPLKEFAAPGKPAELNVGDEVEVYLERMENALGEAVLSREKAR
                     REESWNRLEQQFERGEKVTGVIFNKVKGGFTVDLDGAVAFLPGSQVDIRPVRDIGPLM
                     HQPLQFQILKMDRRRGNIVVSRRSVLEETRAEQRADIVARLEEGQVIDGLVKNITDYG
                     AFIDLGGIDGLLHVTDMAWRRVNHPSEIVNVGDTVKVQIIRINPETQRISLGIKQLQA
                     DPWEGIEAKYPVGARFTGTVTNIADYGAFVELEPGVEGLIHVSEMSWTKKNVHPGKIV
                     STSQQVEVQILEVDPQKRRISLGLKQTQENPWEAFLAQHPKGSIVEGPIRNITEFGLF
                     IGLEGGVDGMVHLSDLDWNRPGDEVIKEYKKGDVVKAVVLDVDPSKERISLGIKQVAG
                     DPLDVISRFKKGDPVTCEVVAVQDNGIEVKIAGTDVTSFIKRSDLSRDRAEQRPERFN
                     VGDKVDAAVVSVDKASRRISVSIKQLELAEEKQAVAQYGSTDSGASLGDIFKAAIKKR
                     EDETKE"
     gene            5244..6200
                     /gene="sppA"
                     /locus_tag="VF226_00855"
     CDS             5244..6200
                     /gene="sppA"
                     /locus_tag="VF226_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7072566.1"
                     /translation="MSLDTESILDRRRVRRQLSFWRVLAVVALTFAVGAFFLSGDRGL
                     IDRRQIARVSIEGMITENRDFLNLLDRIGKDKRVAGVIVYVNSPGGTTTGGESIYEAL
                     RRLAEKKPVVAQFGTVAASAAYIAGLGTDYIVARGNSITGSVGVIMQWPEVSGLLDKL
                     GIRVNEVKSGPLKANPSPFQPLDESGRAIAEEMIAESHRWFLDLVSTRRQVTTSTIPG
                     LEQGRVFSGREALEYKLIDQIGGEPEAIRWLESQGVEKDLKVVNWKESSGVEWPLGGA
                     VAGVVRYLTGAIGGDLAGLVSAERLGILGLDGLVSVWQPSEN"
     gene            6239..6526
                     /gene="ihfB"
                     /locus_tag="VF226_00860"
     CDS             6239..6526
                     /gene="ihfB"
                     /locus_tag="VF226_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX7072567.1"
                     /translation="MIKSELIQKIAAANPHLYHRDVERIVNVIFDEIVNALARGDRVE
                     LRGFGAFTVKYRAPRLGRNPRTGAPVPVEEKFVPFFKTGKELRERLNRPAS"
     gene            6571..6948
                     /locus_tag="VF226_00865"
     CDS             6571..6948
                     /locus_tag="VF226_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072568.1"
                     /translation="MIGRILGLLFGFIAAVFLVTLAVANRHAVRLVLDPFDPLDPVLA
                     LELPFYAYIFAMLIVGVVMGGLATWISQSRWRRLARERGRDAARWRAEAERLTRERDA
                     YVAAQRRSPAQAADTRQLALASH"
     gene            6960..7919
                     /locus_tag="VF226_00870"
     CDS             6960..7919
                     /locus_tag="VF226_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX7072569.1"
                     /translation="MRFIDAAAVGKALSYPELVDVLDEAFRQGAISPPRHHHQVKLEG
                     RPEATLLLMPAWTTSAPGAETAGRYIGIKQVTVFPDNSRLGKPAIYGAYLLLSTETGE
                     PVALIDAPALTVWRTAAASALAARYLARDDASRMLMVGAGALAPYLVRAHASVRPIRE
                     VMLWNRSRDKAEALKRNLDGTGLAVTVADDLESAARRADLVSTATISSAPLIKGAWLK
                     PGCHVDCVGAFRPDMRETDDDVVRRARVWVDTMAGGLTEAGDIVMPLKAGVIQEADIQ
                     GDLYGLARGTAPRRASPDEITMFKSVGASIEDLAAAIAVYESR"
     gene            8064..8759
                     /locus_tag="VF226_00875"
     CDS             8064..8759
                     /locus_tag="VF226_00875"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7072570.1"
                     /translation="MTVKVKICGLKTEAALETALDAGADYVGLVFYPPSPRSLSPPEA
                     GRLAGMARGRAEIVALVVDADDALLDEIMRAVGPDLIQLHGKETPDRVKAIRERCGRP
                     VMKAIKVETRADAEAALAYRGAADLFLFDAKAPRDLANALPGGNGIAFDWHALDGVKG
                     KVRFMLSGGLDPENVTAAIRLTTPEAVDVSSGVEVRPGEKDLQLIRRFLAAAKAAQTY
                     EALAPAEVRSERA"
     gene            8756..10027
                     /gene="trpB"
                     /locus_tag="VF226_00880"
     CDS             8756..10027
                     /gene="trpB"
                     /locus_tag="VF226_00880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7072571.1"
                     /translation="MSGDIKTAAVSGPNSFRMGPDDHGFFGIFGGRFVAETLMPLILD
                     LERAYAEAKNDPEFQAELKALNAHYTGRPSPLYHAERLTAHLREVAARNGQKGGAKIY
                     FKRDELNHTGSHKINNCLGQILLARRMGKTRIIAETGAGQHGVAVATVCARYGLPCEI
                     FMGATDVERQKPNVFRMKLLGAKVNPVTAGAGTLKDAMNEALRDWVTNVRNTYYIIGT
                     AAGPHPYPAMVRDFQSVIGQEVREQMMEAEGRLPDTLVACIGGGSNAIGLFHPFLDDK
                     NVKIFGVEAGGYGLDVPDGHCASMTGGKPGVLHGNRTYLLQNEDGQILEGHSISAGLD
                     YPGVGPEHAWLKDTGRVTYVPVTDREALEAFQLCTRLEGIIPALEPSHALAYVCKLAP
                     TLPQNDLLVMNMCGRGDKDIFAVAEHLGMKM"
     gene            10053..10904
                     /gene="trpA"
                     /locus_tag="VF226_00885"
     CDS             10053..10904
                     /gene="trpA"
                     /locus_tag="VF226_00885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7072572.1"
                     /translation="MTAETRIDRRFASLKREGRGALVTFITAGDPDYDTCAQLLAGLP
                     AAGADVIELGMPFSDPMADGPAIQAASQRALAAGQNMHKTLALVSEFRKKDQDTPIVL
                     MGYYNPIYVYPTEAFLDDAKVAGVDGLIVVDVPPEADDEICLPAVERGLSFIRLATPT
                     TDSKRLPAVLANSSGFVYYVSITGITGTAAPEVEAVHAQVGRIRQATPLPVVVGFGVK
                     TPEQARAIAAGADGVVVGSALVNAIEQSLGPDGKATGKTVPAVLDLVKSLAGGLRTVS
                     SRPTMAG"
     gene            10986..12083
                     /gene="accD"
                     /locus_tag="VF226_00890"
     CDS             10986..12083
                     /gene="accD"
                     /locus_tag="VF226_00890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7072573.1"
                     /translation="MNWINNVVRPKIRSFLTTKREVPDNLWVKCPESGQMVFYKDLEA
                     NQFVVPGSNYHFRMAADVRLKHLFDGSEYTKYPVASVPLDPLKFRDGRRYTDRLKDAK
                     AETDLDDAVLVGEGTLDGQEVVAAAQDFRFMGGSLGMAAGEAVIAGMMRAVEKKTPFI
                     LFAASGGARMQEGILSLMQMPRTTIAVQRLREARLPYIVVLTDPTTGGVTASYAMLGD
                     IHIAEPGALICFAGPRVIQQTIREQLPEGFQRAEYLLAHGMIDMVVHRHQMRETLSRI
                     CRLLMANKAVIGKEKPKGLNGHGYMNGMPVDGKIIDPRSKSDSEDVIEAEPVADDERF
                     DPSKLEKREKGNRDGETGAARSRPEDGVHSS"
     gene            12149..13480
                     /locus_tag="VF226_00895"
     CDS             12149..13480
                     /locus_tag="VF226_00895"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEX7072574.1"
                     /translation="MPTSDEILTEMKRLHPQLIDLSLGRLERLLAKLGNPHDRLPPVI
                     HVAGTNGKGSTAAFLKAMLEAAGRRVHVYTSPHLVRFHERITLAGSDGRTRPIGETEL
                     VELLTRVSKVNAGEAITFFEITTAAAFLAFAETPADAVILEVGLGGRLDATNVISHPA
                     LSIITPVAMDHADKLGRTLAEIAGEKAGILKRGVPAVISQQEDEALQIICEQASRIGS
                     QLTVWGYHFDAYEQRGRLIFQQENVLLDLPKPALIGRHQIVNAGTAVAAAQALRRFGL
                     GEHAISRGLTEANWPARMQRLKDGPLSELLGSSSELWLDGGHNPAAGIVIAQTLADLE
                     ERSPSPLYLIVGMMGQKDASGFLSPFRGLVKEIVTVPIPGAHEAPHQPEALAEIARNV
                     GFRAAAASDVPSAIKRIEANDRMPKRILICGSLYLAGHVLAMQQGVTQQSN"
     gene            13718..14236
                     /locus_tag="VF226_00900"
     CDS             13718..14236
                     /locus_tag="VF226_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072575.1"
                     /translation="MSARKASRQRAPDWVAIRRLYEESTIPVAEIAELNGVTYSAIRN
                     RRLAENWKPRSKKSRTKVAPARRGRTTGRSGLVSRLLKVVDQNLKLLEMRMETDEPGT
                     AADRERETRAIGALTRTIEKLTELQSEAVAAPAKPAKTSSGTEIDETDADRLRLEIAE
                     RILRIGERYASR"
     gene            14157..15527
                     /locus_tag="VF226_00905"
     CDS             14157..15527
                     /locus_tag="VF226_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX7072576.1"
                     /translation="MRPTRTASALKLRNAFFALASGTHRDELAAALGELSSFELSFLA
                     WDWQTWARDDQLPPEADAQGRPWRTWLILGGRGSGKTRAGAEWVRAEVGLLARGSGSG
                     SGRIALVGETLDQVRSVMVEGVSGLLAVHPPDARPRFEQSKRQIVWPNGVVAQLFSAE
                     DPESLRGPQFAAAWLDEIAKWRYAEQAWDMLQFGLRLGANPRQVVTTTPRPVPILKRI
                     MGEDSTVITRVRTRDNAANLAPAFVAEMERRYAGTLLGRQELEGELIEDMSGALWRRE
                     WIEQHRLAVAPQLQRVVVAVDPPVTATSSSDACGICVAGLGPDGRAYVLADRTLQGRE
                     PQVWARAAIAAYRDFAADRVVAEVNQGGDLVRDILRQIDPAVPIRMVRATRGKWVRAE
                     PVAALYAEGRVAHVGHHSALEAQMLAFGIDGLAGGRSPDRLDALVWALTELILDSPPR
                     PAMRML"
     gene            15572..16783
                     /locus_tag="VF226_00910"
     CDS             15572..16783
                     /locus_tag="VF226_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX7072577.1"
                     /translation="MERQPSRLARMMRALSLRSPRGRERKAAAPGPLVAMEGLRTPAW
                     MPRDYGAFAREGMMQNPIVYRAVRMISEAAASVPLLLYEGEHELEEHPFLELLRRPGS
                     GRTTTDLMEAWYGYLLVAGNVYIQAIALWGRIQELHVLRPDRVRVIPGSDGWPEAFEY
                     HVSDRRVLIGGEVLPGIDRILHLKLFHPANDHYGLSPVEPAATAIDLHNTASRWNKAL
                     LDNSARPSGALVYSSGGHLTEEQFKRLKAELEANFQGARNAGRPLLLEGGLDWKAMSL
                     TPRDMDFIEARHAAAREIALALGVPPMLLGIPGDNTYANYQEANRTFWRQTVLPLVNR
                     TAKSLSAWLGPAWSDRLELRPDLDAIEALSSEREALWARVERASFLTRNEKRAAVGYG
                     PLPGGDELVSP"
     gene            16831..17562
                     /locus_tag="VF226_00915"
     CDS             16831..17562
                     /locus_tag="VF226_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX7072578.1"
                     /translation="METLETRPLMAAREVKLTSLGLKDVTLDGTFEGYASLFHREDLG
                     RDVVMPGAFRESIARRGAAGIRMLFQHDANQPIGTWKKIYEDARGLFVRGQLATEVGK
                     AREVLSLMRAGAIDGLSIGFRTVKGTRDSRTGVRRLEKIDLWEISIVTFPMLPEARVS
                     TVKSNPFGGRVPTEREFERWLTQDAGFTRSEARAVLRDGLKGLRLLRDAEQASSWEQR
                     LLAQMAEATRLLSQNMLMRGFTLSC"
     gene            17556..18797
                     /locus_tag="VF226_00920"
     CDS             17556..18797
                     /locus_tag="VF226_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX7072579.1"
                     /translation="MLNETSLEVKSATNADLSIAFEDFMRAFEAFKETNDRRLTEIER
                     HMSADVVTVDKLARIDRALDEYKGAIDELSRKAGRPMLGSAVRLSGGLDHKSAFEGYM
                     RRGESNLLRSFESKGLSVGSDSDGGYVVPEETERSINQAVRDISPIRAIAGVRQVSGS
                     VYKKPYAISGAEAGWVGETDERPETSSPKLAELAFPTMELYAMPAATQTLLDDAAVDI
                     DQWIAEEVRATFAAQEGTAFVTGDGVNKPKGFLSYPTVDNASWTWGNIGTIATGVSGA
                     FPDTDPADKLIDLIYSVKGEYRANAHFVMNRSTQSAVRKLKDADGSYLWQPSARPGEA
                     ASLMGVPVAESEDMPDIAADSHAIAFGDFRRGYLIVDRVGIRVLRDPYSAKPYVLFYT
                     TKRVGGGVQDFAAIKLLKFGA"
     gene            18963..19538
                     /locus_tag="VF226_00925"
     CDS             18963..19538
                     /locus_tag="VF226_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HEX7072580.1"
                     /translation="MGLVLTAAPAVEPITVEDAKMHLRIDHGDEDMLLASLIAASRLQ
                     VEAALDAALITQSWSLELDDWPDGRELHLPIWPVQSVEAVRISDRDGEMVELGAEAFS
                     LDGASKPPRLVSISGDWPGPGTRALGIEILFTAGFGNSANDVPPPIRQALLMLVAHWY
                     EHREPVETGKPTASIPEAISALLMPYRTVRI"
     gene            20006..20416
                     /locus_tag="VF226_00930"
     CDS             20006..20416
                     /locus_tag="VF226_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEX7072581.1"
                     /translation="MTSSSLALQIAIFEALRTDAGVLAALGGPRVYDHVPRKTDYPYL
                     TFAQTTVQDWSTGADEGDEHIITLHVWSRVESRTQLQEIIAAVRAALHDRDLPLIGHR
                     LINLRYQSAETRREPDGERFRGIIRLRAVTEPLA"
     gene            20452..20865
                     /locus_tag="VF226_00935"
     CDS             20452..20865
                     /locus_tag="VF226_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEX7072582.1"
                     /translation="MAAQKGKDLLLKIDSDGLGAFETVAGLRSRSLSFNTEVVDITHQ
                     ESTGQWRELLAGAGAKSARIAGSGIFKDAASDSAIRQAFFDGLIRTWRVVIPDFGTVE
                     GPFQITSFELTGRHDGEVAFDIALESAGELSFSAL"
     gene            20877..21257
                     /locus_tag="VF226_00940"
     CDS             20877..21257
                     /locus_tag="VF226_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gene transfer agent family protein"
                     /protein_id="HEX7072583.1"
                     /translation="MANARRGEIEACLNGKTWKLCLTLGALAELEQAFGDDDMLALAQ
                     RFEKGRLSATEAVRILGAGLRGAGHDVSDEEVKAMRTENGIVGIVDIVARLLAATFGM
                     PGGAAEESMPRSEVRSLDPFRGTT"
     gene            21257..21460
                     /locus_tag="VF226_00945"
     CDS             21257..21460
                     /locus_tag="VF226_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEX7072584.1"
                     /translation="MAAGLGLLRLPPAAFWAMTPKELAAALGALHGPARIDPPSRTEL
                     RRLMQAYPDEHQPGRIESEIHNG"
     gene            21453..22031
                     /locus_tag="VF226_00950"
     CDS             21453..22031
                     /locus_tag="VF226_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEX7072585.1"
                     /translation="MAEPVETWTVAIDADTSRLQQELANATRIGRQFGNALTNAFQGV
                     ALRGRDLGEVLKSLTLSLSRMALQAAFRPLEQGLSSLIGGAFSGVAFAKGGVVQNALP
                     VPFASGGVIASPVTFPLAGGRIGIAGERGPEAIMPLTRGPDGKLGVRAQGSAGVSVTI
                     NVSTPDVEGFRRSESQIAAMLARAVAMGQRNL"
     gene            22070..22708
                     /locus_tag="VF226_00955"
     CDS             22070..22708
                     /locus_tag="VF226_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HEX7072586.1"
                     /translation="MAFHEIRFPTDISRGAVGGPERRTEVVVLGSGHEERNSRWADSR
                     RSYNAGYGIKALDDLYAVISFFEERRGRLYGFRWRDHSDWKSCPPSQVPAATDQLIGT
                     GDGTTAIFQLTKTYGSAHAPWRREVKKPVAGSVIVAVDGVIQTEDVAFTVDSTSGLVT
                     FQPGHIPQPGSKVTAGFLFDVPVRFDTDKLEINLQGFRHGAIPNIPIVEIRL"
     gene            22705..23574
                     /locus_tag="VF226_00960"
     CDS             22705..23574
                     /locus_tag="VF226_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HEX7072587.1"
                     /translation="MKSLPPGLQAHLDSGATTLCWCWRLTRRDGVQIGFTDHDRDLTF
                     DGTTFEAAAGFTATEMKHSVGLGVDDLEVEAALTSERLSQDDLAAGLYDDARVEIFRT
                     NWSDPEQRVLMRVGSLGEVSRSGASFRAEVRGLSHYLQQPNGRLFQFTCDADLGDRRC
                     GVDLTSDSYRATGIVTAVSSPRAFEVSGLDEFTEGWFARGLLTFRSGANQGRAIEIRH
                     HRRRDGAALIELWQEPAKAVAIGDEVDVTAGCDKQLSTCREKFANVVNFRGFPHMPGN
                     DFLTSYARRGGGR"
     gene            23601..24023
                     /locus_tag="VF226_00965"
     CDS             23601..24023
                     /locus_tag="VF226_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7072588.1"
                     /translation="MAVVRIARSWIGTPYHHQASLIGCGTDCLGLVRGIWRELYGTEP
                     ATLPPYTRDWAEATGRETLLDAARRHLVEVENEKAQPGDVLVFRLRECLPAKHVAILA
                     TGTTMIHAMEGVSVAEVAFAPWWRRHLAGVFSFPGIED"
     gene            24025..27981
                     /locus_tag="VF226_00970"
     CDS             24025..27981
                     /locus_tag="VF226_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HEX7072589.1"
                     /translation="MATLALAAAGAAAGSALLPSGLTVLGATIGGATIGAQVGALAGS
                     FVDQALFGASGQSRTFTGPRISDLRVTASTEGAPIPRVYGRARVGGQVIWATDFEEEV
                     VTSQTGGGGKGGGSSGASKQVEYRYYGNFAVGLAEGEISGIGRVWADGQELDLSSVTW
                     RLYTGSEDQEPDSLISAHEGAESTPAYRGLAYVVFERMPLASYGNRLPQLSFEVFRAV
                     DDFHRNVRGVVLIPGSGEFVYATEEVTRLEAGGVQLAENVHTRQGGTDWTVSIDQLQS
                     SLPNARSVSLISSWFGTDLRASHCEIRPGVEIAKKSTKPLTWSVAGLSRSAAHVVSLH
                     EGRPAYGGTPSDQTVISAIRDLKKRGFSVVLTPFILMDVPAGNSKPDPYKGGAPGQPA
                     YPWRGRITVELAPGQPGTTDKTVAAAAEVAAFVGTASVSDFAVSGDRVAYGGQPEWSY
                     RRFILHHAFLAKAAGGVDAFVIGTEMRGLTQIRSGPQDYPFVAALVQLAADVKQVLGP
                     ETKVTYAADWSEYFGHQPQDGSNDVYFNLDPLWASPDIDAIGIDLYWPLSDWRDGADH
                     LDVLAGARSAYDLDYLKSNIAGGEGYEWYYASAADRNEQRRTPITDGQGKPWVFRYKD
                     LKSWWLNQHFNRPGGAEQATPTEWVPQSKPIWFMEIGCPAIDKGSNQPNVFVDPKSAE
                     TALPYFSNGKRDDFIQRRYLQALIEAFDPEHPGALPALNPISDIYGGPMVDPSRMHVY
                     AWDARPFPAFPADTETWGDGANWQRGHWITGRIASAPVADLVRRILDDSGFHAHDASA
                     LTGILPGYTIDRLMAPREAIQPLELAYFFDAVETGERIVFRPRGAGRQVIEVSTDDLV
                     EERTGANLVTITRGQETELPGSAKITYVSAANDYRQAVVEARRLAGASARVSQADLAL
                     VLEDEQAAAIADAWLFEAWAARERALFVLPPSKMAIEPSDIIAISEGGRSRLFRVKEI
                     GDHGARQAEALSIDPALYFLGEGPARRHRISPPTASGQPLGLLLDLPLLRGDEPEHAA
                     YFAASQSPWPGGVALFRSPTETGYTLQGVAEVPAITGELLDALPPGPVGRFDKAARLR
                     VQLAVGELASAEELQLLAGANAAAVRNAAGDWEVLQFQSAVLVGPKTYELSGFLRGQA
                     GTEGAMESEIPPGAPFVLITQALVRVDLAPADVGLEYNWRFGPANRDLGHPSYVQTRH
                     AFRGVGLRPLSPVHLRGRRSLNGDLALAWIRRTRVGGDSWETAEVPLAETVERYEVDI
                     LDGASVKRTISTTEPATIYTADQQMADFGVLPSALNVRVYQMSSVWGRGSPCSAVV"
     gene            28030..29325
                     /locus_tag="VF226_00975"
     CDS             28030..29325
                     /locus_tag="VF226_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02594 family protein"
                     /protein_id="HEX7072590.1"
                     /translation="MEQPAWLEHAWREAGVREEPGSASNDRILQFFRDVGHQTVTSDE
                     VAWCAAFVGACLERSGFRSTRSLLARSYLEWGEPLAKARIGAIAVLSRGSDPGQGHVG
                     FVIGATAEELFLLGGNQQNAVSVQSFEIDRLLGFRWPAGAVEKSSATDELFDLALAHV
                     LEMEGGWTNDPHDPGGPTNLGITLGVYAASKGTTITDANREDLIGELKRLDAASARPI
                     YYERYWVPSRAAELPPPIALMHFDAAVNHGVGNAARMLQQALGVAVDGEIGPETLGAA
                     RTHPVMETVHRYADIRRARYRSLSHFWRFGRGWLRRVDRTLAAAERLGDAATKASRQL
                     QKGAGQMSATEGAAADSKWWGHSMTIWGAIITAASTVLPAIGPLLGIDITPDLVNEFG
                     DQVTNVLQAIGGLIGIIMTIYGRTRASTGLGRKQITLQL"
     gene            29567..30238
                     /locus_tag="VF226_00980"
     CDS             29567..30238
                     /locus_tag="VF226_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7072591.1"
                     /translation="MRALVVEDDPDLNRQLKQALADAGFAVDSASDGEEGYFLGETEP
                     YDVIILDIGLPKMDGISILEQWRRAGRNMPVIILTARDRWSDKVAGIDAGADDYLAKP
                     FHMEELLARVRAQVRRSAGHAKSELECGPLRLDTRTARVTCDGQPIKLTSHEYRLLAY
                     LMHHNERVVSRTELVEHLYDQDFDRDSNTIEVFIGRLRKKIPADLIKTVRGLGYRLTQ
                     EPDEA"
     gene            30228..31610
                     /locus_tag="VF226_00985"
     CDS             30228..31610
                     /locus_tag="VF226_00985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7072592.1"
                     /translation="MRLNSLAFRLFATAIVSTMLILPVAGLLIYSVYRQEAEEGFDER
                     LRQLLTIVHADSIDESSSEPGTPGQVGEPLFFITHSGWYWQIKPLNGAPGQTKASPSL
                     ANFVLPSPFEEGIEPDVQGTRWLDVLGPNNQPVRIAETIYAFGEDSSGPQYSIAVAGP
                     LDWLEARVASFGTQLAFALTLAGIALVAVTLLQVRFGLLPLRAIENGLAAVRSGKSTR
                     LEGELPAEIEPLQHELNALIQSNEEVIERARTQVGNLAHALKTPLAVITNEAREDKSP
                     LAQKVAEQAQVMRDQVNHYLDRARMAASAGSLVRVTDVLPVAEALQRALERIYHEKGI
                     AIRLDCPEDARFQGERHDLEEMLGNLLDNACKWGRRSIRVSISVRRPELRGASPRLIV
                     TIEDDGPGLTEDQRRRIGKRGLRLDETKPGSGLGLSIVSDLALSYRGRLELGASRYGG
                     LLAKLDLPAA"
     gene            complement(31624..32532)
                     /gene="hemF"
                     /locus_tag="VF226_00990"
     CDS             complement(31624..32532)
                     /gene="hemF"
                     /locus_tag="VF226_00990"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX7072593.1"
                     /translation="MLPQPDLLDQWRDRASRWFGALRDEIRSAFERLEDELPPGSPLS
                     ELPPGRFIERAWSRTDHTGAPGGGGLMSIMKGRVFEKVGVHTSTVFGEFAPEFRSQIP
                     GASEDPRFWASGISLIAHMQNPHVPAVHMNTRMVVTTKWWFGGGADLTPLLETRRTQS
                     DPDTIAFHEAMRMACTPHPVADYERFKEWCDEYFYLPHRKEMRGVGGIFYDYLTPPEA
                     DGGWEAAFAFTQDVGRAFRNIYPELVRRNWHKPWTEAEREEQLVRRGRYVEFNLLYDR
                     GTVFGLKTGGNVDSILSSMPPVAKWP"
     gene            32704..34545
                     /locus_tag="VF226_00995"
     CDS             32704..34545
                     /locus_tag="VF226_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072594.1"
                     /translation="MYRWFETRIDAFPQEVPQQPPNRLPAFYWHFIRPVWPVFVLLLV
                     VGLIGSLIEVALYAFIGDIVDRMKEAPSPETFIADNGLLLLLMAFVALILRPLVSTAH
                     DLTKNQMIAGPVNTRVRWQSHRYVLRQSLGFFQNDFAGRVAAKVLQTGHALRESVVQI
                     IDSLWFAVVHWGAAIVLFADADGRLVIPLVIWLAAYGVTIWYFVPRIQRLSFNASEAR
                     SVLTGRVVDSYTNILTVKLFAHAEREDESARWALQNHLDRYLESQRNLTCMEFVLYSI
                     NGALMVSVTGLAIWLWSTGVVTIGAVALATALVIRLVGMSGWILTTVASVFESIGTVE
                     EGMETISRPVDVVDRPDAKPLVVKRGEIRFEDVSFHYGKHGGVIDRISLRIAPGEKVG
                     LVGRSGAGKSTLVNLLLRFYDLEGGRILIDGQDIACVTQDSLRAQIGMVTQDTSLLHR
                     SVMENILYGRPDAGEAAAIAAARKAHAHDFIVGLRDARGRTGYAAQVGERGVKLSGGQ
                     RQRVAIARVLLKNAPILILDEATSALDSEVESAIQEQLYNLMEGKTVIAIAHRLSTIA
                     AMDRLVIMDEGRIIEQGSHGELLARGGLYAELWARQSGGFLAKEAAE"
     gene            complement(34599..>34790)
                     /locus_tag="VF226_01000"
     CDS             complement(34599..>34790)
                     /locus_tag="VF226_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HEX7072595.1"
                     /translation="AFPGQEIEGKVTAMAPVLAGPHMTSRGPRRPDDAEVREVTIEFE
                     DAGPLIPGLRVDTFFRREP"
BASE COUNT         6348 a        10659 c        11443 g         6340 t
ORIGIN      
        1 ggcaagtttg aatgcagcca tgttgagcgt gaactgcgcg ctgatcgtct cgaggccgcg
       61 cagcattgtc ttggcgacct ggcctacggt cttgccccag ccgaagggct tctcgacctg
      121 tttgcgcttc tgctggctca cggcgtaacc cgggtggcgt gtcgtgcgcc catcgatgac
      181 tcgctcatca gcactcacgc gaactcattc ctgcgcacaa attttgtggg gtagtacgtg
      241 gggtgtggtt ttggagagaa gcctaaaacg caaggaaatg caactgttta gcaccatgct
      301 attggtggag ccgaggggaa tcgaacccct gacctcgtga atgccattca cgcgctctcc
      361 caactgagct acggccccga ccggttggga aattgtgggt gacggggagg caagcccccg
      421 cgacacgtgc gcctcaatag gctgacggga ggaaaactgc aagcccttcg ttcaggtccg
      481 cgacagggcg cgagcccaca cgcgagctgt gaaccgggcc agcggcgaag tcggcctctc
      541 agcgctcctc gtcgtcctct ccgaccggac cgaggatgtc cgaaacgtcg ccgccgtcct
      601 cctcctcggg ttcgatgaag gcttcatcgt cgccgccgac gatagcctca tcggaatcct
      661 cgagatcggc cagatcctca tcgccctcgg cgagcggcag atcaccagca atcacttcct
      721 cgtccgccgc gatttcgggc ttcgtgggct tgcggatggc ctcttcctcc tgcgggctga
      781 cgccctgcgg agccgtcgcc agcttgtaga cggtatggca gatggggcac acgatggggt
      841 cgcggttcag gtcgtagaaa cgggagccgc actccggatt ttggcaggtg cgcttcgttc
      901 cgcgcgcttc tttggtcgcc atctcgaatc ctcgatgtgt tgggtcatgg aaaaaatctg
      961 ctgccgtctg ccatgcacat caggtcgtgt caaaagcatt tgccagccat gccgacttgc
     1021 cgcggttgac agcttcgggc cggcaagctc tatctgccca tgcgcctgcc ggagcctggt
     1081 tccggcgctg ttccaaccac ctcgtgccgg ccggtcctgc cgatgacagc ttctgctcta
     1141 aatccacttt ctgcgtcatc ggcggggccg ctcagaggcc ggtgccgggt gccgggcgac
     1201 aagtccatct cccatcgcgc tctgatcttc ggcgccctgg cgacgggccg gacacggatc
     1261 acgggcctgc tcgaggcaga ggacgtcatc aatacggcaa aagcagtggc ggcccttggc
     1321 gcgccggcgc gaaaagtggg ctcggtctgg gaggtgctgg gccgcggcgt cggcggcctc
     1381 agccagccat ccgggcctct ggacttcggc aactcgggca ccggcacccg cctcatgatg
     1441 ggggttgtcg ccggccaccc gatcaccgtc gcgttcgtcg gagatgcctc gctttcgcgc
     1501 cgccccatga gccgggtgct caagccgctg aagcagatgg gtctcgaggt gctcgaggac
     1561 aaggacacgc tgcccctcac gatccgcggc acaagcgacc tcgttccgat cgtatacgag
     1621 ctgccggtgc cttcggcgca ggtcaaaagt gcgatcctca tcgccggggt tgcggccgcg
     1681 ggggagacga ccgtcatcga gcgcgagccg acccgcgacc acaccgagcg gatgctgcgc
     1741 tatttcgggg cggacgtccg ctccgttgcc gacggtgccc tgacgcggat caccgtcaag
     1801 gggcatgcgg agctccaggg ccgggatgtt gtcgtgcccg gagatccgag ctcggccgcc
     1861 ttcattgccg ccgcggcgct gatcgtgccc ggttccgagg tcacgatcga gggcgtgctg
     1921 gtcaacccga cccgaaccgg cttctacacc accgtcaggg aaatgggcgg agatgtcgcc
     1981 ttcctgaacg aacgcgagga gggcggcgag ccggtggccg acatccgcgt ccggtcatcg
     2041 ggcctgacgg gcgtcacggt cccgcccgag cgcgcgccca gcatgatcga cgagtacccg
     2101 atcctggccg tcgtcgctgc tttcgcgagg gggcggaccg agatgcatgg cctcgcggag
     2161 ctgaaggtca aggaatgcga ccggctggcc gctaccgcgg ccggcctggc agtgaacggc
     2221 gtcgctgcca gggtcgatgg cgatacgctc atcgttgagg gagccggtga ggtgcgcggc
     2281 ggcggcacgg tagcaacgca cctcgaccat cgtatcgcga tgtcgttcct gacactgggg
     2341 cttgcgagtt cggcgcccgt gacggtcgat gatgcaacaa tgatcgcgac cagctttccc
     2401 gagtttcagg ggctgatggc gtcactcggt gcgcggttcg gctgagccgg ccttgcgaga
     2461 gcgttagcca gagcgcggca atgacgaggg agcgcgatga tttattcgca gcccaggcag
     2521 cagggcggat ttccgcggat ggcgggttcg gcgccatgat cattgccatc gacgggccgg
     2581 ctgcatcggg caagggcacc ctcggcaaac gcatcgccga gcacttcggg ctggctcatc
     2641 tcgatacggg cctgctctac cgggcggtgg cgcgctgcgt gctggcccgc ggcgcgtcac
     2701 tggaggacga agccgcggct gtcgagtgcg cgcgcaatct cgatcccgaa agtctgtccg
     2761 accccgagtt gcgggaggca ggcatcgggg aggcggcatc gatcgtggcg cggatcgagg
     2821 gggtgcgtgc ggcgctgctc gcgtttcagc gcgagtttgc tcgccgcaag cctggcgccg
     2881 tcctcgacgg gagggacatc ggcacggtcg tctgcccgga tgccgacgtg aagatattcg
     2941 tgacggcgct gcccgaggtt cgcgctcacc gccgctacct ggaacgcaag gccaggggtg
     3001 acgacataag ctacggcgag gtactggatc tcatacggca gcgcgacgag cgtgatgcga
     3061 gccgcgccag cgcgccgatg cggcccgccg aagacgccat attgctcgac accactaact
     3121 tggatataga agcggcattc gacgccgccg tcggactgat caagaggaag atcggccaat
     3181 agggtgcgcc tcaggtgcgc ccctaatcct gttggcgggc agacccctct gccggtggcc
     3241 tcgctgaaat catcgattgg gaactcgcac gctggacgtt gccagccggg cattgtcatg
     3301 tgccggcagg gcgacatggg tgcgcacaac acggcgggct gcccgccacg gagctgaagg
     3361 gtacttgatg agcacacctc aaacctcgaa cgaaatgacg ccgtcccgcg aagaattcgc
     3421 ggccatgctg aacgagacgc tggcggaaga cgtcagctac gaaggtaacg tcgtacgcgg
     3481 cagggtcgtc tcgatcgaaa aggacatggc ggtgatcgat gtcggcctga agatggaagg
     3541 ccgcgtcccg ctgaaggagt tcgccgctcc cgggaagccg gcggagctga atgttggcga
     3601 cgaggtcgag gtctacctcg agcgcatgga gaatgcgctc ggcgaggcgg tgctgagccg
     3661 tgagaaggcc cgccgcgagg agagctggaa caggctcgag cagcagttcg agcgcggcga
     3721 gaaggtcacc ggcgtcatct tcaacaaggt caagggcggc ttcaccgtcg acctcgatgg
     3781 cgcggtggcg ttcctgcccg gcagccaggt cgatatccgg ccggtgcgcg acatcggccc
     3841 gctgatgcac cagccgctgc agttccagat cctcaagatg gaccgccgcc gcggcaacat
     3901 cgtcgtttcc cgccgctcgg tgctggagga gacgcgggcc gagcagcggg ccgacatcgt
     3961 ggcccggctc gaggagggcc aggtcatcga tggtctcgtc aagaacatca ccgactacgg
     4021 tgcgttcatc gatctcggcg gcatcgacgg cctgctgcac gtcacggaca tggcctggcg
     4081 ccgcgtcaac catccctccg agatcgtcaa tgtcggcgat accgtgaagg tccagatcat
     4141 tcgcatcaac ccagagacgc agcgcatcag cctcggcatc aaacagctcc aggccgatcc
     4201 gtgggagggc atcgaagcca agtacccggt cggcgcccgc ttcacgggca cggtgaccaa
     4261 tattgcggat tacggcgcct tcgtggagct tgagccgggc gtcgagggcc tgatccacgt
     4321 ctccgagatg agctggacca agaagaacgt ccaccctggc aagatcgtct cgacgagcca
     4381 gcaggtggag gtgcagatcc tcgaggtcga tccccagaag cgccgcatca gcctcggcct
     4441 gaagcagacg caggagaacc cgtgggaggc gttccttgcg cagcacccga aggggagcat
     4501 cgtcgagggg ccgatccgca acatcaccga attcggcctg ttcatcggcc tcgagggcgg
     4561 cgtggacggc atggtccacc tgtcggacct cgactggaac cggccgggcg acgaggtcat
     4621 caaggagtac aagaagggcg acgtggtcaa ggccgtggtg ctcgacgtcg acccatccaa
     4681 ggagcggatc tcacttggca tcaagcaggt cgccggtgat cctctcgatg tgatcagtcg
     4741 cttcaagaag ggggacccgg tcacctgcga ggtcgtcgca gtccaggaca acggcatcga
     4801 ggttaaaatc gcgggcaccg acgtgacgag cttcatcaag cgctcggacc tctcccgcga
     4861 ccgtgccgag cagcggcctg agcgcttcaa tgtgggcgac aaggtcgacg cggcggtggt
     4921 cagtgtcgac aaggcgagcc gccggatctc ggtgtcgatc aagcagctcg agctggccga
     4981 ggagaagcag gccgtcgcgc agtacggctc gaccgactcg ggcgcctcgc tcggcgacat
     5041 cttcaaggcc gcgatcaaga agcgcgagga cgagacgaag gagtgagcca ccacgcgagt
     5101 tgagatttcc gggagcgcag tcgttggctg cgctcctttc gtcttgggaa tggcgagtga
     5161 attattgaac gacccaccgg agcagcatac tttctacggc caaggtcttg tcgatagccg
     5221 cccgcatgtg gcacgggagc aacatgagcc tcgataccga gagcatcctc gatcgtcgcc
     5281 gggtgaggcg gcagctgtcg ttctggcggg tgctggccgt cgtcgcactg acgtttgcgg
     5341 tcggcgcgtt cttcctctcc ggcgatcgcg ggctaatcga tcggcgccag atcgcccgtg
     5401 tttccatcga aggcatgatc acggaaaacc gtgatttcct gaaccttctg gaccgcattg
     5461 gcaaggacaa gcgcgttgcg ggcgtgatcg tctacgtcaa cagtcctggg ggcaccacga
     5521 cgggcggcga atccatctac gaggccctgc ggcggctcgc ggaaaagaag ccggtggtcg
     5581 cccagttcgg gacagttgcc gcctcggccg cctacatcgc cgggctcggg accgattaca
     5641 tcgttgcccg gggcaactcg atcaccgggt cggtcggcgt catcatgcaa tggccggaag
     5701 tttccggtct gctggacaag ctcggcattc gggtgaacga ggtgaagagc gggccgttga
     5761 aggccaaccc ttcgccgttc cagccgctcg acgaatcggg gcgcgccatt gccgaggaga
     5821 tgatcgccga gagccaccgc tggttcctgg acctggtgag cacgcgccgg caggtcacga
     5881 cgagcaccat tcctggtctg gagcaggggc gggtcttctc tgggcgcgaa gccttggaat
     5941 acaaattgat agatcagatc ggcggcgagc cggaggccat tcgctggctc gaaagccagg
     6001 gcgtcgagaa ggatctgaag gtggtcaact ggaaagagag cagcggtgtc gagtggccgc
     6061 tcggcggcgc agtggcaggt gtggtccgct acctgaccgg cgcaatcggg ggtgaccttg
     6121 ccgggctggt ctcagcggag cggctgggga ttctcggcct tgacggcctc gtgtcggttt
     6181 ggcaaccttc cgaaaactga ttaaaataca atcactttgc cttcgtccgg agtccgtcgt
     6241 gatcaagtcc gaactcattc agaagattgc cgccgccaat ccgcacctgt atcaccggga
     6301 cgtcgagcgg atcgtcaacg tcatcttcga cgagatcgtc aacgccctgg cgcgcggcga
     6361 tcgcgtggag ctgaggggct tcggcgcgtt cacggtgaaa taccgggcgc cacggctggg
     6421 gcgcaatccg aggaccggag cgcccgtgcc ggtcgaggag aagttcgttc cgttcttcaa
     6481 gacgggcaag gagctgcgcg agcgcctcaa ccggccggcc tcgtgacggc aggcaggagt
     6541 ggccgggaca gttcgggaag ggcgcgcgtt gtgatcggtc gcattctggg tctgttgttc
     6601 ggcttcattg ccgccgtttt cctggtgacg ctggcggtgg ccaaccgcca tgcggtccgc
     6661 ttggtgctcg atccattcga tccgttggac ccggtcctgg cgctggagct tcctttttac
     6721 gcgtatattt tcgcgatgct catcgtcggg gtggtgatgg gtggcctcgc cacgtggatt
     6781 agccagagcc gctggcggcg attggcgaga gagcgtggtc gggacgcggc gcgctggcgg
     6841 gcggaggccg agcgcctcac ccgtgagcgc gatgcctatg tggcggcgca gcggagatcg
     6901 ccggcgcagg ccgccgatac gcgccagctc gcgcttgcga gccactgaag ggtccattca
     6961 tgcgcttcat cgatgcggcc gcggtcggca aggcgctgag ctatcccgag ctggtcgatg
     7021 tgctcgacga ggcatttcgg cagggagcca tatcgccgcc ccggcaccat catcaggtga
     7081 agctcgaggg ccggccggaa gccacgctgc tgctcatgcc ggcgtggacg acatccgcac
     7141 cgggcgcgga gacggctggc cgctacatcg gcatcaagca ggtcaccgtt ttccccgaca
     7201 attcccgcct cggcaaaccg gcgatctatg gggcctatct tctcctctcg accgagacgg
     7261 gcgagcccgt cgccctcatc gacgcgcccg cgctcaccgt ttggcggacg gctgctgcat
     7321 cggcgctggc ggcgcgctat ctcgcgcggg acgacgcgag ccgcatgctc atggtggggg
     7381 cgggcgcact agcgccttat ctcgttcgcg cccacgcctc cgtgcgtccg atccgcgagg
     7441 tgatgctctg gaaccggagc cgtgacaagg cggaggccct aaagcgcaac ctcgacggaa
     7501 cggggttggc cgtcacggtc gccgacgatc tcgagtcggc ggcacgtcgc gcggatctcg
     7561 tttcgacggc gaccatctcc tcggcgccgc tcatcaaagg cgcctggctg aagccaggat
     7621 gccatgtcga ctgcgtcggc gcgttccggc ccgacatgcg cgagacggac gacgacgtcg
     7681 tccgccgcgc ccgtgtctgg gtggatacca tggccggcgg acttaccgag gcaggcgaca
     7741 tcgtgatgcc gctcaaggca ggcgtgatcc aagaggcaga cattcagggc gatctctacg
     7801 ggctcgcgcg cgggacagcg ccgcgccgcg cctcgccgga cgagatcacc atgttcaagt
     7861 ccgtcggagc ctccatagag gatctggcag cggctatcgc cgtctacgag agccggtagg
     7921 tcgaagccag catttgcccc atgatggggc gcgcgctgcg gggatcggtc gagttgcctt
     7981 tcctcgccgc tcggtgcata gcaggagcct tccaggacat atcgggacgc gcactcggtt
     8041 attgcgtcca gactggcatc aggatgacgg taaaggtcaa aatttgcggg ctgaaaaccg
     8101 aggcggcgct cgagacagcg ctggatgccg gagccgatta tgtcggcctc gtcttttatc
     8161 cgccgagccc gcggagcctt tcgccgcccg aagcaggaag gcttgccgga atggcgcgtg
     8221 gccgtgccga gatcgtggcc ctggtggtgg acgcagatga cgccttgctc gacgaaatca
     8281 tgcgggcggt cggccctgac ctgatccaac tccacgggaa agaaacgccg gaccgtgtca
     8341 aggccatccg ggagcgttgc gggcgacccg tcatgaaggc gatcaaggtc gagacacgcg
     8401 ccgacgccga ggccgcactc gcctatcgcg gggcggccga tctgttcctg ttcgatgcga
     8461 aagccccgcg ggacctggcg aatgccttgc caggcggaaa cggcatcgcg ttcgactggc
     8521 atgcgttgga cggcgtcaag ggcaaggtcc ggttcatgct gtcgggcggg ctcgacccgg
     8581 agaacgtgac ggctgccatc aggctgacaa cgcctgaggc cgttgacgtc tcctccggag
     8641 tcgaggtgcg gcccggtgaa aaggatcttc aattgatcag gcgatttctg gcggcagcca
     8701 aggctgcgca aacttatgaa gcgttggcac ccgcagaggt gaggagcgag agggcatgag
     8761 tggcgatatc aaaacagctg ccgtttcggg gcccaacagc ttccgcatgg ggccggacga
     8821 tcacggcttc ttcgggatct tcggcggccg gttcgtcgcc gagacgctga tgccgctgat
     8881 cctcgatctc gagcgcgctt acgcggaagc aaagaacgac ccggagtttc aggccgagct
     8941 gaaagccctc aacgcgcatt atacgggacg tccatcgccg ctctatcatg ccgagcgcct
     9001 gacggcgcat ctgcgcgagg tggctgccag gaacgggcag aaaggcggcg cgaaaattta
     9061 cttcaagcgc gacgagctca accacaccgg cagccataag atcaacaact gcctcgggca
     9121 gatcctgctc gcgcggcgga tgggcaagac ccggatcatc gctgagacgg gggccggcca
     9181 gcacggtgtt gccgtcgcga ccgtttgtgc gcgctatggg ctgccgtgcg aaatcttcat
     9241 gggtgcgacc gatgtcgaga gacagaagcc caatgtcttc cgcatgaagc tgctgggggc
     9301 gaaggtcaat cccgtgaccg ccggtgcggg tacgctcaag gacgccatga acgaggcgct
     9361 gcgggattgg gtcaccaacg tccggaatac gtattacatc atcggcaccg ccgcgggccc
     9421 gcatccctat ccggcgatgg tgcgcgactt ccagtcggtg atcggccagg aggtgcgcga
     9481 gcagatgatg gaggccgaag gccggctgcc cgacacgctc gttgcctgca tcggcggtgg
     9541 atccaatgcc atcggcctgt tccatccgtt cctcgacgac aagaacgtaa agatcttcgg
     9601 cgtcgaggcc ggcggctacg gccttgatgt gcccgacggg cactgcgcct cgatgacggg
     9661 cggcaagccc ggcgtgctgc acggcaatcg gacctatctt ctgcagaacg aggacgggca
     9721 gattctggag ggccactcga tctcggccgg actcgactat ccgggcgtcg ggccagagca
     9781 cgcgtggctc aaggacaccg gcagggtgac ctacgtgccg gtaacggaca gggaggcgct
     9841 ggaggccttc cagctctgca cgcgactcga gggcatcatc ccggcattgg agccgtctca
     9901 cgcgctcgcg tacgtctgca agctcgcccc aacgctgccg caaaatgatc ttctggtcat
     9961 gaacatgtgt gggcggggcg acaaggacat cttcgccgtg gctgagcatc tcggcatgaa
    10021 gatgtgatgc aaaggggcgg ggatacttcg gaatgactgc tgaaacacgc attgaccgcc
    10081 gctttgcctc gctgaagcgt gagggacgag gtgcgctcgt cacgttcatc acggctggtg
    10141 atcccgatta cgacacctgt gcgcagcttc tcgctggtct gccggcagcc ggggcggacg
    10201 tcatagagct cgggatgccg ttttccgacc cgatggctga cggcccggcc atccaggctg
    10261 catcccagcg agctctggcg gcgggccaga acatgcacaa gaccctggcg ctcgtctcgg
    10321 aattccggaa gaaggaccag gacacgccca tcgtcctgat gggctattac aacccaatct
    10381 atgtctatcc tactgaggca ttcctggatg atgccaaggt tgccggcgtc gatgggctga
    10441 tcgttgtcga tgtcccgccc gaagccgacg atgagatctg cctgcccgcg gtggaacgcg
    10501 gcttgagctt tatccgcctc gccacgccga ccaccgattc caagcggctg cccgccgtgc
    10561 tcgccaattc gagcggcttc gtctattatg tttccataac cggaatcacc ggcacggcgg
    10621 cgcccgaggt cgaggcggtc cacgcacagg tcggcaggat cagacaagca acgccgcttc
    10681 cggtcgtcgt cggcttcggc gtcaagaccc ccgagcaggc cagagccatt gcagcaggtg
    10741 ccgacggagt ggtggtgggt tcggctttgg tcaacgctat cgagcaaagc ctcgggccgg
    10801 atggcaaggc gactggtaag accgtgcctg ccgtcctaga tctggtgaaa agcctggctg
    10861 gtggattgcg gactgtgagc agccggccca ccatggcggg ctgagatcgc tgaaatctac
    10921 gaggacgggg aagagatcgc tgagggcgta tcgtataggt ttcggggtgt gaggagcgct
    10981 cgagcgtgaa ctggatcaac aacgtcgtgc gtccgaagat ccgcagcttc ctgacgacga
    11041 agcgcgaggt cccggacaat ctttgggtga aatgtccgga aagcggccag atggtctttt
    11101 acaaggacct cgaggccaac cagttcgtcg tgcccggctc gaactatcat ttccgcatgg
    11161 cggcggatgt gcggctgaag cacctgttcg acggctccga atacaccaag tacccggtcg
    11221 ccagcgtccc tctcgaccct ttgaagttcc gcgacggccg gcgctatacg gaccggctga
    11281 aggacgccaa ggctgaaacc gacctcgacg atgcggtgct tgtcggtgaa ggcacgctcg
    11341 acggacaaga ggtcgtggcc gcagcgcaag actttcgctt catgggtgga tcgcttggca
    11401 tggccgctgg cgaggccgtc atcgccggca tgatgcgtgc ggtcgagaag aagacaccgt
    11461 tcatcctgtt tgcggcctcc ggcggtgcgc ggatgcagga gggcatcctg tccttgatgc
    11521 agatgccgcg tacgaccatc gcggtgcaac ggctgcgaga ggcgcgcctg ccctatatcg
    11581 tcgtgctgac agatccgacg acgggaggcg tgaccgcctc ctacgcgatg ctcggcgaca
    11641 tccatatcgc cgagccggga gctctgatct gctttgccgg cccccgcgtc attcagcaga
    11701 ccatccggga gcagctgcca gagggcttcc agcgggccga gtatctgctg gcgcacggca
    11761 tgatcgatat ggtggtgcat cgccatcaaa tgcgcgaaac gctctcccgg atatgccggc
    11821 tgctgatggc caacaaggcc gtgatcggga aagaaaagcc caagggcctc aatggtcacg
    11881 ggtacatgaa cggaatgccg gtggacggca aaatcatcga ccctcgaagc aagtccgaca
    11941 gtgaagacgt catcgaggcc gaaccggtcg cagatgacga gcgcttcgat ccgtcgaagc
    12001 tcgagaagcg cgagaagggc aaccgggatg gcgaaactgg tgccgcccgc tcccgtcccg
    12061 aggacggcgt ccacagttcc tgactgcgtt atcagctatt agtaattttc gccgctcggg
    12121 ccaggccgaa gcggtatgcg gagcattcat gccgacgagc gacgaaatcc ttaccgagat
    12181 gaagcggctg catccgcagc tcatcgacct gtcgctcggg cggctcgaac ggctgctggc
    12241 gaagctcggc aatccgcacg acagattgcc gccggtgatc catgtcgccg ggacgaacgg
    12301 caaaggctcc acggccgcat ttctaaaagc catgctggag gcggccggcc ggcgggtgca
    12361 cgtttataca tccccacact tggtgcgctt tcacgagcgg atcacgcttg ccgggtcgga
    12421 tggccggacg cgaccgatcg gcgaaacaga gctggtcgag ctgctgacgc gcgtgtccaa
    12481 ggtgaatgca ggcgaggcca tcaccttctt cgaaatcacg acggcagcgg cctttctggc
    12541 atttgccgaa acgcctgccg atgccgtcat tctggaagtc ggcctcgggg gccggctgga
    12601 cgcgacgaac gtcatctcgc accccgcctt gtcgattatc acgcctgtag ccatggatca
    12661 cgccgacaag ctcggcagga cgctggcaga aattgccggc gagaaagccg gtattctgaa
    12721 acggggcgtt ccggccgtca tctcgcagca ggaggacgag gcactgcaga tcatctgcga
    12781 gcaggcctca cgcattgggt cacaactgac cgtctggggt tatcattttg atgcctacga
    12841 gcagcggggg cgtttgatct ttcagcagga aaacgtgctc ctcgacctgc cgaagcctgc
    12901 attgatcggg cgacaccaga tcgtcaatgc gggaacggca gtcgcggccg cgcaagcgct
    12961 gcgccgcttc ggactgggtg agcacgcgat cagccgtgga ctgacggagg ccaattggcc
    13021 cgcgcgcatg cagcgtctga aggacggtcc gctgtccgaa ctgctcggat ccagttcgga
    13081 gctgtggctc gacggcgggc ataatcctgc cgccggaatt gtcattgcgc aaacgctcgc
    13141 ggatctcgag gagcgctcgc cgagtcccct gtatctcatt gtcggcatga tgggtcagaa
    13201 ggatgcctcg ggttttcttt cgccgtttcg agggcttgtg aaggaaatcg ttactgtgcc
    13261 gatccctggc gcacacgagg cgccgcacca acccgaggcg ttggccgaga tcgcgcgcaa
    13321 cgtgggattc cgggcagcag cggcgagtga cgtcccatcg gcaatcaagc gaatcgaagc
    13381 aaatgacaga atgccgaaac gcattctgat ctgcggctcg ctttatctcg ctggtcacgt
    13441 gctggctatg cagcaaggcg ttactcagca gtcgaattga tcggcgctgg tcgaagttat
    13501 ccccgggttc gaaaattgtg ctataatatg cgcggggagc cgttaagtcg acctaagtca
    13561 ttgaaagttt gattgttttt tcgcatgtcc ctattgacag cgtgacgctg ttctggcata
    13621 cataagctat aacgcgacag ttgcgagcgg ggcggttgcc catcggaact ggcgcgtttt
    13681 tcatttgtct ccttcaattc cctaatcgga aaacctgatg agtgcacgga aggcctcgcg
    13741 gcagcgggcg cccgactggg tggctatacg ccggctgtac gaggagagca ccattcccgt
    13801 tgccgaaatc gccgaactga acggagtaac ctattcggca atccgaaacc gtcgtctggc
    13861 cgaaaactgg aaaccgcgct cgaaaaaatc gcgcacgaag gtcgcgcccg caaggcgggg
    13921 ccgcacgacg ggtcgtagcg gcctcgtttc gcgactcctc aaggtcgtcg atcagaactt
    13981 gaaactcttg gagatgcgta tggaaacgga cgagccgggg acggccgccg accgtgagcg
    14041 cgagacccgc gcgatcggtg cgctgacccg caccatcgag aagctgacgg agctgcaatc
    14101 ggaagcagtc gctgctcccg ccaaacccgc gaaaacgagc tccggaaccg aaatcgatga
    14161 gaccgacgcg gaccgcctcc gccttgaaat tgcggaacgc attcttcgca ttggcgagcg
    14221 gtacgcatcg cgatgagctg gccgcggcgc tcggcgaact ttcgtccttt gagctgagct
    14281 tcctcgcctg ggactggcag acctgggcgc gcgacgacca gctgccgccg gaggcggatg
    14341 cgcaaggccg accctggcgg acgtggttga ttctcggtgg tcgcggatcg ggcaagacgc
    14401 gtgccggggc cgagtgggtg agagccgagg ttggcctgct ggcccgcggc agcggctctg
    14461 gcagcggacg gatcgcgctc gtcggcgaaa cgctggatca ggtgcgcagc gtcatggtgg
    14521 agggcgtatc aggcctgctg gcggtccatc cgccagatgc aaggccacgg ttcgagcaat
    14581 cgaaacggca gatcgtctgg cccaacggcg tcgttgcgca gctcttttcg gcggaagatc
    14641 cggaaagtct gcgcggcccg caattcgccg cggcctggct cgatgaaatc gcgaagtggc
    14701 gctacgcgga acaggcctgg gacatgcttc agttcgggct tcgcctcgga gccaacccac
    14761 ggcaggtggt gaccacgacg ccgcggccgg tgccgatctt gaaacgcatc atgggcgagg
    14821 acagcaccgt gatcacgcgg gtgcgcaccc gcgacaacgc tgcgaacctg gcgccggcct
    14881 ttgtcgcgga aatggagcgg cgctatgcgg gaacattgct ggggcggcag gagctcgagg
    14941 gtgagttgat cgaggatatg tcgggtgcgc tgtggcggcg ggaatggatc gagcagcacc
    15001 gcctcgccgt ggccccgcag ctgcagcgcg tcgtcgtggc cgtcgatccg ccggtcaccg
    15061 caaccagctc atccgacgcc tgcggcatct gcgttgccgg actcggcccg gatgggcgcg
    15121 cctatgttct ggccgaccgc acgctgcaag gccgtgagcc gcaggtctgg gcgcgcgctg
    15181 ccatcgcggc atatcgcgat ttcgcagcgg accgggtcgt cgcggaggtg aaccagggtg
    15241 gtgatctcgt gcgggatatt ctgcgccaga tcgatccggc cgtgccgatc cgcatggtac
    15301 gggcaacgcg cggcaaatgg gtgagggcgg agcccgttgc ggccctttat gccgagggcc
    15361 gcgtcgccca tgtcgggcat cactcagccc tcgaggcgca gatgctggct ttcgggatcg
    15421 acggacttgc gggtggccgc agccctgacc gtctcgacgc actggtctgg gccctgaccg
    15481 agctgatcct cgactcaccg cctcgtcccg cgatgcggat gctctgaccg gcacgcattc
    15541 tcttagccaa ccgggtgaaa tgatgacgac agtggaacga cagccctcgc ggctggcgcg
    15601 gatgatgcgc gccttgagcc tgcggtcgcc gcgcggccgg gagcggaaag cagccgcgcc
    15661 agggccgctg gtcgccatgg aaggtctgcg gacaccggcc tggatgccgc gcgattatgg
    15721 ggccttcgcg cgcgaaggca tgatgcagaa ccccatcgtg taccgcgcgg tgcgcatgat
    15781 ctcagaagcg gctgcctccg ttccgctcct gctttacgag ggcgagcacg agctcgagga
    15841 gcaccccttc ctcgaattgt tgcggcgccc cggttcaggc cgcacgacga cggacctcat
    15901 ggaggcctgg tacggctacc tgctcgtggc cggcaacgtg tatatccagg cgatcgccct
    15961 ttggggccgg atacaggagc tgcatgttct gcggcctgat cgcgtgcggg tcattccggg
    16021 ttccgacggc tggcccgaag cgttcgaata tcatgtcagc gaccggaggg tgctgatcgg
    16081 cggcgaggtg cttcccggca tcgaccggat tcttcacctc aagctgtttc accctgccaa
    16141 cgaccattac gggctgtcgc cggtcgagcc ggcagcaacc gccatcgatc tccacaacac
    16201 ggcaagccgc tggaacaagg cgctgctcga caattcggcg cggccttcgg gtgcactggt
    16261 ctattccagc ggcggccatc tgacggagga gcagttcaag cgcctcaagg ccgagctcga
    16321 ggcgaatttc cagggagcgc gcaatgcagg ccgtccattg ctgctcgaag gcgggctcga
    16381 ttggaaggcc atgagcctca cgccgcgcga catggatttc atcgaggcgc gccacgcggc
    16441 agcccgcgag attgctctgg cgctcggcgt tccgcccatg ttgcttggca tccccggcga
    16501 caatacgtac gcgaactatc aagaggcgaa ccgcacgttc tggcgccaga cggtgctgcc
    16561 gctggtgaac cgaaccgcaa aatcgctctc ggcctggctc gggccggcgt ggagcgaccg
    16621 cctggagctg cggccggacc tcgatgccat cgaagccctt tcttccgagc gcgaagcgct
    16681 gtgggcccgg gtcgaacgcg ccagcttttt gacccgcaac gagaaacggg cggcggtcgg
    16741 atatggaccg ctgcccggcg gggacgagct cgtatccccg tgaagtctgc gcgaacgcac
    16801 caaattcaac ccgaaactcg gatggatcgg atggagacac tggaaactcg gccgttaatg
    16861 gcggcccggg aggtgaagct gacgtcgctg ggtctcaagg atgtgacgct cgacggtacg
    16921 ttcgaaggct acgcgagcct gttccaccgc gaggacctgg gccgcgacgt cgtgatgcca
    16981 ggcgcattcc gggaaagcat tgccaggcgc ggcgccgcgg gcatccgcat gctgttccag
    17041 catgacgcca accagcccat cggcacgtgg aaaaaaatct acgaggatgc gcgcgggttg
    17101 tttgtccgtg gccagctcgc gacagaggtc ggcaaggcgc gcgaggtcct ctcgctgatg
    17161 cgcgccggcg ccatcgacgg actctccatc ggcttccgga ctgtcaaggg cacgcgggat
    17221 tctcgcacag gcgtgcgccg gctggaaaag atcgatctct gggaaatttc gatcgtcacg
    17281 ttcccgatgc tcccggaagc tcgggtttcg acggtgaaat cgaatccgtt cggcggtcgc
    17341 gttccgaccg aacgggagtt cgagcgctgg ctcacgcagg acgctgggtt cacgcgctcg
    17401 gaggcacgcg cggtgctgcg cgatggcctc aaaggcctac ggctcctgcg ggatgcggag
    17461 caggcctcca gttgggagca gcggctgctg gcgcagatgg cggaagcgac gcggctcctc
    17521 agtcaaaaca tgctcatgag aggatttaca ctatcatgct gaacgaaacg tcgctggagg
    17581 tgaagtccgc aaccaacgcg gatctcagca tcgccttcga agacttcatg cgcgccttcg
    17641 aggccttcaa ggagacgaac gaccggcgcc tcaccgagat cgagcgccac atgagcgccg
    17701 acgtcgtcac tgtcgacaag ttggcgcgca tcgacagagc gctcgatgag tacaaggggg
    17761 ccatcgatga gctgtctcgc aaggcaggac gaccgatgct cggctcggcg gtccgcctgt
    17821 ccggaggtct cgatcataag tccgccttcg agggctacat gcgccgcggc gaaagcaacc
    17881 tgctgcgcag cttcgaaagc aagggactgt cggtcggctc ggattcggac ggcggctacg
    17941 tcgtgccgga ggagacggag cgctcgatca atcaggcagt ccgggatatc tcgccgatcc
    18001 gcgccatcgc cggcgtgcgg caggtgtcgg ggtcggtcta caagaagcct tacgccatct
    18061 caggcgcgga ggcgggctgg gtcggcgaga ctgatgagcg tcccgagacg agcagtccga
    18121 agctcgccga actcgccttc ccgaccatgg agctctatgc catgccggcg gcgacgcaga
    18181 cgttgctcga cgacgctgcg gtcgacatcg accagtggat cgccgaggag gttcgggcga
    18241 ccttcgcggc acaggagggg accgcctttg tcactggcga cggtgtcaac aagccaaagg
    18301 gcttcctcag ctatccgacg gtggacaacg catcctggac gtggggcaac atcggcacga
    18361 tcgcaaccgg ggtgtctgga gcattccccg atacggatcc ggccgacaag ctcatcgacc
    18421 tgatctactc ggtgaagggc gaatatcgcg ccaacgccca tttcgtgatg aatcgttcga
    18481 cccagtcggc cgtgcgcaaa ttgaaggacg ccgacggcag ctatctttgg caaccttcgg
    18541 cccgacctgg tgaggcagca agcctgatgg gcgtcccggt ggccgagtcg gaggacatgc
    18601 cggacatcgc ggccgacagc cacgcgatcg ctttcggtga cttccgccgc ggttacctga
    18661 tcgtcgatcg cgtcggcatc cgcgtgctgc gcgatcccta cagcgccaag ccttacgtgc
    18721 tgttctacac gaccaagcgt gtcggcggcg gcgtgcagga ttttgccgcg atcaagctgc
    18781 tgaaattcgg cgcttagccc gcaaacgaga aggccatccc tcgcgagggt cgccgccggg
    18841 ccctcgcgag atctgttcgc gcgtccatca caccagcgcg agccgagcgg gcggggtttc
    18901 ccccgctgct tcctcccatg gggccccgcc cgcaatctcc tgcaattcga aacggagcca
    18961 tcatgggtct tgttctgacc gctgcgcccg ccgtggaacc gattacggtg gaagacgcga
    19021 agatgcactt gcgcatcgat catggcgacg aggacatgct gctcgccagt ctcatcgccg
    19081 catcacgtct gcaagtcgaa gcggcgctcg atgcggcgct gatcacccaa agctggtcgt
    19141 tggagctgga tgactggccg gacgggcgtg aactgcatct gccgatatgg cctgtgcagt
    19201 ccgtcgaggc cgtacgcatt tcggatcggg atggagaaat ggtcgagctt ggtgcagagg
    19261 cattttcgct cgacggagca agcaagccgc cgcggctcgt ttccatttcc ggtgactggc
    19321 ccggccccgg aactcgcgcg ctcggaattg aaatcctgtt caccgcggga tttggaaata
    19381 gcgcgaatga tgtgccgccg ccgatcaggc aggcgctgtt gatgctcgtc gcccattggt
    19441 acgagcaccg ggagcccgtc gaaacgggca agcccacggc ctcaattccc gaagcgatat
    19501 cggcgctcct catgccgtat cgaacggtgc ggatatgaag acttggccta taggcgccct
    19561 gcgccaccga gtgcagctgg aagctccctc gcgcagcccc gaagaggcag gaggcgctgt
    19621 cgttctctgg gagcccgtcg caacactctg ggccgaaatc attcctttgt ccggaactga
    19681 agtgttccag gcagatggca tttcagcaac ggcggctttt gaagtgcgca tccgttttcg
    19741 tccggatata aatgcagaaa tgcgcttcgt attcggggag cgcgtcctcg acatcaaatc
    19801 ggttcgcgac atcgagggtc gccggcgctg gctaagctgt ctatgcgagg agcgcagctc
    19861 atgaaaatcg ctgtgcggat tgaaaagtcc agaggtcggc gcgagcgcgc attgagtgag
    19921 ctgcttgcgg cggccagatc cggggctcag cgccgtggcg gtggtgccgg gcaagtcgtt
    19981 tcgcttcaaa atgacgaggt gagttatgac gtcatccagc ctggcgctgc agatcgcgat
    20041 tttcgaggcg ctcaggactg acgcgggcgt gctcgctgcg ctcggcggcc cgcgggttta
    20101 cgatcacgtc ccccgcaaga cggactatcc ttacctcaca ttcgcgcaga ccacggtgca
    20161 ggactggagc acaggcgctg acgagggcga tgagcacatc atcacgctgc atgtgtggtc
    20221 gcgcgtggaa agccgcaccc agctgcaaga gatcatcgca gccgtgcgcg ccgccctgca
    20281 cgaccgggac ctgccgctca tcggacaccg gctgatcaac ctcaggtacc agagcgcgga
    20341 aacgcgtcgc gagccggatg gcgaacgctt tcgcggcatc atccgcctgc gggccgtgac
    20401 cgagccgctc gcttgataac ccgaaactct gcactggaaa gagctcagga aatggcagca
    20461 caaaaaggca aggatctcct gttgaaaatc gactcggacg gccttggcgc attcgagacg
    20521 gtagcaggtc tgaggtcacg ctcactttcg ttcaacacgg aggtggtgga cataacccac
    20581 caggaatcca ccggacaatg gcgcgagctg ctggccggcg caggcgccaa aagcgcccgg
    20641 attgccgggt cgggcatctt caaggatgcg gcctcggatt cggcgatccg acaagccttc
    20701 ttcgacggcc tgatccgaac ctggcgggtc gtcattccgg acttcgggac ggtcgagggg
    20761 ccgtttcaga tcacgtcatt tgaattgacc ggccggcacg acggggaagt cgcattcgat
    20821 atcgccctgg agtctgccgg cgagttgtcg ttttcggctc tttgacggag tgagcaatgg
    20881 caaacgcgcg ccgaggggaa atcgaggcct gcctcaacgg gaagacatgg aagctttgtt
    20941 tgacgcttgg cgcacttgcc gagctcgaac aggcgttcgg cgacgacgat atgctcgcgc
    21001 tggcacagcg gttcgaaaaa ggccggctca gcgcaacgga ggcggtgcgc atccttggcg
    21061 ccggattgcg aggcgcaggg cacgacgttt ccgatgaaga ggtgaaggcg atgcgaaccg
    21121 aaaacgggat tgtcgggatc gtcgacatcg ttgcgcggct gcttgcggca acattcggca
    21181 tgcctggtgg ggcagctgag gaaagcatgc cgcgctcgga ggtgcgctcg ctcgaccctt
    21241 tccgtgggac gacgtgatgg ccgctgggct cggtctgttg cggcttccac cggccgcatt
    21301 ctgggccatg acgccaaagg agctggcagc cgcgcttggt gcgctccacg gtccggcacg
    21361 gattgatcca ccatcccgta cggaactccg ccggctgatg caggcctatc cggacgagca
    21421 tcagccaggg cgaatcgaaa gtgaaattca caatggctga acccgtcgaa acttggacgg
    21481 tcgcaatcga tgccgatacg tccaggctgc agcaagagtt ggcgaatgcg acccgcatcg
    21541 gccggcagtt cggaaatgcg ctgacgaatg cctttcaggg tgtggcgctg cgcgggcggg
    21601 atctcggcga agtcctgaaa tcgctgacgc tgagcctatc gcgcatggcc ctgcaggctg
    21661 cattccgtcc gctggagcag ggcctttcat cgctcatcgg cggcgccttc tccggtgtcg
    21721 cattcgcgaa aggaggggtg gtgcaaaatg cgctgcctgt tcctttcgca tccggaggtg
    21781 tgatcgcgag tcctgtaacc tttccgcttg ccggcggccg catcgggata gccggggagc
    21841 gtgggcccga ggcgatcatg cctctgacac gtggtcccga tggcaagctc ggcgtgcgcg
    21901 cccagggtag cgccggtgta tcggtgacga tcaacgtatc gacccccgat gtagaaggct
    21961 tccgtcggtc ggagtcgcag attgcggcca tgctcgcgcg tgccgtcgcc atgggccagc
    22021 ggaacttata gcgctgccgt tcagtgcgtc ttttcagcaa ggaatctcga tggcatttca
    22081 tgagatccgg tttccgaccg acatttctcg cggcgcggtc ggcggtccgg aacggcgcac
    22141 cgaagtcgtc gtgctgggtt cgggccatga ggagcggaac agccgctggg ctgattccag
    22201 acgaagctat aatgcgggtt acggcatcaa ggcactggac gacctgtacg ccgtcatcag
    22261 ctttttcgag gaacggcgcg gtcggctcta tggatttcgc tggcgcgatc attccgattg
    22321 gaaatcctgc ccgccgtcgc aagtgcccgc cgccacagac caactgatcg gcacgggaga
    22381 cggaacgacg gccatcttcc agctcacgaa gacctacggc agcgcgcatg caccctggag
    22441 acgtgaggtg aagaagccgg tcgcgggcag tgtcatcgtc gcagttgatg gcgtcatcca
    22501 gaccgaagat gtggcgttta ccgtcgacag cacgagcggg cttgtcacgt tccagccagg
    22561 gcatatcccg cagcccggct ccaaggtcac cgccggtttc ctattcgacg tgccggtccg
    22621 cttcgacacc gacaagctcg agatcaatct gcagggcttc cggcacggag ctattcccaa
    22681 cattccgatt gttgaaattc ggctatgaaa agtttgcctc cgggcctgca ggcccatctc
    22741 gacagcggcg ccaccacgct ctgctggtgc tggcggctga cgcgccgcga cggagtgcag
    22801 atcggcttca ccgatcacga ccgcgacctg actttcgatg gcacgacgtt cgaggctgcg
    22861 gccggcttca ccgcgacgga gatgaagcac agcgtcggcc ttggcgtgga cgatttggaa
    22921 gtcgaagccg cgcttacctc ggagcgcctg agccaggatg atctcgctgc gggcctctac
    22981 gatgatgcgc gcgtggaaat cttccgcacg aattggagcg atcccgagca gcgcgtgctc
    23041 atgcgcgtcg gcagcctggg ggaggtcagc cgttccggag catcttttcg ggccgaggta
    23101 agggggcttt cgcattacct ccaacagccg aatggccgat tgtttcaatt cacctgcgat
    23161 gctgatctcg gagacagacg atgtggcgtc gatctcacct ctgacagtta tagggcgaca
    23221 gggattgtga cggctgtctc ttccccgcgc gcattcgagg tcagcggcct tgatgagttc
    23281 accgaagggt ggttcgcgcg cggtctgctt acgttcagat caggtgcaaa tcagggccgc
    23341 gcgatcgaaa ttcgccacca ccgccggcgc gatggtgctg cgctcatcga gctctggcag
    23401 gagccggcga aggccgtcgc gattggcgac gaggtcgatg tcaccgccgg ttgcgacaag
    23461 cagctttcga cctgccgtga aaagtttgcc aatgtcgtaa acttccgggg ctttccgcat
    23521 atgcccggca atgattttct cacctcttac gcgcgcaggg gtggcggccg atgaattgcg
    23581 ctcagcgttc tccgacacgc atggcagtcg tgagaatagc gcgctcctgg attggaacgc
    23641 cctatcacca tcaagcaagt ctcattggct gcgggacgga ttgcctgggc ctcgttcgcg
    23701 gcatctggcg tgaactgtac ggcacggagc ctgccacgct acccccgtac acgcgagact
    23761 gggccgaggc gactggtcgc gaaacactgc tcgatgctgc acggcggcat ctcgtcgaag
    23821 tggagaacga aaaagcccag ccgggcgacg ttcttgtttt tcggctgagg gaatgcctcc
    23881 cagccaagca tgtcgcgata ctagcgaccg gcacaacgat gattcacgcc atggagggcg
    23941 tatccgtggc cgaggtcgcc tttgcaccct ggtggagaag gcacctcgcc ggagtctttt
    24001 cgtttccggg gatcgaagac tgacatggca acacttgctc ttgcagctgc gggcgccgcg
    24061 gcgggcagcg cgctgctgcc gtccgggctg accgtccttg gcgccacgat cggcggggca
    24121 accataggtg ctcaggtcgg cgcacttgcc ggctcgttcg tggatcaggc gctgttcgga
    24181 gcatccgggc agagccgcac ttttacgggt ccgcgaattt cggatctgcg cgtaaccgcc
    24241 tcgaccgagg gtgcgcccat tccacgagtc tatggccggg cgagggtcgg tggccaagtg
    24301 atctgggcaa ctgacttcga ggaggaggtc gttacctcac agaccggtgg cggtggcaag
    24361 ggcggaggca gtagcggcgc gtcgaaacag gtcgaatatc gatattacgg caatttcgcg
    24421 gtcgggcttg ccgaaggtga gatttcagga attggccgtg tttgggccga cggccaagag
    24481 ctcgatctca gcagcgtcac ctggcgtctc tataccggca gcgaggatca ggagcctgat
    24541 agcctcatca gtgcacacga gggtgcggaa agcacaccgg cctaccgcgg gctcgcctac
    24601 gtcgttttcg agcggatgcc actcgcctca tacggcaatc gactgccaca gctctcgttc
    24661 gaggtgttcc gcgccgtcga tgattttcac cggaatgtgc gaggcgttgt attgatcccg
    24721 ggctcgggcg agttcgtcta tgccaccgag gaggttacgc ggctcgaagc aggtggcgtg
    24781 caactggcgg agaacgtcca cacgcgccag ggtgggacag attggacggt ttccattgat
    24841 cagcttcaat catcccttcc caatgcaagg tccgtttcgc tgatctcgag ctggttcggc
    24901 actgatctgc gtgcctccca ttgtgagatt cggccaggtg tcgaaatcgc aaagaaatcg
    24961 actaagccac tgacgtggag cgttgccggc ttgtcccgct ctgccgctca cgtcgtaagc
    25021 ctgcacgagg gtaggccggc ctacggtggc acgccttcgg atcagaccgt gatttccgcg
    25081 atacgcgacc tgaagaagcg cggcttttcc gtcgtactca cacccttcat tctgatggat
    25141 gtgcctgcgg ggaactccaa gcccgacccg tacaagggcg gtgcgccggg gcagccagcc
    25201 tatccttggc gtgggcggat cacggtggag ctggcgcccg gacaaccagg aactacggac
    25261 aagactgtag ccgctgctgc tgaagtggcc gctttcgtcg gcacggcgtc cgtctctgat
    25321 ttcgcagttt ctggcgatag ggttgcctac ggcggacagc cggaatggtc ctatcggcgg
    25381 ttcatccttc accacgcttt cctggcaaag gccgctggtg gcgtcgacgc gtttgtcatc
    25441 ggaacggaga tgcgcggcct tacgcaaatt cgcagcgggc ctcaggacta tccgttcgtg
    25501 gccgccctgg tccagcttgc tgccgatgta aagcaagtgc tggggcccga aacgaaggtc
    25561 acctatgcgg cagactggtc tgaatatttc ggccaccagc cgcaggatgg atcgaacgac
    25621 gtttatttca accttgatcc gctgtgggca tcgccagaca tcgacgccat cggaattgac
    25681 ctttactggc ccctgagcga ttggcgcgac ggggcagacc atctcgacgt cttggccggt
    25741 gcccgctccg catatgatct tgattacctg aaatccaata ttgccggtgg ggagggatac
    25801 gaatggtatt atgccagtgc ggccgatcgg aatgagcaga ggcgcactcc gataacggac
    25861 ggccaaggga aaccctgggt cttccgctac aaggatctca aatcctggtg gctcaaccag
    25921 catttcaatc gacctggtgg cgccgagcag gcaacaccca cggaatgggt gccccaatcg
    25981 aaaccgatct ggttcatgga aatcggctgc ccggcgatcg acaagggatc caaccagccc
    26041 aatgtcttcg ttgatccgaa gagtgccgag acggcgctcc cgtacttctc gaacgggaaa
    26101 cgggatgatt tcattcagcg ccgctatctg caggcgctga tcgaagcctt cgatcctgag
    26161 catcccggag cgctgccggc gctcaatccc atttcagaca tctatggtgg gccaatggtc
    26221 gacccatcgc gaatgcatgt ctacgcctgg gatgcgcgcc cattcccggc ttttccggct
    26281 gataccgaga cctggggcga cggcgccaac tggcagcgag ggcactggat caccggccgg
    26341 atcgcgagcg caccggttgc cgacctcgtc agacgaatac tcgatgattc gggctttcat
    26401 gcgcatgacg cgagcgcact cacgggcatt ctgcccgggt acacgatcga ccgccttatg
    26461 gcgccacgcg aggcgatcca gcctctggag ctcgcttact tcttcgacgc tgtcgagacg
    26521 ggtgagcgaa tcgtattccg cccaaggggc gccgggcgcc aggtcatcga ggtttccacg
    26581 gatgacttgg tggaagagcg gacgggggcc aatctcgtca ccattacccg cgggcaggaa
    26641 accgagcttc ctggttcggc aaaaattact tatgtttcgg ccgcgaatga ctaccgccag
    26701 gcggtcgtcg aggcgcgccg gttggcggga gcgagcgccc gcgtttcaca ggccgatctg
    26761 gcgctcgttc tggaagatga acaggcggcg gctatcgccg atgcgtggct tttcgaggcg
    26821 tgggccgcgc gggaacgggc attattcgtt ctgccgccga gcaagatggc catagagcca
    26881 tcagacatca tcgcgatctc cgaaggagga cgctcgcggc ttttccgggt gaaagaaatt
    26941 ggcgaccacg gcgcacggca ggccgaggcg ctgagcatag acccggcgct ctattttctt
    27001 ggggaaggcc ccgcgcgcag gcatcggata tccccgccca cggcatcagg gcagccgctt
    27061 gggctgcttc tcgatctgcc gctcctcaga ggagatgagc ctgagcacgc cgcgtatttc
    27121 gccgcatcac agtcgccctg gccggggggc gttgcgcttt tccgctcgcc gactgaaaca
    27181 ggctatacgc tccagggtgt tgcggaggtg ccggcaatca cgggcgagct cctcgatgcc
    27241 ctgccgccag ggccggtcgg gcgattcgac aaggcggccc gcttgcgggt gcagctcgcc
    27301 gtcggcgagc tcgcgtcggc tgaggaactg cagctgctgg ccggggccaa tgcggcggcc
    27361 gtgcgcaacg ctgccggcga ttgggaagta ctgcagttcc agtctgctgt gctcgtcgga
    27421 ccgaaaactt acgagctgtc cggctttctg cgcggccaag ccggtactga aggtgcaatg
    27481 gaaagcgaga tcccgcccgg cgctccgttc gtgctcatca ctcaggcttt ggtgcgggtt
    27541 gacctggcgc ccgcggatgt cggcctcgag tacaattggc gcttcggccc cgccaatcgg
    27601 gatctaggtc acccgagcta cgtgcaaacg cggcatgcct tccgcggggt tgggctgcgt
    27661 ccgttaagtc cggtgcatct gcgcgggaga agatccctga acggcgatct ggctctggct
    27721 tggataaggc gaacgcgtgt cggcggggat agctgggaga cggccgaggt gcctcttgca
    27781 gaaaccgtcg aacgctacga ggtcgacatc ctcgacggcg catccgtgaa gcggaccata
    27841 tccacgaccg agcccgccac catctatacc gcggatcagc agatggccga tttcggcgtc
    27901 ctgccgtcgg cgctcaatgt ccgcgtctac cagatgagca gcgtctgggg gcgcggatcc
    27961 ccctgcagcg cggtcgtctg acgaccgctg tcagtttcca gaaccctgta gcccaaggag
    28021 atttgttcca tggagcagcc ggcatggctc gagcacgcct ggcgggaagc tggcgtacgc
    28081 gaggagccag gatctgcgag caacgatcgc atactgcagt tttttcggga tgtcgggcat
    28141 cagacagtca caagcgacga ggtcgcctgg tgcgcggcat tcgtcggcgc ctgcttggag
    28201 cggtccggat ttcggagcac acgctcgttg ctcgcgcgct cctacctgga gtggggtgag
    28261 cctctggcga aggcgcggat tggtgcgatc gcggtcctca gccgaggcag tgatccgggt
    28321 cagggccacg tcggtttcgt tatcggtgcg acggcagagg agctcttcct tctgggcggc
    28381 aatcagcaga atgcggtctc ggttcagtct ttcgaaatcg acaggctgct tggattccgc
    28441 tggccagctg gagctgttga aaagagctca gcaacggatg agctgttcga cctggcactc
    28501 gcccacgtcc tggaaatgga gggcggctgg acgaatgatc cccacgaccc tggcgggccg
    28561 actaatctcg gcatcacact cggggtctac gcggcgtcga agggcacgac gatcacggat
    28621 gccaatcgtg aggatctcat tggcgagctg aagcggctcg atgccgcttc cgcgcggccg
    28681 atctactacg agcgctattg ggttccgtcg cgggccgcag agctgccccc gccgatcgcg
    28741 ctgatgcact ttgatgccgc cgtgaatcat ggggtcggaa atgccgcccg gatgctgcag
    28801 caggcattgg gcgttgccgt tgatggtgag atcgggccgg aaaccctcgg agcagcccgc
    28861 acccaccccg tcatggaaac tgtccatcgc tacgccgaca tcaggcgggc gcgctaccgc
    28921 agcctcagcc atttctggcg gttcggacgc ggctggttga gacgagtcga tcgaacactc
    28981 gctgccgccg agcggctcgg tgatgccgca accaaagctt cccggcaact tcagaaagga
    29041 gcaggacaga tgtcagcaac tgaaggcgcg gccgccgaca gcaagtggtg ggggcactcc
    29101 atgacgatct ggggtgcaat catcaccgca gcatcaacgg tacttccggc catcggtccg
    29161 ctcttgggga tcgatatcac cccggatctc gtcaacgagt tcggcgacca agtgacgaac
    29221 gtgctgcagg ccataggcgg gctgattggc atcatcatga ccatctacgg gaggacgcgg
    29281 gcaagcacgg gactcgggcg taaacagatc accctccaac tctgaatcgt tcaggcggcg
    29341 ttcaggtgcg cagctcaacg atcaatacag ggctgaaggc cgatgcgcat gccggtcgtg
    29401 tggggcatca gcgcgccttg tgatcgtggc ggttggcact caccatgggt gccagccgcc
    29461 tcgatgctcg caagggcttg cgagcaacag gctaggagga tcagccattt tggggtatat
    29521 accaaggtcc gaacagttcc agtctttagg tgggggaaac ggcaggttgc gcgcccttgt
    29581 cgttgaggac gatcccgatc tcaatcgcca gctcaagcag gcgttggcgg atgccggctt
    29641 tgccgtcgac tcggcgagcg acggcgagga gggatatttt ttgggcgaga cggagcccta
    29701 cgacgtcatc atcctcgaca tcggcctgcc gaagatggac ggcatcagca tcctcgagca
    29761 gtggcgccgt gcgggccgca acatgccggt catcatcctt acggcgcgcg accgttggag
    29821 cgacaaggtg gcgggcatcg atgccggtgc ggacgattac ctcgccaagc ccttccatat
    29881 ggaagaactc ctggcgcgcg tgcgtgctca ggttcgccgg tccgcaggtc acgccaagag
    29941 tgagctcgag tgcggtccgc tgcgcctcga tacgcgaacg gcccgcgtca cgtgcgatgg
    30001 tcagccgatc aagctgacgt cgcacgagta ccggctgctc gcgtacctca tgcaccacaa
    30061 cgagcgggtg gtgtcgcgca ccgagcttgt cgagcatctc tatgaccagg acttcgatcg
    30121 cgacagcaac acgatcgagg tcttcatcgg ccggctgcgt aaaaaaatcc cggctgattt
    30181 gatcaagacc gtccgcggtc tcggttaccg gctcacccag gagcccgatg aggcttaatt
    30241 cgctcgcctt ccgactgttt gcgacggcga tcgtctcgac gatgctgatc ctgccggtgg
    30301 cgggactgct gatctattcg gtgtaccggc aggaagccga ggaaggcttc gacgagcgcc
    30361 tgcgccagct cctgacaatc gtccacgccg acagcattga cgagtcttcc tcggagccgg
    30421 gcacgcccgg gcaggtcggt gagcccctct ttttcatcac gcattcgggc tggtactggc
    30481 agatcaagcc gctgaatgga gcgccgggcc agacaaaggc ttccccttcg cttgcaaact
    30541 tcgtgctgcc gtcgccgttc gaggagggca tcgagcccga tgtgcaaggc acgcgatggc
    30601 tcgacgtcct gggaccgaac aatcagcctg tacgcattgc cgagacgatc tatgctttcg
    30661 gcgaggattc tagcggacct cagtattcga tcgcggttgc cggcccgctc gattggctgg
    30721 aggcacgcgt tgcgagtttt gggacccagc tcgcgttcgc tttgacgctg gccggtatcg
    30781 ctctagtggc cgtgacgctt ctccaggtcc gcttcggcct ccttcccttg cgcgccatag
    30841 agaacgggct tgcggccgtc cgctccggaa aatcgacgcg cctcgaaggt gagctgccag
    30901 ccgaaatcga gccgctccag catgagttga atgcgctcat ccagtccaac gaggaggtca
    30961 tcgagcgtgc ccgcacgcag gtcggcaatc ttgctcatgc gctcaagaca ccgctagccg
    31021 tcatcaccaa cgaggcgcgc gaggataaaa gcccgctcgc gcagaaagtt gcggagcaag
    31081 cccaggtgat gcgcgaccag gtgaaccact acctcgaccg cgcccgcatg gcagccagtg
    31141 ccggttcctt ggtgcgtgtg actgacgtgc tgcctgtcgc ggaagctttg cagcgcgctc
    31201 tcgagcggat ctaccacgaa aaggggatcg caatccgact tgattgtccc gaggacgccc
    31261 gcttccaggg ggagcggcac gatctcgagg agatgctggg caatctgctc gacaatgcct
    31321 gcaagtgggg tcgaaggagc atccgtgttt cgatcagcgt gcggcgcccg gagcttcggg
    31381 gcgcgtcgcc acgcctcatc gtgacgatcg aggacgacgg ccccggcctc acggaggatc
    31441 agcgccgccg gatcggtaag cgcgggctgc gtctcgatga aaccaagccg ggctccggtc
    31501 tggggctctc gatcgtcagc gacctggcgc tgtcataccg tggccggctc gagcttggcg
    31561 catcgcgcta cggcgggcta ctcgcgaagc tcgatctccc cgctgcctga agacgacagg
    31621 acctcagggc catttcgcta cgggcggcat cgaggacaga atgctgtcca catttccccc
    31681 ggtcttcaag ccgaacacgg ttccgcggtc gtagagcagg ttgaattcca cgtagcgccc
    31741 ccgccggacc agttgctcct cgcgctcggc ttccgtccag ggtttgtgcc agttgcgcct
    31801 gacgagctcc ggatagatgt tccggaacgc ccgcccgacg tcctgcgtga aagcaaatgc
    31861 ggcttcccac ccgccatctg cttctggcgg cgtcaagtaa tcatagaaga tgccgccgac
    31921 gccgcgcatt tctttccggt gcggtaggta gaagtactca tcacaccatt ctttgaagcg
    31981 ctcataatcc gcaaccggat ggggcgtgca ggccattcgc atcgcttcgt ggaacgcgat
    32041 cgtgtcggga tcggactgcg tgcgccgggt ctcgagcagg ggtgtcagat ccgcaccgcc
    32101 gccgaaccac catttcgtcg tcaccaccat gcgggtgttc atgtgcaccg cgggcacgtg
    32161 cgggttctgc atgtgcgcga tcagcgagat gccggaagcc cagaagcgtg gatcctcgct
    32221 ggcaccgggt atctgcgacc ggaactccgg cgcgaactct ccgaacaccg tcgaggtgtg
    32281 aacgcccacc ttctcaaaaa cccgcccttt cataatggac atcagtccgc cgccgccggg
    32341 tgcccccgtg tgatcggtcc ggctccaggc cctttcgatg aaccgtcctg gcggtagctc
    32401 ggaaagtgga gaacccggcg ggagctcgtc ctccagacgc tcgaaggcgc tgcggatctc
    32461 atcccgtagc gccccaaacc acctggaggc gcgatcacgc cattgatcga gaaggtccgg
    32521 ttgaggcagc atgtcctatc ctgaagtcat tcttctgacg tggcttggga ctaacgcgga
    32581 acctgcattc aatcaatcga agccggtccg ccggccgcct tgtgacagcg gccttttgct
    32641 caactcggca gttcttgctg agcgtccgca agcctgcact ggctcaagtc acgaaaggaa
    32701 tcgatgtacc gctggttcga aacgcgtatc gacgccttcc ctcaagaggt cccgcagcag
    32761 ccacccaaca ggctgccggc gttctactgg catttcatcc ggccggtctg gccggttttt
    32821 gtgttgctcc ttgtcgtcgg cctcataggc tcgctcatcg aggtcgcgct ttatgccttc
    32881 atcggcgata tcgtcgacag gatgaaggag gcgccgtccc ctgaaacatt tatcgcggac
    32941 aacggcctgc tgctcctgct gatggccttt gtcgccctca tcctccgccc gctggtgtct
    33001 acggctcacg atctgaccaa gaaccagatg attgcggggc cggtgaatac gcgcgtgcgc
    33061 tggcagtccc atcgctacgt cctgcggcag agcctcggct ttttccagaa tgacttcgca
    33121 ggccgggtcg cagcgaaggt cttgcagacc ggccatgcgc tgcgggaatc cgtggtgcag
    33181 atcatcgata gcctttggtt cgcagtcgtg cactggggcg cggcgatcgt tttgttcgcg
    33241 gatgccgatg ggcggctcgt tatcccgctg gtgatctggc tcgcggctta cggggtcacg
    33301 atctggtact tcgtgccgcg catccagcgg ctgtcgttca atgcatcgga agcgcgctcg
    33361 gtgctgacgg gacgcgtcgt cgacagctac acgaacatcc tgacggtgaa gctcttcgcg
    33421 catgcggaac gggaggatga aagcgcgcgg tgggcgctac agaaccacct cgatcggtat
    33481 ctggaaagcc agcgcaatct cacctgcatg gagttcgtgc tctactcgat caatggcgcc
    33541 ctgatggtct cggtcacagg ccttgcgatc tggctgtgga gcaccggtgt cgtgacgatc
    33601 ggagccgtcg cattggccac ggcactcgtc atcagactcg tcggcatgtc cggctggatc
    33661 ctgaccacgg tggcctcggt cttcgaatcg atcggcaccg tagaggaagg catggagacg
    33721 atcagccgcc ccgtcgacgt cgtcgatcgg cccgacgcga agccgctcgt cgtcaaacgg
    33781 ggcgagatcc gattcgagga cgtcagcttc cactacggca agcatggtgg cgtcatcgac
    33841 cgcatatcgt tgcgcatcgc tccgggcgag aaagtcgggc tcgtggggcg gtccggtgca
    33901 ggcaaatcga cactggtcaa tctcttgctt cgcttctacg acctggaagg cgggcgcatc
    33961 ctgatcgacg gtcaggacat tgcttgcgtc acacaggaca gcctgcgggc gcagatcggc
    34021 atggtcacgc aggacacctc cttgctgcat cgttcagtga tggagaacat tctctatggc
    34081 cgccccgatg ccggcgaggc tgcggccata gctgcggcac gcaaggcgca cgcgcacgac
    34141 ttcatcgtcg gcctgcggga tgcacgtggc cggactgggt acgccgcgca ggtcggcgaa
    34201 cggggtgtga agctgtccgg cggccagcgt cagcgcgtgg cgatcgcccg ggttctgctg
    34261 aaaaatgccc ccatcctcat tctcgacgag gcgaccagcg cgctcgactc ggaggtggaa
    34321 tcggcaattc aggagcagct ctacaacctg atggaaggca agacggtgat tgccattgcg
    34381 caccgcctgt cgacaatcgc cgcgatggac cggctggtga tcatggacga gggccgcatc
    34441 atcgagcagg gcagccatgg cgagctcctc gctcgaggcg gactatacgc cgagctgtgg
    34501 gcgcgccagt cgggtggctt cctcgccaag gaggcagcgg aatgaaggaa aagcctaatt
    34561 cgaggaattt cgtgatctcg cgggcttcgg cgtgatcctc aaggttcgcg ccggaaaaag
    34621 gtgtcgacgc gtagcccggg aatcagcggc ccggcgtcct cgaactcgat cgtaacctcg
    34681 cgcacctcgg cgtcatcggg ccggcggggt ccccgtgatg tcatatgcgg accagccagg
    34741 accggcgcca tcgcggttac cttgccctcg atctcctggc ctggaaaagc
//
LOCUS       DASVDU010000012        10298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_939077, whole genome shotgun sequence.
ACCESSION   DASVDU010000012 DASVDU010000000
VERSION     DASVDU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939077"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF226_01005"
     CDS             complement(<1..792)
                     /locus_tag="VF226_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7072596.1"
                     /translation="MRYSVFNLLRQAATGHQGWPRQWRDPEPKPAYDVVIIGAGGHGL
                     ATAYYLAKEHGIRNVAVLEKGWLGGGNTGRNTTIIRSNYLWEESEAIYEHAMKLWETL
                     SDELNYNVMYSPRGVMMLAHNVHDVQSFKRHVHANRLAGIDNEWLTPEEAKAFCPPLN
                     ISPNLRYPVLGAALQRRGGVARHDAVAWGYARAADKLGVDIIQNCEVTGIRRGADGAV
                     EAVETTRGTIKARKVGIVAAGHTSVIAAMAGFRLPLESFPLQALVS"
     gene            complement(1418..2431)
                     /gene="proX"
                     /locus_tag="VF226_01010"
     CDS             complement(1418..2431)
                     /gene="proX"
                     /locus_tag="VF226_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HEX7072597.1"
                     /translation="MKGLRKGLLTLAAAALVGSMACMPVLAQDAPGAGKTIKMARATW
                     DTGWFHAEIYKQLFEELGYTVEGPTTLDNPPFYQAVGLGDVDLWVNGWFPLHDSYRDA
                     FEENAEIIGTVAASGALQGYLVDKASAEKYNIKSLDDFKRDEVKEAFDRNGDGKADLV
                     ACPPGWGCELAIDKHLKKYGLKDHINEVKASYSASMADAVAAYKNGESILFYTWTPNW
                     TVNELKPGEDVVWIPVPEKPTKENSVSGLESCVADPCVMGFVANDIVPVANSEFLKAN
                     PAIATLLKEVSIPLEDIFEQNAAMFKGADKPEDIEKQASDWIKKNRDMVDGWLEAARK
                     AAG"
     gene            complement(2531..3460)
                     /locus_tag="VF226_01015"
     CDS             complement(2531..3460)
                     /locus_tag="VF226_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HEX7072598.1"
                     /translation="MFSPADLVQIPIDDWVNSLVRDFLVPNFRSFFRALQAPVTSVLN
                     WVDAFLHWLPMLAVTAILTVIADRTAGRGVAIFTLLALSFIDMTGLWPETMTTLSMVI
                     TSVLFCAVMGIPLGIWAARSDRVLFVMRPILDIMQTIPSFVYLVPIVMLFGVGMVPGI
                     IATIIFALPPIVRLTNLGIRNVRADLIEAAEAFGSTGWQMLWEVQIPLALRTIMAGLN
                     QTLMLALSMVVIAALIGAGGLGLTVYTGLGRLNVGAATAGGIGIVLLAIILDRITQAL
                     GEKNSETAPSLWQTLCSLFSWGLEAKQEKDKTA"
     gene            complement(3444..4712)
                     /locus_tag="VF226_01020"
     CDS             complement(3444..4712)
                     /locus_tag="VF226_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7072599.1"
                     /translation="MKPKLRIENVFKIFGERPEEALKALEQGKSKLEILEETGQTVGV
                     KGVSFEVNEGEIFVVMGLSGSGKSTLVRMLNGLIPATSGRILIDNEDVTRCSAERLRN
                     IRRNKIAMVFQHFALFPHKTVLENVAYGLKIKGEVAHRRREKALKALEQVGLAAYADS
                     MPGELSGGMQQRVGLARGLATDPEILLMDEPFSALDPLIRREMQEELLHLQKVLNKTI
                     IFITHDLNEALLLGDKIAIMKDGAFVQVGTAQEIVDRPADEYVAAFVADVDRGRVFQA
                     SHVAGRPVTVEQSEIGAAEKALQLMEKHNRNALYVVDKGKVAGVVAYQELAVAAREEG
                     RTDLKEVMVTDVPVVHPETPLNELYAAASAGYPIAMTDSHGRLVGVIEVEAVFSQLSG
                     CASENGATSGNECASQAKTQGSQDRNVQPR"
     gene            5805..6467
                     /locus_tag="VF226_01025"
     CDS             5805..6467
                     /locus_tag="VF226_01025"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing Zn-dependent protease"
                     /protein_id="HEX7072600.1"
                     /translation="MNRVWLACVLAVVFATATGAIAIRSGGRAAPTTNLAISKAVAKV
                     NYYDVRGTTLEELRQDVFSRGPYDKEANTRFAGWAEWRIQWWFDRREVAGGCAVANAS
                     TETHIEYTLPRWVDEDAAPPELRASWKRFIDALTEHEEGHGRLARELAEQIEFAIRSL
                     PPEPTCEELDRKVNELANRLIREDKTQEAYDRETGHGETQGAAFPSILVHATSKTAAV
                     SE"
     gene            complement(6485..7666)
                     /locus_tag="VF226_01030"
     CDS             complement(6485..7666)
                     /locus_tag="VF226_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX7072601.1"
                     /translation="MATTAEQVRSYRGPAILSFGFRPFFLSAAIWAALVMVLWLPVLA
                     GQLVLPTALGPIEWHAHALLYGFVPAAVAGFLLTAVPNWTGRLPVTGKPLLALFLLWL
                     AGRIAVFTSGWIGAPLAAVIDLSFLATLAAVVAREIIAGKSTRNLKVLIVLGVLFLGN
                     VTFHAEILLGLGAEYGIRAGIAAIILLIMLIGGRIIPSFTRNWLVRRSPGRLPVPFSR
                     FDAAVMAFSGAALALWIVLPEADATAVLAVFAGLLNVVRLGRWAGERTTAEQLVLVLH
                     VAYAFIPLGFLLLATSIWAPEVLLPSGALHAWTAGAMGLMIMAVMTRASLGHTGQPLT
                     ATRGITAIYIATFIAALARIVAGFGVAREPMLHLSATAWVVGFAGFAVIYAPLLTRPR
                     G"
     gene            complement(7781..8083)
                     /locus_tag="VF226_01035"
     CDS             complement(7781..8083)
                     /locus_tag="VF226_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072602.1"
                     /translation="MTLGELLAAWQDDAVILDALIGLDDLALLARLKAAAEEDGSDLA
                     TVVREEVGRFVQLADDETWLMLMGRLAKAEDPAKVGLKLMLEYGLARNSAEQGTAA"
     gene            complement(8080..8928)
                     /locus_tag="VF226_01040"
     CDS             complement(8080..8928)
                     /locus_tag="VF226_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7072603.1"
                     /translation="MGCSARGALANAQRTKIAVNGVTIPHDMISREAQYHPAPTPLAA
                     WTAAARALVVRELLLQEARRIGIEPEPETDGEGRRETDEEALIRGLIDREVTTPEPDE
                     ETCRRYYRQNRGRFRSADLFEASHILLAAHPKDEEGRSAARNKAEQLIADLRESPQLF
                     ADFARIHSACPSAKSGGNLGQIGPGDTTPEFEAALRRLAPGEMTSEPVESRYGFHIIH
                     LTRRIDGRELPFELVRERIAAYLTERSRRTAIAQYVARLAAASEVSGISLPTPADLRV
                     SGGPVQ"
     gene            complement(8933..9664)
                     /gene="narI"
                     /locus_tag="VF226_01045"
     CDS             complement(8933..9664)
                     /gene="narI"
                     /locus_tag="VF226_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX7072604.1"
                     /translation="MRDTLNYIIFGWYPYVCLTVFVVGSILRFDRDQYTWKSGSSQLL
                     RRRQLMLGSNLFHIGILIIFVGHFVGLLTPIWVFETIGISHTAKQIMAIVVGGVAGLA
                     CLVGIVMLAHRRLMDPRIRATSSFADTGILLILLAQLLLGLATIPLSMGHLDGGEMVR
                     FMNWAQGTLLLRADTWMLVADAHPVFKAHLFLGMTIFLLFPFTRLVHVWSAPVWYLGR
                     RGYQVVRTRRPLASEGRAAAIEPAE"
     gene            complement(9705..>10298)
                     /gene="narJ"
                     /locus_tag="VF226_01050"
     CDS             complement(9705..>10298)
                     /gene="narJ"
                     /locus_tag="VF226_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX7072605.1"
                     /translation="WDLYELQERYILLFDRTRSLSLNLFEHVHGESRDRGQAMVDLRQ
                     LYERHGLELATNELPDYLPLFLEFLSVIPEAEAKELLGETAHILEAIRQRLKKRKAPY
                     SSVFSSLQALSSSAPAGELLQALLAAPDDDPNDLSALDAAWVDEEVRFGPGAAADCGA
                     ENLVSKLRAARRPAAGVAPRDGQTNHPTITYTRSARA"
BASE COUNT         2063 a         3284 c         2995 g         1956 t
ORIGIN      
        1 cgacaccagt gcctgcaacg ggaagctttc gagcggcaag cggaacccgg ccatggccgc
       61 gatcacgctc gtgtgccccg ctgccacgat gccgaccttg cgagccttga tcgttccccg
      121 cgtggtctcg accgcctcga cggccccatc ggctccgcgg cgaatgccag tcacttcgca
      181 gttctggatg atgtcgacgc cgagtttgtc ggctgcacgc gcgtagcccc aggccaccgc
      241 atcgtgccgc gctacgccgc cccgacgctg tagggccgca cctagaaccg ggtagcggag
      301 attgggtgag atattgagtg gcggacagaa ggccttcgct tcctccggcg tcagccattc
      361 attgtcgatg cccgcaagcc gattggcatg aacgtggcgc ttgaagcttt gcacgtcatg
      421 cacattgtgc gccagcatca tcacaccgcg cggggagtac atcacgttgt agttcagctc
      481 gtcggagagc gtctcccaga gcttcatggc gtgctcgtag atcgcctcgc tctcttccca
      541 aaggtaattc gagcggataa tcgtcgtgtt gcggccagta ttcccgccac cgagccagcc
      601 cttctccagg acagcgacgt tacgaatgcc gtgttccttg gcgaggtaat aggcggtcgc
      661 gagcccatgc ccaccagcac cgatgatcac gacatcatag gccggtttgg gctcaggatc
      721 gcgccactgc cgcggccagc cttgatggcc ggtcgctgcc tgtctgagca ggttgaacac
      781 cgaatatctc aacggatgct ccccgttaca ccacggccga gatggctcgc cggcttcaag
      841 ccgtcatcgc acctcggcgc gcacgtggcc tggaagagac agacggatgg cgctgaaaag
      901 ctcgtctggc catgcgtcgc cttgtcctct ccagggcgca accctaaacg gatcggacgc
      961 cgtgtccagt gcggcaactg gcgtcttctt tcccgtttat ccgccaatgg ctggtcgaaa
     1021 gacagggctg tccgactcac tttggctggt cactttccgc cacaggagct gccgggtgct
     1081 gaatcgccag atcggttgcg aaaatttggt tgcgcggcgt tttggctctt ctttcagcac
     1141 tggactggcg ctgcaaatca ctttttgccc gccagaacgt tattcttcat agcccgaagc
     1201 gagtctagta gagctgcaat gtccgcgtcg cccgccgatc ggaccgaaac gcacagatcg
     1261 gctttctgct cacgcctgaa gtctcgtttc tgacgccgcg gggcggagct gtgtgcgaca
     1321 gctccgcccc atggtcttaa gccctatggt caagtcacat ttgacctttc attggcttgc
     1381 ttgttcagca gcccctgaaa tggctattca cggcaggtca acccgctgcc ttgcgggccg
     1441 cctcgagcca gccatcaacc atgtcgcggt tcttcttgat ccagtcggac gcctgcttct
     1501 cgatgtcctc gggcttgtcg gcgcctttga acatggctgc attttgctcg aagatgtctt
     1561 cgagcggaat ggatacctcc ttgaggaggg tggcaattgc cggattggcc ttcaaaaatt
     1621 cagagttggc gaccggcact atgtcgttcg cgacgaaccc catcacgcag ggatcggcga
     1681 cacagctttc aagacccgag acggaattct ccttggtcgg cttctcggga accgggatcc
     1741 agacaacgtc ctcgccaggc ttgagctcgt tgaccgtcca attcggggtc cacgtataga
     1801 ataaaatgga ttcaccattt ttatacgcag ccaccgcatc ggccatggac gccgaatagc
     1861 tcgccttcac ctcgttgatg tgatccttca gaccatattt cttgaggtgt ttgtcgatcg
     1921 cgagctcgca cccccagccc ggcgggcagg caaccaaatc ggccttgcca tcaccattgc
     1981 ggtcaaaggc ctccttgacc tcatcgcgct tgaagtcgtc cagcgacttg atgttgtact
     2041 tttcagcaga ggccttgtcg accagatatc cctgcagcgc accggaagcg gcgaccgttc
     2101 cgatgatttc cgcgttttcc tcaaatgcat cgcggtatga atcgtgcaat gggaaccagc
     2161 cattcaccca cagatcaacg tcgcccaatc ccacggcttg atagaacggc gggttatcga
     2221 gcgtcgtcgg accttcaacc gtgtagccga gctcttcaaa caactgcttg tagatctccg
     2281 cgtggaacca gccggtatcc catgtggccc gggccatctt gatcgtcttc cccgcccctg
     2341 gggcatcctg agccaggacc ggcatgcatg ccattgaacc tacaagcgct gccgctgcca
     2401 gcgtgagaag gccctttctc agccctttca tgtgtaattc tccgtattgc tacgtcagag
     2461 gtttgtggcg atcagcaccg ccacggcgaa ggcggaacgc actcgagagc ccattccgcg
     2521 aaaatcgtgc tcatgcagtt ttgtctttct cctgcttggc ctccagaccc caggagaaca
     2581 gcgagcacag cgtctgccaa agcgagggcg ccgtttcgga attcttctca cccagcgcct
     2641 gggtgatgcg atccaggatg attgcgagca ggacgattcc gataccgccc gctgtggccg
     2701 caccgacatt gaggcgcccg agacccgtat agacggtcag accaagaccg ccggcgccga
     2761 tcagagcagc gatgacaacc atggacaatg ccagcatcag cgtctgattg agacctgcca
     2821 tgatggttct gagcgcgagg ggaatttgca cctcccagag catctgccac cccgtcgagc
     2881 cgaatgcctc tgcggcctca atcaaatccg cgcggacatt ccggatgccg agatttgtga
     2941 ggcgtacgat aggtgggagg gcaaaaatga tcgtggcgat gatgcccgga accattccga
     3001 cgccgaagag catcacgatc ggcacaagat agacgaacga cggaatggtc tgcatgatgt
     3061 cgaggatcgg ccgcatcaca aacaggactc gatcgcttcg agcagcccag ataccgagcg
     3121 gaatccccat cacggcgcaa aacagcacag acgtgatgac cattgacagc gttgtcatgg
     3181 tttccggcca cagccccgtc atgtcgatga acgacagcgc cagaagggtg aaaatcgcaa
     3241 cgccgcgccc ggctgttcga tctgcaatga cggtcaagat cgccgttacc gccagcatcg
     3301 gcaaccagtg gaggaaggcg tcaacccaat tcagcaccga tgtcactggt gcctgtagcg
     3361 cccgaaagaa ggatcggaaa ttcggcacca ggaaatcgcg caccagcgag ttcacccaat
     3421 catcgatcgg aatttgaaca agatcagcgg ggctgaacat tgcggtcttg gctcccttgc
     3481 gttttcgctt gcgacgcgca ctcgtttccg gaagtggccc cgttctccga tgcacagccc
     3541 gagagctggg aaaaaacagc ctcaacctcg attacgccaa cgagtctccc gtgactgtcg
     3601 gtcatcgcga tcggatagcc tgcgctggcg gcggcataga gctcattcag cggcgtctcc
     3661 gggtggacca caggcacatc agtcaccatg acctctttca gatccgtccg gccctcctcg
     3721 cgagcagcaa ccgccaattc ctgataagcg acaaccccag cgaccttgcc tttgtcgacg
     3781 acgtaaaggg cattccgatt gtgcttctcc atcagctgga gcgccttttc cgcagctccg
     3841 atttcgctct gctcgactgt cacgggccgt ccggctacgt gcgaggcttg gaaaacccga
     3901 ccgcggtcga cgtcggccac aaaggcggcc acgtactcgt ccgcaggccg atccacgatt
     3961 tcttgcgccg tccccacctg aacgaaagcc ccgtccttca taatggcgat cttgtcgccg
     4021 agaagcagcg cttcgttgag gtcgtgggtg atgaagatga tcgtcttgtt caatactttc
     4081 tgcaggtgca gaagctcctc ctgcatttcg cgacggatca aaggatcgag cgcgctgaac
     4141 ggctcatcca tcaggagaat ctcgggatcc gtcgcgaggc cacgagccaa gccgacccgc
     4201 tgttgcatgc cgccggacaa ttcgccaggc atgctatcgg cgtaagccgc aagccccacc
     4261 tgctccagcg ctttgagtgc cttctcacgc cgcctatgag ccacttcgcc cttgatcttc
     4321 agtccatacg caacgttttc gaggaccgtc ttgtgaggaa acagcgcgaa atgttgaaat
     4381 accatggcga tcttattgcg ccggatattc cgcaaccgct ccgccgaaca ccttgtgacg
     4441 tcctcattat cgatcagaat tcgtcccgaa gtggccggaa tcagcccatt gagcatgcgc
     4501 acgagcgtcg atttaccgga gcctgacagc cccatgacga cgaagatttc cccttcgtta
     4561 acctcgaagc tgacgccctt gacaccaacg gtctggccgg tttcctccaa tatctcgagt
     4621 ttcgacttgc cctgctccag tgccttgagc gcttcttccg gcctttcacc gaaaattttg
     4681 aagacgttct cgatgcgtag cttgggtttc ataagccctc ttattcttta aactgaattt
     4741 cgatcggcct aggcaacaaa gcctaccgat cccagtgctc cgaaagtctt ctctcgcgtc
     4801 ccggtgcggc gggcgccaat tcgcaagcaa gacgcatcag cactgcgatc tgaggacggc
     4861 ggagacaatg actgagtgcc aatttccgcg cggaaatgct cgaagagctc gcgccacgac
     4921 gttttcgcca aactgggctt attcggcgga atgatgtcgg ccgcccctgc ggtggggcat
     4981 ttcaaaaatc tgttctcgat tggctttcgg gagcatgcgg gtctactgag gacgagcatc
     5041 ctgccggctc cgccctcgaa actatcctgg gctaagccgt ttgcgccgcc gggcttcctc
     5101 tcggccctag cggcttccat gctgccgcct ggcacgcctg gcacgctcgt gggcgctcgc
     5161 acagccaggc cattccgcga ccgttctcgc ggagcttccg ggacttcctc acgaaggcgg
     5221 ggctcaccca tcgccccaag ttgcgcttct gcgagcaatg gagccgccag cgacatagat
     5281 cggtcccccc tgtctgctgg tcttgttgcg tcgcttcgca acggtcacct cttcgttctg
     5341 cttcaacggc gtcgttaggt gcgccgagat gccgcgccga ttggagccga ctgcgacaaa
     5401 aatttgccag cattgacggt cgagctgacc gcctctcgct caggagcgac gcaatcctct
     5461 ccgcccgcga catgcgaatg cgtgcaagcg gcagcgattg gcggccccct ttccaaatgc
     5521 gacacgcaag ttgcgcgcct gggcctgcaa cccctccctg cctgggcgca gatcacaggg
     5581 gtgctgtgag ctagatgatg aaggcgaagc ctcaagctcg cccccggtat gccacacatc
     5641 ggccgggttg tcagccccag cgaccccttg cttgtcattt tagcctgtgg cggcagtcgt
     5701 cctcccggct gaggaagggg aattcgaggc cctgatcccc gaagcttgtc cgatttttgc
     5761 cgcaactcgc agccagtgtt gctgcagcaa gcgtggggga aatcatgaac agagtttggc
     5821 tggcttgtgt tctggctgtg gttttcgcga cagcaacagg tgctatcgcc atccggtcag
     5881 gcggacgcgc cgcacccacc acgaacctcg ccatcagcaa ggcagtcgca aaggtgaact
     5941 attatgacgt gcggggcacg acgctggagg agttgcgcca ggacgtgttt tcgcgcggac
     6001 cttacgacaa ggaggcgaac acccgcttcg ccggctgggc ggagtggcgc atccagtggt
     6061 ggttcgatcg ccgagaggtc gccgggggtt gcgctgtcgc caacgcttca accgagacgc
     6121 acatcgagta cacgctgccg cgctgggtgg acgaagacgc ggccccacct gagttacggg
     6181 caagctggaa gcggttcata gacgccctga cggagcacga ggagggtcac ggcaggctgg
     6241 cgcgtgagct cgccgagcaa atcgagttcg cgatcaggag cttgccgcct gagcctacct
     6301 gcgaggagct cgaccgcaag gtgaacgagc tcgccaaccg gctgatccgc gaggacaaga
     6361 cccaggaggc ttacgatcgg gaaaccgggc atggtgaaac gcagggtgct gcattcccga
     6421 gcatcctggt gcatgccact tcaaaaaccg cggctgtcag cgagtgacgc tctcgccctg
     6481 acgctcaacc tcgaggtctg gtcagcaggg gcgcatagat aacggcaaac cctgcgaagc
     6541 ccaccaccca agcggttgct gaaagatgca gcatcggctc gcgcgcgacg ccgaatccgg
     6601 ccacgatccg tgccaaggct gcgatgaaag tggcaatgta aattgccgta ataccccgcg
     6661 tcgcagtgag cggctgacct gtatgcccaa ggctggcgcg ggtcataacg gccatgatca
     6721 tcaggcccat tgcgcccgcc gtccaggcat gcagagcgcc cgacggcagg agcacttcgg
     6781 gtgcccagat actcgtggcg agcagcagaa atccgagtgg gatgaaagca taggccacgt
     6841 gcagcacgag cacgagctgc tctgccgtcg tgcgttcgcc ggcccaccgg ccgaggcgca
     6901 ccacgttcag caaacctgca aaaacggcga gaactgccgt cgcgtcggcc tccggcagca
     6961 cgatccagag ggccagcgcc gcaccgctga aagccatgac ggcggcgtcg aatcgcgaga
     7021 atgggacggg cagccgacca ggactccgcc gcaccagcca gttgcgcgtg aagctcggaa
     7081 tgatgcgacc gccgatcagc atgatcaaca ggatgatagc agctatgccg gcgcggatgc
     7141 cgtattccgc accgagcccg agcagtattt ccgcgtggaa cgtcacattc ccgaggaaca
     7201 gcacgcccag aacaatcagc accttcagat tgcgcgtgct ttttcccgcg atgatctcgc
     7261 gcgccaccac ggcagcgagg gtggcaagga agctcaaatc gatgacggcg gccagcggcg
     7321 caccgatcca acccgaagtg aaaacggcaa tccgcccggc aagccagagg agaaaaaggg
     7381 caagcagcgg ctttcccgtc acgggcagcc gtcctgtcca gttcgggacg gcggtaagca
     7441 agaagcccgc cacggccgcc ggtacgaacc cgtaaagcag cgcgtgcgcg tgccactcta
     7501 tcggtccgag agccgtcggc agcaccagtt gccctgcgag caccggcagc cagaggacca
     7561 tgacgagcgc ggcccaaatc gcggctgaca gaaagaaagg ccggaatcca aagctcagta
     7621 tcgccggtcc gcgatagctg cgaacttgct cagcagtggt tgccacggtc tattcccgcc
     7681 tcgaactctt gtggagccga atcgtaccca tcgaacgagg accgggctgg caccgttctg
     7741 agaggcgctc ggcggcagcc ctcggcgcgt tgaattcata tcacgccgcc gtaccttgct
     7801 ctgcgctatt cctggccagc ccgtactcga gcatcagctt gaggcccacc ttcgctggat
     7861 cctcagcctt tgcgagccgc cccattaaca tcagccaggt ttcgtcgtca gccaactgga
     7921 cgaaccgacc cacctcctcg cgcacgacgg tcgccaggtc gctgccgtct tcctcggcgg
     7981 ccgccttcag ccgcgccaga agggcgagat catcgagacc gatcaaggca tcgaggatga
     8041 cggcgtcatc ctgccaggcc gccagaagct ctcccagggt cattgcacag ggcctcccga
     8101 gacgcgcagg tcggccggag tcggtaagct gatgccgctc acctcgctcg ccgctgcgag
     8161 ccgggcgaca tactgggcga tcgccgtgcg ccggctccgc tcggtgagat acgcggcgat
     8221 gcgttcacgg acgagctcga acggcagctc gcggccatcg atgcgccggg tcaagtggat
     8281 gatgtggaac ccgtacctgc tctcgaccgg ctcggaggtc atttcccccg gtgcaagccg
     8341 acggagcgcc gcctcgaact ccggcgtcgt atcgcccgga ccgatctgcc caaggttccc
     8401 gccggatttg gccgacgggc aggccgaatg gatccgggcg aaatcggcaa acagctgcgg
     8461 gctttcgcga agatcagcga taagctgctc ggccttgttt cgcgccgcgc tccgtccctc
     8521 ttcatctttt ggatgcgcgg cgagaagaat gtgagaggcc tcgaacaggt ctgccgagcg
     8581 gaaacgccct ctgttctggc gataatagcg gcggcaggtt tcctcatccg gctcaggtgt
     8641 cgtgacctcc cgatcgatga gcccccgtat cagagcttcc tcgtccgtct ccctccggcc
     8701 ctctccatcc gtttccggct ccggctcgat gccaattcgc cgcgcctcct gcagcagcag
     8761 ctcacggacg acgagcgctc gggccgcagc ggtccacgcc gccagcggag tcggcgccgg
     8821 gtgatactgc gcctcacgcg aaatcatgtc gtgagggatg gtaacgccat tgactgcgat
     8881 tttcgtgcgt tgcgcattgg ccagcgcacc tctcgccgag cagcccatgg actcactccg
     8941 caggctcgat ggcagcagca cggccctccg atgccagtgg ccgccgtgtg cggacgacct
     9001 ggtagccgcg ccgcccgaga taccagaccg gcgcgctcca cacgtgcacc aggcgcgtga
     9061 acgggaagag caggaatatt gtcattccaa ggaacagatg cgctttgaaa acgggatgcg
     9121 catcggccac cagcatccat gtgtcggccc tgagcagcag cgtaccttgc gcccaattca
     9181 tgaagcgcac catctctccc ccgtcgaggt gccccatgga aaggggaatc gtcgccagcc
     9241 caaggaggag ctgcgccaag aggatcaaca gaatgccagt gtctgcaaac gatgacgtcg
     9301 cacggatgcg cgggtccatg agccgccgat gcgcgagcat cacaattccg accagacagg
     9361 ccaagccggc aacaccgccg acgacgatcg ccatgatctg tttggccgta tggctgatcc
     9421 cgatggtttc gaaaacccag ataggtgtca ataggccaac gaagtgaccg acgaaaatga
     9481 tcaaaatgcc gatgtgaaac aggttggacc ccaacatcag ctgccttctc ctgagcagct
     9541 ggctcgaacc ggatttccag gtgtactgat cgcgatcgaa gcgcagaatg ctgccaacca
     9601 cgaacacggt gaggcacacg taaggatacc agccgaagat gatataattg agtgtatccc
     9661 gcattttcta agctcccagt tcgcttcaac cctcgaaggg agagttaagc tcgggctgaa
     9721 cgcgtgtagg tgatcgtcgg gtggttggtt tgtccgtccc gcggggccac accggctgct
     9781 ggccgacgtg cggcacgaag tttgctgacc aggttctcgg caccgcaatc ggctgcggcg
     9841 ccaggcccga acctcacctc ctcatccacc cacgctgcat cgagcgcaga aagatcgttc
     9901 ggatcgtcat ccggcgccgc cagcaaggct tgaagcagct cgcccgcggg cgcggagctg
     9961 ctgagggcct gcagtgaaga gaataccgac gaatacggcg ccttgcgctt cttcagacgc
    10021 tgccggatcg cttcgagaat atgcgccgtc tcgccgagca gctcctttgc ttccgcctct
    10081 ggaatgacag acaggaattc gagaaacagg ggcagatagt cggggagctc attcgtggcg
    10141 agctccaggc catggcgctc gtaaagctgc ctgaggtcga ccatcgcctg cccgcgatcc
    10201 cggctttcac cgtgtacgtg ctcgaatagg ttgagcgaca gcgagcgggt ccgatcgaaa
    10261 agcaggatat agcgctcctg cagctcgtag agatccca
//
LOCUS       DASVDU010000013         5688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_744763, whole genome shotgun sequence.
ACCESSION   DASVDU010000013 DASVDU010000000
VERSION     DASVDU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744763"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..551)
                     /locus_tag="VF226_01055"
     CDS             complement(291..551)
                     /locus_tag="VF226_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEX7072606.1"
                     /translation="MSLAFGIAIYFVIWWMVLFAVLPFGVRTQEEAGEVVPGTPESAP
                     AAFRLLRVVVLNSLVAGVVFAGVWLVMKYHLIPLDQLTFLPG"
     gene            complement(599..1003)
                     /gene="mce"
                     /locus_tag="VF226_01060"
     CDS             complement(599..1003)
                     /gene="mce"
                     /locus_tag="VF226_01060"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX7072607.1"
                     /translation="MIGRLNHVAIAVRDLEAATAIYRDTLGAGVSEPLPQPAHGVTVV
                     FVTLPNTKIELLEPLGPDSPIAKFLERNADGGIHHVCYEVDDIIAARDRLKAAGARVL
                     GDGEPRIGAHGKPVLFLHPKDFLGTLVELEQA"
     gene            complement(1093..2802)
                     /locus_tag="VF226_01065"
     CDS             complement(1093..2802)
                     /locus_tag="VF226_01065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX7072608.1"
                     /translation="MTEARQAAPRRRSGEDEVVFMGLGGLGEIGLNVYLYGIGPEDAR
                     RWLMVDLGLTFANENEPGVDVVLPDLRFIEEERGSLVGIILTHAHEDHFGAVIDMWPR
                     LRAPIYATPFTAGMLRSKVAENGSLEELPINVVEMGSRFTVGPFDVELVTMAHSIPEP
                     SGVAIRTPYGIVFHTADWKLDHTPLVGDPTDEAKIRSLGIEGVRALICDSTNAFRAGR
                     SPSETEVAESLKKIIAGAKRRVAVTAFASNVARIRAIADAAKATRRHLVVAGRAMHRV
                     IQVAIDTGYLPEDFRYLDQDQFSYLEPDEVLILCTGSQGEPRAALARIAEGEHPDIRL
                     GQGDMVIFSARTIPGNEKAVTRVQNNLALLGVDVITDADALVHVTGHPRREELKEMYA
                     WLKPAVAVPMHGEARHLKEHAWLAKAAGVLDVHALRNGEMIRLAPDPVAIIDEAPVGR
                     LYRDGRLIVPSDGPVRDRRRLSFSGMVAIALAMGRRGELLGEPEIAIEGIPGETADGE
                     PMSEVVLTAVDGTLRSIPPAKRRDVELVRDAVRRGVRAAVDQVWGKKPIAKVLINVVD
                     TRR"
     gene            complement(2799..3599)
                     /locus_tag="VF226_01070"
     CDS             complement(2799..3599)
                     /locus_tag="VF226_01070"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7072609.1"
                     /translation="MPQAAPALPPAYRLLALDEIGSTNAEGMRLAALGETGPLWIWAG
                     TQTLGRGRSGRTWVSVPGNLYASLLIRLECPPGAVHQLSLLAGVAVHDAIHAAAARAG
                     VALSGLRLKWPNDVLIGEAKLAGILPESTTAPGRSGAVIAVVGMGLNLAGHPEGMGRP
                     VTHLAAHGLAITPADMLGFVAASVDDWLRRWDNGAGFAPVREAWLQRAGPPGERVVIN
                     TGRETVEGCFLGIDAQGALVLRDQGGAERRFTYGDVSLGVAAGVSDRQ"
     gene            complement(3609..5075)
                     /gene="nuoN"
                     /locus_tag="VF226_01075"
     CDS             complement(3609..5075)
                     /gene="nuoN"
                     /locus_tag="VF226_01075"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX7072610.1"
                     /translation="MTSMSEMQSWGPVLPELIVAIGSMLLLVYGVFRPDTDASGRTVG
                     WLAILVLLIAGLVIVLQPAGSTAYFEGAFVVDGFARFMKILVLGGAAVALLLSLDELS
                     DFRQMRFEYSVVMMLAAVGMMVMVSAGDLISLYLSFELQSLPSYVLAAYRRDQVRSSE
                     AGLKYFVLSALASGMLLYGASLVYGFTGSTQFLTILNVATGPEVSTNLGLIFGLVFVI
                     VGIAFKMSAVPFHMWTPDVYEGAPTPVTTFFASATKVAAMGLMVRFLLSAFPGMTPQW
                     QQIIVFLSIASMLLGAFGAIGQRNIKRMMAYSSIAHVGFALVGLAAASQEGIQGVVLY
                     MAIYVVMTVGSFACIVAMRRADGVTEDIGDLAGLAQHNLPMAFVFGALMFSLAGIPPL
                     AGFFAKFYVFLAAINAGLYGLAVIGVLASVISAFYYLRIVKIMFFDEPRDTFLPVRAK
                     TGFVMALSGALMLFSFIVPYVPAALITVANAAAGSFQY"
     gene            complement(5086..>5688)
                     /locus_tag="VF226_01080"
     CDS             complement(5086..>5688)
                     /locus_tag="VF226_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7072611.1"
                     /translation="KKLIAYSSVAHMGFVTMGIFARNQEGVQGSIFLMLSHGLVSGAL
                     FLCVGVIYDRMHTREIDAYGGLVNNMPKYAVAFMVFTMANVGLPGTSGFVGEFLTLIA
                     AFRTNTWVAFLATTGIILSAAYMLYLYRRIIFGALEKSSLKSILDMGPREVAVMAPLV
                     ILTILFGFYPATILDVTAVSVKNLVQNYQAAISTAAALAQ"
BASE COUNT         1110 a         1880 c         1812 g          886 t
ORIGIN      
        1 cattcagagc gatgacattg gttcgcgagt tggctatgct tgtggccgtc taggaacggg
       61 tccaaagccg gcgctaagat cggctatgtg cccaagtcta gggaagggag agagcgagcc
      121 cgccgtggca agtcgccctg cggccttgcg gcgaaccggc acatcctgtt ggcggatgag
      181 cgttcaagtc gccggaaaac ataaactggg gattatgagc gcgagagggc aaggcgaggg
      241 accttgctct ccgcttttat tttggctgga tgccagcagg cttagcttcg ttacccgggc
      301 aggaaggtca gctgatcgag cgggatcagg tgatatttca tcaccagcca cacgcccgcg
      361 aacacgaccc ccgccacgag actgttcagg acaacgaccc gcagcagtcg aaacgcagca
      421 ggtgcgctct caggcgtgcc ggggacgacc tcgccggcct cttcttgcgt gcgaacgccg
      481 aatggcagaa cggcaaacag caccatccac cagatgacga agtagatcgc gatcccgaaa
      541 gctaaactca ttccgtttcc tggccatttc cgggctttgt tctcgaccgc ccacgtcgtc
      601 aggcctgctc cagctccacc agcgtgccga ggaagtcctt ggggtgcaga aacagaaccg
      661 gcttcccgtg ggcgccgatc ctcggctcgc catcgcccag cacacgagcg cctgcggcct
      721 tcagccggtc ccgagcagcg atgatgtcgt cgacctcgta gcacacgtga tgaatgccgc
      781 cgtcggcatt gcgctcgaga aactttgcaa tcggcgagtc cggccccagg ggttccagca
      841 gctcgatctt cgtgttgggc agcgtcacga acaccaccgt cactccatgc gccggctgcg
      901 gcagcggctc tgagaccccg gcgcctaacg tgtcccggta gatggccgtc gccgcctcga
      961 gatccctgac cgcaatggcg acgtggttga gacgcccgat catgggttca gctcctctgc
     1021 aggaaaccgc tcacaccgtg gatcagcggg ccacagccga caactttcgc ccgaacagaa
     1081 tgcgatcgcc tgctaccgcc gagtatccac gacgtttatg aggaccttgg caatgggctt
     1141 tttgccccag acctgatcca ctgccgcgcg cacgccgcgc cgcaccgcgt cgcgcacaag
     1201 ctcgacgtcg cggcgcttgg ccggcgggat gctgcgcagc gtcccgtcga cggccgtcag
     1261 caccacttcc gacatgggct cgccgtcggc cgtctctcct ggaatgccct cgatcgcgat
     1321 ttccggctct ccaagcagct ccccgcgccg acccatggcc aaagcgatgg cgaccatgcc
     1381 gctgaacgac aggcgccgtc gatcccggac cggcccgtcc gacggcacga tcagccgccc
     1441 gtcgcgatag agccggccaa cgggcgcttc atcgatgatg gccaccggat cgggggcgag
     1501 cctgatcatc tcgccgttcc gaagggcatg gacgtcgagc acacccgccg ccttggcaag
     1561 ccacgcgtgc tccttgagat gccgcgcctc accgtgcatc ggcacagcga ccgcgggctt
     1621 cagccaggcg tacatttcct tcagctcttc ccgccgcgga tgccccgtca cgtgcacgag
     1681 ggcatcggcg tcggtgatga cgtccacgcc caagagcgcc agattgttct gcacccgagt
     1741 gacagccttc tcgttgcccg gaatcgtgcg tgccgagaag atgaccatgt ccccctgccc
     1801 gagccggatg tccgggtgct ccccttcggc gattcgggcc agggcagcgc ggggctcgcc
     1861 ctggctgccc gtgcacaaga tgagcacctc gtccggctcc aggtagctga actggtcctg
     1921 gtcgaggtag cggaagtcct cgggcagata gccggtatcg atcgccacct ggatgacccg
     1981 gtgcatcgcc cggcccgcca ccacaagatg gcggcgcgtc gctttcgctg cgtcggcgat
     2041 ggcgcggatc cgggccacat tcgaggcaaa cgcggtcacg gccacgcggc gctttgctcc
     2101 tgcgatgatc ttcttgagcg actcggcgac ctccgtttcc gaaggcgagc ggcccgcgcg
     2161 gaaggcattg gtcgagtcgc agatgagggc gcgcacgcct tcgatgccta agctgcggat
     2221 cttggcctcg tccgtcggat cgcccacgag cggcgtgtgg tcgagcttcc agtccgccgt
     2281 gtggaacacg atgccgtacg gggtacggat ggcgacgccc gatggctcgg ggatcgagtg
     2341 cgccatcgtc acgagctcga catcgaatgg gcccacggtg aagcgcgatc ccatttcgac
     2401 gacattgatg ggcagctcct ccagggagcc gttctcggcg accttggagc gcaacatgcc
     2461 cgcggtgaac ggcgtggcgt aaatgggtgc acggagccgc ggccacatgt cgatgaccgc
     2521 gccgaagtgg tcctcgtgcg catgggtcag gatgatcccg acgagcgaac cgcgctcctc
     2581 ttcgatgaag cgcagatcgg gaagcacgac atcgacgccc ggctcgttct cgttcgcgaa
     2641 ggtcagaccg aggtcgacca tgagccagcg gcgtgcgtct tcaggaccaa tgccgtaaag
     2701 gtagacgttg aggccgatct cgccgaggcc gccgaggccc atgaagacga cctcgtcctc
     2761 gccagacctg cgccggggag ctgcctgcct tgcttccgtc attgccgatc gctcacgccc
     2821 gcagccactc ccaacgacac gtccccatag gtgaaccggc gctctgcgcc accctgatca
     2881 cgtagaacga gtgctccctg tgcgtcgatc ccgagaaagc acccctccac agtctcccgc
     2941 cccgtgttga taacgacccg ctcaccaggg gggccagcgc gttgcagcca agcctcccgc
     3001 acgggcgcaa agccggcgcc gttgtcccac cgcctcagcc agtcgtcgac ggaggcggcg
     3061 acaaagccga gcatatctgc gggcgtgatc gcgaggccgt gggccgccag gtgcgtcacg
     3121 ggccggccca tgccctccgg gtggccggcg agattgaggc ccatgccgac cacggcgatc
     3181 acggctccgg accggcccgg cgctgtcgtg ctttcgggca atatgcccgc gagcttcgcc
     3241 tcgccgatga gcacatcgtt cggccatttc agcctaagtc cggaaagagc aacacctgcg
     3301 cgcgccgccg cggcgtgaat cgcatcgtgg acggcgaccc cggccagcag cgaaagctgg
     3361 tggacggccc caggcggaca ctccagccgg atgagcaggc tggcgtagag gttgcccgga
     3421 acggaaaccc aggtccggcc ggagcggccc cggccaaggg tctgcgtgcc ggcccagatc
     3481 cacagcgggc ccgtttcgcc caaggctgcg agccgcatgc cctcggcgtt ggtcgagccg
     3541 atctcgtcaa gtgcgaggag cctgtaagcg gggggaagtg ctggggccgc ctgcggcatc
     3601 ggctcccgtc agtactggaa gctgcctgcc gccgcattcg cgacggtgat cagcgcggct
     3661 ggcacatagg gcacgatgaa ggagaacagc atcagcgccc ccgagagcgc catcacgaac
     3721 cccgtcttgg cccgcaccgg caggaacgtg tccctcggct cgtcgaagaa catgatcttg
     3781 acgatgcgca ggtagtagaa ggcagaaatc acgctggcca gaacgccgat caccgcgagc
     3841 ccgtaaagcc ccgcattgat ggccgcgagg aagacgtaga acttggcgaa gaagccggcc
     3901 aggggaggaa taccggccag cgagaacatc agcgccccga agacgaacgc catcggcaga
     3961 ttgtgctggg cgagccccgc aagatcgccg atgtcctcgg tcaccccgtc cgcccggcgc
     4021 atcgccacaa tgcaggcaaa cgagccgacg gtcatgacga cgtagatcgc catgtagagg
     4081 accacgcctt ggatcccctc ctgcgatgcc gccgcaaggc cgacgagggc gaatccgacg
     4141 tgggcgatcg acgaataggc catcattcgc ttgatgttgc gctggccgat cgcgccgaac
     4201 gcaccgagca gcatggaggc gatcgaaagg aagacgatga tctgctgcca ctgcggcgtc
     4261 atgccgggga acgccgaaag gaggaagcgg accatcagcc ccatcgcggc gaccttcgtc
     4321 gccgaggcga agaaggtcgt caccggcgtc ggcgcgccct cgtagacgtc aggcgtccac
     4381 atgtggaacg gcacggccga catcttgaac gcgatgccga cgatcacgaa cacgagaccg
     4441 aagatcagcc cgaggttcgt cgacacctcc ggcccggtgg cgacattgag gatggtcagg
     4501 aactgcgtgg agcccgtgaa gccatagacc agcgacgcgc cataaagcag catgccggaa
     4561 gccagcgcgc tcaggacgaa gtacttgagg cccgcctcgc tcgagcggac ctggtcgcgg
     4621 cgataggctg ccagcacata cgatggcaag ctctgcagct cgaacgagag ataaaggctg
     4681 atgagatctc cggccgacac catgaccatc atgccgaccg cggccagcat catcaccacc
     4741 gagtactcga accgcatctg ccggaagtcg gagagctcgt cgagagacaa cagaagggcc
     4801 accgccgcgc cgcccagcac caggatcttc atgaaccggg cgaagccgtc gacgacgaag
     4861 gcgccttcga agtaggcggt gctgccggcg ggctgcagca cgatgacgag accggcgatc
     4921 agcaatacga ggatggcaag ccagcccacc gttcggccgg aggcatccgt atctggccgg
     4981 aagacgccat agaccagaag cagcatcgag ccgatcgcga cgataagctc gggaagcacc
     5041 gggccccagg attgcatttc gctcatcgaa gtcatctccc tgccgctatt gggcgagtgc
     5101 ggcagccgtg ctgatcgcag cctgatagtt ctggacgaga ttcttcaccg acacggcggt
     5161 gacgtcgagg atcgtcgctg gatagaagcc gaacaggatg gtgaggatga cgagcggcgc
     5221 catcacggcg acctcgcgcg gccccatatc cagaatggac ttgaggctcg atttctccag
     5281 cgcaccgaag atgatccgcc gatagagata gagcatgtag gccgccgaca agatgattcc
     5341 cgtcgtcgcc agaaaagcca cccaggtgtt cgtccggaag gcggcgatca gggtcaggaa
     5401 ctcgccgacg aagccgctgg tgcccggcag gccgacattg gccatggtga acaccatgaa
     5461 ggcgacggcg tatttcggca tgttgttgac cagcccgcca taggcgtcga tctcgcgcgt
     5521 gtgcatacgg tcgtagatga cgccgacgca gaggaacagc gcgcccgaga ccaggccatg
     5581 ggagagcatc aggaagatcg agccctgcac gccctcctgg ttcctcgcga aaatgcccat
     5641 tgtgacgaag cccatatgcg cgacggagga atacgcgatc agcttctt
//
LOCUS       DASVDU010000014        10651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1520337, whole genome shotgun sequence.
ACCESSION   DASVDU010000014 DASVDU010000000
VERSION     DASVDU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520337"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VF226_01085"
     CDS             complement(<1..620)
                     /locus_tag="VF226_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX7072612.1"
                     /translation="MVVIAVTAESADEPRVAASPDTVKRLANAGCNVKIESGAGLRSR
                     FGDDLYEAQGAHIARTREDTLSGADILLKVRRPSLDEVKALKPGAIVVAMLDPYSDRA
                     GLDALAATGAVLFSMEFMPRISRAQSMDVLSSQANLAGYKAVVNAAAMFEQAMPMMMT
                     AAGTVPAARVFVMGVGVAGLQAIATARRLGAVVTATDVRPATKEQVL"
     gene            complement(743..886)
                     /locus_tag="VF226_01090"
     CDS             complement(743..886)
                     /locus_tag="VF226_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX7072613.1"
                     /translation="MSIDTSGGNPAMDYHEHTRTYAAFVKGAKWLVGFIVLILVLMAI
                     TLL"
     gene            1182..1811
                     /locus_tag="VF226_01095"
     CDS             1182..1811
                     /locus_tag="VF226_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072614.1"
                     /translation="MRNYRLEFCAGFAAAVLMGGVAIAMIGVPPTQAFDKPEDERAVL
                     KACEKRLCEMIVKKEASGDDLVCALSKTWAKSSIKGGIEKKSLSWGFGDARCSVNLTA
                     KRDDIIGAVTRPEHALEFTPHTVKCEVERENEVTPINITLAPKVTFKAGKADQAWLNV
                     TEIEAPTAVKGAIWTAAQLEDTFGLFQSDMISEINEFIGEKCPKALAGA"
     gene            1935..3155
                     /locus_tag="VF226_01100"
     CDS             1935..3155
                     /locus_tag="VF226_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX7072615.1"
                     /translation="MRRGALRTIAAIALAGAFVPELALAATPGCQNTGSFDRWLANFR
                     REAVAAGISRATISTALDGMTLDRGVIARDRRQTFFAQSFLDVVQKLATAHRIRTGKQ
                     AMERHAAVFRRAEREYGVPAPVITAFWALESDFGAGMGKLAVLRSLATLAYDCRRGEK
                     FRGELMDALRIIDRGDLRPNQMIGSWAGELGQTQFLPSRYYKHAVDYDGDGRRDLLRS
                     PADVIGSTAAYIKHLGWRRGEPWLEEVRVTRDLPWKEADIAITHPRAQWAKWGITYAN
                     GRPLPRDGMPASLHLPMGRNGPAFLAYHNFTQVYLQWNQSLYYATTAAYLATRLDGAP
                     PMSKGRGPVEQFGLAETRELQRLLRRAGLYDQEIDGIMGAGTRAAVKQAQIRFGLPAD
                     AYPTSELLQRLRSM"
     gene            3255..4052
                     /locus_tag="VF226_01105"
     CDS             3255..4052
                     /locus_tag="VF226_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072616.1"
                     /translation="MLLNGKVAIVTGASQGIGRACAERLAREGAKVVIADINDELGPK
                     VALAITSAGGDAFFHACDVAERLDVHNLVAAALERYERVDILVNNAAVLDDKPFLELE
                     EAEFDRVIRTNIKGSFLCAQAVAKQLVKQIAEGGDKDNPGAIVNMSSVNAWFGLPDHV
                     AYTVSKGGISQLTKAMAVALAPYGIRVNAVGPGSIMTPMLEEVIKDKAFRTKVLSRTP
                     LGRLGRPEEIAAIVAWLASDEASYVTGTTVYADGGRMSLNYVMPVTE"
     gene            complement(4170..4637)
                     /locus_tag="VF226_01110"
     CDS             complement(4170..4637)
                     /locus_tag="VF226_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7072617.1"
                     /translation="MRPAVFTDYGLRVLMRLADAPDQPSTTARIAEEFQIPYNHLAKV
                     VQALARGGFVTTQRGAGGGIRLARPPESITLGEVVRYLEQRFALVECFRADGGKCLLT
                     PKCRLKPRLAAAREAFLDELDKTSLAECAYPGRDPDPTASVIRDEQGAGGLGS"
     gene            4802..5077
                     /locus_tag="VF226_01115"
     CDS             4802..5077
                     /locus_tag="VF226_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072618.1"
                     /translation="MSAQRIIRLSIAVLASGASMALSWPYWRNFEYWAESRSMWLVYF
                     ALGFVLAVYVFYAFLDSLGTLFEHDALERAKRANVKVRPAANREDAR"
     gene            5074..6039
                     /locus_tag="VF226_01120"
     CDS             5074..6039
                     /locus_tag="VF226_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7072619.1"
                     /translation="MSCAPGQPCAEGMKRRRAMAVLGVMAILFVALSVASFIDSRGQV
                     VPSDVKYANFDAVEGKRVFQSYNCMGCHTIVGNGGYFGPDLTKLYGKVGPAWLEAFLP
                     SAGSWPTKGAVKLQLQNEAVAADAGVADIEAYLEKYPAANERIARRGGHATAMPNLPL
                     TRSEVGRLIAFLKYTSEMNTEGWPPTPKVDGLTSPYAKPVPGAAQAAVSVTKEAVVAS
                     AKPVSAVAQGKMLAEENGCTACHATDRERLVGPGWGGIYGAEVQLADGSTVKRDDAYL
                     TQSILDPDARVVAGFEPGVMPSFAEMLDVDQVAAIVAYIRSLEGK"
     gene            6043..7725
                     /locus_tag="VF226_01125"
     CDS             6043..7725
                     /locus_tag="VF226_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7072620.1"
                     /translation="MIPIEYETQRLGLRFFLVMLVLFFFQTVFGLMLATQHVDPTLLA
                     GILNFNVIRSQHTNLAVLWVLSGFIGTILFVGPLLSKRELAAPWLIKFLFYALIAVVI
                     WNVITQVLAQQGIAGWWMGQPLLQEGLEYLEAGRIADVVILIGFGILCYVVLRTFPPR
                     SQWNEIHWGLGLGVVALTAVWIFGMFFVERLDLSEYFRWYVVHYWVEGVWEVIHISLV
                     GFLLVLMFKADVKSVGYAVFWGVSLVWLSGLLGNAHHYFWIGTPEFWQFWGALFSALE
                     PVPLIFCFWHIYLDAHQDGKPLENTPAFSFLLGSVVLEQVGAGILGFTMTFALTNVWS
                     HGTWVTVSHAHLALFGTFGMLGIAAAYYAIPLMRGVSNFDQRLGKLAFWLVFTGMLGL
                     GFAFAIGGTVQVFVYRTLGLDWFGGDVAPAMHFAKVMVPVFGIVFAIGVLLLVYDLLT
                     LGWRVPAEHDSAEIPAPSGWGRRLSSWETGLWLLAMWFFGGLITLGLLSFNLASVQQG
                     NPTLPYVLGGIGYPGLLLTTLLFVWRFLRSLDARRYVRAEPLAAASSLAGAA"
     gene            8154..8993
                     /locus_tag="VF226_01130"
     CDS             8154..8993
                     /locus_tag="VF226_01130"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7072621.1"
                     /translation="MIIESSEQVPRTRLFKRFPVPKNFGLAPQSRIQQGKRWPAPAGA
                     RRIKAFEVYRYDPETGENPRLDTFEVDLDQCGPMVLDALIWIKNNVDPTLAFRRSCRE
                     GICGSCAMNMDGTNWLACTRHISDLGDPGRIYPLASRTIIKDLVPDVTHLYAQYHMIE
                     PWLKTKTTEPNVERRQSPEERHELNGYYECILCFCCTSACPSHWWNSDRFLGPAALLQ
                     AWRWITDSRDEATEERLDNLEDPFRLYRCHTILNCTRTCPKGLNPAKAIAEIKKRMFE
                     RRS"
     gene            9038..9463
                     /gene="sdhC"
                     /locus_tag="VF226_01135"
     CDS             9038..9463
                     /gene="sdhC"
                     /locus_tag="VF226_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX7072622.1"
                     /translation="MARGTERPLASPSSQNRERVLPVSPNIQIYSPQLTSVLSFTHRV
                     TGVLLSLAAVGLVAWLIAGAVGPDAYAAVQGAISTWIGQIVLFVFTFAFFMHLCGGVR
                     HLVWDTVHAFELRQVYLGGWLAVAASIVLTIITWVVAYS"
     gene            9477..9878
                     /gene="sdhD"
                     /locus_tag="VF226_01140"
     CDS             9477..9878
                     /gene="sdhD"
                     /locus_tag="VF226_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX7072623.1"
                     /translation="MNRKDMRSPLARAIGLGSAKSGVEHWWAERLSAIALIPLTLWFI
                     ASIIAHTGSDYAAFTEWVRRPYVPILMIALLITLFYHAALGLQVIIEDYQHSWAKLAM
                     IIAAQFGCFILAIAGTVATLMVALGSDKALQ"
     gene            9915..10355
                     /locus_tag="VF226_01145"
     CDS             9915..10355
                     /locus_tag="VF226_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7072624.1"
                     /translation="MLVEKLLPAARNRLVTIRDDASLIDVAKLLQPGTDLIVVCDPRG
                     RLAGVITKTDVVAEIGRCRADNCDIQASSVMTRDVVVCHPDDWVEEVWSTMKQRGLKN
                     IPITDSDSRPLGVLNVKDALEVLLEEVESEDLLLREYVMNIGYQ"
BASE COUNT         1935 a         3264 c         3388 g         2064 t
ORIGIN      
        1 agcacctgct ccttggtggc cgggcggacg tctgtcgcgg tgactacagc gcccaagcgg
       61 cgcgcggtcg cgatggcctg cagccccgca acaccgaccc ccatgacgaa gacgcgcgcc
      121 gcgggcacgg taccggccgc ggtcatcatc atcggcatcg cctgctcgaa catggcagcg
      181 gcgttcacga ccgccttgta gccggcgaga ttggcctggc tcgacaggac atccatggat
      241 tgcgcgcgcg agatgcgcgg catgaactcc atcgagaaga gcacggcccc ggtcgccgcc
      301 agcgcatcga gtccggcgcg atccgagtag gggtcgagca tcgcgacgac gatggcgcca
      361 ggtttcagcg cctttacctc gtcgagcgac gggcgccgca ccttgaggag aatatcggcg
      421 ccggaaaggg tgtcctcgcg ggtgcgggcg atgtgcgcgc cctgcgcctc gtagaggtcg
      481 tcgccgaaac gcgaccggag cccggccccg ctctcgattt tgacgttgca gccggcgttc
      541 gccagcctct tcacggtatc gggtgaggcg gccacacgtg gctcgtctgc tgactccgcg
      601 gtaacagcga ttacgaccat cggggcaccg taacagctag gctgggaagc atcagggcgg
      661 gatcgccctt tcagggcggc aaatgagggt cgttgcgccg ctcaaggcgg cgcaacggaa
      721 aagttgcgcg agcggcacgc ggtcagagca gggtgatcgc catgagaacc aggatcagca
      781 cgatgaagcc gacaagccac ttggcaccct tgacgaacgc agcgtaggtc cgggtgtgct
      841 catggtagtc catggccggg ttcccgcccg aagtatcaat gctcactgca cccctccaca
      901 agtttgcctg acgggcatcc ggcggctcgt ttgcgccgcg ccggcgccca aaaattttca
      961 catagcacct atcccaggcg gcaacgggct tcaagtccgc agattgctgc atcccataga
     1021 ggattgtgat tggccggctg tatccggtct ggcgggcacg ttgtgcacag gcgcgagaat
     1081 cgtctcgatc ctgcccgatg ccggcgttaa gaaatcagca tgcggggaat ggcacaagta
     1141 gcgtgccgga gaagtgacca gttaccgtca gggggctttc gatgcggaac tacagactgg
     1201 agttttgcgc cggcttcgcg gcggccgtgc tcatgggagg tgttgcgatc gcgatgatcg
     1261 gggtccctcc aacgcaagcg ttcgacaagc cggaggatga gcgggcagtc ctgaaagcct
     1321 gcgaaaagcg gctttgcgaa atgatcgtca agaaggaagc cagcggggat gatctcgtct
     1381 gtgcgctgtc caagacgtgg gcaaagtcga gcatcaaggg cggcatcgag aagaaaagct
     1441 tgagctgggg cttcggtgat gcgcgctgca gcgtcaatct cactgccaag cgcgacgaca
     1501 tcattggggc ggtcaccagg ccggagcacg cactggagtt tacgccgcac accgtcaaat
     1561 gcgaggtcga gcgcgagaac gaagttacgc cgatcaacat cactttggcc ccgaaggtca
     1621 ccttcaaggc aggtaaggcg gatcaggcgt ggctaaatgt caccgagatc gaggccccca
     1681 ccgccgtgaa aggcgcgatc tggacggcgg cccagctcga ggacacgttc gggttgttcc
     1741 aatccgacat gatctcggaa atcaacgaat tcatcggcga aaagtgtccg aaggcgctgg
     1801 ctggagcgta agccctccat cacccgccag tggcccggcc aacgaggccg cgtcgtgcgg
     1861 gccggtaccc tctcgtgtca cattcgcctg atacactttg aacggtttta ccgattttgt
     1921 gaggaaggca gttgatgcgc aggggtgcct tacgcacgat tgcggccatt gccctggccg
     1981 gagctttcgt tcccgagcta gccttggcag ccacgccagg gtgccagaac accggcagtt
     2041 tcgatcggtg gctggccaat ttccggcgcg aggctgtggc cgccggcatc tcccgagcga
     2101 caatttctac agcactcgac ggcatgacgc tcgaccgcgg cgtcatcgcc cgcgatcggc
     2161 gccagacctt cttcgctcaa tccttcctcg acgtcgtgca gaagctcgcc accgcccacc
     2221 gcatccggac gggcaagcag gcgatggagc gtcatgctgc cgtcttcagg cgtgccgagc
     2281 gggaatacgg cgtgccggca ccggtgataa cggcgttttg ggcgctggag agtgatttcg
     2341 gcgccggcat gggtaagctg gccgtgctgc gctcgctggc gacgctggcc tatgactgcc
     2401 ggcgcggcga gaaattccgc ggcgagttga tggatgccct gcggatcatc gaccggggcg
     2461 atctccgtcc caaccagatg atcggctcct gggctggaga gctcggccag acgcaattcc
     2521 tgccgtcccg ctactacaag cacgccgtcg actatgacgg cgacggcagg cgcgatttgc
     2581 ttcgcagccc ggccgatgtg atcggctcga cggcggccta catcaaacat ctcggctgga
     2641 ggcgcggcga gccgtggctg gaggaggtgc gggtcacgag ggatctcccg tggaaagagg
     2701 cggacatcgc gatcacgcac ccgcgggcgc aatgggcgaa gtggggcatc acgtacgcca
     2761 acggcaggcc gttgccgcgg gacggcatgc cggcctccct gcacctgccg atgggccgga
     2821 acggacccgc cttcctcgcc taccacaact tcacgcaagt ttatctgcag tggaaccagt
     2881 ccctctacta cgccaccaca gcagcctacc tcgcgacccg gctcgacggc gcgccgccga
     2941 tgagcaaggg ccgcgggccc gtcgaacagt tcggtctggc cgagactcgt gaattgcaac
     3001 ggcttctgag gcgagcgggt ttatacgacc aggagatcga cggcatcatg ggcgccggca
     3061 cgcgcgcagc agtgaagcag gcccagattc ggttcgggtt gccggcggac gcttatccga
     3121 cgtcggagct gctccaacgg ctgcggagca tgtagagcga cgagcccctg ccgacgccgt
     3181 tgcggcccag ccgtcatgcg cagcttcgag cgctcgagcg cgcaggaatc atcaccggcc
     3241 cgaaagggga tgacatgttg ctcaacggca aggtcgcaat cgttactggt gccagccagg
     3301 gaataggccg cgcctgcgcc gagcgcttgg cgcgggaagg cgccaaggtg gtcatcgccg
     3361 acatcaatga cgagcttggc ccaaaggtcg ctctggcgat cacgtcggcc ggtggcgatg
     3421 ccttctttca tgcctgcgac gtcgccgagc ggctcgatgt gcacaatctg gttgctgccg
     3481 cgctcgagcg ctacgagcgg gtcgatatcc tggtcaacaa tgcagcggta ctcgacgaca
     3541 agccgttcct cgagctggag gaagccgagt tcgaccgggt gatccggacc aacatcaagg
     3601 gcagctttct ttgcgcccag gcggtggcca agcaactcgt caagcaaatc gccgagggcg
     3661 gggataagga caatccgggc gccatcgtca acatgagctc ggtgaacgcc tggttcgggt
     3721 tacccgacca cgtggcctac acggtctcca agggcggaat ttcgcagctc accaaggcca
     3781 tggcggttgc gctcgcaccc tacggcatcc gggtgaacgc ggtcggcccg ggctcgatca
     3841 tgacgccgat gctcgaagag gtgatcaagg acaaggcctt ccgcaccaag gtgctgtccc
     3901 gcacgccgct gggtcggctc ggcaggcctg aggagatcgc cgccatcgtg gcctggctag
     3961 cgagcgatga ggcgagctac gtgaccggaa cgacggtcta tgctgatggc gggcggatgt
     4021 cgctcaatta cgtgatgccg gtcacagagt agctgtctcg cggctcgatc cttcgaggct
     4081 gccgagtgga ggacatcgtc gctacgaccg gataaggggc aaagctctcc tagacgccag
     4141 tcgatcaaca ccgccgctga actccgatct tatgatccca gtcctccggc gccttgctcg
     4201 tcacgaatga cggacgcggt cggatccggg tcgcgtcccg gataggcgca ttcggccagg
     4261 ctggtcttgt cgagctcgtc gaggaatgcc tcgcgcgccg cagcaagcct cggcttcagc
     4321 cggcatttcg gcgtgagcaa gcactttccg ccatcggcgc ggaagcactc cacgagtgcg
     4381 aagcgctgct cgagataccg cacgacttcg ccgagcgtga tggattcggg cggccgtgcc
     4441 agccggatcc cgccgcccgc gccgcgttgc gtcgtcacga agccgccgcg ggcgagagcc
     4501 tgcacgacct tcgccaggtg attatagggg atctgaaatt cctccgcgat ccgcgctgtc
     4561 gtcgacggtt ggtccggtgc gtccgcaagc cgcatcaaaa ctcgcagtcc atagtcagtg
     4621 aaaacggcag ggcgcatgag cagcctttca aattggtgtt tacaatgcag atttgtccgt
     4681 ctaggaattg gcgaattgaa tacctgtttc caaggggcgt gtccaattgg tgcagcagca
     4741 gcaggtgggt cgtctgccac gtcgacgtga ccgctgacgg ctcactgcgc atggaggggc
     4801 aatgtcggcg caacgcatca tcagactttc gatagccgtt ctcgccagtg gagcgagcat
     4861 ggcactgtcc tggccgtatt ggcgcaattt cgaatactgg gccgaatccc gcagcatgtg
     4921 gctcgtttat tttgcgctcg gattcgtcct ggccgtctat gtcttctacg ccttcctcga
     4981 cagcctcggc acgctcttcg agcacgatgc gctggagaga gccaagcggg ctaatgtgaa
     5041 ggtcaggccc gccgcaaacc gggaggacgc gagatgagct gcgcccccgg tcagccctgc
     5101 gctgagggaa tgaagcgccg tcgcgcgatg gcggtcctcg gcgtgatggc catcctgttc
     5161 gtcgcgcttt cggtcgcgtc cttcatcgac tcgcgcgggc aagtggtccc ctcggacgtg
     5221 aagtacgcca acttcgacgc agtcgaaggc aagcgcgtgt tccagtccta caactgcatg
     5281 ggctgtcaca ccatcgtcgg caacggcggc tacttcgggc ccgatcttac gaagctctac
     5341 gggaaagttg gccccgcttg gctcgaggcg ttccttccat ctgcaggaag ctggccaacg
     5401 aagggggccg tgaagctgca attgcagaac gaggccgtcg cagccgatgc gggcgtcgcc
     5461 gacatcgagg cttacctcga gaaatacccg gccgcaaacg aacggattgc gcggcgcggc
     5521 ggccacgcca ccgcaatgcc caacctgccg ctcacgcgct ccgaagtggg ccggctgatc
     5581 gcgttcctga aatacacctc tgagatgaac acggaaggat ggccgccgac tccaaaggtt
     5641 gacgggctga catccccgta tgcgaagccg gttcctggcg cggcgcaggc ggcggtgtcg
     5701 gtgacgaagg aggcagtagt ggcgagtgcc aagcctgtgt ccgcagtcgc ccagggcaag
     5761 atgctggcgg aggaaaacgg ttgcaccgcc tgtcacgcta cggaccgcga gcgcctggtc
     5821 ggccccggct ggggcggaat ttacggcgcc gaggtccagc ttgcggacgg ttcgaccgtg
     5881 aagcgggacg acgcctatct cacccaatct atcctcgatc ccgacgccag ggtcgttgcc
     5941 ggcttcgagc ccggggtgat gccctcgttc gccgagatgc tcgatgtgga ccaggtcgcc
     6001 gccatcgttg cctacatccg tagtctggaa gggaaataag caatgatccc gatcgaatac
     6061 gagacccagc gcctcggcct gcggtttttc cttgtgatgc tggttctgtt cttcttccag
     6121 accgttttcg ggttgatgct ggcgacccag cacgtcgatc cgacgctgct cgcaggcatc
     6181 ttgaacttca acgtcatccg ttcgcagcac accaatctcg ccgtcctgtg ggtgctgtcc
     6241 gggttcatcg gcacgatcct gttcgtcggc ccgctgctgt cgaagcgcga gctggcagcg
     6301 ccgtggctca tcaagttcct cttctacgct ctcatcgccg tcgtgatctg gaacgtgatc
     6361 acgcaggtgc tggcgcagca gggcatcgcc ggctggtgga tgggccagcc tctgctgcag
     6421 gagggattgg aatacttgga agccggccgg attgccgacg tcgtcattct catcggcttc
     6481 ggcatcctgt gctacgtcgt gctcagaacc tttccgccga gatcgcaatg gaacgagatc
     6541 cattggggcc tcggcctcgg cgtcgtggcg ctgacggcgg tctggatctt tggcatgttc
     6601 ttcgtcgagc ggctggacct cagcgagtat ttccgctggt acgtcgtcca ctactgggtc
     6661 gagggcgtgt gggaggtcat ccacatctcg ctcgtcggct tcctgctcgt gctgatgttc
     6721 aaggccgacg tgaagtccgt gggctatgcc gtgttctggg gcgtctcgct cgtctggctg
     6781 tccggtctgc tgggcaatgc gcaccattac ttctggatcg gcacgcccga gttctggcag
     6841 ttctgggggg cgctgttcag cgcgctcgag ccggtgccgc tgatcttctg cttctggcac
     6901 atctatctcg acgcccatca ggatggaaag ccgctcgaga acacgccggc cttctccttc
     6961 cttctcggct cggtcgtgct cgagcaggtg ggcgcgggca tcctcggctt caccatgacc
     7021 tttgcgctca ccaacgtctg gtcgcacggg acgtgggtga cggtgagcca cgcgcacctt
     7081 gcgctcttcg gcacgttcgg catgctcggc atcgcggccg cctactatgc aattccgctc
     7141 atgcgcggcg tcagcaactt cgaccagagg ctcgggaagc tcgctttttg gctggtcttt
     7201 accggcatgc tcgggctcgg cttcgccttc gccatcggcg gcaccgtgca ggtgttcgtc
     7261 taccgcaccc tgggtctcga ctggttcggc ggcgacgtgg caccggcgat gcacttcgcc
     7321 aaggtcatgg tgcccgtctt cggcatcgtt ttcgcgatcg gcgttttgct gctggtctat
     7381 gacctgttga cgctgggatg gcgcgtgccc gcggagcacg attcggcaga gataccggcg
     7441 ccgtccgggt ggggccggcg gctgagcagt tgggaaacgg gcctgtggct gcttgcgatg
     7501 tggttcttcg gcggcctcat cacgctgggc ctgctcagct tcaatctggc gagcgtgcag
     7561 cagggcaacc cgacgttacc ctacgtactg ggcgggatcg ggtatccggg cctgctgctg
     7621 accacgctgc tcttcgtctg gcggttcctg cgctcgctgg acgcacgccg ctacgtcaga
     7681 gccgagccgc tcgcggccgc gtcgtcgctc gcaggcgcgg cctgatcggg ggagcggggg
     7741 tggcatcgat gcacgtcggc ggaatgatgt ggtgcctgcc ggcggtttac gggccgctcc
     7801 cgcgtccgat caacgtcgtg acgctgcgag cgactgcttc acgtcctgca aaagcctgaa
     7861 taaggcaaaa ctgatattga gtccgggatt cgctcgaaaa ctgcggtatc tcgcagtttc
     7921 aggcttcttg ggcttttcgc agatctgcgg cgaagcatgt cttgccgctt tagtgctcag
     7981 cgatgcctgc ggcattcctc cctgctttct cgctttgctt ggccgcagaa caggtgcctt
     8041 gtccatttgt cgggacctga tgcgctggaa cgtgccccag caataagtcg ttcactctat
     8101 cagaacccct cggccgtgcg gcggccgcct cgatccgctt tggaaggcga agaatgatca
     8161 tcgagtcctc agaacaggtt ccgcgtacga ggttgttcaa gcggtttcct gttccgaaga
     8221 atttcggctt ggctccgcag tctcgcatcc agcagggaaa gcgatggcca gccccggcag
     8281 gcgccagacg catcaaagcg ttcgaagtct atcgttatga cccggaaacc ggcgagaatc
     8341 cccggctcga caccttcgag gtcgatctcg atcaatgcgg gccgatggtg ctcgatgcgc
     8401 tgatctggat caagaacaac gtcgacccga cgctcgcctt ccggcgctct tgccgggagg
     8461 gtatctgcgg cagttgcgca atgaacatgg acggcacgaa ctggctcgcc tgcacgcggc
     8521 acatcagcga tctcggtgac ccggggcgca tctatccgct agccagcagg accatcatca
     8581 aggacctcgt gcccgacgtc acgcacctct acgcccaata ccacatgatc gagccctggc
     8641 ttaagaccaa gacgaccgag cccaatgtgg agcggcgcca gtcgccggaa gagcgccatg
     8701 agctgaacgg atattacgag tgcatcctct gcttctgctg cacgtcggcg tgtccgagcc
     8761 actggtggaa ttccgaccgc tttctagggc cggctgcgct tctgcaggct tggcgatgga
     8821 ttaccgacag ccgagacgaa gcgaccgaag aacggctcga caatctggag gacccgtttc
     8881 ggctctatcg ctgtcacacc atactgaact gcacgcgcac gtgtccgaaa ggattgaatc
     8941 cggcaaaggc gatcgccgaa atcaaaaaaa ggatgttcga gcgacggagc tgactggatc
     9001 gttgatccgg ctggaacgcc attcaagcgg aggaaagatg gccagaggaa ccgaacgccc
     9061 gcttgccagc ccttcttcgc aaaacaggga gcgggtcctt ccagtatctc ccaacatcca
     9121 gatctatagc ccgcagctca cctccgtcct gtcgttcacg catcgggtca cgggcgttct
     9181 cttgagcctt gcggcggtcg gcctcgtcgc gtggctgatc gcgggcgctg tggggcccga
     9241 cgcctacgcc gctgtgcaag gcgcgatttc cacctggatc gggcaaatcg tactgttcgt
     9301 cttcaccttc gcctttttca tgcatctgtg cggcggcgtc cggcatttgg tctgggatac
     9361 cgtccacgca ttcgagctcc ggcaggtcta cctcggaggc tggctggccg tcgccgcgag
     9421 catcgtactc acgatcatca catgggtggt cgcctattca taggacgcga aaagccatga
     9481 accggaagga catgcgatcc cctctcgccc gcgcgatcgg actgggttcg gcaaaatccg
     9541 gtgtagagca ttggtgggcg gagcgactat cggcaatcgc gctcattccg ctgacattat
     9601 ggttcatcgc ttcgatcatc gcacatacgg gaagcgacta cgccgccttt accgaatggg
     9661 tgcggaggcc gtacgtccct attctgatga tcgcactcct catcacgctg ttctatcacg
     9721 ccgctctagg attgcaggtc attatcgaag actatcagca ctcgtgggcg aagctcgcga
     9781 tgattattgc ggcccagttt ggctgcttca tcctcgctat tgccggaact gtcgcgacgc
     9841 tgatggtggc gcttggttct gataaggcac ttcaatgaaa agaacatcct ctaatcggca
     9901 ggaggatcag cccgatgctg gtcgagaaat tgctgcctgc agctcgcaac aggctcgtca
     9961 cgatcagaga tgatgcttcc ctgatcgacg tcgcgaagct tctgcagccc ggaaccgatc
    10021 tcatcgtcgt atgcgatccg agagggcgct tggcaggtgt gattacgaaa actgatgttg
    10081 tggctgagat cggccgctgc cgagcagata actgtgatat ccaggcgtcg tcggtaatga
    10141 cccgggatgt cgtggtgtgc catcccgatg attgggtgga ggaagtctgg tcgacgatga
    10201 agcagcgggg attgaagaac atcccgatca cggattcgga ttcccgacca ctgggcgtgc
    10261 tcaatgtgaa ggacgcactg gaggtccttc tcgaggaggt ggaaagcgaa gacttgctgc
    10321 tccgggagta cgtgatgaat atcgggtatc aatgacgcct gttggagccg gccgccactt
    10381 cattcttctc gggggtgcag ccgcgtaaga tgcagcgccg atcacgacgg cactcgccaa
    10441 atcggctttc cgctagttca tcgcgaccgt ctatgctgcc gtcccagtgc cgataacgat
    10501 cagatcgttt ccatgacgtt gatcctctga ggagtgagcg cgctcagcga tccaacaaga
    10561 ttttgcgcgc gtggcacacc tcacaaccgc ggcagcgcag agttgatctg gatcttgatt
    10621 tgttcacgag aaccgagcag caactaaggg c
//
LOCUS       DASVDU010000015         6046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_163351, whole genome shotgun sequence.
ACCESSION   DASVDU010000015 DASVDU010000000
VERSION     DASVDU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163351"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1426
                     /locus_tag="VF226_01150"
     CDS             290..1426
                     /locus_tag="VF226_01150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX7072625.1"
                     /translation="MTFTFVHTADWQIGRPFAGFPADKVPLLREARLDVIGRIAGVAR
                     QAGAQHVLVAGDVYDGPDVPDRDLRQSIDRLGREQDVIWHLLPGNHDAAHAGGVWERL
                     LHLGLPQNIRLHLEPRVEMIAEGVALLPAPLASRSVSNDPTSWMNEAEAPSARYRIGL
                     AHGSIQGFGGEDGEPAVPIAPNRAELARLDYLALGDWHGVTRVSERVWYSGTPEPDRF
                     PDNEPGFALVVRIGGEDQPVVVERHPTARYQWWKHAVTITGPETLAILEQTIAATVAH
                     QEHLLLKLALSGTASLSTWSDAEARLKALEERLFYLSVDSDALQVLPEEIELEEFGAG
                     DLRRVAELLSSTARGESSEEAPIASLALQKLYLLWQDARGEERA"
     gene            1423..4059
                     /locus_tag="VF226_01155"
     CDS             1423..4059
                     /locus_tag="VF226_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7072626.1"
                     /translation="MRIRAVRVRELGCFSEPVALEGLSGGLDVLAGPNELGKSTILKA
                     LQTLFTMRHSTQSKAIERLRPYGGGAPMVEADFEADGRLWRLRKQYLSERLAVLTDLT
                     AAGRVVARGDEAHARALELIGSGGEHRLGLLWLEQGMSLQPLSPKEGEREQLARVIER
                     EIAAASGGGHDLRTIRARAVQQRRQLVTDHKPPRPAGEYLKAITRRNATTEELEAARA
                     EARAAADRITRVTELRSRRAELTDPQVVAALRERVQKLEADCESAAAARQQLKLAETK
                     VQACESRVAEAQQAYALLANMLDESAKLEAAAAAAQPKVKELNEALEALTKAIDAARG
                     ERQTLQSALEAAQQRLRAREQLDRQREAAARLDDASRRLAEAQAAAQRAEEIRAELAS
                     FGVTEDGVAAAAREASSIEALTARIDAQLPKVRVAYQTGAAGKIRVGGRPVDDGAVLT
                     PSRAMTFEIEGIGTITVDPAVSESIEEDQADQEAHRSVLADLLASMGVADLSTARRRL
                     EERHRLERELAQAEDRIATRAPDGLERLSEEVERLTDIAEPRELDESDLPERSDIEAQ
                     IKGVETALRELEQRLEGLSRDQTAAREELAGINAAARAREERLTDLAKALPPVEERSN
                     RLLELENAVAAAQQAMNEAIRERSAWREKAPDDAAMSALESALAQARRHQQSAADEVA
                     RVERDLAVLEAQLDRDRQDGIAAKVKALEGELAAHEEQVRRFEREVAAINLLLETLST
                     VEQQSRERYFQPVLQRLDPYMNLVFPGAKLGLDRDFRVEAIDRGAVREQIGLLSDGTQ
                     EQIAVLARLAFARLLADAGRPTPLILDDALVYSDDDRIESLFDALRQAAEAHQVIVFT
                     CRSRTFAQLGGTRLSVAPWTVQ"
     gene            complement(4115..4579)
                     /locus_tag="VF226_01160"
     CDS             complement(4115..4579)
                     /locus_tag="VF226_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7072627.1"
                     /translation="MLPSFSNKKPEKDGAFGASGAGSSAGRVGRNGASVSIIGPDLVI
                     IGDLVSNGQVQVDGEVQGDIHGSHIVIGEGARVTGGIVSEEVVVRGTVEGSIRGRKVM
                     LQAESKVEGDIYHKSLAIEQGAYFEGKSRRLEDPLAGVQRPELPAEPAPAYD"
     gene            complement(4752..5228)
                     /locus_tag="VF226_01165"
     CDS             complement(4752..5228)
                     /locus_tag="VF226_01165"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7072628.1"
                     /translation="MVQEGDAAPDFELPTEDGSVRLSRLKGKPVVVYFYPKDDTSGCT
                     AEARDFNALRAEFADAGAEVIGISPDSVASHTKFSRKHGLQLKLAADEEKSAATAYGV
                     WVEKSMYGRKYMGVERSTFLVDPRGRIAKIWRKVKVPGHAEAVLKAVRELTSRPRP"
     gene            5456..>6046
                     /locus_tag="VF226_01170"
     CDS             5456..>6046
                     /locus_tag="VF226_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072629.1"
                     /translation="MTTRPRDITGPLRRTRQAGSMNPHVQRALVVGRALAKPCWTCGK
                     FLLPVVLLLVLAVGAFYVRILYGPISLKAIAGPVARSIAAEMPGLGVSIEDALVRLDE
                     QGELEFRLRNVRLLDELGSPVAVAPLAAVELSTEGLWSGRLSPDKVVLIEPRLLLTYS
                     QDGGLSFSFTRPAGSAGEGVPGGSDVQTLPTEQSSGE"
BASE COUNT         1052 a         1927 c         2010 g         1057 t
ORIGIN      
        1 gccgacgcct tgcgtccggc cgattcatcg tccatgcgcg acccatgcgc aactggtgtg
       61 atgatcgaga aacccgccta tcacggcata tgtcccgcgc ggattctcga tcattacatc
      121 ggtaagtgat gattgcagtg ccttctgatt cgcgtagctc gctttcgggt ctggctgcga
      181 gttccggcgc acttcagatc aggggcgctg cgtgcgaatt tccacagggt ccgggcaccc
      241 ggtcaatgcg gctcttgtca cgagaacata atgggaacat gatgctggca tgacgttcac
      301 gttcgtccat acggccgact ggcagatcgg caggcccttc gccggttttc ccgctgacaa
      361 ggtgcccttg ctgcgcgagg cacgcctcga tgtcatcgga cgcatcgctg gcgtggcgcg
      421 ccaagccggc gcgcagcatg tccttgtcgc gggtgacgtc tacgacgggc cggacgtgcc
      481 ggatcgggat cttcgacagt ccatcgaccg gctcggccgc gagcaggacg tgatctggca
      541 tctgctgcca ggcaatcatg atgcggcgca cgctggcggt gtctgggagc gcctccttca
      601 cctgggcctt ccgcagaaca tccgccttca cctggagccc cgggtcgaga tgatcgcgga
      661 gggcgttgcc ttgctgccgg cgccgctggc ctcgcgctcc gtctcgaacg atccgacgag
      721 ctggatgaac gaggcggagg ccccttcagc gcggtaccgg atcgggctgg cccacgggtc
      781 cattcagggg ttcggcggcg aggacggcga gccggcggtg ccgatcgcgc ccaatcgtgc
      841 ggaacttgct cgcctcgact atctcgctct cggcgattgg catggggtga cgcgcgtctc
      901 ggagcgcgtc tggtattcag gtacaccgga gccggaccgc tttccggaca acgagccggg
      961 gttcgctctc gtggtgcgga ttggcgggga agatcagccg gtcgtggtcg agcgccatcc
     1021 gacggcgcgt taccagtggt ggaaacatgc cgtcacgatc acaggtcccg agacgttggc
     1081 catactcgag cagaccatcg cggcgacagt ggctcaccag gagcacctgc tgctcaagct
     1141 cgcgctcagc ggcacggcct cgctgtcgac ctggtccgat gctgaggcgc ggctgaaagc
     1201 tctcgaagag aggctattct atctttcggt cgatagcgat gcgctgcagg tgctccccga
     1261 ggagatcgag ctggaggagt tcggagcagg cgacctgcgc cgggtcgcgg agcttctgag
     1321 ctcgacggca cgcggcgaat ctagcgaaga ggcccctatc gcctcgctcg ctttgcaaaa
     1381 gctctacctc ctctggcagg acgcgcgcgg ggaggagcgg gcatgagaat ccgtgcggtg
     1441 cgagtgcggg aactggggtg tttttccgag cccgttgcgc tcgaggggct ttccggaggg
     1501 ctcgacgtcc tggccggtcc taacgagctc ggcaaatcga ccatcctcaa agctctgcag
     1561 acgctcttca cgatgcggca ttccacacaa tcgaaggcga tcgaacggtt gcggccgtat
     1621 ggcggcggcg cgcccatggt ggaggccgac ttcgaggccg acggccggct atggcgatta
     1681 agaaagcagt acctgagcga gagactggcc gtgctgaccg atctgaccgc cgccggcagg
     1741 gtcgtcgcac gcggcgacga ggctcacgct cgggcgctcg agctgatcgg cagcggtgga
     1801 gagcatcgac tggggctgct ctggctggag cagggtatgt ccctgcaacc tttatcgcca
     1861 aaggagggcg aacgcgagca actcgcccgc gtgatcgagc gcgaaatcgc ggcggcaagc
     1921 ggtggcggtc acgacttgag aaccattcgg gcgagagcgg tgcagcagcg ccgtcagctc
     1981 gttaccgacc acaagccgcc ccgacctgct ggggaatacc tgaaagccat tacccgtcga
     2041 aacgcgacaa ccgaagagtt ggaggctgcg cgagctgagg cgcgtgccgc ggccgatcgg
     2101 atcacacgcg tcacagagct gcgttcgcgt cgtgcggagc tcaccgatcc ccaggtcgtt
     2161 gccgccctgc gtgagagggt tcagaagctc gaggcggatt gtgaatcggc agctgcggcc
     2221 cgccagcaat tgaagctggc ggagacaaag gtgcaggctt gcgagagccg cgtggccgaa
     2281 gcccagcaag cttatgcgct gcttgccaac atgctcgatg agagtgcgaa gctcgaggcg
     2341 gctgcagccg cggcgcagcc gaaagtgaaa gagctgaacg aagcgctgga ggcgctgacc
     2401 aaggcgatcg atgcggcacg aggggaacgt caaacgctgc agtcggccct cgaagcagca
     2461 cagcagcggc tccgggcgcg cgaacagctg gatcgccagc gtgaggccgc cgcccggctc
     2521 gacgacgctt cgcgccgcct tgcggaggca caagctgccg ctcagcgcgc ggaggagatc
     2581 cgggccgagt tggcttcgtt cggtgtgacg gaggatggtg ttgctgcggc cgcgcgggag
     2641 gcgagcagca tcgaagcgct gaccgcgcgg atcgacgcgc agctgccaaa ggtgcgcgtc
     2701 gcctaccaga cgggtgctgc gggaaagatc cgcgtcggcg gccgccccgt cgacgacggc
     2761 gctgtgctca cgccctcgcg cgccatgacc ttcgagatcg aaggcatcgg taccattacc
     2821 gtcgacccgg ccgtttcgga gagcatcgaa gaagatcagg ccgatcagga agcgcatcgc
     2881 tccgtcctcg ccgatctgct ggcgtccatg ggcgtagccg acctttcgac ggcacgccgg
     2941 cggctcgaag agcgccatcg gctggagcgc gagctggcgc aagccgagga ccgtatcgcc
     3001 acgcgtgctc ccgacggcct ggagcgactt agtgaggaag tggagcggct gacggacatc
     3061 gccgagcccc gcgagctgga cgagtctgac ctgcccgaac ggtccgacat cgaagcccaa
     3121 atcaagggcg tggagaccgc cctgcgcgag ttggagcagc gactggaggg tttgtctcga
     3181 gaccagactg ctgcccggga ggagctggcg ggcatcaatg ctgctgctcg tgcgcgtgag
     3241 gagcggctga cggatctggc gaaggctctt cctccggtcg aggagaggtc gaaccggctg
     3301 ttggagctgg agaacgccgt tgcggcagca cagcaggcaa tgaacgaggc cattcgcgag
     3361 cggagcgcgt ggcgggaaaa ggcgcccgat gatgcagcca tgagtgctct cgaatccgcg
     3421 ttggcacagg cgcgtcgcca tcagcagagt gcggccgatg aagtcgctcg ggtggaacgt
     3481 gatctggccg tcctcgaagc ccagcttgat cgggatcgcc aggacgggat cgcggccaag
     3541 gtgaaggcgc tcgaaggcga gctggcggcg cacgaagagc aggtccgacg gttcgagcgc
     3601 gaggttgccg cgatcaacct gctgctcgag acgttatcca cggtcgagca gcaatcgcgc
     3661 gaacgctatt tccaaccggt cctgcaacgg ctcgacccct acatgaacct ggtattcccg
     3721 ggcgccaaac ttgggctcga ccgagatttc agggtggagg cgatcgatcg cggtgccgtt
     3781 cgcgagcaga tcggattgct cagtgacgga acgcaggagc agattgcagt ccttgctcgc
     3841 ctggcgtttg cgcgattgct ggccgatgcc ggacggccaa ctccgctcat acttgacgac
     3901 gcgctcgttt actccgacga cgatcgcatc gagtcgctct tcgacgcact gcggcaggcc
     3961 gccgaggccc atcaggtcat cgtcttcaca tgccgctcca ggaccttcgc gcagcttggc
     4021 gggacgcggc tgagcgttgc cccgtggacg gtccagtgag acctggattg ggcggcgctc
     4081 cgagccggca ataacgccag ttcgtcgacg gagctcaatc gtaagcgggg gccggctcgg
     4141 caggcagctc cggcctctgg acccctgcca gcgggtcctc gagccggcgg gacttgccct
     4201 cgaaataggc gccctgctcg atggctaagg acttatgata gatgtcgcct tcgaccttgc
     4261 tctcggcctg cagcatcacc ttccggccgc ggatcgaacc ctcgaccgtt ccccgaacga
     4321 cgacctcctc ggagacgatt ccaccagtga ccctcgcgcc ctcgccgatc acgatgtggc
     4381 taccgtggat atcgccttgc acttcgccat cgacctggac ctgaccgttc gaaacaaggt
     4441 cgccgatgat gacgaggtcc ggacctatga tcgagacgct ggccccgtta cgcccgaccc
     4501 ggccggcaga agaaccggct ccggacgcac caaacgcgcc atctttctcc ggcttcttgt
     4561 tggaaaagct gggcaacatg gaaactacct ccactcgctc tcgtcactcc cccgggacgg
     4621 gcaagcgggc cgatcgcacc cccgcgtacg attgccactc ccccctgggc tgcaggttag
     4681 ccgatgcagc acacaatcat cctagcatgc gtccgaatgg tggtcaccgc cgctgaggaa
     4741 gcaaacaggc gtcatggtcg aggtcggctc gtcagctcgc ggacggcctt cagcacggcc
     4801 tcggcgtggc caggcacctt caccttgcgc cagatcttcg cgatcctccc tcgagggtcg
     4861 accagaaacg tcgaccgctc gacgcccatg tacttccggc catacatgga tttttccacc
     4921 cagacgccat acgcagtcgc tgccgacttc tcctcatccg ccgccagctt aagttgcagg
     4981 ccgtgcttcc tgctgaactt ggtgtgagac gccacgctgt ctggtgaaat cccgatgacc
     5041 tcagcgcccg cgtctgcgaa ctcggccctc agcgcattga agtcgcgcgc ttccgcggtg
     5101 cagccggagg tgtcgtcctt cggatagaag tagacgacca cgggctttcc cttcagcctg
     5161 ctcaggcgaa cgctgccatc ttctgtgggc agttcgaaat ctggtgctgc atcgccttcc
     5221 tgcaccatga gatgcctttc tttcgacgct ttgcgctctc acatcgttcg tggacgtaac
     5281 gtatcaggtc gcggggccgc aagggtgatt tctcggtcag agttggcgtg ccggcagctg
     5341 cgccagtcga gcagccagcc tgccgggcgt ccttgactgc gcctgcgttc tgccttgccg
     5401 acgcgtgagc cgcgtctgag cacttgacgg cacgttgacg gacagaggaa cgagcttgac
     5461 cacacggcca cgtgacatca ctggcccctt gcgccgtacg cggcaggcag gcagcatgaa
     5521 cccacatgtg cagcgcgcat tggttgtagg ccgggccctc gcaaagccgt gctggacctg
     5581 cggaaaattc ctccttcccg ttgtgcttct gctggtgctg gcggttggcg ccttttacgt
     5641 gcgcatcctc tacggcccga tctcgctcaa ggccattgcg ggacccgtcg cgcgcagcat
     5701 tgcagcagaa atgccggggc tcggcgtcag catcgaagat gccttggtgc gcctcgacga
     5761 gcagggcgag ctcgagttcc ggctgcgcaa cgtgcgcttg ctggatgaac tgggcagccc
     5821 ggtggcggtc gcgccgcttg cggccgtcga gcttagcacg gaagggttgt ggtcgggacg
     5881 cctgtcgccg gacaaagtcg tcctaatcga accgcgcctg ctcctcacct attcgcagga
     5941 cggggggctt tccttcagct tcacgcgtcc agccgggtcc gccggcgaag gcgtgcccgg
     6001 cggcagcgac gtgcagacgc tccctaccga gcagagctcc ggcgag
//
LOCUS       DASVDU010000016         3512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_770063, whole genome shotgun sequence.
ACCESSION   DASVDU010000016 DASVDU010000000
VERSION     DASVDU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770063"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VF226_01175"
     CDS             <1..1378
                     /locus_tag="VF226_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7072630.1"
                     /translation="GLDIDSFAPRLSFFWAIGMNFFMEIAKMRAARLLWANLIQTNFN
                     PKDKRSLSLRTHCQTSGWSLTAQDVFNNVTRTTIEAMAATQGHTQSLHTNALDEAIAL
                     PTDFSARIARNTQLVLQQESGTTRTVDPWGGSYYVERLTAELARRAMEHIREIESLGG
                     MAKAIAAGIPKLRIEEAAARTQARIDSGQQTIVGVNKYRVANEAEIEVLKIDNNAVRE
                     RQIAKLKQLKAERDPEAVRAALDALTEGARGNGNLLELSIAAARAKATVGEISEAMEK
                     VFGRHQETIRAVSGVYRAEASAMNKELARVQRMAEEFEKNDGRRPRILIAKIGQDGHD
                     RGQKVIASAFADLGFDVDIGPLFSTPTEVARQAVENDVHIVGVSSLAAAHLTLVPELK
                     AALEAEGRGDIMIVVGGVIPPSDYPAVYAAGARAIFGPGTNIPEAAADLIDKLNAELG
                     YGPRQAAE"
     gene            1410..1841
                     /locus_tag="VF226_01180"
     CDS             1410..1841
                     /locus_tag="VF226_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072631.1"
                     /translation="MSGRLIGGALLLILVTATAHAQSWYEPARGSKERRAIMDAMRPV
                     IEAKLDSPIEFVVHDLRVLGTWAFARVIPQRPGGGRIDLQRTMLRDFAEHLDGVRTEA
                     ILQRRNGRWYTVDHAIGATDVWFLAWCDRLPRGLLSDYCGP"
     gene            1952..2026
                     /locus_tag="VF226_01185"
     tRNA            1952..2026
                     /locus_tag="VF226_01185"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1984..1986,aa:Lys,seq:ttt)
     gene            2130..2393
                     /locus_tag="VF226_01190"
     CDS             2130..2393
                     /locus_tag="VF226_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072632.1"
                     /translation="MTWQHTVIGGETRPSDYCATDGDVPVGRIMKIEHGPAAGEWSWS
                     MYARVGWGKIWLTTHGRESTKQAAANKVAAIYAACLSDQNQTP"
     gene            complement(2395..2670)
                     /locus_tag="VF226_01195"
     CDS             complement(2395..2670)
                     /locus_tag="VF226_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072633.1"
                     /translation="MGEIDKQRVSAVRTLEEIGYVWDGIRWQPQREAAYWPEADTLHA
                     LLMDRAEVLSGCIEGTEQEDELRALADALEAYEARRWPEGKVPGGKG"
     gene            2785..3426
                     /locus_tag="VF226_01200"
     CDS             2785..3426
                     /locus_tag="VF226_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072634.1"
                     /translation="MTPTPHFIPPCIPSLAKSLPTGPGWLHEPKLDGWRIQLIKAGDR
                     LALFTRNGNDITARLLRLVEALGALPVRSCIIDGELVADEGDRIGNVFSINRAIGDGR
                     DDLCSVLAFDLLHCDGDDLRSLPLIDRKNALDALLATASIPALSYVAPHRDGARLFTT
                     VDELGLEGVVSKRAAAPYRSGRRGEWVKVKCQRWLEANRERWRIFQRPVEGAS"
BASE COUNT          655 a         1178 c         1090 g          589 t
ORIGIN      
        1 cgggctcgac atcgacagct tcgcgccgcg gctgtcgttc ttctgggcga tcggcatgaa
       61 cttcttcatg gagatcgcca agatgcgcgc cgcccgcctg ctgtgggcga acctcatcca
      121 gaccaacttc aacccgaagg ataagcgctc gctttcgctg cgcacccact gccagaccag
      181 cggctggagc ctcaccgccc aggacgtgtt caacaacgtc acccgtacga caatcgaggc
      241 gatggcggcc acacaagggc acacgcaatc cctacacacc aacgcgcttg atgaagccat
      301 cgcgctgcca accgacttct cggcccgcat cgcgcgcaat acgcagctgg tgctccagca
      361 ggagagcggc accacccgca cggtggaccc atggggcggc agctattacg tcgagcggct
      421 gacggccgag ctcgcccgcc gcgccatgga gcacatccgc gagatcgaga gcctgggcgg
      481 catggcgaag gcgatcgccg ccggcatccc gaagctgcgc atcgaggaag ccgccgcccg
      541 cacgcaggcg cgcatcgaca gcggccagca aaccatcgtc ggcgtcaaca aataccgcgt
      601 cgcgaacgag gccgagatcg aggtgctcaa gatcgacaac aatgccgtcc gcgagcgcca
      661 gatcgccaag ctgaagcagc tcaaggccga acgcgatccc gaggctgtcc gtgctgcgct
      721 cgatgcgctg accgaaggcg ctcgcggcaa cggtaacctg ctcgagctca gcattgcggc
      781 tgcccgcgcc aaggcgaccg tcggcgagat ttcggaggcg atggagaagg tcttcggccg
      841 gcatcaggaa accatccggg ccgtttccgg cgtctacaga gcggaggctt cggccatgaa
      901 caaggaactc gcccgcgtgc agcgcatggc cgaggagttc gagaagaacg acggccggcg
      961 gccgcgcatc ctcatcgcga agatcggtca ggacggccac gaccgtggcc agaaggtgat
     1021 cgccagcgcc tttgccgacc tcggttttga cgtcgacatc ggcccgctgt tctcgacgcc
     1081 caccgaggtg gcgcgtcagg ccgtcgagaa cgacgttcac atcgtcggcg tctcctcgtt
     1141 ggccgccgct cacctgacgc tggtgccgga gctcaaggct gcgctggagg ccgagggccg
     1201 cggcgacatc atgatcgtcg tcggcggcgt catccccccg tccgactacc cggccgtcta
     1261 cgcagccggc gcccgcgcca tcttcggccc cggcaccaac atccccgagg cagccgccga
     1321 tctcatcgac aagctcaacg ccgagctcgg ctacggtccg cggcaggcgg cggagtagag
     1381 ttcggcggtt tggggaggaa gggcgggata tgagcggaag gcttatcggc ggcgcgttgc
     1441 ttcttatact cgtgacagca acagcgcatg cgcaatcctg gtatgagcct gcccgcgggt
     1501 ccaaggagcg ccgggccatc atggatgcca tgcgcccggt gatcgaagcc aagctcgact
     1561 ccccgatcga gtttgtcgtg catgacttgc gcgtactcgg tacctgggcc tttgcgcggg
     1621 tcattccgca gcggcctggc ggcggtcgca tcgatctgca acggacaatg ctgcgcgact
     1681 ttgctgagca tctcgatggc gttcgcaccg aggcgatcct ccagcgccgc aacggccgct
     1741 ggtacaccgt cgatcacgcc atcggtgcta cggacgtctg gttcctcgcc tggtgtgacc
     1801 gcctgcccag aggcttgctt agcgattact gcggccccta agcagctagg ctcgagtcca
     1861 gcatcacggc cgcccgcatc tcgcggaaga ttcgctcact tcgccacgcg cccttgccat
     1921 cgaccccctc gacctgtatt cacgcgctcg tgggcccgta gctcagcggt agagcatctg
     1981 acttttaatc agagggtctc gggttcgaat cccgacgggc tcaccagaaa aatcaaagac
     2041 ttaaaagatc cgtaaaatcg accgcccaag aaaccgccca ggaaactcga tcgatctagc
     2101 cccgctctgc catgcttcag agcctaagat tgacctggca gcacaccgtc atcggtggcg
     2161 agacccgccc atccgactac tgcgcaacgg acggcgacgt tccggtcgga cgcatcatga
     2221 aaatcgagca cggtcccgca gcgggcgagt ggtcctggag catgtacgct cgcgtgggtt
     2281 gggggaaaat ctggctgacg acgcacgggc gagagagcac gaagcaggcc gctgccaaca
     2341 aggtggcagc gatttacgcg gcatgcctgt cagatcagaa ccagacgccc taggtcaccc
     2401 cttcccgccg ggcacctttc cctctggcca gcgtcgcgcc tcgtaggcct cgagcgcatc
     2461 ggcaagggcc cgcagctcat cctcttgctc ggtgccctcg atgcagcccg acagcacttc
     2521 ggcccgatcc atcagcaacg cgtgcagggt gtcggcttcc ggccaatacg ccgcctcacg
     2581 ctgcggctgc caccggatgc cgtcccaaac gtaacctatc tcttccaggg tccgcactgc
     2641 agagacgcgc tgcttgtcga tctcgcccat tccccacctc tcgcaagcgt caagttgcac
     2701 gagcatgtga tgcttgcgcg gcacggatat ggcgaacagt cgcgccacct tgcgccaaga
     2761 tcacgcttgc ggcagtggtg aggcgtgacg cctacgcccc atttcatccc gccctgcatt
     2821 cccagcctgg ccaagtcttt gccgacgggt cccggctggc tacatgagcc caagctcgac
     2881 ggctggcgta ttcagctcat caaggctggc gatcggctcg cgcttttcac gcggaacggc
     2941 aatgacatca ctgcgcggtt gctgcgcctg gttgaagcgt tgggcgcgct gcccgtccgt
     3001 tcgtgcatca ttgacggcga gctggtggca gatgagggcg acaggattgg caacgtgttc
     3061 agcatcaacc gggccatcgg cgacggccgt gacgacctat gctcggtgct ggcgttcgac
     3121 cttctgcatt gcgacggaga cgatctccgc agcctgccac tgatcgaccg gaagaacgcg
     3181 ctcgacgcgc tcctggctac cgcttcgatc ccagccctca gctacgtggc gccacaccgc
     3241 gacggcgccc gcttgttcac gacggtggac gagctcggcc ttgaaggcgt cgtgtcaaag
     3301 cgcgccgctg caccgtaccg atccggccgg cgcggtgaat gggtgaaggt gaagtgccag
     3361 cgctggctgg aggccaatcg ggaaaggtgg cggattttcc agaggcctgt ggaaggtgcg
     3421 agttgacgaa agcctgacga aagcctgcgc gccctctgat gtccgggctg atgaacagca
     3481 cagacacgtt gggggaagta atggtcgatt ta
//
LOCUS       DASVDU010000017        13842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1704353, whole genome shotgun sequence.
ACCESSION   DASVDU010000017 DASVDU010000000
VERSION     DASVDU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13842
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704353"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            416..3334
                     /locus_tag="VF226_01205"
     CDS             416..3334
                     /locus_tag="VF226_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEX7072635.1"
                     /translation="MEPHSLLLIVALPFLAGLATVLLPSDARTPATWLAGGTMAACLV
                     LTLSLYPTVGDGSALRQSFEWIPSLGLDFALRMDGFAWLFAFLVSGIGFLVVLYTRYY
                     ISPEDPVRRFYLFFLAFTGSMLGIVLSGNLVLLVVFWELTSIFSFLLIGYWHHNQAAR
                     DGARMALTITAIGGLALLVGVLMIGHIVGSYDLDVVLASGNAIRDHALYVPALVLILL
                     GAFTKSAQFPFHFWLPNAMAAPTPVSAFLHSATMVKAGVFLMARLWPVLSDTPEWFTI
                     VGVAGVSSFLLGAYLAMFQQDLKGLLAYSTISHLGLMTLLLSLGSPAGAVAAIFHMLN
                     HATFKASLFMAAGIIDHEAGTRDLRRLSGLFRYMPITGTLATVASAAMAGVPLLNGFL
                     SKEMFFAEAVETHADSWLDTAAPYAAVLGSALAVTYSIRLIHSIFLGAPPESFPREPH
                     EPPFWMRFPVTFLVFACLVVGIAPGLTVRPLLDVAVSAVLGAATPQYSLVVWHGFTVP
                     LVMSAVAMAGGALLYLVAGDYLERSERPLLFGRIEGQRIFERVNVAISWRWARLVEEI
                     FGTRRLQPQMRLLVAAALAAAAIPLFASGLGFAAYRSGAADAAFSALWAVGMVCAVAA
                     AYQAKYHRLAALILLGGTGLVTCVTFVWLSAPDLAATQLVVEIVTTVLILLGLRWLPK
                     RSEELFKQSGIFDRLRRLRDLSLAVVAGVGMTVISYAVMTRMPPDTIADFFVEKAYSE
                     GGGRNVVNVILVDFRGFDTLGEITVLGIVALTVFALLRRFRPAPDSIERPEQQLLQAH
                     DEEEQDRAVGTTAAEYLYVPSVIMQWLFPVVIVLAAYLFLRGHDAPGGGFAAGVAMAA
                     GFILQYMAAGTVWVEDRLRILPVLWIGGGLLLAALTGTASLLLGDPFLTSYFRYAELP
                     LVGKVPLASATMFDLGVFGLVVGATVLMLIAIAHQSIRRLRAARLEEERQLAEEQQWS
                     "
     gene            3325..3666
                     /locus_tag="VF226_01210"
     CDS             3325..3666
                     /locus_tag="VF226_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX7072636.1"
                     /translation="MELTLAIAIGVLTGSGVWLVLRPRTYQLVIGLSLLSYAVNLFIF
                     SMGRLRLGAAPVLGADGTGNPADYADPVPQALVLTAIVIGFATTALLLVVLIAARGFT
                     GSDHVDGRESR"
     gene            3663..5303
                     /locus_tag="VF226_01215"
     CDS             3663..5303
                     /locus_tag="VF226_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX7072637.1"
                     /translation="MMEWAKHLLVVPILLPLVAGAVLLLIDEVRHTVKAAISLASTLL
                     YLLAAVTLMRIADGVPVGSGALSWSYAVANWPAPFAIVLVLDRLSALMLVLTSVLALA
                     SLVFSMARWHRAGPHFHTLFQFLLMGLGGAFLTGDLFNLFVFFEVTLAASYGLLLHGS
                     GAFRVRSGLHYISINLAASLLFLIGVSLIYGVTGTLNMADLALRAPSVPAEDRVLLQA
                     GAAVLGVAFLVKAGMWPLCFWLPSAYTAAAAPVGALFVALTKVGVYVLLRLTLLLFGS
                     DAGELTGFGGPALLWGGIATLAFATSGALASQAMGRLAGYSVLISSGTLLAAVGMGDA
                     TVVSGALFYLVSSTFSTAAFFMLIELVERAREPGADLLAVTVEAYGEEEDEAEEERED
                     EEVGVVLPGALAVLGVCFGCVALLLAGLPPLSGFIAKLAMLTGMLNLEELGRPADIPA
                     RTWVLVTLIILSGLAVLVAMMRSGIRIFWAPLETIIPRVLVVEVAPVVALLVLCLILT
                     VQGGAMISYTEAAARSLHDPSGYIQGVLGTLETARGER"
     gene            5300..5785
                     /locus_tag="VF226_01220"
     CDS             5300..5785
                     /locus_tag="VF226_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX7072638.1"
                     /translation="MSRVLPYPVLTAALLLMWMLLTSFSPGQFLLGAVIAAGASKAMA
                     ALHPSKPRVRRWQAIPRLAGIVMLDILRSNAAVASIILQGRHRDRVAGFVAIPLELRD
                     RTGLAVLACIITSTPGTAWIEYSVETGVLIIHVLDLVDEEEWVALIKNRYEKLLMEIF
                     E"
     gene            5782..6063
                     /locus_tag="VF226_01225"
     CDS             5782..6063
                     /locus_tag="VF226_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEX7072639.1"
                     /translation="MTADILLWSVTVAQAMLVIAMGCNTYRLIRGPRAQDRVLSFDAM
                     YVIAMLLVLTFGIRTGNAFYFEAALIIAALGFVSSVALAKFLMRGEVIE"
     gene            6060..6461
                     /gene="mnhG"
                     /locus_tag="VF226_01230"
     CDS             6060..6461
                     /gene="mnhG"
                     /locus_tag="VF226_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX7072640.1"
                     /translation="MTHAADLPAWAALLTALLVLLGAGLTLVGAIGTLRLGSFFERVH
                     APTLGTSWGTGAIVLASIVCFSVLGSRVVLHEFLIGVFITVTTPVTLTLLVRAALYRE
                     RAEGNTDVPARSVLRAPQSLNRREPGGDTAR"
     gene            6842..7324
                     /locus_tag="VF226_01235"
     CDS             6842..7324
                     /locus_tag="VF226_01235"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX7072641.1"
                     /translation="MSAYEIRSSTARNNPAHYTPRVTIAQLFLRAVQYAVQRWQRRRA
                     IAELESLSDRELKDIGIARHDIPRAVDGLIRSRDGSSEGPRRREPAGPPSGSERQPEE
                     QRKGSNLGRSGVLRLAYGGNDGHDNENDHRSGHVRADVRGFRNDVTTRSGVARDTVSA
                     "
     gene            7596..8333
                     /locus_tag="VF226_01240"
     CDS             7596..8333
                     /locus_tag="VF226_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7072642.1"
                     /translation="MVLFSGRRACDIGGSVQADSPSGRPPGMVTGAAVNGDRRAHAFS
                     DKAHRPDAAPVVAPHPASAREAAFQAEPKTKLSELLGAQDILFETRSGRKCTSLGAIA
                     VRLGEKVGRSQGSVLAALLRRERLGSTAIGNGTAMPHARLDGIRAPAAVLARLQRPLQ
                     FGTSDDDRVDLLLGLVWPKSDPRGFVAGLHHGWRLMRRSGLADLFRQSRTPAEALALI
                     KSFEEEKSDARGHIGPAAPAMTGPGGS"
     gene            8466..9215
                     /gene="map"
                     /locus_tag="VF226_01245"
     CDS             8466..9215
                     /gene="map"
                     /locus_tag="VF226_01245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7072643.1"
                     /translation="MTISNDNEFGRLREIGRIVANVLEAMGAAIEPGMTTAELDELGR
                     RLLKQEGARPAPELCYGFPGATCISVNEEIAHGIPGGRRIAAGDLVNIDVSAEKDGVF
                     ADTGASFTVPPVSRAIERLCRDGRRATWTGIRQIAPDRPLANIGFAIGAFAHKNRYTL
                     VQNLASHGVGRSLHEEPSEVATWPDPTERRKMHEGLVFTVEPFLSMGAKWATGGDDPW
                     TLFSEPHAPTVQYEHTVVVGRNGPVVVTLLG"
     gene            complement(9354..9947)
                     /locus_tag="VF226_01250"
     CDS             complement(9354..9947)
                     /locus_tag="VF226_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEX7072644.1"
                     /translation="MKILHVDTSITGRASVSRALSAEIVASLRRLQPEAEVTVRDLAA
                     QPIGHLSDAHLLADQGAVPGEAAIQDDLAAGQAALEEFLAADIVVIGAPMYNFAIPSQ
                     LKAWIDRLAVRGVTFRYTDDGPEGLAKGKKVIIASSRGGFPSEALDHQETYLRTIFGF
                     FGITDITTVRAEGVAFGEEAKASAIKGARCEIASLAA"
     gene            10049..10960
                     /locus_tag="VF226_01255"
     CDS             10049..10960
                     /locus_tag="VF226_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7072645.1"
                     /translation="MEFGVSLDQLRVFATVVEEGSFSAAARRLNRSQASITYAVQKLE
                     SAVGTLLFDRSGYRPTLTDAGRTLLPRARRVVETSAGFERQARALREGIEAELTLVVD
                     AMFPMPLLFAALSAFDRQFPTVQTRLYVESLGAAVKTVLDGNADLGIVIDFANVSDRL
                     ASAAMNEIELVPVAAPTHPLALMQAQSDKALSDEELRDHLQLVLTDRSELTKGKDTGV
                     VSVRTWRLADLGAKHAMLLAGMGWGNMPLHMISSDLAAGRLVELEIARWPKTRRSTVA
                     TVIVHRLDRPPGPAGAWLLSRLAGDER"
     gene            complement(11439..12725)
                     /locus_tag="VF226_01260"
     CDS             complement(11439..12725)
                     /locus_tag="VF226_01260"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7072646.1"
                     /translation="MTLAMIALALLLLIGVPIAFAVGIMSVGYMFAVGGADFLLLVPQ
                     RMFAGLNVFTIMAIPFFFLAAELMTASKILDDLVEFADAIVGRFRGGLAHVNVLGSAF
                     FAGISGSAIADVAGLGAIEIRAMEAAGYDRRFATALTVASSLIGPIIPPSIIILLYAS
                     IMQVSVATMFLAAVLPGILLTVMLMIVVAFMARKYDFPKRNTRYSPVEFLSITVRASS
                     ALLMPLIIMGGILSGIFTATEAAAVAAFYALVIGLFAKRTLKLADIYQCLLRSGIMTS
                     VIFLVLATASIFGWIIGLERVPETMAKALVGITADPILLLTLIVGLLLIVGMFMDVGA
                     ALIVMGPILAPTAISAGIDPVHFGIVMSLSLVVGLNTPPVGPCLFAAASISRLKIEEI
                     SMALIPFYVVQVVFLAIIMLIPSISLWLPQWLGYLK"
     gene            complement(12751..13269)
                     /locus_tag="VF226_01265"
     CDS             complement(12751..13269)
                     /locus_tag="VF226_01265"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7072647.1"
                     /translation="MKTFMRVADLLLAIAKILTVILMAGMTISILLGVFFRFVVSDPL
                     SWPPEAARFMMVAITMLAGSVAVRQLDHVGVTFLVDSVPPRLRFAAYIVGLVLTALFL
                     ALLVVYSFRLTFEAGPLQRAPTLGIPMTVVFFSMPLGAFMMLVQLGASLSEGIKRARS
                     GNSPFEAGETVA"
     gene            complement(13327..>13842)
                     /gene="dctP"
                     /locus_tag="VF226_01270"
     CDS             complement(13327..>13842)
                     /gene="dctP"
                     /locus_tag="VF226_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7072648.1"
                     /translation="RTMEIPAHQKMVTSLGASPTAIPWLEVYGALQTGVVDGAELPII
                     GTLQQNLQEVLKYVTIDQHVYSLALIVISEKWYQSLPDDLREAVIIAGKQAQVAGRGL
                     SQSLVNDVIDEFRKQDVVVTVASPEEQEAFRKTAQPPVVDWMVEQFGKAYVDEFFDAI
                     ESAEEELGMTN"
BASE COUNT         2432 a         4328 c         4353 g         2729 t
ORIGIN      
        1 ctcgtgggga tgccggtctg gcaatgtcgg tgattggatg cgcgatcggg tcgcgtcgac
       61 ccgcggactt tccggatttt ccgcccctcg gcccggggcg catgacagcc gagccggggg
      121 ttacgatccc gcgatggggc aaacccggag aatggtattc tggcgtgtga tggctgcgat
      181 agtcatagct ggatagttgg cggttggaac cgggaaagtc aaggcacggg cgggacgggg
      241 tagacccgca tcggctttcg acgcgcgtgg caacgaacat gccgagcgcg gcgcggcgcg
      301 gcagagccgt gcgtctgccg acatgtttgc atgcgagcac ctgcttgccg ccgttggcag
      361 gacactgccg atgccttaaa ccaccgtaag atgccatcca gccgcaagga gcgcgatgga
      421 gccgcactca ctacttctga tcgtcgcgct gcctttcctg gcaggtctcg cgaccgtcct
      481 gcttccgtcg gacgcacgga cccccgccac ctggctggcg ggcggcacga tggcggcttg
      541 cctggtcttg accctctcgc tttatccgac ggtcggcgac ggaagcgctc tccggcaaag
      601 tttcgagtgg attccctcgc tcgggctcga cttcgccctc aggatggacg ggtttgcttg
      661 gctcttcgcc tttctcgtca gcggcatcgg gttcctcgtc gtcctttata cgcggtacta
      721 catctcgccc gaggacccgg ttcgtcgttt ctatctcttc ttcctggcgt tcacgggctc
      781 gatgctaggc atcgtgctgt cgggcaacct ggtcctgctg gtggtcttct gggaactcac
      841 cagcatcttt tccttcctgc tgatcggtta ctggcatcat aaccaggcag cacgcgacgg
      901 cgcgcgcatg gcgctgacga taacggcgat cgggggcctt gcccttctgg tgggcgttct
      961 catgatcggc catatcgtag gcagctacga tctcgatgtt gtgctggcct cgggcaacgc
     1021 aatccgtgac cacgccctct acgtgccggc gctggtgctg atcctgctcg gcgcgttcac
     1081 caagagcgca cagttcccct ttcacttctg gctgcccaac gcaatggccg cacccacgcc
     1141 ggtgtctgcg tttctgcatt cggcgaccat ggtcaaggcc ggggttttcc tcatggcacg
     1201 cctttggccc gtgctctcgg acacgccgga atggttcacc atagtgggag tggcgggagt
     1261 gtcctcgttc ctgctgggcg cctatctcgc gatgttccag caggacctga aagggctgct
     1321 ggcctattcc acgatcagcc atctggggct gatgacactg ctgttgagcc tcggcagccc
     1381 ggccggagcg gtggccgcga tattccacat gctcaaccac gcgaccttca aggcgtcgct
     1441 gttcatggca gccggcatta tcgatcatga ggcaggcacg cgagacctgc ggcggctgag
     1501 cggcctattc cgctacatgc cgatcacggg cacgctggcc acggtcgcaa gtgcggccat
     1561 ggcgggcgtg ccgctgctga acggctttct gtcgaaggag atgttcttcg cggaagccgt
     1621 cgaaacccat gccgactcct ggctggatac agccgccccc tatgctgccg tgctcggaag
     1681 cgcgctggcg gtgacctatt ccatccggct catccactcc atcttcctcg gcgcgccgcc
     1741 cgaaagcttt ccgcgcgagc cgcatgagcc gccgttctgg atgcggttcc cggtcacgtt
     1801 cctggtgttt gcctgtctcg ttgtcggcat cgccccgggt ctgaccgtca ggcccttgct
     1861 ggacgtcgcc gtctcggccg tgctcggcgc ggcgacaccc caatacagcc tggtggtctg
     1921 gcacgggttc accgtccccc tggtaatgag cgcggtggcc atggcgggcg gggcgctgct
     1981 ctatctggtg gcaggggact acctggaacg ttccgaaagg ccactgcttt tcgggcggat
     2041 agaaggccag cgcatcttcg agcgggtcaa cgtcgcgatc tcatggcgat gggcacggct
     2101 cgtcgaggag attttcggta cgcggcgcct gcagccgcag atgcgcctgc tcgtggcggc
     2161 cgcgcttgcc gccgccgcaa ttccgctttt cgcgtcaggg ctcggattcg ccgcataccg
     2221 cagcggagcg gcggatgccg ctttttccgc cctttgggca gtcggcatgg tctgcgccgt
     2281 cgcagcggcg taccaagcca agtaccaccg cctggcagcg cttatccttc ttggcggaac
     2341 cgggctcgtg acctgcgtca ctttcgtctg gctctcggca ccggaccttg ctgcgaccca
     2401 attggtggtc gagatcgtca caaccgttct catactgctc gggctaaggt ggctgccgaa
     2461 acgctcggaa gagctgttca agcagagcgg cattttcgac cgcctgcgcc gcttgcgcga
     2521 cctttcgctg gccgtggtgg ccggtgtcgg catgacagtg atttcctacg ccgtcatgac
     2581 gcgcatgccg cccgatacca tcgcagactt cttcgttgag aaggcctaca gcgagggcgg
     2641 cgggcgcaac gtcgtcaacg tgatcctcgt cgatttccgc ggtttcgaca cgctgggcga
     2701 gatcacggtc ctcggcatcg tggcgctcac cgttttcgca ctgctgcggc gcttccgccc
     2761 cgcgccggac agcatcgaac ggccggagca gcagctcctc caggcccatg acgaagagga
     2821 gcaggacagg gcggtcggca cgacggccgc cgaatacctg tacgtgccgt cggtgatcat
     2881 gcaatggctg tttcccgtgg tcatcgtcct ggccgcctat ctgttcctgc gcggccacga
     2941 tgcgccgggc ggaggctttg ccgccggggt ggccatggcc gcgggcttca tcctccagta
     3001 catggcggca gggacggtct gggtagagga ccggctgcgc atcctgccgg tgctgtggat
     3061 cggcggcggc ctgcttctgg ctgccttgac cgggacggca tcgctgctgc tcggcgatcc
     3121 gtttctcacc tcgtacttcc ggtacgcgga actgccgttg gtgggcaagg tcccgctggc
     3181 gagcgcgacg atgttcgacc tcggcgtgtt cggccttgtc gtcggcgcca cggtgctgat
     3241 gctgatcgcc atcgctcatc agtcgatccg ccggctgcgc gcagcacgac tcgaggagga
     3301 acgccagctt gcggaggagc agcaatggag ctgacgcttg cgatcgccat cggcgtgctc
     3361 accggatcgg gcgtgtggct tgtcctgcgt ccacgcacct accagctggt cattggcctt
     3421 tcgctgctct cctatgcggt aaacctcttc atcttcagca tgggccgctt gcgcctcggc
     3481 gcagcccccg tgctgggcgc ggatggcacc ggcaacccgg cggactatgc ggatccggtt
     3541 ccgcaggctc tggtgctcac cgccatcgtg atcggcttcg ccacgacagc gctgctcctg
     3601 gtggtgctga ttgccgcccg cgggtttacg ggcagcgacc atgtcgacgg gcgggagtcg
     3661 cgctgatgga gtgggcgaag cacctcctgg tggttccgat cctgctgccg ctcgtggccg
     3721 gcgcggtgct cctcctcatc gacgaggtgc gccatacggt gaaggcggcg atcagtctgg
     3781 cttcgaccct gctgtacctt ctcgcggccg taacgctcat gcgcatcgcc gacggggttc
     3841 cggtcggaag cggcgccttg tcgtggtcct atgccgtcgc gaactggccc gcccccttcg
     3901 ccatcgtgct cgtgctcgac cgtctgtcgg cgctgatgct ggtccttacc tccgtcctgg
     3961 cgctcgcctc gctggtcttc tcgatggccc gctggcaccg cgcgggcccg cacttccaca
     4021 cactgttcca gttcctgctc atggggctcg gcggcgcatt cctgaccggg gatctcttca
     4081 acctgttcgt cttcttcgag gtgacgcttg ctgcatccta cggcctgctg ctgcacggtt
     4141 cgggcgcgtt ccgggtacgc tccgggctgc actacatatc gatcaacctt gcggcctccc
     4201 ttctcttcct catcggcgtg agtctgatct acggggtcac gggcaccctc aatatggcgg
     4261 atctcgccct gcgcgcgccg tcagtgccgg ccgaagaccg ggtgctgctg caggcgggcg
     4321 cggcagtcct gggcgttgcc tttctggtca aggccggcat gtggccgctc tgcttctggc
     4381 tcccctccgc ctacacggcc gccgcagcac cggtgggcgc actctttgtc gcgctcacca
     4441 aggtcggggt ctatgtgctc ctgcgcctga ccctgctgct cttcggcagc gacgccggag
     4501 aacttaccgg cttcggcggc ccggccctgt tgtggggcgg aatagcaacg ctcgcattcg
     4561 ccacatccgg cgcgctcgcc tcccaggcca tgggtaggct tgccggatat agcgtgctca
     4621 tctcttcggg aacgctgctt gcggccgtcg gcatgggcga cgccaccgtg gtttcgggcg
     4681 cgctgttcta cctcgtcagt tccactttca gcacggcggc cttcttcatg ctgatcgaac
     4741 tcgtggagcg cgcgcgcgaa cccggagcgg acctgctcgc cgtaacggtg gaagcatatg
     4801 gcgaggaaga agacgaggcc gaggaggagc gcgaggacga agaggtcggc gttgttctgc
     4861 cgggggcgct cgcagtcctc ggcgtctgct tcggatgcgt cgctctcctg cttgccgggc
     4921 tgccgccgct cagcggcttc atcgccaagt tggccatgct gacgggcatg ttgaacctcg
     4981 aggagctggg caggccggca gacatcccgg cgcgcacatg ggtgctggtc acgctcatca
     5041 tcctgtccgg cctggcagta cttgtggcca tgatgcgcag cggcatccgg atcttctggg
     5101 cgccgctcga gacgatcatc ccgcgggtgc tcgtggtgga ggtcgccccc gtcgtggcgc
     5161 tgctggtgct ttgtctgatc ctgaccgtcc agggaggcgc gatgatcagc tacaccgagg
     5221 ctgcggcccg atcgctgcac gacccgtcag gctatatcca gggcgtgctg ggaacattgg
     5281 aaacggcgag gggcgaaaga tgagccgcgt tctgccctat cccgtattga ccgcagcact
     5341 cctgctcatg tggatgctgc tgacgtcctt ctcgccaggg cagttcctcc tcggtgcggt
     5401 catcgccgcc ggcgcttcca aggcaatggc ggcgctgcac ccgtcgaagc caagggtgag
     5461 acgctggcag gccatccccc gccttgccgg gatcgtgatg ctggatatcc tgcgctcgaa
     5521 tgcggcggtc gcgagcatca tcctccaggg ccggcaccgt gaccgggtgg caggcttcgt
     5581 ggccatacct ctcgaactgc gcgacaggac gggactggct gtgcttgcgt gcattatcac
     5641 cagcacgccg ggcaccgcat ggatcgaata ttcggtcgag accggcgtcc ttatcatcca
     5701 cgttctcgac ctcgtggacg aagaggaatg ggtcgccctg ataaagaacc gctacgagaa
     5761 gctgctcatg gagatcttcg aatgaccgca gacattctcc tttggtccgt gacggttgct
     5821 caggcaatgc tcgtcattgc gatggggtgc aacacgtaca ggctgatccg cggaccgcgc
     5881 gcgcaggacc gggtgctctc cttcgacgcc atgtacgtca tcgccatgct tctcgtgctc
     5941 accttcggca tccgcacggg aaacgcgttc tatttcgagg cggccctgat cattgccgcc
     6001 ctgggtttcg tctcgagcgt ggctctcgcg aagttcctca tgagaggcga ggtgatcgaa
     6061 tgacgcatgc tgccgacctg cccgcctggg ccgcgcttct gacagctctc ctcgtccttc
     6121 tcggcgccgg gctcacgctc gtcggagcga tcggaaccct gcggctcggc agcttcttcg
     6181 agcgggtgca tgcgccgacc ctcggaacaa gttggggaac gggcgccatc gtgctcgcct
     6241 cgatcgtctg cttctcggtg ctgggctcgc gggtcgtcct gcatgagttc ctgatcggcg
     6301 tcttcatcac cgtcaccaca ccggtcacgc tcacgctgct tgtccgcgcg gctctctatc
     6361 gcgagcgggc ggaggggaac acggacgtgc ctgcgcgttc ggtccttcgc gcgccccaaa
     6421 gcctcaaccg gcgggaaccg ggcggcgaca ccgctcggta atcgcggcta cgctaagccg
     6481 atgagtacgc gtcgattgtt tccaagcgtc gaaaggccgc atcgcatctg atccggttga
     6541 cgaaggaagc tggaatggcc atatcctggc catgaatact ccacgtccat ataagaaata
     6601 cttggtcgtt tcggcgtcgc tcctgacgct cccgtagccc cgagcggctc gaacgtttcc
     6661 gttcgccgtc tcggggacct cccactctca ttcaatccaa gccggctggt acggcatctc
     6721 ttccgcagcg ctccgacgag catccgcgga gcgaaggttg atgccaccag caagccgtcc
     6781 gcggatggcg catttctgcg gccaggcggg gaaggctggc gaccgtgaaa ggaggtacga
     6841 tatgtccgca tacgaaattc gatcaagcac cgcccgaaac aatcctgcgc actacacgcc
     6901 gcgggtgacc atcgcgcagc ttttcctgcg tgcggtccaa tacgcggtcc agcgctggca
     6961 gcgcaggcgc gcgatcgccg aactcgagag cctgagcgac cgggagctca aggacatcgg
     7021 aatcgcgcgg cacgacatcc cgcgggcggt cgacgggctc atccggtcga gagacggctc
     7081 ctccgagggc cctcgccgcc gcgagcccgc cgggcccccg agcgggtccg aaaggcagcc
     7141 tgaagaacaa cgaaaaggat cgaaccttgg caggtcaggc gtcctgcgcc tggcttacgg
     7201 aggcaacgat ggacacgaca acgagaatga tcatcgcagt gggcatgttc gggctgatgt
     7261 tcgtggcttt cgcaatgatg tgaccacgcg gtccggcgtg gcgcgcgaca cggtatcggc
     7321 ctgagggagg cctttcggct aggtgaccgt catgtgcatc gagactttcg tcaggatttc
     7381 gaggcttacc gggaaaggac gggtccattc ccggagcagg ctttctcacc ctgaatcccg
     7441 cacctgaacc tgctccgggg catagcagaa acgcggttca ttcccgcggc gcttcacgac
     7501 gccgcgctga tggagcgtga catgagaacc ttccgtccga agcctgtctg cttttcctgc
     7561 agccgcatcc tgccgagagc ggccgcccgt tgcccgtggt gcttttctcc ggtcgccgcg
     7621 cctgtgacat cggcggatcg gttcaagccg actcgccatc cggccgcccg cccggcatgg
     7681 tcacaggtgc tgctgtgaac ggcgatcgca gggcgcatgc cttctcagac aaggctcatc
     7741 gtccggatgc ggcgccggtc gtggctccgc atccggcatc ggcgcgcgag gcggctttcc
     7801 aggcggaacc gaagacgaag ctgagcgaac ttcttggagc gcaggacatt ctgttcgaga
     7861 cgcgatccgg ccggaagtgc acctccctgg gggccatagc cgtccggctg ggggaaaagg
     7921 tcggcagaag ccaaggctcc gtgctggccg ccctccttcg tcgcgagcgt ctcgggtcga
     7981 ccgcaatcgg caatggcacg gcaatgcccc acgcgcgcct cgacggaatc cgtgctccgg
     8041 cggctgttct ggcgagattg cagcggcccc tccaattcgg aacgtcggac gacgaccgcg
     8101 tcgacctgct gctgggcctc gtatggccga aatcagaccc gcgaggcttt gtggccggcc
     8161 tgcatcatgg ctggcgtctc atgcggcgga gtgggctggc tgatctgttc cggcaatcgc
     8221 ggacgcccgc ggaggcgctc gccttgatca aatctttcga ggaagagaag tcggatgcgc
     8281 gaggccacat cggtccggca gcccccgcca tgaccggacc gggcggctcg tagccacatg
     8341 gttctggcta tgaagcaccg gatcaacgag cttccggtga cggtgcccgg aacggcgagg
     8401 gttccgggat gtcatcgggc gtagaaatgt tttaggggtt cgatacgtgc aactttcgga
     8461 acatcatgac gatctcgaac gacaacgagt ttggcaggct gcgagaaatt ggccggattg
     8521 ttgccaatgt tctggaggcg atgggagcag ccatcgaacc cggaatgacg acggccgagc
     8581 tcgatgagct tggccgccgg ctactgaagc aggaaggcgc ccggccggct cctgaattgt
     8641 gctacggttt cccgggagca acctgcatca gcgtgaacga agagatcgcg cacggcatac
     8701 ccggcgggcg tcgaatagcc gcgggcgacc tcgtcaacat cgacgtttcg gcggaaaagg
     8761 acggggtttt tgccgatacc ggcgcgtcct tcaccgtgcc tcccgtgtcc agggccatcg
     8821 agcggctctg cagggacggc cgccgcgcca catggacggg aataaggcag atcgcccctg
     8881 acaggccgtt ggcgaatatc ggttttgcga tcggcgcgtt cgcgcacaag aaccgctata
     8941 cgctggtcca gaacctggca agtcatggtg tcggccgctc gttgcatgaa gagccgagcg
     9001 aagttgcgac ctggcctgac ccgacggaac gcaggaaaat gcacgaaggg ctggtgttta
     9061 cggtggagcc gtttctctcc atgggagcga aatgggcgac cggcggcgac gatccgtgga
     9121 cgctgttcag tgaacctcac gccccgaccg tgcagtacga gcacaccgtc gtcgtgggcc
     9181 gaaacggtcc ggttgtggtc acactgctgg gctgagcctc gttcatccgt gtccgcgtct
     9241 ccccggtacg gaagaccggg cggttgcgcg gcaatcaacg acagggcgcc ggttgtcgca
     9301 cagcgtcgtt cagatcatga atggcccgtt gatcggccag tttggggtgt caactacgct
     9361 gcgaggctgg cgatctcgca ccgggcccct ttgatggcgc tggccttggc ctcctcgccg
     9421 aatgcaacgc cttctgcgcg tacggtggtt atgtccgtaa tcccgaagaa gccgaagatc
     9481 gtgcgcaggt aggtttcctg atgatcgagc gcctctgacg ggaacccgcc gcgggaggag
     9541 gcgatgatga ccttcttgcc tttggcgaga ccctccggcc catcgtcggt atagcggaag
     9601 gtgacgccgc gcaccgcgag gcgatcaatc caggccttca actgacttgg aatggcaaag
     9661 ttgtacatcg gtgctccaat cacgacgatg tcggcggcaa gaaattcctc cagcgctgct
     9721 tgtcctgcgg caagatcgtc ctggatcgcc gcctcgccgg gaacagcccc ctggtctgcc
     9781 agaagatggg cgtccgacag atggccgatc ggttgggccg cgagatcgcg gaccgtcact
     9841 tccgcttccg gctgcagccg ccgcagggat gcaacgattt cggcggagag cgcccggctg
     9901 accgaggcgc ggcctgtgat gctggtgtcg acgtggagta ttttcatgga gaaattccct
     9961 tgggcgagtt gacatgtggc ttgaacttag gcccgtcact cgccagaaaa aaccgggata
    10021 gatgtgggga ttcctatagg aaaagttgat ggagtttgga gtcagcctcg accagttgcg
    10081 cgtatttgcg accgtagtag aggaggggag cttctcggcc gcagccaggc ggctgaaccg
    10141 ctcacaggcg tccattacat acgcggtcca gaagctcgaa agcgccgtcg gcaccctgtt
    10201 gttcgaccgg agcggatacc gccccacact gacggatgcc gggcgtacac tccttccacg
    10261 cgcgcgccgg gtggtcgaga ccagtgcagg cttcgagcgt caggcacgag cgctgcgcga
    10321 gggcatcgag gcggaactga ctttggtcgt cgatgcgatg ttccccatgc cgcttctttt
    10381 cgctgcctta tcggcattcg atcggcagtt tccgactgta cagacgcggc tttatgtcga
    10441 gtccttgggg gcggccgtca aaacagtgct ggacgggaat gcagaccttg gcatcgtcat
    10501 cgacttcgcc aatgtgtccg atagactggc ctcggctgcc atgaacgaga tcgaactcgt
    10561 tccggttgcc gcgcccacgc atcccctggc gcttatgcaa gcgcaaagcg acaaggccct
    10621 aagcgacgag gagctgcgcg atcacctcca gctcgtgctc acagatcggt cggaactgac
    10681 caaagggaag gataccggcg tcgtcagcgt gcgtacctgg cgtctggccg atctcggcgc
    10741 caagcatgcg atgctgctgg ctggaatggg ctggggcaac atgccgctgc acatgatctc
    10801 cagtgacctt gccgcaggcc gtctcgtcga actggagatt gccaggtggc cgaaaacccg
    10861 gcggtcgaca gtcgccacgg tcatcgtgca tcgcctcgac cggccgccgg gcccggcagg
    10921 cgcatggctg cttagccggc tcgcgggaga cgaaaggtga tcttctgctt cgatccgctt
    10981 ccatcggtcc cgggtggccg tcgcgttctg acgctgcgcc cgcgctacag attgccgcct
    11041 gcggcttgtg ttgggtgggc tttgccgggc ttgatcgcga cgtgacctta gaagtcaata
    11101 tcgcgactgt cgtcgaaagg gtcgccggcc gccgacctgt tcgacccggg cttgggccag
    11161 acggtgtatt ctgccagtga aatggagaac gtgtccggcg ggcgaggttg attgcacggt
    11221 cgaatgcgca gggcatttca acgacaacgt cctccgttcc gcagcggtgg tggtcatccg
    11281 aggctctccg gctgcggcct tctctcatcg atccgtttga tcgagaagct gaaaggagac
    11341 gtattgcttg aaatctgccg aagtgatcga cgaggaacaa cgtccggcgg ggcgggccaa
    11401 gtcgctttca ctgtgctaat gacgccgacg ccgcgactct attttagata tcccagccac
    11461 tgcggaagcc aaagcgagat agacgggatg agcatgatga tcgcgagaaa cacgacttgc
    11521 accacgtaga acggaatcag cgccatgctg atttcttcga tcttgagcct gctgatgctg
    11581 gcagcggcaa aaagacatgg gcccacgggc ggagtgttga gtcccaccac aagcgagaga
    11641 gacatcacga tcccaaaatg taccggatca atacccgcgc tgatcgcggt cggagccagt
    11701 atcggaccca tgacgataag ggccgcacca acatccatga acattccgac gattagcagc
    11761 aagccgacga tcagggtcag cagcaaaatc ggatcagcgg taatgccgac gagcgccttg
    11821 gccatcgttt ccggtacccg ctcgaggcct ataatccaac caaaaatgct tgccgtcgct
    11881 aggaccagga aaatgacaga cgtcatgatc cccgacctca gcagacattg atatatgtca
    11941 gccagcttca gagttcgttt tgcaaaaagt cctatcacca aggcatagaa tgctgcgacg
    12001 gcggccgctt ctgtggcggt aaaaatgccg ctgaggatac cgcccatgat gatcagcggc
    12061 atcaacaaag cagaagatgc ccggactgtg atgctcaaaa actcgacggg actataacgc
    12121 gtgttccgtt tcggaaagtc atacttcctc gccatgaacg cgacgacgat catcaacatg
    12181 actgtcagca atattccggg cagcacggca gccagaaaca tggtggccac cgatacctgc
    12241 atgatcgatg catagaggag gatgatgatg ctcggcggta ttatcggccc gatgagcgac
    12301 gaggcaactg tcagggcagt cgcaaagcgc ctatcgtagc ctgcagcttc catcgcccga
    12361 atctcgatgg cacccaaacc ggcgacatcc gcaattgccg aaccggatat cccggcaaag
    12421 aaagcgctcc ccaagacatt cacatgagca agaccgccgc ggaatctccc gacgatggca
    12481 tctgcaaact cgacgaggtc atcgaggatc tttgaggcgg tcatcagctc ggcagcgaga
    12541 aagaagaacg gaatcgccat gatcgtaaag acattgagcc cggcgaacat gcgctgagga
    12601 accaggagga gaaaatccgc cccgccgacg gcaaacatat agcccaccga catgattccg
    12661 accgcgaacg cgatgggcac tccgataaga agcaagagtg caagggcgat catagcgagt
    12721 gtcatcaact ttcctcagct cgcctcgcga ctaagcgacc gtttctccag cctcgaatgg
    12781 cgagttcccc gagcgggcgc gtttgattcc ttcggaaagt gatgctccca gctggacgag
    12841 catcatgaat gcgcccaggg gcatggagaa aaagacgaca gtcatcggta ttccaagcgt
    12901 aggcgcgcgt tggagcggtc cagcttcgaa cgtcaagcgg aagctgtaga ccacaagcag
    12961 ggccaagaac aatgcggtca acaccagccc gacaatatag gccgcaaaac gtaatctcgg
    13021 aggaaccgag tcaaccagga acgtcactcc gacatggtcg agttgccgca ccgccacgct
    13081 gcccgccagc atggtgattg cgaccatcat gaaacgcgca gcttccggtg gccaactgag
    13141 tgggtctgaa acaacaaacc tgaaaaacac gccgagcagg atggagatgg tcattcccgc
    13201 catgagaatg accgtaagta ttttcgcgat cgccagcaaa aggtcggcaa cacgcatgaa
    13261 tgtcttcatg tctcaattcc cggaaagggg cgcccggcct gtcccgagcg cggggagggg
    13321 ggatgattag ttggtcatcc ccaactcttc ttctgcgctt tcaattgcgt cgaagaactc
    13381 gtccacatat gcctttccaa actgttcaac catccagtca acgactggcg gttgcgctgt
    13441 tttgcggaat gcctcctgct cctcggggct tgcgacggtc acgacaacgt cctgtttgcg
    13501 gaactcgtcg atcacgtcgt tcaccagtga ttgcgacaaa ccgcgtcctg cgacctgtgc
    13561 ctgttttcct gcgatgatga ccgcctccct aagatcgtca ggaagggact gataccattt
    13621 ctcgcttatg acaatgagcg ccagactgta gacatgctgg tcgatggtca catatttcag
    13681 gacttcttgc agattctgct gcagcgttcc gataatagga agctctgccc cgtcgaccac
    13741 tcccgtttgc agagctccat agacctcgag ccatggaata gcagtgggag aggctcccaa
    13801 gctcgtgacc atcttctggt gtgcgggaat ttccatggtg cg
//
LOCUS       DASVDU010000018        32323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1704053, whole genome shotgun sequence.
ACCESSION   DASVDU010000018 DASVDU010000000
VERSION     DASVDU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 32323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32323
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704053"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /gene="tsaB"
                     /locus_tag="VF226_01275"
     CDS             <1..663
                     /gene="tsaB"
                     /locus_tag="VF226_01275"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX7072649.1"
                     /translation="CFGACSAAILWNGQLTWRYEEMATGQAERLIPMIGEVLDQSGAS
                     LRDLDTIAVTEGPGTFTGTRVSVAAARGLALATGLPIRATTSLHVMAYRAISELGEAA
                     AGSVLAVCVDARGGKVFVQVFELQATEPLTQATVMAPDAAAALEAPAPLLCVGSGGLS
                     VSEAARRAGREAEAKLPTLQPDARYLARLAASLPVRDPLVPLYLRPPDAKPQTGKSLP
                     RA"
     gene            660..1145
                     /gene="rimI"
                     /locus_tag="VF226_01280"
     CDS             660..1145
                     /gene="rimI"
                     /locus_tag="VF226_01280"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7072650.1"
                     /translation="MMTSERPAILELIEASSPEVNRILSALHAALFTPAWSSDDFASL
                     HSHPGSIALLARSISGRRPIGFILGRVVVDEAEILSVGVLPEAQRQGIGSRLVGEFAR
                     LAAERGATRLFLEVAADNSVARQLYRAQGFCIVGRRPGYYEHPAKAPTDAIIMSKLIG
                     G"
     gene            1211..1678
                     /locus_tag="VF226_01285"
     CDS             1211..1678
                     /locus_tag="VF226_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7072651.1"
                     /translation="MEGHWLNRMAGAEDQSPSRIERLCSERGLRMTGQRRTIARVLTE
                     AEDHPDVVEVYRRAHQIDPRISLSTVYRTVKLFEENGILERHEFAAGRRRYEEATREH
                     HDHLINLETGQVIEFRNEDIEKLQERVARELGFELVGHRLELHGIPLRRKKST"
     gene            1717..2547
                     /locus_tag="VF226_01290"
     CDS             1717..2547
                     /locus_tag="VF226_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7072652.1"
                     /translation="MRTDPTPSSNRRGAPRAAAILAGFAGLTLPLMPVQAGLLRISPH
                     YARKFPHWYHRQVCRLLGIRLHVEGKIAENTPVLVVANHVSWLDIPVLSAVAPVSFVA
                     KKEVGSWPFVSSLARLQRSVFVDRERRTAVGQTTTEIIERLKSGDAIVLFAEGTSSDG
                     NRVLPFKSPLFAAVKPTLANGLAAAPGACVQTLAIAYTHLHGIPLGRSGRSLVGWYGD
                     MEMAAHAWELLKAGPLDVKISIDPPVSLSDFADRKELARYTEDRIRQALARLLRGRND
                     "
     gene            2754..4091
                     /gene="miaB"
                     /locus_tag="VF226_01295"
     CDS             2754..4091
                     /gene="miaB"
                     /locus_tag="VF226_01295"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7072653.1"
                     /translation="MPGARKVFIKTYGCQMNVYDSERMAEALAQHGYSETEDPAQADM
                     IVLNTCHIREKAAEKVYSELGRMRQLKVAREVDGKKTLIAVAGCVAQAEGAEIIARAP
                     IVDLVVGPQAYHRLPELAAKAQAEHRQLIDTEFPDEDKFAHLPERRGTRPATAFLTVQ
                     EGCDKFCTFCVVPYTRGMEISRPVVQITEEARRLVEQGVREITLLGQNVNAYHGEGPD
                     GRTWSLAKLLRHLSSIEQLERLRYTTSHPHDMDDELIEAHRDLPKLMPYLHLPFQAGS
                     DRILAAMNRKHTARDYLRIVEKIRAARPDIALSTDIIVGFPGETEQEFEDTLKLVQQV
                     GFAQAFSFKYSPRPGTPAANIEEQVPEEVKAARLARLQELLQAQQRQFNARCVGRVFP
                     VLFEKLGRKAGQLVGRSPYLQAVHAEAEPSLLGRILPVEISDASTNSLAGVVR"
     gene            4207..5247
                     /locus_tag="VF226_01300"
     CDS             4207..5247
                     /locus_tag="VF226_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7072654.1"
                     /translation="MNAYRGAAASGAGRPTRASSARKRLVVPFESNRHLAELLGEYDA
                     HLALIEDRLGIEAHAHGNVVILSGSEPACNIARNVLEQLYARVARGEQVGPGDFDGLI
                     RHGREEKPGKSPDGQAQITTRRRVIKARTPTQSQYIRGLESTDLVFGLGPAGTGKTYL
                     AVAYAAYCLERGIVERIILSRPAVEAGERLGFLPGDMREKVDPYLRPLYDALYDVLPP
                     EKVGRDLETGVIEIAPLAFMRGRTLSNAYIILDEAQNTTSMQMKMFLTRLGENSKMVI
                     TGDPSQIDLPVGQRSGLEEAVRLLAGIQGIEVVRFTSSDVVRRDLVARIVEAYDRGQA
                     AEAKRERHERDT"
     gene            5231..5779
                     /gene="ybeY"
                     /locus_tag="VF226_01305"
     CDS             5231..5779
                     /gene="ybeY"
                     /locus_tag="VF226_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX7072655.1"
                     /translation="MNETLETVIAEPPSDGGDDDPEPPGRLQLDIIIEDGNWGALAPV
                     DEVFGPVADAVARRLQLDSAEAAIALSSDERVRALNLTYRGKDKPTNVLSFPAGEDGL
                     PPGSRRHLGDIVLAAETVAREAAAEGKPLHHHLQHLAVHGLLHLLGYDHETEVEAAEM
                     EALEVEILGQLGVPDPYAPESL"
     gene            5807..6871
                     /locus_tag="VF226_01310"
     CDS             5807..6871
                     /locus_tag="VF226_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7072656.1"
                     /translation="MSIDVDPSRSEQKSDDGSEGQSWLSSLKGLLRLSGTPTFRDALE
                     NALKTGTKTEDAFSAEERRMMLRLLHFGRLRVDDVMVPRADIIAIDESETLGELFATF
                     EEAGVSRIPLYHETLDDLRGMVHIKDLMSWLYSEAAVLSEESDGEEAEAQGGTRRSNG
                     HNGQAKPRLRALDFSRVDLTRPLAVAKIRRPVLFVPPSMPAMNLLIRMQTTRIHMAFV
                     IDEYGGTDGLVTIEDLVEQIVGDIEDEHDEAEEANIKFDPKSGLVASARTPVQELEEY
                     LGIKLLNNDEEEEIDTLGGLVFALVGRVPARGELVQHPSGVEFEVLDADARRVNKLKI
                     HTDRAPKAGDGGSAAGQLRS"
     gene            6872..8578
                     /gene="lnt"
                     /locus_tag="VF226_01315"
     CDS             6872..8578
                     /gene="lnt"
                     /locus_tag="VF226_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7072657.1"
                     /translation="MSRVGGSGPAPALISSLDGLHRAACFIRKLTGWWRICVAFISGG
                     LCALAMAPFFLWPVLFLSFPVLTWLIDGALDPGKQPAESEIRSATAHARNAAVVGWWF
                     GAGYFLAGLFWIGEAFLVEAERFAWLMPFAVMLMPAGLALFYAAATAAAALLWRPGLV
                     RVLALALTLSAAEWLRGHILTGFPWNVLGYALTWPLPLMQSAGLFGIYGLTLLAVLIF
                     SVPAVALADAAAGHGRVRTGLAAALLAMLPLPLAWAYGSYQLAEPRPAPIEGVRLRLV
                     QPSVPQRNKWRPEHQRDIFIDHLNLSQRAPDGSFDGLAGITHVIWPEAAMPFLPLDTP
                     EALAAIGRMLPEGTQLLAGALRADGAVSPAQGRRAFNSLIAIDSDGRPTAIYDKVHLV
                     PFGEYLPLKPLLERLGLQKLVGWRGGFSVGRSPRPLFEVAGLPPVSALICYEAIFPGA
                     VVEPGQRPRALINITNDGWFGETTGPHQHFHQARVRAVEEGLALIRVANNGITGVIDA
                     SGRVLASLGLNERGVIDTDLPGPKPPPLYARFGDAVFAALWLAGFCWLAGSLWRKKST
                     AH"
     gene            8710..9195
                     /locus_tag="VF226_01320"
     CDS             8710..9195
                     /locus_tag="VF226_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7072658.1"
                     /translation="MARQDDAVAEHDFDVVEKGSRRANPLDLHVGSRVRLQRMLLGMS
                     QEKLGEQLGLTFQQIQKYEKGINRIGASRLYELARVLGVSVQFFYEDAPGTTSEYRSQ
                     PNSAAGGLAERAADRYVVDFLSSREGVELNKAFVKISDPKVRRSIVDLVRTLAGEEKD
                     S"
     gene            9317..10516
                     /gene="metK"
                     /locus_tag="VF226_01325"
     CDS             9317..10516
                     /gene="metK"
                     /locus_tag="VF226_01325"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX7072659.1"
                     /translation="MSRQSYLFTSESVSEGHPDKVCDRISDEIVDLFFREAPKAGVDP
                     RDVRVAAETLATTNRVVIAGETRGPATITKDLVAHLARMAIRDIGYEQEGFHWEKAAI
                     EVLLHPQSADIAQGVDADKEKDEGAGDQGIMFGYACRETPELMPAPIYYSHKILEVLA
                     DARKRKEGDAALLGPDSKSQVTVRYEGGKAVGVTQIVLSTQHLAPELTSAQVRDIVEP
                     YIRKILPEGWIDANTAWHVNPTGKFVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFS
                     GKDPTKVDRSAAYAARYLAKNVVAAGLADRCTIQLAYAIGVAEPLSIYFDTYGTGQVP
                     EEQLEKAVRKVMKLTPRGIREHLGLNKPIYARTAAYGHFGRKPDADGGFSWEKVDLVD
                     ALKSAVG"
     gene            10600..11292
                     /gene="trmB"
                     /locus_tag="VF226_01330"
     CDS             10600..11292
                     /gene="trmB"
                     /locus_tag="VF226_01330"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX7072660.1"
                     /translation="MQRRPREDIRSYGRRHGRKFSARQERLLAEQLPRVAIDLASPPP
                     AHIVDLFACPVRDVWLEIGFGGGEHLVWQAEHNPDVGLIGCEPYLDGVVKVLAAMEEK
                     GLRNIRILADDARPLLRWLPQASIGRVFILFPDPWPKTRHRKRRLVSASFLETLAKVM
                     KPGAELRIATDIGDYARTTLLAFRATPAFEWLAEAPSDWRERTFDWPPTRYEQKALAA
                     GRRPTYLRFLRT"
     gene            11504..12097
                     /gene="rimP"
                     /locus_tag="VF226_01335"
     CDS             11504..12097
                     /gene="rimP"
                     /locus_tag="VF226_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7072661.1"
                     /translation="MDQRDEGQSARRFIRETGLAAQIAELVEPTLEDLGFRLVRVQIH
                     GRDGQTVQIMAERPNGTMTIEDCEAISRQLSPLLDAHDPLPGSYRLEISSPGIDRPLV
                     RPSDFEDWSGYEAKIELKQQVGGRKRFRGVLEGFEDGEVRLECTLDQQGTQVLGFPVD
                     LVADAKLVLTDELIREALRRAKKSSKGDGGSGPPQED"
     gene            12116..13762
                     /gene="nusA"
                     /locus_tag="VF226_01340"
     CDS             12116..13762
                     /gene="nusA"
                     /locus_tag="VF226_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7072662.1"
                     /translation="MAVAVSANRLELLQIADAVAREKSIDRKIVIQAMEDAIQKAAKS
                     RYGADNDIRCEIDPRTGDTRLSRVLAVVEKVENDATEISLEEAKRRNPEAKIGDLIAE
                     PLPPLDFGRVAAQNAKQVIVQKVREAERERQYNEYKDRVGEIVNGTVKRVEYGNVIVD
                     LGRAEGIIRRDEMIPRENMRYGDRVRAYIYDVRREQRGPQIFLSRARPEFMSKLFAME
                     VPEIYDGVVEIKSVARDPGSRAKIAVISKDSSIDPVGACVGMRGQRVQAVVNELQGEK
                     VDIIQWRPDAAEFIVNALAPAEVTKVVLDEDSNRIEVVVPESQLSLAIGRRGQNVRLA
                     SQLTGWDIDILTEQEESERRQKEFAERSQIFMEALDVDEVIAQLLVTEGFATVEEVAY
                     VDLSEIAHIEGFDEDTAEEIQTRAREYLERIEQERDARRKELGVSDDLAQVPGMTTAM
                     MVALGENGIKTLEDFADCATDDLVGWTERKKERDAEPVRHKGILDGFDVGRKEAEDMI
                     MAARIILGWINPEDLEQPAEEAVEGAEAVEETTGEAGSSA"
     gene            13796..14473
                     /locus_tag="VF226_01345"
     CDS             13796..14473
                     /locus_tag="VF226_01345"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX7072663.1"
                     /translation="MAVRLDSAERPAAERTAPLRTCVVTRDQKAPEELIRFVPSPDGE
                     IVPDVARRLPGRGVWVTAERGYVAEAVKRKAFAKSLKRQVSVPPDLPDVVERLLVKRA
                     MDALSLANKAGLVITGFSRIEAAIAAGTVAALLHGQEAAADGAGKLDRRFRAVCKQIG
                     KPAPIIQELTVDQMSLALGRSNVVHAALSAGGATTNFLNEVGRVTRYRSGASASNTRY
                     AADQGAV"
     gene            14496..17225
                     /gene="infB"
                     /locus_tag="VF226_01350"
     CDS             14496..17225
                     /gene="infB"
                     /locus_tag="VF226_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7072664.1"
                     /translation="MSETKETTEKTVLTGTRKPLSLKRTIESGHVQQKFSHGRSKSVV
                     VEKKRKRTIAGQEPEETAAAPVQVRPRPHVAQQRPAPKAEQQQQPKILRTLSEEEQQA
                     RARALAAAQVREAEEQRLREERMRQEAEQRRREEAERKAAQLTEAAAKEAEPERAEPA
                     AEAETAAQPEAPAMAETPAAPEEPAAPAAAAEAAAAPEEPETPEVPQVLPQPIGMTRP
                     IIVSRPERSQPKAAETAKKAEVEVPKRGAKAAALAEIEEEEGRTKKKGGKLVRAPLKG
                     ADESRVRGKLTITNAFDERQRERSLASLKRRRERERLRAMGIPQQREKIVREVVIPEA
                     ITIQELSNRMSERAVDVIKFLMKQGAMHKITDVIDADTAELIVQEFGHTPKRVSEADV
                     EEGFIGAEDAPETLRPRPPVVTIMGHVDHGKTSLLDALRQTNVAAGEAGGITQHIGAY
                     QVTTPTGEVVTFIDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTVEAINHAKA
                     AGVPIIVAINKIDKPDADPTRVRTELLQHEIVVESMSGEVLEVEVSALKRINLDKLLE
                     AISLQAELLELKANPNRSAEGFVIEAKLERGRGPVGTLLVQRGTLRVGDIVVAGTSWG
                     RVRALINDRGENVKEAGPSVPVEVLGFDSAPEAGDQFAVVESEARAREITDYRIRKRR
                     DTLGSVGTARTLEQMMQQLKDAEKKEFTLLVKGDVQGSVEAIAGALKKISTDEVEARI
                     VHAGVGGITESDVALAHTSGAVIIGFNVRANAQARAAAERDGVEIRYYNIIYDLVDDV
                     KAAMSGLLAPTLREVFLGNAEVLEVFNITKVGKVAGCRVTEGKVERGAKVRLIRDNVV
                     IHEGTLSSLKRFKDEVKEVPAGQECGMAFANYQDIRVGDVIECFHVETIKRSL"
     gene            17341..17721
                     /gene="rbfA"
                     /locus_tag="VF226_01355"
     CDS             17341..17721
                     /gene="rbfA"
                     /locus_tag="VF226_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7072665.1"
                     /translation="MPRNAPARSVPSQRQLRVGELIRHALSELMTRGEIHDDVIAEHV
                     ITISEVRMSPDLRLATIYVLPLGGVDTEPVLAALERNKRFIRGVVARAVNLKFAPDIR
                     FRKDESFERGERIDRLLDSLKERE"
     gene            17788..18711
                     /gene="truB"
                     /locus_tag="VF226_01360"
     CDS             17788..18711
                     /gene="truB"
                     /locus_tag="VF226_01360"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7072666.1"
                     /translation="MARAKKGNPIHGWLILDKPQGMTSTQAVAAVKSLYNAQKAGHAG
                     TLDPLATGILPIALGEATKTCSFAVDGEKAYRFTVRWGTETSTDDAEGEVIQTSDRRP
                     TAEDIEEILPRFTGEISQVPPTYSAIKINGERAYDLAREGEKPVLEPRPVLIEELRIV
                     ERLDESTTVFEAVCGKGTYVRALARDMGREMGCYGHVIALRRTQVGPFGEEVAVPLDE
                     LQDASRLDGAIEALKAKLLPVESVLDDLQRISLSSADASRVRSGQNVLIRGRDAPILR
                     GPAYAMAKGQLVAIGELAKGEFRPTRVFNLG"
     gene            18897..19166
                     /gene="rpsO"
                     /locus_tag="VF226_01365"
     CDS             18897..19166
                     /gene="rpsO"
                     /locus_tag="VF226_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019295116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7072667.1"
                     /translation="MSITAERKQELIKENATKSGDTGSPEVQIAILTERINQLTEHFK
                     IHKKDNHSRRGLLKMVGQRRQLLDYLKRKDQARYQRIIEKLGIRR"
     gene            19399..21594
                     /gene="pnp"
                     /locus_tag="VF226_01370"
     CDS             19399..21594
                     /gene="pnp"
                     /locus_tag="VF226_01370"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7072668.1"
                     /translation="MFDIHRVEIEWGGRKLKLETGQLARQADGAVLAQYGETTVLATV
                     VAAKTARPGVDFFPLTVNYQEKTYAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FVEGFKNETQVIATVLSFDNENDPDIVAMIASSAALTLSGVPFLGPIGAARVGLIDGE
                     LVLNPLIDEMEKTKLDLVVAGTQDAVMMVESEAHELSEKQMLDAVMFGHRHFQPVIQA
                     IIKLAETAAKEPWDIAIPDKAKYKDRVKEIAEEELRRAYSMTEKQKRRETVEAIRQKV
                     FEELLPADDPTDAVLLGDVFKSLEQDIVRGNILQTGRRIDGRDLKTVRPIMAQVGVLP
                     RTHGSALFTRGETQALVVATLGTGEDEQYVDTLEGTKKERFMLHYNFPPFSVGETGRI
                     GSPGRREIGHGKLAWRALNPLVPSADEFPYTIRVVSEITESNGSSSMATVCGSSLAMM
                     DAGIPLKRPVAGIAMGLIKEDDSFAVLSDILGDEDHLGDMDFKVAGTAEGVTSLQMDI
                     KITGITEEIMRVALDQAREGRLHILGEMAKALGQARAELGEHAPRIETIHIPTDKIRE
                     VIGSGGSVIRSIVEESGAKIDIEDDGTVKIAAARRESIDAALKRIREITSEPEVGEIY
                     KGKVVKIMDFGAFVNFFGSRDGLVHISQLTSKGRPSSPSEVVKEGQEVYVKLLGFDDR
                     GKVRLSMKVVDQETGKEIPPEPRPEGEEAAEPRGERRERRERRRDREAS"
     gene            complement(21744..22256)
                     /gene="bamE"
                     /locus_tag="VF226_01375"
     CDS             complement(21744..22256)
                     /gene="bamE"
                     /locus_tag="VF226_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEX7072669.1"
                     /translation="MTKRKHGQPVGASPVRLREAARRGVLLAGAASLAAILAACSSAA
                     IVTKHGHSFQPSELQQIQPGMTQEQVRMVLGTPTTTSSVGTGDAFYYISSTTKQVAFL
                     APEEVDRRVVAIYFTRAGTVERIAEYGLKDGKVFDFISRTTPPANSYEDGLFKQIFRN
                     LGKRTNIFGS"
     gene            22469..22927
                     /locus_tag="VF226_01380"
     CDS             22469..22927
                     /locus_tag="VF226_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEX7072670.1"
                     /translation="MAAARREPFYSVYGVPDTPDGRFAMIIVHSYLVLERLQHTGGPG
                     QTLTRPLVEAFVTDMDDCMREMGVGDLTVPRKVKKAAGALYECAAEFRKAEQAGSEAA
                     LTEAVHKKLLPFAPRSAAASVAAYMRRATATLAACPDADLLSGRATFEDV"
     gene            22941..23471
                     /locus_tag="VF226_01385"
     CDS             22941..23471
                     /locus_tag="VF226_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7072671.1"
                     /translation="MSAAMTSELEWSHRVSDIPETGLAAERHATPEARAKVAAELDLL
                     DCARLDARYEVKPLKHGRYLLQGELEADVTQTCVVTLEPVEGHIVEPFEVEFRPGPPA
                     EQVVEFNALEVRDIEPLENGTIPVGRIVYEHLASALNPYPRKEGVAFEQPASEQGEEE
                     ASGSPFAILEQLKSKK"
     gene            23680..24723
                     /gene="plsX"
                     /locus_tag="VF226_01390"
     CDS             23680..24723
                     /gene="plsX"
                     /locus_tag="VF226_01390"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7072672.1"
                     /translation="MPAQRIIALDAMGGDHGPEVVIPGADIALVRHPQLEFRFFGNEA
                     QIAPVLARYPKLAARSTVVHTEVVVKSDEKPSQALRRGRGSSMWLALDAVQNGDAGVA
                     VSAGNTGALMAMAKMILKPVANIERPAIAALWPTVTGECIVLDAGANIGASARQLCEY
                     ALMGAAMSRALYHIEKPSVGLLNVGVEEIKGVDEVRQAHAWLKEITDLPIDYRGFVEG
                     NQIGQGAVDVVVVEGFAGNIALKTAEGTARQIAQYIRAAMSRSLMSRIGAFLARGAFA
                     VLKEKMDPRRSNGGTFLGLNGIAIKSHGGTDAVGFASAIDLGYDMAQSGLLARLTADL
                     LSFHGKLDAAVEK"
     gene            24821..25798
                     /locus_tag="VF226_01395"
     CDS             24821..25798
                     /locus_tag="VF226_01395"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7072673.1"
                     /translation="MAVIRSVFRGVGAYLPKRVLTNADMSKIVDTSDEWIRERTGIRS
                     RHIAADGELTSDLGIAAARQAMVRAGIDPVDVDLVICATATPDRTFPATAVRIQAELG
                     ITKGAAFDMQAVCSGFVYALATADNFLKAGQFKRALVIGAETFSRILDWSDRSTCVLF
                     GDGAGAVVLEAQAQRGTREDRGILGTRLRSDGRFEELLYVDGGPGSTRTTGHLRMNGR
                     EVFRHAVQKISGVIEEMLLMTGYAADEIDLFVPHQANARILDGVARKLGVSPEKIIVT
                     LGEHGNTSAASIPLALNQAFEQHKLEEGSLVLMEAMGGGFTWGAALARW"
     gene            26000..26323
                     /locus_tag="VF226_01400"
     CDS             26000..26323
                     /locus_tag="VF226_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX7072674.1"
                     /translation="MGGKTLTRADLAEAVVNKVGLPRNESQELVEAVLHEIASSLARG
                     DTVKLSSFGSFGIRQKGQRVGRNPKTGQEVPITPRKVLVFRPSNIMKDRINAGQVRRR
                     KAAAE"
     gene            26377..26901
                     /locus_tag="VF226_01405"
     CDS             26377..26901
                     /locus_tag="VF226_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7072675.1"
                     /translation="MSVTKSAEAFRTISEVADELEVPKHVLRFWEQKFPQIRPMKRGG
                     GRRYYRPEDLDLLRGIRHLLHLEGYTIRGVQKILRTQGVDEVKRCWMEPSNPIADAGE
                     GAAKTKSKAKSKSKAAPGRKQGQASAKKSSASHLPEDVLRSLQTALSELEACRRILTA
                     PVADQTSWSQSAEQ"
     gene            26898..27671
                     /locus_tag="VF226_01410"
     CDS             26898..27671
                     /locus_tag="VF226_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072676.1"
                     /translation="MSGAPAGVELWFLDVARAGSALAAVERSTPRLSADELEWTAEPG
                     RNGETRRHYFRICLRLLLERFVGPRLRQVPFRTSPQGKPQLPWDAGVMFSLSHSGPYG
                     LIAIADREVGVDIEQDRPVNFPLDRQDAMLAAAGALSSAPTDSPLTILQAWVRLEAWS
                     KARATGIGVLLHDLGIKGPEWAARGPARDFGGIAASLARQEGFVLYDIALPRPLHAAL
                     ATKAGLALPPLRIVPADLSSLEALGASQPEPSSPSDPNG"
     gene            27707..27784
                     /locus_tag="VF226_01415"
     tRNA            27707..27784
                     /locus_tag="VF226_01415"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27742..27744,aa:Pro,seq:ggg)
     gene            complement(28318..29193)
                     /locus_tag="VF226_01420"
     CDS             complement(28318..29193)
                     /locus_tag="VF226_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX7072677.1"
                     /translation="MSTLYQMLGLPEGADQHQVKAAFRKLARCLHPDVNHSAAAEQFF
                     KEVTRAYKTLVDPVARAEYDRALVRQQTSERRRLRQFCMTATAAGVLSASFALLVLTW
                     AQRPGLSSSTPMGRAGTAVAVPDATEVPGRGANWSVYRNDRFRFAVGFPADVFSVSNF
                     ADDNSAVLTSEDDRAELRIFAARNVTGMTLGSYRSSLLKTRYAGVRLDHTPKGKLWFV
                     LSGSRGDRVVYEHVSFSCDGGSIYGWQMVYPAEERTIFDLIADEVARHTTTTSLRCGA
                     LPPIPRRKPLNQRKS"
     gene            complement(29506..29886)
                     /locus_tag="VF226_01425"
     CDS             complement(29506..29886)
                     /locus_tag="VF226_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7072678.1"
                     /translation="MVSVLIVEDEPVLALEMENALQKAGFAIAGIAGSCLKALAVLEC
                     RDCDLAVLDANLRGSCVEPVVAALQARGKPYLLISGYDRTHLASGLREAPFLPKPFGS
                     DALVRALNNLRVQAKTQNHMHPSC"
     gene            complement(29886..30629)
                     /locus_tag="VF226_01430"
     CDS             complement(29886..30629)
                     /locus_tag="VF226_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX7072679.1"
                     /translation="MQSDGQLRAQRRRSGRLFYFGGRGYHRPKRAAEQNRFLLREVNH
                     RAKNLLAVVQAVARQTAGENDPKVFAERFAARLSCLAACQDLLVRSNWRGVSAGELVR
                     SQLAHFVGLVGTRILLDGPTLRLSAAAAQTIGMALHELATNAGKHGALSDAQGIVEVT
                     WNLAGSDEAPEFVMCWHERNGPRVAPPERRGFGHTVIVRMVEHALDAAVSLEHDPAGL
                     VWRMSAPAQLVLDPPSNLDTSGDGSVGEP"
     gene            complement(30545..31933)
                     /locus_tag="VF226_01435"
                     /pseudo
     CDS             complement(30545..31933)
                     /locus_tag="VF226_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
BASE COUNT         6188 a        10099 c        10418 g         5618 t
ORIGIN      
        1 tgcttcggcg cctgctcggc ggctatcctc tggaacggcc agctcacctg gcggtacgag
       61 gagatggcga ccggacaagc cgagcggctc atccccatga tcggcgaggt tctggatcaa
      121 tccggagcga gtctgcgaga cctggacacc atcgccgtca ctgaggggcc tgggacgttc
      181 acgggaacac gcgtcagtgt cgctgccgcc cggggacttg cgctggcaac aggactgccg
      241 atccgcgcga ccacgagcct gcacgtgatg gcatatcgag ccatttcgga gcttggcgaa
      301 gctgcggccg gatcagtttt agccgtgtgc gtcgatgcac gcggcgggaa ggtgttcgtt
      361 caggttttcg aactgcaagc caccgaacct ctgactcaag ccacagtcat ggcgccggac
      421 gctgcggctg cgctcgaggc gcctgctccc ctgctctgtg tcggcagtgg cggactttcg
      481 gtcagcgagg cagcccgccg ggccgggcga gaggccgaag caaaactgcc aacattgcag
      541 ccggacgccc gctacctcgc ccgccttgcc gcgtcgctac ctgtgcgcga tccgctcgtg
      601 cccctttatc tgcgccctcc ggacgccaag ccgcagacgg gcaaatcctt gccgcgggca
      661 tgatgacctc cgagcgacca gcaattctcg agctgatcga agcctccagc cctgaggtca
      721 atcgaatttt gagcgcgctc catgcggcgc tgttcacccc ggcatggtcc agcgacgact
      781 tcgcgtccct gcattcgcat cccggctcca tcgccctgct tgccaggtca atcagcggac
      841 gacgcccgat cggcttcatt ctaggccgcg tcgttgtcga cgaggcggaa atcctgtccg
      901 tcggggtttt gccagaggct cagcgtcaag ggatcggctc ccgacttgtc ggcgagttcg
      961 ccagactggc agccgaacgc ggagcaacgc ggctcttcct ggaggttgcg gcggataatt
     1021 cggtcgcccg gcagctctat cgcgcccagg gcttctgcat cgtcggccgt cggcccggct
     1081 attacgagca ccccgcgaaa gccccaaccg acgcgatcat catgtcaaag ctcatcggcg
     1141 gctgacccat gggtggacgc gttacacgcg gctcatctat aagcgagaac aaagacggaa
     1201 aaggtccgtc atggaggggc attggctgaa taggatggcc ggcgcagagg atcagtcccc
     1261 gagccgcatc gaaaggcttt gcagcgagcg cgggctgcgt atgacgggtc agcgccgtac
     1321 catcgcacga gtcctcacgg aagcagagga ccacccggac gtggtcgagg tctaccggcg
     1381 cgcccaccag atcgacccgc ggatctctct ttcgaccgtc taccggaccg tcaagctgtt
     1441 cgaggaaaat ggcattctcg agcgccacga gttcgcggcc gggcgccgtc gctacgagga
     1501 ggccacccgc gagcatcacg accacctgat caaccttgaa acaggccagg tcatcgaatt
     1561 ccgcaacgag gacatcgaga agttgcagga acgcgtcgcc cgggagctcg ggttcgagct
     1621 ggtcggccac cggctcgagt tgcacggcat accgttgcgc cgcaaaaaga gcacgtgacc
     1681 gagagccgat cttcggcatc ggtcgttgaa cggcaaatga gaaccgaccc gacgccgagc
     1741 tcaaatcgcc gaggtgcgcc gcgggcagcc gccatccttg ccggatttgc cgggctgacc
     1801 ctgccactca tgcccgtgca ggccggcctc cttcggatct cgccgcacta cgcgcgcaaa
     1861 tttccgcact ggtaccatcg gcaggtttgc cgcctgctcg gaatcaggct tcacgtcgag
     1921 ggcaagattg cggagaacac cccggttctc gtggtcgcca atcacgtctc ctggctcgac
     1981 atccccgtgc tgtcggcggt cgcgcccgtc tcgttcgtcg caaaaaagga ggtcggaagc
     2041 tggccgttcg tctcatcgct cgcgcgtttg caacgcagcg tctttgtcga tcgcgagcgc
     2101 cgcaccgcgg tcggacagac gacgaccgag atcatcgagc ggctgaaatc gggcgacgcg
     2161 atcgttcttt tcgcggaagg cacctccagc gacggcaacc gcgtcctgcc gttcaaaagc
     2221 cctttgttcg cggccgtgaa accgacgctc gccaacgggc tcgccgccgc cccgggtgcc
     2281 tgcgtccaga cgcttgccat cgcctatacg catttgcatg ggattccgct tggccggtcc
     2341 ggcagatctc ttgtcggctg gtacggcgac atggaaatgg ccgctcacgc ctgggagctg
     2401 ctcaaggccg ggccactcga cgtgaagatc agcatcgacc cgccagtgtc actctccgac
     2461 tttgccgacc gtaaggaact ggcccggtat acggaggaca ggatccggca agcccttgcc
     2521 agattgctgc gtggacggaa tgattaggcc attggcttgg cgccctcatc cagacggatg
     2581 ctgagacagg caagcgtctg gacatcgtgc gtaaaatgat attgcaatgc agcaaccatg
     2641 atctacagac gcgacaatgc atttcggaac ctgagcgata gctgagctgt atagaatgat
     2701 gcggcgcaac ccatattccg gatgccgtcg ccgacggaag ctgtgaggtt cttatgcctg
     2761 gagccaggaa ggtcttcatc aagacgtacg gctgtcagat gaatgtctat gacagcgagc
     2821 gcatggccga ggcccttgcg cagcatggct attcggagac cgaggacccg gcccaggccg
     2881 acatgatcgt cctcaacacc tgtcacatcc gcgagaaggc ggccgagaag gtctattcgg
     2941 agctcggccg catgcggcag ttgaaggttg cgcgagaggt cgatggcaag aaaaccttga
     3001 tcgccgtcgc gggctgcgtc gcacaggccg aaggtgccga gatcatcgcg cgcgcgccca
     3061 tcgtcgacct cgtcgtcgga ccccaggcct atcaccgcct gcccgagctt gcggccaaag
     3121 cgcaagccga gcatcgccag ctcatcgaca cggaattccc ggacgaggac aagttcgccc
     3181 acctcccaga gcggcggggg acgaggcccg ccaccgcttt cctgacggta caggagggct
     3241 gcgacaaatt ctgcacgttc tgcgtggtgc cttacactcg cggcatggag atctcgcggc
     3301 ccgttgtcca gatcaccgag gaggcgcgcc gactcgttga gcagggcgtg cgggaaatca
     3361 cgctgctcgg ccagaacgtg aacgcctacc acggggaagg acccgacggg cggacatggt
     3421 cactcgccaa gctcctgcgg cacctgtcca gcatcgagca gcttgaacgt ctgcgctata
     3481 cgacgagcca cccgcacgac atggacgatg agctcatcga agcgcatcgg gatttgccga
     3541 agctgatgcc ctacctgcat ctgccgttcc aggcggggtc ggaccgcatc ctcgccgcga
     3601 tgaaccggaa gcatacggcc cgggattatc tgcgcatcgt ggagaagatc cgcgccgcgc
     3661 ggccggacat cgcgctttcg acggacatca tcgtcggctt cccgggagag accgaacagg
     3721 agttcgagga tacgctcaag ctggtgcagc aggtcggatt tgcgcaggcg ttctccttca
     3781 agtacagccc gcgacccggc accccggcgg ccaacatcga ggaacaggtg ccggaggagg
     3841 tcaaggccgc ccggctcgct cggctgcaag agctgctgca ggcgcagcag cggcagttca
     3901 atgcccgctg cgtgggcagg gtgttcccgg ttctgttcga gaagcttggg cgcaaggccg
     3961 gccagctcgt cgggcgctcg ccctaccttc aggctgtcca cgccgaggct gagcccagcc
     4021 tgctcggccg cattctgccc gtcgaaatct cggatgccag caccaacagc ctggccggag
     4081 tcgtgcgata gcaggtcgct ttttgttcgc acgtgctgga cgggtgtcac atctccacta
     4141 cataggctag tatagtgaca gcggtagccc cgagtcgtgg gttggccgca cgggagagat
     4201 tgcattttga acgcatatcg cggggcagca gcttccggag ccggcaggcc gacgagggcc
     4261 tcctctgcca ggaagcgtct ggtcgtcccc ttcgaaagta accgccacct cgccgagctg
     4321 ttgggcgagt acgatgcgca tctcgcgctg atagaagatc gcctcggcat cgaggcacac
     4381 gctcacggca acgtcgtcat cctgtccggc tcggagccgg cgtgcaacat cgcgcgcaac
     4441 gtgctcgagc agctttacgc gcgagtcgcg cgcggcgagc aggttggacc cggtgatttc
     4501 gacgggctga tccggcatgg ccgggaggag aagcccggca agtcgccgga tggccaggct
     4561 cagatcacga ccaggcggcg cgtcatcaag gcgcgcacgc cgacgcagag ccaatatatc
     4621 cgcggcctcg aaagcacgga tctggtattc gggctggggc cggcgggcac cggcaagacc
     4681 tatcttgcgg tcgcttatgc cgcctactgc ctcgagcgag gcattgtcga gcgcatcatt
     4741 ctttcgcgcc cggcggttga ggcaggcgag cggctgggct ttctgcctgg cgacatgcgc
     4801 gagaaggtcg atccttatct gcgccctctc tacgatgcac tctatgacgt gctgccgccg
     4861 gaaaaggtgg ggcgagacct ggaaaccggc gtgatcgaga ttgcgccgct ggccttcatg
     4921 cgtggccgta ccctctcaaa cgcctacatc attctggacg aggcgcagaa tacgaccagc
     4981 atgcagatga aaatgttcct cacccgcctt ggtgagaact cgaagatggt cataactgga
     5041 gacccgagcc agatcgacct gcccgttggc cagcggtccg ggctggagga agccgtcagg
     5101 ctgctggcgg gaattcaggg gatcgaggtg gtccggttca cgtcctcgga cgtggtgcgg
     5161 cgggatctgg tcgcacggat cgtcgaggct tatgatcggg gccaggctgc cgaagccaag
     5221 cgcgaacgac atgaacgaga cacttgaaac cgtgatcgcc gagccgccgt ccgatggcgg
     5281 cgatgatgac ccggagcctc caggacggtt gcagctcgat atcatcatcg aagacggtaa
     5341 ctggggcgcc ctcgcgccag tcgatgaggt gttcggcccg gttgcagatg cggtagctcg
     5401 aaggctgcag ctcgactctg ccgaagccgc gatcgccctg agctccgatg agcgtgtgcg
     5461 agccctcaat ctgacttatc gcggcaagga caagccaacc aacgtgctct ccttcccagc
     5521 cggcgaggat ggtctgccgc ccggatcgcg acgccacctc ggcgacatcg tgcttgcggc
     5581 cgaaacagtc gcccgggaag ccgcagctga aggtaagcca ctccatcacc atctgcagca
     5641 tctcgcggtc cacggactgc ttcatctgct cggctacgat cacgagaccg aggtggaagc
     5701 tgccgagatg gaggcgctcg aggtcgaaat cctcgggcaa ctgggggttc cggatcccta
     5761 tgcccccgaa tcactttgac gcgtgagaag caacccgttg agcattatga gcatcgacgt
     5821 cgacccatcc agaagcgaac agaaatccga cgacggaagt gaaggccaga gttggctttc
     5881 cagcttgaag ggcctgctgc ggctctccgg aaccccgaca tttcgcgatg cgctcgagaa
     5941 cgccctgaag accggaacca agaccgagga cgccttcagc gcggaggagc gccgtatgat
     6001 gctgcggctc ctccacttcg ggcggttgcg ggtcgatgac gtgatggtgc cccgtgcgga
     6061 catcattgcg atcgacgaaa gcgaaacgct gggcgaattg ttcgcgactt tcgaggaggc
     6121 cggcgtctcg cgtattcctc tttatcacga gacgctcgac gacctgcgcg gcatggtcca
     6181 catcaaggac ctgatgagct ggctgtacag cgaagccgct gtcctttccg aggagagcga
     6241 cggcgaggag gccgaggcgc aaggcggcac gcgcagatcc aatggacaca acggccaggc
     6301 gaaaccgagg ttgcgcgcgc tcgacttctc ccgcgtcgac ctgacacggc ctctggctgt
     6361 cgcgaaaatc cggcgtccgg tgctcttcgt tccgccgtcc atgccggcga tgaacctgct
     6421 catccggatg cagaccacgc gcatccacat ggccttcgtg atcgacgagt acggcggcac
     6481 cgacggcctc gtcacgatcg aggaccttgt cgagcagatc gtcggcgaca tcgaggacga
     6541 gcacgacgag gccgaggagg cgaacatcaa gttcgacccg aagtccggcc ttgtggcctc
     6601 cgcccgcacc cccgtgcagg agttggagga atatcttggc atcaagctgc tgaacaacga
     6661 cgaggaagag gagatcgata cgctcggcgg cctcgtgttc gcgctcgtgg gccgcgtgcc
     6721 ggcacgcggt gagctggtgc agcatccgtc aggcgtcgag ttcgaggtgc tcgatgccga
     6781 cgcccggcgc gtgaacaagc tgaaaatcca caccgatcgc gcgccgaagg ccggtgacgg
     6841 cggaagcgca gccggccagc tccgatcctg aatgtcgcgc gtagggggca gcgggccagc
     6901 gccagcgctg atcagcagct tggatgggct ccatcgcgcc gcttgtttca tacgaaagct
     6961 gacgggctgg tggcgtattt gtgtcgcatt catcagcggc ggcctgtgcg cgctggcgat
     7021 ggcgccgttc ttcctctggc ctgtcctgtt cctctccttc cccgttctca cgtggctgat
     7081 cgacggcgcc ttggatccag gaaagcagcc ggcggaatcg gagatccggt cggctaccgc
     7141 tcacgcgcgc aacgccgccg tcgtcggatg gtggttcggc gcgggctact tcctggccgg
     7201 actgttctgg atcggcgagg cctttcttgt cgaggccgag cgctttgcct ggctgatgcc
     7261 attcgcggtc atgctgatgc cggcggggct ggcgctgttt tatgccgcag cgactgctgc
     7321 cgccgccctc ctttggcgcc cagggctggt gcgcgtgctc gctttggcgc tgaccctgtc
     7381 tgccgccgag tggctgcgcg ggcacattct gacggggttt ccctggaacg tgctcggcta
     7441 cgcgctgacc tggccactgc cattgatgca atcggctgga ctgttcggaa tttacggcct
     7501 gacgttgctg gccgtcctca tctttagtgt gccggcagtc gctctcgccg atgcggcggc
     7561 cgggcatgga cgtgtccgga ccggcctggc cgccgcattg ctcgccatgc tgccgctgcc
     7621 tctggcgtgg gcctatggct cttaccagct tgccgagccg cgacccgcgc cgatcgaagg
     7681 cgtacgcctg cggctcgtgc agccgagcgt cccccagcgc aacaaatggc ggccggaaca
     7741 ccagcgcgac atcttcatcg atcacctcaa cctctcccag cgcgccccgg acggcagttt
     7801 cgacgggctc gctgggatca cgcacgtgat ctggcccgag gccgccatgc cgtttctgcc
     7861 gctcgatacc ccggaagcat tggctgcgat cggcagaatg ctgcctgaag gcacgcaatt
     7921 gcttgcaggt gcgctcaggg ccgacggagc cgtttcgccg gcacaaggca ggcgcgcttt
     7981 caacagcctg atcgcgatcg attccgatgg ccggccgacg gccatttacg acaaggtgca
     8041 tctcgtcccc ttcggggaat acctgccgct gaagccgttg ctcgagcggc tcgggctgca
     8101 gaagctcgtc ggttggcggg gcgggttttc cgtcggaaga tccccacggc cgctattcga
     8161 ggtggccgga ctgccaccgg tgagcgctct tatctgctat gaagcgatct ttccgggcgc
     8221 tgtcgtcgag ccgggccagc ggccgcgcgc cctcatcaac atcaccaacg acggctggtt
     8281 cggtgagacg accggcccgc accagcattt tcaccaggcg cgtgtccggg ctgtggagga
     8341 aggcttggcg cttatccggg tcgccaacaa cggaatcaca ggcgtgatcg acgccagcgg
     8401 gcgagtcctc gcgagtctgg gcctcaacga gcgcggggtc atcgacaccg atctcccggg
     8461 tccgaagccc cctccgctct atgctcgatt cggcgatgcg gtgttcgcag ccctctggct
     8521 agccggcttt tgctggctgg cgggctcctt gtggcgcaag aagtccactg ctcactaagc
     8581 cgacgatttc agtgcggagt cgaaatccgc tacttacacc tcttgttccg gttgacggac
     8641 gctttgcctg cacctacgaa ttgcgcgggg aaaagagttt tgtcgccaca gggtggggag
     8701 aataggggca tggccagaca agatgatgca gttgccgagc atgacttcga cgtcgtcgaa
     8761 aagggctcgc ggcgggcaaa cccgcttgac ctgcacgtgg ggagccgggt gcgactgcag
     8821 cgcatgctgc tcggcatgag tcaggagaag ctgggtgagc agctcggcct caccttccag
     8881 cagatccaga aatacgagaa aggcatcaac cgtatcggtg cgagccggct ttacgagctt
     8941 gcccgcgtgc tcggcgtcag cgtccagttc ttctacgaag atgcacccgg gacaaccagc
     9001 gaatatcggt cgcagccgaa ttcggcggcc ggtggccttg cggagcgggc ggcggatcgc
     9061 tacgtggtcg atttcttgag cagtcgtgaa ggcgtcgagc tcaacaaggc ctttgtaaaa
     9121 atttcagatc cgaaagtccg gcgttccatt gtcgatcttg tccgtacttt ggccggcgaa
     9181 gaaaaggatt cttaaaagct gcagtgtgcc gcgatagccc ggacattggc tccgggcgac
     9241 gcggcttgat tcccttcgca aagactgtaa agaagcgcag cgacctgcgg ctccactcag
     9301 tttgtcgggg acacccgtgt cacgccaatc ttacctgttc accagcgaat ccgtctccga
     9361 gggccatccg gataaggttt gtgaccggat ttccgacgag atcgtcgacc tgtttttccg
     9421 ggaagcgccg aaagccggcg tcgatcctcg ggacgtgcga gtggcagccg agacgctggc
     9481 cacgaccaat cgcgtcgtga ttgccggcga gacccgcggc cctgcgacca tcacgaagga
     9541 tctcgttgcc catctcgcgc gcatggcgat ccgtgacatc ggctacgagc aggaaggctt
     9601 ccactgggag aaggcagcca tcgaggtgct gctgcatccg cagtcggcgg atattgcgca
     9661 gggcgtcgac gctgataagg aaaaggacga aggggccggc gatcagggca tcatgttcgg
     9721 ctacgcctgc cgggaaaccc cggagctcat gccggcgccc atctattaca gccacaagat
     9781 tctcgaagtg ctggccgatg cccgcaagcg caaggaaggc gatgcggcgc tgctcggccc
     9841 ggactccaag agccaggtca cggttcgcta tgagggcggc aaggccgtcg gtgttacgca
     9901 aatcgttctc tcgacacagc acctcgcacc ggagctgact tccgcgcagg tgcgcgacat
     9961 cgtcgagccc tatatccgga agattttgcc cgagggctgg atcgacgcga ataccgcctg
    10021 gcacgtgaac ccgacaggaa agttcgtgat cggaggaccc gatggcgact gcgggctgac
    10081 cggccgcaag atcatcgtcg acacctatgg cggtgcggcc ccgcatggcg ggggtgcctt
    10141 ctccggcaag gacccgacca aggtcgaccg gtcggcggct tatgctgcgc gctatctcgc
    10201 caagaacgtg gtcgccgcag ggctcgcgga caggtgcacg atccagctcg cgtacgccat
    10261 tggagtcgca gagccgttgt ccatctactt cgatacctat ggcaccggcc aggttccgga
    10321 ggagcagctt gagaaggcgg tgcgcaaggt gatgaagctg acgccgcgcg gcatccgcga
    10381 gcacctcggc ctcaacaagc cgatctatgc gcgcaccgct gcttacggcc atttcggccg
    10441 caagccggac gcggatggcg gcttctcctg ggagaaggtc gatctggtcg atgccctgaa
    10501 gagcgccgtc ggctaggctt cgatttccga aatcctttga tcggccgcgg ctcatccccg
    10561 cggccgattg gctttcaaga cgcggacgag atgggcgagt tgcagaggag accgcgcgag
    10621 gacatccgca gctacgggcg gcggcatggg cggaaattca gcgcccgcca ggagcgcctt
    10681 ctggccgagc aattgccacg cgttgccatc gacctcgcct cgcccccacc tgcccacatc
    10741 gtagacctgt ttgcctgccc ggtccgcgat gtctggctcg aaatcggctt cggtggcggc
    10801 gagcatctcg tctggcaggc cgagcacaat cccgatgtgg ggctgatcgg ctgtgagccc
    10861 tatctcgacg gtgtggtgaa ggtgctggcc gcaatggagg agaagggcct ccgaaacatc
    10921 cgcattctgg ccgacgatgc ccggccgctg ctgcgctggt tgccgcaggc atccatcgga
    10981 cgcgttttca tcctgtttcc ggacccctgg cccaagacgc gtcaccgcaa gcggcgcctc
    11041 gtctcggcga gcttcctcga aacgctagcc aaagtgatga agcccggcgc ggagctgagg
    11101 attgcgacgg acatcggcga ctacgctcgc acaactctcc tcgccttccg cgctacgccc
    11161 gcgttcgaat ggctggccga agcgccgtcc gactggcgag agcggacatt tgattggcca
    11221 ccgacccgct atgagcagaa ggcgcttgca gcaggacgtc gccctaccta tctacgattt
    11281 ctgcgcactt gagcagagcg tagcggggta aatcgaacaa aattgccgca tggacaagag
    11341 gcaagctgcc cgattttcgc catttgcgct tgacctcgcc agtacctatc tcatatgctt
    11401 ccaatgctca tgatcatctc aactctgaga gtgggccccg ccgggtccgc tctttttgtt
    11461 ttatgctgct tgtgagcaac ttgattgcag gtttcgaggc gacatggacc agcgggatga
    11521 gggccaaagt gcacggcgct tcattcgcga gaccggcctt gccgcgcaga tcgccgagct
    11581 cgtcgagccg acattggaag acctcggctt ccggctggtg agggtgcaga tccacggccg
    11641 ggacggccag acggtccaga tcatggcgga gcgtccgaac ggcacgatga cgatcgagga
    11701 ttgcgaagcg atctctcgac agctatctcc tcttctggat gcgcacgatc ccctgccggg
    11761 cagctaccgg ctggagattt cttccccagg gatcgaccgg ccgctcgtca gaccgagcga
    11821 cttcgaggac tggtcgggct acgaagccaa gatcgagctg aagcagcagg tgggcggacg
    11881 caaacgcttc cgcggcgttc tcgaagggtt cgaggacggc gaggtgcgac ttgaatgcac
    11941 gctcgatcag cagggcaccc aggtgctggg ctttccagtg gatctggttg cagacgccaa
    12001 gctggtgctg acggatgagc tcatcagaga ggcgctgagg cgggcgaaga agagttcgaa
    12061 gggtgatggc ggctcagggc cgccgcagga agattaagcg acagaggtgc aagggatggc
    12121 cgttgcagtc agtgcgaatc ggttggagct gctgcaaatc gcagacgcgg ttgcgcgcga
    12181 gaagtccatc gatcgcaaga tcgtcatcca ggcgatggag gacgcgatcc agaaggcggc
    12241 caagtcgcgc tatggcgcgg acaacgacat tcgctgtgag atcgacccgc gcacgggcga
    12301 cacgcgtctg tcccgcgtgc tggcggtggt cgagaaagtc gagaacgatg ccaccgagat
    12361 ttccctggag gaagccaagc gccgcaaccc cgaggcaaag atcggcgatt tgatcgccga
    12421 gccgctgccg ccgctcgatt tcgggcgcgt agcggcgcag aacgcgaagc aggtcatcgt
    12481 acagaaggtc cgcgaggcgg agcgcgagcg gcagtacaac gagtacaagg accgtgtcgg
    12541 cgagatcgtc aatggtacgg tcaagcgggt cgaatacggc aatgtgatcg tcgacctcgg
    12601 ccgggccgag ggaattatcc gccgcgacga aatgattccg cgcgagaaca tgcgttacgg
    12661 cgaccgcgtt cgtgcctata tatacgatgt acgccgcgag cagcgcggac cgcagatttt
    12721 cctgtcgcgt gcgcggccgg agttcatgtc caagctgttc gccatggagg tcccggagat
    12781 ctacgatggt gtcgtagaga tcaagtcggt tgcccgtgat ccgggctcac gcgcaaagat
    12841 tgccgtgatc tccaaggaca gctcgatcga tcctgttggc gcctgcgtcg gaatgcgcgg
    12901 ccagcgcgta caggcggtgg tgaacgagct gcagggcgag aaggtcgaca tcatccagtg
    12961 gcggccggac gcggcggagt tcatcgtcaa cgcgttggcc ccggctgagg tgaccaaggt
    13021 cgtcctcgac gaggactcga acaggatcga ggtcgtcgtt ccggagagcc agctctcgct
    13081 ggcgatcgga cgccgcggcc agaacgtgcg gctcgcttcg cagctcacgg gttgggatat
    13141 cgatatcctg accgagcagg aagagagcga gcggcgacag aaggaattcg cggagcgctc
    13201 gcagatcttc atggaggcgc tcgacgtcga cgaagtgatt gcccagctgc ttgtgaccga
    13261 gggcttcgct accgtcgagg aggtcgccta cgtcgacctc agcgagatcg cccacatcga
    13321 gggcttcgac gaggatacgg cagaggagat ccagacccgc gcccgcgagt atctcgagcg
    13381 catcgagcag gagcgggacg cgcggcgcaa ggagctcggg gtctccgacg acctcgcaca
    13441 ggtccctggg atgaccactg caatgatggt cgccctgggc gagaacggca tcaagacgct
    13501 cgaggatttc gccgactgcg cgaccgacga cctcgtcgga tggaccgagc ggaagaagga
    13561 gcgggacgcc gagccggtgc ggcacaaggg cattctcgac ggcttcgacg tgggcaggaa
    13621 ggaagccgag gacatgatca tggcggcccg catcatcttg ggctggatca accccgaaga
    13681 cctcgagcag cccgccgaag aggcggtcga gggggcagag gcggtcgagg agacgaccgg
    13741 cgaagccggg tcgagcgcgt gacagcggcc atgaccagga gcggacagag cacgaatggc
    13801 ggtgcggttg gacagcgcag aaagaccagc ggcggagcga acggctccgc tgcggacctg
    13861 cgtcgtaacc cgcgatcaga aagcgcctga ggagctcatc cgcttcgtac cgtcccctga
    13921 cggtgagatc gtgccggacg tggcgcgccg ccttccaggc cgcggagtct gggtgactgc
    13981 cgaacgcggt tacgtggccg aagccgtcaa gcgcaaagcg tttgcaaaga gcttgaagcg
    14041 gcaggtgtcg gttccacccg atcttcccga cgtggtggag cggctgcttg tgaagcgcgc
    14101 catggatgcg ttgagcctcg ccaacaaggc ggggctcgtc atcacgggct tcagccgcat
    14161 agaagcggca attgctgcag gcactgttgc cgcattgctg catggccaag aagccgcagc
    14221 agatggcgca ggcaaactgg accggcgatt tcgtgcggtc tgtaagcaaa ttggcaagcc
    14281 tgcacctatc attcaggaat tgaccgtcga ccaaatgagt ttggccttgg ggcggtcgaa
    14341 tgtggtacat gctgcattga gcgcgggcgg cgccacgacg aatttcctga acgaggttgg
    14401 ccgagtgacc cgatatcggt cgggcgcgtc ggcttcgaac accaggtatg cggccgacca
    14461 aggggccgtc taaggatttt tggaacgggc aaggtatgag cgaaacgaaa gaaaccaccg
    14521 aaaagaccgt gctgacaggt acgcgcaagc cgctcagcct gaagcggacc atcgagtccg
    14581 gccacgtgca gcagaagttc agtcacggcc ggtcgaagtc ggtggtggtc gagaaaaaga
    14641 gaaaacgcac catcgcgggc caagagccgg aggagacggc ggcggctccc gtgcaggtgc
    14701 gcccgcgccc gcatgtcgcg caacagcggc cggccccgaa ggccgagcag caacagcagc
    14761 cgaagatcct gcgtacgctc tccgaggagg agcagcaggc cagagcgcgg gctctcgccg
    14821 ccgcgcaggt gcgtgaggcc gaggaacagc ggctccgcga ggagcgcatg cgccaggagg
    14881 ccgagcagcg ccgccgtgag gaggcggagc gcaaggccgc tcagctcact gaggctgccg
    14941 ccaaagaggc agagcccgag cgggcggagc ccgcagccga agcggagacc gcggcccagc
    15001 ccgaggcacc tgccatggca gaaaccccgg ctgcgccgga ggagcccgca gcgcctgcgg
    15061 cagccgccga ggcagccgcc gctcccgaag agcccgagac acctgaggtt ccgcaggtct
    15121 tgccgcagcc catcggcatg acgcgtccga tcatcgtctc gcgtccggag cgcagccagc
    15181 cgaaggccgc cgagacggcg aagaaggcgg aggtcgaggt tccgaagcgc ggggccaagg
    15241 cagccgcgct ggccgagatc gaggaagagg aaggccgcac caagaagaag ggcggcaagc
    15301 tcgtccgcgc gccgctcaag ggcgccgacg aatcgcgcgt tcgcggcaag ctgacgatca
    15361 ccaacgcgtt cgacgagcgc cagcgcgagc ggtccctcgc ctcactgaag cgccgccgcg
    15421 agcgcgagag gctgcgcgcc atgggcatcc cgcagcagcg cgagaagatc gtacgcgagg
    15481 tcgtcattcc ggaagcgatc acgatccagg agctctccaa ccggatgtcg gagcgcgcgg
    15541 tggacgtcat caagttcctg atgaagcagg gcgcgatgca caagatcacc gacgtgatcg
    15601 acgcggacac ggccgagctc atcgtgcagg agttcgggca tacgccgaag cgggtttcgg
    15661 aagccgacgt ggaggaaggc ttcatcggcg ccgaggacgc gcccgagacg ttgcggccgc
    15721 ggccacctgt ggtcacgatc atgggtcacg tcgaccacgg caagacgtcg ctgctggacg
    15781 cgctccggca gacgaacgtc gccgccggcg aggcgggtgg catcacgcag catatcggcg
    15841 cctatcaggt gacgacaccg accggcgagg tcgtaacctt catcgatacg ccgggccacg
    15901 aggccttcac cgccatgcgt gcccgcggcg ccaaggtgac cgatatcgtc gtgctggtgg
    15961 ttgcggccga tgatggcgtc atgccgcaga cggtcgaggc catcaatcac gcgaaggccg
    16021 ctggcgtgcc gatcatcgtc gcgatcaaca agatcgacaa gccggacgcc gacccgaccc
    16081 gcgtgcgcac cgagctgctc cagcacgaga tcgtggtcga aagcatgtcg ggcgaggtgc
    16141 tcgaggtcga ggtttcggcc ttgaagcgta tcaacctgga caagctgctc gaggccatct
    16201 cgctgcaggc cgagctgctc gaactcaagg ccaacccgaa tcgctccgcc gagggcttcg
    16261 tcatcgaggc caagctggag cggggacgcg gtccggtggg cacccttctc gtccagcgcg
    16321 gcacgctgag agtcggcgac atcgtcgtgg ccggcacctc ctggggccgc gttcgcgcgc
    16381 tgatcaacga caggggcgag aacgtgaagg aagccgggcc atcggtgccc gtcgaggtgc
    16441 tgggcttcga ctccgcgccc gaggccggcg accagttcgc cgtggtcgag agcgaggctc
    16501 gtgcccgcga gatcacggat taccgcatcc gtaagcgtcg cgacacgctc ggctccgttg
    16561 gcaccgcccg gacgctcgag cagatgatgc agcagctcaa ggacgccgag aagaaggagt
    16621 tcacgctgct ggtcaagggc gacgtgcagg gctcggttga ggccatcgcc ggtgctttga
    16681 agaagatctc gaccgacgag gtcgaggcgc ggatcgtgca cgcgggcgtc ggcggcatca
    16741 ccgagtcgga tgtggcgctg gcccatacgt cgggagccgt gatcatcggc ttcaacgtgc
    16801 gcgccaacgc ccaggccagg gccgcggcgg agcgcgacgg ggtcgagatc cgctactaca
    16861 acatcatcta cgatctcgtg gatgacgtga aggcggcgat gtccggcctg ctggcaccga
    16921 ccctgcgcga ggtcttcctc ggcaatgccg aggtcctcga ggtgttcaac atcacgaagg
    16981 tcggcaaggt cgccggctgc cgtgtcaccg agggcaaggt cgagcgcggt gcaaaggtgc
    17041 gcctgatccg cgacaacgtg gtgatccacg agggcacgtt gtcctcactg aagcggttca
    17101 aggacgaggt gaaggaggtc ccggcggggc aggagtgcgg tatggccttc gccaactacc
    17161 aggacatccg cgtcggcgac gtgatcgagt gcttccacgt cgagaccatc aaacggagcc
    17221 tttgatcgct cgagacgcaa ttcggccggc ggcgggcgac gcaattcgct cccgccggtt
    17281 ctcttttatg actttctcaa aggaggctgc gccggttcga acccggtcag tgacatcatc
    17341 atgccgagga atgcgccagc taggtcggtt ccttcccagc gccagctccg cgtcggcgag
    17401 ctgatccgcc atgccctttc cgagctgatg acccgcggcg aaatccatga cgacgtcatc
    17461 gccgagcacg tgatcacgat ttccgaagtg cgcatgtcgc ccgacctgag gctcgcgaca
    17521 atctacgtgc tcccgctcgg cggcgtcgat acggagcccg tactcgccgc gctggagcgc
    17581 aacaagcgct tcatccgcgg ggtggtggcg cgcgcggtca atctcaagtt cgcacccgac
    17641 atccgcttcc gcaaggacga gagcttcgag cgcggcgagc gcatcgaccg gctgctcgac
    17701 agcctcaagg aacgtgaata acgccccatc ccgattgcga agcctgtcgg ccgaccaagc
    17761 tgcttcactc ccaatccgag accaaagatg gcacgcgcga agaaaggcaa tccgattcac
    17821 ggctggctga tcctggacaa gccgcagggg atgacgtcga cgcaggccgt cgcggccgtc
    17881 aagagccttt ataatgcgca aaaggccggc cacgccggaa cgctcgaccc cttggcgaca
    17941 ggcatccttc cgattgcgct tggcgaggcg accaagacct gctcgttcgc ggtcgacggc
    18001 gagaaggcct accggttcac cgttcgctgg ggcacggaga ccagcaccga cgatgccgag
    18061 ggagaggtca tccaaacgag cgaccgccgc cccactgccg aagacatcga agagattctg
    18121 ccgcgcttca cgggcgagat cagtcaagtg ccgccgacct actccgccat caagatcaac
    18181 ggcgagcggg cctatgacct tgcccgggag ggcgagaagc cggtgctcga gccgcgcccc
    18241 gtgctgatcg aggagctaag gatcgtcgaa cgactcgacg agagcacgac ggtgttcgag
    18301 gcggtctgcg gaaagggaac ctacgtgcgg gcactggcgc gcgacatggg gcgcgagatg
    18361 ggatgctacg gacacgtgat cgcactgcgc cggacacagg tcggcccgtt cggcgaggag
    18421 gtcgccgtgc cgctcgacga gctgcaggac gcgagcaggc tcgacggcgc gatcgaggca
    18481 ctgaaggcga agctcctgcc cgtcgagtcg gtgctcgacg acctgcagcg gatcagtctc
    18541 agctcggctg acgcgtcccg cgtccgcagc ggccagaatg tgctcatccg cggccgggac
    18601 gcgccgattc tcaggggacc ggcctacgcc atggccaaag gccagctcgt cgccatcggc
    18661 gagctcgcca aaggggagtt ccggccgacc cgcgtgttca atctcggctg accggcggaa
    18721 tacatcgtac cgattgcggc ggagaaacta ctcgccgttc gccaccgttg gtgtataagg
    18781 gcgctgcgac cgggccagag ggcccgtgtc ctcactgtga cccggctgga cgacatcccg
    18841 gctgtggccg cgaacaaggg ctagctgccc atcccaaatc aacgaaagga aacccgatgt
    18901 cgatcactgc ggagcgcaag caggagctca tcaaagagaa cgcgacgaag tccggcgaca
    18961 ctggctcccc cgaggtgcag atcgcgatcc tgaccgagcg catcaaccag ctcaccgagc
    19021 acttcaagat ccacaagaag gacaaccact cacgccgcgg cctgctgaag atggtaggcc
    19081 agcgccgtca gctgctcgac tacttgaagc gcaaggatca ggcacgttac caacggatca
    19141 tcgagaagct cggcattcgt cgctgatgat aaggcgggcg gccgtgttgc tgcccgccat
    19201 ttttgttgga tcggccaagt gtcgggccgg ttcgcattcc gaggccgctg caggtggcaa
    19261 accgtttcgg acaggccgtc atcgagccga tcgggtcaga tggccattcc gttcctgtga
    19321 ctgctccgaa atccgaacct tgtgccggtg tacgaacggc acaagattga gacgactcaa
    19381 aaattcgaaa gacgcaaaat gttcgacatc catcgtgtgg aaattgaatg gggcgggcgc
    19441 aagctcaagc tcgagaccgg acagttggcc cgtcaggcag acggtgccgt gctcgcccag
    19501 tatggtgaaa cgaccgttct tgccaccgtg gtcgcagcga agaccgcgcg gcctggcgtg
    19561 gatttcttcc cactgaccgt gaattaccag gagaagacct acgcggcagg caagatcccc
    19621 ggcggctact tcaagcgtga aggccgtccg accgagaagg agacactcac ctcccgcctg
    19681 atcgaccggc ccatccggcc gctgttcgtc gaaggcttca agaacgagac ccaggtgatc
    19741 gctaccgtcc tctcgttcga caacgagaat gatcctgaca tcgtcgccat gatcgcctcc
    19801 tcggcagccc ttactctatc gggcgtgcca tttctggggc ccatcggcgc ggcccgcgtc
    19861 ggccttatcg acggcgagct cgtgctcaat cccctcatcg acgagatgga aaagaccaag
    19921 ctcgacctcg tggtcgccgg cacgcaggac gccgtcatga tggttgagag cgaagcgcac
    19981 gagctctccg agaagcagat gctcgatgcc gtcatgttcg ggcaccggca tttccagccg
    20041 gtgatccagg ccatcatcaa gctggccgag actgctgcca aggaaccgtg ggacatcgcg
    20101 atcccagaca aggccaaata caaggaccgc gtgaaagaga tcgccgagga ggagctgcgc
    20161 cgcgcctact cgatgacgga gaagcagaag cgccgcgaaa cggtcgaggc gattcgccaa
    20221 aaagtgttcg aggagctgtt gccagcggac gacccaaccg acgccgttct gctcggcgac
    20281 gtgttcaaaa gcctcgagca ggacatcgtc cgtggcaata tcctccagac cggccgccgc
    20341 atcgatggcc gcgacctgaa gaccgtgcgg ccgatcatgg cgcaggtcgg cgtgcttccc
    20401 cgcacgcacg gctcggcgct cttcacacgc ggggagacgc aggcgctcgt cgtcgcgacg
    20461 ctcggcaccg gcgaggatga gcagtacgtc gacacgctgg agggcacgaa gaaggagcgt
    20521 ttcatgctcc attacaactt cccgcccttc tccgtcggcg agactggccg cataggctcg
    20581 cccgggcgcc gtgagatcgg ccacggcaag ctagcctggc gcgcgttgaa tccgctcgtc
    20641 ccgtccgcgg acgagttccc ctacacgatc cgggtcgtct cggagatcac cgaaagcaac
    20701 ggatcgtcct cgatggcaac cgtctgcggg tcgtcgctcg ccatgatgga cgccggcatt
    20761 cctctgaagc ggccggtggc cggcatcgcc atgggtctca tcaaggagga tgactcgttt
    20821 gcggtgttgt ccgacatcct gggcgacgag gaccacctgg gcgacatgga tttcaaggtc
    20881 gccggaacgg ccgaaggtgt cacctcgctg cagatggaca tcaagatcac cggcatcacc
    20941 gaggagatca tgcgcgtcgc gctcgatcag gcgcgcgagg gacgcttgca catcctcggc
    21001 gagatggcca aggcgctcgg ccaggcgcgg gccgagctcg gcgagcatgc accacgcatc
    21061 gagacgatcc atattccgac cgacaagatc cgcgaggtca tcggctcggg cgggtcggtc
    21121 atccgctcga tcgtcgagga gagcggcgcc aagatcgaca tcgaggacga cggcaccgtc
    21181 aagatcgcag cggcccgccg cgagtccata gacgctgcgc tgaagcgcat ccgcgagatc
    21241 acctccgagc ccgaggtcgg cgagatctac aagggcaagg tcgtgaagat catggacttc
    21301 ggcgcgttcg tgaacttctt cggctcccgc gacggcctcg tgcacatctc gcagctcacc
    21361 tcaaaggggc gcccgagctc cccgagcgag gtcgtgaagg aaggccagga ggtctacgtg
    21421 aagctcctcg gcttcgatga ccgcggcaag gtgcggcttt cgatgaaggt cgtcgaccag
    21481 gagaccggca aggaaatccc gccggagccg cgccccgagg gcgaagaggc agcggagccc
    21541 cggggtgaac gccgcgagcg gcgcgagcgt cgccgggacc gcgaggcgag ttgaacagcc
    21601 acaagcgtca gatcagaacg aaaaggcccc gtcggggccc tttcttttgg tcggagcggg
    21661 gagccgcttt tggtatcata gacgaccgcg ggtcgaggac cgcgaatacc ccccggctct
    21721 tcgctgcccg gaacggaccc gactcagctt ccgaaaatat tggtgcgctt gccgaggttg
    21781 cggaagatct gcttgaacag accatcctcg tacgagttgg cgggcggcgt agtgcggctg
    21841 atgaagtcga acaccttgcc gtccttcaga ccgtactcgg caatcctttc gacggtaccg
    21901 gcgcgcgtga agtagatcgc gacgacgcgc cggtccacct cctcgggcgc cagaaacgcg
    21961 acctgcttgg tcgtgctcga gatgtagtag aaggcatcgc ccgtcccgac ggagctcgtc
    22021 gtggtcggcg tgccgagcac catccggacc tgctcctgag tcatgcccgg ctggatctgt
    22081 tgcagttccg atggctggaa ggaatgcccg tgcttcgtga cgatggctgc ggagctgcat
    22141 gccgcaagga tcgcggccag tgaggccgca ccggctagca gcacgccgcg acgtgccgcc
    22201 tcacgcagtc gcactggcga tgcaccgacc ggctggccat gtttacgttt ggtcatcccc
    22261 gaagcatcaa ccccctgatc tgtgggaatc agctttgtga ttccacccgc ctccgattat
    22321 ccggaggcac gattcattac aatgcggagc ggcaagttat gcatcatcca tcgctaaagc
    22381 agcaggccgg gcaggtctct cgatgttgag ccggtggaga gcgcgagtcc gttcgaatcg
    22441 aacggcatca gagctttatg gctcgatcgt ggccgccgcc cgccgcgaac cattctattc
    22501 ggtgtatggc gtcccggata cgccggatgg tcgcttcgca atgattatcg tgcattccta
    22561 tctcgttctt gagcggctac agcatacagg cggaccgggc cagacgctga cccggcctct
    22621 cgtcgaagcg tttgtcacgg atatggacga ttgcatgcgt gagatggggg tcggcgatct
    22681 caccgtgccg cggaaagtga agaaggcagc cggcgccctc tacgagtgtg ccgccgagtt
    22741 ccgcaaagcc gagcaagcag gcagcgaggc tgctctcacg gaagccgtgc acaaaaagct
    22801 gctcccgttt gcgcctcgat ccgctgccgc cagtgtggca gcctacatgc gaagggcgac
    22861 ggccactctt gcggcttgcc cagatgcgga cctgctctcg ggccgagcta ctttcgagga
    22921 cgtttgaaac gaagcagaga atgagcgctg cgatgacatc ggagctcgag tggtcccacc
    22981 gcgtcagcga cattcccgaa acggggctcg ccgcggaacg ccacgccacg ccggaagcgc
    23041 gtgctaaggt tgctgccgag ctagaccttc ttgactgcgc gcgcctggat gcccgctacg
    23101 aggtgaagcc gctcaagcac ggacgctatc tccttcaagg cgagctcgaa gccgacgtga
    23161 cccagacctg tgttgtcacg ctcgagcccg tcgagggcca catcgtcgaa ccgttcgagg
    23221 tcgagttccg cccgggcccc ccggccgagc aggtggttga gttcaatgca ttggaggtcc
    23281 gcgacatcga accgctggaa aacggcacga ttcctgtcgg ccggatcgtc tatgagcatc
    23341 tcgcctccgc gctcaatcct tatccgcgga aggagggtgt cgctttcgag caacccgcct
    23401 ccgagcaggg cgaggaagag gcttcaggga gcccattcgc catactcgaa cagctgaagt
    23461 ccaagaagta agtgtctttt ttcgataccc cggcccggca ccgcaggctg tggcacaacg
    23521 ctgtcacccg gcgcggtcaa tgcagggctt gattaagaat tccttgtcta cggccgccaa
    23581 aggggtatgt tccgtgcgtt cgcaggcgag cgcaggcaag gctgaagcag gcgttcctcg
    23641 ccgtactctc caagggatcg gttacccagg ctaacacgca tgcccgctca gcggatcata
    23701 gcgctcgacg ccatgggtgg cgatcacggg cccgaagtgg tgatcccggg cgctgacatt
    23761 gcgctggtcc ggcatcccca gctcgagttc cggttctttg gcaacgaggc gcagattgca
    23821 cccgttcttg cccgctatcc gaagctcgcc gctaggtcca cggtcgtcca cactgaggtc
    23881 gtcgtcaaat cggacgaaaa acccagccaa gccttgcgcc gtggccgcgg ctcaagcatg
    23941 tggctcgccc tcgacgccgt gcagaacggg gatgccggcg tagcggtttc cgccggaaac
    24001 accggcgcgc tcatggccat ggccaaaatg atactgaagc cggtggcgaa catcgaacgc
    24061 ccggcgatcg ctgccctgtg gccgaccgtc accggtgagt gcatcgtgct cgatgctggc
    24121 gccaacatcg gtgcttcggc gcgacaactt tgcgaatacg cccttatggg cgctgccatg
    24181 tcgcgcgccc tatatcacat cgagaaacca agcgttggcc tcctcaacgt cggcgtggag
    24241 gagatcaagg gcgtcgacga ggtacgccag gcccatgcat ggctcaagga aatcaccgat
    24301 ctcccgatcg attatcgagg ctttgtggag ggcaaccaga tcgggcaggg agccgtcgac
    24361 gtggtcgtcg tcgagggctt tgccgggaac atcgcgctga aaacggcgga gggcacggcg
    24421 cggcagatcg cgcagtacat tcgggcagcg atgagccggt cgctgatgtc gcgaatcggt
    24481 gctttccttg cgcgcggtgc ttttgcagtg ctgaaggaga agatggaccc gcgacgctcg
    24541 aatggcggca ctttcctcgg tctcaacggc atcgcgatca agagccacgg cggcacggat
    24601 gccgtaggct ttgcgagtgc catagatctc ggctacgaca tggcccaaag cggtctactg
    24661 gccagattga cggcggacct gctctcgttc cacggcaagc tcgacgctgc tgtcgagaag
    24721 tagcgctgaa gtggcgaacc taatcagaag gcaaccggaa acgagccgag gcggatggcg
    24781 gcgatcgtgc cgctccgcgg ctcgagagta gggggacgac ttggctgtca tccgatcagt
    24841 ttttcgcggc gtaggcgcct acctgccgaa acgcgtgctg accaacgccg atatgtccaa
    24901 gatcgtcgac acgagcgatg agtggattcg cgagcgcacc ggcatcaggt cgcgccatat
    24961 cgccgccgat ggtgagctga cgtccgatct cggcatcgct gcagcacgac aggccatggt
    25021 ccgcgccggg atcgatcctg tcgatgtcga cctcgtgatc tgcgcgacgg ccacgccgga
    25081 ccgcaccttc ccggcgacag ccgtgcggat ccaggcggag ctgggcatca ccaaaggtgc
    25141 cgctttcgac atgcaggcgg tctgctctgg cttcgtctac gcattggcca cggccgacaa
    25201 cttcctcaag gcgggccagt tcaagcgcgc gctggtgatt ggagcggaga ccttttcccg
    25261 gattctcgac tggtccgatc gcagcacgtg cgtgctcttc ggcgacggcg ccggcgcggt
    25321 ggtactcgag gcgcaagccc aacgcggcac gcgtgaagat cgtggcatcc tgggcactcg
    25381 cctgaggtcc gatggccgct tcgaggagct gctctatgtg gacggcggcc cgggctcgac
    25441 cagaacgacc ggtcacctgc gcatgaacgg ccgtgaagtc ttccggcacg ccgtgcagaa
    25501 gatctccggt gtcatcgaag agatgctgct gatgaccggc tatgcggcgg acgagatcga
    25561 tctgtttgtg ccgcatcagg cgaatgcgcg cattctcgac ggcgtggcca ggaagctcgg
    25621 cgtctcaccc gagaagatca tcgtcacgct gggcgagcac ggcaacactt ccgcggcctc
    25681 gatcccgctg gcgctcaacc aggctttcga gcagcacaag ttggaggaag ggagcctggt
    25741 cttgatggaa gccatgggcg gcggcttcac ttggggcgct gcgctggcgc ggtggtaaac
    25801 ctcagcgctc gccgcagtcg ctccggatga tcctgcaatg cgggacgcct tgttccataa
    25861 cggcttttgg gttcgttgca tcgttgcaca ccggtcggga aatttctgat aaatcagccg
    25921 cttgcataag atccggattg accgtatcga ttgagaggta ttagcatctg aacgggagtg
    25981 gagtcctggg gaggatacca tgggcggaaa gacgctaacc cgcgcggatc tggccgaggc
    26041 agtcgtgaac aaggtcgggc tgccgcggaa cgaatcgcag gagctggtcg aggccgttct
    26101 gcacgaaatc gcgtccagcc tggcccgggg cgacaccgtc aagctctcct cgttcggctc
    26161 attcggcatt cgccagaagg gccagcgggt tggccgcaac ccgaagacgg ggcaggaagt
    26221 gcccatcacg ccgcgtaaag tcctggtgtt ccggccgagc aacatcatga aggaccgaat
    26281 caacgcgggt caggtccgcc gacgcaaggc ggccgcggag tagtctggac acgacgccgt
    26341 tttccatcgc gccccctgct ccgtgatcga ggttccatgt ctgtgaccaa gtcagccgaa
    26401 gccttcagga cgatcagcga agttgcagat gagttggagg tgcccaagca cgtacttcgg
    26461 ttctgggagc agaagttccc gcagatccgg cccatgaagc gcggcggcgg gcggcggtat
    26521 tatcgtccgg aggacctcga tctcctgcgc ggcatccggc acctgctcca cctcgagggc
    26581 tataccatcc gcggcgtgca gaagatcctg cgtacgcagg gtgtcgacga ggtcaagcgg
    26641 tgctggatgg agccctccaa tccgatcgcg gatgcaggcg aaggcgccgc aaagaccaag
    26701 tccaaagcga aatcgaagtc gaaggcagct cctggccgca agcaggggca ggcgagtgca
    26761 aagaagtcca gcgcctctca tctaccagag gacgtgttgc gcagcttgca gaccgctctg
    26821 tcggagctcg aagcctgccg ccgcattctg acggcgcccg tggctgacca aacctcctgg
    26881 tctcaaagcg cagaacaatg agcggcgccc cggcgggggt cgagctctgg ttcctcgacg
    26941 tggcgcgcgc cggatctgcg ctggctgcgg tcgagagatc gaccccgcgt ctgtccgccg
    27001 atgagctgga gtggacggcg gagcccggcc gcaacggcga gacccggcgc cactacttcc
    27061 gcatctgttt gcgtctcctg ctcgagcgct tcgtcggacc caggctgcgc caggttccat
    27121 tccgaactag cccgcagggc aagccacagc tgccgtggga cgcgggcgtc atgttcagcc
    27181 tctctcattc cggcccatat ggcctcatcg ccatcgccga tcgagaggtt ggtgtcgaca
    27241 tcgagcagga ccgacccgta aacttcccat tggatcgaca ggatgccatg ctcgctgcgg
    27301 caggcgcgct ctcttcagcc cccacggact cgccgctaac gattctgcaa gcctgggtgc
    27361 ggctcgaagc ctggagcaag gcgcgcgcca ccggcatcgg cgtgctgctc catgacctcg
    27421 gcatcaaggg tcctgagtgg gcggcgcgcg gtccggcaag ggacttcggg ggcatcgcag
    27481 cgagcttggc acgacaggaa ggcttcgtgc tgtacgacat cgcgctgccg cggccgcttc
    27541 atgccgctct ggccacgaag gccggtctcg cacttccgcc ccttcgcatc gtgcccgccg
    27601 acctgtcgag cctcgaggcg ctcggtgctt cacaacccga gccaagctcc ccatctgatc
    27661 caaacggttg acctctaacc gcgcccgggg caaaaggatg gctcggcgga gcgtagcgca
    27721 gcctggttag cgcaccagtc tgggggactg ggggtcggga gttcaaatct ccccgctccg
    27781 accatcaaat caggtgttcc gctggtcgag ttcagcggct tgagggcgct ttggcccaat
    27841 ttgggcgcat cggccttcgc tgcccattgc gtctggttca taggttcgat cgcgagaact
    27901 cgcgggcgcc tcgccaggtc gtcatgattc cttccttgct ggcctaccat tccggctttg
    27961 ctttccggca atacgacggt gtgcttctag gggcctttca ggattaggtg aaagtgcctc
    28021 tttccgttca agctacccag atgttagtac attcgcgcga tggcgccgcc cctctgcgcg
    28081 ccgcggattc gtgtcgaaca gaaaagagac attgccctgt agcttattgc tgcaatcgac
    28141 aatgccgagg tgagcggcag tcggggacct tcgaaggttt ttctcccgac cggaatttct
    28201 ggaatgattt ggctattgct cgacggcagt tttcgtgtgc tcggcattcg cgaacagcta
    28261 ttattatctt tccgtcgcgc tttgcccagc tttcagcaaa ccagggttgg tctgtgatca
    28321 ggactttctt tgattgagcg gttttctacg tggaatcggc ggcagcgccc cgcaacgaag
    28381 gctggtcgtc gtggtgtggc gagcgacttc gtcagcaatg aggtcgaaaa tcgtgcgctc
    28441 ttctgcagga tagaccatct gccagccata gatcgaacct ccatcgcacg agaagctgac
    28501 gtgctcgtac acgactctgt cgccacggct gccagagagg acgaaccaaa gcttgccctt
    28561 cggggtatga tcaagtctta cgccggcgta gcgggtcttg agcagagagc tgcgatagct
    28621 gccgagcgtc atgcccgtaa cattccgagc ggcaaagatg cgcagctcgg cccggtcgtc
    28681 ctccgaggtc agcacagcgc tattgtcatc cgcgaaattc gagaccgaga atacgtcggc
    28741 tggaaaaccg acggcgaagc gaaagcgatc gttgcggtag acggaccagt ttgctcctct
    28801 gccggggacc tccgttgcat caggcaccgc aaccgctgtt ccggcacgcc ccattggagt
    28861 gctgctggac aatccaggtc gttgtgccca agtgagcacg agaagggcga aactggccga
    28921 caggacgccc gctgccgttg cggtcatgca aaattgccga agccgtcgcc tctctgaggt
    28981 ctgctggcgc accaaggccc ggtcgtattc cgcgcgtgcg accgggtcga caagggtttt
    29041 ataggccctc gtcacctctt tgaagaattg ctcggcggcg gcgctgtggt tgacgtcggg
    29101 atggagacat cgagcgagtt tccggaacgc agccttcacc tggtgttggt cggccccttc
    29161 aggcagcccg agcatctggt aaagcgtact cacccacatt ccccgtgatc agaggattga
    29221 cggaggacga gcgcttggac cgtgcgcaaa tgacagcaag gttgcgcgct cactcacaca
    29281 caaccaaagc ttgtgcgaca ggtttcttca ctttatcgtc tgtggagaag cgtgtttgct
    29341 atcgcattgc tgccaaaatt gttcgggcac cccggtcggg tgcacttacc ctgcaatcag
    29401 ttcgggggat cattcactgc ctggcacgac aggtccgcat gatgagactg taatggagct
    29461 gatcgggcac tttgaagggg gtgcttgtag tcgcttaccc gctgctcaac agctcggatg
    29521 catatgattc tgcgtcttcg cctgtactct gagattgttc aaagcgcgaa caagcgcgtc
    29581 ggatccgaac ggttttggca gaaaaggcgc ttctcgcaag cccgaggcga gatgggtccg
    29641 gtcgtatccc gagatcagca ggtacggctt ccctcttgcc tgcagagcgg caacaaccgg
    29701 ctccacgcat gatccgcgca ggttcgcgtc cagaaccgcg aggtcgcagt ctcgacactc
    29761 gagtacagca agcgctttga gacacgagcc cgcgatgccg gcgatcgcaa atcccgcctt
    29821 ctgcaaggcg ttttccatct ctagcgcgag gaccggctca tcctcgacaa taagaacgct
    29881 gaccattatg gctcaccgac gctgccatcc ccactggtgt ccaggttcga gggcggatca
    29941 aggaccaact gcgcaggcgc gctcatccgc cagacgaggc cggccgggtc gtgctccagg
    30001 ctgaccgccg cgtcgagcgc gtgctcgacc atgcgcacga tgaccgtgtg cccgaaaccc
    30061 cgccgctcgg ggggcgccac gcgtggtccg ttacgttcat gccagcacat tacaaactct
    30121 ggcgcctcat cactgccggc caaattccag gttacttcca cgattccttg cgcatccgac
    30181 aacgcgccat gcttgccggc attggtggcc aactcgtgca aggccatacc gatcgtctgg
    30241 gcggcagctg ccgagagcct gagagtgggt ccatcgagca gaatacgggt gccgacaagt
    30301 ccgacgaaat gggcgagttg tgaacgcacc agctcacccg cgctcacgcc ccgccagttg
    30361 cttctgacca ggagatcctg gcacgcggcc agacaggaca agcgggcggc gaagcgctca
    30421 gcaaacacct tggggtcatt ctcaccggcc gtttgacggg cgacggcctg caccaccgcg
    30481 agcaggttct tggcgcgatg gttaacttcg cgcaataaaa accggttctg ctctgcagcc
    30541 cttttcggtc ggtgatatcc tcgaccaccg aaataaaata atcgaccgct ccgtcgtctt
    30601 tgcgcacgca gctgaccgtc agattgcacc agacgaccga gccatctttg cggcaatagc
    30661 gcttctccat ggcgtattgg tcgatttccc cggacaggac acgccgaagt agcgccaaat
    30721 tcggctcgag gtcatctgga tgagtgatgc cctgaaacgt cctcgtggtc agctcctcag
    30781 cagaccagcc gacgatttga caaagccgat cgttgacccg cagcatcgtc ccgtcagggg
    30841 cgacgtgagc gatcccgacg gcagcattct cgaacgtcgc gcggaaccgt cgctcgctct
    30901 cgcgaagtgc ctcgtcatgt ctgaagcgct ctataaagtc ggcagcttgg cgcgcataaa
    30961 gatcgagacg ccgcaaatcc tgctcgtccg gacggtgcac cgaccgaaat tgcgtcgaga
    31021 gcacaccaag cggcgcacca tcccggccaa tgagcggcgc cgccaaaatc gccctatatc
    31081 cggcagcacg cgccacggag cgaaatggcg cgtaatgcgg atcaacctcg acatcttcga
    31141 tgacgattcg ctgagccgag ccgagcgacc tgccacatgc cgaatgacta tgcggtgaaa
    31201 ttctacaaag gagctcgccg atccccggcg caaatccgtt ctgggcggcg agcaccggct
    31261 ctccgctgtc attcaggagc tgaatgaggc ccatatcagc gccggcaagc tggagtgcga
    31321 ccctgagaat ctcctccagg ccctcgcgca tactttcaat ccgccagagg cgcgagctag
    31381 attcattaag ccaatccagc gctcttgccc cactcttcag gcgggcctcc cgcaagctga
    31441 tcgccgcggc agactcgcga tgcaatttga caatctttaa gtgcagcgcg atgcgcgcca
    31501 gcagctcgtg gtcgctgaac ggccagcgca gctcatcgtc cgcgacggtc cggatctccg
    31561 ctgtcctcgg atccgagcaa cccttggaaa gaatgatgat cggaactgcg cccagcgttg
    31621 gatcagcacg catctcgccc agaactctcg agccatcgcg ttctgcccag tcggcgtccg
    31681 ctattatgac gtcgggtgcg ctggcgcggg cggcttccaa caccgattgg gggtcggcaa
    31741 tggtattgat cgtgaattgc gggctaagaa gctgtgcgac gtgggaaagc tcggcgctcg
    31801 tcccggcaag cagcaggcgt gcgcaggccg tggtgtcaga ctggccttcc gcccatggga
    31861 aagcggcgtg tgctggcgac cgatcacaga ccccctcggc ttcgcgatcg ataggcacat
    31921 tcgtccgctg catacgcaca cctgggtcca gtccggcagg atgctgatga tagactataa
    31981 ggagccggtg tgaagtgcat atccaagaag gagcggttgc gccggaacca gactggacgt
    32041 tcgatggcct ctgccaactt gcttggcacc cgtattcgcg aatggccgga aatcaatgcc
    32101 ttagcaaatc tatcggcccg acgatccgtg cgtctctcat gtaggctacg cgaccggtgg
    32161 cgggatgtca gccacggcga gcgcgatcgc tccattgtga tagtgcccga tgagggcgcc
    32221 catctccacc acgccccggt cgccaagcag attgcgggcc tgggccagcg tcgcatcgct
    32281 aacggtgttt cgctcgagca tttcagtcgt gaaagcgtaa gcc
//
LOCUS       DASVDU010000019        25151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_733398, whole genome shotgun sequence.
ACCESSION   DASVDU010000019 DASVDU010000000
VERSION     DASVDU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 25151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733398"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VF226_01440"
     CDS             complement(<1..1091)
                     /locus_tag="VF226_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7072680.1"
                     /translation="MSEKVYAVPPEWASRAWVDAVKYQEMYQRSISDPDGFWGEMGKR
                     LDWIKPYTRVKNTSFDPNNVSIKWYEDGTLNVCYNCIDRHLEKRGDQVAIIWEGDDPT
                     VDEKITYRQLHERVCKFANVLKANGVQKGDRVTIYLPMIPEAAYAMLACARIGAMHSV
                     VFGGFSPDSLAGRIEDADSKFIITADEGLRGGRKVPLKKNTDEALKKTKGGEKVLVVR
                     RTGGDVPWTPGRDIWLHEELVKVSSDCPPVEMGAEDPLFLLYTSGSTGKPKGVLHTSG
                     GYLVHVSLTHQHVFDYHDGDIYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPNYP
                     TPSRFWHVVDKWGVNIFYTAPTAIRALMGA"
     gene            complement(1202..1624)
                     /locus_tag="VF226_01445"
     CDS             complement(1202..1624)
                     /locus_tag="VF226_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX7072681.1"
                     /translation="MSFGGVNYLAVLLAAVASFLFGGAYYSAFSRRSLAESADEATEP
                     RRRQLHADAVPFIVAFVALLIMAFVLAGAISSLGAGQVTVRNGIISALLAWVGFVMTT
                     LMINHMVQRVSRIATLVDGGHWLGVLIIQGAVIGWFGL"
     gene            complement(1621..2322)
                     /locus_tag="VF226_01450"
     CDS             complement(1621..2322)
                     /locus_tag="VF226_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7072682.1"
                     /translation="MKGIDAVSFIHANTELLAPPLTPEIVLHLAAESLPIWKSTEEEL
                     GEMNVPPPYWAFAWAGGQALARYVLDNPESVAGKSVVDLGAGSGLVAIAAARAGAASV
                     LAIDIDALAAAAIELNAAANGVRIVASCRNVFDEGLPAADVLLVGDLFYERELANSVL
                     ELIEAVAREGTDVLVGDPRRSYFPVERFDQVASYEVPVTRELEDAEIKRTAVWRLSMP
                     PARSSFLAAESMEAA"
     gene            complement(2381..2836)
                     /locus_tag="VF226_01455"
     CDS             complement(2381..2836)
                     /locus_tag="VF226_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX7072683.1"
                     /translation="MRYWLFKSEPDAFSWDMLKAKGEAGEPWDGVRNYLARNNMRAMQ
                     VGDLGFFYHSNQGKEIVGVVEVIAPAHPDPTDTTGTWECVDVKAVADVPRPVKLNDVK
                     ATPELANMALVTSSRLSVQPVTDEEWEVVCRMGGLDPKKLRSKPRRKVA"
     gene            complement(2985..3986)
                     /locus_tag="VF226_01460"
     CDS             complement(2985..3986)
                     /locus_tag="VF226_01460"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7072684.1"
                     /translation="MRERARRVGVIGAGAWGTALALVAARAGHQVILWARHAETAAAI
                     NTAHENKARLPGVHLPDAIVATSQPAALGDVDLLLIASPAQAMREVMAQLGPHLAGGI
                     PAAIAAKGIEQTTSLFMTDVLSEVSPGLEPLVLSGPSFAGDVARELPTAVTLAARSLE
                     IARPVAEALSLPTFRPYISDDLVGVQVGGAVKNVLAIACGIVTGRSLGESARAALIAR
                     AFAELTRFGQALGARRETLSGLSGLGDLVLTCSSPQSRNFQLGFALGQGKSLADATAG
                     TRGISEGVYTAAAVVQIAAERCIEVPVSMAVHEILAGRLTISQAIDGLMQRPLKAED"
     gene            complement(3983..5089)
                     /gene="tsaD"
                     /locus_tag="VF226_01465"
     CDS             complement(3983..5089)
                     /gene="tsaD"
                     /locus_tag="VF226_01465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7072685.1"
                     /translation="MSQQPANGRAGTLVLGIESSCDETAAAVVLREGPAQGRLLSNVV
                     RSQWEQHRQFEGVVPEIAARAHVECLDDIIREALSGAGVALCDVDGIAVTAGPGLIGG
                     LLVGLTTAKALALATEKPLIAVNHLEAHALTVGLTEGLPPPYLLLLVSGGHTQLLLVR
                     DVGQYDRLGTTIDDALGEAFDKTAKLLGLGFPGGPAVERMAEEGDPRRFAFPRPMIGR
                     DEPHFSFAGLKTAVRRQAMQLVPLTEEDIRDLCASFEAAVADVVVDRCARAMRLTEDQ
                     LGPEKHLVIAGGVAANRRLREALSRLAEASGFRLHAPPIALCGDNAAMVAWAGAERLV
                     RGHVDSLDVPARARWPLDQIAEPVLGRGRLGAKA"
     gene            5202..6131
                     /gene="hemC"
                     /locus_tag="VF226_01470"
     CDS             5202..6131
                     /gene="hemC"
                     /locus_tag="VF226_01470"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7072686.1"
                     /translation="MQADRIRIGSRGSPLALAQANEVKQRLVSAHGLDASAVEIRTFK
                     TTGDRIQDRPLAEAGGKGLFTKEIEEALLAGEIDLAVHSMKDMPTELPGGLTIASMLP
                     REDVRDAFISLRYASLAELPSGAVVGSSSLRRQAQVRRLRPDLNVIEFRGNVGTRLRK
                     LEQGVAEATFLACAGLKRLGQADRITSVMPSEEMLPAVAQGAIGIEIRSDDAKTARLV
                     AALNHGPTALCVETERAFLARLEGSCRTPIAGLAELHDGVIHFRGLILSPDGTRFHAT
                     QRQGSPADARAMGDDAGRELRAAAGPDFFQWSD"
     gene            6131..6862
                     /locus_tag="VF226_01475"
     CDS             6131..6862
                     /locus_tag="VF226_01475"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7072687.1"
                     /translation="MRLLVTRPEPDATALRAHLIAQGHEVLTEPLITIKFDNADPIEL
                     DEVQALIATSRNGLRALAASPALEQARSLPLFAVGPGTAATAKALGFQHVIEGPGTGR
                     QLVSFIVEHAEVNGGPLLHLAGDVLAYDFASELSRLGYHVLRPVVYETETATRLSGST
                     AARIANGQIEGVLLLSPRTAATFAALIKAHGLTEAARRLIYFCISQAAAAQLEEIAPV
                     KVEIASEPNLQEVLALTARATPQSS"
     gene            6971..8830
                     /locus_tag="VF226_01480"
     CDS             6971..8830
                     /locus_tag="VF226_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072688.1"
                     /translation="MTNRNDSSKTTGKPASEGSGPKKPSALLDLKATEVTSKDVKPPA
                     PSTSTTASAAAGSGGSAKEAEPKGSPAPASSPAAANVENSPGATSGAPKPAASQTSSS
                     GTSKPGTQSAEKQTVQQPAGKQDAAKSSAQQETTPAATPKRSGSGLSIVTHLTAGIAG
                     GFLALLLADAVGPQFGLGNRVPQQVVSDLQQRLTAAEQAIRQRSAIETVSPQEFQQKL
                     AEVESRLAHLDEVDRKVGELSDNQAKIAAETTNLADRVNQSAPAEVTERIAKLEQVLS
                     TLTAAAGSDEPGRIPQLAAISGKLADLEATIPNQLTELRKSVSEEIATRLSETAEASE
                     AARAATLRMDRELAAAKADLARLSQRAEALKATDDRLEQLLRAVQEEAGSLRSTVEGL
                     QGELATQLKTVARAQDVTAAVAPVEGRVAALEQNVQAVVRSEEDRRANAERIVLALEL
                     GNLKRALDRGGSYAAELGELRRISAGKIDLAVLERYKDEGVPTLAALQKEFRPVANAV
                     IDAASVPADASPLDRLLIGAKAIVRVRRVEHSPDDTSVEAIVGRMEQALAEGKFTDVL
                     DQARLLPPKSLEPAEAWLAKVEARAAVDRALASVEDQLKASLSGQAAVEKRTN"
     gene            8830..10809
                     /locus_tag="VF226_01485"
     CDS             8830..10809
                     /locus_tag="VF226_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEX7072689.1"
                     /translation="MLRLVLFLLAVSALAFGLSWLADRPGSLIVNWQGYEIETSVFRA
                     IVILAVLLGLAIVLWSVLRQIWHSPAAIGSYFNRRRQKRGLEALSSGMIAIGAGDRAL
                     ATRYAIQARKSLPNEPLTHLLRAQAAQLSGDRATSRRIFEAMLASPDTEQLGLRGLFL
                     EAEREGEQEAARQFAERAMALNPKLSWPVEALFDIQCRKGDWAGALETLAVAKRHHNI
                     ERAVADRRRAVLLTAQAQELEETDPERAMTLALEAHNLAPDLVPAAAIAGRVLAAKGN
                     IRRAAKVLQRTWKRSPHPDIAVAYAYARIGDSPRDRLDRVKQLAALRPHSMESAIAVA
                     TAAIEAQDWPTARHVLQPLLANAPTQRTCTLMARIEAEQHGDRGRVREWLARAVNAPR
                     DPAWTADGVVSEKWAPTSPVTGALDAFQWRVPVEAVNASEATLLSQKLEELVNLHPEP
                     EAIPQGPLIDAPSAAPVADEAPETDAEAAAEAKPAAPGPQKTSAPPASALPAEPGPEA
                     QEPESAAGPGPAAPSAEAAPTAAPSPKKPAEAAQSPAPEPAPAKEAAPAKTTSGAESS
                     SASARPTEQPTPEAAAAKALSPDLDTPAPGQPAPPAHMGIKTYPQPFRTRTRPVEPRI
                     FVPPRAPDDPGPDADGEEDLSRSRVGFHLPIKGSV"
     gene            10893..10968
                     /locus_tag="VF226_01490"
     tRNA            10893..10968
                     /locus_tag="VF226_01490"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10926..10928,aa:Thr,seq:cgt)
     gene            11630..12145
                     /locus_tag="VF226_01495"
     CDS             11630..12145
                     /locus_tag="VF226_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEX7072690.1"
                     /translation="MKPSVLLSSLLALSLVAGAATQVTAQQRGPGWGMMGGYGMMHGP
                     GWGRGLGMGFGNCPMWDDGGAPTFAQGRIAFLKAELSITDSQASAWEAYANALKKNFE
                     SMQGMRQTMLAAWQAKSPVERLNAHITAMEGRLAALKDIKAPLETLYAALNDEQKKKA
                     DDLLTGMGCMM"
     gene            complement(12222..12806)
                     /locus_tag="VF226_01500"
     CDS             complement(12222..12806)
                     /locus_tag="VF226_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7072691.1"
                     /translation="MTGLTEKARAGGDLPPAEAANGLPETIRVWDPLVRVFHWSLVLL
                     FATAWLTGDEVEWLHIYVGYGIAGLLAVRIVWGFAGTRHARFSDFVYRPSEVVSYLAD
                     SVRLRAKRYIGHNPAGGAMVIALLGVLAATAGTGILMTSDAFYDAHWLEEVHEALANL
                     ALVLVGLHVAGVILASFEHGENLVSAMITGRKRR"
     gene            complement(12806..13087)
                     /locus_tag="VF226_01505"
     CDS             complement(12806..13087)
                     /locus_tag="VF226_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7072692.1"
                     /translation="MRKALMTSALVLATATAAGLAVASDDDVRVNVPKEQWLSIAQIA
                     EKLTAQGYDIREIEIDDGAYEVSATKDGKRVKAYLHPATGEILRQKTRN"
     gene            13217..13564
                     /locus_tag="VF226_01510"
     CDS             13217..13564
                     /locus_tag="VF226_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7072693.1"
                     /translation="MRTELDRRTLLLALAALGTGSPALARGDDDRSHRKRSSRHDHEQ
                     ARRALEEGRARPLADILAEVRPRLGGEVIDVEFEREDGRYVYEFKVITPAGQLREIEV
                     DALTAEILKDEAD"
     gene            13564..14229
                     /locus_tag="VF226_01515"
     CDS             13564..14229
                     /locus_tag="VF226_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7072694.1"
                     /translation="MRILVVEDEPRIAADVRAALEAAGYLVDIARDGDEAWFRGDTED
                     YDLVVLDLGLPKIDGLAVLKRWRAAGRAMPVLVLTARGSWSERVEGIDAGADDYLPKP
                     FRMEELVARVRAVIRRSAGHADSVIEVGPLTLDIRQMRLTKDGVPVALSPLEYRLVAY
                     LMHHKGRVVPGPELLEHLYGDDDAHEANALEAVVARLRKKLGPDAIETRRGFGYLIAA
                     EDA"
     gene            14226..15578
                     /locus_tag="VF226_01520"
     CDS             14226..15578
                     /locus_tag="VF226_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7072695.1"
                     /translation="MNGGSLRLRLFLGGALSIIAALALAALGLVLLFEKHVERRIESE
                     LGVYLDQIIANLDRDGDGALVVADVLADPRFDQPLSGLYWQVRAKDAVLRSRSLWDGD
                     LPLPSDKLADGVVHRHRIIGPAGAELLALERSVRLPARLGNASVRAAVALDTREIAAA
                     TRAFAADLLPYLGVLAAVLIAAAYAQVTFGLSPLATIRKRLAGIREGNATRLGKAFPK
                     EVQPLAAEVDALLEAREQEAERARSRAADLAHGLKTPLQVLAGDVARLRQKGEADIAA
                     EIEQVATAMRRHVERELARARMKSGVSTARANVREVIDRVLAVVMRTPDGARLAWTVE
                     CPPQLLARIDADDLAEAIGNLVDNAARHARRAVRVEARAEDRRVKLLISDDGPGIPPE
                     RMSEVLARGGRLDESGTGAGLGLAIVQEIADAWAGRLELRPNGPGLQAELALDLVRGP
                     "
     gene            15620..15847
                     /locus_tag="VF226_01525"
     CDS             15620..15847
                     /locus_tag="VF226_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072696.1"
                     /translation="MRFGLLPVRTRVGGLLRASGEKPEWLGSMPDAGHLAASPGARAG
                     RLIVMAGGEVESQSFGYMGHKARASPSWATR"
     gene            complement(15997..16992)
                     /locus_tag="VF226_01530"
     CDS             complement(15997..16992)
                     /locus_tag="VF226_01530"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7072697.1"
                     /translation="MISSRTISIALMCAGLLLPAAAQAGPKLLFDPADGKVLYAEDID
                     DQWYPASLTKMMTAYVVFEALKAGQITLETKLVTSEAAHAQPPSKIGLPVGGEMSVQL
                     GLESLVVKSANDVAIMLAEAVGGSEPAFVERMNATALRLGMTNTHFVNANGLPAAGQA
                     TTARDLAKLAMAVTRDFPDYRYLWGLAEFRIGKRRIATHNNLLKTYDGADGLKTGFIC
                     DSGYNIVASATRDGRQLMAVVLGEPSSQERAQRASNLLDHGFATYDWKVLFGTTTLDT
                     LPVSENPRPLTSVRDTVTSWSCGRRPARVVKKGGRKPVAKARASTAAAPSAARTQ"
     gene            complement(17380..18585)
                     /locus_tag="VF226_01535"
     CDS             complement(17380..18585)
                     /locus_tag="VF226_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7072698.1"
                     /translation="MTPTIATSAPHRPLLPILFALSGAHLLNDLIQSMIPALYPLFKQ
                     TYNLDFVQIGLITVAFQVTSSLLQPLLGFVTDHRPWPYAMVAGMAATLSGLLGLAFAT
                     SYGTLLVSAAMVGLGSAVFHPEATRMARHAAAGEQGFAQGIFQIGGHAGYAIGPLLAA
                     MIVVPHGQASLAWISVVALTAMVLMAWTGWRYAAMRRSQRAAGKSSTTGAAARSLPVG
                     HVLMAMAILIVLLLSKNGYTAAFTSYYTFYLIERFGVSVPFSQIMLFLYLFVGAIGVV
                     VGGMIGDRIGRYQVILVSILGTLPFAILLPYANLFWTGVLSVVINLIMASAFASILIY
                     AIDLVPHRVGLVGGLFYGLAFGLGGLAAAAIGLLADQIGIIAVFKICAWLPALGLLAF
                     FLPRASRGA"
     gene            complement(18768..18929)
                     /locus_tag="VF226_01540"
     CDS             complement(18768..18929)
                     /locus_tag="VF226_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072699.1"
                     /translation="MTNADLPGLPLPLSGETRLYTIIGDTIDQVGSPPVAAPFSAESA
                     CSKGSGCHL"
     gene            complement(18980..20110)
                     /locus_tag="VF226_01545"
     CDS             complement(18980..20110)
                     /locus_tag="VF226_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX7072700.1"
                     /translation="MTLDIRNVTPFVYRCPLDEPVTTSFGTMRDRPMVLVRVEDREGA
                     VGWGEIWCNFPAVGAEHRARLVDSVLAPLSCEKTFASAQEAFEHLTRRTAVLAIQSGE
                     AGPIAQAIAGLDIALWDLEARRAGQPLWKFLNPGAAPNIQVYASGLNPTNPEGLALAR
                     QKEGHIAFKLKIGFGIERDVRNLQALRTSLGPAALLMVDANQAWDVRTALDVLPRLEP
                     FQLQWLEEPLRADEPWSEWAKLRERTSVPLAAGENIMGLEAFDLALKSGVLSVVQPDI
                     AKWGGFSGCFPVAKRIIAAGRTYCPHYLGGGVGLLASAHLLAAAGGDGRLEIDSNPNP
                     LRTLLSGPVQTICDGKVTLPDRPGLGIEPDAVHLKEYAVSWR"
     gene            complement(20107..20844)
                     /locus_tag="VF226_01550"
     CDS             complement(20107..20844)
                     /locus_tag="VF226_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7072701.1"
                     /translation="MSYSSHHIFGATPIPRYVQLAELFRERVNRGFWRPGSILPSIEQ
                     LMAEFGVSRVTVRQAIALLAEEGLLSPQRGRGTFVMSKAGPRRKLRVETTLDDLVTMY
                     RGDRPQHTTLDEGIAEPALDESDGAKAPKYFYMRRVHERDGERYCVISIYIEYRIFRR
                     APSRFRRELVIPILTSLPGLQISSARQTLTISSADVTTAQLLGISINAPVAEVRRVMC
                     GTDGTVIYLAEVTYRGDYIHLEMDLKP"
     gene            20929..21678
                     /locus_tag="VF226_01555"
     CDS             20929..21678
                     /locus_tag="VF226_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072702.1"
                     /translation="MRRTALILMPWIAILAVWYAIHHSGLVNPALVPTPHEVGRRFIE
                     LLRGRLPLDILMSTQRVFLGVLSGITIAVPVGFVLGWYRNIRAFVDPLINFFRALPPI
                     ALIPLVIVYFGVDELAKTVILFYASFFSGVIVMYEGISQISPIYVRVARTLGANDGEI
                     FRKVVIPLAVPHMLTALRVALGVAWATLVASELIAAQQGLGAMIQDAGSFFQLDIIYV
                     GIICIGFIALFMDLALRAIARKFVSWQERIV"
     gene            21675..22481
                     /locus_tag="VF226_01560"
     CDS             21675..22481
                     /locus_tag="VF226_01560"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072703.1"
                     /translation="MNAGRVRIAFDRVSVRFPTQDNGEILVVDEVSYDIHDREFVSII
                     GPSGCGKTTMMNIVAGFMQPSSGSVTLDGKPITGPGPDRGVIFQEYGVFPWLSVKENI
                     AFGLNLRANRTSAAEREEICERYMRLMGLSEFANAWPRMLSGGMRQRLALARAYAVKP
                     QFLLMDEPFGALDAQTRSAMQDLLLEVLAAEGKTVMLITHSVEEAVYLSNRIIVMTAR
                     PTRIREIIEVPFAYPRTDDLHESKEFGELRAHVRNLVMQEYAQQALQHQT"
     gene            22515..23510
                     /locus_tag="VF226_01565"
     CDS             22515..23510
                     /locus_tag="VF226_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072704.1"
                     /translation="MNRRTLIKSSLAVAGAAAIGAPAIAQSKTPIRVGYLHTLAVDGQ
                     MWLADHLGAYQKHGLEPEFKLFQTGLELFQAMIGGSLDVLSTGAVISNFPARGQGKMF
                     LTNGVEFATAQLWVREDQGIKSIADLKGKRVATTAGTTAHVFLDTALKANGLQSSDIE
                     ILNQRMPDAVTSFISGSVPAVALWVPFNITVRDKVPQAKKLIDASAFYPDAAIMSGWA
                     TTADFHAKNRDTLIRLIQAWAEGNEYLTGKTDEALEILQKKHYPQVPLADFKEQYAAQ
                     KSFPVAEWRKLYSDGTVTKWLQQVTDFFAATGNIPNPVPASEYFDPTLFMEATKA"
     gene            23514..25127
                     /locus_tag="VF226_01570"
     CDS             23514..25127
                     /locus_tag="VF226_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7072705.1"
                     /translation="MPNQTYDYIIIGAGSAGCVLANRLSADPSCKVLLLESGGWDRNF
                     WIKLPVGYFRTIYDERFSRLFPTEPSEGTAGRGIVWPRGRIVGGSSSINGLIFIRGQR
                     EGFDDWESLGASGWGYEDVLPYFRKLECFNGEPSQYRGAHGELRVSILRNDHPACRAW
                     IDAAQQFGLPYNPDFNGETTYGVGSYQLSIGPRWRESAAVAFLRPALSRPNLTVLTRA
                     HATRIAFEGTRAVGVYWVQRGREELARAEQEVILSAGALQSPQLLQLSGVGPADLLRQ
                     HGVPVIADLPGVGENLQDHYQARTIVRLTSPISLNDDVRNPLRLAHMGWRWLVRGEGP
                     LTIGAGQVGGAACTQYAVGGRPDIQFNVMPLSVDKPGDPLHRYSGFTASVWQCHPVSR
                     GRLTIRSSDPFAPPRIEPRYLDAELDRKVMVEGVKILREIYHQPAFRKLWTDEVRPGL
                     AAVADEAILAFIREQGGTVFHACGTCRIGNDDGAVVDSFLRVRGVDRLRVVDASVMPL
                     ITSANTNAASLMIGEKGAALIKGEARAPS"
BASE COUNT         4561 a         8246 c         7986 g         4358 t
ORIGIN      
        1 gcacccatca aggcgcggat cgccgttggc gccgtgtaga aaatgttcac cccccactta
       61 tccacgacgt gccagaaccg cgacggcgtc ggatagttgg gcaccccctc gaacatcagc
      121 gtcgtggcac cgttcgccag aggcccgtag acgatgtagc tgtggcccgt cacccagccg
      181 acatcggccg tgcaccagta gatgtctcca tcatggtagt cgaagacgtg ttgatgggtg
      241 agcgacacgt ggacgagata gccacccgac gtgtgcagca cgcccttcgg cttgcccgtc
      301 gagcccgacg tgtagaggag gaacagcggg tcctcggcgc ccatctcgac cggcggacag
      361 tcggaactga ccttcaccag ctcctcgtgc agccagatgt cccgccccgg cgtccagggc
      421 acgtcgccgc cggtgcggcg cacgaccagc accttctcgc cgcccttggt cttcttcaag
      481 gcctcgtcgg tgttcttctt gagcggcacc ttgcgcccgc cgcgcaggcc ctcgtcggca
      541 gtgatgatga attttgaatc ggcatcctcg atgcgaccgg cgaggctgtc gggcgagaag
      601 cccccgaaca cgaccgagtg catcgcaccg atgcgggcgc aggccagcat ggcataggct
      661 gcctcgggga tcatggggag ataaatcgtc acccggtcgc ccttctggac gccgttggcc
      721 ttcagcacgt tggcgaactt gcacacccgc tcgtggagct ggcgataggt gatcttctcg
      781 tcgacggtcg gatcatcgcc ctcccagatg atcgccacct gatcaccgcg cttctcgaga
      841 tgccggtcga tgcagttgta gcagacattc agagttccat cctcgtacca cttgatggag
      901 acgttgtttg gatcgaagga ggtattcttg accctggtgt agggcttgat ccaatcgagg
      961 cgcttgccca tttcacccca gaacccatcg gggtcggaga tcgagcgctg gtacatctcc
     1021 tggtacttta ccgcatcgac ccacgcccgg ctcgcccact ctggcggaac ggcatagact
     1081 ttctccgaca tcgagaaacc tcccaagatg cttgagtaaa atttggcgca ttatgtcggc
     1141 cgtattccgt gccgacaatg gcgccgttgg tcttaaatgt cacgtacgcg cccatggcag
     1201 cctagaggcc gaaccagccg atgacggcgc cctggatgat cagtacgccc agccaatggc
     1261 cgccgtcgac cagtgttgcg atacggctga cgcgctgcac catgtgattg atcatcaatg
     1321 tcgtcatcac gaagccgacc catgcgagga gcgcggaaat gatcccgttc cgcaccgtga
     1381 cctgaccggc cccaaggctg cttatggctc ccgccagcac gaaagccatg atcagcaggg
     1441 cgacgaacgc gacgatgaac ggaacggcat cggcatggag ctgccgacga cggggctcgg
     1501 ttgcctcgtc ggccgattca gccagggagc ggcgcgaaaa agcggaataa taggcccccc
     1561 cgaacaagaa gctggcaact gctgcgagca gcacggccag ataattcacc ccaccgaagc
     1621 tcatgccgcc tccatgctct ctgcggctag gaagctggac ctggccggcg gcatgctgag
     1681 ccgccagact gccgtccgct ttatctctgc gtcctcgagc tcgcgcgtga ccggcacttc
     1741 atagctcgcg acctggtcga atcgctccac gggaaagtag ctgcgtcgcg gatcgccgac
     1801 caggacgtcc gtcccttcgc gcgcgacagc ttcgatgagt tccagaacac tgtttgcgag
     1861 ctcgcgctcg tagaagaggt cgccgaccag aagcacgtcg gccgccggca gcccctcatc
     1921 gaagacattc cggcaggagg cgacgatccg gacgccgttc gcggcggcat tgagctcgat
     1981 ggccgctgcc gccagcgcgt cgatgtcgat cgccagcacc gatgcggctc ccgcccgggc
     2041 cgccgcaatg gccaccagtc cgctgccggc gccgaggtcg acgaccgatt tgcccgccac
     2101 actttcaggg ttgtccagca cgtaccgcgc cagcgcctga ccgccggccc aggcgaaagc
     2161 ccagtagggc ggcggtacgt tcatttcgcc cagctcctcc tccgtactct tccagatggg
     2221 gagcgattct gcggcaaggt ggaggacgat ctccggagtc agtgggggcg cgagcagctc
     2281 cgtgttcgca tgaatgaagc tgacagcgtc gatgcccttc atacccgcct gcatacctgg
     2341 accgcgcgac cacaggacat ggccggcaag cagctgccgg tcacgcgacc tttctccgcg
     2401 gcttgctcct gagcttcttg ggatcaagtc cgcccatccg acaaacgacc tcccattcct
     2461 catcggtaac cggctggacc gaaagccgcg aggacgtcac gagcgccatg ttggcaagct
     2521 ccggcgtcgc cttcacgtcg ttgagcttta cgggccgcgg gacatccgcg accgccttga
     2581 cgtcgacgca ctcccatgtg ccggtggtgt ccgtggggtc ggggtgcgcc ggcgcgatca
     2641 cctccacgac gccgacgatc tccttgccct ggttggagtg atagaagaag ccgaggtcgc
     2701 cgacctgcat cgcgcgcatg ttgttgcggg caaggtagtt gcggacgccg tcccacggct
     2761 cgccggcttc gcccttcgcc ttcagcatgt cccacgagaa ggcatccggc tcggatttga
     2821 aaagccaata gcggatgcgc ggatcggctg agggcttggt ggcgggcttc ttcggcatat
     2881 caagctgacg ctgaatgatg gcgcactcgc gacaaaggct agctgaataa acccagctgg
     2941 caagtgctgg aggcgcggcc gaatgggccg cgataagtta acccttagtc ctcggccttc
     3001 agcggccgct gcatgaggcc gtcgatcgcc tgagagatgg tcaggcgacc ggcaaggatc
     3061 tcgtgcaccg ccattgatac aggcacctcg atgcaccgct ccgctgcaat ctggaccacg
     3121 gcagcggccg tatacacgcc ttcgctgatg ccgcgggtgc cggccgtcgc atccgccagg
     3181 ctcttgccct gccccagcgc aaaaccgagt tgaaaatttc gcgattgcgg gctcgagcag
     3241 gtgagaacga gatctccaag ccccgatagg ccgctcaggg tttcccggcg tgcccccaga
     3301 gcttgcccaa aacgcgtcaa ttccgcgaag gcgcgggcaa tcagagcagc gcgtgcactt
     3361 tcccccaggc tccggccggt cacgatgccg caggcgatgg cgagcacgtt cttcaccgcg
     3421 ccgccgacct ggacccccac gaggtcgtcg gagatgtagg gccggaatgt cggcaggctc
     3481 agcgcctcgg ccacagggcg ggcaatctcg aggctgcgcg cggcgagcgt gacggcagtc
     3541 ggcaactcgc gggcgacgtc gccggcaaag ctggggccgg aaagcaccaa cggttcgagg
     3601 cccggggaca cctccgagag cacatcggtc atgaagaggg aggtggtctg ctcaatcccc
     3661 ttcgcggcga tggcagccgg tatgccgccg gcgagatgcg gcccgagctg agccatgacc
     3721 tcccgcattg cctgtgccgg gctggcaatc agcagcaggt cgacatcccc gagagcggcg
     3781 ggttgtgaag tggccacgat cgcatctggc agatgcacgc ccggcagccg cgccttgttt
     3841 tcgtgcgccg tgttgatggc tgcagcggtc tctgcgtgcc gtgcccaaag gatgacctga
     3901 tgcccggcgc gtgcggcgac gagcgcgagg gccgtgcccc acgcaccggc accgatcaca
     3961 cccactcgcc gcgcacgctc cctcatgcct tcgctccgag gcggcctctc ccgagcacgg
     4021 gctcggcgat ctggtccagc ggccagcgcg cccgtgcggg cacgtcaagg gaatcgacgt
     4081 gtccccggac gagacgctca gccccggccc aggcgaccat cgccgcgttg tctccgcaca
     4141 gcgcaatcgg aggagcatgc agcctgaagc cgctggcctc ggccaaacgc gacagcgctt
     4201 cgcgcagccg ccggttggcg gcgacgccgc cggcgatcac caagtgcttc tcgggcccga
     4261 gctggtcctc ggtgagccgc atggcccgcg cacagcggtc gacgacaacg tctgcgacgg
     4321 cagcctcgaa gctcgcgcac aggtcgcgga tgtcctcctc cgtcaggggg acgagctgca
     4381 tggcctgccg ccgaaccgcc gtcttcaggc cggcgaagga gaaatgcggc tcgtcccgcc
     4441 cgatcatcgg ccgggggaac gcaaagcggc gcggatcccc ctcttccgcc atgcgctcca
     4501 cggcggggcc gccgggaaag cccagtccca gcagcttggc ggtcttgtcg aaggcctcac
     4561 cgagcgcatc atcgatggtc gtgccgagcc gatcgtactg gccgacgtcg cgcaccagca
     4621 ggagctgagt gtgcccgccg gaaacgagca gcagcaggta gggcggcggc agcccttccg
     4681 tcagccccac cgtgagcgcg tgcgcttcca ggtggttcac cgcaatcagc ggcttttccg
     4741 tggccagcgc cagggccttg gccgtcgtga gcccgacgag caatcccccg atcagccccg
     4801 gcccggcagt caccgcaatg ccgtcgacat cgcagagcgc cacccccgcc ccggacaacg
     4861 cctcccggat gatgtcgtcg aggcattcga catgggcgcg cgccgcgatc tccggcacga
     4921 ctccctcgaa ctgccggtgc tgctcccact gcgagcgcac gacgttcgac aacaaccgtc
     4981 cctgcgccgg cccctcgcgt agcaccacgg cggctgcggt ctcatcgcag ctcgactcga
     5041 tgccgagcac cagagtgccg gcgcggccgt ttgcaggttg ctggctcatg cggcgtcgtg
     5101 atccatctcg cgcaatttgc gtcttcgcgc tgcggcgggc atgaatggcg gcgacaggcc
     5161 cggcagacgt aagctcgggc ctagcatccg gagattggcg cgtgcaagct gacaggatca
     5221 ggatcgggag ccgcggatcg ccgctggcgc tggctcaggc caacgaggtg aagcagcgcc
     5281 tggtttcagc gcacggcctg gacgcgtctg ccgtcgagat ccggacgttc aagaccacag
     5341 gcgatcgtat ccaggatcgg ccacttgcgg aagcgggcgg caaagggctc ttcacgaaag
     5401 agatcgagga ggccctgctc gcgggcgaga tcgatctggc cgtccattcc atgaaggaca
     5461 tgccgaccga gcttcccggt ggcctcacga tcgcgtccat gctgccgcgt gaggatgtgc
     5521 gtgatgcctt catcagtctc aggtacgcct cgcttgcaga gctgccttcg ggcgctgtcg
     5581 tcggcagctc atctctgcgc cgtcaggcac aagtgcgccg gttgcggccc gatctgaacg
     5641 tcatcgagtt ccgcggcaat gtggggacca ggctgcgcaa gctcgagcag ggcgttgcgg
     5701 aggccacgtt cctagcctgc gccggactga aacggcttgg acaggcggac cgcattacaa
     5761 gcgtgatgcc gagtgaagag atgctgccag cggtggccca gggcgcgatc ggcatcgaaa
     5821 tccgcagcga tgatgccaag acggcacggc tcgtcgccgc cctcaatcat ggtccgaccg
     5881 ccctgtgcgt ggaaacggaa cgcgccttcc ttgcacggct tgaagggagc tgtcgcacgc
     5941 ctatcgctgg gctggccgag ttgcatgacg gcgtcattca cttccgcggc ctcattctgt
     6001 ctccggacgg cacccggttc cacgccaccc agcggcaagg ctcacctgca gacgcgcgcg
     6061 ccatgggcga cgatgccggc cgcgagctgc gcgcggctgc ggggccagac ttcttccagt
     6121 ggtccgactg atgcgtctcc tcgtcacacg tcccgagccc gacgccactg cccttcgcgc
     6181 gcacctgatc gcgcaagggc acgaggtcct caccgagccg ctgattacga tcaagttcga
     6241 taatgccgat cccatcgagc tcgacgaggt ccaagcactg atcgcaacca gccgcaacgg
     6301 cctcagggcg ctcgccgcca gccctgccct ggaacaggcg cggtcgctgc cgctgttcgc
     6361 ggttggcccg gggacagcgg cgaccgcaaa ggctctcggc ttccagcacg tcatcgaggg
     6421 cccgggcacg ggccgacagc tcgtcagctt cattgtcgaa catgcagagg tgaacggcgg
     6481 cccactgctt cacctcgcag gagatgtgct ggcctacgac ttcgcaagcg agctgtcgcg
     6541 cctcggctat cacgtcttgc ggcccgttgt ttacgagacg gagacggcga cgcgcctcag
     6601 cggctcgaca gcggcacgca tcgccaacgg gcaaatcgag ggcgtgctgc tgctgtcgcc
     6661 gcgcacggcc gcaactttcg ccgcccttat caaggctcac ggcctcaccg aggccgcccg
     6721 ccgcctcatc tacttctgca tctcacaggc cgctgcagca cagcttgagg aaattgcccc
     6781 cgtcaaagta gagatcgcca gtgaacccaa cctgcaggaa gtgcttgctc tgacagcacg
     6841 cgccacgcca caatcgtcct agacactcaa cccggaaaaa gcgccagtga tcaggcacga
     6901 agccttaacc gacccgagcc atcagtgagg gcgcaaggac tcccgctggc acgagaggct
     6961 agcttctgta atgacgaata gaaacgacag ctcgaagacg accgggaagc ctgcttccga
     7021 gggctccggc ccgaagaagc catctgctct gctcgatctg aaggcgaccg aggtgacgag
     7081 caaagacgtc aagccgccag ctccgtccac ctcgacgacg gcatcagcgg ccgctggatc
     7141 tggcgggagt gccaaggagg cggagccgaa gggcagcccc gcccctgctt cgtcgccagc
     7201 cgcggcgaac gtggaaaatt ctccgggcgc cacgtccggc gcaccgaagc ctgccgcaag
     7261 ccagacaagc tcatctggaa cttcgaagcc tggaacccaa tcagcggaga agcaaacggt
     7321 tcaacagccg gctgggaagc aggatgcagc caaaagctcc gcccagcagg agaccacgcc
     7381 cgcggcaacg ccaaagcggt ctggaagcgg gctgagcatc gtgacccatc tgacggccgg
     7441 cattgccggc gggtttctgg ccttgctgct cgccgatgcc gttgggccgc agttcgggct
     7501 cggcaatcgc gtgccccagc aggtcgtcag tgatttgcag cagcgcctca cagcggccga
     7561 gcaggcgatc cgccagcgtt ccgctatcga gacggtttcg ccgcaggaat tccagcagaa
     7621 gctcgccgag gtcgaaagcc gattggcgca cctcgatgag gtcgaccgga aagtcggtga
     7681 gctttcggac aatcaggcga agatcgccgc cgaaaccacg aacctcgcgg atcgcgtcaa
     7741 ccagtccgcc ccggcggagg tgaccgagcg catcgcgaaa ctggagcagg tcctctcgac
     7801 gttgacggcg gctgccggca gcgatgagcc cggtcgcatt ccgcagctcg ctgccatcag
     7861 cggcaagctc gccgatctcg aggcgaccat tcccaaccag ctcaccgagc tacgcaagag
     7921 cgtgagcgag gagatcgcaa cgcgtctcag cgagacggca gaagcaagcg aggcggcgcg
     7981 ggcggcgacg ctgcgcatgg atcgcgagct ggctgcggcc aaggcggatc tcgcccgcct
     8041 ctcccaacgc gccgaggcgc tgaaggcgac ggacgaccgg ctcgagcaac ttctgcgcgc
     8101 cgtgcaggaa gaggccggca gcttgcgcag cacggtcgag ggattgcagg gcgagctagc
     8161 cacccagctc aaaaccgtgg cgcgcgccca ggatgtgacc gcagctgtcg cgccggtcga
     8221 aggccgggtc gcggccctcg agcaaaacgt gcaggctgtg gtccgcagcg aggaggaccg
     8281 ccgcgccaac gcggagcgca tcgtccttgc cctcgaactc ggaaatctga agcgcgcgct
     8341 ggaccgcggc ggcagctacg cggccgagct cggcgagctg agacggatca gcgccggcaa
     8401 gatcgatctg gcggtgctcg agcgttacaa ggacgagggc gtaccgactc tcgccgcctt
     8461 gcagaaggaa ttccgccccg ttgccaatgc cgtgatcgac gctgcaagcg tgccggccga
     8521 tgcctcgccg ctggaccggc tgctgatcgg cgccaaggcg atcgtccgtg tccgacgggt
     8581 cgagcacagt ccggacgaca cgagcgtcga ggcgatcgtc ggccggatgg agcaggcgct
     8641 cgccgaaggc aagttcacgg atgttttgga tcaggccagg ctgcttccgc ccaaatctct
     8701 ggagccggcc gaagcatggc tcgccaaggt cgaggcgaga gccgccgtcg atcgcgcgct
     8761 cgccagcgtc gaggatcagc tcaaggcctc gctcagcggc caggcagctg tcgagaagag
     8821 gaccaactga tgctgcgtct ggttctgttc ctgctcgccg tttcggcgct cgccttcggc
     8881 ttgtcatggc tcgccgaccg gcccggcagc ctgatcgtga actggcaagg ctacgagatc
     8941 gaaacgagtg tcttccgcgc catcgtcatc ctcgccgtgc tgctgggact cgccatcgtg
     9001 ctctggtctg ttctgcgcca gatctggcac agcccggccg ccatcggcag ctacttcaac
     9061 cggcggcgcc agaagcgcgg gcttgaggca ctgtcgagcg gcatgatcgc gatcggcgcc
     9121 ggcgaccgcg cgctcgcgac ccgttacgcc atccaggctc gcaagtcgct gccgaacgag
     9181 ccgctgacgc acctcctgcg cgcgcaggcg gcgcaacttt ccggcgaccg cgcaacgtcc
     9241 cggcggatct tcgaggccat gctcgcctcg cccgacaccg agcagctcgg gctgcgcggc
     9301 ctgttcctcg aggccgagcg agagggcgag caggaagcgg cacgccaatt cgccgagcgc
     9361 gccatggcgc tcaatccgaa gctcagctgg cccgtggaag ccctgttcga catccaatgc
     9421 cggaagggcg actgggctgg cgcgctggag acactggcgg tcgcgaagcg gcaccacaac
     9481 atcgagcgcg ccgttgccga ccggcggcgc gcggtgctcc tcaccgccca ggcacaggag
     9541 ctggaggaga ccgatcccga gcgcgccatg actctggcgc tcgaggctca caatctggcg
     9601 ccggacctcg tgcccgctgc ggccatcgcc ggccgcgtgc tcgcggcgaa aggcaacatc
     9661 cgccgcgccg cgaaggtcct gcagcgcacg tggaagcgct cgccgcaccc ggacatcgcc
     9721 gtggcatatg cctacgcgcg gatcggcgac agcccgcgcg accggctcga ccgggtgaag
     9781 cagctggcgg cactgcggcc gcattccatg gagagcgcca tcgccgttgc gacagccgcg
     9841 atcgaggcgc aggactggcc gacggcccgg catgtgctgc agcccttgct cgccaacgcg
     9901 ccgacgcagc gcacgtgcac gctcatggcg cgaatcgagg ccgagcagca tggcgaccgg
     9961 ggccgcgtgc gtgagtggct cgcaagggcc gtcaatgctc cgcgcgatcc tgcgtggacg
    10021 gccgatggtg tcgtctcgga gaagtgggcg ccgacgtcgc ccgtgacggg cgcgctggat
    10081 gccttccaat ggcgtgtgcc cgtcgaggcg gtgaatgctt ccgaggcgac cctgctcagc
    10141 cagaagctcg aagagctggt caatctccat cctgagccgg aggcgatccc gcaaggtccg
    10201 ttgatcgatg ccccgtcagc ggctcctgtg gcggacgagg cgccggaaac ggacgccgaa
    10261 gccgcggcag aagccaaacc ggccgcgccg ggaccgcaga agacatctgc gccgcccgct
    10321 tcggctctgc ccgccgagcc tggtccggaa gctcaggaac ccgagtctgc tgcgggtccc
    10381 gggccagcag ctccatccgc ggaggccgcg cccacggctg cgccctcgcc gaagaagcct
    10441 gccgaggccg ctcaaagtcc tgcaccagag ccggcgccgg ccaaggaagc agccccggcg
    10501 aagacgacct ctggcgccga aagctcatcg gcttcggcaa ggcccaccga gcagcccact
    10561 cccgaggccg ccgcggcaaa agcgctatcg ccggacttag acacacccgc gcccggtcag
    10621 cctgcgccgc ccgcgcacat gggcatcaag acctatcccc agccgttccg cacgcgcaca
    10681 cggccggtcg agccgcgcat cttcgtgccg ccgcgggctc ccgacgaccc cggccccgat
    10741 gcggacggcg aggaggacct gagccgctct cgcgtcggat tccacctgcc gatcaagggt
    10801 tccgtctgaa cagcggccgc gagctgcgat gagaggtcca gcgcccttgc caaggcaggt
    10861 caagtgagta tatgcctgcc gcgtcctgat ccgccgcaat agctcagccg gtagagcaca
    10921 tcattcgtaa tgatggggtc gggggttcga atccctcttg cggcaccagt tccccaaggg
    10981 tccattctcg tcgaatggtt tacattttat tccggagaga tggttgacac tttctccggg
    11041 gtgaatgggt tcggtccggg ttcgacgcgg tcttcctgtc gatcgaagaa gccgagatca
    11101 caatccatga agccatctgg cctggcctat cgctccccga caagcagaag acgtcggcaa
    11161 ccagtgccta tcctgacgag cttggccacg ccgcgcgcat ctttctccaa tccccatcgc
    11221 cgccgccaat ccttctctcg ctgacgaagc ccgggccaac gctgcccaag ttctttggcc
    11281 cgagccccta tgccgcgctg ctttcaacac tctcctttca acaccatcct gcgccgggca
    11341 caggagatca gcccacattg cgattgccag cctcgcgcca cccgattttt gctcaggctt
    11401 gccgcgaatg ttgctcaggc ttgccgtgaa gcggcgagca ctgattgatt ccggtcaatg
    11461 ttcgacattc ggctctctcg ataatcgcac cagcagagtt ggcgggaacc actcaattgc
    11521 gccggctcgg agcaacgctg gtgcttggct ttccagatcg caccggcgct ccggtccggg
    11581 ttcccggtgc tgacccgctc gcggatgccg aagcttggga gtcttcacga tgaaaccttc
    11641 agttctctta tcgtcccttc tggcgctgag ccttgttgcg ggagcggcaa cgcaggtgac
    11701 agcccaacag cgcggtcccg gctggggcat gatgggcgga tacgggatga tgcacggtcc
    11761 cggttggggc cgcggattgg ggatggggtt tggcaattgc ccgatgtggg acgatggcgg
    11821 cgcacctacg ttcgcgcaag gtaggatcgc attcctgaag gctgagcttt ccatcacgga
    11881 ctcccaggca tctgcctggg aagcatacgc gaatgccctc aagaaaaatt ttgagagcat
    11941 gcagggcatg cgtcagacga tgctggcagc gtggcaggcc aagtctcccg tcgaacggct
    12001 caatgcccat atcactgcca tggagggccg gctcgccgcg ttgaaggaca tcaaagcgcc
    12061 actcgagacg ctctacgcag ccctcaatga cgagcagaag aagaaggctg acgatcttct
    12121 gacgggtatg ggctgtatga tgtgagctac aggcggatgc cctggcggga ttgcagggca
    12181 tccgtcaggc cattcaccgc ttcagcagcg gtgggcccca ttcatcggcg tttgcggccg
    12241 gtgatcatcg cgctcacgag gttttcgccg tgctcgaagc tggcgagaat gacgccggct
    12301 acgtgcagcc caaccaggac cagagccaga ttggccaacg cctcgtgaac ctcttccagc
    12361 cagtgcgcat cgtagaaggc gtcgctggtc atcaggatgc ctgttccggc tgtcgcggcg
    12421 agcacgccga ggagggcgat caccatcgcg ccgcccgcag ggttgtggcc gatatagcgt
    12481 ttggcgcgaa gccggacgct gtcggcgaga tagctgacca cttccgatgg ccggtagacg
    12541 aaatcgctga accgcgcatg ccgcgtgccg gcgaagcccc acacgatgcg gacggcgaga
    12601 agtccggcta tgccgtaccc gacatagatg tgcagccact cgacctcgtc gccggtcagc
    12661 cacgccgttg caaacaatag caccagcgac cagtggaaca cccgaacgag gggatcccat
    12721 acgcggatcg tctccggtaa cccgttggcg gcctccgccg gcggcagatc gccgccggct
    12781 cgggcctttt cagtcaggcc agtcatcagt tgcgagtctt ctgccttagg atttcgccgg
    12841 tcgccggatg aaggtaggct ttcacgcgct ttccatcctt ggtggccgaa acctcgtagg
    12901 cgccgtcgtc gatctcgatc tcgcggatat cgtaaccttg cgccgtgagc ttctcggcga
    12961 tctgggcgat cgacagccac tgctccttgg gaacgttcac gcggacatcg tcgtcggagg
    13021 cgacggccaa accggctgcg gtcgctgtgg cgagaacgag tgcggacgtc atcaaagcct
    13081 tgcgcataat ttcgtctcca tttcctggcc ggcacggaat tgtgcggcgc gcaaggacca
    13141 tggcagactg ccgctgacac cttcctgatg atccatgtca gtgaattgtc agctcggctg
    13201 cggcagacag tgagcgatga gaacggaact cgacagacgg acgcttctgc tggcgcttgc
    13261 agccctgggt acgggttcgc ccgccctcgc gcggggcgat gacgaccgca gccaccgcaa
    13321 gcgaagcagt cgtcacgatc acgagcaggc caggcgcgcg ctcgaggagg gtcgcgcgcg
    13381 gccgctggcc gacatcctcg ccgaggtccg tccccgtctc ggcggggagg tcatcgacgt
    13441 cgagttcgag cgcgaggatg gccgctacgt ctacgagttc aaggtgatca cgcccgcggg
    13501 ccagctgcgc gagatcgagg tcgacgcact gacggcggag atcctcaagg acgaggcgga
    13561 ctgatgcgca ttctggtggt cgaggacgag ccgcgcattg cagccgatgt gcgtgccgcg
    13621 ctcgaggccg ccggatatct cgtcgacatt gcgcgcgatg gcgacgaggc gtggttccgc
    13681 ggcgataccg aggactatga tctcgtggtt ctcgacctcg gcctgcccaa aatcgacggt
    13741 ttggcggtgc tgaaacggtg gcgtgccgcc ggccgcgcca tgcccgtgct ggtgctgacg
    13801 gcccgcggct cctggtcgga gcgggtcgag ggtatcgatg ccggggcgga cgactacctg
    13861 cccaagccgt tccgcatgga ggagctcgtc gcgcgcgtgc gcgccgtcat ccggcgctcg
    13921 gccggtcacg ccgattcggt gatcgaggtg gggccgctga cgctggacat tcggcaaatg
    13981 cggctcacga aggacggcgt gccggtcgcg ctctccccgc tcgagtatcg ccttgtcgcc
    14041 tatctcatgc atcacaaggg gcgtgtcgtg ccggggccgg agttgctcga gcacctttat
    14101 ggtgacgatg atgcgcacga ggcaaatgcg ctcgaggccg tcgtggcgcg cctgcgcaag
    14161 aagctcggac ccgatgcgat cgaaacccgg cgcggtttcg gctacctcat cgctgccgag
    14221 gacgcatgaa cggcggctcg cttcgacttc ggctctttct tggcggagcg ctctcgatca
    14281 tagcggcatt ggctctggca gcgctggggt tggttctcct gttcgagaag cacgtggagc
    14341 gccgcatcga gagcgagctc ggcgtctacc tcgatcagat catcgccaac ctggaccgtg
    14401 atggcgacgg agcgctggtt gtggccgatg tgcttgcgga cccccgcttc gaccagcctc
    14461 tgtcggggct ctattggcag gtccgggcga aagatgccgt cttgcggtcg cggtcgcttt
    14521 gggatggcga tctaccgttg cccagcgaca agttggccga cggcgtcgtt catcggcatc
    14581 ggatcatagg accggctggg gcggagcttc ttgccctgga gcgcagcgtc agactgccgg
    14641 cgcggcttgg caatgcctct gttcgagccg cggtcgcact cgatacccgc gagatcgcag
    14701 cggccacccg ggcgtttgcc gccgatctgc tgccttacct cggcgtcctc gcggcggtcc
    14761 ttatcgcggc cgcatacgct caggtcacgt tcgggctcag cccgctcgca accattcgca
    14821 agcgcctcgc aggcatccgc gagggcaacg cgacgcgcct cggcaaagcc tttcccaagg
    14881 aggtgcaacc cctggctgcc gaggtcgacg ccctgctgga ggcgcgcgag caagaagcgg
    14941 aacgggcgcg gtcgcgcgcc gccgatctcg cccatgggct caagacaccg ctgcaggtgc
    15001 tcgccggcga cgtggcgcgg ctcaggcaga aaggggaagc ggacatcgcc gccgagatcg
    15061 agcaggtcgc cacggctatg cgccgccatg tcgagcgcga gctggcccgc gcgcgcatga
    15121 aaagtggcgt cagcacggct cgcgccaacg tccgcgaggt gatcgaccgc gtgctcgccg
    15181 tcgtgatgcg gacgcctgac ggcgctcggc tcgcgtggac tgtcgagtgc ccaccgcaac
    15241 tcctggcgcg gatcgatgcc gacgacctgg cggaggcgat cggcaacctc gtcgacaatg
    15301 cagccaggca tgcgcggcgc gccgtccgcg tggaagcgcg ggcagaggac aggcgagtga
    15361 aattgctcat ctccgacgat ggtcccggga ttccgccgga gcggatgagc gaggttctcg
    15421 cccgcggcgg gcgccttgac gaatcgggca cgggagccgg cctcggcctc gccatcgtgc
    15481 aggagatcgc ggacgcctgg gccggccggc tggagcttcg gccgaacggc cccgggctgc
    15541 aggccgagct ggcactcgat ctggtgcggg gtccttgatg catggagcgc gtcgcggggc
    15601 gccttggtca aggttggcga tgcgcttcgg ccttcttcca gtccggacgc gtgtcggcgg
    15661 attgctgcgc gcctcaggcg agaaaccgga atggcttggg tcgatgccgg atgccggtca
    15721 cttggcggcg tcaccgggag cacgggccgg acggctcatc gtcatggccg gcggtgaggt
    15781 ggagtcgcaa agcttcggct acatgggcca caaggcgcgg gcatcaccaa gctgggcaac
    15841 caggtgatct attcctgcac caaggtggtg ctgtcgggga tgctgatgct ggcccgcggc
    15901 cggtgggtat ccggacggac cctgcgttgc tgccgctgga tcgcaatgtc gagacggggg
    15961 ctggcacaag cgcggcccgc cgcttagccc gcgagcttac tgagttctgg cggccgacgg
    16021 cgcagctgcc gtcgatgctc tcgccttggc aacgggcttt cgcccgccct tcttgacaac
    16081 ccgcgccgga cgcctgccgc agctccacga ggtcacggta tcgcgcacac tcgtcagcgg
    16141 ccttgggttt tcagagaccg gcagcgtatc gagcgtcgtc gtgccgaaca gaaccttcca
    16201 atcgtaggtg gcgaacccgt gatcgaggag attgctcgca cgctgggcgc gctcctggct
    16261 cgacggctcg ccgagcacga cggccatgag ctgccgcccg tcgcgggtcg cgctcgccac
    16321 tatgttatag ccggagtcgc agatgaaccc tgtcttcagg ccatcggcgc cgtcgtacgt
    16381 cttgagaagg ttgttgtgcg tcgcgatgcg gcgcttgccg atgcggaatt cggccagccc
    16441 ccacaggtat cgataatccg gaaagtcgcg cgtcaccgcc atcgcgagct tggccagatc
    16501 gcgcgccgtg gtagcctgcc ccgcagccgg cagcccattg gcgttgacga aatgcgtatt
    16561 cgtcatgccg aggcgaagtg ccgttgcatt catgcgctca acgaaggcag gctcgcttcc
    16621 gccgacggct tccgcaagca tgatggcgac atcgtttgcc gatttgacga cgagggactc
    16681 caggcccagc tggacgctca tctcgccgcc caccggcagc ccgatcttgc tcggcggctg
    16741 ggcatgggcg gcttcgctgg tgacgagctt cgtctcaagt gtgatctgac ccgccttcaa
    16801 cgcctcgaag acgacgtagg cggtcatcat cttggtcaat gaggccgggt accactggtc
    16861 atcgatgtcc tcggcgtaga gaaccttccc atccgccgga tcgaagagca gcttcgggcc
    16921 ggcttgagcg gctgccggca gaaggagacc ggcgcacatg agggcgatcg agatggtccg
    16981 ggaagaaatc atccagccat tcgctccgat acatggatgc cggatcgggc catcgccaac
    17041 gcacgccact tcgcacaaag ccctgtcgca gacgccctgt catcggcagc aattggctga
    17101 agtgtccaat gccttttccg gcgagccaat cctactggtg ccaagttacc cttctctcta
    17161 cgcgaggtcg accgccgagc cggtgatttc gggaagcatg gcaaccttcc agtcatcccc
    17221 tgttctggcg tctgctcgca agtacgtcaa cacgaggtgg gctctgtcgt tccgagcgtg
    17281 agatatgggc aacagatcat gcaccagaaa catgggccgc aagagcctct tcgatgcagt
    17341 ctccgaccag agagcgtggg acgacagacg accccgtgct caagcccccc gcgaggcacg
    17401 cggaaggaag aaggccagca gcccgagggc cggcagccac gcacatatct tgaacacggc
    17461 aatgatgccg atctggtcgg cgagcaatcc gatcgccgct gccgccaacc cgccgaggcc
    17521 gaaagccaaa ccatagaaaa gtccaccgac gagacccaca cgatgcggca ccaggtcgat
    17581 cgcatagatc aggatcgatg cgaaggcgct cgccatgatg aggttgatca ccacgctgag
    17641 cacaccggtc cagaacaggt tcgcgtaggg cagcagaata gcgaacggca gcgttcccag
    17701 gatggagaca aggatgacct gatagcggcc gatccgatcc ccgatcatgc cgccgacgac
    17761 cacgccgatt gcgccgacga agagatagag gaacagcatg atctgcgaaa atggcacgct
    17821 cactccgaac cgctcgatca gatagaaggt gtaataggac gtgaacgcgg ccgtataacc
    17881 gttttttgac aggagaagga cgatcaggat cgccatcgcc atcagcacat ggcccacagg
    17941 caagctccga gcagccgcgc cggttgttga tgattttcct gccgcccttt gggagcgacg
    18001 cattgcggcg tatcgccatc cggtccaggc cataagcacc atggcggtga gggcgacgac
    18061 ggagatccag gcgaggcttg cctggccatg cggtacaacg atcatcgcgg cgagcagcgg
    18121 gccgattgca tatccggcat gtccgccgat ctggaagata ccctgcgcga aaccctgctc
    18181 gccggccgcc gcatgccgcg ccatccgcgt ggcctcggga tggaacacgg ccgatccaag
    18241 cccgaccatt gcagccgaaa cgagtagcgt cccatagctg gtggcgaagg caagccctag
    18301 gagccctgac aaggtggctg ccatacccgc caccatagcg tagggccacg gcctatggtc
    18361 ggtcacgaaa cccagcagcg gctgcaaaag ggacgacgtc acctgaaagg cgaccgtgat
    18421 cagcccgatc tgcacaaagt ccaggttgta cgtctgcttg aacagagggt acagcgccgg
    18481 gatcatcgac tgaatgaggt cgttgagcag atgcgcccca ctcagagcga aaagaatcgg
    18541 cagtagggga cggtgcggag cactggtggc gatcgtcggg gtcatggcgg agatttggac
    18601 ctcgccgatc ttggcgtcaa ctggcgcatt gcgtatggct gaccgggcaa atcaggggcc
    18661 ctcggaagcc gtgtcagggg cctgcccgat caccagaccg gcagccagca gtcaagcggt
    18721 cacctgatcg tccgttccct gcggcgatcc ctcaaactcc gaagaactca cagatggcat
    18781 ccactgccct tcgagcatgc ggattccgcg ctgaatgggg cagccacggg cggcgagccg
    18841 acctggtcga tggtatcgcc gatgatcgta tagagccgcg tctcgccgga gagcggcaac
    18901 ggcaagccgg gcaagtcggc attcgtcatg cgctgaattc tattccacgg tgctcggaag
    18961 tctggatgaa gcaagaatct caacgccaac tcaccgcgta ctctttcaaa tggacagcat
    19021 ccggttctat tccgagccca ggccgatctg gaagtgtcac cttgccatcg cagatcgttt
    19081 ggaccggacc gctgaggaga gttcggagcg gattcgggtt ggagtcgatc tcgagccgtc
    19141 catcgccgcc cgcggcggca aggaggtggg cggaggcgag gaggccgacg ccacctccca
    19201 ggtaatgcgg gcaatatgtt ctgcccgcgg cgatgatccg cttcgctaca gggaagcagc
    19261 cgctgaagcc gccccatttg gcgatgtcag gctggaccac gctgagcacg cccgatttca
    19321 gcgctaaatc gaaggcctcc aagcccatga tattttcccc ggcagccagc ggcacggacg
    19381 tccgctcgcg gagcttggcc cactcgctcc agggctcatc cgccctcagc ggctcttcca
    19441 gccactggag ttgaaacggc tccaagcggg gcagcacatc gagagctgta cggacgtccc
    19501 aagcctgatt ggcgtcgacc atcaagaggg cggccggccc caatgaagta cgcagtgctt
    19561 gcaggttcct gacgtcgcgc tcgattccga agccgatttt cagcttgaag gcgatatggc
    19621 cctccttttg cctggccaat gccaaacctt ccggattggt cgggttcagc ccgcttgcat
    19681 aaacttgtat gttcggcgcg gccccaggat tgagaaattt ccagagcggc tgcccggcgc
    19741 ggcgtgcttc gagatcccag agcgcgatat cgagaccggc gatcgcctgg gcaatcgggc
    19801 ccgcctcacc gctctggatg gccagcactg ccgtacgccg ggtcaggtgc tcaaaagcct
    19861 cctgagccga cgcgaaggtc ttttcgcaag acaacggggc cagcacgctg tcgacgagcc
    19921 gggcgcgatg ctcggctccg accgccggaa aattgcacca gatctcgccc cagccgacgg
    19981 ccccctcccg atcttcaacc ctgaccagca ccatgggccg gtcgcgcatc gtgccaaacg
    20041 aggtcgtcac cggctcgtcg aggggacagc ggtagacgaa cggagtgacg ttgcggatgt
    20101 cgagtgtcac ggtttcagat ccatttccaa gtggatatag tctccgcgat acgtcacctc
    20161 ggcaagatag atcacggttc catccgttcc gcacatgacg cgccggacct ccgcaacggg
    20221 cgcattgatg gaaatcccga ggagttgcgc ggtcgtgacg tccgctgagg atatcgtgag
    20281 ggtctgacgg gccgagctga tctgcaggcc gggtaaggag gtcagaatcg gtatcaccag
    20341 ctcccgccgg aatcgcgaag gcgcgcggcg gaaaatccga tactcgatat agatcgagat
    20401 gacgcagtaa cgctcgccat cccgctcgtg cacccgacgc atatagaagt acttgggtgc
    20461 ttttgccccg tcactctcat ccagcgccgg ttcggcaatt ccttcatcca gcgtcgtgtg
    20521 ttggggtcga tcgccgcgat acatcgtgac caggtcgtcc aacgtcgtct cgacgcgcag
    20581 cttccggcgc ggcccggcct tgctcatcac gaatgttccc cgcccgcgct gcggagatag
    20641 caatccctcc tctgccagaa gcgcgatcgc ctggcgaacc gtgacccgag agacgccgaa
    20701 ctccgccatg agctgctcga tcgacggcag aatgctgccg gggcgccaga accccctgtt
    20761 cacgcgctcc cggaataatt ccgccagctg cacgtagcga gggatcggcg ttgcgccgaa
    20821 gatgtggtgg ctggagtatg acataaacgg ttgccttgaa cgtcctaaac atagtacgtt
    20881 taggacgttt gggccagccc cgctttttca aggattgcca gtctgcccgt gcgtcgaact
    20941 gctttgatcc tcatgccctg gatcgccatc ctggcggtct ggtacgccat tcatcacagc
    21001 ggcctcgtca atcctgcgct ggtccccaca ccgcacgagg tcggccgtcg tttcatcgag
    21061 ctgctccggg gtcgtctgcc gcttgatatc ctgatgtcga cgcagcgcgt cttcctcggc
    21121 gtgctcagcg ggattacgat cgccgtaccg gtgggcttcg tcctgggctg gtatcgcaat
    21181 atccgagcgt tcgtcgatcc gctgatcaac tttttccgtg cgttgccgcc gattgctctt
    21241 attccgctgg tcatcgttta ttttggtgtc gacgagctcg cgaagacggt cattctcttc
    21301 tatgcgtcgt tcttctcggg tgtcatcgtc atgtacgagg gcatttcaca gatcagcccg
    21361 atctacgtgc gtgtcgcccg cacgctggga gccaacgatg gcgaaatttt ccgcaaggtc
    21421 gtcattccgc tcgccgttcc acacatgctg accgccctcc gggtggcgct cggcgtggca
    21481 tgggcgacac ttgtcgcctc ggagctcatt gcggcccagc aagggctcgg cgcgatgatc
    21541 caggatgcag gctcgttctt ccagctcgac atcatctatg tcggcatcat ctgcatcggc
    21601 ttcatcgcgc ttttcatgga ccttgcactt cgcgccatcg cccgcaagtt tgtcagctgg
    21661 caggagcgca tcgtatgaac gccgggcgtg tacgtatcgc cttcgaccgc gtgtccgtgc
    21721 gctttcccac gcaggacaac ggcgaaatct tggtggtcga cgaggtcagc tacgacatcc
    21781 acgaccgcga gttcgtctcg atcatcggtc catccggctg cggcaagacc acgatgatga
    21841 atatcgtggc cggcttcatg cagccatcct caggctcagt cacacttgac ggaaaaccga
    21901 tcaccgggcc gggtccggac cgcggcgtca tttttcagga atacggagtt tttccctggc
    21961 tcagcgtgaa ggagaacatc gcattcggac tgaatttgcg cgccaatcgc accagcgcgg
    22021 cggagcgcga ggagatctgc gagcgctaca tgcggctgat gggcctttcg gaatttgcaa
    22081 atgcctggcc gcgcatgctt tcgggtggga tgcgccagcg gctcgcgctc gcccgtgcct
    22141 atgccgtcaa gccgcagttt ctgctgatgg acgagccctt cggcgcgctc gatgcccaga
    22201 cgcggtcggc aatgcaggac ctcctgctcg aggtgctggc cgccgagggc aagacagtca
    22261 tgctcatcac gcactccgtt gaagaggcgg tttatctttc caaccgcatc atcgtcatga
    22321 cggcacgacc gacgcggatt cgcgagatca tcgaagtacc gttcgcctat ccgcgtaccg
    22381 acgacctgca tgaaagcaag gagttcggcg agctgcgcgc ccatgtgcgc aacctcgtca
    22441 tgcaggagta cgcacagcag gctctgcagc accagactta aaggccgaag ccaacccagg
    22501 aggaaacgat gtcgataaac cgtcgaactc tcataaagtc cagcttggct gttgcgggcg
    22561 cagcggcgat cggcgcgccg gccatcgcgc aatcgaaaac accgatccgc gtgggctatt
    22621 tgcatacgct cgctgtggac ggacagatgt ggctcgccga tcacctcggc gcttatcaga
    22681 agcatggatt ggagcccgaa ttcaaactct tccaaaccgg gctcgagctc tttcaggcca
    22741 tgatcggcgg aagccttgat gtgctcagca ccggggcggt catctccaac ttccccgctc
    22801 gcggccaggg caaaatgttt ctcaccaacg gcgtggagtt cgcaacggcc cagctctggg
    22861 tgcgcgagga tcagggcatc aaatccatcg ccgatctcaa aggtaaaaga gttgccacga
    22921 ccgccggcac gaccgcgcat gttttcctgg acaccgccct caaggcgaat ggtctgcagt
    22981 cgagcgacat cgagattttg aaccagcgta tgcccgatgc cgtcacctcg ttcatttctg
    23041 gatccgtgcc cgccgttgcg ctgtgggtgc cgttcaacat caccgtgcgg gataaagtgc
    23101 cccaggccaa aaagctcatc gatgcgtctg ccttttatcc agacgctgcg atcatgtccg
    23161 gttgggcgac gacggccgat ttccatgcga agaatcgcga cacgctcatc cggctgatcc
    23221 aggcctgggc cgaaggcaac gagtatctca ctggcaagac ggacgaagct ctcgaaatcc
    23281 tgcagaaaaa gcactaccca caagtcccct tggccgactt caaagagcag tacgccgccc
    23341 aaaagagctt ccccgtcgcc gagtggcgca agctctatag cgatggcacc gtgaccaagt
    23401 ggcttcagca ggtgacagat ttctttgccg caaccgggaa cattccgaac ccagtgcccg
    23461 cgtccgagta tttcgacccg acgctgttca tggaagcgac aaaggcctga tctttgccga
    23521 accagacgta cgactacatc atcattggag ccggttcggc gggctgcgtt ctcgccaacc
    23581 ggctttctgc tgatccgagc tgcaaggtct tgctgctgga gtccggaggc tgggaccgca
    23641 acttctggat caagctcccc gtcggctatt tccgtaccat ttacgatgag aggttttcac
    23701 gccttttccc gacggaaccg tcggaaggca cggctggccg cggtatcgtc tggccacgcg
    23761 gccgcatcgt tggtggctcg agctcgatca acggtctgat tttcatccgt ggccagcggg
    23821 agggcttcga cgactgggaa tctctgggag cgtccggctg ggggtacgag gacgtgctcc
    23881 cttatttccg caagctcgaa tgcttcaatg gcgagcctag tcagtatcgc ggagcgcacg
    23941 gagagctgcg cgtttcgata ctgcgcaacg atcatcctgc gtgccgggcc tggatcgacg
    24001 ccgctcagca attcggcctg ccgtacaatc ccgatttcaa tggcgaaacc acttacggcg
    24061 tcggctcgta tcaactcagc atcggcccca gatggcgaga aagcgcggct gttgcattcc
    24121 tccggcccgc gctgtcgcgg ccgaatttga ctgtcctcac acgggcacac gcgacccgga
    24181 tcgcattcga gggtactcgc gccgtcggtg tctattgggt tcaacgaggt cgcgaggaat
    24241 tggctcgtgc tgagcaggaa gtcattcttt cggccggagc tttgcagtcc ccccagctct
    24301 tgcagctctc tggcgtcggt ccagccgatt tgctgcgcca gcacggcgtc cccgtgattg
    24361 ccgatttgcc gggcgtcggc gaaaacctgc aggaccatta tcaggcccgc accatcgtac
    24421 gcctgacctc gccaatctcg ctcaacgacg acgtgcgcaa tccgctcagg ctggcgcaca
    24481 tgggttggag atggctcgta cgcggggaag gcccgctgac gatcggagcc ggtcaggttg
    24541 gcggggccgc ctgcacgcaa tatgcggttg gtggtcggcc cgatatccaa ttcaacgtga
    24601 tgccgctttc ggtcgacaag cctggagacc cattgcaccg ctattcgggg tttaccgcct
    24661 cggtctggca gtgccacccg gtctcacgcg gccggctcac gatccgcagt tccgacccgt
    24721 tcgcaccgcc gcgcatcgag cctcgctacc tcgatgccga gctggatcgc aaggtgatgg
    24781 tcgaaggcgt gaagatcctt cgcgagatct atcatcagcc ggcattccgg aaattatgga
    24841 cagacgaagt ccggcccggt cttgccgccg tcgccgatga ggccatcctg gccttcatcc
    24901 gcgagcaagg cggcacggtc tttcatgcct gcggaacctg ccggatcgga aatgacgatg
    24961 gagccgtcgt tgattccttt ctgcgcgtgc gcggcgtgga tcggctgcgg gtggtcgatg
    25021 cgtcggtcat gccgctcatc acgtcggcca ataccaacgc ggcatcactg atgattggtg
    25081 agaagggcgc cgccctcatc aagggtgagg ctcgcgctcc ctcctgagcc atccgctgac
    25141 gccacagccg g
//
LOCUS       DASVDU010000020        16602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_733309, whole genome shotgun sequence.
ACCESSION   DASVDU010000020 DASVDU010000000
VERSION     DASVDU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16602
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733309"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VF226_01575"
     CDS             complement(<1..791)
                     /locus_tag="VF226_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7072706.1"
                     /translation="MAQIDLDYNATTPVDPEVLDAMLPFLQGEFGNPSSSYPLGKRAR
                     DAVEEARARVASLIEAAPEEIIFTGGATEASNLAIRGAVALGHGRSNIVTSNIEHPAT
                     AACCDLLERSGYGVRRVPSQRNGIVSSDDVASAIDDQTAVVTIIHAQNEIGTIQPVAR
                     ISREAKRRGALIHTDAAQSVGKIPVSVRELGVDLLTIAGHKLYAPKGVGALYIRKGGK
                     LPPLIVGAGQEHGIRPGTENVAFIVGLGAACAIASRRLDETRKRMA"
     gene            complement(857..1963)
                     /locus_tag="VF226_01580"
     CDS             complement(857..1963)
                     /locus_tag="VF226_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX7072707.1"
                     /translation="MMVAVNRRDVLRAGAVLGAATLYPGLASAQSTFAPRPGAWRTFE
                     TVTRLEISKPAGVVQAWVPLPAFSDPEWFRPGGSTWTTNAKTAEIKRDSKYGAEFLHV
                     VWAEGEPAAIEVTSKFSTRDRAIDLSKPGGAPSLSGADRELYTSATELIPTDGIVKET
                     SDKITAGASGDLEKARRIYEWIVENTHRDPNTRGCGIGDIASMLKSGNLGGKCADLNA
                     LYVGLARAAGLPARDIYGLRVAPSKFGYKSLGAGSEVVTKAQHCRAEVYLSGFGWVPV
                     DPADVRKVILEEPPGNLSISDDKVAAARKALFGSWEGNWLAYNVAHDLDLPGAKGPTL
                     EFLMYPQAETAEGRLDCLDPDTFTYKITAKEVTA"
     gene            complement(1991..2533)
                     /locus_tag="VF226_01585"
     CDS             complement(1991..2533)
                     /locus_tag="VF226_01585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7072708.1"
                     /translation="MLYLTLVASVSAVLADASGFYFEVSKTSAPHPQLQAVAGKETPA
                     LTLDDIHGSRHKLEQSAGKTILVHFFATWCEPCRPELASLSKLLARDKGRSLAVLAVS
                     VAEPPARVKRYFETSPVEFPVLLDADRSTTKAWGVSVLPTTFVVDPTGAIRLQVEGDL
                     DWQRPDVLALLENMGACHSN"
     gene            2837..5911
                     /gene="fdnG"
                     /locus_tag="VF226_01590"
     CDS             2837..5911
                     /gene="fdnG"
                     /locus_tag="VF226_01590"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:3434..3436,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEX7072709.1"
                     /translation="MNLEVSRRHFMKLAGAGVAGSAVTAFGFGEAEAQLAAHVRPYKL
                     AQTKEARTICPYCAVSCGMMVFSAPNKANGGKLEVTHIEGDVDHPVNRGTLCPKGSAA
                     IDYIRSKSRVKYPMYRKPGSNTFERVSWDFAMDRIARLMKDDRDANFIERNKDGVIVN
                     RWTTTAFLSGSSLTNEGGWLTYKVARGLGMLQLENQARIUHGPTVSSLGPTFGRGAMT
                     NSWTDIKNADVIWIMGGNAAEAHPCGFKWVTEAKHHRGAKLMVVDPRFTRSAAVADHY
                     APIRAGSDIAFLSGVVNYLLTNNKIHTEYVRHYTNASYLVREGYAFNEGLFSGYDEDK
                     RAYDRSTWEYEIGPDGYAVRDMTLQHPRCVFQLMKQHFAPYTPEVVSRISGTPQDKFL
                     RVCEWVASTANGERSMTIMYALGWTQHSHGAQNIRTAAMIQLLCGNIGIPGGGVNALR
                     GHSNVQGITDVGTLTAAIPGYLALPTENEPTLESHLAKRKFTPLQPNQTSYWQNYPKF
                     FVSFLKSFYGNKATPANSFGYDWLPKLDVAYDCLRMFETMFQGKTNGLICQGFNPVMA
                     IAHQKKSTAGLSKLKFLVSMDPIETDTARFWENHGEANDVDPSKIQTEVFMLPVTTFA
                     EEEGSLTNSSRCIQWKWRAADPSYEAKPDIAILADLFMRIRKLYEQEGGKGAEPLLNI
                     AWDYANPLEPTADELLKELNGKALADIRDAEGNIIRRAGEQLASFGEMRDDGSTDGSM
                     WIYTGVYGPNGNNSQRRDNSDPSGLGVYPNWGFSWPANRRIQYNRASADPQGRPWSEA
                     KKYMYWDGSRWTGPDVPDYVPTIPPERATGPFIMNPEGVSRLWVRGLMADGPFPVHYE
                     PFESPVENPLFPKIKGNPAARVFATDLDVFGDPKEFPIVATTYRLTEHFHYWTKMVHM
                     NAVMQPEFFVEMSEELAREKGIKKGDMVRVWSNRGSVKGKAVVTKRLKPLTINGKVVH
                     SVGLPLNFGFIGETKKVAPINSLTPPVGDANAQTPEFKAFLVDIEPVRGPVA"
     gene            5926..6867
                     /gene="fdxH"
                     /locus_tag="VF226_01595"
     CDS             5926..6867
                     /gene="fdxH"
                     /locus_tag="VF226_01595"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEX7072710.1"
                     /translation="MYPPIANPTTEPTPPKFGENDLIRRSASTVTPPAQRQTEVAKLV
                     DVSKCIGCKACQTACLEWNELEEEVGVNVGVYDNPHDLTANTWTLMRYTEWENPETNN
                     LEWLIRKDGCMHCEDPGCLKACPSPGAIVQYSNGIVDFIQENCIGCGYCIKGCPFNIP
                     RISPVDNKAYKCTLCSDRVAVGQGPACQKACPTQAIVFGPKDEMKKWADERIKDLKSR
                     GFENAGLYDPPGVGGTHVMYVLQHADKPELYAGLPNNPKISPVVEAWKGLSKYAGMAV
                     LGLTAAAGFVHYILQGPNRVTDEDEQNADRLAGGNSQ"
     gene            6864..7544
                     /locus_tag="VF226_01600"
     CDS             6864..7544
                     /locus_tag="VF226_01600"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX7072711.1"
                     /translation="MSQAKTSAHLRVRRYSGSARINHWIVAICFVLLMLSGLSLFHPS
                     FYPLSALFGGLQIAREIHPWIGLVLVVAFLGLFIRFFPQNLPETTDFVWLARLRHVLT
                     ANDKFLPEVGKYNAGQKFVFWSQFVLIGVMLVTGIGLWSEGRAYVEELFGFTTTIEQQ
                     RWAALLHSSAAVLAISVWIVHVYAAIWVRGTISGMTRGTVTGGWGWRHHRKWLRKEVE
                     SGGAKKVA"
     gene            7604..8521
                     /gene="fdhE"
                     /locus_tag="VF226_01605"
     CDS             7604..8521
                     /gene="fdhE"
                     /locus_tag="VF226_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX7072712.1"
                     /translation="MTETGSAKLHSVNIGEEAKPPAAVLPYPASLFLNRSKRLLALAP
                     GHQLEPYLRFLSVVTHAQHDIQAALPDASVPPPEQIAQAIEHGMPPISRFTLKPDDVS
                     QTTVDHLLENLAQRQLSAEAAAAVQTWRSMTGEQRRAAMADAFSEADSQDSIAFRALV
                     LAGLQVHFTRMAAKLDAASLKPIADGACPACGSPPVASSVVNWPGATNTRFCTCSLCA
                     TMWYVVRVKCLLCSSTESISYRLIDGYPDTIKAETCDKCHGYVKIFYQVKDPVLEALA
                     DDVASLGLDMLLTKEGWKRGGHNPFLIGY"
     gene            8564..10006
                     /gene="selA"
                     /locus_tag="VF226_01610"
     CDS             8564..10006
                     /gene="selA"
                     /locus_tag="VF226_01610"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX7072713.1"
                     /translation="MNQALPISPTRLPSVERVLRTDVGAAAIARFGHEATVDAVRRVL
                     DQVRQARGSAIIALPDKRAVAAEALELLEREHAPSVRRVFNLSGIVLHTNLGRAVLPE
                     AAIEAAIMAMRQPVALEFDVGSGKRGERDDHVRGLICELTGAEDAAIVNNNAGAVLLV
                     LNTLAAGNDAIVSRGELIEIGGSFRLPDIMARAGARLVEVGTTNRTHLRDYEQAIGQH
                     TGLILKAHTSNYLIQGFTKAVPASELAALARRHNLPFVNDLGSGALVDLSRYGLRPEP
                     TVRETLSEGADLVTFSGDKLLGGPQAGIIAGRKDLLERVRKNPMKRALRIDKIRLAAL
                     EAVLKLYRDPGRLHETLPTLKSFVRPQKDIEVAATRLLGPISAAVGSTYDVGIIECSS
                     QIGSGALPLETLPSAGIAMTPADRKGAGTRLEALAAAFRALPIPVIGHIRKGAFVIDI
                     RCLEDEAAFLAQLDQLKNSIGKGVRCDAAG"
     gene            9993..10787
                     /locus_tag="VF226_01615"
     CDS             9993..10787
                     /locus_tag="VF226_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7072714.1"
                     /translation="MLQADSRRDERDETDRRAERMAHAAEAAARGDYATAQGIWMVYA
                     QAGYARAQAEMGQCFLHGWGVERDPDLACFWLTLAANAGDPVSQSLLGDFYFNSEAGR
                     PQPAIAEKWYVRAAKQGDPHAQDMLSRILMEGRHRKPDYKQAHEWALKAAEQGVAAAM
                     TRLGLLYNQALGVERDPKSAAEWWRKAAINGDADGQAMLGAAYHLGAGIERDRVAALA
                     WLTRARRARSKLADPYYPVVRQSCSPDERREAERLASLPLGVEARS"
     gene            10784..12615
                     /gene="selB"
                     /locus_tag="VF226_01620"
                     /pseudo
     CDS             10784..12615
                     /gene="selB"
                     /locus_tag="VF226_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
     gene            12725..12820
                     /locus_tag="VF226_01625"
     tRNA            12725..12820
                     /locus_tag="VF226_01625"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12759..12761,aa:Sec,seq:tca)
     gene            12928..13305
                     /locus_tag="VF226_01630"
     CDS             12928..13305
                     /locus_tag="VF226_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072715.1"
                     /translation="MAEEITRLERQRAFAFRRLNLMREVAKAIASAENEESAIACGLA
                     VVRTELGWETENESRTETLNHFAPVVRAAFEGLCPPETGEATGNVAEALSGFEDWYES
                     ANGKPFWVLFDQYVPELPLVERC"
     gene            13382..13759
                     /locus_tag="VF226_01635"
     CDS             13382..13759
                     /locus_tag="VF226_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX7072716.1"
                     /translation="MKKKLLIVLVNTDPRNPEELAAPFYHAAVAAAMDIEVDVVCAAT
                     AGKLMRKGVAEAIRVKPTHDKTVYDWICDAHRTGARFWACPANLDLFHMTEEDLIPEC
                     KGLMGAATMLENMMSEDCRVLSY"
     gene            13768..14196
                     /locus_tag="VF226_01640"
     CDS             13768..14196
                     /locus_tag="VF226_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072717.1"
                     /translation="MVLINGKEYPEHFYYDLDDQIWYEPLEDGTVRVGFTPAAMDLAG
                     EIFVFTPKRNGYRFEAQRSFAIIEGGKWVGAAHAAFAGVIVNANSELQRRPALLNEDP
                     FGAGWMLTMRPEDENWRDRLLTGPEIGPAFSAWIAGSRHT"
     gene            complement(14313..14386)
                     /locus_tag="VF226_01645"
     tRNA            complement(14313..14386)
                     /locus_tag="VF226_01645"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14352..14354),aa:Gln,seq:ctg)
     gene            complement(14557..15333)
                     /locus_tag="VF226_01650"
     CDS             complement(14557..15333)
                     /locus_tag="VF226_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072718.1"
                     /translation="MDMQIEGLRILVTAGAAGIGLEIARAFVREGARVHVCDIDKAAL
                     EALAKTDPKLTWSVCDVADRDQVAAMFKEALEQLGGLDCLVNNAGIAGPTGRVEEINP
                     EDWDRCLAVDITGQFNCVRLAVPHLKQSKNASIMNLSSQAGKHGFPLRSAYAAAKWGV
                     VGFTKTIAMELGPFGIRANALLPGLVAGDRIRRVIEAKAQQRGVSFKEQEEHMMQYAS
                     LKEYVTPQQLADMVVFTASPRGRTISGQAISVCGDTNMLG"
     gene            15446..16384
                     /locus_tag="VF226_01655"
     CDS             15446..16384
                     /locus_tag="VF226_01655"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX7072719.1"
                     /translation="MPKVAILGAGLIGRAWSMVFARAGWDVTVWDGVAGVAENALSLI
                     AQGLQDLRRHELIDDPEAALARVSAAGDLENAVTGADLVQESTPETLEMKREIFARLD
                     SAAPPEAILASSTSSLVTSLYTEDLAGRSRCLVAHPVNPPHLVPIVELCGAPWTSPEV
                     MQRAREIYRSVGQVPIHVKREVDGFVLNRLQTALLAESFRLVEEGVVTPQDLDETIRN
                     GLGLRWAFMGPFETIELNAPGGIPDYCRRYSPFFRRLADDQPKASVWDDHRTAEAAAA
                     WGPIPDPQKHAAKTRWRDEQLAALAAHKRRMKPFTD"
     gene            16397..>16602
                     /locus_tag="VF226_01660"
     CDS             16397..>16602
                     /locus_tag="VF226_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX7072720.1"
                     /translation="MSKSRKVIITCAVTGSIHTPSMSPYLPITPEEIADAAVGAAEAG
                     AAIVHLHARDPQTGKPDQSVEAFAP"
BASE COUNT         3230 a         5050 c         5180 g         3142 t
ORIGIN      
        1 gccattcgct tccgcgtctc gtcgagacgg cgggaggcga ttgcgcaggc tgcgcccaaa
       61 ccgacgatga atgcgacatt ttcagtgccg ggccgaatcc cgtgctcctg ccccgctccc
      121 acaataagcg gcggcaactt accgcccttg cggatataca gcgcgccgac cccttttggg
      181 gcatagagct tatgtcccgc aatggtcagc aggtcgacgc ccagctccct gacgctgacc
      241 ggaattttgc ccaccgattg cgcggcatcc gtatgaatga gtgcaccgcg ccttttcgct
      301 tcgcgagaaa ttctcgcgac tggctgaatc gttccgattt cattttgcgc atggatgatc
      361 gtgacgacag ccgtttggtc atcgattgcg cttgcgacat cgtccgagga aacgatgccg
      421 ttcctttgcg acggaacacg cctgaccccg tagcctgaac gctccagcag gtcacagcac
      481 gcagccgttg ccggatgctc gatgttggat gtgacgatat tcgacctgcc gtggcctaaa
      541 gcgacggcac cgcgaattgc gagattgctc gcttccgtgg cgccgccggt gaagatgatc
      601 tcttcggggg cagcctcgat gaggctggcg acgcgtgccc gtgcttcctc aacggcatcg
      661 cgtgcccgtt tgcccagggg gtagctggat gacgggttgc cgaactcgcc ctgcaggaac
      721 ggcaacatcg cgtcgagcac ctccggatcg acgggcgttg tcgcgttata gtcgaggtcg
      781 atctgagcca cagcggagtc ccttcgaaaa aagccgggcg cagctgatcc gcgcccggcc
      841 gaattgaaag cagccgtcag gccgtcactt ccttggccgt gatcttgtag gtgaacgtat
      901 cgggatcgag gcagtcgagc ctgccctccg ccgtctcggc ctgcggatac atcaggaatt
      961 ccaaagttgg tcctttggcg cccggcaggt cgaggtcgtg cgcgacgttg taggcgagcc
     1021 agttgccctc ccacgagccg aacagagcct tccgcgcagc ggcaacttta tcgtccgaga
     1081 tggacagatt gccgggtggc tcctcgagga tcaccttgcg gacatcggcg ggatcgaccg
     1141 gcacccaacc gaagcctgag aggtagacct cggcccggca atgctgcgcc ttggtgacca
     1201 cctccgagcc tgcacccaag ctcttgtagc cgaatttcga tggagcgacg cgcaaaccat
     1261 aaatgtcacg agcggggagt cctgcagcgc gcgcgaggcc gacgtagagg gcattgaggt
     1321 cggcgcactt gccgcccagg ttcccgctct tcagcatcga ggcgatgtcg ccgatgccgc
     1381 agccccgcgt gttgggatca cgatgtgtat tctcgacgat ccattcgtag atgcggcgcg
     1441 ctttctccag atcgccgctc gcgccggcgg tgatcttgtc ggacgtttcc ttcacgatgc
     1501 cgtccgtggg gatgagctcg gttgccgacg tgtagagctc gcggtcagcg ccggagagcg
     1561 agggggcgcc gcctggcttg ctcaggtcga tggcgcggtc tcgggtcgag aacttgcttg
     1621 tcacctcgat ggcagccggc tcgccttcgg cccaaacgac atgtaggaat tccgcgccgt
     1681 atttggaatc gcgcttgatt tccgcggtct tggcgttggt cgtccaggtg ctgccgccgg
     1741 gacggaacca ttccgggtcg ctgaacgccg gcaggggcac ccaggcttgc accacgcccg
     1801 ccggcttgct gatttccagg cgcgtgactg tctcgaacgt gcgccacgcg ccgggtcttg
     1861 gagcgaacgt gctctgggct gatgccaagc cgggatacaa agtcgccgcg ccgagtactg
     1921 ccccggcccg caaaacgtcc cgtcgattta ccgccaccat catgtttcct ccggttttga
     1981 taggtgagaa tcagttggaa tggcatgcgc ccatgttctc gagcagagcc agtacgtccg
     2041 gtcgctgcca gtcgagatcc ccctcgacct gcaggcgaat agcgccggtc gggtcgacga
     2101 cgaaagtggt cggcagtaca ctcacgcccc atgccttcgt cgtactccgg tcggcatcga
     2161 ggagaaccgg gaattcgaca gggcttgtct cgaaatagcg tttcacgcgt gccggcggct
     2221 cggcgacgct caccgccaga accgcaaggc tccggccttt atcgcgcgcc aagagctttg
     2281 agagggaagc aagctctggt cggcatggct cgcaccaagt ggcaaagaaa tgtaccagga
     2341 tggtcttgcc ggctgattgc tcgagcttgt gccgtgatcc gtggatgtca tcaagcgtga
     2401 gcgcaggtgt ctccttgccc gctactgcct gcaattgggg gtgaggtgcg ctcgtcttcg
     2461 atacttcgaa atagaaacca cttgcgtccg caaggacagc agacacgctc gccacgagcg
     2521 tcagatacag caaccctgcg agctcaaaag aacgagcccg ccatggtgac ggtatgaaac
     2581 tgaagccccg acccgcggga gcagcgtggc tgcacatgaa gtgccgtaaa aaatacgtga
     2641 ttttgcaggg cagtcaagcg gtgaaggcgg ctgggcggcg tgttgaccgc tacgttccag
     2701 gtgctctggg gctttaatgc attgaagatg aatgataatt tgtagacttt gcataatttt
     2761 gcaacatcac attgacggag ttcacttttc tggcctaacc tgccgtggtc cctggtctct
     2821 acaggaggga gcactgatga acctcgaagt ctctcgccga cacttcatga agctggcagg
     2881 tgccggcgtc gcggggtcgg ctgtaaccgc tttcgggttt ggggaggcgg aggcacagct
     2941 cgctgcgcac gtccggccct acaagctcgc gcagacgaaa gaagcgcgca ctatctgtcc
     3001 ctattgcgca gttagctgcg ggatgatggt cttttcagcc cccaacaagg cgaacggcgg
     3061 caagctggag gtcacccaca tcgagggcga cgtcgaccat cctgtcaacc gcggaactct
     3121 ttgcccgaag ggctcggctg cgatcgacta catccgctcc aagtcgcggg tgaaataccc
     3181 gatgtatcgc aagcccggct ccaatacgtt cgagcgcgtg agctgggact tcgcgatgga
     3241 ccgcatcgcg agactgatga aggacgatcg tgacgcgaac ttcatcgaga ggaacaagga
     3301 tggcgtaatc gtcaatcgct ggacgacgac ggccttcctg tcgggaagct ccctcacgaa
     3361 tgaaggcggc tggctgacct acaaggtcgc tcgcggcctg ggaatgctcc agctcgaaaa
     3421 ccaggctagg atctgacacg gaccgacggt gtccagtttg ggcccaacgt tcggtcgcgg
     3481 tgcgatgacc aacagctgga ctgacatcaa gaatgctgat gtcatctgga ttatgggtgg
     3541 aaatgctgcg gaggcccacc cctgcggatt caaatgggta actgaggcga agcaccatcg
     3601 cggtgccaag ctgatggtgg tcgacccacg gttcacgcgg tcggctgctg ttgcggatca
     3661 ctacgcaccc attcgggccg gaagcgatat cgccttcttg tccggcgtgg tgaactatct
     3721 tctcaccaac aacaagatcc ataccgagta cgtacgccac tacacgaacg cgtcctatct
     3781 cgtccgggaa ggatacgcct tcaacgaagg cctgttctcc ggctacgacg aggacaagcg
     3841 ggcgtacgac cgcagcacgt gggaatacga gatcggtccg gacggctatg ccgtgcgcga
     3901 catgacgctc cagcatcctc gctgcgtctt ccagctgatg aagcagcact tcgcgccata
     3961 tacgcccgaa gtggtgtcgc gcatctctgg aacgccacag gataagttcc tgcgcgtttg
     4021 cgaatgggtg gcgtcgaccg ccaatggcga gcggtccatg accatcatgt acgcgctcgg
     4081 ctggacgcag cattcgcacg gtgctcagaa catccgcacc gccgcgatga ttcagctgct
     4141 gtgcggcaac atcggcatcc cgggcggagg tgtgaacgca ctgcgcggcc actccaacgt
     4201 ccagggcatc accgacgttg gcacactgac ggcagccatc cccggctacc tggccttgcc
     4261 gacggagaac gagcccacgc tcgagtcgca tctggccaag cgcaagttca cacccttgca
     4321 gccgaaccag acgagctatt ggcagaacta tccgaagttc ttcgtcagct tcctgaagtc
     4381 gttctacggt aacaaggcga caccagcgaa cagcttcggg tatgactggc tgccgaaact
     4441 ggacgtggcc tacgactgct tgcgcatgtt cgagaccatg ttccagggca agaccaacgg
     4501 cctgatctgc cagggcttca acccggtgat ggcaatcgct caccagaaaa agagcaccgc
     4561 tgggctatcg aagctgaaat tcctggtcag catggatccc atcgaaacgg acacggcgcg
     4621 gttctgggag aaccacggcg aggccaacga cgtggacccc tcgaagatcc agaccgaagt
     4681 gttcatgctt ccggtcacga cgttcgccga ggaggaggga agccttacca actccagccg
     4741 ctgcatccag tggaagtggc gggcggcgga tccgagctac gaggcaaagc cggacatagc
     4801 catcctggcc gacctgttca tgcggattcg caagctctat gaacaggagg gtggcaaagg
     4861 cgccgagcct cttctgaaca tcgcttggga ttacgccaac ccgctcgagc cgaccgctga
     4921 cgagctgctc aaagagctca acggcaaggc tctggcggac atcagagacg cggaaggcaa
     4981 catcatccgc agggcaggcg agcaactcgc cagcttcggc gagatgcggg acgacgggtc
     5041 gaccgacggc tccatgtgga tctacacagg agtctacggt cccaacggga acaactctca
     5101 acgccgggat aatagcgatc cttcggggtt gggcgtctac ccgaattggg gcttcagctg
     5161 gcctgccaat cgccgcatcc agtacaaccg cgcttcagcg gacccccaag ggagaccttg
     5221 gagcgaggcg aagaagtaca tgtactggga cggcagtcgt tggaccggtc cggacgtccc
     5281 ggactatgtg ccgacgatcc cgccggagcg ggccacgggt ccgttcatca tgaaccccga
     5341 aggcgtttcc cgcctgtggg ttcgtggact gatggccgac ggtccgttcc cggtgcacta
     5401 cgagcctttc gagtctccgg tcgaaaatcc cttgttcccc aagatcaagg ggaatccggc
     5461 ggcgcgggtg ttcgcgaccg atttggacgt cttcggcgat ccgaaggagt tcccgattgt
     5521 cgcgacgacc tatcggctca cggagcactt ccactactgg acgaagatgg tccacatgaa
     5581 cgcggtcatg cagcccgagt tcttcgtgga aatgtcggag gagcttgcga gagagaaagg
     5641 catcaaaaaa ggagacatgg tcagagtctg gtccaaccgc ggctcggtca aaggcaaggc
     5701 cgtcgtgacc aagcggctca agccgctgac gatcaacggc aaggtcgtgc actcggttgg
     5761 attgccactg aacttcggct ttatcggcga gaccaaaaag gtcgctccga taaactcgct
     5821 gacgccgcct gtcggcgacg ccaacgcaca gacaccggag ttcaaggcgt tccttgttga
     5881 tatcgagccc gtccgcgggc cggtggcata gggaggtcga gagccatgta tcctccaatc
     5941 gcaaatccca ccaccgagcc aactcccccg aaattcgggg agaacgacct gatacggcgg
     6001 tcggcgtcca cagtgacgcc gccagcccag cgccagaccg aggtggccaa gctggtcgat
     6061 gtttcaaaat gcatcggctg caaggcctgc cagacggcgt gcctcgagtg gaacgagctc
     6121 gaggaagagg tcggtgtgaa tgtgggggtt tacgacaatc cccacgacct cacagcgaac
     6181 acctggacgc tgatgcggta caccgagtgg gagaacccgg aaaccaacaa tcttgagtgg
     6241 ctcatccgca aggatggctg catgcattgc gaggatccgg gttgtctcaa ggcctgcccg
     6301 tcgcctggcg cgatcgtgca gtactcgaat gggatcgtcg atttcattca ggagaactgc
     6361 attggctgcg gctattgcat aaagggctgc cccttcaaca tcccgcgcat ttcaccggtc
     6421 gacaacaagg cctacaaatg cacgctctgt tcggatcgcg ttgcggtggg gcagggtcct
     6481 gcatgccaga aggcttgccc gactcaggcg atcgtattcg gcccgaagga tgaaatgaag
     6541 aagtgggccg atgagcggat caaggacctg aaatcgcgcg ggttcgagaa tgcaggcctt
     6601 tatgatccgc ctggagttgg cggtacccac gtgatgtacg tcctgcaaca cgcggataag
     6661 ccggagctct atgcgggatt gcccaacaac ccgaagatca gcccggttgt cgaggcgtgg
     6721 aagggcttga gcaagtatgc cggaatggcc gttctcggcc tgacggcggc tgcaggcttc
     6781 gtccactaca ttctgcaggg cccgaatcgc gtaaccgacg aggacgagca gaacgcggac
     6841 aggcttgctg gaggaaattc acaatgagtc aggcaaaaac ttcggcgcac cttcgggtgc
     6901 gccgttactc cggaagtgcc cgcatcaatc actggatcgt ggcgatctgc ttcgtcctgc
     6961 tgatgctgag cgggctttcg ctgttccatc catcattcta tccgctgagc gccctgttcg
     7021 gcgggttgca aatcgcgcga gagattcatc cgtggatcgg actagtgctg gtcgtggcct
     7081 tcctggggct gttcatccgc tttttccccc agaacttgcc tgagacgacc gatttcgtct
     7141 ggctcgcacg tctccgccac gtgctgacgg ccaacgataa atttctgccg gaggtcggca
     7201 aatataatgc tgggcagaag tttgtcttct ggtcgcagtt cgtgctcatc ggcgtcatgc
     7261 tggtgaccgg catcgggctc tggagtgagg ggcgcgccta cgtcgaggag cttttcggct
     7321 ttacgacgac catcgagcaa cagcggtggg cggcactgct tcattcgtcg gcggccgttc
     7381 tcgccatctc cgtctggatc gtgcacgtct acgcggcgat ctgggtgcgc ggcaccatca
     7441 gcggcatgac gcgtggcacg gtgaccggcg gctggggttg gcgccatcac cgcaagtggc
     7501 tgcgcaagga agttgaaagt ggcggggcca aaaaggttgc ctagccaaag ttggcgggca
     7561 gttaccagcc tctcgtagaa ctcatcctcg gttcggggtg cttatgaccg aaactggctc
     7621 agccaaattg catagcgtca acatcgggga ggaggcgaag ccgccggccg cggtcctccc
     7681 ctatcccgcc tctctcttcc tcaaccggtc gaagcggctc ctcgcacttg ctccagggca
     7741 ccaactcgaa ccctatctgc gttttctttc tgtcgtaacg catgcccagc acgacatcca
     7801 ggcggcactt cccgacgctt cagttccacc gcccgaacag atcgcgcagg ctatcgagca
     7861 tgggatgccg cctatctcgc gcttcacgct caagcctgac gatgtgagtc agacgacagt
     7921 cgatcacctt ctagaaaatc tcgcgcagag acagctatcg gccgaggctg cggctgcggt
     7981 acaaacttgg cggagcatga ccggcgagca gcgtcgagcc gccatggcgg atgcattcag
     8041 cgaggcggat tcgcaggaca gcatcgcttt tcgcgccctc gttctggccg gactccaagt
     8101 gcacttcacg cgcatggcgg cgaagctcga tgcggccagt ttgaagccta tcgcggatgg
     8161 cgcttgtccg gcgtgcggaa gcccgccggt ggcgagctcc gtggtcaact ggccgggtgc
     8221 aaccaacacg cgcttctgca cgtgctcgct gtgcgcgacg atgtggtacg tcgtgcgggt
     8281 caaatgcctg ctgtgcagct cgaccgaaag catcagctac cgcctgatcg acggctatcc
     8341 ggatacgatc aaggccgaaa cctgcgacaa atgccacgga tacgtgaaga tcttctatca
     8401 ggtcaaagat cctgttttgg aggcgctggc tgacgatgtc gcctccctcg gcctcgatat
     8461 gctgcttacc aaagagggtt ggaagcgggg tggccacaat ccgtttctga tcggctactg
     8521 agatcatgcg ggcctgtccg ggatcgctgc ttgagcgtag gccatgaacc aggcgctccc
     8581 catatcgccg acgcgtcttc cgtcagtcga acgggtgctg cggacagatg tgggtgcggc
     8641 cgcgatcgca cgtttcggac atgaagcgac tgtcgacgcc gtacgtcgcg ttcttgatca
     8701 ggttcgtcag gccaggggct cagccatcat cgccctgccg gacaaacgag ccgttgcggc
     8761 cgaggcattg gagctgcttg agcgtgaaca tgctccgagc gttcgccgcg tattcaatct
     8821 ttcaggaatc gtactgcaca caaatctcgg gcgagcagtc ctccccgaag ctgcgatcga
     8881 agccgcgatc atggctatgc ggcagccggt tgcgctcgag ttcgacgtcg gcagcggcaa
     8941 acgcggcgag cgcgatgatc acgtgcgcgg gctcatctgc gagctgaccg gcgcagagga
     9001 tgccgcgatc gtcaacaaca acgccggcgc cgttcttctg gtgctcaaca cacttgccgc
     9061 aggcaatgac gcgatcgtct cgcgcggcga gttgatcgag atcggcggct cgttccgtct
     9121 tccggacatc atggcacgcg ctggggcgcg tctcgtcgag gttgggacga ccaaccgaac
     9181 acatctgcgc gattatgagc aggccatcgg ccaacatacc ggtctgatcc tcaaagcgca
     9241 tacgtcgaat tacctgatcc aggggttcac caaggctgtt ccggcgtcgg agctcgccgc
     9301 tctggctcgg cggcacaatc ttccgttcgt caatgatcta ggatcaggtg cgctcgtcga
     9361 tctcagtcgt tacggcctca ggcccgagcc aaccgtgcgg gaaaccctct ccgagggcgc
     9421 ggatctcgtc actttctccg gtgataagct gctcggcggt ccgcaagcgg gtattatcgc
     9481 cggccgcaag gatttgctcg agcgcgtccg caagaacccg atgaagcggg cactacggat
     9541 cgacaaaatt cgtttggcgg ccttggaggc tgttctcaag ctctaccgcg atcccggccg
     9601 cctccatgaa acattgccga ccctcaagtc tttcgttcgg ccccaaaagg atatcgaggt
     9661 cgcggcaacg cggctgctcg gcccgatttc tgcggccgtt ggatcgacat acgatgtcgg
     9721 catcatcgaa tgcagcagcc agatcggctc cggcgcgctt cctctggaaa ccctgccgag
     9781 cgcagggatt gcgatgactc cagccgaccg caaaggtgca gggacccgcc tcgaggcgct
     9841 tgccgcagct tttcgggccc tgcccatccc agtgatcgga catatcagaa agggtgcttt
     9901 cgtaatcgat atccgctgtc tggaggatga ggccgctttt cttgcgcagc ttgatcagct
     9961 gaagaacagc atcggcaagg gagtacgctg cgatgctgca ggctgactct cggcgagatg
    10021 agcgagatga gacggacagg cgcgctgagc gtatggccca cgccgccgag gcggcagctc
    10081 gcggcgatta cgccacagcg caaggcatct ggatggtcta tgcgcaagct ggctatgcca
    10141 gggctcaagc cgaaatgggg caatgcttcc tgcacggctg gggcgtcgaa cgcgatcccg
    10201 atctggcctg cttttggctc actctagcgg cgaacgccgg cgatcctgtc agtcagagct
    10261 tgctcgggga tttctatttc aacagcgagg cgggccggcc gcagcctgcg attgccgaaa
    10321 aatggtacgt ccgcgcggcg aagcagggcg acccgcacgc gcaggacatg ctgtcccgga
    10381 tcctgatgga aggccgccac cgtaagccgg attacaagca ggcgcacgaa tgggcgttga
    10441 aggccgccga gcaaggcgtt gcggccgcca tgacacgcct gggccttctc tataaccagg
    10501 cgttgggcgt cgagcgcgat ccgaaaagcg ccgccgaatg gtggcgaaaa gcggcgatca
    10561 acggcgatgc cgatgggcag gctatgctcg gcgcggccta tcacttaggc gctggtatcg
    10621 agcgagaccg cgttgcggcg ctggcttggc tgacacgggc gcgccgtgca cgcagcaagc
    10681 ttgcggaccc gtactatcca gttgtgcgcc agagctgcag ccccgatgag cggcgtgagg
    10741 cagagcgtct tgcgagctta cccctcggcg ttgaggcaag atcatgatcg tcggcaccgc
    10801 tggacatatt gatcatggga agtcgtccct cgtgatgcgg ctgaccggtg tcgatcccag
    10861 ccgcctgaag gaggaaaagg ctcgcggcat cacgatcgat ctggggttcg cctattggcc
    10921 tcggccgggc ggcgagatca tcggtttcgt tgacgtcccg gggcacgagc gtctcgtgca
    10981 caacatgctc gccggagcca cgggcatcga tttcgtgctt ctcgtcatag cggccgatga
    11041 cggagtgatg ccgcaaacgc gcgagcactt ggcgattatg gatctcctcg ggctcgagca
    11101 tggcctcgtc gccctcaaca aatgcgacct cgtcaacgcg gaacgactgg ctgccgtcac
    11161 ccgagatgtc gagaacgttc tagcgggaac ggcgcttgag ggttgcgaaa tcctgcctgt
    11221 ttccgccgag accggtgcag gcgtggatgt gcttgccgcg cgcctcgatg cggcgcttgg
    11281 tcgagtcgca tcacgtccca agtcgggacg gttccggctt gcaatcgacc gttcgttcac
    11341 gctggccggg ctcggcacgg cggtcactgg aactgtgctt tcaggcaccg tgcacatcga
    11401 cgatcgcgtg gtcgtgagcc cttcgggcat cgaagcgcgt gtgcgctcga ttcacgccca
    11461 aaacaagccg gttgagcgcg gcgaagccgg tcaaaggtgt gcgctggttc tgagcgggcc
    11521 ccgcgtgagc aaggagaccg tgaaccgcgc ggatgtggtc ctggatccca ccctgcacgc
    11581 accaactgct cgtatcgacg ccaccctccg tgtgctgccg tctgagtcga agccgattac
    11641 acagtggttc cccgtgaagc tgcatcacgc cgcagccgaa gcgcccggca ggctggtttt
    11701 gctccgcgat gagccaatcg cgccaggaga aactgctttc gtgcagctcg tgttggaacg
    11761 cccaattgcg gccgcggcgg gcgaccgttt cgtcattcgt gatacgactt cgagtcgcac
    11821 actcggcggc ggcgtcctcg tcgatctccg cgcgcctgag cggcgccggc gaacgccgga
    11881 acggcgcgca accctgacag gtctcgccga aaaagagcct gccgcggcgc tccggcgcgt
    11941 gttggcggtg ccgggaagct ggatcgatct tgacgcattc ttccgcgacc gggcgatggg
    12001 cgaggctgaa gctgctgcag tcattacgga gctgtctctc gttaccctcc cgtttgggag
    12061 cagccgtgcc gcaatgctac cggacagctg ggagcgcttg agagtgcgca tcggcgagac
    12121 gctcgatgac ttccacgcca agcatcccga ccggcccggt attgggctcg agcagctgcg
    12181 tttggcggga gacaaaccac cgcttccggc tccgctcttt acgtcggcat tgagaaagct
    12241 catcgaagcg ggagaggttt cgctcgatcg cacgtgggta cgccgccccg gccatgaggt
    12301 gagattttcg ccggaagagg aggaaatctg ggaaaacata aaacccctct tggcgcagga
    12361 gccttaccgt ccgccacgcg tccgcgacat cgccaacgac ctgtgcatcc cggaagcttt
    12421 cgtccgtcgg ctgatgcata tggcagcacg gcgcggtgat gtggacgaga ttgcacacga
    12481 ccactttttc ctgcgtcaga ccgttcaaaa gatggccgag atcgcgatcg acgtggcagc
    12541 ccaatcaccg accggcaaat tcaccgctgc cgatttccgc gatcgcttga acaacggccg
    12601 caaggtggct attcaaattc tggaattctt cgatcggcat ggattaacga tccgccgtca
    12661 ggatctgcgc cgcatcaacc cggcgcgcat cgagttcttt gcgccgaagg cgcaattccc
    12721 cgatggagga gtcccgctcc cggtggggcg accggacttc aaatccgggt ggggccgtca
    12781 gacggtctca ggtgggttcg actcccatcc tcctccgcca acaaccagtg agatgtcacg
    12841 atgatcccac ctgttcaagc cttcctggct gaattggaaa gcgccgcaat ctcagcgaac
    12901 caagccgaag gcgagcttcg caaacgaatg gcagaagaga tcacacgtct cgagcgccag
    12961 cgcgcttttg cttttcgccg gctcaatttg atgcgcgagg tggccaaagc catcgcctcc
    13021 gccgagaatg aggaatcggc cattgcctgc ggacttgcag tggtgcgtac cgagctcggc
    13081 tgggaaacag aaaacgaatc acgcaccgag acgctgaacc acttcgcacc ggtcgttcgg
    13141 gccgcctttg aagggctttg tccgcctgag acgggcgagg ccacaggcaa tgtcgccgag
    13201 gcactttccg gtttcgagga ttggtacgaa tccgcaaatg ggaaaccgtt ctgggtgctc
    13261 tttgatcagt acgtcccgga attgcctctc gtcgagcggt gttaatcggc catgccgcgc
    13321 ggtatcaaac ggaaacgggg tggcaataac gcagcggatt gtgcgcgatc gtcctgaaga
    13381 aatgaaaaag aaactgctca tcgttctcgt caacacggac ccgcgcaatc cggaggagct
    13441 ggccgcgccg ttttaccacg cggcagtggc tgcagcgatg gatatcgagg tcgatgtcgt
    13501 gtgcgcggcg acggccggca agcttatgcg gaaaggcgtt gccgaggcga tccgcgtcaa
    13561 gccaacgcat gacaagaccg tctacgactg gatttgcgac gcacacagaa caggggctcg
    13621 gttctgggca tgcccggcca atcttgatct cttccatatg acggaggaag atctcattcc
    13681 ggaatgcaag ggtctgatgg gcgcagctac catgcttgag aacatgatgt ctgaggattg
    13741 ccgcgtcctg agctactgag gcggccgatg gtgctgatca acggcaagga atatccggag
    13801 catttctact acgatctcga cgatcagatc tggtacgagc cactcgaaga tgggaccgtt
    13861 cgtgtcggct tcacgcctgc cgcaatggat ttggcaggcg aaattttcgt ttttacgcct
    13921 aagcgcaatg gttaccgatt cgaggcgcaa agatcatttg ccattataga gggtggcaaa
    13981 tgggtgggcg ccgcgcatgc cgctttcgct ggcgtcatcg tgaacgccaa ctccgagctg
    14041 caacgccgtc ccgcgttgct caatgaggac cccttcggcg caggctggat gctgaccatg
    14101 cggcctgagg acgaaaattg gcgtgatcgc ctcctcaccg ggccagagat cggtccggcc
    14161 ttctcggcat ggattgccgg aagccgacac acctaaccat agcgggcgtg cggcggcgaa
    14221 atcgccaagt tggccggaac gaattcgcaa accggacatc gaaccggccc gcggatctac
    14281 gaagagagct ggaaaggcct ggggacatca gctggctggg ggacgaggat tcgaacctcg
    14341 actaacggag tcagagtccg ctgttctacc attaaactat cccccagcac ggggctggcc
    14401 ttggcaggtc gccatgggcc gaggagatgg ccgtatatca gcttgtcttt ggaactgcaa
    14461 ggcaggtgcg cgcacttggc aacggccgcc ggacaagcgc ttcaggggcg gaatggaccg
    14521 ttcgccgtcc cgccaccctg gaagccgaaa ccgacttcag cccagcatgt ttgtgtcgcc
    14581 acacaccgag atcgcctggc ccgagatcgt acggccgcga gggcttgccg tgaagacgac
    14641 catgtcggcg agctgctgtg gggtgacgta ctccttcagc gaggcgtact gcatcatgtg
    14701 ctcctcctgc tccttgaagg agacgccgcg ctgctgggcc ttggcctcga tgacccgccg
    14761 aatgcggtcg cccgcgacga ggccgggcag cagggcgttg gcgcggatac caaaaggccc
    14821 gagctccatg gcgatcgtct tcgtgaagcc gaccacgccc cacttcgccg cagcgtaagc
    14881 cgagcgcagc ggaaagccat gctttccggc ctgcgaggag aggttcatga tgctggcgtt
    14941 cttgctctgc ttcaggtgag gcaccgccag ccggacgcag ttgaactgtc ccgtgatgtc
    15001 cactgcgagg cagcggtccc agtcctccgg gttgatctcc tcgacccggc cggtcgggcc
    15061 tgcgatcccg gcattgttga cgaggcagtc gaggccgccg agctgctcga gggcttcctt
    15121 gaacatggct gccacctggt cacggtcagc gacatcgcac acgctccagg tcagcttcgg
    15181 gtccgtcttg gccagggcct cgagcgccgc cttgtcgata tcgcacacgt gcaccctggc
    15241 cccctcccgc acgaatgcgc gggcaatctc gagaccaata cctgcggctc cggcggtaac
    15301 cagtatgcgc agaccttcga tctgcatatc catgatgctc tctcccggct ctgatgttat
    15361 caggtttcca ttatcccatg ccgcaactcg cttaaagcga ttgggaccga gagaagcgga
    15421 cacgaaacaa cgggaggaga atggcatgcc gaaggtggcc atactcgggg cagggctgat
    15481 cggccgcgcc tggtcgatgg tattcgcacg cgccggctgg gacgtgacag tgtgggacgg
    15541 ggtcgcgggc gtcgcggaga atgcgctgag cctgatcgcg cagggtctcc aggacctgag
    15601 gcggcatgag ctgatcgatg atcccgaggc ggccttagcc cgcgtcagcg cagccggcga
    15661 tctcgaaaat gccgtcaccg gggctgatct cgtccaggag agcacgcctg aaacgctgga
    15721 gatgaagcgg gagattttcg ccaggctcga ttctgcggcc ccgcctgagg ctattctcgc
    15781 atcctcgacg tcctcgctcg tcacttcgct ttatacggag gatcttgccg gccggagccg
    15841 ttgcctcgtg gcccatcccg tcaatccgcc gcatctcgtg ccgatcgtcg agctatgcgg
    15901 tgctccgtgg acgtcgcctg aggtgatgca acgcgcacga gagatctatc gctcggtcgg
    15961 tcaggtgccg attcatgtca agcgcgaggt ggatggcttt gtgctgaacc ggctgcagac
    16021 ggctctcctc gccgaatcgt tccggctggt ggaggagggc gtcgtcaccc cgcaggacct
    16081 tgatgaaacg attcgaaacg gactgggcct gcgctgggca ttcatgggcc cgttcgaaac
    16141 gatcgagctc aacgctcccg gcggcattcc cgactactgc cggcggtaca gcccgttttt
    16201 ccgccgcctc gccgacgatc agccgaaagc gtccgtatgg gacgaccaca ggacggctga
    16261 ggccgctgcg gcttggggac cgatccccga tccgcagaag cacgccgcca agacgcggtg
    16321 gcgagacgaa cagcttgccg cactcgcggc tcacaaacgc agaatgaaac ccttcactga
    16381 ctgagaggcc ggacccatga gcaaatcccg caaggtcatc atcacgtgcg ccgtcaccgg
    16441 ctcgatccac acgccgtcga tgtcgccgta tctgccgatc acgccggagg agatcgcgga
    16501 tgccgccgtc ggcgcggccg aggcgggcgc cgctatcgtt cacctgcacg cacgggaccc
    16561 gcagacgggc aagcccgacc agagtgtcga ggcgttcgcg cc
//
LOCUS       DASVDU010000021         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_154094, whole genome shotgun sequence.
ACCESSION   DASVDU010000021 DASVDU010000000
VERSION     DASVDU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154094"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VF226_01665"
     CDS             complement(<1..365)
                     /locus_tag="VF226_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEX7072721.1"
                     /translation="MTGQDVVATIEQPGSTEVAPRQAITPMEMLQIAVEKGATLDQLQ
                     KLMDLQERWEANEARKAFVAALSAFKAHPPMVRKNRRAGFESRRTGDKTEYEYATLDH
                     VVAAITPALSANGLSHRWET"
     gene            complement(376..501)
                     /locus_tag="VF226_01670"
     CDS             complement(376..501)
                     /locus_tag="VF226_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072722.1"
                     /translation="MLHWFMNFFRRRPEYRRAPPLKNAHYIGLHIANAGGRSALR"
     gene            complement(655..1029)
                     /locus_tag="VF226_01675"
     CDS             complement(655..1029)
                     /locus_tag="VF226_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072723.1"
                     /translation="MTSPYLLRPTRTHSQVALERLRTKLDEALAACIAAEADEIKGYI
                     LDAIGDTGLLIREQEEADEPCTDEAYARGCTCRMESVYATMIDPPEPIIDRFCPLHGR
                     DDPDRRYDEMRDERVTLFDEAS"
     gene            complement(1035..1289)
                     /locus_tag="VF226_01680"
     CDS             complement(1035..1289)
                     /locus_tag="VF226_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072724.1"
                     /translation="MLLTEEESRERWCPHVRAMNFAPAIDHNPAVNRPTADYHCIGSA
                     CMAWRWACGPRNYTHAAESERRKYPPNGRGYCGLAGRPEE"
     gene            complement(1293..1625)
                     /locus_tag="VF226_01685"
     CDS             complement(1293..1625)
                     /locus_tag="VF226_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072725.1"
                     /translation="MLNTAKLTAAVETLRAYCEQVRLAMNARGYAGAEIHLTVRSGCP
                     GYYGPGSIGAYDDGQMGDGIFQHFALTEFEKALAYAEARPRRATEADIAATLGITPDG
                     RVLEVVEG"
     gene            complement(1632..2330)
                     /locus_tag="VF226_01690"
     CDS             complement(1632..2330)
                     /locus_tag="VF226_01690"
                     /inference="COORDINATES: protein motif:HMM:NF014786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072726.1"
                     /translation="MAQGFDQKRRVRISADTLAAVAPFICREEVRYYLNGVYVEPHRD
                     GGVIVTATDGHTLATCRDENGSADRDWICSVPPVIRSAALRGGKGKSAPKTVHFIGSA
                     AHVLSEVDDSYDASTPGPMHLATAYAPAIDGTFPDWRRAAPGVVTHLDGAGFSLNPHY
                     FTRFAEAGKALGAHGVTLLLGKTPNDPIGVRVGNCQRFFGLVMPMRFVSDEQPVGMPL
                     LPEWCRPLQQAAAE"
     gene            complement(2599..2928)
                     /locus_tag="VF226_01695"
     CDS             complement(2599..2928)
                     /locus_tag="VF226_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7072727.1"
                     /translation="MDWLALYANLGGMPNSIRHWRKRRGLTLEQLAPRVGMTAGNLSR
                     MERGEIPYTSVALEALSKALGCKIVDLVIESDSPDLVVEATDELRHLTEEQRQQAVRL
                     IRALRAA"
     gene            2987..3187
                     /locus_tag="VF226_01700"
     CDS             2987..3187
                     /locus_tag="VF226_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7072728.1"
                     /translation="MTLSEYLKTKGIKLTEFAAKSGVSASTLSRIARGLHRPDWPTID
                     AIEKATGGKVGPSDYRLERAES"
BASE COUNT          565 a         1080 c         1114 g          608 t
ORIGIN      
        1 gtttcccagc ggtgggacaa cccattggcg ctgagcgccg gcgtgatggc cgcgaccacg
       61 tgatcgaggg tggcatactc gtactcggtc ttgtcgcctg tccggcggct ctcgaagccc
      121 gctcgcctgt tcttcctcac catcggcggg tgcgccttga aggcgctcag ggcggcgacg
      181 aacgccttgc gggcctcgtt cgcttcccag cgctcctgga ggtccatgag cttctggagc
      241 tgatccaggg tcgcgccctt ctcgacggcg atctggagca tctccatcgg cgtgatggcc
      301 tgccgtggcg cgacctccgt cgatcccggc tgctcgatcg tggcgactac gtcctgtccg
      361 gtcatgtgca ttctcctagc gcagcgcgct ccgtccgccc gcattggcaa tatgcagccc
      421 gatgtaatgg gcgttcttga gcggcggggc gcggcggtac tccggccggc ggcggaagaa
      481 attcataaac cagtgcagca tgtatccatc tcctctgagt taccgggcgg cccggttacg
      541 accgccgggc cggctggggc ggatctcaca tgccggctgc gacgaagatc aggaacagca
      601 cggccagcag aaacgagaac gcgaggccgt tgaggattcc cttgaccgct tccatcacga
      661 cgcctcatcg aacagggtca cgcgctcgtc gcgcatctcg tcatatctcc ggtcgggatc
      721 gtcccggccg tgcagcgggc agaaccgatc aatgatcggt tcgggcggat cgatcatcgt
      781 ggcgtagacg gattccatcc ggcaggtgca gccgcgcgca taggcctcgt ccgtgcaggg
      841 ctcgtcggcc tcctcctgct cgcggatcag caggccggta tcaccgatgg cgtcgaggat
      901 gtaccccttg atctcgtccg cctcggctgc gatgcaggcg gccagggcct catcgagctt
      961 cgtgcgcagg cgctccaaag cgacctggga atgggtgcgg gtggggcgca gcagatacgg
     1021 cgatgtcatg gccgctactc ctccggtcgt ccggcgaggc cgcagtagcc gcgcccattg
     1081 ggcggatatt tccggcgctc agactcggcg gcatgcgtgt agttgcgcgg cccgcacgcc
     1141 catcgccacg ccatgcaggc agagccgatg cagtgatagt cagcggtcgg ccggttgacg
     1201 gcagggttgt gatctatggc cggtgcgaag ttcatcgcgc ggacatgcgg gcaccacctc
     1261 tcgcgagact cttcctcggt cagcagcatg tctcagccct ccaccacttc cagcacccgc
     1321 ccgtccggcg tgatgccgag cgtggcggcg atgtcggctt cggtcgcgcg gcgcgggcgg
     1381 gcctccgcat aggcgagggc cttctcgaat tcggtgaggg cgaaatgctg gaagatgccg
     1441 tcacccatct gcccgtcgtc ataagctccg atcgagccgg ggccgtagta gccggggcag
     1501 ccgcttcgga cggtcaggtg aatttctgcg ccggcatacc cccgcgcatt catggcgagc
     1561 cggacctgct cgcagtacgc tcgcagcgtc tcgacggcgg cggtgagctt ggcggtgttg
     1621 agcatggcgc gttactccgc tgcggcctgc tgaagcggcc ggcaccactc gggcagcagc
     1681 ggcatgccca ccggctgctc gtcggaaaca aatctcatgg gcatgacgag cccgaagaac
     1741 cgctggcaat taccgacgcg gacgccgatg gggtcgttag gggtcttgcc gagcagaagc
     1801 gtcacgccat gtgccccgag cgcctttccg gcctcggcaa accgcgtgaa gtagtgcggg
     1861 ttcaggctga agccggcacc gtcgagatgg gtgacgacgc ccggagcggc ccggcgccaa
     1921 tcggggaacg tgccgtcgat ggcgggcgca tacgccgtgg cgaggtgcat tggaccgggc
     1981 gtcgacgcgt cataactgtc gtccacctcc gacagcacat gagcggcgga gccgatgaag
     2041 tgaacagtct tgggagcgga ctttcccttg ccgccccgga gcgcggcaga acgaatgacg
     2101 ggcgggacag agcagatcca gtctcggtcg gcgctgccat tctcgtcgcg gcaggttgcc
     2161 agcgtgtggc cgtccgtcgc cgtgacgatc acgccgccat cgcggtgtgg ctcgacgtag
     2221 acgccgttca ggtagtaccg gacctcttcg cggcagatga acggcgcgac cgccgccagg
     2281 gtgtcggcgg aaatgcgaac gcggcgcttt tggtcgaagc cttgggccat tcctctctcc
     2341 cttcacgacc ggcccggact acctgcccgg accggcctgg ggcggcttgt tgcacccggg
     2401 aggggtcacc ccgggcggcc agcgggtgct gacggggaga tatttgcaca ccctgcaaaa
     2461 tcagtccagc agaaattttg cgcgagatgc aaaatgttgg tcatgatgca aggccacccg
     2521 tgccgcagcg cagaatgcac cgacagtcag accagctcac ttgcctgatt ttattggggc
     2581 gcggtggtta acgcggcttt atgcagcgcg caatgcgcgg atcagccgga cggcctgctg
     2641 gcgttgttct tccgttagat ggcgcagctc gtccgtcgcc tcgaccacca ggtccggaga
     2701 gtcggattca ataacgagat caacgatctt gcagccaagc gccttggaca aagcctcgag
     2761 cgcaacgctg gtgtagggga tctcgccccg ctccatgcgg ctcagattgc cggcggtcat
     2821 acccactcgg ggggccagtt gttccagagt gaggccccgg cgcttccgcc aatgtcgaat
     2881 ggaattcggc atacccccaa gatttgcgta caacgcaagc cagtccatgc cgtctggtgc
     2941 aaattagggc ttgcactctt tttgcaggtc atgcaaactg gtccgcatga cgctctccga
     3001 gtacctgaaa accaagggca tcaaactgac cgagttcgcg gcgaagtccg gcgtctcggc
     3061 gtccacgctg agccggatcg cgcgaggtct gcaccggccc gactggccaa ccatcgatgc
     3121 gattgagaag gcgacgggcg gcaaggtcgg cccgtccgac tatcggctgg agcgcgccga
     3181 gtcatgactc cgacagcacc ctgccgagca gatttcttcg aggcggcctg accgtgcccg
     3241 tgtacttcat ccaggcgggc gagggcgggc cggtgaagat cggttatgcc gataacgtcg
     3301 ccaaccgcct tcgcaccctt cagacggctc attacgaagg gcttcacctg atccggacgg
     3361 ttgacgg
//
LOCUS       DASVDU010000022         8041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_739837, whole genome shotgun sequence.
ACCESSION   DASVDU010000022 DASVDU010000000
VERSION     DASVDU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8041
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739837"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1076
                     /gene="kdsA"
                     /locus_tag="VF226_01705"
     CDS             210..1076
                     /gene="kdsA"
                     /locus_tag="VF226_01705"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7072729.1"
                     /translation="MDSPTPDPAVSVGSVTFANDRQIALFAGPCQMESRAHALEMASA
                     LKEICARLNIGLVYKTSFDKANRTSLSGKRGMGLEAALPVFAEIRETLGLPCVTDVHE
                     AAQCGPVAEVVDVLQIPAFLCRQTDLLVAAAKTGRVVKVKKGQFLAPWDMKNVVAKIT
                     GSGNTRVLVTERGVSFGYNTLVVDMRALPIMAETGAPVIFDATHSVQQPGGQGTASGG
                     DRRFVPVLARAAVAVGVAGLFIETHQDPDRAPSDGPNMVPLNRLESLLAELIELDHVV
                     KKQLASARDGAA"
     gene            1209..2108
                     /locus_tag="VF226_01710"
     CDS             1209..2108
                     /locus_tag="VF226_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7072730.1"
                     /translation="MKRIICIGHSALDYIYRVEAFPRGPSKIRALEHFESGGGMAANA
                     AATIARLGGKVELWSRVGDDEAAAKIRAGLKSFGVDVRSVQSFEEGRTSTSAIIVDDA
                     GERLIVGARDINMPSGTSWLPLERISGAAAVLGDVRWLEGLRVVFERARKEGIPTVLD
                     ADLGAREALAEILALTDYAIFSEPALKDFAALPSTEAMLQRVRELGPRHAGVTLGARG
                     YRWVDASGGGEQPGFPVDVVDTTGAGDAFHGAFTLALVEGKPEAECARFACAVAAMKC
                     TRIGSRAGLPTRAQVEGFLAARS"
     gene            2448..4466
                     /gene="tkt"
                     /locus_tag="VF226_01715"
     CDS             2448..4466
                     /gene="tkt"
                     /locus_tag="VF226_01715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7072731.1"
                     /translation="MPLEQHRAAGSAEVSHKDMANAIRALAMDAVEKAKSGHPGMPMG
                     MADVATVLFTKFLRFDAADPKWPNRDRFVLSAGHGSMLLYALLYLTGYPGMDIEQIKQ
                     FRQLGSKTAGHPEYGHAAGIETTTGPLGQGIATAVGMAIAERIWNARLGPDVIDHHTY
                     VISGDGCLMEGISQEAITLAGHLGLGRLIVLFDDNGISIDGPTALSTSDDQIRRFEAA
                     GWNTASIDGHDPEAIASAISRARSDDSKPWLIACKTVIGYGAPKKQGTAATHGSPLGA
                     DEIAAARERLGWPYPPFEIPEPILNAWRSAGKRGAAERANWQERWAKVDAKLRQSFEH
                     PAAAAGEELRKAIGEAKRKAAAETAKKATRQWSELTLDHLVPVLPGLIGGSADLTGSN
                     NTKTKSQAVIRKGDFSGSYIHYGIREHGMAAAMNGLALYGGLIPYGGTFLVFSDYCRP
                     AIRLSALMRQRVVYVMTHDSIGLGEDGPTHQPIEHLASLRAMPNLNVLRPADGVETAE
                     CWEIAVGPGDTPSILALTRQAVPNLRTTHTDENLCAKGAYVLREPAGTRDVTLLATGS
                     EVGLAVEAAEALAKEGVAAAVVSMPCFELFRKQPEDYRRAVLGEAPRIGIEAAVAQGW
                     CEWLDRGDVFIGLSDFGASAPAAALFQHFGLTPGRVVEAAKALIAKRG"
     gene            4494..5696
                     /locus_tag="VF226_01720"
     CDS             4494..5696
                     /locus_tag="VF226_01720"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7072732.1"
                     /translation="MDLEKLKTTDGIDVHGKRVLVRADLNVPVHGGEVTDVTRLERLV
                     PGLLDLANRGARVVVISHFGRPKGSPSPEFSLKPVAAKLGELLGKPVAFVEDCVGPKA
                     QAAVNALAPGQFAVLENLRFHKGEEKNDPEFAKELASLGDIYVDDAFSSAHRAHASTE
                     GITHHLPSYAGPLMMAEIEALRSALDHPDPPTAAIVGGAKVSTKIPVLMHLIEKVDKL
                     IIAGGMANTFYLAQGVDVGRSLAEPDFRDTALDVMKAAKARSCEVILPPDAVVAAELK
                     EGVPTQVVSILDVPSDAMILDVGPKSVEHFTDVLTRCRTLLWNGPLGAFEVPPFGEGT
                     FALAKEVARLTKARKLVSIAGGGDTVAALNEAGVADQLTYVSTAGGAFLEWLEGRELP
                     GVAALARE"
     gene            5689..6375
                     /gene="gph"
                     /locus_tag="VF226_01725"
     CDS             5689..6375
                     /gene="gph"
                     /locus_tag="VF226_01725"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEX7072733.1"
                     /translation="MSERWPEAVVFDLDGTLIDSVGDITDALNGALLKTGLEPFSEAE
                     VRLMVGGGARVLVERVLNARNLSESVALAQQLYAAFMEIYQSGSVARTSVFDNARELL
                     EALRRQGIKCAICTNKPAHITNDVLAKLGMRDSFEVVVGGTEALPKKPHPAMLLAALA
                     ALGVDRSRAVMVGDSMADVGAARAADVPVIVVSHGYSKTPADELGADLVINSLSELSD
                     ALCKLIRRGS"
     gene            6520..7350
                     /gene="soxA"
                     /locus_tag="VF226_01730"
     CDS             6520..7350
                     /gene="soxA"
                     /locus_tag="VF226_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX7072734.1"
                     /translation="MRKSYTRLIAALGIAAFFAGTAPVNAQDEATEKGLEQYRKMLKE
                     DPWSNPGNLDVDRGETLWKTKAGPKNASLEQCDLGKGPGVVNGAFAELPRYFADADRV
                     MDVEARIVWCIDKLQGIPPKESVKPFPGGGRPTTDIGAIATYIASKSAGMKFAPHVEH
                     PKEKEMRAIGEEIFYRRQGPMDFSCATCHNDQGKRIRLQPLPYLANREEAKKVIGEWP
                     AYRVSSAHVMTMQHRIYDCFWQMRLPRIDYGSDVTVALTMYLADMARGGEISAPGLKR
                     "
     gene            7368..7988
                     /gene="soxX"
                     /locus_tag="VF226_01735"
     CDS             7368..7988
                     /gene="soxX"
                     /locus_tag="VF226_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX7072735.1"
                     /translation="MRKGLVALIVVGLTAGAAAYAEDKPAVDAIRVEQAIKESWKSAP
                     PEWQERLTQDQTMKECTESRNQPSNAVAEAIMKRERGKIVYPADGKFLGDWKKGEKLA
                     QSGYGLRFTDYPPRRENGGNCYACHQLTKSEVSYGTLGPSLLGYGKDRDYSEAEAKIV
                     YERIYNPQAHVACANMPRFGANKVLTIEQIKDIVALLMDPESPVNK"
BASE COUNT         1495 a         2455 c         2691 g         1400 t
ORIGIN      
        1 cacctggttc gagatcgccg atgagctgcg tcgctacgaa acgctgggcg gaaagggcgt
       61 tgcacccctg cgcgtcgggc cgcggccggc tcctcctgca gcgagcccgg ggggcgaggc
      121 gtgaaggcgc ctggaccgaa attccacatc gcggcgagca tttcctcgcg agggatgccg
      181 caagcggcta gaagatcgtc atgacgacca tcgattcacc gacacccgat cccgccgtta
      241 gcgtcggctc cgtgacgttc gcgaacgacc ggcaaatcgc gctctttgcc ggtccatgcc
      301 agatggaaag ccgcgcccat gcgctcgaaa tggcatcggc gctgaaggaa atttgcgcgc
      361 ggctgaacat cggcctcgtt tacaagacgt ccttcgacaa ggcgaaccgg acgagtcttt
      421 cgggaaagcg gggcatgggc ctcgaagcgg cactgcctgt ctttgccgag atccgtgaaa
      481 cattgggcct gccctgtgtc accgacgtgc acgaagcggc ccagtgcggc ccggtggccg
      541 aggtggtcga cgtgctccag atccccgcat tcctctgccg ccagacggat ctgctggtcg
      601 ccgcggcgaa aacaggtcgg gtggtgaagg tgaaaaaggg ccagtttctc gccccctggg
      661 acatgaagaa tgtcgtcgcc aagattacgg gcagcggcaa cacccgggtg ctcgtcacgg
      721 agcgcggggt cagcttcggc tacaatacgc tcgtggtgga catgcgcgcg ctgccgatca
      781 tggccgagac gggcgcgccg gtgatcttcg atgccacgca ttcggtgcag cagcctggcg
      841 ggcaggggac ggcgtccggc ggcgatcgcc ggttcgtgcc ggtgctggca cgggcggccg
      901 tggccgtggg cgtggcgggc ctgttcatcg aaacacatca ggatcctgac agagcgccat
      961 cggacggtcc taacatggtc ccgctgaacc ggctcgaatc cttgctggcc gagctgatcg
     1021 agctggatca cgtggtgaaa aagcaactcg catcggcccg ggatggggcg gcctgacaat
     1081 tcgattgcaa ttgaagctat gcttttgagt tcctgatgag gctgggcaac taagattacg
     1141 cctccgtcac gtattttgat ttaaactgat tgtccggcca cctgacggcg gctgatggga
     1201 tggacactat gaagcgaatc atctgcatcg gccattctgc gctcgattac atttatcgcg
     1261 tcgaagcctt cccgcggggg ccgtcgaaga tccgcgcgct ggagcatttc gagagcggcg
     1321 gcggcatggc ggccaatgcc gccgctacca ttgcgcggct gggcggcaag gtcgagctgt
     1381 ggagccgcgt cggcgacgac gaggcggcag ccaagatccg cgcggggctc aagagcttcg
     1441 gggtggacgt ccgctccgtc cagtccttcg aggaagggcg cacctcgaca tcggccatca
     1501 tcgtcgatga tgccggcgag cggctcatcg tcggggcgcg cgacatcaac atgccgtctg
     1561 ggacgagctg gctgccgctg gagcggatca gcggggcggc tgcggtgctg ggcgacgtgc
     1621 gctggctcga ggggctgcgc gttgtcttcg agcgggcacg aaaggaaggc attccgacgg
     1681 ttctcgatgc cgatctcggg gcacgggaag ctctcgccga aatcctggct ctcaccgact
     1741 acgccatctt ctccgagccc gcgctcaagg atttcgcggc tttgccgagc acggaggcca
     1801 tgctccagcg cgtgcgcgag ctcgggcccc ggcacgcggg cgtgacgctg ggtgcgcgtg
     1861 gctatcgctg ggtcgatgct tcaggcggcg gcgagcagcc cggcttcccc gtcgatgtgg
     1921 tcgacacgac gggtgctggc gacgcctttc acggggcatt caccctggcc cttgtcgaag
     1981 ggaagcccga agccgaatgc gcccgcttcg cctgtgccgt cgccgccatg aaatgcacgc
     2041 ggatcggctc ccgggccggc ctgccgacgc gggcgcaggt cgagggcttc cttgccgcgc
     2101 gaagctgatc caccgcccgt cctccagcac ccgccagcaa cagtcatacc ggggcttgtc
     2161 accagtatcc acctccccgc gagctccggc gtgtgttgag ggctggatcc cggcaacaag
     2221 tgccgttggt gggggatgct tggatttgcc ggtgtccaac ggaccgcgag ttcggcgttt
     2281 gttgaagggg ggatcccggc aacaagtgcc gggatgactg ttggtcggga cacccacgtt
     2341 tcgtgcgacc gcccatcagg tagacgcagc cactatcccc cgttaagagg gtcgcgcgac
     2401 agcgccgggc cggcgccgcg ccaggaccga acgaggtgag gacttcgatg ccccttgagc
     2461 aacacagggc tgccggcagc gccgaggtga gccataagga tatggccaat gccatccgcg
     2521 cactcgccat ggacgccgtc gaaaaagcga aatcggggca tccaggaatg cccatgggca
     2581 tggcggatgt cgccaccgtg ctcttcacga agttcctgcg cttcgacgcc gccgatccca
     2641 agtggcccaa ccgggaccgg ttcgtgttgt ccgccggcca tggatcgatg ctgctctacg
     2701 cgctgctcta cctgaccggt tatcccggaa tggatatcga gcagatcaaa cagttccgcc
     2761 agctcgggtc gaagacggcg gggcatcccg aatacgggca cgcggcgggc atagagacga
     2821 ccaccgggcc gctcgggcag ggcatcgcta cggccgtcgg catggcgatt gccgagcgca
     2881 tctggaacgc gcggctcgga cctgacgtta tcgaccacca cacgtacgtg atctccggcg
     2941 atggctgcct catggagggc atcagccagg aggcgatcac gctcgcgggc cacctcgggc
     3001 taggcaggct gatcgtgctg ttcgacgaca atggcatttc gatcgacggg ccgacggcgc
     3061 tatccacgtc cgatgatcag atccgccggt tcgaagccgc cggctggaat accgcatcga
     3121 tcgatgggca cgacccggag gcgatcgctt cggccatctc ccgcgcccgc tccgatgaca
     3181 gcaagccctg gctgatcgcc tgcaagaccg tgatcggcta cggcgcgccg aagaagcaag
     3241 ggaccgctgc gacgcacggc tctcctctcg gtgccgacga gatcgcagcg gctcgcgagc
     3301 ggcttggctg gccgtaccct ccgtttgaga tacctgagcc gattctgaat gcctggcggt
     3361 ctgcgggcaa gcgcggtgcg gcggagcggg caaattggca ggaacgctgg gccaaggtcg
     3421 atgccaagct gcgccagagc ttcgagcatc ctgctgccgc tgccggtgag gagttgcgga
     3481 aagcgatcgg cgaagcaaag cgcaaggccg cggccgagac ggccaagaaa gcaacgcgtc
     3541 agtggtcgga gctcacactc gatcacctgg tgccggtgct gccggggctg atcggcggct
     3601 cggccgacct gaccggctcg aacaatacga agaccaagtc gcaggccgtc atcaggaagg
     3661 gcgatttcag cggcagctat attcattacg gcatccgcga gcacggcatg gcggccgcca
     3721 tgaatgggct ggccctttac ggcggattga tcccgtacgg cggcacgttc cttgtgtttt
     3781 ctgactattg ccggccggcc attcgccttt cggccctgat gcgccagcgg gtcgtctacg
     3841 tgatgaccca cgattccatc ggtcttggtg aggacgggcc gacgcaccag ccgatcgagc
     3901 atctggcaag cctgagggcg atgccaaacc tcaacgtgct gcgcccggcg gatggggtcg
     3961 agacggccga gtgctgggag atcgccgttg ggccgggcga cacgcccagc atccttgcgc
     4021 tgacgcgcca agccgtgccg aacctgcgca ccacgcacac cgacgagaac ctttgcgcca
     4081 aaggcgcgta tgtcttgcgc gagcctgcgg gcacgcgcga cgtaacgcta ctggcgacgg
     4141 gctcggaggt cgggctcgcg gtcgaggcgg ccgaagctct ggcgaaagag ggagtcgcgg
     4201 cagcagtagt atcgatgccc tgcttcgagc tgttccggaa acagcccgag gattatcggc
     4261 gggccgtgct gggggaagct ccgcgcatcg gcatcgaggc cgccgttgcc cagggctggt
     4321 gcgaatggct cgacagaggc gacgtattca ttggcctcag cgacttcggc gcatccgcac
     4381 cggccgcagc cctgttccag cacttcggcc tgacgcccgg gcgcgtcgtt gaagcggcaa
     4441 aggccctgat cgcaaaacgc gggtgagcca gcggcacgag acgggagaac gccatggacc
     4501 tcgaaaagct caagacgacc gacggaatcg atgttcatgg aaagcgcgtt ctggtgcgcg
     4561 ctgatctcaa cgttcccgtg catggcggcg aggtgacgga cgtcacccgg ctggagcggc
     4621 tcgttcccgg actgctcgat ctggcaaatc gcggcgcgcg cgttgtggtc atctcgcact
     4681 tcggccgccc gaaaggcagt ccatcgcccg aattttcgct gaagcccgtt gccgcgaagc
     4741 ttggcgagct gctcggcaag ccggtcgcat tcgtcgagga ttgcgtcggg ccgaaggccc
     4801 aggcagcggt caacgcgctg gcgccggggc agttcgcagt tctcgaaaac ctgcgctttc
     4861 acaagggcga ggaaaagaac gatcccgaat tcgccaagga gctcgcgtcg ctcggcgaca
     4921 tctacgttga tgatgcgttc tcgagcgctc accgcgctca cgcctcgaca gagggcataa
     4981 cgcatcacct gccctcgtac gccggcccgc tgatgatggc ggagatcgag gcattgcgct
     5041 ccgcgctcga ccatcccgat ccccccaccg cggcaattgt cggcggtgcg aaggtctcga
     5101 ccaagattcc cgtcctgatg cacctgatag aaaaggtcga caagctcatc atcgccggcg
     5161 gcatggcgaa cacgttctat ctggcccagg gcgtcgacgt cggccgctcg ttggccgagc
     5221 ctgatttccg cgatacggcg ctcgatgtca tgaaggccgc caaggccagg agctgcgagg
     5281 tgatcctgcc gccggacgca gtcgtcgcgg ccgaattgaa ggagggcgtg ccgacccagg
     5341 tcgtctcgat cctcgacgtg ccctccgatg ccatgatcct cgacgtcggg ccgaagtcgg
     5401 tggagcactt caccgacgtg ctcacacgct gccgcacctt gctttggaat ggcccgctcg
     5461 gcgctttcga agtgccgcct tttggcgagg ggacgttcgc gctggccaag gaagtggcgc
     5521 gtttgacgaa ggcgcgcaag ctcgtttcca tcgcgggcgg gggagatacg gtagctgcgc
     5581 tgaacgaggc gggcgtcgcc gatcagttga cctacgtttc caccgcgggc ggcgcgttcc
     5641 tggaatggct cgaagggcgc gagttgcccg gcgtggcggc gctggcccgt gagtgagcgt
     5701 tggcccgaag ccgtcgtatt cgacctcgac ggtacactga tcgacagcgt cggcgatatc
     5761 acggatgcgc tgaatggggc gcttctgaaa acggggctgg agccgttctc cgaggcggag
     5821 gtgcgcctca tggtcggcgg aggcgcgcgc gttctggtcg aacgtgtcct gaacgcacgc
     5881 aacctgtccg aaagcgtggc gctggctcag cagctctacg cggccttcat ggagatctac
     5941 cagagcggtt ccgtcgctcg caccagcgta ttcgacaacg cgcgcgagct gcttgaagcg
     6001 ttgcgccgac agggcatcaa gtgcgcgatc tgtacgaaca agcctgcgca catcacgaac
     6061 gacgttctcg cgaaactcgg tatgcgggac agtttcgagg tcgttgtcgg cggcaccgag
     6121 gcgctgccca agaagccgca tccggccatg ctgctcgcgg cacttgccgc gctcggcgtc
     6181 gaccgcagcc gcgccgtcat ggtcggggac agcatggccg acgtcggagc ggcacgcgcc
     6241 gcggatgtac cagttatcgt cgtcagtcac ggctacagca aaacgcccgc tgacgagctc
     6301 ggtgcggatc tcgtgatcaa ttcgttgtcc gagctttccg atgcgctttg caaattgatc
     6361 cgccggggaa gctgattttc gctttttcgg ttcagctcgc ttaagcggcg cttgctcaag
     6421 gccgttatcg cacatattct gaaaacttga atgtcgagcc tgggcaggtg gaaatgccgg
     6481 cctttttggt gtcgctgagt ttcgagggag aggaaattcg tgcggaagag ctacacgcgg
     6541 ctgattgctg ctcttggaat tgcggcgttt ttcgcgggca ccgccccggt gaatgcacag
     6601 gacgaggcca ctgaaaaagg gctcgagcaa tacaggaaaa tgttgaagga ggacccctgg
     6661 tccaatccag gtaatctgga tgttgatcgc ggcgaaacgc tctggaagac aaaagccggc
     6721 ccaaaaaacg cttcgctcga gcaatgcgat ctcggaaaag gaccgggcgt ggtaaatggt
     6781 gcttttgccg agctgccgcg gtatttcgcc gatgccgatc gggtcatgga tgtggaggcg
     6841 cgtatcgtct ggtgcatcga taagcttcag ggcattccgc caaaggagtc cgtaaagcca
     6901 tttccggggg gagggcgtcc gactacggat atcggtgcaa tcgcaacgta tattgcgtcg
     6961 aaatcggctg gcatgaaatt cgctcctcat gtcgagcatc cgaaggaaaa ggagatgcgg
     7021 gcgatcggcg aggagatttt ctatcgccga caaggtccga tggatttttc atgcgctacc
     7081 tgccataacg atcagggcaa acgcattcgc ctgcagccgc tgccatatct cgccaatcgc
     7141 gaagaggcga aaaaggtcat cggcgaatgg ccggcttatc gcgtgtcctc ggcgcatgtc
     7201 atgacgatgc agcaccgaat ctacgactgc ttctggcaga tgcggctgcc gcgaatcgac
     7261 tatggatccg acgtgaccgt cgcgctcacg atgtacttgg ccgacatggc cagaggcggc
     7321 gagatatccg ctccgggtct caagcgctga acgagagggg gagtatcgtg agaaaaggtc
     7381 tcgtcgctct cattgtcgtc ggcctgacag ctggtgctgc agcctacgcc gaggacaagc
     7441 cggcggtcga cgccatacgg gtcgagcagg cgataaagga aagctggaag agcgcgccgc
     7501 ccgagtggca ggagcggctc acccaggacc agacgatgaa ggaatgcacc gagagccgta
     7561 atcagccttc caacgcagtg gcagaagcca tcatgaagcg cgagaggggg aagatcgtct
     7621 atcccgccga cggcaagttt ctcggcgatt ggaagaaggg cgagaagctg gcgcaatcgg
     7681 gctacggcct gcgctttacg gactacccgc cccgtcgcga gaacggcggc aactgctacg
     7741 cctgtcatca gctcacgaag agcgaggtga gctatggcac actgggcccg agcctgctcg
     7801 gctacggcaa ggaccgcgac tattccgaag cggaagcaaa gatcgtgtac gagcggatct
     7861 acaatccgca ggcgcacgtg gcatgcgcca acatgccgag gttcggggcg aacaaggtgc
     7921 tgaccatcga acagatcaag gacatcgtgg cgctgctgat ggatcccgag agccccgtca
     7981 acaagtagcg ggcgtcccgg gacgcggcga tcgcatgtcg cgggtggact tacggtcccc
     8041 c
//
LOCUS       DASVDU010000023        19609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1699535, whole genome shotgun sequence.
ACCESSION   DASVDU010000023 DASVDU010000000
VERSION     DASVDU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699535"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..81)
                     /locus_tag="VF226_01740"
     tRNA            complement(5..81)
                     /locus_tag="VF226_01740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Pro,seq:tgg)
     gene            226..1167
                     /locus_tag="VF226_01745"
     CDS             226..1167
                     /locus_tag="VF226_01745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7072736.1"
                     /translation="MSKIGEVRSTTRGHLLPVRPAALSLVTTSGFADYALLDSGRRRK
                     LERFGAITVDRPEPQAMWQPRLPSGKWSVAHAVFSGSEDEEKGRWRVDKPVPDSWPVR
                     VAGITMLCRLSAFRHLGLFPEQLPHWEWMLEGLQRVSGARPRVLNLFAYTGAASLIAA
                     KAGAEVVHVDASKKAIAWAKENQAASGLADAPIRWILDDARKFAAREVRRGRTYHVIL
                     VDPPKFGRGPGNEVWEIFEHLPDLMRDCAQLLDPAYARLCLTAYAIRASALAIDGLVR
                     DILADRGGPIDSGELAIVEQSGGRALGTSLFTRWSSR"
     gene            1164..1997
                     /locus_tag="VF226_01750"
     CDS             1164..1997
                     /locus_tag="VF226_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7072737.1"
                     /translation="MIRNRDRPASYRIVTSLQNPTVKAIRALEMRKVRRETGLFVAEG
                     ASVLMTAREHGWRPKTLLYQPSAAEGEVHRQLVEWASQTGAEILEVSEAVLGKVASKD
                     NPQSMLAVFPQAWHSPPDARKLAPDVVWIGLEEIRDPGNLGTIIRTADAVGAAGIMLI
                     GTCCDPYAREAVRASMGSIFAVPLVRINREEFLSWRKSWPGEVVGTHLAATEDFRAVP
                     YRGPTLLVMGSEGPGLSDALAAACDRLVKIPMAGKLDSLNLAVATALTLYQIRGPHLK
                     L"
     gene            2067..3005
                     /locus_tag="VF226_01755"
     CDS             2067..3005
                     /locus_tag="VF226_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7072738.1"
                     /translation="MRIEYHRTLIADRVRNDAFEVALARVIRKGETVVADIGAGTGLL
                     GLMASKLGAREVYLYEVAEVAAVAQEILKRNRARNCHLMPCHSTEMQDPPRADVVISE
                     TLGNYPFEEHIIETLNDALRRHLKRGGIIIPGRLTQFVAPVVTDRFHVELTAWERVGR
                     GIDFSPAQLMSLNNIYVRTLAPADLLDQGRSRKKWDEIDFTVQNRTTRKGEARWTPET
                     GTTIYGFATWWVAELVAGVTLSTAPDAPRTHWEQLYFPVLRPIEVRSGETVAISLRSR
                     SSMQAGTDVAWTVTRQDRAGKVVDRQAMDLAKGYLP"
     gene            complement(2993..3673)
                     /gene="rpe"
                     /locus_tag="VF226_01760"
     CDS             complement(2993..3673)
                     /gene="rpe"
                     /locus_tag="VF226_01760"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7072739.1"
                     /translation="MQDILIAPSILSADFARLGEEVRAIDSAGGDWVHIDVMDGHFVP
                     NITFGPKLVEAIRSHSQKIFDVHLMIAPCDPYIEPFAKAGADIITVHAEAGPHLDRSL
                     QLIKSIGKKAGVSLTPSTHESAIAHVLDKVDLVLVMTVNPGFGGQAFIPAMVEKVRNI
                     RQMIGERPIDLEVDGGINAETARDVATAGANVLVAGSAVFAGGRYAENIAAIRAAARA
                     ASGAGQGR"
     gene            complement(3957..4862)
                     /gene="rimK"
                     /locus_tag="VF226_01765"
     CDS             complement(3957..4862)
                     /gene="rimK"
                     /locus_tag="VF226_01765"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HEX7072740.1"
                     /translation="MRFVMLARNPRLYSHQRIVSAAQERGHVIDVINTLHVHMNITSS
                     RPTLRYGGHTLPLYDAVIPRIGASVTHYGLAVLRQFEMQGVYPLNESVAIGRARDKLR
                     ALQLLAREGIGLPVTAFAHGPRKAEDVIKEVGGAPVIIKLLEGTQGIGVILAETDASA
                     QSIIEAFSAANIDIMVQEYIREAQGSDIRAFVVGGAVVAAMQRTGRNGDFRSNLHRGG
                     NAVAVELSEEEQTTAVRAAEVMGLNVCGVDMLRSDRGPVVMEVNSSPGLKGIEQATGI
                     DVASLIIRFLEQSARPNQTKTRGNG"
     gene            4974..5471
                     /locus_tag="VF226_01770"
     CDS             4974..5471
                     /locus_tag="VF226_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7072741.1"
                     /translation="MAGREHRYRVSVRWTGNQGQGTTDYRSYSRSHEIASPANPDKAV
                     IAGSSDPAFRGDPARWNPEELYVAALSACHKLWYLHLCAVAGVVVESYVDEAEGIMVE
                     GEDGSGRFASVTLRPHITVTRSADIEKAQALHHEAHAKCFIANSVNTRVSIEPVISAS
                     KSPAG"
     gene            complement(5443..5658)
                     /locus_tag="VF226_01775"
     CDS             complement(5443..5658)
                     /locus_tag="VF226_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072742.1"
                     /translation="MPSRPEPSASETAASTRHDTYTRSVFKRVVLGVVLLFVMVSGWA
                     WLMNAGIEAEAETADDVAISQPATWTR"
     gene            complement(5868..7301)
                     /locus_tag="VF226_01780"
     CDS             complement(5868..7301)
                     /locus_tag="VF226_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072743.1"
                     /translation="MVTPIREFRLAYLPLLMIYFAYGALGITAVADSFWVKQSLTMTP
                     EDLASLGVWLTLPWTVKMVFGELVDAVPLLGSQRRAYVFLGASLVATGLVLLAGAAGG
                     WITFARPDTIYVLASLLTVTGVVLQDVVADAMSTEVVARNNSDGTPRDKAAVDRDLGM
                     VQVLGRLALSLGIFSVAWLSGYLAQVLSYQTVFLIGLAVPLISVTGAILVRLETAAPR
                     PVDWRLLAGGLAFGIVVTLLGVAGVPFSQEIIFIISMTVIILMLRRVTASVDDETRLR
                     IAYAAVVIFAFRAVPGVGAGYQWFSIDRLGFDEAFFGVLQQIGAGIGLVSAWLLSDTI
                     TRQPVARVMLWLTILGAVLAIPNLLLVYEVHHWTERVLGIGARQIAILDTAVQSPLIN
                     ISMIPMLTLIAIYAPAGHRATWFALMASLMNMALVAGQLLTKYLNMIFVVDRGDYVSL
                     PALTWASIILGLVIPLFAILRYGRWIR"
     gene            complement(7456..9009)
                     /locus_tag="VF226_01785"
     CDS             complement(7456..9009)
                     /locus_tag="VF226_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7072744.1"
                     /translation="MTLFSALLRSAARNGADRAAVEDVERNPLTYRRLVQGSMVLGRK
                     LAAITRRGENVGVMLPNVTALPLTLFALNAFGRVVAMLNFTAGVRNLKSAVKAAAVRT
                     VITSRKFIETAKLEQAIEALGNHETASGRQLEIIYLEDIRAHLTTADKAKGALQALAA
                     GAVHRWHALSPEQPAVILFTSGTEGEPKGVALSNRNLVANTQQIFAFGEKMLRTSDIF
                     FNPLPNFHAFGLTGGTLLPLFNGMKTVLYPSPLHYRQVARLIGETQATILVATDTFLQ
                     GYARAARPEDLASIRYAVAGAERVKPVTRGMWSRDGAVLLEGYGVTECSPVVALNLPD
                     TNRSGTVGRIIPGIEYRLAEVPGLKEGGRLSVKGPNVMLGYMLPSDPGVIVPPPGGWH
                     DTGDIVTIDKEGFVTIRGRAKRFAKVGGEMVSLAAVESIVCSLWPDGNHVVLSIPDPH
                     KGEQLVLVTDKPDADRDALIRYMQDEQISPLWLPKAILVIPAIPVLSSGKVDLMGATE
                     LVRQQLPLL"
     gene            complement(9086..10594)
                     /locus_tag="VF226_01790"
     CDS             complement(9086..10594)
                     /locus_tag="VF226_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7072745.1"
                     /translation="MRESGKDELELLTAAEMARADELTIRGGTPGLTLMENAGRAVAD
                     EIEWIAEPGARVAVLCGPGNNGGDGFVAARMLRERGYSVSVALLGAREALKGDAAQAA
                     EAWNGPIWPLSTAFAEDATVAVDALFGAGLSRPLEGIAAEVASSLDKHRVVAVDVPSG
                     VDGSTGEVRGVAVKAERTVTFFRRKPGHLLLPGRLHCGVTRVADIGIKADVLQQIGIS
                     TFANAPGIWLSLFPWPRLDGHKYARGHAVVVSGPAWRTGAARLAARGALRIGAGLVTL
                     ASDEDALAVNAAQVTAIMVRQANDAQDLAELLTDHRFNAVLIGPGAGVDDETRSKVLA
                     ILASGASVVLDADALTVFAEEREELFTAISAQRRPVVLTPHDGEFARVFPELTELPKL
                     DRVRLAARLSGAIVILKGADTVVAAPDGRAVINENAPPTLATAGTGDVLAGFVTGLLA
                     QHMPGFEAAAAAVWLHGAAAARFGPGLIAEDVPEMLPRILAELANGADAFVP"
     gene            complement(10606..10986)
                     /locus_tag="VF226_01795"
     CDS             complement(10606..10986)
                     /locus_tag="VF226_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7072746.1"
                     /translation="MRVTGFDHLVLTVASIERTADFYERVLGMTREVFGPERRTALRF
                     GPHKINLHERGKEFEPKAEAPTPGSADLCFLVEGFEDIADHLAAQGVPILAGPVERTG
                     ARRRIVSYYIRDPDLNLIELSHYP"
     gene            11301..11639
                     /locus_tag="VF226_01800"
     CDS             11301..11639
                     /locus_tag="VF226_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7072747.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEIGLQGITVLEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVLADELLDKAVEAIQKAAKTGRIGDGKIFISSIEEAIRI
                     RTGETGADAI"
     gene            11747..13156
                     /gene="glnA"
                     /locus_tag="VF226_01805"
     CDS             11747..13156
                     /gene="glnA"
                     /locus_tag="VF226_01805"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX7072748.1"
                     /translation="MANPADVLKLIKEKDVKFVDLRFTDTKGKMQHVTAEASCIDEGV
                     FADGYAFDGSSIAGWKGIEASDMLLSPDPATAHLDPFFAQTTLAIFCDVIEPSTGELY
                     ERDPRGIAKKAEAYLKQTGIGDTVYFGPEAEFFIFDDVRFTTDPFNTGFKLDSTELPA
                     NTGTAYEMGNLGHRPRTKGGYFPVPPIDSCQDIRSEMLSVMAEMGVAVEKHHHEVAAA
                     QHELGIKFAPMVKIADHMQIYKYVAHNVAQAYGKTATFMPKPVFGDNGSGMHCHQSIW
                     KDGQPTFAGNKYSDLSDTCLYYIGGILKHAKAINAFTNPTTNSYKRLVPGYEAPVLLA
                     YSARNRSASCRIPHVSSPKAKRVEVRFPDPAANPYLGFAAMLMAGIDGILNKIHPGEP
                     MDKNLYDLPPVELARIPTVASSLREAIQSLDEDRAFLKMGGVFSDDMIDAYIELKMAE
                     VLRFDTTPHPIEFDMYYSV"
     gene            13283..13708
                     /locus_tag="VF226_01810"
     CDS             13283..13708
                     /locus_tag="VF226_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7072749.1"
                     /translation="MDADQIIALLGLEPHPEGGYFRETFRDPRTIEGRACSTAIYFLL
                     KQGQTSHWHRVDASEVWHWYAGAPLELGIAPAGGPETIFRLGPALIEGERPQAVVPAD
                     HWQRAKSLGAWTLVGCTVAPGFTFSGFELAPPGFDPTAE"
     gene            complement(13830..14087)
                     /locus_tag="VF226_01815"
     CDS             complement(13830..14087)
                     /locus_tag="VF226_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072750.1"
                     /translation="MRELRSRIESVLQDYREEAANEERFVSEILTVLAMGSVARARMM
                     AEMAQVVARHTAGIRSATEDREFRKGIEDLAQFDYESTYKQ"
     gene            complement(14285..14935)
                     /locus_tag="VF226_01820"
     CDS             complement(14285..14935)
                     /locus_tag="VF226_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072751.1"
                     /translation="MLLRNHFALLLSLPALLLMSAAPTEAGKYDFRVTGVRRSDVLNI
                     RERVDDQATISQKKILGQIPANSSGVLGSGASIMVGSTRWYEIHFKGIRGWVNGRFLK
                     PTSSRLDDEFQSNLFCGGTEPFWALRIEGDTAELRRPEGPPVRYKVAVREPFQGRDDA
                     IAMRFVSDSGTDISALIQNKEWCNDGMSDLEYAFEAHIVGLSDANTPLQGCCSLLR"
     gene            complement(15427..16230)
                     /locus_tag="VF226_01825"
     CDS             complement(15427..16230)
                     /locus_tag="VF226_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072752.1"
                     /translation="MLRVGGLVALCLMLLALPAQAQRKADLVLLGEKSVGFLADRDTI
                     EVGKSEDWFRDRAFRTLHFAAERNDLHLMAVRVIYMNGYAEEIKVDRLIRAGGQLPID
                     LRGERSYLRRIEMVYRSRPSFRGQAVIKVYGEPAPRPLRPVRFQPDKDWLLLGEQKVG
                     FKTDHDVINVKQSENWFRNRAFRMLHFVVEDNELHLNSLKIVYFNGYTETIKIDRNIR
                     PGGHLAVDLRGERSYLRRIEMVYRSRPSFRGQAEVKVYGELAKRGRGRS"
     gene            complement(16455..16685)
                     /locus_tag="VF226_01830"
     CDS             complement(16455..16685)
                     /locus_tag="VF226_01830"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX7072753.1"
                     /translation="MAASYRAFLDMPGRRSLLGRLQTTVFSCWQGYVRWRDRRAALHH
                     LAELDDHMLKDIGISRSEIEWALYHGRSLKRR"
     gene            16846..17571
                     /locus_tag="VF226_01835"
     CDS             16846..17571
                     /locus_tag="VF226_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEX7072754.1"
                     /translation="MRRADRLFEIIQLMRRKAPVRARDLSEALEVSERTIYRDIQDLI
                     ASGVPIEGEAGIGYVLKAGFDLPPLMFKEQEIEALVLGARIVESWADAELAEAASNAI
                     AKIEAVIPDRLRDFMANTALLAPAHSYMEPITFDLAELRRALRSQLKIHFRYRDFMAR
                     ETTRTVRPLSLAYFGPVWLLAAWCELRDDFRTFRLDRIDQLQILNERFRIEQGKSLHD
                     FLKRDQAWNRRSETVTSMETGND"
     gene            17612..18169
                     /locus_tag="VF226_01840"
     CDS             17612..18169
                     /locus_tag="VF226_01840"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7072755.1"
                     /translation="MKAPKKIDFPPWNVRRFLEPGPVVLVSSAYRDQRTIMTMGWHMI
                     MEMQPALVGCYIWDQNFSQKLVRGSKECVINLPTSDLLETVIKIGNCHGNEVDKFNEF
                     GLTAAEAETVGAPLIAECYANFECKLVDSSLIRKYNLFVFEVTKAHVARSPRYPETVH
                     YRGEGVFMISGENRSYRRLFKPEML"
     gene            complement(18170..>19609)
                     /locus_tag="VF226_01845"
     CDS             complement(18170..>19609)
                     /locus_tag="VF226_01845"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEX7072756.1"
                     /translation="TPEAAVDRLEELYDRASTALVRALDRYLETREPPTEKERAAFRY
                     PLLRVIYYGMDPAPRTRRAYAKFQRPGVYETTITHPRHFRNYLLEQLRLLVGEYGAEI
                     EVSVSTQEIPFPYTMERVEEIGRKGITASELARHFPTPLLSLVGDEVADGEWQAPEGE
                     PRPLSLFDAVRVDYSLRRIVHYTGSDWRNVQHWILLTNYHRYVDQFVRWGLARLAEAN
                     TPYERLVLPGGAEIRRGDSPDDADGVIAASPWHRFQMPAYHLTAPSGEGVTLVNIGVG
                     PSNAKNITDHLAVLRPNCWLMVGHCGGLRQSQQIGDYVLAHAYLRQDQILDALLPTDI
                     PIPALAEVQVALQDAAAIVTGEKGDALKGRLRTGTVVTDVDRNWELRYSHERRRINLS
                     RAIAVDMESGTIAANGYRLRVPYGTLLCVSDKPLHGEIKLPGAANAFYEAAVAQHLEI
                     GLKALDLLRERRETLHSRKLRSFDEPPFR"
BASE COUNT         3746 a         6275 c         6133 g         3455 t
ORIGIN      
        1 tgattggtcg gggcggaggg attcgaaccc ccgaccccct gctcccaaag caggtgcgct
       61 accagactgc gctacgcccc gacatgccaa aaggggtttg atttctgggg cgtcccgagg
      121 ctgaggtcaa gccccaaggc gatccgtctg cacacgcaaa gcattgacat cccgtggcgc
      181 ttccggaatg acgtcgggcg acttggacgg ccggcggcgc gggtcatgag caaaattggg
      241 gaagtcagaa gcacgacgcg cggccatctg ctgcctgtgc gaccggcggc gttgtcgctc
      301 gtcacgacaa gcggatttgc tgactacgcc ctgctcgatt ctggccggag acgcaagctc
      361 gagcggttcg gcgccatcac cgtggatcgg ccggagccgc aggcgatgtg gcagccgcga
      421 ttgccctcgg gaaagtggtc ggtggcacat gccgtgttct ccggcagtga ggacgaggag
      481 aaaggccgct ggcgcgtcga taagccggtg cctgattcct ggccggtcag ggtcgccgga
      541 atcacgatgt tgtgccggct cagcgcattc cggcatctgg ggctctttcc ggagcagcta
      601 ccgcattggg aatggatgct cgaaggcctg caacgggtct ccggagcgcg cccgcgcgtg
      661 cttaaccttt tcgcctacac cggggccgcc tcgctgatcg ccgcaaaggc cggcgcggag
      721 gtcgtccacg tcgatgcctc gaagaaggcg atcgcctggg cgaaggagaa tcaagccgcc
      781 tccggcctcg ctgatgcacc gatccgttgg attctggacg atgcccgaaa gttcgccgcg
      841 cgggaggtgc gccgcggccg gacgtatcac gtgatcctcg tcgacccgcc gaaattcggg
      901 cgcggacccg gcaacgaggt ctgggagatc ttcgagcatt tgccggacct catgcgtgac
      961 tgcgcgcagc tgctcgatcc ggcatacgcc cgcctatgcc tcacggcgta cgccatccgc
     1021 gcctcggcgc tcgccattga cgggctcgtg cgcgacattc ttgccgatcg cggtggaccg
     1081 atcgacagcg gcgagctggc catcgtcgag cagtcgggcg ggcgcgcgct cggcacgtct
     1141 ctcttcacac gctggtcctc acgatgatcc gcaatcgcga ccggcctgcc tcctaccgca
     1201 tcgtcacgag cctgcagaac ccgaccgtca aggccatccg cgctctcgag atgcggaaag
     1261 tgcgccgtga gaccgggctg ttcgtcgcgg aaggcgcatc ggtgctgatg acggcgcgcg
     1321 agcacggatg gcggccgaaa acgctgcttt atcaaccaag tgccgccgaa ggcgaggtcc
     1381 accgccagct tgtcgagtgg gcgagccaga caggcgccga gatcctggag gtcagcgagg
     1441 cggtgctcgg caaggtggct tcgaaggaca acccgcagag catgctcgcg gtcttcccgc
     1501 aggcctggca ctcaccgccg gatgcgcgca agctggcgcc ggatgtggtt tggatcgggc
     1561 tcgaggaaat ccgcgacccg ggcaatctcg gcaccatcat ccggaccgcc gacgcggttg
     1621 gtgcggccgg gatcatgctg atcggcacct gctgtgaccc ctacgcgcgc gaagcggtgc
     1681 gggcgagcat gggctcgatt ttcgccgttc cgctcgtgcg gataaaccgc gaggagttct
     1741 taagctggcg gaagtcgtgg cccggtgaag tcgtgggcac ccatctggct gcgacggagg
     1801 attttcgtgc cgttccctat cgcggtccga ccctgctggt gatgggcagc gagggccccg
     1861 gattgtcaga tgcgctcgcc gccgcatgcg accggctggt caaaattccg atggccggca
     1921 agctcgacag cctgaacctt gcggtggcga ctgccctgac gctttatcaa atccgcggcc
     1981 cacatctgaa actttgataa ttcaggcaac catcccctac ccgcaattcg ctattcgcta
     2041 ttcgcaactc accattcgct cccctcatgc gcatcgaata ccaccgcacg ctcattgcag
     2101 accgcgtccg caacgacgcg ttcgaagtgg ccttggcacg ggtgatccgg aagggcgaaa
     2161 ccgtcgtggc cgacatcggc gccggcaccg ggctgctcgg cctcatggcg tcgaagctcg
     2221 gggcgcgtga ggtctacctc tatgaagtcg ccgaggtcgc ggcagttgcc caggagatcc
     2281 tgaagcggaa cagagcgcgc aactgccacc tgatgccgtg ccactcgact gagatgcagg
     2341 acccgccgcg agccgacgtg gtcatttcgg agacactggg gaactatccg tttgaagagc
     2401 acattatcga gacgctgaat gacgccttgc gccggcatct gaaacgcggc ggcatcatta
     2461 ttccaggccg tctcactcaa ttcgtcgctc cggtcgtgac ggaccgattt cacgtcgagc
     2521 tgaccgcctg ggagcgtgtc gggcgcggaa tcgacttctc acccgcgcaa ctcatgagcc
     2581 tcaacaatat ctacgtgcgg accctcgcac ccgcagacct gctggaccag ggccgctcga
     2641 ggaagaaatg ggacgagatc gacttcacgg ttcagaaccg aacgacgcgc aaaggcgagg
     2701 cacggtggac gccggaaacg ggcacgacga tctacggctt tgccacgtgg tgggtcgcgg
     2761 agctggtcgc cggcgtcacg ctctccaccg cgcccgacgc tccgcgcacg cattgggagc
     2821 aactctattt tcccgtcttg aggccgatcg aggtccgcag tggagagacc gtcgcgattt
     2881 cgctgcgctc gcgctcgtcc atgcaagccg gcacggatgt ggcatggacc gtcacgcggc
     2941 aagatcgtgc cggcaaggtc gtcgaccgtc aggcgatgga tctggcgaag ggctacctgc
     3001 cctgaccggc gccggaagcc gcgcgagccg ctgcacggat cgccgcaatg ttctcggcgt
     3061 aacgcccgcc tgcaaaaacg gcacttccag caaccagcac attggcgccg gccgttgcga
     3121 cgtcgcgcgc tgtctcggca ttgatgccgc cgtcgacctc cagatcgatc gggcgctccc
     3181 cgatcatctg acgaatgttt ctcaccttct cgaccatcgc cgggatgaag gcttggcctc
     3241 caaagcccgg attgaccgtc atgaccagca cgaggtcgac cttgtcgagc acgtgagcga
     3301 tggcgctctc gtgcgtcgag ggcgtcagcg aaacgcctgc cttcttgccg atgctcttga
     3361 tgagttgcag cgatcgatcg agatgcgggc ctgcctccgc atgcaccgtg atgatgtcgg
     3421 caccagcctt cgcaaaaggc tcgatgtaag gatcgcaggg ggcgatcatc aggtggacgt
     3481 cgaagatctt ctgcgaatgc gaccggatcg cttcgaccag cttgggaccg aacgtgatgt
     3541 tcggtacgaa atggccgtcc atcacgtcga tatgcaccca atcgccgcct gccgagtcga
     3601 tggcgcgcac ctcctcgccg agccgcgcga agtcggccga aaggatcgaa ggggcgatga
     3661 ggatgtcctg catgctggag tccatttact gtggcgcgct gtgccggata gcaccttcgg
     3721 agccggaggt ggcctgtcta gctgttacgc cactctccct ttccccggtg gggagagggt
     3781 cggggtgagg gggttcccac aactgctggc gtcgtacccc ctcacccggc ccttcgggcc
     3841 gacctctccc cacgggagag gtgaatggcg tcgtccatgg cgcttcgaca ggaccgaaac
     3901 gaccaaatcc gcccggcagc cgatagcatc ccgatgccgc ggtgggaagg cggtgatcag
     3961 ccgttccccc gcgtctttgt ctggttgggc cgggcgcttt gctcgagaaa tctgatgatc
     4021 aggcttgcga catcgatgcc ggtcgcctgc tctatcccct tcaaacccgg cgaggagttg
     4081 acctccatga ccaccggccc gcgatccgag cgcagcatgt ccacgccgca gacgttgagg
     4141 cccatgacct cagccgcccg cacagcggtc gtctgctcct cctcgctcag ctcgacggcg
     4201 acggcattgc cgccgcgatg caggttggag cggaagtcgc cgttgcgtcc ggtgcgctgc
     4261 atggctgcca cgacggcgcc gccgaccacg aaggcgcgaa tgtcggagcc ttgcgcctcc
     4321 cggatgtact cctgaaccat gatgtcgatg ttcgcggcgc tgaatgcctc gatgatggac
     4381 tgggcggacg cgtcggtctc ggcaaggatc acgccgatgc cttgcgtgcc ctccaagagc
     4441 ttgatgatga ccggggcgcc gccgacctcc ttgatgacgt cctcggcctt gcgcggcccg
     4501 tgcgcgaagg ccgtgacggg caggccgatg ccctcgcgcg ccaggagctg cagcgcccga
     4561 agcttgtcac gcgcgcggcc gatcgcgacg ctctcattga ggggataaac gccttgcatc
     4621 tcgaattggc gcaggaccgc cagcccgtag tgggtgacgg aagcgccaat acgagggatc
     4681 accgcgtcat agagcggcag cgtatgcccg ccgtaacgca gcgtcggccg gctcgacgtg
     4741 atgttcatat gcacatgcag ggtgttgatc acgtcgatca cgtgaccacg ctcctgcgcg
     4801 gcagagacga tgcgctggtg cgagtaaagt cttggatttc gcgccagcat cacgaaacgc
     4861 atgaacaagc tccacaaccg accgccgcac tcgaactgga ctgggcgcag tgataggcta
     4921 ccgcgtggtt cgggccaagc gcctcgaaag aggtccaatg ggagctaaag cgcatggctg
     4981 ggcgtgagca tcgttatcgg gtgagcgtcc gctggacggg caaccagggg caaggcacga
     5041 cagattatcg aagctattcc cgcagccatg agatcgctag cccggcaaac ccggacaagg
     5101 cggtcatcgc gggctcctcg gatccggcat tccgcggaga tcccgcccgc tggaatccag
     5161 aagagttata cgtggcagca ctctccgcct gtcacaagct ctggtatctg cacctgtgcg
     5221 ccgtggccgg cgttgtcgtc gaaagctacg tcgatgaggc cgaggggatc atggtcgagg
     5281 gcgaggatgg ctccggccgc ttcgcctccg taacgctgcg gcctcacatc accgttacgc
     5341 gcagcgcaga tatcgaaaag gcgcaagcct tgcatcacga ggctcacgcc aaatgcttca
     5401 tcgccaattc ggtcaacacg cgggtgagca tcgagcccgt gatcagcgcg tccaagtcgc
     5461 cggctggctg atggctacgt cgtctgccgt ttcggcctcg gcttcaatcc cggcgttcat
     5521 gagccaggcc caaccggaga ccataacgaa caacagcacc acgcccagca ccacccgctt
     5581 gaacacggag cgggtatagg tgtcgtgacg agtgctagca gcagtctctg atgcagatgg
     5641 ttcgggtcgt gacggcattg cttctccccc cgagcaagcg aacaggggtc gtggctgatg
     5701 actgtgacgc ggtcaagatg gtctgctcgg cgtcggcgtt ttcgttatgg cgccgtgatc
     5761 cgggcgacac gggctcgagc accattgtcg accctcacaa cgcgcgcccc tgccttcggg
     5821 atccggcccc tgcactgaaa tgtctcagaa agcactaacg agcaggctca gcggatccac
     5881 cggccgtagc ggagaatggc gaagagcggg atcacgagac caaggatgat gctggcccag
     5941 gtcagcgccg gcaaggagac ataatcaccg cggtccacaa cgaaaatcat gttgaggtac
     6001 ttcgtcagga gctggccggc gacgagggcc atgttcatca gcgaggccat cagggcaaac
     6061 caggtagcgc gatggccggc aggcgcgtaa atcgcgatca gtgtcagcat ggggatcatg
     6121 ctgatgttga tcagcggcga ctgcaccgcg gtgtccagga tagcgatctg gcgagcgccg
     6181 atgccgagca cccgctcggt ccagtggtgg acctcgtaga ccagcagaag attggggatc
     6241 gcgagcacgg caccgaggat ggtgagccac agcatcacgc gggccaccgg ctgacgcgta
     6301 atggtgtccg acaggagcca ggctgaaacc aagccgatgc cggcaccgat ctgttgcaga
     6361 acgccgaaga acgcctcgtc gaacccgagc cggtcgatcg agaaccactg atagccggcc
     6421 ccgacacccg gcacggcccg gaacgcgaat atgaccacgg ccgcatacgc gatcctgagc
     6481 cgcgtctcgt cgtcgacgct ggccgtgacg cggcgcagca tcaggatgat caccgtcatc
     6541 gaaatgatga agatgatttc ctgactgaag ggtacaccgg cgacaccgag gagtgtcacg
     6601 acgatgccga acgcgagccc gccggcgagc aggcgccagt ccacgggtcg gggcgctgcc
     6661 gtttcaaggc gcacgagaat ggcgccggtg accgagatca acgggacggc aagtccgatc
     6721 aggaacacgg tctgataaga aaggacttgg gcgaggtaac ccgaaagcca cgcaaccgag
     6781 aagatgccga gcgacaacgc cagtcgcccc agcacctgca ccattccgag atcgcgatcg
     6841 acggcagcct tgtcgcgcgg tgtaccgtcc gaattgtttc gggcaaccac ctcggtgctc
     6901 atggcatcgg cgaccacgtc ctgcaaaacg acgccggtca cggtgagcag cgaggccaga
     6961 acatagatcg tgtccggtcg cgcgaaagtg atccagcccc ccgccgctcc ggcgagcagc
     7021 acgagccctg ttgcgacgag gctggcccca aggaagacgt aagcgcggcg ctgggagccg
     7081 agcagcggca cggcatcgac gagctccccg aagaccattt tcaccgtcca cgggagtgtc
     7141 agccagacgc cgagcgaggc caggtcctcc ggcgtcatgg tgagcgattg cttcacccag
     7201 aagctgtcgg ccaccgccgt gatgccgagc gccccatagg cgaaatagat catcagcaat
     7261 ggcagataag cgaggcggaa ctcacggatg ggcgtaacca cggccgcctc gaggcgctgc
     7321 ctccaggtat ccgggcgctc ggccgccaga gcgtgtggtt cgagtgatgc tgtcacgatt
     7381 cgaagagtgg gagccgctgt tgcctgcgac aagacctgcg gcgaagccgc agtcggcagc
     7441 tcgttggagc gcggctcaca acagcggtag ctgctggcgc acgagctcgg tcgctcccat
     7501 caaatcgacc ttgccggagc tcagcaccgg aatggcagga atgacgagaa tggctttggg
     7561 cagccaaaga ggtgagatct gctcatcttg catgtaccgg atcagggcat cgcggtcggc
     7621 gtccggcttg tcggtcacca gcacgagctg ctcgcccttg tgcggatcgg gaatgctgag
     7681 gacgacgtgg ttgccgtccg gccagagact gcagacgatg gactcgacgg cggccagaga
     7741 caccatctcc ccgccgacct tggcaaaccg cttcgcgcgg ccccgaatgg tgacgaagcc
     7801 ttccttatcg atcgtgacga tgtctccggt gtcgtgccat ccgccgggcg gaggtacgat
     7861 cacccccggg tcactgggca gcatgtagcc cagcatgacg tttggcccct tgacggacag
     7921 cctgccaccc tccttcaggc ccggcacctc tgccaggcga tactcgattc ccgggatgat
     7981 cctgccgacc gtgccgctgc ggttggtgtc cggcaggttc aatgccacga cgggcgagca
     8041 ttcggtgacg ccatatccct cgagaagcac ggcgccgtcc ctcgaccaca tgcctcgggt
     8101 cacgggcttc acgcgctcgg cgccagccac ggcatagcga atgctggcca ggtcttccgg
     8161 ccgcgcggcg cgcgcatagc cctgcaggaa cgtgtcggtg gcgaccagaa tagtggcctg
     8221 cgtttcgccg atgagccgcg ccacctgccg gtaatgcaat ggactggggt agaggacggt
     8281 cttcatgccg ttgaacaggg ggagcagcgt gccccctgtg aggccgaagg catggaagtt
     8341 cggcaacggg ttgaagaaga tgtccgaggt gcggagcatc ttctccccga aagcgaagat
     8401 ctgctgcgtg ttggccacca ggttgcggtt cgacagagcc acgcccttcg gctcgccctc
     8461 ggtgcccgag gtgaacagaa tgaccgccgg ctgctcgggg ctgagcgcgt gccagcggtg
     8521 gaccgcgccc gcggcaagcg cctgcagggc ccccttcgcc ttgtccgccg ttgtcaggtg
     8581 tgcccgaata tcctcgagat agatgatctc gagctggcga cctgacgccg tctcatggtt
     8641 accgagtgcc tcgatcgcct gctcgagctt ggcggtctcg atgaactttc gagaggtgat
     8701 cacggttcga accgcggctg cctttactgc ggatttgagg ttccgcacgc ccgcagtgaa
     8761 attcagcatc gccacgacgc ggccgaaagc gttgagagcg aacagcgtca gcggcaaagc
     8821 ggtcacgttc ggcagcatga cgccgacgtt ttcaccgcgc cgcgtgatcg cagccagctt
     8881 cctgcccagc accatgctgc cctgcacgag ccggcgatag gtgagcggat tccgctccac
     8941 atcctcgacc gccgcgcgat cggccccatt ccgcgccgct gaccggagca gcgcggaaaa
     9001 cagtgtcatg cgagaggctt ccggatcgaa ctgcgtcata cacgaaatat ggcgtcaaag
     9061 gccggcatat gaaacacgaa tgccctcaag ggacaaaggc atcggccccg ttcgccagct
     9121 ccgccaggat gcggggcagc atctccggca catcctccgc gatcaggccg gggccgaacc
     9181 tggctgccgc agccccgtgc agccagacgg ccgcagcggc agcttcgaag cccggcatgt
     9241 gctgtgccag aagccccgtc acgaaaccgg ccagcacgtc gcccgtaccc gccgtggcga
     9301 gggtcggcgg cgcattctcg ttgatcacgg cgcgaccgtc gggcgccgcg accaccgtgt
     9361 ccgcgccttt gaggatcacg atggccccgc tgagacgcgc tgccagccgc acccggtcaa
     9421 gcttcgggag ctcggtcagc tccggaaaga cgcgggcgaa ctcgccgtca tggggcgtga
     9481 gcaccaccgg gcgccgctgg gcggaaatgg ccgtaaacag ctcctcgcgc tcctcggcaa
     9541 aaacagtgag cgcgtcggca tcgagcacta cgctggcgcc tgatgccaga atggccagca
     9601 ccttgctccg cgtttcatcg tccacgcccg cgccggggcc gataaggacc gcgttgaagc
     9661 gatggtcggt cagcagctcg gccaggtcct gcgcgtcatt tgcctgacgc accatgatcg
     9721 ccgtgacctg ggccgcgttg acggcaagcg catcctcgtc gctcgcgagc gtgacgagcc
     9781 cggcaccgat ccgtagcgcc ccgcgcgcgg ccaatcgcgc cgctcccgtc cgccaggccg
     9841 gacccgacac cacgaccgca tggcccctgg cgtacttgtg cccatcgagc cgcggccacg
     9901 ggaaaaggga tagccaaatt cctggtgcat tggcgaacgt cgagatgccg atctgctgca
     9961 atacatcggc cttgatgccg atgtcggcca cgcgggtcac tccgcaatgg agacggccgg
    10021 gcaggagcaa gtgcccgggc ttcctccgga agaaggtaac tgtgcgctcc gctttcacgg
    10081 ccacgcccct gacctcgccg gtgctgccgt caacgccgct cggaacgtcg accgcgacga
    10141 cccggtgctt gtcgagactg gatgccacct ctgcggcaat gccttcgagc ggccgggaga
    10201 gaccggcgcc gaacagggca tcgacggcga cggtcgcatc ctcggcgaat gccgtgctga
    10261 gcggccagat cggcccgttc caggcctcgg ccgcctgagc cgcatcgccc ttcagcgcct
    10321 cgcgcgcgcc gagcagcgcc acactgaccg aatagccgcg ttccctcagc atgcgggcgg
    10381 caacgaaacc gtcgccgcca ttgtttcccg gcccgcagag cacggcgacg cgtgcgcccg
    10441 gctccgcgat ccactcgatc tcatcagcca cagcacggcc ggcgttctcc atcagcgtca
    10501 gccccggcgt gccaccgcgg atggtgagct cgtcggcgcg cgccatttcc gccgcagtca
    10561 gcagctccag ctcatccttg ccgctctcgc gcatcgcctg ctccgctagg ggtagtggct
    10621 caactcaatc agattgaggt ctgggtcgcg gatgtaatag gaaacgatcc ggcggcgcgc
    10681 gccggtccgc tccaccggtc cagcgaggat gggcacccct tgcgctgcca agtggtccgc
    10741 aatgtcctca aaaccctcca caaggaaaca caggtccgcc gaccccggcg tcggagcctc
    10801 ggccttcggc tcgaactcct tccctcgctc gtggagattg atcttgtgcg gcccgaagcg
    10861 cagggcagtc cggcgttcag ggccaaacac ctcccgcgtc atgccgagga cgcgctcata
    10921 gaagtccgcc gtacgttcga tcgaggcgac cgtcaggaca aggtgatcga agcctgtgac
    10981 gcgcaagggc tctcctccgc aaattgggtt gatcgttctg agccacgagg cgggctcagc
    11041 ggccaccgag tcaagtccgg cgcccaaaat tgtggttgag caaaggattg caattcatcg
    11101 cggcccgaat tctcgctgca taaaaaaacg gcataaagac taaagatgcg gcaatgcaca
    11161 cgccaccgtc tctcgaccgg cgaaccagga cagggctaaa ccattgataa aacagctcag
    11221 atctcggcca gcgagcatct ggcacgcctc atgctagttc gaggccgagc caacggacag
    11281 cccgctgcgg gaggcacgga atgaagaaga tcgaggccat cattaaacct ttcaagctcg
    11341 acgaggtgaa ggaggccctt caggagatag ggttgcaagg catcactgtc ctcgaggcca
    11401 agggcttcgg ccgccagaaa ggtcataccg agctttaccg cggggccgag tacgtcgttg
    11461 acttcctgcc caaggtcaaa atcgaggtcg tcctggcgga cgagttgctc gacaaagccg
    11521 tcgaagccat tcagaaggcg gccaaaacgg gccgcatcgg cgacggcaag atcttcatct
    11581 cgtcgatcga ggaagcaatc cggatccgca ccggggaaac cggcgcagat gccatttgag
    11641 agcgaagtcg ccaatctgca gaggcagctt ttgcctcgct gcaggaagaa atcctgagca
    11701 cccaaaaccg agcccgaacc aagcctgcat agaggggagt tgttaaatgg caaatccagc
    11761 cgacgtcctt aagctcataa aagaaaagga cgtgaagttc gtcgacctcc ggtttaccga
    11821 taccaagggc aagatgcagc acgtcaccgc ggaggcgagc tgcatcgatg aaggcgtttt
    11881 cgcggacggc tatgccttcg acggctcgtc gatcgcgggc tggaaaggta tcgaggcctc
    11941 cgacatgctg ctgagccccg atccggcgac ggcgcatctc gacccgttct tcgcccagac
    12001 cacgcttgcc atcttctgcg acgtgatcga gcccagcacc ggtgagctct atgagcgtga
    12061 tccgcgcggg attgcgaaga aggcggaagc ctacctcaag cagaccggca tcggtgacac
    12121 cgtatacttc gggcccgagg cggagttctt cattttcgat gacgtccgtt tcacgaccga
    12181 tccgttcaac acgggcttca agctcgactc gacggagctg ccggcgaaca ccggcaccgc
    12241 ctacgagatg ggcaacctcg gccaccggcc gcgaacgaag ggaggctact tccctgttcc
    12301 gccgatcgac agctgccagg atatccgttc cgagatgctg tcggtgatgg ccgaaatggg
    12361 cgtggcagtg gagaagcacc accacgaggt tgctgccgcg cagcacgagc tcggcatcaa
    12421 gttcgcgccg atggtgaaga tcgcggacca catgcagatc tacaagtacg tggcccacaa
    12481 cgtcgcccag gcctacggca agacggcgac cttcatgccg aagccagtgt tcggcgacaa
    12541 cggctcagga atgcattgcc accagtcgat ctggaaggac ggccagccga cgtttgccgg
    12601 caacaagtac tcggaccttt cggacacctg cctctactac atcggcggta tcctgaagca
    12661 cgccaaggcg atcaatgcct tcacgaaccc gacgacgaat tcctacaagc gtctggttcc
    12721 gggctatgag gcgcccgtgc tgctggccta ctcggcccgc aaccgttcgg cctcctgtcg
    12781 catcccgcac gtgtcgagcc cgaaggcgaa gcgcgtagaa gtccgcttcc ccgacccggc
    12841 cgcgaatccg tacctcggct ttgcagccat gctgatggcc ggcatcgatg ggatcttgaa
    12901 caaaatccac cccggcgagc cgatggacaa gaacctctac gacctgccgc cggtcgagct
    12961 ggcccgcatt ccgacggttg ccagctcgct gcgggaggcg atccagagcc tcgacgagga
    13021 tcgcgccttc ctgaagatgg gcggcgtgtt ctcggacgat atgatcgacg cctacatcga
    13081 gctgaagatg gcggaggtcc tacgtttcga caccacacct cacccgatcg agttcgacat
    13141 gtactattcg gtgtagtctc ggtttctgca ccaacgcaaa atgccagggc ggctcgtcgg
    13201 gccgccctta atttttctcg agtgggctaa tgataggctt tggacctggt ccgatagcgg
    13261 agctttcgac gagaatagga tgatggatgc ggatcagatc attgcgcttc tggggctcga
    13321 gccgcatccg gagggcggat acttccgcga gacgttccgg gatccgcgga cgatcgaggg
    13381 gcgcgcctgc tcgacggcga tctatttcct gctgaagcag ggtcagacct cccattggca
    13441 tcgcgtggat gcgagcgaag tctggcattg gtacgctggt gcgccgctcg agctcggcat
    13501 cgctcccgcc ggcggccctg agacgatttt ccggcttggc ccggcgctta tcgagggcga
    13561 acgcccccag gccgttgtcc ccgccgatca ttggcaacgc gcgaaaagcc ttggggcgtg
    13621 gacgctcgtc ggatgcacgg tggcccctgg ctttacgttt tcaggatttg aactcgctcc
    13681 tccggggttt gatcccacgg cggagtgagc agcactcagc ctgttcgttg gcaagctgga
    13741 gcgggagagc ctcgcgacgc caatcaattc agcagcgcgc agcgatccaa ccaacccctt
    13801 cgatggagtt ggattccaat cgcgccgccc tactgcttgt aggtgctctc gtagtcgaac
    13861 tgcgcgaggt cttcgatgcc tttgcgaaac tcgcggtcct cggtcgccga gcggattccg
    13921 gccgtatgcc tggccaccac ctgcgccatc tcggccatca ttctcgcacg cgcgacgctc
    13981 cccatcgcca gtacggtcag aatttccgag acaaaccgct cctcgttggc ggcttcttcc
    14041 cggtaatcct gcagcaccga ttcaattcga ctgcgtagct cgcgcatgct cgaccccctc
    14101 agtgcccgag accagtctgt ttgccctctt cagtcccggc ttaggtcgat agttgcccga
    14161 ttcgtcgtga cttttccatg cattagctgt ttcgtctgag cctattcttc gaacaacggc
    14221 aattcgtctt acagaaacac agatttcatg aattatcgct ggagttttcc tcgactattg
    14281 caactcatct taggaggctg cagcagccct gaagcggcgt attggcgtcc gacaagccga
    14341 cgatgtgggc ttcgaacgca tactcgaggt ccgacatacc gtcgttgcac cactccttgt
    14401 tctggatcag ggcgctgatg tcggtccccg aatcgctgac gaaccgcatg gcgatggcgt
    14461 cgtcgcgacc ctggaaaggt tcgcggaccg caaccttata gcggaccggc ggtccttccg
    14521 gccggcggag ctctgccgtg tccccttcga tcctgagagc ccagaacggc tcggtgccgc
    14581 cgcagaagag gttggactgg aactcatcat ccaatctgga cgaagtcggc ttcagaaaac
    14641 gcccgttgac ccaaccgcgg atgcccttga agtggatttc gtaccagcgc gtcgagccga
    14701 ccatgatact ggctcccgac ccgagcacgc cgctgctgtt ggcggggatc tggccgagaa
    14761 tcttcttctg actgatcgta gcctgatcgt cgacccgctc acgaatgttc aggacgtcgg
    14821 accgcctaac ccccgtcacg cggaaatcat acttgccggc ctcggtaggc gcggctgaca
    14881 tcagcagcag tgccggaagg cttagcagaa gagcgaaatg gttccgaagc agcatctcgg
    14941 gcagcagctc ctcaattctc gcgcgtgacg ataacctacg gtcacttatc agattaatcg
    15001 agggcacaat tatgccggac gccggcattg cccggacctc gaccgagccg gccggcaccg
    15061 gcacgacggc gcctttacct ggtgacgatc tggttcacgc aatcggcagg caaccgcaat
    15121 catagggcct gaaagccgtt agcggagtgc aaagaaaatt gctgacaggc aacccatctt
    15181 ccccaagccg gagcgagtgc cttgggcagg caatccctgg gacgagctgc acatgagctt
    15241 gggggatcag caacgagagc aaccaaaaaa gcagggcacc cagcctgggt tgctgggtgc
    15301 cctttctggg gcctgtgctt ggtagggggc gtccggcaca ggcaaggtgc cgtgaaaacc
    15361 ggctcgcccc gacttggttc ccctgcaatt gcggctcgga gcccaagccg cgattgcgca
    15421 ttcagctcag ctccgtccgc gaccgcgctt cgccagctcg ccgtaaacct tgacctctgc
    15481 ctgcccgcgg aagctcggcc gtgacctgta gaccatctcg atccggcgaa gatagctgcg
    15541 ctcgccccgt agatccaccg ccagatgccc accggggcgg atgttccggt cgatcttgat
    15601 cgtctcggtg tagccgttga aatacacgat cttcagcgag ttcaggtgga gctcgttgtc
    15661 ctcgaccacg aagtgcagca tccggaaggc cctgttgcgg aaccagtttt ccgattgctt
    15721 gacgttgatg acatcatggt cggtcttgaa cccgaccttc tgctcgccga gcagcaacca
    15781 atccttgtcg ggctggaagc ggacgggccg aagcggccgg ggggccggct cgccgtagac
    15841 cttgatgacg gcctgcccgc ggaagctcgg ccgtgacctg tagaccatct cgatccggcg
    15901 aagatagctg cgctcgcccc gcaaatcgat cggcaattgc ccacccgcac ggatcagcct
    15961 gtcgaccttt atctcctccg cgtagccatt catgtagatg actcgcaccg ccatcaggtg
    16021 caagtcgttg cgctcggccg cgaagtgcag ggtccgaaag gcccgatcgc ggaaccaatc
    16081 ctctgatttg ccgacttcga tcgtgtcgcg atcggcaagg aaaccgacac ttttttcacc
    16141 aagcagaacc aaatccgcct ttcgctgcgc ttgcgcaggt aaagcaagca acatcaggca
    16201 gagagcgacc agtccgccaa cccgcagcat tatcgtctcc tcatgaggga ataccgccag
    16261 aaactgcagc cgatcatctc ggcagaacag aaggtaaacg ccggaaaaag ttgttgtctg
    16321 acagctttag ggccttccca tggcggagat cggtcgagtt tgtgtcggct gctaatcgct
    16381 tctgctggcg agcggcaagg cgcttcctcg cgtgaatgcg agaggcccaa gctgcatata
    16441 cttcgagacc cggctcagcg cctctttagg gaccggccgt gatacaacgc ccactcgatt
    16501 tcgctgcggg agatgccgat gtccttcagc atgtgatcat cgagctcagc caaatgatgc
    16561 aaggcggcgc gccgatcgcg ccatctcaca tatccttgcc aacatgagaa aaccgtcgtc
    16621 tgcagccgac cgagcaagct ccgccggcct ggcatatcca agaacgctct gtacgaggcg
    16681 gccattgttt tgctcctgtc atcccattgc tccgagatgc cggaaagtat ggttgacagc
    16741 ccctgacagc gtggcgtcag gaggtctgac acgctcctga cagcgcggtg tcaggagtga
    16801 tacgctaatg gctcggacgg ccaactcttg ggtggagcgg aaagcgtgcg ccgcgccgat
    16861 cgccttttcg agatcatcca gctgatgcgc cgcaaggccc ccgtgcgcgc ccgcgatctg
    16921 agcgaggcgc tcgaggtgtc cgagcgcacg atttaccgcg atatccagga cctgatcgcg
    16981 agcggcgtgc cgatcgaagg cgaggcgggc atcggctacg tgctcaaggc ggggttcgac
    17041 ttgccgccgc tgatgttcaa ggagcaggag atcgaggcgc tggtgctggg cgcgcgcatc
    17101 gtcgaatcct gggcggatgc cgagctcgcc gaagcggcct cgaacgccat tgccaagatc
    17161 gaggcggtga tcccggatcg ccttcgtgat ttcatggcca acaccgcgct ccttgccccc
    17221 gcgcacagct acatggagcc catcaccttc gatctcgccg aattgcgccg ggcgttgcgc
    17281 agccagctca aaatccattt ccggtatcgc gatttcatgg cgcgcgaaac aactcggacc
    17341 gtcaggccgc tgtcgctcgc ctatttcggg ccggtctggc tgcttgcggc ctggtgtgag
    17401 ctgcgggacg attttcgcac cttccgcctg gatcggatcg accagctcca gattctcaac
    17461 gaacggttcc gcatcgagca aggaaagagc ctgcacgatt tcctgaagcg tgatcaggcc
    17521 tggaaccgca ggtccgagac ggtcacgagc atggaaacgg ggaacgactg acgaacttta
    17581 ccatttcgaa tgagccgtcc ggagggcagc gatgaaagcg ccaaagaaga tcgatttccc
    17641 gccttggaat gtccgccggt tcctggagcc cggccctgtc gtgctcgtca gctccgcgta
    17701 tcgcgatcaa cgaaccatca tgaccatggg ctggcatatg atcatggaaa tgcagccggc
    17761 gctcgtcggc tgctacatct gggaccagaa cttcagccaa aagctcgttc gcggcagcaa
    17821 ggaatgcgtg atcaatctgc cgacttcgga tctcctggaa actgtcatca aaatcgggaa
    17881 ttgccatggg aacgaggtcg acaaattcaa tgaattcggc ctcacagccg ccgaagctga
    17941 aacggttggc gcaccgctca tcgcggaatg ctatgccaat ttcgaatgca agctcgtcga
    18001 ctcgagcctg atccgaaaat acaatctttt cgtcttcgag gtcacaaagg ctcacgtggc
    18061 gagatcaccg cgctatccgg aaacggtgca ttatcgcggc gaaggcgtat tcatgatctc
    18121 gggagagaac cgcagctatc gccggctctt caagccggaa atgctttgat caccggaacg
    18181 gcggctcgtc gaaggaacgc agcttgcgcg aatggagcgt ttcgcgccgc tcgcgcagca
    18241 ggtcgagcgc cttcagaccg atttcgaggt gctgcgctac ggcagcttcg tagaaggcgt
    18301 tcgccgcacc cggcagcttg atctccccgt gcagcggttt gtcggacacg cagagcagcg
    18361 tgccgtaggg cacgcgcagc cggtatccgt tggcggcgat ggtgccgctt tccatgtcga
    18421 cggcgatggc gcgcgaaaga ttgatgcggc gccgctcgtg cgaatagcgc agctcccagt
    18481 tgcggtcgac gtccgtgacg acagtgccgg tcctcagccg ccctttgagc gcgtcccctt
    18541 tttcgccggt gacgatggca gccgcgtcct gcagcgcgac ctgcacctcg gccagcgcgg
    18601 ggatagggat atccgtcggc agcagggcat cgaggatttg gtcctgccgc aggtatgcgt
    18661 gagcaaggac gtagtcgccg atctgctgtg actgccgcag gcctccgcaa tgcccgacca
    18721 tgagccagca gttcgggcgc agcacggcca ggtgatcggt gatgttcttc gcgttcgacg
    18781 ggccgacgcc gatgttcacc aacgtcaccc cctcgccgct cggggcggtc aggtgatagg
    18841 cgggcatctg gaaccggtgc caggggcttg cggcgataac accatcagcg tcgtccgggg
    18901 aatcgccccg gcggatttcg gcaccgcccg gcagcacgag ccgttcgtac ggcgtatttg
    18961 cttccgcgag ccgcgccaag ccccagcgga cgaactggtc gacatagcga tggtagttcg
    19021 tcagcagaat ccagtgctgc acgttccgcc agtcactgcc cgtataatgc acgatacggc
    19081 gcaaggagta atcgacccgc acggcgtcga acagagacag cgggcgcggc tcgccctccg
    19141 gcgcttgcca ctcaccgtcg gccacttcgt cgccgacgag ggacaagagc ggcgtcggga
    19201 aatggcgggc gagctcggag gcggtgatgc ccttgcggcc gatctcttcg acccgctcca
    19261 tcgtgtacgg gaacgggatc tcctgagtgc tgacgctgac ctcgatttcc gccccgtact
    19321 cgcccaccaa caagcgcagc tgctccaaga gatagttccg gaaatgccgc gggtgggtga
    19381 tcgtcgtctc gtagacgccc ggccgctgga acttggcata ggcccgccgg gtacgcggcg
    19441 ccgggtccat gccgtagtag atgacgcgca ggaggggata gcggaacgct gcccgctcct
    19501 tctcggtcgg cggctcgcgg gtctcgagat agcggtcgag ggcgcggacc agggccgtcg
    19561 acgcccggtc gtagagctcc tcgagacgat cgacggcggc ttccggagt
//
LOCUS       DASVDU010000024        11223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1714174, whole genome shotgun sequence.
ACCESSION   DASVDU010000024 DASVDU010000000
VERSION     DASVDU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714174"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1418
                     /locus_tag="VF226_01850"
     CDS             213..1418
                     /locus_tag="VF226_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7072757.1"
                     /translation="MLDLLQGIRVVSFNHFLLGPMGIQALGDLGADVITVETTEGAWQ
                     RHWSSGDIWSDGQGMLHMCANRNKRNVAIDLKSEKGREIAFRLIDTADVVSENFRPGV
                     FEKLGFGYEVLKKRKPNIIFASASGYGPDGPYREKPGQDLLAQALFGIMAITGQAHTG
                     PRPAGVSVVDHHGAALLAMGILAALVRRERTGKGCRVDASLMQAALDLQAESLVAWLN
                     AAEKPKSISAYRHVGGWYYAAPYGVYATQDGHLALSISPLPLIAEAIGEPKLASYTEK
                     DTWKKQDEISSLIADRLVTKTTAEWVKLMEPLKIWHAPVQGYDQIVDDPQVKHMQAFV
                     TVPGGGPTKAPVTLVNHPVRYDGQAAEVRLPPQRLGAQTAEVLGELGFTQGEIEALAQ
                     DGVVKLLDS"
     gene            complement(1528..2517)
                     /locus_tag="VF226_01855"
     CDS             complement(1528..2517)
                     /locus_tag="VF226_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7072758.1"
                     /translation="MAAVAGVSTASVSRVLNAPEKVSPELRARVEAAVEKLAYVRHGS
                     ARALAARRFQTIGAVVPTLGVATFAAGIDALQERLESQGFSLLVACAHYDPDVEARQI
                     RSLLEHGIDGLVLVGHQRRPEIYRRLDAAGIPYVCTYTLANQNGLCVGYDNGAASRRM
                     VDYLIQLGHRRFCLITTPTQNNDRIAARFTAAFETIASADLPTPQVVEAPYAVGEGRA
                     ALRSAIERDPEITAVVCTTDLHALGAVAEARVLGLPVPSRLSITGFDDLDIVAEIDPP
                     LTTVHVPSREIGLRVADMLIATILGRPTTPVIELSASVVVRGSTARAPDLGAK"
     gene            2754..4061
                     /locus_tag="VF226_01860"
     CDS             2754..4061
                     /locus_tag="VF226_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7072759.1"
                     /translation="MLEGIMRTSRRTFFAVTAAALAAGFSYGASAQQTEIKVGYMKHP
                     IQDASLDMMEKWAKAHNVKLTRVPMAYSVFMEKVTATLTSETDQFDIIWHNDDWGQLW
                     KKWVEPTDDIPGMENTDPWVLEAFLNDDKKPTAVPMAHTVGTFFYRTDLLKPEEVPTT
                     FDELVEVSKRLQKEGKVKWGYVGAMAMNNTWFTFWWATWSNKCDLLKPFFERDYEKLK
                     ANNFEPGIADPCSQEVMEFWWDAMNKHKISPPGMTAYTRNESNAIFMAGDAAFTLIDS
                     THYGEFNDPKRSKIAGKVGMAPFPVGPRGERPTSWNEVWAWAIPKGVPAERKKIAKQM
                     LAAMLADEAGQIEMWKKTGGPPPNTKLWPKLRAEDKDFDMLSKAVFDHKPLAHSAYYF
                     PEWPALHKAYSDVAIGALQGKREDIPQALKDGVEKLRRAAAGN"
     gene            4265..5119
                     /locus_tag="VF226_01865"
     CDS             4265..5119
                     /locus_tag="VF226_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7072760.1"
                     /translation="MLALIAPAVFALTLFQIVPILTGANASFRDWSLYDPQKTWIGLD
                     NYIYVLTDDDFLWTILPNTFGFMFASVACSLVLGLGIALLLNRRFPGRWLVQTIILLP
                     LMVAPVIAAIMTRWMFNDQFGIVNVVLESLGFSPIPWLTQKWTAFFVILITDVWLWTP
                     WFALLLLAGLQSLPPEPFEAAKIDAASAWRTFRYITLPMLRPVIVVCVVIRAIDAFRT
                     FDIVWTISAGGPARSTEVFSLYAYIEAFQFLNFGRGSAAAVIGALIIIVFAMVLYRIL
                     NRWVEVSK"
     gene            5116..5970
                     /locus_tag="VF226_01870"
     CDS             5116..5970
                     /locus_tag="VF226_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7072761.1"
                     /translation="MSVAEAITAPRPRSFLEALAPGGKRAFFVLVIIAVCLLFAFPVL
                     WLVLTSLRPGYAVYYVHRGTDFTIENFFEVLSQEIVVKAIFNSFVISTLATVLSLGIT
                     VTSGYMLSRFRGPIPSTWFGLIYVFRCVPYVSWVLPLYFVTQSVGIFDTYWGLLLPHV
                     AVHICFFSWIMKGFFDGIDPSMEYAAMIDGCSRWGAFWRVALPAAVPGMTALAILCWL
                     YTWNEFLFALILTAQNTPILTVVMAQFVHELGMEWHLMSATAVIALGPALIVTIFGQK
                     YVIRGLKV"
     gene            5974..7071
                     /gene="ugpC"
                     /locus_tag="VF226_01875"
     CDS             5974..7071
                     /gene="ugpC"
                     /locus_tag="VF226_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7072762.1"
                     /translation="MAEVILSKVRKSYGKVLAVKDFSLDIADQEFVVLVGPSGCGKST
                     TLRMIAGLEEITSGTISIGNRVVNDLQPKDRDIAMVFQNYALYQHMTVYDNLAFGLRN
                     RGTPESEIAEAIKHAAKVLSIEALLDRRPRQLSGGQQQRVALGRCIVRNPKVFLFDEP
                     LSNLDAQLRAQMRLELKELRQRVPTTSIFVTHDQVEAMTLGDRIVVMKDGLVQQVGTP
                     LELYRRPINQFVASFIGSPSMNFFEMRVSGEGDKITLKGDGLSLSLPPARFPGLARNG
                     SDKVTVGVRPQHLRLGRAENDDQVGFSGTLMVTEQLGDEQLLAIRIGGNDVRVAGVDP
                     ELALATGSTIEVATQMDNLHIFDGTSGSALR"
     gene            7098..7538
                     /locus_tag="VF226_01880"
     CDS             7098..7538
                     /locus_tag="VF226_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX7072763.1"
                     /translation="MSDFYVKVGDSVKFAKTVGETDVYMFAGITGDFSVNHVNEQYMA
                     RSKYGRRIAHGALLIGYMSTCSTMMIEKCNGTGGEETPVSLGYDKVRFLGPVFIGDTV
                     NLTYTITEIDPVKRRSYSEIKAENQRGELVGVARHILAWTKNKD"
     gene            7562..8323
                     /locus_tag="VF226_01885"
     CDS             7562..8323
                     /locus_tag="VF226_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072764.1"
                     /translation="MRLKDKVCIITGGGSGIGRAACVLFAKEGAKLAIADKRLDAARA
                     VASECKELGAEVIALEVDVSKSADAARMVDETVKTFGRLDVLLNNAGYGIAGTVLETD
                     EAAWDDLMAVNVRGVFLCTKYAIPAMKASGGGSIVNTASVVANVGIRNRAAYCASKGA
                     VAALTRAVAIDHVADSIRCNAIAPGTIDTPYFSEILSKSPDAAAVRKGLEERQLMNRL
                     GTPEEIAAGMLFLASDESSFATGSILTIDGGMTAQ"
     gene            8456..8869
                     /locus_tag="VF226_01890"
     CDS             8456..8869
                     /locus_tag="VF226_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEX7072765.1"
                     /translation="MKARWLWLVLGFLSVGCGLVGAVLPMVPTTPFLLLAAFAFARSS
                     PRLHRWLLSHRYFGPLITNWQQHGSIDRKAKFSAIGVMVPMLGLTWYWGAAPWLLAAQ
                     AIVLAVVAAWLLTRPDHHASGPDDGRVARSKRTST"
     gene            8997..9923
                     /locus_tag="VF226_01895"
     CDS             8997..9923
                     /locus_tag="VF226_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072766.1"
                     /translation="MEPIISLSNLSKTYASGFQALKGISLEIHRGEIFALLGPNGAGK
                     TTLIGIICGLVNRTAGEVTVGGYDTIKDYRAARSLIGLVPQELTTDAFESVWNTVSFS
                     RGLFGKPADPAYIEKVLKDLSLWDKKDNRILTLSGGMKRRVLIAKALAHEPRILFLDE
                     PTAGVDVELRKDLWDVVRGLRADGVTIILTTHYIEEAEEMADRIGVINKGELIIVEDK
                     ATLMRRLGKKQLILELHEPLDAIPESLAHHHLTLAADGHELVFTYDTQAQRTGITALL
                     RDLAEAGIRFRDLSTTQSSLEEIFVDLVRQGR"
     gene            9920..10681
                     /locus_tag="VF226_01900"
     CDS             9920..10681
                     /locus_tag="VF226_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072767.1"
                     /translation="MNLHAVRAIYVFEMSRMRRTLLQSIVSPVLTTSLYFIVFGAAIG
                     QRIPEIEGVSYGAFIVPGLTMLLLLTQSVMNASFGIYFPRFTGTIYEVLSAPISSMEI
                     VLGYVGAAATKSIILGIAVLVTASFFVPLQIAHPFWMVSFLLLTAVTFSLLGFIIGIW
                     ADGFEKLQFVPMLVITPLTFLGGTFYSIDVLPPFWQAVSLVNPIVYLVSGFRWSFHGV
                     ADVNVGISLGMTMVFLVACLIAIWRIFATGYRLKT"
     gene            complement(10756..>11223)
                     /gene="soxG"
                     /locus_tag="VF226_01905"
     CDS             complement(10756..>11223)
                     /gene="soxG"
                     /locus_tag="VF226_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:1901053 - sarcosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX7072768.1"
                     /translation="FSVRGDAEAAQKIGRAFGVSLPLEPWRSATNGTRAALWLGPDEW
                     LLFADEREADAITAQVAAAMGETPCSWVDISHRNAGFLLSGPHAADVLNAGCPLPLSL
                     AAFPVGKCTRTLFGKAEIVLWRTDETEFRIEVWRSFAAYVAAFIGEAAREHMV"
BASE COUNT         2154 a         3463 c         3369 g         2237 t
ORIGIN      
        1 gcgttggcgc tggccggcat caggccggaa ggagcgctgg cggaagccgc cgagtaagga
       61 agatatcagc ggcgatcagg taaacagggg gtggcgcagc tccaagccgc gcctccaccc
      121 gtttggcatt gcgccctgtt ctcctgcgat gccggatcga taaatcgatc gataaatcgt
      181 ctgattggta aagttaaaat tcgaggaaac gcatgctcga ccttctgcaa gggattcggg
      241 ttgtcagctt caaccacttc ctcctcggac cgatggggat acaggcgctc ggcgatctcg
      301 gcgccgatgt catcaccgtc gagacgacag agggcgcgtg gcagcggcac tggagcagcg
      361 gtgacatctg gtcggacggc cagggcatgc tgcatatgtg cgcgaaccgc aacaagcgaa
      421 acgtggcgat cgacctcaag tcggagaagg gcagagaaat cgcgtttcga ctcatcgata
      481 cggcagatgt ggtttcggag aatttccgtc ctggcgtgtt cgagaagctc ggcttcggat
      541 atgaggtcct gaagaagcgt aaacccaaca ttattttcgc ttcggcttca ggttacggtc
      601 cggacggtcc ctatcgcgaa aagccgggac aggacctgct ggcgcaagcc ctcttcggca
      661 tcatggccat taccggtcag gcccataccg gtccgcgccc ggcaggtgtt tccgtcgtcg
      721 atcaccacgg tgccgcgctg ctggcgatgg gcattcttgc agcgctcgtt cgccgggagc
      781 gcaccgggaa ggggtgccgg gtggatgcca gcctgatgca agcggcactc gaccttcagg
      841 ccgaatcgct cgtcgcgtgg ctcaatgcgg ccgaaaaacc gaaatcgatc agtgcctatc
      901 gccatgtcgg cggctggtac tatgcagcgc cgtatggcgt ttacgcgacg caggacggtc
      961 atctggcgct ttccatctcg ccgctgccgc tgatcgccga agcgatcggc gagccgaaac
     1021 tggcctccta caccgagaaa gacacctgga aaaagcagga cgagatcagc tcattgatcg
     1081 cggaccgcct cgtgaccaaa acgacggcgg aatgggtcaa gctcatggag ccgctcaaga
     1141 tttggcacgc tcccgttcaa ggctatgacc agatcgtcga cgatcctcag gtcaagcaca
     1201 tgcaggcctt cgtcacggta ccgggcggcg gccccacgaa agcgcccgtg acgctggtca
     1261 accatccggt tcgctacgac ggccaggctg ccgaagtgcg cctgccaccg cagcggctcg
     1321 gcgcgcagac ggccgaggtt ttgggagagc tgggcttcac ccagggcgaa atcgaggcac
     1381 tggcacagga cggcgtcgtc aagttgctgg acagctgaca aggtcctgga tctggcggtc
     1441 ttttgacgat tccgaagttc cgctccgggt ccggccgcgg atcgaagcgg atttcggaat
     1501 cggacgctat cgagagggaa ggttccgcta ctttgcgccg aggtccggcg cccgtgccgt
     1561 cgatccgcgc acgaccacgg aggcggaaag ctcgataacg ggcgtcgttg gccgcccgag
     1621 tatggtggca atcagcatgt cagcaacccg caatccgatc tcccgtgacg gcacatgcac
     1681 tgtcgtcaac ggcgggtcga tctcggccac gatgtcgaga tcgtcgaaac cggtgatcga
     1741 cagtcgcgac ggaaccggaa gccccagtac gcgcgcctcc gccacggctc cgagcgcgtg
     1801 caggtccgtc gtgcaaacca ccgccgtgat ttcgggatcg cgctcgatgg cgctcctcaa
     1861 tgctgcccga ccctcgccga cggcataagg agcttccacc acctgtggcg taggcaggtc
     1921 tgcagaagca attgtctcaa acgctgccgt gaagcgtgct gcgatgcggt cgttattttg
     1981 agttggcgtc gtgatgagac agaaccgccg gtgcccgagc tgaatgagat aatccaccat
     2041 gcggcggctg gcggcgccgt tatcgtaacc gacgcagagc ccgttctgat tggcgagcgt
     2101 ataggtgcac acataaggaa tgccggcggc atccaatcgg cggtagattt cggggcgcct
     2161 ctgatgtccg accagaacga ggccgtcaat gccgtgttcc agcaagctcc ggatctgccg
     2221 cgcctctacg tccggatcgt aatgcgcgca ggcgacgagt agggagaaac cttgactttc
     2281 cagcctctcc tgcagcgcgt ctattcctgc ggcaaacgtc gctacaccaa gggtcggcac
     2341 cacggcacca atggtctgga aacgccgcgc cgccagcgcc cgcgctgagc catggcggac
     2401 gtaggccagt ttctcgaccg ccgcctccac ccgcgccctc agttcggggc tgaccttctc
     2461 cggggcgttg agaactctcg aaacggatgc cgtcgacacg ccggcgacag ctgccacgtg
     2521 ggccatcgtc ctcggcgcag cgcgcgaggg cctacgacgg cgcgaagctt tcatgccttt
     2581 gaccggtttc tccaaacgcg aacgttccaa tgagtaaacc tcgacttgcg aaatgctcgc
     2641 gagccgaatg acgggttatc gtgtaccttg tcattgcggc ctccgcatgt tagcgcttac
     2701 acgagcgtct aaaataggac aagagacgtg tgttgaacca gaaaggagga aacatgctgg
     2761 aaggcatcat gcgaacaagc cgccgcacgt ttttcgctgt gacggccgcc gcgctcgctg
     2821 ccggtttctc gtacggcgcg tctgcgcagc agaccgaaat caaagtcggt tacatgaaac
     2881 accccatcca ggacgcatcc ctggatatga tggaaaaatg ggccaaagcg cacaacgtga
     2941 agctcacacg tgtgccgatg gcctacagcg tcttcatgga gaaggtcacg gcaaccctga
     3001 cctccgaaac cgatcaattc gacatcatct ggcacaacga tgactggggc cagctctgga
     3061 agaagtgggt cgagccgacc gatgacattc caggcatgga aaatacggat ccctgggtgc
     3121 tggaggcgtt tctcaatgac gacaagaaac ccacggccgt gcccatggcc cacacggtcg
     3181 gcacgttctt ctaccgcacc gatctcctga agccggaaga ggtgccgacg actttcgacg
     3241 agctcgtcga ggtgagcaag cgcctccaga aggaaggcaa ggtcaagtgg ggctatgtcg
     3301 gcgccatggc catgaacaac acttggttca ccttctggtg ggccacatgg tcgaacaagt
     3361 gcgaccttct caagccgttc ttcgagcgcg actacgagaa gctcaaggcc aacaacttcg
     3421 agcccggaat tgctgatcct tgcagccagg aggtaatgga attctggtgg gatgcgatga
     3481 acaagcacaa gatcagcccc ccaggcatga ctgcctacac ccgcaacgaa tcgaacgcca
     3541 ttttcatggc gggtgatgcg gccttcacgc tgatcgactc cacgcactac ggcgagttca
     3601 acgacccgaa gcgctccaag atcgccggca aggtcggcat ggctcctttc ccggtgggac
     3661 cacggggcga gcgcccgact tcatggaacg aggtctgggc ctgggccatt ccgaagggcg
     3721 tcccggctga gcgcaagaag atcgccaagc agatgcttgc agcgatgttg gccgacgagg
     3781 ccggtcagat cgagatgtgg aagaagaccg gcggcccgcc gccgaacacc aagctctggc
     3841 cgaagctgcg cgccgaggac aaggacttcg atatgctgtc gaaggccgta ttcgaccaca
     3901 agccgctggc ccactcggcc tattacttcc ccgagtggcc cgcgctgcac aaggcctatt
     3961 cggacgtggc gatcggcgct ctccagggca agcgcgagga tattccgcaa gccttgaagg
     4021 acggtgtcga gaagctccgt cgcgcggcgg ccggcaacta gtcgccacca ccgaccggct
     4081 gccttttcga gggaaaagcc aaacctcatc ccaggatagc gttttcgcca gtcctgggat
     4141 gagcctgatc tggtccaatg cgcgcgtacg ctctcaattg agcagctttt acgatatcaa
     4201 cgacaggcac ctgaccctga tggcttcgcg tcctttcctc gggctagacg aaaaaaagcg
     4261 attcatgctg gctctgatcg cgccagccgt cttcgcgctc accttgttcc agattgtgcc
     4321 catcctcacc ggcgcgaatg ccagctttcg cgactggtcg ctctacgacc ctcagaaaac
     4381 ctggatcggt ctcgataatt atatctatgt cctgacggat gacgatttcc tctggacgat
     4441 cctgcccaac accttcggct tcatgtttgc gagcgttgcc tgctcgctgg tcttgggtct
     4501 cggcatcgcc ctgttgctca atcgacgttt cccgggccga tggctggtgc aaacgatcat
     4561 attgctgccg ctgatggtgg ccccggtcat tgccgctatc atgacccggt ggatgttcaa
     4621 cgaccagttc ggcatcgtca acgtcgtctt ggaatcgctc ggcttctcac cgatcccatg
     4681 gctgacgcag aagtggaccg cattcttcgt cattctcatc acggatgtct ggctgtggac
     4741 gccatggttt gcattgctgt tgctcgccgg cctgcaaagc ctgccgcctg aaccattcga
     4801 ggcggccaag atcgatgccg cctcggcttg gcgcaccttc cgctatatta cgctgccaat
     4861 gctgcggccg gtgatcgtgg tctgtgtggt catccgcgcg atcgacgcgt ttcggacttt
     4921 cgacatcgtt tggacgattt ccgccggcgg cccggcgcgc tccactgagg tgttcagtct
     4981 ctatgcctat atcgaggcct ttcaattcct gaacttcggc cggggctctg ctgcagccgt
     5041 gatcggcgca ttgatcatca tcgtgttcgc aatggtgctc taccgcatcc tgaaccgctg
     5101 ggtggaggtg tcgaaatgag cgtggctgag gccatcaccg cgccgcggcc gcgcagcttt
     5161 ctcgaggcgc tggccccagg cggcaagcgc gcgttcttcg tgctggtaat catcgcggtt
     5221 tgcctgttgt tcgccttccc agtgctctgg ctggtactga cgtctttgcg cccaggctat
     5281 gccgtctact acgtgcatcg cgggaccgat ttcaccatcg agaacttctt cgaagtcctc
     5341 agtcaggaaa ttgtagtcaa ggcgatcttc aatagctttg taatttcgac actggccacg
     5401 gtcctctcgc tcggcatcac ggtaacgtcc ggctacatgc tctcgcgctt ccgcggcccg
     5461 atcccgagca cctggttcgg cctgatttat gttttccgct gcgtgcccta tgtctcctgg
     5521 gtgctgccgc tctacttcgt gacgcaatcc gtcggcattt tcgataccta ttggggactg
     5581 ctgctgccgc acgtggccgt gcacatctgc ttcttctcgt ggatcatgaa aggctttttc
     5641 gacggcatcg atccgtcgat ggagtatgcc gccatgatcg acggctgctc gcgctggggc
     5701 gcgttctggc gagtcgcact gccggccgcc gttcctggca tgaccgcgct ggcgatcctg
     5761 tgctggcttt atacctggaa cgaatttctg ttcgcgctca tcctcaccgc ccagaacacg
     5821 ccgatcctga ccgtcgtcat ggcccagttc gttcatgagc tcggaatgga gtggcacctg
     5881 atgagcgcca ccgcggtgat agctctcggc ccggcgctga tcgtcaccat tttcgggcag
     5941 aagtacgtca ttcgcggatt gaaggtctag atcatggccg aggtcattct cagcaaagtg
     6001 cgcaagtcat acggcaaggt ccttgccgtg aaagatttct cgctcgatat cgccgatcag
     6061 gaattcgtag tgctggtcgg cccatccggc tgcgggaagt cgacgacatt gcgcatgatc
     6121 gcgggactgg aggagatcac gagcggcacg atcagcatcg gcaatcgcgt cgtgaacgac
     6181 ctgcagccca aggaccgcga catcgcgatg gttttccaga attacgcgct ttaccagcac
     6241 atgaccgtgt acgacaatct cgcgttcggg ctgcgcaacc ggggcacacc ggaatccgag
     6301 atcgccgaag cgatcaagca cgcagcgaag gtattgtcga tcgaagcgct gctcgaccgc
     6361 cgtccgcgtc agctctcggg cgggcagcag cagcgggtcg cgctcggccg ctgcatcgtt
     6421 cgcaatccga aggtcttcct tttcgacgag ccgctttcaa atctcgacgc gcagctgaga
     6481 gcgcagatgc gcctggagct caaagagctc cgccagcggg tccccacgac ctccattttc
     6541 gttacacacg atcaggtcga ggcgatgact ctcggcgatc gcatcgtcgt catgaaggac
     6601 gggctggtgc agcaggtcgg cacgccgctc gaactctacc gccggccgat caatcagttc
     6661 gtggcgagct tcatcggctc accatccatg aacttcttcg agatgagagt ttccggcgaa
     6721 ggcgacaaga tcacgctcaa gggcgacgga ctgagccttt ctctaccgcc ggcacgtttc
     6781 cccggcctcg cgcgaaatgg ctcagacaag gtgacggtcg gtgtccgtcc gcagcacctg
     6841 cggctcggcc gtgctgaaaa cgacgaccag gtgggcttct cgggtacgct gatggtcacg
     6901 gagcagctcg gggatgagca gcttctcgcc attcgcattg gcggtaacga tgtccgcgtc
     6961 gccggcgtcg accccgagct cgcgctcgcg accggcagca ccatcgaggt cgcgacacaa
     7021 atggacaacc ttcacatctt cgacggcacc tccggttccg ctctgcgctg aaatcagctt
     7081 atcctgggag acttaaaatg agcgacttct acgtgaaggt cggcgactcg gtgaagttcg
     7141 ccaagaccgt gggcgagacc gacgtctata tgttcgccgg catcacgggc gacttctcgg
     7201 tcaaccatgt caatgagcag tacatggccc gatcgaagta cggcaggcgc atcgcccatg
     7261 gcgcactgct catcggctac atgtcgacct gctcgacgat gatgatcgag aagtgcaatg
     7321 gcaccggcgg cgaagaaaca ccggtctcgc tcgggtatga caaggttcgc ttcctcgggc
     7381 ctgtgtttat cggagacacg gtgaacctga cttataccat taccgaaatc gatccggtga
     7441 agcgccggtc ctatagcgaa ataaaggccg aaaaccagcg cggcgagctc gtcggcgtcg
     7501 cgcggcacat tctggcctgg accaagaaca aggactgact ggtcaggagc tgcggatcgt
     7561 catgcgcctc aaggacaaag tttgcatcat caccggtggc ggatcgggaa ttggccgagc
     7621 cgcctgcgtg ctgttcgcca aggagggcgc gaagctcgcc atagccgaca agcgcctcga
     7681 cgcggcccgc gctgtcgctt ctgagtgcaa ggagctcggc gcggaagtca ttgcgctgga
     7741 ggtggatgtc tcgaagtctg ccgatgccgc ccgcatggtc gacgaaacgg tcaagacgtt
     7801 cggccggctg gacgtcctcc tcaacaatgc cggctatggc atcgccggga ctgtgctgga
     7861 aaccgacgaa gcggcctggg acgacctgat ggccgtcaat gtccgcggcg tcttcctctg
     7921 caccaagtac gccatccccg cgatgaaggc gagcggcggc ggctcgatcg tgaatacggc
     7981 ctcggtcgtc gcaaacgtcg gcatccggaa tcgtgccgcc tattgcgctt ccaagggcgc
     8041 ggtggcggcg ctgacgcggg ccgtcgccat cgatcacgtt gcggacagca ttcgctgcaa
     8101 cgccattgct cccggaacga tcgacacgcc ctacttcagc gaaatcctca gcaagagccc
     8161 ggatgcggcc gctgtccgga aggggcttga ggagcgccag ctcatgaacc gcctcggcac
     8221 gcccgaggag atcgccgccg gcatgctgtt ccttgcaagc gacgagagca gcttcgccac
     8281 gggctcgatc ctgacgatcg acggaggcat gaccgcacag taggctgcaa ccacagccct
     8341 acgtccctcc tcccgggggt gtcgctcaac gactcttggt cacatcgaat cggtggccgc
     8401 atcgggccac tttagcgcca gcgcgtgtct gggaaggcca aggaggcgat cggccgtgaa
     8461 agcgcgctgg ctttggctgg tgctcgggtt tttatcggtg ggttgcggcc tcgtcggcgc
     8521 cgtcctcccg atggtgccga caaccccgtt cctgctgctg gcggcctttg cgtttgcacg
     8581 cagctcgcca cggttacacc gctggctgct ctctcaccgg tatttcgggc cgctcatcac
     8641 caactggcag cagcatggca gcatcgatcg caaggccaag ttttccgcga taggtgtcat
     8701 ggtgcccatg ctggggctga cctggtactg gggcgcagca ccctggctgc tggcggcgca
     8761 ggccatagtg ctcgcggtcg ttgcagcgtg gctcctcacc cggcctgatc atcacgcttc
     8821 agggcccgac gacggccgcg tggcccgttc gaaacgcacg agcacttgat ttccaggttg
     8881 tatcgggctt tttggcaatc cagccaccgg atcgccacac ttctcttgaa gtggtctcgc
     8941 ccctcgttcg atctattccg gcgagcccca aacaagatca tgagcacgtt caggccatgg
     9001 agcccatcat ctccctttcg aacctgtcga agacctatgc ctcgggcttt caggcgctga
     9061 aaggcatcag cctggagatc caccgcggcg aaatcttcgc cctgctcggc cccaatggcg
     9121 ccggcaagac gaccctgatc ggcatcatct gcgggctggt gaaccgcacc gcgggtgagg
     9181 tgaccgtcgg cggctacgat acaatcaagg attatcgggc cgctcggtcc ttgatcgggc
     9241 tcgttccgca ggagctgaca accgatgcat tcgaatcggt gtggaacacc gtcagcttca
     9301 gccgcggact gttcggcaag ccggcggatc ccgcctacat cgagaaggtc ctcaaggatc
     9361 tctccttgtg ggataagaaa gacaaccgca tcctgacatt gtcaggcggc atgaagcggc
     9421 gggtgctcat cgccaaggcc ctcgcgcatg agccgcgcat cctgttcctc gacgagccga
     9481 ccgcgggtgt cgacgtcgag ctgcgcaagg atctttggga cgtggtgcgc ggcctccggg
     9541 ccgatggagt caccatcatt ctcacgacgc actacatcga agaagccgag gagatggccg
     9601 atcgcatcgg cgtcatcaac aagggcgagc tgatcatcgt cgaggacaag gccaccctta
     9661 tgcgtcggct cggaaagaag cagctcattc tcgagttgca cgagccgctc gacgccatcc
     9721 cggagagcct tgcgcaccat catctgacgt tggccgccga tgggcatgag ctcgtcttca
     9781 cttacgacac gcaggcccag cgcactggca tcacggcttt gcttcgcgac cttgcggaag
     9841 cagggatccg cttccgcgac ctcagcacca cgcaaagctc gctcgaggaa atctttgtcg
     9901 atctcgtgag gcaaggccga tgaacttgca cgctgtccgg gcgatctacg ttttcgaaat
     9961 gtcccgcatg cggcgcacgc tgctgcagag catcgtctcg cccgttctca ccacgtcgct
    10021 ttatttcatc gttttcggcg cagcgatcgg ccagcgcatt cctgagatcg aaggcgtgag
    10081 ctacggcgcg ttcatcgtgc cggggctcac gatgctcctg cttctgacgc aaagcgtcat
    10141 gaatgcgtcg tttggtattt atttcccgcg tttcacgggg acgatttacg aggtgctttc
    10201 ggcgccgatt tcttcgatgg aaatcgttct cggctatgtc ggggcagcgg cgaccaaatc
    10261 gatcattctg ggaatcgccg ttctcgtgac ggcgagcttc ttcgtacccc tgcagatcgc
    10321 gcatccgttc tggatggtgt ctttcctgct tctgacggcc gtaaccttca gcctgctcgg
    10381 cttcattatc ggcatctggg cggacgggtt cgagaaactg cagttcgtgc cgatgctcgt
    10441 tatcacgcca ctgacgtttc tcggcggaac attttactca atcgatgtgc tgccgccgtt
    10501 ctggcaggcc gtcagcctag tcaaccccat cgtgtatctg gtcagcggtt tccgctggag
    10561 cttccacggg gtggccgatg tcaatgtcgg catcagcctc ggaatgacga tggtgtttct
    10621 cgtggcgtgc ctgatcgcca tctggcgcat cttcgcgacg ggttatcggc taaagacttg
    10681 aactcggtac aattcacagc cgagccgccg agccgggtgt ccacgggaaa tgtcgttgag
    10741 gccgatcatg gcctgtcaga ccatatgctc ccgtgccgct tcgccgatga acgctgccac
    10801 atacgctgcg aaggagcgcc agacctcgat tcgaaactcc gtttcatcag tccgccaaag
    10861 gacgatttct gcttttccga acagcgtgcg tgtgcacttg ccgaccggaa acgcagcgag
    10921 actgagcggc agcgggcagc cggcattgag cacgtcagcg gcatgaggac cgctgagaag
    10981 gaagccagca ttgcgatggg agatatcgac ccaagaacaa ggcgtctcgc ccattgccgc
    11041 tgcgacctgc gcagtgatgg catcggcttc gcgttcgtcg gcaaagagca gccattcatc
    11101 tggcccgagc cataaagccg cgcgagtgcc gtttgtggct gaccgccaag gctccagcgg
    11161 caaagatact ccgaacgccc ggccgatttt ttgggccgct tccgcatcgc cgcgaacgga
    11221 gaa
//
LOCUS       DASVDU010000025        28811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_174529, whole genome shotgun sequence.
ACCESSION   DASVDU010000025 DASVDU010000000
VERSION     DASVDU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28811
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174529"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VF226_01910"
     CDS             complement(<1..719)
                     /locus_tag="VF226_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7072769.1"
                     /translation="MEPTRRSDENIKTLSKVRRVLECFSTRRRSLSLAEICEETGYPK
                     STAHRLVSSMRVIGFLDQDRERDRYRLGLKLFEFGSIALANLELHREALPEIEQLRQT
                     TGHAVHLAVFDGYQAVVIHRSESLSNTVTPVHLIENAPIHCTSVGKAILAFQPSEIIE
                     KVIAVGLEKYTDATIVTEGLLKEELGKIRSQGYSIDDGEHRPGLRCIGAPIRDQFGNV
                     AASVSISAPAWQIPLDDTEKLS"
     gene            866..1768
                     /locus_tag="VF226_01915"
     CDS             866..1768
                     /locus_tag="VF226_01915"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7072770.1"
                     /translation="MGENEVDVRGSLTGVLPPVVTPFDKAGNIDFGCLREQIDFIVEA
                     GCTGIVIGGSTGEGHTLNSEDFCELMEAAHEALNGRGSLLAGLIVNSTKEAIDRLKRI
                     EGMKVDALQVTPVHYLFKPGPDATVSHFKKIAEHTPVPLLIYNVIQWNYLSVDLMLRI
                     MDEVPGVIGMKQSNGDLKSVSDLLLRKKSENVVLTGIDGLLYPAFMLGVDGAITALTA
                     AVPGLVVKLWKAVQNGDHRQAREIHFRLAKLWNSMTHDNLPACTKYVQHRQGLKLSFP
                     RAPMEEVSAEQKRSMDAALDEIGL"
     gene            complement(2156..2914)
                     /locus_tag="VF226_01920"
     CDS             complement(2156..2914)
                     /locus_tag="VF226_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072771.1"
                     /translation="MDLELTDKVALVTGASAGLGRAIAKMLAAEGCRLIVVARRQERL
                     VELAQEIVADGGAEPFVVVQDLMEDDAVEKIRLAVKERYDRLDILVNNAGGSRPLTGL
                     GTPEEWDEGMRLNFTVGRDLAHAFLPLMQKNRFGRIINITGGDEPVALNAAVPPNGAV
                     HIWAKALSRVVGRDGITVNCIPPGRIHSEQIDQRLLPTPEAQATWVKENCPVGYIGEP
                     EDLAVLVAFLSSPKARYITGQVIYVDGGARRFSH"
     gene            complement(2930..3640)
                     /locus_tag="VF226_01925"
     CDS             complement(2930..3640)
                     /locus_tag="VF226_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072772.1"
                     /translation="MPLEVKGLSAGYGDVSVLREVSFTVPDDGVVALLGANGAGKTTS
                     LNAVSGLETQVTSGSIKLDGEEISTMPAHLRVAAGIAHIPQGRQLFPFMSVRENLELG
                     SYLSRTRDKEKQTLEWLLTIFPRLKERLSQSAGSLSGGEQQMCAIARGLMSQPRYLLL
                     DEPSLGLAPIMVKQVVGVIETIARQGIGILLVEQNVSLALKQVRYAYVLENSRISIEG
                     PAAELAQDDRIRRAYLGL"
     gene            complement(3630..4346)
                     /locus_tag="VF226_01930"
     CDS             complement(3630..4346)
                     /locus_tag="VF226_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072773.1"
                     /translation="MDIRNVSRRFRGLVAVDDVSFTVAPGELVGLIGPNGAGKTTLFN
                     VLSGHLPPSGGQVLLEGHDISRLTATHCARRGIGRTFQVVKPLTTLTVHENVTVGALL
                     HHPLAVAKEKAEAIIRRVGMASLMDQPASSLTLESRKRLELARALAIDPKILLLDEIL
                     AGLNTYEINESIELIRSFSKSDGLAVIMIEHNLHAVMSLADKVVVLDYGQKIAEGMPS
                     EVVRDPKVIAAYLGDDQDAA"
     gene            complement(4361..5245)
                     /locus_tag="VF226_01935"
     CDS             complement(4361..5245)
                     /locus_tag="VF226_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072774.1"
                     /translation="MSAFLNDYYVNIAVMTCLWAALSGAWNLMAGYGGLVSLGHAAFF
                     GLGAYVTAIVYTKFGLSPWFGLLAAMLVTTGIAVALSWPCFRLRGAFFSLATLVFPIA
                     MEIVANNWVEYTRGSSGIAIPFNPGIENFIFRSRLAYLVAAFILMVIVYLITRWMHRG
                     KLGLYLIAIRDDPATAASMGIDAVRVKLLVTAISGALTAAGGFFYAQYILFLDPPSMF
                     SINVSIQIALLSIVGGLGTPAGPIVGSLLMTPVDGVLSQFLGGGPRLLIYGLVLLTVV
                     LLAPRGIVGSIRQLRRAR"
     gene            complement(5242..6129)
                     /locus_tag="VF226_01940"
     CDS             complement(5242..6129)
                     /locus_tag="VF226_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072775.1"
                     /translation="MISILPQLIVSGILIGGIYALLSIGLTLIFGVLRIVNFAQGEFI
                     MLAMFGAFWLHQLWGIDPYLSIFIIAPVIFVLGLATERIVIKPILHAPHAMQIFATFG
                     ISVILQNLALTLWGPDYRSIQTAYSTQSFIVGTVAISASSLYGFLIALAMAGALIGFL
                     QFSRHGKALRALVQNRYAASLMGIDTERLNRLAFGVGVACAAVAGCVLTPIYYTFPTV
                     GIDLIITAFVVVVLGGLGSVTGAIIGGLIIGITQTLTGFFISVEFKDLIALILFIAIL
                     LIRPQGLLGRAGMEEVGMK"
     gene            complement(6208..7458)
                     /locus_tag="VF226_01945"
     CDS             complement(6208..7458)
                     /locus_tag="VF226_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072776.1"
                     /translation="MKQLLAGLLIVAAVATGASAQEEEDWRIGALYPLTGNLALLGNE
                     NLNGARIAVDMINEKGGVDGHKIVLVTGDASTPDKAQSEAERLASLENLDVITGTYSS
                     GLSYAASQVIERHGGLYWETGGISDGLTQRGFKGYFRVVFTASSNGQLAATIAKEIIA
                     RKLEKQPSELTVLIVHEDSDYGTSVAKAAEAKATELGFNVADRLPYKATTTDLSSLIL
                     RMKASGANIVIASSYANDALLIQRQMKQLGVNVDAFIGTGGIYGLPSFAEGLRSAVNG
                     IFDTEGSAAINTAALTDDAAALLAEFKERFEKAYGSVPSYVGTQGFVGTYVLLDRVAR
                     KAGSLDPEALKQAALSLDIEEGGTPLGWGVKFAGEGDPMQGQNLRAFPVAQQWQHGKL
                     VVVAPDKFKTADATLVPLPTWSER"
     gene            complement(7455..8174)
                     /locus_tag="VF226_01950"
     CDS             complement(7455..8174)
                     /locus_tag="VF226_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEX7072777.1"
                     /translation="MSDAVERPWDLFLTPADRAVLARGRFGHRMGFGKRPAIVAIDCQ
                     RYMVGERGVEDDRYPSSCGSMGWQAIDRIATILEVGRKAGVPIFLTRFALDPSGSDVG
                     VYRHKRAFMLRDDWCIEGTPGAELLPEVGPREGDIVFVKKKPSGFYGTPLLASLIERR
                     VDTVIVVGGATSNCVRATVFDAASNNYRAIVPADAVFDRLPISHAINLFDMDRQFADV
                     TTSAAVVEELEKLSGKKGEMS"
     gene            complement(8171..9118)
                     /locus_tag="VF226_01955"
     CDS             complement(8171..9118)
                     /locus_tag="VF226_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HEX7072778.1"
                     /translation="MAAPDGSSNLIRDFVGYADRRPDAKWPNGARIAVQFAINYEEGS
                     EYSIGDGDGRTELGLAETPGGRVPEGRRDIAFETMYEYGSRVGIWRLMRLFGERGVPA
                     TIFGCAVAMERNPRFVEAFMKAGYDLCCHGWRWEEHFRLSEEEERDHIGRAVELITRL
                     TGERPLGWYCRYGPSENTRRLLVEEGGFLYDSDSYNDELPYWTVVNGRSHLVVPYSMD
                     ANDGKFALAAGYSSQDDFEAYLKGTFDQLYEEGATTPALMSVGLHPRITGRPARARAL
                     GRFLDYILSFDGVWVCRRLDVARHWAALHPPPNEKKADR"
     gene            complement(9105..10328)
                     /locus_tag="VF226_01960"
     CDS             complement(9105..10328)
                     /locus_tag="VF226_01960"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7072779.1"
                     /translation="MSAAFRESAGQAERAMPLEGLKVIDCATLFAGPLIGTLMGDFGA
                     DVIKIEHPNGDPLRNMGYKKDGHGLWWKVAARNKRCIALNLKEPEDAKIFKALVADAD
                     VLVENFRTGTLESWGLGWEELSAINPRLIMVRVTGFGQSGPYRRRAGFGTLAEAMSGF
                     AFITGEPDGPPTLPPFGLADGVAAYFGCFASMFAIYERDVRGSGKGQFIDLAIYEPIF
                     ALLGPQPTVYDQLGIIQTRNGNRSANNAPRNAYKTKDGRWVALSAASPSIVRRVLDLT
                     GGPGTADDPRFQTNLDRVRHVEDIDRIVGGWIARHDLTDVIEAFERVEAAIAPIYDIE
                     QIFHDPQFRERRSIVAVEDEDLGPIVMQNVFPTLSRTPGRIRFPGGRIDQHREEILAE
                     LADRIGHGGGSDGGT"
     gene            complement(10325..11179)
                     /locus_tag="VF226_01965"
     CDS             complement(10325..11179)
                     /locus_tag="VF226_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7072780.1"
                     /translation="MRSKLFVPGSRPELFEKAIASGADAVAFDLEDAVVPERKAQARA
                     VVASFLAERRAGAPLLIVRINPIGSPYFQKDIEALVLPGLDVLNLPKIESGDEVRDVS
                     TLIADLETQRGLQHRIGILANIETPRAVRLAAEIATSDRRLVGLQLGFGDLFEPFGID
                     RSDPVAAAAVKLSVRLAAAEGNLPLYDSAFPAVEDMAGFRAEAESARRLGLAGKSCIH
                     PSQVATANEVFFPTAKEIDVAKQIVAAADEKFSQGIGAFMLNGVMVDEPYVARARALL
                     AIAAGGAS"
     gene            11331..12263
                     /locus_tag="VF226_01970"
     CDS             11331..12263
                     /locus_tag="VF226_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7072781.1"
                     /translation="MDVRRLRYFCRVVESGSMSRAAISLNVAQPALSKSIQTLEQELE
                     TELFQRTSKGTVPTEAGERLYEHAQIIFTQIDRAKVEIRESTARPTGHVVVGMPHSII
                     SRLGMPLLKEVTRRFPSIRIELAQEHSHVLAARLRSGRIDIAVSAAQRPSSELTSTPL
                     LIEELFLVTPRDGKGNNGSAISFAEASRLSYILPSLSNGLRATVESQFRARSLPLDVK
                     YEIDAIGLIPQCVEAGLGATILPGGCIDFAMEGKLTAWRFEENGCHRTLVITHSADAT
                     PTPASSAIIRMIVELTHKLVRDQGWPGAKLIATE"
     gene            complement(12594..13754)
                     /locus_tag="VF226_01975"
     CDS             complement(12594..13754)
                     /locus_tag="VF226_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7072782.1"
                     /translation="MPTSSTSPQLPDFAALPFETEAMLKGLRRWVEQESPTFEKRAVD
                     RMMDLASRELAMLGAEIQRIPGQMGYGDCVKGIFRRNGDDRPGILILGHMDTVHPLGT
                     LETLSWRQEGGRCYGPGILDMKGGNYLAVEAIRQLRRIGFATPLPVTVLFTSDEEVGS
                     PSTRDLIEAEAARHKYVLVPEPARRNGGVVTGRYAIARFWLTTRGRPSHAGLRLSEGV
                     SAIREMAQQILAIEAMTDEETGYSVGVVRGGRWVNCVSTSCQAEVLVSATTDEKLAEA
                     IRRMETLQPVGSGTGVEVRTGVIRPVWTSNDGSWQLYEHARALAQSLGFDIPHQSSGG
                     GSDGNFTGAMGVPTLDGLGVRGDGPHTLEEHIVSESLPERGRLMAALLATLN"
     gene            13882..14676
                     /locus_tag="VF226_01980"
     CDS             13882..14676
                     /locus_tag="VF226_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX7072783.1"
                     /translation="MVKKPTAAEPTGTRRKQDRLLVESLARGLHVLEAFAQAHGPMSL
                     SQIAAAARIDKSAAQRLTHTLVKLGYLEKAPGGLVPGRRILDRSFDYLRTTPLVNRAV
                     PVLADLRKAAQERVDLSLFDDLTMLYLVRMQSKRETFFAHLIGRRVPTFCTSGGRAVL
                     SHLPHDQVLDILERSDRRKLTPRTTTDIGEILERIAQVRETGYSLAIEEVLVGEVAVA
                     AAVLDPSGAPIGAIHVAGSLAEWKPEDFMKRMGPLATAAANALRGS"
     gene            14761..15588
                     /locus_tag="VF226_01985"
     CDS             14761..15588
                     /locus_tag="VF226_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072784.1"
                     /translation="MGAKLQCARIAVRYEIVEAPIAYPMMNGTSTIREVAHTAPLLSV
                     AGLAYDYAGVSAVADCSFEVPRGRIVALIGGNGAGKTTSVKMIAGALRPRRGNILFDG
                     ESITGLPAHNAVEKGLCLVPEGRLVFQQLTVRENLLVSGRVARARSRLQENLDRVFDL
                     FPRLGERQSQNAGSLSGGEQQMLAIGRGLMTMPRLLILDEPSLGLSPILVTTIFSLVR
                     RLNEEGMSVLLVEQNVHQTLAIADYAYVLEKGRVVQSGEGRGLLNDPKVKEAYLGLA"
     gene            15621..16844
                     /locus_tag="VF226_01990"
     CDS             15621..16844
                     /locus_tag="VF226_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7072785.1"
                     /translation="MHRREFLKTGTALALAGAMPRIARAQQSKIVFGGSVPMTGVAAE
                     TGLNVLRGYECAVKFINEEMGGVDVGGQKYTLELNLFDDASDPARATTLIQRQVDEGI
                     GFFLGSFGSNIVLPTCAITEAAGRIMVQAGGGSDLIFTQGRKRVFGIFPRASRQFVSS
                     VNMFKELEPAVKTVSIMYTNDPFSKFQAEGAKASIEEAGMQVLDFIALPAQVSDVTNV
                     LTTIRQNTPDVLVCTTHDDASILIARQMISTNTNVPMLYQTLGPQTAAYKEALGNYAN
                     GVVCQAYWSERAPFKGEYFGSAQDFAKYYRANFDRELTYHMASGAACIICYLQAARNA
                     GTLDLDPVRDALAALDFQCFYGHIRFAPEGDGAADLLGPLLIQRQKGEIEVISPADAA
                     TAKPIYPAPDWADRA"
     gene            16947..18092
                     /locus_tag="VF226_01995"
     CDS             16947..18092
                     /locus_tag="VF226_01995"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX7072786.1"
                     /translation="MTSLPLLELGPDPRERGRIHGSTLAAAVRDNVETYLARFEAGGV
                     SRRTVLEQSGQWLAFMQGDNPEYAEEMAALAEASGISLTEIAMLNARYEITYSIFGAE
                     AKAANSRPEQEGCTSFGLMPEATASGRTLLCQNWDWLEKVRGRTFVQRVRRSSTPGEG
                     KPDFVGFSEAGIVGSKMGVNAAGIGLCVNGLVTPRDGANGIRKPFHVRCKEILDAWRF
                     DQALTPVVQTDRTASSNFLIGHADGEIINIEATPDHCSYIYPVDGLVVHANHLVKEGR
                     VGSQMERISSNTLYRGPRLERLLRAKLGRIDFDVLRECMSDHFSAPAAICRHADTALP
                     EPKRVITVAAIVLDLKERVLYATDGQPCQSDFQAFPLSTETSISNAA"
     gene            18103..18933
                     /locus_tag="VF226_02000"
     CDS             18103..18933
                     /locus_tag="VF226_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX7072787.1"
                     /translation="MNDMTPFPWPNGARCAVMLTFDFDAETLWLSRDPDNARRPGVLS
                     QGAYGARTGVPKILELLKEEDLKATFFVPGWTAEKHVERVEAIVAGGHEVGHHGYLHE
                     WIDPDYPDKEREALEKGLEALKKTVGVKPAGYRSPAGETSTNMISLLQEYGFLYDSSL
                     MDQVSPYRHVLQNGGRGPVELPWHWSLDDAPYALFAIKSPRAIMTNEHILSIWKQEFT
                     EIYRWGGLVNIVTHPQVIGRPSRLAMLREFIAFVRRSPGVWFATGREVAEAWAAKETA
                     "
     gene            18950..19828
                     /locus_tag="VF226_02005"
     CDS             18950..19828
                     /locus_tag="VF226_02005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072788.1"
                     /translation="MILFLQTTLNGLVLGGIYSLAAVGFSLIFGVTGIVNLTHGIFVV
                     AGAYGAMLLYREVGLDPLLAVIPIGAVLFAVGYIYQRTIIHWAISRASLVASLVVTFG
                     VAMVARNLLQLGFGPDVQTITPSYSFTSLALGPLRIDLVRLLALGASLVLLTALALVL
                     SRMHFGRAVRATAQQPLAAALSGLNVNNLHGLTFGVSAAMAGASGAIIGIVQPFSAAS
                     EVAWTLNAFIVVVLGGIGSPAGALLGGLLLGLINAYTAQYVGPALTTAMMFLVLVIML
                     VVRPQGLLGNSFGESR"
     gene            19825..20805
                     /locus_tag="VF226_02010"
     CDS             19825..20805
                     /locus_tag="VF226_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072789.1"
                     /translation="MNRFLAAFAGLAAVAVIALLPLWLGPFYTRVIQFFFFSAALAIA
                     WNILGGFAGYWSFGHTAFIGIGAFAAAHFVMQFGAFGTGPVSMVLPVLVAALVCAVFA
                     ALLAYPVLRLRGIYFAIVMLGVAQVVSELVNNVNWFQGGVGVYLPSPVPSGMAPEDFY
                     YYVFAALLLVTFLLSVAIRRTRFAYGLLAIREDEDTAMMLGVPTERFKIAAFVLSSVL
                     VGVLGAVYAYSVGYFTTYTVFRLDFSLNMIVFCLIGGIGTLFGPIVGTAVMLFLTQVL
                     LSRFLDIHLLITGLIVVTITLVAPGGILGILRRRPRRAKLAAEPEAERSA"
     gene            20802..21551
                     /locus_tag="VF226_02015"
     CDS             20802..21551
                     /locus_tag="VF226_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072790.1"
                     /translation="MSEIILSGRGVTKRFRGLTAIESVDFEIPVGAIYGLIGPNGAGK
                     STLFNLITGYYDPSAGEISFRGKSLAGVPTFRRNQLGIARAFQISKPFPALTVRENVA
                     VGAMFGSPDRTDVEHVTTEALRIAGIEDLADRTAEGLTVGALRKLEIARAIATRPKLL
                     LADEPCAGLNPTETAEMVSCLRNIRGHGVTVWLVEHDMKAVTSICDRIIVIDAGRKIA
                     EGTPQQVVSDPKVIAAYLGTPVAEETAPGPG"
     gene            21577..23616
                     /locus_tag="VF226_02020"
     CDS             21577..23616
                     /locus_tag="VF226_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7072791.1"
                     /translation="MTELRGISASGNGANRIGVEIGGTFTDLVWVAPDGQLRTGKTPS
                     TPQAIHEAVLKAIDEVGAPLASVEQVTHGSTVATNALLTRRGAHAGLVTTAGFRDVLI
                     VGRGDRDNNIYDMQYRRPAPPIRRSMIREVPERIGPHGEVLLPLDEEAAWREISELID
                     QGVEAIAICLLHSYCNPAHERRLAEMIRERAPHVAVAASHEVSPEFREYERSMTTVVN
                     AFVGPVVERYVDRLDAALRDRGYGGVLQIMQSNGGSMPAVSAGANAVRMLLSGPAAGV
                     RAAMWFAARNGIQNAITLDMGGTSTDVAIAPGLTPGIVPELRVDGLPVRTASIDMATI
                     GAGGGSIASIDRGGFLAVGPESAGALPGPACYGRGGEWATVTDAQVVAGLLRPDHFLG
                     GRITLSTDKARAALDALGMSGGAEAAADAVLRLVNSSMADAVRLVSTRRGIDPRDFAI
                     VAYGGGGPLHAAMVAEELGVRQVLVPWSPGLASAFGLLIADTVIDVARSSLHRLSGET
                     LDETRLSELKESATEAARRNGLTQGSYEVAIGLDMRYAGQAFELTVWPGTGAAAAAEL
                     RGLFEAEHERRYGYARATLAVEVVGYRIRVIRRSGGAMSTPVPAGGGPAPISLPATIG
                     GQRMNVSFMARDSLFPGACIDGPAVVEEPTSTTVVPPGWRLQCLVTGDLLLRDAR"
     gene            23613..25337
                     /locus_tag="VF226_02025"
     CDS             23613..25337
                     /locus_tag="VF226_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7072792.1"
                     /translation="MTRDKTRLIILARGLQAAADEMAANLIRSAFSAVVREARDCSTA
                     LLDAQGRVVAQADMIPMQTAALSGSFQAAAEQLDLSGVRPGQFVLMNDPYSGGQHLND
                     IILFSPIFFEDALIGWSGSTAHHLDIGGGSAGVNTSATDLIQEGIVIPPLLLDIERDW
                     HGGMVERLIFANIRTPEIGLGDMDAQFAANHVGAERVMEMARRFGPEVVADAMREVLD
                     YSERRMRAAIEEIPDGSWRGEAFLDGDGRDPQGPPIRIVATVTIKGDEAILDFTGTDP
                     QVRTMFNSPLASSIAGGVTALRSILGDRDMPANDGCNRPLTLIIPEGTLLNPRPGAPV
                     RARATACCRALDAVHAALGQVLPERTPAQGANSTTGFFLTHARPDGGVDIHLDVLGGG
                     WGAAKGYDAIHATDHVLSSCRLTPTESIEQLSPHLLIESFGLIQDSWGAGAFNGGMGL
                     FRRFRVLKEDVTLSLYSDRFRLPPKGREGGCDGTCASLFVLRDGERIELGATSTFALK
                     RGDLVEIRLPGGGGWGNPRERDRELVVRDLEDGLISRDFARDRYGLDVDRWAPVEDRS
                     ADTKLTTG"
     gene            25340..26530
                     /locus_tag="VF226_02030"
     CDS             25340..26530
                     /locus_tag="VF226_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7072793.1"
                     /translation="MRNQASGHELLHPADIDVAPLMQGIIDWVEMETPSDRPDLIDRL
                     LDHVERSFDNLPVTVRRIPGRDGCGGQLVLTYAPAGCEGKPALIMGHVDTVWAAGTLS
                     ERPVRREDDRLYGPGIFDMKAGSYLATETMRRIAAAGLVPPRPITVFLNSDEEIGSPT
                     SRDTIEELAQKAAFVLVPEPAFEAPGTVITARKGWARFSITAHGRSSHAGGNLADGRS
                     AIREIARHILDIEGLNDTEEKATFNVGTVKGGTLLNVVPAEARIEIDMRAEDMATAER
                     LVNEMLARRPFDPDIRIEVEGGLNRPTFERSAKVARLYEATCALAEELGLPMAETSRG
                     GVSDGNFAAALGLPVLDGLGCNGAGAHAVDEHILISTIAPRHALIHGMFMSRRFQEMA
                     LEEA"
     gene            26816..27910
                     /locus_tag="VF226_02035"
     CDS             26816..27910
                     /locus_tag="VF226_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX7072794.1"
                     /translation="MPGLFSKFECGPLELPNRIVVSPMCQYSAENGCAGDWHLQHLMT
                     LAMSGAGLVSFEATAVEPAGRITHGDLGLFSDENERSIKRVLEAARSVAPAGTRFAIQ
                     LGHAGRKASTQRPWEGGGPLAAEENPWQTVAPSAIPFAEGWHVPEALDEAGLRRIRDA
                     FVESARRAVRLGFDVIDLHLAHGYLLHQFHSPLANQRTDAWGGDGERRLAFPLSVARA
                     VREAVPESVLVSARITGSDWREDGLTPEDAVAMATALKAAGIGMVCVTSGGVAPAPIK
                     AGPGYQVPFAEKVKRESGLPTQAVGMIMTAEHADSVIAEGKADLVALARPLLDDPRWP
                     WQAARKLGAQVDVPPQYRRAVPPAWPGRPA"
BASE COUNT         5395 a         8953 c         8951 g         5512 t
ORIGIN      
        1 gacagcttct cggtgtcatc gagagggatc tgccatgcgg gagcgctgat gctgacgctg
       61 gctgcgacat tgccgaactg gtcgcgaata ggggcaccga tgcatcgcaa ccccggacga
      121 tgctccccgt catctattga gtagccttgg gagcggattt ttccgagttc ctctttaaga
      181 aggccctcgg tcacaatcgt ggcgtcagtg tacttttcca gtcctacggc gataaccttt
      241 tcgataatct cgctgggctg aaatgcaaga attgcctttc cgacgcttgt gcagtgaatc
      301 ggtgcgttct cgatcaagtg taccggtgtc accgtattcg acagtgattc agaacggtgg
      361 atcacgacag cttggtaacc gtcaaagacc gccaagtgaa cagcgtggcc ggtggtttgc
      421 ctcagctgct caatttcagg gagcgcttcg cgatgcagct cgagattcgc aagagcaatg
      481 ctcccgaatt cgaacaattt caatcccaag cgatacctgt cgcgttcgcg gtcctggtcg
      541 agaaacccga tgacccgcat ggacgagaca aggcgatgcg cggtgctttt cgggtatcct
      601 gtctcttcgc aaatttccgc cagtgaaagc gaacgtcggc gcgtggagaa gcactccagc
      661 actctgcgaa ccttgctcag tgttttgatg ttctcgtccg aacgacgggt gggttccata
      721 gctttcctgt acagctcgag ctttcagcaa gctctcggga ttagttgcgc cattcatcgc
      781 gctgttgacg tatcgccatg ttttccccta acttgccctt tcacggaatt caattccgga
      841 atgcgggacg gttgcggagg tcgaaatggg cgagaatgaa gttgatgtga ggggatcttt
      901 gacaggcgtt ctgccgcccg ttgtgacacc gttcgataag gccggaaata tcgacttcgg
      961 ttgtttgcgc gagcagatcg atttcatagt ggaagccgga tgtacgggca ttgtcatcgg
     1021 cggaagtacc ggggaagggc atacgctcaa tagtgaagac ttctgcgaac ttatggaagc
     1081 cgctcatgaa gctctgaacg ggcgcggtag ccttcttgct ggcttgatcg tcaatagtac
     1141 aaaggaagcc attgatcggt tgaagcggat tgaagggatg aaggtggatg cgctccaagt
     1201 gacgcctgtc cattatcttt tcaagcctgg tcccgatgcc accgtctcgc atttcaaaaa
     1261 gatagctgag catactccgg ttccgctcct gatctacaac gtgatccaat ggaattacct
     1321 gtcagtcgac ctgatgctca ggataatgga tgaggttcct ggcgtaatcg gaatgaagca
     1381 gagcaacggg gatcttaaat ccgtctcaga tttgcttctg cggaagaagt ccgagaatgt
     1441 tgtgctgaca ggaatagacg gccttctgta tccggccttt atgctcggtg ttgacggagc
     1501 gatcacggcg ctgacggcag cggtgcccgg actagttgtc aagctttgga aagctgtgca
     1561 gaacggagac cataggcaag cgcgcgaaat acatttccgc cttgcgaaac tttggaattc
     1621 catgacgcat gacaacctgc ctgcttgcac gaaatacgta cagcacaggc aggggctcaa
     1681 gctatcattt ccccgggcac cgatggagga ggtgtcagcg gagcagaaac gctcgatgga
     1741 tgccgctctt gacgagattg gcctctgacc ttcgcgttct tgaacccgtg gcacagcgca
     1801 aataaggcct gatcgtcgaa atcaacaaca ggtccgaggt cgacctacaa cgcttcgtaa
     1861 gcctgcttca gcttggagtt tgaggctccc cggttgtcac ccgtcgtcgc gtgtgccgca
     1921 gcctcaggca caggctccga caaggcccct tgcggatccc cggctccggc aacagcctga
     1981 aggttgccct gtagcatcgg gacattgccc gatcaaaccg tggcgatcgg ggtgaggggc
     2041 gagccgaccg agcccggcag ccccccagtg gcggggcggt cagaaagaag gccgtgcggt
     2101 cattctcgtg cagccagcca caagcacgcg catgcgggcg caacttcaac ctgcttcagt
     2161 gcgaaaaccg cctggcgccg ccgtcgacgt atatcacctg acccgtgatg tagcgggctt
     2221 tcggtgagct gaggaacgcg acgagaacag caaggtcttc cggctcgccg atatagccga
     2281 cgggacagtt ctccttcacc cacgtggcct gagcctcagg ggtcggcagc aggcgctggt
     2341 cgatctgctc ggaatggatc cgtcccggcg gaatgcaatt gacggtgatg ccgtcgcggc
     2401 cgaccacgcg ggatagtgcc ttggcccaga tgtgcactgc tccgttgggc ggcaccgccg
     2461 cgttgagggc taccggctcg tcgccgccgg tgatgttgat gatgcggccg aaccggtttt
     2521 tttgcatcag cggcaggaag gcgtgggcga gatcgcgccc gacggtgaag ttcaggcgca
     2581 ttccctcgtc ccattcctcc ggcgtgccaa ggccggtcaa cggccgcgaa cccccggcgt
     2641 tgttgacgag gatatctagg cggtcatatc gttccttaac ggccagcctg atcttctcca
     2701 ccgcgtcatc ttccatgagg tcctggacca cgacaaatgg ctcggcgcct ccgtccgcca
     2761 cgatttcctg tgccagttcg accaaccgct cctgccgtcg cgccacgaca atcaggcggc
     2821 agccttcagc agccagcatt tttgcgattg cacggccaag acctgccgaa gcgccggtta
     2881 ccagggcgac tttgtccgtc agttccagat ccaccgtcct cgtgtccttc taaagtccaa
     2941 gataggcgcg gcggatgcga tcgtcctgcg ccaattccgc cgcaggccct tcgatggaaa
     3001 tgcgcgaatt ctccagcaca taggcataac gcacctgctt cagcgccagt gaaacgttct
     3061 gctcgaccag aagaatcccg attccctgac gggcgatggt ctcaatcact ccgaccacct
     3121 gcttgaccat gatcggtgcc agaccgagcg acggttcatc cagcagaaga tagcgcggct
     3181 gggacatgag tccgcgggca atggcgcaca tctgctgttc gccgccggac agcgaacccg
     3241 ctgactgcga caggcgctcc ttaaggcgcg ggaatatggt aagcagccac tccagcgttt
     3301 gcttttcctt gtcgcgcgta cgggacagat aggagccgag ctccaaattc tcccgcacgc
     3361 tcatgaacgg aaagagctgc cgcccttgag gaatgtgggc gatgcccgct gcaacccgta
     3421 gatgcgcagg cattgtggag atctcctccc cgtccagctt gatggaaccg gacgttacct
     3481 gtgtctcgag gccggagaca gcattcaggc tggtcgtctt tcctgcacca ttggcaccca
     3541 gaagggcgac gacgccgtcg tctggcacag tgaaactgac ttcacgaagg acggagacat
     3601 cgccgtagcc ggcggagaga cctttgactt caagcggcat cctggtcgtc tccgaggtag
     3661 gcggcgatca cctttgggtc acgcaccacc tcactgggca tgccctccgc gatcttctgt
     3721 ccgtaatcga ggacaaccac cttgtcggca agcgacatca ccgcgtggag gttgtgctct
     3781 atcatgatga cagcaagtcc gtcggacttg gaaaagctgc ggatcagttc aatgctctcg
     3841 ttgatctcat aggtgttgag accggcgaga atttcgtcaa gcaggaggat cttcgggtcg
     3901 atcgcaagtg cacgcgcaag ttccagccgc ttgcgcgatt caagggtcag gctgcttgcg
     3961 ggctggtcca tcaagctcgc cattccgacg cgcctgatga tcgcctcggc cttttccttc
     4021 gcgacggcga gcggatgatg cagaagcgct cccacggtaa cattctcatg cacggtcagc
     4081 gtcgtgagag gcttcaccac ctggaacgtg cggccgatcc ctcttcgtgc gcagtgcgtt
     4141 gcagtaagcc gcgagatgtc atgtccttcg agcaggacct gccctccgct tggtggcaga
     4201 tggccgctca agacattgaa cagcgtcgtc ttaccggccc cattcggccc tatcaggcca
     4261 accagttcgc cgggcgcgac ggtgaacgag acatcgtcga cggcaaccag accgcgaaaa
     4321 cgccgcgaca cgtttctgat atccatgagg acatctttgg tcaccgcgcc ctcctcaact
     4381 gccgaatgct gccgacaata ccacgcgggg cgagcagaac gacggtgagc aggacgaggc
     4441 cgtagatcag cagccgcggc ccgccgccga ggaactggct caacacgccg tccaccggag
     4501 tcatcagaag tgagccgaca atcgggcctg caggagtgcc gagcccgccc acgatgctca
     4561 gcagtgcgat ctggatcgac acgttgatcg agaacatgct cggaggatcg aggaacaata
     4621 tgtattgggc gtagaagaag ccgcccgctg ccgtgagcgc gccgctgatg gccgtcacga
     4681 gaagcttcac gcggaccgca tcgatcccca tgcttgcagc ggtcgccgga tcatcgcgaa
     4741 tggcgatcag gtagaggccg agcttgccac ggtgcatcca ccgcgtaatc agatatacga
     4801 tcaccatcag gatgaacgcc gctaccagat aggcaaggcg ggagcggaag atgaaattct
     4861 cgattcccgg attgaagggg atcgcgatgc cggaagaccc tcgcgtatat tccacccagt
     4921 tgttggcgac gatctccatg gcgatcggaa acacgagagt tgccagcgaa aaaaaggcac
     4981 cacgcaggcg aaaacacggc cagctcagcg ccacagcaat tccggtcgta accagcatgg
     5041 ccgccaggag gccgaaccag ggcgacagcc cgaatttggt gtagacgatg gccgtcacat
     5101 aggcgcccag gccgaagaag gctgcatggc cgagcgagac gagcccccca tatccggcca
     5161 tcaggttcca ggcgccggac agcgctgccc agaggcaggt catcacggcg atgtttacgt
     5221 agtagtcgtt gagaaacgcg ctcatttcat ccccacttct tccatacccg cgcgtcccag
     5281 cagtccctgc ggtcgaatga gcaaaatcgc gatgaagaga atgagtgcga tcaggtcctt
     5341 gaactctacc gagatgaaga aaccggtgag tgtctgcgta atgccgatga tcaatccgcc
     5401 gatgatggcg ccggtgacgc tccccagccc gccgagcacc accaccacga atgccgtgat
     5461 gatgagatcg atgccgaccg tcgggaacgt atagtagatc ggcgtcagca cacagcccgc
     5521 aaccgctgcg caagcaacgc ctaccccgaa ggcaagccgg ttgagccgct cggtatcgat
     5581 ccccatgagg ctcgccgcat agcggttctg caccagggcc ctgagcgcct ttccgtgccg
     5641 ggagaactgc agaaagccga tcagcgcacc tgccatcgca agtgcgatca gaaagccgta
     5701 gagcgagctt gccgaaattg ccacggtccc gacgataaac gattgggtgg agtaggcggt
     5761 ctggatcgag cgatagtcag gcccccaaag cgtcagcgcc aggttctgca ggatcaccga
     5821 gattccgaaa gtggcgaata tctgcatcgc gtgaggggca tgaagtatcg gcttgatgac
     5881 gatgcgctcc gtcgccaatc cgagaacgaa gatcaccggt gctatgatga agatcgaaag
     5941 gtaagggtct atcccccaga gctgatgcag ccagaacgcc ccgaacatcg cgagcatgat
     6001 gaattcgccc tgcgcgaaat tgacgatgcg cagcacgccg aaaatgagtg tcagcccgat
     6061 cgaaagcagc gcgtagatgc caccgatcag aataccggaa acgataagtt gcggcaggat
     6121 cgagatcatg tggagcgcgc actcctgtcg agaatccgga gggcagccat tgccgaggcc
     6181 gccctccgaa tgcagttact gcctgagtca gcgctcggac caggtcggca gcggaacgag
     6241 cgtggcgtcg gcagttttga acttgtccgg cgccacaaca acgagctttc catgctgcca
     6301 ctgctgagcc accgggaagg cacgcaggtt ctgcccctgc atgggatcgc cttcgccggc
     6361 gaatttcacg ccccagccaa ggggcgtgcc gccttcctct atgtccagtg acaatgctgc
     6421 ctgcttcaac gcctctggat cgagcgagcc ggcctttcgc gcgactcgat cgagcaggac
     6481 gtaggtaccg acaaagcctt gggtaccgac ataggaaggc acgctgccat acgctttctc
     6541 gaaacgctcc ttgaactccg ccaacagggc ggccgcgtcg tcggtgagtg ctgccgtgtt
     6601 gatcgccgca gagccttcgg tatcgaaaat cccgttgacg gcgctgcgga gcccttcggc
     6661 aaagctcggt agaccataga tgccgccggt gccgatgaat gcgtcgacat tgacgccgag
     6721 ctgcttcatc tggcgctgga tgagcagcgc atcattggca taggacgagg caatgacgat
     6781 gttcgccccg cttgccttca tgcgcaggat cagtgacgag aggtcggtcg tggttgcttt
     6841 gtaaggaagg cgatcggcga cattgaaacc cagctccgtc gccttggctt ctgcagcctt
     6901 cgccacagag gtgccgtagt cggaatcttc atgaacgatc agcacggtca gttcggaagg
     6961 ctgcttttcc agcttccgtg caataatctc ctttgcaatc gtggcagcaa gctggccatt
     7021 cgagctggcc gtaaacacga cgcggaaata gcctttgaac ccgcgctgcg tcagcccgtc
     7081 cgaaatgccg ccggtctccc aataaaggcc gccatggcgc tcgatcacct gactcgccgc
     7141 gtaggaaagg ccgcttgaat aggtaccggt tatgacatca agattttcca gcgacgccag
     7201 gcgctcggct tccgactgcg ccttgtccgg cgtcgaggca tcgcctgtga cgagcacgat
     7261 cttgtgcccg tcgacaccac ctttttcatt gatcatgtcg acggcaattc gggcaccatt
     7321 gaggttctcg ttgccaagaa gcgcaaggtt tccggtgagg ggataaagcg ccccgatgcg
     7381 ccagtcctct tcctcctgtg cggacgcgcc ggtggcgacc gcagcgacga tcagcagtcc
     7441 tgcgagcagt tgtttcatga catttcccct ttttttccgg acagtttttc aagctcctcc
     7501 acgactgccg cactcgtcgt gacgtcggcg aattgccgat ccatgtcgaa caggtttatg
     7561 gcgtgggaaa tcggtaacct gtcgaaaacc gcgtcggcgg gtacgatcgc ccgatagttg
     7621 ttcgaggctg catcgaagac cgtggctcgc acgcaattgc tcgtcgcgcc gccgaccacg
     7681 atgacggtgt cgaccctccg ttctatgagg ctggctagga gtggcgtgcc gtagaagccg
     7741 ctcggcttct tcttcacgaa tacgatatca ccttccctgg gcccgacctc aggaagaagc
     7801 tccgcaccag gcgtgccttc gatgcaccag tcatcacgca gcatgaaggc gcgtttgtgg
     7861 cggtagacgc ccacgtccga gccgcttggg tcgagcgcga agcgtgtgag aaagatcgga
     7921 acgccggcct tccggcccac ctcgagaatg gtggcaatac gatcgatcgc ttgccagccc
     7981 atcgacccgc aggaagacgg atagcgatcg tcctcgaccc cgcgttcgcc gaccatgtaa
     8041 cgctggcaat cgattgccac gatcgccggg cgcttgccga agcccatgcg atggccgaag
     8101 cggccgcggg caagcacggc cctgtccgcc ggggtcagga acaaatccca cggtctttcc
     8161 acagcgtcgc tcaccgatcg gccttcttct cgttgggtgg tggatgaaga gcggcccaat
     8221 ggcgtgcgac atccaacctg cgacaaaccc agacgccatc gaagctcagg atatagtcga
     8281 ggaagcggcc caatgcgcgc gcccttgcgg gccgacctgt aatgcgcgga tgcaggccca
     8341 cggacatcag ggccggcgtt gtcgcaccct cctcataaag ctgatcgaag gtccccttga
     8401 gataggcttc gaagtcatcc tgcgaggaat accccgctgc cagcgcaaat tttccgtcat
     8461 tcgcatccat ggagtacggg accacgagat gcgagcggcc attgacgacg gtccagtagg
     8521 gcaactcgtc gttgtaggaa tcggaatcgt agagaaatcc gccttcttcc accaggaggc
     8581 ggcgcgtgtt ctcgctcggg ccgtagcggc aataccagcc gagtggtctt tcgcccgtca
     8641 gccgtgtgat caactcgact gcgcggccga tatgatcgcg ctcttcttcc tccgaaagcc
     8701 ggaaatgctc ctcccagcgc cagccgtggc agcagagatc gtaaccggcc ttcataaagg
     8761 cttccacgaa acgcggattg cgttccatcg cgactgcgca gccgaagatg gtcgccggta
     8821 caccacgctc gccgaacagg cgcatcagac gccagatgcc gacgcgactg ccatattcgt
     8881 acatggtctc gaaggctatg tcgcgccgcc cttccggaac gcggccgcct ggcgtctcgg
     8941 cgagacccag ttcggtgcgt ccgtcgccat cgccgatcga atattcggag ccctcctcgt
     9001 aattgatggc gaactgtacg gcgatccgcg cgccattcgg ccatttggca tcgggcctgc
     9061 gatcggcata gccgacaaaa tcgcgtatca ggttggacga accgtcaggt gccgccatca
     9121 cttcctcctc catggccgat cctgtctgcc agttccgcga gaatctcctc gcgatgctgg
     9181 tcgatccgcc cgccggggaa gcgaatccgg cccggcgtcc gcgacaatgt cggaaagacg
     9241 ttctgcatca cgattggtcc gagatcctcg tcctcgacgg caacgatgga gcgccgttct
     9301 ctgaactggg gatcatggaa gatttgctcg atatcgtaga tcggggcgat ggccgcctcg
     9361 acgcgttcga aggcttcaat gacgtccgtg agatcgtgac gcgcgatcca gccgcccaca
     9421 atgcggtcga tgtcctccac gtggcgtaca cggtcaaggt tggtctgaaa tcgcggatcg
     9481 tccgccgttc ccggcccccc cgtcagatca agtactcgtc gcacaatgct tggtgaggct
     9541 gcagaaagag ccacccagcg cccgtctttg gtcttgtagg cgttgcgcgg cgcattgttg
     9601 gccgaccgat tgccgttacg cgtctggatg atgccaagtt ggtcatagac cgtcggctgc
     9661 ggtccgagaa gcgcgaaaat cggttcgtaa atggcgaggt ctatgaactg cccctttcct
     9721 gatccgcgca catcgcgttc gtagatcgcg aacatcgacg cgaagcagcc gaaataagcc
     9781 gccaccccat ccgcaagacc gaagggcggc agagtgggcg gtccgtcggg ttcgccggtt
     9841 atgaatgcga agcctgacat cgcttctgcc agcgtgccga agccggcacg gcgacggtat
     9901 gggccgctct gaccgaagcc ggtgacgcgc accatgatca gccgagggtt gattgccgaa
     9961 agctcttccc aaccaagccc ccagctttcc agcgtgccgg tacggaaatt ttcgaccagc
    10021 acatcggcgt ccgccaccag cgccttgaag atcttggcgt cttccggctc cttcaggttg
    10081 agggcgatgc aacgcttgtt gcgagcggcg actttccacc agagcccatg cccgtccttc
    10141 ttgtaaccca tattgcggag cgggtccccg ttcgggtgct cgatcttgat aacgtcggcg
    10201 ccaaaatcgc ccatgagcgt gccgatgagc ggtcctgcga acagggtcgc gcagtcgatc
    10261 accttcagcc cttcaagcgg cattgctcgc tcggcttgcc cggccgattc cctgaaagcc
    10321 gcgctcacga tgcacctcct gctgcaatgg ccagcaaggc gcgggcccgc gccacatagg
    10381 gctcgtcgac catgacaccg ttgagcatga aagctcctat gccctgcgaa aatttctcgt
    10441 cggcggccgc tacgatctgc ttggcaacgt cgatctcttt cgctgtcgga aagaagactt
    10501 cgttcgccgt tgcgacctgc gagggatgaa tgcagctctt gccggcaagc ccgagtcgcc
    10561 gcgccgattc cgcctctgct cgaaaacccg ccatgtcctc aaccgccgga aaggcgctgt
    10621 cgtagagtgg aagattacct tccgcggcgg ccagccggac cgaaagcttg actgcggcgg
    10681 cggctacggg gtcgctgcga tctatcccga acggctcgaa gagatcgccg aaaccgagtt
    10741 gcaggccgac gagacgtctg tcgctcgtcg caatttccgc cgccagcctg acggcacgcg
    10801 gtgtttcaat attggcgagg atgccgatgc ggtgttggag tccgcgctgt gtctcaaggt
    10861 ccgcaatcag cgtggagaca tctctcacct catcgccgct ttcaatcttg ggcaggttga
    10921 gcacgtcgag cccaggcagg accagcgcct cgatatcctt ttggaagtac gggcttccga
    10981 tcgggttgat acgaacgatc agcagtggag cgccagcccg gcgttcggcc aggaaggagg
    11041 ccacgaccgc cctggcctgc gccttcctct cgggcaccac ggcatcctcc agatcgaagg
    11101 caaccgcatc cgcgccggag gcaatcgcct tctcgaacaa ttcgggccga gacccgggaa
    11161 cgaacaactt gctgcgcacc ggcgcccttc ctgaaaatga gacatgccgc ctgacgcagg
    11221 cgaaacttca gaaaggttag accctgggcg acaaggccga caaatatcgt ttttcttaag
    11281 ggatattcct gaaaggaatt gctcgtatga tgggataggg aggacggccc atggatgtgc
    11341 gaaggctgcg ttatttctgc cgcgtcgtgg aatctggcag catgagccga gcggccatta
    11401 gcctgaacgt cgcccagccg gcgctgagca aaagcattca gactctcgag caggagcttg
    11461 aaactgaact ctttcagcga accagcaaag gaacggttcc gacggaggct ggcgaacggc
    11521 tatacgagca cgcccaaatc atcttcacgc agatcgaccg cgccaaggtg gagataaggg
    11581 aatcgacggc gcgcccgacc ggccatgtcg tcgtaggaat gccccatagc atcatttcac
    11641 ggctggggat gccccttctc aaggaggtca cccgccgctt tccgtccatc aggatcgaac
    11701 tggcgcagga acatagccat gttctggctg cccgcctccg ttctggccgc atcgacattg
    11761 cggtttcggc agcgcagcgc ccttcatcgg agctgacatc gaccccattg ctgatagagg
    11821 aattgtttct ggtcacgccg cgagacggca agggaaacaa cggatcggcc atcagcttcg
    11881 cggaagcctc gcgcctctcc tacattcttc cctccttgag caacggtctc agggcgacag
    11941 ttgagagcca gttcagggcg aggtcgctcc cgctagatgt gaaatacgag atcgatgcaa
    12001 tcgggctcat cccgcaatgt gtggaggccg gtctcggcgc tacgatcctc cccggcggct
    12061 gcatagactt tgcaatggag ggaaagctga cggcgtggcg tttcgaggaa aatggctgcc
    12121 atcgaacgct ggtgatcacc cattccgcgg atgcgacccc tacgccggca agcagtgcaa
    12181 tcatccggat gattgtcgaa ctcacccaca aactggtgcg ggatcagggt tggccgggcg
    12241 ccaagcttat cgccaccgag tagtgcttgg agcggttctg acgtcggccg taccgaaaga
    12301 gggattcaaa tgctggctga ggaggaggcc atgcttctcg ttttacgaaa gacctcatgt
    12361 ggcccggagc gatgagcctt gccttgtgcc gcagcttcgc aacctcgcac gcgcggtgat
    12421 ggctgctggc gcagacctct ggccttcgcc gggatcaagc gtcggatgcg cgcagtccag
    12481 aagcgaaccg ttgaggagac acgatgccat gtcggcggcc tcgtcccccc agctcacggc
    12541 atgccgcagc tttctccccg gcacagggcg ctgtcgtcaa aatccggacg gctctaattg
    12601 agcgttgcca ggagggctgc catcaggcgc ccgcgctccg gcaggctttc gctgacaata
    12661 tgctcctcca gggtgtgggg cccatcgcca cgcacgccga ggccgtccag cgtcggaacg
    12721 cccatcgcac cagtgaaatt gccgtccgat ccgccgccgg aactctggtg cggaatgtcg
    12781 aagccgaggc tctgcgcaag ggcgcgggca tgttcgtaaa gttgccatga cccgtcgttg
    12841 ctggtccaga ccgggcgaat gacccccgtc cgcacttcga cgcctgtgcc cgatcccacc
    12901 ggctgcagcg tttccatgcg ccggatagcc tcggccagct tctcgtccgt cgtggccgag
    12961 acgagcacct ctgcctggca gctggtggaa acgcagttca cccagcgtcc ccctctgacg
    13021 acaccgacgc tgtagccggt ctcctcatcc gtcatcgcct cgatcgcgag aatctgctgc
    13081 gccatctcgc ggatggctga cacgccctcg ctgagcctca ggcccgcatg gctcggacgg
    13141 ccgcgtgtgg tgagccagaa gcgtgcaata gcgtagcggc ctgtcaccac gccgccgttg
    13201 cgccgggccg gctcgggcac taggacatat ttgtgccgcg cggcctccgc ctctatcagg
    13261 tcacgggtgc tcgggctgcc gacctcttcg tcgctggtga acagaaccgt gaccggcaaa
    13321 ggagtggcaa agccgatgcg ccgcaattgc cgtatggcct cgacggcgag gtagttccct
    13381 cccttcatgt ccaggatgcc cggcccgtag cagcgtccgc cttcctgccg ccagctcagc
    13441 gtctccagcg tgccgagcgg atggacagta tccatatggc cgaggatcag tatgccgggc
    13501 cggtcgtcgc catttcgcct gaagatgcct ttgacgcagt ctccgtagcc catctgaccg
    13561 ggtatgcgct gaatttccgc gccgagcatc gccagttcgc gggatgcgag atccatcatc
    13621 ctgtcgactg cacgcttttc aaacgtgggg ctttcctgct cgacccatcg gcgaaggccc
    13681 ttgagcatcg cttcggtttc gaaaggaagc gcggcaaaat caggaagctg cgggctcgtg
    13741 gagctagtgg gcatagcttg cgtatcgtcc tgcgatatga tgaaatcgct gcattataag
    13801 gctattgcgg gcagcgtcaa attctgccac cagttgcacg acgccaaacg cacaccctgg
    13861 aacatggaac gcaccgggcc tatggtgaag aagccgaccg ccgccgagcc aaccgggaca
    13921 aggcgcaagc aggacaggct gcttgtggaa tccctcgcaa ggggcttgca tgtgcttgaa
    13981 gccttcgctc aggcgcacgg tccaatgtcg ctgtcgcaga tcgctgcggc agcaaggatc
    14041 gacaagagcg ccgcccagag gctcacgcat acgcttgtga agcttggata tctggagaag
    14101 gctccgggcg gcttggtgcc gggtcgccgc atccttgacc gttcgttcga ctacctgcgc
    14161 acgacaccgc tggtgaaccg cgctgttccc gttctcgccg acctcagaaa ggcggcgcag
    14221 gaacgtgtcg atctcagcct cttcgatgat ttgacgatgc tctacctcgt ccgcatgcag
    14281 agcaagcgcg aaaccttctt cgcgcatctt atcggccggc gcgttcccac cttctgcacg
    14341 tccggcggcc gcgcggtgct cagccatttg cctcacgacc aggtgctcga catcctggaa
    14401 cggtccgacc gtcgaaagct cacgccgcgc acgacgacag atattggtga aatcctcgaa
    14461 cggatcgcgc aggtgcgcga aacaggctac tctctggcga tcgaggaggt gttggtcggt
    14521 gaggtggccg tcgcagcggc ggtcctcgac ccatcgggcg cgcccatcgg cgcgatccat
    14581 gtggccggtt cgctggccga atggaagccg gaggacttca tgaaacgaat ggggccgctt
    14641 gccaccgccg ctgccaacgc gctgcgcggg tcctagatcc ccgcacggcc tcgccggacc
    14701 gctctgcaac gaaccgcttt gatggcaggt tcgccggcag cttgcttgac aagggtgaat
    14761 atgggtgcaa agctacagtg tgctcgtatc gcagtgcgat acgagatcgt cgaagcgcct
    14821 atcgcctatc ctatgatgaa cggaaccagc acgatcaggg aagttgcgca cacggctcca
    14881 ctcctcagcg ttgctgggct cgcgtacgac tacgcaggcg tcagcgccgt agccgactgc
    14941 agctttgaag tgccgcgggg gcgcatcgtt gcgctgatcg gcggtaacgg cgcgggaaag
    15001 actaccagcg tcaagatgat agccggtgcc ttgcgtccga ggcggggaaa cattctcttc
    15061 gacggggagt cgatcaccgg gctgcccgcg cacaacgccg tcgagaaggg tctgtgtctg
    15121 gtgccagaag gccggcttgt gtttcagcag ctcacggtgc gcgaaaatct gctcgtcagt
    15181 ggaagggtgg cgcgcgctcg cagccggctg caggagaacc tggatcgcgt tttcgacctg
    15241 tttccccgtc tgggcgagcg ccagtcgcag aatgccggtt cgctttccgg cggcgagcag
    15301 cagatgctgg caattggccg cggcctgatg accatgccgc gtctgctgat cctggacgag
    15361 ccgtcgctcg ggctcagccc cattctggtc actaccatct tttcgctggt gcgcaggctc
    15421 aacgaggagg gcatgagcgt ccttctcgtc gagcagaacg tgcaccagac gctggcaatc
    15481 gccgactatg cctatgtgct cgagaaggga cgcgtggtgc agagcggcga gggtcggggc
    15541 ctcttgaacg acccgaaagt gaaggaagcc tatctgggtc tggcttaggc tggccagccg
    15601 ccgaaatggg aggaaaggac atgcaccgac gcgaatttct gaaaaccgga accgccttgg
    15661 ccctagccgg cgcgatgccg cgcatcgcca gggcgcagca aagcaagatt gtcttcggcg
    15721 gctcagtgcc catgaccggt gtggcggccg agacagggct caatgttctc cgcggctatg
    15781 aatgcgccgt caagttcatc aatgaggaaa tgggcggcgt ggatgtcggt ggccagaaat
    15841 acacgctgga gctcaatctc ttcgacgacg cgagcgatcc tgctcgcgcc accacgctca
    15901 tccagcgtca ggtggacgag ggtatcggct tcttcctcgg ctcgttcggc agcaacatcg
    15961 tgcttccgac ctgtgccatc accgaagctg ccggtcgcat catggtccag gcaggtggcg
    16021 gctccgacct gatcttcacc cagggccgga agcgcgtctt cggtatattc ccgcgcgcct
    16081 cgcggcagtt cgtgtcgtcc gtcaacatgt tcaaggaact tgagccggcc gtgaaaactg
    16141 tcagcatcat gtacaccaac gacccgttct caaagttcca ggccgagggc gccaaggcct
    16201 cgatcgagga agccggcatg caggtgctgg acttcatcgc gcttccggcg caggtcagtg
    16261 acgtcaccaa cgtgctgacg accatccggc agaatacccc ggacgtgctg gtctgcacca
    16321 cgcatgacga tgcctcgatc ctcatcgcgc ggcagatgat ctccacaaat accaatgtgc
    16381 cgatgctcta ccagacgctg ggcccgcaga cggccgccta caaggaggcg ctgggcaact
    16441 acgccaacgg cgtggtctgc caagcatact ggtccgagcg ggcgcccttc aaaggcgagt
    16501 acttcgggtc ggctcaggat tttgccaaat actaccgcgc gaatttcgac cgcgaactca
    16561 cctaccacat ggccagcggc gcggcctgca tcatctgcta tctgcaggcg gccaggaacg
    16621 cgggaacgct ggatcttgat ccggtgcgcg atgcgctcgc cgcgctcgac ttccagtgct
    16681 tttacggaca catccgtttc gcaccggaag gcgacggcgc cgcggacctg ctcgggccgc
    16741 tcctgatcca gcggcagaag ggcgagatcg aggtcatttc gccggccgac gcggccacgg
    16801 ccaaaccgat ctatcccgcg ccggactggg cagatcgggc ctgaggacgg tccgagccgt
    16861 tcgtggagcg gtgtcagttt ccgctcaggt tgggcccggc ttcggttggg cccatccgat
    16921 ccaccgtcga tgaagggata cgcgccatga catcacttcc cctgctcgaa ctgggaccag
    16981 atcctcgtga acgcggccgc attcatggtt ccacgctagc agcggcggtc cgcgacaatg
    17041 tcgagaccta tctggcccgg ttcgaggccg gcggggtcag ccgcagaacg gtgctcgagc
    17101 agagtgggca gtggctggct ttcatgcagg gcgacaatcc cgaatatgcg gaggagatgg
    17161 ccgctctggc cgaggcgtcg ggcatctcgc tgaccgagat cgcgatgctc aacgcgcggt
    17221 acgagatcac atattcgatc ttcggcgcgg aagcgaaggc tgccaactcc cgccctgaac
    17281 aggaaggctg tacatccttc ggtctgatgc cggaggcgac ggccagcgga cgtacactgc
    17341 tgtgccagaa ctgggactgg ctggagaagg tgaggggccg cacattcgtg cagcgcgttc
    17401 gccgctcctc gacgccgggt gaaggcaagc cggatttcgt gggcttcagc gaagccggca
    17461 tcgtgggctc caagatgggg gtgaacgccg ccggcatcgg attgtgtgtg aacggcctgg
    17521 ttacgccgcg cgacggcgcg aacggcatcc gcaagccctt ccatgtacgg tgcaaggaaa
    17581 tcctcgacgc gtggcgcttc gaccaggccc ttaccccggt cgtccagacc gaccggaccg
    17641 cgtcgagcaa ttttctcatc ggccatgccg acggcgagat catcaacatc gaggccactc
    17701 cggaccactg ctcctatatc tatccggttg acggtctcgt cgtgcacgcc aaccatctcg
    17761 tcaaggaagg ccgcgtcggc tcacagatgg agcgtatctc gtctaatacc ctttacaggg
    17821 ggccgcgcct cgagcgcttg ctgcgcgcca aactgggcag gatcgacttc gacgtcctgc
    17881 gcgaatgcat gtccgatcat ttttcggcac ccgctgcgat ctgccgccac gccgatacgg
    17941 ccttgccaga gcccaagcga gtgatcacgg tggccgcaat cgtgctcgat ctcaaggaac
    18001 gtgtcctcta cgcgaccgac ggtcagcctt gccagtcgga ttttcaggcg tttcccctca
    18061 gtaccgagac atccatcagc aacgcagcgt gaggaggctt caatgaacga tatgacacca
    18121 tttccgtggc cgaacggggc gcgctgcgca gtcatgctga cgttcgactt tgacgccgaa
    18181 acgctgtggc tgtcgcgcga tccagacaat gcgcgccgcc ccggcgtgct gtcgcagggc
    18241 gcgtatggcg ccagaaccgg tgtcccgaag atactggagc ttctcaagga agaggacctg
    18301 aaggccacgt tcttcgttcc ggggtggact gcagaaaagc atgtcgagcg cgtggaggcc
    18361 attgtcgcag gtggccacga ggtggggcat cacggctacc tgcacgaatg gatcgatccg
    18421 gattacccgg acaaggagcg cgaagctctg gaaaaggggc tcgaagcgct gaagaagacg
    18481 gtcggtgtga agcccgcggg ctaccgttcg cctgccggcg aaacgtccac gaacatgatc
    18541 agcctgctcc aggaatacgg cttcctctac gacagctcgc tcatggatca ggtcagccct
    18601 taccggcatg tgttgcagaa cggcgggcgc gggccggtcg aactgccttg gcactggagc
    18661 ctcgacgacg ctccctacgc gctgttcgcg atcaagtcgc cgcgcgccat catgaccaac
    18721 gagcacatcc tgagcatctg gaagcaggag ttcaccgaaa tctaccgctg gggcggtttg
    18781 gtcaacatcg tcacgcatcc gcaggtcatc ggacggccgt cgaggctggc gatgctgcgt
    18841 gagttcatag ctttcgtgcg gcgctcaccc ggcgtctggt tcgccaccgg acgcgaagtc
    18901 gccgaggcgt gggcggcaaa ggaaacggcg tgatcgagag tcggcagcgg tgatactgtt
    18961 tttgcaaacc acgctgaacg gtctggtgct gggaggtatc tattctctgg ccgctgtcgg
    19021 cttttccctg atcttcggtg tcacgggtat cgtcaatctc acccacggga tctttgttgt
    19081 cgcgggagcc tacggagcca tgctgctcta tcgcgaagtc gggcttgatc ctttgctcgc
    19141 tgtgatcccg attggggcgg tgcttttcgc cgtcggatat atctaccaga gaacgattat
    19201 ccactgggcg atctcgcggg cctcgctggt cgcgtcgctt gtcgtcacct tcggcgtggc
    19261 catggtcgcc cgcaacctgc ttcagcttgg cttcgggcct gacgttcaga cgatcacgcc
    19321 aagctacagc ttcacgagcc tggctctcgg gccactgagg atcgacctcg tacgcctcct
    19381 tgccctgggt gccagcctcg ttctgctgac cgctctggcg ctggtgctgt cgcgcatgca
    19441 tttcggacgg gccgtacggg cgacggcgca gcagccgctt gccgccgcct tgtcggggct
    19501 caacgtgaac aacctgcacg gtctcacctt cggcgtcagc gccgcgatgg ccggcgcctc
    19561 cggggcgatc atcggcatcg tccagccgtt ctcggcggca agtgaggtcg cctggacgct
    19621 gaacgccttc atcgtcgtcg ttctcggcgg catcggcagc cctgcgggcg ctctgctggg
    19681 cggcctgctg cttggcctca tcaacgctta cacggcccaa tatgtcggtc cggcgctgac
    19741 cacggcaatg atgtttctcg ttctcgtcat catgctggtc gtgcgtcccc aaggcctgct
    19801 tggaaacagc tttggggaat ccagatgaac cgctttctgg ctgcgtttgc agggctggct
    19861 gccgttgcgg tcatcgcact gctgccgctg tggctcggtc cattctacac ccgggttatt
    19921 cagttcttct tcttttcggc agcgcttgcg attgcgtgga atattctggg cggatttgcc
    19981 ggctactgga gcttcggcca cactgctttc atcggcattg gcgcctttgc cgcagcccat
    20041 ttcgtaatgc agttcggcgc ttttggcact ggtcccgtct cgatggtatt gccggtactg
    20101 gtggcggcgc tggtctgtgc cgtcttcgcc gccctgcttg cctatccggt gctcaggctt
    20161 cgcggcatct atttcgcaat cgtgatgctg ggcgttgccc aggttgtcag cgagcttgtc
    20221 aacaacgtca actggtttca gggcggcgtc ggcgtgtacc tgccctcgcc ggttccgagt
    20281 ggcatggctc ccgaggactt ctactactac gtattcgctg cgctgttgct ggtgacgttc
    20341 cttctcagcg tcgccatacg acgcacgcgc ttcgcctatg gcctcctggc gatccgcgag
    20401 gacgaggaca cggccatgat gctgggggtt ccgaccgagc ggttcaagat agccgctttc
    20461 gtgctttcat ctgttctggt gggagttctc ggcgccgttt atgcctacag cgtcggctac
    20521 tttacgacct acacggtttt ccggctcgac ttcagcttga acatgatcgt cttctgcctc
    20581 atcggcggca taggcacgct gttcggcccg atcgttggaa ccgcggtaat gttgttcctg
    20641 acgcaggtgc tgctgtcgcg cttcctcgac atccaccttc tgatcaccgg cctgattgtc
    20701 gtgaccatca ccctggtggc gccaggcggc atccttggca tcctgcggcg gcgcccccgc
    20761 cgcgccaagc tggcggctga gccggaagcg gagcgaagcg catgagcgag atcatccttt
    20821 ccgggcgggg tgtcacgaag cgtttccgcg ggcttaccgc cattgaatct gtcgactttg
    20881 agatacctgt cggcgcaatc tacggcctga tcggcccgaa cggtgccggt aagtcaacgc
    20941 tgttcaacct gatcaccggc tactatgacc cgagcgcggg cgaaatcagc ttccgcggca
    21001 agtcgctggc aggggtgccg accttcaggc gaaaccagct cgggattgcg cgggcattcc
    21061 agatttcaaa gccgtttccc gctctgacgg tgcgcgagaa tgtcgccgtc ggcgcaatgt
    21121 tcggcagccc tgaccggacg gatgtggagc atgtgaccac cgaggcgttg cgcatcgccg
    21181 gcatcgagga tcttgccgac cgcaccgcag aaggtctcac tgtcggggcg ctgcgcaagc
    21241 tggagatcgc ccgtgccatc gccacgcggc cgaaactgct gctggccgac gagccctgcg
    21301 ccggccttaa tcccaccgag acggccgaga tggtttcctg cctgcgcaat atccgcgggc
    21361 acggtgtcac cgtgtggctg gtcgagcacg acatgaaggc agtgaccagc atctgcgaca
    21421 ggatcattgt catcgatgcc ggccgcaaga ttgccgaggg aacaccccag caggtggtca
    21481 gtgatccgaa ggtcatcgcc gcctatctcg gcacgccggt cgccgaggag accgcccccg
    21541 gaccgggcta ggcggcatct tgctccagga accagaatga cggaattgcg cgggatcagc
    21601 gcgtcaggca acggcgcgaa tcgcatcggc gttgagatcg gcggcacctt caccgatctg
    21661 gtttgggtcg cacccgacgg acagttgcgc accggcaaaa cgccgtcgac gccgcaggcg
    21721 attcatgagg ccgtgctgaa ggcgatagac gaagtcggtg cgccgctcgc ctcggtggag
    21781 caggtcaccc acggctcgac ggtcgccacc aatgcgcttc tgacgcgacg cggcgcgcat
    21841 gctggccttg tcaccaccgc cggctttcgc gatgtgctca tcgtggggcg gggcgatcgc
    21901 gataacaaca tctacgacat gcagtatcgg cgccctgcac cgccgatccg ccgcagcatg
    21961 atccgcgagg ttcccgagcg cattggcccg cacggcgagg tgctcctgcc gctcgacgaa
    22021 gaggccgcct ggcgggagat ttcggaattg atcgatcagg gcgtcgaggc aatcgcgatc
    22081 tgcctgctgc attcctattg caatccggcg catgagcgtc gacttgccga gatgatccgg
    22141 gagcgcgcgc cgcatgtcgc ggtggccgcg agccacgagg tgtcgccgga attccgcgag
    22201 tacgagcgca gcatgaccac ggtggtcaac gcctttgtcg gtccggtggt cgaacgctac
    22261 gtcgaccgcc tggatgcggc gctgcgtgat cggggctatg gtggcgtgtt gcagatcatg
    22321 cagtccaacg ggggcagcat gccggcggtt tccgccggcg cgaacgccgt gcgcatgctg
    22381 ctttcggggc ccgccgccgg ggtccgcgct gccatgtggt ttgccgcccg caatggcatc
    22441 cagaatgcga tcacgctcga tatgggcggc accagcaccg atgtcgccat cgcgccggga
    22501 ttgacacccg gcatcgttcc ggagcttcga gtggatggct tgccagtgcg gaccgcctcg
    22561 atcgacatgg ccacgatcgg cgccggaggt ggcagcatcg cctccatcga caggggcggc
    22621 ttcctcgccg tcggtcccga aagcgcgggc gcgttgcccg gaccggcttg ctacgggcgc
    22681 ggcggcgaat gggcgacggt gactgacgcc caggtcgtgg ccgggctcct gcgacccgac
    22741 cacttcctgg gcggccggat cacactgtca accgataagg ccagagccgc tctcgacgcg
    22801 ctcggcatgt ctggaggagc ggaggcggcg gcggatgcgg tcctgcggct cgtcaacagc
    22861 agcatggcgg atgcggtgcg gttggtctcc acacggcgtg gtatagaccc ccgcgacttc
    22921 gccatcgtcg cctatggtgg cggcgggccg cttcatgcag ccatggtggc cgaggaactc
    22981 ggcgtgcgtc aggttctggt tccgtggtcg ccgggcctcg ccagcgcttt cggtctgctg
    23041 attgccgaca cggtgatcga cgtggcccgc agcagcctgc atcgcctgtc cggcgagact
    23101 ctggacgaaa ctcgcctttc cgaactcaag gagtccgcga cggaggcggc gcgccggaac
    23161 gggctgacac agggaagcta cgaagtcgca atcgggctcg acatgcgtta cgccgggcag
    23221 gctttcgaac tgactgtctg gccgggcacc ggcgccgccg cagccgcgga attgcggggg
    23281 ctcttcgaag ccgagcacga gcgccgctac ggttatgcgc gcgccacgct cgcagtggaa
    23341 gtcgtgggat accggatccg cgtcatccgc aggtcgggcg gggcgatgtc cacccctgtc
    23401 cccgctggcg gagggccggc ccccatctcc ctgcctgcca ccatcggtgg gcagaggatg
    23461 aatgtctcgt tcatggcgcg cgactcgctt ttccccggcg cctgcatcga tggcccggcg
    23521 gtcgtggagg agcccacgtc gacgactgtc gtaccgcccg gatggcggct ccaatgcctg
    23581 gtgacgggcg acctgctctt gagggatgcg cgatgactcg ggacaagacc cggctgatca
    23641 tcttggcacg cggcctgcag gcggctgccg acgagatggc cgccaatctg attcgctcgg
    23701 ccttttctgc cgtggtgcgc gaggcgcgcg actgttcgac cgcgcttctc gacgcccagg
    23761 ggcgcgtcgt cgcacaggca gacatgatcc cgatgcagac tgcggccctg agcggctcct
    23821 ttcaggccgc tgcggagcag ctcgatctgt ccggcgtccg cccggggcag ttcgtgctca
    23881 tgaacgatcc ctacagcggc gggcagcatc tcaacgacat cattctgttc agcccgatct
    23941 tcttcgaaga tgccctgatc ggctggagcg gcagcaccgc gcaccatctc gacatcggcg
    24001 gcggttccgc gggcgtcaac acctcggcaa ccgacctgat ccaggaaggc atcgtcattc
    24061 cgccgctgct tcttgatatc gagcgcgact ggcatggcgg tatggtcgag cgactgatct
    24121 tcgccaatat ccgcacgccg gaaatcggcc tcggcgacat ggatgcccag tttgccgcca
    24181 atcacgttgg tgccgagcgc gtcatggaga tggcaaggcg gttcgggccc gaagtggtgg
    24241 cagacgccat gcgcgaggtc cttgactaca gcgagcggcg aatgcgcgcg gcgatcgagg
    24301 agattcccga cggtagctgg cgcggcgagg cgttcctgga cggcgacggc cgtgatccgc
    24361 aaggcccgcc gatccgcatc gtcgccactg taaccatcaa gggtgacgag gcgatcctcg
    24421 atttcaccgg caccgatccc caggtgcgta ccatgttcaa ttcgccgctc gccagcagca
    24481 tcgccggcgg cgtgacggcc ctgcgcagca tcctcggcga tcgcgacatg cccgccaacg
    24541 acggatgcaa ccggccgctg accctcatca tcccggaggg cacattgctc aacccgcgcc
    24601 cgggcgcgcc ggtaagagcc cgcgccaccg cctgctgccg tgcgctcgac gcggtgcacg
    24661 ccgcgctcgg gcaggtcctg ccggagcgga cgccggcgca gggcgccaat tcgacgaccg
    24721 gcttcttcct cacccatgcc cgtcctgatg gcggcgtcga catccatctc gatgtgctgg
    24781 gtgggggatg gggagccgcc aagggctatg acgccatcca tgctaccgac catgttctgt
    24841 cctcctgccg gttgacgccg acggagtcga tcgaacagct aagcccgcat ctgctcatcg
    24901 aaagtttcgg cctcatccag gattcttggg gcgctggcgc cttcaacggc ggtatggggc
    24961 tgttccgccg gttccgcgtt ctgaaggagg atgtcaccct cagcctctac tcggaccgtt
    25021 tccgtctgcc cccgaaaggg cgcgagggcg gatgtgacgg aacgtgcgcc agcctttttg
    25081 tgctgcgcga cggcgagagg atcgaactcg gcgctaccag caccttcgcg ttaaagcgtg
    25141 gcgatctggt cgagatccgc ctgccgggcg gcggtggctg gggaaatccg cgggagcgcg
    25201 atcgtgaact ggtcgttcgc gatctggagg acgggctgat ctcccgcgat ttcgcccgcg
    25261 acagatacgg gctggatgtc gaccggtggg ctccggtcga ggatcggtcc gcagacacga
    25321 aattaacgac aggttgagca tgcgtaacca ggctagcggt catgaactcc tgcatcccgc
    25381 cgatatcgac gttgctccgc tcatgcaggg cattatcgac tgggtggaaa tggagacgcc
    25441 ctccgacaga ccggatctga tcgaccggct gctggaccat gtcgaacgct ctttcgacaa
    25501 tctgccggtg acggtgcgcc gcattcccgg ccgcgacggc tgcggcgggc agctcgtgct
    25561 cacctatgcg ccggcgggat gcgaaggcaa accggcactc atcatgggtc atgtcgatac
    25621 ggtgtgggct gccggcacgc tttccgagcg ccccgtgcgc cgcgaggacg atcgccttta
    25681 cggtccgggc atcttcgaca tgaaggcggg ctcttacctc gccaccgaga ccatgaggcg
    25741 catcgctgcg gccggcctcg ttccgccgcg gccgatcacg gtcttcctga acagtgacga
    25801 ggagatcggc agcccgacat cgcgcgacac gatcgaggag ctggcacaaa aagccgcctt
    25861 cgtgctggtt cccgaaccgg ccttcgaggc accgggaacc gtgatcacgg cacgaaaggg
    25921 ctgggcgcga ttctccatca ccgctcacgg ccgctcctcg catgccggtg gcaatctcgc
    25981 agacgggcgc agcgccatcc gcgagatcgc cagacacatc cttgacatcg agggactgaa
    26041 cgacacggaa gagaaagcca ccttcaatgt tggcacggta aaaggcggaa cgctcctcaa
    26101 cgtggtgccg gccgaggcaa ggatcgagat cgacatgcgc gcagaggaca tggccaccgc
    26161 cgagcggctg gtgaacgaaa tgctggcgcg cagacccttc gatcccgaca tcaggatcga
    26221 agtcgagggg ggcctgaatc gtccgacttt cgagcggagc gccaaagtgg cccggctcta
    26281 cgaggcgacc tgcgcgctcg ccgaagagct tggcctgcct atggccgaga cttcgcgcgg
    26341 cggcgtgtcc gacggcaatt tcgcggccgc attgggactg ccggttctcg atggtctcgg
    26401 ctgcaacggg gcgggtgcgc atgcggtcga tgagcacatt ctcatatcga cgatcgcgcc
    26461 gcgtcacgcg ctgatccatg gcatgttcat gtcgcgccgc tttcaggaaa tggcgctgga
    26521 ggaagcctga tccggtgccg ctgcctttgc attttcactt ggggaggatg attgggtgac
    26581 ggcttcgatc agcagcccct gcgccgtggg gagctgccct catcatgcgg caggccggcc
    26641 tgctcggggc aaagccaaga aggctgcgga caggtcctga cattcttctg aggctgattt
    26701 aagtgggttt acggtggccg gcagatccga tccggccgtg acgtctatca atgcgatcgg
    26761 gatacaggcc gactggcaga cgttcccgat accgtcgctg agagggagct gaaaagtgcc
    26821 ggggttgttc tccaagtttg aatgcggacc gctcgagttg cccaaccgga ttgtcgtctc
    26881 gccgatgtgc cagtactccg cggagaatgg ctgcgcgggc gactggcatc ttcagcatct
    26941 gatgacgctg gcgatgtcgg gagccggact ggtgagcttc gaagcaaccg cagtggagcc
    27001 cgccggccgg atcacccatg gcgacctggg cctcttttcc gatgaaaacg aacgttcgat
    27061 caaacgcgtg ttggaagccg cgcgctcggt ggcgccagcc ggaaccaggt tcgccatcca
    27121 gctcggtcat gcaggccgca aggcgtcaac ccagcggccc tgggaaggcg gcggcccgct
    27181 tgcggcggag gaaaatccct ggcagacggt cgctccatcc gccatcccct tcgccgaagg
    27241 ctggcacgtg cctgaggcgc tggacgaggc cggtctgagg cgcatccgcg atgcgttcgt
    27301 cgaatctgcc aggcgggcgg tgcggctcgg cttcgatgtg atcgacctgc atcttgcaca
    27361 cggatacctc ctgcaccagt tccattcccc gctcgccaac cagcgcaccg atgcctgggg
    27421 cggcgatggc gaacgccgac tcgcttttcc cttgtccgtg gcgcgcgcag tacgagaagc
    27481 tgtccctgaa tcggtccttg tttccgcacg cattacggga agcgactggc gcgaggacgg
    27541 gctgacgccg gaagatgccg tcgccatggc aacggctctg aaggccgcgg ggatcggcat
    27601 ggtttgcgtg acttcgggtg gtgtcgcccc cgcgccgatc aaggctggcc ccggctacca
    27661 ggtgccattt gcagagaagg tgaagcggga atcgggtctg cccacccagg ccgttggaat
    27721 gatcatgaca gccgaacatg ccgattcggt catagccgag ggcaaggccg atcttgtggc
    27781 cctggcacgt cctctgctcg atgatccgcg ctggccttgg caggcggcac gcaaattggg
    27841 cgcgcaggtc gacgtacctc cgcagtaccg gcgtgcggtg ccgccggcat ggccgggtcg
    27901 acccgcatga gcgccgggaa gaaaccgcag cccggtatcg tctgcgagac ttcggcggct
    27961 caaccttcgc ctatgagctc cgcaaagaag tggccagcca agacgaacag attcatcggg
    28021 tttccccacc tgctttgaga accggaaacg gtcaattctg cagtttcaat tccggacagt
    28081 aggtagggag cactgcaagt gcatgacctg taacaccacg tttgccttcg gcgagggtgt
    28141 gtcaggggcg gcggcgaata cggccggtgg cggactaggc tggttcatcc tgccacacgt
    28201 gccaaagata tgcagacaaa cgtttggcgg cagcatacta gggtctggat gctaggcgcc
    28261 gtttttatcg atacgccgct gttcctggat tgaggatcgg ccggcacgac gacgctgtcg
    28321 gtgctcatct tccagttctg ggcggacgcg cggcgagtcg gcggcgctga ccgtcgttct
    28381 gttgctgctg actttggcaa tcttcctgcc gctgtcgggt gccttctgcc gtgcctgccg
    28441 aaagataccc tgtccgttga atagtccgca cctcggagat ttcgctgctg gggtacgact
    28501 tcgcctccaa cccggacagc agcgtcctga atttggtcat gtaggagtgc cgcaacgaac
    28561 cccgtctctg gaatgaccgc caccggttga tcgtctcgtg tacgtcgacg ggaaacgcct
    28621 gaccggcgac caaggcctta agcgctcggc gcaacccgac caaaccttga catcggatat
    28681 atatccgaaa tgaatgaagc agacgtggag gatatgaatg gcggagatat cgctctcacg
    28741 ggtgtcgaag agctacggaa gcgtcgaagt gatccgcgac gtgagcctcg acatcggatc
    28801 gggcgaactg g
//
LOCUS       DASVDU010000026        10750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_727914, whole genome shotgun sequence.
ACCESSION   DASVDU010000026 DASVDU010000000
VERSION     DASVDU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10750
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727914"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..817
                     /locus_tag="VF226_02040"
     CDS             374..817
                     /locus_tag="VF226_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072795.1"
                     /translation="MRAYLVVAAFCSALAGLAVFLPGGAGAGEGQTGPLRCTFEAGTS
                     IAYAGGSYEPSPAKALAFNIADIDLERQEAVLQTGSGGQGHLKIVRAVNANHFLEVVN
                     EGFLNITTVYDIDPQRKAHPAVHSRHLGLLGEPVVAQYYGFCTPG"
     gene            850..2805
                     /locus_tag="VF226_02045"
     CDS             850..2805
                     /locus_tag="VF226_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7072796.1"
                     /translation="MLKRILIANRGEIALRIIRTARRLGIETVAVASEPDLRMPFARA
                     ADVLVPIGPGPASESYLAQDRIIAAAKATGAEAIHPGYGFLAENAAFAESVRSEGLIF
                     IGPTADAMRKLGGKAEAKAIAIKAGVPVVPGYQGEDQRPEILAEAARGIGFPVMIKAV
                     AGGGGRGMRLVEQEADFAQLLASAQREAQAAFGDPRILLEKVVQAPRHIEVQVFGDSH
                     GNVVHLFERDCSLQRRNQKVIEEAPAPGMSPQLRKKMTAAAVKLAKAVGYQGAGTIEF
                     LVEGGGLGANAPWYFIEMNTRLQVEHPVTEEITGLDLVEWQLRVASGEPLPLEQDQIT
                     FSGHAIEARLCAEDAARGFLPAIGRIVAFETPAVDGLRVDAGVEAGSEVSSYYDSMIA
                     KLIGSGPDRATALARLIEALEQTTVAGVKTNAAFLHELLVHPAFRNGEMDTGFIGRDL
                     ASLSSNTSDPTAIAQGAAALIRARQQALELERQRFSPERHSPWAKLDGFQLGPARRYP
                     LDVVVEGAKTTVEASWGEDGLSIAMPGQAAVAPQGRLATVADGDRVFVLHDLRHTEVS
                     WPVYDALAAEEHSNGGTLRAPINGRIVKVFVEEGATVDKGQRIVVIEAMKMEHVLVAP
                     RAGTVTKLTATEGQQVTEGAVLASVDE"
     gene            2817..3818
                     /locus_tag="VF226_02050"
     CDS             2817..3818
                     /locus_tag="VF226_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX7072797.1"
                     /translation="MPARLNLITDIRGLRVGNAHDERVLSGVTVVIFDKPNVASGVTH
                     GGAPGSRDTTLLEPEMTIQSLDAVVLSGGSMFGLDAAGGVVSFLRQQGRGVRIGSANV
                     PIASQAIVFDLLNGGDKNWGRMPPYWDLGWQAAESAAAGRFPLGTVGGGYGASTVNFK
                     GGLGSASVTTRSGFEVGALVVVNAVGSTTIGDGPHFWAGAYEIGREFGGHGLPPRVPP
                     KAREFRIKGAAPPSTTIALVATNAQLNKAETKRLAIMADDGFSRAIRPSHAPMDGDTV
                     IAVSTQDEPLENRSVELAEIGAAAADCVARAIARGVYEAAVPSESWAGPPAYRDLYP"
     gene            3815..4006
                     /locus_tag="VF226_02055"
     CDS             3815..4006
                     /locus_tag="VF226_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072798.1"
                     /translation="MTRARWAVVIAVSVILLGLLAWQQTRQREIRRCLEAGGIWDGPN
                     SRCVPDSARPILQRDLHRT"
     gene            complement(4009..4641)
                     /locus_tag="VF226_02060"
     CDS             complement(4009..4641)
                     /locus_tag="VF226_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX7072799.1"
                     /translation="MPLARIAIVYHSGWGHTARQAEAVRAGVEDVEGTEALLMSVEEA
                     QNRWDDLASTDAIIFGSPTYMGGVSAPFKAFQEATSKAVMSKGFAWKNKIAAGFTNSG
                     ARSGDKLATLVQISLFAAQHGMHWINLDLPPANNSTTGSEQDLNRLGFWLGAAAQSNT
                     DQGADVAPPQSDLSTARHLGRRVAEVTLQFIRGRIDEERAVGYGRIAASS"
     gene            complement(4655..5101)
                     /locus_tag="VF226_02065"
     CDS             complement(4655..5101)
                     /locus_tag="VF226_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7072800.1"
                     /translation="MSETGTTRSLVFERQMKHPPEKVWRALTQSHLIAEWLMENDFVP
                     KVGHRFTFRATPLPGWSGIANCEVLAVDPPHLLSYRWGDGTESASGLVTVVTWTLMPR
                     DGGTLVRMEQSGFRTPDELAFKRMGKGWPRVLERLEDVVGRVDLDA"
     gene            complement(5116..5439)
                     /locus_tag="VF226_02070"
     CDS             complement(5116..5439)
                     /locus_tag="VF226_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7072801.1"
                     /translation="MHVPPDHLFAALADPTRRGIFERLSRGGEQSVRALTAEAGVSQP
                     AVSKHLGVLKKAGLVRDRRAGRETHYSANPKALAPLVAWLSFYAAFWSARLDKLEELL
                     ERMDQ"
     gene            complement(5524..6768)
                     /locus_tag="VF226_02075"
     CDS             complement(5524..6768)
                     /locus_tag="VF226_02075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7072802.1"
                     /translation="MTSDVLVLGAGMVGVSAALHLQKRGRSVVLVDRRGAAEETSYGN
                     AGLIQREGIVPYGFPRDIKKLWRYALNMLPEAHHHWSALPSNAVWLFKYWRYSTPARL
                     AASARANRPLVERCIIEHRALMEDAGVTDMIRETGYLKIFRTHERLAEYTREQEEIRR
                     EFGINFEELDPAAIRKREPYLTGNVVGGLLMTDPVSIADPGALGKAYADLFVKRGGKF
                     ITADARTLEATMDGWQVHTVDGPVKAREAVIALGPWSDDVLKPLGIRVPLGVKRGYHV
                     HFRPRGDAVLNGPMFDIENGFVLAPMTRGIRLTTGAEFAPRDAPRTPVQLDRLEPVAR
                     ELFPLADRVEPKPWMGARPCLPDMLPMIGPVPGHRGLWADFGHHHLGFTLGPATGRLL
                     AEMMTGEPTYTDPWPYRVDRFR"
     gene            6883..8190
                     /gene="purB"
                     /locus_tag="VF226_02080"
     CDS             6883..8190
                     /gene="purB"
                     /locus_tag="VF226_02080"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7072803.1"
                     /translation="MIPRYSRPEMVRIWAPETRFRIWFEIEAHAATAMADLGIIPKEA
                     AQAVWEKGSAAEFDVARIDEIERVTKHDVIAFLTHLAEHVGPEARFVHQGMTSSDVLD
                     TCLNVQLVRAADILLADLDRVLAALKKRAFEHKNTVTIGRSHGIHAEPTTFGVKLAYA
                     FAEFSRARQRLEHARSEVSTCAISGAVGTFANVDPRVEEYVAKQLGLTPEPVSTQVIP
                     RDRHAMYFATLAVIASSIERLAIEIRHLQRTEVLEAEEYFSEGQKGSSAMPHKRNPVL
                     TENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPDATVTLDFALNRLAGVI
                     ENLVVYPENMQKNLDRLGGLIHSQRVLLALTQAGVSREDAYRLVQRNAMPVWRGEGDF
                     LSLLAADPEVSAKLSRAQLESLFDLGYHTKHVDTIFRRVFGES"
     gene            8198..8776
                     /locus_tag="VF226_02085"
     CDS             8198..8776
                     /locus_tag="VF226_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7072804.1"
                     /translation="MRTSEVDILLVPDWTGAGPDHWQSRWERSLSTARIISQGDFDTP
                     RKDDWVGRIVEAVRKARRPVVLVAHSCGVAAVAHAAPFLAGLDVAGAYLVAPADLEQT
                     EDWPVARGDFTPMPLDRLPFPAVLVASSSDPYCSMERARTFADAWGATLVPAGDAGHI
                     DAASGHGPWPEGLMRFGLFLSRLGPAKRKLDS"
     gene            complement(9232..9303)
                     /locus_tag="VF226_02090"
     tRNA            complement(9232..9303)
                     /locus_tag="VF226_02090"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9269..9271),aa:Val,seq:cac)
     gene            9662..9892
                     /locus_tag="VF226_02095"
     CDS             9662..9892
                     /locus_tag="VF226_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072805.1"
                     /translation="MINHTVLAGAFAAALLSLAAADAAVAGSKAQAINPECPNKYAVC
                     PPKLKAKEKPDSVPMKAVPASKTVKPKKERSR"
     gene            complement(9981..10055)
                     /locus_tag="VF226_02100"
     tRNA            complement(9981..10055)
                     /locus_tag="VF226_02100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10021..10023),aa:Val,seq:cac)
     gene            complement(10105..>10750)
                     /locus_tag="VF226_02105"
     CDS             complement(10105..>10750)
                     /locus_tag="VF226_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072806.1"
                     /translation="VYVTGEQQTASGPYMKYILLFREPSATALVSINVPRSSFENGSA
                     EPRTFEQILASARIDPKAGEKPFELGYMGPFRDTGAFIGQSYFFAIEDRPAGGNGAAA
                     SQQPSLVIAASLDGPHIDDLDATGRAGIAALAGGREPQGLESRPLEVAGLEGIEQVVP
                     PSGTGLDTGIYQVILRRKDGGYYRIVGRAPAAEWPALLPEFRKIAQGFTPRLKA"
BASE COUNT         1890 a         3359 c         3621 g         1880 t
ORIGIN      
        1 cagaatgaac ccgctggccg cgaaaatggc aatccgcgct attgcaaaac gccgcatgaa
       61 aatcagcccc caagcgaaac tgcggaactc gggtccttcc gtgcccgaca tattatgctt
      121 acttttttgc taatatccag attcatttgc accggcaatg attggcgcac ggagaatcgg
      181 gggtgttggt taattcagac aggaaccgtg gcttaaatgc cacaatctcg aatgtgaggc
      241 ggggcatatg gcgggtgttg agcgtcggtg ccggcgctgg cgcgttactg agcgccacaa
      301 tcagcctatt gcacccgggc ccccgcgcgg gttgaatgcg gcgcactgga aatcggggaa
      361 ggcctcacta ctcatgagag cttatctagt cgtagctgct ttctgcagcg ccctcgcagg
      421 cttggctgtg ttcctgccgg gaggcgccgg agccggcgag gggcagacag ggccgctcag
      481 atgcaccttc gaggccggca ccagcatcgc ttacgccggc ggctcctatg agccttcgcc
      541 ggcgaaggcg ctggcgttca acatcgccga catcgacctc gagcgccagg aggccgtgct
      601 gcagacgggc agtggtggcc agggccacct caagatcgtg cgggccgtca acgccaatca
      661 cttcctcgag gtcgtcaacg agggcttcct caatataacg accgtctacg acatcgatcc
      721 gcagcgcaag gcacatcccg cggtacactc ccgccatctg ggcctgctcg gcgagcctgt
      781 cgttgcgcag tactatggat tctgcacacc gggctgagag gagaccagga ggggatttgg
      841 aagccggcca tgctgaagcg gattctgatc gccaaccggg gtgaaatcgc gctgcgcatc
      901 atccgcacgg cgcgccggct cggaattgag acggtcgccg tcgcgtccga gcccgacttg
      961 cggatgccct tcgcgcgcgc ggccgacgtc ctggtgccga tcgggccggg gccggcgagt
     1021 gagagttatc tcgcccagga caggatcatt gcggcggcca aggcgacggg cgcggaggcc
     1081 attcaccccg gatatggctt tctcgcggag aacgccgcgt ttgccgaatc ggttaggtcc
     1141 gagggcctga tcttcatcgg gccgactgcc gacgcgatgc gtaagctggg cggcaaggcg
     1201 gaggcgaagg cgatcgccat caaggccggc gtgccggtcg tcccgggcta ccagggcgag
     1261 gaccagcgcc ccgaaatcct cgccgaagcc gcacgcggga tcggctttcc ggtgatgatc
     1321 aaagctgttg ccggcggtgg cggccggggt atgcggctgg tggagcagga agccgacttt
     1381 gcccagctcc tcgcgagcgc tcagcgcgag gcccaggctg cgttcgggga tccgcggatt
     1441 ctgctcgaga aggtggtcca ggcgccgcgc catatcgaag ttcaggtctt tggcgacagc
     1501 catggcaacg tggttcacct gttcgagcgc gactgctcgc tgcagcgacg caatcagaag
     1561 gtgatcgagg aagcacccgc tcccggcatg tcgccgcagc tccggaagaa gatgacagcc
     1621 gcagccgtca agctggccaa ggcggtcggc tatcagggcg ccggcacgat cgagttcctg
     1681 gtggagggcg gtggccttgg cgccaacgcg ccgtggtact tcatcgagat gaacacgcgg
     1741 cttcaggtcg agcatccggt cacggaagag attaccgggc tcgatctggt cgagtggcag
     1801 cttcgtgtgg cctcgggaga gccgttgccg ctggagcagg atcagatcac gttttccggg
     1861 cacgcgatcg aggcgcggct ttgcgccgaa gatgcggcgc gcggcttcct gcccgcgatc
     1921 gggcgcatcg tcgccttcga aacaccagcg gtagacggcc tgcgggttga tgccggcgtc
     1981 gaggccgggt cggaagtctc ttcttattat gattcgatga tcgccaagct gatcggctcc
     2041 ggccccgacc gcgcgaccgc gcttgcaaga ctgatcgaag ccttggaaca aacgacagtc
     2101 gccggcgtga agacaaacgc cgccttcctg cacgagctgc tggtgcatcc ggcttttcgt
     2161 aacggcgaga tggatacggg gttcatcggg cgagacctcg ccagcctgtc gtcaaatacg
     2221 agcgacccga cagctattgc gcaaggggct gcggcgttga tccgggccag gcagcaggcg
     2281 ctggaattgg agcggcagcg gttctcgccc gaacggcatt cgccttgggc gaagctcgac
     2341 ggctttcagc tcgggcccgc gcgccggtat ccactcgacg ttgtcgtcga aggcgcgaaa
     2401 acgacggtcg aggccagttg gggagaggac ggcctgtcca ttgcgatgcc cggccaggct
     2461 gccgttgcac cgcagggccg tcttgctacc gtcgcggatg gcgatcgcgt cttcgtgctg
     2521 cacgatttgc ggcatacgga ggtgagctgg ccggtctatg acgcgctcgc agcggaagag
     2581 cacagcaacg gcgggaccct tcgcgccccc atcaacggcc ggatcgtcaa ggtttttgtc
     2641 gaggaaggcg cgaccgtcga caaggggcaa cgcatcgtgg tcatcgaggc gatgaaaatg
     2701 gagcatgtgc tcgtggcgcc gcgggccgga acggtcacga agttgacggc aacggagggc
     2761 cagcaggtga cggaaggcgc ggtgctggct tcggtcgacg agtagaggga gaggtcgtgc
     2821 cggcgcgcct caatctcatc accgatatcc gcggcctcag ggtcggcaat gcgcatgatg
     2881 agcgcgtgct gagcggcgtt acggtcgtca tcttcgacaa gccgaatgta gcctcgggcg
     2941 ttacacacgg cggcgccccg ggcagccgcg atacgacgct gctcgagccg gagatgacga
     3001 tccagagcct ggacgcggtg gtgctttctg gcggatcgat gttcgggctc gatgcggccg
     3061 gaggcgtggt ttcctttctg cgccagcaag gccgaggcgt tcgcatcggg tcggcgaacg
     3121 tcccgatcgc ctcgcaggcc atcgtgttcg atctcctgaa cggtggcgac aagaactggg
     3181 gtcgcatgcc gccgtactgg gacctcggct ggcaggcggc ggagtcagct gctgctggcc
     3241 gatttccgct cggcaccgtc ggcggcggat acggcgccag cacggtgaat ttcaaaggcg
     3301 gcctgggctc tgccagcgtc accacgcgat ccggcttcga ggtcggcgcc ctcgtggtcg
     3361 tgaacgctgt cggctccacg accatcggcg atggcccgca cttctgggct ggcgcctacg
     3421 agatcggacg cgagttcgga ggacatgggc tgcccccgcg ggtgccgccg aaagcgcgtg
     3481 agttcaggat caagggggca gcacctcctt cgacgacgat cgctctcgtc gctaccaatg
     3541 cgcagctcaa caaggcagag acgaagcggc tggccatcat ggccgatgat ggtttcagcc
     3601 gcgccatccg cccttcgcac gcaccgatgg acggcgacac ggtaatcgcg gtttccaccc
     3661 aggacgagcc gctggagaat cgtagcgtcg aactcgccga gatcggtgcg gccgcagccg
     3721 actgcgtggc acgcgccatc gcccgcggag tctatgaggc cgccgtgcca agcgagagct
     3781 gggccggtcc gccggcgtat cgggatctct acccgtgacg cgggcgcgct gggctgtcgt
     3841 gattgccgtg tccgtcatcc tgctcgggct gctggcgtgg cagcagacga ggcaacgcga
     3901 gatcaggcgc tgcctcgagg ctggcggtat ctgggatggt ccgaactcgc gctgcgttcc
     3961 tgattcggcg cgccctatcc tgcagcgaga ccttcatcgc acgtagcatc agctcgatgc
     4021 ggcgatccgc ccatagccta cagccctttc ttcatcgatc cgaccccgga tgaactgcag
     4081 cgtcacctcg gcgacgcgtc gcccgaggtg acgcgccgtc gagagatccg attggggtgg
     4141 tgcgacgtca gcgccctggt ccgtgttgga ttgagctgcg gcgcccagcc agaagccgag
     4201 ccgattgagg tcctgctccg agcctgttgt ggagttgttg gcaggcggca gatcgagatt
     4261 gatccaatgc atgccgtgct gggcagcgaa aagcgagatc tgaaccagcg tcgccagctt
     4321 gtcgccggag cgggcgcccg agttggtgaa accggcagcg atcttgttct tccatgcgaa
     4381 ccccttgctc atgaccgcct tagaggtcgc ttcctggaag gctttgaacg gtgcggagac
     4441 gccgcccatg taggttggtg acccgaagat gatggcatcc gtcgaggcca gatcatccca
     4501 gcggttctgc gcttcttcaa cactcatcaa gagcgcctcg gtgccttcga cgtcctcgac
     4561 gccggccctc acggcctcgg cctgtcgggc cgtgtgaccc cagccgctgt gatacacgat
     4621 agcgatgcgg gcgagcggca tggcaacctc cgaattaagc atccaaatct acgcggccaa
     4681 cgacgtcctc gaggcgctcg aggacgcgtg gccagccctt acccatgcgc ttgaaagcca
     4741 gctcgtccgg cgtcctgaag ccggactgct ccatccgaac gagcgtgccg ccgtctcgcg
     4801 gcatcagcgt ccaggtcacg acggtgacga ggccgctggc cgattccgtg ccgtctcccc
     4861 agcggtagct gagcagatgc ggcggatcga cggccagcac ctcgcaattg gcaatgccgg
     4921 accagccggg caggggagta gcgcggaagg tgaagcgatg gccgaccttg ggaacgaaat
     4981 cgttctccat gagccattcg gcgatcaggt ggctttgtgt cagtgcccgc cacacctttt
     5041 cgggtggatg cttcatctgc cgctcgaaga cgagtgagcg ggtggtgccg gtctcgctca
     5101 tcggtatttc tccggctact gatccatgcg ctcgagcagc tcttcgagct tgtcgagccg
     5161 cgcgctccag aaggcggcgt agaagctgag ccaagccacg agtggcgcca gcgccttcgg
     5221 attggcgctg tagtgcgttt cgcgtccggc gcgccgatcg cgtacgaggc cggccttctt
     5281 cagcacgccc agatgcttgg agactgccgg ctgggatacg ccggcctcgg cggtgagcgc
     5341 acggacggac tgctcacctc cacggctcag ccgctcgaag atgccgcgcc gggtcgggtc
     5401 ggcgagcgca gcgaaaagat gatccggcgg aacgtgcatc aatgatccat aactcatcgg
     5461 ttatgggtca tgcatagccg attggttatg agttggtcaa gtggctttaa ccgccgcttg
     5521 ggcttacctg aaccggtcga cccggtacgg ccacggatcg gtgtaggtcg gctctcccgt
     5581 catcatctcg gcgagcaagc ggcccgtggc ggggcccagc gtgaagccga ggtggtggtg
     5641 accgaaatcg gcccacagcc cgcgatgtcc tgggacgggg ccgatcatgg gcagcatgtc
     5701 gggcaggcag ggccgggcac ccatccatgg cttgggctcg acgcgatccg cgagcgggaa
     5761 cagctcgcgc gccaccggct cgagcctatc gagctgcacg ggggtgcggg gcgcgtcgcg
     5821 aggggcaaac tcggcgccgg tcgtcaggcg aatgccgcga gtcatcggcg ccagcacgaa
     5881 accgttctcg atgtcgaaca tcggcccgtt gagaaccgca tcgccgcgcg gccggaaatg
     5941 gacgtgatag ccccgcttga cgcccagagg cacgcggatg ccgagcggct tcaggacgtc
     6001 gtccgaccac ggcccgagcg cgatgacggc ttcgcgcgcc tttacaggcc catcgacggt
     6061 gtggacctgc cagccatcca tcgtggcttc gagtgtgcgc gcgtcagcgg tgatgaactt
     6121 gccgccgcgc ttcacgaaga gatcggcgta ggctttgccc agggcgccgg gatcggcgat
     6181 gctgaccggg tccgtcatca gcaggccgcc cacgacgttg cccgtgaggt acggctcgcg
     6241 cttgcggatt gcggccggat cgagctcctc gaagttgatt ccgaactcgc gccggatttc
     6301 ctcctgctcg cgggtatact cggccagccg ctcgtgcgtg cggaagatct tgaggtagcc
     6361 cgtctcgcgg atcatgtcgg tgacgcccgc gtcttccatc agcgcgcggt gctcgatgat
     6421 gcagcgctcg acgagggggc gatttgcccg ggcagaggct gccagccgcg ccggcgtgga
     6481 atagcgccag tacttgaaga gccagacggc gttcgagggc agcgcgctcc agtggtggtg
     6541 cgcctcgggc agcatattga gcgcgtagcg ccacagcttc ttgatgtcgc gcgggaagcc
     6601 gtacggcacg atgccttcgc gctggatcag gcctgcgttg ccgtacgagg tctcctcggc
     6661 tgcgccgcgg cggtcgacga gcacgacgga gcggccgcgc ttctgcaagt gcagagcggc
     6721 ggatacgccg accatgccgg cgccaaggac gagaacatcg gaggtcatgg agctcgtgaa
     6781 ccgatcgcga ttgttacgac tgaaagctca gcgcatctgg ctgcattgat gcccaccctg
     6841 tcgtctgcta ggccagcgcc tgtcaatagg aggtagagcc gcatgatccc ccgctacagc
     6901 cgcccggaga tggtccggat ctgggcgccc gagacgcgtt tccgcatctg gttcgagatc
     6961 gaggcccacg ccgcaacggc catggccgat ctcgggatca tccccaagga agcggcccag
     7021 gcggtctggg agaagggctc ggcggccgaa ttcgacgttg cccgcatcga cgagatcgag
     7081 cgcgtcacga agcacgacgt catcgcgttt ctgacgcatc tggcagaaca tgtggggccg
     7141 gaggcgcgct ttgtccacca gggcatgacg tcctccgacg tgctcgacac gtgcctcaac
     7201 gtgcagctcg tgcgtgcggc agacattttg ctggccgatc tcgaccgcgt gctggcggct
     7261 ctcaagaagc gggcgttcga gcataagaac acggtgacga tcggacgcag ccacggcatt
     7321 cacgccgagc cgactacgtt cggcgtcaag ctggcctacg cattcgccga gttctctcgc
     7381 gcgcgccagc gcctggagca cgcccgttcg gaagtgtcga cctgcgcgat atcgggagcc
     7441 gtcggcactt tcgccaacgt cgacccgcgg gtggaggagt atgtcgccaa gcagctgggc
     7501 ctgacaccag agcccgtttc aacgcaggtc atcccgcgcg acaggcacgc catgtatttc
     7561 gcgacgctgg cggtcatcgc atcgtcgatc gagcgactgg ccatcgagat ccggcacctg
     7621 cagcgcaccg aggtgctgga ggccgaggag tacttttccg aaggccagaa gggctcctcg
     7681 gccatgccgc acaagcgcaa tcccgtgctg acggagaacc tgacgggact tgcgcgcatg
     7741 gtgcgcgcct atgcgctgcc ggccatggag aacgtggcac tctggcacga gcgcgacatc
     7801 tcgcattcct cggtcgagcg gatgatcggg ccggatgcga cggtgacgct cgatttcgct
     7861 ctcaaccgcc tggccggggt gatcgagaac ctcgtcgtct acccggagaa catgcagaag
     7921 aacctcgacc ggctcggcgg gctcatccat tcgcagcgcg tgctgctggc gctgacgcag
     7981 gccggcgtct cgcgcgagga cgcttatcgc ctcgtccagc gcaacgccat gccggtctgg
     8041 cgcggggagg gggatttcct gagtcttctc gctgccgatc ccgaggtgag cgccaagctg
     8101 tcgcgggcgc aactcgagtc tctcttcgac ctcggctacc ataccaagca cgtcgacacc
     8161 atcttcaggc gcgtgtttgg agagagctga gcattcgatg cgcacatccg aagtcgacat
     8221 tctgctcgtg cccgactgga ccggcgctgg cccggatcac tggcagtcgc gctgggagcg
     8281 gagcctgagc acggcgcgca tcatctctca gggtgacttc gatacgccgc gaaaggacga
     8341 ctgggtcggc cggatcgtcg aggcggtgcg gaaggcaagg cggccggtgg tgctggtggc
     8401 gcatagctgc ggcgttgccg cggtggcgca cgcggcgcca tttctggcgg ggctcgacgt
     8461 agcgggcgcc tacctcgtcg ctccagcgga cctcgagcaa acggaggact ggccggtggc
     8521 gcgcggcgac tttacgccga tgccgcttga tcgccttccc tttccggctg tcctggtcgc
     8581 ctcgtcttcc gatccatatt gctcgatgga gcgggcgcgc acctttgccg acgcctgggg
     8641 cgcgacgctg gtgccggccg gcgatgccgg tcatatcgat gccgcatcag gccacggccc
     8701 ctggcccgaa gggctgatgc ggttcggcct gttcctgagc cggcttggac ctgcgaagcg
     8761 aaagctcgac agctgatgct gaagtggtgc gagggtagaa cctcgcgcca cccgcacacg
     8821 ggatccggga tcgagaggcc agcctggatc ttccgtcatt cctggcgcag cttttcaccc
     8881 gagaggcacg cgcctttgtt gggcgtgtgc gaatcgcgta ttacaacttt acggtgtgtc
     8941 cagcgtccgt gttccaactt caggccgagg aatagacgca atgcgcacat acgagaagcc
     9001 gacgctcgtc agaggcgtca agctgcagga tgtcaccgca tcgagctttg tctcgccctt
     9061 cttccttgag aaggaaaaag tcgttctcta agacttgctg cctccgagag cctttaaatg
     9121 ctggctgctc gctctgggca gccatttttt gcggcttcgg ggctaaaacg aaaagtggcg
     9181 gctcaagctg ccgggctaca atacaaaccc tggtgaaagc gctggaaacg gtgggcgtta
     9241 ctgggattga accagtgacc ccacgcgtgt ggagcgtgtg ctctcccgct gagctaaccg
     9301 cccgacccaa ctcgggcttt tcgcggggtt cggacatcat tcagcatgac gtgtagtcgc
     9361 accgctcgtt tgagcgcagc tcaagcggcg agcgcgattc ggtggtgggt tggcggccgc
     9421 ggctcggtga ggaaggagcc ggctgttgag ggttgctgcg gagccgtcgg ggaagatggg
     9481 cgcaaatcaa gttatcgtcc tgaacggcca agcgagcaag gcgacccgcc tgcgttcgaa
     9541 aaaatccacg ggctttgaac cttcaccggc cacgccgcga cagatctgtt gccggcgcaa
     9601 aagcgtgctg gccgccgggc ccgaaacgaa accgtcgaac gcaaatcaga ggaattgagc
     9661 catgatcaat cacaccgttc tggccggcgc tttcgccgct gctctcctta gcctcgcagc
     9721 tgcggatgca gcagttgccg gcagcaaggc gcaagccatc aaccccgaat gcccgaacaa
     9781 gtacgccgtc tgcccaccca agctgaaggc gaaggaaaag ccggattcgg tgccaatgaa
     9841 agccgtcccg gcttcgaaga ccgtcaagcc aaagaaggag cgcagccgct gagcgaaata
     9901 agcaacagaa ggcggcttga ccaagtctcc ctgcgcataa gcggggaggc ttcccatttt
     9961 cgacggagcc aaggagcaga tggtgggcgg tactgggatt gaaccagtga ccccacgcgt
    10021 gtgaagcgtg tgctctcccg ctgagctaac cgcccgaaaa ccagggaagt gaaccggact
    10081 tatgggagag agccggtccg gctgtcaagc cttcaggcga ggggtgaagc cctgcgcgat
    10141 cttccggaat tccggaagca atgccggcca ttcggctgcg ggggcccgac cgacgatgcg
    10201 gtaatagccg ccgtccttgc ggcgcaaaat cacctgatag atcccggtat cgaggcccgt
    10261 tccgctcggc ggcacgacct gctcgatgcc ttcgagcccc gctacctcga ggggccggct
    10321 ctcgaggcct tgcggctccc ggccacccgc gagggccgcg atcccggcgc ggccggtggc
    10381 gtccaggtcg tcgatatggg ggccatcgag cgaggccgcg atgacgaggc tcggctgctg
    10441 actggcggca gcgccattcc cgcccgccgg ccggtcctcg atcgcgaaga aataggactg
    10501 gccgatgaag gcgccggtat ccctgaacgg ccccatgtag cccagctcga acggcttctc
    10561 gccggccttg ggatcgatcc gcgccgaggc caggatctgc tcgaaggtgc gcggctcggc
    10621 gctgccgttc tcgaaagagc tgcggggcac gttgatgctg acgagagccg tcgcagacgg
    10681 ctcccggaac agcaggatgt atttcatata agggccagat gccgtctgct gctcgcccgt
    10741 cacgtagaca
//
LOCUS       DASVDU010000027         4217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_918871, whole genome shotgun sequence.
ACCESSION   DASVDU010000027 DASVDU010000000
VERSION     DASVDU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918871"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VF226_02110"
     CDS             <1..1215
                     /locus_tag="VF226_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX7072807.1"
                     /translation="FVFFEVTLAASYGLLLHGGGAFRVRSGLHYIAINLAASALFLIG
                     VSLVYGVTGTLNMADLAVRIPQVPADDRMLLQAGAGILGVAFLVKAGMWPLGFWLPPA
                     YMAAAAPVGAIFVILTKVGIYVLLRLTLLLFSGFSGQLAGFGSTALLYGGLATLAFAT
                     SGVLASQAMGRLAGYSVLISSGTLLAAAGVGDATVVSGALFYLVSSTFACAAFFMLIE
                     LVERAREPGADLLAVTAEAFGEEREETEEPEDEEVGVVLPGALAVLGVSFACIALLLA
                     GLPPLSGFIAKFAILKGLLDVEGLAELGGVPASTWLLMALVILSGLATLVAMTRTGIR
                     TFWAPLEEVVPRVLVMEMAPILALLAICLAMVVGGGAVVSYTDAAARSLHDPASYVGG
                     VLDTSLPVGDGR"
     gene            1212..1697
                     /locus_tag="VF226_02115"
     CDS             1212..1697
                     /locus_tag="VF226_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX7072808.1"
                     /translation="MSRVLPYPLLTAGLLLMWLLLTSFSLGQLLLGALIATAASRAMA
                     ALQPSKPRLRRWRLIPRLAGIVAADILRSNAAVFSIIVRGRHRERVSGFVRIPLELRD
                     KTALALLACIITSTPGTAWIEFAPDTGILVIHVLDLVDEDEWVALIKNRYEALLMEIF
                     E"
     gene            1694..1975
                     /locus_tag="VF226_02120"
     CDS             1694..1975
                     /locus_tag="VF226_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEX7072809.1"
                     /translation="MIVDILLWSVTAAQVMLVAAMACYAFRVVRGPRAQDRVLSLDAM
                     YVCAMLLALTVGIRTGSDVYFEAALIIGVLGFVSSVALAKFLMRGEVIE"
     gene            1972..2370
                     /gene="mnhG"
                     /locus_tag="VF226_02125"
     CDS             1972..2370
                     /gene="mnhG"
                     /locus_tag="VF226_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX7072810.1"
                     /translation="MSHAADLPVWAALLTAALLLAGATLTLAGAIGTLRFRSFYERVH
                     APTLGTSWGTGAMALASIVFFSALGSRLVLHELLIAVFVTVTTPVTLMLLARAALYRD
                     RAEGAAGVPAQSELRRPNGNGEASHESGAI"
     gene            2721..3023
                     /locus_tag="VF226_02130"
     CDS             2721..3023
                     /locus_tag="VF226_02130"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX7072811.1"
                     /translation="MSAIEHTTSQAREAVTFRKPLTVVARIVLRVLRRWQRNRVIDEL
                     WRLDDRQLRDIGIKRGDIPGLVDRFFREKRELDRILVHQAPVDHVRSVAAQLRRAA"
     gene            3517..4119
                     /locus_tag="VF226_02135"
     CDS             3517..4119
                     /locus_tag="VF226_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7072812.1"
                     /translation="MSAAVMSAGSFPASIRRGWRPPRAASPAAEKLRSATGAANAPDE
                     PATSAGNVGDILSPDGVLLDLQARSKRSVLALIATQLAMQAGLRSGEVQAALLRRERL
                     GPTFIGNGVALPHARIKGLSRPEVVLARLRDPVSPRTPNGEVVDLVLAILWPAHQSGF
                     VPTLARSWRLLRRPGLADDLRRSGSAEEICALIRWAEQRP"
BASE COUNT          620 a         1394 c         1390 g          813 t
ORIGIN      
        1 ttcgtcttct tcgaggtcac gctcgcggcg tcctacgggc tgcttctgca cggcggcggc
       61 gctttcagag tacgctcggg tctgcactac atcgccatca atcttgctgc ctccgcgctg
      121 ttcctgattg gcgtcagcct cgtctacggg gtaaccggga cgctcaacat ggcggatctc
      181 gccgtgcgga tcccccaggt ccccgccgac gacaggatgc tgctccaggc gggtgcaggc
      241 attcttggcg tcgccttctt ggtcaaggcg ggcatgtggc cgctcggctt ctggctgccg
      301 ccagcctaca tggcagctgc cgcgccggtc ggcgccatct ttgtcatcct gacaaaggtc
      361 ggcatttacg tgctgcttcg gctcaccctg ctgcttttca gtggcttttc cggccagctg
      421 gccggcttcg gtagcaccgc attgctgtat ggtggcctgg cgaccctcgc ctttgcgaca
      481 tcgggcgtcc tcgcttcgca ggccatgggg aggctcgccg gatacagcgt cctgatttca
      541 tcgggaacgt tgctcgcggc cgccggcgtc ggggacgcca ccgtcgtgtc gggcgccctg
      601 ttctatctcg tcagctcgac tttcgcctgc gccgccttct tcatgctgat cgagctcgtg
      661 gagcgcgcgc gcgagccggg tgcggacctg ctggccgtca cggcggaagc gttcggtgaa
      721 gagcgcgagg aaaccgagga gcccgaggac gaggaagtcg gcgtggtcct gccgggagcg
      781 ctcgccgttc tcggcgtctc cttcgcgtgc atcgcccttc tgcttgccgg gctgccgccg
      841 ctgtcggggt tcattgcgaa gttcgcaatt ctgaaagggc tgctcgacgt cgaggggctt
      901 gccgaactgg gaggggtgcc tgccagcacc tggctgctca tggcactggt cattctgtcg
      961 ggactggcga cgctggttgc gatgacgcgc accggcatcc ggaccttctg ggcgccgctg
     1021 gaggaagtcg tcccgcgtgt cctggtcatg gagatggcgc cgatcctggc actgctggcc
     1081 atctgcctgg caatggttgt aggcggcgga gcggtcgtca gctacaccga cgcggccgcc
     1141 cgttccctgc atgaccctgc gagctacgtc ggcggggtgc tcgacacgag cctgccggta
     1201 ggagacggcc gatgagccgc gtcctccctt atcctctgct gactgccgga ctgctgttga
     1261 tgtggctgct gctcacgagc ttttcgctcg ggcagctgtt gctcggggcg ttgatcgcga
     1321 cagcggcttc ccgggccatg gccgcccttc agccttcgaa gccgcggctg aggcggtggc
     1381 gtctcattcc gcgactggca ggcatcgtcg cggccgacat attgcggtcc aacgccgccg
     1441 tgttcagcat catcgtcagg ggtcggcacc gtgagcgcgt ttcgggcttc gtgcgcatcc
     1501 cgttggagtt gcgggacaag actgcgctgg cgctgctcgc ctgcatcatc accagcacgc
     1561 ccggcaccgc ctggatcgaa ttcgcgcccg acaccggcat cctcgtgatc catgtgctgg
     1621 acctcgtcga cgaggacgaa tgggtggccc tgataaagaa ccgctatgag gcgctgctga
     1681 tggagatttt cgaatgatcg tcgatatcct cctctggtcg gtcacggccg cgcaggtgat
     1741 gctggtcgcg gcgatggcct gctacgcctt tcgcgtcgta cgcgggccgc gtgcacagga
     1801 tcgggtgctc agcctcgatg cgatgtatgt ctgcgccatg ctgctggcgc tcacagtcgg
     1861 catccgcacc ggcagcgacg tgtatttcga agccgcgctg atcattggcg tgttgggctt
     1921 tgtttcaagc gtggcgctcg caaagttcct catgcgtggc gaggtgatcg aatgagccac
     1981 gccgccgacc tgcccgtctg ggcggccctt ctcaccgccg cactgctgct tgcaggcgcc
     2041 acgttgacgc ttgccggcgc catcggcacg ctccgcttcc gcagcttcta cgagcgggtg
     2101 cacgcaccaa ctctgggaac gagctgggga accggcgcga tggcgctcgc ctccatcgta
     2161 ttcttctcgg cccttggctc gcgtcttgtc ctgcacgagc tgctgatcgc ggtattcgtg
     2221 acggtgacga cgccggtgac cctgatgctg cttgcgcgcg cggcactcta ccgcgacagg
     2281 gcggaggggg cagcaggcgt gcccgcgcaa tcggaactgc ggcgcccgaa cggaaacggc
     2341 gaggcgagcc acgagagcgg cgcgatctga acggccgcct gccagcagct caggccgccg
     2401 gtgccacgca tcggggctgc acatggcccg tgcgcccaaa ggccgcacgc ccgcgcatcc
     2461 gttgattctc aaggcctggc ttcccatatc cgagccatgt tcagtccagg tcaacacaag
     2521 aagcatctgg tcgtctcggc gtccctcctg acgcgctcgt aaccccgagc ggcacggcgc
     2581 gttcgcctgc ccgctcgggg aaacttttca gaacgaaatc gcatgcctct tccaaccgag
     2641 ccctcggctg gcggaacgca catcaacgag cctcgcactg tcgggcgggt cgccatcgcg
     2701 cgaacgaaag gagccacgcc atgtccgcaa tcgaacacac cacctcacag gcaagagaag
     2761 ccgtcacctt ccgcaaacct ctcacagttg tcgcccggat tgtgctgcgg gtgctcaggc
     2821 gctggcagcg caaccgcgtg atcgacgagc tgtggaggct ggatgacagg cagctcaggg
     2881 acatcggcat caagcgcggc gacattccag gcctggtcga caggttcttt cgcgagaagc
     2941 gggagctcga ccgtatcctc gtccatcagg cgccggtcga ccatgtgcgc agcgtggcgg
     3001 cgcaattgcg cagggccgcc tgagccgctg tgtcgcgaag gaggcgatga tggatgcgcc
     3061 gaccaagatg gtcgtagccg ccggactgtt cggcctgatg ctcatcgcgt tcgtgctgct
     3121 gtgacggcgg cggatggggt tgaggtaggc ccgatacccg gttaggaacg gtcatgtgct
     3181 tggagacgtt catcaggatt acgaggcttg ccggcgaagg tcgcgacctt gcccggagca
     3241 ggctcgttaa ccctctgtcc cgcacctaat cctgctccgg ggcttccgaa cagcaaagcg
     3301 gttcattcct gcggcgccac gcgccgcatc aatggagcgt gacatgagat acttccgatc
     3361 gagacgcgtg tgcctgtcct gcagtcgtct gttgccacga gcggcagccc ggtgcccctg
     3421 gtgcatcgac tccgtagtcg tgccagctac gcacgctgat caacccgtgc cccgcattgc
     3481 gccgtccgga cggacgacgc ccccgcaggt gctgcgatga gcgcagccgt catgagcgcc
     3541 gggtcgtttc cggcaagcat ccgtcgcggc tggaggccgc ctcgcgccgc gtcgccggct
     3601 gcagagaagc ttcggtctgc cacaggcgct gccaatgcac cggacgagcc tgcgacatcc
     3661 gctgggaatg tgggagacat tctttctccc gatggcgtgc tgcttgactt gcaggcgagg
     3721 agcaagcgct cggttcttgc gctgattgcc acccagctcg ccatgcaggc cgggctccgc
     3781 agcggcgagg tccaggccgc gctgctgagg cgcgagcgcc ttggtccgac cttcatcgga
     3841 aacggtgttg ccttgcctca tgcgcggata aagggcctaa gcaggcccga ggtcgtgctg
     3901 gcgcgcctgc gcgaccctgt ctcccctcgc accccgaacg gggaagtcgt ggacctggtg
     3961 ctggcaatcc tgtggccagc gcatcagtcc ggcttcgtgc cgactttggc ccggtcctgg
     4021 cggctgttgc ggcgcccggg acttgccgac gacctcagga gatcgggatc ggccgaagaa
     4081 atctgtgcgc tgatccgctg ggcggagcag cggccatgac ccgaccggca actggcttga
     4141 gcgccatcat cgcctggcgt gccgctgccg tcacgacaaa ccctgtttcc ccctccggtg
     4201 cgaaccgatg gcgggcc
//
LOCUS       DASVDU010000028         6081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_725736, whole genome shotgun sequence.
ACCESSION   DASVDU010000028 DASVDU010000000
VERSION     DASVDU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725736"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..1300
                     /locus_tag="VF226_02140"
     CDS             488..1300
                     /locus_tag="VF226_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072813.1"
                     /translation="MELTVTLSPPGSVSQGVQVRDGAEVVVKMINNAGGVLGRPLELV
                     VEDTQGIPEKARAAVEKLITRDKVVAVTGEHQSSAALAGIEVAHRYNVPYVNTNGWSD
                     VIREKGYPQVFNPGNFNSRVAIAAAETIAALGAKNVLAFPENTDYGVGLAKLAPNSGR
                     VLLDGNSIEGLAPAELARRGVMRMFQLTRVFPRMSVIDNLMVAGCALGLLQSEAEERA
                     WQVVEDLSLTHVAALDWPGTGKGTNSGPPWPVLVKVRSPLAHASKADRHSLY"
     gene            1346..2572
                     /locus_tag="VF226_02145"
     CDS             1346..2572
                     /locus_tag="VF226_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX7072814.1"
                     /translation="MQDQNSRLGRYIETVAGGEMVRAYVPPPLPPSPPLQLQSLLPRL
                     SAADQAIGRLDGISILLPDRSLFLYMYVRKEAVLSSQIEGTQSTLSDLLRFEASAASG
                     TPVDDVREVSNYVDAMMYGLERLATLPISLRLIREMHKRLLQSGRGRTQNPGEFRRSQ
                     NWIGGTRPGNAMFVPPPHQEVVTCLADLEKFIHDQSPGFPPLIKAGLIHVQFETIHPF
                     LDGNGRLGRLLITLYLCSQKVLQQPLLYLSLYFKKRRADYYRLLQEVRERGAWEAWLE
                     FFLDGVAETANEAFNTATRIHDLIRNDRERISSEAERSSSALRVHEIMQTSPFLTTSK
                     AVQKTGLTKPTINAALDQLLKLGIIEEVTKKRRGRVYAYKAYLDILNEGGEPLDNTGT
                     MKAASVVGTGQGRLNK"
     gene            complement(2730..3899)
                     /locus_tag="VF226_02150"
     CDS             complement(2730..3899)
                     /locus_tag="VF226_02150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7072815.1"
                     /translation="MLQLAQNLDRLGTETAFEVLARAAELQRQGRDIINLGIGQPDFQ
                     TPNHIVEAAVKALRDGHHGYTPAQGILPLREAVAADLKRRHDVEVNPDNIVIMPGGKP
                     TMFFSILMFGEPGTEIMYPDPGFPIYRSMIEYTGATPVPIALKEENGFAFSAEEVLTQ
                     ITPRTRLIIINSPGNPTGGAVPEEEIKRLISGLERHPHVAVLSDEIYSQILYDGREHV
                     SFLKFPEIRDRLILLDGWSKTYAMTGWRLGFSVWPTNLVEKVVRLCVNCHSCVNAPTQ
                     YAGIAALEGPQDDVLKMVRAFDERRRVIIPMLNQLPGFRCVEPGGAFYAFPNITGTGM
                     TAKELQNKMLEEAGVATVAGTSFGVYGEGYIRFSYANSVENIRAAIQRIGTLLTK"
     gene            complement(4010..4342)
                     /locus_tag="VF226_02155"
     CDS             complement(4010..4342)
                     /locus_tag="VF226_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072816.1"
                     /translation="MELAPLARAPLVAESVAEDSDGLSRLPRDPVTGHHITSSSTGQA
                     NALVPLQAPPLPKGREPARLSPVGITPPAQYWADEFATDVRRHIGGFLIGLAIASAMG
                     IGLYAVLT"
     gene            complement(4584..>6081)
                     /locus_tag="VF226_02160"
     CDS             complement(4584..>6081)
                     /locus_tag="VF226_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072817.1"
                     /translation="GDASVITIESAMLVVLGFLVATLIALAFAPIYRARAMRLTYKHI
                     RRVLPLTESEIQVDRDRLRAQYAIRIHDLETKLEKARLAAARQQVEVNRRDAAVAALE
                     AELAQLRSDLEQNVNARRVLEQTVMDRLPAVEQRLAETRRLLEQRDRELAAITAESGK
                     TVRALDEVMQINAQQRAEIERLHGMIATRGLRSRVGARDSRFESEVALRSELEALRAK
                     TREQESLISRLQTLVTVPSASKRPAQANGGAAGHEQDLELERLRRDLAEAESALKSVN
                     EQAEAGGADSSVRARLQSLENLVENQASKIRELEAALAEYEKGGSDSQSMLKDSKIAM
                     KARIASLQAQVENRTAMVEKLRAELASANERLALQSAQFRDEMRRLRTSAPPPAENVD
                     ENASARRSLAKRIGEANPALAASLRSGSLRSVTKRPSEADPVATQETAEAAGEAAHSV
                     AHASLAQANGSELRPSEVKESAPEHQQAETDGQARTRLLDRIASISKS"
BASE COUNT         1134 a         1893 c         1852 g         1202 t
ORIGIN      
        1 gctcggccat tccgcctccg ccacccctgt tctccatggt gcctccacca ccgccctggc
       61 cgctttgcat acccccaccg cctccgccgc ccctttgtcc gccttgctgc ccgccggcgc
      121 cctgtatccg aatgaccgag ctgctgctca ccccgagctt cgatacggcc gctgaggaca
      181 acgttgcgcc cacgggtggt gaagcgaaca caagcaaggc cgctgctgcg gccaaggaac
      241 atgctgtaga aggtttcata cgacgtctcc cgaagttgtg gttagcgcct gcgcgccggt
      301 attttttctg acgcgtgcct aagtcttccg tagtcgagct tcggcgaaag gccccaattc
      361 aacttgcgag agggtgagat atagggcgca attcttgcgc gacattccga cacatcttga
      421 agcacgcttg tgctattctt gttgtccgcc gctctaggtc gcaccccaaa caagaacacc
      481 gatcaagatc gagctcaccg tgaccctctc gccacccgga tcggtgtccc agggcgtgca
      541 ggtgagggat ggcgccgaag tggttgtgaa gatgatcaac aatgccggcg gcgtcctcgg
      601 gcggccgctc gagctggtcg tcgaggatac gcaaggcatt cccgaaaagg cccgcgcagc
      661 ggtcgagaag ctgatcacgc gcgacaaggt ggtggcggtg acgggcgagc accagagctc
      721 cgcggcgctc gcaggcatcg aggtggccca ccgctacaac gtgccgtacg tcaacaccaa
      781 cggctggtcc gacgtcatcc gcgagaaggg ctacccgcag gtcttcaacc cgggcaattt
      841 caacagccgc gtggccatcg ccgcggcgga gacgatcgcg gctctcggcg cgaagaacgt
      901 gctcgctttc cccgagaaca ccgactacgg cgtgggtctc gcaaagctcg ccccgaattc
      961 cgggcgcgtg cttctcgatg gaaactcgat cgagggcctc gctccggcgg agctggcgcg
     1021 ccgcggcgtg atgcgcatgt tccagctgac gcgtgttttt ccgcggatga gcgtcatcga
     1081 caatctcatg gttgcgggtt gtgcgctggg gcttctgcag agtgaggccg aggagcgggc
     1141 ctggcaagtc gtcgaggacc tgtccctgac tcacgtcgct gcactggact ggcccgggac
     1201 tgggaagggg accaacagcg gccctccctg gccggtgcta gtaaaggtgc gttcccctct
     1261 ggcgcacgca agtaaagcag atcgccattc cctttactaa cgagatgtgt tagttaaacg
     1321 tgctttccta gcgtgcgaca gggccatgca agatcagaac tcccgactag gtcgctacat
     1381 cgaaacggtt gctggcggag agatggtgcg agcgtatgtg ccgccaccac ttcctccgtc
     1441 tcctccgctc cagcttcagt cactgttgcc gagactcagt gcagcagacc aagctattgg
     1501 gcgattagat ggcatttcca tccttctccc tgacaggagc ctcttcctct acatgtatgt
     1561 acgaaaggag gcagtcctgt cttcgcagat agaaggcact caatcgacct tatcagatct
     1621 actgcgcttt gaagcatcag cagcttccgg gacgccagtc gacgatgttc gcgaagtctc
     1681 gaactatgtc gatgccatga tgtatggcct agaacgccta gccacgttgc caatctcact
     1741 ccgccttata cgtgaaatgc acaagcgact tctgcaaagc ggacgtggga gaacccaaaa
     1801 tcctggtgag tttcgacgct cacagaattg gatcggaggg accaggcctg gcaacgctat
     1861 gttcgtacct cctccacatc aagaggtagt cacttgtctg gctgacctcg aaaagttcat
     1921 ccatgatcag tcgcctggtt tccccccctt gataaaagct ggcctcatcc acgtccagtt
     1981 tgagactatt catccgtttt tggacggtaa cgggcgtctc ggcaggctgc ttataactct
     2041 gtatctttgc tcgcagaaag ttcttcaaca gccacttcta tatctcagct tatatttcaa
     2101 aaagcgacgt gccgattact atcggctcct tcaggaagtg cgcgagaggg gagcatggga
     2161 ggcgtggtta gaatttttcc tcgacggggt cgctgagact gctaacgaag ccttcaacac
     2221 tgccactcgc atacatgatc taattaggaa cgatcgtgaa cgcattagct ctgaggcaga
     2281 acgctcttca tcggcgttac gcgttcacga aataatgcaa acttcgccat ttttgacgac
     2341 ctccaaggct gtacaaaaaa cgggtttgac gaaaccgact atcaacgctg cattagatca
     2401 gctgttgaaa ctcggcataa ttgaagaagt tacgaagaaa cgtcgaggac gcgtctacgc
     2461 atacaaggca tacctcgaca tactaaacga gggcggggaa ccacttgaca acacaggcac
     2521 aatgaaagcg gcatcggttg tcggaacagg ccaggggaga ttgaacaaat aaggtgggaa
     2581 ccgcatcgtg ttcccgcgct ggtcgcgaaa cggacggacc tcgcctgcaa cccgcagtgg
     2641 tctgagcgcc ttcccaactc gtttgatcag atcgctccga cctaagatca ctcgcaaccc
     2701 tccccatccg gggagggctg acgaacgcct cacttcgtca acaacgtccc aatccgctgg
     2761 atcgcggcgc ggatgttctc gacggagttg gcgtaggaga agcggatgta gccctcgccg
     2821 tagacgccga agctggtgcc ggcgacggtc gcaacgcctg cttcctccag catcttgttc
     2881 tgcagctcct tcgccgtcat gccggtgccg gtgatgtttg ggaacgcata gaaggcgccg
     2941 ccgggctcga cgcagcggaa gccggggagc tggttgagca tcggaatgat gacgcggcgg
     3001 cgctcgtcga aggcccgcac catctttagc acgtcgtcct gcggcccttc gagcgcggcg
     3061 atgccggcat attgcgtcgg cgcattcacg caggaatggc agttcacgca caaccgcacg
     3121 accttctcga cgagattcgt tggccacacg ctgaagccca gccgccagcc cgtcatcgcg
     3181 taggttttcg accagccgtc aagtagaatg agccggtcgc gaatttcggg gaacttgaga
     3241 aagctcacgt gctcgcggcc gtcgtagagg atctggctgt agatctcgtc cgacagcacc
     3301 gcgacgtgcg ggtgccgttc aaggccactg atgaggcgct tgatctcctc ctccggaacg
     3361 gcgccgcccg tcggattgcc tgggctgttg atgatgatga ggcgcgtccg cggcgtgatc
     3421 tgcgtcagca cctcctcggc gctgaaggcg aatccgttct cctccttcag cgcgatcggc
     3481 acgggtgtgg cgccggtgta ctcgatcatg gaccggtaaa tggggaagcc cggatcgggg
     3541 tacatgatct cggtccccgg ctcgccgaac atgagaatgg agaagaacat cgtcggcttg
     3601 ccgccgggca tgatgacgat attgtccggg ttgacctcga catcgtggcg acgcttcagg
     3661 tccgcggcga cggcttcgcg gaggggcagg atgccttgcg ccggcgtgta gccgtggtgc
     3721 ccgtcccgca gcgccttcac ggcagcctcg acgatgtggt ttggcgtctg aaagtcgggc
     3781 tggccgatgc cgagattaat gatgtcgcgc ccctgtcgct gcaactcggc ggcacgtgcg
     3841 agcacttcga acgcggtctc tgttcccaac cgatcaagat tctgagcgag ctgcagcatg
     3901 tgactccccg tgcttcaaag atgtgaagaa tgttgcacga ggcgggcgcg aacggaagtg
     3961 cccgcgccgc aagggtgtca gctcctgcac gcaggtggag tgcagccgtt caggtcagca
     4021 cggcgtagag tccgatgccc atggcggagg cgatcgcgag cccgatcaga aatccgccga
     4081 tgtgccggcg cacgtccgtc gcaaactcat ctgcccagta ctgcgccggc ggtgtgatcc
     4141 cgaccggaga caggcgcgcc ggctcccgcc cctttggcag tggcggcgcc tgaaggggga
     4201 caagtgcgtt tgcctgtcct gtggaagagc tggtgatatg atgccctgtc accggatcac
     4261 gtggaagccg gctgagaccg tcgctgtcct ccgccacact ctcggccacc agcggagctc
     4321 tcgccagcgg agccagctcc accagacgct cagccagacg cggcctggcg gcagccgatg
     4381 gatttgatcg aatagatcgc gaagcacggg cgactttcgc ttcattcaga tcagagatgt
     4441 gcttggacat gctcttggac ccgcggtctg ttgcgaacga cgcctcccct gacagttgat
     4501 ctaactgcct ggagctcgcg cggaaagcga aaatctcaga aaataagtaa caaaattaag
     4561 gccagtggaa cgcccgtccc aagtcaggac ttgctgatgc tggcgatcct gtccagcagt
     4621 cgcgtcctgg cctgaccgtc agtctccgcc tgctgatgct caggggcgga ctccttcact
     4681 tccgacggcc gcagctcgct tccgttggct tgcgcgagac tggcgtgagc gacggaatgg
     4741 gcggcctcgc ctgccgcctc tgcggtttct tgagtcgcaa ccggatcggc ttcggacgga
     4801 cgcttcgtga cgctgcgcaa cgacccggac cggagcgagg ctgcgagcgc aggattggcc
     4861 tcgccgatcc gtttcgccag cgaccgccgt gccgatgcat tttcgtcgac gttttcggct
     4921 gggggcggcg cagacgtccg gagccggcgc atttcatccc ggaactgggc ggactgcagg
     4981 gccaaacgct cgtttgcgga cgcgagctcg gcgcgcagct tttcgaccat cgcagtccgg
     5041 ttctcgacct gggcctgcag agaagcgatg cgcgccttca tggcgatctt gctgtccttc
     5101 agcatcgact ggctgtcgct gccgcccttc tcgtattcgg ccagagcggc ctcgagctcc
     5161 cggatcttcg aggcctggtt ctcgaccagg ttctctagcg actgcagcct tgcccgcacc
     5221 gaactgtcgg ccccgcctgc ctccgcttgc tcgttgaccg atttgagagc cgattcggct
     5281 tcggccagat cgcgccgcaa ccgctcgagc tccagatcct gctcgtgacc ggccgcgccg
     5341 ccattggctt gggcgggccg tttcgaggct gacggcacgg tgacaagcgt ttgcaggcgc
     5401 gaaatgaggg attcctgctc gcgcgttttt gcacgcaagg cctccagctc ggacctgagg
     5461 gcgacctcgc tctcgaaccg gctgtccctt gctccgacac ggctgcgcag cccgcgggtg
     5521 gcgatcatgc cgtggaggcg ctcgatctcg gcccgctgct gggcattgat ctgcatgacc
     5581 tcgtcgaggg cgcggacagt cttgccggac tctgcggtga tggccgcaag ctcgcgatcg
     5641 cgctgctcca gcagccgccg cgtctccgcg aggcgctgct ccaccgccgg caggcggtcc
     5701 atgacggtct gctcgagcac acggcgcgcg ttgacgttct gttctaggtc cgaacgaagc
     5761 tgcgccagct cggcttccaa ggcggcgacc gccgcatcgc gcctgttgac ctcgacctgc
     5821 tggcgagcgg ccgccagtct cgccttctcc agcttggttt cgaggtcatg gatacggatg
     5881 gcgtactggg cgcggagcct gtcgcgatcg acctggatct cgctctcggt caaaggcagg
     5941 acgcggcgga tgtgcttgta ggtcaggcgc atggcccgcg cccggtaaat gggcgcaaac
     6001 gccagtgcga tgagcgtggc caccaggaag cccagtacca cgagcatggc cgactcgatc
     6061 gtgatcactg aggcatcccc g
//
LOCUS       DASVDU010000029        16001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1501750, whole genome shotgun sequence.
ACCESSION   DASVDU010000029 DASVDU010000000
VERSION     DASVDU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501750"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3400
                     /locus_tag="VF226_02165"
     CDS             <1..3400
                     /locus_tag="VF226_02165"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX7072818.1"
                     /translation="LAGVVRAHTSAKEAGLRLIVGARLDLQDAPSLLCLPTDRAAYGR
                     LCRLLTLGQRRAEKGQCTLFLDDVAAHREGQIFIALPPEDWDWREAISQEKQSHGATV
                     VPFPARAQQTPSPRRGEGWGEGGPAVAVSGIGPTPKSSPHEASERPFETELRRIKAAL
                     APAPVYLAASHRYQGDDRARIAALSRLAERAGTLLVATNDVLYHVPERRPLQDVLTCI
                     REGTTIREAGLKLAPNAERHLKGPSEMARLFAGYEAAVARTLEIAAACRFSLDELVYE
                     YPDEPVPPGKTPQSHLEELTWEGARWRFPEGIPEKVAALIRRELGLIAQLNYAPYFLT
                     VYDIVKFARSQNILAQGRGSAANSVVCYCLGITAVNPTEIDVLFERFVSPERKEPPDI
                     DVDFEHERREEVIQYIYARYGRERAGLAATVISYRARSAVREVGKAMGLSEDTVAALA
                     GMVWGISGGTVPESHVREAGLDPRDPLLATVLALADELIGFPRHLSQHVGGFVLTRSL
                     LSEVVPIGNAAMEDRTVIEWDKDDLAALGLLKVDVLALGMLTCIHRAFDLIETHYGEH
                     WTLANIPREDSKVYDMLCRADSIGVFQVESRAQMNMLPRLKPRTFYDLVIEVAIVRPG
                     PIQGDMVHPYLRRRSGIEPEVYPSPAPEHGPPDELRQILGKTKGVPLFQEQAMRIAMV
                     AAKFTDAEVNELRKAMATFRRAGTIGKLEHKMVSRMVARGYDPEFAARCFSQIKGFGE
                     YGFPESHAASFAHLVYVSAWIKCHYPAVFAAALLNSQPMGFYAPAQIVRDAREHGVEV
                     RPVDINFSHWDCTLEPVAERGERPVLALRLGLRQVDGLIEDDAQKIVAARTVSAIAGP
                     PSPRPSPHRGERVRPYRDVHDLWARAGVRLATIEKLAAADAFRSVGLDRRQALWEVRA
                     LTAARPLPLFAWSEAREAGAEPAVALPEMQLPEHVVNDYQTLRLSLKAHPVSFLRPQL
                     AGERAIPCAALRNLKDGQWVTVAGVVLVRQRPGSGQGVVFMTLEDETGIANIVVWPKM
                     LERFRKVIMGGRLILVRGRVQRHEDIIHVVATSLADRSDLLLELSEWKEDFNLALANA
                     DEVKRPDPGSARNTGHPRFARHPREERTIPKRLEAVLPKSRDFH"
     gene            3633..5174
                     /locus_tag="VF226_02170"
     CDS             3633..5174
                     /locus_tag="VF226_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEX7072819.1"
                     /translation="MQDNLTAASAEPRTLLLNDRDNVIVAVDGLAPGAVARGVTASSR
                     IPRGHKMAIAKIAKGEPVLKFGQIIGFATEDIAPGEHVHTQNCGFAAFERDYAFCQDA
                     KPEPLLPPEARATFQGFRRSNGRAGTRNYIGIISSVNCSATVVRFIAEEVKRSGLLDD
                     YPTIDGVIPLVHGTGCGIDYNGETFEILKRTTWGYACNPNMAGVLVVGLGCEGFQIAR
                     MKEAYGITESDTFRTMTIQETGGTKKTIAAGVDAIRSMLPIAAKARRETIPASELMVA
                     LQCGGSDGYSGITANAALGLATDILVRHGGTGVLAETPEIYGAEHLLTRRAATREVGE
                     KLVEIIQWWEDYTARARMSMDNNPSPGNKAGGLTTILEKSLGAAAKGGSTTLNAVYRY
                     AEPITAKGFVFMDTPGYDPVSATGQVAGGCNIVCFTTGRGSAYGCKPSPSIKLATNST
                     VYHHMIDDMDINCGDILDGVPLEAKAQEIFETILRVASGERSKSELLGYGDAEYVPWQ
                     IGATM"
     gene            complement(5299..6480)
                     /locus_tag="VF226_02175"
     CDS             complement(5299..6480)
                     /locus_tag="VF226_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7072820.1"
                     /translation="MARISEVAVIEFKFTVANLGLSAESARAVYNIGYKPGGNLELTK
                     YAVVIRSDDGAEGQYVTHWGGTRASMAQTLALAPNLIGRDPDAREEIWNDMKRELRQH
                     DGMGIGPIDIALWDLAGKRFGASVSQLLGGFRTRLKTYASTYHGDRNGGLDSPDAYAD
                     FAERCYEMGYRAFKIHGWHDGDPREEARNVLHVAKAVGDRMTLMLDPANELRTFADAL
                     YVGRACDEGGYFWYEDPLRDCGVSAFAHKKLREMLKTPILQTEYLRGFETKADFLIAG
                     GTDMLRSDPEYDLGITGAMKIAHLAEAFGVDVEIHACGPAHRHCMAATRNTNFYELAL
                     VGPDTPNAVPPVYTCGYSDQLDAIGEDGCVPVPTGPGLGVTYDWEFIRKNQTFAQVFR
                     A"
     gene            complement(6520..7794)
                     /locus_tag="VF226_02180"
     CDS             complement(6520..7794)
                     /locus_tag="VF226_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7072821.1"
                     /translation="MITTLLVVFAVLLVIGTPIAIAMAGSAILLIWVDGIPLTVVAQR
                     VVAGVQSFPLLAIPLFILAGSLMNESGISERLFGFTRALVGHIRGGLAHTAIVGNVFL
                     AGISGSSVADTAATTRVFVPQLQNAGYPKAFGAAVSATAGTLGPIIPPSILMVVYAWQ
                     ANISLGALFVAGVLPGLVMAASMMTAIAILARVHGFRPDGEFSLANVWLQFKRALWAL
                     MMPVLILVGFRMGLFTATEIAGVAAAYALIVGFLIYRSLSLRDLPRILVQTARETAVI
                     LFIVAAATPFSWILGIEQAPQLVVEFVSSISTNPWVVLLILNVALLLAGMVMETLAIM
                     IILVPILLPLLTSLDIDLVHFGIILLVNLVLGQLTPPVGVLLFVVMAMTGLKLTALIK
                     ELWPLLIAVLLALLFITYVPGLSLWLPRVLGF"
     gene            complement(7791..8306)
                     /locus_tag="VF226_02185"
     CDS             complement(7791..8306)
                     /locus_tag="VF226_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7072822.1"
                     /translation="MRAFLRRLDDAAARGEEIFLAAALGLLTVLVVAAVVFRYALHDP
                     LTWSEEFIVLLFGWAIMIGTAYAFHQRNHIVIDVILLFAPRWLRIVLGTLSTIATFVV
                     LAVLVWFGWQYTMREFPNLTPMLGISAAWSILPLTIGCALSMLHVLRRLLDDGPQSAL
                     WVEMISRKTDE"
     gene            complement(8321..9328)
                     /locus_tag="VF226_02190"
     CDS             complement(8321..9328)
                     /locus_tag="VF226_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7072823.1"
                     /translation="MKGFVIKSLALAAVMSLAMLPVGASAETLRFGHANNSSEIAYDL
                     FQEFADRVTKQTNGEVTIRVFPAEQLGKEVDLVKQVKEGALDLSAPSMPAASTLVPAL
                     EMASAPFLWNNWTEAEAIITSAAFDPVFDELRDKHNIVPLTRIWYWGWRNLTTADREV
                     RKPEDMEGLKVRVPESPVWVEMIRAFGAAPTPIPFGEVYTALQQKTVDAQENPIPTIY
                     GRKFYEVQGVLAMTQHMLQNNMIVINKDSLAKLKPEHQILLFQEAARASSMNTYLQQK
                     RENSMLEDIRASGKTKIIADVDRAAFAEKAKAALPALEERWGKENLDRLLAAIEQARK
                     R"
     gene            complement(9325..10032)
                     /locus_tag="VF226_02195"
     CDS             complement(9325..10032)
                     /locus_tag="VF226_02195"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7072824.1"
                     /translation="MASNARISRRVLADQVHEILTERILDRIYRPEERLNIDRIAREL
                     GVSSSPVREALSRLTAEGLVISSSFIGFAVAPMPDAAWFEELYDYRELVETWAVRIVA
                     DLQRNDVIAALHAAIVRLEEGSYGPAFREYCSAHGADEAFHQIMIEATGNRVVARTYR
                     ELNPHLQHARLYLSHPQDAGLVAAEHRAILDAIEKGDGDAAVAAMRSHLKNSRARLLG
                     GPGEDQSTSNLTGEISL"
     gene            10708..12141
                     /locus_tag="VF226_02200"
     CDS             10708..12141
                     /locus_tag="VF226_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7072825.1"
                     /translation="MSSSPAQANTNPTFVTAENAEWAKRLEEALALLRLVAPIALISL
                     VNMGMSVTDTIMVSALFGTEALAAIAVGSDLYSIVFYLCAGILAGIAPFYTTAVAQAN
                     ALERARIERIGWMTVGLLAVSAVPLIWLAPGWLEEFGIQQTLLTDGQGYTRAMALTLV
                     PMLAVILYRTVLTAAEKPKVFLKVTLAMLPLNAIGNYVFMTGVGPIPSFGPTGAGISS
                     LIVALASLAVLVIVVRRGTPHSVSQISEAKPDWRGLVAVLRVGIPIGIATVAELGIFL
                     GATFYAATLGAADVAAHTLTLRTAGVVYALPAALLQASMVRMARAESLHDAGACRALI
                     FSSLGLSATFGTAIFLTLAIVAEPFATAFFDDSAVGMAAMQLAVVLLILLGLVELLIG
                     PGSAAAGLLRGRKDTRAPMIYVLVGHWAVGAPLGIYLCEAHGLGAIGIWIGLAAGTFL
                     TTLLTLTRLFTRTHAFPAVLQHAGHVG"
     gene            12255..13328
                     /locus_tag="VF226_02205"
     CDS             12255..13328
                     /locus_tag="VF226_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7072826.1"
                     /translation="MSSGKPQRRQVLGYLAAGAAGLAAGSHGRTVSAAAASHGEASGL
                     ELRVVDFHSHFVGPAFTPVAGAAAPPAQRDFFREINRKLASREALLASIEEAGITGRI
                     VNTPLEFIRSADVRVDTSLVQRINDHLAELVRDHPGQLYGLATVDAYSGDDAARELSR
                     AVRDLGLRGVFVESAKGDLLLDAPQARPTLAMAASLGVPVFVHPINDQQLRQRFGRYG
                     RRGSMFSRGTINAAALIALLESGTFEELPGLRVVVTTLAIGGVLLAGAFGDGAQIRRD
                     VPELARRHVYVDTMGLNPVLIRSAVDLLGADHVLAGTDWPIFSEKAVADRLQRALTAC
                     GLDAAEQRMVAGGNALRLLGVEK"
     gene            13526..15337
                     /gene="ggt"
                     /locus_tag="VF226_02210"
     CDS             13526..15337
                     /gene="ggt"
                     /locus_tag="VF226_02210"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7072827.1"
                     /translation="MSFRRLGVALLAAVIAVTTLIGSPAEVAAQRARIAPEPGTGQSE
                     KQLARARSYMVSAANPLAVEAGRRVLQEGGSAADAAIAVQLVLNLVEPQSSGIGGGAF
                     LLHWDATAHELKTYDGRETAPAAARADRFLQDGRPLPFDEAVHSGLSIGVPGLVHMLE
                     QVHQRHGKLPWARLFEPAIELAETGFPVSPRLNLLLTWMGAGNFDPAARRLFFDEKGS
                     PRPAGFVLKNPAFADSLRRIAEAGSRAFYSGPIAEAIVAAAAGAPNHPGDITLADLAS
                     YTSKERPPVCITYRRHRVCGMGPPSSGGITVAQTLKLLEPFDLGRGPEAALNPRAMHL
                     IAEAEKLAYADRNRYIGDSDFVDVPETLLDPQYIEDRRKLIDPDWAMPRPAPGIPPGV
                     RRHAFGVDSTQESVGTSHISIVDSAGNAVAMTTTIESAFGSRVLAAGFLLNNELTDFS
                     FRATDADGRPVANAVAPGKRPRSSMAPTIVFDEAGRLKAVLGSPGGSRIILYVVKALV
                     ALIDWEMDAQSAAALVNFGSRGDAFEIEYDPATDWQALLRPWLSTPSLWHAMRLKPFG
                     HSIAPDLLTSGLHIIVVREDGLEGGADPRREGVAVGD"
     gene            15480..>16001
                     /locus_tag="VF226_02215"
     CDS             15480..>16001
                     /locus_tag="VF226_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7072828.1"
                     /translation="MPSAMEILALFLATIVAAVINALAGGGGLITFTLLLLVVHPVAA
                     DATSAIALVTGYPAAVWRTRDELTQAIRCVWLWLLLIPCVLGGLAGALVLSWAGDRDL
                     LDLVPWLVLGATLLILLRPYLTSHANSGRVEPNLGPWTWPVAMVAMFVIALYGGYFGA
                     GVGIMVIGALGYMG"
BASE COUNT         2941 a         5142 c         4965 g         2953 t
ORIGIN      
        1 gctggccggc gttgtgcggg cccatacttc cgccaaggag gccgggctga ggctgatcgt
       61 cggcgcccgc ctcgacctcc aggatgcgcc gagcctgctc tgcctgccga ctgatcgggc
      121 cgcctacggc cgcctctgcc gactcctgac gctcgggcag cggcgggcgg aaaaagggca
      181 atgcacccta tttctcgacg atgtcgcggc ccaccgcgaa ggccagatct tcatcgcctt
      241 gccaccggaa gactgggatt ggcgcgaggc gatatctcag gaaaaacaaa gccatggcgc
      301 cacggtggtt ccattcccgg cccgcgcaca acagaccccc tctccccggc ggggagaggg
      361 ttggggtgag gggggtccgg cggttgcagt cagcgggatt ggcccaaccc ccaaatcctc
      421 tccacatgag gcgagcgaaa ggcccttcga aaccgagctg cggcgcatca aggcggcact
      481 ggcaccggcc cccgtctatc ttgccgcttc gcatcgctac caaggcgatg accgggcgcg
      541 cattgccgcc ctttcccggc tagctgagcg cgcaggcaca ctgctcgtag ccaccaacga
      601 cgtcctctat catgtgcctg agcgccggcc cctgcaggac gtgctcacct gcatccgcga
      661 gggcacgacc attcgcgagg cagggctcaa gctcgctccc aacgccgagc gccatctcaa
      721 gggaccatcc gaaatggccc ggctctttgc cggctacgaa gcagcagtcg cccgcaccct
      781 cgaaattgcc gcggcctgcc gcttctcgct cgacgagctc gtctatgaat accccgacga
      841 gccggtgccg ccaggcaaga cccctcaatc ccacctcgag gagctgacct gggagggcgc
      901 ccgctggcgc tttcctgaag gcattccgga aaaagtcgcc gctctgatcc ggcgcgagct
      961 cgggttgatc gcccagctca actacgctcc ctacttcctg accgtctacg acatcgtcaa
     1021 gttcgcccgt tcccagaaca tcctggccca gggccgcggc tcggcggcga actccgtcgt
     1081 gtgctactgc ctcggcatca cggcggtgaa tcccaccgag atcgatgttc tgttcgagcg
     1141 cttcgtctcg cccgagcgca aagagccgcc cgacatcgac gtcgatttcg agcacgagcg
     1201 gcgcgaggag gtgatccagt acatctatgc ccgctatggc cgcgagcgcg cagggctcgc
     1261 cgccaccgtc atctcctacc gcgcacgctc ggccgtacgc gaggtcggca aggccatggg
     1321 cttgtcggag gatacggtcg cggcgctggc cggaatggtc tggggcatct cgggcgggac
     1381 agtgccggag tcgcacgtgc gcgaggcggg cctcgatcct cgcgatccgc tgctcgctac
     1441 ggttctcgcc ctcgccgacg agctgatcgg ttttccgcga catctctccc agcacgtcgg
     1501 cggatttgtg cttacccgct cgctgctatc ggaggtggtg ccgatcggca atgcagcgat
     1561 ggaggaccgc accgtcatcg agtgggacaa ggacgacctg gcagctctcg gcctcctcaa
     1621 ggtcgacgtg ctcgccctcg gcatgctgac ctgcatccat cgcgccttcg atctcatcga
     1681 gactcactat ggcgagcact ggacactcgc caacattccg cgcgaggact cgaaggtcta
     1741 cgacatgctc tgccgcgcgg actcaatcgg cgtgttccag gtcgaaagcc gagcgcagat
     1801 gaatatgctg ccgcgcctca agccgcgcac cttctacgat cttgtcatcg aggtggcgat
     1861 cgtccgtcct gggccgatcc agggcgacat ggtgcacccc tatctccggc gccggtcggg
     1921 gatcgagccg gaggtctatc cctcgcccgc gcccgagcac ggaccaccgg atgagctgcg
     1981 ccagatcctc ggcaagacca agggcgtgcc cttgttccag gagcaggcca tgcgcatcgc
     2041 catggtggcg gcgaaattca cggacgcgga ggtcaacgag ctgcgcaagg cgatggccac
     2101 gttccgccgc gccggcacca tcgggaagct cgagcacaag atggtgagcc gcatggtggc
     2161 acgcgggtac gatcctgaat tcgccgcgcg ctgcttcagc cagatcaagg gcttcggcga
     2221 gtacgggttt ccggagagcc acgcggccag cttcgcccac ctggtctacg tctccgcctg
     2281 gatcaaatgc cactacccgg cggtgttcgc cgcggcgctc ctcaactcgc agcccatggg
     2341 cttctacgcc ccggcccaga tcgtccgcga tgcccgcgag catggtgtcg aggtgcgccc
     2401 ggtggacatc aacttctccc actgggactg cacgctggag cccgtcgctg agagaggcga
     2461 aaggcccgta ctcgcgctcc ggcttggcct gcgccaagtc gatggcctga tcgaggatga
     2521 cgcccagaag atcgttgccg ctcgcaccgt gtccgcaatc gccggacccc cctcaccccg
     2581 accctctccc caccggggag agagagtcag gccttatcgc gacgtgcacg atctttgggc
     2641 gcgggccggc gttcgactgg cgaccatcga gaaactcgcc gcggccgatg cgttccgctc
     2701 ggtgggactc gatcggcggc aggcgttgtg ggaggtgcgt gccctgacgg ccgctcgtcc
     2761 gttgccgctg ttcgcctgga gcgaagcgcg ggaagctggc gcggaaccgg cagtcgcctt
     2821 gccggaaatg cagctccctg agcacgtcgt gaacgactac cagaccctgc ggctgtcgct
     2881 caaagcccac cccgtgagtt ttttgcgccc ccaactcgcg ggcgagcggg ccattccctg
     2941 tgccgcgctc cgcaatctca aggacggcca gtgggtgacc gtggcgggcg tggttctcgt
     3001 gcgccaacgg ccaggctccg gccagggcgt cgttttcatg accctggaag acgagacggg
     3061 cattgccaat atcgtcgtct ggccgaaaat gctcgagcgc ttccgcaagg tcatcatggg
     3121 aggacggctg attctcgtgc gcggccgcgt gcagcgccac gaggacatca ttcacgtcgt
     3181 tgcgacctcg ctcgcggacc gaagcgatct gttgcttgag ctgtccgaat ggaaagagga
     3241 tttcaacttg gccctggcca acgcagacga agtcaagcga cctgaccccg gctcggccag
     3301 gaacacgggc cacccccgct tcgcccgcca cccgcgggag gaacgcacca ttccgaaacg
     3361 gctcgaagcc gtcctaccca aatcgcgcga ttttcattga aggaggatgg cgcgagccgc
     3421 attcacggcg ccctcgcttc tcccctggcg ggtaacctcg aggcgatttg gactctgcgc
     3481 cggcgttacg ctgacgaaaa ctaaaggaac ttttcaaact cgaggagaca ttcctcgtgg
     3541 acggcgcatt gccattgcaa gaagccgggc gccatggtca tgtgggcggc agttctaacc
     3601 tgcaagccca gaatgacggg aggagtcctc cgatgcagga caatttgacg gctgcctcgg
     3661 ccgaaccgcg tacacttctt ctcaacgatc gggacaatgt catcgttgcc gtggatggcc
     3721 ttgcacccgg cgcggtcgcg cggggcgtca ccgcaagcag ccgcatccca cgcggccata
     3781 agatggccat cgcaaagatc gccaagggcg agccggtgct gaagttcggg cagattatcg
     3841 gtttcgccac ggaggacatc gcacccggcg agcacgtgca cacgcaaaac tgcggatttg
     3901 ccgccttcga aagggattac gcattttgcc aggacgccaa gcccgagccg ctgctgccgc
     3961 cggaggcccg cgcgacgttt caagggttcc gtcgcagcaa tggccgcgcc ggtacacgca
     4021 attacatcgg catcatcagc agcgtaaact gctcggcgac ggtcgttcgt ttcattgccg
     4081 aggaggtcaa gcgttccggg ctgctcgacg attatccgac tatcgatggc gtcatcccgc
     4141 tggtgcacgg gacgggctgt ggcatcgatt ataatggcga gacgttcgag atcctgaaac
     4201 gcacgacctg gggctacgcc tgcaatccga acatggccgg cgttctcgtt gtcggtctcg
     4261 gctgcgaggg ctttcagatc gcccgcatga aggaagctta tggcatcacc gagagcgata
     4321 catttcggac gatgaccatt caggagaccg gcggcacgaa gaagacgatc gcggccggcg
     4381 tcgatgcaat tcgcagcatg cttccgatcg cggcaaaggc gcggcgcgag acgattcccg
     4441 cgtccgagct gatggtggcg ctgcagtgcg gcggctccga tggctattcc ggcatcaccg
     4501 ccaacgctgc tcttgggctt gcgaccgaca ttctcgtgcg gcacggcggc acgggcgtgc
     4561 tggccgaaac gcccgagatc tacggcgccg agcacctgct gacccgccgc gccgcgactc
     4621 gcgaggtcgg cgagaagctg gtcgagatca ttcagtggtg ggaggactac acggcgcgcg
     4681 cccgcatgag catggacaac aacccttcgc ccgggaacaa ggcgggcggc ctcacgacga
     4741 ttctcgagaa gtcgctcggt gcggcagcca agggcggctc gacgacgctc aacgccgttt
     4801 accggtatgc ggagccgatc acggccaagg gcttcgtctt catggatacg cccggctacg
     4861 atccggtttc ggccacgggc caagtggctg gtggctgcaa tatcgtctgc ttcacgactg
     4921 ggcgcgggtc cgcctatggc tgcaaaccga gcccctcgat caagctcgcc accaactcga
     4981 ccgtctacca ccatatgatc gacgacatgg acatcaactg tggcgacata ctcgatggag
     5041 tcccactgga ggccaaggcg caggagatct tcgaaaccat cctgcgcgtt gcctcaggcg
     5101 aacggtccaa gtcggagctg ctcggctacg gcgatgccga atacgtgccc tggcagatcg
     5161 gagcgacgat gtgatggtcg cggtgaaacg ctctcgctga tgaagccatc ttgaaagcat
     5221 ccgcctgcgt tttgctcagc gagagcgact gtttctagtg tgcaaccgag tacaccggtt
     5281 tttcagcagc gctaggcctc acgccctgaa tacctgggcg aatgtctggt ttttgcggat
     5341 gaattcccaa tcataagtga cgccgagacc gggtccggtc gggaccggta cgcagccgtc
     5401 ctcaccgatc gcgtcgagct gatcggaata gccgcaggtg tagaccggcg gcaccgcgtt
     5461 tggcgtgtcg ggccccacca aggccagctc gtagaagttg gtgttgcgtg tcgccgccat
     5521 gcagtgccgg tgcgccggcc cgcaggcgtg gatctcgacg tcgacgccga acgcctctgc
     5581 cagatgcgcg attttcatcg cgcccgtaat tccgagatcg tactcaggat cggagcgcag
     5641 catgtcggtg ccaccggcga tgaggaaatc cgccttggtc tcgaacccgc gcaggtactc
     5701 ggtctgaaga atgggcgttt tcagcatttc gcgcagcttc ttgtgcgcga acgccgacac
     5761 gccgcagtcc cgcagcgggt cttcgtacca gaaatagccg ccctcatcgc aagcccgccc
     5821 cacgtagagc gcatcagcga atgtccgcag ctcgttagcg gggtcgagca tcagcgtcat
     5881 acgatcaccg acggccttgg caacatgcag cacattgcgc gcttcctccc gcggatcgcc
     5941 gtcgtgccag ccatggatct tgaaggcacg gtagcccatc tcgtagcagc gctcggcgaa
     6001 atcggcgtac gcatctggag aatccagtcc gccattgcgg tcgccgtggt aagtcgaggc
     6061 gtatgtcttg aggcgggttc ggaatccacc aaggagctgc gagaccgacg cgccaaagcg
     6121 cttaccggca aggtcccaga gcgcaatgtc gatcgggccg atgcccatgc catcgtgctg
     6181 gcgcagctcg cgcttcatgt cgttccagat ttcctcccgg gcatctggat cgcggccaat
     6241 gaggttgggc gcgagagcga gcgtctgcgc catcgacgcg cgcgtgccgc cccagtgagt
     6301 cacatattgc ccctcggcgc cgtcatcact gcgaatgacg acggcgtatt tggtcagctc
     6361 gaggttgccg ccgggcttgt atccaatgtt gtagacggca cgcgccgact ctgccgaaag
     6421 cccgagattg gcgaccgtga atttgaactc gatgaccgcg acttcactga tcctggccat
     6481 gtcccaaacc aatccttgtc cggtgtccat tcctgccgtt cagaacccga gaactctggg
     6541 caaccaaagg cttagcccgg gcacgtaggt gataaagagg agcgccaaca gcaccgcgat
     6601 cagcagcggc catagctcct tgatcagcgc cgtcagtttc aacccggtca ttgccatcac
     6661 gacaaacaaa agcacgccca cgggcggggt cagctgcccg agcacgagat tgacgagcag
     6721 aatgatgccg aaatgcacga ggtcgatatc gagactggtc agcaatggca gcaggatcgg
     6781 gacgagaatg atcatgatgg cgagcgtttc catgaccatt ccagcgagca gcagggcgac
     6841 gttcaggatc agcagcacga cccaagggtt cgtggaaatg cttgatacga attcgacgac
     6901 cagctgaggc gcctgctcga tgccgagaat ccagctgaac ggcgtggccg cggccacgat
     6961 gaaaaggatg acggccgtct cgcgggccgt ctgtaccagg atgcgcggca ggtcgcggag
     7021 agacagcgat cggtagatca aaaaacctac gatcagggca taggccgcag cgacgccggc
     7081 aatctcggtc gcggtgaaca aacccatccg gaagccgacc aggatgagca ccggcatcat
     7141 gagtgcccac aaagcacgct tgaactggag ccaaacgttg gccagcgaga actcgccatc
     7201 gggccggaag ccgtgcacgc gcgcaagaat ggcaatcgcc gtcatcatcg atgcggccat
     7261 caccagacct ggcagcacgc ccgcgacgaa cagggctccg agcgagatat tggcctgcca
     7321 agcgtaaacc accatcagga tcgatggtgg gatgattggg ccgagcgtgc cagcggtggc
     7381 agataccgct gcaccgaatg ccttgggata gccagcgttt tggagctgtg ggacgaacac
     7441 gcgcgtcgtg gctgccgtat cggcgaccga cgagccagag attccagcca ggaagacgtt
     7501 gccgacaatc gccgtatggg caagcccgcc gcggatgtgc ccgacgagcg cacgtgtgaa
     7561 gccgaacagc cgctcggaga tgcccgattc gttcatcaga gaaccggcca ggatgaacag
     7621 cggaatggcg agaagcggga acgactgcac acccgcaacg accctttgcg cgacgacagt
     7681 gagcggaatg ccgtcaaccc agatgagcag gatggcagat cccgccatcg cgatggcaat
     7741 gggcgtgcca ataaccaaga gcactgcaaa gacgacaagc agcgtcgtga tcattcgtca
     7801 gtctttcggg atatcatttc gacccaaagg gccgattgcg gtccgtcgtc gagaagtctg
     7861 cgcaaaacgt gaagcatcga gagcgcacac ccgatcgtca gtggcaggat cgaccaggcg
     7921 gcggaaatgc caagcatcgg ggtgaggttt ggaaactcgc gcatcgtgta ttgccagccg
     7981 aaccagacca gcactgcgag caccacaaaa gttgctatcg tcgagagcgt gccgagcacg
     8041 atgcgcagcc agcgcggcgc gaacagcagg attacgtcga tgacgatgtg attgcgctgg
     8101 tggaacgcgt aggccgtacc gatcatgatc gcccagccaa acagcaggac gatgaattcc
     8161 tccgaccacg tgagcggatc gtgcagcgca taacggaaga cgaccgctgc aacgaccaga
     8221 acagtcaaca agcccaacgc tgcggcgagg aagatctcct cgccgcgagc tgcggcatca
     8281 tccaaacgac ggaggaatgc acgcatatga tgcaccggat ctagcgcttg cgcgcctgtt
     8341 cgatggcagc caggagccgg tcaagattct ctttgcccca ccgctcctcc agcgcgggca
     8401 acgcggcttt cgccttctcg gcgaaggccg ccctgtcgac atcggcaatg atcttggtct
     8461 tgcctgatgc gcggatgtcc tcgagcatcg agttctcacg cttctgctgc aaataagtgt
     8521 tcatcgatga agcgcgggcg gcctcttgga acagcaaaat ctgatgctcg ggcttgagct
     8581 tggcgaggct gtccttattg atgacgatca tgttgttctg cagcatgtgc tgtgtcatgg
     8641 ccaacacgcc ttgaacctcg tagaacttgc ggccgtagat ggtcgggatc gggttttcct
     8701 gcgcatcgac cgtcttctgc tgcaaggcgg tgtaaacctc accgaacgga atcggcgttg
     8761 gtgccgcgcc gaaggcccgg atcatctcca cccagacagg gctctctgga actcggactt
     8821 tcaggccttc catatcctcc ggcttgcgca cttcgcggtc ggcggtcgtc agatttcgcc
     8881 agccccaata ccagatgcgc gtcagcggca cgatattgtg cttgtcgcgc aactcgtcga
     8941 aaaccggatc gaaagcggcg ctcgtgatga ttgcttcggc ttcggtccag ttgttccaca
     9001 ggaaaggcgc actcgccatt tcgagtgcag ggaccagagt cgaagcggcc ggcatggacg
     9061 gcgccgatag gtcgagcgct ccctccttga cctgcttgac aagatcgacc tccttgccga
     9121 gctgctcggc cgggaagacc cgtatggtca cctccccgtt cgtctgcttt gttacgcggt
     9181 cggcgaattc ctgaaacaga tcgtaagcta tttctgagga gttattcgcg tgtccgaaac
     9241 gcagagtttc cgcggaggcc cctaccggca gcatcgcaag cgacatcacc gctgccagcg
     9301 ccagagattt tataacaaaa cccttcataa gctgatttct cccgtcaagt tgctcgtgga
     9361 ttgatcctcg cccgggccgc caagcagacg ggcgcgcgag ttttttagat gcgaccgcat
     9421 cgctgcgact gctgcgtcgc cgtcaccttt ctcgatagcg tcgaggatcg cgcggtgctc
     9481 ggcagcaacg aggccagcat cctgcgggtg gctcaggtag agccgtgcat gctgcagatg
     9541 cggattgagc tcgcggtaag tcctggcaac aacccggttg cccgtagctt cgatcatgat
     9601 ctggtgaaat gcctcgtcgg caccgtgggc gctgcaatat tcacgaaagg ccggaccata
     9661 gctgccctcc tcaagcctca cgatggccgc atgcaaagcg gcgatcacgt cgtttcgctg
     9721 caggtcggcg acgatccgta cagcccatgt ctcgaccaat tcgcgataat catagagttc
     9781 ttcgaaccag gcggcatccg gcatcggcgc gacggcaaat ccgatgaagg acgaggatat
     9841 gacgagcccc tcggccgtga ggcggctcaa agcttcgcgg actggcgagg agctgacgcc
     9901 cagctcgcgc gctatcctat cgatgttgag gcgttcttcc ggcctgtaga tccggtcgag
     9961 aatacgctct gtcaggatct cgtggacctg atcggccaga actcgacgcg agatacgagc
    10021 attgctggcc aaagcgatgt ccccccgata tggctgccag gtttcagcgt cgtgatgaga
    10081 tgacagctcg cagccggttc atccggcatt tcctgcacca tgcacgatcg agaatcgatt
    10141 ggcaaccgcc ggcatttccg gcaggcgagt tgatgcgcaa cagcagctcg cggtagtagc
    10201 gttcagcaga cgacggtgag caccagattg cctttttcgc cgatcgccgg ctgtcgggcc
    10261 gacaccgccc gagggggaca tcccgaatta gcgctcgaat gtgtagccaa ttggcgacgg
    10321 aggcgtacgg gagaccaccg gccgcccggc cttggcgact ccgaaatact catatcccgt
    10381 agccgtacag acagatcgcc gagcatagtt gattggcaca aacggcccgt ttctggtccg
    10441 ttcggctctg ccgcctgccc gccgcctctg caatatcgcg ccggcgccca ctcccgggcg
    10501 cagagtcggt catggagcgc acttcaatca tcatagccgt cccggaatta atgcgcgggt
    10561 cacctgcgca ctcggcaccg tttgaggccg ggctctaggg tcgactgaac ccaacccaga
    10621 aaaaaacgca gcaccgccgg accgagggcc tgcccctggg gtgccaccgg tgcttccgtc
    10681 ctcagcgcac gaacaagagt tttcgatatg tctagctccc ctgcacaagc caacacgaat
    10741 cccacctttg ttaccgccga gaacgctgaa tgggccaagc gcctcgaaga ggcgctcgca
    10801 cttctccggc tcgtggcgcc aatcgcgctg atctcgctcg tcaacatggg catgagcgtg
    10861 accgatacca tcatggtctc ggcgctgttc ggcacagagg cgctcgcggc aatagcagtt
    10921 ggaagcgatc tctactcgat cgtcttttac ctgtgtgccg gcatccttgc gggcatcgca
    10981 cccttctata ctacggcggt cgcgcaggcg aatgcgctgg agcgcgcgcg tatcgagcgc
    11041 atcggttgga tgacggtggg cctgctggca gtgtccgccg tcccgctgat ctggcttgcg
    11101 ccgggctggc tggaagaatt cggtatccag caaaccctgc tcacagacgg gcagggttac
    11161 acgcgcgcca tggcgctgac gctcgttcct atgctcgccg tcattctcta tcgcactgtg
    11221 cttacggcgg cggagaagcc gaaggttttt ctgaaggtca cgttggcgat gctgccgctc
    11281 aacgccattg ggaattatgt tttcatgacc ggcgtcggtc ctatcccttc gttcggtccg
    11341 acaggcgccg gcatctcgtc gttgatcgtc gccctggcaa gcctcgccgt tctcgttatc
    11401 gtcgtccgcc gcggcacgcc ccactcggtt tcgcagatat ccgaggcgaa gccggactgg
    11461 cgcggcctgg tcgcggtgct gcgcgtcgga atcccgatcg gcatcgccac ggttgccgag
    11521 ctcggcatct ttctcggcgc aacattctac gctgcgacgt tgggggcggc cgatgtggct
    11581 gcgcatacgt tgacgctgcg caccgccggc gtggtctatg ccctgccggc agcccttctt
    11641 caggcatcga tggtccgtat ggcgcgtgcc gaaagcctac atgatgcagg tgcctgtcgg
    11701 gctctgatct tcagcagcct gggactttca gccaccttcg ggacggccat tttcctaacg
    11761 cttgccatcg tcgctgaacc cttcgccacg gccttcttcg acgacagcgc agtgggcatg
    11821 gcggcgatgc agctcgccgt cgtcttgctg atcctcctcg gcctcgtcga gcttctgatc
    11881 ggtcccggat cggctgcagc aggactgctg cgcgggcgca aggacaccag agcccccatg
    11941 atctacgtgc ttgtcggtca ttgggcggtc ggtgcgccgc tcggcatcta tctctgcgag
    12001 gcgcatggct tgggcgcgat cggcatctgg atcggcctcg ccgccggaac cttcctgacc
    12061 acgctgctga cgctcacccg actgttcact cgcacgcatg cctttcctgc ggtcctccag
    12121 catgccggac atgtcggttg aaggccgcac actggaggcg aagagggggc gaaccctgct
    12181 cgtcagattg ctgacggatt gcctggcgat cggctatgtt tctggcatca aacggttcgg
    12241 caggaggaag cgtattgagt tcgggcaagc cccaacgccg gcaagtcctc ggttaccttg
    12301 ctgcaggtgc tgctgggctg gccgccggct ctcatggacg cactgtcagc gctgcggccg
    12361 caagccacgg ggaggcgagc ggtctcgaat tgcgcgtcgt cgactttcac agccacttcg
    12421 tcggccccgc attcactccg gtggccggcg ctgcggcacc gcccgcgcaa cgagacttct
    12481 ttcgcgaaat caatcgcaag ttggcaagcc gcgaagctct gctggcctcc atcgaggaag
    12541 ccgggatcac gggcaggatc gtcaatacac cgcttgaatt catccgcagt gccgacgtta
    12601 gggtcgacac ctccttggtc cagcgcatca atgatcacct cgccgagctg gtgagagatc
    12661 acccgggcca gctctacgga ctggcaaccg tcgatgcgta cagcggcgat gatgccgccc
    12721 gggaactctc ccgtgccgtc cgcgacctcg gccttcgtgg cgttttcgtc gaaagcgcca
    12781 aaggagatct tcttctcgac gcgccccagg cccgcccgac acttgcgatg gcagcctctc
    12841 tcggcgtgcc ggtcttcgta catccgatca acgatcagca gctccggcag cgcttcggcc
    12901 gctatggacg tcgtggatcg atgttctcgc gcggcaccat caatgcggcc gcgttgattg
    12961 ctctgctgga aagcggcaca ttcgaagagc tgcctgggct tcgcgtcgtc gtcacgacgc
    13021 ttgccatcgg tggggtcctg ctggcgggtg cattcggaga cggggcgcag atcaggcgcg
    13081 acgtaccgga gctggcgcgg cggcatgtct atgtcgacac gatggggctc aatcccgtct
    13141 tgatccgcag tgcggtcgat ctcctcggtg ccgatcacgt tcttgcggga accgactggc
    13201 cgatattttc cgaaaaggcc gttgccgacc gcctgcagcg tgctttgacg gcctgcggcc
    13261 tcgatgcagc cgagcagcgt atggtcgccg gcggcaacgc gctgaggcta cttggtgtag
    13321 agaagtgaca ccagacaaca gcaaacagaa tgtcggacgc gccattctcg acaacccggc
    13381 cagggtgact ttcgggccgg cgtgcttcgt gcgatgcagg aaggcaagtc tccagcgttc
    13441 gcgagaatgc tgtaattact ttcgaaacaa cgccgatcgc aggaaacccg agttgatgac
    13501 gataggacgt gttgcgagcg cgcgaatgag cttccgacgt ctcggcgtcg cccttctggc
    13561 ggcggtgatc gccgtaacca cgctcatcgg cagcccggcg gaggtagcgg cccagcgcgc
    13621 gcgcattgcg cccgagccag gcacggggca gtctgaaaag caactcgccc gtgcgcgctc
    13681 ctacatggtc tcggccgcca acccgcttgc cgtcgaggcg ggcagacgcg tgttgcagga
    13741 aggcggcagc gccgccgatg cggccatcgc cgtgcaactc gtcctcaacc tcgtcgagcc
    13801 gcagtcttcg ggcatcggag gcggcgcctt ccttttgcac tgggatgcaa cagctcatga
    13861 gctcaaaacc tatgacggtc gtgagacggc cccggcagcc gcccgcgctg accgctttct
    13921 gcaggacggg cgtccgctgc ctttcgacga ggccgtgcat tcggggctga gcatcggcgt
    13981 tccggggctc gtgcatatgc tcgagcaagt gcaccagcgg catggaaagc tgccctgggc
    14041 gcgcctgttc gagcccgcga tcgaactcgc cgagacgggc tttccggtct cgccccgcct
    14101 caatctgctg ctgacgtgga tgggggccgg caatttcgac cccgccgccc gtcggctatt
    14161 cttcgacgag aaaggaagcc cccgtcccgc aggcttcgtt ctcaaaaatc ctgcatttgc
    14221 ggacagcctg cgccggatcg ccgaggcagg atcgcgcgcc ttctattcag gcccaatcgc
    14281 cgaggcgatc gtcgccgcgg ccgccggcgc acccaatcac ccgggcgaca tcacgctggc
    14341 cgatctcgca agctatacga gcaaggaacg cccacccgtc tgcattacgt accggcgcca
    14401 tcgcgtgtgc gggatgggac cgccgtcgtc gggcggcatt accgtggcgc aaacgctgaa
    14461 attgctcgag cccttcgacc tcggtcgtgg ccccgaggct gcgctcaacc cgcgggcgat
    14521 gcacctgatc gccgaggcgg agaaactggc ctacgcggat cgcaacagat acatcggcga
    14581 ttccgatttc gttgacgtgc ccgaaacgct gctcgatccg cagtacatcg aggaccgtcg
    14641 caagctcatc gacccggatt gggccatgcc gaggccagct cctggcatcc cgcccggcgt
    14701 tcgccgccac gccttcggcg tcgacagcac gcaagagagc gttggaacaa gccatatctc
    14761 catcgtggac agcgctggca atgcggtggc gatgacgacc accatcgaat cggcattcgg
    14821 ctcacgtgtg ctggcggcag ggtttctgct caacaatgag ctgaccgatt tctccttccg
    14881 tgccacggat gcggacggcc ggcctgtggc caacgccgtt gcgccgggca agcggccacg
    14941 aagctcgatg gcgccgacga tcgtcttcga tgaggcgggc cgcctcaagg ctgtcctggg
    15001 ctcgccgggc gggagccgga tcattctgta cgtcgtgaaa gctctggtcg ccctcatcga
    15061 ctgggagatg gacgcgcagt ccgcagcggc gctcgttaat ttcggcagcc gcggggatgc
    15121 cttcgagatc gagtacgacc ccgcgacgga ctggcaggct ctcctgcggc cctggctgtc
    15181 caccccgtcc ctatggcacg ccatgcgcct caagccgttc ggccactcga ttgcgccgga
    15241 tctgctgacc tccggcctgc atatcatcgt ggtgcgtgag gatggtctgg agggcggcgc
    15301 cgaccctcgg cgagagggcg tggccgtggg cgactgatgc gatctgttcg cctttatcct
    15361 gatctcaccc cattttccgc gcgtgaacgg caagcgtcca aattcgatat taatggttta
    15421 ttaaaacatt caccccgtgg ccccgctctg gagtttaggt gaggacatgt tcagcagaca
    15481 tgccgagcgc catggagatc ctcgcccttt ttctcgccac catcgtcgcg gccgtgatca
    15541 atgcattggc cggcgggggt ggcctgatca cgttcacgct actgctgctc gttgtgcatc
    15601 ctgtggcggc ggacgccacc agtgccatcg cgttggtcac gggttatccg gccgccgtct
    15661 ggcgcacgcg cgacgagctt acccaagcga tacgctgcgt gtggctttgg ctgttgctga
    15721 tcccttgtgt tctcggcggc ctggcgggtg cactggtgct gtcgtgggcg ggcgatcgcg
    15781 atttgctcga tctggtgccg tggctcgtgc tgggagcaac cttgctgatc ctcctgcgcc
    15841 cttacctcac ctcgcacgcc aacagcgggc gcgttgagcc caatctcgga ccatggacgt
    15901 ggccggtcgc catggtggcg atgttcgtca tagcgctcta cggaggatac ttcggggcgg
    15961 gagtcggcat catggttatc ggcgcgctcg gctacatggg a
//
LOCUS       DASVDU010000030         5134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_916500, whole genome shotgun sequence.
ACCESSION   DASVDU010000030 DASVDU010000000
VERSION     DASVDU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5134
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916500"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VF226_02220"
     CDS             complement(<1..917)
                     /locus_tag="VF226_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7072829.1"
                     /translation="MSDIAAEVSLSKMRPPPRATTGPVGWARENLFSSWTNTLLTLIG
                     LYIAYVAISGALNWAFFHATWTGENGSACTRPMAGACWPFVTAKFSQFMYGRYPEAYR
                     WRVDLTYILAVAGLVPLMIPWVRGKLYLTIYMVAIFPVVAFILLYGGLGLEKVPTELW
                     GGLLVTLVVASVGIAGAFPIGILLALGRRSKMPFVRWVSVCFIEFVRGVPLITVLFMA
                     SVMLPLFLPDGWTIDKLLRALIMVALFAAAYLAEVIRGGLQAIPRGQYEAADALGLTY
                     PQKMGLIILPQALKLVIPGIVNTLIGLFK"
     gene            complement(931..2130)
                     /locus_tag="VF226_02225"
     CDS             complement(931..2130)
                     /locus_tag="VF226_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7072830.1"
                     /translation="MAVTTPNRSRVSATEQPSLFYRSEVRQVIYQVLLLVGLAFLIWW
                     VANNVAENLRRQNIASGFDFLGRTSGFDISQSLISYSNVSTYGRAFWVGLLNTLLVSA
                     IGIVIATIIGFVIGIARLSSNWLIARLATVYVETIRNVPLLLQLLFWYFAVLKNLPPP
                     RQSMLLAGGVFLNVRGLYLPVPVADPGFGAVVVSILIGIVASIGLYFWARQRQMRTGQ
                     QFPVLWSSLGLIVGLPLLVYLISGSPLHLEYPVLKGFNFQGGVVVQPEFLALLLGLSI
                     YTASFIAEIVRAGILGVPKGQKEAAMALGLTKGQVLRLVVIPQAARIIIPPLTSQYLN
                     LTKNSSLAVAVGYPDFVSVFTGTVLNQTGQAVEVIALTMLVYLTISLLTSAFMNWFNR
                     HMALVER"
     gene            complement(2396..3394)
                     /locus_tag="VF226_02230"
     CDS             complement(2396..3394)
                     /locus_tag="VF226_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7072831.1"
                     /translation="MVSALAVGLGSAALAQEKTLDAVKQRGVLNCAVNIGLAGFSNPD
                     DKGNWTGLDVDYCKAIAAAVLGDPNKVKYIPTTAKERFTALQSGEADVLIRNTTWSMA
                     RDSSLGLSFAGVNFYDGQGFMVKKSLGVSSAKELNGATVCVQTGTTTELNLADFFRKN
                     KMEYQPVVFEKAEECLQAYEAGRCDAYTTDASGLYAQRLQMRNPDEHIVLPEIISKEP
                     LGPSVRQGDSQWFTVVKWVHFALLNAEELGVTQANVEEMKSSSNPDIRRLVGVEGDFG
                     QAIGLDNDWAVRAIKAVGNYGEIYDRNVGKGSRINIDRGLNALWTEGGLQYAPPIR"
     gene            complement(3531..4310)
                     /locus_tag="VF226_02235"
     CDS             complement(3531..4310)
                     /locus_tag="VF226_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7072832.1"
                     /translation="MAQPSAAATLAVQNSVPAVELIGVNKWFGEFHVLRDINLSVAQG
                     EKIVICGPSGSGKSTLIRCINRLEDHQQGKIIVEGIELTSDLRNIEKIRSEVGMVFQS
                     FNLFPHLTVLDNLCLAPVWVKKMPRAEAERVAMTYLERVKIPEQAHKYPGQLSGGQQQ
                     RVAIARALCMNPRIMLFDEPTSALDPEMIKEVLDVMIELAGSGMTMLCVTHEMGFARS
                     VADRIIFMDRGEIVEQGDPVTFFNDPKSERTRLFLSQILNH"
     gene            complement(4499..>5134)
                     /locus_tag="VF226_02240"
     CDS             complement(4499..>5134)
                     /locus_tag="VF226_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX7072833.1"
                     /translation="ALEYALKRSGPMSMAPSQLGCFTKSDPSRDTPNLQYHIQPLTLP
                     KFGEPLDPFPAFTASVCNLRPTSRGHVRIKSPDPSMHPEIRPNYLATEEDRRVAADSI
                     RLTRHIARQPALARYKAEEFKPGPHIDGDEELARAAGDIATTIFHPVGTARMGTDAVA
                     VVDPRLKVHGLDGLRVVDASIMPTITSGNTAAPTMMIAEKGAAMIAEDWRA"
BASE COUNT         1016 a         1537 c         1610 g          971 t
ORIGIN      
        1 tccttgaaca gcccgatgag cgtgttcacg atcccgggga tcacgagctt gagcgcctgc
       61 ggcaggatga tcagtcccat cttctgcgga taggtcagcc cgagcgcatc ggcggcttca
      121 tactggccgc gcggaatggc ctgcagcccg ccgcgaatga cttctgcgag gtaagccgcc
      181 gcgaaaaggg ccaccatgat gagcgcccgg aggagcttgt cgatggtcca gccgtccggc
      241 aggaacagcg gcagcatcac cgaagccatg aacagcaccg taatcaacgg gacgccgcgc
      301 acgaactcga tgaagcagac gctcacccag cgcacaaaag gcatcttgga tcgccgcccc
      361 agcgccagca aaatgccgat ggggaaggca ccggcgatgc cgacgctcgc gaccaccagc
      421 gtcacgagca agccgcccca cagttctgtc ggcactttct cgagacccag gccgccatac
      481 agtaagatga aagccaccac cgggaagatc gccaccatgt agatggtgag gtagagcttg
      541 ccgcgaaccc acgggatcat cagcgggacg agacccgcga cggcgagaat gtaggtgaga
      601 tcaacgcgcc agcgatacgc ctcggggtag cggccgtaca tgaactggct gaacttggcc
      661 gtcacgaatg gccagcaggc ccccgccatc ggacgcgtgc aggcggaacc gttctctcct
      721 gtccaggtcg cgtggaaaaa cgcccagttc agcgcaccag aaatcgccac ataggcgatg
      781 tagaggccga tcagggtcag gagcgtgttg gtccagctcg aaaacaggtt ctcccgcgcc
      841 cagcccactg ggcccgtcgt ggcgcgtggc ggcgggcgca ttttcgagag ggagacttcg
      901 gcggcgatat ccgacatcgg cttccctccc ctaccgctcg accagggcca tgtggcggtt
      961 gaaccagttc atgaatgccg acgtgagcag gctgatggtc aaatagacca gcatcgtcag
     1021 ggcgatcacc tcgaccgcct gcccggtctg attgaggacc gtgccggtga acacgctgac
     1081 gaaatccgga taaccgacgg cgacggccag cgacgagttc ttggtcaggt tcaggtactg
     1141 gctcgtcagt ggcggaatga tgatgcgggc ggcctgcgga atgacgacca gccgcagcac
     1201 ctgacccttc gtgagaccga gcgccatggc cgcttccttc tggcctttcg gcacaccgag
     1261 aatgcctgca cgaacgatct cggcgatgaa gctcgccgta tagatggata ggcccaggag
     1321 caaggcgagg aactcgggct ggacgacgac cccaccctgg aaattgaagc ctttgagaac
     1381 cgggtactcg aggtgcagcg gtgatccgga gatcaggtag accagcagcg gcaagcctac
     1441 gatcaggcca aggctgctcc acaatacggg aaattgctgc ccggtccgca tctggcgctg
     1501 tcgcgcccag aaatagaggc cgatcgaggc gacgatgcct atgagaatgg aaacgacgac
     1561 ggctccgaac cctggatccg ccacgggaac aggcaggtaa agaccgcgca cgttgagaaa
     1621 cacccccccg gccagcagca tgctctgcct gggcggtggc aggttcttca ggacagcgaa
     1681 ataccagaac aggagctgca ggagcagcgg cacattgcgg atggtctcga cgtaaacggt
     1741 ggcgagccgc gcgatgagcc agttggacga cagccgggcg atgccgatga caaaaccgat
     1801 gattgtcgcg attacgatac cgatcgctga aacaagcagc gtgttgagca gcccgaccca
     1861 gaacgcgcgg ccgtaggtgg agacgttgga ataggaaatc aggctctggg atatatcgaa
     1921 gccggacgtc ctgccgagga aatcgaagcc ggaggcgata ttttgccgtc gcaggttttc
     1981 agcgacgttg ttcgcaaccc accagatgag gaaagcaagg cccaccagca ggaggacctg
     2041 atagatgacc tgcctcacct ccgaacgata gaagaggctc ggttgctcgg tggccgaaac
     2101 cctggatcga tttggggtgg tcaccgccat gctgctgcat ttcccctgtg gctttttcga
     2161 acgcggtgga aacggcggca gcggtcattc ccttgggatt gcaacagttg ccgccggcga
     2221 gaccagctca ccccgccccc ctctcctcca gggggagtga gattggagac gggcttggtt
     2281 tggcttgttt ccatctctgt cgacgatctt tcaggatcag gattgctggc gcacacctcc
     2341 gccggattgg agtgcgccga ccgttgagcc ggccggcgca caagtctcgg gcgcgctagc
     2401 ggatcggcgg cgcgtactgg aggccgccct ccgtccacag cgcgttgagg ccgcgatcga
     2461 tgttgatgcg tgagcctttg ccgacgttgc ggtcgtagat ttcaccgtag ttgccgacgg
     2521 ccttgatcgc tcgcacggcc cagtcgttgt caaggccgat cgcctgcccg aaatcaccct
     2581 cgacgcccac gaggcggcgg atatccggat tgctggagga cttcatctcc tcgacgttcg
     2641 cctgggtgac gcccagctcc tcggcgttga ggagcgcgaa atgaacccat ttcacgacgg
     2701 tgaaccactg gctgtcgccc tgacggacgg agggacccag cggctctttg gagatgatct
     2761 cgggcaggac gatatgctcg tccgggttcc gcatctgcag gcgttgggcg tagagaccgg
     2821 acgcgtcggt cgtgtaggcg tcgcagcggc cggcttcata ggcctgcagg cattcttccg
     2881 ccttttcgaa gacgaccggt tgatactcca tcttgttctt gcggaagaag tcggcgaggt
     2941 tgagctcggt ggtggttccg gtctggacgc acaccgtcgc accgttcagc tccttcgccg
     3001 atgacacgcc gagcgacttc ttcaccatga agccctggcc gtcatagaag ttgacgccgg
     3061 cgaagctcag ccccagggag ctgtcacgcg ccatcgacca agtggtattg cggatcagca
     3121 catcggcctc accagactgc aacgccgtga agcgctcctt ggccgtcgta gggatgtact
     3181 tcaccttgtt cggatcgccg agcacggcag ccgcgatcgc cttgcagtag tcgacgtcca
     3241 ggcccgtcca gttacccttg tcgtcggggt tcgagaagcc ggcgaggccg atattgacgg
     3301 cgcaattgag cacgccccgc tgcttcaccg cgtcgagtgt tttctcctgg gccaaagctg
     3361 cgctgccaag gcctaccgca agcgcggata ccattccgaa cttgatcaga tttttcatcg
     3421 cgcattccct ccaatgagac acagcatctc ggttttcgtt tgacggtccg atttgagtcg
     3481 atttcatttc ctttggcgtt cgcggtgacc tttccgatta atggcttctc ctaatgattg
     3541 aggatctgcg acaggaacag cctggtacgt tcagactttg ggtcgttgaa gaacgtgacc
     3601 ggatctccct gctcgacgat ctctccacgg tccatgaaaa tgatccggtc ggcaaccgag
     3661 cgcgcaaaac ccatttcgtg ggtgacgcaa agcatcgtca tgccggaccc ggcaagctcg
     3721 atcatgacgt cgagcacttc cttgatcatt tcgggatcga gcgctgaggt cggctcgtcg
     3781 aacagcatga tgcgcggatt catgcacaag gcgcgtgcaa tggcgacgcg ctgctgctga
     3841 ccaccggaaa gctgccccgg atacttgtgt gcctgctccg gaatcttcac ccgctcgagg
     3901 tacgtcatgg ccacgcgctc ggcctcggcg cgcggcattt tcttcaccca tacgggggcg
     3961 aggcacagat tgtcgagaac agtgaggtgc ggaaaaagat tgaagctctg gaacaccatt
     4021 ccgacttcgg agcggatctt ctcgatgttc ctgaggtcgc tggtgagttc gatgccctcg
     4081 acgatgatct tgccttgctg gtgatcctcg agacggttga tgcagcggat cagtgtcgat
     4141 ttgccggagc cagaggggcc gcaaataacg attttctcgc cttgggccac tgagagattg
     4201 atgtcacgca aaacatggaa ctcgccgaac catttgttca cgccgataag ctcgacggcg
     4261 ggtacactgt tttgtaccgc aagcgttgct gcggcagacg gctgagccat gatccctccc
     4321 gggactcgaa attcattatg gcggaacggc ccgtcgtaat ccgataggcc atttcccgaa
     4381 cggatttgcg gagtatgccc gacgccttcc aactgcgcaa tcatgcgatg cgcctaaacg
     4441 gcgggcgtca attgccgatg tgttgagcgc tcgcaacttg tgcggcgcaa gattggcgtc
     4501 aagcccgcca atcctcggcg atcatcgcag cccctttttc ggcgatcatc atggtcggcg
     4561 cggcagtatt gccggaggtg atcgtcggca tgatcgaggc gtcgaccaca cgcaggccgt
     4621 cgaggccatg caccttcagg cgcgggtcga cgactgcgac cgcgtcagtg cccattctgg
     4681 cggtgccgac tgggtggaat atcgtcgttg cgatgtcacc tgccgcacgg gccagctcct
     4741 cgtccccgtc gatgtgaggg ccgggcttga actcctcggc cttgtagcgc gccaatgctg
     4801 gttgcctcgc aatgtgccgg gtgagccgga tgctgtcggc cgcgacgcgg cgatcctcct
     4861 ccgtcgcaag gtaattgggc ctgatctccg ggtgcatgga aggatcgggc gatttgatgc
     4921 gaacgtgccc gcggctcgtc ggccgcaggt tgcagacact tgccgtgaag gccgggaacg
     4981 ggtcgagcgg ctcgccgaac ttcggcagcg tgagcggctg gatgtgatac tgcagattgg
     5041 gcgtgtcgcg gctcggatcg gatttggtga agcagccgag ctgggaggga gccatcgaca
     5101 tgggtccgct ccgcttcagt gcgtactcga gcgc
//
LOCUS       DASVDU010000031         7674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_915978, whole genome shotgun sequence.
ACCESSION   DASVDU010000031 DASVDU010000000
VERSION     DASVDU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915978"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VF226_02245"
     CDS             complement(<1..734)
                     /locus_tag="VF226_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX7072834.1"
                     /translation="MAGNLSFEDLKQAVASGEIDTVLVAFTDMQGRLIGKRFHAEFFV
                     DGGAEETHACDYLLTADIDMEPVPGYAAASWQKGYGDFVMKPDLSTLRRIPWLEGTAL
                     VLCDVLDHHHHELPHSPRQILKRQIARLDERNMRAYFASELEFYLFDETFESALAKRY
                     QDLKTAGSYIEDYHILQTTKEEGVMRAIRKGLHAAGIPVENSKGEWGPGQEEINVRYA
                     DALEMADRHVVLKNGIKEIAHLQGKAV"
     gene            complement(927..2321)
                     /locus_tag="VF226_02250"
     CDS             complement(927..2321)
                     /locus_tag="VF226_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7072835.1"
                     /translation="MSMVPERHGGVAQSESDTNLTSRRAEWQRRALDDRTRVLLAEDE
                     RYFLRQSVSTPCLNAIAKAEGIWIEDVTGRRYMDFHGNNVHHIGYGHPRLKRAIAEQM
                     EKLSFAPRRYACEEAVALAAKLAEITPGPLSKVLFTTGGSDANEVAIKLARTATGRFK
                     TLSFWDSFHGAGFGAAALSGEALFRSGPAAPLVAGASHVAPFACYRCPYGYPVDERGQ
                     PRIELCQIACARMVDYVLSREGDVAAVIAEPSRVVPYLPPPGFWKIVREACDRHGALL
                     IFDEIPAGLGKTGRMFACEHDGVVPDILVVGKALGGGILPIAAVIARPELDVGGDWAF
                     GHYTHEKNPVTSRAALTTIEIIQEENLVENARRVGEIALERLKEMKRKLPAIGDVRGR
                     GLLLGIELVKDRASKEPDNDLAEAVMYAALDGGLSFKTTMGNVLTLTPPLTVTADQML
                     RALDILEEAIRANA"
     gene            complement(2330..4024)
                     /locus_tag="VF226_02255"
     CDS             complement(2330..4024)
                     /locus_tag="VF226_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEX7072836.1"
                     /translation="MTMAAVAAPAAARQLSREDWVMRIGITILCVLLVIAIALPLGLL
                     LFKSFQRDGHFVGLANYAVYFSTPTLVASIWNSLFVAISATVITVPLAFAYAYALTRT
                     CLPLKGVFMAVAMLPIFAPSLLSAISFIYIFGNQGFLKELLLGASVYGPIGILMALVY
                     YCFPFAVLILVTALSLADGRLYEAAAALGTSAWRVFWTVTVPGIKYGLVSASFLVFTL
                     SITDFGIAKVIGGQYNVLATDVYKQVVGQQNFEMGAVVGFVLLVPAVVAFAVDRLIQR
                     KQIALLSARAVPLEPKPVRRRDVAFLVYCCLIGALIIGILAVAVWASFVTYWPYNLSL
                     SLKNYNFDEFDPHGWETYFNSLEMASLAAILGTAVVFVGAYLVEKGQGFRAGRALAHF
                     LAMLPMAVPGLVLGLAYVFFVNAPWNPLNVFYGTILVLAVNTIAHFYTAAHITAVTAL
                     KQIDSEFESVSASLKVPFWRTFIRVTVPICMPAILNIAVYMFVNAMTTVSAVIFLYGP
                     STKLASVSIVHMDEAGFTAGAAGMATLIVLTALGVKLLHFALDWLVFGKLQAWRKR"
     gene            complement(4021..5163)
                     /locus_tag="VF226_02260"
     CDS             complement(4021..5163)
                     /locus_tag="VF226_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7072837.1"
                     /translation="MLQTLERPSSTPAGDTNLRPVHLRISNLTKRYGAFTALKSISLE
                     VYAGELICFLGPSGCGKTTLLRAVAGLDMQNEGRIEMGGRDVSHLPPSARDFGIVFQS
                     YALFPNLTAAANVGYGLVNRRMPRAKVEARVKELLELVGLPDQGSKYPAQLSGGQQQR
                     VALARALATSPSLLLLDEPLSALDARVRLRLRDEIKALQRRLGVTTILVTHDQEEALA
                     MADRIVVMNNGVIEQVGTPAEVYGAPATAFVADFIGHMNFLDAEIAGPDRARVGNVLL
                     RSTNVGGRQSGTRVKLGLRPEEIRVRGVGPDTENTVTARVMLLDYLGAFCRATLDIEA
                     APGVQLLADFSANAIRDLGICEGQALTAALPPESLRVFSHEEVVSE"
     gene            complement(5238..6266)
                     /locus_tag="VF226_02265"
     CDS             complement(5238..6266)
                     /locus_tag="VF226_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7072838.1"
                     /translation="MFKHFVRVGVAACAAVLMAGLPAQARTQLTIYTALENDQLAPIK
                     AAIEKAVPEVEVVWVRDSTGVITARFLAEKDNPRADMVLGIAATSLLMFENEGLLEPY
                     KPKGVEQLKEVFRDAKEPYTWTGMDAYLGVICFNTAEAAKSNVPVPTSWKDLTKPEYK
                     GKLAMPHPASSGTGFLMVGAWLQTMGEEEGWKFMDALHENMAVYTHSGSAPCVQAAKG
                     ERLAGIALDMRGAKEKTQGAPLEVIIPAEGTGWEMEATGIVKGTKHLELAKKVADWGA
                     TKEANQIYGKTYAVVAYPGAASYPPNYPAHAEERMIKNDLSWMAENRDRILAEWTKRY
                     ESKAAPKN"
     gene            complement(6664..>7674)
                     /locus_tag="VF226_02270"
     CDS             complement(6664..>7674)
                     /locus_tag="VF226_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7072839.1"
                     /translation="GCISAITPFNHPLNMISHKIAPAIATNNRVVAKPTEQTPLTALL
                     LADILYEAGLPPEMLSVVTGNPRTMGDAMITDPRADIVTFTGSVKVGKYIAEKAGYKR
                     LILELGGNDPLIVMEDADLDKAAELAVQGATKNSGQRCTAVKRILVVESVADQFAELV
                     LAKARKLKAGDPMDPATDIGTVIHEGAAKLFEARVNAAVEAGAKLLLGNDRKGALYPA
                     TIVDHVPYDCELVKEETFGPVIPIIRCPDDIGEVIRISNSTAFGLSSGVCTNRLDYIT
                     RFINELHVGTVNIWEVPGYRIEMSPFGGIKDSGLGYKEGVWEAMKSYTNIKTYSLPWP
                     QG"
BASE COUNT         1462 a         2454 c         2369 g         1389 t
ORIGIN      
        1 acggccttgc cctgcagatg cgcgatctcc tttatgccgt tcttcaaaac gacgtgccgg
       61 tcggccatct ccagcgcatc ggcatagcgg acgttgatct cctcctggcc ggggccccac
      121 tcgcccttcg agttctcgac cggaatgccc gcggcgtgca gacccttgcg gatggcgcgc
      181 atcacgccct cctctttggt ggtctgcagg atgtgatagt cctcgatgta ggagcccgcg
      241 gtcttcagat cctgataccg cttggccagt gcggactcga acgtctcgtc aaaaaggtag
      301 aactcgagct ccgacgcgaa gtaggcccgc atgttccgct cgtcgagacg ggcgatctga
      361 cgtttcagga tctggcgagg cgaatgtggc agctcatggt ggtgatgatc gagcacgtca
      421 catagcacga gcgcggtgcc ctcgagccac ggaatgcgcc gcagcgtcga caggtcgggt
      481 ttcatgacga agtcaccgta ccccttctgc caactcgcgg ccgcgtatcc gggcaccggc
      541 tccatatcga tatcagcggt cagcagataa tcgcaggcat gggtctcttc cgcgccgccg
      601 tcgacgaaaa actccgcgtg aaaccgcttg ccgatgaggc gcccctgcat gtcggtgaag
      661 gcgacgagca cggtatcgat ctcgccggat gctacggcct gcttcaggtc ctcgaaactc
      721 agattgcccg ccatccacgt ctcccacttg accgaaaatt cttgtctcct gtgcacatat
      781 tactgctcga ggcctcgagc agaaaagagg ccagcgtagg cgcatgctct gtcgtctgct
      841 cctcggcccc gcagcgcacg tttctcaact ggatcgagcg ggggcggcga gccaatccga
      901 gctcgggcaa aaagggtccg acgccctcat gcgttggcac ggatcgcttc ctcgagaatg
      961 tcgagcgccc gaagcatctg gtcggcggtt accgtcaatg gcggcgtgag ggtcagaaca
     1021 ttgcccattg tcgtcttgaa ggacagcccg ccatccagcg cggcgtacat gaccgcctcc
     1081 gcgagatcat tgtccggctc cttcgaggcg cgatccttca ccagctcgat gccgaggagc
     1141 agaccgcggc cgcgcacatc gccgatggcg ggcagcttgc gcttcatttc cttgaggcgt
     1201 tcgagggcga tttcgccgac ccggcgcgcg ttctcgacca gattttcctc ctggatgatc
     1261 tcgatcgtgg tgagagcggc tcggctcgtt accgggttct tctcatgggt gtagtggcca
     1321 aacgcccagt caccgccgac gtcgagctcc ggccgcgcga tcaccgccgc aatgggcagg
     1381 atgcccccgc ccaatgcttt tccgaccacc agaatatcgg gcaccacgcc gtcgtgctcg
     1441 caggcgaaca tgcggccggt cttgccgagg ccagcaggga tttcgtcgaa gatgaggaga
     1501 gcgccgtgcc ggtcgcaggc ttcacggacg atcttccaga acccgggcgg cggcaggtag
     1561 ggcaccacac gcgagggctc cgcgatgacg gccgcaacgt cgccttctcg ggaaaggacg
     1621 taatcgacca tccgtgcgca cgcgatctgg cacagctcga tccgcggctg gccgcgctcg
     1681 tcgaccggat agccataggg gcagcgatag caggcgaaag gcgcaacgtg ggatgcccca
     1741 gccaccagtg gagccgctgg accggagcgg aacagggcct cgccggaaag cgcggccgcc
     1801 ccgaaacccg caccgtggaa gctgtcccag aaggacagtg tcttgaaccg gccggtcgcc
     1861 gtgcgggcga gcttgatggc cacctcgttg gcgtccgagc caccggtcgt gaacagcacc
     1921 ttcgacagcg gccccggcgt gatctcggcg agcttcgcgg cgagcgctac ggcctcctcg
     1981 cacgcgtacc gtcgcggtgc gaaggacagc ttctccatct gctccgcaat ggcgcgtttc
     2041 aggcgcggat gcccatagcc gatgtgatgg acgttgttgc cgtggaaatc catgtagcga
     2101 cggccggtga catcctcgat ccagatgccc tccgccttgg caatcgcatt gaggcacggc
     2161 gtcgagacgg actggcgcaa gaaatagcgc tcgtcttccg ccagcagcac gcgggtgcgg
     2221 tcgtcgaggg cgcggcgttg ccattcagcg cggcggctgg tcaggttcgt atcgctctcg
     2281 gactgggcga caccgccgtg ccgctcgggc accatgctca tgggccgcct caccgcttgc
     2341 gccaagcctg cagcttcccg aatacgagcc agtcaagcgc aaagtgcaga agcttcacgc
     2401 caagtgccgt caacacgatg agcgtggcca ttccggctgc acctgccgtg aagcccgctt
     2461 cgtccatgtg cacgatcgac acggaagcaa gcttcgtgga tggcccgtat aggaagatca
     2521 ccgctgaaac ggtcgtcatc gcgttgacga acatatagac ggcgatgttg aggattgccg
     2581 gcatgcagat gggcaccgtc acgcggatga acgtgcgcca gaaaggcacc ttcagcgatg
     2641 cggatacaga ctcgaactcg ctgtcgatct gtttcagggc cgtcacggca gtgatgtgcg
     2701 ctgccgtata gaaatgcgcg atcgtgttca ccgccagaac caggatcgtg ccgtagaata
     2761 cattgagagg gttccacggc gcgttgacga agaacacgta ggcgaggccc agcaccagcc
     2821 ccggcacggc catcggcagc atggccagga aatgcgccag cgcccgacca gcccggaacc
     2881 cctgcccctt ttcgacgaga tacgcaccaa caaagacgac cgccgtcccg agaatggcag
     2941 ccagcgaggc catttccagt gagttgaagt aggtctccca gccgtgcggg tcgaactcgt
     3001 cgaagttgta gttcttcagg ctcagcgaga ggttgtacgg ccagtacgtg acgaacgagg
     3061 cccaaactgc aacggcgaga attccaatga tgagcgcgcc aatcaggcag cagtagacga
     3121 ggaaggcaac gtcccgccgg cggaccggct tcggctccag cggcacggcg cgggccgata
     3181 gcaacgcgat ctgcttgcgt tggatcaggc ggtcgacggc gaaggccacc actgcgggca
     3241 ccagcaggac gaagccgacc acggcgccca tctcgaagtt ctgctggccc acgacctgct
     3301 tgtagacgtc cgtcgccagg acattgtact ggccaccgat gaccttcgcg atgccgaagt
     3361 ctgtgataga cagcgtgaag acgaggaagc tggcactgac gaggccgtac ttgatccccg
     3421 gcaccgtgac cgtccagaag acgcgccagg ctgacgtgcc gagcgccgcc gcagcctcat
     3481 agagccgccc gtcggccagc gacagcgcgg tcacgagaat gagcacggcg aatgggaagc
     3541 agtagtagac gagcgccatc aggatgccga tcggcccata gaccgaggcg cccaacaaca
     3601 gctccttcag gaagccctgg tttccgaaga tgtagatgaa cgagatggcc gagagcagcg
     3661 aaggcgcaaa aatcggcagc atcgcaaccg ccatgaagac gcccttcagc ggcagacagg
     3721 tccgcgtcag cgcataggca taggcgaagg cgagcggcac ggtgataacc gtcgccgaaa
     3781 tagcaacgaa caggctgttc cagatggagg cgacgagcgt cggagtcgag aaatagacgg
     3841 catagttggc cagcccgacg aagtggccgt cgcgctggaa gctcttgaaa agaaggagcc
     3901 ccagcggcag ggcgatagcg atgacgagca gcacgcacag gatcgtgatg ccgatccgca
     3961 tcacccagtc ttcacgcgag agctgacgtg cggcagcggg cgccgctacg gcggccatgg
     4021 tcattcggaa acgacctctt cgtgcgaaaa gacacgcagg ctttcgggcg gcagcgccgc
     4081 ggtcagcgcc tgaccctcgc agatgccgag atcacggatc gcgttggccg agaagtccgc
     4141 caagagctga actccgggag ccgcctcgat gtccaatgtc gcacggcaga aggcgccgag
     4201 gtaatcgagc agcatcacgc gtgccgtcac agtattctcc gtgtcggggc ctacgccacg
     4261 cacacggatt tcttccggcc gcaagccgag cttcacgcgc gtgccgctct ggcggccacc
     4321 gacattggta gagcgcagca agacattgcc gactcgggct cggtcgggcc cggcgatctc
     4381 cgcgtcgaga aaattcatat gtccgatgaa gtcggcaacg aaggccgtag ccggcgcccc
     4441 atagacctcc gctggcgtgc caacctgctc gatgactcca ttgttcatga ccacaatccg
     4501 gtcggccatc gcgagggcct cttcctggtc gtgggtcacc aggatcgtcg tcacgccgag
     4561 gcgccgctgc agggccttga tctcgtccct gagccgcagg cggacacggg cgtccagcgc
     4621 ggagagcggc tcgtcgagca gcagcagcga cggcgaggtt gccagcgcgc gggccagcgc
     4681 gacgcgctgt tgctgtccgc ccgagagctg ggccggatac ttcgatccct gatcgggcag
     4741 accaaccagc tccagcagct ccttcacgcg ggcttccacc ttggcgcgcg gcatccgccg
     4801 attgaccaga ccgtagccga cgttggccgc ggcagtcagg ttgggaaaca aggcgtagga
     4861 ctgaaagacg atgccgaagt cgcgcgctga cggcggcagg tgggagacgt cccgcccgcc
     4921 catctcgatg cggccctcgt tctgcatgtc gagcccggcc acggcgcgca aaagcgtagt
     4981 cttgccgcaa ccggatgggc cgagaaaaca gatcaactct ccggcgtaaa cctcgagcga
     5041 aatggacttc agcgcggtga acgcgccgta cctcttggtg agattgctaa ttctcagatg
     5101 aaccggacgc aaattggtgt cccctgccgg agtagaggaa ggacgttcaa gcgtctgcag
     5161 catagtgcag tcccattgcg ttctcgaaag ggaaatacgg gaggccacat gaaaagtggc
     5221 cgcccgttgt ttctgcatca gttcttgggc gccgccttgc tttcgtagcg cttcgtccac
     5281 tcggccagga tacggtcccg attctcagcc atccaggaaa gatcgttctt gatcatgcgc
     5341 tcttccgcat gggcggggta gttcggcgga tagctggccg cgcctggata ggcaacgacc
     5401 gcataggtct tcccgtaaat ctggttggcc tccttcgtgg caccccagtc ggcgaccttc
     5461 ttcgccagct cgaggtgctt ggtgcccttg acgatgcccg tagcttccat ttcccagccg
     5521 gtgccttcgg ccggaatgat gacctcgagc ggcgctccct gggtcttctc cttggcgccg
     5581 cgcatgtcga gcgcaatgcc cgcgagccgc tctcccttgg cagcctgaac gcacggcgcg
     5641 ctgcccgagt gagtgtagac ggccatgttc tcatggagcg catccatgaa cttccagccc
     5701 tcttcctcgc ccatggtctg cagccatgcg ccgaccatca ggaagcccgt cccggaggag
     5761 gcggggtgcg gcatggccag cttgcccttg tactcaggct tggtgaggtc tttccacgaa
     5821 gtcggaacgg gcacgttgct cttcgccgcc tcggcggtgt tgaaacagat cacgccgagg
     5881 taggcgtcca tgccagtcca cgtatagggc tccttcgcgt cgcgaaagac ctccttgagt
     5941 tgctcgacgc ctttgggttt gtatggttcg agcaatccct cgttctcgaa catcagcaac
     6001 gacgtggcgg caatccccag aaccatgtcg gcgcgaggat tgtccttctc cgccaggaac
     6061 cgcgcagtga tgaccccggt cgagtcgcgg acccagacca cctcgacttc aggaacggcc
     6121 ttttcgatcg ccgccttgat cggcgcgagc tgatcgttct ccagcgccgt atagatcgtg
     6181 agctgcgtac gtgcctgagc cggcagaccg gccatcagaa cggccgcgca ggcggcaacg
     6241 cccactctta cgaaatgctt gaacatgctg tttcactccc tccatttgga cgcacattgg
     6301 ctcgaccagg cccgccccgg ccgactgccg caggcagaca tgctcgctac tcatgaatct
     6361 ctgatgacac caatctccga tgacgccggg attcgcaatg atcgctttca gcccgtgttg
     6421 ttcagattgc atcttagccc aatgccgaag ttgtgaaaac caacatagaa aatcgcatcg
     6481 acctgctctt ggctagcgaa tttatcaggc gactcgctgg atatatgagc aggatcccct
     6541 tccttttctt ggggatattt tccttgggga taggtgagag acggccgtcc gccctcatcg
     6601 aacgcgccgc ctctcaccgg gtcgccccca cccgatcttg aatctcccgc tgccgtcgtc
     6661 tggtcagccc tgcggccagg gcagtgaata ggtcttgata ttcgtgtagc tcttcatagc
     6721 ctcccacacg ccttccttgt agccgaggcc tgagtccttg atgccgccga atggggacat
     6781 ctcgatacgg tagcctggca cctcccagat gttgaccgtg ccgacatgga gctcgttgat
     6841 gaagcgcgtg atgtagtcga gccggttcgt gcacacgccg gacgagaggc caaaagcggt
     6901 cgagttggag atcctgatga cctccccgat gtcatccggg caccgaatga tgggaatgac
     6961 cggtccgaac gtctcttcct tgaccaactc gcagtcgtaa ggcacgtgat cgacaatggt
     7021 tgccgggtag agcgccccct tgcgatcgtt gcccaaaagc agcttcgcac cagcctccac
     7081 agcggcgttc accctggcct cgaacagctt ggccgcgccc tcatggatca cggtgccgat
     7141 gtccgtcgcc ggatccatcg ggtcgccggc cttgagcttc ctcgccttcg ccagaaccag
     7201 ctccgcaaac tggtcggcaa ccgattcgac cacgagaatt cgcttcacgg ccgtgcagcg
     7261 ctggccggag ttctttgtgg cgccctggac ggcaagctcc gccgccttgt cgaggtcggc
     7321 gtcctccatg acgatcagcg gatcgttgcc gccgagctcg agaatcagcc gcttgtagcc
     7381 cgccttctcg gcgatgtact tgccaacctt cacgctgccg gtgaacgtga cgatgtcggc
     7441 ccgcggatcg gtgatcatcg cgtcgcccat ggtccgcgga ttgcccgtca cgacggacag
     7501 catctccggc ggcaggcccg cctcgtagag gatatcggcg agaagcagcg ccgtcagcgg
     7561 cgtctgctcg gtcggcttcg cgaccacgcg attgttggtg gcgatggccg gcgcgatctt
     7621 gtggctgatc atgttcagcg gatggttgaa cggcgtgatc gcggagatgc agcc
//
LOCUS       DASVDU010000032        27102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1500057, whole genome shotgun sequence.
ACCESSION   DASVDU010000032 DASVDU010000000
VERSION     DASVDU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 27102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27102
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500057"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VF226_02275"
     CDS             <1..997
                     /locus_tag="VF226_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7072840.1"
                     /translation="LANGATTLMFEGVPNYPTPSRFWHVVDKWGVNIFYTAPTAIRAL
                     MGAGDDFVKRTDRSSLRLLGSVGEPINPEAWEWYYRVVGESRCPVVDTWWQTENGGIL
                     ISPLPGAMKLKPGSATLPYFGVVPGIVNDKGELIEGPGSGNLVILDSWPGQMRTVYGD
                     HERFVQTYFSTYPGMYFSGDGCRRDEDGYYWITGRVDDVINVSGHRMGTAEIESALVA
                     HPKVAEAAVVGYPHDIKGQGIYCYVTLLAGETPSDELKKELVDHVRKEIGPIAAPDLI
                     QFAPGLPKTRSGKIMRRILRKIAEDDFSNLGDTSTLADPSVVDDLINNRQNRRAG"
     gene            complement(1074..1784)
                     /locus_tag="VF226_02280"
     CDS             complement(1074..1784)
                     /locus_tag="VF226_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX7072841.1"
                     /translation="MRKRRLKIGRYLLLLMLLAGIGLAFRQGLVPARYSPLPALDLER
                     PLPLIVDWQLAELRYDAKLCSRVLVPPHIESVPIRDNPLNKGCGWINSVRVSSVGGAR
                     LPVDKLSCPAAAALSLWMVHQVQPLAQSMFGQRVKSLEHMGTYSCRNIVGNAFWKNKR
                     SEHATANAIDISAFTLEDGKRISVLKDWRGQTPEAEFLRLVHAGACNYFRVALSPEFN
                     RAHRDHFHLDRGILSTCK"
     gene            complement(2267..2491)
                     /locus_tag="VF226_02285"
     CDS             complement(2267..2491)
                     /locus_tag="VF226_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072842.1"
                     /translation="MGTIFFRCPVTGQEYASNIEIDEASFSRLPDIKMKTRCPHCGAD
                     HVWSLREARLSPQKLAGVPPATEQDGQTSN"
     gene            2731..3033
                     /locus_tag="VF226_02290"
     CDS             2731..3033
                     /locus_tag="VF226_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072843.1"
                     /translation="MTMMSRTSAGRLATVSTIALVNIVGIAAISSSAKAVSLRVQMAC
                     ASDYFSYCSKYPTEGPQVRRCMRANGHKLSPACVNALVAAGEVSKAEVARRSAGAN"
     gene            3203..4381
                     /locus_tag="VF226_02295"
     CDS             3203..4381
                     /locus_tag="VF226_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEX7072844.1"
                     /translation="MTDATATTPMQYLDRATRHLRDIGLMPAKVEPAPINALLEKISD
                     LDEERITLIARTLGQAEVFNEVVRQETAGMAIGQRYQEITDGFNSIRDDARRLVDQVS
                     DGKLNIFERGSNVWMKIVRGDIADRFEKIRQVYLAVTAETKNQIEREAKILDAYSDFR
                     GALKHAEVMALEVLKKAEDKLEAARKRLDEASAKVAGFTGTEPAERAKLELARDEQLR
                     ALQEEEKRYQIAKDLSDNLTIAYNTSEVIMGRLMQTTSAKERVYAQAVSFFSTNDSVL
                     TALKASFTGVFGLHESTKTLEAMKEGMSKSLEDLAKLGDKVQEEALRAGYGPTIRADA
                     VKQLIDSVINFQERSREIINEMRKLATQNSAEIREAVEDGKRRIARLAAEAQLLPARH
                     "
     gene            4404..5348
                     /locus_tag="VF226_02300"
     CDS             4404..5348
                     /locus_tag="VF226_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6384 family protein"
                     /protein_id="HEX7072845.1"
                     /translation="MVSDTAVEPKPHTLDELMIAMDVVDTLRHREDLIRRELDEAGRE
                     AELIARLKEIYQQQGIDVPDRVLEQGVKALKESRFVYTPPPPSWKRTILEAWARRGVI
                     GKRVLATLAVLLILAGVVYFGLVRPVQQARQQALIEATETLPRAIRTAHSDIAQIATD
                     EAARSRADALLADGERAIRDRDVAGMRRANAELAQLLDTVSREYTLRIVSEPGKLTGL
                     WLSPPGWFKERRYYLIVEPVTPDGQKLTLPIRDEVTGETKNVSQFGVRVPRKTYEAVA
                     EDKRPDGIVDNNLFGVKLRGRLQVDYRMPFEGGTINTW"
     gene            5365..6882
                     /locus_tag="VF226_02305"
     CDS             5365..6882
                     /locus_tag="VF226_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072846.1"
                     /translation="MWTGRQAFERIENAIVGLHRQETELDSALASAAADAERLRRERS
                     DAFRELARIKLDEITAQRLVDDLDAAETRAVQLLEGRRRQLESITAQREQAIIALERA
                     EAERAEAAAALERAIDALDELRSKVADEIRSSEEWNRARAALEKATKVADAADAKAAL
                     SEAELSEKRQPYDNDPLFAYLWHIGYGTPRYRAGSFARFMDRMTARFIGFPDARLNYA
                     MLTEIPARLREHARLQREIADERRAELLAIEQKAIAAAGEGTLSREVEEARRKLAAAD
                     EAIESRQSELRNLDQKRDALVAGVDDPAYREALQTIASADAQDQFENLVAEAKRTATE
                     ADDAIVRRIGDLNERIDRIEAEIAELRKSARAIAERRVEVERARDRFRESGYDHPDAT
                     FDNDVDLDRILRQVLGGVVTGGVLWDLLRSGFGLRYPGGRRSGGLSFPFPFPIPMPDR
                     REDTRGGDWRRADSRGGWSPTHESWKRSRWDRDDDDDDDDHDHGHRGGWRTGGRF"
     gene            7173..8498
                     /locus_tag="VF226_02310"
     CDS             7173..8498
                     /locus_tag="VF226_02310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7072847.1"
                     /translation="MEATRRSALQLILASGLLSCFPRIAVAGPAVCRTGILTSHRLGN
                     GLRVHCYRNGSKYVSAALVLRSCEILNHSGLAHILEHTSFTGAAGNYSAKELKRRHRT
                     FIQDSNASTSPGRLEWYASFLPKYAPEAIELLAITSLDQKCDLRTVESEAKIVLQELL
                     LDKHSGDAAIRRQFNAALYGENHPYAVDNCDAEIAKVKLPARELARELTEYASLIRQP
                     ANMDLVLVGDVETGDISQLVEKCFGCYPHAQGPVLEIPKAQPTREYRSIEGVSGDLER
                     PLSEIRIGWNTGVTINDPDARVLLTLGAYLNDVIFSEVRERHGDSYSPEAYFEPDGCS
                     GIFTISAITSKHPELVEERIFSAIERVKEEIDPLELRLHKERWELKRLRTAESGSSML
                     ECLVARIVEGCAVEDLDIESVSPAEMVEAARKYLPDYKGAYTRVTLNGA"
     gene            complement(8505..10130)
                     /locus_tag="VF226_02315"
     CDS             complement(8505..10130)
                     /locus_tag="VF226_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7072848.1"
                     /translation="MGASGTPIEGVVAADEAGNVLFASGIGNDSRVKALVSDQAWRAE
                     IRERRLQPLILDRNSYAILDVPMSGGDLLVISRPKSNALLEFIGSVEFAYDILEHMLT
                     DPFEAMTIVDADARLVYISPVHESFFGLQRGEANGKPVKEVIENTRLDQVVATGKAEV
                     GEIQRMRGLDRVVSRIPIRRDGRILGAVGRVMFKGPQQVEELGRRIHALESEVEFYKR
                     ETAALRSKSYGLDQLIGDSPAMRRLRDEIVKVAPLEIPVLIRGESGTGKELVAQALHR
                     LSPRRDAAIVMVNAAALPPTLVESELFGYEPGAFTGAARKGRKGKFEQAAGGTIFLDE
                     IGDMPIEVQSKLLRVLQDRMIERIGSDRPQQVDFRLVTATNRDLQQFVSEGKFRLDLY
                     YRISPITIEVPPLKRRLDDIPALVAHFVADFSVRHRRPAPEITESALSWLMEQSWPGN
                     VRQLRHAVERALIFADDNIITAESFAPQSNGFSRPMPEASAATTLRERLSRIETDLVL
                     KALRRHNGNKKRVAQELGISRSYLYKILGEMKT"
     gene            10442..11752
                     /locus_tag="VF226_02320"
     CDS             10442..11752
                     /locus_tag="VF226_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072849.1"
                     /translation="MGLRFGIGRLSAIGAGAALSVALTASAYAQEPVKLGIVSFLTGP
                     AAVPFGVPGRNAAELIIDAINKGSLPAPYNSKGLGGASIEARYVDEAGSTANQVTEYR
                     NLVQRDGVDAVVGYVSSGNCLAVAPVAEEMKTITVLYDCGTPRIFEEDPNHKYVFRVT
                     PTATMDSVGAARYVVDKKGDIKSFSGLNQNYAWGQDSWRDFVLAMKALAPNATVDREL
                     FPKFLAGEYGAEITSLLTSEAELVHSSFYGGDLESFVAQATGRGLSQRKTMIFSCGES
                     AMFRLGNRMPDGTIIGGRGPHGVLARESELNTWFQKEYAARFGTPPTYPSYHMAQSIL
                     GLKAAWDKAAAAKNGARPSTDEIIAAFEGLEFDGPSTRISMARANGHQGISETAYGVF
                     KFNKEANRPEVVDIVRYPAECVNPPDGVTSQEWLEGGMKGAKCD"
     gene            11845..12774
                     /locus_tag="VF226_02325"
     CDS             11845..12774
                     /locus_tag="VF226_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072850.1"
                     /translation="MLHLVDPENSFVLLALAILTDGLVYAAYLFIVAIGLSLIFGVMK
                     ILNVAHGAFYAFGAYCAATAAGMYFDKGLPEAGGFLAMALAALAVGLVLGLLLERTIL
                     RFVYGKDEVIIVLVTYAAFLILEDVIIIIWGPSSLSAYQPLIAAGNFMLGPLILSGYD
                     LGLVLLAIFLAIGSFLGLRHTRYGRLLTAVIFDRETAAAFGVNVTLFFTITFIIGAML
                     GALGGAVMAPKISVTLGIGVEVIVLAFAVVAVGGMGSIEGALVGALIVGLCRAAAVHM
                     LPQVELFVIYAVMAFVLVFRPHGLFTRAQPRKI"
     gene            12776..13726
                     /locus_tag="VF226_02330"
     CDS             12776..13726
                     /locus_tag="VF226_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072851.1"
                     /translation="MIERRHILGGSLFAASAVVGPFLPPWLISLLTIAFASALVVLGL
                     IVLWRAGLVSFGQALYYAIGAYTVALFMRWTGIHDAFLAVILAALAAALTAFLVGFLL
                     ARYREIFFAMLSLALSMILYGVLVKTEALGSTDGFNVSQPTFLGYAPHGMELSIALYW
                     YALGLGAIAAVLVGAYLRSVAGSLAIPVRDNEIRVEYLGVSATKLIHLKLVIAGTLGG
                     VGGAIGAMAISHVDPNMSYWTTSGGFVFITILAGAGSVAAAFVGAVVFEIVRTMSVAL
                     LPGAWQLILGTVLLLTILFLPDGIGSLFMRRRRKAQEAAQ"
     gene            13723..14508
                     /locus_tag="VF226_02335"
     CDS             13723..14508
                     /locus_tag="VF226_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072852.1"
                     /translation="MTPVISVRNLEKSFGSVVAARDINVEIPALQTVGIIGANGAGKT
                     TFVNMITGHITPSSGSIWFSGKDVTGLPSRTICRMGISRSFQVAQIFPSLTVYENMCA
                     AAAIARNNVSVLRFAVMPLLNPEAEAETEEAIDLFQLKKYRDAPAATLPQGVRKLLDI
                     AMAVAGRPQVLLLDEPTSGISVEEKYDLMEIVMAALKSRKVTVLFVEHDMELVDRFAE
                     RVLAFYDGTVISDGAPAEVLGDPRVQELITGHALSKTGSAVHA"
     gene            14501..15181
                     /locus_tag="VF226_02340"
     CDS             14501..15181
                     /locus_tag="VF226_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7072853.1"
                     /translation="MLEVSGLNVSIGAVPIVHDASFFVGEGEFCGLIGRNGAGKTTLL
                     RALMGALPATGKAVLDGTDLIALPSHHRVRKGIGYMPEDRRLVPEFTVEENIMLPAWA
                     AQMSDAPKQLDWIFGIMPEVARFRSRRALELSGGQQKMVALARALMAGRRLLLLDEPF
                     EGLAPALARRLGEVMADLKAERVSVLIAESNEGHVTDLLVRTFRIERGRVSAQSHQPA
                     SEAAAQSA"
     gene            15391..16665
                     /locus_tag="VF226_02345"
     CDS             15391..16665
                     /locus_tag="VF226_02345"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7072854.1"
                     /translation="MVLSSQQSGKVLPIARLPVVTPGAGFDLLDGVRVLDLTTSVAGP
                     FATMLLADLGAEVVKIERPGVGDDARAWGPPFLDGQSLWFMSVNRNKRSVALDYSQPE
                     GLGILRDLAARCDVVVINQPARVARKLGVDAAAMRAVREDLIHVSITGFGLDGERADW
                     TCYDLIAEGYSGIMDLTGEMDGEPQKIGTPAADMLAGQDAALATVSALFARMKTGKGR
                     TIDISLVDSMTRFLNCRIVPYLGSGELPRRSGGRDSVIAIYQAFHTADEPITLGLGND
                     NIWRRFWEAVGEPAYGADPGFATNADRRAKRAEIVAHIQELLLAKSRAHWLAVFRQAR
                     VPAGPINRIDEVADDPELQRRGLLYCLAADGRHFPQVGTGFCLDGSSNVPRLPPPRLG
                     EHTAAVLSTLLGLSESRLAALRAAGTIAGGTE"
     gene            16662..17441
                     /locus_tag="VF226_02350"
     CDS             16662..17441
                     /locus_tag="VF226_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7072855.1"
                     /translation="MSYETILYEEDGPIGTITLNRPHDGNMFTETMCHEVRDCINAIR
                     RETRTRVVVITGAGDKFFCIGGRKEGMPKTTLYAGTLPTLEMYESIDRLQKPVIASVN
                     GYAVGGGNVLQVVCDLTIAKESAIFRQVGPMMGSFDAGYGTWYLEDLLGKKKAKEIWF
                     RNPKITAREALELGLINKVVPDDKLKEETRAFALEIAERGSFALASVKAAFNARHGGV
                     AGLARMAHDLLLRSYLESEESHELSAAFSERRKPDPSKFGR"
     gene            17445..19067
                     /locus_tag="VF226_02355"
     CDS             17445..19067
                     /locus_tag="VF226_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX7072856.1"
                     /translation="MRPFLTLHHPAAAARYYEQGLWCSETFYTLAARHAQGRPDAIAV
                     QDGRRALSWRELVHWVDGVAADLRTYGLVGGDRVSIWMSNRIEAIVTFLACSREGFAC
                     NPSLHRTYTCAEIGRLLTRLSARALVTEPGWGADRAGTDLNAVLAGVPSIKVVYTPDS
                     FPVGAPNKTPPVEDPDKVTYLAFTSGTTGDPKCVMHSDNTLLANARDLVRDWGHGPDT
                     VILSLSPLSHHIAWVAVAQWVLSGGRLVTDDPPAGMSRLDWIVSTRATYVMGVPTHAM
                     DILAEQRARGMERLGDVRVFYMAGAPIPPSVAQAFVAQSIKPQNVYGMTENSSHQYTH
                     PADETQVIVETCGRGGRAYEIRLFDPGDPDRPVPPGEVGQIGGRGAALMLGYFDNQTA
                     TESSFNRDGWFMSGDLGVLDAQGNLRIEGRLKDLIIRGGHNIYPAQIEALALRHHLVM
                     KAACFPVADERLGERVCIAVIGEIEPDMLLEHLAQEGLSKYYMPEYFLCMESFPLTAS
                     GKILKRRLVEMVRKGELSPIPIRYRSREESRV"
     gene            19064..19831
                     /locus_tag="VF226_02360"
     CDS             19064..19831
                     /locus_tag="VF226_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7072857.1"
                     /translation="MSVKLSKRDEFAILTLDRPEALNALSFAIIDEIANAIDEAAQLP
                     VRALIITGAGEKAFCAGADIKELRGRDLVAQRRGAEKGQAVFAKLDRHPVPSVAAING
                     YAFGGGLELAMACTFRVASPTAKMGLPEIKLGLIPGYGGTQRLPRLIGEARALEMIMT
                     GRTVGAEEAERIGLVNRIVEGDILEGAIAFARSFTCFSLPVLGLARDAVRRALDLPIH
                     EGLRIEADLSTLAFRTADAEEGMAAFVEKRKAEFKDA"
     gene            19824..20531
                     /locus_tag="VF226_02365"
     CDS             19824..20531
                     /locus_tag="VF226_02365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072858.1"
                     /translation="MPEGQIIVTGASRGIGAAIAAELASRGFTVAALSRSGAAAHGYG
                     LVCDMTDEAAVARTIRSIAERGPIVGLVNNAGAQRAEPSATLSAAEFESTMRLNATSV
                     LVASREAYPHLKAAGGGLIVNMGSFFDKLGVARNLAYCASKAAIGAITRCLAVEWARD
                     NISVIDIAPGYIETDLNRDFLASDKIRSWLARRIPVGRPGQPNEVARLVGVLFAERID
                     FLTGETIYIDGGHGMNH"
     gene            20531..21697
                     /locus_tag="VF226_02370"
     CDS             20531..21697
                     /locus_tag="VF226_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7072859.1"
                     /translation="MKVFEQLERRRRWNEEEALILEQVRRVADTVIAPNAEHYDRTGE
                     FPWKNIEALNALGLNGLFVPEAYGGAPMSYRLYLAVVKILAEACASTGIIYATNFHGM
                     KPLIDLGTAEQKARLLPRIASGGLGALAITEPNAGSDAAGMQTRFTPDGDEIIVDGGK
                     TFITNGDVADLILLFGKWSEIEDPRRAISVLILEKGTPGLEVVRLEDKMGHRASSTAT
                     LSFRACRVPRANLIGQPGEGLRILLSSLNKSRPSIAAHALGIAHAAFKDMVGYMNERR
                     QSGQRIIDFQGNQFSLADMATDLAMAEVWLDYVAGLLEDGETDFGLEASMAKMRASDL
                     AMRIATEAVQMHGGYGYCRDYRVERLMRDAKITQIWEGTNQIHRQLIGRSFAAR"
     gene            21694..22575
                     /locus_tag="VF226_02375"
     CDS             21694..22575
                     /locus_tag="VF226_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7072860.1"
                     /translation="MTTEIRTVGVCGAGGTMGAGIAIVAARGGFRTISYDRDAGALDR
                     AAEQTAAFFGKSVERGKLSAEDRDSILARMHRTTRIEDLAACDLVIEAVFEDLEIKRT
                     LFGKLDGICPEHTIFASNTSTLSITEIASGCSRPAKVVGMHFCLPAQLMKLIEMSPGL
                     MTSEETFKAAWEWTEKCGQIPVKTQDKPGFILNALLVPFNNDAIRAVEAGVASPADID
                     KAIKSALGYKMGPLELVDLVGLDTQLRLCEAFYPITLDPRAAAPPLLRRMVAAGLLGR
                     KTGRGFFTYDGAKMFGA"
     gene            22580..23221
                     /locus_tag="VF226_02380"
     CDS             22580..23221
                     /locus_tag="VF226_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7072861.1"
                     /translation="MRYAIINGGVSRSFAENDLLFSSAAAAEDADVLIYADGTKLAPD
                     PAKVAVLVELGTESLAIYTGEEPNTSFANVVGYARYRNGDDAPSKLVELVRRPWTNDS
                     ALQAARDVFEGAGLDVVVCADQPGRIIDRLVRPKYNAALRLLDEGLATEADMDLTCRL
                     GLGYPDGPIERIKRGGLLHHYKITQALFETYGTPAYAPPRQAIDAARRAGVKK"
     gene            23218..24333
                     /locus_tag="VF226_02385"
     CDS             23218..24333
                     /locus_tag="VF226_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7072862.1"
                     /translation="MRPLEGLLVLDFSTLLPGPMATLLLAESGAEVIKIERPGSGEDM
                     RHYKPRWGRDSVNFALLNRGKKSIALDLKNSEQRAKLDPLIARADVLVEQFRPGVMAR
                     LGLDYETLEKVNPRLIYCSITGYGQNGPKRDVAGHDLNYIGDTGLLSLSMGPLDSPVV
                     PPALIADIAGGAYPAVMNILLALQQRARTGRGSYLDVAMSDNLFPFMYWAIGNGLAAG
                     TWPGNGTDLVTGGSPRYRLYPTADRRIVAAAPLEQKFWETFCEIIGLDAELRDDAKNP
                     TATIARITELIAAEPAKIWAERFAGRDCCCSVVATVSEALEDAHFRARGLFSHVLAGE
                     GGQRLPALPVPIASIFRGAPGVPVPAPGLGANNEDYE"
     gene            24330..25328
                     /locus_tag="VF226_02390"
     CDS             24330..25328
                     /locus_tag="VF226_02390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX7072863.1"
                     /translation="MKAVLVRDFAPFTQVGVAEVDDPVPAPGEVIVDVVAAEANYPDI
                     LVIEGKYQVKPPLPFSPGKAAAGRVAALGEGVRNFSIGDPVAVQVEYGAYAEKVRARA
                     ESCFRIPDGMPFEKAAALGLVYQTAWFALKDRASFRTGESVLVLGASGGIGVASAQLA
                     KAFGAGAVIAGVLGESNAEVARRAGADHVVDLGRADLKDSLKAEVAAVTDGRGADVVI
                     DPVGGAANAAALRAMAWKGRLVVIGFASGEIPTIRANYLLVKNISVAGLQWSDYRDRT
                     PELVVAAQKEIFDLYTRGLIDPVISRTVPLEDFKTALALLRDGQAQGKIILQVRQA"
     gene            26572..26814
                     /locus_tag="VF226_02395"
                     /pseudo
     CDS             26572..26814
                     /locus_tag="VF226_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT         5098 a         8190 c         8592 g         5222 t
ORIGIN      
        1 tctggcgaac ggtgccacga cgctgatgtt cgagggggtg cccaactatc cgacgccgtc
       61 gcggttctgg cacgtcgtgg ataagtgggg ggtgaacatt ttctacacgg cgccaacggc
      121 gatccgcgcc ttgatgggtg ccggcgatga tttcgtgaag cgcacggaca ggtcctcgct
      181 gcggctgctc ggtagcgtcg gcgagccgat caatcccgag gcgtgggagt ggtactaccg
      241 tgtcgtcggc gaaagccgct gccccgttgt cgacacgtgg tggcagacgg agaacggcgg
      301 cattctcatc tcgcctctgc ccggcgctat gaagctcaag cccggctcgg cgacgctgcc
      361 gtacttcggc gttgtgcccg gcatcgttaa cgacaagggc gagctgatcg aagggcccgg
      421 cagcggcaac ctggtgatcc tcgacagctg gccgggacaa atgcgtaccg tctacggcga
      481 tcacgagaga ttcgtccaga cgtacttctc gacctacccg ggcatgtact tcagcggtga
      541 cggctgccgt cgcgacgagg atggctatta ctggatcacg ggccgcgtgg acgacgtgat
      601 caacgtttcc ggccaccgca tgggcacagc ggaaatcgag agcgcgctcg tcgcgcatcc
      661 gaaggtggcc gaggcagccg tcgtcggcta tccgcatgac atcaaggggc agggcatcta
      721 ctgctacgtg accctgctcg ccggagagac gccaagcgac gagctcaaga aggagctggt
      781 cgatcacgtg cgcaaggaga tcgggcccat tgctgccccg gatctcatcc agttcgcccc
      841 gggtctgccg aagacgcgct cgggcaagat catgcggcgt attctgcgga agatcgcaga
      901 ggacgacttc tccaatctgg gcgatacctc gacgctcgcc gacccgagcg tcgtggacga
      961 tctgatcaac aaccgccaga accgccgggc tggctgatcg ccccaagggc agaatgagct
     1021 gaaggcccgc cctgcagggc gggccttcat ttctttcgtg gagacttgtg ccctcacttg
     1081 cacgtcgaaa gaatgccgcg gtcgaggtga aaatgatctc gatgtgcgcg attgaactcg
     1141 gggctcagcg ccacgcggaa gtagttgcag gctccggcgt ggacgagcct taggaactcc
     1201 gcttccggtg tctggccgcg ccagtccttc agcacggaaa tgcgctttcc gtcctccagc
     1261 gtgaatgcgg agatgtcgat ggcattcgcg gttgcgtgct cgctgcgctt gttcttccag
     1321 aacgcgttgc cgacgatgtt gcggcacgaa taggtgccca tgtgctcgag cgacttcacc
     1381 cgctggccga acatggactg ggcaagcggc tgcacctgat gaaccatcca caagctgagt
     1441 gcggccgcag ccgggcagga gagcttgtcg acgggcagcc tggcgccgcc gaccgacgaa
     1501 acacgcacgc tgttgatcca gccacagccc ttgttcaatg ggttatcgcg aatgggcacc
     1561 gattcgatat gcggcggaac gagcacgcgg gagcagagct ttgcatcgta tctcagctcg
     1621 gccagctgcc agtcgacgat cagcggcaga ggacgttcga gatcaagggc gggcaacgga
     1681 ctatatcttg ccggcaccag cccctgccgg aaagccagcc cgattcctgc aagcagcatc
     1741 agcagcagga gataccgtcc aattttcaat cggcgtttgc gcatgaggat cgctcggcgg
     1801 cggggtatcc cctctatagg ggctttcgtg cggcatgtaa gcaggggtga tgcaccagca
     1861 gcgtggcagt tgcaccgatg ccattcatgt tacgcagcac ggaacgagca ccgggcatgt
     1921 ctcgcaagcg atggcatttt ccggccaagc ccgatccgat cacggccgat ccatgcaagc
     1981 ggggcggccg tttaggtgtg cgtcaccaaa tcggttttgg ctgctggtca aaccggcggc
     2041 gcaatggcat tgagaccgcc cataatgaac cggatacaac cggggcccat gctgttcgtc
     2101 accagtgccg agaaataccg ctccgaggcg gagggctggc tcagtcgcgc tgcacgctat
     2161 cctgagggtt gctgggaccg cgagcgcctg atcgtggccg cacggctcaa tcgtgcgctc
     2221 gctgatagga tcgagcgcag ttgcagaccg acaggcgagc ggcagctcag ttgctcgttt
     2281 gaccgtcctg ctcggttgcg ggaggcactc cagccaactt ctgtggagaa agccgggctt
     2341 cccgcagtga ccagacgtgg tcggccccgc aatgcgggca acgcgtcttc atcttgatgt
     2401 cgggcaggcg gctgaaactg gcttcatcga tttcgatgtt gctcgcgtac tcctgacccg
     2461 tgaccggaca gcggaagaag attgtgccca tggcgtctgt cctctgggca gccacttgct
     2521 catcaaaccg tagtgacaat ctgtctcagt cgcaaatccg gaacaatgag cagcgatcac
     2581 tttgtcgtgt cttccccgta acaaggctaa aggcggtctt ggatcgatcg catccggggc
     2641 gcagtgtctc gatttttcca cgttccctcc ggaacagcgg gatttcgagg cttcgggcac
     2701 gatcaggcta tccgatcgat ggagatagcc atgaccatga tgtcccgcac ctctgctggc
     2761 cgactcgcaa ccgtaagcac catcgctctc gtcaatattg tcggcattgc cgccatttcg
     2821 agttctgcca aggccgtcag ccttcgcgtg cagatggcct gcgcgagcga ctacttctcc
     2881 tactgcagca agtatccgac cgaaggcccc caggtccgcc ggtgcatgcg cgccaacggc
     2941 cacaaactgt cgcccgcttg cgtcaacgcc ctcgttgccg ccggcgaagt ctccaaggct
     3001 gaagtcgcgc gtcgctccgc aggggcgaac tgaatacatc tctggggcgg gttgccgaag
     3061 cgtagctcgt ccttttctag agggcgccag caatcatagc catcgattcg ccgcgtacgg
     3121 gcaattgaaa gggtcgatcc tcctcacgat atgcccccgg gggagattgg tccttgtttc
     3181 agcgagcgga ttgaggacag ccatgactga cgccaccgcg acgaccccca tgcaatatct
     3241 cgatcgcgcg acccgccatt tgcgcgacat cggcttgatg ccggcgaagg tcgagccggc
     3301 accgataaac gcgcttctgg agaagatttc cgatctcgac gaggagcgga tcacgcttat
     3361 tgcgcgcacg ctcggccagg cggaggtgtt caacgaggtg gtgcgccagg aaacggctgg
     3421 gatggcgatc gggcagcgct atcaggaaat cacggatggc ttcaactcga tccgcgacga
     3481 tgcaaggcgg ctggtcgatc aggtgagtga cggcaagctc aacatcttcg agcgcggcag
     3541 caatgtctgg atgaagatcg tgcgcgggga tatcgccgac cggttcgaaa aaatccggca
     3601 ggtctatcta gcggtcaccg cggagacgaa gaaccagatc gagcgggaag cgaagattct
     3661 ggacgcctac agtgattttc gcggcgcact caagcacgcc gaggtgatgg ccctcgaggt
     3721 tctcaagaaa gcggaagaca agctcgaggc cgcacgcaaa cgcctggacg aggcctccgc
     3781 caaggtggcc ggtttcaccg gcacggagcc ggccgagcgg gccaagctgg agcttgcccg
     3841 cgacgagcag cttcgggcgc ttcaggaaga ggagaagcgc tatcagatcg caaaggacct
     3901 ctcggacaac ctgacgatcg cctacaatac gtccgaggta atcatgggac gcctgatgca
     3961 gaccacctcc gccaaggagc gggtctatgc gcaggcggtt tcgttcttct cgacgaacga
     4021 cagcgtgctg acggcgctga aggcgtcgtt caccggcgtt ttcgggctgc acgagtcgac
     4081 caagacgctc gaagccatga aggaggggat gtcgaagagt ctcgaggacc tcgccaagct
     4141 cggagacaag gtccaagaag aggcgctgcg ggccggctac ggccccacga tccgtgcaga
     4201 tgccgtcaag cagctcatcg acagcgtcat caatttccag gagcgctcgc gcgagatcat
     4261 caacgagatg cgcaagctcg ccacgcagaa ctcggccgag atccgcgaag ccgtcgagga
     4321 tggcaagcgg cgcatcgccc gtctggctgc cgaggcacag cttctgccgg cccggcattg
     4381 agttctgagg accatagggg cgaatggtga gcgacactgc ggtcgagccg aagccgcata
     4441 cgctggacga attgatgatc gcgatggacg tggtcgacac gctgcgccat cgcgaggatc
     4501 tcatacgccg cgagctcgac gaggcgggac gcgaagcgga gttgatcgcg cggctgaagg
     4561 aaatctatca gcagcagggg atcgatgtcc cagaccgcgt gctggagcag ggcgtcaagg
     4621 ctctcaagga aagccgcttc gtctacactc ccccgccgcc ctcctggaag cgcacgattc
     4681 tcgaggcctg ggcgcggcgg ggcgtcatcg gcaaacgggt cctggccaca cttgccgtcc
     4741 ttctgatttt ggcgggcgtt gtttacttcg ggctcgtccg tccggtgcag caggcgcggc
     4801 aacaggcctt gatcgaggcg accgaaacac ttccaagagc gatccggaca gcccattccg
     4861 acatcgcgca gattgcaacg gacgaggcgg cccggagcag ggcggatgcg ctgctggctg
     4921 acggcgagcg ggccattcgc gacagggatg tcgcgggcat gcgccgcgcc aacgccgagc
     4981 tcgcacagct tctggatacg gtgagcaggg aatacaccct gcggatcgta tcggagcctg
     5041 gcaagctcac aggactgtgg ctgagcccgc cgggctggtt caaggaacgc cggtactatc
     5101 tgatcgtcga gccggtcacg cccgacgggc agaagctgac gctgcccatc cgggacgagg
     5161 tgaccggcga aacgaaaaac gtcagtcaat tcggcgtccg cgttccccgg aagacctatg
     5221 aagcggtcgc agaggacaag cggccggacg gaatcgtcga caacaacctg ttcggcgtaa
     5281 agctccgcgg caggctgcag gtcgactacc gcatgccctt cgagggcgga accatcaaca
     5341 cctggtagcg ggcagcgtcg atcgatgtgg acaggacgtc aggcgttcga gcgcatcgag
     5401 aacgcaatcg tcggcctcca tcggcaggag accgagctcg acagcgcgct cgcctcagcc
     5461 gcggccgatg ccgagcgcct gcgccgcgag cggagcgacg ccttccgcga gctcgcgcgc
     5521 atcaagctcg acgagataac ggctcagcgg ctggtcgatg atctcgacgc ggcggaaacg
     5581 cgcgctgttc agttgctcga gggtcgccgg cgacagctcg aatcgatcac cgcccagcgc
     5641 gaacaggcca tcatcgccct ggagcgggcc gaggcggaac gcgctgaggc cgctgccgca
     5701 ctcgagcggg ccatagatgc cctcgacgag ctacgctcca aggtggcgga cgagatccgc
     5761 tcatcagaag aatggaatcg cgcacgcgct gcgctcgaga aagcgacgaa ggtggccgat
     5821 gcggccgacg ccaaggccgc actgtcggag gccgagctga gtgagaagcg ccaaccctac
     5881 gacaatgatc ctctgttcgc ctacctgtgg cacatcggct atgggacccc gcgctatcgg
     5941 gccggcagct ttgcgcgctt catggaccgc atgactgcgc gcttcatcgg tttcccggac
     6001 gctcgcctca actacgcgat gctgactgaa attccagcgc ggctgaggga gcacgcacgc
     6061 ctgcagcgcg agattgcgga cgaacggcgg gctgagctgc tggccatcga gcagaaggcg
     6121 atcgccgcag ccggcgaggg gaccttgtca cgcgaggtcg aggaggcgcg ccgcaaattg
     6181 gcagcggccg acgaggcgat cgagagccgg caatcggagc tccgcaacct cgaccagaag
     6241 cgcgatgcac tcgtcgccgg ggtcgatgat cccgcctatc gcgaagcctt gcagacgatc
     6301 gccagcgccg atgcgcagga tcagttcgaa aacctggtgg cggaggcgaa gcgcacggcc
     6361 acggaggccg atgacgcgat cgtgcgccgg atcggtgatc tgaacgagcg gatcgacagg
     6421 atcgaagcag agatcgccga gctgcgcaaa tccgcacgcg cgattgcgga acggcgggtc
     6481 gaggtggagc gcgcacgcga ccgctttcgc gagtccggct acgatcatcc ggacgccacc
     6541 ttcgacaacg acgtcgatct cgaccgaatt cttcggcagg ttctcggagg ggtcgttacg
     6601 ggtggcgtac tctgggatct gctccggtcc ggcttcggcc tgcgctatcc gggtggccgg
     6661 cggtccggcg gcctttcgtt cccgttcccg tttcccattc cgatgccgga ccgccgggag
     6721 gatacccgcg gcggggattg gcggcgggcg gactcacggg gcggctggtc tccgactcac
     6781 gagtcttgga aaaggagccg ttgggaccgc gacgacgatg atgacgatga cgatcacgat
     6841 cacgggcatc gcggcggctg gaggacgggc ggccgattct gacctgcaac ttccatgggc
     6901 gatattcgac cattcggcag gccctgtaag cggtccactg aattgagctc gtgaggggcg
     6961 cggggcctcc caactgttcc agccaggtct atggcgaaaa agtctctttc cggagctcac
     7021 tgcgccaggc cgtctttctc caccatctcg gccgttgacg aacactgcca gttgtgtgat
     7081 cagtgtagag attgcatgac cggctactaa aggccggaat tgccttgttg cagaccgatt
     7141 gattttcgag tcgatttgaa ttggagggcc aaatggaggc cactcggcgg agtgcgcttc
     7201 agctcattct cgcgagcgga ttactttctt gttttcccag aattgccgtt gcaggtcccg
     7261 ccgtttgccg gactgggatt ctgacgagcc atcgcctcgg taacggcttg cgggtgcatt
     7321 gctatagaaa cggctcgaag tatgtcagcg ccgcgctggt gctgcggagc tgcgagattt
     7381 tgaaccacag cggcctcgcg cacatcctgg agcacacgtc gttcaccggc gcggcgggaa
     7441 actactcggc caaggagctc aagcgacggc acaggacctt cattcaggac agcaacgcaa
     7501 gcaccagtcc cggcaggctg gaatggtatg cgtcgtttct gcccaaatac gctccagagg
     7561 ccatcgagct cctcgcgatc acaagcctgg atcagaaatg tgacctgaga acggttgaga
     7621 gtgaagccaa aattgttctt caagagcttc tactggataa gcatagcggc gatgctgcga
     7681 tccgaaggca gttcaatgct gccctctatg gcgaaaatca tccctacgcg gtcgataact
     7741 gcgacgcgga aatcgccaag gtcaaacttc cagccagaga gttggcgaga gagctgaccg
     7801 aatatgcgag cctcatccga cagccggcca atatggatct cgtcttggtc ggtgacgtcg
     7861 agacgggcga catttcccaa ctcgttgaga agtgcttcgg ctgctacccc catgcgcaag
     7921 gaccggtatt ggagataccc aaagcgcaac cgactcgcga atatcggagt atcgagggcg
     7981 tgtccggaga tctcgagcgt cctctgagcg aaatccgtat cggatggaat acaggagtga
     8041 ccataaatga tccggacgct cgcgtgctcc tgacacttgg tgcatatctg aacgacgtga
     8101 tcttttcaga ggtgagggag aggcacggcg actcctattc tcccgaagcc tattttgaac
     8161 cggatggttg cagcggaatt ttcacgatca gcgccataac gagcaagcat ccggagttgg
     8221 ttgaagagcg aatcttctcg gccatcgagc gcgtgaagga ggagatcgat ccgctcgagc
     8281 tcagattgca caaagagcgt tgggagctca agcgcctcag aaccgcggaa agtggcagct
     8341 cgatgctcga atgtctggtt gcccgcattg tcgagggctg tgcggtcgag gatctcgaca
     8401 tcgagagcgt gtcgccagcg gagatggtgg aggcagcccg caaatatctg cccgactaca
     8461 aaggcgcgta tacgcgagtg acgctgaatg gcgcctgatt tcgcttacgt cttcatctcg
     8521 ccaaggatct tgtacaggta ggagcgcgaa atcccgagct cctgggcgac gcgcttcttg
     8581 tttccattat ggcgacgcag cgctttcaga acgagatcgg tctcgatccg actcaagcgc
     8641 tcgcgaagcg tcgtagcggc gcttgcttcg ggcatcggtc gggaaaagcc gttcgactgc
     8701 ggagcaaacg actccgcggt gattatattg tcatcggcga agatgagggc ccgttctacc
     8761 gcatgccgga gctggcgtac attccccggc cagctctgtt ccatgagcca cgacagagcg
     8821 gactcggtga tctcgggtgc cggccggcga tggcgtaccg aaaagtcggc gacgaaatgc
     8881 gcgacaagtg ccgggatgtc gtcgaggcgc cgtttcagcg gagggacctc gatcgtgatc
     8941 ggggaaatcc ggtaatagag atcaagccgg aatttgcctt cggagacgaa ctgctgcagg
     9001 tcacggttcg tggcagttac caagcggaaa tcaacctgct gcggccggtc gctgccgatc
     9061 cgctcgatca tgcggtcctg caggaccctg agcagctttg attggacctc gatcggcatg
     9121 tcgccgatct cgtcgagaaa tatcgttccg cctgccgcct gttcgaactt gcccttccgg
     9181 cccttgcgcg cggctcccgt aaacgcgccc ggctcgtatc cgaaaagctc cgactccacc
     9241 agtgttgggg ggagggcggc ggcgttcacc atgacgattg ccgcgtcgcg acgcgggctc
     9301 agcctgtgga gcgcttgcgc caccagttcc ttgcccgtgc cgctctctcc gcggatcagc
     9361 acgggaattt ccaatggggc aaccttgacg atctcatcgc gcagccggcg catggcaggg
     9421 ctatcgccga tcagctgatc caggccgtag ctcttgctgc gcagcgccgc ggtctcccgc
     9481 ttgtagaact cgacctcgct ctcgagagca tggattcggc gtcccagctc ctccacctgc
     9541 tggggccctt tgaacatcac acggccaacg gcacctaaaa tcctgccatc cctccggatc
     9601 gggatccggc tcacgacccg gtccagcccg cgcattctct ggatctctcc gacctcggcc
     9661 ttgccggtgg cgaccacctg gtcgaggcgc gtgttctcga tcacctcctt gaccggcttg
     9721 ccattggcct cgccgcgctg cagaccgaaa aagctctcgt gaaccggcga gatgtaaacg
     9781 agccgcgcat cggcatcgac gatggtcatc gcttcgaacg ggtcggtgag catgtgctcg
     9841 aggatgtcgt aggcgaattc cacggagccg atgaattcca ggagtgcatt gcttttcgga
     9901 cggctgatga caagaagatc gcccccgctc atcggcacgt cgagaatggc gtacgagttg
     9961 cggtccagga tcagaggttg gagcctgcgc tcgcggattt cggctctcca agcctgatcg
    10021 gaaaccaaag ccttgacccg ggaatcgttg ccgatcccgg acgcgaacag cacgtttcca
    10081 gcttcatccg cagcaacgac gccctcgata ggggtgccgg atgcccccat cccttcctcc
    10141 ctgcacgcga tagcgacaat gttgacgcga gctagaaagg tatgcaacct gaatctgtcc
    10201 tcgacaaaaa cactccgtcc tgaaccgctg cttgagtttg ccagggtcga tgtgtacgcg
    10261 aagaggacac gtttgccagg gcgaccgtcc acggtctgga catccggagg gccgcttcga
    10321 ccatcatcgg cagcaaaagg cttacggatt gagggtttgt tttgatatag gttgcagtgg
    10381 cacgactgtt gcttgcctaa tgtcagcgag tgccctcgca tttcacaaag ggagacacgc
    10441 catggggctt agatttggga tcggcagact gtcagcgata ggcgccgggg cggcgctgtc
    10501 ggttgcgcta acagcttctg cctatgcaca ggagccggtg aagctcggaa tcgtttcgtt
    10561 cctcaccggc ccggcggcgg tgccgttcgg tgttcccggc cgcaacgcgg ccgagctgat
    10621 catcgacgcg atcaacaagg gctccctgcc tgccccctac aacagcaagg ggctcggagg
    10681 cgccagcatc gaggcccgct atgtcgacga ggccggttcg accgccaacc aggtgaccga
    10741 gtaccgcaac ctcgtgcagc gtgacggtgt cgatgcggtg gtgggctacg tatcctccgg
    10801 caactgtctg gccgttgcac ctgttgccga ggaaatgaaa acgatcacgg ttctctacga
    10861 ttgcggcacc ccccgcattt tcgaggaaga cccaaaccac aaatatgtgt tccgcgtgac
    10921 gccgacggcg acgatggata gcgtcggcgc agcacgctac gtcgtcgaca agaagggcga
    10981 tatcaagagc ttttccggcc tcaaccagaa ctacgcctgg ggccaggatt cctggcgcga
    11041 tttcgtgctg gcgatgaagg ctttggcccc gaatgcgact gtcgaccgcg agttgttccc
    11101 caagttcctg gcgggcgagt acggtgctga gatcacatcc ttgctgacct ccgaggccga
    11161 gctggtgcac agcagcttct acggcggcga cctcgagagc tttgtcgccc aggcgaccgg
    11221 gcgcggtctc tcacaacgta agacgatgat cttctcgtgc ggggaatcgg cgatgttccg
    11281 ccttggcaac aggatgcctg acggaaccat catcggtgga cgcggtccgc acggcgtgct
    11341 ggcccgcgaa tcggagctga atacctggtt ccaaaaggaa tatgccgcgc gcttcggcac
    11401 gccgccgacc tacccgtcgt accatatggc ccagtcgatt ctcggcttga aagctgcttg
    11461 ggacaaggct gctgctgcga agaatggagc acgccccagc accgacgaaa tcatcgcggc
    11521 gttcgagggc ctggagttcg acggtccgag cacgcggatc tcgatggcgc gtgccaatgg
    11581 ccaccagggc atctccgaaa cggcttatgg cgtcttcaag ttcaacaagg aagccaatcg
    11641 tcccgaggtc gtggacatcg tgcgctaccc cgccgagtgc gtgaatccgc cggacggcgt
    11701 cacctcccag gagtggctcg agggcggcat gaagggcgcc aagtgcgact gagtggatcg
    11761 cttccttcgg aagtgacgcg atagcacttg tccgatctgg actgagttcc gcgcctcgcc
    11821 ccaagtgacc agcgaggcgc ggaattgctt catctggtcg acccggagaa ctcattcgtg
    11881 ctgttagctc tcgccatcct gacggacggc ctcgtttacg ccgcgtatct tttcatcgtc
    11941 gcaatcgggc tcagcctgat tttcggcgtg atgaaaattc tcaatgtcgc ccatggtgcg
    12001 ttctacgcat tcggtgccta ctgcgctgcg actgccgcag gcatgtactt cgacaaaggt
    12061 ctgcctgaag cgggcggctt ccttgccatg gcgctggcgg cgctggctgt cggcctcgtg
    12121 ctggggcttc tgctcgagcg gacgatcctc cggttcgtct acgggaagga cgaggtcatc
    12181 atcgtcctcg tcacctatgc tgccttcctc attctcgagg atgtcatcat catcatctgg
    12241 gggccgtcct cgctttcggc ttatcagccc ttgattgccg ccggaaattt catgctgggg
    12301 ccgttgatcc tttcaggtta cgacctcggc ctcgtgctgc tcgccatttt cctggccatc
    12361 ggcagcttcc tggggctccg ccacacccgc tatgggcgcc tgctgaccgc ggtgattttc
    12421 gatcgcgaga ccgctgccgc attcggggtg aacgtgaccc tgttcttcac gatcacgttc
    12481 atcatcggtg cgatgctcgg cgccttgggt ggcgcggtca tggcgccgaa gatctcggtg
    12541 acgctcggga taggcgtcga ggtcattgtg ctcgccttcg ccgtcgtcgc ggtcggtgga
    12601 atggggtcga tcgagggcgc cctcgtcggc gcgctcatcg tcgggctctg ccgcgcagcc
    12661 gcagttcaca tgctcccgca ggtcgagttg ttcgtcattt atgccgtgat ggcgttcgtc
    12721 ctcgttttcc ggccccacgg tctgttcacg cgcgcacaac cgaggaagat ctgagatgat
    12781 cgaacgtcga catatcctcg gcggatcgct gtttgccgcg tcggcggtgg tcgggccgtt
    12841 tctgccgccg tggctcattt cgctgctgac gatcgcattc gccagcgcgt tggtggttct
    12901 ggggctcatc gtgctctggc gggccgggct cgtgtcgttc gggcaggccc tctattacgc
    12961 gatcggcgcc tacaccgtcg ccctcttcat gcgctggacc ggcatccatg atgctttcct
    13021 cgcggtgatc ctcgcggcat tggcggcggc gttgacggcg tttctcgtcg gcttcctgct
    13081 ggcacgctat cgcgaaatct tctttgcgat gctgagcctc gccctgtcga tgatcctcta
    13141 tggcgttctc gtgaagacgg aagcgctcgg ctcgacggac gggttcaatg tctcgcaacc
    13201 gaccttcctc ggctatgccc cgcacgggat ggagctctcg atcgcgcttt actggtacgc
    13261 cctcgggctc ggcgccatcg ccgcggtgct ggtcggggcc tacctgcgct ccgtagcagg
    13321 ctcgctcgcc atacccgtgc gcgacaatga gatccgcgtt gaatatctcg gagtctcggc
    13381 gaccaagctc atccacctga agctcgtgat cgccgggaca cttggcggcg tcggcggtgc
    13441 gatcggcgcc atggcgattt cgcatgtcga tccgaacatg tcctactgga cgacatcggg
    13501 tggcttcgtc ttcatcacca ttctggccgg ggccggatcg gtcgccgccg cattcgtcgg
    13561 cgctgtcgtt ttcgaaatcg tccggaccat gtcggtggca ctcctgccgg gagcctggca
    13621 gctgatactc gggaccgttc tcctactcac cattcttttt ctgcccgacg gcattggctc
    13681 gctcttcatg cgccggcgtc ggaaagcgca ggaggctgca caatgacacc ggtcatctcg
    13741 gtccggaacc tggagaagtc attcggctcc gtcgtcgcag cccgcgatat caatgttgag
    13801 attccggcac tccagaccgt cggcatcatc ggcgccaacg gggccggcaa gacgacgttc
    13861 gtcaacatga tcacggggca cattacgccg agcagcggga gcatctggtt ttctggaaag
    13921 gacgtgaccg gcctgccctc gcgcacgatc tgccgtatgg gcatttcccg atcattccag
    13981 gtggcacaga ttttcccgtc gctgaccgtg tacgagaaca tgtgcgcggc tgctgccatc
    14041 gcccggaaca atgtcagcgt tctgcgcttt gcggtcatgc ctctgcttaa tccggaagcc
    14101 gaggcggaga cggaggaggc gatcgatctt ttccagctga agaaatatcg cgacgcgccg
    14161 gcagcgaccc ttccgcaggg tgtccgtaag cttttggaca tcgccatggc cgtagccgga
    14221 cggccgcagg tgctgctgct cgacgagcca accagcggca tttcggtcga ggagaaatac
    14281 gatctgatgg agatcgtcat ggccgccctc aagagccgga aggtgacggt tttgttcgtc
    14341 gagcacgaca tggagctggt ggatcgcttc gccgagcgtg tgctggcgtt ctatgacggc
    14401 accgtcattt ccgacggggc acccgccgag gttcttggtg acccccgcgt gcaggagctg
    14461 atcaccggtc atgctctttc gaaaacgggg agcgcggtcc atgcttgagg tttccggcct
    14521 gaacgtctcc atcggcgccg tcccgatcgt tcacgacgca tcgttcttcg tcggcgaggg
    14581 cgaattctgc gggctcatcg gccgcaatgg cgcaggcaag accacattgc tgcgcgccct
    14641 catgggggcc cttcccgcca ccggaaaggc ggtcctcgat ggaacggacc tgatcgcgct
    14701 gccgagccat catcgcgtcc gaaagggcat tggctacatg ccggaagacc ggcggctggt
    14761 gcccgagttt acggtcgagg agaacatcat gctgccggcc tgggccgcgc agatgagtga
    14821 tgcgccgaag cagctcgatt ggatattcgg catcatgccg gaggtcgccc ggttccgttc
    14881 gcgccgcgcg ctcgagcttt ccggtggaca gcagaaaatg gttgcgctcg cccgcgcgct
    14941 gatggctggg cggcgtcttt tgctgctcga cgagccgttc gaagggctcg cgccggcact
    15001 tgcccgccgc cttggtgaag tcatggctga tctcaaggcc gagagggttt ccgtcctgat
    15061 cgcggagtcg aacgaagggc acgtcaccga tctcctggtt cgcacattcc ggatcgagcg
    15121 cggacgtgtt tctgcccaat cgcaccagcc cgcctcggag gcggcagctc agtcggcgta
    15181 ggcaattttc ggctggagcc actttttgtt tgatgagagc aaccagagcg tgtcgccgac
    15241 gcggacacgt gaggctcctg acctgatcga ggcggcgaca gttgccgagg cgagcctgtc
    15301 cgttctcggc ctggattcgc caatttgagg gggaatcgct gactggcacg ccggttgctc
    15361 agaagaacgt aaaccatttc gagagggtcg atggttctca gcagccagca atcgggaaag
    15421 gttctgccca tagcacgact tcccgtcgtg actcccgggg cagggttcga tttgctcgac
    15481 ggtgttcgcg ttctcgacct gacgacctcg gtcgctggcc cgtttgccac gatgctgctc
    15541 gccgatctcg gcgccgaggt cgtcaagatc gagcgtcccg gcgttggcga tgatgcacgg
    15601 gcgtggggtc caccgttttt ggacggccaa tcgctgtggt tcatgagcgt gaaccgcaac
    15661 aagcggagcg tggcgctcga ttattcacag cctgaaggtc ttggaattct cagggatctt
    15721 gcggcgcggt gcgatgtggt cgtcataaat cagccggcga gggtggcacg aaagctgggc
    15781 gtcgatgccg ccgcaatgcg cgctgtccgc gaggacctga tccacgtttc cataacgggc
    15841 tttggccttg acggggagcg tgccgactgg acctgctacg atctcatcgc cgagggctat
    15901 tccgggatca tggatctgac cggcgagatg gacggagagc ctcagaaaat cggtacgcct
    15961 gctgcggaca tgcttgcagg ccaggatgcg gcactggcca cggtctcggc tctgttcgcc
    16021 cgcatgaaga ccgggaaagg ccgcacgatc gacatctcgc tggtcgactc gatgacgcga
    16081 tttctgaatt gccggatcgt cccttatttg ggctccggtg agctgccccg gcgatcgggt
    16141 ggccgcgaca gcgtcatcgc catctatcag gcatttcaca cggcggacga gccgatcacg
    16201 ctcggtctcg gcaatgacaa tatctggcgg cgattctggg aggccgtggg cgagcccgcg
    16261 tacggcgctg atccgggttt cgcaaccaat gccgatcgtc gcgcgaagcg cgccgaaatc
    16321 gtggcgcaca tccaagagct gctgctagcc aagtcgcgtg cgcattggct ggcggtattc
    16381 agacaggcac gcgtgcccgc ggggcccatc aaccgcattg acgaagtcgc cgatgatccg
    16441 gagctgcagc gtagggggct gctttactgt ctggcggcgg acggccggca tttccctcag
    16501 gtcgggacag gattttgcct cgacgggagt tcgaatgtcc cccgcttgcc gccgccgagg
    16561 ctcggggagc atacggctgc agtgctcagc actctgttgg gcttaagtga gagcaggctg
    16621 gctgcgcttc gcgctgctgg caccattgcc ggaggcacgg aatgagctac gaaacgatcc
    16681 tgtacgaaga ggatgggccg atcggcacca ttacgctgaa ccggccgcac gacggcaaca
    16741 tgttcactga aaccatgtgc catgaagtgc gcgattgcat aaatgccatc cgccgcgaaa
    16801 cgcgcacacg ggtcgtggtg atcaccgggg cgggggacaa gttcttctgc atcggtggcc
    16861 gtaaggaggg tatgccgaaa acgacccttt atgccggaac cctcccgaca ttggaaatgt
    16921 acgaatcgat cgatcggttg cagaaaccgg ttattgccag cgtcaacggt tatgccgttg
    16981 gcggaggtaa tgtcctgcag gtcgtgtgtg acctcaccat tgccaaggag agcgccatct
    17041 ttcggcaggt agggccgatg atgggctcgt tcgatgccgg ctacgggacg tggtatctgg
    17101 aagacctcct cggaaagaaa aaagcgaaag aaatctggtt tcgcaatccg aagatcacgg
    17161 cgcgcgaggc tttggagctc ggcctgatca acaaggtggt gccggacgac aagctcaagg
    17221 aggaaacccg cgcgtttgcc ctcgaaatcg ccgagcgcgg ctcattcgcc ctcgcatcgg
    17281 tgaaagccgc attcaacgca cggcacggcg gcgttgccgg attggcgcgc atggcgcatg
    17341 atcttctgct gcgctcctat ctcgagagcg aggaaagcca cgagctttcc gctgcgttca
    17401 gtgagcgtcg caagcccgat ccaagcaagt tcggcaggtg agctgtgcgc ccgtttctga
    17461 cactccacca cccggctgcg gcggcccgct attacgagca gggactatgg tgctcggaaa
    17521 cattctacac cctcgccgcc cgtcacgcgc agggccgccc tgatgccatc gccgtgcagg
    17581 atggccgccg ggcgctgtct tggcgagaac tggtccactg ggtcgacggc gtcgcggccg
    17641 atctgcggac ctacgggctt gtgggcggtg atcgcgtatc gatctggatg tcgaaccgta
    17701 tcgaggcgat cgtcaccttt ctcgcctgct cacgagaggg ctttgcctgc aatccatcgc
    17761 tccatcgcac atacacttgc gccgaaatcg gccgccttct cacccgcctt tccgcgcgcg
    17821 cattggtgac cgagccgggc tggggcgcag accgggccgg aacggatctc aatgctgttt
    17881 tggccggagt gccctcgatc aaggtcgtct acacacccga tagctttccc gtcggagcgc
    17941 caaacaagac gcctccggtc gaagatcccg acaaggtgac atatctcgcc ttcacctcgg
    18001 ggacgaccgg cgatccgaaa tgcgtcatgc attcggataa tacgcttctg gcaaacgcgc
    18061 gggatctcgt gcgcgattgg gggcacggtc cggacaccgt cattctctcg ctgtcgccgc
    18121 tgtcacatca catcgcctgg gtggcggtcg cgcagtgggt cctctccgga ggccgattgg
    18181 tgaccgatga tccgccagcc gggatgagcc ggctcgactg gatcgtatcc acgcgcgcga
    18241 cctacgtcat gggcgtgccg acgcacgcca tggatatact cgccgagcag cgtgcccgtg
    18301 gcatggagcg gctgggggac gtgcgggtgt tctacatggc gggcgcgccg atcccgcctt
    18361 ccgtcgcgca ggccttcgtt gcccagtcga tcaagccgca gaacgtctac ggcatgacgg
    18421 aaaactcctc gcaccagtac acccatcccg ccgacgagac gcaagtgatc gtcgagacgt
    18481 gcggccgggg aggacgcgcc tacgaaattc ggctcttcga tccgggcgat cccgaccgtc
    18541 cggttccccc gggcgaggtt gggcagatcg gcgggcgcgg tgcggccttg atgctcggct
    18601 atttcgataa tcagacggcc acggaatcga gcttcaatcg cgacggctgg ttcatgtccg
    18661 gcgatctcgg tgtgctcgat gcgcagggta atctcagaat agagggccgc ctcaaggatc
    18721 tgatcattcg cggcggccac aacatctatc cggcgcagat cgaagcgctg gcgcttcgac
    18781 accatctggt gatgaaggcg gcctgcttcc ccgtagccga cgagcgcctg ggcgaacggg
    18841 tgtgcatcgc cgtcatcggc gagatcgagc cggacatgct gctcgagcac ctcgcacagg
    18901 aaggtctgtc caaatactac atgcccgaat atttcctttg catggaatcg tttccgctga
    18961 cggcgagcgg caaaatcctg aagcgaaggc ttgtcgagat ggtccgtaaa ggcgagctgt
    19021 cgcccattcc gatccgttac cgctcacgcg aggagagccg cgtatgagtg tcaagctgtc
    19081 gaaacgcgat gaattcgcca ttttgacact ggaccggccc gaggcgctca atgcgctttc
    19141 gtttgcgatc atcgacgaga tcgcgaacgc aatcgacgag gctgcgcagt tgccggttcg
    19201 ggcccttatc attaccgggg caggcgaaaa agcgttctgc gccggtgccg acatcaagga
    19261 gctgcgggga agggatctcg tggcccagag acgcggcgcc gagaaggggc aggctgtctt
    19321 cgcgaagctt gaccggcatc ccgttccgtc cgtggccgcc atcaacggat atgccttcgg
    19381 aggcggtctg gagctggcga tggcctgcac attccgtgtc gcaagtccga ccgccaaaat
    19441 gggcttgcct gaaatcaagc tcggcttgat cccagggtat ggcgggacgc aacgccttcc
    19501 acgcctcatc ggcgaggcgc gcgcgctgga gatgatcatg acgggcagga ccgtcggggc
    19561 cgaggaggcg gaacgcatcg gcctcgtcaa tcgcatcgtc gagggcgaca tacttgaggg
    19621 cgcgatcgcg tttgcgcgga gcttcacctg cttcagtctt ccggtgctcg gcctcgcgcg
    19681 cgatgccgtg cgtcgcgctc tcgacctgcc cattcacgaa ggcctgcgaa tcgaggccga
    19741 cctttcgacg cttgcctttc gaaccgccga cgccgaggag ggcatggcgg cctttgtcga
    19801 aaagcgcaag gcggagttca aggatgcctg aaggacagat catcgtcact ggtgcgagcc
    19861 gcggtatcgg agcggccatt gcagccgaat tggcctcgcg cgggtttacg gtcgcggcgc
    19921 tctcccgctc cggagcagcg gcccacggct acggcctggt ctgcgacatg accgatgagg
    19981 ctgccgtcgc gcgtacgatc cggagtattg cggagcgtgg cccgatcgtg ggactggtga
    20041 acaacgccgg ggcgcaacgc gcggagccga gtgccacgct ctctgctgcc gagttcgaat
    20101 cgacaatgcg gctcaacgcg acatcggttc tggtggcgag ccgggaagca tatccacacc
    20161 tcaaggcggc cggtggcggt ctgatcgtga atatgggatc tttcttcgac aaattgggtg
    20221 tcgcgcggaa tctcgcctat tgtgcctcga aggcggcgat cggcgccatc acccgctgcc
    20281 tcgcggtgga atgggcgcgt gacaacattt ccgtcatcga catcgcgccg ggctacatcg
    20341 aaacggatct caatcgcgat tttctcgcga gcgacaaaat caggtcctgg ctcgcccgac
    20401 gcatacccgt cggacggccc gggcagccga acgaggtcgc ccgtctcgtc ggtgttctgt
    20461 tcgccgagcg gatcgacttt ttgaccggcg aaacgatcta tatcgacggt ggccacggga
    20521 tgaaccactg atgaaagtgt tcgagcagct cgagcgccgt cgccgctgga acgaggagga
    20581 ggcgctcatt ctcgagcagg tccgccgtgt ggcagacacg gtgatcgcgc cgaatgcgga
    20641 gcactacgat cgcacgggcg agtttccgtg gaagaacatc gaagctctca acgcgcttgg
    20701 attgaatggc ctgttcgtgc cggaggcata tggcggcgcg ccgatgtcct atcggcttta
    20761 ccttgccgtc gtcaaaattc tcgccgaggc ctgtgcgtcg accggcatca tctatgcgac
    20821 gaacttccat ggaatgaagc cgctgatcga tctcggtacg gcggagcaga aggcgcgtct
    20881 tctcccgcgc attgcctcag gcgggctcgg tgcgctcgca ataaccgagc cgaacgccgg
    20941 atcggacgcc gccggcatgc agacgcgctt tacgccggac ggggacgaga tcatcgtcga
    21001 cggcggaaaa acgttcataa ccaatggcga cgtcgccgat ctgatcctgc ttttcgggaa
    21061 atggagcgaa atcgaggatc ccaggcgcgc catttccgtc ctgatcctgg aaaagggtac
    21121 gcccggcctg gaggtcgtca ggctggagga caaaatgggc cacagggcct cctccacggc
    21181 gacactctcg ttccgggcct gccgtgttcc gcgcgcgaac ctcattgggc agcccggtga
    21241 aggtctgcgc attctgctgt cttcgctcaa caaatcgcgt ccgagcatcg ccgcacacgc
    21301 gctcggcatt gctcacgccg ctttcaagga tatggtgggc tatatgaacg agcgccgcca
    21361 gtccggtcag cgcatcatcg actttcaggg caaccagttt tctctggctg atatggcgac
    21421 cgacctcgcc atggccgagg tgtggctcga ttacgtagcg gggctcctcg aggacgggga
    21481 aacggatttc ggcctcgaag cgtcaatggc gaaaatgcgc gcctccgacc ttgccatgcg
    21541 gattgcgaca gaggcggtcc agatgcacgg cgggtatgga tattgccgtg attatcgcgt
    21601 cgagaggctc atgcgcgatg ccaagatcac ccagatctgg gaaggcacga accagattca
    21661 tcgccaactg atcggacgga gctttgcagc cagatgacga cggaaatccg gacggtcggc
    21721 gtgtgcggcg ccggcggcac gatgggcgca ggcattgcca tcgtggcggc acgcggcggt
    21781 tttcgcacca tcagctacga tcgggatgcg ggcgcgctcg atcgggctgc ggaacagacc
    21841 gcggcgttct tcggcaaatc ggttgaacgg ggcaagctgt cggccgagga tcgcgattcc
    21901 atcctggccc gcatgcatcg caccacgcgc atcgaggacc tcgctgcctg cgatctcgtc
    21961 atcgaagcgg tcttcgagga ccttgaaatc aagcgcacgc tgttcggcaa attggacggc
    22021 atctgtcctg agcatacgat ttttgcctcg aacacatcga ccctgtcgat cactgagatc
    22081 gcttcgggct gctcgcggcc agctaaggtc gtcggaatgc atttctgcct gccggcgcag
    22141 ctcatgaagc tgatcgaaat gtcaccgggt ttgatgacgt cggaagaaac tttcaaggcg
    22201 gcctgggaat ggactgaaaa atgcggtcag atccctgtga aaacgcagga caagcctggc
    22261 ttcattctga acgcgctgct ggttccgttc aacaacgacg caatccgcgc ggtcgaggca
    22321 ggcgtcgcgt caccggctga catcgacaag gcgatcaagt cggcccttgg ctacaagatg
    22381 gggccgctcg agctcgttga tctggtcgga ctcgacacgc agctgcggct ctgcgaggca
    22441 ttttacccca tcacgctcga tccccgcgct gcggcgccgc ctttattgcg gcgaatggtt
    22501 gctgccggcc tcctcggccg caaaaccggt cgcgggttct tcacctacga tggcgcaaag
    22561 atgttcggag cctgacgaca tgcgctatgc aatcatcaac ggcggagtaa gccgatcatt
    22621 cgccgaaaac gatttgcttt tctcatcggc cgccgcagcg gaagatgctg acgttctgat
    22681 ttatgcagac ggcaccaaat tggctcccga tccggccaaa gtggcagtcc ttgtcgagct
    22741 cggaaccgaa agcctggcca tttacaccgg ggaggagcca aatacgtcgt ttgccaacgt
    22801 ggttggctat gcccgctatc ggaacgggga tgatgcgccc tccaagctcg tggagctggt
    22861 gcggcgaccc tggacgaatg acagcgcgct gcaggcggca cgcgatgtct tcgagggcgc
    22921 gggtctggac gtggtggtgt gtgcagacca gcccggtcgg attatcgaca gactggttcg
    22981 cccgaaatac aacgcagcgc tgcgcctgct cgacgaggga ctggccactg aggccgacat
    23041 ggacctgacg tgccggctcg ggctcggtta ccctgatggg ccaatcgagc ggatcaagcg
    23101 tggtggtctg ctccaccact acaagatcac ccaggctctc ttcgagacct acggcacccc
    23161 cgcgtatgcg ccgccgcgcc aggccatcga tgccgcccgt cgagccgggg tgaagaaatg
    23221 aggccgcttg aaggtttgct cgttctcgat ttctcgacct tgttgccagg acccatggca
    23281 accttgcttc ttgccgaatc cggggccgag gtgatcaaga tcgagcggcc gggttccggc
    23341 gaggatatgc gtcattataa accgcgttgg ggccgcgaca gcgtcaactt cgctctgctc
    23401 aatcgcggta agaagagtat tgctctggat ctcaagaatt ccgagcagcg tgcaaagctc
    23461 gatccgctga tcgcgcgggc cgacgttctc gtcgagcagt ttcgcccagg cgtgatggcg
    23521 cgtctgggcc ttgattatga aacgctcgaa aaagtcaatc cgcgcctgat ctattgttcg
    23581 atcacgggtt acggtcagaa tggtccgaag cgcgacgtcg cggggcacga cctcaattac
    23641 atcggcgata cggggctgct ttcgctttcg atgggtccgc tggattcacc tgtcgtgccg
    23701 ccagccctga tcgccgatat cgcgggagga gcttatccgg ccgtcatgaa catcctgctc
    23761 gccctgcagc agcgggcgcg gaccgggcgc ggcagctatc tcgacgtagc catgtccgac
    23821 aatctgtttc cgttcatgta ctgggctatt ggcaacggat tggctgccgg cacgtggccc
    23881 ggcaatggca ccgatctcgt caccggcggc agcccccgct accggctcta tccgaccgca
    23941 gaccggcgca tcgtggctgc cgcgccgctc gagcagaaat tctgggagac gttctgcgaa
    24001 atcataggcc tcgacgccga gctgcgagac gacgcgaaga atcctacggc cacgatcgcg
    24061 cgcatcaccg agctgatcgc ggcagagccc gcaaaaatct gggctgagcg ctttgcgggc
    24121 cgcgattgtt gttgctccgt cgtggcgacc gtctccgagg cgctggaaga tgcgcatttc
    24181 agggcgcgcg ggctcttcag ccatgttctt gccggtgagg gcgggcaaag gctaccggct
    24241 ttgcccgttc cgatcgcgtc catcttccgc ggtgctccgg gtgtacccgt ccctgcgccc
    24301 ggtctcggcg ccaataatga ggactacgaa tgaaagccgt tctcgttcgc gattttgcgc
    24361 cgttcaccca ggtcggtgtc gctgaggtgg acgatcctgt gccggcgccc ggcgaggtca
    24421 tcgtcgatgt cgttgcggcc gaggcgaact atccggatat cctggtcatc gagggcaaat
    24481 accaggtcaa gccgcctctg ccgttttcgc ctggcaaggc ggcggcgggg cgtgtggcag
    24541 ctctcggcga aggcgtccgc aatttttcga ttggcgatcc cgttgccgtg caggtcgagt
    24601 atggagccta tgccgaaaag gtgcgggcgc gagctgaaag ttgctttcga atacccgacg
    24661 gaatgccgtt cgagaaggcg gccgcgctgg gtctggtcta tcaaacggct tggttcgcgc
    24721 tcaaggatcg ggcgagcttc agaaccggcg aaagcgtgct tgtgcttggt gcctcaggcg
    24781 ggatcggggt tgcgagcgcg cagctggcaa aagcgttcgg tgcgggggcg gtcatcgctg
    24841 gcgtcctcgg cgaaagcaac gccgaagtgg cacggagggc cggtgcagac cacgtcgtcg
    24901 acctgggcag ggccgatctc aaggacagcc tgaaagctga ggtcgccgcc gtcaccgatg
    24961 ggcgcggggc agatgttgtc atcgatcccg tcggcggtgc ggcaaacgca gcggctttac
    25021 gcgccatggc ctggaaaggg aggttggttg tcatcgggtt cgcaagcggc gagatcccga
    25081 ccatccgggc aaactatctg cttgtcaaaa acatctcagt ggcagggctg cagtggtccg
    25141 attaccgcga tcggacgccg gagctcgtcg tcgcggctca gaaggaaata tttgatctct
    25201 atacccgtgg cttgatcgat cccgtcatca gccggaccgt gccgctcgag gatttcaaga
    25261 cggccttggc cctgcttcgg gacggtcagg cgcaaggcaa gatcatcctg caggtgcgcc
    25321 aggcttagct accaggcgcc ccaaggttcg caggatcgtc catgcgttta tgggcgggcg
    25381 ccagccaagt gctcactcct gagataattt gcctggccag catcgagatt tgacaaacgc
    25441 cgtcagcttg tgtttgtggg gccgctgtgc tgccggaggc gcctcgggat gtctgccatc
    25501 atcggaacca gcttctgtct aatgaaatga acagcgcgag catagttaca tgcggtgtct
    25561 gcggttcggt gtacgagcgc cggaccgttt ggatttcatc gcgcgatgca ggcgagaagc
    25621 gttgtgaggt ctgcggggag ctgctggaag cgtggaatgg tcccaagctg ccgattttca
    25681 agctggtgaa gcgaggcaat cgccaccagt gaggtcgaca gccagccgcc tgcaccccgc
    25741 tcggctcgtg agttggcggt gcggtaggaa gctcacatcg tcattcacgc cgggagaacg
    25801 gccacgatct cgagagtatc aggatccact tcgacgatct ggttgccggc cagaaagcag
    25861 tgccggtagc ggccgcattt caacctggcg aggtggtcgc gtaacaacgg ccatcgacat
    25921 acccaaatcc tcacgtgagg accatgcggg cgaacattgc tgggctggcg cctgggatag
    25981 ttctgagcga acgcaggctc tcacccgcgc cctcaatccc ttcttgtttt actcttgcct
    26041 gccggccttc cttcgctctc cctccctctc cggttcgtct ctgagccttg ccttcaggtg
    26101 cctgctcgcc gcgtcctgac gggaagagat ggagcgatcg ggcagctaag gcgggaggtt
    26161 gcgcaaggtt cgggaagcta tctccgggag ccgcgtggcc gagctcaagg agtcacgagg
    26221 ggaacagcca ggttttctct gctgtgggat agcgaggaga gacgctccag tcacaagagg
    26281 ctcccatgct caagatcctg accgccacga tgctagccgc gctactgacg ggtacattgc
    26341 tcaccgctgc cgcgattcaa gccgctgaaa cgcgtgctca gcaaacaatt caacgctagc
    26401 cgggcagact tctcgcttcc tgctaccagt gcttgcgctg agccaggaat gattgcgctc
    26461 tagcctcaac ggaatcgaga ccagaactcg gagctcctgc ttccgagcga tcggagccgg
    26521 tgtatgcgcg ctttccggaa taaaatctaa accatctgac aagaatggat ccccttccac
    26581 gacagggaca tcctagtcac cgcctgcggc cgcatctgca tgcaccgcaa gaagatcaac
    26641 atctcgaccg tgctcgccgg tcagaggctc ggaatcaggg aggtcgacga ttgcatttgg
    26701 ctcgtctcgt tcctcgacta tgatctcggc ttcttcgatc gacaggaaga ccgcgtcgag
    26761 cccggaccga acccatttgc cccggagaaa gtgtcaacca tctctccgga ataaaatgta
    26821 accattcgac gagaatggac cctttggtag gtggcgcacc gggcaggatt cgaacccacg
    26881 acccccagat tcgtagactg atccccgcgg gcaccgactg ggactgctta ggatgtgctg
    26941 acacgagaat attacataaa tcagatagtt gaagcgttgc tctcgtctca atgaatttcg
    27001 tgacatccct tgaactcccg tccgcgtggg ccaaatatgg gccaaatcag acggggagac
    27061 tccatggcaa agcaagcagc tcacaacagg attttgccag cc
//
LOCUS       DASVDU010000033         9189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1688375, whole genome shotgun sequence.
ACCESSION   DASVDU010000033 DASVDU010000000
VERSION     DASVDU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9189
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1688375"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF226_02400"
     CDS             complement(<1..424)
                     /locus_tag="VF226_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7072864.1"
                     /translation="MLETFSLQGKATLVTGASSGLGRHFAKVLARAGARVVAAARRID
                     RLEALVAEIKAEGGEATAVALDVTRADSVKGAFDKAEATFGPVTVLINNAGVPSNTFF
                     TKLTENDWRQVMDVNLDGVFRVGQEAARRMIAAGTGGSI"
     gene            complement(545..1522)
                     /locus_tag="VF226_02405"
     CDS             complement(545..1522)
                     /locus_tag="VF226_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7072865.1"
                     /translation="MPTTLWSRRGILGLAASAALALPSVAAAQDYPSKPITFVVPFAA
                     GGPTDTVARLLGEHMGRTLGQQIVIENVGGAGGTVGSERVAKAQPDGYTLLLHHQGIT
                     AAPALYDNLRYDTKTAFEMVGLINHGPMVVVGKNGLEPKDAKSLFEWMKQNADKITMA
                     HAGVGSNSHVCGLIIQKVLGKKFTFVAYRGTGPAMNDLVAGQIDVLCDQSTNAVPQIQ
                     GGTVKGYAVTDTARIDSIKDVPTTPEVGYPDMTMRLWNAIYAPKGTPKEVIDKVNAAL
                     RKALQDPAIVEKLKTFGTTVFPEDQWTPEAHEKAFMAELERQAQLLADK"
     gene            1696..2037
                     /locus_tag="VF226_02410"
     CDS             1696..2037
                     /locus_tag="VF226_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012178707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX7072866.1"
                     /translation="MTIWRPRRWPPLIWGRTVSKIPHFLVHSPKDNVGVVVVEDLKAG
                     TNMLGVITENDTTTELVAKHDIPIGHKVALKPLKAGDTVIKYGEDIGRMVSDAAPGEH
                     VHVHNLKTKRW"
     gene            2058..3248
                     /locus_tag="VF226_02415"
     CDS             2058..3248
                     /locus_tag="VF226_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEX7072867.1"
                     /translation="MADKQNGGSNLTFWGWRRENGRVGVRNHVVILPLDDLSNAACEA
                     VANNIKGTLALPHSYGRLQFGADLDLHFRTLIGIGSNPNVAAVVVIGIEEGWTNKVVE
                     GIAKTGKPVAGFGIELHGDINTIARASYEAKKMLQWASELKREECPISDLWVSTKCGE
                     SDTTTGLGSCPTVGNMYDKLIPQGITGVFGETTEITGAEHICKARAATPEIGEKFYRV
                     WKAYQDEVIEPYKTNDLSDSQPTKGNIAGGLTTIEEKALGNLEKIGRTCRYIDVLEPA
                     ETPSKGPGLYYMDTSSAAAECVTLMAAAGFAVHTFPTGQGNVIGNPIVPVIKISANPR
                     TVRTMSEHIDLDVSGILRREQTIDAAGDALIDMIIRTANGRITSAEALGHREFVMTKL
                     YRSA"
     gene            3500..4672
                     /locus_tag="VF226_02420"
     CDS             3500..4672
                     /locus_tag="VF226_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX7072868.1"
                     /translation="MRITRIRDATAPIASEIRNAYISFTAMTVSLVAVETDVIRDGKP
                     VIGYGFLSNGRYAQGGILRERFIPRLMEADPSSLIDRERDNLDPFAIWRTFMANEKPG
                     GHGDRAHAAGALDMAVWDAVAKIAGRPLWRLLSDRFNGGRFDEKVLVYPGGGYYYPGK
                     ELEGLKAEMCGYQEQGYRVVKMKIGGADLALDLKRIEAVLEVVGRGENLAVDANGRFD
                     LDTALAYGHAMEPYGLFWYEEPGDPLDYQLNAVLAEHYRGAIATGENLFSSIDARNLL
                     RYGGLRPDRDWIQLDPALSYGLTEYLRFLEVVDRMGWSRRRLIPHGGHQLALNMAAGL
                     QLGGSESYPGVFQPYGGFADDIPIVDGHVRLPDVPGIGMELKPAMFSDMQRRLELD"
     gene            4676..5617
                     /locus_tag="VF226_02425"
     CDS             4676..5617
                     /locus_tag="VF226_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX7072869.1"
                     /translation="MAEIVVTEFLDPGPIEILKQKYSVHIDTGLWSNRAELEAQAAEA
                     ICLIVRNRTKVDAALLEKAPKLKVIGRLGVGLDNIDVAACKARGVTVYPALGANAVSV
                     AEYVIATALIFLRGQAYFASSRIAAGEWPREEMSRGHEAAGCRFGIIGYGSIGQIVGV
                     RARCLGMHVVAHDDFLSAENPAWEHAERATLDELLDTCGVVSLHCPLTPETRGLIGAR
                     ELARMKKGAILINTARGGIVDEQALANALRSGHLGGAAVDVFTTEPIDEATAAIFRGV
                     PNLILTPHIAGVTVECNARISAVTVDNVMRALKAAGL"
     gene            5614..6624
                     /locus_tag="VF226_02430"
     CDS             5614..6624
                     /locus_tag="VF226_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX7072870.1"
                     /translation="MSGTVTITQDELERIIAAALVASNTSAHNARSVAVALAQAEVDG
                     QKGHGLSRVPSYTAQAKSGKVDGHAVPEVRQTRPGSLVIDARHGFAYPALDEAIARLP
                     ELARTCGIAAAGITRSHHFGVAGRTVERLAEEGVVALVFGNTPHAMAPWGGRRPVFGT
                     NPIAFAAPQRGEPPVVVDLALSQVARGKIMVAAQKGETIPAGWALDANGEPTTDAKAA
                     LKGTLMPLGGAKGAALAFMVEVLAVALTGANFAFEATSFFDDKGPPPGVGQFLLAIDP
                     AAFAGSEAFLDRFAVLASAIGQDPGARLPGSRRLALRKAAARNGVAVDSKLLDEVRAL
                     AS"
     gene            complement(6695..8491)
                     /locus_tag="VF226_02435"
     CDS             complement(6695..8491)
                     /locus_tag="VF226_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEX7072871.1"
                     /translation="MDGSFLQQAAVFLAAAAIAAPVGRWLKLGSVIGYLAAGILIGPY
                     GLHYVSSVYDVESVRNIAELGVALLLFVIGLELRPVRIWAMRRSVFGAGGAQLILTAL
                     ALACIGMLDGLGTMAAVVIGLGLAMSSTAFVLQGLEERQELTLRHGRMAFSITLMQDL
                     VAIPLLAIIPLLGTHHVSSTTEILIDIGRALGIIAAILLVGRWLLNLIYYLVAKSGVR
                     EALTATGLLIVVGVALLMQAAGLSPALGAFIAGVLLADSAYRHQIEADVAPFEGLLLG
                     LFFTAVGMSLNLNLLERELPLILALAIGLLIVKAIVLYGVGLWHSLDRRGARRLAVYA
                     SQGGEFAFVVFSLAAGAAVIGQELAERLAVVVTLTMAATPLLLLGEGWITRLFRAPEP
                     SYEVPPARDRHVVIAGFGRFGQIIARVLRAKRIPFTALDISPDQITLVQRYGNNQAHY
                     GDASRLDILEAANVGKARAFVLAIDDMDASLKTAAVVRSKFPNVPIYARARNRQHVFR
                     LMDFGITEIRRETFLSAIELTRDVLRGLGLKEPEVRRIIDAFITSDRKRLFEGYEDSS
                     DIEKLQLQARQAAAELETILAEDEEAMRNEPQ"
     gene            complement(8679..9011)
                     /locus_tag="VF226_02440"
     CDS             complement(8679..9011)
                     /locus_tag="VF226_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEX7072872.1"
                     /translation="MKLPALPFTVTNWDSIEPTVHPGETGEARWRTFTVGDLRVRIVE
                     YSAGYLADHWCDRGHVLYVLEGELKTELRDGRTFTLKPGMSYQVSDHGDAAHRSSTRT
                     GAKLFIVD"
     gene            complement(9050..>9189)
                     /locus_tag="VF226_02445"
     CDS             complement(9050..>9189)
                     /locus_tag="VF226_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7072873.1"
                     /translation="HGIGQGKMEYLKSELGPGLDIMRQIKRALDPLNIMNPGKIVALT
                     E"
BASE COUNT         1712 a         2931 c         2947 g         1599 t
ORIGIN      
        1 cgatggatcc gcccgtaccg gcagcaatca tccgccgggc cgcttcctgg ccaacgcgaa
       61 acactccgtc caggttgaca tccatgacct ggcgccagtc gttttcggtg agcttggtga
      121 aaaaggtgtt ggagggcacg ccggcattgt tgatgagcac cgtcactggc ccaaacgtcg
      181 cctcggcctt atcgaatgcc cccttcacgc tatcggcccg ggtgacgtcg agggcaaccg
      241 cggtcgcctc gccgccttcc gccttgattt cggccaccag ggcctcgagc cgatcgatcc
      301 gtcgcgccgc tgctacaacg cgggcgcccg cgcgcgcgag caccttggcg aaatgccggc
      361 ccaagccgga cgacgctccg gtcaccagcg tcgctttgcc ttgcaatgaa aacgtctcga
      421 gcattagacc ctgaacaccc cgttacgcgt gaaatcggca tgtcgcgcct tcggcacccg
      481 cgaaacggat atcccgcgcg tggcgccgaa ggcgctatac catgggcctg gggcctgaaa
      541 cgagctactt gtccgcgagg agctgcgcct gccgctcgag ctcggccatg aaggccttct
      601 cgtgcgcttc cggcgtccac tggtcctctg ggaacacggt cgtgccgaag gtctttaatt
      661 tctccacgat tgccggatcc tgcagcgcct tcctcagcgc ggcgttgacc ttgtcgatca
      721 cttccttggg cgtgcccttg ggcgcgtaga tggcattcca gagccgcatg gtcatgtccg
      781 ggtagccgac ttcgggcgtc gtcggaacat ccttgatgga atcgatccgc gcggtgtcag
      841 tgacggcata gcctttgacg gtcccgccct ggatctgcgg cacggcattg gtcgactggt
      901 cgcagagcac atcgatctgt cccgcgacca ggtcgttcat ggccgggccc gtgccgcgat
      961 aggccacgaa cgtgaacttc ttgcccagga ccttctggat gatgaggcca cacacgtgcg
     1021 agttcgatcc gacgccggcg tgggccatcg tgatcttgtc cgcgttctgc ttcatccact
     1081 cgaacaggct cttggcgtcc ttgggctcga ggccattctt gcccaccacg accatcggcc
     1141 cgtgattgat gaggccaacc atctcgaacg cggtctttgt atcatagcgc agattgtcat
     1201 acagcgccgg cgccgccgtg atgccctggt ggtgaagaag cagcgtgtag ccatccggct
     1261 gcgccttggc cacgcgctcg gatccgaccg tgccgcccgc accgccgacg ttctcgatga
     1321 cgatctgctg gccgagcgtg cgccccatgt gctctccgag caaacgtgcg accgtgtcgg
     1381 tcggcccgcc cgcggcgaat ggaacaacga acgtgattgg tttcgatgga tagtcctgag
     1441 cagcagcaac gcttggcagg gcaagagccg ccgaggccgc gaggcccaga attccgcgcc
     1501 tgctccagag tgtcgtgggc atgtgtttcc tccagcttga tttatcgggt tttcgatcgt
     1561 gagcccctcc cgtccacgac gataaagcgt gttggcgcaa ccgcaagggc cagagcgcaa
     1621 agggggagtt tccgcccgca gtccctcgat cgcctcaact ccgtttacgt gaggttgcat
     1681 aagttataag accgaatgac catctggcgg ccacggagat ggccgccact catctgggga
     1741 agaacagtgt cgaaaattcc acattttctc gtacacagcc caaaggacaa cgtcggcgtc
     1801 gtcgttgtcg aggacttgaa ggccggcacc aacatgctgg gcgtcatcac tgagaacgat
     1861 acgacgaccg agctcgtcgc caagcacgac attccgatcg gtcacaaggt cgctctgaag
     1921 ccgctgaagg ccggcgacac cgtcatcaag tatggcgagg acatcggccg catggtcagc
     1981 gacgccgccc ctggcgagca tgtccacgtg cacaacctca agacgaagcg ctggtaagcc
     2041 cgcgaaagga ctgcatcatg gctgacaaac agaatggcgg gtcaaatctc actttttggg
     2101 gctggcgccg cgagaacggc cgggtcggcg tgcgcaatca cgtcgtcatc ctgccgctcg
     2161 acgatctgtc gaacgccgcc tgcgaggcgg tcgccaacaa catcaagggc accctggcgc
     2221 tgccgcattc ctacggccgc ctgcagttcg gcgccgacct cgacctgcat ttccgcacgc
     2281 tgatcggcat tggcagcaac cccaatgtcg cggccgtcgt ggtgatcggc atcgaggagg
     2341 gttggaccaa caaggtcgtc gaaggcatcg ccaagacggg caagccggtc gccggcttcg
     2401 gcatcgaatt gcacggcgac atcaatacga tcgcccgcgc ctcctacgag gccaagaaga
     2461 tgctgcagtg ggcctccgag ctgaagcgag aggagtgccc catctccgac ctgtgggtct
     2521 cgaccaaatg cggcgagagc gacacgacca cggggctcgg ctcctgcccg accgtcggca
     2581 acatgtacga caagctcatc ccgcagggga tcaccggcgt cttcggcgag acgaccgaga
     2641 tcaccggcgc cgagcatatc tgcaaggcgc gcgccgcaac ccctgaaatc ggggagaagt
     2701 tctaccgtgt ctggaaagcc tatcaggacg aggtgatcga gccttacaag accaacgacc
     2761 tttccgacag ccagccgacg aagggcaaca tcgccggggg actgaccacg atcgaggaga
     2821 aggcgctcgg caatttggag aagatcggcc gcacctgccg ctatatcgat gtgctggagc
     2881 cggctgagac gccgtcgaaa gggccgggcc tctactacat ggacacgtcg tctgcggcgg
     2941 ctgagtgcgt gacgctgatg gcggccgccg gctttgccgt tcacaccttc cccacggggc
     3001 agggcaacgt catcggcaac ccgatcgtgc cggtcatcaa gatttccgcg aacccgcgga
     3061 ccgtgcgcac gatgagcgag catatcgatc tcgacgtctc gggcatcctg cgccgcgagc
     3121 agacgatcga cgccgcgggc gacgcgctga tcgacatgat catccgcacc gccaatggcc
     3181 gcatcacctc cgccgaggcg ctcggccacc gcgagttcgt gatgacgaag ctctatcgga
     3241 gtgcgtgacg cagggacggt gccggaatcg tgttcatttc cggcaccggc tgacactact
     3301 aatcgtccag attgcggaaa gatggatccc ggcgacaagc gccgggatga caacggggtt
     3361 gggctggccc gctccaccac tgcaaagtca tcccgggcct cgtgcccggg atccacgctt
     3421 ccgaggatcg cgcgcctcga atctggatga atccagtgca gcaaagggtt tcgcccgaac
     3481 ggagtggtca accagccaaa tgagaatcac ccgcatccgt gacgccaccg cgccgatcgc
     3541 gtcggaaatc cgcaacgcct acatctcctt cacggccatg acggtgagcc tcgttgcggt
     3601 cgagacggac gtgatccgcg atggcaagcc ggtcatcggc tacggcttcc tgtcgaatgg
     3661 ccgctacgcg cagggcggca tactgcgcga acgattcatc ccgcggctga tggaggccga
     3721 tcccagttcg ctgatcgatc gcgagcggga taatctcgac ccgttcgcca tctggcgaac
     3781 cttcatggcc aatgagaaac cgggcgggca cggcgaccgg gcgcacgccg ccggcgccct
     3841 cgacatggcg gtctgggatg cagtggcgaa aatcgccggg cggccgctct ggcgcctgct
     3901 ttccgacagg ttcaacggtg gccggttcga tgaaaaggtg ctcgtgtatc cgggtggcgg
     3961 ctactactac cccggcaagg agctcgaagg cctcaaggcc gagatgtgcg gctaccagga
     4021 gcagggctac cgcgtcgtca agatgaagat cggcggcgct gatctcgccc tggatttgaa
     4081 gcgcatcgag gcagtgctgg aggttgtcgg acggggcgag aacttggcgg tggacgccaa
     4141 cggccgtttc gacctcgaca ccgcgctcgc ctacggccac gcgatggagc cttacggcct
     4201 gttctggtac gaggagcccg gcgacccgct cgattatcag ctcaacgccg tgctggcgga
     4261 gcattaccgg ggtgcaatcg ccacgggcga aaacctcttt tcgagcatcg atgcgcgcaa
     4321 tctgctgcga tatggcgggt tgaggcccga tcgcgactgg atccagctcg acccggcgct
     4381 ctcctatggc ctgacggagt acctgcgctt tctcgaggtg gtggaccgga tgggctggtc
     4441 gcggcggcgg ctgatcccgc atggtggcca tcagctcgca ctcaacatgg cggccggcct
     4501 gcagctcggc gggtccgaaa gctaccccgg cgtcttccag ccctacggcg ggtttgcgga
     4561 cgacattccg atcgtggacg gccatgtgcg gctgcccgac gtgcccggca tcggaatgga
     4621 gctgaagccc gccatgttct ccgacatgca acgccgactg gagctcgact gaaccatggc
     4681 cgagatcgtc gtcacggagt ttctcgatcc ggggccgatc gagattctga agcagaagta
     4741 ttccgtgcac atcgacacgg ggctgtggag caacagggcg gagctcgagg cgcaggccgc
     4801 cgaggcgatc tgcctcatcg ttcgcaatcg cacgaaagtc gatgccgcgc tcctcgagaa
     4861 ggcgccgaag ctgaaggtca tcggccggct cggtgtcggc ctcgacaaca tcgacgtcgc
     4921 ggcctgcaag gcgcggggtg tcaccgtcta ccccgcgctc ggcgccaacg ccgtgtcggt
     4981 tgccgaatac gtcattgcca cggcgctcat ctttctgcga ggacaggctt atttcgcatc
     5041 atcacgcatc gccgccggcg agtggccgcg cgaggagatg agccggggcc acgaggcagc
     5101 cggctgccgg ttcggcatca tcggatacgg ctccatcggc cagatcgtcg gtgtgcgggc
     5161 gcgatgcctc ggcatgcatg tcgttgcgca cgatgatttt ctgtcggccg aaaatcctgc
     5221 ctgggagcat gccgagcgcg cgacactcga cgagctgctc gatacctgcg gtgtggtctc
     5281 gctgcactgt ccgctcacgc ccgagacgcg gggcctgatc ggcgcccgcg agctggcccg
     5341 catgaaaaag ggcgcgattc tgattaatac ggcgcgcggc ggcatcgtcg acgagcaagc
     5401 cctggccaat gcgctgcgct cgggtcacct cggcggagcc gcggtcgacg tgttcacgac
     5461 ggagccgatc gatgaggcga cggccgcgat ttttcgcggt gtgccgaacc tgatcctgac
     5521 gccccacatc gcgggtgtga ccgtcgagtg caacgcgcgc atcagtgccg tcacggtgga
     5581 taacgtcatg cgcgcgctga aggcagccgg gctatgagcg ggacggttac gattacccag
     5641 gatgagctgg aacggatcat agccgccgca ttggtggctt cgaacacgag cgcgcacaac
     5701 gcccgttcgg tggcggttgc gctggcccag gccgaggtcg acgggcagaa gggccacggt
     5761 ctgagccgcg tgcccagcta caccgcgcag gccaaatccg ggaaagtaga tgggcacgcg
     5821 gtgccggagg tccggcaaac ccggccaggc agtctggtta tcgacgcgag gcacgggttt
     5881 gcgtatccgg ccctggacga ggcaatcgct cgcctcccgg aactcgcgag gacctgcggg
     5941 atcgcagccg ccggcatcac gcgatcgcat cacttcggcg tcgccggtcg cacggtggag
     6001 cgcctcgccg aggaaggcgt cgtcgcgctc gtcttcggta atacacccca cgccatggcc
     6061 ccctggggcg gccgccggcc ggtgttcggc acgaacccga tcgcctttgc ggccccgcag
     6121 cggggtgagc cgcctgtcgt cgtcgatctc gcgctctccc aagtcgcccg tggcaagatc
     6181 atggtcgccg cccagaaagg cgagacgatt ccggcgggct gggcactcga cgcaaacggc
     6241 gagccgacca ctgatgccaa ggcggcgctc aagggcacgc tgatgcctct cggcggcgcg
     6301 aagggcgcgg cgctggcctt tatggtggag gtgcttgcag ttgcgcttac cggagcgaat
     6361 ttcgcgttcg aggcgacgtc cttcttcgat gacaaggggc cgccgccggg ggttggccag
     6421 ttccttctcg ccatagaccc ggccgcgttt gctggctccg aggctttcct cgaccgcttc
     6481 gccgtcctgg catcggccat cgggcaagat cccggcgcgc gtctgcccgg cagccgtcgc
     6541 ttggccctgc ggaaggcggc ggcacggaat ggcgtcgcgg tcgattccaa gctgctcgac
     6601 gaggtccgtg cgctcgccag ttagcgtaac tctcgggagc tacggtcttc gacgccctga
     6661 caatgcagca ggagcgggtc atccgggcga gcgtttactg cggttcgttt ctcatggctt
     6721 cttcatcttc cgcgaggatc gtctcgagct ccgcagcggc ctggcgtgct tgcaactgga
     6781 gcttctcgat atcgctcgaa tcctcgtagc cctcgaaaag acgtttgcga tcgctcgtga
     6841 taaacgcgtc gatgatcctg cggacttcgg gttccttcag cccgaggccg cgaagaacat
     6901 cgcgtgtcag ctcgatggcc gacagaaacg tctctcgccg gatttcggta atgccgaagt
     6961 ccatcagccg aaagacgtgc tggcgattgc gggcccgcgc gtagatcgga acattcggaa
     7021 atttgcttcg aacgaccgcg gctgttttga gcgacgcgtc catgtcatcg atcgcgagca
     7081 cgaaggcccg agctttgccg acgtttgccg cttccaggat atcgagacgg gaagcatcgc
     7141 cataatgagc ctggttattg ccgtagcgct gcacgagcgt gatctggtcg gggctgatgt
     7201 cgagggccgt aaacggaatt cgctttgccc ggagcacacg ggcgatgatc tggccgaagc
     7261 gcccgaatcc ggcgatcacg acatgccggt cgcgagctgg cggcacctcg taggaaggct
     7321 caggggcgcg gaaaagacgg gtgatccagc cctctccaag cagcaggagc ggcgtggccg
     7381 ccatcgtcag ggtgacgacg acggccagtc gttcggccag ttcctggccg atgacggccg
     7441 ctccggcggc gagactgaac acgacgaacg cgaattcacc gccttgcgac gcatagaccg
     7501 caagtcttcg ggcgccgcgc cgatcaaggc tatgccagag cccgacgccg tacagcacga
     7561 tggccttcac gatcagcagg ccaatggcca acgccagaat gaggggaagc tcgcgctcga
     7621 gcaggttcag gttgagcgac atgcctaccg ccgtgaagaa caggccgagc agaagcccct
     7681 cgaatggcgc cacatcagct tcgatctggt ggcggtaggc ggagtcggcg agaagtacgc
     7741 cggcaatgaa ggcgccgaga gcgggcgata gcccggccgc ttgcatcagg agcgcgaccc
     7801 cgacgacgat cagcaagcca gtggccgtca gcgcttcgcg cacaccggac ttggcgacca
     7861 gataatagat gagattgagc agccagcggc cgaccagcag gatggctgcg atgatgccga
     7921 gcgctcggcc aatatcaatc aatatttcag tcgtgctgct cacatggtgc gtgcccagca
     7981 atggaatgat ggcgaggagc gggatggcca cgaggtcctg catcagcgtg atggaaaagg
     8041 ccatgcggcc gtgccgcagc gtaagctcct gccgctcctc gagcccctgg aggacgaaag
     8101 ccgttgacga catggcgagc ccgaggccga tcaccacagc agccatcgtg ccgagcccgt
     8161 cgagcattcc aatgcaggcc aacgccagcg cggtcaggat gagttgcgcg ccgcccgccc
     8221 cgaacacact gcgccgcatg gcccagatcc gcaccggccg caactcgaga ccgatcacga
     8281 acagcagcaa ggcgacgcca agctcggcga tattgcgcac gctttcgacg tcgtagacgc
     8341 tactgacgta gtggaggccg tagggaccga taagaatgcc tgcggccaga tatccaatca
     8401 cggagccgag cttgagccag cgcccgacgg gagctgcgat ggccgcagcc gccaaaaaca
     8461 ctgccgcttg ctgcaggaac gagccatcca tcgcggagat ttcccgacaa gagattgcag
     8521 agataccgct caaagcggcc gacgcccgca ggttccgtaa agcgcgcgac gtttggcagt
     8581 aagcaggtct cgccgaattg catgaaggcg aggggtggac cggcactgat caaagccgat
     8641 ccgctgctca attcatcgca ggctggtcgt gggtgctgct agtcgacgat gaaaagcttg
     8701 gcgcctgtgc gggtagatga tcgatgagcg gcatcgccgt ggtctgagac ctgatagctc
     8761 atgccgggct tcaacgtgaa tgtgcgcccg tcgcggagct cggtcttaag ttcgccctcg
     8821 agaacataca gaacgtgacc gcgatcacac caatggtcgg cgaggtagcc tgccgaatac
     8881 tcgacaatcc ggacgcgcag atcgccaacc gtgaacgtgc gccagcgggc ctctccggtt
     8941 tcacccggat gaaccgtcgg ctcaatgcta tcccaattcg ttacggtgaa tggcagtgcc
     9001 gggagcttca ctgtgcccct cctctcgggt tcagcactgg ggtgtcggct cactccgtca
     9061 gcgcgacgat cttccccgga ttcatgatgt tcagcggatc gagcgcacgc ttgatctggc
     9121 gcatgatgtc gaggccgggg ccgagctccg acttcaggta ctccatcttg ccttggccga
     9181 tgccgtgct
//
LOCUS       DASVDU010000034         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_138419, whole genome shotgun sequence.
ACCESSION   DASVDU010000034 DASVDU010000000
VERSION     DASVDU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138419"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF226_02450"
     CDS             complement(<1..293)
                     /locus_tag="VF226_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072874.1"
                     /translation="MTSETKAVVEIVGATKRFGAVTAVDDLSLAIRENEFFALLGPSG
                     CGKTTLLRMIAGFERPDAGRILLDGRDITDLRPNRRPINMMFQSYALFPHLSV"
     gene            complement(290..1072)
                     /locus_tag="VF226_02455"
     CDS             complement(290..1072)
                     /locus_tag="VF226_02455"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072875.1"
                     /translation="MTMRAGRRWLVGHLGLVYLFLYGPILVLVALSFNKSGLPTAWTG
                     FSLEWYGRMLRSQAIIASVGNTLIVAAASTLIATTLGTLLALGLDRLHPGALLEGFVF
                     VPMIIPDIVLAIALLSFFTLIGMTLGLHSIILAHAVFNIAFVCAVVRTRLRHFDRSII
                     EASIDLGASEFTTFRRVTLPAIFPGVLAGALLAFTLSVDEFIIAYFTSGAGRASTTFP
                     MQIYAMIRFGVTPEINAVATIVLGFSFLLILLSQRLNRGATP"
     gene            complement(1133..1966)
                     /locus_tag="VF226_02460"
     CDS             complement(1133..1966)
                     /locus_tag="VF226_02460"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7072876.1"
                     /translation="MLLGPGLGWLALFLLVPCGVVFVYSFFLRGIYGGIDYVFTLENY
                     ARALDPLFLSIFLDSAKIAALATLLALLVGYPAAYLIATGPRRWQVPLLVLVMLPFWS
                     NYLIRTYAWIVLLNREGLVNRALGGLGLIETPLPLLYNEFAIVTGLLYGYLPFMVLAL
                     YSSISRLNPEIREASADLGAPAWRTFLRVTLPLTVPGIATGSVFVFVLSIGNFITPDL
                     LGGGRTVMVGNLIYDQFLSARDWPFGSSLAFLLIGVMMLLLFLQAWAVNRSQAAGGGR
                     D"
     gene            complement(2089..>2486)
                     /locus_tag="VF226_02465"
     CDS             complement(2089..>2486)
                     /locus_tag="VF226_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7072877.1"
                     /translation="VSGEASLVHAWDGWCNYGIAENPDIKFVVPKEGSDMWVDTMVVT
                     AESENKHAAHAFINYVLRPEIHSWVAENILYKVPNEPAMEGLDPDLVKTYPNLGMTPE
                     ELLRMEQLRDLGRAQRDYTRAVTEIMASR"
BASE COUNT          512 a          769 c          856 g          349 t
ORIGIN      
        1 tggaccgaaa ggtgcgggaa cagcgcgtag gactggaaca tcatgttgat cggccgccgg
       61 ttggggcgga gatcggtgat gtcgcggccg tcgagcagga tgcgccccgc atcgggccgc
      121 tcgaacccgg cgatcatccg cagaagcgtc gtcttgccgc agccagaggg accaagcagc
      181 gcgaagaact cgttctcgcg gatcgcgagc gagagatcgt cgaccgcggt caccgccccg
      241 aaccgcttgg tggcacccac gatctccacc accgccttgg tttcgctcgt cacggcgtcg
      301 caccccggtt cagccgttgg gacagcagga tcagcaggaa gctgaatcca agtacgatgg
      361 tggcgacggc attgatctcc ggcgtcacgc cgaagcggat catcgcgtag atctgcatgg
      421 ggaaggtggt cgaggcgcgc cccgcaccgg aggtgaagta ggcgatgatg aactcgtcga
      481 ccgaaagcgt gaaggcgagc agcgcaccgg cgagtacgcc cggaaagatc gccggcagcg
      541 tgacgcgccg gaaggtggtg aattcgctgg ccccgaggtc gatcgaagcc tcgatgatgg
      601 acctgtcgaa atgccggagc cgcgtgcgca cgacggcgca gacgaaggca atgttgaaga
      661 cggcgtgcgc caggatgatc gaatgcaggc cgagggtcat gccgatcagc gtgaagaagg
      721 agagcagcgc gatcgccagc acgatgtcgg gaatgatcat cggcacgaag acgaatccct
      781 cgagcagcgc gcccggatgc agccgatcga gaccgagcgc gagcagcgtg cccagggtgg
      841 tcgcgatcag ggtcgacgcc gcggccacga tcagcgtgtt gccgaccgag gcgatgatcg
      901 cctggctacg cagcatccgg ccgtaccatt ccagggagaa gccggtccag gccgtgggca
      961 gaccgctctt gttgaacgac agcgcgacca gcaccaggat cggcccgtag agaaacaggt
     1021 agacgagacc aagatggccc acgagccagc ggcgcccggc gcgcatggtc atgctccgcc
     1081 cgcgatgcat cctctcccac aagcggagag gaagcgagcg gatgccgacc ggctagtcac
     1141 ggccgccccc agccgcctgc gaccggttca cggcccaggc ctggaggaac agcagcagca
     1201 tcatgacgcc gatcagcaga aaggcgaggc tggagccgaa cggccagtcg cgggcgctca
     1261 ggaactggtc gtagatcagg ttgccgacca tcaccgtccg gccgccgccg agcaggtccg
     1321 gcgtgatgaa gttgccgatg ctgagcacga agacgaacac cgacccggtg gcgatccccg
     1381 gcacggtgag cggcagggtg acgcgcagga aggttcgcca ggccggcgcg ccgagatcgg
     1441 ccgaagcctc gcggatctcc gggttgaggc gggagatcga gctgtagagg gcaaggacca
     1501 tgaagggcag atagccgtag agcagccccg tgacgatggc gaactcgttg tagagcagcg
     1561 gcagcggcgt ctcgatcagg ccgagcccgc cgagcgcgcg gttgaccaga ccctcccggt
     1621 tcaggagcac gatccaggcg taggtgcgga tgagatagtt gctccagaac ggcagcatga
     1681 cgaggacgag cagcggcacc tgccagcgcc gcggccccgt cgcgatcaga taggcggccg
     1741 ggtagcccac cagaagcgcg agcagcgtgg cgagcgccgc gatcttcgcc gaatcgagaa
     1801 aaatggagag gaacagcggg tcgagcgcgc gggcatagtt ctccagcgtg aagacatagt
     1861 cgatgccgcc atagatgccg cgcaggaaga agctgtagac gaacacgacg ccgcagggga
     1921 cgaggaggaa cagcgcgagc cagcccagcc ccggcccgag cagcaccaga cgctgaacga
     1981 attcgcccga tcgtgtcatg agcattcgag caccacagag gcacagagaa cacagagatc
     2041 caggatttcc tctgcgtctc tgtgcctctg tggttcaagc catgagctct accgcgacgc
     2101 catgatttcg gtgacggcgc gggtgtagtc gcgctgggcc cggccgaggt cgcggagctg
     2161 ctccatccgc aagagctctt cgggcgtcat gcccaggttc ggataggtct tcaccaggtc
     2221 cggatcgagg ccctccatcg ccggctcgtt cggcaccttg tagaggatgt tctccgccac
     2281 ccaggaatgg atctccggcc gcaacacgta gttgatgaag gcatgcgccg cgtgcttgtt
     2341 ctccgactcg gcggtgacca ccatggtgtc gacccacatg tcgctgcctt ccttcggcac
     2401 gacgaacttg atgtccgggt tctcggcgat gccgtagttg caccagccgt cccacgcgtg
     2461 cacgagactg gcctcgccgg agacga
//
LOCUS       DASVDU010000035        12374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1494148, whole genome shotgun sequence.
ACCESSION   DASVDU010000035 DASVDU010000000
VERSION     DASVDU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494148"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF226_02470"
     CDS             <1..210
                     /locus_tag="VF226_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX7072878.1"
                     /translation="LSAAMMLDWLGERHGSDSARAAARAVEKSVDLVFGSGRVRSCDI
                     GGSDGTVAIGRAVADAIRSGEADPA"
     gene            482..1888
                     /locus_tag="VF226_02475"
     CDS             482..1888
                     /locus_tag="VF226_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072879.1"
                     /translation="MLRAEPYVAIAHKPLLASCFALMLLSLTAIAANGSPFDTAQNLD
                     CDAVNNGALNIELGGFTRATRRVTLRAHETLDFALEAGADAFGSLTLVAGKGSPRPLL
                     AGPAGTTVRFVAPARDTFGFEFVAEGEEDVAFTATCSRAQSARRNGLSASQRAAKLLA
                     EKPNLRSLPEPELPASIDPEVWDLLQESQPTAANPSVVRRRPATASLRAIRPPDGLDV
                     WLQADGNRYSLGEQEKAPQESDATDLSGGGLKFKVLPEIMIGALVNFDQQGEQLIKAP
                     LSLSDRGWMAGPMATVQLVPGISLDARAAWGESRPAAPFSAASGLGAERRLVNARLAN
                     TQTFGHWRFSPSVVVNYVEEEQHFAGLSTEGGPARQTFGSGRVDIRPELSYRLDLGDS
                     TFIEPKAAISSFWDIDSLSRLAPGSSEQDMRIKAEAGVTIGTVTGTKLQATGGVEEGG
                     AGAEDIWSGRFQLSVPLK"
     gene            2140..2766
                     /locus_tag="VF226_02480"
     CDS             2140..2766
                     /locus_tag="VF226_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072880.1"
                     /translation="MDFPGFFAAAPTITLHDPLAEFLGASKGGVMTYGYADAVKLAGH
                     SCPTVAGAYLMVRRGLAYLYGDELPERGGIEVYLRDGREQGTTGVIATVAMLITGAAP
                     ETGFGGLGTGGRFSRRGLLQFDTPIDGLLAMRRRDTGRGVVLDLDLAKVPSDPTMGPL
                     FVKAMAGQTDEDEQSRFAELFQGRAARMLLEHANDSNLIQACDWEAVA"
     gene            2784..3221
                     /locus_tag="VF226_02485"
     CDS             2784..3221
                     /locus_tag="VF226_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072881.1"
                     /translation="MSYFALLILHLFAAIMFVGTVFFEVLILEGIRKPVGREIMRKIE
                     LAIGRRARRLMPFVMLVLYGAGISMAWQYRSALSNPFSSTFALMLWIKIALATSVLAH
                     FLTAITLSGTGKLKSRHFQIIHLSVFSHVVLIVFLAKAMFYLG"
     gene            complement(3334..3723)
                     /locus_tag="VF226_02490"
     CDS             complement(3334..3723)
                     /locus_tag="VF226_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX7072882.1"
                     /translation="MAKRIPSENVRLKRAYEPASSSDGMRVLVDRLWPRGVKKADAKI
                     DEWMKDIAPSSELRKWFGHDPARWPEFRKRYAAEIKEHPEQLQRLRALAREGALTLVF
                     SASDEEHNNAVVLRDMILDQSGRHAQK"
     gene            complement(3853..4398)
                     /locus_tag="VF226_02495"
     CDS             complement(3853..4398)
                     /locus_tag="VF226_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7072883.1"
                     /translation="MRVLAAIGVLAILAVIAGAVYFFAGFYNVAANVTEPDIVSSSLV
                     SVRQASVARHAEGITPPVSMDDSSVIQSGARAFAARGCANCHGAPGVQWAKFSEGLEP
                     AVPDLKEVGPPRDPAQIFWIIRNGIRMTAMPSFNRAGVNDDEIWTLTAFVKNLDKISE
                     ADFQAWTAPPAEGGTDGGGPP"
     gene            4844..5314
                     /locus_tag="VF226_02500"
     CDS             4844..5314
                     /locus_tag="VF226_02500"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7072884.1"
                     /translation="MAAVIGCGFLFALALNFANVVGRYVFHAPIYWAEEAMIFTFAWC
                     VFLGAALVALRSNHLRVELLDWVLPEKARRALAILIHLVTIALMIFVAWHAWSLVEMV
                     ERVQQTSIVGEIPMTVPYGAVLVGSTLLAFASLVRTIELITGIEKPSEPGPLPP"
     gene            5311..6588
                     /locus_tag="VF226_02505"
     CDS             5311..6588
                     /locus_tag="VF226_02505"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7072885.1"
                     /translation="MTWLIALLPLLLLALGFPIYVVLLASTSTIAMIYMDIPLTLVPQ
                     VMFGSLDKFTLLAVPFFIFAGEIMSEGGISRRIVEWLLSMVGRLRGGLAYATIGASEF
                     FGAVSGSSPATVAAIGKLMYPALLRGGYSDRFSLGLITSSGAVASVMPPSIVMILYGA
                     ASEQSVAALFAGGVVPALLIGALTVSYIGWASRRQTPSDEHRLALPLVWQATRDAVWA
                     LGTPIVILGGIYLGIFSPTEAAGVASVYGIFVTMAIYREVDIAGLWRITLNSALLTSQ
                     ILIIVAASGVFSWLLTVSGIPQALVQILGGSAQTTWTMLLLVNVFLLIVGCFVDPVSA
                     IIILTPLLVPLMQSLGVDLVHFGIFLTVNLSIGMFTPPFGLNIFVSQALFKAPLPTIY
                     RGLVPFIVLQLIALAVVTYVPEVSLTLADLVRK"
     gene            6693..7709
                     /locus_tag="VF226_02510"
     CDS             6693..7709
                     /locus_tag="VF226_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7072886.1"
                     /translation="MFSLRHYLPLAIATALAGLTGLSAPAKAQTTMKLATATVNDVQV
                     EAIKYFGAKVEARSNGRIKAEYYPGAQLGSNPRMIEGLQLGTIEVYVGPPAYMVGLDS
                     RFQVMDAPGLFDDMEHFYRVTQDKEFRDTFLALGERKGVKGISLFVYSPTSFATRDPI
                     RKLPDFQGKKLRVLASKLERESVGKLGATAVPIDFSEVVSALQQGTVDGMRTGLSVFT
                     SMKVYEVVNYVTTTHEAMIPEVMMVSKAWFDRQPDDIKQIIVEEAAGMERHLFDWTSR
                     QQVEAEKIWKEKGGEIIQLPEADRKEMMQQLAQVAEQVLSEKPTDKQMFEMLKQAVER
                     ARGK"
     gene            7712..8740
                     /locus_tag="VF226_02515"
     CDS             7712..8740
                     /locus_tag="VF226_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7072887.1"
                     /translation="MWKLEPTKVAAIQAAPVFLDSTATTEKACRLIASAAEQGARVIA
                     LPEVFIPGYPYWSWMMNPLEGSKWFRALYKASILVPGPETNLLGRAARDAGAYVVVGV
                     NERSPNSIGTIYNTNLVFGPDGSLLGRHRKLVPTFAEKLTWASGDGSSLRTYETPYGA
                     IGTLACGENTNTLARFTLLSQDERIHIANYIAFPFTENYDMPEAIRIRAGAHAFEGKL
                     YTLVSCSAMSDQIADMLCTTDEQRRMMSGRPNAFSGIFGPDGRLVTDPLIDEEGIVVA
                     EIDPERCIEPKQFQDITGAYNRFDIFDLRVNRRALAPAQFADAAPAERSPQQLEPQSE
                     RMRVSEQD"
     gene            8863..9678
                     /locus_tag="VF226_02520"
     CDS             8863..9678
                     /locus_tag="VF226_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7072888.1"
                     /translation="MGFRLLSALTAARSSLPLCDLARSSGMSPSKAHRYLASFMKTGL
                     VEQDPATRDYRLGPFAFDMGLAAFGSSDHLRHAIDVQIKLRDELDETVVLTVWGSHGP
                     TVLRVEENSDPVIMTMKVGATLPLMQSAAGRIFAAFMPRRIIESYLQVANFSASVYSR
                     REIVRYDDIEPELEEIRRTRLSVSNGEVLRGVNAVAGPLLAPHGQLVGALGVIGQQES
                     LDMSSDGHIARVLHRAVVAYERSAAISAANRPRSLAAPQSDDARHRARPNRER"
     gene            9679..10719
                     /locus_tag="VF226_02525"
     CDS             9679..10719
                     /locus_tag="VF226_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7072889.1"
                     /translation="MPLPLLPTSLVGSYAQPEWLIDRKKLAGRFPPRVRATELWRVAP
                     EFLAEAQDDATLLAIRDQERAGLDIITDGEMRRESYSNRFATVLEGVDIDNPGTALDR
                     SGHPNPVPRVVGKIRRREPVEVRDVEFLRANTDRTIKITVPGPFTMSQQAQNDFYASE
                     EELALDYAGAVNEEIKDLFAAGADIVQIDEPYMQARPEKARQYGLPALARALDGVTGT
                     TALHICFGYAAIIHERPSGYSFLPELAHAPVQQISIETAQSNLDLSVLEKLPGKTIIL
                     GVLDLSDMNVETPETVAARIRRALPHVPAERIVVAPDCGLKYLPREVAFGKMRAMVEG
                     AKIVRQELAGSR"
     gene            complement(10917..11867)
                     /locus_tag="VF226_02530"
     CDS             complement(10917..11867)
                     /locus_tag="VF226_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072890.1"
                     /translation="MKAVRTRFRAYQLGSPGSSFSYFAGGHFTLIEARLTDTNKATII
                     QEMAACGVKAVDTLHITSWDSDHCCASELIDLLALAVPRTIECPGYEPHTDNGRESAR
                     IIKAYKETKRNSNRPVELRTISPEYIKGLQSASQLAFDDVFYHPRFIDPDCTNNNSTV
                     KLFRGGSFNVLSLGDVESHNLSAYLRRRTILQRETDVMILSHHGADNGFTNKPFLSHI
                     RPSLAICSSNYDNQFDHPRDEIRKLLHERDIRLMTTKTGDVVVMSVGDHTGRYRAMNL
                     KADSTEVSSTCDFYSKKANLLQYNDDTLRQLFGRRPSYPR"
     gene            11953..>12374
                     /locus_tag="VF226_02535"
     CDS             11953..>12374
                     /locus_tag="VF226_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7072891.1"
                     /translation="MKIGLALSGGGMRAAAFHCGVLRRLAEQDLLENVAQLSTVSGGS
                     LMVAALLGFAENKWPTSGEFRDRIYSSARTLFTTTDLLSLRAIGLSGAVRFNARLLTH
                     RATVLADMLERRWRVSGMLADLPEQPIWWINATSVETG"
BASE COUNT         2202 a         3903 c         3862 g         2407 t
ORIGIN      
        1 ctctcggcgg cgatgatgct cgactggctc ggcgagcggc acggcagcga tagcgcgcgt
       61 gcggccgccc gcgccgtcga aaaaagcgtc gacctcgttt ttggcagcgg gcgggtccgt
      121 tcctgcgata tcgggggcag cgacggcacc gtcgccatcg gccgcgcggt ggccgacgcg
      181 atccgctctg gggaggccga cccggcatga ccttgtcagt cgccatcgcc ggtctcggct
      241 gcctgcttgg cgacagcatc gccgctctgc gcgtgccggt gccaggtgtc ccgccggtgc
      301 attggtggca gtgaagctgc cggcaaaccg catcgaagcg atccgctgca gcctgcggct
      361 gaccccgccc gctggacggc ggagatccga caggtgggtg aggccgactc acaatgttgt
      421 ccatattgcg ttgcaacaga gcgcttgagt attgcgggga acgaactcga tgggagactt
      481 catgctgcgc gcggagccat acgtcgccat agcccacaaa ccgcttctcg cgtcgtgctt
      541 cgcgttgatg ctcctgtcgc tgaccgcgat cgcggccaac ggcagcccgt ttgacacggc
      601 gcagaacctc gactgcgacg ccgtcaacaa cggtgccttg aacatcgagc tcggcgggtt
      661 cacgcgcgcc acgcggcgtg tcacgctgag agctcacgag acgctcgact ttgccctcga
      721 ggccggcgct gatgccttcg gatctctgac ccttgtcgcg ggaaagggat caccacggcc
      781 gctgcttgct ggacccgccg gcacgacggt caggttcgtc gccccggcgc gagacacctt
      841 tggtttcgag ttcgtcgcgg agggcgagga ggacgtggcc ttcaccgcaa cttgctcacg
      901 tgcgcagagc gcccggagga atggcctgtc ggcatctcag cgggccgcca agcttcttgc
      961 cgaaaagccg aacctccggt ccctgcccga gccagagctc ccggccagca tcgatccgga
     1021 ggtgtgggat ctcttgcagg agagccagcc tacggctgcc aacccgtcag tggttcgacg
     1081 gaggcctgcg accgcgagct tgcgtgcgat ccggccgccc gacggcctgg atgtgtggct
     1141 gcaggcggac ggaaaccgct actcgctcgg cgagcaagag aaggccccac aggagagcga
     1201 tgcgactgat ttgagcggcg gcggcctcaa gttcaaagtg ctgccggaga tcatgatcgg
     1261 tgcactcgtg aatttcgacc agcagggcga acagctcatc aaagcgccgc tcagcctttc
     1321 ggaccgaggg tggatggccg gccccatggc tactgtccaa cttgttcccg gcatctcgct
     1381 cgacgcgcgc gcggcctggg gcgagagccg gcccgccgcg cctttctcgg ccgcaagcgg
     1441 actcggtgcc gaacggcggc tcgtcaatgc ccggctcgcc aatacccaga catttggcca
     1501 ctggcggttc tcccccagcg tggtcgtcaa ctacgtcgag gaagagcagc actttgcagg
     1561 cttatcaacc gagggcgggc ctgcgcggca aacattcggc tccggccgcg tcgatatccg
     1621 gccagagctc agctaccggc tcgatctcgg cgattcgacg ttcatcgagc ccaaggctgc
     1681 catcagcagc ttctgggaca tcgacagcct gtcgaggctc gcgccgggca gctcagagca
     1741 ggatatgcgg atcaaggcgg aggccggtgt caccatcggc acggtcaccg gaaccaagct
     1801 tcaggcgacc ggtggtgtcg aagaaggcgg ggcgggagcc gaggacatct ggagcggacg
     1861 gtttcagctc agcgtgcctc tgaagtgagc tgggctgagt ctgcctgcat ggcgtcaagg
     1921 ttaaggaagg aatccgcacc ctagaaccaa ttttttagaa ccaatttttt ggcgccaggg
     1981 acgaccgccg atagatactt ctcgcacccc atttccgtcg gcacgccgga ctggtgcatc
     2041 gggatgtcgc cgttgccagc tctgaaaaga tgcattatag cgtcatgtta acgcccgcac
     2101 cccgcgggcg ctctgtctga tgtcatccca gaggatgcaa tggacttccc cggtttcttt
     2161 gctgcggcgc cgacgatcac gctgcacgat ccgctcgcgg aattcctggg cgcgtcgaag
     2221 ggcggcgtga tgacctacgg ctatgctgat gccgtgaagc tcgccgggca ctcctgcccg
     2281 accgtcgccg gagcctacct catggtgcgc cgggggctag cctatctcta tggcgacgag
     2341 cttcccgaac gcggaggcat agaggtctat cttagggatg ggcgcgagca agggacgacg
     2401 ggcgtgattg caaccgtcgc gatgctgatc actggcgccg cgccagagac tggctttggc
     2461 ggcctcggga ccggcggccg cttctcgcgc cggggcctgc tccaattcga cacacccatc
     2521 gatggccttc tggcgatgcg gcggcgcgat accggccgcg gtgtggtgct cgatctcgac
     2581 ctggccaaag taccatcgga cccaaccatg gggccgcttt tcgtgaaggc catggccggg
     2641 cagacggatg aagatgagca gtcccgcttc gcagaactct ttcagggtcg tgccgcgcgg
     2701 atgctgctcg agcatgccaa tgactcgaac ttgattcagg cctgcgactg ggaggccgtg
     2761 gcgtaagcgc cagccggacg accgtgagct atttcgcgct cctcatcctg catttgttcg
     2821 ccgcgatcat gttcgtcggc acggtcttct tcgaggtgct gatcctcgaa ggaatccgca
     2881 aaccggtcgg acgcgagatc atgcgcaaga tcgagctcgc catcgggcga cgcgcccggc
     2941 ggctgatgcc cttcgtcatg ctagtgctct atggcgcagg catcagcatg gcctggcagt
     3001 accgcagcgc cctctcgaat ccgttctcga gcactttcgc cttgatgctt tggatcaaga
     3061 tcgcgctcgc aaccagtgtt ctagcccatt tccttaccgc cattacgttg agcggcacag
     3121 gcaagctcaa atcgcgccac ttccaaatca ttcatctcag cgtgttctct cacgtcgtgc
     3181 tgatcgtctt tctggcaaaa gccatgttct acctgggctg acagcgcacg atcgcgacta
     3241 tcgcggcggc catgaattgc gaatgggcgg ccatagcagc ctcacggatg cagtatcggg
     3301 agcactatct ttctgggata tttctgcgct tgactatttt tgggcgtgcc ggccgctttg
     3361 atccagtatc atatcccgca gcacgacggc gttgttgtgc tcctcgtcac tggcagaaaa
     3421 gacgagcgtg agggcccctt cacgcgcgag cgcccgcaac cgctgcagtt gctcagggtg
     3481 ctctttgatc tctgccgcgt aacgcttccg gaattccggc cagcgcgcgg ggtcgtggcc
     3541 aaaccatttc cgcaactctg aactcggggc gatgtccttc atccattcgt cgattttggc
     3601 gtctgctttc ttcacgcccc tcggccacag cctgtcgacc aagacgcgca tgccatcaga
     3661 actgctcgcc ggctcgtagg cccgcttcaa tctcacgttc tcgctaggaa ttcgtttggc
     3721 catcgaaatt ctcctcgtga gtatttttcg gcaagcacct cccgcaacgt gactgcgcct
     3781 agtgcgattg gtcatttttg gttcgagaac gggcctgaga ctggcggctt tcgcaacgtc
     3841 cggccgttat actcacggcg gccctccgcc atccgttccg ccctctgcgg gcggtgctgt
     3901 ccaggcctgg aaatcggcct cggatatctt gtcgaggttc ttcacgaatg cggtgagcgt
     3961 ccagatttca tcatcattca cacccgctct gttgaaggac ggcatcgccg tcatccggat
     4021 gccattcctg atgatccaga aaatctgcgc tggatctcta ggaggcccaa cctccttgag
     4081 atccgggacg gcgggctcca agccctccga aaacttggcc cactgtactc caggtgcacc
     4141 atggcaattc gcgcagcccc tggcagcaaa ggcgcgggcg cccgactgga tgacgctgct
     4201 atcgtccatc gagactggag gcgtgatgcc ttccgcatgc ctcgcgaccg aggcttgtcg
     4261 gaccgatacc aggcttgagg acacgatgtc cggctccgtc acgttggcgg ctacattgta
     4321 aaagccggca aaaaagtaga ctgcgccggc aatgacagcg agaatcgcga ggacaccgat
     4381 tgcggccaga actctcatcg cgtgtatcct cttctcagga gcaaaagccc tgctccgcta
     4441 tcgagcagcc gaagagccag cacccttcat agcacaagat cgggcggcca agcgcgaccc
     4501 gcgcgtagat cggtgaagac actcatagac gatccgaagg caggcacgcg ttcagggccg
     4561 caataaaaat taagtaattc agaacgataa cagattgttg gcgagccggc ttggtaggcg
     4621 gcacctcgac gtcggccgat gacgccgagt tcggccggat taagcatcca agcaattcca
     4681 gtgcctctcc gccggcccga ctcctcgcct ctattcccca ttgtgcaacg ggttcactcg
     4741 atcacgatgg cagcccgccg ttagacgact atagtcgatc tcattctacc tttcgcccgg
     4801 gccgccttgc atgcgcgtcg tcttgaacat ccttgaacat ctgatggccg ccgtcatcgg
     4861 ctgcggcttt ctgttcgcgc tggcgctcaa cttcgccaac gtcgtcgggc gctatgtctt
     4921 ccacgcgccg atctactggg ccgaggaggc gatgatcttc acattcgcct ggtgtgtgtt
     4981 ccttggcgcc gcgctggtcg ccctgcgcag caatcatctg cgcgtcgagc ttctggattg
     5041 ggtgctcccg gagaaggcac ggcgggcgct ggcgattctc atccacctgg tcacgatcgc
     5101 tctgatgatt ttcgttgcgt ggcacgcctg gtccctggtg gagatggtcg agcgggtgca
     5161 gcagaccagc atcgtgggcg agatcccgat gacggtgccc tatggcgccg tcctcgtcgg
     5221 cagcactctg ctcgcgttcg cctcgctggt gcgtactatt gagctgatca ccggcatcga
     5281 gaagccatca gaaccgggcc cgctaccgcc atgacgtggc tgatcgcact cctcccgctg
     5341 ctgctgttgg cgctcgggtt tccgatctac gtggtgctgc tggcgtcgac cagcaccatc
     5401 gcgatgatct acatggacat cccgctcacg ctcgtgccgc aggtgatgtt cggcagcctc
     5461 gacaaattca ccctgctcgc ggtgccgttc ttcatcttcg ccggcgagat catgagcgaa
     5521 ggcggcatct cccgccgcat cgtggagtgg ctgctgagca tggtcggcag gctgcggggc
     5581 gggcttgcct atgccaccat cggtgccagc gagttcttcg gcgcggtgtc gggctcgagc
     5641 ccggcgaccg tggcggcgat cggaaagctg atgtatccgg cgctgctccg tggcggctac
     5701 tccgaccggt tctcgctcgg gctcatcact tcctccggcg cggtcgcaag cgtgatgccg
     5761 ccgagcatcg tcatgatctt gtacggcgct gcctccgagc agtcggtggc ggccctgttt
     5821 gcgggcggcg tggtgccggc gcttctgatc ggcgctctca cagtcagcta tatcgggtgg
     5881 gcatcaaggc ggcagacgcc cagcgacgag caccggctgg ccttgccctt ggtctggcag
     5941 gcgacgcgcg acgcggtttg ggcgctcggc acaccgatcg tcatcctcgg cggcatctat
     6001 ctcggcatct tctcgcccac cgaggcggcc ggagtggcca gcgtctacgg cattttcgtc
     6061 acgatggcga tctaccgtga ggtcgacatt gccggcctct ggcgcatcac cttgaactcg
     6121 gcgctgctga ccagccagat cctgatcatc gtcgcagcgt cgggtgtgtt ctcctggctg
     6181 ttgactgtca gcggtatccc gcaggcgctg gttcaaatcc tgggaggaag cgcgcagacc
     6241 acctggacga tgctgctgct ggtcaacgtt ttcctgctga tagtcggatg ctttgtcgat
     6301 ccggtgtccg ccatcatcat tctaacgccg ctgctggtgc cgctgatgca gagcctcggt
     6361 gtcgatctag tgcatttcgg gattttcctg accgtcaacc tcagcatcgg catgttcacg
     6421 ccgcccttcg gcctcaacat cttcgtgtcg caggcactgt tcaaggcgcc gctgccgacg
     6481 atctaccgcg ggctggtgcc gttcatcgtg ctgcagctga ttgcactggc ggtcgtgacc
     6541 tacgttccgg aggtctcact caccctcgcc gatctggttc ggaagtagcg attccgcata
     6601 ggggcaagcg ttcccttttc tctggtcgcg tgcagctcgg ctgcaacctt atactcgtgt
     6661 ctgggtccaa agcccgagtg gagggattca taatgttcag tcttcgtcac tatcttcctc
     6721 ttgccattgc caccgccctg gcaggcttaa cgggtctgtc cgcgccggcc aaagctcaga
     6781 ctacgatgaa actcgccacc gcgaccgtga acgatgtcca ggtcgaggcg atcaaatatt
     6841 tcggcgccaa ggtggaggcg cgcagtaacg gccggatcaa ggcggaatac taccccggcg
     6901 cccagctcgg ctccaatccg cgcatgatcg aggggctgca acttggcacg atcgaagtct
     6961 atgtcgggcc gcccgcctac atggtcggcc tcgactcgcg tttccaggtg atggacgcgc
     7021 cgggtctgtt cgacgacatg gagcatttct accgggtgac gcaggacaag gaattccgcg
     7081 acacgttcct ggcgctcggc gagcggaaag gcgtcaaggg catcagcctg ttcgtctaca
     7141 gcccgacctc ctttgccacc cgcgacccga ttcgcaaact gccggacttc cagggcaaga
     7201 agctgcgcgt gctcgcctcc aagctggagc gtgagtcggt cggcaaactg ggcgctacgg
     7261 ccgtcccgat cgacttcagc gaagtggtct ccgccctgca acagggcact gtcgacggca
     7321 tgcggactgg cctgtcggta ttcacctcga tgaaggtcta cgaggtggtg aactacgtca
     7381 ccaccacaca cgaggccatg atccccgagg tgatgatggt ctcgaaagcc tggttcgacc
     7441 gccagcccga cgacatcaag caaatcatcg tcgaggaggc ggccggcatg gagcgccatc
     7501 tgttcgactg gaccagccgc cagcaggtcg aggcagagaa aatttggaag gaaaagggcg
     7561 gcgagatcat tcaactgccg gaggccgatc gcaaagaaat gatgcagcag ctggcgcagg
     7621 tcgccgagca ggtgctgagc gagaagccca ccgacaagca gatgttcgag atgctcaagc
     7681 aggcggtcga acgcgcgcgg gggaaataag catgtggaag cttgagccaa ctaaagtcgc
     7741 cgccatccag gcggcgcctg tcttcctcga ttccaccgcc acgaccgaga aggcctgccg
     7801 cctgatcgcg tcggccgcgg agcagggcgc gcgcgtgatc gcgctgccgg aagtgttcat
     7861 ccccggctat ccgtactgga gctggatgat gaacccgctc gaaggcagca aatggttccg
     7921 cgcgctctac aaggcatcga tcctcgtccc cggcccggag acgaacctgc tggggcgcgc
     7981 cgcgcgcgac gccggtgcct acgtcgtggt cggggtgaat gagcgcagcc cgaacagcat
     8041 cggtacgatc tataacacca acctggtgtt cgggccggac gggtcgctgc tcgggcggca
     8101 ccgcaagttg gtgccgacgt ttgcggagaa gctgacctgg gcgagcggcg acggcagcag
     8161 cctgcgcacc tacgagacgc cgtatggcgc catcggcacc ctggcctgcg gggagaacac
     8221 caacaccctg gcgcgcttca cgctgctctc gcaggacgaa cggatccaca tcgcgaacta
     8281 catcgccttt ccgttcaccg agaactacga catgccggag gcaatccgca tccgcgctgg
     8341 cgcgcacgcg ttcgagggca agttgtacac gctggtgtcc tgctcggcga tgtcggacca
     8401 gatcgccgac atgctgtgca cgaccgacga gcagcggcgg atgatgagcg gtcggccgaa
     8461 cgcgttctcg ggaatcttcg gcccggacgg ccggcttgtc accgatccgc tgatcgacga
     8521 ggaaggcatc gtcgtcgccg agatcgatcc ggagcgctgc atcgagccga agcagttcca
     8581 ggatatcacc ggcgcctata accggttcga catcttcgat ctgcgggtga accgccgcgc
     8641 gctcgctccg gcccagttcg cggacgccgc gccggcggag cgatcgcccc agcagctcga
     8701 gcctcagtcc gagcggatgc gggtgagtga gcaggattaa tgtctggcct gctgcgcgca
     8761 cgcccagaga ccccggaggg cttgccggcc aagagccttc cggcgcatta tccgtccatg
     8821 gcggagtcaa aggcggatct gcagggcatc aagtcgatcg aggtgggctt ccgcctgctc
     8881 tcggcactga cggccgcgcg cagcagtctc ccgctctgtg acctcgcgcg ctcgtcgggc
     8941 atgagcccga gcaaggcgca ccgctacctc gcgagcttca tgaagaccgg cttggtcgag
     9001 caggatcccg ccacccgcga ttaccggctc ggcccgttcg ccttcgacat gggccttgcc
     9061 gcgttcggca gctcggacca cctcaggcac gcgatcgacg tccaaatcaa gctgcgtgac
     9121 gagctcgacg agaccgtcgt gctcacggtt tggggctcgc acgggcccac ggtgctgcgg
     9181 gtcgaggaga acagcgatcc cgtcatcatg acgatgaagg tcggtgccac gctgccgctg
     9241 atgcaatcgg ctgcagggcg gatctttgca gcgttcatgc cgcggcggat catcgaaagc
     9301 tacctgcaag tggcaaactt ctccgcttcc gtctacagcc gtcgcgagat cgttcgttac
     9361 gacgacatcg agccggagct ggaggaaatc cgccgcacac gcctgtcggt ctccaacggc
     9421 gaggtgctgc gcggggtcaa cgccgtggcc ggcccgctgc tggcaccgca cggccaattg
     9481 gtaggcgcgc tcggggtgat cggccagcag gaaagcctag acatgtcgtc cgacggccat
     9541 atcgcacgcg tgctgcaccg tgcggtcgtc gcctacgagc gctcggccgc gatcagcgcc
     9601 gccaaccgtc cacggtcgct ggccgcgcct cagagcgatg atgcgcgcca tcgcgcgcgc
     9661 ccaaaccggg agagatgaat gccgctaccg ctgctgccga cgtcgctggt cggctcctac
     9721 gcccagcccg aatggctgat cgatcgcaag aagctggccg gtcgctttcc gccacgcgtc
     9781 cgggcgacgg aattgtggcg tgttgcgccc gaatttcttg ccgaggcgca ggacgatgcc
     9841 acactgctcg ccatccgcga ccaggagcgc gccggccttg atattatcac cgacggcgag
     9901 atgaggcggg agagctattc gaaccgtttt gcgaccgtgc ttgagggcgt tgacatcgac
     9961 aaccctggca ctgcactcga ccgctccggc catcctaacc cggtgccgcg cgtggttggg
    10021 aaaatccgcc gccgcgaacc agtcgaggtg cgtgacgtag agttcctgcg cgccaacaca
    10081 gaccgtacga tcaagataac ggtgccaggc ccgttcacca tgtcccagca ggcgcagaac
    10141 gatttctacg ccagcgagga ggagttggcg ctcgactacg ccggggcggt caatgaggag
    10201 atcaaggacc tgttcgccgc cggagctgac atcgtgcaga tcgatgagcc ctatatgcag
    10261 gcgcgaccgg agaaggcgcg ccagtatggg ctgccggcgc tcgcccgcgc gctcgacggt
    10321 gtgactggca ccaccgccct gcacatctgc ttcggctatg ccgcgatcat ccacgagcgc
    10381 ccgtcaggct actcgttcct gccagagctt gcgcacgcgc cagtgcaaca gatctcgatc
    10441 gagaccgcgc agtcgaatct cgacctgtct gtgctcgaaa agctcccggg caagaccatc
    10501 atcctgggcg tccttgatct ttctgacatg aacgtcgaga cgcccgagac cgtcgccgcg
    10561 cgcattcgcc gtgcgctgcc gcatgtgccg gcggagcgga tcgtggtcgc gccggattgc
    10621 ggattaaaat acctgccgcg cgaggtcgcg ttcggcaaga tgcgggcgat ggtcgaaggc
    10681 gcgaagatcg tgcgccagga gttggcaggg tctcgatgac caggctatta gctcttgccc
    10741 cggtataaag gtggacgtgc cccggccgca gccagatcga aaggcatctt tcgcaaggtg
    10801 actggcggac ggagagtccg ccaatgacgt atgcacgcta gcgtaattgt gcgtaagctc
    10861 ttgtaaccgc gcattttccg agacatattt gtccccggct ttctgcgcgc gccctattac
    10921 ctcggatagg aggggcgtct gccgaacagt tgacgcagcg tgtcgtcatt gtattgcagc
    10981 aggttcgcct ttttcgaata gaagtcgcac gttgatgaaa cctcggtgga gtcggccttc
    11041 aggttcatgg ccctgtagcg ccccgtgtgg tcgcccaccg acattacgac cacgtcaccg
    11101 gtcttagtcg tcataagccg gatgtcacgc tcgtgcagca gcttgcggat ttcatcgcgc
    11161 ggatgatcga actggttatc gtagttggac gagcagatcg cgagggacgg ccttatgtgg
    11221 cttagaaagg gcttgttggt aaagccattg tctgcaccgt gatggctgag aatcatcacg
    11281 tcggtctcac gctgcaggat cgtccgtctg cgcaagtagg ccgacaggtt gtggctctcc
    11341 acatcgccga ggcttagcac attgaaactg cctccccgaa ataatttcac tgtagagttg
    11401 ttgtttgtgc agtcgggatc aatgaagcgc ggatgataaa agacgtcgtc gaaggccagc
    11461 tgcgaggcac tctgaagccc tttgatgtat tctggtgaaa tggtgcgcaa ctcgacgggt
    11521 cggttcgagt tgcgtttcgt ctccttgtaa gccttgatta tccgggcaga ttctctgcca
    11581 ttgtcggtgt gcggctcgta gcctggacat tcgatggtgc gtggtaccgc aagtgccagc
    11641 aggtcgatga gttcggaggc acagcagtgg tcggagtccc agctcgtgat gtggagcgtg
    11701 tcgacagcct tcactccgca ggcggccatc tcttggatga tggtggcctt gttagtgtcc
    11761 gttagccttg cctcgatgag cgtaaaatgc ccgcccgcga agtacgagaa cgacgacccg
    11821 ggcgagccca gctgatacgc gcgaaagcgc gtgcgcacgg ctttcaaagt gccccccata
    11881 aaccttgagc tttgcccgac tatgtggtgt cggttgccgc aaatgacaac cgatatccaa
    11941 cgggggatgt ggatgaaaat cggcctcgcg ctgtcgggtg gcggcatgcg cgccgcggct
    12001 ttccactgcg gcgttctcag gcgtcttgcc gaacaggatc tccttgagaa cgtggcgcag
    12061 ctgtcgaccg tttcaggcgg gagcctaatg gtagcggcgc tcttgggctt cgcggaaaat
    12121 aagtggccca catcggggga attccgcgac cgcatctaca gttcggcgcg cacgctgttc
    12181 acgaccaccg acctgttgag tctgcgcgca atcgggctaa gtggcgcagt gcgcttcaac
    12241 gccagacttc tcacgcaccg ggcaaccgtc ctcgccgaca tgctggaacg gcgctggcgt
    12301 gtgagcggta tgctggccga cctgcccgag cagccaatat ggtggatcaa cgccacctct
    12361 gtcgaaacag gcaa
//
LOCUS       DASVDU010000036        11790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1105447, whole genome shotgun sequence.
ACCESSION   DASVDU010000036 DASVDU010000000
VERSION     DASVDU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11790
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105447"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VF226_02540"
     CDS             complement(<1..1020)
                     /locus_tag="VF226_02540"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7072892.1"
                     /translation="MSAGRGGGAASKLLIALAVAGAMGTAGVGGYVLSDFVRRENPPA
                     PAASAPVEEQWIASAPGQVEAKSGEIRIGAAIPGRIAAVYVSANDRVERNEVLARLED
                     DEARARLSAAEAEAGARERERNAGPATEGREDVRKAADAVYRAERALTGARYELDAAL
                     LNKREGSGDDRHIADARQRLTEAQERFQRERIQLANAQARSDLPAPNRLEAALIAARA
                     EVSLAQVVLDRTRIRGPIDGTVLGVLGKVGETVSSSPDQPILTMSDPNVVVVKAEVDE
                     RDIAKVQLGQRAFVRSSAFPGQEIEGKVTAMAPVLAGPHMTSRGPRRPDDAEVREVTI
                     EFEDAG"
     gene            complement(1065..1886)
                     /locus_tag="VF226_02545"
     CDS             complement(1065..1886)
                     /locus_tag="VF226_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072893.1"
                     /translation="MLLTPEDTVEPHQDATHGGSIRVLQRDDWRATTTRPSKPILRAR
                     GIVKVLGTGANELQVLKGIDLDLRPGELTLLMGPSGSGKTTLLSILGCILTPTSGDLE
                     VAGQSTKGLGPEGLADLRRKHVGFVFQSYNLFPTLTATQNLLLALDLRGIKGQMASEY
                     AARALAEVGLTHRANAYPGQMSGGEKQRVAIARALSGSSSVILADEPTAALDGENGKA
                     VMALLSEVAQDPSRAVLAVTHDHRTLAYADRIIRIEDGRIVDDKASEGIRAGAGR"
     gene            2723..3046
                     /locus_tag="VF226_02550"
     CDS             2723..3046
                     /locus_tag="VF226_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072894.1"
                     /translation="MLGWSGTGPAEQFEDLVRQFWTAGGSNGPTRYMEAMARANVEMV
                     GLLGRRSRAYLELPTQLSRCRTPQQMLEEQAKFFQDMLHDYQVTNDRVVNCWIEATGA
                     PAEQR"
     gene            4049..6889
                     /locus_tag="VF226_02555"
     CDS             4049..6889
                     /locus_tag="VF226_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7072895.1"
                     /translation="MRRILQAENSAPANPGSFDRRAMLNFMTASLALGGLAGCAVNRQ
                     PFGAPLLSQPFRSPQHTPGVPLFFATSLELDGLGRGVLVKSHDGRPVKIEGNPQHPAS
                     LGATDVFSQAEVLSLYDPDRSKHPIAGGERRSWTDARQLIAQLRQQFLAKKGRGLRIL
                     LQPCASPTRDRLIRLVRQAFPEARWHAYSPLPDDQGRAATRAVFARPLDQVFALDRAD
                     VILTLGGDLLGSGPGHVRYAADYADRRRAADRSPPVLYTVETTPSLTGARADRRLAAA
                     PREIEHFARQILIHLEMGAPATPDQLSVARRAAEDLARAGPHALVAAGREQPVTVQAI
                     AHALNSRLGAIGTTVRYIEPLFAGSGEMETLTELASAIAAGDVEALLILGGNPVYDAP
                     TDIEFAKMLGSVPLSIHVGTYRDETGLACRWHLPARHPLESWGDLRAFDGTVGLRQPA
                     TVPLIEATTPEELLGELAGEAADARELVQATWRAEWGADFDARWITSLEQGLIEGSGE
                     VTVPVQPGDWSPPPATRSPPAEGQTVVFAPDPSVWDGRFANNAWLQELPKPLSKQVWG
                     NAAFMAPADASALGLRGGDIAVFRHNGRSIEAPVWPLPGHAQNAVTVTLGYGRTAAGK
                     IGNSRGFDAYRLRTSQEPWIIENVDVLSTGRRERLVTTQHHHSMEGRDIVRVVAPGAA
                     SASSSVSPPPSFYPDFVYDGNAWGMTIDLDVCLGCNACVIACQAENNVPVVGPEEVAR
                     GREMHWLRIDRYYSGHPNSPQTYFQPVPCMHCEKAPCELVCPVNATVHSSEGLNEMTY
                     SRCVGTRTCSNNCPYKVRRFNWFDYSRLPFAATDDVFNPEVTVRWRGIMEKCTYCVQR
                     ISATRIAARIENRPIRDGELMTACQQACPTRAIVFGDINDPASLVRKQKQDPRNYALL
                     GELNTQPRTTYLARISDGPSAQGAADAHDDAG"
     gene            6873..8186
                     /gene="nrfD"
                     /locus_tag="VF226_02560"
     CDS             6873..8186
                     /gene="nrfD"
                     /locus_tag="VF226_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7072896.1"
                     /translation="MTMPAEAPARDIARIPLSPDFGWRWAASLIASVAMVFVLAYAVG
                     LAVFVGVGVWGINVPFVWGFDLTNYVWWIGLANGCSLFAAILVLRRHSLRTAINRFAE
                     GLALFAVVCAGVFPIIHLGRPWLFYWMFPYPAEFQVWPQFRSPLTWDFWAIITHLMVT
                     TMLWYVGLIPDLATLRDRARSVAAKRVFAVFALGWRSSIRHWAFHQRAYRLVAIAVIP
                     LVLVIQSTIAFEFAVTLVPDWHETRLPLHFIVSGLESGLALILFAAAALRTSLRLERY
                     IDDEDMDLIGKLLLANALVSAYVYAGEIGVALFADDFAQTAALNRMLGDYASLYWGTI
                     ALSVIVPQVLWLRNVRLNDGLSLLVGLAVTAGIWLDRLSLVVAGIQRDYLPSMWRTYS
                     PTAPEWYLFLGSIGLIAALFLLFVRYLPVISMSETRHDEHLESRS"
     gene            8183..8722
                     /locus_tag="VF226_02565"
     CDS             8183..8722
                     /locus_tag="VF226_02565"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX7072897.1"
                     /translation="MSRNDYGIIGEFETADQLIAATRAARDTGYRRIEAFAPFPLLNV
                     SRDLGCRTVIVPVIAATAAVVGGAITYFSEYWMNGIDYPLNVGGRPLNSWPAFIPATI
                     IVSAIWLATAAFVTMLVKCRLPRLNHPVFVVPDFERASEDRFFLSVLAEDPAYEPRDV
                     TALLEKHGAVAITRVELTG"
     gene            8719..9237
                     /locus_tag="VF226_02570"
     CDS             8719..9237
                     /locus_tag="VF226_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7072898.1"
                     /translation="MIRAAAALCPALLLLAGCDAAPSDGTPVTARRARTEFRIAVPPG
                     AVPSGTAEAMRQLAGPGPAITAELLSAGRNRYDAFCAPCHGLTGYGDGVVVQRGFPAP
                     PSFHAQPQQALTRSRIVEVITQGSGLMFPFAERVPPQDRWAIASYVKALQLSQSAPLD
                     RLPEDLRRQVGQ"
     gene            9234..10307
                     /locus_tag="VF226_02575"
     CDS             9234..10307
                     /locus_tag="VF226_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072899.1"
                     /translation="MNGSDAEAGTHYVTALQALTYGTVVLLAIVFAIGWRTEALLEAW
                     LVAFLVLAGLASASLGLLMIGHLLGETWLHPVRCPLEAAASTTPLVLFLALPLAVGAD
                     LLYPWAAGPIRGIAPERQLYLSESFVLVRGAVILVLWIAIAIPILRRGRHQKASAIGL
                     ALLAPTAAIFSIDWVGSREPVWWSSLFAFAYTVTQLAGAMALALVVAMLQQSYPDPAR
                     YHSLQRAAITLALLTFWVWFAQFLVVWMANLPAEVEWYLARSEGAWRWPMMAVALPSL
                     LASIVLLLPSHPSRWRLVAAGLFLFVQYLAHMLWLVRPASAGMPALTWADPPVWLGLA
                     LLWAIWYMAARRVAQATIGQPPA"
     gene            complement(10285..10857)
                     /locus_tag="VF226_02580"
     CDS             complement(10285..10857)
                     /locus_tag="VF226_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7072900.1"
                     /translation="MQLDLRNLGSALSIGLAISACDASREPPPHLRIEGGDATVGRQL
                     IVNNGCGTCHVIPGVPGAIGMVGPPLNDFAQRTVLAGTYPNVPRWLVPWLMDPPALQP
                     ATAMPNLGLSAEEAGHIATYLYTLGAAGVAPYEYRPATSQYPWLEEADAYREAETRRL
                     FETERIGTGKARIPIERAMELLTTTPEAGR"
     gene            complement(10960..>11790)
                     /gene="mgtE"
                     /locus_tag="VF226_02585"
     CDS             complement(10960..>11790)
                     /gene="mgtE"
                     /locus_tag="VF226_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7072901.1"
                     /translation="VVDEQRRLTGVVSLRDLILARPSAKIADIMDTHVICARAEDSQT
                     EVARLIAKYDLIALPIINGGDALVGIVTQDDAMDVAEEEATKDFHKVGTVGAIAISLK
                     EASIGLLYRKRITWLVVLVFFNIFSGAGLALFQDTIVSHVALVFFLPLLIAGGGNAGA
                     QASTLMVRALATGDVRLADWGYMLARELVVAAALGLTMGLAVAMIGIARGGPQIALIV
                     AATMVLVVITGSIIGMSLPFVLSRFKLDPASASAPLITSIADVAGVLIYLSIATFVLG
                     "
BASE COUNT         2036 a         3740 c         3662 g         2352 t
ORIGIN      
        1 cccggcgtcc tcgaactcga tcgtaacctc gcgcacctcg gcgtcatcgg gccggcgggg
       61 tccccgtgat gtcatatgcg gaccagccag gaccggcgcc atcgcggtta ccttgccctc
      121 gatctcctgg cctggaaaag ccgagctgcg tacgaacgcc cgctggccaa gctgcacttt
      181 ggcgatatcc cgctcgtcga cctcggcctt cacgaccacg acgttcggat cgctcatggt
      241 caggatgggc tgatcggggg aagacgaaac cgtttctcca accttgccca acaccccgag
      301 cacagtaccg tcaatcggcc cgcggatgcg tgtacgatcc aacacaacct gtgccaacga
      361 gacctctgcg cgggctgcga ttagcgccgc ctctagtctg ttcggcgcag gcaaatccga
      421 gcgtgcctgg gcattggcga gctggatgcg ctcccgctga aaccgctcct gtgcctcggt
      481 gaggcgctgc cgggcatcag caatgtgacg atcgtcaccg ctcccctcgc gcttgttgag
      541 cagggcggca tcgagctcgt agcgggctcc tgtgagggcg cgttctgcgc ggtagaccgc
      601 atccgcagcc tttcgcacat cctcccggcc ctccgttgcg ggacctgcat tgcgctcgcg
      661 ttcgcgggca cctgcctcgg cctccgcagc cgacaaccgc gcccgagctt cgtcgtcctc
      721 gagccgggcc agcacctcat tcctttccac ccgatcattg gcgctgacat acaccgcggc
      781 aattctgccc gggatggcgg cgccgatccg tatctcgcct gacttcgcct ctacctgtcc
      841 gggagcggaa gcgatccatt gttcctcgac tggcgcactc gcagccggtg ccggtgggtt
      901 ctcgcgccgc acgaagtcgc tgagcacgta gccaccgacg ccggccgtac ccatggcgcc
      961 cgcgacggca agcgcgatga gaagcttaga tgcggctccg ccgcctcggc cggcgctcat
     1021 ggtcttcgcc tccttcgctt atctggccgt cttttccggc tgacttatct tcccgccccg
     1081 gccctaatgc cctctgaggc cttatcatcg acgatcctgc cgtcctcaat tctgataatg
     1141 cgatcggcgt aggcgagcgt gcgatgatca tgcgtgactg ccaaaacggc tcgtgacgga
     1201 tcctgagcca cttctgacag gagcgccatc actgctttgc cgttctcgcc gtctagggcc
     1261 gccgtaggct catcggccag gatcaccgaa gaggagcctg aaagcgcgcg agcgattgcc
     1321 acccgctgtt tctcgccgcc gctcatctgc cctggatagg cgttcgcccg gtgagtgagg
     1381 ccaacctcgg ccagcgcgcg tgccgcatac tcgctcgcca tctgtccctt gatcccacgc
     1441 agatcgaggg cgagtagcag attttgagta gccgtcaggg tcggaaaaag attgtaggac
     1501 tggaacacga agcccacgtg ctttcgacgc aggtcggcca atccctcggg gccgaggcct
     1561 tttgtcgatt gacccgccac ctccagatcg ccgctggtgg gcgtcaggat gcatccgaga
     1621 attgacagca gcgtggtctt gccgcttccg gacggaccca tcaaaagggt cagctctccc
     1681 ggccgcaggt cgaggtcgat tcccttgaga acctgaagct catttgcgcc ggttcccaga
     1741 accttgacga tgcctctggc gcgcagaatg ggtttggaag ggcgcgtggt ggtggcgcgc
     1801 caatcatccc tctggagcac ccggatgctg ccgccgtgcg ttgcgtcttg gtggggctcg
     1861 actgtgtctt ccggcgtgag cagcatgcct gttttccttg aaaatcgcga tggtcttgcc
     1921 gccttcgccc ggtggcccgc ttctttgcag aagatcacgg gctagagcag cagcctcaat
     1981 atgatgttgg ctgggatgga ccggccacgc tgttccagca gtgacaagtc ctcgacgttg
     2041 cccgggcggg ccattcagca gaactgccaa tcagacacca caagcgcgca atcgtgacga
     2101 aaagatgagg attgcgggcg tgtcgcacgc agcaaacgtc gcaggcgacg ctcgacgagc
     2161 agagcctgga ggcatcgagg caggccttca gttttattga acggcaattc acgtaaggcc
     2221 cttggcgatc tctcgtgtgc cgggtggaaa ccaaacaccg ttgcgctcgt ttatcatcgg
     2281 atcggaagcc aaaacgatct ggctttccaa ctcagtgggt acaccttgca acgaatgctg
     2341 cagcccgcac gcaccggacg cgccggctcc gggtggatcc ggccggggat gacaagaggg
     2401 atctcgttct tcagcgctcg ggcttccgag caatcctgaa cccatgaccg ggaggttgat
     2461 cgtgccagcg aaatcaaagc gcactcagtc gagtagaggc cggcaacagt caactcaaca
     2521 gcgcaaggaa ccgcaaaagc gccaggcaag gcgggcgcag gagcggtcga gagccgctcc
     2581 tgaaacgaac ggtcacgaag aggcggctct ttccggaggt ttcgagcagc cgtttagggc
     2641 ggcctcgcag tttgcgcgtg ccgcaagcga gcagatcaaa caggcagcgg gcgagggcgg
     2701 ttgggcccgt gcgaggccgc aggtgctcgg gtggtcgggg actggcccgg ccgagcaatt
     2761 cgaggacctg gtgaggcagt tctggacggc tggcggctcg aacggaccta ctcgctacat
     2821 ggaagccatg gcgagggcga acgtggagat ggtggggctc cttggtcgcc ggagcagggc
     2881 ctacctcgag cttccgacac agctgtcacg ctgccggacg ccgcagcaga tgctggaaga
     2941 gcaggcgaaa ttctttcagg acatgctgca tgattaccag gtcaccaatg accgtgtggt
     3001 gaattgctgg atcgaagcga ccggcgcccc cgccgagcag cggtaaatgc ttcgatctta
     3061 cctctttccg cggcaatggc ttgtcggctg gccattgccg gccggcaagt agctcgctgg
     3121 tcgcgaaagc aattccatcc ttttctcttc ccggaacaag acaagcgccc gttcgtttcc
     3181 gccctgagcg gactgcattg tgctggcgcg tccaaggaga aaaggatctg ctgcgcgcca
     3241 ccgagccata tcggtgacat gcgatggcgc agctcttccg gccggaagcg aacacagcat
     3301 tccgattagg acttgtggca gccgccttgg gcttaatcct cattctcgca ggcggttacg
     3361 tgtacgtccg atcagaatgg ttctggaatg tcggtgagag tgctcctcaa cccattccgt
     3421 ttcggcatga tcttcatgtt tcgggcatcg gcatcgcctg cgtcttttgc catagcaccg
     3481 ccgagcgcgt ggcgcctgcc ggaatgccaa ccgcccagac ctgcctcacg tgtcattcgc
     3541 aaatcctgca gggcgcaagc gtgctcgagc ctctgcgaac gagcgctgcc ctgaaccggc
     3601 caatccgctg gtcctcggtg agccgattgc caaacttcgt ctatttccat cacggcgttc
     3661 atgcgagcgc cggcatcacg tgcgaaacat gccatgggcg catcgatcag atgcgtcaga
     3721 cgcggaaagc gcactccctt tcgatgggct ggtgcctcgg ctgccaccgt gtttctgagg
     3781 ctgcagcgga ggccggcaaa gataagcggc aggtcaaagc ggacgcagta tccaactcgc
     3841 ccagcaacca gcgctcgcta aaagtcagcg agcatttcgc gacacccctc accaattgct
     3901 cggtttgcca ccgttagtca tggccaaaaa tcgcgcgctg ccagttgacg cttacatccg
     3961 ggacaggccg gaactggcgg gtccgcactc aaacgccaat gacctccgat tatggcgtag
     4021 cctcgaggag gcaggagaat gcacgaaggt gcggagaatt ttgcaggccg agaattcggc
     4081 gcctgcaaat cccggctcct tcgaccggcg tgcaatgctc aatttcatga cagcctcgct
     4141 ggcgctgggc ggcctcgccg gctgcgccgt caaccggcag ccttttggtg cgcctcttct
     4201 ctcgcaaccc ttccgctcac cgcagcacac gccgggtgtc ccattgtttt ttgcgacgtc
     4261 gctggaactc gacgggctcg gccgtggcgt gctggtcaaa agccatgacg gcaggccggt
     4321 aaaaatcgaa ggcaatcccc agcaccccgc cagcttagga gcaacggacg tcttttccca
     4381 ggcggaggtg ctttcgcttt atgaccccga tcgatcgaaa catccgatag ccggagggga
     4441 gaggcggagc tggacagatg cccggcagct gatcgcacaa ctccggcagc aattcctcgc
     4501 gaaaaagggc cggggacttc gcatcctgct gcaaccatgc gcgtcaccga cgcgggatcg
     4561 gctcatcagg ctggtgaggc aagccttccc cgaggcgagg tggcacgcct attcgccgct
     4621 gccggatgac caaggtcgtg cggcaacgcg cgcggttttc gcccggccgc ttgatcaagt
     4681 cttcgctctc gatcgggcgg acgtgatcct gacgctcgga ggagatctcc tcggttcagg
     4741 cccaggccac gtccgttatg ctgccgatta tgctgatcgc cggcgtgcag ccgaccgctc
     4801 cccgccagtt ctttacactg tggaaacgac gccaagcctg accggggcgc gcgcagaccg
     4861 gcggctcgcg gcagctccgc gggaaatcga gcactttgcc cggcagattc ttatccacct
     4921 ggaaatgggc gctccagcca cgccggacca actttctgtc gcccgacgtg cagctgaaga
     4981 cttggcgcgg gcaggaccgc atgcgctggt cgccgcgggg cgcgagcagc ccgtaacggt
     5041 ccaggccatt gcgcacgcgt tgaacagcag gctgggggcc atcggcacca ccgtgcgcta
     5101 catcgagccg ctctttgccg gcagcggcga gatggagacg ctgacggagc ttgcatcagc
     5161 cattgctgca ggagatgtcg aggccttgct gatccttggc ggcaaccctg tgtacgacgc
     5221 gcccacggat atcgagtttg cgaagatgct cggcagcgtg cccctctcca tccacgtcgg
     5281 aacctatcgc gacgaaacgg gacttgcatg ccggtggcat ctgccggcgc gtcatcctct
     5341 cgagagctgg ggcgacttgc gcgcgttcga tgggacggtt gggttgcgcc aacctgcaac
     5401 ggtgccgctc atcgaagcga cgactccgga ggagctgctt ggggagcttg cgggcgaggc
     5461 agcggacgcg cgcgagctgg ttcaggcgac ttggcgcgcg gagtggggtg cagatttcga
     5521 tgcgcgctgg atcacgtcgc tggagcaggg cctgatcgag ggcagcggag aagtcactgt
     5581 cccggtgcag cctggcgatt ggtctccgcc tcccgccacg aggtcaccgc ctgccgaggg
     5641 gcagacagtc gtgttcgccc ccgatccgtc cgtatgggac ggccgcttcg cgaacaacgc
     5701 gtggcttcag gagctcccga aacctctgtc gaagcaggtt tggggaaatg cggcgttcat
     5761 ggctcccgcc gacgcatcag cgttgggttt gcgtggaggc gatatcgcag ttttccgtca
     5821 caacgggcgc agcatcgaag cgccggtctg gccgttgccg ggccatgccc agaatgcagt
     5881 tacggtgacg cttggctacg gtcggaccgc tgcgggaaaa atcggaaact cgcgggggtt
     5941 cgatgcttat cgcttgcgca cgtcgcagga gccctggatc atcgagaacg tcgacgtact
     6001 ttccacggga cgtcgcgagc gactggtcac aacacagcat catcactcca tggaggggcg
     6061 ggacatcgtc cgcgtcgtcg cgcccggagc ggcaagtgcc tcctcgtcag tatcaccgcc
     6121 gccatcgttt tatccggatt ttgtttacga cggcaacgcc tggggcatga cgatcgatct
     6181 cgacgtctgc ctgggctgca acgcctgcgt catcgcctgt caggccgaaa acaatgtgcc
     6241 cgttgtcggc ccggaggaag ttgcgcgcgg tcgcgaaatg cactggttgc gcatcgatcg
     6301 ctattactca ggtcatccaa atagcccgca aacctatttt cagccggtgc cctgcatgca
     6361 ctgcgaaaaa gcgccgtgcg agctcgtctg cccggtgaat gcaacggtgc attcgagcga
     6421 gggcctcaac gagatgacct acagccgatg cgtcggcaca cggacctgct ccaacaactg
     6481 tccctacaag gtgcgccgtt tcaactggtt cgattattcc cggcttccat tcgcggcgac
     6541 ggacgatgtt ttcaatccgg aggtcacggt tcgatggcgc ggcatcatgg agaagtgcac
     6601 atattgcgtc cagcggatca gtgcaacccg catcgctgct cgcatcgaga acaggccgat
     6661 ccgcgacggc gagctgatga cggcgtgcca gcaggcttgc ccgacccgcg caatcgtttt
     6721 cggtgacatc aacgatcccg ccagccttgt acgaaaacaa aagcaggacc cgcgcaatta
     6781 cgctttgttg ggagaactga acacgcagcc gcgcacgacc tatctcgccc gtatctcgga
     6841 tgggccgtcc gcccaaggtg cagcagacgc tcatgacgat gccggctgaa gcgcccgccc
     6901 gggacatcgc ccgtattccg ttgtcgccgg acttcggctg gcgctgggcc gccagcctca
     6961 tcgcctcggt ggctatggtt ttcgtgctgg cgtatgcggt cggacttgct gtctttgtcg
     7021 gcgtgggcgt ttggggcatc aacgtgccgt tcgtctgggg tttcgatctc accaactatg
     7081 tctggtggat cggattggcg aatggctgca gcttgttcgc agcaattttg gtcctgcgcc
     7141 ggcatagtct gcgtacagcc atcaatcgat ttgccgaggg gctcgccctt ttcgctgtgg
     7201 tttgcgccgg tgtctttccg atcattcacc tcggccggcc gtggctcttc tactggatgt
     7261 ttccatatcc agcagagttt caggtctggc cgcagttccg cagtccgctg acctgggatt
     7321 tctgggcgat catcacgcat ttgatggtca cgaccatgct ctggtacgtc ggcctgatcc
     7381 cggatctcgc gactctacgc gaccgtgccc gttcggtggc cgcgaagcgc gtttttgctg
     7441 tcttcgcgct gggctggcgc agctcgatcc ggcactgggc ctttcatcag cgcgcctatc
     7501 gcctcgttgc aatcgcggta attccgctgg tgctcgtcat ccagagcacg atcgcattcg
     7561 agttcgcagt cactctggtt cccgactggc atgaaacccg cctgccgctg cacttcatcg
     7621 tcagtggact cgagagcggg ctggccctga tcctcttcgc ggctgcagct ctacgcacct
     7681 cgttgcgcct cgagcgctac atcgacgacg aggacatgga cctcatcgga aagctgctgc
     7741 tcgcgaatgc gctcgtctct gcctacgttt acgcaggcga gatcggggtc gcgctattcg
     7801 ccgacgattt cgcccagact gccgccctga accggatgct gggtgattac gccagccttt
     7861 actggggcac cattgcgctg tcagtgatcg ttccgcaagt cctgtggctg aggaatgtgc
     7921 ggctgaatga tggcctctca ttgctcgtcg gacttgccgt gacggccggg atctggctcg
     7981 accggctctc tctcgtcgtc gccggcatac agcgcgatta cctgccgtcg atgtggcgaa
     8041 cttattcccc gaccgcgccc gagtggtatc tttttctggg aagcatcggc ctcatcgctg
     8101 cgcttttttt gcttttcgtg cgctacctgc cagtgatttc catgtctgaa acgcgtcacg
     8161 atgagcattt ggagagccgc tcgtgagccg caacgactat ggcatcattg gcgagttcga
     8221 aacggccgat cagctcatcg cagcgacacg cgccgcacgc gataccggct accgccggat
     8281 cgaagctttc gcaccctttc cgctcttaaa cgtttcgcgt gacctcggtt gcagaacggt
     8341 catcgtgccg gtcatcgcag cgacggcggc ggtggttggt ggtgcaatca cgtattttag
     8401 cgaatattgg atgaacggga tcgactatcc gctcaacgtc ggcggcagac cgctcaatag
     8461 ctggccagcc ttcattccgg caacgatcat cgtttccgca atatggctcg caacggcggc
     8521 gttcgtgacg atgctggtca aatgccggct tccaaggctc aatcatccgg tttttgtcgt
     8581 gcccgacttt gaacgggcat cggaagatcg cttcttcctt tcggtgctgg ccgaggatcc
     8641 ggcctacgag ccacgcgatg tgacggcatt gctcgagaag catggggccg tagcgatcac
     8701 gcgagtggag ctcaccggat gatcagggcc gccgctgcgc tctgtcctgc gcttttgctg
     8761 ctggccgggt gtgatgcggc gccctcggac ggcactccgg tcacggcgag acgcgcccgt
     8821 acggaatttc gcattgccgt gccgccgggc gccgtcccga gtggcacggc cgaagccatg
     8881 cgccagctcg ccgggccggg gccagctatc acagctgagc tcctttctgc agggaggaac
     8941 cgctacgacg cgttctgcgc gccctgtcac gggttgacgg gttatggtga cggcgttgtg
     9001 gtccagcgcg gttttccagc accgccatcg ttccatgccc aaccgcagca agcgttgacg
     9061 cgttctcgca tcgtcgaggt gatcacgcaa ggaagcggct tgatgtttcc gttcgccgag
     9121 cgggtgccgc cgcaagatcg ttgggcgatc gcgagctacg tgaaggcgct acagctcagc
     9181 caatccgctc cgctcgacag attgccggaa gacctccgcc ggcaggtggg acaatgaacg
     9241 gttcagacgc ggaggctggc acgcactacg tcacggctct gcaggcgcta acgtatggca
     9301 cagtcgtttt gctcgccatt gttttcgcaa taggttggcg gactgaggca ttgctcgagg
     9361 cttggctcgt cgcgttcctg gtgcttgcgg gcctggcatc ggccagcctc ggcctcctta
     9421 tgatcgggca tcttctcggg gagacgtggc ttcatcccgt acgatgtccg ctcgaggccg
     9481 cggcatcgac gacaccgctc gtccttttcc tggcgcttcc tctggcggtc ggcgccgacc
     9541 tgctttaccc atgggcggcc ggtccgatac gcgggatcgc gcccgagcgg caactctatc
     9601 tgagcgaatc gttcgtcctg gtgcgcggag ccgttattct cgttttgtgg atcgccatcg
     9661 ccattcccat tctgcgccgg ggccggcacc agaaagcaag tgccattgga ctggcgctgt
     9721 tggcgcctac cgccgcaatc ttttcgatcg attgggtcgg ctcacgcgag ccggtctggt
     9781 ggtcgagtct gttcgcattc gcatatacgg tgacgcagct cgccggggct atggcgctcg
     9841 cgctcgtcgt agccatgctg cagcagagct atcccgatcc ggcccgctat cacagtcttc
     9901 agcgggcagc gattactctg gcgctgctga cgttctgggt ctggtttgcc cagttcctcg
     9961 tcgtctggat ggccaacctg cctgcagagg tcgaatggta cttggcgcgc tcggaggggg
    10021 catggcgctg gccgatgatg gccgtagcgc ttccctctct cctcgcttcg attgtgctgc
    10081 tccttccttc tcatccaagc cgctggcggt tggtggcggc cggcctgttc ctgttcgtgc
    10141 agtatctcgc ccatatgctc tggctcgtgc gaccggcatc cgcgggcatg ccggcgttga
    10201 catgggccga cccgccggtc tggctcggcc tcgccctctt gtgggccatc tggtacatgg
    10261 ccgctcgccg tgtagcgcag gcgactatcg gccagcctcc ggcgtagtgg tgagcaattc
    10321 catcgcgcgc tcgatcggaa tgcgagcctt ccctgttccg atgcgctcgg tttcaaaaag
    10381 tcttcgggtt tcggcctcgc gatatgcgtc agcctcttca agccaggggt actgggatgt
    10441 ggccggccga tactcgtagg gcgcaacacc cgccgcgcca agggtgtaaa gataagttgc
    10501 gatatggccg gcctcttccg ccgacagtcc aagattcggc atggccgtcg cgggctgtag
    10561 cgccggcggg tccatcagcc aaggaacgag ccagcggggc acgtttgggt aagtgcccgc
    10621 caggaccgtt cgttgtgcaa aatcattgag cggcggaccg accatgccga tcgctccggg
    10681 aacgcctgga atcacatgac aggtcccgca accattgttg acgatgagct ggcgaccgac
    10741 ggtcgcgtcc ccgccctcga tacggaggtg cggaggtggt tcccgcgaag cgtcgcatgc
    10801 cgatatcgcc aggccgattg acagagcgga gcccagatta cggaggtcaa gctgcatcag
    10861 ccctgctcga tttgatcggc tgccgcacgg ctttcaaacg catccgggga ctatcggttg
    10921 cctcgcgcca gcctggtcac cgccgcgctg cgctccatct cagcccagta cgaacgtggc
    10981 gatgctgagg tagatgagca cccccgcgac gtcggcaatt gacgtgatga gcggtgcgct
    11041 cgcagacgcc ggatccagct tgaatcggct gagtacgaac ggcaacgaca tgccgatgat
    11101 actgccggta atcaccacga gcaccatcgt cgccgcgacg atcagggcga tttgcggacc
    11161 gcctcgagcg atgccgatca tggcgacggc aagccccatg gtgaggccga gagcggctgc
    11221 aacgacgagc tcccgggcca gcatgtagcc ccagtcggcg aggcgcacgt cgcccgtcgc
    11281 caatgcacgg accatcagcg tcgaggcctg tgcgccggcg ttgccgccac cagcaatgag
    11341 cagaggcagg aaaaagacca gcgccacgtg cgacacgatg gtgtcctgaa acagcgcaag
    11401 gcccgcgccc gagaagatgt tgaagaacac gagcacgacc agccacgtga tccgcttccg
    11461 gtagagcagg ccgatgctcg cctctttcag actgatggcg atcgccccga cggtgccgac
    11521 tttgtggaag tccttcgtgg cctcctcttc ggcgacatcc atcgcgtcgt cctgagtcac
    11581 gatgccaacg agcgcgtcac cgccgttgat gatcggcagc gcgatgaggt cgtacttggc
    11641 gatgagccgc gccacctctg tctggctgtc ctcggcgcgg gcgcagatga cgtgcgtgtc
    11701 catgatgtct gcgattttcg cgctgggcct ggcgaggatc aggtcgcgca aggagaccac
    11761 gcccgtcagc cgccgctgct catcgacgac
//
LOCUS       DASVDU010000037         7567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_558116, whole genome shotgun sequence.
ACCESSION   DASVDU010000037 DASVDU010000000
VERSION     DASVDU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558116"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VF226_02590"
     CDS             <1..651
                     /locus_tag="VF226_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7072902.1"
                     /translation="VLRRYDILMIADEVICGFARTGNWFGSQTFGIQPDTITMAKAIT
                     SAYMPLGAVTVSEPVYEAMVDESKKLGVFAHGFTYSGHPVSCAVAVKTLEIYERINIV
                     GHVQSVAPVFETRLKALRDHPLVGETRGVGLIGGVELVKDKATKESFDVRQGLGPKVV
                     KLAEEEGLICRAVSGDTIALCPPLVIDGAEVNQMFDALGRALDRAEEWVNKEGLRG"
     gene            1056..1796
                     /locus_tag="VF226_02595"
     CDS             1056..1796
                     /locus_tag="VF226_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072903.1"
                     /translation="MWRHTFGSERRAPARRRPVQFQADIVVLQGVTNLGVVRQMLPAS
                     LYRVVASRQILENVHATRDAPQVQTTAIAIRRDARLRTVAHEHMLELAEPPPGAETPL
                     SAGIAMRVLGNRRPFWVLSVDLAQGCSGSTVQDQPQCAAAKRQLDLVEAWLSEKVAAG
                     EAAIIAGRFPIEHGEGSLPGKLGQLTGSNGKLAGTCPDGGKSIARTYVLIAPSSRPDA
                     EVTFQGHVEPVDKRKPEWGCALLADVSF"
     gene            complement(1807..2565)
                     /locus_tag="VF226_02600"
     CDS             complement(1807..2565)
                     /locus_tag="VF226_02600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072904.1"
                     /translation="MPAVSNAERKTLILTGASRGIGHATVKRFASAGWRVITCSRHPF
                     PENCPWEMGPEDHIQVDLSNAEDTLRAIVEMRRRLEAQGSRLNALVNNAGISPKGPEG
                     ARLGTIDTALEDWQTVFQVNFFAPIMLARGLIAELEAAKGAVVNVTSIAGGRVHPFAG
                     AAYATSKAALAALTREMAHDFGPRGIRVNAISPGEIDTSILSPGTDKIVREQIPLRRL
                     GEPEEVAKAIYFLCTDQSSYVNGAELHINGGQHV"
     gene            complement(2688..3344)
                     /gene="pdxH"
                     /locus_tag="VF226_02605"
     CDS             complement(2688..3344)
                     /gene="pdxH"
                     /locus_tag="VF226_02605"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEX7072905.1"
                     /translation="MADTPKPEYYTGPQAGDFTAADEPFALFDEWFKDAAAHEPSDPN
                     AMALATVDADGMPNVRMVLLKGVDGPERSDRGFVFFTNYESAKGRELLASRKAALCFH
                     WKSLRRQVRVRGLVSPVAPEEADAYFATRARGSRIGAWASAQSRPLESRFALEKAVAF
                     YTAKYAVGEIPRPPYWSGFRVVPVEMEFWHDRPFRLHDRVQFRRAAVGEPWSRTRLYP
                     "
     gene            3481..3921
                     /locus_tag="VF226_02610"
     CDS             3481..3921
                     /locus_tag="VF226_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072906.1"
                     /translation="MRKRDGAFIRVAACALALALPIAASADDIRSGASNRGSACSCPE
                     LRRLPPPNVSNPAATFTLNELDEIATLNALHIGLTEVPDGANFVWRRSHGRLSGTVRP
                     LFSFKNAKGEVCRHVVVTLRAGDRTRTVEGAACREPNGRWVLEG"
     gene            4020..4910
                     /locus_tag="VF226_02615"
     CDS             4020..4910
                     /locus_tag="VF226_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7072907.1"
                     /translation="MSEWLWVIFTVLAAGGQTLRNAMQKELTTTLGTVGATHVRFLFG
                     LPFGLLFLLMVLLATGEPIPTLSGSMLTWTIVAALAQICATALLLAAMLERSFVVTIT
                     LSKTEPVQVAVFGLAFLGDHLSLGLAVAIIIATVGVVMMSWPRQNMAALSWHSAALGV
                     ASGAMFAIAAVGFRGGIVALGAKNFVVGASTTLALGLVIQTLALSCYLLLFERENLIA
                     IFRAWRPSMTAGFMGAFASQMWFLAFAVESAAKVRTLGLIEILFAQAVSRQMFKQDIA
                     SREAIGIGLIILGVVVLLNT"
     gene            complement(4972..5580)
                     /gene="gstA"
                     /locus_tag="VF226_02620"
     CDS             complement(4972..5580)
                     /gene="gstA"
                     /locus_tag="VF226_02620"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX7072908.1"
                     /translation="MKLYYSPGVCSLAPHIVLRETNTPFDLVKVDLNAKKTEDGRDFW
                     QISPAGYVPALELDDGTVLTEGPAIMQYIADRADATHLAPRNGSIERYRLQSWLNFVT
                     SELHKGFGVLFNSKMPEEGKAVAREVLLARIGVVDKHLGSNAYLLGKDYTLADAYLFN
                     VISWSKPLKLDLSGFRNVSDHQSRVAARPAVKAAMKAEGLIK"
     gene            5820..6623
                     /gene="fabI"
                     /locus_tag="VF226_02625"
     CDS             5820..6623
                     /gene="fabI"
                     /locus_tag="VF226_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX7072909.1"
                     /translation="MAGKRGLVMGIANNRSIAWGIAKAAAAQGAELAVTYQGEALKKR
                     VEPLATELGSKLVLPCDVTDSGSMDAVFATLNEVWGRLDFLVHAIAFSDKNELDGRYV
                     DTSEKNFTQTMLISCYSFTALAQRAEKLMNGGGSLLTLTYYGAEKVMPHYNVMGVAKA
                     ALEASVRYLAADLGRNGIRVNAISAGPIKTLAASGIADFRYILKWNEYNSALRRTVTI
                     EDVGAAGLYLLSDLSRGVTGEIHHVDAGYHVQGMKNEDAPDITVVKNGE"
     gene            complement(6598..>7567)
                     /locus_tag="VF226_02630"
     CDS             complement(6598..>7567)
                     /locus_tag="VF226_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7072910.1"
                     /translation="LALMIGGGAMIALLPEWRWQVTGRLISGTGGVLLNVLMSKMVTD
                     WFAGKEIATAMGIFVNSWPIGIAFALFVLPWVAVSWGLAESYLLTLGMLLVGTVLLAI
                     LYRPPSAQAVAPASGIWPAGAALQAVIVAGGIWGLYNAALGMIFGFGTSLLVEKGWTA
                     AAAGYVTSLALWLIAVSAPAGGLIADRSGRHNFVMIGGFLVLAVLLVVAARTEYAIVA
                     FVALGMGGLSAGSVMSLPARVLEPRTRAIGMGLFFSVFYVAVVAAPIVAGWIATGVGT
                     SRAAFDFGAAMLVLCCTAYWLFWRLNERVRARTRMDLAITPRSSPP"
BASE COUNT         1369 a         2500 c         2373 g         1325 t
ORIGIN      
        1 gtcctgcggc gctacgacat cctgatgatc gccgacgagg tgatctgcgg gttcgcgcgc
       61 acgggcaact ggttcggctc gcagacgttc gggatccagc cggacacgat cacgatggcc
      121 aaggccatca cctccgccta catgccgctc ggcgccgtga cggtgagcga gccggtctac
      181 gaggcgatgg tggacgagag caagaagctc ggcgtcttcg cgcacggctt cacctactcc
      241 ggccaccccg tatcctgcgc cgttgcggtc aagacgctcg aaatctacga gcgcatcaac
      301 atcgttggcc acgtccagag cgtcgctccg gtgttcgaga cacgtctgaa agctcttcgc
      361 gaccatccgc tcgtcggtga gacgcgcggc gtcggcctca ttggcggcgt cgagctcgtc
      421 aaggacaagg ccaccaagga gagcttcgat gtccgccagg gcctcggccc caaggtcgtg
      481 aagctggctg aggaggaagg cctcatctgc cgcgccgttt ccggcgacac gatcgctctc
      541 tgccccccgc tggtcatcga cggcgcagag gtgaaccaga tgttcgacgc ccttggacgc
      601 gcactcgacc gcgccgagga gtgggtcaac aaggaaggat tgcggggctg aggcaggcct
      661 cgccgttctc gcccgccgtt ccagccctcc ctttgaatga aggggagggc tgtgaggccg
      721 aagcgggatc aaggggcgcg atctggcgtc tgtatgcccc cgcccgtcca ccaatccgcc
      781 cttcagcacc ttctcgcctg ggcgcgagtc ggacatactc tccacaacct tcacgcgcag
      841 cgaggtgccg gatggttgtc gggcggagca agtccaaatg cagttgcctc ctaatcagct
      901 tcgcgaccct gattgcgttc ggctcaaccg cgagccttgc tcagcagaat acgtcccgat
      961 cggaccttgg aaccgagcgg ctgcagctcc gtgtcgccag cttcgctatt tcggctgagc
     1021 cgcccgaatc ggtggaaccc caggagccgc agccgttgtg gcgccatacg ttcggctcag
     1081 agcgccgggc cccagcccgg cggcggccag tccaattcca ggcggatatc gttgtgctgc
     1141 agggcgtgac aaacctgggc gtcgtccggc agatgcttcc ggcaagtctg tatcgggtcg
     1201 ttgcctcgcg ccagattctg gagaacgtgc acgcgacgcg ggatgccccg caggtacaga
     1261 cgacggcgat cgccattcgc cgcgatgccc ggctgaggac cgttgctcac gagcatatgc
     1321 tcgagctggc tgagccgcca cctggcgcgg aaacgccgct ctcagcaggg atcgccatgc
     1381 gcgttctcgg caacaggcgt ccgttttggg tgctgtcggt ggaccttgct cagggctgct
     1441 ctggcagcac ggtgcaggat cagccgcaat gtgcggcggc gaagcggcag ctcgatctcg
     1501 tggaggcgtg gctttccgag aaagtggctg ccggcgaggc cgccattatc gccggcaggt
     1561 ttccaataga gcacggcgag ggcagtttgc cgggcaagct gggccaactg acagggtcaa
     1621 acgggaagct tgccggaacg tgccccgatg gcggcaagag cattgcgcga acctacgtgc
     1681 tgatagcgcc gagctcgcgg ccggatgcag aagtaacctt ccagggccac gtcgagcctg
     1741 tcgacaaaag gaagcccgag tggggctgcg ccctgctcgc tgacgtcagc ttctaaatcc
     1801 agcacatcag acgtgctggc cgccattgat gtgcagctcc gctccgttca cgtagctcga
     1861 ctgatccgtg cagaggaagt agatggcctt tgcgacctcc tccggctcgc cgagccgccg
     1921 cagcgggatc tgctcacgca cgatcttgtc ggtgcctggc gacagaatgg acgtgtcgat
     1981 ctctcccggc gagatggcgt tgacgcggat gccgcgcggc ccgaagtcgt gggccatctc
     2041 gcgtgtgagc gcggccagcg ctgctttgga cgtggcataa gccgcgcccg cgaacggatg
     2101 cacccgcccg ccggcaatgg aggtcacgtt cacgaccgca cccttcgcgg cttcgagctc
     2161 ggcgatcaat ccgcgggcca gcatgatcgg agcgaagaag ttgacttgaa agacggtctg
     2221 ccaatcttcc aaggccgtat cgatggtgcc aagccgcgct ccctccggtc ccttggggct
     2281 gatccccgca ttattgacga gcgcgttgag ccgcgagccc tgtgcctcga gacgcctgcg
     2341 catctcgaca atcgcccgaa gcgtgtcctc ggcattcgag aggtcgacct gaatgtgatc
     2401 ctccggcccc atctcccacg gacagttctc cgggaatggg tgacgcgagc aggtgatgac
     2461 ccgccagcct gcggaggcga accgcttgac ggtggcgtgc ccgatccccc gcgaggcgcc
     2521 ggtcaggatc agggtctttc gctcggcatt gctgacagcg ggcatggcgg tatgtcatcc
     2581 acgaatgacg agagacggtc tcggaactgc attgcagcat agccgaaatc gtctccgcgc
     2641 gtcataggtt tagcggtcgc gacaatgcag ccgccggcaa gacgcgatca gggataaagg
     2701 cgcgtcctag accacggctc gcctactgcc gcacgacgga actgcacgcg gtcatgcagc
     2761 ctgaacggcc ggtcgtgcca gaactccatc tccaccggca cgacacggaa gcccgaccag
     2821 tacggcgggc gcgggatttc cccgactgcg tacttggccg tgtagaaagc gaccgccttc
     2881 tccagtgcga agcggctttc gagcggtctc gactgagcgc tggcccaagc accgatgcgg
     2941 ctgccgcgtg cgcgggtcgc gaaataggca tcggcctcct ccggcgcaac cggcgagacg
     3001 agcccacgca cgcgcacctg ccgccggagg ctcttccagt ggaaacagag cgcggccttg
     3061 cgtgaagcca ggagctcgcg gcctttggcg ctttcgtaat tggtgaagaa cacgaaaccg
     3121 cggtccgacc gctccgggcc gtccacaccc ttgagcagca ccatccgtac gttgggcatc
     3181 ccatcggcgt cgacggtggc gagcgccatg gcgttcggat cactgggctc gtgggcggcc
     3241 gcgtccttga accactcgtc gaacagcgcg aacggctcgt ccgcggcggt gaagtctccc
     3301 gcctgcggtc ccgtgtagta ttcgggcttc ggtgtgtcgg ccatggaaat ctgctcactg
     3361 tgtcgaccgg gaaggaacag ccgttgaggt cctcttaacc cggtcgcggc attctctctc
     3421 gcgtacgagt acccgtccgg ccgcggcggg tccagtcagt tcgagtacga gtgagtcatc
     3481 atgcgtaaac gagacggcgc attcattcgc gttgcggcat gtgcgcttgc gctcgccctg
     3541 ccgatcgccg cgagtgccga tgacatcaga tcaggcgcgt cgaatcgcgg tagcgcctgc
     3601 tcctgccctg agctgcgcag gctgcccccg ccgaacgtca gcaaccccgc cgccaccttc
     3661 accctcaatg agctcgacga gatcgcgacg ctgaacgccc ttcacatcgg cctgaccgag
     3721 gtgcctgatg gcgccaactt cgtctggcgg cgttcccatg gccggctgag tgggaccgtg
     3781 cgaccgctct tctcctttaa gaatgccaag ggcgaggtct gccgccacgt cgtcgtgacc
     3841 ctccgggcag gcgatcgcac gcgcaccgtc gaaggcgctg cgtgcaggga gccgaatggc
     3901 cgctgggtgc tggagggctg agcaggtctg ccgcaacggc cctcgccaag agcgatccca
     3961 atctgctaga gcgcagtccg aagccgcgct tggcggcatc ttggctttgg gagacgcgcg
     4021 tgtcggagtg gctgtgggtc atcttcaccg tcctggcggc cggggggcag accctccgca
     4081 acgccatgca gaaggagctg acgaccacgc tcggcacggt cggcgccacc cacgtccgct
     4141 ttctgttcgg cctgccgttc ggactgcttt tcctcctgat ggtgctgctg gccacgggtg
     4201 agccaatccc caccctcagc ggctcgatgc tcacgtggac gatcgttgcc gcgctcgccc
     4261 agatctgcgc cacggccttg ctgctggccg ccatgctcga gcgctcgttc gtggtgacga
     4321 tcacactgtc gaagaccgag cccgtccagg tcgccgtttt cgggctggcg ttcctgggtg
     4381 atcatctctc actcgggctt gcagtcgcga tcatcatcgc caccgtcggc gtcgtgatga
     4441 tgtcctggcc gcgccagaac atggcggcgc tgtcctggca ctccgccgcc ctcggcgtcg
     4501 cctccggagc catgttcgcc atcgctgccg tcggcttccg cggcggcatc gtggcgctcg
     4561 gagccaagaa tttcgtcgtc ggcgcttcga cgacgctcgc gctcggcctc gtgatccaga
     4621 cgctcgcgct gtcctgttac ctgctcctgt tcgagcgcga gaacctcatc gccatcttcc
     4681 gcgcctggcg gccatccatg accgctggat tcatgggcgc cttcgcctcc cagatgtggt
     4741 tcctggcgtt cgccgtcgaa agcgccgcga aagtcaggac gcttggcctt atcgagatcc
     4801 tgtttgcgca ggcggtctcg cgccaaatgt tcaagcagga catcgcctca cgcgaggcaa
     4861 tcggcatcgg cctgatcatc ctgggggtgg tggtgctcct caatacgtaa aagcggccgg
     4921 gccgcacccg cgccccagcc gccgcaagcc cgatcgcttg agcaccaatt cttacttgat
     4981 cagcccttcc gccttcatcg cagccttgac tgccggccgc gccgcgacgc ggctctggtg
     5041 atcggagacg ttccggaagc cggagagatc gagcttgagg ggtttcgacc aactgatcac
     5101 gttgaacaag taagcatccg ccagcgtata gtccttgccc agcagataag cattgctgcc
     5161 gagatgcttg tcgacgaccc cgatgcgcgc cagcagcacc tcgcgcgcga ccgcctttcc
     5221 ctcctccggc attttcgaat tgaagagaac cccgaagcct ttgtgcagct cactggtcac
     5281 aaagttcagc caggattgca gtctgtaccg ctcgatcgag ccattgcgcg gggcgagatg
     5341 cgtcgcgtcc gccctgtcgg cgatgtactg catgatcgcc ggcccttccg tcagcaccgt
     5401 accgtcgtcg agctccagcg caggcacgta gccggcagga ctgatctgcc agaaatcccg
     5461 cccgtcctcg gtcttcttcg cgttgaggtc caccttcacc agatcgaagg gcgtattcgt
     5521 ctcgcgcagt acgatgtggg gcgcgagcga gcagacacct ggagaatagt agagcttcat
     5581 gtcaaatctc cttcattagt ccggtggttc cgttttaccg aactactgaa tgaaatttaa
     5641 gataagatct tgctcgagcg ccggtgatga cgcagatgtg aacgcgcgcg ccgcggccac
     5701 gagcggcaat ctggattctc aacgccaatg gtgcttagga tgccatggca aggaactgac
     5761 ggggataacg gctcatgacg agcaacgggg cgaatacggg agtcgctact ggaaatttga
     5821 tggccggtaa gcgcggcctc gtcatgggca tcgcgaacaa ccgctcgatc gcctggggca
     5881 tcgccaaggc cgccgctgcg caaggggcgg agctggcggt cacctatcag ggcgaggcgc
     5941 tgaaaaagcg tgtcgagccg ctggcaaccg agctcggctc caagctcgta ctgccttgcg
     6001 acgtcacgga ctcgggctcg atggacgcgg tgttcgccac cctcaatgag gtgtgggggc
     6061 gcctcgactt cctggtccac gccatcgcct tctcggacaa gaacgagctg gatggccgct
     6121 acgtcgacac ttccgagaag aactttacgc aaacgatgct catctcctgc tactccttca
     6181 cggcgctggc gcagcgcgcg gagaagctga tgaacggtgg cggctcgctt ctgacgctca
     6241 cctactacgg cgccgagaag gtgatgccgc actacaatgt catgggcgtc gccaaggcgg
     6301 cgctggaggc gagcgtgcgc tacctcgccg ccgacctcgg ccggaacggc attcgggtca
     6361 atgccatctc cgccggtccg atcaagacgc tggccgcttc cggcatcgcc gacttccgct
     6421 acatcctgaa gtggaacgag tacaattccg ccctgcggcg cacggtgacc atcgaggatg
     6481 tcggtgccgc gggcctctac ctgctctccg atctctcccg cggggtcacc ggcgagatcc
     6541 accacgttga cgccggctat cacgtccagg gcatgaagaa cgaggacgcg ccggacatca
     6601 cggtggtgaa gaacggggag tgattgcgag gtccatcctc gtcctcgccc tcacacgctc
     6661 attgagccgc cagaagagcc aataggcggt gcagcaaagc acaagcatcg ccgccccgaa
     6721 gtcgaaggcg gcgcgtgacg tgccgacgcc tgtcgcgatc caaccggcca cgataggagc
     6781 cgcaacgacg gctacataga aaacgctgaa gaacagaccc atgcctatcg ctcgagtcct
     6841 gggctcgagg acgcgagccg gcaagctcat taccgacccg gccgacagac ctcccatccc
     6901 aagtgccacg aaggcgacga tcgcgtactc cgtccttgcc gcgacgacca gcaggacagc
     6961 aaggacaaga aaccctccga tcatcacgaa gttatgccgt ccggaacgat cagcaatgag
     7021 accgccggcc ggcgctgaaa ccgcgatgag ccacaaggca aggctggtaa catagcccgc
     7081 agcagcggcc gtccagccct tctccacgag cagggacgtg ccgaagccga agatcatgcc
     7141 gagagctgcg ttgtagagac cccaaatgcc gccagcgaca atgacagcct gcagtgcagc
     7201 gccagctggc caaatccccg acgcaggcgc aaccgcctgt gcggaaggtg gccgatagag
     7261 tatggccagc agcacagtgc cgacgagcaa cattcccagg gtgaggagat agctctcagc
     7321 gagcccccag ctcacggcca cccacggcag cacgaacagc gcgaaggcaa ttccgatcgg
     7381 ccaggagttg acgaatatgc ccatggccgt tgcgatctcc tttccggcaa accagtcggt
     7441 gaccatcttc gacatcagga cgttgagcag cacgccgccc gttccggaga tcagccggcc
     7501 ggtgacctgc cagcgccact cgggcaggag cgcgatcatc gccccgccgc cgatcatcag
     7561 cgcgaga
//
LOCUS       DASVDU010000038        29505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1535046, whole genome shotgun sequence.
ACCESSION   DASVDU010000038 DASVDU010000000
VERSION     DASVDU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 29505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29505
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1535046"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF226_02635"
     CDS             complement(<1..284)
                     /locus_tag="VF226_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7072911.1"
                     /translation="MSSSAHAYAELQVTTNFSFLRGGSHPHELVAQAKALGLSALAVT
                     DRNTLAGVVRAHTSAKEAGLRLIVGARLDLQDAPSLLCLPTDRAAYGRLCR"
     gene            complement(296..1912)
                     /locus_tag="VF226_02640"
     CDS             complement(296..1912)
                     /locus_tag="VF226_02640"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX7072912.1"
                     /translation="MRRFASVWLPAWPIERMRRADPDAVPDDRPLALVEAGARGIVVV
                     AVNLLARSEGIRPGQSLADARAIFPGLLTRKIERDKDQAALVALARWSGRYGPARNSD
                     GDKGLWIDVTGVPHLFGGEHHLMRDLAGRLRAFGLTVRAGLADTTLAAAALARFAPVG
                     AEGFAIAPAGQMQGALAGLPVEALGLEDEAVLLLKRLGLRRIGQLYELPRAALARRFR
                     ELRTGRRRTKDPADLAGVVLARLDAALGQAPEARCVLSEPPRYLARRVFSEPLISAEG
                     IESAVTDLAADLCKLLDQTAHGARRLQLCLYRVDGTMAEVRTGTSKPCREPIHLLKLL
                     APKVSTIDAGFGVDVLTLEAASLEQLDPTQASLSAGLDSDARGDPTQLIDKLANRLGA
                     ERVVRLARFSSHIPEQAEVRIPILTAKELPKTRFSAQESCMRGAIRPLLLFATPEPIT
                     VLAEVPEGPPARFTWRRVSHTVAKAEGPERIEPEWWRTIGNSAKPPRPRDYYRIEDDR
                     GGRYWVFREGLYDRTPEAGPPVWYMHGLFG"
     gene            complement(1830..2714)
                     /locus_tag="VF226_02645"
     CDS             complement(1830..2714)
                     /locus_tag="VF226_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072913.1"
                     /translation="MVDDLRQRIRRIERDGGVTWQEPALPPASPASHAGLGAGATSSQ
                     RTEGNGAGPVWLLGAGEVDALLGPDGLEIGGVHEIKPALREPGVSWAATWAAAFAFSL
                     ALGVRRSAMHNGRAESPILCCRSAALANELGGLYGLGLRTLGLAPDRLIIVETANAAD
                     TLWAMEEGLRSESLALVLGLLHTVPLTPARRLALAAAAHRSPCLLLTHPRTEVTAATA
                     TRWRVGPAPSAPHPFDPRAPGAPRFTVDLERCRAQPISAATASFSLEWCDEALCFRLA
                     SGMADRADAPRRSGRGAR"
     gene            3171..3818
                     /locus_tag="VF226_02650"
     CDS             3171..3818
                     /locus_tag="VF226_02650"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7072914.1"
                     /translation="MVLRKIMSRVVVAALAGLLPAAAVAAPGYQSNSLWGGTPEWGGE
                     RQIVPFSTKYNPGEIVVSFGDRRLYLIESRGKAISYPIAVPREDSRWQGTTNVSMKRE
                     NPSWTPTPSMLRANPKLPPWVPGGHPMNPLGVRALYLGSSLYRIHGTDAPWTIGQAVS
                     AGCIRMYNKDVLDLYPRVRVGTKVTVTWQRFTTTAGADPVREHKTAGWFPWKATR"
     gene            complement(3888..4805)
                     /locus_tag="VF226_02655"
     CDS             complement(3888..4805)
                     /locus_tag="VF226_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7072915.1"
                     /translation="MLLLVLPGALRAEVRVVATSKPIHALVSSVMAGIGTPELLVGGQ
                     TSPHSYSMKPSDAQKVHNADVFFRVSEQLEPFTRKLVQSLPGSVRVVSLIDAPGLTLL
                     EMRRGGVFDGHEAHGPEIDEEPGLEHGHEGPHDPHIWLDPENAKAMVEYIAEVLATIA
                     PDQATTFATNAQAELRRLTDLSEELARELAPVVGKPFVVYHDAFQYFENRFGLAAVGS
                     VTVNPDLPPSGKRLQELRSLIRSAGARCVFAEPYLPSRAIEVIAEGTGAKTGILDPEG
                     LTLQPGPQLYAELMRNLSRSFRACLLSSP"
     gene            complement(5051..6580)
                     /locus_tag="VF226_02660"
     CDS             complement(5051..6580)
                     /locus_tag="VF226_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX7072916.1"
                     /translation="MYGRISTLAFVGIEARPVQVEVRISPGQHVFAIVGLPDKAVAES
                     RERVRNALHAIGLGIPWTHITVNLAPADLPKEGSHFDLPIALALMVAMEAISPDAIEG
                     FAAIGELALDGQIRATPGALPAAIGANAMGKGLICAAASGPEAAWAAADMDIIAAPHL
                     LSLVNHFKGLQTLRRPEPNLAQDDQALPDLADIKGQETARRALEVAAAGGHNMLMIGP
                     PGSGKSMLASRLPSILPPLDPQEMLEVSMVHSLAGELLGGKLRTQRPFRAPHHSASMA
                     ALVGGGTRPKPGEVSLAHLGVLFLDELPEFSPQVLDSLRQPLETGETVIARANHRITY
                     PSRIQLIAAMNPCRCGGGAPGQTCKRGPRCSAEYQARISGPLLDRIDLQIEVSAVTAA
                     DLVLPAPAESSAVVRARVTAARERARERFAKLGAAGVRTNAQATGRILEEIARPDADG
                     IALLRQAAETLQLSARGFHRTLRVARTLADLDGADVVGRIHIAEALSYRGEAIRQRQA
                     A"
     gene            6747..7745
                     /locus_tag="VF226_02665"
     CDS             6747..7745
                     /locus_tag="VF226_02665"
                     /inference="COORDINATES: protein motif:HMM:NF004792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7072917.1"
                     /translation="MARIAGASIVVTGASSGVGRATALALADHGARLTLAARGREALE
                     RVASECTERGGPAVAVPTDISDSDATVKLAEAAASTFGGLDAWINVVGVGAFGRFEET
                     PIEAHRRVIETNLIGAVNGAHAAIPYFRAQGQGILINVNSLGAWVPSPYSAAYAAGKW
                     GLLGFSKSLRAELSRSRGIAVCDVFPSFLNTPGLDHAANYTGRELAAAPPVYEPEAVA
                     NAIVNLIQRPRSEVTVGVPAKLAWLLGTMAPGLAGWLAGRAIAVYLNRAGTVPITDGN
                     LFESGGGTNRVHGRQTAQNRKLLGAGLGTVALAAALGMAPRLLRGAPSATPGRAGS"
     gene            complement(7754..8701)
                     /gene="gshB"
                     /locus_tag="VF226_02670"
     CDS             complement(7754..8701)
                     /gene="gshB"
                     /locus_tag="VF226_02670"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX7072918.1"
                     /translation="MPMKIACQMDPIDRIDIRGDSTFALLLEAQRRGHELFYYEPPNL
                     ALDGARLIARGAALRVEDRVGDHYKLSDRRLVDLAEWDVVLLRQDPPFDMAYITTTHL
                     LERIHPKTLVVNDPTWVRNAPEKVWVLDFQDLMPPTLVTRNLDDVKRFRERHKDIILK
                     PLYGNGGSSVFRIPEGDTNLNSLVELFQTVFREPFMVQQYRPEIRKGDKRIILIDGEA
                     VGAINRVPAPDETRSNMHVGGRAMPTELTERDLEICERLKPELKRRGLIFTGIDVIGP
                     YLTEINVTSPTGIRQIKDFGGPDIAALFWDAVEARTRRA"
     gene            complement(8748..9107)
                     /locus_tag="VF226_02675"
     CDS             complement(8748..9107)
                     /locus_tag="VF226_02675"
                     /inference="COORDINATES: protein motif:HMM:NF009151.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX7072919.1"
                     /translation="MNIRRQREQSGRLAEFVAALLLCLKGYRILARRYRSKLGEIDLI
                     AVRGRRLAFVEVKRRPTMEAAEAAKTIRQARRIARAAEQWVWRHPAYRDYEIGLDAML
                     IVPGHLPRHQPNALQLT"
     gene            complement(9140..10078)
                     /gene="rsmI"
                     /locus_tag="VF226_02680"
     CDS             complement(9140..10078)
                     /gene="rsmI"
                     /locus_tag="VF226_02680"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX7072920.1"
                     /translation="MAKNRRISKEESGDVSVESLRRHVLARLDDALAQPLGPGLYLVA
                     TPIGNLADFTLRAISTIARADAVYCEDTRHSRQLLVQYGIDRDLRSFHEHNEDRELPR
                     VLEEIAAGRAVALISDAGTPLLSDPGFKLVRSAVAAGYRVVSIPGPAAPLAALTSCGL
                     PTDRFLFAGFLPTKQAARRARLAELASIKATLIFFEAPSRVEAALADMAAMLGSEREA
                     AIARELTKIHEEVVRGRLGDLAKKSSGEDMRGEFVLLVGPPAPTEVSDEAIAARLEQS
                     LAESSVRDASREVAEALGVPKSRVYDLAVRMTRGRT"
     gene            10257..11486
                     /locus_tag="VF226_02685"
     CDS             10257..11486
                     /locus_tag="VF226_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEX7072921.1"
                     /translation="MAFHVAWVRLGSALALAVLAAILAGCAIQGGGASLSPSGDGAVD
                     PQDSAARPSVKVALLLPLTSTGHTEAVAKAMKQAAELAIFELDNPNVQLIAKDDKGTP
                     EGAQAAAEEAIREGAGIILGPLFAGSVRAVAPVAGQAGIPVLAFSNDSQVAGNGVYLL
                     GFPPQQEVERVIGYALSRGHRRFAALLPEDAYGKITESAFRQAVGAGGGAIAALETYP
                     PASNAMVEPAERIVAALRQAEEAQQPITALFIPGGPEVLPRLGPLLSYANIDTAQVKL
                     LGTGGWDFPNIGRERVFVGGWYASADPRGWRDFSERFANTYGSSPPRLASMAYDAVSI
                     VVRLSSEPAGTRFSAASLTRPQGFTGIDGPVRLLPSGIAERGLAVLEVQDIGATVIDP
                     PRGFSSAGSSSLSSGFN"
     gene            11617..12561
                     /locus_tag="VF226_02690"
     CDS             11617..12561
                     /locus_tag="VF226_02690"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7072922.1"
                     /translation="MLAILTIVIPVFGVMFAGWVTERWRFLPEGSAKTIALFSFNVAM
                     PAFLFRAMQEIGEVPEAPWKFVVAYFGGIVIVWVAAALVARTVLHRSAADGAAISMGS
                     CFGNTVMLGIPLALTAFGPDAAAPIGILVSLDSLVLWVIATLHIEWANRQARTTPFFR
                     AVGGVVLDLVRNPVVMGIVVGSVWRIAGLGVPELADRFLALIGQAAVPSGLFALGMSL
                     ATYRIAGQMPTLATIFAMKMLLLPVVVYFLAGWVFNLTPVWVAVATLFAAMPVGANAF
                     LFAIKYDRAVGSVSAAIAVSTVVAVGTASAVLYMMGIG"
     gene            complement(12679..13302)
                     /locus_tag="VF226_02695"
     CDS             complement(12679..13302)
                     /locus_tag="VF226_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008508073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7072923.1"
                     /translation="MLDRRQFFAASLFAATALGAAPRFSNSLRAQGFPSEEDVYFDPD
                     IPVLGNPNGDVTIAEFFDYQCPYCKKNHPELKKVVASDRKIRLVMKDFPIFGKPSVYA
                     ANLVLASAADGKYATAHDALMNTPGRLSREDVDAVLKKAGLDPTDLYSKYKKNRSHID
                     GIILRNMAQANAFNLQGTPAFVIGRKIYPGYMNEKALKEAVAEARKS"
     gene            complement(13304..14149)
                     /locus_tag="VF226_02700"
     CDS             complement(13304..14149)
                     /locus_tag="VF226_02700"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7072924.1"
                     /translation="MRKVHCRRQGRLLAFLLSSGASTLGKPLRKDCNRNSKIRTSTDK
                     EASLYILSSGFKTTLRVALLASAAIAASGCTATTRSLVGVAEASQEGPGSVHSNPYGE
                     VNDNGTIIPAVDTSKTNPRYLRQIVDYQTDEPVGTIIVDPQNRFLYLVMEGGKAMRYG
                     VGVAKAGLEFEGEAIIERKAAWPGWAPTADMIKREPERYGPYANGLEGGLHNPLGARA
                     LYLYKDGKDTLYRIHGTNEPWSIGKAVSSGCIRLMNQDIIDLYKRVPKGARVVVLGAK
                     QKGES"
     gene            complement(14181..15962)
                     /gene="dsbD"
                     /locus_tag="VF226_02705"
     CDS             complement(14181..15962)
                     /gene="dsbD"
                     /locus_tag="VF226_02705"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEX7072925.1"
                     /translation="MRFVILGLVLLGIFAAPAAADPLPADQAFQIRASRTTDGKVRVD
                     WTIADGYYLYRAHIAAKGVDGSDLKVETPQGSIKDDPVFGATEVYYRRASATVVAASE
                     EPIEITYQGCQEGGICYVPQTKRVNPLTLEVATNSDSGNVPSPGIAASVGAAEKPAAA
                     FRLAQEEGLVQSLLRKGGLPLVVFSFLGFGLLIAFTPCVFPIYPILAATLAREGDRLT
                     ARRGFVLSAIYVLSSASAFSLLGALAGWSGQNLQIVLQSPFTTGAISIAFILLALASF
                     GFFEPRLPAGWVNWIAARTGKLKVSKASVAVLGFSSVFIIGPCVTAPLAAALLYLTQS
                     GDMLLGAAALFALGIGKGIPLIVVGTMGASILPRAGAWMKHVKTSFGIGFLATAIWIA
                     TPLLPAGLDLVLWAALLVGTGMWAFRFATGNRGFILLARTTGLMAITYGMILMIGAAT
                     APTGPWQHLARLTNSAPPSTDGDLHFATVTSVPELQKELRSVSGRKPTLVYFTADWCI
                     TCRVIERSVLPHDEVRRSLEGLHLVKVDISALHKDSPELMQRLRVVGPPTMVFFDRDA
                     REVAGTRLVGEVTAELLSRSAAKVQSF"
     gene            16094..16759
                     /locus_tag="VF226_02710"
     CDS             16094..16759
                     /locus_tag="VF226_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7072926.1"
                     /translation="MRILIVEDDPLLLDGLAIGLGLKGFTVDAVANCEDGRIAVRMHS
                     YDCIVLDVMLPDGSGLDLLKELRAAKNCTPVLLLTARDQVPERIAGLDAGGDDYVGKP
                     FDLDELAARLRALARRGAGRATAYLEWRGVVLDPAALRATKDGVSLKLTRREFAVLRA
                     LMERPTAIVSKAALEEALYGWQEEIESNAVEVHVHHLRAKLGQDFIKTVRGLGYRLAE
                     EGA"
     gene            16756..18105
                     /locus_tag="VF226_02715"
     CDS             16756..18105
                     /locus_tag="VF226_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7072927.1"
                     /translation="MNSIRGRLLAILIGTTLFVWIAAITWIYTSTQAEVERVLDARLT
                     EAARMVNSLLADHRIPIRDDATSSKPAIRAATKVYERQLSCQIWSLDGTLVARSESAP
                     GLPLTKAMEGFSEDIVDGETWRVFTIENPELGVRVMVGDTLRMRDRLVGDVVKGMVHS
                     AAVAIPFLVGLIWLSVSHGLIPLSDLACALSRRSANELSPLPSAGIPKEIAPAVQAIN
                     ELFGRVEAARERERSFTAFAAHELKTPLAGLKTQAQVALSSTDAAVHANALQQIVAGV
                     DRTSRLVNQLLDMAALEASDVQPCSSDESLNHLVNSVVADLLSLASTRGIAIEVDSSK
                     DDVRAKVEPQFFILALRNLLENALDHAKSVVRCQALDQGQWVRVVVEDDGPGILEPEL
                     ERVTAKFVRGRNKSMTGSGLGLAIAKIAMARIDGKLTLHNREPTGLVAMLEIPRQAA"
     gene            complement(18227..18303)
                     /locus_tag="VF226_02720"
     tRNA            complement(18227..18303)
                     /locus_tag="VF226_02720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18267..18269),aa:Arg,seq:cct)
     gene            18450..19280
                     /locus_tag="VF226_02725"
     CDS             18450..19280
                     /locus_tag="VF226_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX7072928.1"
                     /translation="MAKGAVLGIRSAIRALILAGLLVGPVAAAERPGCKLESGPARGV
                     AKVIDGETLELDDKSQVRLLGALAPQALDGGADEGAWPPADAAKTELEKLALGKSVEL
                     RFASRRSDRYGRLLAHVFVREGSEHTWLQEEMLRRGYARAYALPGSTECLQDLVAAED
                     HARRSGLGIWGHASYQIRPADRHWELLRYRSTYQLVEGKVRDAAEVRGRIYLNFGENW
                     REDFTITVQPTNKRVFDEAGMKLTDLKGRRVRVRGWIERRGGPLIEIYHPSQLEVLPE
                     "
     gene            complement(19434..19952)
                     /gene="rimM"
                     /locus_tag="VF226_02730"
     CDS             complement(19434..19952)
                     /gene="rimM"
                     /locus_tag="VF226_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599664.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7072929.1"
                     /translation="MGVKSGRILLGRIGPAHGIRGHVLVQTYTEAPEDIASYGALEDE
                     SGRRQFEIEIVRVTPKGVVARIAGVEDRNAAEALRGTELYVSRERLPPTQEDEFYLSD
                     LIGLPAFTPDGTRLGEVVAVHNFGAGDILEIRLGDGRNTEMVPFTKDFVPEVDLSSRR
                     MTIDMPAEGEEE"
     gene            complement(19994..20356)
                     /gene="rpsP"
                     /locus_tag="VF226_02735"
     CDS             complement(19994..20356)
                     /gene="rpsP"
                     /locus_tag="VF226_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7072930.1"
                     /translation="MAVKLRLARGGAKKRPYYYIVAANSTAPRDGRYIEEVGKYNPML
                     KRDDPNRVKLDIERCKHWLSVGAQPTDRVARFLDAAGLIKRAPKHNPIKGKPGKKRLE
                     REAAEAEKAKEAAEAGQA"
     gene            complement(20811..22331)
                     /gene="ffh"
                     /locus_tag="VF226_02740"
     CDS             complement(20811..22331)
                     /gene="ffh"
                     /locus_tag="VF226_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7072931.1"
                     /translation="MFQSLSDRLSGIFDKLTKRGALTDSDVSEAMREVRRALLEADVA
                     LEVVRDFTEKVKAKAVGQDVVKSVTPGQMVVKIVHDELVRMLGSDAQPIDVAATPPVP
                     ILMVGLQGSGKTTTSAKIAYRLKNRDKKRVLMASLDTRRPAAQEQLRVLGEQIEVPTL
                     PIIAGQTPLQIAHRALDAARLGGYDVVILDTAGRVTVDEALMLEVAEVEAATNPHEVL
                     LVADALTGQDAVNTAKAFEGRLGLTGIVLTRIDGDGRGGAALSMRAVTGKPIKLLGTG
                     EKWDALEDFHPERIASRILGMGDIVSLVEKAAQTIDAEKAQKIAQKMKKGDFDLEDLA
                     EQIKQMQKIGGMSGVLGMLPGIGKIRNQIAEANLDDKLLKRQHAIITSMTPKERRNPK
                     IIDGKRKRRIAAGSGTKVEEVNRLLKMHRQMADMMKAMGKNKGMLARMFGGGGMPQPT
                     PEMVEQLQRGEMPKGLPPMPPGGVPRLPGLGGGLPGLGGGSAPKFPGLPGFPAKKK"
     gene            complement(22362..23006)
                     /locus_tag="VF226_02745"
     CDS             complement(22362..23006)
                     /locus_tag="VF226_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7072932.1"
                     /translation="MLKVWGRKTSINVQKVMWAVGELGLPHQRIDAGGPFGGLDTPEF
                     GKLNPNRLVPVIEDDGFVLWESNAIVRYLTAVHGQGKLAATDERQRALADQWMEWANS
                     MLYADIILTCFLGLIRTPAADRNAGAIEAAAKRAGERLAILDAQLEGRSYILGDQPTA
                     ADIGCGSLMYRYFTLDIPRPRLGNVEAWYQRLTERKAYQDHVMIDYQAMKVPGA"
     gene            complement(23288..24421)
                     /locus_tag="VF226_02750"
     CDS             complement(23288..24421)
                     /locus_tag="VF226_02750"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7072933.1"
                     /translation="MRMSIATLATFAFMTPAAAVQTKAPAAPESRVTVETVAEGLVHP
                     WAIQFLPGHRLLVTELPGRMRIVNRDGRLSEPIANVPRVFAEGQGGLLDVALAPDFED
                     SRIIYFSFSEPREGGGNGTSVARARLAESDGKARLEELRVIFRQLPPFNSNVHFGSRI
                     VIAPDGKLFVTLGERFQMKYAQDLTRDWGKVVRINPDGSAPDDNPFVDRENARPEIWS
                     YGHRNPQGAALHPETAELWIADHGPRGGDEVNVVRKGRNYGWPVINYGRHYSGQDIPE
                     RREGMEQPLYYWDPSIAPSGMAFYTADRAPQWKGNLFVGALAGRHLSRLVLDGEQVTA
                     EEKLLTDLGERIRDVRQGPDGALYVATDSARGRILRVMPQNQD"
     gene            complement(24474..25046)
                     /locus_tag="VF226_02755"
     CDS             complement(24474..25046)
                     /locus_tag="VF226_02755"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7072934.1"
                     /translation="MPKPSTSSEMRPASEWVVRFLHGIPRGGQVLDVACGSGRHLRLG
                     LAHGYKVVGIDRDLAGVADLKGQPGVELIEADLEIGNPFPLRGRTFAGVIVTNYLWRP
                     ILPDIVTCVAPDGVLIYETFALGQERMGRPANPEFLLNPGELVDAVRDRLVPIAYEHT
                     TLSEPRRIVQRIAAVGHGHPWLKEPPRQPT"
     gene            25093..25962
                     /gene="dapF"
                     /locus_tag="VF226_02760"
     CDS             25093..25962
                     /gene="dapF"
                     /locus_tag="VF226_02760"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX7072935.1"
                     /translation="MTSGDAIPFKKMNGLGNEIVMVDLRGCARVFSEEEARAIAADPR
                     THFDQLMVLHDPRSPGTDAFVRIYNTDGSAAGACGNGTRCIAWAVAQETGRRDFRFET
                     SSGVLQTHVESIDDITVDMGEPRFRWDEIPLAEPFHDTRRIELQIGPIDNPVLHSPSV
                     VNVGNPHAVFWVDDVDAYDLGRFGPLLETHPIFPERANISLAQVTSPSALKVRTWERG
                     AGLTKACGSAACAAAVCAARTGRTGRMVTVTLPGGMLHIEWTAANRILMRGPARLEYE
                     GLLDPELFVKTPA"
     gene            26096..27355
                     /gene="mtaB"
                     /locus_tag="VF226_02765"
     CDS             26096..27355
                     /gene="mtaB"
                     /locus_tag="VF226_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEX7072936.1"
                     /translation="MSDPTVITLGCRLNAYESELMRRHAAEAGLEDAVIINTCAVTAE
                     AVRQSQQAIRRARRERPDAKVIVTGCAAQVDPDLFAGMAEVDHVIGNAEKVKRETFLG
                     LNLANSERVQVNDIMSVRETASHLIDGFGTRARAYVQVQNGCDHRCTFCIIPYGRGPS
                     RSVPAGEVVAQVRRLVEAGYLEVVLTGVDITSYGNDLPGRMSLGRLVRQILRHVPELK
                     RLRLSSIDQVEADKDLMDAIAEEQRLMPHLHLSLQAGDDLILKRMKRRHLRSDAIRFC
                     ETARRLRPDVVFGADLIAGFPTETEQHFENTLRLVEECGLTYLHVFPFSPRKGTPAAR
                     MPQVDRKEVKERAARLRSLGSKVLARFLEQQHGSVVEVLTEGANVGRTAQFAEVKLSD
                     EVPAGALLSARIQGDDGQRLAGEVLAG"
     gene            27352..28551
                     /gene="ftsY"
                     /locus_tag="VF226_02770"
     CDS             27352..28551
                     /gene="ftsY"
                     /locus_tag="VF226_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7072937.1"
                     /translation="MSMKKPKKGGLLDRLFGRGEKTAEDGEADVRLKEPISPSEAEGP
                     QTSEEQLESSETTETVPPADAMTAEAEASFEQAPREEAGAEAAPETLEPPTKTSWFSR
                     LKAGLTKTSSRLSEGITGVFAKRKLDADTLDELEDLLIQADLGLETAARITDALSQGR
                     YDKDISPDEVRAVLASEIAKVLEPVAQPLEIKADAKPHVILVVGVNGTGKTTTIGKLA
                     AKYTRSGKKVMLAAGDTFRAAAIDQLKVWGERTGATVVARDVGADAAGLAFDAFTQAR
                     KEGFDVLLIDTAGRLQNKQALMDELGKVTRVLKKIDPAVPHDTLLVLDATTGQNALDQ
                     VQVFQKQADVSGLVMTKLDGTARGGILVAIAAKYRLPVHAIGVGEGIDDLQAFDAGEF
                     ARAIALA"
     gene            28595..29293
                     /locus_tag="VF226_02775"
     CDS             28595..29293
                     /locus_tag="VF226_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HEX7072938.1"
                     /translation="MSWLRRTYPFNAEQTVNILSEFGPLVTMFIVNAMYGISAGTWAL
                     IITTGIAIVTMRKVLGRFPAFPIIASSVTITFGFLTLFTGDAMWIQIKVTIFNLLFAI
                     FLFGGLWMGKNFFQYVFESTFHYTKKGWDRFTFSFACFFLLTAILNEIVRLTFDDHTI
                     YSLLGYQMDGVNVWILFKVAFIMPVSGLYAWWLTRLMQKYRIRTDSGSVPGDALASAA
                     ERIGRKQRPATQKA"
BASE COUNT         5307 a         9047 c         9621 g         5530 t
ORIGIN      
        1 cggcagaggc ggccgtaggc ggcccgatca gtcggcaggc agagcaggct cggcgcatcc
       61 tggaggtcga ggcgggcgcc gacgatcagc ctcagcccgg cctccttggc ggaagtatgg
      121 gcccgcacaa cgccggccag cgtgttgcgg tcggtcacgg ccagcgcgga aaggccgagc
      181 gctttggctt gggcgaccag ctcgtgggga tgcgagccgc cacgcaggaa cgagaaattg
      241 gtggtgacct gcagctcggc gtaggcgtgt gcggaggaag acatgccggc gctcctcacc
      301 cgaacagccc gtgcatgtac cagacgggag gtccggcctc gggggtgcgg tcgtagagac
      361 cctcccggaa cacccagtag cgaccgccgc ggtcgtcctc gatgcgataa tagtctcgcg
      421 ggcggggcgg cttggcggag tttccgatgg tccgccacca ttcgggctcg atgcgctccg
      481 gtccctcggc cttggcgacg gtgtggctga cgcgccgcca tgtgaagcgc gctggcgggc
      541 cttccggcac ctcggcaaga acggtgatcg gctcgggggt tgcaaacaaa agcagtggac
      601 ggatggcgcc gcgcatacag gactcttgtg ccgaaaaacg agttttcggg agctcttttg
      661 cggtcaatat cggaatgcgg acctcggcct gctcgggaat atggctgctg aaacgggcaa
      721 ggcgcaccac gcgctctgct ccgaggcgat tggcgagctt atcgatgagc tgtgtgggat
      781 cgccacgggc atcagaatcc aacccggcac tcagagatgc ctgcgtagga tcgagctgtt
      841 cgaggcttgc agcctcgagt gtcagcacgt cgacgccgaa cccggcatca atggtcgaga
      901 cctttggggc caggagcttg aggagatgga taggctcacg gcagggcttg ctggtgccgg
      961 ttcggacctc ggccatggtt ccgtcaacgc gatagaggca aagctgcagg cggcgtgcac
     1021 catgggcagt ctggtcgagc aatttgcata ggtctgcggc gagatccgta accgcactct
     1081 cgattccttc cgctgaaatg agtggctcgg agaaaacgcg tcgggcaagg taccgagggg
     1141 gctcggaaag cacgcatctg gcctccggcg cctgcccgag agcagcatcg agacgggcca
     1201 gcaccacgcc cgcaaggtct gccggatctt tcgtccggcg gcggcccgtc cgaagctcgc
     1261 ggaagcggcg ggccagtgcg gcacgcggaa gctcatagag ctggccgatg cggcgcaagc
     1321 cgagccgttt cagaagcagc acggcctcgt cctccagacc gagcgcttct acgggcagac
     1381 cggcgagtgc gccctgcatc tggccggcgg gcgcaatggc gaagccctcc gcgccgaccg
     1441 gggcgaaccg ggccagcgcc gcggccgcga gcgtcgtatc ggcgaggccg gcgcggaccg
     1501 tcagtccgaa ggcgcggagg cgacccgcaa gatcgcgcat cagatggtgc tcgccgccaa
     1561 agagatgagg cacgccggtg acgtcgatcc agagaccctt gtctccatcg ctgttccggg
     1621 ccggaccgta gcgaccactc cagcgggcaa gggcgacaag ggcggcctgg tctttgtcgc
     1681 gctcgatctt gcgcgtcagc agccctggaa agatggctct ggcatcggcg agggactgac
     1741 ccgggcgaat gccttcacta cgtgccagca ggtttacggc cacgacgacg atgccccgtg
     1801 cgcccgcttc gaccagtgcg agcgggcggt catcgggcac cgcgtccgga tctgcgcggc
     1861 gcatccgctc gatcggccat gccggaagcc agacggaagc aaagcgcctc atcacaccac
     1921 tccaaactga aagaggctgt cgcggctgaa atcggctgcg cccggcagcg ttcgaggtcg
     1981 accgtgaacc ggggtgcacc cggggcgcgt ggatcgaagg gatgcggcgc actcggcgcc
     2041 ggcccgaccc gccagcgcgt cgccgttgcg gccgtcacct cggtgcgggg atgcgtgagg
     2101 aggagacaag gcgagcggtg cgcggcagcc gccagcgcca gacgccgggc cggcgtgagc
     2161 gggaccgtat gaagcagccc tagaacgagg gctaggcttt ccgacctcag cccctcctcc
     2221 atcgcccaca acgtgtccgc cgcattggct gtctcgacga tgatgagacg gtcgggcgca
     2281 aggccgagcg tcctgagccc caagccgtaa aggccgccaa gctcattggc cagggcggca
     2341 gagcggcagc acaggatcgg gctttccgcc ctgccgttat gcatcgcact ccggcggaca
     2401 ccgagagcca aggagaaggc aaatgcggcg gcccaggtcg cggcccaact cacgcccggc
     2461 tcacgaaggg caggttttat ttcatgcacg ccgccgattt cgaggccatc agggccgagc
     2521 agggcatcga cctcacccgc tccgagcagc cagaccgggc cggctccatt gccttcggtc
     2581 cgctgtgaag aggtggcgcc ggctccaagg cctgcatggc tggcaggact ggcaggcggc
     2641 aacgccggct cctgccatgt cacgccgccg tcgcgctcga tcctgcggat ccgctgccgc
     2701 agatcatcga ccatactctt gcgcaggcga gcagccgtct gccccgggtc gcttggccgc
     2761 ggggaggccg caagcctctt gggccctgga tcaggaaaaa tccgatgttt gtcagtggct
     2821 tgggccataa tggcggctgc ggtgtcattt ctatgttcct gttatgttct agaaaatgca
     2881 cctgcatgtc aaccccgtcg tgaggccgct cgtgctcagc ggcgacaccc ttttcagaaa
     2941 cgatccaggc ccgttgacgg ctggtactga ttcggaactc ctcagtcgtc cgcccgttat
     3001 gaagtcagtc cacctcgcag aatcttgacc agactgtggc tttcatgtgc cggcattttg
     3061 aacgagtttg cggtatagaa acccttcatc ttgcttttca tcaacaggtg cacccggtgg
     3121 agcgcgcggc ccatcggacc tgcaaacgtg aaccaacagc ggagtacagg atggttctac
     3181 gaaagatcat gagtcgggtt gtcgttgcag cacttgccgg cctcctcccc gccgcagccg
     3241 ttgcagctcc tggctatcag tccaattcgc tttggggcgg cacccccgaa tggggcggag
     3301 agcgacagat cgtcccgttc agtacgaagt acaatccggg agagatcgtg gtcagcttcg
     3361 gtgatcggcg cctttacctg atcgaaagcc ggggcaaggc gatcagctat ccgatcgcgg
     3421 tgccgcgaga ggacagccgc tggcagggta cgaccaacgt gtcgatgaag cgggagaacc
     3481 cgtcctggac cccgacgccg tcgatgctgc gcgccaatcc gaagctgccc ccctgggtcc
     3541 ccggtggcca tcctatgaat ccgcttggcg tgcgggcgct ctatctcggg tccagcctct
     3601 acaggatcca cggcacggat gcgccgtgga ccattggtca ggcggtctcg gcgggttgca
     3661 ttcggatgta caataaggac gtgctcgacc tttacccgcg cgtgcgggtc ggcaccaagg
     3721 ttactgtcac gtggcagcga ttcacgacga cggctggcgc cgacccggtc agggaacaca
     3781 agaccgctgg ctggttcccc tggaaggcca cgcgatagat tgatcgctga catcatgtcg
     3841 ttgaaggggc ggccccggag ccgccctttt gtgttggttc cgatgtccta tgggctgctg
     3901 aggagacagg ctcgaaagct gcgggataga ttacgcatca gctccgcgta gagttgtggg
     3961 cccggctgga gcgtaagtcc ctccgggtcc aggatgccgg tcttggctcc cgtgccttcg
     4021 gcgatgacct cgatggcgcg cgacggaagg tagggttcgg caaagacaca gcgggctccg
     4081 gctgacctga tcaatgaccg cagctcctgc agccgctttc cgcttggtgg gagatccggg
     4141 ttgacggtca ccgagccgac ggctgcgagc ccgaaccggt tctcgaagta ttgaaaggca
     4201 tcgtggtaga cgacgaaggg cttgccgacg acgggcgcaa gctctcgggc tagctcctcg
     4261 gaaagatcgg tcaaacggcg tagctcggcc tgcgcattgg tcgcgaaggt ggtcgcctgg
     4321 tccggtgcga tggtggcgag cacctcggca atgtactcaa ccatggcctt ggcgttctcc
     4381 ggatcaagcc agatatgcgg atcatgcggc ccttcgtgcc cgtgctcgag accgggttct
     4441 tcgtcaatct ctgggccatg cgcttcgtgt ccgtcgaata cacctcctct gcgcatctcg
     4501 agcaaagtca ggccgggtgc atcgatgagg ctcaccacgc gcacggagcc cggcagggat
     4561 tgcaccagct tgcgcgtgaa gggctccagt tgctccgaga cgcgaaagaa aacgtcagcg
     4621 ttgtgcacct tctgcgcatc ggagggcttc atggaatagg agtgcggcga ggtctgcccg
     4681 ccgaccagca gctcgggcgt gccgatgccc gccatgacgc tggaaaccaa ggcgtgaatg
     4741 ggcttgctcg tcgcgaccac gcggacttcg gcccgcagag caccaggtag gaccaggaga
     4801 agcacggcgg gaaagatcca taacattcgt aacgctgatc gaatgacatc acccattgct
     4861 cgaccacctt tgatgcaaac ctcggcccgg cgaagctcgg tatgggaatg ttataacata
     4921 tcacttccaa ttgcgaagcc tagcgatggc ctatcgatgc tggcgtcggg gatttcgtgg
     4981 acgtccaatt ctgcgcaagt cgcggcgagc caaccttcgc gcttctctag cccagaaaca
     5041 gctcccgcgg ctaggccgct tggcgttgcc ggatcgcctc gccccggtag ctcagcgcct
     5101 cggcgatgtg gatgcggccg acgacgtctg caccatccag gtcggcgagg gtgcgcgcaa
     5161 cgcggagagt acgatggaat ccgcgggccg aaagctgtag cgtttcagcg gcttgcctga
     5221 gcaaggcgat gccgtcggcg tcggggcggg cgatttcttc gagaatacgc cccgttgcct
     5281 gggcgttcgt gcgaacgccg gcggcaccga gttttgcgaa gcgttctcgt gcccgctccc
     5341 gggctgccgt cacacgagcc cgcacgacgg cactgctttc cgccggcgcc ggaaggacca
     5401 gatcggcggc ggtaaccgca gaaacctcga tctgcaagtc gatccggtcg agcagcggtc
     5461 ccgaaatacg agcttgatac tcggctgaac accgtgggcc gcgtttgcac gtctgacccg
     5521 gggcgccacc cccgcatcgg caggggttca tggcggcgat aagctggata cgtgagggat
     5581 acgtgatgcg gtgattggcg cgcgcgatca cggtttcgcc ggtctccagc ggctggcgca
     5641 acgaatccag cacttgcggt gaaaactccg ggagctcgtc aagaaaaagc acgccgaggt
     5701 gcgccagcga aacctcacct ggtttcggcc gcgtgccgcc accgaccagg gcggccatgc
     5761 tcgccgagtg gtgcggcgcc cggaacggcc gctgggtccg cagcttgcct cccaggagct
     5821 ccccggcgag cgaatgaacc atgctgactt ccagcatctc ctgtgggtcg aggggcggca
     5881 gaatggaggg aagccgcgag gcaagcatcg actttccgga gccgggcggc ccgatcatca
     5941 acatgttgtg cccgcccgca gcggcaactt ccaaggcccg gcgcgctgtt tcctggccct
     6001 tgatgtctgc gagatccgga agggcctggt cgtcctgggc aaggttcggc tcgggccgcc
     6061 gcaatgtctg aaggcccttg aagtgattga ccagtgacag caggtgcggt gcggcgatga
     6121 tgtccatgtc ggcagcggcc caggccgctt ccggtccgct cgccgcggcg cagatcagac
     6181 ccttgcccat ggcattggcg ccgatggctg cgggcaaggc gccgggtgtc gcgcggatct
     6241 gtccgtcgag cgcgagctcg ccaatcgcgg caaatccctc gatggcgtcg ggcgaaatgg
     6301 cctccatggc gaccatcagc gccagcgcga tcggcaggtc gaagtgactg ccttccttcg
     6361 ggaggtcggc cggggcgagg ttgacggtga tgtgcgtcca cggaatgccc agaccgatcg
     6421 catgcagcgc gttccgcacc cgctcgcggc tttcggcgac ggccttgtcc ggcagcccga
     6481 cgatggcgaa cacgtgctgc ccggggctga tcctgacctc cacctgcact gggcgtgcct
     6541 cgatgcccac gaatgccaac gtggagatcc gaccgtacat gatgcccccc gcaactcctc
     6601 ttgggactga ggttaatcac cggggaatgc ttgcgtcaag aacattatga gaacttaacc
     6661 cgcaggcatg ggacggaacg aggcggcgat accatacgtt cggtacatgg agatgcagag
     6721 aatactgaat gaacgggaat gcactcatgg ccaggattgc gggtgcgagc atcgtcgtca
     6781 cgggggcctc aagtggcgtc ggccgcgcga cggcgttggc gctggcggat cacggggcgc
     6841 gcctgacgct cgctgcgcgc ggccgcgagg cactcgaacg cgtggcctcc gagtgtacag
     6901 agcgaggcgg ccctgccgtc gcggttccga cggacatttc cgattccgat gccacagtga
     6961 aactggctga ggcggcggcg agtacgttcg gaggcctgga cgcctggatc aatgtcgtgg
     7021 gagtcggtgc cttcggccgc ttcgaggaaa cgccgataga ggcgcaccgg cgggtgatcg
     7081 agacgaacct catcggcgcg gtcaacggcg ctcatgccgc gattccctat ttccgcgcgc
     7141 aggggcaggg aatcctgatc aacgtgaact cgctcggcgc gtgggtgccg tcgccttact
     7201 ccgcggctta tgccgccggg aaatggggcc tgctcggttt ctcgaaaagc ctgcgggcag
     7261 agctgtcccg gtcccggggc atcgctgtct gcgacgtctt tccgtcattc ctgaacaccc
     7321 cggggctcga tcacgctgcg aattatacgg ggcgtgaact cgccgccgcg ccccccgtct
     7381 atgagccgga ggcggtggcc aatgcgatcg tgaacctcat ccagcggcct cgatcggagg
     7441 tgacggtcgg ggttccggca aaactcgcat ggcttttggg caccatggct ccggggcttg
     7501 ccggctggtt ggctggtcgt gcgattgctg tgtacctgaa ccgcgccggc accgttccga
     7561 tcacagacgg caacctcttc gaatcaggtg gtggtacgaa ccgagttcat gggcgccaaa
     7621 cggcacagaa cagaaagttg ctgggtgcag gcctgggtac cgtggcactg gccgccgcgc
     7681 tcggtatggc gccaagactg ctccgtggtg cgccttctgc cacccctggg cgcgccggct
     7741 cgtaaggtgc aagctaggcg cgccgcgtcc gggcctccac ggcgtcccag aacagggccg
     7801 cgatgtcggg accaccgaaa tctttgatct gacgaatgcc cgtcggcgag gtgacgttga
     7861 tctcggtgag atagggaccg atcacgtcga tgcctgtgaa aatcaatccg cgccgtttca
     7921 gctccggctt gagccgttcg caaatttcga gatcccgctc cgtaagctcg gtcggcatgg
     7981 cccgaccccc cacgtgcatg ttcgagcgcg tctcgtccgg ggccgggacg cgattgatcg
     8041 cgccgaccgc ctcgccatcg atcaggatga tgcgcttatc gcctttgcga atttcaggcc
     8101 ggtactgctg gaccatgaag ggctcccgga acaccgtctg gaacagctcg accagcgagt
     8161 tcaggttcgt gtcgccttcc ggaatgcgga acaccgaaga gccgccgttg ccgtagagcg
     8221 gtttgaggat gatgtccttg tggcgctcgc ggaagcgctt cacgtcatcg agattccgcg
     8281 tcaccagcgt cggcggcatg aggtcttgga aatcgaggac ccacactttc tcgggcgcat
     8341 tcctcaccca agtcggatcg ttgacgacca gcgtcttcgg atgaatgcgc tccaataaat
     8401 gcgtcgtggt gatgtaggcc atgtcgaatg gcggatcctg gcgcaggagc acgacgtccc
     8461 attcggcaag gtccacgagc cggcggtcgg aaagcttgta atggtccccg acccgatcct
     8521 cgaccctcaa cgctgcgccc cgggcgatca gtcgcgcgcc atcgagcgcc aggttgggcg
     8581 gctcgtaata gaagagctca tgcccacgcc gctgggcctc gagcaggagc gcgaacgtgg
     8641 aatcgccgcg aatgtcgatg cggtcgatgg ggtccatctg gcaggcgatt ttcattggca
     8701 tcacatgagt ccggtgtgag cgggtcggcc cttcgatttt gaggcgctca ggtgagctgc
     8761 agggcgttcg gctgatggcg gggcaaatgc ccaggcacga tcagcatggc gtccaggccg
     8821 atctcataat cacgataggc cggatgacgc cagacccact gctctgcggc gcgggcaatg
     8881 cgccgggcct gccggatggt ctttgccgcc tcggctgctt ccatcgtcgg tcgccttttg
     8941 acttccacga aggcaagcct gcgcccgcgc acagcgatga ggtcgatctc accgagcttc
     9001 gagcgataac gccgcgcgag aatgcgatat cccttgaggc acaggaggag ggctgcgacg
     9061 aactcagcga gtcgaccgct ctgctcccgc tgtctcctga tgttgatcga ggggcgccct
     9121 tccttgtggc cgatgaaact cacgtgcgac ctcgcgtcat tcggacggcc agatcgtaca
     9181 cacgggactt gggcacgcca agcgcctctg ccacctcacg gctcgcatcc cggacgctgc
     9241 tctcggcaag gctctgttca agccgagccg cgatggcctc gtcggagacc tcggttggag
     9301 ctggcggccc gacgagaagc acgaactcgc cgcgcatgtc ctcgcctgat gacttcttgg
     9361 cgaggtcacc gagccgcccg cgcacgacct cctcgtgaat cttcgtcagc tcacgggcaa
     9421 tggcggcctc ccgctcgctt ccgagcatcg cagccatgtc ggccagtgcg gcttcgaccc
     9481 gagacggcgc ttcgaagaag atgagggtcg ccttgatgct ggcaagctcc gccaatcggg
     9541 cgcggcgcgc ggcctgcttc gttggcagga aacctgcgaa gagaaagcgg tcggtcggca
     9601 gcccgcagga cgtgagcgca gcgagcgggg ccgcgggtcc tgggatgctc acgacgcgat
     9661 aacccgccgc aactgcgctc cgcaccagct tgaagccggg gtcggacaag agcggcgtgc
     9721 cggcatcgct gatgagggcg accgctcggc ctgccgcgat ctcctccagt acacgcggaa
     9781 gctcccgatc ctcgttgtgc tcgtggaacg atctcaggtc gcggtcgatc ccgtactgca
     9841 ccaggagctg gcggctgtgg cgggtatcct cgcagtagac ggcatcggcc cgggcgatcg
     9901 tgctgatggc ccgcaacgtg aagtctgcca ggttgccgat gggcgtggcg acgagataca
     9961 gtcccggccc gagcggttgc gccagcgcat catcgagccg cgccaggacg tgccgccgca
    10021 gggattctac gctgacgtcc ccggattcct ccttgcttat ccgccgattt ttcgccaagc
    10081 cgttaccctc aaggtgcagt ttccgccgac gactagttgc aggcttcgct gagtgtcgtg
    10141 gccgtggcag cctagcctaa tccacgcctc tattgcagat ttcgatgcaa ctcttcgagc
    10201 gagcttccgg caggcccgct tcgaagtggg cgggcgaccg ggggtgacga gcgaaaatgg
    10261 catttcatgt ggcttgggtc cggcttggat cggccctcgc tctggcggtg ctcgcggcaa
    10321 ttctggcagg ctgtgccatt caaggtggcg gcgccagctt aagtccttcc ggtgacggag
    10381 ccgttgatcc gcaggactcc gcagcgcgcc cttcagtcaa ggtcgcgttg ttgttgccgc
    10441 tgacgtcgac ggggcatacc gaggctgtcg cgaaggccat gaagcaggcg gccgagctcg
    10501 ccatcttcga gctcgacaac cccaacgtgc agctcatcgc gaaggacgac aagggcacgc
    10561 cggaaggcgc gcaggccgcc gccgaagagg cgatccggga gggcgccgga ataatcctcg
    10621 ggcctctgtt tgcaggatcc gtccgcgcgg tcgcccccgt cgcagggcag gcggggattc
    10681 cggtccttgc tttctcgaac gacagccaag tggccggcaa cggcgtctat ctgcttggtt
    10741 tcccgccgca gcaggaggtt gaacgggtta tcggatacgc cctctcacga ggccatcgtc
    10801 ggttcgccgc gctcctgcca gaggacgcct acggcaagat aacggaatcg gcttttcgac
    10861 aggcggtcgg cgcgggcggc ggtgcgatcg ccgcgctcga aacctacccg ccggcgtcga
    10921 atgcgatggt cgagcccgct gaacggatcg tcgccgcgct gcgccaagcc gaggaggccc
    10981 aacagccgat cacggcgctg ttcatcccag gcggccccga ggtgctacca cggctcggcc
    11041 cgctgctgtc atatgccaac atcgacaccg cccaggtgaa gctgctcgga accggcggct
    11101 gggattttcc gaacatcggg cgcgagaggg tgttcgtcgg tggctggtac gcctcggcgg
    11161 acccgcgggg ctggcgcgat ttttcagagc ggtttgcgaa cacctacggc agctcgccgc
    11221 cgcggctcgc cagcatggcg tacgatgccg tcagcatcgt cgtccggctc tcaagcgagc
    11281 ccgcgggaac gcgattttca gcagccagtc tcacccggcc gcagggattc accggaattg
    11341 acggtccggt gcgcctgctg ccgagcggta ttgccgagcg agggcttgcc gttctcgagg
    11401 tccaggacat cggcgccacc gtcatcgatc caccgagggg cttcagcagc gcgggcagct
    11461 cctctctttc cagcggcttc aactgacaag cctttgcagg ttcgtgcagc ttgctcgcgc
    11521 gaagatgcag tttgccgaat ggcccggctc tgctaatcca cccgagaccg ctcctctttt
    11581 tccttcccgg agcgccaatc gcgctgcagt gggttcatgc tcgccatcct gaccatcgtc
    11641 atcccagtct tcggcgtcat gttcgctggc tgggtcaccg aacgctggcg cttcctgccg
    11701 gaaggcagcg ccaagacgat cgccctgttc agcttcaatg tggcgatgcc ggccttcctg
    11761 ttccgggcca tgcaggaaat cggcgaggtg ccggaagcgc cgtggaagtt cgtggtggcc
    11821 tacttcggcg gcatcgtgat cgtctgggtg gcagcggcac tggtcgctcg cacggtgctt
    11881 catcgcagtg ccgccgacgg ggccgccatc tccatggggt cctgcttcgg caacacggtg
    11941 atgctcggca taccgctggc cctcaccgct ttcggaccgg acgccgcagc gccgatcggc
    12001 attctcgtgt ccctcgattc gctggtgctg tgggtcatcg ccacactgca catcgagtgg
    12061 gcgaaccgcc aggcgcggac gacgccgttc ttccgagcgg tgggcggtgt cgttctggat
    12121 cttgtgcgca accccgtggt gatgggaatc gttgtcggat cggtctggcg cattgcgggg
    12181 ctaggtgttc ccgagctggc tgaccggttt ctcgcgctga tcggacaggc cgcggtgcca
    12241 tccggtctat tcgccctcgg catgtcgctg gcgacttaca ggatcgctgg ccagatgccg
    12301 acgctcgcga ccatcttcgc gatgaagatg ctgctccttc cggtggtggt gtattttctg
    12361 gccggatggg tgttcaacct gacgcccgtc tgggtggccg tagcgaccct gttcgcggcc
    12421 atgcctgtcg gcgccaatgc cttcctgttt gcgataaagt acgatcgtgc ggtgggttcc
    12481 gtgtcggctg ccattgccgt cagcactgtt gttgcggtcg ggaccgcatc tgccgtgctc
    12541 tacatgatgg ggatcggctg atcaccagct cgcgggggag gtggtcctcc gcagcatcaa
    12601 ccggtcccgt ggtttggtaa acaatacgga cgtgttgcaa gggtttcggc gctccgaact
    12661 gcgccgaatg ggacgagctc aagacttcct ggcttcggca accgcttctt tgagcgcttt
    12721 ctcgttcatg taaccggggt atattttccg gccgataaca aaagccggcg ttccttgaag
    12781 gttgaaagca ttcgcttggg ccatgttgcg cagaatgatg ccgtcaatat gacttctgtt
    12841 cttcttgtat ttggaataga ggtcggtcgg atcgaggccc gcttttttga gaaccgcatc
    12901 gacatcttcc cggcttaacc tccccggcgt gttcatgagt gcgtcgtgcg ccgtcgcgta
    12961 cttgccgtcg gctgccgatg cgaggacgag attggcagcg taaaccgagg gctttccgaa
    13021 gatgggaaaa tccttcatga cgagcctgat tttacgatcg ctcgcgacga ccttcttgag
    13081 ctcgggatga tttttcttac aatacgggca ttggtagtcg aagaactctg cgattgtgac
    13141 gtcgccattg ggattgccca ggactggaat gtcgggatcg aaatagacat cctcctcgct
    13201 cgggaaacct tgggcacgca aggaattcga gaaccgaggc gctgctccca aagcggttgc
    13261 agcgaaaagt gacgcagcga agaactgcct tcgatcaagc atttcagctt tctccttttt
    13321 gttttgctcc aagcacgacc acgcgcgcgc ctttagggac acgtttgtaa agatcgatga
    13381 tgtcttggtt cataaggcga atacaaccgg acgagaccgc tttgcctatg gaccagggtt
    13441 cgttcgttcc gtggatgcga tagagtgtgt ccttaccgtc cttgtacaag tacaaagcgc
    13501 gagccccgag tggattgtgg agccctcctt cgagcccatt ggcataaggg ccatagcgtt
    13561 ccggctcccg cttgatcata tcggccgtcg gagcccagcc gggccacgcc gcttttcgct
    13621 cgatgattgc ttccccctca aactccagcc cggccttggc gacgcccact ccgtagcgca
    13681 tggcttttcc gccttccata acgagataca aaaatcggtt ctgcggatcg acgataatgg
    13741 tccccaccgg ctcgtccgtt tgatagtcga cgatttggcg caagtatcga ggattggtct
    13801 ttgaagtatc gacagccgga atgatcgtcc cgttgtcgtt gacctcgccg tagggattgg
    13861 aatgcacgct gcccgggcct tcttggctcg cttccgcgac gccgactagc gaccgagttg
    13921 ttgccgtgca tccagacgcg gcgatcgcgg cagacgcgag tagagctacg cgcagggtgg
    13981 ttttaaaccc gctactcaat atatacaaac tggcctcctt atcggtcgat gttctgattt
    14041 tcgagtttcg attgcagtcc ttgcggaggg gtttgccgag agtagaagca ccggacgaca
    14101 gcagaaatgc cagtagccgg ccctggcgac ggcaatgaac cttccgcatt atgcgaggtt
    14161 ctcgggtaac gctggacaca tcagaaactc tggacctttg cggccgacct ggataggagt
    14221 tcggccgtca cctcaccaac gagccgggtt cccgcaactt ccctggcgtc gcgatcgaaa
    14281 aacaccatcg tcggggggcc gacgacgcgg agccgctgca tgagctccgg gctgtccttg
    14341 tgcagggctg aaatatcgac ctttacgagg tgaaggcctt ctaagcttcg tctgacttcg
    14401 tcgtgcggga gaacggagcg ctcgatcacg cgacaggtga tacaccagtc cgctgtgaaa
    14461 taaacgagcg ttggctttcg accgcttacg gagcgaagct ccttttgcag ctcgggaact
    14521 gatgtgacgg ttgcaaaatg caggtcgcca tctgtgctag gcggagctga gttggtcagt
    14581 ctggccagat gctgccatgg acctgtcggc gctgtcgcgg ctccgatcat cagaatcatg
    14641 ccgtaggtga tcgccatgag cccggtggtt cgggcaagca ggatgaagcc ccgatttcca
    14701 gtcgcaaagc gaaaggccca cattcctgtc ccgacgagaa gggcggccca taggacgaga
    14761 tctaagcccg cgggcaagag cggcgtcgcg atccagatag cggttgcgag aaagccgatg
    14821 ccgaaggaag tttttacatg tttcatccag gcgccagctc gcggcaggat gcttgcgccc
    14881 atcgttccga caacgattag cggtatgcct ttgcctattc ccagcgcaaa gagggcggca
    14941 gctccgagaa gcatatcgcc tgactgtgtg aggtagagaa gcgcagcagc gagtggcgcg
    15001 gtcacgcaag ggccgatgat gaagaccgag gagaacccca gaacggccac cgacgccttc
    15061 gatactttga gtttccccgt tctcgccgcg atccagttga cccagccggc aggcaaccgc
    15121 ggctcgaaaa aaccgaaact cgccagcgcc aggagaatga aggcgatcga aatggcgcct
    15181 gtcgtgaacg gcgactgcaa aacaatctgc aggttctggc cggaccaccc cgccaatgct
    15241 ccgagcagtg agaaggccga cgcagaggaa aggacgtaga ttgcggacaa gacgaagccc
    15301 cgccgcgcgg tcagccggtc tccttcacgt gccagcgtgg cggcaaggat tggatagatc
    15361 gggaagacgc aaggggtaaa cgcgatgagc aggccgaaac ccaaaaagct gaagacgacg
    15421 agcggcaggc ctcctttgcg gagcagcgat tgaacgagcc cctcttcctg agcaagacgg
    15481 aatgccgcag cgggtttctc cgcagcccca acggaggcag cgatgccagg acttggaacg
    15541 ttgccggagt cgctgttggt tgcgacctcg agtgttaggg gattgacacg cttcgtctgc
    15601 ggtacatagc atatgccgcc ttcctggcag ccctgatagg taatctcgat cggttcctcg
    15661 gaagccgcga caacggtcgc acttgcccgc ctgtagtaga cctccgtcgc tccaaagacg
    15721 ggatcgtctt tgatgctccc ctgtggagtt tcaactttga gatcagatcc gtcgacgcct
    15781 tttgcagcga tatgcgcacg gtacaggtaa tagccgtcgg ctattgtcca atcgacgcgc
    15841 acctttccgt cagtcgtcct cgaagctcgg atctggaagg cttggtcggc ggggagcgga
    15901 tcggctgcag caggtgcggc aaagatgcca aggagcacca aaccgagaat gacaaatctc
    15961 atccttctca cccccgatac tcgataggcg ttctgcatga atgctcggat cgaaggcgga
    16021 gatgtcgccg gcagcttaag gcagccttaa gccgcggatg ccacccgtga ggccccgtac
    16081 ggaatgacac accatgcgga tcttaattgt agaagacgat cctcttttgc tcgacggatt
    16141 ggctatcggg cttgggctca agggttttac ggtcgatgcc gtcgcgaact gcgaggatgg
    16201 tcgcattgct gtccgcatgc atagctacga ttgcatcgtg ctcgacgtca tgcttcctga
    16261 cggctcgggg ctggacctct tgaaagagct ccgtgcggcc aaaaattgca cgccagtgct
    16321 cctcctcacg gcgcgcgatc aggtgccaga gcggattgcg ggtctcgacg ccggcggaga
    16381 cgattatgtt ggcaagccat tcgacctcga cgagctggcc gcacggctgc gagccctagc
    16441 tcgccgtggc gccgggcgcg ccacggcgta tctggaatgg cgcggcgtgg tgctcgaccc
    16501 tgctgctctc agggcaacga aggatggagt atccctgaag ctcacgcgtc gcgaatttgc
    16561 cgtgcttcgt gccctgatgg aacgaccgac ggccatcgtc tcgaaggcgg ctttggagga
    16621 ggctctatac ggctggcagg aagagatcga aagcaacgcg gtcgaggttc atgtccatca
    16681 tctgcgggcc aagctgggtc aggatttcat caaaacggtt cggggattag gctacagact
    16741 tgcggaggag ggtgcgtgaa ttccattcgt ggccggttgc tcgcaattct catcggcacg
    16801 acgctgttcg tatggattgc cgcgatcact tggatctaca cgagtacaca ggccgaggtg
    16861 gagcgcgtgc tcgatgcgcg tttgacggaa gcggcgcgca tggtgaactc gctgttggca
    16921 gatcatagga ttccgatccg agatgacgcc acgtcgagca agccggcaat tcgcgccgct
    16981 acaaaagtct atgagcgtca actctcgtgc cagatttggt ctcttgacgg aacgctcgtc
    17041 gcgcgatccg aaagcgctcc ggggctgccg ctgacgaagg cgatggaggg tttttcggag
    17101 gatatcgtcg atggcgagac ctggcgtgtt ttcacgattg aaaaccctga gcttggcgtt
    17161 cgtgtcatgg tcggtgatac actacggatg cgtgaccggc tcgttggcga tgtcgtgaaa
    17221 ggaatggtgc attcggcggc cgtggccata ccttttctcg tcggcctgat ttggctgagt
    17281 gtgagtcatg gactcatccc tttgagcgac ctggcttgtg cgctttcgcg acgatccgcg
    17341 aacgaattat cgcctttgcc gagtgctgga attcccaagg aaattgctcc ggctgttcag
    17401 gccatcaatg agctttttgg acgggtggag gcagcccgtg aacgggaaag gagttttacg
    17461 gcatttgctg cgcacgaact gaaaacgccc ctcgctggac taaaaaccca ggcccaagtt
    17521 gcgctatcga gcaccgacgc cgctgttcac gccaatgccc tccagcagat cgtggccggc
    17581 gtggaccgga caagccgact ggttaaccag ctgctggata tggcagcact cgaggcaagc
    17641 gatgtgcaac catgctcatc ggatgaaagt ttgaaccatc ttgtgaattc cgtagttgca
    17701 gacttgcttt ccctggcatc cacgcgagga attgcgatag aggtcgatag ctcgaaagac
    17761 gacgtgcgag ccaaagttga accgcagttt ttcattcttg ccctccgcaa tttgctggag
    17821 aacgcactgg accacgcgaa aagcgtggtg agatgccagg cgctcgatca gggccagtgg
    17881 gtccgagttg tcgtcgaaga cgacggccct ggtatcctcg agccggaatt agaacgagtg
    17941 acggcaaaat tcgtgcgtgg tcgaaacaaa tcgatgacgg gaagtgggct gggcttggcg
    18001 attgcaaaaa tcgccatggc tcgcattgac ggcaagctca cgcttcataa tcgggaacca
    18061 accggccttg tcgcaatgct tgaaattcct cgccaagccg cctgatcagg caggaaccga
    18121 atccatcggc tacaccggcc gagagtattg ctggagaact gtctactcga ggtggaggag
    18181 cgcgaagacc gcgtgggcga gcggctgcga tggacctgtt caagcctggc gaccccggca
    18241 ggattcgaac ctgcgaccct cagattagga atctgatgct ctatcctgct gagctacggg
    18301 gccgatctga cgtgccgcgg aaggctgccc ttcgtactgg tttgagcaag accaccaggg
    18361 caatactagc gcggcgacga tgcgaacaaa acctccgagt gaaggtgtag ggcaggtggc
    18421 gtctgcccgc aagtgagcag aggggagcgg tggcgaaggg ggcggttcta gggatccgca
    18481 gcgcaatccg cgcgctcatt ctcgccgggc tgctggtcgg gccagtggcg gctgccgaac
    18541 ggccgggatg caagctggaa agcgggccgg cgcggggcgt tgcgaaggtc atcgacgggg
    18601 agacgctgga gctcgacgac aaatctcagg ttcggctgct tggcgcgctc gcgccacagg
    18661 cactcgacgg gggtgccgac gaaggcgcat ggccacccgc ggacgccgcg aaaactgagc
    18721 tggagaagct ggccctcggc aagtcggtcg agctcaggtt cgcaagccgg cgctctgatc
    18781 ggtatggccg actgctggcc catgtgttcg ttcgggaggg aagcgagcac acgtggctgc
    18841 aagaagaaat gctgcggcgt ggatatgcca gagcctacgc tctccccgga agcaccgaat
    18901 gcctgcagga ccttgtggcg gcggaggacc acgcgcgccg gtcgggtctc gggatttggg
    18961 gacatgcctc ctatcagatc cgtccggcag atcggcattg ggagttgctc cgctaccgga
    19021 gtacgtatca gctcgtcgaa ggcaaagtcc gggacgcggc ggaggtgcga ggtcggatct
    19081 acctgaactt cggcgagaat tggcgcgagg atttcaccat aaccgtccag ccgacgaaca
    19141 agcgtgtgtt cgacgaggct ggcatgaaac tcaccgattt gaaggggcgc cgtgtgcgcg
    19201 tacgcggctg gatcgagcgg aggggcgggc cgttgatcga gatttatcac ccaagccagc
    19261 tcgaagttct gccggagtaa cagactcgat cttcgaccgt ctgactgaag ctaaggtgga
    19321 aacgtagcag cgggccccct taacctctcc catggggagg ggtcggcccg aagggccggg
    19381 tgagggattg gaacagcagc gatcgctggc ccactccacc caaagggaag accttattcc
    19441 tcctcgccct ctgctggcat gtcgatggtc atgcgtctgg acgacaggtc gacctccggc
    19501 acgaagtctt tcgtgaaggg gaccatttcg gtattgcgac cgtctcctag ccggatttcg
    19561 agtatatcgc cggcgccgaa attgtggacg gcgaccacct cgcccagccg cgtgccatcc
    19621 ggcgtgaagg cgggaagccc aatgagatcg gagaggtaga actcatcctc ttgggtcgga
    19681 ggaaggcgct cccggctgac gtaaagttcg gtgccgcgca gcgcttctgc ggcattgcgg
    19741 tcctccacgc cagcgatccg tgccacgacg cctttgggcg tgacgcgcac gatctcgatc
    19801 tcgaactgcc gcctgccgga ctcgtcctcc aaggcgccgt aggaagcaat gtcctccggc
    19861 gcttccgtat aggtctggac gagcacgtgg ccgcgaatgc cgtgtgcggg gccgatgcgc
    19921 ccaaggagga tgcggcctga tttgacaccc atccagcaag ctccccggcg cggcggccca
    19981 gcggctgttc ggctcaggcc tggccggctt cagcggcttc cttggccttc tcggcctcgg
    20041 cggcctcgcg ctccagtcgc ttcttgccgg gcttgccttt gatcggattg tgtttcggcg
    20101 cccgctttat gagaccggcg gcatcgagga accgcgcaac gcgatccgtc ggctgggcgc
    20161 cgaccgacag ccagtgcttg cagcgctcga tgtcgagctt gacccggttc gggtcgtcgc
    20221 gcttcagcat tggattatat ttgccgacct cctcgatgta gcggccatcg cgcggcgccg
    20281 tgctgttggc ggccactatg tagtagtagg ggcgcttctt cgcgccacca cgggccaggc
    20341 ggagcttcac tgccattctg cgtcgtcctt cttctgcgtt gcttgtgttg agcttctgag
    20401 cgggaccacc tctacgggtg gcctgctatg tccaaagtgg ctgacttcat gagtcctcca
    20461 ctcgacggag agagcggcgc atcgggccgg gcaagatgcg cggataaggt ggagccacgg
    20521 gcgaaacatc ggatccgagg gcaccaccac gttcaacccc tccacgcaag gggaggggag
    20581 tttgggttgg caccggctgt gtgcgtcgga gcgccgctgc gcaccagatc gttggtcgct
    20641 aaaccccggt cgccggtcaa aacattccgt catcccgggc cttgtgcccg ggatccagcc
    20701 aacaacgaac gctggcgcca atggtgaggt ggatcccggt gacaagcacc gggatgacac
    20761 ttggggagca ggtgtcgcct gttgggaaat catctgccag cgaccccgaa ctacttcttc
    20821 ttcgccggaa aaccgggaag tcctggaaac ttcggggcgg agccgccgcc gagacctggc
    20881 aggccgccac cgaggccggg gagcctgggt acgccgccag gaggcatggg gggcagcccc
    20941 ttcggcatct cgccccgttg gagctgctcg accatctcgg gcgtcggctg gggcatgccg
    21001 ccaccgccga acatgcgggc caacatgccc ttgttcttgc ccatcgcctt catcatgtcg
    21061 gccatctgcc ggtgcatctt gagcaggcgg ttgacctcct cgaccttggt gcccgagccg
    21121 gcggcgatgc gccgcttgcg cttgccgtcg atgatctttg ggttgcggcg ctctttcggc
    21181 gtcatcgagg tgatgatggc gtgctggcgc ttcagcagct tgtcgtcgag gttcgcttcg
    21241 gcgatctggt tgcggatctt gccgatgccg ggaagcatgc cgagcacgcc ggacatgccg
    21301 ccgatcttct gcatctgctt gatctgctcg gcgaggtcct ccaggtcgaa gtcgcccttc
    21361 ttcatcttct gggcgatctt ctgcgccttc tcggcgtcga tcgtctgcgc agccttctcg
    21421 acgaggctga cgatgtcgcc catgccgaga atgcggctgg cgatgcgctc agggtggaag
    21481 tcctccagcg cgtcccactt ctcgccggtg ccgagcagct tgatcggctt gccggtgacg
    21541 gcgcgcatgg acagggccgc gccgccgcgg ccgtcgccgt cgatacgggt cagcacgatg
    21601 ccggtgagcc cgagccggcc ctcgaaggct ttggccgtgt tcacggcatc ctggccggtg
    21661 agcgcgtcgg cgacgagcag tacctcgtgc gggttcgtcg cggcctcgac ctcggccact
    21721 tccagcatca gggcttcgtc gactgtcacc cggccggccg tgtcgaggat caccacgtcg
    21781 tagccgccga gccgggcggc atccagggcg cggtgggcga tctgcaacgg cgtctggccg
    21841 gcgatgatcg gcagcgtcgg cacctcgatc tgctcgccga ggacgcgcag ctgctcctgc
    21901 gcggccgggc ggcgcgtgtc gagcgaggcc atcaacaccc gcttcttgtc gcgattcttg
    21961 agccggtaag caatcttggc gctggtggtg gtcttaccgg acccctgcag gccaaccatc
    22021 aggatcggca ccggcggtgt cgcagcgacg tcgatcggct gggcatccga gcccagcatc
    22081 cgcacgagct cgtcgtggac gatcttgacg accatctggc ccggggtgac ggacttcacc
    22141 acgtcctggc cgacggcctt ggccttcact ttctccgtga aatcgcgaac gacctccagc
    22201 gccacgtccg cctcgaggag cgcccggcgc acctcgcgca tggcctcgct gacgtcgctg
    22261 tcggtcagcg cgccgcgctt ggtcagcttg tcgaaaatgc cggagaggcg gtcggaaagg
    22321 ctctggaaca ttgctacccg tttcgctggc ggggcgctct cttaagcacc cgggaccttc
    22381 atggcctggt aatcgatcat gacgtggtcc tggtaagcct tgcgctcggt cagccgctga
    22441 taccaggcct ccacgttgcc gagccgcggg cggggtatat cgagtgtgaa atagcggtac
    22501 atgagcgaac cgcacccgat gtccgctgcc gtcggctggt ctcccagaat ataggaacgg
    22561 ccttccagct gtgcatcgag aatggcgagg cgctcgcccg cgcgctttgc cgccgcttcg
    22621 atggcccccg cattgcggtc ggcagcaggg gtgcggatca ggccgaggaa gcaggtcaga
    22681 atgatgtcgg cataaagcat cgagttggcc cactccatcc actgatcggc cagcgcgcgc
    22741 tggcgctcgt cggtggcagc gagcttgcct tggccatgga ccgctgtgag atagcgcacg
    22801 atggcgttgc tctcccagag gacgaatcca tcatcctcga tcactgggac cagccggttc
    22861 gggttgagct tgccgaattc cggcgtgtcg aggccgccaa acggtccgcc ggcatcgatc
    22921 cgctgatgcg gcaggccgag ctcgcccacc gcccacatga ccttctggac gttgatcgac
    22981 gtctttcgac cccagacctt cagcatctgt tcgcgaccct tttcagaccg gctctggcca
    23041 agcggctttc gatcactcca aacgcaaacg cgcccgcggg cgaaactcgc tggcgggcgt
    23101 tggccaccct ggcggctcta gcgccccaac cagggtagcg gctcgagaac ggagccgatc
    23161 ggaatttgag ccggaccata tggcgaacaa tcgaggctgt caaacgcaac cgaaacaggg
    23221 gcggccgatc gattgagatg ttgaggattg agatgttaag gctcgtgttc atgggctgaa
    23281 cgcgacctca atcctgattt tgcggcatga cgcgcaggat gcgccccctc gcactatccg
    23341 tggcgacgta gagcgctcca tccggtccct gccgcacatc ccgaatacgc tcgccaaggt
    23401 cggtcagcag cttctcctcg gccgtcacct gctctccgtc cagaaccagc cgcgataaat
    23461 ggcggccagc gagcgcgccg acgaacaggt tgcccttcca ttgcggagcg cggtcggcgg
    23521 tgtagaaggc catgcccgag ggggcgatcg agggatccca gtaatagagc ggctgctcca
    23581 tgccctcgcg cctctccggg atgtcctggc ccgagtagtg ccggccgtag ttgatgaccg
    23641 gccagccgta gttccgtccc ttgcggacga cgttgacctc gtccccgcca cgcggaccgt
    23701 ggtcggcgat ccagagctcg gccgtctcgg ggtgcagtgc ggcgccctgg ggattgcggt
    23761 gcccgtagga ccagatctcg ggccgagcgt tctcgcgatc gacgaacgga ttgtcgtccg
    23821 gtgcggagcc atcaggattg atccgcacga ccttgcccca gtcccgggtc aaatcctgcg
    23881 catatttcat ttgaaaacgc tcgccgaggg ttacgaacag cttgccgtcc ggcgcgatca
    23941 cgatgcgaga gccgaaatgc acgttgctgt tgaagggcgg cagctgccgg aagatgacgc
    24001 gcagctcctc aagtcgggcc tttccgtcgc tctcggcgag acgagcgcgc gccacggacg
    24061 tgccgttccc gccgccctca cgaggctcgg agaacgagaa gtagatgatc cggctgtctt
    24121 cgaaatcggg cgccagcgcg acgtcgagca gtccgccttg cccttcggcg aacactctcg
    24181 gaacgtttgc gatgggctct gaaagccgcc cgtcccgatt cacgatgcgc atgcgtccgg
    24241 gcagctcggt gaccagcagg cggtgaccgg gcagaaactg gatcgcccac gggtggacca
    24301 gaccttcggc gaccgtctcg accgtaaccc tgctttcagg cgctgcggga gctttcgtct
    24361 ggacggctgc ggcgggggtc atgaaggcaa atgtcgcgag cgtggcgatc gacatgcgca
    24421 tgcgtggcct cctgcgatga gtgcgcagta acaacgtcgg gcgaccggtt tcgtcacgtc
    24481 ggctgacgtg gtggctcctt cagccagggg tgtccgtggc cgaccgcagc aatccgctgc
    24541 acgatgcgcc gtggctcgct gagcgtggtg tgctcgtagg cgatcggcac gagccggtcg
    24601 cggacggcgt caacgagctc gccggggtta aggagaaatt cgggattggc cggcctgccc
    24661 atcctctcct ggcccaatgc gaacgtctcg tagatgagca ccccgtcagg ggcaacgcag
    24721 gtgacgatgt ccggcaggat cggacgccag aggtagttgg tgacgatcac gccggcgaag
    24781 gtgcgcccgc gcagcggaaa cgggttgccg atctccagat ccgcctcaat cagctcgacg
    24841 ccgggctggc cctttaggtc cgcaacgccg gcgaggtcgc ggtcgatgcc gacgactttg
    24901 taaccgtgcg cgagccccag ccgcagatgc cggccgctgc cgcaggcaac gtcgagcacc
    24961 tggccgccgc gcgggatccc gtgaagaaag cgcaccaccc attcagaggc gggccgcatc
    25021 tcggagcttg tcgagggctt gggcatttct gttccaattg taatccgcac ccggtccgcc
    25081 ctatatggga ccatgaccag cggcgatgcc atccccttca agaagatgaa cggcctcgga
    25141 aacgagatcg tgatggtcga tttgcgagga tgcgcacggg tcttctcgga ggaagaggcg
    25201 cgtgccatcg ccgccgaccc gcgcacccat ttcgatcagc tcatggtgct ccacgatccg
    25261 cggtcgcccg gcaccgacgc atttgtacgc atctacaata ccgacggttc ggccgcgggc
    25321 gcgtgcggga atggtacccg ctgcattgcg tgggcggtgg cgcaagagac cggtcgccgt
    25381 gatttcaggt tcgagacgag cagcggcgtg ctgcagactc acgtagagag catcgacgac
    25441 atcaccgtcg atatgggcga gcctcggttc cgttgggatg agataccgct cgccgagccc
    25501 ttccatgaca cgcgcaggat cgagctgcaa atcggcccga tcgacaatcc cgtcctgcac
    25561 tcaccctcgg tggtcaacgt cggcaacccg catgcggtat tctgggtgga cgatgttgac
    25621 gcttacgacc tcgggcgttt cggaccgctg ctcgagacgc atccgatctt cccggaacgg
    25681 gcgaacatct cactggctca ggtcacgtct ccgtcagcac tcaaggtgcg gacctgggag
    25741 cgcggcgccg ggctcaccaa ggcgtgcggc tcggccgcct gcgcggctgc cgtttgtgcg
    25801 gcacgtactg ggcgcaccgg tcgaatggtc acggtgacgc tgcctggcgg gatgcttcac
    25861 attgaatgga cggcggccaa caggatcctc atgcgcgggc cggctaggct ggagtacgag
    25921 gggttgcttg accccgagct gttcgtgaag actccggcgt aggtgcctgt catctttgcc
    25981 cattgtctgg tcgcaacttg gatcaggacg tggtatctgc cgtagctggc ggaaaaaaga
    26041 actgcccggc ctccccgacg agggcgggtg cacaattcgc atctcaaagt ttcgaatgtc
    26101 cgaccctact gtcatcaccc tcggctgtcg cctgaatgcc tacgagtcgg agctcatgcg
    26161 ccggcatgca gccgaggccg ggctcgagga cgcggtcatc atcaacacgt gtgcggtgac
    26221 ggccgaggct gtgcgacagt cgcagcaggc cattcgcagg gcgcggcgcg agcggccaga
    26281 tgccaaggtg atcgtcacag gctgcgcggc acaggtcgac ccagacctgt ttgccggcat
    26341 ggccgaggtc gaccacgtca tcggcaatgc cgagaaggtg aagcgggaga cctttctggg
    26401 cctgaacctg gcgaacagcg agcgcgtgca ggtcaacgac atcatgagcg tgcgtgagac
    26461 ggcgtcgcac ctgatcgacg gattcggcac gcgcgcccgc gcctacgtgc aggtccagaa
    26521 cggctgcgac caccgctgca ccttctgcat catcccttac gggcgcggcc catcccgatc
    26581 cgttccggct ggcgaggttg tcgcgcaggt gcggcgtctc gtcgaggcgg gctacctgga
    26641 ggtggttctg accggcgtcg acattacctc atacggcaac gacttgcctg ggcggatgtc
    26701 gctgggccga ctggtccgcc agatcttgcg tcatgtgccc gagctcaagc ggctacgcct
    26761 ctcctccatc gaccaggtgg aggcagacaa agatctgatg gacgccattg ccgaagaaca
    26821 gcgattgatg ccgcatctgc acttgtcact tcaggcgggc gacgacctca ttctgaagcg
    26881 gatgaagcgc cgtcatttgc ggagcgatgc gatccggttc tgcgagacgg cgcgccggct
    26941 cagacctgat gtcgtcttcg gcgccgatct gatcgctggc tttcccacgg agaccgaaca
    27001 gcatttcgag aacacgctgc gcctcgtcga agagtgcggg ctgacctacc ttcatgtctt
    27061 tccgttttct ccgcgaaagg gcacgccggc agcacgtatg ccgcaggtgg accggaagga
    27121 agtcaaggag cgggcagccc ggctccgcag cctgggcagc aaggttcttg cccggtttct
    27181 ggagcaacag cacggcagcg tcgtcgaggt gttgacagag ggggcgaatg tcggccgcac
    27241 ggcccagttc gctgaagtca aactgtccga cgaagtcccc gcgggcgctc tgctgagcgc
    27301 cagaatccag ggagacgacg gccagcgcct tgccggagag gtgcttgcgg gatgagcatg
    27361 aagaagccga agaagggtgg gcttctcgat cgcctgttcg gccgcggcga gaagaccgcg
    27421 gaagatggcg aggcggacgt ccgcctgaaa gagcccatct cgccttcaga ggccgagggg
    27481 ccgcagactt ccgaagaaca gctggaatcc agcgaaacga cagagacggt gccgccggct
    27541 gacgccatga cggcggaggc ggaggcttca ttcgagcagg caccgcggga agaagcggga
    27601 gccgaggcgg cgcccgaaac gctcgagccg cccacgaaga cgagctggtt cagtcgcctc
    27661 aaggcagggc tgacgaagac atcgagccgc ctgtccgaag gcatcaccgg cgtgttcgcc
    27721 aagcgcaagc tcgacgcgga cacgctcgac gagctcgagg acctgctcat tcaggcggac
    27781 ctcggattgg aaacggcggc ccgcatcacc gatgcccttt cgcagggccg gtacgacaag
    27841 gatatctccc ccgacgaggt gcgtgcggtc ctcgcatccg aaatcgcgaa ggttctggag
    27901 cccgtagccc agccgcttga gatcaaagcc gatgccaagc cccatgtgat cctcgtggtg
    27961 ggcgtcaacg gcaccgggaa gaccacgacg atcggcaagc tcgcggccaa gtacacccgt
    28021 tcgggcaaga aggtcatgtt ggccgccggc gataccttcc gcgccgctgc catcgatcag
    28081 ctcaaggtgt ggggcgagcg gacgggcgcg accgtggtcg cgcgcgatgt cggcgcggat
    28141 gcggcgggtc ttgccttcga tgccttcacg caagcgcgca aggaaggctt cgatgtcctg
    28201 ctcatcgata cggcaggccg gctgcagaac aagcaggccc tgatggacga gcttggcaag
    28261 gtcacccggg tcttgaagaa aatcgacccg gcggtgcccc atgatacgct gctcgtgctg
    28321 gacgcgacca ccggccagaa tgctctggac caggtgcagg tctttcagaa gcaggccgat
    28381 gtcagcggtt tggtaatgac taagctggac ggtacggctc ggggcgggat cctcgttgcc
    28441 atcgcagcca agtaccggct tccggtccac gcgatcggtg ttggggaagg cattgacgat
    28501 ctgcaggcgt tcgacgcggg agaattcgcc cgagccatag cgcttgcata gacattatag
    28561 ttttgacgat caggggaggc atcagagcgg gacgatgagc tggttgaggc gtacctatcc
    28621 attcaacgcc gagcagactg tcaatattct gagtgagttc gggcctctgg tgaccatgtt
    28681 catcgtcaat gcgatgtacg ggatcagcgc cggtacctgg gccctgatca tcacgaccgg
    28741 gatcgcgatc gtaacgatgc gcaaagttct cggacggttt ccggcgttcc ccatcattgc
    28801 cagcagcgtg acgatcacct tcggcttcct gacgttgttc accggcgacg ccatgtggat
    28861 ccagatcaag gtcacgatct tcaatttgtt gtttgcaatt ttcctcttcg gcgggctctg
    28921 gatggggaag aatttctttc agtacgtttt cgagagcacg ttccactaca cgaagaaggg
    28981 ctgggatcgc ttcaccttca gctttgcgtg cttcttcctg ttgaccgcga ttttgaacga
    29041 gatcgtgcgc cttactttcg atgaccacac gatctatagc ttgcttggct atcaaatgga
    29101 cggggtgaac gtctggatcc tgttcaaggt cgcgttcatc atgccggtga gcggactata
    29161 cgcttggtgg ctgacccgtc tgatgcaaaa gtaccgcatt cggaccgact cgggcagcgt
    29221 tcctggcgac gcgctcgcat ccgctgccga gcggattggt cgcaagcaaa ggccggcgac
    29281 gcaaaaggct tgagcctgcg tcctgctggt gtcacccgca gtcccaccga cgtgaggttc
    29341 tcccttgtct cagcaatcgg caaccaagca agagccggcc cggagcgagc gggcccagct
    29401 cctgaagctc ggtctcgagg tggcgccgat cgttatcttc tttctcgtga acagtcagcg
    29461 cgacatcttc tggggtaccg gcgctttcat ggtggcgacg atcat
//
LOCUS       DASVDU010000039        11828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_712709, whole genome shotgun sequence.
ACCESSION   DASVDU010000039 DASVDU010000000
VERSION     DASVDU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11828
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712709"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..283
                     /locus_tag="VF226_02780"
     CDS             62..283
                     /locus_tag="VF226_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072939.1"
                     /translation="MSDSYRTVTALVKVVRPNAIAVERPRGPGNTWIPRSLIHGGDDL
                     KIDGLCFGSEGIWHTFRLVEWKAEEAGLS"
     gene            280..438
                     /locus_tag="VF226_02785"
     CDS             280..438
                     /locus_tag="VF226_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072940.1"
                     /translation="MTALTTFRVFYSDGSTVDVAAANPYDARAAARKQRDGQIKKVKR
                     VKEAGVNG"
     gene            431..1330
                     /locus_tag="VF226_02790"
     CDS             431..1330
                     /locus_tag="VF226_02790"
                     /inference="COORDINATES: protein motif:HMM:NF019146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage Gp37/Gp68 family protein"
                     /protein_id="HEX7072941.1"
                     /translation="MAENSKIEWTEATWNPIAGCSVVSPGCTNCYAMRRVAPRLSRNP
                     ATPHYAGTVTPSKAGPVWTGEIGLASDEVFTKPLRWKTPRMIFVNSTSDLFHEKVPDE
                     VIDRVFAVMAEAHWHTYQVLTKRSKRMHNYLSAEIVTERIGRIQQRDHGDNCAYTNVA
                     LDAIWPLPNVWLGVSAEDQKRADERIPDLLATPAAIRFVSAEPLLEPLSLERWLPIAA
                     RGNCYDAVKCEAAYGEKRPRIDWVIVGGESGRGARDNDFITNARSILAQCKAADVPFF
                     GKQNVEKRPLPADLMVREWPEAS"
     gene            1332..1928
                     /locus_tag="VF226_02795"
     CDS             1332..1928
                     /locus_tag="VF226_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072942.1"
                     /translation="MANGLPCIIVAGMGRCGTSLTMQMLEAAGVPCIGGWPDFETDAS
                     SIEGFDPSWFAGLSGCAIKLIDPANLPITDMPNHIVVWLDRDPTQQAKSQAKLLKVLG
                     LNIGRSATRTLAADLRNTREKHRARVGVPGGCPATTLRFENMITDPAFAAERLSAFLA
                     SYGWATDPEAMAAQVKPRPATCLPGFLEADLLESRGMA"
     gene            1930..2343
                     /locus_tag="VF226_02800"
     CDS             1930..2343
                     /locus_tag="VF226_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018445576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX7072943.1"
                     /translation="MRREFTTATRKAALKRSEKRCEAVGSWYGLPEGQRCTADLSLGV
                     EYDHYILDANSKDNSLENCRAVCPKCHSWKTQHHDIPTAAKTVRQQFMGMKTKVKTKI
                     QSRGFEKKARVPKPSLKPRLLYQEAKSRNEIHRDS"
     gene            2321..2482
                     /locus_tag="VF226_02805"
     CDS             2321..2482
                     /locus_tag="VF226_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072944.1"
                     /translation="MKYIVTVEYTTTRQKELTIYAGSEDEAEQKACDIVLGWNDVDDA
                     EAIEIEEAD"
     gene            2482..2724
                     /locus_tag="VF226_02810"
     CDS             2482..2724
                     /locus_tag="VF226_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072945.1"
                     /translation="MKKIPDEIMREADRIYGQLGRGVASDVTAIVRALLARDQRAAEI
                     ARKHRPSWLSPNTAHLRQKADSIARAILTYDEAHHD"
     gene            2717..2875
                     /locus_tag="VF226_02815"
     CDS             2717..2875
                     /locus_tag="VF226_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072946.1"
                     /translation="MTETTPDYAGLSRRLGALAYHIQQTGTADSQDAQTCLEVDDALR
                     TLEAQEGK"
     gene            2877..3695
                     /locus_tag="VF226_02820"
     CDS             2877..3695
                     /locus_tag="VF226_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072947.1"
                     /translation="MAETVTQFAARVLGIADCPPPQPKRGPQYGTGAVCWLCGGATDG
                     KGWPQATAIAPTFTQHNTAKANDSDAVCQSCAALTRAETFQRMVAERGLSVKTWTQAG
                     WHSYSHFIRDDGHYSVPSRKDMRALLLDPPPGRWLLCINTTGQKHTIFRATVASSRTL
                     FPVQMDETTIWCRADEIRQCMADFEGLCALGFSKDSILTGQYHPAQLLKAGLSRWKPA
                     EGRIAPWRQSHPDLMALVHFVALGPAEFEPIEAAEYERSAVPPQRQAQGQMEMF"
     gene            3697..4197
                     /locus_tag="VF226_02825"
     CDS             3697..4197
                     /locus_tag="VF226_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072948.1"
                     /translation="MIYGFETDDENAAIAALIVYVVWRSRDRVKFKITPDVWGQVERF
                     TKDAAKRAKTIPDFVEALKKPGRLYAPTLHPRYMEVGAKGEVPLTIIRNPDGSLREAI
                     QFGADRDIGLREFGTRVIERADAQAVIRAAYKTTSYIILLVRDRLEREKPIEQHLDSL
                     IDGDAA"
     gene            4305..5162
                     /locus_tag="VF226_02830"
     CDS             4305..5162
                     /locus_tag="VF226_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072949.1"
                     /translation="MQPNGTVEEVFCYSGNAWRGQLRDQMGHYMLESLGHVKIALDPF
                     HLIFSGGRIGGAQNTDIEQARRIRAAMPVVSVLGGGVGNQILEGKLIVSNCYPVCREA
                     IPVLPSNLHEQAETIAYADCTFEKEHSRRDDSKIESVRKHLLTEETLLLEGGKSKKAE
                     KDGPADQMRIGMELVCPGVTLTTSLVLRMASEVELGALVSGIHRFSQEPHIGGKIGIG
                     YGLVDLHYSLRADDGEHSDFITVQDGQSLLSPVASEAKDAYDQHLRSIYDAYLTDNAS
                     GIRQLLGAA"
     gene            5162..5749
                     /locus_tag="VF226_02835"
     CDS             5162..5749
                     /locus_tag="VF226_02835"
                     /inference="COORDINATES: protein motif:HMM:NF020377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HEX7072950.1"
                     /translation="MVWVAEVHRDKSMPLDQVHVALYSYFPQVAPGDPRPYVWRLLDE
                     NTILVGSRLRPTANTAREVVARRGMTYDFRATIKRARNVRGTYTRPDGTRRNRTPRAV
                     EMKDHDEIKRWATQFAEKRGGDIRYIRVENMCLYRPKHGVALPICTLVGKVFVRDPDA
                     FDQFLASGGPGTGKAYGLGMWYLPEIMGEVHRAAA"
     gene            5736..6392
                     /locus_tag="VF226_02840"
     CDS             5736..6392
                     /locus_tag="VF226_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072951.1"
                     /translation="MQPLRIRAHLRNGYAAKDPWSPAIDGILAAVVMRRKLGDEYATN
                     AAQPSKLEPVEGLPLEVCRWGDKWWYAASSPIIVGFSGREKKAYHRRFDDQHERHLVD
                     GIRRVMTAAGPYKNTRLHDTRVICRALEWHVIGDEAPIRDLLSDVEQIGGRRGIGYGW
                     VTEWQISAGDPRIARGHRPLPVEVQESQDGMVMPWGIVPPGRWRPIECWMPEERHAEA
                     "
     gene            6379..7164
                     /locus_tag="VF226_02845"
     CDS             6379..7164
                     /locus_tag="VF226_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEX7072952.1"
                     /translation="MQKHERETYRLHAKLPVHARRIERAKRIVREAMDRVPGIWGVGL
                     SGGKDSIVLAHICCEAGWDGPLFHYHAAEIPEENTMIALAMGRRLNREVITEEIEGDW
                     ALWEKAGRAFFEPETDLERKLMSGQSARYKRQIADAVAAHGLVGLFWGIRKEESRART
                     IAIAKHGVIYKAVSRHEWTAHPLADWSARDVWAYLVAHDLPWLDRYDRAEDRERERSE
                     TTFIFGRGGDGLWAMGQGQRLRESDPALWARLCQRWPDLRKYG"
     gene            7161..7541
                     /locus_tag="VF226_02850"
     CDS             7161..7541
                     /locus_tag="VF226_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072953.1"
                     /translation="MMEEQDDHANPRIGRKQMSRARRFTKAKEVRRGGGVFDYEPVLS
                     AGEMYEIALDALSALTAGKPEQVPSDEHRYAERLLIALMEKHYPDRSPEWAPLPDLMG
                     ILTQIDNLTTGLVRAAPTAGGGDA"
     gene            7764..8105
                     /locus_tag="VF226_02855"
     CDS             7764..8105
                     /locus_tag="VF226_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072954.1"
                     /translation="MSARDIIADEIAISRGRRPDECVHGNGWVSTDGSGFECSLTARQ
                     QAYPVANAVLQSLTAAGYRILAPGQYDPVTAARALDAISPAEFDSDTVVRVREAIRRA
                     LKAGIAEGGGK"
     gene            8302..9432
                     /locus_tag="VF226_02860"
     CDS             8302..9432
                     /locus_tag="VF226_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7072955.1"
                     /translation="MPQKRKPPRLWLRTETGSDGRKRSTYIILDGAKHIRTGCSEDEA
                     EEASRKLAEYTFSKYEAPRGGRASEITISDVLIVYLDEKADATARPKETKAAINRLNE
                     FFGSDTISAIKGKRCRDFVADRKTQAGARRDLEVLRAAVGYYHKEYGLDVLPAFTLPE
                     KGQPRERYLTRSEAAALLWACLGWQKETIGTGPNSYEKWKRRRYHRANHLARLVLIGL
                     YTGTRPGAIKQLQWMRNTTGGWADLDRGVIFRRAEGERVAHNKRKPPVKLARRLLAHM
                     KRWKRMDGWEDDQVGLRYVVHYYGKPLTKENKAFRSAIAAAGLSSDVTPHILRHTRGT
                     WLAQAHVPPNEAAASLGLTVEEYERTYLHNDPDFQKAAADAY"
     gene            9792..9992
                     /locus_tag="VF226_02865"
     CDS             9792..9992
                     /locus_tag="VF226_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072956.1"
                     /translation="MAHRKIVEGSTLTLEVTVIRVWDDGRVTFRLYDHDAPITIPGDA
                     PNIILVSDPENVPRKKQKPRQR"
     gene            complement(10245..10574)
                     /locus_tag="VF226_02870"
     CDS             complement(10245..10574)
                     /locus_tag="VF226_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1515 family protein"
                     /protein_id="HEX7072957.1"
                     /translation="MPTSTNIERALGVVIGKLESIEDRLHRQDESRAALHKRMDDLVV
                     RTTSLESEVTGIKGTIRDVQKVTEEVKAWKQRGIGALAIVGMGASALTWLITTYFDTI
                     MRMLGRN"
     gene            complement(10812..11063)
                     /locus_tag="VF226_02875"
     CDS             complement(10812..11063)
                     /locus_tag="VF226_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072958.1"
                     /translation="MDFILSLIPGGSLTAIGAGIVAALLAVWRIFAAGRKAGRNEAAA
                     KERDAYESHLQDIARANHARAGAELGGVPDSDPYNRDRH"
     gene            complement(11063..11275)
                     /locus_tag="VF226_02880"
     CDS             complement(11063..11275)
                     /locus_tag="VF226_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072959.1"
                     /translation="MWQKLLAWARDSETILWARLNVLFGIVLQAIAMVDPQMVVPYID
                     PKYVPLWLIFSGIVTEIARRMRADDV"
     gene            complement(11291..>11828)
                     /locus_tag="VF226_02885"
     CDS             complement(11291..>11828)
                     /locus_tag="VF226_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HEX7072960.1"
                     /translation="AQVAKVYRKHYWDKVKGDDLPSGIDYAVFDFAVNSGPTRAAKFL
                     QEVLRVAVDGKIGPQTLAAARRANAADVIQALCDKRMAFLRGLKNWPTFHKGWTSRVA
                     AVRAVALEMAPAKPAQAQKPAPSVPEPQTPETRSTEPGDAKAGALVAVLVALAATAAM
                     VWEWLASLPCDLLGILCN"
BASE COUNT         2448 a         3590 c         3642 g         2148 t
ORIGIN      
        1 agcgcgagga gatcgcccct gcccccgtcg agcgccttgt tccccggagg gcggggaagt
       61 catgagcgat tcctaccgca ccgtcacggc cttggtgaag gttgtccgcc cgaacgcaat
      121 agccgtcgaa cgccctcgcg gcccggggaa tacctggatt ccgcgctcgc tcatccatgg
      181 gggcgatgac ctaaagattg atggcctctg cttcgggtct gagggcatct ggcatacgtt
      241 ccgcctagtg gagtggaagg cagaggaggc gggcctgtca tgaccgccct caccacgttc
      301 cgcgtcttct attccgacgg ctccaccgtc gatgttgcag ccgcgaatcc ctacgacgcg
      361 agagccgctg ccaggaagca gcgcgacggg cagatcaaga aggtcaagcg cgtgaaggag
      421 gccggggtaa atggctgaga acagcaagat cgagtggaca gaggcgacgt ggaatccgat
      481 tgccggctgt tccgtcgtct cgccgggctg tacgaactgc tatgccatgc gccgcgttgc
      541 gccgcgtctt tcacggaatc cggccacgcc gcattacgcc ggcacggtca cgccgtcaaa
      601 ggcgggcccg gtctggacgg gcgagatcgg cctcgcatcc gacgaggtct tcacgaagcc
      661 gctgcgctgg aagactccgc gcatgatctt cgtcaattca acctcggatc ttttccacga
      721 aaaggttccc gacgaggtga tcgatcgcgt cttcgccgtc atggccgagg ctcattggca
      781 cacctatcag gtgctcacca agcgctcaaa gcggatgcac aactatcttt cggccgagat
      841 cgtgaccgag aggatagggc gcatccagca gcgcgatcat ggcgacaact gcgcctacac
      901 caatgtcgcc ctcgacgcga tctggcccct tcccaatgtt tggctaggcg tctcggccga
      961 ggaccagaaa cgcgccgacg agcgcattcc cgatctcctc gccacgccgg cggcgatccg
     1021 ctttgtctcg gccgagccgc tgctcgaacc attgtctctc gagcgatggc ttccgatcgc
     1081 tgctcgcggg aactgctatg atgccgtgaa atgcgaagcg gcgtacggcg agaagcgccc
     1141 ccgcattgat tgggtgatcg tcggcggcga gagcggacgc ggcgcgcgcg acaatgactt
     1201 tatcaccaac gctcgatcga ttctagcgca gtgcaaagct gccgacgtgc cgttctttgg
     1261 caagcagaac gtcgagaaga ggccacttcc cgctgacctg atggtccgcg agtggccgga
     1321 ggcgtcctga catggcgaat ggtctcccct gcataatcgt cgccgggatg ggccgttgtg
     1381 gcacctccct aacaatgcag atgcttgaag cggctggcgt cccctgcatt gggggctggc
     1441 ccgatttcga gacggatgcg agttcaatag aaggtttcga cccatcatgg ttcgctggct
     1501 tgtccggttg cgccatcaaa cttatcgatc cagccaacct gccgatcaca gacatgccaa
     1561 accatatcgt cgtttggctg gatcgtgacc cgacgcagca agcaaaatcc caagccaagc
     1621 tcctcaaagt ccttggcctg aatatcggtc gctcggcaac aagaactctc gccgctgatc
     1681 ttcgcaatac gcgcgagaag catcgggcgc gggtcggcgt acccggtgga tgtcccgcga
     1741 ctacgctccg cttcgagaac atgattaccg atccggcttt tgctgcggag cggctgtcag
     1801 ctttcctcgc atcttatggc tgggcaaccg acccggaagc aatggcggcg caagtgaagc
     1861 cccgaccggc tacttgtctg cccggattcc ttgaagcgga ccttttggaa tccagaggga
     1921 tggcctgaca tgcgccgcga gttcacaacc gcaacccgca aggctgctct caagcgctcg
     1981 gaaaagcgtt gcgaggcagt tggttcttgg tacggcttgc cagaagggca gcgctgcaca
     2041 gcggatctga gccttggcgt cgaatacgac cactacatcc tcgacgccaa ttccaaggac
     2101 aacagcttgg agaattgccg cgcggtctgc ccgaagtgtc attcctggaa gacacagcac
     2161 cacgatatcc cgacagcggc caagaccgtg cgccagcagt tcatgggcat gaagaccaag
     2221 gtcaagacga agatccaatc ccgcggtttc gagaagaagg ctcgtgttcc caagccgagc
     2281 ctaaagccgc gcttactcta tcaggaggcc aagagccgaa atgaaataca tcgtgacagt
     2341 tgaatacacc acgacgcgcc agaaggaatt gacgatctac gctggcagcg aagacgaagc
     2401 cgagcagaaa gcgtgcgata tcgtcctcgg ctggaatgac gtggacgacg ccgaggctat
     2461 cgagatcgag gaagccgact gatgaagaag ataccagacg agatcatgcg ggaggccgac
     2521 cgcatctatg gccagcttgg ccgaggtgtg gcgagtgacg ttaccgcaat tgtccgcgcg
     2581 ctccttgctc gtgaccagcg ggcggcagag atagcgcgca agcaccgccc ttcttggctg
     2641 tccccgaaca cggcgcatct gcgacaaaag gccgacagca tcgcccgcgc catcctcacc
     2701 tacgacgagg cccaccatga ctgagactac accagactat gcggggctat cacgtcgcct
     2761 cggcgcgctg gcgtaccaca ttcagcaaac cggaacggcg gacagccagg acgcgcagac
     2821 ctgccttgaa gttgatgacg ccctccgcac ccttgaagca caggagggca aatgacgtgg
     2881 ctgaaacagt gacgcaattc gctgcgcgcg tgcttggcat tgccgactgt ccgccgcctc
     2941 agcccaagcg cgggccgcaa tacgggaccg gcgccgtatg ctggctctgc ggcggggcga
     3001 ctgacggcaa aggctggcca caggcaaccg ccatcgcccc caccttcacg caacacaata
     3061 cggcgaaggc gaatgacagc gatgctgtgt gccagtcctg tgctgctctg acgcgcgctg
     3121 agacgttcca acggatggtt gctgaacgcg gcctgtcagt gaagacgtgg acccaggcag
     3181 gctggcacag ctacagccac ttcatccgcg atgacggcca ctattcggtc ccgtcgcgca
     3241 aggacatgcg ggccctcctc cttgacccgc ctcccggccg ctggctgctc tgcatcaata
     3301 cgaccggtca gaagcacacg atctttcggg cgacggtcgc ctcgtcgcgg acgctgtttc
     3361 ccgtccagat ggatgagacc acgatctggt gccgtgccga tgagatcagg cagtgcatgg
     3421 ctgatttcga gggcctgtgt gcgctcggct tctcgaagga cagcatcctc accggccagt
     3481 accatccagc acagctgctc aaagcgggac ttagccgctg gaagcccgcg gaaggtcgaa
     3541 ttgcaccttg gcgtcagtct cacccagacc tgatggctct cgtgcatttc gtcgccttgg
     3601 ggccagcgga attcgagccc atcgaagcag cggaatacga gcgtagcgcc gtgccgcctc
     3661 agaggcaggc acagggacag atggagatgt tctaggatga tctacggctt tgaaacagac
     3721 gacgaaaacg cggccatcgc cgcactgatt gtatatgtcg tgtggcgcag ccgcgaccgg
     3781 gtaaaattca agatcacccc ggacgtgtgg ggccaggtcg agcgtttcac caaggatgcc
     3841 gccaagcgag caaagacgat ccccgacttc gttgaggcat taaagaagcc gggccgtctt
     3901 tacgcaccga cgctgcatcc ccgctacatg gaggtcggcg ccaagggcga ggttccgctg
     3961 accatcatcc ggaacccgga tggctccctt cgcgaggcga tccaattcgg cgcggaccgc
     4021 gatattggac tcagggaatt tggcacacgc gtgatcgagc gcgccgatgc gcaggccgtg
     4081 atccgcgccg cctacaagac caccagctac atcatccttt tggtccggga ccgcctcgag
     4141 cgcgagaagc ctatcgagca gcacctggac agcctcattg atggagacgc cgcatgaccg
     4201 agctcaagac ctatcgcttg gagggctttt tccggctcaa gtcgcccctg tcccacatcg
     4261 gggagagcat cagcaacaag tcctacctcg tgcaggagcg aatcttgcag ccgaacggaa
     4321 cggtggaaga ggtattctgc tattccggca atgcctggcg tggccaactg cgcgaccaga
     4381 tggggcacta catgctggag tctctcgggc acgtcaaaat cgcgctggac cccttccact
     4441 tgatcttctc cggtggccgt atcggcggcg cccagaatac cgacatcgag caggcacgcc
     4501 gcatccgtgc ggcaatgccc gtggtgtcgg tcttaggcgg gggtgtgggc aatcagatcc
     4561 tcgaaggtaa actgattgtc tcgaactgtt atcccgtttg ccgcgaggcg atccctgtct
     4621 tgccgtcaaa tctgcacgag caggccgaaa cgatagccta cgcagactgc acctttgaaa
     4681 aggagcactc acgccgtgac gacagcaaga tagagagcgt ccgcaagcat ctgctgactg
     4741 aagagacttt gctgcttgaa ggaggcaagt ccaagaaggc tgaaaaagac ggaccagccg
     4801 accagatgcg catcggcatg gagcttgttt gccccggcgt gactctcact accagccttg
     4861 ttcttcgcat ggcttcagaa gtcgagcttg gcgctctcgt ctccggcatt catcgctttt
     4921 cgcaggagcc gcatatcggc ggcaagatcg gcatcggcta tggcctcgtt gatctgcatt
     4981 acagcctgcg cgccgatgac ggcgagcact cggacttcat caccgtccag gatgggcagt
     5041 cgcttctgtc gcctgtggcg agcgaggcga aggacgccta tgaccagcac ctccgctcga
     5101 tctacgacgc gtatctcact gacaatgcat ccggcatccg acagcttctg ggggcggcgt
     5161 gatggtgtgg gttgcagaag tccatcgcga caagtccatg cccctggacc aggtgcatgt
     5221 cgcgctttac agctatttcc cgcaggtcgc tcccggcgat ccgcgcccct atgtctggcg
     5281 gcttctggat gagaatacga tcctcgtagg atcgcggctc aggccgaccg cgaataccgc
     5341 ccgtgaagtc gtcgctcgac gcggcatgac ttacgatttc cgggcgacga taaagcgggc
     5401 acgcaatgta cgcggcacat atacacgacc cgatgggacc aggcgaaatc gcacgcctcg
     5461 cgcggtcgag atgaaagatc acgacgagat caagcgctgg gccacgcaat tcgccgaaaa
     5521 acgcggaggc gatatccgct acatccgcgt cgagaacatg tgcctctacc gccccaagca
     5581 tggcgtggcg ctcccgatct gcactctggt cggcaaggtc tttgtgcgtg atcctgatgc
     5641 ctttgaccaa tttctagcct ctggtggacc gggtacgggc aaggcttatg gacttggcat
     5701 gtggtacctg ccggaaatca tgggggaggt gcaccgtgca gccgcttagg atcagggcgc
     5761 atctgcgcaa cgggtacgcg gcgaaggacc catggtcgcc agcgattgat ggcatcctgg
     5821 ccgccgtcgt catgcggcgc aagctggggg acgagtacgc gacgaatgct gcccagccct
     5881 cgaaattgga gccagtcgag ggactgccgc ttgaggtgtg ccgatggggc gacaagtggt
     5941 ggtatgcagc ctccagcccc atcatcgtcg gcttctctgg gcgcgagaaa aaagcctacc
     6001 atcgccgctt tgacgatcag catgagcgtc atctggtcga tggcatccgc cgcgtcatga
     6061 cggcggctgg gccatataaa aacacccgcc tgcacgacac gcgtgtgatc tgtcgcgcgc
     6121 tggaatggca tgtcatcggg gacgaggccc ctatccgcga cctgctttcc gatgtcgagc
     6181 agatcggcgg tcgtcgcggg atcggctacg gctgggtgac cgagtggcag ataagcgcgg
     6241 gtgatcctcg catcgcgcga ggccacaggc ctctaccggt ggaggtacag gaaagccagg
     6301 atggcatggt gatgccgtgg ggcatcgtcc cgcccgggcg atggcggccc atcgaatgct
     6361 ggatgccgga ggaacgccat gcagaagcat gaacgcgaga cataccggct gcatgccaag
     6421 ctgccggtcc atgcgcggcg catcgagcgt gcaaagcgca tcgtgcggga agccatggat
     6481 cgcgttccag ggatctgggg tgtgggacta agcggcggaa aggacagcat tgttctggcc
     6541 cacatctgtt gtgaagctgg ttgggacggt ccgctgttcc actaccacgc agccgaaatc
     6601 ccggaagaga atacgatgat agcgcttgcc atgggccgaa gactcaaccg cgaggtcatc
     6661 acggaagaaa tcgagggaga ctgggcactc tgggagaaag caggaagagc ttttttcgag
     6721 ccggagacgg acctggaacg caagctcatg tccggtcagt cagcacgata caagcgacag
     6781 atagccgacg ccgttgcagc acatgggtta gtgggtctgt tctgggggat caggaaagag
     6841 gaaagtcgcg ccaggaccat cgcgatcgca aagcatggcg tcatctacaa ggccgtgagc
     6901 cgccacgaat ggactgccca cccattggcc gattggtcgg cgcgagacgt gtgggcctat
     6961 ctcgtggctc atgacctgcc atggctcgat cgctacgacc gggcggagga tcgtgagcgc
     7021 gagcgctccg agacgacgtt cattttcggt cggggcggcg atgggctttg ggccatgggg
     7081 cagggtcaac ggctccggga gagcgatcct gccctgtggg cccgcctctg ccagagatgg
     7141 cccgacctca ggaaatatgg atgatggagg agcaggatga tcatgccaat cctcgcatcg
     7201 ggagaaagca gatgagtagg gcgcggcgct tcacgaaggc caaagaagtt cggagaggtg
     7261 gcggggtgtt cgactatgag cccgtcctgt cagcaggaga aatgtacgag attgcactcg
     7321 atgcgctctc cgccctcacg gcaggcaagc cggagcaggt gccaagtgat gagcaccgct
     7381 acgccgaacg gctccttatt gccctgatgg agaagcatta cccggatcgc tcgccggagt
     7441 gggcacctct ccctgacctt atgggcatcc tgacacagat tgacaacctc acaactgggt
     7501 tagttcgcgc cgcccctaca gcaggaggtg gcgatgcgta gactgcgacc gcctgaaatc
     7561 agagcattgg ccgttctgct tgatggtcca aaaccaactt ttcgatttgc ggtgcgtgac
     7621 ctgcgatcat gggagcagtt gcaccttata ggctacatcc aacatgagga cggttcgcgc
     7681 tcggttatga tcaccgaagc aggccgcgcc gccctccgcg ccctatccga ccaggataca
     7741 ggccaaggtg aggagagcgg gcgatgagcg cacgagacat cattgcggat gagattgcaa
     7801 tctcgcgtgg ccgtcgccct gacgagtgcg tgcacggcaa cgggtgggtg agcaccgacg
     7861 gctccggctt cgaatgctca ctgacggcgc ggcaacaagc atatccagtt gccaacgccg
     7921 ttctccagtc gctcaccgcc gcaggctacc gcatcctcgc ccccggccaa tacgaccccg
     7981 tgaccgcagc tcgggcactg gacgccatct ctcccgctga attcgacagc gatacggtgg
     8041 tgagggtgag agaggcgatc cggcgggccc tgaaggcagg gatcgcagaa gggggcggga
     8101 agtgatatcg ccggtgcgtc aggtcgtgga tatcggggaa gatgagccgg tcacgcttgc
     8161 cgaagcctgc cgcttgttct tcggtggccg ccttaagccg tcggcgttgc gcaccgaggc
     8221 ggcaaagggc aatctggaca tcatgcagat cgcccggaag gactttgtga ccaaacgcgc
     8281 gatcgaggag atgaaggagc gatgccgcaa aagcgcaagc cgccccgcct ctggctccga
     8341 acagaaacag gtagcgacgg acggaagcgc tccacctaca ttatcctcga cggcgcaaaa
     8401 catatccgca caggatgttc tgaggatgaa gctgaagaag caagccgcaa gctcgcagag
     8461 tacaccttca gcaaatacga ggccccacgg ggaggtcgtg cgtctgagat aacgatcagt
     8521 gacgtcctga tcgtctacct tgatgaaaag gccgatgcca ctgcacggcc caaggagacg
     8581 aaagcagcca tcaaccggct gaacgagttt ttcggctctg acaccatatc cgccatcaag
     8641 ggaaagcgct gccgcgactt cgtcgccgat cgcaagacgc aagctggcgc ccgtcgcgat
     8701 ctcgaagtcc ttcgggccgc ggtcggctac taccacaagg aatacggtct cgatgtcctc
     8761 ccggcgttca cactgccgga gaaggggcaa cctcgcgagc gatacctgac ccgatcggaa
     8821 gcggccgcgc tcctctgggc gtgccttggg tggcagaaag agaccatcgg cacgggcccg
     8881 aactcctatg aaaagtggaa gcggcggcgt tatcaccgcg cgaaccacct cgccaggctg
     8941 gtcttgatcg gcctctatac cggcacgcgc cccggcgcga tcaagcaact ccagtggatg
     9001 agaaacacga ccggaggatg ggcggatctc gaccgcggag tgatcttccg tcgagcggag
     9061 ggtgagcgcg tcgcccacaa taagcgcaag ccaccggtaa agctcgctcg ccgccttctg
     9121 gctcacatga agcgatggaa gcggatggac ggatgggagg atgaccaagt tgggctgcgc
     9181 tacgtcgtgc actattatgg aaagcccctg acgaaggaaa acaaggcgtt caggagcgcc
     9241 attgccgcgg cgggcctatc ttcagacgta acgccgcata tcctgcgcca cacgcgcggc
     9301 acatggctcg ctcaggcgca tgtgccgccg aacgaagctg cggcatcgct cggcctcacc
     9361 gtcgaagaat atgagcgaac ctatcttcac aatgatcccg acttccagaa agcggcggcc
     9421 gacgcctatt agaacatcgc aacggttcgc aatgtttccg caatgcttcg cggaacatgg
     9481 aatgaacaga cagcgatcaa cgatgatgag aaagcccgga tttgctggac tttctgcgag
     9541 cctacctttt tcgggacgag ggggtcgcag gttcaaatcc tgccactccg accagtaaaa
     9601 tcaaagagat agggaagacg acacttctca cttgctcccc ggcgcaatgc ttccgcaatg
     9661 aaacgactac gtaccccatg cgcctcggcc cctactccga ccattcgatc tggctcatat
     9721 tcgcgggcgt ggttctggcc gggattgtga tgtggtggat gggataacag cccagcgcta
     9781 ccttacaggc catggcacac cgcaagatcg tcgaaggcag cacgctcacg ctcgaggtga
     9841 cggtcatccg cgtctgggat gatggccgcg tcacattccg cctgtacgat cacgacgcgc
     9901 cgatcacgat accgggcgat gcgccgaaca tcatcctggt cagcgatccg gagaacgttc
     9961 cccggaaaaa gcaaaagccc cgccagcggt gagccagcgg ggccattcga gagttgctgc
    10021 cagcgttcga ggcgggttgg agtcaaacca actttgctgt cccagcgttt tcgtcggcgt
    10081 accgtactgg cttgcgctaa cgcacggtgc ccatcgttgg gcttctgccc cgacaactct
    10141 cgacagcctg tatatcatcc cacagggaaa taaaaaaagc cccgccggcc tctcagccga
    10201 gcggggcgac ttctttgcag aaaatgcata tcactcaggg cgggtcagtt cctcccgagc
    10261 atgcgcatga tcgtgtcgaa ataggtggtg atgagccatg tgagggctga ggcacccatg
    10321 cctacgatgg cgagggcgcc tataccgcgc tgtttccaag ccttcacctc ctccgtcacc
    10381 ttctgtacgt ccctgatcgt ccccttgatg ccggtcactt cgctttcgag actggtagtc
    10441 cgcaccacga gatcatccat gcgcttgtgc aaggcggcgc ggctttcgtc ttgccggtgg
    10501 agcctgtctt caatgctttc gagtttgccg atgacgacgc cgagggctct ttcaatgttt
    10561 gtgctggtcg gcaatgtccc cgctccttta atctcgctga aaccatggag cggtcaaaac
    10621 aagcgcagcc atggtcgaag atccgtttcg tccgtggtca ggctcggcgg gcggacccca
    10681 tccgcgcgcc gggctgctat ttgcagtagg catctcgggc ggcgttgctg ccgcgcacct
    10741 gccgtactgt ctcgggcgtg tcctgagacg ccgaataggt gatcggcagc catatcgagc
    10801 ataccgatgc cttaatgccg gtcgcggttg taggggtcac tgtctggcac cccgccaagc
    10861 tcagcgccag cacgagcatg attggcccga gcgatatcct ggagatggct ttcataggca
    10921 tccctttctt tggctgcggc ttcatttcgg ccggcctttc tgcctgcggc gaagatgcgc
    10981 cagacggcaa ggagtgccgc gacgatcccg gcaccgatgg ccgtgaggga accgccgggt
    11041 atcagggaga ggatgaagtc catcacacgt catccgctcg catccggcgg gcgatctcgg
    11101 tcacgatgcc ggagaagatc agccacagcg ggacatactt cggatcgatg tacggcacga
    11161 ccatctgagg atcgaccatg gcgatggcct gcagaacgat gccgaacaga acattgagcc
    11221 gggcccagag gatcgtttcc gagtcccgcg cccaggcgag cagcttttgc cacatgacag
    11281 ggttcctttc tcagttgcag aggatgccga gcagatcgca cggcagagag gcgagccatt
    11341 cccagaccat ggcggccgta gcggccaagg cgacaaggac ggccacaagc gcgcccgctt
    11401 tggcgtctcc gggctccgtg gagcgcgttt ccggggtctg aggctccggg acactgggcg
    11461 cgggcttttg cgcctgtgcg ggcttggcag gggccatttc gagagcgacg gcgcgcacag
    11521 cggcgacacg ggaggtccat cccttgtgga aggtgggcca gttcttcagc cctcgcagga
    11581 aagccatgcg cttgtcgcat aacgcttgga tgacatcggc cgcattggcg cgacgtgccg
    11641 cggcaagcgt ctgcgggccg attttgccat ccaccgccac gcggagcact tcctgaagga
    11701 actttgccgc ccgtgtcggg ccgctgttca ccgcgaagtc gaacaccgca tagtcgatgc
    11761 cgctcggcaa atcgtcgccc ttcaccttgt cccaatagtg cttgcgatag accttcgcga
    11821 cctgggca
//
LOCUS       DASVDU010000040         5097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_133320, whole genome shotgun sequence.
ACCESSION   DASVDU010000040 DASVDU010000000
VERSION     DASVDU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133320"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VF226_02890"
     CDS             <1..916
                     /locus_tag="VF226_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7072961.1"
                     /translation="IQLYMPLNFLGTVYREIRQSLIDLESMFDLLHENVEVEDKPGAP
                     PLDARRGAVAFEDVGFGYDPRRPILKDVSFRVEPGRTVAIVGPSGAGKSTISRLLFRF
                     YDVDEGRITIDGQDIRDVRQASLRAAIGIVPQDTVLFNDTVYYNIAYGRPDASPAEVE
                     EAARLARIHDFIMGLPDGYQTRVGERGLKLSGGEKQRVAIARTILKRPAVLLFDEATS
                     ALDTHTEKEIQKSLREVSANRTTLMIAHRLSTVIHADEILVLEAGRIVERGRHAELLA
                     RGGKYAAMWARQQEAAIEEGDRPFVEAV"
     gene            complement(1038..2315)
                     /locus_tag="VF226_02895"
     CDS             complement(1038..2315)
                     /locus_tag="VF226_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HEX7072962.1"
                     /translation="MFESLSPTTSQLVATVIFAVAVLHTFSTRTFERLAHLQPRHAGL
                     WHLLGEVEVVFGFWAMVLIVFLAVHDGPKDAMRYLEDQSFREAAFIFVVMVIAASRPV
                     LDLSAALLNWIGRLLPVAPPAAYYVTILSIGPLLGSFITEPAAMTLSAMLLRQRFFSK
                     QAPARFKYATIGVLFVNISVGGTLTPFAAPPVLMVADRWSWDFVFMLQAFGWKSALVV
                     VTNAVAATVLLREHLNQLPRGSKNLERVETPWPVVAVHLTLLGAVVIASHYTVLFIGL
                     FLFFLGFAEAYRRYQNGLILREGLLVAFFLAGIVVLGGKQEWWLQPLLAGMGSTTLYY
                     GAALLTTVTDNAALTYLGSLVDGLSAEARYALVAGAVSGGGLTVIANAPNPAGYSILK
                     GSFDQGVISPLWLVIAALPPTLVAVAAFQLLPG"
     gene            complement(2324..2680)
                     /locus_tag="VF226_02900"
     CDS             complement(2324..2680)
                     /locus_tag="VF226_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072963.1"
                     /translation="MTVVVLSSDEELRAAVAYWFEALSIPTVATADGYEANRALKSSV
                     AGLLITDRLLPPWPGLDSFRRLQSDNPRLCIAYVDDGSWDGAILARIAGATIVLPRPL
                     SRRQVVAALGLPEILA"
     gene            2911..4512
                     /locus_tag="VF226_02905"
     CDS             2911..4512
                     /locus_tag="VF226_02905"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7072964.1"
                     /translation="MAFPRIFILPNGVIGVAYLVLYVLLDWISFIDPFAAYGITPWNP
                     PTGLSFVLVLLFGQRMIPFLFVAPLLADLTVRQLPLSWALEFAAVSVIGAGYSLGLLF
                     LLRPKTRFNPSLPSLRDLLLLLLVAAASAVLVAVGYVGVVIVGGLLPPADFMSPFLRF
                     WVGDMIGVAIIAPFTLIALTRGQMLKMSLETAAQLVAVLGVLWLEFRFAQQNQLQFFY
                     ILFLPIIWMAIRTGLEGVTVGILITQLGLIAGVHLLPMQDVDITALQALMLVLTMSGL
                     MAGALVTERRRTELQLRLHQDSLAKLARLGSMGELAAAVAHEINQPLMAAGTYSRLVL
                     DALCRGAADEPKVVETAGKVVAQVERASEVLRRLRALVRLDKSGRAPTQLERIVNEAL
                     DLCRPDLDQNGIRVRVALESNLPPVLVDLLQVEQVLLNLMRNAIEAMTEAGQEGGTIT
                     IDAKQIDAGTVEVSLRDTGPGFPPELLADQFPPFSSTKVEGLGVGLSLSRSIVEAHGG
                     RLTTAGDRYGAVVRFTLPTATNDDA"
     gene            4505..>5097
                     /locus_tag="VF226_02910"
     CDS             4505..>5097
                     /locus_tag="VF226_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7072965.1"
                     /translation="MPENIRVALIDDDAAVLDSLRLYLEHNGVVVSSFSTAEAFLAIL
                     GSAGSVDCIVADVRMPGLSGIDLVREMQARNDTRPIILITAHGDVDMAVAAIKLGAFD
                     FLEKPFNEKRLLESIGMAVDQARHTQVQTADLEELRKRAEGLTDRQREVMELAAAGLS
                     NKEIAIRLQISPRTVENHRAWMMERMGASNLAELVRMV"
BASE COUNT          937 a         1615 c         1663 g          882 t
ORIGIN      
        1 gatccagctc tacatgccgc tcaacttcct cggcacggtc tatcgcgaga tccgccagtc
       61 gctgatcgac ttggaatcga tgttcgacct gctgcacgag aacgtcgagg tcgaggacaa
      121 gcccggcgcc ccgccgctcg atgcccggcg cggcgccgtt gccttcgagg atgtcggctt
      181 cggctacgat ccgcgccggc cgatcctcaa ggacgtgtcc ttccgggtcg agccgggccg
      241 gaccgtcgcg atcgtcggcc ccagcggcgc cggcaagtcg acgatctcgc gcctgctgtt
      301 ccgcttctat gatgtggatg aggggcgcat cacgatcgac ggccaggaca tccgcgacgt
      361 ccgccaggcg agcttgcgcg ccgccatcgg catcgtcccg caggacacgg tgctgttcaa
      421 cgacacggtc tactacaaca tcgcctatgg ccggccggac gcctcgcccg cggaggtcga
      481 ggaggcggcg cggctcgccc gcatccacga cttcatcatg ggcctgccgg atggctacca
      541 gacgcgggtg ggcgagcgcg ggctgaagct ttccggcggc gagaagcagc gcgtcgcgat
      601 cgcccgcacg atcctgaagc ggccggcggt cctgctgttc gacgaggcga cctcggccct
      661 cgacacgcac accgagaagg agatccagaa gagcctgcgc gaggtctcgg cgaaccggac
      721 gacgctgatg atcgcgcacc gcctctccac cgtgatccac gcggacgaga tcctggtgct
      781 ggaggcgggc cgcatcgtcg agcgcggccg gcacgccgaa ctgctggcgc gcggcggcaa
      841 atacgccgcc atgtgggccc gccagcagga ggccgcgatc gaggaaggcg accgtccctt
      901 cgtcgaggcg gtctgacgcc gcgcgcgggt agcggagggg gaccacaaag cgggccgtta
      961 tcccaatctg gggagccggc ccggcggaat actgcgcgtg tgaagcgcaa gtgatcttaa
     1021 ggtgagcttc gtcgcgttca gccaggaagc agctggaaag ccgcgacggc gaccaaggtg
     1081 ggcggcagag ctgcgatcac gagccagagg gggcttatga ccccctgatc gaacgagccc
     1141 ttcagaatcg aatagcctgc gggattcggg gcattggcaa tgacggtcag cccgccgccg
     1201 gaaacagcgc cggcgaccag cgcgtatctt gcttccgcag acaggccgtc caccagtgag
     1261 ccgagatagg tcagggctgc gttgtcggtg acggtcgtga gcagcgccgc cccataatat
     1321 aacgtcgtcg agcccatgcc ggccagcagc ggctgcagcc accattcctg cttgccgccg
     1381 agcacgacga taccggcgag gaagaaggcc acgaggagcc cctcgcgcag gatgagcccg
     1441 ttctggtatc gccgataggc ctccgcgaat ccgaggaaaa agaggaataa gccgatgaat
     1501 aggaccgtgt aatggctcgc gattacgacg gcgcccagca gggtgaggtg gacggcgacc
     1561 accggccaag gggtttccac ccgctccaga ttcttcgatc cgcgtggcag ctggttcagg
     1621 tgctcgcgca gtagcaccgt tgcggctacg gcattggtga cgaccaccag cgccgacttc
     1681 caaccgaacg cctgcagcat gaagacgaag tcccacgacc agcggtcggc gaccatgagg
     1741 accggtgggg cggcgaaggg ggtcagcgtg ccgccgacgg aaatgttgac gaacaggacg
     1801 ccgatcgtgg cgtatttgaa ccgcgcaggg gcctgcttgc tgaagaagcg ctggcgcagc
     1861 agcatggcgg agagcgtcat ggcagccggc tcggtgatga acgagccgag cagcggtccg
     1921 atcgaaagta tcgtgacgta ataggcggcc ggcggtgcca cgggaagcag gcgaccgatc
     1981 cagttcaaca gggctgccga aaggtcgagc accggccggc tcgccgcgat caccatgacg
     2041 acgaagatga acgcggcttc ccggaagctc tgatcctcaa gatagcgcat cgcatccttc
     2101 gggccgtcat gcacggctag gaagacgatg agcaccatcg cccagaagcc gaagaccact
     2161 tccacctcac ccagcaagtg ccagagaccg gcgtgccgag gctggagatg cgccaagcgc
     2221 tcgaaggttc gggtcgaaaa cgtgtgcagc accgcaaccg caaagattac ggttgcaacc
     2281 agctgggatg tggtgggcga taggctttcg aacatcgccg caatcaagcc aggatctcgg
     2341 gcagcccaag cgccgcaacc acctgccggc ggctcaatgg ccggggcaga acgattgtcg
     2401 ccccggcgat gcgggcgaga atcgccccat cccagctgcc atcgtctaca taggcgatgc
     2461 acaggcgcgg attgtccgat tgaagccgac gaaacgaatc gagacccggc cagggcggca
     2521 acaaccggtc cgtgatcagc aacccggcga cgctgctctt cagcgcccgg ttcgcctcat
     2581 atccgtcggc ggtcgcgacg gtgggaatcg acaaggcctc gaaccagtaa gccaccgcgg
     2641 cccgcagctc ctcgtcggaa ctcaagacca ccactgtcac cggttggctt cccggacagt
     2701 cgtttcgctg gacgataggc atgaagcacc ccaaaaggcg tgatcttcgt gccattaggt
     2761 ggattcccgg tcggcccggg caattcggat gcgtccctag ggatcgccgc ttgggtgggt
     2821 atacttaggg gttcttactc agggctctcg gcaaaaggtg gcgatagact tatgggcagc
     2881 gtcaggcccc aacatcaccc gaaccgcccg atggcctttc cgcggatctt catcctgccc
     2941 aacggcgtca tcggcgtcgc ctacctggtg ctctatgtcc tgctggactg gatcagcttc
     3001 atcgacccat ttgccgccta cggcatcact ccgtggaatc ccccgaccgg tttgagcttc
     3061 gtgctcgtgc tgctgttcgg ccagcggatg atcccgttcc tgtttgtggc accgctttta
     3121 gccgatctga ccgtcaggca gctcccgctt tcctgggcgc tggaattcgc tgcggtctcg
     3181 gtgatcggtg cgggatactc tctgggattg ctcttcctgc tacggccaaa gacgcggttc
     3241 aatccgtctc tgccatcgct acgcgacctg ctccttctgc tgctcgttgc agcggcaagt
     3301 gccgtcctgg ttgcggtggg ctatgtgggc gtcgtgatcg tcggcggctt gcttccgccg
     3361 gccgatttca tgtcgccttt cctgcggttc tgggtgggtg atatgatcgg cgtcgccatc
     3421 atagcaccgt tcaccctgat cgcgcttacg cgggggcaga tgctgaagat gtcgctggaa
     3481 acggcggcgc agctcgtcgc tgtgcttggc gtgctgtggc tggagttccg gttcgcgcag
     3541 caaaaccaac tgcagttttt ctacattctg ttcctcccga tcatctggat ggccatccgg
     3601 acggggctcg agggcgtgac cgtcggcatc ctgattacgc agctcggtct gatcgccggc
     3661 gtgcatctgc ttccgatgca ggacgtcgac attacagccc tccaggcgct gatgctcgtg
     3721 ctgaccatgt ccggcttgat ggccggagct ctggtgaccg agcgccgacg gacagagctc
     3781 cagctgcgcc tgcaccagga ctcgcttgcc aagctcgccc gccttgggag catgggcgaa
     3841 ctggcggccg ccgttgccca tgagatcaac cagccattga tggcggcggg gacctattcc
     3901 cggcttgtcc tggatgcgct gtgccgtggg gccgccgacg aaccaaaggt cgtggagacc
     3961 gccggaaagg tggtagcgca ggtggagcgt gcatcagaag tgctcaggcg gctgcgcgcg
     4021 ctggtccggc tcgataagtc cggtcgggct ccgacccagt tggagcgtat cgttaacgag
     4081 gccctcgatc tctgtcgacc ggatctcgac cagaatggca tccgtgtgcg tgtcgccctt
     4141 gaaagcaacc tgccgccggt cctcgtggat ctcctgcagg tggagcaagt tttgctcaac
     4201 ctgatgcgga acgcgatcga agcgatgacg gaagcggggc aggagggcgg gacgatcacc
     4261 atcgacgcaa agcagatcga cgcgggaacg gtcgaagtca gcctgcgcga caccgggcca
     4321 gggttcccgc cagagctcct ggccgaccag ttcccgccct tctcctcgac caaggtcgaa
     4381 ggcctcgggg tcggtctctc gctgtcccgc tcgatcgtgg aagcgcatgg gggtcggcta
     4441 accactgcgg gagataggta tggcgctgtc gtccggttca ccctgcccac ggccacgaac
     4501 gacgatgcct gagaacatcc gtgttgccct gatcgacgac gatgcagcgg tgctcgactc
     4561 gcttcggctt tacctcgaac acaacggcgt cgtggtatcc agcttttcga cggcggaggc
     4621 cttcctcgcg atactgggct ccgcgggctc tgtcgattgt atcgtggcgg acgtccggat
     4681 gcccggcctg tccggcatcg acctcgtgcg ggagatgcag gcgcgcaacg acacgcggcc
     4741 aatcatattg attaccgcgc atggcgatgt cgacatggcg gttgccgcga tcaagctcgg
     4801 cgccttcgat ttcctcgaaa agccgttcaa cgaaaagcgc ctgctcgaaa gcatcggcat
     4861 ggcggtcgat caggcgcgcc acacccaggt acagacagcc gacctcgagg agttgaggaa
     4921 gcgagccgaa gggcttaccg accggcagcg ggaggtcatg gaactcgcgg ccgcaggact
     4981 gtcgaacaag gagattgcca tccgcctcca gatcagccca cggaccgtcg agaatcatcg
     5041 cgcatggatg atggagcgca tgggagccag caatctcgcc gaactcgtcc gcatggt
//
LOCUS       DASVDU010000041        19668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_904769, whole genome shotgun sequence.
ACCESSION   DASVDU010000041 DASVDU010000000
VERSION     DASVDU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19668
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904769"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..564
                     /locus_tag="VF226_02915"
     CDS             325..564
                     /locus_tag="VF226_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072966.1"
                     /translation="MSTPTTYTISDTPMSRGKSNGLLQRLFDRLLAAREAQAHRYLVD
                     YLSNMSDARLAALGYTDEQIRQIRIERRLPELAAA"
     gene            complement(887..1804)
                     /locus_tag="VF226_02920"
     CDS             complement(887..1804)
                     /locus_tag="VF226_02920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX7072967.1"
                     /translation="MPSYKITIAVDEQEMARTLTGALENLIEPSPDALTQFEAGEGWL
                     IEGYYQERPNLAALTRELGAALPFSPPPIGIEEVPDENWVAISQAALPPVSAGRFTVH
                     GSHDLGKVPRGPNTLLIDAGEAFGTAHHATTQGCLLALDELTRRRSFDRVLDLGCGSG
                     VLAIAAARALPNARIEASDSDPQAVAVACTNARLNGEAGRIRLRVAEGLARGRTRAAS
                     YDLILANILADPLVALAPDLAHALAPSGIAVLSGLLERQAAYVIAAYRSHGFSLLSNR
                     RLAGWSTLVLIRGPRAADACPYAPPRPAA"
     gene            complement(1891..3357)
                     /locus_tag="VF226_02925"
     CDS             complement(1891..3357)
                     /locus_tag="VF226_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM143 family protein"
                     /protein_id="HEX7072968.1"
                     /translation="MSAVPSNASRVQLRDERTSREPASDGEAGSYPLNGHGNEELEPD
                     AIADERERFIPVTRFALIDRLTEPHIWPNGQAADARRFFRYLDYWRQQQYGARLLGLE
                     QDYEPFSPDSDLLMTRSYTEEERRLLQQRVVAGIDSLLKQANYTRINPQQVELILTRE
                     SHYGLDLHVDLDAFEELLIYYRGATTRTHHRRSLKSFLRKQEIDVPIFRRLFVLFKLK
                     SEEQRIEELVRERGVSRGAASRAVRRMRRLLAPEVRDDLIYMKLFKNIPRSDIEMIFP
                     NTRVRFRPFDKLKLGITSSAGVGMGAFSAAGKLALLTTNPLAAAGAVFGLGGIIFRQC
                     RRFLNERQRYMVIMAQNLYFHAMADNRGVLIKLASRAAEEDMKEEILLYAVLAKGPVR
                     RSDLPEVDRVIERYMEETFGVDVDFEIEDALERLIAEGIVSEAADGTLHTLPPAEAAR
                     LIDRKWDAFLDLLPGSGEVEGTEMRDGNVVQANVSATA"
     gene            3671..5458
                     /locus_tag="VF226_02930"
     CDS             3671..5458
                     /locus_tag="VF226_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX7072969.1"
                     /translation="MQSQIMPRLFGFALLLVLGVGNAVARDAVVLPASCEFRAGRLIL
                     HASQPTAHEIVSRRERQVFTACRAGGDNRCRRVMVYKFDLACGGGTVSWSDVSAALMA
                     ASGTRAWVRNEQVHIVPPRASRLGECEGAGPDKSFMRECEPWEVAESNRTIWVLPQRF
                     APVHEVGARFIELPPATASAEQNRPLPPSVGSSSGELQIAEKPDEDEVSPADASNAQQ
                     EQFERVYEAYDAPPPVPAPTQADRPRRADSLELSTGAFRRADGPVTEGVQSSEADGVQ
                     TFARSVSQAIAAAPPENVRVAELDQHEPASSLREWSLPDWSFSINLADNKLTSWLWDQ
                     ANATLAPLSGHLKDLRSSQQFTAVLMGLALLSGLLSGIGWYSARSRRARASAFRASHD
                     QPTPEFRAATSSGPAFSAGPSSVSKVATGTALLAPTDEKMCGELCRTAQSMLQQIDAR
                     MDELQGVAPLRRVLQREMRNLEQFLTAVMTASPAEPEEWRRMRNRLQRIVRELHRLRD
                     IVEGAYRSLSGGGFTSREPPRDKYEAYEALGVNPDVSMRTLKKLVDALRACWHPDLAK
                     DEADRQLREERMKRINIAWDIITSKRQEA"
     gene            5481..5951
                     /locus_tag="VF226_02935"
     CDS             5481..5951
                     /locus_tag="VF226_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7072970.1"
                     /translation="MAGRTPQKPAKAAKKPAAKPASGEPVLLTGGNPQIPKGYGDAPV
                     QAYIAAMPGWKRDVGRCLDEIIARTVPGVRKAVKWNSPFYGVEDNVWFLSFHCFTRYV
                     KVAFFRGASLNPMPPGTSKQKDVRYLDIFEGDEFDEAQFADWVRQAAALPGERM"
     gene            5971..6792
                     /locus_tag="VF226_02940"
     CDS             5971..6792
                     /locus_tag="VF226_02940"
                     /inference="COORDINATES: protein motif:HMM:NF010309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase family
                     protein"
                     /protein_id="HEX7072971.1"
                     /translation="MIPALANEPESHAQIAATVDIVRDVLGEAVCAACLYGSAVAGGL
                     RPSSDLDILVVSNQSLNSDAQAAIIRQLLPISGRHGIRGSARSLELSIVARSALVPWR
                     YPPSIELQYGDWMRAEFERGKLPEWPKADPDVAVLVETARRASVPLFGEPISAVLEPV
                     PRADLVRAMRDTVPVLMPGIEEGDDVRNGLLTLARIWTTLATGEIRPKDEAASWALTR
                     LPEEHRAVLAHARATYRGETPENWSALGPRLRPHVDYVVARIDELASVQVSNGQS"
     gene            6809..7264
                     /locus_tag="VF226_02945"
     CDS             6809..7264
                     /locus_tag="VF226_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7072972.1"
                     /translation="MKRKIAAKEGEEGNSPSQLVDAKIKGVTDWRDAMLARIRQLIKE
                     ADPEVVEEVKWRKPSNPAGVPVWSHDGIICTGETYKDKVKLTFAKGASLEDPTGLFNA
                     SLDGNVRRAIDLHEGDKIDEDAFKALIHAAVALNTRAARPRSQKRAKSA"
     gene            complement(7454..7963)
                     /locus_tag="VF226_02950"
     CDS             complement(7454..7963)
                     /locus_tag="VF226_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7072973.1"
                     /translation="MVFTRKPEPRSGNVDPLQIKTMDHQATGTPTPTPMRSNGFSGTS
                     GQDADESVIGNDLTIEGQAITIRCQGSLRVNGNIQADLHSMKLTVGEEAVINGTIAAE
                     TVDVYGKVQGAILGSRVVLHESAVVEGDIHSQALAIDQGASFDGRSRKVKDPSEIAPQ
                     LTSPNRTTS"
     gene            complement(8175..10574)
                     /locus_tag="VF226_02955"
     CDS             complement(8175..10574)
                     /locus_tag="VF226_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7072974.1"
                     /translation="MQSNRKHQMQGEDALAPAARKEGKASLEPDLPISQRALRAAEPP
                     YLAGLNEAQRQAVETLDGPVLVLAGAGTGKTRVLTTRIAHILATGRARPSQILAVTFT
                     NKAAREMKERIGQLIGGAVEGMLWLGTFHSINARILRYHAELVGLKSSFTILDTDDQI
                     RLMKQVIEAGGVDKDRWPARQLAALIDSWKNRGFGPAQVPAGESFAFANGKGAALYAD
                     YQKRLKELNAVDFGDLLLESLRLLREHPDVLADYQRRFRFMLVDEYQDTNVAQYLWLR
                     LLAQGSASPNLCCVGDDDQSIYGWRGAEVENILRFEKDFPGAKVIRLERNYRSTGHIL
                     GAASQLIAHNRGRLGKTLHTDDGLGEKVIVTGVWDDEEEARIIGEEIEKLQQAGQPLN
                     DIAILMRASFQMRAFEDRFMTLGLPYRVIGGPRFYERQEIKDAIAYLEVTLNPANDLK
                     FERIINVPKRGLGDTSLKRIHALARATDMPLYHAARSITETEEITGKARKSLMELINS
                     FERWRGLVPSMKHTELAELILDESGYTAMWQQDKSPQAQSRLENLKELIRFMHEFDSL
                     AGFLEHVSLVMDTDQADTGDRVSLMTLHAAKGLEFDVVFLPGWEEGLFPHQRSLDENG
                     QAGLEEERRLAYVGLTRARRHVQVSFAQNRRMRGLYQAAVPSRFVDELPPQHVEVVEA
                     PSRFGGAYSAFDRMANPYGRSRFDDVGSFQTNYQTPGWQRAQQRWQQGHQPKRGPVVI
                     EGELVASSTGQPSSYNVGERVFHQKFGYGEVAEVDGNKLTVDFDKAGRKRVVDSFVDR
                     V"
     gene            10840..14670
                     /locus_tag="VF226_02960"
     CDS             10840..14670
                     /locus_tag="VF226_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX7072975.1"
                     /translation="MNGALLWIAGLIVVLLAALFAVPYAVDWNAYRGVFETEASRMLG
                     REVRVGGDVSLRLLPAPYISFEKPRIADATAGEAFFRADSFTMWLALPPLLQGIVEAR
                     QVEIERPVLRLQIHEDGGGNWRTFKISRADLPFVPRGLTLQSVKITDGIIAVHGVSGE
                     PLAKIEIAEGELSAPALEGPYRLRADVRWNDDIREVRASTAPPDPDGSTRLKLAVRAP
                     KSGNAYTFDGRIVDLVGRPQFDGSFTATIPVTSGTGELRPTTVQSADSEAIPPAALHS
                     GVFEVRAAVSANAVSAKLSDLGFSFEQNGQPQLLTGTAEASWLQKPVIRATLTSRWLD
                     LDRIADVPSNSNALQALRSLASSVTGFLPTQADVAARLTIDQATLAGDMVSNLVVAIE
                     NAGAGLTIKEFHAALPGGSRVDVTGALTGSGTAEAFDGEILVRGANLNRFLAWAARGT
                     QFADAPSDAGFALSGQLSLGPGRLELSKASIDSGPNRLEGEITYRWDGRRNLVLSLET
                     SRADISGVLPGVLGPDLLKAKLGALTGSDGGNLNSLLAGLAGIDLSMHIRADALTDGA
                     RMLHNVDADVRLEDGRLRIPVFRASTPEGFKLELDGDVRDFAGEPKGALQGTIEAPTR
                     AALSEALDLAADDIDGERRQWLAALAPLRLAFVSRFGQPPGERVVMTIDGTSHGEHLV
                     ATLVLEGGLAEWRSKPADLMLTSESNEVARVVRGLLLGGTEEREARSTLPKGRLLVKA
                     AGVPEKDAAIFVQMIAGDDLQLLLQGRGALPANGSPTFKGEMEVAAENSGRALHFAGI
                     PLPANAVEGGLKGRAAVTVADHALTFALDRFEVGKVRLSGEAKLGGTSETRTLDLDLK
                     ADSASLPRLLALALDTEIQTSEIPADIAQLSQWQDQPFDFSNLEHVNGSLNLKADTLA
                     LGQGFELADAVLEAQIEPGRITITKLEGRALGGAVSGAFKLEKTAAGGQMSGAFGLWD
                     IRLDHIKGASNAPIGAGSLRLTLQVNGQAVTPRALIPVLTGKGELQFRNARWERLSAK
                     AIEAAADAVLSGDTAPTGEPLRQALRTALAREPLLLGDLKIPVVVGAGALRIDGFTVE
                     TPEAQIINRTTIDLTEVAIDSEWKLQPKATRGGGTPLPGISVVYVGPLKSIASLEPQL
                     FIDMLERELAVRNMERDVEHLEQLRREDEARAKAEAERLRKLEEERLMQFQELQGAQG
                     LTIDVPFPFPASPSTAPAPQSLTIEPETPSQHPQPAAPQQRITPQWDGQWPPPERSGT
                     SAPASSAPEVRRAL"
     gene            complement(14595..14888)
                     /locus_tag="VF226_02965"
     CDS             complement(14595..14888)
                     /locus_tag="VF226_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX7072976.1"
                     /translation="MQPAKSASVRPLKRSFTIAGHSTSISLEAPFWEALKEAAAERGQ
                     SLAALVEEIDASRGEAGLSSAIRVWILGYYRARRTSGAEDAGAEVPERSGGGH"
     gene            complement(14888..15604)
                     /locus_tag="VF226_02970"
     CDS             complement(14888..15604)
                     /locus_tag="VF226_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072977.1"
                     /translation="MAGPILSVEEVDLGYGNLQVVFGVTLHVEQGELVGLVGGNGSGK
                     STILRAISGMISPWRGRIVFDGEEINRVEPHQMAEKGLAHVPMGRQLFPNMTVYDNLL
                     LGAYLPRAKAQREENLNRVGQLFPDLLAHYDEPAGQLSGGQQQMVAIARALMLGPKML
                     IMDEPSLGLSPILVKHVMAAIRRVAETGVPILLVEQNVKQVLNVTHRTYVLENGRLVL
                     EGPSTELQDHPMIRKAYLGL"
     gene            complement(15604..16320)
                     /locus_tag="VF226_02975"
     CDS             complement(15604..16320)
                     /locus_tag="VF226_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7072978.1"
                     /translation="MPEVLRAERLTVRFGGVTAVDHVDLKLADRDILGLIGPNGAGKT
                     TLFNALTGFVRPDEGAVFMFGREVTHLAPFQRARDGMGRTFQTDRPFEELTVLENVLI
                     PAFLKHPSRRSAERIAREVLDEVGLSDRADQPAMDLNLARRRRLELARALAIEPRILF
                     LDEIMAGLNPPALREMISFVRKLREKGLAILMVEHIMEAIIELCDHVIVLANGEKIAE
                     GAPQQVVADSRVVEAYLGTD"
     gene            complement(16298..17251)
                     /locus_tag="VF226_02980"
     CDS             complement(16298..17251)
                     /locus_tag="VF226_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072979.1"
                     /translation="MNTNLFGLALLLVIGLAYPFVLPGYLTVGITMLLFIGWATSWDI
                     LGGWAGQVSLGHSFFVGLGAYFVALGQSRYGLAPWWSIMLAILAAAALAYVWGRITFG
                     LRGPYFALSTIAFAEIFRLIAINEKWLTGGATGVFIASLPEPFGIDLFSRWTQYYLAL
                     AFALITVGIVALISTRRFGYQLRAVREDENSAMASGIDPTAVKLKAFMLSGALTAVGG
                     GIYGIFLSFLEPHVLFYLLLSVQIALTAIIGGRGTVWGPAAGAVLLILSGEVFRTTFA
                     QANLLIYGMLILAIVLFLPRGILGEVSRRLVRRRYARSAKS"
     gene            complement(17248..18135)
                     /locus_tag="VF226_02985"
     CDS             complement(17248..18135)
                     /locus_tag="VF226_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7072980.1"
                     /translation="MADLIPFLQNIANGLLIGGVYALIGVGLTLIFGVMRTINFAHGE
                     FVVLGMYAAIVFNQALGWDPYFSLVVALPAGFLAGVLIQRVILATIVDAPEESSLLAT
                     LGLSLIIANSLLLIFGGEPKSVLVPYASSTISLGGGVNLSIVLLLAGIATVAAIGGLY
                     LLLNRTEIGRAIRGTAENRLGAELVGINTKRIHAVVFGIGMMLAVAAGVALIPLLFAS
                     PTTTGTVFTLKAFVVTVLGGLGNIGAAIGGGLLLGVVEVLGASYLTSEYRDAYGLVAF
                     ILVLLFRPEGLFGRSVQRV"
     gene            complement(18248..19438)
                     /locus_tag="VF226_02990"
     CDS             complement(18248..19438)
                     /locus_tag="VF226_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072981.1"
                     /translation="MKRLTLIAAAAAAVAMTSPGAHAQVKVGVVNSLSGAFAAFGQRY
                     KTGMEVALEEINSSGGINGQKLEITVQDDRSEAQSALAAVESFVNQGVPLIIGSYASS
                     ITGPMAQLATRQKVPLVVLGSADDSITKPGSPWVFRAKHNSTIVARTYFDYFDFLRKK
                     GDTELKTVAMLYGNGAWPTSLAEAGKKFAKERGYEVIGDQSYDQGVTDFRPILNRFRG
                     QQPDILYIVSYADDGIAITRQMREVGLSADVIAIDTSAALPSFIEQVGDLSEYVATVV
                     SWSKDVKYEGVKDLYERMKAAAGSEPSFYEAEGYLALRVAADALRRAGSTDREAVREA
                     LSKTDLQTPVTTVRFESFDGFQNQNPIRSLVLQIQDGQHVTVFPEDLAAKEVRHPTPP
                     WDKR"
BASE COUNT         3630 a         6365 c         6229 g         3444 t
ORIGIN      
        1 tccaccccga gctgcgccat caaagcacgg agttgtttca cgcggtcgac cgcgttttcc
       61 aggccggtgg gggcttcgaa gctctgaaac atcatgatct ccgggctcga ctcaggccaa
      121 ttcgcgcgcg ctccgtacac gactcgaatc gattgtgacg gtcgttcgaa gcaacgtatc
      181 gatcagcatg cgccgaccgc attgctgcat tgcacactca gtctaccgca agtgcgaagg
      241 agagactata taactgttaa ccgctgggga gggcggcgct cagaccggcc cctcagcaaa
      301 aacactagca gagatgagct gaccatgagc acaccaacca cttacaccat ctcagataca
      361 cccatgtcga gaggcaagtc gaatggcttg cttcagcgcc tgttcgatcg gctccttgcc
      421 gcccgcgagg cgcaggccca tcggtatctg gtggattacc tctcgaacat gagcgatgcc
      481 cggcttgctg cgctcggcta cacggacgag cagatccgtc agatccgcat agagcgccgt
      541 ctgcccgagc tggcagccgc ctgaaccact ccacttccca caggcacccc gtcgtcactt
      601 cggctgggaa atcaccccca gccgaggtgc tgtttgccga gaggacatga gatcatgagc
      661 acgtcatcac attccacggc gctgaacgcg cacacatcag tcaacgccga ccgggaacca
      721 gtcggcctcc gtctacggca gctgctttca accatactga agcggattcg ggccgcggca
      781 gaggaatatc gggtacgcca gcatcttgcc actctacccg actcgatgct gcgcgacatc
      841 ggcatcgcag aagacgagat tcatcgaatt cgggcctgcg agcgattcac gccgcgggcc
      901 tgggcggagc gtatgggcac gcatcggcag ccctgggacc tctgatcagc accagggtcg
      961 accagccggc cagacgccgg ttgctcaaca gcgagaagcc gtgtgaacgg taggcggcga
     1021 tcacataggc cgcctgccgc tccaacaagc cagacaggac ggcgatgccg cttggcgcga
     1081 gcgcatgcgc cagatccggc gcgagcgcca ccagcggatc agccaggata ttcgccagga
     1141 tcagatcgta gctagccgcg cgggtgcgcc cgcgtgcgag accttcggcc acgcggaggc
     1201 ggatgcggcc ggcctcgcca ttgagccgcg cgttcgtgca cgcaacggca accgcctgcg
     1261 gatcgctatc gctggcctcg atccgcgcat tcggtagagc gcgcgccgcg gcgatggcca
     1321 atacgcccga gccgcaaccg aggtccagca cgcgatcgaa gctccgccgg cgcgtcaact
     1381 cgtcgagggc aaggaggcac ccctgcgtgg tcgcatggtg cgccgtccca aaggcctcac
     1441 ccgcatcgat cagcagtgta ttgggtcccc gcggcacctt tccgaggtcg tggctgccgt
     1501 ggaccgtgaa gcggcctgcg ctgaccggcg gcagggccgc ctgcgagatc gcgacccagt
     1561 tctcgtcagg gacctcctca atcccgatcg gcggaggtga aaacggcaga gccgcaccga
     1621 gctcccgggt caatgcagcc agattgggcc gctcctggta gtacccttcg atcagccagc
     1681 cttcaccggc ctcgaactgg gtgagcgcat cgggtgaggg ctcgatcagg ttctccagtg
     1741 ctccggtgag ggtgcgcgcc atctcctgct catcgacggc aatcgtgatc ttgtatgatg
     1801 gcatggcctg ttccgaagcg ccccgcaggg cacgatggct tctggcgcgg acctatcctg
     1861 caaaagctgt tttgtcgatc gggcaaaccg tcaggccgtg gcgctcacat tggcttgcac
     1921 gacgttgccg tcgcgcattt ccgtcccttc cacctcgccc gagccgggca gcaggtccaa
     1981 aaatgcatcc catttccgat cgatgaggcg cgccgcctcc gcaggcggaa gtgtatgcag
     2041 cgtcccgtcc gctgcctcgc tgacgatgcc ttccgcaatc agtcgctcga gcgcgtcctc
     2101 gatctcgaaa tcgacgtcga cgccgaacgt ctcctccatg tagcgttcga tcacccggtc
     2161 cacctcgggc aggtcggagc ggcggaccgg ccccttggcc aggacagcgt agaggaggat
     2221 ctcctctttc atgtcctcct cggcggcgcg gctcgccagc ttgatgagaa cgccgcggtt
     2281 atccgccatg gcatggaaat agaggttctg ggccataatg accatgtagc gttgccgctc
     2341 gttcaggaac ctcctgcact gccggaagat gatgccgccg agaccgaaaa cagcccccgc
     2401 cgccgccaga gggttggtcg tcaggagcgc cagcttgccc gcggcactga aggctcccat
     2461 gccaacgccg gcgctgctcg tgatgcccag cttgagcttg tcgaacggcc ggaatctcac
     2521 ccgggtgttg ggaaagatca tctcgatgtc cgaccgcggg atgttcttga acagcttcat
     2581 gtagatgagg tcgtccctca cctccggcgc cagaaggcgg cgcatccggc gcacggccct
     2641 gctcgcggcg ccgcgtgaga cgccccgctc gcgcacgagc tcttcgatcc tctgctcttc
     2701 cgacttgagc ttgaacagca cgaacaggcg ccggaatatc ggaacgtcga tctcctgctt
     2761 gcgcagaaac gatttcaacg agcggcggtg atgcgtccgg gtggtcgcgc cgcgatagta
     2821 gatcagcagc tcttcgaagg cgtcgaggtc cacgtgcagg tcgagcccgt agtgagattc
     2881 ccgggtcagg atcagctcga cctgctgcgg attgatccgc gtgtagttgg cttgcttcag
     2941 gaggctgtcg atgccggcca ccacgcgctg ctgcaggagc ctgcgttcct cctcggtgta
     3001 gctgcgggtc atcagcagat cgctgtcagg gctgaagggc tcgtagtcct gctcgaggcc
     3061 cagaaggcgg gcgccgtatt gctgctggcg ccagtagtcc agatagcgga agaaacggcg
     3121 cgcatccgcc gcctgaccat tcggccagat atgcggctcc gtcaggcgat cgatgagcgc
     3181 gaaccgcgtg accggaatga accgctcccg ctcatccgcg atcgcgtccg gctcgagctc
     3241 ctcattcccg tggccattga gggggtagga gcctgcctct ccatcagacg ccggctcgcg
     3301 gctggtcctt tcatcccgaa gctgaacgcg gcttgcgttc gatggcacgg ccgacatgag
     3361 gaacccctcc ccgatctctc tcgaatcctt accagctggc cgcccttgtt ctttacgaga
     3421 ggtgcgccac ggcagaaagt cttcttgaaa ttgtcggctt gacggcgcga caggaacctg
     3481 cggcatcaga ataatccggg ttcgaccctc tgcgcgagtg cttgtgctag cagagttttc
     3541 tttgatctga gcataagaaa tccgtgcggc cgttaggcaa catttgtaaa atccgttgca
     3601 atgtcacagt catgccgctc ttgccgatca gataagctgg atgctgtcct gcggggcatg
     3661 ggggctgctc gtgcaatcac aaatcatgcc gcgcctgttc ggttttgcgt tgctcctcgt
     3721 gctgggcgtg ggcaatgcag ttgcgcgcga tgccgtcgtc ctgcctgcga gctgcgagtt
     3781 ccgtgcgggc cggttgatcc tgcatgcctc ccagccgacg gcgcacgaga tcgtctcgcg
     3841 gcgggagcgc caggtcttca cagcctgcag agctggtggc gacaatcgct gccggcgtgt
     3901 gatggtttac aagttcgacc tggcctgcgg tggcgggacc gtgtcgtgga gcgatgtttc
     3961 cgcggctctg atggcagcca gcggcacccg cgcgtgggtc cgaaacgagc aggtgcacat
     4021 cgtaccgccg cgggcgagcc ggcttggcga atgtgagggg gccggccccg acaagtcgtt
     4081 catgcgcgag tgcgagccct gggaggtcgc cgagtcgaac aggacaatct gggtcctccc
     4141 gcagcggttc gctcccgtgc atgaggtcgg cgcgcggttc atcgagcttc ccccggcgac
     4201 ggcctcggct gagcagaacc ggccgcttcc gccttcggtc ggctcatcct ctggtgagtt
     4261 gcagatcgca gaaaagcccg acgaggatga ggtttcccct gcagacgcct cgaatgcgca
     4321 gcaagagcaa ttcgagcgcg tgtatgaggc ctatgacgcg cccccgccgg tccctgcgcc
     4381 gacccaggcc gaccgcccgc ggagagctga ctccctggag ctttccaccg gcgctttccg
     4441 gcgggccgac ggtccggtga cggaaggcgt ccagagctcc gaggcagacg gcgttcagac
     4501 gtttgcacgc agcgtttcgc aggccatcgc agccgcgccg ccggagaacg tgcgggtcgc
     4561 cgagctggac cagcacgagc ctgcttcgtc gctgcgagag tggtcgttgc ccgactggtc
     4621 gttctccatc aaccttgcgg acaacaagct gacatcctgg ctctgggatc aggccaacgc
     4681 cacgctggcg ccgctctccg ggcatctcaa ggatctgcgt tcgagccagc agttcaccgc
     4741 cgttctgatg ggacttgccc tgctatccgg tctgctttcc ggcatcggct ggtattcggc
     4801 gcgttcgagg cgagcgcggg cgtcggcgtt ccgggcctct cacgatcagc caacccctga
     4861 gttcagggcg gcgacgagca gcgggccggc cttcagcgcc ggaccatcat ccgtcagcaa
     4921 ggtggcgacg ggaaccgcgc tgctggctcc gaccgacgag aagatgtgtg gcgagctctg
     4981 ccggacagcg caatcgatgc tgcagcagat cgatgcccgg atggacgagc tgcagggtgt
     5041 cgccccgctg cggcgtgtgc tgcagcggga gatgcgcaac ctcgaacagt tcctgacggc
     5101 cgtgatgacg gcgtctccgg ccgagccgga ggaatggcgc cgcatgcgca accgcctgca
     5161 acggatcgtg cgcgagctgc atcggctcag agatatcgtc gagggcgctt atcgcagtct
     5221 ttcgggcggc ggcttcacca gccgcgagcc gccgcgtgac aagtacgagg cctacgaggc
     5281 gcttggcgtg aatcccgacg tcagcatgcg gacgctgaag aagctcgtcg atgctctgcg
     5341 cgcctgctgg caccccgatc tggccaagga cgaagcggac cggcagctcc gcgaagagcg
     5401 catgaagcgg atcaacatcg cctgggacat catcacctcg aaacgccagg aggcgtgagc
     5461 caatcgcgag aagtagcgtg atagcgggca ggacgcccca gaagccggcg aaagccgcga
     5521 aaaagcccgc agccaaaccg gcttccggag agccggtcct cctcacgggc ggcaatcctc
     5581 agatccccaa gggctacggc gacgctcccg tacaggccta cattgcggcc atgccaggct
     5641 ggaaacgtga cgttggacgc tgcctcgacg agatcatcgc gcgcacagtc cctggcgtgc
     5701 gcaaggccgt caaatggaac tcgccgttct atggcgtcga ggacaacgtc tggttcctca
     5761 gctttcattg cttcactagg tacgtgaagg tggcgttctt ccgcggggcg tcgctcaatc
     5821 cgatgccgcc gggcacgtcg aaacagaagg atgtgcggta tctggacatt ttcgagggtg
     5881 atgagttcga tgaggcgcag tttgcggatt gggtgaggca ggctgcggcc ctgccggggg
     5941 agaggatgtg agaggccaga tttcgcggtc atgattcccg cccttgcgaa cgaaccagag
     6001 tcgcacgccc agatcgcagc cactgtcgac atcgtccgcg atgttctcgg cgaggccgta
     6061 tgcgcggcct gcctctacgg gtccgccgtc gcgggcgggt tgcgtccgag cagcgacctc
     6121 gacattctcg ttgtttccaa ccaatcgctg aacagcgacg cgcaggccgc gattatccgg
     6181 cagctcctgc ctatttccgg acgacatggc atccggggct cagcccgctc gcttgaactt
     6241 tcgatcgtcg ctcgctcggc gctcgtgccg tggcgttacc caccgagcat tgaactgcag
     6301 tacggcgatt ggatgcgcgc cgaattcgag cggggcaagc taccggaatg gccgaaagcc
     6361 gacccagacg tcgcggttct tgtcgagacg gctcgccgtg cgtccgtgcc actgttcggc
     6421 gagccgattt ctgctgtcct ggagccggtg ccgcgcgccg atctcgtccg ggccatgcgc
     6481 gacaccgtcc cagtcctgat gccgggcatc gaggaggggg acgacgtccg gaacggactg
     6541 ctcacgctgg cgcgcatctg gacaacactc gcgaccggtg agatccgccc gaaggatgaa
     6601 gcggcgagct gggcacttac tcgcctcccg gaggagcaca gggccgttct tgcacacgct
     6661 cgagccactt accgcggcga gacgccggag aattggagcg cactcggccc gcgattgcga
     6721 ccgcacgtgg attacgtcgt tgcacggatc gatgaactcg cgagtgtgca ggtgagcaac
     6781 ggtcaatcct gagaaagcga cggcaatcat gaaaaggaag atcgcggcga aggaaggaga
     6841 ggagggtaac tctccgtctc aactggtcga tgcaaaaatt aagggagtca ccgactggcg
     6901 cgacgcgatg ctcgcgcgca tccggcagct catcaaagag gccgatcccg aggtggtcga
     6961 ggaggtgaaa tggcggaagc cgtcgaaccc ggccggcgtc ccggtgtggt cgcacgacgg
     7021 gatcatctgc accggcgaga cgtataagga caaagtgaag ctgaccttcg ccaagggtgc
     7081 ctcgctcgag gaccctacgg gcctcttcaa cgccagcctc gacggcaacg tgagacgtgc
     7141 catcgatctc catgagggcg acaaaatcga tgaggacgct ttcaaagcac tcatccatgc
     7201 cgccgtcgcg ctgaacacac gcgccgctcg accccgctct cagaagcggg caaagagcgc
     7261 ttgaggatga agtgacacgc ttagcagcac gcagccgctc tacctttctc tccgggagag
     7321 gtcggcccgc agggccgggt gagggatgag cagcagcaat cgcccgaacc ccctctcccc
     7381 gccgggagag ggagaaacgc gaagttcatt caatcgagaa acgtccgccg ccgcagcacc
     7441 agcggcccgt cgctcagctc gtcgttctgt tcggagaggt gagctgcggc gcgatttccg
     7501 aggggtcttt caccttgcgg gaacggccgt cgaagctggc gccctggtcg atcgcgagcg
     7561 cctggctgtg gatgtcgccc tcgaccacgg cgctctcgtg cagcacgacg cgcgagccca
     7621 ggatcgcccc ctgcaccttg ccgtagacgt ccaccgtctc ggccgcgatg gtgccgttga
     7681 tgacggcctc ttcgcccacc gtcagcttca tgctgtgcag gtcggcctgg atgttgccgt
     7741 tgacgcgcag cgagccctgg cagcggatgg tgatcgcctg cccctcgatg gtgaggtcgt
     7801 tgccgatcac ggattcgtcg gcatcctggc ccgacgtgcc ggaaaagcca ttgctgcgca
     7861 tcggagtggg tgtcggagtg cctgttgcct gatggtccat ggtcttgatc tgcagcgggt
     7921 cgacgttgcc ggacctcggt tcgggcttgc gcgtgaacac cattactctg aactcccctc
     7981 ggactaacaa ttccggggtc gaacatctat cgtggtgatg gtcgaattgt gtcattcgac
     8041 cactgatttt ggcgatttgt gacgtaacga agggcgagtt gctgagttgg catacaaccc
     8101 gagtcggctc tagccatcac ccgcgcgggg tcgccacccc tatccgctac tggccattcg
     8161 ctattcggtc cccatcaaac ccgatccacg aagctgtcca ccacccgctt gcgccccgcc
     8221 ttgtcgaagt cgaccgtcag cttgttgccg tcgacttccg ccacctcgcc gtaaccaaac
     8281 ttctggtgga acacccgctc gcccacgttg taggacgagg gctgcccggt ggaggaggcg
     8341 acgagctccc cctcgatcac gaccggacca cgcttcggct gatgcccctg ctgccagcgc
     8401 tgctgcgcgc gctgccaacc gggcgtctga taattcgtct ggaagctgcc cacgtcatcg
     8461 aagcggctgc ggccgtaggg gttcgccatc cggtcgaagg ccgaataggc gccgccgaac
     8521 cggctcggcg cctctacgac ctcgacgtgc tgcggcggca gctcgtcgac gaaccgcgac
     8581 ggaacggctg cctggtagag cccgcgcatg cggcggttct gcgcgaacga gacctgcaca
     8641 tgccgcctgg cacgcgtcag gccgacgtag gcgaggcgcc gctcctcctc caggcccgcc
     8701 tggccgttct cgtcgagcga gcgctgatgc ggaaacagcc cttcctccca gccgggcagg
     8761 aagacgacgt cgaactccag gcccttggct gcgtgcaacg tcatcagcga gacgcgatcg
     8821 ccggtgtccg cctgatcggt atccatgacg agcgacacgt gctcgagaaa gccggcgagc
     8881 gagtcgaact cgtgcatgaa gcggatcagc tctttcaggt tctccagccg cgattgggcc
     8941 tggggcgact tgtcctgctg ccacatcgcc gtatagccgg actcgtcgag gatcagctcg
     9001 gcgagctccg tgtgcttcat ggagggcacg agcccgcgcc agcgctcgaa cgagttgatc
     9061 agctccatca gcgacttccg ggccttgccg gtgatctcct ccgtctcggt gatggagcgg
     9121 gccgcatggt aaagcggcat gtccgtcgcg cgggcgagcg cgtggatgcg cttcagcgac
     9181 gtgtcaccca gcccgcgttt cggcacgttg atgatgcgct cgaacttgag atcgttggcc
     9241 ggattgagcg tcacctccag gtaggcgatc gcgtccttga tctcctggcg ctcatagaac
     9301 cgcgggccgc caatcacccg atacggcagc ccgagcgtca tgaagcggtc ctcgaaggca
     9361 cgcatctgga acgaggcacg catcaggatg gcgatgtcgt tgagcggctg tccggcttgc
     9421 tgcagcttct cgatttcctc gccgatgatg cgggcttcct cctcgtcgtc ccagaccccg
     9481 gtgacgatca ccttctcgcc gaggccatcg tccgtgtgca gcgtcttgcc aaggcgtccg
     9541 cggttgtggg caatgagctg cgacgcagct ccaagaatgt gtccggtcga gcgatagttg
     9601 cgctccagcc ggatcacttt cgcgccggga aagtccttct cgaaccgcag gatgttttcc
     9661 acctcggccc cgcgccagcc gtagatcgac tggtcgtcgt caccgacgca gcacaggttg
     9721 ggagaagccg agccctgtgc cagcagccgt agccagagat actgcgcgac gttcgtgtcc
     9781 tggtactcgt cgaccagcat gaaccggaag cggcgctggt agtcggccag cacgtccgga
     9841 tgctcgcgca gcagccgcag gctttcgagc agcaggtcgc cgaaatcgac ggcgttgagc
     9901 tccttcagcc gcttctgata gtcggcatag agcgccgcgc ccttgccgtt ggcaaaagca
     9961 aagctctcgc ctgccggcac ctgggccggg ccgaagcccc ggttcttcca gctgtcgatc
    10021 agggcggcga gctgccgggc cggccagcgg tccttgtcga cgccgccggc ctcgatcacc
    10081 tgcttcatca gccggatctg gtcgtcggta tctaggatcg tgaagctgct cttcagcccc
    10141 accagctcgg cgtggtagcg caggatcctg gcgttgatcg aatggaaggt gccgagccaa
    10201 agcatgccct cgacggcccc gccgatgagc tgcccgatcc gctccttcat ctcgcgtgcc
    10261 gccttgttgg tgaaggtgac ggcgaggatc tggctggggc gcgctcgccc ggtggcgagg
    10321 atgtgagcaa ttcgcgtcgt cagcacccgc gtcttgccgg ttcccgcgcc ggccagcacc
    10381 agcacggggc cgtccagggt ctcgaccgcc tgccgctgcg cctcgttcag gcccgcgaga
    10441 tagggtggct ccgccgcccg caacgcccgt tgggagatgg gcagatccgg ctcgagggat
    10501 gccttgcctt ctttccgcgc cgcgggcgca agcgcatcct cgccctgcat ctggtgctta
    10561 cgattcgact gcatgacttg aatatagcac gccgaaagcg gcaattccgg tgcctgcggc
    10621 agccacgcac agcctgttgt acaaccttcg gtgtgtgagg ccggaaagat gtcccatgtt
    10681 cgggcgagct gtgcgataaa tcggggcgga acgtcggagc aacggcagtg ttcacggcgg
    10741 aacacggttc aattggctgt tccgaagtgg atcgtgcccg acggggcttg gatgaggcct
    10801 gatgcggacg ataccaccat tacgcggggt tgattgggca tgaacggcgc cctcctctgg
    10861 atcgccggtc tgatcgtggt gctgctcgcg gcgctgtttg cggtgccgta cgcggtcgat
    10921 tggaacgcct atcgcggcgt tttcgagacg gaggcctcgc gcatgctggg ccgtgaggta
    10981 cgcgtgggcg gcgacgtctc tctccgcctc ttgcctgcgc cttacatcag tttcgaaaag
    11041 ccgcgcatag ccgatgccac ggccggcgag gcgttcttcc gtgccgacag cttcacgatg
    11101 tggctcgcct tgccgccgct gctgcaggga attgtcgagg ccaggcaggt cgagatcgag
    11161 cgaccggtcc tcaggttgca gattcacgag gacggcggcg gcaactggcg cacattcaag
    11221 atcagccggg cggatctgcc cttcgtaccg cgcgggctca ccctgcagtc ggtcaaaatc
    11281 acagatggca taattgctgt tcatggtgtc agcggcgagc cgctggcgaa aatcgagatc
    11341 gccgagggcg agctgtccgc tccagcgctc gaaggccctt accggctgcg cgcggacgtc
    11401 cggtggaatg acgacattcg ggaggtgcgc gcttccacag cgccgcccga tccggacggc
    11461 agcacgcggt tgaagcttgc tgtgcgcgcg cccaagtcgg gcaatgccta taccttcgac
    11521 ggccgcatcg tcgaccttgt cgggcgtccc caattcgatg gcagtttcac cgcgaccatc
    11581 ccggtgacca gcggcacggg cgagctacgg ccgaccacgg tccagtcggc ggatagcgaa
    11641 gcgatcccgc ctgcagctct ccacagcggg gtgttcgagg tccgtgccgc tgtgagcgcg
    11701 aacgccgtaa gcgcgaagct cagcgatttg ggattctcct tcgagcagaa cggccagccg
    11761 caactgctga cgggcactgc ggaagcaagc tggctccaga agcctgtgat ccgggccacc
    11821 ctgacctccc gctggctcga cctcgacagg atcgccgacg ttccttcgaa ttcgaacgca
    11881 ctccaggctc ttcgtagtct agcttccagt gtcaccggct tcctgccgac ccaggccgac
    11941 gtagcggccc ggctcacgat cgaccaggcc acgctcgccg gtgacatggt gagcaacctc
    12001 gtggtggcca tcgagaacgc cggcgccgga ctcaccatca aagagttcca tgccgccctg
    12061 cccggcggct cccgcgtgga cgtgaccggc gcgctgaccg gatccggtac ggcggaagca
    12121 ttcgacggcg agatcctggt gcgcggggcc aatctcaacc gatttctggc ctgggccgcc
    12181 cgcggcaccc agttcgcgga tgcgcccagc gacgccgggt tcgctctcag cggacagttg
    12241 agcctcggcc ctggccggct cgagctcagc aaggcgagca tcgactctgg ccccaaccgt
    12301 ctggaaggag agatcaccta tcggtgggac gggcggcgca atctggtgct ctcgctggag
    12361 accagccgcg ccgacatttc cggagttctg ccgggagtgc tcggccctga tctcctgaag
    12421 gcgaaactcg gcgcactgac tggctcggac ggcggcaatc tcaacagcct cctggcaggc
    12481 ctcgccggca tcgatctgag catgcacatc cgtgccgatg ctctcacgga cggcgcgcgc
    12541 atgctgcaca acgtcgatgc cgacgtgaga ctggaggacg gccggctcag gataccggtc
    12601 tttcgggcct ctaccccgga agggttcaag ctcgagctcg acggcgacgt gcgggatttc
    12661 gctggtgagc cgaagggcgc actgcagggt acgatcgagg cgcccacacg ggcggcattg
    12721 tcggaagctc tcgacctcgc tgctgacgac atcgatggcg aacggcgcca atggctcgcc
    12781 gcgcttgccc cacttcgcct cgctttcgtc agccgctttg gtcagccgcc gggtgaacgc
    12841 gtcgtgatga cgatcgacgg cacatcgcat ggcgagcatc tggtcgctac cctcgtactc
    12901 gaaggaggcc tcgcagagtg gcgtagcaag cctgccgacc tgatgctgac gtccgagagc
    12961 aacgaggtcg cgcgcgtcgt acgcggcctg ctgctcggcg gcaccgaaga acgggaggca
    13021 aggtcgaccc tgccgaaggg ccggctattg gtgaaggctg ccggcgttcc cgagaaggac
    13081 gcagccatct ttgtccagat gattgccggc gacgatctcc agctcctgct gcagggacgc
    13141 ggtgccttgc ccgcgaatgg ctcgcccacc ttcaagggcg agatggaggt cgcggctgag
    13201 aattccggtc gcgcgctcca cttcgccggc attccgctgc ccgccaatgc cgtcgaaggt
    13261 ggacttaaag gccgggcagc cgtaacggtt gccgaccacg cgctgacgtt tgccctcgac
    13321 cggttcgagg tcggcaaggt gcggctcagc ggcgaagcca agcttggtgg caccagcgag
    13381 acgcgcacgc tggatctcga cctgaaggcc gacagtgcct ccctgccccg cctgctggcg
    13441 ctggcgctcg acacggagat ccagacctcg gaaatccccg ctgacatcgc acagctctct
    13501 cagtggcaag accagccgtt cgacttcagt aacctcgagc atgtcaacgg aagcctcaac
    13561 ctcaaggccg acacattggc gctcggacaa ggcttcgagc tcgctgacgc ggtcctcgaa
    13621 gcccagatcg agccgggccg catcacgatc accaagctgg aaggcagggc gctcggcggg
    13681 gccgtctcgg gtgctttcaa actggagaag acggcggcag gcgggcagat gtcgggggct
    13741 ttcgggcttt gggacatccg cctcgaccat atcaagggtg ccagcaatgc gccgatcggt
    13801 gcaggcagcc tgcggctgac attgcaggtc aacggccagg ccgtgacccc gcgcgcactc
    13861 attccggtgc tgacgggcaa gggtgagctg caattcagga acgctcgctg ggagcggctg
    13921 tcggccaagg cgatcgaggc cgcagccgac gccgtgttgt caggcgacac ggcccccacg
    13981 ggtgagccgt tgcgtcaggc gctgcgcaca gcgctcgctc gcgagccgct tttgctgggc
    14041 gacctcaaga tcccggtggt tgttggcgcc ggtgcgctcc gtatagatgg gttcactgtg
    14101 gaaacgccag aagcgcagat catcaaccgg accaccatcg acctcaccga ggtcgccatc
    14161 gacagcgagt ggaagctgca gccgaaggcc acccggggcg gcggcacgcc gcttcccggg
    14221 atatcggtcg tctacgtcgg accgctgaaa tccatcgcga gtctcgagcc gcaactgttc
    14281 atcgacatgc tggagcgcga gctcgccgtg cgcaatatgg agcgggacgt tgagcatctg
    14341 gagcagctgc gtcgcgagga tgaggcccgt gccaaggcgg aggcggagcg gctaaggaag
    14401 ctcgaggagg agcggctgat gcagttccag gagctgcaag gggcgcaggg gctgacgatc
    14461 gacgttccgt tcccatttcc agcctcgccg tccaccgcgc cggcgccgca aagcctcacg
    14521 atcgaacccg agacgccttc acagcatccg caacccgcag ccccgcagca gcgcataacg
    14581 ccccagtggg acggtcagtg gccgccgcca gagcgctctg gcacctcggc cccggcgtct
    14641 tccgctcccg aagtacggcg agccctatag tagcccagaa tccaaacccg gatggcgctc
    14701 gacaaccccg cctcgccgcg gctggcgtcg atctcctcca ccagcgccgc cagcgactgg
    14761 ccacgctccg cggccgcctc tttgagtgcc tcccagaatg gcgcttccag cgatatcgac
    14821 gtgctgtgcc cggcaatggt gaaggagcgc ttcagcggcc gcacggatgc tgactttgct
    14881 ggctgcatca caacccgagg tacgccttcc tgatcatcgg gtgatcctgc aattcggtgg
    14941 aagggccttc gaggacgagc cgcccgtttt ccagcacgta ggtgcgatgc gtcacgttca
    15001 gcacctgctt cacgttctgc tcgacgagga gaatggggac gcccgtttcc gcaacacggc
    15061 ggatggctgc catgacgtgc ttcaccagga tcggcgagag cccgaggctc ggctcatcca
    15121 tgatcagcat cttggggccc agcatcaacg cgcgggcgat ggccaccatc tgctgctgcc
    15181 ccccggaaag ctgaccggcc ggctcatcat aatgagcgag cagatccggg aaaagctggc
    15241 cgacgcggtt caggttttcc tcgcgttgcg ctttggcccg cggcagataa gcgccgagca
    15301 gcaggttgtc gtagaccgtc atattcggga agagctgacg ccccatgggc acgtgcgcaa
    15361 gtcccttctc ggccatttgg tgcggctcga cccgattgat ctcctccccg tcgaaaacga
    15421 tccggccccg ccacggagag atcattccgg aaatcgcgcg cagaattgtc gacttgccgc
    15481 tgccgttgcc gccgaccagc ccgacaagct cgccctgctc gacatgaagc gtgacgccga
    15541 aaacgacctg caggttgccg taacccaagt cgacctcttc gacgctgagg atcggaccgg
    15601 ccatcaatcg gttcccaggt aggcttcgac cacacggctg tcagcgacca cctgctgcgg
    15661 tgcgccttct gcgatcttct caccgttggc gaggacgatg acatgatcgc aaagctcgat
    15721 aatggcctcc atgatgtgct cgaccatgag aatggcgagg cccttctcac gcagcttgcg
    15781 gacaaatgag atcatctcgc gaagcgcagg cggattgagg ccagccatga tttcgtcaag
    15841 gaaaagaatt cgtggctcga tcgcgagagc ccgcgcaagc tcgaggcgtc gcctgcgcgc
    15901 gagattgaga tccatggccg gctgatcggc gcgatccgac aagccgacct cgtccaaaac
    15961 ctcgcgtgcg atacgttccg cggaacggcg tgacgggtgc ttcaggaatg cgggaatcag
    16021 cacgttttcg aggaccgtca gctcctcgaa ggggcggtcg gtctgaaagg tccgtcccat
    16081 cccgtcgcgt gcgcgctgaa agggcgcaag atgcgtcacc tcgcgtccga acatgaagac
    16141 ggcgccttcg tccggccgca cgaagccggt gagcgcattg aacagggtag tcttgcccgc
    16201 gccgttgggc ccgatgagcc ccaggatatc gcgatcggcc agcttcagat cgacatgatc
    16261 gaccgccgtg acgccgccga aacggacggt gaggcgttca gctcttagca cttcgggcat
    16321 accgcctcct gacgagccga cgcgacactt caccgaggat gcctcgcggc aggaagagga
    16381 caatcgcaag gatcagcatt ccgtagatca gcaggttcgc ctgcgcaaat gtcgtgcgga
    16441 agacctcgcc ggaaaggatg agcagcacgg ctcctgcagc aggcccccac accgtcccac
    16501 ggccgccgat gatcgcagtc aaggcgattt gcaccgacaa gagcagatag aagaggacat
    16561 gcggctccag aaacgacagg aatatgccgt agataccgcc gcccactgcc gtcaatgcac
    16621 cggacagcat gaaggccttc aacttgaccg cggtcggatc gatgcccgat gccatggccg
    16681 agttctcgtc ttcgcgcacc gcccgcagtt ggtaaccgaa acgccgcgtc gaaatcagtg
    16741 ccacaatgcc gacggtgatc aaagcgaacg cgagagcgag gtaatattgc gtccagcgag
    16801 agaacaggtc gatgccgaac ggctcaggca gggacgcgat gaacactccg gtcgctccgc
    16861 ccgtcagcca tttctcgttg atggcgatga gccggaaaat ctcggcaaat gcaatggtgg
    16921 acagcgcgaa gtaaggaccc cgcaaaccga acgttatgcg cccccagacg taagcgaggg
    16981 ctgcggccgc cagaatggcc agcattatgg accaccacgg cgccagtccg tacctgcttt
    17041 gcccgagagc cacgaaatag gcgccgaggc cgacgaagaa cgaatggccg aggctcacct
    17101 gcccggccca gcccccgaga atgtcccagg acgtggccca gccgatgaac aacagcatcg
    17161 taatgccgac cgtcaaatac ccgggcagga cgaagggata ggcgagcccg atcaccagga
    17221 gcagggcgag accgaacaaa ttggtattca tacgcgctgc accgagcggc cgaacaatcc
    17281 ttcgggacgg aacaggagga cgagaatgaa cgcgacgagg ccataggcgt cgcgatattc
    17341 cgaagtcaag taggaggcac cgaggacctc caccacgcca agcagcagtc cgccgccgat
    17401 tgctgcgccg atgttgccga gacctccgag cacggtgacc acgaacgcct tcagggtgaa
    17461 tacggtgccc gtcgttgtcg gcgaggcgaa tagtaacgga atcaaagcga cacctgcggc
    17521 caccgccagc atcatgccaa tgccgaaaac gaccgcatga atgcgcttcg tgttgatccc
    17581 gaccaattcc gcgccgagcc tgttctcggc agtcccgcga attgcgcggc caatctcagt
    17641 gcgattgagc aggagataga ggccgccaat ggccgccacc gttgcgatac ccgcaagcaa
    17701 caagacgatc gaaagattca cgcctccacc cagcgaaatg gtcgaggagg catagggaac
    17761 gagcaccgat ttcggctctc cgccgaaaat cagcagcaac gagttcgcaa tgatgagcga
    17821 aagcccgagg gttgccagga gagacgattc ttccggggcg tccacaatcg tcgccaggat
    17881 cacgcgctga atgagaacac cggccaagaa gcccgcgggc agagcgacaa ccagtgaaaa
    17941 ataaggatcc cagccgaggg cctgattgaa gacgatagcg gcatacatcc ccaggacgac
    18001 gaattcgcca tgggcgaaat tgatcgtccg catgacgccg aatatcagcg tcagtccgac
    18061 cccgatcagc gcgtacacgc ccccgatcag caacccgttg gcgatgttct gcagaaaggg
    18121 gatgagatcg gccacgtgga actcgctaaa ggagattggg cgaaccgcat ccaaaacgaa
    18181 ttgcggttcg cgccaagatt gacgtttcag ctatacgctt cgtgagccag gctggtctgc
    18241 cccgcagtca gcgcttgtcc cacggcggcg tgggatggcg cacctctttg gcagcgaggt
    18301 cctcagggaa aaccgtcaca tgctgcccat cctgaatctg cagcaccagg ctccggatgg
    18361 ggttctggtt ctggaacccg tcgaacgact cgaagcggac cgtcgtcacc ggcgtctgga
    18421 ggtccgtctt ggacagggct tcgcgaaccg cctcacgatc ggtcgagccg gcccggcgta
    18481 gtgcgtcggc agcaaccctc aatgcgagat acccctcggc ctcgtagaac gaaggctccg
    18541 atcccgcggc tgccttcatg cgctcataga ggtctttcac accttcgtac ttgacgtcct
    18601 tgctccagct cacgaccgtg gcgacgtact cggacaggtc gccgacctgc tcgatgaagc
    18661 ttggcagcgc cgcggacgtg tcgatcgcga tgacatcggc gctgaggccc acctcccgca
    18721 tctgccgcgt gattgcgatg ccatcatcgg cgtacgacac gatgtagagg atgtcgggct
    18781 gctgcccccg gaaccggttg aggatcggcc ggaaatcggt aacgccctga tcgtaggact
    18841 ggtcaccgat gacctcgtat ccccgctcct tggcaaactt cttgcctgcc tcggcaagcg
    18901 acgtgggcca cgcgccgttg ccataaagca tggcgacggt tttcagctcc gtgtcgccct
    18961 tcttccgcag gaagtcgaaa taatcgaagt aggtccgcgc cacgatggtc gagttgtgct
    19021 tcgcgcggaa aacccacggc gatccgggct tggttatgct gtcatcggcg ctgccgagca
    19081 caaccagcgg aaccttctgg cgcgtcgcca gctgggccat cggtcccgta atggacgagg
    19141 cataagagcc gatgatcagc ggaacaccct ggttgacgaa cgactcgacc gcagccagtg
    19201 cgctctgggc ttccgacctg tcatcctgaa cagtgatctc gagcttctgt ccgttgatgc
    19261 cgccgctgga gttgatctcc tcgagcgcga cttccatgcc cgttttgtag cgctggccga
    19321 atgccgcgaa tgccccggag aggctattga ccactccgac cttcacctgg gcgtgcgcac
    19381 ctgggctggt cattgccacg gctgccgccg cagctgcgat gagcgtaagc ctcttcacgt
    19441 cgcgatcctc cacaattcat tgggctaagt tgcgaggaag atcgctggag gtcatggttg
    19501 tgtcaagcaa agggcaagct ctggcagccg ccgcgctcag cacaaagctg ccaacctgga
    19561 gcgggctcga aaaatgcgcc tcgccggaaa ctaatcggta gtcctcttca gcaaatcgag
    19621 ccgccgctcc tcaaggcggc gctcggcctc cgtgcgctgg cgctcgtg
//
LOCUS       DASVDU010000042        15041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_132764, whole genome shotgun sequence.
ACCESSION   DASVDU010000042 DASVDU010000000
VERSION     DASVDU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15041
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132764"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF226_02995"
     CDS             complement(<1..239)
                     /locus_tag="VF226_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7072982.1"
                     /translation="MTRKRHTARSATEQSTEAPLPEPFARWFQSRGWSIRPHQRALLE
                     KARQRRSTLLIAPTGAGKTLAGFLPSLVELVSNESP"
     gene            379..657
                     /locus_tag="VF226_03000"
     CDS             379..657
                     /locus_tag="VF226_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEX7072983.1"
                     /translation="MERNQFFGGNPVGVLIRLALISIVVGVVMSALDIRPDNILYFLD
                     RTLRRIWEMGFGSIEWLLEHLLLGAIVVIPIWIIARLIGVARRSGRES"
     gene            680..2044
                     /locus_tag="VF226_03005"
     CDS             680..2044
                     /locus_tag="VF226_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7072984.1"
                     /translation="MQLQNPDERQRITARPVGHRDVVAIALPIMLSNATVPLIGFADT
                     VVVGQLGQAHLIGAVAVGATIFNFLYWGFGFLRMGTTGLTAQALGAQDGQEVAANLYR
                     ALLIAVGAGGAMIVAQSLIAWAAFRIMGASAEVEAAARTYFAIRIWAAPAGLVNFALL
                     GWFIGLGRAGLAFAIQIFLNILNVALAVLFVIGLELGIPGVGAAALLAEWSAAILGLA
                     LARADLVRRGATAPLSRVLDPGRMKRTFQVNTDIMIRTLCALTAFVFFMAQSARAGDL
                     VLAANAVLNSISMVAVYLLDGFAFAAESLVGRSIGARVQSRFREAVVVSTIWAGATGA
                     LLSLGIWFAGPLIIEFMTTSAEVRSVSNVYLIWAALMPIVGVWCFQLDGIFIGATRTA
                     DMRNMMILSVVAFFAVWSVLQPLYGNHGLWMSILVFNVVRALTLLARFPALERASFPR
                     SAQV"
     gene            complement(2052..3146)
                     /locus_tag="VF226_03010"
     CDS             complement(2052..3146)
                     /locus_tag="VF226_03010"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEX7072985.1"
                     /translation="MFRTALELARPLLFALDPEQAHEVTLRSMEAGVYPRATGPDDPR
                     LSVDVWGLTLPNPVGIAAGFDKDARVPNAILRMGFGFAEIGTVTPRPQQGNPRPRVFR
                     LIRHRAIINRLGFNNCGHEAALRRLQRRPRFGVVGVNIGANKETADRTADYVLGIEAF
                     YDVASYFTVNISSPNTPGLRDLQAPAALDELLERVMTAREQRVNAGAPRVPIVVKLAP
                     DISEADLPAITERLLAHGVDGIAVSNTTLTRPGLTTTTLAQQAGGLSGRPLFARSTAM
                     LARVYRLTDGRVPLIGIGGIDSPETALAKIEAGASLIQLYTGLVYEGPGLLDKIKEHL
                     VSAVECANASSLADLVGCKAEEWAKRPLEA"
     gene            complement(3146..3520)
                     /locus_tag="VF226_03015"
     CDS             complement(3146..3520)
                     /locus_tag="VF226_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HEX7072986.1"
                     /translation="MVNDRACDAESRIVYKILTRADWEEALRAGRYAGSQDDVRDGFI
                     HLSAASQVAGTAARHFRGIEDLVLVALDAERLGSALKWEPSRGGALFPHYYGPLPADA
                     ALWSKPLPLGADGMPVLPAEVH"
     gene            3663..5465
                     /locus_tag="VF226_03020"
     CDS             3663..5465
                     /locus_tag="VF226_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HEX7072987.1"
                     /translation="MAGRAHLRPNHKYQMFPGIFTAPLRIIELFSDPFRYSVPPYQRR
                     YSWTVKEAGQLLDDILVALGEEEQPVDPDYFLGAILLTDAGGQADWRRGRPRSPQTLA
                     IVDGQQRLITLTILLAVLRDLATEDGTGGSSGLEGLIITEGSSPRSHAYRLQLRGADN
                     DFLVAHVLKPGATSTSPASGEIGPSMEAILEVREHFRAELQALSPAERGRLTGFLLQQ
                     CHAVVIVTNDIDHGHRMFSVLNDRGRPLARKDIIKAEVLGSVSPERTESMLAAWAAAE
                     LRLGDEFDAFFSHLRVIEGKGRLPIIAGIRAVREQAGGSEAFMANVLTPYSEAFELIC
                     RASHHGAPESAEIARRLRYLNWLGSSEWVPSTMKWLSLHSDAPAEMLRFLELIEPFAY
                     ALRLLCIGVGKRTTRFSGVLSAIEDGTLFDPDRSPCMLTRDEERHITYNLRNLHERHP
                     QTCKLVLLRLNDEIVGTPQNFDPADWTVEHVLPQSPGRNGQWRNWFPDNDERELLTQS
                     LGNLVLVRRTQNDKASNQDFNRKKAIYFRHAQSEMPAITREIEAAETWTPAHIRERER
                     RFLDILARIWRLDLSAMQTRDGEVGFLRRRRRAG"
     gene            complement(5502..7115)
                     /locus_tag="VF226_03025"
     CDS             complement(5502..7115)
                     /locus_tag="VF226_03025"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7072988.1"
                     /translation="MIDPAVREAVSQSRAWPFEEARRLIQRLERLKVGPEKAVIFETG
                     YGPSGLPHIGTFGEVARTSMVRHAFHVLTDDQWPTRLICFSDDMDGMRKVPDNVPNRE
                     MLQEHLHKPLTSVPDPFGKFESFAHHNNAMLRDFLDRFGFDYEFFSATACYRSGRFDA
                     TLIKMLEVYDKVTEIILPTLGPERRATYSPFLPICPRTGRVLQVPVLERHPDRGTIVY
                     ADPETGEKVETPVTGGRVKCQWKADWALRWTALGVDYEMAGKDLIDSVTLSSKICRAL
                     GATPPEGFIYELFLDEKGQKISKSIGNGITIEEWLTYAPPESLALFMFQKPKSAKRLY
                     FDVIPRAVDEYLQLLAAYPGQGAKEKLDNAVWHIHAGEPPEAELPISYGLLLNLVAAS
                     NAEDKSVLWGFIRRHAPDATPEKYPMLDQLAGYAVAYYRDFVRPTKKFRLPDATERQA
                     LEALERELGRAAPDSSAEDLQNIVYEVGKANGFADKLRDWFRAIYEVLLGQSQGPRFG
                     SFIELYGIEETRELIRKALAGELVGSAAA"
     gene            7297..7710
                     /locus_tag="VF226_03030"
     CDS             7297..7710
                     /locus_tag="VF226_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEX7072989.1"
                     /translation="MSARLTPQEALTYAMITVSAVDRAMTDDELSRIGSIVQQLPAFQ
                     GYDHDWLVRKAEDCGAVLAEAGGLDKVLDMIAEALPQRLRETAYVLAAEVAASDLKVP
                     IEEVRFLEMLADKLNIDALTCAALERAAKARHQRV"
     gene            7842..9050
                     /locus_tag="VF226_03035"
     CDS             7842..9050
                     /locus_tag="VF226_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7072990.1"
                     /translation="MRSAWIGMLSAALCLGAGAANAQISGDVVKIGVLNDQSGLYADF
                     GGTGSVVAARMAVEDFGGKVLGKPIEVLSADHQNKVDIASNIARQWMDVEGVDAFADL
                     TNSAVALAVQNLTKERGKVALFSGPATTRLTNEDCSPTGFHWTFDTYSQAIGSARAVL
                     QEGGKEWFLLVADYAFGHQMAADLTQEITAGGGKVLGEVRHPTSTNDFSSFLLQAQSS
                     GAQIIGLANAGLDTVNAVKQAGEFGIADGGQKIVGLVLVISDVHAMGLSGAKGLIATT
                     AYYWDRDDASREFAKRYEAKMGSKPGMVQAGVYSSVLHYLKAVEAAGTDDGKAVADKM
                     REMPVDDFFAKGAKIREDGRLIHDMYLIEVKRPEESTGPWDYYKVLRTIPGDQAAQPL
                     AQSKCPLVKK"
     gene            complement(9139..10092)
                     /locus_tag="VF226_03040"
     CDS             complement(9139..10092)
                     /locus_tag="VF226_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEX7072991.1"
                     /translation="MVAIAILDDYQNVALEMADWSRLQANHQITVFNKPFPDEDAVAR
                     ALAGFDVVCLMRERTPFPANLIERLPNLRLIVTSGNRNAAIDIAAAKKRDVLVCGTDS
                     PAYATAELAMGLMLSLARNIHTEFANMRAGGWQTTLGRDLRGSTLGLIGLGRLGGRVA
                     LYARSFEMKVIAWSENLTQQRCDEVGAEKVDKDDLLRRADVISIHTRLSNRTRGLIGA
                     RELRLMKPTAYLINTSRGPIVDEAALIEALQQGRIAGAALDVYDREPLPADHPLRSSP
                     RVLLTPHLGYVTEQTYRVFYGGMVDAIEGWLAGKPVGVIEG"
     gene            10143..10868
                     /locus_tag="VF226_03045"
     CDS             10143..10868
                     /locus_tag="VF226_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEX7072992.1"
                     /translation="MSTLDIALSGGMHLAYLLIILGMIVGLALLPQVWVRRVIDRHSA
                     DRPDFPGTGGEFAQHLVERLGLEGVRVEETKLGDHYDPEAKAVRLLPAHFRGRSLSAV
                     VIAAHEVGHAMQDAAGYPPLKARTRLAKQAQKVESVGAMVMIAAPVLMILSKSPHLLV
                     LQGVVGVLILGLTVLIHASTLPMEYDASFRRALPVLKAGNYIPAKDMRAARQILRAAA
                     FTYVAAAAMSLFNVMRWFRAVRI"
     gene            10865..11422
                     /locus_tag="VF226_03050"
     CDS             10865..11422
                     /locus_tag="VF226_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072993.1"
                     /translation="MSLPLVAFALAVMLGAMATGTDARAQRCATDRDCQPTGLRQEAR
                     CVGDTLVLTERRCLGGTCRSMERHQSCAVRNGGRCIGNVFESTSGRCDALLGRCARHA
                     MRDVCLPSCTCSENILLVSTGQCSPSIGCHRVRTVCKAGCTCEPEPRCLDEPVQKSNP
                     EGAGQREKTVVNPTSGAIRSADLLP"
     gene            11403..12302
                     /locus_tag="VF226_03055"
     CDS             11403..12302
                     /locus_tag="VF226_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7072994.1"
                     /translation="MLIYYPDTPDLPLPPGHRFPSQKYRLLRDLIVRQGILDGWGMAP
                     SPLAKLTELQRAHSPEYVRAVLEGTLDQGIQRRIGLPWSETLARRSRATVGGSLAAAR
                     QALRDGFSGQLAGGTHHAHRDFGSGYCVFNDLAVAALTLLDLEEVARVAVIDLDVHQG
                     DGNAALLTGRTDAFVLSLHGEKNFPFRKVPSDLDIALPDGTDDRSFLRTLAGALDAVA
                     GFRPDLVLYLSGADPLRSDKLGRLDLTFDGLAERDRLVLEFCRRRAIPVSIAIGGGYA
                     DPITDTVEAYANTFRVAREVFRG"
     gene            12437..13771
                     /gene="rimO"
                     /locus_tag="VF226_03060"
     CDS             12437..13771
                     /gene="rimO"
                     /locus_tag="VF226_03060"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7072995.1"
                     /translation="MKVKPPKVGFVSLGCPKALVDSERILTKLRSEGYEIAPDYQGAD
                     VVVVNTCGFLDSAKEESLEAIGEAMAMNGRVIVTGCLGAEKDRIMAAHPGVLAVTGPH
                     QYEEVVEAVHEAVPPLHDPFVDLVPPEGLRLTPRHYAYLKISEGCNNRCSFCIIPSLR
                     GDLVSRPAAAVMYEAERLVQAGVKELLVISQDTSAYGLDLKYAESRWRGAPLRARFFD
                     LTQALGSLGAWVRVHYVYPYPHVDSVIPLMAEGKILPYLDIPFQHASPKVLRAMRRPA
                     AQEKTLERIRRWREICPDLAIRSTFIVGFPGETEEDFTLLLDWLKEARLSRVGCFKYE
                     AVEGAAANDLPGAVPEEVKEERWHRFMAVQQEVSRGLLQEKKGRTIDVLIDEVDEEGA
                     MGRSAWDAPEIDGSVFLNGETGVEPGDIVRATVIEADEYDLWAELAPAPGRA"
     gene            13798..14490
                     /locus_tag="VF226_03065"
     CDS             13798..14490
                     /locus_tag="VF226_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072996.1"
                     /translation="MSSENVVAVPRRGWLTSMKDRKQDTISRLFRIYERTFADELNIR
                     VERNTPSEWFKLLVYVLLSSRRIGKQLSLRATREIIKEGWTTAERMAKTTWEQRVEVL
                     DRAHYVRYDYSTATYLGDTAKLLVERYKGDLRKLREAAERKPDQERRLLKEFKGIGDV
                     GCDIFFREAQAVWSELFPFADKRALRAAEQLGLGKTPQELSKLVPRDQFVHLVGALAR
                     CDLERGYSEVML"
     gene            complement(14526..>15041)
                     /locus_tag="VF226_03070"
     CDS             complement(14526..>15041)
                     /locus_tag="VF226_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7072997.1"
                     /translation="GWAWHLIRRGRAMRSPAIAADGWHLLSDVATSAGVIVGLLLALA
                     TGWQVLDPLLAAAVAVHILWVGWRIVSQSLGGLMDASVAGEMMTRIRKVIAANGDGAL
                     QIHDLRTRMAGRATFIEFHLVVPGSMSVTEAHEICDRLEEALTNEIEGAEVLIHVEPE
                     AEAKAKGALTF"
BASE COUNT         2556 a         4844 c         4907 g         2734 t
ORIGIN      
        1 ggtgattcgt tggacaccag ctcgaccagg ctcggcagga agcccgccag cgtcttgcct
       61 gcgccggtgg gcgcgatgag cagcgtcgag cgccgttggc gcgccttctc cagcagagcg
      121 cgctggtgcg gccggatcga ccacccgcgc gattggaacc agcgggcgaa cggctcgggc
      181 agcggcgctt cggtgctttg ctccgttgcg gatcgggcgg tgtgccgttt tcgggtcatt
      241 ggctcgcggc gttcttgctc ggcggagcgg cgattgctct caggttcagt cgtggttcga
      301 tctatgctag aggctcgggt cgcgcggaga gaagcgcctc tccctgcggc gcggcggctc
      361 agcacaaggc tggagaaaat ggaacgcaat cagttctttg gcggcaatcc cgtcggcgtg
      421 ctcatccgcc tggcgctgat ctccatcgtc gtcggtgtgg tgatgtcggc gctcgatatc
      481 cggccggaca atatccttta ctttctggat cgcacgctac ggcgtatctg ggagatgggc
      541 ttcgggtcga tcgagtggct gctggagcac cttctgctcg gtgcgatcgt cgtaatcccg
      601 atctggatta ttgcgcggct catcggcgtg gcgcggcgct ccggcaggga gtcctgagcg
      661 ggctcatcgg tcctcgaaca tgcagcttca gaacccggac gagcgccagc gcatcacggc
      721 tcggccagtc ggccatcgcg acgtggtggc catcgcgctg ccgatcatgc tgtcgaatgc
      781 gacggtgccg ctcatcggtt ttgccgatac ggtcgtcgtg gggcagctcg gacaggcgca
      841 cctcatcggc gcggtggccg tcggggcgac catcttcaac ttcctctact ggggattcgg
      901 cttcctgcgg atggggacca ccggactgac ggcacaggcc ctcggcgcac aggacggtca
      961 ggaggtcgcc gccaatctct accgggcgct gctgatagca gtcggtgcgg ggggcgcgat
     1021 gatcgtggcc caaagcctga tcgcatgggc tgcgttccgg atcatgggcg catcggctga
     1081 ggtcgaagcc gcggcgcgga cctatttcgc gatccgcatc tgggcggcac ctgcggggct
     1141 cgtcaacttc gcgctgctcg gctggttcat cggcctgggt cgcgccggct tggccttcgc
     1201 catccagatt ttcctcaaca tcctgaacgt ggccctggcc gttctcttcg tcatcgggct
     1261 ggagctcggc attccggggg tcggtgcggc agcgctactg gccgagtggt cggccgcgat
     1321 cctcgggctt gccctggcgc gagcggacct tgtgcggcgg ggcgccactg cgccgctctc
     1381 acgcgtgctc gatccgggcc ggatgaagcg gacctttcag gtcaacaccg acatcatgat
     1441 ccggacgctg tgcgcactga cggcgttcgt tttcttcatg gcgcagagcg cgcgggccgg
     1501 ggacctcgtt ctggcggcca atgccgtcct gaactccatc agcatggtgg cggtctacct
     1561 gctggacggg ttcgcgttcg cggccgaaag cctcgttggc cgttccatcg gcgcacgggt
     1621 gcagtcgcgc ttccgcgagg ccgtggtggt ctcaacgatc tgggccggcg cgacgggtgc
     1681 gctgctttcg ctcggaatct ggttcgccgg tccgctcatc atcgagttca tgacgacgag
     1741 cgccgaggtg cgctccgtgt cgaacgtgta cctcatctgg gcggccctga tgccgatcgt
     1801 cggcgtctgg tgctttcagc tcgacgggat cttcatcgga gcgacccgca ccgccgacat
     1861 gcgcaacatg atgatactga gtgtcgtggc cttcttcgca gtctggtcgg tgctgcagcc
     1921 gctttatggc aaccacgggc tctggatgtc gattctggtc ttcaatgtcg tgcgggcgct
     1981 gacgttgctc gcacgctttc ccgccctgga gcgcgcgtcc ttcccgcgct ctgcgcaagt
     2041 ctgacgcccg gtcaggcttc gagcggcctc ttcgcccatt cttcggcctt gcagccgacg
     2101 aggtccgcga gggaggaagc gttggcgcat tccacggccg agacgaggtg ttccttgatc
     2161 ttgtcgagca gccccggccc ctcgtacacc agccccgtgt agagctggat cagagacgcc
     2221 ccggcctcga tcttggccaa ggccgtttcg ggactgtcga tgccgccgat accgatgagc
     2281 ggtacgcgcc catctgtcag gcggtagacc cgggccagca tcgcggtgga gcgcgcgaac
     2341 aggggccgtc ccgagagacc acctgcctgc tgggccaatg tcgtggtggt gagccccggc
     2401 cgggtgagcg tggtgttcga aacggcgatg ccatcgacgc cgtgggcaag caggcgctcg
     2461 gtgatcgccg gcaggtcagc ctcggagatg tctggcgcga gcttgaccac gatcggtacg
     2521 cggggcgcgc cagcgttgac gcgctgctcg cgtgcggtca tcacgcgctc gagcagctcg
     2581 tccagcgcgg cgggcgcctg aagatcgcgc aagcccggcg tgttcggaga tgaaatgttg
     2641 accgtgaagt agctcgctac gtcgtagaac gcctcgatcc cgaggacgta gtcggccgtg
     2701 cggtcagcgg tttccttgtt ggcgccgatg ttgaccccca caaccccgaa cctcggtcgc
     2761 cgctggagtc gccgcagcgc tgcctcatgt ccgcagttgt tgaagccgag ccggttgatg
     2821 atggcccggt gcctgatgag tcgaaacacg cgcgggcgcg gatttccctg ctgcggccgc
     2881 ggggtgaccg tgccgatttc agcgaatccg aagcccatcc gcaggatggc attgggcacg
     2941 cgagcgtcct tgtcgaagcc cgcagcgatg ccgaccgggt tcgggagtgt caaaccccag
     3001 acgtcgacgg agagccgcgg atcgtcgggg cctgtcgcgc gcggataaac gcccgcctcc
     3061 atcgagcgca aggtgacctc gtgggcctgc tcggggtcga gggcaaatag cagtggccgt
     3121 gcgagctcga gagctgtccg gaacatcagt ggacctccgc cgggaggacc ggcatcccat
     3181 ccgctccaag cggcagtggt ttcgaccaca gggccgcgtc agccggcagc ggaccgtaat
     3241 agtgtggaaa gagggcgcca ccccgggacg gctcccattt cagggccgac ccgagcctct
     3301 cggcatcgag tgccaccaga acaagatctt cgatcccgcg gaaatggcgg gccgccgtgc
     3361 ctgcgacctg actggctgcc gagaggtgaa tgaacccgtc gcgcacatcg tcctgcgagc
     3421 ctgcatagcg tccggcgcgt aacgcctcct cccagtcagc gcgcgtgagg atcttgtaga
     3481 caatgcgtga ttcggcatcg caggcgcgat cgttcaccat cgaactccta ttccagtgtc
     3541 cgcggctgca ggaccgggga ggctcgcagt catggtttgc aaaacttatg aacaagcgcc
     3601 gatcttcgtc cgtggcgact gttcggcgtc ttgcgtgggt gataacctga cacgtggatc
     3661 acatggccgg gcgtgcccac ctccgcccca accataagta ccagatgttc ccaggtatct
     3721 tcactgcgcc ccttcgcatc atcgagctct tctccgaccc cttccgctac tcggttccac
     3781 cctaccaacg ccgctattcc tggacggtca aggaagcggg gcagcttctc gacgacatcc
     3841 tggtcgcgct cggagaggag gagcagccgg tcgacccgga ctacttcctc ggcgcaattc
     3901 tgctgacgga tgccggcggc caggcggact ggcggcgcgg ccgcccccga tctccgcaaa
     3961 ccctcgcgat cgtcgacgga caacagcggc tcatcacgct gaccatcctc cttgccgtcc
     4021 tgcgcgatct tgccacggag gacgggactg ggggctcgtc tggtctcgaa gggctgatca
     4081 ttaccgaagg atcgtcgcct cgaagccacg cttaccggtt gcagctccgg ggggcagaca
     4141 acgattttct cgttgcgcat gtgctgaaac ccggcgccac atccacctcc ccagcctctg
     4201 gcgagatcgg gccgagcatg gaggccatcc tcgaagtccg cgagcatttc cgggccgagc
     4261 tgcaggccct gtcgcctgcc gagcgcggca ggctcaccgg cttcctgctc cagcagtgcc
     4321 atgcggtggt catcgtcacc aacgacatcg accacgggca ccggatgttc tccgtgctca
     4381 atgatcgcgg tcggccgctg gcacgcaagg acatcatcaa ggcggaggtc ctcggcagcg
     4441 tctctccgga gcggaccgaa agcatgctgg ccgcgtgggc ggcggcggaa ctgcgcctcg
     4501 gcgacgagtt cgacgccttc ttcagccatt tgcgcgtgat cgaaggcaag ggccgcctgc
     4561 cgatcatcgc gggcatccgt gccgtgcggg agcaggccgg aggcagcgaa gcgttcatgg
     4621 cgaacgtgct gacgccttac tcggaggcct tcgagctgat ctgccgcgca tcgcaccatg
     4681 gcgcccccga atccgccgag atcgcacggc ggctgcgcta tctgaactgg ctgggcagct
     4741 ctgagtgggt gccgtccacg atgaaatggc tgagcttgca cagtgatgcc cctgccgaaa
     4801 tgctccggtt tctcgagctg atcgagccgt tcgcgtacgc gctgcgcctg ctgtgcatcg
     4861 gagttggcaa gcgcaccacg cggttctcag gcgtcctttc cgccatcgag gacggtactc
     4921 tcttcgaccc ggatcggtcc ccgtgcatgc tgacgcgcga cgaggagcgc catatcacct
     4981 acaacctgcg gaacctgcat gagcgccatc cacagacgtg caagctggtt ctgctgcggc
     5041 tcaacgacga gatcgtcggc acaccgcaga atttcgatcc ggcggattgg acggtggaac
     5101 atgtcctgcc gcagagcccg ggccgcaatg gtcagtggcg taactggttt ccggacaacg
     5161 acgagcgtga gctgctcacg cagtcgctcg gcaatctcgt cctcgtccgc cggacccaga
     5221 acgacaaggc cagcaatcag gatttcaatc gcaagaaggc aatctacttc cggcatgcgc
     5281 aaagcgagat gccggcgatt acgcgcgaga tcgaagcggc cgagacgtgg accccagctc
     5341 atatccgcga gcgtgagcgg cgcttcctcg atatcctggc gcgaatctgg cggctggacc
     5401 tctcggccat gcagacccga gacggcgagg tgggcttcct acgccgcaga cggcgggcgg
     5461 gttgaggcgc acatcatcca gcgcagttgc gccgagtgat gtcacgccgc cgccgatcca
     5521 acaagctccc ctgcaagggc cttccggatc agctctcgcg tttcctcgat cccgtagagc
     5581 tcgatgaacg atccgaaccg cggaccttgc gactggccga gcaggacctc gtaaatcgcg
     5641 cggaaccagt cccgaagttt gtcggcgaag ccgttcgcct tgccgacctc gtagacaatg
     5701 ttctgcaggt cctccgccga cgaatccggt gccgccctgc caagctcccg ttccaaggct
     5761 tcgagcgcct ggcgctccgt tgcgtccggc agccggaact tcttggtcgg gcgcacgaag
     5821 tcgcggtaat aggccacggc gtacccggca agctgatcga gcatggggta cttttcgggc
     5881 gtcgcatccg gcgcatgccg gcgaatgaag ccccacagta cggacttgtc ctcggcattc
     5941 gaagctgcaa cgagattgag cagcaggccg tagctgatgg gcagctcggc ttcgggcggc
     6001 tcgccagcgt gaatatgcca gaccgcattg tcgagcttct ccttcgcgcc ttgccccgga
     6061 taggcggcga gaagctgaag atattcgtcg acggcacgcg ggatcacgtc gaaatagagc
     6121 cgcttcgccg acttcggctt ctggaacatg aatagcgcca gactttcagg gggcgcatag
     6181 gtcagccact cctcgatcgt gatgccgttg ccgatggact ttgagatctt ctgtcccttc
     6241 tcatcgagaa acagctcgta gatgaaaccc tccggcgggg ttgcgccgag tgcgcggcag
     6301 atcttggagg acagcgtcac cgaatcgatc aggtccttgc cggccatctc gtagtcgacg
     6361 ccgagcgctg tccacctgag cgcccagtcc gccttccact ggcacttgac gcggccgcca
     6421 gtcaccggcg tctcgacctt ctcgccggtt tcagggtctg catagacgat cgttcctcta
     6481 tcgggatggc gctcgagcac cgggacctga aggacgcgac ctgtccgcgg acagatcggc
     6541 aggaagggcg aatacgtggc gcggcgttcc ggcccgagtg tcggaaggat gatctcggtc
     6601 accttgtcgt acacttccag catcttgatg agcgtcgcgt cgaaccgccc agaccggtag
     6661 caagccgttg ccgagaagaa ctcgtaatcg aacccgaacc ggtcgaggaa gtcgcgcagc
     6721 atggcgttgt tgtggtgcgc gaagctttcg aactttccga acgggtcggg cacacttgtc
     6781 agcggcttgt gcaaatgctc ctgcagcatc tccctgttcg gcacgttgtc cggcaccttg
     6841 cgcatgccgt ccatgtcgtc cgagaagcag atgaggcgcg tcggccattg gtcatcggtc
     6901 agcacgtgga aggcgtggcg caccatgctc gtgcgcgcca cctcaccgaa agtgccgatg
     6961 tggggaagcc cggaagggcc gtagcccgtc tcgaagatga ccgctttttc cggtccgacc
     7021 ttcagccgct ccagacgttg aatgagccgc cgcgcctcct cgaacggcca ggcacgcgac
     7081 tgactgacag cttctctgac ggcgggatcg atcatattcg tcatcttccg cggaggcagg
     7141 cgtgaatcgc cagcgttttc atttccgtgt tgcggcgcaa cctatgtgcg gcgtcctgaa
     7201 gcgtcaatcg caacggtcgc attctcgcac gacgggcgtg caatacgtgt gatacatgac
     7261 cccatgacct cgcatccgat caaagaagtg gcacacatgt ctgctcgctt gacgccgcaa
     7321 gaagcgctca cctacgccat gatcaccgtt tccgcggtcg atcgcgccat gaccgacgat
     7381 gagctgtcgc ggatcggctc catcgtccag cagttgccgg cgttccaagg ctacgatcac
     7441 gactggctcg tccgaaaggc ggaagactgc ggcgcggtcc tcgccgaggc cggcgggctc
     7501 gacaaggtgc tcgacatgat cgccgaagct ttgccgcagc ggctgcgcga aaccgcttat
     7561 gtgttggctg cagaggtggc cgcttcggac ctcaaggtgc caatcgagga ggtacgcttc
     7621 cttgagatgc tggccgacaa actgaatatc gacgccctca cctgcgcggc gctcgagcgt
     7681 gctgccaagg cgcggcatca gagagtgtga cgaggcgctt ccatcacggt tcgggcaatc
     7741 tgatgcccgc caaccgttcg cacgcttgat agcagcttgc ctcgcttcga ttttcgcgca
     7801 atcgtcacgg gcattgctta caagtgaatg ggagcgaagc gatgcgttcg gcgtggattg
     7861 ggatgctctc ggcagccctg tgcctgggtg caggagcggc aaatgcgcag atctccggcg
     7921 atgtcgtcaa aatcggcgtg ctgaatgacc aatcgggatt gtatgccgat ttcggaggaa
     7981 ccggttctgt cgttgccgcc aggatggcgg tcgaggattt cggcggaaag gtgctcggca
     8041 agccgatcga ggtcctgtcc gccgaccatc agaacaaggt cgacatcgcc tccaatatcg
     8101 ccaggcaatg gatggacgtc gaaggcgtcg atgccttcgc agatctgacg aactcggcag
     8161 tcgcgctcgc cgtccagaac ctgacgaagg agcgcggtaa ggttgcgctg ttttccggcc
     8221 cggcaacgac gcgtctgacc aacgaggact gctcgccgac cggcttccat tggacctttg
     8281 atacttactc ccaggcaata gggagtgccc gcgcggtgct gcaggagggc ggcaaggaat
     8341 ggttcctgct ggtcgccgac tatgccttcg gacaccagat ggccgccgat ctcactcagg
     8401 agatcaccgc tggcggcggc aaggtcttgg gtgaggtgcg gcacccgaca agcaccaacg
     8461 atttctcttc attcctgttg caggcccaga gctccggggc gcagatcatc gggctggcca
     8521 atgccgggtt ggatacggtc aacgcagtga agcaggctgg cgagttcggc atcgcggatg
     8581 gtggccagaa gatcgtcggc ctcgtgctcg tcatcagcga tgtgcacgcc atgggcctta
     8641 gcggcgctaa gggcctgatc gcgaccacgg cctactactg ggaccgcgat gacgcgagcc
     8701 gcgaattcgc caagcggtac gaggcgaaga tgggaagcaa gcccgggatg gttcaggccg
     8761 gcgtttactc gtcggtgctg cattacctga aggccgtgga ggccgcaggc acggatgatg
     8821 gcaaggccgt cgccgacaag atgcgcgaga tgcccgtcga cgatttcttc gcgaagggtg
     8881 ccaagatccg cgaggacggc cggctgatcc acgacatgta cctgatcgag gtgaagcgcc
     8941 ccgaggagtc gacgggtccg tgggactact acaaggtcct gcgcaccatc cccggtgacc
     9001 aggccgccca gccgctggcc cagagcaagt gcccgctggt gaagaagtaa ctctaacctg
     9061 ggacgacttt ccggacggcg cgtgcctcgg caagcgccga tccggtgaga tgaactgccg
     9121 actaccagtt ctcgctttct agccctcgat cacgccgacc ggcttcccgg cgagccaccc
     9181 ttcgatggca tcaaccattc cgccatagaa caccctataa gtctgctcgg tgacgtagcc
     9241 gagatgcggc gtcagcagca cgcgggggct cgagcgcaag ggatggtctg ccggaagcgg
     9301 ctcgcggtcg tacacgtcca gcgcggcgcc tgcgatgcgg ccctgctgca gcgcctcgat
     9361 gagcgccgcc tcatcgacga tcggcccccg tgacgtgttg atgagatagg cagtcggctt
     9421 catcagcctg agctcgcgcg cgccgatcaa cccgcgtgtg cggttcgaga gccgcgtgtg
     9481 aatggagatc acgtccgcgc ggcggagcag gtcatccttg tcgacctttt cggccccgac
     9541 ctcatcacaa cgctgctggg tcaggttctc gctccaggcg atcaccttca tctcgaaaga
     9601 gcgcgcatag agcgcgaccc gcccgcccag ccggccgaga ccgatgagcc caagggtcga
     9661 gccgcgcagg tcacggccca gcgtggtctg ccagccgccc gcgcgcatat tggcgaattc
     9721 ggtgtggatg ttccgggcaa ggctcagcat cagccccatc gcgagctcgg cggtggcgta
     9781 cgccggactg tcggtgccgc agacgaggac gtcccgtttc ttagcggcag cgatgtcgat
     9841 cgccgcgttg cggttgcccg aggtcacgat cagccgcagg ttcggcagcc gttcgatgag
     9901 gttcgccgga aacggcgtcc gttcccgcat gagacagacc acgtcgaagc ctgcgagcgc
     9961 gcgggcgaca gcgtcctcgt cgggaaaagg tttattgaag accgtgatct ggtgattggc
    10021 ctgaagccgc gaccagtctg ccatctccag cgccacgttc tggtagtcgt caagtatcgc
    10081 gatcgcgacc atcttgtagc ctcccgactg tcctgctcta tgctttgcgc ctgagcggcc
    10141 cgatgtctac attggacatc gcgctcagcg gaggcatgca cttggcctat cttctcatca
    10201 ttctcggcat gatcgtcggc ctcgccctgc tgccgcaggt ctgggtgcgc cgcgtgatcg
    10261 acaggcattc ggcagaccgg ccggattttc ccggcaccgg cggcgagttc gcgcagcatc
    10321 tggtcgaacg gctcggcctc gagggcgtgc gcgtggagga aacgaagctc ggcgaccact
    10381 acgatccgga agccaaggcg gtgcgtctgc tgccagccca tttccgcggg cgctccctgt
    10441 cggccgtggt gatcgccgcc cacgaggtcg ggcatgcgat gcaggatgcc gccggctatc
    10501 cgcccctcaa ggcgcgtacg cggctcgcca agcaggcgca gaaggtggag tcggttgggg
    10561 ccatggtgat gatcgccgcg cccgttctga tgatcctgtc gaagtccccg catctcctgg
    10621 tgctgcaggg cgtcgtgggt gtcctcatcc tggggcttac ggtcctcatc cacgcctcaa
    10681 ccctgccgat ggaatatgat gcgagcttcc ggcgcgccct gcccgtgctg aaggctggca
    10741 actacattcc tgccaaggat atgcgggcgg cgcggcagat tttgcgggcg gcagccttca
    10801 cctatgtggc ggcggctgcc atgagcctgt tcaacgtgat gcgctggttc cgggcggtga
    10861 ggatatgagc ttgcccctcg tggcgtttgc ccttgcggtc atgcttgggg cgatggcgac
    10921 cggtacggat gcccgcgcgc agcgctgcgc gacggacagg gattgccagc cgacgggcct
    10981 caggcaggag gcgcgctgcg tcggcgacac attggtcctc acggaacgac gatgcctcgg
    11041 cggaacctgc cgctccatgg agcgccatca gagctgcgcc gtccgcaacg gcggccgttg
    11101 catcggcaat gtgttcgagt cgacgtcagg acggtgcgat gccctgctcg gtcggtgtgc
    11161 gcggcacgcc atgcgcgacg tgtgcctgcc gtcctgcacg tgctccgaga acattctgct
    11221 cgtttcgacg gggcagtgct cgcccagcat cggctgccac cgcgtgcgga cggtctgcaa
    11281 ggccgggtgc acctgcgagc cggagccccg ctgcctcgac gagccggtgc aaaagagcaa
    11341 ccctgaaggg gcagggcaac gagaaaaaac tgtggtgaat ccgacgagcg gggcgatccg
    11401 gagtgctgat ctactaccct gacacgcccg acctgccatt gccgcccggg caccggtttc
    11461 cgtcacaaaa gtaccgcctc ctgcgggact tgatcgtccg gcagggcatt ctggacggct
    11521 ggggcatggc gccgtcgccg ctcgctaaac tgacggagct tcagcgcgcg cattcaccgg
    11581 aatacgtgcg ggccgtgctc gagggtacgt tggaccaggg catccagcgc cgcatcggcc
    11641 tgccgtggag cgaaacgctg gcgcgacgat cgcgggcaac cgtgggtgga tcactcgcgg
    11701 cggccaggca ggcacttcgg gacggctttt ccggacagct cgccggcggc acccatcacg
    11761 cccaccgtga tttcggttcg ggctactgcg tcttcaacga tctggcggtc gccgcactga
    11821 cgctgctcga tctggaggag gtcgcgcgag tcgcggtcat cgatctcgac gtgcatcagg
    11881 gggacggcaa cgccgccctg ctgacgggtc gcacggacgc cttcgtgctg agcctccatg
    11941 gcgagaagaa cttccccttt cgcaaggtgc catccgatct cgacattgcc ttgcccgatg
    12001 gcaccgatga ccgctccttt ctgcgcacgc tggccggtgc tctcgatgcg gtcgccgggt
    12061 ttcggcccga cctggtgctc tatctgtcgg gcgccgatcc cttgagaagc gacaagctcg
    12121 gccgcctcga cctcaccttc gatggactgg ccgaacgcga tcgcctcgtc ctcgagttct
    12181 gccgccgccg cgcgatcccg gtctcgatcg ccatcggagg gggctatgcc gacccgatca
    12241 ccgatacggt tgaagcctac gccaacacgt tccgggtcgc ccgcgaggtc ttccgcggct
    12301 gaccgaccca gcacacgact ggcagttgcg taaggccgcc tcgcgacgtt aaaagccgcc
    12361 gatgctcaag accactgccg ctccaaaatc ccgcaaatcg cgcgccgctg ccgatgcgcc
    12421 tgtccgcgcg cgccgcatga aggtgaagcc accaaaggtc ggcttcgtct cgcttggctg
    12481 cccaaaggcg ctcgtcgatt cggagcggat cctcaccaag ctgcgctcgg aaggctatga
    12541 gatcgcgcct gactatcagg gcgccgatgt cgtcgtcgtg aatacctgcg gattcctgga
    12601 ctcggcgaaa gaggaaagcc tcgaggcgat cggcgaggcc atggcaatga acggccgcgt
    12661 gatcgtcacc ggctgtttgg gggccgagaa ggaccgcatc atggccgcgc atcccggcgt
    12721 gctcgccgtc accggcccgc accagtacga ggaggtcgtc gaggccgtgc acgaagctgt
    12781 gccgccgctc cacgatccct ttgtcgatct ggtgccgccg gaagggctgc ggctcacgcc
    12841 gcgccactac gcgtatctca agatttcaga gggctgcaac aaccgctgct ccttctgcat
    12901 catcccgagc ctcaggggcg atctcgtgag ccggccggca gcggccgtga tgtatgaggc
    12961 ggagcggctg gtgcaggcgg gcgtgaagga gctgctcgtc atcagccagg atacgagcgc
    13021 ctacggtctc gacctcaagt acgcggagag ccgttggcgc ggcgcaccgc tcagggcgcg
    13081 gtttttcgat ctgacacagg cgctcggcag cctcggcgca tgggtgcggg tgcactacgt
    13141 ttatccttat ccccacgtcg acagcgtcat tccgctgatg gcggaaggca agatcctgcc
    13201 ctacctcgac atccccttcc agcacgccag cccaaaggtg ctgcgggcga tgcgccggcc
    13261 agccgcgcag gagaagacac tggagcgcat caggcgctgg cgcgagatat gtccggacct
    13321 ggcgatccgt tccacattca tcgtcggctt ccccggagag acggaggagg acttcacgct
    13381 cctgctcgac tggctgaagg aagcgcggct cagccgcgtc ggatgcttca agtacgaggc
    13441 cgtcgaaggg gccgctgcca acgatctgcc gggcgcggtg cccgaggagg tcaaagagga
    13501 gcgctggcac cgcttcatgg ccgtgcagca ggaggtcagc cgcggcctgc ttcaagagaa
    13561 gaagggccgc acgatcgacg tgctgatcga cgaggtcgac gaggagggcg ccatgggccg
    13621 ctcggcctgg gatgcgcctg agatcgacgg cagcgttttt ctcaatggcg agactggggt
    13681 cgagccgggc gacatcgtcc gtgcaacggt gatcgaggcc gatgaatacg acctctgggc
    13741 cgagctcgcg ccagcgcccg gacgcgcata gtaggaacag cttcccgcac ctcgccgttg
    13801 agcagcgaga acgtcgtggc ggtgccgcgg cgcggctggc tgacttccat gaaagatcgc
    13861 aagcaggata cgatttcgcg actgtttcgc atctacgagc gcaccttcgc cgacgagctc
    13921 aacatccgcg tcgagcggaa tactccgtcg gagtggttca agctgctcgt ctacgtgctg
    13981 ctgtcgagcc ggcgcatcgg caagcagctg tcgctgcggg ccacccggga gatcatcaag
    14041 gagggctgga ccacggccga acgcatggcc aagaccactt gggagcagcg tgtcgaggtc
    14101 ctagatcggg cgcactacgt tcgctacgac tacagcacgg ccacatacct cggcgacacg
    14161 gccaagctat tggtcgagcg ctacaagggc gacctgcgca agcttcgcga ggcagcggag
    14221 cggaaaccgg accaggagcg ccggctcctg aaagagttca aagggatcgg cgacgtcggc
    14281 tgcgacatct tcttcaggga agcccaggct gtctggagcg agctcttccc gttcgccgac
    14341 aaaagggcgt tgcgggctgc ggagcagctc ggccttggca agacaccgca ggagctttcg
    14401 aagctggtcc cccgagacca gttcgtgcat ctcgttggcg ccctcgcccg gtgcgacctg
    14461 gagcgcgggt attcggaggt gatgctttag gcgcgagccg cgaatcgggt cttcacgaat
    14521 tcgccctaga acgtcagtgc ccctttcgct ttcgcttcgg cctccggctc gacatggatc
    14581 agcacctcgg cgccctcgat ttcattggtc agcgcctcct ccagccggtc gcagatctcg
    14641 tgcgcctccg ttaccgacat cgaccccggc accaccaggt ggaactcgat gaaggtggcg
    14701 cggcccgcca ttcgggtacg cagatcatgg atctgcaggg cgccatcgcc gttggcagcg
    14761 atcaccttcc tgatcctggt catcatctcg ccggcaaccg atgcatccat gaggccgccc
    14821 aaggactgtg agacgatgcg ccagcccacc cacaggatgt gcacagcgac cgcggccgca
    14881 agcaatggat cgagaacctg ccaaccggtc gccagcgcca acagcaggcc gacgatcacc
    14941 cccgccgagg tcgcgacgtc cgaaagcagg tgccagccgt cagcagcgat tgccggggag
    15001 cgcattgcgc ggccgcggcg gatgagatgc caagcccacc c
//
LOCUS       DASVDU010000043         8519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_515944, whole genome shotgun sequence.
ACCESSION   DASVDU010000043 DASVDU010000000
VERSION     DASVDU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515944"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VF226_03075"
     CDS             complement(<1..1433)
                     /locus_tag="VF226_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7072998.1"
                     /translation="MLLYAKRSFRASPRRLTGLLRARASAVSAPDLEEARRWLIALGQ
                     IEQAVLDAGLASGGQATAATMAAAMVFLAARSGPNRSASIKQAVGRFDVELQCLYESL
                     GDQELEIRIPEGFAWYALYPDSYAQIAEDWSHRFGGSGADVSIVGLRSIGTALSAVVA
                     QTLRKRGLAVRGCATVRPSGVAPFRYAMLPPGIGPASQNIVVDEGPGLSGSSMIAVAQ
                     AFVDRGADPDSIHFFAGHGYGPGASAGANATAWWNDRRIRTTSPDATHIGGRLLPDAL
                     ASAAEACTGEPADGPAVPLGTAGWQRLAGLRSLPPPVAPIIEAPKKIVRLRSGRRVIL
                     KFAGLALTNRQLWWEGQPGPRRIANRTYVPVEAHCHGWEASPWVEGLRLTGADASIAF
                     VEGHLGPWIAAASTKWLQPGEVQDGISRIAEALSAWAEMRDDRSSAALIEAIAERALA
                     KVGSEPQPCYGDGRLAPHEWIREPDGTV"
     gene            complement(1544..2662)
                     /locus_tag="VF226_03080"
     CDS             complement(1544..2662)
                     /locus_tag="VF226_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7072999.1"
                     /translation="MSQHPRGPRYTSLVEGLPATVPFVGPEAQERARGRSFRARIGAN
                     ESVFGPSPQVVAAMQQAASECWMYADPENHDLKAALARHHGVGFENIVVGEGIDALLG
                     YTVRMLIEPGQPVISSLGAYPTFNFHVAGYGGKLVRVPYAGDREDPEALLETARREDA
                     KLVYFANPDNPMGSWWGAADVQRLIDGLPPGTVLLLDEAYSDTAPADAIPPIDVSNPQ
                     VLRLRTFSKAYGLAGLRVGYGIGEAGLIRSFDKIRNHFGVNRMAQVGALAALADQPYL
                     GRVIGQIAEARERIDAIVRANGLEALPSATNFVAVDCGGDGAFAKRVLEELIARDVFV
                     RMPGVAPLDRCIRISAGTEADLAILSEALPEALRAARA"
     gene            complement(2690..3130)
                     /locus_tag="VF226_03085"
     CDS             complement(2690..3130)
                     /locus_tag="VF226_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX7073000.1"
                     /translation="MTVHMIKLCVGADSIEDLANWQKGRLAAQEAAGQKPRLFHSTRM
                     VPKRQAEILDGGSLYWVIKGVVQVRQRITGFGEGQKPDGTPCCLVFLDPPLIPTHPVP
                     RRAFQGWRYLDPADAPPDLDAQTSQQLAQLPPKMRKELAELGLI"
     gene            3397..4500
                     /locus_tag="VF226_03090"
     CDS             3397..4500
                     /locus_tag="VF226_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX7073001.1"
                     /translation="MTALRELVAENGPMALTLGGLVIGFAFGAIVSKTNFCTMGSISD
                     IVSFGDYRRFRAWVLAAVTALLGAQLLQYAEVVELARSMYLAPSVNWFGNVLGGLMFG
                     YGMVFAGGCPSRNLARVGGGDLRALMALIVIGIFAFMSIGGLLGPIRAWLEQSTAVSL
                     AALNTPTQSLGDVVSASLRLSPTTGALIATTAVAVAGLAYCFKDEGFRSSPVHVLSGI
                     GVGLCVTAGWAVTGLAFDELAKQPVAPISLTFVRPAGDTLEWLERFTALGLPGFGVST
                     VLGAILGSFVTAAATKRFTITTFSDKADTIRVLSGAALMGIGGVLALGCTIGQGVTGV
                     STLAIGSFISFAAIVTGGVVGMKAFERILMAEV"
     gene            complement(4553..5020)
                     /locus_tag="VF226_03095"
     CDS             complement(4553..5020)
                     /locus_tag="VF226_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opioid growth factor receptor-related protein"
                     /protein_id="HEX7073002.1"
                     /translation="MRATEDSSIVAFLSGAGRDARGRSFEDILAFSDREIEVHHDFIQ
                     WLFPLETPSAAVPGSPVLTPEDIDQIRQSDVCRANLVRAAERMARFYEENDHWLVPFD
                     HNHRRITRIIHSLWLLVGQEEAERFLKHIEARVRDSGAKVSETSRRFWRNAIG"
     gene            5242..6783
                     /locus_tag="VF226_03100"
     CDS             5242..6783
                     /locus_tag="VF226_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEX7073003.1"
                     /translation="MGKRVALAGFLHETNTFAPSKATLENFEQGGGYLPICRGEDILT
                     RAPGVNLGISGALAHGAANHWTMVPIVWTGAIPSAHVEKAAFEQIATEIVEGIRAAGR
                     LDGVFLDLHGAMVAEHIDDGEGELIARVRAVVGPEVPIAASLDLHGNVTTRMVEQADV
                     LVAFRTYPHVDMAETGRRTAVQLDALMARGKPFAKAFRRIPFLIPIPWQCTELEPARS
                     IYRQVRDLEAGAVASTSLLMGFPAADFEECAPTVLAYAESQEAADEAADRIHDLMLAN
                     ERAFAGRAYDPDEGVREAMRIAERATRPVVIADTQDNPGAGGDSDTTGMLRALIRNGA
                     KRAAIGIMVDPAAAQAAHRAGEGAEISIALGGKSGIEGDAPFNGTFRVEKLSNGALHA
                     TGPYYGGTHLNLGPSACLSIDGVKIVVASHKAQMADQAMYRFVGIEPRGQAILVNKSS
                     VHFRADFAPIAETILVCTAPGPMPLDPAALPWTKLADGMRLSPGGPVFRKASAGSAAL
                     AEGKE"
     gene            6880..8511
                     /locus_tag="VF226_03105"
     CDS             6880..8511
                     /locus_tag="VF226_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073004.1"
                     /translation="MLKVLGLRRPDIHVARPWAKAATGFLAAALLAGTSLVQPAAAKE
                     KTIVGVMHSGLRVLDPIITTAHITRDHAYMIYDVLIAVDENFTPRPQMADWTISDDKL
                     TYTFTLRDGLKFHDGNPVTAADAVASLKRWGKRDSGGQLIFDVTESLEAKDPKTIVWK
                     LKKPFPALLETVGKQSAVPPFIMPERVANTPPDKAITEYVGSGPFVFVESEYQPGVSV
                     TYKKFADYVPRKEEPSWMAGGKVVKVDQVKWITMPDAQTAINALLSGEIDYIEQVQID
                     LLPLLQASDDVVVETRDDLGYQTMGRMNFKHPPFDNVKIRQAALKALSQKDVLNTLIG
                     NPDYYKVCGAIFGCGTPLADESGAETLIKGGDKEAAKKLLKEAGYDGTPVVLMQPTDV
                     VTLTAQPVVAAQALREVGFNVDMQPMDWQTLVTRRASQAKPSEGGWNLFFTNWMVPEI
                     NSPLISPMLNGRGDQGWFGWPKDDKLEELRAEFIAADTPEKQREVAIKIQKHTLENVI
                     YIPLGQYAAPQARSKKLTGMIPSPVPVFWNVDKAD"
BASE COUNT         1564 a         2803 c         2653 g         1499 t
ORIGIN      
        1 acggtcccgt ccggctcacg gatccattca tggggtgcga gccgtccgtc gccatagcag
       61 ggctgcggct ccgagccgac cttcgcaagc gctcgttcgg caatcgcctc tatgagtgcg
      121 gcggaagacc gatcatcgcg catctcggcc caggccgaga gcgcttctgc tattcgggat
      181 atcccatcct gcacctcgcc aggctgcagc cacttggtcg atgcagctgc gatccacggt
      241 ccgagatgac cctccacgaa cgcgatgctt gcatccgcgc cggtcaatcg aagaccttcc
      301 acccagggcg aagcttccca gccgtggcaa tgcgcctcaa caggaacata tgtgcggttc
      361 gcgatgcgac gtggacccgg ctggccctcc caccaaagct ggcggttggt cagcgccagg
      421 ccggcgaact tcaggatgac acgacgcccc gaccggagtc ggacgatttt cttcggcgcc
      481 tcgatgatcg gggcgacggg cggaggcaaa gatcgaaggc cagcaagccg ctgccaacct
      541 gccgtgccga ggggcaccgc cggcccgtct gcgggctcgc cggtgcacgc ctctgcggcc
      601 gaagccaagg catccggcaa caatcgaccg ccgatatgcg tagcatccgg actggtcgtc
      661 cggatgcggc ggtcgttcca ccaggccgta gcgttggccc cggcagaagc accaggacca
      721 taaccgtggc cggcaaaaaa gtggatcgag tccgggtcgg cgcccctgtc gacaaaagcc
      781 tgggcaaccg cgatcatcga tgaccccgac aggcccggcc cttcatcgac gacgatattc
      841 tgggaagcgg gtccaatgcc cggcggcagc attgcgtatc ggaacggcgc taccccggat
      901 ggccggacgg tcgcgcagcc tctcacagcg aggccccgct tgcggagagt ctgcgccacg
      961 acggcagaga gcgcagtccc aatgctgcgc agtccgacga tactgacatc agcgcctgac
     1021 ccaccaaagc ggtgagacca gtcctcggct atttgtgcgt agctgtcggg atagagagcg
     1081 taccaggcga agccttccgg gattcggatc tcgagctcct gatcgcctaa tgactcgtac
     1141 aggcactgca gctccacgtc gaagcgcccc acggcctgct tgatcgaagc tgagcggttg
     1201 ggaccggacc tcgccgcgag aaacaccatg gctgccgcca tcgtggccgc ggtagcttgc
     1261 cctccgctgg ccagccccgc atcaaggacc gcctgctcga tctgcccgag ggcgatgagc
     1321 catcggcgag cctcctcaag gtcaggcgcg gagaccgctg acgctcgcgc gcgaagcagt
     1381 cctgtcagcc gacgcgggga cgcgcgaaag cttcgtttgg cgtaaagcag caagtccaga
     1441 ggcctcggcg acagttgtca ccgccgcgaa acactggctg caaagcagag ttcctggcca
     1501 gagtggccca cgttgtcgcg aatcgaaccg gctgggtaaa agctcaggcg cgggccgccc
     1561 tgagagcctc gggcagggct tcggacaaga tcgccagatc cgcctccgta cctgcgctga
     1621 tgcgaatgca gcgatcgagc ggtgccacgc ccggcatgcg gacgaagacg tcgcgcgcga
     1681 tcagctcctc cagcacgcgc ttggcgaatg cgccatctcc gccacaatcg accgccacga
     1741 agttcgtcgc cgacggcagc gcctccagtc cgttcgcgcg gacgatcgcg tcgatacgct
     1801 cgcgggcctc tgcgatctgc ccgatgactc gcccgagata gggctggtcg gcaagggccg
     1861 cgagcgcgcc cacttgtgcc atccgattga cgccgaagtg gttgcggatc ttgtcgaaag
     1921 agcggatcag gccggcttca ccgataccat atccgacgcg cagacccgcc aagccgtagg
     1981 ctttcgagaa ggtgcgcaac cggagcacct gcggattgga gacatcgatc ggcggaatgg
     2041 cgtcagcggg ggcggtgtcg gagtaggctt catcgagcaa aaggaccgtg cccggcggca
     2101 gaccatcgat cagccgctgg acatccgccg ccccccacca cgagcccatc ggattatcgg
     2161 gattggcgaa gtagacgagc ttcgcgtcct cccgccgggc cgtttcgagc agggcttccg
     2221 ggtcttcccg gtcacccgcg tacgggacgc gcaccagctt gccgccgtag cccgcgacgt
     2281 gaaagttgaa ggtcgggtag gcaccgagcg acgagatcac cggctgcccc ggctcgatca
     2341 gcatccgcac cgtgtatccg agcaatgcgt cgatgccttc gcccaccacg atgttctcga
     2401 acccgacccc atgatgcctg gccaatgccg ccttaaggtc gtggttttcg ggatcggcat
     2461 acatccagca ctcggaagcg gcctgctgca tggccgcgac cacttgcggc gaggggccga
     2521 agacgctctc gttggccccg atccgcgctc ggaacgatcg cccgcgcgcc cgttcctgcg
     2581 cttccggacc gacgaaaggc acggtcgcgg gcagcccctc gacgaggctg gtgtaacggg
     2641 gtccgcgcgg atgctgtgac acgggcaggt ctccttgcgt atcgaagatt cagatcagac
     2701 ccagctcggc cagctccttg cgcattttcg gcggaagctg ggccaattgc tgtgacgtct
     2761 gggcgtccag atccggcggc gcatcggccg ggtccagata gcgccagccc tggaaggcgc
     2821 gccgcggcac cggatgcgtc gggatgagcg gcgggtcgag aaagactagg cagcacggtg
     2881 tgccatcggg cttctggccc tcgccgaacc cggtgatgcg ctgccggacc tgcaccaccc
     2941 ccttgatgac ccagtagagc gagccgccgt cgagtatttc cgcctggcgc ttcggcacca
     3001 tgcgcgtgct gtgaaacagc cgcggcttct gccccgccgc ctcctgcgcg gcaagccgtc
     3061 ccttctgcca gttggcgaga tcctcgatgc tgtcggcacc gacgcacagc ttgatcatgt
     3121 gcactgtcat cggccgtcgc gttcctccgt gttctccacc acggcacgtg tcattcttgg
     3181 caggctcata caccctgccg gatcgcaatg cgagcgcacc tgaagtgcaa cagcagcgca
     3241 gctgtggcaa acttgaatac cgcaagcgcc gtaccgcctc ggctcgccga tcccccggcc
     3301 cgtggctgac cccgtgcttc agctcgcgct tgctcgccca tgttcaaatc tgcgaatgtc
     3361 cgataagcct cggccgcgca agggagaaag ctgacaatga cggcacttcg cgagctggtc
     3421 gccgagaacg gacccatggc actgacgctc ggcgggctcg tcatcggctt cgctttcgga
     3481 gccatcgtgt ccaagacgaa tttctgcacc atgggctcga tctccgacat cgtctcgttc
     3541 ggagattacc gccggttccg tgcgtgggtg cttgccgccg ttacagccct cctcggtgca
     3601 caactcctgc aatatgccga ggtggtcgag ctggcgcggt ccatgtatct cgcgccgagc
     3661 gtcaactggt tcggcaatgt gctcggcggg ctgatgttcg gctacggtat ggtcttcgcc
     3721 ggcgggtgcc ccagccgcaa tcttgcgcgc gtgggcggcg gcgacctgcg cgcgctgatg
     3781 gcgctgatcg tcatcggtat ctttgcgttc atgtcgatcg gcggcctttt ggggcccata
     3841 cgcgcatggc tcgagcaatc gaccgccgtc agccttgccg cattgaatac gccgacccag
     3901 agcctcgggg atgtggtgag cgccagcctt cgcctcagcc ccacgacggg tgcgctgatc
     3961 gcgacgacgg cagtggccgt tgccggcctc gcctactgtt tcaaggacga gggcttccgc
     4021 agctcaccgg ttcacgtatt gtccggcatt ggcgtcggcc tctgcgtcac ggccggatgg
     4081 gcggtgacgg gcctcgcgtt cgacgagttg gccaaacagc cggttgcgcc gatatcgctc
     4141 accttcgtcc ggcctgcggg cgatacgctg gaatggctcg agcgtttcac ggctctcggg
     4201 cttcccggtt tcggggtctc gaccgtcctc ggcgccattc tcggctcctt cgtgacagcc
     4261 gccgccacga agcggttcac gatcaccacg ttttcggaca aggccgatac gatccgtgtg
     4321 ctctcgggcg cagcgctgat gggcatcggg ggcgtactcg cactgggctg caccattggc
     4381 caaggcgtta ccggcgtgtc gacgctcgcc atcggctcgt tcatcagctt cgccgcgatc
     4441 gtcacgggtg gcgtcgtcgg aatgaaagcc ttcgagcgca ttctgatggc tgaggtctga
     4501 tatcctccgt catcccgacg gcgcacgaga gcagcttgag acttatccct tttcacccga
     4561 tcgcattgcg ccaaaagcgt cgactggtct cggagacctt cgcgccgcta tccctcacgc
     4621 gcgcctcgat gtgcttgagg aacctttcgg cctcctcctg ccctacgagc agccaaagcg
     4681 agtggatgat ccgggtaatc cgcctgtggt tgtggtcgaa cggaacgagc cagtggtcgt
     4741 tctcctcata aaacctcgcc atgcgttcgg ccgcgcgaac gaggtttgcc cgacacacat
     4801 cagactggcg gatttggtcg atgtcctcgg gtgtcaggac gggcgagccc ggtacggcgg
     4861 cactcggcgt ctccaatggg aagagccact ggatgaagtc atgatggacc tcgatctcgc
     4921 gatcgctgaa agccagaata tcctcgaagg aacggccgcg agcatcccgc ccagcgcccg
     4981 agagaaaagc tacgatgctt gaatcctcag tggccctcat cgcttcgcct cccgcacccc
     5041 atcggcagaa tcccttgaca cccgactcga accaaaacta acctcgctca acaccttaat
     5101 gcctcacgta aggcattggc gggcacggca ataacttcgg gcgggagtgt acggctgagt
     5161 tggcaaatcg gacggtttcc tcggctttct cctccgaaca actcaacgcg catcatcccg
     5221 gtttcagttc ggggaggcct aatgggcaag cgcgttgcgc tggctggatt cctgcacgaa
     5281 acgaatacct ttgcgccatc gaaagcaacg ctcgaaaatt tcgagcaagg tggcggatat
     5341 ctgccgatct gtcgcggtga ggacattctc acgcgagctc cgggtgtcaa tctcgggatt
     5401 tctggtgcac tggcccatgg cgctgcaaat cactggacga tggttccgat cgtctggacc
     5461 ggcgcaattc cctccgctca cgtcgagaaa gccgcattcg agcaaatcgc gaccgagatc
     5521 gtagaaggca tcagggctgc gggccggctt gacggcgtct ttctcgatct gcatggcgcg
     5581 atggtcgcag agcatatcga tgacggcgaa ggcgagctga tcgcccgtgt gcgcgcagtc
     5641 gtcggccccg aggttccgat cgccgccagc ctcgacctcc acggaaatgt gacgacgcgc
     5701 atggtggagc aggcggatgt ccttgtcgcc ttccgcacgt atccacacgt cgatatggct
     5761 gaaaccggcc ggcgcacggc cgttcagctc gatgcactca tggcgcgcgg aaagccattc
     5821 gcaaaggctt ttcgccgtat ccccttcctt attcccattc catggcagtg caccgaattg
     5881 gagcccgcga gaagtattta tcggcaggtg cgcgacctag aagccggagc agttgcgagc
     5941 acgtcccttc tcatgggctt cccggcggcc gatttcgagg aatgcgcgcc gaccgtgctc
     6001 gcttacgcgg aatcccaaga agccgcggac gaagccgcag atcgcatcca cgacctcatg
     6061 cttgcgaatg agcgcgcctt cgctggccgc gcttatgacc cggacgaggg cgtgcgcgag
     6121 gccatgcgga tcgccgagcg cgcgacccgg ccggttgtca tcgcggatac ccaggacaac
     6181 cccggcgcgg gtggcgactc cgacacgacc ggcatgctgc gcgcgctcat acgtaatggc
     6241 gccaaacggg cggccatcgg catcatggtc gatccggcgg cagcgcaggc ggcacaccgg
     6301 gccggcgagg gagcggagat ctcgatcgct ctcggcggca aatcgggaat tgaaggcgac
     6361 gcgcctttca acgggacctt ccgggtcgag aagctctcaa acggcgctct ccatgccacc
     6421 ggaccctatt acggcggcac gcatctgaac ctcggcccgt ctgcctgcct ctcgatcgac
     6481 ggcgtgaaga tcgtggtcgc cagccataag gcccagatgg ccgatcaggc gatgtaccgg
     6541 ttcgtgggga tcgagccgcg cggtcaggcc attctcgtca acaagagctc cgtccacttc
     6601 cgcgccgatt tcgcgcccat tgcagaaacc attctggtct gcacggctcc cggccccatg
     6661 ccgctcgacc cggctgcgct gccctggacg aagctggccg acggcatgcg cctctcgccg
     6721 ggaggacccg tgttccgcaa agcttccgcg ggatctgcag ccctggccga ggggaaagaa
     6781 tagggcgcga gtctcgtaga caatgcggcg aaattgcccg acactttgct catccttgcg
     6841 tgacttcaac ccaacctgca accgataggg ggatgcacga tgttgaaagt cctcggcctg
     6901 cgtcggccag acatccatgt agcccgtccg tgggccaagg cggcgaccgg cttcctcgca
     6961 gccgccctgc tggcgggcac cagcctggtt cagcctgcag ccgcaaagga aaagaccatt
     7021 gtcggcgtta tgcattccgg actgcgcgtg ctcgatccga tcatcaccac cgcgcacatc
     7081 acgcgcgacc acgcctacat gatctacgac gtgctgattg cggtcgatga gaacttcacg
     7141 ccgagaccgc agatggcgga ctggacgatt tccgatgaca agctgaccta cacgttcacg
     7201 ttgcgcgacg ggctgaagtt ccacgacggt aatcccgtca cggcagccga cgcagtcgcc
     7261 tcgttgaagc gttggggcaa gcgcgactcg ggcggccagc tcatcttcga cgtgactgaa
     7321 agcctcgaag cgaaggatcc caagacgatt gtctggaagc tcaagaagcc cttcccggcc
     7381 cttctcgaga ccgtcggcaa gcagtcggcc gttccgccct tcatcatgcc tgagcgcgtc
     7441 gccaacacgc cgcctgacaa agcgataacg gagtatgtcg gctccggtcc gttcgtcttc
     7501 gtggagtccg aatatcagcc cggcgtcagc gtcacttaca agaagtttgc cgattacgtg
     7561 ccacggaagg aagagccgag ctggatggcc ggcggcaagg tggtcaaggt cgaccaggtg
     7621 aagtggatca cgatgccgga cgcccagacg gcgatcaacg cgctgctgtc cggcgagatc
     7681 gactacatcg agcaggtgca gatcgacctg ttgccgctgc tgcaggcgag cgatgacgtc
     7741 gtcgtcgaga cgcgcgacga cctcggctac cagaccatgg ggcgcatgaa cttcaagcac
     7801 ccgcccttcg acaatgtgaa gatccgccag gcggcgctga aggcgctgag ccagaaggat
     7861 gtgctcaaca ccctgatcgg caaccctgac tattataagg tttgcggcgc catcttcggc
     7921 tgcggcacgc cgctggccga cgagagcggc gccgagacac tcatcaaggg cggcgacaag
     7981 gaagcggcca agaagctgct caaggaggcc ggctacgacg gcacgccggt cgtcctgatg
     8041 cagccgacgg acgtcgtcac ccttacggct cagcctgtcg ttgctgcaca ggcgctgcgt
     8101 gaggttggct tcaacgtcga tatgcagccg atggactggc agacgctggt gacgcgccgc
     8161 gccagccagg ctaagccctc cgaaggcggc tggaacctgt tcttcacgaa ctggatggtg
     8221 ccagagatca actcgcccct catcagcccc atgttgaacg gccgcggcga ccaaggctgg
     8281 ttcggctggc cgaaggacga caagcttgag gagctgcgtg cggagttcat cgccgcggac
     8341 acgcctgaga agcagcgcga ggtggccatt aaaattcaga agcacacgct cgagaacgtg
     8401 atttacatcc cgctcggcca atatgctgcc ccgcaggcac ggagcaagaa attgacgggc
     8461 atgatccctt caccggtgcc cgttttctgg aacgtcgaca aggccgactg acccgcctg
//
LOCUS       DASVDU010000044        12538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1321175, whole genome shotgun sequence.
ACCESSION   DASVDU010000044 DASVDU010000000
VERSION     DASVDU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12538
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321175"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1187)
                     /locus_tag="VF226_03110"
     CDS             complement(261..1187)
                     /locus_tag="VF226_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX7073005.1"
                     /translation="MLEVRSIIRTTGTLIASIGLAAALLGPGASRAQEPLPWEKPAPS
                     SAGAYAAREDAYRPSSGYGGSADYPQRGAYRADDAYRPDDAYRPGSGGDAYVPPPPGS
                     SAGYEPYSEPRPAPYGRDYGPPPEPHNGYGDPPPPPDYGEYDRYDDRTYSRNEILDTG
                     HAFFGSVSRGLASVVEYAFQKAGRPNGYILGEDAGGAFIAGLRYGEGTLYTKDAGVHK
                     VYWQGPSVGYDFGGEGSKTMILVYNLRHPYDIYNRFGGVQGSAYFVGGVGIQFQKHGD
                     VVLAPIRSGIGFRLGANVGYLKYTRTPTWNPF"
     gene            complement(1289..2116)
                     /gene="cysQ"
                     /locus_tag="VF226_03115"
     CDS             complement(1289..2116)
                     /gene="cysQ"
                     /locus_tag="VF226_03115"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX7073006.1"
                     /translation="MDVDYKRLANELLGPAVAAGAAIMRHRRAGFAVESKADASPVTA
                     ADREAEEIVLEALGRCAPGIPVVAEEAVSRGECPTLDDAFFLVDPLDGTREFVRGGTD
                     FTVNIGLVVGRVPCFGLIYAPAIDQLYLTLGAGEAAELRISPDTKPEGLRDVRPSPIR
                     TREPDPARLVAVASRSHRTPENDRFVDDPRIVDCRNIGSSLKFCLVARGEADIYPRFG
                     ATCEWDTAAGDAIVRAAGGLVTTLDKAPLLYGKSDNRFVNPGFVVWGRASLAAAMDL"
     gene            2364..3020
                     /locus_tag="VF226_03120"
     CDS             2364..3020
                     /locus_tag="VF226_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEX7073007.1"
                     /translation="MSQRSHPTDLELAALISSKICHDVIGPVGAIYNGLEILDEDDDA
                     EAQSYALEVIRNVTEQASARLQFARFAFGAAGSAGAVIDLSTAEQISRGFIGNGKHKL
                     NWHLPPGQMAKDQVKLLLNMLASAVTALPRGGTIDVAMAGTLAEPAFAIRCRGGGARP
                     PQHLAEFVRGGPLPRLDAMSIQAYYTWRLAGAAGMLLEIHKEEADIILTAQPKVATRR
                     "
     gene            3344..4063
                     /locus_tag="VF226_03125"
     CDS             3344..4063
                     /locus_tag="VF226_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX7073008.1"
                     /translation="MFRMLTLIWAMGIVLYAPQAQAVAPMGEYGPSRTAMVSEVQTSK
                     TAPLANQVKKSAKRTTRARRKTAQRHRVAHARKYTRHHAARSTQATSRRASRTRRSKA
                     VYAYRSRRITEVGSQASVKQQAKRSPRVASSKTRRKHRSVPVGTTMSGLASYYWQPQR
                     VASGGWFNPKAMTAAHKTLPFGTRVRVTNKRNGRSVEVTINDRGPYVGGRIIDLSDAA
                     AGAIGMKSAGVVPVNVEVVGR"
     gene            4182..4673
                     /locus_tag="VF226_03130"
     CDS             4182..4673
                     /locus_tag="VF226_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX7073009.1"
                     /translation="MRSALLAASAVLLLLTGSDHGLAGEPTQASTGGWQTIVLRAETA
                     SAANRGPAVKSVLRTGSIRKHRLEGLASYYRHGEKTASGERFDKRAMTAAHPTLPFNS
                     LVRVTDRKSGNSVVVRINDRGPFVPGRVIDLSEAAAEAIGMTRRGLTPVSLEVLADSH
                     HVK"
     gene            4782..5513
                     /locus_tag="VF226_03135"
     CDS             4782..5513
                     /locus_tag="VF226_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7073010.1"
                     /translation="MLWLLILPLGIAAGTLGGVVGFGSSVLIMPALVAAYGPKEAVPI
                     MALAALMANASRVAVWWREVDWRVNAAYCLTAVPGAALGARALIELDARFLEGMLGLF
                     LIAMIPIRRWLAARGIGIGLGGMAVLGGFIGFLSGLVATTGPINTPFFLAYGLVKGAY
                     ISTEAVGSAAISMTKSIVFQRFGVLPFETIARGLAVGSTLMLGSWLAKRIVLRFDAAQ
                     FRLLLEAMMIVAGIALIWGALVSYS"
     gene            5612..6373
                     /locus_tag="VF226_03140"
     CDS             5612..6373
                     /locus_tag="VF226_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7073011.1"
                     /translation="MAPSEHNPALARIRTAMIEKKITQAELADAADCHPKTIQNLMNG
                     RPVRDQTLFDVCMVLGLDFDEVKALFRGQPMRDEPGQPGLNGEVAPVYMGAYTRAAVE
                     DYIGSYLTLRPGFAGGGTIVAYRTDIAWDAEWPSLLFQERDRPDAPYSHRGRLYVPPA
                     SMFIHLVSLTKGAMRMIVVSQLDRVGQMRGIITTLNKQRAMLVPVSTPIVYRKYHEFS
                     GLRLGEVNPGEAGYADYRQILDETVREGYARFVTI"
     gene            6418..7572
                     /locus_tag="VF226_03145"
     CDS             6418..7572
                     /locus_tag="VF226_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7073012.1"
                     /translation="MTSRTIRVATFDGPGADPVIREVPWPKVPPKAALIQIGACGVCG
                     TDQHILKGHWPKPLPWPFTLGHELAGVIVEIGSELKTDFMGKPIGVGSKLMLPPLMPC
                     GHCDWCLHYPEQANKCLTPVYYGRYLGFEKPPHLWGGWAEYVYVDLEELPGTKIYKLP
                     DDMGLLLGTLSEPLTSCIRAFNRAVKAGGFKWNDTVVIQGTGPIGILAIAAAQEMGAG
                     RVIAVGAPEEPRLKLAREFGAEATVNIEEVTSPEERIRRVRDIVGGYGADLVMDCSGH
                     PTAGPEGIEFLRDGGTYVEMGQFTDAGSIMTSWHRICAKDINILGSWAFTANDLPLGV
                     DMLYKARDKYPWRKMQTLFPFTEEGVKEAVAQAMAMKTVKSTIVPFPQLL"
     gene            7780..9027
                     /locus_tag="VF226_03150"
     CDS             7780..9027
                     /locus_tag="VF226_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7073013.1"
                     /translation="MYRVRTDAADTITQWSVLLGTVLLACAALLIVLPSTSSANSAKP
                     NIASQLAPTRFVVLLDRPVDFEVLSLTNPNRVVISLPDVKMRLPAGGEDAGAGLVKSF
                     RGGVSAPGQARVVIDVRAPVIVRRSEIQKHGRDHQLVVDLVPAPFGKLPAQAAAKRPL
                     MVQPPLPRPASRPEEIVARSYKPVIVIDPGHGGHDTGAKKNGVVEKDVVLAFSKVLRD
                     KLESTGRYRVLMTRDKDVFITLDGRREIAERNKAALFIAVHADYAGAHARGATIYSLR
                     DSTAAALQRSAKSEVVKTVLTNQELSGVERNDARAIKAILADLAEREVEVTKERTDMF
                     ARSVIEFMSASTPMRPDADREANFRVLKTAKVPSILIELAYVTNPQDARNLQSQVWRD
                     KVSDSIVTAVDNYFSFQIARLPM"
     gene            complement(9850..10767)
                     /gene="rpoH"
                     /locus_tag="VF226_03155"
     CDS             complement(9850..10767)
                     /gene="rpoH"
                     /locus_tag="VF226_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX7073014.1"
                     /translation="MVTKSLPTIAGGSVGLARYLEEIRRFPMLEPDEEYMLAKRWQEH
                     ADAEAAQKLVTSHLRLVARIAMGYRGYGLPISEVISEGNVGLMQAVKRFDPDRGFRLA
                     TYAMWWIRASIQEYILRSWSLVKMGTTAAQKKLFFNLRRAKGQLQALEEGDLRPDQVK
                     AIAKRLGVPEEDVISMNRRLAGDASLNAPVRADLDSGEWQDWLVDDSPDQEEELVERD
                     EMEHRRAFLESALTTLNDRERRIFEARRLSDEPATLEDLSTEFGVSRERIRQIEVRAF
                     EKVQKAVQQAAKASEASASAAAAEASASA"
     gene            complement(11010..12047)
                     /locus_tag="VF226_03160"
     CDS             complement(11010..12047)
                     /locus_tag="VF226_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7073015.1"
                     /translation="MTKRAQHLRLEVPIEATGERLDRFLASRLEDLSRSRIQALIKAG
                     HVRDRRGTIEDPGTRVKPGEMLTVEVPPPEPAEPKPEALPLDVVFEDEHLIVINKPAG
                     LVVHPAAGHASGTLVNALLAHCGSSLSGIGGVMRPGIVHRLDKDTSGLLVIAKTDAAH
                     QGLAKQFAAHGADGRLERAYLAFAWGKFDRPQGTIDAPLGRSVRDRTRMAVVKPGQGR
                     RAVTHFQVIEEYADVEHRTAVSQVKLMLETGRTHQIRVHLAQIGHPLLGDPTYGAHFA
                     ASARRLAPEARAALEKLGRQALHATFLGFEHPITGRMLRFKSPLPADLVALRDALRKA
                     TDQGRLERSRK"
     gene            12084..12464
                     /locus_tag="VF226_03165"
     CDS             12084..12464
                     /locus_tag="VF226_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073016.1"
                     /translation="MPSPSPVDPAAIGEAAPAPDVDARQVRILKLVVIFLGILLFAGF
                     AVVIGRIAYLATHPGRSIGATTNAQDIRLTLPPGAAIRSTAVSGDRMTVQYESPEQTG
                     IVIINLTTGQVVNRITFHPEAPRH"
BASE COUNT         2300 a         4009 c         3870 g         2359 t
ORIGIN      
        1 acgcggcgga tgtgcttgta ggtcaggcgc atggcccgcg cccggtaaat gggcgcaaac
       61 gccagtgcga tgagcgtggc caccaggaag cccagtacca cgagcatggc cgactcgatc
      121 gtgatcactg aggcatcccc ggacgcaatc ctgacagcac agccaagcga ggctgcaacc
      181 ctggtgcagc cccggctacc ttacaacgca gtctcttccc atccgatccc tcgcgcgcgg
      241 cagcagacag gcaaatccgc tcagaaggga ttccacgtcg gagtccgggt atacttcaaa
      301 tacccgacgt tggcacctag gcgaaagcca attcctgaac gaataggtgc aagcaccacg
      361 tcgccgtgct tctggaactg aatgccgacg ccaccgacga aataggcgga gccctgaaca
      421 ccaccaaacc ggttgtagat atcgtacggg tgccggaggt tgtagacgag gatcatcgtt
      481 ttcgatcctt cgccgccgaa atcgtagccg acggacggac cttgccagta caccttatgc
      541 acgccggcgt ccttggtgta gagggttcct tccccgtagc gcagcccggc gatgaaggcg
      601 ccgccagcgt cctcgcccag gatgtagccg ttcggccgcc ccgccttctg gaaagcatat
      661 tcgacgacgc tggccaagcc tcggctcacc gagccgaaga acgcatgacc cgtgtcgagg
      721 atctcgttgc gcgagtaggt gcggtcgtca tagcgatcat actcgccgta atcgggcggt
      781 ggcggcggat caccgtagcc gttgtgcggc tccggtggag ggccatagtc ccgcccgtag
      841 ggcgcgggcc ggggctcgga atagggctca taacccgcac tcgaccccgg cggcggaggg
      901 acgtaagcat cgccgccgct gccaggccgg taggcatcgt cagggcgata ggcatcatcg
      961 gcgcggtagg caccgcgctg cggataatcg gcgcttccgc cgtagccgct actcggccga
     1021 taggcgtcct cgcgcgctgc gtaggcccca gcggacgaag gggcaggctt ctcccaaggc
     1081 agcggctcct gcgcacgact ggcgcccggc ccgagcagcg ccgccgccaa tccgatcgat
     1141 gcgatgagag tgccagtcgt ccggatgatc gagcgcactt caagcacggc tctactcgtc
     1201 attatgctga tgaccgaaaa cggtcgatgg ataattctgc gtcaaccatt ccgcaaaact
     1261 attaccaaag tgtggctgat ctgtcggtct acagatccat cgcggcggcc agcgacgcgc
     1321 gcccccagac gacaaagccg ggattgacga accggttgtc cgacttcccg tagagcaggg
     1381 gggccttgtc gagcgtcgtc accagtccgc cggcggcgcg aacgatcgcg tcgccggccg
     1441 ccgtgtccca ttcgcacgtg gcgccgaatc gcgggtagat gtcagcttcc ccgcgggcaa
     1501 cgaggcagaa cttcagcgat gaaccgatat tgcggcagtc cacgattctg ggatcgtcaa
     1561 cgaagcgatc gttctccggc gtccgatgcg agcggctggc gacggcaacg aggcgggccg
     1621 ggtccggctc gcgcgtgcgg attggagagg gccgcacatc cctgaggcct tcaggcttcg
     1681 tatccggaga gatgcgaagc tctgcggctt ccccggcacc gagcgtcagg taaagctggt
     1741 cgatggcggg cgcgtagatg agcccgaaac atggcacgcg ccccacgaca agtccgatgt
     1801 tcacggtgaa gtctgttccg ccgcggacaa actcgcgcgt accgtcgagc gggtcgacca
     1861 ggaaaaaagc atcatcgagg gttggacatt ccccccggct cactgcctcc tcagccacga
     1921 cgggaatgcc cggcgcgcaa cgtccaaggg cttccagaac aatctcttct gcctcgcgat
     1981 cggcagccgt gaccgggctc gcatcggcct tcgattcgac ggcaaaaccg gcgcggcggt
     2041 ggcgcatgat ggctgcgccg gcggcgacgg ccggcccgag gagctcgttc gcaagtctct
     2101 tgtagtcgac atccataaag tggaacttcc aacctcttga ttctgcgggc tctccagggc
     2161 ctttgcttct gttacgattg tctcaagcag cggcgaacca atccccagga aacttcgatc
     2221 ggaggacagg gcgcggcgat ccgatcttgg caacgatatg gcagcgcatg tatcaacact
     2281 tgaaccgctt ttggcaagga gtcggtccgc gtgagcgcct cccgcgcatg gaccgcccaa
     2341 caatctctgg ttgagggggc ttgatgagtc agcgctcgca tcccaccgat ctcgagcttg
     2401 cagctctcat ctcgagcaaa atctgtcacg atgtcatcgg tcccgtcggg gcgatctaca
     2461 atggcctcga gatcctggac gaggacgacg acgcggaggc tcagtcctat gctctcgagg
     2521 tcatccgtaa cgtgacggag caggcgtcgg cccggcttca attcgcccgg ttcgcgttcg
     2581 gcgcggccgg atcggccggc gcggtcatag acctgtcgac ggctgagcag atttcgcgcg
     2641 ggttcatcgg caacgggaag cacaagctga actggcattt accgccaggt cagatggcca
     2701 aggatcaagt caagctcctg ctcaacatgc ttgcgagcgc cgttactgct ctgccgcgcg
     2761 gcggcactat agatgtcgcg atggctggaa ctctggccga gccggccttt gcgattcgct
     2821 gtcgtggggg cggcgcccgc ccgccgcaac acctggcaga attcgtccga ggcggtcctt
     2881 tgccgaggct cgatgccatg agcattcaag cctactacac atggcggctt gcgggtgcgg
     2941 ccggcatgct gctcgaaatt cacaaggaag aggcggatat cattctgact gcccaaccaa
     3001 aggttgcaac gcgccgctga cactcagaat ccgtaagcag ataatctgaa tgccgtcgca
     3061 tgacggatga acgccgttca atattaagag ttgacggcgg aactaatgag tgctgttttc
     3121 ccggccgcca gatcggaggg aatatccgtt ctccacgggt cctgaatggt ttctggcatt
     3181 ccgctttggc gctatctgaa ttcggtaagc cgaatgtgag ccgacaaagg caatagctca
     3241 cgtcgcgggg gaaacatcct acggccaatc cggccacacc aatccaaaaa ggaggttctt
     3301 ctctgcccgc caaggcaatt gcgaaactgc gtatggaggt ctcatgttca ggatgctcac
     3361 gttgatctgg gcaatgggga tcgtattgta tgccccgcaa gctcaggccg tggcgcccat
     3421 gggcgagtat ggtccgtcga ggaccgcaat ggtttccgag gtgcagacga gcaaaacagc
     3481 tcctctggcc aatcaagtta agaaatctgc aaagaggaca acgcgggccc gccgcaagac
     3541 ggctcaaagg catcgggtgg cgcacgcgcg caaatacacc cggcatcatg cggcacggtc
     3601 gacgcaggcg acgagccggc gcgcttcccg caccaggcgg agcaaggcag tgtacgccta
     3661 tcgctctcgt cgcatcacgg aggttgggag ccaggccagc gtgaagcagc aggccaagcg
     3721 cagccctcgg gtcgccagca gcaagacgcg gcgcaagcac cggtccgtgc cggtaggcac
     3781 gacgatgagc gggcttgcct cgtactattg gcagccgcag cgggttgcat ctggtgggtg
     3841 gttcaatcca aaagcgatga cggctgccca taagacgctg ccgttcggca cccgcgtccg
     3901 ggtcacaaac aagcgtaacg gccgctcggt cgaagtgacc atcaacgacc gcgggccgta
     3961 cgtgggcggt cgcatcatcg acctttcgga tgctgccgca ggcgcgatcg gcatgaagag
     4021 cgccggtgtg gtgcccgtga acgtcgaggt ggtcggcaga taatgccgcc agcgaaaggg
     4081 gagcggcctg gtgtcgtcgc tcctctccac tctgttgatg aagccaactc ccgcccgaat
     4141 cgcccatcac tcctgtgttc atgacgagac gtaggcgcaa gatgagatcg gctctgcttg
     4201 ctgcttcggc agtccttttg ctgctcactg gctccgacca cggccttgct ggcgaaccaa
     4261 cgcaggcctc gaccggcggc tggcaaacaa tcgtgctgcg ggctgaaaca gcttcggccg
     4321 caaatcgcgg cccggccgtg aagtcggtat tgcggaccgg gtccatccgc aagcaccgcc
     4381 tggagggcct cgcctcgtat taccgccacg gcgagaaaac cgcctctggt gagcggttcg
     4441 acaaaagagc tatgacggcg gcccatccga cgctgccctt caactcgctc gtgcgggtga
     4501 ccgatcgcaa gagtggcaac agtgtcgtgg ttcgaatcaa tgatcgcggc ccgtttgttc
     4561 ccggccgcgt catcgatctg tccgaagcag cagcagaagc aatcggcatg acacgccggg
     4621 ggctcactcc cgtcagtctc gaggtcctgg cggattctca tcacgtcaaa tagggttcgt
     4681 acgatctcgc ttcgctggcc gccgtgggcg ttgccatccg gggccccgtc tgccagcttg
     4741 gggcccctga tcgcatcccc attcgatagt gcgccaaccc gatgctgtgg ctgctgattc
     4801 ttccactcgg gatcgctgcc ggcacgctcg gcggcgtggt gggcttcggc tcgtcggtgc
     4861 tgatcatgcc ggcgctcgtc gccgcgtatg gccccaagga ggcggtgccg atcatggcgc
     4921 tcgccgctct gatggccaat gcctcgcgcg tcgcggtctg gtggcgcgag gtcgactggc
     4981 gggtcaatgc ggcctactgc ttaacagccg tccccggagc ggcgctcggc gcgcgcgcgc
     5041 tcatcgagct cgatgcacgc tttctcgaag ggatgctcgg gctttttctc attgccatga
     5101 ttcccatccg gcgctggctt gccgcacgcg gcatcggcat cggccttggc ggaatggccg
     5161 ttcttggagg cttcatcggc tttctcagcg ggcttgttgc cacgaccggc ccgatcaaca
     5221 cacccttctt cctcgcctac ggcctcgtga agggcgcgta catctcgacg gaagcggtcg
     5281 gctcggcggc gatatccatg accaagtcga tcgtgtttca gcggttcggc gtcttgccgt
     5341 tcgaaaccat agcccgcggg cttgcagtcg gctccacgct gatgctggga tcatggctcg
     5401 ccaagcgcat cgtcctgagg ttcgacgctg cgcagtttcg cctgctgctc gaggcaatga
     5461 tgatcgtggc cggcatcgcg ctcatctggg gcgcattggt gtcctactct tgatgtgggc
     5521 gaccgggagc caccactgag tttcaggttt ggttcatccg gaaactgccg ataatttggt
     5581 gcaaattccg gcatctgaag agtcaggcac catggcgcct tctgagcaca atccggctct
     5641 cgcccgtatc cgcacggcca tgatcgagaa gaagatcacc caggccgagc tcgcagatgc
     5701 cgccgattgc catccgaaga ccattcagaa cctgatgaat ggccggcccg tgcgcgacca
     5761 aaccctgttc gacgtctgca tggtgctggg tctcgacttc gatgaggtca aagccctgtt
     5821 ccgcggccag ccgatgcggg acgagccggg ccagccgggc ctcaatggcg aggtcgcgcc
     5881 cgtttacatg ggagcctata cgcgggccgc agtggaggac tacatcggca gctatctgac
     5941 gctgcgtccg ggcttcgcag gtggaggcac catcgtggcc tatcgcacgg atatcgcctg
     6001 ggacgcggaa tggccgagcc tgctgttcca ggaacgcgac agaccggatg cgccctattc
     6061 gcatcgcggc cgcctttacg tcccgcccgc gtcgatgttc attcacctcg tctcgctcac
     6121 caagggcgcg atgcggatga tcgtcgtctc gcagctcgat cgggtcgggc agatgcgggg
     6181 gattattacg acgctgaaca agcagcgggc gatgctcgtt cccgtcagta cacccatcgt
     6241 ctatcgcaag tatcacgagt tttccggcct gcgcctcggt gaggtcaatc ctggcgaggc
     6301 gggctatgcc gattaccggc aaattctgga cgaaacggtc cgcgagggtt atgcccgctt
     6361 cgtaacgatt tgacggctgg gagtaatccg ccgcaccgca tcgggaggaa tgccgccgtg
     6421 acgagcagga caattcgggt agcaacattc gacggccctg gagccgatcc ggtcatccgc
     6481 gaggtgccct ggccgaaggt gccgcccaag gcggccttga tccagatcgg cgcctgtggg
     6541 gtctgcggga cggaccagca tatcctgaaa ggccactggc caaagccgct gccatggccc
     6601 ttcacgctgg ggcacgagct tgccggcgtc atcgtcgaga tcggctccga gctgaagacc
     6661 gacttcatgg gcaaacccat cggcgtcggc tcgaagctga tgctcccgcc gctgatgccg
     6721 tgcggccact gcgattggtg cctgcactat ccggagcaag ccaataaatg cctgacgccg
     6781 gtctactacg gccgttatct gggcttcgaa aagccgccgc atctgtgggg aggctgggcc
     6841 gagtacgtct atgtcgacct cgaggagctg cccggaacga agatctacaa gctgcctgac
     6901 gacatgggcc ttctgctcgg cacactgtct gagccattga cctcgtgtat tcgcgcattc
     6961 aaccgcgcgg tgaaagcggg tgggttcaag tggaatgaca ccgtcgtcat ccagggtacc
     7021 ggcccgatcg gcattctggc catcgcggcc gcccaggaga tgggggcagg gcgcgtcatc
     7081 gcggtcggtg ctccggagga gccgcgcctg aagctcgccc gcgagttcgg agccgaggcg
     7141 accgtcaaca ttgaggaggt gacgtcgccg gaggagcgca tcaggcgtgt gcgtgacatc
     7201 gtcggcggtt atggcgccga tctcgtgatg gactgctcgg gtcacccgac agccgggcca
     7261 gaaggcatcg agttcctccg cgacggcggc acctatgtcg aaatgggtca gttcacggat
     7321 gctggcagca tcatgacgag ctggcaccgc atctgcgcaa aggacatcaa cattctgggc
     7381 tcctgggcct tcacggcgaa cgacctgccg ctcggcgtcg acatgcttta caaggcgcgg
     7441 gacaaatatc cctggcgtaa gatgcagacg ctctttccct tcaccgaaga aggcgtaaag
     7501 gaggctgtcg cgcaggccat ggcgatgaaa acggtcaagt cgaccattgt tccgttcccg
     7561 cagcttctct gaacgacagc ctcattctga accacagatt gtagatcgtc cgtgccaccc
     7621 gtgttgcgca cgagcgcata tatttacctt tcattagggt ttccgctttc ataacattac
     7681 ctccgaggga ccagcaaaaa gccacgattg gactgcatct caagcctgcc cctcgagtgt
     7741 tcagtgcaag gcgtttggcg tttgtggagt tcgagtgcaa tgtacagggt acgtaccgat
     7801 gcggcagaca cgataacgca gtggtctgtc cttcttggta ccgtcctcct tgcctgcgca
     7861 gcgttgttga tcgttctgcc ttcgacctca tcggccaatt ccgcaaagcc caatattgca
     7921 agccagcttg cgcccacacg gttcgtggtg ttgctcgacc gtccggtgga tttcgaggtc
     7981 ctttctctca cgaacccgaa ccgggtcgtc atttcactgc cggacgtcaa aatgcgcctg
     8041 cctgcgggcg gcgaagatgc tggtgcaggc ctggtgaaat cgttccgtgg cggagtgtcc
     8101 gcacccggac aggctcgggt ggtcatcgat gtccgcgcac ccgtcatcgt caggcgatcg
     8161 gaaatccaaa agcacggccg cgatcatcaa ctcgtagtcg atctcgtacc agcacccttt
     8221 ggcaagctgc cggcgcaggc cgcagccaag cgtccgctga tggttcagcc gcccctgccg
     8281 cggccggctt cgcggcccga ggaaatcgtt gcccgctcct acaagcccgt catcgtcatc
     8341 gaccctggcc acggagggca tgacaccggc gccaagaaga acggggtcgt cgaaaaggac
     8401 gtggtgctcg cattcagcaa ggttcttcgg gacaagctgg aaagcaccgg ccgctatcgc
     8461 gtgctgatga cgcgggacaa ggatgtcttc atcaccctcg acgggcgccg cgagatcgcc
     8521 gaacgcaaca aggctgcgct cttcattgca gttcatgcgg attatgccgg cgcccacgcg
     8581 cgcggcgcga caatctactc tctccgggat agcacagcgg cggctctgca gcgttcggcc
     8641 aagtcggaag tggtcaagac cgtgctgacg aaccaagagc tgtccggggt cgaacggaac
     8701 gatgcgcgtg ccatcaaggc gatccttgcc gatcttgcgg agcgcgaggt ggaggtcaca
     8761 aaggaacgga cggacatgtt cgcccgctcc gtcatcgaat tcatgagcgc ctcgaccccg
     8821 atgcgccccg atgcggacag ggaagcgaac ttccgtgtcc tgaaaacggc gaaggtgccc
     8881 tcgatcctga tcgagctggc ctacgtgacg aacccacagg acgcgcgcaa ccttcagtca
     8941 caggtttggc gcgacaaggt cagcgattcc atcgtcaccg ccgttgacaa ctacttctca
     9001 ttccagatcg ctcgcctgcc gatgtgatct gatcgattcg ccgtgggggg aaaggcggcg
     9061 cgaaggccgg cccgcgacac tgggggccgg ccttcatttt tcttgccggt cgacagcttg
     9121 gttcctgtct ccggcccggc ggaactagag gatacttggc ggatgcgaag gtttcagtcg
     9181 ccacagaagc acgtcacacc actcgcccat tcgcgtcccc cctgaaccac tcgaccgctg
     9241 gcctctcttc cagtgctcgc ggccgcgacg caattccaga ccccccgatc tgggaacgga
     9301 cagcggatga aggtgcttca gcatgggctg ctccatcgtc ttgctgtgct gcgccatctc
     9361 ctgccagcgc ggccacccga catgcttcgc gtacttggcc ccccacgggc ctccaacgca
     9421 ggcccgtccg gctttccctc ggcgaagaat gtctgatggc gcagcagaaa ggggacaggg
     9481 aacgcgcgat ctcgcgctgg agtggcgacg ggcgttacgt ccggcggccc tgtccatgtc
     9541 cgccttgcgc ccgccggcag taaggaaggt gccggcttca ttcttgttgt gacgttcaga
     9601 tgccgcaagc tgagttgccg ttgagctctg acgcgcacct ttccacgtga tgggcccgaa
     9661 gatcgagcca ccacggcgag aaaaccgccc gcacgtgcag tctaagtgat tcgcggccga
     9721 tttgttctga aagctgagtt tcgcgatgca actcgcgctg ccagcaaaaa agccggccct
     9781 tgtggaccgg cccttttgct tggctggagt ggagagcagg caaccaatag gttgccgctc
     9841 cagcctccct tacgcgctcg ccgacgcctc ggcggcggca gcacttgcgc tcgcctcgct
     9901 tgctttggcc gcctgctgca cggccttctg caccttctcg aatgcccgca cctcgatctg
     9961 gcgaatccgc tcgcgcgaga cgccgaactc cgtcgacaga tcctcgaggg tggcgggctc
    10021 gtccgagagc cgccgcgcct cgaaaatgcg ccgttcgcgg tcgttgagcg tcgtcagcgc
    10081 cgattccagg aaggccctgc ggtgctccat ctcgtcccgc tcgacgagct cttcctcctg
    10141 gtccggcgag tcgtcgacca gccaatcctg ccattcgcct gagtcgagat cggcgcgcac
    10201 gggcgcgttg agcgacgcgt cgccggcgag ccggcggttc atggagatga cgtcctcttc
    10261 cggcacgccc aaacgcttgg cgatcgcctt cacctggtca ggcctgaggt cgccttcctc
    10321 cagcgcctgc aactggccct tggcccgccg caggttgaag aacagcttct tctgtgcggc
    10381 cgtggtcccc attttcacga ggctccacga gcgcaggata tattcctgga tcgatgcgcg
    10441 aatccaccac atggcatagg tggccagccg gaagccgcgg tccggatcga agcgtttgac
    10501 cgcctgcatc agaccgacgt taccttccga aatgacctcg ctgatcggca ggccgtagcc
    10561 ccgatagccc atggcgatgc gcgccacgag cctgaggtgc gacgtgacca gcttctgggc
    10621 agcttcggca tccgcatgct cttgccaccg cttggcgagc atgtattcct cgtcgggctc
    10681 cagcatgggg aaacgccgga tctcttcgag ataccgcgcg agaccgactg acccgcccgc
    10741 tattgtcggc agcgatttcg tgaccatctc agtttcagcc gagtatcgtg accttgtgtt
    10801 cggcggtgaa acccggttgc tcccttccga cccgtctcat catcactcag tagcgtgacg
    10861 acaccaaggc acaaggacgc atgacacaca acctgcgttt gtgcggtgac gagaacgctt
    10921 ggtgatatcc acggaacgga aaatgacgct tggagttcca ccgcccccta cctacatagg
    10981 actgtttgct tgcctgaaca gtcccgctct catttccttg aacgttcgag cctcccctga
    11041 tccgtcgctt tgcggagcgc atcgcgcaaa gcgacgagat cggccggcag cgggctcttg
    11101 aacctgagca tccttccggt aattggatgc tcgaagccca gaaaggttgc atgcaaggcc
    11161 tgccggccga gcttttccag cgcagctctg gcctccggcg cgagccgcct ggcactggcg
    11221 gcgaagtgcg ctccgtaggt cggatccccg agcagcgggt gaccgatctg ggccaagtgg
    11281 acccttatct ggtgcgttcg gcctgtctcc agcatgagct tgacctgact gaccgcagtc
    11341 ctgtgttcaa cgtcggcgta ttcttcaatg acctggaagt gggtcaccgc gcggcgcccc
    11401 tggcccggct tcaccaccgc catcctggtc cggtctcgca cgctgcggcc gagcggtgca
    11461 tcgatcgtcc cctgcggcct gtcgaacttg ccccaagcga acgccagata ggcgcgctcc
    11521 aggcgcccgt cggccccgtg ggcagcaaat tgcttcgcca ggccctggtg agcggcatcc
    11581 gttttggcga tcacgagcag gccgctcgtg tccttgtcca accgatgcac gatgcctggg
    11641 cgcatgacgc cgccgatgcc ggacagactg gacccgcagt gggcaaggag ggcattgacc
    11701 agcgtgcctg aggcatgtcc agccgcaggg tgcaccacga gcccggccgg cttgttgatc
    11761 acgatcaaat gttcatcttc aaagacgaca tcgagaggca aggcctcggg cttcggctcg
    11821 gcaggctcgg gcggcggcac ctccacggtt agcatctcgc ccggtttgac ccttgtgccg
    11881 gggtcttcta tggtcccgcg ccgatctcgg acgtggcccg cctttatcag agcctggatg
    11941 cgggaccggc tgaggtcttc caaccgcgac gccagaaatc ggtcgagacg ttcgccggtc
    12001 gcctcgatcg gcacctcgag gcgaaggtgc tgggcgcgct tggtcattcg actggcagtg
    12061 cccccgaacg aggattcatg ttgatgccaa gtccctcacc agtagatccc gcagcaatag
    12121 gcgaggccgc gccggcgccc gacgtcgatg ccaggcaggt gcgcatcctc aagctcgtgg
    12181 tcatcttcct tggcatacta ctttttgccg gctttgccgt cgtgatcggg cggatcgcct
    12241 atctggccac ccaccccggc cgcagcattg gcgcgacgac gaacgctcag gatatccggt
    12301 taacgctgcc gcccggggcc gccattcgca gtacagccgt atcgggcgac cgcatgaccg
    12361 tccagtacga gtcaccggag cagacgggca tcgtcatcat caatctgacg accgggcagg
    12421 tggtcaaccg catcaccttc caccctgagg cgccgcgcca ttgacgcagc ttgcctagcc
    12481 aggcccggga cagccaatta gccgcttgat tgcgcagccc cggtcaccta taggttcc
//
LOCUS       DASVDU010000045         6205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1303709, whole genome shotgun sequence.
ACCESSION   DASVDU010000045 DASVDU010000000
VERSION     DASVDU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303709"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF226_03170"
     CDS             <1..195
                     /locus_tag="VF226_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7073017.1"
                     /translation="ALQPAAYSPDLNPIEMAFSKLKSLLKKAAARTVDDLWQAIADAM
                     PRFTPEECANYLAEAGYGAV"
     gene            complement(226..771)
                     /locus_tag="VF226_03175"
     CDS             complement(226..771)
                     /locus_tag="VF226_03175"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073018.1"
                     /translation="MQPATIEHVVPGRIRLKFSGQRGNTEFFKDVVDLVSQFPAVEEA
                     RANPLTGSLLVHYRGDLEDLAQAAVKLGLITVETLSDLLARQRSASGWAAMLRQTPVL
                     LAFALSAIGVLQLARGRALGPASEHFWHARELWLTGKPQAALGLGLLGLLQLSRANLF
                     GSATSLLVYGLMLQNGQQGRR"
     gene            1024..2226
                     /locus_tag="VF226_03180"
     CDS             1024..2226
                     /locus_tag="VF226_03180"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEX7073019.1"
                     /translation="MTATGLLAFMGAAYFSTYAVPEPGYYVLLIRSGAEGGAVGGLAD
                     WFAITALFRRPLGLPIPHTAIVPRSKERIGAAIGRFIEENFLTREVVLRRLRGAQIGH
                     RVVDWLAAPETATDIAGWVTRALPKILHALDNPELQAFVRRTLGEQLERIELAPVLGR
                     LVEAMATTGEADRLFDAATDAGLSWLDAHRDDIYRVVGERNRWWIPKAINRRIATAII
                     DGLTDLLGQLREPDSDARQQFRDALRQLVDELTTSPERRAQIDAARARLLSHPDVQNW
                     LGALWRNALNSALRDLEQPSPKTRASIENLLRAVGRALAADEAAVGQIEAAIERAALT
                     VVVRRAEIGAIVADVVRGWDERSMTDRLEVTIGSDLQYIRMNGTLVGAGVGAMLFVIV
                     HFLALAQL"
     gene            2331..2768
                     /locus_tag="VF226_03185"
     CDS             2331..2768
                     /locus_tag="VF226_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073020.1"
                     /translation="MSSGEPFYFIHSLPRRTRLKVPGRRRDKAFFAEIERLLGALTGV
                     HSVTATPETGSIIVHHAPEFRWSSVRFEAMGMRPADRASKCSCTCPNCSGNGSSELDL
                     ATAAVWMVKAACSGQILLHLIELAITKAVQWAIDDLTKPVVRA"
     gene            complement(2774..5755)
                     /locus_tag="VF226_03190"
     CDS             complement(2774..5755)
                     /locus_tag="VF226_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7073021.1"
                     /translation="MLHQTRSAGPEVGGPGEPVCVRHESPGRLRLGIAGLKNSPELSD
                     LLFVGIGEINGVKNVRPSTLTGNVVILFDPEAISCPTLLRVARALLSGALTPGGAGPG
                     GAAWHTMSVQAVAQGLMTSVQTGLTHRAARDRLAQTGRNVLIPPHVRSSAHILAAQFK
                     SFPVAVLAGATLISVITGALLEAAAISAVVVLNAAIGYVSEARAERILSSLETERSPI
                     ARLLRDGQTREQPAEMVVPGDVIMLQRGDVVPADARVIQAAGLSVSESALTGESLPVS
                     KTVEPLPDPGRALAERTNMVYRGTIVTGGSGAAIVVATGARTEVGRVQQLVARSRPPE
                     TAMQSELDQLGGQLVWITGAASALLVLMGVLRGQNLFLLLRSALTVAVAAIPEGLPMI
                     ATTALSVGVERMRKRGMIVRHINAIETLASVDVVCFDKTGTLTLNQMAVAELASGSRR
                     VAGRELGSSLRRDNLGSDMRRMLEVCCLCSEVELDTNERGELVIEGSATETALVRAAM
                     AAGLDPRQVRARSPLVRIQHRSEAYRFMATLHHVPAGSLLAVKGDPVEVISRCTRVLG
                     PDGRVRYLSPEDREAIQQINREMAGRALRVLGFAYDELVGRPEVIAAEGLTWVGLAGL
                     SDPVRPGVGDVVKRLEKAGVRSIMVTGDAPATARAVAAEIGLTNGRDLRVMTADELKQ
                     LDEAERARIAKMVDVFARVSPAEKLEIVRALQSSGAVVAMLGDGINDSPALRAADVGL
                     AVGRNGDSAAREVADIFLENEDLSLILTGIEQGRATQASVRRALRFLLGTNSSEVMLV
                     LAGTAIFGREVLSPAQLLWINLVTDVLPGIALALEPQAPGLIERKPEQRGAPILGARQ
                     IPRFVGDGALLASSSLIAGLFGSVLYGRSAPQVQRMIFESLVEAQLVHALNCRTQPAT
                     TRALDLVVYGTLALQEIASVLPVTRHLLGLAPVGPLAMGIMAGSAGLSYLAIGRTREP
                     EVSARRSPLHFVRKHEAARMN"
     gene            complement(5821..6126)
                     /locus_tag="VF226_03195"
     CDS             complement(5821..6126)
                     /locus_tag="VF226_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5132 domain-containing protein"
                     /protein_id="HEX7073022.1"
                     /translation="MAIFEDAMKGGNIVTGLAIGVGVLLLSPLVKPLVRPVAKTVLKA
                     GVAAYDQGRAALAELNEQAGDIVAEVRAEMEHERHRAGNGARHAAGEAQPQGGPMPS"
BASE COUNT         1097 a         2102 c         1988 g         1018 t
ORIGIN      
        1 gctttgcaac ctgccgctta ctcgcccgat ctcaatccga tcgagatggc cttctccaag
       61 ctcaagtcct tgctgaagaa ggccgccgca cgcaccgttg atgacctttg gcaggccatc
      121 gcagatgcca tgccgcggtt caccccagag gagtgtgcaa attacctcgc cgaggccgga
      181 tatggcgccg tatgatcgga atctgctcta acccagccgc tccgatcacc gacggccttg
      241 ctgcccgttc tggagcatca gcccgtagac gagcagcgag gttgcagagc caaacaggtt
      301 tgcgcgcgaa agctgaagca gtccgaggag ccccaatccc aaagccgcct ggggctttcc
      361 cgtcagccac agctcgcggg catgccagaa gtgctccgaa gccgggccga gggcccgtcc
      421 gcgcgcgagt tgcagcacgc cgatggcgct cagggcgaag gccagcagaa caggcgtctg
      481 gcgcagcatc gctgcccatc ccgatgcgga acgttgtcgg gccaggagat ccgatagcgt
      541 ttccacagtg atgagcccga gcttgaccgc cgcttgtgca agatcttcca gatctcccct
      601 gtaatgcacc aggagacttc cggtcagcgg attggcccgc gcctcctcga cggccgggaa
      661 ttgactgacg aggtcgacga catccttgaa gaactccgta tttccgcgct gcccggaaaa
      721 cttcagccga atgcggcctg ggacgacgtg ctcgatggtc gcgggctgca taagcgcctc
      781 cggggcggac ttttgggtag ggcaggctcg cctggaacgc gccagcgctc ggccggttcc
      841 ggtttcggca gccctcagga ccccaatcct gccggaacaa accgcgccga ggaagccggc
      901 cagggcaagg tagtggcaat gtccgaaatc gagcatcccc cgcaaaccca ccccgagaca
      961 gaccggcctt ccagtcgcgg cgctcgcgac ggagagcgcc gcgcccggct tcggcggaac
     1021 cgtatgacgg cgacgggcct gcttgcattc atgggtgccg cgtacttctc gacctacgct
     1081 gttccggagc cggggtacta cgtgctgctg atccgttcgg gggcggaggg cggagccgtc
     1141 ggcgggctgg cagactggtt tgcgatcacg gcattgttcc gccgtccgct tggcttgcct
     1201 attccgcaca cggccatcgt gccgcgcagc aaagagcgga tcggcgccgc catcgggcgc
     1261 ttcatcgaag agaactttct gacccgagag gtggtgttgc ggcgcttgcg gggtgcgcag
     1321 atcgggcacc gggtcgtcga ctggctcgcc gccccggaga cggcgaccga catcgccggg
     1381 tgggtgacgc gggcgctgcc caagatcctg cacgcactcg acaatcccga gcttcaagca
     1441 ttcgtccgcc gcacactggg agagcaattg gagcggatcg agctcgcgcc ggttctgggg
     1501 cggctggtcg aggcgatggc gaccacaggt gaagccgacc ggctgttcga tgccgcgacg
     1561 gacgcgggcc tgtcctggct cgacgcacac cgcgatgaca tctaccgcgt cgttggcgaa
     1621 cggaaccgtt ggtggatccc gaaggcgatc aaccgccgca tcgcaacggc aatcatcgac
     1681 ggtttgacag accttctcgg ccagctgcgc gagcccgaca gtgatgcccg tcagcagttc
     1741 cgcgatgccc tccggcagct cgtcgacgag ctcaccacct caccggaacg ccgcgcgcag
     1801 atcgacgccg cccgggcccg gctgctgtct catcccgacg tgcagaattg gcttggcgcg
     1861 ctctggcgga acgcgctcaa cagtgcgttg cgcgacctgg agcagccatc gcccaaaacc
     1921 agagcgtcca tcgaaaacct cctgcgcgcc gtcggaaggg ccctcgccgc cgacgaggcc
     1981 gcggtcggcc agatcgaagc tgccatagaa cgggcggcgc tcacggtcgt cgtgcggcgg
     2041 gcagagatcg gcgccatcgt ggcggacgtc gtgcgcggtt gggacgagcg gtccatgaca
     2101 gatcggctgg aagtcacgat cgggagcgac cttcaataca tccgcatgaa cggcacgctg
     2161 gtcggagcag gtgtcggagc aatgctgttc gtgatcgtcc attttctggc gttggcgcag
     2221 ctatagcgag ggcgcgtcaa cgcaacgcgg cagctgtcac aaaccctaca agaagctctg
     2281 cgagaagcgc ggggcaacaa ggggtgaaac ggcagcaaaa tacgcgccag atgtcttccg
     2341 gggaaccgtt ctacttcatc cacagcctgc cgcggcggac gcgcctgaag gtgcctgggc
     2401 gccggcggga caaagccttc ttcgccgaaa tcgagcggct gctcggcgcg ctgaccggcg
     2461 ttcactccgt gactgcgacg ccggagaccg gcagcattat cgtccaccac gcgccggagt
     2521 tccgctggtc cagtgtgcgg ttcgaagcca tgggaatgag acccgccgat cgcgcttcga
     2581 agtgtagctg tacctgtccg aactgctcag gcaatggctc cagcgagctc gacctcgcga
     2641 ccgccgccgt ttggatggtc aaggccgcct gctccggcca gatccttttg catctcatcg
     2701 agctggccat caccaaagcc gtgcaatggg ccatcgacga cctcaccaag ccggtggtac
     2761 gggcctgaaa acctcagttc attctggccg cttcgtgttt gcgcacgaag tgcagcgggg
     2821 atcttcgcgc ggacacttcg ggctcgcgcg ttctgccgat ggcgagataa gaaagccccg
     2881 ccgatccggc catgatgccc atggcaagcg gcccgacggg agcaaggccg agcaaatgtc
     2941 gggtgacggg cagcaccgaa gcaatctcct gcagggccag ggtcccatag acgaccaagt
     3001 cgagcgccct tgttgttgcg ggttgggtcc ggcagttgag agcgtggacg agctgagcct
     3061 cgacgaggct ttcgaaaatc atcctttgca cctggggggc acttcggccg taaagcacgg
     3121 agccgaataa gcccgcgatg agggatgagc tggccagcaa agcaccgtcg ccgacgaacc
     3181 ggggtatctg gcgcgcgcca aggatggggg cgccgcgctg ttccggcttg cgctcgatga
     3241 ggcccggtgc ctgcggctcg agagcaagcg cgatgccggg cagcacgtcc gtgacgagat
     3301 tgatccacaa gagctgagcc ggactgagaa cttccctgcc gaaaatggca gtcccggcga
     3361 gcaccagcat gacttcgctg ctgttggttc cgagcaggaa tcggagcgcc cggcggacag
     3421 acgcttgggt cgcccggccc tgctcgatgc ccgtcagaat aaggctcaga tcctcgtttt
     3481 cgaggaagat gtcggccact tctctggccg cactgtcgcc gttccggccg actgccagac
     3541 cgacatccgc cgcgcggagt gcggggctgt cgttgatccc gtcgcccagc atagccacga
     3601 ccgccccgga gctctgcagc gcgcgcacga tctccagctt ctcggcgggg ctgacgcggg
     3661 cgaacacatc gaccatcttg gcgatgcggg cgcgttccgc ctcatccagc tgcttaagct
     3721 cgtcagccgt catgacgcga agatcgcggc cgttggtcag cccgatctcc gcggccaccg
     3781 cgcgggcggt cgcgggcgca tccccagtga ccatgatcga gcggacgccc gctttttcga
     3841 gccgcttcac gacgtcgccg acgcccggac ggacaggatc ggaaaggccg gcgagaccga
     3901 cccaggtaag cccttccgcc gcgatcacct cgggccggcc gacgagctcg tcataagcaa
     3961 aacccagcac cctcagcgcc cggcccgcca tctccctgtt gatttgctgg atggcttccc
     4021 ggtcttccgg tgaaaggtag cggacgcgtc catccggtcc gagcactcgt gtacagcggg
     4081 agatgacctc gacaggatct cccttcaccg ccagcagaga gccggccggc acatgatgca
     4141 gtgtcgccat gaaccggtac gcctccgagc ggtgctgtat tcgcaccagc ggcgatcgcg
     4201 cgcggacctg acgcggatcg aggccggccg ccatcgcggc ccgcacgagc gccgtttcgg
     4261 tcgcagaacc ttcgatgacg agctcgcctc gctcattcgt gtccagctcg acctcgctgc
     4321 agaggcaaca aacctccagc attctgcgca tatcactgcc gagattgtcg cgccgcagag
     4381 agctgccgag ctcccggccc gcaacccggc ggcttccgct cgcaagctct gccacggcca
     4441 tctgattgag cgttagtgtc cccgtcttgt cgaaacaaac gacatcgacg gaggccagcg
     4501 tttcgatggc attgatatgg cggacgatca tgccccgctt tcgcatccgc tcgactccaa
     4561 cggaaagcgc agtcgtggca atcatcggca gcccttccgg aatggccgcc acggctacgg
     4621 tcagcgccga gcgcaacagc aggaacaggt tctgccccct gaggacgccc atgaggacca
     4681 gaagcgcgct cgccgcccct gtgatccaga cgagctgtcc gccgagctga tcgagctcgc
     4741 tctgcatcgc cgtttccggc ggccggctcc gcgcaacgag ctgctgcacc cggccgacct
     4801 ccgtgcgggc tcccgttgcc acgacgatcg cggcaccact cccgcccgtc acgatggtcc
     4861 cgcggtagac catgttggtg cgctcggcca gcgcacgccc gggatcgggc agaggctcga
     4921 cggtcttgct caccggaagg ctttccccgg tcagagccga ctcgctcacc gaaaggcctg
     4981 ccgcctggat cacccgggca tcggcgggaa cgacgtctcc acgctgaagc attatgacat
     5041 cgccgggcac gaccatctcg gctggctgct cccgggtctg cccgtcccgc agcagccggg
     5101 cgatgggact gcgctcagtt tcgaggctcg aaaggatgcg ctcggcccgg gcctcgctca
     5161 cgtaccctat tgcggcattc aaaacgacaa cggcagagat cgccgcagcc tccagcaagg
     5221 cgccggtgat gacggagatc aacgtcgcgc cagccagcac ggcgaccggg aagctcttga
     5281 actgggcggc aaggatgtgt gccgaactcc gcacatgggg tgggatcaag acgttccgcc
     5341 cggtttgggc caggcgatcc cgcgcggcgc ggtgcgtcag gcccgtctgg accgacgtca
     5401 tcaacccttg cgccacggcc tgcacgctca tcgtgtgcca ggccgctccg ccagggcctg
     5461 cgcccccggg cgtcagcgcg ccgctcagaa gcgcccgcgc aacccgcagc aatgtcgggc
     5521 aggagatggc ttccggatcg aagaggatga cgacattacc ggtcagcgtg ctcggccgga
     5581 cattcttcac cccgttgatc tcgccgatgc cgacgaacag caaatcggaa agctcgggcg
     5641 agttcttcag gccggcgatt cccagccgaa ggcgaccggg gctctcgtgg cgcacgcaca
     5701 ccggctcgcc ggggccgccg acctctggcc cggcgcttcg cgtctggtgg agcattggct
     5761 ggctcaattc cgccgggagt gatctgctgt ctcggcagcg acccactggt gccgcgctcg
     5821 tcagcttggc atcgggccgc cctggggctg cgcctcgcct gcggcatgtc ttgcgccgtt
     5881 accggcgcga tgccgttcgt gctccatctc cgcccgcacc tccgcaacga tatccccggc
     5941 ctgctcattg agctcggcaa gagctgcccg gccctgatca taggcggcca ccccggcctt
     6001 caggaccgtc ttggcgacag ggcgcacaag cggcttcacc agcggggaaa gcagcagcac
     6061 tccgactccg atcgccagtc cggtaacgat gttgccgccc ttcatggcgt cttcgaagat
     6121 ggccattgcg agcctccagt gggttgagag atttgctcga gagcatgccc cagacgacga
     6181 gcccctgcgt ggcaggaaag taact
//
LOCUS       DASVDU010000046        14488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1674490, whole genome shotgun sequence.
ACCESSION   DASVDU010000046 DASVDU010000000
VERSION     DASVDU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14488
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674490"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VF226_03200"
     CDS             complement(<1..545)
                     /locus_tag="VF226_03200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX7073023.1"
                     /translation="MAFDDFMRAFHEYKATNDRRLAELEAKSADVITEEKLARIDAAL
                     DEQKQQIDYLANKGRLVRPGETVADTHKAAFTAYVRKGDAAKLTQLEGLALEGKALSV
                     GSEADGGYLVPEATERAILKALADLSPIRGIAGHITISSSVYKRPFAVSGANAGWVGE
                     TAPRPQTAGPSLAELSFPTREL"
     gene            1298..2584
                     /locus_tag="VF226_03205"
     CDS             1298..2584
                     /locus_tag="VF226_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7073024.1"
                     /translation="MTVGILGLGYVGLPLALAAVNAGFKVIGFDIDRQRVAQISRGEC
                     AIRHIDTTAIRAALAQGTFRATSDFDGLGTPDAILIAVPTPLNRNREPDLTFVENSTR
                     EIAKRLRPGQLIVLESTTWPGTTAEIMRPILEAGGLVCGKDFYLAYSPEREDPGNPQY
                     ATASIPKVVGADDAASLRAADALYQALVTRTVPVSSAATAEAVKLTENIFRAVNIALV
                     NELKVIFERMNVDIWEVIEAAKTKPFGYMPFYPGPGLGGHCIPIDPFYLTWKAREYDV
                     TTRFIELAGEINTAMPHYVVERLAEALDRRSGRGLNGARILIVGLAYKKNVEDPRESP
                     SLKLMELIERRGAQVDYHDPFIAAIPNTRAYPNFAGRPSQPLTPQALAEYDAVLIATD
                     HDDIDWSGLVANARLVIDTRNVCQRNGIKAEHVVKA"
     gene            2794..3117
                     /locus_tag="VF226_03210"
     CDS             2794..3117
                     /locus_tag="VF226_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073025.1"
                     /translation="MADDRLQRDRSRRNGPYYNGWWIVLLIIIALFVWWGWGWSWWGT
                     PEPETSAGAPQTTTGQPPSGAGGAQAPAPRTEAPKTETPGAQPGTTPGGTTPGPDSGN
                     APPAK"
     gene            complement(3349..4038)
                     /locus_tag="VF226_03215"
     CDS             complement(3349..4038)
                     /locus_tag="VF226_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEX7073026.1"
                     /translation="MAEVSHIRARRENWTRGGPVVTQLDESEIAAVEERATASIADPG
                     PLGLWALATGSWIFGVTVAAFPQSTLTATIPILLLFAGVAQFIAGLYGFRRTNVLMAT
                     AFCSFGALYAVLGLFSAMDASNSLAGVADAPVIQGFLLVSFGFISLALAIAAMRTNAV
                     LVAVFATLCVGFTLIGISHFAGAAEGWGVLSTIGGWFLCASAVFAYYAGMAMVVNSTW
                     QRTAMPMGGQP"
     gene            4362..5567
                     /locus_tag="VF226_03220"
     CDS             4362..5567
                     /locus_tag="VF226_03220"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7073027.1"
                     /translation="MELVLSALLLFSVAALLARAWRQRSYFRVLDREPAPREPTPSVT
                     VVIPARNEASKIGACVEGLTRQSYPRARFRIIVVDDSSTDGTRSVVAAIARTCPIVSV
                     VSAPPLPAGWTGKSHACWVGAQIETDTEWLCFIDADTRHRPELLASTLAAAQRNDLDF
                     LSLVPRQELGSFAERLIMPCGFYLLAFLQDVREPLDQESEKTTACGQFILVRRFAYLD
                     VGGHSAVAGALSEDVALARAMKRSGWRTAVFGGERLLSARMYDGYASLRAGLTRNLVD
                     MLGGPARALITASAGILLAWACLLLPLSTVVTWSPTAADTVSILLAWSAAAAAFAFHI
                     AGARHFGIPAWYGLLFPVGYSVGAGLVIESVWRRRTGRVIWKDRTYPGESQTELRSTS
                     ASYSCSQGS"
     gene            5564..6616
                     /locus_tag="VF226_03225"
     CDS             5564..6616
                     /locus_tag="VF226_03225"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7073028.1"
                     /translation="MTEHLRRISGRSMMLVVLVIGVAAYAARPILLPFVAAGVLAFVC
                     TPLVNWISSRTGIWRALAAAVVFISVLAIVGAFGYLALPRVLSQALTVVSNLQEIIQS
                     AIAGVIGDQPVSILGQIVNPQELAQRTVESIRNWIGQPERLLTIAGLSFVSMFGMILT
                     VVLFAYMLFDGPGIERGLLWLVPPQQRSFARHLWERVEPALRRYFVGLAVVVLYTSAA
                     AYIGLAIFLHLPHAVLLAIATGFLELIPFIGPLAAAVLAGMIALQNAHGIESVVAYAL
                     YASALRLSIDQIVGPVVLGTAARIHPALVIFCLLVGGYLFGVIGIVLAIPVAVTIKIT
                     LAALYEPLTNEDDAGH"
     gene            6739..8007
                     /locus_tag="VF226_03230"
     CDS             6739..8007
                     /locus_tag="VF226_03230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7073029.1"
                     /translation="MFRPISIRQRIFDDLRRRLGGTVPLRVVLWDGEAFAFSPSPAVT
                     ITLGSRRLVRALMAGNFVRLGDAYVSGELAVDGKVEDIIRVGIELSERIGATPLPSRV
                     ARALAYVSVRHSRRRDKAAIRHHYDVSNDFYRLWLDRNLIYSCAYFKTGSEDLDTAQE
                     QKLEHICRKLLLKPGERLLDIGCGWGGLLNWAATHHGVEGVGVTLSEQQYLLARQRIA
                     EAGLADRVEIRLQDYRDIPGRDEFDKVVSVGMYEHVGLANLPLYFATIVRLLKPGGAV
                     LNHGITLAGGVGRAKGLSGGEFIDNHVFPGGELPPLSRVVKEVAASGLEFVDAEDLRP
                     HYALTLKHWVRRLEACATEATFASSPEHYRIWRIYMAAMAFAFDRGWLSVAQVLALKP
                     YARAMAKRPWTRGYQYDGNANPPSTGSLEW"
     gene            8360..8683
                     /locus_tag="VF226_03235"
     CDS             8360..8683
                     /locus_tag="VF226_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073030.1"
                     /translation="MRELARYLESRPDLADIEAVRAEMALGPPEQTPTIIQLSARYGF
                     EPVAQPSVRRRGGALHRMGENILITMLVLARNPSAWGWDDLARDRAEVFISRSKLRAL
                     HGTTD"
     gene            8792..9937
                     /gene="hpnI"
                     /locus_tag="VF226_03240"
     CDS             8792..9937
                     /gene="hpnI"
                     /locus_tag="VF226_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEX7073031.1"
                     /translation="MLHQVLSNVCLAAAILGCIYLLMSAILVRRFAAQGTRSASRPVP
                     VSILKPLHGVELRLFELLASFCRQNYSAPIEIICGVADRDNPAAAVMKRLAQSYPACK
                     IRCSVSIGSHGSNGKVSNLIGLSKLAAHDVLVISDCDIEVGPDYLSRVIGELERPGVG
                     AVSCLYHGIGGEGLWSQLSALAINLHFLPSVVVAITYGLAEPCFGSTIALKRKTLDDI
                     GGLESFADFLADDYAIGAAVRERGLKVAIPAFTIGHHCYERSLRDLFLHEVRWARTVR
                     IIDPIGYAGAFVAHPLPLAMIGLFSDASAALMLITAAIICRLVLCFSVQRAFGLPRQR
                     YWLLPVRDLLSFAVFVWGCFGATVRWKGTEYQVRSDGTLIPNRSGTE"
     gene            10131..11081
                     /gene="ispH"
                     /locus_tag="VF226_03245"
                     /gene_synonym="lytB"
     CDS             10131..11081
                     /gene="ispH"
                     /locus_tag="VF226_03245"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7073032.1"
                     /translation="MRLVLAQPRGFCAGVIRAIEIVNKALEKYGPPVYVRHEIVHNRH
                     VVESLQAKGVRFVEDLSEVPDGAVTIFSAHGIPRDVEEEARARGLPTIDAVCPLVKKV
                     HNQGRHYVAHGRTLILIGHAGHPEVIGTLGQIDGPKYLVQTEADVEALPVPTDAPVAY
                     VTQTTLSVDDTSNIIAALKRRFRDIVGPETRDICYATQNRQTAVRQLARIADVILVVG
                     ARNSSNSNRLREIGEECGVRSYLIANGDELRPEWLVGADVVGLTAGASAPEVLVESVI
                     ERLRSIAPLEVTTMAGVEENITFRLPVQLARDAARTSAGV"
     gene            11090..12247
                     /gene="hpnH"
                     /locus_tag="VF226_03250"
     CDS             11090..12247
                     /gene="hpnH"
                     /locus_tag="VF226_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX7073033.1"
                     /translation="MGIPARQLIKVGSYIARQHLAGRKRYPLVLMLEPLYRCNLACAG
                     CGKIDYPEPILNQRLSVEDCLGAVDECGAPVAVIAGGEPLLHKELPQIVDGILNKGKI
                     VIVCTNGLLLEKQLDKFKPDPSFTWSVHLDGDEEMHDRSVCQKGVYARAVSAIRAAKA
                     KGFRVTINATFFKGADPARIARFFDHVTALGIDGITMSPGYAYERAPDQQHFLTRTAT
                     KGLFRSIFRHGDGGRRWSFNQSSLFLDFLAGNQEYRCTPWGNPTRTVFGWQRPCYLLG
                     EGYAKTFKELMEETNWDDYGTGNYEKCADCMVHSGYEASAVMDAVRHPLKAMRVAVGG
                     VRTEGPMAPEISLSGQRPAQYVFSRHVSQKMSEIREAESRAERRPSTAERA"
     gene            complement(12185..12880)
                     /locus_tag="VF226_03255"
     CDS             complement(12185..12880)
                     /locus_tag="VF226_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase"
                     /protein_id="HEX7073034.1"
                     /translation="MMRPSDAPVLVVTGLLAEARIAAGHGVTVVCRGNQQELLRDIVR
                     RRTDGRFCAVVSFGVAGGLDPDLAPGQIILARQIIRGGERLAADAALTTRVHSKLLSA
                     IPDVRIGDIAGVAEPQCTPAEKQALRSSTGADAADMESWEAALLAAELGVGFAAVRAV
                     CDPAYRRVPAAALAGLGADGGTNAWSVARSLLGTPSELPDLVHCAAGWARALRALRRA
                     RTTLGAGFGLANL"
     gene            complement(12884..>14488)
                     /gene="shc"
                     /locus_tag="VF226_03260"
     CDS             complement(12884..>14488)
                     /gene="shc"
                     /locus_tag="VF226_03260"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX7073035.1"
                     /translation="AILAAGGAARSNVFTRFLLALYGEAPWSSVPEMPVEIMLLPRSF
                     PFHLSKISYWARTVLVPMLVLQALRPRARNRRGVGVRELFCVPPEEVTSWPKGEHRHG
                     GWAVFFDAVDKVLQTLRPAFPARSRQVAIAKAVAFVDERLNGLSGLGAIFPAMANAVM
                     MYDALGVPHTDSRYRLARESIDRLLVERDNEAYCQPCLSPVWDTALSCHALLEVGSRE
                     ARQQALRGLEWLRSRQVLDFAGDWAVQRPTVKPGGWAFQYANPHYPDLDDSAVIAMAL
                     HRWDRTSGEPARFAESVGRAREWIEGMQSRNGGWAAFDADNTYFYLNYIPFADHGALL
                     DPPTADVSARCICLLGQLGDRPHDSPALKAGLAWLRSQQEKNGSWFGRWGVNYIYGTW
                     SALDGLAATGATRETPEVARAVGWLLSIQNEDGGWGEGCESYRLDYAGHEPAPSTASQ
                     TAWALLGLMAAGASDEPAVAKGVGYLLRTQGEDGLWPEQRFTGTGFPRVFYLRYHGYG
                     KYFPLLSLARYLNLIGERANRATSGV"
BASE COUNT         2612 a         4576 c         4542 g         2758 t
ORIGIN      
        1 agctcccgcg tcgggaacga cagctcggcg agcgacggcc ccgccgtctg cggccgcggc
       61 gccgtctcgc cgacccagcc ggcgttcgcc ccggagacgg cgaacggccg cttgtagacg
      121 ctcgacgaga tggtgatgtg gccggcgatg ccgcggatcg gggagaggtc ggcgagcgcc
      181 ttgaggatcg cgcgctccgt cgcctcgggc acgagatagc cgccgtcggc ctctgagccg
      241 accgagagcg ccttgccttc gagcgcgagc ccttcgagct gggtgagctt cgccgcgtcg
      301 cccttgcgca cataggcggt gaacgctgct ttgtgggtgt cggcgaccgt ctcgccaggc
      361 cgcaccagcc ggcccttgtt ggcgagataa tcgatctgct gcttctgctc gtcgagagcc
      421 gcgtcgatgc gggccagctt ctcttccgtg atcacatcgg cgcttttcgc ttccagctcg
      481 gccaggcgcc ggtcattggt cgccttgtac tcgtggaagg cccgcatgaa gtcgtcgaag
      541 gccatcgaga tctgatacat gctgggggac tccgggcttt cggtgatttc ggtttcggac
      601 ccggacactt cgtcaagact catcttgcat ttccttcttc acgtttagcc ccgtggatcc
      661 cggagcccgg cgaaaccgca gagcaccaga attcaccaga ccatcatcac cccggtcgga
      721 aggggcgggg ataactttgg gcgagggctt tttgcccaac gtggtaaacg gagggaattc
      781 ggaccggttt ttcagcgcat taggctttcg cgccggacgt ttatggtttt gattgaatcc
      841 acgacttcgg cttcggcccc gacataggat ggaacaagcc caagccaagc tcgcgcccac
      901 tttccctctc cccgctgggg agagggtcag ggtgaggggg ggggcgccac tggcaattgt
      961 ttgcaagccc tcaccaggcg cttcggactg accccggatc aaatccgggg caggctctct
     1021 ctcaatagcc ccgctgcaat cctccgagcg gtatcgatcg ccatttgtgg aatgttcctc
     1081 aatcccccac gctgacgctt gccgccggcc cgccaccgag cgcagtatgc gcgagcttgc
     1141 tatcggcccg ttgaagggtc agccgttttg aaaccggctc gcagccatcc atttctcgaa
     1201 gaatactcga caagcgaaag ccaaagccat gatcgccgga accgaccaca cccaatccga
     1261 tgtcggccac ttctgcgcac gtgttgcggg ccgtgatatg accgtcggca tcctggggct
     1321 cggctatgtc ggattgccct tggccctcgc cgccgtaaac gccggattca aggtcattgg
     1381 cttcgacatc gatcgtcagc gggtggcgca gatctcgcgc ggggaatgcg cgatccggca
     1441 tatcgatacg acagcaatcc gcgcggcgct ggcacaaggc acattccggg cgaccagcga
     1501 tttcgacggg ctcggcacgc ccgatgccat cctgatcgcg gtgccgaccc cgctcaaccg
     1561 caatcgcgag cccgatctca cgttcgtcga gaacagcacg cgcgagattg cgaaacgcct
     1621 gcgcccgggg cagctcatcg tgctcgagtc gacgacctgg cccgggacga cggctgagat
     1681 catgaggccc attctggagg ccggcgggct cgtgtgcgga aaggacttct atctcgccta
     1741 ctcgcccgag cgcgaggacc cgggcaaccc gcagtacgcg accgcgagca ttcccaaagt
     1801 ggtcggcgcg gacgatgccg cctccctgag ggcagccgat gcgctctacc aggccctcgt
     1861 gaccaggacc gtgccggtgt cgtcggcggc gaccgcggag gccgtgaaac tgacggagaa
     1921 tatcttccgg gcggtcaaca tcgcgctggt gaacgagctc aaggtcattt tcgagcggat
     1981 gaacgtcgac atctgggagg tgatcgaggc ggcgaagacg aagccgttcg gctacatgcc
     2041 gttctatccc ggccccggtc tcggcggcca ctgcattccg atcgatccat tttatctgac
     2101 gtggaaagcc cgcgagtacg acgtgaccac ccggttcatc gagctcgctg gcgagatcaa
     2161 cacggccatg ccgcattatg tggtggagcg tctcgccgaa gcgctcgacc ggcgcagcgg
     2221 ccgcggcctc aatggggcgc gcattctgat cgtcgggctc gcctacaaga agaacgtcga
     2281 ggacccgcgc gaaagcccgt cgctgaagct catggagctg atcgagcggc gcggcgcgca
     2341 ggtcgactat cacgatccgt tcattgcggc cattccgaac acccgtgcgt atcccaattt
     2401 cgcgggccgg ccgtcgcagc cgctcacgcc acaagcgctt gcggaatatg atgcggtcct
     2461 catcgcgacg gaccacgacg acatcgactg gtcagggctc gtcgccaacg cccgcctcgt
     2521 catcgacacc cgcaacgtgt gtcagcggaa cggaataaaa gcggagcatg tggtgaaggc
     2581 gtgagcgtta gcgtgaccca ttgccccaat tgggcggctg agaaattcac gctcaattag
     2641 ccctgtgcgg tggtttccgt ccgctgttgg ccgctgcgct tcagctgctc catcggcgac
     2701 cgttttcctc gatgccgacc cagcagggaa cgagccagga gccgtcgagt tgaacgcctg
     2761 tatgacggtg gataactcac ctcgagagca gccatggcag acgatcgact gcagcgcgac
     2821 agatcccgcc ggaacggccc gtactataac ggctggtgga tcgtccttct gatcatcatc
     2881 gccctcttcg tctggtgggg ctggggatgg agctggtggg gcaccccgga gcccgagacg
     2941 agtgccggtg ctccccaaac cacgaccggg cagccaccgt caggtgctgg cggcgcacaa
     3001 gcgcctgcac ctcggaccga agcccctaag acggaaacgc caggcgcgca acctggcacg
     3061 acgcctggtg gcacgacccc tggtccggac agcggcaacg ctccgccagc caagtgaatt
     3121 ggtgtccgcg ggcggattct gtcgagcccg agcattttca gcacccccgc cgtcgctttc
     3181 gtaaagggcc gccgaaagca aaatcgtgct ttcgaatgca tgccttcaag ccgaatactc
     3241 gaaacgggcc atccgacccg acctccccgc gtctggctcg cggcaccggg tatgaatggc
     3301 ccgatcaaaa agtcgcctgg cggcgcggcc cgccgacccg cgcacatctt agggctgtcc
     3361 gcccatcggc atagccgtcc gctgccacgt gctgttcacg accattgcca tgccagcata
     3421 gtaggcgaac accgccgatg cgcagaggaa ccagccgcca atggtggaca ggacgcccca
     3481 gccttctgcc gcgccggcaa agtgagagat gccgatgagc gtgaacccga cgcacagggt
     3541 ggcgaagact gccaccagca ccgcgttggt ccgcatggcg gcgatggcga gcgccaggga
     3601 aatgaacccg aacgagacga gcaggaatcc ctggatcacc ggagcatcgg cgacgccggc
     3661 caggctgttg ctggcatcca tcgcggagaa cagcccgagg acggcgtaaa gcgcgccaaa
     3721 cgagcaaaat gccgtcgcca tcagcacatt ggtgcggcgg aagccgtaaa ggccggctat
     3781 gaactgcgcg acgccggcaa acaatagcag gatcgggatc gtggccgtca gcgtcgattg
     3841 cgggaaggca gcgacggtta ctccgaatat ccagcttccg gtggccaacg cccacaatcc
     3901 caatggacca ggatcggcga tactcgctgt cgcccgctcc tcgacggcgg cgatttcgga
     3961 ttcgtccaac tgcgtgacga cgggcccccc tctggtccag ttttcacgcc ttgctctgat
     4021 gtgactgact tcagccatgg taagctccat tcaccgttaa ggattttgct taatccctga
     4081 ctgatcaacg cgctgaggcg tagaaagtct cgtgggttcc gagcgcgagg acgcggcttg
     4141 ttgcagcact caagcccccc atgagcgagg gcgagcatcg gcccatgcgg cccgtaatca
     4201 acgcgtgggc gctgagcccg gcgatcgttt gccggccagg gccgataggg attgcacctg
     4261 ttgcatcggg cgggcatcgg aacattcgga agcctcgccg gttgagccgg atgcatcgca
     4321 ggctcgcccg attgagcctg ggagcatcaa ccggatttgc cgtggaactg gttctttctg
     4381 cgctattgct gttctcggtc gccgcgctgc tcgcacgtgc ctggcgccag cggtcctatt
     4441 tccgggtgct cgatcgggag ccggcgcctc gcgagcccac accgtctgtg accgtcgtca
     4501 ttcctgcacg caacgaggca tcgaaaatcg gagcctgcgt ggagggattg acccgccagt
     4561 cctatccgcg cgcgcgtttc agaatcatcg tggtggatga cagttccacc gacgggacac
     4621 ggtctgtcgt ggctgcgatt gcccggacct gccccatcgt ttccgttgtg tccgcgccac
     4681 cgctgccggc tggctggacc ggaaaatctc atgcctgctg ggtcggcgcc cagatcgaga
     4741 ccgacacgga gtggctgtgc ttcatcgacg ccgacacccg gcaccgtccc gagcttctgg
     4801 ccagcactct tgccgcagcg cagcgaaacg atctggattt tctttcgctg gtgccgcggc
     4861 aggaactcgg gagcttcgcg gagcgcttga tcatgccgtg cggattttat ctgctggcat
     4921 tcttgcagga cgtgagggag ccgctcgatc aggaaagcga gaaaacgacc gcctgtggcc
     4981 agttcattct ggtgcgacgc ttcgcttatc tggacgtcgg aggccactct gcggtcgccg
     5041 gcgccttgag cgaggacgtg gcactggccc gagcgatgaa acggagcggc tggcggacag
     5101 cagtattcgg aggcgagcga ttgctcagcg cgcgcatgta cgacggctac gcgagcctgc
     5161 gggccggcct gacgcgcaat ctggtcgaca tgctgggcgg cccggctcgg gcgctgataa
     5221 cagcgtctgc gggcattctc ctggcctggg cgtgcctcct tctaccgctg tcgactgtgg
     5281 tgacctggag cccgacagcg gctgacactg tttcgatcct ccttgcctgg agcgccgcgg
     5341 ctgccgcttt cgcgttccat atcgcgggcg cgcgtcattt cggcattccg gcatggtacg
     5401 gccttctttt tccggtcggc tactcggttg gagctgggct cgttatcgag agcgtctggc
     5461 gaaggcggac ggggcgcgtc atctggaagg atcgcaccta tcccggtgag agccagacgg
     5521 aactacgctc gacctcggcc tcctattctt gctcacaagg gtcatgaccg agcacctgcg
     5581 tcgcatttcc gggcgcagca tgatgctcgt cgtcctcgtg atcggggttg cggcgtatgc
     5641 cgcgcggccg atcctgctgc cgttcgtcgc cgccggggtg ctcgcgttcg tctgcactcc
     5701 gctggtgaat tggataagct cgcggacggg gatatggcgc gcactcgcag ccgccgtggt
     5761 gttcatcagc gtgctcgcca ttgtcggcgc cttcggttac ctggcactcc ctcgtgtcct
     5821 gagccaggcg ttgacggttg taagcaacct tcaggaaatc attcagtctg ctattgccgg
     5881 cgtaatcggt gaccagccag tcagcatact tggacagatc gttaatccgc aggaactggc
     5941 tcaacgaact gtggagtcca ttcgaaactg gatcggccag cctgagcgct tgttgaccat
     6001 tgccgggctg agcttcgtct cgatgttcgg gatgattctg accgtcgtac tcttcgcgta
     6061 tatgcttttc gacggaccgg gcatcgaacg gggcctgctc tggctggtgc ctccgcagca
     6121 acgatccttt gcccgtcatt tatgggagag ggtcgagccg gcgctgcgcc gttatttcgt
     6181 cggcctggct gttgtcgtcc tctatacgtc ggccgccgcg tatatcgggc ttgcaatttt
     6241 tctgcatctt cctcacgcgg ttctgcttgc cattgcgacg gggtttctcg aattgatccc
     6301 cttcattgga ccgctggcgg ccgccgtcct ggccgggatg atcgcgttgc agaatgctca
     6361 cgggatcgaa agcgtcgtcg cttacgcgct ttatgcatcg gcattgcggt tatcaatcga
     6421 tcagatcgtc ggaccggtcg tgcttggcac ggctgcgcgc attcacccag cccttgtcat
     6481 cttctgcttg ctcgtcggcg gctatctgtt cggcgtgatc gggatcgtgc tcgccatacc
     6541 ggtcgcagtc acgatcaaga tcacgctggc cgcgctttat gaaccgctca cgaatgagga
     6601 cgacgccggt cattgagccg gtgccgacct cagaactatt tcccgcctcc tttgttgatc
     6661 atcgtgcgag ccacgagaaa gctcgctcgc gatagaccct cgaactctgg cgaacttcgg
     6721 actgaggtca cgagcgggtt gttccgtccg atatccatac gccagcgcat tttcgacgac
     6781 ttgcgacggc ggctcggggg aacggtgccg ttgcgggtgg tgctctggga cggcgaggcg
     6841 tttgctttct cgcccagtcc ggccgtgacc atcacgctcg gatcgcgacg cctcgttcgg
     6901 gcgctgatgg ctgggaattt cgtccggctt ggagacgcct acgtcagcgg cgagctcgcc
     6961 gtcgacggca aagtcgagga catcatccgt gttggcatcg agctttcgga gcgcatcggc
     7021 gcgacgccgt tgcccagccg tgttgcacgg gcgcttgctt atgtttcggt ccgccacagc
     7081 cgccggcgtg ataaggctgc catccgccat cactacgacg tctccaacga cttctaccgc
     7141 ctctggctgg accggaacct gatctactct tgcgcctatt tcaaaacggg atcagaggat
     7201 ctggataccg cccaggaaca gaagctggag cacatttgcc gcaaactgct tctgaaaccg
     7261 ggtgagcggc tgctcgacat cgggtgcggt tggggaggtc ttttgaattg ggctgcgacc
     7321 caccacggtg tcgaaggcgt cggcgtaacg ctcagcgagc agcagtactt gttggctcgc
     7381 cagcggattg ccgaggcggg gttggcagat cgcgtcgaga tccgcctcca ggactatcgc
     7441 gatattccag gcagggacga gttcgataag gtggtctccg tcggaatgta cgagcacgtc
     7501 ggccttgcca acctgccgct ctatttcgcc acgatcgtcc ggctactcaa accagggggt
     7561 gccgttctta accatggcat tacgttggct ggcggggttg gccgcgccaa ggggttatcc
     7621 ggcggcgaat tcatcgataa ccatgttttc ccgggaggtg agttgccgcc tctttctcgc
     7681 gtcgtcaaag aggttgcagc cagcggattg gagttcgtcg acgccgagga tttgcgccct
     7741 cattatgcgt taaccttgaa gcactgggtc cggcgcctcg aggcttgcgc cactgaggca
     7801 acatttgcgt ctagtccgga acattaccgt atctggcgca tttatatggc tgccatggct
     7861 ttcgcatttg accgcggctg gctctcggtc gcccaggtcc tcgccctgaa accgtacgca
     7921 agggcaatgg caaagcggcc gtggacccgc ggctaccaat atgatggcaa cgcaaatcct
     7981 ccgtcgaccg gcagtctgga gtggtgagat gagatggacc gggtcggttg ggcaaccgtc
     8041 gacgcacgca tcgggagggg aaaagccggt cacgccagaa gttgcaaagc ccaacctgtt
     8101 taccaaagcc gtttaccgtc tggatgccct aattcgcaac cgacacggca tattcgaata
     8161 ctccaatcac cctgattgct tattccggat gcatatcgga aacctggaca gaaacattcg
     8221 gctcctggac gggacttgcc ttgccgaggg tagccggatt gccatcctcc acgtttggaa
     8281 tgagcaagtc ccacaatttc cgaaaaaggg acccaatctg cagtgggcgc gctcgctttc
     8341 gcggaagctc agcttttcca tgcgcgagct ggcccgctat ctggaaagcc ggccggatct
     8401 ggccgacatc gaagcagttc gggccgaaat ggcccttggc ccgccagagc agacgcccac
     8461 catcatccag ctttcggccc ggtacggctt cgagccagtg gcgcagccct ccgttcggcg
     8521 ccgtggcgga gcactgcacc ggatgggaga aaacattctg atcaccatgc ttgtgcttgc
     8581 cagaaatccc tctgcctggg gctgggacga tctcgccagg gacagagccg aggtcttcat
     8641 ctcccggtcg aagctgaggg ccctccacgg gacgaccgac tgattcgcag ccggggaggg
     8701 gagtcgaacc attcgctcgg gccggcgttc tcagtcgtca gtctcgatca gcgaaggccg
     8761 gtggtccggt ccacagcaag cgggccggcg catgctccat caggttctgt caaatgtatg
     8821 cctcgccgcg gcgatcctcg gttgcatcta tctgctgatg tcggcgattc tggtgcgccg
     8881 gtttgcggcc caggggacgc ggtcggcaag tcgaccggtt ccagtttcga tcctgaagcc
     8941 gctgcacggg gttgagctcc gcctcttcga gctattggca tccttctgcc gccaaaacta
     9001 ctcggcaccc attgaaatca tctgcggtgt cgccgaccgg gacaatccgg ccgcagcggt
     9061 catgaagcgg ctcgcccagt cctacccggc gtgcaagatc cgttgttccg tctcgatcgg
     9121 gagccatggc agcaatggaa aggtttcgaa cctcatcggc ttgtccaagc tggcggcaca
     9181 cgacgtgctg gtcatctcgg attgcgatat cgaggtcggg ccggactatc tgtcccgggt
     9241 gatcggtgag ctggagcggc ccggcgtcgg cgccgtctcc tgtctttacc atgggatcgg
     9301 cggggaaggg ctctggtccc agctttcggc tctggcgatc aatctgcatt ttctcccgag
     9361 tgttgtcgtc gccatcacgt acgggctggc ggagccctgc ttcggctcca cgatagctct
     9421 taaacgcaag acactcgatg acattggagg gctcgagagc ttcgccgatt tcctcgctga
     9481 cgattacgcg atcggtgctg cggtgcgcga acgaggtctc aaggtggcga tccccgcctt
     9541 caccatcggc catcactgct acgagcggag cctgcgcgac ttgtttctcc atgaagtccg
     9601 atgggcccgg acggtccgga tcattgatcc gatcgggtat gccggagctt tcgtcgctca
     9661 tcctctgccg ctggccatga tcggcttatt ttccgatgcc agcgctgcct tgatgctgat
     9721 aaccgccgcg atcatctgcc ggctcgttct ttgcttcagt gtccagcggg cgttcggact
     9781 gccgcggcag cgctactggc tgcttccggt ccgcgatctg ctctctttcg cggtattcgt
     9841 ttggggctgc ttcggcgcca cggtgcgctg gaagggcacg gagtatcaag tccgatccga
     9901 tggcaccctg attccgaaca ggagcggaac cgagtaacgg atccgggcgg ccgcgcgata
     9961 gcttgaactg aggagtttac ctcggggaca gccaaaggac cgatgctccg acgcaatcgt
    10021 gaaacgaatg ccgttccggc acgttcagtg gcagcgggat gggagacatt ccgctgatga
    10081 aggactggca gatcgcacgc tttttgcgat accaccggac tggatacggc atgagacttg
    10141 tgcttgctca gccccgcggc ttttgcgccg gagtcatccg cgcgatcgaa atcgtcaaca
    10201 aagcactcga gaagtacggc ccgccggtct acgttcgcca cgaaatcgtg cacaaccgcc
    10261 atgttgtcga aagcctgcag gccaagggcg tccgtttcgt cgaggacctg agcgaggttc
    10321 cggacggagc cgtcacgatt ttcagcgccc acggaattcc gcgtgatgtc gaggaggagg
    10381 cgcgggcgcg cggactgccg acgatcgatg cggtatgccc gctcgtgaag aaagttcaca
    10441 atcagggccg gcattacgtc gcacacggtc gcacgctgat cctgatcgga cacgctggcc
    10501 atcccgaggt gatcggaacg ctcggccaga tcgacggccc gaaatatctc gtgcagacgg
    10561 aagcggatgt cgaggcgctg ccggtgccga cggacgcccc ggtcgcatat gtgacgcaaa
    10621 cgacgctcag cgtggacgac acgagcaaca tcatcgcggc actgaaacgg cgcttccgcg
    10681 acatcgtcgg ccctgaaacg cgcgacatct gttatgcgac gcagaaccgc cagaccgccg
    10741 tgcgccagct cgcccggatt gcggacgtca tcctcgttgt gggagcgcgc aacagctcca
    10801 actccaatcg cctccgtgaa atcggcgagg agtgcggcgt gcggagttac ctcatcgcga
    10861 atggcgatga actccggccg gaatggctag tgggcgccga tgtggtcgga ctgaccgctg
    10921 gggcgtcggc tcccgaagtc ctggtcgaga gcgtcatcga aaggctgagg tcgattgctc
    10981 ctctcgaggt gaccacgatg gccggagtgg aggagaacat cactttccgc cttcccgttc
    11041 agcttgcgcg cgacgccgcc cgcacgagtg ccggcgtgtg aggccgtcca tgggcattcc
    11101 cgcgcgacag ttgatcaagg tcggcagcta tatcgcccgc cagcacctcg ccgggcgcaa
    11161 gcgctatccg ctggttctca tgctcgagcc gctctaccgg tgcaatctcg cctgcgcggg
    11221 atgcgggaaa atcgattatc ccgagcccat tctgaatcag cggctgtccg tcgaggattg
    11281 cctcggcgca gtcgacgaat gcggtgctcc cgtcgccgtg atcgcaggcg gcgagccctt
    11341 gctgcacaag gagttgccgc agatcgtcga tgggatcctg aacaagggca agatcgtcat
    11401 cgtttgcacg aacggcctgc tgctcgaaaa gcaactcgac aagttcaaac ccgacccttc
    11461 cttcacctgg tccgtgcacc tcgatgggga cgaggagatg cacgaccgct cggtgtgtca
    11521 aaagggcgtt tatgctcgtg ccgtctcggc gatccgagcc gccaaggcca agggctttcg
    11581 ggtgacgatc aacgcgacgt ttttcaaagg ggccgatccc gcccgtatcg cgagattctt
    11641 cgaccacgtg accgcactcg gcatagacgg catcaccatg tcgccgggat acgcctatga
    11701 gcgcgcgccc gaccagcagc attttctgac ccgtaccgcc acaaaggggc tgttccgctc
    11761 gatcttcagg catggcgatg gcggtcggcg ctggagcttc aaccaatcga gcctctttct
    11821 cgatttcctt gccggcaatc aggagtaccg ctgcacgccc tggggtaatc cgacccggac
    11881 ggtgttcggt tggcagcgcc cttgctacct tctcggcgag gggtacgcga agacgttcaa
    11941 ggagctgatg gaggagacga attgggatga ttacggcacc ggcaattacg aaaaatgcgc
    12001 cgattgcatg gtgcacagcg gatacgaggc gagtgcggtg atggatgccg tccgtcatcc
    12061 gctcaaggcg atgagggtcg cggtaggcgg agtacgcacg gaaggcccga tggcgccgga
    12121 aatcagcctc agcgggcagc gcccggcgca gtacgtcttt tcgcgccacg tctcccagaa
    12181 aatgtcagag attcgcgagg ccgaatcccg cgccgagcgt cgtccgagca cggcggagcg
    12241 cgcgtaacgc cctggcccat ccagccgcac agtggaccag atccggaagc tcactcgggg
    12301 tgccgagcag gctgcgtgcg acgctccagg catttgtgcc gccgtcagcg ccaagcccgg
    12361 cgagcgcagc agcggggacc cggcgatacg ctgggtcgca gaccgccctc acggccgcaa
    12421 aaccgacacc gagctcggcc gcgagcagag cggcctccca ggattccatg tcggcggcat
    12481 ccgcgccggt tgatgagcgc aatgcctgct tttccgcggg tgtgcattgc ggctcggcca
    12541 ctccagcaat gtcgccgatc cgaacatcgg gaattgccga taacagcttc gaatgcaccc
    12601 gggtcgtcag cgctgcatct gccgccagcc gctcgcctcc gcgaataatc tgccgggcta
    12661 atatgatctg gcccggcgcg aggtcagggt cgagcccgcc tgcgaccccg aagctgacca
    12721 cggcacagaa acggccatct gtcctgcgcc gaacgatgtc ccgcaggagc tcctgctgat
    12781 tgccgcgaca aacgacggtg acgccgtggc cggcggcaat gcgagcctct gcaaggagcc
    12841 ccgtgacgac cagaaccgga gcatcgctgg gccgcatcat gcgtcacacg ccggacgtcg
    12901 cgcgattagc cctctcacca atcaaattga gataacgcgc gagcgaaagc agcggaaaat
    12961 atttaccata tccgtgatag cgaagataaa agacgcgagg aaagccggtg ccggtgaaac
    13021 gctgctcggg ccaaaggccg tcttctcctt gcgtgcgcaa gagatagccg acacccttcg
    13081 caacggcagg ttcgtccgat gcgccagcgg ccatgaggcc aagcagcgcc caggcagtct
    13141 gggaggcggt gcttggcgcc ggctcgtgac cggcgtaatc cagccgataa ctttcacaac
    13201 cttcgcccca gccgccatcc tcgttctgga tcgacaagag ccatccgact gcgcgggcaa
    13261 cctccggtgt ctcccttgtg gcgccggtcg ccgcgagccc atccaaggcc gaccaggtgc
    13321 catagatgta attgacgccc cagcgaccga accaactccc attcttttcc tgctgcgagc
    13381 gcagccaggc gaggccggct ttcagggcag ggctgtcgtg cggccggtcg ccgagctggc
    13441 ccagcagaca gatgcaacgc gcgctgacgt ccgccgtcgg tggatcgagc agggcgccat
    13501 ggtcggcaaa gggaatgtaa ttgagataga aatacgtgtt gtcggcgtcg aaggccgccc
    13561 aaccgccgtt tcggctttgc atcccctcga tccactcgcg cgcgcggccg acggattcgg
    13621 caaaccgcgc cggttctccg gaggtcctgt cccaccgatg caacgccatc gcgatcacgg
    13681 cactgtcgtc gagatcggga tagtgcgggt tggcatactg aaaggcccat ccgccgggtt
    13741 tcacggtcgg cctttgcacc gcccagtcgc ctgcaaagtc gagcacttgc cgggaccgca
    13801 gccattcgag tcctctgagg gcctgctgcc ttgcctcccg gctgcccacc tcgaggaggg
    13861 cgtgacagct cagcgccgtg tcccagaccg gagagaggca gggctggcaa taagcctcgt
    13921 tatctcgctc gacgagcaga cggtcgatgc tctctcgtgc caggcggtag cgcgaatccg
    13981 tatgcggcac gcccagcgcg tcgtacatca tgacggcatt ggccatggcc gggaaaatgg
    14041 ctccgagccc ggaaagcccg ttgagccgct cgtccacgaa tgcgacagcc ttcgcgatgg
    14101 cgacttgccg cgacctggca ggaaaggccg gccgtaaggt ctgcagcacc ttgtcgactg
    14161 catcgaagaa aacggcccac ccaccgtgcc gatgctcgcc cttcggccag gacgtcacct
    14221 cctcgggagg cacacagaaa agttcgcgga cgccgacgcc tcgcctgttg cgtgcgcgcg
    14281 gccggagggc ctggagcacg agcatcggta cgagcacggt ccgggcccaa taggagattt
    14341 tcgacagatg gaatgggaag gaccgcggga gcagcatgat ttcgaccggc atttccggaa
    14401 cgctgctcca gggcgcctcg ccgtagagtg cgagcagaaa gcgcgtaaag acgttgcttc
    14461 ttgcggctcc acccgcggcg agaatggc
//
LOCUS       DASVDU010000047        11676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1483393, whole genome shotgun sequence.
ACCESSION   DASVDU010000047 DASVDU010000000
VERSION     DASVDU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1483393"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..670)
                     /locus_tag="VF226_03265"
     CDS             complement(221..670)
                     /locus_tag="VF226_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7073036.1"
                     /translation="MVPLIILIFGLGDMSKIVTGWIIVFFVVFFNTFEGTRSVDRDQI
                     AAARLLGASEFVILRTVIIPSALAWVFASLTPAVSFALVGVIVGEFVGAQRGLGKLII
                     EAEARANAAEMMVAIFVMMVVGIILAYGVRKIQAYLLRWQPQFEQQF"
     gene            complement(658..1884)
                     /locus_tag="VF226_03270"
     CDS             complement(658..1884)
                     /locus_tag="VF226_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7073037.1"
                     /translation="MIDSPAAARAPAQNLFRPLDGIRVVEFSQMVMGPTCGLILADLG
                     ADVIKVEPIKGDRSRYFKGPASGFFATYSRNKRSLSVDTGSPAGQEIVRRLIKKSDVL
                     IENFRPGLLARFGLDYDSVSGFAPHLIYCSLKGYLPGPYEHRLALDEVVQMMGGLAYM
                     TGLPDRPMRAGASVNDVMGGMFGVIAIQAALAERQRTGRGRYIQSALFENNVFLMGQA
                     MMFEAVTGTPSIPYSVKDSPWPVYDLFETKDGSKLFVTIVGEEHWEAFCRTFGREQWL
                     EDPRLATNQGRVDQRSWIIPEIASIFRQWTAAELAETLERLQLPYAPVNKPGDLFNDP
                     HLMASGGLMDIRLPDGRTAKTPALPISVDGSRLANRRDPPQLGEHTREILTELGYNDA
                     ECVDLAQSGTIRLWSR"
     gene            2212..3156
                     /locus_tag="VF226_03275"
     CDS             2212..3156
                     /locus_tag="VF226_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073038.1"
                     /translation="MSVHKSSRAEQVANQILLIARREGLEAGERLVEQRLAEALGVSR
                     GPVRAGLKVLAAAGLVTGERNRGYVLAKTPTSAETAITTVDENEMAYRQIADDLLDGR
                     LPEVVMEAELMRRYHLTRAQLMRVLDRVASEGWGTRLQGYGWRFAEILSSNKAYSQAA
                     AFRAVIEIAALSERKYKLDTHVIERLRSQQQSIIDRFQMLTPGEIFQIGCEFHEEIIR
                     GAGNPFYVEALKRVNSIRRLLAYRTYADEVGMRRHIGEHLRLLDLIADGRMTDAAALL
                     RRHLKRPPKPRPGQRRSQQPHHSSDLLSGNGISRQRPA"
     gene            3381..4382
                     /locus_tag="VF226_03280"
     CDS             3381..4382
                     /locus_tag="VF226_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073039.1"
                     /translation="MTMLYAATGDSFARLEEDGGSWRASLSLQDKGVQCLAVDPLNPA
                     IVYAGTRGNGVWRSGDGGTNWRDLALPQPDVFSLAVSAADGALYAGTEPSHLFVSRDG
                     GKTWRELHALLDLPSYPEWRFPPRPWTSHVRSIAPSPHDASLLLVGIELGGLMRSTDG
                     GETWQDHRPGAQKDVHALAWHPNVPGRAYEAAGGGAAWSHDGGDSWAAADAGRDRDYC
                     WGLAVDPDDPDTWFISAAPGPGRAHNAQLAAEAVIYRWRGAGPWEALEGGLPKQPLES
                     MPRALATVPGQVFAGFDDGQIYVSRNGGDNWRALAIDGDRPSRIEALAAVSNSLPSP"
     gene            5648..6520
                     /locus_tag="VF226_03285"
     CDS             5648..6520
                     /locus_tag="VF226_03285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7073040.1"
                     /translation="MNKFIATASRSVIFALVGSGIAALLSGLAIRGALRRLAAAAEQV
                     GESSTFNYRAGLVKEANQVAARLSVAADRITAREQELRTNEERIQTVVRELNHRSKNL
                     LAIIQVIARQSARGCTYASDFLRSFSERLAGLARSQVVLTERGWGAVPIRELVNVHLD
                     PFLKANGRIECSGPDVALEASAAQDIGMVLHELATNAAKYGALSNPSGRIRIEWLKRA
                     DPAGQNGLELRWSETGGPIVTPPTRKGFGQTLLEQVMASIGGSADLDWRKEGLTLTIV
                     LPHNCLREQPGVQQ"
     gene            complement(6741..7073)
                     /locus_tag="VF226_03290"
     CDS             complement(6741..7073)
                     /locus_tag="VF226_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015633514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX7073041.1"
                     /translation="MATFIMLTRVSEQALQSPSSFEKLGRDVSERIRSDCPEVEWKQS
                     YAVLGPADYLDIFTAPDMEAAMKVAAVVRSFGHASTEVWGATEWEQFKNIVAPMAAQQ
                     PATAMASV"
     gene            7310..8767
                     /locus_tag="VF226_03295"
     CDS             7310..8767
                     /locus_tag="VF226_03295"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7073042.1"
                     /translation="MAAIGTAEGVPNFDTRDRVDVAIVGAGTAGLAALREVRDRTNSF
                     VLINGGPYGTTCARVGCMPSKTLIAAANAFYLRTKLDEFGIRGGDRLVADIPAVLHRV
                     RSLRDHFVAGVLKATEDLGDRKISGYARLDGPGRLIVNDRVIQARQIILAPGSSPIVP
                     KPWRALGSRILTSDTLFEQENLPPRVGVIGLGPLGVEIAQALARLGLEVYGFDALERV
                     AGISDEKVSAAALNCLASEFAIHLGAQVDLAEVDDGVEIRWDDKNVVVDKVIVAIGRR
                     PNLEGLGLETLGVPLDEKGMPEVDPSTMRIPGTSVLLAGDANGDRPLLHEAADEGHIA
                     GLNATSDLPIQLPRRTPLSIVFCDPEIATVGQRAKDLDPETILIGEASFAGQSRARIM
                     QENRGLLRLYAARQGGRLLGAEMAVPGAEHMAHLLALAIGQELTVHDLLRMPLYHPTL
                     EEGLRTALRTIARQLPPCALSDLARCGSLEVEALE"
     gene            8916..9593
                     /locus_tag="VF226_03300"
     CDS             8916..9593
                     /locus_tag="VF226_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7073043.1"
                     /translation="MLGNSAVDLTVDIRALTPDELRAATALLAHGMRDNPLHVRVFGT
                     DPERRGRRLIRFLDPLVAYVHAHGAVLGAYVKGELVGVLGMMEPGGCRPALMDALRLA
                     SMIVASNPPDGALRILRWLATWARNDPAWPHWHIGPMTVDPAYRRRGIGRHLMTHCCR
                     QMDALAATAYLETDTKTNVAFYETLGFAVIKHERVLGVPNWFMSRPPSHRKTAPSDAV
                     PTSEIPR"
     gene            complement(9702..10109)
                     /locus_tag="VF226_03305"
     CDS             complement(9702..10109)
                     /locus_tag="VF226_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7073044.1"
                     /translation="MSTKIFVNLPVKDLKKSMAFFEALGFSFNKQFTNDDAACMVISE
                     HNYAMLLTHAYFNKFSVKPIADAHKSTEVLIALALESKAAVDEMVAKAVEAGGKEPRP
                     KQDHGFMVQRTFEDLDGHTWEPFWMDPAHVQAG"
     gene            complement(10140..10601)
                     /locus_tag="VF226_03310"
     CDS             complement(10140..10601)
                     /locus_tag="VF226_03310"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7073045.1"
                     /translation="MFWGGFKPFVGELPIPRIQPYLFFRGRCEEAIDYYKNVLGAEVL
                     MMMRFSDNPDKPGCDVVPAEFDSRIMHASLRIAGTVIMMSDGMKSGPLDFECMSLSLA
                     VSSEAECDRIYNALAADGTVQMPIGPTFFAKRFGAVADKFGVSWMIIVPNA"
     gene            complement(10581..10925)
                     /locus_tag="VF226_03315"
                     /pseudo
     CDS             complement(10581..10925)
                     /locus_tag="VF226_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
     gene            complement(10965..11456)
                     /locus_tag="VF226_03320"
     CDS             complement(10965..11456)
                     /locus_tag="VF226_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7073046.1"
                     /translation="MTARQKIIPCLWFEANAEEAVRFYTSVFPDSAIDRVHLAKADTP
                     GNKEGDVLFIEFTLAGQRYQAINGGAHEKFNDAISLSVDCADQAEVDRYWAALTADGG
                     RPVQCGWLKDKYGLSWQIVPRRLIELLADPDPAKGKRVMQAMMKMVKLDVAALEAAAA
                     ATA"
BASE COUNT         2252 a         3571 c         3586 g         2267 t
ORIGIN      
        1 gaacctgcgc tttgagacgg tggcaattgg cgcgttgccg ttcattgcgg cagacatggt
       61 gcggcttact ctgctggttg cgttcccgtc gttatcattg gccctgcctg cgctgcttat
      121 gtagcacggc actcgctgag cagccttttc gacatttgaa aggtaactgg cggggctcaa
      181 cgagagaccg acggcgtgtg ctgccgctca ttggctcagt ctaaaactgc tgctcgaact
      241 gaggttgcca gcgcagcaga taggcctgaa tttttcggac gccgtacgca agaatgatgc
      301 cgacgaccat catcacgaaa atcgcgacca tcatttccgc ggcgttggcg cgtgcttctg
      361 cctcgatgat cagcttaccg aggccgcgct gagcgccgac gaactcgccg acaatgacgc
      421 cgactagggc gaaggagact gcgggcgtca gcgacgcaaa cacccaggcc agtgcggacg
      481 ggatgatgac cgtgcgcagg atgacgaact cgctcgcgcc cagcagccgc gccgccgcga
      541 tctggtcgcg gtcaaccgac cgcgtcccct cgaatgtgtt gaagaaaacc acgaagaaca
      601 cgatgatcca gccggtaacg atcttcgaca tgtcgcccag cccgaaaatg aggatgatca
      661 gcgggaccaa agtctgatcg tacccgactg agccaagtct acacattcgg catcgttgta
      721 tccgagctcg gtgagaattt cgcgtgtatg ctcgcccaac tgcggcgggt cacggcgatt
      781 cgcgagacgc gatccgtcga ccgagatcgg cagcgccgga gtcttcgcgg ttcgcccatc
      841 gggcaggcga atatccatca accctccgga cgccatcaag tgagggtcgt tgaacaaatc
      901 gccgggcttg ttgaccggtg cgtaaggcag ttgcaagcgc tctagcgttt ccgcgagctc
      961 agcggctgtc cactgccgga aaatgctcgc gatttcggga atgatccatg accgctgatc
     1021 gacccgaccc tggtttgtcg caagccgcgg atcctccagc cattgctcgc gaccaaatgt
     1081 ccggcagaat gcctcccagt gctcctcacc gacgatagtg acaaacagtt tcgatccatc
     1141 cttggtctcg aacagatcat agaccggcca cgggctgtcc ttgaccgagt acgggatcga
     1201 cggcgtcccc gtcaccgcct cgaacatcat ggcctgcccc atcaggaaga cgttgttctc
     1261 gaacaacgcg ctctggatgt aacggccgcg gccggtgcgc tgccgttcgg ccagcgccgc
     1321 ctggatcgcg atgacgccaa acatgccgcc catcacgtcg ttgacggagg cgccggcccg
     1381 catcggccgg tcgggcagtc cggtcatgta ggcgagaccg cccatcatct gcacgacctc
     1441 atcaagggcg aggcggtgct cgtagggccc gggcagatag cctttcaggg agcagtagat
     1501 caaatgcggg gcgaagccgg acacgctgtc gtaatcgagc ccgaagcggg ccaacagccc
     1561 gggccggaaa ttctcgatca gcacatcgga tttcttgatc aggcgacgga caatctcctg
     1621 tcccgccggt gaacccgtat cgactgaaag gctgcgcttg ttgcggctgt aggtagcgaa
     1681 aaagccggac gcgggtccct tgaaatatcg cgagcggtcg cccttgatcg gctcgacctt
     1741 gatgacatca gcaccgagat cggcaagaat cagtccgcac gtcgggccca tcaccatctg
     1801 gctgaactcg acgacgcgaa tgccgtccaa gggacggaat agattttgtg cgggcgcgcg
     1861 ggccgccgca gggctatcta tcaagccacc ctccgctaga ccttgtggat gccggcctga
     1921 cccttgcagg cccgaacgat cctgcgtcga tctcgatcac cccggcgcgg cgatcgcatc
     1981 gatcggagcg ctcttcgatg cagtcggcac gaacgcgccg aggttctgca gcccatcgcg
     2041 caggcttcgt ctcgcaaatg tgcacgtcgg aaaattgagt cacgcgccct tccgtgtcgt
     2101 cgtttcgcta ttgtattttg cgcgagcaaa taacaatgtg gtgcagctgt caacacctca
     2161 gtgcccggct tgcgtgatgg cagatgctac gggccaagtt cggaaccgag gatgtcggtg
     2221 cacaagtcca gcagagcgga gcaggtcgcc aaccagatcc tgttgattgc gcgtcgtgaa
     2281 gggctcgagg ccggcgagcg gctggtggag cagcggctcg cggaagcgct cggcgtctcc
     2341 cgcgggccgg tgcgcgccgg cctcaaagtg ctggccgccg cggggcttgt cacgggcgag
     2401 cgcaaccgcg gctacgtgct ggcgaagacc cctacgtccg cggaaacggc gatcacgaca
     2461 gtggacgaaa atgaaatggc ctacaggcag atcgcggacg acctcctcga tgggcgcctt
     2521 ccggaggtcg tcatggaggc cgagctcatg cgccgctatc atctcacgcg cgcgcagctc
     2581 atgcgcgtgc tcgaccgtgt cgccagcgag ggctggggca cacggctgca aggctacggc
     2641 tggcgattcg ccgaaatcct gtcgagcaac aaagcctaca gccaagctgc agcattccgc
     2701 gccgttatcg aaatcgccgc attatcagag cggaaataca agctcgatac tcacgtgatc
     2761 gaacggctga gaagccagca gcagtcgatc atcgaccggt ttcagatgct gacccccgga
     2821 gagatctttc agatcggttg cgagttccac gaggagatca tccggggcgc cggcaatccg
     2881 ttctatgtcg aagcgctaaa gcgcgtgaac tcgatccggc gactgctcgc ctatcgcacc
     2941 tatgctgatg aagtcggcat gcgccggcac ataggtgagc atttacgcct tctcgacctc
     3001 atcgccgatg gccgcatgac cgacgctgca gcgctactgc ggcgacacct gaaacgcccg
     3061 ccaaagccgc gaccgggaca gcgcaggtca caacagccac atcattcatc cgaccttctc
     3121 tcgggcaacg gcatttcccg ccagcggccc gcctgaattc cattgccaat atcggaactg
     3181 cagaaaatgc ccctgcccgg ctgagcttcc gcttcaacat cctgttcctc tcccacgctg
     3241 ccgccgacag gttcccctga attgcgtttt ggagccaaac gagatcgagc cggttctcaa
     3301 tagaccatcg gcaagggcga aattgaaagc agagtttgcg ccttcgcccc cgctggtgct
     3361 gtcgatacgg aaggcacgcc atgacaatgc tttatgcggc taccggcgac tcattcgcgc
     3421 gcctcgagga ggacggcggt tcatggaggg catcgctgtc gctgcaggac aagggcgtgc
     3481 agtgccttgc ggtcgacccg ctcaatccgg ccatcgtcta tgctgggacc cgcggcaatg
     3541 gcgtgtggag gagcggcgac ggtggcacca attggcgcga tctggcactc cctcagccgg
     3601 atgtattctc gctcgcggtg agcgcggcag acggcgccct ctatgccggc accgaaccaa
     3661 gccatctgtt tgtcagccgc gatggcggga agacctggcg ggagcttcac gctttgcttg
     3721 acttgccctc ctatccagaa tggcgcttcc cgccgcggcc ttggacgagc cacgtgcgct
     3781 cgatcgcacc ctcaccacac gacgcaagcc tgcttctggt cggcatcgag ctgggcggcc
     3841 tgatgcgcag caccgatggc ggcgaaacct ggcaggacca ccgcccaggt gcgcaaaagg
     3901 acgtccacgc gcttgcctgg catccaaatg tcccgggccg agcctatgag gcggctggcg
     3961 gcggcgccgc atggagccat gatggcggtg atagttgggc cgcggcggac gcagggcgcg
     4021 accgggacta ttgctggggt ttggcggtcg atcccgacga ccccgatacc tggttcatct
     4081 ccgccgcacc aggacccggc cgtgcccata acgctcagct cgctgctgag gctgtcatct
     4141 accgctggcg cggcgctggt ccatgggagg cgctcgaagg cgggctgccg aagcaaccgc
     4201 tggaaagcat gccgagagcg ctcgccactg tgccagggca agtgttcgcc ggctttgacg
     4261 atgggcaaat ttacgtcagc cggaatggtg gtgataactg gagggctctt gcgatcgacg
     4321 gcgatcgccc gtcgcgtatt gaagcgcttg cagcagtaag caattcgctg cccagcccgt
     4381 gattttaatt gcagagctgt ttgacgcgaa gctggcaagc agcgcgatcc tgttggctcc
     4441 gagaaaccgg gatcgtcaag tcgtcgagcg atgagtggcg gcttgctgtt ggtcaagacg
     4501 tcgaaggagt cagaggcctc cggtgggcgg tgctgtcaca agtgccggct cgaaatagga
     4561 gatgtttagc tacgcaagcg cttattctta ttgtcgagtc acccccgatt ggaggtggca
     4621 cgatcgatcg agcggtcgat accggcgtaa tgcaaatgcc tgaatcgcgg ggtcgaggca
     4681 gtttagtagt cgcaggcgcg atccgtccca agctccatag gaactgaggt cgcgcgatcc
     4741 cgtacgggcc tgcttcgaaa cgcaccccaa aaccaatgtg cataacaaac gttatgccat
     4801 cacacttggt ccgtgtgatc tcgacatgtc caccgacccc cgacttcagc agaggggcta
     4861 tccggtcgcg gctctgctcg cggtgatgtt tgtcggctcg ctgttgcctt tcttcgcctt
     4921 gatcgtatcg ggtgctcgtc agcatatggc tgaggagagg ggcgagaact ttgctcagct
     4981 cagtcgccag gcaactgttc tggccaaagc cctggatcgc gaattgcgcg aactcttgaa
     5041 gctggcgcaa gtgactgcgc aatcgaaaac actgcggcaa agcgatgtcg cggagcaaac
     5101 gaggtatctt cgcggtgccg ctcagtacac tggggggcac ttcattcttg ttgaccgaag
     5161 ccttcgacag ctcgtcaaca cggcagctcc cgaaggtgcg ccgctgtctc aagctcccga
     5221 tggcgatgcc gcgcatcgcg tcttcgcatc gggggagccg ctagtcaggg atctcgcgac
     5281 cgcccccttt tttgacgagc ccttcttctc gatcagagta cctgtggtgg tcgacaggga
     5341 cgtcgcttac gtgctcgcct atatcccgca gaaggaggcg cttgcggatg tgctggaccg
     5401 gacattccgc ccggagaact ggcttgcgtc catcgttgat gccaaaggca gggttattgc
     5461 tcattccgat aatccgtggc gctatggaac gcgatcggtc tggcagctac cggaggatca
     5521 acctcccggg gcggttctcg agacggtgga cgaggaaggg cgaccggcat tcgttgcgca
     5581 gtctcgatcg caaatttctg gatggcgggc tttcgtctgg cagtcgaagg ctccgcttga
     5641 agcgaggatg aacaaattca tcgcaacagc cagccgctcg gttatttttg ccctcgtcgg
     5701 atcaggtatt gctgcgcttc tctcgggcct tgccatccga ggggcgctga ggcgtcttgc
     5761 agcagcggca gagcaggtcg gcgaatcgag caccttcaac tacagggccg gcctcgtcaa
     5821 agaggccaac caggttgcag ctcgtctttc ggtggcggca gatcgcatca ccgcccggga
     5881 gcaggagttg cggaccaacg aggagcgcat ccaaaccgtc gtgcgtgaac tcaatcatcg
     5941 gtccaagaac ctgctcgcga taatccaagt gattgcgcgt caaagtgctc gtgggtgtac
     6001 ttacgcgagc gactttctcc ggtcgttcag cgaacgtttg gctggacttg cgcggtcaca
     6061 agttgttctg accgaacggg gatggggcgc ggtcccgata agggagctcg tgaatgtcca
     6121 cctggacccg ttcctcaaag caaatggtcg aatcgaatgc agcggccccg atgttgccct
     6181 cgaggcgtcg gctgcgcagg atatcggcat ggtcctgcat gaactggcta caaatgccgc
     6241 caaatacggc gccctttcga atccctccgg ccgaattcga attgaatggc tgaagagggc
     6301 agatccggca ggacagaacg gtctcgagct gcgctggtcc gaaactggcg gaccgatcgt
     6361 gaccccgccc actcgaaaag gcttcggtca aactcttttg gagcaggtga tggctagcat
     6421 tggcgggtcc gccgatctcg actggagaaa ggaaggtctg acgttaacga tcgttttgcc
     6481 tcataattgc ctgagggagc agcccggtgt gcagcaatga accggggttc tgaacccggg
     6541 ggcaacggca catcaggccg cgcggataat ccgcggagaa tggaaggcgc cgaggagcgg
     6601 caggccaccg atgtgagttg gcgttcaact cgcgtcgaaa tcatccggtt ctcatcagtc
     6661 agaaaaggag cggagggcac tggcgcctcc cgagggcctc gcgccacctc cgctgtgagg
     6721 ttcaccgatc cataggtcgc ctagacggaa gccatggcgg tcgccggctg ctgcgcggcc
     6781 atcggcgcaa caatattctt gaactgctcc cattcagtgg caccccatac ttcggtcgac
     6841 gcatggccaa atgaacggac gacagccgca actttcatgg ccgcctccat atccggagcc
     6901 gtgaagatgt cgaggtagtc ggcagggcca agaaccgcat agctctgctt ccattccact
     6961 tcaggacaat ccgagcgaat gcgctctgac acatcgcggc cgagcttctc gaacgagctt
     7021 ggtgactgaa gagcttgctc gctgactcgg gtcagcatga tgaacgtagc catgaagaca
     7081 cctcctctcg ttgtgcagag gggaaacgcc gtgcgtttag gagtggatcc attctctgac
     7141 cgacgcatca gcctccgccg ccggcccttg cggacgcccc aaccccgacg gcaaggcatg
     7201 ggtcgccagc agccgcaact ttgccggtcc ttcaacctct gtcggaacct cggtaaatcg
     7261 tataggttgg accggaactg gaactcacct cagtcaagcg cggtggacta tggcggcgat
     7321 tggcacagct gaaggcgttc ccaactttga cacgcgcgac cgtgtagatg tggccatcgt
     7381 gggtgcgggc acggccggcc ttgccgccct gcgagaagtg cgcgatcgca ccaacagctt
     7441 cgtcctcatc aacggcggcc catacggcac gacctgcgcc cgcgtcggct gcatgccatc
     7501 caagacgctg atcgccgccg ccaatgcgtt ctacctccgg acgaagctcg atgagttcgg
     7561 cattcgtggc ggggataggc tcgtcgccga catcccggcg gtcctgcatc gtgtccgctc
     7621 gctgcgggat catttcgttg ctggagtgct aaaggcgaca gaggatctcg gcgaccgcaa
     7681 gatttcaggg tacgcacgcc tcgatggccc cggccggttg atcgttaacg accgtgtgat
     7741 ccaggcacgc cagatcatac tggcgccggg atcgagtccg atcgtgccaa agccctggcg
     7801 cgccctgggc agccgcatac tgacgtccga tacgctcttc gagcaggaga atctgccgcc
     7861 ccgggtcggg gtcatcggtc ttggcccgct tggggttgag atcgcacaag ccctggcgcg
     7921 gctcggtctc gaggtctatg gcttcgacgc actcgagcgg gtggctggca tttcggatga
     7981 gaaggtttcg gcggcggcgc tgaactgctt agccagcgaa ttcgccatcc acctcggtgc
     8041 tcaggtcgac ctcgccgaag tggatgacgg ggtcgagata cgatgggacg acaagaatgt
     8101 cgtcgtcgac aaagtcattg tggcgatcgg ccggcgcccg aacctggagg gactcggact
     8161 ggaaacgctg ggcgtgccgc tcgatgagaa gggcatgccc gaggtcgacc cgtcgacaat
     8221 gcggattcct ggcacatcgg tcctgctggc cggcgacgcc aatggggata ggccgctatt
     8281 gcacgaagct gccgatgaag gccacattgc cggcctcaac gcaaccagcg atttaccaat
     8341 ccagctgccc cggcgcacgc cgctgagcat cgtgttctgc gatcccgaga tcgccactgt
     8401 cggacaaaga gcaaaggacc tcgatccgga aaccattctg attggggagg cgagctttgc
     8461 cggccaaagc cgggcgcgaa taatgcagga aaaccgtgga ctgctgcggc tctacgcagc
     8521 gcgccagggc gggcgtttgc tgggcgccga aatggcagtt ccgggggccg agcacatggc
     8581 ccatctcctg gcgttggcga tcggccagga actcaccgtg catgacctgc tgcgcatgcc
     8641 cctttatcat ccgaccctcg aggaaggcct ccgcacggcc ctccgcacaa ttgcgcgtca
     8701 gttgccgccg tgcgcacttt ccgatctcgc ccgctgtggc tcactcgagg tcgaggcgct
     8761 ggagtagagg tatgagcggt cacaagagca aagccgttgg ggtcagatag cgcggactga
     8821 aggcctctgc ctcccccacc ttggaaccac tcggtgcatg gtttcgagat tgaggctgca
     8881 tcggaggcga tgtgtcaccg gggcccagtg aaaccatgct gggcaattcg gccgtggacc
     8941 tcactgtcga catcagggcg ttaacacctg acgagctgcg cgcggcgacg gccctccttg
     9001 cgcacggcat gcgtgataac cctttgcatg tgagggtgtt tggaactgat ccggagcgca
     9061 gaggacggcg cctaattcgc ttccttgatc ctttggttgc ctatgtccac gcgcatggcg
     9121 cagtcctggg tgcgtatgtg aagggcgagc tggtcggtgt actgggcatg atggagccgg
     9181 gcggctgtcg tccagccctg atggatgcac tgcgccttgc aagcatgatc gtggccagca
     9241 acccaccgga cggggcgttg cgcatccttc gctggctggc gacctgggcg cgtaacgacc
     9301 ccgcttggcc gcactggcat atcggtccaa tgacggtgga tcccgcgtac cgacggcgcg
     9361 gcatcggacg gcatttgatg acccattgct gccggcagat ggacgcgctc gcggccacgg
     9421 cctatctgga gaccgataca aaaacaaacg tggcctttta cgaaacgctt ggctttgccg
     9481 tcatcaagca cgaacgcgtc cttggcgtgc cgaactggtt catgagccgt ccgccgtcgc
     9541 accggaagac cgctccgtca gatgcggtcc ccaccagcga aatcccacga tgacctgctg
     9601 ccagccgtct gccgggagtt ggctgggcga cccagaaaac acctggtaga taggggcacc
     9661 cggcccacgc tagccggcga tgcgggcgcg tgaaattgca ctcaccccgc ttgcacgtgc
     9721 gcggggtcca tccagaaggg ctcccatgtg tggccgtcga gatcttcgaa ggtgcgctgg
     9781 accatgaatc catggtcctg cttcggcctg ggctccttgc cgcccgcctc gacggccttg
     9841 gcgaccatct cgtcgacggc ggccttgctc tcgagtgcca gcgcaatcag cacctcggtg
     9901 gatttgtgcg cgtcagcgat cggtttgact gagaatttgt tgaaatacgc gtgcgtcagc
     9961 agcatggcgt agttgtgctc cgaaatgacc atgcaagcgg catcatcgtt cgtgaattgc
    10021 ttgttgaaac tgaagccgag cgcctcaaag aaagccatgg actttttgag atccttcact
    10081 ggcagattga cgaaaatctt cgtgctcatg attgctcctt ggtgagacag gcatgcaggt
    10141 caggcgttcg ggacgatgat catccacgag acgccaaatt tgtcggcaac ggcgccgaag
    10201 cgcttggcga aaaacgtcgg gccgatcggc atctgcactg tgccatcggc cgcaagtgcg
    10261 ttgtagattc ggtcgcattc ggcctcgctc gaaacggcca gcgacagcga catgcactcg
    10321 aagtcgagcg gacccgactt cattccgtcc gacatcatga tgacggtccc agcaatgcgc
    10381 aggcttgcgt gcatgatccg gctgtcgaac tctgccggca ccacgtcaca tccgggctta
    10441 tcgggattat cactgaagcg catcatcatg agcacttcgg caccgagaac gttcttgtag
    10501 tagtcgatcg cctcctcgca ccggccgcgg aagaacaggt acggctggat ccttgggatc
    10561 ggcaactccc caacgaatgg cttgaagccg ccccagaaca tccgtttgcc gtcgaacggc
    10621 gcacttttca tatcgtgcat gcttttcatg cgcgggtcct tgaagacctt cgcattcacc
    10681 tcgtcccgat gctcgcgcga gcgataggtg atgaacgaca cgacgacgaa ttcgtcgtcc
    10741 ttgagatcga cgctctgtgg aaatgaagtc cactggccgg gctgcacatc atcggcctgc
    10801 agctcgacgt aggagagcgc gccgtgatcg cgccagactt gcgccgacag ctcagcgagg
    10861 gccttgtatt cggcggtctt ggcctttggg acgggaacga cgaaaacatc cgtgtagccg
    10921 gtcatcattg ctgcttctcc tgttggggat tttcttgccg ccactcatgc tgtcgcggca
    10981 gcggccgctt caagtgcggc gacatccaac ttcaccattt tcatcatggc ctgcatgacg
    11041 cgcttgccct tggccgggtc gggatcggct aggagctcga tcagccgccg cggcacgatc
    11101 tgccaggaaa gaccgtactt gtcctttagc cagccgcact gcaccggcct gccgccgtcg
    11161 gcggtcagcg ctgcccagta gcggtcgacc tcggcctgat ctgcgcaatc cacggataac
    11221 gaaatcgcgt cgttgaattt ttcgtgcgcc ccgccgttga tggcctggta tctctgcccg
    11281 gcgagcgtga attcgatgaa cagcacgtcg ccttccttgt tgccgggggt gtcggccttg
    11341 gccaagtgca cgcgatcaat tgctgaatcc ggaaaaaccg acgtgtagaa ccgtaccgcc
    11401 tcctcggcat tggcctcgaa ccacaggcat gggatgatct tttgacgtgc cgtcataatc
    11461 tggcctctcc agttttcctg ctctgggtgg gagcgccttc gtgctgaccc gacccaagga
    11521 cggtggaggc tcccagactc cgacacgggt cagaaaattt ttttatactc ctcctctgct
    11581 tgcgcgcccg ctcctcagct gcccgacttc agacctcgat atcgaaatga tgggccgttt
    11641 ccttcgtccc cagggattcg tagagcgcta tcgcag
//
LOCUS       DASVDU010000048         8201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_547192, whole genome shotgun sequence.
ACCESSION   DASVDU010000048 DASVDU010000000
VERSION     DASVDU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547192"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VF226_03325"
     CDS             <1..1341
                     /locus_tag="VF226_03325"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7073047.1"
                     /translation="EAVVAAVLERIAEVNPKINAFTTIAAEQALEAARAADVRRTKGQ
                     ILGPLDSVPVSIKDFIFTRGILTTGGSKVYKDFVPEEDAIVVSRLKAAGAIVIGKTTT
                     PEYCHKTVTDSPLTGVTRNPWNLDLTTGGSSGGSAAAVAAGLGPLSIGTDGGGSIRLP
                     AALCGVAGLKPTAGLVAQGPGFSGWNLVGHTGPLARSVSDLQLVLDVIAGPDVRDPVS
                     LTATRKSFREEPRVAWTATLNDMEPEPNVASQLTRVVDAARRLTTRRVDRVSPRWDDP
                     DLNFRILIGADVASALAPHLDEHRQVMDPSLVKLVEFGQSLSAIDLARALDWRLSFSA
                     NVLSFFENYDLLIVPTSPISAFSHTIIGPTTIAGRKCSPYAWFGWTWPFNVTGQPALS
                     LPVIGDDQMPVGIQIIGRPGEDDLVLSFSRRLDDALGRPPLKPRGIDVLTGSDL"
     gene            1396..2277
                     /locus_tag="VF226_03330"
     CDS             1396..2277
                     /locus_tag="VF226_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7073048.1"
                     /translation="MPFVTTTDGVKLSVEEAGDGTPLLFVHEFAGDQRSWETQVRYFS
                     RRYRCITFNARGYPPSDVPGDIENYSQARAAEDIRDVLDGLNIDRAHVVGLSMGGFSA
                     LHFAIGHPQRVRSLVIAGVGYGAEKHLESVFRDAANQAADQFEKLGSEAFSHTYCAAA
                     ARIPLLLKDPRGWEEFRRMFGEHDSRGAALTMRGVQARRPSIYDLEESLRRMEVPVLI
                     VSGDEDDHCLLPGLFLKRTIPASGLAVLPKTGHTLNLEEPAMFNSLVADFLAQVEQGR
                     WLPRDPRSNPAEIVKTN"
     gene            complement(2392..3309)
                     /locus_tag="VF226_03335"
     CDS             complement(2392..3309)
                     /locus_tag="VF226_03335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7073049.1"
                     /translation="MKAIVVTDQAAGIAGMKLVERPEPQAAINDVVVQVHASGFVPTE
                     LTWPPTWTDRVGRDRTPSIPGHELAGVVTALGYGTRGLSVGQRVFGLTDWTRDGTLAE
                     YTAVEARNLAPLPGDVDFTVGASLPISGLTAWQGLFEHGRLQAGQSVLAHGAAGAVGS
                     MVTQLAREAGAYVIGTGRAADRRTVLDFGAQEFVDLENDVLEDVGEVDLVFDVIGGDI
                     GKRSARMVRAGGTLVSVVGPSDARPADGLAIDFVVESDRAQLGEIVQRVRDGRLRTNI
                     GAVSTLDDALAALNPTERRKGKTIICVRP"
     gene            complement(3456..5657)
                     /locus_tag="VF226_03340"
     CDS             complement(3456..5657)
                     /locus_tag="VF226_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX7073050.1"
                     /translation="MPFKVEVGPPQIAIHHGQTVLITELDAQINWPSDRGLYFNDTRI
                     LSSWQIYANGEPWDLLTGGATTYYTSEVHLTNRAIMTEDGAIPPHTLGLVISRSVEGG
                     MHEDLDVTNNGAKHVRFQLEIAMRSDFADIFEVKAGNIVRRGRITTEWSASKQRLRTT
                     YQNADFVRSLAVSAWHSQSKAVYANGRLSFEVSLAPGEAWHSCLLYTPSDGERDYSSP
                     HHCVSDSDKLDHAQTLQDWLQTVLKISTGNEEFYRLYRQALEDMAALRLPMSTTGTDR
                     MVFMPAAGLPWFVAPFGRDSLIVSLQNLLIYPGFARGALETLGSLQATERDDYRDAEP
                     GKILHELRRGELAHFKLIPHTPYYGTADATPLYLITLHWAWRATGDRELIEKKLETAE
                     RCLAWIDQYGDRDGDGFQEYQTRSPVGYENMGWKDAGDAVVYPDGSLVKGPKALCELQ
                     GYVYDAWLRMAEIFDELGNHVRARELRAKAQQLFEKFNDQFWDEGLGYYAYALDGDKK
                     KVLTIASNPGHCLWSGIVPPERAAKVVQRLMAPDMCCGWGIRTLSTKNPAFNPYNYQN
                     GSIWPHDNAIIAMGFRRYGFGAEAARVARAISEAGSHFLLNQLPELYTSTQHHEPSFP
                     IQYLGANVPQAWAAGAVFMLMQTMLGFMPDAPRGKLYVDPDLPPWLPDLTVMDLRMNQ
                     HKFDIRFWREGDQTKFKVLRGDEKMIEYSPMTVQFNRLKKVTEVETTLQTP"
     gene            complement(5732..6655)
                     /locus_tag="VF226_03345"
     CDS             complement(5732..6655)
                     /locus_tag="VF226_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7073051.1"
                     /translation="MPVTQLIGFSVTVSDLAGAAAFYRDALGLQIGPEQTFRNSAWNR
                     LLGLAPDTTARAVDIAIGRQTVQLVAFDPLGRPYPPERASNDQWFQHFALVSGNISKS
                     WERLIISSSPSEITKGPPVLLPPNTGSVTAFKFRDPEGHPLELISFPPDIGAAVWQET
                     SGEGILGCDHTALSIMDLDRSIAFYSGLLGFRVTGRSLNHGPEQDRLDGLVGCEVDVV
                     ALEPAAVAIPHVELLHYRKPPGRISTLPVQANDVASVRQVHKVDNLGALVEGLHAGGT
                     TFVSPGVVSLEESGKAAAIRDPDGHMILLME"
     gene            complement(6670..7122)
                     /locus_tag="VF226_03350"
     CDS             complement(6670..7122)
                     /locus_tag="VF226_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7073052.1"
                     /translation="MTSEPPKLSYWHVWADDQGVTHQTRCDLTAFTLASVGGKAAPQW
                     NDHLLTGKTNIQFTVLPVGWIGDWHRNPEPQWIAVLSGRWFVETTDGMRTEMRAGDVS
                     FGGDQHAKVDAQGRVGHRSGTLGNEPCALMIVQLNSENWVGARPGSFK"
     gene            complement(7126..7599)
                     /locus_tag="VF226_03355"
     CDS             complement(7126..7599)
                     /locus_tag="VF226_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7073053.1"
                     /translation="MEWLVNDALPVLARIFIVWIFPFSFLDKIINWDSALKQASSSWL
                     PGGPVLLILAMIVELLTPPMIVFGFYDGIAAFILAGYCVVTGIVYHHFWTYPRFWSPR
                     SEGYPHIWDFFKNFGLVGGLIFIMYASGFIQAAEKDLERVSAAHAVTVVLASDRN"
     gene            complement(7618..7935)
                     /gene="cyoD"
                     /locus_tag="VF226_03360"
     CDS             complement(7618..7935)
                     /gene="cyoD"
                     /locus_tag="VF226_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HEX7073054.1"
                     /translation="MDASGLPVERSIRPYLIGLGLAFILTAIPFALVALSALPRFTML
                     IVIAVAAVLQILVHFRFFLHIGIRSTPAENLIALAFAAILIVFMVGGTLWIMFDLHYR
                     MMV"
     gene            complement(7938..>8201)
                     /locus_tag="VF226_03365"
     CDS             complement(7938..>8201)
                     /locus_tag="VF226_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7073055.1"
                     /translation="GEFSGMISQGAGPDRSGFLSAFFTLVGTHGLHVSIGILWIIVMI
                     GQVLAKGMTRPVASRLIRLGLFWHFLDIIWIGIFSVVYLPGVL"
BASE COUNT         1658 a         2491 c         2427 g         1625 t
ORIGIN      
        1 gaggcggtcg tcgctgccgt cctggagcgg attgccgaag tcaatcccaa aatcaacgcg
       61 tttacgacca ttgcagccga gcaggccctc gaggcagccc gcgctgccga cgtccggcgg
      121 acaaagggtc agatcctcgg accgctggac agtgtgccgg tctcgataaa ggacttcatt
      181 tttacacgcg gaattctgac gaccggcggt tcgaaagtat acaaggactt cgtgcccgaa
      241 gaggacgcga tcgtcgtctc gcgattgaag gcggccggcg ccatcgtcat tggcaagaca
      301 accacgcccg agtattgcca caagaccgtc accgacagtc cgctgacggg tgtcacgcgc
      361 aatccctgga atctcgactt gacgactggc gggtcctcag gcggcagcgc ggctgcggtc
      421 gcggcagggc tcggaccgct gagcatcggg accgacggag gcgggtcaat tcgtctgccc
      481 gcagctctct gcggggtggc cgggttgaaa ccgacggccg ggctggttgc gcaggggccg
      541 ggcttcagcg gctggaactt ggtcggccac accggcccgc ttgcgaggtc ggtcagcgac
      601 ctgcaactgg tgctcgacgt catcgcgggg ccggacgttc gcgatccggt atccctcacc
      661 gcaacccgca aatcatttcg cgaagagccg cgcgttgcct ggaccgccac gttgaatgac
      721 atggagcccg agccgaatgt ggcatcacag ttgacacgcg tcgtggacgc ggcgcgccgg
      781 ttgacaaccc ggcgcgttga ccgcgtatcg cctcgctggg acgatcccga tctcaatttc
      841 cggatcttga tcggcgcgga tgtcgcctcg gcgctcgctc ctcatttgga cgagcatcgt
      901 caagtgatgg atccgagcct cgtgaaactg gtggagttcg gtcaatccct ttcggcgatc
      961 gatctggcca gggcgctcga ttggcggctt tctttctcgg ccaatgtcct ttcgttcttc
     1021 gagaattatg acctgctgat cgtcccgacc tcccccataa gcgcattctc gcacacaatc
     1081 atcggcccga cgaccatcgc cggcaggaag tgctcgcctt atgcctggtt cggatggaca
     1141 tggccgttca acgtcactgg acaacctgcc ctgtctttgc cggtgatcgg cgacgatcag
     1201 atgcctgtcg gcatccagat tattgggcgg cctggagaag atgatctggt tttgtctttt
     1261 tcccgccggc tcgatgatgc gcttggccgc ccgcctctaa aaccgcgtgg cattgatgtt
     1321 ttgactggat cagacttgtg aaacaagagc tgggcggcag cctgatgctc catttgatgc
     1381 aaggggagtg aagcgatgcc tttcgtcacg acgactgatg gagtaaagct cagcgtggaa
     1441 gaagctggcg acgggacgcc gctattgttc gtccacgagt tcgccggcga tcagcgcagt
     1501 tgggaaactc aggtccgcta cttttcgcgg cgataccgct gcatcacttt caacgcgcgc
     1561 ggctacccgc cttctgacgt tcctggcgac atcgagaatt actcgcaagc acgcgccgcc
     1621 gaggatatcc gtgatgtcct tgacggcctg aacatcgacc gggcgcacgt ggtcggcctt
     1681 tcgatgggcg gtttctccgc gttgcatttc gcgattgggc atccgcagcg ggtgcggtcg
     1741 ctcgtgatcg ccggtgtggg ctacggtgca gagaagcact tggaaagcgt cttccgtgat
     1801 gccgcaaatc aggccgccga tcaattcgag aagcttggct cggaagcctt ctcccacacc
     1861 tactgcgccg ctgccgcacg tatcccgctg ctgctcaagg atccgcgcgg ctgggaggag
     1921 ttccggcgga tgttcggcga gcacgattcc cgaggtgccg cgctgaccat gcgtggcgtc
     1981 caggcacgcc ggccttcgat ctatgacctc gaggagtcgc tgcgccgcat ggaagttccg
     2041 gttctaatcg tttcgggtga cgaggatgat cactgcttgc taccgggcct gttcttgaag
     2101 cggaccattc ccgcaagcgg tctcgccgtt ctgccgaaga ctgggcacac gctcaatctt
     2161 gaagagccgg ccatgttcaa ttctctggtg gcggacttcc tggcccaggt cgaacaggga
     2221 cgctggctac cgcgcgatcc ccggtccaat ccggcggaaa tcgtgaagac caattgaaag
     2281 gcgagtgcct cgacgagttc tatcgtgtcg cgttccgtaa gggatgatcg ccacggtctg
     2341 gaacgacaac gatgatccgc aagaacggaa ccgatcgccc ttcccgagtt ctcacggacg
     2401 aacgcagatg atcgtcttgc ccttgcgtcg ctcggtcgga ttcaaggcgg cgagggcatc
     2461 gtcgagggtc gagacggcgc cgatgttcgt ccgcaggcgt ccgtcccgca cccgctggac
     2521 gatctcgccc agttgggcgc gatcggactc gacaacgaag tcgatcgcca agccgtcggc
     2581 cggtcgcgca tcagatggtc cgacgacgga caccagcgtt cctcccgctc gaaccatgcg
     2641 cgcggaccgc ttcccgatat cgccgccgat gacatcgaac accaaatcaa cttcgccgac
     2701 atcttccagg acgtcgttct cgagatcgac gaactcctgc gcgccgaagt cgagcaccgt
     2761 ccgacggtcg gcggcgcgtc cggtaccgat gacgtaggcg ccggcctctc gtgccagttg
     2821 cgtcaccatc gaaccgactg cgccggccgc gccatgcgcg aggacgctct ggcccgcctg
     2881 aaggcggccg tgctcgaaca gtccctgcca cgcagtcagg cccgagatcg gcaggctcgc
     2941 gcccaccgtg aagtcgacgt cgcctggcag cggggcgagg ttacgtgcct cgaccgccgt
     3001 gtactccgcc agggtgccgt cgcgagtcca gtccgtaaga ccgaacaccc gctgtcccac
     3061 cgacagccct ctcgtgccat aaccgagcgc cgtgaccact ccggcaagct cgtgcccagg
     3121 gatcgatggt gtccggtcgc ggccgacgcg atcggtccag gtcggcggcc acgtcagctc
     3181 agtcgggacg aatcccgaag cgtggacctg aacaacgacg tcatttatcg ctgcctgcgg
     3241 ctcgggccgc tccaccagct tcatccctgc tattcccgca gcctggtccg tcactacgat
     3301 cgctttcaca ggaatggtct ccattcatcg cgggaatgca tttcgcgtaa tcgtgtatcg
     3361 cgcttagctg atacgagcgc ctgctcgtcg atgctgagca ttgcgaaaat cgcccagtgt
     3421 ccggtctgga cggactcggg cgcgccgtaa tctcgtcaag gtgtttgcag tgtggtctct
     3481 acttccgtta cctttttgag ccgattgaac tgcacggtca tcgggctgta ctcgatcatt
     3541 ttttcatcgc cacgaagcac cttgaatttc gtctggtcgc cttcgcgcca gaaacggatg
     3601 tcgaatttgt gctgattcat acgaaggtcc atcacagtga gatccggtag ccacggtggc
     3661 agatccgggt ccacgtaaag cttgccgcgc ggggcatccg gcatgaagcc aagcatggtc
     3721 tgcatcagca tgaacaccgc gccggcggcc cacgcctgcg gcacgtttgc cccgagatac
     3781 tgaatgggga aagatggctc gtggtgttgc gtgctcgtgt agagttcggg gagttgattg
     3841 agcaggaaat ggcttcctgc ctcgctgatt gcgcgggcga cccgggcggc ctctgcgcca
     3901 aaaccgtaac gcctgaatcc catcgcaatg atggcattgt cgtgcggcca aatcgatcca
     3961 ttctgataat tatacggatt gaatgcgggg ttcttcgtgg acaacgtgcg aataccccac
     4021 ccgcaacaca tatccggtgc catgagcctc tgcacaacct tggcggcccg ctcgggcgga
     4081 acgatgccgg accataggca gtgcccggga ttggaggcga tcgtcaacac cttcttcttg
     4141 tcgccgtcga gcgcataagc gtagtagccg agaccttcat cccagaattg atcattgaat
     4201 ttttcgaaca attgttgggc tttcgcacgc agctcgcgag cgcggacgtg attgccgagc
     4261 tcgtcgaaaa tctcggccat tcgcagccac gcgtcataga cataaccctg cagttcgcat
     4321 agcgccttgg gtcccttcac gagtgagcca tccggataga caacagcgtc cccggcgtcc
     4381 ttccaaccca tgttctcgta accgaccgga gatcgcgtct ggtattcctg gaacccgtcg
     4441 ccgtcgcgat cgccatattg gtcgatccag gcaagacaac gctctgctgt ttccagcttc
     4501 ttctcaatga gctcccggtc tccagtggcg cgccacgccc aatgcagcgt aataagatag
     4561 agcggggtcg catctgccgt cccataatag ggcgtgtgcg ggatcaactt gaaatgggcc
     4621 aattcaccac ggcgcaactc gtgcagaatt ttcccgggct ctgcgtcgcg gtaatcgtct
     4681 cgctcggttg cttgcagcga gcccagtgtt tcgagcgcgc cgcgggcgaa gccgggatag
     4741 atcagcaggt tttggagcga aacgatcaga ctgtctcgac cgaatggggc aacaaaccag
     4801 ggcagacctg ccgcaggcat gaagaccata cgatcggtgc cggtggtact catgggcagg
     4861 cgcagcgccg ccatgtcctc cagcgcttgc cggtaaagac gatagaactc ttcattgccg
     4921 gtgctgatct tcaggactgt ctgcaaccag tcctgaaggg tctgggcgtg atccagcttg
     4981 tcactatccg aaacacagtg atgcggtgag gaataatctc gctcaccatc ggaaggcgtg
     5041 tagaggaggc aactgtgcca ggcttcgccc ggggccaaag aaacttcgaa gctcaggcgc
     5101 ccgttggcgt aaaccgcctt cgactgacta tgccatgctg agaccgccag tgagcgcacg
     5161 aagtcggcat tttggtaagt ggtccgtagc cgctgtttcg acgctgacca ttccgtcgta
     5221 attcgaccgc gtcgtacgat attccccgcc ttgacctcga aaatgtcggc gaagtccgac
     5281 cgcatcgcga tctccagctg gaaccgtaca tgcttcgcgc cattgttcgt gacatccaga
     5341 tcctcatgca tgccgccttc gaccgaccgg ctgatcacga ggccaagcgt atgcggtgga
     5401 atggcgccat cctcggtcat gatggcgcga ttggtgaggt ggacctcgga ggtgtaatag
     5461 gtggtggcgc cgccagtcag aagatcccaa ggctcgccgt tggcatagat ttgccagctg
     5521 ctgaggatcc gtgtgtcgtt gaaatagaga ccacggtcac tcggccagtt gatctgtgca
     5581 tcaagctcgg tgatcagcac tgtctggccg tggtgaattg caatctgcgg cgggcctact
     5641 tcgactttga atggcatggt gtctgaacca ctctgagact aagggaagct tatcgcattt
     5701 tccgtaatta gggatgagcg cgaaatacgc ttcattccat cagcaaaatc atgtgcccat
     5761 ccggatcgcg tatcgccgct gcttttccac tctcctccag gctcaccacg ccgggagaca
     5821 caaacgtcgt acctccagcg tggagcccct cgacgagcgc accgagattg tcaaccttgt
     5881 ggacttggcg gaccgaggca acgtcgttgg cctggaccgg caatgttgag atccggccgg
     5941 gtggcttccg ataatgcaag agttccacat gcggtatggc gacggccgcg ggctcgagcg
     6001 ccaccacgtc aacttcgcat cccacgaggc catcgaggcg atcctgctca gggccatggt
     6061 tcagcgaccg gccagtgacg cggaagccga gtaagccgga ataaaaagca atgctccggt
     6121 ccagatccat aatcgagagc gccgtgtgat cgcagccaag gatgccctcc ccacttgtct
     6181 cctgccaaac agcagcgccg atgtcgggcg gaaaggagat cagctccagc gggtgtccct
     6241 cagggtcgcg gaacttgaag gcggtgacgc tgccggtgtt gggcggcagc agcacaggcg
     6301 ggcctttcgt gatttcactc ggtgagctgc ttattattag ccgttcccag gatttcgaaa
     6361 tgttaccact gacgagagcg aaatgctgga accattgatc atttgaggcg cgttcgggag
     6421 gatatggtcg tcctagagga tcgaacgcca ccaactggac cgtctgccgc ccaatcgcga
     6481 tatcgacagc ccgcgccgtg gtgtctggcg caagtccgag gaggcggttc caagctgagt
     6541 tccggaacgt ctgctcaggc ccaatctgca ggccgagcgc gtctcggtaa aaggcggctg
     6601 ctcccgccaa atccgagact gtaacgctga acccgatgag ttgtgttacc ggcattccgc
     6661 cacgcaatcc tatttgaatg aaccgggtct ggcgccgacc caattttctg aattgagctg
     6721 aacgatcatg agcgcacagg gctcgttgcc gagcgtaccg gaacgatggc ctacacggcc
     6781 ctgggcatcc accttcgcgt gctgatcgcc gccgaaagaa acatccccag cccgcatttc
     6841 ggtgcgcatt ccgtcggtcg tctcgacgaa ccagcgcccc gaaaggacag caatccactg
     6901 gggttccgga ttccggtgcc aatcgccgat ccagccaact ggcagaaccg tgaactggat
     6961 gttggtcttt ccggtcagca gatggtcatt ccattgaggc gctgctttgc ctcccacact
     7021 agcaagcgta aaggccgtca ggtcgcagcg cgtctggtgg gtcacaccct gatcgtcggc
     7081 ccacacgtgc caataggaaa gtttcggtgg ctcgctggtc atgtatcagt tccgatcgct
     7141 ggcaagaacc accgtcacgg cgtgcgcggc ggagaccctc tccaaatcct tctccgcagc
     7201 ctgaatgaac ccgctcgcgt acatgatgaa gatcaatccg cccacaaggc cgaaattctt
     7261 gaagaagtcc cagatatgcg ggtaaccctc gcttcgaggt gaccagaagc gcggataagt
     7321 ccagaaatga tgatagacga tgcccgtgac cacgcaatat cccgcgagga tgaaggccgc
     7381 gatcccatcg taaaaaccga acacgatcat cggcggcgtg agcagctcca cgatcatcgc
     7441 caggatcaaa aggaccggcc cacccggaag ccagctcgag ctggcctgtt tgagcgcgct
     7501 gtcccagttg atgatcttgt ccaggaagct gaacgggaaa atccagacga taaaaattcg
     7561 cgccagtaca gggagagcgt cattgaccag ccattccatc gccagcgctc ctttccctca
     7621 caccatcatg cggtaatgca ggtcgaacat gatccaaagc gtcccgccga ccatgaagac
     7681 gatcagtatc gcggcaaacg ccagggcgat gaggttctct gccggcgtgg agcggatacc
     7741 gatgtggaga aagaatctga aatgaaccag gatctggagg acagccgcca cggcaatgac
     7801 gatgagcatg gtgaatcgcg gcagagcgct taaagcgacg agtgcgaaag gaatcgcagt
     7861 caatatgaag gcgagcccta gtccaatgag atagggcctg atactgcgct cgactggcag
     7921 acctgaggca tccatttcta aagcactcct ggcagataaa cgaccgagaa tatgccgatc
     7981 cagataatgt ccaggaagtg ccagaagagg ccgagccgga tgagccgcga ggcaaccggc
     8041 ctcgtcatgc ccttggccaa aacctggccg atcatcacta taatccagag aataccgatg
     8101 ctcacgtgca gcccatgcgt gccgaccagc gtaaagaagg cagagagaaa cccgctgcgg
     8161 tcggggcctg cgccctgcga gatcatacca ctgaactctc c
//
LOCUS       DASVDU010000049        16213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1482731, whole genome shotgun sequence.
ACCESSION   DASVDU010000049 DASVDU010000000
VERSION     DASVDU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482731"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /locus_tag="VF226_03370"
     CDS             complement(<1..1291)
                     /locus_tag="VF226_03370"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7073056.1"
                     /translation="MAGADLLAILPFLVLAAAIVVVMLAIAIRRHHDLTFGLALIGTL
                     LSFVSLWPASEVAPRRVTLLFIIDGYALFYIGLILATTLSVLLFSHGYLKMRRGPQEE
                     FYLLVLLATLGAISLVASCHFASFLVALETLSIALLALIAYPRSRARPVEASLKYLIL
                     AGTSSAILLFGMALIYARLGTMEFSLIGDRLRTIAEASDIYWLTGLAMIVAGIGFKLS
                     LVPFHMWAPDVYEGAPAPVTAFIATVSKGAVFALLLRYFLNADAYNFGSVFIMINVLA
                     VASMLAGNLLALLQNNVKRILAYSSIAHLGYLLVAFLAGGALAKEAVTYYLVAYFVMT
                     LGAFGIVIVLSSVAGETEVEELADYRGLLWQRPWLGGVFTITLLALAGIPLTVGFFAK
                     FYAVAGGVGAAAWPAIFALVAGSVIGLFYYLRVIVIMA"
     gene            complement(1295..2794)
                     /gene="nuoM"
                     /locus_tag="VF226_03375"
     CDS             complement(1295..2794)
                     /gene="nuoM"
                     /locus_tag="VF226_03375"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7073057.1"
                     /translation="MMLLWIFLVLLIGGALAWLAQRGGRDWPRWVSLIVLVVDLVLVL
                     MVWSENYEVASVASRGTWFAELDLPWIPMLGIRFHLGLDGLSLLLILLTIGLGIMAVA
                     ASWSEITDRVGFFHFNLLWTLAGTVGVFLALDLFLFFFFWELMLFPMYFLIAIWGHER
                     RVYAAIKFFLFTQGSGLLMFVAILALVFINHQQTGATTFDYFALSGTELDASTEMLIM
                     LGFFIAFAVKLPAVPLHTWLPDAHTEAPTAGSVILAGILLKTGAYGLLRFAVPLFPDA
                     AMSFAPVAMALGVIGTLYGAVLAFAQDDMKRLVAYTSVSHLGFVLLGIYAWNELALQG
                     AVVQMLAHGLSTGGLFILVGALQERLHTRDMRQMGGLWARMPRLSAIGMFFAIASLGL
                     PGLGNFVGEFLILLGAFSRSAGFAIAGAVGLVASAIYALILVQRAFHGEPRGAAALPD
                     FSVRHMTIMAVMIATLIWLGFYPQPVIDTFRPGLGNLHQLATGQAEAAR"
     gene            complement(2791..4683)
                     /gene="nuoL"
                     /locus_tag="VF226_03380"
     CDS             complement(2791..4683)
                     /gene="nuoL"
                     /locus_tag="VF226_03380"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7073058.1"
                     /translation="MLDLLALVPAFPLFGFAILFITAGTLPRRWIAAIGVGSVGISAI
                     LALIVAAGFLSAQQPYTQIVWRWMAIGGFNSAVGFYLDALSVVMMLVVTFVSFLIHLY
                     SAEFMADDEGYSRYFAYMNLFVAAMLVLVLADNLLLLYLGWEGVGLCSYLLIGFWYRD
                     PANGLAARKAFVVTRVGDTAMVIGLFVLFTHLGALEIQPLMQRAQESWPVGSGLAIAA
                     AGLLLGGALGKSAQLPLQVWLPDAMAGPTPVSALIHAATMVTAGVYLIARTHVLYELA
                     PPIHTAVAVIGALTLLLAAFSALNQTDLKRILAYSTISQIGYMFLALGVGAWAAAIFH
                     FMTHAFFKALLFLAAGVVIQRLHEEHDIFRMGGLRRRLPLAFWSFLIGSASLAALPFI
                     DAGFYSKDLILWETWNSGANGPWLWLAGIVGAFLTAIYIFRAVFVVFFGPVNIEPEGR
                     TGFAIAVPLIVLSLLALAGGFVEIPEYLGGIPAFTELLSAVLPNPPSLTAPTASSALA
                     GVLTGIAALLAMLGVAVAFVAFLDRPAFVTRLIESPVGVTLQRFWAAGWGFDWLYDRA
                     LTRPFVWLAQLNRSDIIDFFYAGVAWLSEAGWRLLSRSQTGLVRWYAAGVAFGSATLI
                     ILAVFA"
     gene            complement(4686..4997)
                     /gene="nuoK"
                     /locus_tag="VF226_03385"
     CDS             complement(4686..4997)
                     /gene="nuoK"
                     /locus_tag="VF226_03385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7073059.1"
                     /translation="MTADVPMQYGLLLAAVLFSLGVIGVLARRNIIFILMSIEIMLNA
                     AGLAFIAAGARWAAADGQIMFILILALAAAEVSVGLALILQFYHRTVTVDADAASEMR
                     G"
     gene            complement(5056..5604)
                     /gene="nuoJ"
                     /locus_tag="VF226_03390"
     CDS             complement(5056..5604)
                     /gene="nuoJ"
                     /locus_tag="VF226_03390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7073060.1"
                     /translation="MTAAFYIAAVVAVLATIMVIIETNAVHALLNLIVSLLAVAIVFY
                     VLGAPFAAALEVIVYAGAIMVLFVFVIMMLNLGEAAVAQERRWLAPRMWMAPAGLSAV
                     LLGELIYMLWQGPAAVPVGTTVTPREVGALFFGPYLLVVELASMLLLAGLVGAYHLGR
                     RERAVAKPEMETAREAQQETQR"
     gene            complement(5601..6116)
                     /gene="nuoI"
                     /locus_tag="VF226_03395"
     CDS             complement(5601..6116)
                     /gene="nuoI"
                     /locus_tag="VF226_03395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX7073061.1"
                     /translation="MLSQLRSIWYVFLHLFRRRVTVQYPEEKPYLPPRFRGRIILSRD
                     PDGAERCVACYLCAVACPVDCISLQKTEDETGRWYPEYFRINFSRCIFCGWCEEACPT
                     YAIQLTPDFEMSEYERHNLVYEKEDLLISGTGKYPGYNFYRVAGRAVGGKDKGQAENE
                     EPPVDLRGLMP"
     gene            complement(6129..7088)
                     /gene="nuoH"
                     /locus_tag="VF226_03400"
     CDS             complement(6129..7088)
                     /gene="nuoH"
                     /locus_tag="VF226_03400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018446414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7073062.1"
                     /translation="MTELAQGIANVFGILIVLVAVAAVLTWIERRLLGLWQDRYGPNR
                     VGPFGTLQVVADMIKLLFKQDWIPPFADRLVFVLAPAIIMATVLLAFAVIPIAPGIGI
                     TDLGNIGILFFLAMSSLSVYSAVLAGWASNSKYALLGSIRAAAQMLSYEVFMGLSFLG
                     VVLLAGSFNLREIVEAQRGLWHIGPQFVGFVIFLIAGIAETRRLPFDLPEAESELVAG
                     YHTEYSSLKFGMFFVGEYVGILLISAMITTLFFGGWLGPVLPPILWFLIKTGFFVCFF
                     ILLRAALPRPRYDQLMALGWKAMLPLSLLNILATGAVLLAIGH"
     gene            complement(7081..9816)
                     /gene="nuoG"
                     /locus_tag="VF226_03405"
     CDS             complement(7081..9816)
                     /gene="nuoG"
                     /locus_tag="VF226_03405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX7073063.1"
                     /translation="MVRIEIDGKSYEVETGQNLLHACLSLGFDIPYFCWHPAMGSVGA
                     CRQCAVKQFHGADDQHGRIVMSCMTAAADGLRISIADPEVKAFHASIIEWLMTNHPHD
                     CPVCEEGGECHLQDMTLMTGHVYRRYRFRKRTYRNQYLGPFLTHEMNRCIACYRCVRF
                     YRDYAGGRDFDVFGIHRNVYFGRYEDGILENEFSGNLAEVCPTGVFDDKTLSRVYNRK
                     WDLRAAPSICIHCSLGCNTTVNERAGELKRVLNRYNSAVNGYFLCDRGRFGYGFTNSE
                     LRVREPLLRKDGTLSPMTKQAALQRVAEMIGTGKAIGIGSPRASLEANFALRTLVGAD
                     RFYLGLSKSDHELLMLTLAILRNGPARAASLKEAEEADAVLVLGEDVPNTAPRLALSL
                     RQAVRQKSLAIADRLNIPRWQDQAVREAGGNEKSPIFLATPVASRLDDVATRCFRAAP
                     DDLARLGFAVARMIDTAAPAAPELAPEIDGLAREIAEALITAERPLIVAGNGCANAAL
                     IEAAANVAWALCRTGRPGALCLVPPESNSFGLGLMGGGTLAEAFQAIETGEAQTLIIL
                     ENDLYRRTDKAVVDRCLKRARHVIVIDHVMNDTVRRAEIVLPAGTFAETDGTLVSNEG
                     RAQRFFQVVVPGGDVQASWRWMHDLMSILGRPPEPTWRRLDDVIAACAATFPHLAPIR
                     DAAPGADYRVSGSKIPREPHRYSGRTAIHAAETVHEPKPPDDLDSPFSFTMEGYYGGR
                     LPSAVIPYFWAPSWNSVQSVNKFQEEIGGALRGGDAGVRLIEPQAGAAPAYYQRTPPA
                     FTARRGEWLVLPLYEIYGSEELSRLAPAVAERVPAAYVALNAADASSLGTAEGNSVTL
                     HIAGTKRHLPVKIRHDLPKGVAGIPVGLLNVAPLPDWGKITTAGDQDGRQQHRHD"
     gene            complement(9820..11094)
                     /gene="nuoF"
                     /locus_tag="VF226_03410"
     CDS             complement(9820..11094)
                     /gene="nuoF"
                     /locus_tag="VF226_03410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7073064.1"
                     /translation="MERPLTRNIRPGQEPLSIGDYERAGGYSAVRKALRSMMPDEVMH
                     EVTESKLRGRGGAGFPTGQKWSFVPRGEDAPSPKYLVVNADEMEPGTFKDRLLMEGDP
                     HQLIESVILSAFAIEARIAYIFLRAEYVLSARHLRRAIAEASEKGYLGTNILGSGFDL
                     ELHLHASAGRYICGEETALLNGLEGRRAIPRAKPPFPQASGLWGKPTIVNNVETLCNV
                     PHIVNHGAAWFRGLSRSEDGGTKLYGVSGRVKRPGIWELPMGTAAREILEEHAGGMRE
                     GYSFRALLPGGASTEFLLEEHLDVPMDFSAVQKVGSRLGTGTMIILDDRTCPVGMLHN
                     LEHFFAQESCGWCTPCRDGLPWVERMLAAIETGQGQAQDLDILAMHTGFLGPGKTFCA
                     LAPGAMEPLQSGLKYFRADFERHIDERRCPWS"
     gene            complement(11112..11576)
                     /gene="nuoE"
                     /locus_tag="VF226_03415"
     CDS             complement(11112..11576)
                     /gene="nuoE"
                     /locus_tag="VF226_03415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7073065.1"
                     /translation="MLTTAETEAIEAIARRYENKQGASIEALREVQRSRGWVSDECLR
                     DVARLLDMSADELDAVATFYNLIFRRPVGRHVILLCDSVSCWIMGCEALRRGIESRLD
                     IKAGETTADGRFTLLPTVCLGACDHAPVMMVDDDLIGDLEIGQLDEVLARFR"
     gene            complement(11587..13353)
                     /gene="nuoC"
                     /locus_tag="VF226_03420"
     CDS             complement(11587..13353)
                     /gene="nuoC"
                     /locus_tag="VF226_03420"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HEX7073066.1"
                     /translation="MADDPDLQHSSAVLRDLTARFGVEAIRPQETRDSVPTFWTPAER
                     AHEILAYLKEKIERPYRTLFDLTAIDERHRSHRRPGPTGDFTIVYHLLSYDRADDIRI
                     KVALSGDTPTCPTVTDLWRSADWYECEVWDMFGIVFEGHPHLRRILMPPTWQGHPLRK
                     DHPARATEMGIFELPESKQDSEQDALRFRPEDWGMKRRSETSDFMFLNLGPNHPSVHG
                     VFRVALQLDGEDIIDAVPDIGYHHRGAEKMGERQSWHTYIPYTDRVDYLGGVMNNLPY
                     VLAVEQLAGIEVPDRVKVIRIMLAELFRIASHLVFYGTMAQDVGALSPVFYMFTDRER
                     VFEIIEAICGFRMHPAWFRIGGVAQDLPEGWDRLVREFVAYLPPRLDEYDKIVMKNRI
                     FKMRTQGVGAYSLDEAIEWGATGPSLRACGLEWDLRKVRPYSGYEHFDFEIPTGLHGD
                     CYDRTAVRVEEMRQSLRIIRQCVENMPAGPYKAAHPLTTPPLKEHTMHDIETLITHFL
                     NVSWGPVIPPGEASACVEATKGLNSYYLISDGGTMSYRTRIRTPSFPHLQMIPLMCRQ
                     AMIADLIAILGSIDFVMADVDR"
     gene            complement(13358..13996)
                     /locus_tag="VF226_03425"
     CDS             complement(13358..13996)
                     /locus_tag="VF226_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX7073067.1"
                     /translation="MTWSLSRPPATGPNGSTSSSFEEAVRRSVILTRLQDIVSWGQKN
                     SIWPFNFGLSCCYVEMATAFTSKYDIARFGAEVLRASPREADLIVISGTVFIKMAPVI
                     KRLYDQMLEPRWVISMGSCANSGGMYDIYSVVQGVDTFLPVDVYVPGCPPRPDALLEG
                     LVLLQEQIGTERRPLSWVVGPQGVERPPRPSMRDIKRAERSQVTVLRTPDSV"
     gene            complement(13993..14403)
                     /gene="ndhC"
                     /locus_tag="VF226_03430"
     CDS             complement(13993..14403)
                     /gene="ndhC"
                     /locus_tag="VF226_03430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7073068.1"
                     /translation="MTGIPMPLWPLVLYSAAVIGLIAVMLGSSYLLGSRHESKATVQP
                     FESGIVSVGSAHLRFPAKFYLVAMLFVIFDLETVFIFTWAIAFRDVGWAGYIEVLIFI
                     GVLVAALVYLWRCGALDWGPRRERLIPLDRELRR"
     gene            complement(14640..16151)
                     /locus_tag="VF226_03435"
     CDS             complement(14640..16151)
                     /locus_tag="VF226_03435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX7073069.1"
                     /translation="MMTVRERDHTNPRGLILVALMCTMMLAAMDTTIVATAVPQIVAD
                     LGGFRLFSWVFSIYLLAQTVTIPTCGKLSDLFGRKPVLIIGTLVFLAGSAASSLSWNM
                     VSLIVFRGIQGLGAGAIIATVSTLAGDLYSVRERAAVQGWLSSVWGIAAVLGPVLGGA
                     LVEYVSWRWIFLVNLPVGALALALIGAFLHEDFERRHPRIDYLGSVLVLTSVGVVIFG
                     LLQGGQAWAWSSPESIAVFAIAVVLLAGMVWSERRAAEPVLPGWLWRRRILAGSNLAV
                     VGMGLVMMAPMTYLTTFLQSVQGLGPIAAGMVLATMSVGWPTASALSGRLYLRIGFRD
                     TALIGSILILLGSGAFLLMPSPQPVWAVVIDQITLGAGFGLLSTPLLVGVQSVIGWNQ
                     RGVVTGANMFSRYLGQSLGAALFGAVFNATVAGRLANAPVSVADELPRNVNDVIGALH
                     HADIGAAAETYLRQSIDVATRDLFLVMVVVGLLILLILLLVPRHFSIHSSQSK"
BASE COUNT         3699 a         4964 c         4830 g         2720 t
ORIGIN      
        1 tggccatgat gacgatgacg cgcagataat agaaaaggcc gatgacactc cccgcgacaa
       61 gggcgaagat tgcgggccac gccgcggcac ccactccgcc ggcgacggca tagaacttgg
      121 cgaagaagcc gaccgtcagc ggtatcccgg caagtgccag cagcgttata gtgaaaacgc
      181 caccgagcca aggtcgctgc caaagcaaac cccgataatc ggcgagctct tcgacctcgg
      241 tttcaccagc gacagacgac agaacgatta cgattccgaa cgcgcccaaa gtcatcacga
      301 aataggcgac gagataatag gtcacagcct cttttgccaa cgcgccgccg gcaaggaatg
      361 ccaccagcaa atatccgagg tgcgcaatgg aggagtaagc gaggatgcgt ttgacgttgt
      421 tttgcagcag ggccagcaga ttaccagcca gcatggaagc gacggccaga acattgatca
      481 tgatgaacac cgatccaaaa ttataggcat cggcgttgag gaaatagcgc agaagcaggg
      541 caaagacggc gcctttggaa acggttgcga taaaggcggt caccggcgcc ggggcgccct
      601 catagacatc cggtgcccac atatggaagg gcaccaggga cagtttgaag ccgatgcccg
      661 ctacgatcat ggcgaggcct gtcagccaat agatatccga agcctcggcg atcgtacgca
      721 ggcgatcgcc gatcaagctg aattccattg tgcccaggcg ggcgtagatg agcgccattc
      781 caaaaagcag aattgcagag gacgtgccgg ccagtatcag gtacttcagg ctcgcttcga
      841 ccggccttgc gcgactgcgg ggataagcga tgagtgcgag gagggcaatg ctgagcgtct
      901 ccaaggcaac gagaaacgat gcaaaatggc agcttgcgac aagcgagatg gcgccgagcg
      961 tcgccagcag cacgaggaga tagaactctt cctgcggtcc gcgccgcatt ttgagatagc
     1021 catgggaaaa gaggagcacg gaaagagtag tcgcaaggat aaggccaatg tagaagagcg
     1081 cgtagccatc gatgatgaag agcagcgtca cgcggcgcgg cgccacttcg ctagccggcc
     1141 aaagtgagac gaatgacaat aaggtgccga tcaacgccag accgaaggtc aggtcatggt
     1201 gccggcggat cgcgatcgcc agcatgacca cgacgatcgc tgcggcgaga accaggaaag
     1261 gcaggatcgc gaggaggtcc gctccggcca tggctcaccg tgccgcttcc gcttgcccgg
     1321 tggcgagctg gtgcaggttg cccagccctg ggcggaaggt atcgatgacc ggctgcggat
     1381 agaagccgag ccagatcagg gtcgcaatca tcaccgccat gatcgtcata tgccgcacgg
     1441 aaaagtcggg cagcgcagcg gcccctcgcg gctcgccatg gaatgcccgc tggaccagaa
     1501 tgagcgcgta gattgcggat gccaccagcc cgaccgctcc cgcaatcgcg aagccggcag
     1561 accggctgaa ggcgccgagg aggatcagaa attcgccgac gaaattgccg agcccgggca
     1621 agccgagcga agcgatggcg aagaacatgc cgatggcgga aaggcgcggc atgcgcgccc
     1681 ataacccacc catctgccgc atgtcccgcg tatgcagccg ttcctggagc gcgccgacca
     1741 ggatgaacag gccgccggtg ctcagtccat gcgccagcat ctggaccacg gcgccctgca
     1801 gggcgagctc gttccaggcg tagatgccga gcaggacgaa gccgagatgg ctgacgctcg
     1861 tgtaagcgac caagcgcttc atgtcgtcct gggcgaatgc taggacggcg ccgtaaagag
     1921 tgccgatcac tcccagcgcc atcgcaaccg gcgcaaagct cattgcggcg tcgggaaaaa
     1981 gcggaacggc aaagcgcagc aggccgtagg ctccggtttt cagcagaatg ccggcgagga
     2041 ttacgctgcc cgctgtaggg gcctctgtat gcgcgtcggg cagccaggtg tggagcggca
     2101 cggccggcag cttcacagcg aaggcgatga agaagccgag catgatcaac atttcggtcg
     2161 acgcatcgag ctcggtcccg gacagcgcga aataatcgaa ggtggtcgcc cccgtttgct
     2221 gatgattgat gaaaaccagc gccaggatgg cgacgaacat caataagccg ctgccttgtg
     2281 tgaagagaaa aaacttgatc gcagcgtaga cccgccgttc gtgcccccag atggcgatga
     2341 ggaaatacat cgggaacagc atcagctccc agaaaaagaa gaacaggaac aaatcgagtg
     2401 ccaaaaagac gccgaccgtg ccggcaagtg tccacagcag gttgaaatgg aagaagccga
     2461 cgcggtcagt gatctcgctc cacgatgcgg ccaccgccat gatgccgagg ccgatagtga
     2521 gcaggatcag cagcaaactc agaccgtcga ggccgagatg aaagcggatg ccaagcatcg
     2581 gtatccaggg gaggtcaagc tcggcaaacc aggtcccacg agacgcaacg gaagcgacct
     2641 cgtagttttc cgaccagacc atcaaaacca ggacgagatc gaccacgagc acgatgagcg
     2701 acacccagcg cggccaatcc cgtccgcccc tttgtgcgag ccaagctaag gcgccgccga
     2761 tcagaagcac gagaaagatc cacagcagca tcatgcgaac accgcgagga tgatgagcgt
     2821 agcggagcca aacgcgacgc cggccgcata ccagcgcacc aaaccggtct gtgatcggct
     2881 gagtagccgc cacccggcct cgctcagcca agcgacgccc gcatagaaga aatcaatgat
     2941 atccgaccga ttaagctggg ccagccacac gaaggggcgt gtcagggcgc ggtcatagag
     3001 ccaatcgaag ccccagccgg cagcccagaa gcgttgcagc gtcacgccca ccggcgattc
     3061 gataagccgc gtcacgaaag ccggccggtc gaggaatgcg acaaaagcca cggcaacgcc
     3121 cagcatggcc aaaagcgcgg caattccggt gagcacgccg gcaagagccg atgaagcggt
     3181 tggcgcagtc agcgatggcg gattcggaag taccgccgac agcaactctg taaacgctgg
     3241 aatgccaccg agatactcgg gaatttccac aaagccaccg gccaatgcca acagagagag
     3301 gacgatcagc ggaacggcaa tcgcaaagcc ggtccgccct tcgggctcga tattcaccgg
     3361 cccgaaaaag acgacaaata cggcacggaa gatgtagatc gcggtcagga acgcgccgac
     3421 gatgccggct agccacaacc acggaccatt ggcgccgctg ttccaggtct cccacagaat
     3481 gagatccttg ctatagaagc ctgcatcaat gaagggcaga gcggcgagcg acgcgctgcc
     3541 gatgaggaaa ctccagaagg cgagcggcag ccgccgccgc aagccaccca tcctgaagat
     3601 gtcatgctct tcgtgaaggc gctggatcac cacgcctgcg gccagaaaca gcagcgcctt
     3661 gaaaaaagca tgcgtcatga aatgaaaaat cgctgccgcc caggcgccca ctccgagcgc
     3721 gaggaacatg tagccgatct ggctgatggt cgaataagcg agaatgcgct tcagatcggt
     3781 ctggttgagg gcgctgaaag cggccagcag cagcgtcagc gcgccgatga ccgcgacggc
     3841 ggtatggatc ggcggggcca actcgtatag aacgtgcgta cgggcgatca gataaacgcc
     3901 ggccgtcacc atcgttgccg cgtgaatcag agcactgacc ggcgtcgggc cggccattgc
     3961 atcgggcagc cagacctgga gcggcagttg cgccgattta cccaacgcgc caccaagcag
     4021 caatcccgcc gcagcgatcg caagacctga gcccaccggc cagctctctt gtgcgcgctg
     4081 catcagcggt tgaatctcga gcgcgccaag atgcgtgaag agcacgaaca ggccaatgac
     4141 catcgctgtg tcgccgacgc gcgtaaccac aaaagccttt cgcgcagcaa gaccattggc
     4201 gggatcccga taccagaagc caatcaggag atagctacag agccccacgc cctcccagcc
     4261 caggtaaagg aggagcaggt tgtccgcgag gaccagcacc agcatcgcgg cgacgaacag
     4321 attcatgtag gcgaaatagc ggctgtagcc ttcgtcgtcg gccatgaact ccgcagagta
     4381 gagatggatc aggaaactca cgaaggtgac gaccagcatc atcacgaccg acagtgcgtc
     4441 gagatagaag ccgacggccg aattgaagcc tccgatcgcc atccatcgcc aaacaatttg
     4501 ggtgtaaggc tgctgcgcac tcagaaaacc cgccgcgacg atcaaagcca aaatggccga
     4561 gattccgacc gagccgacgc caatcgctgc aatccatcgg cgcgggaggg tgcccgccgt
     4621 gatgaataga atggcgaagc cgaaaagtgg aaacgcagga acgagcgcga gcagatccag
     4681 catcttcagc cccgcatctc gcttgccgcg tcggcgtcga cggtcaccgt gcgatgatag
     4741 aattgcagga tgagggcgag cccgaccgat acctcggcag cggcgagcgc cagaataagg
     4801 atgaacatga tctgaccgtc ggccgctgcc catcgagcac cggcggcaat gaaggcgagc
     4861 cccgccgcgt tcagcatgat ttcgatcgac atcaatatga agatgatgtt gcggcgagcg
     4921 aggacaccga tgacgcccag tgagaacaaa acggcagcga gcagcaggcc gtattgcatc
     4981 ggaacgtcag cggtcatttc tgcaaccact attccttcgt tcaccgactc gctccgtttc
     5041 gcaccctgga ggcattcatc gctgagtttc ctgctgcgct tctcgcgccg tttccatctc
     5101 gggtttggcg acagcccttt ctcgccgccc gaggtggtac gcaccgacga ggccagcgag
     5161 cagaagcatg gacgcaagct cgacaaccaa cagatatggc ccgaaaaaca gcgccccgac
     5221 ttcgcgtgga gtgactgtgg tgccgacggg aaccgcggca ggtccctgcc aaagcatgta
     5281 gatcagctcg cccaggagaa ccgccgacaa ccctgcaggt gccatccaca ttcgtggcgc
     5341 gagccagcgt cgttcctgcg ccaccgccgc ttctcccaga ttgagcatca tgatgacgaa
     5401 aacgaagagc accatgatcg ccccggcata aacgatgacc tcgagggccg cggcgaaagg
     5461 tgctccgagg acatagaaga cgatcgcgac cgcgagtagc gagacgataa ggttgagcag
     5521 cgcatggacg gcattcgttt caatgatcac cattatggtc gcgaggacgg cgacaacagc
     5581 cgcgatgtaa aaggcagcgg tcatggcatc aaccctcgca gatccaccgg cggctcttcg
     5641 ttctcggcct ggcccttatc cttgccgccg accgccctgc ccgcgacgcg atagaaattg
     5701 tagcccggat actttccggt gccgctgatc aacaggtctt ctttctcgta gacaaggttg
     5761 tgccgctcat attcactcat ctcgaaatcg ggcgtgagct ggattgcata ggtcggacac
     5821 gcttcttcgc accagccgca aaagatgcag cgggagaagt tgatgcgaaa atattcggga
     5881 taccagcgcc cggtctcgtc ctcggtcttc tgcagggaaa tgcaatcgac gggacacgcg
     5941 acggcgcaca gatagcaggc gacgcagcgc tctgcgccgt cgggatcgcg tgacaggata
     6001 atccggccac gaaagcgcgg cggcagatat ggcttctctt ccggatattg caccgtgacg
     6061 cgacgccgga acaaatgaag gaagacatac cagatcgacc gtaactgact gagcatgccg
     6121 tcctccgatc aatgtccgat cgcaagcaga accgcgccgg tcgccaaaat gttcagcagt
     6181 gaaagcggca gcatcgcctt ccaacccaag gccatgagct gatcgtagcg cggccgcggc
     6241 agggcagccc gcaacagaat gaagaagcag acgaagaatc cggtctttat caagaaccac
     6301 aggatcggcg gcaagaccgg cccgagccag ccgccgaaaa acagcgtggt gatcatagcc
     6361 gaaatgagga ggatgccgac atactcgcca acgaagaaca tgccgaattt caggctggag
     6421 tattcggtat ggtatccggc aaccagctcg ctttccgcct ccggaagatc gaagggcaag
     6481 cggcgcgtct cagcgatccc ggcaatcagg aagattacga agccgacgaa ctgtggcccg
     6541 atgtgccaaa ggccacgctg cgcctcgacg atctcccgaa gattgaaaga accggccaaa
     6601 agaacgacgc cgaggaacga taggcccatg aacacttcgt agctcagcat ctgtgcggcc
     6661 gcacgaatgc tgccgagcag ggcgtacttg ctattggagg cccagccggc aaggacggcg
     6721 ctgtagacgg aaagtgaact catcgccagg aaaaacagga taccgatgtt gccgagatcg
     6781 gtgatgccga tacccggagc gatcggaatt acggcaaatg ccaggagcac tgtggccatg
     6841 atgatggccg gtgccaggac gaagaccagc cggtcggcaa atggcggaat ccaatcctgc
     6901 ttgaatagga gcttgatcat gtcggccacc acctgcaggg tgccgaacgg accaacacgg
     6961 ttgggcccat accggtcctg ccacagtccc agcagccgcc gctcgatcca ggtcagcacc
     7021 gccgcaactg cgaccaaaac gatgagaatt ccaaagacat tggcgatgcc ctgcgcgagc
     7081 tcagtcatga cggtgctgct gcctcccgtc ctgatcaccc gctgtggtga ttttgcccca
     7141 atccggcagt ggtgcgacat tgagcagccc gacgggtatc ccggccacgc ccttgggcaa
     7201 atcgtgtcga attttgaccg gtaaatgccg tttcgtccct gcgatatgga gcgtgacgct
     7261 atttccctcc gcggtgccga gtgacgaagc gtcagccgcg ttcagggcga cataggctgc
     7321 cggcacacgt tccgccacgg cgggcgccaa tcggctcaat tcttcactgc cgtagatttc
     7381 gtatagcggc agaactaacc actcacctct gcgggcggtg aaggccggag gcgtcctctg
     7441 gtaataggcc ggtgctgcac cagcttgcgg ctcgatcagc cgcacgcccg cgtccccacc
     7501 acgcaaggcg cccccgatct cctcttgaaa tttgttgacc gactgcaccg aattccacga
     7561 cggggcccag aaatagggga ttaccgcaga tggcaaccgg ccaccataat acccttccat
     7621 cgtgaacgag aacggcgagt ccagatcgtc tggcggcttt ggctcgtgca cggtctcggc
     7681 agcgtgaatc gccgtgcgcc cactgtaacg gtgcggttcg cgaggaattt tgctgccgct
     7741 cacccggtaa tccgcgccgg gggctgcgtc gcggatcggt gcgagatgcg gaaaagtcgc
     7801 cgcgcatgcc gcgatcacgt catccaaacg gcgccatgtc ggctccggcg gacggccaag
     7861 aatgctcatg agatcgtgca tccatcgcca actcgcctgc acatcgccgc ctggaacgac
     7921 aacctggaaa aagcgctgcg ctcgaccttc attgctgacg agggtgccgt cggtctcagc
     7981 gaaggttccg gccgggagta caatctctgc ccggcggacc gtatcgttca tcacatggtc
     8041 gataacgatg acgtggcgag cgcgtttgag acagcggtcc accaccgctt tgtcggtgcg
     8101 gcggtaaagg tcgttttcta gaatgatcaa ggtctgcgct tcgcctgttt caatcgcctg
     8161 gaaagcctcc gccagcgtgc caccgcccat gagtccgagc ccaaagctgt tgctctcggg
     8221 aggtacgagg cagagcgcgc cgggtcggcc cgtccgacac aaagcccagg cgacgttggc
     8281 cgccgcctcg atgagagcgg cattcgcaca accgttgccc gcgacaatca gcggccgttc
     8341 agccgtaatg agcgcttccg caatctcacg cgccaggccg tcgatctctg gcgcaagctc
     8401 cggggccgct ggagctgcgg tgtcgatcat ccgggctacg gcgaagccga ggcgcgcgag
     8461 atcatcagga gctgctcgaa aacaacgcgt ggcaacgtcg tcgagccggc tcgcgacggg
     8521 tgtcgcaaga aaaataggac ttttctcgtt cccgccggct tcgcgaaccg cttggtcctg
     8581 ccagcgtgga atattgagcc gatctgcgat cgccagggat ttctgccgaa ccgcctggcg
     8641 caggctcagg gcaaggcggg gcgccgtatt tggtacgtcc tcacccagca ccagcaccgc
     8701 atctgcctct tcggcctcct tgagagaagc agcacgggcc ggaccatttc tcagaatcgc
     8761 cagcgtcaac atcagcaatt catgatcgct cttcgacaat ccgagataaa agcggtccgc
     8821 gccgaccagg gtacgcagcg caaagttggc ttcgagcgat gcacgcggcg agccgatgcc
     8881 aattgccttg ccggttccga tcatttccgc gacccgttgg agcgccgcct gcttggtcat
     8941 aggcgagaga gtaccgtctt tcctcaggag cggctcgcga acccgcagct cgctgtttgt
     9001 gaaaccgtac ccaaacctgc cgcgatcgca caagaaatag ccattaaccg cactattgta
     9061 gcggttcaga acgcgcttca attcccccgc gcgctcgttt accgtcgtgt tgcagccaag
     9121 gctgcaatgg atgcaaatgg acggcgctgc tcgcaaatcc catttgcggt tatagacccg
     9181 gctcaaggtt ttgtcgtcga ataccccggt cgggcagacc tcggccaggt tgccactgaa
     9241 ttcattctcg agaatgccat cctcgtagcg accgaaatag acattgcggt gaatgccaaa
     9301 aacgtcgaaa tcgcgccctc ccgcataatc ccggtaaaag cggacgcagc gatagcaggc
     9361 aatgcaccgg ttcatctcat gcgtcaggaa aggcccgaga tattggtttc gataggtgcg
     9421 cttcctgaac cgataacggc gatagacatg gccggtcatc agcgtcatgt cctggaggtg
     9481 acattcgccg ccttcctcgc aaactgggca gtcatgcggg tggttggtca tcagccactc
     9541 gatgatgctg gcgtggaacg ccttgacctc cggatcggcg atggagatcc gcaagccgtc
     9601 tgccgcagcc gtcatgcacg acatgacgat gcggccatgt tggtcgtcgg caccgtggaa
     9661 ttgctttacc gcacattggc ggcacgcgcc gactgatccc atcgctggat gccagcagaa
     9721 atacggaata tcgaaaccga gtgacaggca ggcatggaga aggttttgcc ctgtctccac
     9781 ctcataggac ttgccatcga tttcgattct gaccaaaggc tagctccacg gacagcgccg
     9841 ctcgtcgata tgacgctcga aatcggctcg gaaatacttc aacccgctct gaagcggctc
     9901 catcgcccca ggtgcaagcg cgcagaacgt cttgccgggg ccaaggaagc ccgtatgcat
     9961 cgcgagaata tcgagatcct gtgcttgtcc ctgccctgtt tcgatagccg cgagcatgcg
    10021 ctccacccac ggcagcccat cgcggcacgg cgtgcaccag ccacaggact cctgcgcgaa
    10081 gaaatgctcg agattgtgca gcatgccgac tggacaggtc cggtcgtcga gaatgatcat
    10141 ggtgccagtc cccaaccggc tgccgacttt ctggaccgcg ctgaaatcca tggggacgtc
    10201 gagatgttcc tcaagaagaa attcggtcga ggcgccgccc ggaaggagcg cgcgaaagct
    10261 gtagccttcg cgcatgccgc cagcgtgttc ttcaagaatc tctcgcgccg cggtccccat
    10321 cggcagctcc caaatcccgg ggcgtttcac acggccgctg acaccgtaga gcttcgtccc
    10381 cccatcttcg ctgcggctga gaccgcgaaa ccaggcagcg ccatgattga cgatgtgcgg
    10441 cacgttgcac aacgtctcga cattgttgac aatcgtgggc ttgccccaaa gtccgctcgc
    10501 ctgcggaaat ggcggctttg cgcgcggaat ggcgcggcgg ccttccaggc cattgagcag
    10561 cgccgtctcc tcgccgcaga tgtaccggcc tgcactggca tggagatgaa gctcaaggtc
    10621 gaagcccgag cccaaaatat ttgtgccgag atagcccttt tcagaagcct cggcgatcgc
    10681 gcgccggagg tgtcgggcgg acagcacata ctccgcacga aggaaaatat aggcgatacg
    10741 ggcctcgatg gcgaaagcgc tgaggatgac gctttcaata agctgatgcg gatccccttc
    10801 catcagcaac cggtccttga aggtgccggg ctccatctcg tcggcgttga cgacgagata
    10861 cttgggcgac ggtgcatcct ctcctcttgg tacgaagctc catttctggc ctgtcggaaa
    10921 tccggcgccg ccccggccgc ggagcttcga ttctgtcacc tcgtgcatga cctcgtcggg
    10981 catcatactg cgaagcgcct tgcgaaccgc cgaatatccg ccggcgcgct cgtagtcgcc
    11041 aatcgacaac ggttcttgtc cgggtcggat gtttcgggtc agcggccgtt ccacgtaaat
    11101 ccgtcccaag ttcagcgaaa tctcgcaagc acttcgtcaa gttgcccgat ctcgagatcc
    11161 ccgatgagat catcgtcgac catcatgacc ggggcatgat cgcaagcccc gaggcaaacc
    11221 gtcggcaaca gggtgaagcg accatctgcc gttgtttcac cggcttttat gtctaggcgg
    11281 ctttcaattc cgcgccgcaa cgcctcgcag cccatgatcc agcaggaaac actgtcgcaa
    11341 agcaggatga cgtgccgccc aacggggcgg cgaaaaataa gattgtagaa agtcgcaacg
    11401 gcgtcgagct cgtctgcaga catatcgagg aggcgcgcga catcccgcag gcattcatcc
    11461 gaaacccagc cgcgcgagcg ctgcacttca cgcaaggcct cgatcgaagc tccttgcttg
    11521 ttctcataac gtcgggcgat cgcttcgatc gcctcggttt cggcggtcgt cagcatcacg
    11581 agcgcctcat ctgtccacat ccgccatcac gaaatcgata ctcccgagaa tggcaatcag
    11641 atccgcgatc atggcctgac ggcacatcag cggaatcatc tgcagatgcg gaaatgatgg
    11701 cgtgcgtatg cgtgtgcggt aggacatcgt tccaccatcg ctgataagat aatagctgtt
    11761 cagccccttc gtcgcctcga cacaggcgct agcctcgccg ggtggaataa ccgggcccca
    11821 gctcacattg agaaagtggg tgatcagcgt ctcgatgtcg tgcatcgtgt gttctttgag
    11881 cggcggagtc gtcaacggat gcgccgcttt gtaggggccg gcgggcatat tctcgacgca
    11941 ttgacggata atgcggaggc tttgccgcat ctcttcgacg cgcacggccg tgcggtcgta
    12001 gcagtcacca tgaaggccgg tcggaatttc gaagtcgaag tgctcgtatc ccgaataggg
    12061 tcgtactttc cgtagatccc attcaagtcc acatgctctc aggcttgggc cggtggcgcc
    12121 ccactcgatc gcctcatcga ggctgtaggc gccgacaccc tgcgtacgca ttttgaaaat
    12181 gcggttcttc atgacgatct tgtcgtattc gtcgagccgg ggcggaagat aggctacgaa
    12241 ctcacgcacg agccggtccc atccttccgg cagatcctgg gccacaccgc cgatgcggaa
    12301 ccacgccggg tgcatgcgaa aaccacagat ggcttcgatg atctcgaaga cacgctcgcg
    12361 atccgtgaac atgtagaata ccggcgacag cgcgccgacg tcctgcgcca ttgtcccgta
    12421 gaacacgaga tggctcgcaa tccggaaaag ttcggccagc atgatccgga tcactttcac
    12481 ccggtctggc acctcgattc cggcgagctg ctcgacggcg agcacatagg gcagattgtt
    12541 catcaccccg ccgagataat cgacgcggtc cgtgtaaggg atataggtgt gccaggattg
    12601 gcgctcgccc attttttcgg cgccgcggtg gtgatagccg atgtccggca ccgcgtctat
    12661 gatgtcctcc ccatcgagct gaagcgccac gcggaaaacg ccatgcacgc tcggatgatt
    12721 cggtccgaga ttgaggaaca tgaagtcgct ggtttcgctg cgccgcttca tgccccaatc
    12781 ctcgggccga aaacgcagcg cgtcctgctc gctgtcctgt tttgattccg gcagctcaaa
    12841 aatgcccatt tccgtcgctc gggccgggtg atctttcctg agcgggtggc cctgccaggt
    12901 gggcggcatg agaattcgcc ggaggtgcgg atgcccctcg aagacaatgc cgaacatatc
    12961 ccagacttcg cattcgtacc agtcggcgga cctccacagg tccgtcaccg tcgggcacgt
    13021 cggcgtatcg cctgaaagcg ctaccttgat tcgaatgtca tcggccctgt cgtaagatag
    13081 gaggtgatag acaatggtga aatcgcccgt cggtccggga cgacgatgcg agcgatggcg
    13141 ctcatcgatc gcagtcaggt cgaaaagggt tcggtaaggc cgctcgattt tctccttcag
    13201 ataggccagg atttcatgcg ctcgttccgc cggcgtccag aaagtcggca ccgagtcgcg
    13261 cgtctcctgc ggccgtatgg cctcgacacc gaaacgtgcg gtcaaatcac ggaggacagc
    13321 agatgaatgc tggaggtcgg gatcgtcggc catcgcctca aacactatcc ggtgtacgca
    13381 gtacggtgac ttggctccgc tcggcccgct tgatgtcgcg catgctgggg cgtggcggcc
    13441 gctcgacacc ctgcggtccg acgacccagc tcaggggccg cctttccgtg ccgatctgtt
    13501 cctggagcag gacaagtccc tcgagcaggg cgtcaggacg cggcgggcag cccggcacgt
    13561 agacatcgac tggcaaaaag gtatcgaccc cttggacgac gctgtagatg tcgtacatgc
    13621 cgccggaatt cgcgcacgac cccatcgaaa tgacccagcg cggctccagc atctggtcgt
    13681 agagccgctt tatgaccggc gccatcttga tgaaaaccgt cccggaaatg acgatgagat
    13741 cggcctctcg tgggctggcg cgcagcacct ctgcgccgaa acgcgcgatg tcgtatttgc
    13801 tggtgaaggc cgtggccatt tccacgtagc agcacgaaag accgaaattg aagggccaga
    13861 tcgaattctt ctgtccccag gatacgatgt cctgaagccg ggtcaggata acgctgcgcc
    13921 tcacagcctc ttcgaacgag ctgctggtgc tcccgtttgg gcccgtagcc ggcggcctgc
    13981 tcagcgacca agtcatcgcc gcaattccct atcgagcggg atgagccgct cacgtcgggg
    14041 tccccaatcc agcgcgccgc atcgccataa atagacgagc gcagcgacaa gaaccccgat
    14101 gaagatcaac acttcgatat agccagccca gccgacgtct ctgaaggcga ttgcccaggt
    14161 gaatatgaag accgtttcga gatcgaaaat gacgaagagc atcgccacga gatagaattt
    14221 cgccggaaac cgcaaatgag cagaaccgac gctgacaatt ccggattcga atggctggac
    14281 ggtcgccttc gattcgtgcc gcgatccgag gagataagag ctgccgagca tcaccgcgat
    14341 cagcccgatg acggctgcgg aatacagcac cagcggccac agcggcatag gtatccccgt
    14401 caactgccga cccgctcctt ggccgacaac tcctgctccg aacaagcgtt cccgtccgca
    14461 ccctccttac ctggccttcc catgcatcac cgggagcgct gggggcgagc gccgcgatcg
    14521 cagattggtc tgctggcggc ggccgagtga atggagggtc agctcactag ccgagcgtct
    14581 gctttggtta acacggcccc atcacactgg tggccgtttc atccggagcg gtaccgaaat
    14641 tacttactct gacttgaatg gatggaaaaa tgccgcggca cgagaagcag tatcagcagt
    14701 atcagcaggc cgaccacgac catgacgagg aacaaatcgc gggtggcgac atcaatcgac
    14761 tggcgcagat aggtctccgc ggcggctccg atgtcggcgt gatggagcgc gccaatgacg
    14821 tcgttcacgt ttctgggcaa ctcatcggca actgaaaccg gcgcgttggc aaggcgaccg
    14881 gctacggtcg cattgaagac agcgccgaac agggctgcgc caagactctg gcccaaatag
    14941 cgcgagaaca tatttgcgcc ggtgacgacg ccgcgctggt tccagccgat caccgactga
    15001 acaccgacca atagcggggt agacaataag ccgaaacccg cgcctagcgt gatttggtct
    15061 atcaccaccg cccataccgg ctgggggctc ggcatgagca ggaacgcacc cgagccaagc
    15121 aatatcaaaa tcgatccgat aagggccgtg tcgcggaagc cgatacgcag gtacaggcga
    15181 ccggataatg ccgatgccgt gggccagccg accgacatgg tagcaagcac catgcccgcc
    15241 gcaatcggac caagaccttg caccgattgc aggaaggtcg tcaggtaggt cataggcgcc
    15301 atcatcacca atcccatgcc gaccacggca agattggatc cggccaggat acgtcgtcgc
    15361 caaagccagc caggcagcac gggctcggcg gcacgtcgct cagaccacac catcccggcc
    15421 agcagcacga cggcaattgc gaaaaccgca atgctctcgg gggaactcca cgcccaagcc
    15481 tgcccgccct ggagcaggcc gaagatcacc acgccaacgg atgtgagcac gagcaccgac
    15541 ccaagatagt cgatacgcgg atggcggcgc tcgaagtctt cgtgcaaaaa ggcgccgatc
    15601 agcgccagtg ccagtgcgcc gacgggcaaa ttaaccaaga atatccagcg ccatgaaacg
    15661 tattcgacga gtgcgccgcc cagaaccggc ccgaggaccg cggcaatgcc ccaaacgctc
    15721 gagagccatc cctggacggc ggcgcgttcg cgcaccgaat acaagtcacc tgccaatgtc
    15781 gacaccgtgg cgatgatcgc accagcgccc aagccctgaa tgccacgaaa cacgatcagt
    15841 gagaccatgt tccaggacag cgacgaggcg gcggagccgg ccaggaaaac caatgtaccg
    15901 atgatcagca cgggcttgcg gccgaacagg tccgacagct tgccgcaggt gggaatggtg
    15961 accgtctgcg cgagcagata gatggaaaac acccaactga acagccggaa gccaccgaga
    16021 tccgccacga tctgcggaac ggcggtggcc acgatcgtcg tatccatcgc agccagcatc
    16081 atcgtgcaca tcagcgccac gaggatcagg ccgcgcgggt tggtgtgatc acgttctcgc
    16141 actgtcatca tgaaagcgtc cggagcagtg gagggcgccc agggacgagg accaattcga
    16201 attggccctt agt
//
LOCUS       DASVDU010000050         7030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1283965, whole genome shotgun sequence.
ACCESSION   DASVDU010000050 DASVDU010000000
VERSION     DASVDU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283965"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..136
                     /locus_tag="VF226_03440"
     CDS             14..136
                     /locus_tag="VF226_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073070.1"
                     /translation="MARRWADPREIAYPILWLASDEASYITASAIMVDGGRFVL"
     gene            complement(144..809)
                     /locus_tag="VF226_03445"
     CDS             complement(144..809)
                     /locus_tag="VF226_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073071.1"
                     /translation="MKPLPRNRAADVAYDAIRQGVMSGRWPSGAHLREVELASELGVS
                     RTPVREALRRLASEGVLTFEPHIGATVPDWSAADLDEIFELRSRLESYAAELAASRAD
                     EATIAAMHEATRAMERAAFSGDVPDTVAVTANNDRFHQLIVNASGSKRLAAFIASVVE
                     LPLVVRTFARFDAHAMRRSIAHHDEITEAISHRDPIWAGAVMRAHIQAGRHVMLGTRA
                     AGE"
     gene            962..1909
                     /locus_tag="VF226_03450"
     CDS             962..1909
                     /locus_tag="VF226_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073072.1"
                     /translation="MSAFKWIFAAAAFAGSVLAAPLAAQAERITVTHWGVLMYGAPYA
                     VAIEKGFYKEAGVDVDGVLTSKGGGTTMRNVMAAEMPYGEVALAAVIAAINQGIELKI
                     VHTGVRTAGEILWATTPNSGISSVKDLAGKKVAFTSPKSVTDMLLTMVLDKYKIKAET
                     VAAGGIGSGLTMLDQGGIVAAPVMDPIWSKVQSKYKQVFAVADELPPMVQTVGVTTAE
                     FAKSNPDKIRALTAARRKGVEFIYSNPEEAAKIVAKHYEMDEALALQAIKNLSALKYW
                     SDGSFEYEGMDNMVKGLQIIGEVSGPVKWSEVVDESFVK"
     gene            1942..2748
                     /locus_tag="VF226_03455"
     CDS             1942..2748
                     /locus_tag="VF226_03455"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073073.1"
                     /translation="MATEIHAEMRGVTRIYPAADGKSGLHALGPVDLALRRGEFYSVI
                     GPSGCGKSTLLDVLAGLSRPTAGEVTFEGKPVEGRVPEGIGVVFQEDASFPWLNVEDN
                     IAFGLRRAGTDAKEVRRRLDHALALMGLTAFAKAYPAQLSGGMRQRVCIARTLVTQPR
                     LILLDEPFGALDAQTRLIMGDELLKLWRETGATVLLITHALDEAAMLSDRIGVMSARP
                     GKIIEEVATGWPSSRDSRVASDDHFGTITSRLWSHLREESLKAIGQGAKL"
     gene            2745..3494
                     /locus_tag="VF226_03460"
     CDS             2745..3494
                     /locus_tag="VF226_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073074.1"
                     /translation="MSRVGLLRFVIVAGFVLALEILCQTGVIRPTVMIPPSAMAVHLW
                     ALLVSGRATSDIVETLGNVFAAFALSVTVGFALGAVIHGRPRLRRAMDPFLATYYALP
                     FFAFYPLFIVLFGLGRPPIIAIGFLFGVVAMIIATLNGFDRLPRALIKTARVYRMNPI
                     STALRIKLPSAAPYLFTGVKLAIAYSFIGVIAGEFIMSSSGLGYSIAYAFNNFDNRTM
                     YALMLFVIVLVTIVNALFYVWEQKLLARRGR"
     gene            3494..4231
                     /locus_tag="VF226_03465"
     CDS             3494..4231
                     /locus_tag="VF226_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7073075.1"
                     /translation="MMRRAFDTALLVIGALLIWQALHLLAPTALTSPPGTAARAAELL
                     GTATFWPHVAETLSAFFYSLVLAFGGGLFIGLLLGLNRTGAKVADPILVSIYSLPKVT
                     LYPVILLIFGLGMPAKVAFGTIHGIIPIAIFAMNAVRNLAPVYLKTARVLHLSRAETL
                     RTVVIPAAIPEIVSGLRIGFSLTLLGVLIGEMFASQRGLGYMIMNAIGLHDVPTMMAI
                     ILMLALFAVAANAVLLWLDQRLHHRAA"
     gene            4252..5112
                     /locus_tag="VF226_03470"
     CDS             4252..5112
                     /locus_tag="VF226_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX7073076.1"
                     /translation="MSQSLVQRLKQPGLISAPGVFDMISAKIADGMGFDALYMTGFGT
                     VASYLGLPDAGLATYTDMVSRVRQIAGGTRTPLMADGDTGYGGLLNVDFTVRGYEAAG
                     AAAIQIEDQEYPKKCGHTPGRRVIPIEDAARKIKVAAEARSSKDFLIIARTDARTTLG
                     LDEALRRGEAFAKAGADILFIESPESMEEMRRIGETFDIPLVANMVEGGRTPIMSRAE
                     LEECGFKLAIFPASGFLAMGAALQSVYKEIREKGSSKDWNGALYPFGDFTKLMGFERV
                     WEFERRNAEI"
     gene            complement(5149..5934)
                     /locus_tag="VF226_03475"
     CDS             complement(5149..5934)
                     /locus_tag="VF226_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073077.1"
                     /translation="MLTLENVEVVYDQVFLAIKGVSLEVAEGGAVALLGSNGAGKSTT
                     LKAISGLLAPERGKVTRGEIRFRGQDLLGLSAPERVRLGIAHVLEGRHVFGHLTPLEN
                     LIAATSMHRSRAKINELTERVFEWFPRLKQRSNAQAGYLSGGEQQMLAIGRALMTEPK
                     LLMLDEPSLGLAPFLVDEIFDIISRIVRETGMSILLVEQNATAALAIVDHAYLIDNGR
                     VVMSGASEDLRQNPDVQEFYLGGTGRVDYRNVKHYRRRKRWLA"
     gene            complement(5954..>7030)
                     /locus_tag="VF226_03480"
     CDS             complement(5954..>7030)
                     /locus_tag="VF226_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073078.1"
                     /translation="TLINERGGINGNRFEAMVVDTGNEPQRGIEAYERFKAEGAVLVD
                     FLSTPVSRAVVPRALKDGINVLTMFHGRSDAADGETFPTIFPAGALYWSQAAVILKYI
                     SDQEGGNLKGKKIAFVGIDSPFGKEPVPVFEAVRDKEGFELQTFFYPSPGNEQSSTWT
                     AVRRYRPDWVVIWGAGGGQTVSLRDAIRNGIKLDHLLSVIWLSETDTQIVGNEAAKGV
                     LRFEGVAYGRDNPVVKAILDEVYAKGKGAGDEKNVGSSYYNIGVASMAIVVEGVRNAL
                     EKQGEPLTAEKLKKGLEEIKNFTAEGLLPPLTITAQDHQGGGYGRISQWDGSKWVPKT
                     DWIAAYQDVVMDLVKKTAAEFKKQ"
BASE COUNT         1239 a         2179 c         2248 g         1364 t
ORIGIN      
        1 ggccggcggc tgtctggctc ggcggtgggc ggacccgcgc gagatcgcct atccgatcct
       61 gtggctcgcc tcggacgagg catcttacat tacagcttcg gcgatcatgg tcgacggcgg
      121 ccggttcgtg ctctgaagtc ccttcactct cccgccgcgc gagtcccgag catcacgtgc
      181 cgtcccgctt ggatgtgcgc gcgcatcacc gcgccggccc agattggatc gcggtgactg
      241 attgcttcgg tgatctcgtc gtggtgggcg atcgagcggc gcatcgcgtg ggcatcgaag
      301 cgcgcgaacg tgcgcacgac gagcggcagc tcgacgacag aggcgatgaa tgccgcgagc
      361 cgtttgctgc cggatgcgtt gacgatcagt tggtggaagc gatcgttatt cgcggtgacg
      421 gcgaccgtgt cgggcacatc gcctgagaac gcagcgcgct ccatggcgcg cgtcgcctcg
      481 tgcatggccg ctatagttgc ctcgtcggcg cgggaagcgg caagctccgc agcgtagctt
      541 tcaagccggc tcctgagctc gaatatttcg tcgagatcgg ccgcgctcca gtccggcacg
      601 gtagcgccga tatgaggctc gaacgtcagc acgccctcgc tggccaggcg tcgaagcgcc
      661 tcgcgcacgg gagtccggct cacccccagc tccgatgcaa gctcgacctc ccgaagatgt
      721 gcgcccgacg gccaacgccc cgacatgacg ccttggcgta tggcatcata ggcgacatcg
      781 gcggcacgat tgcggggcag gggcttcacg cgcatttcca ctggacaggg tgtctcattg
      841 tatacaatct agccgagggg gcgcataggg gccaagaaag aaactatcgg cgccggatgc
      901 gcacagaagc ccgacagccc ctcgcgccga gtcagtcgtc gtccatggag gaaatcagct
      961 catgtcagct ttcaaatgga tttttgcggc tgccgcgttt gccggatcgg tgctggccgc
     1021 accccttgct gcacaggcgg agcgcatcac cgtcacgcac tggggtgtcc tgatgtacgg
     1081 cgcgccttat gcggtggcca tcgagaaggg cttctacaag gaagccggtg tggacgtcga
     1141 tggcgtgctg acttcgaagg ggggcggcac gacgatgcgc aacgtcatgg cggccgaaat
     1201 gccttatggg gaagtcgcgc tggcagcggt cattgcggcc atcaatcagg gcatcgagct
     1261 caagattgtg cacacgggcg tgcgcacggc gggtgaaatc ctctgggcga cgacgccgaa
     1321 ctccgggatc agctccgtca aggacctagc cgggaagaag gtggccttca cttcgccgaa
     1381 gtcagtcacc gacatgctgc tcaccatggt gctcgacaag tacaagatca aagccgagac
     1441 cgttgcggcc ggcgggatcg ggtcgggcct gaccatgctc gaccagggcg gcatcgtcgc
     1501 cgcgccggtg atggatccga tctggtccaa ggtgcagagc aagtacaagc aggtctttgc
     1561 ggtggccgat gagctgccgc cgatggtgca gacggtcggc gtgacgaccg ccgagttcgc
     1621 caagagcaac ccggacaaga tccgtgcact caccgcggcc cgtagaaagg gagtcgagtt
     1681 catctactcg aatccggagg aagcggccaa gatcgtcgcg aagcattatg agatggacga
     1741 ggcccttgcc ctgcaggcca taaagaacct gagcgcgctc aagtactgga gcgacggcag
     1801 cttcgagtat gagggcatgg acaacatggt gaaagggctc cagatcatcg gcgaggtcag
     1861 cggcccggtg aagtggagcg aggtggtcga cgagagcttc gtgaagtgac atcgacgcaa
     1921 gtcgcaggcc ggagccgggc catggcaacc gagatccatg ccgagatgcg cggtgtgacg
     1981 cgcatctacc cggcagcgga tggaaaatcg gggctccatg cgctcggtcc ggtcgacctg
     2041 gcgctcaggc ggggggagtt ctattcggtc attggtccct cgggctgcgg aaaatccacc
     2101 ctgctcgacg tcctggcggg cctgtctcgc cccacggctg gcgaggttac gttcgagggg
     2161 aagccggtgg agggccgcgt gccggaaggg atcggcgtcg tcttccagga ggatgcctcg
     2221 ttcccgtggc tgaacgtgga ggacaacatc gccttcggcc tgcggcgggc agggacggat
     2281 gccaaggaag tccgccgccg gctcgaccat gcactggccc tcatgggcct gacggcattc
     2341 gcgaaggcct atcctgcgca gctttcgggc ggcatgcggc agcgagtctg catcgcgcgc
     2401 acgctggtca cacagccgcg tctcattctg ctggatgagc ccttcggcgc gctcgatgcg
     2461 cagacgcggc tcatcatggg cgacgagctg ttgaagctgt ggcgcgagac cggggcaacc
     2521 gtgttgctca tcacccacgc cctggacgag gccgcgatgt tgtcggaccg catcggtgtc
     2581 atgtccgcgc ggcctggcaa aatcatcgaa gaggtggcga cgggctggcc gtccagccgc
     2641 gacagccgtg tggcgtcgga cgatcacttt ggcacgatta ccagccggct ctggtcgcac
     2701 ctgcgcgagg aatccctgaa ggcgatcggg cagggggcga agctatgagc cgggtcgggc
     2761 tgctgcggtt cgtgatcgtt gccgggttcg tcctagcgct cgagatcctg tgccagacag
     2821 gtgtaatccg cccgaccgtg atgattccgc catccgccat ggcagtgcac ctgtgggcgc
     2881 tgctggtttc cggtcgtgcc acgtccgata tcgtcgagac gctcggaaac gtattcgccg
     2941 ctttcgcact gtccgtaaca gtcggatttg cattgggcgc ggtcattcat gggcggccac
     3001 ggctgaggcg cgcgatggac ccgttcctcg caacctatta cgcgctgccg ttcttcgcct
     3061 tctatccgct tttcattgtc ctgttcggtt tggggcggcc gccgatcatt gccatcggct
     3121 ttctgttcgg cgtcgtcgcc atgatcatcg cgacgctcaa cggattcgat cgtttaccgc
     3181 gggcgctgat caaaacggcg cgtgtctacc ggatgaatcc gatctcgaca gccctcagga
     3241 tcaagctgcc gagtgcggcg ccatatctct tcacaggcgt gaaacttgcc atcgcctact
     3301 cgttcatcgg cgtgatcgcg ggcgagttca tcatgtcctc ctcagggctc ggctattcca
     3361 tcgcctatgc cttcaacaac ttcgataacc ggacgatgta cgcgctgatg ctgttcgtga
     3421 tcgtgctcgt cacgatcgtc aacgcgctgt tttacgtctg ggagcagaag ctgctcgcgc
     3481 ggcggggacg gtgatgatgc ggcgcgcctt cgatacggca ctgctcgtta tcggagccct
     3541 cctgatctgg caggcgctgc atctgctggc ccccacggca ctcacttcgc caccaggtac
     3601 ggcggcgcgg gcagccgagc tgctcggcac cgcgacattc tggccgcacg ttgcggagac
     3661 gctgtcggca tttttctatt cgctggtgct ggcgttcggt ggagggcttt tcatcgggct
     3721 tttgctcggc ctcaaccgca cgggcgcgaa ggtggccgat cccattctcg tttccatcta
     3781 ttcgctgccg aaggtcacgc tttatccggt cattcttctg attttcggac tcggcatgcc
     3841 ggcaaaagtc gcgttcggga caatccacgg catcattcca atcgccatct ttgccatgaa
     3901 cgccgtgcgc aacctggcgc cggtatatct gaagacggcg cgcgtcttgc atctgagccg
     3961 ggctgagacg ctgcgtacgg tcgtcattcc cgccgccatt ccggagatcg tttccggact
     4021 caggatcggg ttttcactga cgctcctcgg cgtgctgatc ggcgagatgt tcgcctcgca
     4081 gcgtggcctc ggctacatga tcatgaatgc catcgggctg catgacgtcc cgaccatgat
     4141 ggccatcatt ctcatgctcg ctctctttgc ggtcgctgca aacgcggtcc tgctctggct
     4201 cgatcagcgt ctgcatcacc gggccgcttg aacactggga agaacaaatc catgagccaa
     4261 tctctcgtcc agcgcctgaa acaacccggt ctcatctctg cgcccggcgt cttcgatatg
     4321 atctcggcga agatcgccga cggcatgggc ttcgacgcgc tttatatgac cggcttcggc
     4381 acggtggcct cctatctcgg attgccggat gcggggcttg cgacctatac ggacatggtc
     4441 tcacgcgtcc ggcagatcgc gggcggcacg agaacaccgc tgatggccga cggcgatacg
     4501 ggttacggcg ggctgctcaa cgtcgacttt accgtgcgcg gctacgaggc ggctggtgct
     4561 gcggctatcc agatcgaaga ccaggaatac ccgaaaaagt gcggccacac gccgggacgg
     4621 cgcgtcatcc caatcgagga tgcggcgcgc aagatcaagg ttgccgccga ggcgcgctcg
     4681 tcgaaggact ttctgatcat cgcccgtacg gacgcacgca ccacgctggg cctcgacgag
     4741 gcattgcggc gcggcgaagc cttcgccaag gcaggcgcgg acatcctgtt catcgagtcg
     4801 cccgaaagca tggaggagat gcgccgcatc ggagagacgt tcgacatccc gctcgtcgcc
     4861 aacatggtcg aaggcgggcg cacccccatc atgagccgtg ccgagctcga ggaatgcggc
     4921 ttcaagctgg ccattttccc agcttccggc ttccttgcga tgggagccgc attgcaatcc
     4981 gtctacaagg aaatccgcga gaaaggctcc agcaaggatt ggaatggcgc gctttacccg
     5041 ttcggtgatt ttaccaagct catggggttc gagcgcgtat gggagttcga gcggaggaac
     5101 gcggagattt gacgtcctcg ggttccgaca cgctgaggcc ggctgcgctt acgccagcca
     5161 ccgcttgcgg cggcggtagt gcttgacgtt gcggtaatcg acccgtccgg tcccaccgag
     5221 gtagaattcc tgcacgtcgg ggttctgccg caagtcttca gacgcaccac tcataacgac
     5281 gcggccgttg tcgatcaggt aggcgtggtc gacgatggcc agcgcggcgg tggcgttctg
     5341 ctccacgagc aggatgctca tgcccgtctc gcgcacgatc cgggagatga tgtcgaagat
     5401 ttcgtcgacg agaaacggtg ccaaaccgag gctcggctcg tccagcatca agagcttcgg
     5461 ctccgtcatc agagcacggc cgatggccag catttgctgc tcgccgccgg aaagatagcc
     5521 ggcctgagca ttggagcgct gcttcaggcg cggaaaccac tcgaaaacgc gctcggtcaa
     5581 ttcgttgatc ttggcccggc tgcggtgcat cgacgtcgct gcgatcagat tctccagcgg
     5641 cgtgaggtgc ccgaagacgt gacggccttc gagcacgtga gcaatcccga gccggacccg
     5701 ctcaggcgcc gaaagcccca gcaggtcctg gccgcggaaa cggatctcgc cacgggtcac
     5761 ctttccgcgc tccggcgcca gcaggccgga aatggccttg agcgtcgtgc tcttgccggc
     5821 gccgttcgag ccgagcagcg cgaccgcgcc tccctcggcg acctcgagcg agacgccctt
     5881 gatcgcgagg aagacctgat cgtacacgac ctcgacgttt tcgagtgtca gcatcgctgg
     5941 atcgccgaga ggcctactgc tttttgaact cagcagcggt tttcttgacg aggtccatca
     6001 cgacgtcctg gtaagcggca atccagtcgg ttttcggcac ccacttgctg ccgtcccact
     6061 gggagatgcg gccgtagcca ccgccctggt ggtcctgagc cgtgatggtg agcggcggga
     6121 gcagaccttc ggcagtgaag ttcttgatct cttccaagcc cttcttcagc ttttctgcgg
     6181 tcagcggctc accttgcttt tcgagcgcgt tccgaacgcc ctcgacgacg atggccatgc
     6241 tggcgacgcc gatgttgtaa tagctgctgc cgacgttttt ctcgtcaccc gctccttttc
     6301 ctttggcgta aacctcgtcg agtatggcct tgaccaccgg attatcgcgc ccgtaggcca
     6361 cgccctcgaa gcgcaacacg cccttggctg cttcgttgcc gacgatctgg gtatcggttt
     6421 cagacagcca gataacggac agcagatgat cgagcttgat cccgttccgg atggcatcgc
     6481 gcagagacac ggtctggcca ccgcccgcgc cccagatcac gacccaatcg ggacgatagc
     6541 ggcgaacggc ggtccaggtg gaggattgct cattacccgg agatgggtag aaaaacgtct
     6601 gcagctcgaa gccttccttg tcgcgcacag cctcgaagac cggaacgggc tccttaccga
     6661 acggcgagtc gatgccgacg aaagcgatct tcttgccctt caggtttccg ccttcctgat
     6721 ccgagatgta cttgaggatc actgcggcct gcgaccagta gagagcgccg gcggggaaaa
     6781 tcgtcgggaa tgtctcgccg tccgcggcgt ccgagcggcc gtgaaacatg gtgagcacgt
     6841 tgatgccgtc cttcagtgca cggggcacaa cggcgcgaga gacgggcgtc gaaagaaaat
     6901 cgaccagaac agcgccttcc gccttgaagc gctcgtaagc ctcgatgccc cgttgcggct
     6961 cgttgccggt gtcaaccacc atcgcttcga agcggttgcc attgatcccg ccgcgctcgt
     7021 tgatgagggt
//
LOCUS       DASVDU010000051        26098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_511462, whole genome shotgun sequence.
ACCESSION   DASVDU010000051 DASVDU010000000
VERSION     DASVDU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26098
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511462"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..920
                     /locus_tag="VF226_03485"
     CDS             264..920
                     /locus_tag="VF226_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073079.1"
                     /translation="MPLLGKGMLITSMDVDPAEEADFNLWYDREHLAERVAIEGFLEA
                     RRWIADEAPTKYFCTYSTDTFEALSSPAYQKALANQTDWSKHHISRFRNPGRAVGRIT
                     VSRGQGRGGVLGVVRLRPGADADKAALRERLRELLDPGMLPGIISMHLVESDPTLSQN
                     LTDPNAPNPGAADWYVLIDGSDLAAVRALMNDRFSQPGAEIVSKATYRLLWDLAKSDL
                     "
     gene            969..1955
                     /locus_tag="VF226_03490"
     CDS             969..1955
                     /locus_tag="VF226_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073080.1"
                     /translation="MNRTVKSLLVAGSVAATLVASTAAFAERWNMPVRSNERNYFTRN
                     IMQFADEVKKATDGKLEIVVHPEDSLIKQPEVKRAVQTGQVQIGELLMSMHSNENAIF
                     GVDSVPFMAKNFEEGRKLLGITRPYLENLLKQQNMRLLFVVPWPANSFYVDKEVNSTK
                     DFQGLKFRAFNPVTGRLAELMGAIPVTVQQSEVSQAFSTGVIQAMITSPATGVDTQAW
                     DFVKYFYEVRAMSPWNIVVVNERMFRRLDEKTQKAVLDASKAAEERGWELAASETGKL
                     IDTLKEHGMEVRQPSPELMAELSKIGDTLIDEWIKSAGADGEKIMNEFRGEN"
     gene            1968..2483
                     /locus_tag="VF226_03495"
     CDS             1968..2483
                     /locus_tag="VF226_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073081.1"
                     /translation="MLTALNRIYAAGAVLAALCMVAIALLTLAQIMGRLFGVLIVDAG
                     ELAGFAMAGSIFFALAHTLRTGGHIRVNLLLARMRPSIRRIFEIWSLGLATTISGMFA
                     VFSVRMVIESYEFHDVSTGMIPVPLWIPQLSMAAGAVLFVVALLHELILTIRGQEAGY
                     VAAENTEPYTE"
     gene            2491..3795
                     /locus_tag="VF226_03500"
     CDS             2491..3795
                     /locus_tag="VF226_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7073082.1"
                     /translation="MDAIFYSPILIVALLGLLASGVWIAITLAGVAMIAMLLVEAPID
                     LVMPSSIWSAVSSWSLTALPLFIWMGEILFRTRLADDLFHGLAPWVNRLPGRLLHVNV
                     IGCGIFAAVSGSSAATAATVGQMSLPELKRRGYDEKLSIGSLASSGTLGLLIPPSIIL
                     IVYGVSANVSIARLFLAGVVPGLMVVALFMAYIVIWALLNREKMPPAEAGLSFWQMVS
                     RLRLLLPTVLLIIAVIGSIYTGVATATEAATLGVVGSLLIALLSGSLNWQTFRMSLYG
                     ATRTSCMIGFIIATAAFLSVAMGYAGIPRALASWIGTFNLSAYQLIFALAILYIILGC
                     FLDGISMVVLTTAVIMPMVQAAHIDLLWFGIFIVLVVEIAQLTPPVGFNLFVVQGLTG
                     HDILYVARSVLPFFLLLCATVAILTVFPEIATWLPRTMFSAR"
     gene            3835..5229
                     /locus_tag="VF226_03505"
     CDS             3835..5229
                     /locus_tag="VF226_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7073083.1"
                     /translation="MAQSDPTQLTASQLAADISARRLSPVDVVDAYLERIAAKDSKLH
                     SYVQVYAEEARLAAEAADRAIRAGHAVGPLHGVPIALKDLVEIEGKVTTGGCAVWRNR
                     VSTYTATLAKRLMAQGMIVLGKTHTVEFAMGGWGTNTHLGTPWNPWDLERLRTPGGSS
                     AGSGVSVAAGLAPWAVGTDTGGSVRLPASWCGITGLKTTIGRVSTYGVLPLSPTLDTP
                     GPMTRTVEDAALLYTVMQGEDPMDVRTRGVPYTDPMPTLKRGVRGLRLARMPQSERQF
                     ASDEVLAAYDRSLDELARLGAEIVDVTLPFALADVAALNLRIMAAESYAILHELIDDE
                     STQLDPDVRPRIAAGRSVTAKEYLEALQAKEDRKRQFLAALDGIDALLTPTTMTTAIP
                     VDEVDQKTAPAHFTRFGNFLELCGVALPNGFDSKGLPTSLQIACRPYDEATALRIGWA
                     YQNATEWHLRRPSV"
     gene            5393..6241
                     /locus_tag="VF226_03510"
     CDS             5393..6241
                     /locus_tag="VF226_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073084.1"
                     /translation="MSTRDQFVGLGERLYRVERPWGKLPSHLSYAGITDVTVLPSGRI
                     AVLLRSDPAVLVFDPDGTLADQWSLPDVVAGHYIRAMPSGRVLLADFDGHRIFVLRGD
                     DGRCEKILGARDRPRFGQPFNHPTDAVEAPDGELYVSDGYGNSCVHRFSADGTWIATW
                     GKPGSGKLEFSTPHAVVFDRAGRLLVGDRENNRVQILSRDGQWQGEISGLFKPMDVEP
                     TPDGGVLVTDQTPRLSYFSAEGVLIGRCRTFGTVGHGIALAPDGSIFVAEMGPDMLTR
                     FAPVTV"
     gene            complement(6321..6641)
                     /locus_tag="VF226_03515"
     CDS             complement(6321..6641)
                     /locus_tag="VF226_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073085.1"
                     /translation="MRTPALAALSLAIPLLAAAGGTPAVAGGWWGCDRPAAYYSAPVR
                     PYRYSYYAPSYYVPARPYYWGYGPRIVGYGPGPYYRYRRPLGFYSAPYAYAYGPSVSI
                     AVGF"
     gene            6876..7703
                     /locus_tag="VF226_03520"
     CDS             6876..7703
                     /locus_tag="VF226_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7073086.1"
                     /translation="MTFDLSSILWLLLVFAVLQPLLVSRWLTMRRAQAIRAMEKARGT
                     RVITMIHRQERRSLLGLSVSRHIDMEDAQTIIAAIKETPPDVPIDLVIHTPGGLVLAA
                     MQVARAIEAHPAKVTVFVPVYAMSGGTLIALAADEIVLGEFSVLGPIDPQIAGLPAAS
                     IVKARDSKPPESSFDLTLVLADVAEKALAQVKRGAVELLTPRLDQASAEALADKLAGG
                     HWTHDYALTATEAQDLGLPVKVGMPKTVLELMKLYPQPIMPSSVEFLPIDVPQKRRI"
     gene            complement(7713..8429)
                     /locus_tag="VF226_03525"
     CDS             complement(7713..8429)
                     /locus_tag="VF226_03525"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX7073087.1"
                     /translation="MHIHRRFLTTAAAAATLLCAVGANAATDPKGVWIDHTGRGAVEI
                     TDCNGALCGRVVWLKDTENAEACGIQILGDVKPVGAGVWDKGWIYDPEQDARFSVELK
                     PIGEEKLQVTGYLGTKLLSETMTWTRAPADLQRCAGDIGTTAALSPNEDAGTPAAAGT
                     AISPSNGTAASPEGNKSKVESKTGAETKSAAADGKTVPRSSTASKTEPKSDQPARSAS
                     KPQECAIRTPWVSFSFPCPD"
     gene            8818..10329
                     /locus_tag="VF226_03530"
     CDS             8818..10329
                     /locus_tag="VF226_03530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7073088.1"
                     /translation="MPTKTFAELGLSQKVLAAVEAAGYTSPTPIQAEAIPVALTGQDV
                     LGIAQTGTGKTASFVLPMITRLESGRARARMPRSLILAPTRELAAQVAQSFEKYGINQ
                     KLTVALLIGGVSMDDQTRKLDRGVDVLIATPGRLLDHFQRGRVMLMGVEILVIDEADR
                     MLDMGFIPDIEKICKLLPPKRQTLFFSATMPPEIQRLVDHFLTNPARIEVAPPATTVK
                     AITQRFVFCRDSEDWAKREQLRQLIRSQDVRNAIIFCNRKRDVAILNKSLLKHGFNAG
                     ALHGDMDQKSRMETLDAFRSGEITILAASDVAARGLDIPEVSHVFNFDVPWAADDYVH
                     RIGRTGRAGREGYAATLVTREDLKLVKQIEQVTGEQPTWIGEPPTEEDYAEAARKRRG
                     RRNGKSDARRANGRAEPKPKRERQRREKSAPAQPAAEAQPPQKEAAEPLETAPRREKP
                     RTKPRAVETPQPAPEHRTRQPRERRPESANVGFADKIPAFMRKPVRVAKAANE"
     gene            complement(10354..10749)
                     /locus_tag="VF226_03535"
     CDS             complement(10354..10749)
                     /locus_tag="VF226_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX7073089.1"
                     /translation="MGSSAEFLELLRDQLSGLGQIQVRRMFGGAGLYSDGVMFALVSD
                     DTLYFKTDEANGGCFDAESLPPFSYGTKTGRTTISSYRRAPDRLLDDPDELVAWARGA
                     LAAARRAQRAKPKRMGTSAGSSARIARMK"
     gene            complement(10764..11351)
                     /locus_tag="VF226_03540"
     CDS             complement(10764..11351)
                     /locus_tag="VF226_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX7073090.1"
                     /translation="MTDTARDTEASAGGEEAWVEEVLKFWFEELPHEDWFRKSDATDE
                     LIRSRFMNVHERVAALPADAAWTSPRRALATIIVLDQFPRNMFRGTPRAFATDGKAVE
                     VARGAVERGFDKAFDKDGRAFMYLPFEHSEDLADQERAVELFTPLGDPEYTRYAIAHR
                     DIIARFGRFPHRNAVLGRQSTPEEVEFLKQPGSSF"
     gene            complement(11412..11798)
                     /gene="sufA"
                     /locus_tag="VF226_03545"
     CDS             complement(11412..11798)
                     /gene="sufA"
                     /locus_tag="VF226_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HEX7073091.1"
                     /translation="MATARPRPKVMTLTDAAAERVRQIMASRPDVVGLRVGVKNGGCA
                     GMEYTMSWATEQDKLDEVVEDKGVRILIDPKAILFLLGTEMDYKVDKLSSQFVFNNPN
                     QSSACGCGVSVTITPASKESLDTVRG"
     gene            complement(11889..12344)
                     /locus_tag="VF226_03550"
     CDS             complement(11889..12344)
                     /locus_tag="VF226_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEX7073092.1"
                     /translation="MDEKHRERDVLEFISESPSERAEPDTERRSAPSVAAGGTPVEGS
                     ALSSEELEQLTADIIGALKTVYDPEIPADIYELGLIYKIDVDDNRNVSIEMTLTAPGC
                     PVAGEMPTWVENAVCSVPGVSGCTVTLTFDPPWDMSRMSDEARLAINMF"
     gene            complement(12367..13617)
                     /locus_tag="VF226_03555"
     CDS             complement(12367..13617)
                     /locus_tag="VF226_03555"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX7073093.1"
                     /translation="MTQTAATATGRRFDVERYRADFPILFREVYGKPLVYLDSAASAQ
                     KPLSVIEAMDHTLRFEYANVHRGIHLLSNTATQKYEDARETVRRFLNAPSAEEIVFTK
                     NATESINLVAYSFGLANIQEGDEIVLSIMEHHSNIVPWNYLRERKGAVLKWVPISDRG
                     EFLLDEFERLLSPRTKIVAVTHVSNVLGTVVPVNEVCRIARERGIPVLIDGSQGAVHL
                     PVDVQDIGCDFYAFTGHKVYGPSGIGVLWGRKALLEALPPFLGGGSMIESVTVDGITY
                     GQLPNKFEAGTPPIVEAIGLGAALNYMMQIGRDEIAAYESELVKYAHERLGDYNWLKI
                     FGTAPDKGAIVSFSVEGLHPHDVSTIVDRSGIAIRAGHHCAQPLMDRFGVPALCRASF
                     GLYNTKADVDALAAALVRAHEFFA"
     gene            complement(13632..14966)
                     /gene="sufD"
                     /locus_tag="VF226_03560"
     CDS             complement(13632..14966)
                     /gene="sufD"
                     /locus_tag="VF226_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX7073094.1"
                     /translation="MNVGLTKTKAEQAFTETFDAVVARLPGSGAVRELRRQAIGTFAA
                     LGLPHRRIEEWKYTDLRSFLKEAFAPAAGRPAPLSEADINAALGPLAEIEAYRILVVD
                     GAFDAGLSAKQLPDGLTVKPLSEALAESPEKAENLVRMKRDTDAIEALNSAFATDGAV
                     VRVADNTVLDKPLLLVFARAGKDRKSVTTRNVVDIGKGAKVTLIEAFVPLNGAADEDQ
                     HNAATEIAVGDGAAVAHIKAIIENGKAVHLGSATAEIGAEASYRAFQLTAGPGLARNQ
                     IFVTFNGEGSKLDCSGAFLAANNEHIDTTLVVDHAVPHCESRELYKGVLDGRGRGIFQ
                     GKVIVQPGAQKTDGKQMAQALMLSPDAEFDSKPELEIYADDVICGHGSTSAQIDPDHI
                     FYCRSRGIPEPEARALLIEAFVGEAMDKVEDEAVRDALMETARRWLNQRTAS"
     gene            complement(14988..15740)
                     /gene="sufC"
                     /locus_tag="VF226_03565"
     CDS             complement(14988..15740)
                     /gene="sufC"
                     /locus_tag="VF226_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX7073095.1"
                     /translation="MLEIRNLHVKIADEDTEILKGVDLVVPKGEVHAIMGPNGSGKST
                     LAYVLAGRDDYEVTEGDILWNGESLLEMEPDERAAKGVFLAFQYPMEIPGVAGLTFLR
                     TALNALRKKRDQAELTTPEFMKLVRDRAAKLNIDVEMLKRPVNVGFSGGEKKRSEILQ
                     MALLEPTMCVLDETDSGLDIDALRIVADGVNALRSPDRAMLVITHYQRLLNYIVPDKV
                     HVLAHGRIQRTGDKELAVELERSGYAEFTKAA"
     gene            complement(15799..16074)
                     /locus_tag="VF226_03570"
     CDS             complement(15799..16074)
                     /locus_tag="VF226_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073096.1"
                     /translation="MSSDVAQLEQAWLQAETAADALKQEALKAHAELARMQQALGGDG
                     NDVSVLVASIEQLKARQEEAERTASAAFDRYWMAQQGNGKDTGSAYA"
     gene            complement(16208..17680)
                     /gene="sufB"
                     /locus_tag="VF226_03575"
     CDS             complement(16208..17680)
                     /gene="sufB"
                     /locus_tag="VF226_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018483742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7073097.1"
                     /translation="MMAVQETIEQVRKIDVDQYKYGFETKIEMVMAPKGLNEDIIRFI
                     SGKKGEPEWMLEWRLEAYRRWRTMTEPTWAKVRYPKIDFEDIHYYAAPKSMEGPKSLD
                     EVDPELLRTYEKLGIPLKEQEVLAGVQGAPKVAVDAVFDSVSVVTTFKEQLAKAGVIF
                     CPISEAIREYPELVKKYLGSVVPQSDNFYAALNSAVFSDGSFVYVPEGVRCPMELSTY
                     FRINAQETGQFERTLIIADKGAYVSYLEGCTAPTRDENQLHAAVVELIALDDAEIKYS
                     TVQNWYPGDKDGKGGVYNFVTKRGDCRGRNAKISWTQVETGSAITWKYPSCILRGDNS
                     RGEFYSIAISNGYQQVDSGTKMIHLGKNTSSRIISKGIAAGYSQNTYRGLVSAHRKAT
                     NARNFTNCDSLLIGDKCGAHTVPYIEAKNSTAHFEHEATTSKISDNQLFYCMQRGLSE
                     EEAVALIVNGFVRDVIQQLPMEFMAETQKLIGISLEGSVG"
     gene            complement(17718..18851)
                     /locus_tag="VF226_03580"
     CDS             complement(17718..18851)
                     /locus_tag="VF226_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX7073098.1"
                     /translation="MTFARTYLDHNATAPLRPEARAAMLDALDTLGNPSSVHAEGRRA
                     RAIVEEARERVAALVGVTPAQVVFTSGATEANCAVLRAGWESILVAGIEHDSVLAPAR
                     ASGAAIVEVPVDANGRTELPTISSALDRSGSGKSVLALQLANNETGVIQPVAEAAAVA
                     HEHGVCVHSDAVQAAGRMELDFETLGVDTLSLSAHKLGGPKGVGALVLREGFDIAPFV
                     VGGGQERRRRAGTENVPGIAGFGAAASAAKRDLADMGRIGALRDRLESGALAMAPDAL
                     AIGSGAERLPNTTCLALPGLSAETLVIRFDLAGVAISAGAACSSGKVGVSPVLTAMGL
                     PEDVARSAIRVSLGWTTTEADVDEFLAAWGSIVGHGGRERAVA"
     gene            complement(18848..19276)
                     /locus_tag="VF226_03585"
     CDS             complement(18848..19276)
                     /locus_tag="VF226_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7073099.1"
                     /translation="MAMADLASHEGEGAVPLSAIAERQHLSIAYLEQIFLKLRRAGLV
                     ESARGRAGGYRLGRLASEITVAEIMHAVEEETRMTRCMGDDDVGCIGEEKCIAHNLWH
                     ALGDHIRVFLSNVSLREVLDGVPPEKRAPLPASKMDAAAQ"
     gene            19612..20286
                     /locus_tag="VF226_03590"
     CDS             19612..20286
                     /locus_tag="VF226_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7073100.1"
                     /translation="MPELIINGPAGRLEARYHHEPARDSPIALILHPHPQFGGTMNNQ
                     VVYHLYYTFAERGFSVLRFNFRGVGRSQGFFDNGPGELADAASALDWLQMAKPEAKSC
                     WIAGVSFGTWIAMQLLMRRPEIDAFICVAPPANLYDFSFLAPCPASGLLVNGERDRVV
                     PSAAVAELAAKTKTQRGIKLEHEIIPGANHFFEGEDKIAALKEVVGKYIDRRLAEILR
                     AKAEEK"
     gene            complement(20348..21484)
                     /locus_tag="VF226_03595"
     CDS             complement(20348..21484)
                     /locus_tag="VF226_03595"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX7073101.1"
                     /translation="MGKLLRALGLMSGTSMDGIDLALIETDGENQVARRQHGSCAYPP
                     EFRARLRAALADARLLTDRIARPGCLADVERALTERHAEAVSAFLSEHGIRAADVDVI
                     GFHGQTVLHRPAVPGDFGVPSPGLTVQLGDGGLLARLTGIDVVYDLRAADVAAGGQGA
                     PLAPVYHRALAARLPQRPVAFLNIGGVANVTWVGENGRLIAFDTGPGNALMDDWALRH
                     TGRACDVDGELARSGKADEEVLRQYLMHPYLERPVPKSLDRNDFTLDLVEGLSAADGA
                     ATLAELTAVAVARSLSWLPAPPALWLVTGGGRRNKHIMELLAWHLEAPVAPVEAVDLD
                     GDSMEAEAWAYLAVRSLASLPITYPETTGAPRPLTGGVLAPAPK"
     gene            21568..22830
                     /gene="tyrS"
                     /locus_tag="VF226_03600"
     CDS             21568..22830
                     /gene="tyrS"
                     /locus_tag="VF226_03600"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX7073102.1"
                     /translation="MPAPASDFLRILSERGFIHQCSDFEGLDALAARREVIAYVGYDC
                     TAPSLHVGHLLSIMMLHWLQQSGGGKPITLMGGGTTRVGDPSGRDETRRILSIEQIDA
                     NKESIKTVFSKFLKFGDGATDAIMVDNAEWLAPLNYIAFLRDVGRHFSVNRMLTMDSV
                     RLRLERQQELSFIEFNYMILQAYDFVELARRYGCNLQMGGSDQWGNIVMGIDLGRRMG
                     THQLYALTTPLLTTSSGAKMGKTAAGAVWLNEDQLSAYDYWQFWRNTEDGDVERFLKL
                     FTTLPLAEIKRLASLEGAEINEAKKVLATEATALIHGRAKAEAAAETARAIFEQGALA
                     AGLPTVDIPASTIDAGLGVLKAFVSAGLVKSTGEARRQIQGKGLRVNDEVVQDEKAQL
                     TRADLTAEGVIKLSLGKKRHVLLRPVRA"
     gene            complement(22874..>26098)
                     /locus_tag="VF226_03605"
     CDS             complement(22874..>26098)
                     /locus_tag="VF226_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX7073103.1"
                     /translation="IEPRLLLTYSQDGGLSFSFTRPAGSAGEGVPGGSDVQTLPTEQS
                     SGEGRDAGSGLPVALRQLGLARVIAEASARARQRMDATSFLRQIGLRDATLIVDHAGE
                     QKVWRVPQADVALSHKKLRSLIEGSVTVDSGGRPWTLQFSSEASGETEQVNLKASVRD
                     LIPSALADTFPALSPLHFLSMPASGDVNLQLTSQGKVLGGTFSLDLSRGDMYLPWAGD
                     LPLHFEGGSLDLRYDPAARKLRIEPSTVRWGKSRLTLEGEIASSFDQRGEEIWAFRLS
                     GVDGELAPEETDRAPVPVQEWLTEGTVLPEEGAFSLRQKLVAGGGELHLTVDVDGQEG
                     GRGARLEGQMGAMPVATLLGIWPRALGTDMRTWIRDSIPSGRVLGGSFRYIGGSEAAG
                     QAAPQRLSIAVQASELQMRPGSKLPPIAIPSALIRLEGSTLEVTVPEAAVALANGRSL
                     SLKAGRFIAVDLFSSHSTAELAFQGEGSVAALLELIRHESLGGVQLGGIQTEHIEGKV
                     EAALTIKLPLKSEITFADLSVEGQGRLTEARAPKLLGNHDAQAATVTFDITDKAIDAR
                     GDLLIAGVPVKLAWQHIFEAPPEKQPPLRLTATLDRTDRDQLDLDPGHLVQGEVPVEI
                     TITRSADEEHHVHVWADLTGADMVLDNVVWRKPPGRSAVMQFDIARGGRYALELQNLK
                     IVGDDIAISGWMGLDEKEKMREFHFPEFSANIITRLDVRGVLKENGVWDVKANGPTYD
                     GRDVFRALFSVGELSLDRPEERRHMPGLDLEAEIATVVGFSDVSLRNLKLKMSRRDGK
                     LTQLNARGNLDGGKPLDVELRRAPDQSRVLVAVTDDAGRAFRLVDFYPSLVGGRMRLE
                     VNLDGRGAAEKTGLLRVERFRILGDPIVSEVLQVPDETRPYVGQRRQRVVRQTFDFDW
                     MRVPFSLGHGQFVMGDSEIRGPLVGATLRGKADFRSRQVNIGGTYVPLQGLNSAIGFI
                     PGLGQILAGPKGEGILGITFAIRGPMAQPDVLVNPLSLVAPGIFREMFQMTPDQSVTP
                     PRTAPRGRSSGQAPGSSAASTAARRTGAVQSDVVSGWSSETRKN"
BASE COUNT         4550 a         8697 c         8114 g         4737 t
ORIGIN      
        1 gaggcgacga gccgcctgat cgaaagctgc tggaacacgg cggacctgaa agacctgcgt
       61 tcgctgacag cgctcgcggt tccggcatga taatcgcacg ggcgtctcgg gcaggccctt
      121 cttcatgctg gaccgtccag acttctagct ttaccaagcg aacttaagag gccgggctag
      181 acatatgctt ttttatgcgc tgtgttggcg gcagaacgac cttctcactc acgcgtgcct
      241 cacgataacg acgggggaac ttgatgccgt tgctcggaaa gggaatgctc atcacgtcga
      301 tggacgtcga tcccgccgaa gaagccgact tcaatctctg gtacgaccgc gagcatctgg
      361 cagagcgcgt tgccattgaa ggattcctgg aggctcggcg ctggatcgcc gacgaggccc
      421 cgaccaaata cttctgcacc tactcgacgg atacgttcga ggcgctgagc agccccgcct
      481 atcagaaggc cctcgccaac cagaccgact ggtcgaagca ccacatctcg cgcttccgca
      541 atcccggccg cgccgtcggc aggatcacgg tgagccgcgg ccagggacgc gggggcgtgc
      601 tcggagtggt gcgcctgcgg cctggcgcgg atgcggacaa ggccgcgttg cgtgagcgcc
      661 tgcgcgagct tctcgatccc ggaatgctgc cgggcatcat ctcgatgcac ctcgtcgaga
      721 gcgatccgac gctgtctcag aatctgaccg accccaacgc gcccaatccg ggcgccgcgg
      781 actggtacgt gctgatcgac ggctcagatc tcgccgccgt tcgcgccctc atgaacgacc
      841 ggttcagcca gccgggcgcg gagatcgtct ccaaggccac gtatcggctc ctttgggatc
      901 tcgccaagtc ggatctctga gccggtccat atcaccaaac atatcttcat cagactggag
      961 gactgtaagt gaacagaact gtgaaatctc tgctcgtggc cggcagcgtc gccgcgaccc
     1021 tcgttgcgag caccgccgcg ttcgcggagc ggtggaacat gccggtccgc tcgaatgagc
     1081 ggaactactt cacccgcaac atcatgcagt tcgccgacga ggtgaagaaa gcgacggacg
     1141 gcaagctcga gatcgtcgtt cacccggaag attcgctcat caagcagccg gaagtgaagc
     1201 gcgccgtgca gacgggccag gtgcagatcg gtgagcttct gatgtcgatg cactcgaacg
     1261 agaatgcgat cttcggcgtc gattccgttc ccttcatggc caagaacttc gaggaagggc
     1321 gcaagctgct gggcatcacg cgtccctacc tcgaaaatct cctgaagcag cagaacatgc
     1381 gcctgctgtt cgtcgtgccg tggccggcga actcgttcta cgtcgacaag gaggtgaact
     1441 cgacgaagga cttccagggc ctgaagttcc gtgcgttcaa cccggtcacc ggccgccttg
     1501 ccgagctgat gggggcgatc ccggtcacgg tccagcagtc cgaggtctcg caggcgttct
     1561 cgaccggcgt catccaggcg atgatcacgt caccggcgac cggcgtcgac acgcaggcct
     1621 gggatttcgt gaaatacttc tacgaagttc gggcgatgtc gccgtggaac atcgtcgtcg
     1681 tcaacgagcg catgttccgc cgcctcgacg agaagacgca gaaggccgtg ctcgacgcca
     1741 gcaaagccgc cgaggagcgc ggctgggagc ttgccgccag cgaaaccggc aagctgatcg
     1801 atacgcttaa ggagcatggg atggaagtcc gccagccctc gcctgagttg atggcggaac
     1861 tgagcaagat cggcgacacg ctcatcgacg agtggatcaa gagcgcgggc gccgacggcg
     1921 agaagatcat gaacgagttc cgcggcgaga actgatcgat tggcgtgatg ctgacggctt
     1981 tgaaccgaat ctacgcggcg ggcgcagtgc tcgccgcgtt gtgtatggtg gcgatcgcgc
     2041 tgctgacgct ggcccagatc atgggccggc tgtttggcgt gctgatcgtc gacgcgggtg
     2101 agctcgcggg tttcgcgatg gccggctcga tattcttcgc gctggcgcat acgctgcgca
     2161 ctggcggcca catccgggtc aatctgctgt tggcgcgaat gcggccgagc attcggcgca
     2221 tcttcgaaat ctggagcctt ggtctcgcaa ccaccatcag cggaatgttt gccgtctttt
     2281 ccgtacggat ggtgatcgag tcctacgaat ttcatgacgt ctccacgggc atgataccgg
     2341 tgcccctatg gattccgcag ctcagcatgg cagcaggtgc ggtgctgttc gtcgttgccc
     2401 tgctgcacga gctcatactg accattcgcg gccaggaggc aggctacgtg gcggctgaga
     2461 acactgagcc ctatacggag tagccggcgc atggacgcca ttttctacag ccctattctg
     2521 atcgtagcgc tgctcggcct tctcgccagt ggcgtctgga tcgcgatcac cctggcggga
     2581 gtggcaatga tcgccatgct gctcgtcgag gcgccgatcg atctcgtgat gccatcctcg
     2641 atctggagcg ccgtgagcag ctggtcgctg acggccctgc cgctcttcat ctggatgggc
     2701 gagatccttt tccgtacccg tctggctgac gatctcttcc atgggctcgc cccctgggtg
     2761 aaccgactgc cagggcgcct gctgcacgtc aacgtcatcg gttgcgggat ctttgcagca
     2821 gtgagcggat catcggcggc gacggcagcg acggtcggcc agatgtccct gccggagctg
     2881 aagcgtcgtg gttacgatga aaaactcagt ataggttcgc tggcatcctc cggaacgctt
     2941 ggcctactca ttccaccctc gatcatcctc atcgtctacg gcgtctcggc caacgtctcg
     3001 attgcgcgcc tgttcctcgc gggcgtcgtg cccggcctga tggtcgtcgc actcttcatg
     3061 gcctacatcg tcatctgggc gctgctcaat cgggagaaga tgccgccagc cgaggcgggc
     3121 ctgagcttct ggcagatggt cagccgtttg cggctcctgt tgccaacggt gcttctgatc
     3181 atcgcggtga tcggctcgat ctacacgggt gttgcgacgg caacggaggc ggcgactctc
     3241 ggcgtcgtcg gctcgctgct gattgcattg ctgtcgggaa gcctgaactg gcagacgttc
     3301 cggatgagcc tctacggcgc gacgcgcact tcgtgcatga tcggtttcat cattgcgacg
     3361 gctgccttcc tctccgtagc catgggctat gccgggatcc cccgcgcgct cgcttcatgg
     3421 attggcacct tcaacctgtc cgcctaccag ctgatcttcg cgctggccat cctctacatc
     3481 attctgggat gcttcctcga cggcatctcg atggtcgttc tgaccaccgc cgtcatcatg
     3541 cccatggtgc aggccgcgca tatcgacctg ctctggttcg gcatcttcat cgtgctggtg
     3601 gtcgagatcg cgcagcttac gccgcccgtc gggttcaatc tcttcgtcgt gcaggggttg
     3661 accgggcatg acattctcta cgtcgcccgt tcggtgctgc cgttcttcct gctgttgtgc
     3721 gccaccgtcg ccattctgac ggtcttccct gagatcgcca cttggttgcc gagaaccatg
     3781 tttagcgccc gctgattgcg gacaccctct cgatgttcga caggagccct cctcatggca
     3841 cagtctgacc ccacccagct cacggccagc cagcttgctg cggatatcag cgcacgccgc
     3901 ctttcgcccg tcgacgttgt cgacgcctat ctcgagcgga tcgctgccaa ggattcgaag
     3961 ctccactcct acgtacaggt ctatgcggaa gaggcgcggc tcgcggccga agcggccgat
     4021 cgcgccattc gcgccggcca cgcggtcggg ccgctgcacg gcgtgccgat cgccctcaag
     4081 gatctcgtcg agatcgaagg caaggtcacc accggcggct gcgcggtttg gcgtaaccgc
     4141 gtttcaacct acaccgccac tctcgccaag cgtctcatgg cgcaaggcat gatcgtgctt
     4201 ggcaagacgc acacggtcga gttcgcaatg ggtggctggg gaacgaacac gcatctcggc
     4261 acgccctgga atccatggga cctggagcgc cttcgtacgc ccggcggctc gagcgccggc
     4321 tcgggcgtgt ccgttgccgc cggcctcgcg ccttgggcag tcggtacgga caccggcggc
     4381 tcggtgcggc tgccggcatc ctggtgcggc atcaccggac tcaagaccac gatcgggcgt
     4441 gtcagcacct acggcgtgct gccgctcagc ccgacactcg atacaccagg tccgatgacg
     4501 cgcaccgtgg aggatgctgc cctgctctac accgtgatgc agggcgaaga cccgatggac
     4561 gttcgcaccc gcggcgtgcc ttacacggac ccgatgccga ccctgaaacg cggagtgcgc
     4621 ggcctgcgtc tggcccggat gccgcaatcc gaacgccagt tcgcatcgga tgaagttctg
     4681 gccgcgtacg acagatcgct cgacgagctt gcccgtctcg gcgcggaaat agtcgatgtc
     4741 accctgccgt tcgcgctcgc tgacgtggcg gccctcaacc tgcgcatcat ggcggcggaa
     4801 agctacgcga tcctgcatga gctgatcgac gacgagagca cccagctcga cccggacgtt
     4861 cgcccgagga tcgccgccgg ccgtagcgtc acggcgaagg aatacctgga agccctgcag
     4921 gcgaaagagg accgaaagcg ccagttcctg gcggcgctcg acggcatcga cgcactactc
     4981 acgcccacca cgatgacgac agcgattccc gtcgacgagg tcgatcagaa gacggcgccg
     5041 gcgcacttca cgcgcttcgg caatttcctc gaactctgtg gtgtcgcact gccgaacggc
     5101 ttcgacagca aggggctgcc gacgtcgctt cagatcgcct gccgtcccta tgacgaggcg
     5161 accgcgctgc gcatcggctg ggcctatcag aacgccaccg aatggcacct gcgccggcca
     5221 tcggtctaaa tgttccgtca cgggcgctta cttcctcctc cgtcatggct gcggaggcgg
     5281 ccatccacgc aagtttccgc atttgagcag cgaggtgcgt ggattcccgc ctccgcggga
     5341 atgacggcaa ttgtcggaat caggacacta gcgcgagagg tcaggggaaa tcgtgagtac
     5401 acgtgaccag ttcgtcggcc tcggcgagcg gctttatcgg gtcgagcgcc cctggggcaa
     5461 gctgccgagc catttgagct acgcggggat caccgacgtc accgtcttgc cgtcagggcg
     5521 cattgccgtt ctgctgcgca gcgatccggc ggttctcgtt ttcgaccccg acggcacgct
     5581 ggccgatcaa tggtcgttgc cagacgtcgt ggcgggacac tacatccgcg ccatgccgag
     5641 cgggcgcgtg cttctcgccg atttcgacgg ccatcgcatt ttcgtgctga gaggcgatga
     5701 cggccgctgc gagaagatcc tcggcgcccg cgaccgcccg cggtttggcc agcccttcaa
     5761 tcatccgacc gatgccgtcg aggcgccgga tggcgagctg tacgtcagcg atggatacgg
     5821 caactcctgt gtgcatcggt tctccgccga cggcacatgg atcgcaacct ggggcaagcc
     5881 gggcagcggc aagctcgagt tctccacccc gcatgcggtc gtcttcgacc gcgccggcag
     5941 gctgctcgtc ggcgatcgcg agaacaatcg cgtgcagatt ctttcgcgtg acggccaatg
     6001 gcaaggggag atcagcggcc tgttcaagcc gatggacgtc gagccgacgc cagacggtgg
     6061 cgttctcgtc accgatcaga cgccgcggct gtcttatttc tcggctgaag gcgttctcat
     6121 cggccgctgc cggaccttcg gcacggtagg acatggaata gcgctggcgc cggacgggtc
     6181 catcttcgtc gcagagatgg ggcccgatat gctcacgcgc tttgccccgg tcaccgtctg
     6241 actcgagcca ttatgcaggc aagactgact gccccggagg atgaccggag cagtgcgctt
     6301 tttgtgggct cgatttcgtc tcagaacccg acagcgatcg aaacgctcgg gccataggcg
     6361 taggcgtatg gggcactgta gaacccgagc ggccggcgat atcggtagta ggggccggga
     6421 ccatagccga cgatgcgcgg gccataaccc cagtagtagg gccgcgcagg aacatagtag
     6481 gacggcgcat agtatgagta tcggtagggg cgcaccggcg ccgaataata cgcggcgggc
     6541 cggtcacaac cccaccagcc gccggcgacc gccggagtgc cgccggcagc ggccaggagc
     6601 ggaatggcga gcgacaacgc tgcgagtgca ggagtacgca tcctcaatcc tccacggttc
     6661 aaccgatgct gagaacgtgc ctgcattcgt tcgtgttccg ccccgcgagc gagcatcgcg
     6721 gggccgcagc cgataagttg aaaaatggtg aacgggccag ctaggcctcc tgccggttgg
     6781 aaggcagcag tatcgtagag aggctctatt gggcatattg ctctaagagt tgcatactcg
     6841 gcctgagcct taccacctcg tcaggagccc gcgcgatgac cttcgacctc tcgtcgattc
     6901 tctggctgtt gctggtgttc gcggtgctgc aaccgctcct cgtcagccgc tggctgacga
     6961 tgcggcgcgc acaagccatc cgcgccatgg agaaggcgcg cggcacgcgg gtcatcacca
     7021 tgatccatcg gcaggagcgc cgcagcctgt tggggctgtc cgtctcccgc cacatcgaca
     7081 tggaggacgc gcagaccatc attgccgcca tcaaggagac gccgcccgac gtccccatcg
     7141 acctcgtgat ccacacaccg ggcggcctcg tgctcgcggc catgcaggtg gcacgtgcca
     7201 tcgaagcgca tccggcgaag gtcaccgttt tcgttcccgt gtacgcgatg tcgggtggca
     7261 cgctgatcgc gcttgcggcc gacgagatcg ttctcggaga gttctccgtg ctcggcccga
     7321 tcgaccccca gatcgccggg ttgccggcgg cgagcatcgt caaggcgagg gattcaaagc
     7381 cgccggaatc cagcttcgac ctgacgctcg tgctcgcgga tgtcgcagaa aaagccttgg
     7441 ctcaggtcaa gcgcggcgcc gtggagctgc tgaccccgcg cctcgaccag gcctcggcag
     7501 aggcgctggc cgacaagctc gctggcggcc attggaccca cgactacgcc ctcaccgcaa
     7561 cggaagcgca ggatctcggc ctgcccgtca aggtcggcat gcccaagacg gtgctggagc
     7621 tgatgaagct ctatccgcag ccgatcatgc cgtcctctgt cgaatttctc ccgatcgatg
     7681 tgccgcagaa acggcgcatc tgacaaagcc ggctaatccg ggcacgggaa actgaagctc
     7741 acccagggcg tgcggatcgc gcactcctga ggcttggagg cgcttcgcgc gggctggtcc
     7801 gacttcggct cggttttcga tgcagtggag ctccgaggaa ccgtcttgcc gtccgcggca
     7861 gcggatttgg tttcagcacc cgttttcgac tcgactttgg atttgtttcc ctcaggcgat
     7921 gcagcggtgc cgttcgacgg actgatcgca gtccctgccg ccgccggcgt gcctgcgtct
     7981 tcattgggag acagggcggc cgtggtgccg atatctccgg cgcaacgctg caggtcggca
     8041 ggcgcgcgcg tccacgtcat cgtttcgctg agcagcttcg tcccgagata gcccgttacc
     8101 tgcagcttct cctccccgat aggcttcagc tccacgctga acctggcgtc ctgttccgga
     8161 tcgtaaatcc agcccttgtc ccagacgcct gcgccaaccg gcttcacatc gccgaggatc
     8221 tggatgccgc acgcctctgc gttctccgta tccttcagcc agacaacccg cccgcacagc
     8281 gcgccattac agtcggtgat ctccacggcg ccgcggccgg tgtggtcgat ccagacgcct
     8341 ttcggatcgg ttgccgcatt cgccccgacc gcacacagca aggtggcagc cgctgcggcc
     8401 gtggtcagaa atcgtcgatg aatgtgcatg ctgcacctct catgattccg cttctcagct
     8461 ccaatgctcc agcccgctga tgggcggacg gtctggcgag atcttgtatt gcctaggcca
     8521 ccagagctgt ttcgtgggag acagttacgg gcagagtgtg gccgtcagat gccgcaccgg
     8581 aggctgcgac tccgatgcgc ggcttaaagg cgcatgtttc tcttgcgttt cgaacccatg
     8641 tatgtcagca ggagcctgtg gtgagcgcag gggatcgcat tcattagtcc ctttctcacg
     8701 cacgcttttc ttgttttgcc actttggcat cccgtatccc cctgcgctcg atcggtcgcc
     8761 gctctcgctt gagaggtgcg cggccctatg tgaacgcaga cgaggaacga ggtttttttg
     8821 ccgacgaaga cattcgctga gctcgggctc agccaaaagg ttctggctgc cgtagaggct
     8881 gccggttaca catcaccaac tcccatccag gccgaagcaa tcccggtcgc attgacgggt
     8941 caggacgtgc tcggaattgc ccagacgggc accggcaaga cggcatcgtt cgttctgccg
     9001 atgattaccc gcttggaatc gggccgcgca cgggcgcgaa tgccgcgctc gctcattctg
     9061 gccccgaccc gcgagctggc cgctcaggtc gcccaaagct tcgaaaaata cggcatcaat
     9121 cagaagctca ccgtcgccct gctgatcggc ggcgtgtcga tggacgatca gacccgcaag
     9181 ctcgaccgcg gtgtcgacgt gctgatcgcc acaccggggc gcctcctcga ccatttccag
     9241 cgcggccgcg tgatgctgat gggagtcgag atcctcgtca tcgacgaggc tgatcgcatg
     9301 ctcgacatgg ggttcatccc ggatatcgag aaaatctgca agctgctgcc gccgaagcgg
     9361 cagacccttt tcttctccgc caccatgccg cctgagatcc agcggctggt cgatcacttc
     9421 ctcaccaatc ccgcccgcat tgaggtggcc cccccggcga cgacggtcaa agccatcacc
     9481 cagcgcttcg tgttctgccg cgacagcgag gactgggcaa agcgcgaaca acttcgccag
     9541 ctcatacgca gccaggacgt ccgcaacgcc atcatcttct gcaaccgcaa gcgcgacgtc
     9601 gccatcctga acaagtcatt gctcaagcac ggcttcaacg ccggcgcgct ccatggcgac
     9661 atggatcaga agagccggat ggagacgctc gacgcattcc gctcgggcga gatcaccatt
     9721 ctggctgcga gcgacgtggc ggcacgcggc ctcgatattc ccgaggtgag ccacgtcttc
     9781 aacttcgatg tgccgtgggc cgctgacgac tacgtgcacc gcatcgggcg cacgggccgc
     9841 gcgggccggg aaggctacgc ggccacgttg gtgacgcgcg aagacctcaa gcttgtgaag
     9901 cagatcgagc aggtcactgg cgagcagccg acctggatcg gcgagccgcc gacggaggag
     9961 gactacgccg aagctgctcg gaagcgccgc gggcgccgca acggcaaatc cgacgcgcgg
    10021 cgcgccaatg gtcgggccga gcccaagccg aagcgtgaac ggcagcggcg cgagaaatca
    10081 gcaccggcac aacccgctgc ggaggcgcaa ccgccgcaga aggaggcagc cgagcctctc
    10141 gagactgcgc cccgtcgcga aaaaccccgg acgaagccgc gggccgtcga aacgccacag
    10201 cccgcaccgg agcaccgcac ccgccagccg cgggagcgca gaccggagtc agcaaacgtc
    10261 gggttcgccg acaagatccc ggccttcatg cgtaagccgg tccgggtcgc aaaggcggcg
    10321 aacgagtaag catccgctgc ttgggacgcc tcctcacttc attcgagcaa ttcgagcaga
    10381 gcttccagcc gaagtgccca tgcgcttcgg ctttgcccgt tgtgcccgac gggccgcggc
    10441 cagggcgccg cgggcccagg cgacgagctc gtcaggatcg tcgagcaggc ggtccggcgc
    10501 gcgccgatac gaggagatcg tggtgcggcc ggtctttgtg ccgtagctga acggcggcag
    10561 tgactcggca tcgaagcaac cgccgttggc ttcgtccgtc ttgaaataga gcgtgtcgtc
    10621 cgaaaccagc gcgaacatca cgccgtcgga ataaaggccg gcgccgccga acatgcgacg
    10681 gacttggatc tgtccgagcc cgctcagctg gtcacgcagc agctcaagaa attcggcgct
    10741 gctgcccatg ccaaatctct cggtcagaat gagctgccgg gctgtttgag gaattccact
    10801 tcctctgggg tcgattggcg gcccagcacg gcattgcggt gcggaaagcg gccgaaccgc
    10861 gcgatgatgt cgcgatgcgc aatggcgtag cgcgtgtact cgggatcgcc gagaggcgtg
    10921 aaaagctcca cagcccgctc ctgatcggcc agatcctcgc tgtgctcgaa cggcaggtac
    10981 atgaacgcgc ggccatcctt gtcgaaggct ttatcgaacc cgcgctcgac ggctccgcgc
    11041 gccacttcaa ctgccttgcc atccgttgcg aacgcccgcg gcgtcccacg gaacatattt
    11101 ctcggaaact gatcgaggac gatgatcgtg gcgagcgcac gccgcgggct cgtccaggcc
    11161 gcatcagccg gcaaggccgc gacccgctcg tgcacattca tgaaacggct gcggatgagc
    11221 tcgtccgtgg cgtcgctctt tcggaaccag tcctcgtgcg gcagctcctc gaaccagaat
    11281 ttcaggacct cctcgaccca ggcctcctcg cctccggcgc tggcctcagt gtcacgggca
    11341 gtgtcggtca tttagaggct ccggtcgcgc ctgagatttc gaagaaagcg gattgcgccg
    11401 agataggcag gtcagcccct gaccgtgtcg aggctttcct tggaagcggg cgtaatggtg
    11461 accgacactc cgcagccgca ggcgctggac tggttgggat tgttgaagac gaactgcgag
    11521 gaaagcttgt cgaccttgta gtccatctcg gttcccagca ggaacaggat cgccttgggg
    11581 tcgatcagga tcctgacgcc cttgtcctcc accacctcgt cgagcttgtc ctgctcggtg
    11641 gcccagctca tcgtatattc catgccggcg cagccgccgt tcttcacgcc gacgcgtaac
    11701 cccaccacgt ccgggcggga ggccatgatc tgacgcacgc gctcagcggc ggcgtccgtc
    11761 agggtcatca ccttgggcct gggtcttgca gttgccatcg tctcaccatc caacggttca
    11821 aggtcgtgga tgctctcaat atagattgct tcgccgcctt tgcgagcggc gcccgcggct
    11881 caagactctc agaacatgtt gatcgccaac cgcgcctcgt cgctcattcg gctcatatcc
    11941 caggggggat cgaacgtgag ggtcacggtg cagccactga cgcccgggac cgagcacacg
    12001 gcattctcca cccaggtcgg catttcgccg gccaccgggc aacccggcgc cgtcagcgtc
    12061 atctcgatgc tgacgttgcg gttgtcgtcg acgtcgatct tgtagatgag ccccagctcg
    12121 tagatgtcgg ccggaatctc ggggtcgtag actgtcttca gcgccccgat gatgtcggca
    12181 gtcagttgct ccagctcctc ggacgagaga gcgctgccct cgaccggcgt ccctcccgcc
    12241 gcgacactcg gcgccgagcg tcgctccgta tcgggctccg ctctctcgga cggagattcc
    12301 gaaatgaatt caagcacgtc ccgctcacgg tgcttctcgt ccatatcgca tcaactcctc
    12361 tggcgctcag gcgaagaact cgtgagcgcg aaccaaggcg gccgctagcg catcgacgtc
    12421 ggccttcgtg ttgtaaagcc cgaaagaggc ccggcacagc gccggcacgc caaagcggtc
    12481 catcagtggc tgcgcgcagt ggtggccggc acgaatggcg atccccgagc ggtcgacgat
    12541 cgtggagacg tcgtgcggat ggaggccctc gacgctgaac gatacgatcg cccccttgtc
    12601 cggcgcggtg ccgaagatct tgagccagtt gtagtcgccg agccgctcgt gggcgtactt
    12661 gacgagctcg ctctcgtagg cggctatttc atcccggccg atctgcatca tgtagttgag
    12721 cgccgcgccg agcccgatcg cctcgacgat cggcggcgtg ccggcctcga acttgttcgg
    12781 cagctggccg tacgtgatgc cgtccaccgt gacgctctcg atcatcgacc cgccgccgag
    12841 gaacggcggc agagcctcga gcagcgcctt gcggccccag agcacgccga tgccggacgg
    12901 cccatagacc ttgtggccag taaatgcgta gaaatcgcag ccgatgtcct ggacatcaac
    12961 gggcagatgg accgccccct gagagccgtc gatcagcacc ggtataccgc gctcgcgcgc
    13021 gatccggcag acctcattga ccggtacaac ggttccgagc acgttcgaaa cgtgcgtcac
    13081 cgctacgatc ttcgtgcgcg gactgagcag ccgctcgaac tcgtcgagca ggaactcgcc
    13141 cctgtccgag atcggcaccc acttcagcac cgcgcccttg cgctcgcgca gatagttcca
    13201 ggggacgatg ttcgagtggt gctccatgat cgagagcacg atctcgtcgc cttcctggat
    13261 gttcgcgagg ccgaaggaat acgcgaccag gttgatcgac tcggtcgcgt tcttcgtgaa
    13321 aacgatctcc tccgcagacg gcgcattgag gaagcggcga accgtttcgc gcgcatcctc
    13381 gtatttctgg gtcgccgtat tggagaggag gtggatgccg cgatgcacgt ttgcgtattc
    13441 gaagcgcagc gtgtgatcca tcgcctcgat caccgacagc ggcttctgcg ccgatgccgc
    13501 gctgtcgagg tatacgagcg gtttgccgta gacctcgcgg aacaggatcg ggaaatcggc
    13561 gcgatagcgc tcgacgtcga accgcctgcc ggtcgcagtt gccgctgtct gtgtcacacc
    13621 tgatccggcc cttatgaagc cgtcctctgg ttcagccacc ggcgcgccgt ttccatcagc
    13681 gcgtcgcgca ctgcctcgtc ctcgaccttg tccatggctt ccccgacgaa ggcctcgatg
    13741 aggagggccc gcgcctctgg ctcgggaatg ccgcgcgacc tgcaatagaa aatatgatcc
    13801 ggatcgattt gcgccgaggt ggagccgtgg ccgcagatca cgtcgtcggc gtagatctcg
    13861 agctcgggct tgctgtcgaa ctccgcatcc ggcgacagca tcagcgcctg cgccatctgc
    13921 ttgccgtcgg tcttctgcgc gccgggctgc acgatgacct tgccctggaa aatgccgcgt
    13981 ccacgcccgt ccaacacgcc cttgtagagc tcgcggctct cgcagtgcgg caccgcatga
    14041 tccaccacca gcgtcgtgtc gatatgctcg ttgttcgccg ccaggaaggc gcccgagcaa
    14101 tcgagcttgc tgccctcgcc attgaacgtg acgaagatct ggttgcgcgc aagccccgga
    14161 ccagcggtga gctggaaggc acgataactc gcctcggcgc cgatttcggc cgtggcgctg
    14221 ccgagatgca ccgccttgcc gttctcgatg atggccttga tgtgcgccac cgccgcgccg
    14281 tcgccgacgg cgatttcggt cgccgcgttg tgctgatcct cgtcagccgc gccgttgagc
    14341 ggcacgaaag cctcgatcag cgtcaccttc gcacccttgc cgatgtcgac gacattgcgc
    14401 gtcgttaccg acttgcggtc tttgccagcg cgcgcaaaga cgagcagcaa cggcttgtcg
    14461 agcacggtgt tgtcggcgac ccgcaccacg gcaccatccg tcgcaaaggc cgagttgagt
    14521 gcctcgatcg cgtccgtgtc acgcttcata cggacgaggt tttcggcctt ttcgggcgac
    14581 tcggccaggg cctcgctcag cggcttgacc gtcaggccgt ccgggagctg tttggccgaa
    14641 aggcccgcat cgaaggcgcc gtcgacgaca aggattcggt acgcctcgat ctcggccagc
    14701 ggcccgagtg cagcattgat gtccgcctcg ctcagcggcg ccggcctccc ggcggccggc
    14761 gcgaacgctt ccttcaggaa gctgcggaga tccgtatact tccactcctc gatgcgcctg
    14821 tgcggaagcc cgagcgcagc aaaagtgccg atcgcctggc gacgcagctc gcggacggcg
    14881 ccgctgcccg gaagccgggc gacgaccgca tcgaaggttt ccgtgaaggc ctgctcggcc
    14941 ttcgtttttg tcaagcccac gttcatctcg tcaacacccg ctcagcctta cgccgccttc
    15001 gtgaactcgg catagccgga ccgctcgagc tccacggcca gctccttgtc gcccgtccgc
    15061 tggatgcggc cgtgcgccag gacgtgcacc ttgtcgggca cgatgtagtt cagcaggcgc
    15121 tgatagtgcg tgatgaccag catcgcccgg tccgggctgc gcagcgcatt gacgccgtcc
    15181 gccacgatcc ggagcgcgtc gatatcgagg cccgagtctg tttcgtcgag cacgcacatg
    15241 gtcggctcaa gtaacgccat ctgcaggatc tcggagcgct tcttctcgcc gccggagaag
    15301 ccgacgttga cggggcgctt cagcatctcg acgtcgatgt tgagcttggc cgcacgatcg
    15361 cgcacgagct tcatgaactc cggcgtcgtc agctcggcct ggtcgcgctt cttgcgcagc
    15421 gcgttgagcg ccgtccgcag gaacgtgaga ccggcgaccc ctgggatctc catcggatac
    15481 tggaacgcca ggaacacgcc cttggcggcg cgctcgtcgg gctccatctc gaggaggctt
    15541 tcgccattcc acaggatgtc gccttcggtc acctcgtagt cgtcgcgacc ggcaagaaca
    15601 taggcgagcg tggacttgcc cgagccgttg gggcccatga tcgcgtgcac ttcacctttg
    15661 ggcacgacga ggtccacacc tttgaggatc tcggtgtcct cgtccgcgat cttcacatgc
    15721 aagttccgga tctcaagcat ttccacgtct ctccttcggg cggcgcattg ccggcccatt
    15781 ttcgctactg gatccgactc acgcgtaggc ggagccggta tcctttccat tgccttgctg
    15841 cgccatccag tagcggtcga aggcggcgct cgccgtccgc tccgcttcct cctggcgcgc
    15901 cttgagctgc tcgatcgatg cgacgagcac gctgacgtcg ttgccatccc ctccaagggc
    15961 ctgctgcatc cgcgccagct ccgcatgggc cttgagggcc tcctgcttca gggcatcggc
    16021 tgctgtttcg gcctgcagcc aagcctgttc gagctgggcg acatcgctgg acatttccgc
    16081 ttcctccact gaactccagg gcctcggctc tcgtccgtat cgccccgacg cttgccctct
    16141 ttcgtccacg tgccccacgt cactcggctg ccggcccagg cacggggccg gcagcccttc
    16201 tcgtccgtca gccgacagag ccttcaaggc tgatgccgat cagcttctga gtctccgcca
    16261 tgaactccat cggcagctgc tgaatgacgt cgcgcacgaa gccgttgacg atgagcgcca
    16321 cggcctcctc ctccgacagg ccccgctgca tgcagtagaa gagctggttg tcggagatct
    16381 tcgacgtcgt cgcctcgtgc tcgaagtgtg ccgtcgagtt cttggcctcg atgtacggca
    16441 ccgtgtgcgc gccgcacttg tcgccgatca gcaagctgtc gcagttggtg aagttccgcg
    16501 cattcgtcgc cttgcggtgt gccgagacga gcccgcgata ggtgttctgc gagtagccgg
    16561 ccgcgatgcc cttcgagatg atccggctcg acgtgttctt gccgaggtgg atcatcttcg
    16621 tgccgctgtc gacctgctgg tagccgttcg aaatggcgat cgagtagaac tcgccgcgcg
    16681 agttgtcccc gcgcaggatg cagctcgggt acttccaggt gatggcagac ccggtctcca
    16741 cctgcgtcca ggagatcttg gcattgcggc cgcggcagtc gccacgcttg gtgacgaagt
    16801 tgtagacgcc gcccttgccg tccttgtcgc ccggatacca gttctgcacc gtcgagtact
    16861 tgatctccgc gtcatcgagc gcgatcagct cgacgacggc cgcgtgaagc tggttctcgt
    16921 ctcgcgtcgg cgcggtgcag ccctcgagat aggagacgta cgcgcccttg tcggcgatga
    16981 tcagcgtccg ctcgaactgc cccgtctcct gcgcattgat gcggaaatag gtggaaagct
    17041 ccatggggca gcgcacgccc tccggcacgt agacgaacga gccgtcggag aacaccgccg
    17101 agttcagcgc cgcgtagaaa ttgtcggact gcggcaccac cgagcccaga tacttcttca
    17161 cgagctcagg atattcgcgg atggcttccg agatcggaca gaagatcacg cccgccttgg
    17221 caagctgctc cttgaacgtg gtgaccacgg aaacactgtc gaacacggca tcgacggcca
    17281 ccttcggcgc tccctgaacg ccagccagga cctcctgctc cttgagcgga atgccgagct
    17341 tctcatacgt gcgcagcagc tccggatcga cctcgtcgag gctcttcgga ccctccatgc
    17401 tcttcggcgc cgcgtagtaa tggatgtcct cgaagtcgat cttggggtag cggaccttgg
    17461 cccatgtcgg ctccgtcatg gtgcgccagc ggcggtaggc ttcgagccgc cactcgagca
    17521 tccactccgg ttcgcccttc ttgcccgaga tgaagcggat gatgtcctcg ttgaggccct
    17581 tcggggccat caccatctcg atcttcgtct cgaaaccgta cttgtactgg tcgacgtcga
    17641 tcttccggac ctgctcgatc gtctcctgga ctgccattat cttctccgta cgaacccgtt
    17701 cttggatacc gactatctca ggccacagct cgttcccggc cgccgtgccc gacaatgctg
    17761 ccccacgccg ccaggaactc gtccacgtcg gcctcggtcg tcgtccaccc gaggctcacc
    17821 cggatggccg accgcgccac atcttccggc aagcccatcg ccgtgagcac cgggctcacg
    17881 ccgaccttgc ccgatgagca cgctgccccc gcgctgatgg caacgcccgc caagtcgaac
    17941 cggatcacca gcgtctcggc cgacaggccc ggaagcgcga gacacgtcgt gttgggcagg
    18001 cgctctgccc ccgacccgat tgccagcgca tccggcgcca tggccaaggc tccagactcc
    18061 agccggtccc gcagcgcccc gatccggccc atgtccgcaa gatcccgttt ggcggctgac
    18121 gccgccgcgc cgaaacctgc gatgcccggc acgttctcgg tgccggcacg ccgccgacgt
    18181 tcctgcccgc cgccgacgac gaacggtgcg atgtcgaaac cttccctcag aacgagagcg
    18241 ccgacgccct tcggaccgcc gagcttgtgt gcggaaaggc tcaacgtatc gaccccaagg
    18301 gtctcaaaat ccagctccat ccggcccgcc gcctggacgg catccgaatg gacgcaaact
    18361 ccgtgctcat gcgcgaccgc agcggcttcg gcaacgggct gaatgacccc ggtttcatta
    18421 ttggcaagct gcagggcaag aaccgacttg cccgagccac ttcgatcaag agctgacgaa
    18481 atggtcggca gctcggtcct gccgttggca tcgacaggga cttcgacgat agcagcgccg
    18541 ctggctcgcg ccggggccag cacggaatcg tgctctatgc cggcgaccag gatcgactcc
    18601 cacccggcgc gcaggaccgc gcagttggct tccgtcgctc cgctcgtgaa gaccacttgc
    18661 gccggagtga cgccgaccag cgcagccacg cgctcgcgcg cctcctccac gatcgcccga
    18721 gcgcgccggc cctcagcatg caccgaggaa gggttgccga gcgtatcgag cgcatcgagc
    18781 atcgccgccc gcgcctcggg ccgcagcggt gccgtcgcgt tgtggtcgag atatgtgcgc
    18841 gcaaaggtca ttgggctgcg gcgtccatct tgcttgcagg cagcggcgca cgcttttccg
    18901 gaggcacccc gtcgagcacc tcgcggaggc tcacgttcga aagaaagacc cggatgtggt
    18961 cgccgagcgc gtgccagaga ttatgggcaa tgcacttttc ttcgccgatg cagccgacgt
    19021 catcatcgcc catgcagcgc gtcatgcgcg tttcttcctc aacggcatgc atgatctcgg
    19081 cgaccgtaat ttcggaggcc agccggccca atctgtagcc gcccgcgcga ccacgcgcgc
    19141 tctccaccag acccgcgcgc ctcagcttga gaaagatctg ctcgagatag gcgatggaca
    19201 ggtgctgacg ctcggcaatt gccgacagcg gcaccgcacc ctcgccctcg tgactggcga
    19261 ggtcggccat tgccatcacg gcataccgtc ctcgcgtgtt cagctccact gacgcactcc
    19321 gcccgacaaa aaccaccggc tcgatagtct gattgccgcg acacgcaggt gttgcaatga
    19381 tcaacggcag tcgtgctaga agggcgagcc cgcagacctg ctccaccggc gtgtccgcgg
    19441 atctttgaac ctttctaatc tggccggact atgggaattc cgagcaccga agtcaagttt
    19501 taatgaaggg ctgatgcgcg acaccgtgac gagagctgta cgtgctgagc ttggttcccc
    19561 agataaaaga tcgagagtga cggcaattta gcgaggaagc tcgaaacaga aatgcctgag
    19621 ttgatcatca acggccctgc cggccgactc gaagcccgct atcaccacga gccggcgcgc
    19681 gatagcccga tcgcgctgat cctgcatccc cacccgcaat tcggcgggac gatgaacaat
    19741 caggtggtct atcatcttta ttatacgttt gccgaacgcg gcttctcggt gctgcggttc
    19801 aacttccgcg gcgtcggacg cagccaaggc ttcttcgaca acgggccggg tgagctggct
    19861 gacgccgctt ccgctctcga ttggctgcag atggcgaagc cggaggcgaa gagctgctgg
    19921 atcgccggtg tgtcgttcgg cacctggatc gcgatgcagc tcttgatgcg gcggccggaa
    19981 atcgatgcct tcatttgcgt cgcgccgcct gcgaacctct acgacttctc gttcctcgcc
    20041 ccctgccctg cgtccggcct cctcgtcaac ggcgagcggg acagggtcgt gccatcggcg
    20101 gccgttgcag agctcgcggc caagacgaag acccagagag ggatcaagct cgagcacgag
    20161 atcatccccg gcgccaacca cttcttcgaa ggcgaggata agatcgcggc actcaaggag
    20221 gtcgtcggca agtacatcga ccgccgcctc gccgagatcc tgcgcgcgaa agccgaggag
    20281 aagtagagag taagtcagcc gctctgggcc gctagcgccg cacacgattc gcggtgctgg
    20341 tcggcagtca tttcggcgcg ggtgccagca cgccaccggt cagcggccgc ggggcgccgg
    20401 tcgtctccgg ataagtgatc ggcaagctcg cgagggaacg gacggccaga taggcccatg
    20461 cctcggcctc catgctgtca ccgtcgagat cgacggcctc cacgggggcg acaggcgcct
    20521 cgaggtgcca ggcgagcagc tccatgatgt gcttgttgcg ccggccgccg ccagtcacga
    20581 gccacagggc cggcggagcc gggagccagc tcaggctgcg cgcgacagcc accgcggtca
    20641 gctccgcaag cgttgcggca ccgtcggcgg cagaaagtcc ctcgaccagg tccagcgtga
    20701 aatcattccg atccagggat ttcggcacgg gccgctccag gtaagggtgc atcaggtatt
    20761 gccgcagcac ctcctcgtcg gctttgcccg agcgggccag ctcgccgtcg acgtcacagg
    20821 cccggccagt gtggcgcaac gcccagtcgt ccatcagcgc gtttcctgga ccggtatcga
    20881 acgcgatgag gcgcccgttc tccccgaccc acgtgacgtt ggcgacccca ccgatattga
    20941 ggaaggcgac ggggcgctgc ggcaatcgcg ccgccagcgc acgatgatat actggcgcga
    21001 gcggcgcccc ctgcccgcct gctgcgacat cggccgcgcg aaggtcataa accacgtcga
    21061 tgccggtcag gcgtgcgagc agaccaccat cgccgagctg gacggtcaac ccgggcgaag
    21121 gcactccaaa atcacccggc acggctggcc gatggaggac ggtctggccg tggaagccga
    21181 ttacgtccac atccgccgcc cggatgccat gttcggacag aaacgccgaa acggcctcgg
    21241 catgccgttc ggtcaacgca cgctccacgt cggccagaca gccaggccgg gcgatccggt
    21301 ccgtcagcag gcgcgcgtcc gcgagagcgg cacgcaagcg ggccctgaac tccggcggat
    21361 aggcgcacga gccgtgctgg cgccgggcga cctgattctc gccgtcggtc tcgatcagcg
    21421 cgagatcaat tccgtccatc gacgtgccgc tcatgaggcc caacgctcgt aggagcttgc
    21481 ccatccgcac cttcccgtgc atgttcccgc gtcaaccgcc atcgtaaagc gaaactcgag
    21541 acgccgatac ccggcgcacc aggtcccatg cccgcacccg cttccgattt cctgcgaatc
    21601 ctctccgagc gcggcttcat tcaccagtgc tccgacttcg aggggctgga tgcccttgcc
    21661 gccaggcgcg aggtgatcgc ctatgtcggc tatgactgca cggcgccgtc gctgcacgtc
    21721 gggcacctgc tctcgatcat gatgctccac tggctgcagc agtcgggcgg gggtaagccg
    21781 atcacgctga tgggcggcgg cacgacacga gtcggcgacc cgtctggtcg cgacgaaacg
    21841 cgccgcatcc tctcgatcga gcagatcgat gccaacaagg agagcatcaa gaccgtcttc
    21901 tcgaagttcc tcaagttcgg agacggggcg accgacgcca tcatggtcga caacgccgaa
    21961 tggctggcgc cgctcaacta cattgcattt ctgcgcgatg tcggccgcca tttctcggtc
    22021 aatcgcatgc tgacgatgga ctcggtgcgg ctcaggctgg agcgccagca ggagctgtcg
    22081 ttcatcgaat tcaactacat gatcctgcag gcctatgact tcgtagaatt ggcccgacgc
    22141 tacggctgca acctgcagat gggcggctcg gatcagtggg gcaacatcgt catgggcatc
    22201 gatctgggcc gccgcatggg tacgcatcag ctctatgcgc tcaccactcc gctgctgacg
    22261 acctcctccg gtgccaagat gggcaagacc gctgctggcg ccgtctggct caacgaggac
    22321 cagctcagcg cttacgacta ctggcagttc tggcgcaata cggaggatgg cgacgtcgag
    22381 cggttcctga agctcttcac gacgctgccg ctcgctgaga tcaagcggct ggcgtcgctc
    22441 gagggcgccg agatcaacga ggccaagaag gtgctggcca ccgaggccac ggccctgatc
    22501 catggccgcg cgaaggccga ggccgctgcg gaaacagcgc gcgcgatctt cgagcagggc
    22561 gccctcgcgg ccggattgcc gaccgtggac atccctgcga gcacgatcga tgccgggctg
    22621 ggcgtgctga aggcgttcgt cagcgccggg ctcgtcaaaa gcaccggcga ggcgcgacgg
    22681 cagatccagg gtaagggcct gcgggtcaac gatgaagtcg tccaggatga gaaggcgcaa
    22741 ctcacgcgcg ccgatttgac cgccgaaggc gtcatcaagc tctcactcgg gaagaagcgc
    22801 cacgtgctgc tacggcccgt ccgagcctga gcttgcgggc gttcgcgcgg cgcaagcatt
    22861 gcccccgagc cggttagttc ttcctggtct cggaggacca gccggaaacg acatcggact
    22921 ggacggcacc cgtgcggcgg gcggcagtcg acgcagctga tgaacccggt gcctggcctg
    22981 acgagcgacc ccggggagcc gtgcgaggcg gcgtcacgct ctgatccggg gtcatctgaa
    23041 acatttcgcg gaagatgccc ggtgcaacga gcgacagcgg gttcaccagc acgtccggct
    23101 gtgccatggg acccctgatc gcgaacgtga ttccgagaat tccctccccc ttcgggccgg
    23161 ccaggatctg accgagaccc gggatgaagc caatggcgga attgagtccc tgcagcggaa
    23221 cgtaagtgcc gccgatgttc acctgccggg agcggaagtc ggccttgccg cgcagcgtcg
    23281 cgccgaccag cggtcctctg atctccgagt cgcccatcac gaactgtcca tgacccaggg
    23341 agaacgggac acgcatccag tcgaagtcga aggtctggcg aaccacgcgc tgacgccgct
    23401 gaccaacata gggccgcgtt tcatccggga cctgcaacac ttcgctgacg atcggatcgc
    23461 cgagaatgcg gaagcgctcg accctcagca ggcccgtttt ttcagccgcc ccgcgcccat
    23521 cgagattgac ctccagccgc atacggccgc cgacgaggct cgggtagaaa tcgacgagcc
    23581 ggaacgcccg gccggcatca tccgtcaccg ccaccaggac gcgtgactga tcgggtgccc
    23641 ggcgcagctc gacgtcgagc ggtttgccgc cgtccagatt accgcgggcg ttgagctgcg
    23701 tcagcttgcc gtcgcgccgc gacattttca gtttcaggtt ccgcagcgag acatccgaga
    23761 agccgacgac cgtcgcgatt tccgcctcga gatcgaggcc gggcatgtgc ctgcgctcct
    23821 cgggtcgatc caggctcagc tccccgacgg agaagagcgc gcgaaaaacg tccctgccgt
    23881 cataggtcgg accgttagct ttgacgtccc agacgccgtt ctccttcaga acaccacgga
    23941 catcgaggcg cgtgatgatg ttcgccgaga actccgggaa atgaaactcc cgcatcttct
    24001 ccttctcgtc gagccccatc caaccgctga ttgcgatgtc gtcgccgacg atcttgaggt
    24061 tctgcagctc cagcgcataa cgcccgcccc gcgcgatgtc gaactgcatg acggccgatc
    24121 gccccggcgg cttccgccag accacgttgt cgaggaccat gtccgcgccg gtcaggtccg
    24181 cccagacgtg aacatgatgc tcctcatcgg cgctcctggt gatcgtgatt tcgacgggca
    24241 cctcgccctg gaccagatgg cccggatcga ggtcgagctg atcccggtcg gtccggtcca
    24301 gcgtggccgt cagccggagc ggtggctgct tctccggagg ggcttcgaaa atgtgctgcc
    24361 aggcaagctt caccggcacg ccggcgatca aaaggtcgcc gcgggcatcg atggccttgt
    24421 ccgtgatgtc gaacgtcacg gtggccgctt gtgcatcatg gttgccgagc agcttcggcg
    24481 cgcgcgcctc ggtcagccgc ccctggccct cgacgctgag atctgcgaac gtgatctcgc
    24541 ttttcagcgg gagcttgatc gtcagtgcag cttcgacctt gccttcgatg tgctcggtct
    24601 ggatgccgcc gagctgcacc ccgccaagtg actcgtgccg aatcagttct aacagagccg
    24661 ccaccgagcc ctcgccctgg aacgccagct cggcggtcga atgactcgag aagagatcaa
    24721 cggcaataaa tcgaccagcc ttcagagaaa gactgcggcc gttcgcaagt gcaactgctg
    24781 cctccggaac ggtgacctcc agcgtcgacc cctcgaggcg gatcagtgca ctggggattg
    24841 cgatcggcgg caatttggag cccgggcgca tctgaagctc cgaggcctgc actgcgatcg
    24901 acaaacgctg gggagcagct tggccggcgg cctcagaacc gccgatgtat ctgaagctgc
    24961 caccgagcac ccggccgctc ggaatgctgt cgcggatcca ggtgcgcatg tcggtgccga
    25021 gcgcacgcgg ccagatgccg aggagggttg cgacaggcat ggcgcccatc tggccctcga
    25081 gtcgcgcgcc tctgccgcct tcctgcccgt cgacgtccac ggtgagatga agctcgccac
    25141 cccctgcgac cagcttctga cggagagaga atgcgccctc ctccggcaac actgtgcctt
    25201 ccgtgagcca ctcctggacg ggaaccggag cccgatcggt ctcctcgggc gcgagctcgc
    25261 cgtccactcc agacaaccgg aatgcccaga tttcctcgcc gcgctgatcg aatgagcttg
    25321 cgatctctcc ctcgagcgtc aggcgactct tgccccacct gaccgtggaa ggctcgattc
    25381 tcagtttgcg cgcggcagga tcgtacctca gatcgaggct gcccccctcg aagtgcaagg
    25441 gcaggtcccc ggcccagggc aggtacatgt ctccccgcga aaggtccaga gagaacgtgc
    25501 cgccgagcac cttgccctgt gacgtgagtt gcagattcac atctccgctc gcgggcatgc
    25561 tcaggaaatg cagcggcgag agggcgggaa atgtatccgc cagcgcgctg ggtatcaggt
    25621 cgcggacgga ggccttcagg ttcacctgtt ccgtttcccc cgaagcctcg gacgagaact
    25681 ggagcgtcca cgggcggccc ccggagtcga cggtgacgct gccctcgatg agactgcgca
    25741 gctttttgtg cgagagcgcg acgtcggcct gaggcacgcg ccagaccttc tgctccccgg
    25801 cgtgatcgac gatcagcgtc gcgtcgcgca gcccgatctg acgcaggaag gacgtcgcat
    25861 ccatgcgctg ccttgcccgg gcagaggcct ccgcgatcac ccgggcgagg ccgagctgcc
    25921 tcaacgccac cggaagcccg gatcccgcat cgcggccctc gccggagctc tgctcggtag
    25981 ggagcgtctg cacgtcgctg ccgccgggca cgccttcgcc ggcggacccg gctggacgcg
    26041 tgaagctgaa ggaaagcccc ccgtcctgcg aataggtgag gagcaggcgc ggttcgat
//
LOCUS       DASVDU010000052        15761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1283319, whole genome shotgun sequence.
ACCESSION   DASVDU010000052 DASVDU010000000
VERSION     DASVDU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15761
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283319"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1642
                     /gene="groL"
                     /locus_tag="VF226_03610"
     CDS             2..1642
                     /gene="groL"
                     /locus_tag="VF226_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109730.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7073104.1"
                     /translation="MAAKDVKFAADARERMMRGVDILANAVKVTLGPKGRNVILEKSF
                     GAPRTTKDGVTVAKEIELEDKFENMGAQMVREVASKTNDKAGDGTTTATVLAQAIVRE
                     GLKSVAAGMNPMDLKRGIDKAVAQAVEEIEKCAKKVKSSDEVAQVGTISANGETEIGK
                     MIAEAMQKVGNEGVITVEEAKSLETELEVVEGMQFDRGYLSPYFITNADKMVAELEDP
                     YILIHEKKLTGLQAMLPVLEAVVQTGKPLLIISEDVEGEALATLVVNKLRGGLKVCAV
                     KAPGFGDRRKAMLEDIAVLTGGTVISEDLGIKLENVTLDMLGRAKRVSVDKDNTTIVD
                     GAGKKKDIEARVAQIKVQIEETTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKER
                     KDRVDDALNATRAAVEAGVVPGGGVALLRAANAINVKGDNDDQDAGIQIVKRALQAPI
                     RQIADNAGVEGSIVVGKVLDSRSYNFGYDAQNDEYGDLIEKGIIDPAKVVKTALQDAA
                     SVASLLITTEAGIAEAPKKKEPAMPGAGMGGMGGMDMM"
     gene            complement(1830..2123)
                     /locus_tag="VF226_03615"
     CDS             complement(1830..2123)
                     /locus_tag="VF226_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011082959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX7073105.1"
                     /translation="MAHFVILSKFTHQGITDVKRTVERAEAGIQMAKKVNVTIKEIYW
                     TLGSRDVVVICEAPDDEAVTAFSLSLTSLGNVSNETLRAFSSDEMKQVLAKMV"
     gene            complement(2321..3118)
                     /locus_tag="VF226_03620"
     CDS             complement(2321..3118)
                     /locus_tag="VF226_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7073106.1"
                     /translation="MKYRLTILGCGSSGGVPRIGNDWGRCDPQNPKNRRRRCAALVEK
                     FGPEGVTTVLIDTGPDVREQLLSVGLKVLDGVLYTHDHADHTHGIDDLRPVAYAMKRR
                     VPVWFDARTRDSLVTRFAYCFETPPGGNYPPILEAHDINPPEPVRISGPGGVIEALPV
                     LQEHGDITSLGFRIGNFAYSPDISGLPETSIPLLKGLDLWVVDALRPTPHPSHFSLGQ
                     ALQWIERLQPKRAVLTHLTAELDYAAMKRELPDHIEPAYDGMVIELN"
     gene            complement(3115..3915)
                     /locus_tag="VF226_03625"
     CDS             complement(3115..3915)
                     /locus_tag="VF226_03625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7073107.1"
                     /translation="MLVDHHCHLDFPQFAAERDEVVARAARAGVGVIVTISTRIRQLP
                     DLLAIAEAYPNVFCSVGTHPHNAHEELDISAEEIVRLSEHPKVVAIGEAGLDYYYKKS
                     PPEAQAEGFRRHIAAARITGLPLEIHSRDADEDMARILEEEHAKGPFPAILHCFTGGR
                     DLAMRAVKLGLMVSFSGVITFRKSDELRAIAADVPLDQVLVETDAPFLAPEPYRGKTN
                     EPALVVHTAEVLARSKGVTPQEIATATTDNFFRWFRKVPHSAAAANGA"
     gene            complement(3915..5480)
                     /gene="metG"
                     /locus_tag="VF226_03630"
     CDS             complement(3915..5480)
                     /gene="metG"
                     /locus_tag="VF226_03630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7073108.1"
                     /translation="MAAPKNFYVTTAISYPNGVPHIGHAYEAIATDAIARFERLDGKD
                     VFFLTGTDEHGLKMKQTAAREGLTPRELADRNTPRFQAMVKALDISNDDFIRTTEERH
                     YRASEEIWRRMEKAGDIYLAKYAGWYSVRDEAYYAESETTVGDDGVRRGPQGTPVEWT
                     EEESYFFRLSAYQDRLLAHYEAHPDFILPPERRNEVVSFVRGGLEDLSISRTSLDWGI
                     PVPGNPKHVMYVWVDALTNYITGVGFPNENDPLWRYWPADLHVIGKDILRFHAVYWPA
                     FLMSAGLPVQHRVFAHGFLFNRGEKMSKSVGNVIDPFDLVKAYGVDPLRYFFLREVVF
                     GQDGSYSHEAIVNRINADLANDLGNLAQRSLSMISKNCGGTIPEPGAFSEADKAILDA
                     TDALYAQARAAMDRQGIKQYLDAVWGVVGETNRYFASEEPWAKRKTDFKRMETILYVT
                     AEVVRQVAILAQAVMPSSSAKLLDLLGQDADARSFAALGPGGRLKPGTAIPQPTGVFP
                     RYVEPEEASEGKA"
     gene            complement(5534..6673)
                     /locus_tag="VF226_03635"
     CDS             complement(5534..6673)
                     /locus_tag="VF226_03635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEX7073109.1"
                     /translation="MARAPAVAEIEVAPEADRLDGFPHPRETRHVFGHAAVERELAEI
                     LASRRIHHAWLLAGPEGIGKATLAYRFAAYALADAAARDVSLASLELPEGTPALRQVR
                     ALSHPGLLVIRRPWLQKEKRFAANIPVEEVRKLRNFLAHTAGGEARPDEAVQRVVIVD
                     QADELNASSANALLKSLEEPPPRTVFLLITSQAGRLLPTIRSRCRVLALSPLDPADLR
                     AAAEQALDAAELAKPAEADWPRLLHLARGSVRRLLALATSDGLKLARHVTAIFETLPK
                     IDWAAAHGLADDLSGSANEQRFETFFDLLLDTSARLTRAAATGEGEPDEVALARRLIP
                     DGRLASWAELWETVVREKADALALNLDRKTLVLQTLSRLEGASRG"
     gene            complement(6673..7335)
                     /gene="tmk"
                     /locus_tag="VF226_03640"
     CDS             complement(6673..7335)
                     /gene="tmk"
                     /locus_tag="VF226_03640"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7073110.1"
                     /translation="MARGKFITFEGGEGAGKSTQARLLADRLQARGIHVVLTREPGGS
                     AFAEQVRQLILDPGTADHSALSEALLFSAARADHLERTIRPALEAGHWVICDRFSDST
                     RVYQGVAGGLPKGVIEALERIVVDDTVPDVTIVLDLPASDGLERARGRSGKAMPAGPD
                     ADNYEGRPLSYHERLRDGFLMIATAEPERCVVVDATQKIEEVAEQVWTSIEMRLIQQQ
                     GD"
     gene            complement(7379..8581)
                     /locus_tag="VF226_03645"
     CDS             complement(7379..8581)
                     /locus_tag="VF226_03645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX7073111.1"
                     /translation="MTERIPHMRSQLVARWLQLVLAVVAACTFAIGPAWAQAGYSTEA
                     KQAILIDADTNAILFEHNADQPMHPASMSKLMTLVMAFKAIRSGQLKLEDEIQMSVNA
                     WRSGGAPSGTSAMFVPVGEKVTVNELLQGIIVQSGNDASIALAEALAGTEQAFAQLMT
                     LHAREIGLKSSTFKNSTGLHDPQHLTTARDLAKLAQHVIKEYPEFYPMFAQPEFRYRR
                     HRFINRNPLLKMDIGADGLKTGYTEESGYGIVASAKQDGRRLIVVVNGLPTAAKRRDE
                     AARLLQWGFRSFSEFRLFDEGEIVGRARVWGGDQFYLPLTGDDGGVSVWLPRAVANQR
                     LKAEIVYNSPLKPPIKKGEPVAKLRVTSAMNTTSEVPLYAAEDVAAAGIMRRGLDTLA
                     YMAFRWIP"
     gene            8950..10035
                     /locus_tag="VF226_03650"
     CDS             8950..10035
                     /locus_tag="VF226_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073112.1"
                     /translation="MGPTDSFVRFEKVDKTYDGSVLVVCGLDLEIRRGEFLTLLGPSG
                     SGKTTTLMMLAGFETPTRGVITLEGADITRVPPHKRGLGMVFQNYALFPHMTVAENLA
                     FPLQVRGMSKTAVREKVARALEMVRLPDLGDRLPSQLSGGQQQRIALARALVFEPLVV
                     LMDEPLGALDKQLREHMQYEIKQLHAQLGVTVVYVTHDQSEALTMSDRIAVFHEGRIQ
                     QVGDPRTIYDHPDTAFVAQFIGENNRIEGTVRQVSGGVAAVAISNGTTVLAEAGPGIA
                     AGDRALVSLRPERLRLGNANSDSANRFAARILEVIYHGDHLRVRLEALGRDDFIVKTP
                     VLGAEGLTIGTEVPISWREVDGRALPL"
     gene            10151..11206
                     /locus_tag="VF226_03655"
     CDS             10151..11206
                     /locus_tag="VF226_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018495505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073113.1"
                     /translation="MRVGKKAILATAVAATIGLTAYGALANRPLTVVSWGGAYQDAQK
                     KVYFEPFKATGVQLIDESWDGGVGVLRSKVQGGTDSGWDVVQVESEELELGCDEGMFL
                     PIDWDRIGGKDRYLPAAQHECGVGTIMWNFVLAYDKDKLKEAPRGWADFFDTKKFPGK
                     RALRQGPKSNLEIALMADGVAPADVYKVLATDEGVDRAFKKLDEIKNDIVWWKSGAQA
                     PQLLASGEVVMTSAYNGRIDAAVRNDKRNFGIQWNGTLYTLDSWVILKTSPNVDEAYK
                     FLEFVGRAENQAKLPVHIAYGVTNVKANEQIDPKRLVDLPTHPDNIKNGVQISDRFWI
                     ENIDRLTERFNTWAAQN"
     gene            11261..12493
                     /locus_tag="VF226_03660"
     CDS             11261..12493
                     /locus_tag="VF226_03660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073114.1"
                     /translation="MAGQKELLRDLKRAERRRRLMALALIAPLFLFLLISFVLPIGDM
                     LRRSLVDSELSEVWPRTAALTKVWVWDRAQDPTPEMYRALGQDIKESARERTIAVAAR
                     RLNYALENGRSLVMNTGRELRRDAADPPDWRARLIEIDPAWGERKTWIAIGQAAGPLT
                     DFYLLTALDLERNEKNKIVSRPPERSLYVNVLGRTLLISVSVTLLTLVLGFPVAYLVA
                     NAPKRIAGLLMILVLLPFWTSLLVRSAAWIVLLQDQGLVNDALQAVGLTSAPIRLIFN
                     RTGVIVAMTHVLLPFMILPMMATMKAIPPTYMRAALSLGAHPAIAFFRVYLPQTLPGV
                     AAGVLLVFIMALGYYVTPALVGGAEDQMLAYFIAFYTTSSANWGLAAALGVMLLTATA
                     ILYLVYARLVGMGQVKLG"
     gene            12499..13326
                     /locus_tag="VF226_03665"
     CDS             12499..13326
                     /locus_tag="VF226_03665"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073115.1"
                     /translation="MRGARTRTEQVAKVVLVVSAALVCFFLIAPILAIMPLSVNDSEF
                     LTYPLRGFTTRWYEAVFSSDKWRIAVVNSFLIGLPAAFIATVLGTLAAIGLSMADFRG
                     KAAVMTLLLSPLVVPIVIVAVGLYFFFAPFGLTQSYTGLIIAHAALGAPFPVVTVSAA
                     LASFDRNLARAAASLGAPPLTAFRRVMLPLVAPGVAAGALFAFAISFDEVVTVLLLGA
                     PHQRTIPREMFSGLRDSFNPTIAAVAVMLTLLAVVLLLIVELLRARSERMKQQASSM"
     gene            complement(13464..>15761)
                     /locus_tag="VF226_03670"
     CDS             complement(13464..>15761)
                     /locus_tag="VF226_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017274732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7073116.1"
                     /translation="IGVQLSPTGNALATSKAIHAKMEELSRFFPPGLEYSIPYDTSPF
                     VEISIEKVLHTLLEAVGLVFLVMLLFLQSFRYTIIPTIVVPVALLGTCAVMLATGFSI
                     NVLTMFAMVLAIGILVDDAIVVVENVERIMSEEGLPPREATRKAMGQITGAIIGITLV
                     LTAVFVPMAFFPGAVGIIYQQFSLTMVVSILFSGFLALSLTPALCASFLKPIPAGHHL
                     EKRGFFGWFNRGFSKTTRGYSGLVGYVARRAGRFMVIYLALVAGLGWAYLRLPTAFIP
                     NEDQGYLIVDMQAPPEASANRTIDVIKQVEDALLADEAVDRVFTIQGFSFSGAGQNAG
                     LAFVTLKDWSVRGPENSAQAIAARANAKLWQIRDGITFALSPPPIQGLGTTSGFTFRL
                     QDRGGLGQAALAAARDQLLAAAAKSPVLTGLRVEGLPDAAQVNVIIDREKANTFGVAF
                     SDINATISANLGSSYVNDFPNAGRMQRVVVQAGEQARMQTEDLLNLNVRNAAGGMVPL
                     SSFAHVEWVKGPTQVVGYNGYPSVRISGQAAPGFSSGDAIAEMERLAAELPQGFGYEW
                     TGQSLQEIQSGALAPLLIALSIVFVFLLLAALYESWSIPLSVMLVVPLGMIGSVIAVT
                     LRGMPNDVYFMVGLIAIIGLSSKNAILIIEFAKDLRAEGRSVIEATIEAARLRFRPIL
                     MTSLAFTLGVLPLAIATGASAVSQNAIGTGVLGGIISATILGLIFVPAFFVFVMRIFG
                     RSRKAGPDAPKPAETKQLEVATGEA"
BASE COUNT         2845 a         5122 c         5095 g         2699 t
ORIGIN      
        1 aatggcagcc aaagacgtca agttcgctgc tgacgcgcgc gagcgcatga tgcgcggcgt
       61 cgacatcctc gccaacgcgg ttaaggtcac gctcggcccc aagggccgca acgtcattct
      121 cgagaagtcg ttcggcgcac cgcgcaccac gaaggacggc gtgacggtcg cgaaggagat
      181 cgagctcgag gacaagttcg agaacatggg cgcgcagatg gtgcgcgagg tcgcctcgaa
      241 gaccaacgac aaggcgggcg acggcaccac cacggccacc gtcctcgccc aggcgatcgt
      301 gcgcgagggt ctgaagtcgg ttgccgccgg catgaacccg atggacctca agcggggcat
      361 cgacaaggcg gtcgcccagg ccgtcgagga gatcgagaag tgcgccaaga aggtcaagag
      421 ctccgacgag gtcgcccagg tcggcaccat ctccgccaac ggagagaccg agatcggcaa
      481 gatgatcgcc gaggccatgc agaaggtcgg caacgagggc gtgatcacgg tcgaggaggc
      541 caagagcctg gagaccgagc tcgaggtcgt cgagggcatg cagttcgacc gcggctatct
      601 ctcgccgtat ttcatcacca acgccgacaa gatggtcgcc gagctcgagg atccctacat
      661 cctgatccac gagaagaagc tgaccggcct gcaggccatg ctgccggtgc tcgaggccgt
      721 cgtgcagacc ggaaagccgc tgctcatcat ctccgaggac gtcgagggtg aggccctggc
      781 gacgctcgtg gtcaacaagc tgcgcggcgg cctcaaggtc tgcgcggtga aggctccggg
      841 cttcggcgac cgccgcaagg ccatgctcga ggatatcgct gttctgaccg gcggcaccgt
      901 catctccgag gatctcggca tcaagctcga gaacgtcacc ctcgacatgc tgggccgcgc
      961 caagcgcgtt tccgtcgaca aggacaacac gaccatcgtc gacggcgccg gcaagaagaa
     1021 ggacatcgag gcccgcgtcg cccagatcaa ggtgcagatc gaggagacca cctccgacta
     1081 tgaccgcgag aagctgcagg agcgtttggc gaagctcgcc ggcggcgttg cggtgatcaa
     1141 ggtcggcggc gccaccgagg tcgaggtgaa ggagcgcaag gaccgcgtcg acgacgctct
     1201 caacgcgacc cgcgcggccg tcgaggccgg cgtggttccg ggcggtggcg tcgctctgct
     1261 ccgggccgcc aacgcgatca acgtcaaggg cgacaacgac gatcaggatg ccggaatcca
     1321 gatcgtcaag cgtgcgctgc aggcgccgat ccgccagatc gccgacaatg caggcgtcga
     1381 gggctcgatc gtcgtcggca aggtgcttga ctcgcggtcg tacaacttcg gctacgacgc
     1441 tcagaacgac gagtacggcg acctgatcga gaagggcatc atcgacccgg ccaaggtcgt
     1501 gaagaccgcc ctgcaggacg ccgcctcggt tgccagcctg ctcatcacga cggaggccgg
     1561 catcgccgag gctccgaaga agaaggagcc cgcgatgccg ggcgccggca tgggcggcat
     1621 gggcggcatg gacatgatgt aagcccgttt ggcgatcggc cgaggtgatg ccaccgaagc
     1681 cgatcactgc acggcaagaa aaggtctccc cagacccaga gagggccgcc cgggcagccg
     1741 gggcggccct ttcgattcta atgacgaggc atcctggctc ggtttcgggc ctgggccgca
     1801 agcgccacgc cgcgcacgct ggtcgaggct cagaccatct tggcaagaac ctgtttcatc
     1861 tcgtccgagg agaacgcgcg cagtgtctcg ttgctgacat tgccgagcga cgtcaggctc
     1921 aaggagaacg ccgttaccgc ctcatcatcg ggcgcctcgc agatgacgac gacgtcgcga
     1981 gagcccagcg tccaatagat ctccttgatc gtcacgttga cctttttggc catctggatg
     2041 ccggcctcgg cacgctcgac ggtgcgcttg acgtcagtga taccttggtg cgtgaacttg
     2101 gaaagtatga cgaagtgggc catgggtgtc tctccttcga ccacccccca cacagaatga
     2161 atagcacata caccaggaaa agcgtgggat cgtccgcctg ttagggccaa tggtgaatcc
     2221 gactgcctcc gtcgaaacct gggttacggc tcgccgttca catttggcct aaagcggcat
     2281 cggccccaaa tgcggaatcc ctagtcagcc gccatatcgg tcaattcagc tcaatcacca
     2341 tgccgtcgta ggctggctcg atgtgatcgg gcaattcccg tttcatcgcg gcgtagtcga
     2401 gctcggccgt caaatgggtg aggacggcgc gcttgggctg caaccgctcg atccactgga
     2461 gcgcctggcc caggctgaaa tggctcggat gcggagtggg gcggagcgca tcgaccaccc
     2521 agagatcgag acccttcaga agcggtatcg aagtttccgg cagcccgctg atgtcgggcg
     2581 agtacgcgaa gttgccgatg cggaaaccga gcgaggtgat atcgccgtgc tcctgcagca
     2641 ccggtagcgc ttcgatcacg ccgccaggcc cggaaatgcg caccggctcc ggcggattga
     2701 tgtcgtgggc ttcgagaatt ggcggataat tcccgccggg cggtgtctcg aagcagtagg
     2761 cgaagcgggt gacgaggctg tcccgcgtcc gcgcatcgaa ccacaccggc acgcgacgct
     2821 tcatggcata ggcaacgggc ctcagatcgt cgatgccgtg ggtatggtcg gcgtggtcgt
     2881 gcgtataaag cacaccgtcg agcaccttca gcccgaccga gagcagctgc tcgcggacat
     2941 cgggcccggt gtcgatgagc acggttgtca cgccctcggg tccgaacttc tcgacgagcg
     3001 ccgcgcagcg ccggcggcgg ttcttcgggt tctgcggatc gcagcgcccc cagtcgttac
     3061 cgatgcgcgg cacgccgccc gaggagccgc agccaaggat cgtcagccgg tacttcatgc
     3121 tccgttcgcc gccgctgccg aatgcggaac cttcctgaac catcggaaga aattatccgt
     3181 cgtcgccgta gcgatctcct gcggggtcac gcccttggaa cgtgccagca cctccgccgt
     3241 atgcacgaca agggccggct cattggtctt gccgcggtac ggttcgggcg cgaggaacgg
     3301 cgcgtcggtc tccaccagca cctggtcgag gggcacgtcg gcagcaatgg cccgcagctc
     3361 atcggatttg cggaaggtga tgacgcctga gaaggagacc atcagcccca gcttcaccgc
     3421 cctcatcgcc agatcgcgcc cgccggtgaa gcaatgcagg atcgccggaa agggcccctt
     3481 cgcgtgctcc tcttccagaa tgcgtgccat gtcctcgtcg gcatcacggg aatggatttc
     3541 aagcggaagg ccggtgatgc gtgccgccgc gatgtgacgg cggaagcctt cggcctgcgc
     3601 ctcgggcggg ctcttcttgt aatagtagtc gagccctgcc tcgccgattg ccacgacttt
     3661 cggatgctcc gacaaccgca cgatctcctc ggcggagata tccagctcct cgtgcgcatt
     3721 gtgcggatgg gtgccgaccg agcagaacac gttcggatag gcttcggcga tggcgagcag
     3781 atccggaagc tggcgaatgc gcgtcgatat ggtgacgatc acgccgacgc ccgcccgcgc
     3841 ggcgcgggcg acgacctcgt cgcgctcggc cgcgaactgc ggaaagtcca ggtggcagtg
     3901 atggtcgacc agcatcaggc cttgccctcg ctcgcctcct cgggctcgac gtaccgagga
     3961 aacacgccgg tcggctgcgg gatcgccgtg ccgggcttca gccggccacc ggggcccaga
     4021 gcggcgaagc tgcgcgcgtc cgcatcctgc ccgagcaggt cgagcaactt ggcggacgac
     4081 gacggcatca ccgcctgggc gaggatcgcc acctgccgga cgacctcggc cgtcacatag
     4141 aggatcgtct ccatgcgctt gaagtcggtc ttgcgcttgg cccagggctc ctcgctggcg
     4201 aagtagcggt tcgtctcgcc gacaacgccc cagacggcgt cgaggtattg cttgatcccc
     4261 tgccggtcca tggccgcccg cgcctgcgca taaagcgcat ccgtggcgtc gagaatggcc
     4321 ttgtccgcct cgctgaaggc gcccggctcg ggaatggtgc cgccgcagtt cttcgagatc
     4381 atggacagcg agcgctgtgc caggttgccg agatcgttcg ccagatccgc gttgatacgg
     4441 ttgacgatcg cctcgtggct gtagctgccg tcctgcccga acacgacttc gcgcaggaag
     4501 aaatagcgca gcgggtcgac gccgtaggcc ttcacgagat cgaacgggtc gatgacgttg
     4561 cccaccgact tcgacatctt ctcgccgcgg ttgaacagga acccatgcgc gaagacgcga
     4621 tgctggacgg ggaggccggc cgacatcagg aacgccggcc agtacaccgc gtggaagcgc
     4681 aggatgtcct tgccgatgac atgcagatca gccggccaat agcgccagag cggatcgttc
     4741 tcgttcggaa agccgacccc ggtgatgtag ttggtcagcg catcgaccca cacgtacatc
     4801 acgtgcttcg gattgccggg aacagggatg ccccagtcga gcgacgtgcg cgagatcgac
     4861 aagtcctcga gcccgcccct gacgaagctc acgacctcgt tgcgccgttc gggcggcagg
     4921 atgaagtcag ggtgggcctc atagtgcgcg agcaaccggt cctggtaggc ggaaaggcgg
     4981 aagaaatagg actcctcctc cgtccactcg accggcgttc cctgcgggcc gcggcgcacg
     5041 ccatcatcgc cgacggtcgt ctcgctctcg gcatagtagg cttcgtcacg caccgagtac
     5101 cagccggcgt acttggcgag atagatgtcg cccgccttct ccatgcgccg ccagatctcc
     5161 tcggaagcgc gatagtggcg ctcctcggtg gtacggatga aatcgtcgtt cgaaatgtcc
     5221 agtgccttca ccatggcctg gaagcgcggc gtgttccggt cggcgagctc gcgcggggtg
     5281 agtccctccc tggctgccgt ctgcttcatc ttgaggccgt gctcgtccgt gcccgtcagg
     5341 aagaacacgt ccttgccgtc gagccgctcg aaacgggcga tggcatcggt cgcaatggcc
     5401 tcgtaggcat gcccgatgtg gggcacgcca ttcggatagg agatggccgt ggtgacgtaa
     5461 aagttcttag gcgcagccat aggtttcgct tgggttccag tcgagcgaca cccccggaag
     5521 aacgacaacg agcttacccg cgggaggctc cctccaggcg ggagagggtc tgcagcacga
     5581 gcgttttgcg gtcgaggttg agcgcgaggg cgtcggcctt ttcgcgcacg actgtttccc
     5641 acagctctgc ccacgaggca agcctgccgt ccgggatcag ccgccgtgca agggccactt
     5701 catccggctc tccttcgccc gtcgcggccg cccgggtgag gcgggcgctc gtgtcgagga
     5761 ggaggtcgaa aaaggtctcg aagcgctgct cgttggccga gccggaaaga tcatccgcaa
     5821 ggccgtgggc tgcggcccag tcgattttcg gcagggtctc gaaaatagcg gtcacatgcc
     5881 tggccagctt caggccgtcg ctggtcgcga gcgccagcag ccggcgcacg ctgccgcggg
     5941 cgagatgcag aagccgcggc cagtccgctt cggcgggctt cgccagctcc gcggcatcga
     6001 gcgcctgttc ggcggcggcg cgcagatcgg ccgggtcgag cgggctcaac gccagcacgc
     6061 ggcagcggga gcggatcgtc ggcagcagcc gaccggcctg tgaggtgatc agcaggaaca
     6121 ccgttcgggg cggcggctcc tcgagcgatt tcaggagagc gttggcggag gatgcattga
     6181 gctcgtcggc ctggtcgacg atgacgacgc gctgaacggc ctcatccggc cgtgcctccc
     6241 caccagcggt atgggccaga aagttcctga gcttacgcac ctcttcgacg gggatgttgg
     6301 ctgcgaagcg cttctccttt tgcaaccaag gccgccggat caccagcagg ccagggtgcg
     6361 acagcgcccg aacctgccgc agcgccggag tgccctccgg cagctcgagg ctcgccagcg
     6421 agacatcacg cgcagcggca tcagccagcg cataagccgc gaagcggtag gcgagcgtcg
     6481 ccttgccaat cccctctggc ccggccagaa gccaggcatg atggatacgg cgcgaggcaa
     6541 ggatttcggc aagctctcgc tcgacggcgg catggccgaa cacgtggcgc gtttctctgg
     6601 ggtgcggaaa gccgtcgagc cgatcggcct cgggcgccac ctcgatttcc gcgacagcgg
     6661 gcgcccgcgc catcaatcac cctgctgctg gatcaaccgc atctcgatgc tggtccatac
     6721 ctgctcggcc acctcttcga tcttctgcgt cgcatcgacg accacgcatc gttcgggctc
     6781 ggccgtggcg atcatcagga aaccgtcacg cagccgctcg tggtaggaca aggggcgacc
     6841 ctcgtagttg tcggcatccg ggccggccgg catagccttg ccgctccggc ctcgggctcg
     6901 ctcgagcccg tcagaggccg gcaaatcgag gacgatcgtc acgtcgggaa cggtatcatc
     6961 gacgacgatg cgctcgagag cctcgatgac gcccttcggc agaccaccgg ccacgccctg
     7021 atagacccgc gtcgaatcgg aaaagcggtc gcagatcacc caatgtcccg cctccagcgc
     7081 cggccggatc gtgcgctcca gatggtcggc gcgggcggcg ctgaacagaa gcgcctcgga
     7141 gagcgcactg tgatccgccg tgccggggtc gagaatgagc tggcgcacct gttcggcgaa
     7201 ggcagaaccg cccggctcac gcgtcaggac gacatggatg ccgcgcgcct gcagacggtc
     7261 ggccaggagc cgcgcctggg tcgacttccc cgccccctcg ccgccctcga acgtgatgaa
     7321 cttgcctctc gccatggcac cgctcgtttc tgtcgcgcgg gcgcgccgac tggtggactc
     7381 aagggatcca gcggaaggcc atataagcaa gcgtgtcaag gccgcggcgc atgatccctg
     7441 ccgcggcgac atcctcggca gcgtagagcg gcacctcgct ggtcgtgttc atggcgctgg
     7501 taacacgcag cttggcgaca ggttcgcctt tcttgatcgg tggcttcagc gggctgttgt
     7561 agacgatctc cgccttgagc cgctgattgg ccacggcacg cggcagccag acgctcaccc
     7621 cgccatcgtc cccggtcagc ggcagataaa attgatctcc gccccacacg cgcgcccgac
     7681 cgacgatctc gccctcgtcg aacagccgga actcgctgaa gctccggaag ccccactgca
     7741 gcaggcgcgc ggcctcgtca cggcgcttcg cggcggtcgg caggccgttg acgacgacga
     7801 tcaggcgccg gccgtcctgt ttggccgagg cgacgattcc gtatccgctt tcctcagtgt
     7861 accctgtctt caacccgtcg gcgccgatat ccatcttcag cagcgggttg cgattgatga
     7921 accggtgcct gcgataccgg aattccggct gcgcaaacat cggatagaac tcggggtatt
     7981 ccttgatgac gtgctgcgcg agcttggcga gatcgcgcgc cgtggtgaga tgttggggat
     8041 cgtgcaaccc cgtcgagttc ttgaaggtcg acgatttgag gccgatctcg cgcgcgtgca
     8101 gcgtcatgag ttgagcgaag gcctgctcgg taccagccaa ggcctcggca agcgcgatcg
     8161 aagcgtcgtt gcccgactgc acgatgatgc cctgcagcag ctcgttcacc gtcaccttct
     8221 cgccgacggg cacgaacatg gccgaagtgc ctgatggcgc gccgccgctg cgccaggcgt
     8281 tcacgctcat ctggatctcg tcttccagct tgagctgccc tgacctgatg gccttgaacg
     8341 ccatcaccag ggtcatcagc ttgctcatgc tggccgggtg catgggttga tcggcgttgt
     8401 gctcgaagag aatggcgttg gtatcggcgt cgatcagaat cgcctgcttg gcttccgtgg
     8461 aatagccagc ctgagcccac gcaggtccaa tggcgaatgt gcacgctgca acgaccgcca
     8521 gcacgagctg gagccagcgg gcaaccaatt gagatcgcat gtgcggaata cgttcagtca
     8581 tagctctcca gtctgttcgc ctgaggcctg actcgtagaa ctatcacgcc accagcccaa
     8641 gtgcgtcaat gccggcggac gagggtgaga cgccgcttgg tgaacgaaat cacaatgaca
     8701 ccgggccagc gcaggcggac gccttctatc cgtggcgtgt tctcaagtcc aatccgcccc
     8761 ccttatcaac acacgcaagc cactctggaa tcgttgcggc ccgctgggcc ggctgctaaa
     8821 cgactgcgca acgggtgccc agggcttgcg tggctggatt tcgggtcgcg gccggacgcc
     8881 agcccgacag aggtccgtaa cccgaaagcg ttcgttcact ccagcatcgg acggttcgac
     8941 tgaggtttga tgggtccaac tgatagcttc gttcggtttg aaaaggtcga caagacctac
     9001 gacggttcag tgctggtggt atgtggcctg gatctcgaga tcaggcgtgg cgagttcctg
     9061 acgctcctcg gtccatcggg ctcagggaaa acgacgacct tgatgatgct ggccggcttc
     9121 gagacgccga cgcgcggcgt catcacgctc gaaggtgccg atatcacgcg agtccctccg
     9181 cacaagcgtg gcctgggcat ggtgttccag aactacgccc tctttccaca tatgacggtt
     9241 gctgaaaacc ttgctttccc gctgcaggtc cgcgggatgt ccaagacggc cgtccgcgag
     9301 aaagttgcac gcgcactcga aatggtccgc ctgccggatc tcggggacag gctgccctcg
     9361 caactgtctg gcggacagca gcagcggatt gcactcgccc gggcgctcgt attcgagccg
     9421 ctggtcgtcc tcatggacga gccgcttggc gctctcgaca agcagctccg ggagcacatg
     9481 cagtacgaga tcaagcaact gcacgcgcag ctcggcgtca cggtggtcta tgtcacccac
     9541 gatcagagcg aggcgctgac catgtccgac cgcatcgccg ttttccacga gggccggatc
     9601 cagcaggtcg gcgatcccag gaccatttac gatcatccgg acacggcgtt cgtcgcgcag
     9661 ttcatcggcg agaacaaccg gatcgaaggc acggtccgcc aggtctccgg cggcgtcgcc
     9721 gccgtcgcga tcagcaacgg caccacggtg cttgcggaag ccggtcctgg catcgccgcc
     9781 ggcgaccgcg ccctggtttc actacgaccc gagcgtctcc ggcttggaaa tgccaacagc
     9841 gactcggcca acaggttcgc agcccggatc ctcgaggtga tctatcacgg cgatcacctt
     9901 cgcgtgcggc tcgaggcact ggggcgggat gatttcatcg tcaagacacc cgtgctcggt
     9961 gccgaggggc tgaccattgg cacagaggtg ccgatctcgt ggcgggaggt cgacgggcgc
    10021 gccttgccgc tctgaaaacg caggaatctt gccgcttcaa aagctcagga atactgtcac
    10081 caccgcgatc tgtggccgcg aaatccggca gacagaagag actgggaaac ccctgtttgg
    10141 gagggtaacg atgagagtag gaaagaaagc gatactcgcg accgccgtcg cggccaccat
    10201 tggcctgact gcatatggcg cgctggcgaa ccggccgctg acggtcgttt cctggggcgg
    10261 cgcctatcag gacgcgcaga agaaggtcta cttcgagccc ttcaaggcca ccggcgtcca
    10321 gttgatcgac gagagctggg atggcggcgt cggcgtgctg cgctcgaagg tccagggcgg
    10381 cacggattcc ggctgggacg tcgttcaggt cgaaagcgag gagctggagc tcggctgcga
    10441 cgagggcatg tttctgccga tcgattggga cagaatcggc ggcaaggacc gctatctgcc
    10501 ggccgcccag catgaatgcg gcgtcggcac catcatgtgg aacttcgtcc tcgcctacga
    10561 caaggacaag ctgaaggagg cgccccgcgg ctgggccgac ttcttcgaca cgaagaagtt
    10621 cccgggcaag cgggccttgc gtcagggccc gaagagcaat ctggagatcg cactgatggc
    10681 cgacggcgtg gcgccggccg acgtctacaa ggtgttggcg acagatgaag gtgtcgaccg
    10741 cgccttcaag aagctcgacg agatcaagaa cgacatcgtc tggtggaagt cgggcgcgca
    10801 agcgccgcag ctcctcgcgt ctggcgaggt cgtcatgacg tcggcctaca acggccggat
    10861 cgacgcggcc gttcgcaacg acaagcggaa cttcggcatc cagtggaacg gcacgcttta
    10921 cacgctcgac agctgggtaa tcctcaagac ctcgccgaat gtggacgagg cctacaagtt
    10981 cctcgagttc gtcggccgtg ccgaaaacca ggcgaagctg cccgtccaca tcgcttacgg
    11041 cgtgacgaac gtgaaggcta acgagcagat cgatccgaag cgcctcgtgg acctgccaac
    11101 acacccggac aacatcaaga acggtgtaca gatctcggat cgattctgga tcgagaacat
    11161 cgatcggctg accgagcgct tcaacacctg ggccgcgcag aactgacgtg atgcaggaaa
    11221 ggccggaagc acgtttgctt ccggccgttt tgggttccca atggctgggc aaaaggaact
    11281 gctgcgcgac ctgaagcgcg ccgaacggcg ccgtcgactg atggcgctcg cgctcattgc
    11341 cccgctgttc ctctttctgc tgatctcgtt cgtgctgccc atcggcgaca tgctgcgccg
    11401 gtcgctggtg gactccgagc tttccgaagt ctggcctcgg acggctgccc tcacgaaagt
    11461 ctgggtctgg gaccgggccc aagatccaac cccggaaatg taccgggcgc ttgggcaaga
    11521 tatcaaagag tcggcccgcg agcgcaccat cgccgtcgcc gcgcgccggc tgaattatgc
    11581 attggagaat ggccggagcc tggtcatgaa caccgggcgg gagctgcgcc gcgacgcggc
    11641 cgacccgcca gactggcgcg cccgtctcat cgagatcgat cccgcctggg gtgagcgcaa
    11701 gacctggatc gccatcgggc aggctgccgg gccgctcacg gatttctatc tcctgaccgc
    11761 tctcgatctc gagagaaacg aaaagaacaa aatcgtttcg cgcccccctg aaagatcgct
    11821 ttacgtcaat gtgctggggc gcacgctgct gatctcggtt tccgtcaccc tgctcacgct
    11881 ggtgcttggc tttcccgtgg cctacctcgt cgcgaatgcg cccaagcgca tcgccggatt
    11941 gctgatgatc ctggtgctgc tgccgttctg gacatccctg ctcgtccggt cggcggcgtg
    12001 gatcgtgctg ctgcaggatc aggggctcgt caacgacgcg ctgcaggcgg tcggcctgac
    12061 gagcgcaccc attcgtctca tcttcaatcg cacgggtgtg atcgtcgcga tgactcacgt
    12121 tctgttgccg ttcatgatcc tccccatgat ggcgacgatg aaggcgatcc cgccgaccta
    12181 tatgcgcgcc gccctgtcgc tcggcgcgca tccggccatc gcgttcttcc gggtttacct
    12241 gccgcagacc ctgcccggcg tcgcggccgg agtgctgctc gtcttcatca tggcgctcgg
    12301 ctactacgtg acgccggcgc tggtcggcgg ggctgaggac cagatgctcg cctacttcat
    12361 cgccttctac acgacctcga gcgccaactg gggtctcgcc gcggctctcg gcgtgatgct
    12421 gctgaccgcg acggccattc tctacctggt ctatgcgcgg ctcgtcggca tgggccaggt
    12481 gaagctgggg taggcacgat gcgcggcgcc aggacccgta ccgagcaggt ggccaaagtc
    12541 gtactcgtcg tgtcggcggc gctggtgtgc ttcttcctga tcgcgccgat cctggcgatc
    12601 atgccgcttt cggtaaacga ctcggagttc ctgacctatc cgctgcgcgg cttcaccacc
    12661 cgctggtacg aagcggtatt ctccagcgac aagtggcgca tcgcggtggt gaactcgttc
    12721 ctcatcggac tgccggcggc cttcatcgcg accgtgctgg gcacgctggc ggcgatcggt
    12781 ctgtccatgg cggacttccg gggcaaggcg gcggtcatga cactactgct ctcaccgctg
    12841 gtcgtgccca tcgtgatcgt cgccgtcggg ctctatttct tcttcgcgcc gttcggcctg
    12901 acgcaatcct acacagggct catcatcgcc cacgccgccc tcggcgcacc cttcccggtc
    12961 gtgacggtgt cagccgccct cgcctccttc gaccggaacc ttgcgcgggc tgccgcaagt
    13021 ctcggcgcac cgccgctcac ggccttccgc cgcgtgatgc tgccgctcgt cgctccagga
    13081 gtcgcggcgg gggcgctgtt cgcgttcgcg atctccttcg acgaggtcgt gacggtgctg
    13141 ctgctcggcg ctccgcacca gcgcacgatt ccacgcgaga tgttctccgg cctgcgcgac
    13201 agcttcaacc cgacgatcgc cgccgtcgcc gtgatgctga cccttctcgc cgttgtgctg
    13261 ctgctcatcg tcgagttgct gcgcgcgcgc agcgaacgca tgaagcagca ggccagcagc
    13321 atgtgaacct gaacccgctc ggtggctcac gtctgccaag cgggctcgtt cttctgctca
    13381 taatcgctga aggccttctc gagaccgccg atcacctcgg ccgacgtctc gaacatggca
    13441 caggtggctg aaacgcagac gcgtcaggcc tcgccggtgg cgacctcgag ctgcttcgtc
    13501 tcggccggct tcggcgcatc cggcccagct ttccgggagc ggccgaagat gcgcatgacg
    13561 aacacgaaga acgccggaac gaagatgagt cccaggatcg tggctgagat gatgccgccc
    13621 aagacacccg ttccaatggc gttctggctc accgcactgg ctcccgttgc gatcgccagc
    13681 ggtaagacgc ccagcgtaaa ggcgagcgag gtcatcagga tcggccggaa gcgcaggcgc
    13741 gccgcctcga tggtcgcctc gatcaccgat cgaccctcgg cgcgcaagtc ctttgcaaac
    13801 tcgatgatca ggatcgcgtt cttcgacgac aggccgatga tcgcgatcag gccgaccatg
    13861 aagtagacgt cgttcggcat gccgcgcagc gtcaccgcga tcaccgagcc gatcatgccc
    13921 agcggaacaa ccagcatcac cgacagcgga atggaccagc tctcgtagag cgcagccagc
    13981 agcaggaaca cgaacacgat gctgagcgca atgagcagcg gtgccagggc tcccgactgg
    14041 atctcctgca acgactggcc ggtccactcg tagccgaacc cttgcggaag ctcggctgcg
    14101 agccgctcca tttccgcgat ggcatcgccc gaggagaagc ccggtgcggc ctgcccgctg
    14161 atgcgcacgg acgggtagcc gttgtagccg acgacctgcg tcgggccctt cacccactcc
    14221 acgtgcgcga aggacgacag cggaaccatg ccgcccgcag cgttgcggac gttgaggttg
    14281 agcaagtcct ccgtctgcat gcgggcctgc tcgcccgcct gcacgaccac gcgctgcatg
    14341 cggccggcat tcgggaagtc gttgacgtag ctcgagccga ggttggccga gatggtggcg
    14401 ttgatgtccg agaacgcgac gccgaaggtg ttcgccttct cgcggtcgat gatcacgttg
    14461 acctgcgccg cgtccggcaa accttcgacc cgcagaccgg tgagcaccgg gctcttggca
    14521 gctgccgcga gcagctgatc tctggccgca gccagtgccg cctgacccag cccgcctcga
    14581 tcctgcaggc ggaacgtgaa gccgctcgtc gtcccgagcc cctgaatcgg cggaggtgac
    14641 agcgcaaacg tgatcccgtc cctgatctgc cagagcttgg cattggcccg cgctgcgatc
    14701 gcctgggccg agttctcggg cccgcggacg ctccagtcct tgagggtgac gaacgccagt
    14761 ccggcgttct gtccggcgcc cgaaaagctg aagccctgga ttgtgaacac ccgatcgacg
    14821 gcctcgtccg caagcaaggc atcctcgacc tgcttgatga cgtcgatggt gcggttggcg
    14881 ctggcttcgg gcggagcctg catgtcgacg atcagatagc cctggtcctc gttcgggatg
    14941 aacgccgtcg gcagccgcag ataggcccat ccgagcccgg cgaccagcgc caggtagatc
    15001 accatgaacc gcccggcgcg ccgcgcgacg tagccgacga gcccgctgta accgcgggtg
    15061 gtcttgctga agccgcggtt gaaccagccg aagaagcccc gcttctccaa gtgatgtccg
    15121 gccgggattg gcttcaggaa cgaggcgcag agcgctggcg tcagcgacag cgcaaggaag
    15181 cccgagaaca ggatcgacac caccatagtc aggctgaact gctggtagat gatgccgacc
    15241 gcgccgggaa agaacgccat cggaacgaat acggccgtca gcaccagcgt gatgccgatg
    15301 atggcaccgg tgatctgccc catggccttg cgggtggcct cgcgcggcgg cagcccctcc
    15361 tcggacatga tgcgctcgac gttctcgacc accacgatcg cgtcgtcgac gaggatgccg
    15421 atggccagca ccatggcgaa catcgtcagc acgttgatcg agaagcccgt cgccagcatc
    15481 acggcgcacg tgcccagcag cgccaccggc accacgatcg tcggaatgat cgtgtagcgg
    15541 aagctctgca agaagagcag catcacgaga aagacgagcc ctaccgcctc gagaagcgtg
    15601 tgcagcacct tctcgatgga gatctccacg aacggggagg tgtcgtaagg aatgctgtac
    15661 tcgaggcccg gcgggaagaa gcgggacagc tcctccatct tggcgtggat cgccttcgac
    15721 gtggcgagcg cgttgcccgt cggcgaaagc tgcacgccga t
//
LOCUS       DASVDU010000053        11465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1283262, whole genome shotgun sequence.
ACCESSION   DASVDU010000053 DASVDU010000000
VERSION     DASVDU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283262"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF226_03675"
     CDS             complement(<1..428)
                     /locus_tag="VF226_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073117.1"
                     /translation="MRAWRVSLGMGAILVIAGLATVSTWAQHPARLTHPQPYAGQQNR
                     AISSFSEEELADLREGRGMGLARPAELNGYPGPAHVLELATELELTDEQRRSVEAIFS
                     RMKKAAQAAGAEYLEAERAVDAAFRSGRTSPEEIRALVRRA"
     gene            complement(434..724)
                     /locus_tag="VF226_03680"
     CDS             complement(434..724)
                     /locus_tag="VF226_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7073118.1"
                     /translation="MTAARAVPTVQIDNDRVRVTEWRFAPGAETGFHVHEMDYVVVPS
                     ADGRLKLVSPEGEETYADLKRGVSYFRRAGVHHNVINASGGEYAFVEIELKG"
     gene            complement(721..2421)
                     /locus_tag="VF226_03685"
     CDS             complement(721..2421)
                     /locus_tag="VF226_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX7073119.1"
                     /translation="MSNSGPRGGRRNHPGSGRRKAPMFPKGRVLRQEEVEAVRQLIGP
                     GPYERALLIEYLHKIQDAEHCLPAGHLHGLAELMRIPMAEVYEVATFYAHFDVVNDGE
                     ARPPAVTIRVCDSLSCMLAGAESLLKALQQESLPGVRVVRAPCMGSCHTAPALEVGHK
                     HVDHATVDKVRALLAAGDTHPDIPPYQDFEAYREDGGYKILASCLSGERKVEDVIATL
                     SDGGLRGLGGAGFPTGRKWSLVRAEKAPRLMAVNGDEGEPGTFKDRHYLERRPHQFLE
                     GMLIGAWAVEAAEVFIYMRDEYPAVLEILRREVPKLEAAGLTRHTKVHIRRGAGAYIC
                     GEESAMIESIEGKRGLPRHRPPYVAQVGIFGRPTLVNNVETLYWVPEILAKGADWFAS
                     QGKNGAKGFRSYSVSGRVKNPGVVLAPAGSTARDLIELCGGMADGHTFKGYLPGGASG
                     GILPASMADIPLDFGQLEKYGCFVGSHAVVILSDKDDMKAVALNLMKFFKDESCGQCT
                     PCRNGTEKAVMLMSEPSWNKEVLDDLAQVMADASICGLGQAAPNPLRSVFKFFPEDLK
                     "
     gene            complement(2434..3309)
                     /gene="sucD"
                     /locus_tag="VF226_03690"
     CDS             complement(2434..3309)
                     /gene="sucD"
                     /locus_tag="VF226_03690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7073120.1"
                     /translation="MAVLANRDTRVICQGLTGRAATYHVGRMIAYGTRLVAGVTPGRG
                     GQRHLDVPVFDTVAEAAAETGATASLVFVPPANAADAMIEAIDAGIELVVCVTERVPV
                     MDMIRVHQKLAGSRTRLVGPNSQGVLVPGETQLGVMATINARRGPVGIASRSASLTSE
                     VVAQTTAAGLGQSTTIGVGGDPVHGIGLAESVRLFLDDPATEGIILIGEIGGNDEEEA
                     AALIKRLAPDKPIVALVVGRHAPAQQRMGHAGALVAGQAGSAEAKMEALREAGVLIAP
                     RADLVGMTMRQALVG"
     gene            complement(3309..4466)
                     /locus_tag="VF226_03695"
     CDS             complement(3309..4466)
                     /locus_tag="VF226_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7073121.1"
                     /translation="MNLHEFQAKELLAQFGVPIPRGRIAASPAEAASVARRLGFAQCV
                     VKAQVNAGDRAANGGIRFASSVDEAGRMAAELIGSPLVTAQTGPAGKLVRWVYVEEAV
                     AFKRELYVAVVVDRTAGALVLVTRDRDAGPDAGMTTRLELSEAGVRGDFEKAAAAFRL
                     EPSLTSLLAKRMSQIAQAAVALDAMLVEISPLVLTHSGEFIALDAKMSADHNALFRHP
                     DLIGLREVSEAEDGDPIALAADLHQINYMRLDGDIGLVVNGAGLALATLDLLHAAGGR
                     PANFMDIRTTASTLDIAYAFELIAANPRVGAVLVNVHGGGMQRCDTIADGIGIAVRRR
                     ACPAPIVVRLAGNNAEFAASRFRSYGVPVIETADMWEAANTAVQLATRKAA"
     gene            4856..5626
                     /locus_tag="VF226_03700"
     CDS             4856..5626
                     /locus_tag="VF226_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7073122.1"
                     /translation="MELLVAAAAVGDQFASYVQELLGFDAALIVNILRIVGIDIVLAG
                     DNAVVIALACRSLPPRQRMTGIILGAGVAVLLRIFFTVLIQFLLEVQWLKLIGGGLLF
                     WIAVKLLTSEEGHEGSVKSGTSLFEAVRIVAIADLVMSLDNMLAIAAAAGGHTYLIIF
                     GLALSIPLVVAGSTLIMTLLTRFPILVWGGAALLGWISGELIATEPVLRPFFETLGQS
                     LHTTPKTISRVLEVIGALAVILTGWLITQRAGRKIGHG"
     gene            complement(6058..6576)
                     /locus_tag="VF226_03705"
     CDS             complement(6058..6576)
                     /locus_tag="VF226_03705"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7073123.1"
                     /translation="MHGNDSSDDQESVVSNRTMDIVVALLLILVSAIVIYDSARLGFG
                     WRENEGPASGYFPFYIAVIMAFASLMNLLRAVLARDGAGSGSFVSKPALMRVLAVLIP
                     SFIFVWVIQLLGLYVAAAIFITAFMLTLGRESIFRSLLIGISIPVLLFILFEIWFLVP
                     LPKGPVENFLGY"
     gene            complement(6646..7602)
                     /locus_tag="VF226_03710"
     CDS             complement(6646..7602)
                     /locus_tag="VF226_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7073124.1"
                     /translation="MTAPPALAWEPTKAVTFVIPAGTGGGADQMARFIQGVVSKHKLM
                     DQPIVVINKSGGAGAEGFLEVKNSKGDPHKIIITLSNLFTTPLSTGTPFSWKDLTPVA
                     MLALDQFILWVPADAPYNTAKEYVEAVKAGADRQYKMGGTGSKQEDQIITVAIERQTG
                     GKKFTYVPFQGGGTVAVQLVGKHINSSVNNPIEAVQHWKSGALKPLCIFDSKRSTYNE
                     KVTSDKAWSDIPTCMEAGLPVEYQMLRGIFMPGGVTEDQVKFYVDLFQKVRETQEWKD
                     FMKNGAFNQTFMSGEEFKKWVENAEKVHYDLMKEAGFLAAGK"
     gene            complement(7756..9261)
                     /locus_tag="VF226_03715"
     CDS             complement(7756..9261)
                     /locus_tag="VF226_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7073125.1"
                     /translation="MEEIASLMQGFAVVLTPFNLLLMLVGILLGVIIGVLPGLGGANG
                     VAILLPLTFSMPPTSAIIMLSCIYWGALFGGAITSILFNIPGEPWSVATTFDGHPMAQ
                     KGKADEALTAAFTSSFVGAMVAVIVITFLAPLVASFALQFGPAEFFAVQFLTFASFIG
                     MGRGSPFKTLASMCLGFALASVGLDNVTGQLRMTFGFESLLNGFVFLVAVIGLFGIGE
                     ILSSMEEGLSFSGAQAKIRMNAVLQTWKRFPRLWFTFLRSCVIGCWMGITPGGATPAS
                     FMSYGLAKQFSKNSSNFGKGEIEGVVAPETAAHAAGTSALLPMITLGIPGSPTAAVLL
                     GGLMIWGLQPGPMLFIEQPDFVWGLIASMYLGNVVGLMIILTCVPLFAAILRIPFSII
                     APIILVICAVGAYTVNNSMFDVWMMVVFGVVGYVFVKLGYPLPPLVLALVLGDMAEGS
                     FRQAMLLSQGDLSIFFSNWLVSSIMGLGLILLFWPGIRMLIAFAKPKPQHA"
     gene            9596..10282
                     /locus_tag="VF226_03720"
     CDS             9596..10282
                     /locus_tag="VF226_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073126.1"
                     /translation="MASTQIRITPLGEAQSLKTRAYEALKSAILNMNIYDSNAELRLD
                     ERQLSAQFGVSRTPLREALAQLDQEGLVRIVARRGIYIVRKTKAEIMEMITVWAALES
                     MAARLATKEATDSEIASLHDLIDAFSTDEVARKMGEYSDANIRFHQAIIGLGKCELIR
                     ELTDKLFFHVRAIRQRTIFEQDRARRSVVDHKEIVAALEARDTERAERLVREHTLRLR
                     DHVERYVDLD"
     gene            10317..>11465
                     /gene="oxc"
                     /locus_tag="VF226_03725"
     CDS             10317..>11465
                     /gene="oxc"
                     /locus_tag="VF226_03725"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX7073127.1"
                     /translation="MSATAQGIEAQAGAQEQELTDGFHLVIDALKLNGINTIYGVPGI
                     PITDLGRLAQAKGMRVISFRHEQNAGNAAAIAGFLTKKPGVCLTVSAPGFLNGLTALA
                     NATTNCFPMILMSGSSEREIVDLQQGDYEEMDQLAIAKPLCKAAFRVLHAQDIGIAVA
                     RAIRAAVSGRPGGVYLDLPAKLFPQVMTAEAGAKSLVKVIDPAPAQIPAPSAVNRALD
                     VLKSAKRPLIILGKGAAYAQADDEIRTLIEKTGIPFLPMSMAKGILPDTHPQCAGAAR
                     SLVLKECDVAMLIGARLNWLLSHGKGKTWGNAPKKFIQIDIEPREMDSNVEIAAPVVG
                     DIGSCVSALLGGIGANWPAPPADWINAVKAKKEENIAKMAPKLMKNTAP"
BASE COUNT         2210 a         3693 c         3434 g         2128 t
ORIGIN      
        1 gcccgtctca ccagggcgcg aatctcctcg gggctcgttc taccggagcg gaacgcggca
       61 tcgacggcgc gctctgcctc cagatactca gcaccagcgg cctgcgccgc tttcttcata
      121 cgcgagaaaa tcgcctcgac cgatctgcgc tgctcatcgg tcaactccag ctccgtcgcc
      181 agctcgagca cgtgcgccgg ccccggatag ccattgagct cggccgggcg cgccagtccc
      241 atgccgcggc cttcgcgaag atcggcgagt tcctcctccg agaagctgga tattgcccgg
      301 ttctgctggc cggcataggg ctgcggatgc gtcagcctgg ctggatgctg ggcccacgtg
      361 gaaaccgttg cgagaccggc gataaccagt atcgcgccca ttcccaagct cacccgccaa
      421 gctctcatcg accctatccc ttgagctcga tctcgacgaa agcgtattca ccgccgctgg
      481 cgttgatcac gttgtggtgg acgcccgccc ggcggaaata ggaaacaccg cgcttcaggt
      541 cagcgtaggt ctcctcgccc tccggggaaa ccagcttcag ccgcccatcc gccgacggca
      601 cgacgacata atccatctcg tgcacgtgga agcccgtctc ggccccgggc gcgaaccgcc
      661 attcggtcac acggacccga tcgttgtcga tctgcaccgt gggcacggcc cgggctgccg
      721 tcatttcaaa tcctccggga agaatttgaa gacggaccgc agcggattgg gtgctgcctg
      781 cccaagcccg cagatcgagg cgtcggccat cacctgcgcg agatcgtcga gcacctcctt
      841 gttccagctc ggctcggaca tgagcatcac ggccttttcc gtcccgttgc ggcacggcgt
      901 gcattgcccg cagctctcgt ccttgaaaaa cttcatgaga ttgagcgcga ccgccttcat
      961 gtcgtccttg tcggaaagaa tgaccaccgc gtggcttccg acgaagcagc cgtatttctc
     1021 gagttgtccg aaatcgagcg ggatatcggc catggatgcg ggaaggatgc cgcccgacgc
     1081 gccgcccggc agatagccct tgaacgtgtg cccgtcggcc atcccaccgc acaattcaat
     1141 caaatcccgc gcagtcgagc ccgcaggtgc aagcaccacg ccggggttct tcactcgtcc
     1201 ggaaacggag taagagcgga agccttttgc accattcttg ccctgtgacg cgaaccaatc
     1261 cgcgcctttg gcgagaattt ctggcaccca gtaaagcgtc tcgacattgt tgacgagcgt
     1321 cgggcggccg aagatgccca cctgcgccac ataaggcggc cggtgccgcg gcaagccgcg
     1381 cttgccctcg atcgactcga tcatcgcgct ttcttcgccg cagatatagg cgcccgcgcc
     1441 gcggcggatg tgcaccttcg tgtggcgcgt caggcccgcg gcctcgagct tcggcacctc
     1501 gcgacgcagg atctcgagca ccgccgggta ttcgtcgcgc atgtagatga agacctcggc
     1561 cgcttcgacc gcccacgcgc cgatcagcat gccttcgagg aactgatgcg ggcggcgctc
     1621 gaggtaatgc cggtccttga acgtgcccgg ctcgccctcg tcgccgttga cggccatcag
     1681 ccgcggcgcc ttctcggcgc gcacgaggct ccatttgcgc cccgttggga acccggcgcc
     1741 gccaaggccg cgcaagccgc cgtcggagag cgtcgcgatc acgtcctcga ccttgcgttc
     1801 gccggacaga caggacgcca ggatcttgta tccaccatcc tcccgatagg cctcgaagtc
     1861 ctggtacggc ggaatatcag gatgcgtgtc gccggcggcc agcagcgccc ggaccttgtc
     1921 cacggtggca tggtcgacat gcttgtggcc cacttcgagc gcaggagccg tgtggcaaga
     1981 gcccatgcac ggcgcccgca cgacgcgcac cccgggcagc gattcctgtt gaagcgcctt
     2041 cagcagtgat tcggcgcccg ccagcatgca cgacaggctg tcacagacac ggatcgtcac
     2101 cgcgggcggc cgcgcctcac cgtcgttgac cacatcgaag tgcgcgtaaa acgtcgcgac
     2161 ctcgtacacc tccgccatgg ggatgcgcat cagctcggcc agcccgtgca ggtgaccggc
     2221 gggcaggcag tgctccgcat cctggatctt gtgcaggtat tcgatcagca gtgcgcgctc
     2281 gtaaggacca ggaccgatca gctggcgaac ggcctcgacc tcctcctggc gcagcacgcg
     2341 gcctttcggg aacatcggcg ccttgcgccg gccggaaccg ggatggttcc gccggccacc
     2401 gcgcggccct gagttgctca tatcgttcat cgttcagccc accaaagctt gccgcatcgt
     2461 catgcccacg aggtcggccc gcggcgcgat cagcacgcca gcctcccgca atgcttccat
     2521 cttggcctca gcgctgcccg cttgaccagc cacgagggcg ccagcgtgcc ccattcgctg
     2581 ctgcgccggc gcatgtctgc cgaccacgag cgctacgatc ggtttgtcgg gcgccagcct
     2641 tttgatcaaa gcggccgcct cttcctcgtc atttccgccg atctcaccga tcaggatgat
     2701 accctcggtg gcgggatcgt cgagaaaaag ccgaacgctc tctgcgagcc cgatgccgtg
     2761 cacgggatcc cccccgacgc cgatcgtcgt cgattgaccg agccctgcgg ctgtcgtctg
     2821 tgcaacgacc tcgctggtca gagaggccga acgtgacgcg atcccgacgg gcccgcgtcg
     2881 ggcattgatg gtcgccatca cgccgagctg cgtctcgccg ggcacgagga ccccctgcga
     2941 gttcggaccg acgagccgcg tgcgggagcc cgccagcttc tggtgaacgc gaatcatgtc
     3001 catgacgggc acgcgctcgg tgacgcagac gacgagctcg atgccggcat cgatggcctc
     3061 gatcatcgca tcggcagcgt tggcgggcgg cacgaacacg aggctcgcgg ttgcgcccgt
     3121 ttcggcggcc gcttcggcca cggtatcgaa cacgggcaca tcgagatggc gctgcccgcc
     3181 cctgccgggc gtcacaccgg ccacgagccg cgtgccgtag gcgatcatgc gtccgacgtg
     3241 gtacgtcgct gcccggcctg tcaggccctg gcaaatgacg cgcgtgtctc tgttcgcgag
     3301 gaccgccatc acgccgcctt ccgggttgcg agttgcacgg cggtattcgc cgcttcccac
     3361 atgtcggccg tctcaatgac aggaacacca tagctcctga agcggctcgc ggcgaattcg
     3421 gcattgttgc cggcaagccg gacgacaatc ggtgccgggc atgcccgccg gcgcacggcg
     3481 atgccgatcc cgtccgcgat ggtatcgcag cgctgcatgc cgccgccatg cacgttgacc
     3541 agcaccgccc cgacgcgcgg gttggctgcg atcagctcga aggcataagc gatgtcgagt
     3601 gtcgaggcgg tggtgcggat atccatgaag ttggccggcc ggccgcctgc cgcgtgcaga
     3661 agatcgagcg tcgcgagcgc cagcccggcc ccgttgacga ccagcccgat atcaccgtcg
     3721 agccgcatgt agttgatctg atgaaggtcc gcggccagcg cgatgggatc gccgtcctcc
     3781 gcctcgctca cttcgcgcag cccgatgaga tcaggatggc ggaagagtgc gttgtggtcc
     3841 gcgctcattt tggcatcgag cgcgatgaac tcgccggaat gtgttagaac gagagggctg
     3901 atctcgacca gcatggcatc gagcgccacc gccgcttgcg cgatttgcga catccgcttc
     3961 gccagcagcg acgtaaggga tggctcaagc cggaaagccg ctgcggcctt ttcgaaatcc
     4021 cctctgaccc ctgcttcaga caactcgagg cgcgtggtca tcccggcatc cgggccggcg
     4081 tcgcggtccc gcgtgacaag aaccagcgcg ccggcggtcc tgtcgaccac gacggcgacg
     4141 tagagttcac gcttgaaagc gacggcttcc tccacgtaaa cccagcgcac caactttccg
     4201 gccggccctg tctgggccgt gacgagcggt gagccgatca gctcggctgc cattctcccg
     4261 gcttcatcga cgctagaggc aaaacgaatg ccgccgttgg ccgcgcgatc accggcattt
     4321 acctgcgcct tcaccacgca ctgcgcaaac cccaggcgcc gcgccacgga tgcggcctct
     4381 gccggactcg cagcgatccg cccacgcgga atgggcacgc cgaactgggc cagaagctcc
     4441 ttggcctgga attcgtgcag gttcatggac cgctcctcct gcaatccggt ccgtctgccg
     4501 gccagccccg gcctattccg cttctcgatc tttcactccg ggcggccaac tgcagcgccc
     4561 tttcgcgatg tgtcgccttg caatccgttc cgcaacggac gaatacggtc gccgaggcac
     4621 tggcatacca aatacaatat gccaattgcg tagcgatgtc gagcatgcga ccatggattt
     4681 gcagacgggg gacgttgctc agctttgaat gcgggtgctg ccttgcgcac tcttcacggc
     4741 cgaacagaga ttgtgccgag agagctgcga tccccgatcg cacgcgaagc atgggatgac
     4801 agcgcgttct tgaaggggct ggcgcgaggg aaggtctgcg ccacaggaga atgggatgga
     4861 attgctagtt gcggcagccg cggtcggaga tcaattcgcc agctatgttc aggagctgct
     4921 cgggttcgat gctgcgctca tcgtgaacat cctgcgcatc gtcggcatcg acatcgtcct
     4981 cgccggtgac aacgccgttg tcatcgcgct cgcgtgccga tctctcccgc ctcgacagcg
     5041 aatgactggc atcattttgg gcgccggtgt tgcggtcctg ctgcgcatct tcttcaccgt
     5101 tcttattcaa ttccttctcg aggtgcagtg gttgaagctg atcggcggcg gtctcctgtt
     5161 ctggattgcc gtcaagctgc tcacgtcgga ggaaggccat gaaggcagcg taaagagcgg
     5221 gaccagcctg ttcgaggccg ttcgcatcgt cgcgattgct gatctcgtga tgagtctgga
     5281 caatatgctc gcgattgcgg ctgcagccgg cggccatacg tatctcataa tttttggctt
     5341 ggcactcagc attcccctgg tcgttgctgg atcgacgctc atcatgaccc tgctgacccg
     5401 cttcccgatt ctcgtctggg gtggtgcggc gctgctcggc tggatttccg gcgagttgat
     5461 cgcgacggaa cccgtcctgc gccccttctt cgaaaccctt ggacagagcc tgcacacgac
     5521 accgaagacc atctcgcgtg tcctggaggt gattggcgca ctggcagtca ttctgaccgg
     5581 ctggctgatc acgcagcgcg ctggtcgaaa aatcggccac ggctgagtct gcttccagct
     5641 gggttttggc tgcccgaccc cgagcatatg cttgctcggg gatcacgtgc cggggccatg
     5701 cccgaggtcg aaatggggga gtggtagcac tatcggaaga atgggagctc agctggcaac
     5761 tctggcctgc tccagccgaa atcggaaacc tcgaggatct cggcgtcgat cgcgtggctc
     5821 cccagcgcct ctcaagcgcc gctcgggccg gcgctggcat gagcacatct gagccggcgc
     5881 aagtcctttc gccattgccg aaatcgccgc cccgcgcagg ccttccggct tcagtgtcag
     5941 cacgcaagtt ctgagcaggc gaaagcggga ggccctgccg gctcaggtct tcatccggca
     6001 ctcccccggc cgcagttcag cgccgaaccg caacgccgaa gagcatcgaa attgtgatca
     6061 atagccgagg aaattctcca ccggaccctt tggcagcggc accaggaacc atatttcgaa
     6121 gagtatgaag aggaggaccg gaatcgaaat gccgatgaga agcgatctga aaatcgactc
     6181 gcgccccagt gtcagcatga atgcggtgat gaaaatcgcc gcagcaacgt aaagcccgag
     6241 aagctggatc acccaaacga agatgaagga cggtatcaag accgcgagga cgcgcatcag
     6301 cgccggcttc gagacgaagg atccgctgcc cgcgccgtcc cgcgcgagaa cggcgcgcag
     6361 caaattcatc aagctggcga acgccatgat cacggcgatg taaaacggga agtatccgga
     6421 cgcaggcccc tcgttttcac gccaaccaaa tccaagtcgc gcactgtcat aaatcacgat
     6481 tgcgctgacc agaatcagga gcagcgcgac gacgatatcc atcgtcctgt tagagacgac
     6541 gctctcttga tcgtccgaac tgtcgttgcc gtgcatcggg tgccccgggg aatcgatctg
     6601 aaagcagaag gcagaccgga agttccggtc tgccttgtat ggttttcact tgccagcggc
     6661 gaggaagccc gcctctttca taaggtcata gtggaccttc tcggcattct cgacccactt
     6721 cttgaattct tcgccgctca tgaatgtctg gttgaaggca ccattcttca tgaagtcctt
     6781 ccattcttgg gtttccctca ccttctggaa gagatcgacg tagaatttga cctggtcttc
     6841 ggtcacaccg cccggcatga aaatgccgcg cagcatttgg tattctaccg gcaggcccgc
     6901 ctccatgcag gtcggaatgt cactccacgc cttgtcgctc gtcaccttct cgttataggt
     6961 cgaccgcttg ctgtcgaaga tgcagagcgg cttgagagca cccgatttcc aatgctggac
     7021 ggcctcgatc ggattgttga ccgacgagtt gatgtgtttt cctacgagct ggacagcaac
     7081 cgtgccgcca ccctggaacg gtacgtaggt gaacttctta ccgccggtct gccgctcgat
     7141 ggcgaccgtg atgatctgat cctcctgctt ggaaccagtg ccacccatct tgtactgcct
     7201 gtctgcacca gccttcacgg cctcgacgta ctctttggcc gtattgtatg gagcatccgc
     7261 aggcacccag agaatgaact ggtcgagcgc cagcatggca acgggtgtca ggtccttcca
     7321 actgaacggt gtccctgtgc tcagaggcgt ggtgaacagg ttcgacagtg tgatgatgat
     7381 cttatgtggg tcgcctttgc tgttcttgac ctcgaggaag ccctccgctc cggctccacc
     7441 cgacttgttg atcaccacga tgggctggtc catcagctta tgcttgctga cgactccttg
     7501 gatgaacctg gccatctgat cagcaccgcc gccagttccc gcagggatga cgaaggtgac
     7561 cgccttggtc ggctcccatg ccaaagcagg tggtgccgtc atggcgaggg cggctgccaa
     7621 gccacatgct accgttagtg ccttattcag cttgcgcatc tccagctcct ttttcgtttc
     7681 ctcgaaaaat ttctgattgc ttttggaacc ctcgaggggt tcatccttca aagtacaccc
     7741 ttcctccggc gattctcacg cgtgttgcgg ttttggtttc gcgaaggcga tcagcatcct
     7801 gatccctggc cagaacagca ggatgaggcc cagccccatg atgctgctga cgagccaatt
     7861 ggagaagaat atcgatagat ctccctggct cagaagcatg gcctgccgaa acgagccttc
     7921 ggccatatcg ccgagcacca gcgccaggac gaggggcggc agcgggtagc cgagcttcac
     7981 aaatacgtat cccaccaccc cgaaaacaac catcatccaa acgtcgaaca tggagttgtt
     8041 caccgtgtag gcgccgaccg cgcagatgac gagaatgatg ggcgcgatga tcgagaacgg
     8101 gattcgcaga attgcggcaa acaagggcac gcacgtcaag atgatcatga gcccgacgac
     8161 attgccgaga tacatggatg cgatcaatcc ccagacgaaa tcgggctgct cgatgaacag
     8221 catcggacca ggttggaggc cccagatcat gagccctccg agcagcacgg ccgcggtcgg
     8281 tgagccagga atgccgagcg tgatcatcgg gagaagtgcg ctcgtccccg cagcatgggc
     8341 cgcggtttcc ggtgccacga ccccttcgat ctctcctttc ccgaaattcg aactgttctt
     8401 tgaaaattgt ttggccagcc cgtagctcat gaacgaggca ggcgtggcgc cgcccggagt
     8461 aattcccatc cagcagccaa tcacgcagct gcgcaagaat gtgaaccaga gcctggggaa
     8521 ccgcttccac gtctgcagga cagcattcat tcggatcttg gcctgggcgc cggagaagct
     8581 caaaccttct tccattgacg agaggatctc gccgatcccg aacagaccga tgaccgcgac
     8641 gaggaatacg aacccgttaa ggagcgactc gaaaccgaac gtcatccgca gctgcccggt
     8701 cacgttgtcg agcccgaccg aggccagcgc gaaaccgagg cacatggatg caagtgtctt
     8761 gaatggcgag cctctgccca ttccaatgaa gctggcgaac gtcaggaatt gaactgcgaa
     8821 aaattcggcc gggccgaact gcagtgcaaa gcttgcaacg agcggggcga gaaaggtgat
     8881 gacgatcacc gccaccatcg cgcccacgaa cgacgacgtg aatgccgccg tcaaagcctc
     8941 gtcggccttt cctttctggg ccatggggtg cccgtcgaat gtcgtggcga cggaccacgg
     9001 ctctccagga atattgaaca gaatggacgt tattgcgccc ccgaacagcg ccccccaata
     9061 aatacaactc aacatgatga tcgccgaggt cggcggcatc gagaatgtta gtggaagaag
     9121 gattgcgacg ccattggcgc cgccgaggcc gggcaggact ccgatgatga cgccaaggag
     9181 aatgcccacg agcatcaaga gcaggttgaa cggcgtcaga acgacggcga agccctgcat
     9241 aagcgaggcg atttcttcca acggacgatt tcctctctgt catttccaag caggattctc
     9301 gaaaccctgc ttgctcgcaa cgtgcgcatt cgttgcggat tttggtatac catatacccg
     9361 cgatcgagcg atgcgcaatc ccggattcgt tgctttcagg tttgctgttt tctcatgacg
     9421 gtcgaatttg acatgacgat cgaactgtga accgctcgcc tgtttcagct cggcccagag
     9481 cgatcgtgtg tgccgcactt gcctgcgaca ggctgtctgg tgtatggtat accagagata
     9541 acggatgcgc cgtgctccgg aaggagaacg gctttcaccc tattcaggca ttcggatggc
     9601 cagtactcag atcaggatca cgcctctcgg agaagcccag agtctcaaga ctcgggccta
     9661 cgaggcgttg aagtctgcca tcctcaacat gaacatttat gacagcaacg ccgagctgcg
     9721 tctcgacgag cgccagctct cggcgcagtt cggcgtgagc cgtacgccac tgagagaggc
     9781 gttggcccag ctcgaccagg agggattggt ccgtatcgtt gcgcggcgcg gcatttacat
     9841 cgtacgcaag acgaaggccg agatcatgga gatgatcacg gtctgggcgg cgctcgagag
     9901 catggcggca cgcctggcca ccaaggaagc caccgacagc gagatcgcaa gcctgcacga
     9961 cctgatcgac gcgttctcca cggacgaggt cgcccgaaag atgggcgagt actcggacgc
    10021 gaacatccgc tttcaccagg cgatcatcgg gctcggcaag tgcgagctga tccgcgagct
    10081 cacggacaag ctgttctttc acgttcgggc cattcggcag cggactatct tcgagcagga
    10141 tcgtgcacgg cgttcagtgg tcgatcacaa ggagatcgtc gccgcgctcg aggcacgcga
    10201 tacggaacga gccgaacggc tggtgcgcga gcatacgctg cggctcagag atcacgttga
    10261 acgctacgtc gacctcgact agcgcccccc ggaacaggac ctgaggaagg agcgaaatgt
    10321 ccgcgacagc gcaaggcatc gaggcacagg caggtgccca ggagcaagag ctgacggatg
    10381 gctttcatct cgtcatcgac gccctcaagc tcaacggcat caacacgatc tacggtgtgc
    10441 ctggaatccc gatcacggat ctgggccgct tggcgcaggc taaaggcatg cgggtcatct
    10501 cgttccggca cgagcagaac gccggcaatg cggccgccat cgccggcttc ctgacgaaga
    10561 agcccggcgt ctgcctcacc gtttcggcgc ccggctttct caacggcctg accgcgctcg
    10621 cgaacgccac gaccaactgc ttcccgatga tcctgatgag cggctcgtcc gagcgcgaga
    10681 ttgtcgacct gcagcagggc gactacgaag agatggatca gctcgcgatc gccaagccgc
    10741 tgtgcaaggc agcctttcgc gtgctccacg cgcaggatat cggcatcgcc gttgcgcgtg
    10801 ccatccgcgc ggcggtctcg gggcgcccgg gcggcgtcta cctcgatctg ccggccaagc
    10861 tgttcccgca ggtgatgacg gccgaggccg gggccaaatc gctggtcaag gtgatcgatc
    10921 cggcaccggc gcagatcccc gctccatccg ccgtcaatcg cgcgctcgat gttctgaaaa
    10981 gcgccaagcg tcccctcatc attctgggta aaggcgcggc ctacgcgcag gcggatgacg
    11041 aaatccgcac cctcatcgag aaaactggga tccccttcct gccgatgagc atggcgaagg
    11101 gcattctgcc ggatacgcat ccgcagtgcg cgggtgcggc gcgctcactg gtgctcaaag
    11161 aatgcgacgt cgcgatgctc atcggtgctc gcctcaactg gctgctgtcg cacggcaaag
    11221 gcaagacctg gggcaacgca ccgaagaagt tcatccagat cgacatcgaa ccccgggaga
    11281 tggacagcaa cgtcgagatc gccgcgcccg tcgtcggcga cattggctcg tgcgtctccg
    11341 ctctgctcgg cggcatcggc gccaactggc ccgcgccgcc cgcggactgg atcaacgccg
    11401 tcaaggcgaa gaaagaggaa aacatcgcca aaatggcgcc gaagctgatg aagaacacgg
    11461 cgccc
//
LOCUS       DASVDU010000054         8624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_507709, whole genome shotgun sequence.
ACCESSION   DASVDU010000054 DASVDU010000000
VERSION     DASVDU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507709"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VF226_03730"
     CDS             <1..663
                     /locus_tag="VF226_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7073128.1"
                     /translation="HSAHDRLAFHRHESAYIALPLEGSYEESSADGRFACAPGIAVVH
                     GEWHMHADRFTGAGGRVLNIDLPSGAVPPGGSAAVTVDFVEIEKLARRQPARAAAAVL
                     ETWASAGDPAGAGSGLVYAFLRSVRLSPAARIAELAAELGVSREHLARRVKAHLGIGP
                     SRLRSEWRFRCAAELLARGESLARVAVAAGYSDQSHMTRDIKARLGRTPGRLGRLSRP
                     RR"
     gene            complement(644..1426)
                     /locus_tag="VF226_03735"
     CDS             complement(644..1426)
                     /locus_tag="VF226_03735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7073129.1"
                     /translation="MHEAVATRRFFEISGTRSRLVPAIVAIVVAGLLMEGMLRLGRVP
                     ARWLYENGPPSWDDRAWIYVGLAILFQAILGLIGIGVMKTVLPRADAHLRWPPGRTLA
                     GLGFVIGIGMALVMLVADYWPQLLLGEPFDTLYATNPVDATGWLVSMAITGLAEETIF
                     RGLLVGGLVLFVTGRVRAGRFDVSLAGVVVALLFCVAHWRTFVVEPLHMAVAQQSYAF
                     AWGLVYVWLMERSRSLLAPIIAHGTGNAIEVGLVMLLSVAAG"
     gene            complement(1734..2567)
                     /locus_tag="VF226_03740"
     CDS             complement(1734..2567)
                     /locus_tag="VF226_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073130.1"
                     /translation="MKSRVFRRLAALSVTALGGLLLALDANAGDNVVPEHPMMSDRFF
                     VGAGAAWVQSNVTANANRGRIGLGSFIDFEDDVGLEKSNLLGMFDFRMRLSERWLLEA
                     EYFRLDRSGERELSRTVDWGDLNIPVNALVSGSSDLEDLRISVGYSFFRRKDKEVGFG
                     LGAHVMSFDAELSTRNLGSERASQSAPLPVLTMYAQFALTNRWLLRVRVDRLSLDTGE
                     VDGSIFSSATDFVYQPWRHFGLGLGYRDINMTISSTSESWRGKAQVRHTGPYLFISGS
                     F"
     gene            complement(2595..2723)
                     /locus_tag="VF226_03745"
                     /pseudo
     CDS             complement(2595..2723)
                     /locus_tag="VF226_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596751.1"
                     /note="this stereospecific enzymes reduces the R isomer of
                     methionine sulfoxide while MsrA reduces the S form;
                     provides protection against oxidative stress; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
     gene            3114..4148
                     /locus_tag="VF226_03750"
     CDS             3114..4148
                     /locus_tag="VF226_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEX7073131.1"
                     /translation="MKRNILLATVVAVMLTHAASAQDRLMQSAQGLFEPLPTEPPDIG
                     IRSTPERIALGKMLYFDPRLSESHSISCNSCHLIGLGGVDLQETSIGHRWQHGARNAP
                     TVLNAVFNTAQFWDGRAGTLDEQAGGPLVNPIEMGTTEEHVVLQLEGIPGYVDAFAKA
                     FAEADQPVTFENIRDSIALFEATLITPNAPFDRYLRGDAQAMTDDQKTGLQLFIEKGC
                     TGCHHGMNVGGNMYAPFGVVERPGAALLPPTDKGRFAVTKTVSDEYVFKVPSLRNIAL
                     TPPYFHSGRVWDLRQAVAVMGTSQLGTPLSDDEVVQITAFLHALTGDQPVMTLPLLPP
                     SVAATPRPNP"
     gene            4378..4980
                     /locus_tag="VF226_03755"
     CDS             4378..4980
                     /locus_tag="VF226_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7073132.1"
                     /translation="MEDTHGSIEALPLPGAAASMRMWQLDDSRPADEAAFRKELLKSL
                     PKLRRFAVNHCRSKADTDDLVQLTCERALVHWRQWSGCGSLDRWLISILVNACRDEAR
                     ARAGKALYALDAIPEIAAGSSTVDSAYLQQIQGEVLRIPDLFRDVLWLVGCEGLSYRE
                     TAEILHVPIGTVMSRLNRARHLLQARLAGRGQAAAAGPPH"
     gene            complement(5292..5552)
                     /locus_tag="VF226_03760"
     CDS             complement(5292..5552)
                     /locus_tag="VF226_03760"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7073133.1"
                     /translation="MFEDLGFAKGEAENLRLRADLMRQLEKVIRDSGVTQSEAAELLG
                     IQQSRVSDLLRGKIGRFSIDMLVKLLARTGRGVEVKVKRRVA"
     gene            5810..5947
                     /locus_tag="VF226_03765"
     CDS             5810..5947
                     /locus_tag="VF226_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073134.1"
                     /translation="MAATVFVDTDIFVYSRDASEPKKQSVAARWLEELWIEQACRAGS
                     Y"
     gene            6052..6288
                     /locus_tag="VF226_03770"
     CDS             6052..6288
                     /locus_tag="VF226_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073135.1"
                     /translation="MLEETGCELLVFGPDLAEWAAALGAEIPALACHGLGARLDDGCD
                     LLADAENASDTAPAIDVSGDDVILTRLLRALPAH"
     gene            6285..7298
                     /locus_tag="VF226_03775"
     CDS             6285..7298
                     /locus_tag="VF226_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7073136.1"
                     /translation="MKAAQHTQGSYAAICRNVLMNLLPVEHDDAMLHAASLIHASGVF
                     VLPFWLRGARTVIMPGFEPGLFLELIARERITAVNVVPTMLQMLLDHPAFATIDVSSL
                     RQVIYGASPMPRPVIERAIDLWGRERFWQYYGQTECPLAIAVLRPEDHEEGLLGACGK
                     PAIDVEVRLVDESGADVAAGEPGEIAVRGPSTMAGYYNAPALNREMFLDGGWLRTRDV
                     GQFDERGFLHLKDRTSDMIVTGGYNVYPREIEDVLATHPAVAECVVVGVADTKWVEAV
                     TAVVALRPQAQVTEDELVDYVTGRVASYKKPRRVVFVESVPKTAVGKLNRKTVRDMLA
                     QSP"
     gene            complement(7405..7719)
                     /locus_tag="VF226_03780"
     CDS             complement(7405..7719)
                     /locus_tag="VF226_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX7073137.1"
                     /translation="MAKKGKSQRRTVFSRYDSADYLKSEEDMIAYLDACMEEASDDPA
                     LVAVALGNVARAYGMARLAKETGLTREGLYKALSPEGNPTFGTVLKVVKALGLKLRPE
                     AA"
     gene            complement(7832..8284)
                     /locus_tag="VF226_03785"
     CDS             complement(7832..8284)
                     /locus_tag="VF226_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073138.1"
                     /translation="MRTARQASGHSIRRAQAPKSDETGLDRSSLWEAAAWQVREDRAF
                     YVPDVMSDRSYEADAELHRAANEHARDLRRAQRRLEDMLSLAGVLRASIGDEGDSRAM
                     QAETVLKIIERKADKALDQLDRHDGQHRNLFFAYFDLLDTAEEGGDGE"
BASE COUNT         1587 a         2810 c         2714 g         1513 t
ORIGIN      
        1 cacagcgctc acgaccggct cgcgttccat cggcacgaga gcgcctatat tgcgctgccg
       61 ctggaaggca gctacgagga gagcagcgcg gacggccgtt tcgcctgcgc gccgggcatc
      121 gccgtcgtgc acggggaatg gcacatgcac gccgatcgct tcaccggcgc gggcggccgc
      181 gtcctcaaca tcgatctccc ctccggcgct gtgccgcccg gcggttccgc ggccgtgacg
      241 gtggacttcg tggagatcga gaaactcgcc cggcggcagc cggcccgcgc cgcggccgcc
      301 gtcctcgaaa cctgggcgag cgccggagac ccggccggcg ccggatcggg cttagtctat
      361 gcgttcctgc gaagcgttcg gctcagcccg gcggcccgga ttgcggagct ggccgcggag
      421 ctgggtgtca gccgggaaca cctggcgcgc cgcgtcaagg cgcacctggg aatcggcccg
      481 agccgcctga ggagtgaatg gcgattccgg tgtgccgccg agctcctggc gcgtggcgaa
      541 tcgctcgcgc gggtcgcggt cgcggcgggc tactcggacc agagtcacat gacccgcgac
      601 atcaaggcgc ggctgggcag aacgccgggc cggctcggac gtctcagccg gccgcgacgc
      661 taagaagcat gaccagcccc acctcgatgg cattgccggt gccgtgcgcg atgatcggcg
      721 cgagcaggct ccgcgagcgc tccatcagcc agacgtaaac gagtccccag gcgaatgcgt
      781 agctttgctg cgcgacggcc atgtgcagcg gttcgaccac gaaggtccgc cagtgcgcga
      841 cgcagaacag caaggcgacg acgacgccgg cgagcgaaac gtcgaacctc cccgcgcgca
      901 cgcgcccggt cacgaacagg acgaggccgc cgaccagcag gccgcggaaa atggtttctt
      961 ccgccagccc ggtgatggcc atcgacacca gccagcccgt ggcatcgacc gggttcgtgg
     1021 cataaagcgt gtcgaacggc tcgcccagga gcaactgcgg ccagtagtcg gcgacgagca
     1081 tcacgagggc catgccgata ccgatgacga agccgagccc cgccagcgtg cggccgggcg
     1141 gccagcgcag gtgcgcatcg gctctcggca ggaccgtttt catcaccccg atgccgatca
     1201 ggccgagaat ggcctggaag agaatggcga ggccgacgta gatccaggcg cggtcgtccc
     1261 agctcggggg cccgttctcg tagagccacc tcgccggcac gcggccgagc cgcagcatcc
     1321 cttccatgag caggccggca acgacgatgg ccacgatggc gggaacgagc cgggaccggg
     1381 tacccgagat ctcgaagaaa cggcgcgtgg cgacggcttc gtgcatggcg gcaggatgcc
     1441 gccggttccg cggacggtat tgaagaaatg tgacgatcta tcggctttcc aggtcgaaag
     1501 caacgcggaa tcggaattcg aggtcccgag ctcggccgag tggtgtcggg cacaagcggc
     1561 aatcaacaga tccgtggcaa gtaacctaag cacagcggag gtgccggcca cgctccttcg
     1621 cgtggttttc gccccggcac gccgaccacg cccgatttcc gggattgcct tggcgtaatc
     1681 cccttgacgg gtgactggcg cgcattccag tgaccacaat ggtcaaggag cgttcagaac
     1741 gaaccgctaa taaacagata cggtccggta tgtctgacct gcgccttgcc gcgccagctt
     1801 tcgctcgtgg aagatatcgt catgttgatg tcccgatatc cgagcccgag gccgaagtgc
     1861 ctccacggct gatacacaaa atccgttgcg ctgctaaaaa tactgccatc cacctcgccc
     1921 gtatccagtg acagccgatc gacgcgtaca cgcagcagcc agcggttagt caaggcgaat
     1981 tgcgcgtaca tcgtgagcac tggcagaggc gccgactggc tcgcgcgctc ggatcccaaa
     2041 ttgcgtgtgg atagctccgc atcgaaactc atgacatgtg cgcccaggcc gaagccgact
     2101 tctttgtcct tcctgcggaa gaacgagtaa cccacactga tgcgcaggtc ttccaggtcg
     2161 gagctgccac taacgagggc gttcacgggg atgttcagat ctccccagtc gaccgtgcgg
     2221 gagagttctc tctcaccgct gcgatccagg cgaaagtact cggcctccag cagccaacgc
     2281 tcggaaaggc gcatgcgaaa gtcgaacatg ccgagcagat tcgacttctc cagcccaaca
     2341 tcatcttcga agtcaatgaa gctgccgagg ccgatcctgc ccctgttggc attggccgta
     2401 acgttggatt gaacccaagc ggcgccggcc cctacaaaga aacggtcgct catcatggga
     2461 tgctcgggca ccacgttgtc accagcgttc gcgtcgagcg ccagcagcag accgccgagc
     2521 gcggtgacgc tcagcgcggc aagtcgcctg aaaactcggc tcttcacacg cggagtctgc
     2581 gatgggatca gaatttactc tattggacgt tcgtctacct gatcgagaaa ttcgccgtag
     2641 ccctcgcgct ccatgtcgtc gcgatggaag aaccgaagcg acgcgaaatt gatgcagtag
     2701 cgcaggccac cgcgatcggc tggtagagcc acctcgccgg aactcggccc agccgcagca
     2761 tcccttccat gagcaggccc gcaacgaccg tagccacgat ggcgggaacg agccggggcc
     2821 gggtacccga gatctcgaag aagcggcgcg tggcgacggc ttcgtgcatg gcggcagaat
     2881 gccgcccgtt tggggaacgg tattgaaaaa tgtgaggtgg ccttgggcgc ggccctcggc
     2941 gtcccccgcc ggagccggga atacaaccgg gtgccggtcc gtccaagaca agagggtcac
     3001 tggcgtcatc gcgcgatcgg ctcggcactg atctcgttcg gcgccccgag cacggctcct
     3061 tggccggtga tcagcagaac gtccgcgatc gagccatgag cgatggaggt accatgaaac
     3121 gcaacattct gttggccacg gtcgtagcgg tcatgctcac ccatgccgcc tcagctcaag
     3181 atcggctgat gcagtccgcg caaggcctgt tcgagccgct cccgaccgag ccgcccgaca
     3241 tcgggatccg cagcacgccc gaacgcattg cgctcgggaa gatgctctat tttgacccgc
     3301 gcctgtcgga gagccacagc atctcgtgca actcctgtca cctcatcgga ctcggcggtg
     3361 tcgatctgca agaaacttcg atcggtcatc gctggcagca tggcgcgcgc aatgccccta
     3421 ccgtcttgaa cgccgttttc aacacggcgc agttctggga cgggcgcgcg ggtacgctcg
     3481 acgagcaggc aggcgggccg ctggtaaacc cgatcgagat ggggactacg gaggagcacg
     3541 tcgtactcca gctcgagggc attcccggtt acgttgacgc gttcgcgaag gccttcgcgg
     3601 aggcggacca gcccgtgact ttcgagaaca tacgcgacag catcgcgctg ttcgaggcga
     3661 ccctgatcac ccccaacgcg ccgttcgacc gatacttgcg aggcgacgcg caggccatga
     3721 cggatgacca gaagaccggc ctccagctgt tcatcgagaa gggatgcacc ggctgtcatc
     3781 acggcatgaa cgtcggaggc aacatgtatg ctcctttcgg cgtcgtcgag cggcccggcg
     3841 cagccctgtt accgccgacc gacaaggggc ggttcgcggt gacgaaaacg gtcagcgacg
     3901 aatacgtatt caaggttccg tcccttcgca acatcgcgtt gacgccgccg tattttcaca
     3961 gcgggcgggt ctgggatctc cgccaggctg tggccgtcat gggcacgagc cagctcggaa
     4021 ccccgctctc tgacgacgag gtcgtgcaga tcacggcgtt cctgcatgcc ttgaccgggg
     4081 atcagcccgt gatgacgctc ccgctcctcc cgccgagcgt cgccgcgacg cctcgaccga
     4141 atccttagcc gacaccctcg tcggtgctgt tgccggtgca gcgaaggctt tcactggcag
     4201 ccagcgattc actcgagccg tcccgcgcca tcgtttccag agcgagctcg ccggcactcg
     4261 caattctacg tatggcggca gcaggcccga ttcctgagac tgtgcttcgc gcagcaggcg
     4321 gaagccctgg ggggttcccg cgtgcgcgtt ccggcgacgc agagcggagg caggcccatg
     4381 gaggataccc acggctcgat tgaagctctt cccctcccgg gcgctgccgc gtcaatgcgg
     4441 atgtggcaac tcgatgacag ccggccggcc gacgaagcag cgttccgcaa agagctgctg
     4501 aagagcctgc cgaagctgcg ccgcttcgcg gtcaaccatt gccggagcaa agccgacacg
     4561 gacgacctcg tgcagctcac atgcgagcga gcgttggtcc attggcgaca atggagcgga
     4621 tgcggcagcc tcgatcgatg gctgatcagc atcctcgtca acgcctgccg cgacgaagct
     4681 cgagctcgcg caggaaaggc gctctacgcc ctggacgcca tcccggagat cgcggccggt
     4741 tcgagcacgg tggacagcgc ctaccttcag cagatacagg gagaagtgct gcgtatcccg
     4801 gacctttttc gcgacgtgct ttggctcgtc ggctgcgagg gtctgtccta tcgggaaacg
     4861 gcggagatcc tgcacgtgcc cattggtacg gtgatgagcc gtctgaaccg cgcacggcat
     4921 ctcctgcagg cgaggctcgc tggccgcggc caagccgctg ccgcagggcc accgcattag
     4981 gtcatcgaac gcgccggttc gggcagtgca cgatttccgt ttcattgcgg cctcggatgc
     5041 tatcaaaccg aagcgcgatc gcccgtcccg agctatagca caccgagggg ataggggcag
     5101 cttccacgct accatgtttg aaccacggga acttgtagta tggcaggaag atctcctcgc
     5161 cctgcggcgg cgccgtgcgg cgaaatgtca gttgcctcaa tgcccgaagt gctttttaat
     5221 gcggctagcg tctgctccgt ccaaagaagt tgacttctca accagcgcga caggtgggct
     5281 gacgaatgga cctacgccac ccgtcgcttc actttcactt ctacgccacg gccggtcctt
     5341 gccaacagtt ttacaagcat gtcgatgctg aagcgaccaa tcttcccccg cagtagatcg
     5401 ctgactcgtg actgctggat gccgagcaat tccgccgcct cgctctgcgt cacgccggaa
     5461 tcccggatca ccttctcgag ctgtcgcatc agatcggcgc gcagccgaag attttccgct
     5521 tcgccctttg cgaagccgag atcctcgaaa atcgaccaag tacactattc gcgtgtattc
     5581 ttatttcctc atgaagaacg tcacgatcac gcccgacgag aagacagcgg cctgggccct
     5641 gcaacatgcc gcgcgcagga gcatgagcct ctcacgtttc gtcggcgagc tctggagaag
     5701 accatgcagg agtctcgaga ctatgagcgc actatgcgcg agtacctctc gcgcaaaccc
     5761 gcgcccctga agcgccctcg cgcccgctat ccttcccgag acaagctcca tggccgcgac
     5821 cgtcttcgtt gacaccgata ttttcgttta cagccgcgac gccagcgagc cgaaaaagca
     5881 atcagtcgct gcgcgctggc tcgaggagtt gtggatcgag caggcgtgcc gggctggatc
     5941 gtactgattc accaactcgc gttcgcttaa aaaatcgagg tctgccgtgt ccaacaacgt
     6001 caccgtcgaa atccacgcaa cgcgcgcctc tcgcttgccg agcacttgaa gatgctggag
     6061 gagaccggct gcgaactgct ggtattcgga ccggatctgg ccgagtgggc ggcggcactg
     6121 ggtgcggaaa ttccggcgct cgcctgccac ggcctgggtg cgcgcctcga cgatggttgt
     6181 gacctgcttg ccgacgcgga aaacgcaagc gataccgcgc cggccatcga cgtgtccggc
     6241 gacgacgtca tcctcacgcg tttacttcgg gcactaccgg cacactgaag gcggcacagc
     6301 acactcaagg ctcctacgcc gccatctgca ggaacgtgct gatgaacctg ctgccggtcg
     6361 agcacgacga cgccatgctg cacgccgcgt cgctcatcca tgccagcggt gtgttcgtgc
     6421 tgccgttctg gctgcgcggt gcgcgaaccg tgatcatgcc gggctttgaa cccggcctgt
     6481 tcctggagct catcgcacgc gagcgcatca cggccgtaaa cgtggtgccc accatgctgc
     6541 agatgctgct cgaccaccct gctttcgcaa ctattgacgt ctcatcgctg cgccaggtca
     6601 tctacggcgc ctcgccgatg ccgcgaccgg tcatcgaacg cgcgatagac ctttggggcc
     6661 gcgaacgctt ctggcagtac tacggccaga ccgaatgccc cctcgcgatc gccgtgctgc
     6721 ggccggaaga tcacgaagag ggactgctcg gcgcctgcgg caagccggcg atcgacgtcg
     6781 aggtgcggct cgtcgacgag tccggcgccg acgtcgccgc cggcgagccg ggcgagattg
     6841 cggtgcgcgg gccttcgacg atggccggct attacaacgc gccggcgctc aaccgcgaaa
     6901 tgttcctgga cggcgggtgg ctgcgcacgc gcgacgtcgg gcagttcgac gagcgcggct
     6961 tcctgcactt gaaggatcgc acgtccgaca tgatcgtcac tggtggctac aacgtgtatc
     7021 cgcgcgagat cgaagacgtg ctggcgacgc atccggcggt agccgaatgc gtggtggtgg
     7081 gtgtggcgga cacgaagtgg gtggaggccg tgaccgccgt cgtcgcgctc aggccgcagg
     7141 cgcaggttac agaggatgag ctcgtggatt acgtgaccgg gagggtcgcg tcgtacaaga
     7201 agccgcggcg agtggtattc gtcgagtccg tgcccaagac cgccgtgggt aagctcaacc
     7261 ggaagaccgt gcgtgacatg ctggcgcagt cgccttaaag ggtgaatgcg gaaaaaactg
     7321 cctgtccatc actcgatgtc cttcatcaat tcccgcagca gtttgacctg ttgccgagtc
     7381 agatcctcgc gctcgctcag cctctcacgc agcctccggt cgtagcttca agccaagtgc
     7441 tttgacgacc ttgaggacgg tgccaaaggt gggatttccc tcgggcgaga gcgccttgta
     7501 aagaccctcg cgcgtgagcc cggtttcctt cgccagccgc gccatcccgt aggcacgcgc
     7561 cacgttgccc aacgcgacag cgaccaaggc aggatcgtcg gatgcttcct ccatgcacgc
     7621 gtccagataa gcgatcatgt cttcttcgga tttaaggtag tccgcactgt catagcgcga
     7681 gaagaccgtc ctccgctgcg atttgccctt cttggccatg tctcactctt cctgccaggt
     7741 ccttgcgatc tctttggccc gtttgatctc ggacgcaagg cacgttgtcg tggcgggcat
     7801 tcgggtaaca ctcgaatgcc cgccgtccgt ctcactcgcc gtcaccgccc tcttccgccg
     7861 tatccagcag atcgaaatac gcgaagaaca ggttcctgtg ctggccgtcg tgccggtcga
     7921 gctggtcgag cgccttgtcg gccttccgct cgatgatctt cagcacggtc tcggcctgca
     7981 tcgcccggct gtcgccctcg tcgccgatcg acgcccgcag cacgcctgcg aggctcagca
     8041 tgtcttcgag ccggcgctgg gcacggcgga ggtcccgggc atgctcgttt gcggcgcgat
     8101 ggagctccgc gtcggcctcg taggacctgt cggacatgac gtcgggcacg tagaacgccc
     8161 gatcttcccg gacctgccat gcggccgcct cccaaagtga cgaccggtca aggccggtct
     8221 catcggattt cggcgcctgc gcgcggcgga tcgagtgccc cgaggcctga cgcgcggtac
     8281 gcactgcact ggcgggactc gcgatcctgc ggccgggtac gtcgtaagtg gcggaaattg
     8341 gcttcgctca tggtgctgct cctctgccga tcgtgagcgg gagccagcgc aggcgctacg
     8401 aaaaagcgcg aggcccggaa cggttccggt cgggcgatcg cgcttcggtt tgatagcatg
     8461 ttgtcagcca tcgtcagcac ctgaactgct ggcatggtca gggccgttga ggggttcccg
     8521 cccctcttcg gtccgctttt tatagcataa gtgcgcgaca aaatacacga ggttcgtgtt
     8581 attatcgcgc gcatgaagaa cgtcacgatc accctggacg agaa
//
LOCUS       DASVDU010000055        10524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1084492, whole genome shotgun sequence.
ACCESSION   DASVDU010000055 DASVDU010000000
VERSION     DASVDU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084492"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF226_03790"
     CDS             complement(<1..428)
                     /locus_tag="VF226_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073139.1"
                     /translation="MGKSRNGQQRISRRGVIKGSLMAAAVGGTASFFGPWQHNRVWAQ
                     GQKKPIKLGLTCDASGQYGNSGQDDVRGIRMAIDEFNAKGGVLGRKIEWITADTETNP
                     ATGTRVAERFISQEECGFLLGALHSGVANAITQVANKYGT"
     gene            complement(864..2249)
                     /locus_tag="VF226_03795"
     CDS             complement(864..2249)
                     /locus_tag="VF226_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073140.1"
                     /translation="MTKNGLSLTLIVALGLILVWLIISHSFAAYFARSAPDLALSLNP
                     SQPRALAKLVTSRLIQQSDIVAGTEEKGAEAIGPGRLSDFAADRPLGRADEDGGDDAD
                     DGAGLADAAVRKEIRSLAERALAADPLNSRALYALGELAAAEHDEASASAFMRAAIRR
                     SFRQTGATYFMLMRSFSDRRYAEALDHADAILRTSPQLARHVVPLLARMAEEEDARGL
                     LEARLATDPPWRHAFLSSLPNAATDARTPFNILLALRDTPNPPSAADLRSYLEFLIRK
                     GFYELAYYVWLQFLTPDDLSSTGLLFNGSFERPISGLPFDWIIRHGPGVTIQISPAAG
                     LERGRALQIEFGHGRVEFGSVNQTTLLAPGRYRFTGSLKGELAGRRGLVWRMSCAGQR
                     NRILGASEMAMGLAPTWRPFSFDFEVPEDQSCRAQIARLTLDARSASERFVSGTIWYG
                     NLKIERILGPS"
     gene            complement(2309..2935)
                     /locus_tag="VF226_03800"
     CDS             complement(2309..2935)
                     /locus_tag="VF226_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX7073141.1"
                     /translation="MCGEKALRIALDLRHVPSLVRHVRGAPLPEGVPLLLRIAAGDGE
                     AEQHALQLVDRSLGEIREAAAFFIEQILFAPGSDSYRVLGARPDASAAELRRNMALLI
                     RWLHPDADTGERAVFVGRVTSAWDDLKTADRRAGYDKRMALTGDNPSSRHEAIRPSKK
                     RTAKRSRKSSLQHMHPRVKSRSLTVYRQRRSGLLARVLSLLAGRRWPT"
     gene            complement(2935..5229)
                     /locus_tag="VF226_03805"
     CDS             complement(2935..5229)
                     /locus_tag="VF226_03805"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX7073142.1"
                     /translation="MMADSSQEPGENRAARLVPAESAGPVVRDGYGGYYGGYGAGLDE
                     QDSFGQDLLEYWRIFYKRRWLILSIVVTSVVLALLVTMMMTPLYTATARIQIDREPPK
                     VLEGGNVVPTEGGSNFEYLKTEYELLQSRAIAERVTSALNLAADEDFLKPRSFSLIGA
                     IKSLFQPPSDEGPDKAALKRAATDVVLANLTVRPVPGSRLVDLSYTDPVPERAQRIAA
                     AYAEAYIAANLDKRFQANAYAKTFLEDQIAQLKLRLEESERKMLEFAEREQIVIVTEK
                     ASIAENNLASANAALGQLISERIKNEKLWKQVADADSMNVPQVLTNSVVDGLRARRNE
                     LVAEYQQKLEIFKPTFPAMLEISSKIKEIDRQLANEVQAIKASLKAAYETSLSQEQAM
                     RQQVEKLRAEVLDLQKRSIQYNILKREVDTNRALYNGLLQRFKEVDIAGGVGANNVFV
                     VDRAQVPKRPSSPRKGRALLLALALGLGIGLGTAYLLEQLDDTIRTPEEIERLAGLAT
                     LGVIPKVANDQALVADFADPRSAISEAYRSLCTALQFATDSGLPKSILITSATPSEGK
                     TITALAIARHFATMGQKVLLVDADLRMPSLHVRLGLDGSMGLSDYLTGACTPPEVMQR
                     SDITNLTFMASGPLPPNAADLLGSPRVLSLLSIGLEVFDLIVLDGPPVMGLADAQLLS
                     SSAAATVFVVGAGQARTGVIRGALKRLQLARGVLVGAVLTKFDAKQAGYGYGYGYGYG
                     GYDYTYGRNDLEAPGDRAKLAKSA"
     gene            complement(5322..6305)
                     /locus_tag="VF226_03810"
     CDS             complement(5322..6305)
                     /locus_tag="VF226_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7073143.1"
                     /translation="MSNQGETGRGSRSLPTLLELMLCAALLAGCGASLQNELATGVGS
                     SQVGSIATVNGAAADSGAPVATASTGDTAVVAKRGDTEHAGRPPYQSPAGAPVASSSV
                     PVVQKAALSGQRDALVEAPATAVARLPPAAANLTAVAEPGHVAYRIGPLDVLTVTVFR
                     VPELSAEVQVADTGTINLPLIGDVQAAGRTAKDVEKEVTAKLGEKYLQNPQVTVFIKE
                     YNAQRVTVNGAVKKPGVFPLKGRTTLLQLISMAEGLDHNRASSNVVVFRNSNGKRTAA
                     RFDLDQIHDGTAEDPTLQPGDIVVVDDSSAKILWQNLLRALPITNVFTAVL"
     gene            complement(6196..7083)
                     /locus_tag="VF226_03815"
     CDS             complement(6196..7083)
                     /locus_tag="VF226_03815"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX7073144.1"
                     /translation="MIDLHCHILPGIDDGAADVDAALDMARAFVADGVSVVACTPHIL
                     PGLYQNTVSGIRTAVAELQRRLDEADIPLKLVPGADNHIAPDCVAQLRDRRMLPLADS
                     RYVLIEPPHHVAPPRMDEVFFDLQVAGYVPILTHPERLRWIESHYALVATLARAGVWM
                     QITAGSLAGTFGSRAQYWAERMLDEGIVHILATDAHDVRHRRPDLSRGAELAARRVGD
                     SEAWHLVSTRPKGVLDNVPSSDLPLPGGGECSSTVAKGGGGGVEPGRDRARQQEPADA
                     SRAHAVRRFAGRLRRFLAE"
     gene            7321..7866
                     /locus_tag="VF226_03820"
     CDS             7321..7866
                     /locus_tag="VF226_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073145.1"
                     /translation="MYGLLAIGSQVALLLVSAHACCLVEFLFGFLQGSKAAVDREPMM
                     ISGWTKRAGIFSIALMLAFPAQSAVLEALQGGVLVSRGGSAYQLVDQPTQLRAGDSVL
                     ANPGGSARVVFENGCAVDIQPGMVFTVGEAPPCDSVGGETTGSTDYTTVAVGAAVVAG
                     GVGLALALGGGGGDGGGGASP"
     gene            8233..9765
                     /locus_tag="VF226_03825"
     CDS             8233..9765
                     /locus_tag="VF226_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7073146.1"
                     /translation="MLASVCGATLIASLILGGGTHSGFLSDAILQLIATPLLLLALWQ
                     LTRSPAATRLRGALAFCAAVVLVPVLQLMPLPPALWTRLPGHEVIAEAFTLSGQELPW
                     WPISVAPQETWLGLMSLVVPLAIFLGTVQLRRRERRLISLAVLTVGILSVFLGLLQVA
                     QGPKSALRFFTFTNPTEAVGFFANRNHFAALLYSLMLLAAAWTVHIGNQVTSYARKGH
                     DTPLILAVFAGFAVVVSLIAAQMMARSRAGLVLTILGLLGIWALTVRDRHRAAGGFAP
                     ARLLAGAVGLALVLGIQFALYRVLDRFEAGPLADARIPFALTTIEAAKAYMPLGSGAG
                     TFVPVYSMFEKIEDARQFYANRAHNDVLEVWLEAGVFAVLLMGVVLAWLIRRSIALWR
                     ERPRPGGEIDLLLARAAGLVLLLLSAHSLVDYPLRTAAMMALTAFVSGLMFESPDKPK
                     PPRNAGSRRRSQRSTLILAQAPTPVSSAVPGQTRSQDWPNMEWPEAWRRAAKRDGEPS
                     SD"
     gene            10004..>10524
                     /locus_tag="VF226_03830"
     CDS             10004..>10524
                     /locus_tag="VF226_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7073147.1"
                     /translation="MHRVSLLLIDLFLVLLATILASVLRHDFQVSPDRLAALFPYLLL
                     TVSTAAIVLPAFGTGRSVWRLTTMRDYLHLAAATVAIVVCSVALGFAYNRLEGVPRSV
                     PVLQGLVMLFALVGVRVLLRLRHAARQRPRPLMPTPEHSLPQRTVLIVGLNRLAEAYL
                     LSISEFARGRVRVA"
BASE COUNT         1880 a         3317 c         3242 g         2085 t
ORIGIN      
        1 atggtgccat acttgttggc gacctgggtt atagcgttgg cgacacccga atgaagcgcg
       61 cccagcagga agccgcattc ctcctggcta atgaatctct cggcaacgcg cgttccggtc
      121 gctggattgg tttcggtatc ggcggtgatc cattcgatct tgcggccgag cacgccgccc
      181 ttggcattga attcgtcgat ggccattcgt atgccgcgga catcatcttg cccgctgttc
      241 ccgtactggc cgctggcatc gcaggtcagg cccagcttga tcggcttttt ctgtccttgc
      301 gcccagacgc ggttgtgctg ccagggacca aagaacgatg ccgtcccacc gactgccgca
      361 gccatgaggc tgcccttgat tacaccacgc cggctgatgc gctgctgccc gttccgggat
      421 ttgcccatcg tttcctccca agttcagttt ccagcacaat atagtccaaa accaagctgg
      481 gcaggcaagc tactattagg ccctatggca tacggctgga ttggattttt tcccagcggc
      541 gcctcagcgc cgagcagcgc ctcccgttcg gagggaaccc caaggcatcg tgagcgccag
      601 aaaggaggct gaccagccgg aggtggttct tcaggtccta aggcgtttgc ggaatgcccc
      661 tttgctcgaa ggtaagcggg tgaggcgcaa agggcgaggc ctaacggcca tctccctgaa
      721 aatgggctgg cgaggggcct tctacaactt ggccattgcc ggcgggcgat gcgatgcctg
      781 ccccggccgg gatgatgcat tcggggtctg tgtgcgcaca tcaacgtcag cgagctgcag
      841 ggcttgaact gccgaactgg tcctcagctc ggccccaata tccgttcgat cttgagattg
      901 ccgtaccaga tcgtgcccga gacgaagcgc tcggatgcgg agcgtgcatc gagggtcagc
      961 cgggcaattt gggcccggca agattgatcc tctggcactt cgaagtcgaa gctgaacggg
     1021 cgccaggtcg gcgccagccc cattgccatc tcgcttgctc cgagaattct attgcgctgc
     1081 ccggcacagg acatacgcca gacgaggcca cgacgcccgg cgagctcacc tttgagagag
     1141 ccggtgaagc ggtacctgcc cggagcaagt agcgtcgtct ggttgacgct gccgaactcg
     1201 acgcgcccat ggccgaactc gatctgcagc gcgcgcccac gctccagacc ggcggccgga
     1261 gaaatctgga tcgtcacccc ggggccgtgg cgaatgatcc agtcgaaagg cagcccggat
     1321 atgggccgct cgaatgagcc gttgaacaac agcccggtgc tgctcaggtc gtccggcgtc
     1381 aggaactgca accagacata ataggcaagc tcgtagaaac ccttgcggat gaggaattcc
     1441 aggtagctcc tcagatcggc ggcgctgggc ggattgggcg tgtcgcgtag cgccagaagg
     1501 atattgaacg gtgtacgcgc gtctgttgcg gcgttcggca gcgacgagag aaatgcatgc
     1561 cgccagggtg gatcggtggc caaccgggct tcgaggagac cccgcgcgtc ttcctcttcg
     1621 gccatgcgcg cgagcagcgg aacgacatgg cgcgccagct gcggagacgt cctcaatatc
     1681 gcatcggcat gatccagagc ctctgcatag cgcctatccg agaagctgcg catcagcatg
     1741 aaatacgtcg ccccggtctg gcgaaaagac cggcggatcg cggcgcgcat gaacgctgat
     1801 gcgctcgctt cgtcgtgctc cgccgctgca agttcgccga gggcgtaaag agcgcgggaa
     1861 ttgagcggat cggcagcaag cgcacgctcc gccagcgatc gaatttcttt ccgcaccgct
     1921 gcatcagcga gcccagcgcc atcatcagca tcatcgccgc cgtcttcatc ggcgcgaccc
     1981 aatggtctgt ccgcagcgaa atcagagagc cggcccggtc cgatggcttc ggcgcccttt
     2041 tcctcagtgc ccgcgacaat atcggattgc tggatcaatc tggatgtcac cagcttcgcg
     2101 agcgcccttg gttggctggg gttgagcgac aacgccagat caggcgcgga ccgggcgaaa
     2161 taggcagcga agctgtgcga tatgatgagc caaaccaaaa tcagtccgag cgcgacgatc
     2221 aacgtcaggc tcaaaccatt ctttgtcaat ttgttccaac gcggtagctc ggcgttttcg
     2281 ctcatcgctc ggagcccatg tcaacgggtc acgtgggcca tcgtcttccc gccaatagcg
     2341 atagaacacg cgccagcaat ccgctccgtc gctggcgata aaccgtaagc gaccgcgact
     2401 ttacgcgcgg atgcatgtgc tggagcgagc tcttccggct gcgttttgcc gtccgctttt
     2461 tggacggcct gatcgcctca tgtctgcttg acgggttgtc tccagtcaga gccatccttt
     2521 tgtcgtaacc ggcgcgccga tctgctgttt tcagatcgtc ccaggcgctg gtcacgcgac
     2581 caacaaacac ggcccgttcc cccgtatcgg catcgggatg cagccacctg atgagaagag
     2641 ccatgttccg ccgaagctct gcggcggaag cgtccggcct ggcgccgagc acgcgatagc
     2701 tgtcggaacc gggtgcgaac aagatctgct cgatgaaaaa agccgccgct tcgcggatct
     2761 cgccaagcga gcgatcgacg agttgcaagg catgctgctc agcttcgccg tcgccggccg
     2821 cgatccgaag caagagcggt acgccctccg gaagcggcgc gccgcgaaca tggcgcacga
     2881 gagatggtac gtgccgcaga tcgagtgcga tgcgtagcgc cttctcaccg cacatcaggc
     2941 actcttggcc agcttggcac gatcgcccgg ggcttcgagg tcatttcgtc cgtacgtgta
     3001 gtcgtacccc ccatagccgt aaccatagcc gtatccgtag ccggcctgct tggcatcgaa
     3061 cttggtcagc actgcgccga cgagcacccc gcgcgcgagc tgcagccgct taagcgcacc
     3121 gcggatcacg cccgtgcgcg cctggcctgc cccgacgacg aacacggtcg ccgcagcgct
     3181 gctcgaaaga agctgcgcat ccgccaaccc catcaccggc gggccgtcga ggacgatcaa
     3241 atcgaagact tcgagcccga tcgacagaag ggacagcacg cgcgggcttc cgagcagatc
     3301 ggccgcattc ggcggcagcg gaccggaggc catgaaggtc aaattggtaa tgtctgagcg
     3361 ctgcatcacc tcaggcgggg tacacgctcc tgtaaggtaa tcactcagcc ccatgctgcc
     3421 gtcgagaccg agcctgacat gtagcgacgg catacgcagg tccgcatcaa cgagcagaac
     3481 cttctgaccc attgtggcga aatggcgggc gatcgcaagc gctgtgatcg ttttcccctc
     3541 agacggcgtt gcactggtaa tgagtattga tttgggtaaa ccgctgtcgg ttgcaaattg
     3601 cagcgccgtg cacagtgatc tgtaagcttc ggagatggca gagcgcggat cggcaaaatc
     3661 ggctacaagg gcctggtcat tcgcaacctt gggaatgacg ccaagcgtcg ccagtccggc
     3721 gaggcgctcg atctcctcag gggtgcgaat cgtgtcatca agctgttcga gaaggtatgc
     3781 agtgccgaga ccaataccga gacccagagc cagtgccagc agcagcgcgc ggccttttcg
     3841 tggtgacgac gggcgcttgg gaacctgggc acgatcgacc acgaacacat tgttggcgcc
     3901 cacaccgcca gcgatatcga cctccttgaa gcgctgcagc agcccgttat aaagggcgcg
     3961 gttcgtatcc acctcgcgtt tcaggatatt gtattggatg ctgcgcttct gcagatccag
     4021 cacctcggca cgcagcttct ccacctgctg gcgcatggcc tgttcctggc tgagcgaggt
     4081 ttcgtaagct gctttcagcg aagccttgat cgcttgcact tcgtttgcca gttgccgatc
     4141 gatttccttg atcttggagc tgatctccag cattgccggg aaggtcggct tgaagatctc
     4201 gagcttctgc tggtactccg cgacgagctc gttccggcga gcgcgcagtc catcgacgac
     4261 gctgttcgtg agcacctgcg gcacgttcat gctgtccgca tcagccacct gtttccacag
     4321 tttctcgttc ttgatgcgct ccgaaatgag ctggccgagg gctgcgttgg cactggctag
     4381 gttgttttca gcgatcgagg ctttttctgt aacgataacg atctgctcac gctcggcaaa
     4441 ctcgagcatt ttgcgttcgg actcctcgag gcgcagcttc agctgcgcga tttgatcctc
     4501 gaggaatgtc ttggcgtagg cattcgcttg gaaacgcttg tccaggttcg ccgcgatata
     4561 ggcctccgca taggcagcgg cgatccgctg tgcgcgctcg ggcaccgggt ccgtgtagct
     4621 gaggtccaca agacgggagc cgggcacggg ccgcacggtg agattggcta ggaccacatc
     4681 agtagcagcg cgtttgaggg cagctttgtc tggcccctcg tcggatggcg gctggaacag
     4741 gctcttgatg gcaccgatga gtgagaagct gcgtggcttc agaaaatctt cgtccgctgc
     4801 gagattcagt gccgatgtca cccgttcggc gatggcgcgg ctttgcagca gctcgtattc
     4861 ggtcttgagg tattcgaaat tcgagccgcc ctcggtggga acgacgttgc cgccttcgag
     4921 caccttcggc ggctcccgat cgatttggat gcgagctgtc gccgtgtaga ggggcgtcat
     4981 catcatcgtg acgagcagcg cgagcacgac gctcgtcacg acgatcgaaa ggatcagcca
     5041 gcgacgcttg tagaatatgc gccagtattc cagcaggtct tgcccgaagc tgtcctgctc
     5101 atccaaaccg gcgccatagc cgccgtagta tccaccatag ccgtcacgca ccaccggccc
     5161 tgccgactcg gcggggacca accgggcggc acgattctcg ccaggctcct ggctcgagtc
     5221 agccatcatc agcactcaga agtctggaag agcctagcct ggaatcggcg cggcaaacgc
     5281 ccagcatgag gttcggctcc gatccatcag cataagacaa attatagaac agcggtgaag
     5341 acattggtta tcggcaaagc acgcaacaaa ttctgccata atatcttggc ggacgagtcg
     5401 tcgacgacca cgatgtcgcc gggctggagc gtcgggtcct ctgccgtacc atcgtggatc
     5461 tgatcgagat caaatcgcgc ggcagtccgt tttccgttac tgttgcggaa cacgacgacg
     5521 ttgctcgagg cccgattatg gtcgagccct tcggccatgc tgatgagctg gagcaaggtc
     5581 gtgcgtccct tgagcgggaa aacgccgggc ttcttgacgg ccccgttcac ggtcacacgc
     5641 tgcgcattat attccttgat gaaaacggtc acctgcgggt tctggagata cttctcgccg
     5701 agcttggctg tgacttcctt ttcaacgtcc ttggccgtgc gcccggccgc ctggacatct
     5761 ccgatcagcg gcagatttat cgttcccgtg tctgcgacct gcacctccgc agagagctcg
     5821 ggcacgcgaa acacggttac ggtcaacaca tcgagcggcc caatgcggta ggccacatgc
     5881 cccggctccg ccacggccgt gaggttcgca gcagcgggag gcagacgagc gaccgccgtc
     5941 gcgggcgcct cgaccagcgc atcacgttgt ccactgagcg ctgccttctg caccacaggc
     6001 acgctggagg acgcaaccgg cgcacccgca ggcgattgat aaggtggtcg acccgcgtgc
     6061 tccgtatccc cgcgcttcgc aacgaccgcg gtgtcgcccg tggatgcggt tgcgacaggc
     6121 gcgccgctgt ccgcggccgc tccattcacc gttgcaatgg aaccaacctg cgaagatccg
     6181 acgccggtcg ccagctcatt ctgcaaggaa gcgccgcagc cggccagcaa agcggcgcac
     6241 agcatgagct ctagaagcgt cggcaggctc ctgctgccgc gccctgtctc gccctggttc
     6301 gacacccccg cctccaccct tcgcaaccgt cgacgaacac tcaccgcctc ctggcagggg
     6361 taggtccgat gacggaacat tgtcaagcac cccttttggc cgtgtcgaga ccagatgcca
     6421 cgcttcgctg tcgccgactc gacgcgccgc aagctcggca ccgcggctga gatccggtcg
     6481 gcggtgccgc acatcgtgag catccgtagc cagtatatgg acaatgccct cgtcgagcat
     6541 ccgttcggcc cagtattgtg cacgggagcc gaacgtgccg gcgagcgacc cggccgtgat
     6601 ctgcatccac accccggcac gcgccagtgt cgcgacgagc gcatagtgtg actcgatcca
     6661 ccgcaaacgc tccgggtggg taaggatcgg cacgtagccc gcaacctgca gatcgaagaa
     6721 cacctcatcc atccgcggcg gcgcgacgtg gtgcggcggc tcgatgagga cgtagcgcga
     6781 gtcggccagc ggcagcatgc gtctgtcacg gagctgcgca acgcagtccg gtgcaatgtg
     6841 attgtctgcc ccaggaacga gcttcagcgg aatgtccgcc tcgtcgagtc ttcgctgcag
     6901 ctcggcgacg gcggtacgaa tcccgctaac cgtgttctga tagagaccgg gcaggatatg
     6961 cggcgtgcat gcgaccacgg agacgccgtc ggcgacgaac gcgcgcgcca tatccagtgc
     7021 tgcgtccaca tcggccgcgc cgtcgtcgat gcccgggagg atgtggcagt gcaggtcgat
     7081 catagacttg gccccgctct attgggcgcc cgcaagcttg gatcgtcaag acctgagcgc
     7141 acccgacctg ctggccggcg agcctcgcca gcgtcgccca gctttagcgc caagggcaaa
     7201 ggcaatgcaa cttaaaattg tgtcgactgc agatcgtccc ccaagcgcgg agaactgttg
     7261 cccgaaaaca tcatgtctcg cctgattgcg ctcgcaccgc cgtcgtaaac gattggtagt
     7321 gtgtacggac ttcttgcaat cggtagccaa gtggcgttac tgcttgtgtc ggcgcatgcg
     7381 tgttgtctag ttgagtttct ttttggtttt cttcagggct cgaaagctgc tgtcgatcgg
     7441 gagccgatga tgatttcagg gtggaccaaa cgggccggca ttttttccat cgccttaatg
     7501 cttgcctttc cggcgcaatc ggcggttctc gaagcactcc agggtggtgt gctggtcagc
     7561 cggggtggca gtgcctatca gctcgttgac cagccgacac aactccgggc aggggactcg
     7621 gtgctcgcca atcctggggg gagtgccagg gtcgttttcg agaacggttg tgccgtggat
     7681 atccagccgg gcatggtttt caccgtcggc gaggcgccgc cttgcgactc tgttggtggt
     7741 gaaactacgg ggtccactga ctacacgacc gtcgcggtcg gggccgcagt cgtggccggc
     7801 ggcgtcggcc ttgcgttggc cctcggcgga ggtggcggcg atggcggcgg cggcgcgagc
     7861 ccttgaagca ttccgagttc acgcgttaac gcgattgtaa gaaacatcgg aaatttccgc
     7921 gcgtagcctg cttcgagcca gctccccgct gaatgcaaag cattctgacg aggtaagctg
     7981 caggacccga tgggcaaaag ggcccatccg gagcgcgtct ctgcgagttg cgggctatgc
     8041 tcagaaagcc gcgcgcttct tcaatccaag gatctgccgt gcgttgggcg atgttgctcc
     8101 tgccgtataa ttcagtccta atgcgcccat agttcagcac cgcgtcgcaa ccagattgcc
     8161 cggcaggtct tgaccgcact cgacacatcg caacccgaca tcgccgcgcg accagaggtg
     8221 gaaagctcgg ccgtgcttgc atccgtctgc ggcgcgacac tcatcgccag tctgatactg
     8281 ggcggcggaa cacacagcgg ctttttgtcc gacgccatcc tgcagctgat cgcgacaccc
     8341 ctgttgctgc tggcgctctg gcaactgaca cgcagtcccg ccgctacgcg cttgcgtggg
     8401 gcgctcgcgt tctgcgccgc cgttgtgctc gtgcctgtcc tgcagctgat gccgctgccg
     8461 ccggcactgt ggacgagatt gccgggccat gaggtgatcg cggaagcctt tacgctgagc
     8521 ggccaggagc tgccatggtg gccgatcagc gtggcgccgc aggagacctg gctggggctc
     8581 atgtcgctcg tcgtcccgct cgccatattc ctcggcaccg tgcagctcag gcggcgcgag
     8641 cgccgtctca tcagcctcgc cgtgctgacc gtcggcattc taagcgtatt cctcgggctg
     8701 ctgcaggtgg cgcaggggcc gaaaagcgcc ctccggttct tcacattcac gaacccgacc
     8761 gaggccgtcg gcttcttcgc caaccgcaat cattttgcgg cactgctcta tagcctgatg
     8821 ctgctcgcgg cggcctggac ggtgcacatc ggcaaccaag tcaccagcta tgcgcgcaag
     8881 ggccacgata cgccgctcat cctggccgtg tttgccgggt tcgccgtggt ggtcagcctc
     8941 atcgctgcac agatgatggc ccgctcccgc gcgggcctgg tgctgacgat cctgggtctt
     9001 ttgggaatct gggcgctgac tgtccgtgac agacatcgcg cagcgggcgg cttcgcacct
     9061 gcgcggcttc tggccggcgc ggtcggtctt gcgctggtgc tcgggattca gttcgccctc
     9121 tatcgcgtgc tcgaccgttt cgaggcaggt ccgctcgcgg acgcgcgcat tccattcgcg
     9181 ctgaccacca tagaggcggc caaagcctat atgccgctcg gctcgggcgc cggcacgttc
     9241 gtccccgtct acagcatgtt tgagaaaatc gaggatgccc gccagttcta cgcaaaccgc
     9301 gcccataacg acgtgctgga ggtctggctc gaagcgggcg ttttcgccgt tctgctgatg
     9361 ggcgtagtct tggcatggct cattcggcgc agcatcgcgc tgtggcgcga acggccacgg
     9421 cccggcggtg agatcgatct gctgctcgcc cgcgccgcag gcctcgtcct tctgctgctc
     9481 tcggcgcatt cgctcgtcga ttatcctctg cggacagcgg cgatgatggc gctcaccgca
     9541 ttcgtttctg gactgatgtt tgagtcgccg gacaagccaa agccgccgcg gaatgctgga
     9601 tcgcggagac gatcgcagcg ttccacatta atcctcgcgc aggccccgac tccggtcagt
     9661 tcagccgtgc cgggtcagac gcgcagtcag gactggccga atatggagtg gccggaagcc
     9721 tggcgtcgcg cggcgaagcg ggacggcgag ccgtcctcgg attgactgcg atcaagcgaa
     9781 acacgtccag atcgcaactg atcaacttga agatgtgaaa accagcggct cttctgctcg
     9841 catcgcctcg tcgcaacagt gcaacagttg tgcacgacca cggaaaatct gggactcact
     9901 gcgcgagccc tcttgcaata tgattgtgaa ggcataacct tgcgccaagc ttgtctctgc
     9961 gttttttgtt gaatgcattt tcaccgggtt ccgcggggga aaaatgcacc gcgtttcatt
    10021 gctgctcatt gatttgttcc tcgtgctatt ggccacgatc ctggcatcgg tgctgcgcca
    10081 cgatttccag gtgtcgcctg accgtctggc tgccctcttt ccctaccttc tgcttacggt
    10141 ttcaacggca gccatagtgc tgcctgcctt cggcacgggt cggtccgtct ggcgactgac
    10201 gaccatgcgg gattacctcc accttgccgc cgcgacggtc gcgatcgtgg tctgcagcgt
    10261 cgccctgggt tttgcttaca atcgcctgga aggcgtcccg cgctcggtgc cggtgctgca
    10321 gggcctcgtc atgctgttcg cgctggttgg cgtgcgcgtg ctgctgcgcc tgcgccatgc
    10381 cgcccgccag cggccgcggc ctctgatgcc gacgccggaa cacagccttc cgcagcggac
    10441 ggtgctcatc gtcggcctca accgcctcgc cgaggcctat ctgctctcga tcagtgagtt
    10501 cgccaggggc cgcgtgcggg tggc
//
LOCUS       DASVDU010000056         7611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1277164, whole genome shotgun sequence.
ACCESSION   DASVDU010000056 DASVDU010000000
VERSION     DASVDU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277164"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VF226_03835"
     CDS             <1..748
                     /locus_tag="VF226_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX7073148.1"
                     /translation="RVLPFVFDLVNLVNDLPIADSSKKHSIKGDVGVSNDPDGRETIK
                     YLTNVRSMLERICSVHPSSLGLHPAVYFYSRRGVFQPQALLSFIAFFKAFKTDDYKQF
                     TTIRRKFEEFLIANRGVSEVVHTLGSGARSRPRMVSLYRKLQAEFSNGASIDEVERKL
                     AHDPDFSFLFGGDDSPSRAKGTKFKRDTKGAAFLRDALPNASRCQTCGGLLHRNGIQT
                     GHLIARREGGTGSLDNAILQHPFCNSTVAN"
     gene            873..1394
                     /locus_tag="VF226_03840"
     CDS             873..1394
                     /locus_tag="VF226_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073149.1"
                     /translation="MLTRTSPILVGKYELVTTTDRLLWWEDQIRRQGICAFDTETTGL
                     DPHTAELVGIALALPSGEAWYVPVGHQGVEQLPLPTVLNVLGPVLSMPLITKVMQNAK
                     YDLHVMARYGIEVADVEDTMLKSYSLFGGKHRHNMDDLARMYLKWFRSGSTSRQAMPK
                     TRKPSSPSRASVA"
     gene            complement(1514..1672)
                     /locus_tag="VF226_03845"
                     /pseudo
     CDS             complement(1514..1672)
                     /locus_tag="VF226_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024643.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1823..2311)
                     /locus_tag="VF226_03850"
     CDS             complement(1823..2311)
                     /locus_tag="VF226_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073150.1"
                     /translation="MALRRRSGARRLLLDRSSRLYVNEALLTGEPYPVEKRAHGSEDA
                     GAATPTNCVFMGSSVISGTARAVVVATGRATRIGAIAGALRKEPPPTAFSLGIRRFGM
                     LLLRLTVSLVLFVLLVNLLFGPTAPRVGSIRARAGSRAHARILADDPYRDACSRRGAH
                     GA"
     gene            complement(2230..2754)
                     /locus_tag="VF226_03855"
     CDS             complement(2230..2754)
                     /locus_tag="VF226_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX7073151.1"
                     /translation="MNKYADSGLGEVDPSRRALDEIYATLGTSANGLRAEQAAARHAK
                     YGPNRIEEPRPLQLIAGLAKPFSNPLVLILLFAAGVAGLTHEHASFLIIIAIVVLSVV
                     IDFVQEHRAESAAKALRRRVALRVTAVRSGVNREVAASDLVPGDVVLLCAGDLVPADC
                     CLIEARDSTSTRHC"
     gene            3084..5360
                     /locus_tag="VF226_03860"
     CDS             3084..5360
                     /locus_tag="VF226_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7073152.1"
                     /translation="MKRLLLPVTSALLVAGGIALLYGRAELSSSFWLVGTAVVLAVLL
                     VDVALTFSRGEYGLDLIAALAMAGTLVLGEHLAGIIVALMFTGGEALEGLAQRRARQE
                     MTALLSRLPRTAARSSDGQLEEVALEELKVGDRILVRRGEVVPVDGTVKSDAAVLDQS
                     ALTGEALPVRRAAGEPVMSGSTNAGDAFELQATSTASDSTYAGIIRLVEAAQAAKAPM
                     SRLADKYALVFLAITLAMAGGAWLLSGDPVRALAVLVVATPCPLILAVPVAIISGVSR
                     CAKRGVLVKGGGALELLARIKTVLLDKTGTITDGRARLIEMKARTDLDPLEVLRLAAS
                     LDQGSHHVIAQALVSAARERGLALETPTGVRELPGFGVSGRVGNHSLAVGGWEFVKST
                     VEADGFTDEIDTWIRRDGTVAVAAAIDGKLAGAFLLADQVRPEAGTVLRNLRDVGVDR
                     IVLVTGDRADLAASVAAFLSFDHVASELQPQDKTRAVYEEKSRGPVMMVGDGINDAPA
                     LAAADLGVAMGARGAAASSEAADVVILVDRLDRLVDAVRIARRSRGIALQSVLAGMGL
                     SGAGMFAAAFGYLSPVQGALLQEGIDLLVILNALRALGVPLWVRLKPRTLSSEELQAL
                     EAEHRSLAEVVDSIRYTADRIRHLSGPDVARELAALETVLRERLLPHERREDEQVYSR
                     IRRSAGDPNALAGMSRTHMEIQRQVHALTSLRKALPVAGPNSTQRYEIERLLHGLEAI
                     TRLHFAQEDEIYRSLEFD"
     gene            complement(5551..6945)
                     /gene="lpdA"
                     /locus_tag="VF226_03865"
     CDS             complement(5551..6945)
                     /gene="lpdA"
                     /locus_tag="VF226_03865"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7073153.1"
                     /translation="MEPISCTVLVLGGGPAGYVCAIRAGQLGLDVVLVEARHLGGTCL
                     NIGCIPSKALIHAADQFYLARRFAAGTAPGVRVAEPTFDLPETMAWKDGIVSKLRGGV
                     FGLLRRAKVKVVTGTGVLRDGKTCLVETETGDQMITAEHVVIATGSEPVSLPNLPFGG
                     PVVSSAEALSFDTLPQRLVVVGGGYIGLELGTAFAKLGSVVTVVEATERILPQYDAEL
                     TRPVLSRLEKLGVRVLLKAKALGLDSAGTALKVMPDGGEEERISADKILMTVGRRPRL
                     EGYGLAELDLEMNGTAIWIDDQCRTSMRNVWAIGDVTGEPMLEHRAVAQGEMVAEIIA
                     GHKRSFDKVAIPAVCFTDPEIASAGLSPDEARSAGLPIKVGTFPFAANGRAATLGSED
                     GFVRIVARTDNHVILGIQAVGQGVSELSGSFALALEMGARLEDVAGTIHVHPTLSECL
                     REAAAAALGQPLHA"
     gene            complement(6946..>7611)
                     /locus_tag="VF226_03870"
     CDS             complement(6946..>7611)
                     /locus_tag="VF226_03870"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7073154.1"
                     /translation="RKIAQRMQDTKRRVPHFSYIEEVDVTDLEALRADVNANRKPGQP
                     KLTVLPFLMRALVVALRDYPQMNARYDDDTDILHRYGGVHIGIAIQTPNGLMVATVRH
                     AEARTIWDCASEVQRLSEAARSGTISREELAGSTITITSLGPLGGLASTPVINAPEVA
                     IIGVNKIAIRPVWRGTSFEPRKVMNLSSSFDHRIIDGWDAAQFIQRLRSLLESPAKIF
                     MET"
BASE COUNT         1404 a         2390 c         2411 g         1406 t
ORIGIN      
        1 ccgcgtcttg ccgtttgtgt tcgacctcgt caatttggtg aacgatcttc caattgctga
       61 ctcgagcaag aagcacagta tcaaaggtga tgttggagtg agtaatgacc cagacggccg
      121 cgaaactatc aaatacttaa caaatgtccg ctcgatgctg gaacggatct gctcagtaca
      181 tccgtcgtcg ctcggtctgc atcctgccgt atatttctat agccgaagag gtgtgtttca
      241 accgcaagct ctgttgagtt tcattgcttt ttttaaagca ttcaaaacgg atgattataa
      301 acaattcacc accataagaa ggaagttcga ggagttcttg attgccaatc gaggcgtttc
      361 agaggtagtg catacccttg gaagcggggc cagaagtaga ccgcggatgg tgagtctcta
      421 tcggaagctc caagcggagt tcagtaatgg tgcctccatc gatgaagttg agagaaaatt
      481 agcccacgat ccagatttct cattcttgtt tggtggggac gacagtccca gccgtgcgaa
      541 aggcacaaag ttcaagcgcg atacgaaagg agccgccttc ctccgtgatg cgcttccaaa
      601 tgcaagcagg tgccaaactt gcggtgggct actccatcgt aacggcatac agactggcca
      661 cttgatagcc cgacgggagg gaggtacggg ctcgctggac aatgcgattt tgcagcatcc
      721 attttgtaat agcacagtgg cgaactaacc atggcagtct ggcatagggc gttgcgcttg
      781 tggacgcgcc tagcctgtcc gctaatctct tcgtggcacc cccgcaaatc atggcacgct
      841 gaccaaaaac acgcgtgaat tgtgggattg ctgtgctcac gagaacctcg cccatcctgg
      901 tcggcaagta cgagctggtc acgacgacag accgtctgtt gtggtgggag gaccagattc
      961 gccggcaggg gatctgcgct ttcgataccg agaccacggg gcttgacccg cacacggccg
     1021 agctggtcgg cattgcgctg gcactcccgt ccggcgaagc ctggtatgtg cctgttggcc
     1081 atcagggcgt cgagcagctc ccgctcccga ctgtgctgaa cgtgctcggg ccggtcctgt
     1141 ccatgccgct catcaccaag gtcatgcaga acgccaagta tgacctgcac gtgatggcgc
     1201 gctatggcat cgaagtggct gacgtcgaag acacgatgct caagtcgtac tccttgttcg
     1261 gcggcaagca ccgtcacaac atggacgatc tggcgcgcat gtacctcaag tggttccgct
     1321 ccggctcgac ctcacgccag gcgatgccca agacccggaa gccttcctcg cccagccgcg
     1381 ccagcgtggc gtgagccatc gcgatgcttt cgtcggtgag cggcctggcg ccatccttcc
     1441 ccgccgcctg ccaggtcgat gaatgcctga gcacgtcctc aggagcaccc ttaacgatca
     1501 gaaatcgccg cccggcgcgt tcggcaagca ccgatacgcg ccgacgctcg aaatcgaacg
     1561 gcacctcgtc gaccttgcgc cagtctgaca ggtcctccgg tgccgcctcg agaattgcct
     1621 cgtcgagggg gcttttgagc cggtttcgaa agccgcattg acatgggccc acgttagaat
     1681 gtcgcggcta tcatttccgg cgatatcaac ctcgcgaata agtcggatct gtgcttccgt
     1741 cagcgtgccg gtcttgtcgc tgcacagaat gtccatggcg ccaagatcat gcaccgcaga
     1801 aagccgtcgc acgatcacct gctcatgcgc catgcgcacc gcgccgcgag caagcgtcac
     1861 ggtaaggatc atcggcaaga attcgggcgt gagcccgact gccagcgcga gcgcgaatag
     1921 aaccgactcg aggagcggtc ggcccgaaca acaggttcac cagcagcacg aacaggacga
     1981 gcgacacggt gagccgcagg agcagcatgc caaatcggcg aatgccaagg ctgaatgcgg
     2041 tcggcggtgg ctccttgcgc agcgctccgg cgattgcccc gattctcgtt gctcgaccgg
     2101 tcgctacgac aacggcccgg gctgtgccgc tgatgaccga gctgcccatg aaaacgcagt
     2161 tggtgggcgt cgccgccccg gcatcttcgg agccatgggc acgtttttcg acaggataag
     2221 gctctccggt cagcagtgcc tcgttgacgt agagtcgcga gcttcgatca agcagcagtc
     2281 ggcgggcacc agatcgccgg cgcagagcaa tacgacatca ccgggcacga gatcggaagc
     2341 cgcaacctcc cggttgacgc cgctgcgcac cgccgttacc cgcagggcga cccgccggcg
     2401 cagcgccttg gccgcactct cggcccggtg ctcttgaacg aagtcgatca cgaccgacag
     2461 gacgacgatc gctatgatga tcaggaagct cgcatgctca tgagtgagcc cggcgacacc
     2521 ggcggcgaaa agaagaatga gcacgagcgg gttggagaag ggcttggcga gaccggcgat
     2581 caactgcagt ggtcgcggct cctcgatccg attgggccca tatttcgcgt ggcgagcggc
     2641 ggcctgttcg gcccgcaagc cgttggcgga cgtccccagg gttgcgtaga tttcgtcgag
     2701 agctcggcgc gacggatcaa cctcgcccag accagagtca gcgtatttgt tcattctccg
     2761 agctcgccga agcgcactgg gttgagatcg gaccccgtaa tcataagcgc ggagcggggt
     2821 ctgggtgctg gtccttcgcc ttacatccgt catccgtttc ggcgcgagaa gtcgtccacc
     2881 cgtctcataa caacgcttgc ttcccggatt ggcaccgaga gcttgtagtg cagcagcatc
     2941 gggttatttg gggccgtcaa cggcggatcc gctatcgctt tgaggactta cctatggggc
     3001 agagccaatc tcgaggagtg aaggtccaag gcttgctaca gagtggggca gtggctcacg
     3061 cattgctccg gggcgaccgc gccgtgaaac ggctgctgct gccggtcaca tcggcgctgc
     3121 tcgtcgccgg cggaatagcg ctgctttacg gccgcgcgga gctttccagc tcattttggc
     3181 tggttggaac ggctgtcgtc cttgccgtac tcctcgtaga cgtagcgctg actttcagcc
     3241 gcggcgagta cgggctcgac ctcattgctg cgctcgccat ggcgggcacc ctcgttctcg
     3301 gcgagcatct cgccggcatc atagtggcgc tgatgttcac cggcggcgag gcgctggagg
     3361 gcctggccca gaggcgggca aggcaagaga tgacggcgct gctaagtcgg ttgccccgaa
     3421 ccgcagcgcg ctcctcggac ggccagctcg aggaggtcgc actggaagag ctcaaggtag
     3481 gggatcgtat cctggtgcgc cgcggggagg ttgtccccgt cgacgggacg gtaaaaagcg
     3541 atgcggccgt cctcgaccaa tccgctctca ccggggaggc gctgccggtt cgccgcgcgg
     3601 ccggggagcc ggtgatgagc ggctccacca acgccgggga tgccttcgag ctgcaggcta
     3661 ccagcaccgc ttccgacagc acatacgcgg gtatcatccg tctcgtcgag gcggcacagg
     3721 cggcgaaggc ccctatgtcc cgcctcgccg acaagtacgc gctggtcttt cttgccatca
     3781 ctcttgccat ggcaggcgga gcatggctgc tgagcggcga tccggtaagg gcgctcgccg
     3841 tgctcgtcgt cgccacgcca tgcccgctca tcctcgccgt tccggtagcg atcatttcgg
     3901 gcgtgtcgcg ctgcgcaaaa aggggtgttc tcgtcaaggg tggtggtgca ctggagctgc
     3961 tcgcccgcat caagacggtc ctcctcgaca agacgggtac catcacggac ggccgcgcac
     4021 gcctcatcga gatgaaggcc aggacagatc tcgacccgct ggaggtgctg cgcctcgccg
     4081 cctccctcga tcaggggtcg caccacgtga tcgcgcaggc gctcgtctcg gcggcgcgcg
     4141 agcggggtct cgctctcgag accccgacgg gcgttcgcga gctgccgggc ttcggcgtga
     4201 gcgggagagt cggcaaccac tcactggcgg tcggcggatg ggagttcgtg aaatcgaccg
     4261 ttgaagccga cgggttcact gacgagattg atacctggat ccggcgcgat gggacggtcg
     4321 cagtcgcggc cgccatcgac gggaagctcg cgggggcctt tctcctcgcc gaccaggtga
     4381 gacccgaagc cggcactgta ctgaggaact tgcgcgacgt cggcgtcgat cggatcgttc
     4441 tcgtcacggg tgacagggcc gatctggcag ccagcgtcgc cgctttcctc agcttcgacc
     4501 acgttgcgag cgagctgcag ccgcaggaca aaacaagagc cgtctacgag gagaaaagcc
     4561 gcgggccggt gatgatggtc ggggacggca tcaacgatgc gcccgcgctt gccgcagccg
     4621 atctcggcgt cgccatggga gcgcgcggtg cggcggcctc gtcggaggcg gccgatgtcg
     4681 tcattctcgt cgaccggctc gatcggctcg tagacgctgt tcgaattgca cgccggtcgc
     4741 gtggaatcgc cttgcagagc gtccttgcag gcatggggct ctcaggtgcc ggaatgttcg
     4801 ctgccgcctt tggctatttg agccccgttc agggtgcgct tctgcaggag ggcatcgatc
     4861 tgctcgtcat actcaacgcg cttcgggcgc taggtgtgcc gctgtgggtg cggctgaagc
     4921 ctcgcacgtt gagcagcgag gagcttcagg ctctcgaagc cgagcaccgc agtctggcgg
     4981 aggttgtcga tagcatccgc tacactgccg accgcatccg gcatctctca ggaccggacg
     5041 tcgcacggga gctcgcagct ctcgaaactg tcttgcgtga gcggctgctt cctcatgaga
     5101 ggcgggagga cgagcaggtc tacagccgca tccggaggag cgctggcgat cccaacgcct
     5161 tggccgggat gagccggacc cacatggaga tccagcgcca ggtgcatgct ctcacgtcgc
     5221 tgcgaaaggc gctccccgtg gctggtccca acagcacgca gcggtacgag atcgagcggc
     5281 tcctccacgg cctcgaggcc attacgcggc tgcatttcgc tcaggaggac gagatctacc
     5341 gctcgctcga atttgactga gtcggcatgc atccgcagcc cccgccagca gcgtgaccga
     5401 gcagcgccga aacagcccgt aggtcagccc aagttcgcct gccgtagttg ccatcgcaaa
     5461 tccgtgttgc agtccatgca gcgttggatc ggccagcgcg ctcgatcctc gagggaatgg
     5521 tcacgccgat tgagcgcgct atcgcgcgac tcaagcatgg agcggttgac cgagcgccgc
     5581 agcagcggct tcgcgcaaac attcgctcaa ggtcgggtgg acgtgaatgg tgccggcgac
     5641 gtcttcgaga cgtgcgccca tctccagcgc caatgcgaaa ctgccggaca gctccgacac
     5701 gccctggcca acggcctgaa ttccgaggat cacgtgattg tcggtgcggg cgacaatgcg
     5761 cacgaatccg tcctccgatc caagcgtcgc ggcacggccg ttggctgcaa agggaaaggt
     5821 gccgaccttg attggtaacc ccgccgaccg ggcttcatcc ggcgacagcc ctgcgctggc
     5881 aatctcagga tcggtgaagc aaacggccgg aattgcgacc ttgtcgaacg accgtttgtg
     5941 gccggcgatg atttcggcca ccatctcccc ttgcgcaacg gcccggtgct cgagcatcgg
     6001 ctcgccggtg acatcgccga tcgcccagac gttccgcatc gatgtgcggc actgatcgtc
     6061 gatccagatg gccgtgccgt tcatctccag atcgagctcg gccagtccgt agccctcgag
     6121 ccgcggccgc cgccccacgg tcatcagaat tttgtcagcg ctgatacgct cttcctcgcc
     6181 gccgtccggc atgactttca gcgccgtgcc cgcggaatcg aggcccaatg ccttcgcctt
     6241 gagcaacacg cgaaccccca gcttctcgag gcgcgacagc accggtcggg taagctccgc
     6301 gtcgtactgc ggcagaatgc gttcggtcgc ctcgaccacg gtgaccacag agccaagctt
     6361 ggcgaaagcc gtgccgagct ccaggccgat gtatccgccg cccacgacga ccagacgctg
     6421 tggcagcgta tcgaacgaaa gagcttccgc ggacgatacc accgggccgc caaacggcag
     6481 gttcggcaga gatacgggtt cggaccctgt tgcgatgacc acgtgctcag ccgtgatcat
     6541 ctggtcgccg gtctcggttt cgacgaggca cgtcttcccg tcgcgcagaa cgccggtgcc
     6601 ggtcaccacc ttgaccttgg cccggcgcag aaggccgaat acgccgcccc gcagttttga
     6661 aacgatgccg tccttccagg ccattgtttc aggcaaatcg aacgtcggct cggctacccg
     6721 caccccagga gcagtgccgg cggcaaatcg ccgggcaagg tagaactgat cggcggcgtg
     6781 gatcagcgcc ttcgacggga tgcagccgat gttgaggcac gtcccgccga gatgccgggc
     6841 ttcgaccagc accacgtcga gcccgagctg accggcacgg atcgcacaca cgtagcctgc
     6901 cggtcctccg ccgagcacga gcacggtgca ggagatcggc tccatctacg tctccatgaa
     6961 aattttggcc ggtgactcga gcagcgatcg cagccgctgg atgaattggg cggcgtccca
     7021 gccgtcgatg atgcggtggt cgaacgagga ggacaggttc atcaccttgc gcggctcgaa
     7081 gctcgtcccg cgccacaccg gccggatcgc gatcttgttg acgccgatga tcgccacctc
     7141 gggcgcattg atgacgggtg tcgatgcgag gccgccgagc ggaccgaggc tggtgattgt
     7201 gatcgtcgac ccggcaagct cttcgcggct tatggtgccg gagcgcgccg cctcgctgag
     7261 ccgctgcacc tcgctcgcgc agtcccagat cgtacgcgcc tccgcatgcc gaaccgtggc
     7321 gaccatcaag ccgttcggcg tttgaatggc gataccgatg tgcaccccac cataacgatg
     7381 gagtatgtca gtatcgtcgt cgtagcgcgc gttcatctgc ggatagtcac gcagggctac
     7441 gacgagggcg cgcatgagga atggcagcac ggtcagcttc ggctggccag gcttcctgtt
     7501 tgcattcacg tctgcgcgca gcgcctcgag gtcggtgacg tcgacctcct cgatgtagga
     7561 gaagtgcgga acgcggcgct tcgtatcttg catccgctgg gcgattttgc g
//
LOCUS       DASVDU010000057        11288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1662018, whole genome shotgun sequence.
ACCESSION   DASVDU010000057 DASVDU010000000
VERSION     DASVDU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662018"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..989)
                     /locus_tag="VF226_03875"
     CDS             complement(24..989)
                     /locus_tag="VF226_03875"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7073155.1"
                     /translation="MPQRVLEGEQARAANDDLPLSRPGRRQGRGTNTRPAFDRKVIAL
                     VYDFDGTLSPRPMQEYTFLPKIGEDPKAFWAESNRLAREQAADPLITYMHLMYKKAKA
                     KGVRIDREDLVALGRGVDLFPGVTEWFEAIGEYVKIRSETNGVTLRHYLISSGLTEII
                     EGTAIYRHFHNVFASEYWFDPYDLPYPKRVITDTGKTQYLFRINKGIEDLRDNINQHM
                     PERDRPIPFANMIYFGDGDTDVPSMAVMRKNGGHAIAVYPPGKPKAKCIELFKANRID
                     FYAAADYRRGSDLFRRTCLLIDRILADIRVQEEVWRIEREVVRGR"
     gene            complement(1117..2664)
                     /locus_tag="VF226_03880"
     CDS             complement(1117..2664)
                     /locus_tag="VF226_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7073156.1"
                     /translation="MSNIRAALSAVAMSVAAGAGWQGPASAAGLPAISQPDSIWLLAQ
                     AQRRGEGNQGEEDERKGRRDGHGGERRDAQPRRADPPKDVRRDAKSTPDRRENDRSRR
                     KEAPRIKAQPQPKIINTERRDARDERREDRKDAREDRREQRREVKEDRREDRKEVRDE
                     RRDAKEDRREDRKDARQERREDRRDAKQDRRDERRDAKEDRRDDRKDARIFRRLEDLK
                     SQRRERAEDGGRRKIIEEADKRIIVREGNRMIIRHDETERLRRRFKDAHIERRRDGGR
                     IARFVRPDGVHIISEYDERDRLLKRYRRHPDGRIVVIIDNTRHYHGPGLLGGLILGTF
                     LDLAPPVIRIPREKYIVDYEDASYEDVYEALSAPPVDDLDRDYSLDEIRYNYALRDRM
                     RRVDLDSINFEFGSWEVSEDQYYKLERIADAMNRLLDRNSEEVFLIEGYTDAVGSEED
                     NIILSDHRAEEVASILSEEFGVPAENLVTQGYGEQFLKIDTQGPERANRRVSVRRITP
                     LLSRAGP"
     gene            2970..3371
                     /locus_tag="VF226_03885"
     CDS             2970..3371
                     /locus_tag="VF226_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7073157.1"
                     /translation="MSESGKDHGSGRGEISREDREALKARAAGIGARLEQVKARRAPQ
                     LDPAARGAAMGQAFKIAIELVVGVLFGGLVGWFLDGYFGSRPWLLIVFLVLGFAAGMM
                     NVVRTARQMQSAAEPLQRAAPSVRPDDEDEE"
     gene            3419..4132
                     /locus_tag="VF226_03890"
     CDS             3419..4132
                     /locus_tag="VF226_03890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7073158.1"
                     /translation="MHQFEINRIAEFQLFGFDASFTNSSLFMVIGCALIVGFLALATN
                     GRSLVPSRLQSVAELSYEFVANMVRENVGLAGMKYFPFVFTLFAYILVMNLLGLIPYS
                     FTVTSHIIITFALAAFVFVLVTVVGFARHGLGYLKLFVPEGVPFWLMPLIVPIELISY
                     LIRPISLSVRLFANMMAGHTMLKVFAGFVVSLGVFGFAPLLFTIAFTGLEFIVAFLQA
                     FIFAILTCIYLNDAVNMHH"
     gene            4204..4428
                     /locus_tag="VF226_03895"
     CDS             4204..4428
                     /locus_tag="VF226_03895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX7073159.1"
                     /translation="MDPTAAKFIGAGLACFALIGAGIGIGNIFGNYLSGALRNPSAAP
                     GQFTNLLIGFALAEATGLFGLLIALIILFG"
     gene            4503..5051
                     /locus_tag="VF226_03900"
     CDS             4503..5051
                     /locus_tag="VF226_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEX7073160.1"
                     /translation="MLASLVVLAAAAPEATGEGGGLPQLNASDFAPQLIWLAITFGAL
                     YFILSRWSLPRVAEVIEERRERIQRDLDEAERLKTETEKAIAAYEQELAAARGRASTI
                     ARETRERLAAEVEEERARVDAQVNAKLAEAEKRISEMKSAALAQVNQIATDTAGAIVE
                     KLIGAEVSVDDARRALEPVPGE"
     gene            5057..5548
                     /locus_tag="VF226_03905"
     CDS             5057..5548
                     /locus_tag="VF226_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX7073161.1"
                     /translation="MSIFATAEFWVAVAFFGFVGLLIYYNAPQLITKALDERADAIRR
                     ELDEARRLREEAQQLLADYQRKSREAEEEASAIIEQARREAEALAAETRQSLKESLER
                     RSKLAEEKIARAEAQALNEVRAAAVESAIAAAERILKRKVTPEVNARLVEQSINEAKR
                     KLN"
     gene            complement(5644..6861)
                     /locus_tag="VF226_03910"
     CDS             complement(5644..6861)
                     /locus_tag="VF226_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7073162.1"
                     /translation="MDQRAGWEPRYAHRAARMRASEIRELLKLLDRPDIISFAGGIPD
                     PALFPVEAAREAYAECLGPSAQQALQYSVSEGYLPLREWIAGYMHRLGVPCTADNILI
                     TSGSQQGLEFLGKLLISPGDTVLTLAPTYLGALQAFSAFEPRYDGLRPEDGNRTAASY
                     SEAAGDTREVKLAYVVPDFANPTGETLSRPARDRLLDLAEELDIPVIEDTAYSALRFE
                     GEPQPSLQALDVARCGSIDRSRVISCGTFSKTISPGLRVGWICAAKPIIHRLVLIKQA
                     SDLNSALLNQMVMYRLAERCFDAQVERARPHYRGRRDAMLAALEAHMPAGVSWTRPEG
                     GLFVWLTLPERIDTTSLLKRAVDEMSVAFVPGGAFFPDGSGRNTARLSYSLPPEATIG
                     EGIARLGALIRQA"
     gene            complement(6990..7451)
                     /locus_tag="VF226_03915"
     CDS             complement(6990..7451)
                     /locus_tag="VF226_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX7073163.1"
                     /translation="MVWGGRDAPKAVLFACTMNAIRSPMAAAILRHLAGQRIYVESAG
                     VRAGDRDAFAEAVMDEIGIDLTRHKPQTLADLHDTTFDLIITLSPEAHHQALELTRTM
                     AVDVEYWPTLDVSALLEIGHRDQILAAYRSVRDGLFERIKRRFQLEGGPSV"
     gene            complement(7453..7950)
                     /locus_tag="VF226_03920"
     CDS             complement(7453..7950)
                     /locus_tag="VF226_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX7073164.1"
                     /translation="MSEFQEQRNGGKARLISIKLDEKSIRRSNPNIDHEREVAIYDIL
                     DANTFVLEGRDDGPYKLTLGVVEDRLVFSVGNESVETIVTHVLSLTPLRRLIKDYFIV
                     CDTYYDAIRSAPPSRIQSIDMGRRALHDEGSRILIERLKGKITVDHDTARRLFTLVCA
                     LHWKG"
     gene            complement(7960..9258)
                     /gene="hisD"
                     /locus_tag="VF226_03925"
     CDS             complement(7960..9258)
                     /gene="hisD"
                     /locus_tag="VF226_03925"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7073165.1"
                     /translation="MPQRLNTADPDFEARFLALLAMKRESAPEVDTAVRQIIADVIAR
                     GDQALIELTRKFDRLELTPETLRISDDEIAQAHRESDPDVIAALQLAHDRIRAYHERQ
                     RPEDARFTDAIGAELGHRWTAVESVGLYVPGGLASYPSSVLMNAVPAQVAGVPRIVMT
                     VPSPSGELNPLVLAAARIAGVSEVYRVGGAQAIAALAYGTETIRPVVKIVGPGNAYVA
                     AAKRQVFGRVGIDSIAGPSEVLIIADRENDPEWIAADLLAQAEHDVAAQSILMTDNAG
                     FADAVCAAVDRQLRELPRGNIAGESWSTFGAVILLRSLEEAPPLADRIAAEHLEIAAR
                     DADVICDRIRNAGAIFLGALTPEVIGDYVAGSNHVLPTARSARFSSGLGVLDFMKRTS
                     ILRLDANALEQLSGAAMTLARAEGLEAHRRSVEIRRSNRS"
     gene            complement(9294..9755)
                     /locus_tag="VF226_03930"
     CDS             complement(9294..9755)
                     /locus_tag="VF226_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX7073166.1"
                     /translation="MGDLKLVAFDAEDLKVISAHLQDAVLQVGDMAYRPAEHRFAAIT
                     NRFDWASATGAHGREPSLERRRTGLRFERVLEAKCQGLDLKSKRQVLELLAIQFEETE
                     PPGGYITLFFAGGPAVRLKVECIEAEMRDLGAAWKTARRPQHPDVETVSEG"
     gene            complement(9969..11258)
                     /gene="murA"
                     /locus_tag="VF226_03935"
     CDS             complement(9969..11258)
                     /gene="murA"
                     /locus_tag="VF226_03935"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7073167.1"
                     /translation="MDRIRIVGGRRLQGTIPISGAKNAALPLMIASLLTQDRLTLKNV
                     PNLADVNLLARILRNHGVDHSVDGKRAGAGQNTGETFHLTARDIVDTTAPYEMVSRMR
                     ASFWVLGPLLARMGEAKVSMPGGCAIGTRPVDLHLMGLKALGAEIDIDGGYVVARAPK
                     GLRGNRIIFPKVSVGATHNVMMAAALAKGETVIENAAREPEVADVASCLVKMGAQIEG
                     IGSSTLRIQGRDRLEGAVHHVLPDRIETGTYAFAVAATGGELFLEGARPELLATALQT
                     LERTGATVSASDGGIRVSRNGAHNEPVTIETQPFPGFPTDLQAQLMALTTTARGTSVI
                     RETIFENRFMHVQELVRLGADIQLHGDTATVRGVKSLRGAQVMATDLRASVSLVIAGL
                     MAEGETTINRVYHLDRGFERLEQKLSACGAEIERISS"
BASE COUNT         1919 a         3663 c         3570 g         2136 t
ORIGIN      
        1 tcccagcctt gaatttcgat gcgctaccgc cccctcacca cctcgcgctc aatccgccag
       61 acctcctcct ggacccggat gtcggccagg atgcgatcga tgagcaggca agtccggcgg
      121 aagaggtcgg agccgcggcg atagtcggca gcggcgtaga agtcgatccg gttcgccttg
      181 aacagctcaa tgcatttggc cttgggcttg ccgggcggat agacggctat cgcgtggcca
      241 ccattcttcc gcatcaccgc catggacggc acgtcggtgt cgccgtcccc gaagtagatc
      301 atgttggcaa acgggatggg ccggtcccgc tcgggcatgt gctgattgat gttgtcgcgc
      361 aggtcctcga tgcccttgtt gatccggaac aggtactgcg ttttcccggt gtcggtgatg
      421 acccgcttcg gatagggcag atcgtacggg tcgaaccagt actccgatgc gaacacatta
      481 tggaaatggc ggtagatggc agtgccttcg atgatctccg tcagccctga cgagatgagg
      541 tagtggcgca gagtgacccc gttcgtttcg gaacggatct tcacgtactc gccgatggct
      601 tcgaaccact ccgtcacgcc ggggaagaga tcgaccccgc ggcccagcgc caccaggtcc
      661 tcacggtcga tgcgaacacc tttggccttc gccttcttgt acatgagatg catgtacgtg
      721 atgagcgggt cggcggcctg ctctcgcgcg agccggttcg attctgccca gaaggctttc
      781 ggatcctcgc cgattttcgg caggaaggtg tactcctgca tgggcctggg cgagagcgtg
      841 ccgtcgaaat catagacgag cgcgatgacc ttgcggtcga acgccggacg ggtatttgtc
      901 ccccgaccct gccgcctgcc tggcctggac aatggcaggt cgtcattggc cgcccgggcc
      961 tgctcacctt ccagcacgcg ttgcggcatg atccggctct tttctactca cgacccctcg
     1021 cgaacagaaa caagatgatt cgcagggcgg aatgaagtcg gcctccggcg acttgccgga
     1081 ggccgatcgg caatatgcct cattgcggtt gtgcgctcac gggccggccc gcgacagcag
     1141 cggcgtgatg cggcgcacgg agacgcggcg gtttgcgcgc tccggcccct gcgtatcgat
     1201 cttcaggaac tgctcgccat atccctgcgt gacgagattt tcggccggca cgccgaactc
     1261 ctcggagagg atcgaggcga cctcctccgc ccggtgatcc gaaaggatga tgttgtcctc
     1321 ctcggacccg acagcatcgg tgtagccctc gatcaggaaa acctcctccg aattgcgatc
     1381 gagcagccgg ttcatcgcat cggcgatccg ctccagcttg tagtactggt cctccgatac
     1441 ctcccacgag ccgaactcga agttgatgga gtcgagatcg acccggcgca tgcgatcccg
     1501 gagggcgtag ttgtagcgga tttcgtcgag cgaataatcg cggtcgagat catcgacggg
     1561 cggggcgctc agggcctcat agacgtcctc gtaagaggca tcctcgtaat ctacgatgta
     1621 cttctcgcgc ggaatgcgaa tgaccggcgg cgcaaggtcg aggaaggtcc ccaggatcag
     1681 cccgcccagc aggcccggcc cgtggtaatg acgggtgttg tcgatgatga cgacgatgcg
     1741 gccgtcgggg tggcgccggt agcgcttcag caaccgatcg cgttcatcat actcagagat
     1801 gatgtgaacg ccgtcgggcc gcacgaaacg cgctatcctg ccgccatcgc gccgccgctc
     1861 gatgtgcgcg tctttgaagc gccggcgcaa ccgttccgtc tcgtcatgac ggatgatcat
     1921 acggttgcct tcgcggacga tgatgcgctt gtcggcctcc tcgatgatct tgcggcggcc
     1981 gccgtcctcg gcccgctcgc ggcgttgaga tttcaggtcc tcgagccggc ggaaaatgcg
     2041 tgcatccttc cggtcgtcgc gacgatcctc cttggcatcc cgacgttcat cccgccggtc
     2101 ctgcttagca tcgcgccggt cctcgcgccg ttcctggcgc gcatcctttc ggtcctcgcg
     2161 gcggtcctct ttggcgtccc ggcgctcatc gcggacctct ttccgatctt cgcgccgatc
     2221 ctccttcacc tcacgccgct gctcgcggcg atcctcccgc gcatccttac ggtcctcacg
     2281 ccgctcatcg cgagcatccc ggcgctcggt attaatgatc ttcggctgcg gttgcgcctt
     2341 gatgcgtggc gcctccttac gccgcgaccg atcattctcg cgccgatcgg gagttgactt
     2401 ggcatcccgt cgaacatcct tcggcggatc ggcgcggcgc ggctgcgcat cgcgccgttc
     2461 cccgccatgc ccatcccgac gccccttccg ctcatcttcc tcgccctggt ttccttcccc
     2521 gcgcctttgg gcctgagcca aaagccagat cgagtccggc tgtgagatgg ccggcaggcc
     2581 tgcggctgaa gccggacctt gccaaccagc acctgccgcg accgacatcg cgacggcgga
     2641 aagtgccgct cggatattcg acattcctgt gcgctccttg ggctgaaagt ctctcgatat
     2701 tgtggcgcct gaaaattgtg acgcttgttt gttgtcgagg cgactcaatg gcacgctggc
     2761 gtatcagatg gcccgcttgc gagtgtccgg aaaggcgcca tccgtgtgcc agacgaacag
     2821 cgcaccctgc gcggtttcgc cgtacccgcg ccacctccaa cgggcgtggc gttttcttga
     2881 cacccctggg ggcccccact aaggtgcgcg ccgacgaata acccgccgtg cttcggccca
     2941 acccaaaaag agttcgggca ggcgttgcca tgtctgaaag cggcaaggac cacggctcgg
     3001 ggcgcggaga gataagccgg gaggatcgtg aggcgctgaa agcgcgcgcg gccggaattg
     3061 gtgcccgcct cgagcaggtt aaggccagac gggcgcctca gctcgaccct gctgcgcgtg
     3121 gggccgccat ggggcaggcg ttcaagatcg ccatcgagct ggtcgtgggc gtcctgttcg
     3181 gcggattggt aggctggttc ctcgacggct atttcgggtc gagaccttgg ctgctgattg
     3241 tgtttctggt gctcggcttc gcagccggca tgatgaatgt ggttcgaacc gcccggcaga
     3301 tgcagtcggc agcagagccg ttgcagcggg cggcaccgtc tgtgagaccg gacgatgagg
     3361 acgaagagta gggggctaga aggtggcgac tggcgcggct gaaggggctc acggccccat
     3421 gcatcagttc gagatcaatc gaatcgccga attccagctg ttcggctttg atgcctcgtt
     3481 cacgaactcc tccctgttca tggtgatcgg ctgcgcgctg atcgtcggct ttctcgcgct
     3541 ggcgacgaac gggcgctcgc tggtgccctc gcgcctgcaa tcggtcgccg agctctcata
     3601 cgagttcgtc gccaatatgg tccgcgagaa cgtcggcctg gcggggatga agtatttccc
     3661 cttcgtcttc accctctttg cttacatcct ggtgatgaac ctcctggggt tgatccccta
     3721 ctcgttcacg gtcaccagcc acatcattat cacgttcgcg ctcgccgcct ttgtgttcgt
     3781 gctcgtcacg gtggtcggct ttgcgcgcca cgggctcggc tacctgaagc tgttcgtgcc
     3841 ggagggcgtg ccgttctggc tgatgccgct catcgtgccg atcgagctca tctcctacct
     3901 tattcgcccg atcagccttt cggttcgtct gttcgccaac atgatggcgg gccatacgat
     3961 gctgaaggtg ttcgcgggct tcgtggtcag cctgggcgtc ttcgggttcg cgccgctgct
     4021 gttcaccatc gccttcacag gcctcgagtt catcgtggcg ttcctgcagg cgttcatctt
     4081 cgccatcctc acctgcatct acctgaacga cgcggtgaac atgcatcact gatcccgcgg
     4141 gcatggggct gccattcccg ggtacttttt ctgcaactct gcgacctcaa agggagactg
     4201 taaatggatc cgacggctgc caaattcatc ggtgcgggcc ttgcctgctt cgccctcatc
     4261 ggtgccggca tcggtatcgg caacatcttc ggaaactatc tgtcgggcgc actgcgcaat
     4321 ccgtcggcgg ccccgggcca gttcacgaac ctgctgatcg gcttcgccct cgccgaggcg
     4381 acgggtttgt tcggcctact tatcgccctt atcattctgt tcggctgatc tgaggatcga
     4441 tcgcgaggcg taacacaggt tcacccacct cgcgatcgtc cccactccga ccggagacta
     4501 tcatgttagc tagccttgtc gtgcttgccg cggcagctcc agaggcgaca ggagaaggcg
     4561 gtgggctccc gcagctcaac gcatcggact tcgcgccaca gctcatctgg ctcgccatta
     4621 ccttcggcgc gctctacttc attctatcgc gctggtcgct gccccgcgtc gccgaggtca
     4681 tcgaggagcg ccgcgagcgc atccagcgcg atctcgacga agcggagcgc ctgaaaaccg
     4741 agacggaaaa ggcgattgcc gcctacgagc aggagcttgc cgcagctcgc ggccgcgcca
     4801 gcacgatcgc ccgcgagacc cgcgagaggc tggcggccga ggtcgaagag gaacgtgccc
     4861 gcgtggatgc ccaggtcaac gccaagctgg ccgaggcaga aaagcgcatt tccgagatga
     4921 agtccgctgc gttggctcag gtcaatcaga tcgcgacgga caccgccggt gccatcgtcg
     4981 agaagttgat cggtgccgag gtcagcgtgg atgacgctcg ccgcgcgctc gagccggtgc
     5041 ccggggagta acgtccatgt cgatcttcgc aacggccgag ttctgggtcg cggtcgcctt
     5101 cttcggcttc gtcggcctgc tcatctatta caatgctccc cagttgatca cgaaggcgct
     5161 cgacgagcgc gccgacgcca tccgccggga gctcgacgag gcacgccgcc tgcgtgagga
     5221 ggcccagcag ctccttgccg actaccagcg caagagccgc gaggccgagg aagaggcgag
     5281 cgccatcatt gagcaggcca ggcgtgaggc cgaggcgctt gccgccgaga cccgccagag
     5341 cctgaaggaa agcctcgaac gcagaagcaa gcttgccgag gagaagatcg cccgggccga
     5401 ggcccaggcg ctcaatgagg tacgggccgc ggccgtcgaa agcgccattg ccgccgctga
     5461 gcgcatcctc aagcgcaagg tcacgccgga ggtcaacgcg cgcctcgtcg agcaaagcat
     5521 caatgaggcc aagcgcaagt tgaactgaag gttacgtcag gccagccagg aggccgccat
     5581 ggcttggaat gaagagcccc gatctccagg tgtggatcgg ggtttttcga ttgacgcctc
     5641 gagtcaggct tgccgtatca gcgctccaag acgtgcgatg ccctcaccga tcgtggcttc
     5701 gggcggcagg gagtagctga gccgggcggt attacggccg cttccgtccg ggaagaatgc
     5761 acccccgggg acgaaggcca cactcatttc gtcgaccgcc cgtttcaaca gcgatgtggt
     5821 atcaatcctc tctggcaagg tcagccagac gaacagccct ccttccggcc gcgtccagct
     5881 gacgcctgcg ggcatgtgcg cctcgagtgc ggcaagcatg gcatcccggc ggcctctata
     5941 gtgcgggcga gcgcgctcca cttgtgcgtc gaagcacctt tcagcaagcc gatacatcac
     6001 catctggttg agaagcgcgc tgttgaggtc ggaagcctgc ttgatgagca cgagtcggtg
     6061 gatgatcggc ttggcagcgc agatccagcc gacgcgcagt ccaggtgaga tcgtcttcga
     6121 gaacgtcccg caggaaatga ctcgcgagcg gtcgatgctt ccgcagcgag cgacatcgag
     6181 cgcctgcaac gacggctggg gctcgccttc gaaccgcagg gcggaatagg cagtatcctc
     6241 gattactggg atgtcgagct cctcagcaag atcgagaagc cggtcgcgag cgggccggga
     6301 aagcgtttcc ccggtcgggt tggcgaagtc gggcaccaca taggccaatt ttacctcgcg
     6361 agtatcgccc gcagcctcgg aataggacgc tgcggtccga ttcccgtcct ccggcctcag
     6421 cccgtcgtac cgcggctcga atgccgaaaa tgcctgcagc gccccgagat aggtcggagc
     6481 cagagtgagc acggtgtcgc cgggtgagat gagcagcttg ccgagaaatt ccaggccctg
     6541 ctgtgatccg gacgtgatga ggatgttgtc cgccgtgcac ggtacgccca atcggtgcat
     6601 gtagccggca atccactccc gcaggggcag atatccctcg ctgacggaat actgcagcgc
     6661 ctgctgcgcc gacgggccca gacactcggc ataagcctcc cgtgccgcct cgacgggaaa
     6721 cagcgccggg tctggaattc cgcctgcgaa ggagatgatg tccggccggt cgagcagctt
     6781 cagcagctcc cggatctccg atgctctcat gcgcgcagcg cgatgagcgt agcgcggctc
     6841 ccaaccggcg cgctgatcca tccgtcgtcc tcccgatttg gattgccatt tatgtcagca
     6901 atgctgacct ttttgcaagc agacttgacg tctgtcgtcc gccgcgctcc cgcgggagcg
     6961 agaatggggc gatgccaagc cgtcagtggc tagacggaag gcccgccttc cagctggaat
     7021 cgccgcttga tgcgctcgaa caggccgtcg cgtacggagc ggtaggcggc gaggatctga
     7081 tcgcgatggc cgatctcgag caaagcggaa acgtcgagcg tcggccagta ctcgacgtcg
     7141 accgccatgg tccgggtcag ctcgagcgcc tgatggtgcg cttccggcga gagcgtgatg
     7201 atcagatcga atgtcgtatc gtgaagatca gcaagcgtct gcggcttgtg gcgcgtcaag
     7261 tcgatgccga tttcgtccat gacggcttcg gcgaacgcat cgcgatcgcc ggctctgacg
     7321 ccggccgatt cgacatagat gcgctgccct gcaagatgcc tcaggattgc cgccgccatc
     7381 ggcgatcgaa tggcgttcat ggtgcaggcg aacagtacag ctttgggtgc gtccctgccc
     7441 ccccagacca tgtcacccct tccagtgcag cgcgcacacc agcgtgaaga gccgccgcgc
     7501 cgtgtcgtgg tccacagtga tcttgccctt gagccgctcg atcagaatgc ggctgccttc
     7561 gtcgtgcagc gcccgccgtc ccatatcgat cgactggata cgcgatggcg gcgctgagcg
     7621 gatcgcgtcg tagtaggtat cgcagacgat gaagtagtcc ttgatcaggc gccgcagcgg
     7681 cgtcagcgag agcacgtggg ttacgatcgt ttcgacgctc tcgttgccga cactgaacac
     7741 cagccggtct tccacgacgc cgagcgtcag cttgtagggg ccgtcgtcac gaccttcgag
     7801 cacgaacgta ttggcgtcga gaatgtcgta gatcgcaacc tcgcgctcat gatcgatgtt
     7861 ggggttggag cgccggatcg atttctcatc cagcttgatc gagatcagcc gcgcctttcc
     7921 tccgtttcgc tgctcctgaa actcgctcat gccggcacgt cagctacgat ttgatcgccg
     7981 aatctcgacc gagcgccgat gcgcctcgag cccctcggcc ctggcgagcg tcatggctgc
     8041 acctgaaagc tgctccagag cgttggcatc aaggcgcaga atggaggtcc gcttcatgaa
     8101 gtcgaggacg ccaagcccgg aggagaagcg ggcactgcgg gctgtcggga gcacgtggtt
     8161 ggagcccgcc acgtagtcgc cgatcacctc gggcgtgagc gctccgagga agatggcgcc
     8221 ggcgttgcga atgcgatcgc aaatcacgtc ggcgtcgcgc gcagcgatct ccaggtgttc
     8281 tgccgcaatg cgatcggcaa gcggcggcgc ctcctcgagc gagcgaagca ggatgacagc
     8341 gccgaacgtg ctccagctct cgccggcgat gttgcctcgc ggaagctcgc gcagctggcg
     8401 atcaacggcc gcgcacacgg catccgcaaa gccggcattg tcggtcatca ggatggattg
     8461 cgcggcaacg tcgtgctcgg cctgggcgag cagatctgca gcgatccatt cgggatcgtt
     8521 ctcccggtcg gcgataatca gcacctcgga cggccccgcg atggaatcga tgccgaccct
     8581 gccgaacacc tggcgcttgg ctgccgccac gtaggcgttt ccaggcccta cgatcttaac
     8641 caccggccgg atggtctccg ttccgtaagc gagcgcggca atggcctgcg cgccgccgac
     8701 gcggtagacc tcgctcaccc cggcgatgcg ggcagccgca agcacgagcg gattgagctc
     8761 gcccgagggc gacggcacgg tcatgacgat tcgtggaaca ccggccacct gggccggtac
     8821 ggcgttcatc agcaccgagc tcggatagga cgcaagaccg cctggaacgt agaggcccac
     8881 cgactcgacg gccgtccagc ggtggcccag ctcggcgcct atggcatcgg tgaagcgcgc
     8941 atcctccgga cgctgccgtt cgtggtaggc gcggatacga tcatgcgcga gctgcagagc
     9001 cgcaatcacg tcagggtccg actcgcggtg tgcctgcgcg atctcgtcat cgctgatgcg
     9061 cagcgtttcg ggggtcagct ccaggcgatc gaatttccgg gtcagctcga tcagagcctg
     9121 gtccccccgt gcaatgacat cggcaatgat ttgccttaca gccgtatcga cctcgggcgc
     9181 gctctcgcgc ttcatggcaa gcagcgccaa aaacctcgcc tcgaagtccg ggtcggcggt
     9241 attcagcctt tgcggcattc ggcctttacc gcccctcggc ggtctccctc caatcagcct
     9301 tcggagacgg tctccacgtc cgggtgctgc ggacggcgcg cggtcttcca ggccgccccg
     9361 aggtcgcgca tctccgcctc gatgcactcc acctttagcc ggaccgccgg accgccggca
     9421 aaaaagagcg tgatataccc gcccggcggc tctgtctcct cgaactgtat ggccagcagc
     9481 tcgaggacct gccgcttgga tttgaggtcc agcccctggc attttgcctc gagcacgcgc
     9541 tcgaaccgca gtcctgtgcg gcggcgctcc aggctcggct cgcgaccatg ggcgcccgtc
     9601 gcagacgccc agtcgaaacg gtttgtgatg gcggcaaacc ggtgctcggc cggccggtag
     9661 gccatatcgc cgacttgaag gaccgcatcc tgaagatgcg ccgagatcac tttgagatcc
     9721 tcggcatcga aagccaccag cttgagatcg cccattggtt cagttcctgc actttgcggg
     9781 cgatacgcac gaaaggcccg catgggcgag agatagcggc ggtgcggtct gcctgcaatg
     9841 ggtgggacga ttgtaccagc ccggcagccg ccgctgggtg cgaaggacta ccagcgtggg
     9901 ttgattccga aactcaatct caaactttgc cgcgccctgg cgaaccccga accgggataa
     9961 tcgggactct agctgctgat ccgctcgatc tccgccccgc aggccgacag cttctgctcc
    10021 agccgttcga agccgcgatc gagatggtag acgcggttga tggtcgtttc gccctcggcc
    10081 atgaggccgg caatcaccag cgagacggat gcccgcaggt cggtcgccat cacctgggcc
    10141 cctcgaaggc tcttcacgcc gcgaaccgtg gccgtatcgc cgtggagctg gatgtcggct
    10201 cccagccgca ccagctcctg cacgtgcatg aagcggttct cgaagatcgt ctcgcggatg
    10261 acgctcgtgc ctcgcgccgt cgtcgtcagc gccatgagtt gcgcctgcag gtcggtcggg
    10321 aagcccggga agggctgcgt ttcgatcgtg acaggctcgt tgtgagcgcc gttgcgcgac
    10381 acacggatac cgccgtccga tgccgaaacc gtagcgccag ttcgctccag cgtctgcaag
    10441 gcggtggcca ggagctcggg acgggcccct tcgaggaaga gctcgcctcc ggtcgccgcg
    10501 acggcgaagg cataggtgcc ggtttcgatc ctgtcgggca gcacgtgatg aacggcgccc
    10561 tcgagccgat cgcgaccctg gatcctcagc gtcgagctgc cgatgccctc gatctgcgcg
    10621 cccattttca cgaggcagct cgccacatcg gcgacctccg gctcgcgcgc cgcattctct
    10681 atgacggtct cccccttggc caatgcggcg gccatcatca cgttgtgagt cgcgccgacc
    10741 gataccttgg ggaagatgat cctgttgccc cggagcccct tgggcgcgcg cgcgacgacg
    10801 tagccgccgt cgatgtcaat ctcggcgccc agcgccttga gccccatcag gtgcaggtcg
    10861 acggggcgcg tgccgatggc acagccgccg ggcatcgaga ccttcgcctc gcccatgcgg
    10921 gccagcagcg gacccaggac ccagaagctc gcccgcatcc ggctcaccat ctcgtagggc
    10981 gcggtggtat cgacgatatc gcgcgccgtg agatggaagg tttcgcctgt gttctgcccg
    11041 gcgcctgccc gcttgccatc caccgagtgg tcgaccccgt gattgcgcag gatgcgcgcc
    11101 agaaggttca cgtcggcgag attgggcacg ttcttgagcg tcaggcggtc ctgcgtcagc
    11161 aggctcgcga tcatcagcgg cagagcggca ttcttggcgc ccgagatggg gatggtgccc
    11221 tggaggcgac ggccaccaac gatcctgatg cggtccatga actttccgtg cgtgatacgt
    11281 gagccgaa
//
LOCUS       DASVDU010000058        11169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1080824, whole genome shotgun sequence.
ACCESSION   DASVDU010000058 DASVDU010000000
VERSION     DASVDU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080824"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VF226_03940"
     CDS             <1..561
                     /locus_tag="VF226_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NAD(+) reductase"
                     /protein_id="HEX7073168.1"
                     /translation="PNHDSRLILVATNTGFAPIWSIAVAALRENPHRMMMIIAGGRTV
                     ESLYMAPALVQLARFPNVLVIPVCSTPQTVSSAVQLGRPTDFLPNLLPSDVLYVCGAP
                     RMVDSVKMIAARAGATCYTDPFLPSNDDKVAESVLIRATGWLAETSRQIGRIALDRNR
                     NRPQRPIQAYRAAEASVRSEYRSERA"
     gene            605..1603
                     /locus_tag="VF226_03945"
     CDS             605..1603
                     /locus_tag="VF226_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX7073169.1"
                     /translation="MLCFCGVLTIFGLGAGGSVASDPAKIVGPNACAECHKQEAAAWK
                     ETHHFKTFREMPRRKEANEIAKRMGVQRIRSESLCLSCHYTVQQKGSNKQPIAGISCE
                     SCHGAGEDWIKVHSQFSGKTETTETKAEKAARWKLAESKGMIRPSSIYQLAKNCYGCH
                     VVPQEDLVNKGGHPAGSPFELVSWSQGEVRHNTWHSKGKKNVPASPARKRMLYLVGLG
                     VELETGLRAVARATDRKFYAFEMARRVDRARRQLAAAAKAVPGVPEIAKMVDYAYSAG
                     LKLNNERYLTAAADGVSKLLMSIIEKYDGSTMAGLDSLIPGPDQFKGTAKGVGATN"
     gene            1685..2590
                     /locus_tag="VF226_03950"
     CDS             1685..2590
                     /locus_tag="VF226_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073170.1"
                     /translation="MRLAARRTSGASIAGRGWGTARTFTSKTVQGFLIAALSGGFFWL
                     VWIYGNGLRDPRYLDGWVLAGGMSLQLYFHIAIKTTSLSPKSAMCWRKVHIFLGYLLI
                     AAFLAHSDFSLPDTGFEWALWVAFVLVSLSGIFGVYLAWSLRAKHAIDESISYDRIPA
                     RRAELARDVHAAVTSPTPDANLIALPTPPYDAWIGDLYTTHLRDFFQGPRNFAAHLIG
                     SQRPLKQLTDEIDKLSRYVDHKGQEKLAVIKSLVVEKDRLDFARVHLGLTRGWLFVHV
                     PATYGLVVLTVLHVLVAYAFSSGAW"
     gene            2746..5082
                     /locus_tag="VF226_03955"
     CDS             2746..5082
                     /locus_tag="VF226_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073171.1"
                     /translation="MPGPLASVHGAIEKCDVCHTKSGSGRLSWAHGLIAGDPLADSKA
                     CLTCHKLPDTAFNAHSASPEVLEQSTARLTKIASKTPVPHAARAQSIAFPTDGIVARD
                     LYCATCHQEHKGANFDLNKITNEQCQSCHVVKFDSFDGHHPKFENYPFKRRTPIIYDH
                     AGHFEKHFPEMAQKDPAKRIPATCSTCHNSRQDRRIMAVAPFEQTCSACHLDQITGKE
                     RVSGPKGIAFLSLPGLDLQTLKNKNASIGEWPQDSEAELAPFMKVMISRNERGRALIK
                     AVEDLNLQDLSGANDNQIKAVTDLVWEIKGLFYALIKGKASDVLADLNITGGRAKLSA
                     NLVADLTASIPRDVVAGAQEQWLPNLAAEMAKGPAAASTVQQAEGATGETNATSTQAD
                     SNQESLSTESPNSAAAAAESGGETESPDKVSVSDTDAASSGLASESDGSGAAAVAPRR
                     NPQACLVAVFGQCLVTKESENTGNRIRTNTVTFRQDGVEKRSFAGEPPPPMRAGLKQI
                     AQAAPSEAPSAGEPPRKKAADQTDDLLYPTEEELRAMKARGRNVGNAAQPQGVPGKAN
                     APNAKPAGSTPSAGAVTPVISIESNIDPESWAEYGGWYRQDYAIYYRPTGHKDKFIYS
                     WLFLTGPQAPKGDNSPAAAVFDYLTSKDAQGSCTKCHSVDDIPGKGRVVNFSPPSIES
                     KQGRFTKFVHEPHFGALENRNSGCLTCHNLEKGRPYLKSYEQGDPQTFASNFGAVKKE
                     LCQTCHTSNMARQDCLLCHKYHVDDVITPIIETKIPAR"
     gene            complement(5182..5925)
                     /locus_tag="VF226_03960"
     CDS             complement(5182..5925)
                     /locus_tag="VF226_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017272788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073172.1"
                     /translation="MKLTENTILITGGGSGIGRELALRFNDLGNEVIVAGRRKEALEE
                     TIAGRDRMHAIALDMENPETIRAFAKHVMSKHPSLNVLINNAGVMRFEELTRARELRD
                     AEQMVVTNLLGPIRLTNALIEHLAVQPSAAIVNVTSGLAFVPLSRAPTYSATKAALHS
                     YTVSLREQLEGKIEVIELAPPAVQTDLTPGQATREGYMPLRQYIDEVISLSQHQPTPK
                     EILVERVGFLRWAERDGRFDEAVKMLNSG"
     gene            complement(5954..6754)
                     /locus_tag="VF226_03965"
     CDS             complement(5954..6754)
                     /locus_tag="VF226_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073173.1"
                     /translation="MALTSAAFAAGSATAQPGGKTPATNGRVALVTGSSRGIGAATAK
                     RLAQDGYSVTVNCEKNRELAAGVVKEIEAAAGRAIWVQADVSDPSAVKRLFDETERAF
                     GGVDVVVSNAGIMRLAPFRDMSDADFSRMVDVNQKGSFYVLREAARRVRDGGRIIALS
                     SSITQLRTPTYGPYAATKAAQELFANILAKELEGRLISVNAIAPGVVNTTLFTDGKTP
                     EQIAGFAARTPHKRLGEPSEIADVIAALCSADCAWINGQTVFANGGIV"
     gene            6642..6899
                     /locus_tag="VF226_03970"
     CDS             6642..6899
                     /locus_tag="VF226_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073174.1"
                     /translation="MPLDEPVTSATLPFVAGVLPPGWAVALPAANAADVNAKLVRTVR
                     LGISIFPVIVPSLVFEEQCSVGTLHIGGARCALAKSYAKLA"
     gene            6966..7901
                     /locus_tag="VF226_03975"
     CDS             6966..7901
                     /locus_tag="VF226_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7073175.1"
                     /translation="MQSLKHAVRTYAARHANGDGLALTPVPGLRMMSVGTPRGDLHSV
                     YRPLVCLILQGAKRMTVGTEERVFSAGQSVIVGADMPVIGRIVEASPDEPYLAIAVEL
                     EMAVLREIAVQLGDLPAGGCPETRVLFAEDTTAQVLDCAARLMGLVDRPEAIPVLRPG
                     IIRELHYWLLAGQHGAALRSLAAPDSNVSRLAAAVAILREDYRSRLPVERLAAAAAMS
                     VTAFHKHFKHMTSLTPGQYQKRLRLIEARRLMLDEGFSASSAAFEVGYESVSQFTREY
                     ARLFKAPPKRDILRARSKPSTVGDRVQESKLARPM"
     gene            8231..8692
                     /locus_tag="VF226_03980"
     CDS             8231..8692
                     /locus_tag="VF226_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7073176.1"
                     /translation="MTFKLNVNGTTYDVDVEPETPLLWVLRDTIGLTGTKFGCGIAQC
                     GACTVHVDGEAVKSCSIPVDSVVGTMITTIEGLSEDSSHPVQRAWIEHTVPQCGYCQS
                     GQIMAAAALLRRTPNPTDKEIDEAMAGNLCRCGTYLRIRRAIHRAAELSQA"
     gene            8704..10899
                     /locus_tag="VF226_03985"
     CDS             8704..10899
                     /locus_tag="VF226_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7073177.1"
                     /translation="MAVLETNISRRTLIRSMLATGGGMILGFHVPGVEAAVVAPKPWT
                     GPTDGIEINAWLTIGADGTVTIRVPHTEQGQGALTSVSMMVAEELDVPWESVHAVFAD
                     MNRHVNRGREYIVTTTHGSQLVRMQRRHISQAGASARERLKQAAAEAWGIDRSEVTAR
                     QGILTAGDRRATYAEFASAAATVTLDQEPDIKAPGEWWLLGTEVARLDVPVKVNGSAH
                     YAIDTRLDGMVYAAVKSCPVPWGRLISYDAEAAKKRPGVVAVMEFRAEKGRTGPNHLQ
                     DGIAVVADSWYRAKTALDLVSIEWNFGSEGQVSDETQAAEAKRLWEEAGEVSGHKGDD
                     ALKIIAASDRVISAEYHRPYETHARMEPINATVSVTDERVDVWSPTQDQAAALRIVAD
                     QLGRDTKDVHVHTVFIGGAFGGNGGGNTAVTRQAAVISDKLRRPVKVIWSREEDIAQD
                     KQRPPHYTKLSAAIGENGLPAAIFSRAVWFTFQGVSRHGPATADYAISTMPYRIPNRR
                     HERHNVAAHIPTATHRAPGANQNGFIIEQFVDEMALAGGWDPLEWRLEMTKGLEPWQR
                     VLLKLKEVSGFRTDLPRGEGMGIAVVEDHGSYCGACATVSVSRNGALRVEKIVLVMNS
                     GYIINPLNCAEQMEGAACWELSHALYGGLRLQNGRFTNLNFDNYNLMRIDQMPEVETH
                     FALSEDGWWGGVAEPGGPPTAPAVANAIYFATGKRIRSTPIVKHDLSWS"
     gene            complement(10933..>11169)
                     /locus_tag="VF226_03990"
     CDS             complement(10933..>11169)
                     /locus_tag="VF226_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX7073178.1"
                     /translation="FTIVNIAVLVLRKDVVDHDHFETPTILPVLGAISCAFLVGPWTS
                     RDPVQYAIAGVLLAIGVVLWCLTALMMRRYAAKS"
BASE COUNT         2276 a         3402 c         3390 g         2101 t
ORIGIN      
        1 cccaatcacg acagtcggct tattcttgtc gcaaccaata ccggctttgc gccgatctgg
       61 tcgattgccg tcgctgcgct gcgcgagaac ccgcatcgca tgatgatgat catcgctggc
      121 ggccgcactg tcgagtctct ctacatggct cccgctctcg tgcaattagc ccgctttccg
      181 aatgtgctcg tcatacctgt ctgcagcacg ccgcaaaccg tgagcagcgc agtgcagttg
      241 ggccgaccga ccgactttct gccgaacctg ctgccgagtg acgtgctcta cgtctgtggc
      301 gcgccaagga tggtcgattc ggtcaagatg atcgcggccc gcgccggcgc gacctgctat
      361 acggatccat tcctgccgag caacgacgat aaggtcgcgg agagcgtcct cattcgcgct
      421 acggggtggc tcgctgaaac cagccggcag attggtcgaa tagctcttga tcgcaaccgc
      481 aaccggccgc aacgaccgat tcaggcgtat agggcggctg aagcaagtgt gagaagtgaa
      541 tatcgttctg agcgtgctta accgaccgat gggaactagg ggtcggcgcc cccattggcc
      601 ttttgtgctt tgtttctgcg gcgttctgac gatcttcggc cttggcgccg gcggctcagt
      661 cgcgtccgat cccgccaaga tcgtcggtcc gaatgcctgt gccgaatgtc ataagcagga
      721 ggccgcggcc tggaaggaaa cgcatcattt caagaccttt cgcgagatgc cgcgccggaa
      781 ggaagcaaac gaaatcgcca aaaggatggg cgtccagcgc atacgatcgg agagtttgtg
      841 cctgagctgc cattacacgg tgcagcaaaa gggaagcaac aagcagccaa ttgccggtat
      901 ctcctgcgag tcttgccacg gtgccggtga ggactggatc aaggtccaca gccagttcag
      961 tggcaaaacc gaaaccaccg agacaaaggc cgaaaaggcg gcgcgttgga agcttgctga
     1021 gtccaaggga atgatcaggc cgtcctcaat ctatcagctg gcgaagaact gctacggctg
     1081 ccacgtcgtg ccgcaagagg atcttgtgaa taagggtggt catcctgccg gcagcccctt
     1141 cgagctcgtg tcctggtcgc agggcgaggt acgacacaat acttggcact caaaaggcaa
     1201 aaaaaatgtg cccgccagcc cagcacgcaa gagaatgctc tatctggtcg gtctcggcgt
     1261 cgagctcgag accgggctcc gcgcggtcgc tcgtgcgacg gacagaaaat tctacgcctt
     1321 cgaaatggcg aggcgtgtcg accgcgcgcg caggcagctc gccgccgcgg cgaaagcggt
     1381 gccaggcgtg ccggaaattg ccaagatggt cgattacgct tattcggccg ggctgaagct
     1441 gaataacgag cgctacctga cggctgccgc cgacggtgta tccaagctcc tcatgagcat
     1501 tatcgagaag tatgacggca gcacgatggc tgggctcgac agcctcattc ccggaccaga
     1561 ccagttcaag ggaaccgcga aaggagtcgg cgcgaccaac tgatgccgac cggtggcaga
     1621 gatggcaacc tgacaggctc gatcgggaaa gacctacagc aatggatgat ttcggggggc
     1681 cgccgtgaga ttggcggcgc ggcgtacgtc tggcgcttcg atcgccgggc gcggttgggg
     1741 cacggcgaga actttcacca gtaagacggt ccagggcttt ctgatagcgg cgctttccgg
     1801 cggcttcttc tggcttgttt ggatctatgg caacgggctc cgcgacccac gttatctcga
     1861 tggttgggtg ctggccggcg gcatgagctt gcagctctat ttccacatcg ccattaagac
     1921 gaccagtctg tcgccgaaat ccgccatgtg ctggcgcaag gtccatattt ttcttggcta
     1981 tttactcatc gccgcgtttc ttgcgcattc ggatttctcg ctgccggata ccggcttcga
     2041 atgggcgctg tgggtggcat tcgtgctggt gtcgctgagc gggattttcg gcgtttatct
     2101 cgcctggtcg ctgcgggcca agcacgcgat cgacgagagc atcagctatg atcgcattcc
     2161 cgcccgcagg gccgagctgg cgcgggatgt ccacgcggcc gttaccagtc ctactccgga
     2221 tgcgaacctg atcgccttgc cgacgccgcc ctacgatgcc tggatcgggg atctttacac
     2281 cacacacctg cgggatttct tccaagggcc gcgtaatttt gcggcccacc tcatcggctc
     2341 gcagcgcccg ctcaagcagc tgactgacga gatcgataag ctgtcacgct acgtcgacca
     2401 taagggccag gaaaagctcg ctgtcatcaa gagcctggta gtcgaaaaag accggcttga
     2461 tttcgcacgt gtccatctcg ggctcaccag aggttggctg ttcgtgcatg tgcctgccac
     2521 ttatgggctg gtcgtgctga ccgtgctgca tgtgctcgtc gcctacgcct tttcgtcggg
     2581 ggcttggtga tgcggaagcc agcaccgacc ggatcggtca agccaaaggc cgacagaccc
     2641 acgcccccag gctcctggcg cgaccgtatc gcaattttgc tggcggtcgt gactattctc
     2701 tgcgtcggtt ccgctttcct tctttccagg gatacgcaat tcctgatgcc gggtccgctg
     2761 gccagcgttc atggtgcaat cgagaagtgc gacgtttgcc atacaaaaag cggcagcggc
     2821 agattgagct gggcccacgg cctcatcgcc ggcgatccgc ttgccgacag caaggcctgc
     2881 ctcacctgcc acaagttgcc cgacactgcc ttcaacgcgc acagcgcctc gcccgaggtg
     2941 ctcgagcaaa gcaccgcgcg gctgaccaag atcgcctcga agacgcccgt gccgcacgcc
     3001 gcccgcgcgc agagcatcgc cttccccact gacggcatcg tcgcacgcga tctttattgc
     3061 gcaacctgcc accaggagca caaaggcgcc aatttcgatc tgaacaagat caccaacgag
     3121 caatgccaat cctgccacgt cgtaaagttc gacagcttcg acggccacca tccgaaattc
     3181 gaaaattatc cattcaagcg gcgcacacca atcatctatg accatgccgg ccacttcgag
     3241 aagcatttcc cggaaatggc acagaaggat ccggcaaagc gcatccctgc tacctgttcg
     3301 acttgtcaca acagccgcca ggacaggcgg attatggccg tggcgccgtt cgagcagacc
     3361 tgcagcgcct gccatctcga ccagatcacc ggcaaggaac gggtcagcgg cccgaaaggc
     3421 atcgccttcc tgagcctgcc cggcctcgac ctgcagacat tgaagaataa gaacgcctca
     3481 atcggagaat ggccgcagga ctccgaagcc gagctcgcac cattcatgaa agtcatgata
     3541 agccgcaatg agcgcggccg tgccctgatc aaagctgtag aagacctgaa cctgcaggac
     3601 ctgagcggtg caaacgacaa ccagatcaag gctgtgaccg atctggtctg ggagatcaag
     3661 gggctcttct atgctctgat caaaggaaaa gcgtccgatg ttctagccga cctcaatatc
     3721 accggcggca gggcgaagct gagcgcgaat cttgtcgccg atctgaccgc cagcatcccg
     3781 cgcgatgtcg tcgccggtgc acaggagcag tggctgccca atctcgcggc ggaaatggct
     3841 aagggaccag cagcagccag cacagttcaa caggctgaag gagcaacggg agaaaccaac
     3901 gccacaagca cgcaggccga cagcaaccag gaatcgctct ccacagaaag cccaaattcc
     3961 gcggcggcgg cggcagagag cgggggtgaa accgagagtc ccgataaagt ttccgtttcc
     4021 gatacggatg cagcaagctc cggactcgct tccgaatccg acggtagcgg agcggccgcc
     4081 gtcgcgccaa gacgcaatcc gcaagcctgc ctcgtggccg ttttcggcca atgcctggtg
     4141 accaaggaat cggagaatac tggcaatcga attcgaacga ataccgtgac gttcagacaa
     4201 gacggagtcg agaaaaggtc cttcgccgga gagccgcctc ctccgatgcg cgcaggactc
     4261 aagcagatcg cgcaggcagc tccgtcagaa gcaccttccg ccggcgagcc gccccggaag
     4321 aaggcggcgg accaaaccga cgatctcctc tatcccaccg aggaagagct ccgggcgatg
     4381 aaagccaggg gaaggaacgt cggaaatgca gcccagcctc agggagtacc cggaaaagcc
     4441 aatgccccaa acgccaagcc agcgggctcg acccccagcg ctggcgccgt cacgccggtg
     4501 atcagcatcg aaagcaacat agatccggag agctgggccg aatatggcgg ctggtatcgg
     4561 caggattacg ccatttatta ccgtcccacg ggccacaagg ataaattcat ctactcctgg
     4621 ctgtttctca cgggccccca ggcgccgaaa ggagacaaca gccccgcggc ggcggtcttc
     4681 gattatctga cgagcaagga cgctcaaggg tcatgcacga aatgtcacag tgtcgatgac
     4741 atccctggca aagggcgtgt cgtcaatttc tccccaccct cgatcgagag caagcagggc
     4801 cgcttcacaa aattcgtcca tgagccgcat ttcggcgccc tggaaaacag gaatagcggt
     4861 tgcctgacct gccataacct cgagaagggt cgcccttatc tgaaaagcta cgaacaggga
     4921 gatccgcaaa ctttcgcatc caatttcggt gcagtgaaaa aagagctctg ccagacctgc
     4981 cataccagca acatggcgcg tcaggactgc ctgctgtgcc acaagtatca tgtggatgac
     5041 gtcatcacgc cgatcattga gacgaagatc ccagctcgtt aatgtttgat gcagccgcct
     5101 tttctggcgg ccgcattttt cttgataggc ttgagggaga gatgtcccac ctcgcgcaaa
     5161 tggcggcgcc taacccagtc gtcagccgct gttgagcatc ttcacagctt catcaaaccg
     5221 gccatcccgc tccgcccagc gtagaaagcc tacgcgctct accaatatct ccttgggcgt
     5281 cggctggtgc tgagaaaggc ttatcacctc gtcaatgtac tggcggagcg gcatgtagcc
     5341 ttcccgtgtc gcctggccgg gtgtcagatc tgtctgaaca gcaggcggtg cgagctcgat
     5401 gacctcgatc ttgccttcca gttgctcgcg cagtgacacc gtgtaggaat gcagtgcagc
     5461 tttggtggcg ctgtaggtag gtgcgcgact gagcggtacg aacgccaggc ctgacgttac
     5521 gttgacgatc gcggcacttg gctgaacggc gaggtgctcg atcagggcgt tcgtgagacg
     5581 gatcggtccc agcagattgg tgacgaccat ctgctctgca tcgcgtagct cccgtgctcg
     5641 ggtcagctcc tcgaaacgca tgacgccggc attgttgatc agaacgttga gcgacggatg
     5701 cttactcatc acgtgcttgg cgaatgccct gattgtttcc gggttttcca tatcgagggc
     5761 gatggcatgc atccgatccc ggccggcgat cgtctcctcg agcgcctctt tgcggcgacc
     5821 ggcaacgatc acctcgtttc caagatcgtt gaaccgcaac gccagctccc gaccgatgcc
     5881 ggagccgccg ccggtgatga gaatcgtgtt ctctgtcaat ttcattgtga tgtatcccat
     5941 ctgttgcacg gcgtcagacg atgccgccgt tggcgaagac tgtttgcccg ttgatccagg
     6001 cgcagtcggc actgcatagc gcggcgatga catcggcgat ctcggagggc tcgcccaggc
     6061 gcttatgcgg cgtgcgcgca gcgaagccag cgatctgctc aggcgttttg ccatccgtga
     6121 atagagtggt gttcacgacg cctggggcga tcgcatttac agaaatcagg cgcccctcga
     6181 gctctttcgc gagaatgttc gcaaaaagct cttgtgccgc cttggtagct gcataggggc
     6241 cgtacgttgg ggtgcgcagc tgtgtgatgc tcgacgaaag cgcgatgatg cggccgccgt
     6301 cgcgtacccg ccgggcggcc tcgcgcaaga cgtagaagct acctttctgg ttgacatcga
     6361 ccatccggct gaagtcggca tcggacatgt cgcggaacgg cgcgagacgc atgatcccag
     6421 cattgcttac caccacatcg acgccgccga atgcccgctc agtctcatcg aacagacgct
     6481 tcacggcact cggatcacta acgtccgctt gtacccagat cgcgcgccca gcggccgcct
     6541 cgatctcttt gacgactccc gctgcgagtt cgcggttctt ctcacagttg acggtcacgg
     6601 aatagccgtc ctgggccaag cgcttggccg tggcagcgcc gatgcctctt gacgagccgg
     6661 tcacgagtgc gaccctgccg ttcgtcgccg gcgtcttgcc ccctggttgg gcagtagcgc
     6721 tgcctgctgc aaatgccgcc gacgtcaacg ccaagcttgt cagaacggtc cgtctcggaa
     6781 tttcgatttt tcctgtcatc gtgccatccc ttgttttcga ggaacaatgt tcggtcggca
     6841 cgcttcatat cggtggcgcc aggtgcgcgc ttgccaaaag ctatgcaaaa cttgcctgat
     6901 cctgctggcc acgtgatcat tgccgccccg tgtgctaagt ttgggcgaag tcaaggagag
     6961 agaccgtgca gtcgctcaaa cacgccgtcc gcacctatgc cgcccgacat gccaatggtg
     7021 atgggttggc actgacgcca gtaccgggcc tgaggatgat gtctgtcggg acgccccgcg
     7081 gcgacctgca ttccgtctat cggccgctgg tttgtctcat cttgcaaggc gccaagcgca
     7141 tgactgtcgg aacggaggag cgcgtcttct ccgcggggca atccgtgatc gtgggcgctg
     7201 atatgccggt gatcggccgc atcgtggagg cgagcccgga cgagccttat ctcgcgattg
     7261 ccgtcgaact ggaaatggcg gttttgcgcg aaatcgcagt acagctcgga gatctgcccg
     7321 cgggaggctg ccctgaaacg cgcgttttgt tcgccgagga taccactgcc caagttttgg
     7381 attgcgcggc gcggctgatg ggccttgtgg atcgacccga ggccatacca gttctacgac
     7441 ccggtattat tcgtgagctg cattactggc tgcttgcagg acagcacggc gccgcgttgc
     7501 gctcgcttgc agccccggac agcaacgtga gccgtctcgc cgcggccgtt gcgatcctcc
     7561 gggaggatta tcgatcgcgg cttcccgtcg agcgcctggc ggcagcggca gcgatgagcg
     7621 tcactgcctt tcacaagcac ttcaagcaca tgacctcact aacccccgga caatatcaaa
     7681 agcgccttcg cctcatcgag gccaggcgct tgatgttgga cgaaggtttt tcagcgagca
     7741 gcgctgcatt cgaggtgggt tacgaaagtg tctcgcaatt cactcgcgaa tatgctcgcc
     7801 tcttcaaggc gccgccaaag cgcgacatcc tgcgcgcgcg atcaaagcca agcacggtcg
     7861 gagacagagt gcaggagagc aaactggcga gacccatgta atgtggtgct tgcagctgcc
     7921 gccggatgtg aatttcggag tacgcccgat attctgctca cattcaggag ctgtttttga
     7981 gatcgactgt cccgatctca attaagcaat ttgatccgtt caaaacgcgc ttacttttcg
     8041 agttctcacg aaatagtgaa ctccccactg tcatgataat gctgccataa taggtactga
     8101 ttgaggtcac gggctgcctt gcaatcattc gctagagttg gaacttggct cacggtaacc
     8161 gcgacgcgaa acttgattgc cctctgaccc gctgggatcg gcgttctctc caaacaactg
     8221 ggaggaacac atgacattca agctgaacgt gaatggcacc acctacgatg tggatgtcga
     8281 gcccgagacg ccgctgctct gggttctgcg ggacacgatc ggcctgaccg gcaccaagtt
     8341 cggctgcggc attgcccaat gcggcgcatg caccgtccac gtcgacgggg aggccgtcaa
     8401 atcgtgcagc attccggtgg acagtgtcgt cggcaccatg atcaccacga tcgagggcct
     8461 ttccgaagac agcagccatc cggtgcagcg ggcctggatc gagcataccg tgccgcaatg
     8521 cggctactgc cagtcgggcc agatcatggc ggcggccgca cttttgcggc gcactcccaa
     8581 tcccaccgac aaagaaatcg acgaagcgat ggctggcaat ctttgccgtt gcggaaccta
     8641 cctgcgcatc cggcgcgcga ttcatcgcgc tgcggagctc tcgcaagcct gagggaggac
     8701 gccatggccg tactcgagac caacatttcg cggcgcacgc tgatccgctc gatgctcgct
     8761 accgggggcg gcatgatcct cggctttcac gttcctggcg tcgaggcggc cgtcgtcgcg
     8821 ccgaaaccct ggacgggtcc gacggacggg atcgagatca acgcctggct gaccatcggc
     8881 gccgacggca ccgtgaccat ccgcgtgccg cacacggaac agggtcaggg cgccctcacc
     8941 tccgtttcca tgatggtcgc cgaagagctc gacgtgccgt gggaaagcgt gcacgccgtg
     9001 ttcgcggaca tgaaccgtca cgtcaatcgc ggccgggaat atatcgtcac cacgacgcat
     9061 ggctcgcagc tcgtccgcat gcagcgtcgc catatcagcc aggccggggc gtctgcccgc
     9121 gagcgactga agcaggccgc ggccgaggca tggggtatcg accgctcgga ggtgacagcc
     9181 cgccagggca tcctcacggc cggtgaccgc cgcgccactt acgcggagtt tgcgagcgcc
     9241 gcggcgacgg tgacgctgga tcaagagccg gacatcaagg cgcccggcga gtggtggctg
     9301 ctcgggaccg aggttgcgcg actcgacgtt ccggtgaagg tgaatggcag cgctcattac
     9361 gccatcgaca cgcgtctgga cggcatggtc tatgctgcgg tcaagagctg ccccgtcccc
     9421 tgggggcgcc ttatcagcta tgatgccgag gcggcgaaaa agcgtcctgg cgttgtcgcc
     9481 gtgatggagt tccgggccga aaagggccga accggaccaa accatctgca ggatggaatt
     9541 gcggtcgtcg ccgacagttg gtaccgcgcg aagacggcgc tcgacctcgt ctcgatcgaa
     9601 tggaatttcg gatccgaagg ccaggtcagc gacgaaactc aggccgcgga agcgaagaga
     9661 ctctgggaag aggccggcga ggtcagcggc cacaaaggcg acgatgcgct gaaaattatt
     9721 gctgcatcgg atcgggtcat cagtgcggag taccatcgcc cttacgagac acatgcgcgc
     9781 atggagccga tcaacgccac cgtgagcgtg accgacgagc gcgtcgatgt ctggtcgccg
     9841 acgcaggacc aggcggcagc cctccggatc gttgccgatc aactcggccg cgatacgaag
     9901 gatgtccacg tgcacaccgt cttcatcggc ggcgcgttcg gcggcaatgg cggcggcaac
     9961 acggccgtaa cgcgacaggc tgcggtgatt tccgacaagc tccgcaggcc ggtcaaggtc
    10021 atctggtcgc gtgaagagga cattgcccag gacaagcagc ggccgccaca ttatacgaaa
    10081 ctttcagccg ctattgggga aaatgggctt cccgccgcaa tcttctcgcg tgctgtctgg
    10141 ttcacctttc agggtgtgtc gcgacacggg ccggccacgg cggactatgc catcagcacc
    10201 atgccctatc gcatcccgaa ccggcggcat gagcgccaca acgtggccgc tcacattccg
    10261 acggcaacgc atcgtgctcc gggcgccaac cagaacggct tcatcatcga gcagtttgtg
    10321 gacgagatgg cgctggccgg gggatgggat ccgctcgagt ggcgcctcga aatgaccaag
    10381 gggctcgaac cctggcagag ggtcctcctc aagctgaagg aggtctccgg cttcaggacc
    10441 gatctgccgc gcggtgaagg aatgggaatc gccgtggtcg aggaccatgg ttcctattgc
    10501 ggcgcctgcg cgacggtttc ggtgagccgg aacggggcgc tgcgggtcga gaagatcgtg
    10561 ctcgtgatga acagcggcta catcatcaac ccgctgaact gcgcagagca gatggagggc
    10621 gccgcgtgtt gggagctgag ccacgcgctc tatggcgggc tgcggctgca aaacggcaga
    10681 ttcaccaacc tgaacttcga caactacaat ctgatgcgga tcgaccagat gcctgaggtt
    10741 gagacgcact tcgcgttgtc ggaggatggc tggtggggtg gcgtcgccga gccgggcggc
    10801 cctccaaccg cgccggcggt cgcgaatgcg atctacttcg cgaccggcaa acgcatccgt
    10861 tcgacgccga tcgtgaagca cgatctgagt tggtcgtgag ctgagaggag cggttcgggg
    10921 cggagaggca tctcaggatt ttgccgcgta ccgacgcatc atcaatgcgg tgaggcacca
    10981 gagcacgacg ccgatcgcaa ggagaacccc ggcgatcgcg tactgcaccg ggtcgcgact
    11041 ggtccatggc cctaccagga aggcacacga gatcgcgccg aggaccggca gaatggtcgg
    11101 cgtctcgaag tggtcgtggt cgacaacatc cttccgaagc acaagaaccg caatgttcac
    11161 gatcgtgaa
//
LOCUS       DASVDU010000059         7073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_375125, whole genome shotgun sequence.
ACCESSION   DASVDU010000059 DASVDU010000000
VERSION     DASVDU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375125"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..372)
                     /locus_tag="VF226_03995"
     CDS             complement(1..372)
                     /locus_tag="VF226_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEX7073179.1"
                     /translation="MSNFVEKLTNRWPDVANFLLGLWLVASPWVLGYAGEATAAWNVY
                     IFGAAIAALAAIAFFAQQKWEHWVSLAIGAWLIVSPWVLAYDALQAALWNQIVTGLVV
                     CVLAIWAAASESGFGGLAARS"
     gene            complement(597..1091)
                     /locus_tag="VF226_04000"
     CDS             complement(597..1091)
                     /locus_tag="VF226_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7073180.1"
                     /translation="MNFGSLIPWRSKSQSMARRDDFFDPMFSLRREVDRIFDEFFNGF
                     GSFGDGAGLSPRPVVDIADNDKELVITAEIPGLDEKDLDVSLAGDLLTIKGEKKAEHE
                     ERNGGAYYMERRYGSFSRSVRLPFQVDDQDVEAKYEKGVLTIRIPKPIEMQKAVRRIP
                     IKAS"
     gene            complement(1194..1667)
                     /locus_tag="VF226_04005"
     CDS             complement(1194..1667)
                     /locus_tag="VF226_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX7073181.1"
                     /translation="MATLDFTPLYRSTVGFDRVSMLLSHAMQREESGYPPYNIEKCGE
                     DKYRIVMAVAGFSKDDISVIAERNRLTVRGQLKDRTERTYLHRGIAARSFERHFDLAD
                     YIEVEGATMGDGLLIIDLKRELPEELKPRKIEITAGTITQIEHKKLEGSAEAAAA"
     gene            complement(1824..2471)
                     /locus_tag="VF226_04010"
     CDS             complement(1824..2471)
                     /locus_tag="VF226_04010"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7073182.1"
                     /translation="MTASAADAAGKLDVFYPIVPDVGWLKRIVPLGVRTVQLRIKNAS
                     PDEIRRQILEGLELCTRHGCQLIVNDYWREAIDAGADYVHLGQEDLAGADVPTLKAHK
                     IKIGISTHTPEELETALAAEPDYVALGPIWETKLKVMRYAPQGLDRIREWKARIGALP
                     LVAIGGITPERASSVIEAGAQSASVITDFFTHPDPEARIRTWLEWADGMRQLATA"
     gene            complement(2468..3295)
                     /locus_tag="VF226_04015"
     CDS             complement(2468..3295)
                     /locus_tag="VF226_04015"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX7073183.1"
                     /translation="MNAIDIQQSRAAAPLKVYGTSIGSRLLLGTAHYPSPQILADAVK
                     ASRTEIVTVSVRRETAGGRTGQRFWELIRELGVRVLPNTAGCRTPKEAITTAQMAREL
                     FETDWVKLEVIANDDTLQPDLFGLVEAARVLAEDGFKIFPYTTEDLGAAERLMAAGCE
                     VLMPWGAPIGTGRGLNNKYALKTLRNYFPGVPLIIDAGIGAPSHASEAMEMGYDAVLL
                     NTAVSKAGDPVLMAKAFAAAIEAGRMAFEAGLMEVRDMAEPSTPVAGTPFFDFGGRR"
     gene            complement(3302..3529)
                     /gene="thiS"
                     /locus_tag="VF226_04020"
     CDS             complement(3302..3529)
                     /gene="thiS"
                     /locus_tag="VF226_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX7073184.1"
                     /translation="MDLQSDAGSRLNVFVNGEPLATEARTLAELVIEAGFADTRIATA
                     VNGEFVPERARAGRQLAHGDRIEIVSPRQGG"
     gene            3727..4494
                     /locus_tag="VF226_04025"
     CDS             3727..4494
                     /locus_tag="VF226_04025"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7073185.1"
                     /translation="MSLDRFSLKGRCALITGSSQGIGYAIANGLATAGATVVLNGRDQ
                     AKLQKALTAVQSAGHTAHARAFDVTDSEAATAAVEAIEREIGPIDILVNNAGTTRRMP
                     TEDLPDEIWHEIIRTNLDSVFYMSRAVGRHMIGRKRGSIINICSVMSELGRPTIVPYT
                     ASKGGVKMMTKGLAADWGKHGIRVNGIAPGYFKTPLNEALVKDEKFSSWVESRTPLGR
                     WGEVDELVGAAVFLASDAASFVTGHVLFVDGGMTSVV"
     gene            complement(4506..5792)
                     /locus_tag="VF226_04030"
     CDS             complement(4506..5792)
                     /locus_tag="VF226_04030"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7073186.1"
                     /translation="MALTLLLGVMLALTALGIPLVFSLLGASVFTLFIIRPELPLTVL
                     PQLFIGGIDNFVLLAIALFFLAGELMTASGVIDRILTFARALVGHMRGGLGQVGVVSS
                     VVMAGVSGSAVADAAAVGPLLIRSMKKEGYPASFSAALVETASTLGPIIPPSIPMIIY
                     AVLANVSVGTMFIAGILPGVLIAIGLSTVVYISARRMNLPRSERVSKTVILSETLRAG
                     LALLAPVIIVGGIRGGVFTPTEAGAVATVYVLLIGVFAYRALSFRGTLDALIRAAHGT
                     GAVLVILGASSIFAWIIADQRIGQELAATIRGLDLEPWVLLLALNVIFFVVGMFLDPL
                     PALVILVPVFLPMVTAIGMDPIHFGVMIVLNLVIGLCTPPVGALIYLMASIGDVSPAA
                     VVRASLPFLATLIIVLMLVTYIPAVSLTLPHLIGGH"
     gene            complement(5796..6242)
                     /locus_tag="VF226_04035"
     CDS             complement(5796..6242)
                     /locus_tag="VF226_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073187.1"
                     /translation="MLALLQRVVDGVCAALLAVIAALAFYQVIARYMLGVSTPWGEEL
                     LRLLFVWLVLLGATRTAHMRIDLLEQAVSPRVRQALVVLQTLVAASLLGFMIWHALTL
                     IELTAYDRYTALPVSVQWLYRAMLIGGVLWLVFTVAGVAGRLRGEN"
     gene            complement(6250..>7073)
                     /locus_tag="VF226_04040"
     CDS             complement(6250..>7073)
                     /locus_tag="VF226_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7073188.1"
                     /translation="TNGEVKVSVHPAGELGNDPAILEGVRLGTIDIANTGNPFFTRFE
                     PRLNVLDLPYVFKSYEHAYRIIDGPIGQELIDGLQKHRMQPLAYWEIGFRNITNSRRS
                     IRTPQDLDGLKIRTTPNPAHVEAFKILGAIPTPMPFTEVYLALESGTVDGQENPLAII
                     HSNRFHEVQKHLSMTRHAYTVSITVMNQQKFNSLSEEHRKALLQAAKEAQQLQRKLNR
                     EADKTALEEFRKAGVEVIEEIDTEPFRKAVFEPVKETYVKEYGSEIVDKIIAAGQ"
BASE COUNT         1362 a         2226 c         2243 g         1242 t
ORIGIN      
        1 tcagctccgc gccgcgagac cgccgaagcc ggactcgctg gctgccgccc agatcgcaag
       61 cacgcaaacg acgaggccgg ttacgatctg gttccagagg gcagcctgca gcgcgtcata
      121 cgccaacacc cacggcgaca cgatcagcca cgcgccaatg gccaggctga cccagtgctc
      181 ccacttctgc tgcgcaaaga atgcgattgc agccagggca gcgatcgcgg caccgaagat
      241 gtagacattc cacgctgcgg tcgcctcacc ggcgtatccg agcacccaag gcgacgctac
      301 gagccacagc cccaggagga agttggccac atcaggccag cgatttgtga gcttctcgac
      361 gaagttgctc atgtcacgtt tctcctctct gcgcttcagc aacgtgatcc gcgcgggggc
      421 ggacccatcc gccgccgcgc ggggatgagc tcacggcatg agaaccgcga tgatgaaggc
      481 caggctcagg agcagcatgt tgagtgccca gagcctggac agggtacgcc tcccgacacg
      541 cattcggacc tccttctcac cacggtggtg cggagatggc agtccgacga gcgggatcag
      601 gacgccttga tcggaattcg gcgcacagcc ttctgcatct cgatgggctt cgggatgcgg
      661 atggtcagca cgcccttctc atatttggcc tcgacgtcct ggtcgtccac ctggaacggc
      721 agacgcacgg atcgagagaa ggagccgtag cggcgctcca tgtagtaggc cccgccgttc
      781 ctctcctcgt gctcggcctt cttctcaccc ttgatcgtga ggagatcgcc ggcgagcgag
      841 acgtcgagat ccttctcatc cagaccgggg atctcggccg tgatgacgag ctccttatcg
      901 ttatcggcga tgtcgacgac agggcgcggg cttaggccgg caccatcgcc aaagctgccg
      961 aacccgttga agaactcgtc gaagatgcgg tcgacctcac gacgcagcga gaacatcgga
     1021 tcgaagaaat cgtcccgccg agccatggac tgagatttcg accgccaagg gatcagagaa
     1081 ccgaagttca tcttgcacct ccatatttcc tgatgcttcc ccacgttgca gacaccctgt
     1141 cggcccgctc ggagccgtga gggcttgctg gcgcgggtgc gctcccgcac cggttaggcg
     1201 gcggctgctt ccgcagaccc ctccagcttc ttgtgctcga tctgggtgat cgtgccggcc
     1261 gtgatctcga tcttgcgcgg cttcagttcc tctggaagct cacgcttcag gtcgatgatc
     1321 agcaggccat cgcccatcgt ggcgccctcg acctcgatgt agtccgccag gtcgaagtga
     1381 cgctcgaagc tgcgggccgc gatgccgcga tgcaggtatg tgcgttcggt cctgtccttg
     1441 agctgaccgc gaaccgtcag ccggttgcgt tctgcgatga cggaaatgtc atccttgctg
     1501 aatccagcca cggccatgac gatgcggtac ttgtcctcgc cacacttctc gatgttgtag
     1561 ggaggataac cgctctcttc gcgctgcatg gcatggctga gcagcatcga gacgcggtcg
     1621 aaaccgaccg tggatcggta gaggggagtg aagtccagtg ttgccatagc catcctcctt
     1681 tcgaagcgac aaccacggaa atcacgggaa cgggctccca aatgggctgc ccgataagcg
     1741 atctaggtcg cgatgccctg gattcaagag gtgtacggaa tacgttggtg cgcagtccag
     1801 tcctgcgcgc tggcctcagt cggtcaggcc gtggcaagct gccgcatgcc gtcagcccat
     1861 tcgagccatg tgcgaatgcg cgcttccggg tccggatggg tgaagaagtc ggtgatgacg
     1921 gaggcagact gggcacccgc ttcgatgacg ctcgaggccc gttccggcgt gatgccgcca
     1981 atggccacga gcggcagtgc gccaatgcgc gccttccact cgcggatgcg gtcgagcccc
     2041 tggggggcgt acctcatgac cttgagcttc gtttcccaga tgggaccgag agctacatag
     2101 tcgggctcgg ctgccagcgc agtctcgagc tcctcggggg tgtgggtaga aatgccgatc
     2161 ttgatcttat gtgccttgag cgtcggcacg tcggcgccgg ccaagtcctc ctggccgagg
     2221 tggacgtagt cggcgcccgc gtctatcgcc tcgcgccagt agtcgttcac gatcagctgg
     2281 cagccgtggc gggtgcagag ttcgaggcct tcgaggatct gccgccggat ctcgtccggg
     2341 ctcgcgttct tgatgcgaag ctgtacggtt cgcaccccga gcgggacgat gcgcttcaac
     2401 cagccgacgt ccggtacgat cggatagaaa acgtcgagtt tgccggctgc gtcagcggca
     2461 gaagcggtca tcgcctcccc ccgaaatcga aaaacggcgt gccggccacc ggggtggacg
     2521 gctcggccat gtcgcgcact tccatcaggc ccgcttcgaa agccatccgg ccggcctcga
     2581 tggctgcagc aaaggccttc gccatcagga cgggatcgcc ggccttcgag acggccgtgt
     2641 tgagcagcac tgcgtcgtac cccatctcca tggcctcgct cgcgtgcgag ggggcgccga
     2701 tgccggcgtc gatgatcagc ggcacgccag ggaagtaatt gcgcagcgtt ttcaatgcgt
     2761 acttgttgtt gagcccgcga ccggtgccga tgggcgcccc ccagggcatc agaacctcgc
     2821 agccggcggc catcaagcgc tcggcggcgc cgaggtcctc ggtcgtgtac gggaaaatct
     2881 tgaacccgtc ctcggcaaga acgcgcgctg cttcgaccag gccgaacagg tccggctgta
     2941 gcgtgtcgtc gttggcgatg acctccagct tcacccaatc ggtctcgaac agctcgcgcg
     3001 ccatctgcgc ggtggtgatg gcctccttag gcgtccggca gccggccgtg ttgggcagca
     3061 cgcgcacgcc aagctcgcgg atcagctccc agaaccgctg cccggtcctg ccgccggcag
     3121 tctcgcgcct gacggaaacc gtcacgatct cagtgcgcga cgccttcacc gcgtccgcca
     3181 ggatctgcgg cgacgggtaa tgtgccgtgc cgagcagcag tcgcgagccg atgctggttc
     3241 cgtagacctt cagcggcgcg gccgcacgtg actgttggat gtcgatagcg ttcatctgtc
     3301 ctcagccgcc ctgacgcggc gatacgatct cgatccggtc cccatgcgca agctgccggc
     3361 cggccctcgc gcgctcgggc acgaattccc cgttgaccgc ggttgcaata cgtgtgtcgg
     3421 caaatccggc ctcgatcacg agctcggcaa gcgtgcgtgc ttcagttgcg agtggctcgc
     3481 cgttgacgaa gacgttcagg cgcgaaccgg catcggactg caaatccatg atggtcacaa
     3541 agattctacc ggatacccga cacatcgccc cgaagggcgc cgattgcaag ggccgcttgc
     3601 ctcattgtcg cttgtcctat tgaagtgcga tgcgtcccga agcgcaagtc cgccaagagc
     3661 gcatctgtat tgtctcccga gccgttccgg ccgatgttga cggcttcacc aagggaggat
     3721 tcgaccatga gtctcgatcg cttcagcctg aagggccggt gcgcgctcat caccggatca
     3781 agccagggta tcgggtatgc gattgcgaat ggtcttgcca cagccggtgc gacggtggtc
     3841 ctgaacgggc gggatcaggc gaagctgcag aaggcgctca ctgccgtaca atccgccggc
     3901 cataccgctc atgcgcgggc attcgatgtg accgactccg aggcggcaac cgctgccgtc
     3961 gaggccatcg agcgggaaat cgggccgatc gatattctcg tcaacaacgc cggcacgacc
     4021 cgccgcatgc caacggaaga tctgcccgac gagatctggc acgagatcat cagaacgaac
     4081 ctcgacagcg tcttctacat gtcccgcgcc gtcggccggc acatgatcgg gcgcaagcgc
     4141 ggctcgatca tcaacatctg ctcggtcatg agcgagctgg gccggcccac catcgtcccc
     4201 tacaccgctt ccaaaggtgg cgtgaagatg atgacgaagg gcctcgctgc cgactggggc
     4261 aagcacggca tacgtgtcaa cggcatcgcc cccggctact tcaaaacgcc gctcaacgag
     4321 gcgctggtga aagacgagaa gttctcgtca tgggtggaga gcaggacacc gctcggccgc
     4381 tggggcgaag tggacgagct ggtcggcgcc gcggtcttcc tggcgtcaga tgcggcgagc
     4441 ttcgtgaccg gtcacgtgct gttcgttgat ggcggcatga cttcggtggt ctgacgcgat
     4501 ggtggtcagt gaccgccgat caggtgcggc aaggtcagtg aaaccgccgg gatgtaggtc
     4561 acgagcatca acacgatgat cagcgtcgcg aggaacggca gggaggcgcg cacgacggct
     4621 gcgggcgaca cgtcgccgat cgacgccatg aggtagatga gagcgccaac gggtggcgtg
     4681 cacagcccga tcaccagatt gagcacgatc atgacgccga agtggatcgg gtccatgccg
     4741 atcgcggtga ccatggggag gaacacgggg accagaatca ccagggcggg caagggatcg
     4801 agaaacatgc ccacaacgaa gaagatcacg ttgagcgcca gaagcagcac ccagggctcc
     4861 aggtcgaggc cacggatggt ggccgcaagc tcctgaccga ttctctggtc ggcaatgatc
     4921 cacgcgaaga tcgacgaggc acccaggatc acgagcacgg caccggtccc gtgtgccgcc
     4981 cggatcaacg catcgagagt gccgcggaaa gacaaggcgc ggtaggcaaa gacaccgatc
     5041 agcagcacgt agacggttgc aacggccccc gcctctgtcg gggtgaacac gccgccacgg
     5101 atgccgccga cgatgatcac gggcgccaag agcgccaggc ccgcacgcag cgtttcggac
     5161 aggatcacgg ttttagacac ccgctccgag cgcggcaggt tcatgcgcct ggcgctgatg
     5221 tagaccacgg tgctgaggcc gatggcgatg agcacgccgg gcaggatgcc ggctatgaac
     5281 atcgttccga cagaaacatt cgcgagcacg gcatagatga tcatcgggat cgacggcggg
     5341 atgatcggtc ccaaagtcga tgccgtctct acgagggccg ccgagaagct cgccgggtag
     5401 ccttccttct tcatggagcg gatcaggagc gggccgacgg cggccgcatc ggccaccgcg
     5461 gagcccgaca cgccggccat caccaccgag ctcacgacgc cgacctggcc caggccgcca
     5521 cgcatgtggc cgacaagtgc ccgggcgaac gtcaggatgc ggtcgatgac accgcttgcc
     5581 gtcatcagct cgccggcgag aaagaacagg gcgatcgcca gcagcacgaa gttatcgatg
     5641 ccgccgatga agagctgtgg caggactgtc agcggaagct caggccggat gatgaagagc
     5701 gtgaaaaccg aggcaccgag cagggagaac accaacggga ttcccagggc agtgagcgcg
     5761 agcatcacgc cgagaagcag ggtcagcgcc atgaactagt tttcccctcg tagcctgccc
     5821 gcgacacctg ccaccgtgaa caccagccag aggaccccac caatcaacat ggcccggtag
     5881 agccactgca ccgagaccgg caaggcggtg taccggtcat aggccgtcag ctcgatgagc
     5941 gtcagggcat gccaaatcat gaagcccaga aggctagccg cgacgagggt ttgcagaact
     6001 accagtgctt ggcgcaccct tggcgatacg gcttgctcca gcaggtcgat gcgcatatga
     6061 gccgtccgcg tcgcgccgag aagaacaagc caaacgaaaa gcagacgcag caactcctcg
     6121 ccccagggcg tggagacgcc gagcatgtag cgcgcgatca cctggtaaaa ggcgagtgcc
     6181 gcgatgactg cgagcaacgc ggcacatacg ccatcgacga ctcgctggag cagggccagc
     6241 atcggatctt cactgtcccg cagcgatgat cttgtcgacg atctcgctac cgtactcctt
     6301 cacgtaggtt tccttgaccg gctcgaacac agctttccgg aaaggctccg tatcgatctc
     6361 ttcgatcact tcgacgccgg ccttacggaa ctcctcgagc gccgtcttgt cggcctcgcg
     6421 attgagcttc cgctgcagtt gctgcgcttc cttggcggcc tgaagcagcg ccttccgatg
     6481 ctcctcgctc aagctattga atttctgctg gttcatgacc gtgatggaca cggtgtacgc
     6541 atgccgcgtc atcgacaggt gcttctgcac ctcgtggaaa cggttcgagt gaatgatcgc
     6601 gagtggattt tcctgtccat cgaccgtacc ggactccagt gcgaggtaga cctcggtgaa
     6661 cggcatcggc gtcgggattg ctcccaagat cttgaaggcc tcgacgtggg caggatttgg
     6721 cgtcgtgcga atcttcaggc catcgagatc ctgcggcgtg cggatcgagc ggcggctgtt
     6781 tgtgatgttg cgaaagccga tctcccagta agccagcggc tgcatccggt gcttctggag
     6841 gccgtcgatc agctcttggc cgatcgggcc gtcgatgatc cgataggcat gttcatagct
     6901 cttgaagacg tagggtagat ccagcacgtt gagccgcggc tcgaagcggg tgaagaacgg
     6961 attgccggtg ttcgcgatat cgatggttcc gagccgaacg ccttcgagta ttgccggatc
     7021 gttgcccaac tcgcccgccg ggtgcaccga aaccttgacc tcgccattcg tga
//
LOCUS       DASVDU010000060        24948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1511472, whole genome shotgun sequence.
ACCESSION   DASVDU010000060 DASVDU010000000
VERSION     DASVDU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 24948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24948
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511472"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..301)
                     /gene="groES"
                     /locus_tag="VF226_04045"
     CDS             complement(11..301)
                     /gene="groES"
                     /locus_tag="VF226_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7073189.1"
                     /translation="MKFRPLHDRVVVRRVESETKSAGGIIIPDTAAEKPQQGEVIAVG
                     PGARDESGKVVALDVKVGDKVLFGKWSGTEVKIDGQELLIMKESDIMGVLDR"
     gene            568..2166
                     /locus_tag="VF226_04050"
     CDS             568..2166
                     /locus_tag="VF226_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073190.1"
                     /translation="MLTSAKLLASALLAIALLAGTADARTVKWARSGDSLTLDPHAQN
                     EGVTHALAHHIYEPLIIRDYQGKPLPALAESWELTSDPTVWEFKLRRGVKFHDGTPFT
                     VDDVIFSYERAMQPTSDMKSLLSSVDKLIRVDDYTLQIKTKGPNPLLPANLTDLFIMS
                     KAWAEKNNAVKVQDFKNKEENYAVRHANGTGPFVLVSREPDVKTVMKRNENYWGKSQM
                     PAEITELVYLPIKSDATRVAALLSGEVDFVQDVPVQDIQRLQSDENLRVVVGPENRAI
                     FLGLNVGDPELKTSNIKGRNPFADKRVRHAINMAINRQAIQRVVMRGQSIPTGIIAPP
                     GVNGYTKELDVIPPNDVEKAKQMLAEAGYPDGFSVTLHCPNDRYVNDEAICQAVVGQL
                     AQIGIQVNLVSQSKSLHFPIVQKDEADFYLLGWGVPTFDSQYIFSYLYHTKTDKEGGW
                     NGTRYSNPKIDEMTASLSRETDLVKRNQTIAEMWKILQDETIYIPIHIQTLAYAMKPD
                     LDIPVDISNQPKLKFLKIKKQQNQ"
     gene            2211..3194
                     /locus_tag="VF226_04055"
     CDS             2211..3194
                     /locus_tag="VF226_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073191.1"
                     /translation="MIAYALRSVAQALLVLLAVALIAFSMFRFVGDPVANMMGQEASQ
                     ADRRALAEQLGLNDPILVQFGRFIGDVAQGDFGISYRHGRKVSDLILERLPATVELAA
                     LSAILATVVGIVLGVYTAIRRGGLLAHIIMTTSLVGVSLPTFLIGIGLIYLFAVELRW
                     LPSFGRGETVKIGWWTTGLLTPSGRASLIMPVVTLAFYQLTLIMRLVRAQMLETLRTD
                     YVKFARARGLPESRIHFKHALRNTLIPVVTIAGLQFGSVVAFAIVTESVFQWPGLGAL
                     FVQAVQFVDIPVMATYLLFVALVFVVVNLIVDLLYVVLDPRLRAGRIRPVG"
     gene            3221..4162
                     /locus_tag="VF226_04060"
     CDS             3221..4162
                     /locus_tag="VF226_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073192.1"
                     /translation="MPVTRSRSFRSSLYRAWDSDLAWNFRHSPVAILASITTVSIILL
                     AVLAPWVAPQDAFDPGAVRLEDGFLEPMDRSPATGMLYVLGTDSQGRDLLSAILYGSR
                     VSLLVGFAAVGFSMVLGVMLGLVAGFIGGRTDAVIMRIADVQLSFPAILIALLVFGVG
                     RGLIPPSQHEEMAIAVLIVAIGLSHWAQYARTVRGSTMVEKGKEYVQAARVMGRPSFA
                     IMREHVLPNVLGPVLVIATINLALAIIEEATLSYLGAGVPPTQPSLGTLIRVGQQFLF
                     SGEWWILFFPSMALIVLALAINLLGDWLRDALNPRLK"
     gene            4201..5181
                     /locus_tag="VF226_04065"
     CDS             4201..5181
                     /locus_tag="VF226_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073193.1"
                     /translation="MPVPVLSVRKLNVAFATRRADLVALEDVSFDIARGEILGVVGES
                     GAGKSVTGAAIIGLIEPPGRITSGEIYLQGERIDTLPLGAMRKIRGKRIGMVFQDPLT
                     SLNPLFRIGEQIVETMTVHLDISPARARERAIELLGEVGIPAPEQRIDAYPHELSGGM
                     RQRVVLAMVLCTDPELIIADEPTTALDVSVQAQIITLLKNLAKSHGTSVMLITHDMGV
                     VAEMADRVAVMYAGRIVEIGPVRDVLKQPRHPYTQGLMGSIPTLTSDADRLVQIPGTM
                     PRLDARPPGCAFHPRCPRAIDRCRSERPLLTGDDRAMVACWLADSREAVS"
     gene            5178..6176
                     /locus_tag="VF226_04070"
     CDS             5178..6176
                     /locus_tag="VF226_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7073194.1"
                     /translation="MSQAPLVEVDRLWRVFDVSGPWLNRVLEGRPRQTLTAVADVSFA
                     IGRRETFALVGESGSGKSTIARIIVGLLPPSSGDVRIDGVSMSSTEARSKRAELRRRI
                     QMIFQDPYASLNPCWRVDKIIAEPLRAFGLYRVSQDIDGRVAELLELVGLDPADGQKY
                     PHEFSGGQRQRVAIARALAANPEFIVCDEPTSALDVSVQAQILNLMRDLQDRLGLTYL
                     FISHNLAVVRHMATRIGVLYLGRLVEVAPSHELFANPRHPYTRMLLDAVPDLEMTGRV
                     RRAVEGEIPNPINPPKGCGFHPRCPFQWERCRREAPELVTTGDRLVACHGVAEGRI"
     gene            6263..6559
                     /locus_tag="VF226_04075"
     CDS             6263..6559
                     /locus_tag="VF226_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073195.1"
                     /translation="MANPDEHRSRRLLSTAVPVLVALGVMAGDAIAQEETPLEILAVR
                     VREQGFPCDRPIKAERDQSASRPLSTVWFLDCGNARYRVVLHPDMGAEIQLQNR"
     gene            complement(6568..7755)
                     /locus_tag="VF226_04080"
     CDS             complement(6568..7755)
                     /locus_tag="VF226_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX7073196.1"
                     /translation="MSVAAGREFLSIPGPTTVPDEVLRAMHRPAVDIYSGGLIDVTMS
                     LLADLKRVFRTSGRTYMYAANGHGAWEAALCNVLSRGDKVLVLESGRFAVGWGEMGQK
                     LGVDVEVLKGDWRRAVRPSDVEERLRADKAHAIKAVLVVQVDTASGVVNDIPAISKAI
                     RAADHPALFMVDAVASLGTMPFEMDAWGIDVAVSGSQKGLMTPPGLGLVAANERAFAR
                     SRTADLNTPYWDWGFREGEEHYQKYCGTPPEHLVFALRQALDMLFAEGFEAAFERHRL
                     LAEAVRRAVSVWAEGQALAFNIADPHERANSVTTILWHRDGGPQPLIDYAREKCGVVL
                     GVGIGALSGKAFRIAHMGHVNAPMVLGTLGVIETGLSALGIPHGRGGVQAAIAWLGEN
                     VRP"
     gene            complement(7830..9158)
                     /locus_tag="VF226_04085"
     CDS             complement(7830..9158)
                     /locus_tag="VF226_04085"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006209480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7073197.1"
                     /translation="MTDTFDLVLKGGTVVNHDGTGVRDVGIRSGRIAALGSIDANAAG
                     EVIDCTGLHILPGVIDTQVHFREPGLEQKEDLETGSRAAVLGGVTAVFEMPNTRPLTT
                     SADALADKIRRARHRMFCDFAFYIGATRENVDDIPELERLEGSAGIKVFMGASTGELL
                     VEDEPTLDRIIARIGRRAAFHAEDEARLRERAGLRRPGDASSHPEWRDEIAALTATKR
                     LVRLAEKHGRRVHVLHVSTAEEMAFLADHKEWASVEVTPHHLTLVAPDCYQRLGTFAQ
                     MNPPVRDERHRQAIWAALSAGIVDVLGSDHAPHTREEKEHPYPHSHSGMTGVQTLVPI
                     MLDHVNAGRLSLERLVDLTSHGPQRLFGIRNKGRIAVGYDGDLTIVDLKRQQTIDDSM
                     IASRCGWTPYHGVTVKGWPVGTIVRGRHVMWEGEVLGAAGGEPVRFYEAP"
     gene            9274..10158
                     /locus_tag="VF226_04090"
     CDS             9274..10158
                     /locus_tag="VF226_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEX7073198.1"
                     /translation="MTSMNIAPLHDRGVVRVSGADAAKLLQGLITADLDLLQKQPAAF
                     AGLLTPQGKILFDFFIVRREQEFLLETRREKAPDLAKRLTFYKLRANVTIADASAEHA
                     VAALWGDGKPELGSLAVVYPDPRLAEMGWRAIAPADFRWDQLGSTAATEGDYHAHRIT
                     LGLPEGGEDYVLGDTFPHEALFDQVGGVSFQKGCYVGQEVVSRMEHRSTARKRVVPVD
                     GAEPLQAGSDVKAGEAVIGKVGSVSGTRGLALLRLDRVGEALGKGHPITAGGVPVTVN
                     LPSWVRFGIPGREESAKV"
     gene            10160..10780
                     /locus_tag="VF226_04095"
     CDS             10160..10780
                     /locus_tag="VF226_04095"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7073199.1"
                     /translation="MAAVEQSTTGVVRCPWAGTDPLYARYHDEEWGVPKADDRALFEK
                     LVLEGFQSGLSWITILRKRENFRRAFDGFDPEKIARYGAKDIERLLADPGIVRNRLKV
                     EATIENARAYLKLAERQSFASFLWAFLPDGPIINRRGGMDKVPATTEHSLRISKALKK
                     EGFRFVGPTTVYAFMQSVGMVNDHLTSCHRHGPCAELQRAFKLPRS"
     gene            10777..11418
                     /locus_tag="VF226_04100"
     CDS             10777..11418
                     /locus_tag="VF226_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HEX7073200.1"
                     /translation="MSSPRRQSRAVSRSGEQPRAWQRMLSGRRLDLLDPSPLDIEIED
                     IAHGLARVARWNGQTLGTHAFSVAQHCLMVEEIFGALNPACPRSHRLMALLHDAPEYV
                     VGDLISPFKAAIGLDYKAFEMRLLRAIHTRFGLPPESSPDTTAAIKNADRIAAYFEAT
                     RLAGFSEDEGELYFGNPTGLPRRLADRLSRLEAWPTEEAQSRFLVRFRELVRP"
     gene            complement(11595..12935)
                     /gene="hemN"
                     /locus_tag="VF226_04105"
     CDS             complement(11595..12935)
                     /gene="hemN"
                     /locus_tag="VF226_04105"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003524841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX7073201.1"
                     /translation="MNPEVLERYAAPVPRYTSYPTAPHFTSAVNAEIYAGWLRELTPG
                     AALSIYVHIPFCNKLCWYCGCHTKATQQYRPVADYMEALLKEVSTVAGLLPPDCRTVH
                     MHWGGGSPSILSPDDIRRLADALSRSFNRNADGEFAVEVDPRHVDPERVAAFAAIGVN
                     RVSVGVQDFNPEVQKAINREQSVEMTRSVVEMFRAVGVRSINIDLVYGLPHQTRASVE
                     RTIEEVIALAPDRIALFGYAHLPSRLPHQRLIDDDALPDITERFAQSNRAANRLIAAG
                     YVRVGLDHFARPEDTLARGPLHRNFQGYTTDAADALLGLGASSIGKLPQGYVQNSAIT
                     PDYQRRVRNGGLATVRGRALTEDDRARSFVIERLMCDLRFPAEELRERFPEQAFPLIE
                     EANDLLRSDEDGLVEPEGACGFRVTEKGRLFLRTICACFDAYLGEGQAQHSVGV"
     gene            13227..14315
                     /gene="queA"
                     /locus_tag="VF226_04110"
     CDS             13227..14315
                     /gene="queA"
                     /locus_tag="VF226_04110"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEX7073202.1"
                     /translation="MRTDLFDFDLPEDRIALYPLPERDAARLLVIHPGAEPELADRSV
                     ADLPDLLRPGDVVVFNDTRVIPAALTGFRIRGGAESRVEFNLHKRIDESRWRAFARPA
                     KRLEVGDLVRFGDEGRVCLLGTLDATVAAKGEGGEVELAFSKHSAYLDEAIDALGDAP
                     LPPYIAGRRPVTEQDRTAYQTVFARREGAVAAPTAGLHFTERLLERLDERGIGRQFVT
                     LHVGAGTFLPVKAEDTREHRMHAEWGEITAETAKALNAARRNGGRIVAVGSTALRTLE
                     AAAAENGEIQPFAGETALFITPGYRFRAVDIMMTNFHLPRSTLFMLVSAFSGLDRMKR
                     AYAHAVEAGYRFYSYGDACLLYPAADQQ"
     gene            14312..15451
                     /gene="tgt"
                     /locus_tag="VF226_04115"
     CDS             14312..15451
                     /gene="tgt"
                     /locus_tag="VF226_04115"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7073203.1"
                     /translation="MTIGFGYRLIAQDAGARLGEITTPRGIIRTPAFMPVGTAASVKA
                     LYPEQVRQAGADIILGNTYHLMLRPGAERIARLGGLHAFMRWPWPILTDSGGFQIMSL
                     AGLRKITEEGVAFRSHVDGARHMLTPERSIEIQCLLGSDIQMQLDECIALPASVDEAR
                     RAMELSLRWAERCRRAFAEKSAPGQALFGIVQGGIYPDLRRRSAEELVELDLPGYAVG
                     GLAVGEGQAAMLKTLERTTPHLPAGKPRYLMGVGTPADIIESVARGIDMFDCVMPTRA
                     GRHGLAFTWNGRVNLRNARHAEDTRPLDERSSCPASRDYSRAYLHHLVKAGEYLGSML
                     LTWNNVWFYQELMAAIRKSIAEGRFAQFAAEHRERLNALEAENSE"
     gene            complement(15458..16432)
                     /locus_tag="VF226_04120"
     CDS             complement(15458..16432)
                     /locus_tag="VF226_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7073204.1"
                     /translation="MRLATFNLESLDLPPKSKVPIEVRAEALRPALARLDADVLCLQE
                     VNGQHVPDRPERVLAALDHVLEGTPYADYHRVSTSGPDGIGVADVHNVVTLSRWPIRA
                     AREILHTLVPSPRYQLVTAVPPACGPEPITFDRPLLLVDVELPGGKLLTVINLHLRAP
                     LAAVIPGQKQSAFVWKSIGGWAEGYFLSAVRRAGQALELRLLLEELFASDPHRLIAVA
                     GDFNAEDHDTPLRIAIGAEEDTGNGALSPRSLVLLDRAIPADKCWSVLHHGRPQMLDH
                     ILVSRALYGQFREIEVHNETLGDELVGFGKGTRSAASYHAPIVAEFAL"
     gene            16595..17473
                     /locus_tag="VF226_04125"
     CDS             16595..17473
                     /locus_tag="VF226_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX7073205.1"
                     /translation="MREFAYERPDSIASAVQLMQKPDARPLAGGTDLIPQMREGRRTA
                     GLVIDLKGVGELTAIEDLPDGGLAIGAAASASAIARHARVLADYPAIASSVRLIGSVQ
                     VQSRASLGGNICNAAPSADGVPALICYRARARIAGPEGTREVEIEDVFAGPGRTTLKP
                     GELLAAIVLPPPQPRSAAHYHRFTPRREMDIAVAGAGTWISLDEQGRISDARIALASV
                     APTPLRAQAAKKRLIGEAPSRALLEEAGRLASADVRPISDTRGSADYRRTLVSVLTAR
                     ALAVCCRSLGVEVDVP"
     gene            17470..17943
                     /locus_tag="VF226_04130"
     CDS             17470..17943
                     /locus_tag="VF226_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7073206.1"
                     /translation="MKKLIQCTVNGDEHAVLADPRDTLLELLRDRLELTGTKEGCSNG
                     NCGTCTVLVDGEPVNACLMLAVEAPGHDIVTIEGISRGAELNPIQQALVDHGGTQCGF
                     CTPGIVMSAKALLDAKPRPTEAEIRHAIAGNLCRCTGYDKIVRAIAAAAAVVAGE"
     gene            17948..20233
                     /locus_tag="VF226_04135"
     CDS             17948..20233
                     /locus_tag="VF226_04135"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7073207.1"
                     /translation="MTTSSPVEDKRELRVVGRRLPRVDARERVTGKAVYPADLHLRDL
                     AHAKLLRSPYAHARIRGIDTSKAEALKGVLAVATAADFVDLPFGASVTMGEGSFDVWI
                     VNQANMAREKVFWVGQPVAAVAAVDVHTAEEALKLIEVDYEPLPPVVDLAEAMKPDAQ
                     VLHSHVVTKGVEPAPRSPSNVCSLTTISRGDTEAAFAEAAATGEIQVKVDTAHQGYLE
                     PPVVVAQVDPNGFATVWASTQGQFTAELMLARILGLPQSKIRVVPLEVGGAFGGKIAL
                     HGEAVAVKLSEKCGRPVRLMLSREEVLQGGTGPAAGAMIDIRVAADKDGRLTAIKGTY
                     RVDAGGLPGMSPSLLMQASAALYQCPNLRLEGYDVVTNKPRTEAYRGPGGIQAAFAME
                     QAMDILCQRLDMDPLEFRKRNASVTGSLMPIGTPFPPIGLTTILEEVGRHPCWTEPLQ
                     PGKHPRGRGLALGYWRGTSMTSAAHVTIAGDGRPMVTMGPVDISGTRTAMAQVVAEEF
                     GLSIDDVHVSTGDSKSVGYSDVAAGSRVGRTTTAALVEACADALRQLRSRAAEKLQSR
                     PEDVAYANGVFRAPGGAAISLSELMAATLGEGAIVGRGVSTRLPLGVEIGAHVCDVEV
                     DPGTGQVTVLRYTAFQDVGRALNPPAVEGQMQGSVVQGLGWALTEGFDYGPDGRLRNA
                     SLLDYRIPTALDVPPIDCVIIETPVPNVPYGVRGVGEVPIVPVAGAVANAVARAIGVR
                     VLQMPMTPERVLNAMHAGMPS"
     gene            complement(20253..20912)
                     /locus_tag="VF226_04140"
     CDS             complement(20253..20912)
                     /locus_tag="VF226_04140"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7073208.1"
                     /translation="MALQINSIAPDFEADTTEGRIRFHEWLGDSWCLFFSHPKDFTPV
                     CTTELGSLAKIKPEFDRRGVKLVGLSVDPVENHKLWSKDIEETQGVAPNYPMIGDPEL
                     KVSKLYSMLPADAGNTSEGRTPNDNATVRNVFVIGPDKKIKLILTYPMSTGRNWDEVL
                     RAIDSLQLTAKHNVSTPANWKPGDDVIISGSVSDDEARKKYPQGWKAPKPYIRIVSQP
                     N"
     gene            21549..21956
                     /locus_tag="VF226_04145"
     CDS             21549..21956
                     /locus_tag="VF226_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7073209.1"
                     /translation="MKLKLHHVNLTTSNVPAMTEFYRDVLQLEEDPQLDATRVTSQGY
                     AGKVGFVTDGNMQLHLAERDLNCAFRTGHAINPLERGHIAFRTDDIEAFKKRLKEKGI
                     PFSDYGAWAMNGWEQIFFHDPDGNIIEVHQVKT"
     gene            22358..24484
                     /locus_tag="VF226_04150"
     CDS             22358..24484
                     /locus_tag="VF226_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7073210.1"
                     /translation="MDKKPVLPRDIVDHIAAFVGVLDLDGTVAQVNETTLKISGSQRH
                     ELIGTKLWDLSWFTSDPESAARVKDAVTRAAKGETVRFDTTVTTHGGERLAIDLMIHP
                     VLDEKTGKVQYLVPSGINITRRKAIEAELKRSRDLLERAQRVGHLGHWSFDLATKKLE
                     WSAEARRIFFGEEESREVSAETIEKLIHPDDLERVRAGMMAAIQAGESFATSYRIRRG
                     DGEERIIELQADPDYAPDGELLRVFGIVRDVTQAHLREQQLIEQQQLIDLSLEPILCW
                     SLDDGLLHWNPGCQHLYGYSREEALGHRIDELLHSQFPVPIAKIRKRLEAGESWTGEV
                     IQTAKDGRKIIVEARIDPVVSGERRIVMEAHRDITARRQAEQALKLSEERLAIATKLA
                     GVGFFDHDQIADVICFSPEPWAGMLPTEATLDDVFAIVHPDDLAPFREAIAKAHDPRG
                     TGQLEHEYRMIRKTGEIRWISVKSRTFFEGEGKERRPVRTVGAVVDVTEQRMWAEQQR
                     LLMGELNHRVRNTLSVVQAIATQTLRTAKDPQAFVQDFKGRLQAIASAHKLLNETTWQ
                     GANLTGLVREQLASSCGSGQVYTDGPEVWLPPQVALNLGLVLYELATNARKHGALSVA
                     NGRVQLSWQVVREDDKRVLRLTWQECGGPAVRPPISRGFGMGLIERTMGSAADGAARI
                     AFEPGGLRCVIDIALQSAERFAISAP"
     gene            complement(24554..>24948)
                     /gene="nuoB"
                     /locus_tag="VF226_04155"
     CDS             complement(24554..>24948)
                     /gene="nuoB"
                     /locus_tag="VF226_04155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX7073211.1"
                     /translation="IRQVDAGSCNGCELEIHALNNIYYDLERFGLRFVASPRHADVLL
                     VTGPVTKNMREALERTYNATPDPKWVVAVGDCALNGGLFAGSYACAGGVSDVIPVDLH
                     IRGCPPSPTDMLKGLIALVESKAVAGPA"
BASE COUNT         4421 a         7956 c         8109 g         4462 t
ORIGIN      
        1 gctggatcgc tcagcgatcc agaaccccca tgatgtcgct ttccttcatg atgagcagct
       61 cctgcccatc gatcttgacc tcggtgccgg accatttgcc gaacagcacc ttgtcgccga
      121 ccttgacgtc gagcgcgacg accttgccgg actcgtcacg ggctcccgga ccgacagcga
      181 tcacttcgcc ctgctggggc ttctcggcag ccgtatcggg aatgatgata ccgccagccg
      241 acttggtttc gctctcgaca cggcgcacga cgacacggtc gtggagggga cgaaatttca
      301 tggtttcagc gctcccgcga ttgggatcta tgtggtttgg agtgtttgga agcacgttag
      361 cactcattcg aggcgagtgc taacacggac gtgtgatacg ggcgcccacg cgtaatgtca
      421 acagcggttc gcggcagctg gaaagcagct gctggcgcag ggctcaattg agccagggcg
      481 caggccctgg cggtgacatc ccggggtatg ccggctatga tcgcggctcc tgccggccac
      541 attacgatcg ttggaggaga caagggtatg ttgacgagtg ccaagctgct cgcgtccgcg
      601 ctgctcgcaa tcgcattgct ggccggaaca gccgatgcgc gaacagtgaa gtgggcacgc
      661 tccggcgact ctctcactct tgatccccac gcccagaatg aaggcgtaac gcacgctctg
      721 gcccaccaca tctacgagcc gctgatcatc cgcgactacc agggcaagcc gctgccggcg
      781 ctcgcggagt cctgggagct cacctccgat ccgactgtct gggagttcaa gctgcgccgc
      841 ggcgtcaagt ttcacgacgg caccccgttc acggtcgacg acgtcatctt ctcctacgag
      901 cgcgcgatgc agccgacctc cgacatgaag tcgttgctgt cgtcggtcga taagctgatc
      961 cgcgtcgatg actacacgct gcagatcaag accaaagggc ccaatccgct tcttcccgcc
     1021 aacctgaccg acctcttcat catgagcaag gcgtgggccg agaagaacaa cgccgtgaag
     1081 gtgcaggatt tcaagaacaa ggaggagaac tacgccgtcc ggcatgccaa cggcaccggg
     1141 cctttcgtcc tcgtcagccg cgagcccgac gtgaagacgg tgatgaagcg gaacgagaac
     1201 tactggggta agagccagat gccggcggag atcaccgagc tcgtctatct ccccatcaag
     1261 tcggacgcga cgcgggttgc ggcccttctg tcgggcgagg tcgacttcgt gcaggacgtg
     1321 ccggtgcagg acatccagcg cctgcagagt gacgagaacc ttcgcgtcgt cgtcggcccg
     1381 gagaaccggg ccatcttcct gggactgaat gtcggcgatc ccgagctcaa gacatccaac
     1441 atcaagggca ggaacccgtt cgccgacaag cgtgtccgcc acgccatcaa catggcgatc
     1501 aaccggcagg ccattcagcg cgtcgtcatg cgcggccagt cgatcccgac ggggatcatc
     1561 gcgccgccgg gcgtcaacgg ctacacgaag gagctcgacg tcattccgcc gaacgatgtc
     1621 gagaaagcaa agcagatgct cgcggaggcc ggctatccgg acggcttctc ggtcacgctc
     1681 cactgcccca acgaccgcta cgtaaacgac gaggcgatct gccaggccgt ggtcggtcag
     1741 ctggcccaga tcgggatcca ggtgaatctc gtttcacagt cgaaatcgct gcatttcccg
     1801 atcgtccaga aagatgaagc ggatttctat ctgcttggct ggggcgttcc gaccttcgat
     1861 agccagtaca tcttttcgta cctctaccac accaagaccg acaaggaggg ggggtggaac
     1921 ggcacccgct actccaatcc aaagatcgac gagatgacgg ccagtctcag ccgggagacg
     1981 gacctcgtaa agcgaaatca gaccatcgcc gaaatgtgga agatcctgca ggatgagacg
     2041 atctacatcc cgatccacat ccagacgctg gcctatgcga tgaagcccga cctcgacatt
     2101 ccggtcgaca tctccaacca gccgaagctg aagtttttga agatcaagaa gcagcagaat
     2161 cagtagcaag cacgggccgg cccttgccgg cccgtctcac gcgggccgca atgatagcct
     2221 atgccctgag gagcgtcgcg caggctctgc tggtgcttct cgccgtcgcc ttgatcgcct
     2281 tctcgatgtt ccgcttcgtc ggtgatccgg tcgccaacat gatggggcag gaggcgagcc
     2341 aggcagatcg ccgcgcgctt gccgagcagc tcggcctcaa cgatccgatc ctcgtgcagt
     2401 tcggccgctt catcggggat gtggctcagg gcgacttcgg catctcctat cgccacggcc
     2461 gcaaggtctc cgatctcatc ctggagcgct tgccggccac cgtggagctc gccgcattgt
     2521 cggcgatcct cgcgaccgtg gtcggcatcg ttctcggggt ctacacggcc atccggcgag
     2581 gcggcctgct ggcacacatc atcatgacca cgtcgctcgt cggggtgagc cttccgacct
     2641 ttctcatcgg gatcggactg atctacctgt tcgccgttga gctcagatgg ctgccgtctt
     2701 tcggacgcgg cgaaacggtg aagatcggct ggtggacgac gggactgctg accccgtcgg
     2761 gccgagcctc tctcatcatg ccggtcgtga cgctggcgtt ctaccagctc accctcatca
     2821 tgcgcctggt gcgggcccag atgctggaga cgctgcggac ggattacgtc aagttcgctc
     2881 gcgcgcgcgg gcttccggag agccgcatcc acttcaagca tgcgctgcgc aatacactca
     2941 ttccggtggt gaccatcgca gggctgcagt tcgggtcggt ggtcgccttt gccatcgtga
     3001 cggaaagcgt cttccagtgg ccggggctgg gggcgctgtt tgtccaggcg gtgcaattcg
     3061 tcgatattcc ggtgatggcc acttacttgc tgttcgttgc gttggtgttc gtggtcgtca
     3121 atctcatcgt cgacctgctc tacgtcgtgc tcgatcctcg gctgagggct ggccgtattc
     3181 ggccagtggg atagggggcg ccgtgcaaaa gcaccaacgg atgccagtta cccgatcgag
     3241 atctttccgc tcgtcgctct accgcgcatg ggattcggac cttgcgtgga atttccggca
     3301 ctcccccgtt gccattctcg ccagcatcac gacagtctcg atcatcctgc tcgcggtgct
     3361 ggcaccttgg gttgcgccgc aggacgcgtt cgatccgggc gccgttcgcc tggaggatgg
     3421 gttcctcgag ccgatggacc gcagccccgc caccgggatg ctgtatgtgc tcgggacgga
     3481 cagccagggc cgggacctgc tttccgccat cctctacggc agccgcgtct cgctgctggt
     3541 gggcttcgca gccgtggggt tctccatggt gctgggggtc atgctggggc ttgttgccgg
     3601 attcatcgga ggacggacgg acgccgtcat catgcgcatc gccgacgtcc agctgtcctt
     3661 tccggcaatc ctgatcgcgc tgctcgtctt cggcgtcggc cgcggcctca tcccgccatc
     3721 gcagcacgag gagatggcga ttgccgtact gatcgtcgcc atcggcctgt cgcactgggc
     3781 tcagtacgca cgtacggtcc gcggctcgac catggtcgag aagggcaagg aatacgtgca
     3841 ggctgcgcgc gtgatgggcc ggccatcctt cgcgatcatg cgcgagcatg tgctgcccaa
     3901 tgtcctcggc cccgtgctgg tgatcgccac gatcaatctg gcgttggcga tcatcgagga
     3961 ggcgacgctg tcctatctcg gcgccggagt gccaccgacc cagccgtcgc tcggcacctt
     4021 gatccgcgtc ggacagcagt tcctgttctc aggtgaatgg tggatcctgt tcttcccctc
     4081 gatggccctc atcgttctcg ccctcgccat caatctgctc ggcgactggc tgcgtgatgc
     4141 tttgaacccg aggctgaagt gatgggatcg tctgcaccgg agcgttccac gcgaccggac
     4201 atgccggtgc ccgtgctgtc cgtccgtaag ttgaacgtcg ccttcgccac ccggcgcgca
     4261 gacctcgttg cgctcgaaga cgtgtcgttc gacatagcca ggggtgagat tctgggcgtc
     4321 gtgggagagt cgggagcagg caaatcggta acaggcgccg ccatcatcgg attgatagag
     4381 ccgcctggcc gtatcacgtc cggcgagatt taccttcagg gcgagcgtat cgacaccctt
     4441 ccgctcggcg cgatgcgcaa gatccgcggc aagcggatcg gcatggtgtt ccaggacccg
     4501 ctgaccagcc tcaacccgct gttccggatc ggcgagcaga tcgtcgagac catgaccgtg
     4561 catcttgaca tttcgcccgc ccgagcgcgt gagcgcgcaa tcgagctgct gggcgaggtc
     4621 ggcattccag cccccgagca gcgcatcgac gcctatccac acgagctctc cggcggcatg
     4681 cggcagcggg tggtgctcgc catggtcctc tgcacggacc cagagctgat catcgccgac
     4741 gagccgacga cggcgctcga cgtgtccgtc caggcgcaga tcatcacgct cctcaaaaac
     4801 ctggcgaaaa gccacggcac gtccgtcatg ctcatcaccc acgacatggg cgtggtcgcc
     4861 gaaatggcgg atcgcgtggc ggtgatgtac gccggccgca tcgtcgagat cggtccggtc
     4921 cgcgacgtgc tgaaacagcc gcgccatccg tatacgcaag gactgatggg ctccatcccg
     4981 acgctgacga gcgatgccga tcgtctcgtg cagattccgg gaacgatgcc ccggctcgat
     5041 gcacggcctc cgggctgcgc tttccacccg cggtgcccgc gggccatcga tcgctgccgc
     5101 agtgagcgac cgctgctgac cggcgatgat cgcgcgatgg tggcctgctg gcttgccgat
     5161 tcccgcgagg cggtgtcatg agccaggcgc ctcttgtcga ggtcgaccgg ctttggcgcg
     5221 tattcgacgt ctcgggtccg tggctcaacc gtgtgctgga gggtcggcca cgtcaaactt
     5281 tgaccgcggt ggccgacgtc tccttcgcca tcggccgccg ggagaccttt gcgctcgtcg
     5341 gcgagtccgg ctcgggcaag tcgacgatcg ccaggatcat cgtcggtctg ctcccgccga
     5401 gcagcgggga cgtgcgcatc gacggcgtct cgatgagcag cacggaggcc cgcagcaaac
     5461 gcgccgagct gcgccgccgc attcagatga ttttccagga cccgtacgcc agcctcaacc
     5521 cgtgttggcg tgtggacaag atcatcgctg agccgctgcg cgcgttcggg ctataccgcg
     5581 tcagccagga tatcgacggg cgcgtggccg agctgctgga gctggtcggc ctcgatcccg
     5641 cggacggaca gaaatatccg cacgagttct ccggcggtca gcgccagcgc gtcgcgattg
     5701 cgcgcgcgct tgctgccaat ccggagttca tcgtctgcga cgagccgacc tccgccctcg
     5761 acgtttcggt tcaggcgcag atcctcaatc tgatgcgtga cctgcaggac cggctggggc
     5821 tcacgtatct cttcatcagc cacaatctcg ctgtcgtcag gcacatggca acgcggatcg
     5881 gcgtgctcta tctggggcgg ctcgtcgagg tggcgccgtc acacgagctc ttcgccaacc
     5941 cgcgccaccc ctacacgcgc atgctgctcg atgcagtgcc ggatctggag atgaccggcc
     6001 gcgtgcgccg tgctgtggaa ggtgaaatcc ccaatcctat caacccgccg aagggctgcg
     6061 gcttccatcc gcgctgccca ttccagtggg agcgctgccg ccgagaggcc ccggagctgg
     6121 tcacgacagg agatcgcctc gtggcctgtc acggtgttgc ggaggggcgc atttaaccgc
     6181 gtctgcaagt ggcctggaac ctcaggggcg cccctcatgt tcgccagagg ccgtagtccg
     6241 tgtgcggagg cttccataac ctatggccaa tccggacgaa catagatcgc gccggctcct
     6301 gtctaccgcc gtgccggtgc tggttgcgct tggcgtcatg gctggtgatg ccatcgcgca
     6361 ggaggaaact ccgctggaaa tccttgcggt gcgcgtgcgg gagcagggct ttccttgcga
     6421 taggcccatc aaggccgaac gggatcagtc cgcttcaaga ccgttgagca ccgtatggtt
     6481 cctcgattgc ggcaacgcgc gataccgcgt cgtgctgcat ccggacatgg gcgcggaaat
     6541 ccagcttcag aaccgatagc tcacgtatca gggccgcacg ttctccccca gccacgcgat
     6601 cgcggcctgc acgccgccgc gcccgtgcgg aatgccgagc gcggacaggc ccgtctcgat
     6661 caccccgagc gtgccgagca ccatcggcgc gttcacatga cccatgtgag cgatccggaa
     6721 cgccttgcct gacagcgcac cgatgccgac gccaagcaca acgccgcact tctcgcgcgc
     6781 atagtcgatc agcggctgcg gcccgccgtc ccgatgccag aggatggtgg tcaccgagtt
     6841 ggcgcgctcg tgcgggtcgg cgatgttgaa cgcgagcgcc tgtccttccg cccagaccga
     6901 gacggcccgc cggaccgctt cggcgagcaa cctgtgccgc tcgaaagccg cctcgaaccc
     6961 ttccgcgaag agcatgtcga gcgcctggcg cagcgcgaag acgaggtgct ccggcggcgt
     7021 gccgcaatac ttctgataat gctcctcgcc ttcgcggaag ccccagtccc agtacggcgt
     7081 gttgaggtcg gccgtcctgc ttcgcgcaaa ggcgcgctcg ttggccgcca ccagcccgag
     7141 ccccggcggc gtcatcaaac ccttctgtga gccggaaacg gcaacgtcga tgccccacgc
     7201 atccatttcg aaaggcattg tgccaagcga ggccacggca tcgaccatga acaaggcggg
     7261 atggtccgct gcccggatgg cctttgagat cgccggaatg tcgttgacga cgccggaggc
     7321 cgtatcgacc tgcacgacca ggacagcttt gatcgcgtgg gccttgtccg ctctcagccg
     7381 ttcctcgacg tcactgggcc gcaccgcgcg gcgccaatcg cctttcagca cctccacgtc
     7441 gacaccgagc ttctggccca tctcgcccca gccgacagcg aagcggccgc tttcgagcac
     7501 cagaaccttg tcgccgcgcg agagcacgtt gcagagcgcc gcctcccagg cgccatggcc
     7561 attggccgcg tacatgtacg tccgcccgct ggtgcggaag acccgcttga ggtcggcgag
     7621 cagactcatc gtcacgtcga tgagcccgcc cgagtagatg tcgacggccg gccggtgcat
     7681 ggcccgcaga acctcgtcgg gaaccgtcgt tggtccggga atgctcagga attctcggcc
     7741 cgcggcgaca ctcatgggac atgctcctcg tcagtcagtc gggggtgaac gtcttccggc
     7801 agcagaggcg gtgcgtcaag ccatgacaat cagggcgcct cgtagaaccg caccggctcg
     7861 ccgccggctg caccgagcac ctcgccctcc cacatgacgt gccgcccgcg aacgatggtt
     7921 ccgaccggcc agcctttgac ggtcacgccg tgatagggcg tccagccaca gcggctggcg
     7981 atcatcgagt cgtcgatcgt ctgctgccgc ttcaaatcga cgatggtgag gtcgccgtca
     8041 tagccgacgg cgattcgtcc cttgttgcgg atgccgaaca gccgctgagg cccatggctc
     8101 gtgagatcga cgagccgctc gagcgacagg cggcccgcgt tgacgtgatc gagcatgatc
     8161 ggcacgagcg tttgcacgcc ggtcatgccg gagtggctgt gcggataggg atgctccttc
     8221 tcctcccgcg tatgcggcgc atggtcggag cccagcacat cgacgatgcc ggccgacagc
     8281 gctgcccaga tcgcctgccg gtgccgctcg tcgcgcaccg gcgggttcat ctgggcgaag
     8341 gtcccgagcc tttggtagca atcgggcgcg accagcgtca ggtgatgcgg tgtcacctcg
     8401 acgctcgccc attccttgtg gtcggcgagg aacgccatct cctcggccgt cgagacgtga
     8461 agcacgtgaa cacgccgtcc atgcttctcc gcaaggcgaa cgagacgctt ggtagcggtc
     8521 agagcggcaa tctcgtcccg ccactcggga tgcgatgacg catctcccgg acggcggagg
     8581 ccagcgcgct cccggaggcg cgcctcgtcc tccgcgtgga acgccgcgcg gcggccgatc
     8641 ctggcgatga tgcggtcgag ggtcggctcg tcctccacca gcaactcgcc ggtcgaggcg
     8701 cccatgaaca ctttgatccc ggctgagcct tcgagccgct ccagctccgg gatgtcgtcc
     8761 acgttctcgc gggtggcgcc gatgtagaac gcgaagtcac agaacatgcg gtggcgcgcg
     8821 cggcggatct tgtcggcaag agcatcggcg ctggtcgtca gcggcctcgt gttgggcatc
     8881 tcgaatacgg cggtcactcc gcccagcacg gccgcgcggc tcccggtttc caggtcctcc
     8941 ttctgctcga ggcccggctc gcggaaatgg acctgcgtgt cgatgacgcc ggggaggatg
     9001 tgcagacccg tgcagtcgat cacctcaccg gcggcattgg cgtcgatgct gccgagcgcg
     9061 gcaatacggc cgctgcggat gccgacgtca cgcacgccag tgccgtcgtg gttgacgaca
     9121 gtgccgccct tcagcaccag gtcgaatgtg tcggtcatac cacgtgccct ccatccgctt
     9181 gcttcagtgt ggcgcgccgc ttacgtactg ctacgtgccc acgctgccag tgcttcattt
     9241 tgcgctcggg tgcaagggac cggggatttc acgatgacca gcatgaacat tgcaccgctc
     9301 cacgaccggg gcgtggtgcg cgtgtcgggt gccgacgcag ccaagttgct gcagggactc
     9361 attaccgccg acctggacct gcttcagaag cagccggcgg catttgccgg cctgctgacg
     9421 ccgcagggca agatcctgtt cgacttcttc atcgttcgga gggagcagga gttcctgctc
     9481 gaaacgcggc gcgagaaggc gcccgatctc gccaagcggc tcacgttcta caagctgcgg
     9541 gccaatgtga ccatcgccga tgcctccgcg gagcacgcgg tcgcggcgct gtggggtgat
     9601 ggaaagcccg agcttgggag cctggctgtg gtttatccgg acccgcggct cgcggaaatg
     9661 ggatggcggg cgatcgcacc ggccgacttc cgctgggatc agctggggag caccgctgca
     9721 acggagggcg actatcacgc tcaccggatc acgcttgggc tgccggaagg cggtgaggac
     9781 tacgtgctcg gcgatacgtt cccgcatgaa gccctgttcg accaggtggg cggcgtgagc
     9841 ttccagaagg gctgctatgt ggggcaggag gtcgtctcgc gcatggagca ccgctcgacg
     9901 gctcgcaagc gcgtggtgcc ggtcgacggc gcggagccgc tgcaggccgg cagcgacgtc
     9961 aaggccgggg aggctgtgat cggtaaggtc ggttccgtat cggggacccg cggcctcgcc
    10021 ctgttgcggc tcgaccgggt gggagaagcg ctgggcaaag gccacccgat tacggcaggc
    10081 ggcgtgcctg ttacggtaaa cctgccgtcc tgggtccgct tcggcattcc cggacgcgaa
    10141 gagagcgcga aggtgtagcg tggccgcagt cgagcaatcc accaccggcg ttgtacgctg
    10201 cccttgggcc ggaacagatc cgctttacgc ccgctatcat gatgaggaat ggggggtgcc
    10261 gaaggcggac gaccgggcgc tcttcgaaaa gctcgtgctc gaaggctttc agtccggcct
    10321 ttcgtggatc accatcctcc gcaagcgcga gaacttccgc cgcgccttcg acggattcga
    10381 tcccgagaag atcgcccgtt atggcgccaa agacattgaa cggctgctgg ccgacccagg
    10441 catcgtccgc aaccgtctta aggtcgaggc gacgatcgaa aacgcgcggg catatctcaa
    10501 actggccgag agacagtcgt ttgcatcatt cctctgggcc tttttgccgg acggacccat
    10561 tatcaatcgt cgtggcggca tggataaggt gcccgcgacg accgagcatt cgctccgcat
    10621 ttcgaaggca ctcaagaagg aaggctttcg tttcgtcggg ccgacgacgg tctacgcctt
    10681 catgcaatcg gttgggatgg tgaacgacca cctgacgagc tgtcatcgcc acggcccttg
    10741 cgccgagttg cagcgcgcgt tcaagttgcc acggtcatga gcagtccccg ccggcagtct
    10801 cgggccgttt cgaggtcggg cgagcagccg cgagcctggc agcgcatgct gtccggccgc
    10861 cggctcgacc tgctcgatcc atccccgctc gatatcgaga tcgaggacat tgcacacggg
    10921 ctggcgcgcg tcgcccgctg gaatggtcag acgctcggca cgcatgcctt ctccgtcgcc
    10981 cagcattgcc tcatggtgga agagatcttc ggtgcactga accccgcttg tccgcggagc
    11041 catcggctca tggcgcttct gcacgatgcg ccggagtacg tggtcggaga tctcatcagt
    11101 ccgttcaagg cggccatcgg gctcgattac aaggcgttcg aaatgcgcct tctacgggcc
    11161 atccacaccc ggttcggcct gccgccggag tcgtcaccgg atacgactgc cgcgatcaaa
    11221 aacgccgacc gcatcgccgc ctacttcgag gccacacggc ttgccggctt ctcagaggac
    11281 gaaggcgaac tgtatttcgg caacccgacg ggcctgccgc ggcgactggc cgatcggctc
    11341 agccgcctcg aggcctggcc gaccgaggag gcgcaatcgc ggtttctcgt ccggtttcga
    11401 gagctggtgc ggccgtaacc cgcgctcggc tcgccgcaaa cgccagccaa gctctcgcag
    11461 tggaagacgc gagtaaaaga aatcgcttcc atcagaagcg attgctggaa acctggcctt
    11521 cgggcgggcc ccttgtcctg ggggttgaag caagcaccgt tgctgcttgc tttggaaaag
    11581 caacgagaac taggctacac tccgaccgaa tgctgcgcct ggccttctcc gagataagcg
    11641 tcgaagcagg cgcagatggt gcgcaggaac aaccggccct tctcggtaac ccggaaacca
    11701 caggcgccct ctggctcgac gagcccgtcc tcgtcggacc ggagaagatc gtttgcctct
    11761 tcgatcagcg gaaatgcttg ctcgggaaac cgttcgcgca gttcctcggc gggaaaacgt
    11821 aggtcgcaca tcagccgctc gatgacgaag ctgcgcgcgc ggtcgtcctc cgtgagtgcc
    11881 cgccctcgaa ccgttgcaag tccgccgttg cgcacgcggc gctgatagtc gggcgttata
    11941 gcgctgttct gaacgtagcc ttgtggaagc ttgccgatcg aggaagcccc gagacccagc
    12001 agcgcgtcgg cggcgtccgt cgtgtagccc tggaaattgc gatgcagagg gccgcgcgcc
    12061 agcgtgtcct cgggccgggc gaaatggtcg aggccaacgc gcacgtagcc cgcggcaatg
    12121 agcctgttgg ccgcccggtt cgactgggcg aaacgctcgg tgatgtcggg cagcgcatcg
    12181 tcgtcgatca agcgctggtg cggcagccgc gagggcagat gcgcatagcc gaacagtgcg
    12241 atgcggtccg gcgcgagcgc gatcacctcc tcgatcgtgc gttccacgct ggcgcgcgtc
    12301 tgatgcggca ggccgtagac gagatcgatg ttgatgctgc gcacccccac ggcccggaac
    12361 atctcgacca ccgaccgggt catctcgacc gattgctcgc ggttgatcgc cttctgcacc
    12421 tcgggattga agtcctgcac gccgacgctc acgcgattga cgccgattgc ggcgaacgct
    12481 gcgacccgct ccgggtccac gtgccgcgga tcgacctcga cggcgaactc gccatcggcg
    12541 ttgcggttga agctgcggct cagcgcgtcg gccagacgcc ggatgtcgtc cggcgaaaga
    12601 atgctcggcg acccgccgcc ccagtgcata tggacggtgc ggcaatcggg cggcagcagg
    12661 ccggcaacgg tcgagacctc cttgaggagc gcttccatgt agtcggcgac gggccggtat
    12721 tgctgcgtcg ctttggtgtg gcaaccgcag taccagcaaa gcttgttgca gaacgggatg
    12781 tgtacgtaga tcgacagcgc ggcccctggc gtgagctcgc gcagccatcc ggcgtagatc
    12841 tcggcattca cggctgacgt gaagtgcggg gccgtgggat agctcgtata ccgcggcacg
    12901 ggcgccgcat aacgttccag aacctcggga ttcatctgtg ctctgccaag ctcgatgcct
    12961 agcgccttgc tggttggggg aacctattta cggcaacggc gaagcttatt gatcctggtc
    13021 aaggtcacgc ggggctcaac tgtcgcagcg agcgctcctc tcgcaaccgc gggttgccaa
    13081 tagagttagc gccggaccgc cagcgatccg ctactcagag ccaccggatg cggccaacag
    13141 agcgtacttc ctggggcgcg cccgattgcc actccccctg atgacggatc tcgattacct
    13201 tctcgaaaaa agccaggaag acgcagttgc gaaccgatct cttcgacttc gacctgccag
    13261 aggaccggat tgcgctttat ccgctgccgg agcgcgatgc cgcgcgcctg ctcgttatcc
    13321 acccaggcgc cgagcctgag cttgcggacc gctccgtagc cgatctgccg gacctgctgc
    13381 ggccggggga cgtggtggtc ttcaacgata cgcgtgtgat tccagcagcc ctcacgggtt
    13441 tccgcattcg aggcggcgcg gaaagccggg tcgagttcaa cctgcataag cgaattgacg
    13501 agagtcgctg gcgcgccttc gcgcggcctg ccaagcggct cgaggtcggc gatctcgtcc
    13561 gcttcggcga cgaggggcgg gtctgcctgc tggggacgct cgatgcgacc gtcgcggcga
    13621 agggcgaggg cggcgaggtg gagctcgcgt tcagcaagca cagcgcctat ctcgacgagg
    13681 ccatcgatgc gctgggggac gcaccgctgc ccccttacat cgccggccgc cggcctgtga
    13741 ccgaacagga tcgcacggcc taccagacgg tctttgcgcg ccgtgagggc gccgtcgcgg
    13801 cgccaactgc ggggctgcat ttcacggaac ggctgctgga gcggctcgat gagcggggga
    13861 tcgggcgaca gttcgtgacg cttcacgtcg gtgccggcac gttcctgccg gtgaaggcgg
    13921 aggacacgcg cgagcatcgc atgcacgccg agtggggcga gatcactgcc gagacggcca
    13981 aggcactcaa cgccgcccgc cgcaacggcg gcaggatcgt cgccgtcggc agcaccgcgc
    14041 tgcggacgct cgaggctgcg gccgccgaga atggcgagat acaacccttc gcaggcgaaa
    14101 cggcgctctt cataacgccg ggatatcggt tccgcgccgt cgatatcatg atgacgaact
    14161 ttcacctgcc gcggtcgaca ttgtttatgc tggtctcggc cttctcgggc cttgaccgca
    14221 tgaagcgcgc atacgcccat gccgtggagg ccgggtaccg cttctactcc tatggcgatg
    14281 cctgcctgct ctacccggcc gcagaccagc aatgacgatc ggcttcggtt accggcttat
    14341 cgctcaggat gccggtgcgc gcctcggtga gattacgacg ccgcgcggga tcatccgcac
    14401 gccggccttc atgccggtcg gcacggcggc gagcgtgaag gcgctttacc ctgagcaggt
    14461 gcggcaggcg ggagccgata tcatcctcgg caatacctat catctgatgc tgcggccggg
    14521 cgcggaacgg atcgcgcggc tcggcggctt gcacgcgttc atgcgctggc catggccgat
    14581 cctgaccgac tccggcgggt tccaaatcat gtcgctggca ggcctcagaa agatcaccga
    14641 ggaaggcgtg gcgttccgtt cgcacgtgga cggagcaagg cacatgctga ccccggagcg
    14701 gtccatcgaa atccagtgcc tgctcggcag cgacatccag atgcagctcg acgagtgcat
    14761 cgcgctgccg gcgagcgtcg acgaggcgcg ccgcgccatg gagctgtcgc tgcgctgggc
    14821 ggagcggtgc cggcgcgcgt tcgccgagaa gagcgctccc gggcaggcgc tgttcggcat
    14881 cgttcagggc gggatctatc ccgacctgcg ccgccggtcg gccgaggaac tcgttgagct
    14941 cgacctgcct ggctatgccg tcggcgggct cgcggtgggc gaaggccagg cggcgatgct
    15001 caagacgctg gagcggacga cgccgcatct gcctgcgggc aagccgcgct atctgatggg
    15061 cgtcggtacg ccggccgata tcatcgagtc ggttgcgcgc ggcatcgaca tgttcgactg
    15121 cgtgatgccg acgcgcgcag gccgccacgg gctcgcgttc acctggaatg gccgcgtgaa
    15181 cctgcgcaac gcacgccacg cggaggatac gcgcccattg gacgagcgga gcagctgccc
    15241 ggcttcgcgt gactactcgc gggcttacct gcatcatctg gtgaaggccg gcgaatatct
    15301 cggctccatg ctgctcacct ggaacaacgt ctggttctat caggagctga tggcagccat
    15361 tcgcaaatcc atcgcagagg gccgattcgc gcaattcgct gcggagcacc gcgagcggct
    15421 caatgcgctg gaggcggaga atagtgaata gccggcgtca gagcgcgaac tctgccacta
    15481 tgggcgcatg gtaggaggcg gccgagcggg tgcccttgcc gaagccaacc agctcgtccc
    15541 caagcgtttc gttgtgcacc tcgatttcac ggaattggcc gtagagcgcg cggctcacga
    15601 ggatgtggtc gagcatctgc gggcggccat ggtggagaac gctccagcat ttgtctgcgg
    15661 ggatggcgcg gtcgagcagc acgagcgagc gcgggctgag cgcaccattg cctgtatctt
    15721 cctccgcgcc gatcgcgatg cgaagcgggg tgtcgtggtc ctcggcattg aagtcccccg
    15781 cgaccgcgat gagccgatgc gggtccgacg cgaacagctc ttccagcagc agccgcagct
    15841 cgagggcctg gccggcgcga cgcaccgccg aaaggaaata accttccgcc catccgccaa
    15901 tgcttttcca cacgaatgcg ctttgcttct ggccgggaat gacagcagcc agcggggctc
    15961 tcaagtgcag gttgatgacg gtgaggagct tgccgccggg aagctcgacg tcgaccagca
    16021 gcaaagggcg atcgaacgtg atgggctccg gaccgcaggc aggtggcaca gccgtgacca
    16081 gctggtagcg cggagaaggc accagcgtgt gcaaaatttc ccgtgcggca cggatgggcc
    16141 agcgtgacag agtcacgacg ttgtgcacgt cggcaacgcc gataccgtcc ggaccgctgg
    16201 tcgagacacg gtgatagtcg gcgtagggtg tgccttcgag gacgtgatcg agggctgcca
    16261 gaacgcgctc cgggcggtcg gggacgtgct ggccgttcac ctcctgcagg cagaggacat
    16321 cggcatcgag gcgcgccaga gcagggcgca gagcctccgc gcgcacctcg atcggaacct
    16381 tcgatttcgg cggcagatcc aggctttcca ggttgaacgt cgccaatcgc atgaacggct
    16441 ccgatgctga ggctttgctt caagatgatg acattccaca cgcccattcc agaacccgga
    16501 tgcaggccgc tacggggccg atagatggac attcggcgcc gcttcggtct acttcaacgc
    16561 agcaacaagg caagcggctg cggagagcac gccattgaga gaattcgctt atgagcgtcc
    16621 ggacagcata gcgagcgctg tccaactgat gcaaaagccg gatgcgcgcc cgctcgccgg
    16681 cgggacggat ctcatccccc agatgcgcga gggacggcgg acggccgggc tggtgattga
    16741 tctgaaggga gtcggcgagc ttacggcaat cgaggatctg ccggatggcg ggctggcgat
    16801 cggcgcggct gcaagtgcct cagcgatcgc acgccatgcc cgcgttctcg ccgactatcc
    16861 cgccatcgcc agctcggtac gcctgatcgg aagcgtccag gttcagagcc gcgccagcct
    16921 cggcggaaat atctgcaatg cggcaccgtc cgcggacggt gttccggcgc tgatctgcta
    16981 tcgggccagg gcgcggatcg ccgggccgga ggggacgcgc gaggtcgaga tcgaggacgt
    17041 gtttgcgggt cccgggcgca ccaccctgaa gccgggagag ctcctggcgg ccatcgttct
    17101 tccgccgccg cagccgcgtt cggcagcgca ctatcatcgc ttcacccccc ggcgcgaaat
    17161 ggacatcgcc gtggcgggtg ccgggacctg gatcagcctg gatgagcaag gccgcatttc
    17221 cgacgcgcgc atcgcccttg cctcggtggc gccaacgccg ctgcgggcgc aagcggccaa
    17281 aaagcggctc atcggcgagg ctccgagccg cgctctgctg gaggaggccg ggcggcttgc
    17341 ctccgctgat gtgcggccga tctcggatac ccgcggcagt gcggattacc gccgaaccct
    17401 cgtttctgtc cttaccgcgc gtgcccttgc cgtctgctgc cgatcactgg gcgtggaggt
    17461 cgatgtccca tgaagaagct cattcaatgc accgtcaacg gcgacgagca cgcggtgctc
    17521 gccgacccac gggacacgct gctcgagctg ctccgggacc ggctggagct gaccggtacg
    17581 aaggagggct gcagcaacgg aaattgcggc acgtgcaccg tgctggtcga tggcgagccg
    17641 gtcaatgcct gcctgatgct cgccgtcgag gcgccggggc atgacatcgt gacgatcgaa
    17701 ggcatatcca gaggggcgga gctcaacccc attcagcagg cgctggtcga tcatggcggc
    17761 acccagtgcg gcttctgcac accgggaatc gtcatgtcgg ccaaggcgct gctggatgcc
    17821 aaaccgcgcc cgaccgaagc ggagatccgt catgccatcg ccggcaatct gtgccgctgc
    17881 acgggatacg acaaaattgt cagagcgatc gccgcagccg cggcggtagt ggcaggggag
    17941 tgagcctatg accacatcga gccctgtcga ggataagcga gagctgcggg tcgtcggcag
    18001 gcgcctgccg cgtgtcgatg cgcgagagcg ggtcaccggc aaggccgtct atcccgctga
    18061 cctgcatcta cgggacctcg cgcacgccaa gctgctccgc agcccgtacg cgcacgcccg
    18121 catccgtggg atcgacacca gcaaggccga ggcgctgaaa ggcgtcctcg cagtcgctac
    18181 cgccgccgat tttgtcgacc taccctttgg cgcatccgtc accatgggcg aaggcagctt
    18241 cgacgtctgg atcgtcaacc aggccaacat ggcgcgcgag aaggtgttct gggtcggcca
    18301 gcctgtcgcc gccgtggccg ccgtcgacgt gcatacggct gaagaggccc tgaagctgat
    18361 cgaggtcgac tacgagccgc tgccgccggt ggtcgatctc gccgaggcga tgaagcccga
    18421 cgcgcaggtg ctgcattcgc atgtcgtcac gaagggcgtc gagcctgcac ccaggtcgcc
    18481 gagcaacgtc tgctcgctca cgaccatctc acgcggcgat accgaagccg cattcgccga
    18541 ggcagccgcg actggtgaaa ttcaagtgaa agtcgacacg gcccaccagg gttatctcga
    18601 gcctcctgtc gtggtggcgc aggtcgatcc gaacgggttt gccaccgtgt gggcctcgac
    18661 ccaggggcag ttcaccgccg agctgatgct cgcgcgcatc ctcggccttc cgcaatcgaa
    18721 gatccgtgtg gtgccgctgg aggtgggcgg ggcgttcggc ggcaagatcg cgcttcacgg
    18781 cgaggctgtc gccgtcaagc tttcggaaaa gtgcggccgt ccggtgcggc tcatgctttc
    18841 gcgcgaggag gtgctgcaag gcggcaccgg cccggctgcg ggagcgatga tcgacattcg
    18901 cgtcgcggcg gacaaggacg gacggctgac cgcgatcaaa ggcacttatc gggttgatgc
    18961 aggcggattg cccggcatga gcccctcgct gctcatgcag gcctcggctg cgctctatca
    19021 gtgccccaac ctcaggctcg aaggctacga cgtcgtcacg aacaagccgc ggacggaggc
    19081 ttatcgcggg cccggcggga tccaggcggc gttcgccatg gagcaggcga tggacatcct
    19141 ctgccagcgc ctggacatgg acccgctgga gttccgcaag cgcaacgcct ccgtgaccgg
    19201 cagcctgatg ccgatcggga cgccgttccc gccgatcggc ctcacgacca tcctggagga
    19261 agtgggccgg cacccatgct ggacggagcc gctgcaaccc ggcaagcatc cccgcgggcg
    19321 cggtctggcg ctcggctatt ggcggggcac gtccatgacg tcagccgcgc acgtcacgat
    19381 cgcgggcgat ggccgcccga tggtgacgat ggggccggtc gacatctccg ggacgcgcac
    19441 cgcgatggct caggtcgttg ccgaggagtt cgggctctcc atcgatgacg tccacgtgag
    19501 caccggtgat tcgaagtcgg tgggctatag cgacgtcgct gcggggagcc gtgtcggccg
    19561 aaccacgact gcggcgctgg tcgaagcctg tgcggacgcg ctgcggcagc tgcggtcccg
    19621 agccgccgag aagctgcaga gccgacctga agacgtcgcc tatgccaacg gggttttccg
    19681 tgcgcccggc ggggcggcca tctcgttgtc agagctgatg gcagcgacat tgggggaagg
    19741 cgccatcgtc ggccgcggcg tctcgacgag gctgccgctg ggtgtcgaaa tcggcgcaca
    19801 cgtgtgcgac gtggaggtgg acccgggcac cggccaggtg acggttctcc gatacaccgc
    19861 cttccaggac gtgggccgcg ccctcaatcc tccggcggtc gaggggcaga tgcagggcag
    19921 cgtcgtgcag ggtctcggct gggcgctgac ggaaggattc gattacggcc ctgacggtcg
    19981 gctgaggaac gccagcctgc tcgactatcg cattccgacg gcgctggatg tcccgccgat
    20041 cgactgcgtc atcatcgaga cgccagtgcc gaacgtgcca tacggcgtgc gcggcgtcgg
    20101 cgaggtgccg atcgtgccgg ttgcgggggc cgtcgccaac gctgtcgccc gcgctatcgg
    20161 ggttcgtgtc ctgcagatgc cgatgacgcc agagcgagtt ctcaacgcaa tgcatgcagg
    20221 gatgccgagt tgatcggcat ccccaacccg cctcagttcg gctgtgagac gatgcggatg
    20281 taaggctttg gcgccttcca tccctgtgga tactttttgc gggcttcgtc gtccgaaacc
    20341 gagccggaga tgatcacgtc gtcgcccggc ttccagttgg ccggcgtcga gacgttgtgc
    20401 ttggcggtga gctgaagcga gtcgatcgcg cgcagcacct cgtcccagtt ccggccggtg
    20461 ctcatgggat aggtgaggat cagcttgatc ttcttgtccg ggccgatgac gaacacgttc
    20521 cgcaccgtcg cattgtcgtt gggcgtccgc ccttcggagg tgttccccgc atcggccgga
    20581 agcatgctgt agagcttgga caccttcagt tcagggtcac cgatcattgg ataattcggc
    20641 gcgacgccct gcgtctcctc gatgtccttc gaccagagct tgtggttttc cacagggtcg
    20701 acgctcagtc caacgagctt cacgccgcgc cgatcgaact ccggcttgat ctttgccagg
    20761 ctaccgagct cggtcgtgca gacaggcgtg aaatccttgg gatgcgagaa gaaaaggcac
    20821 caggaatcgc ccaaccattc atgaaaacgg atccggcctt cggtcgtatc ggcctcgaaa
    20881 tccggagcaa tactattgat ttgcagagcc attcaaatct ccttgttacc gcgagactgc
    20941 agttgcgcca ggtattgccg gcgcgtcgga tgatctgtgg cgaggtgccg tccgcgtgga
    21001 aattgcgccg gcgccagcca caatctgcaa tctcctgtat agcaccagcg caacacccgt
    21061 ttgcaaggaa ttaaccggcc tgcaatcaat ggctggcagg gagcacaacc agaggttatg
    21121 tctaacccga agcggtgata tcgaatgatg cggtgccgca atgagccttg ggggagtcgg
    21181 aatgggtacc gttgtcataa aatgtgacgc tacggggttg gatttttccg tgggaattga
    21241 aactgacgaa atcagtttcg gtgcccttcc ggatatccgt ctcaagaccc gttgcccaca
    21301 ttgcggcagc gagcacaact ggtccacgag acaggcgcgg ctgcggacgg attttccatt
    21361 cggggcggcg cgaagcgcgc catcgcaccc ggtcctcgtg acctcggcgg cgaagcgctg
    21421 aggcattcag atccgttatc gccaattgtg attcgcttgg cttggttccg aaaaacacgg
    21481 cttgggccgc tcctccggac attctagagt tcgcgccagc cacaggaaca acagcgaggc
    21541 tgagtgcgat gaaactgaaa ctgcaccatg tcaatctgac gacgagcaac gttcccgcca
    21601 tgactgagtt ctaccgtgac gtcctccagc tcgaggagga tccgcaactc gacgccaccc
    21661 gcgtgacctc tcaggggtat gcgggaaagg tcggcttcgt gacggacggg aacatgcaac
    21721 tgcacttggc cgaaagagac ttgaactgcg cctttcggac cggacacgcc atcaaccctt
    21781 tggagcgagg ccatatcgct tttcgaacgg acgacatcga ggccttcaag aagcggctca
    21841 aggaaaaggg catcccgttt tcggattatg gcgcttgggc gatgaacggc tgggagcaga
    21901 tcttctttca cgatccggat ggcaacatca tcgaggttca tcaggtaaag acctgattgc
    21961 atctaaccga tcctggtcac tggcgagttg atgtgtcggc aaccgctgtc atcatggcag
    22021 gcacagcgcg ccgaatagtg ttgacggaaa ccagcacaaa caaggagaga tgggcgcctc
    22081 gtcgggggga gaccgagagg cgccctcatt gccaccgatc gagactaggg ggcatgacca
    22141 gtgggcaatc gtgaaacgcg gagtgcccgg aatggttcca catcggacga tcttacttaa
    22201 ctataactcg cagctgtggc ttgcttgcca ctaggcaggc gccggtctgc ctgccgcaaa
    22261 ccgcggcatc gagtcaattg catttcagaa cgattcaatt tcgtcggcgt ttccttcatt
    22321 gcatctgcta caataaggga gccgcctggc ggatcaagtg gacaagaagc cggtgcttcc
    22381 gcgcgatata gtcgatcaca ttgcggcgtt cgtcggagtt cttgacctgg acggaacggt
    22441 tgcccaggtc aacgagacga cactgaaaat ttccggttca caacgccatg agctgatcgg
    22501 gacgaagctg tgggatctct catggttcac cagcgatccc gagagcgccg ctcgtgtcaa
    22561 ggacgccgtg acccgcgccg cgaagggcga gacggtacgg ttcgacacga cggtcacgac
    22621 ccacgggggc gagcgtctgg caatcgacct catgatccac ccggttctcg acgagaagac
    22681 gggcaaggtc caatacctcg ttccctcggg gatcaatatt acacgccgaa aagcgatcga
    22741 ggccgagctg aaacgcagcc gtgatctcct cgagcgggcg cagcgggtcg gccatctcgg
    22801 acattggagc ttcgacctgg ctacaaagaa gctggagtgg tcggccgaag cacggcgcat
    22861 cttctttggt gaggaggagt cccgcgaggt ttcggcggaa acgatcgaga aactgatcca
    22921 tccagatgat ctggagcggg tacgcgcggg gatgatggct gcgatccaag caggcgagag
    22981 cttcgcaacg tcatatcgca tccgccgcgg cgacggtgag gagcgcatca tagagctcca
    23041 ggcggatccg gattacgcac ctgacggcga gctactccgc gtgttcggca tcgtccgcga
    23101 cgtgactcag gctcatttgc gcgaacagca gctgatcgag cagcaacagc tcatcgatct
    23161 ctcgctggag cccatcctgt gctggagcct tgacgatgga ttgctccatt ggaacccggg
    23221 ctgccagcat ctctacggct actctcgcga ggaggccctt ggacaccgaa tcgatgagct
    23281 gctgcattcg cagttccccg tccctatcgc caaaatcaga aagcgcttgg aggctggtga
    23341 gtcctggacg ggcgaggtga tccagacagc aaaagatggt cgcaaaatca tcgtcgaggc
    23401 gcggatcgat cccgtcgttt ccggcgagcg caggatcgtc atggaagcgc atcgcgatat
    23461 cacggcccgc cgccaggcag agcaggcact caagttgagc gaggagcgcc ttgcgatcgc
    23521 caccaagttg gcgggcgtgg gatttttcga ccacgatcaa atcgccgatg tcatttgttt
    23581 ctcgcccgag ccatgggcgg gaatgcttcc tacggaggcc acccttgatg atgtgttcgc
    23641 gatcgttcac ccggacgatc tcgcgccatt tcgcgaagcg atcgccaaag cacatgatcc
    23701 ccgcggcaca gggcagctcg agcacgaata ccgtatgatc cgcaaaaccg gcgaaattcg
    23761 ctggatcagc gtgaagtcgc gtacattttt cgagggagag gggaaagagc gacgcccggt
    23821 ccgaactgtg ggtgcagtcg ttgatgtcac cgagcagcgg atgtgggcag agcagcaacg
    23881 cctcctgatg ggcgagctca accaccgcgt ccgcaacacg ctctccgtcg tgcaggcgat
    23941 cgcgacccag accttgcgca ccgccaagga cccgcaagcc ttcgtgcaag atttcaaagg
    24001 ccgccttcag gccatcgcca gtgcgcacaa gctcctgaac gagacgacct ggcaaggagc
    24061 aaatctcacc ggtctcgtcc gagagcagct cgcttcgagc tgcggctccg gtcaagttta
    24121 caccgatggg cctgaagtct ggctgcctcc gcaggtcgcc ctgaatttag ggctcgtgct
    24181 ttacgagctt gccaccaacg cccgcaagca cggtgcgttg tcggtggcga acggacgcgt
    24241 gcaactctcc tggcaagtgg tgcgcgagga cgacaagcgt gtgctgcgcc tgacctggca
    24301 ggaatgtgga gggccggcgg ttcggccgcc aatctcgcgt gggttcggca tggggctgat
    24361 cgagcgcacg atgggcagcg ctgcggacgg cgcggcgagg atcgcgttcg agccgggagg
    24421 cctgcgctgt gtcattgaca tagcgctgca gtctgcggag cgatttgcca tctcagctcc
    24481 ctgagccgtt gcccggccgt tcgatttcag ggctcggccg ccccaatttt gcagacggat
    24541 tgagccgctt ggatcaagca ggccccgcga cggccttgct ctccacgagc gcaatgagcc
    24601 ccttgagcat gtccgtcggg ctcgggggac atccgcggat atgcagatcg accggtatga
    24661 cgtcggagac gccgccggcg caggcatagc tgcccgcgaa gagcccaccg ttcaatgcgc
    24721 aatcgccgac ggcgacgacc catttgggat cgggggtcgc gttataggtc cgctccagcg
    24781 cctcgcgcat attcttcgtg accggtcccg tcaccagcaa tacgtcggca tggcgcggag
    24841 aagcgacgaa gcggagtccg aagcgctcga ggtcgtagta gatattgttg agcgcgtgta
    24901 tctcgagctc gcacccgttg caggagccgg cgtccacctg ccgaatgg
//
LOCUS       DASVDU010000061         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1273224, whole genome shotgun sequence.
ACCESSION   DASVDU010000061 DASVDU010000000
VERSION     DASVDU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273224"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..596
                     /locus_tag="VF226_04160"
     CDS             255..596
                     /locus_tag="VF226_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073212.1"
                     /translation="MGLFARLKSAQAYWKQHLHFLHGSEDLELLVEIGFHQERGQPLS
                     MKQLYLANGASVATVQRRLQRLRAAGAIDCGRTERDARLAELRVNPRVVKLLRRFGDL
                     HAPEAGATKES"
     gene            871..2307
                     /locus_tag="VF226_04165"
     CDS             871..2307
                     /locus_tag="VF226_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX7073213.1"
                     /translation="MRAGFLREVIRGWYIPARPDELQGDTTAWYASMREFVAGYAQSR
                     FGDEWHVNPEQSLLLRSGDRTVPRQLQIWAHKGTNQTVQLLHGCSLFIYQAPKLLPSS
                     PVADCGRLRLVEMPFALVHASPTVFAQHPIAGQIALASLPDASDVLRIVLEGPHPSVA
                     GRLAGALRAIHRSALADEIVGAMKSAGYAVNEVNPFERPLPAILPGGRPESPYVQRLR
                     LTWAQMRETVIKAFPQRPPPPGDVEGLLKDVEARYVTDAYHSLSIEGYRVTATLIDKV
                     RDGNWNPDGDEKDRGTRDAMAARGYFETHKLVTEDLVRAIEGENSGTVFRNALPRWYQ
                     ALFSPSVQAGILKPADLAGYRNDQVFIRGALHVPLSKEAVRDCMPVLFELLEAEREPA
                     VRAVLGHFLFVYIHPYMDGNGRLGRYLMNLMLVSAGYTWTVIPVQQRDEYMKALEQAS
                     SFGNIEPLARFIAGLARAQAKAPPPAPE"
     gene            2304..>2504
                     /locus_tag="VF226_04170"
     CDS             2304..>2504
                     /locus_tag="VF226_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073214.1"
                     /translation="MNGNIRREKALNEKSLEHTPMMQQYLGIKAEHPDILVFYRMGDF
                     YELFYDDAEKAARLLDITLTQRG"
BASE COUNT          482 a          810 c          764 g          448 t
ORIGIN      
        1 accgcccaga cgaacgcggc gatcagcgcg caaagcaccg cgacaccggc gcggccgggc
       61 tgaccgtaca ccgtcggctt tcccatcccg ccggctcccc cccgggagcc ttttcttgtt
      121 gtgtgaggca gccatcctaa tccgcctcaa aaatgagttg caatatcagt tgatatggat
      181 caagcctgcg cttatccctt ggccttaaat acctgtcggg attttcccga cagcaaggca
      241 aggacaaggg tgggatggga ttgttcgcaa ggctgaagtc ggcgcaggcc tattggaagc
      301 agcatttgca ctttctgcac ggctcggagg atctggagct cctcgtcgaa atcggtttcc
      361 accaggagcg gggccagccg ctctccatga agcagctcta cctcgcgaac ggcgcctccg
      421 tcgccaccgt gcagcggcgc ttgcagcggc tgcgcgcggc aggcgcgatc gactgcgggc
      481 gcaccgagcg cgacgcgcgc ctggcggagc tccgcgtcaa tccgcgcgtc gtcaagctcc
      541 tgcgccgatt cggcgacctg catgcgcccg aggcgggagc gacgaaagag agttgaagag
      601 cctcgcccag gcgggcagtc agcggaccga ggtcaggcgt caaggcaagt ctctatttat
      661 aaataggatt tacaatatac tcttgtatcg aaaatcaata gcctcttgtg ctgaaaagca
      721 ctagactctt gtgtccactt gcaactggct attgtgtctg cgcaaagaga acgtctggca
      781 gccggcttgg cggccctgaa gagccttcag gaccgagggc tacacgccgt ccctggtccg
      841 gagctctccc gcaaagaccg ggaggccctc ttgcgggctg ggttcctcag ggaggtcatt
      901 cgcggctggt acattcctgc ccggcccgac gaacttcaag gcgataccac cgcgtggtac
      961 gccagcatgc gggagttcgt cgccggctat gcgcaaagcc gcttcggcga cgaatggcac
     1021 gtaaacccgg agcagtctct gctactacgc tccggggatc gcaccgtccc caggcaactt
     1081 cagatctggg cgcacaaggg gaccaaccag accgtgcaac tgctgcatgg ctgttcgctc
     1141 ttcatctatc aagcgccgaa actgctcccc tcatcgcccg tcgccgactg cggccgtctt
     1201 cgcctcgtgg aaatgccttt cgcgctcgtc cacgcgagtc cgactgtctt tgcccaacat
     1261 ccgatcgccg ggcagatcgc cctcgcctcc ttgcccgacg cctcggatgt gcttcgaatc
     1321 gttctcgagg gtccgcatcc ttccgtcgcc gggcgccttg cgggcgccct gagggcgatc
     1381 caccgatcag ccctggcgga cgaaatcgtt ggtgcaatga aaagcgccgg ctacgctgtc
     1441 aacgaggtga acccgttcga gcggccattg cccgccattc tgcccggcgg ccggccagag
     1501 tcgccttatg tgcagcgctt gcggctcacg tgggcgcaga tgcgtgagac ggtaatcaaa
     1561 gcgtttccgc aacgtccgcc accaccagga gatgtcgagg gcttgttgaa ggacgtagaa
     1621 gcccgctacg tcacggatgc ctaccactcg ctgtctatcg agggctaccg cgtcaccgca
     1681 acgctgatcg acaaggttcg cgacggcaac tggaaccccg atggcgacga gaaagaccgc
     1741 gggacccgcg atgccatggc ggcccgaggg tatttcgaga cgcataagct cgtaacggaa
     1801 gacctcgtcc gcgcgatcga gggagagaac tccggaacgg tcttccggaa cgctctgccg
     1861 cgctggtacc aggcactgtt cagcccgagc gtccaggcgg gcattctcaa gccggccgac
     1921 ctggccggtt atcgaaacga tcaggtgttc atacgcggcg cgctccacgt gccgctctcg
     1981 aaggaggcgg tgcgcgattg catgccagtc ctcttcgagc tgctcgaggc ggaacgagag
     2041 ccggcggtgc gggcggtcct cgggcacttc ctcttcgtct atatccatcc gtacatggat
     2101 ggaaacggca ggcttggccg atacctgatg aacctcatgc tcgtgtcggc gggatacaca
     2161 tggaccgtga tcccggtcca gcagcgcgac gagtacatga aggcgctcga gcaggcgagc
     2221 tcgttcggca acatcgagcc tctcgcgcgc ttcatcgccg gcctggctcg cgcacaggcc
     2281 aaggcaccgc cgccggcccc agaatgaacg gaaacatccg ccgggaaaaa gcgttgaacg
     2341 agaaaagcct ggagcacacg ccgatgatgc agcagtacct gggcatcaag gccgagcatc
     2401 ccgacatcct ggtgttctac cggatggggg atttctacga gctcttctac gacgacgccg
     2461 agaaggcggc gcggctgctc gacatcacgc tcacccagcg cggc
//
LOCUS       DASVDU010000062        14128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_304575, whole genome shotgun sequence.
ACCESSION   DASVDU010000062 DASVDU010000000
VERSION     DASVDU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14128
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304575"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..177
                     /locus_tag="VF226_04175"
     tRNA            103..177
                     /locus_tag="VF226_04175"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136..138,aa:Glu,seq:ttc)
     gene            complement(452..2071)
                     /locus_tag="VF226_04180"
     CDS             complement(452..2071)
                     /locus_tag="VF226_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073215.1"
                     /translation="MTNKTGWMSRRDLAKLLGVGGAAIVAGLPERVFAASDKTTLVIG
                     IDISDTITLDPARQAQYTPPMSLRAAYDMLVTMSPGDYVNLKPALATKWERTPDGKGW
                     RFTLREGVKFASGNPMTAEDVKWSIDRVLYIGDQPSQYISHLDRTEIVDAKTVDLILK
                     DPSQPILNILAAPGFPVYDRKLVEQHGGDASKDAKTKDKATTWLNSNSAGTGAYKLVR
                     WERNSQIQFVRNDHYWGGKPPFQRVVIRHIADSAAQLLAIRRDDIDIAFNLIPEQIAT
                     LKEEPNVRLEGLTSLDFVYMALTQEPEFNKALAIKEARQAIGYAIDYDGIIHGLLGGA
                     AVRPASFLPIGVSGSTEEIAKEISFRHDPDKARQLLQKAGLADGFEFELAYGNAAIAG
                     LNYQTLGQKIQSDLAKVNIRVKLAPMDQVNLRTTYTGGKAQGGVLTFWNPPAVENLLW
                     AAATVERVAKRVHWTPPEDLVKLVHQAAAEEDQKKQADLWVEYQKAMVDQANLIVLFQ
                     PIYQIAVRDTLGKLPLTAAGWQLDMEAVKPA"
     gene            complement(2219..2380)
                     /locus_tag="VF226_04185"
     CDS             complement(2219..2380)
                     /locus_tag="VF226_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073216.1"
                     /translation="MDFTTSENGPVIGAGGDDVGHAIAMALTRDGFAAERYVKLFVML
                     TRIFALGEL"
     gene            complement(2432..3613)
                     /locus_tag="VF226_04190"
     CDS             complement(2432..3613)
                     /locus_tag="VF226_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7073217.1"
                     /translation="MNDVHSGSIELRLPSTDGSLSTYRLTGEPLPIAKPARAFNRVAF
                     AAAHVVADPFTAVEPSDKPVLDWPRTIAYRRYLIGLGLGVAEAMDTAQRGMGLDWHGA
                     LELIGRTLAETRDLPDALIFSGCGTEQLDPADARTIDDVIAAYLTQMHAIQKLGGRII
                     LMASRALARVARSPEDYRKVYARVLSEADQPVILHWLGDMFDPALAGYWGGDRFEETV
                     ETALGVIAENAAKVDGIKISLLDDAKEIAMRRRLPAGVKMYSGDDFNYPELIAGDAHG
                     YSHALLGIFDAIAPAASSALAALAAGDRARYDALLAPTVPLSRLIFRAPTRFYKTGVV
                     FLSYLNGHQDHPIMVGGQQGMRPLPYFCEIFKLADQAGLLADPADATARMRRFLAFYG
                     V"
     gene            complement(3610..4755)
                     /locus_tag="VF226_04195"
     CDS             complement(3610..4755)
                     /locus_tag="VF226_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7073218.1"
                     /translation="MPLSETHEEIRSLARRFADEVIRPQAEALDRDEAFPADIYRQMG
                     ELGLFGITVPAELGGAGLDTLAYALVMEELSRGYASIADQCGLYELIGTLLSVHGTPA
                     QQQKWLGPLLSARVKPAYCITESGAGTDVSGIQTTAERTATGWRLSGSKLWIHNAPVA
                     DVGFVLARTDKDAGKRGMSIFIVPFDLPGVSRGPKEHKMGQRASQVGALHFDQVEVPA
                     DALLGAEGRGFHIMMSVLDKGRIGIAALAVGIAQAGLEAAVAYAKERKQFGQPIADFQ
                     GLQWMLADMAKDITAARLLVRNAAEALDSGRPATIYCSMAKCFASDMAVAQTANAVQV
                     FGGSGYIRGFEVERLYRDAKITQIYEGTNQIQRSIIARELLSKGVPA"
     gene            complement(4773..5213)
                     /locus_tag="VF226_04200"
     CDS             complement(4773..5213)
                     /locus_tag="VF226_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7073219.1"
                     /translation="MSDIPVKIGEEAQFSKTIGEADLLLFSGLSGDFDPIHVNEEYAR
                     KSQFGRRIAHGALVMSLLSTTASMISRRAVERGSKGVSVSLGYDKIRFLRPVFIGDTL
                     TATYRVEELDEAAGRHRARVEVANQNGELCLAGTHVMRWLPPSA"
     gene            complement(5210..6028)
                     /locus_tag="VF226_04205"
     CDS             complement(5210..6028)
                     /locus_tag="VF226_04205"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073220.1"
                     /translation="MSAPLQALIGGSTRLFAIIGHPIAQVRSPQTFNPRLAAAGHDAV
                     LVPMHVEPDHFDETVRGLKRLANLDGLIITVPYKSRILPYVDRVLPMAEAVGAVNAMR
                     REPDGGWSGDIFDGVGLMHGMREQGEDPRGRRVMLLGAGGAGSAVAVALAQAGAAAVT
                     IHDVDAGRAEALAARVKRLYPSCNTRAGAPDLNTHDTLINATPIGMAEDDPLPIPIGQ
                     LRPEMLVVDIIMKPEITRLAAAARERGCKTLPGKTMLEGQATELMRFFGVEEGT"
     gene            complement(6031..6264)
                     /locus_tag="VF226_04210"
     CDS             complement(6031..6264)
                     /locus_tag="VF226_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073221.1"
                     /translation="MQRVTAEEFIELIRSGDTSLIGGSGNGHAVPELPTFDTIGARLF
                     PPEPIALAAHLAGCTPRACSTPLAAAALSIAGE"
     gene            complement(6280..8364)
                     /locus_tag="VF226_04215"
     CDS             complement(6280..8364)
                     /locus_tag="VF226_04215"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073222.1"
                     /translation="MTKTLKAELGRVATGADAPALEVKNLHTQFFTDDGVVSAVDGVS
                     FTVKQGEALGIVGESGSGKSVTALSLMRLLDEPARITEGEVRFQGRDVLAMSPEELRS
                     VRGDRMAMVFQDPMTSLNPVLKIAKQLIETMVVHGRFTEAEASKRAVDLLGRMGVTAP
                     ERAVESYPHQFSGGMRQRVMLALGMSNEPALLIADEPTTALDVTIQAQILDLIRELNR
                     DYGTSVVLISHDLGVIARVCHRTIVMYSGEVVEEGLTEDLLADPRHPYTWALINAVPR
                     IDEARPLDRRLTTIEGQPPDPLNLPQGCRFAPRCPFRVEKCDVHPDLAEVAPGRKARC
                     WVTQAGTTLPKKTLRATAASAEATPPRVIAGRDEAPLLKLRDVVKHFPLPKTGYFQPR
                     RVVHAVDNVDLDVFRGETVGLVGESGCGKSTIARLVMRIHQPDSGEIIFDGHDIAKST
                     QKQIRPLRRRMQMVFQDPYGSLNPRMTVGDIIAEPLLFHGITKGKAETQERVDELLNI
                     VGLNPRVGRRYSYEFSGGQRQRVSIARALAVRPDFIVADEPISALDVNIQAQIINLML
                     DLQERFGLTYLFIAHDLAVVRHISDRVVVLYLGKVMEIAPADRLFDRPLHPYTRYLLS
                     AAPIPDAAVERERGYLKLEGEPPSSISPPSGCRFRTRCPLAKPICASEPPPLVEHEPG
                     HLAACHFAGQLK"
     gene            complement(8372..9250)
                     /locus_tag="VF226_04220"
     CDS             complement(8372..9250)
                     /locus_tag="VF226_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073223.1"
                     /translation="MSVAGEAAAARRIAPVPTKAQRWRRRYGLPAVGMFIILVWIVVA
                     ITAPLLSPYDPNLVDVSNRLKPPSGEHWLGTDALGRDVFTRLLYGSRISLTTGFVVVL
                     VGAFFGTLLGGIAAFARGKVEEIIMRLTDLVLCFPPIILALAIAAALGIGTVNTIIAM
                     LAVWWPKFARLAHSMVLTQRSQEYVEAAVVAGFGPGRILFRHIIPNSIGPLIVLVTLD
                     IGNAVITFAGLSFLGLGVVPPTAEWGSMVAEGRELVAQWWVATFPGFCILSIVLGFNF
                     LGDGVRDWLDPKARKR"
     gene            complement(9247..10257)
                     /locus_tag="VF226_04225"
     CDS             complement(9247..10257)
                     /locus_tag="VF226_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073224.1"
                     /translation="MTRYIVSRLGITVVMAVLATLVIFLIANTVPGDPVLAQLGDVAA
                     SDEKFVAEWRTKFGLDLPLWQRYLIFLSGLVHGDLGISIASQRPVLDDIIQFAPATIE
                     LATIGFVLALLIGIPLGIIAAVWRDTWVDHIARVVSLIGVSSPTFWLAFIMLAIFYGG
                     LEIAPGPGRLDPIALPPERVTGLLLIDTAIAGDWETFRDAAAHLVLPSIVQAAATLGL
                     ITRTTRASMLESIQQDYVRVARAKGLKKSTIILGHVFPNAVIPVVTLGGLAYANLLSG
                     AVMTETIFSWPGLGLYTFRSAAALDFPAIMGITLVVALVYLIINLVIDISYAFLDPRV
                     TR"
     gene            complement(10485..11528)
                     /locus_tag="VF226_04230"
     CDS             complement(10485..11528)
                     /locus_tag="VF226_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7073225.1"
                     /translation="MDDRPKPVRPRERLTLQDLAAHAGVSRATVSLVLANSPLVAEKT
                     RARVLESVRVLGYVYNRGAANLRTRRTHTIGIAINEITNPYFTELTAVLQGAFRDLGR
                     TVFVSNSDEDPARQDQFIATMREYNADGLVICPAEGTEAESLRRLKEQGIACVLLSRD
                     VPGSGLDYAGHAHRQGTSMVTEHLIGLGHRRIAMIGGADRNWTGRERRLGYRDALAAN
                     GLPLAPELVLAGTPSRSFGAEAIKRLMRLDDPPTAAVCFNDVIAFGVMLGLRQIGREP
                     GRDFAVFGYDDLAEAELWTPALSTVRIDTHAIGTAAAKLLLERIENPDAPPKRVISQP
                     SLIIRESSCPRPR"
     gene            11576..12343
                     /locus_tag="VF226_04235"
     CDS             11576..12343
                     /locus_tag="VF226_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7073226.1"
                     /translation="MSDEIRTVKGDFIAEIILDRPSKMNAITPAMAAAIEHHCREFDR
                     DDSVRVVLLRGAGDRAFCAGSDLNALAEYPTPWKFRNRVEYATAVRNMRKPVVAALHG
                     WVLGGGAEMALSADIRVMGRSARLGFPEVKRGWVGGGGASQMLPRLIGYGQAMRLLLL
                     GEPLDAEEAMRLGIVEFLVDDAEVEAKARDICRTIASYSPVAVESVKAAVRMALQSDL
                     ASGIRYENEMNTLCFSAGDHMEGIRAFQEKREAVFKA"
     gene            complement(12485..13603)
                     /locus_tag="VF226_04240"
     CDS             complement(12485..13603)
                     /locus_tag="VF226_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7073227.1"
                     /translation="MTRRVASPIELSADERDVLERRIRRRKIARAEAQRAEIILRAAD
                     GENNCQIADAVGVTRQTVRTWRERFAKDRLDGLGDEPRCGAPRKIGDDRIEAIVVKTL
                     EEKPANATHWSTRGMAKASGVSTSTVHRIWRAFSLQPHRSETFKLSADPQFVEKVRDI
                     VGLYLAPPEKALVICVDEKSQIQALDRTQPLLPLRPGQIERRTHDYERHGTTTLFAGL
                     IASVTAKADVKAGTVIGKCMPRHRAREFRTFLDEIERNVPDRFDIHVVMDNASSHKTK
                     LIRDWFAKRPRWHMHFTPTSSSWINQVERFFALLAEKQIKRGAHRSVAALIKDIEAFI
                     ERQNADPRPLRWTKSADDILASIERFCRRTLQVHVPPG"
     gene            complement(13915..>14128)
                     /locus_tag="VF226_04245"
     CDS             complement(13915..>14128)
                     /locus_tag="VF226_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter permease"
                     /protein_id="HEX7073228.1"
                     /translation="LEENKFPLAPMVLGIVLGKLVEQHLMQSLISTQGSIVGLFERPM
                     AGILGAITLAVWLFPFVRWVWNRARG"
BASE COUNT         2614 a         4502 c         4504 g         2508 t
ORIGIN      
        1 tcgggcagtt tccgcgcggc cggcacaccg ggaggtgcgg cgcggaaacg ccacctgctc
       61 ccttgccagc cccctgaaac ccccctatac cctgcgccca gcgctccctt cgtctatcgg
      121 ttaggacgcc agattttcaa tctggaaaga cgggttcgac tcccgtaggg agcgccagtg
      181 tccgaatagc ccactattct gtcccccgat ttttggcctg tccggggaca taggcctctc
      241 ttcgctacgc tacagcgtca cgctgtcagg gatgcggccc cacctcgcag gaacaattcg
      301 agccgcggtg ttcgggatcg atagttgccc aggaagcagg gcgcccatcg cgacgacacc
      361 tcaccacgag tggctcatgg tgaggcgtcg cagatgcctt ctttccgcgc aactgcctgg
      421 gtgcgagtac gcggcccctc ctctggccag atcaggccgg cttcaccgct tccatgtcga
      481 gctgccagcc ggcggcggtt agcggcagct taccaagcgt gtcgcgcaca gcgatctgat
      541 agatcggctg gaacaggacg atcaggttgg cttggtccac catcgccttc tggtactcga
      601 cccagagatc ggcctgcttc ttttgatcct cttcggccgc ggcctgatgg acgagcttca
      661 ccaagtcctc tggcggcgtc caatgcacgc gcttggcaac gcgctctacg gtcgccgccg
      721 cccagagcag gttctcgacc gcaggtgggt tccagaaggt cagtacgccg ccttgcgctt
      781 ttccccccgt atatgtggtg cgcaggttga cctgatccat cggcgcaagc ttcactctga
      841 tattgacctt ggcgaggtcg ctctggatct tctggccgag ggtttgatag ttcaggccgg
      901 caatggcagc gttgccatag gcgagttcaa actcgaagcc atcagcgagc ccagctttct
      961 gtagcagctg cctggctttg tccgggtcgt ggcgaaagct gatctccttg gcgatctcct
     1021 cggtcgagcc agaaacgccg atcggcagga agcttgctgg gcgaaccgcc gcaccgccga
     1081 gcaggccatg gatgattccg tcatagtcga tggcgtagcc gatcgcctga cgagcctcct
     1141 tgatggcaag cgccttgttg aactccggct cctgcgtcag cgccatgtag acgaagtcga
     1201 ggctggtcag cccttcaagg cggacattcg gctcctcctt caacgtggcg atctgctccg
     1261 ggatcagatt gaatgcgatg tcgatgtcgt cgcggcggat ggcgagcaac tgcgcggcgc
     1321 tatcggcgat gtggcggatg acgacgcgct ggaatggcgg cttgcccccc cagtaatggt
     1381 cgtttcggac aaactggatc tgggagttgc gctcccagcg caccagcttg taggcgccgg
     1441 tccctgcgga attgctattc agccaggtgg tcgccttgtc cttggtcttg gcgtccttgc
     1501 tggcgtcgcc accatgctgc tcgaccagct tgcggtcgta gaccgggaaa ccgggtgcgg
     1561 cgagtatgtt caggatcggc tggctcgggt ccttgaggat cagatcgacg gttttggcat
     1621 cgacgatctc agtgcggtcc aggtgcgaga tatattggga aggctgatcg ccgatataga
     1681 gaacgcgatc gatcgaccac ttgacgtcct cagcggtcat cgggttaccg ctggcgaact
     1741 tgacaccctc gcgcagtgtg aagcgccaac ccttgccgtc cggcgtcctc tcccatttgg
     1801 tggcaagcgc cggcttcagg ttgacatagt cgcccggaga catcgtcacc agcatgtcgt
     1861 aggcggccct gagcgacatc ggcggcgtgt actgcgcctg acgggccgga tcgagcgtga
     1921 tggtatcgct gatgtcgatc ccgatcacca gggtggtctt gtcgctcgcg gcgaaaacgc
     1981 gttcgggcag acccgcaaca atcgccgcac cccccacacc cagcaacttc gccaagtccc
     2041 gccggctcat ccagcctgtc ttattcgtca tgcttctctc ccttcgattc tcagcacgtc
     2101 acttggtcga agtctcttct ttggatcgat ctaatgaacc aaaaaagcaa actccgcagc
     2161 tagatttacc ggatcctatt cgcacattta ggcattatca attgattaca tttcccaatt
     2221 acaattctcc aagcgcaaaa atccgcgtca gcattacgaa cagcttgaca tagcgttcgg
     2281 ccgcaaagcc atcgcgtgtc agcgccatcg cgattgcgtg gccaacatcg tcgcccccgg
     2341 cgccgattac cggcccattc tcgctcgtgg tgaaatccaa gccgcgctgg cttcaccctg
     2401 ggcgcagaat tatgcggtga gccttcatgg ctcagacgcc gtagaaggcg aggaagcgcc
     2461 gcatacgcgc cgtcgcatca gccggatcgg cgagcaggcc ggcctggtcg gccagcttga
     2521 aaatctcgca gaaatagggc agcggccgca tcccctgctg gccaccaacc atgatcggat
     2581 ggtcctgatg gccgttgaga tagctcaaga acaccacacc ggtcttgtag aaacgcgtgg
     2641 gcgcccggaa gatgaggcgc gacagcggca cggtcggtgc cagtagcgcg tcgtaacgcg
     2701 cgcgatcacc ggctgcgagc gcggcaagcg cgctcgaagc ggccggcgcg atggcgtcaa
     2761 aaatgccgag cagcgcgtgc gaatagccat gcgcgtcgcc ggcgatcagc tcgggataat
     2821 tgaaatcgtc gccgctgtac atttttacgc cggccggcag acggcggcgc atcgcaatct
     2881 ccttggcgtc gtcgagcagc gatatcttga tgccgtcgac ttttgccgcg ttttcggcaa
     2941 tcacgccgag ggccgtctcg acggtttcct cgaagcggtc cccaccccag tagccggcga
     3001 gcgctggatc gaacatgtcg ccgagccagt gcagtatgac cggctggtcc gcctccgata
     3061 agacacgggc atagaccttc cgatagtctt ccggactcct ggcgacccgg gccagcgcgc
     3121 ggctcgccat caagatgatg cggccgccga gcttctggat ggcatgcatc tgcgtgaggt
     3181 aggcggcgat gacgtcgtcg atcgtgcgcg catcggcagg atcgagttgc tcggtgccgc
     3241 accccgagaa gatcagggca tcgggcaggt cgcgcgtctc ggcgagcgtg cgcccgatca
     3301 actcgagcgc gccgtgccag tcgagcccca tgccgcgctg ggcagtgtcc atcgcttcgg
     3361 cgacgccgag cccaagcccg atcaggtacc ggcgataggc aatggtgcgc ggccagtcga
     3421 gcaccggctt atcgctcggc tcgaccgccg tgaacgggtc ggcaacgaca tgcgccgccg
     3481 caaatgcgac ccggttgaag gctcgcgccg gcttggcgat cggcagcggc tcgccggtca
     3541 ggcgataagt cgacagggag ccatcggtcg atggcaggcg tagctctatg gaccccgaat
     3601 gcacgtcgtt catgccggca cacccttgct caaaagctca cgcgcgatga tcgaacgctg
     3661 gatctgattg gtgccctcgt agatctgtgt gatcttggcg tcgcgataga ggcgctcgac
     3721 ctcgaagccc cgaatatagc cgctgccgcc gaacacctgg acggcattcg ccgtctgggc
     3781 caccgccatg tcgctggcga agcatttggc catcgagcag tagatcgtcg ccgggcgccc
     3841 cgaatcgagc gcttcggccg cattgcgcac caacaggcgt gcggcggtga tgtccttggc
     3901 catatcggcc agcatccact gcaggccctg gaaatcagca atgggttggc cgaactgctt
     3961 gcgctccttg gcgtaggcga cagccgcctc gaggccggcc tgggcgatgc cgacagcgag
     4021 cgcggcgatt ccgatgcgcc ccttgtcgag cacgctcatc atgatgtgga agcccctgcc
     4081 ctcggcgccg agtagcgcat cagcgggcac ctcgacctgg tcgaagtgca gcgcgccgac
     4141 ctggctcgcg cgctggccca tcttgtgttc cttcgggccg cgcgacacgc ccggcagatc
     4201 gaacggcacg atgaagatgc tcatgccgcg cttgccggcg tccttgtcgg tccgcgccag
     4261 cacgaatccg acatcggcga ccggggcgtt atggatccac agcttggatc ccgacagcct
     4321 ccagccggtg gcggtgcgct cggcggtggt ctgaataccg gagacgtcgg tgcccgcgcc
     4381 cgattcggtg atgcagtaag ctggcttcac cctggcgctg agcaggggcc cgagccattt
     4441 ctgctgctgc gccggcgtgc cgtgcaccga aagcagggtg ccgatcagct catacaggcc
     4501 gcactggtcg gcaattgaag catagccgcg cgacaattcc tccatcacca gcgcataggc
     4561 cagcgtatcc aatccggcgc caccgagctc ggccggcacg gtaattccga acagaccgag
     4621 ctcgcccatc tggcgataga tgtcggcggg gaaggcttcg tcccggtcga gcgcttcggc
     4681 ctgggggcgg atcacctcgt cagcaaatcg gcgagcgagg gagcggattt cctcgtgagt
     4741 ctctgagagc ggcatgatcg gagctgcttc tttcaggcag acggtggcag ccagcgcatg
     4801 acatgcgtgc cggcgaggca gagctcgcca ttctggttgg cgacttcgac cctggcgcgg
     4861 tggcgccccg cagcctcgtc gagttcctcg acgcgatagg tcgccgtcag cgtgtcacca
     4921 atgaataccg ggcgcaggaa ccggatcttg tcgtagccga gcgacactga aacgcccttc
     4981 gaaccgcgct cgacggcacg gcgcgagatc atcgaagctg tggtcgagag caggctcatc
     5041 accagcgcgc catgggcgat gcgccggccg aactggctct tgcgggcata ttcctcgttg
     5101 acatggatcg gatcgaaatc gcccgacaga cccgagaaca gcagcaaatc cgcttcgccg
     5161 atggtcttgg agaactgggc ctcctcgccg atcttaacgg gaatgtcact catgtgcctt
     5221 cctccacccc gaaaaacctc atcagctccg tagcctgccc ttcgagcatg gtcttgcccg
     5281 gcagggtttt gcagccgcgc tctctggcag cggcagccaa gcgcgtgatc tccggcttca
     5341 tgatgatgtc gaccaccagc atctcgggcc gcagctgccc gatcggaatg ggcagcgggt
     5401 cgtcctcggc catgccgatc ggcgtggcat tgatcagcgt gtcgtgcgtg ttgaggtccg
     5461 gcgcaccagc acgtgtgttg cagctcggat aaagtcgttt gactcgcgcg gcgagcgcct
     5521 cggccctgcc agcatcgaca tcatggatgg tgacggccgc tgcacccgcc tgcgccagag
     5581 ccaccgcgac ggcgctgccg gcgccgccgg ctccgagcag catgacgcgg cgaccgcggg
     5641 gatcctcgcc ttgctcccgc atgccgtgca tcaggcccac accgtcgaag atatcaccgc
     5701 tccagccgcc gtctggctca cgccgcatcg cattgacggc gccaacggcc tcggccatcg
     5761 gcagcacacg gtcgacgtac gggaggatgc gcgacttgta gggtactgtg atgatgagac
     5821 cgtcgaggtt ggcgagcctt ttcagaccgc gcaccgtctc atcgaaatga tccggctcga
     5881 catgcatcgg caccagcacc gcatcgtgcc cggcggcggc aaggcgcggg ttgaaggtct
     5941 gcggcgacct tacctgggca atcggatgac caatgatggc gaagagccgc gtggagccac
     6001 cgatcagtgc ttgcagggga gcgctcatcg tcattcccct gcaattgaca aagccgccgc
     6061 tgcgagcggg gtcgagcacg cccggggcgt gcagccagca agatgcgccg ccagcgcgat
     6121 cggctcaggc gggaacaggc gtgcacctat cgtgtcgaag gtcggcagct cgggaacggc
     6181 atggccattc cccgatccgc cgatcagcga tgtatcgccc gagcggatca gctcgataaa
     6241 ttcctcagcg gtgacgcgct gcattcaaat ctccaatgat catttcagtt gcccggcaaa
     6301 atggcaggcg gccaaatggc cgggctcatg ctcgaccaaa gggggcggct cgctggcgca
     6361 gatcggcttg gccaaggggc agcgggtgcg gaatcggcag ccgctggggg gcgagatcga
     6421 gcttggcggc tcgccctcga gcttcagata gccgcgctcg cgctcgaccg ccgcatccgg
     6481 gatcggcgcg gccgaaagca ggtagcgggt gtaggggtgc agtggccgat cgaacagccg
     6541 atcagccggg gcgatctcca tcaccttgcc aaggtaaagg accaccacgc ggtccgagat
     6601 atggcgcacc accgcgaggt cgtgagcgat gaacaggtag gtcagcccga acctttcctg
     6661 caaatcgagc atcaagttga tgatctgggc ctgaatattg acgtcaagcg ccgagatcgg
     6721 ctcgtcggcg acgatgaaat ccggcctcac ggcgagggcg cgggcgatcg aaacgcgctg
     6781 gcgctggccg ccagagaact cgtacgaata gcgccgcccg acgcgcgggt tgaggccaac
     6841 gatgttgagt agctcgtcga cacgctcctg cgtctccgcc ttgcctttcg ttatcccgtg
     6901 aaacagcagt ggctcggcga ttatgtcacc aaccgtcatg cgcgggttga gcgaaccata
     6961 gggatcctgg aagaccatct gcatgcgccg gcgcagcggc ctgatctgct tctgcgtgct
     7021 cttcgcaata tcgtggccgt cgaaaatgat ctcgccggaa tccggctggt ggatgcgcat
     7081 caccaaacgg gcaatagtcg acttcccgca gcctgactcg ccgacgagcc cgaccgtctc
     7141 gccgcggaag acatcgagat cgacgttgtc gacggcgtgg acgacgcgcc gcggctggaa
     7201 gtagccggtc ttgggcagtg ggaaatgctt gacgacgtca cgcagcttca acaggggcgc
     7261 ctcatcgcgg ccagcaatga cgcgcggcgg agtcgcctcc gccgaagcgg ctgtggcccg
     7321 cagcgtcttc ttcgggagcg tagtgccggc ctgcgtcacc cagcagcgcg ccttacggcc
     7381 gggcgcgacc tcggcgaggt ccggatgcac atcgcatttc tccacccgga aggggcaacg
     7441 cggcgcgaaa cggcagccct gaggcaggtt aagaggatcc ggtggctgac cttcgatcgt
     7501 ggtcaggcga cggtcgaggg gccgtgcctc gtcgatgcgc ggcactgcgt tgatcagcgc
     7561 ccaggtataa ggatggcgcg gatcggcgag cagatcctcc gtcaacccct cctcgaccac
     7621 ctcgccgcta tacatgacga tcgtgcggtg gcagacgcgg gcgataacac cgagatcgtg
     7681 gctgatcaga acgacggagg tgccgtaatc ccgattcaac tcgcggatca ggtcgaggat
     7741 ctgcgcctgg atggtgacgt cgagtgccgt cgtcggctca tcggcgatca acagggccgg
     7801 ctcgttgctc atgccgagcg ccagcatgac gcgctggcgc atgccgcccg agaactggtg
     7861 cggatagctt tcgacggcgc gctcgggcgc ggtcacgccc atgcggccaa gcaggtcgac
     7921 ggcacgcttg gaggcttccg cctctgtgaa gcggccgtgc accaccatgg tctcgatcaa
     7981 ctgcttggca atcttcagca ccgggttgag cgaggtcatg gggtcctgaa agaccatcgc
     8041 catgcggtcg ccgcgcaccg agcgtagctc ctcggggctc atcgccagca cgtctctgcc
     8101 ctgaaagcgc acctcgccct cggtgatacg ggctggctca tcgagtagcc gcattagcga
     8161 cagggcagta acgctcttgc cggagccgga ttcgccaacg atgccgaggg cctcgccctg
     8221 tttcacggtg aaggacacac cgtcaaccgc cgagacgacg ccgtcgtcgg tgaaaaactg
     8281 cgtgtgcaga tttttcacct cgagcgcggg ggcatcggca ccggtcgcaa cgcggccgag
     8341 ttcggccttg agggtcttgg tcatggcgcc cctaacgttt cctggccttg gggtcgagcc
     8401 agtcgcgcac gccgtcacca aggaagttga agcccagcac gatgctcagg atgcagaagc
     8461 cggggaaggt cgcgacccac cattgagcga ccagctcgcg cccctccgcc accatcgagc
     8521 cccactcggc cgtgggcggc acgacgccga gcccgagaaa ggacagccct gcaaaggtga
     8581 tgacggcgtt accgatatcg agcgtcacga gcacgatcag cggcccgatc gagttcggga
     8641 tgatgtggcg aaacaggatg cgaccggggc cgaaaccggc gacgaccgcc gcctcgacat
     8701 attcctgcga gcgctgcgtc aacaccatgg aatgcgcgag gcgggcgaat ttcggccacc
     8761 acaccgccag catggcgatg atggtgttga cggtgccgat gccgagcgcg gcggcaatgg
     8821 cgagcgccag gatgatcggc ggaaagcata ggaccagatc ggtcaggcgc atgatgattt
     8881 cttcgacctt gccgcgggcg aaggcggcga tgcccccgag cagcgtgccg aagaatgctc
     8941 cgaccagaac caccacgaag cccgtggtca gcgaaatgcg cgacccgtac agcaatctcg
     9001 tgaacacgtc gcgcccgagc gcgtcggtgc ccagccaatg ttcgccagag ggcggcttca
     9061 gccggttgga aacatcgacc agattcggat cgtacgggct gagcaatggc gcggtgatcg
     9121 caactacgat ccaaaccagg atgatgaaca tcccgactgc cggcaggccg taccgcctgc
     9181 gccagcgctg cgccttggtc gggactggcg cgatgcgccg tgccgctgct gcctcacccg
     9241 caacgctcac cgcgtcaccc gtgggtcgag gaaggcatag ctgatgtcga tcaccaggtt
     9301 gatgatcaga tagaccagcg ctaccaccag cgtgatgccc atgatggccg gaaaatccag
     9361 cgccgcggca ctgcggaagg tatagaggcc aagacccggc caggagaaga tcgtctctgt
     9421 catcactgcg ccactgagca aattggcata ggcgaggccg ccgagcgtga ccaccgggat
     9481 cacagcgttc ggaaatacgt ggcccagaat gatcgtgctc tttttcaacc ccttggcgcg
     9541 ggcgacgcgc acgtaatcct gctggatgct ctccaacatg ctggcgcgcg tcgtgcgggt
     9601 gatcaggccg agcgtcgctg cagcctgcac gatcgacggc aacaccagat gcgccgcagc
     9661 atcgcggaag gtctcccagt cgccggcgat ggccgtgtcg atcagcagca agccggtgac
     9721 ccgctccggc ggtagcgcga tcggatcgag ccggccggga ccgggagcga tctcaagccc
     9781 accatagaag atggcgagca taatgaacgc gagccagaaa gtcggcgacg acacgccgat
     9841 cagcgacacc acacgcgcga tatggtcgac ccaggtgtcg cgccagacgg cggcgatgat
     9901 gccgagcgga atgccgatca gcagagcaag gacgaagccg attgtggcaa gctcgatcgt
     9961 cgcgggtgca aactgtatga tgtcatcgag caccggacgt tgcgaggcga tcgagatgcc
    10021 gagatcgcca tggaccaggc cggaaaggaa aatcaggtaa cgctgccaga gcggcaggtc
    10081 gagaccgaat ttggtccgcc actcggcgac gaatttttcg tcggaggcgg cgacgtcgcc
    10141 gagctgcgcc agcacgggat cacccggcac agtgtttgca atcaggaaga tcaccagcgt
    10201 ggcgagcacc gccatcacga ccgtaatgcc cagccgcgag acgatatagc gcgtcattcc
    10261 cctgtctccc ggctgacgag cggccgccgg ggcaagattt tcaggagatt ggtcacctcc
    10321 atgcttcctc ccgaattagc gccatttttt tcaacttaga tcgttctaaa cttcacatgc
    10381 cgggctgtca acaagtgcct aggggtccac gatatcatcg gctttgcctc aaagatccta
    10441 atttttgttc agcctcgaaa gccgctggcg ggcccttgtc gcgctcagcg cgggcgcgga
    10501 cagctggatt cacggatgat cagggaaggt tgcgaaatca ctcgtttcgg aggtgcgtcc
    10561 ggattctcga tacgctcgag caacagcttc gcagcggccg tgccgatggc atgcgtgtcg
    10621 atgcgcactg tcgacagggc cggcgtccac agctccgcct cggcgagatc gtcgtagccg
    10681 aacaccgcaa agtcgcgccc cggctcccgg ccgatctggc gcaggccaag catgactccg
    10741 aaggcgatca cgtcgttgaa acacaccgcc gccgtcggcg gatcgtcaag acgcatcagc
    10801 cgctttatgg cctcggcgcc gaagctgcgg ctcggcgtgc cggcgaggac cagttcgggc
    10861 gccaggggca ggccgttggc tgcgagcgcg tcacgatagc cgaggcgccg ctcgcgaccg
    10921 gtccagttgc ggtcagcccc gccgatcatg gcgatacgcc ggtggccgag accgatgagg
    10981 tgctcggtta ccatcgaggt gccctgacgg tgggcatggc cagcataatc gaggccgctg
    11041 ccggggacat cgcgcgacag cagcacgcac gcgatgcctt gctccttcaa gcgccgaagg
    11101 ctctccgcct cggtgccctc ggccgggcag atcaccaacc cgtcggcgtt atactcgcgc
    11161 attgtcgcga tgaactggtc ctgccgggca gggtcctcat ccgaattcga cacgaacacc
    11221 gtacggccga gatcacggaa ggccccctgc agtacggcag tcagctcggt aaagtatgga
    11281 ttggtgatct cgttgatggc gatgccgatc gtatgggtac gccgtgtgcg cagattggcg
    11341 gcgccgcgat tatagacgta gccgaggaca cgcaccgatt cgagcacgcg ggcgcgcgtc
    11401 ttctcggcca cgagaggact atttgccagt accagcgaga cagtggcccg cgatacgccg
    11461 gcatgagcgg cgagatcctg cagcgtcagc cgctcccggg ggcgcaccgg tttcggccgg
    11521 tcatccatgt cgtcgaagtc cccaaatcgt gacgaacttt gatggagaat acagcatgtc
    11581 tgatgagatc cgcaccgtga agggtgattt cattgccgag atcatcctcg accgtcccag
    11641 caagatgaat gcgatcacgc ctgccatggc ggccgctatc gagcaccact gccgagagtt
    11701 cgatcgcgac gacagcgttc gcgtggtgct gttgcgcggc gccggtgatc gcgccttttg
    11761 cgccggtagc gacctcaacg ctctggcaga gtatccgacg ccgtggaaat tccgcaaccg
    11821 ggtggaatat gccacagccg tgcgcaacat gcgcaagccg gtcgtggcgg cgctgcatgg
    11881 ctgggtgctc ggcggcggcg ccgagatggc actttcagcc gacatccgcg taatgggccg
    11941 cagcgcccgc ctcggcttcc cggaggtcaa gcgcggctgg gtcggcggcg gcggtgcctc
    12001 gcagatgctg ccgcgcctga tcggctacgg ccaggcgatg cgcctgctgc ttctcggaga
    12061 gccgcttgat gcggaagagg cgatgcggct cggcatcgtc gagttcctgg tggacgatgc
    12121 cgaggtcgaa gccaaagccc gcgacatctg ccgtactatt gccagctata gcccagtcgc
    12181 ggtggaatcg gtcaaggccg cggtgcgcat ggcgctgcaa tccgacctgg cgagcggcat
    12241 tcgctacgag aacgagatga acacgctgtg cttctccgcc ggcgaccaca tggaaggcat
    12301 tcgggccttt caggaaaagc gcgaggcggt cttcaaggcg taacagcagg catcaagcat
    12361 tatgcccgct gtggaaagcg ccgcccccga agcctcgccc gctacaccgg cattggcgca
    12421 ccggaggcgg cgcacaatac gcgggttgtg ctcagcatga cctagtgtcc cgattcagaa
    12481 gttcctatcc aggcggtacg tgaacttgga gtgtgcggcg gcagaagcgc tcgatggagg
    12541 caaggatatc gtcggcggat ttggtccagc gcagcggcct cgggtcggcg ttctggcgct
    12601 cgatgaacgc ctcgatgtcc ttgatgagcg ctgccaccga tctgtgcgca ccgcgcttga
    12661 tctgcttctc ggcgagcagg gcgaagaagc gctccacctg attgatccag gacgacgagg
    12721 tcggcgtgaa gtgcatgtgc catcggggtc gcttggcgaa ccagtcgcgg atcagcttcg
    12781 tcttgtggct cgacgcgttg tccatcacga catggatgtc gaacctgtcg gggacgttcc
    12841 tctcgatctc gtcgagaaac gtgcggaact cccgcgcccg gtgccgcggc atgcacttgc
    12901 cgatgacggt gccggctttc acgtcggcct tggccgtgac cgacgcgatg aggcccgcga
    12961 agagcgtcgt cgttccgtga cgctcgtagt cgtgcgtgcg ccgctcgatc tgtccagggc
    13021 gcaacggcag cagcggttgc gtacgatcga gcgcctggat ctggctcttt tcgtcgacgc
    13081 aaatgactag cgccttctcc ggcggggcga gatacaggcc gacgatgtcg cgcaccttct
    13141 cgacgaactg cggatcggcc gacagcttga acgtttcgct ccgatgcggc tgcagcgaga
    13201 acgcccgcca gatccgatgc accgtcgatg tcgagacgcc cgacgctttg gccatgccgc
    13261 gcgtgctcca atgcgtcgcg ttcgccggct tctcttcgag cgtcttgacc acgatggctt
    13321 cgatgcggtc gtcgccgatc ttgcgcggtg cgccgcatct cggctcgtcg ccgagaccgt
    13381 ccagccggtc cttggcgaag cgctcacgcc acgtgcggac cgtctggcgc gtgaccccca
    13441 cggcgtccgc gatctggcag ttgttctccc cgtcggcggc ccgcaagatg atctcggcgc
    13501 gctgcgcttc ggcccgggcg atcttgcggc gccggatacg gcgctcaaga acgtcccgct
    13561 catcggccga aagttcgatc ggacttgcca cccgcctcgt catgcgatcc cccgattcgc
    13621 catggcacaa atcaggaatc acacgcacga tctcatggct aggaattaat gagacgggac
    13681 actagatggc aatcggcacc caggccaacg gcggcaggag acgcagcagg ctgatcagtg
    13741 gctgcggata ctagcgcacg gtcgtcgcca gcccatcaga atgccgagtg gccgacggag
    13801 cagcagcgcc agtccaaatg aggcgtatgc ccgaaaagca cgctgtccac atcgacgcag
    13861 tctcgcaaaa aagaaccgga tatccttctt ctggttatga tctcggatgg cgctttagcc
    13921 gcgagccctg ttccaaaccc agcgtacgaa ggggaagagc cacaccgcca gagtgattgc
    13981 cccgagaatg ccggccatcg gccgttcgaa aaggccgacg atgctcccct gcgtactgat
    14041 caaggactgc atcaagtgct gctcgaccag tttcccgagc acgataccga gcaccatcgg
    14101 tgcgagtgga aacttgttct cctccaag
//
LOCUS       DASVDU010000063        22232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1468053, whole genome shotgun sequence.
ACCESSION   DASVDU010000063 DASVDU010000000
VERSION     DASVDU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22232
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468053"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VF226_04250"
     CDS             complement(<1..906)
                     /locus_tag="VF226_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7073229.1"
                     /translation="MTNPEVGMLMLALFIFIILLGFPIAFTLIAMGVFFGYFAMGTRI
                     FDLLVTNTADVMQNDVLTAVPLFLFMGYVIERSNILDRLFHSIQMAARNLPGSLAIAT
                     LITCALFATATGIVGAVVTLMGLLAFPAMLRAGYDTRLAAGVVCAGGCLGIMIPPSVL
                     LILYGATTATSVVKLYAAAFLPGFLLASLYVLYVIARAVINPKLAPKPPDLEEHIPFI
                     KVISALMTSFFPLFILIMAVLGAILFGLATPTEAAAVGCAGALLLAALYRALTFERLK
                     EATYLTARTSAMVCYLFIGSWTFSSV"
     gene            complement(903..1553)
                     /locus_tag="VF226_04255"
     CDS             complement(903..1553)
                     /locus_tag="VF226_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7073230.1"
                     /translation="MTCAQNNRTGEGTHVVGFTLLIDRLSAGVGKAFGWCMIILTFGT
                     CYEVFMRYVLRAPTNWAFDMAYTMYGALFLMAGAYTLSRNGHVRGDVVYRLLPVRMQA
                     AIDLALYVLFFFPGFVALLVYGYPYAMTSFMIREVSIYSPAGQPIWPLKALIPIGAVF
                     MLIQGVAESIRCIVALRDGVWPQRLQDVEEMESAILADEEARARLAQQQAAGGAAQ"
     gene            complement(1601..2734)
                     /locus_tag="VF226_04260"
     CDS             complement(1601..2734)
                     /locus_tag="VF226_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073231.1"
                     /translation="MSEKKPAMTTRRKFLAGAAVTGAATIAMPQVSRAETVTLKMQGS
                     WGAQDMLNEMAQDFVKIVNELGEGRLKVDYLNAGAVVKPFSVMDGCHSGVLDGAHSVP
                     AYWYGKHRAASLFGTGPAYGFNGNQLLGWVYYGGGLELYNELIQDVLKLNVVGFLGFA
                     MPTQPLGWFKNEVSGPEDLKGLKYRTVGLASNLFQAMGLSVAQLPGPEILPAMDRGVI
                     EAFEYNNPTSDLRFGAQDVSKLYYMGSYHQAAETFEILFNKTKFESLAKEQQAILKHA
                     VQAAAADNEWKAWHAYSEDLQKLINDHGVKVKRTPKSIFEAQLKAWDEVEAELVKDPQ
                     QGPFIKKVLDSQKAWAKRVGFYHFNNDADYRTAYEHHFGPIET"
     gene            3076..3963
                     /locus_tag="VF226_04265"
     CDS             3076..3963
                     /locus_tag="VF226_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7073232.1"
                     /translation="MNIVDRSLYLSGGRTGILLIHGLGGTPIEMRFIAVGLARAGYTV
                     SCPQLAGHCGTADDLRATRWQDWYASVCAAHDRLRQECDVVIVGGLSMGAVMALRLAA
                     DRPDSVHAAALFAPTFRLDGWSVPWYARLFRLVWHRWVADRFDFVEREPYGIKDPRVR
                     GLVTAALQSGDSSQAGVLSTPGSSVLELRRLANSVRRSLRNIRQPVLVLHPREDDHAS
                     LGNAFDLQKKLGGRVEAVVLDDCYHIITLDRQRHLVVERTTRFVREVCQQLQEKAAAV
                     KVGPAQVSPIRADQPLPAA"
     gene            4079..4630
                     /locus_tag="VF226_04270"
     CDS             4079..4630
                     /locus_tag="VF226_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073233.1"
                     /translation="MNLKYANGKIIALATVVLAGLSLAAPGAQAGMRIHFGFPIGSFY
                     AGGHHHHHHAHRYEYRRLQALRRARAIEAARRRQAAAEAAAARAERKRAAALAAAAAK
                     RQAAAELAAAEQKKLEAAREAELAAVPLPERKPETPAGEALQTADATTAANSQPATET
                     PAKLECKRYFANVGMTIRVPCSE"
     gene            complement(4720..5334)
                     /locus_tag="VF226_04275"
     CDS             complement(4720..5334)
                     /locus_tag="VF226_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX7073234.1"
                     /translation="MTVKRRSQHLAGSSYLEGQLLIAMPAMADKRFARSVIYMCAHSD
                     EGAMGLIVNQRAPNLSFPDLLERLQLLDEDAEISVPSKMLHMPVHLGGPVEPERGFVL
                     HSSDYFTPSSTLPIDDNVCLTATIDILKALAAGEGPDRAILALGYAGWAPGQLESEIH
                     ANGWLSCQADLELVFDLDVEEKYERALYKLGIDPTHLVNAAGHA"
     gene            5490..6347
                     /locus_tag="VF226_04280"
     CDS             5490..6347
                     /locus_tag="VF226_04280"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX7073235.1"
                     /translation="MHGCRVHLLASALAILLAFSTPAAADPLASPWVEAHGGKARLIA
                     GTVDSAGRSAPKLLAGIEMRLAPGWKTYWRSPGDSGGLPPQFDWSQSANLDVAKVLYP
                     APSRFKDALGDALGYSGSVIFPVELRPKNSSKPIELRLSLLYGICHEICIPAEAKLTL
                     QIPATATIPADPGISAALSAVPRSSDERRPNDPELISKAARLDGDRPRLILEARFPKG
                     ASSGDMFIEVLEGGFVPLPVKTQTRGDTARFEADLTQGAEPDELRGKTLRITMVSDAG
                     QSEALWKLE"
     gene            6464..6952
                     /locus_tag="VF226_04285"
     CDS             6464..6952
                     /locus_tag="VF226_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7073236.1"
                     /translation="MTINVGDRLPEATFTVMGPEGPKQVTTGEVFGGKKVALFAVPGA
                     YTPTCHKQHMPGFVANAPEFQNKGIDMIACTAVNDIFVLTQWAKDSGAEGKITMLADG
                     SAEFARKIGLDIDLSARGLGVRSKRYSMIVDDGVVKVLNVEDAPPQHDKSSAVNLLAC
                     SF"
     gene            complement(7033..7728)
                     /locus_tag="VF226_04290"
     CDS             complement(7033..7728)
                     /locus_tag="VF226_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073237.1"
                     /translation="MSLSLAGVHAYYGNFHVLQNISMVVEQGTIHVLLGRNGAGKTTT
                     LRTIFGLMDKWSGSITLDGQSIGGRPPHLITRAGLSYVPEYRGVFSGLTVRENLAISE
                     YPGSPWPAERVLDLFPPLKPLLGRMGGHLSGGEQQMVAIGRALMSGPKFLLLDEPSQG
                     LAPVIVDIVVDTLLRLKEENLGILLVEQNAEIALEIGDEATIIEQGEIVHRGTAAETR
                     ANPDVMTTYLSVG"
     gene            complement(7725..8474)
                     /locus_tag="VF226_04295"
     CDS             complement(7725..8474)
                     /locus_tag="VF226_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073238.1"
                     /translation="MTTGLECISVSKHFGAVKAVNDVSFEVPPGALHTVIGPNGAGKT
                     TLFNCITSELKPTSGEVRFEGHVISGRKTHELPAFGVARSFQRTSIFSNLTVAQNVWV
                     SAFRAFAGTRVEVLRPADAHPEVTETIYQTLEEVGLDRYANSPATELAHGDQRLLDFA
                     IALAPKPKLLLLDEPTAGLSRSDTQRVMHLIESLKGRYTIVLIEHKMDVVMRISDRIT
                     VMYFGEVLAEGTPQEIRANPKVREAYLGRRA"
     gene            complement(8471..9430)
                     /locus_tag="VF226_04300"
     CDS             complement(8471..9430)
                     /locus_tag="VF226_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073239.1"
                     /translation="MQKLFSLPSLIVIALLIVGLPFLQRPVVAAEVLTLGIAAVATNL
                     LLGYTGMLSFGQALFFGVGAYCAGILATRYGISAFLAIPVAMLFSAVIAFLIGLVCVR
                     RSGLYFIMITFAFNQMVFFIAYSWTTMTGGEDGLPGVRRPPLIDDQWSFYVFTVLVFI
                     CALCVMKLFVDSPTGRILQAIRDNPGRAAATGHDVGRYKLIAFVMAGMFTGLAGAIFA
                     FVFGIVPIDKVDWRFSGDIVFMTLLGGSGSFIGPVIGAAFYTWLQETVSHYWDRWPMI
                     LGIVFALVVLFFRTGVAGMAEFLTGKLTQKGAAAKPKEMEAGS"
     gene            complement(9431..10291)
                     /locus_tag="VF226_04305"
     CDS             complement(9431..10291)
                     /locus_tag="VF226_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073240.1"
                     /translation="MTFLIASQLLNGLATGMLYALVGVGLTLVLGMLNIPNFAHGGLY
                     AFGAYFTYTVVNLTGSFYLAVLIGPLLVILVAFLIESQGVRRLYAAHHDYQLLFLFAM
                     ALILQEAIILIWGPVGFSVLTPKALSGVVNLGFTVYPAYRLALIGMTVLITVALWLFL
                     TRTNVGAIVRAGMENREMVAVLGTDINRVFMWTFALGAYLAGLAGALATPIMGLTPSM
                     GADMLPISFVIVVIAGLGSILGAIASGILIGVAQSLTTIWWPEASNIAIYVLMGLTII
                     LRPQGLFGTR"
     gene            complement(10382..11602)
                     /locus_tag="VF226_04310"
     CDS             complement(10382..11602)
                     /locus_tag="VF226_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073241.1"
                     /translation="MKRVSLGLKGMAAAATIALAATAMTANAADDEIVLLGLWPFSGP
                     YADVGPLLDHGAQVALEQFGYQAAGKKIKYITRDSETKAGTATRRAQEAIDGEGVKFM
                     VGPWSSGVGLAVSDVAQKNKVMYAFSGGTEDLSGKRCHRYTFQWAAPAYTAVEANLRV
                     FKKNHPEAKSLYLFIADYAFGWSLQKYAEMLAPKFGLTIAGVDRHPLGHREFSSYITK
                     AVAANPDAIMMVNFGIDAVTALRQIYNFGFTPKKPVIMAWSSGTEELIQLDPEMRENL
                     QVGTNYYWTIDNPENKAFVEAYRKKSGGVPPGYAPAAAYGMTHAILTGIQLANSAEPQ
                     KAIEALENFKGKTILGETSVNPKTHQINRPYFILKTKSKSEMKDEYDLATVIDESSIE
                     QPPELNECKDIGGF"
     gene            complement(11686..12393)
                     /locus_tag="VF226_04315"
     CDS             complement(11686..12393)
                     /locus_tag="VF226_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018001030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7073242.1"
                     /translation="MDLGKLDEMLQRAFDESTKIYQQRGFQRRIGFGRKPALVSVDLA
                     NAWTRPGNPFTCEKIDEEIIPGMQRLLEACRKNGHVVVHVTTAYQNTNRNDRTSDMGL
                     WHYKIPVEAVDIADEHLWAIDSRIAPIEGEYVMLKKRASAFSGTPLAGYLRSAGVDTI
                     LVTGVTATACVRTTICDGLAEGFRTIAVRECIGDRVPGAVAWNLFDIDAKFADVESVD
                     TCVNYLNNVEGFNQTHG"
     gene            complement(12573..13025)
                     /gene="rnhA"
                     /locus_tag="VF226_04320"
     CDS             complement(12573..13025)
                     /gene="rnhA"
                     /locus_tag="VF226_04320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEX7073243.1"
                     /translation="MTSRRPVVEIYTDGACSGNPGPGGWGAILISGPHRKELWGGDPV
                     TTNNRMELMAAIRALEALKRPSIVDLYTDSAYLRNGITAWIHSWKRNNWRTADKKPVK
                     NAELWQRLDEALKPHEIRWHWVKGHAGHPENERADELARKAIREIRGS"
     gene            complement(13022..13993)
                     /locus_tag="VF226_04325"
     CDS             complement(13022..13993)
                     /locus_tag="VF226_04325"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7073244.1"
                     /translation="MAVYTEVADDELARFIERYGLGELLSCKGIAEGVENTNYLVQTA
                     KGRFILTLYEKRVQREDLPFFLGLMEHLAKAGITCPTPVRDRDGVMLNELAGRPAALV
                     TFLDGMWIRRPRPEHCHAVGEALAKLHLAGRDYKLTRENALGPKGWEALFERFEDRAD
                     EVAPGLGDEIRRELEELLPAWPQGLPCGVIHADLFPDNVFFRGTELSGLIDFYFACND
                     FFAYDIAVCLNAWCFEADFAFNITKSRALLKGYTAVRELEPAEHEALPILARGAALRF
                     LLTRAYDWLHTSQNALVKPHNPLEYRRRLRFHRTVRSVGEYGLETAQ"
     gene            complement(13998..14963)
                     /gene="ispH"
                     /locus_tag="VF226_04330"
                     /gene_synonym="lytB"
     CDS             complement(13998..14963)
                     /gene="ispH"
                     /locus_tag="VF226_04330"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7073245.1"
                     /translation="MTLILAAPRGFCAGVDRAIQIVELALAKYGPPVYVRHEIVHNRF
                     VVDSLRAKGAIFVEELDEVPETDAPVIFSAHGVPKWVPAAAKARNLFHLDATCPLVSK
                     VHKEAHQHYAHGRELVLIGHAGHPEIIGTMGQLPEGAVTLIETVEQARAFTPRDPQKL
                     AYATQTTLSVDDTAEIVAVLKERFPHIVGPHKEDICYATTNRQAAVKSIAPRVERFLV
                     VGAPNSSNSLRLVEVAERAGCQRAMLVQRAADVDWAAFKGVRTVGLTAGASAPEELVN
                     EIVDAFRERFDVTVETVSTAQEHIAFNVPRELRDFAPARKLSASA"
     gene            15297..15866
                     /locus_tag="VF226_04335"
     CDS             15297..15866
                     /locus_tag="VF226_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073246.1"
                     /translation="MSSLTSGIGGGMLGGSSSSSTEVASVSEEQLLSAAKADYGTTGS
                     SGIDLAHGCPRFVIWPRDNSVTIYEQGRVGDALAVMHRGEITKTARECHIENGRVTVK
                     YGFSGRVLLGPRGKPGSVKLPVNVFVTDARREKIAQDTMAVDVDVALERPIGYFSVVR
                     TVSFTIPEGTRPAEYEVFVGFDRNLPNAG"
     regulatory      15969..16064
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            16243..17388
                     /gene="gcvT"
                     /locus_tag="VF226_04340"
     CDS             16243..17388
                     /gene="gcvT"
                     /locus_tag="VF226_04340"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7073247.1"
                     /translation="MSADAANEALKRTPLYDLHVELGAKMVPFAGYEMPVQYQLGILK
                     EHQHTRARAGLFDVSHMGQAKLVGPDHQTTAAALEALVPADILNLKPGMQRYTQLLNP
                     QGGIVDDLMVARPADPAEDGTLFLVVNASRKEVDYEYISAHLPDTVRLEPQPDRALLA
                     LQGPEAAATLSKHSPEAVTLGFMGVRRVPVGPFECHVSRSGYTGEDGFEISVAADQAV
                     DLARLLLAEEAVQPIGLGARDSLRLEAGLCLYGHELDEMISPVEAGLAWSIQKRRREG
                     GGFPGAERIQKELREGPARLRVGIKPEGRAPARDGTEILEPSGARIGIITSGGFGPTV
                     NGPIAMGYVESSFAKPGTAVQLMVRGKPQPAHIVELPFVPHRYLRKA"
     gene            17406..17780
                     /gene="gcvH"
                     /locus_tag="VF226_04345"
     CDS             17406..17780
                     /gene="gcvH"
                     /locus_tag="VF226_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7073248.1"
                     /translation="MALERFTEDHEYIRVDGDIGTVGITVHAQEQLGDVVFVEVPEVG
                     RTVKQKESTAVVESVKAASEIYAPVSGEVVEVNTELAANPALVNEDAEGKAWFFKLRI
                     ADSSELSSLMDRAAYEEFLKQS"
     gene            17926..19269
                     /gene="gcvPA"
                     /locus_tag="VF226_04350"
     CDS             17926..19269
                     /gene="gcvPA"
                     /locus_tag="VF226_04350"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX7073249.1"
                     /translation="MRYLPLEQADREAMLARIGVPAVDALFASVPADKLMKELPDLPR
                     TKGELEVERELSKMAARNVPAGSVPFFVGTGAYRHHVPATVDHLIQRSEFLTSYTPYQ
                     PEISQGTLQTLFEFQTQVALLTGMEVANASMYDGSTACGEAVLMANRVTRRKKAVLSG
                     GLHPHYRSVAETLSRWGGSDVVALDPDPTGAEDIISQIDDQTSCVVVQYPNAYGTIRD
                     LAPIAEAAHAQGALLIAVVTEIVSLGLLKPPGEMGADIVAAEGQSIGNGLNFGGPYVG
                     LFATREKFVRQMPGRLAGETVDAEGRRGFVLTLSTREQHIRREKATSNICTNSGLCAL
                     AFTIHMTLLGEAGLRRLAQINHANAVTLADQLSRIGGIEVITPAFFNEFTIRVPGDAQ
                     QIIDALAKKGVMGGVPVSRLEPSRNDLRDLIIVASTEINTDEDRAAYVKALQEVL"
     gene            19269..20849
                     /gene="gcvPB"
                     /locus_tag="VF226_04355"
     CDS             19269..20849
                     /gene="gcvPB"
                     /locus_tag="VF226_04355"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7073250.1"
                     /translation="MAMNSQGRPTTPGAAGPAASGIETFTGHRGLDHEELLIFESGSL
                     SKSGVDLPEPKPVKSRLNGLERNGPIGLPGLTEPEAMRHYVRLSQRNYAIDTGLYPLG
                     SCTMKHNPRLNEKMARLPGFGDVHPLQPQQTVQGAIELMEALSGYLLELTGMKAVALS
                     PKAGAHGELCGMLAIKAAHEAAGSKRSVVLVPESAHGTNPATAAFMGYTVRSIPARED
                     GTVSADAVREALGPDVAAIMLTNPNTCGLFEPEVVEIAKAVHDAGAYFYCDGANFNAI
                     VGKVRPGELGVDAMHINLHKTFSTPHGGGGPGSGPVVLSDALAKFAPVPFVVRDGDEL
                     RLIEDISEAEARGQKPFGRLTAFHGQMGMFVRALTYMMSHGADGMRQASEDAVLAANY
                     VRVGLKDLFSQPFGDQPCMHEVLFDDKFLKDTGVTTLDFAKAMIDEGYHPMTVYFPLV
                     VHGAMLIEPTESESKDNLDLFIATMRDLAEAAKRGDVDRFKQAPRLAPRRRLDETRAA
                     REPKLRWAPDAARKQAAE"
     gene            21060..21554
                     /locus_tag="VF226_04360"
     CDS             21060..21554
                     /locus_tag="VF226_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7073251.1"
                     /translation="MTDVREVFVSGLRDAYAMEKQAKDMMESQASRLEDYPEMQSRAA
                     QHARETDAQIDRLRQCLEMIGESPSMFKEFGTRAMANLQSMLSAASTDEPIKETLTGF
                     AFENFEIASYRSLIAFAKQLGEQRMVPLLEESLREEERMANWVGDHIESITEQFASRA
                     SATA"
     gene            complement(21614..>22232)
                     /gene="acnA"
                     /locus_tag="VF226_04365"
     CDS             complement(21614..>22232)
                     /gene="acnA"
                     /locus_tag="VF226_04365"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505001.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX7073252.1"
                     /translation="TIYDASQRYIAQGTPLIILAGKEYGSGSSRDWAAKGPHLQGVKA
                     VIAESFERIHRSNLVGMGILPLQFIPGQSVESLGLTGKETFAIEGITEGVANGFANTR
                     NLTVRVQREDGSEQTFEAIARIDTPQEVLYYRHGGILQFVLRQLLTGKEELPVAGKAP
                     ARISEEQDAVADDLYVEEGSIESFPASDPPAHSAGRAVRGAPPKT"
BASE COUNT         4274 a         6999 c         6918 g         4041 t
ORIGIN      
        1 gaccgaagag aaggtccacg atccgatgaa cagatagcag accatggcgg aagtccgcgc
       61 cgtaagatag gtcgcttcct tcagccgctc gaacgtcagc gcgcgataaa gcgccgccag
      121 cagcagtgca cctgcacacc cgacggctgc agcttcggtc ggtgtggcaa gcccgaaaag
      181 aattgcgccg agcactgcca taatcaggat gaagagcgga aagaatgagg tcatcagcgc
      241 cgaaatgacc ttgatgaaag gtatgtgctc ttccagatcg ggcggcttag gtgccagctt
      301 cggattaatg actgcacgcg ctatgacata cagaacatag agactggcaa gcagaaaacc
      361 gggcagaaaa gcagccgcat agagcttcac cactgaggtg gccgtcgtcg caccgtatag
      421 gatgagcaga acgctgggcg ggatcataat gccgagacac ccgccagcac agaccacgcc
      481 ggctgcgagc cgggtgtcat agcccgcgcg cagcatggct gggaacgcca gcaatcccat
      541 cagcgtgacg acggcgccaa cgatacccgt tgccgtggcg aacaaggcgc atgtgatgag
      601 cgttgcaatc gcgagcgacc ccggcaggtt tctggctgcc atttgaatcg agtgaaacaa
      661 tcggtcgaga atattggacc gctcgatcac gtatcccatg aagaggaaca atggcaccgc
      721 cgtcaggaca tcgttctgca tcacgtcggc agtattggtg accagcagat cgaagatccg
      781 ggtccccatc gcgaaatagc cgaagaagac gcccatcgcg atgagcgtga aggcgattgg
      841 aaagcccagc aggatgatga aaataaacag cgccagcatg agcatgccga cttcgggatt
      901 cgtcattggg cagctccacc ggcagcttgc tgctgtgcaa ggcgcgcgcg cgcctcttca
      961 tcggcgagaa ttgcggactc catctcttcc acgtcctgaa gccgttgcgg ccagactcca
     1021 tcgcgcaatg caacgataca gcgaatcgat tccgcaacgc cctggatgag catgaacacg
     1081 gcgccgatgg gaataagcgc cttcagcggc caaatgggtt ggccggcggg gctatagatc
     1141 gagacctctc gtatcatgaa ggatgtcatt gcgtagggat atccgtagac aagcagcgcc
     1201 acgaatcccg ggaaaaagaa gagaacgtat agggcgagat cgatcgctgc ctgcatcctg
     1261 actggaagca gccgatagac gacatcaccg cgcacgtggc cgttccgcga tagcgtgtat
     1321 gctcctgcca tcaggaacaa cgccccgtac atggtgtagg ccatgtcgaa cgcccagttc
     1381 gtcggagccc ggaggacata gcgcatgaaa acttcgtagc aggtgccgaa ggtcagaatg
     1441 atcatgcacc agccaaaagc cttaccgacg cccgcgctga ggcggtcgat aaggagagta
     1501 aagccgacca cgtgggtacc ctcgcctgtt cgattgtttt gggcacacgt catcggccgg
     1561 gccttctatc gagcccggcc gacaactcac tggagtgcca tcaggtctcg attggaccga
     1621 agtgatgctc gtaagccgtc ctgtaatcgg cgtcgttgtt gaagtgatag aagccgaccc
     1681 gcttggccca ggccttctgg ctgtcgagca ctttcttgat gaacggcccc tgctgcggat
     1741 ccttgaccag ctcagcctcg acctcatccc aggccttcag ctgcgcctcg aagatcgatt
     1801 tcggagtgcg tttcaccttg acgccgtgat cgttgatcag cttctgcaga tcttctgagt
     1861 aggcgtgcca ggctttccac tcgttatccg cagccgccgc ttgcacggcg tgcttcagga
     1921 tcgcctgctg ctccttggcc aggctctcga acttcgtctt gttgaacaga atttcgaacg
     1981 tctcggcggc ctgatggtag gagcccatgt agtagagctt tgagacgtcc tgcgcaccga
     2041 agcgaaggtc ggaggtcggg ttgttatact caaacgcctc aatgacacca cggtccatag
     2101 cgggcagaat ttccggcccg gggagctgag cgacgctcag gcccattgcc tggaagagat
     2161 tgctggccag accgacggta cgatacttca gacccttgag atcctcgggg ccgctgacct
     2221 cgttcttgaa ccagcccagt ggctgcgtcg gcatcgcgaa gccgaggaag ccgacgacat
     2281 tgagcttcaa tacgtcttgg atgagctcgt tgtagagctc gagaccgcca ccgtagtaga
     2341 cccacccaag gagctggttg ccgttgaagc cataggccgg accggtgccg aacagagatg
     2401 cagcccgatg cttcccgtac cagtaggcgg gcacgctgtg cgcaccgtcg agaacgccgc
     2461 tgtggcagcc atccatgacc gagaacggct ttacgacggc gccggcgttc aaatagtcga
     2521 ctttgagacg gccttcgccc agctcgttga cgatcttcac aaaatcctgg gccatctcat
     2581 tcagcatgtc ctgggcgccc caggagccct gcatcttgag cgtgacggtt tcggcacgcg
     2641 aaacctgcgg catggcaatc gttgccgcgc ccgttacggc ggcgccggcg aggaatttac
     2701 gccgggtcgt cattgcgggt ttcttttctg acatctgcac aatcctccct gatcctaaag
     2761 cggccatgcc gcgctggtta cagctgccaa ctcacagctt ggagctgctc caaactcact
     2821 ttgcacagtc cgtcgcattc gacaagctct gacagtcaga agcggcgcta aacagctcgt
     2881 gacattcaac aaaagtataa ggatcactgc agcatggttc ggtttgccaa ctaaaggtgt
     2941 gcctgccgtt agacggcagt gatcaaaatt tcgccaaagt cacccccgga acagtggctg
     3001 ctgagagtac ggcgcagctt ggtcgtcgcc cgatggccag ccgagcgctg gcgtttgttc
     3061 aaaaggaata ggaatatgaa tatcgtcgat cgcagcctgt atctgtccgg tggccggacc
     3121 ggcattctgc tcatccacgg cctgggcggc acgccgatcg aaatgcgttt cattgcggtg
     3181 ggtttggcgc gcgccggcta cacggtgtcc tgcccacagc tcgcaggtca ctgcggcact
     3241 gcggacgatc tcagggccac gcgctggcag gactggtacg ccagcgtctg cgccgcccat
     3301 gacagacttc ggcaggagtg cgatgtcgtc atcgtcggcg gcctgtcgat gggcgcggtg
     3361 atggcactcc gacttgccgc cgaccgcccg gacagcgttc acgccgctgc cctgttcgcg
     3421 ccgaccttca ggctcgatgg ctggtcggtt ccctggtatg cgcgcctgtt ccggctggtt
     3481 tggcaccgat gggtcgcgga ccgtttcgac ttcgtcgaaa gggaaccata cggcatcaag
     3541 gatccccgcg tccgggggct cgtcaccgcc gcgcttcaga gcggcgacag ctcacaggcc
     3601 ggcgtgctca gcacgcccgg cagctccgtt ctcgagctgc gccggctcgc caacagcgtc
     3661 cgccggtctt tgcgaaacat tcggcaacct gtgctggtgc tccacccgcg cgaggacgat
     3721 cacgccagcc tgggcaatgc attcgatctg cagaaaaagc tgggtggccg tgtggaagcc
     3781 gtcgtcctgg acgattgcta tcacatcatc acgctcgacc gtcagcgcca tctcgtggtg
     3841 gaacgcacga cccgtttcgt gcgcgaggtg tgccagcagc tccaagagaa ggcggctgca
     3901 gtgaaggtcg gtcctgcaca ggtgagcccg atccgcgccg accagcctct tccggcggcc
     3961 tgaaaaatca cgggctgtga cactcggaac agcccgcgcc cgccgaccgc ctcatgatgt
     4021 cagccatcgg cagcaatttg ggcgccgcag tccacgcccg acctgatgga ggaccgtcat
     4081 gaacctgaag tacgcgaacg ggaagatcat cgcccttgca accgtcgtcc tcgcgggact
     4141 atcgctcgcg gcccccggtg ctcaggctgg gatgcgcatc catttcgggt tcccaatcgg
     4201 gagcttctac gccggcggtc atcaccacca ccatcacgcg cacagatacg agtaccgccg
     4261 actgcaggct cttcgcaggg cgagggccat cgaggcagcc cggcgcagac aagcagccgc
     4321 agaggccgca gcagcccgcg ccgaaaggaa gcgcgctgct gccctggcgg ctgcggcggc
     4381 caagcggcag gccgcggcgg agctcgcggc cgcagagcag aagaagctcg aagccgccag
     4441 agaggcggag ctcgcagcgg ttccgctgcc tgagagaaag cccgagacgc ctgcaggcga
     4501 ggccctccaa acagcggacg ccacgaccgc ggccaattca cagccggcca ccgagacgcc
     4561 tgcgaagctc gagtgcaagc gctacttcgc caacgtcggg atgaccatta gggtgccctg
     4621 ctcggaatga agcggcccgc gatcaagcaa gaagggccag gcccgccggc ctggccctat
     4681 tgcattcgaa cgtctggatt tcgatgatgg cacgcgcgat caggcgtggc cggcggcgtt
     4741 caccagatgg gtggggtcaa tcccgagctt gtagagcgcc cgctcgtatt tttcctccac
     4801 gtcgaggtcg aagacgagct ccaagtccgc ctggcagctc agccagccgt tggcgtgaat
     4861 ttcactctcg agctgcccgg gcgcccagcc ggcatacccg agagcgagga tcgcccgatc
     4921 cggtccctca ccggcggcca gcgccttcaa gatatcgatg gttgcggtga ggcagacgtt
     4981 gtcgtcgatc ggcagtgtcg aggagggcgt gaaataatcc gagctgtgca gaacgaagcc
     5041 gcgctcaggc tcgacgggac cgcccagatg aacgggcatg tgcagcattt tggaaggcac
     5101 ggatatttcg gcgtcttcgt cgagaagctg caatctctcc aggagatcgg ggaaactgag
     5161 gttcggcgcg cgttggttga cgatgagccc catagcaccc tcgtcggaat gggcgcacat
     5221 gtagatcacc gagcgtgcga accgcttgtc ggccatggca ggcatcgcaa tgagcaactg
     5281 cccttcgaga tagctgctgc cagccaagtg ttgtgaccgc cgtttcactg tcatcctagg
     5341 actctagcct caggcgtctc caccgtgaag ctggaaatgc gcccgaaacc atattctgct
     5401 ccctcaggcc caaatcattg tgacttgccg caaccgcaac ctcgggtaca taggcagcgg
     5461 tgtcggaccg gcaagaggag gctttggtcg tgcatggttg cagggtgcac cttctggcgt
     5521 cggcgttggc catcctgctc gcgttctcga cgcctgcagc ggcagacccg ctggcttccc
     5581 cttgggtcga ggcgcacggc ggcaaggcgc gcctgatcgc gggaactgtc gactcggccg
     5641 gacgtagcgc accaaagctc ctggccggaa tcgaaatgcg gctcgcgccg ggttggaaga
     5701 cctattggcg ctctccaggc gactcaggtg gccttccgcc gcagttcgat tggtcgcagt
     5761 cggcgaacct cgatgtcgcg aaggtgctct acccggcacc gagccggttc aaggacgccc
     5821 ttggggatgc gctcggctat tcgggatcgg tcatctttcc ggtcgagctc cggcccaaga
     5881 attcttccaa gcccatcgag ttgcgcctct cgctgcttta cgggatttgc cacgaaatct
     5941 gcatccccgc tgaagccaag ttgacgcttc agatccccgc gacggcgacc attcccgccg
     6001 acccgggcat cagcgccgcc ctctcggcgg tgcctcgcag ctccgacgag cgccggccga
     6061 acgatcccga gttgatttcg aaggcggccc gtctcgatgg cgatcggccg aggctgatcc
     6121 tcgaagcccg cttccccaaa ggcgcgtcat ccggtgacat gttcatcgaa gtcctggagg
     6181 gcggttttgt gccgctgcca gtgaagacac aaacccgcgg cgacacggcc cgcttcgagg
     6241 cagatttgac acagggcgcc gagcccgacg agctgcgcgg caagactctg cgtatcacaa
     6301 tggtctccga cgcaggtcag tccgaagcct tgtggaagct cgagtgagct cacggggccc
     6361 cctgaaaaac cgctctttga tctgagcgga acaacggcgt atagctcgga tcactacctg
     6421 cagcaggtag tggtacccag actgaaaaat tccggagagc atcatgacaa tcaacgtggg
     6481 tgatcggctg cctgaagcga ctttcacggt gatgggcccc gagggcccga agcaggtgac
     6541 gaccggcgag gttttcggcg gcaagaaagt ggcgctgttc gccgtccccg gcgcctatac
     6601 cccgacctgc cacaagcagc acatgccggg cttcgtcgcc aatgcgccag agttccagaa
     6661 caagggcatc gacatgatcg cctgcacggc tgtgaacgac atcttcgtgc tgacgcaatg
     6721 ggccaaggac tccggcgccg aaggcaagat caccatgctg gccgacggca gcgccgagtt
     6781 cgcgcggaaa atcggcctcg acatcgacct ctctgcccgc ggcctcggcg tccgctccaa
     6841 gcgctactcc atgatcgtgg acgatggcgt ggtgaaggtc ctcaatgtcg aggatgctcc
     6901 gcctcagcac gacaagtcga gcgccgtgaa ccttctggcc tgctcattct gagttgttgc
     6961 tgattaactc gtgtcagttg gctgcatccc gggcacgagg cccgggattg ccggcttcca
     7021 aagacggcga gcttatccca ccgacagata ggtcgtcata acatccgggt tggcacgcgt
     7081 ctccgccgcg gtgccgcgat ggacgatctc accctgctcg atgatcgtgg cttcatcgcc
     7141 gatctcgagt gcgatttcgg cattctgctc cacgagcagg atgccgaggt tctcctcctt
     7201 gagccgcagc agcgtgtcca cgacgatatc gacgataacg ggtgccaagc cctgggaggg
     7261 ctcgtcgagc agcaggaact tggggcccga catgagggcg cgcccgatgg cgaccatctg
     7321 ttgctcgccg ccggagaggt gacctcccat gcgcccgagc aacggcttga gcggaggaaa
     7381 gagatccagg acacgctccg ccggccacgg cgatccgggg tactcgctga tggcgagatt
     7441 ctcgcgcacg gtgaggcctg agaagacgcc gcgatactcc ggaacatagg aaagcccggc
     7501 gcgggtgatg agatgcggcg gccgccctcc gatgctctgg ccgtcgagcg ttatggaccc
     7561 cgaccacttg tccatcaggc cgaagatggt tctgagcgtg gtcgtcttgc ccgcaccatt
     7621 gcggccgagc aggacgtgga tggtcccctg ctccacgacc atcgagatat tctggagaac
     7681 gtggaagttg ccgtaatagg cgtggacacc ggcgagcgac aggctcatgc acgcctcccc
     7741 agataagctt cgcggacctt cgggttggcg cggatctcct gaggcgttcc ttcggccagc
     7801 acctcgccga agtacatgac ggtgatccgg tctgagattc tcatgaccac gtccatcttg
     7861 tgctcgatca gcacgatggt gtagcggccc ttgaggctct cgatcaggtg catcacgcgt
     7921 tgtgtatcgg atcgggagag cccggccgtc ggctcatcga gcagcagcag cttcggcttc
     7981 ggagccagcg cgatggcaaa gtcgagcagc cgctggtcgc cgtgcgccaa ctcggtagca
     8041 ggggaattgg cgtaacgatc gaggccgacc tcctcgaggg tctggtagat ggtttccgtc
     8101 acctccggat gcgcatcggc aggccgcaga acctccacgc gcgtgccggc gaacgcgcga
     8161 aatgcagaca cccagacatt ctgagcgacc gtcaaattcg agaaaatgct tgtccgctga
     8221 aaggaccgcg cgacgccgaa cgcgggcagc tcatgggttt tccgcccgga aatgacgtga
     8281 ccttcgaacc gcacctctcc ggatgtcggc ttcagctcac tggtgatgca gttgaagagt
     8341 gtcgtcttac ctgcaccatt ggggccgatc acggtatgca atgcgccagg cggcacttca
     8401 aagctgacgt cattgacggc cttcactgcg ccgaagtgct tcgagaccga gatgcactcg
     8461 agacccgtgg tcatgagccg gcctccatct ccttcggctt ggcggccgcg cccttctggg
     8521 taagcttgcc cgtgaggaac tcggccatgc ccgcgactcc ggttctgaag aacaggacga
     8581 ccagggcgaa aacgatgccg aggatcatgg gccagcgatc ccaatagtgc gatacggtct
     8641 cctggagcca ggtgtagaac gcggcgccga taaccgggcc tatgaaactc ccgctgcctc
     8701 cgagcaacgt catgaaaacg atgtcgccgg aaaagcgcca gtcgactttg tcgatcggaa
     8761 caatgccgaa gacgaacgca aagatcgccc cggcaaggcc ggtgaacatg ccggccatca
     8821 cgaatgcgat gagcttgtag cgtccgacgt catggccggt tgccgccgca cgtcctggat
     8881 tgtcgcggat cgcctgcaga atgcgccccg tcgggctgtc gacgaagagc ttcatcacgc
     8941 acagcgcaca gataaagacg agcacagtga aaacataaaa cgaccattga tcgtcgatga
     9001 gcggcggccg ccgcacaccg ggcagcccgt cctcaccgcc cgtcatggtc gtccaggaat
     9061 acgcgatgaa gaacaccatc tgattgaatg cgaacgtgat catgatgaag tagaggccgg
     9121 atcggcgcac acaaacgagg ccgatcagga atgcgatgac ggccgaaaaa agcatcgcga
     9181 ccgggatcgc caaaaaagcg gaaatcccat acctcgtggc gaggataccg gcgcaataag
     9241 cgccaacacc aaagaacaga gcctgtccga acgacagcat ccccgtatag cccagcagca
     9301 ggttggtcgc gacggccgca atgccgagcg tcagcacttc cgccgcgacg accggccgtt
     9361 gaaggaacgg gagccctacg atcagtaaag caatgacgat caggctcgga agactgaaca
     9421 gtttctgcat ttagcgtgtt ccgaaaagac cctgcggcct caggatgatg gtcaggccca
     9481 tgagcacgta gatcgcgatg ttggaggctt cgggccacca gatggtggtc aagctctgcg
     9541 caactccgat gagtatgcct gacgcgatgg cacctagaat gctgcccagg ccggcgatga
     9601 ccacgatcac gaagctgatc ggcagcatgt cggcgcccat gctcggcgtc agccccatga
     9661 tcggcgtggc cagcgcgccg gccagccccg ccagataagc gccgagcgcg aatgtccaca
     9721 tgaagacacg gttgatgtca gtgccaagca cagcaaccat ctcgcggttc tccatgccgg
     9781 ctctcacgat cgcaccgacg ttcgttcgcg tgaggaacag ccagagcgcg acggtgatca
     9841 gaacggtcat tccgatcagg gcgaggcgat aggccgggta aacggtaaat ccaaggttca
     9901 cgacgccgga cagcgccttc ggcgtcagca cggaaaagcc gactggcccc cagatgagga
     9961 tgatcgcttc ctgcaggatc agcgccatcg cgaagaggaa aaggagctgg tagtcgtgat
    10021 gtgctgcgta aaggcgtcgg accccctggc tctcgatgag gaatgcgacg aggatcacga
    10081 gcagtggccc gatgagcacc gcaaggtaga aggagccggt caggtttacg accgtatagg
    10141 tgaaataggc gccgaacgcg tacaaaccgc cgtgcgcgaa attcgggata ttgagcatcc
    10201 cgagcacgag tgtcaggccg acaccaacga gcgcgtagag cattccggtc gcaagaccgt
    10261 tcagcaattg gctggcgatc agaaaggtca tgaaagtccc tgcaaggcgc gggcgatcag
    10321 ggtcggatca gaacaaatgc tgcattggcc ctcgcaagcg gaccaatgca gcatatcgcg
    10381 ctcagaaccc accgatgtcc ttgcactcgt tcagctcggg cggctgctcg atcgaactct
    10441 cgtctatgac cgtggccagg tcgtactcgt ccttcatctc ggacttcgac ttcgtcttga
    10501 gaatgaagta gggccggttg atctggtgcg ttttgggatt gaccgacgtt tcaccgagga
    10561 tggtcttgcc cttgaagttc tcaagcgcct cgatcgcctt ttgtggctct gcactgttgg
    10621 cgagctgaat cccggtaagg atggcatgcg tcatgccgta agccgctgct ggcgcgtatc
    10681 cgggaggaac gccgccgctc ttcttcctgt aggcctcgac gaaggccttg ttttcgggat
    10741 tgtcgatcgt ccagtagtaa ttggtgccga cctggagatt ttcgcgcatt tccggatcga
    10801 gctggatcaa ctcctcggtt cccgaggacc aagccatgat caccggcttc ttcggtgtaa
    10861 agccgaagtt atagatctgg cgcagcgcgg tcacggcatc gataccgaag ttgaccatca
    10921 tgatcgcatc cggattggcc gcaacggcct tcgtgatgta cgaggagaac tcgcggtgcc
    10981 ccaacggatg gcgatcgacg ccggcaatcg tcagtccaaa cttcggcgcc agcatttctg
    11041 catatttctg cagagaccag ccgaacgcat agtcggcaat aaaaaggtaa agtgattttg
    11101 cctcggggtg gttctttttg aagacccgca ggtttgcctc gaccgcggtg tacgccgggg
    11161 ctgcccactg gaacgtgtaa cgatggcagc gcttgccgga aaggtcctct gtcccgccac
    11221 tgaacgcgta catgaccttg ttcttctgcg cgacgtccga gacggcgagg ccgacacccg
    11281 acgaccacgg gccgaccatg aatttcacgc cctccccgtc gatcgcctcc tgtgcccgcc
    11341 tcgttgccgt gcccgctttc gtctcgctgt cgcgggtgat gtacttgatc ttcttgccgg
    11401 cagcctgata gccaaactgc tcgagggcga cttgcgctcc gtgatcgagc agcgggccaa
    11461 catccgcata aggtccgctg aacggccaga gtccgagcaa cacgatctcg tcatccgccg
    11521 catttgccgt catggccgtg gcggccagcg caatcgtcgc tgcagccgcc atgcccttca
    11581 gaccgagtga aactcgtttc ataggcgttc tcccttggtt gtctgaggtt attcttagtg
    11641 cgggggccgt tcgcggcttg agcgacccgg ccctaccctc cagcatcagc cgtgggtctg
    11701 attgaagccc tccacgttat tcaagtagtt cacacaggtg tcgaccgatt cgacatcggc
    11761 aaactttgca tcgatatcga acaggttcca ggcaacagcg ccgggcacac ggtcgccgat
    11821 gcattcacga accgcaatcg tccggaaacc ctcggccagg ccatcgcaga tcgtggtgcg
    11881 cacgcaggca gttgccgtca cgccggtcac gagaatggtg tcgacgcccg ccgagcggag
    11941 atagccggca agcggcgtgc cgctgaacgc gctggcgcgc tttttcagca tcacgtattc
    12001 gccttcgatc ggcgcaatgc gggagtcgat ggcccacagg tgctcgtctg cgatgtcgac
    12061 cgcctccacg gggattttgt aatgccacaa gcccatatcg gacgtgcggt cgttccggtt
    12121 cgtgttctgg taggcggtcg tcacgtgaac cacgacgtgc ccattcttgc ggcaggcctc
    12181 gagaagccgc tgcatgccgg ggatgatttc ttcgtcaatc ttttcgcagg tgaaagggtt
    12241 gccgggccgg gtccaggcat tcgcgagatc gacgctgaca agggcaggct tgcgcccgaa
    12301 ccctatccgg cgctgaaagc cgcgttgctg atagatcttc gtgctttcgt cgaatgccct
    12361 ctggagcatc tcgtccagct tgccgagatc catcgtttcc tcccttggcg cggcccccaa
    12421 tcgagctgcc gcgtcctact tttgtcttag gctacatcaa agcctgggaa ttcgaaaaag
    12481 ggaagagcga tgatgtcggg caatgttcac ccgcgagaaa agctcttcat ttggtaacag
    12541 cggggcagct gacacggggg caatccgtgc cctcagctcc cgcggatctc gcggatcgcc
    12601 tttcgcgcaa gctcgtccgc acgctcgttt tccgggtggc cagcgtgtcc cttgacccag
    12661 tgccagcgga tttcgtgggg cttcagggct tcatcaaggc gctgccagag ctcggcattc
    12721 ttcaccggtt tcttgtcggc cgtgcgccag ttgttgcgct tccagctatg gatccaggcg
    12781 gtgatgccgt tccggagata ggcgctgtcg gtgtagaggt cgacgatcga gggacgcttc
    12841 aaggcctcga gcgccctgat ggctgccatc agctccatgc ggttgttggt cgtgacggga
    12901 tcgccacccc acagctcctt ccggtgtggg cccgagatca ggatggcgcc ccagccgccc
    12961 ggacccggat ttccggagca ggctccatcc gtgtagatct cgacgacggg acggcggctc
    13021 gtcattgggc ggtttcgagc ccgtactcgc cgaccgagcg caccgtgcga tgaaagcgca
    13081 gccggcgccg gtattcgagg ggattgtgcg gtttgacgag ggcgttttgt gatgtgtgca
    13141 gccagtcgta cgcgcgcgtc agcagaaacc tgagcgctgc gccgcgggcc agaatgggca
    13201 aggcttcgtg ctctgccggc tcgagctcac gcaccgcggt gtaacccttc agcaatgcac
    13261 ggctcttggt gatgttgaat gcgaagtcag cctcgaaaca ccaggcattg aggcacaccg
    13321 caatgtcgta ggcgaagaaa tcgttgcagg cgaaatagaa gtcgatcagg cccgacagct
    13381 cggtgccacg gaaaaacaca ttatcgggaa agaggtcggc atggatgacg ccgcacggca
    13441 ggccctgcgg ccaagccggc agcagttctt cgagctcgcg gcgtatttca tcaccgagcc
    13501 ccggcgccac ctcgtcggcg cgatcctcga accgctcgaa cagcgcttcc cagcccttgg
    13561 gtcccagtgc gttttcacgc gtgagcttgt aatcccggcc ggccagatgc agcttcgcca
    13621 gggcctcacc gacggcatga cagtgctccg gccgcgggcg ccggatccac atgccatcga
    13681 ggaacgtcac cagcgccgcc ggccggccag cgagctcgtt gagcatcacg ccgtcgcgat
    13741 cccgaaccgg cgtgggacac gtgatgcccg ctttcgctag gtgctccatg agccccaaga
    13801 agaacggcag gtcttcacgc tgcacccgct tctcataaag cgtcaggatg aagcggccct
    13861 tggcggtctg cacgagatag ttggtgttct caaccccctc cgcgattccc ttgcaggaga
    13921 gcaattcgcc gagcccgtag cgttcgataa accgggccag ctcgtcgtcc gcgacctcgg
    13981 tatagaccgc catggcgtca ggcactcgcg gacagcttgc gggcgggggc aaaatcgcgc
    14041 aattcgcgcg gcacgttgaa ggcgatgtgc tcctgcgccg tgctgacggt ctcgaccgtc
    14101 acatcaaagc gctcgcggaa cgcatcgacg atctcgttca ccagctcctc gggtgcagaa
    14161 gccccggcgg tcaggccgac ggtgcgcaca cccttaaatg cggcccagtc cacgtctgct
    14221 gcccgctgca cgagcatggc acgctggcaa ccggcccttt cggcgacttc gaccagccgc
    14281 agtgagttcg agctgttcgg cgccccgacg acgaggaacc gctctactct cggggcaatg
    14341 gacttcaccg ccgcctggcg attcgtcgtc gcatagcaaa tatcttcttt gtggggtcca
    14401 acgatgtgcg gaaaccgctc cttcagcacc gcaacgatct ctgccgtatc atcgacggac
    14461 aatgtcgtct gtgtggcgta agcgagcttc tgcgggtcgc gcggcgtgaa tgcgcgggcc
    14521 tgctcgaccg tctcgatcag cgtgaccgct ccctcgggga gctggcccat ggtgccgatg
    14581 atctcagggt gccctgcatg gccgatcagc acgagctcgc gaccgtgcgc ataatgctga
    14641 tgcgcctcct tgtgcacctt cgacaccaac gggcatgtcg catccaggtg gaacaggttg
    14701 cgcgccttgg ctgcggccgg gacccacttc gggacgccgt gtgccgagaa gatcacaggc
    14761 gcatcggttt ccggaacctc gtccaactcc tccacaaaaa tggcgccttt cgctcgaaga
    14821 gaatcgacca cgaatcggtt gtgaacgatc tcgtgccgca cgtagacggg aggtccgtac
    14881 ttggccagcg ccagctcgac gatctggatc gcgcgatcca cgccagcgca aaacccgcgc
    14941 ggcgccgcca atatcaacgt cattttttcc cgcaggccac gctggcactg gtcaacggct
    15001 tcagaggcag acataccttt gtcttggtcc ttcgagtcga gtcaccctcg ttgctacgcg
    15061 cttggccgct ccgagtcgag ggtgttgggc ggcctgcgcc acagtgcggc aagcgctcct
    15121 atggcattgg ggccctacgc cgaggtgcta gacagcaaac aaccggcact ttccagccgg
    15181 gaccggaata cgaaacgagc gggacaaccg tgatcaaact caggactgcc gcggccccca
    15241 gagcccttca gattctcttt tcaggattcg cactcacctt gctggcaggc tgcgggatgt
    15301 cgagcctgac gtccggtatt ggcggcggca tgctcggcgg ctcgtccagc tcgagcacgg
    15361 aagtcgcctc cgtgagcgag gagcagctcc tgtcggccgc caaggcggat tacgggacca
    15421 caggctcgtc cggcatcgac ttggcgcacg gctgcccgcg gttcgtcatc tggccgcgcg
    15481 acaacagcgt cacgatttac gagcagggcc gggtcgggga cgcgctggcg gtcatgcatc
    15541 gaggtgagat caccaaaact gcgcgcgagt gccatatcga gaacggccgc gtgactgtga
    15601 agtatggctt ctcaggccgc gttctgctcg gcccccgcgg gaagccgggc agcgtcaagt
    15661 tgcctgtcaa tgtcttcgtg acggacgcaa ggcgcgagaa gatcgcccaa gatacgatgg
    15721 ccgtcgatgt cgacgtcgcc ctcgaacggc cgatcgggta tttctcggtc gtgcgtaccg
    15781 tctcctttac gatacccgaa ggtacccgcc ccgccgagta cgaggtcttc gtcggcttcg
    15841 atcgtaatct gccgaacgct ggctgatacc gctcgccttt gttgtgcgga acggcctgcc
    15901 cgcaggccac cgcacccgca atccagattg acttcgcctt tgccggccct atgctgaggc
    15961 attgccagtc gacacttgcg ggagagttcg gagcacaggc agtgcccggc gccgaaggag
    16021 caaccgcccc ggaacctctc aggcaaaagg accgcaagtt gatggcgctc tggaaagcgg
    16081 ccgtggcaat ccgtcgcccg gtccaccgaa ggggttaaga tccggcttcc gcaccgttgc
    16141 gcggctggcg gggttgactc tctcaggttc cgagacagag ggggctcgaa gcgggtgtct
    16201 cggcactcgc tcagctgggc cgtcagttct cgggaacttt gcatgagcgc cgatgccgcc
    16261 aatgaagccc tgaagcgaac accgctttac gacctccacg tcgagctcgg cgcgaagatg
    16321 gtgcctttcg ccggctatga gatgccggtt cagtaccagc tggggattct gaaagagcac
    16381 cagcatacgc gcgcgcgtgc cggcctgttc gacgtgtcac acatgggtca ggccaagctc
    16441 gtcggccccg atcaccagac gacggctgcg gccctcgagg cgctcgttcc ggccgacatc
    16501 ctcaacctga aaccagggat gcagcgctac acgcagctgc tgaatcccca aggcggcatc
    16561 gtcgacgacc tgatggtcgc acgcccggcc gaccctgccg aggacggcac tttgttcctc
    16621 gtcgtgaacg cctcccgcaa ggaggtcgac tacgagtaca tctcggccca tcttccggac
    16681 acggtgcggc tcgagccgca gccggaccgt gccctccttg ccctccaggg gccggaggcc
    16741 gcggcaacgc tctccaagca ttctcccgaa gccgtgactc tcggcttcat gggcgtgcgt
    16801 cgcgtccctg tcgggccgtt cgagtgtcac gtcagccggt ccggctatac cggcgaggac
    16861 gggttcgaaa tttctgtagc agccgatcag gccgtcgacc ttgcgcgcct cctgctggct
    16921 gaagaggcgg tgcagccaat cggtcttggc gcccgcgatt cactgcggct ggaagctggg
    16981 ctctgcctct atggccatga gctcgacgag atgatctcgc cggtcgaggc gggtctcgcc
    17041 tggtccattc agaagcgccg gcgcgagggt ggcggcttcc ccggcgccga gcgaatccag
    17101 aaggagctgc gcgaaggtcc tgcacggttg cgcgtgggca tcaagcccga gggccgcgcc
    17161 cccgcccgtg acggcacgga gatcctggag ccttccggtg cccggatcgg catcatcacg
    17221 tccggcggtt tcgggccgac ggtaaacgga ccgatcgcca tgggctatgt cgagtcctca
    17281 tttgccaagc cgggcacggc cgtccagctg atggtccgtg gcaagccgca gccggcacac
    17341 atcgtcgaac tgcccttcgt acctcatcgc tatcttcgca aggcctgaaa cgggagaacc
    17401 catccatggc gctcgaacgc ttcaccgagg accacgagta tatccgcgtc gatggcgaca
    17461 tcggcacggt cggcatcact gtgcacgccc aggagcagct cggcgacgtg gtcttcgtcg
    17521 aggttccgga ggtcggtcgc acggtgaagc aaaaggagtc gacggccgtc gtcgagtccg
    17581 tgaaggccgc gagcgagatc tatgcgccgg tctccggcga agtcgtcgag gtgaataccg
    17641 agctggcggc gaacccggcc ctggtgaacg aggatgccga gggcaaagcc tggttcttca
    17701 agctgcgcat cgccgacagc tcggaactca gcagcctgat ggatcgggca gcctacgagg
    17761 aatttctgaa acagagctga tcggttcgcc ttccctgcat gcgggggcgg tcaaatgggg
    17821 gcggccagat ctttgtagcg ctggcttcta ccctggggcc ctgccctcta ccgatcgctg
    17881 tagcggggag cgaatggacg tgcgactcat gatggagcgg tgctgatgcg ttatcttccc
    17941 ctcgagcagg ccgatcgcga ggcgatgctc gcccggatcg gcgtgccggc cgtggacgcg
    18001 ctgtttgcca gcgtgccagc cgacaagctc atgaaggagt tgcctgatct tcccagaacg
    18061 aagggcgagc tggaggtcga gcgcgagctg tcgaagatgg ccgcccgcaa cgtgccggcc
    18121 ggctccgtgc cgttcttcgt cggtacgggc gcgtaccgcc atcatgttcc ggcgacagtg
    18181 gatcacctga tccagcgttc cgagttcctc acctcctaca cgccctatca gcccgagatc
    18241 agccagggca cgctgcagac gctgttcgag ttccagacgc aggtcgcact gctcaccggc
    18301 atggaggtcg ccaacgcgtc catgtacgac ggctcgacgg cgtgcggaga ggcggtgctg
    18361 atggcgaacc gcgtgacccg gcggaagaag gcggtgctgt cgggcgggct gcatccccat
    18421 taccgctccg tcgcagaaac gctgtcacgt tggggcggct cggatgtcgt cgcgctcgat
    18481 cccgatccca ccggcgcaga ggacatcatc tcccagatcg acgaccagac gtcctgcgtc
    18541 gtggtgcagt atccgaacgc ctacggcacg atccgcgacc tcgctcccat cgccgaagcg
    18601 gctcatgccc aaggtgcgct gctgatcgcg gtcgtgacag aaattgtatc cctgggcctg
    18661 ttgaagccgc cgggcgagat gggcgctgat atcgtcgcgg ccgaaggcca gtccatcggc
    18721 aacggcctga acttcggcgg accctatgtc ggcctgttcg ccacgcgcga gaagttcgtc
    18781 cgccagatgc cgggccggct cgcgggcgag accgtcgatg ccgaaggccg gcgcggcttc
    18841 gtgctgacgc tgtcgacgcg cgagcagcac atccggcgcg aaaaggcgac gtcgaatatc
    18901 tgcaccaact ccggcctgtg cgcgctggcc ttcacgatcc acatgacgct gcttggcgag
    18961 gcggggctgc ggcggctcgc tcagatcaat cacgcgaatg ccgtgacgct ggccgatcag
    19021 ctctcccgca tcggaggtat cgaggtgatc acgccggcct tcttcaacga gttcaccatc
    19081 cgtgttccgg gtgatgcgca gcagatcatc gatgcgctcg caaagaaggg cgtgatgggc
    19141 ggcgtgcccg tctcacgact ggagccgagc cgaaatgacc tccgcgacct catcatcgtc
    19201 gcatcgaccg agatcaacac cgacgaggac cgcgcggcct atgtcaaggc gctgcaggag
    19261 gtgctctgat ggctatgaac tcacaggggc ggccgacaac acccggtgcg gcgggtccgg
    19321 cggcgagcgg cattgagaca ttcacggggc atcgcgggct cgaccacgag gaacttctga
    19381 tcttcgagtc gggcagcctg tcgaagagcg gtgtggacct gcccgagccg aagccggtca
    19441 agtcacggct caacggcctc gagcgaaatg gaccgatcgg cctgccgggg ctcaccgagc
    19501 ctgaggccat gcgccactac gtgcggcttt cgcagcgcaa ttacgccatc gataccgggc
    19561 tttatccgct cggctcctgc acgatgaagc acaacccgcg cctcaacgaa aaaatggcgc
    19621 ggctgccagg cttcggcgac gtccatccac tgcagccgca gcagacggtg cagggcgcaa
    19681 tcgagctcat ggaggcgctc tcgggctatc tgctcgagct gaccggcatg aaggccgtcg
    19741 cgctctcacc caaggcgggt gcccacggcg agctttgcgg catgctggcc atcaaggcag
    19801 cgcacgaggc cgccgggtcg aagcgcagcg tggtgctcgt cccggagtcg gcccacggaa
    19861 ccaacccggc gacggccgcc ttcatgggct acacggtgcg ctcgatccca gcccgcgagg
    19921 acggcacggt gtcggccgat gcggtgcgcg aagcgctcgg acccgacgtt gcggcgatca
    19981 tgttgacgaa ccccaacacc tgcggcctgt tcgagccgga ggtggtcgag atcgccaagg
    20041 ccgtgcacga tgccggcgcc tatttctact gcgacggagc caacttcaac gccatcgtcg
    20101 gcaaggttcg cccgggtgag ctcggcgtcg acgccatgca catcaacctg cacaagacct
    20161 tctcgacgcc gcacggcggc ggtggcccgg ggtccgggcc ggtcgtactc tcggatgcgt
    20221 tggcgaagtt tgccccggtt ccgttcgtgg tccgggatgg cgacgagctc cgcctcatcg
    20281 aggacatcag cgaggctgaa gcccgcggac agaagccgtt cgggcgccta accgccttcc
    20341 acggccagat gggcatgttc gtccgtgccc tgacttacat gatgtcccac ggcgccgacg
    20401 gcatgcggca ggcctccgag gatgcggtcc tcgccgcgaa ctacgtgcgc gtgggcctca
    20461 aggatctctt cagccagccc ttcggcgacc agccctgcat gcacgaggtg ctgttcgacg
    20521 acaagttcct gaaggacacc ggcgtcacga cgcttgattt cgcaaaggcg atgatcgacg
    20581 aaggctacca cccgatgacg gtgtacttcc cgcttgtcgt acatggcgcg atgctgatcg
    20641 agccgacgga gtcggagtcg aaggacaacc tcgacctgtt catcgccacg atgcgcgatc
    20701 ttgccgaggc cgcgaagcgt ggcgatgtgg accgcttcaa gcaggccccg cgcctcgctc
    20761 cacgccgccg cctggacgag acaagggctg cccgcgagcc gaagcttcgc tgggctccgg
    20821 acgcggcgcg caagcaagct gccgagtagg tggcttgacc gggatggggc tgagttagct
    20881 gagccccatc tgctgtgctt cccaaatact tgcgatcatg aattgccgcc gctggcttgg
    20941 cctctgcccg ctcgcgaccg agtttcgagt tcggcttgca tgcttgtgcg acatcgaggg
    21001 gaaccgtgcg gttcgacctc cagttgaggt gcagatgcac tgaaattctg gagatcgaca
    21061 tgaccgacgt acgtgaagtt ttcgtttcgg gcctgcgcga cgcctatgcg atggagaaac
    21121 aggccaagga catgatggag agccaagcca gccggctgga ggactatccg gagatgcagt
    21181 cgcgggcggc ccagcatgcg cgcgagaccg atgctcagat cgaccggctg cgccaatgcc
    21241 tcgagatgat tggcgagtcc ccttccatgt tcaaggaatt cggcacacga gcgatggcca
    21301 atctgcagtc catgctgagc gcggcctcga ccgacgagcc gataaaggag acgcttaccg
    21361 gcttcgcctt cgagaacttc gagatcgcct cttaccgcag cctgatcgcc ttcgccaagc
    21421 agctcggcga gcagcgcatg gttccacttc tcgaggagag cctgcgcgag gaggagcgga
    21481 tggcgaactg ggtgggcgat cacatcgagt ccatcaccga acaattcgcc tcgagggcaa
    21541 gcgccaccgc ctgacgagaa gtgcgccgcg ctccttgtcg ctcggggcgc ggcgcatgct
    21601 ttcgtggtcg cccctacgtc ttcgggggtg cgccgcgcac ggcacggcct gccgaatgag
    21661 ccggcggatc gctggctggg aacgactcga tcgatccttc ctcgacgtac aggtcgtcag
    21721 ctacagcatc ctgctcctca gaaatccgag caggcgcttt ccctgctaca ggcagctcct
    21781 ccttgccggt gagcagctgg cgcagcacga actgcaggat cccgccgtgc cgatagtaga
    21841 gcacctcctg cggagtatcg atcctcgcga tcgcctcgaa ggtctgctcg ctcccatcct
    21901 cgcgctgcac acggacggtc agattgcggg tattcgcaaa tccattcgcg acaccctcgg
    21961 tgatgccctc gatcgcgaac gtctccttgc ccgtcaaccc tagactctcg accgactggc
    22021 cgggaatgaa ctgcagcggc aggataccca tgccgacgag gttggagcgg tggatgcgct
    22081 cgaagctctc ggcaatgacc gccttcacgc cctggagatg cgggcccttg gccgcccagt
    22141 cgcgactcga gccggagcca tactccttgc cggccaggat gatgagcggc gtaccctgcg
    22201 cgatgtagcg ctgggaggca tcgtagatcg tc
//
LOCUS       DASVDU010000064        20181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_346099, whole genome shotgun sequence.
ACCESSION   DASVDU010000064 DASVDU010000000
VERSION     DASVDU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20181
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346099"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF226_04370"
     CDS             <1..223
                     /locus_tag="VF226_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7073253.1"
                     /translation="GLAIASVTREEGLDFVPLHWERYDLVVRRVEYFEPPLQRLFAFT
                     RTELFSEQAKALGGYDIAQTGTVVFNPRR"
     gene            complement(255..407)
                     /locus_tag="VF226_04375"
     CDS             complement(255..407)
                     /locus_tag="VF226_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073254.1"
                     /translation="MYAVCSILNVPIGEYTRESIPLFAAVSLVTLVLIFVPGIVLIVP
                     NMIFGR"
     gene            complement(551..1012)
                     /locus_tag="VF226_04380"
     CDS             complement(551..1012)
                     /locus_tag="VF226_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7073255.1"
                     /translation="MKSRLDYWKVSPQGFAALRHLNDYVEGCGLERSLLELVKTRASQ
                     INGCAYCLDMHTKDARAAGESEQRLYTLSAWRETPFFSERERAALAWTEAITKISEHP
                     IEDALYTELQQYFSEKEIVDLTLAIITINGWNRLAIPFRTPAGSYKPRASG"
     gene            complement(1306..2199)
                     /locus_tag="VF226_04385"
     CDS             complement(1306..2199)
                     /locus_tag="VF226_04385"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX7073256.1"
                     /translation="MASGRASLVSLSLIGDMPWIRPGAGGLRQSATPKPGEAWTWLEA
                     ELARAKSHIAEQGLKIVLTPADLDRALTGEPHVVLSVEGASFLDDGIAGLKPAHDAGI
                     RHIQLVHYVRNSIGDFQTEAPRHGGLTAFGRRVIEECNRLGILVDLAHSTATAVRQAL
                     ETSRTPVVWSHSSVARNDVPRRKLGPLQARRLGIDEARAIASKGGVIGLWALRSDVGA
                     TVEAYADRLIEMAAWLGDDHVAFGTDMNALSGSPISTYADLRRAVRYMERRGLDRIRL
                     QKIAIGNYARVLRHAMEAAGH"
     gene            complement(2443..3702)
                     /locus_tag="VF226_04390"
     CDS             complement(2443..3702)
                     /locus_tag="VF226_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073257.1"
                     /translation="MRSLTFAAIAAVAWAAGHLPLSAAEFTYNEKVNQEIAKKLSIPV
                     YFAVPDSARAPLPPSFNTSDRLVEFKHPEANGNSAGIGLRLVIAKRSGFAKRMAKSGL
                     LQTGDILLSFRPEWGGAGTYPNLQMGVSHAGLAYVKDGAVYQLDNPMDAEHLGPRLKG
                     DFSGDHYRKLKFIHVIRPRGLTDAERANILAWATRLGGGAKRIYPKQISFNTDYNAPK
                     YSPGKPLDFVKNVARSALGHDDVVPTDMFCSEFVWSILALRKCDPKTTAESFNGTGVP
                     SCVDPPMTPMKATGNYISRRSASSHAGLADGPLLVIDAMKLPDAERNALLHNVFVENP
                     SKLANMSSGHREVAQAMQPKFTPLERYYKAAAEGGFHRIQAFFLGNAFRRAIPDNYSP
                     TSYLINTLLPPNNRTRTMDYVATIMFE"
     gene            3903..4574
                     /locus_tag="VF226_04395"
     CDS             3903..4574
                     /locus_tag="VF226_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073258.1"
                     /translation="MAFGCFSVWIAWLGRESGPPSRCYGGVVGGRLEGGRRLPLWGEN
                     YSAYSTLGYLAGRTFMHGTVRDAILAAYAELNETHPDLRYVYAESGWPWGGPFSPHRS
                     HMNGTSVDFHVPVRTLDGAVSELPASVFNKLGYAIEFDRSGQWNGYKIDFEAMALHLI
                     ALDRAARASGISLRRVIFDVQLQPKLFATEAGRPLPKVMRFNQRQSWVRHDEHYHVDF
                     NVPCR"
     gene            5013..6032
                     /locus_tag="VF226_04400"
     CDS             5013..6032
                     /locus_tag="VF226_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX7073259.1"
                     /translation="MTENVTITLDELWRLGIEVLEANGFSKDHARTINASIWACQRDE
                     CHSHGAYRLISCVETVRAGKISPVAEPEVSHPAPGIVRIDAKGAYSLLSFEVGRPLLV
                     EKARKQGVAVLAINHCYHFSALWPEVEALAKDGVAAIAMTPGTSTVAPAGGTKPVFGT
                     NPFAFAWPRPNGNPYVFDFATSVVARGEIELHKRAGKPIPTGWAIDANGEPTTDPARA
                     LEGALLPFGSYKGTAIATMIELLAGPLIGDLLSPEAKEYDAGANVTPYRGELVIAFDP
                     VSFLGAEADQHLQRAERIFEEITSQGARLPSQRRYEARARSLASGTVTLPRKLYDDIC
                     ALCER"
     gene            complement(6365..7138)
                     /locus_tag="VF226_04405"
     CDS             complement(6365..7138)
                     /locus_tag="VF226_04405"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7073260.1"
                     /translation="MKFARTASLCVLIAGAGVIWATPRASAQSQWVGNSPVFQSDIER
                     RQRQLEAERAERRKTYNVVYPRYMDGGERPEIKPKAPPIVYLEKNEKPGTIIVDTGGR
                     QLYYVLPGNRAYAYPISVGRDGFTWTGTERVSRIASWPSWTPPPEMRRRQPGLPITVS
                     GGLLNPLGAKALYLGNTIYRIHGTNNERSIGRASSSGCFRMMNEHVVHLASLARVGTV
                     VRVVANYSGVSTSPPLSALFSGLGFGFGEEVSSKKSRTN"
     gene            complement(7296..7973)
                     /locus_tag="VF226_04410"
     CDS             complement(7296..7973)
                     /locus_tag="VF226_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7073261.1"
                     /translation="MRKRIKRIATSLLIKNGFRGTSYGDIAAKLGITTTNIHYHFGPK
                     KRLVEVVVHDYVEATLARHREIWLHPEWTLPEKLRGVVEFNSERHRRFNRGGIGGRPW
                     SLIGRLRLESDLLSPSAQAALSRFTAELAGYVRIAVERAHEKGELRPDSPLDDIAFLL
                     ANIVNTSSTFTQDAGSIQRLAAFFETVSRVLTSAYAVSPPMQQASKGSTNRPEQIVPP
                     AKPSALV"
     gene            complement(8018..8521)
                     /locus_tag="VF226_04415"
     CDS             complement(8018..8521)
                     /locus_tag="VF226_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX7073262.1"
                     /translation="MTAKGETHAGRLLPAGEYWYEDVAVGDYFTTAGVTVTEAHVVGF
                     AGLVGDLFDVHMDEEFARSQGFPGRIAHGLLGLALADGLKTRCPVRLMGVATLSWNWS
                     FRAPILIGDRISAEIRIEDRRRTRRGDRGILKLGMKVKKQDGSAAQEGYSELLIRCRS
                     TAGEASD"
     gene            8712..10340
                     /locus_tag="VF226_04420"
     CDS             8712..10340
                     /locus_tag="VF226_04420"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7073263.1"
                     /translation="MRVITADEAARLVCDGDTVLVGGSGGGHAVPETLMAALGNRFRT
                     TGAPRQITSLHPVGLGDRATRGAGHFAQEGMLKRIICGTFVDSPPISEMAAADKIEAY
                     TLPQGALSQLMREMASGRPGLFTKTGLHTLVDPRHGGGKQSKRTTEDLVELVTLKGEE
                     WLFFKPFKVDVAFLRGTTADEDGNVTMEQEAIYGEMLSMAQATRRSGGVVIVQVKRLA
                     NRGTLPGKQVKIPGMLVDFVVVDPAQRQTYVTEYSPSYAGELREPLSTFEPLPLDPRK
                     VVARRGAMELFPGAVCNLGSGISTGIGTVGAEERILDEIVLTNEQGLIGGAPAIGKEA
                     GAARNFWAMIDQPYQFDFYDGGGLDLAFLSFAQVDQRGNVNVSRFDGKIVGIGGFVNI
                     SQNARKVVFGGTFTAGGLDVSWPGGRTRITREGRHRKFLSEVEQLSYNGDYGWERGQE
                     VLYVTERAVFRRVADGLELIEVAPGIDIERDILAHMGFKPRIAENCREMDSRLFRPEP
                     MGLAKDISAKPRRYRSERVHAWLQDREAIRAAAE"
     gene            10433..11437
                     /locus_tag="VF226_04425"
     CDS             10433..11437
                     /locus_tag="VF226_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073264.1"
                     /translation="MPVNTRRSFLAIVGLGLTAVAATPVLAQTKITLRLGHDQVTAHT
                     YHRTTQHFAKRVAEETNGEVQIRVMPGAQLGTETSMLDGLRSGNIDLSVSTTANASSF
                     VKKYGLLSVSYLFTDDKHFLRTLNDPEFLKMFDQMTAEANPGFRRAGLITPGARHYYS
                     NQGPVKSIEDLSQIKMRVMASPVESKVWGTLGTSPLAIPFGDLYTGMQTGLIQSAENS
                     PGSYALNKHYEVAKYFSLTGHQWPISGLFISDVTWNKLSDKHKAAIQKVGSEISTFSV
                     NDAVESDAKVLEDMKTKYGVIVNEVDTAPFVEKLSALQDEVAKELGVTDMLARIRALK
                     "
     gene            11533..12045
                     /locus_tag="VF226_04430"
     CDS             11533..12045
                     /locus_tag="VF226_04430"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073265.1"
                     /translation="MERFFRFNAALEQICGVAAALCLFVFTIIVAIDVFFRQVIATPL
                     LWPSEISVSLFVWSVLLGAAVCTRRNSHLVVEILPSLPTRIDWLLRLLVDLLCLIFAL
                     LVLWTGVKQAWAGISRYTPMMGYPLWPFLVALPAAAVPMVLFSIERLVRGSPPGSAGG
                     ATAGEEGSAA"
     gene            12042..13334
                     /locus_tag="VF226_04435"
     CDS             12042..13334
                     /locus_tag="VF226_04435"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7073266.1"
                     /translation="MSPILILFLSFFFLVILRVPIAHSLVLSSAFVIWLVGLSFDLIA
                     QQMFHGLNSFTLLAVPFFLLAGQLLNVGLITDRLVRLAYAMVGWVRGGLAHINVVVSM
                     IFAGMSGSSTADTAGVGAVMIPAMVKRGFDRRFTVAVTAASSTMGVIIPPSILMVIYG
                     AAGGVSIGALFLAGAIPGMLIGLGQMAYCYYYAVQHDMAAEQRASLSEFWAALKAGFL
                     PLLVPVIIIGGVLTGEFTATEAGMIACVYTVFLIFGIFRSAKWRSMPKVLTEAVVIYS
                     QPLLAVAGAMVFGWLLAYFDAPAQVAELSGPVAKDPTLALLFICAVFIVLGTFMDGIP
                     AIIIFMPIVQELVRTSGASDIHTGLVVVMTLALGLVTPPYGLCLLLASVIGNIKISEV
                     MGQIVIFYALFLAIVLLIIFVPEIALFLPRLVMPQFVN"
     gene            complement(13703..14713)
                     /locus_tag="VF226_04440"
     CDS             complement(13703..14713)
                     /locus_tag="VF226_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7073267.1"
                     /translation="MMSWKTLAGAVLAIAALGASAAEAQKVLKYAHFQPAKDDQPKHV
                     AALAFKEHVEKATNGSIKVEIYPAGQFGKDQPTMEGLKLGTLELAVAHDGAIATVYKP
                     IGVLGIPFLYENHEHAWRVYDSAWLKEISDDMVKKTGIRMLGLADNGVRHFTNSVRPI
                     KSPADMKGLKIRIQPSPVYQALVESLGASASAIPWADLPTALQQKVVDGQENGVTNIL
                     AASLYQHQKYITLDGHVWSVHAYLINDRFYHGLTDAERKAVDEGAQKAIAIHRKMTSD
                     QDKNAKAILEKVGMEVTELSPAEVAEFRKLAQPPVKAWAEKEIGKSYVDALFKAIEET
                     HN"
     gene            complement(14732..16021)
                     /locus_tag="VF226_04445"
     CDS             complement(14732..16021)
                     /locus_tag="VF226_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7073268.1"
                     /translation="MTTILATFFVIGLVIGVPVAFVMGIAGFLALAYEGHLSLLSVPQ
                     RFFDGINSFPLLAVPFFILAADLMTASGITTALLRFSNSLVGHIRGGLGHVNVLTSIF
                     FAGVSGSALADAAGPGAIEMRMMAKSGYDKYYSAALSAATATIGPIIPPSIIMVIYAL
                     TDNRVTVAGLFLAGVVPGLLLGAALMIANHVISVKRGYGTAQAHATWRDRLVALWKAI
                     PVLIMPAIILVGILTGLFTPTEASAVAVFYALFVGLFVTRTLNGPLIAKVLLQSGLVT
                     SAALLIVSMASLFAWLLTLMQVPQTLGSMIGTLTSDPTAILLIVAVFVLICGLFIDTL
                     PAVIILVPVLAPLTDQFGLNPLHFAMTIVLNLTVGMITPPVGAVLFVMTSVGRLRFDL
                     LSKAILPLLAAELFVLLLVVLFPALSTTVPGWFGYSR"
     gene            complement(16027..16530)
                     /locus_tag="VF226_04450"
     CDS             complement(16027..16530)
                     /locus_tag="VF226_04450"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073269.1"
                     /translation="MLSRAEQLLVWANRAAVIALMGSMAILVFVNVVSRYIFNHSIIW
                     VEEVTQYEMIWVTYLGAGLALREGRHVALDSLQDLLPQSLRRMTRIIVWIGMFAFLIA
                     LTVLGFMMSAFTWEHETPVLNMRAGIPYLAIPIGALLFALHLVLIARDFIEKRFEHVE
                     DLEAYAE"
     gene            complement(16828..17100)
                     /locus_tag="VF226_04455"
     CDS             complement(16828..17100)
                     /locus_tag="VF226_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073270.1"
                     /translation="MTTKALIIAATAAAAIVGLAAPDAAEAKRSRGKDVIEKHYITDG
                     PVKGFSGFAGGYYCDYRRVPNRKCTLLPNGQEKCVIVNWTLTHACY"
     gene            17276..18001
                     /locus_tag="VF226_04460"
     CDS             17276..18001
                     /locus_tag="VF226_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073271.1"
                     /translation="MSESMLSKVAIGVAVLAVIAAIWQYSAASSADQRAIAAEAARSA
                     AESQLKEAVAAREASEKALQEANAQAEQAKKDAEAQIASAKEAQAAAEQNLKDAQAQL
                     AEAKAARETAEAKSREAAEQVAAAQAAQKAAEAKAQKLADEVAQLKAAQETAANEAKA
                     ALQKAEADLNAAREQAKEAETARAAAEQSVQELRAQLAAAEAARDGAIREAKEIGDLL
                     AKEREARQAAERSRQSAPQPVAQ"
     gene            18168..18662
                     /locus_tag="VF226_04465"
     CDS             18168..18662
                     /locus_tag="VF226_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7073272.1"
                     /translation="MTDTHPAAAFPSPRHDHAPCVALALARAERAFEEKGLRLTDLRR
                     RVLEEIAGSHHAIGAYDVLERLSRKGSRLAPISVYRAIDALLSAGVIHRLESRNAFFA
                     CHATHTADRDQIVLACERCGRVAEVPGKPVFDAISSIAKKAAFKPVHTVAEVSGVCDD
                     CQAR"
     gene            18679..19581
                     /locus_tag="VF226_04470"
     CDS             18679..19581
                     /locus_tag="VF226_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX7073273.1"
                     /translation="MNILPINAASDLGHEGRWSHDHAHPHHHAPNEASTLGLAPRVQD
                     ALISARGLWLTRSGRTILEDIDLDIGRGEIVTLIGPNGAGKTTLVRVLLGLTSPDRGE
                     IRRSPGLTIGYVPQRFDVDRAIPMTVARFLALGRSGRPERVRAVLAEVGASRVLEQQI
                     VSLSGGELQRVLLARALLRDPNLLVLDEPVRGVDYAGEAELYTLIGRLRTARGLSVLL
                     VSHDLHIVMAQSDRVVCVNRHICCSGIPEAVAKDPSYARLFGAHAARAFAIYSHQHDH
                     HHDLAGETRPAAPDAADGPGRQEA"
     gene            19586..>20181
                     /locus_tag="VF226_04475"
     CDS             19586..>20181
                     /locus_tag="VF226_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7073274.1"
                     /translation="MDPFLIRAFVAGIGVAILAAPLGCFVVWQRMAYFGEAVAQASLI
                     GVALGLAIQADLTASALVVSAVMALLLIVLSRQSLVPLDSLLGLLAHAALAVGVISAS
                     LVQGPAVDLLGYLFGDIFAVSAGDLWWVFGGGAVILAALAWLWQPLLSIAVHEELAAA
                     EGIPRDLVKGVFVVLLALAIAIAIKIVGILLAIAFLIMP"
BASE COUNT         3938 a         6143 c         6125 g         3975 t
ORIGIN      
        1 cggtctggcc attgcgtctg tcacccggga ggaaggcctc gacttcgtgc ctttgcattg
       61 ggagcgctat gatctcgtcg tccgcagagt cgaatatttc gagccaccgc tgcaacgcct
      121 gttcgccttc acgcgcaccg aactcttcag cgagcaggcc aaagcgttgg gcggttatga
      181 tattgctcag accggaaccg tggttttcaa cccgcggcga tgatccgaaa agccatagct
      241 cgccaatccg ccggtcagcg gccaaagatc atgttcggga cgatcaggac aatacctggc
      301 acgaagatga ggaccagcgt gaccaggctc accgcggcga acagcgggat cgattcgcgc
      361 gtatactcgc ctatgggcac attcaagatg gagcagacgg catacatcgc agcgccgccg
      421 cccgtcccag gacgaatacg acattgaagt gaattggatc gcaccgagct gacggatgag
      481 cggcagaaaa atcggcgtca gcataaatga tcagcactgt cgcgtcgatg tagaacccgg
      541 cgatgaggtc tcagccgctg gcgcgcggct tatagctgcc cgcaggtgtt cggaagggaa
      601 tggccaatct gttccagcca ttgatcgtaa tgatggccaa agtcaggtcg acgatctcct
      661 tctccgagaa atactgctga agctccgtgt aaagagcatc ttcgatggga tgctcagaaa
      721 tttttgtaat cgcctcggtc caggcaagtg ccgcacgctc gcgctcgctg aagaacggag
      781 tttcacgcca agccgacagc gtgtagagcc gttgctccga ctcgcccgct gcgcgagcgt
      841 ccttggtgtg catgtcgaga cagtaggcgc atccgttgat ctgggaggcg cgggttttga
      901 cgagctcgag caacgatcgc tcaaggccgc atccctcgac gtagtcattc agatgacgga
      961 gagcggcgaa gccctgtggc gataccttcc aataatccag tctcgatttc atcatgctcc
     1021 tccgttcgtt tgctgcaggc tggggcctgt cgaactcgat agcgatggga tcgctggccg
     1081 gaaaggtctc ggctagcgcc ttatccagga gttcgtcgag atgggatcgc gaagaggtgt
     1141 tgctggcgac tcgttccatc ctgctcttgt tgtcgggcat ccgcaacctc ttggaggttc
     1201 tctatgctgt actggctaca ccgtcggcat gcatatagac ccattggcct gccggtggtt
     1261 ccaagtggaa ccaatctgta gcgcgccgct ctgggcaatg tgagatcaat gcccggcagc
     1321 ctccatcgca tgccgcagaa cccgggcgta attgccaatg gcgattttct gcagccgaat
     1381 ccggtccaga cctcgtcttt ccatgtacct cactgcacgt cgcaggtcgg cataagtcga
     1441 aatcggtgat ccggagagcg cattcatgtc ggtgccgaag gcgacgtggt catctccgag
     1501 ccaagctgcc atctcgatca gtcggtccgc ataggcctcg acggttgctc cgacgtcgct
     1561 gcgcaacgcc caaagtccga tgacgccccc tttggaggcg atcgcgcgcg cttcgtctat
     1621 gccaagtcgc cgagcttgca acggccccag cttgcggcgg ggcacgtcgt tcctggccac
     1681 cgagctgtgc gaccacacca ctggcgtacg tgacgtttcc aacgcctgcc gtacggcggt
     1741 tgccgtgctg tgtgcgagat ccacgaggat gccgagtcgg ttgcactcct caatcacacg
     1801 gcggccgaac gccgtgagcc cgccatgcct gggagcttcg gtctggaaat caccgatcga
     1861 attgcgcacg taatgaacga gctggatgtg ccgaatgccg gcgtcatgcg caggcttcag
     1921 gccggctatg ccgtcgtcga gaaaacttgc gccttcgacg gagaggacga cgtgcggctc
     1981 acccgtcagc gcgcggtcaa ggtcggcggg cgtcaatacg atcttgagac cctgttcagc
     2041 gatatgggat ttggcgcggg ccagttcggc ctcgagccat gtccatgcct cgccaggttt
     2101 gggcgttgcc gactgacgca gcccgccggc gccgggtcgt atccagggca tatcgccgat
     2161 gagagacaac gagacgagcg aggcgcgtcc gctcgccata ttccgcccga gtggcagcaa
     2221 agaaggcttc gggccgacaa aaaaatgatg cgcatgcata tcaccgatcg gtggcgatcc
     2281 atcggcgcac gcaggaattg gccggaaccc cgccaccatt ccggtggcga ggccggttag
     2341 cactgtacgg cggttccaat cgagcatcac gcgcaagtca gaagaggagc gatgatgcca
     2401 gatcggggca gccacaatgc aaatcgccat ccgatcattc aattactcga acatgatcgt
     2461 ggcgacataa tccatcgttc gcgttctgtt gtttggcggc agcagcgtat tgatgagata
     2521 agaggtcggc gagtagttgt ccggtatggc ccttcggaat gcattcccaa ggaaaaatgc
     2581 ctggatgcga tgaaatccgc cttcggccgc cgccttataa tacctttcca gcggagtgaa
     2641 tttaggctgc attgcctggg ccacctcgcg atgcccggag gacatgttgg ccagtttgga
     2701 agggttttcg acgaatacgt tgtgcagaag cgcattccgt tccgcatccg gcagtttcat
     2761 ggcatcgatc accagaagcg gcccatcggc aagtcccgca tgcgatgatg cactccggcg
     2821 gctgatgtag ttgcccgtgg ctttcatggg agtcatcggc gggtcgacgc acgatggcac
     2881 gcccgtgcca ttgaaggact cggcagtcgt cttcggatca catttgcgca atgctagaat
     2941 ggaccagacg aactccgagc agaacatgtc cgtcggcacc acgtcgtcat gcccgagcgc
     3001 cgacctcgcg acattcttca cgaaatcgag cggcttgccg ggcgagtact ttggcgcgtt
     3061 gtaatcagtg ttgaatgaaa tctgctttgg ataaatccgc ttggcaccac cgcccagcct
     3121 cgtggcccac gcgaggatat tggcgcgttc ggcatcggtc agcccgcgtg ggcggatcac
     3181 atgaatgaat ttgagcttcc gataatgatc accgctgaaa tcacctttca gccttggacc
     3241 aagatgttcc gcgtccatcg ggttgtcgag ctggtagact gccccgtcct tcacatatgc
     3301 gaggccggcg tgactgacgc ccatctggag gttcggatag gtgccagcgc cgccccactc
     3361 tggcctgaac gagagaagga tgtcgcctgt ctgcagcaga ccgctcttgg ccatgcgctt
     3421 tgcaaagccg gagcgcttgg cgatgaccag tctgaggccg atgccagcag agtttccgtt
     3481 ggcttcgggg tgcttgaatt caacgaggcg gtctgacgta ttgaacgagg gcggcagcgg
     3541 cgcgcgagcg ctgtccggaa cagcaaaata gactgggatg ctgagcttct tcgcgatctc
     3601 ctggttcacc ttctcattgt aggtgaactc ggccgcactg agaggtaggt ggccggcagc
     3661 ccatgccact gcggcgatag cggcgaacgt caaggaacgc atgttttcag atcctccccg
     3721 cgggtggtgg cttgccttgc aaacgatgtc catttagcac gttacccccg gcggccgcac
     3781 ctgatcagat gtcgtcccat ccacaactag agcgtgaacg acgagccatg ccgaaacgga
     3841 gagaaaggaa ggcgccgcgt aaatcgcgac gcggctggct cgtgctcgcg gcggttcctc
     3901 tcatggcatt tggctgcttt tcggtatgga tcgcctggct cggacgcgaa agtggacctc
     3961 cgagccgctg ctatggcggc gtcgttggcg gacgcctcga aggtggccgg cgactgccgc
     4021 tctggggaga gaactacagc gcctacagca cgttgggata tctcgcgggc cggaccttca
     4081 tgcacgggac cgttcgcgac gccatactgg ctgcctacgc ggagctgaac gagactcacc
     4141 ctgacttgcg ctacgtgtac gccgagagcg gatggccctg gggcgggcct ttttcgccgc
     4201 atcgctccca catgaacggc acgtcagtgg atttccacgt tcccgtccga actctcgacg
     4261 gcgccgtgtc ggagcttccc gcctcggtct tcaacaagct cggctatgcc atagaattcg
     4321 atcgatcggg gcagtggaac ggctacaaaa tagacttcga ggcgatggcc ttgcatctca
     4381 tcgctctcga tcgggcagca cgcgcaagtg gcatctcgct ccgtcgtgtt attttcgatg
     4441 ttcagctgca gccgaagctg ttcgcgacag aagccggccg gccccttcca aaggtaatga
     4501 gattcaacca gcggcagtcg tgggtgcggc acgacgagca ctaccacgtt gatttcaatg
     4561 tgccctgccg gtgaggtctc gcgcgcccac attgcgagag atcggccgga aagcacctgc
     4621 tgatgttgca gcgaggaaat cccacggcgc cttttcggtc gccaacgcgg gcatgtgccg
     4681 gataggcacg agattgggcc gcttccgcgc accgacgctg gtcgtccagg aaggcggatg
     4741 ttccaggcgc aatctgcggt caggttcgag ggcattcttc tccggtctgg caagtgcatg
     4801 gtacgaccgg tgacgggcaa cggtacctat ggtcctgagg cgatcgacca ggccaactct
     4861 gcccgccgct ggtcgggatc aacccagtgc atccgctagc acccgagcgc aggtgccacg
     4921 acctaagcgc acatcccgcg agcctagccc gccccggcaa tctgtcccat actggccagc
     4981 gtcttcaaat acgtagggaa acggaagcga tcgtgactga aaacgtgacc atcacacttg
     5041 atgagttgtg gaggctcggc atagaggttc tggaggcgaa cggcttctcc aaggatcatg
     5101 cccgcaccat caatgcgtcg atctgggcct gtcagcgcga cgaatgccac tctcacggcg
     5161 cctatcggct gatttcctgc gtcgaaaccg ttcgcgccgg caagatttca ccggttgccg
     5221 agcctgaggt ctcgcaccct gctcccggca tcgtccggat cgacgcaaag ggtgcctatt
     5281 ccctcctttc gttcgaagtg ggccgaccgc tcctcgtcga gaaggcccgc aagcaggggg
     5341 tcgcggttct ggccatcaat cactgctatc atttctccgc cctttggccc gaggtcgaag
     5401 cactggccaa ggatggcgtc gcggccatcg cgatgacacc gggcacgagc actgttgctc
     5461 ccgcaggtgg aacgaaacct gttttcggaa ccaatccctt cgcatttgcc tggccccggc
     5521 cgaatggcaa cccttacgtg ttcgacttcg cgacgagcgt cgtcgctcgg ggcgagatcg
     5581 aactccacaa gcgcgcagga aagccaatcc cgaccggctg ggccatcgat gcaaacggag
     5641 aaccgacgac ggacccggcg cgtgctctgg aaggcgcatt gctcccgttc gggtcctaca
     5701 agggcacggc gatcgcgacc atgatcgagc tcctcgccgg cccgctgatc ggcgacctgc
     5761 tgagcccgga ggcgaaggag tatgacgccg gcgccaatgt cacgccgtac cggggtgagc
     5821 tggtcatcgc cttcgatccg gtttcctttc tgggcgcgga agcggatcag caccttcaga
     5881 gagccgagcg gattttcgag gagatcacca gccagggcgc acggctaccc tcacagcgcc
     5941 gatatgaggc tcgcgcccga agtcttgcat cgggcacagt gactctgccg cgcaagctct
     6001 atgacgatat ttgcgcattg tgtgagcgat gagcgcgatc caacgatgat atgagagggc
     6061 cgggtggggg tttcaaagga cagcccctcc ccctccaggg aaaaggagaa tgggcacagg
     6121 ctcaactaag cttgtgccca ccttttattg agaacgatcc agaattcaga ggctagagca
     6181 tttgcgattt gacagaagcg cgccaagcca agctctcgcc taatctcgct ctccccagcg
     6241 gcgagacggt cggggtgcag gttccgccgg tcacagatgt tgtagttcct cacccgactc
     6301 ttcggacaag ggagaggtta acggcgtgct tgctgttggc gtttccacct gaatcggaaa
     6361 agctctagtt cgttctgctt tttttcgagc tgacctcttc accaaatcca aaaccaagtc
     6421 ccgaaaaaag cgcggacagc ggaggggacg tgctcacgcc cgagtaattc gcaacaacgc
     6481 gcacgaccgt accgacgcgc gcaagcgatg ccagatgcac cacgtgctcg ttcatcatcc
     6541 gaaagcaccc cgacgaggac gcccggccga tcgatcgctc attgttcgtg ccatgaatgc
     6601 gatagatcgt attgccgagg taaagcgctt tcgctcccag tggattgagg agaccgcctg
     6661 acacggtgat cggcaggccc ggctggcgtc gacgcatctc gggcggaggt gtccacgacg
     6721 gccacgacgc gatgcgactg acccgctcgg ttcccgtcca cgtgaatcca tccctaccga
     6781 ccgagatcgg gtaggcgtat gcgcggttgc cgggaagaac gtaatagagc tgccgcccac
     6841 cggtatcgac gatgatcgtc cccggtttct cattcttctc caggtagacg ataggtggcg
     6901 ccttcggctt gatctcgggc cgctcacctc cgtccatata cctcggatag acgacgttat
     6961 aggtcttccg gcgttcggcg cgctccgcct cgagctggcg ctggcgccgc tcgatgtcgc
     7021 tctgaaaaac ggggctattc cccacccatt ggctttgcgc cgatgcccga ggtgtggccc
     7081 agatcacgcc ggccccggcg atcagaacgc agagtgatgc agtccgagcg aatttcatgg
     7141 ctcaccccga aatgtccctc ctcaccaaac gaggcggcgc agcagtgttc taaaattcca
     7201 accagaacaa gtaatctaat gcggccagcc gggacaagct tgcctcgccc gctgacgccc
     7261 ggccagccaa aaaatcaggg ctcagcgtgc caattttaca ccagggccga cggttttgcg
     7321 ggcggcacga tctgctcggg tcgattggtc gaaccctttg aagcctgttg catgggcggg
     7381 gacactgcat atgcggacgt cagcaccctc gaaaccgttt cgaagaaagc tgcgagccgt
     7441 tggatgctgc cggcatcctg agtgaacgtc gatgatgtat tgacaatatt ggccaacaag
     7501 aacgcaatat catcgagtgg actgtcgggc cgaagctcac ccttttcgtg agcgcgctcg
     7561 acggcaatgc ggacgtagcc ggcaagctcg gccgtaaacc gggaaagcgc cgcttgcgcg
     7621 gacggactta gcagatcgct ttcaagcctc agtcgcccga tcagactcca cggtcggccg
     7681 ccgatgccgc cgcggttgaa cctgcgatgg cgttcgctat tgaattctac gacgccgcga
     7741 agcttctccg gaagcgtcca ctccggatgc agccagattt cgcgatgacg tgcgagcgtc
     7801 gcttcgacgt agtcgtgtac gaccacctcc accaaccgct tcttcggccc gaaatggtaa
     7861 tggatgtttg tggtcgtgat gcccagcttg gccgcgatgt cgccatagct cgtcccgcga
     7921 aagccgttct tgatgaggag gctggtcgcg atcctcttaa ttcttttgcg catttggtcg
     7981 ctctgcgcgt tgcctggcga agcgcccatg agtcacctca atcggaagct tcgccggcgg
     8041 tcgagcgaca tcggatgagc agctcgctgt agccctcctg cgctgcgctg ccgtcctgct
     8101 tcttgacctt catgccgagc ttgagaatgc cgcgatcccc ccgccgtgtc cggcgccgat
     8161 cctcgatgcg gatttcggca ctgatgcgat ccccgatgag aatcggcgcg cggaatgacc
     8221 agttccaact tagggttgca acacccatga gccgcacggg gcagcgcgtc ttcaggccgt
     8281 ctgccagcgc caagccgaga agcccatgag caatgcggcc cggaaagccc tgtgaccgag
     8341 cgaattcctc atccatgtgg acgtcgaaca ggtcacctac caggcctgcg aaaccgacca
     8401 cgtgggcttc cgtcaccgtg acacccgcag tcgtgaaata atcgccgacg gcgacatcct
     8461 cgtaccagta ttcgccggcc ggcagcaggc gccctgcgtg agtttcgcct tttgcggtca
     8521 tcgttcgcag tgccccctcc tcaagggtga tgcaatcgca atccggatcg ctcgaggcaa
     8581 catagactta cttactactc aataaaagac gattgactag aaaactgccc ttggccagga
     8641 tgtagaggga agctggcagc aacagctacg gatggcgggc aagtttgcag cgctgacggg
     8701 agagagcagt cgtgagggtg attacagcgg acgaggccgc gcgcctcgtg tgcgatgggg
     8761 acacggtgct ggttggaggc tcgggcggcg gccacgcggt gcccgaaacc ctgatggcgg
     8821 cgctcggaaa tcgcttccgc acgacaggtg cgccgaggca gattacctct cttcatcctg
     8881 tcggactggg tgatcgggcg acacgtggcg ccggacattt cgcccaggag ggcatgctca
     8941 agcggatcat ctgcggcaca ttcgtcgact ctccgcccat ttctgaaatg gcagcagcgg
     9001 acaagatcga ggcctatacg ctgccgcaag gggccctctc gcagctcatg cgcgagatgg
     9061 cctcgggcag gccaggccta ttcaccaaga cgggcctgca cacactggtc gatcctcggc
     9121 acggcggcgg gaagcaaagc aaacgcacga cggaggacct ggtcgagctc gtcacgttga
     9181 aaggcgagga gtggctgttc ttcaagccgt tcaaggtgga cgtcgcgttc cttcggggca
     9241 ccacggccga cgaggacggg aacgtcacca tggagcagga ggccatctac ggcgaaatgc
     9301 tgtcgatggc ccaggccacg cgccggtccg gaggcgtcgt gatcgtgcaa gtcaagcggc
     9361 ttgccaaccg cggaacgctt cccggcaagc aggtgaagat tcctgggatg ctggtcgatt
     9421 tcgtcgtcgt cgatccggcg cagcgccaaa cctatgtaac ggaatattcc ccgagctacg
     9481 cgggcgaatt gcgcgagccg ctctccacgt tcgagcccct gccgctcgac ccgcgcaagg
     9541 tcgtggcgcg acggggggca atggagcttt ttccaggcgc ggtctgcaat cttggctcgg
     9601 ggatttcgac cggcataggc acggtgggcg ctgaggagcg catcctcgac gagatcgtat
     9661 tgaccaacga gcagggcctc atcggtggcg cgcctgcaat cggcaaggag gccggtgcgg
     9721 cgcgaaactt ctgggccatg atagaccagc cctatcagtt cgatttctat gatggcgggg
     9781 gtctcgatct cgctttcctg tcctttgctc aggtcgacca gcgcgggaac gtcaacgtca
     9841 gccgcttcga tggcaaaatc gtcggcatcg gcggtttcgt aaacatcagt cagaacgcgc
     9901 gcaaagtcgt attcggcgga acgttcacgg ctggcggact agatgtttcg tggccgggcg
     9961 gacggacccg catcacgcgc gaaggcaggc atcgcaaatt cctgtccgaa gtcgagcagc
    10021 tttcctacaa tggcgactac ggctgggaac gtgggcagga agtgctctat gtgaccgagc
    10081 gcgccgtttt ccgccgggtg gccgatggcc tcgagctcat cgaggttgca cccggcatcg
    10141 atatcgaacg cgatatcctg gctcatatgg gattcaaacc gcgtatcgca gagaattgcc
    10201 gcgagatgga ttcgcgcctc ttccggcctg agccgatggg gctggcaaag gatatatcgg
    10261 ccaagccgcg tcggtaccgc tccgagcgcg tccatgcctg gctgcaagat cgcgaagcta
    10321 tacgcgcagc agcggaatag aggcgtgcgg caaaggttcg accgacacgc ctttgggcgg
    10381 agcaatcgct ccggtgaatc tgggaccgaa acaaatgaag ggagaagcgc ccatgccagt
    10441 caatactcgt cgctcattct tagccatcgt aggcctcggt ctgacagcgg tggcggctac
    10501 gccggtgctt gctcagacca aaattacgct tcgcctgggt cacgatcagg tcaccgccca
    10561 cacttatcac cgcacaacgc agcatttcgc caagcgcgtc gccgaggaga ccaacggcga
    10621 agttcagatc cgcgtcatgc cgggcgcgca gctgggcact gaaacgtcca tgctcgacgg
    10681 actgagaagc ggcaatatcg acctgtcagt atctaccact gccaacgcat cgagcttcgt
    10741 caagaaatac gggctactca gcgtcagcta cctgtttacc gatgacaagc atttcctgag
    10801 aacgctgaac gaccctgaat tcctcaagat gttcgatcaa atgactgcgg aggccaaccc
    10861 cggcttccgt cgggctggac tgatcactcc tggcgcacgt cactattaca gcaaccaggg
    10921 tccggttaaa tccatcgaag acctcagcca gatcaaaatg cgtgtgatgg cttcaccggt
    10981 cgaatcgaag gtttggggca cgctgggcac atcgccgctg gcgattccgt tcggcgacct
    11041 ctacaccggc atgcagaccg gactcatcca atctgctgag aattcgccgg gctcctacgc
    11101 gttgaacaag cattacgagg tcgccaaata cttctccctg accgggcatc agtggccgat
    11161 ttcggggctg ttcatcagcg acgtgacctg gaacaagctt tccgacaagc acaaagctgc
    11221 catccagaaa gtcggaagcg agatctccac tttctcggtc aacgatgccg tcgagagcga
    11281 cgccaaggtg ctcgaggata tgaaaacgaa gtacggcgtc atcgtgaacg aggtcgacac
    11341 ggccccgttc gtcgaaaagc tttcggcgct gcaggacgag gtggccaaag agcttggcgt
    11401 aaccgatatg ttggcgcgga tacgcgctct caaatagctc cgcctgatca ggcgcagtct
    11461 gcgaaagcgt tctacgctcg agtttgtcgc ttacgattgg gccttgctgg cagggcagca
    11521 cggaggaaat gaatggagcg cttcttccgc ttcaatgcgg cgcttgagca aatttgcggc
    11581 gttgccgctg ccctctgttt gttcgtattc acaatcatcg tggcgatcga cgtgttcttt
    11641 cgacaggtga tcgccacccc tctactctgg ccttccgaga tatccgtttc cctgttcgtt
    11701 tggtcggttc ttctgggtgc ggcggtctgc acacgaagaa attcgcatct ggtggttgag
    11761 atactgccgt ccctgccaac ccgtatcgac tggctgctgc gccttcttgt cgatctcctc
    11821 tgtctcattt tcgcgctgct ggtcctatgg acgggtgtca agcaggcctg ggccggaatt
    11881 agccgctaca ctccgatgat gggctacccg ctctggccgt tcctggttgc tctcccggct
    11941 gccgccgtac cgatggtcct tttctcgata gagcggctcg tccgcggctc gccgccagga
    12001 tcagcaggcg gcgcaacagc gggtgaggag ggatcggcgg catgagtcct attctgattc
    12061 tttttctcag tttctttttt ctcgtcatct tacgggtgcc gatcgcgcat tcgctcgttc
    12121 tgtcatcggc gtttgtgatc tggctggtcg gccttagctt tgatctcatt gcgcagcaga
    12181 tgttccatgg actcaactca tttacgctgc tggccgtgcc attcttcctt ctggcagggc
    12241 aactcctcaa tgtagggctg attacggatc gcctcgtccg cctcgcctat gccatggtcg
    12301 gctgggtgcg cggcggcctc gcgcacatca atgtcgtcgt cagcatgata tttgccggca
    12361 tgtccggatc atcgacggcc gacacggcag gcgtcggcgc ggtaatgatc cccgcaatgg
    12421 tgaagcgggg cttcgatcgt cgcttcacgg tggctgtcac agccgcctcc tcgaccatgg
    12481 gcgtcatcat cccgcccagc attctgatgg tgatctacgg tgcggctggc ggcgtttcca
    12541 ttggcgctct atttctggcc ggggctatcc ctggcatgct gatcggcctt ggacagatgg
    12601 cctactgcta ctactacgct gtccagcacg acatggccgc agaacagcgc gcgtccctga
    12661 gtgaattctg ggcggccctc aaagcaggat ttctccccct tttggtgccc gtcatcataa
    12721 tcggcggtgt gttgaccggc gagttcaccg caaccgaagc gggcatgatt gcctgtgttt
    12781 acacggtttt tctgattttc ggcatcttcc gatcggcaaa atggcgatca atgccgaagg
    12841 ttctgacgga agccgtcgtc atctactcac aaccgctgct ggcggttgcc ggtgccatgg
    12901 tcttcggctg gcttcttgcc tacttcgacg cgccggcgca ggtggccgag ctttcaggtc
    12961 cagttgcgaa ggatcccacg ctggccctgc tcttcatctg cgccgtattc atcgtgctcg
    13021 gaacattcat ggacgggatt ccggccatca taatcttcat gccgatcgtc caggagctcg
    13081 tccgaacctc tggcgcgagc gatattcata cagggctggt cgtcgtaatg acacttgctc
    13141 tcggactcgt tactccgccc tacggacttt gtcttttgct cgcatccgtc attgggaata
    13201 tcaaaatctc cgaggtgatg ggccaaatag tgatattcta cgccctcttc ctagcgatcg
    13261 tactgctcat cattttcgtt cccgagatcg cgttgttcct gccgcgcctg gtcatgcctc
    13321 aattcgtgaa ttgaaccgga tcgataactt gagtattgcg caaacgctcc cgcgcttgcg
    13381 tcagctcatc acattccgat ttcgtccgcg gcttactggt acaagacgcc ggtctctggt
    13441 tcgagttcat caacgtagtc gcacggcatg accggctaga gcattccgat ttggacagaa
    13501 gctcgccaag tcaagctcgc gcccaatctc cctctccccc gtgtggagag ggtcgaggtg
    13561 ctgaccctca ccgggccctt cgggccgccc tctccctatg agaggtcaac gaagtgtttg
    13621 ctgttggcgt ttccacctga atcggaaaag ctctaatcgc gctcggagca gtggccgctc
    13681 cgagcacaga aatcttgttt ccctaattgt gcgtctcctc gatcgccttg aagagggcat
    13741 caacgtagct cttgccgatt tctttttcgg cccatgcttt gaccgggggc tgtgccagct
    13801 tgcggaactc agcgacctcg gcaggcgaga gctccgtcac ctccatgccg accttctcca
    13861 gaatggcctt ggcattcttg tcctgatcgc tcgtcatctt gcggtgtatg gcgatcgctt
    13921 tctgcgcgcc ctcatcgacc gccttgcgct cggcgtcggt cagtccgtgg tagaagcgat
    13981 cgttgatcag gtaggcgtga acgctccaga cgtgaccatc gagcgtaatg tacttctggt
    14041 gctgataaag actcgcggcg aggatgttcg tgaccccatt ttcctggccg tccaccacct
    14101 tctgctgcaa ggctgtcggc agatcggccc agggaatggc agaagccgag gcgccgagtg
    14161 actcgaccag cgcctgatat accggcgatg gctgaatgcg gatcttgagc cccttcatgt
    14221 cagccggtga tttgatcggc ctgacgctgt tcgtgaagtg gcggactccg ttgtcggcca
    14281 gacccagcat gcgaatgccg gtcttcttca ccatgtcatc agagatctcc ttcagccagg
    14341 cgctgtcgta aacacgccag gcatgctcgt ggttttcata gaggaaagga atgccaagga
    14401 cgccgatcgg cttgtatacg gtggcaatgg caccgtcatg cgcaacggcc agctcgagcg
    14461 tgccgagctt caggccctcc atggtcggct gatccttgcc gaactggccg gccggataga
    14521 tttctacctt gatcgacccg ttcgttgcct tctcgacgtg ctccttgaag gccagagccg
    14581 ccacgtgctt cggctggtcg tccttggccg gttggaaatg ggcgtacttc agcacttttt
    14641 gcgcttcggc cgcggatgca cccaaagccg caatggcgag aaccgctccc gccagtgttt
    14701 tccagctcat cataatgttt cctcctttgc tttagcgcga ataaccgaac cagccgggca
    14761 ctgtcgtgct cagtgcagga aaaagcacca ccagcagcag aacaaataac tctgctgcca
    14821 gcagcggcag aatcgccttg gagagcaggt cgaaccgcaa acgcccaacc gacgtcatga
    14881 caaacagaac ggctcccacc ggcggcgtga tcatcccaac agtgagattg aggacgatcg
    14941 tcatggcgaa atgcaaagga ttaagtccga actgatcggt gagcggcgcc aaaaccggga
    15001 cgagaatgat gaccgcggga agtgtgtcga tgaaaagacc gcagatcagc acgaagacgg
    15061 cgacgatcag caagatggct gtcggatcgc tggtgagcgt gccgatcatg cttcccagtg
    15121 tctgcggcac ctgcatgagc gtgagcagcc atgcgaacaa ggaagccatc gaaacgatga
    15181 gcaacgccgc agatgtcacg agcccgctct gcagcagcac tttcgcaatc agcgggccat
    15241 taagcgtccg cgttacaaaa agcccgacaa acagcgcgta gaaaacggct acggccgatg
    15301 cctcggtggg cgtgaacagg cccgttagta ttccgaccag aatgatggcc ggcatgatca
    15361 ggacgggaat agctttccaa agcgccacga gccggtcgcg ccaggtcgcg tgagcctgcg
    15421 ccgtgccata tccccgtttc accgaaataa cgtggttcgc gatcataagg gcggcgccga
    15481 gcagcagtcc aggcacaacc ccggcaagga acagtcccgc gaccgtcacc cggttatcgg
    15541 tgagcgcgta gatcaccatg atgatcgacg gcggaatgat cggaccgatc gtggcggtcg
    15601 cagcggaaag cgctgccgaa tagtatttgt cgtatcctga cttcgccatc atccgcattt
    15661 cgatcgcgcc ggggccggcg gcatccgcca gcgccgatcc gcttacgccg gcgaagaata
    15721 tgcttgtcag cacgttcacg tgaccaagcc caccgcggat atgaccgacg aggctgttcg
    15781 aaaagcgcag gagagctgtc gtgatcccgc tcgccgtcat cagatccgcg gccaggatga
    15841 agaacggcac cgccaacaat gggaacgagt tgatgccgtc aaagaagcgc tgtgggacgg
    15901 aaagcaaaga gagatggccc tcgtaggcca gcgccaggaa tccggcgatt cccatcacga
    15961 acgcgaccgg cacgcctatg accaaaccga tcacgaagaa ggtcgcaaga atggtcgtca
    16021 tgcgccctac tccgcgtagg cttcgagatc ttctacgtgc tcgaaacgct tctcgatgaa
    16081 gtctcgagca atcagaacga gatgaagagc aaaaagcagg gcgccgatgg gaatggccag
    16141 gtacgggatg ccggcgcgca tgttgagaac cggcgtctca tgctcccagg tgaaggcgga
    16201 catcatgaag cccaggacgg tgagggcaat cagaaaggcg aacatgccga tccagacaat
    16261 gatgcgcgtc atgcggcgga gcgactgtgg caggagatcc tgaagactat ccagggcgac
    16321 gtggcgacct tcgcgcagcg ctaggccggc acccaggtag gtgacccaga tcatctcata
    16381 ctgggtcacc tcctccaccc aaatgatcga gtggttaaag atgtatcggc tgacgacatt
    16441 cacgaaaacc aggatggcca tcgagcccat cagtgcgatg acggcggcac gattggccca
    16501 cacgagtagc tgttctgcac gtgatagcaa taggcgtctc cccgctgctg gccccccttt
    16561 gcgggcggga ggccttccta tttagccaaa cctagcagct ctggcgcgag cctcaatggc
    16621 agccgggtga cactagccgt gatcttgaaa atgaagccgc ttgcagagtt ggctcggcct
    16681 gccgtcgccg cgggagggac gtgcgcgttc gcccctcttt cgagtacgga gcggtcggca
    16741 ggcccgattc cggacctgca atgagcctat cctgcacgga gaacgggcag gccaggcatc
    16801 gcctgtcctg cccaactggg ccgaaggtca gtagcacgcg tgtgtcaggg tccagttgac
    16861 gataacgcac ttttcctggc cgttcggcag gagggtgcac ttgcgattgg gcacgcgccg
    16921 gtaatcacag tagtagccgc ccgcaaatcc cgagaagcct ttgaccggac catcggtgat
    16981 gtagtgcttc tcgatgacat ccttgccgcg agaccttttg gcttcagccg cgtccggagc
    17041 agcgaggccg acgattgcgg cagcagcggt agcggcgatg atgagagctt tcgtggtcat
    17101 ggcgtcctcc tcaggtgtga gcgtctggag ggcaccttat gtgctgagca gcccgcccgc
    17161 tgttccgccg gaaacaggtc cagtgcgatc cgcctcgaac cagttgtggg atgattttta
    17221 aggacgttag tcctagagtg ctcgcggaag gcgtgaagct ggggagtaac cgaaaatgtc
    17281 cgagtccatg ttgagcaagg ttgcaatcgg cgttgccgtc ctggccgtga ttgcagcgat
    17341 ctggcagtac agcgccgcat cttcggctga ccagcgggcg atcgccgcgg aggccgcccg
    17401 aagcgctgcg gaaagccagc tgaaagaggc tgtcgccgcg cgcgaggcat ccgaaaaggc
    17461 gttgcaggaa gcgaacgccc aggccgagca ggccaagaag gatgccgagg ctcagatcgc
    17521 aagcgcgaag gaggcccagg cggccgccga gcagaacctg aaggacgcgc aagcccagct
    17581 cgccgaagcc aaggctgcgc gggaaacggc ggaggccaag tcgcgcgagg ctgcagagca
    17641 agtcgccgcc gcccaggcag cccagaaagc cgcggaagcg aaggcacaga aattggctga
    17701 tgaggtcgcc cagttgaagg cggcccagga aacggccgcg aacgaagcca aagctgccct
    17761 ccagaaggcc gaggccgacc tgaacgccgc gcgcgagcag gccaaagagg ctgagacagc
    17821 acgcgccgcc gccgagcagt ccgtgcaaga gttgcgcgca cagcttgcgg cagcagaagc
    17881 ggcacgagac ggagccatcc gcgaggcgaa ggagatcggc gacttgctgg ccaaggagcg
    17941 tgaggcccgc caggcggccg agcgctcacg ccaaagcgca ccgcagcccg tcgcgcagtg
    18001 atcgcggcat aacttcttca ctagtggcag cgatagcagg ggcgcccgcc ggggcgccct
    18061 tttgcgtcgg gggcaccgga tgtgagcaag tgcacttcgg gttagattgc caagtgaaat
    18121 gtgataacgt aacattcagt tcaggcattg ggacccgaat tgcagagatg acagacacac
    18181 acccggcggc cgcgttcccc tccccccggc acgatcatgc accctgcgtt gcgctggcgc
    18241 tggcgcgggc ggagcgtgcc tttgaggaga aaggcctgcg tctcaccgac ttgaggcgcc
    18301 gcgtcctcga ggagatcgcc ggttcgcatc acgccatcgg cgcctacgat gtcctcgagc
    18361 gtctcagccg gaaagggagc cgtctcgctc cgatctccgt ttatcgcgcc atcgatgcac
    18421 tgctgagcgc cggtgtcatc catcggctcg aaagccggaa tgcattcttc gcctgccatg
    18481 cgacccatac cgcggatcgc gatcagatcg tgctggcctg cgagcgttgc gggcgggtgg
    18541 cagaggtgcc tggcaaaccg gttttcgatg ccatttccag catcgcgaaa aaggcggcgt
    18601 tcaagcccgt gcatacggtc gcggaggtga gcggcgtctg cgacgactgc caggcccgct
    18661 gagctggtgg atgagcgcat gaacatcttg ccgatcaatg cggcctccga ccttggccac
    18721 gagggcaggt ggagccacga ccatgcccat ccgcaccacc atgcgccgaa cgaggcgagc
    18781 acattggggc tagcgccgcg tgtccaggat gcgctcatca gcgcccgtgg gctgtggctt
    18841 acccgaagcg gccggacaat tctggaagat atcgatcttg acatcggcag aggggagatc
    18901 gtcaccctga tcggccccaa cggcgcaggc aagacgacgc tcgtgcgcgt gctgctgggc
    18961 ctcacctcac ccgatcgcgg cgaaatccgc cgcagccctg gcctgacgat cggctacgtg
    19021 ccgcagcgct tcgacgtcga ccgcgcgatc ccgatgacgg tggcccgttt cctggccctc
    19081 ggccgctccg gcagaccaga acgggtccgc gccgtgctgg ccgaggtcgg cgccagccgt
    19141 gtgctcgagc agcagatcgt gagcctgtcg ggcggggagc tgcagcgcgt tctcctcgcc
    19201 cgcgccctgc tgcgcgatcc taacctgctc gtgctcgacg agcccgttcg cggcgtcgac
    19261 tacgctggcg aggcggagct ctatacgctc atcggacgtc tgaggacggc gcggggcctg
    19321 agcgtcctcc tcgtctcgca cgacctgcac atcgtcatgg cccagagcga tcgcgtcgtc
    19381 tgcgtcaacc gccatatctg ctgctccggc ataccggaag ccgtcgccaa ggacccgtcc
    19441 tatgcccggc tgttcggcgc gcacgcggca cgcgcgttcg ccatctacag ccatcagcat
    19501 gatcaccacc acgatctcgc cggcgaaacg cgcccggccg cacccgacgc ggccgacgga
    19561 ccgggccggc aggaggcgtg actgcatgga cccgtttctc attcgggcat tcgttgccgg
    19621 catcggcgtg gctatcctgg cagctccgct gggctgcttc gtcgtctggc agaggatggc
    19681 ctatttcggc gaggccgtcg cgcaagcaag cctcattggc gttgctctcg ggcttgccat
    19741 ccaggccgac ttgacggcgt cggcgctcgt cgtatcggcc gtgatggctc tcctgctcat
    19801 cgtgctgtcc cggcaaagcc tggtgccact cgactccctg ctcggcctgc tcgcgcatgc
    19861 ggcgctggcc gtcggggtca tttcggcctc actcgtgcaa gggccggctg tcgacctgct
    19921 cggctacctg ttcggggaca tcttcgccgt ttccgcaggc gacctgtggt gggtgttcgg
    19981 cgggggagcc gttatcctgg cggcacttgc ctggctctgg cagccgttgc tgtccatcgc
    20041 cgtccacgag gagctggctg cagccgaagg gatcccacgg gacctggtga agggcgtctt
    20101 cgtggtcctg ctggcactcg ccatcgccat tgcgatcaag atcgtcggca tcctgttggc
    20161 aattgcattc ctcatcatgc c
//
LOCUS       DASVDU010000065         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1271027, whole genome shotgun sequence.
ACCESSION   DASVDU010000065 DASVDU010000000
VERSION     DASVDU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271027"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VF226_04480"
     CDS             <1..698
                     /locus_tag="VF226_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7073275.1"
                     /translation="PGRFSFNVKGGRCEACQGDGVIKIEMHFLPDVYVTCDVCKGKRY
                     NRETLEVTYRDKSIADVLDMTVEEGAAFFQAVPAIRDKLETLARVGLGYIKIGQQATT
                     LSGGEAQRIKLAKELSRRATGRTLSILDEPTTGLHFHDVAKLLDVLHELVDAGNTAVV
                     IEHNLEVIKTADWIIDLGPEGGDAGGRIVAVGTPEDVAANPESYTGQYLKELLGRRAK
                     TSGRNGRKREAAE"
     gene            783..1019
                     /locus_tag="VF226_04485"
     CDS             783..1019
                     /locus_tag="VF226_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073276.1"
                     /translation="MTRIERIAPFLEALSDDAFEDFLAAASHAARADTIYETLSPSEK
                     AEIDAAIARLDANEGVPYDELKARLAANLKAAGL"
     gene            1016..1303
                     /locus_tag="VF226_04490"
     CDS             1016..1303
                     /locus_tag="VF226_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7073277.1"
                     /translation="MRITFDPRAAVELEAQIGYLIERDALVAAARLKARCDDFFEKFL
                     AHHPRTGKFIAERHIWEIWIPGTRLVIWYRFTQDELQIIRIWHAAQDRGRG"
     gene            complement(1338..2280)
                     /locus_tag="VF226_04495"
     CDS             complement(join(1338..1943,1945..2280))
                     /locus_tag="VF226_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7073278.1"
                     /translation="MAKPYSEDLRSRVANAIRSGESSRSIAARFGLAPSTVIKWSKRL
                     RETGSVAPAKFGGHLRCRLEAHRDFVCRQVEEVPHLTLHRLKDLLRERGVIVSHDTVW
                     RFLRREGLSFKKTLLAAEQHRADVVRRRRRWRQAMPHLDPARLVFIDETWIKTNMAPI
                     RGWGPKGQRLKGFAPDGRWKTLTFLAALRIDALLAPCVIDGPINGALFRAYVEQLLVP
                     SLSPGDIVVIDNLGSHRSRAVRDAIVAAGARLAFLPPYSPDLNPIEQVFAKVKHWMRM
                     AQARCIAAVHDRIATLVRSIPAAECANYLRNAGYASI"
BASE COUNT          462 a          776 c          770 g          432 t
ORIGIN      
        1 agccgggccg cttctcgttc aacgtcaagg gcggccgctg cgaggcctgc cagggcgacg
       61 gcgtcatcaa gatcgagatg cacttcctgc ccgacgtcta cgtcacctgc gacgtctgca
      121 agggcaagcg ctacaatcgc gagacgctgg aggtcaccta ccgcgacaag tccatcgccg
      181 acgtgctcga catgacggtg gaggaaggcg cggcgttctt ccaggcggtg ccggcgatcc
      241 gcgacaagct cgagacgctg gcgcgcgtcg gcctcggcta catcaagatc ggccagcagg
      301 cgacgacgct ctcaggcggc gaggcgcaac gcatcaagct cgccaaggag ctctcccgcc
      361 gcgcgacggg ccgcacgctc tccatcctcg acgagccgac caccggcctc cacttccacg
      421 acgtcgccaa gctgctcgac gtgctgcacg agctggtgga cgccggcaat acggcggtgg
      481 tgatcgagca caacctggaa gtcatcaaga ccgccgactg gatcatcgat ctcggccccg
      541 aaggtggtga tgctggcggt cgcatcgttg ccgttgggac gcccgaggac gtggccgcga
      601 acccggagag ctacacgggg cagtatctaa aggagctgct gggccgtcgc gccaagacga
      661 gcggccggaa cgggaggaag cgggaggcgg cggagtaggt tctaagtctc tcgatccccg
      721 gaggcttacg cgccagatca agtcgcctta cactgcagga tcgtcgtgct atattatcgc
      781 gcatgacacg aattgagaga attgcgccgt ttctggaagc gctgtcggac gacgcgttcg
      841 aggacttcct cgctgcagca tcgcatgcgg ccagggcgga cactatctat gagacgcttt
      901 caccaagcga aaaagctgag atcgatgccg ccatagcgcg cctcgatgcc aatgagggtg
      961 tgccctacga cgagctcaag gctcgcctcg ccgccaatct caaggctgct ggcctatgag
     1021 aatcacgttc gatccgcgcg cagcggtcga actcgaagcg cagatcggtt atctcatcga
     1081 gcgtgatgct ctcgtcgcag ctgcccggct caaggcccgc tgtgatgatt ttttcgagaa
     1141 attcctcgca caccatcccc gcaccggcaa attcatcgca gagcggcata tctgggagat
     1201 ctggatccca ggcacacgcc tggttatctg gtatcgcttc acgcaggacg agcttcaaat
     1261 tatccgtatc tggcatgccg cccaggatcg tgggcgaggg tgaacgaccg gcgttggacc
     1321 tctagagccc ttcagaatta gatggaagca taaccggcat tccggaggta gttggcacac
     1381 tcggccgcgg ggatgctgcg gacgagggtt gcgatccggt cgtggacggc ggcgatgcag
     1441 cgcgcttgtg ccatccgcat ccaatgcttc accttggcga agacctgctc gatcgggttg
     1501 aggtctgggg agtagggcgg gaggaatgcg agcctcgctc ctgccgccac aatcgcatcg
     1561 cggacagcgc gcgagcggtg actgccgaga ttgtcgatga cgacgatgtc accaggcgac
     1621 aggctcggca ccaggagctg ttcgacatag gcgcggaaca gagcgccatt gatcgggccg
     1681 tcgatcacac acggcgcaag cagcgcatcg atccgcaggg cggccaggaa cgtcagcgtc
     1741 ttccagcggc cgtcgggcgc gaagcccttg aggcgctgac ctttcggccc ccagccgcgg
     1801 atcggcgcca tgttggtctt gatccacgtc tcatcgatga aaacgaggcg cgcgggatcg
     1861 agatgcggca tggcctggcg ccatcgccgg cgccgccgca ccacgtcggc ccggtgctgc
     1921 tcagccgcca gcagtgtttt ttttgaagct caatccctcc cgtctcagaa agcgccagac
     1981 cgtgtcgtgc gagacgatga cgccgcgctc tctcagcaag tccttgaggc gatgcagcgt
     2041 caggtgcggc acctcctcga cctggcgaca gacgaaatcg cggtgcgcct cgagccggca
     2101 cctcagatgg ccgccgaact tcgccggcgc cacgcttccg gtctcacgca gccgcttcga
     2161 ccacttgatg accgtcgacg gcgcaaggcc gaaccgcgcg gcgatggagc ggctgctctc
     2221 gcctgacctg attgcgtttg ccacccgcga gcgcaggtct tccgaatagg gcttcgccat
     2281 gcatgctggc cccctctctc cagcatgcat gttgaatcag aaagcgactg atttgggaat
     2341 cctctcgatt ctgtctagtt ctgaactgct ctagtgccgg ctgcgcgggt ctcgtttgtg
     2401 cacttgcgtg cattgcacgc acggctcacc gaaacacaga
//
LOCUS       DASVDU010000066         9752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1657744, whole genome shotgun sequence.
ACCESSION   DASVDU010000066 DASVDU010000000
VERSION     DASVDU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657744"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VF226_04500"
     CDS             complement(<1..1070)
                     /locus_tag="VF226_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7073279.1"
                     /translation="MRPNPFRKAVAFAAPTVSAIGWLVLTASAWAQDDDPALSLNEPL
                     SLDEPLTAEALIAWVLRENAGLDAAEAAAEAAAYRIEPAGSLDDPMLNVGTAPRSSDQ
                     NIDFSQRLPWPGTLKAREAAARYEATAAEFNVGVECLTLASAAKSAYAEWYFAARGLE
                     IHHQVQALVEELIATAETRYAAGRALQQDVLQAEVERAELETQELELLREQAAALARI
                     NGLLNRPPYAPLPPAGPISTELPALDAEALGRLALSRHPELRRLDAEISVAESRITVA
                     RKAFFPDFEIRAGYNEMWSDPDQRAMLGVSINIPFDRGKRQAELDRAQAERRRAESTL
                     SNERAQLLADVARARAEVVEAI"
     gene            complement(1180..1578)
                     /locus_tag="VF226_04505"
     CDS             complement(1180..1578)
                     /locus_tag="VF226_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073280.1"
                     /translation="MVKAVLLALLAFAGPLQAQLIYACSADEGMEGMVGSCCYDQDGN
                     LVSPDCDTDAVTDTSPCMEPVTDEVDQSARTQAPTERDQSRDTPPATGPPLAEHLPAL
                     TWQPAHRAVPAVVPHDIGTDTYLITRRLRL"
     gene            1762..1986
                     /locus_tag="VF226_04510"
     CDS             1762..1986
                     /locus_tag="VF226_04510"
                     /inference="COORDINATES: protein motif:HMM:NF016130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX7073281.1"
                     /translation="MTAALQGCHTMLIDGYVIEGHAPLGLLKKLLAERPTIEGISLPG
                     MRLGSPGMAGLKLGRFTVYAISGGPLEVLP"
     gene            2134..2415
                     /locus_tag="VF226_04515"
     CDS             2134..2415
                     /locus_tag="VF226_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073282.1"
                     /translation="MTVRTEVWKPHGASGDGLLGRMRARVGKPGLLVLAALAALIAAA
                     AFNWSSLVPVRMAPLLLALVPCALMCALSLCKGRGGSCSSDGAERAKTS"
     gene            2542..2790
                     /locus_tag="VF226_04520"
     CDS             2542..2790
                     /locus_tag="VF226_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073283.1"
                     /translation="MATTAALAALSGGAVSFAQSEGQESESQQGQTADREQMNMMTMM
                     DRMSQMMERMDRMMQMCNDMMTQMQQEQAETPPEGDAQ"
     gene            2899..3201
                     /locus_tag="VF226_04525"
     CDS             2899..3201
                     /locus_tag="VF226_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7073284.1"
                     /translation="MAAHRSGLNPKTIRYYESKGLIPSRRRQANGYRDYMEADVHTLS
                     FVKRARSLGFSVQEVAEPLSLWRNRRRSSATVKASREDISTCSTERLRSSRRCARP"
     gene            complement(4391..5518)
                     /locus_tag="VF226_04530"
     CDS             complement(4391..5518)
                     /locus_tag="VF226_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7073285.1"
                     /translation="MAEVGQSLYVSAGLFWQAFWALAFGYAFSSLIQVLVPREWIARH
                     LGRPRLKETGLAMLLGPASSACSFAALSAGRALFVKDAALVPTLAFLFGSTNLAPPVA
                     ALAWIFLGWQFALALVVGSVAHVAVMWLVVHLTYPKKMVENAKRNAQKTAEGRFGVHG
                     DPTEGLPRHWREKVQSAEARYRIGITYRGEWGMVWKDLFFGFLVAGIVATLVPDSVFQ
                     AIFPTELPPLLLVPLHALLGPALAILTIIGSMGNGPLAAVLWENGVLFSGIVAFLYAD
                     FVVIPSLRINATYYGWRFAFYLAAVFAISAVGAGVIMHALFSALSLIPATKAGRVQEL
                     AQFEIDYVFFLNIAALAVSGVLVWLGRRRHPQQQAQPQESD"
     gene            5920..6474
                     /locus_tag="VF226_04535"
     CDS             5920..6474
                     /locus_tag="VF226_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073286.1"
                     /translation="MQLRLIAATPHWSAIHLVMLAGTGLVIAGIWVRLLIARSGAQPA
                     LLAALAIVTVGEAINALNIVYMARTGSYLAAAFDAGNEAAAPLYAATHPIGLMAARFG
                     NYLVALGAFALGWAEWRGTAHPRLFAGLAWLASALGFAGVLFFAETSRFTLAAVATLS
                     VWQLAVAVWALSVARSGGGTPNRR"
     gene            6672..6968
                     /locus_tag="VF226_04540"
     CDS             6672..6968
                     /locus_tag="VF226_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7073287.1"
                     /translation="MTDGTSPVAQAKDPKQTTARDVMSSPVITTKPEDNVEDCCGEME
                     RNQIRRVPVVDESGTCCGMAAQADTAKTLSEHEAAELVRDVSQSTQEASRVACC"
     gene            8260..>9752
                     /locus_tag="VF226_04545"
     CDS             8260..>9752
                     /locus_tag="VF226_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEX7073288.1"
                     /translation="MLTRRILLAGAAAFGGAATLSPFLPAWARSGTPGLTPNIPTLSG
                     EDIALRIGHTAFAVGGRSGHAITVNGSLPAPLIRLREGQNVRIAVTNDLDEDTSIHWH
                     GILLPFQMDGVPGISFPGIRPRETFVYEFPVRQSGTYWYHSHSNLQEAVGHYGPLIFD
                     PASPDPVQYDREHVLVLSDWSFLHPHEILRKLKAEPGYFNRQRQTLAGLLSDGDPEQR
                     MTLRDRLMWGRMRMDPTDIADVTGTTYTYLINGHGPQENWTGLFQPGERVRLRIINAS
                     AMTVFHFRIPDLAMTVVNADGQNVRPVEVDEFQISVAETYDVIVEPAGDRAYTIVAES
                     VDRSGMACATLAPRLDMTAPVPPLRPRPTLGMADMGMGGMAGEAAGSSDAHMDHAATP
                     NMDRGAAAEGSRGAMAGMDHGSAGAAGHSGMSTRDPANAPADMKVGVGVDMIAPMPQD
                     RTGHPGLGLENVGHRVLTYRDLVSLAPNPDPRPPSRSLEIHLTGNMER"
BASE COUNT         1869 a         2961 c         3126 g         1796 t
ORIGIN      
        1 tcgatggctt cgacgacttc ggctcttgca cgcgcgacgt cggccaggag ctgcgcgcgc
       61 tcgttactga gcgtcgactc cgcccgacgc cgttcggctt gggcgcgatc gagttccgcc
      121 tggcgcttgc ctcgatcgaa ggggatgttg atcgagacac cgagcatcgc gcgctggtcg
      181 gggtcgctcc acatctcgtt atagccagcc cgaatctcga aatcggggaa gaacgccttt
      241 cgcgcaaccg tgatgcggct ttccgcgacg gagatctcgg catccagcct cctcagctcc
      301 ggatgccgac tgagtgcgag acgcccgagc gcttcggcat cgagcgcggg cagctcggtc
      361 gaaatggggc ccgccggcgg caacggagcg taaggaggtc tgttcaacaa tccgttgatg
      421 cgggcgagcg cggcggcctg ctcgcgtagc agctcgagct cttgcgtctc gagctcggcg
      481 cgttccacct cagcctgcaa gacatcctgc tgaagtgctc ggccagcggc gtaacgcgtc
      541 tcggccgtcg cgatcagctc ctcgaccaag gcttgaacct ggtgatggat ctcgagccct
      601 cgagcggcaa agtaccattc cgcgtaagcc gatttggccg cggacgcgag ggtgaggcac
      661 tcgacgccga cattaaactc cgcggccgtt gcctcgtatc gagccgcggc ttcgcgcgcc
      721 ttcagtgtgc cgggccacgg gagacgctgg ctgaaatcga tgttctggtc agacgaccgc
      781 ggcgccgtac cgacgttcag catcgggtca tcgagggagc cagcgggctc gattcggtat
      841 gccgcggcct cggccgcggc ttccgcggcg tcgaggcctg cgttttcgcg caggacccag
      901 gcgatcaggg cctcggccgt cagcggttcg tccaagctca gcggctcatt caagctgagc
      961 gccggatcgt cgtcctgcgc ccatgccgag gctgttagga cgagccagcc gatggctgaa
     1021 acagtaggcg ccgcaaacgc gacggccttg cgaaaagggt tgggacgcac ggtgcgacct
     1081 ccttggcgaa ttgatcggga tccggccggt gccggatgca cgacggtaga tttgccgcgc
     1141 ggaccgtccg gcgcggcagc caattcgcgg gggggcgact tagaggcgca gtcgtcgcgt
     1201 tatcagatag gtatcggtcc cgatgtcgtg aggaacgacg gcggggacag cgcgatgagc
     1261 gggttgccag gtaagagcgg gaagatgctc tgccagaggc gggccggttg ccggaggtgt
     1321 gtcccggctt tgatctcgtt cggtcggcgc ctgggtccgc gccgactggt ctacctcgtc
     1381 ggtgaccggt tccatgcacg gagacgtgtc agtgacggca tccgtgtcgc aatccggaga
     1441 gacgaggttg ccgtcctggt cgtaacagca gctgccgacc atcccttcca tgccttcgtc
     1501 ggcgctacac gcgtagatca gctgggcctg gagtgggcca gcgaaggcga gaagcgctag
     1561 gaggacggct ttgaccacga tggaatctta gttcgggggc gacagtggaa caagttctcc
     1621 gttagtgcaa tcacttgaac aagaagttcc aagtggcctt ctgccacgtg ctacggccga
     1681 gctcggcggg gcgagccttg acattccagc actggaaggc ttacgacgcc cgatcttgcg
     1741 ctgatcaagg cgaagcacgg cgtgacagcg gctctgcagg gctgccacac gatgttgatc
     1801 gatggctatg tgatcgaagg ccacgcgccg cttggcctgc tcaaaaagct gctggcggag
     1861 cgcccgacaa tcgaaggcat atcgctaccc ggcatgcgtc tcggctcgcc tgggatggcc
     1921 gggctaaagc tcgggcggtt taccgtctac gcgatttcgg gcggacccct cgaggtgttg
     1981 ccgtagaata ggcgatcgac gaccggtcgg gggcgtgtgg cccggattga gggtgagcgg
     2041 cgccgacgta tgcagctgca acttgcggac gaccgcgccg ttaaaaaaaa aaaatacggt
     2101 tgatcgggag cagaaggacg aagaggagtt ccaatgactg tccgaacgga ggtgtggaag
     2161 ccgcacggcg cgtcgggcga cggtttgctc ggccgtatgc gcgcccgagt gggcaagccg
     2221 ggcctgctcg tgctcgcagc cctggctgcc ctcatcgctg cggcggcctt caactggagt
     2281 tcgctcgtcc ccgtccggat ggcgccgctg ctgctggcgc ttgtgccgtg cgcgctgatg
     2341 tgtgcgctca gtctatgtaa gggacgcggc ggctcttgtt cgtccgacgg tgcagagaga
     2401 gccaagacca gctgaagtca gcgtcagcgc cattggtgat accgtcccca agtggcatag
     2461 gttctgcatt catgggctct gtttattgaa cgcttggatg gaagaggagg taagacgatg
     2521 cgtagcaatt ggaaaaagtt gatggcaacg actgcggccc tcgcggctct gtccggaggc
     2581 gcggtctcct tcgctcaatc cgagggacag gagtcggaga gccagcaagg ccagacggct
     2641 gatcgtgaac agatgaatat gatgacgatg atggatcgta tgagccagat gatggagaga
     2701 atggatcgca tgatgcagat gtgcaacgac atgatgacac agatgcaaca ggagcaggca
     2761 gaaacgccgc ctgaaggcga tgcgcaatag cagcgcgaga tgggtccggc cgagtgccac
     2821 tcatcggcgg acctgtctgc cgcaccaatt gccgcatcaa ttatcgagcg ccgccaggag
     2881 gcaggattga acatcgggat ggcagcgcat cgatccgggc tgaatccgaa gacgattcgc
     2941 tactacgaga gcaaaggctt gataccttcc aggcgccgtc aagcgaacgg ctatcgtgac
     3001 tatatggaag ccgacgttca tacgctcagc ttcgtaaagc gcgcgcgcag ccttgggttt
     3061 tccgttcaag aggtcgccga gccgttgagc ctgtggcgca ataggaggcg gagcagcgcg
     3121 acggtcaagg cgtcgcgcga agacatctcg acgtgctcga ccgaaagact caggagctca
     3181 aggcgatgcg cgcgacccta ggggaggttg gtcgaacgct gccagggcga cgatcgctcc
     3241 agctgtccta tcttagagca gcttgaagat gccgatcgcg cagtcgaagg cgttcggcta
     3301 tgcgcttgtg catacaggga aaacgcatca cttggcgtaa aagcaagacc gagctcgacc
     3361 gtcttgtcct cattcgcatc cttccgttgg tcccagtcgt tgttaacgcc cagcgtcaag
     3421 ctgagcgcat cactgactcc gaacgtagct cgtaccctcg tatgggagaa agggatcgca
     3481 taaccgaaga ggatggagcg cgagtatgta ggattgccgg tcgagtcgat gacctcggcg
     3541 ccggcgagcg ttacatgctt gccggcaatg aacgtcgccc gccgcctgcg taccgaacgt
     3601 acgcctgagt cacatcgaag tcattcgtcg atgtgtcgaa ggacttgatg gtgcgtgcat
     3661 ctcggccagc cgtgatattc acggagccgc cccaaccctc ctcgtcgggt tggatcgaag
     3721 cgaagttcgc cgcttgatgg tagttaaagg aatcgggttc cgtgtcgaag acacgccgcc
     3781 cgtaaacacg ccggattccg acagccagga ataggcagcg tcgacgtagc ctcggagttc
     3841 aatgccgcag ctttccagca catcgtccaa gctcgcagta cctctagcga gcacaaggtc
     3901 atgagggcca cgagtacgcc gcgttctcag gcgcgggtct gctcgacaat tatgcaggcg
     3961 gcgcgccaaa acggagcgca cgcttgcggt gatatcgact acttgtctgc aacatcttga
     4021 accgcgcttg tcgtagcacg gttatcgcga acggctggcg caaaccccac attcggagga
     4081 gggccgcgca atgacaagag caatactacg tcccgctcca cggttcttgg caccgttagg
     4141 ctgcgaaggt ctggacgtag gttgccatgc ttgaactcat cggcgtgcgc ttgccttgca
     4201 cctcgccgtt ctctcgcgca tgtgctctgc gctcgccgcg ctacggtgcg agcttgtccg
     4261 ggacgtgtcg cacccaacga aggaagcctc acgcgtcggg tgttgttaac gacttgcttg
     4321 tgcgacgtac gaggacgccc gaggcaaggc atcgccggct agaagggaat gagttcggtt
     4381 gcggagcgag tcagtcgctt tcctgcggct gagcctgctg ctgagggtgg cggcgccggc
     4441 caagccagac gagcacgccg gaaacggcca gtgccgcgat gtttaggaag aacacatagt
     4501 cgatctcgaa ctgtgccagt tcctgaacac gcccggcctt cgtcgcgggg atcaagctta
     4561 gtgcggagaa cagcgcatgc atgatgactc cggcgccaac tgccgagatg gcgaagaccg
     4621 cagcaaggta gaacgcgaag cgccacccgt agtaagtcgc gttgatcctc agggatggga
     4681 tcacgacaaa gtcggcgtaa aggaacgcga cgatgccgga gaagaggact ccgttttccc
     4741 aaagtaccgc ggcgagtgga ccgttgccca tcgagccgat gatcgtgagg attgcgagcg
     4801 cgggtcccag caatgcgtgg agcggtacga gcaggagcgg cggcaactcc gtggggaaga
     4861 ttgcctggaa cacagagtct gggacgagcg tggcgacgat gccggcgacg aggaagccga
     4921 agaagaggtc tttccagacc atgccccact caccgcggta ggtgatccct atgcgatatc
     4981 gtgcctctgc gctctgaacc ttctcgcgcc agtgccgcgg tagcccctca gtaggatcac
     5041 cgtgcacgcc gaagcgaccc tcggcggtct tctgtgcatt tctcttcgcg ttctccacca
     5101 tcttctttgg gtacgttagg tgaacgacca gccacatcac cgcaacgtgg gcaaccgagc
     5161 cgaccacgag cgcgagcgcg aactgccacc ccaagaagat ccaagccaag gcagcgaccg
     5221 gaggcgcgag gttggtggag ccgaagagga acgctagcgt ggggacgaga gccgcatcct
     5281 ttacgaacag agcgcgtccc gcggacaacg cggcgaacga gcaggctgag gatgccggtc
     5341 ccagcagcat cgccagcccc gtctccttca ggcgtgggcg cccaaggtgt ctggcgatcc
     5401 actcgcgggg gacgagaacc tggatcagcg acgaaaacgc gtagccaaag gcaagcgccc
     5461 agaaggcctg ccagaagaga ccggcgctga cgtatagcga ctggccgact tcggccacgt
     5521 tatagtgccg tcctgtgcca gttactgcaa cagcgtgcaa cccttcgatc catcgcactg
     5581 aattcctttc gtactctgcg tcgaagccga cgctaagcaa gaggtgtacc aagatcgacc
     5641 tgattgccct ggtcagcacg aagggaccat ggcccgcctt tgacactaac gtatcgatgc
     5701 ggtaggtgta gttacgggtc ttgtcctttt tcgacaaaga agggaccggc ctgcgcatcg
     5761 tcacggtgac cggcgacgtc cgcgctggca atgggtcagt ccactcgcat gtaaaacgtg
     5821 atccctctcg tccccctgct tgccctcgcg ctcctgttcg gcacagccct ccttggcgtt
     5881 ggcgcgctga tgcaccccgt gcttgcagga gatgcgtcaa tgcagctgag gctgattgcg
     5941 gccacaccgc actggagtgc aattcacctc gtcatgctcg ccgggactgg gttggtcatt
     6001 gccggcatct gggtgagact tttgatcgcc cgctccggcg cgcagccagc gctactcgcg
     6061 gcgctcgcca tcgttacggt cggcgaggcc atcaacgcgc tcaatatcgt gtacatggcc
     6121 cgtaccggct cgtaccttgc ggcggcgttc gatgcaggca acgaagcggc cgcgcctctt
     6181 tacgctgcaa cgcatccgat cgggctgatg gctgcacgct tcggcaacta cttggtcgcg
     6241 cttggagcat tcgcgctcgg ttgggcggag tggcgcggca ccgcgcaccc gcggctcttt
     6301 gccggcctag catggctcgc ctcggccttg ggctttgccg gcgtcctatt cttcgccgag
     6361 acgagtcgct tcacactcgc cgcagtcgcg acgctctcag tctggcaact tgccgtagcg
     6421 gtctgggcgc tgagcgtggc gcgcagcggt ggcggtacac cgaaccgtcg ctgagcaggc
     6481 tgcctgcggt cgtctcaatt cacgggcgaa gatcaaaagg aggaatcgat atgcaagtgc
     6541 gagagatcat gacggaaaac ccggcgagct gcgcgccgga agccaacctt gaggacgtgg
     6601 ccaagtgatg gtggatcacg actgcgggga gcttcccgtc ctttgaccaa caaagccggc
     6661 ctatcggagt cgtgacagat gggacatcgc ctgtcgcgca ggccaaggat ccaaaacaga
     6721 caactgcgcg cgacgtaatg tcgagcccgg tcattaccac gaaaccagag gacaacgtcg
     6781 aggactgctg cggcgagatg gagcgaaacc agattcgccg cgtaccggtg gtcgacgaga
     6841 gcggcacgtg ctgtgggatg gccgcccagg cggacactgc caagacgctg tctgagcacg
     6901 aagccgccga gctcgtccga gacgtgtcgc aatcaacgca ggaagcctca cgcgttgcgt
     6961 gctgttaaag acattcgtgc tcggggtgcg acgaacgtcg gaatcggccg aagattccgg
     7021 accaggatta tggcttgaag ggcttggacg tccattcgga gacgcggcgg cagcaacgtg
     7081 gcggagccag acggccgcga gacaaagggc gacgggagtc gaccactctc atgccggcta
     7141 aaggcattcc caaccgctcc aagttcagcg tcttccgggg acctcgttgt ccgaagcgaa
     7201 gggcctgatg gacggtctcg tccagcgtcg aacagcggcg tttgagccag ggctaacgag
     7261 cgaggagtac cgggagatac tcgaggcggc ggaagggcag ggccatcacg ctggtaaaaa
     7321 gccggcggcc acaacgagga tctcggctct cgagccgccg gcagacagag gcgtctgtag
     7381 aatctgctgc ccctcttgac attccaatcg ctgtaacctt tatgattcgt tcacaacctc
     7441 ccctacggcg gttgatgggt atagtgttca gggtacgggt cggtatggaa ccaggcgctg
     7501 ttctcggttg cccaaaggat agacacaagg cgccacagtt cctcgcgacg tacggaaggc
     7561 ttggtcgggt gcatagatcg tgaccgtgtt agagcacatc cgaaggcgca gtaaggcggt
     7621 ggcgcgcgct attttgccgc cgcttgcttt cgtttggctt agtgcttccg catctccgtg
     7681 cgctggcatg gtgaccgact ccgtatcccg cggcgacgtc gcaccagccg tccataacca
     7741 cgacgagact tcccctctgc acgaccatgg cgcttcgagc agtggccatc aaacgagcca
     7801 tgagcatcaa agctgcccgc attgcccccc cgcgacggct tcggaccaca tcgctccgtc
     7861 cggatcgcac attccatgca acgtgttgga caaggtctcc gacagcagcc ggaccgctgg
     7921 agcgccagtc aaagtcgacc tgaagcacgt gctcccggtg gcggccgatc tgctttcgcc
     7981 tgcaactcta gtctctttcg gatcctatct ctcacccgcc agtcagcaga ttgcgcctca
     8041 gtccctcctt ccgctcaacg tccgctattg cgtgtttctg aattgagcgt cgtcctttga
     8101 cgcgcagagc cgccgccgag cggttgctgc cgtcccgacc ttctccatag ctttcgaatt
     8161 gcacgaatat gtttgcgagt cgcgaagcat tggcttcggg ctcgcggttc ccaaggcgcc
     8221 cgctcgcgcc ttgggggcaa gccgctaagg aagcaggtta tgcttactcg tcgtatcctt
     8281 cttgccggcg ctgccgcctt cgggggagca gcgacattga gtccgttctt gcctgcatgg
     8341 gctcggtccg gaacaccggg cctgacgccg aacatcccga ccctttccgg cgaggacatt
     8401 gcgctccgga tcggccatac cgcttttgcc gtcggcggcc gaagcggcca cgccatcacg
     8461 gtcaacggct cgcttccggc gccgctgatc cgtttgcgag agggtcagaa tgtacggatc
     8521 gcggtaacca acgatctcga tgaggacacc tcgatccact ggcacggcat cctccttccg
     8581 ttccagatgg acggcgtgcc cgggatcagc ttccccggca tccgtccgcg ggaaaccttt
     8641 gtctacgaat ttccggttcg ccagtccggc acgtactggt atcacagcca ttcgaatttg
     8701 caggaggcag tcggccatta cggtccgctg atcttcgacc ccgcttcgcc cgatcccgtg
     8761 caatacgatc gcgagcacgt tctcgtactc agcgactgga gcttcctcca tccgcacgag
     8821 atcctgcgca aattgaaggc ggagcccggc tacttcaatc gccaacggca gacgcttgcg
     8881 ggcttgctca gcgatggcga tccggagcag cgcatgacgc ttcgggaccg gctgatgtgg
     8941 ggccggatgc gcatggatcc caccgatatc gccgatgtga ccggaacgac ctacacatat
     9001 ctgatcaatg gtcacgggcc gcaggaaaat tggaccggtc tgttccagcc cggggagcgc
     9061 gtgcgcctac ggatcatcaa tgcctcggcc atgactgttt tccacttccg catcccggac
     9121 ctcgccatga ccgtcgtcaa cgcggacggc cagaatgtac gccccgtcga ggtcgatgag
     9181 tttcagatct cggtcgcgga aacgtacgac gtgatcgttg agccggccgg agaccgcgcc
     9241 tacacgatcg tggcggaatc ggtcgaccgc tccggcatgg cttgcgcgac gctcgcgccg
     9301 cgcctcgata tgacggctcc cgtgccgccc ttgcggcccc gcccgacact cggcatggcc
     9361 gacatgggca tgggcgggat ggcgggcgaa gcggccggtt cgagtgatgc gcatatggat
     9421 cacgctgcga cgccgaacat ggaccgcggt gcagccgccg aagggagccg tggcgccatg
     9481 gccgggatgg atcacggctc cgcgggagca gccggacata gcggcatgag cacgcgcgat
     9541 cctgctaacg cgcctgcgga catgaaggtc ggcgtgggcg tagacatgat cgctccgatg
     9601 ccgcaggacc gcaccggcca tccggggctc ggtctcgaga acgtcggaca cagggtgttg
     9661 acgtatcgcg atctggtttc gctcgcccct aatccggatc cgcgcccacc gtcgcgatcg
     9721 ctcgaaattc acctcactgg caacatggag cg
//
LOCUS       DASVDU010000067         7536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_522000, whole genome shotgun sequence.
ACCESSION   DASVDU010000067 DASVDU010000000
VERSION     DASVDU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522000"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VF226_04550"
     CDS             <1..918
                     /locus_tag="VF226_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073289.1"
                     /translation="GVHALTGAYQSGITLTASAIAERYGIPFVNGESVAANLTERGFK
                     WFFRTTPIAADIARTYMRFLKDLKEKGQKIDLIAVVNENTEYGTSIATVLRETAKEFG
                     FNIGLEIPYSANTTDVQSQVLQLKDRKPDVVIFVSYTSDAILYMKTMKAMDYKPPVLI
                     GDDSGFSDGAFIKTVGDIAQGAINRSAWDIGKPGSTTYLINEMYKEIRGADLDDTAGR
                     AMQGLFVLADAINRAGSTEPAKIQAALRATDLKPNQLMMGYRGVKFDEKGNNILADAL
                     LIQLQGDRYVAVWPSEAATAPLMLPYKGW"
     gene            1020..1883
                     /locus_tag="VF226_04555"
     CDS             1020..1883
                     /locus_tag="VF226_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073290.1"
                     /translation="MSMSLLQVVVSGLLLGALYALFASGLTLIWGMMNVVNFAHGDFV
                     MLAMYTAFVLWQTLGIGPAGSVPIAALLMATIGIIVYFSLVRHVMHGPMIAQILGTFG
                     LALFLRYAVFWYFGSNFLSLPDNLVGGTFDFGGIRVPSSRLLAGVVALLVTAALHLLL
                     TRTALGSRMLAVAEDQAAAQLMGIQPHRMQAVAWGLAAAATGVAGALFATFFYITPTV
                     GETLVIVAFVTVCLGGFGSVPGALVAGLLIGVIESLSAYWIGAVYKDIVVYSLFVAVL
                     WLRPQGLMGKA"
     gene            1883..2935
                     /locus_tag="VF226_04560"
     CDS             1883..2935
                     /locus_tag="VF226_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073291.1"
                     /translation="MSQVSRGLERSPVLGKKLAGQSSVSLKRLLWPAIWFGIIAVYPL
                     VWQTPFQQRVGALVLLYAIAASGWNILGGYAGQMSIGHVVFFGCGAYGALAVYHHLGL
                     PPIAGLPLGVLASILIAALIGVPTLRLSGHYFSMATIGVAELVRLIVINSEFLGAAVG
                     LSGPAVPRTIMDLSFISALPYYYLFLAVLICTLALTWRMENSRMGYYLRAIKDSERAA
                     RALGVAAGRYKLWAFMFSAAVTAVAGSLYAIMFGFVDPDSGLGILISVKMLIMAALGG
                     AGYLFGPLVGAVILVPLEEISNSMFGGAGRGLTFVVYGTIIMLLARFQPGGLLDLLQR
                     LPRRSRKATPGVRGDA"
     gene            2928..3677
                     /locus_tag="VF226_04565"
     CDS             2928..3677
                     /locus_tag="VF226_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073292.1"
                     /translation="MLEARGITMTFDRLKAIDDATIEVREGEILGLIGPNGAGKSTLF
                     NCLTGDLKPTAGTVHLRGQDVTPLPPEARAALGIARSFQVPLTFEGMSVLDNVMIGAF
                     LRTASTSEARERARAVIERVGLAPLADASARSLGTPGRKRLEIARALATEPSVLLLDE
                     AMAGLTPTEVRQAIELIRRIHASGVTLVIVEHIMEVIVTLAERVVVFHQGRVIANGSP
                     AAVMREPQVIEAYLGKRGARVAQRSPGRVAT"
     gene            3674..4390
                     /locus_tag="VF226_04570"
     CDS             3674..4390
                     /locus_tag="VF226_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073293.1"
                     /translation="MTAPLLSVRNLEVRYGDLIGVADVSLDVPQGGVVALLGSNGAGK
                     TTTLNAIAGLIPASAGSIIFDGEEIAGRPAYAIVRKGLALSPEGWRLFTQQTVEGNLH
                     LGATPLADRSLLPELLERVYTLFPRLAERRRQRVSTMSGGERQMLAVGRALMSNPKLL
                     MLDEPSLGLAPAVIETMYETFSRLHSQGMTILLAEQSVELALEFADHAVVLQVGRSVL
                     SGSAAELAENPEVQRVYMGI"
     gene            4469..5257
                     /locus_tag="VF226_04575"
     CDS             4469..5257
                     /locus_tag="VF226_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7073294.1"
                     /translation="MKTTEQVGRLVEVNGWQIDIIVQGYPGKSVCHGALGWSTIALLR
                     GHGNVVLIDVGSFAQRQLLLDRLAAHGLTPSDVTHVLLTHSHWDHSVNWVMFPGAKVL
                     IDGGELEWALQEPWGITPVPELYVRELSTSPQISRIGPGARILPGISAYSAPGHTPGH
                     LVYLLEGESHDVLFTGDAAKNRAEILSRTADMTYDQELSARSIESIWALWKRKPGTIL
                     VPGHDVPMVLEEGEPTYLGPRRAGISSWFGTDLEQTTLFEVTVA"
     gene            complement(5358..>7536)
                     /locus_tag="VF226_04580"
     CDS             complement(5358..>7536)
                     /locus_tag="VF226_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7073295.1"
                     /translation="ALVVIVVVLFLQTWRASIIPLAAVPVSIVGTFAVMYALGFSVNA
                     LTLFGLVLAIGIVVDDAIVVVENVERNIEEGLPPRDATYKAMREVSGPIIAIALVLVA
                     VFVPLAFITGLTGQFYRQFALTIAISTVISAINSLTLSPALSALLLKGHDAPKDKLTR
                     FLDFTLGWLFRGFNALFRRGTESYGNGVRGVLSRKALGFGVYLALVIAAFGLFRIVPP
                     GFVPSQDKQYLIGFAQLPDGATLDRTEEVIRRMSDIAMSQPGVESAVAFPGLSIAGFS
                     NSASAGIVFATLKPFSERRSPELSGPAIAMALNRQYASIKDAFIAMFPPPPVAGLGTV
                     GGFKLQIEDRVGLGYDALDAATKAFMAKAAQSPEITGLFTSFQVNVPQLYAEIDRTRA
                     RQLGVAVTDIFETMQIYLGSLYVNDFNKFGRTYSVRVQADAPFRARAEDIGQLKVRSR
                     SGEMIPLSVLLKVQQAAGPERALRYNGFLSADVTGAPAPGYSSGQAQAAVERIAAETL
                     PKGIAFEWTELTYQEILAGNSAILVFPLSVLLVFLVLAALYESLVLPLAIIMIVPMGL
                     LAAMAGVFLAGGDNNIFTQIGLVVLVGLSAKNAILIVEFARELEFSGRTPREAAIEAS
                     RLRLRPILMTSFAFIMGVVPLVISSGAGAEMRQAMGIAVFSGMIGVTIFGIFLTPLFY
                     VMLRALSGNRPLNGGDVGAQQSMETPEILPAAAPPAQTVHEDA"
BASE COUNT         1343 a         2362 c         2439 g         1392 t
ORIGIN      
        1 ggggttcatg ccctcaccgg agcttaccag tcgggcatca cgctgactgc cagtgccatc
       61 gccgagcgat acggcatccc cttcgtcaat ggcgagtcgg tagcggccaa cctcaccgag
      121 cgcggcttca agtggttctt ccgtaccact cccatcgcag ccgatatcgc caggacctac
      181 atgaggtttc tgaaggattt gaaggaaaaa ggccagaaga tcgacttgat cgccgttgtc
      241 aacgagaaca ccgagtacgg cacctcgatc gcaacggtct tgcgcgagac ggctaaggaa
      301 ttcggcttca acattggcct ggaaatcccc tatagcgcca acacaaccga cgtgcagtcg
      361 caggtgctgc aattgaagga tagaaagccg gatgtcgtca ttttcgtcag ctatacgtcc
      421 gacgctatcc tctacatgaa aacaatgaag gcgatggact acaagccgcc ggtgctgatc
      481 ggtgacgact ccggcttctc cgacggcgcc ttcatcaaga ccgtcggcga cattgcccaa
      541 ggggcgatca atcgctcagc ctgggatatc ggcaaaccgg gcagtacgac ctacctcatc
      601 aacgagatgt acaaggaaat tcgcggcgcc gacctcgatg atacggccgg tcgggccatg
      661 caggggttat tcgttctggc cgatgcgatc aatcgcgccg ggtccaccga gcccgccaag
      721 atccaggcgg ccttgagggc gaccgacctc aagccgaacc agctgatgat gggctaccgc
      781 ggcgtcaagt tcgacgagaa gggcaataac atcctggccg atgcgctttt gatccagctc
      841 cagggcgacc gctatgtcgc tgtctggcca agcgaggcag ctacggcgcc gctgatgctg
      901 ccgtacaaag gttggtgagg ccgccgtttg ccgcgctcca gaacatgtcg acttcggctc
      961 agcaggcgct ccgctgagga gtgtcctgcg ccggcccagg ttttcggatc ccctctcgta
     1021 tgagtatgtc gctcctgcag gtggtcgtca gtggtctcct cctgggcgcg ctctatgcgc
     1081 tgttcgcctc gggcctgacg ttgatctggg gcatgatgaa cgtggtcaac ttcgcccacg
     1141 gcgacttcgt catgctggcg atgtacacgg ctttcgtgct gtggcagacg ctgggcatcg
     1201 gacccgcggg ctcggtcccg attgcggcgc tgctgatggc caccatcggc atcatcgtct
     1261 acttctcgct ggtgcgtcac gtgatgcacg gcccgatgat cgcgcagatc ctgggcacct
     1321 ttggattggc tttgttcctg agatacgcgg tattttggta tttcggcagc aatttccttt
     1381 cgttgccgga caacctcgtc ggcggcacgt ttgacttcgg cggcatccgg gtgccgtcgt
     1441 ctcgactgct cgccggcgta gtcgccctgc ttgtgactgc ggccctgcac ctgctgctga
     1501 cccggactgc actcgggagc cgcatgctgg cggttgccga ggatcaggca gcggcgcagc
     1561 tgatgggtat ccagccgcac cggatgcagg ccgtcgcgtg ggggctcgcg gctgccgcaa
     1621 ccggcgtcgc cggcgcgctg ttcgccacgt tcttctacat cacgccgacg gtgggcgaga
     1681 cgctggtgat cgtcgccttc gtcaccgtct gcctgggagg gttcggcagc gtgccgggtg
     1741 cgctggtggc agggctgctg atcggggtca tcgagtcact ctctgcctac tggatcggcg
     1801 cggtttacaa agacatcgtc gtctattcgc tgttcgtcgc ggtgttgtgg ctgcggccac
     1861 agggcctgat gggaaaggcc tgatgagcca agtgtcacga ggcctggagc gctcacctgt
     1921 tctgggcaag aagctcgctg ggcagagttc agtttcgcta aaaaggctgc tctggcccgc
     1981 catctggttc ggcatcatcg ccgtctatcc gctggtctgg caaaccccgt tccagcaaag
     2041 ggtgggcgct ctggtgctgc tctacgcgat cgctgcttcc ggatggaaca tcctgggcgg
     2101 ctatgccggc cagatgtcga tcggccatgt cgtctttttc ggctgcggtg cctatggcgc
     2161 gctcgctgtc taccaccact tgggcctgcc gccgatcgca ggactgccgc tgggcgtttt
     2221 ggccagcatc ttgatcgccg cgctcatcgg tgtcccgaca ttgcggctgt ccgggcacta
     2281 cttcagcatg gccaccattg gcgtggcgga actggtgcgc ctgatcgtga tcaattccga
     2341 gttcctcggt gcggctgtcg gcctgagtgg gcccgcggtg ccgaggacca tcatggacct
     2401 gtcgttcatc tcggcgctgc cctactacta cctgttcctg gctgtcttga tctgcactct
     2461 ggcactgaca tggcgcatgg agaacagccg aatgggctac tatctgcgcg cgatcaagga
     2521 cagcgaacgc gccgcccgcg cccttggcgt tgcggctggg cgctacaagc tgtgggcctt
     2581 catgttcagt gccgcggtga ccgccgtggc tggatcgctc tatgccatca tgttcggctt
     2641 cgtcgatccg gattcaggtc tcggaatcct gatctcggtg aagatgttga tcatggcggc
     2701 gctgggcggc gcgggatacc tgttcgggcc gctggtcggc gcagtcatac tcgtgccgct
     2761 ggaggaaatt tccaacagca tgttcggcgg cgccggcagg ggcctcacct tcgtcgtcta
     2821 cgggacgatc atcatgctgc tggcacgctt ccagcccggc gggctgctcg acctgctgca
     2881 acgcctgcca cgacgctccc gcaaagcaac cccgggagtg cgcggcgatg cttgaggcgc
     2941 gcgggatcac gatgacgttc gatcggctca aggcgatcga tgatgccacg atcgaggtcc
     3001 gcgagggcga gatcctgggt ctgatcggcc cgaacggtgc aggcaagtcg actttgttca
     3061 actgcctgac cggagatctg aagcctactg ccggtaccgt tcatctgcgc ggtcaggacg
     3121 tgacccccct gccgccggag gcacgtgcag cgctgggtat cgcgcgcagc ttccaggtgc
     3181 cgctgacgtt cgaaggcatg agcgtgctgg acaacgtgat gatcggcgcc tttcttcgca
     3241 cggccagcac ctcggaagca cgcgaacggg cgcgggccgt gatcgagcgg gtcgggcttg
     3301 cgcccctcgc cgacgcctcg gcgcgcagtc tcggcacgcc cggccggaag cggctcgaaa
     3361 ttgcccgtgc gctggccacc gagcccagtg tgctgctgct cgacgaggca atggcgggtc
     3421 tgactccgac ggaggtgcgc caggccatcg agctcatccg ccgcatccac gccagcggcg
     3481 tcacgctggt gatcgttgag cacatcatgg aggtgattgt cacgctggct gagcgggtgg
     3541 tcgtttttca ccaggggcgt gtgatcgcca atgggtcccc ggccgccgtc atgcgcgagc
     3601 cgcaggtcat cgaagcctat ctcggtaagc gtggcgccag ggtcgcgcaa cgctcacccg
     3661 gccgggtcgc gacatgaccg cgccgttgct gagcgtacgc aatctcgagg ttcgctacgg
     3721 cgacctgatc ggcgtcgccg acgtctcgct cgatgttccg cagggcggcg tggtggcgtt
     3781 gcttggatcg aacggcgctg gcaagaccac caccctcaat gccattgccg ggctcatccc
     3841 tgcttcggcc ggcagtatca tcttcgatgg tgaagagatc gccggacgcc cagcctacgc
     3901 gatcgtgcgg aagggactgg cgctgtcgcc ggaaggctgg cggctgttca cccagcagac
     3961 ggtcgaaggc aacctgcacc tcggcgccac gccactcgcc gatcgaagcc ttctgccgga
     4021 gttactcgag cgcgtctata cgctgtttcc gaggctcgct gagcgccgtc ggcagcgtgt
     4081 cagcacgatg tcgggcggcg aacgccagat gctcgctgtc ggacgcgccc tgatgagcaa
     4141 cccgaagctg ctgatgctgg acgagccgag cctcggcctc gcaccggccg tgatcgagac
     4201 gatgtacgag accttcagcc gtctgcacag ccaaggaatg acgatcctgc tggccgagca
     4261 gtcagtggag ctggcgctgg aatttgcgga ccatgcagtc gtgctgcagg tcggacgcag
     4321 cgtactttct ggctcggcgg ccgaattggc ggagaatccg gaagtgcagc gcgtctacat
     4381 gggcatctga aagtgccgcg ccccggcgct tacgcttggg cggcctagct ccgctgggtg
     4441 cgacgggagc cgagacgagg gaagtgagat gaaaacgacc gagcaggttg gccggctggt
     4501 cgaggtcaat ggctggcaga tcgatatcat cgtccagggc tatccgggaa aatcggtctg
     4561 ccacggcgcg ctcggctgga gcacgattgc actgctgcgc gggcacggca acgtggtgct
     4621 gatcgacgtc ggttcatttg cgcagcgtca gcttttgctc gaccgcttgg cggcgcatgg
     4681 gctcacacca agcgacgtaa cgcacgtgct gctgacacat tcccactggg accactcggt
     4741 gaattgggtg atgttccccg gggccaaggt gctcatcgat ggcggcgagc tcgaatgggc
     4801 tctccaggaa ccctggggaa ttacgccagt accggaactg tacgtgcgcg agctctcgac
     4861 gtctccgcag atcagccgca tcggtccggg cgcgcggata ctgccgggga tctcggcgta
     4921 cagcgcgccc ggccacaccc ccggtcatct ggtctatctg ctcgaaggag agagccacga
     4981 cgttttgttc accggtgatg ccgcaaaaaa ccgggcggag atcctgtccc gcacggccga
     5041 tatgacctac gaccaggagc tcagcgcccg atccatcgaa agcatctggg cgctgtggaa
     5101 acgtaagccc ggcacgattc tggtcccagg gcacgacgtg ccgatggtcc tcgaggaggg
     5161 cgagcccacc tacctcggac cccgccgcgc cggcatctcg tcctggtttg gaacggacct
     5221 cgaacagacg acgctgttcg aggtcacggt tgcgtaagtg gaaggctttt gacgctccgt
     5281 taacagccaa ggttccaaac tggacttcag accaaatcgt ttgcgtctag ccgcgaacaa
     5341 atcggccggg acggagatca agcgtcctca tgcacggtct gagccggagg tgcagccgca
     5401 ggcaatattt ccggtgtttc cattgactgc tgtgctccga cgtcaccgcc attgagcggc
     5461 cgattgcccg acagggcacg cagcatcacg tagaacagcg gcgtgagaaa gatgccgaag
     5521 atcgtcacgc cgatcattcc cgagaacacc gcgatgccca tggcctgccg catctcggcg
     5581 cccgcacccg acgagatgac cagcggcacc acgcccataa tgaaggcgaa cgaggtcatc
     5641 aggatcggcc tgagccgtag gcggctcgcc tcgatggcgg cctcgcgcgg cgtacgcccc
     5701 gagaactcca gctcgcgcgc gaattccacg atcaggatcg cgttcttcgc cgacagtccg
     5761 accagaacga caaggccgat ttgcgtgaag atgttgttgt cgccgcccgc caggaacacg
     5821 ccggccatag ctgccagaag ccccatcggc acgatcatga tgatcgcaag cggcagcacg
     5881 aggctttcat agagcgccgc gaggacgagg aagaccagaa gcactgacag cgggaacacc
     5941 aggatagcgg agttcccggc caggatctcc tggtacgtca actcggtcca ttcgaacgca
     6001 atgcccttgg gcagcgtctc tgccgcgatg cgctcgaccg ctgcctgcgc ctgacccgac
     6061 gaatagccgg gcgccggtgc gccggtgacg tccgcggaga ggaagccgtt gtagcgcaag
     6121 gcgcgttccg gtccggctgc ctgctgcacc ttcagaagca ccgacagcgg gatcatttcc
     6181 ccggacctgg agcgcacctt cagctgtccg atgtcctcgg cccgggcgcg gaacggcgcg
     6241 tccgcttgca cgcgcacaga gtaggtgcgc ccgaacttgt tgaagtcgtt gacgtagagc
     6301 gagccgaggt agatctgcat cgtctcgaag atgtccgtca cggcgacgcc aagctggcgg
     6361 gcgcgggtgc ggtcgatctc ggcgtagagc tgcggcacgt tcacctggaa cgaggtgaag
     6421 agcccggtga tctccggcga ttgagcggcc tttgccatga aggcctttgt ggcggcatcg
     6481 agggcgtcat agccgaggcc gacgcgatcc tcgatctgca gcttgaagcc gcccaccgtg
     6541 cccagtcctg ccaccggcgg cggcgggaac atcgcgatga aggcgtcctt gatgctcgcg
     6601 tattggcgat tgagcgccat ggcgatggcc ggaccgctca gctccggcga cctgcgctcg
     6661 ctgaacggct tcagggttgc gaaaacgatg ccggcgctgg cgctgttgga aaagccggcg
     6721 atcgaaagac ccggaaaagc gaccgcgctc tcgacgcccg gctgactcat ggcgatgtcg
     6781 ctcatccgcc ggatcacctc ttcggtccgg tcgagcgttg cgccgtcggg gagctgtgcg
     6841 aagccgatga ggtactgctt gtcctggctc ggaacgaagc ccggagggac gatcctgaag
     6901 aggccgaacg ccgcgatgac cagcgccaga tagacgccaa atccgagcgc cttgcgcgag
     6961 agaacgcctc tgacgccgtt gccataggat tccgtacccc ggcggaacag ggcattgaag
     7021 ccgcggaaca gccatccgag cgtaaagtcg agaaagcgag tcagcttatc cttcggcgcg
     7081 tcgtggccct tcagcagcag cgccgacagc gccggcgaca gtgtcagcga gttgatcgcc
     7141 gaaatgaccg tcgagatggc gatagtcagc gcgaactggc gatagaactg gccggtcagt
     7201 cccgtgatga aggcgagcgg tacgaagacc gcgacgagga ccagtgcaat ggcaatgatc
     7261 ggacccgaga cttcgcgcat cgccttgtag gtcgcgtcac gcggcggcag cccttcctcg
     7321 atgttccttt cgacgttctc gaccaccacg atggcgtcgt cgacgacgat gccgatcgcc
     7381 agcacgaggc cgaacagcgt gagcgcgttg accgagaagc cgagcgcgta catcaccgcg
     7441 aacgtgccca cgatcgacac cggcaccgca gccagcggga taatggacgc gcgccacgtc
     7501 tgcaggaaca ggacgacgac gatgacgacc agcgcc
//
LOCUS       DASVDU010000068         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1060593, whole genome shotgun sequence.
ACCESSION   DASVDU010000068 DASVDU010000000
VERSION     DASVDU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060593"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..2922)
                     /locus_tag="VF226_04585"
     CDS             complement(4..2922)
                     /locus_tag="VF226_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEX7073296.1"
                     /translation="MSTDIVLITVLVALPFLGSLVAAFFPTNARNAEAWLAAGVAFTG
                     LAIALYLAGPIANGSVVRAEFEWLPQLDLNFILRLDGFAWSFVVLVCAIGLLVILYAR
                     YYMSPADPVPRFFSFLLGFMGAMLGIVLSGNLLQIVFFWELTSLYSFLLIAYWHHNQN
                     ARDGARITLTVTATGGLCLLGGMLLLGHIAGSYDLDVVLRAGDGITTHQLYLPTLLLI
                     AVGVLTKSAQFPFHIWLPRAMAAPTPVSAYLHSAAMVKAGVFLLARLWPVMAANDLWF
                     WIFTPLGVTTLLMGAFLALFQQDLKGLLAYSTISHLGLITLLLGLGSPLAAVAAVFHI
                     MNHATFKASLFMAAGIIDHEAGTRDVRRLGGLYRAMPFTAALAMVAAAAMAGVPLLNG
                     FLSKEMFFAESIEQHVVSTLDDSLPYVATLAGMFSVAYSLRLIHQVFFGPPATDLPRT
                     PHDPVLWMLLPVAFLVLTCILVGVAPEATVSGFLSIAVRGILGSATPEYSLAVWHGFT
                     PALWMSLIALVGGVAVYTALQAYLAGDIEGAPILRRLKGQRIFERAVALLSWRGAKGL
                     VQSLSSNRLQPQIALLLSVVVLAAVWPLHKASISLYLPKLSEVDVSFLLVWVVGCFCA
                     LGAAWQAKFHRLAAVILMGGAGLSTSITFAWGAAPDLALTQLLVEIVTTVLLLLGLRW
                     LPKRQPEADRPADLTARMRRLRDLMIATAGGLGIGALAYAVMTRPLTYSISSNFLERA
                     YTEAGGRNVVNVILVDFRGFDTLGEITVLGIVAITVYALLRRFRPARDSVALPEQKYF
                     QNLFDAERKRLSKEPISAHYMIVPGLIMRLLFPVIGMVAVFLLLRGHDLPGGGFVAGL
                     TFAIAFILQYMAGGARWVEARLTVLPVRWIGVGLLAAGGTGIGAALFGRPFLTSAFRY
                     VDVPLLGRIPVASALLFDIGVFALVLGATVLVLIALAHQTLRIPRAVSEATQPVGETT
                     "
     gene            complement(3109..>3331)
                     /locus_tag="VF226_04590"
     CDS             complement(3109..>3331)
                     /locus_tag="VF226_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7073297.1"
                     /translation="DFDAPDGAPVDLVFVVIWPSENRSGLLTALGGLCKKLREAPLRQ
                     ALRNATSQTEVRSLLERSTTQATPRLSEN"
BASE COUNT          776 a         1013 c         1058 g          484 t
ORIGIN      
        1 ccatcaggtt gtttctccga cgggttgcgt cgcctcactg acggcacgtg ggatgcggag
       61 ggtctggtgc gcgagagcaa tgaggaccag gaccgtcgcg ccaaggacca aggcgaacac
      121 gccgatgtcg aacaacaagg cggaggccac tggtatgcgg ccaagaagcg gaacatcgac
      181 ataccggaag gccgatgtga ggaacggacg cccgaacaat gccgcgccaa tgccggtgcc
      241 gccggctgcg agaagaccaa cgccgatcca gcgtaccggc agcacggtta ggcgcgcctc
      301 cacccagcgc gcgccgccgg ccatatactg aagaatgaag gcgatggcga acgtgagacc
      361 agcgacaaag ccaccgcccg gcagatcgtg accgcgcaga agcagaaaca ccgccaccat
      421 tccgataacc ggaaagagga gccgcatgat gaggccgggc acgatcatgt agtgggcaga
      481 aatcggctcc ttactcaacc tcttgcgctc ggcgtcgaac aggttctgga agtacttttg
      541 ctcaggcagc gccacgctgt cccgcgcggg ccggaaccgg cgcagcaagg cgtagaccgt
      601 gatggcgacg atcccaagca ccgtgatctc gccaagcgtg tcgaagcctc tgaagtcgac
      661 gaggatgaca ttgacgacgt ttcggccccc tgcttccgtg taagcgcgct cgagaaaatt
      721 ggacgaaatg ctataggtca gcggccgcgt catgacggcg taggccaggg ccccgatacc
      781 tagtccgccg gccgtcgcga tcatcaaatc gcggagccgt ctcatccggg cggttaggtc
      841 tgccggacgg tctgcttccg gttgccgctt cggcagccat ctgagaccca gcagcagaag
      901 cacggtggtt acgatctcga cgagaagctg ggtcagcgca agatcgggcg cagcccccca
      961 ggcaaaggtg atgctggtcg aaagccccgc acctcccatg aggatcactg ccgcgagccg
     1021 atggaacttt gcctgccagg cggcacccaa ggcgcaaaag catccgacga cccagacgag
     1081 cagaaaactc acgtcgactt cgctcaactt cggaaggtag agactgatgg acgctttatg
     1141 gagcggccac accgcggcga gcacgacgac gctcagcagc agcgcgatct gcggctggag
     1201 gcggttcgag ctcaaggact gtacgagacc tttggcgcca cgccatgaca gcagtgcgac
     1261 ggcacgctcg aaaatgcgct gccctttcag tcggcgcagg atcggggcgc cctcgatgtc
     1321 gccggcgaga taggcctgca gggccgtgta gactgcaacc ccgccgacca acgcaatcag
     1381 gctcatccac aacgccggcg tgaagccgtg ccacacggcc aagctgtact cgggggttgc
     1441 agacccgaga atccctctga ccgcgatgct gagaaaaccg ctgaccgtcg cctcgggcgc
     1501 cacgccgacc agaatgcacg tcagcacgag gaacgcgacc ggcagcagca tccaaagcac
     1561 gggatcgtgg ggtgtacgcg gcaggtccgt cgcaggcgga ccaaagaaca cctggtggat
     1621 cagccggagc gaataggcga cactgaacat gccggcgagt gtcgcgacat aaggcagcga
     1681 gtcgtccagc gtcgaaacaa catgctgctc gatcgattcg gcaaagaaca tctccttcga
     1741 caggaagccg ttgagcagcg ggacaccggc catcgcggca gcggcaacca tcgccaacgc
     1801 ggccgtaaaa ggcatcgcac gatagaggcc gccgaggcgg cggacgtcac gggttccggc
     1861 ctcatgatcg atgatgccgg cagccatgaa cagcgatgcc ttgaacgtgg catggttcat
     1921 gatgtggaag acggctgcca cggccgccaa cgggcttcca aggccgagga gaagcgtgat
     1981 cagcccgaga tggctgatcg tcgaataagc gagcagacct ttgagatcct gctgaaagag
     2041 agcaaggaat gcccccatga gcagcgtcgt gacgccgaga ggcgtaaaga tccagaacca
     2101 gaggtcgttc gcggccatga ccggccaaag gcgagcgagc aggaagacgc ccgctttgac
     2161 catggccgcc gaatgcagat atgccgaaac cggggtgggc gccgccatcg cccgcggcag
     2221 ccagatatga aacggaaact gcgcgctttt cgtcagcacg cctacggcaa tcagaagaag
     2281 agtcggcaga tagagctggt gggtggtgat gccgtcacct gcgcggagca cgacatcgag
     2341 atcgtaactg ccggcgatgt ggcccagcaa aagcatcccg ccgagcaggc atagacctcc
     2401 ggttgccgtc acggtcagcg tgatgcgcgc accgtcacga gcattctgat tgtggtgcca
     2461 gtaggcgatg agcagaaacg aatagaggct cgtgagctcc cagaaaaaca cgatctgcag
     2521 caggttgccc gacaggacga tgccgagcat ggctcccatg aagcccagca gaaaggagaa
     2581 gaagcgcggg accgggtccg ccggcgacat gtaatagcgc gcataaagga tgacgagcag
     2641 gccgatcgcg cacaccagga cgacgaaact ccaggcgaag ccgtcgagcc gcaggatgaa
     2701 attcaagtcc agctgaggca gccactcgaa ctccgcgcgg acgacagagc cgtttgcgat
     2761 cggtccagcc aaataaaggg caatagcgag gccggtgaag gcgacaccag ccgccagcca
     2821 tgcctccgca ttacgcgcgt tcgtggggaa aaatgctgcg acaaggctgc cgaggaatgg
     2881 caatgcgacc agaactgtga tgagaacgat gtcggtcgac atgctcggcg agcaaccttt
     2941 cgcatttgac gatgattgtt gcgccgattg tcaggatccg tcaccgcgaa cagcaagatc
     3001 ccttgaaagg cgccaggccg tcgcacccca aggttccgga aagctcgccc cggggtgcca
     3061 cacgccacaa atattaacgg ggctggcgcc aacagcaaac gctgccggtc agttttccga
     3121 gagacgtggt gtcgcttggg tggtgcttcg ctcgagaagc gaccggacct ccgtctgact
     3181 cgttgcgttg cggagcgcct gccgcaatgg cgcctcccgc agcttcttac acaatccacc
     3241 caacgctgtg agcagcccgc ttctgttttc gctgggccag attacgacga acacgaggtc
     3301 gaccggagcg ccatccgggg catcgaaatc g
//
LOCUS       DASVDU010000069         5450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1598562, whole genome shotgun sequence.
ACCESSION   DASVDU010000069 DASVDU010000000
VERSION     DASVDU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598562"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..768)
                     /locus_tag="VF226_04595"
     CDS             complement(178..768)
                     /locus_tag="VF226_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073298.1"
                     /translation="MISSLHQSLERLSSLSAWASGFALVAVALLVTVDVLCRKLLGAT
                     LAGSDEIAGYTLAGAASWSFSYCLLKRAHIRIDLLYNWTSLRVRCWLDLVALLALLLF
                     MSLFTYRAIGVLVESIQTGATSNTALVTPLWIPQTVWVTGLIYFTFTVAVVTLNSIVL
                     ISNRDYAAIRQLAGIPTIEEEVQTETEGVLDTVHAR"
     gene            complement(785..1843)
                     /locus_tag="VF226_04600"
     CDS             complement(785..1843)
                     /locus_tag="VF226_04600"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073299.1"
                     /translation="MTSVKGLLTGLLVAGTMGLSQPAAALDTREIQVVGTWGNLSQWR
                     DFESKFWNETLPADSEGQLTANAKPMDELGLSGFELMRQLKLGVFDIVHTLVSYTAND
                     VPMAGGIDLAGLVQDRAAYGKMIDAYRPALSKEFEKAYGAKILALYSFSSYSLMCNVG
                     NAPEKGLDILKGRKVRSHSISMSDFLEGVGAVGVTIPFAEVVPSIQQGVVDCAVTGVL
                     SAYQAKWWQVTNRYFSVPLGYGTVVVAANLDFWNSLSPETQAFLEAEVAKLEKAVTAN
                     SVREDELGVACYGTGPCEIGEPGNNVVITLDGADQAKLREVVTDVVLKRFAERCGPEC
                     TEIWNDSVGKALGVSATN"
     gene            complement(1914..2261)
                     /locus_tag="VF226_04605"
     CDS             complement(1914..2261)
                     /locus_tag="VF226_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX7073300.1"
                     /translation="MSADGWFDVFAYDELEQDVPEGVRVDGHYLALFRIGDGVYATSG
                     TCSHAEADLCKGYVEGDTIECPLHQARFHIPTGKVLCEPAEVGIPVYPAEVRNGRVFV
                     RLPGENGESLRRS"
     gene            complement(2309..3301)
                     /locus_tag="VF226_04610"
     CDS             complement(2309..3301)
                     /locus_tag="VF226_04610"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7073301.1"
                     /translation="MSHTQTVASSHQRRSTVAASERAQQIVGRSALRKLMVRKDWPGL
                     LFLAAHLGLIGLSGTWIYFALSTWWIVPATFVHGIFIVHLFAPLHECSHSTAFKSRWL
                     NQTTYWFCALTLGLAPLHFKLQHADHHTYTQDVERDPQMIIMGERLWGYLYYASAIPY
                     FRDIVSVLVRHALGRFSEREQRYVYPTARKQVQRQAWIMLGAYGLIAAVSVVTQSWAA
                     LTFWLIPRIVSEPVERLIRLAEHGGCDHTEDMLQNTRTILTWAPVRWLCWNMPLHTAH
                     HAAPQVPFHAMLELNRILVSHVKELRHGYPATVRHQVSMLMAKERDLRAREACG"
     gene            3488..4252
                     /locus_tag="VF226_04615"
     CDS             3488..4252
                     /locus_tag="VF226_04615"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073302.1"
                     /translation="MTVTKQKLIREARSKSGQLQIRPPRGKGTDYVYLALRSMIVRLE
                     LAPGQKIDDSAIADMLGVSRTPVREAILRLSADKLVAILPNRGAQVAPLNALELTSYF
                     EALELTHRALQHWACLRRSEKDLAKIDSARVAYENAVSIADPVAMSEGNLDFHRAIAD
                     ASGNRMFDDFVMKLSVLGMRIGWIWYRDVAEGSHEKEAARTVAEHRQIAAAIRNRAVE
                     EAERLASVHIGAFRDQVFAKLSLTLGGAVPISYRAT"
     gene            4436..>5450
                     /locus_tag="VF226_04620"
     CDS             4436..>5450
                     /locus_tag="VF226_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073303.1"
                     /translation="MALGKTDSLELNAEDEALLQEAVEHIETRVAVLRDAVPYVIDMS
                     GREATLSSYFGHSLQDKIELYELASDLGIKDFGLSNFFAFPSVTDHFIEYLLDKRLSL
                     DPFFATIAVEPIIADRPFPMGPAARQTVHYGIPNVILLIEIRPSTIASMGQTEEQALA
                     GIRSHIRHYRSLLPTETDRVGRIYVRIADPFDAYDEDPAALLRVIKLLGSEDISGILF
                     EDVKGTRFPFETYALVKLIRHHNPRPRKILAHPHRANGTEDAAVLEAILAGGDGVWSA
                     FTPQAAQGGHGSALMLLTNLMRARNPHVQQMFNTTKFQKVAERMWRIQDHLDVPPNTP
                     VVGE"
BASE COUNT         1116 a         1673 c         1626 g         1035 t
ORIGIN      
        1 tgcgctccca catgaacacg ccgatccccc ggatcaatcg ggaatccatg tagacagcgt
       61 caaggccgat gccgagcagg ccgagcgccg cagcaatggt gacccccaaa aacacgaaga
      121 tgagcagtaa ggccagtgcc agcgccgcca tggcgatgcc ctcttgttcg ctgtcgacta
      181 acgggcgtgg accgtgtcca ggactccttc ggtttcggtc tggacttcct cctcgatggt
      241 cgggatcccg gccagttgcc taatggccgc gtagtcacgg ttggagatca ggacaattga
      301 attcagcgtc accacggcca cggtgaatgt gaagtagatc agccccgtga cccagacggt
      361 ctgcgggatc cagagcggcg tcaccaaagc ggtgttagag gtcgcgccgg tctggatgga
      421 ctcgacgaga actccaatcg cccggtaggt aaaaaggctc atgaacaaga gaagggccag
      481 caatgcgacg agatcgagcc agcagcgaac tctgagactt gtccagttgt aaagcagatc
      541 tatccgtatg tgggcccgct tcagcagaca ataggagaaa gaccagcttg cggcgcccgc
      601 gagcgtgtag ccagcaatct cgtcggagcc cgcaagcgtt gcgccgagga gcttccggca
      661 cagtacgtcg acggtcacca ggagggcgac tgccaccaag gcaaagccgg aagcccaagc
      721 cgatagcgat gataggcgct cgaggctctg atggagggaa ctgatcatat taaggccact
      781 ccggtcagtt cgtggcgctg acaccgagtg ccttacctac cgagtcgttc cagatctccg
      841 tgcattccgg accgcaacgt tcggcgaacc gcttgagcac gacatccgtg acgacctccc
      901 ggagcttcgc ttgatcggct ccgtcaagcg tgatcacaac attgttgccg ggctcgccga
      961 tctcacatgg cccggtgccg tagcaggcga cgccaagctc atcctctctg accgagttcg
     1021 cggtgacagc cttctcaagc ttggcgactt cggcttcgag aaacgcctgc gtttccggcg
     1081 acaacgagtt ccagaagtcg aggttagccg cgaccacgac ggtaccgtag ccaaggggca
     1141 cgctgaagta ccggtttgtc acctgccacc acttcgcctg ataggctgac aagacgccgg
     1201 tcactgcgca atcgacgacg ccttgctgaa tggatggcac cacctcagca aacggaatgg
     1261 tgaccccgac cgcacccacg ccttccagga agtccgacat cgagatactg tggctacgga
     1321 ctttccgtcc cttgaggata tccagaccct tctcgggagc gttcccgaca ttgcacataa
     1381 gcgagtagct cgaaaagctg tagagcgcca ggatcttggc gccataagct ttttcgaact
     1441 ccttggaaag ggcgggcctg taggcgtcga tcatcttgcc atacgccgcg cgatcctgaa
     1501 ccagcccggc aagatcaatg ccgccggcca tgggcacgtc gttcgccgtg tacgagacga
     1561 gcgtgtgaac aatgtcgaag accccgagct tcagctgtcg catcagctcg aaaccggaca
     1621 ggccgagctc gtccattggc ttcgcgttgg ctgtgagttg accctccgag tcggccggca
     1681 gcgtctcgtt ccagaacttg ctttcgaagt ccctccactg ggagaggttt ccccaggtgc
     1741 cgacgacctg gatctcccgg gtatccaacg cggccgctgg ttgcgacagc cccatggtac
     1801 cggcgacgag caatcccgta agcaatcctt taacgctagt catccgcacg ttcctccctc
     1861 gtcatcttgc tgatgatctc aagtcattct atcgtggtcc gtttcgttca ttgtcagctt
     1921 cgccgtaaag attctccgtt ttcgcccggc agccgcacaa acacgcggcc gtttcgcact
     1981 tcggccggat aaacgggaat gcccacttcc gccggttcgc agagaacctt tcccgttggg
     2041 atgtggaagc gtgcctggtg cagggggcac tcgatcgtat cgccctccac gtagcccttg
     2101 caaaggtcgg cctcggcatg ggagcaggtt ccgctcgtcg catagacacc atctccgatc
     2161 cggaagagcg ccaggtaatg gccatcgacg cgcacgcctt cggggacgtc ttgttccagt
     2221 tcgtcgtacg cgaacacgtc gaaccatccg tctgcactca ccaacgcttc tcctttgtcc
     2281 ccatcacgac cggagcggct ggcttcggtc agccgcaggc ttcgcgagcc cgaaggtccc
     2341 tctcctttgc catgagcatg ctcacttggt gtctgacggt ggccgggtac ccatggcgga
     2401 gttccttcac gtgactgacc aggatgcggt tcaactcgag catggcatgg aacggcacct
     2461 gtggcgccgc gtgatgggcc gtgtgcaggg gcatgttcca gcagagccag cgaacgggcg
     2521 cccaggtaag gatggtgcgg gtgttttgca gcatatcttc ggtgtgatcg cagccgccgt
     2581 gttccgccag acggatgagc cgctccacgg gctcggacac gatgcgcgga atgagccaga
     2641 aggtgagtgc tgcccaactt tgggtcacga cggacacggc tgcgatcagg ccgtaggcgc
     2701 cgagcatgat ccaggcctgg cgctgaacct gcttgcgggc tgtggggtaa acgtagcgct
     2761 gctcgcgttc ggagaaccgt ccgagagcat gccgtaccag cacggaaacg atgtcgcgga
     2821 agtagggaat ggccgacgcg tagtagaggt aaccccacag gcgttcgccc atgatgatca
     2881 tctgaggatc acgctccacg tcctgggtgt aggtatgatg gtcggcgtgc tgcagcttga
     2941 agtgcaatgg cgccagccca agcgtcagcg cgcaaaacca gtaggtggtc tggttcagcc
     3001 accgcgactt gaaggcagtg gaatggctgc actcgtgaag cggcgcaaag aggtgaacga
     3061 taaagatgcc gtgcacgaag gtcgcaggga caatccacca cgtgctcaaa gcgaagtaaa
     3121 tccaggttcc ggacaatccg ataaggccga gatgggccgc caggaacagg agcccgggcc
     3181 aatctttgcg caccatgagc ttacgaagtg cactccggcc gacgatttgc tgcgcacgct
     3241 ccgatgccgc aaccgtggac cgtcgctggt gagacgaggc gacagtctgc gtgtggctca
     3301 tttcccgacc tctcgacctg gcaactccgt tgcgtcgccg gcggaaattg tatattattt
     3361 gtattcaaaa tgccagcggc accgcctcgc gtcaaggcag aaattgcagc ccctacagcg
     3421 agggaactgc gcagtgtaga cttgaaggct gcagagccga gtcgctcgat gcagatggag
     3481 acgagaattg acggtcacga agcagaagct gatacgcgaa gccaggagca agtctggaca
     3541 gttgcagatc cgcccgccga ggggaaaggg aaccgattac gtctatctcg cactcagaag
     3601 catgatcgtg aggctggagc tcgcgcccgg ccaaaagatt gacgactccg ccatcgctga
     3661 catgcttgga gtttcgcgga ctcccgtccg ggaagccatc ctgcggctgt ccgccgacaa
     3721 gctcgttgca attcttccga atcgcggcgc ccaggttgcg ccactgaacg cgctcgagtt
     3781 gaccagctac ttcgaggccc tcgagctgac gcacagggcg ctgcagcatt gggcctgcct
     3841 gcgccgttcc gagaaggatt tggcaaaaat agacagcgcc agagtagctt acgaaaatgc
     3901 cgtctcgatc gcagaccccg tggcaatgag cgagggtaat ctcgacttcc atcgcgccat
     3961 cgcggacgcc tcgggaaacc gcatgttcga tgacttcgtc atgaaattgt cggtgctcgg
     4021 catgcgcatc ggttggatct ggtaccgcga cgttgcggaa ggcagccacg agaaagaagc
     4081 tgcaaggaca gtggcggagc atcggcagat tgccgccgcc atccgcaacc gcgccgtcga
     4141 agaggccgag cgccttgcca gcgtccacat cggcgccttc agggaccagg tgttcgccaa
     4201 gctgagcttg acgctcggcg gcgcggtgcc gatctcctat cgcgccacct gatccgctcc
     4261 gagcctctcg aagaagcttc ggattccgct cttactttcc ggcctgcgaa tagcccatca
     4321 catgacgagg acgtcgcgca acgcgcggct acgccctctc agttgaccgt ttgtattttc
     4381 caagtatatt aatttggaat tcgatgccgc ttggcctgag ggcgagggaa cgcacatggc
     4441 gcttgggaag accgattcct tggagctgaa cgctgaggac gaagcgctgc ttcaggaggc
     4501 tgtcgagcac atcgaaaccc gcgtcgcggt cctgcgcgac gccgtgccct acgtcatcga
     4561 tatgtccgga cgggaggcga ccttgtcctc ctatttcggc cattcgctgc aggacaagat
     4621 cgagctttat gagctcgcga gcgatctcgg catcaaagac ttcggcctgt cgaacttctt
     4681 cgcgtttcca tcggtcaccg accatttcat tgagtacctg ctcgacaaga ggctctcgct
     4741 cgacccgttc tttgcgacca tagcagtgga gccaatcatc gcagaccgtc ccttcccgat
     4801 gggccctgct gcgagacaga ccgtccacta cggaattccc aacgttatcc ttctcatcga
     4861 aattcggccg tcgaccatcg cgtcgatggg acagacggag gagcaggcgc tggcgggcat
     4921 ccgctcacac attcgccatt atcgctcgct cctgccgacc gagacggacc gcgtggggag
     4981 gatctatgtc cggatcgccg atcccttcga cgcctatgat gaagatccgg cggccctgct
     5041 tcgggtcatc aagctgctgg gctcggagga catctcgggc atccttttcg aggacgttaa
     5101 gggaacccgc tttcccttcg agacctatgc gctggtcaag ctcatccggc accataaccc
     5161 caggccgcgc aagatcctcg ctcatccgca cagggcgaac ggcaccgagg acgccgcggt
     5221 gctggaggcg atcctcgcgg gcggagacgg cgtttggtcg gccttcacgc cgcaggccgc
     5281 gcagggcggt catggatcgg cgctgatgct ccttaccaac ctgatgcgcg ccaggaaccc
     5341 gcacgtccag cagatgttca acacgaccaa gttccagaaa gttgccgagc gcatgtggcg
     5401 gatccaggat cacctggacg tcccgcccaa tacgccggta gttggcgagc
//
LOCUS       DASVDU010000070         8336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1228509, whole genome shotgun sequence.
ACCESSION   DASVDU010000070 DASVDU010000000
VERSION     DASVDU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8336
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228509"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VF226_04625"
     CDS             <1..577
                     /locus_tag="VF226_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7073304.1"
                     /translation="PLVRGIRHKPAAAPSPDRIEKGAAGSMSDPAWRRGYGMLARHGF
                     HYELQAPWWHVDELLDLIAAYPETPVVINHTFLPADRSPEGIGGWRAALKRAASAPQV
                     AIKISGIGLKNRPWTLEDNRAIIRETIEIFGPDRCLFASNFPVDGLCGSFDTIYSGYK
                     QATADLPRADRLKLFHDNAIRVFRLSIPTLA"
     gene            594..1262
                     /locus_tag="VF226_04630"
     CDS             594..1262
                     /locus_tag="VF226_04630"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7073305.1"
                     /translation="MRIIDLTMPIITDHVRWKAERTVAGDTTKGDLFQVTTLKVSCHS
                     FTHVDARRHYFGDGATIEATPLEDVVGDAAVIDLMDIEPNEAVGPEKLDRRFGHVRPG
                     DMVLLKSGWDQKRSFRTKEFWLDAPYLTREAALYLKEKGIRTIAYDFPQDYVIRLLLH
                     GEVRPIEEHVTHDILLRAGVNMIEYVVNTSAITAERVLLSAAPIKIPGADGAPARVYA
                     IEGR"
     gene            1259..1753
                     /locus_tag="VF226_04635"
     CDS             1259..1753
                     /locus_tag="VF226_04635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7073306.1"
                     /translation="MTGHVIIAPEQAADIAAIRAIHTAAFGQPAEADLVDALRQGGWA
                     EISLVAKGLKEALGHVLFSRLEASMRAVALAPLAVLPDRQGSGIGSALVYDGLRSAAE
                     QGWEAVFVLGDPAYYERFGFSREAARGYDSIYAGDNFMMLELRPGSARPGAIRYPPPF
                     AGLE"
     gene            complement(1762..2274)
                     /locus_tag="VF226_04640"
     CDS             complement(1762..2274)
                     /locus_tag="VF226_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073307.1"
                     /translation="MAQAETAPSEAVGCAAGTEAPPPLPRPVQEMREAILAAVHSGDI
                     EELRVALEWNELPPHVADEPVEDPIAYWKKISGDGEGREILAALGEILECSPTALPIG
                     DDIENNLVYVWPGLAEADLSKLTPQQEVALYRLVSPAEAKAMREANRWQWWRLAIGAD
                     GTWHTFHKGK"
     gene            2384..2518
                     /locus_tag="VF226_04645"
     CDS             2384..2518
                     /locus_tag="VF226_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073308.1"
                     /translation="MLRTGMREVQMGLTFSVNAARRLVPSRARVVSVATNAGAGVPTD
                     "
     gene            2518..3213
                     /locus_tag="VF226_04650"
     CDS             2518..3213
                     /locus_tag="VF226_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX7073309.1"
                     /translation="MQFIPNPLIIPIGLLIGVLVSAPVGPVNVLCIQRAIERGFWGGV
                     AAGTGAVLGDGLIAFCAALGVGAISGAITDYRNAIQFVGGLALIGFGVKLYITRPRVI
                     TGSDSDEPHSLSEFLRDTPKTFFLTITNPGAVLGLFAIFGGVSSFVEVKSTIDAITMV
                     AAIIAGSLGWWILLSSLIGRIRHKFAYARIVQINRIAGALLFGFGAVLVGELVLQAAG
                     MLAETAYSLIATA"
     gene            complement(3203..3445)
                     /locus_tag="VF226_04655"
     CDS             complement(3203..3445)
                     /locus_tag="VF226_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073310.1"
                     /translation="MQTFCGENKSDSFKALSIILEAWDEGADQGIAPELMAYAALFTA
                     LTDLVSTFGEDAVANLARGLDARVRQGEFTICGTRQ"
     gene            complement(3572..3973)
                     /locus_tag="VF226_04660"
     CDS             complement(3572..3973)
                     /locus_tag="VF226_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEX7073311.1"
                     /translation="MLRFHTRGHTTSAVRVLAILVILLASVSALRAQETKPYDDKLMR
                     LSEILGAVHYLRELCGADEGQLWRDRMRELMDAEGTTALRRAKLTRSFNKGYMGYSRT
                     YRTCTPSAETAVKRFLAEATQLATELMRNVP"
     gene            complement(4002..4265)
                     /locus_tag="VF226_04665"
     CDS             complement(4002..4265)
                     /locus_tag="VF226_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073312.1"
                     /translation="MERLEEPARKVMEEYRRRFGHLPDLPGLEFDSEKRGRFVAALER
                     ALQRNMPLIDYEVQEFEIARLRRLRLRLKHYIARLARRRTHML"
     gene            complement(4847..5455)
                     /locus_tag="VF226_04670"
     CDS             complement(4847..5455)
                     /locus_tag="VF226_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7073313.1"
                     /translation="MRFLLVLALALAVAVPAIAAEPMPPLSGPAEPRVEPQLGEDGIY
                     HQDWFAQSFLDLREDHAEARAAGKRFAIFFEQRGCIYCAKMHKEVLSLRYINDYVREN
                     FHIVQLDLWGSREVTDFDGTVLPEKKLAERWGILFTPTVIFFKDELPDDAKWGTPLEV
                     ARMHLGMEPDTFYDMFVWVRTKTYQTDPNFQRFHIQRYEQRG"
     gene            complement(5494..6327)
                     /locus_tag="VF226_04675"
     CDS             complement(5494..6327)
                     /locus_tag="VF226_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7073314.1"
                     /translation="MDTGISTGAVVLLALALGAGGLMSGFLAGLFGIGGGAILVPVLY
                     EVWTAVGIPPEIRMHMAVGTSLAVMIPTTTKSLMSHKARGGVDMSFLKRLAGPVIVGV
                     LVGALIASLSPTAVLKWIWAIFASTMAARMLWGQDNWRLGNDIPKSRLVELYGVFVGL
                     ISTLLSIGGGAFITMLMTLYGRPIHQAVGTSSGFGPLISIPGAIGFVVAGWGAAGLPV
                     GSVGYVNLIGAAVVIPASVYAAPWGARLAHGISRRKLEIAFGCFLAVIALRYITDLVL
                     G"
     gene            complement(6338..7360)
                     /gene="dusA"
                     /locus_tag="VF226_04680"
     CDS             complement(6338..7360)
                     /gene="dusA"
                     /locus_tag="VF226_04680"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEX7073315.1"
                     /translation="MTDLDNQVDLSCSRVSVAPMMDWTDRHCRFFHRLLTRHALLYTE
                     MVTAEAVIHGDRQRLLGFDPAEHPVALQLGGSDPERLATAARIGADFGYDEINLNVGC
                     PSDRVQEGRFGACLMAEPDLVARCVAAMRDACDVPVTVKCRIGIDDQDSEADLEKFVL
                     TVASAGCTRFIVHARKAWLQGLSPKENRDVPPLDYDRVYRLKATHPELTIIINGGIRS
                     LDEAEAHLGRVDGVMLGRAAYQTPYLLADVDRRFFGAGEPQITRREVIEKLVPYIERH
                     LARGGKLNNVTRHLLGLYHAEPGGRLFRRHLSGADARDGDGLEPLMRALAAVEQADPQ
                     SRRAAA"
BASE COUNT         1573 a         2626 c         2615 g         1522 t
ORIGIN      
        1 cccgctcgtg cgcggcatcc gccacaaacc ggccgcggcg ccttcgccgg acaggatcga
       61 gaagggggcg gcaggctcca tgtccgatcc tgcctggcgg cgcggctatg gaatgctcgc
      121 ccgccacggc ttccattacg agctgcaggc gccctggtgg cacgtcgacg agctgctcga
      181 cctcattgcc gcttatcctg agacgccggt ggtcatcaac cacaccttcc tgccggcgga
      241 tcgcagcccc gagggaatcg gcggatggcg ggcggcgctc aagcgggcgg catcggcccc
      301 acaggtagcg atcaagatct ccgggatcgg cctcaagaac cggccctgga cgctggagga
      361 caaccgcgca atcatccgcg agacgatcga gatcttcggc cccgatcggt gtctcttcgc
      421 ctccaatttc ccggtcgacg gcttgtgcgg cagcttcgat accatctatt caggctacaa
      481 gcaggccacg gcggacctcc cccgcgcgga ccggctcaag ctctttcacg acaatgccat
      541 ccgcgtcttt cggctctcga taccgacgct cgcctgacgg attgaaacag cacatgcgca
      601 tcatcgactt gaccatgccc atcatcaccg atcacgtccg ctggaaagcc gagcggacgg
      661 tggcggggga tacgaccaaa ggcgatctct tccaggtgac caccctcaag gtctcctgcc
      721 attccttcac ccacgtcgat gcacggcggc actatttcgg cgatggcgca accatcgagg
      781 caacgccgct ggaggacgtc gtcggcgatg ccgccgtgat cgatctgatg gacatcgagc
      841 ccaacgaggc ggtcgggccg gagaagctgg accggcgatt cggccatgtc cgtcccggcg
      901 acatggtcct gttgaagtcg ggctgggatc agaagaggtc gttcaggacc aaggaattct
      961 ggttggatgc gccctatctc acgcgagaag ctgcgctcta tctgaaagag aagggtatcc
     1021 gcaccatcgc ctacgatttc ccgcaggatt acgtcatccg tctgctgctg cacggcgagg
     1081 ttcgcccgat cgaggagcat gtgacgcacg acatcctgct gcgtgccggc gtgaacatga
     1141 tcgaatacgt ggtcaacacc agcgcgatca ctgccgagcg cgtgctgctt tcggccgccc
     1201 cgatcaagat cccgggcgcc gacggcgcgc cagcccgcgt ctatgccatc gagggccggt
     1261 gacaggtcac gtcataattg cgccggagca ggcggccgac atcgcagcga tccgcgccat
     1321 ccataccgcc gccttcgggc agcctgccga ggccgatctg gtcgatgctc tgcggcaggg
     1381 cggctgggct gaaatctcgc tcgtggcgaa gggcctgaag gaagcccttg ggcacgtgct
     1441 gttcagccga ctcgaggcat ccatgcgcgc cgttgcgctt gcgccgctgg cagtcttgcc
     1501 cgaccggcag gggtccggga tcggcagcgc gctggtctac gatggcttga gatctgcggc
     1561 tgagcagggc tgggaggctg tcttcgttct cggcgatccg gcctattacg agcggttcgg
     1621 gttcagccgg gaagcggcac gcggatacga cagcatctat gcgggcgata acttcatgat
     1681 gctcgagctg cgacccgggt ccgcgcgccc gggggccatt cgctatccac cgccgttcgc
     1741 cggtctcgaa tagtgattgc ctcacttccc cttgtggaac gtatgccagg tcccgtcggc
     1801 gccgatggcg aggcgccacc actgccagcg gttggcttcg cgcatggcct tcgcttcggc
     1861 cgggctgacg agacggtaga gcgcgacctc ctgctggggt gtcagcttcg acagatcggc
     1921 ttcggccagt ccgggccaga cgtagacgag attgttctcg atatcgtcgc cgatcggcag
     1981 ggccgtgggg gagcattcga ggatctcgcc caacgcggcg aggatctcgc ggccctcgcc
     2041 gtccccggag atcttcttcc agtaggcgat cgggtcctcg actggctcgt cggcaacgtg
     2101 agggggaagc tcgttccact cgagggccac ccgcagctcc tcgatgtcgc cactatgaac
     2161 cgcggcgagg atcgcctcgc gcatctcctg gacgggtcga ggcaggggag ggggtgcttc
     2221 ggtcccggcg gcacaaccga cagcctcgga gggcgcggtt tcagcttgcg ccatgaggac
     2281 gcctaacggc gctaaccatt ggacaataca ggtcagcgaa actaccaata agtaatcgcg
     2341 aaaagtgatt tgggcgcgta accaacccat aaggagagct ccgttgctgc gcactggcat
     2401 gcgcgaagtt caaatgggct tgacgttttc cgtcaatgcc gcgcgccggc ttgtgccgag
     2461 cagggcaaga gtcgtttccg tcgccaccaa cgccggagcg ggcgtgccga ccgactgatg
     2521 cagttcatac caaatccgct catcatcccc atcggcctcc tcataggtgt ccttgtgtct
     2581 gcgcccgtcg ggccggtgaa cgtgctctgc atccagcgtg cgatcgaaag gggcttctgg
     2641 ggcggtgtcg cggcggggac aggcgcggtg ctgggagacg ggctgatcgc cttctgcgcg
     2701 gcgctcggcg tcggagcgat ctcgggcgcc atcaccgatt accggaacgc cattcagttc
     2761 gtcggcgggc tggcgcttat cgggttcggg gtgaagctct atattacccg cccgcgcgtc
     2821 atcaccggca gcgactcgga tgagccgcat tcgctctccg aattcctgcg ggacacacca
     2881 aagacgttct tcctgaccat caccaatccc ggcgcagtgc tcggcctctt tgccatcttc
     2941 ggcggcgtct ccagcttcgt cgaggtcaag tcgaccatcg atgccatcac gatggtggcc
     3001 gccatcatcg caggcagcct tggctggtgg atcctgctgt cttcgctgat cgggcgcata
     3061 cggcacaagt ttgcgtatgc ccgcatcgtg cagatcaacc gaattgctgg cgcgctgctg
     3121 ttcggcttcg gcgcggtcct ggtcggtgag ctcgtgctgc aggcagccgg catgctggca
     3181 gagacggcct acagcctcat cgctactgcc tagtcccgca aatcgtgaat tcgccctggc
     3241 gcacgcgggc atcgagcccc cgcgccagat tggcgacggc atcctcccca aatgtcgaaa
     3301 ccaggtcggt gagagccgta aatagcgctg cgtaggccat gagctcaggc gcgataccct
     3361 ggtcggctcc ctcgtcccag gcctccagta tgatgctcag cgccttgaag ctgtcgctct
     3421 tgttctctcc gcagaacgtc tgcatgattg ctctcgtcct ctgcacgtgg ctgtgcacgt
     3481 ccccacaaac gcaagccgta gcacagggcg cgcccgcgag tagcaaccac aacaattaac
     3541 agtaaatctc ggaaaatgcg gattgatgcc ctcagggcac attgcgcatc agctcggtcg
     3601 cgagctgggt cgcctcggcg aggaagcgct tgactgccgt ctcggcagag ggcgtgcacg
     3661 tgcggtaagt gcggctgtag cccatgtagc ccttgttgaa gctgcgggtc agcttcgccc
     3721 ggcgcagggc ggtcgtgcct tccgcgtcca tcagctcccg catccggtcg cgccagagct
     3781 ggccctcgtc ggcgccgcag agctcccgga gatagtggac ggcgccgagt atctcggaaa
     3841 gccgcatgag cttgtcgtcg tagggctttg tctcctgggc gcgaagggcg ctgacggacg
     3901 caagcaggat caccaggatg gccaggactc ggactgccga ggtagtgtgg ccgcgggtgt
     3961 gaaaccgaag catgcaacct ttccttgaat gcggatccgg ttcatagcat gtgcgttcgg
     4021 cgacgcgcca gccgcgcgat gtagtgcttg agcctcaggc gcagccggcg caggcgcgcg
     4081 atctcgaact cctgcacctc gtagtcgatc aacggcatat tccgctgcag cgcccgctcg
     4141 agggccgcca cgaaacggcc acgcttctct gaatcgaatt cgagcccggg cagatcggga
     4201 agatggccga atcgccgccg gtactcctcc atgactttgc gcgccggttc ctcgagacgt
     4261 tccatagaaa aacactgatc cacccgtggg cctgtctcca ggattgccgc cctgggtgaa
     4321 tgctcgatgt cagaattatt tcggtttacg aaagaacatg gcccctttgc ggatgcaact
     4381 ttcgtcttct cgacagcaat ggacaattca gaggcggtat cgccaacata gatgaaagaa
     4441 aatcattcac aagcgagtga actgatgaat tggcccttac tccttgtgga ggatggtgcg
     4501 gtgccgcaag gggcgcaata aaggggaacc cgctcgggtc ccccgcgttt ctatttgtgc
     4561 ttgacgctga agaggtggag aaatatcgct gtgatttcaa gaactatcac ccaccacttc
     4621 aaccagtcgc aggatttgaa gttggcactc atgtgggtct ctccgtcctg atgggtgccg
     4681 accgacggcg gagcggcaac tccgccgtca cccgtttggc catcggctta accgaaaact
     4741 cacaaatcgc ccaattggtt cgacaaatga tcgatttgtg atcggctgcg cacggcgtag
     4801 gctgctgaaa tagctgaacg cgctcggacg tgaggcaccg gccggcctat ccgcgttgct
     4861 cgtagcgctg gatgtggaac cgctgaaagt tcgggtccgt ctggtaggtc ttcgttcgga
     4921 cccacacgaa catgtcgtag aaggtgtcgg gttccatgcc gagatgcata cgtgcaacct
     4981 cgagcggtgt gccccacttg gcgtcgtctg gaagctcgtc cttgaagaag atcaccgtcg
     5041 gcgtgaacag aatcccccaa cgctcggcga gctttttctc gggcagaacg gtgccgtcga
     5101 aatcggtcac ctcgcgcgag ccccagagat cgagctgcac gatgtggaag ttctcgcgca
     5161 cgtagtcgtt gatgtagcgg agcgagagca cctccttgtg cattttcgcg cagtagatgc
     5221 agccgcgctg ctcgaagaag atggcgaacc gtttgccggc cgccctggct tcggcgtggt
     5281 cctcccggag atccaggaac gattgggcga accagtcctg atgatagatg ccgtcctcgc
     5341 ccagttgcgg ttcgacgcga ggctcagcgg gaccggaaag cggcggcatg ggctccgccg
     5401 cgatggccgg cacggcaaca gccagggcca aggccaaaac cagcaggaag cgcatcggat
     5461 ctactcctcc gtgaacgcgt ctatcgactc aaatcagcct agcaccagat cggtgatgta
     5521 gcgtagcgcg atcaccgcga gaaagcagcc gaatgcgatc tcgagcttgc gcctcgagat
     5581 gccgtgggcc aggcgcgcgc cccagggggc ggcatagacg ctcgccggaa tgacgacggc
     5641 ggcgccgatc aggttgacgt agccgaccga gccgaccggc aggccggccg cgccccagcc
     5701 tgcgaccacg aatccgatgg cgcccgggat agaaatgagc ggtccgaaac ccgaggacgt
     5761 gccgacggct tgatgaatcg gccggccata aagcgtcatg agcatggtta tgaaggcccc
     5821 gccgccgatg ctcaaaaggg tggaaatcag gccgacgaac acgccgtaga gctcgaccag
     5881 acgactcttg ggaatgtcgt tgccgagccg ccagttatcc tggccccaca gcatgcgggc
     5941 ggccatggtc gaggcgaaga tcgcccagat ccacttgagc acggcggtgg gactgaggct
     6001 ggcgatcaaa gcccccacga gcacgccgac gatcactggc ccggcaaggc gcttcaggaa
     6061 gctcatgtcg actccgccgc gcgccttgtg cgacatgagc gatttcgtcg tggtggggat
     6121 catgacggca agcgaggtgc cgacggccat gtgcatgcgg atttcgggag gaatgcccac
     6181 cgccgtccag acctcgtaca gcacgggaac caggatggcg ccgccgccga tgccgaacaa
     6241 tcccgccaga aatcccgaca taaggccgcc agcacccaac gccagcgcca gcagcaccac
     6301 cgcgccagtc gaaattcccg tgtccatgcg aattgtccta tgcggccgcg cggcgcgatt
     6361 gtggatcggc ctgctccacg gccgcaagtg cccgcatcag cggctccaat ccgtcgccgt
     6421 cgcgcgcatc ggcgccgctc agatgccggc ggaaaagacg ccccccgggc tcggcatgat
     6481 agagtccgag caggtgcctg gtcacgttgt tcagtttgcc gccgcgtgcc aagtgccgct
     6541 cgatgtaggg gaccagcttt tcgatcacct cgcggcgcgt gatttgcggc tcgcccgcgc
     6601 cgaaaaaccg gcgatccacg tcggccagaa gatacggcgt ctggtaggcg gcgcggccga
     6661 gcatgacgcc gtcaacacgg ccaagatgcg cttccgcctc atcgagcgag cggatgccgc
     6721 cgttgatgat gatggtcagc tccgggtggg tcgccttcag gcggtagaca cggtcgtaat
     6781 cgagcggcgg cacatcgcgg ttttccttcg gcgagaggcc ctggagccaa gccttgcgcg
     6841 cgtgcacgat gaatctcgtg cagcccgcgg acgcgacggt taaaacgaac ttctcgagat
     6901 cggcctcact gtcctggtcg tcgatgccga ttcggcactt caccgtgacc ggcacatcgc
     6961 acgcatcccg catcgccgcg acgcatctcg caacgaggtc cggctccgcc atcaggcagg
     7021 caccgaagcg gccttcctgc acccgatccg acgggcagcc gacattgaga ttgatttcat
     7081 cgtagccgaa atccgcgccg atccgcgccg cggtggcgag ccgctcggga tcggagccgc
     7141 cgagctgcag cgccaccggg tgctccgccg gatcgaagcc gagcagcctc tggcggtcgc
     7201 catgaatgac cgcttcggcg gtgaccatct ccgtatagag caaggcatgc cgagtcagca
     7261 ggcggtggaa gaaccggcag tgccggtccg tccaatccat catgggcgcc acgctgactc
     7321 tgctacagga aagatcaact tgattgtcaa gatcagtcac ttacgcctcg tccttcgtgg
     7381 gccttggctc caattcgacc ccctgattgc ctccactctt atccctcgtc cggtgcgata
     7441 gatgtgctag ctgcgcgagc tgcggaaggt gctcgccgtt tatgtagcaa atctggaggg
     7501 cttattggac catcacggcg cggaaccaaa ggacgggacg attgggttca cgctacagat
     7561 caggatcaaa agaggcggtc aatccgtctg ctgacaatga caacttccca ccaccaggga
     7621 gccagaacca gccgcacccg ggatcattta catcttgccc gccgcgctga ccatcggagg
     7681 gggctggtgg gtaggaacaa gctctgcagg aacttaggtg aagcggcttc atcgtctgga
     7741 gggccaggaa ggtgccgcgc ttcatgtcga gcaggtccga aacgcagtag cgccgtgtcg
     7801 gccaatcccg ctgcttcctg atggaatcac gccggtactt tagcgccaaa tcctggctgc
     7861 ctgggatacg ctcaaacatg gcacctgcca cagcgagatc gcctgttgcc agatccatga
     7921 tgcagttgat gcccgcagca tcgttgatcg ggctgtcggt cttgatgcat ggcctctagc
     7981 gccacattga cgtccctcgc cggctgccgc cactcaggcc gcgagagctc cgagatagtg
     8041 cgtgacgtct gggtcacgcg tcatcatgcg aaagtcggaa aacgatcgga cctggccgcg
     8101 gttgaggaaa ataaactggt cgcaaatctc ccgcaggata tcaagttgga aggtcgccgt
     8161 cgggtgcagg cagacgaaaa cgaggcgacc ctcgctccgc agccgacgaa aaaatcgagc
     8221 atgaagccga taaggccatc ctgcgtgtta aactgcggct cgtcgaatag gtggacaagc
     8281 gggtaggcgc tgtgcgctgg cttaaggaga aagctcggct tcgtcttgct gaaacg
//
LOCUS       DASVDU010000071         9681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_625255, whole genome shotgun sequence.
ACCESSION   DASVDU010000071 DASVDU010000000
VERSION     DASVDU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625255"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VF226_04685"
     CDS             complement(<1..1470)
                     /locus_tag="VF226_04685"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEX7073316.1"
                     /translation="MLAKLVAAAKPLGIDILPVFTPALNLKTESTEQLYDRAYRMAVA
                     LISQFKDDIRVWELGNELENFAIIRACEMRDNGEQYNCSWGPAGGVDPLDYYGPRWAK
                     VSAVLKGLSDGAMAVDPTIRKAIGTAGWGHLGAFERMHQDGIKWDISVWHTYGQDVEW
                     ALKALAKYNRPVWVTEFNNPLGSKNSEDEQVHGLVKQMKRMRELQKSYDIEAAHIYEL
                     LDESYWAPDYEAYMGLVRLERKDDGRWKIGERKPAYEAVKQLIAHAEDQSVQPPGGVV
                     EKQVQSFARKNCNLEQLARTGQPITAEAQVEYAYCLILDREADGGGLESWTASLQSGT
                     SGSQLILAMLHSDEFSEKHAVSALDNSGFVSLVHRVLLDREPDRGNRTALVDQLEGGK
                     VGRVDIVKGILASDEFRSKHPILFASVATPTARGTRPTEIKRNCDLGAFDPVQSRVEQ
                     RAAYIHCLVFGQVGNTKDRDAWVANIGRGTPPWEMLATLL"
     gene            complement(2123..2371)
                     /locus_tag="VF226_04690"
     CDS             complement(2123..2371)
                     /locus_tag="VF226_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073317.1"
                     /translation="MADRQDLIRAVDLALAGDWDAAHKIVQKHEDDADACWIHAVLHK
                     MEGDAANSRYWYRRTMHAYEAYPDPRQELQAIKAALTY"
     gene            complement(2350..3045)
                     /gene="queC"
                     /locus_tag="VF226_04695"
     CDS             complement(2350..3045)
                     /gene="queC"
                     /locus_tag="VF226_04695"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX7073318.1"
                     /translation="MRALVLFSGGQDSTTCLAWALSRYDHVETIGFAYGQRHEVELIA
                     RRRVLAGLRADFPEWGQRLGEDRVLDLAEFGAISDTALTREAEIEMTAAGLPSTFVPG
                     RNLVFLTYAAAVGYRRRIPVLVGGMSETDYSGYPDCRNETLQTLARALSLGLDWAVAI
                     ETPLMWISKAGTWALAQELGGDRLIELIVEETHTCYLGDRSKRHPWGYGCGTCPACEL
                     RRKGWDEWQTARI"
     gene            complement(3050..3409)
                     /locus_tag="VF226_04700"
     CDS             complement(3050..3409)
                     /locus_tag="VF226_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7073319.1"
                     /translation="MSEARTYTGGCHCGKVRYEVETSLTPVLDCNCSICQKRGALWTY
                     VDPPAFKLLTGEGELTDYQFNKHNIHHLFCATCGVASFARGSSTEGGEGIAINVRCLD
                     EIDLASLTLTPFDGRSL"
     gene            3562..4848
                     /gene="eno"
                     /locus_tag="VF226_04705"
     CDS             3562..4848
                     /gene="eno"
                     /locus_tag="VF226_04705"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7073320.1"
                     /translation="MTAIIDITGREILDSRGNPTIEVEVVLEDGSMGRAAVPSGASTG
                     AHEAVELRDGDPARYNGKGVLKAVDAVNGEIYNALRDHEAEDQRAIDKALIDLDGTPD
                     KGRLGANAILGVSLAVAKAAADACTLPLFRYIGGTSAHILPVPMMNIINGGAHADNPI
                     DIQEFMIMPVSAPTCADAIRMGAEVFHTLRKALKDAGHNTNVGDEGGFAPNLRSTDEA
                     LGFIMRAIEKAGYKPGDDVMLALDCAATEFFKDGKYDMAGEGKTLDSEAMTRYLADLV
                     SRYPVVSIEDGMAEDDWQGWKQLTAELGERVQLVGDDLFVTNTKRLAQGIAEGVGNSI
                     LVKVNQIGTLTETLDAIAMAQRAAYTAVISHRSGETEDATIADLAVATNAGQIKTGSL
                     SRSDRLAKYNQLIRIEEELGGVACYAGRSVLRKRAS"
     gene            4966..6183
                     /gene="chrA"
                     /locus_tag="VF226_04710"
     CDS             4966..6183
                     /gene="chrA"
                     /locus_tag="VF226_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006700571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX7073321.1"
                     /translation="MDRNETAGSTGKPGSAAEVFRVFLGLGLTSFGGPVAHIGYFREM
                     FVVRRKWLSENAFADLVALCQFLPGPASSQVGMAIGLLRAGYTGLLAAWLAFTLPSAI
                     VLVAFAYGAGTLAQAAGTGWISGLKAAAVAVVANAVLGMARSLAPDRERATIAVIGMI
                     GAMLIPFAFGQVLVIAIGGLIGLVWLSAKVGPVEEDASLTIAVSQRAAILFLAIFFAL
                     LVLLPFLASVSGNGLLRLIDGFYRAGSLVFGGGHVVLPLLQAEVVETGMVGREAFLAG
                     YGAAQAVPGPLFTFAAYLGAVMQQAPNGLTGAGIALVAIFIPSALLILGGLPFWDRLR
                     SAPLAQRALAGVNAAVVGLLAAALYDPVFADGITSHTAMALAAASFVALAAWQVPAWA
                     VVIAAGVAGFAVL"
     gene            6324..6629
                     /locus_tag="VF226_04715"
     CDS             6324..6629
                     /locus_tag="VF226_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7073322.1"
                     /translation="MRRTRQHILVLLGCLSMTAYFVHHTITGKHGLEARSRLVERSKA
                     LEREIAGLDAVHAELMRDVLLLRPDPPHPDLIEEIATGVLGMARPGDRIVLVGSDVP"
     gene            6767..7843
                     /gene="pdhA"
                     /locus_tag="VF226_04720"
     CDS             6767..7843
                     /gene="pdhA"
                     /locus_tag="VF226_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX7073323.1"
                     /translation="MAPADKARGSTGARSAKTAARNGSGTAVAEISPTGGDVADFTRE
                     EELHAYREMLLIRRFEEKAGQLYGMGFIGGFCHLYIGQEAVVVGMQMCAREGDQVITT
                     YRDHGHMLATGMDPKGVMAELTGRRGGYSKGKGGSMHMFSVEKNFYGGHGIVGANVPL
                     GAGLAFANKYRGNDNVTFCYFGDGAANQGQVYETFNMSKLWKLPILFIIENNKYAMGT
                     SVERSAATTDFSQRGLSFDIPGEQVDGMDVRAVKAAGEKAVKWAREGNGPYILEMLTY
                     RYRGHSMSDPAKYRTREEVERVREERDPIERVRARMLEKKYASEEELKAIDAEIRGII
                     NAAAEFAQNDPEPDPSELWTDVLR"
     gene            7941..9347
                     /locus_tag="VF226_04725"
     CDS             7941..9347
                     /locus_tag="VF226_04725"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX7073324.1"
                     /translation="MPTQVLMPALSPTMEEGKLAKWLVKEGDEVKSGMVIAEIETDKA
                     TMEVEAVDEGRLDRILVPAGTEGVKVNTPIAVILQDGEAPGTPVPGSPQAPRDLKQAD
                     AQALPAQARPAAPPGGEPVAPPQPPPARGVGEEELPPDTEMITQTMREALRDAMAEEM
                     RRDPDVFLMGEEVAQYQGAYKVSQGLLDEFGSRRVIDTPITEQGFAGIGIGAGFAGLK
                     PIVEFMTWNFAMQAIDQIVNSAAKTLYMSGGQMGCSIVFRGPNGAAARVAAQHSQEFS
                     AWYAHVPGLKVVAPSNPSDAKGLLKSAIRDPNPIIFLENEILYGASGLVPKVDDWLVP
                     IGKARIARPGNHVTIVSWSRGMVYALDAAERLAKEGIEAEVIDLRTLRPLDLETVLTS
                     VRKTNRIVTVEESWPVCSVGSEICAQVAIQAFDYLDAPPAKISGADVPMPYAANLERL
                     ALPNAEQVVEAAKRVLYV"
     gene            9455..>9681
                     /locus_tag="VF226_04730"
     CDS             9455..>9681
                     /locus_tag="VF226_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073325.1"
                     /translation="MLSLSRPASEMRVRACQCGFCRRHGTRTIADPAGSVVISTASPD
                     QIRRYRFGLRTADYLLCATCGSYIGAVQAEP"
BASE COUNT         1760 a         3015 c         3100 g         1806 t
ORIGIN      
        1 caagagcgtt gccagcatct cccagggagg cgttccccga ccgatattcg cgacccacgc
       61 atcgcggtct ttggtattgc ccacttggcc gaataccagg cagtggatat aggccgcccg
      121 ttgttcgacg cgtgactgga caggatcaaa ggcgcccagg tcgcagttcc tctttatctc
      181 tgtgggccgt gtcccacggg cagtgggggt tgcaaccgac gcaaacagga tcgggtgttt
      241 ggagcggaat tcgtcggacg cgaggatgcc tttcacaata tccacgcggc ccaccttgcc
      301 accctccagt tgatcgacga gcgctgtgcg gttgcctcgg tcgggctcgc gatcgagcag
      361 cactctatgt accaatgaca cgaagcccga gttgtcgagt gccgaaaccg catgtttctc
      421 agaaaactca tccgaatgga gcattgccaa aatcagttga gacccagaag tcccactttg
      481 gagggaagcc gtccaactct cgagacctcc gccatcagcc tctcggtcaa ggatcaggca
      541 ataggcgtat tcgacttggg cctctgcggt gattggctgc cctgtccttg cgagctgttc
      601 gagattgcag tttttgcgag cgaaagattg aacctgtttt tccaccacgc cgcctggcgg
      661 ttgcacagat tgatcctcgg cgtgggcgat gagctgttta actgcctcgt aagctggttt
      721 ccgctcgcct atcttccagc gaccgtcatc ttttctctcc agacgaacca gccccatgta
      781 ggcctcgtag tccggggccc agtaggactc gtccagaagc tcgtatatgt gcgctgcctc
      841 gatatcgtag ctcttctgca actcccgcat gcgcttcatc tgctttacga gaccatggac
      901 ttgctcgtct tcactgttct tgctgccgag gggattattg aactctgtca cccataccgg
      961 tcggttatat tttgcgagcg ccttcaaggc ccattccaca tcttggccat aggtgtgcca
     1021 aacggagata tcccacttga ttccgtcctg atgcatgcgc tcgaaagcgc cgagatgccc
     1081 ccaacctgcc gtaccgatgg ctttccgaat tgtcggatca acggccatcg ctccgtctga
     1141 gagccccttg agcacagcgc tcaccttggc ccaacgcggc ccatagtaat cgagcgggtc
     1201 aacacctccg gccgggcccc atgagcaatt gtactgttcg ccgttgtcgc gcatttcgca
     1261 ggctctgatg atggcgaagt tctcgagctc atttccgagc tcccagacgc ggatatcgtc
     1321 tttgaactgc gagatgagcg cgaccgccat cctgtacgcc ctgtcgtaga gctgctcggt
     1381 gctctcggtc ttgaggttga gggctggcgt gaagaccgga aggatgtcga tgccgagcgg
     1441 cttggcagcg gctacgagtt tcgccagcaa gggcgcttgg ctcggaacgg aaatgttcac
     1501 cctgtaggac ttcattccga gatccttcac cagctcgagc tgttgctgaa agggaatgcc
     1561 cggatatgcg gtgaacgggt gaccgtttac gccccaaatg aggtcgctct tggcggatgt
     1621 cggtgctgcc aggatgacca tgagcccagc gaggattgct agcttcagcg cgcgcatagc
     1681 accttctcgt ttcaaggagg tgggctttga tggcagcaca ggtatacaac cggcgaattg
     1741 caggcacacc aaacactcgc aagtagcgtt aagacgtgag caggcgtcgc agatggttcg
     1801 gcaatcgatg cgcctgaaat tggcctattg gcctccctaa tcgaagaatt gagtaatcga
     1861 caaatctgat tggtggagcc gagtctgcgg cccgctctcc ccattcgcaa atcagctcga
     1921 cctggttcta aatcgcgcca ggaaatccag gcggaactat catcgggagc ttcagctacc
     1981 ggaatgcagg atgattgtac ccagatgagt gctaaatagg gtccctggaa agcattcaga
     2041 acagggcgaa ggccattcag cggaaacgcc gttccgccgt tgaaacggag tcgtttggaa
     2101 ttttcctgat cagcgcactt gcttagtacg tcagcgccgc cttgatcgcc tgcagctcct
     2161 ggcgggggtc ggggtaggcc tcgtacgcat gcatcgtccg gcgataccag tagcggctgt
     2221 tcgcagcatc gccttccatc ttgtgcagga cggcgtggat ccagcacgca tccgcgtcgt
     2281 cttcatgttt ctggacgatt ttatgagccg catcccagtc gcccgcgagg gcgaggtcga
     2341 ctgcgcgaat caaatcctgg cggtctgcca ttcgtcccat cctttccggc gcagctcgca
     2401 agcagggcag gtgccgcagc cgtagcccca cggatggcgc ttgctgcgat cgcccagata
     2461 acaggtgtgg gtttcctcga cgatcaactc gatgagccgg tcaccgccga gctcctgcgc
     2521 cagcgcccaa gtgccggcct tgctgatcca catcaacggc gtttcgatcg ctactgccca
     2581 gtcgagaccg aggctgaggg cgcgggcgag cgtctgaagc gtttcgttgc ggcagtcggg
     2641 atagccggaa tagtccgttt cgctcattcc tccgaccagc acgggaatgc ggcgtcggta
     2701 gcccaccgcc gcggcatagg tgagaaacac gaggttgcgg ccaggcacga aggtcgatgg
     2761 caggccggct gccgtcatct cgatttcggc ttcgcgggtc aatgccgtgt cgctgatggc
     2821 accaaattcg gcgagatcga gtacgcgatc ctcgcccagc cgctgacccc attccggaaa
     2881 gtcggcgcgc aaccccgcca gcacccgcct ccgcgcaatc agctcgacct catggcgctg
     2941 gccgtaggca aacccgatcg tctcgacgtg gtcgtagcgc gaaagcgccc aggcgaggca
     3001 cgtcgtcgag tcctgtcctc ccgagaacag gaccagcgct cgcatcggct cacaggctcc
     3061 ggccgtcgaa cggcgtcagc gtcaggctgg caaggtcgat ttcgtcgagg cagcgcacgt
     3121 tgatggcaat accttcaccg ccctcggtgc tcgatccgcg tgcgaacgag gcgacgccgc
     3181 acgtcgcgca gaaaaggtgg tggatgttgt gcttgttgaa ctgatagtct gtcagctcgc
     3241 cttcgccggt gagcagcttg aacgccggcg gatcgacata ggtccacagc gcgccgcgct
     3301 tctggcagat ggagcagttg cagtcgagca cgggggtaag cgaggtctcc acctcgtagc
     3361 gcacctttcc gcagtggcaa ccgcccgtgt aggtcctggc ttcgctcatg gctttcgctc
     3421 ccgaggttgt cgcaggcttg tgtaccgcgc gatagcctct cagcgacaca ggtgcaagcg
     3481 gctcttgtca atggacatgg gtgggctaga acccgcgccc gagttgccgc gtaatccccc
     3541 aaactcgagc aggaggcctt catgacagcc atcatcgata tcaccggccg ggagatcctc
     3601 gacagtcgcg gcaatccgac gatcgaggtc gaggtcgtgc tggaggacgg ctccatgggc
     3661 cgtgcggccg tgccctcggg cgcatcgacg ggtgctcacg aggcggtcga gctgcgggat
     3721 ggcgatccgg cgcgctacaa tggcaagggc gtcctcaagg ccgtcgacgc ggtcaacggc
     3781 gagatctaca acgcgttgcg cgaccatgag gccgaggacc agcgcgccat cgacaaggcg
     3841 ctgatcgatc tcgatgggac gccggacaag ggccggctcg gcgccaacgc catcctgggc
     3901 gtgtcgctgg cggtcgccaa ggctgctgcg gacgcctgca cccttccgct cttccggtac
     3961 atcggaggga cgtcagcgca catcctgccg gtgccgatga tgaacatcat caacggcggc
     4021 gctcacgccg ataatccgat cgacatccag gagttcatga tcatgccggt ctcggcgccg
     4081 acctgcgccg acgccattcg catgggagcg gaggtgttcc acaccctgcg caaggcgctc
     4141 aaggatgccg gccacaatac caacgtcggc gacgagggcg gtttcgcgcc gaatctgcgg
     4201 tctacggacg aggcgctggg cttcatcatg cgcgccatcg aaaaggcagg ctacaagccg
     4261 ggcgacgacg tcatgctggc gctcgactgc gcggcgacgg agttcttcaa agacggaaaa
     4321 tacgacatgg ccggcgaggg caagacgctc gattcggaag ccatgacccg ctatctcgcc
     4381 gacctcgtca gccgctaccc ggtggtatcc atcgaggacg ggatggcgga ggacgactgg
     4441 cagggctgga agcagctcac cgcagagctc ggcgagcgcg ttcaactcgt cggcgacgac
     4501 ctgttcgtca ccaacacgaa gcggctggcg caaggcatcg ccgagggcgt gggcaactcg
     4561 atcctcgtca aggtcaacca gatcggtacg ctgaccgaga cgctggacgc catcgccatg
     4621 gcgcagcggg cagcctacac ggccgtcatc tctcatcgct ctggggagac cgaggacgcc
     4681 acgatcgcgg accttgccgt tgccaccaac gccggccaga tcaagaccgg atctctcagc
     4741 cgctcagacc ggctcgccaa gtacaaccaa ctcatccgca tcgaggagga gctcggcggg
     4801 gtcgcctgct acgctggccg cagcgtgctg cgcaaacgcg cgtcttgatg gcaattgcca
     4861 gggccgtcca cggccgcggc gtggtgcagc tgatccgctg agctaatgtg gcggactggc
     4921 gaccgcgttg gtcgggcgcc gaacggcagc accctggggc gcgagatgga taggaacgaa
     4981 acggcaggca gcaccggaaa gcccgggagc gcagccgaag tcttccgggt gttcctcggg
     5041 ctggggctga cctcattcgg tggcccagtc gcccacatcg gctacttccg ggagatgttc
     5101 gtcgttcggc gcaagtggct gagcgaaaat gcctttgcgg atctcgtcgc gctctgccag
     5161 ttccttccgg ggccggcatc tagccaggtt ggcatggcaa tcggccttct gcgggctggc
     5221 tatacggggc tgctcgcggc ctggctggca ttcaccctgc catcagccat tgtcctcgtc
     5281 gctttcgctt atggcgccgg aacgctcgcg caggccgcag gcacaggctg gatcagcgga
     5341 ttgaaggcgg ccgcggtcgc cgtggtcgcc aatgccgtgc tcggcatggc gcgcagcctt
     5401 gcaccggaca gggaacgggc gacgatcgcc gtcatcggca tgatcggcgc gatgctcatc
     5461 cccttcgcat tcgggcaggt attggtgatc gccattggcg gactgatagg gctggtctgg
     5521 ctcagcgcca aggttggacc tgtggaggag gacgcatcgc tgaccatcgc cgtcagccag
     5581 cgcgcggcaa ttctcttcct ggcgatcttc ttcgcactcc tggtgctgct cccgttcctc
     5641 gcgtcggtca gcgggaatgg cctgctgcgg ctcatcgatg ggttctatcg cgccggttcg
     5701 ctcgtcttcg gcggcggtca cgtcgtcctg ccgctgctcc aggcggaggt ggtcgagacc
     5761 ggcatggtcg gccgcgaggc attcctggca ggctatggcg cggctcaagc ggtgccaggg
     5821 cctttgttca cgttcgccgc ctatctcggc gcagtcatgc agcaagcccc gaatggtctc
     5881 accggtgcag gcatcgcgct tgtcgcgata ttcattccgt cggcgctgct cattctcggc
     5941 ggactgccgt tctgggaccg ccttcgcagt gcaccgctgg cacagcgagc ccttgccggc
     6001 gtgaacgccg cagtcgtcgg cctgctcgca gcggcccttt atgatccggt tttcgcagac
     6061 ggcatcacgt cgcacacggc catggcattg gccgctgcct cgttcgtggc gctggcggca
     6121 tggcaggttc ccgcatgggc ggtggtgatc gcagcgggcg tcgccggatt cgctgtcctc
     6181 tagatcggcg cccgccccgc catctgcggc tccatcatat cgcggaacac ccctttaccc
     6241 gcctttaacg caggccggcc tacaacgaac gggttgaagc gagtcattgg atggtactgc
     6301 gtcgcacaac accagatcgg cggatgagga ggacgcggca gcatatcctg gtactgcttg
     6361 gctgcctgtc gatgacggcc tacttcgtcc atcacacgat caccggcaag cacggcctcg
     6421 aggcgcgatc gcggctggtc gagcgctcca aagcactgga gcgggagatc gccggtctcg
     6481 acgccgtgca tgccgagctc atgcgcgacg tgctgctgct gcggcccgat ccgccccatc
     6541 ctgatctcat cgaagagatc gcgaccggcg tcctcggcat ggcccggccg ggtgacagga
     6601 tcgttctggt cggctccgac gttccttaac ggttggttca gcaaattggt ggcggactgc
     6661 ttgaccgctt ccctgccggc cgaatttctg gcaaaggcag gggcggatgt gtagtttggc
     6721 ggcggagcac acgttcgccc cgaattccag acgcttagga ggtcccatgg cacctgcgga
     6781 caaggcgcgg ggttcgaccg gcgccaggtc ggccaagacc gcggccagga acggctcggg
     6841 aaccgcagtt gcggagattt cgcctacggg tggggacgta gcggatttca cccgcgagga
     6901 ggaactgcac gcctaccgcg agatgcttct catccgccga ttcgaggaga aggctggcca
     6961 gctctacggc atgggcttca tcggcggctt ctgccacctg tacatcggcc aggaagcggt
     7021 cgtcgtcggc atgcagatgt gcgcgcgcga gggggatcag gtcatcacga cctatcgcga
     7081 ccacggccat atgctggcga ccggcatgga ccccaagggc gtgatggcag agctgacggg
     7141 ccgccgcgga ggctactcca agggcaaggg cggctcgatg cacatgttct ccgtcgagaa
     7201 gaacttttac ggagggcacg ggatcgtcgg cgcgaacgtg cctttgggcg cagggctcgc
     7261 gttcgccaac aagtatcgcg gcaacgacaa cgtcaccttc tgctacttcg gcgacggcgc
     7321 cgccaaccag ggccaggtct acgagacctt caacatgtcg aagctctgga agctgccgat
     7381 cctgttcatc atcgagaaca acaaatacgc gatgggcacg tcggtcgagc gctccgcggc
     7441 cacgaccgat ttctcccagc gcggcctgtc gttcgacatt cctggcgagc aggtcgatgg
     7501 gatggacgtg cgtgcggtga aagcggccgg cgaaaaggcc gtgaagtggg cacgcgaggg
     7561 caacggcccc tacattcttg aaatgctcac ctatcgctat cgcggccact ccatgtcgga
     7621 tccggccaag taccgcacgc gtgaggaggt cgagcgggtg cgtgaggaac gtgatcccat
     7681 cgagcgggtg cgggcgcgca tgctggagaa gaagtatgcg agtgaggaag agctaaaggc
     7741 gatcgacgcc gagatccgcg gtatcatcaa tgcagctgcc gaattcgcgc agaacgatcc
     7801 tgaacctgat ccgtctgagc tgtggaccga tgttttgaga taggccaatg agcgccgcca
     7861 aacctatttg cacctgccgc tgatgtctcc ctccccggtc gggggaggat gtgcagcgaa
     7921 tgttgagaga tcctgccgaa atgcccaccc aagtcctgat gcctgcactg agccccacca
     7981 tggaggaggg caagcttgcg aaatggctcg tcaaggaagg tgacgaggtc aagtccggca
     8041 tggtgattgc cgagatcgag acggacaagg cgaccatgga ggtcgaggcc gtcgacgaag
     8101 gccggctcga ccgcattctc gttcccgccg gcacggaagg cgtcaaggtg aacacgccga
     8161 tcgccgtcat cctgcaggac ggcgaggcgc ctggtacgcc ggtgccgggc tcgccgcaag
     8221 cgccgcgcga cttgaagcag gcggacgcgc aggccctgcc ggcacaggcc cgcccggctg
     8281 caccaccagg aggggagccc gtcgcacctc cgcaaccccc gcctgcgcgc ggtgtaggcg
     8341 aggaagagct tcctccggac acggaaatga tcacgcagac catgcgcgaa gccctgcggg
     8401 atgccatggc cgaggaaatg cgccgcgatc cggacgtttt ccttatgggc gaggaggtcg
     8461 cccagtacca gggcgcctac aaggtcagtc agggcctgct cgacgagttc ggcagccggc
     8521 gcgtgatcga tacgccgatt acggagcagg ggtttgccgg catcggcatc ggcgcggggt
     8581 tcgcgggcct gaagccgatc gtggagttca tgacctggaa cttcgccatg caggcgatcg
     8641 accagatcgt caactcggcg gcgaagacgc tttacatgtc gggcgggcag atgggctgct
     8701 ccatcgtgtt ccgcggaccc aacggcgcag cggctcgggt tgccgcccag cacagccagg
     8761 agttttcggc ctggtatgcg cacgtgcccg ggctgaaggt ggtggcgccg tcgaacccgt
     8821 ccgatgcgaa agggcttctc aagagcgcca tccgcgatcc aaacccgatc atcttcctgg
     8881 agaacgagat cctgtacggc gcctccggtc tggtcccgaa ggtggacgac tggctggtgc
     8941 cgatcggcaa ggcgcggatc gcacggccgg ggaatcacgt gacgatcgtc tcctggtcgc
     9001 gcggcatggt ctacgcgctc gatgcggcgg aacggctggc caaggaaggc atcgaggccg
     9061 aggtgatcga cctgcgcacc ctgcggccgc tcgatctcga aacggtgctg acctccgtcc
     9121 gcaagacgaa ccggatcgtg acggtcgagg aatcctggcc ggtgtgctcc gtgggctccg
     9181 aaatttgcgc ccaggttgct atccaagcat tcgactacct cgacgctcct cccgccaaga
     9241 tatccggcgc cgacgtgccg atgccttacg cggccaatct ggagcggctt gcgctgccta
     9301 acgcggagca ggtcgtggag gccgcaaagc gcgtgctgta cgtatagtgc cgtgggaaaa
     9361 cagccatact acgacgtcac gtcggggtga gagagcattg gcaccgtccg tgcataacta
     9421 tagcgggagc tgccactgcg gagccatatc cgtcgtgctg agcctttcgc ggccggcatc
     9481 cgagatgcgc gttcgggctt gccagtgcgg cttctgccgc aggcacggca cgcggaccat
     9541 cgccgatcca gcgggctcgg tggtgatctc gaccgcgtcg cctgatcaga tccgccgcta
     9601 tcgctttggg ctgaggacgg cggattatct tctctgcgcc acttgcggca gctacatcgg
     9661 cgcggtgcag gcggagcccg a
//
LOCUS       DASVDU010000072         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1210097, whole genome shotgun sequence.
ACCESSION   DASVDU010000072 DASVDU010000000
VERSION     DASVDU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210097"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..133
                     /locus_tag="VF226_04735"
     tRNA            58..133
                     /locus_tag="VF226_04735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:91..93,aa:Ala,seq:cgc)
     gene            624..1019
                     /locus_tag="VF226_04740"
     CDS             624..1019
                     /locus_tag="VF226_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073326.1"
                     /translation="MLKTTRALAASLAVATLFLASVNTVLAQEFRRLTSAEIREQIVG
                     KTLTGSGRAGNCTFFDRFAADGSATAKCGNYSDTGQWRLGDDALCIKWSKRPNEYCLT
                     FFTDGSKYQVINPNRGPTPFPISVVTGRE"
     gene            complement(1148..2272)
                     /locus_tag="VF226_04745"
     CDS             complement(1148..2272)
                     /locus_tag="VF226_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073327.1"
                     /translation="MADKIFLVRDGQSPVAVPSMAFEAEEVLQNLLVQAPDLLAGVTE
                     GGTSPRRWMLVKREKGIPDAEDGADRWSLDHLFLDQDAVPTLVEVKRASDERSRRKVV
                     AQMLDYAANAIRFWSISSIREAFAQTAEAEGQDPDQRLKALIGDADPEEFWKRVETNL
                     RDGRIRMIFVADEIAPELQRIIEFLNEQMRSATMAAVEIRHFRADGTRLLVPRVVGQT
                     ARAQAAKSAGASTTLPPISESEWLQKLAERSPEFPKIAQDLIAWFRTLPVETGLASKQ
                     AALYIRVINRGTWRYPFSIWSNGSLSVNFMWLLSSEPFADESARREVLNRLNVVPGLK
                     LTNQNIAGLPMISLSQFKDGTIMTGFKDVARWVVERLYAG"
BASE COUNT          496 a          828 c          838 g          516 t
ORIGIN      
        1 cgcttcgttc ttgactccgc gtcggccccc acccatattc cgccccaccg caccggcggg
       61 gccgtagctc agatgggaga gcgcctcgtt cgcaatgagg aggtcagggg ttcgattccc
      121 ctcggctcca ccattcccgc cgcaatcaag cacttatccg gactggccca gcggtcgcga
      181 cacgcgactg cgacgtttcg cgcgcgacac cggcgtcgtg agggaacagg gcggtttagc
      241 cgattccgcg cataggtcgc ctgtctgctc aggcatcagc gttatggccc cgcctctcgg
      301 ccgcggcgct ttggcgcagc acctcatggg gtggcatgcc gtctcccggg cccatccgaa
      361 aggcaagggc gacagccgga tcgacgaaca cgacgagggt tacggtcaac ccgatcgcgc
      421 gccagcaggc ggggcgagca tcgacagccg ctttgagacg gcgagatcag ccccggcgat
      481 cagtaagagc gcacctacag ccggaagggg gatcgggccg aataggtcgc acgcattcac
      541 atcaccgtag cgccgggcta tcctcacgct gccgcgctga acgtcacagc gctgaccata
      601 gaaaatagag aaaagggggg gatatgttga agacgacgcg tgcgttggct gcttcgctgg
      661 ccgtcgcgac actcttcttg gcgagcgtga acacagtttt ggcgcaagag ttcaggcgtc
      721 tcacctcggc cgagatacgc gagcagatcg tcggcaagac gctcacgggc tctggccgcg
      781 ccggcaactg cacgttcttc gaccgctttg ctgccgacgg ctcggccacg gccaaatgtg
      841 gcaactatag cgacaccgga caatggcggc ttggggacga cgcgctgtgc atcaaatggt
      901 ccaagcggcc caacgagtac tgcctgacct tctttaccga cggctcgaag tatcaggtca
      961 tcaatccgaa tcgtggaccg accccatttc ccatctcggt cgttaccgga cgggaataag
     1021 ggtaaggctg acggacaggc catgatctcg gacggtccgc gcacgtagga cgcggcggta
     1081 aggaccgagt ccgggagccc ggcgcgcaaa tgagccgcga tatgggattg atcccattac
     1141 gttgatctca tcctgcgtag agccgctcga cgacccagcg ggcgacgtcc ttgaagccgg
     1201 tcatgatcgt gccgtccttg aactggctga ggcttatcat tggtagccca gcgatgttct
     1261 ggttcgtgag cttgagacct ggtacgacgt tgaggcggtt caggacttcg cgacgtgcgc
     1321 tctcatcagc gaaaggttcg ctgctgagaa gccacatgaa attgaccgac agagagccgt
     1381 tgctccagat actgaacgga taccgccagg tgcctcgatt gatgacgcgg atgtagagtg
     1441 cagcctgttt gctcgcaagc cctgtttcca caggcagagt gcggaaccag gcgatcagat
     1501 cctgagcgat ctttggaaat tcgggggatc gttccgccag cttctgaagc cactcgctct
     1561 cgctgatcgg cggcaacgtt gtcgatgccc ccgcggactt ggccgcttgc gcccgtgccg
     1621 tttgtccgac cacccgcggg acgagcagac gggttccgtc cgcgcgaaaa tggcgtatct
     1681 cgacggccgc catcgtggcg gagcgcatct gctcgttcag gaactcgatg atgcgctgga
     1741 gttccggggc gatctcgtca gccacgaaga tcatacggat gcgcccatcg cgcagattgg
     1801 tctcgacccg cttccagaac tcttccgggt cggcatcgcc gatgagggct ttcagtcgct
     1861 gatccgggtc ctgtccctcg gcttcggcgg tctgggcgaa cgcctcgcgg atgctcgaga
     1921 tggaccaaaa gcgaatcgcg ttcgcggcgt aatccagcat ctgcgcgacg accttgcgcc
     1981 gggaccgctc gtcggaggca cgtttgactt cgaccagcgt gggcacggca tcctgatcga
     2041 ggaagaggtg gtcgagagac caccggtccg ccccgtcctc cgcgtcgggg atgcctttct
     2101 cacgcttcac cagcatccaa cgccggggcg aggtgcctcc ctccgtgaca ccagcgagga
     2161 gatcgggtgc ttgcacgagc aagttttgca gaacttcttc cgcctcgaac gccatgctgg
     2221 gcacggcgac cggggattgg ccatctcgaa ccaggaaaat cttgtcggcc atgggcgcgg
     2281 taccaacgca gcgccactac cttggatagt gttaccttac gcgtcaacta gaatgagttt
     2341 tggatctcaa ggattggtac ctatgcgagc atgatcctgc tggattccag aatcgcgacc
     2401 tgaactttct ggaacttcac aggtcgggcc ttcggacgat gcgatgcatc gcggcttcgg
     2461 ggcggcgctc gacggttcca acctggcgcc tcgtgttttc ccgcatgacg tggttcccat
     2521 atggcgtggg ttcgatgctg ccaagccggc ggaaccgggc cgatgccgcg tgggttattg
     2581 ttattcatca ttgagtgccc gagggcccac acgggcgatg gaccgttgcg gtcccttgaa
     2641 tggcgaagcg aggtattccc atctctcgcc ggccgccc
//
LOCUS       DASVDU010000073         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1162483, whole genome shotgun sequence.
ACCESSION   DASVDU010000073 DASVDU010000000
VERSION     DASVDU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162483"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VF226_04750"
     CDS             <1..1101
                     /locus_tag="VF226_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX7073328.1"
                     /translation="TREFNRGTWTIGYTGQSPERLKLHMQNQYTFDPTTLRANGGPCD
                     GDYYGLPWPCWGTPEIKHPGTPILYDTSKPVAEGGLCFRARFGVERDGQNLLAEGSYP
                     VGSEIQDGYPEFTMAMLQKLGWDKDLTAEEREVIARIGGNNIGAVNWKTDLSGGIQRV
                     AIAHGCAPFGNAKARAVVWNFPDPVPIHREPLYTPRRDLVAQYPTYEDTNAWRVPTLY
                     RSIQEKDFSKDFPIILSSGRLVEYEGGGDETRSNPWLAELQQNMFVEINPYDANQAGV
                     RDGEMVWVYGPEGGKVRVMALVTERVGRGVAWMPFHFGGWFQGQDLRSKYPEGADPYV
                     LGESTNTAQTYGYDSVTQMQESKTTLCRIERA"
     gene            1113..1709
                     /gene="fdh3B"
                     /locus_tag="VF226_04755"
     CDS             1113..1709
                     /gene="fdh3B"
                     /locus_tag="VF226_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEX7073329.1"
                     /translation="MARMKFLVDAERCIECNACVTACKNENHVPWGINRRRVVTINDG
                     KPGERSISVACMHCSDAPCIAVCPVDCIYNTEEGVVLHSKDLCIGCGYCFFACPFGAP
                     QYPQAGNFGSRGKMDKCTFCAGGPEEANSPAEFQKYGRNRLAEGKLPLCAEMCATKAL
                     LAGDGDVVSNIYRERVVARGFGSGAWGWGTAYQLKAGG"
     gene            1843..2052
                     /locus_tag="VF226_04760"
     CDS             1843..2052
                     /locus_tag="VF226_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073330.1"
                     /translation="MKRVKAVGTRGLVIGACCLVVGLALQACRDEEQGRILYFEKGTY
                     LGKADQPLTEDQLRALRQRTSNQQM"
     gene            2070..>3017
                     /locus_tag="VF226_04765"
     CDS             2070..>3017
                     /locus_tag="VF226_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEX7073331.1"
                     /translation="MRRNRRTLAGFGAAMVFALACLMIVALPASPDLGHPAMAQQEGH
                     VPGGTLGNTSDAEFWRAIRQGEQGYVSIPDKRAATLIQSEGENWRAIRNGPLSTYGIW
                     ALLGIIALLALFFALRGRIRIEHGRSGRTVERFNGLERFGHWLTATSFIVLALTGLNM
                     LYGRYVLKPILGPEIFSAVTLGGKYAHNFLAFAFMVGLVLIFALWIRDNIPDRNDVKW
                     IAKGGGLFTKGTHPPARRFNAGQKIMFWLVILGGASVSLSGIALLFPFEFSLFSKTFA
                     FLNLFGFDLPTNLAPIQEMQLSQIWHGIVGLLLIAVIIAH"
BASE COUNT          524 a          948 c         1015 g          530 t
ORIGIN      
        1 acgcgcgagt tcaatcgcgg cacctggacg atcggctaca cgggccagtc gccggagcgg
       61 ctcaagctgc acatgcagaa ccagtacacc ttcgacccga cgacgctgcg ggccaatggt
      121 ggaccctgcg acggcgatta ctatgggttg ccctggccgt gctgggggac gccggagatc
      181 aagcatcccg gcacgccgat cctctatgac acctcgaagc cggtggcgga gggcggcctc
      241 tgcttccgcg cccgtttcgg cgtcgagcgg gacggtcaga atctgctcgc cgagggctcc
      301 tacccggtcg gctcggagat tcaggacgga tatcccgagt tcaccatggc catgctccag
      361 aagctcggct gggacaagga tctcacggcc gaggagaggg aggtcatcgc caggatcggc
      421 ggcaacaaca tcggcgccgt gaactggaag accgatctct cgggcggaat ccagcgggtg
      481 gcgatcgcgc atggctgtgc gcccttcggc aatgccaagg cccgcgcggt cgtctggaac
      541 ttccccgatc cggtgcccat acaccgcgag ccgctctaca cgccgcggcg cgatctggtg
      601 gcacagtatc cgacctacga agacaccaac gcgtggcggg tgccgaccct ctaccgctcg
      661 atccaggaga aggacttctc gaaggacttc ccgatcatcc tgtcgtcggg acgtctggtc
      721 gagtacgagg gcggcggcga cgagacccga tccaacccgt ggctcgcgga gctccagcag
      781 aacatgttcg tcgagatcaa cccgtacgac gccaaccagg ccggagtccg ggacggcgag
      841 atggtctggg tctacgggcc ggaaggcggc aaggtcaggg tgatggccct ggtcaccgaa
      901 cgggtgggac gcggggtcgc ctggatgccg ttccatttcg gcggctggtt ccaggggcaa
      961 gacctgcgga gcaagtaccc ggagggggcc gacccctacg tgctgggtga atcgaccaac
     1021 accgcgcaga cctatgggta cgactcggtc acccagatgc aggagagcaa gaccaccttg
     1081 tgccggatcg agagagcgta gggagcgaag ccatggcacg gatgaaattc cttgtcgacg
     1141 ccgagcgctg catcgagtgc aacgcctgcg tcacggcgtg caagaacgag aaccatgtgc
     1201 cctggggcat caaccggcgc cgggtcgtga cgatcaatga cggcaagccc ggcgagcggt
     1261 cgatctcggt cgcctgcatg cactgctccg acgcgccctg catcgcggtc tgcccggtcg
     1321 attgcatcta caacacggaa gagggcgtgg tcctgcattc gaaggacctg tgcatcggct
     1381 gcggctactg cttcttcgcc tgcccgttcg gcgcgccgca atatccgcag gccggcaatt
     1441 tcggaagccg gggcaagatg gacaagtgca ccttctgcgc cggcggcccg gaggaggcga
     1501 acagccccgc cgagttccag aagtacggcc gcaaccgtct cgccgaaggc aagctccccc
     1561 tctgtgcgga gatgtgcgca accaaggcgc tcctcgccgg cgacggcgac gtggtctcga
     1621 acatctatcg cgaacgcgtt gtcgcccgcg gcttcggttc gggcgcctgg ggctggggca
     1681 ccgcttacca gctgaaggcc ggcggctgag ggaaggttgc aaccgcagag gaccgcgggg
     1741 ggcgacgacg ggtcgcccgc cgcggcttcg gccgtttgaa gcagagagca tgagtgtgcg
     1801 gacgaggcat cggtgttgcc ttcgtcggcc ttgggcaggg gcatgaagcg ggtaaaggcg
     1861 gtcggaacga gagggttggt gattggcgcc tgttgcctcg tcgtgggctt ggccttgcag
     1921 gcctgtcggg acgaggagca ggggcggatc ctgtatttcg agaaggggac ctatctcggc
     1981 aaggccgacc agccgctgac cgaggatcag cttcgcgccc ttcgccagcg cacctccaac
     2041 cagcaaatgt aagcacggga aggttttcga tgcggcggaa ccgacgaacc ctggccggct
     2101 ttggcgcggc gatggtgttt gcgctggcct gcctgatgat cgtggcgctt ccggcgtcgc
     2161 cggatctcgg ccatccggcc atggcccagc aggaggggca cgtaccggga ggtacgctcg
     2221 gcaacaccag cgacgccgag ttctggcggg ccattcgcca gggcgagcag ggctacgtct
     2281 cgatccccga taagcgggcc gccaccctca ttcagtcgga aggcgagaat tggcgggcca
     2341 tccgcaacgg tcccctctcg acctacggca tctgggcgct gctcggcatc atcgccctgc
     2401 tggcgctgtt tttcgccttg cggggccgga tccggatcga gcatgggcgc agcgggcgca
     2461 cggtcgagcg cttcaatggg ctcgaacgct ttggccattg gctgaccgca acgtcgttca
     2521 tcgttctcgc cctcaccggg ctcaacatgc tctatgggcg ctatgtcctg aagccgatcc
     2581 tcggaccgga gatcttctcg gccgtgacct tgggcggcaa gtacgcgcac aactttctgg
     2641 ccttcgcctt catggtgggg ctggtgctga tcttcgcgtt gtggatcagg gacaacatcc
     2701 ccgatcgaaa cgacgtcaag tggatcgcca agggcggcgg gttgttcacg aagggcacgc
     2761 atcctccggc gagacggttc aacgccggcc agaagatcat gttctggctg gtcattcttg
     2821 gcggtgcctc cgtcagcctc tccggcatcg cgctgttgtt cccgttcgag ttctctctgt
     2881 tctcgaagac cttcgcgttc ctgaatctct ttggattcga cctgcccacg aatctcgcgc
     2941 cgatccagga gatgcagctc tcgcagatct ggcacgggat cgtcgggttg cttctgatcg
     3001 cggtgatcat cgcccat
//
LOCUS       DASVDU010000074        17288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_436719, whole genome shotgun sequence.
ACCESSION   DASVDU010000074 DASVDU010000000
VERSION     DASVDU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17288
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436719"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF226_04770"
     CDS             complement(<1..187)
                     /locus_tag="VF226_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7073332.1"
                     /translation="MYEIDPSRYTRHDSLIDAAAQRHRVSQALIKAVIMAESGFNAAA
                     YREEPQIKDASRGLMQLL"
     gene            complement(195..419)
                     /locus_tag="VF226_04775"
     CDS             complement(195..419)
                     /locus_tag="VF226_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073333.1"
                     /translation="MGEDRIDWLAARLDRLETNLGGRIDCLQETLEERLRKVEGAQAT
                     MQGAIGALKWLFGTLVGIGGLVVAIIALGG"
     gene            complement(523..3246)
                     /locus_tag="VF226_04780"
     CDS             complement(523..3246)
                     /locus_tag="VF226_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073334.1"
                     /translation="MASDPLSTRLLSGLSGPLEAGGNLLGAGLRGFENVADIAARPDF
                     ALFGAVRDILRLQNPVRGFIRGLAPRSDEDRANTFTLLRTVVPRGSFEEMALGTVLGL
                     SPRAIDTAFRIAGFVGDIALSPTDAVTRLIRGARLVPRGIAAGTRAAEQAIRALSASD
                     LGRRAVEAQTRLGELISGPARTGKEILRQHLSTRIRLPDGENIPVNVTAAEQEQAIRE
                     ALIRETGLSVAERQRMAQRRMERVLEIPESLRSAEAALNVTVQNARSFLNFRRAQVIE
                     QGSRLERRVQRVEKRFGVSRDRIFDLVEESGRDPAAIAQQPAEVQALVRDIRTLNKER
                     LAREVAAGVPVRELGDILEYMAHLLTPEAKDAIQRQANFRPQSFAEWTVRNRHQLERQ
                     FRGKSVRQINELVRTQGLEIDGRLIRVDKFFEDDPVKVLVARGLAGERAIANAQFLDD
                     MARLYGLPPSDPRAADWVSLDDIPQFGNLMKGLRFHPVIAREIVRIVERASPNAADQF
                     AGLLRRYTQQWKVGTLAFRPAYHFRNVMGNLWNNWLAGVSDPQVYADAVRVLRDDAAT
                     VRIAGFDMPAAEIRRAAEERGVVRQGFYSIETQREFAQFANTSKRSIPAGTAFRLQQM
                     YEDWSRLAHFIDRLKKGDTVDEAALSVKRYLFDYSDLTDTEKNILRPVFPFYSWWRKN
                     IPLQLEKLIEEPHKFAVLEQARLAFERDRQTVPEPITPEFLRGVRGIEVAPGAFVRLA
                     GNIPSAELAELSQPLRFLVSATHPLIQAPLQLVFGRTAFSGREISPTRIRPGLEPLTE
                     TEASLLDVVPGGVAVAEAFRVLREPTPVRAALRAIGVPIFEVDPGRQRGILRSQLMEE
                     RRRQLRAIQRAETEAERQRRRRILEDINRRLREVLGAGMTTPQSFIGSPSQ"
     gene            complement(3247..3729)
                     /locus_tag="VF226_04785"
     CDS             complement(3247..3729)
                     /locus_tag="VF226_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073335.1"
                     /translation="MANGRPQTLLEGFRQTFRAPFGSAGLGQFLPQRLPVTQLLQQAG
                     INVPAPFQGLSAEQIADIAQAARALEPFQPARSPLQNPGELVDFVISLWRSDNQLRFN
                     AGQPQRAFFQFLEEEGFRPDIRQMFTVLFDPQRLARQQQQQQNRPNLFERILGIGGER
                     "
     gene            complement(3742..4161)
                     /locus_tag="VF226_04790"
     CDS             complement(3742..4161)
                     /locus_tag="VF226_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073336.1"
                     /translation="MAMNAPTTARQGFRQVFGSTGLGALLPQRPSVTDILQQLGVSVP
                     QALQGLGADTLQDVLGIQRQLTQTSPTFQTFFQDPAEAARVVLSIWREFNRLVRDEGG
                     DPVGLLQFVQEAGFSPQIQQMFATLFAGGGTSLAPGA"
     gene            complement(4177..4881)
                     /locus_tag="VF226_04795"
     CDS             complement(4177..4881)
                     /locus_tag="VF226_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073337.1"
                     /translation="MASVIPAALSAIGQGVVAAAPAVIGQALGAVASNAFQRSGPQPP
                     PVTQALSPQANTLINELARRSIQRLRTADDLAGSTVGQAFGVARERVTEAADEAQRRL
                     QDILNARGVLRSGLLAENAREIEERRLQSLRDLAAQAAQARLNVGSQIQREALSALPA
                     IALPGQLAFQQAQQQFARRRGEFEAFRPAISTGLEAIVGGFESGLSEFLRRRRLSSIR
                     GGAPIHSGGGTVRFTF"
     gene            complement(4886..5353)
                     /locus_tag="VF226_04800"
     CDS             complement(4886..5353)
                     /locus_tag="VF226_04800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7073338.1"
                     /translation="MHLTPLKPEELPILRRWMEDPALFRLVHLEPVDLSRPWFVWMIR
                     TEDGRPVGWIDLFHVDWQNLRAEVGIGIPDPRGKGLSLFAARALRRIVFDEWMLHRVT
                     ARVRASNEPVIRLLKAVGFEQEGTERQALRTRDGWEDLLIFGILNPKSDDESG"
     gene            complement(5328..5519)
                     /locus_tag="VF226_04805"
     CDS             complement(5328..5519)
                     /locus_tag="VF226_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073339.1"
                     /translation="MESRDWLQQKLMEVSSEEVRLKGLLYDIEQNLKALRALKAYLEQ
                     SARTGAAQGVPDAPDAAEA"
     gene            complement(5534..6289)
                     /locus_tag="VF226_04810"
     CDS             complement(5534..6289)
                     /locus_tag="VF226_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073340.1"
                     /translation="MAQVRKPTAFAAADVPSGSHLNNNFDTLYNEFNGNIDSANLKSD
                     RLSMAKVSGGFWNVDASGNLIPDTGFSRWIGSPSLPVAISHEGVLKLVGTTSGGVTTH
                     QPFIQWLKDGVRRWEMGLDVANSVIEDFYIARDIGAGVSDIVYLREYLEGGNRRVAIG
                     LNFTPPPSGARVSISGADNSAWHDLLLRVAFGQTGDALKVTDSGGASIFDIDATGKIG
                     YAASNTTGTAGAQEGFLLVKVAGTVKKVPYHAA"
     gene            complement(6304..7056)
                     /locus_tag="VF226_04815"
     CDS             complement(6304..7056)
                     /locus_tag="VF226_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073341.1"
                     /translation="MAQINKPYNFIEDTVIDPTQVNANFDTIYNEFNGSISAANLASD
                     RTAFNKVTGGFWSIDASGNLSGSGDILPVNDLTQALGDATHRWDVYADGINVNTLTTD
                     LKPDLNQTRNLGSSSLRWLKVWAKDIDLTGDLLPAVNAVQALGSDTLRWDVFADGVNT
                     TSLLLGTGGVTSDFFPDATASQRKNGTSSNRWAEVWAERHPFVPISEPAAPATDNVVL
                     FVTATGTSPNREVALKAKFHTGAVQILASITY"
     gene            complement(7058..8539)
                     /locus_tag="VF226_04820"
     CDS             complement(7058..8539)
                     /locus_tag="VF226_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073342.1"
                     /translation="MARPVLLNVRDLSGGVNRTIHPHLLKDNEVINCRNMVFRTGVPE
                     KRGGSQRYNAVSLGSDPVKGIVRFYRSDGTKRLVIGVGTTLKVGDDVNGTFSDIATGL
                     TANREWHFEQWKDLLFATNAVDPLKQYDGTTVTDVAGSPPRAQFIVQHQNRLFLAGDP
                     ANPSRLYFSELDDHQAWPASNFIDIETSSGAGDWITGLASSFGDLVIFKHRSTHLLIG
                     DRQENWVRREIFPGIGCVAPRSVTTDGIWTYFLGADGIYRMRGTTLEHLSHKVNLDLL
                     RSGNLSVAAAAVHDNFLWLTTPIESSVDNSEALTFVFDLETKTWTIFSGIRAHAYAVW
                     DGAGDGHEITWGDASQGLVFFGDRGTSDDGQPIDAHFTSKAYDLDSFTVIKAFRRFWI
                     DVHSSGAKWTVSYQIDQSGPGATMDMSSAIIGASGATFWGRAIWGVSRWSTSRIQTFS
                     ESFRREDFGASITVTIRNNSSTDSGSIHGFAVEAIPRRVLRRA"
     gene            complement(8543..9166)
                     /locus_tag="VF226_04825"
     CDS             complement(8543..9166)
                     /locus_tag="VF226_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073343.1"
                     /translation="MTRDELTDAVRKLYGEASTTHTDDLTIHNWLNRAIEELLGDAGA
                     EEKLATFMSVPGQQEYGGADVGFPIDFMRPLAVYYDGLKLRHIDFREIVDASPDLQGT
                     PLGYYLRLNPVKLGFHPIPADAKEIRLFYQASPTLMAAGTDEPAIPAPYHYALAYWAA
                     AQWALADENIQRASAFMLQFERRKLELLGAGMSRTAETYPTVRWTGD"
     gene            complement(9171..9545)
                     /locus_tag="VF226_04830"
     CDS             complement(9171..9545)
                     /locus_tag="VF226_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073344.1"
                     /translation="MPVGDVYDIERRVREVYPDLRIFGEPGCWLVKMVKPVLVQLKSG
                     LAYATDRLEHVLTWKKPVLDARLLHHLRYTDQWSGHTDVLEELEEEEYLRRRAEERAA
                     DEVAAAFAKEARKALAMDFGWR"
     gene            complement(9669..9911)
                     /locus_tag="VF226_04835"
     CDS             complement(9669..9911)
                     /locus_tag="VF226_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073345.1"
                     /translation="MTAAEWLDQRFGTDDTFSPMSSYTASRQFFLDVFGPTFEAAYNA
                     GKRGQELHDALLQTSGGTRGWELYFADWLRRGLFDD"
     gene            complement(9911..10717)
                     /locus_tag="VF226_04840"
     CDS             complement(9911..10717)
                     /locus_tag="VF226_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073346.1"
                     /translation="MAVTFIDEASQLPVDRVSLFGRTVQPEVVTITTTPLAAGGIRSS
                     GVIDLMASPGIAMLAYIINGDTVTQFDWIPGDGTAGENPAISRARVTVPKALGAQDVQ
                     FIPPFRPIGRNYVVTVRNQTPTPQTRLRVFELRYRALEQITPQNLRGVLAQDLAIRDT
                     ATHSKFTDPQFVFDLVRTVWGGGKVPIYVDNQLDQEVTVTVRLQMLNVITASLGTIAV
                     PAGSKKVITNQDFPGLDVPSEVIDLLFTCSVAPTSGVVNAYIDTLVGGSA"
     gene            complement(10781..11986)
                     /locus_tag="VF226_04845"
     CDS             complement(10781..11986)
                     /locus_tag="VF226_04845"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX7073347.1"
                     /translation="MAQNLTNFDAVLKDFYLGPIRRQLNDRVIFLRRMERRTDVFSGR
                     QIVTPLSFSRNAGVGSRSDGGTLPTPGNQQYRDLILKPKYIYGSIKITGPTIAASRNN
                     AGAFVRAVRAEMDGMMRDLRRDVNRQFYNDSFGTIAGCASNTSTTTLQLDADANMNLF
                     YEGMKIDIKNSSGADITGGGDRTVVSIDRANKTITIDSAGGAVTTADTDAIFLSDSRG
                     LEIEGLEAWVGSETNTVGGIDRSDAANGWFRPNVLDNGGTLRSLSLDLLQQANDEAEA
                     RAGGRISIGITSHGVRRKYVALVRADRRHVNTMELDGGFTGVEFNGYPLIVDSQAPLN
                     TVWLLDESTFFIAQMADFDWLDEDGQILHRIVGSNASEDAYEATLAFYGNLVNTVPAA
                     NAALKDITE"
     gene            complement(12002..12826)
                     /locus_tag="VF226_04850"
     CDS             complement(12002..12826)
                     /locus_tag="VF226_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073348.1"
                     /translation="MPDDVQTFEQAGEAAEQALQQAFADEEAESSASEAEGDKPQPEQ
                     EPRRYKIRHRDREHEVTEEELINLAEQGFDYQQKRQRDAQALREYEQFKPLVDYIRSD
                     PQFAAYLAAYDQLRARGFTAAQAQAGAAQQTGLNLPPELIQDYTQLKQQVAEIKRAEE
                     ERETARIERQMREELERARKEFEGVKITENRIVDRIEHLLDDPVMQVRAAVLLEHFDD
                     IRNLVIQQYLNSKTRGGPPVQGTRGASPANRHQPPQTMKEADQAAIRFLEQMARQQ"
     gene            complement(12948..14993)
                     /locus_tag="VF226_04855"
     CDS             complement(12948..14993)
                     /locus_tag="VF226_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073349.1"
                     /translation="MAIMTPGVKGSSPIALVEERYEIGKEHRRPYEEQWRMNLAFYVG
                     KQWHIVHPATGKLIEPPSPPWRVRMVANYLLSFVWALFAKLTKNRPVMVAKPATGEED
                     DINAARRASKLLDYLWRHLELDDVRNLALLWMLTCGKAFMKTYWDGQAGPQIDEFIGD
                     RAITFFVGEVAVEAASPFEVVVDPAADSTGTLKGAEWMIHEKIRTVESIEEQYGVRVP
                     PEDVSKADFADFVASGFTRGFDVEDRTSSSKKLDHHAIVKEYWERPSPRFPNGRMIAV
                     ASGKLLVDGPFPMTQRRNSDPNVPFHPDRRFPFVELQHIIVPGQFWPMSLVEGCISLQ
                     RQINRVISKLQEQALILSSGKILSPWGAIDEKRITDEPGQVVSYRGAVPPTFWTPPPL
                     PQSLFNLISVYLETMRDIMGVRETSLGAPPPGVRSGVALSLLLEQDETKLGPVVANVE
                     KAFSGIGSRILCLAQQNYLEPRTVEIAGRNGIAETIDFYAADIRSTDVEVQAGSALPK
                     LKSAQQQFIVSLAESGFIDIRDPEERRRALKLMDAGIFEPEVDEETLDEQRAELENKR
                     MAAGIGQLPNHWENHQVHIREHNRFRKTDDFERLDPLTKQLFEIHVRQHEMLAMQLAQ
                     QDLQRAAVIQGAVQQPGAVSLPPEAQAGGVPAVSPAPPGLGGGSSPPVGPPGGPPPA"
     gene            complement(15008..15253)
                     /locus_tag="VF226_04860"
     CDS             complement(15008..15253)
                     /locus_tag="VF226_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073350.1"
                     /translation="MPDAYIVLAIVVAWAAWREWLQLSERRMWDAERTALLNRLMSRS
                     FGEYVQAQIAAGHSSRAEEEDAEQSALRQLAERYYNS"
     gene            complement(15246..15605)
                     /locus_tag="VF226_04865"
     CDS             complement(15246..15605)
                     /locus_tag="VF226_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073351.1"
                     /translation="MRLIDAPDMHPGCCLICRCTPQGPFVDCETEVEEEWVFICSGCV
                     SEIVATAAQDDRLLNLVLDRLRSLRPSLLRGDRPSADTATVECPQCGRVYTGRLAGHN
                     LRRHMESVHAEEVAVGA"
     gene            complement(15602..15802)
                     /locus_tag="VF226_04870"
     CDS             complement(15602..15802)
                     /locus_tag="VF226_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073352.1"
                     /translation="MAEERKPPLGTGERFRALVRKLRRRGNVRDPEALAAHIGRQEHG
                     ASRMAELAARGRRRRMRQEGAS"
     gene            complement(15814..17157)
                     /locus_tag="VF226_04875"
     CDS             complement(15814..17157)
                     /locus_tag="VF226_04875"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEX7073353.1"
                     /translation="MTVQTVAIDYTPLPRQAEFHRSPARYRAYIGGFGSGKTRCGCME
                     ALVTALEHPGSLGVIARQTYPELRDTTQRTFFEECLPQELLRLSTWRRSEGHLILPNG
                     SEVLFRYLENADETLKSLNLSWFYIDEASEVAETVFLMLQSRLRNPIGPRRGWITSNP
                     AGHDWMYRRFVSREAIPPEGRQDYALIHAPTRENVHLPPGYVESLLSSYPPEWVRKYL
                     DASFDVFEGQIFTEFDESKHVIDPLPDGIPEGWEKLAGFDYGLRNPTAVVWAAVDWDG
                     NVIIYDEHYQAEWPPEMHAKEILRRGKVPIYADPSVFNRGPTGEAVGNIYHEQGVTLI
                     PAQNDVDAGINRIHEWLSGDPPRLRIYRTCSNLIRELKGYRWAPRRTAVGDEPERPLK
                     QDDHAVDALRYLLMARPQPTQRPEEPKPNTATTRATRHIERLVAARNRTNDPGYW"
BASE COUNT         2928 a         5775 c         5389 g         3196 t
ORIGIN      
        1 gaagcagttg catcagcccg cggctggcgt ccttgatctg cggctcctcg cgataggccg
       61 cggcgttgaa gccggactcc gccatgatga cggccttgat cagcgcttgg ctcacccggt
      121 gccgctgtgc cgcggcatcg atcagggagt cgtggcgcgt gtagcgggag gggtcgatct
      181 cgtacaccac cgtctcagcc ccccagcgcg atgatggcga ccaccagccc gccgatgccc
      241 accagcgtac cgaacagcca cttcagcgcc ccgatggcgc cctgcatggt cgcctgggcg
      301 ccctccacct tgcgcagccg ctcctccaac gtctcttgca ggcagtcaat ccgcccgccg
      361 agattcgtct ccaggcggtc cagacgggct gccagccaat cgatacggtc ttcccccaaa
      421 cccgaccacc cccgaggaag gaatgtctgc acacgcgccg aacgcgcaac agccgtcgcc
      481 gctgggctcc aatccgcggg cgacggcccg ggggctctgc ctttactgcg acggcgaccc
      541 gatgaaggac tgcggcgtcg tcattcccgc gccgagcact tctcgcagcc gccgattgat
      601 gtcttccagg atgcggcgcc gccgttgccg ctccgcctcc gtctcggccc gctggatcgc
      661 cctgagctgg cgacgtctct cctccatcag ctgagaacgg aggatccccc gctggcgacc
      721 gggatccacc tcgaagatcg gcacaccgat ggcccgcagc gcagcccgaa ccggtgtcgg
      781 ctctctgagc acccggaagg cttcggctac cgcaacaccc cccggaacca cgtccagaag
      841 ggaagcctct gtctccgtca gcggctccag gccaggacgg atgcgagtcg gcgaaatctc
      901 gcggccggag aacgcggtgc gtccgaagac gagctgcaaa ggcgcttgaa tcagcggatg
      961 agtcgccgag acgaggaacc gaagcggctg agacagctcc gccagctcgg cggaggggat
     1021 gttccccgcc aggcggacga atgcgcccgg cgccacctcg atgccgcgaa cgccgcgcag
     1081 gaattcggga gtgatcggct cgggcaccgt ctggcgatcg cgctcgaaag cgagcctcgc
     1141 ctgctcaagg accgcgaact tgtggggctc ctcgatcagc ttctcgagct gaagtgggat
     1201 gttctttcgc caccagctgt agaatgggaa tacaggccgc aggatgttct tctccgtgtc
     1261 ggtcaggtcg ctgtagtcga agagataccg tttcaccgac agcgcggctt catcgaccgt
     1321 gtcgcccttc ttcagccggt caatgaaatg cgccaacctc gaccaatcct catacatctg
     1381 ctgcaaccgg aaggctgtgc cggcaggaat cgaccgcttc gacgtgttgg cgaattgggc
     1441 aaactcccgc tgcgtctcga tcgagtagaa tccctggcgg acgacaccgc gttcctcggc
     1501 ggcgcggcga atctcggctg ccggcatgtc aaagccggcg atccgcaccg tcgccgcatc
     1561 gtcccgcagc acccgcaccg cgtcggcata aacctgcggg tcggacaccc cggccagcca
     1621 attgttccag agattcccca tcacgttgcg gaagtggtac gcaggccgga atgcaagcgt
     1681 tcccaccttc cactgctgtg tgtatcgcct gagcaggccg gcgaactggt cggccgcgtt
     1741 cggtgatgcg cgctcaacga tgcgaacgat ctctcgggcg atcacgggat ggaatcgcag
     1801 ccccttcatc aggttgccga actgcgggat gtcatcaagg ctcacccagt ccgcggcgcg
     1861 cggatcgctg ggcggtaggc cgtacagccg cgccatgtcg tcgaggaact gggcgttggc
     1921 gatcgcccgc tcgccggcaa gcccgcgggc gaccagcacc ttgaccggat cgtcctcgaa
     1981 gaacttgtcc acccggatga ggcgaccgtc tatctcaagc ccctgggttc gcaccagctc
     2041 gttgatctgc cgcaccgatt tgccgcggaa ctgcctttcg agctgatgac ggttgcgtac
     2101 cgtccactcg gcgaagcttt ggggtcggaa gttggcttgg cgctggatag cgtcctttgc
     2161 ttcgggggtc agcagatgcg ccatatattc gaggatgtcg ccaagctcgc gcaccgggac
     2221 gccggcggcc acctcacggg caagacgctc tttgttcagc gttctgatgt cccgcaccag
     2281 cgcctgcacc tcggcgggct gttgagcgat agccgcagga tcgcggccgg actcctccac
     2341 gaggtcgaag atgcggtccc gggagacgcc gaatcgtttc tcaacacgct gcacgcgccg
     2401 ctccagacgg ctcccttgct caatcacctg cgcccggcgg aagttgagaa aagagcgggc
     2461 gttctgcacc gtcacgttca gcgcggcctc ggcgctccgt aggctctcgg gaatctccag
     2521 gacccgctcc atacgtcgct gcgccatcct ttgcctttcg gccaccgaca gccccgtctc
     2581 ccggatcagc gcctcacgaa tggcctgctc ctgctcggcc gccgtgacgt tcaccgggat
     2641 gttctcgcca tcaggcagcc ggatgcgggt gctgaggtgc tgccggagga tttccttgcc
     2701 cgtgcgggcc ggaccggaga tcagctcgcc gagcctcgtc tgcgcctcga cggctcgccg
     2761 ccccagatcg ctcgcgctca gcgcgcggat tgcctgctcg gcagcccgtg tgccggcagc
     2821 gatgcctcgt ggaacaagac gagcgccgcg aatcagacga gtcacagcgt cggtggggct
     2881 cagagcgatg tctccgacga agccggcgat gcgaaaggcg gtgtcgatcg ccctcggcga
     2941 cagaccgagc accgtgccga gcgccatctc ctcgaacgag ccccgcggaa cgacggtccg
     3001 aaggagagtg aacgtgttgg cccgatcctc gtcggagcgc ggggcaagcc cgcggatgaa
     3061 tcctcgcacg ggattctgga gccggaggat gtccctgacc gcgccaaaca gggcaaagtc
     3121 gggccgggcg gcgatgtcgg cgacgttctc gaagcccctc agccccgcgc cgagcaaatt
     3181 cccgcccgcc tccagcggac ccgaaagacc cgagagaagc cgggtgctca aaggatcgct
     3241 tgccatctac ctctctcctc cgatgccgag aatgcgctcg aacagattcg gtcggttttg
     3301 ctgctgctgt tgctgccggg caagccgctg cgggtcgaac aacacggtga acatctgccg
     3361 gatgtctggg cggaagcctt cctcttctag gaattggaag aatgcccgtt gcggctggcc
     3421 ggcgttgaaa cgaagctgat tgtcactgcg ccagagagag atgacgaaat cgaccagctc
     3481 tcccgggttt tgcaggggcg agcgcgccgg ctggaagggc tccagcgccc gagctgcctg
     3541 ggcgatgtca gcaatctgct ccgcgctcag tccttggaag ggggccggta cgttgatgcc
     3601 cgcctgttgc aggagctggg tgacggggag ccgctgagga aggaactgcc cgagcccggc
     3661 cgagccgaac ggtgcccgga acgtctgccg gaatccttcc agcagggttt gaggccgacc
     3721 gttcgccatc aggacacctc cttaggcacc gggagcaagg cttgtgccgc ctccggcgaa
     3781 gagcgtcgcg aacatctgct ggatttgggg agagaacccg gcctcctgaa cgaactggag
     3841 gagacccacc ggatcgccgc cctcgtcccg caccagccgg ttgaactccc gccagatgga
     3901 gagcaccacc cgcgccgcct ccgccgggtc ctggaagaac gtctggaacg tgggagatgt
     3961 ctgagtaagc tgccgctgga tgccgagcac gtcctgcaag gtgtccgctc ccagtccttg
     4021 cagggcttgc ggcacgctca ctccgagctg ttgcaggatg tcggtcacac tgggccgctg
     4081 cggaagcagc gctccaaggc cggtggaacc gaaaacctgc cggaatccct gccgggccgt
     4141 cgtcggtgca ttcatcgcca ttgcgatcat cctcccttag aaggtgaatc tgactgtacc
     4201 gccaccactg tggatcgggg cgcctccgcg gatcgaagac agtcttctgc gtctgagaaa
     4261 ctccgatagg ccgctctcga agccgccaac gatcgcctcc agcccggtcg agatggctgg
     4321 acggaacgcc tcgaactcgc ctcgccggcg cgcgaactgc tgctgtgcct gctggaaggc
     4381 gagctgcccc ggcaaggcga tggccggaag ggccgagagc gcctcccgct ggatttggga
     4441 gccgacgttc agcctcgcct gcgccgcctg ggcagcgaga tcgcgcaggc tctgcaggcg
     4501 cctctcctcg atctctcggg cgttctcagc caagagcccg gaccgcagga ccccgcgggc
     4561 attgaggatg tcctgcagcc gccgctgcgc ctcgtcggcg gcttcggtca cccgttctct
     4621 cgccacgccg aacgcctggc cgacggtgct cccggccagg tcgtccgccg ttcgcagccg
     4681 ctggatggat cgccttgcca gctcgttgat gagtgtgttc gcctgcggcg agagagcctg
     4741 cgtcaccggg ggcggctgag ggcctgagcg ctgaaaggcg ttgctggcaa ccgccccgag
     4801 agcttgtccg atgaccgcgg gagccgcggc cacgacgcct tggccgatgg ccgaaagcgc
     4861 cgccggaatc actgatgcca tcaaatcacc cgctttcgtc gtcgctcttg gggttcagga
     4921 tgccgaagat aaggaggtct tcccagccgt cgcgggtccg cagggcctgg cgctcagtgc
     4981 cctcctgctc gaagccgacg gccttgagca gccggatcac cggctcgttc gaggcgcgca
     5041 ctctcgccgt cacgcggtgc agcatccact cgtcgaagac gatgcgccga agcgccctgg
     5101 ccgcgaaaag tgagagccct ttgccccggg ggtcgggaat gccgattccc acctcggccc
     5161 ggagattttg ccaatcgacg tgaaaaaggt cgatccagcc taccggcctg ccgtcttcgg
     5221 tgcggatcat ccacacgaac cacggcctcg aaagatcgac cggctcaagg tggacgaggc
     5281 gaaaaagagc gggatcttcc atccagcgcc gcaggatcgg cagttcctca ggcttcagcg
     5341 gcgtcaggtg catcgggaac cccttgcgcc gcgccggtcc gggccgattg ctccagataa
     5401 gccttcaggg cgcgcagcgc cttcaggttc tgttcgatgt cgtagaggag acctttcaga
     5461 cgaacctcct ccgacgacac ctccatcaac ttctgttgca gccagtctct cgattccaag
     5521 accctctctc ccctcacgcg gcatgatagg gcaccttttt cacggtgccc gcgaccttca
     5581 ccagcaggaa tccctcctgg gctccggcgg ttccggtcgt gttgcttgcg gcgtagccga
     5641 tcttgcccgt ggcatcgatg tcgaagatgc tggcgcctcc cgaatccgtc acttttagcg
     5701 cgtcgccggt ctgcccgaac gcgacgcgca ggagcaagtc gtgccacgcc gagttgtccg
     5761 cgccgctgat cgagacgcgg gcgcccgaag gcggcggggt gaaattcagg ccgatcgcca
     5821 cgcggcgatt gcctccctcc aggtactccc gcaggtagac gatgtcggag acgcccgcgc
     5881 cgatgtcccg ggcgatgtag aagtcctcga tgaccgagtt ggcgacgtcc aatcccatct
     5941 cccatcggcg aacgccgtct ttcagccact ggatgaacgg ctggtgcgtg gtgacgccgc
     6001 cgctggtggt gccgacgagt ttcaagacgc cttcatggga gatggcaacc ggcagactgg
     6061 gggagccgat ccaacgcgag aaccccgtgt cgggaatcag gttcccggac gcatcgacgt
     6121 tccagaagcc gcccgacacc ttggccatcg agagccggtc gctcttgagg ttcgcactgt
     6181 cgatgttgcc gttgaactcg ttgtagaggg tatcgaagtt gttgttgaga tgcgagcccg
     6241 aaggcacgtc cgccgccgcg aaggcggtgg gctttctcac ctgcgccacg gagcacgccc
     6301 tcctcaatag gtgatgctgg ccaggatctg caccgcgccg gtgtggaact tcgccttgag
     6361 cgcgacttcc cggttgggac tcgtgcccgt ggcggtgacg aacaagacga cgttgtctgt
     6421 tgccggtgcg gcgggctccg agatcggaac gaagggatgg cgctcggccc acacctccgc
     6481 ccagcggttg ctggaagttc cgttctttcg ctgactggcg gtggcatcgg gaaagaagtc
     6541 gctcgtcacg ccgcccgtgc ccagcagaag ggacgtcgtg ttcacgccat cggcgaaaac
     6601 gtcccacctc agcgtgtcgc tgcccagcgc ctgcacggcg ttcaccgccg gaaggaggtc
     6661 ccccgtcagg tcgatgtcct tcgcccacac cttcagccat ctcaggctgg acgaaccgag
     6721 gttcctcgtc tggttcaagt cgggcttcag gtcggtggtg agcgtattga cgttgatgcc
     6781 gtcggcgtac acgtcccatc ggtgcgttgc gtcgccgagc gcctgcgtca ggtcgttcac
     6841 gggcaggatg tcgcccgaac cggaaaggtt ccccgaggcg tcgatgctcc agaagccgcc
     6901 cgtcaccttg ttgaacgccg tccggtcgct ggccaggttg gcggcgctga tgctcccgtt
     6961 gaactcgttg tagatcgtgt cgaagttcgc attgacttgg gtcggatcga tgaccgtgtc
     7021 ctcaatgaag ttgtacggct tgttgatctg tgccacgtta ggccctcctc agcacgcgtc
     7081 ggggaatcgc ctcgacggcg aatccgtgaa tcgatcctga atcggttgat gagttgtttc
     7141 gaatcgtcac tgtgattgag gccccgaagt cctcccgcct gaacgattcc gagaatgttt
     7201 gaatgcgcga ggtagaccag cgcgagactc cccatatagc ccgaccccag aaggtcgcgc
     7261 cggaggcgcc gatgattgcg ctgctcatat ccatggttgc tcccgggccg ctctggtcga
     7321 tctggtagga caccgtccac ttggctccgg aggaatgtac atcgatccag aagcgccgga
     7381 acgccttgat gacggtgaac gagtccaagt cgtaagcttt actcgtgaag tgcgcgtcga
     7441 tcggctgccc gtcgtccgaa gtgccgcgat ctccgaagaa gaccagtccc tgcgatgcgt
     7501 cgccccaagt gatctcatgc ccgtcgccgg cgccgtccca tacggcgtac gcgtgcgccc
     7561 ggatgcccga aaagatcgtc caggtcttcg tctcaaggtc gaagacgaaa gtgagggcct
     7621 cggagttgtc caccgagctt tcgatcggcg tggtgagcca caggaagttg tcgtgcaccg
     7681 ccgccgcggc gacgctgaga ttgccgctgc gcaaaaggtc taggttcacc ttgtgtgaga
     7741 ggtgctccaa ggtcgttcca cgcatccggt agatgccgtc ggcccccaag aagtacgtcc
     7801 agatgccgtc ggtcgtaaca gagcgcggcg ccacgcagcc gatgcccgga aagatctctc
     7861 ggcgcaccca gttctcctgc cggtcgccga tcaatagatg cgtgctccgg tgcttgaaga
     7921 tgacaaggtc gccgaagctg gaggcgagcc cggtgatcca gtcgccggcg ccggaggagg
     7981 tctcgatgtc gatgaagttg gaggcgggcc aagcctgatg atcgtccagc tccgagaagt
     8041 acagccgcga cgggttggca ggatcgccgg ccaagaagag acggttttgg tgctgcacga
     8101 tgaactgcgc acgcggcgga ctcccggcca catccgtcac ggtggtgccg tcatactgct
     8161 tcaatgggtc caccgcgttc gtcgcaaaga gcaggtcctt ccactgctcg aagtgccact
     8221 cacggttggc ggtgaggccg gtcgcaatgt cggaaaacgt gccgttcacg tcgtctccga
     8281 ctttgagcgt cgtgccgacg ccgatcacaa gccgcttggt gccgtctgag cggtagaagc
     8341 ggacgatccc ctttaccggg tcgcttccca ggctcacggc gttgtaccgc tggctgccgc
     8401 cgcgcttttc gggcaccccg gtccggaaga ccatgtttcg gcagttgatg acctcgttgt
     8461 ccttgagaag gtgcgggtga atcgtccgat tcaccccgcc ggagaggtcc cggacattga
     8521 gcaggacagg acgcgccatc gcctagtccc ccgtccaccg cacggtggga tacgtctcgg
     8581 cagtccgtga catccccgcg cccagaagct ccagcttccg ccgctcgaac tggagcatga
     8641 aggcgctggc ccgctgaatg ttttcgtccg cgagcgccca ttgcgccgcg gcccagtatg
     8701 ccagtgcgta gtgatacgga gcgggaatgg ccggctcgtc ggttcccgcc gccatcagag
     8761 tcgggcttgc ctgatagaag agccgaatct ccttggcatc ggcggggatg ggatggaagc
     8821 cgagcttgac ggggttcaga cgcagataat acccaagcgg cgtcccctgc aagtctgggc
     8881 ttgcatcgac gatctcccga aagtctatgt ggcgcagctt gagcccgtcg tagtagacgg
     8941 ccaaggggcg catgaagtcg attgggaagc ctacgtcagc acctccgtat tcctgctggc
     9001 cgggaacgga catgaacgtg gcgagtttct cctcggcccc ggcatcgccg agcagctcct
     9061 cgatggcccg attcagccag ttgtggatgg tgagatcgtc tgtatgagtc gtcgatgcct
     9121 cgccgtacag cttgcggacc gcatcggtca gctcgtcgcg cgtcaccgtc tcacctccag
     9181 ccgaagtcca tggccagcgc cttcctggct tctttggcga aggccgcagc cacctcgtcg
     9241 gctgcacgct cctcggcacg ccgcctcagg tactcctcct cctccagctc ctccagtacg
     9301 tcggtgtgcc cgctccactg gtcggtgtag cgcaggtgat ggagcagccg ggcgtcgaga
     9361 accggcttct tccacgtcag gacgtgctcc agccggtccg tggcgtaggc gagcccactt
     9421 ttgagctgca ccaggaccgg cttcaccatc ttcaccagcc agcagccggg ctcgccaaaa
     9481 atgcggaggt ccgggtaaac ctcccggacc ctgcgctcga tgtcgtagac atcgccaacc
     9541 ggcatgcaca tcactcccaa acgcgaaaag acaccctcct gggtgccctt ttcaatggag
     9601 agcagcggct cgtttgatac tcgctcatgg tgctggcccg gtaagtgccg tgatatcgcc
     9661 cttcggcgtc agtcgtcgaa caatccccgc cgcagccagt cggcgaagta caactcccac
     9721 ccccgcgttc ctccggacgt ctgcagaagc gcatcatgca gctcctgccc gcgctttccc
     9781 gcgttgtagg ccgcctcgaa ggtcggtccg aacacgtcga ggaagaattg ccggctggcc
     9841 gtgtagctcg acatcgggga aaacgtgtcg tcggtcccaa accgttggtc gagccactcc
     9901 gcggcggtca tcaggcgctg cctcctacca aggtgtcgat gtaggcgttg accactccgg
     9961 acgtgggggc aacggagcag gtgaacagaa ggtcaatcac ttcgctgggg acatcgaggc
    10021 cggggaagtc ctggttcgtg atgaccttct tggacccggc ggggactgca atggtcccca
    10081 acgaggccgt aatcacgttc agcatttgca gccgcaccgt gacagtgact tcctggtcta
    10141 gctgattgtc cacatagatc gggactttgc cgccgcccca caccgtgcgg actaagtcga
    10201 acacgaactg cggatcggtg aacttgctgt gggtggcagt gtccctgatc gccagatcct
    10261 gagccagcac gccccgcagg ttctgcgggg tgatctgctc caacgcgcgg tatctcagct
    10321 cgaacactct cagcctcgtc tgcggagttg gagtctggtt gcgaaccgtt accacatagt
    10381 tccgcccgat aggcctgaac ggcggaatga actgtacgtc ctgagcccca agggctttcg
    10441 gcacagtcac acgggcgcgg gagatggcag gattctcccc tgcggtaccg tcccccggaa
    10501 tccaatcgaa ctgtgtcacg gtatcgccgt taattatgta ggccagcatg gcgataccgg
    10561 gcgacgccat caggtcaatc acgccggagc tgcggatccc accagctgcc aggggcgtcg
    10621 tggtaatggt cacgacttcg ggctgcacgg ttctcccaaa aagcgacact cgatcgacgg
    10681 ggagctggct ggcttcgtcg atgaacgtga ctgccatcgg atcactcctc acaatccgaa
    10741 gcggggagag cccgaaggcc ctccccgcca tccgcatgcc ttactccgtg atgtccttca
    10801 gcgctgcgtt cgccgccggg accgtgttga cgaggttgcc gtagaaggcg agcgtcgcct
    10861 cataggcgtc ctcggaagcg ttcgagccca cgatccggtg cagaatctgg ccgtcttcgt
    10921 cgagccagtc gaagtcggcc atctgggcga tgaagaacgt ggactcatca agcagccaga
    10981 cggtgttcag cggcgcctgc gagtccacga tcaacggata gccgttgaac tcgacgcccg
    11041 tgaacccgcc gtcaagctcc atcgtgttca cgtgacggcg gtcggcccgc accagcgcca
    11101 cgtacttcct ccgcacgccg tggctggtga tgccgatcga gatgcggcca ccggcccgtg
    11161 cctccgcctc gtcgtttgcc tgttgcagga gatcgagcga caggctgcga agcgtcccgc
    11221 cgttgtcaag cacgttcggg cggaaccagc cgttggcggc gtcgctgcgg tcaattccgc
    11281 cgaccgtgtt ggtctccgag ccgacccacg cttccagccc ctcgatctca aggccgcggc
    11341 tgtcggagag aaagatcgcg tcggtatcgg cggtagtgac ggctccgccc gcagagtcga
    11401 tcgtgatcgt cttgttggcg cggtcgatcg atacgaccgt acggtctccg ccgccggtga
    11461 tgtctgctcc cgaggagttc ttgatgtcga ttttcatgcc ctcgtagaac agattcatgt
    11521 tcgcgtcggc gtcgagctgg agcgtcgttg tagacgtatt cgaggcacag ccggcgatcg
    11581 tgccgaacga gtcgttgtag aactgccggt tcacgtcgcg gcgcaaatcg cgcatcatgc
    11641 cgtccatctc cgcccggacg gcccgaacga atgcgccggc gttgttccgg ctggccgcga
    11701 tcgtcggtcc cgtgatcttg atcgagccgt agatgtactt gggcttcaag atcaggtccc
    11761 tgtactgctg gtttcccgga gtcggcagcg ttccgccgtc ggaacgcgat ccaaccccgg
    11821 cgttgcggct gaaggagagc ggcgtaacga tctgccgacc gctgaagacg tcggttctgc
    11881 gctccatgcg gcggaggaag atcacccggt cgttgagctg ccggcggatc ggtccgagat
    11941 agaaatcctt caggaccgcg tcgaagttcg tgaggttctg ggccatgctt tctcactccc
    12001 gctactgttg gcgggccatc tgctccagga accgaatggc ggcctgatcc gcctctttca
    12061 tcgtctgagg cggctggtgc cggttggccg gcgaggcacc cctcgttccc tgaacgggcg
    12121 gcccgcctct cgtcttcgag ttgaggtatt gctgaatgac caggttgcgg atgtcgtcga
    12181 agtgctcaag gagcaccgcg gcccgcacct gcatcaccgg atcgtccaaa aggtgctcga
    12241 tccggtccac gatccggttc tccgtgatct tgacgccctc gaactccttg cgcgcccgct
    12301 ccagctcctc acgcatctgc cgctcgatcc gggcggtctc gcgctcctcc tcggcccgtt
    12361 tgatctcggc tacctgttgc ttgagctgcg tgtagtcctg gatcagctcg ggcggaaggt
    12421 tgagccccgt ctgctgagcc gctccggcct gggcctgggc ggcggtgaat ccgcgtgctc
    12481 gaagctggtc gtaggcggca aggtaggcgg caaactgcgg gtcggagcgg atgtaatcga
    12541 cgagaggctt gaattgctcg tattcccgca gcgcctgggc gtctcgctgg cgcttctgct
    12601 ggtagtcgaa gccctgctcc gcaaggttga tcagctcttc ctcggtgact tcgtgctctc
    12661 ggtcgcggtg acgaatcttg taccgcctcg gctcctgctc aggctgtggc ttgtcgccct
    12721 cggcctcaga agccgagctt tcggcctctt cgtccgcgaa cgcctgctga agcgcctgct
    12781 ctgcggcctc tccggcctgt tcgaacgtct gcacgtcatc cggcatcggg taagtccccc
    12841 ttgcgctgta gcaaatgccc tcggcgcgcg tgatccgctg tggcaaacgc cctcggcgga
    12901 aaccgaaaac gccgccccga aaggagcggc gtcaatcttt gcgtctgtta cgccggcggc
    12961 gggcctcccg gcgggccaac cggcggagaa cttcctcctc cgagccccgg tggtgccgga
    13021 gaaaccgccg gaacacctcc ggcttgcgct tccggaggaa gtgaaactgc gccaggctgc
    13081 tgaacggcac cctggatcac cgccgctctc tgtagatctt gctgggcaag ctgcatggcc
    13141 agcatttcgt gctggcggac gtgaatctcg aagagctgct tcgtcaaggg atcaagccgt
    13201 tcgaagtcat cggtcttccg gaaacgattg tgctcccgga tgtgcacctg gtggttctcc
    13261 cagtggttcg gaagttggcc tatgcccgcg gccatgcgct tgttctcaag ctcggcccgc
    13321 tgctcgtcca gcgtctcttc gtccacctcg ggctcgaaaa tgcccgcatc catcagcttc
    13381 aacgcccggc gccgctcctc agggtcgcgg atgtcgatga agcccgactc ggccagcgag
    13441 acgatgaact gctgctgcgc actcttcagc ttcggaagag cgctcccggc ctggacctcc
    13501 acgtccgtcg accggatgtc ggcggcgtag aagtcgatcg tctcagcgat cccgttgcgc
    13561 ccggcgatct ccaccgtgcg cggctccagg tagttttgct gagccaagca caggatgcgg
    13621 ctgccaattc ccgagaacgc cttctcgacg ttggccacca ccggcccgag cttcgtctcg
    13681 tcctgctcca aaaggagact cagcgccacg ccggaccgaa cccccggagg cggagcaccg
    13741 agcgaggttt cccgaacgcc catgatgtct cgcatagtct ccagatagac gctgatcagg
    13801 ttgaacagcg actgcggcaa gggcggcggc gtccagaacg tcggcggcac cgcacctcgg
    13861 taagagacca cctgccccgg ctcgtccgtg atccgctttt cgtcgatcgc accccaaggc
    13921 gacagaatct tgccggagga gaggatcagt gcttgctcct ggagcttcga gatcacccgg
    13981 ttgatctggc gctgaaggct gatgcagcct tcaaccagcg acatcggcca gaactggccg
    14041 ggaacgatga tgtgctgaag ctcgacgaac ggaaagcgcc tgtcggggtg gaagggcacg
    14101 ttcggatcac tgttgcgccg ctgggtcatc ggaaaagggc cgtcaaccag gagcttcccg
    14161 gacgccaccg caatcatccg cccgttcgga aaccgtggcg acggcctctc ccagtactcc
    14221 tttacgatgg cgtgatggtc gagcttcttg ctcgacgaag tgcggtcctc gacgtcgaag
    14281 ccccgggtga agccggaggc cacgaagtca gcaaagtcgg ccttcgacac gtcctccgga
    14341 ggcacgcgta cgccgtactg ctcttcgatc gactcgacgg tgcgaatctt ctcgtggatc
    14401 atccactccg cgccttttag cgtgcccgtg gagtccgccg ctggatcgac caccacctcg
    14461 aacggagagg ccgcttccac ggccacctca ccgacgaaga acgtgatcgc ccgatctccg
    14521 atgaactcgt cgatctgcgg acccgcctgc ccatcccaat atgtcttcat gaacgcctta
    14581 ccgcaggtga gcatccacag cagggcgaga ttgcgcacat cgtccagctc aaggtgccgc
    14641 cagaggtagt cgagcagctt cgatgcccgc cgcgcggcat tgatgtcgtc ttcctctccg
    14701 gtagcaggct tggcgaccat cacggggcgg ttcttcgtga gcttcgcaaa cagagcccac
    14761 acgaacgaca ggaggtagtt ggcgaccatg cgcaccctcc agggcggcga gggcggctcg
    14821 atcagcttgc cggtggccgg gtgcacgatg tgccactgct tgcccacgta gaaggcaagg
    14881 ttcattcgcc actgctcctc atagggccgg cgatgctcct tgccgatctc gtagcgctcc
    14941 tcaaccagcg cgatcgggct cgatcccttc accccgggcg tcatgatcgc catcccatct
    15001 cacctcccta gctgttgtaa taccgctcag caagctgcct cagagcgctc tgctcggcgt
    15061 cttcttcctc agcccggctc gaatgaccgg cagcgatctg cgcctgaacg tactcgccga
    15121 aactgcggct catcagccgg ttgagcagtg ccgttcgctc ggcgtcccac atcctccgct
    15181 cagaaagctg cagccactcg cgccacgccg cccatgccac cacgatcgcc agcacgatgt
    15241 aggcatcagg caccgacggc cacctcctct gcgtgcaccg actccatgtg ccgccgcagg
    15301 ttgtgcccgg cgagccgccc ggtgtagacc cgcccgcact gcgggcactc gacggtggcc
    15361 gtatcggcgg acgggcggtc gcctcggagc aggcttgggc gcaggcttcg gagccggtcg
    15421 aggacgaggt tcaggagtcg atcgtcttga gccgccgtcg ccacgatctc ggagacgcat
    15481 ccggaacaga taaagaccca ctcctcctcc acctccgtct cgcagtccac aaacggcccc
    15541 tgcggcgtgc agcggcagat cagacagcac cccggatgca tgtccggggc gtcgatgagt
    15601 ctcacgatgc tccctcctga cgcatgcggc ggcgacggcc acgggcggcc agctccgcca
    15661 tccgcgaggc gccgtgctcc tgccgcccga tgtgagccgc cagcgcctcc ggatcccgca
    15721 cgttgccgcg acgacggagc ttccgcacaa gcgcgcggaa ccgctccccc gtgccgagcg
    15781 ggggcttcct ctcttcggcc atcggcgttc acctcaccag tatccgggat cgttcgtgcg
    15841 gtttcgggca gcgaccaggc gctcaatatg acgagtagcc cgcgtcgttg ccgtgttcgg
    15901 cttgggctcc tccgggcgct gtgtcggctg tgggcgagcc atcagcaggt acctgagggc
    15961 atccacggcg tgatcgtcct gcttcagcgg cctctccggc tcgtccccga ccgcggtccg
    16021 ccgcggcgcc catcggtatc ccttgagctc ccggatgaga ttcgagcacg tccgatagat
    16081 gcgtagccga ggcgggtctc ccgacagcca ctcgtggatg cggttgatgc cggcgtccac
    16141 atcgttctga gcagggatca gcgttacgcc ctgttcgtga tagatgttgc cgaccgcctc
    16201 tcccgtcgga ccgcggttga ataccgacgg gtcggcgtag atcggcacct tcccgcgcct
    16261 gaggatctcc ttggcatgca tctccggcgg ccactcggcc tggtagtgct catcgtagat
    16321 gatcacattg ccgtcccagt ccactgctgc ccagacgacg gccgtcgggt tgcgcaggcc
    16381 gtagtcgaag cctgccagct tctcccatcc ctccggaatg ccgtcgggaa gtgggtcgat
    16441 gacgtgcttc gactcatcga actccgtgaa tatctgcccc tcgaacacgt cgaagctggc
    16501 atccaggtat ttcctcaccc actccggtgg gtaggacgag agaagcgact cgacgtatcc
    16561 cggcggcaga tgaacgttct cgcgggtggg cgcatggatc agggcgtagt cctgccgccc
    16621 ctccggcggt atcgcctccc gtgagacgaa ccgccggtac atccagtcat ggcccgccgg
    16681 gttggaggtg atccatcccc gccgcggccc gatcgggttc ctcagccggc tttgcagcat
    16741 caggaaaacg gtctccgcca cctccgatgc ctcgtcgatg tagaaccacg acaaattgag
    16801 cgatttcagc gtctcgtccg cattttcgag gtatctaaaa agcacctcgc tgccattggg
    16861 gaggatcagg tggccctccg atcgccgcca cgtcgagaga cgcagcagct cctgcggcaa
    16921 gcactcctcg aaaaacgtcc gctgagtggt gtctcgcagc tcgggatacg tctgccgggc
    16981 gatgacgccc aggctgcccg ggtgctccag cgccgtgacc agcgcctcca tacagccgca
    17041 gcgcgttttg cccgagccga accccccgat gtaggcccga taccgcgccg gcgagcggtg
    17101 gaactccgcc tgccggggca gcggcgtgta gtcaatggcc accgtctgca ccgtcatcac
    17161 ccgggcgcgg gatcctcacg acgatctcca gcccctcgcc atccggcccc gtgatcgcct
    17221 gcgccggcct cccccacgcc cgatccagca gcgcctccgc cgccgccacg cgggcacgcg
    17281 acggctct
//
LOCUS       DASVDU010000075        25122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1367306, whole genome shotgun sequence.
ACCESSION   DASVDU010000075 DASVDU010000000
VERSION     DASVDU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 25122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25122
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367306"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF226_04880"
     CDS             <1..426
                     /locus_tag="VF226_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7073354.1"
                     /translation="DLVHDALVRALQSKAVPRKADEVRPWAFRVLKNLFLDVRRRQKV
                     RAGYAREEALLNHASAHRSLDPVEALIVRQAYDSLSAHDREVICLIDILGLTYAEASA
                     VMDVPIGTVMSRISRARRAMLDIIEGSNVRPLRKHKQAK"
     gene            432..1202
                     /locus_tag="VF226_04885"
     CDS             432..1202
                     /locus_tag="VF226_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073355.1"
                     /translation="MAPLEPSDWELINAYADDELPAEDRRAVERRLAGDGAFAAALHE
                     VRSLKANLQALKPAAVQPHPGLARQRQLVRPVPMGGAAAAVAAALFAMAQLLPPSTAA
                     DWDEVTSNLHAQFSGNAYVLAPANPVVAVSTGRMGDLAAFDLSDSRLTLVDARNVQVS
                     GSTIVAMHYRGPSGCSLTIAVAAQSALKHVSARSSNALQAHWTAGGNHFHVVASGMDR
                     NRFEAIAAYVRAETLHQGGGKEMRIALRSATDHARPCA"
     gene            complement(1219..1527)
                     /locus_tag="VF226_04890"
     CDS             complement(1219..1527)
                     /locus_tag="VF226_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073356.1"
                     /translation="MVRKTFLAAVAGALVFAMSADAGEFGRLVDGKGAEETYYACTGC
                     HSDMLVAQQGLTRERWNELIDWMIDEQGMPELEPEERELILDYLAKNYGPDRPNFPLK
                     "
     gene            complement(1587..2888)
                     /locus_tag="VF226_04895"
     CDS             complement(1587..2888)
                     /locus_tag="VF226_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX7073357.1"
                     /translation="MSKDEKRPEQLGLHEFFAKDPIAADKAFFGRISHADRRGFLRNA
                     GLATMAAMVGANIPFHRNMPAGLIPAAFAAEDVLVGKDGLKLFNDRPVNAETPAHLLD
                     DPITPTNRHFIRNNGVPPEDMDPAKWKVKIDGEVENELNLSIDDLKNQFEVVTMALVI
                     ECGGNGRAYFNPPVSGNQWTVGAVGCANWTGVRLKDVLTKAGLKSSAVYTGHEGADTH
                     LSGDPKKVPISRGVPIAKAMADDVLIAFQMNDGPIHPMNGAPLRLVVPGWPGSCSQKW
                     LTRIWVRDKVHDGEKMKGTSYRLPNRPVAPGEKVATEDFVILERMPVKSIITFPENNA
                     NVKGETEVRGHAWSGDRTIDALDISIDFGATWQRAELDKPVNSGAWQNWRTNVKFPKP
                     GYYEVWARATDSAGVAQPFAVDWNPRGYANNMMHRVAVRVS"
     gene            complement(3013..3321)
                     /locus_tag="VF226_04900"
     CDS             complement(3013..3321)
                     /locus_tag="VF226_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073358.1"
                     /translation="MAANATVSAQSEPRASVPSSFKALFYVVGVADPGLLPRLIEPVA
                     KMGHVPSRVHASRESGDGSEVVVDLRLVDVNARTAQLVENALRATVGVRQVISVVEPA
                     "
     gene            complement(3641..4048)
                     /gene="mutT"
                     /locus_tag="VF226_04905"
     CDS             complement(3641..4048)
                     /gene="mutT"
                     /locus_tag="VF226_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HEX7073359.1"
                     /translation="MAKPLLLVAAAALVDRDKRVLITRRPPGKPMAGLWEFPGGKVHE
                     GETPEQALIRELFEELGIDVCPTCLAPLTFASHAYEDFHLLMPLYQIRTWDGVITPRE
                     GQEIRWVRVNRLAEYPMPPADLPLIPMLRDLLG"
     gene            complement(4076..5323)
                     /gene="argJ"
                     /locus_tag="VF226_04910"
     CDS             complement(4076..5323)
                     /gene="argJ"
                     /locus_tag="VF226_04910"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX7073360.1"
                     /translation="MGSTAKISPFAPASLPEVPEIAGVRFATAQAGIRYKGRTDLLLA
                     VLDPGTVAAGVTTRSKTCSAPVLWCRQQLAHGRARALVVNSGNANAFTGRKGHEAVAI
                     TAEAAAKAVGCSPEEVYIASTGVIGEPMDASRFAHLLTDLAAAARPERWEDAARAIMT
                     TDTFPKLAVRRVPMGSGEVTLAGFCKGAGMIAPDMATMLCFMFTDAPIAQPVLQRLVA
                     SSADRTFNCITIDGDTSTSDTVLLFATGQAAGRGVPEVRDAADPVLAPFAEALHSLMH
                     ELALFVVKDGEGLSKFVTVEVLGAEDDRAARRIAFSIANSPILKTAIAGEDPNWGRVV
                     MAVGKSGEAADRDRLAIWFGPHLVAREGERAPDYSEPVVAQYMKNGEITIRVDVGVGA
                     GRATVWTCDLTHDYVSINADYRS"
     gene            complement(5515..6384)
                     /locus_tag="VF226_04915"
     CDS             complement(5515..6384)
                     /locus_tag="VF226_04915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7073361.1"
                     /translation="MISKVRLGILAAALAGGLAFAPIHSSVADETVATVNGKTITEAD
                     IRLAEAEIGSDLGSLPEATKRRVLVEYLIENQLFAEAAEGDKLGSTPEFDGRMQYWRR
                     RALRDAYFEKSVKNAVSEADAKKLYDEQVKLLKPEEEVKARHILVDTEDQAKEIAEKI
                     AHGADFAEMAKQYSKDPGTKDEGGSLGYFSKGQMVPQFEEAAFKLDKGDVSDPVQTQF
                     GWHLIQVEDRRERKPPSFDEIKDRLIASMMHRKAQQIAADLRSKAQIEYVDPAIKEQV
                     EREKVPGAPAPKQ"
     gene            6751..9582
                     /gene="secA"
                     /locus_tag="VF226_04920"
     CDS             6751..9582
                     /gene="secA"
                     /locus_tag="VF226_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7073362.1"
                     /translation="MLSIGTIATKIFGSANDRKVKSYRATVAAINDLEPELEALTDEQ
                     LRSRTEMFRQQLKAGKSLDDILVPAFATVREAAKRTLGQRHFDVQLIGGIVLHQGKIA
                     EMKTGEGKTLVSTLPVYLNALAGRGVHVVTINDYLAKRDAEWMGEIYKFLGMSVGCIV
                     HELDDAQRRAQYACDITYGTNNELGFDYLRDNMKMSRDEMVQRGHYFAIVDEVDSILI
                     DEARTPLIISGPVEDRADLYTAIDALIPRLSKEDYELDEKQRSVSLTESGNEKIEKML
                     EAAGLLKGSSLYDIENVTVVHHVNQALKAHTLFQRDRDYIVKGGQVVIIDEFTGRMMP
                     GRRYSEGLHQALEAKEKVQIQPENQTLASITFQNYFRLYEKLAGMTGTAATEADEFLE
                     IYGLDVIEIPTNLPVARLDEDDEIYRTADEKWDAIIREIESAQKRGQPVLVGTVSIEK
                     SELLSERLKKKGVPHQVLNARYHEQEAYIIAQAGVPGAVTIATNMAGRGTDIQLGGNA
                     QMRIQQETAGLSDEEKAKRIEEIKAEIERNREIVLKAEEVVEIEPAKGNKPAKTITKP
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGRSKFYLSLEDDLMRIFGSNRMDGVLRT
                     LGLKEGEAIIHPWMNKSLEMAQRKVEARNFDIRKQILKYDDVMNDQRKVIFDQRIDIM
                     GMDDVSETITDMRHEVIDELVKRHIPENAYAEQWDAEGLQTEVKNIFGLDLPIVEWAA
                     EEGIADQEIRERLIEAADAKARQKEADIGPEMFRYIEKMVLLQTLDHLWREHLVTLEH
                     LRQVIGFRAFGQRDPLNEYKGEAFILFQNMLAKMREAVTGQLMHVELATPQEQQSMFE
                     PAELPPMEAHHIDPVSGVDELAMADAALAAEELAYANGPDRRAPLQSRRGADRIDPKD
                     PATWGRVSRNAPCPCGSGKKYKHCHGRHS"
     gene            9799..11541
                     /locus_tag="VF226_04925"
     CDS             9799..11541
                     /locus_tag="VF226_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7073363.1"
                     /translation="MRILGLVGATHDSGLALLEDGIPAIVLEEERFSRRKRTKSFPRR
                     ALSLALEGSAQRLREIDVITTPWDVSRLRLTFLQALVGHLPWSLSLVRRSARSLQDND
                     IVLLNAYFARKFKRYFGIRKLPPIVNVGHHDSHAAVYFVSPFDEAAVLVMDGYGDDAS
                     TSAYIGQGRSLTRLWRTGIFNSLGMVYTFVTMHLGFGFSEEGKVMALAAYGDDTYVDA
                     FRNLIRPMPDGSYQVNFKYFSYDAFGELRPFTPCFLEAFGPPRQSGEELNDRHRAIAR
                     GLQTLTEEIVLHMARELRRRTGSRNLVLSGGVALNCVANARVLEEAGFDRIWVPPCAS
                     DTGAPLGSALWHYHQTLGNERRMELTHPFYGPLYDDSDIEKALAEAGLSARRMSEPEL
                     LDRVARDLASGKIVGWFQGRSEMGPRALGNRSILADPRRLEMKDIINARVKHREPFRP
                     FAPAVLIERASEFFHIDQPDPFMTLAPRVRAEKARLIPAAVHVDGTGRIQTVERSANP
                     RYYGVIEAFGKLTGVPVLLNTSFNRQEPIVETPQHAVSCYLRTGLDVLVMGNYYTTDR
                     NHADTVPERSMLAA"
     gene            complement(11542..12009)
                     /locus_tag="VF226_04930"
     CDS             complement(11542..12009)
                     /locus_tag="VF226_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073364.1"
                     /translation="MVTRILAAAAAAFLAMPASAANLPDWDVTDVCRQDSAPGQCRIF
                     EAQARNAITGSWAVLPTQVQDACIAATSDPADHSWRTLADCIELEVLRAKAKRTIATM
                     ATPTEPEPPAPAVTEPTTIEPQTMERAPQPAEMTSGSAEPATTPPAPAAGMAQ"
     gene            12121..12936
                     /gene="map"
                     /locus_tag="VF226_04935"
     CDS             12121..12936
                     /gene="map"
                     /locus_tag="VF226_04935"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006697403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7073365.1"
                     /translation="MSNLDLSHADQRDGRIRLYGPDDFAAMRKAGRLAAEALDMLVPH
                     VKPGVTTEYLDELIFEFAMDHGAIPATLNYRGYEKSICTSVNHVVCHGIPGPKPLREG
                     DIVNIDVTLIVDGWYGDTSRMYPVGEISRRAQRLLDVTYEALMRGIAAVKPGHTTGHI
                     GAAIQTYAEAERCSVVRDFCGHGVGRVFHDRPNILHYGNPGEGPELKPGMIFTIEPMI
                     NLGKPHVKILSDGWTAVTRDRELSAQYEHTVGVTETGCEVFTYSPAGLHQPGR"
     gene            12974..13711
                     /gene="radC"
                     /locus_tag="VF226_04940"
     CDS             12974..13711
                     /gene="radC"
                     /locus_tag="VF226_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX7073366.1"
                     /translation="MSNSDESSFTGRPRQGAFFEAPLPSHVGHRQRLRERFKTGGPDA
                     LPDYELLELLLFRAIPRRDTKELAKRLIARFGSFAEVINAPEHRLAEVKDVGEAVITE
                     LKIVRAAALRLMRGEIIKRPALSSWNQVVAYCRAAMGFEAREQFRILFLDKRNQLITD
                     EVQQEGTVDHTPVYVREVVKRALELSATALVLVHNHPSGDPTPSRADIEMTKQIIEAA
                     RPLGITIHDHIIVGREGYVSLRGLRLI"
     gene            13858..14826
                     /gene="trxB"
                     /locus_tag="VF226_04945"
     CDS             13858..14826
                     /gene="trxB"
                     /locus_tag="VF226_04945"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7073367.1"
                     /translation="MTQTKHARVLIIGSGPAGYTAAIYAARAMLKPVLIQGIQPGGQL
                     TITTDVENYPGFAKPIQGPWLMEEMKAQAENVGTEIIMDHITKVDLKQRPFRLEGDSG
                     DIYTCDVLIICTGAQARWLGLPSEETYKGYGVSACATCDGFFYRGKEVIVVGGGNTAV
                     EEALFLTNFANRVTVVHRRDSFRAEKILQERLFANPKIEVLWDTTVEEILGTTDPKTV
                     TGVRLKNVKTGTLSERPVDGVFIAIGHAPATELFKNQLEMKPSGYIITKPDSTATSIP
                     GVFAAGDVKDDIFRQAVTAAGMGCMAALEAERYLAGQAGTASIAAW"
     gene            15028..15918
                     /locus_tag="VF226_04950"
     CDS             15028..15918
                     /locus_tag="VF226_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7073368.1"
                     /translation="MDWDKLRIFHAAAEAGSFTHAGEVLRMSQSAVSRQVSALERDLG
                     VALFHRHARGLVLTEQGEMLYRTATEVMNKLQTAEMLLSDTTTKPSGDLRIAAPVGLG
                     TVWVTQRLREFMELYPDIRVELVLNDDQIDISMREADVAIWTREPEHADLIRRPLFTM
                     SVRAYASTQYVRMHGAPQSLEDLEKHRFVSYSGTPAMHLSAIRALETIGRDGQEPRVP
                     VFTANSIVALKYAIRAGIGVGLIPDYLTGDDTDLVTVLNDVQFPRIQIIFVYPEELRT
                     SKKIQVLRDFLVSKARSLKT"
     gene            16219..17619
                     /gene="leuC"
                     /locus_tag="VF226_04955"
     CDS             16219..17619
                     /gene="leuC"
                     /locus_tag="VF226_04955"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7073369.1"
                     /translation="MARTLYDKIFEDHIVDRQPDGTCLIYIDRHLVHEVTSPQAFEGL
                     RLANRKVRAPEKTLAVVDHNVPTSDRSRGIDDPESRTQVEALAQNAKDFGIEYYNELD
                     VRQGIVHVIGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQ
                     RKAKNMRITVDGKLPKGVTAKDIILSIIGEIGTAGGTGHVIEYAGEAIRALSMEGRMT
                     VCNMSIEAGARAGMIAPDEKTFEYIKGRPKAPKGAAWDMAMRYWETLHSDEGAVFDRE
                     VKLDAANLPPVVTWGTSPEDVVSVTGVVPDPDSVADEAKRASMKRALEYMGLTPGTKI
                     TDIKLDRVFIGSCTNGRIEDLREVARIAQGKKVAPHVYAMIVPGSGLVKQQAEAEGLD
                     KILKEAGFDWREAGCSMCLGMNPDQLKPRERCASTSNRNFEGRQGRLGRTHLVSPAMA
                     AAAAIAGHFVDVRTWR"
     gene            17693..18499
                     /locus_tag="VF226_04960"
     CDS             17693..18499
                     /locus_tag="VF226_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7073370.1"
                     /translation="MSEPRSLVQQQFGAHAAGYVSSVVHAKGESLQRLVDLTRPEKHW
                     RALDIATGAGHTALTFASQVAHVTASDITDEMLAEAEKLAKSRGIANVSFEKADAQTL
                     PFPDGGFDLVTSRIAPHHFPDIPKFVAEAYRVLKPGGVFGLVDNIAPDENTTPGFGAE
                     ALRAAADAYNDFEKIRDPSHGRCLTIGEWRDVTVGAGFVIEHLEKLSKSLEFAPWAER
                     MGAGPETIARLQAILNDSSPALRAYLRPEQRDGALYFTLDEVILVARRPA"
     gene            complement(18504..19427)
                     /locus_tag="VF226_04965"
     CDS             complement(18504..19427)
                     /locus_tag="VF226_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7073371.1"
                     /translation="MTSDVQLFDLGLLARRRDRAAPNLAEHDFLLQRVAEDFVIRIGA
                     VQRQFASAVDLGAHTGLLSRSLRQLPNVSPLVSAELSWQMLEQCPPPRVLIDFEALPF
                     ADESLDLVVSALVLQYVNDLPGTLAQIRRALKPDGLLLAAVLGGQTLQELREAFLLAE
                     AEVEGGASPRIAPFADVRDFGSLLQRAGFALPVTDTDVVTVTYKSPLALMHELRGMGA
                     TNMLVARRRTPLRRSTLIRACEIYIERHGRPDGRVPATFEIITLTGWAPHESQQKPLR
                     PGSAQVRLADVLGTREISAGERAPLAPPKKP"
     gene            19525..20292
                     /locus_tag="VF226_04970"
     CDS             19525..20292
                     /locus_tag="VF226_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEX7073372.1"
                     /translation="MTILERIRHHSKRAANAFADIVVPPTCLYCRRMLAVHDALCAQC
                     WRSIKFIRPPLCDRLGIPLPYDIGGRAISAQAEAHPPEYDRARAVAHFDGVLRDLIHQ
                     LKYADSHIARRLFGRWLAEAGAELLRDADLIVPVPLDRWRLLKRRFNQAAILSRELHR
                     LTGTPWDPLVLLRSKRTATQVGLTRDQRRRNVQGAFSVDPARANAVENRAIVLVDDVI
                     TTGATVEACARVLKRAGAARVDVLALGLVTPDSMITP"
     gene            20383..20640
                     /gene="grxC"
                     /locus_tag="VF226_04975"
     CDS             20383..20640
                     /gene="grxC"
                     /locus_tag="VF226_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEX7073373.1"
                     /translation="MSKITIYTTIFCPYCVMAKQLLKRKGVTFEEIDVTGKPALRQEM
                     AAKAGGRSTVPQIWIGRTHVGGCDDLYALDRTGQLDRMLAD"
     gene            20662..21540
                     /locus_tag="VF226_04980"
     CDS             20662..21540
                     /locus_tag="VF226_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7073374.1"
                     /translation="MPAATNGRKFRAGIVQMCTGRDVARNLAELSRLVREAVAAGAQY
                     VQTPEVSVLMERDRDRLFVETRREEGNPAIAHCQALARELGIWLHLGSMGILVKPDKI
                     ANRSFLFTPEGRIAARYDKIHMFDVDLPGGESYRESRRFEPGSQAVVADLPWGKLGLT
                     ICYDMRFPSLYRALAKAGAVFIAVPSAFTVPTGQAHWHALLRARAIEAQCFIFSAAQA
                     GQHEDGRKTYGHSLIVSPWGEVLAEADGENPSVILADVDVDAVDEARRRIPSLQHDRA
                     FEIVQAQAAADSDGES"
     gene            21537..21983
                     /locus_tag="VF226_04985"
     CDS             21537..21983
                     /locus_tag="VF226_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX7073375.1"
                     /translation="MIRYSLQCNKQHTFEAWFKSSTDFDLQARRNLVTCPECGSTKVS
                     KALMAPNVSTGRSKSGEVTVANPEAAAALEKRRELMALLRRLRAEVEKNAEYVGPRFA
                     EEALKMHYEEVEKRGIYGEATPDETRELIDEGVEFFPLPRLPEDQN"
     gene            complement(22013..22753)
                     /locus_tag="VF226_04990"
     CDS             complement(22013..22753)
                     /locus_tag="VF226_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073376.1"
                     /translation="MSISGAGEHGSVTLVTGVSRGIGTAIARELAKRGHHVVGLARTQ
                     PEAFEGDFYPVDLADAGATAAVLKEITRKKRVLRLVNNAGISRIAGAEATTLDDFDAI
                     MAVNVRSVMQCMQAVLPTMREAKFGRIVNIGSRAALGKDGRLVYGASKAAVVSMTRTV
                     ALEYAAYGITANCIGPGPIETDMIRKGYPVGSEARTAFTRQVPVGRFGQPEEIAHACA
                     YFLSEEAGFTTGQVLYVDGGVSVGQALI"
     gene            22951..23979
                     /locus_tag="VF226_04995"
     CDS             22951..23979
                     /locus_tag="VF226_04995"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7073377.1"
                     /translation="MGYKVAIVGATGNVGREMLNVLAERQFPADEVFPIASRRSIGTE
                     VSYGEKTLKCFDLEQFDFKKADFCLMSAGGSVSKEWAPRIAATGCTVIDNSSAWRYDQ
                     DVPLIVPEVNAEAVQNFRKKNIIANPNCSTAQMVVVLKPLHDYATIKRVVVSTYQSVS
                     GAGKEAMDELWHQTKGKYVPGQEVEPKKFPKEIAFNVIPHIDVFMEDGYTKEEWKMMA
                     ETKKILDPKIKVTATCARVPVFVGHSEAVNIEFEKPITVEEARNILREAPGVMVVDKR
                     EPGGYVTPVEAAGEFATFVSRIREDATVENGLAMWIVADNLLKGAALNTVQIAETLIN
                     RNLLANAA"
     gene            complement(24062..24493)
                     /locus_tag="VF226_05000"
     CDS             complement(24062..24493)
                     /locus_tag="VF226_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7073378.1"
                     /translation="MHLLHRASQCASDIFQAEIDGDLTPRQFAILLTVAQNEGLSQTH
                     LVERTGIDRSTLADVIRRMLKKGLIQRRRTREDARAYAVRLTDEGWRVLKKAEPLARR
                     ADERLLSVLSGAERDRFLKSLTEIVRFLEAEEGLRREAKSA"
     gene            complement(24715..>25122)
                     /locus_tag="VF226_05005"
     CDS             complement(24715..>25122)
                     /locus_tag="VF226_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEX7073379.1"
                     /translation="LGRIPVMPLVSGIFVVAFGALTLILHDAVFIKLKPTIVNALFSI
                     ILFGGLLLDRPLLRPLMGDMFQLTDRGWVILTVRWACFFAFLAVLNEIVWRNFSESFW
                     ISFKIWGIMPLTMAFALAQMGILKRYQAEHQSS"
BASE COUNT         4777 a         8007 c         7898 g         4440 t
ORIGIN      
        1 gacctcgtcc atgatgccct ggtgcgggcg ttgcagtcga aggcggttcc gcggaaggcc
       61 gacgaggtgc ggccctgggc gtttcgcgtc ctcaaaaacc tgtttctcga tgtgcggcga
      121 aggcaaaagg ttcgtgccgg atatgcgcga gaagaagcac tcctcaatca tgcctccgcc
      181 cataggagcc tagatccggt cgaagcattg attgtccgcc aagcctacga cagcctctcc
      241 gctcacgacc gcgaagtgat ttgtttgatt gacattctcg ggctgacgta tgcagaagcc
      301 agtgcagtga tggacgtccc gatagggacg gtgatgagcc ggataagccg cgcgcggcgt
      361 gccatgctgg acatcatcga aggctcgaac gttcgccctc tacggaagca taaacaagcg
      421 aagtgaaacc gatggccccg ctggagccca gcgactggga actgatcaac gcatatgccg
      481 atgatgagct gcctgcggaa gaccgcaggg ccgtcgaacg ccgacttgct ggcgacggtg
      541 cctttgccgc cgcgcttcac gaagtgagaa gcctcaaggc taacctgcag gcgctgaaac
      601 ccgccgctgt acagccgcat ccagggctag cacggcagcg gcagttggtt cgacctgttc
      661 cgatgggggg ggccgcggca gccgtggcgg cggccctctt cgccatggca caacttctgc
      721 caccttcaac ggcggccgat tgggacgagg tcacgtccaa cctgcatgcg caattttccg
      781 gaaacgccta tgtgcttgcg cccgcgaacc ctgttgtggc cgtttcgacc ggccgcatgg
      841 gtgatcttgc tgcgttcgat ctctcggact cccggctcac gctcgtggac gcgaggaacg
      901 tgcaggtgag cggatcgacc attgtcgcca tgcactatcg cgggccgagt gggtgcagcc
      961 tcaccattgc cgtggccgcc cagtccgcac tgaagcatgt ttcagcgcgg tcctccaatg
     1021 ctctccaggc ccattggacc gccggcggca atcacttcca cgtcgtcgcc tcgggtatgg
     1081 acaggaaccg cttcgaggcg atcgccgcat acgtgcgggc agaaactttg catcagggcg
     1141 gcgggaagga aatgcgcatc gcgctccggt cggcgacgga ccacgcacga ccctgtgctt
     1201 aaactctgtt gctgagtttc acttcagggg gaagttgggg cggtcggggc cgtaattctt
     1261 ggcgaggtaa tccagaatca gctcgcgctc ctccggctcg agctcgggca tgccctgctc
     1321 gtcgatcatc cagtcgatca gctcgttcca gcgctcgcgg gtcaacccct gctgagcgac
     1381 gagcatatcg gaatggcagc ctgtgcacgc gtaataggtc tcctcggcgc ccttgccgtc
     1441 cacaagacgg ccgaactcgc ccgcatcagc cgacatcgcg aacactagtg cgccggcgac
     1501 cgcggccaaa aaagtctttc gaaccatgtg tccttccgga agtctgcgtt caggggcggg
     1561 cgcctcgccc gccccgctaa tgcgtttcag ctcacgcgta ccgcaacgcg gtgcatcatg
     1621 ttgttggcat agccccgcgg gttccagtcg acggcgaacg gctgggcgac accggcgctg
     1681 tcagtggcgc gggcccagac ctcgtagtaa ccgggcttcg ggaacttgac gttcgtgcgc
     1741 cagttctgcc acgcgcccga attgactggc ttgtcgagct cggcccgctg ccatgtcgcg
     1801 ccgaagtcga tcgagatgtc gagcgcatcg atggtccggt cgcccgacca ggcgtgcccg
     1861 cgaacctccg tttcgccttt tacgttggcg ttgttttccg ggaatgtgat gatcgatttc
     1921 acgggcatcc gctcgagaat cacgaagtct tcggtcgcga ccttctcacc tggcgccacc
     1981 ggccggttgg ggagccggta agaggtgccc ttcatctttt cgccatcgtg gaccttgtcg
     2041 cggacccaga tgcgggtgag ccacttctgc gagcaggagc ccggccagcc gggaacgacg
     2101 agtcggaggg gcgcgccgtt catcgggtgg atcggtccat cgttcatctg gaaggcgatc
     2161 agcacgtcgt cggccatggc cttggcgatc ggcacgccgc gtgagatcgg cacctttttc
     2221 gggtcgcccg acaggtgcgt gtcagccccc tcgtggccgg tatagacggc gctcgacttg
     2281 agcccggcct tcgtgagcac gtccttcagg cggaccccgg tccagttcgc gcagccgact
     2341 gcgccgacgg tccactggtt gccggacacc ggcgggttga agtatgcccg gccgttcccg
     2401 ccgcattcga tgaccagggc catcgtgacg acctcgaact ggttcttcag gtcgtcgatg
     2461 ctgaggttga gctcgttctc gacctcgccg tcgatcttca ccttccactt ggcgggatcc
     2521 atgtcctccg gcggcacgcc gttgttgcgg atgaagtgac gattcgtcgg agtgattgga
     2581 tcatcgagca ggtgcgccgg cgtctcggcg ttcaccggcc gatcgttgaa gagcttcagt
     2641 ccgtccttgc ccaccagcac gtcttcagca gcgaacgccg ctgggatcag ccccgccggc
     2701 atgttgcggt ggaaggggat gttggcgccc accatcgccg ccatggtggc aagtcctgcg
     2761 ttgcgcagga aaccgcgccg gtccgcatgc gagatgcggc cgaagaaggc cttgtccgca
     2821 gcgatcgggt ccttcgcaaa gaactcgtga agtcccaact gctcaggtcg cttttcgtcc
     2881 ttgctcatac ctttcctccc ggagttgctg ccgggagtca acgcacgagc cgagcggatt
     2941 attccatggc attggctttg cttcggctcc gatccgccga gcacgaagat ttgagcagag
     3001 caggatcgcg cgtcaggccg gctcgaccac ggagatcacc tggcgcacgc ccacggtggc
     3061 tctcagcgcg ttctcgacga gctgggccgt acgggcattg acgtccacta ggcgaagatc
     3121 gaccaccacc tccgagccgt cgcccgactc gcgagatgca tgcacgcgcg agggcacgtg
     3181 acccatcttg gcgacgggct cgatcaatcg cggcaggagg ccgggatcgg caacgcccac
     3241 gacgtagaac agagctttga atgaggaggg gacggaagcg cgcggctcgg actgagccga
     3301 cacggtggca ttcgcagcca tgacgagaaa tccttcacat gcgatgagga gaaaagcgcg
     3361 aaaacgtatc ccggcagccc ttaggcaacc ggggcgctaa ttcgccgaat gatcgcgatg
     3421 cgaagtgagg tgtgcatgtg cgagagaata gcgccggctt gagagaactt caactagttc
     3481 tgtactttgc tggccgagca gtcgacccga accgattgac cgtccaattc cggtcatccc
     3541 ggcgcctcgt cgccgggatg aacctgaccc cagatgccgg agcaagcata ggggtagatt
     3601 ccggtgacaa gcaccgggat gactattttg gggtgcgcac tcacccaagc agatcccgca
     3661 gcatcgggat cagcggcaga tcagccgggg gcatcggata ctcggcgagc cggttcactc
     3721 gcacccacct gatctcctgg ccttcccgcg gggtgatcac gccgtcccag gtccggatct
     3781 ggtagagcgg catcagcagg tggaagtcct cgtacgcgtg gctcgcgaat gtcagcggcg
     3841 cgaggcaagt ggggcacacg tcgatgccga gctcctcgaa cagctcacgg atgagggcct
     3901 gctccggcgt ttccccctcg tgcactttgc cgcccgggaa ctcccacagc ccggccatag
     3961 gcttgccggg cggacgccgc gtgatgagaa cccgcttgtc acggtcgacg agcgcggcag
     4021 cggcgacaag gagaagaggc ttggccattc gtgtttggcg gttcgcggcg ccctgtcagc
     4081 tccggtaatc ggcgttgatc gagacgtaat cgtgcgtcag gtcgcaggtc cagaccgtcg
     4141 cccgaccagc cccgacaccg acatcgacac ggatcgtgat ctcgccgttc ttcatatact
     4201 gggcaacgac cggctccgaa taatccggcg cgcgctctcc ctctcgggca acgagatgcg
     4261 ggccgaacca gatggcgagc cggtccctgt ccgccgcctc tcccgatttg ccgaccgcca
     4321 tgaccacgcg gccccagttc gggtcttcgc cggcaatcgc ggtcttcagg atcggggagt
     4381 tggcgatgga aaaggcgatg cgccgggcgg cgcgatcgtc ctcggcgccg aggacctcga
     4441 ccgtgacgaa tttcgacagg ccttcgccgt ccttcacgac gaacagagcc agctcgtgca
     4501 tcaagctgtg cagcgcctcg gcaaagggtg cgaggaccgg gtcggctgca tcacgcacct
     4561 cggggacgcc gcgccctgcc gcctgaccgg ttgcgaacag cagaaccgta tcactggtcg
     4621 aggtgtcgcc gtcgatggtg atgcaattga aggtccggtc ggcgctggag gccacgagcc
     4681 gctgcagcac cggctgcgcg attggcgcgt cggtgaacat gaagcagagc atcgtcgcca
     4741 tgtccggcgc aatcatgccg gcgcccttgc agaaccccgc aagcgtgacc tcgccggaac
     4801 ccatcggcac gcgacggaca gcgagtttcg ggaaggtgtc cgtggtcatg atggcgcggg
     4861 ccgcatcctc ccagcgctcg ggacgagccg cggccgcgag atccgtcagc aagtgcgcga
     4921 aacgcgaggc gtccatcggc tctccgatga cgccagtgga ggcgatgtag acctcctcgg
     4981 ggctgcagcc cacggccttg gcggccgcct ccgccgtgat cgctaccgcc tcgtggccct
     5041 tgcgaccggt gaaagcgttg gcatttccag agtttaccac gagcgcacga gcccggccgt
     5101 gcgcgagctg ctggcggcac cagaggacgg gagccgagca ggttttggat cgcgtggtga
     5161 ccccggcggc aaccgttcca ggatcgagga cggccaaaag cagatccgtg cggcccttgt
     5221 agcggattcc tgcctgcgcg gtggcaaagc gcacacccgc aatctcggga acctccggca
     5281 ggcttgccgg cgcaaacggg gaaatcttgg cggtgctgcc caacgtctca cctcccagcg
     5341 aggtatcgcc aaaccaggtc gaaggagccg tgagcacgcg gtcgagcttc gaacgagcag
     5401 gcgacgccga accggccacc acgaaccaga tcggacgggg taaatcaata ggcatgaaag
     5461 aagaggccgc ccctgaggac ggcccctgac actgctatcg ccagctcgcc gcggctactg
     5521 cttcggagcc ggtgcgccgg gcaccttttc gcgctcgacc tgctccttaa tggccgggtc
     5581 gacgtactcg atttgtgcct tgctgcgcag atcagcggcg atctgctggg ctttgcggtg
     5641 catcatcgag gcgatcagac ggtccttgat ttcgtcgaag gaaggcggct tacgctcgcg
     5701 gcggtcctcg acctgaatga gatgccagcc gaactgggtc tgcaccgggt ccgagacgtc
     5761 gcccttgtca agcttgaacg ccgcctcctc gaactgcggc accatctgac ccttgctgaa
     5821 atagccgagg gagccgcctt cgtccttggt gccgggatcc ttcgagtact gcttcgccat
     5881 ttcggcaaaa tctgccccgt gggcgatctt ttccgcgatc tccttggcct gatcctcggt
     5941 gtcgacgaga atgtggcgcg ctttcacctc ttcctcgggc ttcagcagct tgacctgctc
     6001 gtcgtagagc ttcttggcat ccgcctcgct caccgcgttc ttgacgctct tctcgaagta
     6061 ggcgtcgcgc agggcgcggc ggcgccagta ctgcatgcgg ccgtcgaact ccggggtgga
     6121 gccgagcttg tcgccctccg ccgcctccgc gaagagctgg ttctcgatga ggtactcgac
     6181 gagaacgcgg cgcttcgtgg cctccggcag gctcccgagg tcggagccga tttccgcttc
     6241 ggcgagcctg atgtcggctt ccgtgatggt cttgccgttc acggtagcca ccgtttcgtc
     6301 tgccaccgag ctgtggatcg gggcgaaggc aagcccaccc gccagcgcgg cagccaggat
     6361 gcccaatctc actttagaga tcacgtgcga ggttccttgt tgatgatcgg acgggcagcc
     6421 acgaacatag ccgggcgacc tgaacgccgt tggttagaac gcaagttagc ccgttggtta
     6481 gacgggcctc gtggctttgc caagttaact ttggctcacg tggcggaatt tcggcgaagt
     6541 ggcggcacga ccaactgtcg cgttcgattg attgacattt gcgggcccgg cttcttatct
     6601 ctcccccaac gagtggcatt ctccggccgc cggtaccctg ctcgcgccct gtttgcgctc
     6661 tatccggtca gcttcgtgct cctttccgga cgcgagcacc tatgaccggg acgtccacgg
     6721 cgctggaatt gcctgcaagg aacgatacac atgctttcaa tcggcacgat cgcgaccaag
     6781 attttcggtt cagcaaatga tcgcaaggtc aagtcgtatc gcgccacagt tgcggctatc
     6841 aacgacctgg agccagagct cgaggcgctg accgacgagc agctgcgctc ccgcaccgag
     6901 atgttccggc agcagctgaa ggccggaaag tcgctggacg acatcctcgt accggccttc
     6961 gcgaccgtgc gcgaagcagc caagcgtacg ctgggccagc gccacttcga cgtgcagctc
     7021 atcggcggaa tcgtgctgca tcagggcaag atcgccgaga tgaaaaccgg tgaaggcaag
     7081 acgctggtct caacgctgcc ggtctacctg aatgcgctcg ccggacgcgg cgtccacgtc
     7141 gtcaccatca acgactacct ggccaaacgc gacgccgagt ggatgggcga gatctacaag
     7201 tttctcggca tgtccgtcgg ctgcatcgtg cacgagctgg acgacgccca gcggcgcgcc
     7261 cagtatgcct gcgacatcac ctacggcacc aacaacgagc tcgggttcga ctatctccgc
     7321 gacaacatga agatgagtcg cgatgagatg gtccagcgcg gccattactt cgccatcgtc
     7381 gacgaggtgg actcgatcct catcgacgaa gcccgcacgc cgctcatcat atccggcccg
     7441 gtcgaggacc gtgccgatct ctacaccgcc atcgacgcgc tcattccgcg gctgtcgaag
     7501 gaggactacg agctcgacga gaagcagcgc tccgtatcgc tgaccgagtc cggcaacgag
     7561 aagatcgaga agatgctgga agcggccggc ctgctcaagg gctcgtcgct ctacgacatc
     7621 gagaacgtga ccgtggttca ccacgtgaac caggccctga aggcgcacac gctcttccag
     7681 cgcgaccgcg actacatcgt gaagggcggc caggtcgtca tcattgacga gttcactggc
     7741 cgcatgatgc ccggccggcg ctattccgaa ggcctgcacc aggccctcga ggccaaggaa
     7801 aaggtgcaga tccagcctga gaaccagacg ctggcctcga tcaccttcca gaactacttc
     7861 cgcctttatg agaagctggc cggcatgacc ggcacggccg cgacggaggc tgacgagttc
     7921 ctcgagatct acgggctcga cgtgatcgag atcccgacga acctgcctgt cgcccggctc
     7981 gacgaggacg acgagatcta ccgcaccgcc gacgagaagt gggacgcgat catccgcgag
     8041 atcgagtcgg cccagaagcg cgggcagccc gtgctggtcg gcaccgtgtc gatcgaaaag
     8101 agtgagctgc tgtcggagcg cctgaagaag aagggggttc cgcaccaggt cctcaacgct
     8161 cgctatcacg agcaggaggc ctacatcatc gctcaggccg gcgtgccggg cgccgtgacg
     8221 atcgccacca acatggccgg ccgcggcacc gacatccagc tcggtggcaa cgcgcagatg
     8281 cgcatccagc aggagaccgc cggcctcagc gacgaggaaa aggccaagcg catcgaggag
     8341 atcaaggcgg aaatcgagcg caaccgcgag atcgtgctga aggccgagga agtggtcgag
     8401 atcgagccgg cgaagggcaa caagccggcc aagaccatca cgaagcccgg cgggctttac
     8461 gtgatcggca ccgagcggca cgagagccgc cgcatcgaca atcagctccg tggccgttcc
     8521 ggccgtcagg gcgaccctgg ccgttcgaaa ttctacctgt ccctcgagga cgacttgatg
     8581 cgcatcttcg ggtcgaaccg catggacggc gttctgcgca cgcttggcct gaaggaaggc
     8641 gaggcgatca tccatccctg gatgaacaag tcgctcgaga tggcccagcg caaggtcgag
     8701 gcgcgcaact tcgacatccg caagcagatc ctcaagtacg acgacgtgat gaacgatcag
     8761 cgcaaggtca tcttcgacca gcgcatcgac atcatgggca tggacgacgt cagcgagacg
     8821 atcaccgaca tgcgtcacga ggtgatcgac gagctggtca agcggcacat tcccgaaaac
     8881 gcctacgccg agcagtggga tgcggaaggg ctgcagaccg aggtcaagaa catcttcggc
     8941 ctcgacctgc cgatcgtgga gtgggcggcg gaggaaggca ttgccgacca ggagatccgc
     9001 gagcgcctga tcgaggcggc cgacgccaag gctcgacaga aggaggccga catcggcccc
     9061 gagatgttcc gctacatcga gaagatggtc ctgctgcaga cgctcgacca cctgtggcgg
     9121 gagcatctcg tcacgctcga gcacttgcgg caggtgatcg gcttccgcgc cttcggccag
     9181 cgcgacccat tgaacgagta caagggcgag gccttcatcc tgttccagaa catgctggcc
     9241 aagatgcgcg aggcggtgac tggacagctc atgcacgtgg agctggcgac accccaggaa
     9301 cagcagtcga tgttcgagcc cgccgagctg ccgccgatgg aggctcatca catagacccc
     9361 gtctcaggcg tcgacgagct cgcaatggcc gatgcggcac tcgcggcgga agagctggcc
     9421 tatgccaacg gacccgatcg tcgcgcaccc ttgcagagcc ggcgcggcgc ggatcgaatc
     9481 gatcccaagg acccggcgac ctggggcagg gtttcccgca atgcgccgtg cccctgcgga
     9541 tcaggtaaga agtacaagca ctgtcacggg cggcacagtt gaggataagt gtcccagatt
     9601 ccgcagctcg cccgatttcg tcgtcagctc gggagcgggc atccggaatc aaaaggacac
     9661 tgaaagattg agttgctaag gtgacccgcg atcgaggagc tcgctcgctg gcgaagcgcc
     9721 agcatcggcg aactcttcgg tcgtcgtacg acgtactaga agccgcccct ccacacactg
     9781 ctgccatggt gagatcaggt gcggatactc ggactggtcg gagccaccca cgacagcggc
     9841 ctggcgcttc tcgaggacgg tattcccgcg atcgtgctcg aggaagagcg gttcagccgt
     9901 aggaagcgca ccaaaagctt ccccagacgc gccctgagtc tcgccttgga agggtctgct
     9961 cagcgcctgc gcgagatcga cgtgatcacg acgccgtggg acgtttcgcg cctgcggctc
    10021 accttcctgc aagccctggt cggtcatttg ccgtggagcc tcagcctggt ccgccgctcc
    10081 gcgcgctcct tgcaggacaa cgacatcgtc ctgctgaacg catacttcgc ccgcaaattc
    10141 aaacgctact tcggtatacg gaaactaccg ccgatcgtga acgtcggcca tcacgactcc
    10201 catgctgccg tctacttcgt gagcccgttc gacgaggccg ccgtgctcgt gatggacggc
    10261 tacggcgacg atgcctcgac aagcgcatac atcggacaag gtcgcagtct gacgcggctc
    10321 tggcggaccg gcatcttcaa ctccctcggg atggtctaca ccttcgtcac catgcacctc
    10381 ggattcgggt tttccgagga gggcaaggtg atggcgctgg ctgcctacgg cgatgacacc
    10441 tatgtcgacg cttttcgcaa tctcatccgg ccgatgccgg atggcagcta ccaggtgaac
    10501 ttcaaatact tcagctacga tgctttcggt gagcttcgtc ctttcacgcc ttgctttctc
    10561 gaagcgttcg gcccaccccg ccagagcgga gaggagctga acgacaggca ccgcgccatt
    10621 gcgcgcgggc tgcagacgct caccgaggaa atcgtgttgc atatggctcg cgagctgcgt
    10681 cgcaggaccg gttcgcggaa tctggtcctg agcggcggtg ttgcgcttaa ctgcgtcgcc
    10741 aatgctcgtg tcctggagga agcgggcttc gaccgcatct gggttccgcc ttgcgcctcc
    10801 gatacgggcg ccccgctcgg gagtgcgctc tggcactatc accagacgct cggcaacgag
    10861 cgcaggatgg agttgacgca ccccttttac gggccgctct acgatgacag cgatatcgag
    10921 aaggcccttg cggaagcggg gctttcggcc cgccgtatga gcgaacccga actgctcgac
    10981 cgggtggcgc gcgatctggc gtctggcaag atcgtcggct ggttccaggg gcgttccgaa
    11041 atgggtccgc gggcgctcgg taaccggtcg atcctcgccg acccgcgccg gctggagatg
    11101 aaggacatca tcaacgcgcg cgtgaagcac cgcgagccgt tccggccctt cgccccggca
    11161 gtgttgatcg agcgggcctc agagttcttt cacatcgacc agcctgatcc gttcatgacc
    11221 ctcgcgccgc gggtgcgggc tgagaaggca cgcctgatcc cagccgctgt ccacgtcgat
    11281 gggactggcc gcattcagac cgtcgagcgg agcgcaaatc ccaggtacta cggcgtcatc
    11341 gaagccttcg ggaagctgac cggcgtgcct gtgctgctca acacgagctt caaccgccag
    11401 gagcccatcg tcgagacgcc gcagcacgcc gtctcctgct atctccgtac cggcctcgat
    11461 gtgctcgtga tgggcaatta ctacaccacc gaccgcaatc acgcggacac ggtgcccgag
    11521 agatcgatgc tcgccgcttg actattgcgc catcccggcg gccggcgcag gcggagtggt
    11581 tgccggttca gccgagccgg atgtcatctc cgcgggttgc ggcgcacgct ccatcgtttg
    11641 cggctcaatg gtcgtcggct cggttacggc cggcgcgggc ggctccggtt cggtcggtgt
    11701 tgccatggtc gcaatggtcc gcttcgcctt tgcccgcagc acctccagct cgatgcagtc
    11761 cgccagcgtc cgccagctat gatcggccgg gtcgctggtc gctgcgatgc acgcgtcctg
    11821 cacctgggtc ggcaggaccg cccagctgcc tgtaatggca ttacgcgcct gcgcctcgaa
    11881 aatacgacac tggccaggcg cactgtcctg gcggcaaaca tctgtgacgt cccaatccgg
    11941 caaattcgcc gcagaagcag gcatcgcgag aaaagcggcg gcggccgctg cgaggatacg
    12001 tgtgaccatt tcgattcccc ctcaggaata gggatccggc aacggatcgc tcattgcgct
    12061 atgaaccatt tgcactaaat tcgaagggtg cgccatggct caatcataaa gcaagtttcc
    12121 atgtccaatc ttgaccttag tcacgctgac cagcgcgacg gccgcatcag actctacggc
    12181 ccggatgatt tcgcagctat gcgcaaggct ggccggcttg cggctgaggc tctggacatg
    12241 ctcgtccctc acgtgaagcc gggggtgacg acggagtacc tcgacgagct catcttcgag
    12301 ttcgccatgg atcacggggc cattccggcg acgctcaact accggggcta cgagaaatcg
    12361 atctgcacct cggtcaacca cgtcgtctgc cacggtattc ccggccccaa gccgttgcgc
    12421 gaaggagaca tcgtcaatat agacgtcact ctcatcgtgg atggctggta cggcgacacg
    12481 agccgcatgt atcccgtggg cgaaatcagc cggagggccc agcgcctcct cgatgtcacc
    12541 tacgaggcac tgatgcgcgg cattgccgcg gtcaagccgg gtcatacgac gggccacatc
    12601 ggcgcggcga tccagaccta tgccgaggct gagcgctgca gcgtggtcag ggatttctgc
    12661 ggccacggcg tcggccgggt gttccatgac cgcccgaaca tccttcacta tggcaaccca
    12721 ggcgaaggac cggagctcaa gccggggatg attttcacca tcgagcccat gatcaacctc
    12781 ggcaagccgc acgtgaagat cctgtcggac ggctggaccg ccgtaacgcg ggatcgtgag
    12841 ctctccgccc agtacgagca tacggtgggc gtgaccgaga ccgggtgtga ggtctttacc
    12901 tattcgccag cgggcctgca ccagccaggc cgctgaggac ggggccgggt ttgctgaggg
    12961 gggagcaagc ccagtgtcga attccgatga aagcagcttc acgggcaggc cccggcaagg
    13021 cgccttcttc gaggcgccgc taccgagcca tgtcggacac cgccaaagac tgcgcgagcg
    13081 gttcaagaca ggcggccccg acgcgctgcc cgactatgag cttctcgagc tcttactgtt
    13141 ccgcgccatt ccacgccggg acacgaagga gctggccaag cgcctgattg cccgcttcgg
    13201 cagcttcgcc gaagtcatca acgcgccgga gcatcgcctt gcagaggtga aggacgtcgg
    13261 cgaggcggtc atcaccgagc tcaagatcgt cagggccgcc gcgctacgcc tgatgcgcgg
    13321 agagatcatc aagcgcccgg ccctctcctc atggaatcag gtcgtcgcct actgccgcgc
    13381 ggccatggga ttcgaggcgc gggagcagtt ccgcattctc tttctcgaca agcgcaatca
    13441 gctcatcacc gacgaggtcc agcaggaagg caccgtcgac catacgcccg tctatgtgcg
    13501 cgaggtggtg aagcgagcgc tcgagctttc ggcaaccgcc ctggtgctgg tgcacaacca
    13561 cccatccggc gatcccaccc cgtcgcgcgc cgacatcgag atgacgaagc agatcatcga
    13621 ggccgcgcgc cccttgggca tcacgatcca cgatcacatt atcgtggggc gcgagggcta
    13681 cgttagcctt cgagggctgc gccttatatg agcgatgtgg cccgagcagc cgtgtatcag
    13741 aatgagcctg cgggcgcggg ctccgccaag gcaaccgttg acgtggccga aactgcgagt
    13801 cttcatgacc cgccatccaa aggcgggcat ctcaaccgat cagatcgtga gcgcctcatg
    13861 acccagacga agcacgcaag agtcctcatc attggctccg gcccggccgg ctataccgcg
    13921 gccatctatg cggcccgcgc catgctcaag ccggtgctga tccaggggat ccagccgggc
    13981 gggcagctga ccatcacgac cgacgtcgag aactacccgg gatttgccaa gcccatccag
    14041 ggcccctggc tgatggagga gatgaaggcg caggccgaga acgtcggcac cgagatcatc
    14101 atggaccaca tcacgaaagt ggacctgaag cagcgcccgt tccggctcga aggcgattcc
    14161 ggcgacatct acacctgtga cgttctcatc atctgcacgg gtgcgcaggc gcgctggctg
    14221 gggctcccca gcgaagagac ttacaaaggc tacggcgtct ccgcctgcgc cacgtgcgat
    14281 ggctttttct acagggggaa agaggtcatc gtcgtcggcg gcggcaacac cgctgtcgag
    14341 gaggccctgt tcctgaccaa ctttgccaac cgggtcaccg tcgtccaccg gcgggacagc
    14401 ttccgcgcgg aaaagatcct tcaggaacgg ctgttcgcca atccgaagat cgaggtgctg
    14461 tgggacacca ccgtcgagga gatcctcggc acgaccgatc ccaagacggt aaccggcgtg
    14521 cgcctgaaga acgtgaagac cggcacactg agcgagcggc ccgtggacgg ggtattcatc
    14581 gctatcgggc acgcgcctgc gacggagctg ttcaaaaacc agctcgagat gaaaccgtcg
    14641 ggttatatca ttaccaagcc cgattcgacg gccacctcga tccccggcgt gttcgcggcg
    14701 ggcgatgtga aggacgacat cttccggcag gcagtcacag cagccgggat ggggtgtatg
    14761 gctgctctcg aggcggagcg ctacctggcc ggccaagccg ggaccgcctc gatcgccgct
    14821 tggtagccag ctattggcgg agggttgttc gccccctttg tttttgaacc gatttagctt
    14881 aagctgcgcc gctaaacttt tgcaagctgg ccgcgcgtca agcgaagagc catccgtctg
    14941 aagctgcagg aattgaatgc aatgccgccg ctgaccgctg gtcaggttct gccggaacgc
    15001 gctcgcgcga ccgtagccag gaacgtcatg gattgggaca agctgaggat cttccacgct
    15061 gcggccgagg ccggcagttt cacgcatgct ggagaagttc tgcgcatgag ccagtcggcc
    15121 gtgagccgtc aggtctccgc tctcgagcgg gatctgggcg tcgcgctctt ccacaggcat
    15181 gcacgcgggc tcgtgctgac cgagcagggc gagatgcttt accgcaccgc caccgaggtc
    15241 atgaacaaat tgcagacagc cgagatgctc ctgtccgaca cgaccacgaa gcccagcggc
    15301 gatctcagga tcgccgcacc ggtgggcctt ggcacggtct gggtgacgca gcggttgcgg
    15361 gagttcatgg agctctatcc cgacatccgc gtcgaactgg tgctcaacga cgatcaaatc
    15421 gacatctcga tgcgggaggc cgacgtcgcg atctggacgc gcgagccgga gcacgccgac
    15481 ctgatccgcc ggcccttgtt cacgatgagc gtgcgcgcct acgcctcgac ccagtatgtg
    15541 cgcatgcacg gcgcccccca gtccctcgaa gacctcgaaa agcaccgctt cgtctcctac
    15601 agcggcacac ccgcgatgca cttatcagcg atccgggctc tcgagacgat cggccgtgac
    15661 gggcaggagc cgcgcgtgcc ggttttcacg gccaacagca tcgtcgcatt gaagtatgcg
    15721 atccgcgccg gcatcggggt tggattgatt ccggattacc tcacgggcga cgacaccgat
    15781 ctcgttacgg tgctcaatga tgtccagttt ccaaggattc agatcatttt cgtttacccc
    15841 gaggagttgc ggacatcgaa gaagatccag gttttgcgcg attttctggt cagcaaggcc
    15901 aggtctttga aaacctgagc agcttcgaaa caaggtcatg cgggtcccgc atggcaaaca
    15961 tgcgagtagg cagcttgctg atttgcgggt catgctgctt atgtgttgtg cagctgtgac
    16021 ggacagctga tgaaggccgt accctccctt tgacgccttc acgcatcctt ccggatgcgg
    16081 tctcgactag agaaccgggc cccaggtggc ccggtctttt ttcttcaagc tgcgcaaacg
    16141 gatcacagcc actcgggctt ttcagccgcg ctgcgaacgg ctatcagaat gcagcgaagt
    16201 tgggagcaag agacgagaat ggcgcgcacc ctctacgaca agattttcga agaccacatc
    16261 gtcgaccgtc agcctgatgg cacgtgcctg atctacatcg atcgtcacct cgtgcatgag
    16321 gtgacctcgc cgcaggcgtt cgagggtcta cgactggcca accgcaaggt gcgcgcgccg
    16381 gaaaagaccc tggccgtcgt cgatcacaac gtccccacct ccgaccgcag ccgcgggatc
    16441 gacgatcccg agagcaggac gcaggtcgaa gcgctggccc agaatgccaa ggacttcggc
    16501 atcgaatatt acaatgagct ggatgtccgg caggggatcg tgcacgtcat cggcccggag
    16561 cagggcttca ccctgccggg cacgacgatc gtctgcggcg acagccacac gtcgacccac
    16621 ggcgcgttcg gcgcactggc gcacggcatc ggcacctccg aggtcgagca cgtgcttgcc
    16681 acgcaaacgc tcattcagcg caaggctaag aacatgcgca tcaccgtcga cggcaagctg
    16741 ccgaaagggg tgaccgcgaa agacatcatc ctgtcgatca tcggcgagat cggcaccgcc
    16801 ggtgggaccg ggcacgtgat cgagtatgcc ggcgaggcca ttcgcgcgct gtcaatggaa
    16861 ggccgcatga cggtctgcaa catgtcgatc gaggccggtg cccgggccgg catgatcgcg
    16921 ccggacgaga agaccttcga gtacatcaag ggccgtccca aggcgccgaa gggcgcggcg
    16981 tgggacatgg ccatgcgcta ctgggagacg cttcactcgg atgaaggcgc ggtcttcgac
    17041 cgagaagtga agctcgacgc ggcgaacctg ccgccggtgg tcacctgggg tacgagcccc
    17101 gaggacgtgg tctcggtgac aggcgtcgtg cctgatcccg acagcgtcgc cgacgaggcg
    17161 aagcgcgcct cgatgaagcg cgctctcgag tatatgggcc tgacgccggg gaccaagata
    17221 accgacatca agctcgaccg ggtcttcatc ggctcctgca ccaacggccg catcgaggac
    17281 ttgcgtgagg ttgcccgcat cgcccagggc aagaaagtcg cgccgcacgt ctatgcgatg
    17341 atcgtgccag gctccggcct cgtgaagcag caggccgagg ccgaggggct cgacaaaatt
    17401 ctgaaggaag ccggcttcga ctggcgcgag gccggctgct ccatgtgcct cggcatgaat
    17461 ccggaccagc tgaagccgcg cgagcgctgc gcctcgacct cgaaccgcaa cttcgagggc
    17521 cggcagggcc gcctcggtcg tacgcacctc gtgtcgccgg ccatggcggc ggctgcggcc
    17581 atcgccggac acttcgttga tgtccgaacc tggcgttgag tcaactcctt cctgacgccc
    17641 ggtggtttca ccgggcgtat ttgtacaact tcacgtaggc ggtgcgtcgg acatgtctga
    17701 gccgcgatca ctggtccagc agcagttcgg ggcccacgct gcgggctatg tcagctcggt
    17761 cgtgcatgcc aagggcgaaa gcctgcaacg gctcgtcgat ctcaccaggc cggagaagca
    17821 ctggcgggcg ctcgatatcg ccaccggggc tggccacacg gcgctcacgt tcgcatcgca
    17881 ggtcgcccat gtcacagcca gtgacatcac cgacgagatg ctggctgaag ccgaaaagct
    17941 cgccaaatcc cgcggcattg cgaatgtgag ctttgaaaag gcagatgccc agaccttgcc
    18001 gtttccggac ggcggcttcg acctcgtcac cagccgcatc gccccgcacc attttcctga
    18061 cataccgaag ttcgtcgccg aggcgtaccg tgtcctcaag ccgggtggcg tgttcggcct
    18121 cgttgacaac attgcgcccg acgaaaacac gacgccgggc ttcggtgccg aagcgctgcg
    18181 cgcggccgcc gatgcctaca acgacttcga aaagatccgc gatccaagcc atggccgctg
    18241 cctcacgatc ggcgagtggc gcgacgtgac ggtcggcgcg ggcttcgtga tcgagcatct
    18301 cgagaagctg tcgaaatcgc tggagtttgc cccctgggcg gagcggatgg gcgccggtcc
    18361 cgagaccatc gcacgcctgc aagcgatact gaacgacagc tccccggctc tgcgcgcata
    18421 tctgcggcct gagcagcgag acggggcgct ctacttcacg ctcgatgaag taattctggt
    18481 cgcgcgcagg ccggcctgag cccttacggc tttttgggcg gagccagtgg tgcccgctcg
    18541 ccggccgaaa tttcccgcgt gcccaacacg tccgcgagcc ggacctgggc tgagccgggc
    18601 cggagcggct tctgctggct ctcgtgcggc gcccagcccg tcaaggtaat gatctcgaaa
    18661 gttgcaggga cacgaccgtc aggccggccg tgccgttcga tatagatttc gcaggcgcgg
    18721 atgagggtgg agcgccgtaa cggcgtgcgg cggcgggcca ccagcatatt ggtagccccc
    18781 attccacgaa gctcgtgcat cagcgccagc ggcgacttgt acgtcaccgt caccacatcc
    18841 gtatcggtga ccggtagcgc aaagcccgct cgctgcagga ggcttccgaa gtcgcggacg
    18901 tccgcgaacg gggcgatccg cgggctggcg ccgccctcca cttccgcctc ggccagcaaa
    18961 aaggcctcgc gcagctcctg gagggtttga ccgccgagga cggctgccag caacagtcca
    19021 tcaggcttca gcgcgcggcg gatctgagcg agggtcccag gcagatcgtt cacgtactgc
    19081 agcaccaggg ctgagacgac gaggtcgagc gattcgtctg cgaacggcag cgcctcgaaa
    19141 tcgatcagga cccgcggcgg cgggcactgc tcgagcatct gccacgagag ttccgcgctg
    19201 accaggggcg aaacattggg aagctgccga aggctgcggg acagcagccc ggtgtgggcg
    19261 cccaagtcca ccgccgacgc gaactggcgc tgcacggccc cgatccggat aacgaaatcc
    19321 tccgccacgc gctgcagcag aaaatcgtgc tccgccaagt tcggggcggc acgatcccgc
    19381 cggcgcgcca gcaggccaag gtcgaagagc tggacgtcac tggtcatgcc gaccaacgcg
    19441 ccaagagcgg atctgttgaa ctcatctcga taccatctcc ccaaagcggc tggccggtcc
    19501 taggatgacc ggggccgaat tctcatgacc atcctcgagc gcattcgcca ccattcgaag
    19561 cgagcagcca atgcgttcgc cgatatcgtc gtgccgccca cgtgcctcta ttgccgtcgg
    19621 atgctcgcag tgcacgatgc cttatgcgcg cagtgctggc gaagcatcaa gttcatccgc
    19681 ccgccgttat gtgaccggct cggcatccct ttgccctacg acatcggtgg tcgcgccatc
    19741 tcggcccagg cagaggccca tcccccggaa tacgaccgcg cccgcgctgt tgcccacttc
    19801 gatggcgtgc ttcgcgacct catccaccag ttgaagtatg ccgacagcca catcgcccgc
    19861 cggttgtttg gacgctggct cgccgaagcc ggggccgagc tgctgcggga tgcggacctc
    19921 atcgtccccg taccgctcga ccgttggcgt ctactgaagc ggcgcttcaa ccaagcggct
    19981 attttgtcac gggagcttca tcggctcacg ggcactccct gggatccgct cgtgctgttg
    20041 cgctcgaagc gaacagcgac ccaggtgggg ctgacccggg atcagcggcg ccgcaatgtg
    20101 caaggggctt tctcggtcga tcccgcacgg gccaatgcgg ttgagaatcg cgctatcgtc
    20161 ctcgttgacg acgtgatcac gaccggtgcc actgtcgaag cctgcgcgcg ggtgctgaag
    20221 cgagccggcg ccgcgcgcgt cgatgtcctg gccctagggc tcgtcacccc ggatagcatg
    20281 atcacgccgt gaaggactgc ggcgatggtt cctcatagga catcttttac ccgcgacgcc
    20341 cgggcactac cttttgctcg cggctcggac cagtatgcag aaatgtcgaa aatcacgatt
    20401 tacacaacga tcttctgccc ttattgcgtg atggcgaagc agctcctgaa gcgtaagggc
    20461 gtgacattcg aggagatcga cgtcaccggc aaaccggctc tgaggcaaga gatggcggca
    20521 aaggctggcg gccggagtac cgtcccccag atctggatcg gacgtaccca tgttggtggc
    20581 tgcgacgacc tctacgcgct ggaccggacg gggcaactag accggatgct cgcggattag
    20641 ctccaggcag gggggttcga catgcccgcc gcaacgaatg gacgaaaatt ccgggccggc
    20701 atcgtgcaga tgtgcacggg cagagatgtc gcgcgcaatc ttgccgagct gagccggctg
    20761 gtccgtgaag ccgtagcggc gggtgcccag tatgtgcaga cgcccgaagt gtccgtgttg
    20821 atggaacggg atcgcgaccg gctgttcgtg gaaacccggc gcgaggaggg caatccggcc
    20881 atcgcgcact gccaggcgct ggcgcgcgag ctcggcatct ggttgcatct cggatccatg
    20941 ggcatcttgg tgaagccgga caagatcgcc aaccgctcgt tcctcttcac acccgaaggg
    21001 cgaatcgcgg cgcgttacga caagatccac atgttcgatg tcgacctacc gggcggcgag
    21061 agctaccggg aaagccgccg tttcgagcct ggcagccagg ccgtcgtcgc cgatctgccc
    21121 tggggcaagc tcggcctgac gatctgttac gatatgcggt ttccttcact ctatcgggcg
    21181 ctcgccaagg cgggtgcagt tttcattgcg gtaccgtccg ctttcaccgt gcccacgggg
    21241 caggcgcatt ggcatgcatt gctcagggcc cgcgctatcg aggcgcaatg cttcatcttt
    21301 tcagcagctc aggctggaca gcacgaagat ggccgcaaaa cctacggcca tagcctgatc
    21361 gtgtcgccat ggggcgaggt gctcgccgaa gccgatggcg agaacccgtc ggtgatcctt
    21421 gccgacgtcg atgtcgatgc ggtggacgag gcgcgcaggc gtataccctc gctccagcac
    21481 gatcgggcct ttgagatcgt ccaagcccag gctgcagcag attcggatgg agagtcatga
    21541 tccgctacag cttgcagtgc aacaagcagc atacgttcga ggcctggttc aagagcagca
    21601 ccgacttcga tttgcaggcg aggcgcaatc tcgtcacctg cccggagtgc ggctcgacca
    21661 aggtgtcgaa ggcgctcatg gcgcccaatg tctcgacggg ccgctcgaag tccggcgaag
    21721 tcacggtcgc caatccggag gcggccgcag cattggaaaa gaggagagag ctcatggccc
    21781 tgctgagaag gctcagggcc gaggtcgaga agaacgcgga atacgtcgga ccgcgctttg
    21841 ccgaagaagc cctcaagatg cactacgagg aagtggaaaa gcgcggcatc tatggcgaag
    21901 cgacgcccga cgagacacgc gagctgatcg acgaaggcgt cgagttcttc ccattgccac
    21961 ggctgccgga agatcagaac tgacctgatc ttcctcccct gcgtcccccg ggtcagatca
    22021 gcgcctgccc gaccgacacg ccgccgtcga catatagcac ctgcccggtc gtgaatccgg
    22081 cctcctcgga gagaaagtag gcgcatgcgt gggcgatttc ttcaggctgg ccaaagcggc
    22141 ccaccggcac ttggcgcgtg aacgccgtcc gtgcctccga accgaccggg tagcccttgc
    22201 ggatcatatc cgtctcgatc ggtcccggcc cgatgcagtt cgcagtgatg ccgtaggcgg
    22261 cgtactccaa tgcgaccgtc cgtgtcatgc tgacgaccgc tgcctttgag gccccataga
    22321 cgagccggcc atcctttccc aaggctgcac gcgagccgat gttgacgatg cggccgaact
    22381 tcgcctcgcg catggtgggc aggacggctt gcatgcactg catcaccgag cgcacgttca
    22441 cggccatgat ggcatcgaag tcgtcgagcg tcgtcgcttc cgccccggcg atccgggaga
    22501 tgccggcgtt gttgacgagg cgcaggacgc gctttttgcg cgtgatttcc ttcagcaccg
    22561 ctgcagttgc cccggcatcc gccagatcga caggatagaa atcgccctcg aatgcctccg
    22621 gctgcgtgcg cgccagacca acgacgtggt ggcctcgctt cgccagctcc cgcgcgatgg
    22681 ccgtgccgat tccgcggctg acgccggtga cgagagttac cgagccgtgc tcgcctgcgc
    22741 cggaaatgct catgcaagct tcctcccatt tggatgtagg ccgagcttat gtttcccgat
    22801 catcagactg caacttggcc tgccctgcaa caccaccggc agccaatggc gcgatttttc
    22861 gcgccgatct attgattcct gtcgtagatg gtctaagtcg tgcggccggg gtcggcagtg
    22921 gagccggccc aattctcaga gagaagcaag atgggctaca aggtcgctat tgtcggcgcc
    22981 acgggcaacg tgggccgcga gatgctgaat gttctggcgg agcggcaatt tcctgcagac
    23041 gaggtcttcc cgatcgcgtc gcggcgctct atcggcacgg aagtcagcta cggcgagaaa
    23101 acgctcaaat gcttcgacct cgagcagttc gacttcaaga aagcggattt ctgccttatg
    23161 tcggcgggcg ggagcgtctc gaaggagtgg gctccgagga tcgccgcaac cggctgcacg
    23221 gtgatcgata attcctcggc ctggcgttac gaccaggacg tgccgttgat cgtgccggag
    23281 gtgaatgcgg aggcggtgca gaacttccgc aagaagaata tcattgccaa cccgaactgc
    23341 tcgaccgcac agatggtggt cgtcctgaaa ccgctgcacg actacgcgac gatcaagcgc
    23401 gtagtcgtct cgacctacca gtccgtctcg ggcgccggca aagaggccat ggacgagctg
    23461 tggcaccaga ccaagggcaa gtacgtccca ggccaggagg tcgagccgaa gaagttcccg
    23521 aaggagatcg ccttcaacgt cattcctcac atcgacgtct tcatggagga cggctacacg
    23581 aaagaggaat ggaagatgat ggccgagacg aagaagatcc tggatcccaa gatcaaggta
    23641 acggccacgt gcgcacgcgt gcccgtcttc gtcggccact ccgaggctgt gaacatagag
    23701 ttcgagaagc cgattacggt cgaggaagcc cgcaacatac tgcgggaggc gcccggcgtg
    23761 atggtcgtcg acaagcgcga gcctggcggc tacgtcactc cggtcgaagc ggcaggcgag
    23821 tttgccacct tcgtcagccg gatccgcgag gatgccactg tcgagaacgg gctcgccatg
    23881 tggatcgtcg cggacaacct gctcaagggg gccgccctca acacggttca gatcgccgaa
    23941 acgctgatca accgcaacct tctggccaac gcggcttgac gcgccgacac cgcgatcggt
    24001 tcaaatcaac gaggcaggcg attcccatcg cctgcctgtt ttgcacacgc tcctgctacc
    24061 ctcacgccga cttcgcctcc cgccggaggc cctcttcggc ctcgaggaag cgcacgatct
    24121 cggtcaagct cttcaagaag cggtctctct cggcgccgga caacacagaa agcagcctct
    24181 cgtccgcgcg gcgcgcaagc ggctcggcct tcttcaaaac gcgccagcct tcatcggtga
    24241 gccgtaccgc gtaggctctc gcatcctcac gggtgcggcg gcgctggatc aggcccttct
    24301 tgagcatccg ccggatgacg tcggccagcg tcgaccggtc gatgcctgtc cgttcgacca
    24361 gatgcgtctg actaaggcct tcgttctgcg ccaccgtgag cagaattgcg aactgccgcg
    24421 gggtcaggtc cccgtcaatt tctgcctgga agatatcgct ggcgcactgg ctggcgcgat
    24481 gcaggagatg catgggggac tggtcaagcc ggcccattga tgattgtcgc gacatctatc
    24541 ctgtccaggt gttctcaccg ggggtcccgg taagctcatg attcgatttg ccaaccggca
    24601 aatcgaaatg atcacacgaa acgatccgcg tccagattaa gattgttgaa ccagacgcaa
    24661 aaatcaattt taaatcggga tgtggcgttt cagcaggcga aatttgcgag acggtcagct
    24721 ggattggtgc tccgcttgat accgcttgag aatgcccatc tgcgccagag cgaaggccat
    24781 cgtgaggggc atgatccccc aaatcttgaa gctgatccag aagctctcgg agaagtttcg
    24841 ccagacgatc tcgttcaaga ctgccaggaa ggcgaagaaa cacgcccacc gcacggtcag
    24901 gattacccag cctcgatccg taagctgaaa catgtcgccc atcaggggcc gcagcagcgg
    24961 gcgatccaac aataggccgc caaatagaat gattgagaat aaggcgttga caatcgttgg
    25021 cttcagcttg atgaacaccg cgtcatgtag gatcagcgtc agcgcgccga acgcgacgac
    25081 gaagataccg gagacgagcg gcatgaccgg tatacgtccg ag
//
LOCUS       DASVDU010000076        16901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_427244, whole genome shotgun sequence.
ACCESSION   DASVDU010000076 DASVDU010000000
VERSION     DASVDU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427244"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VF226_05010"
     CDS             complement(<1..750)
                     /locus_tag="VF226_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7073380.1"
                     /translation="MVKRVGGALLHSIVIDPRARRPVSTQVYVALRDMILTGAFAAGE
                     RLPATRTLSRELGLSRTTIIEAFERLAAEGLIEARVGSGTFVSALLATDRPKRATPWH
                     TAADAKPARLSKAMTHATTRFSERQRLPFVPRAFTTALPAFDAFPMAQWARLSVKHWR
                     GNRNDVMGYGEPNGHPQLRRAIAAHLRANRGISCDAEQIFIVGGAQQAFHLIGSMLLD
                     PGDKVWFENPGAIGARNSMIACGADLIPVAVD"
     gene            879..1982
                     /locus_tag="VF226_05015"
     CDS             879..1982
                     /locus_tag="VF226_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX7073381.1"
                     /translation="MRIKSIETFCNQFVGFLRVTAETGVQGWGQVSTYNADITAQIVH
                     RQIAPWSLGRSVFEADDLLDRIIEREHKFPGSYICRAMAGLDTALWDLRGKLEGKSVC
                     ELLGGTPRPIRAYASSMKRDITPQDEADRLLRLRDELGFDAFKFRVGAECGHDVDEWP
                     GRTEEIIPYMRRAMGDDVDLLVDANGGFSPKRAIEVGRMLEDNGICHFEEPCLYWELE
                     QTKEVRDALDVDVTGGEQDCWIPTWRHMIEIRAVDIIQPDVCYLGGISRMLRVAEMGA
                     KAGMPCTPHSANLSMVTLFTMHLLGAIPNAGKYLELSIEGPDYYPWQDGLFRSSPYQV
                     SEGKISIPSEPGWGVEIAPDWLERSTYQISEVE"
     gene            2126..3181
                     /locus_tag="VF226_05020"
     CDS             2126..3181
                     /locus_tag="VF226_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073382.1"
                     /translation="MTDLRKLLLLAAISVVSTAGLTTGALSQEITVVSFGGSYQEAQS
                     KALFQPAAKALGIKIKEETYTSIADLRLKVKAGAVTWDVVTSGSGSAARAGAEGILEK
                     LDYKVIDTSDFYPGLAQEYCVGGDVFSTVLAWNTKTYGDNGPKSWADFWDVKKFPGKR
                     AYRKSVAGALEPALMADGVPPEKVYEVLSAPGGIERAINKIKELKPHIAVWWASGAQH
                     AQLMKDGEVDMTTGWNGRFDVAAKDGAKVAYTFNQALLDYDCYAVAKGAPNQDLAMKF
                     IAEISKPQYQAEFTKYITYGPTNKKAYDLGTIDAAYAKKLPSHPDNVAKQLPINLDWY
                     IKHEADASAAYQNMLTE"
     gene            3264..4349
                     /locus_tag="VF226_05025"
     CDS             3264..4349
                     /locus_tag="VF226_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073383.1"
                     /translation="MVRGASLPITIRKLTKSYGEVHALDNVDLDIESGEFLTLLGPSG
                     SGKTTLLMVLAGFTRPDYGSVCFGDREVIRLAPHKRDVGMVFQSYALFPHMDVRANIA
                     FPLKLRGLPRREIADRVDAALEMVQLGGYGERRIDQLSGGQRQRVALARAIVFEPRIV
                     LMDEPLSALDKQLREAMQIELRQLHEKLGTTTIYVTHDQREALTMSDRIAVINQGKVV
                     QLDTPRRVYDRPTSRFVAEFIGESTFLEVECVEGTCRLAGQPLKVEDTPPSSGRLLLM
                     LRPERLQILDSQSDSADNVFEGRVTTVVYQGDTFLLQVVLDNGARLTVRGIASSRSMA
                     RAPQAGDPVRLGLGAEDAVLLPDQPRT"
     gene            4359..5267
                     /locus_tag="VF226_05030"
     CDS             4359..5267
                     /locus_tag="VF226_05030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073384.1"
                     /translation="MSSSDVLIGQGRPNAAGLRRDQLFERWTLLALTAPALLLVLITM
                     IIPVGWLFWLSFLADDGSLSLEHYRRMIEQPSYGRTFLITFEVSLLTTAICIMLGYPL
                     AYVLSQLPRRIANLCMIAVLLPFWTSLLVRTYAWLVLLQRQGLINAWGINLGLWDEPL
                     ALVHNLNGTLIGMVHIMLPFLILPVYGNMRALDRDLLKAAANLGASPTRAFWLVFFPL
                     TLPGLLAGTLIVFILCLGFYVTPAVLGGGKVIMVSNRIANDIELFFNWGAASALGVVL
                     LILTLLFIALAARLVRLDRMLGGGPG"
     gene            5267..6085
                     /locus_tag="VF226_05035"
     CDS             5267..6085
                     /locus_tag="VF226_05035"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073385.1"
                     /translation="MSWLDEPASETQIPHRSRLWLYVLAALVLTFLVVPTLIVVPMSF
                     SGSQYLEFPPRQWSLRWYESYFASSSWMQATVTSFKAGALTSLVATPLGTMAAYGIFL
                     SRSRWTVLLYVILITPIIVPVILVGIAVFYAYVRLSLVNTLIGIVLAHSMLAIPIVML
                     IVTSALKSFDMNQELVARSLGANRLKAFLLVTLPQIRFAVITSAILAFLTSFDEVIVA
                     MFVSGGDNSTLTRNMFTALRDQIDPTIASISTIMIVVSSLLLAVSQLFARSRKD"
     gene            6227..6859
                     /locus_tag="VF226_05040"
     CDS             6227..6859
                     /locus_tag="VF226_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEX7073386.1"
                     /translation="MIDVVTFWLDWFGVAVFATTGALVASRKQMDLIGFILLGTVTGV
                     GGGTIRDTLLGNLPVFWIADPRYLITCVVVSSAAFFLAHIPQSRYALVLRFDAVGLAV
                     FAVTGAETALNANADQIVAVAMGVITATFGGIVRDILGGESPAILRQEIYVTAALVGA
                     TLFVALAEAGVERSGSLIAGFVIALIIRLGALRWNWSLPRYRPRPGQPIQ"
     gene            complement(7344..7916)
                     /locus_tag="VF226_05045"
     CDS             complement(7344..7916)
                     /locus_tag="VF226_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX7073387.1"
                     /translation="MATVIAGFLASPAFAHCDSVDGPVAGAAIEALETGNVNLALPYA
                     PASAEDEIKAAHQMAWKARVDGKAAAEVADRWFMETVVRLHREGEGATFTGLKPAGIN
                     YGPVIPAAEKALETGDPTQVKTLLSGAVERLLEERFKDAQKVLESTRKTPPQTAVEVA
                     EARERVNAEFAFIEYAEGIHAALKAEGHAH"
     gene            8048..8215
                     /locus_tag="VF226_05050"
     CDS             8048..8215
                     /locus_tag="VF226_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7073388.1"
                     /translation="MNAQHANWDDLRLFLAVARAGSLSGATRALGTSRPSARPIRASS
                     SRSSPPTPRSA"
     gene            8367..8711
                     /locus_tag="VF226_05055"
     CDS             8367..8711
                     /locus_tag="VF226_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7073389.1"
                     /translation="MGFDSDHEALVRRFANFLPETRPIFRANSVAVAIAAAKAGMGLA
                     PLPCGIADLEPDLVRIAPLPDDFTLDLWLLTHEDLRRTARIRAFLDFLAAALAAEAPL
                     LEGQKPRARSNG"
     gene            8849..12091
                     /locus_tag="VF226_05060"
     CDS             8849..12091
                     /locus_tag="VF226_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7073390.1"
                     /translation="MRDLTAWLAQIGLEKYASAFVAHEVDLAALRHLTDDDLKNIGLP
                     LGARRKVLAALAASDVRQEGTTKPAPERRPVCVLFADLVGYTKLSQRLDAEDLHQALE
                     GFFGCVDRIVQQHGGRVDKHIGDCVMAAFGAPVAHGNDVERAVAAALAIRDAVPDLGA
                     AFGPLLAVHIGVASGQVVASGVGSSYHREYTVTGEAVNLAARLTNAAGAGEVLVSDRV
                     WRDLAERLDGTDAGLLEVEGFHEPVRAWRAQRLRSAAARRALVGRNAELAQFHSILNT
                     CRQTGRGRAVHLRGEAGIGKTRLLEEVLTIAARQGFACHSGLVLSFGSGTGRDAVKAL
                     VRGLLGLDSAADVGRLEEGAAAAAAAALVDPENLVFLHDLLGLAPSGQLRVLYEAMDN
                     AAREAGRAATLVQLVRRTNRRQPRVLAIEDLHWADAATLDLLAPLAAAVVDCPALLIM
                     TSRIEQNPLDEAWRAKAGGGPLLTIDIGPLHADEAGELAASFPAVDAALARSCIERAA
                     GNPLYLEQLLRYAVEGDASKVPGTVQSLVQARLDRLDADDRLALQAASVLGQRFDREA
                     LAAVLDQPSYDSERFAAHHMVQPHGTQFLFAHALIHEAVYDSLLKSRRAELHRRAADH
                     YAAFDPGLRAAHLDRAGDPQAARAYLAAAQREATEYHYAAAQALGRRGLELANEREDR
                     FALASLLGEVLHDLGDMPAALASCQEALTAAGSDAERCRAWLGLAAVKRVTDDIDGAL
                     ADLIDAEAAAVTHGLTHELARLHLLRGNLYFPSGDIERCLKEHGRSLELAERTGSAEL
                     EAAALGGLGDGEYMRGRMMTARDRFARCVRLCQDHGLVRIEVANRPMLAFAGWFAGDG
                     AKAAAEAALAIEMAQSVAHHRAEMIGRHAACFISLALMNLDAAFEHAEAALALARGLR
                     ARRFEAEGLALRADVRRARGDLPEAARDAEAAVALSFETGPAFVGPFALGVLARTVTD
                     QRRRQEAMAEADRLLSEGAVSHNHLLFRRDAIEMAIDADDWDSAEKYAAALEAYTRDE
                     PLPWAEFYVRRARWLSTIAREGSSPEALAQLRELRAEGERRGLLHALARI"
     gene            12263..12922
                     /locus_tag="VF226_05065"
     CDS             12263..12922
                     /locus_tag="VF226_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7073391.1"
                     /translation="MSWNRDDHRRRRGYHHGNLREALIQAALELISNKGPAGFTFAEA
                     ARYAGVSPAAPYRHYRDRDALMADVARRGFEQFAAALTKAWDEGRPTPSAAFERLGRA
                     YLDFARREPAYFSAMFESGLDLKSFPAVQEAADSAFAVLRGACEVLVSTLPPARRPPA
                     LMMALHVWSLAHGIASLFGRGDAARRPIPMTPEELLEAAMLVYMQGLGFGPAESGSAQ
                     G"
     gene            13083..13529
                     /locus_tag="VF226_05070"
     CDS             13083..13529
                     /locus_tag="VF226_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEX7073392.1"
                     /translation="MRTKAMAEVIERLDDWGKPAWIAVMVLGFAIFWPVGLAILAYLI
                     WSGRMGCGRNGDLARWQSRMAERWERKREKWGLHGRVYSSSGNSAFDEYREETLRRLE
                     EEQREFRSFLDRLRMAKDKAEFDQFMAERRNRSRDAGPGEPGPEQR"
     gene            complement(13697..15286)
                     /locus_tag="VF226_05075"
     CDS             complement(13697..15286)
                     /locus_tag="VF226_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX7073393.1"
                     /translation="MLQTPRATRGMVVAPHHLAAEAGLRVLREGGNAIEAMIAALATI
                     AVVYPHMNGLGGDNFWLIRADDGPPVGIDACGAAWSGADAEFYRARGMNSIPNRGPLA
                     AITVAGAVSGWQAAVELAGGMGGRMPLARLFEDAIFYARAGYPVTRSQHANTAAKRDE
                     LEPVPGFATHYLSVGAVPAVGEIFRQPTLATTLERLAAAGLDDFYRGELAEAIAFDLE
                     AAGSPIRAQDLAAQRARLVKPLSLHAFGATIYNMPPPTQGLAALMILGLFERLGCREA
                     EGFDFVHGLVEATKRAFRVRDAHISDPAYMDVDCDDFLTPDVLDTLAAEIDPCTALRW
                     AGGHTNGDTVWLGVVDGEGRAVSCIQSIYWEFGSGLVLPATGITWQNRGTVFELAPGA
                     RNALKPARKPLHTIQPPLAVFDDGRVMVYGTMGGDGQPQTQAAIFTRYSAFGQELQQA
                     VTAPRWLLGRTWGEQRMSLRIESRFAGETLDALARAGHDLEPIGPFDEVTGHAGAIVR
                     TPIGVLEGAVDPRGDGVVAAF"
     gene            complement(15320..16642)
                     /locus_tag="VF226_05080"
     CDS             complement(15320..16642)
                     /locus_tag="VF226_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7073394.1"
                     /translation="MEGETLALLMCASFIGLLFVGVPVAIALLISGFTFGFLGFGLTL
                     FNLIPLRIYGVATNYTLLAVPLFVFMGVMLEQSRLAEDLLDVIGFLCGPLPGGMGLAI
                     VLVGVLLGAATGIVGATVVTLGLLTLPVFLKRNYSKPLACGVICASGTLGQIIPPSLV
                     LILLADIMAESVGTIFAAAMIPGLVLAGIYLVYVFVLGLLRPELAPPIPKAERDLKTS
                     RQLVVDVFKVVLPPIGLILAVLGSIIGGIAAPTEAASMGAAGALLIAALGRRLDWPTL
                     VGTLRNTLNISAMIYFILIASQAFSLAFRGLGGDGLVEAMFEAVPGGTTGALIFMLVI
                     LFFLGFFLEWIEISYIVMPLFVPIFRNAGIDMVWMSILVGMTLQTTFLTPPVGWSLFF
                     LKGVAPPSITTRHIYVGVAPFVTLQVVAVVLVFIYPDIALWLPRAIGW"
     gene            complement(16646..>16901)
                     /locus_tag="VF226_05085"
     CDS             complement(16646..>16901)
                     /locus_tag="VF226_05085"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7073395.1"
                     /translation="GFGARTRAVVNLFSSIGLLVTSVLVVWLSLNFVNQAYVIDEGSP
                     DPGGLPHRFILKSMIPLGFGLLALQAVAHIILYSKRALGR"
BASE COUNT         3000 a         5362 c         5437 g         3102 t
ORIGIN      
        1 gtcgacggcg accggaatga gatcggcccc acaggcgatc atgctgttgc gcgcgccaat
       61 ggcgccagga ttttcgaacc aaaccttgtc gccgggatcg agcagcatcg agccgatgag
      121 gtgaaaggct tgttgcgcgc caccgacaat gaagatctgc tccgcgtcgc acgaaatgcc
      181 ccgattggcc cgcaggtgag cggcgatcgc gcgccgcagc tgcggatggc cgttgggttc
      241 cccgtatccc atgacgtcgt tgcggttgcc gcgccaatgc ttgacggaaa ggcgcgccca
      301 ctgcgccatc gggaatgcat caaaggcagg aagcgccgtg gtgaaagcgc gagggacgaa
      361 gggcaaccgt tgccgttcgc tgaagcgggt ggtggcgtgg gtcatcgcct ttgaaagccg
      421 cgcaggcttc gcatcggcgg cggtatgcca tggggtggca cgtttcggcc gatcagttgc
      481 gagaagtgcg ctgacgaacg tacccgatcc gacccgcgcc tcgatgagac cctctgcggc
      541 caaccgttcg aaggcctcga taatggtggt gcgcgacagc ccgagctcgc gtgacaaggt
      601 tcgtgtagcg ggcaatctct ctccggcggc gaacgctccc gtcagaatca tgtcgcgcaa
      661 ggcgacgtag acctgcgtgc tcaccgggcg gcgggcacga ggatcgatga cgatagaatg
      721 caacagcgct ccgccgacac gcttgaccat tggctggctc ccggggcgaa ctttgataag
      781 cggtatcaca gaatatcgag aatggatcta tcgatgtgat ccattcagct ggaatgttcc
      841 cgcccgagga aaccaccagt gcccgggcgg gatgacccat gcggatcaag agcattgaga
      901 cgttttgcaa ccagttcgtc ggctttctgc gcgtcacggc tgagactggc gtgcaaggct
      961 ggggccaagt ctcaacctac aacgcggata tcacggcaca gatcgtgcac cgccagattg
     1021 ccccttggtc tctcggtcgc agcgtattcg aggcggatga tctcctggat cggatcatcg
     1081 agcgcgagca caagtttccg ggatcctata tctgtcgggc catggcgggg ctcgatacgg
     1141 cgctttggga cctgcgcggc aagctcgagg ggaaaagcgt ctgcgagttg ctcggtggca
     1201 cgccccggcc gatccgtgcc tatgcgtcct cgatgaagcg cgacatcacc ccccaggatg
     1261 aagccgacag gctgctgcgg ctgcgcgatg agctcgggtt cgatgcgttt aagtttcgcg
     1321 ttggagccga atgcggacac gacgtcgacg agtggcccgg ccgtaccgag gagatcatcc
     1381 cttacatgcg gcgcgccatg ggcgatgacg tggacctgct cgtcgatgcc aacggcggat
     1441 tctcaccgaa gcgggcaatc gaggtcgggc ggatgcttga ggacaacggc atttgtcact
     1501 tcgaggaacc ctgcctctac tgggagctgg agcagacgaa ggaagtgcgg gacgcgctcg
     1561 acgtcgatgt caccgggggt gagcaggact gctggatccc gacttggcga cacatgatcg
     1621 aaatacgggc tgtcgatatc attcagcccg atgtttgtta cctcggaggc atcagccgga
     1681 tgctgcgcgt cgccgaaatg ggtgccaagg ccggaatgcc gtgcaccccg cacagcgcca
     1741 atctctcgat ggtgacgctg ttcaccatgc atctcctggg cgccattccg aatgctggca
     1801 agtaccttga gctgtcgatc gaaggccccg attattatcc ttggcaggac ggtctgttcc
     1861 ggagctcacc ctatcaggtt tcggagggga agatcagcat tcccagcgag cctggctggg
     1921 gcgtcgaaat tgcgccggac tggctggagc gatcgaccta ccagatcagc gaggtcgaat
     1981 agactccacg tcgaaacgta attggtatcg agatctatac cagagtggcc cttttcttcg
     2041 gaccattgtt ggaaccaaac tatccagcgg aggcggacaa cacgaacaag agccgttgca
     2101 aacggatcaa acagggagtg gaacgatgac tgatttgcgc aagttgcttt tgcttgctgc
     2161 catttccgtg gtgtcgacag cgggactgac cacgggggca ctgagccagg aaatcactgt
     2221 cgtgtccttc ggcggatcct atcaggaggc gcagagcaag gccctgtttc agccggccgc
     2281 gaaggctctc ggcataaaga tcaaggagga gacctacacc agcatcgccg atctccgcct
     2341 gaaggtgaag gctggagccg tgacctggga tgtcgttacc agcggctcgg gtagcgctgc
     2401 acgggcgggc gccgagggta ttctcgaaaa gctcgactac aaggtgatcg atacgtcgga
     2461 tttttaccct ggtttggcgc aggagtattg cgttggcgga gacgtcttct ccaccgtgct
     2521 cgcctggaac acgaaaacct atggcgacaa cggtccgaag agttgggccg atttctggga
     2581 cgtgaagaag tttccgggca aacgggccta tcgcaagtct gtcgctgggg cgctcgagcc
     2641 ggcgctcatg gcggatggcg ttcctccaga gaaggtctat gaggtgctct ccgccccggg
     2701 cgggatcgag cgtgcaatca acaagatcaa ggagctgaag ccgcacattg cggtctggtg
     2761 ggcgtccggc gcgcagcacg cccagcttat gaaggacggc gaggtcgata tgaccaccgg
     2821 ctggaacggc cggttcgatg tggctgccaa ggacggtgcc aaggtggcct acaccttcaa
     2881 ccaggcgctg ctcgactatg actgctatgc ggtggcaaag ggtgcgccga accaggactt
     2941 ggcgatgaag tttattgccg agatcagcaa gccgcagtat caggccgaat tcaccaagta
     3001 catcacctac ggcccgacca ataagaaggc atacgatctc ggcacaatcg atgcggcgta
     3061 cgccaagaag ctgccgtccc atcccgacaa cgtcgccaag cagctgccga tcaacctcga
     3121 ttggtacatc aagcacgagg cggacgcgtc tgctgcctat cagaacatgt tgacggaata
     3181 gcagcctgct gcctcaatcg aagttgggcg gtacgcgtcc cgtgccgccc gcctgtcgtt
     3241 caactcaagg ccaacggtct cgcatggtgc gcggcgcctc gctccctatc acgatacgga
     3301 agctgacgaa atcttatggt gaggtgcacg cgctcgacaa cgtcgatctc gatatcgaga
     3361 gcggcgagtt cctgacactc ctcgggccat ccggctccgg aaagacgacg ctgctgatgg
     3421 tgctggcagg cttcacgcga ccggattacg gcagcgtgtg tttcggcgat cgggaagtga
     3481 tccgcctggc cccgcataag cgcgacgtcg ggatggtctt ccagagctat gcgctgtttc
     3541 cgcatatgga tgtgcgcgcg aacattgcat ttccgctgaa gctacgtggc ctgcctaggc
     3601 gggagattgc cgatcgcgtc gacgcagcgc tcgaaatggt ccagcttggc ggctatggcg
     3661 aacggcgtat cgaccagctt tctgggggcc agcgccaacg cgtggctctc gcccgtgcca
     3721 tcgtcttcga gccacgaatc gttctcatgg acgagccgct gtctgctctc gacaagcagc
     3781 tgcgagaggc catgcagatc gagttgcgcc agcttcacga gaaactcgga acgacaacga
     3841 tctacgtgac gcacgaccag cgcgaggctc tgacgatgtc ggatcggatc gccgtcatca
     3901 accagggcaa ggtggtgcag ctggatacgc cgcgccgggt ctacgatcgg ccgaccagtc
     3961 ggttcgtggc ggaatttatc ggcgagtcga cctttcttga ggtcgaatgc gtcgagggca
     4021 cctgccgtct cgccggtcag ccgctgaagg tcgaggatac cccgcccagc tccggtcgct
     4081 tgctactgat gctgcggccg gaacggcttc aaatcctcga tagccaatcg gattccgccg
     4141 acaacgtgtt cgagggccgt gtcacgacgg tggtttacca gggcgacacc ttcctgctgc
     4201 aggtcgtgct cgataacggc gcgcgtttga ctgtccgggg tattgcgagc agccgctcga
     4261 tggcgcgagc gccgcaggcg ggtgacccgg tgcgcctggg cctcggcgcc gaggatgcgg
     4321 tgttgctgcc cgaccagccg aggacctgag agttggccgt gagctccagc gacgtgttga
     4381 tcggacaagg gcggcccaac gctgcgggcc tgcgccggga ccagctattc gagcggtgga
     4441 ctttacttgc gctcaccgcg ccggcgctcc tgctggtgct gatcacgatg atcatccccg
     4501 tcggctggct gttctggctg tcattcctcg ccgacgacgg cagtctgagc cttgagcact
     4561 accgccgcat gatcgagcag ccgtcctacg gccgcacatt cctcatcacg tttgaggtca
     4621 gcctgctgac gaccgccatt tgcatcatgc tcggctatcc cctggcatac gtgctctcgc
     4681 aattgccgcg acggatcgcc aatctgtgca tgattgccgt gctgctgccg ttctggacat
     4741 cgctgctggt gcgcacctat gcgtggctcg tgctgctgca gcgccagggg ctcatcaacg
     4801 cctggggcat caatctcggg ctgtgggatg agccgctcgc gcttgtccat aatctcaatg
     4861 gcacgctgat cgggatggtg cacatcatgc tgccgttcct gatcttgccg gtctacggca
     4921 acatgcgcgc gctcgaccgg gacctcctta aggccgctgc caacctcggc gccagcccga
     4981 cgcgggcatt ctggttggtc ttcttccccc tgacgctgcc agggctgctg gccggcactc
     5041 tcattgtctt catcctttgt ttgggcttct acgtcacgcc cgccgtgctc ggtggcggca
     5101 aggtgatcat ggtctcgaac cgcatcgcca atgacatcga gctattcttc aactggggtg
     5161 ccgccagtgc gctcggcgtc gtgctgctca tattgaccct tttgtttatc gccctcgccg
     5221 ctcgcctggt acggctggac aggatgcttg ggggaggccc cggctgatga gctggctcga
     5281 tgaacccgca tccgaaaccc agatcccgca ccggtcgcgg ctgtggctct atgttcttgc
     5341 cgcgctcgtc ctaaccttcc tggtcgtccc gacgctgatc gtcgtgccga tgtcgttttc
     5401 tggctcgcag tacctggaat ttcctccgcg ccagtggtcg ctccgctggt atgagtctta
     5461 ctttgcgtcg agttcctgga tgcaggcgac cgttacctcg ttcaaggccg gtgcgctcac
     5521 cagcctcgtg gcgacgccgc tggggacgat ggcggcatac gggatctttc tctcccgttc
     5581 gcgctggacc gtgctgcttt acgtcattct catcaccccc attatcgtgc cggtgatcct
     5641 cgtcggcatc gccgtcttct acgcatacgt tcggttgagc ctcgtcaata cgctgatcgg
     5701 catcgttctc gcccacagca tgctcgctat cccaatcgtg atgctgatcg tgacctcggc
     5761 gctcaagagc ttcgacatga accaggagct cgtcgcccgc agccttggtg cgaaccggct
     5821 caaggccttc ctgttggtga ccttgcctca gatccgcttt gccgtgatca cgagcgccat
     5881 cctcgcattc ctgacctcgt tcgacgaggt gatcgtcgcc atgttcgtct cgggcggcga
     5941 caattccacg ctcacccgca acatgttcac cgcgctccgt gatcagatcg atccgaccat
     6001 agcctcgatc tcgaccatca tgatcgtagt gagctctctg ttactcgccg tttcccaact
     6061 cttcgcgcgg tcaaggaagg actagcgcat cgacgcagtc acaataaaaa ttccccgaac
     6121 ttggctcaag atctcctgct cagcacccaa cagacttggg catccgagga gctgttgccc
     6181 gggcacctga tgacccgcta tcgcctctat ccactaaggc agacatatga tcgacgtcgt
     6241 cacattctgg cttgattggt tcggcgtcgc cgttttcgcg acgacgggcg cccttgtcgc
     6301 ctcgcgcaag cagatggatc tcatcggctt cattctgctc ggcacggtca cgggggtcgg
     6361 gggcggcacg atccgcgaca cgctgctcgg aaatttgccg gtcttctgga tcgccgatcc
     6421 gcgctacctg atcacgtgcg ttgtcgtatc cagcgctgca ttctttctgg cccacatccc
     6481 gcaatcccgc tacgcgctgg tgctccgctt cgatgcggtg gggcttgcgg tcttcgcggt
     6541 cacgggtgcc gaaacggctc tcaacgcaaa cgccgatcag atcgttgccg tagcaatggg
     6601 cgtgatcacc gccacgttcg gcggcatcgt tcgcgacatc ctcggtggtg aaagcccggc
     6661 gatcttgcga caagagatct acgttacggc ggctttggtg ggagccacgc tgttcgtggc
     6721 gctcgcagaa gcgggggtgg agcggagcgg cagccttatc gccgggttcg ttatcgccct
     6781 gatcatccgc ttgggcgccc tgcgctggaa ttggtcgcta ccgagatacc ggccgcgacc
     6841 gggccagccg atacagtgac gttcacatag ccgcacagcg ctgagcctcg aaagccccgc
     6901 gccttggcgc gggactcgta acaccagcag gcgctcacct tcaggctgtg ctatcttagg
     6961 ctgcgcaacg cccgcttccg gtactgccgg acgcggcaca gtgccctttg gcgtcgccgg
     7021 cacacgcagc aagcgcatgc cgttggcgat gacgatcaga accgagagct ggtgcatcag
     7081 catgccgccc gccctgtgca catggccgct gaagaccccg gcgagtcagc agggcgatga
     7141 cgaggttctg gcgcatgttg ccgagcggaa ccgcgatagt tctggtcggc ctgaagacga
     7201 cagatgcggg ccgtggttac gggagggccg ccggatcggc aaaacgaagc acggctccgc
     7261 gcctacaaag attgacgacg gagcgccagg atgctccgtc gtcgggaaat gctgaaattg
     7321 tcagtcagct ttcgctccgc cgctcagtgg gcgtgacctt cggccttgag ggcagcatgg
     7381 atgccttccg catactcgat gaaggcgaac tcagcattca cccgctcccg ggcctcggcg
     7441 acctcgactg cggtttgggg cggagttttc cgcgtgctct caagcacctt ctgcgcatcc
     7501 ttgaaccgtt cctcgagcaa gcgctcgacg gcgccgctga gcagtgtttt cacctgcgtc
     7561 ggatcgcccg tttcgagcgc cttctcggcg gccggaatga ccggcccata attgatcccc
     7621 gccggcttca gcccggtaaa ggttgcgcct tctccttccc ggtgtaggcg gaccaccgtc
     7681 tccatgaacc agcggtcggc gacctccgcc gcggcttttc cgtccactcg cgccttccag
     7741 gccatctggt gggctgcctt gatctcatcc tccgccgaag ccggcgcata agggagcgcc
     7801 aagttcacgt tgccggtctc gagcgcttcg atcgcggcgc ctgctaccgg cccgtcgacg
     7861 ctgtcgcagt gggcgaaagc gggactggcg agaaagccag cgattacggt agccatcact
     7921 aatctgcgca taacaatgct cctcagcttt tgcgttttgg gtccggccca attggacctc
     7981 gccttggcag gtagctaaga ctttcgttgc ggcgctggga ttagcgaagg tcgcaaggtg
     8041 gatttggatg aatgcacagc atgccaactg ggacgacctc cggctgtttc tcgcggtggc
     8101 gcgcgccggc tcgctctcgg gggccacgcg ggcgctcggc acctcgcggc cttccgcacg
     8161 gcctatccgg gcatcgagct cgaggtcgtc gccaccaacg ccgcggtcag cctgagccgg
     8221 cgcgaagccg acgtcccgct ccgcgtgagc aatcagccgc ttgagcgctg gtcggacgcc
     8281 gcgtcggccg cctcgcattc gccgtctacg gtgctgccgc cttcacggcc gggaccgaaa
     8341 acaccccgtt agcggaacag gactggatcg ggttcgactc cgaccacgag gccctcgtcc
     8401 gccgctttgc caacttcctc ccagagaccc ggccgatctt ccgcgcgaac tcggtcgccg
     8461 tggccatagc agcggccaag gccggcatgg gcctcgcccc gctaccctgc ggcatcgccg
     8521 acctcgagcc cgacctcgtg cgcattgcgc ccctgccgga cgacttcacg ctcgacctct
     8581 ggcttctgac ccacgaggat ctgcggcgga cggcacgcat ccgcgccttc ctcgacttcc
     8641 tcgccgccgc gcttgcggcc gaggcgccgt tgctggaagg gcaaaagccg cgtgcccgga
     8701 gcaacggata aggggcaacg cgcgttggta gagcaagctg cgtagccccg agcagcgccg
     8761 cgcactcggt gtccgacacc gcggctgatc gtcaggtttg accttttgaa gctcaatgcg
     8821 cgcaaggcac cgaggaaagg ggagatccat gcgcgatctc accgcttggc tcgcccaaat
     8881 tggcctggag aagtacgcgt cagcattcgt ggcgcacgag gtcgatctgg cagccctgcg
     8941 gcacctaacc gatgacgacc tcaagaatat cggcttgccg ctcggcgctc gccgcaaggt
     9001 cctggccgcc ttggccgcct ccgatgtacg gcaggaggga accacgaagc cggccccgga
     9061 gcggcgcccg gtctgcgtgc tgttcgccga tctcgtcggc tacaccaagc tgagccagag
     9121 gctcgatgca gaggacttgc atcaagcgtt ggaaggcttt ttcggctgcg tcgatcgcat
     9181 tgtgcaacag catggcgggc gggtcgacaa gcatatcggc gactgcgtca tggccgcgtt
     9241 cggtgcgccg gtcgcgcacg gcaacgatgt cgagcgcgcg gtggccgccg ctctggccat
     9301 acgagatgcc gtgccggatc tcggggcggc ctttggtcca ctgcttgccg tgcacattgg
     9361 cgtggcgagc gggcaggtcg tggcgagcgg cgtaggcagc tcctatcacc gcgagtacac
     9421 ggtaaccggc gaggccgtga atctggcggc ccggctgact aacgctgctg gcgccggcga
     9481 ggtgctcgtt tcggatcgcg tttggcgtga ccttgcagag cggctcgatg gcaccgacgc
     9541 cggcctgctc gaggtcgagg gctttcatga gcctgtccgt gcctggcgcg cccagcggct
     9601 gcggagcgcc gcggcacgtc gcgccctcgt tggccgcaac gccgagctgg cccagttcca
     9661 cagcattctc aacacctgcc ggcaaaccgg ccgaggccgc gcggtgcacc tgcgcggcga
     9721 agctgggatc ggcaagacac ggctgctcga ggaggtcctg acgattgccg cgcggcaggg
     9781 gttcgcctgc cattcggggc tggtgctcag tttcggcagc ggtaccggcc gcgatgccgt
     9841 caaggccctc gttcgcggcc tgctcgggct ggactccgcg gcggatgttg ggcggctgga
     9901 ggaaggtgcc gcagccgcag cggcggccgc gctcgtcgac ccggagaacc tggtctttct
     9961 gcacgacctt ctggggctcg cgccttcggg acagctgcgc gtgttgtatg aggcgatgga
    10021 caatgcagcg cgcgaggccg gcagggcggc aacgctggtt cagctcgtgc gacgcacgaa
    10081 ccgacgccag ccgcgcgttc tggcgatcga ggatctgcac tgggccgatg cggcaacgct
    10141 tgacctgctg gcgccgcttg ccgctgcggt cgtcgactgc cccgcgctct tgatcatgac
    10201 gtcgcgcatc gagcagaatc ccttggacga ggcatggcgc gccaaagctg gcggcggtcc
    10261 gctgctcacc atcgatattg gcccgttgca cgcagatgag gccggcgaac tggccgcctc
    10321 gttcccggcc gtcgatgccg ccctggcccg ctcctgcatc gaacgggccg ccggcaatcc
    10381 actctatctg gagcagctgc tgcgctatgc ggttgagggc gacgcctcga aggtcccggg
    10441 gacggtccag agcctggtgc aggcccgact cgaccggctc gatgcggacg accgccttgc
    10501 attgcaggcc gcctcggttc tcggccagcg ctttgaccgc gaggcgctgg ctgcggtgct
    10561 ggaccagccc tcctacgata gtgagcggtt tgccgctcat cacatggtcc aaccgcatgg
    10621 gacgcagttt ctgtttgcgc atgcgctcat tcacgaagcc gtctacgaca gcctgctgaa
    10681 gagccgtcgc gccgagctgc accggcgggc tgcagaccac tacgccgcgt tcgatccggg
    10741 gctgagagcg gcccatctcg atcgggccgg ggatccccaa gccgcacgcg cctacttggc
    10801 ggccgctcag cgagaggcaa cagaatacca ttacgcggca gcacaggccc tcgggcggcg
    10861 tggccttgag ctggcgaacg agcgcgagga tcgttttgcg ctggcgagcc tcctcggcga
    10921 ggttctgcac gatctcggcg acatgccggc ggcgctggcg tcctgccagg aggctctgac
    10981 tgcagccggc agcgatgccg agcgctgccg cgcctggctc ggcttggccg ccgtcaagcg
    11041 ggtgacggat gatatcgacg gtgcgctcgc agacctaatt gatgccgaag ccgccgccgt
    11101 gacgcacggc ctgacgcatg aattggcgcg cctgcatctg ctgcgcggca acctctactt
    11161 tccaagcggt gacatcgaga gatgcctcaa agagcacggc cgcagcctgg agctggcaga
    11221 gcgcacgggc tctgccgagc tcgaggctgc cgccctaggt gggttgggcg acggcgaata
    11281 catgcgcggt cgtatgatga ctgcccgcga ccgcttcgcg cgctgcgtca ggctctgcca
    11341 ggatcatgga ctggtgcgaa tcgaggtggc caatcgaccg atgctggcgt ttgcgggctg
    11401 gtttgcgggc gacggcgcaa aggctgccgc cgaggcagcc ctcgcaatcg agatggcaca
    11461 aagcgtggcc caccacaggg cggagatgat cggccgacat gccgcctgct ttatcagcct
    11521 tgcccttatg aacctcgatg ccgcctttga acacgccgag gctgccttgg cactggcccg
    11581 agggcttcgc gcgcgccgct tcgaggccga gggcctggca ctgcgggcag acgtgcggcg
    11641 agccagaggt gatcttccag aggcggcgcg tgatgcggag gcggccgtcg cgctcagctt
    11701 cgagaccggc cccgcgttcg tgggtccctt tgcgcttgga gtcctcgccc ggaccgtaac
    11761 ggatcagcgg cgccggcaag aggcgatggc tgaggccgac cggttgttga gcgagggggc
    11821 cgtaagccac aaccatctgc tctttcggcg cgatgcgatc gagatggcga ttgatgccga
    11881 cgactgggac agtgcggaga agtacgcagc ggcgctcgag gcctacacgc gggacgagcc
    11941 gttgccgtgg gccgagttct acgtccggcg cgcccgctgg ctgtcgacca tcgcgcgcga
    12001 ggggtccagc ccggaggcgc tggctcagct ccgtgagttg agggccgagg gcgagcgccg
    12061 aggacttctt cacgccctgg cgcgcatctg atcacgtggg ctggcgcgcc cgactgtcgg
    12121 ctcacggttc tttttcaggt aaggaatgcg ttttgacgcc tcgcctcgcc gccgtgcttc
    12181 gttcggtgtc cgctgtccgg ggcccttcgc cttgccattc agccctatgt ctcgtggcgg
    12241 atatctgttg tagaaccggc gcatgagctg gaacagggac gatcatcggc gccgtcgcgg
    12301 ctaccatcac ggcaatctac gcgaagccct catccaagcg gcgctcgagc tgatctcgaa
    12361 caagggcccg gccggtttca ccttcgctga ggcagcgcgt tacgcaggcg tcagtccagc
    12421 tgcgccctat cgtcactatc gcgaccgcga tgcgctcatg gctgatgtcg ctcgccgcgg
    12481 cttcgagcag ttcgcagcgg ctctgaccaa ggcgtgggac gagggacggc ccacgccatc
    12541 cgcagcattc gaacgactgg gccgcgccta tctcgacttc gctcgcagag aacccgccta
    12601 tttctctgcg atgttcgagt ccgggctcga cttgaagagc tttccggcag ttcaggaggc
    12661 tgcagatagc gccttcgccg ttctgcgcgg cgcctgcgag gtgctggtgt cgacgctgcc
    12721 gcccgcccgg cggccgcccg cactgatgat ggcactgcac gtctggtcgc tggcacacgg
    12781 catcgcctcc ctgttcggcc gcggcgatgc ggcgcgccgt ccgatcccga tgacgccgga
    12841 agagctgctg gaggcggcga tgctcgtcta catgcaaggc ctcggcttcg gccctgccga
    12901 gtcgggatca gctcagggct gaattggctc cgccgccggc gggcatggta tggccgcccg
    12961 tgccttccta catcctcact ctggtgctgc gatctgcacc ccgagcgtga aatttttctt
    13021 cccgctccct cttgacgccc gggtccagtc attgcaccta tgttaatgta aatgacattt
    13081 acatgaggac gaaagcgatg gcggaggtga tcgaacgcct agacgactgg ggaaagccgg
    13141 cctggatcgc cgtcatggtc cttggcttcg cgattttctg gcccgtcggg ctcgcaattc
    13201 tggcttatct gatctggagc ggacgcatgg gatgtggacg gaacggtgat ctggcgcgct
    13261 ggcaaagccg catggccgag cgttgggagc gcaagaggga gaagtggggc ctgcatggtc
    13321 gcgtctactc ctcgtcaggc aacagcgctt tcgacgagta tcgggaagag acgctgcggc
    13381 ggctcgagga ggagcagcgc gagttccgca gcttcctcga ccgtctgcgc atggccaagg
    13441 acaaggccga gttcgatcag ttcatggccg agcggcgcaa tcggtccagg gatgccggtc
    13501 cgggcgagcc tgggccagag cagcggtgag attacagtgg agtagtgcta agccggacgg
    13561 cgccagatgt ggcgccgtct gcatgtctat ttgccggtta tcgcctcgcg tgcggcgtcg
    13621 catttgctca gcgcggcgct tcagttgcgc tcctcagtgc agggacagct ccactcgatt
    13681 tcggcagcac tgaaaatcag aacgcggcca ccacgccgtc gcctcgtggg tcgacagccc
    13741 cctccagaac tccaattggg gtcctgacga tggctccggc atgcccggtg acttcgtcga
    13801 aggggccaat gggctcgagg tcgtggccgg cacgggcgag agcgtccagg gtttcgccag
    13861 caaatcggct ctctatgcgc aagctcatcc tttgctcgcc ccaggtgcgg ccgagtagcc
    13921 agcgcggtgc ggtcacggcc tgctggagct cctgcccgaa ggcggaatag cgagtgaaga
    13981 tcgccgcctg ggtctgaggc tgaccgtccc cgcccatggt gccgtagacc atgaccctcc
    14041 cgtcgtcgaa cacggcgagc ggaggctgga tcgtgtgaag cggcttgcgc gccggcttaa
    14101 gcgcgttgcg ggccccaggc gcgagctcga agaccgtgcc ccggttctgc cacgtaattc
    14161 ccgtcgcagg gagcaccagc ccggagccga actcccagta aatgctctgg atgcagctga
    14221 ccgcgcggcc ctcgccatcg accaccccca gccacaccgt gtcgccgttc gtgtggccgc
    14281 ctgcccaccg cagcgccgtg caaggatcga tctccgccgc caatgtgtcc agaacgtccg
    14341 gcgtgaggaa atcatcacag tcgacatcca tataggccgg gtccgatatg tgcgcgtcgc
    14401 gcacgcgaaa ggcccgcttc gttgcctcga ccaggccatg cacgaaatcg aacccttccg
    14461 cctcgcggca acctaaccgt tcgaacagac caaggatcat caatgcggcc agcccctgcg
    14521 tgggcggtgg catgttgtag atcgtcgcgc cgaatgcgtg cagcgagagg ggctttacga
    14581 gacgcgctcg ttgagcggcg aggtcctggg cgcgaatggg gctaccagcc gcctcgagat
    14641 cgaaggcgat cgcttccgcc agctcgcctc ggtagaaatc atcgagcccg gccgcggcca
    14701 gccgctccaa agtcgtcgct agggttggtt ggcggaagat ctcgccgacc gcgggcacgg
    14761 ccccgaccga aaggtaatga gttgcgaacc ctggcacagg ctcgagctca tcgcgcttcg
    14821 cggctgtatt ggcatgctgg ctccgggtga cgggataacc ggcccgcgcg tagaaaatgg
    14881 cgtcctcgaa aaggcgcgcc agcggcatcc gcccgcccat ccctcccgcg agctccaccg
    14941 ctgcctgcca gccggagacc gctccggcga cggttatcgc agccagaggc ccgcggttcg
    15001 gaatggaatt catgccgcgg gcccgataga actccgcatc ggccccgctc cacgctgccc
    15061 cacaggcgtc aatcccaacc ggcggtccat cgtctgcgcg gatgagccag aagttgtccc
    15121 cgccgagccc gttcatgtgc gggtagacca cggcgatcgt ggccagcgcg gcgatcatgg
    15181 cctcgatcgc gttgccgccc tctcgcagaa ctctgagacc agcctccgcc gcgagatgat
    15241 gaggcgcaac gaccattccg cgcgtcgccc gcggtgtctg taacatgctc ccttccttct
    15301 caatcaggca cgcatcggct caccaaccga tcgcccgcgg cagccagagc gcgatgtctg
    15361 gatagatgaa gacgaggacc acagcgacca cctgcagcgt cacgaatgga gcaacgccca
    15421 cgtaaatgtg gcgcgtggtg attgacggtg gcgcgacgcc cttgagaaaa aacagcgacc
    15481 agccgaccgg cggggtgagg aaggtggtct gcaacgtcat gccgacaaga attgacatcc
    15541 agaccatgtc gatgccggcg ttgcggaaga tcggcacgaa cagcggcatg acgatgtagg
    15601 agatttcgat ccactcgagg aaaaagccga ggaagaacaa gatcaccagc atgaagatca
    15661 aggcgcccgt cgtgccgccg ggcacggcct cgaacatcgc ctcgaccaga ccatcgccgc
    15721 cgagaccgcg gaatgccagc gagaacgcct gcgatgcgat gaggatgaaa tagatcatcg
    15781 ctgagatgtt gagcgtgttc ctgagcgtgc cgacaagcgt cggccagtcg agccgccggc
    15841 ctagggctgc gatcaggagg gctcctgcgg cccccattga ggctgcttcg gtgggcgctg
    15901 cgatgccacc gatgatcgat ccgagcactg ccagaatgag accgatcggc ggcagcacga
    15961 ccttgaagac gtcgacgacg agttgacggc tggttttcag atcacgctct gcctttggaa
    16021 tgggcggagc cagctcgggt cgaaggagcc cgaggacgaa gacataaacg aggtagatgc
    16081 cggcaagaac caaccccggg atcatggccg cggcaaagat ggtgcctacg gactcggcca
    16141 tgatgtcggc cagcaaaatc agcacgagac ttggcggtat gatctgaccg agcgtccccg
    16201 aggcgcagat gacgccgcaa gcgagcggct tactgtagtt gcgcttgaga aagaccggca
    16261 acgtcagcag gccgagcgtg acgacggtgg cgccgacgat gccagtcgcg gcaccgagca
    16321 gaacaccaac cagaacgatg gccagtccca tcccgcctgg aagcggccca cacaggaagc
    16381 cgataacgtc gagcagatct tcggccagcc gcgattgctc cagcatcacc cccatgaaca
    16441 cgaagagcgg gacggccaga agcgtgtagt tggtggcgac gccataaatc ctgagcggta
    16501 tcagattgaa cagcgtcagc ccgaagccca ggaacccaaa agtaaatccc gagatgagca
    16561 gcgcaatcgc aaccggcacg ccgacgaaca gcaagccgat gaaggacgcg cacattagca
    16621 gcgccagagt ctcaccttcc atgactcacc tgccgagcgc gcgtttggaa taaaggatga
    16681 tgtgcgccac agcctgtagc gccagcagcc cgaacccgag cgggatcatt gatttcagga
    16741 tgaagcggtg aggcagaccg ccgggatcag gagagccctc gtcgatcacg taggcttgat
    16801 tgacgaagtt gagagagagc cagacgacga gaacggaggt gacgaggaga ccgatcgagc
    16861 tgaacaggtt gacaacggcg cgcgtacggg cgccgaagcc g
//
LOCUS       DASVDU010000077         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1593860, whole genome shotgun sequence.
ACCESSION   DASVDU010000077 DASVDU010000000
VERSION     DASVDU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593860"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VF226_05090"
     CDS             complement(<1..743)
                     /locus_tag="VF226_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7073396.1"
                     /translation="MRRVVVTGMGIVSSIGNSTQEVLASLREGKSGIVRAEKYAELGF
                     RCQVHGAPKIAWEEMVPRKPKRFMGPGTAWNYIAMEQAIRDSGLTEQEVQSERTGIIM
                     GAGGPSTSTIVRAAMVAQEKEPKKVGPFEVPKAMCSGPSGTLATAFQIHGVNYSISSA
                     CATSAHCIGNAAELIQWGKQDVMLAGGCEELDWTLSVLFDAMGAMSSGFNDTPERASR
                     AYDKDRDGFVIAGGAGVIVLEELEHAKARG"
     gene            complement(818..1360)
                     /gene="fabA"
                     /locus_tag="VF226_05095"
     CDS             complement(818..1360)
                     /gene="fabA"
                     /locus_tag="VF226_05095"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEX7073397.1"
                     /translation="MADRRSTYEYEDLLACGRGELFGPGNAQLPLPPMLMFDRISTIS
                     EDGGAHGKGQVKAELAVAGNPQCEWFFSCHFKGDPVMPGCLGLDALWQLTGFFLGWLG
                     APGRGRALGVGEVKFTGMVLPSVKRVEYVVDLKRVILRKLKLAIADGSLFADGEQIYS
                     AQDLRVGLFDAGQAPGVSPA"
     gene            1603..2043
                     /locus_tag="VF226_05100"
     CDS             1603..2043
                     /locus_tag="VF226_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX7073398.1"
                     /translation="MQEKATTPTDRDLEQFLRRAGLRPTRQRLVLGSLLFGSEDRHVT
                     AEMLHAEAVAAGERVSLATVYNTLHQFKRAGLLREIAIEGSKAYFDTNTSNHNHFLIE
                     PEGRLIDIPGPCVCIERLPAPPEGMRISHIDVVIRLVPKRPLDR"
     gene            complement(2073..2633)
                     /locus_tag="VF226_05105"
     CDS             complement(2073..2633)
                     /locus_tag="VF226_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7073399.1"
                     /translation="MAGVLRTAMAGLLVLLIATFLPSTAEAQLVAGLPQKKGSSSGLA
                     IPRFVSLKADRVNLRSGPGTEYPTSWVFNRAGLPVEVLNEFEGWRQIRDAEGSTGWVL
                     HSMISGRRTALVLPWELKKGEAPPRVPLRDDDSARARPVAMVEAGVIANISSCDGRWC
                     LVVIGDFRGYIEQDKLWGVYSGEVIR"
     gene            2841..3839
                     /locus_tag="VF226_05110"
     CDS             2841..3839
                     /locus_tag="VF226_05110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEX7073400.1"
                     /translation="MAPTAKKPLVIVTRKLPDVVETRMRELFDARLNVEDKPMSQSEL
                     IEAVKTAQVLVPTVTDRIDRTVLSQAGPQLKLIANFGTGVDNIDYETARNRGIIVTNT
                     PGVLAEDTADMTMALILAVPRRLMEGAAYLKQHNNDWPGWSPTWMLGHRIYGKRLGII
                     GMGRIGQAVARRARAFGLQIHYHNRRPVSEEIEQELEATYWDSLDQMLARMDIISVNC
                     PHTPATYHLLSARRLKLLKPTAYIVNTARGEVIDENELARLIEAGAIAGAGLDVFEHE
                     PAVNPKLLASNRVVALPHMGSATLEGRIDMGEKVIINIKTFFDGHAPPDRVHPAMF"
     gene            complement(3964..4839)
                     /locus_tag="VF226_05115"
     CDS             complement(3964..4839)
                     /locus_tag="VF226_05115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7073401.1"
                     /translation="MKKQDFKTERLGFAPQAGFSRREIMVTTLATGFAAAVQPVMAQT
                     AITTDTAGLDAGEIKIKVQDGEIPAYWAMPAKEGPFPVVLVVQEIFGVHEHIKDICRR
                     LGKEGYMAIAPELYARQGDVSNKSMDEIRSVVAKVPDAQVMSDLDAAVEHARASGKGD
                     TNRLAITGFCWGGRIVWLYAAHNPGLKAAVAWYGRVVGEKSDLTPRHPIDIAAELKAP
                     VLGLYGAADQSIPVSTVDQMRAACAAAGKTCEIIVYPDTPHAFHADYRPSYRAGPAKD
                     GWSRMLAWFRKHGVA"
BASE COUNT          892 a         1702 c         1518 g          866 t
ORIGIN      
        1 ccccgcgcct ttgcgtgctc cagttcctcg agaacgatca ccccggctcc gccagcgatc
       61 acaaagccgt cgcgatcctt gtcataggcc cggctcgcac gctcgggcgt gtcgttgaag
      121 ccggacgaca tggcgcccat ggcgtcgaac agaaccgaca gcgtccagtc gagctcttcg
      181 cagccgccgg cgagcatcac gtcctgtttg ccccattgga tcagctccgc ggcattgccg
      241 atgcagtgag ccgaggtcgc gcaggccgac gagatcgaat aattgacgcc gtggatctgg
      301 aaggccgtgg ccagggtgcc ggacggcccc gagcacatcg ccttcggcac ctcgaacggg
      361 ccaaccttct tgggctcctt ctcctgggcc accatggccg cccggacgat cgtgctcgtc
      421 gaaggtccac cggcgcccat gatgataccg gtccgctcgc tctggacctc ctgctcggtg
      481 aggccggaat cccggatcgc ctgctccatg gcgatgtagt tccaggcggt gcccgggccc
      541 atgaagcgct tcggcttgcg gggaaccatc tcctcccagg cgatcttggg cgccccatgc
      601 acctggcacc tgaagccgag ctcggcgtac ttctccgccc tgacgatgcc cgacttgccc
      661 tcccgcagtg aggcgaggac ttcctgggta ctattcccga tgctggaaac aatgcccatt
      721 ccggtcacga cgacccgtct catatccgac cctttctggt cccttcggtt ggcaggccga
      781 tgcctgatct gcccgattgc gaccgggccc agctctttca ggcgggcgaa acgcccgggg
      841 cctgccctgc gtcgaacagt cctacgcgca ggtcctgggc cgaatagatc tgctctccat
      901 cggcgaagag gctgccgtca gcaatggcga gcttcagctt ccgcaggatc acccgcttga
      961 ggtcgacgac atactcgacc cgcttcacgc tcggcagcac catgccggtg aacttcacct
     1021 cgccgacgcc caaggcgcgg ccgcgaccgg gagcgccgag ccagcccaga aagaagccgg
     1081 tgagctgcca cagcgcatcg aggccgaggc atcccggcat caccgggtca cccttgaaat
     1141 ggcaggagaa aaaccactcg cattgcgggt tgccggcaac ggccagctcg gccttgacct
     1201 gtcccttccc gtgcgcgccg ccgtcctcgc ttatggtgga tatacggtcg aacatcagca
     1261 tcggcggcag cggaagctgg gcatttcccg gcccgaacaa ctcaccgcgg ccgcacgcca
     1321 gcagatcctc gtattcgtat gtcgatcgcc tgtccgccat ggcgcccttt gtgctgcccc
     1381 cttggatgcc ccgcctcgcg caaagcgcag gaatgaaggt ccgcgcgtat ctagcacaat
     1441 cagccaaact tcaaaaaggc aaacggcagg tgcgtcaagg cgctttgccc cctcttacgg
     1501 ggaaagtcca gcctttcggg ccgcacggga gtgccttgtt tcgagctatg gcggtgacta
     1561 tagttggttg caacctagaa aactttcggc cgactgctgg agatgcaaga aaaggcgacg
     1621 accccgaccg accgcgactt ggagcagttt ctgcgccgcg ccggtctcag accgacgcgg
     1681 cagaggctgg tattgggctc gctccttttc ggctcggagg atcgtcatgt gaccgccgag
     1741 atgctgcacg cggaagcggt tgcggccggc gagcgcgtat cactggccac cgtctacaac
     1801 acgctgcatc aattcaagcg cgccggcctt ttacgcgaga tcgccatcga gggatcgaag
     1861 gcctacttcg acaccaatac gtcgaatcac aaccattttc tgatcgagcc cgagggccgt
     1921 ctcatcgata ttcccggccc gtgcgtctgc atcgagcggc tgccggcgcc gccggaagga
     1981 atgcgcatct cccatatcga cgtggtgatc cggctggtgc caaaaaggcc actcgaccgc
     2041 tgagggctgc tcaatcggtg gcccaatcgg cgtcaccgga tcacctcgcc gctgtagacc
     2101 ccccaaagct tgtcctgttc gatgtaaccc cggaagtcgc cgatgacgac gagacaccag
     2161 cggccgtcgc agctgctgat gttcgcaatc acgccggctt ccaccatggc gacgggccga
     2221 gcccgcgcgc tgtcgtcgtc gcgcagcggg acccgcggcg gcgcctcgcc cttcttgagc
     2281 tcccagggaa gcacgagcgc agtgcgtctg cccgaaatca tcgagtgcag aacccagcct
     2341 gtcgaaccct cggcgtcacg gatctgccgc cagccctcga actcgttcag cacctcgacg
     2401 ggcagacccg ccctgttgaa cacccacgag gtggggtatt ccgtccccgg cccactccga
     2461 aggttcaccc gatcagcctt caggctgacg aatcgtggaa tggcaaggcc actggagctg
     2521 cccttcttct gcggcagccc ggcgacgagc tgggcctcgg cggtagaagg cagaaaggtt
     2581 gcgatcagca ggacgaggag acctgccatt gcggttctca acacccccgc cattcactcc
     2641 ctccccgcta agctccgtgc agaaatcccg cgcgccggtc cgcgcacccg tggttgacat
     2701 tctgtcccga cggcccgagc gatctgctcc aactctttgt cattgcgttg gccttctgtt
     2761 agacaaatcc tgaacctggt actccaccaa agacctccat ccggcacggc ttcgagcgca
     2821 agagaacgac agaatcgccc atggcaccca cggccaagaa acctctcgtc atcgtcacgc
     2881 gcaagctccc cgacgtcgtc gagactcgca tgcgagagct cttcgatgcg cgactcaacg
     2941 tggaggacaa gccgatgagc caaagcgagc tcatcgaggc cgtcaagacc gcgcaggtgc
     3001 tggttccgac cgtcacggac cggatcgatc gcacggtcct gtcgcaggcc gggccgcagt
     3061 tgaagctgat cgcgaacttc ggcaccgggg tcgacaacat cgattacgag accgcccgca
     3121 accgtggcat catcgtcacg aacacccccg gcgtgctggc cgaggacacc gccgacatga
     3181 cgatggcgct gatcctcgcc gttccgcgcc gattgatgga gggggcggcg tacctgaagc
     3241 aacacaacaa cgactggccc ggctggtcgc cgacgtggat gctgggccat cgcatctacg
     3301 gcaagcggct cggcatcatc ggcatggggc gcatcgggca ggccgtggcc cgccgcgctc
     3361 gagcgttcgg gttgcagatt cactatcaca accggcgtcc ggtctcggag gagatcgagc
     3421 aggagctgga ggcgacctac tgggatagcc tcgatcagat gctggcgcgt atggacatca
     3481 tttccgtgaa ctgcccgcac acgccggcga cctatcacct gctctcagct cgccgcctga
     3541 agctgctgaa gccgaccgca tacatcgtca acaccgcgcg tggcgaggtg atcgacgaga
     3601 acgagctggc gcggctgatc gaggcgggcg cgattgcagg cgcggggctc gacgtcttcg
     3661 agcacgagcc ggccgtcaac ccgaagctgc tcgcatcaaa tcgcgtcgtt gcgctgccgc
     3721 acatggggtc cgcaacgctc gaggggcgca ttgacatggg tgagaaggtc atcatcaaca
     3781 tcaagacctt cttcgacggc cacgctccac ccgatcgcgt gcacccggcg atgttttgag
     3841 cgctcgcacc cgcgaagccg cacggctgtg cgggcgggat cgcgcgcaca gtgtccgccg
     3901 ttcgagccca ggttagccgc aagagccagc ggagtaagcg ttggctcccg ctagcttcac
     3961 ttctcacgcc actccatgct tgcggaacca ggccagcatg cgtgaccagc cgtccttggc
     4021 aggccccgca cggtagctgg gccggtagtc ggcatggaag gcgtgcggag tgtcgggata
     4081 gacgatgatc tcgcacgtct tgccggcagc agcgcaggcc gcgcgcatct ggtcgaccgt
     4141 gctgaccggg atgctctggt cggccgcccc gtagaggccg agcaccggcg ccttcagctc
     4201 ggccgcaata tcgatcgggt ggcgcggcgt cagatcgctc ttctctccga caacgcgacc
     4261 ataccaggcg accgcagcct tgaggcctgg attgtgagcg gcgtagagcc agacgatccg
     4321 cccgccccag cagaagccgg tgatggccag ccggttcgtg tcgcccttgc ccgacgcgcg
     4381 cgcatgctcg acggcggcat cgaggtcgct catcacctgg gcgtcgggca ccttggcgac
     4441 gacggaccgg atctcgtcca tcgacttgtt ggaaacatcg ccctgccggg catagagctc
     4501 cggcgcgatc gccatgtagc cttccttgcc caaccggcgg cagatgtcct tgatgtgctc
     4561 gtgaacgccg aagatctcct gcacgacgag caccacgggg aacggtccct ctttcgcggg
     4621 catcgcccag taggcgggga tctcgccgtc ctgcaccttg atcttgatct cgcccgcatc
     4681 gaggccggcg gtatcggtcg tgatggccgt ttgcgccatc acgggctgca ccgcagccgc
     4741 aaagcctgtg gccagtgtcg tcaccatgat ttcccggcgt gagaagccgg cctgtggcgc
     4801 gaagcccaga cgctccgttt tgaagtcctg cttcttcatg ggtcctccgc tgttgacgtc
     4861 gcgagtggcg gtaccggcga gactacaggc agacagccga atgtcatcca acgctcgacc
     4921 gggtcacgga attgtgcttt cccagtgtcg ccggcagggc gggccgacgc cgcccgat
//
LOCUS       DASVDU010000078         8917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_116945, whole genome shotgun sequence.
ACCESSION   DASVDU010000078 DASVDU010000000
VERSION     DASVDU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116945"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VF226_05120"
     CDS             <1..1193
                     /locus_tag="VF226_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7073402.1"
                     /translation="ETLAHVISVGKSATGSAQVFWDLLEQASDAERATDTGPDDPAIL
                     MYTSGTTGNPKGVLHAGRVLLGHLPGVMLPQDMFPKPGDRFWTPADWAWAGGLLDVLL
                     PSLYHGVAVVGSARAKFDPEWAFSFMARHGVRNVFMPPTALRLMRQVPDPRSRFGYDV
                     RSIGTGGEKLGEDMIAWGQETFGLTLNEFFGQTEVNLVVGNSGRLFPVRAGSMGRAIP
                     GHIVEIVDAQGNVVPPRQTGIVAVKRPDPVMFLEYWRRPDATRDKFRGDWCLLGDVAS
                     KDEEGYFWFEGRDDDIINSAGYRIGPGEVEECLQKHPAVALAGVVGAPDPVRGEIVVA
                     FVVPAPGVQPSDELAAEIQSFVRDRLAAHEYPRRIRFIAEMPMTVTGKIRRVDLRAMD
                     KAGA"
     gene            1403..2833
                     /locus_tag="VF226_05125"
     CDS             1403..2833
                     /locus_tag="VF226_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEX7073403.1"
                     /translation="MANVSFLQELLTTVAEQGRQLLQWSGRSWDASEDIAELAEALVS
                     VRGEASGVAIADQILRLYQGLSSEQKRTFFQFLAHSLQPREEKVREAASAYLEQPSQE
                     ALSRLQAAVESPRQEFFRRLNLAPGATGKIVAMRSDLLKLLASEPSLSVVDADLRHLL
                     YSWFNRGFLVLRRIDWQTPAAILEKIIAYEAVHAIKGWDDLRRRLDPKDRRCFAFFHP
                     SLVDEPLIFVEVALADDMADSIASLIEDASDPDGKTEPTTAIFYSISNCQDGLRGISF
                     GNFLIKQVVEDLAREMPTLKTFVTLSPVPQFARWLASVVNEDETGLITPELRQVLQNL
                     SDTHWIWDPERVQTLEGPLTQLAAHYLVNAKSSDGRPVDPVARFHLGNGARLERINWL
                     ADTSAKGLRESHGIMVNYRYDLKEFERNHEAYANEGVVATSRSVRSLLRTAPKLRVPK
                     NEPRMLPAPESGDEAQRTAEMEPVER"
     gene            complement(2910..4064)
                     /locus_tag="VF226_05130"
     CDS             complement(2910..4064)
                     /locus_tag="VF226_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073404.1"
                     /translation="MRAQTSPDHSPEPAAAKASSGLYVATWAVLAAAASGYMGILLTQ
                     PDWAAPLTTQSLRTEESKPAAPEVVAQLTGEIHTLRTTVADLQRELIEVKSTAAAKED
                     IEAIREDVRAAIAPPVVESSAQEPPGLEQPTPMAAAADHSVPVVINPQSTEPQLRATQ
                     TEPPEQNIVRVNPQEEAFPEENQVVLDHLGVKIVNATPIQHTQAAPDTDPAVPEDKLA
                     RAERQDAPRSVAVAPPPPPLPLKKVALLDASSANSVTSRPVETIATSPPRSALPLATG
                     SLPPSAPSQITFGPATVTPASEAYAIHLDAGPSLEALRLRWSVLHERHRSALGDLEPR
                     YIAGGSPTAPSYELLAGPIASLEEASRICALLRAKAVACSVGVPFEGEAL"
     gene            4376..5737
                     /gene="glmU"
                     /locus_tag="VF226_05135"
     CDS             4376..5737
                     /gene="glmU"
                     /locus_tag="VF226_05135"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX7073405.1"
                     /translation="MTSRPLLTIVLAAGKGTRMKSSIPKVLHRVGGLSLLGHVLRSVR
                     EAGSDRIAVVIGPEMDAVHAEVEARAPGAEVFIQDRQLGTAHAVLAARAAIMGHTGDV
                     LVVFGDTPLLTAGTLISLSSCLDAETAVAVLGFEAADPTGYGRVLCDSTGAVAAIREH
                     KDASATERAVTLCNGGVMAFRGEQMLPLLDAIGTDNAQGEYYLTDAVAIARAKGYRVA
                     VRTCPEEEVLGVNSRNQLAMAEAVFQRRFRERVMAEGATLIAPETVWFSHDTQVGRDV
                     MIEPNVFFGPRVVVEDEVLIHANCHMEGARLRRGAEIGPFARLRPGADLGPRSKIGNF
                     VEVKNTTVEEGAKANHLSYLGDGRVGAGANIGAGTIFCNYDGFFKYRTEIGPGAFIGS
                     NTSLVAPVSVGEGAFIGSGSVVTKDVPADALALERSEQITREGWAAKFRTIMKRRKAQ
                     TRS"
     gene            5833..7653
                     /gene="glmS"
                     /locus_tag="VF226_05140"
     CDS             5833..7653
                     /gene="glmS"
                     /locus_tag="VF226_05140"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7073406.1"
                     /translation="MCGIVGILGTKPVAGDLVDALRRLEYRGYDSAGIATVENGHLDR
                     RRAEGKLRNLEARLLKEPLKGRAGIGHTRWATHGRPIERNAHPHMNDRVAVVHNGIIE
                     NFQELKDELVAKGHAFETETDTETVLHLVTDYLDQGHSPVEAARLALRRLTGAFALGI
                     VFAGHDDLMIGARQGPPLAVGHGKGEMYLGSDAIALAPFTDAITYLEDGDWVVLTREG
                     AKFYDIAGNPVERPQVKSVASSLLVDKGNYRHFMAKEIHEQPEVISHTLANYLDMGTG
                     KVVFPDLGVDLAKVARVTITACGTAYYAGLVGKYWLERYARVPVEIDVASEFRYRESP
                     LPENGLAIFVSQSGETADTLASLRYCKAQGQKIASVVNVRTSTIARESHAVLPTLAGP
                     EIGVASTKAFTCQLSVLACLAIAAGRARGHISEAQEKELVRALTEVPRHISTILRSEA
                     AYETLAGALHKAKDVLYLGRGQNYPLALEGALKLKEISYIHAEGYAAGELKHGPIALI
                     DENVPVVVVAPEDDLFEKTVSNMQEVAARGGQIILVSDAPASKAGCNLHTHLEMPRAH
                     PFTNPLLYAVPAQLIAYHTAVVMGTDVDQPRNLAKSVTVE"
     gene            7766..8797
                     /locus_tag="VF226_05145"
     CDS             7766..8797
                     /locus_tag="VF226_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7073407.1"
                     /translation="MTRARTTGARQTNGVVGAVAAALTAGLILFGIPAAVAAKGKTAP
                     AGEVDQIIETAVSALEAGKPDLAVQELTSAISGGKITQKKMARALYFRGLAYRKQNKP
                     AQAIADFTSALWIKNGLDAEQRADALLNRAAAYRDAGLTDQAEADEQRVAASRQSRRA
                     AAASPAETPSAPASSSGGGLGSFFNALFGGGSASEQTTSAGFATSVSQPASHGGKSGR
                     APEVSAWSNKGGTATAGWAESTVVRKASASRSTPTTTASTGRASRKGQFIQVAAVRSR
                     KEAQSVAAQLARIGAVAGHHASIDEAVIGNMGTLYRVRLGPFASAKEVQSACPSLRAA
                     GFDCMIVDQ"
BASE COUNT         1578 a         2825 c         2984 g         1530 t
ORIGIN      
        1 tcgagacgct ggcgcatgtg atctcggtcg ggaaatcagc aacgggctcg gcacaggtct
       61 tctgggatct gctggagcag gccagcgatg ccgagcgcgc gaccgatacg ggtccggatg
      121 atccggcgat cctgatgtac acgtccggca cgaccggcaa tccgaaggga gtcctccacg
      181 cgggccgtgt tctcctcggc cacctgcccg gtgtgatgct gccgcaggac atgttcccga
      241 aaccgggcga tcgtttctgg acgcccgccg attgggcctg ggcgggcggc ttgctggacg
      301 tcctcctgcc gtcgctctat cacggcgttg ccgtagttgg ctcggcgcgg gcgaagttcg
      361 accccgagtg ggccttctcg ttcatggcgc gccacggcgt acgcaatgtc ttcatgccgc
      421 cgacggcgtt gcggctgatg cggcaggtcc cggatccacg ctcgcgcttc ggctacgacg
      481 tgcgctcgat cggcaccggc ggcgagaaac tcggggaaga catgatcgcg tggggccagg
      541 agacgttcgg cctgacgctc aacgaattct tcggccagac cgaggtcaac ctcgtcgtcg
      601 gcaactccgg ccgccttttc cccgtgcgcg ccggctcgat gggccgcgcg ataccgggcc
      661 atatcgtcga gatcgtcgac gcgcagggca acgtggtgcc gccgcgtcag accggcatcg
      721 tcgccgtgaa gaggcctgat ccggtgatgt tcctcgaata ctggcgccgt ccggacgcga
      781 cccgcgacaa gttccgcggc gactggtgcc tgctgggcga tgtcgcgagc aaggacgagg
      841 agggctactt ctggttcgag ggccgcgatg acgacatcat caactcggcc ggctaccgca
      901 tcggccccgg tgaggtggag gaatgcctgc agaagcaccc ggctgtcgcc cttgcagggg
      961 tggtcggcgc gcctgatcct gtacgcggcg agatcgtcgt cgctttcgtc gtgcccgcgc
     1021 cgggcgttca gccctccgat gagctggccg ccgagatcca gtccttcgtc cgcgatcgcc
     1081 tcgcggctca cgagtatccg cgccgcatcc gcttcatcgc cgagatgccg atgacggtga
     1141 ctggtaagat tcgccgcgtg gatctccggg ccatggacaa ggccggggca taacagtcct
     1201 tataaccggt acgcaccagg atggtgcgca agcgattgcg caagtgtgcc gcccttgaga
     1261 tcaagaattt tttaggcccc ggcatctgtc atggcgtttc cttcgaaccg ttgttatttg
     1321 ctgatgagac agccattgcc tggatgggca cgtcgttagc aagctaccag gcaggaccca
     1381 tagcagcgcg aaaggatcgg tcgtggcgaa tgtttcgttt ctgcaggagc tgctcactac
     1441 ggttgccgag caggggcggc agctgctgca gtggtcgggg agaagctggg acgccagcga
     1501 ggacatcgcc gagctcgcag aggcgcttgt atcggtacgc ggcgaggcgt ccggtgttgc
     1561 catcgctgat cagatcctga ggctctacca aggtctgtcc agcgagcaga agcgtacgtt
     1621 cttccagttc ctcgcgcact cgctgcagcc tcgggaggaa aaagtccggg aggcggcttc
     1681 ggcctacctg gagcaacctt cgcaggaggc gctgtcgcgg ttgcaagccg cagtggaaag
     1741 cccgcggcag gagttcttcc gcaggctcaa tctcgcaccg ggagccaccg gcaagatcgt
     1801 cgcgatgcgg agcgatctgc tgaagctgct ggcgagtgag ccttcgctct cggtcgtcga
     1861 cgcggatctg cggcatctgc tctattcctg gttcaacagg ggcttcctgg tgctgcgccg
     1921 gatcgactgg cagacgccgg cggcgatcct ggagaagatc atcgcctacg aggccgtgca
     1981 cgcgatcaag ggctgggacg acctgcgtcg caggctcgat ccgaaagacc ggcgctgctt
     2041 cgccttcttc catccatcgc tcgtcgacga accactgatt ttcgtcgagg tcgccctggc
     2101 cgacgatatg gcggacagca tcgccagcct tatcgaagat gcgtcggacc cggacggcaa
     2161 gaccgagccg acgacggcga tcttctattc gatctccaac tgccaggacg gcctgagagg
     2221 aatttcgttc ggcaatttcc tcatcaagca ggtggtcgag gatctcgcgc gcgaaatgcc
     2281 gacgctcaaa acgttcgtca cgctatcccc ggttccgcag ttcgcgcgct ggcttgcctc
     2341 cgtggtgaac gaggatgaga ccggcctcat cacgccggag ctgcggcagg tactgcagaa
     2401 cctgtccgat acgcattgga tctgggatcc ggagcgggtg cagacattgg aggggccgct
     2461 gacccagctc gcggcacact acctggtcaa tgcaaagtcg agtgacgggc ggccggtcga
     2521 tccggtggcg cgattccatc tcggcaacgg cgcgcgtctg gaacgcatca actggctggc
     2581 agatacgtca gccaagggat tgcgcgagtc ccacgggatc atggtgaact accgctatga
     2641 cctcaaggag ttcgagcgca atcacgaggc ctacgcgaac gagggcgtcg tcgccacctc
     2701 gcgctccgtg cggagcctgc tgcgcacagc gccgaagctg cgtgtgccga aaaacgagcc
     2761 gcgcatgctg ccggcaccgg aaagcggcga tgaggcgcaa aggacagcgg agatggagcc
     2821 ggtggagcgc tgaatgattt gccggcggtc gtgaggtcgg cagatcggca atcggggtga
     2881 agcaagcgcc gactgccgac tgccgatctt cagagtgcct cgccctcgaa gggcacgccg
     2941 accgagcagg caacggcctt ggcacggagc agggcgcaga tgcggctcgc ttcttcgagc
     3001 gaggcgatgg gaccggccag aagctcgtag cttggcgcag tcggtgagcc acccgcgata
     3061 tagcgtggct caaggtcccc gagcgcgctc cgatgacgct catgcagcac gctccagcgc
     3121 agccggagag cttccagcga cggcccggca tcgagatgga tcgcgtaggc ctccgaggcc
     3181 ggggtcacgg tcgccggacc aaaggtgatc tgcgacggag cggaaggcgg caggctgccg
     3241 gtcgccagag gcagtgcgga tctgggtggg ctggtcgcga tggtctcgac cggacgggat
     3301 gtgacactgt tggcggagct cgcgtccagc aacgccacct ttttcagagg tagcggcggc
     3361 ggtggcggcg cgaccgcgac gctcctcggg gcatcctgcc gctcagcccg cgccagcttg
     3421 tcctccggca cagccgggtc ggtatcaggg gcagcttggg tgtgctgtat cggagtcgcg
     3481 ttcacgatct tgaccccgag gtggtcgaga acgacctggt tttcctcagg aaaagcctct
     3541 tcctgaggat tgacgcgaac gatgttctgc tcgggcggct ctgtctgggt agctctgagc
     3601 tgcggctcgg tagattgggg gttgatgaca acgggcacgc tgtggtccgc agcggcggcc
     3661 attggagtgg gctgctccaa accgggcggc tcctgagctg aagactccac gacgggcgga
     3721 gcgatggctg cgcgcacgtc ctctcggatc gcctcgatat cctccttggc ggcagccgtc
     3781 gacttcacct cgatcagctc gcgctgcagg tctgcgaccg tcgtgcgcaa cgtatggatc
     3841 tcacccgtca gttgcgcgac gacctcgggg gctgccggtt tggactcctc cgtgcggagc
     3901 gactgggtcg tgagcggggc ggcccagtcg ggctgcgtga ggagtatgcc catgtagccc
     3961 gacgccgcgg ccgcaagcac cgcccacgtg gccacgtaaa gtcccgagga ggcctttgcc
     4021 gccgccggct cgggcgagtg atccggtgaa gtttgcgctc gcatggttgg ctcgaacctc
     4081 gcctcgctca ggccggcgca tccggagcac ggatcgcgcg gccaagccaa tgattcgctt
     4141 gcgccaaacc gtcgtccgag tccgttcatt gggtcaacgg atcaggagga gccgcgggcc
     4201 gcagccctgt ggattgttca aaagcgttgc aactcagggg cgcccccggc agatgacatc
     4261 ggcgtgccgt ggtgcgcttg caaagcgttt ccgcatgaga caccgttcgc cgcgggggca
     4321 gccgcctcga acgaaccgcc gcgcgtcacc agacaggctt tcagccgggg aacacatgac
     4381 ctctcgtcca ctgctcacca tcgtcctcgc ggccggcaag ggcacgcgga tgaaatcgtc
     4441 catacctaag gtgctgcacc gggttggcgg gctttcgctg ctggggcacg tgctccgtag
     4501 cgtgcgagag gcaggaagcg accgtattgc cgttgtgatc ggtcccgaga tggatgccgt
     4561 acacgcggag gtcgaggcac gcgcgccggg cgctgaggtc ttcatccagg atcggcagct
     4621 tgggacggcg cacgcagtcc ttgcggcacg cgcagccatc atggggcaca caggcgatgt
     4681 gctggtcgtc ttcggcgata cgccgctcct gacggcaggg acgctgatta gcctgtcaag
     4741 ttgcctcgac gccgaaaccg ccgtcgccgt cctgggattc gaggccgccg atcccacggg
     4801 ctacggccgg gtgctgtgcg attccacagg cgccgtcgcc gccatccgcg agcacaagga
     4861 cgcaagcgcc accgagcgtg cggttactct gtgtaacgga ggcgtcatgg cgttccgagg
     4921 ggagcagatg ctcccgttgc tcgacgccat cggcaccgat aacgcgcagg gcgaatacta
     4981 cctgacggac gcagtcgcga tcgccagggc caagggttat cgtgtcgctg tccgcacctg
     5041 cccggaagag gaggtgttgg gcgtcaattc ccgcaaccag ctcgcgatgg cggaggccgt
     5101 tttccagcgt cgcttccgcg agcgcgtgat ggctgaaggc gcgacgctca tcgcacccga
     5161 gacggtctgg ttcagccatg acacgcaagt cggccgcgac gtgatgatcg agcccaacgt
     5221 attcttcggt ccgcgcgtcg tcgtcgagga cgaggtcctg atccacgcca attgccacat
     5281 ggaaggcgca cgcctgcgcc gtggcgcaga gatcggcccg ttcgcgcggt tgcggccagg
     5341 cgccgacctc ggtcctcgct cgaagatcgg caacttcgtc gaagtcaaga acacgaccgt
     5401 cgaggaaggc gccaaggcga accacctgtc ctacctggga gacggtcgcg tcggcgccgg
     5461 tgcgaacatc ggtgcaggga cgatcttttg caactacgac gggttcttca aataccggac
     5521 ggagatcgga ccgggcgcct tcattggctc gaacacgtcc ctggtggcgc ccgtttccgt
     5581 tggggagggc gcattcatcg gctcgggaag cgtggtcacg aaggatgtcc cggcggatgc
     5641 gctggcgctc gaacggagcg agcagatcac acgcgaaggt tgggcggcca aattccgcac
     5701 aatcatgaaa cgccggaaag cgcagacgcg ttcgtaggat tcgaaaaacc aagcaatacg
     5761 gtttgagttg agtccaaggc agccacgacg tagcaggagg gcaacagacc ttctgaaaaa
     5821 ggatgcttcg caatgtgcgg gatcgtcggc attcttggca cgaaacccgt tgcaggggac
     5881 ctcgtcgatg cgctccggcg gctcgagtat cgcggctatg attcggcggg catcgccacg
     5941 gtggagaacg gccatctcga ccggcgccgc gccgagggca agctcaggaa tctcgaagcg
     6001 cggcttctga aggagccgct caagggccgc gccggcatcg gccatacccg ctgggcgacg
     6061 cacgggcgcc cgatcgagcg caatgcccat ccgcacatga acgaccgcgt cgctgtggtc
     6121 cacaacggca tcatcgagaa cttccaggag ctgaaggacg agctcgtcgc caaggggcac
     6181 gcgttcgaaa ccgagacgga caccgaaacc gtcctccacc tcgtcaccga ctatctcgat
     6241 cagggccact cgcccgtcga ggccgcccgc ctggccttgc ggcgcctgac gggcgccttc
     6301 gcgctgggca tcgttttcgc cggccacgac gatctcatga tcggcgcccg gcaggggccg
     6361 ccgctggccg tcggccacgg caagggcgag atgtacctcg gctccgatgc gatcgcgctg
     6421 gccccgttca ccgatgccat cacctacctt gaggatggcg actgggtggt gctgacccgc
     6481 gagggcgcga aattttacga tatcgccgga aatccggtcg agcgtccgca ggtgaagtcg
     6541 gtggcgagct ccctgctggt ggacaagggc aactaccgcc acttcatggc caaggagatc
     6601 cacgagcagc cggaggtcat cagccacacg ctggccaatt atctggatat gggaacgggc
     6661 aaggtggtgt tcccagacct cggcgtggat ctggcgaaag tcgcccgcgt gaccatcacc
     6721 gcctgcggca cggcctatta cgcagggctc gtcggtaagt actggctcga gcgctacgcg
     6781 cgtgttccgg tcgagatcga tgtcgcctcc gagttccgct accgcgagag cccgttgccg
     6841 gagaatggcc ttgccatctt cgtctcgcag tcgggcgaga cggccgacac gcttgcctcg
     6901 ctgcgctact gcaaggcgca agggcagaag atcgcctccg tcgtcaacgt acggacctcg
     6961 acgatcgcgc gcgaatctca cgccgtcctg ccgacgcttg ccggtccgga gatcggagtt
     7021 gcctccacga aggcgttcac gtgccagctc tcggttctcg cttgtctcgc gatcgccgcg
     7081 gggcgcgcgc gcggacacat cagtgaagcc caggaaaagg agctcgtccg ggctctgacc
     7141 gaggttccac ggcatatctc gacgattctg cgcagcgaag cagcctacga gacactcgcc
     7201 ggcgcactgc acaaggcaaa ggacgtcctc tacctgggtc gtggccagaa ctatccgctc
     7261 gcgctcgaag gggcgctgaa gctcaaggag atctcctaca tccacgccga aggctacgct
     7321 gccggtgagc tgaagcacgg acccatcgcg ctcatcgacg agaacgtgcc ggtggtggtc
     7381 gtcgctcccg aggatgacct tttcgaaaag accgtttcca acatgcagga ggtggcggcg
     7441 cggggtggcc agatcattct cgtctcggac gcacccgctt caaaagcggg ctgcaatctg
     7501 cacacgcacc tggagatgcc gcgggcgcac ccgttcacga atccgctgct ttatgccgta
     7561 cccgcacaac tgatcgccta tcacacggcc gtcgtgatgg gcacggacgt ggatcagccg
     7621 cgtaatctgg ccaaatcggt aactgtggag taaggctggg gactgacgag attcggcctg
     7681 tcgccgaatt gcctcaaacc gatgcttggc ataggttccc tctgcgagtg gaggtgctgc
     7741 gcgtacttca gacgacgggg acgcgatgac gcgagcgcgc acgaccggag cacgacagac
     7801 gaatggcgtc gtcggggccg ttgcagcggc gctgacggca ggtctcatcc tgttcggcat
     7861 ccctgccgca gtagcggcga agggcaagac ggctccagca ggcgaagtcg atcagatcat
     7921 cgagactgcc gtttcagccc tggaggcagg gaaaccggat ctggcggttc aggagctcac
     7981 ctcggccatt tcaggcggca agatcaccca gaagaagatg gcgcgtgcgc tctatttccg
     8041 cgggctcgcc tatcgcaagc agaacaagcc ggcccaagcc atcgccgatt tcaccagcgc
     8101 cctttggatc aagaacgggc tcgacgcaga gcagcgggcg gacgccctgc tgaaccgtgc
     8161 cgcggcgtac agggatgccg gcctcaccga ccaggccgag gccgacgagc aacgcgtggc
     8221 tgccagccgg caaagccgcc gggctgcagc agctagcccc gctgagacgc catcggcgcc
     8281 ggccagcagc tctggcggcg gcctcggcag cttcttcaac gctctcttcg gtggcggttc
     8341 ggcgtccgag cagacgacgt cggcggggtt tgccacctcc gtttcccaac cggcttcgca
     8401 tggcggcaaa tccggtcgcg cacccgaggt ttctgcatgg tcgaacaagg gcggcaccgc
     8461 gacggccggg tgggccgagt cgacggtggt gcgcaaggcg agtgccagcc ggtccacacc
     8521 gaccaccacc gcgtctacgg ggcgcgcatc ccgcaagggg cagttcatcc aggtggcggc
     8581 agtccggagc cgcaaggaag cgcaatctgt cgcagcgcag ctcgcgagga tcggcgccgt
     8641 tgccggccac catgcgtcca tcgacgaggc tgtcatcggc aacatgggca cgctctatcg
     8701 cgtgcgtctc ggtccgtttg ccagcgcaaa agaagtgcaa agcgcctgcc caagcctgcg
     8761 tgctgccggg ttcgactgca tgatcgtcga tcagtagagc ccatcggcgc ttccggctcg
     8821 cggtctggta tcctaccgga cttaacgcct attgcgcgac ggctctaatc cggcttccct
     8881 gtcgggggat gcaagtatcg ggaggccctg cgccgcg
//
LOCUS       DASVDU010000079         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_446461, whole genome shotgun sequence.
ACCESSION   DASVDU010000079 DASVDU010000000
VERSION     DASVDU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446461"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF226_05150"
     CDS             <1..195
                     /locus_tag="VF226_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073408.1"
                     /translation="LPDNLCYMLVNGEPREAIAVRFDYALRADGQIEQNQYDIDLRSA
                     DLVEEDFVWVKGLYDDFLST"
     gene            220..1263
                     /gene="selD"
                     /locus_tag="VF226_05155"
     CDS             220..1263
                     /gene="selD"
                     /locus_tag="VF226_05155"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX7073409.1"
                     /translation="MTTIRLTALAHGGGCGCKLAPSVLQQLLADQPAAGAYKQLLVGT
                     ESADDAAVWQLDENTCIIATTDFFMPMVDDPFDFGKIAATNAISDVYAMGGTPIMALA
                     ILGMPVDKIPTGAVREILKGGASVCAAAGIPVAGGHSIDCPEPVYGLAVIGTCRPEHL
                     RRNTGARAGDALILTKPIGVGIYSAAIKKGELDAAGYSEMISSTTLLNRIGPELSLDP
                     DVHAITDVTGFGLLGHALEMARGSGVTVEIEADSVPLFTQAARLAQQGFVTGASRRNW
                     ASYSEHVTLPANLPEWRRHLLTDPQTSGGLLVACTAEKAPAIVKRIEEAGYSSARILG
                     RVEDGNDVVKVAA"
     gene            complement(1373..2545)
                     /locus_tag="VF226_05160"
     CDS             complement(1373..2545)
                     /locus_tag="VF226_05160"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX7073410.1"
                     /translation="MTADGTGFEAVGLLDPASEEAQRRPRKRWRLWDLHRGIHGALLA
                     LSFTDDEFRRLLRRARVGIPAEARYYDIHRHLCDACTTRNALSELIEKALDQKFATKI
                     VALRGTRTGEALEAKWQEAWSKGRGLAALFWPFLSHPCATDEIRAGFYADVHALFYRA
                     LSERERLLEKVENLEHRCRALEKRHREELSALEQRLERALRPKVIDATSSSDSDAIAT
                     LQIRFASATQRAARAEARVRTLEERLRHRRNQARKVKWSVAPEPNGAGTLSKDYEAAS
                     GSDDGDAETDLQIPGVRLAYVGGRPGSRERIREFCEKRGATLIDHDGGLEESPVRLDQ
                     LLERADVVFYPVDCISHEAAQHLKSHCKRAEKPFIPLRNSSISEFRRALKSWCGVS"
     gene            2729..>3552
                     /locus_tag="VF226_05165"
     CDS             2729..>3552
                     /locus_tag="VF226_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7073411.1"
                     /translation="MDTHDFPDLMTVSEVADYLRVRERTIYELVRTQRIPSCKLSGKL
                     LFPKRLIELWVAQSADYPQATVHLSAPPPVIAGSHDLLLEWSVRESNCDLAILVGGST
                     AGLHRLLAGQAVACGLHLIDPESGEYDASALTRTLPGLDFVVIEWARRLQGLVVASGN
                     PLKIGSIADLRKRRVRIATRQPGSGTAILFSKLLADAGLTIEELDIIGRPMSSHTDVA
                     DAIRHGKADAGLAIASVTREEGLDFVPLHWERYDLVVRRVEYFEPPLQRLFAFTRT"
BASE COUNT          667 a         1107 c         1057 g          721 t
ORIGIN      
        1 ctgccggata atctttgcta tatgctggtg aatggggagc cgcgggaagc catcgccgtc
       61 cggttcgatt atgctcttcg agcagacggc caaatcgagc agaaccagta tgacatagac
      121 cttcgctccg ccgatctcgt agaggaagat ttcgtttggg tgaaaggact ttacgatgat
      181 ttcctgtcca cttagcgccc gccattgcta ggaggaatga tgacgacaat tcgcttgacg
      241 gctctcgccc acggcggcgg ctgtgggtgc aagcttgcgc catccgtcct gcagcagctc
      301 ctggctgacc agccggcagc tggtgcctac aagcagctac tcgtcggcac cgagtcggcc
      361 gatgatgctg ccgtctggca gctcgatgag aacacctgca tcatcgcaac tacggatttt
      421 ttcatgccga tggtcgatga tcctttcgac ttcggcaaaa tcgcagcgac gaacgccatt
      481 tcagacgtgt atgccatggg tgggacgccg ataatggcat tggccatcct cggcatgccc
      541 gttgacaaaa tcccaactgg ggcggtgcgc gagatcctca aaggtggtgc atcggtctgt
      601 gccgccgccg gcattccggt tgccggcggt cattcaatcg actgtcctga gccggtttac
      661 ggcctggccg tgattggcac atgccggccg gaacacctgc gtcgcaatac gggagcgcgc
      721 gccggggatg cgctgatcct caccaaaccg atcggtgtcg ggatttattc ggccgcgatc
      781 aagaagggcg agcttgatgc tgcgggatac agtgagatga tcagcagcac gaccttgctc
      841 aatcgcatag gccctgagtt gtcgttggac cccgacgtgc acgccattac agacgtgacc
      901 ggctttggcc tgcttggcca cgcactcgag atggctcgcg gctcaggtgt cacggtcgaa
      961 atagaggcag acagtgtgcc gttattcacg caggcagccc ggcttgcgca gcagggattc
     1021 gtgactggcg cttcacgtcg gaattgggcg agctatagcg aacacgtgac gcttccagcg
     1081 aacctgccag aatggcggcg gcacttgctt accgatccgc agacgtccgg aggtcttctc
     1141 gttgcgtgca cggcggagaa ggcccctgcc atcgtaaagc gcatagagga ggccgggtat
     1201 agctctgcga gaatcctcgg ccgtgtagag gacggcaatg atgtggtgaa agtcgccgcc
     1261 taaggagcca gcgagatcat cccgcaggcc gccgctggat gatggctgcc gcgtctgatg
     1321 cgttagccac tacgccgcgt tggcattcgc cgtttagcgc agcgcagccc gttcaactca
     1381 cgccacacca gcttttgagg gcacgccgaa attccgaaat gctcgaattg cgcagcggaa
     1441 tgaatggttt ctcggcacgc ttgcagtgtg acttcagatg ctgcgcggct tcatggctga
     1501 tgcaatcgac cgggtaaaag acgacgtccg cccgttcaag gagttgatcg agccgcaccg
     1561 gcgactcctc caagccaccg tcgtggtcga tcaaggttgc gccccgcttt tcacagaact
     1621 cgcggatccg ctcccgcgag cctgggcggc cgccaacgta agcgaggcgc acaccgggga
     1681 tctgcagatc cgtctcggca tcaccatcat cggagcctga cgcagcttcg taatcctttg
     1741 acaaggttcc ggcaccgttg ggctcggggg cgacagacca tttgaccttg cgagcctggt
     1801 tgcggcggtg ccgtagcctt tcttcaagcg tgcggacgcg cgcctcggcc cgagcggcac
     1861 gctgtgtcgc actcgcgaaa cgaatctgca gggtcgcaat cgcatcggaa tcggaggagc
     1921 tggttgcgtc gatcaccttg ggtctgagcg cccgctcgag gcgctgctcc agcgcgctca
     1981 actcctcgcg atggcgtttt tcgagggccc gacagcgatg ctcgaggttc tcgacctttt
     2041 ccagcaacct ttcccgttcc gagagcgccc tgtagaacag cgcatgcacg tcggcgtaaa
     2101 atccggcccg gatctcgtcc gtcgcacaag gatgtgagag gaaaggccag aacagggccg
     2161 ccaacccgcg ccccttgctc cacgcctcct gccacttggc ttcgagtgcc tcaccagtcc
     2221 gcgtcccgcg caaagcaacg attttggttg cgaacttttg gtcgagagct ttctcgatca
     2281 gctccgatag cgcattgcga gtggtgcaag cgtcgcacag gtggcgatgg atgtcatagt
     2341 agcgagcctc tgcaggtatc ccgacccgag cacgccgcaa cagccgccga aactcgtcgt
     2401 cagtgaatga gagcgccagc agtgctccat gaatccctcg atgcagatcc cagaggcgcc
     2461 atcgcttgcg cggtcgtctt tgagcctcct cgcttgctgg gtcgagcagg ccgaccgcct
     2521 caaacccggt gccatctgcc gtcattgatc atgcccaact gatcgactaa ggatcagcca
     2581 ctgcttagca ttagactgca atttttacaa cgctgccgtc ttcgggttcc tcttcctgtc
     2641 agagatagcc gaggcgtcga agcttggttg ccgttgcccg atggagccgc cgtctttaga
     2701 agtttggagc cattcatctg gacaaaccat ggacacgcac gattttccag acctgatgac
     2761 tgtcagcgag gtggcggatt acctgcgcgt gcgggaacgc accatctatg agctggtccg
     2821 gacacagcgc atcccgagct gcaagctttc cggcaagctg ctgttcccga agcggctgat
     2881 cgagctttgg gtggcacagt ccgccgacta tccgcaagcc acggtgcatc tttccgcgcc
     2941 accccccgtg atcgccggca gccacgatct cctgctcgaa tggagcgtgc gcgaatccaa
     3001 ctgcgatctc gccatcctcg ttggcgggag caccgccgga ttgcaccggc tgctggccgg
     3061 tcaggccgtt gcctgcggcc tccatctcat cgaccccgaa tccggcgaat acgatgcaag
     3121 cgccctgaca aggaccttgc cgggcctcga cttcgtcgtg atcgagtggg caaggcgcct
     3181 gcagggcctc gtcgtcgcct ccggcaatcc cctgaagatc ggctctattg ccgaccttcg
     3241 taagcggcgc gttcggatcg ctacccgaca gccgggctct ggcaccgcca ttctcttttc
     3301 aaagctcctt gcagatgccg gtctcacaat cgaggagctg gacatcattg gccggccgat
     3361 gagcagccat accgacgtcg ccgatgcgat ccgacacggc aaggcagatg ccggtctggc
     3421 cattgcgtct gtcacccggg aggaaggcct cgacttcgtg cctttgcatt gggagcgcta
     3481 tgatctcgtc gtccgcagag tcgaatattt cgagccaccg ctgcaacgcc tgttcgcctt
     3541 cacgcgcacc ga
//
LOCUS       DASVDU010000080         7447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1656954, whole genome shotgun sequence.
ACCESSION   DASVDU010000080 DASVDU010000000
VERSION     DASVDU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656954"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /gene="ilvC"
                     /locus_tag="VF226_05170"
     CDS             complement(<1..903)
                     /gene="ilvC"
                     /locus_tag="VF226_05170"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX7073412.1"
                     /translation="MRVYYDRDADINLIKTKKVAIVGYGSQGHAHALNLRDSGVKEVA
                     IALRKGSASAAKAQKDNFKVMEVAEAAKWADVVMMLTPDELQADIYKDHLAANMKQGA
                     AIMFAHGLNVHFSLIEPREDLDVGMVAPKGPGHTVRSEYQRGGGVPCLLAIAQDKSGN
                     AHDLFLSYASAIGGGRAGIIETTFKEECETDLFGEQVVLCGGLVELIRAGFETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEFGEYMSGPRIITKETREEMK
                     RVLKDIQTGAFTSEWIRECKAGQPK"
     gene            complement(990..1355)
                     /locus_tag="VF226_05175"
     CDS             complement(990..1355)
                     /locus_tag="VF226_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX7073413.1"
                     /translation="MSIAGAGELPDAGDIVWVDLDPVRGTEQAGKRPALVLTSRPFHE
                     NSRRAIICPITSNRAPWPTKVLLPDGLQIKGAILVDQIRSLDRRTQGFRPAGRVPEYV
                     LEEVRARLKELIGISMLPQ"
     gene            complement(1333..1611)
                     /locus_tag="VF226_05180"
     CDS             complement(1333..1611)
                     /locus_tag="VF226_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7073414.1"
                     /translation="MQVRVSRWGNSLAVRLPKAVAENLRLEEGQTVDLEIEDGSVRLR
                     PAGRPKYPTLAEMVAEMKRLGPENEPPTVDWGPDVGAEIIDHEYSRRG"
     gene            complement(1759..2406)
                     /locus_tag="VF226_05185"
     CDS             complement(1759..2406)
                     /locus_tag="VF226_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073415.1"
                     /translation="MLARGCWLAALVAITVGGCSYEHALGVDHYSVFKVDPPHGSTVS
                     VCSAYGCRKQTKFTFSQADLAEIKKLMEKTKKADTPYEERRAIAYAIGWMERRTGEVV
                     GTKADRPGMDFAASGDPTQQDCVDEATNTTSYLTVLQNAKLLKHHTVGQPFSKDNFLR
                     GVSGWTHWTATIYENKSGQRWAVDSWIYENGENPAIVNADEWYVASINELPKATQ"
     gene            2552..3970
                     /gene="trmFO"
                     /locus_tag="VF226_05190"
     CDS             2552..3970
                     /gene="trmFO"
                     /locus_tag="VF226_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX7073416.1"
                     /translation="MATAKPIHVIGGGLAGSEAAWQIARAGVPVIIHEMRPIRGTEAH
                     KTDGLAELVCSNSFRSDDWETNAVGLLHEEMRRAGSLIMSAADRHKLPAGGALAVDRD
                     GFSAEVTERLTAQPLVQIERGEVAGLPPEEWDSVIIATGPLTSPALSQAILDATGEDA
                     LAFFDAIAPIVHYDTIDMSIAWRQSRYDKPGPAGTGADYINCPMTKAEYEAFVDALLA
                     GEKTSFREWETNTPYFDGCLPIEVMAERGRDTLRFGPMKPVGLSNPHRGGERPYAVVQ
                     LRQDNALGTLWNMVGFQTKLKYGEQVRIFRMIPGLQNAEFARLGGLHRNTFLNSPKLL
                     DGLLRLKTAPRIRFAGQVTGVEGYVESAAIGLLAGRFAAAERLGRQPLPPPAATALGA
                     LLSHITGGHLAEERIEAGARSFQPMNINFGLFPQPDLPADGPKLKGKERGRAKKRSIA
                     LRALREIDGWLAAGAVVRSAAE"
     gene            complement(3999..5933)
                     /locus_tag="VF226_05195"
     CDS             complement(3999..5933)
                     /locus_tag="VF226_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073417.1"
                     /translation="MLRNGVRAGLLLLALCVGQRAFAIETAQIHPQIEWRVENPFRFF
                     TDPTDTQVHRATFLSLSDEEHETPVLSAERELSKKHADGWASIVLGRTCFDVQRHLYV
                     CPDGTDYINPRSHSIIAELKGIPDAGLIDCTWLTAPYGPRAPRGQAVTRPCSEPVRLD
                     VPYPNGARISVEIGGREITDTEAVVTDLFIVGMGDSFASGEGNPDLPIRFSRERSVNY
                     GTGLLGSDLVGYPARIGGWRQIGDERFIQENARWHDQACHRSLYSHQLRAALQLSIED
                     PHRAVTFVGVSCSGAEITQGLFLRYKGNEWVPNPPRLSQISAVAEAQCGGKRPRRHDL
                     PEAYHLNGRVPELRGLTLLKCDLEYARKIDLLMISIGGNDVGFSRLVADAVLSDKSLL
                     KWLGGWLGEIEEAAAAKDQLGTLYLRYMALDRAIRNILHIPWEENDRVLLTAYPGLAL
                     LEDGNTVCPSGRAGMDILQDFKLSAEKAREGSILAERLNEVMRRAAREHGWTFVESHR
                     KAFLGRGICAGWSDAAFSTADDLRLPRKIKGVWRPYNPADYRPYASRQRWFRTPNDAF
                     LTGNFHVPATLLQKVLQVNNSWFQLVLASTYSGAFHPTAEGQAAIADAVVERARRVLA
                     KYARRSSAQEINLEEPRFNP"
     gene            complement(5989..6858)
                     /locus_tag="VF226_05200"
     CDS             complement(5989..6858)
                     /locus_tag="VF226_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX7073418.1"
                     /translation="MSETGTAGVPVAHEDVVRASARAWEMDRYLSALLSPRPVRDDLI
                     ALAAFAGEIERIPAFVSEPMMGEIRLQWWREALENPDLAIRSGHPVADAVRATAARRR
                     LPMGLLFGFIDAQSTLLHDEPMTDDHALAMHVAKTEGALFELALRILGATDELTHAAA
                     LAAGQAYGIARLLVELPAAWAQGRVLIPVSRLSEFGLSLAQVQAGAPADRLAPVLAGL
                     VEESRRHLAEARIYSRRLNRAQHKAFLPLAVVEPYLRAFQRGRRDPLREPLEIAPLSR
                     VWALFRSSVTGRF"
     gene            complement(6860..>7447)
                     /locus_tag="VF226_05205"
     CDS             complement(6860..>7447)
                     /locus_tag="VF226_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073419.1"
                     /translation="ETAGRALAAGAVLTLAILILWLALAGADLLGFLSFLLRWIHVGA
                     AILWVGMIWFVNVIQLSALQQADEAGRRTIMSVIVPRVAHSFRHASHLTLVTGLLLLV
                     TTGYLFDTLVFSTPVYVPTARTVLLWAGVAGGVVMWGFVHFIIWPNLRIVLGETAAEA
                     DAKERARAEVRTYARLNLLLAVPVTFAMVAAPHLY"
BASE COUNT         1340 a         2444 c         2351 g         1312 t
ORIGIN      
        1 cttcggctgg cccgccttgc actcgcggat ccactcgctg gtgaaagcac cggtctggat
       61 gtccttcagc acgcgcttca tctcctcgcg cgtttccttg gtgatgatcc gcgggcctga
      121 catgtactcg ccgaactcgg ctgtgttgga aatcgagtag ttcatgttgg cgatgccgcc
      181 ctcgtagatg aggtcgacga tcagcttcac ctcgtgcaga cactcgaagt aggccatctc
      241 gggcgcatag cccgcctcga ccagcgtctc gaacccggcg cggatcagct cgacgaggcc
      301 accgcagagc acgacctgct cgccgaagag atcggtctcg cactcctcct tgaaggtcgt
      361 ctcgatgatg ccggcgcggc cgccgccgat cgcggatgcg taagaaagga acaggtcgtg
      421 cgcgttgccg ctcttgtcct gggcgatggc gagcaggcac ggcacgccgc cgccgcgctg
      481 gtactcggag cgcaccgtat ggccggggcc cttcggcgcc accatgccga cgtcgaggtc
      541 ctcgcgcggc tcgatcaagc tgaagtgaac gttgagaccg tgcgcgaaca tgatcgccgc
      601 gccctgcttc atgttggcgg caaggtggtc cttgtagatg tcggcctgca gctcgtcggg
      661 cgtcagcatc atcacgacgt cagcccactt cgccgcctcg gcgacctcca tcaccttgaa
      721 attgtccttc tgggccttgg ctgccgaggc cgagcccttc ctgagcgcaa tcgccacttc
      781 tttgacgccg gaatcgcgca ggttgagcgc atgcgcgtgc ccctggctgc cgtagccgac
      841 gatggccacc ttcttggtct tgatcaggtt gatgtccgca tcacgatcgt aatagacgcg
      901 catgactgtt cctcttatcg cgtttcaaat catctgcttc gggggcgact tccgttggcc
      961 gcagaccgaa atcctgtgcg cccgggaccc tactgaggga gcatcgaaat gccaatcaat
     1021 tccttaagcc gggcgcgtac ttcctcgagc acatactcgg gcacacgtcc agcgggccga
     1081 aagccctgtg ttcgtcgatc caggctgcga atctgatcga ccagaatggc gcctttgatc
     1141 tgcagcccat cgggcagcag caccttggtc ggccatggtg cgcggttgga tgtgatcggg
     1201 cagattatgg ccctgcgcga attctcatgg aatggacgtg acgtgagcac gagcgctggc
     1261 cgcttaccgg cctgttcggt gcctcggacg gggtcgaggt cgacccagac gatgtcgccc
     1321 gcatcgggca gttcacccgc gccggctata ctcatgatct atgatctcag ctccgacgtc
     1381 gggcccccaa tcgactgtgg gtggctcgtt ctccggccct aaacgcttca tctcggccac
     1441 catctcagcc agcgtcggat acttgggccg accggcaggc ctcagccgca ctgagccgtc
     1501 ttcgatttca aggtcgacag tttgcccctc ttcgagacgg agattttcag cgaccgcctt
     1561 gggcagccgc acggccaaac tgttgcccca tcgcgaaact cggacttgca tagtcacact
     1621 ccttctccgg atatacatag tatatcccga gcttgcacag caagctccag ttggacgcgt
     1681 ccagcaaggt cacgccgagg gtcggtcagg ttggagatgc cggatagggg tgcctgacgt
     1741 caggccacaa tcgcggcctc actgggtcgc cttcggcagc tcgttgatcg aggcgacgta
     1801 ccactcgtcg gcgttgacga tcgccgggtt ctcgccattc tcgtagatcc agctgtcgac
     1861 cgcccagcgc tggccgctct tgttctcgta tatcgtcgcc gtccagtggg tccagccaga
     1921 gacgccgcgc aggaaattgt ccttcgagaa gggctggccg acggtgtggt gcttcagcag
     1981 cttggcattt tgcaggacgg tgagatagct cgtcgtattg gtcgcctcgt ccacgcagtc
     2041 ctgttgggtc ggatcacccg aggctgcaaa atccatacct gggcgatcgg ccttcgtgcc
     2101 cacaacctcg ccggtccggc gctccatcca gccgatggca taggcgatag cgcggcgctc
     2161 ctcatatggc gtatcggcct tctttgtctt ttccatcagc ttcttgatct cagcgagatc
     2221 agcctgggag aacgtgaact tggtctgctt gcggcagccg taggcgctgc agacggaaac
     2281 ggtcgagcca tgtggcggat cgactttgaa gaccgaataa tggtcgacac ccaaggcgtg
     2341 ctcataggaa cagccgccaa ccgttatggc gaccagtgcc gccagccaac acccccgtgc
     2401 gagcatttcc cttggcgccc cgacaagttg taaacaggtt ccggccgact tttgctccaa
     2461 gccgcgccat ggctccttag tatcctactg attcggtaaa ggacacccag cgcattgagc
     2521 ccgcagagct tttgcagtac gttgcagcgc gatggccaca gcgaagccca ttcacgtcat
     2581 cggcggcggg ctggccggct ccgaggccgc ctggcagatc gcccgcgccg gcgtgcccgt
     2641 catcatccac gagatgcgcc cgatccgtgg aacggaggcg cacaagacgg acgggctggc
     2701 agaactcgtc tgctccaatt ccttccgctc ggatgattgg gagacgaacg cggtcgggct
     2761 cctgcacgag gagatgcggc gcgccgggtc gctcatcatg tccgcggccg accgccacaa
     2821 gttgcccgcc ggtggcgcgc tcgcagtcga ccgcgatggc ttttccgccg aggtgacgga
     2881 gcgcctgacc gcccagccct tggtgcagat cgaacgcggt gaggtcgcag gactgccacc
     2941 ggaggagtgg gacagcgtca tcatcgccac cggcccgctc acctcgccgg ccctgagcca
     3001 agccattctc gacgcaacgg gcgaggacgc gctcgccttc ttcgatgcga tcgccccgat
     3061 cgtgcattac gacacgatcg acatgagcat cgcctggcgg cagtcgcgct acgacaagcc
     3121 cggtccggca ggcaccggcg ccgactacat caactgcccc atgaccaagg ccgagtacga
     3181 ggccttcgtc gacgccctgc tcgcgggtga gaagacctcc ttcagagagt gggagacgaa
     3241 cacgccctac ttcgacggct gcctgccgat cgaggtcatg gcggagcgtg gccgcgatac
     3301 gctccggttc ggaccgatga agcccgtcgg cctctccaat ccgcacagag gcggcgagcg
     3361 gccgtatgcc gtcgtgcagc tccggcagga caacgcgctt ggcacgcttt ggaacatggt
     3421 cggcttccag acgaagctga agtacggcga gcaggttcgg atcttccgca tgatcccggg
     3481 cctgcagaat gccgagttcg caagattggg cggcctgcac cggaacacat tcctgaacag
     3541 ccccaagctg ctggacgggc tcctgaggct caagacggcg ccgcggatac gctttgccgg
     3601 acaggtgacc ggcgtcgagg gctacgtgga aagcgccgcc atcggtctcc ttgccggacg
     3661 gttcgcggct gcggaacggc tgggtcgaca gccgctgccg ccacccgctg caacagccct
     3721 aggggcactg ctcagccata tcaccggagg ccacttggcg gaggagcgca tcgaggcggg
     3781 cgcccgctcg ttccagccga tgaacatcaa cttcggtcta ttccctcagc cggacctgcc
     3841 cgcagacggc ccgaagctca aggggaagga gcggggacgt gcgaagaagc gatcaatcgc
     3901 cctgcgagcg ctacgggaga tagacggctg gctcgcggcc ggcgcggtcg tccggtcggc
     3961 cgccgaatag cggccccagc cctcttttct ggtggcagct acggattgaa ccgcggctcc
     4021 tcgaggttga tctcctgcgc gctcgacctg cgggcgtatt tcgccagaac acgacgcgcc
     4081 ctctcgacaa cagcatccgc gatcgctgcc tgcccttcgg ccgtcggatg gaaggcgccg
     4141 gaataggtcg atgccagcac gagctggaac caggaattat tgacctggag caccttctgc
     4201 agcagcgtcg ccggcacgtg aaagtttccg gtcaggaaag cgtcgttggg cgttctgaac
     4261 cagcgctgcc gcgaggcata gggacggtag tcggccggat tgtagggccg ccagacgcct
     4321 ttgattttcc gcggcagcct cagatcgtcg gcggtcgaga aggccgcgtc gctccaaccg
     4381 gcgcagatgc cgcggccgag aaacgccttg cggtggctct cgacgaacgt ccagccgtgc
     4441 tcacgcgcgg cccggcgcat cacctcgttg agccgctccg cgagtatact gccctcgcgg
     4501 gctttctctg cgcttagctt gaaatcctgg agtatatcca taccggccct gccgctgggg
     4561 cagacggtgt taccgtcctc gagcagtgcg agcccgggat aggccgtcag cagaacccgg
     4621 tcgttctctt cccagggaat gtgcagaatg ttccggatcg cccgatccag cgccatgtag
     4681 cgcaggtaca gcgtcccgag ctgatccttt gccgctgcgg cttcttcgat ttcgccgagc
     4741 cagccgccca gccacttcag cagagacttg tcgctcagca ccgcatcggc aacgagccgc
     4801 gagaacccga cgtcgttgcc gccgatcgag atcatcagca ggtcgatctt gcgtgcatat
     4861 tcgagatcgc atttgagcag cgtcagccct ctgagctcgg ggacgcggcc gttgagatga
     4921 taggcttcgg gcaggtcgtg gcggcgcggc cgttttccgc cacactgggc ttcggccacc
     4981 gcggagatct gcgacagcct cggcggattc ggcacccatt cgttgccctt gtaccgcagg
     5041 aaaagccctt gcgtgatttc cgcgcccgag caggacacgc cgacgaacgt caccgctcga
     5101 tgcgggtcct cgatcgaaag ttgtagggcg gctctcagct ggtgcgagta aagcgagcga
     5161 tggcaggcct gatcgtgcca gcgcgcgttc tcctggatga accgttcatc gccgatctgg
     5221 cgccagccgc cgatacgggc cggatagccg acgaggtccg acccgagcag ccctgtgccg
     5281 tagttgaccg accgctcacg cgaaaagcgt atgggcaggt ccggattgcc ctctccggag
     5341 gcgaagctgt cgcccatgcc gacgatgaag aggtcggtca cgaccgcttc cgtgtccgtg
     5401 atctcgcgcc cgccgatctc cactgagatc cgcgcgccat tgggataggg cacatccagc
     5461 cgcaccggct cgctgcacgg gcgcgtcacg gcctgcccgc gcggcgcccg aggaccatag
     5521 ggcgcagtca gccaggtgca gtcgatgagc ccggcatcag ggatgccctt cagctcggcg
     5581 atgatgctgt gcgagcgcgg attgatgtag tcggtgccgt cggggcaaac gtaaagatgc
     5641 ctctggacgt cgaagcaggt ccgaccgagg acgatggacg cccagccgtc ggcgtgtttc
     5701 ttcgacagct cccgctcagc cgaaagcacc ggcgtttcgt gctcctcgtc cgacagggac
     5761 aggaacgtcg cccggtggac ctgagtgtcc gtcggatcgg tgaagaagcg gaaaggattc
     5821 tcgacccgcc attcgatttg cggatgaatc tgcgcggttt cgatcgcaaa tgcacgctgg
     5881 ccaacgcaca gcgccagcag aagcaagcct gccctcaccc cattgcgcag catgtctgtt
     5941 ccccgtgagc ggccagttcg caatcgggtc gcccgctccg cggtgccgtc agaaccgacc
     6001 ggtcacgctc gatcggaaca aggcccagac ccgactgagc ggcgcgatct cgagcggttc
     6061 gcggagtgga tcgcgccgtc ctctctgaaa agcccgcaaa tagggctcta cgacggcaag
     6121 tggcaaaaat gctttgtgct gtgcacggtt gaggcgtctg ctgtagatcc gcgcttcagc
     6181 caaatgcctg cgcgactcct cgacgagccc cgccaagaca ggagcgagcc ggtccgccgg
     6241 agcaccagcc tggacctggg caaggctcag cccaaactcg gacagtcgag agacggggat
     6301 cagaacccgg ccctgtgccc aagcagccgg caactctacg agaaggcgtg cgatgccata
     6361 ggcttggccc gccgccagtg cggctgcatg ggttaattcg tcggtggccc ccagaatgcg
     6421 cagcgccagc tcgaaaagag cgccctcggt cttcgccacg tgcatagcca gcgcgtgatc
     6481 atccgtcatg ggttcatcgt gaagaagcgt cgactgggca tcgatgaagc cgaacagcag
     6541 acccatcggc agcctgcgcc gcgccgcagt ggcccgaaca gcatccgcga cgggatggcc
     6601 gctgcggatc gcaagatccg ggttctccag cgcctcccgc caccactgca ggcggatctc
     6661 gcccatcatt ggctcgctga cgaacgccgg aatgcgctcg atctccccgg cgaaggccgc
     6721 cagggcgatg agatcgtccc gcaccgggcg cggcgacagc agcgcagaca ggtagcggtc
     6781 catttcccag gcccgcgccg aagcgcggac gacgtcctcg tgcgcgaccg gaacgccggc
     6841 tgttcccgtc tcgctcacgt cagtagaggt gtggcgccgc aaccatggcg aaggtcaccg
     6901 gtacggcgag gaggaggttg aggcgcgcat aggttctgac ctcggcccgg gcgcgttcct
     6961 tggcgtccgc ctcggccgcc gtctccccga ggacgatccg caggttgggc catatgatga
     7021 agtgaacgaa gccccacatg acgacgcctc ccgccacgcc ggcccataac aggacggtgc
     7081 gcgccgtcgg gacatagacc ggcgtcgaga agacaagcgt atcgaagaga tagccggtgg
     7141 tcaccagcag aagcaggccg gtcaccagcg tgaggtgcga ggcgtgacgg aaggaatgcg
     7201 ccaccctggg cacgatgacc gacatgatgg tgcgccgccc tgcctcatcc gcctgctgca
     7261 gggctgagag ctggatgacg ttgacgaacc agatcatccc gacccagagg atggcagcac
     7321 cgacgtgaat ccatcggagg aggaatgaga ggaagccgag caaatcggcg cccgccagcg
     7381 ccagccacag gatcaggatg gcaagcgtga gcacggcgcc ggccgccagc gcacgcccgg
     7441 cggtttc
//
LOCUS       DASVDU010000081        12297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_922894, whole genome shotgun sequence.
ACCESSION   DASVDU010000081 DASVDU010000000
VERSION     DASVDU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922894"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF226_05210"
     CDS             <1..313
                     /locus_tag="VF226_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073420.1"
                     /translation="REMFMEREIHPEAKARVSEVIRRAAERGQHEVMAIRFASAWCTD
                     KGRAINNAEPDWPKTLPGFAARAYGFYEKELRPHGYKLRAEVLDFPGGMPGDIGIFLR
                     W"
     gene            389..1795
                     /locus_tag="VF226_05215"
     CDS             389..1795
                     /locus_tag="VF226_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HEX7073421.1"
                     /translation="MDALENRPFDDITIGDSASVTKTLTRDDIALFAVVSGDLNPTHL
                     DDTSAEASPMRRVVGHSLWGGGLISGLLGTRLPGPGTVYLAQNLRFHHRIAPGDTLTA
                     KVTVREKRPKANVVVLDCSCTNQDGEPVLSGTAEVVAPTAKMSVPCGDLPAVSLRRHD
                     AYERLIERCRDLEPLSTAVAHPCDESSLSAAVDAARSGIIAPVLVGPANKIRDAAQQF
                     GLDISAFPLIEAAHSHDAAEKAVALVRTGQAEALMKGSLHTDELMAAVVKRETGLRTQ
                     RRISHCFLMDVPNYARPLIITDAAINIYPSLEDKVDIVQNAIDLARALGLERPCVAIL
                     SAVETVNPKISSTLEAAALCKMAERGQITGGVLDGPLALDNAISEEAARIKRIESPVA
                     GHADILLVPDLEAGNMLAKNLTFLANADAAGIVLGARVPIILTSRADSVQTRLASCAV
                     ATLCARSRREATAAAAAE"
     gene            1802..3028
                     /locus_tag="VF226_05220"
     CDS             1802..3028
                     /locus_tag="VF226_05220"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX7073422.1"
                     /translation="MVEVILALNAGSSSLKFAIFGLVDPGDPALIAKGQIEGIGKRPH
                     LVARDANGQPVADEHLSAGKVPDHGQAMVDLTAWLRTRYAGMQLRAIGHRVAHGGPGL
                     AAPMLIDGELVEWLASYIPLAPLHQPHNLAAINAGRQRLPDLPQVACFDTAFHRGHSE
                     VVDRFALPDSFYREGIRRYGFHGLSYEYIARVLPSVAPSIASGRVVVAHLGSGASMCA
                     IQNGRSVDSTMSFTALDGLPMGTRCGALDPGVLLYLIGQKGFDASRLERLLYRESGLL
                     GLSGESNDARDLLVSSSPMATLALDYFVHCVCRELGALSATLQGLDGIVFTAGIGENS
                     AVIRARICEQASWLGLRLDEEANLASGPQITTPDSDVSVWVIPTDEEKMIALHTLATL
                     RERATAAHNPARITAD"
     gene            3130..3912
                     /gene="fabI"
                     /locus_tag="VF226_05225"
     CDS             3130..3912
                     /gene="fabI"
                     /locus_tag="VF226_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX7073423.1"
                     /translation="MLDFTKLRSLEGKRGLVIGIANDKSIAFGCAKAFRHYGAELAVT
                     YLNEKAKPYVEPLARQIEASIVMPLDVQVGGQMEALFSAIEERWGRLDFALHAIAFAP
                     KEDLHGRVLDCSAQGFHVAMGVSCYSFIHMARLAAPLMTDGGTLFTMSYYGAEKVVGN
                     YNIMGPVKAALEATVRYLAAELGSRGIRVHAISPGPLATRAASGIAHFDELLNQAAKR
                     APAGRLVTIEDVGVACAGLATEGARLITGDTLYVDGGLHVLG"
     gene            4027..4656
                     /locus_tag="VF226_05230"
     CDS             4027..4656
                     /locus_tag="VF226_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX7073424.1"
                     /translation="MQLGTVDIATLLLVTVGPLKALIVFGTLTARADSDFRRQVAIRT
                     VAVATIVTLLFVLAGEFLLQIFHITLPALKIAGGIILLLFAIHMVMGEAGKKEDIKMP
                     SPDIAIFPLAMPMMATPQGLVAITVIVAGRQSYSDVLVVVVILLAIMVFNLVCMLSAD
                     RIISAIGPAALQVIAKIAGLLLTALAVQLMISAFRDLGLIERVIGATGH"
     gene            4653..5747
                     /locus_tag="VF226_05235"
     CDS             4653..5747
                     /locus_tag="VF226_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX7073425.1"
                     /translation="MTTDEIQAEISERKPGRRWLAPVLAVCAAIFVYNLIADRLTPYT
                     SKATVQAYLIGIAPEVAGTVIDVGVVDNQRVKAGDVLFRIDPRRYEAALREAQAAVDR
                     AGQSIGANTAGVAAAEAALTDARAQYANAREQATRIFELVKRGIYAKARGDQMQRLLE
                     GAQAGVRQAEAQLEQARQQLGPKGESNPELQAALSALEQARLNLNDTVMVSPANGRVT
                     NLRLIGGQFVAAGQPALTVIDTQAIWISANFRENNLGNMSQGNPAEIVLDVRPGEVFP
                     GVVESIGWGVDVGETTAGSGLPVIRNPSGWLREAQRFPARVRFETDAYPRGIRYGAQA
                     NIIVYTTDNRVMNGLAWLWIRAVAWLSYLY"
     gene            5692..6870
                     /locus_tag="VF226_05240"
     CDS             5692..6870
                     /locus_tag="VF226_05240"
                     /inference="COORDINATES: protein motif:HMM:NF022616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2955 domain-containing protein"
                     /protein_id="HEX7073426.1"
                     /translation="MVSHGSGSAPSLGFPTSTERQETAEAAPRLEERPVPAERHQAAH
                     AERTRGLGLRVGFAVTAGFLVSELLDWELSFLTPVLAVQLLAAAPAMPSFRQGLAIIV
                     VFGGTTGAALVASELFANMPVLFTALLALVTLWAFYLQARRQQQLLSMLLLVSFTIVP
                     VVAIREPTAAAVVAFSVFRSGITVVLLIWLAFTLFPSDPIPEMPGKPIRLPLRAREPA
                     MREALVDTAVIMPVLVAALTFTTTAVIFIVMTISAVLSQRAFAQASQTALGLFLGNLL
                     GGATAIVAYQLLRAVPSLGFFTALLLFIGLSYGFAIERGGTRSHLLVVALVTFLIVLG
                     VGISPFLDEPTASLSTRIVNLGFACAYAFLALALIQALRRPEQPRWPAASSPDAPGAA
                     "
     gene            6926..8269
                     /locus_tag="VF226_05245"
     CDS             6926..8269
                     /locus_tag="VF226_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073427.1"
                     /translation="MAKRYRPKGDAGIGALTRVLFSAVLSFSICASAQASEDVQELIR
                     HQFEIYDPAYGAHREQYGERLKAMSEAIAEAQANGRNLHCTQQMYLEAKWLYRYTAHW
                     DRLADKLTRIEQSLDDPDQSFAAEQSPVDGMWGICYEEWFMRLAATVDGLENLAARGE
                     RPRYQLRSAGKFDTGKKLVTRMQGLLISDIAHTGVDNRGELSSLITSIAQGAFKPLLR
                     DMLVKSADLRISSGLDSVTEAFRFFLSGAQDPTTGYWGAWYIVDGKIHKTSDLSMTYH
                     VIAYTNGRVERWPQIIATTKAIEALPYPYGWRHNGKYNNHNLYDVAKIYKFAWPHMSK
                     AEHLEIAKQIDSMIHWSLANTLGPDDQFLHDPTFSDSLTDEYYFGVSFLDVVGYWKQD
                     RRFWTPSLVDKEGAALCCRLKHRMTELDLEGWAANGAMRKLERNCSQCPAPNAEE"
     gene            complement(8487..9089)
                     /locus_tag="VF226_05250"
     CDS             complement(8487..9089)
                     /locus_tag="VF226_05250"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073428.1"
                     /translation="MLSRRRVTIGLAGFAALLARGSSRAEQADLDPKRFIEQLGMEAV
                     EALTGPELSESERERRFRGMLDRSFDLPVIGRFVLGRYWRTASEAEREEFLRLFEAFL
                     VKSYAARFADYAGEQLQVQGVRQEQGYAIVRSTVIRPGAENVQVDWRLRGRDGAFKVV
                     DIVVEGISMAVTQRADFGSVIQSSGGEFSGLIELLRRKTG"
     gene            complement(9387..10325)
                     /gene="arcC"
                     /locus_tag="VF226_05255"
     CDS             complement(9387..10325)
                     /gene="arcC"
                     /locus_tag="VF226_05255"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX7073429.1"
                     /translation="MRIVVALGGNALLKRGEPMTADAQRRNVKVAAEALAPVARDHQL
                     VISHGNGPQVGLLALQGASYKPGEAYPLDVLGAETEGMIGYMIEQELGNLLPFEVPFA
                     TLLTMIEVDPDDPGFRSPTKFVGPVYDKAGADRIAKEKEWIFKADGDKWRRVVPSPEP
                     KRIFEIRPIRWLLDRGTIVIAAGGGGIPTMYERDADRRLIGVEAVIDKDLASELLARE
                     LDADLFIMATDASGVYVNWGEPDAKAIHRASPAAMREYQFPAGSMGPKVDAACRFAQA
                     TGKTAAIGALAEIPAIVAGDKGTLVSADHEGIVWHH"
     gene            complement(10331..11335)
                     /locus_tag="VF226_05260"
     CDS             complement(10331..11335)
                     /locus_tag="VF226_05260"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037116981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7073430.1"
                     /translation="MSFNLRNRSLLTVQDYTQREFRYLLDLARDLKRAKYARTEQRHL
                     ADKEICLIFEKTSTRTRCAFEVACHDQGANVTYLDPVGSQIGHKESYKDTARVLGRMY
                     DAIEYRGASQEGVEQLAKYAGVPVYNGLTDEYHPTQMLADIMTMREYSDKPIHDIKYA
                     YVGDTRSNMGHSLMIAGCLMGMDVRVCGPKTLWPSEEYRGIAEDLTRRCGAKLTVTDD
                     PKTAVQGVDFIHTDVWVSMGEPKEVWKERIELLKPYQVNAELMEATENPQVKFMHCLP
                     AFHDTETTVGRQIAEAYGMRNGLEVTNDVFESERNIAFEQAENRLHTIKAILVATLGD
                     "
     gene            complement(11370..>12297)
                     /locus_tag="VF226_05265"
     CDS             complement(11370..>12297)
                     /locus_tag="VF226_05265"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037116983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX7073431.1"
                     /translation="AMRPWLDEMPAKLLAEHLIGGIAVDDLPENGVKRLLGDAFGGTD
                     FVIPPIPNTLFQRDPSCWIYAGVTCNPMFWPARKPETLLQRAVYKFHPLFKKADFRIW
                     WGDSDEDFAYATLEGGDVMPVGKGLVLIGMGERTSREAVMRVACELFRNEAASRVIAC
                     LMPKSRAAMHLDTVFTFCDRDVVTVFREVVDQIACYSVYPGSKPVGIEVRREERPMLQ
                     VVQEALGLNTLRAVETGGDAYDAQREQWDDGNNVVALEPGVVVAYDRNVHTNTLLRKA
                     GIEVITVRGAELGRGRGGGHCMTCPVSRDPAY"
BASE COUNT         2202 a         3931 c         3818 g         2346 t
ORIGIN      
        1 gcgcgaaatg ttcatggagc gcgagatcca tccggaagca aaggccaggg tctcggaggt
       61 catccgccgc gccgccgagc gcggccagca cgaggttatg gccatccgct tcgccagcgc
      121 gtggtgcacc gataaggggc gagcaatcaa caacgcggaa ccggattggc cgaagaccct
      181 gccgggcttc gccgcacgcg cctacggctt ctacgaaaag gagctgcggc cgcacggcta
      241 caagcttcgc gccgaagttc tcgactttcc gggagggatg cctggcgaca tcgggatctt
      301 tctacgctgg tagcctagca gcgagccgtg agaccggctt tttcctccga ccatgaaaca
      361 gccgcggccc gaatagggaa caacgaagat ggacgcgctg gaaaaccggc cattcgacga
      421 catcacaatc ggcgactcag ccagtgtgac gaagacactg acgcgtgatg acatcgcctt
      481 gttcgcggtt gtctcgggtg acctcaaccc gacccacctc gacgatacct cggccgaagc
      541 gagcccaatg cggcgcgtgg tcggccacag cctatggggt ggcggtctga tctcaggcct
      601 gctcggaacc cggctacccg gccctggaac ggtgtacctc gcccagaacc tgcgcttcca
      661 ccatcgaatc gcgccgggag atacgctaac cgccaaagta acggtccgcg agaagcgtcc
      721 gaaggcgaac gtcgtggtgc ttgattgctc gtgcacgaac caggacggag aacctgttct
      781 cagcggaacg gccgaagtcg ttgcacctac ggcaaagatg agtgtgccgt gcggcgatct
      841 gcctgcggtt tcgctgcgcc ggcatgatgc ctatgagcgc ctgatcgaac ggtgccggga
      901 cctcgaaccg ttgagcactg ccgtggccca tccgtgcgac gaatcttcgc tttccgccgc
      961 ggtcgacgcc gcgcgatcgg gcatcattgc gccggtcctg gtcggaccgg caaacaagat
     1021 ccgcgacgcc gcgcagcaat tcgggttgga catttcagca tttccgctaa tcgaggccgc
     1081 gcacagccat gacgccgccg agaaggcagt cgcattggta cgcaccggac aggccgaagc
     1141 gctgatgaag ggcagcctgc acaccgacga gttgatggcc gccgtggtca agcgcgagac
     1201 ggggctgcgg acacagcggc ggatcagcca ttgcttcctg atggacgtgc cgaactatgc
     1261 gcggccgctg atcatcaccg acgccgcaat caacatctat ccgagtctgg aagacaaggt
     1321 cgacatcgtt cagaacgcca tcgacctcgc ccgggcgttg ggtcttgagc gcccctgcgt
     1381 ggcgatcctg tccgcggtgg agacggtgaa tcccaagatc tcctccacct tggaggcggc
     1441 ggcgctctgc aagatggcgg agcgcgggca gatcaccggc ggcgtgctcg acggcccact
     1501 tgccctcgac aacgccatca gcgaggaggc cgcccgcatc aagcgcatcg aatctccggt
     1561 ggcggggcat gccgacattc tcctcgtgcc ggatctcgag gcgggcaaca tgctcgccaa
     1621 gaatctcacc ttccttgcga atgccgatgc ggcgggcatc gttctcggcg cacgggtgcc
     1681 gatcatcctg acgagccgcg ccgattctgt ccaaacgcgc ctggcgtcct gcgcggtggc
     1741 caccctttgc gctcggtccc ggcgggaagc caccgcggca gccgcggcgg agtgagcggc
     1801 catggtcgag gtgatcctgg ccctgaatgc gggctcctcc agcctcaagt tcgcgatctt
     1861 cgggctggtg gatcccggcg atcccgcgct tattgcgaaa ggccaaatcg agggtatcgg
     1921 caagcggccg caccttgtcg ctcgggacgc taatggccag ccggtggccg acgagcactt
     1981 aagcgccggc aaggtcccgg accatgggca ggcgatggtg gatctgaccg catggcttcg
     2041 aacacgctat gcggggatgc agctccgcgc gatcggccat cgcgtggccc atggcggccc
     2101 tggtcttgca gcgcctatgc tgatcgatgg tgaactggtg gagtggcttg cgagctacat
     2161 tccgctggca ccgctgcacc agccgcacaa cctcgctgcc ataaatgccg gccgccagcg
     2221 gttgccggac ctgccgcagg tggcctgctt cgacaccgcg ttccaccgcg ggcattccga
     2281 ggtcgtggac cgctttgctc ttcccgattc cttctaccgt gaaggaatcc gacgctacgg
     2341 cttccatggg ctctcctacg agtacatcgc ccgcgtgctt ccgagtgttg cgccgagcat
     2401 cgcatcgggg cgggtcgtcg tggcccacct cggcagtggc gcgagcatgt gcgcgatcca
     2461 gaacggccgg agcgtcgaca gcacgatgag cttcaccgcc ttggacggcc ttccgatggg
     2521 gacccgctgc ggcgcactcg atccgggcgt cctgctctat ctcattggcc aaaaaggttt
     2581 cgatgccagc cgcttggaac gcctgctgta cagggagagc gggctactgg ggctatccgg
     2641 agagagcaac gacgctcggg atctgctcgt gagcagctcg ccgatggcga cgctcgccct
     2701 ggattatttc gtccattgcg tttgtcgcga actcggcgcc ttgagcgcga ctctccaagg
     2761 gctcgacggg atcgtattca ccgcaggtat cggcgagaac tccgccgtga tccgggccag
     2821 gatctgcgag caggccagct ggctcggcct gcggctcgac gaggaggcca atctcgcaag
     2881 cggcccgcag atcacgacac ccgactccga cgtatcggtg tgggtgatcc ccaccgacga
     2941 ggaaaagatg atcgccctgc acacgcttgc gactcttcgg gagcgcgcaa cagccgctca
     3001 caaccccgcg cgaattaccg cggactgagc atggctgtcg cgtcgcaacc tgcttaccgc
     3061 tgccctcggg cttttccgtc gcggcaatga gaggacatcg ccaccaaaga ttcggagcga
     3121 aaggcagaca tgctcgactt tacgaagctg cgtagcctcg aaggcaagag aggcctcgtg
     3181 atcggcatcg cgaacgacaa gtcgatcgcc ttcggctgcg cgaaagcgtt ccgtcactac
     3241 ggcgccgaac tggctgtgac gtatctcaac gaaaaggcta agccttacgt cgagccgttg
     3301 gcgcgtcaga tcgaggcaag catcgtgatg cctctcgacg tgcaagtggg cgggcagatg
     3361 gaggcgctgt tcagcgcgat agaagaacga tggggacggc ttgatttcgc gctccacgcg
     3421 attgccttcg cgccgaagga ggatctgcac ggacgcgtct tggactgttc ggcccagggc
     3481 ttccacgtcg ccatgggggt atcgtgttac tccttcattc atatggcgcg ccttgccgcg
     3541 cccctgatga ccgatggcgg caccctattc acgatgagct attacggcgc cgagaaggtg
     3601 gtcggaaact acaacatcat ggggccggtc aaggcggcgc tcgaggccac ggtgcgctac
     3661 ctggcggccg agctcggatc gcgtggcatc cgtgtccatg cgatctcgcc cggcccactt
     3721 gcgacccgtg cagcctctgg aatcgcccat ttcgatgagc tgttgaacca ggcggcgaag
     3781 cgggctccgg cgggacggct ggtaaccatt gaggacgtcg gcgtcgcgtg cgccgggctc
     3841 gccaccgagg gtgcgcggct catcaccggc gatacgctct atgtcgacgg tggtctgcat
     3901 gtcctcgggt agcagatctc acgcggattt gagcgggcgc aatgatactc gctgcagtcg
     3961 ccgccctcgg ctccgaagtg acaagcggga gcccgaatca gtagccaacg gaccggaagg
     4021 ctgaatatgc aattgggaac cgtcgacatc gccacgcttc tgctggtcac cgtcggtccg
     4081 ttgaaggcgc tgatcgtttt cggcaccttg accgctcgag cggatagcga ctttcggcgc
     4141 caggtggcga tccgcacggt ggccgtcgca acaatcgtta ccttgctctt cgtcctggcc
     4201 ggcgagttcc tgctgcagat cttccacatc actctgcccg ccctgaaaat cgcgggcggc
     4261 atcatccttc tgctctttgc aattcatatg gtgatgggcg aggcgggtaa gaaagaggac
     4321 atcaaaatgc cctcgccgga tatagccata ttcccactgg ccatgccgat gatggccacg
     4381 ccacaggggc tcgtggcgat cacggtcatc gtcgcgggcc gtcaatccta ttccgacgtt
     4441 ctcgtcgtgg tggtgatcct cctggcgatc atggtgttca atctcgtttg catgctctcg
     4501 gctgatcgca tcatctcggc catcggaccg gcggcgctgc aggtcatcgc gaaaattgcg
     4561 ggtctccttc tcaccgcact cgccgttcag ctcatgattt cggcattccg ggatctcggg
     4621 ctgatcgagc gagtcattgg agcgacggga cattgaccac agacgagatc caggcggaaa
     4681 tatcggaacg gaagcccgga cggcggtggc ttgcacctgt tctcgcggtt tgcgccgcta
     4741 tcttcgtcta caatctcatt gccgatcgcc tgaccccgta cacctcgaaa gcaaccgtgc
     4801 aggcctacct catcggcatc gcccccgagg tcgcggggac ggttatcgat gtcggtgtcg
     4861 tggacaacca gcgggtcaag gcgggcgatg tgctgtttcg catcgatccg cgccgttacg
     4921 aagccgcgct gcgagaggcg caggcggccg tcgaccgcgc cggtcagtcg atcggcgcca
     4981 atacggcggg cgtggcggcg gccgaggcgg cgctcaccga tgcccgagcc caatacgcca
     5041 acgcgcggga acaggccaca cgaatcttcg agctggtcaa gcgcggcatt tatgccaagg
     5101 cccgcggcga ccagatgcaa cgtttgctcg agggtgcgca ggccggtgtc cgccaggcgg
     5161 aagcgcagct ggagcaggcg cggcagcaac tcggtccgaa aggagagagc aatccggagc
     5221 tccaggctgc cctgtccgct ctcgagcagg ccaggctgaa cctcaacgat acggtcatgg
     5281 tctcgcccgc caacgggcgc gtgaccaatc tcaggctcat tggcggccag ttcgttgccg
     5341 ctggtcagcc ggccctgacc gtcatcgaca cccaggcgat ctggatctcg gcgaatttcc
     5401 gggaaaacaa tctcggcaac atgagccaag ggaacccggc ggaaatcgtg ctggacgtcc
     5461 gcccgggtga ggtttttccg ggcgtcgtgg agagcatcgg ctggggcgtc gacgtgggcg
     5521 aaacgacggc cgggtccggc ctgcccgtga tccggaaccc aagcggatgg cttcgagaag
     5581 cacagcgttt ccccgctcgc gtccgcttcg aaaccgacgc ctatccccgc ggtattcgat
     5641 acggcgcgca ggcgaacatc atcgtctaca ccactgacaa ccgggttatg aatggtctcg
     5701 catggctctg gatccgcgcc gtcgcttggc tttcctacct ctactgagcg gcaagagacg
     5761 gcagaggctg caccgcggct cgaggagcgg ccggttcccg cagagcggca tcaagccgct
     5821 catgccgaac ggacgcgcgg ccttggacta cgggtcggat ttgccgtaac ggccgggttc
     5881 ctcgtcagcg aactgctcga ttgggagctt tccttcctga ctcccgtatt ggccgtgcag
     5941 cttctcgccg cagcaccggc gatgccgagc ttccgacaag ggcttgcgat aatcgtggtg
     6001 ttcggcggca ccaccggcgc ggcgctcgtg gcgagcgaac ttttcgccaa tatgcccgtg
     6061 ctgttcactg ccctgctcgc gctggtcacg ctttgggcct tctacctaca ggctcgccgg
     6121 caacagcaac tgctgtcgat gttgcttctg gtctcgttca ccatcgtgcc cgtggtcgcc
     6181 atccgtgaac cgaccgcggc agcggtcgtt gccttctccg tattccgcag cggcatcacc
     6241 gtcgtcctcc tgatctggct cgcgttcacg ctcttccctt cggatccgat accggaaatg
     6301 cccgggaagc cgattcggct gcccttgcgc gcccgcgagc ccgcaatgcg cgaggcactc
     6361 gtggacaccg ccgtcatcat gccggtgctc gttgccgccc tgacgttcac cacgacggcc
     6421 gtcatcttta tcgttatgac gatctctgcc gtccttagcc agcgcgcctt cgcgcaggca
     6481 tcgcagaccg cgctcggtct gtttcttggc aatttactcg gcggtgcaac cgccatcgtc
     6541 gcctatcagc tgctgcgtgc ggtgccgtca ctgggttttt tcaccgcact cctgctgttt
     6601 attggtcttt cgtatggctt cgccatcgag cgcggaggca ctcggagcca tttgctggtt
     6661 gttgcgctgg tcactttcct gatcgttctt ggcgtgggaa tatcgccctt cctcgacgag
     6721 ccgaccgcga gcctttccac ccggatcgtc aatctcggat ttgcctgtgc ctatgcattc
     6781 cttgcccttg cattgattca ggcacttcgc cggcccgagc agccacgctg gcccgcagcg
     6841 tcaagcccgg atgcaccggg cgcggcatga ttgataggtc ggcggggcaa gccatgcccg
     6901 aagcggaggc aggcaggaga tgcagatggc aaaaagatac cgcccgaagg gggacgccgg
     6961 catcggcgca ctgacgcgcg ttctcttctc agcggtactt tccttctcga tttgcgcttc
     7021 cgcgcaagcc agtgaggacg ttcaagaact tattcggcac cagttcgaga tctatgaccc
     7081 ggcttatggg gctcatcggg aacagtatgg ggaacgcctg aaagcgatgt ccgaggcaat
     7141 cgccgaggcg caggccaatg gccgaaatct tcattgtacg cagcagatgt atctcgaagc
     7201 caagtggctg taccgctaca ccgcccactg ggaccgccta gcggacaaac tcacgcgaat
     7261 tgagcagagc ctagacgacc ctgaccagag ctttgctgcc gagcagtcgc ccgtggacgg
     7321 catgtggggc atctgctacg aagaatggtt catgcgcttg gccgccaccg tcgacgggct
     7381 ggagaatctc gccgcacgag gcgagcggcc ccggtatcaa ttgcgatccg ccggcaagtt
     7441 cgatacgggc aagaaactgg tgacgcggat gcaaggtctt ctcatatcgg acatcgccca
     7501 caccggggtc gacaatcgag gagagctctc gagcctcatc acctccattg cacaaggtgc
     7561 gttcaagccg ctgttgcgag acatgcttgt caaaagcgcc gatttgcgca tttcctccgg
     7621 gcttgactcg gtcacggagg catttcggtt cttcctcagc ggcgctcaag acccgacaac
     7681 cggctattgg ggggcttggt acattgtcga cgggaagatc cacaagacct ccgacctgag
     7741 catgacctac cacgtcattg catacactaa tggtcgagta gagcgctggc cgcagatcat
     7801 tgcaacgacc aaagccatcg aggccctgcc ctatccatat gggtggcggc acaatggcaa
     7861 gtacaacaat cacaaccttt acgatgtggc aaaaatctac aagttcgcat ggccgcatat
     7921 gtcgaaagca gaacatcttg agatcgccaa acagatcgac tcgatgattc attggtcgtt
     7981 agcaaataca ctcggaccgg atgaccagtt tctgcacgat ccaaccttct cggattctct
     8041 gacagacgaa tactacttcg gggtctcgtt cctggatgtg gtcggatact ggaagcaaga
     8101 tcggcggttc tggacgcctt cccttgtcga taaggaaggc gccgccctgt gttgccgtct
     8161 gaagcaccga atgacggaac tcgacttgga aggttgggca gcaaacggag cgatgaggaa
     8221 gctcgaaaga aattgtagcc agtgcccagc acccaatgct gaagaatgaa ggccattaca
     8281 gacgtggcaa gaagcgacca ctcctgaact cgagccactc tcaccaagac ggctttgcca
     8341 tcaaaatggc tccgtccttc ccgtccgatc tgcaccgcat ccggttcaag cccctggcgc
     8401 gggggtgacc ggaagtcgga tcgaggaagt cctcttccct tgcggggagt gggctctcag
     8461 gcgggcccgg cgccttgctg cgttcgctac ccggtcttgc ggcgcagcag ctcgatgagg
     8521 ccggagaact cgccgccgct gctctggatc acggagccga aatcggcccg ctgggtcacg
     8581 gccatgctga tgccttcgac gacgatgtcg accaccttga acgcgccgtc gcggccgcgc
     8641 agccgccaat cgacttggac gttctccgcg cctggccgga tcaccgtgct ccgcacgatg
     8701 gcgtagccct gttcctgccg cacaccttgg acttggagct gctcgccggc atagtcggcg
     8761 aagcgggcgg cataggactt cacgaggaac gcctcgaaca ggcggaggaa ctcctctcgc
     8821 tcggcctcgg aggcggtgcg ccagtagcgc cccagcacga accggccgat caccgggaga
     8881 tcgaagctcc ggtcgagcat gccgcggaaa cggcgctcgc gctcgctctc ggagagctcg
     8941 ggaccggtca gggcctcgac ggcttccatg cccaactgct cgatgaagcg cttcgggtcg
     9001 aggtcggcct gctcggcgcg ggagctcccc cgcgcgagca gcgcagcgaa gccggcaaga
     9061 ccgatagtga ctctgcgacg tgacagcatg gctcagttcg tgtccgtttc gaaatcgagt
     9121 attccctccg ggtcatcctc gaaggtcgcg tcggggaagg cattggactc gggccggcgc
     9181 gccgttgcgg atcccgtctg caccgggttc ggcggggcca cgtagtacca gccggcgacc
     9241 ggctgaagcc tcgaagagcg aagccctgca ccgctgccgt gcctatccct ccgcagggtc
     9301 ggacaaagtc gggccagcgg tccctatcat cgttccgcgg tccgcgccgt ctcccgttcg
     9361 tcgctcgccc gcgcccgtcg gtatcctcag tgatgccaca cgatgccctc atggtcggca
     9421 ctaaccagcg tccccttatc gcccgcgaca atggccggga tctcggccag cgcgccgatg
     9481 gcggcggtct tgccggtcgc ctgcgcgaag cggcaggcgg catcgacctt gggccccatg
     9541 gagcctgcgg ggaactggta ttctcgcatg gccgccggac tggcccggtg aatcgccttg
     9601 gcatccggct cgccccagtt cacgtagacg cccgaggcat ccgtcgccat gatgaagagg
     9661 tcggcgtcca attcgcgcgc caagagttcg ctcgctagat ccttgtcgat caccgcctcg
     9721 acgccgatca gccgtcggtc ggcgtcgcgc tcatacatcg tcggaatgcc gccgcctccc
     9781 gccgcgatga cgatggtccc cctatccaag agccagcgga tcgggcggat ctcgaagatc
     9841 cgcttcggtt cgggtgacgg cacgacccgc cgccatttgt ctccgtcggc tttgaagatc
     9901 cactcttttt ctttggcgat ccggtccgcg cctgccttgt cgtaaaccgg acccacgaac
     9961 ttggtcgggc tgcggaagcc gggatcgtcg ggatcgactt cgatcatggt gagcagcgtg
    10021 gcgaacggca cctcgaacgg cagcaagttg ccaagctcct gctcgatcat gtagccgatc
    10081 atgccctcgg tctccgcacc gagcacgtcg agcggatagg cttcgccggg cttgtaggat
    10141 gctccttgga gggcaagcag cccgacctgc ggtccgttgc cgtggctgat cacgagctga
    10201 tggtcgcgcg cgacaggcgc taaagcctcc gccgcgacct tcacgttgcg gcgctgagca
    10261 tcggcggtca tcggctcgcc gcgtttcaga agcgcattcc cgccaagagc gacaacgatg
    10321 cgcatggcgg tcagtccccc agtgtggcga ccagaatcgc cttgatcgta tgcagccggt
    10381 tctcggcttg ctcgaaagcg atgttccttt ccgattcgaa cacgtcgttg gtgacctcga
    10441 ggccattcct catgccgtag gcttctgcga tctgccggcc cactgtggtt tcggtatcgt
    10501 gaaaggccgg caggcaatgc atgaacttta cctgcgggtt ttcggtggct tccatgagct
    10561 ccgcattgac ctgatagggc ttcagcagtt cgatcctctc cttccaaacc tccttcggct
    10621 cacccatcga gacccagacg tcggtgtgga tgaagtcgac gccttgcacg gcagttttcg
    10681 gatcatcggt gacggtgagt ttggcgccgc acctgcgtgt cagatcctca gcaatccccc
    10741 tgtactcctc gctgggccac agcgtcttgg ggccgcagac gcgcacatcc atccccatta
    10801 gacagccggc gatcatcagc gaatggccca tgttcgagcg ggtgtcgccg acataggcgt
    10861 atttaatgtc gtggatgggc ttgtcgctgt attcgcgcat ggtcatgatg tcggcgagca
    10921 tctgcgtcgg gtgatattcg tcggtcaatc cgttgtagac cggcacgccg gcgtatttcg
    10981 cgagctgctc cacgccctcc tgcgaggccc cgcgatattc gatcgcgtcg tacatgcggc
    11041 ccagaacgcg cgcggtatcc ttgtaggatt ccttgtgtcc gatctgcgac ccgaccgggt
    11101 ccaaataggt gacatttgcg ccctgatcgt gacaggcgac ctcgaaggca cagcgcgtgc
    11161 gggtcgaggt tttctcaaaa atcaggcaga tttctttgtc ggcaagatgc cgttgttcgg
    11221 ttcgcgcgta tttcgcgcgc ttcaggtcgc gcgcgaggtc caggagatag cggaactccc
    11281 gctgggtgta gtcctgcacg gtcagaagcg agcgattgcg tagattgaag ctcacgggat
    11341 cgcctccttt tcggactgaa tgggtcaggt caataggcgg ggtcgcggga gacggggcag
    11401 gtcatgcaat gccctccgcc gcgcccgcgg cccagctcgg cgccgcgcac agtgatcacc
    11461 tcgatcccgg cctttctgag cagggtgttg gtgtgcacgt tgcggtcgta ggcgacgacg
    11521 acgccgggct ccagcgccac gacgttgttc ccgtcgtccc actgttcgcg ctgcgcgtcg
    11581 taagcgtcgc cgccggtctc gacggcccga agcgtgttca gtccgagcgc ctcctggacg
    11641 acctggagca tcggacgctc ctcgcggcgg acctcgatgc cgaccggttt gctgcccggg
    11701 tacacgctgt agcaggcgat ctggtccacg acttcgcgga agacggttac aacgtcgcgg
    11761 tcacagaagg tgaagaccgt gtccagatgc atggccgccc ggctcttcgg catcaggcag
    11821 gcgatgacgc gcgaggccgc ctcgttccgg aacagctcgc acgcaacccg catcaccgcc
    11881 tcgcggcttg tccgctctcc cataccgatt agcaccagcc ctttgccgac cggcatcaca
    11941 tcgccacctt cgagggtggc gtaggcgaaa tcttcgtccg aatcccccca ccagatccgg
    12001 aaatcagcct ttttgaacag cggatggaat ttgtagacgg cgcgctgaag cagcgtttcc
    12061 ggtttgcgtg cgggccagaa catggggttg caggtgaccc ctgcgtagat ccagcaggag
    12121 ggatcccgct ggaataatgt gttcggaatc ggcgggatga cgaagtcggt gcctccgaat
    12181 gcatcgccta acaatctctt cacgccgttt tccggaagat cgtcgacggc gatgccgccg
    12241 atcagatgct cggcgagcag cttggccggc atctcatcga gccaggggcg catcgcc
//
LOCUS       DASVDU010000082        20840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_817557, whole genome shotgun sequence.
ACCESSION   DASVDU010000082 DASVDU010000000
VERSION     DASVDU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20840
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817557"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF226_05270"
     CDS             complement(<1..248)
                     /locus_tag="VF226_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7073432.1"
                     /translation="MRKSKLTRRRFLTTTAAASAAVIAAPYVRTAHAAGKLSVGFWDH
                     WVPGANDATKKAVEEWAAKEKVEVQMDFIPSQGNKNLLT"
     gene            614..1828
                     /locus_tag="VF226_05275"
     CDS             614..1828
                     /locus_tag="VF226_05275"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX7073433.1"
                     /translation="MQKIKVTGAVVELDGDEMTRIIWKLIKEKLIYPYLDINLEYYDL
                     GIENRDRTNDQVTIDAAHAIKKHGVGVKCATITPDEARVKEFGLKEMWKSPNGTIRNI
                     LGGVIFREPIICKNVPRLVPGWTQPIIIGRHAFGDQYRATDFKFQGKGTLTIKFVGED
                     GTVIEKEVFKAPGSGVAMAMYNLDDSIRDFARASFNYGLARNYPVYLSTKNTILKVYD
                     GRFKDIFQEIYDAEFKAEFEKRGLTYEHRLIDDMVASALKWSGGYIWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGKETSTNSIASIFAW
                     TRGLAHRAKLDNNPELAKFAETLERVCVETVESGYMTKDLALLVGADQKWLSTTGFLD
                     KVDENLKKAMGA"
     gene            2426..2941
                     /locus_tag="VF226_05280"
     CDS             2426..2941
                     /locus_tag="VF226_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEX7073434.1"
                     /translation="MRRAPALRQRAARHALRKAPKNLALINRGLRHNPGQSKPAHVQH
                     DPKRKAGKSGWAHRHRPFYFKHGGHRWRRHYYSVFVGGLWYWYWYDVIADTDPAVVIY
                     SEADLPECDLESDDCVEPEPAPLVAPAILEGRATEEAMARCAATFKSFNPETGTYLTY
                     RGVVRPCPYLE"
     gene            complement(3210..5858)
                     /gene="pepN"
                     /locus_tag="VF226_05285"
     CDS             complement(3210..5858)
                     /gene="pepN"
                     /locus_tag="VF226_05285"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX7073435.1"
                     /translation="MKTDVPRPIRLKDYTPPDYLIDKVGLDFALDPTRTRVFSRLEMR
                     PNPAAKKKGQPLSLDGENLELGEVRLDGKVLEASAYEVTDRHLILPRVTRKPFTLEIE
                     TFINPEANKALQGLYRSRGIYCTQCEAQGFRRITYFLDRPDVLAVYTTRIEANAEEAP
                     VLLSNGNPVERGTLADGKHYAVWKDPHPKPSYLFALVGGNLSAVTSTFRTRSGRNVDL
                     GIYVEPGKESRCGWAMDSLKRAMRWDEERFGLEYDLDVFNIVAVSDFNMGAMENKGLN
                     IFNDRLILVSPETATDAVYEAVESVVAHEYFHNWTGNRITCRDWFQLCLKEGLTVYRD
                     EEFSADERDRTVQRIADVRQLKTHQFPEDAGPLAHPVRPDTYIEINNFYTATVYEKGA
                     EVIRMIETIVGREGFRAGMDLYFERHDGQAATVEQFVKCFEDANNVDLGQFMLWYKQA
                     GTPELICSLDYNETRKEAKLTVEQVLNPTPGQPTKRPMDIPLKLGLLGGNGQDIDLKL
                     ADGTPVKNGVVRVRKRKQTFRFVDVPSKPVPSLLRGFSAPVKLTVDLSDRDLEFLMAN
                     DSDLFNRWQAANTYAIRTLTTLVKTPTTSRRPAEGIAYAKALGATIRDETLQPAYRAE
                     MLRLPSEADMAREIAHNIDPDAIHRARKQLLRVVGETLGDALEDIYASYDSSKFSPDA
                     ASAGRRALRNAALTLLSARNSAADKARLVDHYWKATNMTDAAHALYLLAARGGADAKK
                     VLDDFYERWKGDHLVIDTWFAAQATSPLPGTLARVKALTKHPLFSLTTPNKVRALIGN
                     FASMNPVQFNRPDGAGYDFVAEQVLAIDKFNPQIAARMLAGFRSWRELEPGRRKLARK
                     ALREVAKTKGLSRDVTEIVSKMLEAA"
     gene            complement(5938..7059)
                     /locus_tag="VF226_05290"
     CDS             complement(5938..7059)
                     /locus_tag="VF226_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7073436.1"
                     /translation="MVKPLEGLLVVSLEQAVAAPMASRRLADAGARVIKLERPEGDFA
                     RGYDAVVNGLSSYFVWLNRGKESVVVDLARAEDRALFEAMLARADVFIQNLKPGALAR
                     LGYPAEELTKRFERLIACSISGYGDSGPYADRKSYDLLIQAESGLASVTGGPEAPARV
                     GVSVVDIAAGMNAYEAILEALIVRGRTGRGADIRVSMFDCMAEWMTVPLLQGEHGFPP
                     KRSGLAHVSISPYGVFQTADGVPILISIQSDREWAQLCRKVLERPELASDPRFATNVV
                     RVQNRPETDGIVAEWFAAHDHESAIARLAMADIAFARVNDVAGLGVHPHLRRVTVGTE
                     TGAVQMPAPGARFSGEAAQFGSVPALGEHTERARREFLG"
     gene            7122..7832
                     /locus_tag="VF226_05295"
     CDS             7122..7832
                     /locus_tag="VF226_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX7073437.1"
                     /translation="MDFDHVRERVQARGFVLRGGFHPVPGDAVPQLSDGRAAKTVVLL
                     GGVGGSFWPAFRASPEAEHEHEALDRWTRRVAGELAEELGAMPLFPFGGPPYLPFQRW
                     AKRAEPVHDSPLGLLIHPEFGLWHAYRAALAFADRLDLPVRPEAESPCASCLDKPCLT
                     ACPVSAFTGKSYDVPRCIAHIGRPEGGACIENACLARRACPVGRAYAYEPEQARFHMS
                     AFLRALRTQGRGPVPESA"
     gene            complement(7799..8722)
                     /gene="speB"
                     /locus_tag="VF226_05300"
     CDS             complement(7799..8722)
                     /gene="speB"
                     /locus_tag="VF226_05300"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7073438.1"
                     /translation="MSDNRLTELEYLPASEAFLEPDRADADRPNEARAVVIPFGLEAS
                     VSYGGGTAAGPAAILAASHQLELFDEELWREPYLDYGIATLRPPVVEQPLEAALDQLS
                     TIVGTVLEQGRFPFTIGGEHSLTPGAVRPFAATYPDLVVLQLDAHADLRDGYLGEHYS
                     HASAMRRVLDHPNVTLVSVGIRAISSGEVAYLEANRNRIYIHWAKDQARWDLEEIVAP
                     LRDRPVYVTFDIDALDGGIMPATGTPTPGGLQYLRSLEILKRAAEVSRIVGADLVELA
                     PIQGLHAADYTAAALAYKMMSYALSGTGPRP"
     gene            complement(8801..9976)
                     /locus_tag="VF226_05305"
     CDS             complement(8801..9976)
                     /locus_tag="VF226_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX7073439.1"
                     /translation="MERFPSAAALIAHSKPVRPVLAARPHAAGRAARWFLENFPGEVA
                     YAYKANSSVFLLGALFGAGIRQFDVASLPELEDAATIPGVTLHFMHPVKSRTAIRRAY
                     FEFGVKCFALDTEDELAKIVEETRGAKDLQLFVRVAVPAKNSRIPLERKFGISGQKAA
                     RLLMKTRQVADELGLTFHVGSQTTTPDAFITALEEVHKLIVKAGVVVDAIDVGGGFPS
                     RYTDSEPAPLSTYIDAIRTGFEKLPVSEGCRLICEPGRALVAEAESLIVQVDARKGNE
                     LYINDGGYGALFDSAHLGFVFPVRLVGRERAAGERLVPFSMWGPTCDSIDYMKGPFML
                     PESVGEGDYIEIGNIGAYGRAIASNFNGYGRYDEVILLDEPMYTMYGDVRRQVAQNA"
     gene            complement(10167..10862)
                     /locus_tag="VF226_05310"
     CDS             complement(10167..10862)
                     /locus_tag="VF226_05310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7073440.1"
                     /translation="MKSWVEYWNSDHPIYVSERHKRVHAAAVGQDILRQIHTSDAVVL
                     DHGCGEALYAEAVAAGCAKLFLCEAAPAVRQKLASRVAGIPATEVIDADAVAALEDAS
                     LDLVVANSLAQYLTSAQLIGLLDLWRRKLKPNGSLVIADVVPPNVSPITDAAALLRLG
                     WQGGFLGAALAGLVKTALSDYRRVRATLGFATYSEAEFLALLRANRFEGRRIRPNFGH
                     NQARMAFRATPAP"
     gene            complement(10924..11937)
                     /locus_tag="VF226_05315"
     CDS             complement(10924..11937)
                     /locus_tag="VF226_05315"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX7073441.1"
                     /translation="MSAAGRALITLACCLLLLPAAAVGQGSSSALQDWVSPLQRDHPL
                     AGVIWSAEKGQAITPDELLRALAESDVVLLGEVHDNPDHHRLHAWVIGELASRRQASG
                     ETGPAVVLEHIRADQQHVLDRALTSRRAMRARDLLEALEWERSGWPSAAMFVPLFEEL
                     LSRALPILGGNTAASTIREVARGGLEALPAEERSRLGLDAQLDPPLNAALTAEIERNH
                     CGLLPESAFSPMSLAQRYRDAHLADVVLSAKEAHGAAVLLAGNGHVRADRGVPWHLRR
                     RSPETRSVTVAFAEVESGGKDAAAYVPLDPSGAPAVDYVWLTPQAERPDPCERMRQRY
                     EKR"
     gene            complement(11940..13178)
                     /gene="carA"
                     /locus_tag="VF226_05320"
     CDS             complement(11940..13178)
                     /gene="carA"
                     /locus_tag="VF226_05320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX7073442.1"
                     /translation="MTPSTDQNGLGTRRHAPAPWTETPLTARLVLADGTVISGRGLGA
                     VGSAVGEVCFNTSMTGYQEILTDPSYAGQIITFTFPHIGNVGTNAEDSETSNLAMRSG
                     VRGCVLRADVTAPSNYRAVEHLDAWLKARGIIAITGVDTRALTARIRTGGTPNGVIVH
                     EPTGEFDIEKLKAEARAWPGLVGLDLVPEVTAGQSYTWDEMRWVWGKGYPRQLNPEFH
                     VVAVDYGLKRNILRSLASAGCKVTVVPATTTAEEILERKPDGIFLSNGPGDPAATGKY
                     AVPQIRRLVDSGLPVFGICLGHQMLGLAFGARTVKMHQGHHGANHPVKDFTTNKVEIT
                     SMNHGFAVDRETLPSELEETHVSLFDGSNCGLRLKDRPVFSVQYHPEASAGPQDSHYL
                     FTRFVNLIREQKGLPLQSER"
     gene            13372..13839
                     /locus_tag="VF226_05325"
     CDS             13372..13839
                     /locus_tag="VF226_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7073443.1"
                     /translation="MRDQLSQSIKDAMKSGDKRRLATLRLISAAIKDRDIAAQVDSSG
                     QPSGRDKISEAEELQLLQKMVKQRREAAETYRKAGRQDLADQELAEIAIIEEFLPRQL
                     NEEETRAAVEQVVRELDCAGLKDMGRAMAALKERYSGRMDFGKASGIVKELLK"
     gene            13978..15885
                     /gene="dnaG"
                     /locus_tag="VF226_05330"
     CDS             13978..15885
                     /gene="dnaG"
                     /locus_tag="VF226_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7073444.1"
                     /translation="MRFPPAFLDEIRARLPVSQVVSRKVALKRKGREYAGLSPFKTEK
                     TPSFFVNDQKGFYHCFASGEHGDIFTFLMKTEGLSFPEAVERLAAEAGVPMPRATDAD
                     NRREDQRARLLAVLEASLAFFQAELRGSAGLEARRYLERRGLSHETIARFRLGYAPPG
                     RAVLKDHLAGLGFTPEEMTLSGMVVAGEDIPVSYDRFRHRVMFPITDLRGRVIAFGGR
                     ALDPEAPAKYLNSPETQLFHKGAVLFNASQARAAAHDHDEIIVVEGYMDVIAMSQAGF
                     EQTVAPLGTALTEDQLRLLWRMAPEPILCFDGDAAGRRAAYRAIDVAIGHLQPGRSLR
                     FAFLPDGSDPDDLVRQQGPEAMRRVLEKARPLVEVLWEREWRSGQWSTPERRAFLEKR
                     ISTLLGRIGDATVKQHYGRALRAKLYEAWPVTAAGTSRVDRAQWSRQPYRSGSPPRFG
                     AQPRPGGRFANPQKPRPSDSLKRSGLVGPGHAPYREALLLRTLLNHPWLVEEHAEAIA
                     GIDFTLTALGRLRDAILSLKSNDISLDSSRLRTQLEAQSLQQAVDLVERAITHKCDRF
                     AEADAERADVEAGFRHALALHERQKGLKRALEAAERDFLEEGSEESLARICEVQRLIA
                     SLDVVDAPLDF"
     gene            16180..18153
                     /gene="rpoD"
                     /locus_tag="VF226_05335"
     CDS             16180..18153
                     /gene="rpoD"
                     /locus_tag="VF226_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX7073445.1"
                     /translation="MTQKTEEQTPPQVEMPERDSPVLDLSDQAVKRLIKSAKARGYVT
                     YDELNAVLPSEEVSSEQIEDVMAMFSDMGINVIESEESEESPDEDSLDDEEGRALTTQ
                     TLPARAESRPEPVERTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREAMIAGLCE
                     SPLTFQAIIIWRDELNEGKVLLRDIIDLEATYEGPDAKAAPVEAEEGDEEDEASRDGE
                     ANGAARGDMDDDDDYENAMSLAAMEAELKPKVLETFDRIANAYKKLRKQQDRNLEMQV
                     AGEQGSRQQQKAYAKLREEIIADVKSLQLNNNRIESLVEQLYAINKRLVGLEGQLMRL
                     ADSYGVARQDFIDEYYGNELDQTWLDRIAKSGKKGWAKFIENERARIEDIRGEIHNLA
                     TETGLEIPEFRRIVRSVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQ
                     EGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKIV
                     RTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKEPISLETPIGDEEDSHLGDF
                     IEDRNAVLPIDAAIQSNLRETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQF
                     SVTRERIRQIEAKALRKLKHPSRSRKLRSFLDN"
     gene            complement(18406..19005)
                     /locus_tag="VF226_05340"
     CDS             complement(18406..19005)
                     /locus_tag="VF226_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073446.1"
                     /translation="MNNRSIMRLLICGAVSITAAVLAQPSVALAQGKSQPPQQATQPA
                     LPDGYKMDMLIRTTLIALSQANLTGNYTVLRDLGSPSFQSVNSAARLTEAFADLRHRQ
                     LDFAPILFFSPKLVREPALDEAGRLRLRGFIETRPEQINFDMLFENVGGDWRLFGLAV
                     QMQPAPASTAAAGQKEAPAAKQPAAKQKAATKTSAPAKK"
     gene            complement(19185..19856)
                     /locus_tag="VF226_05345"
     CDS             complement(19185..19856)
                     /locus_tag="VF226_05345"
                     /inference="COORDINATES: protein motif:HMM:NF017472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073447.1"
                     /translation="MEINHTIALGTDSLASSDNAIAIGRDSAASGGNSLAVGRGSTAS
                     GSNSMALGRNTSATGNGSVALGTDNTGQGAQATLPNEFALGTARHTYTAAGITSLESR
                     QRQQGPLEVVTSDANGHLATDGGLLFDELSRIGGGVAIAMALENPDLVGNEKFGLAGN
                     VAFWEDNVALGFTAMGVLGHNFLGAGERWALSGGVGFTVKEESYGRRGSKSSVGGRAG
                     LQVSW"
BASE COUNT         3839 a         6705 c         6610 g         3686 t
ORIGIN      
        1 gtgagcaggt ttttgttgcc ttgcgaagga atgaagtcca tctggacctc caccttctcc
       61 ttcgccgccc actcctcgac cgccttcttg gtcgcgtcat tcgcgcccgg gacccagtgg
      121 tcccagaagc cgaccgaaag cttccccgcc gcatgggccg tgcggacgta aggggcagcg
      181 atgaccgccg cagatgcagc tgcggtcgtc gtcaggaagc gacggcgggt aagcttagac
      241 ttccgcatgg ggtttcctcc attcatgccg acacgtatcg actttccatg catgcaaaac
      301 ggcagagcgt gctccgcaca cctggttgtt gccggccgat cttctcgccg gatgccgctc
      361 ttcacggctg cctgaaggta actgacggat cgtccgcaag gcaacagttg gtttgcgctg
      421 gatccgaccg ggtcccagtt ttcgcacaga gcggtaactt gtgtggccga gccgccctcg
      481 gcgggacgct gcgtctcatt atcccgctac ttggcttccc gcgccgatcc gtctatcacc
      541 ccgggcgagg cgtgccggcg gccggccggt gccgccagga ctctagaggc ccgcgcgatc
      601 tggtgagaaa gctatgcaga aaatcaaggt gaccggcgct gtcgtcgagc tcgatggcga
      661 tgagatgact cgcatcatct ggaagctcat caaggagaag ctgatctacc cctaccttga
      721 tatcaacctc gagtactacg acctcgggat cgagaaccgg gaccgcacca acgaccaggt
      781 cacgatcgac gcagcccacg ccatcaagaa gcatggcgtg ggcgtgaagt gcgcgaccat
      841 cacgccagac gaggcgcggg tgaaggaatt cggcctcaag gagatgtgga agtctcccaa
      901 cggcaccatc cgcaacatcc tgggcggcgt catcttccgt gagccaatta tctgcaagaa
      961 cgtcccgcgc ctggtgccgg gctggacgca gccgatcatc atcggccgcc acgccttcgg
     1021 cgatcagtac cgcgccacgg atttcaagtt ccagggcaag ggcacgctga ccatcaagtt
     1081 cgtcggcgag gacggcaccg tcatcgagaa ggaggttttc aaggcccccg gcagcggcgt
     1141 cgccatggcc atgtacaatc tcgacgattc gatccgcgat ttcgcacgcg cttcgttcaa
     1201 ttatggcctt gctcgcaact acccggtcta cctctcgacc aagaacacga tcctcaaggt
     1261 ctatgacgga cgcttcaagg acatcttcca ggagatctat gacgccgagt tcaaagcgga
     1321 attcgaaaag cgcggcctca cctacgagca ccgtctcatc gacgacatgg tggcttcggc
     1381 gctgaagtgg tccggcggct acatctgggc ctgcaagaac tacgacggcg acgtgcagtc
     1441 cgacacggtg gcccagggct tcggctcgct cggactcatg accagcgtgc tgatgacgcc
     1501 ggacggcaag acggtcgagg ccgaggccgc ccacggcacc gtcacccggc actaccgcga
     1561 gcaccagaag ggcaaggaga cctcgaccaa ctcgatcgcc tccatcttcg cctggacgcg
     1621 gggcctcgcc catcgcgcca agctcgacaa caaccccgag ctggcgaagt tcgccgagac
     1681 tctggagcgg gtttgcgtcg agaccgtcga gtccggctac atgaccaagg atctcgccct
     1741 tctcgtcggc gccgaccaga agtggctctc gacaaccggc ttcctcgaca aggtcgacga
     1801 gaacctcaag aaggccatgg gggcctgaag ccgcgttcct atggttgctc cgccgcgccg
     1861 tcctgttgtt ggcgcggcgg ctcccttgac gagaggggga cggccccgtc ctgcacctcg
     1921 cccgaccact cttagcagcg atccgatggt tgggcgttgc cccgctcccg ctaaaatggc
     1981 gatctacttt attgacgtcc gtcaatgaaa gggtgcttgg ctgctgattt gttgctgcac
     2041 tgaaacatgc agtcgtcgcg tgggggccaa catgattgta tgcagacgca tcgtgcgcct
     2101 cgcggcgagt gtcgggctta tttgcgtttt gggtctagcg tcctccgtta cggagggctt
     2161 ggcgcagaga tccggcggct tccgcaaggg gccggtcgcg agagatggcg ggtcgatcgg
     2221 caggagcctt tcatcacggc ccggaagatt tgctgtgccg cgcagcagca gggcggcggc
     2281 cacgccacgg gttgccagat ccctgcaacg ggcacggccc tccaggagag ccgcgaccac
     2341 ctctcgcagc cgcattgtga tgcccagacg gatcgggcgt agtgcgacgc gcattgcaag
     2401 gtcgcgcgca gcacagagta cgagattgcg tcgtgcgcct gccttacggc agcgcgctgc
     2461 acgtcatgcg ctgcgaaagg cgcccaagaa cctggctttg atcaaccgcg gactacgcca
     2521 caacccgggt cagagcaagc cggcccacgt gcagcacgac ccgaaacgca aagcgggcaa
     2581 gtcaggatgg gcgcatcggc accggccctt ctatttcaag cacggcggcc atcgctggcg
     2641 tcgccactac tacagcgttt tcgtcggagg gctctggtac tggtattggt acgacgtcat
     2701 cgcagacact gatcctgccg tcgtcatcta cagcgaagcg gacctgcccg agtgcgatct
     2761 cgagagcgac gattgcgtcg agcctgagcc cgcgcctttg gtggctcctg ccattctcga
     2821 agggcgggcc actgaagaag ccatggcgcg gtgcgccgcc acgttcaagt cgttcaatcc
     2881 cgagacgggt acgtatctga cctaccgggg cgttgtccgc ccctgccctt atctcgagtg
     2941 acggcaaagg gcttccgctt caggccaccg gctggagcat ttcctggtgg cggataggct
     3001 cacggaaaat gctccagttt tcgcttgccc gagcatcttc acccattcgg gcgagtcctc
     3061 ccgagtagtg ttccgtcacc aaagcctgac ccctcctgcg gcatggcccc gccgtcatcc
     3121 acacaggctt ccgccattaa gcatcgaggt gtcgtggatt cccgcctccg cgggaatgac
     3181 gtgtggtgaa cgatagcgac tgcaccttat caagctgcct ccagcatttt cgacacgatc
     3241 tcggtcacgt cgcgcgatag ccctttcgtt ttcgccacct cccgcagggc cttccgcgcc
     3301 agcttgcgac gccccggctc gagctcccgc cagctccgga agccggccag catccgggcc
     3361 gcgatttgcg gattgaactt gtcgatcgcc agcacctgct cggcgacgaa gtcgtagccc
     3421 gcaccgtccg gccggttgaa ctgcaccggg ttcatggatg cgaaattgcc gatcagtgcg
     3481 cgaaccttgt tcggtgtcgt cagcgagaag agcggatgtt tcgtgagcgc cttgacgcgc
     3541 gccagcgtcc cggggagcgg ggaggtcgct tgcgccgcga accacgtgtc gatgacaagg
     3601 tgatcgccct tccagcgctc gtagaaatcg tcgagcactt ttttggcgtc cgctccgccc
     3661 cgtgctgcca gcagatagag cgcatgggcg gcgtccgtca tgttggtcgc cttccaataa
     3721 tggtccacaa ggcgtgcttt gtcggctgcg gaattgcggg cgctgagcag cgtcagcgca
     3781 gcattgcgca gggcacgtcg gccggcgctg gctgcatcgg gtgagaattt gctggaatcg
     3841 tacgatgcgt aaatgtcctc gagcgcgtct cccagtgttt cgccgaccac ccgcaacagc
     3901 tgctttctcg cgcggtgaat ggcatccgga tcgatattgt gcgcgatctc gcgggccata
     3961 tccgcttcgc tcggcagacg gagcatttcg gcgcgatagg cgggttggag cgtctcatcc
     4021 ctgatggtgg cgccgagcgc cttggcatag gcgatcccct ctgccggccg cctgctcgtc
     4081 gtcggcgttt tcaccagcgt ggtcagcgtc cggatcgcgt aggtgttggc ggcctgccag
     4141 cgattgaaca gatcgctgtc gttggccatc aggaactcga gatcgcgatc cgacagatca
     4201 acggtcagct tcaccggggc cgagaagccg cgcagcaggg acggcacggg tttcgagggc
     4261 acgtcgacga accggaaggt ctgctttcgt ttcctgacac gcacgacgcc gttcttgacc
     4321 ggcgtgccgt ccgccagctt gagatcgatg tcctgtccgt ttccgcccag aagtccgagc
     4381 ttcagcggta tgtccatggg cctcttggtc ggctggccgg gcgtcggatt gaggacctgc
     4441 tcgacggtaa gcttcgcttc cttgcgcgtt tcgttgtaat cgagcgagca gatgagctcc
     4501 ggcgtgccgg cctgcttgta ccagagcata aactggccga gatcgacgtt attggcatcc
     4561 tcgaagcact tgacgaattg ctcgaccgtc gcggcctgac catcgtggcg ctcgaagtag
     4621 agatccattc cggcgcggaa gccctcccgg ccgacgatgg tctcgatcat gcggatcacc
     4681 tccgcgcctt tctcgtagac cgtcgccgtg tagaagttgt tgatctcgat gtaggtgtcg
     4741 ggccgcacag gatgcgccag cggccccgca tcctccggga actgatgcgt cttgagctgg
     4801 cgcacatcgg cgatgcgctg gacggtgcgg tcgcgttcgt ctgccgaaaa ctcctcgtcg
     4861 cgataaaccg tcagcccttc tttcaggcag agctggaacc agtcgcggca ggtgatgcgg
     4921 ttgccggtcc agttgtggaa gtactcgtgc gcgacgacgg actcgacggc ttcgtagacg
     4981 gcgtccgtgg ccgtctccgg cgagacgaga atgaggcggt cgttgaagat gttgagcccc
     5041 ttgttctcca tcgcgcccat gttgaagtcg gagacggcga cgatgttgaa cacgtcgaga
     5101 tcgtattcga gcccgaaccg ctcctcgtcc cagcgcatcg cccgcttgag cgaatccatg
     5161 gcccagccgc agcggctctc cttgccgggc tcgacgtaga tcccgaggtc gacattgcgg
     5221 cccgaccgcg tacggaaggt cgatgtgacg gccgagagat ttccgccgac cagagcgaac
     5281 agatacgagg gcttcggatg cgggtccttc cagaccgcat agtgcttgcc gtctgccagc
     5341 gtcccccgct cgaccggatt gccgttggag agcagcaccg gtgcctcctc ggcatttgcc
     5401 tcgatgcgcg tcgtgtagac ggccagcacg tccggcctgt cgaggaaata cgtgatccgg
     5461 cgaaatcctt gcgcctcgca ctgcgtgcaa tagatgccgc gtgagcgata gagtccctgc
     5521 agcgccttgt tggcctcggg gttgatgaag gtctcgatct ccagcgtgaa cggcttgcgc
     5581 gtcacgcgtg gcaggattag gtgacgatca gtgacctcgt acgcgctagc ctcgagcact
     5641 ttgccgtcga gcctgacctc tcccagttcc aggttctcgc catcgaggct gagcggctgg
     5701 cccttctttt tcgcggccgg gttcgggcgc atctcgagcc gcgagaagac gcgcgttcgc
     5761 gtcgggtcga gcgcgaaatc gagcccgacc ttgtcgatca ggtagtcggg cggggtgtag
     5821 tctttcagtc ggatcgggcg aggtacgtcg gttttcatga tccttccaat tgctggggag
     5881 acgaggtgca gccgacagca tccggatgtt ggccggatct ggcaagtctt gttgggtcta
     5941 acccagaaac tcgcggcgag ccctttcggt atgctcgcca agcgctggta ccgacccgaa
     6001 ttgcgccgcc tctcccgaaa accgcgcgcc aggagcgggc atctgcactg cccctgtttc
     6061 ggtgccgacg gttacccgac gcaggtgagg atggacacca aggccggcca cgtcattgac
     6121 ccgcgcgaag gcgatgtccg ccatcgcgag ccgcgcgatg gcagattcgt gatcgtgggc
     6181 cgcgaaccat tctgcgacaa ttccgtcggt ctcaggccta ttctgcaccc ggacgacatt
     6241 ggtcgcgaaa cgcggatcgg atgccagctc aggacgctcc agcaccttgc ggcagagctg
     6301 tgcccattcc cgatcgcttt ggatgctgat gaggatcggc accccgtccg cggtctgaaa
     6361 cacgccgtag ggcgagatgg agacgtgcgc cagccccgac cgcttcggcg ggaagccatg
     6421 ctcgccctgt agcagcggca cggtcatcca ttcggccatg cagtcgaaca tcgaaacgcg
     6481 gatgtccgcg ccgcgccctg tccgcccccg tacgataaga gcttcgagaa tggcctcgta
     6541 agcgttcatg ccggcggcaa tatcgacgac ggatacgccc acgcgcgccg gcgcttcggg
     6601 tccgcccgtt acggatgcca gtccggactc ggcctggatg agcaggtcat aggacttgcg
     6661 atctgcatat ggcccgctat cgccgtaccc gctgatcgag cacgcaatca gccgttcgaa
     6721 ccgcttggtc agctcttcgg ccgggtagcc gaggcgcgcc agcgcgcctg gcttcagatt
     6781 ctgaatgaag acgtcagccc gagccagcat ggcctcgaac agggcccggt cctctgcacg
     6841 cgcgagatcg acgacgacgg attccttgcc gcgattgagc cagacgaaat aagaggacag
     6901 gccgttgacc acggcatcgt agccgcgcgc aaaatcaccc tcgggacgct cgagcttgat
     6961 gacccgggcg ccggcatccg ccagccgccg gctcgccatg ggcgccgcca ccgcctgttc
     7021 cagcgagacg accagcagtc cttccaacgg cttgaccaac ggattttcct ccggatgctg
     7081 gctgagggcc gcgcaggtgg gcgcggggta aagcgatgcc gatggatttt gatcatgtcc
     7141 gggagcgtgt gcaagcgcgc ggcttcgtgc tgcgcggcgg ctttcatccc gtgcccggtg
     7201 atgctgttcc tcagctttcc gacggacgtg cagcgaaaac cgtcgtactg ctgggaggcg
     7261 tcggcggcag tttctggcct gccttccgcg catcacctga agctgagcac gagcacgagg
     7321 ccctcgatcg ctggacgcgg cgcgtcgccg gggagctggc ggaagagctg ggcgccatgc
     7381 ctctctttcc gttcggcggg ccgccgtact tgcccttcca gcgctgggcc aagcgcgcag
     7441 aaccggtcca cgactcgccg ctcgggctgc tgatccatcc ggagttcggc ctctggcatg
     7501 cctatcgcgc ggcgctggcg tttgctgatc ggctggactt gccggttcgg cccgaggcgg
     7561 aaagcccgtg cgcgagctgt ttggacaagc cgtgcctgac ggcctgtcct gtcagcgcat
     7621 tcaccggaaa gtcttacgac gtcccgcgct gcatcgccca catcggccga cccgaaggcg
     7681 gtgcgtgcat cgagaatgcc tgcctcgcgc ggcgcgcctg ccctgtcggc cgcgcctatg
     7741 cctatgagcc cgagcaggcg cgtttccata tgagcgcctt cctgcgcgca ctccggactc
     7801 agggccgcgg tccggtgccg gaaagcgcgt agctcatcat cttgtaggcg agcgcggcag
     7861 ccgtgtagtc ggcggcgtgc aggccctgga tgggcgcaag ctccaccaga tcggcgccga
     7921 cgatgcggct cacttcagcc gcgcgcttca ggatttcgag cgaccggagg tattgcaggc
     7981 cgcctggcgt cggcgtgcct gtggccggca tgatgccgcc gtccagcgcg tcgatgtcga
     8041 acgtcacata gacgggccga tcgcggagcg gggccacgat ctcctcgaga tcccagcgcg
     8101 cctgatcctt cgcccagtgg atgtagatcc ggtttcggtt cgcttcgaga taggcgactt
     8161 cccccgatga aatcgcccgg atgccgacgg aaacgagcgt gacatttgga tggtcgagca
     8221 cgcggcgcat ggcggaggca tgggagtaat gctcgccgag atagccgtcg cgcagatccg
     8281 catgggcatc gagttgcagc accacaagat cgggataggt tgccgcgaac gggcggaccg
     8341 cgccgggggt gagcgaatgc tccccgccga tggtgaaagg aaaccggccc tgctccagga
     8401 cagtccccac gattgttgac aactgatcga gggccgcctc gagcggctgt tcgaccacag
     8461 gaggtcgaag cgtggcaatg ccgtagtcga gatatggctc gcgccacaac tcctcatcga
     8521 acagctccag ctgatgactg gccgcgagaa tggccgccgg ccccgccgcc gtcccgccgc
     8581 catagctcac cgaagcttcg aggccgaatg gaatgacgac cgccctcgcc tcgttcggcc
     8641 ggtcggcgtc cgcgcggtcg ggctcgagaa acgcctcact ggcgggcagg tactcgagtt
     8701 ccgtcaggcg attgtcactc atccgaacga cgttccgtca aaggaaaccc cctctcaccc
     8761 aatggtggag agggggtttg ttaccattca gcttgtgcgt tcaagcgttc tgcgcgacct
     8821 gccggcggac atcgccgtac atcgtgtaca tgggctcgtc cagcaggatg acctcgtcgt
     8881 accggccata gccgttgaag ttgctggcga tcgcccgccc gtaggcgccg atgttgccga
     8941 tctcgatgta gtccccctcg ccaaccgact ccggcagcat gaagggcccc ttcatgtagt
     9001 cgatcgagtc gcaggtcggc ccccacatcg agaacggcac gaggcgctcg cccgctgccc
     9061 gctcgcgccc gaccaggcgc accgggaaaa cgaagcccag gtgggcagag tcgaacagcg
     9121 cgccatagcc gccgtcgttg atgtagagct cgttgccctt gcgcgcgtcg acctggacga
     9181 tcaggctctc cgcttccgcg acgagcgccc ggcccggctc gcagatcagt cggcagccct
     9241 cgctgacggg cagcttctcg aagccggtgc ggatcgcgtc gatgtacgta gacagcggcg
     9301 ccggctcgct gtcggtgtaa cgagacggga agccaccgcc gacatcgatc gcatcgacca
     9361 ccacgccggc cttgacgatc agcttgtgca cctcctccag cgccgtgatg aacgcatccg
     9421 gcgtggtcgt ctgcgagccg acgtggaagg tcagcccaag ctcgtccgcg acctgccgcg
     9481 tcttcatcag cagccgcgca gccttctgtc cggagatgcc gaacttgcgc tccagcggga
     9541 tacggctgtt ctttgcgggc acggcgacgc gtacgaagag ttgcaggtcc ttggccccgc
     9601 gggtctcctc gacgatcttg gcgagctcat cctccgtgtc gagcgcaaag cacttcacgc
     9661 cgaactcgaa gtaggcgcgc cggatcgccg tccgcgactt cacgggatgc atgaagtgca
     9721 gcgtgacgcc tgggatagtc gccgcgtcct cgagctcagg cagagaggcg acatcgaact
     9781 gccgaatgcc cgcgccgaac agagccccga gcaggaacac ggagctgttg gccttgtagg
     9841 cgtatgcgac ctcaccggga aagttctcga ggaaccaccg tgcggcccgg cccgcggcat
     9901 gcggacgggc ggcaagcacc ggacgaacgg gcttggagtg ggcgatcagc gccgccgcag
     9961 acggaaatct ttccatccca tgggacctcc accagtcggg aaatggacca gttggcgcct
    10021 tgctgaacca gcagagatcg ccgctacaaa atttcgaatg agacgccgag ataaggtcgc
    10081 ttgcccggcg tgtaaagagt aatgtagaga cccggcgcgc attttttgcg ggctcgtccg
    10141 aaaggccgca gaggcaaagt cgccgatcag ggtgcgggcg tggcacggaa ggccattcgg
    10201 gcttgattgt gcccgaaatt cgggcggatg cgacggcctt cgaaacggtt ggcccgtagc
    10261 agcgcaagga attcggcttc cgagtatgtc gcaaatccga gcgtcgcacg cacacggcgg
    10321 taatcggaaa gggcagtttt gacaagcccg gccaacgcgg cgcccagaaa cccgccctgc
    10381 cagccgagtc gcaacagggc cgcggcatcc gtgatgggac tcacattcgg gggcacgaca
    10441 tcggctatga cgaggctgcc gttcggcttc agcttgcgcc gccagagatc gagaagcccg
    10501 atcagctgcg cagacgtcag gtattgagcc agcgaattgg cgacgacgag gtccagcgat
    10561 gcatcctcga gcgcggcgac ggcgtcggca tcgatcacct cggtcgccgg gattccggca
    10621 acccgggacg ccagcttctg cctgacggcg ggggcggcct cgcaaagaaa caacttggca
    10681 cagccggcgg caacggcctc cgcgtaaagg gcctctccgc agccgtgatc gaggacaaca
    10741 gcgtcggacg tatggatctg gcgcaggata tcctgaccga cagcggctgc gtggacccgc
    10801 ttatgcctct cgctgacgta gatcggatga tcgctgttcc agtactcgac ccagcttttc
    10861 atggccggcc tccgacagat ggcagccagg atggtaacac cggcgcgccg gtgcgcatga
    10921 cgctcatcgt ttctcgtacc gctgacgcat gcgctcgcaa gggtcgggcc gctcggcctg
    10981 cggcgtcagc cagacgtaat cgacagccgg ggctcctgaa ggatcgagag gcacgtaggc
    11041 ggcggcatct ttcccaccgc tctcgacctc ggcgaaagca acggtgaccg accgggtttc
    11101 cggcgaccgc cggcgcaggt gccagggcac gccccggtcg gcgcgcacgt ggccgttgcc
    11161 ggcgagcagc acagcggcgc catgcgcctc cttcgcgctc aggacgacat cagcgagatg
    11221 cgcgtcgcgg taccgctggg cgagggacat gggcgaaaaa gcgctctcag gcagcagtcc
    11281 gcagtgattc ctttcgatct ctgcggtgag ggccgcgttc agcggcgggt cgagttgcgc
    11341 atcgagccca aggcgggacc gctcctcggc cggcagagcc tcgagccctc ctcgcgccac
    11401 ctcgcgtatg gtgctagcgg ccgtgttgcc gcccaggatg ggcagcgccc gcgacagcag
    11461 ctcctcgaag agcggcacga acatcgcagc agatggccag ccactccgct cccattcgag
    11521 cgcttcgagc agatcccggg cgcgcattgc tcgtcgcgag gtcaaggcgc gatcgaggac
    11581 gtgctgctgg tccgcacgga tgtgctcgag gacgaccgcg gggccagtct cgccagaggc
    11641 ctggcggcgg ctcgccagct cgccgatgac ccatgcatgc agccgatgat ggtcggggtt
    11701 gtcgtggacc tcgccgagca ggacgacatc gctctcggcg agggcccgga gcaactcatc
    11761 cggcgtaatc gcctggccct tctccgccga ccagatgacg cctgccaacg ggtgatcgcg
    11821 ctgaagcgga gagacccaat cttgcagcgc cgagctggag ccttgtccga cagccgctgc
    11881 tggcaggagc agcaagcagc aggccagggt gatcagagcc cggccggccg cggacatatt
    11941 tagcgctcgc tttgcagcgg cagacccttc tgctcgcgga tcaggttcac gaacctggtg
    12001 aagagataat ggctgtcctg cggaccggcc gaagcctcgg gatggtactg gaccgagaac
    12061 acgggccgat ccttcagtct caagccgcaa ttgctgccgt cgaatagcga aacgtgcgtc
    12121 tcctcgagct cgctcggcaa cgtctcgcga tccaccgcaa acccgtggtt catcgaggtg
    12181 atctcgacct tgttggtcgt gaagtccttt actgggtggt tcgcgccatg atggccctgg
    12241 tgcatcttta ccgtgcgcgc accgaatgcg aggccgagca tctggtgacc gagacatatg
    12301 ccgaaaaccg ggagcccgct atccaccagc cgcctgatct gcggcacggc atacttgcct
    12361 gttgcggccg gatcgccggg gccgttcgac aggaagatcc cgtccggctt ccgctccagg
    12421 atttcctcgg cggtcgtcgt agccggcacg accgtcacct tgcagccggc ggacgccagc
    12481 gagcgcagga tgttgcgctt gaggccatag tcgaccgcaa ccacgtggaa ttcggggttg
    12541 agctggcgcg gatagccctt gccccagacc cagcgcatct cgtcccacgt gtagctctgc
    12601 cccgccgtaa cttccggcac cagatccaga ccgacgagtc ccggccaggc ccgcgcctcg
    12661 gctttcagct tctcgatgtc gaactcgccg gtcggctcat gcacgatgac accgttcgga
    12721 gtcccaccgg tacggatgcg ggccgtcagc gcccgcgtgt ccaccccggt gatggcgatg
    12781 atgccgcgcg ctttgagcca ggcgtcgagg tgctccaccg cccggtaatt cgagggtgcg
    12841 gtgacgtcgg cgcgcaggac gcaaccgcgg acgccggacc gcatggcgag gttcgaggtc
    12901 tcgctatcct cggcattggt gccgacattg ccaatgtggg gaaacgtgaa agtgatgatc
    12961 tgcccggcgt agctcggatc ggtcaggatc tcctgatagc ccgtcatgga ggtgttgaag
    13021 cacacttcgc cgactgcgga acccaccgcg ccaagtccgc gtccgctgat caccgtgcca
    13081 tcggccagca caaggcgggc ggtcagaggt gtttcggtcc aaggcgcggg cgcgtggcgt
    13141 ctggtgccga ggccattctg gtcggtcgag ggcgtcatgg gctgctccgg gagctacggg
    13201 gtgccaagcc gacggtccga cgggcggccc gctacgttgc accgcaatat gaatccgtga
    13261 ccggcaacct atttgaggtc cctggcagcg tcaatcaatg gctcggtaag gcttgcgaca
    13321 accaccggcc gggcgtacat gacgcgccaa ttcgaaggcg aaggagacgc catgcgcgac
    13381 cagctcagcc aatccatcaa ggacgccatg aaatcaggcg acaagcgccg gctggcaacc
    13441 ttgcgcctca tttcggcagc gatcaaggac cgcgatatcg cagcccaggt ggacagctcc
    13501 gggcagccca gcggccgcga caaaatctcc gaagcggagg agcttcagct tcttcaaaag
    13561 atggtgaagc agcggcggga agcggccgaa acctaccgga aggccggccg ccaggatctc
    13621 gccgatcagg agttggccga gatcgccatc atcgaagagt tcctgcccag gcagctcaac
    13681 gaagaggaaa cccgagccgc cgtcgagcaa gtcgtgcgcg agctcgattg cgccggcctc
    13741 aaggacatgg gccgcgccat ggcagctctg aaggagcgct acagcggacg catggacttc
    13801 ggcaaggcca gcggcatcgt caaggagctg ctgaagtagc agccccgctg cggacaccgc
    13861 aggcctgcat gcctgtggat agcgggggcg aatgccacgg caagggcttg gtgcagcggc
    13921 attatccaac cgaatcgctt aacaggtgtg catcgtaatg ctcgacgtcg ggttccgatg
    13981 cgcttcccgc ccgccttcct tgatgaaatt cgcgcccggc tgccggtcag ccaggtcgtg
    14041 tcccgcaagg tggcgctcaa gcggaagggt cgggagtacg cgggcctctc gccgttcaag
    14101 accgagaaga cgccctcctt tttcgtgaac gaccaaaagg gcttctatca ctgctttgcg
    14161 tccggcgagc acggtgacat cttcacgttc ctgatgaaga ccgaggggct gtcgttcccg
    14221 gaagccgtcg aacggctggc ggcagaggcc ggtgtcccaa tgccccgcgc aacggatgcg
    14281 gataatcgcc gggaggatca gcgggcgcga ttgctcgccg tgctcgaggc atcgctggcg
    14341 tttttccaag cggagctccg cgggagcgct ggactggagg cgcgccggta cctggagcgg
    14401 cgtggcctgt cgcacgagac catcgcgcgg ttccggctcg ggtatgcccc gccaggccgt
    14461 gccgttctca aggatcacct cgctggcctg gggttcacgc ccgaggaaat gacgttgtcc
    14521 ggaatggtgg tcgccggcga ggacatcccc gtctcgtacg accggtttcg gcaccgggtg
    14581 atgttcccca tcaccgacct caggggacgg gtcatcgcct tcggcggccg cgccctcgac
    14641 cccgaggctc cggccaagta cctgaactcc cccgagacgc agcttttcca caagggcgcc
    14701 gtcctcttca acgcgagcca ggcccgtgcg gcggctcacg accacgacga aatcatcgtg
    14761 gtggaaggct acatggacgt catcgccatg agccaggcgg ggttcgagca gacggtcgcc
    14821 ccgctcggca ccgcgctcac cgaagatcaa ctccgcctgc tctggcgcat ggccccggaa
    14881 cccatcctct gcttcgacgg tgatgccgcc ggccggcggg cggcctaccg ggcaatcgac
    14941 gttgcgatcg ggcatctgca gccgggccgc agcctgcggt tcgccttcct gcctgacggg
    15001 tccgaccctg acgacctcgt gcggcagcag ggccccgagg caatgcggcg cgtgctcgaa
    15061 aaggcaaggc cactcgttga agtgctatgg gagcgcgagt ggaggtcggg ccagtggtcc
    15121 actcccgagc gccgggcctt tctggaaaag cggatctcga ccttgctcgg gcggatcggc
    15181 gacgcgacgg tgaagcagca ttacgggcga gccttgcgcg ccaagctcta cgaagcttgg
    15241 ccggtgaccg ctgccgggac ctcgcgcgtc gatcgcgccc agtggagccg gcagccttac
    15301 cgctcaggct ctccgccccg gttcggagcc cagccacggc ccggcggccg cttcgccaat
    15361 ccgcagaagc cgcgccccag cgacagcctg aagaggagcg gtctcgtcgg gccgggccac
    15421 gcgccttacc gggaggcgct gctgctgcgc acgcttctca atcacccgtg gctggtcgag
    15481 gagcacgcgg aagcgattgc cgggatagat ttcacgctga cggcgctcgg caggctccgt
    15541 gatgccatac tgtcactcaa aagcaacgac atttctcttg acagcagccg cttgcgcacc
    15601 caattggagg cgcagagctt gcaacaggcg gtcgacctcg tcgagcgggc gatcacccat
    15661 aagtgtgacc ggtttgcgga ggcagatgct gagagggccg atgtcgaggc tggctttcgc
    15721 catgcccttg cgctgcacga gcgccagaaa ggcctgaaga gagccctgga agccgcggag
    15781 cgggatttcc ttgaggaggg gagcgaagaa tcgctggcca ggatttgcga ggtacagcgc
    15841 cttatagcga gcttggacgt tgttgacgca ccgttggatt tttgagctgc ccaggtcaac
    15901 cagccggcag cacccgaggg ggtgtggccg cagtggcttg ggcatatgcg cgtgaccggc
    15961 cttggcgttg atccggaacc gaaccttgag ggttgcccgc tagacatgtt tcgcgaagct
    16021 cccagctcga tccggacccg ttcgcctgca gcgtgcacag ccctcccagc tgcactcgcc
    16081 gtcaccaaga agcccgcgac ctttgcgcca aactgataga acgggagcct tacgctgccc
    16141 atgtcgaatg gattctcttc cgcagagccc cgtacgcaga tgacacagaa aactgaagag
    16201 cagacgccac cccaggtcga aatgccggag cgcgacagtc cggtgctcga tctgtcggac
    16261 caggcggtga agcggctgat caagtccgcc aaggcgcgcg gctacgtgac gtatgacgag
    16321 ctgaatgcag tgctgccttc ggaggaggtc tcctcggagc agatcgagga cgtgatggcc
    16381 atgttctcgg acatggggat caacgtcatc gagagcgagg aaagcgaaga gtcgcctgac
    16441 gaggattccc tcgacgacga ggagggccgc gcgctcacca cgcagacgct gcccgcccgc
    16501 gccgagtcgc gcccggagcc ggtcgagcgc acggacgatc ctgtccgcat gtacctgcgc
    16561 gagatgggct cggtggagct gctctcccgc gagggcgaga tcgccatcgc caagcgcatc
    16621 gaggcgggcc gcgaggcgat gatcgcgggc ctctgcgaga gccccctcac cttccaggcc
    16681 atcatcatct ggcgggacga gctcaacgag ggcaaggtcc tgctgcgcga catcatcgat
    16741 ctcgaagcaa cctacgaagg tcctgacgcg aaggccgctc cggtcgaggc cgaggagggc
    16801 gacgaggagg acgaggccag ccgcgacggc gaggccaacg gtgccgcccg cggcgacatg
    16861 gacgatgacg acgattatga gaacgccatg tcgctggcgg ccatggaggc cgagctcaag
    16921 ccgaaggtcc tggagacctt cgaccgcatc gcgaatgcct acaaaaagct gcgcaaacag
    16981 caggatcgca acctggagat gcaggtcgcg ggcgagcagg gctcgcgcca gcagcagaag
    17041 gcctatgcca agctgcgcga ggagatcatc gcggacgtca agagcctgca gctcaacaac
    17101 aaccgcatcg agagcctcgt cgagcagctt tatgccatca acaagcggct ggtgggcctc
    17161 gaaggccagc tcatgcggct ggccgacagc tacggcgtcg cccggcagga tttcatcgac
    17221 gaatactacg gcaacgagct cgaccagacc tggctcgacc gcatcgcgaa gtccggcaag
    17281 aagggctggg cgaagttcat cgagaacgag cgcgcgcgca tcgaggacat ccgcggcgag
    17341 atccacaacc tggcgaccga gacgggcctc gagatcccgg agttccgccg cattgtgcgg
    17401 agcgtgcaga agggtgagcg cgaagcccgg caggccaaga aggagatggt ggaggccaac
    17461 ctgcgcctcg tcatctcgat cgccaagaag tacacgaacc gcggcctgca gttcctggac
    17521 cttatccaag agggcaacat cggcctgatg aaggcggtcg acaagttcga gtaccgccgc
    17581 ggctacaagt tctcgaccta cgccacgtgg tggatccggc aggcgatcac ccgctcgatc
    17641 gccgaccagg cgcgcaccat ccgcatcccc gtgcacatga tcgagacgat caacaagatc
    17701 gtgcgcacga gccgccagat gctgcatgag atcggccgcg agccgacgcc ggaggagctg
    17761 gccgagaagc tcgccatgcc actggagaag gtgcgtaagg ttctgaagat cgccaaggag
    17821 ccgatcagcc tcgagacgcc gatcggcgac gaggaggaca gccacctcgg cgacttcatc
    17881 gaggaccgca acgccgtcct gccgatcgac gccgcgatcc agtcgaacct gcgcgagacc
    17941 acgacccgcg tcctcgcctc cctcaccccg cgtgaggagc gcgtgctgcg catgcgcttc
    18001 ggcatcggca tgaacaccga tcacacgctg gaagaggtcg gccagcagtt ctcggtcacc
    18061 cgcgagcgca ttcgccagat cgaggcgaag gcactgcgga agctcaagca cccgagccgc
    18121 agccggaagc tcaggagctt tttggataat tgatcgtcgc ggcgatcacc gccaatcggc
    18181 accggaccga gccagcgccc caaacctccc tctccccggc ggggagaggg ttagggtgag
    18241 ggggttccgc cagtggcaac tgttgcaacc cctcacccgg cccttcggac cgacctctcc
    18301 ccatgggaga ggtaaggtgt cgccgctact accattccat ctggcgactc ggaaaatcaa
    18361 cgccgcagca gagatgttgc ggcgtttttc gtctggcgaa cctacctact ttttcgccgg
    18421 cgccgacgtc ttggtggcgg ccttctgctt ggctgcgggc tgcttggctg cgggcgcctc
    18481 cttctggccg gcagcagcag tggaggccgg cgcaggctgc atctgcacgg ccagaccgaa
    18541 cagccgccag tctcccccga cgttctcgaa caacatgtcg aagttgattt gctccggccg
    18601 agtctcgatg aagcctctaa ggcgcaggcg gccagcttcg tcgagggccg gctctcgaac
    18661 gagtttcgga ctgaagaaca gaatgggcgc gaaatcgagc tgccgatgcc gcaggtccgc
    18721 gaaagcttcc gtcagccgcg ccgccgaatt gacggactga aacgacggac tgccgagatc
    18781 gcgcaaaacc gtgtaattgc ccgtcaggtt tgcctgggac aaggcaatca gggtcgtccg
    18841 gatcagcatg tccattttgt agccgtcggg aagggcaggc tgcgtcgctt gctgcggcgg
    18901 ctgacttttg ccctgagcca gcgcgacgga gggctgagca agcacggcgg ctgtgatcga
    18961 cactgcacca cagatcagca gacgcatgat cgagcgattg ttcatcgatc ccctcgggac
    19021 gcaagcgtga gacaaggttt ccgttgcagt atcggtcatg tcgacgggaa cacacaacaa
    19081 cccgcagagc agtgacagca aacgttacac atcgcctagc tacaacgaaa aaaggacgca
    19141 cccgacaggg ccggatgcgt ccccagagga gtagaagcta ccggttacca gctgacctgc
    19201 aggccggcgc ggccaccgac gctgctcttg ctgcctcgac gaccatacga ctcttccttg
    19261 acggtgaagc cgacgccgcc gctcagagcc caacgctcgc ccgcaccgag gaaattgtgg
    19321 ccgaggacac ccatggcggt gaagccgagg gcgacgttgt cctcccagaa tgccacgttg
    19381 ccggcgagac cgaacttctc attaccgacg agatcggggt tctcgagggc catggcaatc
    19441 gccacgccgc cgccgatccg gctgagttcg tcgaagagca aaccaccgtc agtggccaag
    19501 tggccgttgg cgtcagaggt cacaacctcg agcggacctt gctggcgctg cctgctctcg
    19561 agagaagtga tgcccgctgc agtgtaggtg tggcgagccg tacctaaagc gaattcgtta
    19621 ggtaatgttg cctgagcacc ctggcccgta ttatccgttc caagcgcaac ggaaccatta
    19681 cccgtagcag aagtattgcg gccgagagcc attgagtttg aaccgcttgc ggtcgaaccc
    19741 ctgcccactg caagggaatt accgcctgat gcggcagaat cgcgaccaat ggcgatcgca
    19801 ttgtcagaag aggcaagtga gtccgttccc aatgcgatcg tatggttaat ctccaagggg
    19861 ttttcttgtg taccattgcc tgctcgcctt ccgatggcga ggttgtttac gcccgtaatc
    19921 caatttccag cttctcgccc gatcgctgtg ttgccagcgc cggcgatatc attcccagac
    19981 ttatccccta ttgcaacatt cccagcagac ggaacttcgt cattggtttg gtcattggga
    20041 tcatcaaacg tgccaagtcg gttgccagat tcggttccga tagcaacgtt tctgctgcca
    20101 gtgacgctat ttcctgaatc agaaccgaag accgagttcc gactgccgtt gatgttctta
    20161 ccagcatcat caccgactac agtgttcttt atgccaaccg cattagtacc agcatcctgg
    20221 ccgacgaaga tattggccca attattagcg gctgaaccct gacccgcatt ccgtccgatt
    20281 gaaatggagc gtgcgccaat cgcctgcgcg ttctggccaa ctgccgtcgc gccttcgcct
    20341 taagcggttg aataggctcc gacggtggtc gtccgatcac catctgccgt agccccacga
    20401 ccaatcgcag tcgccttttc accatccgcc gtgctttgac ggccgacagc agttgcttct
    20461 ttgccttccg cgagtgcccc actaccaact gcggttgtat catcaccgtc tgccgtggca
    20521 ctcggcccac atgcaaaagc agtcgagtcc actgaggtcg tggcaccacc attgccacct
    20581 gcggaaagtt cgcacgtatt ccccgccaaa gccgaacccg tgctcgcaat cgtcaatgcc
    20641 agcgcactgg ctgcgccgag caacacggcc ccgctcgctc ggcctgcaat tcttgatttg
    20701 aaatgtgtca tatctcgatg tcctatagac gggtcattgc aatgcccccc tcccatccag
    20761 ggaagcaggc acagttatgc tgtgctcgga tgaaagtgtg gtgatctttc tgccacggtt
    20821 ccgtgaaaag aatattcttg
//
LOCUS       DASVDU010000083         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1335742, whole genome shotgun sequence.
ACCESSION   DASVDU010000083 DASVDU010000000
VERSION     DASVDU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335742"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..2079
                     /locus_tag="VF226_05350"
     CDS             31..2079
                     /locus_tag="VF226_05350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7073448.1"
                     /translation="MSASSAANPLLGPWSTPYGAPPFAEIAPEHFPPAFEQALAEHRS
                     EIDRIAADPAAPTFDNTIVALENAGRLLDRVSAVFWNLASADTNDALQAIEREISPKL
                     AQHWNAITSNAALFARIDAVMQGAGSADLNPEQKRVLERTHLAFVRSGARLGPEEKKR
                     LGEIMERLAALGTQFSQNVLADEKSYELPLTEEDLVGLPDFLIDAARRAAEERGSAAK
                     FVITLSRSLIEPFLTFSPRRDLREKAFMAWTRRGENGGATDNRGIIAEMLNLRRERAR
                     LLGYETFADYKLDNTMAKTAANVRALLNDVWPAAKRRALQERDKLQAIAQREGANITI
                     EPWDWRYYAEKVRKAEYDLDESDVKPYLPLDNVIEAAFYTAGRLFGLSFSERPDVPVY
                     HPDVRAFEVTDSTGAHVGLFLGDYFARPSKRSGAWKSAYRRQERLAGDIRPIVVNVMN
                     FAKGSGDTPALLSFEDARTLFHEFGHALHGLLSNVTYPSISGTSVARDFVELPSQLFE
                     HWLLTPEVLGRFARHYRTEEPMPAEMIEKLRAASTFNQGFATVEYLSSAIVDIDFHSE
                     AYNGEEPIAFERAVLDRIGMPQEIVMRHRTPHFSHIFSGDGYSSGYYSYMWSEVLDAD
                     AFSAFEETGDVFDPETASRLKEFIYAAGGLRKEDEAYIAFRGRLPTVEGLLKKRGLLA
                     "
     gene            complement(2639..>3515)
                     /locus_tag="VF226_05355"
     CDS             complement(2639..>3515)
                     /locus_tag="VF226_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073449.1"
                     /translation="LAGLRSLPPPVAPIIEAPKKLVRHRSGRRVILKFAGLALTNRQL
                     WWDREVDPRRIANRAQVPVQAHCHGWEAKPWVEGFRLDAADASIAFVEGYLGPWIAAA
                     STKWLQPGEVQDGISRIAEALSAWAEMHDDRSSAALIEAIAERALAKVGSEPQPCYGD
                     GRLAPHEWIREPDGTVRKVDAGGHDCDHTWVGRQPIAWDLAGAEVEWSLGRRRTGALK
                     AIVQQLTGYSCSEETRAFYTAGYCAFRAAAARYSAMASRDADMRNHLCWAAEFYDRRL
                     KATLADVQMRHRQAA"
BASE COUNT          652 a         1141 c         1073 g          649 t
ORIGIN      
        1 acgatccagc ttccccgagg tctctgcacc atgagcgcca gttcggcagc caatcctttg
       61 ctcggcccct ggtccacgcc ctacggcgcg ccgccgttcg ccgagatagc gccggagcac
      121 tttccgccag cgttcgagca agccttggct gagcaccgat ccgagatcga ccgcattgcc
      181 gccgacccag cggcacccac cttcgacaac acgatcgtgg cgctcgagaa cgccggtcgc
      241 ctcctggacc gcgtttctgc cgtgttctgg aatcttgcca gcgcggatac caatgacgcg
      301 ctgcaggcca ttgagcgcga gatctcgccg aagctcgctc agcactggaa tgcgatcacc
      361 tccaacgcag ctctgtttgc gcgcatcgat gccgtaatgc agggcgcggg gtcggcggat
      421 ctcaaccccg agcaaaagcg ggttctggag cggacgcacc ttgcgttcgt gaggtcgggt
      481 gcgcggctcg ggcccgagga gaagaagcgt ctaggcgaga tcatggagcg gctggccgcc
      541 ctcggcacgc agttcagcca gaacgtcctc gctgacgaga agagctacga gctgccgctc
      601 acagaggagg atctcgtcgg cctgccggac ttcctgatcg atgcggcccg cagggcggcc
      661 gaggagcgcg gcagtgcggc caagttcgtc atcacgctct cccgatcgct gatcgagccg
      721 tttctgacgt tctcccctcg ccgcgacctg cgcgagaaag cgttcatggc ctggactcgc
      781 cgcggcgaga acgggggcgc caccgataat cgcggcatca tcgcggaaat gctgaatctg
      841 cggcgcgaac gcgcaaggct gctcggctac gagaccttcg ccgactacaa gctcgacaac
      901 accatggcca agacggccgc caacgtgcgg gcgcttttga acgacgtctg gccggcggcg
      961 aaaaggcgcg ccctgcaaga gcgcgacaag ctgcaggcca tcgctcagcg cgagggcgcc
     1021 aacatcacca tcgagccgtg ggactggcgc tattacgcgg agaaagtgcg caaggccgag
     1081 tacgatctcg atgagtcgga cgtgaagccc tacctgccgc tcgacaacgt catcgaggcg
     1141 gcgttctaca ccgcagggcg actgttcggc ctcagcttca gcgagcgccc tgacgtgccc
     1201 gtctatcacc cggacgtccg agcgttcgag gtgacggaca gcaccggcgc ccacgtcggc
     1261 cttttccttg gcgactactt cgcacgcccc tcgaagcggt ccggcgcctg gaaaagcgcg
     1321 taccgccggc aggagcggct cgcgggcgat atccggccca tcgtcgtcaa cgtcatgaac
     1381 ttcgccaagg gcagtggcga tactccggct ctgctgtcgt tcgaggatgc gcgcacgctg
     1441 ttccacgaat tcggacacgc ccttcatggg ctgctctcaa acgtgaccta tccctccatc
     1501 tcggggacct cggtggcgcg cgatttcgtc gagctgccct cgcagctttt cgagcactgg
     1561 ctgctgacgc cggaggttct gggccgcttt gcccgacact accggaccga ggagccgatg
     1621 ccagcggaga tgatcgagaa actgcgggcg gccagcacct tcaatcaggg tttcgccacc
     1681 gtggaatacc tgagctcggc gatcgtcgac atcgatttcc attcggaagc ctacaacggc
     1741 gaagagccga tcgccttcga gcgcgccgtg ctggaccgca tcggcatgcc gcaggagatc
     1801 gtcatgcggc accgcacgcc gcatttctcg cacatcttct ccggcgacgg ttattcctcc
     1861 ggctattaca gctacatgtg gtcggaggtt ctcgatgccg acgctttttc cgcctttgag
     1921 gaaaccggcg acgttttcga ccctgagacc gcctcccgcc tcaaggaatt catttacgcc
     1981 gccggcggcc tgcggaaaga ggacgaggct tacatagcct ttcgtggtcg gctgccgacc
     2041 gtcgaaggat tgctgaaaaa gcgcgggctg ttggcctgaa agctttcgaa aacgaacggg
     2101 ccgtttctcg ctttggcaga agggtcaaaa gcggtgtgcg cagggctgca tctctacgct
     2161 ccgccctgct tggtcggaag cggcattcta tagagaaaat aacgaaattg atttgacttt
     2221 cgctgccaca attgctcagc tcaaagcatc cttcgaaagg aggtctaaga aaatgtcacc
     2281 acctggcaga agtgagccat ctggcgcgtg ttgacgatcg cagcccgcgc aggatggcta
     2341 gatcagacaa tccgaattac tgcgcgatga ttgccggcat tggtgctggg ccggcgccgt
     2401 atcctgtttc tgggatttga gttgaactca tccggttact tttcttaaag caacaccgga
     2461 acccggcagg caagcgaata taggttcatc accgcaccgg caatgatcga aaccagatcg
     2521 tacggctagg gcagattacg gtgtcacgac cctggaggct gacccgaact cgtattgccc
     2581 ctgtgaccgc agatcggtcg agcccggcgg ttcgttcgct gggctcttct gttttgtctc
     2641 aagcggcttg gcggtgcctc atttgcacgt cagccagcgt cgccttcagc cgccgatcgt
     2701 agaattcggc agcccagcac aggtgattgc gcatgtctgc atcgcggctc gccatggcac
     2761 tatagcgggc cgccgccgcc ctgaaggcgc aatatccggc ggtgtagaat gcgcgcgttt
     2821 cctcagagca tgaatagccc gtgagctgtt gcacgatggc tttgagcgcc ccggtgcgcc
     2881 ggcggccaag gctccactcc acctcggccc ctgccagatc ccaggcgatc ggctggcgcc
     2941 cgacccaggt gtggtcgcaa tcgtgtcccc cggcgtcgac cttgcggacg gtcccgtccg
     3001 gctcacggat ccattcatgg ggtgcgagcc gtccgtcgcc atagcagggc tgcggctccg
     3061 agccgacctt cgcaagcgct cgttcggcaa tcgcctctat gagtgcggcg gaagaccgat
     3121 catcgtgcat ctcggcccag gccgagagcg cttctgctat tcgggatatc ccatcctgca
     3181 cctcgccagg ctgcagccac ttggtcgatg cagctgcgat ccacgggccg agataaccct
     3241 ccacgaacgc gatgcttgca tccgcggcgt ccaatcgaaa accttccacc cagggcttag
     3301 cttcccagcc atggcaatgc gcctggacag ggacttgtgc gcggttcgcg atacggcgtg
     3361 gatccacctc gcggtcccac caaagctggc ggttggtcag cgccaggccg gcgaacttca
     3421 ggatgacacg acgccccgac cggtgtcgga cgagcttctt cggcgcctcg atgatcgggg
     3481 cgacgggcgg aggcaaagat cgaagaccag caagc
//
LOCUS       DASVDU010000084        16578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1400199, whole genome shotgun sequence.
ACCESSION   DASVDU010000084 DASVDU010000000
VERSION     DASVDU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16578
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1400199"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="VF226_05360"
     CDS             complement(<1..101)
                     /locus_tag="VF226_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7073450.1"
                     /translation="MTYDLIVIGTGPGGYVCAIRAAQLGMKVAVVEKR"
     gene            complement(121..402)
                     /locus_tag="VF226_05365"
     CDS             complement(121..402)
                     /locus_tag="VF226_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073451.1"
                     /translation="MKLGNKIIEVEVLSFPVVKLSYSDGFTAVLDFTEKISWGEAVSP
                     LRDPEIFKTAHVGSGGASLEWIGPHGEEIDFCADALRFEAEALGTSAAE"
     gene            complement(665..1978)
                     /gene="odhB"
                     /locus_tag="VF226_05370"
     CDS             complement(665..1978)
                     /gene="odhB"
                     /locus_tag="VF226_05370"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEX7073452.1"
                     /translation="MAIEIRVPSLGESVTEATVGQWFKKPGDAVKADEPLCELETDKV
                     TVEVPAPAAGVLGNIEVQQGETVAVGALLGTIKEGAAAEKPAAPAPAAKAEPAPAPKP
                     APAKAQGETLEKAVPEAKGAMPPSPAARKIIEEKGLTPEQITGTGRRGQILKEDVLAA
                     AATAAPAAAPTAPPQPSAETIAFPVARAAPTPAPQMRAPSPPSDAAREERVRMTRLRQ
                     TIARRLKEAQNTAAMLTTFNDVDMSAVMSLRAQYKELFEKRHGVRLGFMGFFVKACIQ
                     ALREIPAVNAEIDGTDIIYKNYYHIGVAVSTDKGLVVPVIREADRLSLAEIEAQIADF
                     GRRARDGRLGIEEMQGGTFSITNGGVFGSLLSTPILNAPQSGILGMHRIEERPVVRDG
                     QIVIRPMMYLALSYDHRIVDGKEAVTFLVRVKELLEDPQRLVLEL"
     gene            complement(2024..4996)
                     /locus_tag="VF226_05375"
     CDS             complement(2024..4996)
                     /locus_tag="VF226_05375"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7073453.1"
                     /translation="MSRQALNEAFARTSFLHGVNAAYIEEMQALYEQNPGAVSDEWRL
                     FFQSLQEEKGAVEAEAQGPSWGRPLEELTQNGSSELVAALTGDYGEVEKRIRSKLQAN
                     AQAAGFELSVAASMRATQDSIRALMLIRAYRVMGHLVADLDPLGLNDRRTHRELKPET
                     YGFTEADLDRPIFIDKVLGLETATIRQILKILRRTYCRQIGVEFMHITSPVQKAWIQE
                     RIEGEGKDIKFTPEGRRAILNKLIEAETFEKFCEIKYTGTKRFGLEGGESMVPALEQI
                     IKRGGQLGVKEIVLGMAHRGRLNVLANVMSKPYRAIFNEFKGGSFKPDDVEGSGDVKY
                     HLGASSDRIFDGNSVHLSLTANPSHLEIVDPVVLGKVRAKQDQYGCPPHDRTAVLPLL
                     LHGDAAFAGQGVVAECFGLSGLKGHRTGGSIHFVINNQIGFTTNPRYSRSSPYCTDVA
                     KMVEAPIFHVNGDYPEAVVHVAKIATEFRQRFQKPVVIDMICYRRHGHNEADEPAFTQ
                     PLMYKTIKSHPTTLQIYSKRLIEEGVVTPDEVDEMRDRFRAKLEEEFKAADNYRPNKA
                     DWLDGRWAGLGRPDGEDLRGNTGVDLEVLKEVGRQITTVPADFNVHRTVRRLLDRRRE
                     MIETGEGIDWAMAEHLAFATLVKEGFPVRLSGQDVERGTFSQRHSVLIDQETERRYTP
                     LKHVSPDQAKFEVINSMLSEEAVLGFEYGYSLAEPHALVAWEAQFGDFANGAQVVIDQ
                     FISSGERKWLRMSGLVMLLPHGYEGQGPEHSSARLERYLQLSAEDNWQVANCTTPANY
                     FHILRRQLHRKVRKPLVLMTPKSLLRHKRVVSYLEELATGTSFHRVLWDGADRNHDHF
                     DASTVELKPDNEIRRVVLCSGKVYYDLYEMRHELGIDDVYLMRVEQLYPFPARALMAE
                     LGRFPNAEFVWCQEEPRNMGAWTFIEPNLEWVLQHIRAKHLRPRYVGRAAAASPATGQ
                     LSRHLQEQKTLVEEALKP"
     gene            complement(5154..6053)
                     /gene="sucD"
                     /locus_tag="VF226_05380"
     CDS             complement(5154..6053)
                     /gene="sucD"
                     /locus_tag="VF226_05380"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX7073454.1"
                     /translation="MAILVDRNTKVICQGITGSQGTFHTKQAKAYGTQIVAGVTPGKG
                     GSKHPDPELAEVPLFNTVAEAKAKTGANATSIYVPPPFAADAILEAIDAEIPLIVCIT
                     EGIPVMDMVKVKRALDGSASRLIGPNCPGVLTPDECKIGIMPGNIFKRGHVGIVSRSG
                     TLTYEAVKQTSDAGLGQSTAVGIGGDPVKGTEFIDVLEMFLADPDTHSIIMIGEIGGS
                     AEEDAAEFIKSEAAKGRKKPMAGFIAGVTAPPGRRMGHAGAIISGGKGKAEDKLEAMR
                     SAGIAIADSPAALGTTLAKVLNG"
     gene            complement(6427..6561)
                     /locus_tag="VF226_05385"
     CDS             complement(6427..6561)
                     /locus_tag="VF226_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073455.1"
                     /translation="MNSKILIIAAVLVVVAVAGYFYMTPEAEVTPTPPAATSEPANTQ
                     "
     gene            complement(6789..8288)
                     /locus_tag="VF226_05390"
     CDS             complement(6789..8288)
                     /locus_tag="VF226_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7073456.1"
                     /translation="MALAENPAAPAPRRAQMLIDGEWTDSAGGGEIVVESPGNRKPIA
                     SVPRGGAEDVDRAVAAAAKAFESWSRVVPRERGRMLQKIADAVEARVEELARIIAEET
                     GNALRTQARPEAKGAADIIRYFGGLASELKGETIPLGEHVLSYTRREPIGVVGAIIPW
                     NAPVLLAALKIAPALCAGNTLVLKAAEDAPLGVLWLAEVCQDHLPKGVLNVLTGYGEE
                     AGAALSNHPGIRKLSFTGSTEVGKLIMRAASERIVPVSLELGGKSPSIVYPDADEDWV
                     VDGIISAMRFTRQSQSCTAGSRLFLHADIFDSFLGRLAEKTSKLKIGDPLDESTDMGA
                     IINEKQFKKVCGYVEEGLRLPDAKLVMGGLPPKSGPLAEGYYALPTIFANAANDWRLA
                     REEIFGPVLVAIRWTDEEEAIRMANDTHYGLAAYVWTHDIGRALRTAHRIEAGWVQVN
                     QGLGQSPGHSYGGVKQSGIGREFSLEGMLESFTQRKNVTINLNTPAKVH"
     gene            complement(8429..8716)
                     /locus_tag="VF226_05395"
     CDS             complement(8429..8716)
                     /locus_tag="VF226_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073457.1"
                     /translation="MPKSRKRATRPPLAHEIFESDLAQDITGRNSLQGDDQQNVHNER
                     RAVPNERGEADDLLETYEKSDKDIRAKRDLGKGRRHSPEHPYNRPEEPEGE"
     gene            complement(8866..10686)
                     /locus_tag="VF226_05400"
     CDS             complement(8866..10686)
                     /locus_tag="VF226_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073458.1"
                     /translation="MAEAKASYSSKLGRVVDYLLGRNTLIGVASFMLLIISGYATWHG
                     MRDFIIGASSSSATQDLPGGQSISSDILVIAVVVALTFLMWLTLRETFGAGRSLRERL
                     ITFPLYVFLAIWSIGFGYGFWWSLISGEEATRTGLAGLQEDARDASAVIAARLDAVRA
                     QLDSVMSWSESQMAREETSGGSCGTASGAGRGPLYNARVGVRDAIVTMRDSMVRSWLE
                     PVQAEVEHLRETAAAIGGSTIEERQRAFEQRASEIRGRARSIAARSNQLGRSTAAEMR
                     ALANAVAIPPGQPGFSCYDPTLAERLRQAAAQAEQPAELKLREAVFSEGPAGVANAVK
                     NLWTNIGTYARSLAIYVASGGKEAFAHTESGEPITGRDLIALLATIGIDMGLLALAIL
                     NPPRQPPSIRPSGALMRQINDAIEMAVARAPNADREWIRRHFLHHNKASYLVIPNLYS
                     CDPNNPDEASKALAMNQLAGVLSDLDLVRWPKRGRWWLFEKDELQRLKKEESLGSDTD
                     LTEVRKAWLDKVGKADQESVSANDDLIRRATPLRNHGLFSKAERALQIAGWSERARSD
                     IEIFRLVDTEGLTPLLMVLNAGDPPRTPNIESGSEISSGA"
     gene            complement(10957..11907)
                     /locus_tag="VF226_05405"
     CDS             complement(10957..11907)
                     /locus_tag="VF226_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018245564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7073459.1"
                     /translation="MSPSPSLRRHKDGRRRERQPPQSLDELRNLAVLIRREQSAISLG
                     AKALDVLARLVDAPEQAAVRTISELGDLLSVNASTLTRLAQKLGFAGFNELQDIFREA
                     IADDGRYFYSRQAGRLLSSRQEASEDLAVFEKLAHETAANVNGFLSQLEADTVENAAT
                     LLARARRVRVHGVRQFHAFASFLTYGLGMLRSDVFLLDVPRLGIAEALAQMENGDVVV
                     VASCAPYTAEVAKVAAMAAAHGLKVIAITDTRASPLVPPSKFAFFIPHASSFFSNSMG
                     AYIVFCEALLNVVARKLGDRAMGALARRERLISDLQIETR"
     gene            complement(11886..12950)
                     /locus_tag="VF226_05410"
     CDS             complement(11886..12950)
                     /locus_tag="VF226_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX7073460.1"
                     /translation="MSTDVCSRLAVIGLSGTPYEVGLQLGRFGADVVRGHIVRTHAWA
                     SVMAFRDHPNLAAARELVATQFPRYWQEIRGLAHGLELPLEDVFAWNCRGDVWAMAPD
                     GCTTLQIPGQPSILAHNEDGDPGLKNRCAVAIIRAEGGRAFTAFVYPGSIPGHAFAVN
                     EAGLVQTVNNIRARSGTKIGVPRMVLTRAMLDCSSIEQAVDLLDRSERWGAFHLSLTQ
                     RGFPYILSIEFTRSKCSVRQIDQPSCHANHLIHDATAGEDQIVTASSRSRQERGDEVL
                     RLTCQKRPDPLAILWDKAKPTLPLYREQPDDPDGENTIATALFEVHPHRVSWRIFDRA
                     YTSAILCCDDGYEPCRPHLA"
     gene            complement(12940..13668)
                     /gene="glnQ"
                     /locus_tag="VF226_05415"
     CDS             complement(12940..13668)
                     /gene="glnQ"
                     /locus_tag="VF226_05415"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HEX7073461.1"
                     /translation="MTMVEFRKVTKRFGSVVVLNEVDLKIDNGEVVVLIGPSGSGKST
                     LLRCINALEGIDGGDLIVDGISVKAGRSQVRLVRQEAGMVFQQFNLFPQMTALENVAF
                     GPRRVRGVSRAEAFAQARELLAKVGLADRGDHYPSELSGGQQQRVAIARALAVKPKLM
                     LFDEPTSALDPELRHEVLMVMQSLAEEGMTMIVVTHEMAFARRVGTRLIFMENGKITV
                     DGDPATLLANAANPRLREFLQHVN"
     gene            complement(13665..14321)
                     /gene="glnP"
                     /locus_tag="VF226_05420"
     CDS             complement(13665..14321)
                     /gene="glnP"
                     /locus_tag="VF226_05420"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HEX7073462.1"
                     /translation="MDFDWSVIVDAMPSLLNGARLTILITLAGLAGGLVVGFIAGLMR
                     AYGNALLNGIAFAYIELIRGTPIVVQVMFIYFALPVLADIRINPMSAAITAIIVNAGA
                     YIAEIVRGSFLSVHKGLREAGLALGLPMWKVLVYIIGPVAFRRMIPPLGNQFIVSLKD
                     TSLFIVIGVGELTRQGQEIMAANFRAVEIWTAVAIFYLIMTGTLTLILRVTEKRMRML
                     "
     gene            complement(14385..15131)
                     /gene="glnH"
                     /locus_tag="VF226_05425"
     CDS             complement(14385..15131)
                     /gene="glnH"
                     /locus_tag="VF226_05425"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HEX7073463.1"
                     /translation="MKLRQLFFAALTAIFVSASIQTAQAADLVVATDTAFVPFEFKEG
                     DKYVGFDIDMWDEIAKEIGVTYKLQPMDFNGIIPALQTKQVDVALAGITIKDERKKVI
                     DFSDGYYDSGFLLMVPADSSIKGPEDLKGKTLAVKTGTSAADYAKEHFKETELRQFPN
                     IDNAYLELQTGRVDAAMHDTPNVLYYIATAGKGRVKAVGEQMMAHEYGIGFPKGSPLV
                     EKVNKALAKMKEDGRYNAIYTKWFGTEPKS"
     gene            complement(15374..16216)
                     /locus_tag="VF226_05430"
     CDS             complement(15374..16216)
                     /locus_tag="VF226_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073464.1"
                     /translation="MRKVIHAGLIAGAATVVLAGVSVTEVYAFEVSKVELGVADSGGS
                     SCPRDAKITAWAHTDGPGTVKFVIRNDSGGKTGVLSANAVKGPTGNYLATYSHTFKIT
                     TDVDIKYMAEVVGHPKISKWVPLKAKCGPQPRKNVTTSKSGAKPPARAVSDDDGPKPR
                     KTTSTKSSGGGPKAKHTSESEDKGKPTSGGGKPIPSGGGKPIPSGGKPIATCKPMISV
                     TRTLAVTRAGGLATARAAWQSTALAAHGAAYARWSNAKDQSSSCSRKGLTFNCTVKAR
                     PCKG"
     gene            complement(16310..>16578)
                     /locus_tag="VF226_05435"
     CDS             complement(16310..>16578)
                     /locus_tag="VF226_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073465.1"
                     /translation="RVISSRSVASSRITHPGCWLCRFGMNKGSFGRSPKKQFRTSILC
                     RHGVALFREACVSSKLSKFFSHALEPFGVRERRIGTAAANERLM"
BASE COUNT         2930 a         5191 c         5139 g         3318 t
ORIGIN      
        1 cgcttttcga ccacagccac cttcatgccg agctgcgccg cgcgaatggc gcacacgtac
       61 cccccggggc cggtgccgat gacgataaga tcgtaggtca ttcttttttg agccttgaga
      121 ctattccgct gccgaggtac cgagcgcctc ggcctcgaat cgaagcgcat cggcacagaa
      181 gtctatttct tcgccatgcg ggccgatcca ttccaagctg gctccaccgg agccgacgtg
      241 ggccgttttg aagatttcag gatcgcggag gggcgataca gcttcgcccc aggagatctt
      301 ctcggtaaag tccagtacag cagtgaagcc atcgctatag ctgagcttca ctacgggaaa
      361 gctgaggact tcgacctcga ttattttgtt acccagcttc attcgatcca acctgggtcc
      421 agtttcacgc tggcgcgccg ccagcactct gttagccggc tttcgtgccg cttagcccat
      481 tcgagcaccg cattcaactt gttcggcggc aaagaccctg caagcacctc gtataagcct
      541 aatgtaatca cgaccgaatc atctccaatt cgggcgtgaa agtgtggtgg agggtgatcc
      601 tgataaaaga attggatttc actccgtcca caatagcgac agtcggcatg ccgcgccgac
      661 cgcctcaaag ctccagcacc agccgctgcg ggtcctccag aagctccttc acgcgcacca
      721 ggaacgtaac ggcttccttt ccgtcgacga tccgatgatc gtagctgagc gccagataca
      781 tcatcggtcg tatgacgatc tggccatcac ggacgaccgg gcgctcctcg atccggtgca
      841 tgccgagaat tccggattgc ggtgcattga gaattggcgt cgacaggagc gagccgaaca
      901 cgccgccgtt ggtgatcgag aacgtgccgc cctgcatctc ctcgatgccg aggcggccat
      961 cgcgggcgcg gcgcccgaaa tcggcgatct gtgcctcgat ctcggccagc gacaggcggt
     1021 cggcctcgcg tatgacgggc acgaccaacc ccttatccgt gctcaccgca acgccgatgt
     1081 ggtaatagtt cttatagatg atgtctgtgc cgtcgatctc ggcattgacc gccggaatct
     1141 cgcgcagcgc ctggatgcag gccttcacga aaaaacccat gaagccgagg cgcacgccgt
     1201 ggcgcttctc gaaaagctcc ttgtactgcg cgcgcaggct catcacggcg ctcatgtcga
     1261 cgtcgttgaa cgtcgtcagc atggcggcgg tgttctgcgc ctccttgagc cggcgggcga
     1321 tggtctggcg cagccgcgtc atgcgcacgc gctcctcgcg ggccgcgtcg ctgggcgggg
     1381 atggcgcacg catctgtggc gccggcgtag gcgcggcgcg tgcaaccggg aaagcgatgg
     1441 tttcagcgga gggctgcggc ggggccgtcg gcgcagctgc cggtgcagct gttgctgcgg
     1501 ctgccagaac gtcctccttc aggatctgcc cgcggcggcc cgtgccggta atctgctccg
     1561 gcgtgaggcc cttctcctcg atgatcttgc gggcggctgg tgaaggcggc atggcgcctt
     1621 tcgcctcggg cactgccttc tccagcgtct cgccctgcgc ttttgcggga gccggcttgg
     1681 gcgccggtgc aggttcggcc ttggcggccg gagcaggagc ggctggcttc tcggcggcag
     1741 cgccttcctt gatcgtgccc agcaaggcac cgacggccac ggtttccccc tgctgaacct
     1801 cgatgttgcc cagaacgccc gcggccggag ccggcacctc gacggtcacc ttgtccgtct
     1861 ccagctcgca gaggggctcg tcggccttga cggcgtcgcc cggcttcttg aaccactgac
     1921 ccaccgtcgc ctcggtgacg ctctcgccga gcgacggtac ccgaatttca atcgccattt
     1981 ctcttcccgg ccctcactat ccgccgattt ttgcccccgc cagtcacggc ttgagagctt
     2041 cctcgaccaa tgtcttctgc tcctgcaggt gtcgcgaaag ctggcctgtc gccggcgatg
     2101 cggcggcggc ccggccgacg tagcgcggcc gcaggtgctt cgcgcggatg tgctgcagga
     2161 cccattccag gttcggttcg atgaaggtcc aggcccccat gttgcggggc tcctcctggc
     2221 accagacgaa ctccgcgttg ggaaaacgcc cgagctccgc cataagcgcg cgcgccggga
     2281 agggatagag ctgctcgacg cgcatcagat agacgtcgtc gatgccgagc tcatgccgca
     2341 tctcgtaaag gtcatagtag acctttccag agcacagcac gacccgacgg atttcgttgt
     2401 ccggcttgag ctccaccgtg gacgcgtcga aatgatcgtg gttccggtct gccccgtccc
     2461 agagcacgcg atggaagctg gtcccggtcg ctaattcttc aagataggag accacccgct
     2521 tgtggcgcag gagcgacttc ggcgtcatca gcaccagcgg cttgcgtacc ttgcggtgaa
     2581 gctgccggcg caggatgtgg aagtagttgg ccggcgtggt gcagttcgcg acctgccagt
     2641 tgtcctccgc cgaaagctga agataacgct ccaagcgggc ggaggagtgc tccggtccct
     2701 ggccctcgta gccgtggggc agcagcatca ccaggcccga catcctgagc cacttgcgct
     2761 cgcccgacga gatgaactga tcgatgacga cctgtgcgcc gttggcgaag tcgccgaact
     2821 gcgcctccca ggcgacgagc gcatgcggct cggcgaggct gtatccgtac tcgaacccga
     2881 gcacggcttc ctccgacagc atcgagttga tgacctcgaa cttcgcctgg tcgggggaaa
     2941 cgtgcttcag gggcgtgtac cggcgctccg tctcctggtc gatgagaacg gaatggcgct
     3001 gtgagaaggt gccgcgctcc acgtcctgcc ctgagaggcg gacgggaaag ccttccttga
     3061 ccagtgtggc aaaggccaag tgctcggcca tcgcccagtc gatgccttcg ccggtttcga
     3121 tcatctcgcg acgccggtcg agcaggcgac ggacagtccg gtgcacgttg aagtcggcag
     3181 gaacggtcgt gatctgccgc ccgacctcct tcagaacctc cagatcaacg ccggtgttgc
     3241 cgcggagatc ctcaccgtcg ggccggccga gcccggccca gcggccgtcg agccagtcgg
     3301 ccttgttcgg acggtagttg tcagcggcct tgaactcctc ctcgagcttg gcgcggaagc
     3361 ggtcgcgcat ctcgtcgacc tcgtcgggcg tgacgacgcc ctcctcgatc aggcgcttcg
     3421 agtagatctg cagcgtcgtt ggatgcgact tgatggtctt gtacatcagc ggctgcgtga
     3481 acgccggctc gtcggcctcg ttgtggccgt gccgccggta gcagatcatg tcgatcacga
     3541 ccggcttctg gaaccgctgg cggaactccg tcgcgatctt cgcaacgtgc accacggctt
     3601 cgggatagtc gccgttgacg tggaagatcg gcgcctcgac catcttggcg acatcggtgc
     3661 agtagggcga ggatcgcgag tagcgcggat tggtcgtaaa gccgatctgg ttgttgatga
     3721 cgaaatggat cgacccgccg gtgcgatggc ccttgaggcc cgacaatccg aagcattccg
     3781 caacgactcc ctggccggca aacgccgcat cgccgtgcag cagaagcggt agcacggccg
     3841 tgcggtcgtg tggcgggcag ccgtattggt cctgcttggc gcgcaccttg cccagaacca
     3901 ccggatcgac gatctcgagg tgcgacgggt tggccgtcag cgacagatgc accgagttgc
     3961 cgtcgaagat gcgatcggag gaggcgccga ggtggtactt gacgtcgcca gagccctcga
     4021 catcgtccgg cttgaacgag ccgcctttga actcgttgaa gatcgcccgg taaggcttgc
     4081 tcatgacgtt ggcaagcacg ttgagccgac cgcgatgggc catgccgagc acgatctcct
     4141 tcacgccgag ctgtccgccg cgcttgatga tctgctccag cgccggcacc atcgattcgc
     4201 cgccctcgag gccgaagcgc ttcgtgccgg tgtacttgat ctcgcagaac ttctcgaatg
     4261 tttccgcctc gatcagcttg ttgaggatcg cccggcggcc ttcgggggtg aacttgatgt
     4321 ccttgccctc gccctcgatg cgctcctgga tccaggcctt ctgcacgggg gaggtgatgt
     4381 gcatgaactc gacgccgatc tggcggcagt aggttcgtcg caggatcttg aggatctgcc
     4441 ggatcgtcgc cgtttcgagg ccgagcacct tgtcgatgaa gatcggccga tcgaggtccg
     4501 cctcggtgaa gccgtaggtc tcgggcttca gctcgcgatg ggtgcggcgg tcgttgagcc
     4561 cgagcggatc gaggtcggcg acgaggtggc ccatcacgcg ataggcgcgg atcagcatca
     4621 gcgcccggat cgaatcctgg gtggcccgca tcgaggccgc gacagaaagc tcaaagccgg
     4681 ctgcctgcgc gttcgcctgc agcttgctgc gaatgcgctt ctcgacctcg ccatagtcgc
     4741 ctgtcagggc cgcaaccagc tcggagctgc cgttctgggt cagctcttcg agtggccggc
     4801 cccaggaagg gccttgcgcc tcggcctcga ctgctccctt ctcctcctgc aggctctgaa
     4861 aaaagagccg ccactcatcg cttacggctc ccgggttctg ctcatagagc gcctgcatct
     4921 cctcgatgta ggcagcattg acgccgtgca ggaatgacgt ccgtgcgaag gcctcgttca
     4981 gcgcttgtcg tgacatttcc aacttggtcc ggactgtgcg gttcgcacca cgcgaacgca
     5041 atgaagcgcc cacgcggggc gcctattagc acatcggctg atttagtaca cgcacgccca
     5101 ttgtttaagg gtgacaccat gttcccgccc catgtctgac acccgggggt tactcagccg
     5161 ttgagaacct ttgcgagcgt ggtcccgagg gccgcagggc tgtccgcgat ggcgatgccg
     5221 gcggagcgca tggcttccag cttgtcctcg gccttgccct tgccgccgga gatgatggcc
     5281 cccgcgtgcc ccatgcgccg cccgggcgga gccgtgacgc ctgcgatgaa gcccgccatc
     5341 ggcttcttgc ggcccttggc ggcctcgctc ttgatgaact cagcggcgtc ttcctcggcc
     5401 gaaccgccga tctcgccgat catgatgata gaatgcgtgt cggggtcggc gaggaacatc
     5461 tcgagcacgt cgatgaactc ggtgcccttc accgggtcac caccgatccc gaccgccgtc
     5521 gactgaccga gcccggcgtc cgaggtctgc ttgacggcct cataggtgag cgtgcccgag
     5581 cgcgagacga tgccgacgtg gccgcgcttg aagatgttgc cgggcatgat gccgatcttg
     5641 cactcgtccg gcgtcagcac gccgggacag ttgggaccga tgagccgcga ggcagagccg
     5701 tccagcgcac gcttgacctt caccatgtcc atcaccggga tgccctccgt gatgcacacg
     5761 atgagcggga tctcggcgtc gatggcttcc agaatggcat cggcggcgaa gggaggcggc
     5821 acgtagatcg aggtggcgtt ggcgcccgtc ttggccttgg cttcggcaac cgtattgaac
     5881 agcggcacct cggccagctc cggatcggga tgcttcgagc cgcctttgcc gggggtcacg
     5941 cccgcaacga tctgcgtgcc ataggccttg gcctgcttag tgtgaaaggt gccttggctg
     6001 ccggtgatgc cttgacagat gaccttggta tttctgtcga cgagaatggc catgagcgtg
     6061 ccttctcctg cgctcttgaa gttccgccgt gcttgtcgga cagaatggtc gcgcgatcaa
     6121 gtgcgttgcg acaagtggaa cggggcagat cggcccgcgg cgcagctgcg cgcgctgctg
     6181 cgagtcgagc ggattgcggc tggctcgtta ccatcaggcg cggcggcgct gcctgcggcc
     6241 cgtgcttatt ggaaccactg gccggcggct cagtcgagat cgctcacctg cctgacgagg
     6301 cgttaacatc tttcaggggc gcgctcatcc cgccgcacgc cggtgacgcc cgcaccacgc
     6361 agatacagcg cccggcatca accatcgccg ggcggactgg tgcggaacca atgcgcagtg
     6421 cagcgattat tgcgtgtttg cgggctcgct ggtcgccgcc ggaggagtgg gcgtcacctc
     6481 ggcctccggc gtcatataga agtagccagc gaccgccaca acgacgagca ctgccgcaat
     6541 gatcaggatc ttgctgttca ttgtgcgtcc tccgagttga accggacgcg tggctaccgc
     6601 ctcaatttgt tcagaacagg accgtgaaat gaacagctcc atgctggccg tcgccgctaa
     6661 ccggcagccg gccacggcgg ggcggggtcg cgctcgctcc ctcgctgtcg ccaactggtg
     6721 aggcgcacca atgcggatta tccagagtcc ggctcgcgcc ggctgccgcg cccgtgcggc
     6781 agcttcgctc agtgcacctt cgctggcgtg ttcaggttga tggtcacgtt cttgcgttgc
     6841 gtgaagctct ccagcatgcc ttccagcgag aactcccggc cgatgccgct ttgcttgacc
     6901 ccgccgtagg agtgacctgg tgactgccca aggccctggt tgacctgcac ccagccggcc
     6961 tcgatgcgat gggccgtgcg cagggcgcgg ccgatgtcgt gagtccagac gtaggccgct
     7021 aggccataat gcgtgtcgtt ggccatgcgg atcgcctcct cctcgtccgt ccagcggatg
     7081 gcgaccagca ccgggccaaa gatctcctcg cgtgccagcc gccagtcgtt ggcggcattg
     7141 gcgaagatgg tgggcagcgc gtagtagcct tcagcgagcg ggcctgattt cggtggcagg
     7201 cctcccatca ccagcttggc gtcgggcagt cggagccctt cctcgacgta gccgcaaacc
     7261 ttcttgaact gcttttcgtt gatgatcgcc cccatgtcgg tcgattcatc cagcggatcg
     7321 ccgatcttga gcttcgacgt cttctccgcc aaccgaccga ggaagctgtc gaagatgtcg
     7381 gcgtgaagga acagccgcga ccccgccgtg caagactggc tctgccgcgt gaaccgcatg
     7441 gcggaaatga tgccgtccac gacccagtcc tcgtcggcgt cggggtagac gatcgacggg
     7501 ctctttccgc cgagctccag cgacaccggc acgatgcgct cggaggccgc gcgcatgatg
     7561 agcttgccga cctccgtcga gccggtgaac gagagcttgc ggatgccggg atgattggac
     7621 agcgctgcgc ccgcttcctc gccgtagccg gtgagcacgt tgagcacgcc cttcggcagg
     7681 tgatcctggc acacttcggc gagccacagg acgccgaggg gcgcatcttc ggctgccttg
     7741 aggaccagcg tgttgccggc gcagagggct ggcgcgatct tgagcgcggc aagcagaacg
     7801 ggcgcgttcc aggggatgat cgccccgacg acgccgatcg gctcgcggcg cgtgtagctc
     7861 agaacatgct cgccgagtgg gatggtctcg cctttcagct cgctagcgag gccgccgaag
     7921 tagcggatga tatcggccgc gcccttcgcc tccgggcgcg cctgcgtccg gagcgcattg
     7981 cccgtttcct cggcgatgat gcgggccagc tcctcgacac gcgcttcaac cgcatcggcg
     8041 atcttctgca gcatgcggcc gcgctcgcgc ggaacgacgc ggctccagct ctcgaacgcc
     8101 ttggccgcgg ccgcgactgc gcggtcgacg tcttcggccc cgccgcgcgg gacgctggcg
     8161 atcggcttgc ggttgccagg actctcgacg acgatctcgc caccgccggc gctgtcggtc
     8221 cactcgccgt cgatcaacat ctgggcgcgc cggggtgcag gcgcagccgg attctcagcc
     8281 agggccatgt ggaacttctc cttcgctgcc cgaaggcagc agtcgtgtgc tgaactgctc
     8341 ggtgcacaat aggcccatcg cctcggatcg tgaagcgtct ggctcggttc tcgtaatgaa
     8401 tgaggatagg gagaccgggc cggctactct actctccttc cggctcctcc ggcctgttgt
     8461 agggatgctc cggtgagtgg cgacggccct tgccgagatc ccgtttggcg cggatgtcct
     8521 tgtcggactt ctcgtaggtt tccaacaggt cgtctgcctc tccgcgctcg ttgggtacgg
     8581 cgcgacgctc gttgtgcacg ttctgctggt cgtcaccctg cagggaattc cttcccgtta
     8641 tgtcttgcgc gagatccgat tcgaagatct cgtgcgccag cggtggccgc gtcgcacgtt
     8701 tccgggattt tggcatgtcc ccgcatctcc ttctcgatgc tcgaaagaaa acacgggcgg
     8761 cgaacctcgg ttccttgcca gggcttggct gggtggatcc cgcggtgctg gggagccgtg
     8821 ttgcgagact ttggaatgtt gcgagacttt ggaataagcg tcatctcaag cgcccgagct
     8881 gatctcgctg ccgctctcta tgttaggtgt gcgcggcgga tcgccggcat tcaagaccat
     8941 cagcagcggc gtcagcccct ctgtgtcaac gaggcggaaa atctcgatat cgctacgcgc
     9001 gcgctcgctc cagccagcga tctgcaaggc gcgctctgct ttcgagaaaa ggccgtgatt
     9061 gcgcagcggc gttgcccggc gaatcaaatc atcgttggca ctcacgctct cctgatcggc
     9121 cttgccgacc ttgtcgagcc atgccttgcg cacctcggtg aggtccgtgt cactgccgag
     9181 actttcctcc ttctttagcc tctgcaactc gtccttctcg aacagccacc accgcccgcg
     9241 cttcggccag cggaccaaat cgagatcgct taacacaccg gcgagctggt tcatcgccag
     9301 cgctttcgaa gcctcgtccg ggttgttggg atcgcagctg tagaggttcg gaatgaccag
     9361 atacgatgcc ttgttgtgat ggaggaagtg gcggcggatc cactcgcgat ccgcgttcgg
     9421 cgcgcgtgct acggccatct cgatcgcgtc gttgatctga cgcatcagag cgcccgaggg
     9481 acggattgag ggcggctgcc gcggcgggtt caggattgcg agcgccagca aacccatatc
     9541 gatgccgatc gtggcgagca gcgcgatcag atcgcgcccg gtgatcggtt ctccactttc
     9601 tgtgtgcgcg aaggcctctt tcccgccgga ggcaacgtag atggcaaggc tgcgcgcgta
     9661 tgtgccgatg ttcgtccaga ggttcttgac ggcattcgca acgccggccg gcccttcgct
     9721 gaacactgcc tcacgcagct tgagctcggc aggctgctcg gcctgggccg cagcctggcg
     9781 caggcgctct gccaaggtcg gatcgtagca actgaaaccc ggctgccctg gtggaatcgc
     9841 aaccgcattg gcgagcgcac gcatttccgc cgccgtcgat cgcccgagct gattgctgcg
     9901 ggcggcaatg ctgcgcgcgc ggccgcggat ttcggacgct ctttgctcga acgcgcgctg
     9961 gcgctcctcg atggtgctgc cgccgatggc cgccgccgtc tcgcgcaggt gctcaacctc
    10021 cgcctgaaca ggctcgagcc atgagcgaac catgctgtca cgcatggtca cgatggcatc
    10081 gcgaacgccg acacgggcat tgtagagtgg cccgcgaccg gcgcccgagg ccgtgccgca
    10141 actgccgccg ctcgtctcct cgcgggccat ctggctttcc gaccagctca tgacactgtc
    10201 gagctgggcc cgaacggcat cgagccgggc ggcaatgacg gcgctggcgt ctcgcgcatc
    10261 ctcctgcagg ccggcgaggc ctgtgcgggt cgcctcctcg cccgagatca ggctccacca
    10321 gaacccgtag ccgaagccga tcgaccagat ggcaaggaac acgtaaagcg gaaatgtgat
    10381 gaggcgctcg cggaggctcc ggccggcgcc gaacgtctcg cgcagcgtga gccacatgag
    10441 aaacgtcaga gcgacgacga ccgcgatcac gagaatgtcg ctcgagatcg actggcctcc
    10501 gggtagatcc tgcgtagcgc tgctcgagga cgctccgatg atgaagtcgc gcatgccgtg
    10561 ccatgtggca tacccggaga tgatcagcag catgaaggat gccacgccga tcagcgtgtt
    10621 gcgaccgagc agatagtcga ccacccggcc caatttcgag gaatagctcg ccttcgcctc
    10681 ggccatttcc gcaactcccg gccctcttgc gtcagacgga agtgtaagga ggaccgcagg
    10741 actcgtgcga ccactgccgg ttgcaccccg tcagttctgc tgccgatgtt gcttgtcaat
    10801 cacagatgga caagggcgga gttggaaccg agcggcgcga caacaggttg actacgtcgc
    10861 gtattcctca acgcgctctt tccggtaggg gttgagcgat caaggttcaa cggactagcg
    10921 gcggcgagcg gggctcgtcg agccctcgtt cccgcttcac cgcgtttcaa tctgcaagtc
    10981 cgagatcagc ctttcacgcc gcgccagggc gcccatggcg cgatcaccaa gcttccgcgc
    11041 aacgacattg agcaatgcct cgcagaatac gatgtatgcg cccatgctat tgctgaaaaa
    11101 actgctagcg tgcggaatga agaaagcaaa tttggacggc ggcacgagcg gcgacgctct
    11161 cgtatcagta attgcgatca ctttcaggcc gtgcgcggca gccatcgcag cgactttcgc
    11221 gacctccgca gtatagggcg cgcagctggc gacaacgacg acatccccgt tttccatctg
    11281 cgctaacgct tcggcgatgc ccagtcgtgg tacatccagc aagaagacgt cgcttcgcag
    11341 catgccgagt ccgtaagtca ggaagctcgc gaacgcatga aactgccgca cgccatgaac
    11401 tcgcacgcgg cgagctcttg ctaacaacgt cgcggcgttt tccaccgtgt cagcttcgag
    11461 ctggcttaga aatccgttga cattggctgc cgtttcgtgg gccagtttct cgaacacagc
    11521 caagtcctcg ctggcctcct gccttgacga aagtaaccgt cctgcctgac ggctgtagaa
    11581 gtagcgcccg tcatcggcga tggcctcgcg aaatatgtct tgaagttcat tgaatccggc
    11641 gaatccaagc ttttgagcga ggcgcgtgag cgtggaagcg ttcaccgaga ggaggtcgcc
    11701 gagttccgaa attgtccgaa cagcggcctg ctccggagca tcgacgagcc gggcgagaac
    11761 gtcaagcgcc ttagcgccaa gtgaaatggc tgactgctca cgcctgatca gaacggcaag
    11821 atttcgaagc tcatccaggc tttgcggcgg ctgacgctcc cgccgtcttc catccttgtg
    11881 acgtctcagg ctaggtgagg gcgacacggt tcgtatccat catcgcagca gagaatggct
    11941 gacgtgtaag cacgatcgaa aatgcgccac gacacccggt gcggatgaac ttcgaataag
    12001 gcggtggcga tggtgttctc gccgtctgga tcatccggct gttcgcgata gagtggcagg
    12061 gtaggcttgg ctttatccca caagattgcg agcggatcgg gtctcttctg acaagtgagg
    12121 cggaggactt catcaccacg ttcctgacgc gatcgtgagg aggcggtgac gatctgatct
    12181 tcgcctgccg ttgcgtcgtg aatgagatga ttggcatggc agctcggctg atcgatttgc
    12241 cggaccgaac atttgcttcg ggtgaactcg atactcaaaa tgtacggaaa gccgcgctgc
    12301 gtgaggctca agtgaaaggc accccaacgc tcacttctat cgagaagatc gacagcctgc
    12361 tcgatgctac tgcagtcgag catggcccgt gtcagcacca ttcggggaac gcctatttta
    12421 gtgccactgc gcgcacggat attgtttacg gtctgaacaa gtcccgcctc gttgaccgcg
    12481 aaggcatgcc cggggatgga gccgggatag acgaatgcag taaaggccct tccgccttca
    12541 gctcgaatga tggcaacggc gcatcggttc ttcaggccgg gatcgccgtc ttcattgtgc
    12601 gcaaggatcg atggctgacc gggaatctgc agcgtggtgc agccgtctgg tgccatggcc
    12661 caaacatcac cgcggcaatt ccaggcgaaa acgtcttcga gggggagttc gaggccgtgc
    12721 gccaagcctc tgatctcctg ccagtagcgc ggaaactgtg ttgctaccag ctctcttgca
    12781 gctgcgagat ttggatgatc gcgaaaggcc atgacgctag cccatgcatg agtccttacg
    12841 atatgacctc taacgacatc tgcaccaaac ctccccagtt gaaggccgac ttcgtacggc
    12901 gttccggaaa gaccgatgac tgccagccgc gagcagacgt cagttgacat gctggagaaa
    12961 ctctcgcagg cggggatttg cggcattggc gagcagtgtc gcggggtcgc cgtctacagt
    13021 gatcttgccg ttctccatga aaatcagccg cgtgccaact ctacgtgcaa atgccatttc
    13081 gtgggtgacc acgatcatgg tcatgccttc ctcggcgaga gactgcatga ccatcaaaac
    13141 ctcgtggcgc agttccgggt cgagcgcgga cgtgggctcg tcgaacagca tgagcttcgg
    13201 cttgacagcg agcgcacgcg caatcgcgac gcgttgctgc tgtccaccgg aaagctccga
    13261 ggggtagtga tcaccgcgat cggctaatcc gaccttggcg agcagctcgc gggcctgagc
    13321 aaatgcttca gcccgactca cgccgcgcac gcgccgggga ccaaaagcaa cgttctccaa
    13381 cgccgtcatc tgcggaaaaa gattgaactg ctggaaaacc atgccggctt cctgccggac
    13441 caagcgcact tgcgagcggc cggccttgac gctgatgcca tcgacgatga gatctccacc
    13501 gtcgatccct tctagggcat tgatgcagcg cagcagagtc gacttgccgg agcccgaggg
    13561 accaatcaag accaccacct cgccgttgtc gatcttgagg tcgacttcgt tcaggacgac
    13621 gacgctgccg aaccgcttcg tgacctttcg aaactctacc attgtcatag cattctcatc
    13681 ctcttttcgg tcacgcggag gatgagcgtg agggttccgg tcatgatcag gtagaaaatg
    13741 gcaacggccg tccaaatctc gactgccctg aaattggcgg ccatgatctc ctgaccttgc
    13801 cgggtcaact cgccgacgcc gatgacgatg aagagcgaag tgtccttcaa gctgacgatg
    13861 aactgattgc cgaggggcgg aatcatccgg cggaaggcga ccgggccgat gatgtaaacc
    13921 agcaccttcc acatcggcag accgagcgcg agcccagctt ccctgaggcc cttgtgaacg
    13981 gagaggaatg aaccgcggac aatctcagca atataggcgc ccgcatttac gattatcgcc
    14041 gtgatcgctg cgctcatcgg attgatgcgg atatccgcca gcaccggcag tgcaaagtag
    14101 atgaacatga cttgcacgac gatgggcgtt ccgcgaatca gctcgatgta agcaaacgcg
    14161 atgccgttca acagggcatt gccataggcc ctcatcagtc cagcgatgaa gccgaccacg
    14221 aggccaccag ccaatccagc caaggtgatg agaatcgtaa gccgggcgcc gttgagcagc
    14281 gacggcatgg catcgacgat gacggaccaa tcgaaatcca tattgagctc cgaagggatt
    14341 tccgtgccgg attatttccg gcacggtcct tgagttttcg aggctcagct cttcggctcg
    14401 gtgccgaacc acttcgtgta gatggcgtta tagcgtccat cttctttcat cttggcgagc
    14461 gccttgttga ctttctccac gagcggactt cccttcggga agccgatgcc atactcgtgc
    14521 gccatcatct gctcgccaac ggctttgaca cggcccttac cggctgtagc gatgtagtag
    14581 aggacatttg gcgtatcgtg cattgcagca tcgacgcgac ccgtctgaag ctccaggtaa
    14641 gcgttgtcga tattcgggaa ctgacgcagc tcggtttcct tgaaatgctc tttggcgtaa
    14701 tcggctgccg aggtgccagt tttcaccgcg agtgttttcc ccttgagatc ctctggcccc
    14761 tttatgctgc tgtcagcggg caccatcagc aggaagccgc tgtcgtaata tccatcggag
    14821 aagtcgatca ccttcttgcg ctcgtctttg atggtgatgc ctgcaagcgc gacgtcgact
    14881 tgcttcgtct gcagggcggg gatgatgccg ttgaagtcca tcggctgcag cttgtaggtg
    14941 acgccgatct ccttggcgat ctcgtcccac atgtcgatgt cgaagccgac gtacttgtca
    15001 ccttccttga actcaaaggg gacgaatgct gtgtcggtgg ccaccaccag gtcggcggct
    15061 tgtgccgttt gaattgaggc agatacgaaa attgccgtga gtgctgcgaa gaagagctgt
    15121 cggagtttca tgatgcggta cccctattct gaagcgcgcc ttgcagtgtt gcacaggaga
    15181 gtgcaaatct gcaatgcttt cttttgcgcg atgccgccaa aatttgcgag gtgctgcgcg
    15241 tgatagcgtg cggccgcgag cctttccccg cgcgaatgtc cagcacacat cagatagatg
    15301 ccggcaaaga atgcaccagc ccgacatccc ggactggtgc atctgcggac ggcgacgttt
    15361 cgtgtcggcc ggtctagccc ttgcagggcc gcgccttgac cgtgcagttg aaagtgagac
    15421 ccttgcggga gcagctcgag ctctggtctt tggcattgga ccaccgtgca taagcggcgc
    15481 cgtgtgcggc gagcgcagtc gactgccagg cggcccgagc agtcgcgaga ccgcctgccc
    15541 gcgttacggc aagggttctc gttaccgaga tcattggctt gcaggtggcg atcggctttc
    15601 caccagaagg aatgggcttg ccaccgccgg acggaatcgg cttgccgcca ccggacgtgg
    15661 gcttgccctt atcctcgctc tccgatgtgt gctttgcctt gggacccccg cccgacgact
    15721 tggtcgaggt ggtcttcctc ggcttcggac cgtcatcatc ggagacggct ctcgccggtg
    15781 gctttgcgcc tgacttagag gtcgtcacgt tctttcgagg ttgcggaccg catttcgcct
    15841 tgagcggtac ccacttcgag atcttcggat gtccaacgac ttccgccatg tacttgatgt
    15901 cgacatccgt ggtgatcttg aacgtatggg agtaggtggc gagataattc cccgttgggc
    15961 ccttgaccgc gttcgccgat agaacgcccg tcttgccgcc actatcattg cgaatgacga
    16021 atttcacagt gcccggtccg tctgtgtgcg cccacgcggt gatcttcgca tcacgcgggc
    16081 aactgctgcc gccgctgtcg gcgacgccga gctcgacctt gctcacctcg aatgcgtaaa
    16141 cctcggtgac ggatacgccg gcgagcacca ctgtcgcggc cccggcaatc aatccggcat
    16201 gaatgacttt gcgcatggtt ttccttctcc cttttccgcc ttggtatttg ccgttgcatc
    16261 tcacacggaa gatttgttca ctgcgtgtct attcggacgg ttcgcagcac tacatcagac
    16321 gttcattcgc tgcggccgta ccgatgcgtc gctcccggac gccaaaaggt tcaagtgcgt
    16381 gtgagaaaaa ttttgatagc ttgctgctca cgcacgcttc tcgaaatagc gcgacgccgt
    16441 gacggcacag gatgctcgtt ctaaactgct ttttggggga acggccaaat gaacctttgt
    16501 tcatgccgaa gcgacataac cagcagccag ggtgcgtgat gcgcgaagaa gcgactgacc
    16561 gggagctgat gacccgga
//
LOCUS       DASVDU010000085         4722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_753635, whole genome shotgun sequence.
ACCESSION   DASVDU010000085 DASVDU010000000
VERSION     DASVDU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753635"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VF226_05440"
     CDS             complement(<1..200)
                     /locus_tag="VF226_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter"
                     /protein_id="HEX7073466.1"
                     /translation="MRTRLLAFALAALAAAGLATAPAVAQDKSIVVSSTTSTQDSGLF
                     GHILPMFKEKTGIEVKVVAQGTG"
     gene            complement(217..969)
                     /locus_tag="VF226_05445"
     CDS             complement(217..969)
                     /locus_tag="VF226_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7073467.1"
                     /translation="MLDKAAQNASAAAGVFGSDLPICFTNVDYRVGSVSILCGINLEI
                     AGGPPTLLLGPNGSGKTTLLKLAMGLITPTAGSITYGGRDSPAPGTRAFVFQKPMMLR
                     RTVAANVAFALKAAGRRPDRLEIIRLLAEVGLEGMDDRPARRLSGGEQQRLALARALA
                     RDPEVLFLDEPTASLDPASTKAVEDIVARVAKSGVKIVMTTHDLGQARRLASEVVFLA
                     KGRLVERAPVEAFFTKPATDEARRFVAGELVI"
     gene            complement(959..1648)
                     /locus_tag="VF226_05450"
     CDS             complement(959..1648)
                     /locus_tag="VF226_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073468.1"
                     /translation="MDTSSALELILAGDATLFAIVRLSLLVTLSATFFSALIGLPLGA
                     VVALTNFPGRHAVIVVLNGLMGLPPVVVGLLVFLLLSRSGPLGALGLLFTPTAMVIAQ
                     TVLITPIIAALSRQVIEDLWTEYRDELTAMEVGPLARVKTLLWDARFSLVTILLAGFG
                     RAAAEVGAVMIVGGNIENFTRVMTTTIALETSKGNLPLAIGLGMVLITLILVVNALAW
                     GVRRWSEAHAG"
     gene            1738..2631
                     /locus_tag="VF226_05455"
     CDS             1738..2631
                     /locus_tag="VF226_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7073469.1"
                     /translation="MNAGLELLTTAEAAEYLRLKERKLYELVAERQIPCTKVTGKWLF
                     PRADLDRWLLAGMMRPHGVVPADPPPIVGGSHDPLLQWALSESRAGLAIMPEGSEAGY
                     RRFLRGEVIAAAIHFHDLDDPEKDANFEIVSREPTLYDAVLIGFAAREQGLLVLPGNP
                     LGLMSIRDVLYKKARLVVRPEGAGAQQLLLALLKKEGAQLKDVAATLEAPTGPDIAQA
                     IRAGHGDCGVATRAVATSAGLDFVPLLMERFDLLMRQRDAFRPQLQKLLKLLGTSQFA
                     TRAAELGGLDISGAGSVRWAP"
     gene            complement(2721..3353)
                     /locus_tag="VF226_05460"
     CDS             complement(2721..3353)
                     /locus_tag="VF226_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7073470.1"
                     /translation="MAKGRPRKFDTNEALDKALIVFWQRGFEGTSLSDLTEAMGINRP
                     SLYAAFGDKETLFRRALDRYAEVGPAAVLHGALQQPTARGVIEHLLRGVAEAFTHPDR
                     PAGCLVVQGALSCGETAEAVKQELCCRRASAEAQLRERLERAKAEGDLPPDADTAILS
                     RYVSIVVKGMSVQAADGASRAELLAVVDMVLKCWPFGRATPVADGEQQLQ"
     gene            3716..>4722
                     /locus_tag="VF226_05465"
     CDS             3716..>4722
                     /locus_tag="VF226_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7073471.1"
                     /translation="MTHSLKTSIGGLVAISAIVAVGLVALPGSGPHADGTSSLPPPPP
                     SVSVAVVEPRDVTIWDEFSGRLEAVERVAVRSRVAGTVQQVHFREGALVSKGDLLITI
                     DPEPYAAEVERFEAQVAAAEARVEFTRTEVERGRRLLGTSVLSERDVDQRTNAYREAE
                     ANLRAAKAALQSARLNLEYTEVRAPVSGRVGRLEVTVGNLIAAGPSAPVLTTIVSVDP
                     IYASFNAGENVVARALSALAPEARAAADVGQIPVEISNISTGGSWLPGRLQLIDNQVD
                     AESGTVRVRAVFENRDGRLIPGQFARIRMAQPKSSPVLAISERAIGTDQDKRFVMAVD
                     E"
BASE COUNT          901 a         1527 c         1501 g          793 t
ORIGIN      
        1 cccgtgccct gcgcgacgac cttcacttcg atgccggtct tctccttgaa catcggcagt
       61 atgtggccga agaggccgga gtcctgcgtc gaggtcgtcg acgagacgac gatggacttg
      121 tcctgcgcga ccgcaggcgc ggtcgccaga cccgcggctg ccaaagcggc aagtgcgaat
      181 gccagaagcc gagtgcgcat ttgagctccc cttgggttag atgacgagtt cgcccgccac
      241 gaaccggcgc gcctcgtcgg ttgcaggttt cgtgaagaag gcctcgaccg gcgcgcgctc
      301 gacgagccgg cctttggcga gaaagaccac ctcggatgcc agtctcctgg cctgtccaag
      361 atcgtgggtg gtcatcacga tcttgacgcc cgacttcgca acacgggcga cgatgtcctc
      421 gaccgccttg gtcgaggcgg gatcgaggct tgccgtcggc tcgtcgagga acagcacctc
      481 gggatcgcgg gcgagcgcgc gggccagcgc cagtcgctgc tgctcaccac cggacagcct
      541 gcgcgccggg cggtcgtcca taccctcaag gccgacctcg gcaaggagcc ttatgatctc
      601 cagccggtcc ggccgccggc ccgcagcctt gagcgcaaag gcgacgttgg cggcaaccgt
      661 cctccgcagc atcatcggct tctggaagac gaaggcgcgc gtgcccggtg ccggactatc
      721 gcggccgccg tacgtgatgg acccggccgt gggcgtgatg aggcccatgg cgagcttgag
      781 cagggtcgtc ttgcccgaac cgttggggcc gagcagaagt gtcggcggac cgcctgcaat
      841 ctccagattg atcccgcaga ggatggagac cgacccaacc cggtaatcga cgttcgtgaa
      901 gcaaatgggc aaatcgcttc cgaatacgcc agcggctgcg cttgcgttct gtgccgcttt
      961 atccagcatg agcctctgac cagcgtcgaa caccccaagc caatgcattg acgaccagga
     1021 tcagcgtgat cagcaccatg ccgagaccga tggcgagcgg caggttgccc ttggaggtct
     1081 cgagcgcgat ggtcgtcgtc atcacgcgcg tgaagttctc gatgttgccg ccgacgatca
     1141 tcaccgcccc gacctccgcc gcagcgcggc caaagcctgc gagcaggatc gtcaccagcg
     1201 agaagcgggc atcccagagc agcgtcttca cccgcgcgag cgggccgacc tccatggccg
     1261 tcagctcgtc ccggtactcg gtccacagat cctcgatgac ctgacggctg agcgcggcga
     1321 tgatgggcgt gatgagtacc gtttgcgcaa tcaccatcgc cgtaggggtg aagagcaacc
     1381 ccagggcacc gagcggcccc gaccgcgaga gcagaaggaa gacgagcagg cccacgacga
     1441 ccggcggcag gcccatcagc ccattgagca cgacgatgac ggcgtgccgg cccggaaagt
     1501 tcgtgagggc aacgaccgca cccagaggca agccgatgag ggccgagaag aaagtggccg
     1561 aaagagtgac gagcagcgaa agacgcacga tggcgaacag cgtcgcatcg cccgccagaa
     1621 tcagctccag agcgctggat gtatccaagc acctctcccc aatgcccatt gaccaaagat
     1681 attgagtgtg tttggtctca gaatgtcaaa ttgtagaatc catgcagtcc aatgcagatg
     1741 aacgcaggtc tagagcttct gacgaccgcg gaagccgctg aatacctccg gctcaaggag
     1801 cgcaagctct acgagctcgt agcggagcgt cagatcccgt gtacgaaggt gacgggcaaa
     1861 tggctgttcc ctcgcgccga tctcgaccgc tggctgcttg cgggaatgat gcgcccacac
     1921 ggcgtcgttc cggcagatcc cccaccgatt gtgggcggga gccatgaccc actcctgcaa
     1981 tgggcgctaa gcgaaagccg cgctgggctg gcgatcatgc ccgaggggag cgaagccggt
     2041 tatcgccgct tcctgagagg ggaagtcatc gctgcagcga ttcattttca cgatctcgac
     2101 gatcccgaaa aagatgcaaa cttcgagatc gtgtctcggg agccgacgct ctacgatgcg
     2161 gtgctaatcg gctttgccgc gcgggagcag gggctgctgg tcctgcccgg caatccgctc
     2221 gggctcatgt cgatcaggga tgtgctctac aagaaggcgc gcctcgtggt tcgccccgaa
     2281 ggcgcgggag cccagcaact cctgttggcc ctcctcaaga aggagggagc ccaactgaag
     2341 gacgtcgcgg caacgctcga ggcgccaacg ggaccggaca tcgctcaggc catcagggcc
     2401 ggccatggcg actgtggtgt tgcaacgcgg gccgttgcga cctctgccgg gctcgacttc
     2461 gttccacttc tcatggaacg cttcgacctc ctcatgcgcc agcgcgatgc cttccgccca
     2521 cagctgcaaa agctgctgaa gctgttgggg acatcacagt ttgcaacgcg tgctgcggaa
     2581 cttggaggtt tggacatctc gggcgccggc agcgtgcgat gggcgccttg aggaatgtga
     2641 aggaaactaa tgatgcttta ttccgcttcc gcaccgaatg gcagcaagct ctaataggga
     2701 ggagacctcc aaagacggct ctattgcaat tgctgctctc cgtccgcaac tggagtggcc
     2761 cggccgaaag gccagcactt cagcaccatg tcgaccacgg ccagcagctc cgcccgtgat
     2821 gctccgtcgg ctgcctgtac cgacatcccc ttgaccacga tcgacacgta acgcgacagg
     2881 attgcggtgt cggcatcggg cgggagatcg ccctccgcct tcgcgcgctc cagtcgttcc
     2941 cggagctgcg cttcggctga ggcacggcga cagcacagct cctgcttcac ggcttcggcc
     3001 gtctctccgc aagacagcgc accctgaaca acaagacagc ccgcaggacg atcagggtgg
     3061 gtgaaggctt cggcaacacc gcgcagcaga tgctcgatga cgccgcgagc ggtcggttgc
     3121 tgcaacgccc cgtgcagtac ggcagcgggc ccaacctcgg cgtagcggtc gagcgcgcgg
     3181 cgaaagagcg tctccttgtc tccgaacgcc gcataaaggc tcggccggtt gatgcccatc
     3241 gcctccgtga gatcggacaa cgatgttcct tcgaacccac gctgccagaa aacaatgagt
     3301 gccttgtcca gcgcctcgtt ggtatcgaat tttctagggc gacccttggc caccatcttc
     3361 acctcttctc gctgtgcaga cacactatag ctttttcata ctcatcggta cagataacta
     3421 ttgacacacc cggcgtcacc tccctattac gtaccgctcg gtacacagaa agtggcgagg
     3481 cacacgatgg cactcacgtt agcatgtcag tgaagccagg ttccggaatg acctctggag
     3541 aagagggcgt ccggtgccga ccatgcgaga ctcaccggtg agcctccgcc gactgggtcc
     3601 gaccagataa acccgcaact ggcgcagatc cgcttatcac aagaagacct cactcgagct
     3661 ggggagcccc atttcccggc aggagcggat tgtgctctga ccaaacgaga tgatcatgac
     3721 gcattctctc aagacctcga tcggcggcct ggtggcgatc tccgccatcg tggccgttgg
     3781 cctcgtcgcg ctccccggca gtggaccgca cgcggacgga acctcctcac ttccccctcc
     3841 gccgccgagc gtctcggtgg cggtggtcga gccgcgcgac gtcactatct gggacgagtt
     3901 ctcgggccgg ctcgaagccg tcgagcgcgt cgccgtgcgc tcgcgggtcg ccggaacggt
     3961 ccagcaggtc catttccggg aaggcgctct cgtgtcgaaa ggcgaccttt tgatcaccat
     4021 cgatccggag ccgtacgcgg ccgaggttga gcgcttcgaa gcgcaggtcg cagcggctga
     4081 ggcccgggtc gagttcacga gaaccgaggt cgagcgtggg cgccggctcc tcggcacgtc
     4141 cgtgctgtcg gagcgcgacg tggaccagcg caccaacgcg taccgggaag ccgaagccaa
     4201 cctgcgcgct gcaaaagcag cgctgcagtc ggcacgtctc aatctggaat acaccgaggt
     4261 gcgcgcgccc gtctcgggac gcgttggccg gctggaggtc accgtcggca atctgatcgc
     4321 ggcaggtccg agcgctcccg ttctgacgac gatcgtgtcg gtcgacccga tctacgccag
     4381 cttcaatgcc ggcgagaacg tcgtcgcgcg tgcgctctcc gctctcgccc ccgaggcgag
     4441 agccgccgcg gacgtgggcc agatcccggt cgagatatcg aacatctcga cgggtggatc
     4501 ctggctgccg ggccggctgc agctcatcga caaccaggtg gacgcagaga gcggcacggt
     4561 gcgcgtgcgg gccgtcttcg aaaaccgtga cgggcgcctt atcccggggc agttcgcgcg
     4621 gatccgcatg gcgcagccga agagctcgcc ggtgctcgcg atcagcgagc gtgcgatcgg
     4681 cacggatcag gacaagcggt tcgtgatggc cgtcgacgag ga
//
LOCUS       DASVDU010000086         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_593332, whole genome shotgun sequence.
ACCESSION   DASVDU010000086 DASVDU010000000
VERSION     DASVDU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593332"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VF226_05470"
     CDS             <1..637
                     /locus_tag="VF226_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7073472.1"
                     /translation="EKQVMLKIYGRTNSINVRKVLWTAEEIGLTYTREDWGRGFRSTS
                     EPEFLKLNPFAVVPVIDDDGFVLRESHAISRYLAAKHGRTDLYPNDPKQRAQVEAWMD
                     WGQTDLYQGIRAPFLGMVVKLPAFQDQKLIDSGIAEWTRQMQMLDGCLAKGGPYLMGD
                     SFTLADIPVGLVVNRWFSVPFDKPELKAVSAYYEKLSERPAYRQHGRNGTP"
     gene            776..1267
                     /locus_tag="VF226_05475"
     CDS             776..1267
                     /locus_tag="VF226_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073473.1"
                     /translation="MAAAAAPATFATEVCVTCSGPPANYRCTVDEASKIEGYRYGKKA
                     LQFICITELAREGGHEKCSVKRTGSDICMGQERTVSLKGSIQALSAPMDSEAEAEPGS
                     VTGAEQPVSDQPKKPEPPKTVEELARRTASASKEKLEKTGNTVGKAFKKGLGCIASLF
                     QDC"
     gene            1567..1809
                     /locus_tag="VF226_05480"
     CDS             1567..1809
                     /locus_tag="VF226_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073474.1"
                     /translation="MVWRSAVLAAAALAISGCAEQRGTAVFGYQATRPPEVTEFSPEM
                     EAQLRKKTLASKVLAAIALERVTGRKPDPSRLTELN"
     gene            complement(1984..2694)
                     /locus_tag="VF226_05485"
     CDS             complement(1984..2694)
                     /locus_tag="VF226_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7073475.1"
                     /translation="MAERPIELYYWPTPNGHKITIMLEECGLPYEVKYVNIMRGDQFK
                     PEFLKISPNNRMPAIIDPDGPGGEPISVFESGAILQYLGRKTGKFYPSDERARVEVEE
                     WLFWQVGGLGPMAGQCHHFRNYAQEKISYAIDRYVNEVNRLYGVMDTRLADRPYLAGD
                     YSIADMACYPWVRPYKNQGQDLAEFPNLEAWFKRIHDRPAVARAVQVGAEEREKWNLA
                     KDKEAQKILFGQRARKRG"
     gene            complement(2828..3703)
                     /locus_tag="VF226_05490"
     CDS             complement(2828..3703)
                     /locus_tag="VF226_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7073476.1"
                     /translation="MIVRGNESVLVMGITGKQGTFWAEKMRGYGTRIIGGTNPKRAGE
                     KHLDLPVWASARDAAKETQVDCAVMFIPPFGVKAAALDAIEAGIRKIVCLTEHVPVHD
                     TMYFLAAARERGAQVIGPNTAGLVTTGESFVGFMPAFNERVFKPGEVGVISRSGSLGT
                     LLCLNLVQAGLGISSFVGIGGDPIIGTTTRDALECLDRDPHTKAIALVGEIGGAMEEQ
                     AAEYARTMSKPVAAFIAGAASPPGKKMGHAGAIVMGNRGSYAAKKAALEAAGVIVCAT
                     PNGVPEALKARLAPA"
     gene            complement(3700..4833)
                     /locus_tag="VF226_05495"
     CDS             complement(3700..4833)
                     /locus_tag="VF226_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7073477.1"
                     /translation="MNFEEHAAKPLLRAVGIATPKGALATTPDEAERIASEIGPCVVK
                     AQVPTGKRGKAGGIKLAATPAEARAHAERILGMTIGEHRVERLLIEEQVPIAKEMYAA
                     VLNDPDSKGPLLLFSAEGGMDIEEIAERHPKALVRFSIDIRKGLDRDAFAAALPSELP
                     VDRAAILDALSKLYSAYAANDAELMEINPLVVTRDGRIIALDCKFVMDDSAIPRHEEL
                     AKSGTPDKLTELEARAKALGLKFIELDGDVGVLANGAGLTMTSMDAVRHYGGQPANFL
                     EIGGESYTKAKPALELVLANPRVKALLVNFCGAFARTDVMAEGVVTAWQQLKPDIPII
                     FTIHGTGEDEAIAMVKERLGIEPYDLMDDAVRAAVAAARGAAQ"
BASE COUNT          857 a         1577 c         1517 g          914 t
ORIGIN      
        1 ggagaagcaa gtcatgctga agatctacgg acggacgaac tccatcaacg tccgcaaggt
       61 gctatggacg gccgaggaga tcgggcttac ctacacccgg gaggattggg gccgcgggtt
      121 ccgttccacc tcggagccgg agttcctgaa actgaacccg ttcgccgtcg tgccggtgat
      181 cgacgacgac ggcttcgtcc tacgggaaag tcacgccatc tcgcgctatc tggcggcgaa
      241 gcatggccgc accgatctat atccgaacga tccgaagcaa cgtgcgcagg tcgaggcgtg
      301 gatggactgg ggacagacgg atctctacca gggcatccgc gcaccgttcc ttgggatggt
      361 cgtgaagttg cccgccttcc aggatcagaa gctcatcgac agcggcatcg ccgaatggac
      421 gcggcagatg cagatgctcg atggttgcct ggccaaaggc ggcccttatc tgatgggcga
      481 cagcttcacg ctcgccgata tcccagtcgg ccttgtcgtc aaccgctggt tctcagtccc
      541 gttcgacaag ccagagttga aggctgtaag cgcctactac gagaagcttt ccgagcgccc
      601 ggcttaccgt cagcacggcc gaaacggcac gccttgaccc gctgctcgac ggggcactca
      661 gcccaggtgc cccactgtcg ccccgttttc cgtttgagat cggtatgtta accgatcttc
      721 atgggaggct tgcgcgatcg tgatacggca ggctctttcc gctttcggaa tagcaatggc
      781 agcggccgca gcgcctgcca cctttgcgac cgaggtgtgc gtgacctgct ccggcccgcc
      841 agcgaattat cggtgcaccg tcgacgaggc gagcaagatc gagggctaca ggtacggcaa
      901 gaaggccctg cagttcatct gcatcaccga gctcgccagg gagggcgggc acgagaagtg
      961 cagcgtgaag cgtaccggat cggacatctg catggggcag gagcggaccg tatcgctgaa
     1021 aggttcgatc caggccctct ccgctcccat ggactctgag gccgaagccg agccagggag
     1081 cgtgaccggg gctgagcagc cggtttcaga tcagcccaaa aaacccgagc cgccgaagac
     1141 cgtcgaggag ctcgcgcggc gcacggcttc cgcctcgaag gagaagctcg agaagaccgg
     1201 caacactgtc ggcaaggcct tcaagaaggg cctcggctgc atcgcctcgc tcttccagga
     1261 ctgctgaccc tggaagagcc gcggctgcca cgactgcgcc ccggcacccg ctagctggaa
     1321 attgaacggt tgtcagtttg ggcgatcgca tgtcagaaat gtgcgatcgg ttggcgcgcg
     1381 tgggccgccg gcatcctgga tccacactcg aaagagcgcc gattcaggca ttcaggctcg
     1441 gttcagccgc gatccgacca tagttgcgtc aaggtttcga ttgggtaaag tggcgggccg
     1501 gcgttgagcc aagggcccgc gtggtcccag agggtagttg tggatcgact gggggagggc
     1561 gatttcatgg tgtggaggtc tgctgtgctc gccgctgctg cgctggccat ctccggttgc
     1621 gctgagcagc gtgggacggc agtcttcggc taccaggcca cacgtccccc cgaggtgact
     1681 gagttcagcc ctgaaatgga agcccagctc cgcaagaaga ctttggcgag caaggtgctc
     1741 gccgccattg cgctggagcg cgtgaccggc cgcaagcccg acccgtcccg cctgacggag
     1801 ctaaactgag aggggtggtt gggtcctaac gccgtttcga cctggcccaa ctcatcgaac
     1861 gcaggtcaag cgagggttcc gacgactgcg agggtcataa atcctcgccc gacgctcggc
     1921 gcccacctct ccagacggga aaggtgcatc cttttttgct gctacggctt cacctgctcg
     1981 gagttagccc cgcttccgcg cccgctgacc gaagaggatc ttctgcgcct ccttgtcctt
     2041 ggccaggttc cacttctcac gctcttccgc accgacttga accgcccgcg caaccgcagg
     2101 gcgatcatgg atgcgcttga accacgcttc gagattgggg aactcggcca gatcctgacc
     2161 ctggttcttg tagggcctga cccagggata acaggccatg tcggcaatcg agtagtcgcc
     2221 ggcgagatac ggccggtccg ccagccgggt gtccatgact ccgtagaggc gattgacctc
     2281 gttcacatac cgatcgatcg cgtaagatat cttctcctgg gcgtagttgc ggaagtgatg
     2341 gcattgcccc gccatcgggc caagcccgcc cacctgccag aacagccact cctcgacctc
     2401 gacgcgggcg cgttcgtccg atgggtagaa cttgccggtc ttgcgcccga ggtattgcag
     2461 gatggcgccc gactcgaaga cggagatcgg ctcgccgccg gggccgtcag ggtcgatgat
     2521 cgccggcatg cggttgttgg gcgagatctt caggaactca ggcttgaact gatcaccgcg
     2581 catgatgttg acgtacttca cctcgtacgg cagcccgcat tcctcaagca tgatcgtgat
     2641 cttgtgcccg tttggcgtcg gccagtagta gagctcgatc gggcgctcgg ccatgtcatc
     2701 tctcctctgg aatgtcttct gacattacgg ggccaaagcg gcgtgagccg gatggcaccc
     2761 atactgttat ctggtcgaca ttcggccaga ttgccaattg caagtccgtg aacgagcgct
     2821 gccgggatca ggccggggcg agacgcgctt tcagtgcctc cggcacaccg ttcggggtcg
     2881 cgcagacgat cacccctgca gcttccagcg cagccttctt ggcggcatac gagccccgat
     2941 tccccatgac gatggcaccg gcgtgcccca ttttcttgcc cggcggagag gccgctccgg
     3001 cgatgaacgc ggccacgggc ttcgacatgg tgcgcgcgta ctcggcagcc tgctcctcca
     3061 tcgcgccgcc gatctcgccg acgagcgcga tggccttcgt gtgcggatcg cggtcgaggc
     3121 attcaagggc atcccgcgtc gtcgtgccga tgatcggatc gccgccgatg ccgacgaacg
     3181 aggagatgcc tagccccgcc tgcacgaggt tgaggcacag cagcgtgccg agcgagcccg
     3241 accgcgaaat gacgcccacc tcgccaggct tgaaaacacg ctcattgaag gccggcatga
     3301 agccgacgaa ggactcgccc gtcgtgacga ggcccgcggt attcggaccg atcacttggg
     3361 ccccgcgctc gcgcgctgcg gccaggaagt acatcgtatc gtgcaccggc acgtgctcgg
     3421 ttaggcagac gatcttgcga ataccggcct cgatcgcatc gagcgcggca gccttcacgc
     3481 cgaacggcgg aatgaacatc accgcgcaat cgacttgcgt ctccttcgcc gcatcccgag
     3541 cgctggccca gaccggcaga tcgagatgtt tctcgccagc acgcttcggg ttggtgccgc
     3601 cgataatgcg cgtgccatag ccgcgcatct tttcggccca gaacgtgccc tgcttgcccg
     3661 tgatgcccat cacgagcacg gattcattgc ctcgaacgat cattgcgccg cccccctggc
     3721 ggccgccact gccgcgcgaa ccgcgtcgtc catgaggtcg tagggctcga tacccagccg
     3781 ctccttcacc attgcgatcg cctcgtcctc cccggtgccg tgaatggtga agatgatggg
     3841 gatgtcgggc ttcaattgct gccacgccgt aaccacaccc tcggccatga cgtcggtgcg
     3901 cgcgaaggcc ccgcagaaat tgacgagcag ggccttcacc cgcggattgg cgagcaccaa
     3961 ttcgagcgcc ggtttcgctt tcgtgtagct ctcgccgccg atctcgagga agttggccgg
     4021 ctggccgccg tagtggcgca cggcgtccat cgacgtcatt gtaagacccg cgccattcgc
     4081 cagcacaccg acatcgccgt cgagctcgat gaacttgagc cccagcgcct tggcccgcgc
     4141 ttccagctcg gtgagcttgt cgggtgtccc cgatttcgcg agctcttcgt ggcggggaat
     4201 agcgctgtcg tccatcacga acttgcagtc gagcgcaatt atccggccat cccgtgtgac
     4261 gacgagcgga ttaatctcca tgagctcggc atcgtttgct gcgtaggcgc tgtagagctt
     4321 ggacaacgcg tccaggatgg cggcgcgatc gacgggcagc tcgctaggca gcgccgcagc
     4381 gaacgcatcg cgatcgaggc ccttgcggat atcaatgctg aagcgaacca gagccttcgg
     4441 gtggcgctcg gctatttcct cgatatccat gccgccttcg gccgagaaca gcagcagcgg
     4501 acccttgctg tcgggatcat tgagcaccgc tgcgtacatc tccttcgcaa tcggcacctg
     4561 ttcctcgatc agcagccgct cgaccctatg ctcgccgatc gtcatgccca ggatgcgctc
     4621 ggcgtgcgcg cgggcttcgg ctggtgtggc tgcgagcttg atcccgcccg ccttgccgcg
     4681 cttgcccgtc ggcacttgcg ctttcaccac gcaggggccg atctcgctcg cgatgcgctc
     4741 ggcttcgtcg ggcgtggtcg cgagcgctcc tttcggcgtc gcgatgccga ctgcgcgaag
     4801 cagcggcttt gcggcgtgct cctcgaagtt catgaccgct tacttccccc acttcgccca
     4861 atagg
//
LOCUS       DASVDU010000087        41980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1713253, whole genome shotgun sequence.
ACCESSION   DASVDU010000087 DASVDU010000000
VERSION     DASVDU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 41980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41980
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713253"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VF226_05500"
     CDS             complement(<1..159)
                     /locus_tag="VF226_05500"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HEX7073478.1"
                     /translation="MHNGEIARDDISRRLIVALDVPTYAEASAIVTDLGDAVHFYKIG
                     LELLFDGGL"
     gene            complement(244..834)
                     /locus_tag="VF226_05505"
     CDS             complement(244..834)
                     /locus_tag="VF226_05505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX7073479.1"
                     /translation="MPHTPKLLFLAGSAREGSHNKQLARLAAQIADANGLAATLADLG
                     DYPMPIYDRDHEVKNGAPDNARKLKALFEVHDGIFIAAPEYNASITPLLKNALDWISR
                     VRDEGEPPLQVFKTRVFAISSASPGRFGGVRGLITLRQVLAVGLQALVLPEQVSVQHA
                     ADAFDEKGHLKDKAQQELLKRQIERLARAAKVLKGL"
     gene            914..2164
                     /locus_tag="VF226_05510"
     CDS             914..2164
                     /locus_tag="VF226_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEX7073480.1"
                     /translation="MELTSDERAIAGGARGEASAMAMRIVAETGRLLGADRLIPVHSA
                     HIDGCLYHGDAGVLFAEKLVELGGQVAVPATLNVGALDLLHPKRVRLDDRRRDMALRL
                     MRAYEALGCKPTWTCSPYQAGHRPPAGVDVAWGESNAVAFCNSVLGARTNRYGDFLDI
                     ACAIVGRAPRYGLHLPEHRRATMLVDTSLLSAALRNLDAFWPVLGAWIGRNAGSSIVA
                     IDGLQTFASEDRMKALGAAAASFGAVGLFHVIGVTPEAKTRADAFSGHDPEVTVQLTS
                     EMVRIARGWLSTSSSDQVDAVAVGSPHLSFEEMMTIADLVDGRRLSLPFYAHTGRHVL
                     KQLEERGKLADIEAAGVTVVVDTCIVVTPILPAKSGVLLTNSGKFANYAPGNTGWSVV
                     YGSLSECVETAVQGRLVRDEALWS"
     gene            2161..2577
                     /locus_tag="VF226_05515"
     CDS             2161..2577
                     /locus_tag="VF226_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEX7073481.1"
                     /translation="MSTEMIAGQVLYPGSAEGPLLKLTAPLSFWGGVDPATGEIILGS
                     HPERGRSVSGSILALADPIGSSSSSSVLLELIHNRVAPAAILLGTPDAILIVGCLVAR
                     ELGLKAPPILVVAREWLHTLPNGRLSVSDGMVRQIS"
     gene            complement(2701..3195)
                     /locus_tag="VF226_05520"
     CDS             complement(2701..3195)
                     /locus_tag="VF226_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7073482.1"
                     /translation="MEPEDEAYIGYIIADVARLLRTVCDRRVRELGLTRAQWLAVTRL
                     HRRPGLSQSEVADLLEVEKATACRLLDRLEAKKWIERRPDKKDRRVKRIYLTPRANRV
                     QASIAPMAESTVDDALSDLSMEERQQLLDMMVRIKGKLQTLAGHETESMGTAVPVEEE
                     VRAV"
     gene            complement(3323..4102)
                     /gene="hisF"
                     /locus_tag="VF226_05525"
     CDS             complement(3323..4102)
                     /gene="hisF"
                     /locus_tag="VF226_05525"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX7073483.1"
                     /translation="MLKIRIIPCLDVKDGRVVKGVQFVDLIDAGDPVEAAAAYDAAGA
                     DELCFLDITASHENRGTLLDIVQRTAERCFMPLTVGGGVRSVEDIRRLLEAGADKVSI
                     NTAAVKNPQFVREASEKFGAQCIVVAIDAKKVSQPGEADRWEIFTHGGRNPTGIDAVA
                     FAREVTALGAGEILLTSMDRDGTKSGFDVALTRAVADAVSVPVIASGGVGTLDHLVEG
                     VLLGHASAVLAASIFHFGTYTIGQAKRHMAAAGIPVRLDQT"
     gene            4195..4671
                     /locus_tag="VF226_05530"
     CDS             4195..4671
                     /locus_tag="VF226_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073484.1"
                     /translation="MAKDTSHDLQHVAEAVRRKRLAAEDIKLLREKIAALDEAGTEHR
                     FVKAALRNLKVAGTQLKDIQERNPGEPFDEKAFEQLPEHLRKRIITRLDNIAKRMSGL
                     AEQGKPAEYSDPAFRCLQDYETCRKQAKLSVVWCQLAMVVCLLRSALTLSIRHGAK"
     gene            complement(4640..5383)
                     /gene="hisA"
                     /locus_tag="VF226_05535"
     CDS             complement(4640..5383)
                     /gene="hisA"
                     /locus_tag="VF226_05535"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX7073485.1"
                     /translation="MILFPAIDLKDGQCVRLKLGEMDAATVFNDDPAAQARTFEAQGF
                     QWLHIVDLNGAFAGKPVNAGAVEAILAAVSLPVQLGGGIRDLATVEMWLGKGVRRVIL
                     GTAAVRDPALVREACRRFPGRIAVGIDAKGGKVAVEGWAEVSELTAIELARRFEDAGV
                     AAIIYTDIERDGVLKGLNLPATAELARATRIPVIASGGLASLADVEALLRPEYAMLEG
                     AITGRALYDGRLDAKAALALISRRAGSKG"
     gene            5494..5793
                     /locus_tag="VF226_05540"
     CDS             5494..5793
                     /locus_tag="VF226_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073486.1"
                     /translation="MNAAASEIETARRPLTEADAIDVWIARWLRARRIDLCRRYQCDP
                     RRLYEIWEGQRFPAARVKAWRIFQERYPGLTDRIDPGPHRRIPRGRHPDQLTLFE"
     gene            5948..6304
                     /locus_tag="VF226_05545"
     CDS             5948..6304
                     /locus_tag="VF226_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073487.1"
                     /translation="MRLFLISYDLARSRRNKHAIATAIMNLGQAWARPLETTWYVRAN
                     ASEHDIQVRLSDMLDPEDGLIVQAVNEEAVLVNTSLRWFRQRRQDADRPSNVIAFPAG
                     LIEPLVQPELPLARAS"
     gene            complement(6317..6970)
                     /gene="hisH"
                     /locus_tag="VF226_05550"
     CDS             complement(6317..6970)
                     /gene="hisH"
                     /locus_tag="VF226_05550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX7073488.1"
                     /translation="MQRVVIIDYGSGNLHSAHKAFERAAREADVPAEINVSARPEDVK
                     AADRIVLPGVGAFADCKRGLDGVPGMIEALAQTVVERARPFLGICVGMQLMATRGLEF
                     GETPGLGWIDGDVRAIAPADPTLKIPHMGWNTLTVINDHPLLAGIRTGPQGLHAYFVH
                     SYHLVPRDRSVVVAETDYGGPVTAIVAKGNTAGTQFHPEKSQTLGLQLIGNFLRWRP"
     gene            complement(6982..7479)
                     /locus_tag="VF226_05555"
     CDS             complement(6982..7479)
                     /locus_tag="VF226_05555"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEX7073489.1"
                     /translation="MQTYTVYEPPKPPEDRVKRAERMVFVRDGFSWMAALFAPLWMLV
                     HRMWLALFLYVVLMIALSVALIAVDVSPQWRTVAVIAVHLAIGFEAGSLRRWKLERRR
                     WRMMGAVIGPSRIECERRFFQAWLGEEYRDDGSVRIGPPPRQATADAPGPIAGADAGP
                     VASEA"
     gene            complement(7501..8097)
                     /gene="hisB"
                     /locus_tag="VF226_05560"
     CDS             complement(7501..8097)
                     /gene="hisB"
                     /locus_tag="VF226_05560"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX7073490.1"
                     /translation="MSRTATIERKTKETEVKVTIDLDGSGAFDIKTGIGFLDHMLEQL
                     ARHSLMDLKVHAKGDLNVDFHHTVEDTGIAIGQAVAMALGDKKGITRYADVHLAMDEA
                     LTRVAIDVSGRPYLVWNVTFSRPKLGDMDTELFREWFQAFAQNSGITLHIATLYGENN
                     HHIAETCYKGLARALRAAVAEDPRQAGRVPSTKGRLAG"
     gene            8241..8807
                     /gene="hslV"
                     /locus_tag="VF226_05565"
     CDS             8241..8807
                     /gene="hslV"
                     /locus_tag="VF226_05565"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEX7073491.1"
                     /translation="MRNPPSSFESTWHGTTILTVRKGGRVVIAGDGQVSLGNTVIKSK
                     ARKVRSVLRGDVIGGFAGATADAFTLFERLEAKLEQYPGQLMRSAVELAKDWRTDRYL
                     RRLEAMMIVADRTTTLVLTGTGDVLEPEHDVMGIGSGGMYALAAARALLDQPLDAEAI
                     ARRAMEIAADICVFTNRNLIVESLPSDR"
     gene            8864..9187
                     /locus_tag="VF226_05570"
     CDS             8864..9187
                     /locus_tag="VF226_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX7073492.1"
                     /translation="MLAWGYLLTAGICEVLATTAFRYAEGFTRLGPTLAFAGSAVASL
                     LLLQLSLSGIPLGTAYAVWTGIGAAGTAILGAMYFNEPTTTMRLFFLTLLIGSILGLK
                     LASSN"
     gene            9198..10511
                     /gene="hslU"
                     /locus_tag="VF226_05575"
     CDS             9198..10511
                     /gene="hslU"
                     /locus_tag="VF226_05575"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX7073493.1"
                     /translation="MTTFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRQQLSEDL
                     REEVQPKNILMIGPTGIGKTEISRRLAKLANAPFIKVEATKFTEVGYVGRDVESIIRD
                     LVEVGLALVRETKRREVRARAEKMAEERVLDALVGEHASAATREAFRRRLRANELNDK
                     EIEIKVTDTGGGIPMFELPGVPGGSIGAINLGDMLGKAFGGRQKTRRVTVKDSYEILI
                     AEESDKLLDQEQITQEAIRAVENNGIVFLDEIDKICSRSDGGRIGADVSREGVQRDLL
                     PLIEGTTVSTKYGPVKTDHVLFIASGAFHVAKPSDLLPELQGRLPIRVELKPLTREDF
                     RRILTEPQASLIKQYIALMATEGLTLEFTDDAIDAIADTAAQVNATVENIGARRLQTV
                     MERVLDDISFDASDRAGQTITVDAQYVRDRVGDLAKNTDLSKFIL"
     gene            10559..11125
                     /gene="thpR"
                     /locus_tag="VF226_05580"
     CDS             10559..11125
                     /gene="thpR"
                     /locus_tag="VF226_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX7073494.1"
                     /translation="MPRLFTGIELPDDVREALARLRQPLPGARWIEPENYHITLRFAG
                     DVDNRLAAEFAHALSEVEVQAFEVRFAGTGAFGGNEPRTLWARIEPTPELEALARAHE
                     RAARAAGLQPETRKFKPHVTIARLRNTSVDVVARYLQRQGGFRTEPFLVSRFALFSSK
                     PQVGGGPYVIEETYPLIGGDYAEYEDEY"
     gene            11167..11466
                     /locus_tag="VF226_05585"
     CDS             11167..11466
                     /locus_tag="VF226_05585"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7073495.1"
                     /translation="MAQKLSPEARAEALARLSGWQPVEGRDAIFRQFKFADFNAAFGF
                     MTRVALVAEKMNHHPEWTNVWNRVDVTLTSHDAGGLSARDIKLAEAMDRIAGTQA"
     gene            complement(11521..12306)
                     /locus_tag="VF226_05590"
     CDS             complement(11521..12306)
                     /locus_tag="VF226_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073496.1"
                     /translation="MKLGSDIAAVVTGGASGLGAATAAALAEKGVKVSLLDLNEAAGR
                     EHAQRIGGTFAPCDVTNEESVEAALAAVRKAHGQERILVNCAGIAIAKRVVRRVKDTG
                     AIEPHDLASFAKVIQVNLVGTFLMISKCAAGMLTLDPLGPDGERGVIINTASVAAQDG
                     QIGQVAYAASKGGVASMTLPVARDLSRDGVRVCSILPGLFRTPMFAGLPEEAQKSLAA
                     TVPFPSRLGDPSEYAALAIHICENVMLNGASIRLDGAIRLAPR"
     gene            12437..13468
                     /locus_tag="VF226_05595"
     CDS             12437..13468
                     /locus_tag="VF226_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX7073497.1"
                     /translation="MIDLYSDTSTRPTPGMRQAMANADVGDEQRGEDPTTTALEERIA
                     ALLGKEAAVFLPSGTMCNQIALLVHCRPGDEVIGAAGTHVFTSEAGGGSALAGAQTWP
                     IVCDRGIFDGSDLGAAIREPSRHSPRSRLVVVEQTVNRGGGAIWPVEKIEAVAELAHR
                     RGLAVHMDGARLMNAVVATGIPARVMAARCDSVWLDLSKGLGCPVGGVLAGSRDFIDE
                     AWRWKQRIGGAMRQSGILAAAGLYALDHHVERLADDHANAALLSERIREIPGVRLMAP
                     RPETNIVYFDTSGTGLTAKAVTEALLAENVRMGTSYGGMIRAVTHLDVSRDDVEAAAA
                     VLRRVLDRR"
     gene            complement(13485..15689)
                     /locus_tag="VF226_05600"
     CDS             complement(13485..15689)
                     /locus_tag="VF226_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX7073498.1"
                     /translation="MTRTSDGASAAGEAENTPAAVETPEPDRKIIEHYIEEEEGALNR
                     LGGLVGKAITTIAVGVSLFHIYAAYDIVPAYVLRPVHVACVLFLVFLLFPAVPSLRDR
                     IRWWDWLLAFASLGVIVYVLMQGEEFGDRAIMPEPMDVVVGIIFIVLLLEATRRATGF
                     IMPALAIVFILYALFGNHLGEPWTHRGYPLDRLVGHLYMTLEGIFGTTVDVSSSLIIL
                     FTIFGAVLQVSGAGKFFIDFSFAAMGGKRNSAGRAVVLSSFLLGGPSGSGVATTVTIG
                     TVAYPLLAKAGYEKNAAGGLLAAGGLGAIISPPVLGAAAFLIAEFLKISYFDVILMAT
                     IPTLLYYLAIWTMVEVDARKTGGTSDLMVEKGTLWRLLAGYWYHFGSLVSIIAFLVLG
                     FSPVLAVFWATMLAIGSSAIRPDTALVPRIVVGATIAVAAVLSAIGFADGNAYSLTVF
                     IALALTAAAGLFLPQIAPVAARLAQALRDGTTGVLSVASICATAGIIVGVVTLTGLAQ
                     RFADIIIGYAQGSLLLTTIFTAAIVWIVGLAVPVTASYIMCAVIAAPALIKLGVPDFA
                     AHMFIFYYAVLSEVSPPTALSPFAAAAITGGNPYITTLQSWKYTMPAFLVPFVFVVDP
                     HGVGLLLHMPEGRSWVDVVEITLVVAAGIVALAFALQRWMVKRNTLLETALFWAAGLL
                     LVFPVVLDAAIFTPLTGLQLTSDIPGFHVAWSVPFGLAFGALAVVLQRLRTA"
     gene            complement(15797..16762)
                     /locus_tag="VF226_05605"
     CDS             complement(15797..16762)
                     /locus_tag="VF226_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7073499.1"
                     /translation="MKKQFLMALVAMGLGVFATGPANAQDKQLTIATGGTGGVYYPLG
                     GGLGNILSKELKGVTATAEVTGGSVDNMKLVGSGRTDIAFSQVDAAWDAINGQGKFPK
                     KLPVKALAVLYPNHMHVVTVDGTGIEKIEDLKGKRVSTGSPGSATEVFAFRVLEAAGI
                     DPEKDIGKERLGVAESVNAIKDGKIQAFFWVGGLPTAAVTDLAATPNTKIKFLEIAHL
                     TPKMNEKYGPLYAEAVIPKETYSGMDQDANNITVWNILAVNDAMPEDLAYNITKITFE
                     KREELAKVHKEALNIKPELQSVKRSGIPFHPGALKYFNEKGIKVD"
     gene            complement(16859..17833)
                     /locus_tag="VF226_05610"
     CDS             complement(16859..17833)
                     /locus_tag="VF226_05610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX7073500.1"
                     /translation="MRAALCKTLAGPEGIAIEEVSDPQPGPGEVRIRVEAAALNFLDT
                     LITRGKYQYKPELPFSPGAEVAGKIDIVGPEVGEFSPGQRVCAYLGWGGARELVVAHA
                     EHVIPVPDEVSTPVAAGINVTYGTALHGLMDRAKLKADETVAVLGASGGAGLAAVEVA
                     KRLGAKVIAVASSSEKLDICRQHGADQLLNYTTTDLKQGLRDLTGGRGADVIYDCVGG
                     DHAELALRSIAWLGRYCVVGFAAGAIPRIPLNLVLLKSCDIVGVFWGESARRNPRAHR
                     NNIETVLKWVAAGELRPHTHAALPLAETSEAIRMLDRREATGKVIVTM"
     gene            17926..18465
                     /locus_tag="VF226_05615"
     CDS             17926..18465
                     /locus_tag="VF226_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7073501.1"
                     /translation="MNDRVQSEFRGNWPPKPRGYFAIGAERMSKALNLGNLMRSAHAF
                     GASFTFTIGATYQALEARADTSKGQWHLPHYNWPTLDAMTLPQGCKLVGIELTDSAID
                     LPSFCHPLRAAYVLGPERGSLSDALLARCDFVVRIPTTFCINVAMAGAIVMYDRFRTM
                     AQFPERPVGLTETLRKTSS"
     gene            18539..19045
                     /locus_tag="VF226_05620"
     CDS             18539..19045
                     /locus_tag="VF226_05620"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX7073502.1"
                     /translation="MRGLVRPTAIAAILMATAVCASAQTKLIDSFTDWHFYSHENSGE
                     KLCFIATTAQSSEPAGMKRDPAYFYVSSWPKDGVRTELSVKIGYPFKEGSEVTVTVGS
                     ESYKLFTHQDRAFVSNPIEELKLLESMKRGTTMVVEGVSEQGTTTKDTFSLMGLTKAV
                     QTLTSQCG"
     gene            complement(19098..19640)
                     /locus_tag="VF226_05625"
     CDS             complement(19098..19640)
                     /locus_tag="VF226_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7073503.1"
                     /translation="MHPSAGHPESLPASEPIGPKLEDELKRAAVEPSSPREAQSQTGR
                     PKSPAKPPPLADFDRRRLKRIAGGRVEIEARLDLHGMRAGEAHSALRSFIFSACAKGQ
                     RHVLVITGKGGGAGLSDRPFDLDQPDRGVLKRSVPMWLAEPELRSVVVSFTQAHPRHG
                     GEGALYVQLRSRRRRPDHRE"
     gene            complement(19758..20861)
                     /locus_tag="VF226_05630"
     CDS             complement(19758..20861)
                     /locus_tag="VF226_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEX7073504.1"
                     /translation="MTEARVTFEAVSFSALPGWEEDEHSAAFGAFLKSGERLAKAADD
                     GSLPDLDRWARLLGIIRSALDLPGQANDARAFFETHFVPHRVVHARSHGLLTGYYEPV
                     LAGSRVPNGRYHVPVYRRPPDLVNLMDESMRGAAGKALTHARRTEDGRHLPYYTRSEI
                     ESGALSGRNLELLYLEDPVDAFFMHIQGSGLVTLPDGSHVRVSYDGKNGHPYTSIGRY
                     LVDSGLFRADEVSLETLKQWLRADPERGRQVMWQNASYIFFQELSVADAQSALGVMGI
                     PLTAGRSLAVDASVHLIGTPIYVVAPDLARAGERGTFRRLMIAQDVGSAITGPERGDI
                     YFGSGSEAGDQAGRTRHAGSFFVLLPRDAESKA"
     gene            complement(20975..21709)
                     /locus_tag="VF226_05635"
     CDS             complement(20975..21709)
                     /locus_tag="VF226_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEX7073505.1"
                     /translation="MDGRIDVITLLFLIVAVVVILKLRSVLGRRTGDEEARYERQLRA
                     QQEARERAAAAKGKVVALPRREREAATVGGHEEVAAADAETRVKNFAGGNTFLEQALL
                     HIARADASFDPEQFLRGAKQAYEMIVMAFAEGNRKLLKDLLSREVFDSFVAAISDRES
                     RGEQIDQSFVGINKAEIVEAELKGSTAQVTVKFVSQLISATRDSAGHVISGDPQRIRE
                     VTDIWTFAREVTSRNPNWRLIATQAP"
     gene            21933..22451
                     /locus_tag="VF226_05640"
     CDS             21933..22451
                     /locus_tag="VF226_05640"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HEX7073506.1"
                     /translation="MIRLTFLLLFVGYPLLELAVLIRVGQSIGVAATLAIVIGTAILG
                     LVTLKREGFAMMSRARQALAQGSPPIMPVAEGSLAFLAAVLLILPGLIGDTVGLLFLI
                     PPVRHAFAAWSLGRFASAGATRIRVFTTGRPHGGSGAGRRQSDPRKPGPVIEGEYTRV
                     DEDSDDRPPPRR"
     gene            22574..23092
                     /gene="secB"
                     /locus_tag="VF226_05645"
     CDS             22574..23092
                     /gene="secB"
                     /locus_tag="VF226_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEX7073507.1"
                     /translation="MADQPNNGASKPAGAAGDQPPFQARVLGQYIKDLSFENPNVGKL
                     VQEPGDNPNLQLEVNVNAKTVGKDVYESAIEFKASATNKAGVIYDLELVYAGLFKIEN
                     APPQALEPFLLINCPLLLFPFLRRLAADLTREGGFPPLLLDPIDFGSLYVRRKQQAQQ
                     QAQAAQTPSLKA"
     gene            complement(23106..23822)
                     /gene="dnaQ"
                     /locus_tag="VF226_05650"
     CDS             complement(23106..23822)
                     /gene="dnaQ"
                     /locus_tag="VF226_05650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEX7073508.1"
                     /translation="MREIVLDTETTGLDATQGDRLIEIGCLELVNRIPSGREFHRFIN
                     PEDREVHPDAEAVHGISNEFLKDKPPFSAIVDEFLEFVSDAALVIHNASFDMGFINME
                     LERAGRPPILMDRVVDTLALARRKHPAGPNSLDALCKRYGIDNSKRVKHGALLDSELL
                     AEVYIELLGERQATLVLGQDDVSARRSSIRRARAARPRPAPLPPRLTPEAEELHRAFI
                     ETLGQDAIWRRYMAAAQDKS"
     gene            complement(23866..24480)
                     /gene="coaE"
                     /locus_tag="VF226_05655"
     CDS             complement(23866..24480)
                     /gene="coaE"
                     /locus_tag="VF226_05655"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420274.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7073509.1"
                     /translation="MIVIGLTGSIGMGKSAVAERFRQLGVPVCDADALVHELYEGEAV
                     EPIEAAFPGTTSGGKVDRAKLGQAVLGDPRGLKRLESIVHPLVQAAEREFLRKAFEQG
                     ARMAVLEIPLLFETGGNARVDVTVVVSAPAEVQRQRVLQRPGMTPAKLEAILARQMPD
                     AEKRRRADFVVDTGGSIEESYAQVDAIVAALQNRGGRAYQQFWS"
     gene            complement(24510..25130)
                     /locus_tag="VF226_05660"
     CDS             complement(24510..25130)
                     /locus_tag="VF226_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HEX7073510.1"
                     /translation="MAESASFPQIILASASPTRQRLLTAAGIPFKAVAACVDETVIRE
                     TLTADGAEVDPADMAEVLARAKAEDVSARHPGSIVIGADQVLSCGSELFTKPGTMDRA
                     RDALLALRGRAHQLHTAAVIAEDGETEWVHVDTADLWMRNFSPAFVGRYLAKVGGDVL
                     GSVGAYQIEGPGIQLFERIEGDYFAILGLPMLPLLKELRARKAIPD"
     gene            complement(25123..25980)
                     /locus_tag="VF226_05665"
     CDS             complement(25123..25980)
                     /locus_tag="VF226_05665"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX7073511.1"
                     /translation="MPHRLPSYFHLHLVSDSTGETLTTIAKAASVQYAKVRPIEHVHP
                     LVRTARQLERVLQEISSAPGIVLYTIVNRELTERLEQRCRELAVPCLYVLEPIMKVFE
                     SYLGEPQTPIVAGQHVLDADYFRRIDALNFSMAHDDGHLPDDLNQADIIILGISRTSK
                     TPTSIYLAQRGYKTANVPIVSQLPLPEPLTRPHSAFVVCLVASVDRIAEIRRNRVRLM
                     SDRNLEDYVDREQIAQELAHSRRLCARHGWPVIDVTRRSVEETAVTILKLYQSRQAQN
                     TSQAEENDG"
     gene            26388..27449
                     /gene="hemE"
                     /locus_tag="VF226_05670"
     CDS             26388..27449
                     /gene="hemE"
                     /locus_tag="VF226_05670"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7073512.1"
                     /translation="MREIPQALTERAITCPFLMPFARLSPPSPPIWLMRQAGRYLPEY
                     RAVRQQAGGFLDLCYNPELASEVTLQPVRRFHFDAAILFSDILVVPHALGQHVTFAEG
                     EGPRLAPIRSREDIDRLDKLQVGFTFAPVHKTVLRTRAALPRDTALIGFCGAPWTVAT
                     YMVGGRGSPDQAEARLMAYREPETFAHLIDIVTEVSIKYLIGQIVAGADAVQIFDSWA
                     GSLPDDQFERWVIAPTRRIVSELKAHHQEIPVIGFPRAAGEKTARYVRETGVDGVSCD
                     TGMSLDAMAALADTEDVVVQGNLDPLLLVTGGEPLEARVRQILAAMQGRPFIFNLGHG
                     IVPQTSPENVARLVALVRS"
     gene            27465..27887
                     /gene="hemJ"
                     /locus_tag="VF226_05675"
     CDS             27465..27887
                     /gene="hemJ"
                     /locus_tag="VF226_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEX7073513.1"
                     /translation="MELWIKALHIISVIAWMAGMLYLPRLFVYHAASKPGSEQSETFK
                     VMERRLLRLIINPASIVVWITGPYLAWAWGMYMDGWLIAKFILVLALTALHHALAIWR
                     KQFAEDRNRHDQRFYRIVNEIPTVLLIGIVILVVVKPF"
     gene            28115..29380
                     /gene="rho"
                     /locus_tag="VF226_05680"
     CDS             28115..29380
                     /gene="rho"
                     /locus_tag="VF226_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX7073514.1"
                     /translation="MTELKLQDLKSKSPTELLSFAEEHGVENASTMRKQELMFAVLKQ
                     LAGKEIEITGTGVVEVLQDGFGFLRSPDANYLPGPDDIYVSPSQIRRFALRTGDTVEG
                     QIRSPKEGERYFALLKVNKINFEDPDKGRHKIHFDNLTPLYPTQWLKMEIEDPTIKDL
                     SARVIDIVAPMGKGQRALIVAQPRTGKTVLLQNIAHSITTNHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVISSTFDEPPTRHVQVSEMVIEKAKRLVEHKRDVVILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDSNALQRPKRFFGAARNVEEGGSLTIIATALIDTGSRMDEVI
                     FEEFKGTGNCEVVLDRKASDKRVYPAIDIARSGTRKEELLVPRDQLKKMYVLRRILNP
                     MGTIDAIEFLTDKLRQTKNNAEFFQSMNA"
     gene            29405..30142
                     /locus_tag="VF226_05685"
     CDS             29405..30142
                     /locus_tag="VF226_05685"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7073515.1"
                     /translation="MGDRVNAFGQPIGFPVPQWSPRPRPSRIVLDGRFCRLEPLDAER
                     HTADLYEANQADDGRGWTYMSHGPFASLADYRDWVEKASASEDPLFYAILEAGSGRAV
                     GVATLMRIDPPNGVIEVGNIKYSPALQRTPAATEAMFLLMRYVFDELGYRRYEWKCDS
                     LNQPSRAAALRLGFTFEGIFRQAVVYKGRNRDTAWFSIIDAEWPALKQAYERWLSPAN
                     FDADGRQQRRLSDLIAEARSAPSPSGR"
     gene            complement(30131..32179)
                     /locus_tag="VF226_05690"
     CDS             complement(30131..32179)
                     /locus_tag="VF226_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7073516.1"
                     /translation="MSENRLSRETSPYLLQHKDNPVHWWAWGPEALAEAKRTNKPILL
                     SIGYAACHWCHVMAHESFEDEATAALMNDLFINIKVDREERPDIDAIYMNALHELGEQ
                     GGWPLTMFLDPGGRPFWGGTYFPPQSRWGKPAFQHVLREVERIYREEPDKVRHNAELL
                     TDTLKARGAAAGKVQITDPLLEELTGRMVRAVDVVNGGLQGAPKFPQWSFFWLMWRGA
                     IRYDMPEAEAAVEKTLTHICQGGIYDHLGGGFARYSVDEHWLVPHFEKMLYDNALLID
                     LMTEVYRETGNPLFKQRIAETVSWLEREMISEGGGFAASLDADSEGEEGKFYVWSAAE
                     IADVLGPDDARFFNEIYGVAQEGNWEGHNILNRLGALELRSEADEARLAALRAKLFAR
                     RAERVRPGWDDKVLADWNGLMIAALAGAAFVFREPHWRDLAERAFAFIEDRMMSGGRL
                     VHSYRAGQAKAPATASDYANMTWAALRLAQATNSARYLDAAVRFVEQLDQHYWDARSG
                     GYFMTADDTPDVIVRMKSAHDDATPNANAVMLSNLVHLFMLKGETGYLERAEAILNAF
                     GGELQQNLIAHSALLAQTFDLLSPQHVIVLSGGDEAGAATLEKTVTSMSLPGALQHFV
                     EDAETVIAEADSLAGKRPVEGKATAYVCIGHTCSAPITDAEALAFLLHHKRKARSSPA
                     "
     gene            32248..33588
                     /gene="mnmE"
                     /locus_tag="VF226_05695"
     CDS             32248..33588
                     /gene="mnmE"
                     /locus_tag="VF226_05695"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7073517.1"
                     /translation="MIEVATIFALSSAPGRAGVAVIRISGPASGRVLDMLASPRPEPR
                     LAALRRLRNPSSGEVIDQALVLWFPAPGSFTGEDVAELHVHGGRAVVQAVLSAIGDVP
                     ECRPAEAGEFARRAFENGKLDLTMAEGLADLIDAETEAQRKQALRQAEGALADLYDGW
                     RDSLLEALALVEAAIDFSDEGDVGTTTFERAVPIVRDLETSIAHHLADGHRGEILREG
                     FQVVLAGPPNVGKSSLLNALARRDAAIVSEEAGTTRDVIEVRLDLEGLPVIISDTAGI
                     REATGVVEREGIRRTVARTRDADLVLWLMDATAPEPHLPPDLADMADRTLWVTNKADL
                     RTGGWPKTLPDDMLVVSALTGQGIDKLTRRIAAIARERIAPGEHPGLTLERHRLNLTE
                     CHQALVAFREGSPWQLELRAEDLRIAANALGRLTGRIDPEHVLDQIFGRFCIGK"
     gene            33805..35679
                     /gene="mnmG"
                     /locus_tag="VF226_05700"
     CDS             33805..35679
                     /gene="mnmG"
                     /locus_tag="VF226_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7073518.1"
                     /translation="MDTKTYDVIVVGGGHAGTEAAAAAARMGARTVLVTHKFATIGEM
                     SCNPAIGGLGKGHLVREIDALDGLMGRVADEAGIQFRLLNRSKGPAVQGPRAQADRSL
                     YRKAMQRAIRATENLEVIEGGVDDLLVRDGIVQGVALGDGRELRSGAVVLTTGTFLRG
                     LIHIGEVQIPAGRVGEAPAVKLADRLAALAFRLGRLKTGTPARLHRDSIDWDRLEVQP
                     GDDPPVPFSFLTERITTPQVVCHITTTTEATHAIIRANLDRSPMYTGQIGSIGPRYCP
                     SIEDKVVRFADRRAHNVFLEPEGLDDDTIYPNGVSTSLPEEVQIAFLRTIPGLESVVI
                     KRPGYAIEYDHVDPRELLPTLETKRLPGLFLAGQINGTTGYEEAAAQGIAAGINAALK
                     AGGSRRTFVVSRTEGYMGVMIDDLITRGVTEPYRMFTSRAEYRLKLRADNADQRLTPA
                     GVEIGCVGRHRKAAFKRKQQALAEGRRLLERLTLTPNEAARHGLDINHDGRRRSAFEL
                     LAYPEMTLERLAAIWPDIRTLEPKIASQLEVDARYASYVKRQAEDVETLRRDEAVAIP
                     LDFDFGTLPGLSNEVRQKLSLHRPATLAQAARIDGMTPAALLILLGRLKRSDRRRQSA
                     "
     gene            35676..36344
                     /gene="rsmG"
                     /locus_tag="VF226_05705"
     CDS             35676..36344
                     /gene="rsmG"
                     /locus_tag="VF226_05705"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX7073519.1"
                     /translation="MTRRSGIERIDEAGAFQRAFSVSRETVDRLERYEALLRQWQKAV
                     NLVAPSTLADVWHRHFADSAQLAALIPEAAESLVDLGSGGGFPGLVLAIVLMERRPYR
                     FTLIESDTRKAAFLREVARETRVPVDILASRIENPETQRKVGQVDVVTVRALAPLSRL
                     LGLAAPFFAPHTVGLFLKGRDIHGEIDEARAGWRFDVVLVPSKTDREGQIAVVRNVSA
                     KTEG"
     gene            36348..37178
                     /locus_tag="VF226_05710"
     CDS             36348..37178
                     /locus_tag="VF226_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7073520.1"
                     /translation="MTGKTVRSKGLRVIAIANQKGGVGKTTTAINLGTALAAAGERVL
                     VVDLDPQGNASTGLGVANQERIRTTYEVMLGQVRLLDAALQTQVPGLSIVPANSDLVG
                     IESQLMNEPTRPFRLRDAIGGLVGQQRADAEAAITYVLIDCPPSLNLLTLNAMVAAHA
                     VLVPVQCEFFALEGISQLKDNIEQIKATLNPGLEIQGVVLTMHDQRTALSREVAQNVR
                     AFFGAKVYDTMIPRNTRVAEAPSHGKPILLYDVNSAGSQAYMRLAAEIIERERHFKAA
                     "
     gene            37222..38094
                     /locus_tag="VF226_05715"
     CDS             37222..38094
                     /locus_tag="VF226_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7073521.1"
                     /translation="MATPLQKSRLGRGLASLIGDPALAEQKLPAEGEQRVVPIDQIRA
                     SKLNPRREFNEEDLAELAESIRQKGLVQPIVVRPDRLAGGYEIVAGERRWRAVQRIGM
                     HSVPCIVRELSDQEVLELGIIENVQRADLNPIEEAIGYRELIERFGYTQDQLAEVIGK
                     SRSHLANTIRLLKLPETVQSLVQEGKLTAGHARALVGREDAEALAQRIIERDMNVREV
                     EAMVHGAVTAAPSRRREKTADTLAFEKELSDVLGLKVEIKRGSGESGTLVIKYGNYDQ
                     LDYIRMRLVGSQGM"
     gene            38275..38616
                     /locus_tag="VF226_05720"
     CDS             38275..38616
                     /locus_tag="VF226_05720"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEX7073522.1"
                     /translation="MSHDVLARLAATIRQRRSEDAERSYTRQLIDGGPERCARKLGEE
                     ALETVIAGISQDERALANEAADLLYHLLVLLEARGVDWEEVIAVLEGRMGTSGLVEKA
                     SRAKQGEGGMS"
     gene            38613..39593
                     /gene="coaA"
                     /locus_tag="VF226_05725"
     CDS             38613..39593
                     /gene="coaA"
                     /locus_tag="VF226_05725"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEX7073523.1"
                     /translation="MTLALVRQDMPNKSAIQFSPYREFSREEWARLRADTPMTLVERD
                     LEQLSGLMQELSMEEVEQIYLPLSRLLNLYVAAAQELHAATFRFLGGPDMKVPYIIGV
                     AGSVAVGKSTTARVLRALLARWPDHPRVDLITTDGFLLPNRELEKRGLMNRKGFPESF
                     DTARLLNFLADVKSGCERVEAPVYSHFHYDVLPGQTVVVERPDILIVEGLNVLQPARL
                     PRDGKAIPFVSDFFDFSIYIDADAKVIEEWYVARFMKLRTTAFRDPAAYFHRYAHLSQ
                     EAAQARALEIWRTINLRNLEENILPTRQRASLILRKGSNHRIESVSLRRL"
     gene            complement(40772..41770)
                     /locus_tag="VF226_05730"
     CDS             complement(40772..41770)
                     /locus_tag="VF226_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX7073524.1"
                     /translation="MTNTRFDVVGIGNAIVDIIGRCTDDFLVRHGALKGHMRLVDAET
                     VDSLYRAMGPAVEVSGGSVANTMVGIASLGGRAAFIGKVAADEFGQIFAHDIRAAGVH
                     FDTAASSSGIPTARCLILVTPDGERTMNTYLGISPDLNGGEVDPEVIRSAGIVYLEGY
                     LFDRPEAKAAFRQAAQIAAQAGRKVALSLSDGFCVDRHRQEFLDLVKNSIDILIANED
                     EITSLYQVPTFEEAARLAQADTHLAVLTRSEKGSVICVQGQEPIAIPATPVNELVDAT
                     GAGDLYAAGFLFGLARGCSLEQAGRLGSMAAAEVISHFGARPQVKLSELARSNGLL"
BASE COUNT         7734 a        13362 c        13303 g         7581 t
ORIGIN      
        1 caggccgccg tcaaacaata gctcgagccc aatcttgtag aagtgaacgg cgtctccgag
       61 atccgtgacg atagctgacg cctctgcgta cgtcggcacg tccagcgcca cgatcagccg
      121 acgtgaaatg tcgtccctgg caatctcgcc gttgtgcatt gcgcgccgcc tcgtgtccgc
      181 gctgcctgga gtgccagcgc cctgtaacat gggttctacg gggctcacaa ggcggtcgag
      241 gggtcaaagc cctttcagaa ccttggcggc acgcgccaga cgttcgatct gcctcttaag
      301 cagctcctgc tgtgccttgt ctttcaagtg acccttctcg tcgaacgcat ctgcggcatg
      361 ctgcaccgag acctgctccg gcagaaccag cgcctgcaat ccgaccgcga ggacctgacg
      421 caaggtgatg aggccacgca cgccgccaaa acgccccggg ctcgcagacg agatggcgaa
      481 cacgcgtgtc ttgaacacct gcagcggcgg ctcgccctcg tcccgcacgc gggaaatcca
      541 gtcgagcgcg tttttcagca gcggtgtgat tgaagcgttg tattcgggcg ccgcgatgaa
      601 aatgccgtcg tgcacctcaa aaagcgcctt gagcttgcgc gcattgtccg gggcgccgtt
      661 cttgacctcg tgatcgcggt cgtagatcgg catgggatag tcgccgagat cggccaacgt
      721 ggcggcgagg ccgttggcat cggcgatctg cgcagcaagg cgggccagct gcttgttgtg
      781 cgagccctct cgggcagacc cggcaagaaa cagcagcttc ggcgtatgtg gcatgtgtcc
      841 agcctatggc gcaggttgct gctcaacaaa cattcggatt atccatgacc tgttttgcct
      901 gtcgagggga gtgatggagc tgacatcgga cgagcgcgcg attgccggcg gcgcacgcgg
      961 ggaggcttct gcgatggcca tgcgcattgt cgccgaaacc ggccggctgc tgggcgccga
     1021 ccggctgatc ccggtgcact cggctcatat cgatggctgc ctctaccatg gtgatgccgg
     1081 ggtgttgttc gccgagaagc tggttgagct tggcggccaa gttgccgttc ccgcgaccct
     1141 gaacgtcgga gcgcttgacc tgctgcaccc gaaacgcgtc cgtctggacg accggcgccg
     1201 cgacatggcg ctgcgtctga tgcgcgctta tgaggccctt ggctgcaagc cgacttggac
     1261 atgctcgccc taccaggccg ggcatcggcc gcccgcaggt gtggatgtcg cctggggcga
     1321 atccaatgcg gtggcgttct gcaactcggt gcttggcgcc cgaacgaacc gatacggcga
     1381 tttcctcgac atcgcctgcg ccatcgtcgg ccgggctccg cggtacggcc tgcatctgcc
     1441 ggagcaccgg cgagcgacaa tgctagtcga caccagcctg ctgtcagctg cgctccggaa
     1501 cctcgatgcg ttctggccgg tgctcggcgc gtggattggc cgcaatgccg gctccagcat
     1561 cgtggccata gacgggctgc agacctttgc ctctgaggac cggatgaagg cgctcggcgc
     1621 cgcagctgcc tcgttcggtg ctgtggggct gttccacgtc atcggcgtga caccggaagc
     1681 caagacccgg gccgatgctt tcagcgggca cgatccggag gtgaccgttc aactgacatc
     1741 tgagatggtg cgcatcgcac gcggctggct cagcacgagc agctcggatc aggtcgatgc
     1801 ggtcgcggtg gggagtcctc acctgtcgtt tgaagagatg atgacaattg cagatctcgt
     1861 cgacgggcgg cgcctgtcgc tgcccttcta tgctcacacc ggccggcacg tgctgaagca
     1921 gctcgaggag cggggaaagc ttgccgatat cgaagctgcc ggcgtgacgg tcgtcgttga
     1981 tacttgcatt gtggtgacgc ctattcttcc ggccaagtcc ggcgtcctcc tgacgaactc
     2041 cggcaaattc gccaactacg ccccaggaaa cacgggctgg tcggtcgtct acggcagcct
     2101 ctcggagtgc gtcgagacgg cagtccaggg tcggctcgtt cgcgacgagg cgctgtggtc
     2161 atgagcacgg agatgatagc cgggcaggtg ctctatcctg gatctgcgga aggtccattg
     2221 ctgaagctga ccgcccctct gagcttctgg ggcggcgtag atcctgcgac aggcgagatc
     2281 attctcggta gccacccaga gcgcggacgc agcgtgagtg gttccattct ggctcttgcc
     2341 gatccgatcg gatcttcctc ctcctcatca gtgctgctcg agctgatcca caatcgcgtg
     2401 gcccccgcgg ccattctgct cggaacacct gacgcaatcc tgatcgtcgg ctgtctggtc
     2461 gcgcgcgagc tgggactcaa ggcgccgccg atactagtgg ttgcgcgaga atggctacac
     2521 actctcccga acggccgatt gtccgtctcg gacgggatgg tccgccagat cagctagagc
     2581 ttttcctatt ggggctagaa acgccaacag caagcactcc gttagccggg aacggggaga
     2641 ttgggcgcga gcttgatttg gcgcttctgt ccaaatcgag aatttctcga aagcggtcct
     2701 tcagaccgcc cgcacctctt cttcgaccgg cactgcggtg cccatcgact cggtttcgtg
     2761 acctgcgagc gtctgcagct tgcccttgat gcgaaccatc atgtccagaa gctgctgccg
     2821 ctcctccatc gagaggtcgc tcaacgcgtc atccaccgtg ctttcggcca tcggcgcgat
     2881 cgaagcctgg acacgattgg cgcggggcgt gagataaatc cgcttaaccc gccggtcctt
     2941 cttgtcgggc cgccgctcga tccacttttt ggcttccagc cgatcgagca gccggcacgc
     3001 cgtggccttc tcgacttcga gcaggtcggc cacttcggac tggctgagac cgggacggcg
     3061 atggaggcgg gttacggcga gccactgtgc gcgcgtcagt ccgagctccc ggaccctgcg
     3121 atcgcatacc gtacgcaaaa gtcgcgcgac atccgcaatg atatagccga tataggcctc
     3181 atcctcgggt tccattgcgg ctggtccgga cactgttatt ctgatgtatt attactgatg
     3241 aagtgtcctt gcgaaaagca aaaagatttg ccaccggccc ggtcgatcag acattttgca
     3301 cagagtgcgg cgcccggatc gatcaggtct ggtcgagcct gaccggaata cccgcagccg
     3361 ccatatgtcg cttcgcctga cctatggtgt aggtcccaaa gtgaaaaatg gaggcggcga
     3421 gtactgcgct agcgtggccg agcagcacgc cttcgacgag atgatcgagc gtgccgacgc
     3481 cgcccgaggc gatgactggc acgctgaccg catcggccac cgcccgcgtc agcgccacat
     3541 cgaagccgct cttggtgcca tcgcggtcca tcgaggtgag caggatctct ccggcgccca
     3601 gagccgtgac ctcccgcgcg aacgccacgg cgtcaatgcc ggtcgggttg cgcccgccgt
     3661 gagtaaagat ctcccagcga tctgcttcac ccggctgtga gaccttcttg gcatcgatcg
     3721 ccaccacgat gcattgcgca ccgaacttct cggatgcctc gcgcacgaac tgcgggttct
     3781 tcaccgctgc ggtgttgatg gaaaccttgt cggctccggc ctcgagaagg cggcggatat
     3841 cctcgacgga gcgcaccccg ccgccgaccg tcagcggcat gaagcagcgc tctgccgtcc
     3901 tttgcacgat atcgagcaaa gtcccgcgat tctcgtggct ggccgtgatg tcgagaaagc
     3961 agagctcgtc ggctcccgcg gcatcgtagg cggcggcagc ctcgacggga tcaccggcgt
     4021 cgatcagatc gacgaactgg acgcctttga cgacgcggcc gtccttcaca tcgagacagg
     4081 ggatgatgcg gatcttgagc atagctgctt cttggcccgt atgcgccttc aatgcaatgt
     4141 catcttgcgt catcgtcgat gcgcgctccg aacgcaaatc taagggcggg ggatatggcc
     4201 aaggacacca gccacgatct gcagcacgtt gccgaggcgg tccgccggaa gcggctcgcg
     4261 gcagaggaca tcaagctgct tcgagagaag atcgcggcac tcgacgaggc cggcaccgag
     4321 caccgtttcg tcaaggcggc cctgcgcaat ctcaaggtgg cggggacgca gctcaaggac
     4381 attcaggagc gcaatcccgg cgagccgttc gacgagaagg ccttcgagca gctccccgag
     4441 catttgcgca agcgcatcat cacccggctc gacaacatcg ccaagcggat gtcgggcctg
     4501 gcggagcagg gcaagccggc ggagtacagc gatcccgcat tccgttgcct gcaagattac
     4561 gaaacctgtc gcaagcaggc gaaactctcg gtggtctggt gtcagctcgc gatggtggtg
     4621 tgcctgctgc gctcggcgct caccctttcg atccggcacg gcgcgaaatg agggcgagcg
     4681 ccgccttggc atcgagccgg ccatcgtaga gcgcccggcc cgtgatcgcg ccctcgagca
     4741 tcgcgtactc gggccgtagc agcgcttcga catcggcgag cgaggccagg ccgcctgagg
     4801 cgatcaccgg aatgcgtgtc gcccgcgcca gctctgcggt tgccggcagg ttcaggcctt
     4861 tcagcacgcc atcgcgctcg atgtccgtgt agatgatggc ggccacgccc gcgtcctcga
     4921 accggcgcgc cagctcgatg gcggtgagct cactcacctc tgcccagccc tccacggcca
     4981 ccttgccgcc cttggcatcg atcccgacag cgatgcggcc cggaaatcgg cggcaggctt
     5041 cgcgcacgag tgcgggatca cgtacggccg cggttccgag gatgacccgc cggaccccct
     5101 tgccgagcca catctccacc gtcgcgagat cgcgaatgcc cccgccgagc tgcaccggca
     5161 gggaaaccgc ggcgaggatc gcctccacgg cgcctgcatt cactggcttc ccggcgaacg
     5221 ccccattgag atccacgatg tgcagccatt gaaacccctg cgcctcgaac gtccgggcct
     5281 gcgcagcagg atcgtcattg aacacggtcg ccgcgtccat ctcgccgagc ttcaggcgga
     5341 cgcactggcc gtccttgagg tcgatggcgg ggaagaggat caatgctatg tctcaatgtg
     5401 agaagacagc gcgcggggag cccccgttcg agtcacacgt tcgctacgat cgcgggcgat
     5461 aaaggaatca aagccgtcgc gtcaagcact gttatgaatg ccgccgccag cgaaatcgaa
     5521 acagcacgcc gccccctaac cgaggccgac gccatcgatg tttggatcgc acgctggctg
     5581 cgcgcgcggc ggatcgacct ctgccgccgc taccagtgcg atccgcggcg cctttacgag
     5641 atctgggaag gacagcgctt ccctgcggcc cgcgtgaaag cgtggcgaat cttccaggag
     5701 cgttacccgg ggctgacgga tcgcatcgat cccggcccgc accggcggat cccccgcggc
     5761 cggcatccgg atcagcttac gctgttcgag tgagctgagc aggtgctcca gttccagaac
     5821 aaacgacgaa ctttttgctg gactcggatt ccgactcgcg gcatgttccc tttacgttct
     5881 gttaaggaat gccccggttc cgcggttctg gtcgcgcgta ccgcgtcttc ttcgccagga
     5941 gaccactatg cgcctctttc tcatctccta cgacctcgcc agatcgcgcc ggaacaagca
     6001 cgcgattgcg acagccatca tgaacctcgg ccaggcctgg gcacggccgc tcgagaccac
     6061 gtggtacgtc cgcgccaatg catcggaaca cgacattcag gtccggctgt ccgacatgct
     6121 ggatcctgag gacggactga tcgtgcaggc cgtgaacgag gaggcggtgc tggtcaacac
     6181 gtcgctccgc tggttccgtc agcggcgcca ggacgccgac cggccaagca atgtcattgc
     6241 tttcccggcg ggtctcatcg agcccctggt gcagccggag ctaccgctgg ctcgggcgag
     6301 ctagcctggt gcaggctcag ggccgccagc gcagaaaatt gccgatgagt tggaggccga
     6361 gagtctggct cttctctgga tggaactgcg ttccggccgt gttgcccttg gcgacgattg
     6421 ccgtcacagg gccaccgtag tccgtctcgg cgaccacgac gctgcgatcg cgggggacga
     6481 ggtgatagga gtggacgaag taggcgtgca gaccctgcgg tcccgtgcga atgccggcga
     6541 gtagcgggtg atcgttgatg accgtgagcg tgttccagcc catgtgcggg atcttcaggg
     6601 tcgggtccgc cggcgcgatg gctctgacgt cgccgtcgat ccagccaagg cccggtgttt
     6661 cgccgaactc gaggccccgc gttgccatga gctgcatgcc gacgcagatg ccgaggaatg
     6721 gccgggcacg ctcaacaacg gtctgggcga gcgcctcaat catcccgggc accccatcga
     6781 gcccccgctt gcagtcggca aaggctccaa cgcccggcaa cacgatccgg tccgccgctt
     6841 tgacgtcctc gggtcgggcg ctgacgttga tttcggccgg cacatcagct tcccgcgcgg
     6901 cccgttcgaa agccttgtgc gccgaatgca ggttacctga gccgtaatcg atgatgacga
     6961 cgcgttgcat ggtccggcgt ctcaagcctc cgaagcgacc ggcccggcat ccgctccggc
     7021 gatcgggcca ggcgcgtcgg ctgtcgcctg gcggggaggc gggccgatgc gcactgagcc
     7081 gtcatcgcgg tattcctcgc cgagccaagc ctggaagaat cggcgctcgc actcgatccg
     7141 agacgggccg ataacagcac ccatcatgcg ccagcgccgg cgctccagct tccaacggcg
     7201 gagcgatccc gcttcgaagc cgatggccag gtgaacggcg atgacggcca ccgttcgcca
     7261 ttgcgggctg acgtcaacgg caataagagc cacgctcagc gcgatcatca ggacgacgta
     7321 gaggaacagc gcgagccaca tccgatgcac cagcatccac agcggggcga acagcgcggc
     7381 catccaggag aagccgtcgc gcacgaagac catccgctcc gcgcgcttca cgcggtcttc
     7441 gggaggcttt ggcggctcgt aaacggtgta cgtctgcacc gattgtccac tcgctggtgc
     7501 tcagccggca agccggccct tggttgacgg aacgcgcccg gcctgccgag gatcctccgc
     7561 cacggccgcg cggagcgccc gcgccagccc cttgtagcag gtttcggcaa tgtggtggtt
     7621 attctctccg tagagggtcg cgatgtggag cgtgatccct gagttctggg caaatgcctg
     7681 gaaccattcg cgaaacagct ccgtgtccat gtcgcccagt ttcggccggg agaacgtgac
     7741 gttccagacg agatatggac ggcccgatac gtcgatcgcg acgcgggtca gcgcctcgtc
     7801 catcgccaga tgcacgtcgg cgtaccgggt aatgcccttc ttgtccccga gcgccatggc
     7861 caccgcttgc ccgatcgcga tgcccgtgtc ctcgaccgtg tgatggaaat cgacgttgag
     7921 gtcgcccttg gcatgaacct tcaaatccat cagcgaatgc cgagcgagct gctcgagcat
     7981 gtggtccagg aagccgatcc cggtcttgat gtcgaacgcg ccggagccgt cgagatcgat
     8041 agtaaccttt acttcggtct ccttcgtctt gcgctcaatg gtggcagtcc gggacacctt
     8101 gcgataaccc ctcgggttga ttgttcgaaa acaggcgttt atcagcttgc gcgccgccgg
     8161 ggtagtggtc attcgccggg ctggctccgc gcgctcttgc tttcggcgcc ccgcaaccca
     8221 acctagcgtg tgagttgacc atgcgtaatc cgccatcatc tttcgagtcc acttggcacg
     8281 gcaccacgat cctgacggtt cgcaagggcg gccgggtcgt gatcgccggc gacggccagg
     8341 tgagcctggg caacaccgtc atcaagtcga aggcgcgcaa ggtgcgatcc gtgctgcgcg
     8401 gcgacgtgat cggcgggttt gccggtgcaa cggccgatgc cttcaccctg ttcgagcgcc
     8461 ttgaagccaa gctggagcag tacccgggcc agctaatgcg ctccgcggtg gagcttgcga
     8521 aggactggcg gaccgaccgc tatctccgcc ggctggaagc catgatgatc gtcgccgatc
     8581 gtacgacgac actggtgctg accggaaccg gtgacgtgct ggaaccggag cacgacgtga
     8641 tgggtatcgg ctccgggggc atgtacgcac tcgccgcagc ccgcgccctc ctcgaccagc
     8701 cgctcgatgc cgaggcgatt gcgcgcaggg ccatggagat cgcggccgac atatgcgtct
     8761 tcaccaaccg gaacctcatc gtggagagtc tgccgagcga caggtaggac cgggaccgcg
     8821 caatgggggc gcgccggctt gagaagactt gggaacggat catatgctcg cttggggata
     8881 tttgctaact gccgggattt gcgaggtcct ggccacgacg gccttccgct acgcagaggg
     8941 atttaccagg ctcgggccca cgctcgcttt cgccggctct gctgtcgcca gtctgctgct
     9001 gctccagctc tccctttccg gcattccgct tggtacggcg tatgcggtat ggacgggcat
     9061 cggtgccgcc gggaccgcca ttctcggcgc gatgtatttc aatgagccga ccacgaccat
     9121 gcgcctgttc ttcctgacgc tgctgatcgg ctccattctg ggcttgaagc tggcgtcttc
     9181 caactaggca gagatgaatg acgacctttt caccccgtga gatcgtttcc gagctggatc
     9241 gctacatcgt cggtcagaac gatgcgaagc gagccgttgc catcgcgctg cgcaatcgct
     9301 ggcgccggca gcagctcagc gaagatctgc gcgaggaagt tcaaccgaag aacatcctga
     9361 tgatcggtcc aacgggcatc ggcaagacgg agatctcacg ccggctcgcc aagctcgcca
     9421 atgcgccgtt catcaaggtc gaagcgacca agttcaccga ggtcggctac gtcggccgcg
     9481 acgtcgaatc catcatccgc gatctcgtcg aggtcggcct ggcgctcgtg cgcgagacga
     9541 agcggcgcga ggtgcgggcc cgcgcggaga agatggccga agagcgcgtg ctcgatgcgc
     9601 ttgtcggaga gcatgcttca gcagccaccc gcgaggcctt tcgcaggcgc ctccgggcga
     9661 acgagctcaa cgacaaggaa atcgagatca aggtgacgga tactggcggc ggcattccca
     9721 tgtttgagct gccgggtgtg ccgggtggat ccatcggcgc catcaacctc ggtgacatgc
     9781 tgggcaaggc gttcggcggc cggcagaaaa cccgccgtgt caccgtcaag gacagctacg
     9841 agatcctgat cgctgaagag agcgacaagc tcctcgacca ggagcagatc acgcaggagg
     9901 ccatccgcgc cgtcgagaac aacggcatcg tcttcctcga cgagatcgac aagatctgct
     9961 cgcgcagcga tggcggccgc atcggtgccg acgtttcccg cgagggcgtg caacgcgatc
    10021 tgctgccgct catcgaaggc accaccgtgt cgaccaagta cgggccggtg aagaccgacc
    10081 acgtgctgtt catcgcctcg ggcgccttcc acgtcgcgaa gccttccgat ctgctcccgg
    10141 aactgcaggg gcgtctgccg atccgcgtgg aattgaagcc tctgacgcgc gaggacttcc
    10201 gccgcatcct cacggagccg caggccagcc tcatcaagca gtacatcgcg ctgatggcaa
    10261 ccgaaggcct caccctcgag ttcactgacg atgccatcga cgccatcgcg gatacggcag
    10321 cccaggtgaa cgcgacggtc gagaacatcg gtgcacggcg tcttcagacc gtgatggaac
    10381 gagtgctcga tgacatctcc ttcgatgcct cggaccgcgc cgggcaaacc atcaccgtcg
    10441 atgcacagta cgtcagggat cgcgtcggag acctcgcgaa gaacaccgat ctgagcaagt
    10501 tcattctcta gcccttcgcg gaagatcggc cgcaaggtgt gatgcgagga ggcgagccat
    10561 gccgcggctt ttcacaggaa ttgagcttcc ggacgacgtg cgtgaggcac ttgcccgcct
    10621 gaggcagccc cttcctggtg cgcgctggat cgagcccgag aactatcaca tcacactgcg
    10681 cttcgccggc gacgtggaca accgcctcgc cgccgagttc gcccacgcat tgagcgaagt
    10741 cgaggttcag gccttcgagg tgcggtttgc gggcacaggc gcgttcggcg gcaatgaacc
    10801 gcgtacgctc tgggcgcgca tagagccgac accagagctg gaagcgctgg cccgcgccca
    10861 tgagcgcgcg gcccgtgcgg caggtctcca gccggagacg cgaaaattca agccgcacgt
    10921 caccatcgcc cggctgcgca acaccagtgt cgatgtcgtc gctcgctatc tccaacgcca
    10981 gggcggattc cggacagagc cgttcctcgt cagccgcttc gcgctgttct cctccaagcc
    11041 ccaggtcggc ggtggcccgt atgtaatcga ggagacctat ccgctcatcg ggggtgacta
    11101 cgccgagtat gaggacgagt actgaaggct gacgtccgaa cgagctgtgc tttgggagga
    11161 acggccatgg ctcagaagct ctcacccgaa gcccgcgcag aggcgttggc gcggctgtca
    11221 ggctggcaac ccgtcgaggg ccgggatgcc attttccgcc agttcaagtt cgccgatttc
    11281 aatgccgcct ttggattcat gacacgcgtg gccctggtgg ccgagaagat gaaccaccac
    11341 cccgaatgga cgaatgtctg gaaccgcgtc gacgtcacgc tgacaagcca cgatgcgggc
    11401 ggcctctccg cgcgtgacat caagctcgcc gaggccatgg atcggattgc gggcacgcag
    11461 gcctgacctg ccgatccatg ccgggcttcg cccgttctcc gaaagcttcg gcgaactttt
    11521 ctagcgcggt gccaagcgga tcgcgccatc gaggcggatg ctcgcgccgt tcagcatcac
    11581 gttctcgcaa atgtggatcg ccagcgctgc gtactcggac ggatcgccga ggcgcgaggg
    11641 gaagggtacc gttgccgcca gggacttctg cgcctcctcc ggcaggccgg cgaacatggg
    11701 cgtccggaac aggcccggca ggatggagca gacgcgaacg ccatcgcgag aaaggtcacg
    11761 cgcgacgggc agcgtcatcg aggcgactcc gcccttcgat gccgcatacg cgacctgtcc
    11821 gatttgcccg tcctgcgctg cgaccgaagc cgtattgatg atgaccccgc gctcgccgtc
    11881 cgggccaaga ggatcgagcg tcagcatgcc cgcggcgcat tttgaaatca tcagaaacgt
    11941 gccgaccagg ttgacctgga tcaccttggc gaagctcgca agatcatgag gttcgatggc
    12001 gcctgtatcc ttgacgcgcc ggacgacgcg cttggcaatg gcaatgccgg cgcaattgac
    12061 caggatgcgt tcctgcccat gggctttgcg gacggcagcc aaggcggctt cgacgctttc
    12121 ctcgttcgtc acgtcgcatg gtgcgaaggt gcccccgata cgctgtgcgt gctcacgccc
    12181 ggcggcttcg ttgagatcga gcaggctgac cttgacgccc ttctcggcca atgccgctgc
    12241 cgtcgcggcc ccgaggccgg acgcaccacc cgtcacaacg gctgcaatgt cgcttccgag
    12301 cttcatatcc cgtgtcctcc ttacgccggc ccaatggcgt atgtcgattg aatggggttg
    12361 tctcttctct taggtttcgg cggctcgccc ttaaagcgct gcttgcctga aggagacctc
    12421 cgtggcccac aacggtctga tcgatcttta tagcgataca tccacgcgcc cgacaccggg
    12481 gatgcgccaa gccatggcca atgccgatgt gggcgacgag cagcgcggag aagatccgac
    12541 caccacggcg ctcgaggagc gcatcgccgc gcttttgggc aaggaagccg cggtgttcct
    12601 gccctcgggc accatgtgca atcagatcgc gctcctggtg cactgccggc ccggtgatga
    12661 agtcatcggt gccgccggca cgcacgtctt cacgtccgag gcgggcggcg gctcggcgct
    12721 ggccggcgcg cagacctggc ccatcgtttg cgacaggggc atattcgacg gctccgacct
    12781 cggcgctgcc attcgcgagc cgagtcgcca ctcaccgcgt tcacggctgg tcgtcgtcga
    12841 gcagaccgta aaccgtggcg gcggcgcgat ctggcccgtc gagaagatcg aagctgtcgc
    12901 cgagctcgcg catcgacgcg gtctcgccgt tcacatggac ggtgcccgtc tgatgaacgc
    12961 cgttgtcgcc accggcattc cggctcgcgt catggcggcc cgatgcgaca gcgtgtggct
    13021 cgacctgtcg aaagggctcg gatgtccggt cggcggcgtg ctggccggct cgcgcgactt
    13081 catcgacgag gcctggcgct ggaagcagcg gatcggcggc gccatgcgcc agtcgggcat
    13141 tctggcggcc gcggggctct atgcgcttga ccaccacgtc gaacggctgg cggatgatca
    13201 cgccaatgcg gcgctgctca gcgagcgtat ccgagaaatc cccggagtca ggctcatggc
    13261 cccaagaccc gagaccaaca tcgtctattt cgacacgagc ggtacgggcc tgacggccaa
    13321 ggcggtgacg gaagcgctgc tcgcggagaa cgtgcggatg gggacgtcct acggtggcat
    13381 gatccgggcc gtcacgcacc tcgacgtgtc gcgtgacgac gttgaggccg cggcggccgt
    13441 actccgccgc gtgctggatc gccgatagac agagcccgaa ccagtcaggc tgtcctcagc
    13501 cgttggagca cgacagccag cgcaccgaac gccaagccga aaggcacgct ccaggcgaca
    13561 tggaatccgg gaatatcgga cgtgagctgc aagccagtga gcggcgtgaa tatcgcggca
    13621 tcgagcacga cgggaaacac cagcaacaga ccggcggccc agaacagtgc cgtctcgagc
    13681 agcgtattgc gcttcaccat ccatctttgc agcgcaaagg cgagcgcaac gataccggcg
    13741 gccacgacca gcgtgatttc caccacgtcc acccagctcc gtccttccgg catatgcagc
    13801 aagaggccga caccgtgtgg atctacgacg aacacgaacg gcacgaggaa ggccggcatc
    13861 gtgtatttcc aggactgcag cgtcgtgatg tacggattgc cgcccgtgat cgccgccgcg
    13921 gcgaacggtg acagggctgt tggtggcgac acctcggaaa gcacggcgta atagaagatg
    13981 aacatgtgcg ccgcgaaatc cggaacaccg agcttgatga gcgccggggc cgcgatgacc
    14041 gcgcacatga tgtaagaggc ggtcaccggc acagcgagcc cgacgatcca cacgatggcc
    14101 gccgtgaaaa tcgtggtgag cagcagactg ccctgcgcgt agccgatgat gatatcggca
    14161 aaccgctgcg cgaggcccgt caaagtcacc acgccgacga tgattcccgc cgtcgcgcag
    14221 atcgaggcca cgctgaggac gccggtcgtg ccgtcgcgca gcgcctgcgc caggcgggct
    14281 gccaccggtg cgatctgcgg caggaacagg cctgccgcgg cagtgagcgc cagtgcgatg
    14341 aagacggtca gcgaataggc attgccgtct gcaaacccga tcgcggagag caccgctgcg
    14401 acggcaatcg tcgcgccgac gacgatgcgc ggaacaagcg ccgtgtccgg ccggatcgcg
    14461 ctggagccga tggccagcat ggttgcccag aacaccgcca gcacgggcga aaagccgagc
    14521 accaagaagg cgatgatcga gaccagcgag ccaaagtggt accagtagcc ggcaagcagc
    14581 cgccagagcg tccctttctc gaccatcaga tcgctggtgc cgccagtctt tctggcgtcg
    14641 acctcgacca tggtccagat cgccagatag tagagcagcg tcgggatggt cgccatcaaa
    14701 atcacgtcga agtaggagat cttgagaaac tcggcgatga gaaaggctgc cgcgccaagg
    14761 accggcggcg agatgatggc gccaagtccg ccggcggcca gcagtccgcc ggcggcgttc
    14821 ttttcgtacc cggctttggc cagcagcgga taggccaccg tgccgatcgt gaccgtcgtc
    14881 gccacgccgg accccgacgg gccaccgagc aggaaggatg acagcacgac ggcgcggccg
    14941 gccgagttcc gtttgccacc catagcggcg aacgagaaat cgatgaagaa cttgcccgcg
    15001 cctgaaactt gcagaacggc cccgaagatc gtgaacagga tgatgaggct cgaggagacg
    15061 tccacagtcg ttccgaaaat gccttcgagc gtcatgtaga ggtgcccgac gaggcgatcg
    15121 agcggatagc cgcgatgggt ccacggttca cccaggtggt tgccgaacaa cgcatagagg
    15181 atgaagacga tcgccaaggc gggcatgata aaacctgtcg cccgccgcgt tgcttccagc
    15241 aaaagcacaa tgaagataat gccgacaaca acgtccatcg gctccggcat gattgcgcga
    15301 tcgccgaact cctcaccctg catcaggacg tagacgatga cgcccaggct cgcgaaagcg
    15361 agcagccagt cccaccaccg gatgcgatcg cgcaggctcg gcaccgccgg gaacagaaga
    15421 aacacgagga acaggacgca agcgacgtgg acgggccgca ggacataggc agggacgatg
    15481 tcgtaggcgg catagatatg gaacagcgac acgccgaccg cgatcgtcgt aatcgccttt
    15541 ccgacgaggc caccgagccg attgagcgcg ccttcctctt cctcgatgta gtgctcgatg
    15601 atcttgcgat cgggctccgg cgtctcgact gcggccggag tgttctccgc ctctccggcc
    15661 gcagatgcac catcgctggt cctcgtcaaa gtcagtctcc tttgggctcg cgcattcgcg
    15721 aatcttggcg gctaaaacgg gtcaccgggc gcgaggggcc cggtgaccgc aaatcgagcg
    15781 acaacgccag actcgttcag tcgaccttga tgcccttctc gttgaagtac ttgagtgcgc
    15841 ccggatggaa cggaatgccg gaccgcttca ccgactgcag ctccggcttg atgttcaacg
    15901 cctccttgtg gaccttcgcg agctcctcac gcttctcaaa ggtgatcttc gttatgttgt
    15961 aggcgaggtc ctccggcatc gcgtcgttca cggccaggat gttccagacg gtgatgttat
    16021 tcgcatcctg atccatcccc gagtaggtct ccttggggat gacggcctcg gcatagagcg
    16081 ggccgtactt ctcgttcatc tttggcgtaa ggtgcgcgat ctcgaggaac ttgatcttcg
    16141 tgttcggcgt cgcggcgagg tccgtcaccg ccgccgtggg cagaccaccg acccaaaaga
    16201 aggcctggat cttgccgtcc ttgatggcat tgacgctttc ggcaaccccg agacgctcct
    16261 tcccgatgtc cttctcggga tcgatgccgg ccgcttccag cacacggaag gcaaacacct
    16321 cagttgcgct tcccggtgag ccggtggaaa cccgcttacc cttgagatcc tcgatctttt
    16381 cgatgccagt gccgtccacg gtcacgacgt gcatgtggtt cggatagagg acggcgagcg
    16441 ccttcacggg cagctttttc gggaacttgc cctgaccgtt gatcgcatcc caggccgcat
    16501 cgacctgcga gaaggcgatg tccgtcctgc ccgagccaac gagcttcatg ttgtcgacgg
    16561 agccgcccgt gacttccgcc gtggccgtta cacctttgag ctccttgctg aggatgttcc
    16621 ccagaccgcc acccagcggg taatagacgc cgccggtgcc gccggttgcg atcgtcagct
    16681 gcttgtcctg ggcgttggca ggaccggtcg cgaatacacc gaggcccatc gccaccaggg
    16741 ccatcaaaaa ttgctttttc atcggatcga gcttctcctt gcgtgaatcc cctgagccag
    16801 cgcggggctc agcacaccgc tgttcgttgt gaattgcgca tggcttcctg caagcgggct
    16861 acatggtgac gatcaccttg cccgtggctt ccctccggtc gagcattctg atcgcctcgg
    16921 atgtctccgc aagcggaagc gcggcgtgag tgtgcggcct cagctctccc gctgcaaccc
    16981 atttcagcac ggtctcaatg ttgttccgat gcgcacgcgg atttcgccgc gcggattcac
    17041 cccagaacac cccgacgatg tcgcagctct tgagcagcac gagattgaga gggatacgcg
    17101 ggatcgcgcc ggccgcgaat ccgacaacgc agtatcggcc caaccaggcg atggagcgga
    17161 gagccagctc ggcatgatcg ccgccgacgc agtcgtagat cacgtctgcg ccgcgcccgc
    17221 cggtcagatc gcgcaagccc tgtttcaggt cagtcgtggt gtagttcagc aactggtccg
    17281 cgccgtgctg gcggcagatg tcgagctttt cggatgagga cgcgacggca atgactttgg
    17341 ccccgagccg ctttgccacc tcgactgccg cgagaccagc accgccggac gcaccgagga
    17401 ctgcaaccgt ttcgtccgcc ttcagcttcg cccggtccat caggccatgc agagccgttc
    17461 cataggtcac attgatgccc gcagcgacgg gcgtgctgac ctcatccggt acaggaatga
    17521 cgtgctcggc atgcgcgacg accagctccc gcgcgccacc ccagccgaga taggcgcaca
    17581 cccgttggcc cggtgagaac tctcccactt ccggaccgac gatgtcgatt ttgccggcaa
    17641 cttcggctcc tggcgagaac ggcagctccg gcttatattg atacttgccg cgggtgatga
    17701 gcgtgtcgag aaagttgagc gccgccgctt ctacgcggat gcggacctcg ccaggaccag
    17761 gctgtggatc agaaacctcc tcgatggcaa tcccttccgg accggccagg gtcttgcaca
    17821 acgccgctct catgtgacac ctcctccatg tcagccgatg gatggctgcc cggagcgtaa
    17881 cggctaccgc cggactgagc caagccggca acggcaccga gtatcatgaa cgatagagtc
    17941 cagtcggaat ttcgcgggaa ttggccgccg aagccacgcg gctacttcgc catcggcgcc
    18001 gagcggatgt cgaaggcgct taacctgggc aacctcatgc ggtcggccca tgccttcgga
    18061 gcgagcttca cgttcaccat cggcgcgacc tatcaggccc tggaggcacg cgccgacacg
    18121 tcaaaggggc aatggcacct gccgcactac aattggccga ccctcgatgc gatgacgctt
    18181 ccgcaaggct gcaagttggt aggcatcgag ctcaccgatt ctgccataga tttgccgagc
    18241 ttctgtcatc ctttgcgcgc agcgtacgtg ctcggcccgg agcggggctc gctctcggat
    18301 gcactgctgg cgcggtgcga cttcgtcgtt aggatcccga cgacgttttg catcaatgtc
    18361 gccatggccg gggccatcgt catgtacgac cgcttcagga ccatggcgca attcccggaa
    18421 cgaccggtcg gtctcacgga aacgttacgc aaaacatcat cgtgacaggc cctgcagcaa
    18481 atcactatag tttcggaaga tctccaatct ctgctgccgt cccaaatcgc gaggtgcaat
    18541 gcgaggacta gttcgcccaa cagctattgc tgccatcctg atggcgacag ccgtttgcgc
    18601 gagcgcgcag acgaagctca tcgactcctt caccgattgg catttctatt cgcatgaaaa
    18661 ttcgggcgag aagctctgct tcatcgcgac cacggcgcag tccagcgaac cggcgggtat
    18721 gaaacgcgat cccgcatact tctatgtttc ctcctggccg aaggatggtg tgcgcaccga
    18781 attgagcgtc aagatcggtt atcccttcaa ggagggctcc gaggtgaccg tcacggtcgg
    18841 gagcgagagc tacaagctct tcacgcacca ggaccgcgcc ttcgtgagca atccaatcga
    18901 ggagctgaag ctgctcgagt cgatgaagcg cggcacgacg atggtcgtgg agggcgtgtc
    18961 ggagcagggg acgacgacca aagatacgtt ttctctgatg ggcctcacga aggcggtgca
    19021 gacgctgacc agccaatgcg gatagctcgc aacgcggaat tttgcccgag cccgcagccg
    19081 aggttcatcg caaaagctca ttcgcgatgg tcaggccgcc ttcggcggct acggagctgt
    19141 acatagaggg cgccctctcc tccatgtcgg ggatgcgcct gtgtgaagct gacgaccact
    19201 gagcgcaact ccggctccgc gagccacatg ggcacgctgc gcttcagaac tccgcggtcg
    19261 ggctggtcca agtcgaaggg acgatcgctc aagcccgcgc caccaccctt tcccgtaatg
    19321 acgagcacgt ggcgttgccc tttggcacac gcggagaaga tgaagctcct cagtgcactg
    19381 tgcgcttcac cggcgcgcat accatgcagg tcgagacggg cttcgatttc gacccggcca
    19441 cccgcaatac gcttcagtct tcgccggtcg aagtccgcaa ggggtggcgg cttggcgggg
    19501 cttttggggc ggccggtctg agactgagct tcgcggggac tggagggttc caccgccgcc
    19561 cttttcagct cgtcctccag cttcggtcca attggctccg atgccgggag cgattcagga
    19621 tgcccggcag aaggatgcac tcgcgccttc cttctgaagg gcttcaggct acgagccaca
    19681 gatgaccaaa gcgcctcgtc ctccggcgtc aggaggcgct gctgggagcg gggaggtcgc
    19741 cctggccgac tcatggccta agctttggac tccgcatccc gcggcaacag cacgaagaag
    19801 ctgccggcgt gccgcgtcct gcctgcctga tctccggcct cgctgccgga tccgaaatag
    19861 atatcgcccc gctccggacc cgtgatcgct gagccaacgt cctgagcaat catcaagcgt
    19921 cggaaggtac cacgctcgcc agcacgggcg aggtcgggcg ccaccacgta gatcggtgtt
    19981 ccaatgagat gcacgctcgc atcgactgcc aggcttcggc cagcagtaag cgggataccc
    20041 atgacgccaa gcgccgattg ggcgtcggcc acggacagct cttggaagaa gatgtaggat
    20101 gcattctgcc acatcacctg tcggccgcgc tccgggtccg cgcgaagcca ctgcttcagg
    20161 gtctcgagcg agacttcatc cgccctgaag aggccggagt ccacgagata tcgcccgatc
    20221 gacgtgtagg ggtgtccgtt cttcccgtca tagctcacgc ggacatgcga cccgtcgggc
    20281 aatgtgacaa ggcccgaacc ctggatgtgc atgaagaacg catcgacagg atcttccagg
    20341 tagaggagct ccaagttgcg gcccgacaat gctccggact cgatctcgga gcgcgtatag
    20401 tacggcaagt gccggccatc ctctgtacgg cgcgcatgcg tcaaggcctt gcccgcagcg
    20461 ccacgcatcg attcatccat caggttcacc aggtccggcg gccgtcggta gaccggaacg
    20521 tgataccggc cattaggcac acgggagcca gccaggaccg gctcatagta gccagtcagg
    20581 agcccgtggc tgcgtgcgtg aaccactcga tgcggaacga agtgcgtttc aaagaatgcc
    20641 cgggcatcat tcgcctgacc cggcagatcc agtgctgatc ggattatccc gaggagacgc
    20701 gcccatctgt cgagatcagg aagagagccg tcatccgcgg ccttggccag ccgttcgccc
    20761 gacttcagga aggccccgaa agccgcagag tgttcgtctt cctcccaccc gggaagggca
    20821 gagaacgaga cggcctcgaa tgtcacacgg gcttcggtca cgacacctcc tgcactagag
    20881 cgcaggcggc gcaacgcaga cgcggtcctc gacaggagat tcgctcagac aagcccacgc
    20941 ggccgcacat gtccgctccc gagcccctgg ctcgtcacgg cgcctgtgtg gcaatgagtc
    21001 gccagttcgg gtttcgcgac gtcacctcgc gcgcgaaagt ccaaatatca gtgacctcgc
    21061 ggatgcgctg gggatcaccg gagatgacat gtcctgccga atcgcgcgtc gccgatatga
    21121 gctggctgac gaacttcacc gtgacttgcg cggtcgatcc tttcagctcc gcctcgacga
    21181 tctccgcctt gttgatgccg acgaagctct gatcgatctg ctcgccgcgg ctctcacgat
    21241 cggagatggc agcaacgaag ctgtcgaaga cctcgcggct gagaagatcc ttcaggagct
    21301 tgcggttgcc ctcggcgaag gccatgacga tcatttcgta ggcctgcttg gcgccgcgca
    21361 ggaactgctc ggggtcgaaa ctcgcatccg ctcgcgctat atgcaggagc gcctgctcca
    21421 ggaacgtgtt tccaccggcg aagtttttca cgcgtgtttc cgcatcggct gctgcgactt
    21481 cttcgtgacc gcccaccgtg gcggcttcac gttcccggcg ggggagggcc accaccttgc
    21541 ccttggccgc ggcggcgcgc tctcttgcct cctgctgcgc ccgaagctgg cgctcgtaac
    21601 gggcttcctc gtcgcccgtt cgccgcccaa gtacgctacg cagcttgaga ataacgacga
    21661 ccgcgacgat caggaacagg agggtaatga cgtcgattct gccatccatc tgaagctgct
    21721 ttcgtggcga ggaccagtgt tgacttgatg tatgatgaag tcaggacagg gtccagggcc
    21781 cgggccggtg aaaacgtcgc gcggttgagc gcaacgcatg gtccttcgcg accctaactt
    21841 agggcattgt ctcgcccaag ccaatcggaa tgacttggcc gcacgccccg ccggccaaag
    21901 cagcactcga gcggggccct ttcggtaggt tgatgatacg tttgacattt ctgctgttgt
    21961 tcgtgggcta tcccctgctg gagctcgcgg tcctgatccg cgttggccag tccatcggcg
    22021 tggccgcgac tctcgcaatc gtgatcggga cggccattct cggcctggtc acgctcaagc
    22081 gcgaaggctt cgcgatgatg agccgcgctc gtcaggcgct cgcacagggc agcccgccga
    22141 tcatgcccgt cgccgaaggg agtctcgcct tcctcgctgc cgtcctcctc attctgccag
    22201 gtctgatcgg cgatacggtg gggctgttgt ttttgatccc tcccgtgcgt cacgctttcg
    22261 cggcatggag cctcggccgg ttcgcatccg ccggtgcaac ccgtattcgc gttttcacca
    22321 ccggacggcc gcacggcggc tccggagcag gccgtcgaca atcggaccct cgcaagccgg
    22381 gtcctgtgat cgagggggag tacacgcgcg tcgacgagga cagcgatgat cggcccccgc
    22441 cgcggcgctg aggcctgaag acagctaacg gttcacgaac aaagagctgg cattgccgcc
    22501 cggcctcgag cgtgatagcc agcgcctcgg tacctgaatg cgcttccgac gagccggaat
    22561 ggaggacgac gccatggcag atcaaccgaa caacggcgcg agcaaacctg ctggagccgc
    22621 tggcgatcag ccgcccttcc aggcccgtgt tctcgggcaa tacatcaagg atctctcgtt
    22681 cgagaaccct aacgtcggca agctggtcca agagcccggt gacaatccga acctgcagct
    22741 cgaagtcaac gtcaacgcga agaccgtcgg caaggacgtc tacgaaagcg ccatcgagtt
    22801 taaggcgagc gcaaccaaca aggccggggt gatctacgac ctcgagctcg tctatgcagg
    22861 gttgttcaag atcgagaacg caccaccgca ggcgctcgag cccttcctgc tcatcaattg
    22921 cccgctgctg ctgttcccgt tcctgcgccg cctcgccgcc gacctcacac gagagggtgg
    22981 ttttccgcca ctgctgctcg acccgatcga tttcggcagc ctctatgtcc ggcgcaaaca
    23041 gcaggcccag cagcaggcgc aggcggcaca gactccctcg ctcaaggcgt gaagagcgcc
    23101 gctcgtcagc tcttgtcctg tgcggcagcc atgtaacggc gccaaatggc gtcctgaccc
    23161 aatgtctcga tgaaggcccg atgcaattcc tcagcttcgg gcgtcagccg cggcggcaac
    23221 ggggcaggac gcggccgagc ggcccgggcc cggcggatgc tggagcgcct tgccgaaacg
    23281 tcgtcttggc ccaggaccag cgtcgcttgc cgctcaccca gaagctcgat gtagacttcc
    23341 gccaggagct cggagtccaa cagagcgccg tgcttgacgc gcttcgagtt gtcgatccca
    23401 tagcgcttgc agagcgcgtc gaggctgttc gggccagccg ggtgctttcg ccgcgccaac
    23461 gcgagcgtgt cgaccacgcg atccatcaga atcggcggtc ggccggcgcg ttcgagctcc
    23521 atgttgatga agcccatatc gaagctcgcg ttatgaatga cgagcgccgc atcgctgacg
    23581 aattccagga attcgtcgac gatggctgaa aagggaggtt tgtccttcag aaactcgttg
    23641 gaaattccgt gaacggcttc tgcatcggga tggacctcgc ggtcttccgg attgatgaac
    23701 cggtgaaact ctcgtccgga agggatgcgg ttcaccagct ccaggcaccc aatttcgatc
    23761 agtcgatccc cctgagttgc gtcgagcccc gtcgtctccg tatcgagaac gatctctcgc
    23821 agcatccccc cggccccccg gcctcagtcc ctcgtcaccc ctccgtcagc tccagaactg
    23881 ctggtaggcc ctgcctccgc gattctgcag cgcggcgacg atagcatcga cctgggcgta
    23941 gctctcctca atggagcccc ccgtatccac aacgaaatcg gcgcggcggc gcttttccgc
    24001 atcgggcatc tggcgggcga ggatggcttc gagcttcgca ggggtcatac cgggccgctg
    24061 caggacccgc tgccgctgga cttcggcggg agcgctcacc acgaccgtca catcgacccg
    24121 cgcattgccg ccggtctcga acagaagcgg aatttcgagc acggccattc tggcgccctg
    24181 ctcgaaggcc ttgcgaagga attcgcgctc ggcggcctgc accagcgggt gcacgatcga
    24241 ttccagcctt ttcaggccac gtggatcgcc gagcacggcc tggccgagct tggcgcgatc
    24301 gaccttgccg ccagacgtcg tgccgggaaa ggcggcctcg atcggctcga cagcctctcc
    24361 ctcatacagc tcgtgcacga gggcatcggc atcgcagacc ggcacaccga gctggcggaa
    24421 gcgctcggcc accgccgatt tccccatgcc gatcgagcca gtcagaccga tgacgatcat
    24481 gtcgcactct taccctgtgc tgacccaact caatccggaa tggccttccg cgcacgaagc
    24541 tccttgagaa gggggagcat cggcaagccg aggatggcga agtagtcacc ctcgatccgc
    24601 tcgaatagct gaatgcccgg cccctcgatc tgataagccc ccactgaacc gagcacatcg
    24661 ccaccgacct tggccagata acggccgacg aacgcggggg aaaagttgcg catccacagg
    24721 tcggcagtgt cgacgtgcac ccactccgtc tcgccgtcct cggcgatcac ggcggccgtg
    24781 tgcaattgat gggcacgccc gcgaagggcc agaagcgcat cccgcgcgcg gtccatcgtc
    24841 cctggcttgg tgaacagctc agaaccgcaa gacagcacct gatcggcgcc gatcacgatg
    24901 gatcccggat gccttgcgct cacatcctct gccttcgcgc gcgccaggac ctcggccatg
    24961 tcggctggat cgacctcggc tccgtcggcc gtcaaagtct cccgaatgac cgtttcgtcg
    25021 acgcaggcgg caacggcttt gaacggaatg cccgcagccg tcaacagccg ctgccgcgta
    25081 gggctggcag acgctaaaat gatctgcggg aaggaagcgg actcagccat cgttctcctc
    25141 cgcctggctc gtattctggg cttggcgact ctgatagagc ttcaggatgg tgacggccgt
    25201 ctcctccacc gaccgccggg tcacatcgat caccggccag ccatgacggg cgcagagtcg
    25261 tcgtgagtga gccagctcct gggcgatctg ctcgcgatcg acgtaatcct cgaggttgcg
    25321 gtcgctcatg agccgcacac gattgcggcg aatctcggcg atgcgatcga cgctggccac
    25381 caagcacacc acgaatgccg aatgcggccg ggtgagcggc tcaggcagcg gcagctggga
    25441 gacgatcggc acgttggccg tcttgtagcc gcgctgtgcc agatagatgc tggttggcgt
    25501 cttcgacgtg cgcgatatgc ccaaaatgat gatatcggct tgattgaggt cgtccggaag
    25561 atgaccgtcg tcgtgggcca tggaaaagtt gagcgcatcg atgcgccgga aatagtccgc
    25621 atccaacacg tgctggccgg cgacgatggg cgtctgcggc tccccgagat agctctcaaa
    25681 gactttcatg atgggttcga gcacatacag gcaaggcacc gccagctccc ggcaccgttg
    25741 ctcgagccgc tcggtcagct cgcggttgac gatcgtgtag aggacgatcc ccggcgcgga
    25801 gctgatctcc tgcagcacgc gctccagttg ccgagcggtg cgcaccagcg ggtgcacgtg
    25861 ctcgatcggg cgaaccttgg cgtattgcac cgacgctgcc ttcgcgatcg tggttagcgt
    25921 ctcgccggtt gaatcggaga caagatgcaa gtggaagtag ctcggaaggc ggtggggcat
    25981 ctgtggacaa agtctccggt tgtgcacgcc ggcgggcgct cttcctcgat cagggcggcg
    26041 gggctgctag tcatccacag aacgatccac catcgatctg atccgccaca aaccggcgac
    26101 cgttctccaa tgccgtggat tctgtgcaat gtgcacatcc cttgggcggc gtttcaacac
    26161 ttggttcacc ccactcgagc ccgctgtaaa ggcggaacat gttgactctc aatacggtta
    26221 tcgagcctag tagccgtgcg tcgaggatca caccaaatct tgtcggccgt cgccgggttc
    26281 cgggccagcc tgtgggcagg cgggcagaac gcgacaatta ccggcatccc cggagccaag
    26341 agtcaaaaca gcaagagtct tcaatctcat tgtctgagaa agagataatg cgtgaaatcc
    26401 cgcaggcact taccgagcgc gcaataacct gtccgttcct catgccgttc gctcggctgt
    26461 cccctccaag tcctccgatc tggctgatgc ggcaggcagg ccgctatctg ccggaatacc
    26521 gggcggtgcg gcagcaggcc ggtggcttcc tggatctctg ctacaacccg gagctcgcat
    26581 cagaggtgac gctgcaacct gtccgccgct tccatttcga cgcagccatc ctgttttcag
    26641 acatcctcgt cgtcccgcac gccttgggac agcacgtcac tttcgccgag ggggaaggtc
    26701 cacgtctcgc gcctattcgc tcgcgggagg acatcgaccg gctcgacaag ttgcaggtgg
    26761 gcttcacatt cgctccggtg cacaagacgg ttctgcggac gagggcagct ttgccgcgag
    26821 acaccgccct gatcggcttc tgtggcgcgc cgtggaccgt ggcgacctat atggtaggcg
    26881 ggcgcggatc acccgatcag gccgaggcgc ggctgatggc ctatcgggag ccggagacct
    26941 tcgcgcatct gatcgacatc gtgaccgagg tttcgatcaa gtacctcatc ggccagatcg
    27001 tcgccggcgc cgatgccgtt cagatcttcg acagttgggc gggcagtctc cctgacgatc
    27061 agttcgagcg atgggtcatt gcaccgaccc ggcggatcgt gagcgagctc aaggcgcatc
    27121 atcaggagat ccctgtgatc gggttcccgc gcgccgcggg cgagaagaca gcccgatacg
    27181 tccgtgagac gggcgtcgat ggcgtgagct gcgacacggg catgtccctc gatgccatgg
    27241 cggcgctcgc cgacacggag gacgttgtcg tgcagggcaa tctcgatccg ctgctgctgg
    27301 tgaccggcgg cgagccactc gaagcgcgtg tccgccagat tctggctgcc atgcagggcc
    27361 ggccattcat tttcaacctt ggccacggca tcgttcccca gacgtcaccc gagaacgtgg
    27421 ctcggcttgt cgcgctcgtt cgcagctagc tggggaggtg cttcgtggag ctctggatca
    27481 aggcactcca catcatcagc gtcatcgcct ggatggcggg gatgctctac ctgccgcgcc
    27541 tgttcgtcta ccacgcggcc tcgaaacctg gctcggagca gtcggaaacc ttcaaggtca
    27601 tggagcggcg cctgctcagg ctcatcatca acccggcctc gatcgtcgtc tggatcaccg
    27661 ggccttacct cgcctgggcg tgggggatgt acatggatgg ctggttgatc gcgaagttca
    27721 tccttgtcct tgcgttgacc gctctgcacc atgcgcttgc catctggcgc aagcagttcg
    27781 ccgaggaccg caaccgccac gaccagcgct tctaccgcat cgtcaacgag atcccgacgg
    27841 tgctgttgat cgggatcgtc atcctggtgg tcgtgaagcc tttttaacag gcacctcgtg
    27901 atggcatctt tttctgagcc ggttcatctg ctatataata tccgaacgac ggtcgccggc
    27961 ggctgagagg tttgccggtc cgctgcctgg atttcccaca tctcctggta gaccgcacat
    28021 tcggcgccaa tccgcgccca actcccactt tctgaattcg gcccggtttg aaacctctcg
    28081 cccgccttag cgccgtgccg gtcggagtca cccgatgact gaactgaaac tgcaagacct
    28141 gaaatccaaa tctcccaccg aactgttgag ctttgccgaa gagcatgggg tggagaacgc
    28201 cagcaccatg cgcaagcagg agctgatgtt cgccgtcctg aagcagctcg ccggcaagga
    28261 aatcgaaatc acagggaccg gtgtcgtcga ggtgctgcag gatggcttcg gcttcctgcg
    28321 ctcgcccgac gccaactacc tgcccggtcc cgacgacatc tacgtctctc cctctcagat
    28381 ccgccgcttc gccctgcgta ccggagacac ggtcgaaggc cagatccgca gccccaagga
    28441 gggggagcgc tacttcgcac tcctgaaggt caacaagatc aatttcgaag atcccgacaa
    28501 ggggcgccac aagatccact tcgacaatct gacgccactc tatccgacgc agtggctgaa
    28561 gatggagatc gaggacccga cgatcaaaga tctgtcggcg cgggtcatcg acatcgtggc
    28621 ccccatgggc aagggccagc gcgctctcat cgtcgctcag ccgcggaccg gtaagaccgt
    28681 actcctgcag aacatcgccc actcgatcac gaccaaccac ccggagtgct acctgatcgt
    28741 gctgctgatc gacgagcggc ccgaggaggt gacggacatg cagcgctccg tcaaaggcga
    28801 agtcatctcc tcgaccttcg acgagccgcc gacgcggcac gtgcaagtct ccgagatggt
    28861 gatcgagaag gccaagcggc tcgtggagca caagcgcgac gtggtgatcc tgctcgactc
    28921 gatcacccgc ctgggccgcg cctacaacac agtcgtgccg tcctcgggca aggtgctcac
    28981 cggcggcgtg gacagcaatg cgttgcagcg gccgaagcgt ttctttggtg ccgcgcgcaa
    29041 cgtcgaggag ggcggctcgc tcaccatcat cgccacggcg ctgattgata ccggctcccg
    29101 catggacgag gtcatcttcg aggagttcaa ggggaccggc aactgcgaag tcgtgctcga
    29161 ccgcaaggcg tccgacaagc gcgtctatcc ggcgatcgac atcgcgcggt ccggcacgcg
    29221 taaggaagag ttgctcgtgc cacgcgatca gctcaagaag atgtacgtgc tgcgccggat
    29281 cctgaacccg atgggcacga tcgacgcgat cgaattcctg acggacaagc tccggcagac
    29341 gaagaacaac gccgagttct tccaatccat gaacgcctga cggcgctgac atacgaggac
    29401 cagcatgggg gaccgggtga acgctttcgg gcagccgatc ggctttccgg ttccccagtg
    29461 gtcgccgagg cctcggccgt cccgcatcgt tctcgacggc cggttctgtc gcctggagcc
    29521 gctcgatgcg gaacggcaca cggccgacct ctacgaggcc aaccaggccg atgacggacg
    29581 cggctggacg tatatgagtc atggcccatt cgcgtcgctc gccgattaca gggattgggt
    29641 cgagaaggcg tcggcgtccg aagatccgtt gttctatgcg atcctggagg ctgggtccgg
    29701 tcgcgccgtc ggtgtcgcga cgctgatgcg catcgatccg ccgaacggcg ttatcgaggt
    29761 cggtaacatc aagtattcgc cggctctgca gcggacgccc gccgcgacgg aggcgatgtt
    29821 cctgctcatg cgctatgtct tcgacgagct gggctatcgc cgctacgagt ggaagtgcga
    29881 ttccctcaac cagccgtcac gagccgccgc cttgcgactg ggcttcacct tcgaaggcat
    29941 cttccgtcag gcggtcgtct acaagggtcg caatcgggac acggcctggt tctcgatcat
    30001 cgatgccgaa tggccagccc tgaagcaggc ttacgagcgc tggctctcgc cggcgaactt
    30061 cgatgccgat ggccgacagc agcgccgtct ctcagacctc atcgctgaag cacgttcggc
    30121 accttcgcct tcaggccggt gacgatctgg ccttccgctt gtggtggagc aagaaggcga
    30181 gggcttctgc atctgtaatc ggcgccgagc aggtgtgtcc gatgcacacg taagccgtgg
    30241 cctttccttc caccgggcgc ttgccggcca gcgagtcggc ctccgcgatc acggtctccg
    30301 catcttcgac gaagtgctgg agagcgcccg gcagcgacat gctcgtcacc gtcttttcga
    30361 gggttgcggc gccagcctcg tcaccgccgc tcagcacgat gacgtgctgc ggagataaaa
    30421 ggtcgaacgt ctgggccaac agcgccgagt gcgcgatcag gttctgctgc agctcgccgc
    30481 cgaacgcatt gagtatggct tcggcgcgct ccagatatcc cgtctcgccc ttcagcatga
    30541 agagatggac gagatttgac agcatcacgg cgttggcgtt cggcgtggcg tcatcatgcg
    30601 cggacttcat acgaacgatg acgtcgggcg tatcgtccgc cgtcatgaag tagccgccgg
    30661 agcgcgcatc ccagtaatgc tggtcgagtt gctccacgaa ccgaacggcg gcgtcgaggt
    30721 agcgggcaga gttcgttgct tgggccagcc ggagggcggc ccaggtcatg ttcgcgtagt
    30781 cgctcgccgt tgccggcgct ttcgcctggc cggccctgta ggaatgcacg agccggcccc
    30841 cgctcatcat gcgatcttca atgaacgcaa aggctctctc ggcgagatcc cgccaatggg
    30901 gctcgcggaa aacgaaagcc gcgcccgcga gggcagcgat catgagaccg ttccagtcag
    30961 cgaggacctt gtcatcccag cccggccgga cccgctccgc gcggcgggcg aagagcttgg
    31021 cccgtaaggc ggcgaggcgc gcctcgtccg cctctgagcg cagctccagc gcaccaagcc
    31081 ggttcaggat gttgtgccct tcccagtttc cttcctgtgc tacgccgtag atctcgttga
    31141 agaagcgggc gtcatccggc cccagcacat ccgcgatttc agcggcgctc cagacataga
    31201 acttgccttc ctcgccttcg gagtcggcat cgagcgaggc tgcgaacccc ccgccctcgc
    31261 tgatcatctc gcgctcgagc caactgacag tctccgcgat ccgctgtttg aacagcggat
    31321 tgcccgtttc acggtaaacc tcggtcatga ggtcgatcag cagggcgttg tcgtagagca
    31381 tcttctcgaa gtgcgggacg agccagtgct catccaccga gtatcgcgcg aaaccgcctc
    31441 cgagatgatc gtagatgcca ccttggcaga tgtgggtcag cgtcttctcg acggccgcct
    31501 ccgcctcagg catgtcgtag cggatcgcgc cgcgccacat gagccagaag aagctccact
    31561 gcggaaattt cggagcgccc tggaggccgc cattgacgac gtcgactgcg cgcaccatgc
    31621 ggcctgtcag ctcttcgagc aggggatccg tgatctgcac cttaccggcc gcggcgcctc
    31681 tggccttcaa ggtgtccgtc aggagttccg cgttgtggcg caccttgtcg ggctcctcgc
    31741 ggtagatccg ctcgacctcg cgcaggacat gctggaaggc tggcttgccc cagcgggact
    31801 gcggcgggaa gtaggtgccg ccccagaacg gccgcccgcc tggatcgagg aacatggtca
    31861 gcggccatcc gccctgctcg ccaagctcat gaagagcatt catatagatg gcatcgatgt
    31921 cgggtcgttc ctcgcgatcc accttgatgt tgatgaacag gtcgttcatg agagccgcgg
    31981 tagcctcgtc ctcgaagctc tcgtgagcca tgacgtggca ccagtggcag gcggcatagc
    32041 cgatggaaag caggatcggc ttgttggtcc gcttggcctc ggcgagggct tcagggcccc
    32101 aggcccacca gtgcacaggg ttgtccttgt gctggagcag gtaggggctc gtttcccggc
    32161 tcagcctgtt ttcgctcatg gcatcctcct gctcgttcgg ttttgatcgg cgccggctgg
    32221 cgtacccata cgggtgactc cgacactatg atcgaggttg caaccatatt cgcactttcg
    32281 agtgcgccag gccgggccgg tgtcgccgtc atccgcatat ccggaccggc atcaggcagg
    32341 gtgctggata tgctcgcatc gccacgccca gagccgcggt tggctgcctt gcgccgcctg
    32401 cgcaatccga gctccggcga ggtcatcgat caggctcttg tcctctggtt cccagcgccc
    32461 ggcagcttca ctggggagga cgtagccgag ttgcacgtcc atggcggtcg cgccgtcgtc
    32521 caggccgtgc tctcggccat cggcgatgtg ccggaatgcc ggccggccga agcgggggag
    32581 ttcgcccggc gcgccttcga gaatggcaag ctcgacctca ccatggccga gggcctcgcc
    32641 gacctcatcg acgcggagac ggaagcccaa cgtaaacagg ctctccgtca ggccgagggc
    32701 gcgcttgcag acctctatga tgggtggcgc gattcgctcc tcgaagccct tgcgctcgtc
    32761 gaagcggcga tcgatttttc ggacgaagga gatgtcggca cgacgacttt cgagcgggcg
    32821 gtgccgatcg tgcgcgatct cgagacttcc attgcccacc atctggcgga cgggcacagg
    32881 ggcgagatcc tgcgcgaagg cttccaggtc gtgctggcgg ggccgcccaa cgtcggcaag
    32941 tccagccttt tgaacgccct cgcccgccgg gacgccgcaa tcgtctcgga agaagcgggc
    33001 acgacgcggg atgtgatcga ggtgcggctc gatctcgaag ggctgccggt gatcatcagt
    33061 gatacggccg gcattcgtga ggccacaggt gtcgtcgagc gagaggggat ccgccgaacc
    33121 gtggcgcgga cgcgggatgc agatctggtc ctgtggctca tggatgcgac tgcgccagag
    33181 cctcatttac cgcccgatct tgccgacatg gcagaccgga cactttgggt caccaacaag
    33241 gcggacctgc gcaccggcgg ctggccgaag acgttgcccg acgatatgtt ggtggtatcc
    33301 gctctaactg gacagggaat cgacaagctg acccggcgga tcgcggcgat cgcccgcgag
    33361 cggatcgcac ctggcgagca ccccggcctg actctcgagc ggcacaggct gaacctgacg
    33421 gagtgtcatc aagctctcgt cgctttccgc gagggcagcc cctggcagct cgagctcagg
    33481 gccgaggatc tgcgcattgc cgccaacgcg ctcggccgcc tgaccggccg catcgacccg
    33541 gaacacgtgc tggatcagat cttcggccgc ttctgcatcg gaaaatagag gcaaatccga
    33601 atgtttcacg tgaaacttca cgcggagttg gtgaaatctt cgggaagcgc ttggattact
    33661 gtcgtcggaa gaactgccca atggatccgg cgcaacgtcc agtcatcgcg taagaagtga
    33721 ccacgtagtt ctttccagtc actgcttccc tcggttagtg tgcggccggc ccgaatagca
    33781 accgcacctc gcatccaagg acctatggac acgaagactt atgatgtcat tgtcgtcggc
    33841 ggcggccacg ccggcaccga ggctgccgcg gccgcggctc gcatgggcgc tcgaacggtt
    33901 ctcgtgacgc ataagtttgc gaccatcggc gagatgtcct gcaatccggc catcggcggt
    33961 ttgggcaagg ggcatcttgt tcgcgaaatc gacgcgctgg atggactgat gggccgagtg
    34021 gccgatgagg ccgggatcca gttccgtctt ctcaaccggt cgaagggtcc cgccgtgcaa
    34081 ggcccgcggg cgcaggcgga ccggagcctc taccgcaaag cgatgcagcg ggctatccgt
    34141 gcgaccgaaa accttgaagt catcgaaggt ggcgtcgacg atctccttgt tagagatggg
    34201 atcgtccagg gggtggcgct gggcgatggt cgcgagctcc ggtcgggcgc cgtcgtcctg
    34261 acgaccggaa ccttcctccg gggcctcatc cacatagggg aggtccagat tcctgcaggt
    34321 cgcgtcggtg aggcgcccgc cgtcaagctc gccgatcgcc tggccgccct ggcgtttagg
    34381 ctaggccgtc tgaagaccgg cacgccggcg cggctgcatc gcgattcgat cgattgggat
    34441 cgcctggaag tccaacccgg tgacgatccg cccgtgccgt tctccttcct caccgagcgg
    34501 atcaccactc cgcaggtcgt atgccacatc accacgacga cagaagcaac ccacgccatc
    34561 atccgcgcca atctggatcg gtcgccgatg tacacaggac agatcggcag catcgggccg
    34621 cgctactgcc cgtcgatcga ggacaaggtg gtccgctttg cggatcgcag ggcgcacaac
    34681 gtgttcctgg agcccgaagg tctcgacgac gacaccattt atccaaacgg ggtctcgacg
    34741 tctctgcccg aggaggtgca gatcgcgttt ctgcggacga taccggggct tgaatctgtg
    34801 gtcatcaagc ggccgggcta cgcgatcgaa tacgaccacg tcgatccgcg ggagctgcta
    34861 ccgacgcttg agacgaagcg attgccgggc ttgttcctgg ccgggcagat caacggcacc
    34921 acgggctacg aggaggcagc ggcccagggt atcgccgcag ggatcaatgc agccctgaag
    34981 gccggcggca gcaggcggac gttcgttgtt tcccgcaccg agggctacat gggggtgatg
    35041 atcgacgacc tcataacacg gggcgtcacc gagccatatc gcatgttcac ctcccgcgca
    35101 gagtaccgtc tgaaactccg ggccgataat gccgatcaac gcctgacgcc cgccggggta
    35161 gagatcggct gcgtcggacg gcatcgcaag gcggccttca agaggaagca gcaggcactc
    35221 gcagaggggc gaaggctgct cgagcggctg acgctgacgc cgaacgaggc ggcgcggcat
    35281 gggctggata tcaatcatga tggacggagg cgatcggcct tcgagttgct ggcctatccg
    35341 gagatgacgc tggaacgcct cgctgctatc tggccggaca tcaggacgct cgagccgaag
    35401 atcgcgtctc agctcgaggt cgacgcgcgt tacgcatcat acgtcaagcg gcaagccgag
    35461 gacgtcgaga cgctgaggcg ggacgaagcg gttgctattc ctttggactt cgattttggc
    35521 acattgcctg gtctgtccaa cgaagtgcgc cagaagctga gcctgcaccg gccggcgacc
    35581 ttggcgcaag cggcgcgcat cgacggcatg acgccggcgg cacttctgat ccttctcggc
    35641 cggttgaagc gctccgatcg gcgccgtcaa agcgcatgac tcgccgtagc ggcatcgagc
    35701 ggatagacga agctggggcc tttcaacgcg cattttcggt ttcacgtgaa accgtggatc
    35761 ggctggagcg gtacgaggcc ctgctgagac aatggcagaa ggccgtcaat ctcgtcgctc
    35821 ctagtacgct ggcggatgtc tggcatcggc atttcgccga cagcgctcaa ctggcggcgc
    35881 tcatcccaga ggccgccgaa agccttgttg atctcggctc gggcgggggc tttcccggcc
    35941 tggtgctcgc gatcgttctg atggagcgga ggccctatcg gttcacgctg atcgaaagcg
    36001 acacgcgaaa ggctgcgttc ctgcgcgaag tcgcgcgcga gacccgggtg cctgtggaca
    36061 tcctcgcgtc gaggatcgaa aaccccgaga ctcagcgtaa agtaggccaa gtggatgtgg
    36121 tcacggtgcg tgctcttgct ccgttgagcc gcctacttgg gctggcggcc ccgttctttg
    36181 cgcctcatac agtcggtttg tttctcaagg ggcgcgatat tcacggcgag atcgacgagg
    36241 caagggctgg ctggcggttc gacgtcgtgc tcgtcccgag caagacggat cgggaaggcc
    36301 agatcgccgt ggtgcgcaat gtgtcagcca agacggaggg ttgagccgtg accggcaaga
    36361 ccgtgagatc caagggactg cgggtgatcg cgatcgctaa ccagaagggt ggggtcggca
    36421 agacgacgac ggccatcaat ctgggcacgg cgctggccgc agccggagaa cgcgtgctcg
    36481 tcgtggatct cgatccccag ggcaacgcct cgacgggcct gggcgttgcg aaccaggagc
    36541 gcattcgcac gacctacgaa gtgatgctgg gccaggtccg tctgttggat gcggctctgc
    36601 agacgcaagt gcctggcctt tccatcgtgc cggcgaactc ggacctcgtg ggtatcgaat
    36661 cccagctgat gaacgagccg acacggccgt ttcgtctgcg ggatgccatc ggcgggctcg
    36721 tcggccaaca gcgggccgat gcggaagccg cgattaccta tgtgctgatc gattgcccgc
    36781 cgtcgctcaa tctgctgacg ctgaacgcga tggtcgcggc gcatgccgtg ctggtgccgg
    36841 tgcagtgtga attcttcgcg ctcgaaggca tctcgcagct caaggacaac atcgagcaga
    36901 tcaaggcgac gctgaacccc ggtctggaga tccaaggtgt cgtgctgacg atgcacgatc
    36961 agcgcacggc gctctcgcgc gaagttgcgc agaacgtgcg cgccttcttc ggcgccaagg
    37021 tttacgacac gatgatcccg cggaacacgc gggttgccga ggcgccatcc cacggcaagc
    37081 cgattctgct ttacgacgtg aacagcgcag gcagccaggc ctacatgcgc ctggccgcgg
    37141 agatcatcga gcgggaacgt cacttcaagg ccgcttgagc gtctggccag cgcttgaatt
    37201 acgcgggaga ttagaacgac catggcgact ccactccaga agagccggct tggcaggggt
    37261 cttgcgtccc tcatcggaga tccggctctg gccgagcaga aactgccggc ggaaggcgag
    37321 cagagagtcg tcccgatcga ccagatccgc gcgagcaagc tcaatccgcg gcgggaattc
    37381 aatgaggaag atctggcgga gctggccgag tccattcgcc agaaggggtt ggtgcaaccc
    37441 atcgttgtcc ggccggaccg tctggcgggc ggctacgaga tcgtggcagg cgagcgccgc
    37501 tggagagccg tccagcgcat cggcatgcac tccgttccgt gcatcgtccg ggagttgtcc
    37561 gatcaggagg tcctcgagct cggcattatc gagaacgtgc agcgggctga cctgaacccg
    37621 atcgaagagg ccatcgggta tcgggagctg atcgagcggt tcggctatac gcaggaccag
    37681 ctggccgagg tcatcggcaa gagccggagt catcttgcga atacgattcg cctgctcaag
    37741 ctgccggaga cggttcagag cctggtccag gaaggcaagc tcacggccgg ccatgccagg
    37801 gcactcgttg ggcgagagga tgcagaagcc ctggcgcagc gcatcattga gcgggacatg
    37861 aatgtgcgcg aggtcgaggc gatggttcat ggcgcggtga ctgcagcacc gagccggcgt
    37921 cgggagaaga ccgccgacac gctcgcgttc gaaaaggagc tctcggacgt gctcggcctc
    37981 aaggtcgaga tcaagcgggg ctccggcgag agcgggaccc tcgtcatcaa gtatggcaat
    38041 tacgaccagc ttgattatat ccgcatgcga ctggtcgggt cgcaaggcat gtgacgagcg
    38101 ccgttgggct ggaaagcagg tgtctgcgct ctgtccgagg agagcgtaga gagaagcatt
    38161 cccgtccggc atctttagca tggctccgcg ccgacgcggc gcttgacgcg actggctgca
    38221 ctttgctctt gtccgtgcgc gggcgcacct aggcggccgg cgggagttct tttcatgagc
    38281 catgatgtcc tagcgcgcct ggcggcgacc atccgacagc gtcgctcgga agatgctgag
    38341 cgctcgtaca cgcggcagct cattgacggc ggcccggaaa gatgcgcgcg taagctcggc
    38401 gaagaggcgc tggagaccgt tatcgcaggc atcagccagg atgagcgcgc cctggccaac
    38461 gaagcggccg atttgctcta ccacctgctg gtccttctgg aggcgcgggg cgtcgattgg
    38521 gaagaggtta tcgccgtcct ggaaggccgg atgggtacgt ccggactggt ggagaaagca
    38581 tcacgggcca aacaaggcga agggggcatg tcatgacgct cgctttggtt cggcaggata
    38641 tgcccaacaa gtcggcgatc cagttctcac cctaccgcga gttcagtcgg gaagaatggg
    38701 cacgcctgcg cgccgatacg ccgatgacgc tcgtcgaacg ggacctggag cagctttccg
    38761 gcctgatgca ggagctgtcc atggaggagg tggagcagat ctatctcccc ctctcgcggc
    38821 tactcaacct gtatgtcgcg gccgcgcagg agctgcatgc ggcgactttc cgcttcctgg
    38881 gcgggccgga catgaaagtg ccgtatatca tcggcgtcgc gggctcggtg gctgtcggca
    38941 agagcacgac ggctcgtgtg ctgcgggcac tgcttgcacg ttggccggac catccgcgcg
    39001 tcgatctgat aacaaccgac ggcttcctac tgccgaaccg tgagctggaa aagcgcggcc
    39061 tgatgaaccg gaagggcttt ccggagagct tcgacacggc tcggctgctc aactttctcg
    39121 ccgacgtgaa gtcggggtgc gagcgggtgg aggcgccggt ctactcgcat tttcactacg
    39181 atgttctgcc gggccagaca gtcgtggtgg aacggccgga tatccttatc gtggagggcc
    39241 tgaacgtcct ccagccggcg cgtctaccac gggacggaaa ggcgatcccg ttcgtgtccg
    39301 acttcttcga cttctcgatc tacatcgatg ccgacgcgaa ggtgatcgag gagtggtacg
    39361 tagctcggtt catgaagttg cggacgaccg cgttccgtga ccccgcggcc tatttccacc
    39421 ggtatgcgca cttgtcgcag gaagctgctc aggcgcgggc cctcgagatc tggcggacga
    39481 tcaatctcag gaacctggaa gagaacatcc tgccgacccg acagcgcgca agcctcattc
    39541 ttcgcaaagg ctccaatcat cgaatcgaga gcgtatcgct tcgcaggctc tgaactgggc
    39601 tcgcctgtgg gcgagaggaa ctcgaacggc aagtcgaggc tcctttcctg atggagttag
    39661 cagaaagagg aaacgccgcc cttccgggcg gcgtccttct cctcagctca aacggcgttc
    39721 ccggttatcg ccggctacgc cggacgccac gtctaccgta gaagctgccg cggtacatgg
    39781 cccgtggagc gcgcaccatg cgaggtgccc gatagaagcg ctgagccctt ggcgctctga
    39841 actgcctgcg aggactgaaa taggcgcggg accgataacg ccgtggagcc cggtaggagc
    39901 gccgatagta ccgtcgtgga gcgtagtacc gacgcggcgc gtaataccgc cgatagaatc
    39961 tccgaggcgc gtagtagcgg cgcggcgcgt aatagcgccg atagaacctc cgaggggcgt
    40021 agtagcggcg cggggcgtaa tagcgccgat agaatctccg gggagcgtag tagcgcctgt
    40081 aatatcgggg acgataccag cggtagcctg atgagtaata gaagggcctg tagaagccga
    40141 atcccgccca gaaacgacga tagggacggt accagcggac gggacgatac cagcggtagg
    40201 aatagtagga tggaccccaa taagcaacgc gcggccgcca ggaacgataa tagcggggtg
    40261 cccgatatct ggctctatag aaacgccgtg ggccccagta tgcggcacgg taaaatcttc
    40321 gcggaccgta gaagcgggcc ccgcgataga agcgcgggcc tgcgaagcga cgcactccga
    40381 cgccgcgtac gccacggaag ccccaaccgc gcctgaactg caccttgtcc acgtgggtcg
    40441 atttcgcctc cggggcaaag gaggcgggcg gaggtgccgg tgtggcctga gccgctccaa
    40501 ccagcgtgcc catagccacc gagcacccgg cgagcagcaa tagtcggcga ctgttcatcg
    40561 cgatactcct ctcctgcgac cattgggtcg gtttgctgag catctgaacg tcgcggactg
    40621 ggatgggttc ctgagaggag gtccgttcca ggcaacctct cagctattat tttttgtata
    40681 gcgcgcaagc gcttgcagcg gcgtgttgag ggatggatca gctccctgta tcggctggaa
    40741 gagacaaact cgatggtttg tccttggatt ttcagaggag gccgttcgat cgggccagct
    40801 cggaaagctt gacctggggc ctggcgccga aatggctgat cacttcggcg gcggccatgc
    40861 tgcccaatcg tccggcctgc tccagagagc aaccgcgcgc gagcccgaac agaaacccgg
    40921 cggcatagag atcgccggcg ccagttgcgt cgacgagttc attcaccggt gtggctggga
    40981 tcgcgatcgg ctcctgtccc tggacgcaga tcacggagcc cttctcactg cgcgtcaaga
    41041 cagcaaggtg cgtatcggct tgggcgagcc tggccgcttc ctcgaaggtc ggcacctgat
    41101 agagcgaggt gatctcgtct tcgttggcga tcagaatgtc gatgctgttc ttgacgagat
    41161 ccaagaattc ctgccggtgc cgatcgacac agaacccatc ggacaggctg agggccacct
    41221 ttcgcccggc ctgagcagcg atctgggctg cctgccggaa cgccgccttc gcctcggggc
    41281 ggtcgaacag ataaccctcg agatagacga ttccggcgga ccggatcacc tcgggatcga
    41341 cctcgccccc gttgaggtcg ggactgatgc cgagataggt gttcatcgtc cgctcgccgt
    41401 ccggagtgac caggatgagg cagcgtgccg tggggatgcc ggacgacgat gccgccgtgt
    41461 cgaagtggac gcctgcagcg cggatatcat gggcgaagat ctggccgaac tcgtccgcgg
    41521 ccaccttgcc gatgaaggca gcccgtccgc cgagggacgc aatgccgacc atggtgttcg
    41581 caactgagcc cccggacact tcgacggccg ggcccatggc ccgatagaga ctatcgaccg
    41641 tctcagcatc cacgagccgc atatggccct tgagggcccc atggcggacg aggaaatcgt
    41701 cagtgcaccg gccgatgatg tcgacgatgg cgttcccgat gccgacgacg tcgaagcgcg
    41761 tattggtcat ctcatccccc gcggaatacg tgcgcgcact ataagggccg gtagctgggc
    41821 cgcaacatca ggctcggtaa ctgcgggccg gaggacgatc cgatgcgatg cgcgccaggt
    41881 cgaggatgag gcgctccgtc attggcgatt ccagcgcgtc attcaatctg gccgccttga
    41941 gcgtttcggc gatccgtgaa agggcggtga tcagcttgcc
//
LOCUS       DASVDU010000088        36249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1203052, whole genome shotgun sequence.
ACCESSION   DASVDU010000088 DASVDU010000000
VERSION     DASVDU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 36249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36249
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203052"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF226_05735"
     CDS             complement(<1..822)
                     /locus_tag="VF226_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073525.1"
                     /translation="MDCALDIFFLEAVLNGVLLAGLLALLALGLNLVFGVIDVVWICY
                     AELVMVGMYAIFHFYKVVGLPLPVAMLAGILIVAMAGIILHVVVIRPVLETPPINQLL
                     VTGGVLFFLQAAATMAFGVEFRNIGVQLGSVSFADMYFSWNRIITFVMALAIVFGLWL
                     FLRRTFIGTAIRAVSQDRHIMPLMGVDTRRIYLITSAIGGGLAGLAACLMVLQFDIYP
                     QIGLQFGPLVFMICVLGGLGNMVAGFVAAFLIAQFSTIGGSCFATEWGYAIAFLFF"
     gene            complement(863..1567)
                     /locus_tag="VF226_05740"
     CDS             complement(863..1567)
                     /locus_tag="VF226_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073526.1"
                     /translation="MLVLKDVNAGYGHFQALFDVSMRVEAGEAVAVIGSNGAGKTTLL
                     RVISGLLPATSGSMAMEGVDLRRTPPHKIIEAGIAHVPESRRLFPGLTVEENLRMGAY
                     VRSARAHFSERLDYVYGLFPRLKERRNQLAGTMSGGEQQMCAIGRALMSKPKLILLDE
                     PSMGLAPVIVSQVFDLVRRIRSEGYTVLIVEQNVAQVLKVVDRAYLIEVGRVQKSGSA
                     ADMLASDDIRKAYMGI"
     gene            complement(1576..2304)
                     /locus_tag="VF226_05745"
     CDS             complement(1576..2304)
                     /locus_tag="VF226_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073527.1"
                     /translation="MLTVQGLTKTFGGFTAIKDVSFEVAEGEILGLIGPNGSGKSTTF
                     NCIAGMYVPTAGSIVFDGREIAGLPANRVCHAGIGRTYQIPRPFRNLTILENVALSAW
                     FGQNRSVSRDECWRLAAEALAMVGLPTDAKLTTEGLGAAALKKLELARALATQPRLLL
                     ADESLNGLDHAEMDQAADMLERIRNERGITIVWVEHIMGVLMRVVDRVVVLDHGEVIF
                     DGSPQAAQNDPRVIEVYLGGEAAA"
     gene            complement(2585..3880)
                     /locus_tag="VF226_05750"
     CDS             complement(2585..3880)
                     /locus_tag="VF226_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073528.1"
                     /translation="MFKRTTGAFALGLATLFAAHAATAADKQIKIGVIYDLTGPYAAG
                     GSKAAAVGTQIAIDMINERGGVEGYKIVPVNADAQSKTDVAINEAERLLNDEKVDMLM
                     GIFSSAHCVPMAAKVDAAKKFMWANVCVASDVFKNKNLKYVFRGQVHSDQFGEASCKF
                     IAENAKEKLGKDVKDVRVAIIHEDGPYGAGVAKGNKETCSALGMNIVHEEGYAATSTD
                     LSALVTKLRRARPDVILHTGYNPDITLFLRQAKNAGLKWGALIGHGAGFAQIDKLYET
                     FGKDVDYLYNVDPVAAQLLDPKTLAPGLGEVTQELVKRYKAQTGASEVPTHASQGFNQ
                     SWYFFTDVLPRAIKKYGGIDPEALRQAALDTDIPEGGTIQGYGVKFYPPGHPMAGQNA
                     RSFPVVMQYVGGETKIAWPKSIRTIDPVLPLPAGHTYAP"
     gene            4216..6627
                     /gene="bamA"
                     /locus_tag="VF226_05755"
     CDS             4216..6627
                     /gene="bamA"
                     /locus_tag="VF226_05755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7073529.1"
                     /translation="MMYSVQGMARVTSTFVSILFGGGLGACATGGVRLRGSLMRLLLP
                     IAAGVLAVALATSPAAAANIISAVEVKGNQRISADTIRAQLLISPGEAYDPARADRSV
                     RKLFATGQFADVRIEQRGAKLIVTVVENPILASVKIEGNSAIGTDKLQPLLKLKTGAP
                     YTPAKARADAVQLRDYYLKLGYNQAVVEPRTTAAGEGRIDLVFAIREGELNKVRSITF
                     VGNRAFSDAQLRDVITTTQSGWLDFLKSNLGFDEERLAVDRELLRRHYLKNGYADVEV
                     SPHHAEFDPATKTYAISFKIDEGEQYTFGAVSIESVVPGLDERTLGDALLVKTGEVYN
                     AELIEKSEEKLMAALSAQSQPFAQVKVIPSRDPASRRMSLKFLIEEGPRLFVERIEIV
                     GNTKTKDHVIRRELGFAEGDGVNAFLLRRSAERVRKLGFFKSVEVKQKKGSADNKLIV
                     TVEVVEEETIDLSFGAGYSTSEGVIGDVSVTERNLLGNGQWLQLKLAGSFTRLQADIG
                     FTEPRFLGTRMAAGFDLFYKDFDFSDQSSYKAQKIGGKLRVGIPLTDELNLGLNYKFS
                     RNTIYDVGEDASVAIKEAVSGRDSATFDTSSVGYSLTYDTRDRKKLPRSGIYAGLGQD
                     LAGVGGDVRYVRSVVDVRGYHPLGSSVTLLGRARAGHIVGWGGSEVSLLDMFYGGGDL
                     VRGFAPRGIGPRDSQSRNKDALGGSSYYAVTAEAMFDIPRVTETAGLRASVFADAGSL
                     WGVNSTSASVPGLSGNSASPRVSTGAGLVWDSPIGPLRLDYAVPLVKQEGDKTQALSF
                     GIVPY"
     gene            complement(6676..6762)
                     /locus_tag="VF226_05760"
     tRNA            complement(6676..6762)
                     /locus_tag="VF226_05760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6726..6728),aa:Leu,seq:taa)
     gene            complement(6803..7516)
                     /locus_tag="VF226_05765"
     CDS             complement(6803..7516)
                     /locus_tag="VF226_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HEX7073530.1"
                     /translation="MSWSDQVFRYCERGSDPSFWAEPLNAVSNGAFIIAAVAAGISFV
                     RQPRPEPAPGLAILITLVLVIGVGSFLFHTFATRWSAQADVIPIGIFMLAYLGYALRM
                     YLKLGWVWVAVGLAVFIGALQLAGDVQCRPGLFSSSSAPGPCLNGTAGYVPAFLAMLG
                     IGAVLQVRGHRAASYLLLASATFLISMVFRTVDLELCSLTRVAGSAIGTHFLWHFLNA
                     ATLYLLLMAAVRHGHRPRA"
     gene            complement(7534..8067)
                     /locus_tag="VF226_05770"
     CDS             complement(7534..8067)
                     /locus_tag="VF226_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7073531.1"
                     /translation="MTVEQFIDWAMAQPRGRFELYGGRVVQMSPERTKHTEIKGNAYY
                     ALRAAAQKAGERCHILTDGATVKIDDHTAYEPDALVYCGNRLPGDTVIIPEPVVVVEV
                     TSPSSEARDAGIKLAGYFKLPSLVHYLIVDPDDRLVVHHRRGEDGTIVTRVVEHGEFV
                     LDPPGLTLRLADLFPAD"
     gene            complement(8354..10219)
                     /gene="aspS"
                     /locus_tag="VF226_05775"
     CDS             complement(8354..10219)
                     /gene="aspS"
                     /locus_tag="VF226_05775"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX7073532.1"
                     /translation="MAEDKVTSPSGSGSAGEAAHEIPTHRYRTHTCGALRKSDVGKTA
                     RLSGWVHRVRDHGGLLFIDLRDHYGLTQVVADPDSKAFKAAESVRSEWVIRVDGAVRE
                     RPEGTVNPELPTGEVEVYATEIEVLSEAKELPVPVFGEPDYPEDLRLTYRFLDLRRET
                     LHGIIMKRLGITRSIRRRMHEAGFNEFPTPILTASSPEGARDFLVPSRIHPGKFYALP
                     QAPQQYKQLLMISGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVEMSFVEQEDVFQTM
                     EPIIRGVFEEFGEGKPVTPVFPRIPYEEAIRKYGTDKPDLRNPIEMQDVTEHFRGSGF
                     KVFAGMIEKDPKVRVWAVPAPSGGSRAFCDRMNSWAQSEGQPGLGYIFWREGEEGGAG
                     PIAKNIGPERTGGIRDQLGLKVGDACFFVAGDPVRFYKFAGLARDRVGRELNLIDENR
                     FEFAWIVDFPFYEWNEDEKKVDFSHNPFSMPQGGSAALDAATTVEEQLKIKAYQYDLV
                     CNGYELASGGIRNHQPDTMIKAFGIAGFGADDVEKRFGALYRAFQYGAPPHGGMAAGL
                     ERMVMLLTGVKTVRDVALFPMNQQAADLLMGAPSEATPKQLRELHIRLALPERKG"
     gene            10440..11603
                     /gene="rnd"
                     /locus_tag="VF226_05780"
     CDS             10440..11603
                     /gene="rnd"
                     /locus_tag="VF226_05780"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX7073533.1"
                     /translation="MRVITTNSDLAEACARLARHPFVATDTEFLREQTFWPVLCLIQM
                     AGPEDEYIVDPLAPGLDLKPFYDLMANERVTKVFHAGRQDIEIVYARAGLIPSPIFDT
                     QIAAMVCGFGESVSYVNLVKKLTNKDVDKSSRFTDWSRRPLTQKQLAYALADVTHLRE
                     VYLKLKAELESSGREHWLSEEMATLSDPATYETKPENAWLRLKMRTRNRRSLGVLIEL
                     AAWRERAAQMQDVPRSRILRDEALYDIANQAPTDTAKLGELRTLSEGFSRSARAREIV
                     EAVKRGLERDPRTLPQIPTGQPLSAEATALVDLLRVLLKAAAARNRVAPKLIADTSDL
                     ERIASEDDPDVPALRGWRRKLFGADALKLKRGEVALTVAGGEVVAVPTATAKV"
     gene            complement(11662..12039)
                     /locus_tag="VF226_05785"
     CDS             complement(11662..12039)
                     /locus_tag="VF226_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEX7073534.1"
                     /translation="MTATDPVRARSLLIEPELLDKLPQVVKSELAKLPPERQAEFAEE
                     FKRKLASTPAAYLCSLLFCHYGYLGRWGMTIIMWIASIVTFGIVGLIWWLIDLFRIPR
                     MVANYNKDVAVAVMRDLKIIHGS"
     gene            12247..13758
                     /locus_tag="VF226_05790"
     CDS             12247..13758
                     /locus_tag="VF226_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073535.1"
                     /translation="MATSLQQQHYRAYQPRPLTKAEIGNVEILYGGLHWRAEHVMQAV
                     FENMGYKARPLPVATRNDLLLGRELADIGQCCPTSFTTGNLANFLREEAKRIGAEAVA
                     DKYVYVTAGSCGACRFGQYHQSYELALRNIGLEAFRIFLVAQNRMDQGALEGGGFEVN
                     IPLTLGVVWAILCTDALQSMEYRTRPYEVIPGQTDRVVKESVDYLCEVFRNRPVKGEK
                     WGPLAWHLTTNYFIKALREVYRKFDAIEVDRLQVKPVVKITGEFYLQTVEGDPNYNIH
                     RWLESEGAEVYPAAIVVWLDYWIRFYVQELEDRIGTDRTAKAKIWALKGLQKVFRGTY
                     NRMRKALGNIPHEIPDQYELRQLAAPYFHHRLSGGEGDMLIGKALWAHLHNKAHMICE
                     LSPYSCMPNTMSVGAMAGVVGQHPDLLYAPIEVKGDAEVHALSRCQMILTEAKKRAVR
                     EYVKAMEKTGLDPEAVKKVLERKPEMRKATYHVPHMGAAGTAANTMLHVADIS"
     gene            13964..16126
                     /locus_tag="VF226_05795"
     CDS             13964..16126
                     /locus_tag="VF226_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX7073536.1"
                     /translation="MKTVGMDVGSTTIKAVVVEGGEVLWRDYQRHNTKQAEKALEFLI
                     RMEQEVGLTEGRDRIFFTGSGAGLIAPLVGGKLIQEVVAVAASVEKMHPDVHFVSEIG
                     GEDMKTLFFKESGAGKSKQIYMQSACSGGTGTFIEKTARKLRIPAEQLSTMPYDGMSL
                     HKVSSKCGIFAEADANTLLKGGVPVEEIIASLFEAVVYQNLATLTKGNTPLPQVLLLG
                     GPNLFFKGLQQAWRHHLTNLWQDRKCELPPGADPADLIHVPDDALYYAALGCVEVASG
                     EAEGVGLYQGTERLSWWIDEGQHQEKAKSGAKALIREGDDLASFKAAFEASRGSTAAS
                     ARSSDGPVFIGCDFGSTTAKAVVMDSEKEILASVYVQSKGNPIEDAKALFRDIREKAR
                     GSVAGLALTGYGKDLLKDILGADTGVVETVAHATSALHIFPDADVICDVGGTDVKIMI
                     LRQGTVSDFRLNSQCSSGNGAFLQGVAERYGIALEEYADKAFQARAMPGLAMGCGVFL
                     QSDIVNQQRKGWSAEEIMASLAAVLPLNVWIYAGQLQNLRAVGRKFVLQGGTHRNMAV
                     VKAQIDFIKSKVPDAEILIHPYSGEAGAIGAALCAIDAHVEGRPSRFRGFDLIEQLEY
                     RSTTSEDTICRWCPVNCKRAFIDVKLDGAKGRPWSKVPVEPGWERVISGNSCPKGLME
                     DANEMRAVKAQMEKQRRELPNIGEMVREQAFRRAWAEA"
     gene            16059..17264
                     /locus_tag="VF226_05800"
     CDS             16059..17264
                     /locus_tag="VF226_05800"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX7073537.1"
                     /translation="MNFPILAKWFANRPSGGHGPRPERAERAKLRVGIPKVLNIWTTH
                     QFWLGFFASLGVSRVVFSSDTSEEQGREFGSGRGTVDCCYPVKCISGHYGELIFGQKR
                     KIDILFSPMIYSMPSFLKGHVADSLACPRVMAAPENIKAGFLKEKDVFAEQGIRYVAP
                     LVSLADPMQVPRQLFKAFDGVLTGLTFDEVREAVDQGYRTLAGFDREMRARSRKILEW
                     CVREQRPCVMVLARPYHMDAGIGHEIEADLQAYGYPILWMQYFPTDPDLLDWMFGPEI
                     RDGYIKSVLDISDVWPSSYSANTNEILWGAKAAARMPWITCVIRLTSYECGMDQPTFT
                     PVQQIVERSGTLFFSFQELDSTKPAGSVKIRIETIAHYLERNSPTIIARKLAAMPEGC
                     PLLPSAAPA"
     gene            17435..18496
                     /gene="lpxD"
                     /locus_tag="VF226_05805"
     CDS             17435..18496
                     /gene="lpxD"
                     /locus_tag="VF226_05805"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7073538.1"
                     /translation="MEHPGFFERAGPFPLAEVARAAAAELAPGADGDALISDVRSLAD
                     AGRDHVSFLDNRKYLPQLSATRAGACLVSPAFAARVPEGTAALLTKTPYRGFALALQL
                     FYPDALRPKVARPGGTRLVDPSAQLEDGVVIEPGAIIGPEAMIGRGTVVAAGAVIGYR
                     CAIGRDCFIGPNASVTHALVGNRVILHAGVRIGQDGFGFAMGPQGHLKVPQIGRVIIQ
                     DDVEIGANTAIDRGALNDTIIGEGTKIDNLVQIGHNVVIGRHCVIVGHCGISGSTELG
                     DFVVMGGQSGTVGHIRIGTGAQVAGASHPKEDVPAGARVGGTPARPLKEWARELAVLS
                     RLGKQGYVTPGTDGDEGGN"
     gene            18552..19052
                     /gene="fabZ"
                     /locus_tag="VF226_05810"
     CDS             18552..19052
                     /gene="fabZ"
                     /locus_tag="VF226_05810"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX7073539.1"
                     /translation="MNDATAKEQLGTADIARLLKLLPHRYPFLLVDRIFDMDRDESAV
                     GLKNVTINEPFFQGHFPSYPVMPGVLIIEGLAQTAGALCVHSLGESYRPQLVYFMGID
                     KAKFRRPVVPGDQLFFHVRKIRSRGRAWRFYGEAKVEGQVAAEAEISAMIVDTPETQA
                     IASQNG"
     gene            19045..19848
                     /gene="lpxA"
                     /locus_tag="VF226_05815"
     CDS             19045..19848
                     /gene="lpxA"
                     /locus_tag="VF226_05815"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX7073540.1"
                     /translation="MADAQIHPTAIVESGAKLGAGVKIGPYCMVGPEVTLGDGCELIS
                     HAVVAGRTTIGPRTRIFPFASIGHQPQDLKYRGEPSTLTIGADCIIREGVTMNPGTEG
                     GGLETVVGNGCAFLANSHVGHDCHVGNHVIFSNNVMLAGHCTIGDYAILGGGAAVIQF
                     ARVGAHSFLGGMSGLENDLIPYGMALGNRAYLSGLNIVGLQRRGFSRETIHDLRRAYR
                     LLFAAEGTLKERVDDVAAEFADHALVQEIVAFIRAGGKRSLCTPRDSEG"
     gene            19848..21137
                     /gene="lpxI"
                     /locus_tag="VF226_05820"
     CDS             19848..21137
                     /gene="lpxI"
                     /locus_tag="VF226_05820"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HEX7073541.1"
                     /translation="MVSAGDGPLGILAGNGALPLEVADEVSRNGRPVHIVAIAGEAEP
                     GIERHPHTWVGLGQIGGLLRAFRGAGCRDIVILGGVRRPDLKRLRADLGFVINLPLIL
                     SLMAGGDDSVLRKIVSFFEWRGFRVVGAHDVAPGLLAKDGPLGNRKPDDRVSRHIALG
                     FALVEALGPLDVGQGVVIAGGRPVAIEAAEGTDRMLMRLESLRASGITSGGVLVKAPK
                     PGQELRIDLPAVGPKTVELASAAGLAGIAVAAGQVLVAERDRLAQRLAETGLFLIGSR
                     TKLPDSVPANDEAAAASRWGRLRWITGKVRSRDIRDAERALQAISSARPFGAGAAAVS
                     VREHVLALEAGEGAEAMLGRVAGLRQWGDRLSGHRRGVAALQPVDADPAHLVQLADTA
                     GLAGLVMAGAWTGRAHLELLAAASRRRLFVLACEDSG"
     gene            21134..22327
                     /gene="lpxB"
                     /locus_tag="VF226_05825"
     CDS             21134..22327
                     /gene="lpxB"
                     /locus_tag="VF226_05825"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7073542.1"
                     /translation="MNDGEDPLRVFLVAGEQSGDALGARLMGALQVKLGDRVTFSGVG
                     GPLMGKEGLETLFPLADVAVMGPLSILPRLPRIIRRVHRTVDAAIAMRPDVVVIIDSP
                     EFTHAIARRIRRRCPSLPIIDYVSPSVWAWRPGRAKRMRRYVDHVLALLPFEPEAHLR
                     LGGPPCTYVGHPLVERRAWLEALDPAPLAERLTLDPERPVLLVLPGSRSSEVGRLMKP
                     FGEAIGLLVGQGARPQTIIPAVPHVRSQIEVALDSWPVRPHLIAGEEDKFRAFKLATA
                     ALAASGTVTLELALTGTPAVVAYKVDAIAARLRFLVKVPSVVLANLVLGENVYPEFIQ
                     EDCSPEKLAAALAPLLGDTPERWRQRDLLARVPERLAVSAASPSEAAAEIVIRRGRAA
                     RPRRG"
     gene            complement(22352..23677)
                     /gene="gltA"
                     /locus_tag="VF226_05830"
     CDS             complement(22352..23677)
                     /gene="gltA"
                     /locus_tag="VF226_05830"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7073543.1"
                     /translation="MNVHDSASGKADATRTVTLNQNGKQVSLPVRAGSIGPDVIDVTR
                     LYRETGCFTYDPGFTSTANCSSKITYIDGEEGVLLYRGYPIEQLAEQSSFIEVCHLLL
                     YGELPTREQLDWFRDTITRHTMVHEQMTRFFTGFRRDAHPMAIMVGTVGALSAFYHDS
                     TDISDPVQRMIASHRMIAKMPTIAAMAYKYSIGQPFIYPRNDLDYAANFLQMCFAVPC
                     EPYFADPVLARALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAH
                     GGANEAALNMLEEIGTVDRIPEYIAKAKDKSSGFRLMGFGHRVYKHYDPRARVMQKTC
                     HEVLDALGMKNEPLLKVAMELERIALEDDYFIEKKLYPNIDFYSGITLRALGFPTSMF
                     TVLFAVARTVGWIAQWKEMIEDPEQKIGRPRQLYIGATRRDYVPIENRG"
     gene            complement(23901..25340)
                     /gene="gltX"
                     /locus_tag="VF226_05835"
     CDS             complement(23901..25340)
                     /gene="gltX"
                     /locus_tag="VF226_05835"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7073544.1"
                     /translation="MSTGTSQGSKVVTRFAPSPTGFLHIGGARTALFNWLYAKHTGGT
                     FLLRIEDTDRERSTKEAIAAILDGLKWLELDWDGEPLFQSTRADRHREVVEELLRRGN
                     AYRCYCTPEELEAMRKEAEAAGRPPVYNGYWRDRDPSEAPPGVKPAIRLKTPRTGETV
                     IVDQVQGRVVFQNENLDDLIILRSDGTPTYNLAVVVDDHDMGITHVIRGVDHLTNAAR
                     QTQIYNAMDWDVPVFAHVPLIHGPDGAKLSKRHGALGVEAYRAMGYLPAALRNYLVRL
                     GWSHGNDEIFSTQQMIDWFDLDGIGRSPARFDFAKLEDLNGHYIRNTPDDELLARIRQ
                     ALPEFENGPQLEARFAAAGWEKLAAALPSLKPRAKTLKDLVDGAGYLIAERPLVLDEK
                     AAKLLTDEARVLLGRLLPQLESAEWTAGALESEVRSFAEREGVKLGGIAQPLRAALTG
                     RTVSPPVFDVMAVLGREEALARIRDQASA"
     gene            25431..27752
                     /locus_tag="VF226_05840"
     CDS             25431..27752
                     /locus_tag="VF226_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEX7073545.1"
                     /translation="MSLGEGTVDAVRRARERSRALAPGRWLLDRLLAEQDRWFLWTPV
                     LFGLGILIYFRLPAEPQIVAALAPVMCVFALAPMARRATAGLLAINAAIAVSLGFAAA
                     KMRAEWVAAPVLERQLYNSGVEGFVEVVEPRPTRGQRITVRVSRLGKLPPDKRPHRVR
                     IRLMSEMPGLEPGQAVRLKATLAPPSMPALPGDYDFARAAWFSGLGGVGYSMARPEII
                     ADAGEAPWSLKLYAMTQRLRNEIGRRILAALPGEEGAIATALITGERGSISPETTAAF
                     RDSGLVHILSISGLHMVMMAGSVFFSVRLFLAAIPAIALRYPIKKWAAAAAILGALGY
                     LLISGNSFPTIRAWITISIVFGAIMLDRPALALRNVALAALAVLILVPESLFDAGFQM
                     SFAAVVALVAAYELIRDRLERRRSEPGPMFGPVLGFFMFFGGIILTTVIASLAVAPFS
                     AYHFHTSQQYAVLANLIAVPICNIVVMPGALATLLFMPIGLEAVPLWVMEQGIRAMLW
                     CARAVSQLPGAVGHIPAFSDMAFGLMLLGGLWLCLWGTRWRFLGLGVFAAGLALAPMM
                     PRPDILIGNDAELVAIRGVDGKLSALPARGSSYELQRWLENDGDSRTARQAASGEGFR
                     CDSVGCLAILKGKRVAVVHHPAALADDCRMADVLIVPFPKPRGCNPPGLAIDFYAVRD
                     RGTHAVYIADGHLSLETVADARGDRPWSRRPERPPRKTRSEAGNTISRLKGFAAPRDL
                     AEASRRMRAELEDDDDPVVRRLEEEGAELDDTP"
     gene            complement(28004..28711)
                     /gene="lexA"
                     /locus_tag="VF226_05845"
     CDS             complement(28004..28711)
                     /gene="lexA"
                     /locus_tag="VF226_05845"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX7073546.1"
                     /translation="MLTQKQKELLLFIHGRLQETGVPPSFDEMKDALALKSKSGIHRL
                     ITALEERGFIRRLPHRARALEVIKLPENSEPTQPRRSGFTPSVIEGSLGKQSQLSVPP
                     SSMVDSRTISVPVMGRIAAGVPISAIQSHTHDIACPPDLLTNGEHFALEVRGDSMIDA
                     GIQDGDIVIIRRCETAENGDIVVALVEKEEATLKRLRKRGSAIALEAANPKYETRIFG
                     PDQIDIQGRLVGLIRRY"
     gene            complement(28832..30034)
                     /gene="glp"
                     /locus_tag="VF226_05850"
     CDS             complement(28832..30034)
                     /gene="glp"
                     /locus_tag="VF226_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7073547.1"
                     /translation="MALSVSEALEKVLADVAPTSPEYVPIEQAAGRILAEPLIARLTQ
                     PPFDASAMDGYAVRSADVAQLPATLKVIGQAAAGHPFDGEIEPGQAVRIFTGAPLPKG
                     ADAVVIQENTSATDGTVEVREGRPDRAHIRPRGFDFRESEALLFPGRRLGPREVTLAA
                     AAGYGEVSVRRRPRIAVLATGDELVRPGERPGPGQIISSNPLGVAALLTQAGAEAKVL
                     GIARDTRDSLVEHVEAARDADVLVTIGGASVGDHDLVGPVLQSLGMELAFWKINMRPG
                     KPLLHGRLGRQRVLGLPGNPVSALICTRVFLVPLVRALLGLPQEDAATVTVPLAEPVE
                     ANGARQHYMRAILERLPDGRQQVRLARSQDSSLLATLATSNVLLVRPPNAPPLPAGTE
                     VTVLLLDF"
     gene            complement(30040..30537)
                     /gene="moaC"
                     /locus_tag="VF226_05855"
     CDS             complement(30040..30537)
                     /gene="moaC"
                     /locus_tag="VF226_05855"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX7073548.1"
                     /translation="MNCGPLTHLNEKGEARMVDVSDKSVTQRTARAEGFVSMQPETLA
                     LVETGQAKKGDVLATARIAGIMAAKRTHELIPLCHPLPITKATVDFELSHQPTGIRVT
                     AEVKVTGQTGVEMEALTAVSVACLTIYDMLKAADKGMTFSGIRLLEKTGGRSGHFRAD
                     DAQGS"
     gene            complement(30534..31349)
                     /gene="trpC"
                     /locus_tag="VF226_05860"
     CDS             complement(30534..31349)
                     /gene="trpC"
                     /locus_tag="VF226_05860"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX7073549.1"
                     /translation="MTGILDRITRYKLEEIRAAKASHPLAEVEAAARAAAPVRPFAGA
                     IEKTLAGGRFALVAEIKKASPSKGLIRADFEPSELARAYEAGGATCLSVLTDGPSFQG
                     APAYLTTARASTNLPALRKDFMLDPYQVAEARAWGADCILIILAQVDDPTARDLAAAA
                     REWGMDAIAEVHDEAELERALQLDCRLIGINNRNLKTFETTLTTTERLAPRVPADRIV
                     IGESGIFTHADLTRLAAVGVRTFLVGESLMRQADVTSATRKLLGLPEEQERTS"
     gene            complement(31360..32385)
                     /gene="trpD"
                     /locus_tag="VF226_05865"
     CDS             complement(31360..32385)
                     /gene="trpD"
                     /locus_tag="VF226_05865"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX7073550.1"
                     /translation="MPTNELRQLIHKVATGATLSAAEIRSALETMTAGLASPAQMGAF
                     LMALRVRGETVDEITGAAELMRSKMTPVEAPPDTVDIVGTGGDGLGTYNVSTCASLVA
                     AGAGVRVGKHGNRAVSSVSGASDVLAALGVKLDVLPPVITDCIMKAGVGFMWAPMHHA
                     AMKHWAPVRAELGIRNIFNLLGPISNPAGVRRQVVGVFDAQWVEPIANVLKNLGSEHV
                     WVVHGHDGLDELTTTGSTKVAELKDGKIRLFEVTPADAGLPPAKLADLKGGNAEANAA
                     AIRDVLAGKPGPFRDVVLLNAAAALIVGGKTQSLTEGVELAARSIDSGAAQHALDRLI
                     AITNGRD"
     gene            complement(32420..33037)
                     /locus_tag="VF226_05870"
     CDS             complement(32420..33037)
                     /locus_tag="VF226_05870"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX7073551.1"
                     /translation="MLVLIDNYDSFAYNLVHFLGELGAACDVHRNDKITVDEVLRSKP
                     KGIVLSPGPCTPNEAGICLDLIAAAAEASVPVLGVCLGHQAVGQAFGGKVVRAPLPMH
                     GKLSELRHTGKGVFEGLPKRFKVTRYHSLMLERASLPSCLEITAETSDGIIMGLQHTS
                     LPVHGVQFHPESIASECGHHLLANFLKIAGLDPRWRNTRPSLKAA"
     gene            complement(33070..33588)
                     /locus_tag="VF226_05875"
     CDS             complement(33070..33588)
                     /locus_tag="VF226_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7073552.1"
                     /translation="MVTLSLLRHGKSSWEDSDLSDHDRPLAAIGRRAAADVGAYIASH
                     DLIPDLILCSSALRARETLDCILPKLGSHKPKVEIEPALYLAPATRMLHVVRQTPASV
                     RHLLLLAHNPGLHGLALDLTGRGLRRDIASMAVKFPTCALAVLTFETDNWHDVHPAAG
                     HLDRFVAPRSLA"
     gene            33793..35214
                     /locus_tag="VF226_05880"
     CDS             33793..35214
                     /locus_tag="VF226_05880"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7073553.1"
                     /translation="MALQLARWSVIVLSVVTAALGAVLGILRAVEPIPDGRIVLATGG
                     SAGIYHELAESYRRELARNGVKLELRQTSEGFATLRGLLDRGSGLDAGFVKGGLVGSL
                     QGRLASERARDWHERELGKLRSVGRMFYEPIWVFTRGDLPIESLRELEGRRILVGTRG
                     SGALRVVRQLLKANGVDEQNSTFIREELPEDAGPLLSGQADAALFILPADSEKVQKLL
                     RVPKIRLMNFEPEAKAYTNRFPALSKVVLRRGGVEFDPLIPSADITLLSTSAALVIRA
                     DLHPALVSLLTHAVIQNPRLGFDKAGDPVLFFRPGEFPSPDDPEFEVAAEARQVYKSG
                     GLPLLLGVLAPLNSRLGLPFFFTAFGASHGLETLLLIIPILTILVPIMKALPVIYRWT
                     MRRRLLYWYRQLKALERNLEDARSQADFAVTQAELERIDARVRRIRVPLGFSDQFYDL
                     RGHIDLVRQRIGQRAPALRMAAE"
     gene            complement(35255..35611)
                     /locus_tag="VF226_05885"
     CDS             complement(35255..35611)
                     /locus_tag="VF226_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073554.1"
                     /translation="MTRRWLAVVPLALLFAPLPASAQVTGLDAIHAQARVGNKVCMTE
                     HEHYGEGTMPTRRAAELLAIRSWSDFTAFEYGRPWGSYRLAAGKRMECSNSGKSYVCK
                     TWARPCRPARLGPPRR"
     gene            35762..>36249
                     /locus_tag="VF226_05890"
     CDS             35762..>36249
                     /locus_tag="VF226_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073555.1"
                     /translation="MPCTFSRLTCWLAVFAALLLSAPAPATAQSCTKDDISRTIDSTA
                     AALRKLNAAHQPELQAKLRQLGELRGWSESEQPTKAAAYIQDDETRALDEQAGRLLLE
                     LEVLGDESAIDKETCLARLEKLKKISAQLIEITTAKAAHVSAKVDAALNAGTAPKRAA
                     EQP"
BASE COUNT         6396 a        11416 c        11983 g         6454 t
ORIGIN      
        1 gaagaacagg aaggcgatgg cgtagcccca ttcggtcgcg aagcaggagc cgccgattgt
       61 gctgaactgg gcaatgagga aggcggcgac gaagccggcg accatgttgc cgagcccgcc
      121 cagcacgcag atcatgaaaa cgagcggccc gaactgcagg ccgatctgcg gatagatgtc
      181 gaattggaga accatgaggc aggctgcaag cccggcaagc ccgccgccga tcgcggaggt
      241 gatgaggtag atccggcggg tgtccacccc catcagcggc atgatatgcc ggtcctgact
      301 gacggcgcga atggcggtgc cgatgaacgt gcgccggagg aagagccaga gcccgaacac
      361 gatggccagc gccatcacga aggtgatgat ccggttccag gagaaataca tgtctgcgaa
      421 ggaaacggac ccgagctgga cgccgatatt ccggaactcg acgccgaagg ccatcgtggc
      481 tgcggcctgc aggaagaaga gcacaccgcc ggtgacaagc agctggttga tgggcggtgt
      541 ttccagcacc ggccggatga ccaccacatg caggatgatg ccggccatgg cgacgatgag
      601 gatgcccgcc agcatggcca ccggcagcgg cagtccgacg accttataga agtggaagat
      661 ggcgtacatg ccgaccatca ccagctcggc gtagcagatc cagaccacgt cgatgacgcc
      721 gaacaccagg ttgagaccga gggcgagcag cgcgaggagg cccgcgagaa gaaccccgtt
      781 gagcacggct tcgagaaaga aaatatcgag cgcgcaatcc atgcaggtct cctgatttcc
      841 ccttcgcaaa agagcagccg cgctagatgc ccatgtacgc cttgcggata tcgtcgcttg
      901 ccagcatgtc ggcggccgag ccgctcttct gcacccgccc gacctcgatg aggtacgcgc
      961 ggtcgacaac cttcagcacc tgcgccacgt tctgctccac gatcagcacg gtgtagccct
     1021 cggagcgaat gcgcctgacg aggtcgaaga cctgcgaaac gatcaccggc gcgaggccca
     1081 tcgacggctc gtcgagcaga atgagcttcg gcttgctcat cagcgcacgg ccgatcgcgc
     1141 acatctgctg ctcgccgccg gacatggttc cggcgagctg gttgcggcgc tccttgagcc
     1201 gcgggaacag cccgtagacg tagtccagcc gctcggagaa gtgcgcccgg gcggatctca
     1261 cgtacgcgcc catacgcagg ttctcctcca cggtaaggcc ggggaacagc cgccggctct
     1321 ccggtacgtg ggcaatgccc gcctctatga ttttgtgcgg tggcgtgcgg cggaggtcta
     1381 cgccctccat ggccatcgag ccggaggtcg ccggcagcag gcctgagatg acgcggagga
     1441 gggtagtctt gccggcgccg ttggagccga tcaccgcgac ggcctcgccc gcctcgacgc
     1501 gcatcgaaac gtcgaacagc gcctggaaat gtccgtaacc ggcgttgacg tccttcagca
     1561 cgagcattgg cgcactcatg ccgcggcctc gccgccgaga tagacttcga tcacgcgcgg
     1621 gtcgttctgc gccgcctgcg ggctgccgtc gaagatgacc tccccgtggt ccagaacgac
     1681 cacgcggtcc accacccgca tcagcacgcc catgatgtgc tcgacccaga cgatcgtgat
     1741 gccccgctcg ttgcggatgc gctccagcat gtctgccgcc tgatccattt cggcgtgatc
     1801 gaggccgttg aggctttcat ccgcgagcag caggcgcggc tgggtggcga gcgctcgcgc
     1861 caactcgagc ttcttcagag cagccgcacc gaggccttcg gtcgtcagct tcgcatccgt
     1921 cggtaatccc accatggcga gcgcctcggc ggcgagccgc cagcattcgt cgcgagacac
     1981 gctgcggttc tgaccgaacc aggcggaaag ggccacgttc tcgagaatcg tcaggttccg
     2041 aaaagggcgc gggatctggt acgtccgccc gataccggcg tggcagacgc gattggcggg
     2101 caagccggca atctcccggc cgtcgaaaac gatgctgccc gcggtcggca cgtacatgcc
     2161 cgcgatgcag ttgaaggtcg tgctcttgcc cgacccgttc gggccgatca ggcccaggat
     2221 ctcgccttct gccacctcga agctgacgtc cttgatggcc gtgaatccac cgaacgtctt
     2281 cgtcaggccc tggacagtca gcaacgccat agagattgcc agctcgcatt ccgtgcttgg
     2341 acggaggtcc cagaattcac gaggccacct ttcggcgacg caatcattac ctctccccat
     2401 gggaagaggt cggctcgaag ggccgggcga ggggttgcac catcagcaac cggtggaacc
     2461 cctttagccc aaccctctcc actagggaga ggggattggt gcactccggt agcctccaat
     2521 ctggaagacc ttgcggtata ccgccggctg caggtgccgg cggcccgttg ggtgacgctc
     2581 gggatcaagg cgcgtaggtg tgcccggctg gcagcggcag gaccggatcg atggtcctga
     2641 tcgacttcgg ccaggcgatc ttcgtctcgc ccccgacgta ctgcatcacg acggggaacg
     2701 aacgggcgtt ctgaccggcc atcggatgcc ccggcgggta gaatttgacg ccatagccct
     2761 ggatcgtgcc gccctcgggg atgtcggtgt cgagagcagc ctggcgcagc gcctcgggat
     2821 cgatgccgcc atacttcttg atcgcgcgcg ggagcacgtc ggtgaagaag taccaggatt
     2881 gattgaagcc ctgggaggca tgcgtcggca cctcgctggc tcccgtctgg gctttatagc
     2941 gcttcaccag ctcctgggtg acctcgccga gtcctggcgc cagcgtcttc gggtcgagca
     3001 gctgggcggc gaccggatcg acgttgtaaa gataatcgac gtccttgccg aaggtctcat
     3061 acagcttgtc gatctgcgcg aaccccgcgc cgtggccgat gagcgccccc catttgaggc
     3121 cagcattctt ggcctgcctg agaaagagcg tgatgtccgg attgtagccc gtgtgcagga
     3181 tcacgtcggg ccttgcgcga cgcagcttcg tgacgagcgc ggataaatcg gtcgaagtcg
     3241 ccgcatagcc ttcctcgtgc acgatgttca tgccgagcgc cgagcaggtc tccttgttgc
     3301 ccttcgccac gccggcccca tagggcccat cctcatggat gatggcgacg cgcacgtcct
     3361 tcacatcttt gcccagcttc tccttggcat tctccgcgat gaacttgcag gaggcctcgc
     3421 cgaattgatc cgaatggacc tgtccgcgga acacgtattt caggttcttg ttcttgaaga
     3481 cgtcggaggc aacgcagacg ttggcccaca tgaatttctt cgcagcatcg accttcgcgg
     3541 ccatcggcac gcaatgggcg ctggagaaga tgcccatcag catgtcgacc ttttcgtcat
     3601 tgagcaggcg ctcggcctcg ttgatcgcca catccgtctt ggattgggca tccgcattga
     3661 ccggcacgat cttgtagcct tcgaccccgc cgcgctcgtt gatcatgtcg atcgcgatct
     3721 gcgtgccgac cgcggcagcc ttcgacccgc cggcggcgta cggaccggtg aggtcgtaga
     3781 tcaccccgat cttgatttgc ttgtcggcag cggttgcggc gtgagcggcg aatagggtgg
     3841 ccagcccgag cgcgaatgcg cccgtcgtgc gcttgaacat gtttcctcct cgacgtccct
     3901 tggagcggat gtgtttttgt gagcgggagc ttggcgcaat tttcatggcg gatcaacgga
     3961 gacgtgagcc gctctcgcgt gaggaggcag cgactgatct atcgggagcg gcctttagtc
     4021 gttcggccgc cgcatgcacg ccatctgcgc ttgcacaaat gagcgacgcc acaaccgtgt
     4081 gttgatagag gcccatacaa tgccccggcg atatctcgat ttggccataa aactcggacc
     4141 atctgccaga gccgctggcc gcggggaggt caggccggcg gaatagagcc atcggacgaa
     4201 aaccgtcctg caccaatgat gtacagcgta cagggcatgg cccgggtgac ctcgacattc
     4261 gtctcgatct tgttcggcgg cggattgggc gcgtgcgcaa cgggcggggt ccgactgcgg
     4321 ggcagtctga tgcgcctcct tctccccatt gcggcgggcg tcctcgcggt ggccctggcc
     4381 acgagccccg ctgcggccgc taatatcatc agcgccgtgg aggtgaaggg caaccagcga
     4441 atctccgcgg ataccatccg cgcccagctt ctcatttctc ccggcgaggc ttacgatccc
     4501 gcccgggccg accgatcggt gcggaagctt ttcgcaaccg gccaattcgc cgatgttcgg
     4561 atcgagcagc gtggggccaa gctgatcgtg acggtggtcg agaatccgat tctggcgagc
     4621 gtcaagatcg aaggaaacag cgcgatcggc acggacaagc tccagcccct tctcaaattg
     4681 aagacaggag cgccttacac gcctgcgaag gcgcgcgctg acgccgtcca attgcgcgac
     4741 tactatctga agttgggcta caaccaggcc gtcgtggagc cacgaaccac cgcggcgggc
     4801 gaggggcgca tcgacctcgt gttcgcgatc cgggaaggcg agctcaacaa ggtgcgcagc
     4861 atcacttttg tcggcaatcg cgcgttcagt gatgcccagc tccgcgacgt catcaccacg
     4921 acgcaatcgg gctggctcga tttcctgaaa agcaacctcg gcttcgacga ggaacggctt
     4981 gccgtcgatc gcgagctgct gcggcgccat tatctgaaaa acggctatgc cgacgtggag
     5041 gtctcacctc accatgccga attcgatccc gcgaccaaaa cctacgcgat ctcgttcaag
     5101 attgacgagg gtgagcaata cacattcggc gccgtctcaa tcgaatcggt tgtgccgggg
     5161 ctcgatgaaa ggacgcttgg cgacgccttg ctggtgaaaa ccggtgaggt gtacaatgcc
     5221 gagctcatcg aaaagtccga agagaaattg atggcggcgc tgagcgcgca gtcccagccg
     5281 ttcgcccagg tgaaggttat cccctcacgc gatcctgctt cccgcaggat gagcctcaaa
     5341 ttcttgatcg aggaagggcc gcgcctgttc gtcgagcgca tcgagatcgt cggcaatacg
     5401 aagacgaagg atcacgttat ccggcgcgag ctcggctttg ccgagggtga cggcgtgaac
     5461 gcgttcctgc tccggcgatc ggctgagcgc gtcaggaaac tgggtttctt caaaagtgtg
     5521 gaggtcaaac agaagaaggg atcggcagat aacaagctga tcgtgaccgt cgaggtggtc
     5581 gaggaggaga cgatcgatct gtcgtttggc gccggctatt cgacctcgga gggcgtgatc
     5641 ggagacgttt cggtgaccga gcgcaatctt ttgggaaatg gccagtggct gcagctcaag
     5701 ctcgccggca gcttcacgcg cctgcaggcc gacatcggat ttaccgagcc gcgctttctc
     5761 gggacccgta tggcagcggg cttcgatctc ttctacaagg atttcgattt ttccgatcag
     5821 tcctcctaca aggcgcaaaa gatcggcggg aagctgcgcg tcggaatccc gctcaccgat
     5881 gaattaaatc tcggcctgaa ttacaagttc agtcgcaata cgatctacga cgtcggcgag
     5941 gacgcttccg tggcgatcaa ggaagcggtt tccggtcgcg attcggccac cttcgacacc
     6001 tcgtccgtcg gctactcgct cacctatgat acgcgcgaca ggaaaaagct gccgaggtca
     6061 ggcatctacg cggggcttgg gcaggatctc gccggcgtgg gcggcgacgt gcgctatgtt
     6121 cggtcggtcg tcgacgtgcg tggctatcat ccgcttggca gcagcgtcac gctcctgggg
     6181 cgcgcccgtg ccggccacat cgtcggctgg ggcggcagcg aggtgagtct cctcgacatg
     6241 ttctacgggg gcggcgacct ggtgcgcggt ttcgcgcccc gcgggatcgg cccgcgcgac
     6301 tcgcaaagcc ggaacaagga cgcgctcggc ggcagctcgt actatgccgt gaccgcagag
     6361 gccatgttcg acatccccag ggtgaccgaa acagcggggc tgcgcgcctc ggtatttgcg
     6421 gatgcaggct cgctgtgggg tgtcaactcg acgtccgcca gtgttcccgg cctgtccggc
     6481 aattccgcct caccgcgcgt atccaccggg gcagggctcg tctgggattc tccgatcggc
     6541 ccattgcgcc tcgactacgc cgttcccctc gtgaagcagg agggcgacaa aacccaggcg
     6601 ctgagcttcg gaattgtgcc gtactgaagc gagcgagacc gagcgcccga tataccgagc
     6661 gcccgatata aagcctggtg cggacggagg gactcgaacc ctcacggcat ttctgcctca
     6721 ggattttaag tcctgtgtgt ctaccgattc caccacgtcc gcgagtcggg ctctttatgg
     6781 cgccccaggg ctgcggtggc aatcacgcgc gcggccgatg gccatggcgc acggctgcca
     6841 tcagcagcag gtagagcgtc gctgcgttga ggaagtgcca gaggaaatgg gtgccgatgg
     6901 cgctgcccgc aacccgcgtc aggctgcaca gctccaggtc gactgtccgg aagaccatcg
     6961 agatcaggaa ggtggcgctg gcgagcagga ggtagctcgc ggcccgatgc cctctgacct
     7021 gcagtacggc gccgatgccc agcatggcaa ggaacgcagg cacgtaaccg gccgtaccgt
     7081 tgaggcaagg cccgggtgcg gatgaggagc tgaagagccc cggccggcac tggacgtcac
     7141 cggcaagctg cagcgcccca atgaagacgg cgaggccgac ggcgacccag acccagccga
     7201 gcttcaggta cattcggagc gcgtacccga ggtaggcgag catgaagatg ccgataggga
     7261 ttacgtccgc ctgcgccgac cagcgtgtcg caaacgtgtg gaacaggaag ctgccaactc
     7321 cgatcacgag caccagcgtg atgagtatgg caaggccggg cgccggctcg ggtcgcggct
     7381 ggcgcacgaa cgagattccg gcagcgaccg ccgcgatgat gaaggctccg ttcgaaacgg
     7441 cattcagcgg ctcggcccag aacgacgggt cggagccgcg ctcgcagtat cggaaaacct
     7501 gatcgctcca ggacaccggc tgcgcccgcc agctcagtcc gccgggaaca ggtcggcaag
     7561 gcgcagcgtc agaccgggtg ggtcgagcac gaactcaccg tgctcgacga cgcgcgtgac
     7621 tatggtgccg tcttccccgc ggcggtggtg caccacgagc cggtcgtcgg gatcgacaat
     7681 gaggtagtga acgaggctcg gcaatttgaa gtagccggcc agtttgattc cagcatctcg
     7741 cgcttcagag gacggagacg tgacctcgac cacgacgacc ggctccggaa tgatgaccgt
     7801 gtcgccgggc agcctatttc cacagtagac gagggcgtca ggttcgtaag cggtatggtc
     7861 gtcgatcttg acggtcgcgc cgtcggtcag aatatggcat cgctcacctg ccttttgggc
     7921 cgcagcccgc aaagcataat aagcattgcc cttgatctct gtatgcttcg tccgctccgg
     7981 cgacatctgg accacccggc ccccatacag ctcaaacctc ccccgcggct gagccatggc
     8041 ccagtcgatg aattgctcaa cggtcatgcg tggtggtgcg tgcacgttca tgggcacaag
     8101 ctaacacaga acacgtgcca gcataacgtg cgggacgttg gtatggtcgc caattcgcga
     8161 gaccgccgaa ttgatgtctc acgacctcaa cgaaatcggt cataccgggg cttgcccccc
     8221 ggtatccact taaccgcttg cgtcagcgat ccctgaaagt tggatcccgg cgacaagcgc
     8281 cgggatgacc tggcgtgagg ccgagtcgtc atgcatcatt tcacgacctc accatttcac
     8341 gacttcaccc tcttcaccct ttccgctccg gcaaggccag ccggatatgc agctcccgta
     8401 gctgcttcgg cgtcgcctcg gagggggcgc ccatgagcag gtcggcggcc tgctggttca
     8461 tggggaagag ggctacgtcg cgcacggtct tgacgccggt cagcagcatg accatgcgct
     8521 ccaggccggc cgccatgccg ccatgcggcg gggcgccgta ctggaacgcg cggtagaggg
     8581 cgccaaagcg cttctcgacg tcgtctgctc cgaaacccgc gatgccgaag gctttgatca
     8641 tcgtgtccgg ctggtgattg cggatgccgc ccgaggccag ctcgtagccg ttgcagacga
     8701 ggtcgtactg gtaagccttg atcttgagct gttcttcgac ggtcgtcgcc gcatcgagag
     8761 ccgcgctgcc gccctggggc atggagaacg ggttatgcga gaagtcgacc ttcttctcgt
     8821 cctcgttcca ttcgtagaac gggaagtcca cgatccaggc gaattcgaat cggttctcgt
     8881 cgatcaggtt cagctcgcgg ccaacccgat cccgggcgag accggcgaac ttgtagaagc
     8941 gcactggatc accggccacg aagaagcagg cgtcgcccac tttcaggccg agctgatcgc
     9001 ggattccgcc cgttcgttcc ggaccgatgt tcttggcgat cgggccggca ccgccttcct
     9061 cgccctcgcg ccagaagatg taaccaaggc ccggctggcc ctcgctttgc gcccaggagt
     9121 tcatgcggtc gcagaaggcg cggctgccgc cgctgggagc gggcaccgcc cagacgcgga
     9181 ccttcgggtc tttctcgatc atgccggcga agaccttgaa gcccgagcca cggaagtgct
     9241 cggtcacatc ctgcatctcg atcgggttgc gcaggtccgg cttgtcggtg ccgtacttgc
     9301 ggatcgcctc ctcgtaaggg atgcgcggga acacgggcgt gaccggcttg ccctcgccga
     9361 actcctcgaa cacgccgcgg atgatcggct ccatggtctg gaacacgtcc tcctgctcga
     9421 cgaagctcat ctccacgtcg agctggtaga actcaccagg taggcgatcg gcgcgcggat
     9481 cctcgtcgcg gaagcagggt gcgatctgga aatagcggtc gaagcccgag atcatcagca
     9541 gctgcttgta ctgctgcggg gcctgcggca gcgcatagaa cttccccgga tgaatgcggc
     9601 tcggcaccag gaagtcgcgc gcaccctcgg ggctcgaggc cgtcaggatc ggcgtcggga
     9661 actcgttgaa gccggcctcg tgcatgcgcc gccggatcga tcgcgtgatg ccgagccgct
     9721 tcatgatgat gccgtgcagt gtctcgcgcc tgagatccag gaagcggtag gtgaggcgca
     9781 ggtcctccgg gtagtccggc tcgccgaaga ccgggacggg cagctccttg gcctcggaaa
     9841 gcacctcgat ttcagtcgcg tagacctcga cctcgccagt cggcagctcg ggattgacgg
     9901 tgccctccgg ccgctcgcgg accgctccgt cgacacggat cacccactcg gagcggaccg
     9961 actcggcggc cttgaaggcc ttgctgtcgg ggtcggcaac gacctgggtc agtccgtaat
    10021 ggtcgcgcag gtcgatgaac agaagcccgc cgtggtcgcg gacgcggtgc acccagccgg
    10081 acaggcgcgc cgttttgccg acgtcggatt tgcgcagcgc gccacaggtg tgcgtccgat
    10141 agcggtgagt cggaatctca tgggcggctt cgccagccga tccgctgccc gaagggctcg
    10201 tcaccttgtc ctcagccatt tgaaacgtct tgcctgcgct gccgatgtcg ggaagtgacc
    10261 ggaaaagcgc acgtgaccga ggggttgtca agctggcgac ctgcagctga tcgcgaacag
    10321 ccatgcacgc tcgatcagcc agcgccggtc ccggtcgagg agcacggcct gccggtgccg
    10381 ctgctgcggc atacaaggga ggcgacaacc gtggcgtgct gggttatatc gcccgaccga
    10441 tgcgcgtcat tacgacaaat tccgacctcg cggaagcttg tgcccggctt gcccggcatc
    10501 ctttcgttgc aacagacacg gagtttcttc gcgagcagac gttctggccg gtgctctgcc
    10561 tcatccagat ggcaggcccc gaggacgagt acatcgtcga ccctcttgct ccggggctcg
    10621 acctcaagcc cttctacgac ctgatggcca acgagcgggt gacgaaggtc ttccacgccg
    10681 gccggcagga cattgagatc gtctatgcca gggccggcct gatcccgagc ccgattttcg
    10741 acacgcagat tgccgccatg gtttgcggct tcggagagtc ggtgagctac gtgaacctgg
    10801 tcaagaagct cacgaacaag gacgtcgaca agtcctctcg cttcacggac tggagccgcc
    10861 gtccgctcac ccagaagcag ctcgcctacg cactggcgga tgtcacgcat ctgcgggagg
    10921 tctacctcaa gctcaaggcg gagctggaga gcagcgggcg cgagcactgg ctcagcgagg
    10981 agatggcgac cctctccgac cccgcgacct atgagacgaa gccggagaac gcatggctgc
    11041 ggctcaagat gcggacacgg aaccgccgct cgctcggggt gctcatcgag ctggccgcct
    11101 ggcgcgagcg ggcagcccag atgcaggacg tcccccgctc ccgcatcctg cgcgacgagg
    11161 ccttgtacga tatcgccaac caggcgccga ccgacacggc aaagctcggc gagctcagaa
    11221 ccttgagcga gggcttttcc cgttccgcgc gggcgcggga gatcgtcgag gccgtgaagc
    11281 gtggcctcga gcgcgacccc aggacactcc cgcagatccc gacaggccag ccgctctcag
    11341 cggaagccac ggctctggtc gacctgctcc gcgtgctgct caaagccgct gcggcccgca
    11401 atcgggtcgc gccgaagctg atcgccgata ccagtgactt ggagcggatc gcctccgaag
    11461 acgatccgga cgtgccggca ctgcgcggct ggcgacggaa gctgttcggc gcggacgcgc
    11521 tgaagttgaa acgcggagag gtcgccttga ctgtcgcggg cggcgaggtg gttgccgtgc
    11581 ctacggccac ggcgaaagtc tagcccaggt cttaatatcg cgccgattgc tccctcgttg
    11641 ccgctcgaac gcgtttcctc atcaggaacc gtgaatgatc ttcaggtcgc gcatgactgc
    11701 gacagccaca tccttgttgt agttcgcgac cattcgagga atgcggaaca gatctataag
    11761 ccaccagatc aagccgacga ttccgaaagt aacaatggat gcaatccaca tgatgatggt
    11821 catcccccaa cggccaagat agccgtaatg gcaaaacaga agagagcaaa ggtaggcagc
    11881 gggcgtactt gccagctttc tcttgaactc ctcggcaaat tcggcctgcc tctcaggtgg
    11941 aagcttcgcc aactccgact tcaccacctg cgggagcttg tccaatagct cgggttcgat
    12001 caggagagac cgcgcccgca caggatcagt ggcggtcatt cgaatacctc aattcctgaa
    12061 actgctttca agcatcattc gcctctggaa ctctatcttg acggccttcg ttcttcggat
    12121 cgttaatctc cgatgcacat tattggttgc gcgcgcaacc tggagctctg acggctgatt
    12181 cggggaacca acttgtcgcc tacgctgttc gtggtctcga cgggacctcc ggacggagcg
    12241 tagcgcatgg ccacttcgct gcagcagcaa cattatcgcg cctaccagcc acgccccctc
    12301 accaaagccg aaatcggtaa tgtggagatt ctctatggcg gactgcattg gcgcgccgag
    12361 cacgtcatgc aggcggtgtt cgagaacatg ggctacaagg cccggccgct accggtggca
    12421 acacggaatg atcttttgct tggccgcgag ctggctgaca tcggacaatg ctgcccgacg
    12481 agctttacga ccggcaacct cgcaaacttc ctgcgcgagg aggccaagcg gatcggggca
    12541 gaagcggtgg ccgacaagta cgtgtacgtc accgcaggct cctgcggcgc ctgtcgcttc
    12601 ggccagtatc atcagagcta cgagctggcg ctgcgcaaca tcggcctcga ggcattccgc
    12661 atatttctgg tcgcgcagaa ccggatggat cagggcgcgc tcgagggggg cggcttcgag
    12721 gtcaacattc cgctcacact tggagtggtc tgggcgattc tgtgcacgga cgctttgcag
    12781 agcatggaat accggacgcg gccatacgag gtgatccccg gtcagacgga ccgcgtggtc
    12841 aaggagagcg tcgactacct atgcgaggtt ttccgaaacc gcccggtaaa aggcgagaag
    12901 tggggcccgc tcgcctggca tctgacgacg aactatttca tcaaggcgtt gcgcgaggtt
    12961 tatcgcaaat tcgacgccat cgaggtggac cgactgcagg tcaaaccggt ggtgaaaatc
    13021 accggcgaat tctatctgca gaccgtcgag ggcgatccca actacaatat ccatcgctgg
    13081 ctggaatccg aaggcgctga ggtttatccg gcagccattg tcgtctggct ggattactgg
    13141 atccggttct atgtccagga gctggaggat cgcattggca ccgaccgcac ggcgaaggcg
    13201 aagatctggg cgctgaaggg gttgcagaag gtcttccgcg gcacttacaa ccgcatgcgc
    13261 aaggcgctgg gcaacatccc gcacgaaatc ccggaccagt atgaactgcg gcagctggcg
    13321 gcgccctatt tccatcaccg gctttcgggc ggtgaggggg acatgctgat cggcaaggcg
    13381 ctgtgggcgc atcttcacaa caaggcgcac atgatctgcg agctgtcgcc ttactcctgc
    13441 atgccgaaca cgatgagcgt cggggcgatg gccggtgtcg taggccagca tcccgatctt
    13501 ctctatgcgc cgatcgaggt gaagggcgat gcggaggtgc atgcgctctc ccgctgtcag
    13561 atgatcctga ccgaggccaa gaagcgcgcc gtccgcgagt atgtgaaagc gatggagaag
    13621 acgggactcg atccggaggc tgtcaaaaaa gtgctcgaga ggaagcccga gatgcgcaag
    13681 gccacctatc acgtgcccca catgggcgcc gccgggacag ccgccaacac gatgctccac
    13741 gttgcagaca ttagttgaac tggtagcagc tatgacattt ttcacctctc ccataaggag
    13801 aggtcggccc gaagggccgg gtgaggggta caacgcctgc aattgtccga acccccctca
    13861 cccccggatc aggtccgggg caggctctaa ccctctcccc gccggggaga gggaatgggc
    13921 gtgaggttct tcgatcggaa atccgccagg caaggcaggt gacatgaaga cggtcggcat
    13981 ggatgtgggc tcgacgacga tcaaggcggt ggtcgtcgag gggggcgaag tgctctggcg
    14041 cgattaccag cgccacaaca ccaagcaggc tgaaaaggca ctcgagttcc tgattcgcat
    14101 ggagcaggag gttgggctga ctgagggccg agaccgcatc ttcttcaccg ggtccggagc
    14161 ggggctgatc gcgcctctgg tcggcggcaa gctcattcag gaagtcgtcg cagtagccgc
    14221 gtccgtggag aagatgcatc cggacgtgca cttcgtttcc gagatcggcg gggaggacat
    14281 gaagacgctg ttcttcaagg aatcgggcgc cggcaagtcc aagcagatct atatgcaatc
    14341 ggcttgcagc ggcggtacgg gcacgttcat cgagaagacc gcccgcaagc tgaggatccc
    14401 cgctgagcag ctctccacca tgccgtatga cggcatgagc ctgcacaagg tcagctccaa
    14461 gtgcgggatc ttcgcggagg ccgacgccaa cacgctgctg aagggcggag tgccggtcga
    14521 ggagatcatc gcgagcctgt tcgaggcggt cgtttaccag aacctcgcga cgctgaccaa
    14581 gggcaacacg cccctgccgc aggtgctgct gcttggtggg ccgaatctgt tcttcaaggg
    14641 tctgcagcag gcctggcggc atcacctgac gaacctttgg caagaccgga aatgcgagct
    14701 gccgccaggt gccgaccctg ctgatctgat ccatgtgcct gacgatgcgc tctattacgc
    14761 cgcgctaggc tgcgtcgagg tcgcgagcgg cgaagcggag ggcgtagggc tttatcaggg
    14821 gacggagcgg ctcagctggt ggatcgatga gggccagcat caggaaaagg cgaagagtgg
    14881 cgccaaggcg ctcattcgcg aaggtgacga cctcgctagc ttcaaggcag ccttcgaggc
    14941 gagccgcggg tccaccgctg cctcggcccg ctccagtgat ggccccgttt tcattggctg
    15001 cgacttcggc agcacgacag ccaaagccgt ggtgatggac tccgagaagg agatcctggc
    15061 cagcgtctat gtgcagtcca agggaaatcc gatcgaggat gcgaaagcgc tcttccggga
    15121 tattcgcgaa aaggctcgcg gttccgtcgc aggacttgcg ctcacgggct acgggaaaga
    15181 tcttttgaag gacatccttg gcgccgacac cggcgtggtc gagacggtgg cgcatgccac
    15241 ctcggccctg cacattttcc ccgatgcgga cgtgatttgc gatgtcggcg gcaccgacgt
    15301 gaaaatcatg atcctgcggc agggcactgt ttccgacttc cggctgaact ctcagtgttc
    15361 ctcgggcaat ggcgccttcc tgcagggcgt ggccgagcgc tacggcatcg cgctcgagga
    15421 atacgcggac aaggcattcc aggcgagggc catgccgggg ctcgcgatgg gctgcggggt
    15481 gttcctgcag tcggacatcg tcaatcagca gcgcaagggc tggtcggccg aagagatcat
    15541 ggcgtcgctc gcggccgtac tccccttgaa cgtctggatc tacgccggtc agctccagaa
    15601 cctgcgcgcc gtcggtcgga agttcgtgct gcagggcggc acgcaccgga acatggccgt
    15661 ggtgaaggcg cagatcgatt tcatcaaatc gaaagtgccg gatgccgaaa tcctgatcca
    15721 cccctattcg ggcgaggcgg gcgccatcgg cgcggcgctt tgtgcgatag acgctcacgt
    15781 cgaggggcgg ccgagccgct tccgcggctt cgatctcatc gagcagctcg aataccgcag
    15841 cacgaccagc gaggacacga tctgccgctg gtgcccggtg aactgcaagc gggccttcat
    15901 cgacgtgaag ctcgacggcg ccaaggggcg gccgtggagc aaggtgcccg tcgagcccgg
    15961 ctgggaacgc gtcatcagcg gcaactcgtg cccgaaaggc ttgatggagg acgccaacga
    16021 gatgcgggcg gtgaaggccc agatggagaa gcaacgccgt gaacttccca atattggcga
    16081 aatggttcgc gaacaggcct tccggcgggc atgggccgag gcctgaacgg gccgagcgcg
    16141 caaagctcag ggtcggtatt ccgaaggtcc tcaacatctg gacgacgcac caattttggc
    16201 tgggcttctt tgctagcttg ggcgtcagcc gtgtcgtgtt ttcatctgac acctcggaag
    16261 agcaggggcg cgagttcggc agcggccgcg gcacggtgga ctgctgctat ccggtgaagt
    16321 gcatttcagg ccactacggc gagctcatct tcggccagaa gcgcaagatc gacatcctgt
    16381 tctcgccgat gatctactcg atgccctcgt tcctgaaggg ccatgtcgct gactcgctcg
    16441 cctgcccgcg ggtgatggcg gcgccggaga acatcaaggc cgggtttctc aaggaaaagg
    16501 acgtctttgc cgagcagggc atccgctacg tggcgccgct cgtctcgctc gccgacccga
    16561 tgcaggtgcc gcggcagctc ttcaaggcct tcgacggcgt gctcacgggc ctcaccttcg
    16621 atgaggtgcg tgaggcggtg gatcaaggct accgcacgtt ggccggcttc gaccgcgaga
    16681 tgcgggcgcg gagccgaaag atcctggaat ggtgcgtacg cgagcagcgg ccctgcgtga
    16741 tggtgctggc gcgcccctac cacatggacg ccggcatcgg ccacgagatc gaggcggacc
    16801 tgcaggccta cggctatccg atcctgtgga tgcaatactt tccgaccgac cctgacctgc
    16861 tcgattggat gttcggcccc gaaatccgcg acggctacat caagtcggtg ctcgacatct
    16921 cggacgtctg gccctcctcc tacagcgcca acacgaacga gatcctgtgg ggcgcgaagg
    16981 cagcggcacg catgccttgg atcacctgcg tcatccgttt gacgagctat gaatgcggca
    17041 tggaccagcc gacgttcaca cccgtgcagc agatcgtcga acggtcgggt acgctcttct
    17101 tctcgtttca ggagctcgat tcgacgaagc cagccggctc ggtgaagatc cggatcgaaa
    17161 cgatcgcgca ctatctggaa cgcaattcgc ccaccatcat cgcgcgaaag ctcgctgcca
    17221 tgccggaggg atgcccgctc ctgccgagcg cagcgccagc ctagcaaagc ggcgtacgac
    17281 gcccctgtgt ttccactagc ggattgcttc ctcccctaca gcggcgcctt gtgaccaccc
    17341 gctttttcgt ttagacggct gcaaacactc cagaccagcg gtcgcgcccc ccgtgtcttg
    17401 gattcgcgcg gggcgcttct gcgaaagtga tgtgatggaa catccaggat ttttcgagcg
    17461 cgccggcccg tttccattgg ccgaggtcgc ccgcgccgcc gcagccgagc tcgcgccggg
    17521 cgccgatggc gacgccctga tcagcgacgt gcgcagcctt gccgacgccg gcagagatca
    17581 cgtttcgttc ctcgacaacc gcaagtatct tccccagctt tcagccacgc gggctggcgc
    17641 gtgcctggta tcacctgctt tcgccgcgcg ggtgcccgag gggacggccg cgttgctgac
    17701 caaaacgcct tatcgcggct ttgcactggc cttgcagctc ttctatccgg atgcgcttcg
    17761 ccccaaggtg gcgcgtccag gcggcaccag gctcgtcgat cccagcgccc agctcgagga
    17821 tggcgttgtc atagagccgg gggccatcat cgggccggag gctatgattg gccgcggcac
    17881 cgtcgtcgct gcgggcgccg tcatcggcta tcggtgtgcc atcggccgcg actgcttcat
    17941 cggccccaac gcgagcgtga cgcacgcgct ggtgggcaac cgcgtcatcc tgcacgcggg
    18001 cgtgcgcatc ggccaggatg gcttcggctt cgccatggga ccgcaaggcc acctcaaggt
    18061 tccgcagatc gggcgggtga tcatccagga tgacgtggaa atcggcgcca acaccgccat
    18121 agaccgcggc gcgctcaacg acaccatcat cggcgaaggc acgaaaattg ataatctcgt
    18181 gcagataggg cacaacgtcg tgatcgggcg gcactgcgtc atcgtagggc actgcgggat
    18241 ctccggttcg acggagctcg gcgacttcgt ggtgatgggc ggccagtccg ggacggtggg
    18301 tcatatcagg atcggcacgg gtgcacaggt ggctggcgcc tcgcatccga aggaggacgt
    18361 accagcgggt gcgcgcgtcg gcggcacgcc agcgcgtcct ctcaaggagt gggcgcgtga
    18421 gctcgcggtc ttgagccggc tcggcaaaca gggctatgtc acgcccggaa cggacggtga
    18481 tgagggcgga aactagggga tggaaggact agtcatttct caggagagcg ggggagacgg
    18541 agtgcaggac aatgaatgac gcgacagcaa aggagcagct cggcacagcc gatatcgcgc
    18601 gtctgctcaa gctgctgccg catcgctatc cgttcctcct ggtcgatcgc atcttcgaca
    18661 tggatcgcga cgagtccgcc gtcgggctca agaacgtgac catcaacgag ccattcttcc
    18721 agggccactt cccgagctac cccgtcatgc cgggcgttct catcatcgag ggcctcgcgc
    18781 agacggcggg tgcgctgtgc gtccatagcc tcggcgagag ctaccggccg cagctcgtct
    18841 acttcatggg aatcgacaag gcgaagttcc gcaggcccgt cgtgcccgga gaccagctct
    18901 tcttccacgt ccgcaagatc cgcagccgtg gccgcgcctg gcgcttctat ggcgaggcga
    18961 aggtcgaggg gcaggtcgcg gcagaggcgg agatcagcgc catgatcgtc gatacgcccg
    19021 agacccaggc gattgcgagc cagaatggct gacgcacaga tccatcctac ggccattgtc
    19081 gaatcggggg ccaagctggg cgccggcgtg aagatcgggc cttactgcat ggtcggcccc
    19141 gaggtcacgc tgggggacgg ctgcgagctc atcagccacg ctgtcgtcgc ggggcggacg
    19201 acgatcggtc cgcgcacccg catcttcccg ttcgcgtcca ttggccatca gccgcaggac
    19261 ctcaagtaca ggggcgaacc ctcgacgctg acgatcggtg ccgactgcat catccgcgaa
    19321 ggcgtgacga tgaacccggg caccgaaggc ggcggtctcg agactgtcgt gggcaacggc
    19381 tgcgcttttc tcgccaactc ccacgtcggc cacgactgcc acgtcggcaa ccacgtgatc
    19441 ttctcgaaca acgtgatgct ggcgggccac tgcaccatcg gcgactacgc catcctcggc
    19501 ggcggcgcgg cggtgatcca gttcgcgcgg gtcggcgcgc attcgttcct cggcggcatg
    19561 tcggggctcg agaacgacct cattccctat ggcatggcgc tcggcaatcg ggcgtacctc
    19621 tcgggcctca atatcgtggg actgcagcgg cgcggtttct cccgggaaac catccacgat
    19681 ttgcggcgcg cgtatcggct cctgttcgcc gccgagggca cgctgaaaga gcgcgtcgac
    19741 gatgtcgccg ccgagttcgc ggaccacgcc ctcgttcagg agatcgtggc gttcatccgt
    19801 gccgggggca agcggtcgtt gtgcacgccg cgcgactccg agggctgatg gtgagcgcgg
    19861 gcgacggtcc gctcggcatt ctggccggga acggcgcact gcctctcgaa gtcgccgatg
    19921 aagtgtcccg caatggcagg cccgtgcaca tcgtcgccat cgccggagag gccgagcccg
    19981 gtatcgagcg gcatccgcac acctgggtcg gcttggggca gatcggcggg ctgctacgcg
    20041 ccttccgcgg agcgggctgc cgagacatag tcattcttgg cggcgtgcgg cgaccggacc
    20101 tgaagaggct gcgcgccgac ctgggctttg tcatcaacct gccgctcatt ttgagcctca
    20161 tggccggtgg cgacgacagc gtgctccgca agatcgtttc cttcttcgag tggcgaggat
    20221 tccgggtggt cggggctcat gacgtggcac cgggtctcct ggcgaaggat ggtccgctcg
    20281 gcaaccggaa gcctgatgat cgggtgagcc gtcatattgc gttgggcttt gccctcgtcg
    20341 aagcgcttgg accgctcgac gtggggcagg gggtcgtgat cgctggcggc aggcccgtcg
    20401 ccatcgaagc ggccgaaggc acggacagga tgctgatgag gctcgagagc ctccgtgcca
    20461 gcggcatcac ttcgggcgga gttctcgtca aggcaccaaa gccgggccag gagctgcgga
    20521 tcgatttgcc cgccgtgggc ccgaagacgg tcgagctggc atctgcagcc gggttggcag
    20581 gcatcgccgt tgcggccggg caggtgcttg tggccgagcg cgaccggctc gcgcagcggc
    20641 tcgccgagac cggcttgttt ctgatcggca gcagaacgaa gcttcccgat agcgtacctg
    20701 cgaacgatga ggcggccgcc gcctcgcggt ggggtcggct ccgctggatc accggaaaag
    20761 tgaggtcgcg cgatattcgc gatgcagagc gagcgctcca ggccatatcg tcagcccgcc
    20821 cattcggggc aggggcagct gctgtttccg tccgcgagca cgtgctggcg ctcgaggccg
    20881 gcgagggcgc tgaggccatg ctggggcgcg tcgcaggtct gaggcagtgg ggcgaccggc
    20941 tttccggcca ccgccgcggc gttgctgccc tgcagccggt cgatgccgat cccgcgcatc
    21001 tcgtccagct tgccgatacg gctggcctgg ccggtctggt gatggcaggc gcgtggaccg
    21061 gccgggctca tctggagctg ctggcagcgg catcaaggcg ccggcttttc gttctggctt
    21121 gcgaggacag cggatgaacg acggcgagga tccgctgcgg gttttcctgg ttgccggcga
    21181 gcaatcggga gatgccctcg gcgcgcggtt gatgggcgcc cttcaggtaa agctcggtga
    21241 tcgcgtcacg ttctcaggcg tgggcgggcc gctcatgggg aaggaggggc tcgagacgct
    21301 gttcccgctc gccgatgtgg cggtcatggg tccgttgtcg atcctgccgc ggctgccgcg
    21361 catcatcagg cgcgttcatc ggaccgtcga tgctgccatc gccatgcggc cggacgtggt
    21421 ggtgatcatc gacagccctg agttcacgca cgccattgcg cggcggatcc gccgccgctg
    21481 cccgtcactg ccgatcatcg actacgtcag cccatcggtg tgggcatggc ggcccgggcg
    21541 cgcgaagcgc atgcggcggt atgtcgacca cgtgctggcg ctgctgccgt tcgagccgga
    21601 ggcacacctg cggctgggcg gaccgccgtg cacctatgtg ggccatcctc ttgtcgagcg
    21661 gcgcgcatgg ctggaggccc tcgatcctgc tccgctcgct gaaaggctca cactcgatcc
    21721 ggaaaggccg gtgctgctag ttctgccggg cagccggtcg tcggaggtgg gtcgtctgat
    21781 gaaaccgttc ggtgaggcga ttgggctgct ggtcggccag ggagcgcgcc cccagaccat
    21841 tatcccggcc gttccgcatg tccgcagtca gatcgaagta gcactcgaca gctggccggt
    21901 tcgcccgcat ctcatcgccg gcgaagagga caagttccgg gccttcaagc tcgccacggc
    21961 ggcgttggcc gcgtccggca cggtgacact cgaactggcg ctgacgggca cgccggcggt
    22021 ggttgcctac aaggtggatg cgattgccgc gcgcctgcgc tttctcgtga aagtaccgtc
    22081 ggtcgtgctg gcaaacctgg tgctcggaga gaatgtctat ccggagttca tccaagaaga
    22141 ctgcagtccc gagaaactgg ccgcggcctt ggcaccgctc ctcggcgata caccggagcg
    22201 ctggcgccag cgcgatctgc tcgcccgcgt ccccgaacgg ctcgccgttt cggccgcaag
    22261 ccccagcgaa gcggccgcag agatcgttat ccgccgcggg agggctgctc gccctcggcg
    22321 agggtaactg atctgaaatc aaagctgcag ttcagccccg attctcgatc ggcacatagt
    22381 cgcggcgggt ggcgccgatg tagagctggc gcggccggcc gatcttctgc tcgggatcct
    22441 cgatcatctc cttccactgc gcgatccagc cgaccgtccg tgcgacagca aaaagcacgg
    22501 tgaacatcga tgtcgggaac ccgagggcgc gcagcgtgat gccggagtag aaatcgatgt
    22561 tcgggtaaag cttcttctcg atgaagtaat catcctcgag cgcgatgcgc tccagctcca
    22621 tcgccacctt caggagcggc tcgttcttca tgccgagtgc atccagcacc tcgtggcagg
    22681 tcttctgcat cacccgagcg cgcgggtcat agtgcttgta gacacggtgg ccgaagccca
    22741 tgagccggaa gccggagctc ttgtccttcg ccttggcgat gtactcgggg atgcgatcga
    22801 cggtgccgat ctcctccagc atgttgagcg ccgcctcgtt ggcgccgccg tgtgcggggc
    22861 cccacaggca ggcgatgccc gcggcgatgc aggcgaacgg gttggcgccc gacgagccgg
    22921 ccagccgtac cgttgatgtc gatgcgttct gctcgtgatc ggcgtgcagg atgaaaatgc
    22981 gatcgagcgc acgggcgagc accggatctg cgaaataagg ctcgcaaggc accgcaaaac
    23041 acatctggag aaagttcgcg gcgtaatcga gatcgttgcg cgggtagatg aacggctgcc
    23101 cgatcgaata cttgtaggcc atggccgcaa tggtcggcat cttggcgatc atgcgatgcg
    23161 aggcgatcat ccgctgcaca ggatcgctga tgtcggtgga gtcgtgatag aaggccgaca
    23221 gtgcgccgac ggtgccaacc atgatcgcca tcgggtgcgc gtcgcggcgg aacccagtga
    23281 agaaccgggt catctgctcg tgcaccatcg tgtgacgggt gatggtatcg cggaaccagt
    23341 cgagctgctc tctcgtcggc aactcgccgt aaagcagcag atggcagacc tcgataaagc
    23401 tcgattgctc cgccagctgc tcgatcgggt agccgcgata gagcagcaca ccctcctcgc
    23461 cgtcgatata ggtgatcttc gaggagcagt tggcagtgga cgtgaagccg gggtcgtaag
    23521 tgaagcagcc tgtttctcgg tagaggcgcg tgacgtcgat cacatcaggc ccaatcgacc
    23581 cggcccggac cggcaggctc acttgtttgc cgttctggtt cagggtcaca gttcttgtgg
    23641 catcggcctt gcccgacgcg ctgtcgtgca cgttcatcgc caatcctttc gatcgtctcg
    23701 acgcctgccc cttcggcgcc aagtctggag ggacccggcg cgcaatggcc gcccggcgcc
    23761 cattgcctat tacacatttc gcgctgcgac aagacagcta atggacgccg ggccaggaac
    23821 cgcaaaccga gcgacgaaaa tcaacgccgc accggtgcag gcggtttcca acaagagttg
    23881 ggagcggtga cgcgcgagtt tcaggcgctt gcctggtcgc ggatgcgcgc gagggcttcc
    23941 tcgcgaccga gcacggccat gacgtcgaag accgggggag agacggtacg gccggtcagc
    24001 gccgccctga gcggttgcgc gatgccgcca agcttcacgc cttccctctc ggcaaagctt
    24061 cgcacctccg attcgagggc gccggctgtc cattcggcgg attccagctg cggcagcaag
    24121 cggccgagca gcacccgggc ctcatccgtc aggagttttg cagctttctc atcaaggacg
    24181 agcggcctct cggcgatgag gtagccggcg ccgtcaacca gatccttcaa ggtcttggcg
    24241 cgcggcttaa gacttggcag cgcagctgcg agcttctccc acccggctgc ggcgaagcgc
    24301 gcctcaagct gaggaccgtt ctcgaattcc ggcagggctt gccttatgcg ggccagcaat
    24361 tcatcgtccg gcgtattgcg gatgtaatgg ccgttgaggt cctcgagctt ggcaaaatcg
    24421 aagcgcgcag gcgagcgccc gatgccgtca agatcgaacc agtcgatcat ctgctgagtg
    24481 gagaagatct cgtcgttgcc gtggctccac ccgagtcgga caagatagtt ccgcagggcc
    24541 gcggggagat agcccatggc gcgataggct tcgacgccga gcgcgccatg gcgcttggag
    24601 agcttggctc cgtcgggccc gtggatcagg ggcacgtgtg cgaagaccgg cacgtcccaa
    24661 tccattgcat tgtagatctg cgtttgccgc gcggcgttgg tgaggtgatc caccccgcgg
    24721 atcacgtggg tgatccccat gtcatgatcg tcgacgacga cggcgagatt gtacgtcggc
    24781 gtgccatcgc tgcgcaggat gatgaggtcg tccaggttct cgttctggaa aacgacgcgg
    24841 ccctggacct gatccacgat gaccgtttcg ccggtccgcg gcgtctttag ccggattgcg
    24901 ggcttcacac cgggaggcgc ctcggacggg tcgcgatcgc gccaatagcc attgtagacg
    24961 ggcggccggc ccgcggcctc ggcctccttg cgcatggcct cgagctcctc cggcgtgcag
    25021 tagcagcgat aggcattccc ccgccgcagc agctcttcga cgacctcccg gtgccggtcg
    25081 gcgcgcgtcg actggaacag cggctcgccg tcccaatcga gctccagcca cttcaacccg
    25141 tcgaggatgg cggcgatggc ctccttggtg gaacgctcgc gatcggtgtc ctcgatgcgc
    25201 aggaggaagg tgccgcccgt gtgcttggcg tagagccagt tgaaaagagc ggtacgcgcg
    25261 ccgccgatgt gcaggaagcc cgtcggcgat ggcgcaaagc gcgtgacgac cttggaccct
    25321 tggcttgtcc cggtgctcat gatttgcgtt agtctcgcgt gccggatgtt gaacgtgcgc
    25381 cgcggtttag cacagccggg gacacggagc gagcggggcg gggtcacttc gtgagcttgg
    25441 gggagggaac ggttgacgcg gtccgccgcg cgcgggagcg atcgcgtgcc ctggcccccg
    25501 gacgatggct cctggatcga ctcctggccg agcaggaccg ctggttcctt tggacacccg
    25561 tgctcttcgg gctcggaatc ctcatctact ttcgattgcc tgccgagccg cagatcgtcg
    25621 ctgcgctggc tccagtcatg tgcgtcttcg cattggcccc gatggccagg cgtgccacgg
    25681 ccggactgct ggccatcaac gcggccattg ccgtatcgct cgggttcgcc gcggccaaga
    25741 tgagagcgga gtgggtcgct gccccggtgc tcgaacggca gctctacaat tctggagtcg
    25801 agggcttcgt ggaggtcgtc gagccgcggc ccacccgggg gcagcgcatc acggtgcgcg
    25861 tgagccgcct tggaaagctt ccgccggaca agcgaccgca ccgggtcagg atacggttga
    25921 tgagtgagat gccgggtctg gagcccgggc aagccgtccg cctcaaggcc acgctcgcgc
    25981 caccctccat gccggccctg cccggcgact acgacttcgc tcgcgcagca tggttttcgg
    26041 gacttggcgg ggtcggctac tcgatggcgc gacccgagat tatcgcggat gccggcgaag
    26101 cgccgtggtc gctgaagctc tacgcgatga cgcagcggct gcggaacgag attggccggc
    26161 gcatcctggc ggcgttgccc ggcgaggaag gggccatcgc gacggcgctc atcaccggag
    26221 agcggggcag catctcaccc gaaaccacag cagcgttccg tgattccggg ctggtgcaca
    26281 ttctgtccat ctccggcctg cacatggtca tgatggcagg gtccgtgttc ttttcggtcc
    26341 ggctgttcct ggcagctatt ccagcgatcg cgctgcgcta tccgatcaag aagtgggcgg
    26401 ccgcggcggc gatcctgggt gccctcggct acctgctgat atcgggcaac tcgtttccga
    26461 cgatccgggc ctggatcacc atctcgatcg tgttcggcgc catcatgctc gatcgcccgg
    26521 cgcttgcact caggaacgtc gcgctggcgg cactcgccgt gctgatcctg gttccggaga
    26581 gcctattcga tgccgggttc cagatgtcct tcgcggccgt ggtggcgctc gtcgcggcct
    26641 acgagctgat ccgcgaccgc ctcgagcggc ggcgcagcga gccggggccg atgttcggtc
    26701 ccgtactggg attcttcatg tttttcggcg ggatcattct gacgacggtg atcgccagtc
    26761 tcgccgtcgc gccgttctcg gcttaccact tccacaccag ccagcaatac gcggtgctgg
    26821 cgaacctgat cgcggtcccg atctgcaaca tcgtcgtcat gccgggcgcg ctggcaacgc
    26881 tgctgttcat gccgatcggg ctcgaagcag taccgttgtg ggtgatggag cagggcatcc
    26941 gcgccatgct ctggtgcgcc cgcgccgtct ctcagctccc cggcgccgtc ggccatatcc
    27001 ccgcattctc ggacatggcc ttcgggctga tgctgctcgg aggcctttgg ctgtgcctgt
    27061 ggggaacgcg atggcgcttc ctgggcttag gggtgttcgc agcagggctt gcgcttgctc
    27121 cgatgatgcc gcggcctgac attctcatcg ggaacgacgc cgaactcgtc gcgattcggg
    27181 gcgtcgatgg caagctctcc gccttgccgg cgcgcgggtc gagctatgag ctgcagcgtt
    27241 ggctcgagaa tgacggggat tcccgcacgg cgcggcaggc ggcatctggt gaagggttcc
    27301 gctgcgactc ggtaggctgc cttgcgatcc tcaaaggaaa gcgggtcgcg gtcgtgcatc
    27361 atccagcggc gctcgccgac gactgccgga tggccgacgt gctgatcgtc ccgtttccca
    27421 aaccgagagg gtgtaatcca ccgggactgg cgatcgactt ctatgccgtg cgcgatcgcg
    27481 gcacgcacgc cgtctacatc gcggacggcc acctttccct ggaaaccgtc gccgatgcgc
    27541 gcggcgacag gccatggtcg cggcgaccgg agcggccacc tcggaagaca cgatccgagg
    27601 cggggaacac catttcacgg ctgaagggct tcgcggcgcc gcgagatctg gccgaggcgt
    27661 cacgccgaat gcgcgccgag ctcgaggacg acgacgatcc cgtggtcagg cgactggagg
    27721 aggaaggagc tgaattggac gatacgccat gagccgatga aataacgtca tttccgcgaa
    27781 ggcgggaatc cgtgatgcgc ttcagcaatc acgcgggtcc aatgcaagcg caggcgattg
    27841 ccctttggcg atgcatcgcg gatgccctac cgcccgcatg acggggcaag atgtaaccaa
    27901 ctcgacttac tttcctgaaa ccggatgtcc tcgggcgcgg aaagaggaac tcgccgcgcg
    27961 caatcccctg aagtccccgg ggaatcgctg aacccgaatc cgatcaatac cgccggataa
    28021 gccccaccaa tcggccttga atatcgatct ggtccggccc gaaaatgcgc gtctcgtatt
    28081 ttgggttggc ggcctcgagg gcgatggccg agccgcgctt gcgcaagcgc ttcagggttg
    28141 cttcttcctt ctcgaccagc gccacgacga tatcgccgtt ctcggccgtc tcgcagcgcc
    28201 ggatgatgac gatgtcgccg tcctgtatgc ccgcatcgat catcgagtca ccgcgaacct
    28261 ccagcgcgaa atgctcgcca ttggtcagca ggtcgggcgg gcaggcgatg tcgtgtgtgt
    28321 gggattgaat ggcactgatc ggcacgccgg cggcgatgcg gcccatgaca ggcaccgaga
    28381 tggtgcggct gtcgaccatg ctcgagggcg gcaccgagag ctgggactgc ttgccaaggc
    28441 tgccctcgat gacgctcggc gtgaaaccgg agcgccgcgg ctgtgtcggc tccgaattct
    28501 cgggcagctt gatgacctcc agcgcgcggg cgcggtgcgg caggcggcgg atgaagccgc
    28561 gctcctcgag cgccgtgatc aggcggtgaa tgccggactt ggacttcaac gccagcgcat
    28621 ccttcatctc gtcgaaggag ggcggaaccc cggtttcctg cagacggccg tggataaaga
    28681 gaagcagctc tttctgtttc tgtgtcagca tctgccccgg gcccctcggc aaggaatacg
    28741 cgcgactggt gtcgcctgta caaatcatga acagaatact cgttccggac ttgttctgca
    28801 agctgttcgt cgccggcggt tgagaatgtg atcagaagtc gagaagaagg acggtcactt
    28861 cggtgcccgc cggcaggggc ggagcatttg gcggcctgac gagaagcacg ttgctggtcg
    28921 ccagcgtggc gagcagcgag ctgtcctgtg agcgcgcaag ccggacttgc tggcggccat
    28981 ccggcaggcg ctcgaggatg gcgcgcatat aatgctggcg ggcgccgttg gcctccaccg
    29041 gctcggcgag gggtaccgtc accgtcgccg cgtcctcctg cggcaggccg agcagggcgc
    29101 gcacgagcgg caccagaaac acgcgggtgc agatcagcgc tgaaaccgga ttgcccggca
    29161 atcccaggac acgctggcgc ccgagccgcc cgtgcagcag gggcttgcct ggccgcatgt
    29221 tgatcttcca gaacgccagc tccatgccga ggctttgcag caccggtccg acgagatcat
    29281 ggtcgcccac ggatgcgccg ccgatcgtga cgagcacatc cgcatcacgc gcggcctcga
    29341 cgtgctccac gaggctgtcg cgcgtgtcgc gggcaatgcc gagcaccttt gcttccgctc
    29401 cggcctgggt aagcagggcc gcgaccccca gcgggttgga ggagatgatc tgacctggcc
    29461 ctggtcgctc acctggccgc acgagctcgt cgccggtggc gagcacggca atgcgcggcc
    29521 tccgccgtac ggacacttcc ccgtagccgg ccgctgcagc cagcgtgacc tcccgcggac
    29581 cgagccggcg gccagggaaa aggagcgcct ccgactcgcg gaagtcgaag ccgcgcggac
    29641 ggatgtgcgc ccgatcgggc cggccctcac gcacctcgac ggtgccgtcc gttgcgctcg
    29701 tattctcctg aataacgacg gcgtcggcgc ccttcggcag cggagcaccc gtgaaaatgc
    29761 gcacagcctg accgggctct atctcgccgt cgaacgggtg gcccgctgcc gcctgcccga
    29821 tcaccttcag tgttgccggc aattgcgcga catcggcgct gcggacggcg tagccgtcca
    29881 tggcggatgc atcgaagggc ggctgcgtca ggcgcgcgat cagcggctcg gcgaggatgc
    29941 ggcccgcagc ctgctcgatg ggaacgtatt ccggagaggt tggagcgacg tcagcgagaa
    30001 ctttctccag cgcctccgat acggacagag ccatgcgggt caactcccct gcgcatcatc
    30061 ggcccggaag tggccggacc ggccgccggt cttctccagg aggcggatgc cggagaaggt
    30121 catgccctta tcggcagcct ttagcatatc gtaaatggtg aggcaggcga cggaaacggc
    30181 cgtcagcgcc tccatctcga cgccggtctg gccggtaacc ttgacctcgg cggtcacccg
    30241 gatcccggtc ggctgatgtg aaagctcgaa atcgacggtt gccttggtga tcgggagggg
    30301 atggcaaaga gggatcagct catgagtcct cttcgcggcc atgatgccgg cgatgcgcgc
    30361 cgtcgccagg acatcgccct tcttggcttg gcccgtctcg accagcgcca gcgtctccgg
    30421 ctgcatagac acgaagcctt cggcgcgcgc ggtgcgctgg gtcacggact tgtccgacac
    30481 gtcgaccatg cgcgcttcgc ccttctcgtt gagatgggtg agcgggccgc aattcatgat
    30541 gtcctctctt gctcctccgg gaggcccagc agcttgcgcg tggctgacgt cacgtcagct
    30601 tggcgcatca ggctctctcc gacaaggaac gtcctcacgc ccaccgcggc aaggcgggtg
    30661 aggtcggcat gagtgaagat gccgctctcg ccgatgacga tgcggtcggc aggaacccgc
    30721 ggcgccagcc gctccgtcgt ggtgagagtc gtctcgaatg tcttgaggtt gcggttgttg
    30781 atgccgatga ggcggcagtc gagctgcagg gcgcgctcca gctccgcttc gtcgtggact
    30841 tcggcaatcg catccatccc ccactctcgg gcggccgcgg cgagatcccg cgcagtggga
    30901 tcatccactt gcgccaggat gatcagaatg caatcggcac cccaggcgcg ggcttccgca
    30961 acctgatagg gatcaagcat gaaatccttg cgcagggcgg gcaaattcgt gctcgcgcgc
    31021 gcagtggtga gataggccgg tgcaccctgg aaggacggcc catcggtcag caccgagagg
    31081 caggtggcgc caccggcctc ataggcgcga gcgagctccg acggctcgaa gtccgcgcgg
    31141 atcagccctt tggacgggct tgccttcttg atttccgcaa cgagcgcgaa gcggccgccg
    31201 gcgagtgtct tttcgatcgc gcccgcgaac ggccggaccg gagccgcggc gcgggccgct
    31261 gcctcgacct cggcgagcgg atgactggcc ttggcggcgc ggatctcctc gagcttgtac
    31321 cgagtgatcc tgtcaaggat gcccgtcatc gtgagccggt cagtcccgcc cgttggtgat
    31381 ggcgatcagg cggtcgaggg cgtgttgggc ggcgccgctg tcgatcgagc gcgccgcaag
    31441 ctcgaccccc tcggtgagcg actgggtctt gccgccgacg atcagcgccg ctgccgcgtt
    31501 gagcagcacg acgtccctga atggcccagg cttgcccgcg agcacgtccc ggatggcggc
    31561 ggcattcgcc tcggcattac cgcccttgag gtcagccagc ttggccggtg gcaatccggc
    31621 atctgcgggc gtcacctcga agagacggat cttgccgtcc ttgagctcgg ccaccttggt
    31681 cgagcccgtc gtcgtcagct cgtcgaggcc gtcgtggccg tggacgaccc agacgtgctc
    31741 ggagccgagg ttcttcagga cattggcgat cggctcgacc cattgcgcgt cgaatacgcc
    31801 gaccacctga cgccgcactc cggcaggatt ggaaatcggt cccagcaggt tgaagatgtt
    31861 gcggatgccg agctcggccc ggaccggggc ccagtgcttc atggccgcat ggtgcatcgg
    31921 cgcccacatg aacccgaccc cggccttcat gatgcagtcc gtgatgacgg gcggcaggac
    31981 gtcaagcttg acgccgagcg ccgccagcac gtccgaggcg cctgaaaccg aggagacggc
    32041 gcggttgccg tgcttgccga cgcgcacgcc ggcccctgcg gccaccagcg aggcgcaggt
    32101 cgagacattg taggtgccga ggccgtcgcc gccggtgccg acgatatcca ctgtgtccgg
    32161 cggggcctcg accggcgtca tcttcgagcg catcagctcg gcggcacccg tgatctcgtc
    32221 gacggtttcg ccacgcacac gcagcgccat caggaaggcg cccatctggg caggggaggc
    32281 gagtcccgcc gtcatggtct cgagcgcgga gcggatctcc gcggccgaaa gggtggctcc
    32341 ggttgcaacc ttgtggatca attgcctcag ttcgttcgtg ggcatctttg tgtactctgg
    32401 ctctcaagct ttcagaagat taggcggctt tcaaggatgg gcgcgtattg cgccatcgcg
    32461 gatcgaggcc ggcgatcttc aggaagttgg cgagcagatg atgcccgcat tccgaggcga
    32521 tgctttcggg gtggaactgc acgccgtgca cgggaaggga cgtgtgctgc aggcccatga
    32581 tgataccgtc gctggtctct gcggtgatct cgaggcagga gggcaggctc gcccgctcca
    32641 gcatgagcga gtgatagcgc gtcaccttga accgcttcgg cagcccctcg aacacgccct
    32701 tcccggtatg gcggagctcg gacagcttgc cgtgcatcgg cagtggcgca cgtaccacct
    32761 taccgccgaa agcctgaccg accgcctgat gtccgaggca gacaccaagg accggcacgc
    32821 tggcttcggc ggcagccgcg atcaggtcga gacagatgcc ggcctcgttc ggcgtgcacg
    32881 ggcccggtga aagcacgatg cccttcggct tcgaccgcag cacctcgtcg accgtgatct
    32941 tgtcgttccg gtggacgtcg caagccgcgc cgagctcacc caggaagtgg acgagattgt
    33001 aggcaaagct gtcgtagtta tcgatgagga cgagcatggt gaacgtgtag gggacgcggg
    33061 ccgattgcgt caagcgaggc tgcgtggcgc cacgaaccgg tcgaggtgcc ctgcagcggg
    33121 gtggacgtcg tgccagttgt cggtctcgaa agtcagcact gcaagagcac acgtcggaaa
    33181 tttcacggcc atggacgcga tgtcgcggcg caggcccctg ccggtgagat cgagggcaag
    33241 cccgtgcagg ccgggattgt gggccagcag caagagatgg cggacgctcg cgggcgtctg
    33301 gcggacgacg tgcaacatgc gggtagccgg ggcgagatag agtgcgggct cgatctcgac
    33361 cttcggcttg tggctcccaa gctttggcag gatgcagtcg agcgtctccc gggcgcgcag
    33421 cgcgctcgag cagaggatca ggtcgggtat gagatcgtga ctggcgatat acgcgcccac
    33481 gtctgccgct gcgcgcctgc caatggcggc gagagggcga tcgtgatcac tgagatcaga
    33541 gtcctcccac gacgacttgc catggcgaag gagcgacagc gtcaccatcc gatatcaccg
    33601 ggcgctgaga tcgattggat gaaaatagca cgatctggcc ccggtggcgt gcgagatcgc
    33661 gcggttcgcg actccgcaga gtactgatgg caggcgccca gcgctgagct atattcggcg
    33721 cccgctgtgc agcctcacga tcatcagccg aaatggtccg ctggccgcgc gtgcagtcct
    33781 ggggggaacg tcatggctct gcaactcgcc cgctggtccg tcatcgttct ttccgttgtt
    33841 acggcggcgc tcggtgctgt cctgggcatc ctgcgggccg ttgagccgat tcccgatggc
    33901 cgcatcgtgc tcgcgacggg cggctctgcc gggatctacc atgagctcgc ggaaagctac
    33961 cgtcgggagc ttgctcgcaa cggcgtgaag ctcgagctgc ggcagacctc ggaaggcttt
    34021 gcgacgctgc ggggacttct cgacaggggg tcggggctcg atgccggctt cgtgaaaggg
    34081 ggactggtgg gcagcctgca gggccgtctc gccagtgagc gcgcgcgcga ctggcacgag
    34141 cgggagctgg gcaagctccg ctccgtcggc cgaatgttct acgagccgat ctgggtcttc
    34201 acacgtggcg acctgcccat cgagagcctg cgggagctcg aggggcgaag gatcctggtc
    34261 ggcacccgcg gcagcggcgc tctgcgtgtc gtgcgtcagc tgctcaaggc caacggtgtc
    34321 gatgagcaga actccacgtt catccgggag gagttgcccg aggatgctgg gccgctgcta
    34381 agcggccagg ccgatgcagc cttgttcatc ctgccggccg acagcgagaa ggtgcagaag
    34441 ctgctgcggg ttcccaagat ccgactcatg aacttcgagc cggaagccaa ggcctatacg
    34501 aaccggttcc cggccctcag caaggtcgtc ctgcgtcgcg gcggggtcga gttcgatccg
    34561 ctgatcccgt cggcagacat tacgctgctg tcgacatccg ctgcgctggt catccgcgcc
    34621 gacctgcacc cggcgctcgt cagcctgctc acgcatgccg tgatccagaa cccgcggctc
    34681 gggttcgaca aggccggcga tccggtgctg ttcttccggc ccggcgagtt cccctcgccc
    34741 gacgacccgg agttcgaggt ggcggcggag gcgcgtcagg tctacaaaag cggtgggctg
    34801 ccattgctgc tcggcgttct ggccccgctc aacagtcggc tcgggctgcc gttcttcttc
    34861 accgctttcg gcgcatcgca tgggctcgag acgctgctgt tgatcatccc cattctgacg
    34921 atcctggtcc cgatcatgaa ggcgctgccg gtcatctacc ggtggaccat gcggcggcgg
    34981 ctcctctact ggtaccgcca gctcaaggcc ttggagcgga acctcgagga tgcccggtca
    35041 caggcggatt tcgcggtgac ccaggccgag ctcgagcgca tcgacgcgcg cgtgcggcgc
    35101 atccgcgtcc cgctcggctt ctccgaccag ttctatgact tgcggggtca catcgatctc
    35161 gtccgacaac gcatcggcca gcgcgctccg gccctgcgga tggcagcgga gtagggtgcc
    35221 cggtggggtg gactttggga gatccgaaga agggtcagcg gcggggagga ccgaggcggg
    35281 caggccggca cggacgtgcc cacgttttgc agacatagct cttgcccgag tttgagcact
    35341 ccatcctctt tcctgccgcg agccgatagc ttccccacgg acggccatac tcgaatgccg
    35401 tgaagtcgct ccacgaccga atggccagga gctcggccgc ccgccttgtc ggcatcgtgc
    35461 cttcgccgta atgctcgtgc tcggtcatac agaccttgtt gccgacccgg gcctgggcgt
    35521 ggatcgcgtc gaggccggtc acctgggccg atgcggggag cggagcgaaa agcagggcca
    35581 gtggaacgac agcaagccag cgccgggtca tcatcatgcc tccatatgcc tgtgaaatcg
    35641 aggccgatga tagcgccacc tccggttgcg gtgccggccg tttgcgtctt atggctaagc
    35701 ctgggttaag tatgcgctgc acccgtcgca gcgagcagat gaagcatcac acaggaacgc
    35761 aatgccctgc acattttcgc gcctgacatg ctggctggca gtatttgcgg ctctcctgct
    35821 cagcgcgcct gcgccggcca ctgcccagtc ctgcacgaaa gatgatatct ccaggacgat
    35881 cgactccacg gcggcggcgc tccgcaagtt gaacgcggcg caccaaccgg agctgcaagc
    35941 aaagctgcgg caactcggtg agctgcgggg ctggtcggag tccgagcaac cgaccaaggc
    36001 ggccgcgtat attcaggacg acgagacgcg agcactcgac gagcaggcgg gccggctgct
    36061 gctggagctc gaggttcttg gtgacgagag cgcgatcgac aaagagacct gtctcgcccg
    36121 attggagaag ctgaagaaga tttcggccca gctcatcgag atcacgacgg ccaaggcggc
    36181 tcacgtttca gccaaggtcg atgccgccct gaacgccggg acggcaccca agcgcgccgc
    36241 ggagcagcc
//
LOCUS       DASVDU010000089         5369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_230772, whole genome shotgun sequence.
ACCESSION   DASVDU010000089 DASVDU010000000
VERSION     DASVDU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230772"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VF226_05895"
     CDS             <1..113
                     /locus_tag="VF226_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7073556.1"
                     /translation="MSVELITPIAMEEKQRFAIREGGRTVGAGVVTKIVE"
     gene            265..340
                     /locus_tag="VF226_05900"
     tRNA            265..340
                     /locus_tag="VF226_05900"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:298..300,aa:Trp,seq:cca)
     gene            451..648
                     /gene="secE"
                     /locus_tag="VF226_05905"
     CDS             451..648
                     /gene="secE"
                     /locus_tag="VF226_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX7073557.1"
                     /translation="MAKYNPIEFIQEVRQETSKVTWPGRKEVWVTTVAVLVMVSLAAI
                     FFLVTDQVLGWLVSLVLGFGR"
     gene            714..1247
                     /gene="nusG"
                     /locus_tag="VF226_05910"
     CDS             714..1247
                     /gene="nusG"
                     /locus_tag="VF226_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX7073558.1"
                     /translation="MAKRWYIVHAYTNFERKVADAIRERARQAGLEHLIEDIVVPTEE
                     VIEVRRGRKVPTERKFLPGYVLVKMELTDQVFHLIRNTPKVTGFLGAENKPMPISEEE
                     AARILHQVREGVERPKPSVTFEIGEQVRVADGPFASFSGSVEEVDEERSRLKVAVSIF
                     GRPTPVELEFGQVEKVS"
     gene            1410..1838
                     /gene="rplK"
                     /locus_tag="VF226_05915"
     CDS             1410..1838
                     /gene="rplK"
                     /locus_tag="VF226_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7073559.1"
                     /translation="MAKKIEGYINLQVPAGKANPSPPIGPALGQRGVNIMEFCKAFNA
                     KTKDMEPETPIPVKITVYSDRSFTFEMRTPPATYFLKKAAKINAGSKEPGRSSVGTVT
                     MAQVREIAKEKMKDLNTDDLEQAAKTIAGSARSMGLQVVE"
     gene            1844..2545
                     /gene="rplA"
                     /locus_tag="VF226_05920"
     CDS             1844..2545
                     /gene="rplA"
                     /locus_tag="VF226_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7073560.1"
                     /translation="MAKVGKRIREARAKVDRNKAYPLDEAIKLVKATSSVKFDETVDI
                     ALNLGVDPKHADQVVRGVCQLPNGSGRTVRVAVFARGAKAEEAKEAGADVVGAEDLFE
                     QVQRGEINFDRCIATPDMMGLVGRLGKVLGPRGLMPNPRVGTVTMDVAGAVKAAKGGA
                     IEFRVEKAGIVHAGVGKASFTEEQLAENIRAFVDAVNRAKPSGAKGTYLKKVAISSTM
                     GPGVRVDQATITGAA"
     gene            2928..3446
                     /gene="rplJ"
                     /locus_tag="VF226_05925"
     CDS             2928..3446
                     /gene="rplJ"
                     /locus_tag="VF226_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7073561.1"
                     /translation="MDRAAKRELVASMHDVFKDTGVIVVAHYAGMTVAQMTELRRRVK
                     EVGGTVKVAKNRLAKLALRETEAEGIADLFQGPTCIAYSADPIAAAKAAVTYSRENDK
                     LVILGGAMGATVLDPAGVKALADLPSLDELRAKLIGLIQAPATKIARVLNEPGAQLAR
                     VLQAKATKEEAA"
     gene            3529..3906
                     /gene="rplL"
                     /locus_tag="VF226_05930"
     CDS             3529..3906
                     /gene="rplL"
                     /locus_tag="VF226_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7073562.1"
                     /translation="MTDLAKIVDDLSNLTVLEAAELAKMLEEKWGVSAAAAAVAVAPA
                     PGAAAAAPVEEQTEFTVILKSAGDKKINVIKEVRAITNLGLKEAKDLVEAGNKPVKEG
                     VPKDEAEKIKAQLEAAGATVEIK"
     gene            4105..>5369
                     /locus_tag="VF226_05935"
     CDS             4105..>5369
                     /locus_tag="VF226_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7073563.1"
                     /translation="MAETFNGRRRFRKQFGHIPEVAEMPNLIEVQKSSYDDFLMVKEP
                     PGGRPDDQGLQAVFKSVFPISDFSGRATLEFVRYDFEPPKYDVDECMQRDMTYSAPLK
                     VKLRLIVFDINEETGSRSIKDVKEQDVYMGDMPLMTANGTFVINGTERVIVSQMHRSP
                     GVFFDHDRGRTHASGKLLFAARIIPYRGSWLDFEFDAKDLVHVRIDRRRKLPVTTLLY
                     ALGLDDEEILSSFYNHILIKEAKRGWRMPFTPEKMRGTAPAADLVDAKTGEVVVRAGE
                     KITARRARELSQSGLKEVLVSADDLAGRYLAEDIVDMDTGQIYGEAGDEIDGKLLAVL
                     KERGIKQFPILDIDHVTTGAFIRNTLKVDKNANREEALMDIYRVMRPGEPPTVEAAET
                     MFHGLFFDPERYDLSAVGRVKMNMRLDLDA"
BASE COUNT         1050 a         1597 c         1836 g          886 t
ORIGIN      
        1 cgatgagcgt cgagctgatc acgccgatcg cgatggagga gaagcagcgg tttgcgatcc
       61 gcgagggcgg tcgcacggtc ggcgccggcg tggtgacgaa gatcgtggag tgaaaagcgg
      121 gccagaaaca ctggcggctg cggctgctgc ggctccgggg cacgctccgg accggtcggg
      181 tgacttgtca agaaaaaaat gtgacaaggc ttgcgtgatc ggttatcaag ggcgttcttg
      241 aggggccgaa ctctggcagc ttctaggggt gtagctcaat tggcagagcg tcggtctcca
      301 aaaccgaagg ctgggggttc gagaccctcc acccctgcca tttgaacaac atgttcgccc
      361 cggggctcgg cgttgccggc cacggggtgt cgtcatcagc ggggcgctca cggcggggcg
      421 cgggtcgtgc ggcccggagt atcagcggga atggcaaaat ataaccctat cgagttcatt
      481 caggaggttc gccaggagac ctccaaggtc acatggcctg ggcgcaagga ggtgtgggtc
      541 accactgtgg ccgttttggt catggtatcg ctggcggcca tcttcttcct tgtgacggac
      601 caggttctgg gctggcttgt cagtctcgtc cttgggtttg gccgctgatc gacggggcgt
      661 tggtctgacg ggcagtgcgc gaaagcagct aataatcgca ggatccgaga cgaatggcga
      721 agcgttggta catcgtgcac gcttatacca attttgagcg caaggtggcc gacgccatcc
      781 gcgagcgcgc gcggcaggcg gggctcgagc acctgatcga ggacatcgtc gttccgaccg
      841 aggaggtgat cgaggttcgg cgcggtcgca aggtgccgac ggagcgtaag ttcctgccgg
      901 gctacgtcct cgtcaagatg gagctgaccg accaggtgtt ccacctgatc cgcaacacac
      961 ccaaggtgac gggttttctc ggagccgaga acaagccgat gccgatctcc gaggaggagg
     1021 ccgcgcgcat cctgcatcag gtgagggaag gcgtggagcg cccgaagccg tcggtcacgt
     1081 tcgagatcgg cgagcaggtg cgggtggccg atggaccatt cgcctcgttc tcgggctcgg
     1141 tcgaggaggt ggacgaggag cgctcgcggc tcaaggtagc tgtttcgatc ttcggccggc
     1201 cgacaccggt tgagctcgag ttcgggcaag tggaaaaggt cagctgagcg catgcgcagc
     1261 cgaccacggg atccggcccg actgggccgg gagtgccgcg gcatcgaccc atccggctcg
     1321 tgccgcatcg tgcgcgggag ggcttgccag cccgttaacc gctaacccag gagggggtca
     1381 ccccttcact gtggctaaag gatgactata tggcaaagaa gatagagggt tacatcaacc
     1441 tgcaggttcc ggcgggcaaa gccaacccat cgccgccgat cggccccgcg ctcggacagc
     1501 gcggcgtcaa catcatggaa ttctgcaagg cgttcaacgc caaaaccaag gacatggagc
     1561 cggagacgcc catcccggtg aagatcaccg tttactccga ccggtccttc acgttcgaga
     1621 tgcgcacgcc gccggcaaca tacttcctca agaaggcggc gaaaattaac gctgggtcca
     1681 aggagcccgg ccgctccagc gtcggcaccg tcacaatggc gcaggtgcgg gaaatcgcca
     1741 aggagaagat gaaggatctc aacaccgacg atctcgagca ggccgcgaag acaatcgctg
     1801 gttccgcgcg gtcaatgggc ctgcaggtgg tggagtgacg gccatggcga aagtcggaaa
     1861 gagaatcaga gaggctcgtg cgaaggtcga tcgcaacaag gcctatccgc tcgatgaagc
     1921 gatcaagctg gtgaaggcga cctcatcggt gaagttcgac gagaccgttg atatcgcgct
     1981 caatctcggc gtcgatccga agcacgcgga ccaggtcgtg cggggcgttt gccagctgcc
     2041 gaacggctcg gggcggacgg tgcgcgttgc ggttttcgca cgcggcgcca aggccgaaga
     2101 ggccaaggag gccggcgccg atgtggtagg cgccgaggat ctcttcgagc aggtccagcg
     2161 cggcgagatc aacttcgacc gctgcatcgc gacgccggac atgatgggcc tggttggccg
     2221 cctcggcaag gtgctgggtc cgcgcggcct gatgccgaac ccgcgcgtcg ggacggtgac
     2281 tatggacgtg gcaggtgccg tgaaggccgc caagggcggc gccattgagt ttcgcgtcga
     2341 gaaggcgggc atcgtgcacg caggcgtcgg caaggcgagc ttcaccgagg agcagcttgc
     2401 cgagaacatc cgcgcgttcg tcgatgccgt gaacagggcg aagccgtcgg gtgcaaaggg
     2461 gacatacctg aaaaaggtgg ctatcagctc gacgatgggc cccggggtcc gcgtcgatca
     2521 ggcgacgatc accggcgctg cctagtcgag gtttcggaaa gggcggcatg ggtgggggcg
     2581 tgaaatccct gccttgcgtg agtgagacga cgagcttccg ggcgctccaa gcccggtgga
     2641 ccgagccgat gccaccttgt gtgggattgg ctcaaacctg tccgagactg caggcgccgg
     2701 cgctgccggc ttaatcactt gggcctgcat gagacgggag acgatccgga actcggggcc
     2761 ttcgggcctt gcattccggt ttgagcctct tgggtcgcac cgtgccgttg tgcgcggttg
     2821 gacaggtcga gcgccgcagg cgtgggcaat catccctgaa gccggcggca gggtgcaacc
     2881 cgcggacgat cctcgcaaga ggatagtccg caagatggag agagcaagtg gacagagctg
     2941 caaaacgcga gctcgtggcc tcgatgcacg acgtgttcaa ggacactggt gtcatcgtcg
     3001 tcgcccacta tgccggtatg acggtcgccc aaatgaccga gctgcgccgc cgcgtgaagg
     3061 aggtgggtgg cacggtcaag gtggccaaga accggctggc gaagcttgca ctccgggaaa
     3121 cggaagccga gggtatcgcg gacctgttcc agggtccgac gtgcatcgcc tattcggccg
     3181 atccgattgc cgcggcaaag gccgccgtca cgtactcgag agagaacgac aagctcgtga
     3241 ttctcggggg cgcgatgggt gccaccgtgc tggatcccgc aggagtgaaa gcgctcgccg
     3301 atctgccctc gcttgacgag ctcagggcga agctcatcgg cctcatccag gcgccggcga
     3361 cgaagatcgc ccgcgtgctc aacgagccgg gtgctcagct cgctcgcgtc cttcaggcga
     3421 aagccacgaa ggaagaggcg gcatgaggca agggcgctga cgaagccacc gggcccgcaa
     3481 ggcccacact atcactgaaa caggttcaaa ccggaaggaa ctgcaaccat gaccgatctc
     3541 gcaaagatcg tcgatgatct ctcgaacctc accgttctgg aagctgccga gctcgccaag
     3601 atgctcgagg agaaatgggg cgtttcggcc gctgcggccg ccgtcgccgt tgctcctgcg
     3661 cctggtgctg cggccgccgc tcctgtcgag gagcagaccg agttcactgt catcctgaag
     3721 tcggccggtg acaagaagat caacgtcatc aaggaggtcc gtgcgatcac gaacctcggc
     3781 ctgaaggagg ccaaggacct cgtcgaggcc ggcaacaagc ccgtcaagga gggcgtgccg
     3841 aaggacgagg ccgagaagat caaggctcag ctcgaggccg caggcgcgac cgtcgagatc
     3901 aagtgaggct gcgctccctc cccctgcccg agcgggggga gggccattgc gggacggttc
     3961 cccggaggtt tcggcagggt tcatcagccc tcgtccgtcg cctccggcga gccgtttcag
     4021 aaggcgcggg gggtggcggc ccctccgaca gaagcacgaa gggcgcggga gtcgtcaccc
     4081 gcgacgagaa tctaaggagc ggatatggct gagacgttca acggccgcag gcggttccgg
     4141 aagcagttcg ggcacattcc cgaagtggcc gagatgccga acctcatcga ggttcagaaa
     4201 agttcctacg atgacttttt gatggtgaag gagccgccgg gtggccggcc ggacgaccag
     4261 ggcctgcagg ccgtgttcaa gtccgtgttc cccatttccg atttttccgg ccgggcgacc
     4321 ttggaattcg tccgctacga cttcgaaccg ccaaagtacg acgtcgacga gtgcatgcag
     4381 cgggacatga cctattcggc cccgctgaag gtcaagctgc gcctgatcgt cttcgacatc
     4441 aacgaggaaa ccggctcgcg ttcgatcaag gacgtcaagg agcaggacgt ctacatgggc
     4501 gacatgccgc tgatgaccgc gaacggcacg ttcgtcatca acggcaccga gcgcgtcatc
     4561 gtctcccaga tgcaccgttc cccgggcgtg ttcttcgacc acgaccgcgg ccggacccac
     4621 gcctcgggca agctgctgtt cgccgcgcgc atcatcccgt atcgcggctc ctggctcgac
     4681 ttcgagttcg atgccaagga tctcgtgcac gtgcgcatcg acaggcgccg gaagctgccg
     4741 gtcacgacgc tgctgtacgc cctgggcctc gatgacgagg agatcctttc cagcttctac
     4801 aaccacatcc tcatcaagga agccaagcgc ggctggcgga tgccgttcac gcccgagaag
     4861 atgcgcggca ccgctccggc tgcggatctc gtcgacgcca agacaggcga ggtggtcgtg
     4921 cgcgcgggcg agaagatcac ggcgcgcagg gctcgcgagc tttcgcagag cggcctcaag
     4981 gaggtgctgg tttccgcgga cgacctggcg ggccgctatc tcgccgagga tatcgtcgac
     5041 atggacaccg gccagatcta tggcgaggct ggtgacgaga tcgacggcaa gctgctggcc
     5101 gtgctcaagg agcgcgggat caagcagttc ccgattctcg acatcgacca tgtcacgacg
     5161 ggcgccttca tccggaacac gctcaaggtc gacaagaacg ccaaccgcga agaagcgctg
     5221 atggacatct accgggtcat gcggcccggc gagccgccga cggtcgaagc ggccgaaacg
     5281 atgttccacg gcctgttctt cgatccggag cgctacgatc tgtcggccgt cggccgcgtg
     5341 aagatgaaca tgcgcctcga cctcgacgc
//
LOCUS       DASVDU010000090         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1585861, whole genome shotgun sequence.
ACCESSION   DASVDU010000090 DASVDU010000000
VERSION     DASVDU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585861"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VF226_05940"
     CDS             complement(<1..315)
                     /locus_tag="VF226_05940"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX7073564.1"
                     /translation="MVEVNRPAEIDEPVEVIEFRPEWAALFAFEAEALRRVLAVSAIE
                     HIGSTAVPGLAAKPVIDIQVGVAGPPDAQRVGGPLAALGYEGLGEAGVPGRLYFRRRG
                     AVA"
     gene            complement(944..2764)
                     /gene="mobH"
                     /locus_tag="VF226_05945"
     CDS             complement(944..2764)
                     /gene="mobH"
                     /locus_tag="VF226_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobH family relaxase"
                     /protein_id="HEX7073565.1"
                     /translation="MFALLRRKRVSLLSTPTAETHTDLLRPEPAAALLGTLRRQRLLE
                     QIWQRTSVSRAQFDLLYRLPIERYAALVQQFPASENHHHAYPGGMLDHGLESVAYALR
                     LRQSHLLPAGAPPETQAAQAEAWTAGAAYGALLHDLGKIAVDLHVEYANGTRWHPWHG
                     PLRHPYRFRYVSDREYRLHSAATGLLYTDILDSAILDWLSDFRELWVALLHLLAGRYE
                     HAGVLGELVLQADRASTAAALGGDPQQVRTSPTHALHRQLLDGLRFLVREQLKLNRSE
                     ASDGWLTADGLWLVSKTVCDKLRAYLLSQGIDGIPSTNTAVFDVLQEHGIVQPTPDGK
                     AIWRATVTSGNGWTHSFTFLRIAPTLIWDMNERPASFVGSVQVEDAVATSLSEAPPTA
                     SASPKSSSVAVASATDMSIAPEVTASDSVDHKVASPNVHTEGIHAPTDRAETDTKPTH
                     MLQPVPPSVETRSQHALSSAADESVGLQFLSWLRAGIQAHRLIINDAKALVHTVAGTA
                     FLVSPGVFQRYTQEHPEIARLAKRAGVAEWTWVQKQFEKLRLHRKQDNGLNIWMCEVA
                     GPRKTRRLHGYLLLDGHTLFTDMPCDNPYLKLLTERRNAA"
BASE COUNT          548 a          917 c          882 g          544 t
ORIGIN      
        1 agccaccgcc ccgcgccggc ggaagtacag tcggcccggc acgccggctt cgcccaaccc
       61 ctcgtacccc aaagcggcca gcggcccgcc gacccgctgc gcgtcgggag gcccggcaac
      121 tccaacctga atgtcgatga ctggcttggc cgccagcccc ggcaccgccg tgctgccgat
      181 gtgctcgatg gcggacaccg cgagcacgcg ccggagtgcc tcggcctcga aggcaaacag
      241 agccgcccac tccgggcgaa actcgattac ctccaccggc tcatcaatct cggccggccg
      301 attgacttcc accattgaag cgctcccgcc gaactttgga gttcatctac aacgattcat
      361 gctcgatcgc tgttcgctaa agatgccccg agcctcggcg cggtgacaac ggagaaaacg
      421 gtacatcgcc ttccgctttt ctatccacgc ttagccgatg tgctgtcctc tgtgggtcgt
      481 cagcgactgc tcgacgccgc atcgccagca ctctgacgat gctcctatca actgcgcgac
      541 agagcatcgc ttcagccaag cgtctgcgcc gcacgccacc agggaatagg cccagccacg
      601 agctgtcaga gagtcgatga gctgacgaag accgtcgccg gcgagccgga tgagccggaa
      661 atgcgccgca ctgaaacgag cggcatcctt gctctaacga gcacccaccg caacagctgc
      721 tcccctcgtg cgcgcacttg acgactgtgt ccgcgcatcg cccgaagagc tgcaagccta
      781 tctacaatag tccacctgga tcaaacgcat ctctccgaag cgcaggagat cgagctgttg
      841 cagtcgttat ggtcgacgat gtccaagttc gtcgaccgtg caattggctt atcaagaatc
      901 ctgtgcagca ggcacaggct tcgcgaagag ctagccggtg gctctaggcc gcattccgtc
      961 gttccgtcaa aagtttgaga taggggttgt cgcagggcat gtctgtgaac agcgtgtgcc
     1021 cgtcgagcag caggtagccg tgcaggcgcc gtgtctttcg aggtccggcc acctcgcaca
     1081 tccaaatgtt caagccgttg tcttgcttcc gatgaaggcg cagcttctca aattgctttt
     1141 gaacccaggt ccattcggcg actccggctc gcttggcgag gcgagcgatc tctggatgtt
     1201 cttgtgtgta acgctggaat actcctgggc tgaccaagaa tgccgtgccg gcaacggtgt
     1261 gaacgagcgc cttcgcatcg ttgatgatga gacgatgggc ttgaatgccc gcacgcagcc
     1321 acgacaggaa ttgaagtccg actgactcat cggctgcgct ggatagagca tgctgagatc
     1381 gtgtctcgac cgacggcggc acaggctgaa gcatgtgagt cggcttcgtg tccgtctcag
     1441 cgcgatctgt cggcgcgtgg atgccttcag tgtgcacatt gggcgacgcc accttgtgat
     1501 cgacagaatc gcttgccgtt acctctggtg cgatcgacat gtcagtcgcc gacgccactg
     1561 ccaccgacga gctcttgggc gatgcgcttg cggtcggagg cgcttccgac aaggaagtgg
     1621 ccacggcatc ttctacctga acggagccga cgaacgacgc agggcgctca ttcatgtccc
     1681 agatgagcgt gggagcgatg cgcaggaatg tgaagctatg tgtccagccg ttgccgctcg
     1741 tgacggtcgc tcgccagatc gctttgccgt caggcgttgg ctgcacgatg ccgtgctcct
     1801 gcagtacatc gaagaccgcg gtattggtgc tcgggattcc gtcgatccct tgtgacagca
     1861 ggtacgcgcg cagtttgtcg cagacggttt tcgatacgag ccacaggcca tccgccgtga
     1921 gccagccatc ggaggcttca ctccgattca gcttgagttg ttcgcggacc aggaagcgca
     1981 gtccatcgag aagctggcga tggagcgcgt gcgtgggcga cgtacgcacc tgctgcggat
     2041 caccgccgag agcggctgcg gtggaagcgc gatccgcctg caggaccaac tcgccgagca
     2101 cgccggcatg ctcatatcgt cccgcgagaa gatgcagcag cgctacccaa agctcgcgaa
     2161 agtcgctgag ccagtcgagg attgcggagt ccagaatgtc ggtgtacagc agccccgtgg
     2221 ccgcgctgtg aaggcgatac tcgcggtccg aaacgtaccg gaatcgataa ggatgtcgca
     2281 gcggtccgtg ccagggatgc cagcgcgtgc cgttggcgta ttccacatgc aggtcgacgg
     2341 cgatttttcc gagatcgtgc agaagggcgc cgtaagccgc gccagccgtc caggcctcgg
     2401 cctgcgccgc ctgcgtctcg ggcggcgcac cggcaggcag caggtgagat tgccgcagcc
     2461 gcagcgcata tgcgacggac tcgagaccgt gatccagcat tccacccgga taagcatggt
     2521 gatggttctc cgaggcgggg aattgctgga ccagcgccgc gtagcgctcg atcggcaagc
     2581 gatacagcag gtcgaattgc gcccgggaaa cggacgtgcg ttgccagatc tgttcgagca
     2641 gtcgctgacg gcgcaaagtg ccgagtaggg ccgcagccgg ctcgggtcgc agcaggtccg
     2701 tgtgcgtttc ggcggtcggc gtcgaaagca gagaaaccct ttttctacgc aacagcgcga
     2761 acatcgccat cacaaacgag ttgcccgcag ctcgaaccct tttcgccctt tacccagcct
     2821 tttcggatag ggcctttacc cttgctgccc cgtcccttcg cctttcgcca ttagccccaa
     2881 gccctttgcg c
//
LOCUS       DASVDU010000091         9459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_230452, whole genome shotgun sequence.
ACCESSION   DASVDU010000091 DASVDU010000000
VERSION     DASVDU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9459
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230452"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF226_05950"
     CDS             complement(<1..494)
                     /locus_tag="VF226_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HEX7073566.1"
                     /translation="MFIQIETTPDPAVLKFLPGRDVLGEGTLALHDSTEATNSPLAQR
                     LFEIPDVTGVVLGSNFITVTKNGGNWQHLKPAILGAIMDHFASGAPVLVQSNGAGAPE
                     TGEDGLVGRLREALRQVIDPELGYNIVDLGLVYGIAVEEGGVARVTMTTTTVGCPATN
                     YLVEG"
     gene            complement(564..1100)
                     /locus_tag="VF226_05955"
     CDS             complement(564..1100)
                     /locus_tag="VF226_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX7073567.1"
                     /translation="MATQFVDLDVRPTLRAGGEPFEEIMQAVAGLRPGEGLRLYATFK
                     PVPLFSVLGARGFVHEERELEGGDWEVLFRPNGVAEPVSAAAKPAASTDDEEWPEPVQ
                     HLDNRELDPPEPMVRILATTETMKEGEVLSALLCREPIFLFPELQKRGHVWRGGFEPD
                     GKTYRILVRVGANKNGAA"
     gene            complement(1119..2471)
                     /locus_tag="VF226_05960"
     CDS             complement(1119..2471)
                     /locus_tag="VF226_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073568.1"
                     /translation="MIGVSLSRWTMSYFAAALLALVAAEALMVAGYGFPNDALRAPQT
                     LVLVHLVAIGWLSLLMCGALFQFVPVLIARPLYSNTLTLPTLICLLAGLAMLLLGFVQ
                     LTGHIEPGLPFLALAAALLGVGFALVLWNLGRTLWQARPPLPAQLVVVGLVSLAATAV
                     YGIIFALVLGGFVDHEPCISIAASGLPLHAIAGLGGWLTFTAVGVSYRLLAMFMLAPE
                     LDNWRNRAVLYTGSGALAIVILGGAAAIRLGFEIDFVLGLAGVLGLVTVALYGADVVH
                     LYRARKRRNIELNSRMAAFALASLAASAVLIAVLTLLGRLADHVGAVVFLIAFGWLSG
                     LGLSKLYKIVAFLTWLECYGPVLGKAATPRVQDLVVEKRAIKWFVIYFIAVWVGTVAL
                     LVGHASHFQIAAAGMLIGTLGIIVQLVRSRQLADVDAERRIPEGAYRPRLLLSLARPD
                     "
     gene            complement(2536..2778)
                     /locus_tag="VF226_05965"
     CDS             complement(2536..2778)
                     /locus_tag="VF226_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEX7073569.1"
                     /translation="MTSPNVVDVRSFVPAERHRRIFELVDKLEPSSSFILVNDHDPKP
                     LYYQLEAEYPKQFSWTYLERGPQVWRVEIGKVAQSS"
     gene            complement(3104..3259)
                     /gene="ccoS"
                     /locus_tag="VF226_05970"
     CDS             complement(3104..3259)
                     /gene="ccoS"
                     /locus_tag="VF226_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX7073570.1"
                     /translation="MEALVYLIPISIGLGGIGLAAFMWSLKTGQYEDLAGAAERILFD
                     DNDRPQG"
     gene            complement(3268..4098)
                     /locus_tag="VF226_05975"
     CDS             complement(3268..4098)
                     /locus_tag="VF226_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX7073571.1"
                     /translation="MDRLPPVVPPLPGTKTDWRCNLPPATAFDWLAAGWKDFTIRPAL
                     SVAYGLFVFAISIAVVGGLFLFGYDYILFPALSGFLVVGPLIAAGLYEKSRRIEKGAP
                     APATIAGMLIVKPKSNGQMLFMGMLLCLLMVLWMRAAVLLYALFFGLLPFPGLHDIVA
                     ILIGTPKGWALIVVGSLVGGLFAAFSFAISVFAVPMLLDQRTDALTAMGTSMALVWNN
                     LPVMLSWGAIVVALFALSVASGLVGLIVAFPVLGHATWHAYGAIRAKVIAAGGLDEAQ
                     "
     gene            complement(4135..4317)
                     /locus_tag="VF226_05980"
     CDS             complement(4135..4317)
                     /locus_tag="VF226_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7073572.1"
                     /translation="MRTRTRRRLLVGLAIATGLGIIGAANAHLVWASLKSQPECVPHV
                     KAPGHNGQFRAAKSSC"
     gene            complement(4314..5195)
                     /gene="ccoP"
                     /locus_tag="VF226_05985"
     CDS             complement(4314..5195)
                     /gene="ccoP"
                     /locus_tag="VF226_05985"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX7073573.1"
                     /translation="MGVKERDPHTGHMTTGHEWNGIIELNTAVPKPAWYFLIATALFS
                     LVYWVLMPAWPLGKTYTKGLLGADVRKNVETSLQKAALDRARWTDRIQKESFAAIEAD
                     EDLMKYVRETGRTLFGDNCAVCHGTKATGGPGFPSLVDNAWLWGGTPEAVFETIRVGI
                     NSDHPDSRIAEMLAFGRDGMLDRQSILDVVAYVRSLSDQSNASGKDAETIATGQEIFA
                     NNCSSCHGDDAKGNNDMGAPNLTDDFWIYGGDEESVYRTVYGGRQGRMPAWENRLSET
                     DRKILTLYVLDLGRSKP"
     gene            complement(5186..5359)
                     /locus_tag="VF226_05990"
     CDS             complement(5186..5359)
                     /locus_tag="VF226_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX7073574.1"
                     /translation="MIGLDHATLVAFAKSWGLFYLIAFSVAILAYTFWPGSGKRFDRA
                     ASSIMDDENGPWA"
     gene            complement(5356..6093)
                     /gene="ccoO"
                     /locus_tag="VF226_05995"
     CDS             complement(5356..6093)
                     /gene="ccoO"
                     /locus_tag="VF226_05995"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEX7073575.1"
                     /translation="MFGSHYKLEKNSIALAIGIIIVASIGGFVEIAPLFTIDETVEEA
                     PDMRVYTPLELAGRNIYMREGCYACHSQMIRTLRDEVERYGPYSLAVESKYDHPMLWG
                     SKRTGPDLARVGGKYSDQWHVAHLIDPRDVVPQSIMPSYRYLMRAKLKTDDLPQHLAA
                     LKKLGVPYTDEMIENAAQDAYGQSAPDSEQAGGAKARYGEATNIRSFDGDPGRLTEMD
                     ALVAYLQILGRLTDEAYKQKQMAEAGQ"
     gene            complement(6099..7757)
                     /gene="ccoN"
                     /locus_tag="VF226_06000"
     CDS             complement(6099..7757)
                     /gene="ccoN"
                     /locus_tag="VF226_06000"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX7073576.1"
                     /translation="MVSQLTRSERQLALIISIAVALWGLIMATAGRDDLLGGHGFIVL
                     ICGVAAAFFVLSHYDAPEPGEERFTQYYDDPIKAGIVLAMVWAVFAMFIGDWVSWLLA
                     YPNLTFDAGWASFGRLRPVHTTGVIFGFGGNALIATSFHVMQRTSRARMPDQLSPWFV
                     LIGYNLFCVLAVSGYLMGITQSKEYAEPEWYADIWLVIVWVTYLVLYLRTLARRQEPH
                     IYVANWYYMAFILVVAALHIVNNLAIPISFSSAKSYSAFSGVQDAMTQWWYGHNAVAF
                     FLTAGFLGMMYYYLPKRANRPIFSYRMSILGFWGITFFYMWAGSHHLHYTALPQWVQT
                     LGMTFSVMLLVPSWLAAANALLTLNGAWDKVRDDATLRFMMVAAAFYGLSTFEGSFMA
                     IRAVNSLSHYTDWTIAHVHAGTLGWVSMITFGAVYSLVPQLWKRPRMYSPALVEVHFW
                     LAIAGTLVYVLAMWNSGIIQGLMWRTYNESGTLAYSFIDSLVAMHPYYIARAIGGLLF
                     LSGAAVGFYNVCMTIREATSPAHEMAKDKPVAALAPARAIEAGE"
     gene            complement(8083..8979)
                     /locus_tag="VF226_06005"
     CDS             complement(8083..8979)
                     /locus_tag="VF226_06005"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7073577.1"
                     /translation="MKEPVMVEPLPKVDLTRLTLRDLVGIANAIEQDAMRRYGRLADE
                     MERRGERATAQAFRRMQAEEHKHAAAVEQWASELGEEVPPAEDFQWRLPAELSAAWDE
                     IAGSALVTPYRAFAIGVRSKERAFSMYSYLAAHARDPAVAAAAEKLAVEALQHAVLLR
                     RWRRSAYHLGLPRSSRLTIGTVEELRTFLAEREAAIADHHRALAGKLRKLGDEEGARL
                     LEELSGPPAPAHTAALVDDGASKSRLSLLVTAQKPLEALSEELETILASATGPLSAEA
                     EKALSNVIVRLARIASLVDRRT"
     gene            9124..>9459
                     /locus_tag="VF226_06010"
     CDS             9124..>9459
                     /locus_tag="VF226_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7073578.1"
                     /translation="MGRIVIIFLAWCLLGVPAWSAEGGGSELQAYLAQLSASDLVPGA
                     DRFGPVEGQPPIAPVYRGRELIGHAFLNSDHANAVGYSGKPIHIVVGIDTEGTITGLK
                     LVHHSEPIVL"
BASE COUNT         1910 a         3048 c         2885 g         1616 t
ORIGIN      
        1 ccttccacca gatagttcgt cgccgggcaa cccactgtgg tggtggtcat ggtcacacgc
       61 gcaacgccgc cttcctcgac ggcaatgccg tagacgagac caaggtcgac gatattgtag
      121 ccgagctcag gatcgatcac ctggcgaagc gcttccctca gtcgaccgac gagcccatcc
      181 tcaccagtct ccggcgcacc cgcgccgttg gattggacga gcaccggggc gcctgatgcg
      241 aagtggtcca tgatcgcgcc gagaatcgcg ggcttgagat gctgccagtt tccgccgttc
      301 ttcgtgaccg tgatgaaatt gctgccgaga acgactcccg tcacgtccgg aatttcgaac
      361 aggcgctgtg cgagtggcga attcgtagcc tccgtgctgt cgtgcagcgc gagcgtgccc
      421 tcgcccagca catcccgccc gggcaggaat ttcaggactg cagggtccgg agtggtttcg
      481 atctgaatga acatggctca caagctccgt aagggctggc ggccggcgca gccaagccgc
      541 atcttccgat gcggccgctg cattcatgcc gccccattct tgtttgcgcc gacccgcacg
      601 aggatccggt aggtcttccc gtctggctcg aagcccccac gccagacatg gccgcgcttc
      661 tgcagctccg ggaacaggaa gatcggctcg cggcacagca gtgccgaaag cacctcgccc
      721 tccttcatgg tctcggtagt cgccagaatg cgcaccatcg gctcgggtgg atcaagctca
      781 cggttgtcga ggtgctgaac cggctccggc cactcctcat cgtccgtgga cgcggcgggc
      841 tttgcggctg ccgaaacagg ctcggcaacc ccgttcggcc ggaacagcac ctcccagtca
      901 ccgccctcga gctcccgctc ttcgtgcacg aaacctctcg cgcccagcac gctgaaaagc
      961 ggcaccggct tgaacgtcgc gtaaaggcgc aggccctccc ctggccgcaa tccagcgacg
     1021 gcctgcatga tctcttcgaa cggctctccg ccggcccgca gggtcggacg gacgtccaga
     1081 tccacgaatt gggttgccat catcagtctc cttttcgatc agtcggggcg agcaagagaa
     1141 agcaagagcc gcggcctgta tgccccctcg gggatgcggc gctctgcatc cacatccgcc
     1201 agctgacggg agcgcacgag ctgcacgatg atgcccagcg tcccgatcag catccctgcg
     1261 gccgcgatct ggaaatgcga ggcgtgcccg acgagcagcg cgaccgtgcc gacccatact
     1321 gcgataaagt agatgacgaa ccacttgatc gcgcgcttct cgaccacgag atcctgcacc
     1381 ctgggcgtcg ccgcctttcc gagcaccggg ccgtagcact cgagccaagt gaggaaggcg
     1441 acgatcttgt agagcttgga caggccgagg ccggaaagcc atccgaaggc gatcaaaaag
     1501 acgacggcgc cgacatgatc cgcaagccgc ccgagtaaag tgagcacggc gatgagaacg
     1561 gccgatgccg ccagcgaagc aagggcgaag gccgccatgc ggctgttgag ctcgatattg
     1621 cgccgcttgc gtgcgcgata gaggtggacg acatcggccc cataaagcgc gaccgtaacg
     1681 agcccgagca cgccggcaag ccccaagacg aagtcgatct cgaagcccag acggatcgcc
     1741 gctgcgccgc ccagaatgac gatcgccaat gcacccgaac cggtgtagag gaccgcgcgg
     1801 tttcgccagt tatccagctc cggcgccagc atgaacatgg cgagaagccg atagctgacg
     1861 cctacggcgg tgaaggtcag ccatccgccc agtcctgcga tggcatggag tggaaggccc
     1921 gatgccgcga tggaaatgca tggctcgtga tcgacgaacc cgccgaggac gagcgcaaaa
     1981 atgattccgt agaccgcggt cgctgcgagg ctcacgagtc cgactacgac caactgggcc
     2041 ggtagtggag gccgcgcctg ccaaagcgtc cggccgagat tccagagcac gagggcaaag
     2101 cccacaccca acagcgctgc ggcgagagcg aggaaaggca gccctggctc gatgtgaccg
     2161 gtaagttgca caaagcccag cagaagcatc gccaagccgg ccaacaggca gatcaacgtc
     2221 ggcagcgtca gcgtattgct gtagagcggc cgcgcgatga gcacgggcac gaactggaac
     2281 aaggctccgc acatcagcag gctgagccag ccgatcgcca ccagatgaac gagaacgagg
     2341 gtctgaggcg ctctcaacgc atcgttgggg aacccgtagc cggccaccat gagcgcctcc
     2401 gctgcaacaa gcgctaagag ggcggccgca aagtaggaca ttgtccagcg ggagagggag
     2461 acaccaatca tggcagctcc agggccagcg cgtgccggac ttggccagcc cggcaacgcg
     2521 cccgcaaaaa acacctcagg aagactgtgc gaccttaccg atctcgacgc gccacacctg
     2581 tggtccgcgc tcgagatacg tccaggagaa ctgcttcgga tactcagctt cgagctggta
     2641 atagagcggt ttcggatcgt gatcgtttac tagaatgaag gacgagctcg gctcgagctt
     2701 gtcgacgagt tcaaaaattc ttcggtgccg ctccgccggg acgaagctgc gaacatcgac
     2761 gacattggga ctagtcatgg acaggctcct ttggtcgcat gtctgggtac ggcggttcag
     2821 cggctgcgac cggaatcgcc ggctctatga cgcggacgtt cgccgcaatc ggcttccctg
     2881 catcacaggc tgaccgtgac cagagtgaac gccattaaag atacattgtc aatatatctt
     2941 ttggagcgag gtgagatgtc gacgtcacga aagggctggc aaacgccact tgcctcagcc
     3001 caacggatga gctgagcacc gcatgtctcg aataagagaa aaaattggaa caggaacgcc
     3061 gccgcaaagg agcccgcaga tttgatctgc tgtttcgaat ggctcaacct tgcgggcgat
     3121 cgttgtcgtc gaaaaggatg cgttcggcag ccccggccag gtcttcgtac tggccggtct
     3181 tgagggacca catgaacgcc gccaatccga taccccctag gccaatggat atcggaatga
     3241 ggtaaacgag agcctccatg gctcacctca ctgagcttcg tcgagaccgc ccgcagcgat
     3301 cacctttgcc cgaatcgcgc cgtaggcgtg ccacgtcgca tggccgagca ccgggaaggc
     3361 aacgatcaat ccgacgaggc cgctcgccac gctgagagcg aacagcgcaa ccacgatcgc
     3421 gccccaactc agcatcacgg gcaagttgtt ccagacgagt gccatgctcg ttcccatcgc
     3481 ggtcagcgca tcggtgcgtt ggtcgagaag catgggcacg gcaaaaacgc tgatcgcgaa
     3541 agaaaaggca gcaaacaatc cgccgacaag gctgccgacg acaatgaggg cccagccctt
     3601 cggcgttccg atcaatatcg ccacgatgtc gtgaaggccc gggaacggca ggaggccgaa
     3661 gaagagcgcg tagaggagaa ccgcggcgcg catccagagc accatcagca gacacaggag
     3721 catgcccatg aacagcatct ggccgttgga tttcggcttc acgatcagca taccggcgat
     3781 cgtcgcagga gccggagcgc ccttctcgat gcgtcggctc ttctcataaa gaccggctgc
     3841 gatcagcggt ccaaccacca ggaatcccga caacgctgga aagaggatat agtcgtagcc
     3901 gaacaagaaa agcccgccga caacagctat cgagatggca aaaacaaaca ggccataagc
     3961 gacgctcaaa gccggccgga tggtgaaatc cttccaccct gccgcgagcc aatcgaacgc
     4021 agtcgcgggc ggtagattgc agcgccaatc ggttttcgtg ccgggaagcg gcggaacgac
     4081 cggggggagc ctgtccatat tcttcgccac tgccattgcc cttaccctct cgcttcagca
     4141 ggacgatttt gccgcgcgga actgcccatt gtgccccggc gctttcacat gcgggacgca
     4201 ttcgggctgg gacttcaacg acgcccacac gagatgtgca ttggctgccc cgatgatccc
     4261 gagccccgtc gcgatggcga ggccgacaag gagacgtctt ctcgtccgcg ttctcatggt
     4321 ttactccgcc cgaggtcgag aacgtaaagc gtgagaatct tgcggtcggt ctcgctgagg
     4381 cgattttccc aagcaggcat ccgcccctgc ctgccgccgt aaacggtgcg ataaacagat
     4441 tcttcgtcgc cgccataaat ccagaagtcg tccgtcaggt tcggcgcgcc catatcgttg
     4501 ttgcccttgg catcgtcgcc gtgacacgag ctgcaattat tcgcaaagat ctcctggccc
     4561 gtggcgattg tctcggcgtc cttgcctgat gcattcgact gatcggaaag cgagcgcaca
     4621 taggctacga catcgaggat cgactggcgg tccagcatcc cgtcgcgtcc gaaggccagc
     4681 atctcagcga tccgactgtc gggatgatcg gaattgatgc cgacccggat cgtctcgaac
     4741 accgcctccg gggttccgcc ccacagccac gcgttgtcga cgagactggg gaagcccggc
     4801 ccgccggtcg ccttggtccc gtgacagacg gcgcaattgt cgccgaagag cgtgcgcccc
     4861 gtctcgcgca cgtacttcat cagatcctcg tcagcctcga tggcggcaaa gctctccttt
     4921 tggatacgat ccgtccacct cgcccggtcg agcgccgcct tctgcaggct tgtctcgacg
     4981 ttcttgcgca catcggcacc gaggaggcct ttcgtatagg tcttgcccag cggccaggct
     5041 ggcatcagca cccagtagac gagcgagaac agcgccgtcg cgatcaggaa gtaccacgcc
     5101 ggcttcggta ccgccgtatt cagctcgatg ataccgttcc actcatggcc ggtcgtcatg
     5161 tggccggtat ggggatcgcg ttccttcacg cccatggccc gttctcgtca tccatgatgc
     5221 tgcttgccgc ccggtcgaac cgcttcccgc ttcccggcca gaacgtgtag gcgaggatgg
     5281 ccaccgaaaa cgcgatcagg tagaaaagcc cccacgactt cgcaaaggcc accagcgtcg
     5341 cgtggtcgag cccgatcatt gccccgcctc cgccatctgc ttctgcttgt aggcttcatc
     5401 ggtcagccgt ccgagaatct gcagataggc aacgagtgca tccatctcgg tgagcctgcc
     5461 gggatcgccg tcgaacgagc ggatattggt ggcctcgccg tagcgcgcct ttgccccccc
     5521 ggcctgctcg ctgtcgggcg ccgactggcc gtaggcgtcc tgcgcggcgt tctcgatcat
     5581 ctcgtccgtg tagggcacgc cgagcttctt cagtgccgca agatgctgcg gcaggtcgtc
     5641 ggtcttgagc ttcgccctca tgaggtagcg gtagctcggc atgatcgact gaggcacgac
     5701 gtcccgcggg tcgatcagat gcgcgacgtg ccattggtcc gagtacttgc cgccaacgcg
     5761 cgccagatcg gggccggtgc gcttcgaccc ccacagcatc ggatggtcgt atttcgactc
     5821 taccgccagc gaataaggac cataacgctc cacctcgtcg cggagggtcc ggatcatctg
     5881 cgaatggcac gcatagcaac cctcgcgcat gtagatgttg cgtcctgcga gctcgagcgg
     5941 cgtgtagacg cgcatgtcgg gcgcttcctc gacggtctcg tcgatggtga aaagcggcgc
     6001 gatctccacg aagccgccga tcgaggcgac gatgatgatc ccgatcgcga gggcgatcga
     6061 attcttctcg agcttgtaat gggaaccgaa catcccgctc actccccggc ttcgatggct
     6121 ctggcgggcg caagcgcagc gaccggcttg tccttcgcca tctcgtgcgc gggcgaggtc
     6181 gcctcgcgga tcgtcatgca gacgttgtag aagccgacag ccgcgcccga caggaacagc
     6241 agccctccga tcgcccgcgc gatgtagtag gggtgcatgg cgacaaggct gtcgatgaac
     6301 gaataggcga gcgtgccgct ttcattatag gtgcgccaca tcaggccctg gatgatccct
     6361 gagttccaca tcgcgagcac gtagacgagc gttccggcaa tcgcgagcca gaagtggacc
     6421 tcgaccagcg ccggcgaata catgcggggc cgcttccaca gctgcggcac caggctgtag
     6481 accgcgccga acgtgatcat cgacacccag ccgagcgtgc cggcgtgcac gtgcgcgatc
     6541 gtccagtccg tgtagtggga aagcgagttc acggcgcgga tggccatgaa cgacccctcg
     6601 aacgtcgaga ggccgtagaa cgccgccgcc accatcatga agcgcagcgt cgcgtcgtcc
     6661 cgcaccttgt cccacgcacc attgagggtg agcagcgcgt tcgccgccgc cagccaggag
     6721 ggcaccagca gcatcaccga gaacgtcatg cccagcgtct gcacccattg cggcagcgcc
     6781 gtataatgca ggtggtgaga acccgcccac atgtagaaga atgtgattcc ccagaaaccg
     6841 aggatcgaca tccggtacga gaagatgggc cggttcgcgc gcttcggcag gtagtaatac
     6901 atcatgccga ggaagccggc ggtgaggaaa aaggcgaccg cgttgtggcc gtaccaccat
     6961 tgggtcatcg catcctggac gccggagaag gccgaatagc tcttggcgct ggagaacgag
     7021 atgggaatgg cgaggttgtt gacgatgtgg agcgcggcaa cgaccaggat gaacgccatg
     7081 tagtaccagt tggcgacgta gatgtgcggc tcctgccggc gcgccagggt gcgcagatag
     7141 agcaccaggt acgtgaccca gacgatcacc agccagatgt ccgcgtacca ttcgggctcc
     7201 gcatactcct tcgactgggt gatgcccatg agatagccgc tgaccgccag cacacagaac
     7261 aagttgtagc cgatgagcac gaaccacggg ctgagctggt ccggcatccg cgcgcgcgac
     7321 gtgcgctgca tgacgtggaa ggacgtggcg atcagggcat tgccgccgaa accgaaaatg
     7381 acgccggtgg tgtgaacggg ccgcagccgc ccgaagctcg cccatccggc atcgaacgtc
     7441 aggttcggat aggcgagcag ccaggaaacc caatcgccga tgaacatcgc gaagacggcc
     7501 cagaccatcg ccaggacgat gccggctttg atggggtcgt cgtaatattg cgtgaaccgc
     7561 tcctcacccg gttcgggcgc gtcataatgg gacaggacga agaatgctgc ggcaacaccg
     7621 caaatcagca cgatgaagcc gtgaccaccg agcaaatcat cgcgaccagc cgtcgccatg
     7681 atcagacccc ataaggccac cgcgatcgag attatgagcg cgagttgccg ctcgcttctg
     7741 gtgagctgcg agaccatctc agcattgccc tctgttggac ctgtgggtct gaaatcctgc
     7801 tcgaatcact gcactctgtt cccgacccgc cggtgtgaag ccgctgcggg ccgcgcataa
     7861 gccatcatcg aaacacccgg gctcgacggc agggggcggt gacacggcac acctctcttt
     7921 tgatgatttt caggtgcatc tttttatcca agctcgcttc ttgggcaaca gctcactgcg
     7981 gagacatttg gcctcatgcc agcagcgggc gaccgtgccg gttcgtgaga aatgcttttc
     8041 gagcggaagg aagcaggatc aggcggtgaa ccgcggattt ggtcaggtcc gccgatccac
     8101 gagcgaggcg atacgggcaa ggcgcacgat gacattcgag agcgcctttt ccgcttccgc
     8161 cgataagggc cctgtcgccg acgcgaggat cgtctccagc tcctcactca gcgcttcgag
     8221 cggcttctgc gccgtgacga gcaaactcaa ccggctcttg ctcgcgccgt cgtcgacgag
     8281 cgctgcggta tgcgccggag ccggaggacc ggacagctcc tcgaggaggc gcgcgccctc
     8341 ctcgtcgcca agcttgcgaa gcttcccagc cagcgcgcga tgatggtcgg cgatcgccgc
     8401 ctcgcgttcg gcgaggaaag tgcgaagctc ctcgaccgtg ccgatcgtca atcggctgct
     8461 ccgcggcaga cccagatggt aggcgctgcg ccgccaacgc cgcagcagaa ccgcatgttg
     8521 cagcgcctcg acggcgagct tctccgccgc ggcagcgaca gccggatcgc gcgcatgcgc
     8581 cgcgagatag ctgtacatcg agaaggcccg ttccttgctc cgcaccccga tcgcaaatgc
     8641 gcggtatggt gtgacgaggg cgctgccggc gatttcgtcc catgcagccg agagctccgc
     8701 cggcagccgc cactgaaagt cctccgcagg cgggacctcc tctcccagct cgctggccca
     8761 ttgctcgacg gcggcagcat gcttgtgctc ctccgcctgc atgcgccgga atgcctgcgc
     8821 cgtcgcacgc tccccgcgcc gctccatttc gtccgcgagc cggccatagc ggcgcatcgc
     8881 atcctgctcg atcgcattcg cgatgccgac cagatcgcgc agcgtcagac gggtcaaatc
     8941 gactttcggc agcggctcga ccatcacggg ctccttcacg catggggcat cctgtcaccg
     9001 gatgaccacc cctgatttga ttcaagacaa cgtgcgcctc tgcccgaact cctatgggta
     9061 actcccgcgt cgaccgtgag acgggaaagt ccgcaacggg atgcaaacag agggactgcg
     9121 atcttgggca gaatcgtcat cattttcctg gcttggtgcc tcctcggagt gccggcttgg
     9181 agcgccgagg ggggcgggtc ggaactccag gcctatctgg cacagctctc cgcgtccgac
     9241 ctcgtaccgg gcgccgaccg tttcgggccg gtcgaaggtc agccgccgat cgcacccgtc
     9301 tatcgcggga gggaattgat cgggcatgct ttcctcaact ccgatcacgc taatgccgtc
     9361 ggctactcgg gcaagccgat tcatatcgtc gtcggcatcg ataccgaggg aacgatcacc
     9421 ggcctgaagc tcgttcacca tagcgagccg atcgtcctc
//
LOCUS       DASVDU010000092        10265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1395046, whole genome shotgun sequence.
ACCESSION   DASVDU010000092 DASVDU010000000
VERSION     DASVDU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10265
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395046"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF226_06015"
     CDS             <1..442
                     /locus_tag="VF226_06015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7073579.1"
                     /translation="YPDHQRYFTEEIAPLLDRKRRYLGPVDFARKRHLLAAAQCVVIP
                     SIEPETSSLVLREAFAAGTPVIAIRRGALAEVIDHGVTGLLVDSVAEMAAALLTTSAL
                     DRDACRKIARERFSLERMIDGYIQLYREVLRRSASQLAEFEIAP"
     gene            439..1458
                     /locus_tag="VF226_06020"
     CDS             439..1458
                     /locus_tag="VF226_06020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7073580.1"
                     /translation="MSAVLDIQTISDAGELAALAPQWWELWEHAPAATPFQSPAWLIP
                     WWQAFAPGRLMTVAVWDDNKLVALAPLYLETGPLGHRLLPIGISLGDYLDILVDSSSD
                     EALGGIGGQLSRWKEWETCELTCLAPGAAALSLPCPAGCKEAQESSDTCPVLYLSAQD
                     GLDAGTHPAIQARQRRKLRMARNRLSRGPAWSIVDTGDLAASQWLDELIRLHTTRWTE
                     RREIGVLGGECVQTFHGSALSELIDRKIARLFALRIGGEVAGIYYGFCARGRAYAYIG
                     GFDPRFAFFSPGTVLLGHAIEKALQEGIHEFHFLRGGEAYKYAWGAVDQHSIKRTFTR
                     SMIGA"
     gene            1451..2353
                     /locus_tag="VF226_06025"
     CDS             1451..2353
                     /locus_tag="VF226_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7073581.1"
                     /translation="MPKPSADTVFDALPERFRGPLEACAGRELAPNVALMRLLIEAQS
                     AEEIERAISRAKRMIGISDQLDVLSTLWLNARGAFGTVKSVLAMADHQGAWTETDWAR
                     IFDELADVSPEAGVALYSLGSPALLELATRSLVRKLNEWGLLDKTRTVLDLGCGIGRV
                     CAALASEVQAVVGIDVSVKMLEIARRRCATCSNVLLAKTSGRDLAVFADETFDLVLAV
                     DIFPYLMLAGDEVAHQHIHEARRVLKTGGSLVVFNYAYDMNPAASAEAFKRQADEAGL
                     AAVRVGTHDLDWWDGITYFARRTA"
     gene            2433..3647
                     /locus_tag="VF226_06030"
     CDS             2433..3647
                     /locus_tag="VF226_06030"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX7073582.1"
                     /translation="MPRAAVLVLDSVGIGGAPDAARYDDEGADTVGHIAERCALREGD
                     RAGLRSGPLRVPNLVSLGLGRACEIATGRIPPGLEGEALLASFGSAIETSRGKDTPSG
                     HWEIAGVPVTFDWGYFPNRKPCFPPDLVSAICKEACLPGILGDCHASGTAIIEELGEE
                     HLRTGKPICYTSVDSVFQIAAHEEAFGLQRLYDLCGIVRRLVDPLRIGRVIARPFLGR
                     SAGDFQRTPNRRDFSVSPPSPTLLDHATAAGRDVVSIGKIADIFAHSGTGRVLKGAGN
                     MAMFDCMLEGLSTLADGGLLFANFVDFDTEYGHRRDVSGYAAALEAFDSRLPELLRSL
                     RPDDLLIITADHGCDPTWKGTDHTREQVPILAYGAGRRPGPIGRRRTYADIGATAAKH
                     LGLAPLTAGEPF"
     gene            complement(3653..4747)
                     /locus_tag="VF226_06035"
     CDS             complement(3653..4747)
                     /locus_tag="VF226_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX7073583.1"
                     /translation="MCAKSIRVGIVGVGNCASSFVQGLTHYRNRNANEQPPGLMNAEV
                     GGYLVDDIEITAAFDVSAAKVGRDVADAIVAPPNNTIQFAHVPHTGIKVNRGPLLDGI
                     GKYLKDEIEVSAEPEADVTQTLRQSGTEVVISYLPVGAEMATQHYAQCAIDAGCAFVN
                     CVPVFIASDRAWRERFEKAKLPIIGDDIKSQVGATIVHRVLANLLRERGVRIDRTYQL
                     NFGGNSDFKNMLERERLESKRISKTQSVVSQFDTPPASGNVHVGPSDFVPWLTDRKWA
                     YIRLEGTTFGGVPLNMELKLEVWDSPNSAGVVIDAVRCAKLAMDRGIGGALIGPSSYF
                     MKSPPRQFPEAEARALTLAFMRGDGGYEKN"
     gene            complement(4770..5363)
                     /locus_tag="VF226_06040"
     CDS             complement(4770..5363)
                     /locus_tag="VF226_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073584.1"
                     /translation="MSRNIFLIKEGEFSIFEYSEEVFPGYLFQILVWLLEIDPQNSAF
                     SVSPYDRGTAATLLRPSPDFVMVGCGSCLCHVPLHTDASPEHRNAWQVAWFPLVAEMV
                     VEVACERLLFSASEKPEHKRPLVRSTGIFFAKQADACPHPKCARLPVRPLTADFKIER
                     HQKPRELVVWLRSRSSLEMHAARTGESLNHRMSLRPD"
     gene            5420..6538
                     /locus_tag="VF226_06045"
     CDS             5420..6538
                     /locus_tag="VF226_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073585.1"
                     /translation="MSERVLITGGGGFIGRHLARALLERGAQVRILDNLNEQVHGHIA
                     SAQALDADAEMIVGDVRDADIVERALKGIDKVVHLAAEVGVGQSMYAVERYVSANDCG
                     TAVLMQSLIDNPVERIVVASSMSIYGEGLYRDAEGALIEDMPRLARRMNGSAGWDPVD
                     SQGRPLTPVPTPEWKRPSLASVYALTKYAQEQLVMLMAAAYGMDAVALRLFNVYGSGQ
                     SLSNPYTGVLAIFASRLLNRRSPIIFEDGEQRRDFVHVGDVARAFVLALEHPNAPGNI
                     YNVGSGEHRSISDVAELLAGAMGISGIEPEITGKARAGDIRHCFADVTKIRNELGFVP
                     QANFADGLMELSRWVASQEAQDRVQKAREELEQRGLVA"
     gene            6535..7653
                     /locus_tag="VF226_06050"
     CDS             6535..7653
                     /locus_tag="VF226_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073586.1"
                     /translation="MKADGFIRGPKRTGSAARRPVLVTGGAGFIGCNLADRLAREGHD
                     VLVLDALMRPGVDVNLAWLKRRHPKRITAVIADVRDETAVAAAAREASAVFHLAAQVA
                     VTTSVDEPYRDFDVNVRGTLLLLEALRQQRDRIPFIFASTNKVYGDLGGLELERVGDA
                     YLPRAHYVRTHGVDEGQPLSLQTPYGCSKGAADQYVLDYARSFGLPTVVMRMSCIYGP
                     RQLGTEDQGWVAHFVRSTLKGEPITIYGDGCQVRDLMYVDDAVEAYLAAWRHLAVARG
                     NVLNLGGGPTNAVSLLQVVSEIGAITGRALDVSFADWRASDQRYYVSNIRRATRMLGL
                     SAPRPWRDGLRQLCEWMEATLSGSHDMDLVPMEPAELS"
     gene            7650..8942
                     /locus_tag="VF226_06055"
     CDS             7650..8942
                     /locus_tag="VF226_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HEX7073587.1"
                     /translation="MKVALVNPPWRFENSIYFGCRAPHLPLELGYTKALLEQSGHIVL
                     MLDGHLCGMDCKALAEWVAHFGPDMTVVTTAPSYLFWRCAQPELRMPKEFLLALDGRG
                     GTTVAVGPHGSSTPAAVLRKLAVDIAVMGECEEIVAQLAEGRALADVPSIAYRAGGDI
                     RINGGPHAARFVDLPALRWPAEWVTRHHHHHHRFDREPDGPGAEVEASRGCPYSCTFC
                     AKMDFRNRYRKRELSLLLEEVDALIAQGVTYVYFIDEIFLPNAELLAALEARDIRFGV
                     QTRIDLWKPEMLDALGRAGCVSIEAGVESLSPEGRDLLAKRCRMSTDELADRLIYARR
                     SIPFVQANLIKMAGDDVELIAQWRAKLERHGVWANDPVPLYPYPSSPDYYRLWGKPDD
                     HAWERSHAHYLAQFTQFSDIQDERPRPLSELEATCCRS"
     gene            8927..10081
                     /locus_tag="VF226_06060"
     CDS             8927..10081
                     /locus_tag="VF226_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7073588.1"
                     /translation="MLPELTQAGPLRVFMTTDAVGGVWQYSLDLAQGLRAYDTETTIC
                     VLGPKPQPDQLAAARRIPGVRLICLDLPLDWTASSPEVVLDAAATVVDAAADDGSSVV
                     HLNSPALAAGPRFPAPVVAVCHSCMATWWQSVRKKPLSRQFRWHRDLVAQGLGKVDAI
                     VAPSRAFALAVAEAYSLTTAPTVVYNGRRPCVRQPLPEGGISIPDEPFAFTAGRLWDE
                     GKNLGTLDAAAARLSLPVFAAGPVKGPNRTSIKLNAIKQLGNLSDGHLAEWLAAKPIF
                     VSTARYEPFGLAVLEAAEAGCPLVLSDIPTFRELWEGAAYFVPATDAAAVAAAIEELA
                     SDARQRTLYASAARRRARRYTAEVMAAKVRAIYGGLLSRKRLSAGSEVLA"
     gene            10078..>10265
                     /locus_tag="VF226_06065"
     CDS             10078..>10265
                     /locus_tag="VF226_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7073589.1"
                     /translation="MRIVYFTHSLASCWNNGNAHFLRGTLRELIALGHEAVACQPRTG
                     WSLENLLKDHGPRALDAFR"
BASE COUNT         1887 a         3208 c         3201 g         1969 t
ORIGIN      
        1 ctaccccgac caccagcgct atttcacgga agagatagca ccgcttctcg accgaaaaag
       61 acgttatctc ggcccggtcg atttcgcgcg caaacggcat cttcttgcag ccgcgcaatg
      121 cgtcgtcatt ccgagcatcg agcctgagac gagctcgctg gttctccgcg aagcatttgc
      181 tgccgggaca cccgtcattg cgatccggcg gggtgcgctg gcggaggtca tagatcacgg
      241 cgtgaccggc ctattggtgg acagcgtcgc agaaatggca gcggcacttc tgacgacgtc
      301 agccctggat cgagacgcat gccgcaaaat tgcacgcgag cgcttctcgc tggagcggat
      361 gatcgatgga tacatccaac tctatcggga ggtcctccgg cgaagcgcga gtcagcttgc
      421 agagtttgag atcgcgccgt gagtgccgtg ctcgacattc agacgatcag cgacgccggt
      481 gagcttgccg cgctggctcc gcaatggtgg gagctctggg agcatgctcc cgcggcgacg
      541 cccttccaat cccctgcctg gctgatcccg tggtggcaag cctttgctcc gggccgactg
      601 atgaccgtcg cggtttggga cgacaataag ctggtggcct tggctccgct ttacctcgag
      661 accggcccgc tcggccaccg attgctgcct atcggcattt cgctcggcga ctatctcgat
      721 attctcgtcg attcgtcctc ggacgaggcg ctcggcggaa ttggtgggca attgtcccgt
      781 tggaaagaat gggaaacttg cgaactgacc tgtctggcac ccggtgctgc cgctctttcc
      841 ctcccctgcc ccgctggctg caaggaagca caagaaagct cggacacttg cccggttctt
      901 tatctgtcag cgcaagatgg cctcgatgcc ggcacccatc ctgccattca ggcgcggcag
      961 agacgaaagc ttcggatggc gcggaacagg ctatcgcgtg gtcctgcatg gtcgatcgtc
     1021 gataccggtg atttggcggc gagccaatgg ctcgacgagc ttatccggct gcatacgaca
     1081 cgctggacgg agcggcgcga gattggcgtg ctgggaggtg aatgtgtcca aactttccac
     1141 ggcagcgcac tttcggaact gatcgatcgc aaaatcgccc ggctctttgc gttgaggatc
     1201 ggcggcgagg tcgcgggcat ttactacggc ttttgcgctc gtggacgcgc ctacgcctat
     1261 atcggcggtt ttgatccgcg gtttgccttt ttcagccctg gcacggtgct gctgggtcac
     1321 gccatcgaaa aggcactgca ggaaggaata catgaattcc atttcctccg tggcggagag
     1381 gcttacaagt acgcgtgggg cgcggtcgat cagcattcga tcaagcgaac attcacgagg
     1441 agcatgattg gtgcctaaac cctcggccga cacagtcttc gatgccctgc cggagcgctt
     1501 tcgggggccg ctcgaagcct gcgccggcag agagcttgcc ccgaatgtcg ccctgatgcg
     1561 tctcctgatc gaagcgcagt ctgccgaaga gatcgagcga gccatttcgc gtgcgaaacg
     1621 catgatagga atctccgatc aactcgacgt tctttcgact ttatggctga acgctagagg
     1681 cgccttcggc accgtcaaat ccgtgctcgc aatggccgat catcaaggcg cctggacgga
     1741 aacggactgg gccaggattt tcgacgaatt ggcggacgtc tcgcccgagg ccggtgtcgc
     1801 gctctattcg ctgggctcgc cagcgttgct cgaacttgcg acccgcagtc tggtgagaaa
     1861 gctcaatgaa tggggactgc tcgacaaaac acgaaccgtg ctcgacctcg gctgcggcat
     1921 agggagggtt tgcgccgctc ttgcgagcga ggtccaggct gtcgtcggca tcgacgtttc
     1981 cgtcaaaatg ctcgagatcg cgcggcgaag gtgcgcaacc tgctcgaatg ttcttcttgc
     2041 caagacatcc ggccgcgact tagcggtgtt cgctgacgaa acattcgacc tcgtgctggc
     2101 cgtcgacatc ttcccgtatc tcatgctcgc cggtgatgaa gttgcccacc aacacattca
     2161 cgaagcccga cgcgtgctca agacgggtgg ctcgctggtg gtcttcaatt acgcctatga
     2221 catgaatccc gccgcaagcg cagaggcgtt caagcggcag gccgatgagg cgggccttgc
     2281 agctgtccgc gtgggcacac acgacctcga ctggtgggac gggatcacct attttgcccg
     2341 ccgcacggcc tgatcgcgta aacgttcact atcgagcgat tgggtgctca ggtgcacgat
     2401 ccatgacgaa tccaatacgt gaggattaag gaatgccacg tgctgcggtt ctcgttctgg
     2461 attcggtcgg catcggcggc gcgcccgatg cggcacgtta cgacgacgaa ggtgcggata
     2521 cggtcggcca catcgccgag cgctgcgcgc tcagagaagg agaccgcgcc ggtttgaggt
     2581 ctggaccact gcgcgtgccc aatctggtca gcctcggact cggccgggcg tgcgaaattg
     2641 cgacaggccg catcccgcca gggcttgaag gcgaggctct tctggccagc ttcggctctg
     2701 ccatcgaaac ctcccgcggc aaggataccc catccggtca ttgggagatc gccggagtac
     2761 cggtcacctt cgactggggt tacttcccga ataggaaacc gtgcttcccg cccgatctgg
     2821 tgtcggccat ttgcaaggag gcctgcctgc ccggaatact cggcgactgc catgcttccg
     2881 gcacggcgat catcgaggag ctgggcgaag aacatctgag aacgggaaag ccgatctgct
     2941 acacgtccgt agacagcgtt ttccagatcg ccgctcacga ggaggcgttc gggcttcaac
     3001 gcctttatga cttgtgtggg atcgtgcgcc gcctggtcga tccgctacgg atcgggcgcg
     3061 tgattgcccg tcctttcctc ggtcgctcgg ccggcgactt ccagcgcacg ccgaaccggc
     3121 gcgacttctc cgtgtccccg ccgtcgccga cgcttctcga tcacgccacc gctgccgggc
     3181 gggatgtcgt gagcatcggt aaaatcgcgg atatcttcgc ccattccgga accggtcgcg
     3241 tgctcaaggg agcgggcaac atggccatgt tcgattgcat gctggaagga ctttcgacgc
     3301 tcgccgacgg tgggctgctg ttcgccaact tcgtcgattt cgataccgag tacgggcatc
     3361 gtcgcgacgt ctcgggctat gccgccgcgc tggaggcctt cgattcccgc ttgccggaac
     3421 tgctccggtc tctccgcccc gacgatctgc tgatcatcac cgccgaccac ggctgcgatc
     3481 ccacctggaa gggaaccgat cacacgcgtg agcaggtacc catcctcgca tacggtgccg
     3541 gccgcagacc tggcccgatc ggccgccgcc gaacctacgc tgatatcggc gcaacggccg
     3601 cgaaacacct gggactggcg cccctcaccg cgggagagcc gttttgaggc agtcagttct
     3661 tttcgtagcc gccgtcgccg cgcatgaacg cgagcgtcag cgcgcgcgct tccgcctctg
     3721 gaaactgccg cggcggtgat ttcatgaaat agctcgacgg cccgatcaat gctccgccga
     3781 tgccgcgatc catcgccagc ttggcgcacc tgacggcgtc gatgacaaca ccagcggaat
     3841 tcggcgagtc ccagacttcg agcttcagct ccatgttgag cggaacgccc ccgaaagtcg
     3901 tcccctcgag tcgaatgtag gcccatttgc gatccgtgag ccacgggacg aaatcgctcg
     3961 gcccaacgtg cacgttcccg cttgccggcg gggtgtcgaa ttgactgacg acggattgcg
     4021 ttttcgaaat tcgcttcgat tcgagccgtt cgcgctcgag catgttcttg aaatccgaat
     4081 tgcccccgaa attcagttga tacgttcggt cgatccgaac gccgcgctcg cgcaacaggt
     4141 tggccaggac acgatgaacg atcgtggcgc cgacctggct cttgatgtcg tcacctatga
     4201 tcggcagttt tgccttctcg aaccgctcgc gccaagcgcg gtcggaggca atgaagaccg
     4261 gaacacagtt cacgaacgcg cagcccgcat cgatagcgca ctgcgcgtaa tgctgagtgg
     4321 ccatttccgc gccgaccgga agataggaga tgaccacctc cgtgccgctc tggcgcaacg
     4381 tctgggtaac gtcggcttca ggctcggccg aaacttcgat ttcgtctttg agatacttgc
     4441 cgatgccgtc cagaagagga ccgcgattaa ccttgattcc cgtatgaggg acgtgcgcga
     4501 actggatcgt attgttgggc ggcgcgacga tcgcatctgc gacatcgcgg cccaccttgg
     4561 ccgcactaac gtcgaaggca gcggtgattt cgatgtcgtc cacgagatac ccgccgacct
     4621 ccgcattcat caatccgggc ggctgctcgt tggcattgcg gttgcgataa tgggtcagtc
     4681 cctggacgaa agaggacgca cagttcccaa ccccgacgat ccccacgcga atactcttgg
     4741 cgcacaaggc ggcaaactcc gtaactcggt taatcggggc gcaaactcat gcgatgattg
     4801 agggattcgc ctgttctcgc ggcatgcatc tcaagcgaac ttctcgatct caaccacacg
     4861 accagctctc gtggtttttg gtgccgttcg attttgaagt ccgctgtcaa tggccggacg
     4921 ggaagtctgg cgcacttcgg atggggacac gcgtctgctt gcttggcgaa aaatatgccg
     4981 gttgaacgca cgagcggccg tttgtgttcc ggcttctctg aagcggaaaa cagcaaccgc
     5041 tcgcacgcca cttcaaccac catctcagct accaaaggga accaagccac ctgccatgcg
     5101 tttcgatgct caggcgacgc atcggtgtgg agaggcacgt ggcacaggca agaaccacaa
     5161 ccgaccatga cgaaatccgg agatgggcgg aggagcgtgg cggccgtccc tctatcgtaa
     5221 ggactgacgg agaaggcgga attctgcgga tcgatttcca ggagccagac gagaatctgg
     5281 aagagatatc ctgggaagac ttcttccgaa tattcgaaga tagaaaactc gccttccttt
     5341 atcaggaaga tatttcggga cacaaaagcc gattcaataa gctcgtcgat cgcaacgcgt
     5401 aagacgagag attagagaaa tgtctgaaag agttctgatc accgggggcg gtggcttcat
     5461 cgggcgtcat ctcgcgcggg cgcttctgga acgcggcgcc caggtccgca tcctcgacaa
     5521 tctcaacgaa caagtccacg gccacatcgc cagcgcgcag gcgctcgatg ccgatgcgga
     5581 gatgatcgtc ggcgacgtgc gcgatgcgga catcgtggaa cgggcgctca agggcatcga
     5641 caaggtcgtg catctcgccg ctgaagtggg cgtcggccag agcatgtacg cggtggagcg
     5701 ttacgtcagc gccaacgact gcggcacggc cgtgctcatg caatcgttga tcgacaatcc
     5761 ggtcgagcgc atcgtcgtcg cctcgtccat gagcatctat ggcgaaggcc tctaccggga
     5821 cgccgaaggc gctctgatcg aagacatgcc gagactcgcg cgccgtatga acggcagcgc
     5881 ggggtgggac cccgtggaca gccagggccg accgctcacc ccggtgccga cgcccgaatg
     5941 gaagcggccg agcctggcgt ccgtatacgc gctgaccaag tacgcccagg agcagctggt
     6001 gatgctgatg gcagccgcct acgggatgga cgccgtggcg ctccggttgt tcaacgtcta
     6061 cggctcgggc cagtcgcttt ccaatccata taccggcgtg ctcgccatct ttgcatcgcg
     6121 cctcctcaac cggcggagcc cgatcatttt cgaggacgga gagcagcggc gcgatttcgt
     6181 ccacgtcggc gatgtcgctc gggcgttcgt tctggcgctg gaacacccga acgcacccgg
     6241 caacatctac aacgtcggca gcggcgagca tcgctccatt tctgacgtcg cagaattgct
     6301 cgcgggtgcc atggggatca gcggaatcga accggaaatc accggcaagg cacgcgccgg
     6361 cgatatccgt cactgcttcg ctgatgtgac gaaaatccga aacgagcttg gtttcgtgcc
     6421 gcaggcgaac tttgccgacg ggctcatgga gctcagccgc tgggtcgcca gccaggaggc
     6481 ccaggacagg gttcagaagg cgcgggagga actggagcag cgaggattgg tcgcatgaaa
     6541 gcggacggct tcatccgcgg gccgaaaagg acgggctccg ccgcgcgccg gccggtcctg
     6601 gtgacgggcg gcgcggggtt catcggctgc aatctcgctg accggcttgc acgcgagggc
     6661 cacgatgtcc tcgttctcga tgcgctgatg cggcccggcg tcgatgtgaa cctcgcctgg
     6721 ctcaagcgac ggcatcccaa gcggattacc gcggtgattg ccgacgtgcg cgacgagacg
     6781 gcggtcgcgg cggcagcccg tgaagcgagc gcagtttttc atcttgctgc ccaggtcgct
     6841 gtcacgacca gcgtcgatga gccctatcgc gatttcgacg tcaacgtccg gggcacgctt
     6901 ttgctgctgg aggccttgcg gcaacagcgc gatcgcattc ctttcatttt tgcgagcacg
     6961 aataaagtct atggcgacct cggcggcctg gagctggagc gcgtcggcga cgcctatttg
     7021 ccgcgtgcac actatgtgcg cacccacggc gtggatgagg gccagccgtt atcgctgcaa
     7081 acgccgtacg gctgctcgaa gggcgcagcg gatcagtacg tgctcgatta cgcccgcagc
     7141 ttcggcctgc cgacggtcgt catgcgcatg agctgcatct atgggccgcg ccagctcgga
     7201 accgaggacc agggctgggt agcccatttc gtccgatcga ctctgaaagg cgaaccgatc
     7261 acgatctacg gcgacggctg ccaggtgcgc gacctgatgt atgttgatga tgccgtcgaa
     7321 gcctatctcg ccgcctggcg tcatttggcc gtcgcgcgcg gcaatgttct caacctcgga
     7381 ggtggaccga ccaatgcggt cagcctgctg caggtcgtct ccgagatcgg ggctataacc
     7441 ggacgagctc tcgacgtcag cttcgcggac tggcgcgcca gcgaccagcg ctactacgtc
     7501 tccaatatcc gcagagccac acgcatgctg ggcttgagcg cgccgcggcc ttggcgcgat
     7561 ggcctccgcc aactctgcga gtggatggaa gcgaccttgt ccggatctca tgacatggac
     7621 ttggtcccga tggagcctgc ggagctgtca tgaaggtcgc cctcgtcaat cccccgtggc
     7681 gtttcgagaa cagcatctat ttcgggtgcc gcgcaccgca cctgccgctc gagcttggct
     7741 acacgaaggc gctgctcgag cagagcggcc atatcgtgct gatgctcgac ggccatcttt
     7801 gcgggatgga ttgcaaggca ttggccgagt gggttgctca cttcgggccg gacatgacgg
     7861 tcgtgacgac cgcaccgagc tatcttttct ggcgctgcgc gcaacccgag cttcggatgc
     7921 cgaaggagtt cctgctggcg ctcgacggac gaggcggaac gacggttgcc gttggtccgc
     7981 acggctcgtc aaccccggca gcagtgcttc gcaagctcgc cgtggatatc gccgtcatgg
     8041 gcgagtgcga ggaaatcgtg gcgcagctcg ccgaggggcg tgcactcgca gatgtcccct
     8101 ccatcgccta tcgcgcaggc ggcgatatcc ggatcaacgg tgggccgcac gcagccagat
     8161 ttgtcgattt accggcgctg cgctggccag ccgagtgggt gacgcgccat caccatcatc
     8221 accatcgctt cgatcgcgag cccgacggac ccggtgccga agtggaggcg tcgcgcggtt
     8281 gcccctattc ctgcacgttt tgtgcaaaaa tggacttccg caaccgttac cgtaagcgcg
     8341 agctcagcct gctgctcgag gaagtcgatg ccctgatcgc ccagggcgtc acctacgtct
     8401 acttcatcga cgagatcttt ctgccgaacg cggaattgct ggcagcactc gaagcgcgcg
     8461 atatccgctt cggcgtccag acccggatcg acctctggaa accagagatg ctcgatgcgc
     8521 tcggccgggc aggctgcgtt tcgatcgagg ccggcgtcga aagcctcagt ccggaaggac
     8581 gcgatctgct ggccaagcgg tgccggatgt ccacggacga gcttgccgat cgcctgattt
     8641 acgcacgccg ctcgataccg ttcgtacagg cgaacctgat caagatggcg ggagacgatg
     8701 tcgagctgat cgcgcaatgg cgcgccaagc tcgagcggca tggcgtgtgg gccaatgatc
     8761 ccgtcccgct ttacccttat ccgagctcgc ccgactacta ccggctctgg gggaaacccg
     8821 atgaccacgc gtgggagcgt tcgcacgccc actatctcgc ccagttcact cagttcagcg
     8881 acatccagga cgagcggccg cgccccttat ctgaattgga ggcgacatgc tgccggagct
     8941 gacacaggcg ggcccattgc gggtgttcat gacgaccgac gcggtgggtg gcgtctggca
     9001 gtactctctc gatcttgccc aagggctcag ggcctacgac accgagacga ccatctgcgt
     9061 cctcggtccc aaaccgcagc ccgaccagtt ggcggcggcg aggcgaattc cgggcgttcg
     9121 tctcatttgt ctcgatctgc cgctcgattg gacggcatcg agccctgagg tcgtgctcga
     9181 tgcggcggcc acggtcgtcg atgcggcggc cgatgacgga agcagcgtcg tccacctcaa
     9241 cagcccggca ctcgccgcgg gtccgcgatt tcccgcgccg gtcgtggcgg tctgccactc
     9301 gtgcatggcc acctggtggc aatccgtgcg caaaaaaccc ctttcacgcc aattccgctg
     9361 gcacagggat ctcgtcgcgc aaggccttgg caaagtcgat gcgatcgtcg ctccgagccg
     9421 agccttcgcg cttgccgtag cggaagccta tagcctgacg accgcaccga ccgtcgttta
     9481 caacgggcgt cggccctgtg tgcgccagcc cttgccagag ggcgggatca gcattcccga
     9541 cgagccgttc gcgttcacgg ccggtcgcct gtgggatgaa ggcaagaacc tggggacttt
     9601 ggacgcagcg gccgcacggc tttcactgcc ggtcttcgcc gccgggccgg tcaaggggcc
     9661 aaaccgtacg agcatcaagc tcaatgccat caagcagctg ggcaatctga gcgacgggca
     9721 tcttgccgaa tggctcgccg ccaaaccaat tttcgtgtcg acggcccggt acgagccatt
     9781 cgggcttgcc gtgctggaag cggccgaagc gggttgcccg ctcgtgctct ccgacatccc
     9841 gaccttccgc gagctgtggg aaggagcggc atatttcgtg cctgcgaccg atgccgcagc
     9901 ggtcgccgcc gccatcgagg agctcgccag cgatgctcgc cagcggacgc tttatgcctc
     9961 cgcagcacga aggcgcgcgc ggcgctatac ggccgaggtc atggctgcga aggtccgcgc
    10021 catctacggc ggcctgctgt cgaggaagcg cctcagcgcc ggcagcgagg tcctggcatg
    10081 aggatcgttt atttcacgca ttcgcttgcc tcgtgctgga ataacggaaa tgcgcatttt
    10141 cttcgtggca cgcttcgtga gctgatcgcg ttgggtcacg aagcggtggc ctgccagccg
    10201 cgcaccggct ggagcctcga aaatctgctc aaagatcacg ggccgcgcgc tctcgatgcg
    10261 ttccg
//
LOCUS       DASVDU010000093        21046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_230412, whole genome shotgun sequence.
ACCESSION   DASVDU010000093 DASVDU010000000
VERSION     DASVDU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21046
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230412"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2525)
                     /locus_tag="VF226_06070"
     CDS             complement(120..2525)
                     /locus_tag="VF226_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7073590.1"
                     /translation="MDSARPDTVDPKTLAILRELERKVLWLSTYMIHHANHEVDRGDG
                     LKVGGHQASSASLATLMTALYFHVLRPEDRVAVKPHASPVFHAIQYLLGNQSLDKLQN
                     FRGFAGAQSYPSRTKDKDDVDFSTGSVGLGVAMTGFASLIQDYVHAKWNPAWPKGRMV
                     ALMGDAEIDEGNVHEALLEYWKHGLTNAWWVVDYNRQSLDSVVSDRLFMKVAGLFENL
                     GWKVVLLKYGKLLQQVFARPGGEDLRRWIDDCPNQLYSALVFQGGAAWRKQLESDLAG
                     CEDALEIVRNHSDTELARLMTNLGGHDLETILETFAAAPTGQPVCFIAYTIKGVGLPF
                     QGHKDNHAGLMTKAQMQEFRASQGINQGEEWDRFAGLSLPPEEIQAFLDRVPFAKRGR
                     RRLSAARVPVPELPIPESRGPVSTQAGFGRILDDIAKQGGPLADRIVTTSPDVTVSTN
                     LGPWVNRRAVFSMQSVEDVFKERGLMSPQKWEMSPKGQHIELGIAEMNLFLTLAAAGL
                     SHSLFGERLIPIGTLYDPFVCRGLDALNYACYQDARFIVVGTPSGITLAPEGGAHQSI
                     STSLIGMAQDGLASFEPAFVDELAVIMNWAFDYVQREGEEEKEERWLRDEKGGSVYLR
                     LTTRPIEQLQRQITPELRSEIIDGGYWLRRPDPGTSLAIIYTGAVAPEAIEAAGLLSE
                     DRRGVGVLAVTSADRLSAGWHGAQRAREHGARGARSHVENLLAALPRDAAIITICDAH
                     PETLSWIGSVMGHRVRPLGVEHFGQSGSISELYGHYGLDVNAILAAAEGLGARPVRYR
                     IVS"
     gene            complement(2539..3798)
                     /locus_tag="VF226_06075"
     CDS             complement(2539..3798)
                     /locus_tag="VF226_06075"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX7073591.1"
                     /translation="MQGRVEKPVENAESAAQAVSLADVEKAAEVLKGAIVETDCDPSR
                     TLSSIAGCDIWLKFENLQFTASFKERGALNRLNALAPEQRAAGVIAMSAGNHAQGVAY
                     HAKRLGIPATIVMPKGTPFVKIENTRRHGAQVIVDGATLEESAACARAFGQQHGLTFI
                     HPYDDPLIIAGQGTLALEMLRSAPDLATLVVPIGGGGLISGMAVAAKAIKPGIEIIGV
                     QAELYPSMYNKIRGTELPMRGDTLAEGIAVKAPGALTEALVRRYVDDIMLVSERDIEH
                     AAALLIEIEKTVAEGAGAVGLAAVLRDPARFRGRKVGLVLSGGNIDTRLLASVLTREL
                     ARAGRISELCIDLADKPGQLARLATLVGDAGANIIEVVHQRVFTDLPAKGTLLRMVIE
                     TRDRAHLEDTLARIRAAGFAAEITSNQ"
     gene            4055..4450
                     /locus_tag="VF226_06080"
     CDS             4055..4450
                     /locus_tag="VF226_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073592.1"
                     /translation="MMKSLAIATATIAFSGAALLADSAPVQAATAAAKNAEISVAARI
                     HRNPSRVQASAVSRGVLLPYVIKKATAQRKAINNWNAKVRRMYGPQYASWNRAKAKST
                     TCNRVASAVTCRVSAIPTSNFRRYGMLND"
     gene            4611..5495
                     /locus_tag="VF226_06085"
     CDS             4611..5495
                     /locus_tag="VF226_06085"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073593.1"
                     /translation="MAKVAWIGLGVMGYPMAGHILKKGGHDLVVYNRTTAKAEEWVRE
                     YGAGRAAKTPAEAAADADFVFCCVGNDDDLRSVTIAENGAFHGMKKGAIFIDNTTASA
                     NVARELYAAAKEKGFDFLDAPVSGGQAGAQNGVLTVMVGGDQPVFERAQPVIANYARM
                     VTLIGPVGSGQLTKMVNQICIAGLVQGLSEGIHFAQKAGLNIPAVMETISKGAAGSWQ
                     MENRWKTMSEGKFDFGFAVDWMRKDLMISLDEARRNGASLPVAALVDQFYSEVQKMGG
                     GRWDTSALIARLNNADKK"
     gene            5698..8010
                     /locus_tag="VF226_06090"
     CDS             5698..8010
                     /locus_tag="VF226_06090"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7073594.1"
                     /translation="MGPIVPAWAFLPQRRGQPSGKRRLGLGRIAGLLMLAALVAFRVW
                     DPPLLATLRGKIFDTYQRLHPRIATHYPVTIVDIDERSLQAIGQWPWPRTILASLVDR
                     LAARGAVAIGFDILFAEPDRLSPHRLAEAIPDPELKSRLGTLPDSDAQLAEAMARTRV
                     VVGQAGAASRSAAQAEPDNLPVTSVATIGENLQRHLLQVPQLVPNVPAFEKAAAGRGL
                     FTVEPDADGVIRHIPTLLFAEGRIVPALSIELLRVATGATTLLAKSDEAGARSVVVAG
                     VEIETERDGQLWLHFTPHDPQRYVSAVDVLEGRVPEDRIEGKLVLIGTSAAGLFDMRA
                     TPLEPAMPGVEIHAQAVENILDNALLLRPNYALGLEVSLAIAIGIGIVVIVPLLGALP
                     TVLLGVLTAGLLAALSGYLFVSQKLLLDVVYPLVSSTVVFLLLASINYVREEKRRAGI
                     RSAFSQYLAPELVEQLTREPDRLKLGGETRTMSVLFSDVRGFTSIAESFKDNPSGLTE
                     LMNRLLTPLSRAVIERRGTIDKYIGDALMAFWNAPLDDPDHALNACEAALAISDRLDE
                     LNAALALEAQRGGGSVPAMEIGIGISTGTCMVGNMGSDIRFDYSVLGDSVNLASRIQA
                     LAANYGLRILVCPTTAERCRDRLAIIEVDDVRVRGRRDVVTIHTVLGGPAYRQQSGFT
                     VFQTAFTEMRAHYRRCEWDRAISAHESCRMHSGDDPRLDRLLDIYASRIAILKGRSPP
                     KEWHGVYSDAFGSPPGPASAAKDLVKSIHG"
     gene            8003..8800
                     /locus_tag="VF226_06095"
     CDS             8003..8800
                     /locus_tag="VF226_06095"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HEX7073595.1"
                     /translation="MGKSIMIGARTELAVILLAIVIIGLPIPLHPSSANAAHPEIGST
                     VAVKNRVTAELADEKRRLLKGAKVHQDEILVTGAKSSAEIKLLDDTKLAVGPSARLVL
                     DKFVYDADAPPKSISVTLAKGAFRFISGTSAKAAYEIRTPTATMGVRGTVFDVFVADN
                     GESVVLLHEGSVDVCAGPSNCRRHDVIGKIMHIGLGGLLSEPANWNRSLIRGIRVTRA
                     FPFVGRRLAIDPVRRLTHSALSRGRVDDVIRKPLDGLRELRRQLPFR"
     gene            8946..9563
                     /locus_tag="VF226_06100"
     CDS             8946..9563
                     /locus_tag="VF226_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7073596.1"
                     /translation="MFIPVELYKVLETLGWVAMALTLCSTFSRTIIRLRVFAAYSNVL
                     AIVSSIAAGYWPNAVQNAIQLPLNVQRIREARKQVDKLKAAPVTGVHSEWLSPHARES
                     SLSAGQVLFRKGDPGDRLYYLVSGSVLFDEINVEIGPGTLFGEIAFFTRDGCRTQTAT
                     ATSDCKLLSIDGDQLKEIYFQNPEFGWYLIQLIAQRLMAPTERTP"
     gene            9711..10760
                     /locus_tag="VF226_06105"
     CDS             9711..10760
                     /locus_tag="VF226_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX7073597.1"
                     /translation="MRSKRVITVVGCHAEGEVGDVITGGVLPPAGATMLEKKLTMERE
                     HDHIRRMLIQEPRGSVARHVNLIVPPTRKDAHAGAIIMEPTEYPPMSGSNTMCITTVL
                     LETGMVPMQEPETRLRLDMPGGPVDVVAECRDGKCLSVTISNVPSFAAKLDAPLEIEG
                     HGTIHVDIAYGGMFYAIADARALGFAVEPHEARDLAILGEKIRIAAREQHEIVHPEFP
                     DIRGVSIVQFACPFAEPNAVVRNTCIVSPGRSDRSPTGTGTSARMAALHARGLLEVGQ
                     GITHESIIGSRFEGRIVGTTTVNGTPAVLTTIKGRAWITGIHEYFVDPSDPWPQGYVV
                     SDTWGTTNTTLQPSE"
     gene            10875..13103
                     /gene="purL"
                     /locus_tag="VF226_06110"
     CDS             10875..13103
                     /gene="purL"
                     /locus_tag="VF226_06110"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX7073598.1"
                     /translation="MDASAPAAETAFRITPELVAEHGLKPDEYERLLSILGREPTLTE
                     LGIFSVMWSEHCSYKSSRVWLRQLPTSGPQVIQGPGENAGVVDLGDGWCAVFKMESHN
                     HPSYIEPYQGAATGVGGIMRDVFTMGARPIANLNALRFGDPSHPKTRHLVSGVVAGIG
                     GYGNCVGVPTVGGETNFDPGYNNNILVNAMCVGLARKDRIFYSAAKGVGLPVVYVGSK
                     TGRDGIHGATMASAEFDENSEEKRPTVQVGDPFTEKLLIEACLELMAEDAIIAIQDMG
                     AAGLTSSTSEMADKGGVGIELDLDNVPQREEGMTAYEMMLSESQERMLMVLKPGAEAK
                     AEAIFRKWGLDFAIIGQTTDTGRMVVRHKGRVEADIPVSKLANSAPVYERPWVPVEPP
                     KSVAAKDVPAPNAIMDALRTMMGHPQLCSRRWIWEQYDYMVMGHTVQRPGGDAAIVRI
                     PGTTKGIAMACDVTPRYVAADPDMGTRQAVAENWRNLTAVGADPLAITDNMNFGNPER
                     PEIMGQFVQSVKGMAEACRALDYPVVSGNVSLYNETNGVGIPPTPAIGGVGLVPDLSK
                     TASLALKKEGDVLILIGREAGHLGQSLYQQIIAGQLQGAPPPVDLADEIKAGRLIRAL
                     IRESGVRTVHDVSDGGLLVAVAEMALAGDLGLELFEYQGKLPAHAVWFGEDQGRYVVE
                     VSPDEAEAVIERARSLALPARIVARVGGDSLALPGEPPVKLAELRDIHESWLPHYMSG
                     TH"
     gene            13276..13512
                     /locus_tag="VF226_06115"
     CDS             13276..13512
                     /locus_tag="VF226_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX7073599.1"
                     /translation="MAMDAKEIERLIKASLPDAEVSIRDLAGDGDHYAAHVVSSAFVG
                     KTRVQQHQMVYEALGGRMGGALHALALTTSPRKD"
     gene            13593..13943
                     /gene="grxD"
                     /locus_tag="VF226_06120"
     CDS             13593..13943
                     /gene="grxD"
                     /locus_tag="VF226_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX7073600.1"
                     /translation="MTDQAVHDWIAKTVATNDVVLFMKGTSTFPQCGFSAQVAQILGH
                     LGVDYKDVNVLEDMGIREGIKTYSNWPTIPQLYVKGEFIGGCDIVREMFQQGELQRLF
                     DDKGVSYDKKSAVV"
     gene            14341..14970
                     /locus_tag="VF226_06125"
     CDS             14341..14970
                     /locus_tag="VF226_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX7073601.1"
                     /translation="MIEWRIEGRELVSCNCIVGCPCQFNAPPTHGFCDAVVAIQIDQG
                     RFGETPLDGLRAVAVMTWPGAIHEGHGKCQVIIDERASEAQREALLKILSGQETEPGA
                     TIFNVFAATFEQVFEPLFKPIEIDIDVDARRGSVRVDGMVQVTGSPILNPVTGSEHRA
                     RIDMPDGFEFRIAEVGRASTQASGNLAFGFDDTHAHFARLNMSQNGVAA"
     gene            15035..15850
                     /locus_tag="VF226_06130"
     CDS             15035..15850
                     /locus_tag="VF226_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX7073602.1"
                     /translation="MIDAVTRTEALLRRERLIIIAGLAIIIALAWTYIISGGGTAMTA
                     VSMTHWRLPLSMAMHAAPATWTLTYALVMVTMWWTMMIAMMLPSAAPTVLLYAQVTRH
                     AARQGQVNATVPAAVFVAGYLAVWLAFSILATGLEWLLERAGVIDGMMMGSVDAWLSG
                     GLLIAAGLYQLSPLKGVCLDHCRAPAVYLSQHWRAGRSGAFLMGIGHGAYCLGCCWAL
                     MLLLFVGGVMNLLWIAGLTAVVLLEKLSPFGSRLAPWLGSGFLLGGTLVILLV"
     gene            complement(15925..16542)
                     /gene="rpsD"
                     /locus_tag="VF226_06135"
     CDS             complement(15925..16542)
                     /gene="rpsD"
                     /locus_tag="VF226_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7073603.1"
                     /translation="MTKRVSAKHKIDRRLGENIWGRPKSPINKRESRPGQHGERRMGK
                     LSDYGQQLRAKQKLKGYYANITERQFRRIYDTAARLKGSTSELLIGLLERRLDAIVYR
                     AKFVPTPYAARQFISHGHVKVNGRRVNIASYRCKVGDVVEVREKSRQIPMLLEAIKSP
                     ERDVPDYIEVDHNKMTAKLLRVPALSDVPYPVIMEPNLVVEFYSR"
     gene            complement(16739..17326)
                     /locus_tag="VF226_06140"
     CDS             complement(16739..17326)
                     /locus_tag="VF226_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073604.1"
                     /translation="MGKIWTAGMRAAALGLGALMMVLTPANAQDISERAVKAFMDYAW
                     SLTPAKFTKPDGTTVEIDKKDRSKVEVPLDTAREVIKVGRLTAHAQICELPEDQVANY
                     RSLMRREEAKGKWTEQQLIYINQLHLTTVMLLTGKIRLVEKTDGGKEVVIDEKEAPDA
                     NTCTDEQRKKVKELIAAYVATGPDLAERSTGSVKK"
     gene            complement(17430..18722)
                     /locus_tag="VF226_06145"
     CDS             complement(17430..18722)
                     /locus_tag="VF226_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX7073605.1"
                     /translation="MSRADERTAGPQMRAVNPKHAKTQSPAIEETDLTVAEVVGAAYR
                     ENVEAASALAEPPLSPEEVRAAINYCADEACVAAQAFCPGCRKWTESQGLATLDQFCA
                     RFSSISFADTGLVLSPGGPLPAATFPSLDALAKTWAGEELWFLARRVIRRLKRLEDPR
                     PKRLAGIDEDGLPSPTLILVRPQLADNIGMVARAMANFGLDDLRLIAPRDGWPNEKAR
                     AAAAGANFIIDATRASGTLAEAVADLNFVCATTARRRDLAKPVLTPEQAAQEIHRRIE
                     EGERCGIVFGPERAGLETDEVALADAVVMAPVNPRFASLNVAQAVLLVSYEWLKETKR
                     GTLGRVTSYEKGLEPGLNRRGARPATKEELYGFFDHLESELDRLGFFNPPHRRQTVMR
                     NVKSMFARMNASEQEVRTLRGIVATLAHGKGAGRKPPA"
     gene            complement(18805..19725)
                     /locus_tag="VF226_06150"
     CDS             complement(18805..19725)
                     /locus_tag="VF226_06150"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7073606.1"
                     /translation="MPHNRRVLDREFWRKRRRRFAARAVTRATGLIGASPRLRGLALR
                     ALLDQHLAPGALCLVPFGDHALYVDPRDDKIALKLMGGRCWQRRELETAIGALRRAGQ
                     FRMGGVFIDVGANIGALSIYALKSGLFSEGIAIEPDPHNFAILKRNLSLNGFEQRVRA
                     IEAAASRGAGRLQLVRNSKNHGAHSVERSFRSGRGEGIEVEALTLDDILAQEGVDADR
                     VSLVKIDVEGHELAVLDGMSRLLDARVPILVELTADRDDVERLQRFKALLSPHYDRVL
                     DLSDGIENAQPSRSLCDLTWRTSQADLLIC"
     gene            complement(20233..>21046)
                     /locus_tag="VF226_06155"
     CDS             complement(20233..>21046)
                     /locus_tag="VF226_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7073607.1"
                     /translation="SFGLRLKRYPKPEIKARVEEAARILDITELLDRKPKQLSGGQRQ
                     RVAMGRAIVRHPKVFLFDEPLSNLDAKLRVQMRTEIKKVHQKVRTTTVYVTHDQVEAM
                     TLADRVVVMNQGRIEQVGSPNELYHKPATRFVASFIGSPAMNFIPCELEESDGKLAVR
                     LTGDLALPIPEARRERYRSLKGNGRLLLGLRPEHITEVRPHMEPHVVPFEAQIEVTEP
                     MGMETLVYFSINGTQVCGRVNPNANAREGAPMRLAADLGNMHLIDDETGRVV"
BASE COUNT         3673 a         6857 c         6681 g         3835 t
ORIGIN      
        1 gctaaatcga gctttgcggg cgtgcgcccg agccagggtg cacgcctcgt tttctgtcca
       61 actgagcgga ccgtcgttgt aaaattcatt ccgcgcaacg ggcgccggaa tgtcagaccc
      121 taactcacga tccgataccg aacaggccgg gcgcccaagc cttccgcggc ggcaaggatg
      181 gcattcacgt cgagcccata gtgaccgtag agctcgctga ttgagcctga ttgaccgaag
      241 tgctcgacgc cgagcggccg gacgcgatgc cccatgacgc tgccgatcca ggacaatgtc
      301 tccgggtggg cgtcgcagat ggtgatgatg gcggcatcgc gcggcagcgc ggcgaggagg
      361 ttctcgacat gcgagcgggc gccgcgggcc ccgtgctctc tcgcgcgctg ggcaccgtgc
      421 cagcccgcgg aaagcctgtc ggccgaggtg acggcaagca caccgacgcc acggcgatcc
      481 tccgacagca ggcctgccgc ctcgatcgct tccggcgcga ccgcgcccgt gtagatgatg
      541 gcaaggctgg tgccggggtc gggacggcgc aaccaataac caccgtcgat gatctcggat
      601 cgcagctccg gcgtgatctg acgctgaagc tgctcgatcg gacgggtcgt caggcgcagg
      661 tagacggagc cgcccttctc atcgcgcagc cagcgctctt ccttctcctc ctcgccctcg
      721 cgctgaacgt aatcgaatgc ccagttcatg atcaccgcga gctcgtcgac gaaggctggc
      781 tcgaacgagg ccaatccgtc ctgggccatg ccgatcagcg acgtcgaaat ggactggtgt
      841 gcaccgcctt ccggcgccag cgtgatgcct gacggcgtcc cgaccacgat gaagcgcgcg
      901 tcctgatagc aggcgtagtt cagggcatcg aggccgcggc agacgaacgg atcgtagagg
      961 gtgccgatcg ggatcagccg ctcgccgaac agcgagtgtg acagccccgc cgctgcgagc
     1021 gtgaggaaca ggttcatctc ggcgatgccg agctcgatgt gctgtccctt gggcgacatc
     1081 tcccatttct gcggagacat cagcccacgc tccttgaaca catcctcgac gctctgcatg
     1141 gagaagacgg cacgccggtt gacccacggc ccgaggttgg tcgagacggt gacgtcgggg
     1201 gaggtcgtga cgatacgatc ggccagcggc ccgccctgct ttgcgatgtc gtcgaggatc
     1261 cgcccgaacc cggcctgcgt cgaaaccggg cccctgctct ccggaatggg aagctccggc
     1321 accggcacgc gcgccgcgct cagccgccgc cggccacgct tggcgaatgg gacgcggtcc
     1381 aggaaggcct ggatttcctc gggcggcaag gacagacccg cgaagcggtc ccactcctcg
     1441 ccctggttga tcccttggct cgcgcggaac tcctgcatct gggccttggt catcaggccg
     1501 gcgtgattgt ccttgtgccc ctggaacggc aatccgacgc ccttgatcgt gtaggcgatg
     1561 aagcagaccg gctgaccggt cggcgcagcg gcgaacgtct ccagaatggt ctcgaggtcg
     1621 tgcccgccga gattggtcat caggcgggcg agctcggtat ccgagtggtt ccggacgatt
     1681 tccagcgcat cctcgcagcc ggcgagatcg gactcgagtt gcttgcgcca cgccgcgccg
     1741 ccctggaaca cgagtgccga gtagagctgg ttcgggcagt cgtcgatcca tctgcgcaaa
     1801 tcctcgccgc caggtcgcgc gaaaacctgc tgcagcagct tgccgtattt cagcagaacg
     1861 accttccagc cgagattctc gaacagcccg gcgactttca tgaacaggcg gtccgacacg
     1921 acggaatcga ggctttgccg gttgtagtcg accacccacc aggcgttggt gagcccgtgc
     1981 ttccagtact cgagcagggc ctcgtggacg ttgccctcgt cgatctcggc atcgcccatc
     2041 agcgccacca tacggccttt gggccaggcg ggattccact tggcgtgcac gtaatcctgg
     2101 atgaggctcg caaaccccgt catcgcgacg ccgaggccga cggagcccgt cgagaagtcg
     2161 acgtcgtcct tgtccttggt ccgtgacgga tagctctggg cacccgcaaa gccgcggaaa
     2221 ttctgcagtt tgtccagcga ctgattgccc agcaggtact ggatggcatg gaacaccggg
     2281 ctcgcgtgcg gcttcaccgc gacgcgatcc tccggccgca gcacgtggaa atagagcgcc
     2341 gtcatcagcg tcgccagcga ggcggaggag gcctgatgac cgcccacctt gaggccgtcg
     2401 ccgcggtcca cctcatggtt ggcgtggtgg atcatgtagg tggagagcca tagcacctta
     2461 cgctccagct cccgcaggat cgccagtgtc ttcgggtcca ccgtgtcggg ccgggcgcta
     2521 tccatgagct gccttctcct actgattcga cgtgatttcc gcagcgaagc cggccgcgcg
     2581 gatacgggcc agcgtgtcct ccaggtgggc acggtcgcgc gtctcgatga ccatccgcag
     2641 cagcgtgccc ttggcaggaa gatccgtgaa aacgcgctgg tgcacaacct cgatgatgtt
     2701 cgcacccgca tcaccgacga gggtggcaag ccgggcaagt tggccgggtt tgtcggcaag
     2761 gtcgatgcaa agctcggaga tgcgtccggc ccgggccagc tccctggtca ggacgcttgc
     2821 gaggagccgc gtgtcgatat tgccgcccga gagcacgaga cccaccttgc ggccccggaa
     2881 ccgcgccgga tctctcaaga cggcggcaag cccgacggcc cctgcgcctt cggcgacggt
     2941 tttctcgatt tcgatcaaca gcgccgccgc gtgctcgatg tcgcgctcac tgaccaacat
     3001 gatgtcgtcg acgtatcggc ggaccagcgc ctcggtcagc gcgccgggcg ccttcaccgc
     3061 gataccctcg gcgagcgtgt cgccgcgcat gggcagctcg gtgccacgga tcttgttgta
     3121 catcgacggg tagagctcgg cctgaacgcc gatgatctcg atcccgggtt tgatggcctt
     3181 cgccgcgacg gccattcccg agatgaggcc gccgccgccg attggtacga cgagcgtggc
     3241 gaggtccggc gcggatcgca gcatctccag cgcgagggta ccctgcccgg cgatgatgag
     3301 cgggtcgtcg tagggatgga tgaaggtcag cccatgctgc tgcccgaagg cgcgcgcaca
     3361 ggcggccgat tcctcgagcg tcgcgccatc gacgatcacc tgggcgccgt gccgccgcgt
     3421 gttctcgatc ttcacgaacg gcgtgccctt cggcatgacg atagtcgcag gaattccgag
     3481 tcgcttcgcg tgatacgcga cgccttgcgc atgattgccg gcggacatgg caatgacccc
     3541 ggcggcccgc tgctccggcg cgagggcatt cagccggttc agcgcgccgc gctccttgaa
     3601 ggaggcggta aactgcaggt tctcgaactt cagccagatg tcgcagccgg cgatggacga
     3661 aagcgtgcgg ctcgggtcgc aatcggtctc gacgatcgca cctttgagca cctcagccgc
     3721 cttttcgaca tctgcgaggc tcacggcttg cgccgcgctc tcggcattct cgacgggctt
     3781 ttcgactctg ccttgcatcg ctttagaatc gtcctttgtc tcacctgacc ggaagctaat
     3841 cgcccacgag ttcgacaatc gcgaatgcag gctcaacgcg cgttccttcg attgctgctt
     3901 aacaccgtcg cgcgccttgc gaaacggtta cgcgcccatg aacgctcgtt gaatgcaccg
     3961 ttcagcatca cctcagcccg cagagtctag ctttggaacc gttgaaaggg tccgcgcaca
     4021 ggccgcggct cgataaacga aaaggagcta gatcatgatg aagtctctcg ccatcgccac
     4081 ggcaaccatc gctttcagtg gtgcagctct tctggccgac agcgccccgg tccaggcggc
     4141 gacagcggcc gcgaagaatg ccgagatcag cgtagcggcc cgcattcacc gcaacccgtc
     4201 ccgtgtgcag gcttcggccg tatcgcgcgg cgtcctgctc ccctacgtga tcaagaaggc
     4261 gacggcgcaa cgcaaggcga tcaacaactg gaatgccaag gttcggcgca tgtacggtcc
     4321 gcaatacgcg agctggaacc gcgccaaggc gaaatcgacg acctgcaacc gcgtcgcatc
     4381 cgccgtgacc tgccgcgtca gtgccattcc aacctcgaac ttccgccggt acggcatgct
     4441 gaacgactga cgccggcaat agcaagcctc tgtgacctgc aacggggagc cgctggctcc
     4501 ccttctcttt tcgcacgaat gtcggccatc cgggcggcag acctgacaac gcgccatcca
     4561 tgggttagaa actcggtcca atcccaagag cgaacgcatg gagaggcaag atggcgaagg
     4621 ttgcgtggat cgggctcggc gtgatgggct atcccatggc cggccacatc ctgaaaaagg
     4681 gcggtcatga tctcgttgtt tataatcgca ccaccgccaa ggccgaggaa tgggtgcggg
     4741 agtacggcgc cggccgcgcg gcgaagacac cggccgaggc tgctgcggat gcggacttcg
     4801 tgttctgctg cgtcggcaac gacgacgacc tgcgctctgt gaccattgcc gagaacggcg
     4861 ccttccacgg catgaagaag ggcgcgatct tcatcgacaa caccacggcc tccgccaatg
     4921 tcgcgcgcga gctctacgcc gcggccaagg aaaaaggctt cgacttcctc gacgctcccg
     4981 tttccggcgg ccaggccggt gcgcagaacg gcgtgctcac cgtcatggtc ggcggcgacc
     5041 agcccgtgtt cgagcgggcc cagccggtca tcgccaacta cgcccgcatg gtgaccctga
     5101 tcggcccggt cggctcgggc cagctcacca agatggtgaa tcagatctgc atcgccggac
     5161 tggtccaggg gctttctgag ggcatccact tcgctcagaa ggcgggcctc aacattcccg
     5221 ccgtcatgga gaccatctcc aagggcgcgg cgggctcctg gcagatggag aaccgctgga
     5281 agacgatgag cgaaggcaaa ttcgatttcg gcttcgccgt cgactggatg cgcaaggacc
     5341 tcatgatcag cctcgacgag gcgcgccgca acggcgccag cctccccgtg gcggcgctgg
     5401 tcgatcagtt ctattccgag gttcagaaga tgggcggcgg ccgctgggat acgtcagccc
     5461 tcatcgcgcg cctcaacaac gccgacaaga agtagctcac gacccgtccc gtgcggtgca
     5521 ctgctgttag ccgtgcccgc acgcccctca ttgtcccctt gccttcccaa gatccggcgg
     5581 cgtctgcaga tcgtcgctag catagctccg actcgaaagc tcctgcacca agactcaaat
     5641 cccgctagtg cgaaccgtta ggacgctgct aaatgacagt tgtcggaggt tcgtggggtg
     5701 gggcctatcg tgccggcttg ggcattcctg ccgcagagac gtggtcaacc gtcgggaaag
     5761 cgtagactcg ggctggggcg tattgccggc ctgctgatgc tcgccgcgct ggtcgcgttc
     5821 cgggtctggg atccgccgct gctcgcaacg ctgcgcggaa aaatctttga tacgtatcag
     5881 cgccttcatc cgcgcatcgc cacgcactat cccgtcacga tcgtggatat cgatgaacga
     5941 tcgctgcagg ccatcgggca gtggccgtgg ccgcgtacga ttctagcgtc cctcgtcgat
     6001 cgcctcgcgg cccgtggcgc cgttgcgatc ggcttcgaca tccttttcgc cgagcccgac
     6061 cggctgtcgc cgcaccgcct ggcagaggcg atacccgacc ccgaactgaa atcgcggctc
     6121 gggacgctgc cggacagcga tgcgcagctc gctgaggcca tggcccgcac ccgagtcgtc
     6181 gttggacaag ccggggcagc gagccgttca gccgcgcaag ccgagcccga taatctgcca
     6241 gtcacgtcgg ttgcaacgat cggagaaaat ctccagaggc acctcctgca ggtgccgcag
     6301 ctggtgccga acgtgccggc tttcgagaag gccgctgccg ggcgcggact gttcacggtc
     6361 gagcccgacg cggacggcgt catccgccac atacccaccc tgctctttgc cgaggggcgc
     6421 atcgtccccg cgctctcgat cgagctgtta cgtgtggcga ccggtgccac cacacttctc
     6481 gccaagagcg acgaggccgg agcgagaagt gtcgtcgttg ccggcgtcga gatcgagacg
     6541 gagcgcgatg ggcagctctg gttgcatttc acaccgcacg acccgcagcg ctacgtctcc
     6601 gccgtcgacg tgctcgaggg ccgggttccc gaggatcgca tcgagggcaa gctggtgctt
     6661 atcggcacct ctgccgccgg cttgttcgac atgcgcgcaa cgccgctcga acccgccatg
     6721 cccggcgtcg agatccacgc ccaggccgtc gaaaacatcc tggacaacgc gcttctgttg
     6781 cgcccgaact atgcactggg gctcgaagtc tctttggcga tcgccatcgg tatcggcatc
     6841 gtcgtcatcg ttccgttgct cggcgcgttg cctacggtcc tcctgggtgt gctcaccgct
     6901 ggactgctcg cggccctatc cggctatctc ttcgtcagcc aaaaacttct gctcgatgtg
     6961 gtttatccgc tggtgtcgag cacggtggtg ttcctgctgc tcgcttccat caattacgtc
     7021 cgggaggaga aacggcgggc cggcatccgc agtgccttca gccaatatct ggcgccggag
     7081 ctcgtggagc agctgacgcg agagcccgac cggctgaagc ttggcggcga gacccggacg
     7141 atgtccgtgc tcttcagtga cgtgcgcggc ttcacctcca tcgcggaaag tttcaaggac
     7201 aatccgtccg ggctgaccga gctgatgaac cgcctgctga cgccgctgtc ccgagccgtc
     7261 atcgagcgcc gcggcacgat cgacaagtac attggcgacg ccctcatggc gttctggaac
     7321 gcaccgctcg acgatcccga tcacgccctc aacgcgtgcg aagccgcgct tgcgatctcc
     7381 gatcggcttg atgagctcaa cgccgcgctc gcgctcgaag cccagcgcgg cggcggaagc
     7441 gtgccggcca tggagatcgg catcgggatc agcaccggca cctgcatggt cggcaacatg
     7501 ggctccgata tccggttcga ctattcggtg ctcggcgaca gcgtcaacct ggcatcacgg
     7561 atccaggccc tcgcggccaa ctatggtctg cgcatcctgg tctgtcccac aacggccgag
     7621 cggtgccgcg acaggctggc gatcatcgag gtcgacgatg tccgcgtcag gggacgcagg
     7681 gacgtggtga cgatccatac tgtccttggc ggccccgctt accggcagca atccgggttc
     7741 accgtgttcc aaaccgcctt cacggaaatg cgtgcacact accggcggtg tgagtgggac
     7801 cgggcaatca gcgcccacga aagctgccgg atgcacagcg gcgacgatcc gaggctggat
     7861 cgcctgctgg atatctacgc ctcgcgcatt gcgattttga agggccgctc acctccgaaa
     7921 gagtggcatg gcgtctattc ggacgcgttc ggatcgccgc cgggaccggc ttccgcggcc
     7981 aaggacttgg tgaagagcat tcatgggtaa gagcatcatg atcggagcca gaactgaact
     8041 tgcagtgatc ctactggcca tcgttatcat cgggctgccg atccccttgc atccatcgtc
     8101 tgccaacgcg gcgcatcccg agatcggcag caccgttgcg gtcaagaacc gggtcacggc
     8161 ggagctggcg gacgagaagc gccggctctt aaaaggcgcc aaggtccacc aggacgagat
     8221 tttggtaacg ggcgcgaagt cgagcgccga aatcaagctg ctcgacgaca ccaagctggc
     8281 cgtcggtcca tcggcgcggc tcgtgctgga caagttcgtc tatgatgccg atgcgccgcc
     8341 gaaatccatc tccgtcaccc tcgccaaggg cgctttccga ttcatcagtg ggacgagcgc
     8401 caaggcggct tatgaaatca gaacgccgac ggccaccatg ggggtgcgcg gcacggtttt
     8461 cgacgtcttc gtcgcggata atggagaatc ggtcgtgttg ctgcacgaag gcagcgtcga
     8521 cgtctgcgcc ggtccctcca actgccggcg gcacgacgtg atcggcaaga tcatgcacat
     8581 cggcctcggc ggtctgctgt cggagccggc caactggaat cgctcgctga tcaggggcat
     8641 cagggtcacc agagcctttc cgttcgtggg aagacgactg gcgatcgacc ccgttcgacg
     8701 gctcacgcac tcagccctct cgagagggag ggtcgacgac gtgatcagaa agccgctgga
     8761 tggcttgcgc gaacttcgcc ggcagctccc gttccgctga gcacttgttc agaataggtc
     8821 gctccgaaag gtaacccgct cggagacgat tttctcgcct cgcttcgcct aacttcccga
     8881 ctcactgaga tttttccggt gccggtcggt atcggtctcg ggtttgccaa agaggatgat
     8941 gcgaattgtt cattccagta gagctgtaca aggtgctgga gaccctcgga tgggtcgcca
     9001 tggcgctcac tctctgctcc acattttcgc gaacgatcat ccgactgcgt gtgtttgcag
     9061 cctacagcaa tgttctcgcc attgtctcgt ccatcgctgc cggctactgg ccgaatgcgg
     9121 tccagaatgc cattcagctt ccgctcaacg tccagcgcat tcgcgaggcg cgcaaacagg
     9181 tcgacaaatt gaaggccgcg cccgttacgg gggtgcactc ggaatggctt tccccgcatg
     9241 ctcgcgaaag ctctctgtcg gccggacagg ttttgttccg gaagggcgat ccgggcgatc
     9301 ggctctacta cctcgtgtca ggcagcgtcc tcttcgatga gatcaatgtt gaaatcggcc
     9361 ctgggacatt gttcggcgag atcgcgttct ttacccgcga cggatgccgg acccagaccg
     9421 ccacggcgac atcggactgc aagctcctgt ccatcgacgg cgatcagctg aaggagatct
     9481 atttccagaa cccggagttc ggctggtacc tgatccagct cattgcccag cggctgatgg
     9541 cgccgaccga gaggacccca tgatcaaacc acccctgccg cggcgctggc tgacggttac
     9601 tcccgaggcg cagtgactgg gcggtggggt cagggggacc tcggccttca tagccgtcca
     9661 atttgccgat aagaataaga attggacgac gaagcagcgg agacatccgg atgcgcagca
     9721 agcgcgtgat caccgtggtc ggctgtcacg ccgagggcga ggtgggcgat gtcatcactg
     9781 gcggcgtgct gcccccggcc ggcgcgacga tgctcgagaa gaagctcacc atggagcgcg
     9841 agcacgatca catccgccgc atgctcattc aggagccgcg cgggtcggtg gcccggcacg
     9901 tcaacctgat cgtgccgccg acgcggaaag acgcccacgc gggcgcgatc atcatggagc
     9961 cgaccgaata cccgcccatg tcgggctcca acacaatgtg tatcaccact gtgctcctcg
    10021 agaccggcat ggtgccgatg caggagccgg agacgcgact gcgcctcgac atgccgggtg
    10081 ggccggtcga cgtggtcgcc gagtgccggg acggcaaatg cctcagcgtg acgatctcca
    10141 acgtgccctc cttcgcggcg aagctcgacg cgccactcga gatcgaggga catggcacca
    10201 tccacgtcga catcgcctac ggcggcatgt tctacgcgat cgctgatgcg cgtgccctcg
    10261 gttttgccgt cgagccgcac gaggcgcgcg atcttgccat tctcggcgag aagatccgga
    10321 ttgccgcccg cgagcagcac gagatcgtcc atcccgaatt tcccgacatt cgcggagtct
    10381 cgatcgttca gttcgcctgc ccgttcgccg agccgaacgc ggtcgttcgc aacacctgca
    10441 tcgtctcacc gggccgctcc gaccgaagtc cgacgggcac gggcacatca gcccgcatgg
    10501 cggcgctcca cgcgcgcggg cttctcgagg tcggacaggg catcacgcac gagtcgatca
    10561 tcggctcgcg gttcgagggc cgcatcgtcg ggacgacgac ggtgaacggc accccggcgg
    10621 tgctgaccac catcaagggg cgcgcctgga tcaccggcat ccacgagtat ttcgtcgacc
    10681 ccagcgatcc ctggccgcag ggctatgtcg tctccgacac ctggggcacg acaaacacca
    10741 cgttgcagcc aagcgaataa tgaatggcga actgataggg gaataggctc atagcgacgc
    10801 cttggagcgg cgacatcgcc gtccgcgccc gctataagct cgctgactgc aaaatccccg
    10861 agccaagcag gactatggac gccagcgccc ccgccgccga aaccgccttc cggatcacgc
    10921 ccgagctcgt cgctgagcac ggcctgaagc cggacgaata tgaacgcctg ctcagcattc
    10981 tcggccgcga gccgacgctg accgagctcg gcatcttctc ggtgatgtgg tcggagcact
    11041 gctcctataa gtcctcgcgc gtgtggctga ggcagctgcc gacttcagga ccgcaggtca
    11101 tccaagggcc gggcgagaac gcgggcgtgg tcgatctcgg cgacggctgg tgcgccgtct
    11161 tcaagatgga gagccacaac cacccgagct atatcgagcc ctaccagggc gcggcgaccg
    11221 gcgtcggcgg catcatgcgc gacgtgttca ccatgggcgc gcggccaatc gcgaacctta
    11281 acgcgctgcg cttcggcgat ccctcgcacc ccaagacgcg ccatctcgtg tccggcgtcg
    11341 tggccggcat cggcggctat ggcaactgcg tcggcgtgcc caccgtcggc ggcgagacca
    11401 acttcgaccc cggatataac aacaacatcc tcgtcaacgc catgtgcgtc ggcctcgccc
    11461 gcaaggaccg catcttctac tcggcggcga agggcgtcgg cctgcccgtc gtctacgtcg
    11521 gatccaagac cgggcgcgat ggcatccacg gcgcaaccat ggcctccgcc gagttcgacg
    11581 agaactcgga ggaaaagcgg cccaccgtcc aggtcggcga cccgttcacc gagaagttgc
    11641 tgatcgaagc gtgcctggag ctgatggccg aggacgccat catcgccatt caggacatgg
    11701 gcgctgcagg cctcacctcc tccacctcgg agatggccga caagggcggc gtcggcatcg
    11761 agctcgacct cgacaacgtc ccccagcgcg aagaaggcat gacggcttac gagatgatgc
    11821 tctcggaaag ccaggagcgc atgctcatgg ttctgaagcc gggggccgag gccaaggccg
    11881 aagccatttt ccgcaagtgg gggctcgatt tcgcgatcat cggccagacc accgacaccg
    11941 gccgcatggt cgtcaggcac aagggccgcg tggaggcgga tattcccgtc tccaagctcg
    12001 ccaactcggc acccgtttac gagcggccgt gggttccggt cgagccgccg aagtcggtcg
    12061 ctgcgaagga cgttccagcg ccgaacgcaa tcatggacgc gctgcgcacg atgatgggcc
    12121 atccgcagct ctgctcccgc cgctggatct gggagcagta cgactacatg gtcatgggcc
    12181 acacggtgca gcggccgggc ggcgatgccg ccatcgtgcg catccctggc accaccaagg
    12241 gcatcgcaat ggcctgcgat gtcaccccgc gctacgtggc tgccgatccc gacatgggca
    12301 cgcggcaggc tgtcgccgag aactggcgca acctgaccgc cgtcggcgcc gatccgctcg
    12361 ccatcaccga caacatgaac ttcggcaacc ccgaacggcc ggagatcatg gggcagttcg
    12421 tccagtcggt gaaaggcatg gccgaggcgt gccgggcgct ggactatccc gtcgtgtcgg
    12481 gcaatgtctc gctttacaac gagaccaacg gcgtcggcat tccgccgacg ccggccatcg
    12541 gcggcgtagg gctcgtaccg gatctttcca aaaccgccag cctagccctc aagaaggagg
    12601 gcgacgtcct catcctgatc gggcgcgaag cgggtcacct cgggcagagc ctctaccagc
    12661 agatcatcgc gggccaactc caaggcgccc cgccaccggt cgatctggct gacgagatca
    12721 aggccggcag gctcattcgc gcgctcatcc gggagtcggg cgtgcgcacg gttcatgatg
    12781 tcagcgacgg cgggctgctc gttgcggtcg cggagatggc gctcgccggt gaccttggtc
    12841 ttgagctctt cgagtaccaa ggcaagctgc ccgcccacgc ggtctggttc ggcgaggatc
    12901 aaggccgcta cgtcgtcgag gtctcgcccg acgaggcgga ggccgtgatc gagagggcgc
    12961 gcagtcttgc cctgcccgcc cgcatcgtgg cgcgcgtcgg cggcgacagc ctcgcccttc
    13021 ccggcgaacc gccggtaaag ctcgccgagc tgcgggacat ccacgagagc tggctgccgc
    13081 actacatgag cggcacacac taaggctgtg gcgggccagg ttcttgcaat gttcgcgcga
    13141 gtgagctaag cgtggacgac ccaaagcgag cgctagcatt gcagccactc gatccgcatg
    13201 cgagtgcgtc gagtggacaa gaccggatcg cggcagcgcc gttgcagctc gtcgccactg
    13261 gggagaggtt ctcacatggc tatggacgcg aaagagatcg agcggttgat caaggcgagc
    13321 cttcctgacg cggaggtgag tatccgcgat ctcgccggcg acggcgacca ttacgctgcg
    13381 cacgtcgtct cgtcggcgtt cgtgggcaag acacgggttc agcagcatca gatggtttac
    13441 gaggcgctcg gtggccgcat gggtggcgca ctgcacgcgc tggccttgac gacatcgccg
    13501 cggaaggatt gacaccgcgc ggcttgcgcc cccatgttct gtcggcgtca aggcgccctt
    13561 gaaccgccct actgacgaga ggaattcatt caatgacaga ccaagccgtt cacgactgga
    13621 tcgcaaagac ggtcgcgacc aatgacgtcg tgcttttcat gaagggcacg tcgacgttcc
    13681 cgcagtgcgg gttctccgcc caggtcgccc aaatcctcgg tcacctcggc gtcgactaca
    13741 aggacgtgaa cgtgctcgag gacatgggca tccgcgaggg tatcaagacc tacagcaact
    13801 ggcccacgat cccgcagctc tacgtgaaag gcgagttcat cggcggctgc gacatcgtcc
    13861 gcgagatgtt ccagcaaggc gaactgcagc gcctgttcga cgacaagggc gtgagctacg
    13921 acaagaaatc cgcggtcgtc tgaggatccg taccggtccc cgttggggct ttttctcaag
    13981 ccttacaatc attccagctc tgggcgcggt aagctgtgct gccgcgccct cccgtttgcc
    14041 gttcccatga gctcacagag aacccgctag acgctacgcg agcgtcttcg cccatccggg
    14101 taagtctgcc cgaatgagtc cgcccgaatg gaagcatatc cgctgctctc agctgccagg
    14161 taaggttgat gcgggcgacc ccttctcctg gacccccgcg acccgccatt tcctctgacc
    14221 ctctcagcct ctatccccgg gccgatgttt ctcgacagaa gcctggccgg tatgtatgct
    14281 gaacttcata attctgatcg ccgcagtagg cgaattgacg ccattcaaag ggaggcgtcc
    14341 gtgatcgagt ggaggatcga agggcgggag ctcgtcagct gcaactgcat cgttggttgt
    14401 ccgtgccagt tcaacgcacc accgacccac ggtttctgtg atgcggtcgt cgccattcag
    14461 atcgaccagg gtcgcttcgg cgaaacccca ctggacgggt tgcgtgcggt cgcggtcatg
    14521 acctggccgg gagccatcca tgaggggcat ggcaagtgcc aggtcatcat cgacgaacgc
    14581 gcttcggagg cacagaggga ggcgctattg aagatcctgt cgggccagga gactgagccc
    14641 ggggcgacca tcttcaacgt gttcgccgcc acgttcgagc aggtattcga gccgctgttc
    14701 aagcccatcg agatcgacat cgacgtcgat gcccgccgtg ggtctgtgcg cgtagatggg
    14761 atggtccagg tcacgggatc tccaattctc aatccggtta caggcagcga gcacagggcg
    14821 cgcatcgaca tgccggatgg cttcgagttc cggatcgctg aggtgggccg cgcaagcacg
    14881 caagcgagcg gcaacttggc tttcggcttc gatgataccc acgcgcactt cgcgcggctc
    14941 aacatgtccc agaacggcgt ggcagcctga cgtgatgatg acggctctcg cgctcgggac
    15001 tgcaacctag ctctccatcc ggccaggggc aggcatgatc gacgcggtca ccaggacaga
    15061 agccctgctc cggcgtgaac ggctgatcat catcgcgggt ctggccatca tcatcgcgct
    15121 cgcgtggacc tacatcatct cgggcggcgg tacggccatg acggccgtct cgatgacaca
    15181 ctggcggctt ccgctgtcga tggcgatgca cgccgcgccg gccacgtgga cgctgaccta
    15241 tgcgctcgtc atggtcacca tgtggtggac gatgatgatc gccatgatgc tcccaagcgc
    15301 ggcgccgacg gtcctgctgt acgcccaagt gacccgacat gccgcgcgcc agggtcaggt
    15361 caacgcaacc gttcctgcgg ccgtattcgt ggcggggtat ctcgcggttt ggctggcatt
    15421 cagcatcctg gcgaccggcc tcgagtggct gctggagcgt gctggagtga tcgacggcat
    15481 gatgatgggc tccgtcgacg cttggctctc aggcggcttg ttgatcgcgg ctgggctcta
    15541 ccagttgtcg ccgctgaagg gcgtctgtct cgatcactgc cgcgcgcccg ccgtctacct
    15601 ttcacagcac tggcgggcgg gacgcagtgg tgccttcctg atggggatcg ggcacggggc
    15661 ctactgcctc ggctgctgct gggcgctgat gctgcttctg ttcgttggcg gtgtcatgaa
    15721 cctgctttgg atcgcggggc tgacggcggt ggtgctgcta gaaaaactca gccccttcgg
    15781 ttcccggctg gcgccgtggc tcggatcggg ctttcttctc ggcggcacac tcgtcatcct
    15841 gctggtctga gtggccgacc tctcgaacgc aaaagagccg ccatctctgg cggctcccgc
    15901 gatcggcagg aagttggctc ggcgtcagcg cgagtagaac tcgaccacca ggttgggctc
    15961 catgatgacc gggtacggga cgtcggagag agccggcacg cgtagcagct ttgccgtcat
    16021 cttgttgtga tcgacctcga tgtagtcggg gacgtcgcgc tccgggctct tgatggcctc
    16081 gagcagcatc gggatctggc gcgatttctc acgcacctcc acgacatcgc cgaccttgca
    16141 gcggtagctc gcgatgttga cacggcgccc gttcaccttg acgtggccgt gactgatgaa
    16201 ctggcgggct gcatagggcg tcggcacgaa cttggcgcgg tacacgatgg cgtcgagccg
    16261 ccgctcgagc aggccgatga ggagctcgga ggtcgagccc ttgaggcgcg ccgccgtgtc
    16321 gtagatgcgg cggaactgac gctcggtgat gttggcgtaa tagcccttca gcttctgctt
    16381 ggcacgaagc tgctggccgt agtccgacag cttgcccatg cggcgctcgc cgtgctggcc
    16441 ggggcgactc tcgcgcttgt tgatggggct cttcggacgg ccccagatgt tctctccgag
    16501 gcggcgatcg atcttgtgct tggcgctgac gcgcttcgtc atgtgtaggc actccagttg
    16561 agagctgttg cagcttggag cgccccctcc tctgcccggt gtcccgagcc gacaggcgcc
    16621 gacctgactg gccgtcgccg cgggtgcgca aatcaaacgc gggcctagtg gcccgcgcgc
    16681 ggatgctcaa tatccggatt gccggcgtcc gtcaaccggc tggaaggcgc accgcctgct
    16741 acttctttac tgaacccgtt gatctttcgg ccaaatccgg acccgttgcc acgtaggcgg
    16801 caatcagctc cttcaccttc ttacgctgct cgtcggtgca ggtgttggcg tcgggcgctt
    16861 ccttttcatc gatgacgact tcctttccgc cgtccgtctt ttcgaccagc ctgatcttgc
    16921 ccgtcagcaa catcaccgtc gtcaggtgga gctggttgat gtagatcaac tgctgctcgg
    16981 tccactttcc ctttgcttcc tcccgccgca tgagcgagcg gtagttggca acctgatcct
    17041 caggaagctc gcagatctgg gcatgggccg tgaggcggcc caccttgatg acctcgcgcg
    17101 ccgtgtcgag cggcacttcg accttgctgc ggtccttttt gtcgatctcg acggtggtgc
    17161 catcgggctt ggtgaacttg gccggcgtga gcgaccaggc ataatccatg aatgccttga
    17221 cggcgcgctc gctgatatcc tgcgcgttgg ctggtgtcag caccatcatc agtgccccga
    17281 gtcccaacgc cgcagcccgc atccccgcag tccagatctt gcccattcac tcagcccttg
    17341 aagccgcctc agttgcggtc cgcaacacgt ccgtcctccc acagcattgt gcggtggttc
    17401 ggctcgaaag tgggcataat caaggcactt tatgcgggtg gtttccggcc cgcgcccttg
    17461 ccgtgagcca gcgtggccac tatgccacgc agcgtccgga cttcctgctc gctcgcgttc
    17521 atgcgggcga acatgctttt cacgttccgc atcacggtct gacgccggtg tggcgggttg
    17581 aagaagccca gtcggtcgag ctcggattcg aggtgatcga agaagccgta gagctcctcc
    17641 ttggtcgccg gacgcgcgcc tctcctgttg agacccggct cgagcccctt ttcgtaagag
    17701 gtgacccggc cgagcgttcc gcgtttcgtc tctttcagcc actcgtagct cacgagcagc
    17761 acggcctggg cgacattgag agatgcgaag cgcgggttga ccggcgccat gacgaccgcg
    17821 tccgccagcg cgacctcgtc cgtctcgagc ccggcccgct ccggcccaaa gacgattccg
    17881 cagcgctcgc cctcttctat tcgcctgtgg atttcctgcg cggcctgctc gggcgtcagg
    17941 accggctttg ccaagtcccg ccgccgcgct gtcgtggcgc agacgaagtt gaggtcggcg
    18001 acggcctcgg cgagcgtgcc cgacgcgcgc gtcgcatcga tgatgaaatt cgcacccgcc
    18061 gcggcggcgc gcgccttctc gttgggccag ccgtcgcggg gggcgatgag ccggagatcg
    18121 tccaggccga agtttgccat ggcgcgggcc accatgccga tgttgtcggc aagttgcggc
    18181 cggacgagga tgagcgtcgg gctcggcaag ccatcttcgt cgataccggc aaggcgcttg
    18241 gggcgtgggt cctcgagccg cttcagccgc cggatgaccc gccgggcaag aaaccagagc
    18301 tcctccccgg cccatgtctt ggcaagggcg tcgagggacg gaaacgttgc cgctggcagc
    18361 gggccgccgg gcgacagcac gaggccggtg tcggcgaatg aaatggatga gaaccgggcg
    18421 cagaactggt cgagagtcgc gagcccttgg ctctctgtcc acttgcggca gcctggacag
    18481 aaggcctgag cggcgacaca ggcctcatcg gcgcagtagt tgatggcggc ccgcacctcc
    18541 tcgggcgaaa gcggcggctc ggccagtgcg ctcgccgcct ccacgttctc ccgataggca
    18601 gcgccgacga cctcggcgac cgtgagatcc gtctcctcga tggcaggaga ttgcgttttg
    18661 gcgtgctttg ggttgacggc gcgcatctgc gggcctgctg tcctctcgtc ggcgcggctc
    18721 atccgggctc gtgagggctg gcgtaacgaa tgccgccgga gagagcaacc gggccgccgg
    18781 tcacaggcgt atgggcccgc gtgctcagca aatcaacagg tcggcttgag aggtgcgcca
    18841 cgtgagatcg caaagtgatc ggctcggttg agcgttctcg ataccgtccg agaggtcgag
    18901 cacgcggtca taatgggggc tcagcaaagc cttgaaccgc tgcaaccgct ccacgtcgtc
    18961 gcgatcggcc gtgagttcga ccaggatcgg cacacgcgca tccagaagac gactcatgcc
    19021 gtcgaggact gcgagctcgt ggccttccac gtcgatcttc accagggaaa cccgatcggc
    19081 gtcgacacct tcctgcgcga gaatgtcgtc gagcgtcaac gcctctactt cgatgccttc
    19141 gccgcggccg gaacggaagc tgcgctcgac cgaatgggcg ccgtggttct tgctgttcct
    19201 gacaagctgg aggcgaccgg cgccccgact ggcagcggcc tcgatagccc gcacacgctg
    19261 ctcgaagccg ttcagtgaca ggttgcgctt gagaattgcg aaattgtgcg gatcgggctc
    19321 gatggcgatg ccttcggaaa acaggccgga cttcagggca tagatgctca gtgcgccgat
    19381 attcgcgccg acgtcgatga acacgccgcc catccggaat tggccggccc tccgcagggc
    19441 gccaatcgcg gtttcaagct ccctgcgttg ccagcagcgt ccgcccatca gcttcagggc
    19501 gatcttgtcg tcgcgtggat cgacgtagag cgcatggtcg ccgaatggga ccaggcaaag
    19561 cgctccaggt gccaagtgct gatcgagcag cgcgcggagc gcaaggccgc gcagcctcgg
    19621 actggcaccg atcaatcctg ttgcgcgcgt gacagcacgc gccgcaaacc ggcggcgtcg
    19681 cttccgccag aattcgcgat ccagcactcg acgattgtgt ggcaacgaag ctctcgtgac
    19741 ggactgcagt catgcagtca acgatcatga ggcctattgg ttcagggcgt tatcaccttc
    19801 cgccgatcac aacccctcga ccgactcttg ttcaaatccc ttgtcatctc ggcgcttgtc
    19861 gccgggatcc agctcacgtc ttgctccagt gcttacggat ggctggatcc cgggcacaag
    19921 gcccgggatg actgtgtagt ggcgaagaat tctagactga aagagcagag aaccggcatt
    19981 gtccccgggg tctcaccagc tccgcgatgg tcgcctcatc gagcgaatgg ggcggcagcg
    20041 agcgtgataa ttcctcttcg ggttgattac gccgggccgg ccgccctttg gctcaaaacc
    20101 atccgtcatc ccggcgctcg tcgccgggat ctacctcatg tcttgctcca gagcttacgg
    20161 atggctggat cccgggcaca aggcctggga tcgctgcctc tatcggcaga aaattctatg
    20221 ctgaatagag tctcacacca ctcgcccggt ctcgtcgtcg atcaggtgca tgttgccgag
    20281 atcggcggcc agtcgcatgg gggcgccttc gcgggcgttg gcgttcggat tgacgcgtcc
    20341 gcagacctgc gtgccgttga tcgagaagta gacaagagtc tccatcccca tcggctcggt
    20401 gacttcgatc tgggcctcga acggcacgac gtgaggctcc atgtgcgggc gtacctcggt
    20461 gatgtgctcg ggccggagcc ccagcagaag ccggccgttg cccttgaggg atctgtaccg
    20521 ctcccgccgg gcttccggga tcggcaaagc gagatcacca gtcagtcgaa cggcgagctt
    20581 accgtcgctc tcctccagct cgcagggaat gaagttcatg gccggtgacc cgatgaagct
    20641 cgcgacaaac cgcgtcgccg gcttgtgata gagctcgttc ggcgagccga cttgctcgat
    20701 gcgtccctga ttcatgacca ccacgcgatc ggccagcgtc atcgcctcga cctgatcgtg
    20761 tgtgacgtag accgtcgtcg tgcgcacctt ctgatgcacc ttcttgattt cggtgcgcat
    20821 ctgcacacgc agcttggcat cgaggttcga cagtggctcg tcgaacagga acaccttagg
    20881 atgccggacg atggcgcggc ccatggcgac gcgctggcgc tggccacccg aaagctgctt
    20941 gggcttgcgg tcgagcagct cggtgatgtc gaggatgcgc gccgcctcct ccacccgtgc
    21001 cttgatctcc ggcttcggat agcgcttcag ccgcagcccg aacgac
//
LOCUS       DASVDU010000094        10055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1254815, whole genome shotgun sequence.
ACCESSION   DASVDU010000094 DASVDU010000000
VERSION     DASVDU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254815"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF226_06160"
     CDS             <1..332
                     /locus_tag="VF226_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003586164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7073608.1"
                     /translation="VPVCADESAHTTDGLLALRDRYDAVNIKLDKTGGLTEALRMARE
                     ARRLGFRIMAGCMVATSLAMAPAVLVAQLADWADLDGPLLLARDREWGLEIRDGMISP
                     PDPRLWG"
     gene            complement(352..1533)
                     /locus_tag="VF226_06165"
     CDS             complement(352..1533)
                     /locus_tag="VF226_06165"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7073609.1"
                     /translation="MSLSTTSPRGFSRRLSFFYAALFVVYGIQLAYFPVWLDWRGLSA
                     AEIGMVSAAPLFLRLAIGPTAAFLADRSGDRRKAVIIAASCGCAAVLALSQSHLLLAV
                     ALFTAIFVVGCQTTGPMAEAIALSGVRELGVDYGRMRLWGSLSFIAATFTGGVLVERF
                     AAPSVIWMLAASTSLLVLTSWMLPSQKPAGGAAAGAGSRLTLADVTKVAGSRRFLLFV
                     FAAGVVQASHALFYSFGVLHWQTQGISTTTIGVLWSVGVIAEVILFAVAGRYLKRIGP
                     AGFIIAGAAAAVVRWGAMALDPAIGPLFALQVLHGLTFGATHLGAMHYIHQTVPQEQA
                     GTAQALFSAATGGAAMGAATLLAGLLYGPFGGLGYLAMMAMGGAGLIAAVALRMQPGG
                     S"
     gene            1647..2354
                     /locus_tag="VF226_06170"
     CDS             1647..2354
                     /locus_tag="VF226_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073610.1"
                     /translation="MQQQQSYAQMSMDVEQEYRAIESALLNNPRGRWFLAEHGRRARR
                     LDSAMLEMAIERLQNSIRQPPALLGQLQNEIEELKRFIIDTRAEMLARPPKVDAEAPS
                     DAPADGILRAAEDLHELAWTLQANDINAEACEKIARQASAIYALSVRQALESERTQKL
                     AVALDAACARLNALLETIDHESQFDDAPPPPSDEVESMTALLTEQIMPPTDELDGVSN
                     SDDGRKPADLGDLIDSD"
     gene            complement(2420..3400)
                     /locus_tag="VF226_06175"
     CDS             complement(2420..3400)
                     /locus_tag="VF226_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX7073611.1"
                     /translation="MEPPLPTYEDVLAAAERIGGLAVRTPLIEHPVLNDRAGGRVLLK
                     LETLQRVGSFKFRGAFNAISKLDRTKYPGGVVACSSGNHAQGVAAAAMMCSMDALIVM
                     PKDAPEMKVARTRRYGAEVVGYDRETEDRDAMARALCAERNAAFIPPFDHPDVIAGQG
                     TVGLELMQQADAMNAKPDLVLAPCSGGGLVTGVALAVQHFNPAAEVYSAEPEGFDDLA
                     RSLASGSRERNERMSGSICDALLAPTPGEITFALAKQRLAGGAAVSEDEVRAAVRFAF
                     EELKLVVEPGGAAALAAILAGKVPTVGRTVACVLSGGNVDPALFSEIIAA"
     gene            complement(3834..4667)
                     /gene="thiD"
                     /locus_tag="VF226_06180"
     CDS             complement(3834..4667)
                     /gene="thiD"
                     /locus_tag="VF226_06180"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7073612.1"
                     /translation="MAETLPSLPIAMTIAGSDSSGGAGIQADLKTFTALGVYGASVLT
                     ALTAQNTRGVDGVMAVPPAFVTAQLDSVLSDLTVGATKTGMLANAGIILAVADSLQSC
                     PEIPLVCDPVMIATSGDVLLEPDAIDALRERLIPRAALITPNIPEAARLLGCEEADDV
                     DGMARQAEALRSLGCGAVLLKGGHGSGKMATDLLVHSGGMTRLEAPRIPTSNTHGTGC
                     TLAAAITALLAAGENLEQAVRRAKTYVWRALEAGAGLRIGHGSGPVDHLFAIRRQPMP
                     A"
     gene            complement(4752..5555)
                     /locus_tag="VF226_06185"
     CDS             complement(4752..5555)
                     /locus_tag="VF226_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7073613.1"
                     /translation="MFIDAHIYLGVALAGLVSFLSPCVLPLVPPYLGYLGGTTINMAA
                     SHETKIDPAVWQRVVLGSVVFVLGFTTVFVGLGAGASAFGQVIQTYRNELSIVAGAVI
                     IIFGLHFLGVLRVPLLYKEARYHGEVQATGLFGAYVIGLAFAFGWTPCVGPILATVLA
                     LAANEASLGKGVQLLFVYSLGLGLPFILAAVAIRPFLSFLRRFRRHLGAMEKAMGLLL
                     VVTGIMFLSGSMNWLGQWMIETFPGLATIEEQFTPKNLQIDIMKQGLGN"
     gene            complement(5672..6793)
                     /gene="recA"
                     /locus_tag="VF226_06190"
     CDS             complement(5672..6793)
                     /gene="recA"
                     /locus_tag="VF226_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7073614.1"
                     /translation="MQTPHLRVVEGDTMDKQKALEAALSQIEKNFGKGSIMRLGAHDR
                     SIDVEAIPTGSLGLDIALGIGGLPRGRIIEIYGPESSGKTTLALHVIAEAQKRGGICG
                     FVDAEHALDPVYARKLGVKVDDLLISQPDTGEQALEITDTLVRSGAMDVLVVDSVAAL
                     TPKAELEGEMGDALPGMQARLMSQALRKLTASISKSRCMVIFINQIRMKIGIMFGNPE
                     TTTGGNALKFYASVRLDIRRIGQIKDRDEVVGNQTRVKVVKNKVAPPFKQVEFDIMYG
                     EGVSRTGEIIDLGVKGGIIEKSGAWFSYDGQRIGQGRENTKAFLKENPQIANKIEQAI
                     RQNAGLIVDRLLAQPEENEAIEAVDGPEEPTPAVGRGRR"
     gene            complement(7039..9525)
                     /locus_tag="VF226_06195"
     CDS             complement(7039..9525)
                     /locus_tag="VF226_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7073615.1"
                     /translation="MSARSGGGMVALVAVALAGSFGALAILSADFSEPLLLTAMAFLA
                     MLGVFFLFGLAAGHIRIAEPSADNRILSALAASFEDGLCVTTLEGRRIYANRAFDDLV
                     GPVEYDHVSALESLFAGEPQAAEALFRLARAAGRGENRTEEFRLRTIAGGQRQSRWLR
                     LGVTRIGMGQGDENEPLVLWRLVDITDERRREVETVRALEATLGYYESMPLGLMVAGE
                     DASIEHLNGTLATWLGLSAVSLARGLKLTDIVSGDGALLLRALARDADAGAARRIDLD
                     LVCEDGRSWPATLMVVPRAGDAGFVAAVLERAAEEDAAGLDRTAEERFARFFQSAPFG
                     IATVSADGKIASANTAFARLMLNGRAGRGDMALDVLTRSVDSERRAEVAAALDDALAG
                     KANIQPLEITVGDSREFTRRVFFSALTRGSAAESAVLYVIDATELKALEFQFAQSQKM
                     EAVGKLAGGVAHDFNNVLTAIIGFSDLLLQTHRPGDPAYQNIINIKSSANRAAELVSQ
                     LLAFSRRQTLQPEVLQLNEIVTDTSVILNRLLGEKIELKILPGRDLWYVKADRSQLQS
                     VIVNLAVNARDAMPDGGRLTIRSRNIGERESQKLEPQSLPIGEYVMIEVEDTGHGMTP
                     EVQAKIFEPFFTTKSVGKGTGLGLATVYGIVKQTGGYVFVHSAPGKGTTFRVYLPRHF
                     LDAEGEAGLADEKAAKKERDSDLTGAGRVLLVEDEDAVRGFAVEALKRQGYEVLEATS
                     GAEAIELVSKADRPIDIVVSDVIMPEMDGPSMYKVLRQKNPGLKIVFMSGYPDDAFKN
                     ALEADETFTFLQKPFSLAQLAAKVKAELGR"
BASE COUNT         1895 a         3337 c         3133 g         1690 t
ORIGIN      
        1 ccgttccggt ctgtgccgac gagtcggccc acacgacaga cggtcttctc gcgctccggg
       61 accggtacga cgcggtcaac atcaagctcg acaagaccgg cgggctgacg gaggcgctgc
      121 gcatggcccg ggaagcccgg cgcctcgggt tccgcatcat ggccggctgc atggttgcga
      181 cctcgctggc gatggcgccc gccgtacttg tggcccagct tgccgactgg gccgacctcg
      241 acggcccact gctgctcgcc cgcgatcgcg agtggggcct cgagatccgc gacggaatga
      301 tatccccgcc ggatccgcgg ctctggggct gagccggacg tacggcgcga gctatgaccc
      361 gcccggctgc attctgagcg caacggctgc gatgagcccg gcaccgccca tggccatcat
      421 ggcaagataa ccgagccccc cgaacggccc atagagcagg ccggccagca acgtcgccgc
      481 ccccatggcg gcgccgcccg tcgccgcaga aaagagcgcc tgcgccgtcc cagcctgctc
      541 ctgcggcacg gtctggtgga tgtagtgcat cgccccgaga tgggtggctc cgaacgtcag
      601 cccgtgcagc acctgcaaag caaacagcgg gccgattgcc ggatcgagcg ccatggcgcc
      661 ccagcgaacc accgctgccg cagcaccagc aatgatgaaa cccgctgggc cgatacgctt
      721 caggtagcgc ccagcgacgg caaacagaat gacctcggcg atcacgccca cagaccagag
      781 cacgccgatc gtcgtcgtcg agatgccctg cgtctgccag tgcaacacgc cgaaagaata
      841 gaacagggca tggctcgcct gcaccacacc cgctgcgaac acaaacagga gaaaccggcg
      901 cgatccggca accttggtca catctgcgag cgtgagccgg ctgcctgctc cggccgcagc
      961 gcctccggcg ggcttttgcg agggcagcat ccaggacgtc aggacgagca agctcgtgct
     1021 tgccgccagc atccagatca cggatggcgc agcaaaccgc tcgacgagga cgccgcccgt
     1081 gaacgtcgcg gcgatgaagc tcagcgatcc ccaaagccgc atgcgaccgt agtcgacgcc
     1141 gagctcgcgg acccccgaca gcgcgatcgc ctcggccatc ggcccggtgg tctggcaccc
     1201 gacaacgaaa attgccgtga agagcgcgac cgccaggagg agatgcgact ggctcaacgc
     1261 gagcacggcg gcacaaccgc agctcgccgc gatgataacg gcctttcgcc ggtccccgga
     1321 ccggtcggcg aggaacgccg ccgtgggccc gatggccaat cgcagaaaca atggcgccgc
     1381 cgacaccatc ccgatttcag ccgccgagag tccgcgccag tcgagccaga cgggaaagta
     1441 agcgagctgg ataccgtaga cgacgaacag cgccgcgtag aaaaacgaca ggcgccggct
     1501 gaacccgcgt ggcgaggtcg tggacaggct catgagcgcc agggaggcaa aaaggccttt
     1561 ggacgaatca gccagaccgg attcctcacc atcgaatcgg cgtatctctt ggaaaacagc
     1621 atcttgcgag tgatcggatt tgccccatgc agcaacagca gtcgtatgcc cagatgtcga
     1681 tggacgtcga gcaggaatat cgcgccatcg agtcggctct gctcaacaac ccgcgcggcc
     1741 gctggtttct ggccgagcat ggccggcggg cgcggcggct cgacagcgct atgctcgaaa
     1801 tggcgatcga gcgcctgcag aattcgatcc ggcagccgcc tgcgctcctg ggccagctcc
     1861 agaacgagat cgaggagctg aagcggttca tcatagacac acgcgccgag atgctcgcgc
     1921 ggccgccgaa agtcgacgcc gaggcgccgt ctgacgcgcc ggccgacggc attctgcggg
     1981 ccgccgagga tctgcacgag ctcgcctgga cgcttcaagc caacgacatc aatgccgaag
     2041 cttgcgagaa gattgcccgg caggcttcgg cgatctacgc actgagcgtc cggcaggcgc
     2101 tcgagtcgga gcgaacgcag aagctcgccg ttgcgctcga tgccgcctgc gcgcgcctca
     2161 atgcgctcct tgagacgatc gaccacgagt cacagttcga cgacgctcct ccgccgccat
     2221 cggacgaggt cgaatccatg acggctctgc tgaccgagca gatcatgccg cccacagacg
     2281 agttggacgg ggtgtcgaac tcggacgacg gcagaaagcc tgccgacctt ggagacctca
     2341 tcgactcgga ttgagcgaca acgtacgccg gtctcaaatc aagagatccg agcttttgct
     2401 cggatctttt ttgtccggat caggctgcga tgatttcgga gaacaatgcg ggatcgacat
     2461 ttccgcccga gagcacgcag gcaaccgtgc ggccgacggt tggcaccttg cccgcaagga
     2521 tcgccgcaag ggccgccgcg ccacccggct cgacaaccag cttcagctcc tcgaatgcga
     2581 atcgtacggc tgcccgcacc tcgtcctcgc tgacggcagc tccgcccgcg agcctctgct
     2641 tcgccagcgc gaacgtgatc tcgcctgggg tcggagcgag caatgcatcg caaatcgagc
     2701 ctgacatcct ctcgttccgc tcccggctgc cgctcgccag cgagcgcgcg aggtcgtcga
     2761 agccctccgg ctctgcgctg tagacctccg cagccggatt gaaatgctgg acagcaagcg
     2821 ccacaccggt gacgaggcca ccgccggaac atggcgcaag gaccagatca ggcttggcgt
     2881 tcatggcgtc cgcctgctgc ataagctcga ggccgacggt gccctggccg gcgatgacgt
     2941 cgggatgatc gaacggcgga atgaacgcag cattgcgctc ggcgcagagc gcacgcgcca
     3001 tggcatcgcg gtcctcggtc tcgcggtcgt agcccaccac ctccgcgccg tagcgcctgg
     3061 tgcgcgccac cttcatttcc ggcgcgtcct tgggcatgac gatcagcgcg tccatgctgc
     3121 acatcatagc cgccgcggcg acaccctggg cgtgattgcc cgatgagcaa gcgacgaccc
     3181 cgccggggta cttggtgcgg tcgagcttcg aaatcgcgtt gaaggcgcca cgaaatttga
     3241 aggagccgac gcgctgcaaa gtctcgagct tgaggagcac gcggccgccg gcccggtcat
     3301 tcaggaccgg gtgctcgatg agcggggtcc gaaccgcgag ccctccgatg cgctcggcgg
     3361 cggcgaggac atcttcatat gtcggcaggg gaggctccat ggtgccaagc tccaagtaag
     3421 agaaactgtg gtctgatgcg gcgcattcag cagaaggccg gcgtcgccct gagacgccgg
     3481 cccatcgaac accggattag cccatttcgg cggcatttcg ccacctgcat tttggctttg
     3541 cgcggatgcc cgctggggta tcgtcgatac atccgctcgt gaaaaagaga aaccggcccg
     3601 gatagaccgg gccggttctt cgatagagcc agaccatgtc cggctctccc cccgagctca
     3661 catgcgccgg gtctccccca gtccggcgct ggtcatgccc ccgtgagcga atttgtccag
     3721 ggaacactgg catttatcgg cgcggccgac aactgggtga gaactgatta ccggggaacc
     3781 ggtgcggaaa agcaacaggt ggtcaggtct ccggaaccaa acgtctcgat ctctcacgcc
     3841 ggcatgggct ggcgtctgat cgcaaacagg tgatcgaccg gcccggagcc gtggccgatg
     3901 cgaagtccgg cacccgcttc gagcgcgcgc cacacgtacg tcttggcacg gcgcacagcc
     3961 tgctccagat tttcccctgc cgccagaagc gccgtgatgg cagccgcgag cgtgcagcct
     4021 gtcccgtgcg tgttgcttgt cggaatccgg ggtgcttcga ggcgagtcat gccacccgag
     4081 tgcaccagca gatcggtggc catttttccg ctgccatggc cgcccttgag cagcaccgcg
     4141 ccgcagccca gcgaacgcag cgcctctgcc tgccgtgcca tcccgtcgac gtcgtccgct
     4201 tcctcgcaac cgagcaaccg ggcagcctcg ggaatgttcg gcgtgatgag ggctgcgcgg
     4261 ggaatcaacc gctctctcaa agcatcgatt gcgtccggct cgagcagcac gtcgccactc
     4321 gtcgcaatca tcacggggtc gcaaacgagc gggatctcgg gacaagactg cagagaatca
     4381 gcaacggcca gaatgatgcc ggcgttggcc agcatgcctg tcttggttgc gccgaccgtg
     4441 aggtccgaga gtacggagtc gagctgtgct gtcacgaagg ccggcgggac agccatcacg
     4501 ccatcaacac cgcgtgtatt ctgcgccgtc agcgccgtca aaaccgaagc gccgtacacg
     4561 cccaatgccg tgaatgtctt gaggtccgcc tggatgcccg cgcccccgga gctatccgag
     4621 ccggcgatcg tcatcgcaat cggaagcgaa ggcagtgtct cagccattca cgtcctcaca
     4681 ttcttaggca tgattgtcgc tgaacgatgc gccgggacag agtgtcggtc tcccgtcatc
     4741 cgcacccgac ctcagttccc gagcccctgc ttcatgatgt cgatctgcag gttcttgggc
     4801 gtgaactgct cctcgatcgt cgccaacccc gggaaggtct cgatcatcca ttggccgagc
     4861 caattcatcg agccgctgag gaacatgatg ccggtcacga ccaggaggag acccatggcc
     4921 ttctccatcg cccccaaatg ccggcgaaag cgccggagga acgagaggaa cggccgaatg
     4981 gcaacggcgg cgagaatgaa tgggaggccg agcccgagag aatagacaaa cagaagctgc
     5041 acacccttgc caaggctggc ctcgttggcg gccaaggcga gcaccgtagc cagaatcggg
     5101 ccaacgcagg gcgtccaacc gaacgcgaaa gcgagcccga tcacgtaggc gccgaagaga
     5161 cccgtcgcct gcacctcgcc gtgataacgc gcctccttat agagcaatgg gacgcgtagc
     5221 acgccgagga agtgcaaacc gaagatgatg atcaccgcac cggcgacgat cgacagctcg
     5281 ttccggtagg tctggatgac ttgaccgaag gccgaggcac cggcaccgag gccaacgaat
     5341 accgtcgtga agccgaggac gaacacgacc gatccgagca ccacgcgctg ccagacggcg
     5401 gggtcgatct tcgtctcgtg cgacgcggcc atgttgatcg tcgtaccacc gaggtagccg
     5461 agatacggcg gcacgagggg cagcacgcac gggctcagga aactcacgag ccctgccaaa
     5521 gccacaccga gatagatatg cgcgtcgatg aacacgtgaa ccaaatgcct ttagcttcgc
     5581 cggcagcctg ccttagcttg cccaccctgt cacgcgtgac atgccggcgc aggtaaacaa
     5641 tgccggtgcc cggactttcc gagggcgttc atcatctccg gccgcgaccg actgcggggg
     5701 tcggctcctc cggcccatcg accgcttcga tcgcctcgtt ctcctcgggt tgggccagca
     5761 gccgatcgac gatcagaccg gcattctggc gaatcgcctg ctcgatcttg ttcgcaatct
     5821 gcgggttttc cttgaggaat gccttggtgt tctcgcggcc ctgcccgata cgttgtccat
     5881 cataggagaa ccacgcaccg gacttctcga taattccccc cttgaccccg aggtcgatga
     5941 tctcgccggt ccgggagacg ccctctccgt acatgatatc gaactcgacc tgcttgaagg
     6001 gcggcgccac cttgttcttg acgaccttca cgcgggtttg attgccgacg acctcgtcgc
     6061 gatccttgat ctgaccgata cggcggatgt cgagccggac ggaggcatag aacttgagcg
     6121 cattgccgcc cgtggtcgtt tccgggttgc cgaacatgat gccgatcttc atccggatct
     6181 gattgatgaa gatcaccatg cagcgcgact tggagatcga agccgtgagc ttgcgcagcg
     6241 cctgactcat gagccgggcc tgcatgccgg gaagcgcgtc gcccatctcg ccctcgagct
     6301 ctgccttggg cgtcaaggcc gcaaccgaat ccaccaccag gacgtccatt gccccggagc
     6361 ggaccagcgt gtccgtgatc tcgagcgcct gctcgcccgt atccggctgg gagatgagca
     6421 agtcgtcgac cttcaccccg agcttacggg catagaccgg gtcaagcgca tgctcggcat
     6481 cgacgaagcc gcagatgccg ccgcgcttct gcgcctccgc gatgacatgc agggccagcg
     6541 tcgtcttgcc ggatgattcc ggtccgtaga tctcgatgat gcggccgcgc ggcagaccac
     6601 cgatgccgag ggcgatatcg aggccgagcg agccggttgg aatggcttcc acgtcgatgg
     6661 agcggtcatg cgctccgagc cgcatgatgg agcctttgcc gaaattcttc tcgatctgcg
     6721 acagggcggc ttcgagcgcc ttctgcttgt ccattgtgtc tccctcgacg actcggagat
     6781 gcggtgtttg catcggcgtc accctatggc agcggtttca gatcggcaat tggttaaccc
     6841 aatgtacgcg atttgttctc atctgtcgag ggtgaactga cggttgattt cactcagatt
     6901 ttcctatcgg atacatgaaa tgttctcact gcgcttcggc acaagagaga aaccactccg
     6961 ggtgactgcg gcggttaggc cgattcggct aagccgacca gaaggaaaac cctggtccgc
     7021 gcgaaccacc gagccggcct agcgcccaag ctccgccttc accttggccg caagctgcgc
     7081 cagcgagaaa ggcttttgca ggaatgtgaa cgtctcatcg gcttcgagcg cattcttgaa
     7141 agcatcatca ggatagccgg acatgaacac gattttcagt ccggggtttt tctgccgcag
     7201 caccttgtac atgctgggcc catccatttc aggcatgatg acgtcgctca cgacgatgtc
     7261 gatgggccgg tctgccttgc tcaccaactc gatggcctcg gcgccgctcg tcgcctccag
     7321 cacctcgtag ccctgccgtt tcagcgcctc gaccgcgaag ccacggacgg catcctcatc
     7381 ctcgaccagg aggacccggc cagcgccggt cagatcggaa tcccgctcct tctttgccgc
     7441 cttttcgtcg gcgagacccg cctcaccctc ggcatcaagg aaatgccgcg gcaggtaaac
     7501 ccggaaggtg gtccccttgc cgggcgcact gtgcacgaac acgtagccgc cggtctgctt
     7561 gacgatgccg tacaccgtcg caaggccgag accggtcccc tttccgacgc tcttggtcgt
     7621 gaagaacggc tcgaagatct tggcctgcac ttcgggtgtc atgccgtggc cagtatcctc
     7681 gacctcgatc atcacgtact cgccgatcgg caggctctgc ggctccagct tctggctttc
     7741 gcgctcgccg atgttgcggg agcggatggt caagcgcccg ccgtccggca tggcatcgcg
     7801 tgcgttgacc gccaaattga cgatcaccga ctgaagctgt gagcggtccg ccttgacgta
     7861 ccacaggtcc cgccccggca ggatcttcag ctcgatcttc tcgcccaaaa gccggttcag
     7921 gatcaccgag gtgtcggtga cgatctcgtt gagctgcagc acctccggct gcagggtctg
     7981 gcgacgggag aaagcgagca gctgactgac gagctcggcg gcgcggttgg cgctcgactt
     8041 gatgttgatg atgttctggt aggcgggatc gcctggccga tgggtctgca gcagcaggtc
     8101 ggagaagccg atgatggccg tcagcacgtt gttgaagtcg tgcgcgacgc cgccggcgag
     8161 cttgccgacc gcctccatct tctggctctg cgcgaactgg aactcgagcg ccttgagctc
     8221 ggtggcgtcg atcacgtaga gcacggccga ttcggccgcg gagcctctcg tgagggcgct
     8281 gaagaagact cttctggtga attcccgcga gtcgccgacg gtgatctcca gcggctgaat
     8341 gttggccttg ccagcaagcg cgtcgtcgag ggctgctgcg acctccgccc ggcgctccga
     8401 atcgacgctt cgggtcagaa cgtcgagggc catgtcgccg cggccagcgc ggccattgag
     8461 catcagccgg gcgaaggccg tgttggcgct tgcgatcttt ccatccgccg atacggtggc
     8521 gatgccgaag ggggcggact ggaagaacct ggcgaaacgc tcttctgccg tgcggtccaa
     8581 gccggctgca tcctcctcgg cggcccgctc cagaaccgcg gccacgaacc ctgcatcgcc
     8641 ggcgcgcggc acgaccatca gcgtggcagg ccagctgcgg ccgtcctcgc acacaaggtc
     8701 gaggtcgatc cggcgcgcag caccggcatc ggcgtcgcgc gccagggctc tcagcaggag
     8761 cgcgccgtca ccggagacga tgtccgtcag cttcaggccc cgggccagcg agaccgcgct
     8821 caacccgagc caagtcgcca acgtgccgtt caggtgctca atgctcgcat cctcgccagc
     8881 caccatgagg ccgagcggca tggactcgta atagccgagt gtcgcttcga gcgcgcggac
     8941 cgtctcgacc tcgcgccggc gctcgtccgt gatatcgacg agacgccaca agaccagcgg
     9001 ttcgttctcg tccccctgcc ccatgccgat ccgcgtgacc ccgaggcgaa gccagcggga
     9061 ctgccgctgg ccaccggcaa tggtgcgaag acggaactcc tcggtcctgt tctcgccacg
     9121 acctgccgcg cgcgccagcc ggaacagggc ctcggccgct tgcggctcgc ctgcaaaaag
     9181 cgactcgagc gcgctgacgt gatcgtactc gacgggaccg accagatcgt cgaaggcgcg
     9241 gttggcgtag atgcggcggc cttcgagcgt cgtgacgcag aggccgtcct cgaagctggc
     9301 ggcaagggca ctcaggatcc tgttgtcggc cgacggctcc gcgatcctga tgtgaccggc
     9361 cgccaacccg aacaggaaga agacgcccag catggccaga aacgccatcg ccgtgagcag
     9421 cagaggctcg ctgaaatctg ccgaaagaat cgcgagcgcg ccgaagctcc ccgcgagcgc
     9481 aaccgcaacg agggccacca tacccccacc ggaacgtgca ctcaccaggg gaacgtcagc
     9541 cgccaaagat ccctgccgcg cctcgatttc cctcatgatc ggggaaatta gctgattctg
     9601 ggggagggca gtcttgccgg ccatcataag ctactcttac gcacaacatt tacaccggtt
     9661 cacgaagccg gacgtcagcg tggccaaatc tgcgcaatgg cgtttcggaa ccgcgctgat
     9721 ttcaagcttt cggtcatccc ggtaaagata tcgttgccgc ctgtccgacg ccgtcgtgca
     9781 tctgtcgggg catagtcatg gctcgcgggg cgagttgttg ctccattttg gttaatctgc
     9841 ctcaagaacc gcgctatacc actggctgca acggcggggg tgcgaacgga tgccgagcgg
     9901 gcgaggcggt ggccgccctc gcggcgccat gagaacgtgc acgcctccaa ccagcagatc
     9961 gagaggagcg cgggggcgcc cgcgtcgagc caaatggaaa tcctgtactg gctgcttttc
    10021 gtgattttca tcgccgccct cctggtggtg agcgg
//
LOCUS       DASVDU010000095         7908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1171385, whole genome shotgun sequence.
ACCESSION   DASVDU010000095 DASVDU010000000
VERSION     DASVDU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171385"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF226_06200"
     CDS             complement(<1..228)
                     /locus_tag="VF226_06200"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX7073616.1"
                     /translation="MSNQRNLTELHPELLAYAQSLTLDKAAAEDLVHDALVRALQSKA
                     VPRKADEVRPWAFRVLKNLFLDVRRRQKVRAG"
     gene            410..826
                     /locus_tag="VF226_06205"
     CDS             410..826
                     /locus_tag="VF226_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX7073617.1"
                     /translation="MADSPKWRDVRAPSLEDIEQLAAEAWEALPAHFREMCSDVVVRV
                     EEFATDEVLQQLGIDSPFDLMGLYHGVSLDKRSFSDLPQQPDMVFLYRRALLDYWADS
                     DETLGDIVTHVLVHEIGHHFGFSDEDMERIEAAAKF"
     gene            963..1481
                     /locus_tag="VF226_06210"
     CDS             963..1481
                     /locus_tag="VF226_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073618.1"
                     /translation="MTLRASIKFVLASIVAAAGVAVTASTAAPPTPEQYVSHVGGEAK
                     IVPVGQLVLDNRRLYCGQRPTVLDPKLDDYGAAYPGFLILNPRLLSRVSTPVKLWIHA
                     HECAHQFRGPDEETADCFAVQRGRREGWLTPEGLEEVCKFITPARSTSMHLSGSHRCE
                     AMRRCYADKRVR"
     gene            1574..2179
                     /gene="leuD"
                     /locus_tag="VF226_06215"
     CDS             1574..2179
                     /gene="leuD"
                     /locus_tag="VF226_06215"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7073619.1"
                     /translation="MQKFTTLTGVAAPLPMINIDTDMIIPKQFLKTIKRTGLGKSLFY
                     ELRYDENGNENPDFVLNKPAYRNAEILIAGDNFGCGSSREHAPWALLDFGIRCVISTS
                     FADIFYNNCFKNGILPIKVKPEELEKLLDDASRGANAKITVDLEKQEIRGPDGGVISF
                     EIDPFRKHCLLNGLDDIGLTMQKEHAIKAYEEKVAAARPWA"
     gene            complement(2233..3135)
                     /locus_tag="VF226_06220"
     CDS             complement(2233..3135)
                     /locus_tag="VF226_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix domain-containing
                     protein/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7073620.1"
                     /translation="MLQQLDFHTMHPVQDEPTRDYAIVRRAIAFLSENWDEQPDLDRL
                     ARHLGLSPAHCQKVFKRWCGLSPKEFVQAITADHARRLLADSASVLECAHEVGLSGGS
                     RLHDLFVSHEAMTPGDFKRRGAGLELAYGFHASPFGEALLMVTSRGLAGLAFVNEDEG
                     QTRADVLADMTRRWPRATYVEAPERTQPHFERIFDVSQWSPEQPVRIVMIGTDFEVRV
                     WETLLKIPMGRAVSYSDIARHIGCPTASRAVGSAVGRNPLSFVVPCHRVLRGDGSLGG
                     YHWGVTRKRALIGWETGQLLSRAG"
     gene            complement(3237..3710)
                     /locus_tag="VF226_06225"
     CDS             complement(3237..3710)
                     /locus_tag="VF226_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX7073621.1"
                     /translation="MQHDSGFHAILTPHRSLSPVGFVILMTIIGVVSFVLGMMFLMMG
                     AWPVFGFLGLDVLLIYIAFKLNYRSGRLFELVDVTRELVTVTRVHPCGRKESFDFNPF
                     WARVLLREWPDGRTELRLSAHGRELLFGQFLTDEERRDFASALKGALVDARGARI"
     gene            3770..4489
                     /gene="nth"
                     /locus_tag="VF226_06230"
     CDS             3770..4489
                     /gene="nth"
                     /locus_tag="VF226_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7073622.1"
                     /translation="MDAKEKFSSLRRSKAKAVKPRSRAKLSPDQIREIFRRFATANPE
                     PKSELEYHNPFTLLVAVVLSAQATDAGVNKATRSLFKIADTPEKMLALGEDRLREAIR
                     TIGLYRNKAKNVIALSQELVSKYGGEVPKDRESLETLPGVGRKTANVVLNVAFGQPTM
                     AVDTHVFRVANRTGLADGKTPLEVEQQLLKIVPPEYARHAHHWLILHGRYVCKAKKPE
                     CPRCLINDLCTYPAKTLPTEL"
     gene            complement(4493..6193)
                     /locus_tag="VF226_06235"
     CDS             complement(4493..6193)
                     /locus_tag="VF226_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7073623.1"
                     /translation="MARLARSLDHRKDRYDVIVVGSGYGGGVAASRLSRAGKRVAVLE
                     RGREFLTGEFPTRFPDLRNELQVRGKRMNMGRDTALYDVRIGDDMHVLVGCGLGGGSL
                     VNAGVALEPDSRVFTDEIWPGQIAQDGLLAEGFRRARQWLSPSPDPRASEHTKFKALA
                     IAGRTLDAEPIAPTVVVNFEERVNVAGVAQPACTRCGDCCGGCNVGAKNTVALTYLPD
                     AVRHGAHLFTEAMVRSLSRDPDGRWRIQVRRLDQSNGNEAEITLSAEIVILAAGTLGS
                     TEILLRSQAEGLALSPRLGERFSANGDIIAFGYGANVPVNAVGVGHPPRFEVAGSVGA
                     CVSGQIELRDAGELAQELTVQEGVLPSALAPVLPVLFMPNGRLLGAVQSLINGVYKGP
                     FARLQTFFAVSHDTSAGRLVLNGERVSVSWPGAKDEPVYQRLDAILSKLVGGVGGSYV
                     KNPLSGTLMGHQPATAHPLGGCTVARERADGVVNHKGQVFDGRPRAGPSDVHEGLYVM
                     DGSVIPRSLGVNPLLTITALAERAMLHMAEDYGLSFDTEAIPVAVPATPTTPGAFASG
                     "
     gene            complement(6305..7267)
                     /locus_tag="VF226_06240"
     CDS             complement(6305..7267)
                     /locus_tag="VF226_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7073624.1"
                     /translation="MSEPSVEPAGEERDSVPLPPAPDPVSIDLSAAEGLPKSAQRRMW
                     SASLLAAVSAHVLVLIGAAWAPSLRSVGAGGPELEAIDVDIVSSRVLESRLAGAQADA
                     AAPEVVDDAAPGAAPSQASMAAVDQMPAETKPEPQPAAPTPDLVIPETKQEEPPPEPD
                     EIALAIADKQPEKPDDQQPETEPEQNAEPQPQSAAPSQGSEASESADEGGAVARGMDL
                     VDTPGQQAAAASPGVANEYAKIVIETLARRKPRATAGVRGTVRIGFRVSHSGDVQDVR
                     VLRSSGRKVLDEAAVIAVSSTKFPAPPPALANAQLSYEVPYIFR"
     gene            complement(7408..>7908)
                     /locus_tag="VF226_06245"
     CDS             complement(7408..>7908)
                     /locus_tag="VF226_06245"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEX7073625.1"
                     /translation="ISEQTGETCNFGVLAGSEVIYLDRVEAKWPLALRFEAGSRVPAH
                     CTAIGKLLLALLPAHERMAAIASMPLTPCTSRSITDPAKLSEALERINRTRIGVDDQE
                     FIEGVVCVSVPVVTSGGQVVGAIAVSAPAARMSLQEALSFVPAMHDAAKKLSATYAID
                     TAASKS"
BASE COUNT         1447 a         2559 c         2522 g         1380 t
ORIGIN      
        1 tccggcacga accttttgcc ttcgccgcac atcgagaaac aggtttttga ggacgcgaaa
       61 cgcccagggc cgcacctcgt cggccttccg cggaaccgcc ttcgactgca acgcccgcac
      121 cagggcatca tggacgaggt cttcggccgc tgccttgtcc agggtcaagc tttgcgcata
      181 ggcaagcagt tccgggtgca actccgtcag gttgcgctgg ttcgacacgt cggcttcaac
      241 ctcccctcga cccagtcgtc gcccaatgta ccggagaccg aaatcccaac gacctccaat
      301 ctgctcctgg gagttgctct tcggcaagaa gggtcgcagg ccgttgaccg gacgcaactg
      361 gcacgccaaa tcgcttgtcg aaccgtgttt ctagaatcaa tcgctggaaa tggccgacag
      421 ccccaagtgg cgcgatgtcc gcgcgcccag ccttgaagac attgagcaac tggctgcgga
      481 agcctgggag gcgctgcccg cgcatttccg cgagatgtgc agcgacgtcg tcgtgcgggt
      541 ggaagagttc gcgacggacg aggtgcttca gcagctcggc atcgacagcc ccttcgatct
      601 catgggtctt taccacggag taagtctgga taagcgaagc ttcagtgatc tgccgcagca
      661 gccggacatg gtgttcctct atcgccgtgc cttgctcgat tactgggccg acagcgatga
      721 aacgcttggg gatatcgtca cgcacgttct cgtgcacgag atcggccatc atttcggctt
      781 ctccgatgaa gatatggaaa ggatcgaggc cgcggcgaaa ttctgatctt gagtttttcc
      841 aggcccttcg attagtcggc tacgcccggc ttgcgcgcga tctcgaggta ggcgattgtg
      901 cgctcgatcg tccgtcgtat gacgcaccaa aacacgcgga caacggatca cgtggggtgg
      961 ggatgacgtt gagagcatcg atcaagttcg tgcttgcttc gatcgttgct gcggcaggcg
     1021 ttgcggttac ggcgagcacg gctgcgccac cgacgccgga gcaatatgtt tcccacgtcg
     1081 gaggggaggc gaagatcgtt ccggtcggcc agctcgtcct cgataaccgg cgcctctatt
     1141 gcggccagcg gccgacggtg ctcgatccca agctggacga ttacggtgcg gcctatccag
     1201 gcttcctgat tctcaacccg cgtttgctgt cgcgcgtgtc gacgccggta aagctctgga
     1261 ttcatgcgca cgagtgcgcg catcagttcc gcgggccgga cgaggagacg gcagactgct
     1321 ttgccgttca gcgtggccgc cgtgagggct ggctgacacc cgaagggctc gaggaggtgt
     1381 gcaaattcat tacacccgcc cgcagcacga gcatgcatct gtccgggtcg caccgctgcg
     1441 aggccatgcg gcggtgctat gccgacaagc gcgtccgttg agccgcttga ccgcgggcac
     1501 cttctcgcgt ttatgaaccc gatcggaccg ctctggaagc tcgggggcgg ggaaaactcg
     1561 gccgagggtt aggatgcaga aattcacgac gctgaccggc gtggcagccc cactgccgat
     1621 gatcaatatc gacaccgata tgatcattcc aaaacaattt ctgaagacga tcaagcgcac
     1681 ggggctgggc aagtcgcttt tctacgagct gcgctatgac gagaacggga acgagaaccc
     1741 cgatttcgtc ctgaacaagc ccgcctaccg caacgccgag atcctgatcg cgggcgacaa
     1801 cttcggctgc ggttccagtc gcgagcacgc accctgggcg ctgctggact tcggcatccg
     1861 ctgcgtcatt tcgacgagct tcgccgacat cttctataac aactgcttca agaacggcat
     1921 tctgcccatc aaggtgaagc cggaggagct ggagaagctg ctcgacgacg ccagccgagg
     1981 ggccaacgcc aagatcaccg tggacctgga gaagcaggag atccgtgggc ctgacggcgg
     2041 tgtcatctcc ttcgagatcg atcctttccg caagcactgc ctgctcaacg gcctcgacga
     2101 catcggcctg acgatgcaga aggagcatgc gatcaaggcc tacgaggaaa aggtggccgc
     2161 cgccaggccg tgggcctgat cgcgcccaga acagccgctg atctcactcc ttcattgggc
     2221 gcggcagggt gatcaccctg cccggctcag cagctggccg gtctcccaac cgattaatgc
     2281 gcgcttgcgc gtcacgcccc agtggtagcc gccgagactg ccgtcgcccc gcagaacacg
     2341 gtggcagggc acgacgaaag acagcgggtt gcgccctacg gccgagccga cggctcttga
     2401 agccgtcggg cagccgatgt ggcgcgcgat atcggaatag ctgacagcac gtcccatcgg
     2461 gatcttgagc agcgtctccc acacgcgcac ctcgaaatcc gtgccgatca tcacgatgcg
     2521 aaccggctgc tccggggacc attgcgagac atcgaagatc cgctcgaagt gcggttgcgt
     2581 ccgctccggc gcctcgacgt aggtcgcccg cggccatcgc cgtgtcatat cggcgagaac
     2641 atcggcacgt gtctgcccct cgtcctcgtt gacgaaggca agtcccgcca ggccgcggct
     2701 cgtgaccatc agcagcgctt cgccgaacgg cgacgcgtgg aacccgtagg cgagctccag
     2761 cccggctccg cgccgcttga agtcgcctgg cgtcatggcc tcgtggctga cgaacaggtc
     2821 gtgcaggcgg cttccgcccg acagcccaac ctcgtgggcg cattcgagca cgctggccga
     2881 gtcggccaga agcctgcggg cgtgatccgc cgtgatggcc tgcacgaact ccttcgggct
     2941 tagcccgcac cagcgcttga agaccttctg gcaatgggcc gggctcaggc ccagatggcg
     3001 ggcgagacgg tcgagatcgg gctgttcgtc ccagttttcg gacagaaacg cgatggcgcg
     3061 ccggacgatc gcataatcac gcgtgggctc gtcctggacg gggtgcatcg tgtggaaatc
     3121 gagctgctgc aacatggccc gcctgtgcat ctgaagagtg tatccagact ggatagttaa
     3181 ggcccctgcc gcgcgacggc gacccgattc ttgccgtcac aaccgccgtc gcccctttaa
     3241 atgcgcgcgc cgcgtgcgtc gaccagtgcg cccttgaggg cagaggcaaa atctcggcgc
     3301 tcctcgtccg tcagaaattg cccgaacagc agctcacggc catgcgccga aagccgcagc
     3361 tccgtgcgcc catccggcca ttcgcggagg agaacgcgcg cccagaacgg gttgaagtcg
     3421 aagctctcct ttcggccgca cgggtggacg cgggtgacgg tcaccagctc gcgtgtgacg
     3481 tcgaccagct cgaacagccg acctgaccgg tagttgagct tgaaggcgat gtagatcagc
     3541 aggacgtcga gaccgaggaa cccgaagacg ggccaggcgc ccatcatcaa aaacatcatc
     3601 ccgagcacga agctcaccac gccgatgatc gtcatcagaa tgacgaatcc gacggggctg
     3661 agcgaccggt gcggcgtcag gatggcgtgg aaaccggaat cgtgttgcat tcgcgggtca
     3721 ttctcggcca tgagacttgc caattctagc gcatcttcaa cggaaaacca tggacgccaa
     3781 ggagaagttc tcgagcttga ggcgatcgaa ggccaaggcg gttaagccgc ggtcgcgggc
     3841 gaagctctcg cccgatcaga ttcgcgagat ctttcgccgg tttgcgacgg ccaatcccga
     3901 gccgaagagc gagctcgagt accacaatcc gttcacgctg ctcgttgccg tcgtgctttc
     3961 ggcgcaggcg acggatgccg gggtgaacaa ggcgacgcgg tcgctgttca agatcgctga
     4021 tacacccgaa aaaatgctcg cacttggcga ggatcgcttg cgcgaggcga tcaggacgat
     4081 cggcctctac cgaaacaagg cgaagaacgt catcgcactt tcgcaggagc tggtgtccaa
     4141 gtacggcggt gaggttccca aggatcgcga gtccctcgag accctgccgg gcgtcggccg
     4201 gaagacggcc aacgtggtgc tgaacgtcgc cttcggccaa ccgaccatgg ccgtcgacac
     4261 gcacgtcttt cgcgtcgcca atcggaccgg gctggccgac gggaagacgc ctttggaagt
     4321 ggagcagcag ctgctgaaga tcgtaccgcc cgaatacgcg aggcacgccc atcattggct
     4381 gatcctgcac ggccgctatg tctgcaaggc gaagaagcca gagtgtccgc gctgtctcat
     4441 caacgacctc tgcacctatc cagccaagac ccttcccacc gagctttaat gctcatccgg
     4501 acgcaaaagc gcccggggtt gtaggcgttg ccggcaccgc cactggaatg gcctctgtat
     4561 cgaacgaaag gccgtaatcc tcggccatgt gcagcatcgc gcgctcggcg agcgcggtga
     4621 tcgtcagcag cggattgacg ccaagcgagc gtgggatcac cgagccgtcc atgacgtaaa
     4681 ggccctcgtg cacgtcgctg ggcccggccc tggggcggcc atcgaagacc tgtcccttgt
     4741 ggttgaccac gccgtccgcg cgctcgcggg ccacggtgca cccgccgagc ggatgtgccg
     4801 ttgccggctg atgacccatg agggtgcccg acagcgggtt cttcacatag ctgccgccga
     4861 ctccgccgac cagcttcgag aggatcgcat cgagccgctg ataaaccggc tcgtccttgg
     4921 cgccgggcca tgagaccgag acccgctcgc cgttgaggac caggcgtccg gcggaggtat
     4981 cgtgagagac ggcgaagaag gtctgaagac gagcgaatgg tcctttgtag acgccgttga
     5041 tgaggctctg cacggctccg aggagccgcc cgttcggcat gaacaggacc ggcaagacag
     5101 gggccaatgc ggacggcagg acgccttcct gcaccgtcag ctcctgtgcc agctcgccgg
     5161 catcgcgcag ctcgatctgc cccgagacgc atgcgccgac gctgccggcg acctcgaacc
     5221 gcgggggatg accgaccccg acggcgttga caggtacatt cgcaccatag ccgaaggcga
     5281 tgatgtcgcc attcgcggag aagcgttcgc ccagccgtgg cgacagcgcg aggccttccg
     5341 cttgcgaccg caagagaatt tccgtcgatc ccaacgtgcc ggctgcgagg atgacgattt
     5401 ctgccgacag ggtgatctcg gcttcattgc cgttcgactg gtccaggcgc cggacctgga
     5461 tgcgccagcg tccatcggga tcgcgagaga gcgaccggac catagcctcc gtgaacagat
     5521 gtgcgccatg gcgaaccgca tcaggcagat aggtcaaggc gacggtattc ttcgcgccga
     5581 cattgcatcc cccgcagcag tcgccgcagc gtgtgcaggc cggctgcgcg acgccggcca
     5641 cgttcactct ttcctcgaag ttgacgacca cggtcggcgc gatcggttcg gcatccagcg
     5701 tccgcccggc gatcgccagc gctttgaact tggtgtgctc cgatgccctc ggatcaggcg
     5761 atgggctgag ccattgccgg gcacggcgga agccttcggc gagcaacccg tcctgcgcaa
     5821 tctgaccggg ccagatctcg tcggtgaaga cccgtgaatc gggctccagc gccaccccgg
     5881 cgttgaccag tgatccacct ccgagcccac agccgacgag cacgtgcatg tcgtcgccga
     5941 tccgcacgtc atagagcgcc gtgtcgcggc ccatgttcat ccgcttgccc cgcacctgca
     6001 actcattgcg caagtcagga aagcgggtgg ggaactcgcc cgtcaggaac tcgcgccccc
     6061 gctccagcac ggcaacgcgc ttgcctgcgc gtgacaggcg tgaggccgca acgccaccgc
     6121 cgtaacccga accgaccacg atgacatcgt accggtcctt tcggtgatcg agactgcgag
     6181 cgaggcgcgc cattggttct ccgagggtga aagaggtggt cggcggtcgt cggatatgct
     6241 cgataaattg gacgatgctc ggatgagagt cgaccagtgc tcgaaaagag gtgaatgatg
     6301 gcagtcaccg gaagatgtat ggcacctcgt aactgagctg cgcgttggcg agagccggcg
     6361 gcggtgccgg aaatttggtg ctgctgacgg cgataacggc cgcctcgtcg agcaccttcc
     6421 gtccgctcga tcgcaggacc cgcacgtcct gcacatcacc ggaatggctc accctgaaac
     6481 cgatgcgcac cgtgccgcgt acgccggcgg tcgctcgcgg tttccggcgc gccagcgtct
     6541 cgatgacgat cttggcgtac tcgttggcaa cgccgggact ggctgcggcg gcctgctggc
     6601 ctggcgtgtc gaccagatcc attccccggg cgaccgcgcc gccctcatcg gccgattcgc
     6661 tcgcctccga tccttgcgag ggagctgcgg attgaggctg tggctcagca ttttgctcag
     6721 gctcggtctc gggctgctgg tcgtcaggct tttcgggctg cttgtcagcg atcgcgagtg
     6781 cgatttcgtc gggctcgggc ggcggctcct cttgctttgt ctccggaatg accagatcgg
     6841 gcgtgggagc cgccggctgc ggctcgggtt ttgtctcggc cggcatctga tcgacggcgg
     6901 ccatgctggc ctgcgatggc gccgcgccag gggcggcatc gtcgacgacc tccggggccg
     6961 cggcatcggc ctgcgcgccg gccagccggc tttccagcac tcttgaagag acgatgtcga
     7021 cgtcgattgc ttccagctcc ggcccgcccg caccgacgga gcgcaggctc ggagcccagg
     7081 cggcgccgat caggaccagc acgtgcgcgg agacggccgc cagcaggctc gccgaccaca
     7141 tcctgcgctg cgcagatttc ggcaggcctt cagcggccga caggtcgatc gagacagggt
     7201 cgggtgctgg cggcagcggc accgaatccc gctcttcacc agccggttcg acgctcggct
     7261 cgctcatccc cggttccgga acaatcctgc gtgatgcgcc tgccgcaacc tttacactag
     7321 cggcttgcga ctgtcgtgtg gcgtggccac cgcacccacc acatagcagg aaaggttatc
     7381 tcgggtcaga gcgcgtcctg cgcgccctta gctcttgctc gccgccgtat cgatggcgta
     7441 ggtggcgctg agcttcttgg cggcatcatg catggccgga acgaatgaga gcgcctcctg
     7501 cagggacatc cgcgcggccg gggcggaaac ggcaatggcg ccaacgacct ggccgccgct
     7561 tgtcacgacc gggacggaga cgcacaccac cccctcgatg aactcctgat cgtcgacgcc
     7621 gattcgcgtc cgattgatgc gctccagagc ctcgctgagc tttgccggat cggtgatcga
     7681 tcggctcgtg cagggcgtca gcggcatgct ggcgatggcc gccatacgct catgagcggg
     7741 gagcagcgcc agcagcagct tgccgatcgc cgtgcagtgt gcgggcacgc ggctgcccgc
     7801 ctcgaacctg agcgccagtg gccacttggc ctccacccga tcgagataga tgacctccga
     7861 gccggccagg acaccgaaat tgcaggtctc ccccgtctgt tcggagat
//
LOCUS       DASVDU010000096         4540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1061054, whole genome shotgun sequence.
ACCESSION   DASVDU010000096 DASVDU010000000
VERSION     DASVDU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061054"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VF226_06250"
     CDS             <1..478
                     /locus_tag="VF226_06250"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7073626.1"
                     /translation="PGAHIIAARAALFAWAARPGFRALMQAVLDNARALAEGLREAGC
                     QLYLGGTDTHMIVVDLRAEPGSAAVIERRLAGHGVLTNRVPLPRRPGDRGEGGLRIGT
                     TAMTLRGMGPDDFAGLARLMGPVLKTGTEASAIGANLSDRVLEMARAFPVPPSFIG"
     gene            507..1721
                     /locus_tag="VF226_06255"
     CDS             507..1721
                     /locus_tag="VF226_06255"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7073627.1"
                     /translation="MHSGANDGQGPLAGLKVIDAGTMIAGPLAATQLADFGADVIKIE
                     QPAIGDSMRHWTPEKDGKSLWWKVIGRNKRLITLSLSSPEGQALFRRLVETADIVIEN
                     YRPGTFERWGLGYEELSRINPGIVLVRVSGFGQTGPYAQRGGYGTVAEAFSGIPSFTG
                     FPDRPPTLPGFPLADSVAATFAAMSAMFAIYRRDHGEGRGQVIDVSLYEPLFRLVESQ
                     VIGYDQLGIVKQRQGNRLAEDSPRNIYETRDGRWIGVSASSQRTFERLAEAIGYPGFI
                     KDPRFVSNTKRCEHADELDELIAAWFKQNDSAEIMRLFEEMNVVAGPVLDISDIFQDP
                     HYAARQDIVEVPDEDFGTVRMQSVVPKFSETPGAVRHAGAGLGRDNRTVFIEELGLGE
                     DEFQRLQASKVI"
     gene            1730..2890
                     /locus_tag="VF226_06260"
     CDS             1730..2890
                     /locus_tag="VF226_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7073628.1"
                     /translation="MQEGYEGVYVVGVGQTAYEKRTTKTLHRILWEALAEAVHSARLS
                     WQQIDGLGVTSFALPPDNVTTLAEHFGIEARWLFQGLYGGASGIINMLHGARAIQAGD
                     AKVVACLTADVFDVASHNEMLDRFNGPLRDYLSPYAFGGANGVFALHTRLYMEEHGAT
                     REDFGRLAVAQRKNAQHNPNALFRGKTLSMEEYLNARQIADPLRLFDCVHPCAGGDCI
                     ILAHESIARDLGVPLVKILGGGEMHNYPANDVYSLRGGWEAFRDRMYGQAKCGPQDLH
                     FAQLYDDYPVMEFIQLEGMGICERGKVAEFLRETDVTVHGGFPINTGGGQLSAGQAGA
                     SGGMIGVVEGVLQLRGEAAERQVACERGVVAGYGLVAYGRGLSSSAAILAKA"
     gene            2906..3322
                     /locus_tag="VF226_06265"
     CDS             2906..3322
                     /locus_tag="VF226_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7073629.1"
                     /translation="MEKPLPRITGVDRPFWEAADSGRLMVQRCQSSECGRHILYPRVC
                     CPYCGGGDLSWEEASGRGHIRSYTFVHRPQHESFYDEAPLCFVAVALEEGPLLYGEVR
                     PRPAEAEQLLDRRVKVVFLEHAAGRKLPYFELEPIS"
     gene            complement(3495..4361)
                     /locus_tag="VF226_06270"
     CDS             complement(3495..4361)
                     /locus_tag="VF226_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7073630.1"
                     /translation="MLAPPGFVLLWSTGFVGPKFGLPYIEPMTFLSLRFALAAGALAL
                     LALCLHAPWPRTPGEIGHLAVSGALLHAGYLGGVFAALDAGAEPGVIALIAGVQPALV
                     LILTRLAFGEQFRCRQYAGAVLGLAGVTFTVWHELTNGIGTAAGVGFAIFALIAISLG
                     TVYQKRYCGRTNLVTSSLVQFLVAFAVVGILAVASEDGKIIWSWPLVITTAYLAIALS
                     VGSILLFYALLRAGTAHQVSSLFFLVPPTTGLLSAVLLGESLSRESIIGMFIAALGVA
                     LVSTVSSQHSGR"
BASE COUNT          840 a         1385 c         1525 g          790 t
ORIGIN      
        1 cccgggcgcg cacatcattg ccgcccgcgc cgctctgttc gcctgggcgg cccgccccgg
       61 ctttcgggcg ctgatgcagg ccgttctcga taacgcgcgc gccctggcgg agggcctgcg
      121 agaggcgggg tgccaactct acctcggcgg taccgacaca cacatgatcg tggtcgatct
      181 ccgtgcggag cccggctccg cagccgtgat cgaacggagg ctcgccggtc acggtgtgct
      241 cacgaaccgg gtgccgctgc cgcggcggcc gggtgatcgc ggcgaaggtg gcctgcggat
      301 cggaacgacg gcgatgacgc tgcgcggaat ggggccggac gatttcgccg ggctcgcccg
      361 actcatgggc cccgttctga agacaggtac ggaggcctcg gccatcggcg cgaacctttc
      421 ggatcgtgtg ttggaaatgg cgcgtgcctt tccggtgccg ccgagcttca tcggttaacg
      481 ctcactttat ttgggaggat gttcttttgc acagcggagc caatgacggc caggggccgc
      541 tcgccggcct caaggtcatc gacgccggga cgatgatcgc cggtcccctc gcggccacgc
      601 aactcgccga cttcggcgcc gatgtgatca agatcgagca gcctgccatc ggcgactcga
      661 tgcgccattg gacgccggag aaggatggca agtctctgtg gtggaaggtc atcggccgca
      721 acaagcgcct gatcactctc agcctctcga gcccggaggg gcaggccctc ttcaggcgcc
      781 tcgtcgagac ggcggacatc gttatcgaga actaccggcc cggcaccttc gagcgctggg
      841 ggctcggcta tgaggagctg tcgaggatca atcccgggat cgtcctcgtg cgcgtctcgg
      901 gcttcggcca gaccggccct tatgcgcagc gcggagggta cgggacggtg gcggaagcct
      961 tcagcggaat tccttccttt accggattcc ccgatcggcc tccgaccctg ccgggcttcc
     1021 cgctcgcgga ttcggtggcg gcaaccttcg ccgcgatgtc ggcgatgttc gcgatctatc
     1081 ggcgggacca cggcgaaggg cgcgggcagg tgatcgacgt cagcctttat gagcccctgt
     1141 tccggctcgt cgagtcgcag gtcatcggtt acgaccagct cgggatcgtc aagcagcggc
     1201 agggcaatcg gctggccgag gattctccga gaaacatcta cgagacccgg gacgggcgct
     1261 ggatcggcgt gtcggcaagc tcgcagcgca cgttcgagcg tctcgccgaa gccattggat
     1321 atccaggatt catcaaggat ccgcgctttg tcagcaacac caagcgatgc gagcatgcgg
     1381 acgagctcga tgagctgatc gcggcctggt ttaagcagaa cgactcggcg gagatcatgc
     1441 ggctgttcga ggagatgaac gttgtggcgg ggccggttct cgacatcagc gacatttttc
     1501 aggacccgca ctacgccgcg cgccaggaca tcgtcgaggt accggacgag gacttcggga
     1561 ccgttcgcat gcaatccgtc gtgccgaagt tctcggagac ccccggggcc gtgcgtcatg
     1621 ccggcgcggg gcttgggcgt gacaatcgca cggtcttcat cgaagaactc ggcctgggcg
     1681 aggacgagtt ccagcgtctc caggcatcca aagtgatctg agaggagata tgcaagaggg
     1741 atatgagggc gtttacgtcg tgggagtcgg acagactgcc tacgagaagc gcacgaccaa
     1801 gacactgcac cgaattcttt gggaggcgct cgccgaggcg gtgcattcgg cccgtctgag
     1861 ctggcagcag atcgacgggc tcggcgtcac gagcttcgct cttccgccgg acaacgtgac
     1921 gaccttggcc gagcatttcg gcatcgaggc acgctggctg ttccaagggc tttacggcgg
     1981 ggcctccggc atcatcaaca tgctgcacgg cgcccgggcg atccaggcgg gagacgccaa
     2041 ggtcgtcgcc tgcctcaccg cggacgtttt cgacgttgca agccacaacg agatgctcga
     2101 ccgcttcaac ggtccgttgc gggattacct gtcgccctat gcctttggcg gcgccaacgg
     2161 cgtcttcgcc ctccatacgc gactttacat ggaagagcac ggcgccaccc gcgaggattt
     2221 cggccggctc gccgtggcgc agcgaaagaa tgcccagcac aatccgaacg ctctctttcg
     2281 cggcaagaca ctgagcatgg aggagtacct gaacgcgcgg cagatcgcag atccgctgcg
     2341 gctgttcgat tgtgttcatc cctgcgccgg cggcgattgc atcatccttg cccacgagag
     2401 tatcgcgcga gatctcggcg tcccgctggt gaagatcctt gggggcgggg aaatgcacaa
     2461 ttaccccgcc aacgacgtgt actcgctgcg gggcggctgg gaggccttcc gcgatcgaat
     2521 gtatggccag gcgaagtgcg gtccgcagga cctgcatttc gcgcaactct acgacgacta
     2581 tccggtgatg gagttcatcc agcttgaagg catgggcatt tgcgagcgcg gcaaggtcgc
     2641 cgagtttctc cgcgagaccg acgtcaccgt gcatggcggc tttccgatca atacgggcgg
     2701 cgggcagctc tcggccgggc aggcgggagc atccgggggg atgattggcg tcgtcgaagg
     2761 tgtcctgcaa ctgcgcggcg aagctgcgga acgccaagtg gcgtgcgagc gcggcgtcgt
     2821 tgccggctac gggttggtcg cctatggccg ggggctctcg tcgagtgccg cgatcctggc
     2881 gaaagcgtaa ttgaaggagc gtcgcatgga aaagccgttg ccaaggatca ccggtgtcga
     2941 ccggcctttc tgggaggccg ccgattccgg tcggctgatg gtgcagcgct gtcagtcgtc
     3001 cgagtgcggg cgccacattc tctacccgcg ggtctgctgc ccatattgcg gaggggggga
     3061 tctatcgtgg gaagaggcgt ctggtcgtgg tcatatccgg agttacacct tcgttcatag
     3121 gccacagcac gagagcttct atgacgaggc gccgctctgc ttcgtggcgg tggcgctgga
     3181 ggaggggccg ctgttgtacg gcgaggtgcg gccgcggcca gccgaggccg agcaactact
     3241 cgatcgccgg gtgaaggtcg tgttcctgga gcatgccgcc ggccgaaagc tgccatactt
     3301 cgaattggag ccgatctcct gagaatggga gcaacccgga cttgcccgcg actccgaggt
     3361 gccgcagagc cgcgctttta cccgcctaag agaaaatgct ctctcgggca gaattgccat
     3421 gttcggggcg gttcggcact gaaccgccga gcgctggact gggatcatga gcccaagcct
     3481 tggcactctc cgggttagcg acccgaatgt tgcgatgata cggtagatac gagggccacg
     3541 cccaaggcag caatgaacat gccaatgatc gactcgcggc tgagactttc gccgagtaat
     3601 acagccgaga gcagcccggt cgtcggtgga accaggaaga agagactcga tacctggtgg
     3661 gcggtgccag cgcgaagcag agcataaaag agcaggatcg atccaaccga caaggcgatc
     3721 gcgaggtaag ccgtcgtaat aaccagcggc cacgaccaga tgattttgcc atcctcgctg
     3781 gcaacggcca agatcccgac gacggcgaag gccaccagga actggacgag gctgctcgtc
     3841 accaggttcg tgcgtccgca gtatcgcttc tggtaaaccg ttcccaggct gatagctatt
     3901 agcgcgaaga tcgcaaagcc gacgcctgcg gccgttccga tcccgttcgt cagttcgtgc
     3961 cagacagtga acgtgacccc ggcgagacca aggacggcgc ccgcatattg acggcatcga
     4021 aactgctcgc caaaggcaag gcgcgtcagg ataaggacca gagcaggttg gacgccagca
     4081 atcagagcga taacgccagg ctcggcacct gcgtcaaggg cagcgaacac tcctccaaga
     4141 tacccggcgt gaagcagggc gcccgaaacg gcaagatggc cgatctcccc tggtgttcgc
     4201 ggccagggcg cgtggaggca cagagccagc agtgccaggg ctccagctgc aagggcgaac
     4261 cgcagggata gaaacgtcat gggctcgatg tagggaagcc cgaattttgg ccctacgaat
     4321 cccgtgctcc acaggagcac gaagccaggc ggtgccaaca aggacacaat ccgtcggcgg
     4381 gcggtcgcgc cgctggcgac cggcgcgagg gtgttcggtg ttctgtggat ttggtccgac
     4441 atggcaatcg gtcaaaaccc cagcgcacag ccgtcctttc ggccgtcgga ggccccaagg
     4501 agcgtgcccc ggtcccagtc gatccagatc gcctgtgcgc
//
LOCUS       DASVDU010000097         5251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_425118, whole genome shotgun sequence.
ACCESSION   DASVDU010000097 DASVDU010000000
VERSION     DASVDU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425118"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VF226_06275"
     CDS             complement(<1..601)
                     /locus_tag="VF226_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073631.1"
                     /translation="MTGASKGIGKGIARVFATQGAKVLVVARHADQAEAAAKEIGNGA
                     SAFAGDVTRLADMEAMARTAEERNGGIDVLCANAGVFPQATLEEMSPEQWDEVIATNL
                     KGTFLTVKAAVPYLKKSGEGRIVLTSSITGPITGFPGWTHYGASKAGQLGFLRTASIE
                     LAKYGITINAVMPGNIMTEGLDGLGEDYLKTMAASIPLKR"
     gene            complement(510..1481)
                     /locus_tag="VF226_06280"
     CDS             complement(510..1481)
                     /locus_tag="VF226_06280"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073632.1"
                     /translation="MKHSTPTRLGLVLYGIVCLVFLYLPLVAVAFASVSKARYLTFPI
                     KRYAFKWYLEAATSSTVSNLVWISFKIAVIVTVVSMIIGFFGALAFARYRWRFRRSFQ
                     KWILLPIFFPQAVLGLALLLWFNGLGIIPSWKTAVVAHLVWIAPIATLIVAIRAYSFD
                     PALEEAARDMGASTRQILTEITLPLLWPGVLAAGLFAFLLSWGNFPLSLFTTGADSTL
                     PEWLYAKMMSGYSPLVPTVGTASFLVSTIVIVLGILVGRWLVGRRAARQRHRPLTRIS
                     RRKENLHADIDQGQVRHRDGSEQGYRQGDRPRLRDAGREGSGGRASC"
     gene            complement(1486..2340)
                     /locus_tag="VF226_06285"
     CDS             complement(1486..2340)
                     /locus_tag="VF226_06285"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073633.1"
                     /translation="MERLEERQGFLSSLPLGILLLLIFIAPLLVVGVFSVMPSGVFDL
                     LHMPSFENYRTFLEQGYYRSLLWSLGMAFAATVILLVICYPLAYGMAKVFGKFTLVIT
                     VGVVVSLFVSENIRLFGWVLTLMKGGMLLGTLQAWTGISMDSWLYNVPVIIFGLVYVY
                     FPFMLFPLAQGLATVPEEVRQAANDLGASRMRILFEIDLPLAMPGIVVGSLLTFVLAA
                     GAIAESKLLGGQVVIVMTQEIQSAFTYGQNWPLGSALAMILIAIIGFFAFIALGRIDL
                     DGIMGRRR"
     gene            complement(2345..3424)
                     /locus_tag="VF226_06290"
     CDS             complement(2345..3424)
                     /locus_tag="VF226_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073634.1"
                     /translation="MPSTVLRLSNVVKRFGSFTALHGIDLDIFEGEFLTIVGPSGSGK
                     TTLVRLLVGMDEPSEGTIHLNGKLINHVPANKRPTCMVFQSLALFPHRTVGQNIEFPL
                     KIRGVRSAERRERALELLDLLRLSRDYYDKGVQQCSGGERQRVALARALAFDPEILFF
                     DEPLSALDYRLRKTLEKELKDLHRRTGKTFVYITHSLEEAMVMSDRIAIMREGRIAQI
                     GSADEIYSAPRSRFVAEFMGEVNLFDVVGDGDGRLTSRDPALEINGKGGTLEAGAQAI
                     LMVRPEFIRFLAAGESADCVVRGAVVNEFALGSRIQYELRPEAGGTLTVEKLREARFM
                     GSPGDQISIGWPLSHAHLIRETAER"
     gene            complement(3513..4664)
                     /locus_tag="VF226_06295"
     CDS             complement(3513..4664)
                     /locus_tag="VF226_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HEX7073635.1"
                     /translation="MGKQGITRRSFMQQSALWTAAFAAGSSSFTGLAHAARNDELNIL
                     CWEGYNTDDVLGPFRDKHPDATVRAESGTSDPDMINKLRAGEVNVWDLINVNQPWAKQ
                     QLYPEQLIKPLDRARFEPYYELMMPGFQKYPLAYDPSGEHLLGMVQRFGPFSFVVNTD
                     KISRETAEDQGYELFFDPKMKDRYAVLTYDNWNIMHMCLAGGLNPFEELDEAGMKTFE
                     ETAKAIFSNAKLLSDDLVALNTALINGEIDAYFTGGTYTASPARYDGQTQVRAITPKS
                     GPVDGKGGVVWVELTSLVNNPNPSSLAPDFLEFVQQPEICKKVAFAEGTYNPISQMGN
                     PKVLELFDEDELDAIQWDTLEEEMARSLDYQIVASYDKLIEVYHAAKRS"
     gene            complement(4962..>5251)
                     /locus_tag="VF226_06300"
     CDS             complement(4962..>5251)
                     /locus_tag="VF226_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073636.1"
                     /translation="RRRFFLLDSPEPVDRARLTLARAGSTAAVRVFWNADVTRAFLFD
                     STVAAGLFAPGIHGLELSWIRGGPATPDVPPLYQAGRLEAPVETVRWNLGV"
BASE COUNT         1061 a         1627 c         1649 g          914 t
ORIGIN      
        1 ggcgcttgag cggaatcgag gcggccatcg tcttcagata gtcctcaccc agaccatcga
       61 gtccctcggt catgatgttg ccgggcatca ccgcattgat ggtaatgccg tatttggcaa
      121 gctcgatcga tgcggtgcgc aggaaaccga gctggcccgc cttcgaggca ccgtaatgcg
      181 tccagccggg aaagcccgtg atcggtcctg tgatcgagga ggtgagcacg atccgcccct
      241 caccgctctt cttgagatat ggcacggcgg ccttgacggt aaggaaggtc cccttgaggt
      301 tggtggcgat gacctcgtcc cattgctccg ggctcatttc ctcgagcgtc gcctgcggaa
      361 acacgcccgc gttggcgcac agaacgtcga tgccgccatt gcgctcctcg gccgtcctgg
      421 ccatcgcctc catgtcggcg agcctggtca cgtcgccggc gaaagcgctc gcgccgttgc
      481 cgatctcctt cgccgccgcc tcagcttggt cagcatgacg cgcgaccacc agaaccttcg
      541 cgccctgcgt cgcgaagacg cgggcgatcc ccttgccgat acccttgctc gctcccgtca
      601 cgatgacgga cttgcccttg atcgatgtca gcatgaagat tctcctttcg acgggatatt
      661 cgggtgagcg ggcgatgccg ctgccgcgcg gctctgcgtc cgacaagcca tcgcccgacc
      721 aagattccaa gaacaataac aatcgtggaa acgaggaacg atgcggtgcc gaccgtcggc
      781 acgagcggcg aatagcctga catcatcttc gcgtagagcc attcgggcaa cgtcgagtcc
      841 gcgcccgtgg tgaacagcga cagcgggaag ttgccccatg acagcaggaa ggcgaacagg
      901 ccggcggcaa gcacccccgg ccagagcagc ggcagggtga tctcggtgag gatctgccgg
      961 gtcgatgccc ccatgtcgcg cgcggcctcc tcgagcgcgg gatcgaagct gtaggcgcgg
     1021 atggcaacga tgagcgtcgc gatcggcgcg atccagacga gatgggcgac gacggccgtc
     1081 ttccagctcg gaataatgcc gaggccgttg aaccacagca ggagcgcgag gccgagcacg
     1141 gcctgcggaa agaagatcgg cagcaggatc catttctgga aggaccgccg gaagcgccac
     1201 cggtaacgcg cgaaggcaag cgcgccgaag aacccgatga tcatcgagac aacggttacg
     1261 atcaccgcga tcttgaacga aatccagacc agattggaga ccgtggagga ggtcgcggcc
     1321 tccagatacc atttgaatgc gtaccgcttg atcggaaagg tcaggtagcg cgccttcgag
     1381 accgaagcga acgccaccgc gacgagtggc agataaagaa aaacgaggca gacgatgccg
     1441 tataggacca gcccgagcct ggtcggggtc gagtgcttca tcgccctatc tgcgccgtcc
     1501 catgatgccg tcgagatcga ttctgccgag ggcgataaag gcgaagaacc caatgatcgc
     1561 gatcaggatc atcgcgagcg ccgagccgag tggccagttc tggccatagg tgaaggcgga
     1621 ttggatctcc tgggtcatga cgatcaccac ctgtccgccg aggagtttcg attcggcgat
     1681 cgcgccggcc gcgagaacaa aggtcagcaa cgatcccacc acgatcccgg gcatggcgag
     1741 cggcagatcg atctcgaaca ggattctcat acgcgaggcg ccgagatcgt tggcggcctg
     1801 ccgaacctcc tccggcaccg tggcgaggcc ctgggcgagc ggaaacagca tgaacggaaa
     1861 atagacatag accaggccga agatgatgac gggcacgttg tagagccagc tatccatgga
     1921 gatgcccgtc caggcctgga gcgtgcccaa cagcatgccg cccttcatga gcgtcagcac
     1981 ccagccgaac agccggatat tctcggagac gaagagactg acgacgacgc ccacggtgat
     2041 gaccagcgtg aatttcccga ataccttggc catgccgtag gcgagcggat aacagatcac
     2101 aagcaggatg accgtagccg cgaacgccat tccgagggac cacaggagcg atcggtaata
     2161 gccctgctcc aggaaggtcc ggtagttctc gaaactcggc atatggagca ggtcgaagac
     2221 gccggacggc atcaccgaga acacgccgac caccaggagc ggcgcgatga agatcaacag
     2281 cagcaggatg ccgagcggca gcgagctcag aaagccctgg cgttcctcca gccgttccac
     2341 gggttcaacg ctccgccgtc tctcgaatga ggtgggcgtg ggagagcggc cagccgatcg
     2401 agatttgatc gccgggcgag cccatgaacc gggcttcgcg cagcttctcg acggtcagcg
     2461 tgccgccggc ctcggggcgc agctcgtatt gaatgcgcga gccgagcgcg aactcgttca
     2521 cgaccgctcc cctgacaacg cagtccgcgc tctccccggc cgccagaaac ctgatgaatt
     2581 ccgggcgcac catcagtatc gcctgggccc cggcctcgag agtgcctccc ttgccattga
     2641 tctcaagcgc cggatcacgc gaggtaagcc gcccgtcccc gtcgcccacg acatcgaaga
     2701 ggttcacctc gcccatgaat tccgcgacga agcgcgatcg cggcgccgag tagatctcgt
     2761 cggcgctgcc gatctgcgcg atgcggcctt cccgcatgat ggcgattcgg tcgctcatca
     2821 ccatcgcctc ttcgagcgaa tgagtgatgt aaacgaaggt cttgccggtg cgccgatgaa
     2881 gatccttcaa ttccttttcg agggttttgc gcagccggta gtcgagcgcg gacaacggct
     2941 cgtcgaagaa caggatctcc ggatcgaagg caagcgcgcg cgcaagcgcg acccgctgcc
     3001 gttccccgcc ggagcattgc tgcacgccct tgtcgtagta atcccggctt aacctgagga
     3061 ggtcgagcag ctccagcgcg cgttcccgcc gctccgccga acgcacgccc cggatcttga
     3121 gcgggaactc gatattctgc ccgaccgtcc ggtgcggaaa cagggcgagc gactggaaca
     3181 ccatgcaagt cggccgcttg ttggccggga catgattgat aagcttgccg ttgagatgga
     3241 tggtgccttc gctcggctcg tccatcccga cgagcaggcg gaccagagtc gtcttaccac
     3301 tgccggatgg gccgacgatc gtcaggaact ctccctcgaa gatatcgaga tcgattccat
     3361 gcaatgccgt gaaggaaccg aagcgcttca cgacattcga cagccgcaga accgtagaag
     3421 gcattcagcc ttccctctcc tgttgcgcga gagaacggag gccaaggcat gcaccgcccc
     3481 tcggaaccgg aaggcggcgc atgaaaggcg gatcagctgc gcttcgcggc gtggtagact
     3541 tctatcagct tgtcgtagga agcaacaatc tgataatcga gcgagcgcgc catctcctcc
     3601 tccagcgtgt cccactggat cgcatcaagc tcgtcctcgt cgaacagctc gaggactttg
     3661 ggattgccca tctgcgaaat cgggttgtag gtgccctcgg caaaggcaac cttcttgcag
     3721 atttccggtt gctggacgaa ctccagaaaa tcgggcgcga gcgacgacgg gttcggatta
     3781 ttgacgagcg aagtgagttc cacccaaacg acgccgccct tgccgtcgac cgggcccgat
     3841 ttgggcgtga tcgcacggac ctgggtctgc ccgtcatagc gggccggcga cgcggtgtag
     3901 gtgccgccgg tgaaataggc gtcgatttcg ccgttgatga gcgccgtgtt gagcgcgacg
     3961 aggtcatccg acaggagctt ggcgttcgag aagatcgcct tggcggtctc ctcgaaggtt
     4021 ttcatgcccg cctcgtcgag ctcctcgaac gggttgaggc cgccggcgag gcacatatgc
     4081 atgatgttcc aattgtcgta agtcaggacc gcgtagcggt ccttcatctt cggatcgaag
     4141 aacagctcgt agccttggtc ctccgccgtt tcgcgggaaa tcttgtcggt gttgaccacg
     4201 aagctgaacg gcccgaagcg ctggaccatg ccgaggaggt gctcgcccga gggatcgtag
     4261 gcgagcgggt atttctggaa ccccggcatc atgagctcgt aatagggctc gaaacgcgcc
     4321 cgatcgagcg gcttgatgag ctgttccgga tagagctgct gcttggccca gggctgattg
     4381 acgttgatga ggtcccagac attgacctcg cccgccctga gcttgttgat catatcggga
     4441 tcggacgtgc cgctctcggc ccgcactgtg gcatcgggat gcttgtcgcg gaacgggccg
     4501 aggacgtcgt cggtgttgta gccctcccag cagaggatgt tgagctcgtc gttgcgggcg
     4561 gcgtgcgcca gccccgtgaa gctcgaggag cccgccgcga acgcagccgt ccaaagcgcc
     4621 gactgttgca tgaaactgcg ccgagtgatc ccctgcttac ccatgtcttc ctccctagcc
     4681 gaccggcgaa agccgacgtg gcccgccctt attctgttct gcttttcggc cgtgacctcg
     4741 tcaatcgcgg atgcttgacc tcagaattga gcagcgccgg ccactatgcg gtatagccga
     4801 ggcttccggc acctcactgt acagactacg aaaggcaacg ctcaccagtc ttgacttgaa
     4861 ggcacgcaaa cttgacagcg ccatgattcg ccgggaggtc tgaggttacc ccaagaagct
     4921 ggccgaactc agctgggagg aattggccag cttcgtgtct ttcagactcc gaggttccaa
     4981 cgcacggttt cgacaggggc ttcgagcctg ccagcctggt agagcggcgg cacgtccggg
     5041 gtggcggggc caccgcgaat ccagctcagc tcgagaccgt ggatgcccgg tgcgaacagc
     5101 cctgccgcca cggtggaatc gaagagaaat gcgcgcgtga cgtcggcatt ccagaagacg
     5161 cgaacggctg ccgtcgagcc ggcccgcgcg agcgtgagcc gagcgcgatc aaccggctcg
     5221 ggactgtcga ggaggaaaaa acgccgtcga a
//
LOCUS       DASVDU010000098         4030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1492072, whole genome shotgun sequence.
ACCESSION   DASVDU010000098 DASVDU010000000
VERSION     DASVDU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492072"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..673)
                     /locus_tag="VF226_06305"
     CDS             complement(203..673)
                     /locus_tag="VF226_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEX7073637.1"
                     /translation="MPNFLSLACRGLAIILALSLTTAIAPATDSGRYSKQKVVYHIDE
                     DGGEGDQFYLRALSNIQNHINAVGAENLTLKVVLHGDGVNLLKDAVSNLRLQGQVINL
                     RNQNVSFLVCKNTLVQRKIDPEEDLFEVYEEDIVPSGVAELSLLQQQGFTYVKP"
     gene            851..1960
                     /gene="leuB"
                     /locus_tag="VF226_06310"
     CDS             851..1960
                     /gene="leuB"
                     /locus_tag="VF226_06310"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7073638.1"
                     /translation="MPTHNLLLLPGDGIGVEVMAEVERVLAFFGKKSKANFEITTDLV
                     GGAAIDKYGTPLTDETLAKAKQADAVLFGSVGGPKWDDVPYEIRPEAGLLRLRRELDL
                     FANLRPAICYPALAEASSLKREFVEGLDILILRELTGGTYFGEPKEIVTLENGEKRAV
                     DTTIYTTHEIERIALVAFDLARKRKGKVHSAEKRNVMKTGVLWNQVMTETHKKHGQGV
                     ELHHILADNCAMQLVRNPKQFDVIVCDNLFGDILSDEAAMLTGSLGMLPSASLGAPDE
                     TGRRKALYEPVHGSAPDIAGQNKANPIASIASFGMALRYSFDMPREADLVDQAIAATL
                     ARGLRTGDIMQPNMRQVGTSEMGSAILEELERLSA"
     gene            2208..2951
                     /locus_tag="VF226_06315"
     CDS             2208..2951
                     /locus_tag="VF226_06315"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX7073639.1"
                     /translation="MRIRLTLASIAALVAAFANLNAQSLHGFRFLKLDGEYVVWSTWE
                     GEPKAANVTYAFVRSRTETPDAVNCSTMGPIDPLLQKSKLDRGAFQAEVRAAFDMWQS
                     VANITFTEIADPNEAGILIGAQLEPFGRAFTNVSYKSDSAEPRPIKRSLICFNPEQRW
                     KIGFDGNLEVYDVRYTMAHEIGHAIGLDHPMSAGQLMHHRYAERFRTLQEGDIEGVAL
                     LYGLRERPAPAKPQHEIHSVSVTQTAASE"
     gene            3043..3567
                     /locus_tag="VF226_06320"
     CDS             3043..3567
                     /locus_tag="VF226_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX7073640.1"
                     /translation="MTASEHWSSDVFREMKARDIRTVCTIPDGGLTQLLNMVKADPAH
                     RLVTLTTEEEGIGITTGCWLGGERAMIAMQSSGVGNCINALGLPTALHAPCLMLVTMR
                     GQWGEFNPWQVPMGQAARPALEAMGVKCFPVDKAAEVGETFAAAADLAFHGRLSAAVL
                     VSQRVIGAKGFGQK"
     gene            3564..>4030
                     /locus_tag="VF226_06325"
     CDS             3564..>4030
                     /locus_tag="VF226_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7073641.1"
                     /translation="MSTNGTSASGSNKIQRRPFVAELLKRRGNTLVVPGLGSPTWDTF
                     ASGDSPEYLYSWGGMGLAVPTALGVALAQPSRRVLALTGDGEMLMGIGSLAVVADQNP
                     QNLTILVLDNEHFGETGRQAGLTGNKVDLTAVARGFGIERTMTVTEEGKVGELA"
BASE COUNT          759 a         1311 c         1238 g          722 t
ORIGIN      
        1 gcgccgtcgg gaatgcgtgc aaccagctct tccagatcga tcaccacatc acctgtgggc
       61 atggccatgc tccctccaaa tcgcggcgca ctttggaccc ggcgatcctc cgacacaagc
      121 agcggatacg ccgtacttgc attgtacggc gcatccgcgg gccgactcag atttgggctt
      181 gcaaactgcg atcgccggag gttcagggct tcacataggt gaaaccctgc tgctgcaaga
      241 gcgacagctc cgcaacacct gagggcacga tatcctcctc gtagacctcg aacaggtcct
      301 cctcaggatc gatcttccgc tgcacgagcg tgttcttgca gaccaggaac gacacgttct
      361 ggtttctcag gtttatcact tgcccttgca gccgcagatt gctgaccgca tccttcagca
      421 agttcacgcc gtcaccatgc aggaccacct tcagcgtcag attttccgcg ccgacggcat
      481 tgatatggtt ctgtatgtta ctcagcgcgc gcaggtagaa ctggtcgcct tcgccaccat
      541 cctcgtcgat gtgatagacg accttctgtt tcgaatacct gccgctgtcg gttgccggtg
      601 cgatcgccgt ggtcagagat agcgcgagaa tgatcgcgag gcctctgcag gccaggctca
      661 agaaattagg catgtatctt cctccttggc cctctggtcc atgagggaga cacaaaacca
      721 cgaggccgcg cagcctgcaa ttcacgtccg tcatgacggg cgccatcgct cttgtgcgtg
      781 cttgcacaaa ccggttcagc gggctagcag tttgcgcacg acttccctaa ccagacggac
      841 tccttgagcg atgcccaccc acaatctgct gctgctgccc ggtgacggaa ttggcgtcga
      901 ggtcatggcc gaggtcgagc gcgtgctcgc cttcttcgga aagaaaagca aggccaactt
      961 cgaaatcacg acggacctcg tcggcggcgc cgcaatcgac aagtacggca ctccactgac
     1021 cgatgagacg ctcgccaagg ccaagcaggc cgacgccgtc ctgttcgggt cggttggcgg
     1081 gccgaagtgg gatgatgtcc cgtacgagat ccggcccgaa gcgggactgc tacggctcag
     1141 gcgggagctc gacctgttcg ccaatcttcg gcctgcgatc tgctatccgg cgctggccga
     1201 ggcctcctcg ctgaaacggg agttcgtcga ggggctcgat attctgattt tgcgcgaact
     1261 gaccggcggc acctatttcg gcgagccgaa ggagatcgtg acgctggaga acggcgagaa
     1321 gcgcgcggtc gacacgacga tctacaccac gcacgaaatc gaacggatcg cgttggtcgc
     1381 cttcgacctg gcccgcaagc gcaagggcaa ggtccactcg gccgagaagc gcaacgtgat
     1441 gaagaccggt gtcctctgga atcaggtcat gacggagacc cacaagaagc acgggcaggg
     1501 ggtcgagctg caccacattc tggccgacaa ttgcgccatg cagctcgtgc gcaatcccaa
     1561 gcagttcgac gtgatcgtct gtgacaacct cttcggcgac attctctcgg acgaggccgc
     1621 catgctgacc ggctcgctcg gaatgctgcc gtccgcctcg ctcggagcgc ccgacgagac
     1681 tggccgccgc aaggcccttt atgagccggt ccacggatcc gcgccggaca tcgccggcca
     1741 gaacaaggcc aacccgatcg ccagcatcgc cagcttcggc atggcgctcc gctactcgtt
     1801 cgatatgccg cgcgaggccg accttgtcga tcaggcgatc gctgccaccc tggcgcgcgg
     1861 cctgcgcacc ggcgacatca tgcagcccaa catgcggcag gtcggcacgt ccgagatggg
     1921 cagcgctatt cttgaggagc tcgagcggct ctcggcctga attcatgccc gtttgaacaa
     1981 tcggcgcgac gctgcaagct caggcgcgat ctaagcaata tcgtgtcgcc cgggatgcgc
     2041 tgacagcaat gatcgcgacg gtgggtcgct ggaacccatg acaggttgac cagcggcgca
     2101 ctgcaacttt aagatttgtt gtaaggctta ccttcggcca acagacgcgg cccggcgggt
     2161 ttccgccagg ctgcgcggtt gcctgcacca gccacctggg ggatcaattg aggatacgtc
     2221 tcaccctcgc gtcgatcgcg gccctggtcg ctgcattcgc gaacctcaac gcgcaaagcc
     2281 ttcatgggtt ccggtttttg aagctcgacg gagagtacgt cgtgtggagc acgtgggagg
     2341 gcgagccgaa ggcagccaat gtcacatacg cgttcgtccg ctcgagaacg gaaaccccgg
     2401 acgcggtcaa ttgcagcacg atgggtccga tcgatccatt gctccagaaa tcgaagctcg
     2461 atcgcggcgc gttccaggcc gaggtgcgag cggccttcga catgtggcag tcggtcgcta
     2521 atatcacctt caccgagatc gctgatccga acgaggctgg catcctcatc ggcgcccagc
     2581 tcgaaccgtt cgggcgggct ttcacgaacg tgtcctacaa gtcggatagc gctgaaccca
     2641 ggcccatcaa gcgctcgctg atctgcttca atccggagca gcgctggaaa atcggcttcg
     2701 acggcaacct cgaggtgtac gacgtccgct acaccatggc gcacgagatc ggccacgcca
     2761 tcggcctcga ccacccgatg tcggcggggc agctgatgca ccatcgctac gcggagcggt
     2821 tccgcaccct ccaggagggt gacatcgagg gcgtcgcgct gctttacggc ttgcgggagc
     2881 gcccggcgcc cgccaagccg cagcacgaaa tccattcggt ttcagtgacc cagactgcgg
     2941 ccagcgaata gtagctagca gtttgcaggt cttgacctaa ggagatgggg atgagcaaat
     3001 ccccatctcc ttttcttcgg cccctgccaa gagcgtggat tcatgactgc ctccgagcac
     3061 tggtcgtcgg atgttttccg cgagatgaag gcgcgcgaca tccgcaccgt ctgcaccatt
     3121 cccgatgggg ggctgacgca gctcctcaac atggtcaagg ccgatccggc ccaccggctc
     3181 gtgaccttga cgacggaaga ggaagggatc ggcatcacca cggggtgctg gctcggcggc
     3241 gagcgcgcca tgatcgccat gcagtcttcc ggcgtcggaa actgcatcaa cgccctcggc
     3301 ctgccgactg cgctgcacgc cccgtgcctg atgctcgtca cgatgcgcgg gcagtggggc
     3361 gagttcaatc cgtggcaggt gccgatggga caggccgcgc gtccggccct ggaggccatg
     3421 ggcgtgaagt gctttccggt cgacaaggcg gccgaggtcg gcgaaacctt cgcggcggcc
     3481 gccgacctcg ccttccatgg gcggctttcc gccgccgttc tcgtgagcca gcgtgtgatc
     3541 ggcgccaagg ggttcggaca gaaatgagca cgaatggcac ctccgcatcc ggatcgaaca
     3601 agatccagcg ccgccccttc gtcgccgagc tgctgaagcg ccgcggcaat acgctcgtcg
     3661 ttcccggcct tggcagtccg acctgggaca ccttcgcctc gggcgacagc cccgagtacc
     3721 tctactcctg gggcggcatg gggctcgcag tcccgaccgc gctcggtgtc gcgctcgccc
     3781 agccctcacg ccgcgtgctc gcactgacgg gcgacggcga gatgctgatg ggcatcggct
     3841 ccctcgccgt cgtcgccgac cagaacccgc agaacctcac catcctggtg ctcgacaacg
     3901 agcacttcgg cgagacaggt cggcaggccg ggttgacagg caacaaagtc gacctcaccg
     3961 ccgtcgcccg cggcttcggc atcgagcgca cgatgacggt cacggaggag gggaaggtcg
     4021 gcgagctggc
//
LOCUS       DASVDU010000099         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1583619, whole genome shotgun sequence.
ACCESSION   DASVDU010000099 DASVDU010000000
VERSION     DASVDU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583619"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VF226_06330"
     CDS             complement(<1..699)
                     /locus_tag="VF226_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7073642.1"
                     /translation="MNQEVFQDRDLVAAADSGPADFAPLILNRLRQGDTDSIVSYLED
                     QHPADVANLLANLPPQDASRVLRLLPLDMRADVFGYLGMEHQLQIGQALGRREFAKVV
                     SEMSADERADLFNRLPQEQQETLLPALAQAEREDIRKLASYPEGTAGAIMTSDYATLP
                     PNITAREALEVLRLAAPDKETIYNAYVVDEQRRLTGVVSLRDLILARPSAKIADIMDT
                     HVICARAEDSQTEVA"
     gene            complement(1103..1540)
                     /locus_tag="VF226_06335"
     CDS             complement(1103..1540)
                     /locus_tag="VF226_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7073643.1"
                     /translation="MYRHILVATDGSELSERAIRQAVALAQALQAKVTALHVIKPWRT
                     VAPGEIMVAFPESEYRKGAEIAAQKYLDVALDAARERNVPCDARSVEHPHPWQAILST
                     AEAAGCDLIVMASHGRKGLQALLIGSETKKVLAHSKLPVLICR"
     gene            complement(1743..>2702)
                     /locus_tag="VF226_06340"
     CDS             complement(1743..>2702)
                     /locus_tag="VF226_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073644.1"
                     /translation="LSEVTMDSSREGAVKKLNGHGNGLNGHGTGQSDDGAPQPPLSGL
                     VDELAQAMNMARESLDRPQPQPLNPTAPPLPAMAPPRTLFEDEDDDAAMPIPSTWRTP
                     PEPPKTGTLRDQLRAAGFGFATGLAVIVPVVLVMTGRLGDVSFDALFGSSDPITSKVS
                     QSAAIPQRTPERVPVQVQQRTVPTTIVTPRATPDSAPARPTASSPEPTNLAPAAATPP
                     PAAPAEAEAPPSKPSWADTIAEAKDRIQAGDIRGAREMLLPAVAANEPEAIMTLAETY
                     DPNMLAAWGIRDVTADVEQARQLYARALREGVEPARIRLRGLN"
BASE COUNT          435 a          863 c          904 g          500 t
ORIGIN      
        1 cgccacctct gtctggctgt cctcggcgcg ggcgcagatg acgtgcgtgt ccatgatgtc
       61 tgcgattttc gcgctgggcc tggcgaggat caggtcgcgc aaggagacca cgcccgtcag
      121 ccgccgctgc tcatcgacga cataagcatt gtaaatcgtc tccttgtccg gcgcggcgag
      181 ccgcaggacc tccagcgcct ctcgggccgt gatgttcggc gggagggtcg cgtagtcgga
      241 ggtcatgatc gcgccggctg tcccttccgg gtaagaggcc agcttgcgaa tgtcctcgcg
      301 ctcagcctgt gccagagccg gcaacagtgt ttcctgctgc tcttgcggca accggttgaa
      361 aaggtcggcc cgctcgtccg ccgacatctc cgagacgacc ttggcgaact ccctccggcc
      421 gagcgcctgg ccgatctgca gctggtgctc cattcccaga tagccgaaca cgtcggcgcg
      481 catgtcgagc ggcagcaggc gcagcacgcg gctcgcatcc tgcggcggca ggttggcgag
      541 aagattggcg acgtctgccg ggtgctgatc ttccaaatag gagacgatcg agtcggtatc
      601 gccctggcgc agccggttca gtatgagcgg cgcgaagtca gcagggccac tgtcggcagc
      661 ggccacaaga tcccggtcct ggaagacttc ctgattcatg gtccgcctcc tcatggctgc
      721 ccgggcgcgg gcagccggtg gcggactgga agggcttagg tgcgagccct cggagccgac
      781 acggcgagcc gtaccctacg gcagatggca gccccgacag gctgcattgt cgcgttccgg
      841 tcgatgtccc cgaagggacg actactaggc acgtccattt ctgatccaat tgatgaaacg
      901 agcggcgaac gggttcgcgc gcttgcgcgg tagatgcagt gcgctgcggc ccatgtcaac
      961 tcaatccgct gggctgcgcg gcaatcctgc agataaaaat tcgcgaaacg taaggctgtc
     1021 gcagtacggg taggccgtag tgacggcaga aggggctcac gctctattat acctgcgcac
     1081 caccgcgacg gccgtgccgg cgtcagcggc agatgagcac cggcagcttg ctgtgcgcca
     1141 gcaccttttt ggtttcgctg ccgatgagca gagcctgcag tcctttgcgg ccgtgggacg
     1201 ccatgacgat cagatcgcag ccggccgctt cggcagtcga aaggattgcc tgccacggat
     1261 gcgggtgctc caccgacctc gcgtcgcagg ggacattcct ttcccgggcg gcatccaggg
     1321 cgacatcgag gtatttttgc gcagcaattt ccgccccctt ccgatactcg gactcgggga
     1381 aggcaaccat gatttctcct ggcgccacgg tgcgccaggg tttgatgacg tgcaaagccg
     1441 tgactttcgc ctgcagcgcc tgagccagtg ctaccgcttg ccggatggcg cgctccgaca
     1501 gctccgaccc atccgttgcg accaaaatgt gacgatacac gggagcggtc ctcatcatcg
     1561 gccggatctc gccggcggcc tctctcgaac ttcggtgcag acggtgatga tggcaagcgc
     1621 ggttcatcaa ggagcaggtc gccacgtcag cggacagcat ctcgcggatg cccgcccctt
     1681 gggtttggat attgctcaat tttgccggag acagccgcag gcgcttggcc gcgcgcgctc
     1741 ggtcagttca acccccgcag ccgaatgcgg gccggctcga cgccttcgcg gagagctctc
     1801 gcgtagagct gccgagcttg ctcgacgtca gccgtgacgt cccgaatgcc ccacgcggcc
     1861 agcatgttgg gatcgtacgt ttcagccagc gtcatgatgg cttcaggctc gttggcggcc
     1921 acggcaggga gcagcatctc gcgcgcacca cggatatcgc cggcctgaat gcggtccttg
     1981 gcttcggcga tggtatcggc ccacgacggc ttggatggcg gtgcctcggc ctccgcggga
     2041 gcggccggcg gaggcgtagc agccgcaggg gccaagttgg tcggctcggg acttgatgct
     2101 gtaggtcggg ctggcgctga gtccggcgtg gccctgggcg tcacgatggt cgtcggcacc
     2161 gtccgctgtt ggacctgcac cggcactcgc tccggcgtcc tttgcgggat ggcagcagat
     2221 tgcgacacct tgctggtgat cgggtcactg ctgccgaaca aggcgtcgaa gctgacgtcg
     2281 ccaagccggc cagtcatcac gagcacgaca ggcacgatga cagccagtcc ggtcgcaaag
     2341 ccgaagccgg cggcacggag ctgatcccgg agcgtacccg tcttcggcgg ctccggcggc
     2401 gtgcgccagg tcgaggggat cggcatcgcc gcatcgtcat cctcgtcctc gaagagggtg
     2461 cgaggaggtg ccatcgcagg cagcggcggg gcggttggat tgaggggctg tggctgtggg
     2521 cggtccaggc tttcccgggc catgttcatg gcctgcgcca actcgtccac cagtccggaa
     2581 agcggcggtt gcggtgcgcc gtcgtcggat tgtccagtcc cgtggccatt cagcccattg
     2641 ccatggccat tgagcttctt gaccgcgcct tcgcgactgc tgtccatcgt aacctcactc
     2701 ag
//
LOCUS       DASVDU010000100         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1368053, whole genome shotgun sequence.
ACCESSION   DASVDU010000100 DASVDU010000000
VERSION     DASVDU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368053"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VF226_06345"
     CDS             <1..256
                     /locus_tag="VF226_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7073645.1"
                     /translation="IILPQAIPPMVPALGNYLVGLFKETPLLSAIAVLELMQTAKIIG
                     SETFRYLEPITLVGVFFLVMSLASAALIRRVEAWLRHRRD"
     gene            271..1044
                     /gene="ehuA"
                     /locus_tag="VF226_06350"
     CDS             271..1044
                     /gene="ehuA"
                     /locus_tag="VF226_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     ATP-binding protein EhuA"
                     /protein_id="HEX7073646.1"
                     /translation="MEPMVRFQGVTKRFGYLTVLKELDLDVAPNEKVAIIGPSGSGKS
                     TLLRVLMTLEPIQEGVIYVDGQPLTHMERNGKLVPADERHLRSVRGKIGMVFQNFNLF
                     PHMTALENCMEAPVRVLGLSKAEAEARAAELLKMVGLDDKMDSRPNRLSGGQQQRVAI
                     ARALAMRPKVMLFDEVTSALDPELVGEVLNVIRKLGEEHNLTMLMVTHQMGFAREFAD
                     RVCFFYDGRIAEQGPPEEFFSNPQNERTRAFLSAVLEAR"
     gene            1076..1765
                     /locus_tag="VF226_06355"
     CDS             1076..1765
                     /locus_tag="VF226_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073647.1"
                     /translation="MLLRSLRNSPAGSKARQRFEHIYNTIRDRICLLEYEPGLRLSEE
                     ELALEFKVSRTPVRRVLSRLEAEGLVESRHGVGTFVTDIDFKGLEEVYRLRMELAELI
                     GQLNPLPRTEADLDRIRRILSQCDELGTEPDARGFARLNMSLFQELSKIIGNEPLKAI
                     NESLYYLTARIWLASLPHLNLADEVAVFRREIMDVLAALEIGDLESVGHIRRTHISMS
                     FARLKRYAAAG"
     gene            1827..>2927
                     /locus_tag="VF226_06360"
     CDS             1827..>2927
                     /locus_tag="VF226_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX7073648.1"
                     /translation="MTLVHQTKDKARAMRRYSLWSVIRGGLSQQEHWTPAWRDPQPKE
                     SYDVVIVGGGGHGLATAYYLAKNHGITNVAVLEKGYIGSGNAGRNTTIVRSNYLLEGN
                     AHFYEHSLKLWEGLSRDLNYNVMFSQRGVLNLAHSDGQMDQFARRGNAMRLNGIDAEL
                     LTRDEVRKFEPNLDFSAKARFPIHGGLLQRRAGTARHDAVLWGYARGADQRGVDIIQN
                     CEVIGFRIEQGRVLGVETTRGTISAKKVGLAAAGHTTELARKAGLRLPIESHLLQAMV
                     SEPLKPLVNGVVTFGAGHFYISQSDKGGLVFGGDLDGYNSYAQRGNLPMVEHVLEIGL
                     SMLPNLSRLRLLRHWAGVMDMSMDGSPIIGLSP"
BASE COUNT          522 a          926 c          999 g          480 t
ORIGIN      
        1 catcatcctg ccgcaggcga ttccgccgat ggtgccggcg ctcggcaact atctggtcgg
       61 gctgttcaag gagacgccgc tcctgtcggc gatcgccgtg ctggagctga tgcagacggc
      121 caagatcatc ggctcggaga ctttccgcta tcttgagccg atcaccctgg tcggtgtctt
      181 ctttctggtg atgagcctgg cctccgccgc cctgatccgg cgcgtcgagg cgtggctgcg
      241 gcaccggcgg gactaggaaa ggatcaagcc atggaaccga tggtccgctt ccaaggagtg
      301 accaagcgct tcgggtacct gaccgttctc aaggagctcg atctcgacgt ggcaccgaac
      361 gaaaaggtgg caatcatcgg cccgtcgggc tccggcaagt cgacgctcct gcgggtcctg
      421 atgacgctcg agccgatcca ggaaggggtg atctatgtgg acggccagcc gctcacccac
      481 atggagcgca acggcaagct ggtgcccgcc gacgagcgcc atctgcgctc ggtgcgcggc
      541 aagatcggca tggtgttcca gaacttcaac ctcttcccgc atatgaccgc gctggagaac
      601 tgcatggagg cgccggtccg cgtcctcggc ctgtcgaagg ccgaggcgga ggcgcgggcc
      661 gcggagctcc tcaagatggt gggcctggac gacaagatgg attcgcggcc gaaccggctg
      721 tcgggcggcc agcagcagcg ggtggcgatc gcccgggcat tggcgatgcg gccgaaggtg
      781 atgctgttcg acgaggtgac ctcggcgctc gacccggaac tggtcggcga ggtgctgaac
      841 gtcatccgca agctcggcga ggagcacaac ctcaccatgc tgatggtgac ccaccagatg
      901 ggtttcgcgc gtgaattcgc cgaccgggtc tgcttcttct acgacggccg catcgccgag
      961 caaggtccgc cggaggagtt cttctccaat ccccagaacg agcgcacccg cgccttcctc
     1021 agcgccgtcc tggaggcgcg ttaggagtgc cactctgggc cccggcacaa cggcaatgct
     1081 gctgagatcg ctccggaaca gccccgccgg cagcaaggcg cggcaacggt tcgagcacat
     1141 atacaacacg atccgggacc ggatctgcct gctggagtac gagccggggt tgcggctgtc
     1201 cgaggaagag ctcgccctcg agttcaaggt cagccgcacg ccggtccggc gcgtcttgag
     1261 ccggctggag gcggaggggc tggtggaaag ccgccacggc gtcgggacct tcgtcacgga
     1321 tattgacttc aaggggctgg aagaggttta tcgactccgg atggaactgg ccgagctgat
     1381 cgggcagctc aatccgcttc cgaggacgga agccgatctc gaccgcatcc gaaggatact
     1441 gagccagtgc gacgagctgg ggaccgagcc cgacgcgcgg ggatttgcgc ggctgaacat
     1501 gagcctgttc caggaactct cgaagatcat cggcaacgag cctctgaagg cgatcaacga
     1561 gagcctctat tacctgaccg cgcggatatg gctggcgtct ctgccgcacc tgaatttggc
     1621 ggatgaggtg gccgtgttcc gtcgcgagat catggatgtc ctggcggccc tcgagatcgg
     1681 agacctcgag tccgtcgggc acattcgacg cacccacatc tcgatgagct tcgcgaggct
     1741 caagcggtac gcggcggctg gctgatgcgc ggaacgtcat cttgccggcg ccggtccgcg
     1801 gcgccgggtg gacccacgga cctcccatga ccctcgttca tcaaaccaag gacaaggcac
     1861 gtgcgatgag gcgatattct ctgtggagcg tcatccgagg cgggctctcg cagcaggagc
     1921 actggacccc ggcctggcgc gatcctcagc cgaaggagag ctacgacgtc gtcatcgtgg
     1981 gtggcggcgg ccacgggctg gcgaccgcct attacctcgc gaagaaccac gggatcacga
     2041 acgtcgccgt gctcgagaag gggtatatcg gcagcggcaa tgcggggcgc aacacgacca
     2101 tcgtccgctc caactacctc ctggaaggca acgcgcattt ctacgagcac tcgctgaagc
     2161 tctgggaggg cctgagccgg gacctgaact acaacgtcat gttctcccag cgcggcgtcc
     2221 tgaacctcgc ccattcggac ggacagatgg accagttcgc ccgccgcggc aacgcgatgc
     2281 ggctgaacgg catcgacgcc gagctgctga cccgcgacga ggtccggaag ttcgagccga
     2341 acctggattt ctcggcgaag gcccgctttc cgatccacgg cggtttgctg caacgccgcg
     2401 ccggcaccgc gcggcacgac gcggtgctct ggggctacgc gcggggggcg gaccagcggg
     2461 gcgtggacat catccagaac tgcgaggtca tcggcttccg gatcgagcag ggccgggtcc
     2521 tgggggtcga gacgacgcgg gggacgatca gcgcgaagaa ggtggggttg gccgccgccg
     2581 ggcacacaac ggagctggcg cgcaaggcgg gactccggct tccgatcgaa agccacctgc
     2641 tgcaggcgat ggtttcggag ccgctcaagc cgctcgtgaa cggcgtcgtg acgttcggcg
     2701 ccggccactt ctacatcagc cagtccgaca agggcggcct ggtctttggc ggcgacctcg
     2761 acgggtacaa ctcctacgca cagcgcggca acctgccgat ggtcgagcac gtgctcgaga
     2821 tcgggctgtc gatgctgccg aacctgtcgc ggctgcggct gctgcgccac tgggcgggag
     2881 tcatggacat gtcgatggac ggcagcccga tcatcggcct ttcgccg
//
LOCUS       DASVDU010000101        28618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1000465, whole genome shotgun sequence.
ACCESSION   DASVDU010000101 DASVDU010000000
VERSION     DASVDU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28618
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000465"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..2080
                     /locus_tag="VF226_06365"
     CDS             302..2080
                     /locus_tag="VF226_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7073649.1"
                     /translation="MRQFQAVPAVAMRALQRFVKLNGRDYSISVLLGLVTLASILPLL
                     LLGIFGLTQYVAEQRADQLNRMSRYTSALANAVDRELTGYLDLAEVLAASRFLTSGDL
                     QAFGDLARDAATKARGHIVLIDRSGQQLVNTRLPRDVGLPMTEDMASLREVIETGKPA
                     VGDLFVGAVSKKLLFVVRVPVKVDGEVRYVLSFEPEPDAMAKVLHQTYIPDGWFGGVL
                     DGKGHIIARSFLHNKFFGESASPEFVAKLTGPQGFLNSVDLEGREGLTRYQQSGLSGW
                     HVLMWAPWSVLNAPLRASVMLAVALIALTLLASLAAGFLSGRIIAEPVRQLLLAAKTL
                     GEGKPVSFEASYMREANVVGQSLVEATHNIASREQALRKSELRTRFVMRELSHRSKNL
                     LAVIQAIARQTGRASEDINEFNQQFGERLASLGRSHDLLVHGNWQGVSLADVITAQLK
                     AFIDTSEPRVTTSGAPVLLNAEAAQNIGMALHELATNASKHGALSVPSGRVKIEWNLY
                     GQGGDRRFRLSWTESGGPPVKPPQRKGFGHLVVERLVAASLRGCAELHWRPEGLVWVL
                     DVPESSILEGMHEPAEELPGEASPAS"
     gene            2197..2817
                     /locus_tag="VF226_06370"
     CDS             2197..2817
                     /locus_tag="VF226_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX7073650.1"
                     /translation="MASPRIELHKGDLPEGLSFGPSVAVDTEAMGLNPHRDRLCLVQL
                     SAGDNTAHLVQFTAGDYSAPRLKALLTDPSVLKIFHFARFDIAILKAYLGVATAPLYC
                     TKIASKLARTYTDRHGLKDLASELAGIELSKQQQCSDWGAAELTQQQLQYAASDVLYL
                     HAIKARLDAMLERENRLALAQACFEFLSARAELDLLGYDDIDVFAH"
     gene            2958..4976
                     /gene="lptC"
                     /locus_tag="VF226_06375"
     CDS             2958..4976
                     /gene="lptC"
                     /locus_tag="VF226_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7073651.1"
                     /translation="MALATDSNGRHLSAEPAPVAWSMGIDRSRAFAIAQRHSLLVRWL
                     KIILPICTVAVLSSYAIFMQRSLEVDGGKLELGPVAFSSDVLTMHNPRYEGYGKDGTR
                     FAIAARTAEQDIQREGPIRLTTIEGTIVQPNKTTTTLLATRGAFDSDANELELLDAIE
                     LQSSDGMSARLSRAKVFMKESKIVSNEPVTVEMPTGTLTGNSMVLLQKSRELTFMDGV
                     TARLKPQSRPDNAAQTDQPHSMASLIGSSDAPVNVTAPKLTVNDEHKTAVFSGNVRVE
                     QNGAILTARELQVLFDSAGSGADAGNAPAVPGTAASGRVKRIFAYDDVVITRGTDRVT
                     SAAAEFDTVAETSILTGGVTFDSGSDRRAVADRADLDFKAETVLLTGSVAVSQGPNTL
                     EGRRLYLDRKNGTMQLSSPALEGAPAGRIKARFQQSNPKPASAAASPNNGEVGGLRFR
                     TDPNAPIEIDADTLDVDDKAKTATFRGDVVVVQGDFRIRTVELVATYTGQAGVQLMQP
                     SDPEAKKPAAQLQRVQARRKVVVTSKGDQSASGDWADFDVKANTVVLGGDVTLTQGRN
                     VIRGPRLVIDMTTGLSRMETRHAQPNGTASHPGAAARGSKAPTAKGWPAGACGGRMCA
                     VFYPEDAKAFAKRRKAEDTPSRRDGAHQQGGEAASSWTSTTTPAGEPR"
     gene            5026..5880
                     /gene="lptB"
                     /locus_tag="VF226_06380"
     CDS             5026..5880
                     /gene="lptB"
                     /locus_tag="VF226_06380"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7073652.1"
                     /translation="MKLFSFHRRRTGSRRQPNGASDSSLAHYNGAEDPDLTHDEQPYA
                     ADGWLTVHDVEKSYRKRKVVRGVSLAVGRGEAVGLLGPNGAGKTTVFYMITGLVPADA
                     GAIYIDGRDATRLPMYRRARLGIGYLPQEASIFRGLTVEQNIMSVLELVEPNRKKRRE
                     KLESLLEEFNISRLRKSPSIALSGGERRRCEIARALASGPSYMLLDEPFAGIDPIAVG
                     DIQELVRHLTQRGIGVLITDHNVRETLSLIDRAYIIYDGQVLTQGHPEEIIKNEDVRR
                     VYLGDMFG"
     gene            5944..7479
                     /gene="rpoN"
                     /locus_tag="VF226_06385"
     CDS             5944..7479
                     /gene="rpoN"
                     /locus_tag="VF226_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX7073653.1"
                     /translation="MVLSARLEVRQSQQLVMTPQLQQAIRLLQYSSLELNAFVEAELE
                     RNPLLERADGEGEAVGTPISAVDDIGADASEPSESEIWQSLGEGSPEPVASLDAELDS
                     VFPDATPSDLAPGLRDSGWASLRPTNGSAHDGEFDPEAFSASEVSLKDHLLSQLPLAI
                     ADPADRLIGQFLIDMVDEAGYLTGELETIAERLGTTTERVESVLATLQTFDPPGVCAR
                     NLAECLALQLKDQGRYDPCIAKLLDNLELLAGHNLPALRKAVGVEMDELLEMIAEIKQ
                     LRPKPGLLFGSVQVQPVIPDIIVRRASDGGWHVELNAETLPRVLVNRTYYARIAKSAQ
                     RAGERSYLLDCLQSANWLVKSLDQRARTILRVAEEIVRQQDGFFTHGVQYMKPLTLRM
                     VADAVSMHESTISRVTSNKYISTPRGIFELKYFFTSSIAAAGDGDAYSSEAVRHLIRQ
                     LIDAETSETVLSDDMIVDRLKAEGIDIARRTVAKYRESMRIPSSVQRRRIKRLEAGTR
                     SVA"
     gene            8294..8761
                     /gene="ptsN"
                     /locus_tag="VF226_06390"
     CDS             8294..8761
                     /gene="ptsN"
                     /locus_tag="VF226_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX7073654.1"
                     /translation="MDLGDLLTPEGVIPSLKAKSKKQALQELSQRAAELTGLQARDLF
                     ETLLQRERLGSTGLGRGIAIPHVKLKNLKKIICLFARMEEPIDFDSLDNEPVDLIFLL
                     LAPEHASGDHLKALARISRLLREPNTIARLRAATDSAALYAVLTEPATTSEAV"
     gene            complement(8776..9573)
                     /locus_tag="VF226_06395"
     CDS             complement(8776..9573)
                     /locus_tag="VF226_06395"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7073655.1"
                     /translation="MPIWQVLLLGLVEGLTEFIPVSSTGHLLLLGHFLGFESPGKTFE
                     VLIQLGAVLAVLTVYLSRLVRLTVDLPSSPRARRFILGLVLAFVPAAAAGVALHGFIK
                     TVLFETPMLICVMLILGGIVLLYVDRIAFRPRYKDVMDFPPSLAFKIGICQAAALMPG
                     VSRSGATIIGAMLLGTDKRSAAEFSFFLAMPTMAGAFAYDLYKNWGLLHADDFTGIAI
                     GFLAAFGAAVVVVRTLLQFVSRYGYGPFGWWRIIVGGLGLGALAVLG"
     gene            9752..10444
                     /locus_tag="VF226_06400"
     CDS             9752..10444
                     /locus_tag="VF226_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7073656.1"
                     /translation="MHTLIHYRLCPASRAIRIVLAELGIEAQLVEEKPWEWRPDFLAL
                     NPSGELPVLQFDTGVLICGAYPISEYLAEEIPEHPIDGRAVPLFPGNSEERAEVRRLV
                     DWFYRKLEREVVRDLLYEKHYGRASGQITGPDLAVLRAARTNLRYHLRYISFLSDHRR
                     WLAGDEMSFADIAAAAHLSSVDYFGEVPWDEHPLAKAWYARIKSRPSFRALLADRVPG
                     LVPPPHYDDLDF"
     gene            10472..11587
                     /gene="queG"
                     /locus_tag="VF226_06405"
     CDS             10472..11587
                     /gene="queG"
                     /locus_tag="VF226_06405"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7073657.1"
                     /translation="MSQNLKDSLIAHARELGFGGVRVTTPERIGPAGERLRAFLAEGR
                     HGDMAWMATTAERRADPQVLWPDVRSIVMLGFSYAPGSDPLAALADSSHGAVSCYAQS
                     KDYHDIIKGRLKQIASAFARKSGADVKVFVDTAPLMEKPLAAAAGLGWQGKHTNLVSR
                     EHGSWLFLAAILTTAELQPDAPEPDHCGSCRRCLDACPTGAFPAPYQLDARRCISYLT
                     IEHKGHIDEEFRTAIGNRVFGCDDCLAVCPWNKFAQAAHAMQPYLRPQAAGAPIAELL
                     QLDDAAFRERFAGTPVKRTGRDRFIRNVLIAAGNSGDAGLMRYVEPLATDPSPLVRAM
                     AAWAAWRLCDTDTFRRLKARYAAKEDDPVVRAEWERD"
     gene            11736..13556
                     /locus_tag="VF226_06410"
     CDS             11736..13556
                     /locus_tag="VF226_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073658.1"
                     /translation="MRSISRRKSSTLVGLIAALTFSACTVEAGDEAAHAIAAKFAADA
                     ALSEEPPADEPAAAPAAPPILIVNSPDARAQAEKEKAAAAKEERRREAERRIAEERTR
                     KQAARAAAEKAEAERLARAQREAEEAEMLARARAEAEARKLAEQKAAEERRRAELAAK
                     EEARKAEERRIAEERERAEAARLAAARAEAERRLKAQREAEEAEMLARARAEAEERRM
                     EEERRLRELAQAKAEAERRQAAEKARKEQARLAEEERQRQAAEARRLAEISAREQERK
                     AAEAHKIDEENQRRADQERLEAIEAKHLSELAEQEIVRTSRSTRAAEFWRRFEEEERM
                     KVLEAERRAAEAQRQAAQRAAEARRAAALHQLEEEGRRRAEQARAIAELSAAAEARKA
                     SPASSTVAPHPVRPARESWADEHEKPYVRPYALGRSHAEDRIETRSPGITRVTVLLVM
                     EPGRRGIRRFNKTADPILCVGAGCYISNGAGAPSQVMRRAKAFGPVVAIGLRAGKCSR
                     SLTCVFRSVDLERPRSEVQPIDLRILRHDRRKPSIVMADPTCRTDGKRLTCSRTVVAD
                     DYRMWIVPESVAERAGAVALESAVLNGLRGDGNVARAGSG"
     gene            complement(13557..14348)
                     /locus_tag="VF226_06415"
     CDS             complement(13557..14348)
                     /locus_tag="VF226_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7073659.1"
                     /translation="MPDSKTQHLDVGTGADRRRIAVLRDAGRPPGLLWLPGFKSDMVS
                     TKATALSEWAREKGLACTRFDYSGHGQSEGRFEDGTVGRWLEETRAVFTQMTEGPVIV
                     IGSSMGGYIALLLLRQLMAEAPEEASRIKALVLIAPAWDMTEELMWKEFPEDVKREIT
                     EKGVWLRPSAYGEPYPITRELIEEGRKHLIGRQAWDPGRPVHILHGLQDPDVPWQHTV
                     ELEKNLTGGWTRVTAVPDGDHRLSRPEDLNLLCDVIGQLAETEKQ"
     gene            14385..15617
                     /locus_tag="VF226_06420"
     CDS             14385..15617
                     /locus_tag="VF226_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7073660.1"
                     /translation="MPEHRPTILQIIPRLDTGGAERTTIEMTQAIIAAGGRALVVTEG
                     GRMLPQVAEAGGEVITLNAGTKNPLSMLANARRLQRLVQREKVDLLHARSRAPAWSAL
                     VAARLCGRPLVTTYHGAYAEKGAAKRTYNSIMARGDVVIANSHYTARLIQSRYGTPAS
                     RIRVIHRGIDEASFDPEKVSTDRVDRLRAQWGVEAGQRIVLHAARLTGWKGQSVVIAA
                     AARIVQQHGPDGDWVVILAGDAQGRSDYVEELMQQIARDGLAGRVRLVGHVDDMPAAY
                     LAAHVAVVASTEPEAFGRAAAEAEAMGCPVIATDIGAAPETVKAEPLVAASEITGWLV
                     PPADIDRLAHYMRLALTLTDGERAKLGQRARVHVTQSFSLKAMQKATLCVYDDLLGTR
                     MTEVWSETKPYSRRSADA"
     gene            15747..16277
                     /gene="infC"
                     /locus_tag="VF226_06425"
     CDS             15747..16277
                     /gene="infC"
                     /locus_tag="VF226_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX7073661.1"
                     /translation="MVRGAPEREPTGPRINEAIRAREVRLIDENGQNLGIVSRFEALE
                     RASAAGLDLVEVSPDANPPVCKILDFGKFKYQEQKKAAEARKHQKIVEVKEIKMRPSI
                     DDHDYDVKMRSIKRFFDEGDKVKITLRFRGREMAHQHIGYDLLQRVKADLGDLAKVEA
                     EPRLEGRQMVMVLAPR"
     gene            16479..16679
                     /gene="rpmI"
                     /locus_tag="VF226_06430"
     CDS             16479..16679
                     /gene="rpmI"
                     /locus_tag="VF226_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7073662.1"
                     /translation="MPKLKTKSSAKKRFKMTATGKVKVQAQGKRHGMIKRTAKFIRNA
                     RGTMVLSDSDAKIVKKYMPYAR"
     gene            16745..17122
                     /gene="rplT"
                     /locus_tag="VF226_06435"
     CDS             16745..17122
                     /gene="rplT"
                     /locus_tag="VF226_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7073663.1"
                     /translation="MRVKRGTVGHAKHKKVLKAAKGYYGRRKNTIRVAKQAVERAQQY
                     AYRDRKRRKRTFRALWIQRINAAARELGTTYGQFINGLNKAGIEVDRKILADIAVRDS
                     EAFKALVEQANAAARASASAASA"
     gene            17306..18382
                     /gene="pheS"
                     /locus_tag="VF226_06440"
     CDS             17306..18382
                     /gene="pheS"
                     /locus_tag="VF226_06440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7073664.1"
                     /translation="MATFPELEKLQSELLNQIEAATDLAELERVRIAALGKKGSISEQ
                     MAKLGSLPADQRRAFGQATNAVKAAVSNALESRKAELEAAAIEKRLATEREDITLPVR
                     LGPLAEGRIHPVSQVFDEVIEIFADMGFSVAEGPDIETDDLNFTKLNIPEEHPARQEH
                     DTFYLRDGGSDGPRMLLRTHTSPVQIRTMLSQKPPIRIIAPGRVYRCDSDQTHTPMFH
                     QIEGLVIDETTHLGHLKWCLEEFCKAFFEVEEVKMRFRASHFPFTEPSMEVDIDAAAI
                     GKPGQWLEILGSGMVHPNVIRNCGLDPDVYQGFAFGMGLDRIAMLKYGIPDLRDMFSA
                     DLRWMKHYGFLAVDVPTLAGGLSS"
     gene            18551..19108
                     /locus_tag="VF226_06445"
     CDS             18551..19108
                     /locus_tag="VF226_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073665.1"
                     /translation="MLAVLNTTPAVLVAIAIAALMIAASVFIIFYHDRKHEELDQWVD
                     FSLSRETLRNAAMYYRFMAVSMLVFYVFFTISCLLLQAEGHLIFSDGKEPIYAGPIGT
                     SLFAIDLVLRGGFFDIMEHFDLGVSPIAMNRKLFWFVIYAFVFRMFYAVTLIKIAISF
                     IWIHGKIRMARQVFRTSSSQLRLFE"
     gene            19210..21639
                     /gene="pheT"
                     /locus_tag="VF226_06450"
     CDS             19210..21639
                     /gene="pheT"
                     /locus_tag="VF226_06450"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7073666.1"
                     /translation="MKFTLSWLKDHLDTNASLEELADRMSAIGLEVESIDNPAEKLAP
                     FTIARVIEAKQHPNADRLRVCQVEIAPGQAPVEVVCGAPNARTGMIGVFAPLGSYIPG
                     TGITLDKRPVRGVVSNGMLLSERELELSDNHEGIIELDPALGEHVGQRYIDVVGLNDP
                     VIEVKLTPNRPDCTGVRGIARDLAAAGLGTLKPEPKLGPVEGSFACPIEIRLEFTPET
                     ADACPVFAGRLVKDVKNGPSPAWMQNRLKASGLRPISALVDVTNYISLDRGRPLHVYD
                     ADKLKGAIRARLGRPGERFLALDGKEYEVDETMCVIADESGPLGLGGIIGGESSACTE
                     ETTNVFIESAYFDPLRTAATGRKAGVQTDARYRFERGVDPAYVIPGLDYATDLILKLA
                     GGKPSKRLVAGTPPEGRKSIAFDFRRIARLTGLAVKDSESRKVLSDLGFVVEGKGPEV
                     QVTTPSWRPDIGGRADLVEEVVRIIGLDKVPSTPIPRTHGVTRMVLTEAQRRARRARR
                     VLAGRGLVEAMTWSFIPRPQSELFGGGSEALELANPISVEMSSMRPCLLPGLLSAVQR
                     NRNRGLPNLGLFELGNAYRGIRPEDQIQIAAGVRAGAARLCGEGRHWDAPAQEATVFD
                     VKADVFATLAALGIDPNRAQIAREAPAWYHPGRSGVLRLGPKNVLAHFGEIHPSTLAE
                     LDVAGPVQAFEIFLDALPPEKRKSRARPPMVAADLLPVRRDFAFVLDESVPAGDVVKA
                     AAGADKSLIADVSVFDVFSGKSLGAGKKSVAIEVTLQPRERTLTDSEIEAVAKKVISE
                     VKRVTGGEIRG"
     gene            complement(21672..22409)
                     /locus_tag="VF226_06455"
     CDS             complement(21672..22409)
                     /locus_tag="VF226_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7073667.1"
                     /translation="MTKRENQLRQSIIDHCRWMNAIGLNQGTSGNISVRHGDEMLITP
                     SGIPYDSMTPDMIAAMPLSRSDGSYRGPLRPSSEWRIHLDIIRARPEAGAVVHAHSTY
                     ATALAMTRRGIPACHYMVAVFGGGDVRCSEYARFGTQELSDRALAALEGRNGCLLANH
                     GMLTLGPTLEKAMWLAVELETLAKQYVLSLQIGGPVLLNDAQIEETRAAMAGYGHSEA
                     AAAPAGGSASKRSRKKPATRRKSIQQS"
     gene            22604..23401
                     /gene="bcsS"
                     /locus_tag="VF226_06460"
     CDS             22604..23401
                     /gene="bcsS"
                     /locus_tag="VF226_06460"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HEX7073668.1"
                     /translation="MTFKTGAFLSAALAAAPISTALAGGDGPYVNADVYPLAIVSTYD
                     LAKDAFVSTTALYLALNGDLARDGFAVRLAGSMESYSYDRGPIEIDGDAWAADAMIGY
                     RWFRGDLVSGIFLGAEHQDHDLSPNDLLNPVRGDETGFKVAFDVKKLASAYSPFYADL
                     AASYSTAFDTYQVDGRVGFAVDRFVIGPEAWFLGDESTDVQRVGGFLKTELPPLLFTG
                     TTTELTLSAGYQFVDDDHGASTFGDEGAYGSLRMTTLVGRSFFDALR"
     gene            complement(23620..24561)
                     /locus_tag="VF226_06465"
     CDS             complement(23620..24561)
                     /locus_tag="VF226_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7073669.1"
                     /translation="MEQRNLGPSGLQVSVVGLGCNNFGMRIDLEGSRAVVHKALDLGI
                     TLFDTADTYGNKGGSETILGEILGNRRKDIVLATKFGMPMDPDRGLVGASRRYIMRAV
                     EDSLRRLKTDWIDLYQLHRADPHTPIEETLRALDDLVRDGKVRYIGCSNLPAWQVADA
                     QWTARYLNLNRFISCQDEYSLLVRDHERHLIPAMQHYGLSLLPYFPLASGMLTGKYKR
                     DQAPPPDTRFAVMTQRAGEYLTEENFAIVDRLTEFAEARGHTLLDLAFSWLVCQPVVA
                     SVIAGATKPEQVAQNVQAATWKLTPDEIAEVNQISAK"
     gene            complement(24633..25631)
                     /locus_tag="VF226_06470"
     CDS             complement(24633..25631)
                     /locus_tag="VF226_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073670.1"
                     /translation="MTKPRILLTHTPEMRRNYYGDRALAELRALGDVVLHEADEPLDA
                     AGLVQAAKGAQIIVSDRNTPGYGEIFGQLPDLVAFLRVAVDIRNVDVPAASQAGVLVT
                     HASRTWVPAVSELVVGHMISIARRIPDMVMAYRSGETAQALMGRQLHGSVAGIIGYGP
                     LGRNVAELLLAFGMTVLVNDPYVKVEREGIEQVQLDELLRRADFVVPLAVATEETENL
                     IGEPQLRAMKPTAYLVNLSRGNLIDEAALERALDERWIAGAALDVGRAPDQMPSPHLA
                     RRPDVIATPHMGGLTPEGIEGQALETAAQAREILQGKVPPGAVNADSAHRLRPFAS"
     gene            complement(25665..26477)
                     /locus_tag="VF226_06475"
     CDS             complement(25665..26477)
                     /locus_tag="VF226_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073671.1"
                     /translation="MKRVLMTGAAGGIATMIRPLVAPIYPEIVLSDVKEPKELQPNET
                     FVPAELADIASVERACEGIDGIIHLGGFSVEGPWETILQSNIIGCYNLFEAARRKGVK
                     RVVFASSNHAIGFYPRVQRIGVDVVPRPDSRYGVSKIFGEGLGSLYADKHGMRVLSIR
                     IGNVGEKPLDKRRLSIWIKPSDLVQLIRIGLEHPDLRYEVMYGASNNERSWWDNSVAF
                     KYGYRPTGRAEDFLEEAMAEQAKLPPDPIGDFYQGGPYCSAEFDGDTDQIWS"
     gene            complement(26527..27249)
                     /locus_tag="VF226_06480"
     CDS             complement(26527..27249)
                     /locus_tag="VF226_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7073672.1"
                     /translation="MRRLRPARNLTLEVVEQIAVEIRSGRLPPGTRLPTEHELMAALG
                     VSRTVIREAVSALRAEGLVISRQGAGVFVADASSIPFRINLEGLSSIDDVLHVMELRL
                     AVEVEAAALAAERATPNQVADVEAALEAIDAALEKGEFAVSEDFAFHSAVAAASGNPK
                     FSKFLDFLGRHIIPRQSVRASLDTPRDQQHYLRRIQSEHTRIFQAIRNGDAPAARRAM
                     RLHLNRSLSRYRRLAERQSAVD"
     gene            27467..28468
                     /locus_tag="VF226_06485"
     CDS             27467..28468
                     /locus_tag="VF226_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073673.1"
                     /translation="MNRREVIKIGIASVATTGLGAAGGFSALAQNKIVLKASDVHPLG
                     YPTVEAVVRMGKKLEAATNGRISVQMFPAMQLGGEKEMIEQAQVGALQLARISVGPVG
                     TVVKELNVFNMPFVFRDEEHMRKVIDGEIGREMLDKISSNPATKLIGLCWMDSGTRNI
                     YTKQPIRKPADLAGIKIRMMGNPIFVDTLNAMGGNGISMGFSELYTAMQTGVVDGAEN
                     NPPTFVTHNHHQIAKYYSLTEHLIIPEILVFSRVTWEKLSKEDQDLIKKYAAEAQQEQ
                     RKLWDEMVQKSMEKLKEAGVTIVSDVDKQAFRDAVKPVYDKYGADFADLMKRIQAVQ"
BASE COUNT         5397 a         9205 c         9059 g         4957 t
ORIGIN      
        1 tcaacgataa gtgatgcctt ccctgctttg cggcttgatc ggcatctgcc gggagacgat
       61 actggttgtc gcgccagacg gaactgaaca tgcgccttga atggccgcgc ctcgtgggcg
      121 gcaaacgggc gggaggggac caccctgcag agacaaggcg gcaggtaagt agttgtccct
      181 tgcgtctcgc ccgtaacttg cgcggctgtg ccgcaaagcg attcgcctca atcgcgttgc
      241 cttgtttgtt gtcctcagtc aaaggtcagc ctcccaaagc aaagaaatta ggaaggggcc
      301 cttgcggcag ttccaagctg ttccggccgt ggcgatgaga gcgttgcagc gattcgtgaa
      361 gctcaacggg cgggattact cgatctccgt tctgctcggc ctggtaacac tcgcctccat
      421 tctccctctt cttctgctcg ggatcttcgg cctgacgcaa tacgtggccg agcagcgagc
      481 cgaccagctc aaccgcatga gccgttacac ctccgccctg gccaatgcgg tcgatcgcga
      541 gctcaccggg tatctggacc tcgccgaggt ccttgcggca tcccggtttc tcaccagcgg
      601 agacctgcag gccttcggtg acctggccag ggacgcggcc acgaaggcgc gcggccacat
      661 cgtcctcatc gaccggtcag gacagcagct cgtcaacaca cggctgcccc gcgacgtcgg
      721 cctgcccatg acagaagaca tggcgagctt gcgcgaggtg atcgaaacag gcaagccggc
      781 cgtcggcgat ctcttcgtcg gcgcggtctc gaagaagctc ctgttcgtgg tgcgcgtccc
      841 ggtaaaagtc gatggagagg tccgctatgt cctgtccttc gaacctgagc cggacgcgat
      901 ggcaaaggtc ctccaccaga cgtacatccc ggatggctgg ttcggcggtg tacttgacgg
      961 gaaggggcac atcatcgcgc ggtcgtttct acacaataag ttctttggcg agtcagccag
     1021 ccctgaattc gtggcgaagc ttacagggcc ccaaggattc cttaacagtg tggacctgga
     1081 aggtcgggag ggccttacca gataccagca gagtggcctt agcggttggc acgtgctgat
     1141 gtgggcgccc tggtccgtcc tgaacgcgcc cttgcgcgcc tccgtcatgc tggcggtggc
     1201 actcatagct ctcacgctgc tcgcctcctt ggcggccggt ttcctgtcgg ggcgcatcat
     1261 cgctgagcct gttcggcagc tcctgctggc ggccaaaacc ctcggcgagg gtaagccggt
     1321 ctcgttcgag gcatcctaca tgcgcgaagc caacgtggtt gggcaatctc ttgtcgaagc
     1381 gacccacaat atcgcgtcgc gcgagcaagc cctgcgcaag agcgagctgc gcacgcggtt
     1441 cgtcatgcgc gagctgtcgc accgctccaa gaacttgctg gccgtgatcc aggccatcgc
     1501 gcgccagacc ggtcgcgcat cagaggacat caacgaattc aaccagcagt ttggagagcg
     1561 gctggcgagc ctcggccggt cacacgacct gctcgttcac ggaaactggc agggtgtctc
     1621 gctggcggac gtcatcacgg cccagctcaa ggccttcatt gatacgagcg aaccgcgggt
     1681 gacaacctct ggtgcgccgg tactgctcaa cgccgaagca gcacagaaca tcggcatggc
     1741 cctgcacgag ctcgccacca atgcctcgaa gcatggtgcg ctttccgtac cgtctggccg
     1801 tgtgaagatc gaatggaatc tgtatggcca gggaggtgat cgccgtttcc ggttgagctg
     1861 gaccgagtcg ggtggccctc ccgtgaagcc gccgcagcgg aagggcttcg gccacctggt
     1921 ggtggagcgg cttgtcgcag ccagtctgcg gggttgtgca gagctccact ggagaccaga
     1981 agggttggtc tgggtgctcg atgtacccga aagcagtatc ctggaaggga tgcacgagcc
     2041 ggcagaagag ttgccggggg aagcgtctcc cgctagttaa gcgcggcagc cggtgctaca
     2101 gattgcggct gctgtgcaga aatcgcgcgg tgatcagcgg cttaggcgag atgctgcaca
     2161 ctcccgagcc tagagaagtt ggatcggacg gcagccatgg ccagtccacg aatagagctc
     2221 cataaagggg acctgcccga agggttgagc tttgggccat ctgtcgcggt ggatacggag
     2281 gccatggggc tcaatccgca tcgcgatcgg ctttgcctcg tccagctctc tgccggcgac
     2341 aacacagccc atctggttca gttcaccgct ggcgattatt cggcaccgcg tctcaaggcg
     2401 ctgctcaccg atccgtcggt tctcaaaatt tttcacttcg cgcggttcga tatcgccatc
     2461 ctgaaggcat acctcggtgt ggcaaccgcg ccactctatt gcaccaagat tgcttcgaag
     2521 ctcgcgcgca cgtacacgga ccgccacggc ctgaaggatc tcgcaagcga actggcgggc
     2581 attgagctgt cgaagcagca gcaatgctcg gattggggcg cagccgagct cacgcagcag
     2641 cagctccagt atgcggcatc ggatgtgctt tatctgcacg ctatcaaggc gcgcctcgac
     2701 gcgatgctcg aacgcgagaa ccgcctcgct ctcgcccagg cctgcttcga gttcctgtcc
     2761 gcgcgcgccg aactcgattt gctcggctac gacgacatcg acgttttcgc ccactgagaa
     2821 cggcgctcga ttctttgatc ccccatcctt gtgggcctgg cgctcccggc gtgcttgcca
     2881 caggtcccaa tctctcccta ttttgccgcc agaatcaggc gaagcacgga tcgatcaatc
     2941 atccctgagc cgcacgaatg gccctagcga ccgatagcaa cggccggcac ttatcggccg
     3001 aaccggcgcc cgtggcctgg agcatgggca tcgaccgctc acgcgccttc gccatagcgc
     3061 agcggcatag cctcctggtg cgctggctga agatcatcct gcccatctgc acagtggcgg
     3121 ttctgagctc ctacgccatt ttcatgcagc gctcgttgga ggtcgacggc ggcaagctgg
     3181 agcttggacc cgtggcgttc tcgagcgacg tgctgaccat gcacaatccc cgctacgagg
     3241 gctacggtaa ggacggcacc cgcttcgcga tcgctgcgcg cacggcagag caggacattc
     3301 agcgcgaagg gccgatccgg ctcaccacca tcgaaggcac aatcgtccag ccgaacaaga
     3361 cgacaacgac gctgttggcg accaggggcg cattcgacag cgatgccaac gagctcgagc
     3421 tgctcgacgc gatcgagctc caatcgagcg acggcatgag cgcgcggctg agccgggcca
     3481 aggtcttcat gaaggagagc aagatcgtct ccaacgagcc ggtgacggtc gagatgccga
     3541 cgggcacgct taccggcaac agcatggtgt tgctgcagaa gtcgcgcgag ctgacattca
     3601 tggacggtgt caccgcgcgt ctcaagccgc agagccggcc agacaatgcg gcgcaaacag
     3661 accagccgca ttccatggcc agcctcatag gctcaagcga cgctccggta aacgtgaccg
     3721 caccaaagct gaccgtcaat gacgagcaca agacagctgt cttctccggc aacgtgcgtg
     3781 tggagcagaa cggcgcgatc ctcaccgcgc gggagcttca ggtcctcttc gacagcgcgg
     3841 gctccggagc cgatgcaggc aacgcgcccg cagtaccagg gaccgcggca tccgggcgcg
     3901 tgaagcgcat tttcgcttac gatgatgtcg taatcacgag aggtactgat cgggtcacca
     3961 gtgcggccgc ggagttcgat acggtcgctg agacgtccat tctcaccggc ggggtcacgt
     4021 tcgactccgg ctccgaccgg cgggcggttg cggatcgtgc tgacttggac ttcaaggccg
     4081 agacggtgct gctgacggga tcggttgccg tgtcgcaagg gcccaataca ttggaggggc
     4141 gccgcctgta cctcgaccgg aagaacggca cgatgcagct ctcctccccc gcactcgagg
     4201 gcgcgcccgc tggccgaatc aaagcacggt tccagcagtc gaacccgaag ccggccagcg
     4261 cggcagcttc gcctaataat ggtgaggtgg gcgggctgcg cttccgcacg gacccgaatg
     4321 cgccgatcga gatcgatgca gataccctcg acgtcgacga caaggcaaag acggccacct
     4381 ttcgcggcga cgtcgttgtc gttcagggcg atttccgtat ccggacggtc gagctggtcg
     4441 ccacctatac gggccaggcg ggggtacagc tgatgcagcc cagcgatccc gaggcgaaga
     4501 agcccgccgc acagcttcag cgggttcagg cgcgcagaaa agttgtcgtc acatccaaag
     4561 gcgaccagag tgccagcggg gactgggcag acttcgacgt gaaggcgaac accgtggtcc
     4621 tgggcggcga cgtgacgctg acgcaggggc gcaacgtcat ccgcgggccg cggctggtga
     4681 tcgacatgac caccggcctc agccgcatgg agacgcgcca cgcccagccc aacggaactg
     4741 cgtcgcatcc cggagctgca gcccgcggga gcaaggctcc gaccgctaag ggctggcctg
     4801 ccggggcgtg cggcgggcgc atgtgtgcgg tgttctaccc cgaggatgcg aaggcatttg
     4861 ccaagcgccg caaggcggag gacacgccaa gccggcgtga tggtgctcac cagcaaggcg
     4921 gcgaggctgc atcgagctgg acctctacga ccactccggc aggtgagcct cgatgaagcg
     4981 tccaaagcac acttcccagc cagccggcgt caggatctgc ggaccgtgaa gctcttctcc
     5041 ttccatcggc gccgcaccgg ctcgagaaga cagccgaacg gcgcatccga ttccagtctc
     5101 gcccattaca acggggcaga agacccggac ttgacccacg atgagcagcc ttatgcagcc
     5161 gacggctggc tcacggtgca tgacgtcgag aagtcctatc gcaagcgcaa ggtggtgcgc
     5221 ggggtcagtc tcgctgtggg acgcggtgag gcggtcggtc tcctaggacc caacggcgcc
     5281 ggcaagacta cggtcttcta catgatcacc gggctcgttc cggcagatgc cggcgcgatc
     5341 tacatcgacg ggcgcgacgc cacgcgcctg cccatgtacc gtcgcgcccg cttgggcatc
     5401 ggctatctgc cgcaggaggc atcgattttt cgtggcctga cggtcgagca gaacatcatg
     5461 tccgtgctcg agctggtcga accgaaccgg aagaagcgcc gcgagaagct cgaaagcctg
     5521 ctcgaggagt tcaacatctc gcggttgcgg aagtctccgt cgattgccct ttccggtggc
     5581 gagcggcggc ggtgcgaaat cgcgcgcgcc ctggcgagcg ggccctcgta tatgctcctt
     5641 gacgagccgt tcgccggcat cgacccgatc gccgtcggcg acatccagga gctggtccgc
     5701 cacttgaccc agcgcggcat cggcgtactc atcacggatc acaacgtccg cgaaacgctg
     5761 agcctcatcg accgtgccta catcatctac gacggacaag tcctcaccca gggtcacccg
     5821 gaagaaatca taaagaatga ggacgttcgt agagtttatc tcggagacat gttcggttaa
     5881 gcaggccttg cgcatgatga acaagggctg ccccgcgcat gggcatctgg gttaatcgcg
     5941 tcgatggtac tgtccgccag gttagaggtc cggcagagcc agcagctggt gatgacaccc
     6001 cagttgcagc aggcgattcg gctcctccag tattcgagcc tggaactcaa cgcctttgtc
     6061 gaagccgagc tcgagcgtaa tccgctgctg gagcgcgcgg atggcgaggg ggaggcagtc
     6121 ggcacaccca tctcagccgt ggacgacatc ggcgccgacg caagcgagcc atcggaatcg
     6181 gagatttggc agtctctcgg cgagggtagt cccgaacctg tcgccagtct cgatgccgag
     6241 ctcgacagcg tgttccccga cgcgaccccg tccgatctgg cccctggcct gcgcgacagc
     6301 ggatgggcct cgctgcggcc gaccaacggc tcggcacacg acggggaatt cgatccggaa
     6361 gccttctccg cttccgaggt ttccctgaaa gaccacctgc tcagccagct gcctctcgcc
     6421 atcgccgacc cggccgacag gctcatcggg cagttcctca tcgatatggt cgacgaggcc
     6481 ggctacctga ctggagagct tgagacgatt gccgaaaggc tcggcacgac gacagagcgc
     6541 gtcgagtccg tcctagccac gctgcaaaca ttcgacccac ccggcgtctg cgcccgcaac
     6601 cttgcggagt gccttgccct gcagctcaag gaccagggcc gctacgatcc ctgcatcgcc
     6661 aagctgctcg acaatctgga gctgctcgcc ggtcacaatc tgcctgcact gcggaaggcc
     6721 gtcggagtgg agatggacga gctgctcgaa atgattgccg agatcaagca gctccggccg
     6781 aagccaggtc tcctgttcgg ctccgtgcag gttcagccgg tcattccgga catcatcgtg
     6841 cgcagggcca gcgacggcgg ctggcacgtc gagctcaacg cggagacgct gccccgcgtc
     6901 ctcgtgaatc ggacttatta cgcgcgcatc gccaagtcgg cgcagcgggc cggcgagagg
     6961 tcctatctgc tggactgtct gcagtcggcc aactggcttg tgaaaagcct cgaccagcgt
     7021 gcccggacca ttctacgcgt cgcggaagaa atcgtccggc agcaggatgg gtttttcacg
     7081 catggcgttc agtacatgaa gccgcttact ttgcggatgg tggccgatgc cgtctcgatg
     7141 cacgaatcga cgatcagccg ggtcacgtcg aacaagtata tctcgacgcc gcggggcatc
     7201 ttcgagctga aatacttctt cacatcgtcc atcgcggcag ccggcgatgg cgatgcctac
     7261 tcgtcggaag ccgtccgcca tctcatccgc cagttgatcg atgccgagac gtctgaaacc
     7321 gtactttcgg acgacatgat cgtcgaccgg ctcaaggccg aggggatcga tatcgcccgg
     7381 cggacagtgg ccaagtatcg ggaatctatg aggatcccat cctctgtgca gaggaggcgc
     7441 atcaagcgcc tcgaggccgg cacgcgcagc gtcgcctgac gggggcaaaa gagcagccac
     7501 agtccaccgg gaagagagcc tgcactcccg ccgttgattg acattaccct gggtccaggc
     7561 taaccggggg cgcgggtaag tgatagtttc ggaccgtttc cggcaatata ggtcactagt
     7621 ctatccgggc gcagaaagca ttcggccgga acgaacgatt gctagccaat gtttgcgcgt
     7681 tgtttttcgg acagcagcaa atgcttggac ttgccgacaa ggagcatttt actaatggca
     7741 acgcggcagc cggcgcaaat cttcggccca cgccgtccaa tgctccctgg aaggattttc
     7801 gttctgaggg cgcaacccct ttcgggtccg tcgcgtctga ccgtcttgcc gtcgcaattg
     7861 cagaccatag aggctcatcg gtacaggcac cggggggcac ggtcgcggca cggatcctgc
     7921 ggcccgtttc ggccatgatc acttccgtca cgctcaagaa atcttgtgcg ctgcacaatg
     7981 ctacgcggag atcttcattg gcgcgaaatg ctgcgccatc gggacacaag cgtcgtaatg
     8041 cgcggcctgc gggcttgaac cggccggcct tgacgggcca tatgcttttg ttgtttcgaa
     8101 gggttgttgc ggagggatgg gctacgagtc actctccgaa gtcagttgga ccgctcgaga
     8161 ttcgagtgcc cgccggtgtc gcgggcgacg ttgagatcaa gacattcgat cggaactgca
     8221 ggcgcggacg ctgttggcgg cccgtgcctt ctttcactgg agactggcac gaccccgaag
     8281 ggatggcatc acgatggatc taggggacct gttgacgccc gagggcgtca ttccgtccct
     8341 gaaggcgaag agcaaaaagc aggcgcttca agaactaagc cagcgcgccg ccgagctcac
     8401 cggacttcag gcacgtgacc ttttcgagac actgttgcag cgcgagcgcc tgggctcgac
     8461 cggcttgggc cgcgggatcg cgatcccgca cgtcaagctc aagaacctga agaagatcat
     8521 ctgcctgttc gcccgaatgg aggagccgat cgacttcgat tcgctcgaca acgagccggt
     8581 cgatctcatc ttcctgctgc tcgcgcccga gcatgccagc ggtgatcatc tcaaggcact
     8641 cgcacggatc tcgcgcctac tgcgcgagcc gaacaccatc gcccgcctgc gagccgcgac
     8701 cgacagtgca gcgctctatg ccgttttgac ggaaccggcc acaacctccg aggccgtgtg
     8761 aactcacaag gcgcctcagc cgagcacggc caaggcgccg agccccagtc ccccgacaat
     8821 gatgcgccac caaccgaacg gcccgtagcc gtagcggctg acgaactgga gcagcgtgcg
     8881 cacaaccacg accgccgcgc cgaacgccgc gaggaagccg atggcgatgc cggtgaagtc
     8941 gtcagcgtgc agcagacccc agttcttgta gagatcatag gcgaacgcgc cggccatggt
     9001 cggcatggcc aggaagaagg agaactctgc agccgagcgt ttgtcggtgc cgagcagcat
     9061 cgccccgatg atggtggcgc ccgagcgcga gacgcccggc atgagagcag ccgcctggca
     9121 gatcccgatt ttgaaggcga gggagggcgg gaagtccatc acatccttgt agcggggccg
     9181 gaacgcgatc cgatcgacgt acagcagtac gatccctccc aggatcagca tcacgcagat
     9241 gagcatcggc gtctcgaaca gcacggtttt gatgaagccg tgcagggcca cgccggcggc
     9301 cgcggccggc acgaaggcga gcacaaggcc gagaatgaat cggcgcgcgc gcggactgct
     9361 cggcaagtcg acggtgagcc tgacgagcct ggagaggtag accgtaagca cggcgaggac
     9421 ggcgccaagc tggatcagca cctcgaacgt cttgccggga gactcgaagc cgagaaaatg
     9481 gcccaaaagc agaaggtggc cggtggagga gacgggaatg aactcggtca agccctcgac
     9541 cagcccaagc aacagaacct gccagatcgg catgcgtcta ttccatccac tctgccacgc
     9601 cgatcggcgc atctcgcgag gcatgcctcg cccgagccgc atctagcaga ttaaacgcgt
     9661 gaagtgacct gcaatgcagc aagatccgtc ccgaacttga cgaatcgaat gggtgcgaca
     9721 caacaagaag cgtccgaggt tgcatgacac catgcatacg ctcatccatt accggctatg
     9781 ccccgcttcc cgggcgatac gaatcgtcct ggcggagctc gggatcgagg cgcagctggt
     9841 cgaggaaaaa ccgtgggaat ggcggcccga cttcctagcg ctcaatccct cgggcgagct
     9901 gccggtcctg cagttcgata ccggcgtcct gatctgtggc gcgtacccta tctccgaata
     9961 cctggccgag gagatccccg agcacccgat cgacggtcgc gccgtcccgc tcttccccgg
    10021 caacagcgag gaacgcgccg aggtgcggcg gctcgtggac tggttctatc ggaagctcga
    10081 gcgggaagtg gtccgcgacc tgctctacga gaagcactac ggccgcgctt cgggccagat
    10141 cacggggccg gacctcgccg ttttgcgggc cgctcgcacc aacttgcgct accatctgcg
    10201 ctacatcagc ttcctgtccg accatcggcg ctggttggcc ggtgacgaga tgagcttcgc
    10261 cgacattgcg gcagcggcgc atctttcctc cgtcgactat ttcggagagg ttccctggga
    10321 cgagcatccc ctggcgaaag cctggtatgc ccgcatcaaa tcccggccct cgtttcgggc
    10381 gcttctcgcg gaccgggtac ccggcctcgt gccgccgccg cattatgacg acctcgattt
    10441 ttgaagccgc gcttccgttg cgcgggagtg aatgagccag aacctcaagg acagcttgat
    10501 cgcccacgcc cgcgagctcg gctttggggg cgtccgcgtt acgacgccgg aaaggatcgg
    10561 tccggcggga gagcggcttc gcgccttcct ggccgagggc cgccatggcg acatggcctg
    10621 gatggcgacg accgccgaac ggcgcgccga tccgcaggtg ctgtggccag atgtgcgctc
    10681 cattgtcatg ctcgggttca gctacgcccc tgggagcgac ccgctcgctg cgctggcgga
    10741 ctcctcgcac ggtgcggttt cctgctacgc ccagagcaag gactatcacg acatcattaa
    10801 aggccgcttg aagcagatcg catcggcgtt cgcgcgaaaa agcggtgcgg acgtcaaggt
    10861 gttcgtggac acggcgccgc tgatggaaaa gccgctggcg gcggccgcag ggcttggctg
    10921 gcagggcaaa cacaccaatc tcgtctcacg cgagcacggc tcctggctgt tcctggcggc
    10981 catcctgacc acagccgagt tgcagcccga tgcgcctgaa ccggaccact gcggctcctg
    11041 ccggcgctgc ctcgacgcct gcccgacggg ggcgttccca gcgccctatc agctcgacgc
    11101 gcggcgctgt atctcctatc tcacgatcga gcataagggg catatcgatg aggagttccg
    11161 gacggccata gggaatcgtg ttttcggctg cgacgactgc ctggccgtct gcccatggaa
    11221 caaattcgcc caggccgcgc acgcgatgca gccctatctc cgcccccagg cagccggcgc
    11281 gccgattgcc gagctcctgc agctcgatga cgctgccttc cgcgagcgct tcgccggaac
    11341 gccggtgaag cggaccggtc gggatcggtt catccgcaac gtcctgatcg ccgcaggcaa
    11401 ctcgggcgat gccggtttga tgcgctacgt cgagccgctt gccaccgatc cctcacctct
    11461 cgtacgggcg atggcagcct gggcggcctg gcgcctctgc gataccgata cgttccggcg
    11521 gctcaaggcg cgctatgccg cgaaggagga cgatcccgtg gtccgagcgg aatgggagcg
    11581 cgactgagcc gctggggccc ttgttcagca gcagttcatc tgtggtccgc gcttttgccc
    11641 cggttttcac acataagctg aaagtctgct gcacgagctc cggccccctg ccggaaggtt
    11701 ctcgcgcgtt ccttctttgc cggagcgtga tcgcgatgcg ttcgatttct cgccgcaagt
    11761 cctccactct ggttggactc atcgcggcgc tcactttctc agcctgcacc gttgaagccg
    11821 gggatgaggc cgcgcacgcc atcgctgcga agttcgccgc tgacgctgct ctttcggagg
    11881 agccccccgc ggacgagcca gcggcggccc cagccgcccc gccgatcctc atcgtgaact
    11941 cgcccgacgc cagagcgcag gccgagaagg agaaggcggc cgcagccaag gaggaacgcc
    12001 ggcgcgaagc ggaacgccgg attgccgagg aacgcacccg caagcaggcc gccagggccg
    12061 ccgctgaaaa agccgaagct gaacgtctgg cgcgtgcgca acgagaagcc gaggaagccg
    12121 aaatgctggc ccgcgcccga gccgaagccg aagcgcgcaa gctcgcagag cagaaggccg
    12181 ctgaagaaag gcgtcgagct gagcttgcgg ccaaagagga ggcccgcaag gctgaagaac
    12241 gccgtatcgc ggaggaacgc gaacgcgctg aggccgcgcg cctggctgcg gcaagggccg
    12301 aagccgagcg ccgtctgaag gcgcaacggg aagcggaaga agccgagatg ctggcccgcg
    12361 cccgcgccga agcggaagag cggcggatgg aagaggagcg gcgcttgcgt gagctcgcgc
    12421 aggccaaggc cgaggccgaa cggcgccagg ccgccgaaaa agcgaggaaa gagcaggcgc
    12481 gattggcgga agaggagagg caacgccagg ccgcagaggc caggcggctg gccgaaattt
    12541 ccgcgcgaga gcaggaacgc aaagccgcag aggcacacaa aatcgacgaa gagaatcagc
    12601 gtcgcgccga tcaagagcgt ctcgaagcca tagaggcgaa gcacctctcc gagctcgcag
    12661 agcaggaaat tgttcgcacc tcgcgcagca cgcgcgcggc ggagttctgg cgccgcttcg
    12721 aggaagaaga acgcatgaaa gtcctcgaag ccgagcgccg ggcggcggag gctcagcgtc
    12781 aagccgcgca acgcgccgca gaagcccgcc gggctgcggc cttgcaccag ctcgaggagg
    12841 aaggtcgccg ccgggccgag caggcgcgcg ccattgcgga attgtcagcg gcggcagagg
    12901 cgcgcaaagc gagcccggcc agcagcacag tggctcctca tccggtgaga ccagcccgag
    12961 aaagctgggc agacgagcac gagaaaccat acgtccggcc gtatgctctt ggcagatcgc
    13021 acgccgagga ccggatcgaa acccgcagcc ccggcatcac gcgggtcacc gttctcctcg
    13081 tcatggagcc tggccggcgc ggcatccgtc gcttcaacaa aacggcagat ccgatccttt
    13141 gtgttggggc cgggtgctac atcagcaacg gcgcgggcgc accttcgcaa gtcatgaggc
    13201 gggcaaaagc gttcgggccg gtcgtggcca tcggcttgcg cgccggcaag tgcagccgtt
    13261 cgctcacctg cgtcttccgc agcgtcgacc tcgagcggcc gcggagcgag gttcagccca
    13321 tcgatctccg catcctgcgc cacgaccgcc gcaagcccag catcgtcatg gcggacccga
    13381 cctgccggac cgatggcaaa cgcctgacct gcagccgcac ggtcgtcgcc gacgactaca
    13441 gaatgtggat cgtgccggag agtgtcgccg agcgcgccgg cgccgtggcg ctcgagtctg
    13501 ccgtactcaa cgggctccgc ggtgacggca acgtggcccg ggcagggagc ggttgactac
    13561 tgcttctcgg tttccgcgag ctgcccgatg acatcgcaga ggaggttcaa gtcttccggc
    13621 cgcgacagcc tgtgatcgcc atccggcacg gccgtcaccc gcgtccatcc cccggtgaga
    13681 tttttctcca gctcgaccgt gtgctgccaa ggcacgtcgg ggtcctgcaa tccatgcagg
    13741 atgtgcacgg gccggccggg atcccaagct tggcggccga tgagatgctt gcgaccttct
    13801 tcgatcaact cccgtgtgat cgggtaaggc tcgccatagg cggacggccg caaccagacc
    13861 cccttctccg tgatctcccg cttcacgtcc tccggaaact ctttccacat cagctcttcg
    13921 gtcatgtccc aggccggcgc gatcagcacc agagccttga tgcgcgatgc ctcctctggt
    13981 gcctcggcca tgagttgccg cagcaggagg agggcgatgt agccgcccat gctggagccg
    14041 atgacgatca ctggcccctc ggtcatctgg gtgaagacag cgcgcgtctc ctccagccag
    14101 cggccgaccg tgccgtcctc gaaccgcccc tccgactgcc cgtgaccgct gtagtcgaag
    14161 cgcgtgcagg cgagcccctt ctcgcgtgcc cactcgctca gcgccgtggc cttcgtgctc
    14221 accatgtccg acttgaagcc cggcagccaa aggagtcccg gcgggcggcc tgcgtcccgt
    14281 agaactgcaa tgcggcgccg gtccgcgccc gtcccaacgt cgaggtgttg cgtcttggag
    14341 tcgggcatcc tactgaggtc cttccattcc caacggggcc gttcgtgccg gagcatcgtc
    14401 cgaccatact gcagatcatc ccgcgcctcg acaccggcgg cgctgagcgc accacgatcg
    14461 agatgacgca ggccatcatc gcagccggtg ggcgggcgct cgtcgtcacg gaaggcggcc
    14521 gcatgctgcc gcaagtggcc gaggccggcg gcgaggtcat cactctcaac gcgggcacaa
    14581 aaaatccgtt gtcgatgctg gccaacgccc gccggctcca acgtctggtc caacgggaga
    14641 aggtcgacct gctgcatgcc cgcagccgcg cccccgcttg gagcgccctc gtcgcggccc
    14701 gcctctgcgg tcggccactc gtcacgacct atcatggcgc ctacgccgaa aagggcgcgg
    14761 ccaaacgcac gtacaacagc atcatggcgc gcggcgacgt ggtgatcgcc aactcgcact
    14821 acacggcgcg cctgatccag tcccgttacg gcacgcctgc ctcgcgcatc cgggtcatcc
    14881 accgtggaat agacgaagcg agctttgatc cggagaaggt ctcaacagac cgggtggacc
    14941 ggcttcgggc gcagtggggc gttgaggccg gacagcggat cgtgctccac gcagcccgct
    15001 tgacgggctg gaaagggcag agcgtcgtca ttgccgcagc ggcccgcatc gtccagcagc
    15061 acggtcccga tggcgattgg gtcgtcattc tcgctgggga cgcacagggc cggtccgact
    15121 acgtggagga actaatgcag cagatcgccc gggacgggct ggcaggccgc gtgcgactgg
    15181 tcggtcacgt cgacgacatg ccggccgcct atctcgcggc gcacgttgcc gtggttgcat
    15241 cgacagagcc ggaagccttc ggccgcgcag ccgcggaggc agaggcgatg gggtgccccg
    15301 tgattgcaac cgacatcggc gctgccccgg agacggtcaa agcagagccg ctagttgcag
    15361 catcggagat caccggctgg ctggtcccgc ctgctgacat cgaccgcctg gcacactata
    15421 tgcgtctcgc tcttacgctg acggatggcg aacgggcgaa acttgggcag cgcgctcgcg
    15481 ttcatgtcac gcagtcgttt tccttgaagg ccatgcagaa ggctacgttg tgcgtctacg
    15541 acgacttgct tgggacccgc atgaccgaag tatggtccga aacaaagccg tattcgagac
    15601 gcagtgctga cgcttgactt gggccacgac tttcccgatc ttcgccttcc aggacagccg
    15661 gtggacgcat ccgcgcccgc cgctttcagc gtaagctcta cgagtaggac cgaggcaaac
    15721 cagggagact gaacatccgc cgaccaatgg ttaggggcgc gccggagcgc gagcccacag
    15781 gcccaaggat caacgaggcg atccgggcca gagaggtgag actgatcgat gaaaacgggc
    15841 agaacctggg cattgtaagc cgcttcgagg cactcgagcg cgccagtgcc gcagggcttg
    15901 atcttgtaga ggtgtctccg gacgccaacc cgcccgtgtg taaaattctc gacttcggca
    15961 agttcaagta tcaggaacag aagaaggctg ccgaagcgcg caagcaccag aaaatcgtcg
    16021 aagtcaagga gatcaagatg cgcccgagca tcgatgatca cgactacgac gtgaagatgc
    16081 ggtccataaa gcggttcttc gacgagggcg acaaggtgaa gatcaccttg cggttccgcg
    16141 gccgcgaaat ggcccaccag catatcggct atgacctttt gcagcgcgtc aaggctgacc
    16201 tcggcgacct tgccaaggtg gaggccgagc cccgcctcga gggccgccag atggtgatgg
    16261 tcctggcgcc gcgctgattg accggagcgg cttgcgctgg ccgctccttt cggactataa
    16321 gcgagccgag ccgcccggcg gggcatgccg tggcggctcc attacgtttg cacgaacaga
    16381 ttgtcaccgc cgcacaccag ggccagtggc cgccgggtgt gatcgtctcg tgagagccag
    16441 gacgcggtgc gaccaacgag agaaatggag caagcaaaat gcccaagctg aagacgaaaa
    16501 gcagcgcaaa gaagcgcttc aagatgaccg cgaccggcaa ggtgaaggtt caggcgcagg
    16561 gcaagcgcca cggcatgatc aagcggacgg cgaagtttat ccgcaacgcg cgcggcacca
    16621 tggtgctgag cgacagcgac gcgaagatcg tcaagaagta catgccatac gctcgctgaa
    16681 ccgatcgatc ggacaccaaa agcctcgatc cgattccaat cccttcatct gacggagcac
    16741 gacaatgcgt gtcaaacgcg gcaccgtagg ccacgcgaag cacaagaagg ttctcaaagc
    16801 cgccaagggc tattacggcc ggcgcaagaa tacgatccgt gtcgccaagc aggcggtgga
    16861 aagggcgcag cagtacgcct atcgcgatcg caagcggcgc aagcgcacat tccgggccct
    16921 ttggatccag aggatcaacg ccgctgcccg cgagctcggc accacctacg gccagttcat
    16981 caacggactg aacaaggctg gtatcgaggt cgaccgcaag atactggcgg acatcgcagt
    17041 acgggattcc gaagcgttca aggcactcgt cgaacaggcg aatgcggccg cgcgagcatc
    17101 cgcttcggcg gcatcggcct gagaataccg ggcacacttc tttggcctct tccatgggaa
    17161 gaggtcggtc cgcggggccg ggagagcggt taaacggctg caatcccaga gcccccttca
    17221 ccccgaccct ctccctacgg gggagcggga gaaccggagc gagcacaact ctggttcgga
    17281 ttgattgcgg gggcagaggc agacgatggc gacattcccc gaactcgaga agctccagtc
    17341 cgagctgctg aaccagatcg aggccgcgac cgatttggcc gagctcgagc gagtgcggat
    17401 cgccgcgctc gggaagaagg gcagcatctc cgagcagatg gccaagctcg ggtcgttgcc
    17461 ggccgaccag cggcgggcgt tcgggcaggc cacgaatgcc gtgaaggcag ccgtctccaa
    17521 tgctctcgaa tcgcgcaaag cggaactgga agcggcggca atcgaaaagc gcctcgccac
    17581 ggagcgagag gacattaccc tgccggtccg gctcggaccg ctggctgagg gccgcatcca
    17641 tccggtctcg caagtgttcg acgaggtcat cgagatcttc gccgacatgg gcttctcggt
    17701 cgccgaaggt cccgacatcg agacggacga cctcaacttc accaagctca atatcccgga
    17761 ggagcacccc gcgcggcagg agcacgacac cttctacctg cgtgacggtg gctcagacgg
    17821 cccgcggatg ctgctgcgca cccacacgag cccggtacag atccggacca tgctgagcca
    17881 gaagccgccg atcaggatca tcgcgcccgg gcgcgtctac cgctgcgaca gcgaccagac
    17941 gcacacgccg atgttccatc agatcgaggg tctggttatc gacgagacca cccatttggg
    18001 ccatctcaaa tggtgcctgg aggagttctg caaggcgttc ttcgaggtgg aggaggtgaa
    18061 gatgcgcttc cgcgcctcgc acttcccgtt caccgagccg tcgatggagg tggacatcga
    18121 cgccgccgct atcggcaagc cggggcagtg gctcgagatc ctcgggtcgg gcatggtgca
    18181 tccgaacgtc atccgcaatt gtgggctcga tccggacgtc tatcagggct tcgccttcgg
    18241 catgggtctc gaccgtatcg ccatgctcaa atacggcatc ccggacctgc gcgacatgtt
    18301 ttccgctgac ctgcgctgga tgaagcacta cggtttcctc gccgtcgatg tgccgacgct
    18361 ggccggcgga ctgtcgtcct gacaatgccg ctcatcttcg gatccgggta actttcctca
    18421 ccctgacccg gtaagatgca ggctggtctg gcctccgttc tcgcctccgt cggggcctct
    18481 atggcactgc aattctggtc cggccgtgcg gggggatacc gttgcggcat cggcttgaag
    18541 gggcgccgag atgcttgcag tcctgaacac cacgcccgcc gtgctggtcg caatcgccat
    18601 cgcggccctg atgatcgcgg cgtcggtgtt catcatcttc tatcacgacc gcaagcacga
    18661 agagctcgac cagtgggtcg acttctcgct cagccgcgag acgctgcgca atgcggccat
    18721 gtactaccgc ttcatggccg tctcgatgct ggtgttctac gtattcttca cgatcagctg
    18781 tctgctgctg caggccgaag gccacctcat cttttccgac ggcaaggagc cgatctacgc
    18841 ggggcccatc ggcacatcgc tctttgccat cgatctcgtc ctgcggggcg ggtttttcga
    18901 catcatggag cacttcgacc tgggcgtgtc gccgatcgcg atgaaccgga agcttttctg
    18961 gttcgtcatc tatgccttcg tcttccgcat gttctacgcg gtgacgctca tcaagatcgc
    19021 catctcgttc atctggattc acgggaagat ccggatggcg cgccaggtgt tcaggacgag
    19081 cagcagccag cttcgcctct tcgagtgacc tgccccttgg cggccggcac gcaaaagggg
    19141 taagacgcac acgccaaaac agccccgggc cgaccggggg atagtcaaac ccgcgggacg
    19201 aattacgaaa tgaaattcac gctgtcctgg ctcaaggacc atctcgatac caacgcttcc
    19261 ctggaggagc tcgccgaccg gatgtctgcg atcggcctcg aggtggaaag cattgacaat
    19321 cccgcggaga agctggcccc ctttaccatc gcgcgcgtga tcgaggccaa gcagcatccg
    19381 aatgcggacc gcctgcgcgt ctgccaggtg gaaatcgcgc cgggccaagc ccccgtcgag
    19441 gtcgtctgcg gcgcgccgaa cgctcgcaca ggcatgatcg gcgtgtttgc gccgctcggc
    19501 tcgtacatcc cgggcacggg catcacgctg gacaagcgtc ccgttcgcgg cgtggtgtcc
    19561 aacggcatgc tcctctccga gcgcgagctg gagctgtcgg ataatcacga gggcatcatc
    19621 gagctagacc ccgccctcgg cgagcacgtc gggcagcgct acatcgacgt cgtcggactc
    19681 aatgacccgg tgatcgaggt caagctgacc cccaaccgcc ctgactgcac gggcgtgcgc
    19741 ggcatcgccc gcgatctggc cgccgccggt ctcggcacct tgaagccgga gcccaagctc
    19801 ggtcccgtcg aggggagctt cgcctgcccg atcgagatca ggctcgagtt cacgcccgag
    19861 accgcggatg cctgccccgt attcgcggga cgtctcgtca aggacgtgaa aaacgggcct
    19921 tcgccggcgt ggatgcagaa ccgcctcaag gcgtctggcc tccggccgat cagcgccctg
    19981 gtcgatgtaa ccaactacat ttcgctcgac cgcggccgcc cgctccacgt ctacgacgcg
    20041 gacaagctca aaggcgccat ccgggcgcgg ctcgggcgtc cgggtgagcg cttcctggcg
    20101 ctcgacggca aggaatacga ggtcgacgag accatgtgcg tgatcgccga cgagagcggc
    20161 ccactcggtc tcggcggcat catcggcggc gagagcagcg cgtgcacgga ggagaccacc
    20221 aacgtcttca tcgagagcgc ctacttcgat ccgctccgca ccgctgccac tggccgcaag
    20281 gccggcgtgc agacggacgc gcgctaccgg ttcgagcgcg gcgtcgatcc ggcctatgtc
    20341 ataccgggcc ttgattatgc gaccgacctg atcctgaaac tcgcaggcgg caagccgtca
    20401 aagcggctcg ttgcgggaac accgcccgaa ggtcgcaaga gcatcgcgtt cgacttccgc
    20461 cgcatcgcgc gcctgaccgg ccttgccgtc aaggattccg agagccgaaa ggtgctctcc
    20521 gatctcggct ttgtcgtcga gggcaagggt ccggaggtgc aggtcacgac gccgagctgg
    20581 cgtccggaca ttggcgggcg tgcggatctc gtcgaggagg tcgtccgcat catcggcctc
    20641 gacaaggtgc cgtcgacgcc catcccacgt acgcacggcg tgacgcgcat ggttctgacg
    20701 gaagcgcaac ggcgtgcgcg ccgcgctcgc cgcgtcctgg ccggccgcgg gctcgtggag
    20761 gcgatgacct ggtcgttcat cccgcgcccg cagtcggagc tgttcggcgg cggctcggag
    20821 gcgctggagc tcgccaatcc gatctcggtc gagatgtcct ccatgcggcc atgcctcctg
    20881 ccggggctgc tttcggccgt gcagcgcaac cgcaatcgcg ggctgcctaa cctgggtttg
    20941 ttcgagctcg gaaacgccta ccgtggcatt cggccggagg atcagatcca gatcgcggcc
    21001 ggcgtccgcg ccggtgcggc gcggctctgc ggagagggcc ggcactggga cgcgccggct
    21061 caggaagcca ccgtgttcga tgtgaaggcc gacgtcttcg cgacgctggc ggcgctcggc
    21121 atcgatccga accgcgcgca aatcgcccgc gaggccccgg cctggtatca cccgggccgc
    21181 tcaggagtcc tgcggctggg cccgaagaac gtgctcgccc acttcggcga gattcatccg
    21241 agcacactgg ccgagctgga cgtggcgggc cccgtccagg cgttcgagat tttcctggat
    21301 gcgttgccgc ccgagaagcg caagtctcgc gcccgcccgc cgatggtggc ggcggacctg
    21361 ctaccggtgc gccgcgactt cgccttcgtg ctcgacgagt cggtgccggc aggcgacgtg
    21421 gtgaaggccg cggccggggc ggacaaatcg ttgatcgccg atgtctcggt attcgacgtg
    21481 ttcagtggca agagcctggg ggccggcaag aagtcggtgg cgatcgaggt cactttgcag
    21541 ccgcgcgagc gcacgttgac ggactctgaa atcgaggccg tcgcgaagaa ggtgatctcc
    21601 gaagtcaagc gcgtgaccgg cggcgagatc cgcggctgag cagaagcccc gattctcgcc
    21661 cgtcccgccc ttcagctttg ttgaatcgat ttgcgacggg tcgcgggctt cttgcggctt
    21721 ctcttgctgg ctgatcctcc ggctggcgcc gcggcggctt cggagtggcc gtagccggcc
    21781 atggcggcgc gggtctcctc gatctgcgcg tcgttcagca gcaccggtcc accgatctga
    21841 agactcagga cgtattgctt ggcgagcgtc tcgagctcca cggcaagcca catggctttc
    21901 tccagcgtcg gccccaaggt cagcatgccg tggttggcca gcagacagcc attccggccc
    21961 tcgagggccg cgagcgctct atccgacagc tcttgcgtgc cgaagcgcgc gtattccgag
    22021 cagcgcacat ctccgcctcc gaacacagcg accatatagt ggcaggccgg aatgccccgg
    22081 cgcgtcatcg ccagcgccgt cgcgtaggtg gagtgcgcgt ggacgacggc gccggcttct
    22141 ggccgggcgc ggatgatgtc caaatggatg cgccattcgg acgatggccg cagcggtccg
    22201 cggtaggacc catccgaccg actgagcggc atcgcggcga tcatgtccgg ggtcatgctg
    22261 tcgtagggaa tgcccgatgg tgtaatcagc atctcatcgc cgtggcgcac ggagatattg
    22321 ccggaggtgc cctggttgag accgatggca ttcatccagc ggcagtgatc gatgatggac
    22381 tggcggagct ggttttcgcg cttggtcatg aattcttcga tgggaacggc tgggttgttt
    22441 gagcgcctgt tcttaataca gctttcagga cgcctctaat ctgtggcgcc gaggacccag
    22501 cgtaccgcca atcaccctcg tgggaccgcg ctcatgattc gcgatgcatc tcaagcatga
    22561 tacgattccg tcgcttcgga attacaaacg gcgggcgagc ggaatgacat tcaagactgg
    22621 cgcctttctt tcggcggcac tggccgcggc accaatttcg accgctctgg ccggcggcga
    22681 tggaccctat gtcaacgccg acgtctatcc gctcgctatc gtcagcacct acgatctggc
    22741 gaaagatgcg ttcgtctcca cgaccgcgct ttatctggcg ctgaatggcg acctggcgcg
    22801 ggacggcttc gctgtcaggc tggccggcag catggagtcg tacagctacg atcgagggcc
    22861 gattgaaatc gacggcgatg cctgggccgc cgatgcgatg attggctatc gctggttccg
    22921 cggtgatctt gtctccggga tcttcctcgg tgccgagcat caggaccacg atctgtcgcc
    22981 gaacgacctc ttgaatcccg tgcgagggga cgaaacgggc ttcaaggtcg cgttcgatgt
    23041 caagaagctc gcgtcggcct actcgccatt ctacgccgac ctcgccgcga gctattcgac
    23101 ggctttcgac acctatcagg tcgacggccg tgtcggtttt gccgtcgacc gcttcgtcat
    23161 cggaccagag gcttggttcc ttggcgacga gtcgaccgat gtgcagcgcg tcggcggatt
    23221 cctgaaaacc gagctcccgc cacttctgtt caccggcacg acaaccgagc tgacgctctc
    23281 ggccggatat cagttcgtcg atgatgacca cggagcatcg accttcggcg acgagggcgc
    23341 ctacggcagc ctgaggatga cgaccctcgt cgggcggagc ttcttcgacg cgctccgcta
    23401 gagcttttcc gattcaggcg gaaacgccaa cagcaggcac ccgcttcacc tctcccatgg
    23461 ggagaggtcg gcccgaaggg ccgggtgagg ggctgcaaca atcgccaccg gcggaacccc
    23521 ctcacaccga ccctctcccc accatggaga gggagattgg gcgcgagctt gacttggcgt
    23581 gcttctgtcc aaatcggaga tgcgctaagc gatctccgcc tacttggcgg atatctggtt
    23641 gacctcggcg atctcatccg gggtcaattt ccatgttgcc gcctggacgt tctgcgccac
    23701 ctgctcgggc ttcgtcgcgc cggcgatcac gctggcaacg actggctggc agaccagcca
    23761 actgaatgcc aggtccagca gcgtgtggcc gcgcgcctcg gcaaactcgg tcaggcgatc
    23821 aacgatcgcg aagttctcct ccgtcagata ctccccggcg cgctgcgtca tgacggcgaa
    23881 gcgcgtgtcc ggcggcgggg cctgatcgcg cttgtacttg ccggtcagca tgcccgaagc
    23941 gagcgggaaa tagggcagca ggctcagccc gtaatgctgc atggccggga tcaggtggcg
    24001 ctcgtgatca cggaccagca gactgtactc gtcctggcac gatatgaacc ggttcaagtt
    24061 cagatagcgc gcagtccact gtgcatcggc gacctgccag gcgggcagat tcgagcagcc
    24121 gatgtagcgc accttgccgt cccggacgag gtcgtcgagc gcgcgcagcg tttcctcgat
    24181 cggcgtgtgc ggatctgccc ggtggagctg atagagatcg atccaatcgg tcttcagccg
    24241 ccgcaggctg tcctccacgg cccgcatgat gtagcgccgc gaagccccca ccagtccccg
    24301 gtccggatcc atgggcatcc cgaacttcgt ggccagcaca atgtccttgc ggcgattccc
    24361 gaggatttca ccgagaatgg tctccgatcc gcctttgttg ccgtaagtgt cggcggtgtc
    24421 gaaaagcgtg atcccgaggt cgagcgcttt gtgaaccacc gcacgcgagc cttcgaggtc
    24481 gatgcgcatg ccaaagttgt tgcaccccag cccaaccact gagacctgca gacccgacgg
    24541 cccaagattg cgctgttcca ttggcaccct ccgttcgagc tgcgcaagaa agctcaccca
    24601 acaacgccga cctgccgggc tggttcagac agtcacgatg cgaagggccg gagccgatgg
    24661 gcgctgtcgg cattgactgc ccccggcggc accttgccct gcagaatctc gcgagcttgc
    24721 gccgctgttt cgagcgcctg cccctcgatg ccttctggtg tgaggccacc catgtgcggt
    24781 gtcgcgatga cgtcggggcg gcgcgccagg tgaggtgagg gcatctgatc cggtgcccgg
    24841 ccgacgtcga gcgcagcgcc ggcaatccag cgctcgtcga gcgcacgctc gagtgccgct
    24901 tcgtcgatca gattgccgcg gctgaggttc acgaggtaag cggtgggctt catggcccgg
    24961 agctgcggct cgccgatcag gttttccgtc tcctccgtgg cgacggcgag cggcaccacg
    25021 aagtcggccc gacgcaacag ctcgtccagc tgaacctgct cgatgccctc cctctcgacc
    25081 ttcacgtagg gatcgttgac gagcaccgtc atgccgaacg ccaacagaag ctccgccacg
    25141 tttcgtccga gagggccgta accgatgatg ccggcaacgc tgccgtgaag ctgccgcccc
    25201 atcagcgctt gcgccgtctc gccagaccgg tacgccatca ccatgtccgg gatacgccgg
    25261 gcgatgctga tcatgtggcc gacgacgagc tcgctgaccg ccggcaccca cgtacggctg
    25321 gcatgcgtga caagcacgcc agcctggctc gcggcaggaa cgtcgacgtt gcggatatcg
    25381 acggccacgc ggaggaaggc gacgaggtcc ggcagttggc cgaatatctc gccgtatccg
    25441 ggcgtgttgc gatcactgac gataatctgc gcgcccttcg ccgcctgcac cagccctgcg
    25501 gcatcgagcg gctcatccgc ctcatgcagg acgacatcgc caagcgcccg caactccgcg
    25561 agcgctctgt ctccgtagta gttccggcgc atctccggcg tgtgcgtcag cagaatgcga
    25621 ggtttggtca tgggaaacag cgggcctctc gcatgctggc ggcttcagct ccagatctga
    25681 tcggtgtcac cgtcgaactc cgcgctgcaa tagggaccac cctggtagaa atcgcctatc
    25741 ggatctggcg gcagcttcgc ctgctcggcc atggcttctt ccaggaaatc ctcggcgcgg
    25801 ccggtcggac gatagccgta cttgaatgca accgaattgt cccaccagga ccgctcattg
    25861 ttcgaggccc cgtacatcac ctcgtaacgc aggtcaggat gctccagccc gatgcggatg
    25921 agctgcacga ggtcggaagg cttgatccag atggagagcc gccgcttgtc cagcggcttt
    25981 tcgccgacgt tgccgatgcg gatcgagagc acgcgcatgc catgcttatc ggcataaagt
    26041 gaccccaggc cctctccgaa aatcttgctg acgccgtagc ggctgtctgg ccgcggaacg
    26101 acgtcgacgc cgatccgctg gacgcgcgga tagaacccaa tggcgtggtt cgacgaggcg
    26161 aagacgacgc gcttcacgcc ctttcggcga gccgcctcga acaggttata gcagccgatg
    26221 atgttcgatt gcagaatggt ctcccacggc ccctcgaccg aaaagccgcc gaggtgaatg
    26281 atgccgtcga ttccttcgca agcgcgctcg acgcttgcaa tgtcagccaa ctcggcagga
    26341 acgaacgtct cattgggctg aagctctttc ggctccttga cgtcgctcag cacgatttcg
    26401 gggtaaatgg gagccacgag cggacggatc atcgtggcga tgccacccgc cgctccggtc
    26461 atcaaaacac gcttcatggg ctcgtctcct ctttgccagc ttggatgact gccccatctg
    26521 ccgtcctcaa tcaacagccg actgccgctc agcgaggcgg cgatagcgag aaaggctgcg
    26581 gttcaggtgt agccgcatgg cccgccgcgc cgcgggcgca tcgccgttcc gtatcgcttg
    26641 gaaaatccgt gtgtgctcgc tctgaatgcg ccgcaggtaa tgttgctgat cacgcggcgt
    26701 atcgagcgat gcacggacgc tctggcgcgg gatgatatgc cggccgagga aatccaggaa
    26761 cttgctgaac ttcgggttgc cactcgccgc cgccacggcg ctgtggaatg caaaatcctc
    26821 actcacggca aactcgccct tttcgagcgc ggcgtcgatg gcctcgagcg cggcttccac
    26881 atcggcgacc tggtttggcg tcgcccgttc ggcagccagt gcagcagcct cgacctctac
    26941 ggccagcctg agctccatga cgtgaaggac atcgtcgatc gagctcagcc cctcgaggtt
    27001 gatgcgaaac gggatgctgc tcgcatcggc gacgaaaacc ccggcgccct ggcgcgaaat
    27061 cacgagacct tccgcccgga gtgctgacac agcttcccgg ataacggtcc ggctcacgcc
    27121 caaggcagcc atgagttcat gctcggtggg gagccgcgtt ccaggaggca ggcgcccgct
    27181 acggatctcc acagcaatct gctcgacgac ctccaacgtc agattgcgcg ccggccgcag
    27241 cctccgcata agtgccagct tgtcaccacg aggtgacggt gatacgtttt caagttgtcg
    27301 gatcatccta caacttactt aacactggtc cgaccgcact gcaaggcgca cagcgcgaag
    27361 aacgtattcg gaggagcggc cagacccgga tatcccatat tccattcgcc agatcagccg
    27421 gagtttcttc ggccaagagc atcaaatcac tcaggaggaa gcgcgaatga acagacgtga
    27481 agtcatcaaa atcggtattg cctctgtggc gactaccggc ctcggcgctg cgggcggttt
    27541 ttccgcgttg gcgcagaaca agatcgtcct caaagcatcc gacgttcacc cgctcggcta
    27601 tcccacggtc gaagccgtcg tccgcatggg caaaaagctc gaagctgcaa cgaacgggcg
    27661 aatttcggtg caaatgttcc ccgccatgca gcttggcggc gagaaggaaa tgatcgagca
    27721 ggctcaagtc ggtgccctgc aactggctcg catcagcgtc ggcccggtag gcaccgtcgt
    27781 caaggagctc aacgtcttca atatgccctt cgttttccgt gacgaagagc atatgcgcaa
    27841 ggtcatcgat ggcgagatcg gccgggaaat gctggacaag atttcgtcca accccgccac
    27901 caaattgatc ggcctttgct ggatggattc cggcacacgg aacatctaca cgaagcagcc
    27961 catccgtaag cccgcggatc tcgccggcat caaaattcgt atgatgggta acccgatctt
    28021 cgtggacact ctgaatgcca tgggcggcaa tggaatttcg atgggattca gcgagctcta
    28081 caccgccatg cagacgggcg ttgtcgatgg cgccgaaaac aacccaccga ctttcgtcac
    28141 ccataatcac catcagattg ccaaatatta ctcgctcacc gagcatctga tcattcccga
    28201 gattctcgtt ttctcgcgcg tgacctggga gaagctgtcg aaagaggatc aggatctcat
    28261 caagaagtat gccgccgagg cgcagcaaga gcagcggaag ctctgggatg agatggttca
    28321 gaagtcgatg gagaagctca aggaggctgg cgtcaccatc gtgtcggacg tcgacaagca
    28381 ggccttccgc gatgctgtta agccggtcta tgacaaatac ggcgcggatt tcgcagattt
    28441 gatgaagcga atccaggctg ttcagtaaga acaagcccgc cttccgccca atttgagccg
    28501 gcggaggcgc gctcgccaca gctgcagcga agagagtccc agggctctcg tcatctcggc
    28561 ctctccttca tcggaatcgg attgcatgtt cgcttactac gtgagggcga tggacgtc
//
LOCUS       DASVDU010000102         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1396590, whole genome shotgun sequence.
ACCESSION   DASVDU010000102 DASVDU010000000
VERSION     DASVDU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396590"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..156
                     /locus_tag="VF226_06490"
     CDS             <1..156
                     /locus_tag="VF226_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX7073674.1"
                     /translation="VPTVVMVSCNPATAGRDARILIDGGYRLESLTPIDQFLYSAHLE
                     LVAVFRR"
     gene            complement(160..870)
                     /locus_tag="VF226_06495"
     CDS             complement(160..870)
                     /locus_tag="VF226_06495"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX7073675.1"
                     /translation="MARYERIVILTGAGLSAESGLATFRDKDGIWSKYDLTEVATPEA
                     FARDPAKVHDFYNQRRRGLRDVAPNAAHRALARLEREYPGVVTIVTQNIDALHEAAGS
                     RRLIHMHGELLKALCARCEARHPWSDDLSVDVVCPGCGKPGGMRPDVVWFGEMPYRMD
                     EIYELIAEADLFASIGTSGTVYPAAGFVREARATGAHTVELNLEPSEGVSLFAEAIHG
                     PATRIVPEWVDRLLAQAE"
     gene            1023..2066
                     /locus_tag="VF226_06500"
     CDS             1023..2066
                     /locus_tag="VF226_06500"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7073676.1"
                     /translation="MSGALGMSAQLASNAIRFGWYYGLNQLLDRRARRLSARPSYRPE
                     RPVPTRQELLADLGRLFLEDAARVRDGIYPAGEEMPGTVADYFTRLRELISDLPAALE
                     RRASHDATSARAQPDADSVPEYYAQDFHFQAGGYLSDGSARLYDLQVETLFYGSAAAM
                     RRAGLAPIAHFVKGRDQRKLALLDVACGTGRFLRQVRLAFPALRLTGLDLSRPYLHEA
                     ERHMQGLRPAKLIAANAESIPLADASQDIVSCIYLFHELPPEVRRRVTAEIARVLKPG
                     GLLVFIDSLQMGDKPGWDGLLEGFPVRFHEPYFEHYASDDLDGTFAEAGLQEESTSLS
                     FLSKVMVRRKMAA"
     gene            2163..2651
                     /locus_tag="VF226_06505"
     CDS             2163..2651
                     /locus_tag="VF226_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX7073677.1"
                     /translation="MSDNGSTLKPAAQRVAVAARALGLDIEVRTMSQSTRTAEEAAAA
                     CGCAVGQIVKSLVFRGKETGKPYLLLVSGSNRVNEKAVGKIIGEALQRPDAHYVRDIT
                     GFAIGGIPPIGHASRLTTYMDGDLLQYEVVWAAAGTPESVFPVAPADLRDKAGAEVIA
                     VN"
     gene            complement(2728..3444)
                     /locus_tag="VF226_06510"
     CDS             complement(2728..3444)
                     /locus_tag="VF226_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX7073678.1"
                     /translation="MLDKRNSVVAITAAILAFSASGGAFAGDRYGNFMVRVEGRVVDP
                     DSSADVFAAGVLQPTFDADISTAVVPTLTLTYFFTPNIALELLCCAAKFEAEGRKAIN
                     GVDLGDFWSIPPALTLQYHFDHWGGFKPYIGAGIHYMYFFNEGRSDFVAPLNGARLKL
                     DDALGFTVQAGVDIEIANGWYLNADIKKTWIDTDASWEGTPVTAEVDIDPWIYTVGIG
                     YRFNLEDIFGRRTAEYVPLK"
     gene            complement(3729..4436)
                     /locus_tag="VF226_06515"
     CDS             complement(3729..4436)
                     /locus_tag="VF226_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX7073679.1"
                     /translation="MMLGRKSLAAVALAAGMALATVSTAFAGGKDGNFMVRIQGTVID
                     PDASANVYSGGRLAPTYDAEVNTEVLPTMTLTYFFNKNLAVELLCCFAKLEAEGKRGA
                     IDGVDLGDFWIFPPALTLQYHFDNFNGFKPYVGAGIQYIHFFSEGRSDLGGRIEFDDA
                     FGFTLQAGVDVELGDGWYLNADVKKTWIDTDASWKGTDFTAKVDVDPWIYSVGIGYRF
                     NLEDIFGGRSAEHAALK"
     gene            complement(4717..>4906)
                     /locus_tag="VF226_06520"
     CDS             complement(4717..>4906)
                     /locus_tag="VF226_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX7073680.1"
                     /translation="AANIAFNMLKVVSGAVVLGPMLLGAEKAVHIVTPSITVRGLLNM
                     SAIAMVDSFRSDGAHQRE"
BASE COUNT          921 a         1525 c         1522 g          938 t
ORIGIN      
        1 gtgccgaccg tagtgatggt ttcatgcaac ccggcgactg cggggcgaga tgcgcgcatt
       61 ctgatcgatg gtggctaccg gctcgagtcc ctcacgccca tcgaccagtt tctctactcg
      121 gcacacctgg agctggtggc cgtcttccgg cgctgaccgc tattccgcct gggccaatag
      181 ccgatcgacc cattccggca cgatccgagt cgccggcccg tggatcgcct cggcgaacag
      241 gctgacgcct tccgacggtt cgaggttcag ctctacggta tgcgcgccag ttgcgcgggc
      301 ctcgcgcacg aacccggccg cggggtacac cgtgccgctc gtgccgatcg acgcgaacaa
      361 atcggcctcg gcgatgagct cgtagatctc gtccatgcgg tagggcatct cgccgaacca
      421 gaccacgtcg gggcgcatgc cgccgggttt cccgcaccca gggcacacca catcgaccga
      481 caggtcatcg ctccacggat ggcgcgcctc gcagcgcgcg caaagcgcct tcaacagctc
      541 gccgtgcata tgaatgagcc gccgcgagcc tgcggcttca tgcagcgcat cgatgttctg
      601 cgtgacgatg gtgaccacgc cgggatactc ccgctccagc cgggccagcg cccgatgagc
      661 ggcattcggc gcgacgtccc gaagcccgcg ccgccgttga ttgtagaagt cgtggacttt
      721 ggcgggatcg cgggcgaagg cctcgggcgt cgccacttcc gtcaggtcgt acttgctcca
      781 gatgccgtcc ttgtcgcgga aggtggcgag ccccgactcg gcggacaggc cggctccggt
      841 gagaatgacg atgcgctcgt atcgggccat gcaagcctct ttcgtgcacg cgactgaaaa
      901 tgtccacgta gggaagttcc tggcgctagg caaatggttc aaggtggagc aggcactggt
      961 ccgattgcgg ccgcggacgc gtgttacaac atcagatccg atcggcaacg agggaggcga
     1021 agatgagcgg cgcgcttggc atgtcggcac aactcgccag caacgcgatc cgcttcggct
     1081 ggtactacgg cctcaatcag ctgctcgacc gccgggcacg gcggctcagc gcgcgcccga
     1141 gctatcggcc cgagcgcccg gtgccgacgc ggcaagagct tctggccgat ctcggccggc
     1201 tgttcctaga ggatgcggcc cgtgtccgcg acggaatcta tccggccggc gaagagatgc
     1261 cgggcacggt ggccgactac ttcactcgcc ttcgggagct gataagcgat cttcccgccg
     1321 cattggagcg gcgggcgagc cacgacgcca cgagcgcccg cgcccagccg gacgccgata
     1381 gcgtgccgga gtactacgcg caggatttcc acttccaggc gggtggctac ctcagtgatg
     1441 ggtccgcccg gctctatgat ctccaggtcg aaacgctgtt ctacggcagc gccgccgcga
     1501 tgcgccgggc gggcctggcg ccgatcgctc atttcgtgaa agggcgcgat cagcggaagc
     1561 tggccctgct cgacgtggcc tgcggcacgg ggcgttttct gcgtcaggtc cgcttggcgt
     1621 ttccggccct gaggctgacc gggctcgacc tgtcccggcc ctatcttcac gaggcggaac
     1681 ggcatatgca gggccttcgt cccgcaaagc tgatcgccgc caatgccgag tctatcccac
     1741 ttgccgatgc cagccaggat atcgtcagct gcatctatct cttccatgaa ctgccgcccg
     1801 aggtgcgccg gcgggtaacg gctgagatcg cacgcgtcct gaagcctggc ggactgctcg
     1861 tgttcatcga cagtctccag atgggcgaca agccaggctg ggatggcctg ctggaaggat
     1921 ttcccgtccg cttccacgag ccctatttcg agcactacgc cagcgatgac ctggatggca
     1981 cgttcgccga ggcggggttg caggaggaaa gcacctcgct ttcctttctc tccaaggtga
     2041 tggttcgtcg gaagatggca gcctgaggcc gtcggctccg gcagcgccgc aacacgctca
     2101 tgctttgcgc ttccgcgtgc ccggtataat gctgtgctgc ttgcaatttc agggggatgt
     2161 cagtgtcaga caacggcagt actttgaagc ccgcggcaca gcgggtcgcg gtggcggcgc
     2221 gtgcgctcgg gctcgacatc gaggtgcgga ccatgagcca gtcgacgcgc acggccgagg
     2281 aagcggcagc ggcctgcggc tgcgcggtgg gccagatcgt caagtcgctg gtgttccgtg
     2341 ggaaagaaac cggcaagccc tatctgctgc tggtctccgg gtccaatcgc gtgaacgaga
     2401 aggccgtcgg caagatcatc ggcgaagcac tgcagcgccc ggatgcccat tacgtgcggg
     2461 acatcacggg ctttgccatc ggcggtattc cgccgatcgg acatgcaagc cgcctcacga
     2521 cctacatgga tggcgatctt ctccagtacg aggttgtctg ggcggctgca ggtacgccgg
     2581 aatccgtatt tccagtcgcg ccggcagacc tcagggacaa ggcgggcgca gaggtcatcg
     2641 cagtcaactg acaggagagc attggctgca ataaatgagt gtgcagcgga acacgaaggc
     2701 cgcagcgcag ggctccgggg acaccggtca cttcagcgga acatactcgg ccgtgcgccg
     2761 cccgaagata tcttccaggt tgaagcggta gccgatcccg acggtgtaga tccagggatc
     2821 gatatcgact tcggcggtga cgggggtccc ctcccaggag gcatcggtat cgatccaggt
     2881 cttcttgatg tcggcgttga gataccagcc gttggcgatc tcgatgtcga cacccgcctg
     2941 cacagtgaac cctaaggcgt catcaagctt gagccgcgct ccgttgagtg gcgcaacgaa
     3001 atcgctcctg ccctcgttga agaagtacat gtagtgaatg ccggccccga tatacggctt
     3061 gaagccaccc cagtggtcga agtgatattg gagcgtcaaa gctggcggaa tgctccagaa
     3121 atcgccgaga tcgaccccgt tgatggcctt tctgccctcc gcctcgaact tcgcggcaca
     3181 gcagagcaac tccagcgcga tgtttggagt gaaaaagtag gtcaaagtca gggtcggaac
     3241 gacggccgtg ctgatatctg catcaaaggt tggctgcagg acacccgcag caaagacatc
     3301 ggcgctcgag tcgggatcca ccaccctgcc ttccacgcgg accatgaagt ttccatacct
     3361 atcgcccgcg aacgcgcctc cagatgcgga gaatgcgaga atggcggcgg ttatggccac
     3421 aacggagttt cttttgtcga gcattatgcg cctccagttg atgctggcgc catctacgct
     3481 ggcccaactc agcgaatcgt ttacctacaa ctgtaggttg caatcgcgca aaagaggaat
     3541 ggcttcaaga agtgaagacc cttgtttgag ctagtcacgt cgcttgttgc aggacggcaa
     3601 cgcaattttg ggttgcgcca ttggtgacga tgtttcggct gaagacaatt cagcgcgcgc
     3661 cgactttcga gtggtcgggc gcgcgccgtg atgaaaacac gtaagtggcg cacgtctgtc
     3721 cggcgccgtc acttcaatgc agcatgctcc gcagaacgac cgccaaagat gtcctccagg
     3781 ttgaaccggt agccgatgcc cacgctgtag atccacgggt cgacgtcgac tttggcggtg
     3841 aagtccgttc ccttccagga ggcatcggtg tcgatccaag tcttcttgac gtcggcattg
     3901 agataccagc catcaccaag ctcgacatcg acgcctgcct gcagtgtgaa cccgaaggcg
     3961 tcatcgaact cgattctgcc acccagatcg cttctgccct cgctgaaaaa gtggatatat
     4021 tgaatacccg ccccgacata gggcttgaag ccgttgaagt tgtcgaagtg gtactgcagc
     4081 gtcagcgccg gcgggaagat ccagaagtcg cccagatcga cgccgtcgat agctcctctc
     4141 ttgccttccg cctccagctt cgcgaagcag cacaggagct ccacggcaag gttcttgttg
     4201 aagaagtagg tgagggtcat cgtcgggagg acctcggtgt tgacctcggc gtcataggtc
     4261 ggcgccaacc tgccccccga atagacgttg gcactcgcat ccgggtcgat caccgttccc
     4321 tggatgcgga ccatgaagtt gccgtccttc cccccggcaa acgccgtgct gaccgtggcc
     4381 agcgccatgc cggctgcaag tgccaccgcg gccaagcttt ttctgccaag catcatccca
     4441 tctccgtcag tcggggctgc agtcgctgat tccccctgaa tcagcagaca ccccatttcg
     4501 ggaacctgag cggaaaagtg atgcgtctca acgagctagg tggtcgcagt taccgagatg
     4561 tgccagcttt gcgcgatgat catcgatgct tgatgcagat cagagcaact ccgacgaatc
     4621 tctgtattaa ttacaagctg agcccctaat gcagatcgtc cgaatactgc atttctgctg
     4681 cgcgcgcggc gtttctcctg atcaatagtt tgccgtctat tcgcgttggt gagctccatc
     4741 cgatctgaag ctgtcgacca tggcgatggc gctcatgttg agcaggccac ggacggtgat
     4801 cgacggcgtc acgatatgca cggccttttc cgctccgagg agcatcgggc cgaggacgac
     4861 cgctccggac accaccttca gcatgttgaa ggcgatgttg gcggcg
//
LOCUS       DASVDU010000103         6412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_423930, whole genome shotgun sequence.
ACCESSION   DASVDU010000103 DASVDU010000000
VERSION     DASVDU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423930"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VF226_06525"
     CDS             complement(<1..346)
                     /locus_tag="VF226_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX7073681.1"
                     /translation="MRGKKNVDRYSSCLYAIGMSAEEDFEIFLVSAPGLESVLCEEAR
                     ANGFAEATPVKGGVTIRGGWPEVWRANLEIRGASRILVRIGSFRALHLAQLDKRARRL
                     PWGELLRRDVPFR"
     gene            780..2720
                     /locus_tag="VF226_06530"
     CDS             780..2720
                     /locus_tag="VF226_06530"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7073682.1"
                     /translation="MMTERIASLKHHLASLVAAVPRLPDEFRTAGSRLVDELQGRSTA
                     EVLVLILGFLALGAGTEWLFGMALRSVGKPMAALPRKTTSGRLRTIGLRFALDLGGLV
                     IFTLGSLGAFLAFNWPPILSQIVVAYLVAVVVLRLALVIGRVLLAPRCEPPSQADYFR
                     LIPMSTERAQYWCRRLALFVGWFALGWATVDVLRALDFTPAARQVVAYTLGIGLLAIS
                     MEAAWTGLRSESGAREDVHARTSHIIRASLLSVCFVLLWACWVSGFSRLFWLGVVALL
                     LPPAIRLSERTVRNALRPPGDASVATDMDLTTVYVERGVRALLIVGAVLLLAWAWQID
                     LVEMTSRDTLPTRLARGVLSSIVIILVADLVWQVTRTVIDGRLARLTEMAPPGSEEVL
                     RQARLRTLLPIFRTAMLILLVVLATLMALSALGVEVGPLIAGAGIVGVAIGFGSQTLV
                     RDVISGIFYLLDDAFRVGEYIQSGRYKGTVETLGIRSVKLRHHRGPVYTVPYGQLGAV
                     ENMSRDWVIDKMTINVTYDTDLDKAKKIVKQIGKELAQDPEYAPHLIEPLKMQGVEQF
                     GDFAIQLRLKMMTRPGEQFAIRRKAFAMLKRAFDENGIKFAFPTVQVAGRDEATAAAA
                     QQAIELTKTAPGTVSERADGVP"
     gene            complement(2757..3323)
                     /locus_tag="VF226_06535"
     CDS             complement(2757..3323)
                     /locus_tag="VF226_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016555863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073683.1"
                     /translation="MAMTPISNLVRHEGDPHVIRSGPSSLGMPDQLARMLGWFSIGLG
                     LAQIFMPGRLTRWLGTEGSENLVRACGAREIGAGLLCLSIDKELGLWSRAAGDGLDLA
                     TLLPAMRESNPQSGNAGMAAALVTGIMLLDVLAAQLIRGRHSEGNGRRRDYRDRSGFP
                     KGIEAARLAVKSSKQNVPPLTTTHSPAS"
     gene            complement(3338..4513)
                     /locus_tag="VF226_06540"
     CDS             complement(3338..4513)
                     /locus_tag="VF226_06540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX7073684.1"
                     /translation="MKALVWHGKEDIRYDTVSDPEIQDPRDAIVKVTSCAICGSDLHL
                     FHNLIPAMMPGDIMGHETMGEVVEVGSEMDGRLRKGERVVIPFTIICGECEQCKRGNF
                     SVCERTNRNKHLAEKIFGHTTAGLFGYTHITGGYPGGQAEYVRVPFADTTHVKVPESL
                     SDEQALFLSDILPTGWQAAVHCDIEPTDTVAIWGCGPVGQMAIRSAILLGAKQVVAID
                     RLSDRLSMAEAGGAIPINFDQESVIGRLFELTGGKGPEKCIDCIGMESHVSLSQPDTV
                     YDRAKQMLMMENDRPHVLREMIYVCRPAGIISIPGVYSGFSDKIPMGAAMNKGLTFRM
                     GQTHVHRWTDDLLKRIEEGQIDPSFVITHSVGLEHGPEMYKVFRDKQDSCIKVVLKP"
     gene            4726..5100
                     /gene="arsC"
                     /locus_tag="VF226_06545"
     CDS             4726..5100
                     /gene="arsC"
                     /locus_tag="VF226_06545"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578665.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEX7073685.1"
                     /translation="MAVIIYHNPSCGTSRKTLAMIRQSGEEPEVIEYLKTPPSRERLV
                     ELIRKMGITPRALLRRRGTPYDELGLDDPKWTDDQIIDFMITYPILINRPIVVTPKGV
                     RLCRPPETVLEILPNPNIGQSS"
     gene            complement(5175..>6412)
                     /locus_tag="VF226_06550"
     CDS             complement(5175..>6412)
                     /locus_tag="VF226_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7073686.1"
                     /translation="EARLRRETEDTLRQSQKMEAIGQLTGGVAHDFNNLLTVILGNLD
                     TVHRRLSNVAAGQEAAHFATTLLKPIELAIHGSRSAAQLTHRLLAFSRRQALEPTHLD
                     LNRLVAGMSDLLHRTVGESIKVETVLAGGLWPTFADANQVENVLLNLVINARDAMPAG
                     GNLTIETANTYLDETYARRFGDVAPGQYVMLSITDTGTGIPRDMLDRVFEPFFTTKGT
                     GVGSGLGLAMVHGFVKQSHGHVRIYSEVGVGTTVKVYLPRSTETADMPANPVGTEGPA
                     LPVPRAQRNETVLVVEDNANVREYAVSVLEDLGYRVIEAADVDGALKAIEDAPPIDLL
                     FTDVVLPGKESGRDLANILKQRFPGLPVLFTTGYTRNAIVHNGRLDAKVHLLNKPYTQ
                     QELARKLRELLDAAKASGN"
BASE COUNT         1152 a         1943 c         1991 g         1326 t
ORIGIN      
        1 cccggaacgg gacatcccgg cgcaggagct ctccccaggg caaccggcgc gcgcgcttgt
       61 cgagctgggc gagatggagc gcccggaaac tgccgatgcg gacgagaatc cggctcgcgc
      121 cacggatctc gagattggca cgccagacct ccggccagcc acctctgatc gtcacgccgc
      181 ccttgaccgg ggtggcctca gcgaagccat tggcccgcgc ctcctcgcaa agcacggatt
      241 ccagccccgg cgcggagacg agaaaaattt cgaagtcctc ttctgcgctc atgccgatgg
      301 catacaggca agaggagtac cgatcgacat tcttcttgcc gcgcattgct tggcggcttt
      361 cacggaacaa acctgtcgac cgctggttgt ttgttcgact gccgcacctt tccacgcatt
      421 gaatgctcgt gcgctggtcc gccgtgacct ttggggcgca tcgccaatcg cgagctgcgc
      481 cggcacattt gtccaaggag ccgcacttgc gggcgttggt gatggcgaga gcctcaggcg
      541 ccgccttgcg gagggctctc cgatgggtgc cctccatatt ggcgctgctc gctttgagcc
      601 ttgctggatg tttttccccg gcctatggcc aaccgcaacc cccgcagggt gcgcctccag
      661 cacaggtcca acaactcatg caactgctgc aggacccggc ggtacgagcc tggattgagc
      721 agcagccgaa aagtggatcg cctcccgccg aattggctac gccccaaatg acatccggaa
      781 tgatgaccga gcggatcgcg agcctgaagc atcatctcgc gtcgctggtt gccgctgtac
      841 cccgcctgcc agacgagttc cgcactgccg gcagccgcct cgtcgatgag ctgcaggggc
      901 ggagcacagc tgaggtgttg gtcctgatcc tcgggttcct cgcccttgga gccggaacgg
      961 aatggctctt tggaatggcc ttgcgttctg tcggcaagcc gatggcggcc ttgccgcgga
     1021 agacgacctc agggcggctg cggacaatag ggttacgctt cgcgctcgat ctcggcggtc
     1081 ttgttatttt caccctcggg agcctggggg cctttctcgc attcaactgg ccgccgatcc
     1141 tcagccagat tgtggttgcg tatctcgttg ctgtcgtcgt actgcggctc gctctggtca
     1201 ttggccgagt cctgcttgct ccgcgttgtg agcctccatc gcaagccgac tatttccggc
     1261 tgatcccgat gagtaccgag cgggcgcaat actggtgccg tcgcttggcc ctgttcgtgg
     1321 gctggtttgc ccttggatgg gcgacggtcg acgtgctgcg ggccttggat ttcacgccgg
     1381 cagctcgtca ggtcgttgct tacaccctgg gaattgggct gctcgccatc agcatggaag
     1441 ccgcatggac agggctgcgt tcggaatcag gagcgcggga ggatgtgcac gcccgaactt
     1501 cgcatatcat ccgcgccagt ctgctgtccg tctgcttcgt cctgctctgg gcgtgctggg
     1561 tttcaggatt ttcgcgcctg ttctggctcg gggtggtggc tctgctgctt ccgccagcga
     1621 tccggctgag cgagcggact gtccggaatg ccttgcgtcc gcccggtgac gcaagcgttg
     1681 cgacagatat ggatttaacc acggtttacg tggaacgagg cgtccgcgcg cttctgatcg
     1741 tcggagccgt tctcttgctg gcgtgggcct ggcagatcga tctcgtcgag atgacgagcc
     1801 gcgatacgct tcccactcgc cttgccagag gcgtcctgag cagcatcgtg atcattctgg
     1861 ttgcggacct cgtatggcag gtaacgagga cggtgatcga tgggcgattg gcaagattga
     1921 cagagatggc gcctccagga agcgaggagg tgctgcggca ggctaggctg agaactctcc
     1981 tgccgatctt ccgcaccgcc atgctcattc tgctggtcgt cctggccacg ctgatggccc
     2041 tctccgctct gggagtagaa gtcgggccgc tgattgccgg cgccggaatc gttggggtcg
     2101 cgattgggtt cggctcacaa acactggtga gggacgtcat cagcggcata ttctatctgc
     2161 tcgacgatgc tttccgcgtt ggcgaataca ttcagagcgg caggtataaa gggaccgttg
     2221 aaacgctggg catccgatcc gtgaaacttc ggcaccaccg tggccccgtc tacacggtgc
     2281 cttacgggca attgggtgca gtggagaaca tgagccgcga ctgggtgatc gataagatga
     2341 cgatcaatgt gacttacgat accgacctcg acaaagcaaa aaagatcgtg aagcagattg
     2401 gaaaagaact cgcgcaggac cccgagtacg ctccccatct gatcgagcca ctcaagatgc
     2461 agggcgtgga gcaattcggt gatttcgcaa ttcagctacg cttgaagatg atgaccagac
     2521 caggcgaaca attcgccatt cgccgtaagg cgttcgcgat gctcaaaaga gctttcgacg
     2581 agaacggcat caaattcgca ttcccgaccg tccaggtggc gggccgtgac gaggcgacgg
     2641 cggccgctgc tcagcaagcg atcgagctga ccaagactgc accgggaact gtttcagagc
     2701 gagcagatgg tgtgccttga gatcgaggtg cacgcgtctg gatcattttg acaaaattaa
     2761 cttgctgggg aatgcgtcgt cgttagcgga ggaacatttt gtttcgagga tttgaccgcg
     2821 agccgggcag cctcgatgcc tttcgggaaa ccgctgcgat cgcgataatc gcgccgcctg
     2881 ccgtttccct cgctatgccg tccccggatc aactgcgccg cgagcacgtc gagcagcatt
     2941 atcccggtga ccagcgcggc ggccattccg gcatttccgc tctgggggtt gctttcgcgc
     3001 attgcgggca acagcgtcgc gaggtccagg ccatcgccgg ccgcccggct ccatagcccg
     3061 agctccttgt cgatcgacag gcacaatagt ccggcgccga tctcgcgcgc accacaggcg
     3121 cgtacgaggt tttcagagcc ttcggtgccg agccagcggg tgaggcgacc tggcatgaaa
     3181 atttgcgcga gtcccagccc gatgctgaac caccccagca tgcgggcgag ctgatccggc
     3241 atgccgagtg agctcgggcc ggaccgtatc acgtgcggat cgccttcatg gcgcacgaga
     3301 ttggaaatgg gtgtcattgc cattgtgggc ctcgtcttca gggcttgaga acgaccttga
     3361 tgcagctgtc ctgcttgtcg cggaagacct tgtacatctc gggcccatgc tcgagcccga
     3421 ccgagtgcgt gatgacgaac gacggatcga tctgcccctc ctcgatccgc ttgaggaggt
     3481 catccgtcca gcggtgcacg tgcgtttgac ccatgcggaa ggtcaggcct ttgttcatgg
     3541 ctgcacccat cgggatcttg tccgaaaagc cgctgtaaac gcccgggatc gagatgatcc
     3601 cggctgggcg gcagacgtag atcatctcgc gcagaacatg cgggcgatcg ttctccatca
     3661 tgagcatctg cttggcgcgg tcgtaaaccg tgtctggctg cgagagcgaa acgtgacttt
     3721 ccattccaat gcagtcgatg catttttccg gccccttgcc gcccgtgagt tcgaaaaggc
     3781 ggccgatgac gctttcctgg tcgaagttga tggggatggc gcctccggcc tcggccatgc
     3841 tgagccggtc ggatagccgg tcgatggcaa cgacctgttt cgcaccgagc agaatcgcgc
     3901 tgcggattgc catttgtccc acaggtccgc agccccagat cgcgacggtg tcggtcggct
     3961 cgatgtcgca atgcacggct gcctgccaac ccgtcggcag gatgtcgctc aggaatagcg
     4021 cctgctcgtc agacaggctt tccggcactt tgacgtgggt ggtgtccgca aagggcacgc
     4081 gcacgtattc cgcctgcccg cccgggtagc cgccggtgat atgtgtatag ccgaacaggc
     4141 ccgccgtcgt atgtccgaag attttttcag ccagatgctt gttgcggttc gtccgttcgc
     4201 agactgagaa attcccgcgc ttgcactgct cgcattcgcc gcagatgatc gtgaacggaa
     4261 tgacgacccg ctcgcccttg cgcagccgcc cgtccatttc cgagccaacc tcgacgactt
     4321 cacccatggt ctcgtgaccc atgatgtcgc cgggcatcat cgcggggatg agattgtgga
     4381 agaggtggag gtcggagccg caaatcgcac agctcgtcac tttcacgatg gcgtcgcgcg
     4441 gatcttgtat ttcgggatcc gaaaccgtgt catacctgat gtcctccttg ccgtgccaaa
     4501 cgagggcttt catccgtcac tccttgcatc aggcattgag ccgcgatcgt tgcggaccct
     4561 caagcaggcg agcgcatgcg gccggctcac gggttgtgcc gtgataagcg ccagccatcg
     4621 ggaagcgttc cccgcggccc ccgtgccaga tcacgggcgc atcattcgtg tgtagaatag
     4681 gagccgactg cgccgggcgg cagtcaagaa cggaggagcg gtgccatggc ggtcatcatc
     4741 tatcataacc cctcgtgcgg aacgtcccgc aagacgctgg ccatgatccg gcaatcggga
     4801 gaggagccgg aggtgatcga atatctcaag acgccacctt ctcgggagcg tctggtcgaa
     4861 ttgatccgca aaatgggcat cacgccccgc gcgctgctgc gtcggagagg aacgccttac
     4921 gacgagctcg gcctggatga tcccaaatgg accgacgatc agatcatcga tttcatgata
     4981 acgtacccga tcctgatcaa ccggcccatc gtggtgacac caaaaggcgt ccgactgtgc
     5041 cgcccgccag aaacggtgct ggaaattctg cccaatccca atatcggtca gtccagttaa
     5101 taggatggcg agtggtcaga aatcagaagg gctgaacatt ttcattctta gctgcatttc
     5161 tgcgggctcg cacttcagtt tccgctggcc ttggccgcgt cgagcagctc gcggagcttt
     5221 cgtgccagct cctgttgggt gtagggtttg ttcagcagat gcacttttgc atcgaggcgg
     5281 ccgttgtgga cgattgcgtt gcgggtatag ccggtggtga acagcacggg cagaccgggg
     5341 aagcgctgct tgagaatatt cgcaagatcg cgcccgctct ctttgcccgg cagaacgacg
     5401 tcggtgaaaa gcaagtcgat aggcggtgca tcttcgatcg ccttgagcgc gccatcgacg
     5461 tcggccgctt cgatgactcg gtagcccagg tcctcgagca ccgaaacggc atattcacgc
     5521 acgttcgcgt tgtcctcgac tacgagcacg gtttcgttgc gttgcgctcg cggcacggga
     5581 agcgccggac cttcggtgcc gaccgggttg gctggcatat ccgcagtctc cgtgctgcgc
     5641 ggaaggtaga cctttaccgt ggtccccacg ccaacctcgc tgtagatgcg gacgtggccg
     5701 tgcgactgct tcacgaagcc atgcaccatc gcgagaccaa ggcccgagcc gacgcccgtt
     5761 cccttggtcg tgaaaaacgg ctcgaaaacg cgatctagca tatcgcgtgg aattccggtt
     5821 cctgtatcgg ttatgctcaa catcacgtac tgcccgggcg cgacgtcccc gaagcgccgg
     5881 gcgtatgtct cgtccaggta cgtgttcgca gtctctatcg tcagattccc accggccggc
     5941 atggcatcgc gcgcattgat gacgaggttc agcaggacgt tctcgacctg gttggcgtcg
     6001 gcgaatgtcg gccacaatcc gcccgcgagc accgtctcca ccttgatgct ctcaccgacg
     6061 gtgcgatgga gcaggtcgga catgccggct acgagacgat tgagatcgag gtgcgtcggt
     6121 tcgagcgcct gcctgcgcga gaacgcaagc agtcggtgcg tcagttgagc cgcgctgcgg
     6181 ctgccgtgga ttgcgagctc gatgggtttc aggagcgtcg tcgcaaagtg ggcggcctcc
     6241 tgccccgcag ccacgttgct gagccggcgg tggaccgtgt cgagattgcc gagaatgact
     6301 gtcaacagat tattgaaatc gtgagccacc ccgcccgtga gctgcccgat ggcttccatc
     6361 ttttgagact ggcgcaacgt gtcctctgtc tcgcgcctca gccgcgcttc gc
//
LOCUS       DASVDU010000104         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_42579, whole genome shotgun sequence.
ACCESSION   DASVDU010000104 DASVDU010000000
VERSION     DASVDU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42579"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..832)
                     /locus_tag="VF226_06555"
     CDS             complement(302..832)
                     /locus_tag="VF226_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7073687.1"
                     /translation="MVYATATIRSTPVFGLRREIDRLFEDTFGRGTVGTDAWTPAVDI
                     RENEKELMFTVDLPGVKPENVDVTAADGVLTIQGERLDDRTDEGAGQYYLAERNYGSF
                     LRRFQLPQGVDTEKILADVEHGMLQVHIPKASLPQPKKITVTSTSDVSPKAQLADANG
                     SRKEPVKLAAGMRQSH"
     gene            1048..1989
                     /locus_tag="VF226_06560"
     CDS             1048..1989
                     /locus_tag="VF226_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX7073688.1"
                     /translation="MRVVTRRTGLSADLLRAWERRYEVVKPSRSNGGHRIYSDADIER
                     LRLLYRATLGGRNIGQVADLPNTVLAELVRRDAAADGVAERAGEAAQRAIGDEWRVAR
                     DDLGECLRTAERLDATALEARLRHAAVVLPAAAFCDMLAVPLLERMGTRWREGTLRPV
                     HGHLVSVAMRRVLESLIRSATLPLATPNLVVATPAGQVHEFGALLVAMTAAAEGWRVT
                     YLGPGLPAEDIAEAAMRTRARAVALSVVYPARDPALTDEFRRLRTTLPKKVTILAGGA
                     ASSGYSGILDDIGAVRLDDLESFRVRLRTLGGARRPK"
     gene            complement(2048..2206)
                     /locus_tag="VF226_06565"
     CDS             complement(2048..2206)
                     /locus_tag="VF226_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073689.1"
                     /translation="MHIAEFNFFLVSDDESTWPTARVVRRRSFVAISAWVLFALHAIV
                     AYHLLNVH"
     gene            2256..>2474
                     /locus_tag="VF226_06570"
     CDS             2256..>2474
                     /locus_tag="VF226_06570"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7073690.1"
                     /translation="MGTTTAPDLARAAQLFQALSDDTRLDILGMLRDGERCVCELQDA
                     LDSAQSRLSFHLRVLKEAGLVTDRKEGRW"
BASE COUNT          424 a          799 c          805 g          446 t
ORIGIN      
        1 attgggacgc caccagatgt cgtcgtcggt aaggcgtccc acgcaggcgg tgatcttggg
       61 gagaaagtcg cgctgcagga agttgcgtga ctgttcgaga aatgcgtgtc ctgtatcggt
      121 catggtcagg tactcatgtt gccggagctg gatgctgatg acgggcgcca gcccggtcgg
      181 gcctgctgaa agatagagag aagccaggcg acaggcgaac ggcacaacgg cgcgggagcc
      241 ctgcccccgc gccgttgaac gagcctgcgc gtgctgtagc tggccactgc gtgaccggcc
      301 tctagtgcga ctggcgcatc ccggcggcta gtttcaccgg ctctttccga ctgccgttcg
      361 cgtcggcaag ctgcgccttc gggctcacat ccgacgttga tgtcaccgtg atcttcttcg
      421 gctgcggcag cgaggccttc gggatatgca cctgcagcat gccgtgctcg acatcggcca
      481 gaattttctc ggtgtccact ccctgcggca gctggaagcg gcgcaggaac gagccatagt
      541 tgcgctcggc caggtagtac tgccccgcgc cctcgtctgt ccggtcgtcg agccgctcac
      601 cctgaatcgt caggacccca tcggccgccg tcacatcgac gttctccggc ttgacgcctg
      661 gaaggtcgac cgtgaacatc agctcctttt cgttctcacg gatgtcgacc gcgggggtcc
      721 acgcgtccgt cccgaccgtg ccgcgtccga aggtgtcctc gaacagccgg tcgatttccc
      781 gccgcagacc aaagacgggc gtgctccgga tcgttgcagt tgcgtatacc atgatccatc
      841 tcgctttgca cagggagtcc tgccaacgca tgaccggaca gtcggactcc gacggcttcc
      901 agctcattta tccctgcatg cagtctagcc cgcggtcggt gagttgtcaa gctttcgtcc
      961 agaacgtaat gttgacaaca taatgcccgg ccgctagtct gggacgtcat gagaagcaag
     1021 gccaggcccg cacagcagtg gcatcccatg cgcgtggtca cccgacgcac ggggttgagc
     1081 gccgatctgc ttcgcgcatg ggagcgccgc tacgaagtgg tgaagccatc ccggtccaac
     1141 gggggtcacc gcatctattc cgacgccgac atcgagcgcc tccgcctgct ctatcgcgcc
     1201 acactcggcg gtcgcaatat cggacaggtg gcggacctgc ccaacacggt actcgcggaa
     1261 ctcgtgcggc gggatgcggc ggcggacggg gtggccgaac gcgcaggcga ggcggcgcaa
     1321 cgcgcgatcg gcgatgaatg gcgcgtggca cgcgatgatc tgggcgagtg ccttcgcacg
     1381 gcggaacggc tcgatgcgac ggcgctcgaa gcgaggctgc gccacgcagc ggtcgtactg
     1441 cccgcggccg cattctgcga catgctcgcg gttcccctgc tcgagcgcat gggcacgcgc
     1501 tggcgcgagg gaacgttgcg ccctgtgcat gggcacctcg tctcggtcgc gatgcggcgc
     1561 gtgctcgaga gcctgatccg gagcgcgacc ttgccgcttg cgacgccgaa tcttgtcgtc
     1621 gccacgccgg ccggccaggt ccacgaattc ggcgccctgc tcgtcgccat gactgcggct
     1681 gccgagggat ggcgggtgac gtatctgggc cccggcctgc cggccgagga catcgccgag
     1741 gcggccatgc gcacccgcgc acgcgcggtg gcgctgagcg tcgtctatcc ggcgcgggat
     1801 ccggcgctca ccgacgagtt tcgccgcctg cgaacgaccc tgccgaagaa agtcacgatt
     1861 ctcgcagggg gcgcggcatc gtcaggttat tccggaatac tcgacgacat tggcgccgtt
     1921 cgactcgacg atctcgaatc attccgggtg cggctcagga ccctcggtgg cgcgcggcgg
     1981 ccaaagtagc cattggaaat ccgaagacgc gacgcacgcc cgccggtggc ggctcacctg
     2041 tgtcagctca atggacattg agcaggtgat acgcgacgat cgcatgcaga gcgaacagca
     2101 cccatgcgct gattgccacg aacgacctgc gccggaccac tcgagccgtc ggccacgtgg
     2161 actcgtcgtc agagaccagg aagaagttga attcggcaat atgcatggcg agcgtggaat
     2221 tgacataaca acgatttttg atatattccc acaccatggg tacaacgaca gctccggacc
     2281 tggcacgcgc ggcacagctc tttcaggcgc tttccgatga cacccgtctc gacatcctcg
     2341 gcatgctccg ggatggcgag cggtgtgtct gcgagctcca ggatgcgctt gattccgccc
     2401 agtcgcggct ttcctttcac cttcgtgtgc tcaaggaggc cggcctcgtg acggaccgga
     2461 aggagggacg ctgg
//
LOCUS       DASVDU010000105        12978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1573043, whole genome shotgun sequence.
ACCESSION   DASVDU010000105 DASVDU010000000
VERSION     DASVDU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12978
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573043"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VF226_06575"
     CDS             complement(<1..472)
                     /locus_tag="VF226_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7073691.1"
                     /translation="MTAGASTTTDGRSIAVSAEKSYEEKAFGFWIYLMSDAIIFALLF
                     ATYIVMLGNTAGGPTGKELFSLERAAGETALLLLSSTTFGFASVALATSDRPRVLQWL
                     AVTAALGAGFLILELGEFSGMISQGAGPDRSGFLSAFFTLVGTHGLHVSIGILWI"
     gene            complement(469..2502)
                     /locus_tag="VF226_06580"
     CDS             complement(469..2502)
                     /locus_tag="VF226_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7073692.1"
                     /translation="MFGRLTIEAFPFYSAIAATGAAITVLGAVSVIAVITYFRAWRLV
                     LFDWISSLDHKKIGIMYVMLALVMLMRGFIDAAMMRSQQAIASGNDGYLPPGHFDQIF
                     SSHGTIMIFFMAMPFLSGLINHIMPQQIGARDVAFPFMNAISLWLTAAGAGLVLISLA
                     IGKFSTAGWSGYPPYSGAEYNPGVGVDYWLWAVLIAGVGSTMSGINFLVTILKCRAPG
                     MHLFRMPLFTWTVLVTSILMIFAFPALTVACGLLALDRIFGMHFFTNAMGGNMMHYIN
                     LFWLWGHPEVYILILPAFGVFSEVVATFSQKRLFGYISLVLATMAIGILSFSVWLHHF
                     FTMGASADVNSFFGIMTTIIAVPTGVKVFDWLLTMYRGRIIFKPPMLYTLGFLVTFVI
                     GGLSGVLLALPPVDYTMHNTTFLVAHFHNMIIPGVLFGYLAGYMFWFPKAFGFKLNEK
                     WGTRSFWCWIVGFYLAFMPLYVLGLMGMPRRMEHYDIPAWQPHLIVAAIGAFIILLGI
                     ICLGIQLAVSIRDREQARDLTGDPWNGRSLEWATSSPPAPYNFAILPHVQSIDAFWDM
                     KHRGVAYQRPDRYVDIFLPKNTFVGAFIGGAGFVFGFGMVWYIWWLAILALAAIALAI
                     IVRAFDDDTDTIIPASKVKEIEDARFAQLAKAPRNELADDPGFAAEAVPEGSS"
     gene            complement(2515..3369)
                     /gene="cyoA"
                     /locus_tag="VF226_06585"
     CDS             complement(2515..3369)
                     /gene="cyoA"
                     /locus_tag="VF226_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HEX7073693.1"
                     /translation="MLLTTDCGALADPVLDPKGPIALAQRQLLFDAVAIMMIVIIPVF
                     IMAALFIWRYRGTNRNARYTPNLAYYWPAEILIWGVPAGIILWLSFHLWDGTHKLDPY
                     KAVDPSVAPLQVEAVAQDWKWLFIYPEQNIAVVNELVFPSGRPLAIRITSDTVMNSFI
                     IPGLGGQIYAMAGMQTKLHLLADEPGEFWGRNVQYSGRGFADQQFRAIATSTGDDFDA
                     WIAKVKTSLDTLDATTYEQLAKPSVKVPVTYYSAVEPNLFDKIIAKWRDGGTAHHAPM
                     TAEAEQAN"
     gene            complement(3566..4894)
                     /locus_tag="VF226_06590"
     CDS             complement(3566..4894)
                     /locus_tag="VF226_06590"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7073694.1"
                     /translation="MTDSSATRLARVVIIGAGFGGLSAAKALSRAAFEVTLIDRYNYH
                     QFQPLLYQVATAALPPSVVASPIRAEFRRKPNVSVMLASVSAIDSDRNEVIADGHRVP
                     YDYLVVATGAKQSYFGHGDWAPDAPGLKSIDDAIYLRNRILLAFEKAEIESDPDERRR
                     LLNFVIVGGGATGVELAGAVAELARRTLRSDFRSIDPRSARVVLVEAGPRILHQFDES
                     LSRYAHRALEGLGAEVRTGTRVTGCDALGISMGNDRIESRTVIWAAGVEASPAGRWLG
                     AETDRAGRIKVEADLSLPGRPHIFAIGDTALCVDANRRPLPGVAPVAKQQGAYVARAI
                     SARAAGKPISPFRYKDYGMLATIGRSRAVAELPRVKMSGIAAWLVWCFAHIFFLIGFR
                     NRLIVMLDWAWNFTTSQLAARLITGNVGGQENSMHDSSRSEAKAVELKRP"
     gene            5138..6976
                     /locus_tag="VF226_06595"
     CDS             5138..6976
                     /locus_tag="VF226_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7073695.1"
                     /translation="MAHPIEDYALIGDCETAALVARDGSIDWLSWPRFDSDACFAALL
                     GNPEHGRWRIAPMDPGAQVTRRYHRDTLVLETEFSTRDGAVTLIDFMPPRSPLAGDTR
                     PEPGKASDVIRIVRGKRGQLAMSCELILRFGYGQIVPWVTRLGDGSGGLCAVAGPDMT
                     VLRTPIELRGDNFKIAGSFVVSEGETVPFVLSYGRSYRAPPEPTDPLAALSMTKELWR
                     DWLQGCREVGKWSDAVRRSLITLKALSYRPTGGIVAAPTTSLPEQLGGTRNWDYRYCW
                     LRDATFTLLSLMNGGFYQAARDWSQWLLRAVAGTPQQIQIMYAIDGERRLTELELPWL
                     PGYQGAKPVRIGNAASNQLQLDVYGEIMDAFYQGRRGGLDALDLDLQRALLDHLAQIW
                     RQPDEGIWEVRGGRQHFTYSKVMAWVAFDRAIKLGEEFAIEGPIDRWKAIRQAIHNEV
                     CEKAFNPGVGAFTQYYGADCLDASVLLMPLVGFLPASDPRVRGTVAAIERHLMVDGLV
                     MRYDTHGTKDGLPPGEGAFLACSFWLVDNLVMQGRMQEAERLFERLCGLCNDVGLLSE
                     EYDPSAKRLVGNFPQAFSHIALVNSAHNLVLSQNSLKQRAGHDRSA"
     gene            7117..8034
                     /locus_tag="VF226_06600"
     CDS             7117..8034
                     /locus_tag="VF226_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7073696.1"
                     /translation="MLGSDFQRPILIETNAHEGPVYVAAEHALYFTTVPEPGPKNIAI
                     RRVQLVGEEFPFEARPPETVRFPSNMANGMTLDREGRLLICEQGFKETPARISRMDLE
                     TRAVETVVDEWRGLQFNSPNDVVVKSDGTVWFTDPNYGELQGFKGTPKVGPYVYRHDP
                     GTGETAVVADNFNKPNGLAFSPDESVLYITDTGANQAPGTYFVGLPHHIRAFDVHGGR
                     HLRNDRLFAVISPGVPDGIKVDIQGRVYTSSATGVQVFTPDGDLLGEIMAPGVANFTF
                     GGPRNNVLFILGDTVIWQAQLQVSGNVTC"
     gene            complement(8229..9782)
                     /locus_tag="VF226_06605"
     CDS             complement(8229..9782)
                     /locus_tag="VF226_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7073697.1"
                     /translation="MDKHFDVVIIGSGAGGGTLARHLAPSGKSILILERGDWLKREAL
                     NWDATAVFRENRYVSPETWYDRNGRPFQPQVHYFVGGATKMFGAALYRLRKEDFGELR
                     HYDGISPAWPITYEEFEPYYTKAEQMYHVHGLRGQDPTEPPASAPYPCPPLSHEPRIQ
                     QLFDDIAAAGYHPFPAPAAIMLDESNMAYSTCIRCQTCDGFPCLVHAKSDAEVVAVRP
                     ALGYPNVMLLRNARALKLETNSAGTAVTSVVAEVEGVLDTFRGDIIVVSCGAANSAKL
                     LLMSANDKHPNGLANGSDQVGRNYMFHNSQAVLAISREPNPTMFQKTLALNDFYFGMD
                     GFDYPVGNIQMMGKSLGPMYRGEKPIETALLPMRALDEIAYHAVDFWLTTEDLPDPEN
                     RVTVDRSGKLILSYTPNNQVPAQKLYEKLKSMLNHLGMHPNHLIPRNAYMKTEIPIAG
                     CAHQAGTCRFGRDPQTSVLDVNCKAHELDNLYVVDTSFFVSIGAVNPSLTAIANAIRV
                     GDHLLQRLA"
     gene            complement(9798..10568)
                     /locus_tag="VF226_06610"
     CDS             complement(9798..10568)
                     /locus_tag="VF226_06610"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7073698.1"
                     /translation="MSLQNKVAIVTGGNSGIGQAIVLELARQGASGIVIDYVVHPEAT
                     AELEREIAKLGGQAIGVDADVSKIDDLQKLVDAAVAHFGRLDIMVNNAGIETRTSVLD
                     TTEDQYDRVLRINLKSAFFGTQIAAKQMIKQGDGGRIINISSVHEDWPMPGNTAYCLA
                     KGGMRMLTRTAGVELAPHNILVVGVGPGAVATPINLPTMEDPAKLQKLNSAIPLGRMA
                     RPEEIASVVAFLAGDGASYLTATSIFADGGLMQSSVGL"
     gene            complement(10678..10890)
                     /locus_tag="VF226_06615"
     CDS             complement(10678..10890)
                     /locus_tag="VF226_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073699.1"
                     /translation="MSTEKARHPFPRPPSPQPEHQATGRDYPAEWARRFELYLDKAPR
                     RAVFYGVLFAAFVIAVVISTIVKFGA"
     gene            11234..11941
                     /locus_tag="VF226_06620"
     CDS             11234..11941
                     /locus_tag="VF226_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073700.1"
                     /translation="MTPAAPLGAPQETGRGQRSTELAYLHIKQKILDNEYPPGAHVLE
                     QDIAAELKLSRTPVREALVRLQQDGLVKIVPRHGVRISPLSPADMREIYEVLMSLEPT
                     AVELLARRRPSLSELAPLVEACDAMEAALSRREPDLKAWAAADESFHINLARMCGNSR
                     LAAMIMTVWDQAHRARMFTLTLRPVPHRSTEEHRAVVEAILAGDAAGAHKLYAAHRRR
                     GGVELMSIIERHGLQHL"
     gene            11938..>12978
                     /locus_tag="VF226_06625"
     CDS             11938..>12978
                     /locus_tag="VF226_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX7073701.1"
                     /translation="MKSNVFHVVQMPGDGIGIEVMQAAGAVLAAVEARYGLGFRYETV
                     PGGAHYFKETGTVLPADGMDRAKRADAILFGAMGWPDIRFPDGTEIAPQLDLRFGLDL
                     YAGVRPIRAIPGVPTALSHPRAKEIDLVIVRESTEGMFASRGKGVVIDDREARDTQVI
                     TRRTTERLTDFCCRLARRRKAERGTPGRVTLVDKSNVFVSFAFMRQVFHEAAATHPDI
                     EARHHYVDAMALDLVRRPWDFDVLPMENLFGDILSDLAAGLIGGMGFAPSADIGDAHA
                     LFQPSHGSAPDIAGKGIANPTAMILSAAMMLDWLGERHGSDSARAAARAVEKSVDLVF
                     GSGRVRSCDIGGS"
BASE COUNT         2635 a         3859 c         3959 g         2525 t
ORIGIN      
        1 taatccagag aataccgatg ctcacgtgca gcccgtgagt gccgaccaag gtaaagaatg
       61 cggagagaaa gccgctccgg tccggacctg cgccctgcga gatcatacca ctgaactctc
      121 cgagctcgag aataaggaaa ccggccccga gcgccgcggt tacagccagc cattgcagca
      181 ctcgcggtcg atctgatgtg gcgagcgcga cagacgcgaa gccgaaagtc gtgctgctca
      241 gaagcaggag cgctgtctcg ccggcggcgc gttcgagact gaacagttcc tttccggtag
      301 ggccgccggc agtgttgcca agcatcacga tatacgttgc gaacagcagc gcgaaaatga
      361 tcgcgtcgct catcaagtag atccagaagc cgaacgcctt ctcctcgtaa cttttttctg
      421 cagaaacagc tatcgacctg ccgtcagtcg tagtggaagc gcccgcggtc atgacgagcc
      481 ctccgggacg gcttcggcgg caaaacccgg atcgtcggcc aattcgttgc gcggcgcctt
      541 tgccaattgg gcaaagcgcg cgtcctcgat ttccttcacc ttgcttgctg gaatgatggt
      601 gtcggtgtcg tcatcgaagg cgcggacaat gatggcgagt gcgatggcgg ccagggccag
      661 gatggcgagc caccagatat accagaccat accgaagccg aacacaaaac cagcgccgcc
      721 gatgaatgct cctacgaagg tgttcttcgg caggaagata tcgacatagc gatccgggcg
      781 ctgataggcg acgccgcggt gtttcatgtc ccaaaaggca tcgatgctct ggacatgcgg
      841 tagtatcgcg aaattataag gcgccggagg cgacgatgtc gcccattcga gcgatcggcc
      901 attccacggg tcgccggtca ggtcgcgtgc ctgctcgcgg tccctgatgc tgacggcgag
      961 ttggataccg aggcagatga tgccgaggag aatgatgaag gcaccgatgg ctgcgacgat
     1021 gaggtggggc tgccatgctg ggatatcgta atgctccatg cggcgcggca ttcccatcaa
     1081 tccaaggacg taaagcggca tgaacgccag atagaacccg acgatccagc accagaacga
     1141 ccgcgtgccc catttctcgt tcagcttgaa cccgaaggcc ttgggaaacc agaacatata
     1201 gcctgccaaa tagccgaaca gcacacccgg aatgatcatg ttgtgaaaat gcgcgaccag
     1261 aaaggtcgta ttgtgcatcg tgtagtcgac cggtggaaga gcgagcagca cgcccgaaag
     1321 gccgccgatg acgaaggtaa cgaggaaccc gagggtgtaa agcatcggtg gtttgaaaat
     1381 aatcctgccg cgatacatcg tcagcagcca atcgaagact ttcacgccgg tgggcacggc
     1441 gatgatggtg gtcataatgc cgaagaagga attgacgtcg gcgctggcgc ccatcgtaaa
     1501 gaagtgatgc agccatacgc tgaacgacag gataccaatg gccatggtgg ccagaaccag
     1561 cgaaatatat ccgaacagcc gcttctggct gaacgtggcg accacctcgg agaagacgcc
     1621 aaaggccggc aaaatgagga tatagacctc cggatggccc cacagccaga agaggttgat
     1681 gtagtgcatc atgttgccgc ccatggcatt ggtgaaaaag tgcatgccga atatgcggtc
     1741 gagcgcgagc aggccacaag cgacggtgag cgcgggaaag gcgaaaatca tcaagatgct
     1801 ggtaacgagc acggtccagg tgaagagggg catgcggaaa agatgcatgc ccggcgcgcg
     1861 gcacttgagg atggtgacga ggaagttgat gccgctcatg gtcgatccga caccggcgat
     1921 aagcaccgcc cagagccaat aatcgactcc cactccggga ttgtattcgg cgcctgaata
     1981 cggtggatag ccgctccagc ctgcggttga gaatttgcct atagcgagcg agataagcac
     2041 gagcccggcg ccagcggcag tcagccagag gcttatcgcg ttcatgaacg ggaacgcgac
     2101 atcgcgcgct ccaatttgct gcggcatgat gtgattgatg agtccggaga ggaacggcat
     2161 ggccatgaag aagatcatga tcgtgccgtg agacgagaag atctggtcga aatgcccggg
     2221 aggcaggtag ccgtcattgc ccgaggctat ggcttgctgg ctgcgcatca tggctgcatc
     2281 gatgaagccg cgcatcagca tcacgagcgc cagcatgacg tacatgatcc cgatcttctt
     2341 gtgatcgagg ctcgatatcc aatcgaacag gacgaggcgc catgcgcgga aatatgtgat
     2401 gacggcaatg accgaaacag caccaagcac ggtgatcgcc gctcccgtgg cagcgatcgc
     2461 gctgtagaac ggaaatgctt cgatcgttaa cctaccgaac acttccactt ttcctcaatt
     2521 tgcttgttcc gcttcggcgg tcattggagc gtgatgagcg gtgccgccat cacgccattt
     2581 cgcaatgatc ttgtcgaaca ggttcggctc gacagccgaa tagtaagtca ctggaacttt
     2641 cacgcttggc tttgcgagtt gctcataagt cgtcgcatcg agggtatcca acgaagtttt
     2701 taccttcgcg atccaggcat cgaaatcgtc tccggtgctg gtggcaatgg cgcgaaactg
     2761 ttggtcggca aaacctcgcc cactgtactg gacattgcgg ccccagaatt cgccgggctc
     2821 gtcggcgaga agatggagct tggtctgcat gccggccatg gcgtagatct gccctcccaa
     2881 ccccggaatg atgaacgagt tcatcaccgt gtccgacgta attctgatgg cgagcggcct
     2941 gcctgacgga aacacgagct cgttgacgac cgcgatgttc tgctcggggt agatgaagag
     3001 ccacttccag tcttgcgcca ccgcctcgac ctggagcggt gcgacgctgg gatcgaccgc
     3061 cttgtacgga tcgagcttgt gcgtgccatc ccacaggtga aagctgagcc aaaggattat
     3121 tcccgctggc acgccccaga tcaggatctc cgcgggccag taataggcga ggtttggcgt
     3181 atagcgcgca tttcggtttg tgccgcgata gcgccagatg aacagcgcag ccatgatgaa
     3241 gaccggtatg atcacgatca tcatgatggc cacggcgtcg aacaggagct gccgctgggc
     3301 gagtgcaatc ggacctttcg gatcgagaac tggatccgca agcgcgccgc agtcggttgt
     3361 gagcagcatc aggcatgagg ctgccgccac catttgcaat cgataggcga gagcttgtct
     3421 cacgatcagg ccttgcgatt cgaattctcg gtcgcgcgcg gggagcatcg gcatgcaaag
     3481 ccttgctccg gtgtctcgtc gcaatgcagg tggcgattgc tcagcctcac ccgccgttct
     3541 gctgcctaac gccgatatct gcgaatcacg gtcgtttcag ctcgacagcc tttgcctcag
     3601 atctcgagct atcgtgcatc gagttttcct gtccgccgac gtttccggtg atcagccgcg
     3661 ctgcgagctg ggatgtggtg aaattccacg cccaatccag catgacgatc agccggtttc
     3721 ggaaaccgat gagaaaaaag atatgcgcga agcaccagac cagccaagcg gcgatgccgg
     3781 acatcttcac ccgcgggagc tccgccactg cgcgactgcg tccgatggtg gcgagcattc
     3841 cataatcctt atagcgaaac ggcgaaatcg gtttgcccgc tgcgcgtgcc gagatggcgc
     3901 gcgccacgta ggcgccctgt tgtttggcaa ccggcgccac tcccgggagt ggccggcggt
     3961 tggcgtcgac acacaacgcc gtatccccga ttgcaaagat gtgcggccgg ccgggaagcg
     4021 aaagatcggc ttcgactttt attcgtcctg cgcgatcggt ctctgcaccg agccagcggc
     4081 ccgcagggga tgcttcgacc cctgctgccc agatgacggt acggctctcg atcctgtcat
     4141 tccccatgga aatgcccagc gcatcacaac ccgtcacccg ggttccggtg cgaacctctg
     4201 ctcccagccc ctcgagcgcg cggtgggcat agcgcgaaag cgactcgtcg aactgatgca
     4261 gaattcgcgg tccggcttcg accagaacga cccgcgccga gcgtgggtca atactgcgaa
     4321 aatcggaacg aagcgtccgt ctcgccaact cggccacagc cccagccagc tcgacaccgg
     4381 tggcaccgcc accgacgatc acgaaattca acagccggcg ccgctcatcc ggatcagatt
     4441 cgatctcggc tttctcgaag gcgagtagaa tccggtttcg caagtagatc gcatcgtcga
     4501 tagacttcag gcccggggcg tccggcgccc agtcgccgtg cccgaaatag ctctgctttg
     4561 ctccggtcgc gacaacaaga taatcgtatg gcactcggtg gccgtccgcg atcacctcgt
     4621 tcctgtcgct gtcgattgcg gaaacgctgg caagcatgac gctgacattc ggttttcggc
     4681 ggaactcggc ccggatcggc gatgcaacga ccgaaggcgg cagtgcagct gtcgcaactt
     4741 ggtagagcaa tggctggaac tgatgatagt tgtaacgatc gattaacgtt acttcaaacg
     4801 ccgcgcggct gagtgccttt gcggcggaaa gcccgccgaa ccccgctccg attatgacga
     4861 ctcgggcaag ccgggtcgca ctactgtccg tcatggggaa cctctctcgg agaggaccgt
     4921 ttggtgcgcg acattcaaca aaagtgttcg cggctatttc gttccactgc gcttgtttaa
     4981 ccaaggaagg tttggtggca agcttaagct atcgtgcttg gcacgcacgc gatatgattg
     5041 atgccacatc cgatctgacg gaataccgcg ctgcttgacc ccgcccatgt agcgcttgaa
     5101 cggtgtgatt gatttcgcag cgctataggc cgcagcaatg gcgcatccaa tcgaagatta
     5161 tgcccttatc ggcgattgcg agacggcagc gttggttgcc cgcgacggat cgatcgattg
     5221 gcttagctgg ccgagattcg atagcgacgc ctgctttgcc gcgttgctcg gcaatcccga
     5281 gcatggacga tggcgaatag cgccgatgga ccccggggcg caagtcacgc ggcgctacca
     5341 tagagatact ctggtcctcg aaactgaatt ctcgacgcgc gatggcgcgg tcacattgat
     5401 cgacttcatg ccacctcgct ctccactcgc cggagatacg cggccggaac caggcaaggc
     5461 ctcggatgtc attcgcatcg tcaggggcaa gcgtggccag ctcgccatgt cttgcgagct
     5521 catattgcgc tttggctacg gccagatcgt accctgggta acgcgtctgg gggacggcag
     5581 cggcggactt tgcgccgtcg ccgggcccga catgacggtg ctgcgcacgc caatcgaatt
     5641 gcgtggagac aatttcaaga ttgccggcag ctttgtcgtg tcggaaggcg aaacggtgcc
     5701 tttcgtcctc agctacggcc gctcctatcg agcgccgccc gaaccgaccg atccgctggc
     5761 cgcattgtcc atgaccaagg agctctggcg cgactggctg cagggctgtc gcgaagttgg
     5821 caagtggtcg gatgctgtgc gccgctctct gattacgctc aaagcactat cctatcggcc
     5881 aaccggcggc atcgtcgcgg cgccaacgac ctcactgccc gagcaactgg gtggaacgcg
     5941 caattgggat tatcgctact gttggctgag agatgcgaca ttcaccttgt tgagcctgat
     6001 gaacggcggt ttttatcagg cggcgcggga ttggagccaa tggctgttgc gggccgtcgc
     6061 cggtactccg cagcaaatcc agatcatgta cgccatcgac ggcgagcggc gactgaccga
     6121 actggagctg ccctggctac ccggttatca gggcgcgaag ccggtgcgga tcgggaatgc
     6181 cgcatccaat caattgcagc ttgacgtcta tggcgagatc atggacgcgt tctatcaagg
     6241 gcgccggggt ggactggatg cgctcgacct ggatttacaa cgcgccctcc tcgatcacct
     6301 cgcccagata tggcgccaac ctgatgaagg catctgggaa gtccggggtg ggcgccagca
     6361 cttcacgtat tccaaagtca tggcctgggt cgcgttcgat cgcgccatca agctgggcga
     6421 ggagttcgca attgaaggcc cgatcgatcg ctggaaagcg atacggcagg cgattcacaa
     6481 cgaggtttgc gagaaggcgt tcaatccggg cgtcggcgca ttcactcaat attatggtgc
     6541 ggattgcctc gatgcgagcg tgctcctcat gccgctcgtc ggttttctgc cggcaagcga
     6601 tccacgtgtc cgagggacag tggcagcgat agagcgccac ttgatggtgg atggccttgt
     6661 catgcgctac gacacgcatg gcaccaagga cggattgccg cccggcgaag gcgccttcct
     6721 ggcctgcagt ttctggttgg tcgataatct tgtcatgcag ggccggatgc aggaggcgga
     6781 acggcttttc gaacggctct gtggactttg caacgatgtc ggcttgcttt cagaggagta
     6841 cgatccatcc gccaagcgcc tggtcggcaa ttttccgcag gcattctcgc atattgcgct
     6901 ggtcaatagc gctcacaatc tcgtgctttc gcagaactcg ctcaaacagc gcgccgggca
     6961 cgatcgatcg gcctgattgg ttcatgccgg cgacgaagtc ataggtttga tgcgaaccgg
     7021 gctcctccac cgcaagagag gcacagaaaa atgactcatt ctcccgacca gggaagcggt
     7081 gcaacattcc gagcctatcg cgatgatttc cacgcgttgc tcggctccga ttttcagcgc
     7141 cccattctga tcgaaacgaa cgcccatgaa gggccagtct acgtcgccgc agagcatgcg
     7201 ctgtacttca cgacggtacc tgagcccggg cctaaaaata ttgccataag gcgcgtgcaa
     7261 ttggtgggcg aagagtttcc gttcgaggca cgaccacctg aaaccgttcg gttcccctcc
     7321 aatatggcga acggcatgac gctcgaccgc gaggggcggc tgctgatttg tgagcaaggc
     7381 ttcaaggaaa ccccggcgcg gatcagccgc atggacctcg aaacccgtgc ggtcgagacg
     7441 gtggtcgatg aatggcgcgg acttcagttc aactcaccga acgacgtggt ggtgaagagt
     7501 gatggtacgg tgtggttcac cgatccgaac tacggcgagc tgcaaggctt caagggcacg
     7561 cctaaggtcg gcccatacgt ctatcgccac gatcctggca cgggtgaaac tgcggtggtc
     7621 gccgacaact tcaacaagcc caacggactt gccttctcgc ccgacgagtc ggtgctttac
     7681 attacggata cgggcgccaa tcaagccccg ggcacctatt tcgtcggctt accgcatcat
     7741 atacgtgcct tcgacgttca tggtgggcgg cacttgcgaa acgaccggtt gtttgccgtt
     7801 atcagtcccg gagttccgga cggcatcaag gtggacattc aaggccgcgt ctacacctcc
     7861 tcggcaactg gcgtgcaggt gttcacgccg gatggcgatc tgctcggcga aatcatggcg
     7921 cccggcgtgg caaatttcac gttcggcggt ccgagaaaca acgtcctgtt cattctgggg
     7981 gatacagtga tctggcaggc gcagcttcag gtgagcggaa acgtcacttg ttgaatacga
     8041 tacctatctt atgaccctaa cgggtcatcc cggcgcctgt cgccgggatc caccattccg
     8101 ctgacgccag agcttgtgga gaactggata ccggggacat tgccccggta tgacatattt
     8161 gggtagactt cgcttagcca atattgcgat ctacacccga gaagctcgca cgcaatctgc
     8221 gggccccgtc aagccaatcg ctgcagcagg tgatcgccta cccggatcgc atttgcgatg
     8281 gcagtcagag acgggttcac ggcaccgatg ctgacgaaaa agctggtatc caccacgtac
     8341 aggttatcga gctcgtgtgc cttgcagttc acgtccagca cggatgtctg cggatcgcgc
     8401 ccgaaacggc atgttccggc ttggtgtgcg caacctgcga tggggatttc ggttttcata
     8461 tacgcattgc gcgggatcag gtgattgggg tgcattccca ggtgatttag catcgatttg
     8521 agcttctcat agagcttctg cgcgggcact tggttattcg gcgtatagct caggatcagc
     8581 ttgccagacc ggtccacggt cacgcgattt tccggatcgg gcagatcctc cgtcgtgagc
     8641 cagaaatcga cggcgtgata ggcgatctca tcgagcgcgc gcatcgggag caaggccgtc
     8701 tcaatcggtt tctcgccacg atacatcggg cccaaagact tgcccatcat ctggatatta
     8761 cccacagggt aatcgaaacc atccatgccg aaatagaaat cgttcagcgc cagggttttc
     8821 tggaacatgg tgggattggg ctcgcgcgat atcgcgagaa cggcctggct attgtgaaac
     8881 atgtagttcc gtccaacctg gtccgagccg ttggcgagcc cgttcggatg cttgtcgttg
     8941 gccgacatca gcagcagctt ggcgctattc gcggcgccgc aggacacgac gatgatgtcg
     9001 ccgcgaaaag tgtcaagtac gccttcgacc tctgctacga ctgaggtgac tgctgtgccg
     9061 gcagaattgg tttcaagttt gagcgcccgc gcattgcgga ggagcatcac gttggggtac
     9121 ccgagggcgg gtcggactgc cacgacttcg gcgtccgatt tggcgtgcac caggcaggga
     9181 aagccgtcac acgtctggca ccggatgcag gtgctatacg ccatgttgct ttcgtccagc
     9241 atgatcgcgg ccggcgccgg aaatgggtga taaccggccg cggcgatgtc atcgaatagc
     9301 tgctgtatgc gtggctcgtg agagagcggg ggacacggat agggggcgct ggccggaggt
     9361 tcggtcgggt cctgcccgcg cagcccgtga acgtgataca tctgctcggc tttggtgtaa
     9421 tagggctcaa attcctcata cgtgatcggc catgccggcg aaattccatc gtaatggcgc
     9481 agctcgccga aatcctcctt gcgcaggcga tagagcgctg caccgaacat tttggtcgcg
     9541 ccgccgacga aataatgcac ctggggctga aacggccggc cgttgcggtc gtaccatgtc
     9601 tcgggcgaga catagcggtt ctcccggaag acagccgtcg catcccagtt cagcgcctca
     9661 cgtttgagcc agtcgccgcg ctcgaggatg aggatgctct ttcccgaggg cgccagatgc
     9721 cgtgcaagcg tgccgccgcc ggcgccgctg ccgatgataa caacatcgaa atgtttatcc
     9781 atagcgggtc gaattcgcta caggccgaca ctgctctgca taaggccacc gtccgcgaag
     9841 atgctggtgg cggtgagata gctcgcgccg tcgccggcaa ggaaagcgac cacgctcgcg
     9901 atctcctcgg ggcgcgccat gcgcccgagc ggaatggcgg aattgagctt ctggagcttg
     9961 gcggggtctt ccatagtggg aagattgatg ggcgtcgcta ccgcgcccgg tcccacgccg
    10021 acgaccagga tattgtgagg cgcgagctcg actccggccg tgcgcgtgag catccgcata
    10081 ccgcccttcg cgagacaata agccgtattg ccgggcatcg gccagtcctc atggaccgaa
    10141 ctgatattga taatgcggcc gccatcgccc tgcttgatca tctgtttggc agcgatttga
    10201 gtaccgaaaa aagcgctttt aaggttaatt cgcaaaaccc tgtcatattg atcctccgtc
    10261 gtatcgagga cggaggtgcg cgtttcgatg ccggcgttgt tgaccatgat gtctagtcgc
    10321 ccgaaatggg caaccgccgc atcgacaagc ttctgaagat cgtcaatttt gctgacgtcg
    10381 gcatcgacgc cgatggcctg gccgccaagc ttggcgatct cgcgctccaa ttccgcggtg
    10441 gcctcgggat ggaccacata gtcgataaca attccggagg ctccttgtcg agccagctca
    10501 agaacgatgg cctgaccaat tccgctattg ccgccggtaa cgatcgcgac tttgttctgc
    10561 aatgacatga gcggtatctc cacgaatgtg ggtgcggtgg aggaaggtga tttccgtgtc
    10621 gctgtcctat ggtaggtcga ctccaccgcg cggaccgaac cactacagcg gtccgcctta
    10681 ggcgccgaac ttcacgatcg tggaaatcac gacggctatg acgaaggcag caaacagcac
    10741 cccatagaac acggcgcggc gcggtgcctt gtccagataa agctcaaacc gcctcgccca
    10801 ttccgccgga taatcccgcc cggtagcttg gtgctcaggc tgaggggacg gcggtcgggg
    10861 aaacgggtgc ctcgccttct ctgtcgacat cggaagtttc attggcgccg gcaaaacgca
    10921 gtggcaggca tttggtttca cgctggccct gttgaaaaag cgaggctctg ggcgccttta
    10981 gaaattcacc atagcggcgt cccgatattt tcttgtgacc agcaaatttg gcgacccggc
    11041 ccgccctgag ccgatagccc agcttcgcct tctcacccag aacaaccgca cgggatcggt
    11101 tcctgtgcgg ccacacccac acttcgcgga gccctgatac tcccccctac ccggcggtca
    11161 ggcttgcatc gtccctgatc tgagtgcata gtcgcatccg ataacgcata cgctaacttg
    11221 cacaggagcc tccatgacgc ctgccgcgcc tcttggtgcg cctcaggaaa ccggtcgcgg
    11281 gcagcgctcg accgagcttg cgtatctcca catcaagcag aagatcctcg acaacgaata
    11341 tccgccgggc gcgcacgtcc tcgagcagga catcgcggcc gagctgaagc tgtcgcggac
    11401 gccggtgcga gaggcgctgg tgcgcctgca gcaggacggg cttgtgaaga tcgtgccgcg
    11461 gcacggggtc cgcatctcgc ctctctcgcc cgccgacatg cgcgagatct acgaggttct
    11521 gatgagcctg gagccgacag ccgtcgagct gctcgcgcga cggcgcccgt cgctcagcga
    11581 gcttgcgccg ctggttgagg cctgtgatgc catggaggcc gccctctccc gccgtgagcc
    11641 ggacctgaaa gcctgggctg ccgccgacga gtccttccac atcaacctcg cgcgcatgtg
    11701 cggcaacagc cggctcgcgg ccatgatcat gacggtctgg gaccaagcac accgggcccg
    11761 catgttcacc ctcacgctgc gtccggtccc gcatcgctcc accgaggagc atcgcgccgt
    11821 ggtcgaggcc attctggccg gagacgcggc cggcgcacac aagctctacg ccgcgcatcg
    11881 ccgtcgcggc ggggtggagc tgatgtccat catcgaacgg cacggattgc agcatctatg
    11941 aaatcgaacg tcttccatgt cgtgcagatg ccgggcgacg gcatcggaat cgaggtgatg
    12001 caggcggcgg gcgccgtcct cgccgcggtc gaggcccgct atggcctcgg cttccgctat
    12061 gagacggtcc caggcggtgc ccattacttc aaggaaaccg gcacggttct gccggccgac
    12121 ggcatggatc gcgccaagcg cgccgatgcc atcctgttcg gagccatggg atggcccgac
    12181 attcgtttcc cggatggtac cgagatcgct ccgcagctcg acttacgatt cggtctcgat
    12241 ctctatgcgg gcgtgcggcc catccgtgcc atcccgggcg tgccgaccgc gctctcgcat
    12301 cccagagcca aggagatcga cctcgtcatc gtccgagagt cgacggaggg catgttcgcg
    12361 tcccgcggca agggcgtcgt catcgacgac cgcgaggcac gcgataccca ggtcatcacc
    12421 cgccgcacca cggagcgcct gaccgatttc tgctgccgac tggcacgccg gcgcaaggcc
    12481 gagcgcggca cgccggggcg cgtgaccctg gtggataaat cgaatgtgtt cgtgtcgttc
    12541 gcgttcatgc ggcaagtctt ccacgaggca gcggctaccc accccgacat cgaggcacgg
    12601 caccactacg tcgatgccat ggcactggac ctggtgcgcc ggccctggga cttcgacgtg
    12661 ctaccgatgg agaacctgtt cggcgacatc ctttcggatc ttgccgccgg cctgatcggc
    12721 ggcatgggat tcgcgccgtc agccgacatc ggcgatgctc acgcgctgtt ccagccgagc
    12781 catggatcgg cgccggacat cgccggcaaa ggcatcgcca atccgacggc catgattctc
    12841 tcggcggcga tgatgctcga ctggctcggc gagcggcacg gcagcgatag cgcgcgtgcg
    12901 gccgcccgcg ccgtcgaaaa aagcgtcgac ctcgtttttg gcagcgggcg ggtccgttcc
    12961 tgcgatatcg ggggcagc
//
LOCUS       DASVDU010000106        10647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1440672, whole genome shotgun sequence.
ACCESSION   DASVDU010000106 DASVDU010000000
VERSION     DASVDU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440672"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VF226_06630"
     CDS             <1..865
                     /locus_tag="VF226_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7073702.1"
                     /translation="DSVKQGQTLATLESIEAGEAYAAYRQALAEANVAKSAYERAERL
                     HKEQIIPGKEYQRARGEYEKTQATIEAAAAKLRMLGIEPAEAGKEAAASTFPVKAPLS
                     GTVIERKAVLGELAKTDEALFVVADLSRVWLIANIGEADLGRVRTGSVARARIPSQPN
                     EVFQGKVNHLSSVLDKETRTASAIIELDNSKGLLRPQMFATVEIESGAARKVLAVPES
                     AVTLVQGLPTVFVEEAAGFEARPVELGDRSGGSVVLKSGVKPGELVATEGIYALKARL
                     LKSQIGEGHAH"
     gene            875..3985
                     /locus_tag="VF226_06635"
     CDS             875..3985
                     /locus_tag="VF226_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX7073703.1"
                     /translation="MLNWIISVSLRYRLLVLLAFIGVIALGVQALRTVPLDAFPDVTP
                     NQVNVYTESPGLAAEDVERLLTTPIEASLAGLPGVEVIRSVSLFGLSYVGVYFRDDVD
                     IWFARRLVGERLTEVRERLPEGYGEPELGPNSSGLGQVFWYTIESADEKLSAMDLRTL
                     HDWSVRLALRTVPGVDDVTTWGGHEKQFQVLIDPTRLIKYQLSFKEVMEALVANNRQV
                     GGQFLNVGSEQYLVRGLGLVGNTEDIGNIVVATRQGVPIYIKNVARVVEGPAPRFGAV
                     TRDGKEVTLGIALSRIGENVKTVSEAVKAKLETAKQALPKSVSINPVYDRTDIVQKAL
                     DTSSRTLIEGAILVAIVLFLFLGELRSALVVIATLPLAMLIAFILMQRFGMSANLMSL
                     AGLAIGIGMMVDGAVVMVENAFRHLAHKGRGTSRAEVVGHAAREVAQPIAFAILIIIV
                     VFLPLFSLTGLEGKLFKPMAYAISFAMAGSLILSLTLVPVLSSLMLKPKPESDPWLLR
                     ISRRIYTPMLDWALGHRKAVAGTALVLLGLSLAAFPFLGKEFMPTLQEGEIMMRVTGI
                     PATSLDETVRVSQRMSAVLHEKFPQTKSVLATMGRAEKGETTDVNYMEVLVAVKPESE
                     WPERIPMPELAREMQEALEKAVPTVVFSATQPIQMRVEELISGVRATLALKVYGPDLT
                     TLDRLAARMKQSLAGVEGVADLSLEANQGKPQIVIRVDREAAARLGVNADEVLELVQA
                     GIGGRPVSTLLDGTRRFDIAVRFDEPFRGSIESIRNLPVRTGSGALVPLSRLATVETD
                     EGYTFVRREALQRYAVIQMDVRGRDIDSFVQDASAKVQADVQMPEGYWMEWGGAFENQ
                     QRALARLAIIVPITIALIFLLLYTAFNSLLYAGLIIANVPFAIIGGVVALAVGGFYVS
                     VPSAIGFIAVFGVAMLNGIVLVSFIHEQWVSRDLTLRQAVREGTLLRLRPVLMTASVA
                     ILGLVPMLLSGGVGAETQRPLAAVVIGGLFTSTALTLLVLPVMYEWVMSRRHGEKAPP
                     SLREAT"
     gene            4190..4582
                     /locus_tag="VF226_06640"
     CDS             4190..4582
                     /locus_tag="VF226_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073704.1"
                     /translation="MKLAAAVFAVALGMTTPAYAADKHGGHEVEIGKYHVELVVKDRD
                     LSLYVRDQADKPVNPKTVKASANVLSGKDKATVQLAPGTGEALSGQAPFSIAKDAKVI
                     VTFSVDGGKSEQARFSLGQKQEHRGHKH"
     gene            4616..5122
                     /locus_tag="VF226_06645"
     CDS             4616..5122
                     /locus_tag="VF226_06645"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7073705.1"
                     /translation="MLTRGAVYLMCGFLTLGIPAWAQRSPPTEARALDIVRDDADVLS
                     SEEDRKLTALLQAVLDQTGVKVLVVVVPTTAPDDGERYGGRLVEHWAKRGAVDPAQTV
                     IAVLAVDDRNLSVLAGRGLPGLQRELAGGGALRFLVPYLKEERYFDAMMALGKWLSGR
                     IRNKGDRT"
     gene            5126..7462
                     /locus_tag="VF226_06650"
     CDS             5126..7462
                     /locus_tag="VF226_06650"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7073706.1"
                     /translation="MEKSRFDLELLLPEVPDDRDACIGRLRDALEARAGIEAAHLLRH
                     EEAPQQRLCIHFNPSIVSLQDVRRMALQAGTDLQQRYGHLLASIPPRPARRARRLAAE
                     FARLPGVLEAAVAADGTVRIEFDRQLADEAAIRAFLETHDRAREDHEGHAHGGRAELV
                     FVGLCGLTLLTGWMLERFSGIDSRVGLAFLLAAYFFGGFFTLREAFASVRAGRFEIDS
                     LMLVAAAGAAALGEWAEGALLLFLFSLGHALERYAMGRARRAIEALADLAPQTAIVRR
                     DGVVAEVAVADLRIGDTVLVKPNERLPADGLVERGESSVNQAPITGESVPVDKRTGDT
                     AFAGTINGAGALEIRVTRAAADSTLSRVVKMVREAQTNQSPTQQFTDRFERIFVPAVL
                     GGASLLLASWLVVDEPFSASFYRAMAVLVAASPCALAIATPSAVLSGIARAAGGGVLV
                     KGGGPLENLGRLTAVAFDKTGTLTEGKPRLTDVVAGPGAGEQELLRVAFAVESQSDHP
                     LAAAVVGGVLDRLGQSPADEATELRSITGRGVQAKVGGQVTHVGKDVLFSEIGGPPPP
                     DALRGAIEELKRNGRTTMVVRQGERYLGVLGLMDTPRVTAKAVVARLHSLGIRRTIMI
                     SGDNQSVADAVAQSIGIDDPRGDLMPEHKVEAIRQLRKEGGVAMIGDGVNDAPAMANA
                     TVGIAMGAAGSDVALETADVALMADDLERLPFAIGLSRSASRIIRQNVWVSLGIVAFL
                     VPATIFGLGIGPAVLVHEGSTVLVVFNALRLLAYSEPL"
     gene            7492..7953
                     /locus_tag="VF226_06655"
     CDS             7492..7953
                     /locus_tag="VF226_06655"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7073707.1"
                     /translation="MFALAARAEELRPLPPLLHTVSDEAGALSVDQGRALSRSAEDIF
                     DRTGVRVIIVIAETTRPEEIEDYTERLGQRWKRERDLVPERSIFVVLSVRDREFQVMP
                     GRGLEFVDRELKKPEAYAGLSPLFRQGRYFDALMTVNQRLLEIVLKQKSRS"
     gene            complement(7987..9075)
                     /locus_tag="VF226_06660"
     CDS             complement(7987..9075)
                     /locus_tag="VF226_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7073708.1"
                     /translation="MKLGRPARELIVSESDRAQLESIARSQSLPAGLARRAQMVLRMA
                     DGESGSAVARRFRVSRPTVSLWRKRFSEAGIKGLHNEIKPGRPRSTSEEAIAKLINTA
                     LQTKPKGKTHWSRRTLADATRLSKSTVHRYLTLFGVQPHRSRSFKLSSDPFFIEKVRD
                     IVGLYLNPPDHALVLCVDEKSQVQALERTQPVLPMGLGYVEGITHDYVRHGTTTLFAA
                     LDIANGSVLTQCKARHRHQEFLSFLRHIESNVPSHLDVHLICDNYGTHKHAKVKAWLA
                     RRPRFHLHFTPTYSSWLNQVERWFALITNQAIRRGSFDSVTDLKRKIQEFVEHYNRHP
                     RPFAWTATAESILAKLERLCKVINGTQH"
     gene            complement(9304..9528)
                     /locus_tag="VF226_06665"
     CDS             complement(9304..9528)
                     /locus_tag="VF226_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073709.1"
                     /translation="MPAGILATGLAGVYTGRLERSGAVPDLDGRVDDFLMLLQSAPHS
                     VGGEAPVVSHMQIGHPRVIALSAAFDQAQP"
     gene            9533..9733
                     /locus_tag="VF226_06670"
     CDS             9533..9733
                     /locus_tag="VF226_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073710.1"
                     /translation="MLGFVSMVAGAATGLVYPVWAMAAMAASVTAIFLNSLWGRPRLF
                     VDAILSVGRPAGAANPQAAEVT"
     gene            complement(9791..10258)
                     /locus_tag="VF226_06675"
     CDS             complement(9791..10258)
                     /locus_tag="VF226_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073711.1"
                     /translation="MTGNPLLDLAVLPIAFGLIGFIEPCSLASTLIFIKVIEGKRAAT
                     RIAQTLAFAAGRAAFIGLLGAIAVLVGTAFFGFQKAAWAILGLLYAALGIAYLTGRIG
                     WFMRSFGPSLARISSTGTRCDGLPRRCRDDNPTLFFSPAARDLRARSTGCCRP"
     gene            complement(10279..10617)
                     /locus_tag="VF226_06680"
     CDS             complement(10279..10617)
                     /locus_tag="VF226_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073712.1"
                     /translation="MKKRVNGLMAAASLLLLVQTPAFSEEKTEGKVVGTRLTHCDMNV
                     KIGGCAGTMKLERTAGEKKEELSFNVPLGTPITRGSEAVYLPALRGRTVVVTHVTEKN
                     ERVAKAIELK"
BASE COUNT         1966 a         3220 c         3510 g         1951 t
ORIGIN      
        1 cgattccgtc aagcaagggc aaacgctcgc cacgctggag agcatcgaag cgggagaagc
       61 gtatgccgcc tacaggcagg cgctggcgga agctaacgtc gcgaagtcgg cctatgagcg
      121 cgccgagcgg ctgcacaagg aacagatcat tccaggcaag gaatatcagc gcgcgagggg
      181 cgagtacgaa aaaacgcagg caacgatcga ggcggcggcc gccaagctgc ggatgctggg
      241 catagagccc gcagaggcag gcaaagaagc ggcagcgtcg acgttccctg tgaaggcgcc
      301 gctgtctggg acggtgattg agcgaaaggc cgtgctcggc gagctggcga agaccgatga
      361 agcccttttc gtagtggccg acctgtccag ggtctggctc atcgccaata tcggtgaagc
      421 cgatctcggg cgcgtgcgca cgggctcggt ggcgcgtgcg cggattccca gtcagcccaa
      481 cgaagtgttc cagggcaagg tgaaccacct gagctccgtg ctagacaagg aaacccgcac
      541 ggcgagcgcc atcatcgagc tcgacaattc caagggcctc ctgcgtccgc agatgttcgc
      601 cacggtggag atcgagagcg gcgcggctcg caaggtattg gcggtgcccg aatcggcggt
      661 aacgctcgtg caggggttgc cgacggtgtt cgtcgaggag gcggcgggat tcgaagcgag
      721 gccggtggaa ctcggcgacc gttccggcgg ctcggtagtc ctgaaatccg gcgtcaagcc
      781 cggcgagttg gtagccacgg aaggcatcta tgccctgaag gcgcggttgc tgaagtccca
      841 gatcggggaa ggccatgcgc actgaggcca tcccatgctg aactggatca tctccgtttc
      901 gctgcgttac cggcttctcg tattgctcgc gttcatcggc gttatagccc tcggggtgca
      961 ggcgctgagg accgtgccgc tcgatgcatt cccggacgta acgcccaatc aggtgaacgt
     1021 ctacacggag tcgcccggcc tcgcggccga agacgtcgag cgcctgctca cgacgccgat
     1081 cgaggcatcg cttgccggct tgcctggcgt cgaagtcata cgctcggtgt cgctcttcgg
     1141 cctgtcctac gtcggcgttt actttcgcga cgatgtggac atctggttcg cccggcgcct
     1201 ggtaggggag cgacttaccg aagtaaggga gcgtctgccc gaggggtatg gcgaaccgga
     1261 gctgggaccg aacagctccg gcctcggtca ggtgttctgg tacacgatcg agtcggcgga
     1321 cgagaagctt tccgccatgg atctgcgcac gctccacgac tggagcgtac ggctcgcact
     1381 ccgcacggtt cccggcgtcg atgacgtgac gacgtggggc ggacacgaaa agcagttcca
     1441 ggtgctgatc gatcccaccc gccttatcaa gtaccagctg tctttcaagg aggtcatgga
     1501 ggccctggtc gcgaacaacc ggcaggtagg cggccagttc ctgaatgtcg ggtccgagca
     1561 gtacctcgtg cgcggtctcg gcctcgtggg gaacaccgag gacatcggca acatcgtggt
     1621 ggcgacccgg caaggggtcc cgatctatat caagaacgtt gcccgggttg tcgaaggacc
     1681 ggcgccgcgc ttcggcgcag tcacgcgcga cggcaaagaa gtgaccctcg gaatagcgtt
     1741 gtcccggatc ggcgagaacg taaagaccgt ctccgaggcg gtgaaggcca agctcgagac
     1801 cgcgaagcag gcgctgccga agtccgtgtc gatcaacccg gtctacgacc ggaccgacat
     1861 tgtgcagaag gcgctcgaca cgtcgagccg cacgctcatt gaaggggcca ttctggttgc
     1921 catagtgctg ttcctgtttc taggcgagct gcgctcggca ctggtggtca tcgccacgct
     1981 gccgcttgcg atgctgatcg cattcattct gatgcagcgc ttcggcatgt cagcgaacct
     2041 catgtccctg gcgggcttgg ctattggcat cggcatgatg gtggacggcg cggttgtgat
     2101 ggtcgagaac gctttccgcc atctcgctca caaggggcgc ggcacgtcgc gcgccgaagt
     2161 ggttgggcat gcggcgagag aggtggcgca gccgatcgcc tttgcgatcc tgatcatcat
     2221 cgtcgtgttc ttgccactct tctcgctcac cgggctggag ggcaagctct tcaagcccat
     2281 ggcctacgcg atctcgttcg ccatggcggg ctcgctcatc ctgagcctga ccctcgtccc
     2341 ggtgctttct tcgctcatgt tgaagccgaa gccggaaagc gatccatggc tgctgcggat
     2401 atcgaggagg atctacacgc cgatgctcga ctgggcgctc gggcatcgaa aggcagtcgc
     2461 gggtactgcg ctcgtgcttc tcggtctttc gctcgctgcg ttcccgttcc tcgggaagga
     2521 gttcatgccg acgttgcagg agggcgaaat catgatgcgc gtcacgggta ttccggcgac
     2581 ctcgctggat gaaacggtgc gcgtatcgca gcgcatgagc gcagtgttgc acgagaaatt
     2641 tcctcagaca aagtcggtgc tcgcgaccat gggccgagca gagaagggcg agacgaccga
     2701 cgtgaactac atggaagtcc tcgtcgcagt gaagccggaa tccgagtggc cggaacgcat
     2761 ccccatgccc gagctcgcgc gcgagatgca ggaggcgctg gagaaggcag ttccgacggt
     2821 ggtgttctcc gctactcagc cgatccagat gcgcgtcgag gagctgatat ccggcgtgcg
     2881 agctacgctt gcgctcaagg tctacggccc ggacttgacg acgctggatc gtcttgcggc
     2941 acggatgaag cagtcgctcg ccggcgtcga aggtgtggcc gacctgtctc tcgaagccaa
     3001 ccaaggcaaa ccgcagatcg tcatccgtgt ggatcgagag gccgccgccc ggctcggcgt
     3061 gaacgcggat gaagtgctcg agctggtgca agcagggata ggaggcaggc ctgtctcgac
     3121 gcttctggat ggcacccggc gctttgatat cgccgtgcgc ttcgacgagc cattccgcgg
     3181 cagcattgag tcgatccgca atcttcccgt ccgtacggga agcggggcgc tcgtgccgct
     3241 gtcgcgcctg gccacggtgg agaccgacga gggctacacg ttcgtgcgcc gcgaagcgct
     3301 gcagcgctat gccgtaatcc agatggacgt ccgcggccgg gacatcgaca gtttcgtgca
     3361 ggacgcatca gcgaaagtgc aggccgatgt gcaaatgccg gagggctact ggatggaatg
     3421 gggcggcgcc ttcgagaacc agcagcgcgc cctggcgcgc ttggcgatca tcgtgccgat
     3481 caccatcgct cttatcttct tgctgctcta tacggccttc aactcgctgc tctacgccgg
     3541 gctgatcatc gccaacgtgc ccttcgccat cattgggggt gtggtcgcat tggccgtggg
     3601 tgggttctac gtttcagtgc cgtccgccat cggcttcatc gcagtctttg gcgtagcgat
     3661 gctgaacggg atcgtgctcg tatcgttcat ccacgagcaa tgggtatcgc gagacctgac
     3721 tttgcgccag gcggtccgcg agggaacgct gctgcgcctg cgcccggtcc tgatgaccgc
     3781 gagcgtcgct atcctgggcc tggtgccgat gctgctctcg ggcggtgtcg gtgcggaaac
     3841 gcagcgaccg ctcgctgcag tcgtcatcgg ggggcttttc acctccacgg ctctcaccct
     3901 gctcgtactc cccgtcatgt acgagtgggt gatgtcccgg cggcatggcg agaaagctcc
     3961 gcccagcctc cgcgaggcga catgaggccg cggcggatag tgatggtccc gttgataggc
     4021 ggccgcagct cgctcatcta cgacgccgag atggcttacc tggtttcccg cgacgttcag
     4081 ctcgacatca gtgcaggcgc gagggaccga ggcacaaatc caccgcggag ctttgtcggt
     4141 gcgggcgtga gcgtccgatt ttgatcaagc gaactccata ggagattcca tgaaacttgc
     4201 agcggcagtc ttcgcagttg ctcttgggat gacgacgccg gcgtacgcgg cggacaagca
     4261 cggcggacat gaagtcgaaa tcggcaaata ccacgtagag ctcgtcgtaa aggaccgtga
     4321 tctttcgctc tatgtccgtg accaggccga caagccggtc aacccgaaga ccgtgaaggc
     4381 gagcgcgaat gtgctttccg gcaaggacaa ggccacggtc cagctcgcgc ccggcaccgg
     4441 cgaggcgctg agcggccagg cgccattcag catagccaag gatgccaagg taatcgtcac
     4501 cttcagtgtc gacggcggga agagcgagca ggcacggttc tcccttggcc agaagcagga
     4561 gcacaggggc cacaagcact gacagtcggg ggcggcaaac gccgaggagg acaagatgct
     4621 gacaaggggc gcggtctatc taatgtgcgg tttccttacg cttggcatcc cggcttgggc
     4681 gcagcgctcg ccgccaacgg aggcgcgcgc gctggacata gttcgagacg acgccgacgt
     4741 actgtccagc gaggaagatc ggaagctgac tgcacttctt caggcagtcc tggatcagac
     4801 gggcgtgaaa gtgctcgtag tggtggtccc gaccaccgcg ccggatgatg gcgagcgcta
     4861 tggcgggcgc ctggtggagc actgggcaaa gcgcggagcg gtagatccgg cgcagacggt
     4921 gatagccgtg ctggcggtcg acgatcggaa tctatctgta ctcgctggac gcggtttgcc
     4981 gggattgcag cgggaactcg ccgggggagg agccttgaga ttccttgtgc cgtatctcaa
     5041 ggaagagcgc tatttcgacg caatgatggc gctagggaag tggctttccg ggcgcattcg
     5101 aaacaagggc gaccggacgt aagtcatgga gaaatcccgg ttcgacctgg agctgctgtt
     5161 accggaggtt ccagacgacc gggatgcatg cattggccgc ctgcgggacg cgctggaggc
     5221 tcgagccggc atcgaggctg cgcatctact ccggcatgag gaagcgcccc aacagcgact
     5281 gtgtatccat ttcaacccga gcatcgtgtc gctacaggac gtccgtcgca tggccctgca
     5341 ggcgggcacg gacctgcagc aacgctacgg tcatctcctt gccagcatcc cgccgcgacc
     5401 ggcacgcagg gcgcggcggc ttgctgccga atttgcgcgg ctgcccggtg tcctcgaggc
     5461 cgccgtagca gcggatggca cggtgcggat cgagttcgat cggcagcttg ccgacgaagc
     5521 agcgataagg gcgttccttg agacccacga tcgcgctcgc gaagatcacg agggtcatgc
     5581 gcatggcggc agggccgagc tcgtctttgt cgggctctgc gggctgacgc tactcaccgg
     5641 gtggatgctg gagcgctttt cgggcatcga ttcccgcgtc gggctggcat tccttctggc
     5701 ggcgtacttc ttcggcgggt ttttcacgct gcgcgaagcc tttgccagcg tccgtgccgg
     5761 ccggttcgaa attgattcgc tcatgctggt tgcggcggcc ggtgccgccg cgctgggcga
     5821 gtgggctgaa ggggcgctcc tgcttttcct attcagcctg ggccacgcgc tcgagcgcta
     5881 tgccatgggc agggcccggc gggccatcga agcgctcgcg gacttggcgc cgcagaccgc
     5941 gatcgtgcgc cgcgacggag tcgtagccga ggttgcggtg gccgacctgc gcatcgggga
     6001 tacggtgctc gtcaagccga acgagcgcct gccggcggac ggactggtgg aaaggggcga
     6061 aagcagcgtc aatcaggcac ccatcaccgg agagagcgta cccgtcgaca agcggaccgg
     6121 agatacagcc tttgcaggca cgatcaacgg ggcaggggcg ctggaaatcc gggtaacgcg
     6181 cgccgccgcc gattccaccc tgtcccgcgt ggtgaaaatg gtgcgcgagg cacagacaaa
     6241 tcagtcgccc acgcagcagt tcaccgaccg cttcgagcgc atctttgtac cggcggtcct
     6301 cggcggggcg tcgctcctcc ttgcctcctg gctggtggtc gacgagccgt tcagtgcaag
     6361 cttctaccgc gcgatggccg tgctcgttgc ggcgagcccc tgtgccctcg ccatcgccac
     6421 accgagcgca gtgctgagcg gcatcgcgcg cgccgccggg ggcggggtgc tggtcaaagg
     6481 cggagggccg ctcgaaaacc tcgggcgcct caccgccgtc gcattcgaca agactggcac
     6541 cctcaccgaa ggcaagccgc gattgactga cgtcgtggca gggccaggtg ccggggagca
     6601 agagctgctg cgcgttgcct ttgcggtaga gagccagagc gatcacccgc tggcagcggc
     6661 cgtcgtaggc ggcgtacttg accggcttgg ccagtcgcca gccgatgaag caacggaatt
     6721 acgcagcatc accggacggg gagtacaggc gaaagtcgga ggccaggtca cgcacgtcgg
     6781 caaggatgtg ctcttctccg aaatcggagg gccgccgcct cccgatgcgc tgcgcggcgc
     6841 tatcgaagag ctgaagcgca acggccgcac caccatggtg gtacggcagg gggagcgcta
     6901 cctaggcgtc ctgggtctga tggacacccc gcgagtgacg gccaaggccg tcgtcgcacg
     6961 cctgcattcc ctaggcattc ggcggacgat catgatctcc ggcgataacc agtccgtagc
     7021 cgatgccgtg gctcagtcca tcggcatcga tgatccgcgc ggcgacttga tgcccgaaca
     7081 caaggtcgag gccatcaggc aactaagaaa agaaggcggc gtcgccatga taggcgacgg
     7141 tgttaacgat gcaccggcca tggctaatgc cacagtcggc atcgcgatgg gtgcggccgg
     7201 gtccgacgtc gccctggaaa cggccgacgt ggcgttgatg gcagatgacc tggaacgcct
     7261 gccgttcgcc atcggcctga gccgctcggc aagccgcatc atccggcaga acgtctgggt
     7321 tagcctcggc atcgtcgcct ttctcgttcc ggccacgatc ttcggcctcg gcatcggtcc
     7381 cgccgtgctc gtgcacgaag gatccacggt gctcgttgtc ttcaacgccc tgcgtctgct
     7441 cgcctattcg gaaccgctat gagaagcgtt gtcgcaacgc ttgtgctcct catgtttgcg
     7501 cttgctgcgc gtgccgaaga actgcggccg cttccgccgc tgctccacac agtaagcgat
     7561 gaagcaggag ctctctcagt agatcagggt cgagccctat cgcgatccgc agaagacatc
     7621 tttgaccgaa ccggtgtgcg agtcattatc gtcattgcag aaaccacgcg tcccgaggag
     7681 atcgaagact acacggagcg actagggcag cgctggaagc gcgaacgtga tctcgtcccg
     7741 gagcgaagca tcttcgtcgt gctctctgta agagaccgcg agtttcaggt catgcctggc
     7801 agaggcctcg agttcgtcga tcgcgagctt aaaaagcccg aagcctatgc gggtttgtct
     7861 ccgcttttcc ggcaaggtcg gtatttcgac gcactcatga ctgtcaacca gcgtctactg
     7921 gagatcgtcc tgaagcagaa gagccggagt tagcagtcgt tccgcgctcg actgggccgt
     7981 gtacggctag tgttgtgtcc cgttaatgac tttacagagt cgttcgagct tagccaggat
     8041 ggactctgcg gtagcggtcc aggcaaaggg ccgtggatga cggttgtagt gctcgacgaa
     8101 ttcctgaatc ttgcgcttga gatcggtgac cgagtcgaac gatccgcggc ggatcgcctg
     8161 atttgtaatc aaggcgaacc agcgctcgac ttgattgagc caggagctgt aggtcggggt
     8221 gaaatgcaaa tggaaccgcg gccgccgagc cagccaagct ttgaccttgg catgtttgtg
     8281 cgtgccatag ttgtcgcaga tcagatgcac gtcgagatgc gacggaacgt tgctttcgat
     8341 gtggcgaaga aaggagagga attcctgatg gcggtgacgc gctttgcact gcgtcagcac
     8401 gctgccattg gctatgtcca gagcggcaaa cagcgtcgtc gtaccgtgtc ggacgtaatc
     8461 gtgggtgatg ccttcgacat aacccaaacc catcggcaac accggctggg tgcgctccag
     8521 cgcctgcacc tggctcttct catccacaca cagcaccagc gcgtgatccg ggggattgag
     8581 ataaaggccg acgatgtcgc gcaccttctc gatgaagaac ggatcgctcg agagcttgaa
     8641 gctgcggctg cgatgcggct gcacgccaaa cagcgtgaga tagcgatgca ccgtgctctt
     8701 ggagagccgc gtggcatcgg ccagagtacg ccgcgaccag tgggtcttgc ctttgggttt
     8761 cgtctgcagc gccgtgttga tcagcttggc gatggcctcc tcgctggtgc tgcgcggccg
     8821 tccgggcttg atctcgttgt gcaggccctt gatgccggcc tcactgaagc gcttgcgcca
     8881 cagcgatacc gtcgggcggc tgacccggaa acgccgcgcc accgcgctgc cggattcgcc
     8941 atcggccatg cgcaaaacca tctgcgcgcg ccgcgccaac cccgcaggca ggctctgcga
     9001 gcgcgcaatc gattcaagct gagctcgatc cgactcgctc acgatcagtt ccctcgcggg
     9061 tcttcccagt ttcatcgccg agcgctccct gtccaaagaa tacaacttgg acagcactcg
     9121 acgcgtaaag ttacatacgg gacatcacac tagggaagct ctgaataacc gatgaagcag
     9181 cgcgccgagt ggatcgtagt tgatgattcg ccagcgaaca tgctgaagac gcgcgccgta
     9241 ttcttagcaa tgccgctagc gcaccttggc aaaggactac ggaggattgg ccggcggttg
     9301 aatctacggc tgggcctggt cgaaagcggc cgacagggcg atgacacgcg ggtggccgat
     9361 ttgcatgtga ctcacgacag gagcctcacc gccaacggag tgaggcgccg actgaaggag
     9421 catgaggaag tcgtccacgc gaccatcgag atccggtact gcacctgacc gctcaagccg
     9481 gccggtgtac actcccgcca gcccggtcgc gaggattccc gccggcatat ggatgctcgg
     9541 cttcgtcagc atggtggcag gcgcggcgac cggtcttgtc tatccggtat gggcgatggc
     9601 ggccatggcg gcgagcgtca cggcgatctt cctcaactcg ctctggggaa ggccgagact
     9661 gttcgtcgat gccattctca gcgtcggccg gccggccggg gcggcaaatc cgcaggcggc
     9721 agaagtaacc tgagcaacca catgaccgca cccatgctta ccatcggcaa ggtagccgag
     9781 caatctcagg tcacggccga cagcatccgg tactacgagc gcgaaggtct cgtgcggccg
     9841 gcgagaaaaa cagagtcggg ttatcgtctc tacatcgacg aggcagtccg tcgcatcgcg
     9901 tgccggtgct ggagatgcgc gcgaggctcg gcccgaacga gcgcatgaac cagccaatcc
     9961 tgcccgtaag gtatgcgatg cccagggccg catagagcag gccaagaatt gcccaggcag
    10021 ctttctggaa gccgaaaaac gccgtgccga ccagcaccgc gatggccccg agcaggccaa
    10081 tgaaggcggc gcgtccggcc gcgaaggcga gcgtttgcgc aatgcgcgtc gcagcgcgct
    10141 tgccctcgat caccttgatg aaaatgagag tcgaggccag agagcagggt tcgatgaaac
    10201 cgatcaggcc gaaggcgatc ggcaggacgg cgaggtcgag gagcggattg cctgtcaccc
    10261 cgtctcgctc cgccagtgct acttaagctc gatcgccttg gcgacgcgct cgttcttctc
    10321 ggtaacgtga gtcaccacga ccgtcctgcc gcgaagcgcc ggtaagtaga ccgcttcgct
    10381 gccccgcgtg atgggcgtgc caaggggaac attgaagctc agctcctcct tcttctcacc
    10441 tgcggtgcgc tcgagtttca tcgttccggc gcagccgccg atcttcacgt tcatgtcgca
    10501 atgggtgagc cgggttccga cgaccttgcc ttcggtcttc tcttcgctaa acgccggtgt
    10561 ctgcacgagc agcagcaggc ttgccgccgc cattagtcca ttcactcgtt tcttcatgac
    10621 ttacggttcc ccttgggcgt gcggccc
//
LOCUS       DASVDU010000107        32944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_31188, whole genome shotgun sequence.
ACCESSION   DASVDU010000107 DASVDU010000000
VERSION     DASVDU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 32944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32944
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31188"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF226_06685"
     CDS             <1..732
                     /locus_tag="VF226_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7073713.1"
                     /translation="GSCVSALLGGIGANWPAPPADWINAVKAKKEENIAKMAPKLMKN
                     TAPMDFHSALGALRNVIKERPDAILVNEGANTLDLARGIIDMYQPRKRLDVGTWGVMG
                     IGMGFAIAAAIETGKPVLAVEGDSAFGFSGMEVETICRYKLPVCVVVFNNDGIYRGVD
                     VNPTGGPDPSPMVFVKDARYDKIMEAFGGAGAHVTTPDELYRAVSAAMDSGKPTLINA
                     VIDPAAGTESGRIGNLNPQSVVKKK"
     gene            746..2017
                     /gene="frc"
                     /locus_tag="VF226_06690"
     CDS             746..2017
                     /gene="frc"
                     /locus_tag="VF226_06690"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX7073714.1"
                     /translation="MKALEGVRILDFTHVQSGPTCTQLLAWFGADVIKVERPGVGDIT
                     RGQLQDLPKVDSLYFTMLNHNKRSITLDTKHPKGKEVLERLIKACDVLVENFAPGVLD
                     RMGFSWERIQELNPRMIYASIKGFGPGPYEDCKVYENVAQCTGGAASTTGFDDGPPLV
                     TGAQIGDSGTGLHLALGIVTALYHRTHSGRGQRVTCAMQDGVLNLCRVKLRDQQRLAH
                     GPLTEYPQYSEGKPFGDAVPRSGNASGGGQPGWILKCKGWETDPNAYIYFITQAPVWD
                     KICDVIGEPDWKTHPDFATPQARLPRLKQVFDRIEQWTMTKTKFEAMEILNRYDIPCG
                     PILSMKELAEDQSLRATGTIVEVDHPTRGKYLTVGNPIKLSDSPADVKRSPLLGEHTE
                     EILRDVLGYSEEEIAEIREATEPPQKRAAAE"
     gene            2107..2325
                     /locus_tag="VF226_06695"
     CDS             2107..2325
                     /locus_tag="VF226_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7073715.1"
                     /translation="MLTPGHPKQPCVYILASSRNGILYVGVTSNLFGRVSLHKQDLID
                     NEEVRRPHARLFRDAHDHGGSDSAGEAA"
     gene            2291..2467
                     /locus_tag="VF226_06700"
     CDS             2291..2467
                     /locus_tag="VF226_06700"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073716.1"
                     /translation="MAEAIRREKQLKKWNRAWKVRLIEQVNPEWTDLWNETGEIRMRS
                     TGGQEPPADNLEQI"
     gene            2608..3468
                     /locus_tag="VF226_06705"
                     /pseudo
     CDS             2608..3468
                     /locus_tag="VF226_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
     gene            3574..5250
                     /locus_tag="VF226_06710"
     CDS             3574..5250
                     /locus_tag="VF226_06710"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX7073717.1"
                     /translation="MAFKSDIEISREAKMKPIAEVGKKVGIPPEALLNYGPTKAKVSF
                     DFIKTLEDRKDGKLILVSAINPTPAGEGKTTTTVGLGDGLNRIGAKAMCCLREPSLGP
                     CFGMKGGAAGGGYAQVVPMEDINLHFTGDFHAITSANNLLAALIDNHIYWGNSLGIDP
                     RRISWRRVMDMNDRALRSIINSLGGVSNGFPRQDGFDITVASEVMAILCLATDLQDLR
                     RRLGNIVFGTTRDRKPLFARDLKADGPMTVLLKDALMPNLVQTLENNPVFVHGGPFAN
                     IAHGCNSVLATKTALKLADYVVTEAGFGADLGAEKFFDIKCRKAGLKPSATVIVATVR
                     ALKMHGGIAKEDLKGENVEAVRKGAENLKRHLENVAKFGIPPVVAINRFVTDTDAEIA
                     AVKELAESMGTRAFLCTHWADGGKGTEELARYVVELCDSGKANFKPLYPDEMPLWDKI
                     RTIATELYGASDISADATVRNQLKDFEANGYGHLPICMAKTQYSFSTNPNLRGAPTGH
                     VVNIREVILSAGAEFVVAVCGDLMRMPGLPRVPSAEQMYVNEQGYIENLS"
     gene            complement(5320..6099)
                     /locus_tag="VF226_06715"
     CDS             complement(5320..6099)
                     /locus_tag="VF226_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073718.1"
                     /translation="MDLGIRGRSAIVCAASKGLGRGCAEALAREGVALTICARGKEAL
                     EATAEHLRGLGAASVTTVVCDVTTEEGRRALLAACPNPDILINNAGGPPPGDFKNFSL
                     DDWRKAVEGNMLTPIALIHATVYGMMERGFGRIVNITSSSVKAPIPTLELSNGARSGL
                     TGAVAALARKTVHRNVTINALLPGPFETDRLRATTRKIAEARGQPYEVVHEARKNEVP
                     AKRFGTPEEFGATAAFLCSAHAGYITGQNIVIDGGAFPGTL"
     gene            complement(6169..7137)
                     /gene="corA"
                     /locus_tag="VF226_06720"
     CDS             complement(6169..7137)
                     /gene="corA"
                     /locus_tag="VF226_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX7073719.1"
                     /translation="MLTIYHAHGAGLVKAQGALSAAEAVWIDLLNPIPAEEALVEREL
                     GIAVPTREEMAEIEVSSRLYHESGAYVMTATVLHRAETVSPESTAITFILTESKLVTV
                     RYAEPRAFSIFLARAQKGDTPIDSAMAILLGLLETIIDREADIIERLQADADRLAQTI
                     FDMKGGSATRTRRFEVILKQIGREGEIASKMRDSLLSLGRLLTYLSQVALRRKEAERV
                     RAQIRTASRDVQSLADHLNYLNARITFMLDASLGLVSIEQNQIIKLFSVVAVVLLPPT
                     LVASIYGMNFRTMPELDWTFGYPLALLLMVLSAVLPFVYFRRKGWL"
     gene            7262..8161
                     /locus_tag="VF226_06725"
     CDS             7262..8161
                     /locus_tag="VF226_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HEX7073720.1"
                     /translation="MKDVVELNRKYGVDNELGFCDIRGEPLAWIRNDACAATIALNGA
                     HLVSWYPYGHDEVFWVSSVRPSSSTMSQRGGVPVCWPWFGPHPTDPTKPQHGFVRNRR
                     WTVVSANSTSQVTELTLRTETTPEDAALWPHHAEVSLRFIAGATLCLELATRNTGSQA
                     FELTQALHSYFKVSDIANVAIEGFDGLEYLDKVDAYARKRQSGQITITGETDRIYLGH
                     TGPAIIHDSGLGRGIIVTKSGSTSSVVWNPWEERARQMGDLGEDGYRRMVCVETANAG
                     DDVVRIDPGHQHTLVANIQIARG"
     gene            8592..8879
                     /locus_tag="VF226_06730"
     CDS             8592..8879
                     /locus_tag="VF226_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073721.1"
                     /translation="MMLKSAIFATVLAAAAATGAASTASAAPAAAPAVLGGIQNTSDA
                     VQQVHWRRTRVVTRCIGGRRVLMRINWRGRVVSRRVIGRCWHHHRKHRHRW"
     gene            complement(8981..9235)
                     /locus_tag="VF226_06735"
     CDS             complement(8981..9235)
                     /locus_tag="VF226_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEX7073722.1"
                     /translation="MNRFEKLFGSHGEARVRYLDGEFQVLSPGEYVRCAVTGEQIPLQ
                     ELRYWSVELQEAYATPAASLQRYRELRARRSGASGKSKQD"
     gene            complement(9350..10969)
                     /gene="xseA"
                     /locus_tag="VF226_06740"
     CDS             complement(9350..10969)
                     /gene="xseA"
                     /locus_tag="VF226_06740"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7073723.1"
                     /translation="MAESATQQRTGANVAEYTVSELASAIKRALEDGFGFVRLRGEIS
                     GFRGTHSSGHCYFALKDDKAKIEAVIWRTTWQRLKVKPEEGMEVVAQGRITTYPNSSK
                     YQIVIESLEPAGIGALMAVLEERKRKLAAEGLFDEDRKKPLPFLPRVVGIITSPTGAV
                     IRDMLHGFDERFPVYVLVWPVRVQGETCSAEIAAAIRGFNALPAGGPVPRPDVLIVAR
                     GGGSLEDLWGFNHEGVVRAAAESEIPLISAVGHETDWTLIDLAADARAPTPTKAAEWA
                     VPKHSELVEGTATLALRLASSMRRVLEGARTHLKAAARGLPRREDLLALPRQRFDSVE
                     QRLGRALLANTRAHAMRHARVSSRLQPRLLEARLNRCRDRLEALSRHSRDALSRLAGA
                     RRSRFERTAGRLGIEAVRSRVDRGRERLASCGLRAEQAFRSRVAHERARLSGLSKLLL
                     SLSYQGVLERGFALVRDAEGHTIRSVAAVGLGTDIQVELADGCIDADVRDVRTTRGPS
                     RPTVSSIVDPAQVRQPRRKRNPPQGGQGSLF"
     gene            11075..12364
                     /gene="purD"
                     /locus_tag="VF226_06745"
     CDS             11075..12364
                     /gene="purD"
                     /locus_tag="VF226_06745"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEX7073724.1"
                     /translation="MNVLLLGSGGREHALAWAIAASPLLDTLYCAPGNAGIAEVARCV
                     PLDITDHAAVAAFCREKRIDLVVVGPEAPLVAGIADDLSAAGFKVFGPSRAAAQLEGS
                     KGFAKDLCRELGVPTAAYGRFSDAASAKAYAGQLPLPVVVKADGLAAGKGVVIAETRE
                     AADAAIDACLGGAFGTAGAEVVIEEYLRGEEASFFALVDGKHALPLGAAQDHKRAFDG
                     DTGPNTGGMGAYSPAPVMTPDMAERVMAEIIRPIVAGMAARGTPYKGVLFAGLMITPE
                     GPKLIEFNVRFGDPECQVLMMRLKSDLLPALMATVDGVLGNFDLRWHDDTALTVVMAA
                     NGYPGTPAKGSEIRGLEEAAKVPGVEIFHAGTRRDGTRIVSNGGRVLNVTARGRTVSE
                     ARDRAYEAISRIEWPEGFCRSDIGWRAIARERGGEPA"
     gene            12507..13385
                     /locus_tag="VF226_06750"
     CDS             12507..13385
                     /locus_tag="VF226_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7073725.1"
                     /translation="MALGGGGARGLAHILMLEVFDEFGIKPTVIAGTSIGALYGAAYA
                     SGLSAAQLHAYTEEILGRRFDLIRQLFAARSEPVQRLLRVLPVRSSLINSATLLDLLF
                     PDHVAKDFSELKIPLKVVATDLSSQEAVVLDEGPLRPAVAASIAIPVLFSPVVIGNRI
                     LVDGGLANPLPFDLIDGAADITVAIDVNAKTSQADIGPRPTAVEVIVQSIQILQKSVT
                     RERLRYRQPDILIQVAIDQFNVLEFTRPHEILQAAQPAKAALRQELLRMFGSKSGGGA
                     RAVEPRPEPHVPATSE"
     gene            complement(13410..13877)
                     /locus_tag="VF226_06755"
     CDS             complement(13410..13877)
                     /locus_tag="VF226_06755"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX7073726.1"
                     /translation="MAPRSSGSHMALALEEARRAADRGEVPVGAVIVAPDGMVVARAG
                     NRTRELADPTAHAEMLVIREACRSIGSERLTGFSLYVTLEPCPMCATAISFARIRRLY
                     YGASDPKGGGVESGPRIFTQPTCHHVPEVYAGIGEAEASRLLKAFFEARRGPD"
     gene            14092..14796
                     /locus_tag="VF226_06760"
                     /pseudo
     CDS             14092..14796
                     /locus_tag="VF226_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            14913..15494
                     /gene="rsmD"
                     /locus_tag="VF226_06765"
     CDS             14913..15494
                     /gene="rsmD"
                     /locus_tag="VF226_06765"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7073727.1"
                     /translation="MRVVGGRFRGRPLASPKDPGDQRLRPTSDRVREAVFNILTHGIP
                     EFSLVGAKVIDLFAGTGALGVEALSRGASFCLFVEQDAEGRGLIRRNIEAFGLAGITR
                     IFRRDATDLGPAGSREKFTLLFADPPYGRGLAEKALAAAAAGGWLAEGAIAVIEEATR
                     SELTLPSEFTEIDRRVWGSTQVVFARYTPKSEA"
     gene            15491..16483
                     /locus_tag="VF226_06770"
     CDS             15491..16483
                     /locus_tag="VF226_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073728.1"
                     /translation="MNERSPVQMSKPQQTRPAENVSGWIEAGLYVFAVAVLSVMNAFA
                     VAHGAHPIVFILYSLLIAAAGMLAITGLGDEPVRIMLSPMSWLVGIGAILVEVGFCLA
                     LTSLSPAEGSLSLRLSIPMAIVLGWMTFRRSPGVGVWLGAAIILLGVGTLVVIVDLGT
                     QAVGLLYGTIAAAAICLRSFAAEFHPWNRAARSVFEKMRVTGLVVLVTGIAGLLLVGL
                     AAALVGGGLIDRSDLAPAPSDLSHWPTMVMALFLGGIVFTSMNYLQFSSVVKIQTENF
                     IATSAFMPLATLLLQSVAVALGIIEAAVFDWRLLPGILLAMAGVTVLIAYGRKR"
     gene            complement(16486..18309)
                     /gene="mutL"
                     /locus_tag="VF226_06775"
     CDS             complement(16486..18309)
                     /gene="mutL"
                     /locus_tag="VF226_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX7073729.1"
                     /translation="MAIRQLSPDTINRIAAGEVIERPASVVKELVENAIDAGASEIEI
                     VTSGGGVSLIRVTDDGAGMDAADLALSVERHATSKLNGDDLLDIHTLGFRGEALPSIG
                     SVAELTIVSRTRDSDEAFSLRVERGQKGPLRPAAGNPGTRVEVRDLFSATPARLKFLK
                     SERAENMAIADVVKRLAMAHPEVGFVLVTGERTPLRLPARPPTPEGLLDRLGQVMGRM
                     FVDDALEIAGEKEGIRLSGFAGLPTLHRPDASQQFLFVNGRPVRDKLLVGAVRAAYAD
                     LIPRGRQPLLAIFVELDPREVDVNVHPAKAEVRFRNSAAVRALIVGALGHSLRQAGHR
                     ASASGGAAALDVIAKRSMPSRERPPSSAYARPASGYGAPRSFTPAIASGFAEAMQAPL
                     DAMDQPSAQGFDQSTTAATDLLDHPLGAARCQIHETYIIAQTRNSMIIVDQHAAHERL
                     VYERMKAMLADGGVARQGLLIPEIVEVGEDEAAALAECADELSDLGLVLERFGPNAVV
                     VRETPALLGQTDVQGLVRDLAADILRDGERLALKERLEAVCATMACHGSVRAGRRLTA
                     AEMNALLREMEATPLSGQCNHGRPTYVELALEDIEKLFGRR"
     gene            complement(18334..19701)
                     /locus_tag="VF226_06780"
     CDS             complement(18334..19701)
                     /locus_tag="VF226_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7073730.1"
                     /translation="MVGSARASSTTDLLPYRQIIDGVLDQAIGSHGLTRADLQSWLEK
                     LKPGLEGLRQDWETGSLPLLRVPWEMSDIAAAEEAMARLSVGAEVIVFFGTGGSSLGG
                     QTLAQLGGWNIPGTADEAQKMRPRTRFYDNLDPDTLARSLASLDLAKTRFVVISKSGN
                     TPETLVQAIAALDADKAAGLTNDIPRLFLGLTEPAVPGRKNGLRQLFEAHGIPLLDHH
                     TGIGGRYSVLTNVGLLPAIARGLDVRSLRAGAAQVVEQLRAAAHPADVPAAVGAAVNI
                     ALAKERGVRISVMMPYADRLARFAHWYVQLWAESLGKNGMGTTPIACLGPLDQHSQLQ
                     LFMDGPREHLITVLRPAAAGTGPRIDAELAAKAGLDEMAGRHAGDLVAAQTHGVPRAL
                     TQAGRPVRIFDIDRLDERTLGALLMHFMLETILAGRLLGVDPFDQPAVELGKKLARER
                     LQQGS"
     gene            complement(19798..21171)
                     /locus_tag="VF226_06785"
     CDS             complement(19798..21171)
                     /locus_tag="VF226_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7073731.1"
                     /translation="MGGKRRQGLSHTLSTHLTVLALVSALLFGAAAMHATSASAMEIK
                     TVKSPGGIEAWLVEEHSVPLMAMRFAFEGGNSQDPDGKEGVANFLTAMMDEGAGDLTA
                     IEFQERMEEFAVRMAFEDSRDSLYGSFETLTANRDEAVELLRLAVTKPRFDADAVERI
                     RGQLLTSLAFAAKDPDRVAAREWFAAAFPGHPYGRPATGTPESVAKITREDLKTYHKR
                     VFAKDTLKVAVVGDIDAETLGKLLDKVFGDLPDKAELEPVPFIEPKKGGNLRVVEMPV
                     PQSVATFGLAGIPRKDPDFIPAFVMNHILGGGGFSSRLMEEVREKRGLAYSVYSYLQP
                     YQHAAIFAGGVATKNEEIGQSLKVIRAELERMATDGPTPEELESAKSYLIGSYPLRFD
                     TNAKIANQLLGIMQDGLGKDYVDKRNALIEAVTLEDVKRAAKRILSTDNLIMTVVGQP
                     KNLKERI"
     gene            complement(21186..22589)
                     /locus_tag="VF226_06790"
     CDS             complement(21186..22589)
                     /locus_tag="VF226_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7073732.1"
                     /translation="MLTRVRVSLSTLLAFTMMLAFAHDPLAAQTTERRASEFTLSNGM
                     QVVVVPDHRAPVVTHMVWYRVGAADEPPGVSGIAHFLEHLMFKSTEKIASGEFSRIVS
                     RMGGQDNAFTSQDATAYFQRIHKDRLGQVMQMEADRMVNLRLTEQEVLTERDVILEER
                     RSRIENNPQAVLDEQMNAVLYYSHPYGIPVIGWEHEIARLTREDALAFYKRHYAPNNA
                     ILVVSGDVTPEEVRRLAEESYGKIPANPEIMPRKRPMDPEQRAARRVEIKDPRAGKPS
                     VQRYYVTPSYVTAKPGEAEALDLLMKIAGSGATSRLYQKLVATDKIASSAGGWYGGSS
                     MDFGKIAVYAIPVDGVSLDQVEQAIDEVLADIIENGVTEAELERAKKAYLAEYIYESD
                     NQATLARRYGWALVVGRTVEDIESWPEKIAKVTLDDVKKVAARYLDPRRSVTGRLLPA
                     PPDVVQQTGASAPPSRS"
     gene            complement(22868..23320)
                     /locus_tag="VF226_06795"
     CDS             complement(22868..23320)
                     /locus_tag="VF226_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073733.1"
                     /translation="MNRTDRQKRIALAASTVFASLVLSGCGFGDVEFNGAVFEAMGLT
                     NSGKSHEEPKLPPRAGIVLPPDTSKLPEPGSTEVAAPPPGEAWPVDPEEREIAAAEEL
                     ERKHQEFCREALWRARAAGQTDAQITGPKGSCNPSILKALTGKDITTR"
     gene            complement(23370..23888)
                     /gene="lspA"
                     /locus_tag="VF226_06800"
     CDS             complement(23370..23888)
                     /gene="lspA"
                     /locus_tag="VF226_06800"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7073734.1"
                     /translation="MPSVAKPGWLWGPLSRLGLSVALVTLVVDQAHKWWMLAIFGIEE
                     KGRVPVLPFLDLVYVKNLGISYGLGAGVIGQGALAAFALVAALGLMIWLARGEMNRLM
                     AISIGLLIGGAVGNAIDRLHLGGVADFFLLHAYGWSWYVFNIADAAIVAGVAGLLYES
                     LRSSRNRAANGS"
     gene            complement(23894..26908)
                     /gene="ileS"
                     /locus_tag="VF226_06805"
     CDS             complement(23894..26908)
                     /gene="ileS"
                     /locus_tag="VF226_06805"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7073735.1"
                     /translation="MPKDNGKPGPQGRDWSKTLFLPRTAFPMKAGLPELEPRLIQRWS
                     ELGMYRRLREAAKGRKKFILHDGPPYANGHIHIGTGLNKILKDIVVRSQQMMGRDANY
                     VPGWDCHGLPIEWKIEEEYRAKGKTKDSVPIIELRRDCRVFAEKWINVQREEFKRLGV
                     EGDWDHPYATMSFDAEAKIAGELIKFAMNGSLYRGSKPVMWSVVERTALAEAEVEYHE
                     VTSPTIWVRFPVAEVKVTRRQLSKGLAEIDNVSQSAVVIWTTTPWTIPGNRAISYNRN
                     IQYGLYEITDAPADNWAKPGERLILADKLADSVRLAARINGWRRLSDVSEATLEAMVC
                     HHPFHGQGYEFQVRLYHGDHVTDDAGTGFVHTAPGHGAEDYAVYEEHRASFAEAGIPE
                     VPHTVGEDGYYFRHVPLFGGEHPKRVIDDKGKFGDANEAVIQELIKAGALIARGRLKH
                     SYPHSWRSKAPVIFRNTPQWFIAIDKPITIPGKKGEATIREYALKAINETRFVPAAGQ
                     NRLRGMIEQRPDWVISRQRAWGVPITVFVNKTTQQYIPNREWKRSRELTKRIVEAFKA
                     EGADAWFVDGAKERFLAGLVDDINEWEQVRDILDVWFDSGSTHAFVLEERKDLAWPAS
                     LYLEGSDQHRGWFHSSLLESCGTRGRAPYEGVLTHGFVVDEEGRKMSKSLGNVVAPQD
                     VIRQSGAEILRMWVASADYSEDLRIGPEILKSNIEAYRKIRNTLRWLLGNLAHYEEKL
                     KVRPREMPGLDRYILHRLAELDALVRQSYENYDFKRVSHALSNFCINDLSAFYFDIRK
                     DALYCEPYASKKRRACLTVLNETFNCLTIWLAPILAFTAEEAWLERYPSEDGSVHLQV
                     FPKIPKPWRDDALAKKWEQIRRVRRVVTGALEIERAAKRIGSSLEAAPDIYVADEDLL
                     DALEDVDMAEVSITSAAQVVPSEPPVSAFTLPEVPGVGVVPRRAEGRKCARSWRIVPD
                     VGSDPEYPELSARDAAAVRDFEKRNIAAAAAE"
     gene            complement(26996..27964)
                     /locus_tag="VF226_06810"
     CDS             complement(26996..27964)
                     /locus_tag="VF226_06810"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7073736.1"
                     /translation="MRIVHGYRQVPPEIKGAALAIGNFDGVHRGHQTLLLTALKQARA
                     TGKPAGVMVFEPHPREFFRPDEPHFTLTTLQEKVRLIERLGLDFVIVLTFDASLAGLP
                     AARFIDEVLVGALQVSHVVIGYDFFFGKGRGGNPEVMRAAGREHGFGVTVIPPVAEAG
                     EVFSSSGIRAKLSAGDVRGAALDLGHWWRVAGRVTGGAKRGTGLGFPTANIVLPKGTA
                     LAHGIYAVRVYIAGERYSGAAYLGTRPTFDHGAPVLEVFLFDFEDDLYGKEIEVEFLD
                     FIRGDQRFPDAQALIAQMQADCARAREIIAAVDRQSPLAGFPLAEG"
     gene            complement(28090..28527)
                     /locus_tag="VF226_06815"
     CDS             complement(28090..28527)
                     /locus_tag="VF226_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7073737.1"
                     /translation="MTVLTRKTYYFEDLELGMEASFQRLVTEADINRFAEVTGDRNPV
                     HLDADYAAKTMFGERIAHGMLTASYISAAFGTKMPGPGVIYISQTLNFRAPVKIGQTV
                     TAKVKVVELFPAKHRARFECVCSVDGKTVLEGEAMLMVPTRPA"
     gene            28808..29581
                     /locus_tag="VF226_06820"
     CDS             28808..29581
                     /locus_tag="VF226_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEX7073738.1"
                     /translation="MSDRRPLMPKSTAVWLVENTSLTFEQIAEFCGMHLLEVKGIADG
                     DVAAGIKGMDPITTGQLTREEIKRGEQDPNYRLKLAEPKIEIPQVKTKRGPRYTPVSR
                     RHDRPNAVLWLLRNHPELKDSQIMRLVGTTKPTIQQIRDRTHWNSANLVPQDPVTLGL
                     CSQTDLDAEVEKAARRLEKERKMEPAETGGTLLPVSETVKPAEILHEEPEAPHEETPE
                     EEEARMLARLREMGSGSAQNGDEDNEEVPEGERAEEPNT"
     gene            complement(29755..29997)
                     /locus_tag="VF226_06825"
     CDS             complement(29755..29997)
                     /locus_tag="VF226_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7073739.1"
                     /translation="MVSPTRYDHPEAAAQGDRSCRIDLNTAPEDVLAAVSAVGPARIQ
                     ALVYRRPFRSWDDVARVPGISQTIIERLKSSGAGLS"
     gene            complement(30115..31425)
                     /locus_tag="VF226_06830"
     CDS             complement(30115..31425)
                     /locus_tag="VF226_06830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7073740.1"
                     /translation="MPSARDARLLSAARKLVELIAERLDIDASVRLWDGSLVPLGEAV
                     GGPFTISIAGPGVIGSLLRRPTIDNLIRHYAEGRIDFEGGTLIDFGRQLNRNGRSASL
                     KGMNKLVLLKTLAPFLLVPADRTADVHGFSGDITGRSQRRRDNKAFVQFHYDLSNEFY
                     ALFLDPEMVYSCAYFTDWGNDLATAQRDKLDMICRKLRLKPGERLLDIGCGWGGLVCH
                     AARHYGVRAHGITLSEKQLGYAREKVKRLGLEGQVTLDLQDYSSVRGTFDKIASVGMY
                     EHIGLANIPRYMAKVQSLLAPDGLFLNHAISRRAPRPSWRGRRMRPEQRAIAKYIFPG
                     GELDDIGHSIVAMERAGFEVHDVEAWRLHYARTCQLWCERLTANRERAIAFVGEEKYR
                     IWVAYLAGVSLAFSRGTARIYQTLASKSAKRPPKLPPTRADLYH"
     gene            complement(31539..31979)
                     /locus_tag="VF226_06835"
     CDS             complement(31539..31979)
                     /locus_tag="VF226_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073741.1"
                     /translation="MATDSRDNGFLLSRRDCTKFCLWMMLLALSGRPCTPSAMAASTA
                     PPGTASGVQLIMIEDRGCPYCARWNKEVGVAYAASAEGHFAPLVRRPRNHPDVKKFAK
                     VIYSPTFIVVRDGVELGRIVGYPGADFFWGMLDQILTAAGFKSG"
     gene            32163..32660
                     /gene="soxY"
                     /locus_tag="VF226_06840"
     CDS             32163..32660
                     /gene="soxY"
                     /locus_tag="VF226_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX7073742.1"
                     /translation="MAAPLETDVKNSLLRAAISRRGLLVGVAGATTALLLSDSDALAA
                     ADDGWEAALKKIIGEATPVNDKIKLEMPEIAENGNTVPFSVSVDSPMTTEDYVKSVHV
                     ISTGNPQPVIATFHFTPESGKAAVASRMRLAKTQDIISVAELSDGKFAMTKKTVKVTI
                     GGCGG"
     gene            32712..>32944
                     /gene="soxZ"
                     /locus_tag="VF226_06845"
     CDS             32712..>32944
                     /gene="soxZ"
                     /locus_tag="VF226_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX7073743.1"
                     /translation="MATKPRIRVPETVKAGEVIEVKTLISHIMETGQRKDQDGKPIPR
                     NIINTFTATFNGKEVFKVDLHPGISANPYMAFHV"
BASE COUNT         6037 a        10700 c        10446 g         5761 t
ORIGIN      
        1 ggctcgtgcg tctccgctct gctcggcggc atcggcgcca actggcccgc gccgcccgcg
       61 gactggatca acgccgtcaa ggcgaagaaa gaggaaaaca tcgccaaaat ggcgccgaag
      121 ctgatgaaga acacggcgcc catggatttc cacagcgcgc tcggcgcgct gcgcaacgtg
      181 atcaaggagc gccccgatgc gatcctcgtc aacgagggcg ccaacacgct cgacctcgcg
      241 cgcggcatca tcgacatgta ccagccgcgc aaacgcctgg acgtgggcac ctggggcgtc
      301 atgggcatcg gcatgggctt cgccatcgct gccgccatcg agaccggcaa gcccgtgctt
      361 gcggtggaag gcgacagcgc tttcggcttc tccggcatgg aggtcgaaac gatctgccgc
      421 tacaagctgc cggtctgcgt cgtggtcttc aacaatgacg gcatctaccg cggtgtcgac
      481 gtcaatccga ccggtggccc cgacccttcg ccgatggtct tcgtcaagga cgcccggtac
      541 gacaagatca tggaagcgtt cggcggcgca ggcgcccacg tgacgacgcc cgacgagctc
      601 taccgcgccg tcagcgccgc catggattca ggcaaaccaa ccctcatcaa tgcagtgatc
      661 gacccggcgg cgggtaccga aagcggccgc atcggtaacc tcaacccgca gagcgtggtc
      721 aagaagaagt aagggaggca tacaaatgaa agctcttgaa ggggtgcgta tcctcgactt
      781 cacgcacgtt cagtccggtc cgacctgcac gcagcttctg gcctggttcg gcgcagatgt
      841 gatcaaggtc gagcgccccg gcgtcggcga catcacgcgc ggccagctcc aggacctccc
      901 gaaagtggac agcctctact tcacgatgct gaaccacaac aagcggtcga tcacgctcga
      961 cacaaagcat cccaagggca aggaggtgct ggagcgcctc atcaaggcat gcgacgtgct
     1021 ggtcgagaat ttcgcacccg gcgtgctcga ccgcatgggc ttttcctggg agcgcatcca
     1081 ggagctcaat ccgcggatga tctacgcctc gatcaagggt ttcggacccg gcccatacga
     1141 ggactgcaag gtctacgaga acgtcgccca gtgcaccggc ggcgcagcct cgaccaccgg
     1201 cttcgacgat ggcccgccgc tcgtcaccgg cgcacagatc ggcgacagcg gcaccggcct
     1261 gcatctcgcg cttggtatcg tcacggcgct ttatcaccgt acccactcgg ggcgcggcca
     1321 gcgcgtcacc tgtgcgatgc aggacggagt gctcaatctt tgccgcgtga agctgcgtga
     1381 ccagcagcga ctggcacatg gtccactgac ggagtacccg cagtacagcg agggcaagcc
     1441 gttcggtgat gcagtgccgc gttctggcaa cgcctcgggc ggtggacagc cgggctggat
     1501 tctgaaatgc aaaggctggg agaccgatcc caacgcctac atctacttca ttacgcaggc
     1561 gcccgtctgg gacaagatct gcgacgtgat cggcgagccc gactggaaga cgcaccccga
     1621 tttcgcgaca ccccaggcgc gactgccgcg cctcaagcag gtgttcgatc ggattgagca
     1681 gtggaccatg accaagacga agttcgaggc catggaaatc ctcaaccggt atgacatccc
     1741 ttgcggtccg atcctgtcga tgaaggagct ggcagaggat cagtcgttgc gcgcgaccgg
     1801 cacgatcgtc gaggtcgacc atccgacccg cggcaagtac ctcaccgtgg ggaacccgat
     1861 caagctgtcg gatagccccg cggacgtgaa gcgctcgcct ctcctcggcg agcacacgga
     1921 ggaaatcctg cgcgatgtcc tcggctacag cgaggaggaa attgccgaga tcagggaagc
     1981 caccgagccc ccgcagaaac gggccgcggc ggagtagtcc ttgtgctgag agcgaatcca
     2041 gctagcgtca tccccgcgaa ggcggggatc cacgcaagct ggtcgtaatt tcgatctgtc
     2101 cgtttgatgc tgaccccagg tcatcctaaa caaccctgcg tctacatcct cgcaagcagc
     2161 cgaaatggta ttctttacgt gggcgtgaca agcaatctct tcggacgcgt aagtctgcac
     2221 aagcaagacc tgattgataa cgaagaggta cggcgtccac acgctcgtct atttcgagat
     2281 gcacacgacc atggcggaag cgattcggcg ggagaagcag cttaaaaagt ggaaccgcgc
     2341 ctggaaggtc cgcctcatcg aacaggttaa tcctgaatgg actgatcttt ggaatgaaac
     2401 gggcgagatt cgaatgagat caacaggcgg ccaggagcct cctgccgaca atttggagca
     2461 aatataggaa tctggcgtgg atgcccgcct tcgcgggcat gacgactctg ttcttgcgac
     2521 gatgccgtct aaaaaggatt gtcctcgcaa gacggagatc cacgtaggta agaggtggat
     2581 cggaataatc gatcgggaag gaatcacatg cgcatcgtag ttcacgggca ggaggccttc
     2641 ggcaaggcgg ttctggagag gcttctcgaa cgcggcgaga atgtcgttgc cgtgttctgc
     2701 gctccggaca aggagggcaa ggcgcgcgac ccgctcgcag tttttgccga ggagaagggg
     2761 cttcctgtcc accagccgaa gtcctggaag acgcccgaag cactcgaatt gatgcgctcc
     2821 ttcgaacccg acctgtgcgt gatggcctac gtgacgctgt tcgtgccgca gccggtgctc
     2881 gatgcaccgc ggctcggaac gatccagtat cacccgtcgc ttctgccgct gcatcgcggc
     2941 ccgtcctcga taaactggcc catcgccatg gggcgcacgc gcaccgggct cacgattttc
     3001 tggccggacg aaggtctcga cgaaggcccg atcctcctcc agaaggaagt cgatatcggc
     3061 cctgacgaaa cattgggcga tgtctacttc aagaagctct tcccgctcgg cgtcgatgcc
     3121 atgatggagg ccgtggacct cgtgcgcgag ggcaaggcgc cgaagatccc gcaggatcat
     3181 tcgaaagcga cctacgagag ctggttcaag aaggatctgg ccgagatcga ctggtcgaag
     3241 cctgtttccg aggtctacaa cctgatccgc gccaccaacc cggcccctgg cgcgtggagc
     3301 atcctcaaag gcacccggat cgacatcttc gattgcgcca agctcgacgg ctcgggaaat
     3361 cccggcgaga tcatggccat taccggcgaa ggcattcagg tcgccgcaaa aggtggccgc
     3421 attctcgtca aacgagttcg tgctgccggc ggtcagaaga tcccggcaac cgaatatgcg
     3481 gtgtccgccg gcctcaaggt cggagaccgg ttcgatccgc catcgcccaa gcaaacaacc
     3541 acatccaaga aacagtcagg aggagcgacg cccgtggcct tcaaatcgga tatcgaaata
     3601 tctcgcgagg ccaaaatgaa gccgatcgca gaggtcggca aaaaggtcgg catccctccc
     3661 gaggcgctgc tgaactacgg ccccaccaag gccaaggtct ccttcgattt catcaaaacg
     3721 ctcgaagacc gaaaggacgg caagctgatc cttgtatcgg cgatcaaccc gaccccggcc
     3781 ggcgagggta agaccacgac cacggtcggt ctcggcgacg gactgaaccg gatcggcgcc
     3841 aaggccatgt gctgcctgcg ggagccatcg ctcggcccct gcttcggcat gaagggcggc
     3901 gcggccggtg gcggatacgc ccaggtcgtg ccgatggagg acatcaacct tcatttcacg
     3961 ggcgacttcc atgccatcac ttcggcgaac aatctgctcg ccgcgctgat cgacaatcac
     4021 atctattggg gcaacagcct cggcatcgat ccgcggcgca tctcctggcg ccgtgtcatg
     4081 gacatgaacg accgcgcgct gcgctccatc atcaactcgc ttggcggcgt gtcgaacggc
     4141 ttcccgcggc aggacggctt cgacattacg gtcgcctctg aggtcatggc gatcctctgc
     4201 ctggccaccg acctgcagga tctcaggcgc cggctcggca acatcgtctt cggcacgacg
     4261 cgggacagaa agccgctctt cgcccgcgat ctcaaggctg acggtccgat gactgtcctg
     4321 ctcaaggacg cactgatgcc gaacctcgtg cagacgctgg agaacaaccc cgtgttcgtc
     4381 cacggtggtc cgttcgccaa catcgcccac ggctgcaact cggttctggc gacgaaaacg
     4441 gccttgaagc tcgccgacta tgttgtgaca gaagccggtt tcggtgccga cctcggcgcg
     4501 gagaagttct tcgacatcaa gtgccgcaaa gccggcctca agccgtcggc gaccgtgatc
     4561 gtcgccaccg tccgggcgct caagatgcac ggcggcatcg ccaaggaaga cctcaagggc
     4621 gagaacgtgg aggcggtccg caagggcgcg gagaacctca agcgtcatct ggagaacgtg
     4681 gccaagttcg gcattccgcc cgttgtggcc atcaaccgct tcgtcacgga taccgatgcc
     4741 gagatcgcgg cggtcaagga gctcgccgag agcatgggga cacgcgcctt cctgtgcacc
     4801 cattgggccg acggcggcaa gggcacagaa gagctggccc ggtatgtggt cgagctgtgc
     4861 gacagcggaa aggcgaactt caagccgctc tacccggatg agatgccgct gtgggacaag
     4921 atcaggacga tcgccacgga gctctatggc gcgtctgata tcagcgccga cgccactgtc
     4981 cgcaaccagc tcaaggactt cgaggccaac ggctacggtc acctgccgat ctgcatggcc
     5041 aagacgcagt actcgttctc gaccaacccc aacctgcgcg gcgcaccgac gggccacgtc
     5101 gtcaacatcc gcgaggtgat cctctccgca ggcgccgagt tcgtcgttgc ggtttgcggc
     5161 gatctcatgc gcatgcccgg cctgccgcgc gtgccctccg cagagcagat gtacgtcaac
     5221 gagcagggct acatcgagaa cctgtcctga cgcgggcctc aagccttctc cctgcaatgc
     5281 ggggagaagg cctgcattcc cgaggggacc ggctctcgtc tacagcgtcc cggggaaggc
     5341 gccgccgtcg atcacgatat tctggccggt gatgtagccg gcgtgggcgg agcagagaaa
     5401 agccgcagtc gcgccgaact cttccggagt gccgaagcgc tttgccggaa cctcgttctt
     5461 gcgcgcctcg tgcaccacct catacggctg accgcgcgcc tccgcgatct tccgcgtagt
     5521 ggccctgagg cggtccgttt cgaaaggtcc aggcagcagc gcgttgatcg tgacgttccg
     5581 atgcaccgtc ttgcgggcga gcgccgcgac cgcgccggtc aggccggagc gggcaccgtt
     5641 cgataattcg agcgtcggaa tgggtgcttt cacagacgag ctggtgatgt tcacgatgcg
     5701 tccgaagccg cgctccatca tgccgtagac ggtcgcgtga atgagcgcga tcggcgtcag
     5761 catgttgcct tcgacagctt tgcgccaatc atcgagcgag aagttcttga agtcgccggg
     5821 aggcggcccg ccggcgttgt tgatcagaat atccgggttg ggacaggcgg cgagcagcgc
     5881 ccggcggcct tcctccgtcg tcacgtcgca gacgacggtc gtaacgcttg ccgcacccag
     5941 cccgcgcaga tgctccgccg tcgcctcaag tgcctccttg ccgcgcgcgc aaatggtcag
     6001 cgccacgcct tcgcgcgcca acgcctcggc gcagcccctg cccaatccct tagaagcggc
     6061 acaaacgatt gccgacctgc ctctgatgcc aagatccatg gcttccgtat tcctccgttg
     6121 tcgatggggg aagactatgc gcgccctcgt atgatgtcga ccgctcgcct acagccagcc
     6181 cttgcgccgg aaatagacga acggcagcac ggcagagagc accatcagca agagcgccag
     6241 cggatagccg aaagtccagt cgagctccgg catggtgcgg aagttcatgc cgtagatgga
     6301 agccaccagc gtcggcggaa gcagcacgac ggccacgacc gaaaagagct tgatgatctg
     6361 gttttgctcg atcgagacga gccccaacga ggcatcgagc atgaaagtga tccgcgcatt
     6421 aagatagttg aggtgatcgg cgagcgattg tacgtcccgg cttgccgtcc tgatctgcgc
     6481 gcgtacacgt tcggcctcct tgcgcctcag ggctacctga ctgaggtagg tcagcaagcg
     6541 accgagcgag agcaggctgt ctcgcatctt cgacgcaatc tctccttcgc ggccaatctg
     6601 cttcaggatc acctcgaaac gtcgcgtccg cgtcgccgat ccgcctttca tatcgaagat
     6661 cgtctgcgcc aagcggtccg catcggcctg caggcgttcg atgatgtcgg cctcacggtc
     6721 gatgatcgtt tcaagcaagc ccagcaatat cgccattgcg gaatcgattg gcgtgtcgcc
     6781 cttctgcgcc ctggcgagaa aaatcgagaa ggcgcgcggc tcggcataac gcacggtcac
     6841 gagtttgctc tcggtgagaa tgaaggtgat cgctgtcgac tctggactca ccgtttccgc
     6901 ccgatgcagc accgtagccg tcatgacgta agcgccggat tcgtgatata agcggctcga
     6961 gacctcgatt tcggccatct cctcgcgggt cggcacggcg atgccaagct cccgctcgac
     7021 gagtgcctcc tccgctggga ttggattgag gaggtcgatc cagacggcct cagcggcgga
     7081 aagggcccct tgggctttca caaggccagc cccgtgtgcg tgatagatgg tcagcatgcc
     7141 agcgccttcc ctccgcctgt tccgagctgg aggcagtgat acaccaatgc gcacggcgtg
     7201 gcaggcagcc aaagccgagc tttctgcgtt ctgaactcag tcagagtacg ggtccggtcc
     7261 gatgaaggac gtagtagagc tgaaccggaa atacggtgtc gataacgagc tcggcttttg
     7321 cgacataaga ggcgagccgt tggcctggat cagaaacgac gcctgcgcgg ccacgatcgc
     7381 gctcaatggt gcacatctgg taagctggta tccctacggc cacgacgagg tcttctgggt
     7441 gtcctctgtc cggccctcgt cctcaacgat gtcccaacgc gggggagtgc ctgtctgctg
     7501 gccatggttc ggcccccatc cgacggatcc tacgaaacca caacatggat tcgtgcgaaa
     7561 ccgccggtgg acggtggtgt cggccaacag cacgtcgcaa gtcacagagc tgacgctcag
     7621 gaccgagacg acgcccgagg atgcggcgct gtggccccat cacgccgaag taagcctgcg
     7681 gttcatcgcc ggggcaacgc tatgtctcga gctcgcgacc cgcaacaccg gctcgcaggc
     7741 attcgagctg acacaagcgc tacactcgta tttcaaggtc tcggatattg ccaatgtggc
     7801 gatcgaaggc tttgacggcc tcgagtatct cgacaaggtc gacgcctacg cccgcaagcg
     7861 gcaatccggc cagatcacca ttaccggtga gaccgaccgc atctatctcg gccataccgg
     7921 acccgccatc attcacgatt ccggactcgg gcgagggatc atcgtgacca agtccggcag
     7981 caccagcagt gtcgtctgga atccatggga ggagcgggcg agacagatgg gcgatctcgg
     8041 cgaggacggc taccggcgga tggtttgcgt cgaaacggcg aatgcaggtg atgatgtcgt
     8101 gcgcatcgat ccgggccatc agcacactct cgtcgcaaat attcagattg cacgcggctg
     8161 agggctcatt gcactgcgac gcacggactc ttgcagagca ccatgccgcc cctcatattg
     8221 gacgccaggt ttcaacagag gaaaggaggt gatccaatgt cgagtgagat gtggactctg
     8281 ccgtttgggg acgtgttcgc agcgaggacg ttggagcagc ctccgcgttt ttcctgagac
     8341 gccgtgagcc ggcgtattcg cccggccagt gccaacggca gccttcactc acgaaaggcc
     8401 acctgatcgg gtggcctttt tcgtttggct ccagcccccc ggcgggcggg caagcgaacc
     8461 agcgtcgggc tgctgaaccg ctgatgaacg acccgctccg atgtcgttca tgtctgccgt
     8521 gccagtgtca ggccatcgga acgacccgtt ccgacgcgat ctgggcaaca ctggcatgga
     8581 gatattcgaa gatgatgctg aagtcagcca tattcgcgac tgtcctggca gccgccgccg
     8641 cgaccggcgc ggccagcacg gcttctgctg caccggctgc cgcgcctgca gtgctcggcg
     8701 gcattcaaaa cacctctgat gccgttcagc aggtccactg gcgccgtacc cgcgtcgtga
     8761 cccgatgcat cggcggacgc cgcgtactga tgcgtatcaa ctggcgcggc agggttgttt
     8821 cacgccgcgt gatcgggcgc tgctggcacc accaccgcaa gcatcgccac cgctggtgat
     8881 cgccggcgag ccgcacggcc gatcacgtct gcaaacgaca aggccccgat cgcgtgcctc
     8941 cggcgatcgg ggcgttgtcg tttgcgcccg atttgggatg tcagtcttgc ttcgacttgc
     9001 cggaagcacc tgaccgccgg gcccgaagct cacgatagcg ctggagcgaa gcggccggcg
     9061 tcgcataggc ctcctgcagc tcgacgctcc agtaccgcaa ctcctgcaga ggaatttgct
     9121 cgcccgtgac ggcgcagcgg acgtattcgc ccggcgacaa aacctggaac tcgccatcca
     9181 ggtagcggac gcgagcctcg ccgtgtgatc cgaacagttt ttcgaaacga ttcatttacc
     9241 ttctcggcgg atggcacgtt cagcaccgtt agacttcaga ttgcgaatgc ggaccggccc
     9301 gtcaaggctg ggcgacactg atacacgctc gaaatcgcac atcgttgttt cagaacagcg
     9361 agccctgtcc gccttggggt gggttgcgct tgcgccgcgg ctggcgcacc tgagccggat
     9421 cgacgatgct cgagacggtc ggccgggacg gacccctggt cgtcctgaca tcgcgcacgt
     9481 ctgcgtcgat gcagccgtcg gcaagctcca cctgaatgtc cgtgcccagg ccgaccgcgg
     9541 cgacggagcg gatcgtgtgc ccttcggcat cccgcaccag cgcaaagccg cgttccagca
     9601 caccctggta gctgagggac aagagcagct tcgacagccc cgataggcgg gctctttcgt
     9661 gcgcgacccg gctcctgaaa gcctgctccg cccgcaagcc gcagctcgcc aatctctcgc
     9721 gcccgcgatc cacccggctg cggacggcct cgatgccgag gcgcccggcc gtgcgctcga
     9781 agcgggaccg ccgcgcgccg gcaaggcgcg acagcgcatc ccggctgtgc cggctcagtg
     9841 cctcgaggcg gtcgcgacag cgattaagac gtgcttccag cagccgcggt tgcagccgcg
     9901 aagacacgcg ggcgtgccgc atcgcatgcg cgcgcgtgtt ggcgagcaga gcccggccca
     9961 accgctgctc aactgagtcg aaccgctgcc tcgggagggc cagcagatcc tcgcggcgcg
    10021 gcaggccgcg cgcagcggct ttgagatgcg ttcgagcgcc ttcgaggacg cgacgcatgc
    10081 tactggcgag gcgcaacgcg agcgtcgccg tcccctccac cagctcggaa tgcttgggca
    10141 cggcccattc cgcggccttg gtcggcgtcg gcgcacgggc atcggcggcg agatcgatga
    10201 gcgtccagtc cgtttcatgg ccgacagcgg agatgagcgg gatctcgctt tccgcggccg
    10261 cgcgcaccac gccctcgtgg ttgaagcccc ataggtcctc gaggctgccg ccaccgcgtg
    10321 caacgatcag gacatcgggc cgcggaacgg ggccacctgc aggcagggcg ttgaagccgc
    10381 ggatcgcggc ggcaatctcg gcagagcagg tttcgccctg caccctgaca ggccagacga
    10441 gcacatagac gggaaaccgc tcgtcgaagc catgcagcat gtcgcggatg acggcccccg
    10501 tcggcgaggt gatgatgccg accacgcgcg gcagaaacgg cagcggcttc ttgcggtcct
    10561 cgtcgaacag gccttcggcc gccagcttcc gcttgcgctc ctcgagcacg gccatcagcg
    10621 cgccgatgcc cgcaggctcc aggctttcga tgacgatttg gtacttggac gagttcgggt
    10681 aggtcgtgat gcgcccctgc gcgacgacct ccatgccctc ctctggcttg accttgaggc
    10741 gctgccacgt cgtgcgccag atcaccgcct cgatttttgc cttgtcgtcc ttgagcgcga
    10801 agtagcagtg gcccgaagag tgcgtgccgc ggaagcctga gatttcaccg cgcaaacgca
    10861 cgaagccgaa cccgtcctcc aacgcccgct tgatggcgct cgcgagctcg gaaacggtat
    10921 actcggcgac attggcgccg gtgcgctgct gtgtggcaga ttcggccacg atcatccctg
    10981 ctatgtggtg tctggaaccg atgatacacc atggtggccg ccatacggcg gcgtccaaat
    11041 gccgttttcc cacgagatgc cggactaggc ttgcatgaac gtactccttc tgggttccgg
    11101 cggccgtgag cacgctctgg cctgggccat agcggcaagc ccgctgctcg atacgcttta
    11161 ctgtgcgccc ggcaacgccg gcattgccga ggtcgcccga tgcgtgccgc tcgacatcac
    11221 ggatcatgcg gctgtggcgg cgttctgccg cgagaagcgg atcgacctcg tcgtcgtcgg
    11281 tcccgaggca ccgctcgtcg ccgggattgc cgatgacctt tccgcggcag ggttcaaggt
    11341 cttcgggccc tcgcgcgcgg ctgcacagct cgaaggctcg aagggcttcg cgaaggatct
    11401 ctgccgcgag ctcggtgttc caacggccgc ctacggccgg ttctccgatg ctgcgtccgc
    11461 gaaggcgtac gcagggcagc tccccctgcc cgtggtcgtc aaggccgacg ggcttgctgc
    11521 gggcaagggc gttgtgatcg ccgaaacaag ggaagccgcc gacgcggcga tagacgcctg
    11581 cctgggcggc gccttcggaa cggctggcgc cgaggtcgtc atcgaggaat acctgcgtgg
    11641 cgaggaggcg agctttttcg cgcttgtcga tggcaaacac gccctcccgc tcggcgcggc
    11701 gcaggaccac aagcgcgcgt tcgacggtga tacgggcccg aacaccggcg gtatgggggc
    11761 ctattcaccc gcaccagtga tgacgcccga catggccgag cgcgtcatgg ccgagatcat
    11821 ccgccccatc gtcgccggca tggcggctcg gggcacgccg tacaagggcg tgctgtttgc
    11881 cgggctgatg atcacgcccg agggtccgaa gctcatcgaa ttcaacgtcc gcttcggcga
    11941 tcccgagtgc caggtgctca tgatgcgcct caaatcggac ctgcttcccg cgctaatggc
    12001 gacagtcgac ggcgtgctcg gcaatttcga cctgcgctgg cacgatgaca cggcgctgac
    12061 ggttgtcatg gccgccaacg gctatcccgg cacacccgcg aagggctcgg agatccgcgg
    12121 gttggaggag gccgccaagg tgcccggcgt cgagatcttc cacgccggca cgcgccgcga
    12181 tggcacgcgc atcgtctcca atggcgggcg cgttctcaac gtcaccgcgc gcggacggac
    12241 cgtcagcgaa gcccgcgacc gcgcctatga ggcgatcagc cggatcgagt ggccggaagg
    12301 cttctgcagg agcgacatcg gctggagagc catcgcgcgc gagcgaggcg gcgaacccgc
    12361 ttgactctgc tcgcgctgca agccgcgctg ccttcgacca tgtgccgcct tgacgcgcag
    12421 ggcgctttgg cctcgttgta gctgagatgg atcagcagct tcagcaacac tccggacagc
    12481 cacgaaagaa actgccgagc gtggccttgg cactcggcgg gggaggcgca cgcgggcttg
    12541 cccacattct gatgctggaa gtcttcgacg agttcggcat caagccgacc gtgatagccg
    12601 gcacctccat cggcgctctt tatggggccg cctatgcctc agggctcagc gcagcccagc
    12661 tccacgccta taccgaagaa atactgggcc ggcgcttcga tctcatccgg cagctcttcg
    12721 cggcccgttc ggagccggtg caacggctgc tccgggtgct gccggtgcgc tcctcgctca
    12781 tcaattcggc aacgctgctc gacttgctgt tcccggacca cgtggcgaag gacttttccg
    12841 agctcaagat tccgctcaag gtcgtcgcaa cggacctttc ctcacaggag gccgtcgtgc
    12901 tggacgaagg gcccttgcgg cccgcggtag cggccagcat cgccatacct gtcctgttct
    12961 cgcccgtcgt catcggcaac cggattctgg tcgatggtgg gctcgcaaat cccctgccgt
    13021 tcgacctcat agacggagcg gccgatataa ccgtcgccat cgacgtcaac gccaagacgt
    13081 cccaagctga tatcggcccg cgaccgaccg cggtcgaggt gatcgtacag tcgatccaga
    13141 tcctgcagaa gtctgtcacc cgcgagcggc tccggtatcg gcagcctgat atcctgatcc
    13201 aggtcgcaat cgatcagttc aacgtcctcg aattcacaag gccgcacgaa atactccagg
    13261 ctgcacagcc ggcgaaggcg gcgctcaggc aggagctttt gcgcatgttt ggctcaaaga
    13321 gcggcggcgg ggctcgcgcc gtcgagccgc ggcccgagcc gcacgtgccg gcgacatccg
    13381 aataggcctt aagccgcgtc cactctcgcc tagtccggcc cgcggcgcgc ctcgaaaaat
    13441 gctttgagca gccgagatgc ctcagcctct ccgatgccgg catagacctc cggaacatga
    13501 tggcaggtcg gctgcgtgaa gatgcgcggc ccggactcga cgccgccgcc cttgggatcg
    13561 ctcgcgccgt agtagagccg ccggatgcgt gcgaaggaga tggctgttgc gcacatcggg
    13621 catggctcca gggtgacata gaggctgaag ccggtgagcc gctcggaacc tatggagcgg
    13681 caggcctccc gaatgaccag catttccgcg tgtgccgtcg ggtcggccaa ctcgcgcgta
    13741 cggttgccgg cgcgggcgac taccatgccg tcgggcgcaa cgatcaccgc acccacggga
    13801 acctcgccac gatctgccgc acgccgggct tcctccagcg cgagcgccat gtggcttcct
    13861 gaagagcgcg gtgccataaa ggcctctcct tcaaatcgga tcaactcgtt catcatgcac
    13921 aagccccgag cgggtcaacg aagtcaccgc cccgaagccg aacgcggcaa gcgcgcggag
    13981 cggcgccctt ctacggcgag gccatctgag gcgagccgcc gcaatgccgg cggccggcca
    14041 cgtccgcccg agccccagga ggccgctgcc ggccgtccgc ctgccaggat gcgcatagcc
    14101 aaggcaatgg cccgcgctgg cctgtgctcg cgccgcgagg ccgagcgctg gatcgccgag
    14161 ggccgcgtca ccgtcaacgg cgaggtgctg acaagccccg cccgcgatgt cggacccgac
    14221 gacgtcgtaa tggtcgatgg caaacccctg cccgacatgg agcccgttca ggtctggcgt
    14281 tactacaaac cgcgcggcct cgtcacgacg catcgtgacc cgcagggacg gccgaccgtc
    14341 ttcgaaaatc ttccggaggg catgccccgc gtcatctcgg tcggccgcct cgacttcaac
    14401 accgagggct tgctgttgct gaccacggac ggcgccctgg cccgtcatct ggagctgccg
    14461 tcgacgggct ggctcaggcg ctatcgcgta cgggcccacg ggagtgtcac acaggccgac
    14521 ctcgacaagt tgaaggaagg catcgagctc gacggcgtgc actacggccc catcgaggcc
    14581 acgctcgatc gtgtgcaggg cagcaacgtc tggctcacga tcgggctgag ggaaggcaag
    14641 aaccgcgaag tgcgcaaggt gctcggcacg ctgaacctgg atgtgaaccg gctcatccgc
    14701 atctccttcg ggccgtttca gcttctcgac ctgaagcctg gccaagtcga gccggtgcgc
    14761 cggcgggtgc tcagcgatca gctcggcccc cggattgcgc gcgagcttgg tctgagcgtg
    14821 gcaagcgacg aggagaaggc ccgccgcgaa cgatacaagg ccgagcgcgc caaagccgac
    14881 cggcacgggc cgaaagcgga ggacgacggg cgatgagagt tgtcggaggc cggttccgcg
    14941 gccggccgct ggcgtcgccg aaagatcccg gcgaccagcg cctgcggcca acctcggacc
    15001 gtgtgcggga ggccgtgttc aacatcctca cccacggcat cccagagttt tcgctcgtgg
    15061 gcgccaaggt gatcgacctg ttcgccggta ccggcgcgct cggcgtggag gctttgtcgc
    15121 gcggtgcgag cttctgcctc ttcgtggagc aggacgcgga aggccgcggt ctcatccgtc
    15181 gcaatatcga ggcgttcggt ctggcgggga tcacgaggat tttccgccgg gatgcgacgg
    15241 atctcggacc ggccggcagc cgcgaaaaat tcaccttgct gtttgccgac ccgccttacg
    15301 gccgcggttt ggccgagaag gcgctcgctg ccgcagcggc cggtggctgg ctcgccgagg
    15361 gagccatcgc cgtcatcgag gaagccacgc gaagcgagct tacgttgccc agcgagttca
    15421 cggaaatcga ccggcgcgtg tggggcagca cccaggtcgt gtttgcccgc tacacaccga
    15481 agtccgaagc atgaacgaaa gatcacccgt gcagatgtcc aagccgcaac agacccgtcc
    15541 ggccgagaat gtcagcggct ggatcgaggc cgggctttat gtcttcgccg ttgcggtgct
    15601 gagcgtcatg aacgcttttg ccgttgccca tggcgcgcac cccatcgtct tcatcctcta
    15661 ctcgctgctg atcgcggcgg caggcatgct tgccatcacg ggcctcggcg acgagcccgt
    15721 caggatcatg ctgtcgccca tgagctggct cgtcggaatc ggcgccattc tcgtcgaggt
    15781 cggcttttgc ctcgcgctga cgtcgctctc gccggcggaa ggaagcttgt cgttgcgtct
    15841 ttcaatccca atggcgatcg tgctcggctg gatgacgttt cgccggagcc ccggcgtcgg
    15901 agtctggctc ggcgccgcca tcatcctgct cggcgtcggt acattggtcg tgattgtcga
    15961 ccttgggacc caggccgtcg gtttgctcta cgggacaatc gctgcggcgg ccatctgctt
    16021 gcgcagtttt gcagccgaat tccacccatg gaaccgggcc gcgcggagcg ttttcgagaa
    16081 aatgcgcgtg accggtctcg tcgttctggt gaccggtatc gccggcctgc tcctcgtcgg
    16141 cttggccgca gcactcgtcg gaggcgggct catcgaccgg agcgatcttg ccccggctcc
    16201 atccgacctg tcgcactggc ccacgatggt gatggcgctg tttctgggag gcatcgtctt
    16261 cacgtcgatg aactacctgc agttctcgtc ggtcgtgaag atccagacag agaatttcat
    16321 cgctaccagc gcgttcatgc cgctggcgac gctgcttctg cagagcgtgg cagtggcact
    16381 cgggatcatc gaggccgcgg ttttcgattg gcggcttctg ccgggcattt tgctggcgat
    16441 ggcaggcgtg acggtgctga ttgcatacgg ccggaagcga tagccttagc gccggccgaa
    16501 cagcttttcg atgtcctcga gggccagctc gacgtatgtg ggccggccat gattgcactg
    16561 ccccgacagc ggcgtcgcct ccatctcgcg gagcagcgca ttcatctccg ccgcagtgag
    16621 ccgccgcccg gcgcgcacgc tgccatgaca ggccatggtg gcgcagaccg cttcgaggcg
    16681 ttccttgagg gcgagccgtt ctccgtcccg caagatgtcc gccgcgagat cgcgcaccag
    16741 tccttgcaca tcggtctggc cgagcagtgc cggcgtctct cgcacgacga cggcgttcgg
    16801 cccgaaccgc tccagaacga gccccagatc ggagagctca tccgcgcact ccgcgagggc
    16861 ggccgcctcg tcctccccga cctctacgat ctcggggatg agcagcccct ggcgcgcgac
    16921 cccaccgtcg gcgagcatcg ccttcatccg ctcatagacg aggcgctcgt gggcggcgtg
    16981 ctggtcgacg atgatcatgg agttgcgggt ctgagcgatg atgtaggtct cgtggatttg
    17041 gcaacgggcg gctcccagcg gatgatccag cagatcggtc gctgccgtcg tggactgatc
    17101 aaagccttgg gcactcggct gatccatggc gtccagcgga gcctgcatgg cttcggcaaa
    17161 gccggaagcg atggccggcg tgaagctccg gggagcgccg tatccggacg caggccgcgc
    17221 gtacgcggaa gacggggggc gctcgcgaga tggcatactg cgctttgcga tcacatcgag
    17281 ggccgcagcg ccgccggaag ccgaagcgcg gtgccccgcc tgccgcaacg aatgccccag
    17341 tgcaccgacg atcagcgcgc gaacggcagc cgagttccgg aaccgcacct ccgcctttgc
    17401 cggatgcacg ttgacgtcca cctcgcgcgg gtcgagctcg acgaaaatgg cgagcagcgg
    17461 ctgacggccg cgcggaatga ggtcggcgta ggcggcgcgc acggcaccga cgagcagctt
    17521 gtcgcgcacc ggccgcccgt tcacgaacag gaactgctgc gacgcatccg gcctgtgcag
    17581 cgtcggcagg ccagcaaagc ccgataggcg aatgccttcc ttttcgcccg cgatctccag
    17641 cgcatcgtcc acgaacatcc gtcccatcac ctggccgagg cggtccaaaa gtccctcggg
    17701 cgtgggaggc ctcgcgggca gccgcagggg cgtgcgctcc cccgtgacca gcacgaaccc
    17761 gacttccggg tgcgccatgg ccagccgctt cacgacatcg gcaatggcca tgttctcggc
    17821 acgctcggat ttcaggaatt tgagccgcgc cggggtggcc gagaacaggt cgcgcacttc
    17881 gacgcgtgtg cccggattac cggctgccgg tctgagagga cccttttggc cacgctccac
    17941 gcggagggag aaggcctcat cggagtcgcg ggtgcgcgag acgatcgtca gctcagccac
    18001 ggagccgatg gacggcagcg cctcgccacg aaatccgagg gtgtggatgt cgaggagatc
    18061 gtcgccgttc agcttcgagg tggcgtggcg ctcgaccgac agcgccagat ccgccgcatc
    18121 catgccggca ccatcgtcgg tgacgcggat gagcgacacg cccccgccgg aggtgacgat
    18181 ctcgatttcg ctggctcccg cgtcgatcgc gttctcgacc agctccttga ccacgcttgc
    18241 gggccgctcg atcacctctc ccgcggcgat gcggttgatg gtgtcgggtg atagctgccg
    18301 gatggccacg ggcccctccc cgaggtgtgc cggtcaggag ccctgctgca gccgttcgcg
    18361 ggcgagcttc ttgccgagct cgacggcagg ctggtcgaat ggatcgacgc cgagaagccg
    18421 gccggcgagg atcgtctcca acatgaagtg catcaggagt gcgccgagcg tgcgctcatc
    18481 gaggcggtct atgtcaaaga ttcgcaccgg tcggccggct tgagtcagcg ccctggggac
    18541 gccgtgcgtc tgggccgcga cgaggtcgcc ggcatggcgg cccgccattt cgtcgaggcc
    18601 agccttcgcc gcaagctcgg catcgatgcg cggacctgtc ccggccgccg ccggccgcag
    18661 cacggtgatc agatgctcgc gcgggccgtc catgaagagc tgaagctggc tgtgctgatc
    18721 gaggggcccc aggcacgcga tcggagtggt tcccatgccg ttcttgccca ggctctcggc
    18781 ccagagctga acataccagt gcgcgaagcg ggcgaggcgg tcggcgtaag gcatcatgac
    18841 gctgatacgt acgccccgct ccttggcaag tgcgatgttc accgcggcgc cgaccgcagc
    18901 gggaacgtcg gcgggatgcg cggcggcccg gagctgctcg accacctggg cggcacccgc
    18961 cctcagagag cgaacgtcga gcccgcgcgc aatggccggc agaaggccca cgttcgtcaa
    19021 aacagagtag cgtccgccaa ttccggtgtg gtgatcgagc aggggaatgc cgtgcgcctc
    19081 gaagagctgc cgcagcccgt tcttgcggcc cggcacggcc ggctccgtca gcccgaggaa
    19141 caggcgcgga atatcgttcg tcagccctgc tgccttgtcg gcgtccaacg cggcgatggc
    19201 ctgaaccagg gtctccggcg tgttgcccga cttggagata accacgaagc gtgtcttcgc
    19261 caggtccagg ctcgccaggg aacgcgccaa cgtatcggga tcgagattgt cgtagaagcg
    19321 cgtgcgcgga cgcatcttct gcgcttcatc cgccgtgccc ggaatattcc atccgccgag
    19381 ctgggcgagc gtctgaccgc cgaggctgga accgcccgta ccgaagaaga cgatcacctc
    19441 ggcgcccacc gagaggcggg ccatggcctc ctcggcagcc gcgatgtccg acatttccca
    19501 agggactcgc agcaacggca ggcttccggt ttcccagtcc tgccgcaggc cctccagccc
    19561 tggtttcagt ttctcgagcc agctctgcag gtccgcccgt gtcaggccat ggctgccgat
    19621 cgcctgatcg agaacgccgt cgatgatctg ccggtaaggg aggaggtctg ttgttgagct
    19681 ggcgcgcgca gacccgacca tgccgtcaca tctcctttta ccgctgccga tcggggacgc
    19741 agttagacct gagtcccctg cctgtgccaa gccggtagcc tggcacagga cggaacgtca
    19801 gatgcgctcc ttcaggttct tcggctggcc gacaacggtc ataatgaggt tgtcggttga
    19861 caggatgcgc tttgctgccc gcttcacgtc ttccagcgtg acggcctcga tcagcgcatt
    19921 acgcttgtcc acgtaatcct tgccaagccc gtcctgcatg atccccaaca gctggttggc
    19981 gatcttggca ttcgtatcga accgcagcgg ataggaaccg atgagatagc tcttggcact
    20041 ctcgagctcc tccggggtcg gcccatcggt ggccatgcgc tccagctcgg cacggatgac
    20101 cttgagcgat tggccgatct cctcgttttt cgtcgcgacg ccgccggcga agatggccgc
    20161 gtgctgatag ggctgcagat agctgtaaac cgaataggca agcccgcgct tctcgcgcac
    20221 ctcctccatg agccgcgagg agaacccgcc gccgcccaga atgtggttca tgacgaaggc
    20281 cggtatgaaa tccgggtcct tgcgcggaat accggcgagg ccgaacgtgg cgaccgactg
    20341 cggcacaggc atttcgacca cgcgcaggtt gccgcccttc ttgggctcga tgaacggcac
    20401 cggctcgagc tcggccttgt cgggaagatc gccgaagacc ttatccagca gcttgccgag
    20461 cgtttcggcg tcgatgtcac cgaccaccgc caccttcagc gtgtccttcg cgaagacgcg
    20521 cttgtggtag gtcttgagat cctctcgcgt gatctttgcc acgctctccg gcgtgcccgt
    20581 tgccgggcgg ccgtagggat ggcccgggaa ggcggcggca aaccactcgc gcgcggcaac
    20641 ccgatccgga tccttggcgg cgaacgcgag gctggtcagg agctggccac ggatgcgctc
    20701 caccgcgtct gcatcaaagc gcggcttggt gacggcgagc cgaagcagct cgacggcctc
    20761 gtcgcggttt gcagtcagcg tctcgaagct tccgtagagc gaatcgcgac tgtcctcgaa
    20821 ggccatgcgg acggcgaact cctccatccg ctcctggaac tcgatcgcgg tgaggtcgcc
    20881 ggctccctcg tccatcatgg ccgtgaggaa attggcgacg ccttccttgc cgtcgggatc
    20941 ctgcgagttg ccgccctcga atgcgaagcg catcgccatc agcggcacgc tatgctcctc
    21001 gaccagccac gcttcgatgc cgccagggct ttttacagtt tttatttcca ttgccgatgc
    21061 tgatgtggcg tgcatcgcgg ccgctccgaa caaaagcgcg gaaacgagcg cgagcactgt
    21121 aaggtgtgtt gaaagtgtgt gggacaagcc ctggcggcgt tttccaccca tgacctgacc
    21181 tctccttacg agcgactggg aggtgcggaa gcaccggtct gctgaacgac gtcgggagga
    21241 gcaggcagca gcctgcccgt gaccgaccgt cgcggatcga ggtagcgcgc agccaccttc
    21301 ttcacgtcgt ccagcgtcac cttggcaatc ttttccggcc aggactcgat gtcctcgacc
    21361 gtccgaccaa ccaccagcgc ccatccatag cgtcgggcca gtgtcgcctg gttatcgctc
    21421 tcgtagatgt actctgccag ataggccttc ttcgcccgct cgagctcggc ctcggtcaca
    21481 ccgttctcga tgatgtctgc gagcacttca tcgatggcct gttcgacctg gtccagcgag
    21541 acgccgtcga cgggaatggc gtagacggcg atcttgccga aatccatgct cgagccgccg
    21601 taccagccgc cggccgagga cgcgatcttg tcggtcgcga cgagcttctg atacagccgg
    21661 ctggtggcgc ccgatcctgc gatcttcatg agcaggtcga gcgcctcggc ctcgccgggc
    21721 ttggccgtga cgtagctcgg cgtcacgtaa tagcgctgca ccgaaggctt gccggcgcgc
    21781 ggatccttga tctcgacgcg gcgggcggcc cgctgctcgg gatccatcgg ccgtttgcgc
    21841 ggcatgatct cgggattggc cgggatcttg ccatagctct cctcggcaag gcggcggacc
    21901 tcctccggtg tgacatcgcc ggaaacgacg agaattgcgt tgttcggcgc gtagtgacgc
    21961 ttgtagaagg ccagcgcatc ctcgcgcgtg agccgcgcga tctcgtgctc ccagccgatc
    22021 accgggatgc cataaggatg cgagtaatag aggaccgcgt tcatctgctc gtcgagcacg
    22081 gcctgcgggt tgttttcgat ccgtgagcgg cgctcctcca ggatgacgtc gcgctcggtc
    22141 agcacctcct gctccgtgag gcgcaggttg accatgcggt ccgcctccat ctgcatcacc
    22201 tgcccgagcc gatccttgtg gatgcgctgg aagtaggccg tcgcgtcctg gctggtgaag
    22261 gcgttgtcct gcccgcccat gcgggagacg atccgcgaga actcgcccga ggcgatcttc
    22321 tcggtcgatt tgaacatcag gtgttcgagg aagtgggcga tgcccgacac cccgggaggc
    22381 tcgtcggccg cgccgacccg gtaccagacc atgtgcgtca ctaccggcgc acggtggtcc
    22441 ggaacgacaa ccacctgcat gccgttggac aaggtgaatt ccgacgcgcg ccgttcggtg
    22501 gtttgcgcgg cgagggggtc gtgtgcaaag gcaagcatca ttgtgaacgc caacagggtt
    22561 gaaagtgaga cccgaacgcg tgtgagcata ctctgtcctt tatgcaggcg cggtcccgga
    22621 attgacgaat cagcgtcaga tgatcgtgcc gggggcacca tcggcttgga tgaacgcccc
    22681 acgccgacga cattatgtcg ggttcgagcc cagagttttc aaacggagca agcagcgacg
    22741 gacatgcgac ctgtcgcgaa gcatcgcatg tcaaacaccg gccgacgaga tctgcacttg
    22801 aaatctgcgg cagccgaact gaacaatacc acgattgcgg ccattgcaag agccgcagtt
    22861 gcatgcgcta ccgcgtcgtg atgtccttgc cggtaagagc ctttaggatg gacgggttac
    22921 aggacccctt gggacccgtg atttgtgcgt cggtctgacc cgctgcgcgc gcacgccaca
    22981 gcgcctcgcg gcagaactcc tggtgtttcc gctcgagctc ctcggctgcg gcaatctcgc
    23041 gctcctcagg atctacaggc caggcttccc cggggggcgg ggcagcgacc tcggtcgagc
    23101 ctggctcggg cagcttgctg gtgtcgggcg ggagcacgat ccccgcccgg ggcggcagct
    23161 tcggctcctc gtgggacttg ccgctgttgg tgagccccat ggcctcgaac acggcaccgt
    23221 tgaactcgac gtcgccgaag ccgcaacccg acagtaccag agatgcgaag acagtgctgg
    23281 ccgccagcgc gatgcgcttc tgtcgatccg tcctgttcat gagcgccccc ggatgcagtc
    23341 gacggagaga atcgtttcct gtcggacgtt tatgaaccat ttgcggcgcg attgcgactc
    23401 gatctcagcg actcgtatag cagtccggcc acacccgcaa cgatggcggc gtcagcaata
    23461 ttgaatacgt accaggacca tccgtaggcg tgcagcagga agaaatccgc cacgccgccg
    23521 aggtgaaggc ggtcgatggc attgccgacg gcgccgccga tgagcagccc gatgctgatg
    23581 gccatcaggc gattcatttc accgcgcgcc agccaaatca tgagccccag cgccgcgacc
    23641 agcgcgaatg cggccagcgc cccctgcccg atgacgcctg cgccgagccc atagctgata
    23701 ccgaggttct tcacatagac gagatcgagg aaaggcagga caggcacccg ccccttctcc
    23761 tcgatcccaa aaatggcgag catccaccat ttgtgggcct gatcgacgac cagggtgacg
    23821 agggcaaccg acaaccccag tcgtgacagc ggtccccaca gccatcccgg cttcgccacg
    23881 gaaggcatgc cggtcactcg gctgccgcgg cggcgatgtt gcgcttctcg aagtcgcgca
    23941 cggcggctgc gtcgcgggcc gacagctccg gatattcggg gtccgagccc acatctggca
    24001 cgatgcgcca cgagcgcgca cacttgcggc cctccgcccg ccgcggcacc acgccgacac
    24061 ccggcacctc gggcagcgtg aaggcgctga ccgggggttc gctgggcacg acctgagccg
    24121 cgctcgtgat ggacacctcg gccatgtcca cgtcctcgag ggcgtccagc aggtcctcgt
    24181 cggcgacata gatgtcgggc gccgcctcga ggctcgagcc gatgcgcttc gcagcacgct
    24241 cgatttccag cgcacccgtg acaacccgcc gtacgcgccg gatctgctcc catttcttcg
    24301 ccagggcatc gtctcgccaa ggcttcggga tcttggggaa gacctgcaga tgcaccgaac
    24361 cgtcctcgga gggatagcgt tcgagccaag cctcctcggc cgtgaaggcg aggatcggcg
    24421 ccagccagat cgtcaagcaa ttgaaggtct cgttcagcac cgtcaggcag gcccgccgct
    24481 tcttgctggc gtagggctcg cagtagagcg catccttgcg gatatcgaaa tagaacgccg
    24541 acagatcgtt gatgcagaag ttcgacagcg cgtgcgacac acgcttgaag tcgtagttct
    24601 cataactctg tcgtacgagc gcatcgagct cggcgagccg gtgcaggatg tagcggtcga
    24661 gccccggcat ctcccgcgga cgcaccttca gcttctcttc gtaatgggcg agattgccga
    24721 gcagccagcg caacgtattg cggatcttgc gataagcctc gatgttggac ttcaggatct
    24781 ccggcccgat gcgcaggtcc tcagagtaat ccgcgctcgc cacccacatg cgcaggatct
    24841 cggcgcccga ctggcggatg acatcctgcg gcgccaccac gttgccgagc gacttcgaca
    24901 tcttgcgccc ttcctcgtcg acgacgaagc cgtgcgtcag gacaccctcg taaggcgccc
    24961 ggccgcgcgt gccacagctc tcgagcaagg atgagtggaa ccagccgcgg tgttggtcgg
    25021 aaccctcgag gtaaagcgag gccggccacg cgagatcctt gcgctcctcg agcacgaatg
    25081 cgtgcgtcga tccggagtcg aaccagacat cgaggatgtc gcgaacctgt tcccactcgt
    25141 ttatgtcgtc gacgagcccg gcgaggaacc gctccttggc accgtcgacg aaccaggcgt
    25201 cggcgccctc ggccttgaag gcctcgacaa tgcgcttggt cagctcgcgt gagcgcttcc
    25261 actcccggtt cgggatgtac tgctgcgtcg tcttgttgac gaaaacggta atcggcacac
    25321 cccaggcgcg ctggcgggaa atgacccagt ccggccgctg ctcgatcatg ccgcgcagcc
    25381 ggttctgtcc ggctgccggt acgaatcgcg tctcgttgat ggccttgaga gcgtactcgc
    25441 ggatcgtggc ctcgcccttc ttcccgggaa tcgtgatcgg cttgtcgatg gcgatgaacc
    25501 actgcggcgt gttgcgaaag atcaccggcg ccttggagcg ccacgagtgc ggataggagt
    25561 gcttgagccg cccgcgcgcg atcagtgccc cggccttgat cagctcctgg atcacggcct
    25621 cgttcgcgtc gccgaacttg cccttgtcgt cgatgacgcg cttcggatgc tcaccgccga
    25681 acaggggcac gtggcggaag taatagccgt cctcgccgac cgtgtgcggc acttcgggaa
    25741 tgccagcctc ggcgaaggag gcccggtgct cctcgtagac ggcgtagtcc tccgcgccgt
    25801 ggcccggcgc cgtgtgaacg aagccggtgc cggcatcgtc ggtgacgtgg tcgccgtggt
    25861 agagccgcac ctggaactcg tagccttggc cgtggaacgg gtgatggcag accatcgctt
    25921 cgagcgtcgc ctccgagacg tcgctcagcc gccgccagcc gttgattcgc gccgcaaggc
    25981 gcacgctgtc ggcgagcttg tcggccagga tcaggcgctc accgggtttc gcccaattgt
    26041 cggcgggcgc gtcggtgatc tcgtaaagac cgtactggat attgcggtta taggagatcg
    26101 cccggttgcc ggggatcgtc cagggcgtcg tggtccagat gacgacggcc gactggctga
    26161 cgttgtcgat ctcggccaat cctttgctga gctgccgcct ggtcactttc acctcggcga
    26221 ccgggaagcg gacccagatg gttgggctag tgacctcgtg gtactcgacc tcggcctcag
    26281 ccagcgccgt gcgctcgacg accgaccaca tcaccggctt cgagccgcga tagagcgagc
    26341 cgttcatggc aaacttgatg agttcgcccg cgatcttggc ttccgcatcg aagctcatgg
    26401 tagcgtaggg atggtcccag tcgccctcga cgccgagccg cttgaactcc tcgcgctgga
    26461 cattgatcca cttctcggcg aagacgcggc agtcgcgcct gagctcgatg atcggcacag
    26521 agtccttggt cttgcctttg gcccggtact cctcctcgat cttccattcg atcggcaggc
    26581 catggcagtc ccagccaggc acgtagttgg cgtcgcgccc catcatctgc tgggagcgga
    26641 cgacaatgtc cttcaggatc ttgttgaggc cggtgccgat gtggatatga ccgttcgcat
    26701 acggcgggcc gtcatgcagg atgaatttct tgcggccctt tgcggcttcc cgcagccgac
    26761 gatacatgcc gagctcgctc caccgctgga tgagccgcgg ctcgagttcc ggcaggccag
    26821 ccttcatcgg aaacgccgtg cgtggcagaa agagtgtctt gctccagtcg cggccctggg
    26881 ggcccggctt tccgttgtcc ttgggcatga gcggtcatcc ggatttcggg gtcaaccggc
    26941 cttctagcaa cctcagctcg cgaaacacaa gtggaacctc gggcgcaggg atgtttcaac
    27001 cctccgcaag cgggaagccg gcgagcggcg attgcctgtc cacagctgcg atgatctcgc
    27061 gtgcgcgagc acagtccgcc tgcatctgcg ctatgagcgc ctgcgcatcg gggaagcgct
    27121 ggtccccacg aatgaagtcg aggaactcga cctcgatctc cttgccgtag aggtcgtcct
    27181 cgaaatcgaa caggaacacc tcgagcaccg gcgcgccatg atcgaacgtc ggccgcgtgc
    27241 cgaggtaggc cgcgccgctg taacgctcgc ctgcgatgta aacccgtacg gcgtagatgc
    27301 catgggccag cgccgtccct ttcggcagga caatgttggc cgtcggaaag cccaatcccg
    27361 tgcctcgctt cgcgccgccg gtcacgcggc cggcgacacg ccaccagtgg ccgagatcga
    27421 gggctgcccc gcgcacgtcg ccagcggaaa gcttcgcccg gataccgctc gacgagaaca
    27481 cctcgcccgc ttcggcgacc ggcggaatga ccgtgacccc gaacccgtgc tcgcgcccgg
    27541 ccgcgcgcat cacctccgga ttaccgccgc gccccttgcc aaagaagaag tcgtagccga
    27601 tcacgacgtg actcacctgc aaggcgccga cgagcacctc gtcgatgaag cgggccgcgg
    27661 gcagcccggc gaggctcgca tcgaaggtga gcacgatcac gaagtcgagc ccgagacgct
    27721 cgatcagcct caccttttcc tgcagggtgg tcagcgtgaa gtgcggctca tcggggcgga
    27781 agaactcccg cgggtgcggt tcgaacacca tcaccccggc cggcttgccg gtcgcccgcg
    27841 cctgcttcag cgcggtcagc agcagggtct gatggccgcg gtgcacgcca tcgaaattgc
    27901 cgatggcgag cgcggcgccc tttatttcgg gcggaacctg ccggtatccg tggacgatgc
    27961 gcatgggttt gcacggccgt gggcgcgctt cgcggtttca ccttatcaag gcgcatggaa
    28021 tagccgcctt gagagaggtt gagaagggcc agtctgtacg gggggcgtaa cggatctgga
    28081 gtggcgagat caggcggggc gggtcggaac catcagcatg gcctcgccct cgagaacggt
    28141 cttcccgtcg accgagcaga cacattcgaa acgcgcgcga tgcttggccg ggaacagctc
    28201 gacgaccttg accttcgcgg tgaccgtctg cccgatcttg accggagcgc ggaaattgag
    28261 cgtctgcgaa atgtagatga cgcccgggcc gggcatcttc gtcccaaacg ccgccgagat
    28321 gtagctcgcc gtcagcatgc cgtgggcgat gcgctccccg aacatcgtct tggcggcgta
    28381 gtcggcatcg aggtggacgg ggttgcggtc gcctgtcacc tcggcaaagc gattgatatc
    28441 agcctctgtc acgagtctct ggaagctggc ttccattccg agctcaagat cttcgaaata
    28501 ataggttttc cgcgtcaaca cggtcatgat tttctcctcg cgcccccgca agaataaggg
    28561 gcgtcgcccc cgggttcccc cagcccgagg acagtaatca gcctatgttt gcactgcaat
    28621 aacgaatgag ccggagactg ttccgctgtg aaacagtgtc gccgcatcgc ggcgccgcag
    28681 ccctcgcaag ctgccctcag gcgttgcgtc cggaggaagc gccctatata agcaagggat
    28741 tctccccctc atcttccgaa tagtcaggtc ttcgcgggcg gccgtggagg caggaggaac
    28801 agactatatg agcgaccgca gacccttgat gccgaaatcc acggcagtgt ggctggttga
    28861 gaatacttcg ctcacattcg aacagatcgc cgagttctgt ggcatgcacc tgctcgaggt
    28921 caaaggcatt gccgacggcg atgttgcggc cgggatcaaa ggcatggatc cgattacgac
    28981 cggccagctc acccgtgagg agatcaagcg cggtgagcag gacccgaact accggctgaa
    29041 gctggccgag cccaagatcg agattcccca ggtcaagacc aagcgcgggc cgcgctacac
    29101 gccggtctcc cgccgccatg accggccgaa cgccgtactg tggctgctgc gcaatcatcc
    29161 cgagctgaaa gacagccaga tcatgcggct cgtgggcacc acgaagccga cgatccaaca
    29221 gatccgggat cgcacgcact ggaactcggc gaacctcgtt ccgcaggacc cggtgacgct
    29281 cgggttgtgc tctcaaaccg acctcgatgc agaggtcgag aaagcggccc ggcgcctcga
    29341 gaaggagcgc aagatggagc cggccgagac cgggggcacg cttttgcccg tgtccgagac
    29401 ggtcaagccc gccgagatcc ttcacgagga gcccgaagcg ccccacgagg agactcccga
    29461 ggaggaggag gcgcgtatgc tcgccagatt gcgcgaaatg ggtagcggct ctgcccagaa
    29521 cggcgacgag gataatgaag aagttcccga aggcgaaagg gccgaggaac cgaacacctg
    29581 acgacgccgg agaccaccgg cgcgccgggc agaaccagtt cgatgcgccg gcgctgccgt
    29641 tagactctcc ccgtgccaaa acatgcgcgg cgtggctatc gggccgcggc aagccacgct
    29701 caccctgctg ggcgaggcct ttcactcatc atgttgggga ttgcgatcgg cgctttacga
    29761 caaccccgcg ccgctactct tcagccgctc gatgatggtt tgactgatgc ccggcacacg
    29821 ggcgacatcg tcccagctcc gaaacggccg cctgtagacc agcgcctgga tccgtgccgg
    29881 accaactgcc gagacagccg ccagcacatc ctccggagcg gtattcagat cgatccggca
    29941 agaccggtcg ccttgagcag ccgcctcagg atgatcatag cgtgtcggag agaccatggc
    30001 ttggtgttgc ccctacttag ccaagacgct cgcagaacga cgggacgggg gaaaagttcc
    30061 tagggcggcg agctctcccg cgccgatcgg gagcccaggc gatcacatat tgtttcagtg
    30121 atagagatcc gctcgcgtcg gcgggagctt gggtggccgc ttggcggact tgctggccaa
    30181 agtctggtag atgcgggcgg ttccacggga aaaggcaagg gacacaccgg caaggtaggc
    30241 gacccagatc cggtacttct cctccccgac gaaggcgatg gcgcgttctc gattggccgt
    30301 cagccgttcg caccaaagct ggcaggtgcg ggcgtagtgc agccgccagg cctcgacgtc
    30361 gtgaacctcg aagcccgcgc gctccatggc gacgatcgaa tggccgatgt cgtcgagctc
    30421 gccgccagga aagatgtact tggcgattgc gcgctgctcc ggccgcatcc ggcgcccgcg
    30481 ccaggagggc ctcggcgccc ggcgcgagat ggcgtggttg aggaatagtc cgtccggcgc
    30541 gagcagagat tgcacttttg ccatgtaacg cgggatgttg gcgaggccga tgtgctcgta
    30601 catgccaact gatgcgatct tgtcgaatgt tcccctaacg gagctgtaat cctggagatc
    30661 gagggtcact tggccctcaa gtccaagccg cttcaccttc tcgcgcgcat agccaagctg
    30721 cttctccgac agcgtgatcc cgtgcgcccg gacgccataa tggcgcgcgg catggcaaac
    30781 gaggccgccc caaccgcagc cgatgtcaag caggcgctca cctggtttca gccgtagctt
    30841 gcgacagatc atgtccagct tgtcgcgttg cgccgtggcg agatcgttcc cccagtccgt
    30901 gaaataggcg cacgagtaga ccatctccgg atcgaggaag agcgcgtaga attcgttcga
    30961 cagatcatag tgaaactgca cgaacgcctt gttgtcgcgt cggcgttgcg accgaccggt
    31021 gatgtcaccg ctgaaaccgt gcacatccgc cgttctgtct gcaggcacga gcaggaacgg
    31081 cgccagtgtc ttcaggagaa cgagcttgtt cattcccttg aggcttgccg accggccgtt
    31141 gcgattgagc tgtcggccga agtcgatcag cgttccgccc tcgaaatcga tgcgcccttc
    31201 agcgtagtgg cggatcagat tgtcgatcgt tggccggcgc agcagcgagc cgatcacccc
    31261 cggcccggca atggagatcg tgaacgggcc gccaaccgcc tcccccagcg gaaccagcga
    31321 gccatcccaa agccgaacgg aagcatcgat gtcgagccgc tccgcaatga gctctacgag
    31381 cttccgcgcg gctgaaagga gacgcgcgtc ccttgctgat ggcatctgcg tccatcccac
    31441 attatagatc ggaaagtccc ggcgtcggca cgctcacacg gattcctgcg cagacgcaat
    31501 ccccttcgcc gatttggtgt tgaacggcat gattttacct agccggattt gaatccggca
    31561 gctgtcaaaa tctggtcgag cattccccag aaaaaatccg cgccgggata gcccacaatc
    31621 cgccccaatt ccacgccgtc gcgcacgacg atgaaagttg gcgaataaat gactttcgca
    31681 aactttttca catccggatg attgcgcggc cgtctgacga gcggggcaaa atgtccttcg
    31741 gcgctggcgg cataagcgac ccccacctcc ttattccagc gagcgcaata aggacagccc
    31801 cggtcttcga tcataatgag ctgcacgccg cttgccgtgc cgggtggggc cgtgctggca
    31861 gccatggcgg acggcgtgca tggccgtccg ctcaaagcga gcaacatcat ccacaggcaa
    31921 aacttggtgc agtcacggcg gctcagaaga aagccgttat ctcgagaatc ggtcgccatg
    31981 ggagatcatt ctcagatttg ccggaagaca ttctggtacg ctaatgtctg tcgacgcaag
    32041 cccttcaccg gcactgaccg ggaaagactt caacacgatt ttgcgagacg cttcgccaag
    32101 ggccttacgc tcaaagccac tgcgaagaga cgtgtgacag cggttcagga aggattgacg
    32161 acatggcggc gcccctggag acagatgtga agaacagcct tctgcgcgcg gcgatcagcc
    32221 ggcgtggcct cctggtcggc gtggcaggcg ccacgactgc cttgctgctg tcggacagtg
    32281 acgccctggc cgctgccgac gacggctggg aagcggcgct caagaaaatc atcggcgagg
    32341 cgacgccggt caacgataaa ataaagctcg aaatgccgga aattgcggaa aatggaaaca
    32401 cggttccctt ctcggtctcc gtcgacagcc cgatgaccac cgaggattac gtgaaatcag
    32461 ttcacgtaat ttcgacgggc aaccctcagc cggtcatcgc aacatttcat ttcaccccag
    32521 agtccggcaa ggctgccgta gccagccgca tgcggcttgc gaaaactcag gacatcatca
    32581 gcgtggccga gctgagcgac ggcaagttcg cgatgacgaa aaagaccgtg aaggtcacca
    32641 tcggcggttg cggcggctag ccgtcagcgg gcgcctggga aggcaatcaa ttcggcgagg
    32701 aaatacacga catggcgacg aagccgcgca tcagggttcc cgagaccgtc aaggccggcg
    32761 aggtgatcga ggtcaagacg ctcatctctc acatcatgga aaccggccag cgtaaggatc
    32821 aggacggcaa gccgatcccg cgcaacatca tcaacacgtt cacggccacc ttcaacggca
    32881 aggaagtgtt caaggtggac ctgcatcccg gcatctcggc gaatccatac atggccttcc
    32941 acgt
//
LOCUS       DASVDU010000108        24339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_223429, whole genome shotgun sequence.
ACCESSION   DASVDU010000108 DASVDU010000000
VERSION     DASVDU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 24339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24339
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223429"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..871)
                     /locus_tag="VF226_06850"
     CDS             complement(155..871)
                     /locus_tag="VF226_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7073744.1"
                     /translation="MSVENAAPRRDCLPDDAPHVLIVDDDQRIRDLLTRYLIEHGYRV
                     TPASDASAARAAMRGLKFDLVILDVMMPGESGLDFARHLKTTSEVPICMLTARSEPED
                     RIEGLEAGVDDYLPKPFEPRELVLRVKNILRRGQANHANLDEVRMGEFVFYIARGELK
                     RGDETIKLTERERDLLRQFAQRPGVAIARHELAGDDSTGSERAIDVQINRLRRKIEVD
                     PSNPVYLQTVRSKGYILYTD"
     gene            complement(895..1446)
                     /locus_tag="VF226_06855"
     CDS             complement(895..1446)
                     /locus_tag="VF226_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7073745.1"
                     /translation="MTDIYAAAGARPVKSGSQDVTLEPAEEVGREQLIALVELLFFAY
                     RDFTGEADAILAEFGFGRAHHRVLHFVNRHPGLRVADLLDILKITKQSLARVLKQLVD
                     RGYVLQEAGPQDRRERLLYLTNKGRDLANRLLELQNARIAAALAEAGDGAASVSSRFL
                     AGMITGGERSRIEALIFGPGRRA"
     gene            1629..2522
                     /locus_tag="VF226_06860"
     CDS             1629..2522
                     /locus_tag="VF226_06860"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX7073746.1"
                     /translation="MASLVPYHDRDGFIWYDGKLVPWREAKLHVLSHGLHYASAVFEG
                     ERAYGGEIFKLEEHTQRLIDGARTMDFEIPYTAEEINAACRETVKANNLVDCYVRPIA
                     WRGSEQMGVSAQQTKIHLAIAAWDWGSYFDPSERMRGIRLTHAKYCRPDPRTAPAKTK
                     AAGLYMICTLEKHRAERAGYADALMLDWRGHVAECTGANIFFIKDGVLHTPTPDCFLD
                     GITRRTVIGLAKKRGIEVQERTILPEELGSFSECFLVGTAAEVTPVREIGEHNYQPGR
                     ITESLLNDYMAAVQPQKSAAE"
     gene            2876..3535
                     /locus_tag="VF226_06865"
     CDS             2876..3535
                     /locus_tag="VF226_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073747.1"
                     /translation="MADNGQTAVAWEMLSSVVAMRAKARENKLPKLLSSLHGVEVELA
                     RIIAGTEAPQDATINGSVRNQPAAIAAAAGYIDETYSDDTGLEPEPPPAPRSRSGRRG
                     GGPRGDQQLRAYAKKYLDITRQLKELVERGEAAATGNHFGDAMDEDWEAESDALLSDQ
                     HDLSFTIAGLKATTFEGLVDKAAVLDDLIEENTDDPVQVLARSLVRDVIAMGQNGKSG
                     R"
     gene            complement(3911..4066)
                     /locus_tag="VF226_06870"
     CDS             complement(3911..4066)
                     /locus_tag="VF226_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073748.1"
                     /translation="MQLSHAQRLEHYHLTLALGDRATGTYIDWMLKMPHACYSEGRIS
                     RGALRNS"
     gene            4285..7800
                     /gene="dnaE"
                     /locus_tag="VF226_06875"
     CDS             4285..7800
                     /gene="dnaE"
                     /locus_tag="VF226_06875"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7073749.1"
                     /translation="MAGVGFVHLKVHSAYSLLEGALPIGKLAKLCQELGFPAVALTDT
                     NNLFGVLEFSDKLSSSGIQPIAGVSLAIDFEEKKPAHMGGPASSSDALARRDGSIALY
                     AMSERGYANLMKLVSRAHLQVGENEGPHITISQLRGAAEDLIVLTGGPDGPIDTALRD
                     GQKTLAEARLEALARICGDRLYVEIQRHGLEKEALVEPDLLDLAYNLNLPLVATNEAY
                     FASPDDYEAHDALLCIAEGRYLVEDDRRRVSPEHYFKSAEEMMALFQDLPEALDNTIE
                     IAKRCAFRPNGRKPILPRFVATSERASDEDQLAAEAAELKRQAEQGLKARLAANPLAP
                     GFTEEDYWKRLAFEVDIITRMKFPGYFLIVADFIKWAKAQGIPVGPGRGSGAGSLVAW
                     SLTITDLDPLRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIRYVQRKYGADRVAQI
                     ITHGKLQARAVLRDVGRVLQMPYGQVDKLCKLVPNNPANPVSLAEAIAGEPRLQEARD
                     SDPLVARLLEIAQKLEGLYRHASTHAAGMVIGDRPLDELVPLYRDPKSNFPITQFNWK
                     QVEKAGLVKFDFLGLKTLTVLKKAVDLIERCRGVKIDLASLPLDDRPTYEMLARADTV
                     GVFQLESTGMRESLKRLKPDRFEDIIAMVALYRPGPMDNIPTYINRKHGEEPVDCLHP
                     ILEPILKETYGVIIYQEQVMQIAQVMAGYSLGEADLLRRAMGKKDKAEMAKQQARFVE
                     GAVKNGVAKKDAVYIFELVDKFAGYGFNKSHAAAYALVSYHTAYLKANYREEFLAASM
                     TLDMGNTDKLSVFAAEARRSGIALKPPCVNASAVDFLPEPARREGEAGAIRYSLAALK
                     NIGSGAVESIVSTRQKDGPFKDLSDFASRLDTKALNKRALETLAAAGAFDALGVDRAL
                     VHGNVDQIMALAHRIASDAASGISDLFGGGSSSAPQLDLRPVKPWTPMELLEKEFEAI
                     GFFLSGHPLDQYESVLSQLGVQTWSEFEAATERGVSAGRLAGVVVSARERKSQKGNKF
                     AFAMFSDSTGQFEAVIFSDTLAQSRDLLEPGTPVLLTVEAEREGDTVKMRVQSLESLD
                     KAAGAVQRGLKLVIGGSAVLARRAEIAGIRDLLKPGRGEILILLKLDEKGREVELAVP
                     GRYDVSPMVKGTLSTVPGVLEVREI"
     gene            complement(7848..8678)
                     /locus_tag="VF226_06880"
     CDS             complement(7848..8678)
                     /locus_tag="VF226_06880"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7073750.1"
                     /translation="MTDGISTFTEALAAWLGGVLPNVAVALLMLAVGWAVAGWAERGT
                     GRLLDRARMDMTLRSVTTALVRYAVLIVVVVAALGQVGIQTTSVLAALGAIGLAIGLA
                     LQGTLANIAAGIMLLWLRPFRVGDYIEAGNIAGTVKEVGLFASELHSWDGIYQFVPNS
                     ELWSKRIINYSRLEQRLVDLKFSISYSDDIDRAREVLLSLARADDRVRADPAPDCFVD
                     ELADNAVILALRVWTATATYWPVRRSLTERGKIELEAAGLSRPFAQRVVHMKQVANGE
                     "
     gene            complement(8794..9957)
                     /locus_tag="VF226_06885"
     CDS             complement(8794..9957)
                     /locus_tag="VF226_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7073751.1"
                     /translation="MAADDKAHQRSVFGAEGASSPKRRPLALGLLVLAAMALRRRLGE
                     PKPAHAGNKAVAKVTRPGEPAGSQAQAGSSGGRGHEAETPSEIPAHGWKDIAVRVYRN
                     IQDHRLMLIAAGVTYYSLLALFPALAALVSLFGLVADPEAIQQQLANMEGVVPEGALQ
                     IIGDQISQLQAQGGGTLGLYFFIGLAISLWSANAGVKSIFEALNVVYDEPERRSFIQY
                     NLQALLFTLGGILFIILAVTGIVVLPAVLAFVGLQSMAGWIISIVRWPLLLIAILLGL
                     ALLYRYGPSRENPEWRWVSPGSIFTAVVWLAGSILFSWYVSNFGSYNKTYGSLGAVVG
                     FMVWMWLSATIVLIGAEINAETEAQTRKDTTSGAPRPMGQRGARAADHVASGA"
     gene            10185..11168
                     /locus_tag="VF226_06890"
     CDS             10185..11168
                     /locus_tag="VF226_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7073752.1"
                     /translation="MLPTFTMRQLLEAGVHFGHQAHRWNPKMAPYIYGVRNKIHIIDL
                     TQTVPLLHQALVKISDTVAEGGRVLFVGTKRQAADAIAEAAKRSAQYYINHRWLGGTL
                     TNWKTISQSIRRLRQLEDILADPQGRTKKEVLNLTRERDKLNQALGGIKEMGGTPDLL
                     FVLDTNKEQIAIQEAKKLGIPIVAVVDTNCDPDGIDYPIPGNDDAGRAITLYCDLAAR
                     AAIDGIQRAQAAAGVDIGAAEAPPEEQLPAEFKGIEAPRGEPDDLKRISGITPKLEQR
                     LNDLGVFHFWQIADLDADAVRSLDRQLKLKGQIEQEDWVGQAKRLVEAAAA"
     gene            11289..12227
                     /gene="tsf"
                     /locus_tag="VF226_06895"
     CDS             11289..12227
                     /gene="tsf"
                     /locus_tag="VF226_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7073753.1"
                     /translation="MTQISAAMVKELRDKTGAGMMDCKQALIENAGDIEASVDWLRKK
                     GLSKAAKKSGRVAAEGLIGVAVEGKTGTLVEVNSETDFVARNAQFQEMVREISKLALQ
                     AGGDLSKLLALPFPGKTVSVEEHVREMIATIGENMNVRRTAQLTAGADGIIVDYIHNR
                     VAEGLGKMGVLVALESTGDKAKLNEFGRQIAMHIAAAAPLAITVEDLDPAVIERERKI
                     YEEQAAASGKPADIVAKMAEGRLRKEFFQQVVLMQQIFVIDGKSTVEQAVKEAAGTVG
                     APVKITGFVRYALGEGIEKKEEDFAAEVAAAVSGTR"
     gene            complement(12300..12680)
                     /locus_tag="VF226_06900"
     CDS             complement(12300..12680)
                     /locus_tag="VF226_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX7073754.1"
                     /translation="MSDGQHDHVIRWAVVLAGLIGAAGIASAAAAAHSGAEVRLLGAA
                     SAICLANAPALLVLGLSGRRLRLGALCAALLALGTILFAGDVGLRAFLDRGLFPMAAP
                     TGGSTMIIGWLLVGMSALFTRRGK"
     gene            complement(12717..14225)
                     /locus_tag="VF226_06905"
     CDS             complement(12717..14225)
                     /locus_tag="VF226_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7073755.1"
                     /translation="MAAYAVGRAAKSFPDKPALIVIADPLQPPAEVWTFAKIESAVLR
                     IAAGLKAKGLEPGARIMIRLENTSTYALLFFGAIAGGYVPLPTASQLTEREAAFLLQD
                     SGAAAIALADGLPVSDIPAGVLVLDGEEVRALADHPIAGTYADSSADDPAYLIYTSGT
                     TSKPKGVLHAHRAAWGRRPMYQGWYGITSADRVLHAGAFNWTYTLGTGLTDPWANGAT
                     AIVYTGPKDPAVWPRLIRSAEATIFAAVPTLYRQILRYTDVAPGSLGALRHGLIAGET
                     PPPGLFENWTARTGLELYEALGMSELSTYISSSPSVPRKPGAVGKPQPGRSVVILPAE
                     GGTEPLPAGEQGLLAVHRTDPGLMLGYWNRPDEEAEVLRGDWFIGGDLARMDGAGYVT
                     HCGRANDVMKALGYRVAPQEVEAVLAEHPSIAEVACAEVQVRADVSVIGAFVVLREAA
                     CRGDSIAQEIQQFAAERLAPYKCPREIIFLDALPRTANGKLRRGELKSWRKA"
     gene            complement(14282..16087)
                     /locus_tag="VF226_06910"
     CDS             complement(14282..16087)
                     /locus_tag="VF226_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7073756.1"
                     /translation="MKLLLALASVLMAALLPATLVAGTSVGQHGIAMHGTPALPPDFA
                     HLPYVNPDAPKGGRLRMGALGTFDSLNPFIIKGVTPSGLREYVYESLMARSQDEPFTL
                     YGLIAERVEVPDDRSSITFYLRPEAHFSDGRPITPADVIHSHALLKEKGWPYHRSYYS
                     KVARAEQIGPHAVRFTFKEGGDREIPLILGLMPILPAHVMTAEAFDRTSLEPPVGSGP
                     YVVDRVEAGRSLVFRRDPNYWGKDLPINRGRYNFDEIRIDFYRDAASLFEAFKAGQID
                     VRAEEDPARWAEGYGFQAVADGRVLKSEFKTGLPSGMSGLVFNTRRPVFADQRVRRAL
                     ILMFDAEWINRSLFHGLYVRTTSYFDRSDLASTGHPASEAERKLLEPYLEEVDPDVLE
                     GRSHLPVGDGSGNNRAALREAYKLLTEAGYKLDGSRLVNAKTGEQLSFEFLASNRAQE
                     RIMLSFQTNLERLGINVDIRQVDAAQYWARLKSFDFDMIQWTWGASLSPGNEQINRWS
                     SRAADIEGSLNYPGVKSPAADAMIEALLAARTYDELTTAVRAFDRVLLSGDYVIPLFH
                     VPKQWIAHWSHLHAPKETALFGTSFDTWWQAGEAP"
     gene            16406..17203
                     /locus_tag="VF226_06915"
     CDS             16406..17203
                     /locus_tag="VF226_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX7073757.1"
                     /translation="MANIGWFGSGVARAAAGVAVAFGLQMAISGAALAQQGKSQPKQA
                     QQSQPKQAKQEQKQSAWVKLCEKAQIAHMGKDGKPVREEKNICLTHHERLDGNTGMVL
                     VSAAIRDVEGMDKEHLMIMVPLGMAIPPGIRAAVYTPEQWEKAQKNEKIDESELKPIS
                     LHYSLCHPAGCTAEVEASKELIESMKKGGGLMVLAINANGQPIAFPVPLDGFTAAHSG
                     PPVDNKAYAEARSQLMQQIRQRQAELVKQAQEKQKAAQKEKAAEKKK"
     gene            complement(17440..17772)
                     /gene="hspQ"
                     /locus_tag="VF226_06920"
     CDS             complement(17440..17772)
                     /gene="hspQ"
                     /locus_tag="VF226_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX7073758.1"
                     /translation="MSKISVAKYAIGQVVRHRFYPFRGVIFDVDPVFDNTEEWWLSIP
                     EEIRPRKDQPYYHLLAENAETEYVAYVSEQNLMLDDSGAPLRHPKILELFSEAENGRY
                     EATFVQPH"
     gene            complement(17933..18982)
                     /locus_tag="VF226_06925"
     CDS             complement(17933..18982)
                     /locus_tag="VF226_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7073759.1"
                     /translation="MPSTRTPARFYSPLAIGAPEPLRQLPVRLERMIHFVPPHNAKIR
                     ARLAETIASVDVLLGNLEDAIPAEDKEAARRGFIEMAQQHDFGTTGLWVRINALNSPW
                     ALDDIIEIVSAVGSRLDVMMLPKVEGPWDIHYLDQLLAQLEAKHQIDRPILIHAILET
                     AEGVSNVEEIAAASPRMHGMSLGPADLAASRGMKTTRVGGGHPDYGVLADAGSGGPRG
                     FFQQDLWHYTVARMVDACVRNGIKPFYGPFGDFSDDAACESQFRNAFIMGCVGAWSLH
                     PKQIAIAKRVFSPDPNEVAFAKRILEAMPDGTGAVMIDGKMQDDATWKQAKVIVDLAR
                     LVAAKDPGLASAYGI"
     gene            complement(19058..19969)
                     /locus_tag="VF226_06930"
     CDS             complement(19058..19969)
                     /locus_tag="VF226_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX7073760.1"
                     /translation="MPAALRDLQFRAEYVVLRTVVGLVRALPLSLAIRASAKAWRFLA
                     PIVSPVRHQRALENVAIAFPEKSEAERHDIVMGHWENLGRVMAETMQIDRILRDPSRI
                     EIENFALFTRYKDKLGPAIGVSLHMGNWEFAIWPLVVAGANPAAIYRSVNNPYVDRYL
                     RAQRKDLYPGGLFGRGRVEGDHGESQRTARIITDYVRRGGRLGLVCDLFDRTGIPVPF
                     FGKPARTQAIAAIIARRVGARLWLARCARIGTESRFRVQIRELKVPRTANQADDIRTI
                     MTEMQLQFEEWIREAPEQWMWSNRRWS"
     gene            20185..20607
                     /gene="arfB"
                     /locus_tag="VF226_06935"
     CDS             20185..20607
                     /gene="arfB"
                     /locus_tag="VF226_06935"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX7073761.1"
                     /translation="MLRVTDSISIPEVEIEEHFVRASGPGGQNVNKLSTAVELRFDVA
                     ASESLPPPVRDRLRKIAGRRMTKEGVLVIRAERHRTQELNRADARERLIALVRRATMQ
                     PKRRIPTRPTKASRERRLESKVRRGEIKRQRSAKPTLD"
     gene            20618..21853
                     /locus_tag="VF226_06940"
     CDS             20618..21853
                     /locus_tag="VF226_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7073762.1"
                     /translation="MTARRLSATCCIAGGGPAGIMLGFLLARAGIPVVVLEKHADFLR
                     DFRGDTIHPSTLELMAELGLDDAFLKLPHEKLVRVSGQFGDRPLVFADFSRLPVRHPY
                     TAIMPQWDFLNFLAEQGRRYPGFTLLMETTAEDFLYERDRIAGVKANAPDGPLEIVAD
                     LVVAADGRHSVLRSRSGLPLVDLGAPMDVLWFRLSRRPDDSRETIGRFAPGRLLVRIN
                     RGDYWQCALVIPKGGADDLRQRGLDAFRRQVGALLPWAESRITLDVRSWDDVKLLDVR
                     VDRLRQWYRSGLLLIGDAAHAMSPIGGVGINLAIQDAVAAANILWQPLRDKQLETHHL
                     AAVQQRREWPTRATQQLQVIVQNRVIRPALSAEAPLRPPLLLRLAASIPMLRGLTARL
                     VGLGLRPEHIGSPELPPAL"
     gene            complement(21858..22727)
                     /gene="mazG"
                     /locus_tag="VF226_06945"
     CDS             complement(21858..22727)
                     /gene="mazG"
                     /locus_tag="VF226_06945"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX7073763.1"
                     /translation="MTSSLSPSRDIAVLLQIMRRLRTPVTGCPWDLKQSFETIAPYTI
                     EEAYEVADAISRGDLAHLREELGDLLLQVVYHAQMAEEQQAFDFGDVVEGITTKMIRR
                     HPHVFGDEAARSAAVPEDFWERAKAAERAEKARAGASSEGSAGSESILADVPLPLPAL
                     TRAVKLQDRAAAVGFDWPGLGPVMDKLKEELTELDDAIAAGAEDRISEEFGDLLFVIA
                     NVARHLKIDPEAALRAANDKFVRRFRYIEEALAARGKRPADSSLEEMDALWDEAKTSE
                     ADKPGAPETRKES"
     gene            complement(22867..23742)
                     /locus_tag="VF226_06950"
     CDS             complement(22867..23742)
                     /locus_tag="VF226_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073764.1"
                     /translation="MRNTVNFAALLMGTALAVTTAAQAQQSQQFNGQQMGQQFGTQQF
                     GQPMTGQMQGHQQLTQQSIQNFFQQAQNVLQQTARTQDPNALRQYINQYVAPDAEFTT
                     ISQLFVRDRHVATTVAEASDEMVSDALGYAANMLQGRKLVSNYQINIDVRDIDISPSG
                     DTARVKTVVHERGILTGPMAQHVASRVGQARQRMGQLRQQWQQGQTGQGQQQFGQLGQ
                     GQQPWTQTPQMQSGQPQGGMTSGMGSGTGAGGGTQAQGIPFETLANCTNDILLEQGQI
                     KLGNTFCRGTMHLGQ"
BASE COUNT         4492 a         7676 c         7939 g         4232 t
ORIGIN      
        1 tcatgcgcac gagccgggcc aacagggatc gtacccgtac ccaccaaggc tcggccatcc
       61 tctcgccggt atcgtcgcgc actgcgacca tgacgatctc gttccatgga ggcgccggca
      121 gttgtccacc cttcggcgcc gctttctctg cccgtcagtc ggtatacagg atgtagccct
      181 tgctgcgcac cgtctgcaga tagactgggt tggagggatc gacttcgatc ttgcggcgca
      241 ggcggttgat ctggacgtcg atggcacgtt cactgcccgt cgaatcgtcg ccggcaagct
      301 cgtgacgggc gatggccact cctgggcgct gggcgaactg gcgcagcagg tcgcgctcgc
      361 gctccgtcag cttgatggtc tcgtctccac gcttcagctc gccgcgcgcg atgtagaaga
      421 cgaactcgcc catgcgcact tcgtcgagat tcgcgtgatt ggcttgtccg cggcgcagga
      481 tgttcttcac gcggagcacc agctcacgcg gctcgaaggg tttcggcaga taatcgtcga
      541 cgcctgcttc cagcccctcg atgcgatcct cgggctccga gcgcgcggtc agcatgcaaa
      601 tcggaacctc tgacgtcgtc ttcaaatggc gcgcaaagtc gagcccgctc tcgccgggca
      661 tcatgacgtc cagaatgacg aggtcgaact tcaggccgcg catggcggcc cgagcggcgc
      721 ttgcatcgct tgccggcgtc acgcgatagc cgtgctcgat gaggtagcgt gtcagcaggt
      781 cacggattcg ttgatcgtca tcgacgatca gcacgtgcgg agcgtcgtca ggcaggcagt
      841 cacgacgcgg cgccgcgttt tcgacggaca tgcttcccct cccagttgcc ccagtcaggc
      901 ccggcgcccc ggcccgaaga tcagcgcctc gatccgggag cgctcaccac cggtgatcat
      961 gccggcaaga aagcggctcg acacggaagc ggccccgtct cccgcctctg ccagcgcagc
     1021 cgcgatacgt gcgttctgca actccaagag ccggttggca aggtcgcgcc ccttgttggt
     1081 caggtaaagc aggcgctcgc gccgatcctg cgggcccgcc tcttgcagaa catagccacg
     1141 atcgacgagc tgcttcagaa cacgcgccaa actctgcttg gtaatcttga ggatatcaag
     1201 cagatcggca acccgcagac ctgggtgacg gttgacgaag tggagaacac ggtggtgggc
     1261 gcggccgaag ccgaactcgg ccagaatggc gtccgcctcc ccggtgaagt cccggtacgc
     1321 gaagaacagg agctcgacga gagcgatgag ctgctcacgg ccgacctctt cggccggctc
     1381 gagcgttacg tcctggctcc ccgatttcac cggtcgggcc ccggctgctg catatatgtc
     1441 agtcatgttg acttatttgg atgccattgt tacccgtggc aagtgccttc gtgcactgga
     1501 ctgcgggcct gaccgctggc aacctcgccg gcttcgggat ggtgagggag ggcttctcat
     1561 ccgcagcagg atcctgacaa gcagcgtcac tgaggccgat cacaatgcca tctccaagga
     1621 gatagaaaat ggcttcgctc gttccttacc acgaccgcga tggattcata tggtacgacg
     1681 gcaaactcgt gccgtggcgc gaggcaaagc tgcacgtact cagtcatggc ctgcattacg
     1741 ccagcgcggt attcgagggg gagcgcgcct acggtggaga gatcttcaag cttgaagagc
     1801 acacgcagcg cctgatcgac ggtgcgcgga cgatggattt cgagattccg tatacggccg
     1861 aggagatcaa cgctgcgtgc cgggagacgg tgaaggccaa caacctggtc gactgctacg
     1921 tgcgcccgat cgcctggcgc ggcagcgagc agatgggggt ctccgcgcag cagacgaaga
     1981 tccatctcgc cattgcggcc tgggactggg gctcctactt cgatccgagc gagcggatgc
     2041 gcggcattcg cctgacgcac gcgaagtatt gccggcccga tccgaggacg gcgccggcca
     2101 agacgaaggc ggccggtctc tacatgatct gcacactcga gaagcaccgg gccgagcggg
     2161 cgggttatgc cgatgcattg atgctcgact ggcgcggcca cgtggccgag tgcaccggcg
     2221 caaacatctt cttcatcaag gacggcgtgc tgcacacgcc gacaccggac tgtttcctcg
     2281 acgggatcac gcggcgcacg gtgatcgggc tcgcaaagaa gcgcggcatc gaggtgcagg
     2341 agcgtaccat cctgccggaa gagctcggct cgttctccga atgcttcctg gtcggcaccg
     2401 cagccgaggt gacgccggtg cgcgagatcg gagagcacaa ctaccagccc ggccgcatca
     2461 ccgaatcact gctcaacgac tacatggcgg cggtacaacc gcagaaatca gctgccgaat
     2521 aggaatctgg cagttcacaa ccttgttacc gggcgggtcg gaacctcgtg ttccggcccg
     2581 caaatttctt tggagtttcc gcttcttgcc aaaggtacta aatgcggagt ttttagtttg
     2641 agacggggaa acccagcgaa tcgacctacg cgaagctgca ccttcatagc tttgatccct
     2701 catatttttt cttccgtttc gcgtgcctgt tgcagatttg ccgaagcgaa tcgcttcttc
     2761 catttttgaa tggaacccat gatccgacga cgcgttagcc gttcgaccgc gtgcaccatg
     2821 tgcggcgttt gcacgccctg gtctccatag tagaagcaag acttcggggg aagcgatggc
     2881 cgataatggt cagactgcgg tcgcttggga aatgctcagc agcgtggtgg cgatgagggc
     2941 aaaggcaagg gaaaacaagc tgcccaagct gttatcctca ttacatggag tcgaggtcga
     3001 gttggcgagg atcatcgctg gtacggaagc tccgcaggac gccaccatca acggcagcgt
     3061 acgaaatcaa ccggcagcca ttgccgccgc ggcggggtat atcgatgaaa cgtactccga
     3121 cgatacgggg ctggagccgg agcctccgcc ggcgccacgc tcgcggtctg gacgccgtgg
     3181 tggcggcccc cgcggtgatc aacaattgcg tgcgtatgcc aaaaagtatc tcgacatcac
     3241 gcggcagctg aaggagctgg tcgagcgagg cgaggcagcg gcgacgggaa atcatttcgg
     3301 ggatgcgatg gacgaggatt gggaagcgga atccgatgca ctgctcagcg atcagcatga
     3361 cctttccttc accatcgccg gcctgaaagc gacgaccttc gagggccttg tcgacaaggc
     3421 tgccgttctc gacgacctga tcgaggaaaa caccgatgat cccgtgcagg tcctcgcaag
     3481 gtcactggtc cgcgatgtca tcgcgatggg ccagaacggg aagtccggcc gctgaggaga
     3541 tatgacaaca ctggcggcat ttgctctttt cgcttttaca cggcaacgag acctcgtcgc
     3601 gcggtgcatt agtaaaatgt gcaccgccga cgaaggcttt atttcgaggt ttggcgcctt
     3661 tagccacgca aaaggaatcg aggttggctt tctttcgaag ccaaacgagg cgatttgagg
     3721 cgccgttgtg cttgtgccca tttgattgcc tttcgcgggc ggtagcgtgc tgttcttgcc
     3781 gatagttata tttttgaaat tttgatcatg tatgtgtccg ctcgctggcg gctgaaatgg
     3841 ggccgaatgc tgctcggaaa cgatgccgtt tccatcagtg tgcgaaacga gtttgtctga
     3901 ttcatatcgg ttaagagttc cggagcgctc cccgggaaat ccgcccttcc gaatagcagg
     3961 cgtgcggcat tttcagcatc cagtcgatgt aagtcccggt cgcgcggtct ccgagcgcca
     4021 gggtgaggtg gtagtgctcg aggcgctgag catgggatag ctgcattcgg gcccccggtg
     4081 cctgccgaag ttgagaaaca caggctgcca cgggcgcgat ggtgatcccg atggagccga
     4141 tctctgagcg cccggctggg gcatccattt ccgttgatag acttggcgcc gcctgttgcg
     4201 tggcggcggc tgccgctaca ctcgattccc atgaacgcgc caattcgcac accgagatcc
     4261 gccacaccgc tctcgcagcc gaagatcgcg ggagtgggct tcgtgcatct gaaggtccat
     4321 tcggcctatt cgctgctgga aggggcgctg cccatcggca agctggccaa gctgtgccag
     4381 gagcttgggt tccctgcagt ggcgcttacg gatacgaaca atctgttcgg tgtcctcgaa
     4441 ttttcggaca agctgagcag ctccggcatc cagccgatcg cgggcgtctc tctcgccatc
     4501 gacttcgagg aaaagaagcc cgcgcacatg ggcggcccgg cgtcgagctc ggatgctctg
     4561 gcgcggcgcg acggctcgat cgcgctctat gcgatgagcg agcgcgggta tgcgaacctg
     4621 atgaagctcg tgagccgcgc ccacctgcag gtcggtgaaa acgaggggcc acacattacc
     4681 atcagccagc tccggggcgc cgccgaggat ctgatcgtgc tgacgggcgg ccccgacgga
     4741 ccgatcgaca cggcgctgcg cgacggtcag aagacgctcg ccgaagcgcg tctcgaggcg
     4801 ctggcgagga tctgcggcga ccgcctttat gttgagattc agcgccacgg gctggagaag
     4861 gaggcgctgg tcgagccgga tctgctcgat ttggcctaca acctgaactt gcccctggtt
     4921 gcgacgaacg aagcatattt cgccagcccc gacgactacg aggcgcacga cgcattgctg
     4981 tgtatcgcgg agggccgtta cctcgtcgag gacgacaggc gtcgcgtttc gccggagcac
     5041 tacttcaaga gcgccgaaga gatgatggcg ctcttccagg atctgccgga agcgctcgac
     5101 aacaccatcg aaattgccaa gcgctgcgcc ttccggccga atgggcgcaa gccgatcctg
     5161 ccgcgcttcg ttgcgacctc ggaacgggcg agcgacgagg accagctcgc ggccgaagcg
     5221 gcggagctca agcgacaggc ggagcagggg ctgaaggcgc gccttgccgc caatccgctg
     5281 gcgccggggt tcaccgagga ggattactgg aagcggctcg ccttcgaggt cgacatcatc
     5341 acgcggatga agtttcccgg ctacttcctg atcgtggccg acttcatcaa gtgggcgaag
     5401 gcgcaaggca ttccggtcgg gccggggcgc ggatcgggcg cgggctcgct cgttgcctgg
     5461 tccctcacca tcaccgacct cgatccattg cgcttcgggc tgctgttcga gcgcttcctc
     5521 aatcccgagc gcgtctcgat gccggacttc gacatcgact tctgccagga ccggcgcgac
     5581 gaggtgatcc gctacgtcca gcggaagtac ggcgccgaca gggtggcgca gatcatcacg
     5641 cacggaaaac tgcaggcgcg cgccgtgctg cgcgacgtcg gacgcgtgct gcagatgccg
     5701 tacgggcagg tcgacaagct ctgcaagctg gtgccgaaca atccggccaa tccggtgagc
     5761 ctggccgaag ccatcgcagg cgagccgcgg ctgcaggagg cgcgtgactc ggatcctctc
     5821 gtggcgcgcc tgctcgagat cgcccagaag ctcgagggcc tctaccggca cgcctccacg
     5881 cacgccgccg gaatggtcat cggcgaccgg ccgctggatg agctggtgcc gctctatcgc
     5941 gatcccaaat cgaattttcc gatcacccag ttcaactgga agcaggtcga gaaggccggc
     6001 ctcgtcaagt ttgactttct gggcctcaag acgctcaccg tcctgaagaa ggccgtcgac
     6061 ctcatcgaac gttgccgagg cgtgaagatc gaccttgcat cccttccgct cgatgaccgg
     6121 cccacgtacg agatgctggc gcgggctgat actgtcggcg tgttccagct tgaaagcaca
     6181 gggatgcgcg aaagcctgaa gcggctcaaa cccgaccgct tcgaggacat catcgccatg
     6241 gtggcgctct atcgccctgg cccgatggac aacatcccga cctacatcaa ccgcaagcac
     6301 ggcgaggagc cggtcgactg cctgcacccc atcctggagc cgatcctcaa ggagacctac
     6361 ggcgtcatca tctaccagga gcaggtgatg cagatcgccc aggtgatggc cggctactcg
     6421 ctcggcgagg ccgacctgct gcggcgcgcc atgggcaaga aagacaaggc cgaaatggcc
     6481 aagcagcagg cccgtttcgt cgaaggcgcg gtcaagaacg gcgtcgccaa gaaggatgca
     6541 gtctacatct tcgagctggt cgacaagttc gcgggctacg gtttcaacaa gtcgcacgcg
     6601 gcggcctatg cgctggtcag ctatcacacg gcctacttga aggcgaacta tcgcgaggag
     6661 ttcctggcgg cgtcgatgac gctcgacatg ggcaatacgg acaagctcag cgtgttcgcg
     6721 gcggaggcac gccggagcgg catcgcgctg aagccgccgt gcgtgaacgc atcggctgtc
     6781 gatttcctgc ccgagccggc acgccgagaa ggagaggctg gcgccatccg ctattcgctc
     6841 gcggcgctga agaacatcgg ctcgggtgcc gtcgagtcga tcgtttcgac acggcagaaa
     6901 gacggaccgt tcaaggattt gtccgacttc gccagccggc tcgatacgaa ggcgctcaac
     6961 aagcgtgccc tcgagacgct ggccgccgct ggcgccttcg acgcgctcgg ggtagaccgc
     7021 gctctggttc atggcaacgt cgaccagatc atggccctcg cacatcgcat cgcgtccgat
     7081 gcggcgagcg gtatctccga cctgtttggc ggcggatcga gctcggcccc gcaactcgac
     7141 ctcaggcccg tcaagccgtg gacgccgatg gagctgctgg agaaggaatt cgaagccatc
     7201 ggctttttcc tgtccggcca tccgctcgac cagtacgaga gcgtgctgtc gcaactcggt
     7261 gtgcaaacct ggtcggaatt cgaagctgcc acggaacgcg gtgtttcggc gggccggctt
     7321 gcgggcgtcg tggtttccgc gcgcgaacgc aaatcgcaga aaggcaacaa attcgccttc
     7381 gccatgttct ccgattccac agggcagttc gaagcggtga ttttctccga cacgctcgct
     7441 caatcgcgcg atctcttgga gcctggcacg ccggttctgc tcaccgtcga ggccgaacgc
     7501 gaaggcgata cagtgaagat gcgcgtccag agcctcgaat cgctcgacaa agccgcaggc
     7561 gcggtgcagc gcggcttgaa gctcgtgatc ggcggcagcg cggtgcttgc gcgccgcgcc
     7621 gagatcgccg gcatccggga cctgctgaaa cccggccgcg gagaaattct gattcttctg
     7681 aaactcgacg agaaaggccg tgaggtcgag ttggccgtgc ctgggcgcta cgacgtcagc
     7741 ccgatggtga agggcacgct gtcgacagtg ccgggcgtgt tggaggtgcg tgagatttaa
     7801 gcgggcgaat agcgaaatag cgaattggat ttctgctccc ccattcgcta ttcgccattc
     7861 gccacttgct tcatatgaac cacgcgctgc gcaaacggcc tcgacagccc agccgcctcg
     7921 agctcgattt ttccacgctc cgtgaggctt cggcgaaccg gccagtaggt cgcggtggcc
     7981 gtccacacgc ggagagcgag aatcacggca ttgtcggcga gctcgtcgac gaagcagtcc
     8041 ggggccggat cagcgcgcac gcggtcatcc gcacgcgcca gagacagcaa cacctcgcgc
     8101 gcgcgatcta tgtcatccga atatgagatc gagaacttca ggtcgacgag acgctgctcc
     8161 aaacgcgagt aattgatgat gcgcttgctc cacagctccg aattcggaac gaactggtaa
     8221 atgccatccc acgaatggag ctcggaggcg aaaagcccga cctccttgac cgttccggcg
     8281 atatttccgg cttcgatgta gtcgccgacg cggaagggcc tcagccacag cagcatgatg
     8341 ccggcggcga tgttggcgag tgttccctgc aacgccaagc cgatcgcgag gccgatggcg
     8401 ccgagcgccg ccagtacgct tgtcgtctgg atgccgacct gtccgagggc cgccacgacg
     8461 acgacgatca ggacggcgta gcggaccagc gcggtcgtaa cgctccgcag cgtcatgtcc
     8521 attcgggctc ggtcgagcag tcgcccggtg ccgcgctccg cccagccggc aaccgcccag
     8581 cccacagcga gcatcaggag cgcgacggct acgttcggca gcacgccccc gagccacgcc
     8641 gccagagcct ccgtgaacgt gctgatcccg tcagtcatcg cgcccctccc gtcctaccgg
     8701 actcaccctt gtgagcgagc tctgccggtg aatagcatgc tgccggcacg ggcgggcaag
     8761 tcgggacgcg catcaagccg gggacgggag gggtcaggca ccgctggcga cgtgatccgc
     8821 ggcgcgcgca ccgcgctggc ccatggggcg aggcgcaccg ctcgtcgtat ccttgcgcgt
     8881 ttgcgcctcg gtctcggcgt tgatctcggc gccgatcagc acgatcgttg ccgacagcca
     8941 catccagacc atgaagccga cgaccgcgcc aagcgagccg tacgtcttgt tgtaagaccc
     9001 gaaattggaa acgtaccagg aaaacaggat cgagccggca agccacacga cagcagtgaa
     9061 aatgctgcct ggtgaaaccc agcgccattc cggattttcc cggctcgggc cgtagcggta
     9121 gagcagcgcc agcccgagga ggatggcgat cagaagcagc ggccagcgca caatggaaat
     9181 gatccaaccg gccatgctct gcaaaccaac gaacgccaac accgcgggca ggacgacgat
     9241 gccggtgacg gccaggatga tgaacaagat gccgcccaga gtgaacagca gcgcttggag
     9301 attgtactga atgaagctgc gccgctcggg ctcgtcgtag acgacgttca aggcttcgaa
     9361 tatcgatttg acgccggcat tggcgctcca gagcgagatg gccaggccga tgaagaaata
     9421 gaggccgagc gtgccgccgc cctgcgcctg aagctggctg atctggtcac cgatgatctg
     9481 caacgcgccc tcggggacaa cgccttccat attcgcgagc tgctgctgga tggcctccgg
     9541 gtctgcgacc aggccgaaga gcgagacgag tgcggcaagc gccgggaaca gggcgagcag
     9601 ggaatagtac gtcacgccgg ccgcgatcag catgaggcgg tgatcctgaa tgttccggta
     9661 gacgcgcacg gcgatgtctt tccagccatg ggccggaatc tcggaagggg tctccgcctc
     9721 gtggccacgg ccacccgaac tgcccgcctg ggcctgggat cccgccggct cgcctgggcg
     9781 agtcaccttg gcaacggctt tattgccggc gtgcgccggc ttcggctcac ccaatcgtct
     9841 gcgcagtgcc atagcagcca ggaccaggag cccaagcgca aggggcctgc gcttggggga
     9901 cgaagcgcct tctgcgccga aaaccgagcg ttgatgtgcc ttgtcgtctg ctgccatggt
     9961 agaagcgcaa cgtcgggacg ggcaggctcg ttccggcgga cgccgcagcc gggccttgca
    10021 atctcggcga acacccgcta aatcccgcct cattccacac gcggatcatc gcgctgcggc
    10081 ggattgaaag ccccgccggg cgctccggtg cgtttgccgc atcatcccaa gatcggcagc
    10141 gcttctcgat ccgcggaggt gtaaccggaa aacgagagat acgcatgctg ccaacgttca
    10201 ctatgcgcca gctgctcgaa gctggcgtgc acttcggtca ccaggcccac cgctggaacc
    10261 cgaagatggc gccgtacatc tacggcgtcc gcaataagat ccacatcatc gacctgacgc
    10321 agacggtgcc gctgctgcac caggccctgg tcaagatttc ggacacggtt gccgaggggg
    10381 gccgcgtcct gttcgtcggt acgaagcggc aggctgcaga cgccatcgcc gaggcggcca
    10441 agaggtcggc gcagtactac atcaatcacc gctggttggg cggcacgttg accaactgga
    10501 agacgatttc gcagtcgatc cgccgtctcc gccagctcga ggacatcctc gccgatccgc
    10561 agggccgcac gaagaaggag gtgctgaacc tcacccgcga gcgcgacaag ctcaatcagg
    10621 cgctgggcgg tatcaaggag atgggcggca cgcccgacct gctcttcgtg ctcgacacga
    10681 acaaggagca gatcgccatt caggaagcca agaagctcgg cattccgatc gtagcggtcg
    10741 tcgacacgaa ctgcgatccg gacggcatcg attacccgat cccgggcaac gacgatgccg
    10801 gccgcgcgat tactctctac tgcgacctgg ccgcccgtgc agcgatcgat ggcatccagc
    10861 gtgcccaggc agccgcgggc gtcgacatcg gcgcagccga ggcgccgccc gaagagcagc
    10921 ttccggccga gttcaagggc atcgaggcgc cgcgcgggga gccggacgat ctcaagcgca
    10981 tcagcgggat cacgccgaaa ctcgagcaac ggctcaacga cctcggcgtt ttccattttt
    11041 ggcagatcgc cgacctggac gccgacgccg tgcgctcact cgatcggcag ctgaagctca
    11101 agggccagat cgagcaggaa gactgggtcg gtcaggccaa gaggctcgtc gaggcggcag
    11161 ccgcctgacg cggggttcag cggcaaaagc cggaccccat cgcattcgtc ctgcaggcgc
    11221 cttcttgtgg gcctgctaat gcaacacgcg ggcgcgccgg tgcgggcctg ccaacccgag
    11281 agggtgacat gacgcagatt tcagccgcga tggtgaagga gctccgcgac aagacgggcg
    11341 cgggcatgat ggattgcaag caggcgctga tcgaaaacgc cggcgatatc gaggcgtcgg
    11401 tcgactggct gcgcaagaag ggcttgtcga aggcggccaa gaaatcgggc cgagtcgcgg
    11461 ccgagggcct gatcggcgtc gcagtcgaag gcaagacggg aacgctcgtc gaggtcaact
    11521 cggagaccga cttcgtcgcg cgcaacgcgc agttccagga gatggtgcgc gagatcagca
    11581 agctggcgct tcaggccggc ggcgatcttt cgaagctgct cgcgctgccg ttccccggca
    11641 agacggtatc cgtcgaagag cacgtcaggg agatgatcgc caccatcggc gagaacatga
    11701 acgtgcgccg cacggcacag ctcaccgcgg gtgcggacgg gatcatcgtc gattacatcc
    11761 acaaccgcgt cgccgagggc ctcggcaaga tgggtgtgct ggtggcgctc gagagcacgg
    11821 gcgacaaggc gaagctcaat gagttcggcc gccagatcgc gatgcatatc gctgcagcgg
    11881 cgcctctggc cattacggtc gaggacctcg acccagcagt gatcgagcgc gagcggaaga
    11941 tctacgagga gcaggcagcc gcgtccggca agccggctga catcgtggcc aagatggccg
    12001 aaggccgcct gcgcaaggag ttcttccagc aggtcgtgct gatgcagcaa atcttcgtga
    12061 tcgacggcaa gtcgactgtc gagcaggcgg taaaggaagc agcgggtacg gttggcgcgc
    12121 ctgtcaagat caccggcttc gtgcgctacg ccctcggtga aggcatcgag aagaaggaag
    12181 aggacttcgc cgccgaggtt gcggcggccg tcagcggcac gcggtaacgg tcggcgcgag
    12241 gcgacctgaa gttgacattg cggcctggtt cgccccgtgc ggccaggccg ttttgccgcc
    12301 tacttgccgc ggcgcgtgaa cagcgcactc attcccacca gcagccagcc gatgatcatg
    12361 gtcgatcctc cggttggcgc ggccatcgga aacagcccgc gatcaaggaa agcgcgcagc
    12421 ccgacgtcac ccgcaaacag aatggttccc agcgccagca gcgcggcgca gagcgcgccc
    12481 aaacgcagcc gccgtcccga aaggccgagg acgaggagag ccggtgcatt ggcgaggcag
    12541 atggcggaag ctgcaccaag gagtcgcacc tcggcgccgc tgtgtgcggc cgccgcggcc
    12601 gaggcgatgc ccgccgcgcc gatgaggccg gccaggacga ccgcccagcg gatgacgtgg
    12661 tcatgttggc cgtccgacaa cgctagtcct cctaccgcaa ttctccacga aaagattcag
    12721 gccttgcgcc agctcttgag ctccccgcgg cgcagcttgc cgttggcggt gcgggggagg
    12781 gcatcgagaa atatgatttc ccgcggacat ttataggggg cgagccgttc ggccgcgaac
    12841 tgctggatct cctgcgcgat gctgtcgccg cggcacgccg cctcgcgcag cacgacgaac
    12901 gcaccgatga cgctcacatc tgcgcgcacc tgcacctcgg cacaggcgac ctccgcgatg
    12961 gaaggatgct cggcgagcac ggcctcgacc tcctgtggcg ccacgcggta gccgagcgcc
    13021 ttcatgacat cgttggcgcg cccgcagtgc gtgacgtagc ccgcgccgtc catgcgggcg
    13081 agatcgccgc cgatgaacca gtccccgcgg agaacctcag cttcctcgtc gggtcggttc
    13141 caatatccga gcatcaggcc gggatcggta cgatgaaccg caagaaggcc ttgttctccg
    13201 gccggcagag gttcagtgcc accttctgcg ggcaggatca ccaccgatcg ccccggctgc
    13261 ggcttgccga cggcacccgg tttccgcggc acggatggcg aggaggaaat gtaggtcgac
    13321 agctcgctca tgccgagcgc ttcatacaac tcaagaccgg tgcgggcggt ccagttctcg
    13381 aataatcccg gcggcggagt ctcgccggcg atgaggccgt gccgcaaggc gccgagcgaa
    13441 ccgggagcga cgtccgtata gcgcaaaatc tgccggtaaa gcgtcgggac ggcagcgaag
    13501 atggtcgcct ccgcgctccg gatgaggcgc ggccacacgg ccggatcctt tggtcccgtg
    13561 tagacgatgg cggtcgcgcc gttggcccag ggatcggtga gacctgtgcc gagcgtgtag
    13621 gtccagttga aggcgcccgc gtgcagcacg cgatccgccg acgtgatgcc gtaccagccc
    13681 tgatacatgg gccggcgtcc ccatgcggcg cgatgggcgt ggagcacgcc cttcggcttg
    13741 gacgtcgtcc cggaggtgta aatgaggtag gcgggatcgt ctgccgagct gtcggcgtac
    13801 gttccggcaa ttggatgatc ggccagggcg cggacctctt caccgtccaa tacgagcacg
    13861 ccagccggga tgtcggagac cggcaatccg tccgcgagcg caatggcagc agcgccactg
    13921 tcctgcagca ggaatgccgc ctcccgctca gtgagctgcg atgccgttgg caaaggaacg
    13981 tatccgccgg cgatggcgcc gaagaagagc agggcgtagg tgctcgtgtt ctcgagccgg
    14041 atcataatgc gcgcgccggg ctccaggccc ttggccttca ggcctgcagc gatgcggagc
    14101 accgcgcttt cgatcttggc aaaggtccag acctcggctg gcggctggag cggatcggcg
    14161 atgacgatca gcgccggctt gtctggaaat gatttcgccg cacgtccgac ggcgtaagcc
    14221 gccatgttga agcggggtgg aggaggctcc ccccggtatg catggagctg ctgctccccc
    14281 ttcacggcgc ctctccggcc tgccaccacg tatcgaagct ggtaccaaag agagcggtct
    14341 ctttcggcgc gtgcaagtgc gaccagtgtg cgatccactg cttggggacg tggaacagcg
    14401 gaatgacata gtcgccggac aacagcacgc ggtcgaaggc gcgcaccgcg gtcgtcagct
    14461 catcgtacgt ccgtgccgcg agcagggcct cgatcatcgc gtccgcagcg gggctcttca
    14521 cgcccggata gttgaggctg ccttcgatgt cggccgcgcg cgatgaccag cggttgatct
    14581 gctcgttgcc gggtgagagc gaggcgcccc acgtccattg gatcatatcg aaatcgaacg
    14641 acttcaggcg cgcccagtac tgggccgcgt caacctgcct gatgtcgaca ttgatgccga
    14701 gccgctcgag gttcgtctgg aagctcagca tgatccgctc ctgcgcccgg ttggacgcga
    14761 ggaactcgaa cgacagctgt tcgccggtct tggcgttgac gagacggctg ccgtcgagct
    14821 tgtagcccgc ctccgtcagc agcttgtagg cctcacgcaa ggccgcgcga ttgttgccgg
    14881 atccatctcc gacaggcagg tgcgagcgcc cctccagcac gtccgggtcg acttcctcca
    14941 agtacggctc cagcagcttg cgttcggcct cggaggcggg gtgaccggtc gaggcgaggt
    15001 ccgagcggtc gaaatagctg gttgtgcgca catacaatcc atggaacagg ctgcggttga
    15061 tccactcagc gtcgaacatc aggatcaagg cgcgccgcac gcgctgatcc gcgaacacgg
    15121 gccgacgcgt gttgaagacg agcccgctca tgccggaggg cagccccgtc ttgaactcgc
    15181 ttttcaagac gcgcccgtcc gcgacggcct gaaagccgta gccttcggcc cagcgcgccg
    15241 ggtcttcctc cgcacggacg tcgatctgcc cggccttgaa ggcttcgaag aggcttgccg
    15301 catcgcgata gaaatcgatc cggatctcgt cgaagttata ccggccgcga ttgatcggca
    15361 gatccttgcc ccagtagttc ggatcccggc ggaacaccag gctgcgcccg gcttcgacgc
    15421 gatcgacgac gtaaggcccg ctgccgacgg gtggctccag actggtccga tcgaaagcct
    15481 ccgccgtcat gacatgcgcc ggcaggatcg gcatcaggcc gaggatcagc ggaatttccc
    15541 ggtcgccgcc ttccttgaag gtgaagcgta ccgcgtgcgg cccgatctgc tcggcgcgcg
    15601 ccaccttcga ataataggac cggtgatagg gccagccctt ttccttcagg agggcgtggg
    15661 agtggatgac gtcggcgggc gtgatcggcc gaccgtcgga aaagtgcgcc tccggccgca
    15721 ggtaaaaggt gatggagctg cggtcatcgg gcacttcgac ccgctcagcg atgagcccgt
    15781 aaagcgtgaa cggctcgtcc tgcgatcgcg ccatgaggct ttcgtagacg tactcgcgca
    15841 atccggatgg cgtcacgccc ttgatgatga aggggttcag gctgtcgaat gtgccgagcg
    15901 cccccatgcg gagccggccg cccttcggcg cgtcgggatt gacgtacgga agatgcgcga
    15961 agtcgggcgg cagggccggg gtgccgtgca tcgcgatgcc atgctgcccc acgctcgttc
    16021 cggcgacgag tgtcgctggg agcagcgccg ccatgagcac ggacgcgaga gctaggagca
    16081 gtttcactgg ccttccgttg ctgagcttgt cctggagcgg cagggctcag agcgcctcgc
    16141 cgccgcatat cgcagcccat taccatattt ggggcgcaat atgacccgaa cttctcctgc
    16201 gacgttgcga ctttgccacg ccgtccaggc gggctaggga gtgcacaggc gctcgggcag
    16261 gcggccacgg gattgccgca ttcgcaccat tagtccgccg ggcgcacgtc gcaaaggggg
    16321 gcgtgtcaac caggcgcggc acaggcgtcg agaaactagg gccgggcggg cacgggcccg
    16381 ctcacagcga ggtcgggaac agacgatggc gaacattgga tggttcggga gtggcgttgc
    16441 tcgcgccgcg gcgggcgtgg cagtggcgtt cggtctccag atggcgattt ccggtgctgc
    16501 tctggcccag caggggaaat cccagccgaa gcaggcccag caatctcaac cgaagcaggc
    16561 caagcaggag cagaagcaaa gtgcgtgggt caagctgtgc gagaaggccc agatcgcgca
    16621 catgggcaag gacggcaagc cggtcaggga agagaagaac atctgcctga cccaccatga
    16681 gcgcctggac ggcaataccg gcatggttct cgtatcggcc gccatccgcg atgtagaggg
    16741 catggacaaa gagcacctga tgatcatggt gccgctcggg atggccatcc cgccgggcat
    16801 ccgggctgcc gtctacacgc cggagcaatg ggagaaggcg cagaagaacg agaagatcga
    16861 cgagagcgag ctgaagccga tcagcctgca ttattcactc tgccatccag ccggctgcac
    16921 ggccgaggtg gaagccagca aggagctgat cgaatccatg aagaagggtg gtggactcat
    16981 ggtgctggcc atcaacgcga atggccagcc gatcgctttc cccgttcccc ttgacggctt
    17041 cactgccgcc cacagcggcc ctccggtcga caacaaggcc tatgccgagg cccgtagcca
    17101 gctcatgcag cagatccggc agcgtcaggc ggagctagtc aagcaggcgc aggagaagca
    17161 gaaagcggcc cagaaggaga aggctgccga gaagaagaag tagctctccg ctgggtcttg
    17221 ttcagccggt gcagcgcgtg agcgctgccc cctccctccc ggcgtagcgg caccatcgac
    17281 acaaggtcgc gctccgggct cgagctcgcg ggtgcgcctc tgccgtgcca ccctccctcc
    17341 gcgagtacgg ccaaggatta ccgaacggct cttggtctgc gaaaaccggc cgggccatgc
    17401 agcgcgaccg gctgacgtgg ccttccaact ggctcgcact caatgcggct gaacgaaggt
    17461 cgcctcgtac cgcccgttct cggcctcaga gaacagctcg agaatcttcg gatgccgtag
    17521 tggggcgcca ctgtcgtcca gcatcaagtt ctgctcggag acgtaggcga catactccgt
    17581 ctcggcattc tccgcgagca ggtggtagta gggctgatcc ttgcgcggtc ggatttcctc
    17641 ggggatcgac agccaccatt cctcggtgtt gtcgaatacg ggatccacat cgaagatgac
    17701 gccacggaac ggatagaacc gatggcggac cacctggccg atggcgtact ttgccacgct
    17761 gattttactc atggcggatt gggcaccata gctccccgga ctggcacccg gactcgggcg
    17821 tgcactggcg ccagcccccc catttcgtgc acccgatcga gaatagctca taaactcccc
    17881 cgtgctgttg cggcaagcgc acaacttgtc tcaaatcgcg tgaagcctcg tctcaaatac
    17941 cataggctga ggccagaccg ggatccttgg ccgcgacgag ccgcgcgaga tcgacgatca
    18001 cctttgcctg cttccaggtg gcgtcgtcct gcatcttacc gtcgatcatg acggcgcccg
    18061 ttccgtcagg catggcctcg aggatgcgtt ttgcaaacgc aacttcattc ggatcggggc
    18121 tgaacacgcg cttggcaatc gcaatctgct tcgggtgcag agaccacgct cccacacagc
    18181 ccatgatgaa ggcattgcgg aactggctct cgcaggcggc atcgtccgag aaatcaccga
    18241 acgggccgta gaacggcttg atcccgttgc gcacgcaggc atccaccatg cgcgcgacgg
    18301 tgtagtgcca cagatcctgc tggaagaagc cccgcggccc gcccgatccg gcatcggcga
    18361 gaacgccgta gtcgggatgg ccgccgccga cgcgggtcgt cttcatgccg cgggaggcgg
    18421 cgaggtcggc gggcccgagg ctcatgccgt gcatgcgcgg gctcgcagcg gcgatctcct
    18481 cgacgttgga aaccccttcg gccgtctcca gaatggcgtg gatcagaatg ggcctgtcga
    18541 tctggtgctt cgcttcgagc tgagccagaa gctggtcgag gtagtggatg tcccacggtc
    18601 cctcgacctt gggcagcatc atcacgtcca gcctggagcc cacggccgag acgatctcga
    18661 tgatgtcgtc gagtgcccac ggcgagttca aggcgttgat ccgcacccac agcccggtcg
    18721 tgccgaagtc gtgctgctgg gccatttcga tgaagccgcg ccgcgccgcc tccttgtcct
    18781 ccgcagggat ggcgtcctcc agattgccaa gcaggacgtc gacgctggcg atggtctccg
    18841 cgagacgcgc gcggatcttg gcattatgcg gcggcacgaa gtggatcatt ctttccagcc
    18901 gcaccggaag ctggcgcagc ggctccggcg caccgatggc gagcgggcta tagaacctgg
    18961 cgggcgtacg ggtcgacggc atggcacctc ctgcgctcgg gataatccgg cttcagtggg
    19021 gccatcgggt acgacggaag ggctgaggcc gcaaccctca actccagcgc cggttggacc
    19081 acatccattg ctcgggcgct tcccggatcc attcctcgaa ctggagctgc atctccgtca
    19141 tgatggtccg gatgtcgtcg gcctgattgg ccgttcgcgg caccttgagc tctctgatct
    19201 gcacccggaa acggctctcc gttccgatcc gcgcacagcg cgccagccac agacgggcgc
    19261 cgacccgccg ggcgatgatg gcggcgatgg cctgcgttcg cgcgggcttg ccgaagaacg
    19321 gcaccggaat gccggttcga tcgaagagat cgcagacgag cccgaggcgg ccgccgcgcc
    19381 tgacgtagtc ggtgatgatc cgcgccgtgc gctggctttc tccgtggtca ccttcgacac
    19441 ggccgcggcc gaacaaaccg ccgggataga gatccttccg ctgcgccctg agataccggt
    19501 cgacgtaagg gttgttgacc gagcgataaa tggcggccgg gttggcgcca gcgacgacga
    19561 gcggccagat ggcgaactcc caattgccca tgtgcaacga aacgccgatc gccgggccga
    19621 gcttgtcctt gtagcgggtg aacagtgcaa agttctcgat ctcgatccgc gaggggtcgc
    19681 gcaggatgcg gtcgatctgc atcgtctcgg ccatgacgcg gccgaggttt tcccaatgcc
    19741 ccatgacgat gtcgtggcgc tcggcctcgc tcttttccgg gaacgcgatg gccacgttct
    19801 cgagcgcccg ctggtgccgg acaggggaga cgatcggcgc gaggaagcgc cacgctttcg
    19861 ccgaagctct gattgccagg ctcagcggca atgcgcgtac cagcccgacg acggtccgga
    19921 gcacaacgta ctcggcccgg aactgcagat cgcgcaaggc cgcaggcacg cggattctcg
    19981 agcgctttct cttgtggacc ttcggcaccg ggcgtccgcc tgctgttttg accgaccggg
    20041 ttgatgccaa gctgcggcca cacgcgcaag gggcagcgcg ccgcaagccg tctgcaaaat
    20101 ggcgagtctg aagttcgctg gtgccgcgcg agggagttaa aggtaagtcg ttcagtaacc
    20161 gggcgagttg gaaagaggcg ggacgtgctc cgcgtaacgg acagcatcag cattcccgag
    20221 gtcgagatcg aggagcactt cgtccgcgcc tcgggaccgg gcggacagaa cgtgaacaag
    20281 ctctcgaccg cggtagagct acgtttcgat gtggcggcaa gcgagagctt gccgccgcct
    20341 gtgcgcgacc ggctccggaa gatcgcagga cgccgcatga cgaaggaggg cgtgctcgtc
    20401 attcgtgcgg agcgccaccg cactcaggag ctgaaccggg cagatgcgcg cgagcggctc
    20461 atcgcgctcg tacggcgggc gacgatgcag ccgaagcggc gaataccgac ccggccgaca
    20521 aaagcctcca gggagcggcg tctcgaaagc aaggttcggc ggggagagat caagcggcag
    20581 cggagcgcga aacccacgtt ggactagagg gcgaaaaatg accgccagaa gactttcggc
    20641 cacctgctgc atcgccggcg gcggccctgc ggggatcatg ctcggctttc tgctggcccg
    20701 ggccggcatc ccggtcgtcg tgctggaaaa gcacgccgat ttcctgcgcg atttccgcgg
    20761 cgataccatc cacccttcga cgctggagct gatggccgag ctcgggctcg acgatgcttt
    20821 cctgaaactg ccgcacgaga agctggtgcg tgtcagcggc cagttcggcg atcgtccctt
    20881 ggtgttcgcg gacttctcgc ggctgcccgt ccggcatccg tacaccgcga tcatgcctca
    20941 gtgggatttt ctgaatttcc tggctgagca ggggcggcgc tatccgggct tcacgctgct
    21001 catggagaca acggccgagg attttcttta tgagcgggac cgcatcgcgg gcgtgaaggc
    21061 gaacgcccca gacgggccgc tggagatcgt ggcggacctg gtcgtagcgg cagatgggcg
    21121 gcactcggtc ttacggtcgc gctcgggcct tcccctcgtc gacctcggcg ctccgatgga
    21181 cgtcctctgg ttccgcttgt cccggaggcc ggacgattcc cgcgaaacca tcggccgttt
    21241 cgcgccgggc cgactgctgg tccggatcaa tcgaggcgac tactggcagt gcgcgctggt
    21301 gatcccgaag ggcggcgccg acgatctgcg ccagcgtggc ctcgacgcgt tccgccgaca
    21361 ggtgggtgcc ttgctaccgt gggccgagag ccggatcacc ctcgacgtga gatcctggga
    21421 tgacgtcaag ctgctcgatg ttcgcgtcga ccggctgcgg cagtggtatc gatcgggcct
    21481 cctgctgatc ggcgatgcag cgcacgccat gtcgcccatc ggtggcgtcg gcatcaatct
    21541 tgccattcag gatgcggtcg cggcagcgaa catcctgtgg cagccgttgc gggacaagca
    21601 actcgaaacg catcacttgg cggccgtcca gcagcgccgg gaatggccga cccgagccac
    21661 gcagcagctg caagtcatcg tgcagaaccg tgtgatccgg ccggctctgt cggccgaggc
    21721 acccttacgg ccgccgcttc ttttgcgtct ggccgcgagc atccccatgc tgcgcggctt
    21781 gactgcgcgc ctggtcggac tggggcttcg gccggagcac attggatcac ccgagctgcc
    21841 gccggctctt tgatccatca actctcttta cgcgtctctg gagcgccggg cttatcggct
    21901 tcggaggtct tcgcctcgtc ccagagggca tccatctctt cgaggctcga gtcggccgga
    21961 cgctttcccc gtgcggccag cgcttcctcg atatagcgga agcgtcggac gaacttgtcg
    22021 ttggctgcgc gcagggccgc ctcgggatcg atcttcaggt ggcgggcaac gttcgcaatg
    22081 acgaacagca agtcgccgaa ctcctcgctg atcctgtctt cagcgccggc cgcaatggca
    22141 tcatcgagct ccgtcagctc ttccttgagc ttgtccatga cggggccgag gcccggccag
    22201 tcgaagccga cggctgcggc cctgtcctgc agcttcaccg cccgcgtcag tgcgggcagg
    22261 gggaggggga cgtcggccag gatactctcg gagcccgcgg agccctcgct ggaagctccg
    22321 gcgcgcgcct tctctgcccg ctcggcggcc ttggctcgct cccagaagtc ctccggcacg
    22381 gcggcggagc gggccgcctc gtcgccgaac acgtgaggat ggcggcggat catcttggtg
    22441 gtgatgccct cgacgacgtc gccgaagtcg aacgcctgct gctcctccgc catttgcgcg
    22501 tgatagacga cctggagcag caggtcgccg agctcctcgc gaagatgcgc taggtcgcca
    22561 cggctgatgg cgtctgcgac ctcgtaggcc tcctcgatgg tgtagggcgc gatcgtctcg
    22621 aagctctgct tcaggtccca cgggcagccg gtcaccggcg ttcgcaggcg gcgcatgatc
    22681 tgaagcagaa ctgcgatgtc gcgggacggt gatagggacg aggtcatcag cggattccgg
    22741 tcagcatgac tggcgccacc actagcacat gcggcccacg tgtgcgggcg tgcaattggc
    22801 ctcatcccaa tgaaaaagcc cggcctcgcg tgaggccggg ctgattctgg agatagcttt
    22861 ggcgggttac tgaccgaggt gcatcgttcc gcggcagaag gtgttgccaa gcttgatctg
    22921 gccctgctcc aggagaatgt cgttcgtgca gttcgcaagc gtctcgaacg ggatgccctg
    22981 ggcctgggtt ccgccaccag cgcccgtacc agagcccatg ccagaagtca tgccgccctg
    23041 aggctgaccg ctctgcatct gtggcgtctg ggtccagggc tgctgccctt gcccgagctg
    23101 gccgaactgc tgttggccct ggcctgtctg gccctgctgc cactgctgcc ggagttgacc
    23161 catacgctgg cgcgcttggc ccacccggct ggccacgtgc tgcgccatcg gccccgtcag
    23221 gatacctctc tcatgaacca cggtcttgac ccgcgccgtg tcaccgctcg gcgagatatc
    23281 gatgtctcgg acgtcgatgt tgatctgata attgctgacc agtttgcgcc cttgcagcat
    23341 gttggcagcg tagcccaggg catccgagac catctcatcg ctcgcctcgg caacggtcgt
    23401 ggcgacatgc cggtcgcgga cgaacaactg cgagatggtc gtgaattccg cgtcaggcgc
    23461 cacgtactgg ttgatatatt gcctcagagc gttgggatcc tgggtccgag ccgtctgctg
    23521 taggacattc tgagcctgct ggaagaagtt ttgaatgctc tgctgggtta gctgctggtg
    23581 gccctgcatc tgtcccgtca tgggttggcc gaactgctgg gtaccgaact gctgacccat
    23641 ctgctggcca ttgaactgct ggctctgctg agcctgcgca gcggtcgtga cggcgagagc
    23701 cgtgcccatt aagagggctg caaagttcac agtgttcctc acgacgcact cctgttgcat
    23761 gtttgctggt gccgccagaa cgtgaggcca attgccaagt tccgcgatgg tccggggtga
    23821 acgcgaaaaa agttgcatgc gctcctttgg cagcctgcca caaagagcag cgcgtgatcg
    23881 tccgatcgca ggaggtcgac gggactcgtc cgtgggccat taaccaagat gagccgcggg
    23941 aaagagcgac cgagttgcgg tgcgccggcg gccgcggcgc gacgaggcgt cggtgagccg
    24001 ccttcagctc accgatctca gcttacgggc ggatgacagg agaaggctgg gcgatgcgga
    24061 tccgcccagg gttggcgttc gtggggagcc ctgaggagtt tcccgcccac cgctctcatt
    24121 cgcataatct atattatgga aaatagagag aaatttgtga gacaactcaa agcgatacaa
    24181 taaagatcga cgcgccctaa gcccgggcgc gcgtcagccg ccctacttct ccgtacgctc
    24241 gtacttgtcc gcgatcatcc gatccagcag ccggacgccc cagccggaag cccagctctg
    24301 gctgatgtcg gtcttgggcg aatccatcgc gacacctgc
//
LOCUS       DASVDU010000109        36800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_999283, whole genome shotgun sequence.
ACCESSION   DASVDU010000109 DASVDU010000000
VERSION     DASVDU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 36800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36800
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999283"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VF226_06955"
     CDS             complement(<1..572)
                     /locus_tag="VF226_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEX7073765.1"
                     /translation="MPLDRKSIIVRGAREHNLKGIDLELPRDSLIVFTGLSGSGKSSL
                     AFDTIYAEGQRRYVESLSAYARQFLEMMQKPDVDHIEGLSPAISIEQKTTSKNPRSTV
                     GTVTEIYDYLRLLFARVGVPYSPVTGLPIESQTVSQMVDRVLALPEGTRLYLLAPIVR
                     GRKGEYRKELAELQKKGFQRVKINGTFYEIP"
     gene            773..1261
                     /locus_tag="VF226_06960"
     CDS             773..1261
                     /locus_tag="VF226_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073766.1"
                     /translation="MLTRDYKGADGIRLAALIFVALCLVPGGAHLASLLNKIDRAPEA
                     YMTMQQAYNGWALFGIVIYGALLTTALHTILVRRQRGPFWLSLGAFLLIVLTQVIFWA
                     FTYPANAVTSNWTVIPENFEGWRQQWEYSHAINAVLTFIAFVLLALSVIESRREEDAP
                     LA"
     gene            complement(1267..1899)
                     /locus_tag="VF226_06965"
     CDS             complement(1267..1899)
                     /locus_tag="VF226_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073767.1"
                     /translation="MNDLWPWLVLAGLGAFHGLNPAMGWLFAVALGLNRKSRSVVLLS
                     LLPIAIGHALSILIVAGAFVTAGILFDQRAVQIGAGLLLIGWAAYHQFRGHKHRIRVG
                     MRTGLMGLTLWSFLMATAHGAGLMLLPALMPLCLSGSPAREITAAGSAPIVLAAVAVH
                     TLSMLAVTGAVAVSVYEWIGLAILRSTWINLDLLWAAALAVTGLLLVALA"
     gene            complement(2015..3409)
                     /locus_tag="VF226_06970"
     CDS             complement(2015..3409)
                     /locus_tag="VF226_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding family protein"
                     /protein_id="HEX7073768.1"
                     /translation="MAIWKPDPSFYPSPRTAMKAPPEKLAYVVAFDPTGQHPDALSVI
                     DSDPASPTYAQLVSSVPMPNTRDELHHFGWNACSSCLCPNAPHPHTERRYLVVPGLRS
                     SRIHILDTKPDPRTPKIVKVIEPGEVAERAGYTRPHTIHCGPEGVYVSALANAEGGAP
                     GGIFLMDHDTFEILGRWEIDRGPQELAYDAWWHLGYDTLVTSEWGLPETFENGLIPEV
                     LLGAKYGRRLHFFDLHTRKHQQTIDFGDKYQLIFELRPAHDPTKAYGFVNCVLSLEDL
                     SSSIWVWHREGGRWAVTKIIDIPAEPADPDQLPPILKGFKAVPLFVTDIDLSMDDRFL
                     YVSCWGTGNLLQYDVSEPMKPRLTGKVRIGGVVSRATHPAANGKPLNGGPQMVEISRD
                     GKRVYFTNSLYGAVDDQLYPEGIEGWMVKIDADPEGGITFDEKFFVEWPKSHRPHQVR
                     LQGGDCSSDSYCYP"
     gene            complement(3574..3963)
                     /locus_tag="VF226_06975"
     CDS             complement(3574..3963)
                     /locus_tag="VF226_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7073769.1"
                     /translation="MRNNASVTYDCSKCPAYCCTYPVIVVTKRDVVRLAKHFGISPEE
                     AERKFTKSAHGHKRVLRRRADAHFGRACRFLDQKTRRCTVYAARPAVCRQFPAEKRCG
                     YYDFLEFERRHQNDPEFVATTDNGLWK"
     gene            4214..4987
                     /locus_tag="VF226_06980"
     CDS             4214..4987
                     /locus_tag="VF226_06980"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7073770.1"
                     /translation="MSNSLFDLAGRVAIVTGGNGGIGLGMATGLAEAGAAIVIAARDQ
                     QKSANAAEGLKQLGARVATVEVDVQDQDSVKRMVEAALDQFQRIDILVANAGINIRKP
                     PQDVKPEEWQAVLDVNLNGPFYCAQAVYPAMKAQGGGKIITIGSMTSIFGVPFAPAYS
                     ATKGGVVQLTRSLATAWAKDNIQVNCVLPGWIDTNLTRQARRQVEGLHERIEARTPAG
                     RWGDPADFAGIAVFLSSRASDFITGTAIPVDGGYSVSGG"
     gene            5171..7276
                     /locus_tag="VF226_06985"
     CDS             5171..7276
                     /locus_tag="VF226_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX7073771.1"
                     /translation="MPESARAAADNETPVNPYSLLEAVNSSSDTSHTAWLIFIAIMAY
                     LMVAVAGVTHKDLLLETAVTLPILQVNIQLTQFFRFAPILLVLFHLGLISQLVLLARK
                     ALEFDSAVRALEVSNRRTHPLRLELHNFFFVQAIAGPHRSIVMSAFLHGMSWLTLVVL
                     PVVLLLYIQISFLPYHDVAITWTHRIALGFDVLMLVLIGIFLTRAETSFFTAFWRTTL
                     HHPVSFVVTTSVLAIVSIFSFFIATIPGEELDRLTRAFLPQPAQDETVRGQRQVAGFS
                     LPYVAARADGTLWGIFHRNLAVTDEDLVSARGRVRLDEPSVSLRGRDLRYAVLDRTDL
                     NRVDFTGADLEGASFVGADLSNARLHCGDLSELILSEDRQRARCTSARNANFTRAKLT
                     GARMAGVDLTGATLEEADLESAELPYAVLAGANFSGARLEKADLTGGVAMPGANFLIA
                     SLQGADLTGAHAQGADFSSAGMQATVLSYARLQGAVLRDADLEGATLQRAKLLGTDLT
                     GAKLRAADLRGAAVWQAKSPSSGDLELTDLQSLNVSSPAESEIDELSAIIDGVKVPPV
                     KQLVSERLQPVLDADERRQWETSADRQQWHQLMLANASQDLDYPALLTEHLLRFMCHS
                     RWANGAVATGVAKRAQAPEFRGDIAAIHDRLKSKDCPASQTVAKKPVRELTVAVDSMR
                     LERVAPVTPIQAVTTRSAN"
     gene            7468..7962
                     /locus_tag="VF226_06990"
     CDS             7468..7962
                     /locus_tag="VF226_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7073772.1"
                     /translation="MAGSVNKVILVGNLGRDPEIRRTQDGRPIANLRIATSESWRDKS
                     TGERREKTEWHSVVIFNENLCKVAEQYLRKGSKVYIEGQLQTRKWQDQSGQERYTTEV
                     VLQGFNGTLTMLDGRAGGAGLQEGGQSDYGGDSGFGGYGESRRGGSKPSSSFDKPLDD
                     EIPF"
     gene            8398..11196
                     /gene="gyrA"
                     /locus_tag="VF226_06995"
     CDS             8398..11196
                     /gene="gyrA"
                     /locus_tag="VF226_06995"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7073773.1"
                     /translation="MTDTTGTPPAGGPSDIRPISVTEEMKRSYLDYAMSVIVSRALPD
                     VRDGLKPVHRRILYGMHELGLEWNKPYKKSARVVGDVIGKYHPHGDSAVYDALVRMAQ
                     DFSMSVPLVDGQGNFGSVDGDPPAAMRYTECRLARISQFLLDDLDKDTVDFQENYDGS
                     EREPSVLPAKFPNLLVNGAGGIAVGMATNIPPHNLGEVIDACLATLDDPEISLDALCQ
                     IVQGPDFPTGGLILGRAGALAAYHKGRGSIIMRAKVDIEEIRKDRNALIVTAIPYQVN
                     KRVLIERIADLVREKRIEGISDLWDESNRDGMRIVIELRRDAVPEVVLNQLYRYSDLQ
                     ATFGANMLALNGGRPEQLNLRDMITAFTSFREQVVSRRTKYLLAKARERAHVLVGLAI
                     AVANIDEVINLIRRAPSPPEAKEQLMARDWPAEEVAPFIALIADPRHTISEAGTYRLS
                     AEQAQAILDLRLQRLTALGRDEIGNDLKKLADQIKDYLEILSSRLRIVEIVKRELTEI
                     KEQFAVPRRTEIVELEGELEDEDLIQREDMVVTVSRRGYIKRVPLSTYRAQRRGGKGR
                     SGMTTRDEDFVTQLFIASTHTPVLFFSSRGMVYRMKVWRLPAASPQSVGKALINLLPL
                     EQDEVITSILPLPEDEKTWGDLQLMFATRSGNVRRNSLADFENIHRSGKIAMKLDEGD
                     RIVDVAICRPSDDVLLTTARGQCIRFPVEDVRVFKGRDSTGVRGIRLEEGDVVISMAI
                     LNHVEASPAERMAYLKQASAVRRGQTEEEEIAAVEPEAEAEAEAEPVDESAELTPERY
                     AELSAKEQFILTVSEHGFGKRSSSYEYRVSGRGGKGIAAMVVNERNGPLVASFPVEET
                     DQIMLVTNAGKLIRTSVDEVRIAGRNTQGVRIFRTDEDEKVVSVERIPEDSNGNGNGN
                     GNGNGNGNGSSENGAELT"
     gene            11303..12103
                     /locus_tag="VF226_07000"
     CDS             11303..12103
                     /locus_tag="VF226_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073774.1"
                     /translation="MRMLQALLVLVLFSTPGAVLAQNERVEEIATRGQSMRVLLTQPA
                     GPVGSVILIAGGHGNLALSAEGKIGWGAGNQLVRTRALYAKAGYLTATPDLAPDLKRG
                     KGVVPRYRWSDQHAADIGALVKHLRRIAAPVYLVGTSRGAISVINAAVKLSGEEAPDA
                     IVLTAGMLIHIDPKQPSAERNVGHLERVKQPVLMIYHEKDQCRYTPASSAERFAKLLT
                     SAKKVDISFLSGGSAVGDPCEANHYHGFRGLDAEVVRVITEWLGALKD"
     gene            12141..12764
                     /locus_tag="VF226_07005"
     CDS             12141..12764
                     /locus_tag="VF226_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7073775.1"
                     /translation="MSLHVFAAFAGACLLLALTPGPMMSLITANVAAHGVHAGLWTVA
                     GNLVSLSFMVAAAALGMTSLMVFMSDWFDVIRWIGALYLAWLGFERLKRAWQGESPTL
                     VPARSPSSRGWFLQAMAVGLSNPKVLLFLGAFLPQFVDTSGNIELQLSLLALTFVVVI
                     GLSDVTYAVALGRLRDLFAGGRARLLDGVSGTLLLLGGLVLATARRP"
     gene            12858..14585
                     /locus_tag="VF226_07010"
     CDS             12858..14585
                     /locus_tag="VF226_07010"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX7073776.1"
                     /translation="MSKPEDQAPREQGLLRGLQNYGDRDFSIYMRRAFVKSMGYSADE
                     LTRPVIGIAATSSQFNPCHRHFPELIEAVRRGVTAAGGLALTFPTISLHESTLAPNSM
                     KFRNLMAMDTEEMIRAQPMDAVVLLGGCDKTVPAQLMAAASAGKPAIQLIGGPQMTSR
                     YGDHRVGACTDCRKFWGKFRAGEIGKAEIEAVESRLVTTAGTCAVMGTASTMAAIAEA
                     LGMTLPGTAAIPAVHADRLRAAEASGAEAVRLVRSGLTPDKIITAKSVENAARVLLAL
                     GGSTNGVIHLAAIAGRLGISLDLRRFNELSDTTPVLAAVKPVGTEFYMEDFFAAGGIG
                     AIMRELKPLLHLDCLTVTGQTLGERLASEEGHYVDRRVIRPASDPLEPQGGLVALFGS
                     LAPDGAIIKRSAATPALFETEGRAVVFSSLEDMARRIDDPELDVRPDDILVLQNAGPR
                     SPYGMPEAGFLPIPKKLLAQGVKDMVRISDARMSGTAYGTIVLHVAPEAAAGGPLGLV
                     RSGDRIRLSVKERRLDLLVPEDELARRRAELASIEPELPERGYDRLFRETVLQAPQGC
                     DFDFLVPRR"
     gene            15129..16007
                     /locus_tag="VF226_07015"
     CDS             15129..16007
                     /locus_tag="VF226_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEX7073777.1"
                     /translation="MFRKPLVGLAAIAVSSCFALASHAQDIKLYAFSSGALTLGKGIL
                     QNLAPVDPPIQVPVGFYVITHPKGNVLFDTGNNDRIITDPSYWGAAFNDLKPINTPDV
                     AIDTQLEKIGLKPDDIKYVVVSHLHLDHGGNVGKFPNSTIVVQKDEILNAFWPQPGTG
                     GPYMIGDVMPLRAANSNYPNAVKMLQLEGDLDLYGDGTIVIKRWVAHTPGSQMMTVRL
                     KNTGLVILTGDNVYFRENVEKNLPPNIVLAYNPTGFLTAYEWIRYMMATEKADFFTAH
                     DPDAFKAMKKAPEFYD"
     gene            16118..16852
                     /locus_tag="VF226_07020"
     CDS             16118..16852
                     /locus_tag="VF226_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073778.1"
                     /translation="MAVLSLDRLSKRYGARLAVDSVSFNIEGREVFGLLGPNGSGKST
                     ILRLVTGYLRPTSGTVQVAGLDVARDGRAARRRIGYVPESAPLYGHMRVVEFLGFMGR
                     LRGIMGLALEKAVDAAVDRLALGDVRSVIIERLSYGYRQRVSLAQAILHEPELLVLDE
                     PTNGLDPRQIIELRGLLRRLAEDCAVLVTSHVLAEIERTADRVGILLDGRLLTIEPIT
                     REATEPPPPSRLETLFLDLTQAKAIQ"
     gene            16849..17550
                     /locus_tag="VF226_07025"
     CDS             16849..17550
                     /locus_tag="VF226_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073779.1"
                     /translation="MSVVGALAAKEIRTLFTSPIAYAVIAVFMVLSGYTFTVSLFVTK
                     QATLVHIFFQAAVQLILLVPLITMRQFAEERRTGTLELLLTAPVREIDIVLAKFVACM
                     VVLGVIAFSTLIYAVVLSVYGDPDWGPIYSGYVGLVLLGASLLSVGLTISALTANQIV
                     AAVVSLGLFGVLWSIDTLASLLPQPFENWLLGLSLLARFTPFAVGALYVSDFGFFLTV
                     ILLGLFLTVRALARS"
     gene            17554..19122
                     /locus_tag="VF226_07030"
     CDS             17554..19122
                     /locus_tag="VF226_07030"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEX7073780.1"
                     /translation="MGEHGLQLDALLWPAGWLIAVFVLFVSGLRLPLETRLSGLRAWL
                     YAASCVFAGLGVWVLANVALTLNDTHIDLTREKVYTPSADAMAVVDELRQPVRVTFFY
                     RSEDPIGRRAADILKVMGRRNPLLTVLNVDPDKQPELARREGVRLYNAAVVEAAGRRV
                     LIQSTSEAEIAIGIQRVLRERAITVCFLEGHGEFAMDGFEFHTHLEGLSDHSHGEASS
                     HVVETAGHGVGRLRRALEAQGYVARKLVLATSGAVPADCTLLIAANPRTTFLPAESAA
                     LRAYLASGGNALLLFDLGFVPEPGLAGLLSELGVRLEQQVVVDPLSHYQVDPEMVAVT
                     GYDPHPITNTVSLTFFPGIRPLTLTAPAPGVSVKPILQSSRDSYTRPVKPAGTRIIEG
                     SVSEAGSAAEEAIPIAAPVDFVADVRPRVLGIAAEGTLLPGAAPFRVVVIGDGDFASN
                     SFLPYMANSDLLLSAVRWLAREERGTAVRTRIPVPPMILLTAAQSRAIFGLIVILLPL
                     TVVGIGGIVWWRRR"
     gene            19149..19673
                     /locus_tag="VF226_07035"
     CDS             19149..19673
                     /locus_tag="VF226_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX7073781.1"
                     /translation="MPLIATLALAYLAVMVMVGAQPVQRQLVKFEAKGVLPFEPESVR
                     AILVGRDERKVALVRSGENGWVLKQGGAVEQGVATHLDNAVKMLHRSAPVREIAPEEL
                     TGVDTRPFGLENPSVVATLMGPAGQKLTVRFGALNPEGFLQYMRIDGDPKVYLMSRFI
                     GAEWSAALAGVEGR"
     gene            19670..20755
                     /locus_tag="VF226_07040"
     CDS             19670..20755
                     /locus_tag="VF226_07040"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX7073782.1"
                     /translation="MMQDARDGKPRSIGCWALGLLAGLWLACGIALAHTAGMTGYAKI
                     SIAGQTVRFAITFPTDALATDGSVRDLSDVADAVAQHVRITSDGTDCASVPPEVRPPA
                     PERASFDVVVHYACAVPVRNLEIRDGLDAVLGADYHTIADIEWPGGARRVVFEQDQRS
                     ASVSLADDAGEETQAAAGTLASYFALGVEHILLGLDHVLFVIALILPGGRLLSLAAMI
                     TAFTVAHSITLALSVLDIMTVPAWIVEPVIALSIAYVAFENLAMRSAVSRRWAVSFIF
                     GLVHGFGFAGALAEVGLPASALVAALVGFNLGVEAGQLLIIAVLLALLVWLQNSAWKP
                     RVAKASSIAIMAVGLALLTERVLTVSI"
     gene            21035..21757
                     /locus_tag="VF226_07045"
     CDS             21035..21757
                     /locus_tag="VF226_07045"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073783.1"
                     /translation="MPHDLIDVRSLSLARGSRMVLENLTFSVRRGDQVAVLGPSGIGK
                     STLLQIIAGLIAPSRGSVVIGGRPVKGPLPSVTMMLQRPALLPWATVLDNVLLGLRFS
                     GAVRRDAAAAKARAMGLLERVGLDDRADAKPAELSGGEQQRVALARALAPNPAVLLLD
                     EPFSALDPTTRKALRSDVAALARSSGVTLLLVTHDVADAAALCRRVIRLGGSPAGIIS
                     DETDIHASLELAQPGAAVLPAA"
     gene            21799..23034
                     /locus_tag="VF226_07050"
     CDS             21799..23034
                     /locus_tag="VF226_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073784.1"
                     /translation="MSKISDEDLRTLWAHEWTRADWSRRQSLDFLAKGGLAALLGTTG
                     LSRMAHASTDEVVRLGYLPITDAAALLVAHAKGFFEAEGLKVAKPTPVRSWSALVEGF
                     ASGKFNLVHLLKPIPVWMRYNNKFPVKVLAWAHTNGSGIVVGGNTGITDFKDLGGRRI
                     AVPYWYSMHNIVLQIALRQAGLRAVIKNDQPAADETALQILAPPEMPPALAAGKIDGY
                     IVAEPFNALGEVKVNARMLRFTGDIWKNHPCCVVTAHERDTVERPEWTQKLVNAVVKA
                     QIFASKNKAEVAKLISNEGTGAIPLPADIVHKAMIDYGAAYEATGAIQHRGWGNGRID
                     FQPWPYPSATKLIVKAMGETVVEGDTTFLDGLDPDFVTEDLVDYRFVKAALDREPDWT
                     LDPSVDKASPFVRSEVVKP"
     gene            23031..23900
                     /locus_tag="VF226_07055"
     CDS             23031..23900
                     /locus_tag="VF226_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073785.1"
                     /translation="MSESSFVLSARSPAVEDPPASGEPSREGDRPGSLRAMGQKIWPP
                     ALGIFGLALVWWLAGVVLEASPRLVAFTGFAPAPTLASFWQLLMSGDAWEAAAPSLYR
                     VLGGLGWGLALGAPVGLALGLVPLLERAVQLPFQILRMVSPLSWMPIAILAFPTWDGA
                     IVFLIAAAAIWPIMFATAAGVKRIDPAWLSVGRTLGGRGFPLLGRIVMPAVLPDLLMG
                     LRLALGIAWIILVPAEFLGVTSGLGYAINDARDTLSYDRLAALVLLIGLIGFVLDLAL
                     AVALKRLSWTPAA"
     gene            23925..24485
                     /locus_tag="VF226_07060"
     CDS             23925..24485
                     /locus_tag="VF226_07060"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7073786.1"
                     /translation="MAETLVTEFISVDPEGLAALSAKGKADPTVVRTVKCRTVAEGRR
                     FRHLNYIRDLPAHIVDEPKALLGDDTAPNPTEALLAALGTCLSVGIQANAVARGWKIN
                     GITLDLEGDINITSVWGAGDLSPKPVGLTAVRIAVDVDVEGASEEQIAELVEHSAKWS
                     PVVNTVKNPVAMEITPRRLDDDPRTR"
     gene            24491..25573
                     /locus_tag="VF226_07065"
     CDS             24491..25573
                     /locus_tag="VF226_07065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7073787.1"
                     /translation="MLNVLEPRGEAAAIRDIVASDIRPNLHAIDRDGVYPETAMRRLG
                     AAGAFSHHIGNSASDGGLDRAIAAMAEVGETCLSTAFCMWCQDALAWYLDRSDNDSLK
                     RRMLPAVASGTRLGGTGLSNPMKALAGIGRVALRGERVQGGYRVTGHLPWVSNLGDDH
                     SFGAIFALADGRLVMALIRCAADGVTLAQNAHFIALEGTRTFSVLLRDVFIPDGDIIA
                     EDATAFLPRIRQGFVLLQLGMALGAARGAASIMESDASGRSAAAWLPHPPEALRERAA
                     ALAKRASQLARSADDPSRSAFLDVLRLRLDGSWLALQATEAAALQFGARGYLKGSDVD
                     RRRREALFVALITPSIKHITQELAAD"
     gene            complement(25681..26865)
                     /locus_tag="VF226_07070"
     CDS             complement(25681..26865)
                     /locus_tag="VF226_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7073788.1"
                     /translation="MAGTASEGRTTRSAPLLRFVILYSLLYGSFGVASPYLPAFVETR
                     GLPAEQIGFIFAAGTAVRLVAAPLAGRIADHWQAQRLVFAACAVAAAVATLLYLPAST
                     FGIILLVNLAYAMALAPLTPLADALAVVAANRPRRGFEYGWVRGSGSAAFILGSIAAG
                     WSVASFGLAAILWWQAALLIAAAAVTRLVPALNAEEPSVSAVANEGLRTLWHSAAFRR
                     IVLVAALILGSHGMHDTFAMIRWTEAGIAPQVASLLWSLAVIAEVVVFFLVGPPLIRR
                     LTPAGSIALAAIVGAVRWAVAAISTDVWVLSLIQPMHGITFALLHLACMQLIARNVPE
                     DLEATAQAIYSTLGIGAATALVMLASGWLFGWLGGAAFALMSLLCLAALPLARGLRTS
                     RN"
     gene            27271..27609
                     /locus_tag="VF226_07075"
     CDS             27271..27609
                     /locus_tag="VF226_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073789.1"
                     /translation="MMRDGGRRLGSAWGGKRLLIALTVFCALESLLPGTALAEGAPPQ
                     NWESAYQDAAYFYSEGDLQQALSHAMRARRIIEQPGTDERDALAQVLSLEAVLLDRLG
                     VQSRREMESA"
     gene            27638..28627
                     /locus_tag="VF226_07080"
     CDS             27638..28627
                     /locus_tag="VF226_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007635327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX7073790.1"
                     /translation="MGQVLHGSAKTTHAIRAAIQRSKATIAELAEKYDLNPKTVMKWR
                     RRQSVEDMPMGPQNARSTVLSLEEEALCVAFRKHTLLALDDCLYALQASIPHLTRSSL
                     HRLFQRHGISRLPGIEGERTRKKFKAYPIGYFHIDIAEVRTAEGKLHMFVAIDRASKF
                     AFVELHDRATRRNASEFLRRLIKAVPYKVHTVLTDNGVQFTTPGNVASAARDIRDALD
                     RGELVWAHGFELVCAQNDIDHRLTRPNHPWTNGQVERMNRTLKEATIRRYYYESHDQL
                     RAHLAAFVAAYNFARRLKTLKGLTPFEHICKCWTEEPNRFIVDPTHLSPGLNT"
     gene            28558..31488
                     /locus_tag="VF226_07085"
     CDS             28558..31488
                     /locus_tag="VF226_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX7073791.1"
                     /translation="MLDRRAEPIHRRSNPPLSGTEHLGRHAESAPLHRRALKLREESL
                     HPEDPRLAESLNNLAVAVDLLGRHREAEALHKKALAIREKAFGPFHPDVAQSLNNLAK
                     VQLALGRPADAGPLLRRAISILSSGRGERLTRSQLDLADFSGTEREPDEGIAHVGKVG
                     AAMPGPAPVVRPRPAMRSAPAAPDSATADGMDRAPAEPADAALHEADLAVTLLNLGMM
                     AQTAYTGPKQEEAGLLYRRAIAIQEKVLGPEHPDLATSLNNLAELSLIQWRYREAEAL
                     HKRALAIREKALGPDHPLTAASLTNLARLYSLTRRPEIAAPLLVRAVAITEKALGPDH
                     PDTALAVRSLAMTHAALGDHTLALDFHRRASRSIIAHALSFAQGVDSDDSDAAQSRGR
                     LNHHFQSHIAALHRSVETGVEDEAVAREESFELAQRLGNSAAARALTQMTARVGRGPG
                     QLAQLVRERQDLRLRWKDADRRLLSALGAAASDRDESFIRSLREELAGLEKRIASVTE
                     VIDSAFPEYASLANPHPVPIGEAQQLLNEDEALIQFLIGSYGSYVWAVTKDNVRWVKL
                     AAGRRDIVRKVDALRCGLDARSWHDYGSQCQRLLETGYNLGDYERGAPLPFDLAKAHE
                     LYRALFGGIEDLIQDKHLLVIPSGPLTKLPLHVLVTASPPTRDRGSPADYSAVQWIVR
                     RQPMTIVPSVTALQALRSGASTKPPTRPFIGFGNPLLSGPDGQDRRAWQFQTCRREPS
                     LSFGQRLASALQYVPALSSLFRGSLADVRELRLQMPLPETADELCTIARRLGAPEPDV
                     WLGDRATEQNLKHINATGELARYGVLHFATHGLVAGDLTGLAEPALVLTPPNEASDED
                     DGLLTASEVAELELAADWVVLSACNTAAGQGGDAEALSGLARAFFHAGARALLVSHWE
                     VNSLAAVKLSTAAFAALADRRASGRAEAMRQAMLSMIESGEAFEAHPSNWAPFVVVGE
                     GGGG"
     gene            complement(32177..32617)
                     /locus_tag="VF226_07090"
     CDS             complement(32177..32617)
                     /locus_tag="VF226_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073792.1"
                     /translation="MRVRTLYLVVLATLMGGMLFAADVKAADKKTTPDKVKEFCGDNL
                     QSGGGAFGCTKCRPSKGPFKGGCIDYSCNYSGKGRQGCWKMVFKTVPPSGGKGTKAGS
                     NSVIGVNTIRAGSQRLRKVNRHPANKPTGVGGFATRSWKSKGAR"
     gene            complement(32770..33903)
                     /locus_tag="VF226_07095"
     CDS             complement(32770..33903)
                     /locus_tag="VF226_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7073793.1"
                     /translation="MKEDEKISALIGDIYDTVIDPATWPDVLRRTASFVGGQAAGLIA
                     RDTVNRSADASYSVGVDPHYLQLYLDQYARLDPTPTSFFFFDIGEVVCTVDLMPYEEF
                     QETRFFQEWARPQGWVDSVQAILEKSNTSFAHLSFLRNEATGLADEGTRRRMQLIVPH
                     VRRAVLIGKVIDLKTAEAASFADSLDGLSAGVFLIDARGRIVHANASGHDLLADGAVL
                     KAIDGKIVAGDPSAEQALCDVFLSASNGDAAIGSKGVAVPMAARSGEPYVAHLLPLTS
                     GARRGAGAPYSAVAALFVRRAALNIPSPPEVIARTYNLTPTELRVLMATVEVGGVPEV
                     AEALGVAETTVRFHLRRLFVKTGTHRQADLVKLVASFSNPLIR"
     gene            complement(34459..34621)
                     /locus_tag="VF226_07100"
                     /pseudo
     CDS             complement(34459..34621)
                     /locus_tag="VF226_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
     gene            complement(36070..>36800)
                     /locus_tag="VF226_07105"
     CDS             complement(36070..>36800)
                     /locus_tag="VF226_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073794.1"
                     /translation="PLTSIPAMKLPPATPPREDYWLTRKEVAERLRVARRMPQCKHIV
                     RALLIGVYTGTRPGAARKLRWIPSTSGGWFDLETETLHRRAQGEAENNKRKPKARIHR
                     RLLPWLRRWYRADMEIGIKRRISERGRRVERTVKTTYVIHYYGKPIEKLRRSWASVAI
                     AAGHAKQIGTDADGNPIWQIPDGPHVCRHTAATWLMQAGVDKREAAGYLGMSEKTLEE
                     VYGHHHPDFQSDAARADGRRRNRP"
BASE COUNT         6642 a        11562 c        11757 g         6839 t
ORIGIN      
        1 gggatctcgt agaaggtgcc gttgatcttg acgcgctgga agcccttctt ctgcagctcc
       61 gccagctcct tgcggtactc gcccttgcgg ccgcgcacga tgggcgcgag caggtaaagc
      121 cgcgtgccct cgggcagcgc cagcacgcga tcgaccatct ggctgaccgt ctggctctcg
      181 atgggcagcc cggtcacagg cgagtagggg acgccgacgc gcgcgaacag cagccgcagg
      241 tagtcgtaga tctctgtgac ggtgccgacg gtcgagcgtg ggttcttgga ggtggtcttc
      301 tgctcgatgg agatggctgg cgagaggccc tcgatgtgat ccacatcggg cttctgcatc
      361 atctccagga actgccgggc gtaggccgac aggctctcca cgtagcggcg ctgaccctcg
      421 gcatagatcg tgtcgaatgc gagcgaagat tttcccgagc cggaaagacc cgtgaatacg
      481 atcagggaat cgcgcggcag ctcgaggtcg atgcccttga ggttatgctc gcgcgcgccg
      541 cgtacgatga tgcttttgcg atcgagaggc atgcgggtgg gtgtagccga atgcggaatt
      601 ggcggcaatc gagccgtacc ggaacatagg tcggatgttg ggtgccggcc acgatcctaa
      661 aatgtgaccg gccgtcgccg tcgccgtatt ctcgacccgc gaaccagcta gccgctggcg
      721 cgttgatcga gacagataaa gtctcggcac ggagggatgg agaccggctc gcatgctgac
      781 gcgcgactac aagggcgctg atgggatcag gttggcggcc cttatcttcg tggccctgtg
      841 cctggtcccg ggaggcgccc atctggcgtc gttgctcaac aagatcgacc gtgcccccga
      901 ggcctacatg accatgcagc aagcctataa cggctgggcg ctgtttggca ttgtgatcta
      961 cggggcgctt ttgacgacgg cgttgcacac catcctcgtg cgacggcagc gtggtccgtt
     1021 ctggctgtcg ctcggcgcct tcctgctgat cgttctcacc caggtcatct tctgggcctt
     1081 cacgtatccg gccaacgcgg tgaccagcaa ctggaccgtt attccggaaa atttcgaggg
     1141 ctggcgccag cagtgggaat actcgcatgc aatcaatgcc gtcctgacgt tcatcgcatt
     1201 cgtgctgctg gcgctttccg tgatcgagag ccggcgcgag gaggacgcgc ctctcgcttg
     1261 acgggctcag gcgagcgcga cgagcagcag tcccgtcacg gccagcgccg cagcccagag
     1321 cagatcgagg ttgatccagg tgctgcggag gatggcaagc ccgatccatt cgtagacgct
     1381 gacagcgacg gcgccggtta cggccagcat cgacaacgta tggacggcga cggcagcgag
     1441 aacgatcggg gccgagccgg cggcggtaat ctctctcgcg ggcgagccgg aaaggcacag
     1501 cggcattagg gccgggagca gcatcagccc cgcgccatgc gccgtcgcca tcaggaacga
     1561 ccagagcgtg agccccatca ggccggtccg catgccgacc cggatccggt gcttgtgccc
     1621 gcggaattga tgataggccg cccagccgat caggaggagg cccgcaccga tctggacagc
     1681 gcgctggtcg aacaggatgc ctgccgtcac aaaggcgccg gccacgatca ggatcgacag
     1741 tgcatgccca atcgcgatcg gcagcagcga cagcagcacg acggaacggc tcttccggtt
     1801 gagccccagt gccaccgcga acagccagcc catggcgggg ttgaggccgt ggaaggcgcc
     1861 gagtcccgcg agcacgagcc agggccacag gtcattcatg cgcaccggac tgtgggactt
     1921 ggggcgcctg gaccatgatg cagatcaatt tggctccctg aaaaggtcgc accggtccag
     1981 gcgtggcggc agcgagccgc cacgcgctac cccgtcacgg atagcagtag gaatccgatg
     2041 agcagtcgcc gccctggagc cggacctgat gcggccggtg cgacttcggc cattcgacga
     2101 agaacttctc gtcgaacgtg atgccgcctt cggggtcggc atcgatcttc accatccagc
     2161 cctcgatccc ttccggataa agctggtcgt caacggctcc gtagagcgag ttcgtgaaat
     2221 agacccgctt cccgtcgcgg ctgatctcga ccatttgcgg gccgccgttg agcggcttgc
     2281 cattggcggc gggatgagtc gcgcgcgaga cgacacctcc gatccgcacc ttgccggtca
     2341 atctcggctt catcggctcg gaaacatcgt actgcagcag attgcccgtt ccccagcagg
     2401 acacatagag aaacctgtcg tccatcgaca ggtcgatgtc cgtcacgaac agcggtacgg
     2461 ccttgaagcc cttgaggatg ggagggagtt ggtcggggtc ggccggctca gcgggaatgt
     2521 cgatgatctt ggtcactgcc catctgccgc cctcacgatg ccacacccag atcgatgagg
     2581 agagatcctc aaggctcagc acacagttga cgaacccgta ggccttggtc gggtcgtgcg
     2641 ccggcctcag ctcgaagatg agctgatact tgtcgccgaa atcgatggtc tgctggtgct
     2701 tgcgggtatg caggtcaaag aagtgcagcc gacgcccata tttcgcgccg agcaggacct
     2761 ccgggatgag cccgttctcg aacgtctcgg gcaaacccca ttcgctggtg acgagcgtat
     2821 cgtagccgag gtgccaccag gcgtcatagg cgagctcctg cggcccgcgg tcgatctccc
     2881 atcgtccgag gatctcgaag gtgtcatgat ccatcaggaa gatgccgccg ggtgctccgc
     2941 cctcggcatt ggcgagcgcc gagacataga cgccctccgg gccgcaatgg atggtgtggg
     3001 ggcgcgtgta gccggcgcgc tcggcgacct cgcctggctc gatcaccttc acgatcttag
     3061 gggttctggg atcgggcttg gtgtcgagaa tgtggatgcg tgaggagcgc agcccaggca
     3121 cgacgagata ccggcgctca gtatgcggat gtggagcgtt ggggcagaga caggacgagc
     3181 acgcgttcca gccgaaatga tgcagctcgt cgcgggtgtt gggcatgggg acgctgctga
     3241 cgagctgcgc gtaggtcggt gaggccgggt cgctgtcgat gaccgagagg gcatcgggat
     3301 gctgacccgt cggatcgaac gcgaccacat aggccagctt ctccggcggc gctttcatcg
     3361 ccgtgcgggg cgatggatag aatgagggat cgggcttcca gatggccatg tcatcacctt
     3421 gccgaaggcg ctaaggcccc gcgcgcaaat caacgcggca gcaagggtgg cggatccctg
     3481 aactcgcgat cgcggccctc gggggtcgtg cgtgcgcgtt ggcgggagga aggagtgaca
     3541 gctcagacgt gtcgcgtgcg gctgcgtcga cggctacttc cacagcccat tgtcggtcgt
     3601 ggcgacgaac tccgggtcgt tctggtggcg gcgctcgaat tcgagaaaat catagtagcc
     3661 gcagcgcttc tctgccggga actggcggca gactgcggga cgcgcggcgt aaacggtgca
     3721 gcggcgcgtc ttctgatcca ggaagcggca cgctctgccg aaatgcgcat cggcccggcg
     3781 gcgcaacacg cgcttatggc cgtgcgcaga cttggtgaac ttccgctcgg cctcctcggg
     3841 tgagatgccg aagtgcttcg cgagccgcac cacgtcccgc ttcgtcacga cgatgacggg
     3901 ataggtacag caataggccg ggcacttgct gcaatcgtag gtcacggaag cattgttgcg
     3961 catttcggcg ggccatgctg atgcagacgc attccagttg cctactgcga ttgtgcttct
     4021 cttgaaatcc ccgctgcaga aaaaaccgcg gggtccaacg caaagacaca cttcgcccgc
     4081 aggcggctgg gtgcgctccc cgctgaaggg gcgcgtcgcc caatgttgcg aaagagatgc
     4141 cgcccaatgt tgcgaacttc tcgatcatca cactagggtg cgtccatgag gagaccagct
     4201 cgggagcact aggatgagca attcactgtt cgatcttgcc ggccgcgtgg ccatcgtcac
     4261 aggtggcaat ggcgggatcg gtctcgggat ggcaacggga ctggctgagg ccggcgcggc
     4321 catcgtgatc gccgcgcgcg atcagcagaa gtcggctaac gccgccgaag gcctgaagca
     4381 gctcggcgcc cgcgtcgcga cggtcgaggt cgatgtccag gaccaggact cggtgaagcg
     4441 catggttgag gcggcgctcg accagttcca gcgcatcgat atcctcgtcg ccaatgccgg
     4501 aataaacatt cgcaagccgc cgcaggacgt aaagcctgag gaatggcagg cggtgctcga
     4561 tgtgaacctc aacgggccgt tctactgcgc ccaggccgtc taccccgcca tgaaggcgca
     4621 aggcggcggc aagatcatca ccatcggctc gatgacgtcg atcttcggcg ttccctttgc
     4681 gcctgcgtat tccgccacca agggcggcgt cgtccagctc acccggtcac tggcgaccgc
     4741 ctgggcgaag gacaacattc aggtcaactg cgtgctgccg ggctggatcg acaccaacct
     4801 gacgcgccag gcgcgcaggc aggtcgaggg gctgcacgag cgcatcgagg cccggacgcc
     4861 ggcgggccgg tggggtgacc cggccgactt tgccggcatc gccgtgttcc tctcgagccg
     4921 cgcatccgat ttcatcacgg gcacggctat tcctgtggat ggcggatatt cggtaagcgg
     4981 cggataggct gcgcgagacc gtgaactcgt gacatcgtga agttgtggat cgtgcggttc
     5041 cagtgagggc tttcacgatc tgacgacttc acgatctcac gctttcaccc cctcccgagt
     5101 ggcccccttg ccacagattt tccgctatgg tctgcacctc tctttcccga gttcatcagt
     5161 ggggggtgca atgccggaat cagctcgtgc tgcggcggac aacgagacgc cggtcaaccc
     5221 gtacagcctc ctggaagcgg tcaacagctc gagcgatacc tcgcacaccg cgtggctgat
     5281 cttcatcgcc atcatggcct acctcatggt cgcggtggcc ggggtgaccc acaaggacct
     5341 gctgctcgag acggccgtca cgctgccgat cctgcaggtg aacatccagc tcacgcagtt
     5401 cttccgcttt gctccgatcc tgctggtcct gtttcatctg gggctgatct cgcagctcgt
     5461 cctgctggct cgcaaggcgc tggagttcga ctcagcggtg cgggccctcg aagtgagcaa
     5521 ccggcgcacg catcccctgc gtctggagct gcacaatttc tttttcgtgc aggccatcgc
     5581 cggtccgcat cgcagcatcg tgatgagcgc ttttctgcac ggcatgtctt ggctgacgct
     5641 ggtcgtcctg ccggtcgtgc tgctgctcta catccagatc tcgttcctgc cctaccatga
     5701 cgtggcgatc acctggacgc accgcatcgc gctgggcttc gacgtcctga tgctggtgct
     5761 gatcggcatc ttcttgacgc gggccgagac atcgttcttc acggcgttct ggcgcacgac
     5821 gctgcaccat ccggtgagct tcgtggtcac caccagcgtg ctggccatcg tgtccatctt
     5881 ctcattcttc atcgccacga tcccgggcga agagctggac cgcctgacgc gcgccttcct
     5941 cccccagcca gcccaggatg agacggtgcg cgggcagcgc caggttgcgg gcttcagcct
     6001 cccctacgtc gccgcccgtg ccgatgggac gctgtggggg attttccatc gcaatctcgc
     6061 cgtgaccgac gaggatctgg tcagcgcacg cgggcgggtc cggctcgacg agccgagcgt
     6121 cagcctgcgc ggacgtgacc taaggtacgc cgtgctcgat cgtacggacc tcaaccgggt
     6181 cgacttcacg ggcgcagatc tggagggcgc cagcttcgtc ggtgccgatc tcagcaacgc
     6241 ccgcctgcac tgcggagacc tttcggagct gatcctgagc gaggatcgcc agcgcgcacg
     6301 ctgcaccagt gcccgcaatg ccaattttac ccgtgccaag ctgacgggcg cccgcatggc
     6361 cggcgtcgac cttacggggg cgacgctcga ggaggcggat ctcgaaagcg cagagctgcc
     6421 gtacgccgtg cttgccggtg ccaacttctc cggtgcccgg ttggagaagg cggacctgac
     6481 cggcggggtc gccatgccgg gagcgaactt tctgatcgcg tcgttgcagg gggccgacct
     6541 cacgggcgcg cacgcgcagg gagcggactt ctcgagcgcc ggtatgcagg ccaccgtgct
     6601 gagctatgcc cgcctgcagg gggctgtctt gcgggatgcg gatctggaag gcgccacgct
     6661 gcagcgggcg aagctgctgg gcacggacct gaccggagcc aagctgcgtg cagcagatct
     6721 gcgaggagcg gctgtctggc aggccaagtc accttcaagc ggcgacctcg agctgaccga
     6781 tcttcaatcc ctcaatgtat cttcaccggc cgagagcgag attgacgagc tgtcggcaat
     6841 catcgatgga gtcaaggtgc ctccggtgaa gcagcttgtg agtgagcggc tgcaacccgt
     6901 gctggatgcc gatgagcggc gtcagtggga gacatcggcg gaccggcaac agtggcatca
     6961 gctgatgctc gccaacgcca gccaggacct cgactatccg gccctgctga ccgagcatct
     7021 gctgcgcttc atgtgtcact cgcgttgggc gaatggtgct gtggccaccg gcgtcgccaa
     7081 gcgggcccag gctccggagt tccgaggcga tattgcggcc atccacgatc gcttgaagtc
     7141 aaaggactgt ccggcgtccc agacggtcgc caagaagccg gtgcgggagc tcaccgtcgc
     7201 agtcgacagc atgcggctgg agcgggtggc tccggttaca cccatccagg cggtcacgac
     7261 caggtcggcg aattagcagg tgtgttgccc ccgcggtcct tgcgccggca gtctgcctgc
     7321 attattttgg aacgaaatga gaacgtgaat tccgtggaca ggtgactgcg ccgcccactc
     7381 catggatagg ttgatgcaac gcgaatcgtg catgtcggtc tgcgatcgtt ggcaggtcag
     7441 acgcggcgta acagcaagga gcttacgatg gctggatccg tgaacaaggt gatcctagtc
     7501 ggcaatctcg gcagggatcc cgagatccgc cgcacgcagg acgggcggcc gatcgccaat
     7561 ctcaggatcg ccaccagcga gagctggcgc gacaagtcca ccggcgagcg ccgcgagaag
     7621 accgagtggc attccgtcgt gatcttcaac gagaacctgt gcaaggtcgc cgagcaatac
     7681 ctgcgcaagg gctccaaggt ttacatcgag gggcagctgc agacgcgcaa atggcaggac
     7741 cagtcgggcc aggagcgcta cacgaccgag gtcgtgctgc agggcttcaa cggcacgctc
     7801 accatgctcg acggccgcgc gggcggcgcc gggctgcagg aaggcggcca gtccgattac
     7861 ggcggcgatt ccggcttcgg cggttacggg gagtcccgcc gtggtggctc caagccgtcc
     7921 agcagcttcg acaagccgct ggatgacgag attccgttct gaaccggggc cgagccgctc
     7981 actccccttc ggcgatgcga tagccgaggc aacgccgctc tggcgacagc catgaccgga
     8041 atgcccgtgg taagcatcac ggatttccgc cttcgcggaa atgacggttg gggcgggtgt
     8101 caggtgcgca atccgcgggg gctctggttt tattcccgtc gccgctgcgg tggcggggta
     8161 tccacgatgc gataccacgg ctgtgagttg ccacccgtcg gaatggccag caatgaggtc
     8221 gctgaggggt tccgctcaag ctcaagaatc cccatatttc atacgtgcga atagaggccc
     8281 cacgggcccc gagttgcggg tcggtgccca gcaggagtat aacttgcacg gcctgaattc
     8341 ttcggaacgc acgcccatgg aggcgtgcgt ttccgctgtc tatgaacgga tatcaccttg
     8401 accgatacga ctggaacacc ccctgccggc gggccgagcg acattcgccc gatctctgtc
     8461 acggaggaga tgaagcgctc gtatctcgat tacgcgatga gcgtgatcgt gagccgtgcg
     8521 ttgcccgacg tgcgcgacgg cctgaagccg gtgcaccgcc gcatcctcta cggcatgcac
     8581 gagctggggc tggagtggaa caagccttac aagaagtccg cccgcgtggt gggcgacgtc
     8641 atcggcaagt atcatccgca cggcgacagc gccgtctacg acgccctggt gcgcatggct
     8701 caggacttct ccatgagcgt gccgctcgtc gacgggcagg ggaacttcgg ctccgtcgat
     8761 ggcgacccgc cagcggccat gcggtacacc gagtgccgtc tcgcccgcat cagccagttc
     8821 ctgctcgacg acctcgacaa ggacaccgtc gacttccagg agaactacga cggctccgag
     8881 cgcgagccga gcgtgctgcc cgcgaagttc ccaaacctgc ttgtcaatgg cgcgggcggc
     8941 atcgccgtcg gcatggcgac gaacatccca ccgcacaacc tgggcgaggt gatcgacgcc
     9001 tgcctggcca cgcttgacga ccccgagatc agcctcgatg cgctctgcca gatcgtgcag
     9061 ggtcctgact tcccgaccgg cggcttgatc cttggccggg ccggcgcgct cgccgcctac
     9121 cacaagggcc gcggctccat catcatgcgc gccaaggtcg acatcgagga gatccgcaag
     9181 gaccgcaacg cacttatcgt caccgccatt ccctatcagg tgaacaagcg cgtgctgatc
     9241 gagcgcatcg ccgatctcgt ccgcgagaag cgcatcgagg gcatctcgga cctctgggac
     9301 gagtcgaacc gcgatggcat gcgcatcgtc atcgagctga ggcgcgatgc ggtgcccgag
     9361 gtcgtgctca accagctcta ccgctactcc gacctgcagg cgaccttcgg cgccaacatg
     9421 ctggcgctca acggcggccg gcctgagcag ctcaacctgc gggacatgat cacggcgttc
     9481 accagcttcc gtgagcaggt ggtgagccgc cggacgaagt acttgctggc gaaggcccgc
     9541 gagcgcgctc acgtgttggt tggcctcgcc atcgccgttg ccaacatcga cgaggtcatc
     9601 aatctgatcc ggcgcgcgcc ctcaccgccc gaggccaagg agcagctgat ggcgcgggac
     9661 tggccggctg aggaggtcgc gccgttcatc gcgctgatcg ccgatccgcg gcacacgatc
     9721 tccgaggcgg gcacgtatcg gctgagtgcc gagcaggccc aggccatcct cgacctgcgc
     9781 ctgcagcgtc tgacggcgct gggccgggac gagatcggca acgacctcaa gaagctcgcc
     9841 gaccagatca aggattacct cgagatcctt tcctcgcggc tccgcatcgt cgagatcgtc
     9901 aaacgggagc tgacggagat caaggagcag ttcgccgtgc cgcggcggac cgagatcgtc
     9961 gaattggaag gcgagctcga ggacgaggac ctgatccagc gcgaggacat ggtcgtcacg
    10021 gtcagccggc gcggctacat caagcgggtg ccgctgtcga cctatcgggc gcagcgccgg
    10081 ggcggcaagg gccgctccgg catgacgacg cgcgatgagg acttcgtgac ccagctcttc
    10141 atcgcctcga cgcacacgcc cgtgctgttc ttctcatcgc gcggcatggt ctaccggatg
    10201 aaggtgtggc gcttgccagc ggccagtccg caatcggtgg gcaaggcgct catcaacctg
    10261 ctgccgctcg agcaggacga ggtcatcacc tcgatcctcc cgctccctga ggatgagaag
    10321 acctggggcg atctgcaact gatgttcgcg acgcgctccg gcaacgtccg gcgcaactcg
    10381 ctcgccgatt tcgagaacat ccaccgcagc ggcaagatcg cgatgaagtt ggacgagggc
    10441 gaccgaattg tggatgtcgc catctgccgc ccgagcgacg acgtgctgct gacgacggcg
    10501 cgcggccagt gcatccgctt cccggtcgag gacgtgcgcg tgttcaaagg ccgcgattcc
    10561 actggcgtgc ggggcatccg gctggaggag ggcgacgtcg tcatctcgat ggccattctg
    10621 aaccacgtcg aagcctcgcc ggccgagcgc atggcctacc tgaagcaggc aagcgccgtg
    10681 cgccgcgggc agaccgagga ggaggagatc gcagccgtcg agcccgaggc tgaagctgaa
    10741 gccgaggccg agccggtcga cgagtcggcc gagctcactc cggaacggta tgcggagctt
    10801 tcggccaagg agcagttcat cctcaccgtc tcggagcacg gttttggcaa gcgctcgtcc
    10861 tcctacgagt accgggtatc cggccgcggc ggcaaaggca tcgcagccat ggtcgtcaac
    10921 gagcgcaacg gtccgctggt ggcctccttc cccgtggagg agaccgatca gatcatgctg
    10981 gtcacgaatg ccggcaagct gatccgcacc tccgtggacg aggtgcgtat cgccggtcgc
    11041 aatacccagg gcgtgcgcat cttccgcacc gacgaggacg agaaggtcgt ctccgtcgaa
    11101 cgcattcccg aggatagcaa cgggaatggc aacggaaatg gcaatggcaa tggcaatggc
    11161 aatgggtcca gcgagaacgg cgcggaattg acatagcctt cgcgctctga ctactgaatt
    11221 ctcctgattg gcttggaacg gcgcgggtga acccgtgccg tttttcatgc aaacgcgcgt
    11281 agctgatcag gggaggaaga tgatgcgtat gctgcaagcc ttgctggttc tggtgctttt
    11341 cagcacccca ggtgccgttc tcgcgcagaa cgagagggtg gaggagatcg ccacgcgcgg
    11401 ccagtcgatg cgcgttctcc tgacccagcc tgccggcccg gttggcagcg tcatcctgat
    11461 tgccggcgga cacgggaatc tggcgctgtc tgccgaaggc aagattggct ggggtgccgg
    11521 caaccagctc gtccggaccc gggcgcttta tgccaaggcc gggtacctca ccgcgacacc
    11581 tgatctcgcg cccgatctga agcgaggcaa aggcgtcgtt cctagatacc gttggtcgga
    11641 tcaacatgca gccgacatcg gggcgctcgt gaagcactta cggcgcattg ctgcgccggt
    11701 ctatctcgtc ggcacgagcc ggggcgccat ttccgtcatc aatgccgccg tgaagctgtc
    11761 aggcgaggag gcccccgatg ctatcgtcct cacagccggc atgttgatcc atatcgatcc
    11821 gaagcagcca tcggccgaga ggaatgtcgg ccacctcgaa cgtgttaagc agccggtgct
    11881 gatgatctat catgagaagg atcaatgcag atatacgccg gcttcctcgg ccgaacggtt
    11941 cgcaaaactg ttgacctcag ccaagaaggt cgacatcagc tttctgtccg gaggaagtgc
    12001 agtcggcgac ccgtgcgaag cgaaccacta tcacgggttt cgcgggctcg atgccgaggt
    12061 cgttcgcgtc ataacggaat ggctcggcgc gctgaaggac tgagctgtag cgttttttgt
    12121 ggcaggcagg agatcaggtg atgtcactgc acgtgttcgc cgcgttcgcc ggggcctgct
    12181 tgctgcttgc gttgacaccc gggccgatga tgtcgctgat cacggccaac gtggcggccc
    12241 atggcgtgca cgcgggttta tggaccgttg ctggcaatct cgtcagcctg tcgttcatgg
    12301 tcgcggctgc cgctctcggc atgacctcgc tcatggtctt catgtccgat tggttcgatg
    12361 tcatccgctg gatcggcgcg ctttatctcg cctggctcgg gttcgagcgg ttgaagcggg
    12421 cttggcaagg agaatcgcca acgctcgtgc cggcgcgcag cccgtcgagc cgtggctggt
    12481 tcctgcaggc catggccgtc ggtctcagca acccgaaggt gctgcttttc ctgggagcct
    12541 tcctgcccca gttcgtcgac accagcggca atatcgagct gcagctttcg ttgctggcgc
    12601 tgacgttcgt ggtcgtgatc ggcctttcgg acgtgactta cgccgtcgca ttgggccggc
    12661 ttcgcgatct ctttgccggc ggccgcgcgc gccttctcga cggcgtttcc gggacactcc
    12721 tgctgctcgg cggactcgtg ctcgcgacgg cacggcggcc ctgaccaaag gcccgcgcct
    12781 gacagccaag tctttggcgc gcatgctgtg tcctgctatg gttcggcaaa cgaacgccaa
    12841 gcggggagcc gagcgcaatg tcgaagcctg aggaccaagc accacgcgag cagggcctgt
    12901 tgcgtggcct gcagaactat ggcgatcgcg atttctcgat ctacatgcgc cgggccttcg
    12961 tcaagtcgat gggctattcg gccgatgagc tcacgcggcc ggtcatcggc attgcggcca
    13021 cgtcgagcca gttcaatccc tgccaccgcc attttcccga gctgatcgag gccgtccggc
    13081 gcggcgtgac ggcggcgggc ggcctggcgc tgaccttccc gaccatctcg ctgcacgagt
    13141 cgacgctggc gccgaactcg atgaagttcc gcaacctcat ggccatggac acggaagaga
    13201 tgatccgtgc tcagccgatg gatgcggtgg tgctgctcgg cggttgcgac aagaccgtgc
    13261 cggcgcagct gatggcggcg gcgagcgcgg gaaaacctgc gatccagctc atcggtgggc
    13321 cgcagatgac ctcgcgctat ggcgaccacc gggtaggtgc gtgtacggac tgccgtaagt
    13381 tctggggcaa attccgcgcc ggcgagatcg gcaaggcgga gatcgaggcc gtcgaaagcc
    13441 ggctcgtcac cacggcggga acctgtgccg tcatggggac ggcttcgacc atggcggcca
    13501 tcgcggaggc cctcggcatg acgctgcccg gcacggcggc gatcccggcc gtccacgccg
    13561 accggctgcg ggccgcggag gcgagcggtg cggaggccgt gcgtctcgtg cgctctgggc
    13621 tgacgccgga caagatcatc acggccaagt cggtcgagaa cgccgcgcgc gttctgctgg
    13681 ctctgggcgg ctcgacgaac ggcgtcatcc atctcgcggc gatcgcgggc cgcctcggca
    13741 tttcgctcga cctgcggcgc ttcaacgagc tctccgatac caccccggtg ctggcggccg
    13801 taaagccggt cgggaccgaa ttctacatgg aggatttctt cgccgcgggc ggcatcggcg
    13861 ccattatgcg cgaattgaag ccgcttttgc atctcgattg cctgacagtc acgggtcaga
    13921 cgctcggcga gcggcttgct tcggaggagg gtcattatgt cgatcgccgc gtgatccgac
    13981 cggccagcga tcctcttgag cctcagggcg ggctcgttgc gctcttcggc tcgctggcgc
    14041 cggatggcgc catcatcaag cgttcggcgg cgacaccggc tctgttcgag acggaaggcc
    14101 gggccgtggt gttcagctcg ctcgaggaca tggcccgccg catcgacgat cccgagctcg
    14161 acgtgcgtcc ggacgacatc ctggtgctgc agaacgctgg gccgcgctca ccatacggaa
    14221 tgccggaggc aggtttcctg cccatcccga agaagctgct cgcccaggga gtcaaggaca
    14281 tggtgcgcat ttcggatgcg cgcatgtcgg gtaccgccta cggcacgatc gttctgcacg
    14341 ttgctccgga agcggcggca ggcggtccac tcggtctcgt ccgcagcgga gatcgcatcc
    14401 ggctgagcgt caaggaacgc cggctcgatc tgctcgttcc ggaggacgag ctggcccgcc
    14461 gccgcgccga actggcgagc atcgaacccg aattgcctga gcgcggctat gacaggctgt
    14521 ttcgcgagac ggttttgcag gcgccgcagg gctgcgactt cgactttctg gttccgcggc
    14581 gataaacggt gattttcgaa agatgcgatt gaagtgacca ctggctgcaa aaaatgcgat
    14641 gccgcggcgg aagctcactc cgagactgaa cagctagatc atttcgaaat aggttgaaac
    14701 aagccagcta aggttatgcg ccgtctccct cgcccaagtg gggagaggtg aaggggccaa
    14761 ttgccggccg tttccacctc attcgaaaga gctatagagt cgacactgcc tatgcctttt
    14821 tcggcccttg tcgctgcgca aggcagaagg gggaagcatc gatgacgggt ttgcggccgg
    14881 cgatcaaccg gccagttgtc ggcgatccgc atctcactcg cgacgggcgg tgccaaacct
    14941 tccaaagaac ccgcggtcaa ctccaccgag gcaaggctgg cgaacgggac atttatcgtc
    15001 aaaaaagtct ttactcggag gtccttgaca cggaagcagg cgcgggtgtt aactcttgag
    15061 ataacaacta atggttgcat tcgcgatcga aatttcccaa acagcaccgc cagttccggg
    15121 aggatcagat gttccgcaaa ccgctggtag gactcgcggc aatcgccgtg agcagttgct
    15181 tcgccctggc gtcgcatgcg caagacatca agttgtacgc cttcagttcc ggcgccctta
    15241 ccctgggcaa gggtatccta cagaacttgg ctccggtcga tccgccgatc caggtcccgg
    15301 tcggctttta cgtcatcacg catcccaagg ggaatgtgct gttcgacacc ggcaacaatg
    15361 atcgcatcat cacggatccg agctattggg gtgccgcatt caacgacctg aaacccataa
    15421 acacgccgga cgtggcgatc gacacgcaac tcgagaagat cggcctcaaa cccgacgaca
    15481 ttaagtatgt cgtcgtcagc catctgcatc tcgatcacgg cggcaatgtc ggcaagttcc
    15541 ccaattcgac gattgtcgtt cagaaggatg aaatcctgaa cgcattctgg cctcaaccgg
    15601 gaactggggg cccctatatg atcggcgacg tcatgccgct cagggcagcc aacagcaact
    15661 atccgaacgc cgtgaaaatg ctgcagctcg agggtgacct cgatctgtat ggagatggaa
    15721 ccattgtgat caagcgttgg gtcgcccata cacccggcag ccagatgatg accgtaaggc
    15781 tgaaaaatac cggccttgtc atattaaccg gcgacaacgt ctatttccgc gaaaacgtgg
    15841 aaaaaaacct cccgccgaat atcgtgctgg cctacaatcc gacgggcttt cttaccgcgt
    15901 atgaatggat tcgctacatg atggccacgg agaaagccga tttctttacg gcgcacgatc
    15961 cggatgcttt caaggcaatg aagaaagcgc cagagttcta cgactgatgc gctgagagtg
    16021 aagccatccg cgtttgcgcg aaatccacgg gaagctaata agacgaggcc acgaatagtg
    16081 gcctcctact ttcactgaaa gcacgagtga tttgcacgtg gccgtccttt cgctcgaccg
    16141 gctgagcaag cgttatggcg cacggctggc ggtggattca gtctccttca acatcgaggg
    16201 acgcgaagtc ttcggcctgc ttgggcccaa tggctccggc aagagcacga tcttgaggct
    16261 cgtcaccggc tatcttcggc cgacttccgg gaccgtgcag gtcgctggcc tcgatgtcgc
    16321 tcgagatggc cgtgccgcac ggaggcgcat cggctatgtg ccggagagtg ccccgctcta
    16381 tggtcatatg cgcgtcgtgg agttcctagg gttcatgggg cgactgcgcg ggattatggg
    16441 tctcgcgctc gaaaaggccg tcgacgctgc ggtcgatcgg ctggcactcg gggatgtgcg
    16501 cagtgtgatc atcgagcggc tgtcgtatgg ctatcggcag cgcgtgtcgc ttgcacaggc
    16561 catcttgcac gagccggagc tgcttgttct cgacgagccg accaacggcc tcgatccgcg
    16621 gcagatcatc gaattgcgtg ggctgctgag gcgccttgcc gaggactgtg ccgttctggt
    16681 gacctctcac gtcctggcgg agatcgagcg cacggccgac cgtgtcggca ttctgctcga
    16741 tggtcgcctg ctgacgatcg agccgatcac ccgagaagcg acagaaccgc ccccgccatc
    16801 gcgcctcgaa acactgttct tggacctcac ccaagcaaag gcgatccaat gagcgttgtc
    16861 ggagcgctgg cggcaaaaga aatccgcacc ctttttacct cgccgatcgc ctacgcggtg
    16921 atcgcggtgt tcatggttct gagcggctac acgtttaccg tatcactgtt cgtcaccaag
    16981 caggcgacgc tcgtgcacat cttctttcag gcggccgtcc agctgatcct gctggtcccg
    17041 ctgatcacca tgcgtcagtt cgccgaggag cggcggaccg gcacgctcga gctgcttctg
    17101 acggcgccgg tccgggaaat cgacattgtc ctcgcaaagt tcgtggcctg catggtggtt
    17161 ttgggggtga tagccttctc gaccctgatc tacgcggtcg tgctctcggt ctatggcgat
    17221 ccagactggg gtccgatcta cagcggctat gtcggccttg tcttgctggg tgcgtccctt
    17281 ctctcggtcg ggctcacgat ttcggcgctg accgccaatc agatcgtcgc ggcggtcgtc
    17341 tcactcgggc tcttcggcgt tctgtggtcc atcgatacgc tggcgtcgct cttgccgcag
    17401 ccgttcgaga attggctgct cggcctgtcg ctgctcgcgc gtttcacgcc gtttgcggtc
    17461 ggcgcgttgt acgtatccga ttttggattt tttctcaccg ttatcctgct cggcctgttt
    17521 ttgactgtac gggcgctggc gcggagctga gcgatgggtg agcatggcct ccagcttgat
    17581 gcgctgttat ggccggccgg atggctcatt gccgtctttg tgcttttcgt ctccggcctc
    17641 cggcttccgc tcgagacacg cctgagcggc ctgcgggcat ggctctatgc cgcgagctgc
    17701 gtcttcgctg ggcttggcgt ctgggtgctg gcgaacgtcg cactgacgct caacgacacg
    17761 cacatcgatc taacgcgaga gaaggtgtac acgccttcag ccgacgccat ggctgtggtc
    17821 gacgagctga ggcagccggt tcgcgttacg tttttctacc ggtcggagga tccgatcggg
    17881 cgacgcgccg ccgacatcct gaaggtgatg ggccgacgca atccgctgct caccgtcctc
    17941 aacgtcgatc ccgacaagca gcccgagctc gcacgccgcg agggcgttcg actgtacaac
    18001 gccgctgtcg tcgaggccgc cggccggcgc gttctcattc aaagcaccag cgaggcggag
    18061 atcgccatcg ggatccagcg ggtgttgcgc gagcgggcca tcaccgtctg cttcctcgag
    18121 ggccatggcg agttcgcaat ggacggcttc gagttccaca ctcatctcga gggtctcagc
    18181 gatcacagcc acggtgaagc ctcctcacac gtcgtcgaaa cggccgggca cggcgtgggc
    18241 cgcttgcgtc gggccctcga agctcagggc tatgtcgctc gcaagctggt gctggcgaca
    18301 agcggtgcgg tccctgccga ttgcacgctg ctcattgctg caaatccgcg taccaccttc
    18361 ctgccggccg aaagcgcagc ccttcgggcc tatctggcca gcggaggcaa tgccttgctg
    18421 ctgttcgacc tcggtttcgt cccggagccg ggtctggcgg ggctgctctc agagctcggc
    18481 gtccggctcg agcagcaggt cgtcgtcgat ccactgagcc attaccaagt cgatcctgag
    18541 atggtggccg tgaccggcta tgatccccac ccgatcacca atacggtgtc gctgaccttt
    18601 ttccccggaa tccggcccct gacgctgacc gcaccggcac cgggcgtgag cgtcaaaccg
    18661 atcctgcaaa gcagccgcga cagctacaca cggcccgtga agcctgccgg cacgcggata
    18721 atcgaaggtt ccgtctcaga ggcaggcagt gccgccgaag aggcgatccc gattgcggct
    18781 ccggtcgact tcgtcgcgga tgtgaggccg cgcgtgctgg gcatcgccgc cgagggaaca
    18841 ctgctgccgg gcgcagcgcc gttccgggtc gtggtgatcg gggatggcga cttcgccagc
    18901 aattccttcc tgccgtacat ggccaatagt gatctgctgc tgtctgccgt gcggtggctc
    18961 gctcgcgagg agcgtggcac ggccgttcga acgcgaatac ccgtgccacc gatgatcctg
    19021 ctcacggccg cgcagagccg tgcaatcttt ggactgatcg tcattctgct gccgctgacc
    19081 gtcgtcggca tcggcggcat cgtctggtgg cggcggaggt gagagacatg acgccatcac
    19141 gcgttgtcat gccgctcatc gccacgctgg cgttggctta tctggccgtc atggtgatgg
    19201 tcggtgcgca accggtacag cgccaactgg tgaagttcga ggcgaagggc gtcctgccgt
    19261 tcgagcccga gtcggttcgc gcgatcttgg tcgggcgcga tgaacgcaaa gtggccctgg
    19321 tgcgcagcgg cgaaaatggc tgggtgctga aacagggcgg ggcggtcgag cagggcgtcg
    19381 caacacacct cgataatgca gtgaaaatgc tgcaccgctc ggcgccggtc cgcgagatcg
    19441 cccccgaaga gctaacgggt gtcgatacgc gtccattcgg cctcgaaaat ccgtcggtcg
    19501 tggcaacgct catggggccg gccggtcaaa agctgacggt acgattcgga gcgctcaatc
    19561 ccgagggttt cctccaatac atgcggatcg acggtgaccc caaggtctat ctgatgtcgc
    19621 gcttcattgg tgccgaatgg agcgcagcgc tggccggcgt ggaggggcga tgatgcaaga
    19681 cgcaagggac ggcaaaccgc gttcgatcgg ctgttgggca ctcggcttgc ttgcaggcct
    19741 gtggctggct tgcggcatcg ccctcgccca cacggcggga atgaccggtt acgccaagat
    19801 ttccattgct ggccagaccg tgcgctttgc catcacgttt ccgacggacg cattggcaac
    19861 tgacggttcg gtgcgggacc tgagcgatgt cgcagacgcg gtggcgcagc acgttaggat
    19921 tacgtctgac ggtacggatt gcgccagcgt ccccccagag gtgcggccgc cggcgcctga
    19981 acgcgccagc ttcgatgtcg tggtgcatta tgcgtgtgcg gtgccggtga gaaatctcga
    20041 gatccgtgat ggcctcgatg ccgtgctcgg cgccgactac catacgatcg ccgacatcga
    20101 atggcccggc ggcgcaaggc gggtggtgtt cgagcaggat caacgctcgg cgagcgtttc
    20161 gcttgccgac gatgcagggg aggaaacaca ggcagcggcg ggcacgctcg cgtcctattt
    20221 cgcgctcggc gtggagcaca tcctgctcgg cctcgaccac gtgttgtttg tcatcgctct
    20281 cattctgcca ggcgggcggc tgctttcgct ggccgcgatg atcaccgcct tcacagtggc
    20341 ccacagcatc acgctggcgc tctcggtcct cgatatcatg accgttcccg cttggatcgt
    20401 cgaaccggtg attgcgctgt cgatcgccta tgtcgccttc gagaacctcg cgatgaggag
    20461 tgcagtgtcg cggcgctggg cggtcagctt cattttcggg ctggtgcacg gcttcggctt
    20521 tgctggcgca cttgccgaag tgggcctgcc agcgagcgca ttggtggccg cgctcgtcgg
    20581 gttcaacctg ggtgtcgaag ccgggcagct gctgatcatc gccgtgcttc tggcgcttct
    20641 cgtatggctc cagaattctg cctggaagcc gcgagtggcc aaagcttcgt cgatcgccat
    20701 catggctgtc ggactcgctc tgttgaccga gcgcgtgctc actgtgtcca tctgatcggc
    20761 tcgcgccctc tgcgctggcg ccgcgaactc tgcccacatg ctgcgctttg cgactgctcc
    20821 atcagcccgg tagggccttt gtgctgacct gaacgcaatt ttaccgcgct cccgcaaact
    20881 gcaacgggag gatacgatcc aacgggctgc tgtataaatc attctattga attacctgat
    20941 agagaattag tttcctaagt ttgttgatta tgctggcgtc ttagggaata tcattctatt
    21001 tttgatccag tcgcgcttgc cactgggtca cgccatgcct cacgatttga tcgatgtccg
    21061 ctccctaagc ctcgcacgcg gcagccggat ggtgctcgag aacctgacct tcagtgtcag
    21121 gcgtggcgat caggtcgcgg tgctcgggcc ctccgggatt ggcaagtcga cgcttctgca
    21181 gatcattgcc gggctcattg ctccgtcgcg cggctccgtg gtgatcggcg gccggccggt
    21241 gaagggtccg ctgccgagcg tgacgatgat gctgcagcgg ccggcgctgc tgccatgggc
    21301 gacggtgctc gacaatgtcc tgcttgggct gcgcttctcg ggcgccgtcc ggcgggatgc
    21361 ggccgcagcc aaggcacgag ccatgggcct cttggagcgc gtcggacttg atgaccgcgc
    21421 cgacgccaaa ccggccgagc tgtctggcgg cgagcagcag cgcgtggcgc ttgcgcgggc
    21481 gctcgctcca aaccctgctg ttttgctgct cgatgagccg ttctcggcgc ttgatccgac
    21541 gacccgaaag gccctgcggt ccgatgtagc tgcgctggcc cggagcagcg gcgtgacgct
    21601 gcttctcgtg acccatgatg tcgccgatgc cgccgcgctg tgccgccgcg tcatccgcct
    21661 cggtgggagc cctgccggca tcatcagcga cgagacggat atccacgcaa gcctggagct
    21721 cgcccaaccc ggcgccgccg tgctgccggc ggcctaagcg gcagaaaagc cagactccca
    21781 acaatcgaga ggtcgatcat gagcaagatt tccgacgagg acttgcggac gctttgggcc
    21841 cacgagtgga cgcgtgccga ctggtcgcgc cgccagtcgc tggatttcct ggccaagggc
    21901 ggccttgccg cgctgctggg gacgaccggc ctttcgcgca tggcacacgc ctcgacagac
    21961 gaggtcgtgc gcctcgggta cctgccgatc acggatgcgg cggccctgct ggtcgctcat
    22021 gccaagggct tcttcgaggc tgaagggctg aaggtcgcga agccgacgcc ggtacggtcg
    22081 tggtcggcat tggtcgaagg gttcgcctcc ggcaaattca atctcgttca cctgctgaag
    22141 ccgatcccgg tctggatgcg ctacaacaac aagttccccg tgaaggtgct ggcctgggcg
    22201 cacacgaatg gctcgggcat tgtggtcggc ggcaatacgg gcatcactga tttcaaggac
    22261 ctgggcggcc ggcgcatcgc ggtgccctac tggtactcga tgcataacat cgtgctgcag
    22321 atcgccctgc ggcaggcggg cctgcgggcg gtgatcaaga acgatcagcc ggctgcagac
    22381 gagacggcat tgcagatcct cgcaccgcca gagatgccgc ccgcactcgc cgccggcaag
    22441 atcgatggct acatcgtcgc cgaaccgttc aatgcgctgg gagaggtgaa ggtgaacgcg
    22501 cgcatgctgc gcttcaccgg agacatctgg aaaaatcatc cgtgctgcgt cgtcaccgcc
    22561 catgaacggg acaccgtaga gcggccggaa tggacgcaga agctcgtcaa cgccgtcgtg
    22621 aaagcgcaga tcttcgcctc gaagaacaag gcggaggtcg cgaagctgat ctccaatgaa
    22681 ggcaccggcg cgataccgct gccggccgac atcgtccaca aggcgatgat cgattacggc
    22741 gctgcctacg aggcaaccgg cgccatccag caccgcggct ggggcaacgg ccgcatcgac
    22801 ttccagccgt ggccctatcc gtcggcaacg aagctcatcg tcaaggcgat gggcgagacg
    22861 gtggtggagg gggatacgac cttcctcgac gggctcgatc ccgacttcgt gaccgaggat
    22921 ctcgtcgatt accgcttcgt gaaggcagcg ctcgaccgtg agcccgattg gacgctcgac
    22981 cccagcgtcg acaaagcgag cccgttcgtc aggagcgagg ttgtgaagcc gtgagcgagt
    23041 ccagcttcgt tctgtcggcg cgatcgcctg ccgtagagga tccccccgca tcgggcgaac
    23101 cttcgcgcga gggtgacagg ccgggctcgc tgagagccat gggccagaag atctggccgc
    23161 cagcactcgg catctttggt ctcgccctcg tctggtggct tgcgggcgtc gtgctcgagg
    23221 cctcgccccg gctcgtcgcc ttcaccggct tcgcaccggc accgacgctg gcgagcttct
    23281 ggcagctcct gatgagcggc gatgcatggg aggcggccgc gccgagcctc taccgtgtcc
    23341 tcggcggatt gggttggggg ctggctctcg gggcgcctgt cgggctggcg ctggggctgg
    23401 tgcccttgct ggagagggcg gtgcagctcc cgttccagat cctacgcatg gtgagcccgc
    23461 tgtcgtggat gcccatcgcc atcctcgcgt ttccgacctg ggatggtgcg atcgtgttcc
    23521 tgatcgcggc tgccgccatc tggccgatca tgttcgcgac ggccgcgggc gtgaagcgga
    23581 tcgatccggc ttggctgtcg gtcgggcgca cgctcggcgg ccgtggcttt ccgctcctcg
    23641 gccgcatcgt catgccggca gtactgcctg acctgctgat gggtctgcgg ctcgcgctcg
    23701 gcatcgcctg gatcatcctg gtcccggccg agttcctcgg cgtcacctcc ggccttggct
    23761 acgccatcaa cgatgcccgc gacacgctct cctacgaccg gctcgccgcg ctcgtgctgc
    23821 tgatcgggct gattggcttc gttctcgatc tggctcttgc cgtcgctctg aagcggctga
    23881 gctggacccc tgctgcctga accttaaaca caaaggagac cgatatggct gaaacactcg
    23941 tcaccgagtt catcagcgtc gaccccgagg ggttagccgc gctgtcggcc aagggcaagg
    24001 cggatcccac cgtggtccgc accgtcaagt gccgcaccgt cgccgaaggc cgtcgcttcc
    24061 gccacttgaa ctatatccgt gatctgccgg cgcacatcgt cgatgagccc aaggcgctcc
    24121 tcggcgacga tacggcgccg aacccgacgg aagccctgct ggcggcgctc ggaacctgcc
    24181 tctccgtcgg catccaggcc aacgccgtgg cgcgcggctg gaagatcaac ggcatcaccc
    24241 tcgacctgga aggcgacatc aacatcacgt cggtgtgggg cgctggcgac ctgtcgccga
    24301 agcccgtcgg cctcacggca gtgcgaatcg cggtcgatgt cgacgtggag ggcgcctccg
    24361 aagagcagat agcggagctc gtcgagcatt cggccaaatg gtcaccggtc gtcaacacgg
    24421 tgaagaaccc ggtcgcgatg gagatcacgc cgcgccggct ggacgacgac ccgcgcacga
    24481 ggtaaggccg atgctgaacg ttctcgagcc gcgcggcgag gccgccgcca ttcgcgacat
    24541 cgttgccagc gatatccggc cgaacctgca cgccatcgat agggacgggg tctatcccga
    24601 gaccgcgatg cggcggctcg gcgcggcggg tgcgttcagc catcacattg gtaacagtgc
    24661 aagcgatggc gggctggatc gcgccatcgc cgccatggcg gaggtcggcg agacctgtct
    24721 gtcaacggcc ttctgcatgt ggtgccagga tgcactggcc tggtatctcg accggtccga
    24781 taacgacagc ctgaaacgac gcatgctgcc ggctgtcgcg tccggcaccc ggctcggagg
    24841 caccggcctg tccaacccta tgaaggcgct tgccggcatc gggcgcgtgg cgctcagggg
    24901 agaaagggtc cagggcggct accgggtcac gggccacctg ccctgggttt caaacctggg
    24961 tgacgaccat tcgttcgggg caattttcgc cctggccgac ggccgcctgg tgatggcgct
    25021 catccgctgc gcggccgacg gtgtgacgct ggcgcaaaac gcccatttca tcgcgcttga
    25081 aggcacgcgt acgttctcgg tgctgctgag ggacgtcttc attccggacg gcgacatcat
    25141 tgctgaggat gcgactgcct tcctgccgag gatccgccag ggattcgtcc tgcttcagct
    25201 cggcatggcg ctcggtgctg ctcgcggcgc tgcctcgatc atggagagcg atgcgagtgg
    25261 acgcagcgcc gcggcctggc tgccgcatcc gccggaggcc ctccgggagc gcgccgctgc
    25321 gcttgcgaaa cgggcctcgc agctcgcgcg atcggcggat gatccgagcc gctccgcgtt
    25381 tctcgatgtt ctccgcttgc gcctcgatgg ctcatggctt gcgctgcagg ccacggaagc
    25441 ggcggcgctc cagttcggag cccgcggtta tctcaagggc agcgacgtcg ataggcgtcg
    25501 gcgcgaggcg ctgttcgtgg ccctcatcac gccgtcaatt aaacacatca cgcaggagct
    25561 ggccgcggac tgagggcctt ctcccgtcca ataggggaac acgcgaccaa actagcccac
    25621 cctgcgatgt gggcgcatct gagcctgcgg ggcttgggtg atcgtctttg ggtgttcccg
    25681 tcaattgcgg ctggtacgca ggccgcgcgc cagcggcaga gccgcgagac agagcaggct
    25741 catcagcgcg aacgcggcac cgcccagcca cccgaacagc cagccagagg ccagcatgac
    25801 cagcgccgta gccgcgccga tgccgagcgt gctgtaaatc gcctgggcgg tggcctcgag
    25861 atcttccggc acgttcctgg cgatcagttg catgcaggca aggtggagca gcgcgaaggt
    25921 aatgccgtgc atcggctgga tcaaagacaa gacccagacg tccgtgctga ttgcggcaac
    25981 ggcccagcgc acagctccga cgatggcggc gagcgcgatg gagcccgccg gtgtcaatcg
    26041 ccggatgagc ggcggcccga ccagaaaaaa tacgaccact tccgcgatca ccgccagcga
    26101 ccacagcagg cttgcgacct ggggcgcgat acctgcctcg gtccagcgga tcatggcgaa
    26161 agtgtcgtgc atgccgtggc tgccgaggat caacgccgcc accagcacga tccggcgaaa
    26221 ggcggccgag tgccacaacg tcctcaagcc ctcgttggca acggccgaaa cgctcggctc
    26281 ctcggcattt agcgcgggca ccaatcgcgt caccgccgca gcggcgatga gcagcgctgc
    26341 ttgccaccaa aggatggcgg ccaagccgaa ggaggcaacg gaccagccgg cagcgatcga
    26401 gcccaggatg aaagctgccg agccgctacc gcggacccac ccatactcga agcctctgcg
    26461 cggccggttc gcggccacaa cggcgagcgc gtcggccagt ggcgtgagcg gcgccagcgc
    26521 catggcgtac gctaagttga ccagcagtat gattccgaat gtcgacgcgg gaaggtagag
    26581 caatgtggcc actgcagccg cgacggcgca ggcggcgaaa acaagccgct gtgcttgcca
    26641 atggtccgcg atgcggccgg caagtggtgc cgccacaagg cgcacggcgg ttcccgcagc
    26701 aaagatgaat cctatttgtt cggctggaag accacgcgtc tcgacgaaag ccggcaagta
    26761 gggcgaagcc acgccgaagg atccgtagag gagggaatag aggataacaa agcgcagcaa
    26821 cggcgcagat cgtgtcgtcc tgccctcgct cgccgttcct gccatgaccg atagctcaaa
    26881 aattggagtg tgatttctgg gacttggaac gctccgcgcc cgtctatgga ggtcgagcag
    26941 gggctagatg tcgcccgaca ggccacaagg agcgaactcg cgccagccat aaatggtgcc
    27001 cgaatttcag catcgcttgc gcgaccatca ggaaagccgg cccgaggccg ggctttgtca
    27061 aattttggat tcgcgcgcta tcactgccca ttgccacagg gaaagccgaa cgggtgagga
    27121 tagctgcacg cggaccctgc tgcagcgttg catcgcctca tcatcaccgc ttcgattttt
    27181 cgatcactga ttgtattgaa cttttctggc atgctcattt cgtcagactg ctgctaagct
    27241 acggttgatc caacgtccgt ggggttaccg atgatgcgcg acgggggacg gcggctggga
    27301 agcgcgtggg gcggcaaacg cctcctgatt gcgcttactg ttttctgcgc ccttgaaagc
    27361 ctgctgcccg gaactgcgct ggcagaaggt gcaccgccgc agaactggga aagcgcctat
    27421 caggacgccg cgtacttcta cagcgaaggc gatctgcagc aggccctctc gcatgcgatg
    27481 cgtgctcgac ggatcataga gcagccggga acggacgagc gagatgcact ggcgcaggtc
    27541 ctctcgctcg aggccgttct gctcgaccgc ctaggtgtgc agtcccgaag agagatggaa
    27601 tcagcgtaaa tgattccgca tcacttcggg aggggctatg ggacaggtac ttcacggcag
    27661 cgccaagacc actcacgcca tccgagctgc aatacagcga tcgaaagcta cgatcgcgga
    27721 gctcgccgaa aagtacgacc tcaatcccaa gacggtgatg aaatggcgcc ggcggcagtc
    27781 ggtcgaggac atgccgatgg ggcctcagaa tgcccgctca accgtgctgt cgctggagga
    27841 ggaggccctg tgtgttgcgt tccgcaagca caccctctta gccctggatg actgtctcta
    27901 tgcgctgcag gcgagcatcc cccatctgac ccgatcctcg ctgcatcgcc tgttccagcg
    27961 ccacggcatc tcgcgtctgc caggcatcga aggcgagagg accagaaaga agttcaaagc
    28021 ctacccgatc ggctatttcc acatcgacat cgccgaggtg cgcacggccg aaggcaagct
    28081 gcacatgttc gtggccatcg atcgcgcctc gaagttcgcc ttcgtcgagc tgcacgacag
    28141 ggccacgcga cgcaacgctt cggagttttt gcggcgcttg atcaaggcgg tgccctacaa
    28201 ggtgcacacc gtgctcaccg acaatggcgt tcagttcacc acgcccggta atgtggcctc
    28261 ggcagccaga gacatccgtg acgctctcga tcgcggagag ctcgtctggg cgcatggctt
    28321 cgagctcgtt tgcgcccaga acgatatcga tcatcgcctg accaggccca accacccctg
    28381 gaccaacggc caggtcgagc gaatgaatcg caccctcaag gaggcgacca tcaggcgcta
    28441 ctactacgaa agccacgacc agcttcgcgc tcaccttgcg gccttcgtcg ctgcctacaa
    28501 cttcgccagg cgcctcaaaa cactgaaggg cctgacgccc ttcgagcaca tctgcaaatg
    28561 ctggacagaa gagccgaacc gattcatcgt cgatccaacc cacctctctc cgggactgaa
    28621 cacctaggca ggcacgcgga gtccgcgccg ctgcatcgcc gcgcgctgaa gctgcgcgag
    28681 gagagcctcc atcccgaaga tccccgtctc gcagaaagtc tcaacaatct ggccgttgcc
    28741 gtcgatcttc tcggtcggca ccgcgaggcc gaggctctgc acaagaaggc gctggcaatc
    28801 cgcgagaagg cctttggccc gtttcacccc gacgtcgcgc agagcctgaa caatctggcc
    28861 aaggtacagc ttgccctggg ccggcctgcg gatgcgggac cattgctgcg gcgggcaatc
    28921 tcgatcctct cgagcgggcg aggggagcgc ctgacgcgca gtcaactcga tctggcggac
    28981 ttctccggta cggagcgtga gcccgatgag ggcatcgcac acgtcggcaa ggtgggcgcc
    29041 gcgatgcctg gcccggcacc ggtcgttcgc ccgcgcccgg ctatgcggag tgctcctgcc
    29101 gcaccggact ccgcgacagc cgacgggatg gaccgtgcac cggccgaacc ggccgacgcg
    29161 gctctccatg aagccgatct cgccgtcacc ttgctcaacc tcggcatgat ggcgcagacg
    29221 gcctacaccg ggccgaagca ggaggaagcc ggcctgcttt accggcgggc catcgcgatc
    29281 caggaaaagg tgctcggacc ggagcaccct gatctcgcga cgagcctcaa caatctggcg
    29341 gagctcagcc tcattcaatg gcgctaccgg gaggccgagg cgctgcacaa gcgcgcgctg
    29401 gccattcgcg agaaggcgct cgggccggac catcccctca cggccgccag cctgaccaat
    29461 ctcgcgcggc tctacagcct cacccgtagg ccggagatcg cggccccgtt gctcgttcgt
    29521 gccgtggcga ttacggagaa agccctgggg ccggatcatc cggacacggc gctcgccgtg
    29581 cgtagcctcg ccatgacgca tgcggctctc ggcgatcata ccctagcgct ggactttcat
    29641 cgccgcgcaa gccgatcaat cattgcgcat gcgttgtcct tcgctcaggg agttgacagc
    29701 gacgacagcg acgcggcgca gagcagaggc aggctcaatc atcattttca gagtcacatc
    29761 gcagcgctcc ataggtcagt cgaaactggc gtggaggacg aggctgtcgc gcgcgaagag
    29821 tccttcgagc tggcgcagcg gcttggcaat agcgccgcgg cacgtgccct gacccagatg
    29881 accgcgcgcg tcggacgcgg cccgggacaa ctggcgcagc tcgttcgcga acggcaggac
    29941 ctgaggctgc gctggaagga tgcggatcgg cggctcttgt cggcgctcgg cgccgctgcc
    30001 tctgatcggg acgaaagttt catccgttca cttcgtgagg agcttgccgg tcttgaaaaa
    30061 cggattgcat ccgtcacgga ggtcatcgac agcgcgtttc cagagtacgc gagcctcgcc
    30121 aatccccatc ccgttccgat cggggaggct cagcagctgc tgaacgagga tgaggctctg
    30181 atccaattcc tgatcggcag ctacggcagc tacgtctggg ccgtaaccaa agacaacgtg
    30241 cgctgggtca agctcgctgc gggccggcgc gacatcgtga gaaaggtcga cgcgttgcga
    30301 tgcggtttgg atgccaggag ttggcacgat tacggatcgc aatgccaaag attgctcgag
    30361 accggttaca acttgggcga ttacgagcgc ggtgcgccgc tgccatttga cctcgcgaag
    30421 gcccatgagc tctatcgcgc cttgttcggc ggtatcgagg atctgatcca agataagcat
    30481 cttctggtga tcccctcggg gccgctgacg aagctgccgc tgcatgtgct cgtgacagcc
    30541 tcgccgccaa caagagatcg cggcagcccc gctgattaca gcgccgtcca gtggattgtg
    30601 cgccgccagc cgatgaccat cgtgccgtcg gtgacggctt tgcaggcgct caggtctggc
    30661 gcttcgacga agccaccaac acgtcctttc atcggcttcg gaaatccgct gctctcgggg
    30721 ccggatgggc aggaccgcag ggcctggcaa tttcaaactt gccgtcgcga gccctcgctc
    30781 agcttcgggc aacggctcgc cagcgcgcta caatacgtcc cggcgctctc gtcgctgttc
    30841 cgcggcagct tggccgacgt tcgtgagctg cggctgcaaa tgcctctgcc cgaaaccgcc
    30901 gacgagctgt gcacgatcgc ccgccggctc ggcgctcccg aaccggacgt gtggctcggc
    30961 gaccgcgcga ccgagcaaaa tctgaaacac atcaacgcga ccggagaact ggcacgatac
    31021 ggcgttctgc actttgcaac gcacggtctc gtcgccgggg atctgacagg cttggccgag
    31081 cctgcgctcg tcttgacgcc gcccaacgag gcgagcgatg aggatgacgg actgctcacg
    31141 gcgtccgaag tcgcggaatt ggagcttgcg gcggattggg tagtcctttc ggcttgcaac
    31201 acggccgcgg ggcagggcgg ggacgccgag gcgctctcgg ggttggcacg cgccttcttt
    31261 catgccggcg cccgcgcttt gctggtctct cattgggagg tgaactccct tgcggccgtc
    31321 aagctctcga cggccgcctt cgctgcgctc gccgatcgcc gggcttccgg ccgtgccgaa
    31381 gccatgcgcc aggcgatgct gtccatgatc gagagtggcg aggcgtttga ggcccatcct
    31441 tccaattggg cgccgttcgt cgtcgtgggc gaagggggcg ggggctgagg ccgactgctt
    31501 tgctgcgtgt ctgttgacag cgggcagctt gaaagggagg cgcggtgcag tctccgcgat
    31561 cggctgcagc gccgtgcccg ccgagagcgc ccaagcggac ttggcgccgc aacctgccct
    31621 ctggcgagtt catagccgtg ttctgcgata ttggagaagc agcagcgcac tcatctggag
    31681 cgatgatcgc ggccctgctt cttggcattc tcgaacagct gtctgcggta atttcggttc
    31741 catgtgggcc cgatatatgg aggcggagcg acctttgtcg ggactgtcct tctacgcccg
    31801 ggcggcccgc ccctaacgac cacgccacct tgcgacatcg acgtcttcga gttttgccgc
    31861 gcccagaacg ggccgtggcg gtgatcccgc gttgccgtcg gatggacgat cgttccctgc
    31921 gatctcatgc tgacgcctgc catctgcggg gcagcatggc taccgccggc agaaaggaga
    31981 agcgcgccgg cgacaaggct tgcggcgaga cctgcgaatg gcccacgcgt caaatagatc
    32041 gtcatctttg ctcctcgttt cactgcaaac ttcagatgcc ccgcattgat caacgaggcg
    32101 cgcagcgcgc tcgacgccgc catggctatt tgcgtcggac aaagtcactg gcgccgcgcg
    32161 gcttgaagct ggatgctcaa cgggcgcctt tgcttttcca gctacgggta gcaaaaccgc
    32221 cgacgcccgt cggcttgttg gcggggtggc gatttacttt cctgagccgc tggctgcccg
    32281 ctcgaatagt attcacgccg atcacggaat tgctgcccgc cttcgttcct tttccaccac
    32341 ttggcggaac cgtcttgaac accattttcc agcagccttg ccgccccttg ccgctgtaat
    32401 tgcagctgta atcgatacat ccgcctttga agggaccttt gctgggacga catttggtgc
    32461 acccaaaagc gcctccaccg ctctgcaagt tgtcgccgca gaactctttc accttgtcag
    32521 gcgtggtctt cttgtcggct gccttgacgt cggcagcaaa cagcatgcct cccatcaagg
    32581 tggctaagac caccagatac aaggttcgca cgcgcatttt cgatctcctt tcgtcgcttc
    32641 ctccatagcc gtctgccatg cgtgtttttg tgaaaccccc gaacggtgcc ggggcaatca
    32701 actgccgtgc gatcataggg accggcgatg atcctcgcgc cctccgatcg gaggggaaaa
    32761 aagagagaat cagcggatga ggggattgga aaaactggca acaagcttga cgagatctgc
    32821 ctgacggtgc gtcccggtct tcacgaacag acgccgcagg tggaacctga ccgtcgtttc
    32881 cgccacgccg agagcttcgg ccacctcggg taccccgccg acttcgacgg tggccatgag
    32941 cacgcgcaac tcggtgggcg taagattgta ggttctggcg atgacctctg gtggcgacgg
    33001 gatattcagc gctgccctcc gcacgaacaa cgctgctacc gcggagtaag gagcaccagc
    33061 gccgcggcgc gcccccgaag tcagcggcaa taggtgtgcc acgtacggct cgccgctccg
    33121 cgcggccatt ggcacggcga cgcctttgct cccaatcgct gcatcgccgt tgctggcaga
    33181 gagaaacaca tcgcatagtg cctgctcggc gctcggatcg ccggcaacga tctttccgtc
    33241 aatggctttg agcacggcgc catcggcgag aagatcatgc ccgctggcat tggcatggac
    33301 gatgcgcccg cgcgcgtcga tcaggaagac gccggcactc aagccgtcga ggctatcggc
    33361 gaaagatgcg gcctcggccg ttttgagatc gatcactttg ccgataagga cggcgcggcg
    33421 cacatgcggc acgatgagct gcatccgccg ccgcgtgcct tcgtcggcca ggccggtggc
    33481 ctcgttgcga agaaagctca gatgggcgaa actcgtattt gatttttcaa gaattgcctg
    33541 gaccgagtcg acccagccct gcggccgcgc ccactcctgg aagaagcgcg tttcctgaaa
    33601 ttcctcatac ggcatgaggt cgaccgtgca caccacctct ccgatgtcga agaagaagaa
    33661 cgaggtcgga gtgggatcga gcctggcgta ttggtccagg tagagctgca agtagtgtgg
    33721 atcgacgccg accgagtagg aggcatccgc cgatcggttt accgtgtctc gcgcaatgag
    33781 cccagcggcc tggccgccga cgaaggaggc agtacggcgg agcacgtccg gccacgtcgc
    33841 cggatcgatc accgtgtcgt agatatcacc gatcagagca gaaattttct cgtcttcttt
    33901 cattacgcca tgctccgcgg gcgcagacac attatttcct atatcgatgc tgccaggcgt
    33961 tacaaaaaat cttgtcgctc gctggtgctg ctgattgagt cgcgaccgac gcggttcaag
    34021 ttgaccaaat gatcggatgc ggccggggga ggcgcttttc cgcggagtga tttccgcatc
    34081 cagctccatc gccagtgtgc caattcgtcc tcaaaatcgt gctcatggac ggtcctccca
    34141 atcatgcttc tatttgacaa tacctggagg cccgcgtggg tgaatttatg cggaggtttc
    34201 gcgaccaaag tcactaaagg acttccgccg cgtataatgg gagcctaaac ctcttgattg
    34261 gcccaatcca agacacgtag agcaggtccg cgcggcgaag cccttacctg gaggcttcgt
    34321 tgccacctgc catctcaccg ggcatcatcg aggcgatcgt gattcgcttt gcgcgggcgg
    34381 ccggccaccg agtgcgaaca tcggcagatc actgccaaaa gagcagatca ctgccgtaag
    34441 aaaagaactc gatattgctc agcgacagca gctcccttcg ccgccgcccc gctcaagctg
    34501 gatcgccggg caggccaccg aaccgtagga gcagaatacg cagcagccgc ccggttccgg
    34561 gcgcaccatt gtgtgacagc actcgcactt cgtagaagaa ttgacaggcg tcggtcggca
    34621 ttgtctcgac cttgcgatgg ccgccgtgcg ggcaggtgat ctcggagcga agctcggctg
    34681 gatggtcatg gcgacaccgc tagcagcttg ggcaggcgcg ccagattgga tgcggccgcc
    34741 gtgaaggcca aggcccgctt gcgggcaagg acggagcggc aacttattga tttggtgagc
    34801 ccgtcgggat tcgaacccga gaccctctga ttaaaagcaa tcagcctacg ttacgatgtc
    34861 tcaacatttt cagtcacaga aagcgagcat ctgatcgaaa atcagaagct caaagccgaa
    34921 aagcgaagaa gtatcaacca tttacgtatg gcatcacatg caactccggc cttggtaccc
    34981 ccttggaacc caaaccgtat ccacaccatc agcttgcacc atttttgacg gttgctattc
    35041 tgttctcata caagctagtc cgttcaggtc tgcccgtcgt gactagcacg ggcagggttg
    35101 agtgtaatta caatgaagca gtatttttac gataacgccg agcagaacct atacgtgttt
    35161 gacggcgaaa ccgaagaatt gaaaatcttg ccacgactac gtgtgcgcgt agtaataggt
    35221 cttaccgatg cagaagatga ccgtgcgcga tctgggattc caaccggagg agctgcacgt
    35281 gtccgcaaca acatgaaacg aacaataacc tgcaaaaact gcgggcagac agggcatcaa
    35341 atgcgtaaat gtccgaatcc gataaacgga ccgcttgagt cggatgctga ttactaaccg
    35401 aaataataac cataaagccg atctgcgata tgaaaaaagg tgccaagaat tagaaacatc
    35461 aacattaaac cgataatcca tcggataaag cgctgaccgg cccgatagaa cgtcctgcta
    35521 tttctcctgt accgcactct gccctcacag caaaagcccc gccgaagcgg ggctttgctt
    35581 cctcacaaac tgactgcgcc cgtatctgtc actactactc ccgtgcttgg tggcactgat
    35641 cggtagtgct gatggggaag atccggcaag tgggccggtg gctgctcaca ccaatggcca
    35701 ggcaagcact gatggccctt gtggctgccg ctccacaacg ccatcccaca caccatgcac
    35761 acgatacctt ctttccattt gatcggcatt tcgcgtctcc atcatcagtc aaacctgacg
    35821 cataccgacg caaagaacat ttcgcgaaca tcaacttgtg gactgggacc cccgcggaac
    35881 cccagaaacc tctggaaccc tatctaactc gataaccctt tgattttatt ggtgagcccg
    35941 gttgggatcg aaccaacgac cctctgatta aaagaattag cgtcttacaa aaaacccttt
    36001 cgtttcaatg catttggctt cagctcgtgc cggttcgttc cgtctctgtg caggggtttc
    36061 atgggcgttt catgggcggt tgcggcggcg gccgtcggca cgcgcggcat cgctctggaa
    36121 gtcggggtga tggtggccgt agacctcctc caaggtcttc tcgctcatcc cgagatagcc
    36181 ggcggcctcg cgcttatcga cgccggcctg catgagccac gtcgcagcag tatggcggca
    36241 tacgtggggg ccatcgggta tctgccagat gggattgcca tcggcgtccg tgccgatctg
    36301 cttggcatgg cccgcggcga tcgccaccga cgcccaggag cggcgcagct tctcgatggg
    36361 cttgccgtaa tagtggatca cgtaggtcgt tttgaccgtc cgttcgacgc gccggccacg
    36421 ctcgctgata cggcgcttga tgccgatctc catgtcggcc cggtaccagc ggcggagcca
    36481 cggcagcaag cggcggtgga tgcgcgcctt cggcttgcgc ttgttgttct ccgcctcgcc
    36541 ctgggcgcgc cggtgcagcg tctccgtctc gagatcgaac cagccgccgg aggtcgaagg
    36601 gatccagcgg agcttgcgcg cggcgccggg ccgtgtgcct gtgtagacgc cgataagcag
    36661 cgcacgaacg atgtgcttgc actgcggcat cctgcgggca acccgtagcc gctcggcgac
    36721 ctctttgcgc gtcagccaat agtcctcgcg cggcggcgtc gccggcggca gcttcatcgc
    36781 cgggattgac gtcaacggcc
//
LOCUS       DASVDU010000110         4927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1585974, whole genome shotgun sequence.
ACCESSION   DASVDU010000110 DASVDU010000000
VERSION     DASVDU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585974"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="VF226_07110"
     CDS             <1..133
                     /locus_tag="VF226_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7073795.1"
                     /translation="DRLTVACGEGAVRLTLLQRAGRKPMSAEEFLRGFDLPAGTKLG"
     gene            530..1327
                     /gene="truA"
                     /locus_tag="VF226_07115"
     CDS             530..1327
                     /gene="truA"
                     /locus_tag="VF226_07115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX7073796.1"
                     /translation="MPRYRITIEYDGTPFVGWQIQANGISVQGELVRAIAQFSGEETS
                     VRGAGRTDAGVHALGQVAHFDLARVWEPFKIREALNYHLRPHPIAVLDCELAPDGFDA
                     RFSARTRHYLYRILSRRAPPALDRNRVWWQPMPLDAAAMHEAAQVLVGRHDFSTFRAA
                     ACQAKSPIKTLDRIDVSAAGEEIHIRTSARSFMHHQVRSIVGSLKLVGAGRWTRTDLA
                     HALQARDRAACGPVAPAHGLYFVGVDYPASDEAAMAPTEEADEDAEA"
     gene            complement(1261..2676)
                     /locus_tag="VF226_07120"
     CDS             complement(1261..2676)
                     /locus_tag="VF226_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase family protein"
                     /protein_id="HEX7073797.1"
                     /translation="MPSTVFDSSVFKDAFGTPEMRNVFSDETAVALAVKVEVALARVQ
                     GRLGIIPAEAAEAIAQRADASAVHLGELKRETELVGYPIVGLVHQLARQCGDAGRYLH
                     WGATTQDIMDTVSVLQIREALRLIATDLARTDEALAGLAERHRLTVMAARTHLQHALP
                     TTFGHKAAVWLSMIRRDRERLRQLWRRVIVLQFSGAAGTLASLGNRGLEVQEALAAEL
                     GLAAPTITWHAARDGFAEVVNFLALTTATLAKIATDVALLMQTEVGEAFEPFVPGRGS
                     SSTMPQKRNPISCELIIAAAKAVRQFAGLMLDAVVSDHERATGPWQAEWIALPQSFIA
                     TAGALRRSVELLSGLVIDENRMKRNLELTGGLIVAEAVMMALAEYIGRGAAHDIVYSA
                     CRSAADSGNELITELRRRPEVTAHLDDAALQKLVDPANYLGSAPAMIDRALSERGGAD
                     QASASSSASSVGAIAASSEAG"
     gene            3081..3593
                     /locus_tag="VF226_07125"
     CDS             3081..3593
                     /locus_tag="VF226_07125"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073798.1"
                     /translation="MAVEGDDRERAAGVFRLLNSTAEKLALFGGLLTVAAALLVCASV
                     GLRWITSNSIPGDFELVQIAVALSAFAYLPFCQLRRGNIAVATFTSTLPAWVQASLDA
                     LWDIVYAATAAFIAWRLAAGAAETIANQTTTMVSGIPLGWAIAATSAMAVLLALAAAA
                     TAVQLIRRRS"
     gene            3590..4900
                     /locus_tag="VF226_07130"
     CDS             3590..4900
                     /locus_tag="VF226_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7073799.1"
                     /translation="MSGTTTAVIGFAAMLVLIGLRMPVGLAMLLTGSVGYVSFMGVAP
                     FLSYMKTTPYHLFANYTLSVIPLFILMGAFAERSGLSTDLFSAAARLFGRRKGGLSIA
                     VIGASAAFGAICGSSVATTATFSRAALPELKRYGYDPGFASATVAVGGTLGILIPPSV
                     ILVVYAIATEQNIVKMFQAALIPGLMAACFYCLVANWVARHDPAAGPAVDPDAVGGSW
                     RGVWPALVVAVVVVGGIYGGVFTPTEGAAVGAAAMLLIGMARRTLGLAEFRVSLLQTA
                     ETTGMIFLILLGAEVFNSFLALSRLPTEMAEAIAASGYPPLAVLAILLAAYIVLGSVM
                     DELAMILLTLPVFFPVVSALDFGLPAEEVAIWFGILVLIVVGVGLVAPPIGLNVFVVS
                     ALAKDVSIISIYRRVLPFLAMDIVRLVLVTTFPALALVLVRLLN"
BASE COUNT          749 a         1634 c         1635 g          909 t
ORIGIN      
        1 cgatcgcctc acggttgcct gtggggaggg cgcggttcgt ctcacgctcc tgcaacgtgc
       61 cggccgcaag ccgatgtcgg cagaggagtt cctacgcggc ttcgacctgc ctgccggcac
      121 gaagctcggc tgagctgctt ctgacgtttc tgccccacag cgtcccgggc cttgtgcccg
      181 ggatccaact ctcggcaggc acgtgggctg gtggctggca tcagggatca gcaagcaagc
      241 ggggcagcgg cacccgccgc ctcgggaggt acgtctccgg cggaaccggg gcgagccgtt
      301 acgcgatgca cagagttcga tgagagatgg atcccggcga tgaacgccgg gatgacctgt
      361 gtttttggcc gcttgctgaa gtgtcatccc gggccttgtg cccgggatcc aaccctccgc
      421 cggtacccgg tcggcggctg gcacccccag caaaccaacg aagcgtcggt agccgccgcg
      481 tcgcggtgcg tcttgccgga gcaggggtga gccgttacca gtggccggca tgccccgtta
      541 tcgcatcacc atcgaatatg acggcacgcc gttcgtcggc tggcagatcc aggcgaacgg
      601 catctccgtg caaggtgaac tcgtccgcgc catcgcgcag ttctcgggcg aggagacaag
      661 tgtgcgcgga gcagggcgga cggatgccgg tgtccatgcg ttgggccagg tggcccattt
      721 cgacctcgcc cgcgtctggg agcccttcaa aatccgcgag gcactgaatt accacctcag
      781 gccgcacccg attgcggtgc tcgattgcga gctcgctccg gacggcttcg acgcccgttt
      841 ttccgcaaga acgcgccatt acctctatcg aattctgagc cggcgcgccc ctccggcgct
      901 cgatcgcaac cgcgtgtggt ggcagccgat gcccctcgat gccgctgcca tgcacgaggc
      961 ggcgcaagtg ctcgtcggcc ggcatgactt ctcgacgttt cgcgcggccg cctgccaggc
     1021 gaagtcgccg atcaagacgc tggatcgaat cgatgtctct gcggcaggcg aggaaatcca
     1081 catcagaacg tcggcccggt cgttcatgca tcatcaggtg cggtcgatcg tcggcagcct
     1141 gaagctcgtc ggcgccgggc ggtggacacg gaccgacctc gcccacgcgc tccaggcgcg
     1201 ggacagggca gcgtgtgggc cggtggcccc tgctcatggg ctctatttcg tcggcgtcga
     1261 ttatccggct tccgacgagg ccgcaatggc gccaaccgaa gaagcagacg aggatgcgga
     1321 agcctgatcg gcgccaccac gttcgctgag ggcgcggtcg atcatggctg gtgcactgcc
     1381 gagatagttg gccgggtcca cgagcttctg cagcgcggcg tcatcgagat gggcggtgac
     1441 ctccggacgg cgacgaagct ccgtgatcag ctcgttgccg gagtcggccg cggaacggca
     1501 tgcggaatag acgatgtcgt gggcagcgcc gcggccgatg tactcggcga gcgccatcat
     1561 caccgcctcc gcgacgatga gccctccggt cagctcgagg ttgcgcttca tgcggttctc
     1621 atcgattaca agtccgctga gcaactcgac agaccggcgg agcgccccgg cggttgcaat
     1681 gaagctttgg ggcagggcga tccattctgc ttgccagggg ccggtggccc gctcgtggtc
     1741 ggaaacaacg gcatcgagca tcagcccggc aaactggcga accgccttgg cggccgcgat
     1801 gatcaattca catgagatcg ggttgcgctt ctgcggcatg gtgctcgacg agccgcgccc
     1861 aggcacgaac ggctcgaacg cctcgccgac ctccgtctgc atgaggagcg cgacatccgt
     1921 cgcgatcttg gcgagtgtcg ccgtcgtgag cgcgaggaag ttgaccacct ctgcgaaccc
     1981 gtcccgcgcc gcgtgccagg tgatggtggg agccgccagg ccgagctctg ccgccagcgc
     2041 ttcctgcacc tcgagcccgc gattgccgag cgaggcaagc gtcccagccg caccggagaa
     2101 ctgcagaacg atgacgcgac gccagagctg gcgcagccgc tcccggtcgc gccggatcat
     2161 cgacagccag acggcagcct tgtggccgaa ggtcgtcggc aatgcgtgct gcagatgggt
     2221 gcgggcggcc atgaccgtca gccgatgtcg ctcggcaagt ccggccagcg cttcgtctgt
     2281 gcgggcgagg tccgttgcga tgaggcgaag cgcctcgcgg atctgcagca cggacacggt
     2341 gtccatgatg tcctgggtcg tcgcgcccca atgcagatag cggcccgcat caccgcattg
     2401 gcgcgcgagc tgatgcacga ggccgacgat cggatagccg acgagctccg tttcccgttt
     2461 gagctcgccg aggtggaccg ctgatgcgtc ggcccgctgc gcgatcgctt cagccgcttc
     2521 ggccggaatg atgccgagcc ggccctgcac gcgcgcaagg gccacctcca ctttcacggc
     2581 aagcgcaact gcggtctcat cggagaagac gttgcgcatt tcgggcgtgc cgaatgcgtc
     2641 cttgaagacg ctggagtcga aaaccgtgga tggcatgcga tcgcctcgcg ctggtgtccg
     2701 tcgccctagc gtccacgcca tcggcattgc aggcaagagg gcgctcgatg ctcgcgtcac
     2761 aaaaacgaaa aaaggcccgc cgggatgcga cgggccgagt tttcggggag gaaacggacc
     2821 cacgcggcaa ccggaggtgt gtcgcacact ccgatgagcg aggtgagtcg accgccgaac
     2881 gcacgacgcc gcgtgaagat ctcgggtgcg gcaatatgag tggacttcga gcctcggggt
     2941 cccgagcgcc cggctgatcg ccgacgtcca ttgtgtagcg agctagcctc gccttaagct
     3001 ttccggtatc tcgggagctt gggcactccg tgggttggcg ggtgtccacg cgcagactgg
     3061 agcggcacca ggaggcatgc ttggcagtcg agggtgatga ccgtgagcgc gcagcaggtg
     3121 tcttccgcct gctcaattcg acggctgaga agctggcgct gttcggcggc ctcctcaccg
     3181 tagccgcggc tcttctggtg tgtgccagcg tcggcctgcg ctggatcacc agcaattcaa
     3241 tcccaggcga tttcgagctg gtccagatcg ccgtggcgct ttcggccttc gcttaccttc
     3301 ccttctgcca gcttcgtcgc ggaaatatcg ccgtcgcgac cttcacgagc acgctgccgg
     3361 cgtgggtaca ggccagcctc gatgcgctct gggacatcgt ctatgccgcg acggcagcgt
     3421 tcatcgcctg gcggctagcg gcgggagccg ccgagaccat cgccaaccag acgaccacga
     3481 tggtttcggg aattccgctc ggctgggcca tcgccgcgac atctgccatg gcagtgctgc
     3541 tcgccctcgc tgctgctgca accgccgtgc agctcatcag gagacgatcg tgagcggcac
     3601 gaccacggcc gtcatcggct ttgccgcaat gctggtgctg atcgggctga ggatgccggt
     3661 gggcctcgcc atgctgctca cgggcagcgt cggctatgtc tccttcatgg gcgtggcgcc
     3721 gtttctgagc tacatgaaga ccacccccta tcacctcttc gccaactaca cgctctcggt
     3781 cattccgctc ttcatcttga tgggggcgtt cgccgagcga tccgggcttt cgacggacct
     3841 cttcagcgct gccgcgcgcc tgttcggacg gcgcaagggc ggcctctcca tcgccgtcat
     3901 cggcgcttct gccgcgttcg gcgccatctg cggctcgtcc gttgccacca ccgcaacgtt
     3961 ctcccgggca gctctcccgg agctgaagcg ctacggttac gatcccggct tcgccagcgc
     4021 caccgtggct gtcggcggca cgctcggcat cctcattccg ccgtcggtca ttctcgtcgt
     4081 ctacgccatc gccacggagc agaacatcgt gaagatgttc caggcggccc tgatcccagg
     4141 actcatggcc gcctgcttct actgtctcgt cgcgaactgg gtggcgcggc acgatcccgc
     4201 cgcgggcccg gcggtcgacc cggatgccgt aggcggctcc tggcgcgggg tctggccggc
     4261 attggtggtg gccgtcgtgg tcgtaggcgg gatctatggc ggcgtcttca cgccgacgga
     4321 gggcgcagcc gtcggcgctg ccgccatgct gctcatcggc atggcccggc gcacgctcgg
     4381 cctcgccgag tttcgcgtca gcctgctgca gacggccgag acgaccggca tgatcttcct
     4441 gattttgctc ggcgcggagg tgttcaacag cttccttgcg ctttcacggc tgcccaccga
     4501 aatggccgag gccatagcgg cgtcgggcta tccgcccctg gctgtcctcg ccatcctgct
     4561 cgctgcctac atcgtgttgg gctcggtgat ggacgagctc gccatgatcc tgctcaccct
     4621 tccggtgttc ttcccggtgg tgtcggcgct cgatttcggg ctgcccgcag aagaggtggc
     4681 gatctggttc ggcatcctcg tgctgatcgt ggtgggcgtc ggactggtcg cgccgccgat
     4741 cggcctcaac gtgttcgtgg tgagcgcgct ggcgaaggac gtctccatca tatcgattta
     4801 ccggcgggtg ctgccctttc tcgctatgga catcgtgcgc ctcgtcctgg tcacgacatt
     4861 ccccgcgctc gcactcgtcc tcgtgcgtct gctcaactga gcggactggc ttgtccgcgt
     4921 gggtgac
//
LOCUS       DASVDU010000111         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1199199, whole genome shotgun sequence.
ACCESSION   DASVDU010000111 DASVDU010000000
VERSION     DASVDU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199199"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2347
                     /locus_tag="VF226_07135"
     CDS             <1..2347
                     /locus_tag="VF226_07135"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HEX7073800.1"
                     /translation="PDLRKQLGVWYTPSEVVTYMVARVDKALRDDLGVADGLAAENVY
                     VLDPCCGTGAYVTAVLKRIDRTLDEKGLGGLKGQMVKKAALERVLGFEILPAPFIVAH
                     LRVGSELQRLGASLKDDERASVYLTNALTGWEPENKKPLPFPGLEEERKKAETLKQEA
                     PILVILGNPPYNGFAGVSIGEERDLSEAYRKTERVRRPEGQGLNDLYVRFFRMAERRI
                     VEKTGHGIVCFISNYSWLDGLSFTGMRERYLEVFDAIRIDCLNGDKYKTGKTTPDGKP
                     DPSIFSTEHNREGIQVGTAIATLVRKAEHTPVTTVDFRHLWGTSKWQDLLATAEADPT
                     EVYESLQPALELGLPLMDAAAEVSYFSWPMLPELLPVSVPGVKTSRDDFLISIDREVL
                     EQRIDEYFDPEISDDEIRARYPSVMAASGRFNPTDTRRELLKRGRLPNIVRYAYRPFD
                     IRWLYWESETKLLDEKRSEFRPLIFQSNIFLEAREKQPKAEFSRGTITSCLSDNFGNG
                     LSTFFPVRLSAGKANLPQSIGEFLVNRGLSLDSVFHHIVATLHSPLYRTENAGALRMD
                     WPRVPLPGDPDTLRASAALGATLATLLDPETPAPGVSIGTLRVGLKTMGLPTRRGGGS
                     LDGDDLKLMARWGSIQSNNGSRIVMPGLGLVAERAYTGEERAALAQEGDTVGLALDEV
                     FALIGDKTYDVHLNADAWWSNVPARVWEYTLGGYQVIKKWLSYREHAVLGRALKPEEV
                     AYVSEMVRRIAAILLMGPRLDDNYAASKAAAVEWKDGAPVV"
     gene            2440..2721
                     /locus_tag="VF226_07140"
     CDS             2440..2721
                     /locus_tag="VF226_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073801.1"
                     /translation="MARDQTLDTFGGSQLELFGGAPAPASRATADEMREYLHRLLAEA
                     RAAETMPWPPLTAKLYRGLFPQLSFWLPEDESAQLRLEFETELARLEAA"
     gene            complement(2761..3540)
                     /gene="surE"
                     /locus_tag="VF226_07145"
     CDS             complement(2761..3540)
                     /gene="surE"
                     /locus_tag="VF226_07145"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX7073802.1"
                     /translation="MRILLTNDDGINAPGLEALRDVARELSEDIWTVAPEIDQSGASH
                     SLTLREPIRMRKLDERTFAVRGTPADCVIMGIRYLLRDDLPTLVLSGVNRGSNIADDV
                     AYSGTIAGAHEGTLLGVRSIAMSLACDPRNKIRWDTVRAHGPEIVRRLLAADWPQGVL
                     MNVNFPDCAPEEVVGIAVTEQGRRDHDLVRIDQRLDTWGNPYYWLGYERKRSNPRGGT
                     DLWAIYTGRVSITPLSINHTARNVGETLSRQLERGTEGEGR"
     gene            complement(3591..>4019)
                     /locus_tag="VF226_07150"
     CDS             complement(3591..>4019)
                     /locus_tag="VF226_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX7073803.1"
                     /translation="ELVSITTPEQSLAEHERMTACAEEVLKRLGLAYRTMLLSTGDMG
                     FASQKTYDIEVWLPGQNTYREISSCSVCGDFQARRMNARYRPKDGKPEFVHTLNGSGL
                     AVGRTLIAVLENYQQADGSVMIPKALRPYMRGQERITRTD"
BASE COUNT          746 a         1256 c         1249 g          768 t
ORIGIN      
        1 cccggacctg cgcaagcagc tcggcgtctg gtacacgccg agcgaggtcg tcacctacat
       61 ggtggcgcgc gtcgacaagg ctttgaggga tgatctcggc gtcgcggacg gtttggcggc
      121 cgagaacgtg tacgtgctcg atccctgctg cggcaccggc gcttacgtga cagccgttct
      181 caagcgcatc gaccgcacgc tcgatgagaa gggattgggc ggcctcaaag gacagatggt
      241 gaagaaggcc gcgcttgagc gtgtgctggg tttcgagatc ctgccggctc cgttcatcgt
      301 cgcgcacctg cgggttggca gcgagttgca gcggctcggt gcaagtctca aggatgacga
      361 gcgcgccagc gtctatctca cgaatgcctt gaccggctgg gagcccgaaa acaagaagcc
      421 gctgccgttt cccggtctgg aggaagagcg caagaaggcc gagacattga agcaggaggc
      481 gccgatcctc gtcattctcg gcaatcctcc atacaacggc tttgccggcg tctcgatcgg
      541 cgaggagcgc gacctgtcgg aggcctaccg caagaccgag cgagtgcgaa ggccagaggg
      601 ccaggggctc aacgatcttt acgtgcgctt cttccgtatg gcggagcggc gcatcgtcga
      661 aaagaccgga cacggcatcg tttgctttat ttcgaattat tcctggcttg atggcctgtc
      721 gttcacgggc atgcgggagc gctatctcga agttttcgat gccatccgca tcgactgcct
      781 caacggagac aaatacaaga ccggcaaaac cacgccagac ggtaaacccg acccttccat
      841 cttttcgacc gagcacaatc gcgagggcat ccaggtcggc accgcaattg ctactctcgt
      901 gcgcaaggcc gaacatacac ccgttaccac ggtcgatttt cgtcacctat ggggaaccag
      961 caaatggcag gatttgctgg caactgcgga agctgatccg accgaggtct atgaatcttt
     1021 gcagcctgca ctcgaattag gattgccttt gatggatgca gcagctgagg tgagctattt
     1081 cagctggccc atgctgccgg agctgctgcc agtgtcggtt ccaggcgtga agacaagccg
     1141 cgatgatttc ctcatcagca tcgatcgcga agttctagag caacggatcg atgagtactt
     1201 cgatcctgaa atttcggacg atgagatacg tgccaggtat ccatcggtta tggccgcttc
     1261 cggacgtttc aatcctaccg atacgcgccg cgaattgctg aagcgcggtc gtttaccgaa
     1321 tatcgtgcga tacgcttatc gaccattcga catcagatgg ctatattggg aatccgaaac
     1381 caaattgctc gatgaaaagc gctccgaatt tcggccgctt atattccaat ctaacatctt
     1441 ccttgaggca cgcgaaaagc agccaaaggc tgagttcagc cgcgggacta ttacaagctg
     1501 tctttcagac aacttcggta acgggctctc gacctttttt ccagtgcgcc tgagcgccgg
     1561 caaagctaat cttcctcaat ctatagggga gtttctggtc aaccgaggac tatccctcga
     1621 ctcagtgttc caccacatcg tcgcgacgct ccattcgccc ttataccgaa cagaaaacgc
     1681 tggcgcgctc cgtatggact ggccccgcgt gcctctcccc ggcgatccgg acaccttacg
     1741 cgcttccgcg gcactcggag caacgctcgc cacgctgctc gatccagaaa cgcctgcacc
     1801 tggcgtctca atcggcacgc tccgcgttgg tctgaaaacc atgggtctgc caacccgacg
     1861 cggtggcgga agtctcgacg gcgatgatct caagctgatg gcccgctggg gttccatcca
     1921 aagcaataat ggcagccgca tcgtcatgcc cggtctgggc ctggtggccg agcgggccta
     1981 taccggcgaa gaacgggcgg cacttgccca agagggagat acggtcgggc tcgcgttgga
     2041 cgaggttttc gctctcatcg gcgataagac ctacgacgtt catctcaacg ccgacgcgtg
     2101 gtggtcgaac gtgcccgcac gcgtctggga gtacacgctc ggcggctacc aggtgatcaa
     2161 aaagtggctc tcctaccggg agcacgccgt gctcggccgc gccttgaaac ccgaggaggt
     2221 ggcctacgtc tccgagatgg tgcgccgcat cgccgccatc ctactgatgg ggccgaggct
     2281 cgacgacaac tacgcagcct caaaagccgc ggccgtcgag tggaaagacg gcgcgccggt
     2341 ggtgtgatca tcatgcctgg gggaaagctc ggtcgcctct ggcgtcactt ggacgaatca
     2401 ccgaaccttg ccacgtttgc aggcctgctt ggggaaacaa tggcgcgcga ccaaacgcta
     2461 gacaccttcg gcggcagcca gctcgagctt ttcggtggcg cgccagcgcc ggcctctcgc
     2521 gcgactgcgg atgaaatgcg tgagtacctg caccgcttgc tggcagaggc gcgtgcggcc
     2581 gagacgatgc cttggccgcc actgacggcc aagctctacc gcgggctctt ccctcagttg
     2641 tcgttctggc tcccggagga cgagagcgct caactgcgct tggagtttga gacggagctg
     2701 gcgcgtttgg aagcggccta agccgcgtcg tggacctgag ctgaaaagct cgaatttcga
     2761 ctaccgcccc tccccctccg tccctcgctc aagctgccgc gacagcgtct cgcccacatt
     2821 ccgcgccgtg tgattgatcg acagcggcgt gatggacacg cggcccgtgt agatcgccca
     2881 caggtcggtg ccgccgcggg ggttggagcg tttgcgctcg tagccgagcc agtaataggg
     2941 gttgccccag gtgtcgagcc gctggtcgat gcgcaccagg tcgtggtcgc gccggccctg
     3001 ctcggtcacc gcgatgccga caacctcctc cggcgcacag tcggggaagt tgacgttcat
     3061 cagcacgccc tgcggccagt cggcggcgag cagacgacgg acgatctcgg gcccgtgcgc
     3121 gcggaccgta tcccatcgga tcttgttgcg cggatcgcag gcaagactca tggcgatcga
     3181 gcgcacgccg agcagcgtgc cttcatgcgc gccggcgatg gtgccggaat aggcgacgtc
     3241 gtcggcgatg ttcgagccgc ggttgacgcc cgacagcacc agcgtcggca ggtcgtctct
     3301 cagcaaatag cggatgccca tgatcacgca gtcggccggc gtgccgcgca cggcgaaggt
     3361 gcgctcgtcg agcttgcgca tgcggatggg ctcgcgcagc gtcagcgagt gcgaggcgcc
     3421 gctctggtcg atttccgggg cgaccgtcca gatgtcctcc gacagctcgc gcgccacgtc
     3481 ccgcagggcc tcgagccccg gcgcgttgat accgtcgtcg ttggtcagca ggatacgcat
     3541 tccgcccgca cttcctgttc atccctgtcg caaggagaca gggctcatcc tcagtccgtc
     3601 ctcgtgatcc gctcttgccc tcgcatatag ggccgcaagg ccttagggat catgacgctg
     3661 ccgtcggcct gctgatagtt ctccagaacc gcgatcagcg tgcggccgac ggcgaggccg
     3721 gagccgttga gcgtgtgcac gaactccggc ttgccgtcct tcggccggta gcgcgcgttc
     3781 atgcgccggg cttggaaatc gccgcacacg gagcagctcg agatctcgcg gtaggtgttc
     3841 tgccccggca gccagacctc gatgtcatag gtcttctggg aggcgaagcc catgtcgccg
     3901 gtcgaaagca gcatcgtgcg gtaggcgagc ccgagccgct tcagcacctc ctcggcgcag
     3961 gccgtcatgc gctcgtgctc ggcgaggctt tgctccggcg tcgtgatgga gacgagctc
//
LOCUS       DASVDU010000112        13991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_418194, whole genome shotgun sequence.
ACCESSION   DASVDU010000112 DASVDU010000000
VERSION     DASVDU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13991
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418194"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1028
                     /gene="mtgA"
                     /locus_tag="VF226_07155"
     CDS             219..1028
                     /gene="mtgA"
                     /locus_tag="VF226_07155"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX7073804.1"
                     /translation="MALQDKVREVLAGSPSRGANEESWAARLASHAPSCARVALIGGS
                     LAIAGALGAFIVLSSVLLLFFRFVDPPGSTLMLGQALTGGNVEQRWVDLEDISPNLVR
                     AVISSEDGQFCTHYGVDLGELAAALRKAERSGLDDARGASTITMQVAKNLFLWPSRDL
                     LRKGLEIGMALIMEQIWPKRRILEVYLNIVEWGPGIFGAEAAARHHFKKPASRLTARE
                     AALLAASLPNPHKRIASRPGPHTRRISYIVQARARSAGQRTSCVLPVEGRT"
     gene            1180..1365
                     /gene="rpmF"
                     /locus_tag="VF226_07160"
     CDS             1180..1365
                     /gene="rpmF"
                     /locus_tag="VF226_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7073805.1"
                     /translation="MAVPRKKTSPAKRGSRRSADALKAPTYIEDKESGERRRPHHIDL
                     KTGKYRGRQILEAKDQV"
     gene            1483..1896
                     /locus_tag="VF226_07165"
     CDS             1483..1896
                     /locus_tag="VF226_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073806.1"
                     /translation="MSLRAIPLIAVALILYNAIVLLGGSETPSEILSTRLFEIPMLHE
                     NAVWIFTWGDFLILLVLVLLFIELLKATYTTTASLLDHGLSMIVFVVCLIEFLLVRQA
                     ASSVFFFITMAALIDVVAGYTIGIRVARRDFQIGE"
     gene            1991..3376
                     /locus_tag="VF226_07170"
     CDS             1991..3376
                     /locus_tag="VF226_07170"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEX7073807.1"
                     /translation="MTRLIKLLPVPAAGAGEQSAERDVPLPARPDDVSAGCGEDPHAL
                     AQLERDELTCLVARRHLVEALDHILAGDGQEPWAFLLIGINDLPVTNEMFGFEVGDEL
                     IAAVAHVLTAELEEGWLLGRYSSNKFGLLVPAASAEEMRKTAERLSRAVREARLETSA
                     CRLATTISIGGLLLPQQAASSSEALSRAQGALSRSRYGSFGGFAAYDPGWAHDYVRGR
                     NVRIARDVVSALESGRMRLALQPIVSTATWQPVFHECLLRMELPEGRTIAAGKFIGVA
                     EQLGLARLVDRRALELAIDLLHDRPDLHLALNVSGLTCTDHDWLVALQGLTSAGGLAE
                     RLTIEITETAAIQDFDHSVTFVDALKELGCRVAIDDFGAGYTSFRTLKHLAIDMVKID
                     GAFVRTLPRDSTDQVFIKAMVELARSLNLSTVAEWVGDEATARLLADIGVDYLQGFHF
                     GEPVVFGANSE"
     gene            complement(3425..4093)
                     /gene="phaR"
                     /locus_tag="VF226_07175"
     CDS             complement(3425..4093)
                     /gene="phaR"
                     /locus_tag="VF226_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX7073808.1"
                     /translation="MLSKSDPPADKKEAVVIKKYANRRLYNTETSTYVTLEDLAAMVR
                     SDRDFVVYDAKTGDDLTHSVLTQIIVEQEGRSGGQTLLPIPFLRQLIRFYDDSIARMV
                     PSYLQMSLETLAKEQERFRAQFSHMLGQSPFGAAALDAMQEQTRKNMAMFEKAMAMWT
                     PFGTQAPGPKPQEEADQAGAAKGAEPAQEKAGSEEATKSELEDLKAQLASMQEKIEKL
                     SQKR"
     gene            4337..5524
                     /locus_tag="VF226_07180"
     CDS             4337..5524
                     /locus_tag="VF226_07180"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7073809.1"
                     /translation="MSADNSTIVIASAARTPVGSFNGSLSSLPAHELGKVAIKAAIER
                     AGIEPGEVDEVIMGQILTAGAGQNPARQASIAAGIPVDSPAWGMNQLCGSGLRAVALG
                     AQQIQLGSSSIVVAGGQESMSQAPHCAHLREGTKMGDLKLIDTMIKDGLWDAFNGYHM
                     GSTAENVARQWQITREEQDRFAVASQNKAEAAKKAGKFKDEIAPVTVKTRKGETVVSE
                     DEYIREGTTYEAVAKLRPAFEKDGTVTAGNASGINDGAAAVVLMTLAEAERRGITPLA
                     RIVSWAHAGVDPSIMGTGPIPASKKALEKAGWSVADLDLIEANEAFAAQACAVNKGLG
                     WDTEKVNVNGGAIALGHPIGASGARILNTLLFEMKRRDAKKGLATLCIGGGMGVAMCV
                     ERV"
     gene            5658..6383
                     /gene="phbB"
                     /locus_tag="VF226_07185"
     CDS             5658..6383
                     /gene="phbB"
                     /locus_tag="VF226_07185"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX7073810.1"
                     /translation="MARVAVVTGGTRGIGAAISIALKNKGYRVAANYGGNDAAARQFQ
                     SEHGIPVYKFDVGDYAACEDGVRQIVKDIGPVDVLVNNAGITRDGMLHRMKKEDWDAV
                     IRTNLDSVFNMCRLVIESMRARNFGRIINISSINGRKGQIGQTNYSASKAALLGFSKA
                     LAQEGAAKGITCNVIAPGYIATEMVAAVPKEVLETKIIPQIPVGRLGTAEEVARCVTF
                     LAADEAGFITGACLDANGGQYMA"
     gene            complement(6427..7269)
                     /locus_tag="VF226_07190"
     CDS             complement(6427..7269)
                     /locus_tag="VF226_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7073811.1"
                     /translation="MRVAETYGPYEPRGTGRLLESIAAWMLAILWILPLAYAVWTAFH
                     PPEFSVRFDPTAPLTFDNFTRAWSAAPFARYFLNTIILVALVLASQLVLCTLAAYAFA
                     RYEFPGSSIAFALVLAQLMIMPDVLIVENYSTLNTLGLVDSIPAIALPYVASAFGIFL
                     LRQTFKTVPKELDDAARVEGASALQTLLKVYVPLAKPVYLAYGLVSVSYHWNNFLWPL
                     IITSSVESRPLTVGLQVFSSTDQGIDWAIICAATLMTSAPLLVGFLLFQRQFVQSFMR
                     AGIR"
     gene            complement(7266..8141)
                     /locus_tag="VF226_07195"
     CDS             complement(7266..8141)
                     /locus_tag="VF226_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7073812.1"
                     /translation="MKRSAAAIHGWLLLLPAMAILALFTHWPAFATLVDSFYSTPRPR
                     RPQKFVGLDNYGYLLSDPVFWQAVSNNLWFAAWTIPASIGLAMLMAVLVNDRIFARGF
                     VRMAYFTPTVLPMIAVANIWLFFYTPQYGLLEQILGLLGFPSHNWLGSKDTALAAVTM
                     VAIWKEAGFFMIFYLAALQQIPPNLSEAAALEGASRWTFFRRVQFPLLMPTTLFVLIN
                     AVINAFRMVDHLFVLTRGGPDNATTLLLYYIYEVGFSFWDTAYAASLTAVLLAVLAFT
                     ALVQYGVLEKRIHYR"
     gene            complement(8190..9461)
                     /locus_tag="VF226_07200"
     CDS             complement(8190..9461)
                     /locus_tag="VF226_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073813.1"
                     /translation="MRRREFLKGTAAAAGAVIAAPAIAQSKLEISFYYPVAVGGPITK
                     IIDGYARDFEKENPGIKLNPIYAGTYQETIVKALTAHKSGVPPVMSVLLSTDMFTLID
                     EDAILPIDEFVKTDEDRKWLSSFFPAFMLNSQTGGKTWGVPFQRSTVVLYWNKELFKE
                     AGLDPDTPPANWTEQVEFAKKLTKDGQWGIQVPSSGFPYWLFQGFTTQAGAILMNQEG
                     NETYFDKPEVIEALEYWVSLSREHKVHPPGIVEWGTTPKDFFERKCAMMWTTTGNLTN
                     VRNNAKFPFGVAMLPAGKRRGSPTGGGNFHISSKASKEEQEAAFKFIQWCTSPERAAQ
                     WCVDTGYVAVRPDSFETPALKKYVEDFPQAAVARDQLEFAVAELSTHENQRVTKALND
                     GLQAALTGSKTPEQAMKDSQAEADRILRAYR"
     gene            complement(9508..10545)
                     /locus_tag="VF226_07205"
     CDS             complement(9508..10545)
                     /locus_tag="VF226_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073814.1"
                     /translation="MASVELRGVKKSWGTSVALEGVSFSAEAGSLVALLGPSGCGKST
                     TLRLIAGLETATAGEILINGKDVTDLPPAKRGVSMVFQSYALFPHLSVAENILFGLKV
                     RKVPAAERRERLKRAADILGLEHLLDRKPSQLSGGQQQRVALGRAIVAETPVCLMDEP
                     LSNLDAQLRQEMRREIRALQQRLGITMLYVTHDQVEAMTMADQVVLMRAGHIEQDDTP
                     EMLYERPATIFTGRFIGTPPMNILPYSRIEDPDGWLARLNAPGRDPSTFALGVRPEAM
                     RIEDQGVPATVASVEYLGADSLIEARMGGESIIVRASGNKRARPGDVLRLAWDAGATH
                     WFDLSSGIRIG"
     gene            10839..12089
                     /locus_tag="VF226_07210"
     CDS             10839..12089
                     /locus_tag="VF226_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7073815.1"
                     /translation="MSIRDVAAEEGRPSEGQVESRYAWMRLIASLVIATIGNVGMWAV
                     VVVLPAVEAEFGVARADASLPYTVTLIAYSASGILVGRLADRIGIVYPLVGSAIVLGL
                     GYMLTSQAGTLWQFAILHALLVGAGSSATFAPLLADISHWFTRHRGIAVAICASGNYM
                     AGTVWPPLVQRFMEAYGWRSTFFGVGIFCLVTILPLVLVLRRKPPHHQLSEAAVAAAG
                     SQARLGISPNFLMALLALAGVACCVAMSMPQVHIVAYCGDLGYGPARGAEMLSLMLGL
                     GVVSRLASGWIADRIGGLATVLLGSTLQAVALFLYLPFDSLPSLYVISGLFGLFQGGI
                     VPSYAIVVREYFSPREAGTRVSIVLMSTTIGMALGGWLSGALFDLTGDYQAAFINGIL
                     WNVLNIAIILWLLMRKRSMRMAPA"
     gene            complement(12190..12702)
                     /locus_tag="VF226_07215"
     CDS             complement(12190..12702)
                     /locus_tag="VF226_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7073816.1"
                     /translation="MRSLAVLVLALLSSGLAHAEPDNPITDCNQFKDTARQIRGCTTY
                     IRSGKALGPNLAVAYTNRGIAYASLGRHEQALADFTEAIRLAPNAPYPYYNRGNAYYD
                     LGRFDEALADYSAAIERGPDMALAYYNRGLTYQKLGNRDRSIEDFQKALTLDPGSQAA
                     RDRLNKLGVQ"
     gene            12988..13836
                     /locus_tag="VF226_07220"
     CDS             12988..13836
                     /locus_tag="VF226_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7073817.1"
                     /translation="MKLVRFGAPGAEKPGLVDADGTIRDVSAHVQDFDGSNLSPASLD
                     KLRQLDLKSLPAAPAGVRIGAPVARPMNFIAVGLNYEDHAKETGSPIPTEPILFNKLA
                     NCIIGPNDDVMIPKGSQKLDWEVEIAFVIGTRARYVEEKDALRHIAGYAICNDVSERY
                     FQIERGGQWMKGKCFETSGPLGPWLVTPDEIEDVQNLDMWLDVNGKRMQTGNTRTMIF
                     SIKYLVHYISQFMVLEPGDVVTTGTPPGVGLGMKPPVFLKAGDVMTLHIQGLGEQSQK
                     VVPFKE"
BASE COUNT         2548 a         4376 c         4445 g         2622 t
ORIGIN      
        1 aagtccgcca cattgttcgc ggctggctac gttagcggac ttctcgatct gaatcacact
       61 aggatcaata acttggctag tgtccttcag atcgcgaatt cgttccgagc tagccgcata
      121 tggtgacgaa tttcgcgatc aggacacgag cccgcattct gctgcagcca ggagcaaccc
      181 acgctataac actcgccgat gggagccggt ccataagtat ggctttgcag gacaaagtgc
      241 gtgaggtgct cgcgggatcg ccgtcccgcg gggcaaatga ggagagttgg gcggcccggc
      301 tcgccagtca cgcgccctcg tgcgcgcggg tggcgcttat cggcgggtcg ctcgccattg
      361 cgggcgccct cggcgccttc atcgtgctga gctcagtatt gctgctcttc ttccggttcg
      421 tcgacccgcc gggctcgaca ctcatgctcg gacaggcgct gaccggcggc aacgtcgagc
      481 agcgttgggt ggatctcgaa gatatctcac cgaacctcgt ccgggccgtg atcagctcgg
      541 aggacgggca gttctgcacc cactacggcg tcgacctggg ggagcttgcg gcggcattgc
      601 gcaaagccga gcgcagcggc ctcgatgacg cccgcggcgc gagcaccata accatgcagg
      661 tggcgaagaa cctgttcctg tggccatcga gagatctcct gcgcaagggt ttggagatcg
      721 gaatggcgct gatcatggag cagatctggc cgaagcgacg aattctcgag gtctatctga
      781 acatcgtgga gtggggacca ggcattttcg gagccgaggc ggctgcgcgg catcatttca
      841 agaagccggc gtcgcggctg acggcacgcg aggccgcgct gctggcggct tccctgccga
      901 atccgcacaa gcgcatcgca agcaggcccg gaccccacac acgccggatc tcctacatcg
      961 tccaggcgcg ggcgagatcg gccggccagc gcacatcttg tgtcctgccg gtcgagggtc
     1021 gcacctgagc ttcgggtgct ggcgttcgtc acgaaatccg gttataagcc cgcgcgaaga
     1081 ctgcccggtg ccctgtgccg ggcagcaggg attcgtttac tccatcatac gaatttgaca
     1141 gtttcggccc gcccgccgga ccgatcgaag gagacaggca tggctgtacc ccgcaagaag
     1201 acgtcgccgg caaagcgcgg ctcccgccgg tccgctgacg cgctcaaggc tccgacctac
     1261 atcgaggaca aggagagtgg tgagcggcgc cgcccccatc acatcgacct caagaccggc
     1321 aagtaccgcg gtcggcagat cctcgaagcg aaagaccaag tctaaagcga gaccggtccg
     1381 cggggggagg cggcggccgc gccgctgggc ttaggctccg gctgacataa tttcagcttc
     1441 cacccccgat atggtcgatc ggctcagatc cggaggtgtt cgatgtcgct gagagccatt
     1501 ccgctgattg ccgtggcgtt gatcctgtac aacgccattg ttctactggg cggcagcgag
     1561 acgcctagcg agattctgag cacccgcctg ttcgaaatcc cgatgctgca cgagaacgcg
     1621 gtctggatct tcacgtgggg tgacttcctg atcctgctgg tgctcgtgct gctgttcatc
     1681 gagctcttga aggcgacgta cacgacgacg gcctccctgc tcgatcacgg cctctccatg
     1741 atcgtgttcg ttgtttgcct gatcgagttc ctgctggtga ggcaggccgc gagctcggtc
     1801 ttcttcttca tcacgatggc agccctcatc gatgtggtcg cgggctatac gatcggcatc
     1861 cgtgtcgccc gccgcgactt ccagatcggc gagtgacggc atggccggcc ggcgattttt
     1921 cgccggcttt cagagcttgt taggcagtag cccctaacct cgaccctctt tggagttgtc
     1981 gaggtcaggc gtgaccaggt taatcaagct gctgccagtg ccggcggccg gcgctggaga
     2041 gcaatcagcc gagcgcgacg tccccctccc ggcgcggcct gatgatgtgt ctgccggttg
     2101 cggcgaggat cctcacgcgc tggctcaact cgagcgcgac gagctgacgt gcctcgtcgc
     2161 ccgaaggcac ctcgtcgagg cgctggatca cattctggcg ggcgacgggc aggagccgtg
     2221 ggcatttctc ctgatcggca tcaacgacct ccctgtcacc aacgagatgt tcggcttcga
     2281 ggtcggcgac gagctgatcg ccgcggtggc tcacgtgctc accgctgagc tcgaggaggg
     2341 atggctgctc ggccggtatt cctccaacaa attcggtctg ctcgttccag cggcgagcgc
     2401 agaggagatg cgcaagacgg ccgagcgcct gtcgcgagca gtcagggagg cgcggctcga
     2461 gacctcggcc tgccgcctgg ccacgacgat atcgatcggc ggcctgcttc ttccgcagca
     2521 ggccgcgagc agcagcgagg cgctgagccg ggcccagggc gccttgtccc gctcgagata
     2581 cgggtcgttc ggtgggttcg cggcctatga tcccggctgg gctcacgatt atgtgcgcgg
     2641 ccgcaacgtc cgcattgcca gagatgtcgt gtcggcgctc gaaagcggcc gcatgcggct
     2701 cgcgctgcag ccgatcgtct cgacagccac gtggcagccg gtgttccatg aatgcctgct
     2761 gcgcatggag ctgcccgagg gccgaacgat cgctgccggg aaattcatcg gtgtcgccga
     2821 gcagctcggc ctggcccgcc tggtcgatcg gcgcgcgctg gagctggcca tcgatctcct
     2881 ccatgatagg ccggatctgc atctcgctct caacgtctcg gggctgacgt gcaccgatca
     2941 tgattggctg gtcgccctgc aagggctgac gagcgccgga gggctggcgg agcggctcac
     3001 gatcgagatc accgagacgg cagccatcca ggatttcgac cactccgtca cattcgtcga
     3061 cgcgttgaaa gagcttggct gccgggtcgc catcgacgat ttcggcgcgg gctacacctc
     3121 gttccggacc ttgaagcacc ttgccatcga catggtgaaa atcgacggcg cattcgtccg
     3181 gaccctgccg cgggacagca cagatcaagt gttcatcaag gcgatggtcg agcttgcccg
     3241 cagcctcaac ctttcgactg tggccgagtg ggttggcgac gaagcaaccg cccgcctgct
     3301 agctgatatc ggcgttgact acttgcaggg cttccatttc ggagagccgg tggttttcgg
     3361 agcgaatagc gagtagcgaa tggtgaatag gggatacctg cagccccaat ctccctttgc
     3421 tcattcagcg cttctgagac agcttctcaa tcttttcctg catcgaggca agctgcgcct
     3481 tgaggtcctc gagttcggac ttggtggcct cctctgagcc agccttctcc tgagctggct
     3541 cggcgccctt ggcggcgccc gcctgatccg cttcctcctg cggcttcgga cccggagcct
     3601 gcgtgccgaa cggcgtccac attgccatgg ccttctcgaa catggccatg ttcttgcggg
     3661 tctgctcctg catcgcatcg agcgcggcgg caccgaatgg gctctgaccg agcatgtgcg
     3721 agaattgcgc gcggaagcgc tcctgctcct tcgccagcgt ttcgaggctc atctgcagat
     3781 agctcggcac catgcgggcg atgctgtcgt cgtagaagcg gatcagctgg cgcaggaacg
     3841 ggatgggaag cagcgtctgt ccgcctgaac ggccctcctg ctcgacgatg atctgcgtca
     3901 gcacggagtg cgtcaggtca tcgccggttt tggcgtcgta aacgacgaaa tcgcgatcgg
     3961 atctcaccat tgcagcaaga tcctccagcg tcacgtacgt gctcgtctct gtattgtaga
     4021 gacgccgatt ggcgtacttc tttatgacca ccgcctcctt cttgtcggcg ggagggtcgc
     4081 ttttgctgag catggcgatc agatcttggc agtggctgca tttgacaagc tagcacagtg
     4141 ctcaaccgtg gcaacatatt aatcgccctc ggccgatcgc catactgcac tgcatcaaac
     4201 gactcattgc gggctgcgac ggcaggctgc tatcgtagcc agcaatcgga ttcccttcca
     4261 atcagggcgg tgggagccca cgttctgatc acggcgaacc gccactacca gtccgggtca
     4321 tcccaggaga gcgataatga gcgcagacaa ctcgacaatc gtgatcgcga gcgccgctcg
     4381 cacgcccgtg ggttcattca atggctcgct ttcgagtctt ccggcgcacg agctcggcaa
     4441 agttgccatc aaggcggcca tcgagcgggc cggtatcgag ccgggtgagg tcgacgaggt
     4501 catcatgggc cagatcctta ccgccggcgc tggccagaac ccggctcgcc aggcatcgat
     4561 cgccgccggc attccagtcg acagcccggc ctggggcatg aaccagctct gcggctccgg
     4621 gctcagggcc gtcgcgctcg gtgcccagca gatccagctc ggctcctcgt ccatcgtcgt
     4681 ggccggcggt caggagagca tgagccaggc gccgcactgt gcgcacctgc gcgaaggcac
     4741 gaagatgggc gacctgaagc tcatcgacac gatgatcaag gacgggctgt gggatgcctt
     4801 caacggctac cacatgggat cgacggcgga gaacgtcgcc cggcagtggc agatcacacg
     4861 cgaggagcag gacaggttcg cggtcgcgtc ccagaacaag gccgaggctg ccaagaaggc
     4921 cggcaaattc aaggacgaga ttgcccccgt caccgtcaag acgcgcaagg gcgagacggt
     4981 cgtatccgag gacgagtaca tccgcgaagg cacgacgtac gaggccgtcg caaagctgcg
     5041 ccccgcgttt gagaaggacg gcacggtgac ggctggcaat gcgtccggaa tcaacgacgg
     5101 cgctgcagcc gtggtgctga tgacgctggc tgaggccgag cgtcggggca tcacgccgct
     5161 ggcgcgaatc gtgtcgtggg cgcacgcggg cgtcgacccg tccatcatgg gcaccggacc
     5221 cattccggct tccaagaagg cgctggagaa agccggctgg tctgtcgcgg acctcgacct
     5281 catcgaggcc aacgaggcgt tcgccgccca ggcctgtgcc gtcaacaagg gcctcgggtg
     5341 ggatacggag aaggtcaacg tcaacggcgg cgccatcgct ctcggccacc cgatcggtgc
     5401 ctccggtgcg cgcatcctca acacgcttct tttcgagatg aagcggcgcg atgcgaagaa
     5461 gggcctcgca accctctgca tcggtggcgg catgggcgtc gccatgtgcg tggagcgtgt
     5521 ctgaagccgg tccgaaagac gggaaaatct gataggatgg ctggcggagc ctgctccgcg
     5581 gcctgattta tgacggacat caatccgtcg ttgacaatat catcctatca tttcgctttc
     5641 acgtcttgga ggacaccatg gcacgtgtgg cggtcgtcac gggaggcaca cgcggcatcg
     5701 gcgctgcgat atccattgcg ctcaagaaca aaggctaccg ggttgcagcg aactacggcg
     5761 gcaatgacgc cgcagcgcgc cagttccaga gcgagcacgg aattccggtc tacaagttcg
     5821 atgtcggaga ctatgccgcg tgcgaggacg gcgtccgcca gatcgtcaag gatatcggcc
     5881 ccgtcgacgt gctggtaaat aacgccggca tcacgcgcga cggcatgctg caccgcatga
     5941 agaaggagga ttgggacgcc gtcatccgga ccaatctgga ttccgtcttc aacatgtgcc
     6001 ggctagtgat cgaaagcatg cgtgcgcgga atttcgggcg cattatcaat atttcctcga
     6061 tcaatggtcg caagggacag atcgggcaga ccaactactc ggcctcgaaa gctgcattgc
     6121 tgggtttctc caaggctctc gctcaggagg gtgctgccaa aggcatcacc tgcaacgtca
     6181 tcgcaccagg ctacatcgcc accgagatgg tcgcagccgt gccgaaagag gtcctcgaga
     6241 cgaagatcat cccgcaaatc cccgtcggcc ggctcggcac cgccgaggaa gtggcgcgtt
     6301 gcgtgacctt cctcgcggcg gacgaagcag gtttcatcac gggtgcttgc ctcgatgcca
     6361 acggcggcca gtacatggcg tgatgcggag cctgcgctga gggtcggagc cccgttcgcg
     6421 caggcgctaa cggatacctg cgcgcatgaa gctctgcacg aactggcgct ggaacagcag
     6481 gaagccgacg agcagcggcg ccgaggtcat gagcgttgcc gcacagatga tcgcccagtc
     6541 tattccttga tcggtcgagg agaagacctg caggccaacc gtcagcgggc gggactcgac
     6601 cgagctggtg atgatcagcg gccaaaggaa gttgttccag tgatagctga ctgaaacgag
     6661 gccgtaggcg agatagaccg gtttcgcaag cggcacgtag accttgagga gcgtttgcag
     6721 cgccgaggcg ccctccacgc gggcggcatc gtcgagctcc ttcggaaccg ttttgaatgt
     6781 ctgccgcagc aggaagatgc cgaatgccga tgccacgtag ggcagcgcga tggcagggat
     6841 ggagtcgacc aggccgagcg tgttcaacgt gctgtaattc tcgacgatca gcacgtcggg
     6901 catgatcatg agctgcgcca gcacgagcgc gaaggcgatg gagctgcccg gaaactcgta
     6961 gcgcgcgaag gcgtaggccg cgagcgtgca cagcacgagc tggcttgcga gcaccagcgc
     7021 aaccaaaata atcgtgttca ggaaataccg ggcgaacggc gcggccgacc aggcgcgagt
     7081 gaagttgtcg aaggtcagcg gggccgtcgg atcgaaccga acggagaact ccggcgggtg
     7141 gaaagcggtc cagaccgcat aagcgagcgg caaaatccag aggatcgcca gcatccaggc
     7201 ggcgatgctt tccagcaggc gcccggtccc gcgcggctca taaggtccgt atgtctccgc
     7261 caccctcatc ggtaatggat ccgcttttcg aggacgccat actgaacgag ggcggtgaac
     7321 gcgagcacgg cgagcagaac ggccgtcagc gatgcggcat aggccgtatc ccagaagcta
     7381 aaaccgacct catagatgta atagagcagc agtgtcgtcg cgttatccgg cccgccgcgc
     7441 gtcagcacga acaagtgatc caccatgcgg aaagcattga tcaccgcgtt gatgagcacg
     7501 aacagcgtcg tcggcatcaa cagcgggaac tgcacgcggc ggaagaacgt ccagcgcgag
     7561 gcgccctcca gcgctgcggc ttccgacagg ttcggtggaa tctgctgcaa ggcggcgaga
     7621 tagaaaatca tgaagaagcc cgcctccttc cagatcgcga ccatggtgac ggcggcgagc
     7681 gccgtgtcct tggagccgag ccaattgtgc gatggaaaac cgagcagtcc cagaatttgt
     7741 tcgagcagac cgtattgcgg cgtgtagaag aaaagccaga tattcgcgac cgcgatcatc
     7801 ggcagcacgg tcggcgtgaa ataggccatc cggacaaaac cgcgggcgaa aatgcggtca
     7861 ttgacaagca cggccatcag catggcgagg ccgatggagg ccggaatggt ccaggccgca
     7921 aaccagagat tgttcgagac ggcctgccag aaaaccggat cgctcaacaa gtagccatag
     7981 ttgtcgaggc cgacgaactt ctggggccgc cgcggccgcg gggtcgagta gaagctgtcg
     8041 accagcgtcg cgaatgccgg ccagtgcgtg aagagcgcca gtatggccat agccggcagc
     8101 agcagaagcc agccatggat tgcggcggcg gaacgcttca ttccaaatcc ccggagtggc
     8161 ggcgggctgc ttgcccgcca cctagatcgt cagcgatagg ccctcagaat gcgatcggct
     8221 tccgcctgag agtccttcat cgcctgctcc ggcgtcttgg agcccgtgag cgccgcctgc
     8281 aggccgtcgt tgagggcctt tgtgacgcgc tggttctcgt gcgtcgaaag ctcggccacg
     8341 gcgaactcga gctgatcgcg cgcaacagcc gcctgcggga agtcctcgac gtatttcttg
     8401 agcgccggcg tctcgaagga atcggggcgg acggcgacgt agccggtatc cacgcaccat
     8461 tgcgccgcgc gttcgggcga ggtgcaccac tggatgaact tgaaggcggc ttcctgctcc
     8521 tctttgctgg ctttgctcga aatgtggaag ttgccgccac cggtgggact gccacgccgc
     8581 ttgcccgcgg gcagcatggc gaccccgaac gggaattttg cgttgttgcg cacgttggtg
     8641 aggttgccgg tggtcgtcca catcatggcg cacttgcgct cgaagaagtc cttcggcgtc
     8701 gtgccccact cgacaattcc cggcggatgg accttgtgct cgcgcgagag gctgacccag
     8761 tactctaacg cctcgatgac ttccggcttg tcgaaatagg tctcgttgcc ctcctggttc
     8821 atcaggatgg cgccggcctg cgtggtgaag ccctggaaca gccagtaagg gaacccggaa
     8881 gacggcacct ggatgcccca ctggccatcc ttcgtcagct ttttcgcgaa ctcgacctgc
     8941 tcggtccagt tggccggcgg cgtgtcgggg tcgaggccgg cctccttgaa cagttccttg
     9001 ttccagtaga gcacgacggt ggagcgctgg aacggcacgc cccaggtctt gccgccggtc
     9061 tgactgttga gcatgaaggc cgggaagaag ctcgacagcc acttgcggtc ctcgtccgtc
     9121 ttcacgaact cgtcgatcgg gaggatcgca tcctcgtcga tcagggtgaa catatcggtg
     9181 gagagcagca ccgacatgac gggcggcacg ccgctcttgt gcgcggtcag cgccttgacg
     9241 atcgtctcct gatatgtgcc cgcatagatc gggttgagct tgatcccagg attctccttt
     9301 tcgaagtccc gggcatagcc gtcgatgatc ttcgtgatcg gtcctccgac ggccacaggg
     9361 taataaaacg atatttcgag cttcgattgc gcgattgccg gcgcggcgat caccgcgcct
     9421 gccgccgcgg cggtgccctt caggaattca cgtctgcgca tttgctttca tctccctttg
     9481 ctgaagcgcg ttcggcgcgg cctttgctta tccaatgcga atgccagatg acaggtcgaa
     9541 ccaatgtgtt gcgcccgcat cccaggcgag tcgtagcacg tcgccgggcc gggcgcgctt
     9601 gtttccagaa gcgcggacga tgatgctctc ccctcccatc cgtgcctcta tgaggctgtc
     9661 tgcccccaga tattcgacgg atgccacagt ggcgggcacg ccctgatctt cgatccgcat
     9721 cgcctcgggg cgcacgccca gggcgaatgt cgaaggatcc cgccccggcg cgttcagcct
     9781 ggccagccag ccatcggggt cctcgatccg tgaatacggc agaatgttca tcggcggggt
     9841 gccgatgaac cggccggtga atatggtggc cgggcgctcg tagagcattt ccggcgtatc
     9901 gtcctgctcg atgtgcccgg cccgcatcaa cacgacctgg tccgccatgg tcatggcctc
     9961 cacctgatcg tgcgtgacgt aaagcatggt aatgccgagc cgctgctgca gggcgcggat
    10021 ttcgcgccgc atctcctgcc ggagctgggc gtcgagattc gacagcggct cgtccatcag
    10081 gcagacgggc gtttcggcga cgatggcccg ccccaacgcg acgcgctgct gctggccgcc
    10141 ggagagctgt gacggcttac gatcgagcag gtgttcgagc ccgaggatgt cggcagcccg
    10201 cttcaggcgt tcccgccgtt ccgctgccgg aaccttgcgc accttgaggc cgaagagaat
    10261 gttctcggcg accgacagat ggggaaacag ggcgtaggat tggaacacca tcgaaacgcc
    10321 gcgcttagcc ggcggcaggt cggtgacgtc cttgccgttg atgaggattt caccggcagt
    10381 tgcggtttcc agcccggcga tgagcctgag agtggtggac ttgccgcaac ccgagggtcc
    10441 gaggagtgca acaaggctgc ccgcctcggc actgaagctc actccttcga gcgcgacgct
    10501 cgttccccag ctcttcttaa cacctctgag ctcgacgctc gccaacgatg atctccccgg
    10561 cgtttcctca ttgcctccct tatgaggcgc ttgcccggcg cgttcaaatc cgaaactctc
    10621 gacaggatgt caacctgtcg gttgcaacag cttcgacctc aagcaccatc ctcgacgccg
    10681 tgtgagaccc gtccatccgc gacaggcagc taattcggct cgcccgcgac cggcatttgg
    10741 gctaagtctg ccttgtcaca gtgcagttca caatggcggc cccggcgcat tcgcggcagc
    10801 cctcgaaaag atgatctgag caaggagttg cgggtgtcat gagcattcgt gatgtggcgg
    10861 ccgaggaagg ccgtccgagc gaaggccaag tcgagtcccg ctacgcctgg atgcggctga
    10921 tcgcatccct ggtgatcgcc acgatcggaa atgtcggcat gtgggccgtc gtggtggtgc
    10981 tgccggcggt ggaggcggag ttcggcgtcg cgcgcgccga cgcgtctctc ccctataccg
    11041 tgacgctgat cgcctactcg gcgagtggca ttctggtggg ccgtctcgcc gaccggatcg
    11101 gcatcgtcta tcccctcgtg gggagcgcaa tcgtgctcgg cctcggctac atgctgacga
    11161 gccaagccgg cacgctctgg caattcgcca ttcttcacgc gttgctcgtc ggcgccggca
    11221 gctccgcgac cttcgctcca ttgctggccg acatttccca ttggttcacg cgacaccgcg
    11281 gaattgccgt agcgatctgc gcgagcggca attacatggc cggaaccgta tggccgccgc
    11341 tcgtgcagcg cttcatggag gcttacgggt ggcggtcgac ttttttcggc gttgggatct
    11401 tttgtctggt gacaatcctg cccttggttc tggttcttcg ccgcaaacca ccgcaccatc
    11461 agctttcgga ggctgcagtc gccgcagcag gatcacaggc gcggcttggt atctcgccga
    11521 acttcctcat ggccctactg gccctggcgg gcgtcgcctg ctgcgtcgcg atgtcgatgc
    11581 cacaagtgca catcgtggcc tactgcggtg acctcggcta cggaccggcg cgcggcgcag
    11641 aaatgctgtc gctgatgctc ggcctcggcg tcgtcagccg cctcgcctcg gggtggatcg
    11701 cggatcgcat cgggggactc gcgacggtgc tcctcgggtc caccctgcaa gccgtggcgc
    11761 tcttcctcta cctgccgttc gacagccttc catcgctcta cgtgatctca ggtctgttcg
    11821 ggcttttcca gggaggcatc gtcccgagct acgccatcgt cgtgcgcgaa tacttctctc
    11881 cgagggaagc gggcacccgc gtcagtatcg ttctgatgtc gacgacgatc ggcatggcgc
    11941 tcggcggctg gttgtcgggc gcgctcttcg atttgaccgg cgattaccag gcggccttca
    12001 tcaacggcat tctctggaac gtgctgaaca tcgcgatcat tctctggctg ctgatgcgca
    12061 agcgcagcat gaggatggct ccggcctgat caggcacgag ccaatttgcg atcgagtgcc
    12121 aagccaaacg aggcttcctc gcaatccctc cttgggagaa gtcggtacgg gggcctgatc
    12181 gggtaaggtt cactgcacgc cgagcttgtt cagccggtcc cttgcggcct gcgagccggg
    12241 atcgagtgtc agcgccttct ggaagtcctc gatgctccgg tcccgattgc cgagcttctg
    12301 atacgtcaga ccccgattgt aataggcgag cgccatatcc ggcccgcgct cgatcgcggc
    12361 ggagtagtcg gcgagcgcct cgtcgaacct gccgagatcg tagtaggcat tgccccgatt
    12421 gtagtagggg tagggcgcgt ttggggctag acggatcgct tccgtgaagt cggcgagagc
    12481 ttgctcgtgc cgccccagcg aggcataggc gatgccgcgg ttcgtatagg cgaccgccag
    12541 attcggcccg agagcctttc cgctgcgaat gtaagtcgtg cacccgcgga tctgccgggc
    12601 cgtatccttg aactggttgc agtccgtgat cggattgtcc ggctcggcgt gcgcaagccc
    12661 cgagctgagg agtgcgagaa cgaggacggc gagcgatctc aaaggtgatc tccggtgcaa
    12721 tgctgcggct cgatcatggc cgattccctg agcacacccc acccccagtg ccccaatgat
    12781 atagggagtc gggccagcca acgccgcacg gtgtggcaaa tttctcgatc gtcacgctaa
    12841 ggcaccttcg accctgcgcc ggcaggcaga tgccagtctg caagcgcgtc cgcggttcct
    12901 tcgccccgct caccctcttt acgctcgggt ccattcggcc taccgataga gcctaccaca
    12961 acaggaccgt tctaggagcg aaaactcatg aagcttgtgc gatttggcgc gccaggtgcg
    13021 gaaaagcccg gcctagtcga tgcagacggc acgattcgcg atgtctcggc gcacgtgcaa
    13081 gacttcgacg gctcgaacct cagccccgcc agcctggaca agctgcgcca actcgacctg
    13141 aaatcgctgc cggccgcccc cgctggcgtg cgcattggcg ctccggtcgc ccggcccatg
    13201 aacttcatcg ccgtcggcct caactacgag gaccacgcca aggaaaccgg ctcaccgatc
    13261 ccgaccgagc ccattctgtt caacaagctc gccaattgta tcatcgggcc gaacgacgac
    13321 gttatgatcc cgaagggctc gcagaagctc gactgggagg tcgagatcgc cttcgtcatc
    13381 ggcacgcgcg cccgctacgt ggaggaaaag gacgcccttc gccatatcgc cggctacgcg
    13441 atctgcaatg acgtctccga gcgctacttc cagatcgagc gcggtggcca gtggatgaaa
    13501 ggcaagtgct tcgaaacgtc cggcccgctg ggtccctggc tcgtgacgcc ggacgagatc
    13561 gaggatgtgc agaacctcga catgtggctc gacgtgaacg gcaagcgcat gcagaccggc
    13621 aacacgcgga ccatgatctt ctcgatcaag tacctcgtgc actacatctc ccagttcatg
    13681 gttctcgagc ccggcgatgt cgtcaccacc ggcacgccgc ccggcgtcgg tctcggcatg
    13741 aagccgcccg tgttcctcaa ggcgggcgac gtcatgaccc tgcatatcca gggcctgggc
    13801 gagcaatcgc agaaggtcgt gccgtttaag gagtaagcgg tccggctgcc gtatcagccg
    13861 acgcctaatt catcgtctcc gggtgaggga ttccgagaac tcctactgtc atatcccctc
    13921 acccgaccct tcgggtcgac ctctccccat gggagaggtg aagcgagtgc ctgctgccgt
    13981 tccagcttcg c
//
LOCUS       DASVDU010000113         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1219168, whole genome shotgun sequence.
ACCESSION   DASVDU010000113 DASVDU010000000
VERSION     DASVDU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219168"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VF226_07225"
     CDS             <1..786
                     /locus_tag="VF226_07225"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX7073818.1"
                     /translation="RRHELIDDPEAALARVSAAGDLENAVAGADLVQESTPETLEMKR
                     EIFARLDSAAPPEAILASSTSSLVTSLYTENLAGRSRCLVAHPVNPPHLVPIVELCGA
                     PWTSPEVMQRAREIYRSVGQVPIHVKREVDGFVLNRLQTALLAESFRLVEEGVVTPQD
                     LDETIRNGLGLRWAFMGPFETIELNAPGGIPDYCRRYSPFFRRLAADQPKASVWDDDK
                     TAEAAAAWGPIPDPQKHAAKTRWRDEQLAALAAHKRRMKPFTD"
     gene            800..1732
                     /locus_tag="VF226_07230"
     CDS             800..1732
                     /locus_tag="VF226_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX7073819.1"
                     /translation="MSKSRKVIITCAVTGSIHTPSMSPHLPITPEEIADAAVGAAEAG
                     AAIVHLHARDPQTGKPDQSVEAFAPFLKVIKQRSGCVVNITTGGAMTMTVEERVRPAA
                     TFKPEIASLNMGTMNFGLYPMLARTKEFKFDWERPYLEGSRSGFFKNTFSDIEYILKT
                     CAGNGTRFEIECYDIGHLYTLAHMVDRKIVEPPLFVQSVFGILGGIGPHPEDVAHMKR
                     TADRLFGKDYQWSVLGAGRNQLPIAAMAASMGGNVRVGLEDSLWAGPGKLAESNAQQV
                     QLARQIIEGLGLEIATPDDAREMLQLKGGDQVAF"
     gene            complement(1832..>2123)
                     /locus_tag="VF226_07235"
     CDS             complement(1832..>2123)
                     /locus_tag="VF226_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073820.1"
                     /translation="NLDESRAVYNWVVELCVKLGADEKDMVPFEKYANAALSLGSPSS
                     AARALAAGAPNIERVDRLVQIIAAQKGMKSEALDRTVKLVDGWLEKNRQKAS"
BASE COUNT          393 a          671 c          673 g          386 t
ORIGIN      
        1 aggcggcacg agctgatcga tgatcccgag gcggccttag cccgcgtcag cgcagccggc
       61 gatctcgaaa atgccgtcgc cggggctgat ctcgtccagg agagcacgcc tgaaacgctg
      121 gagatgaagc gggagatttt cgccaggctc gattctgcgg ccccgcctga ggctattctc
      181 gcatcctcga cgtcctcgct cgtcacctcg ctttatacgg agaatcttgc cggccgcagc
      241 cgttgcctcg tggcccatcc cgtcaatccg ccgcatctcg tgccgatcgt cgagctatgc
      301 ggtgctccgt ggacgtcgcc tgaggtgatg caacgcgcac gagagatcta tcgctcggtc
      361 ggtcaggtgc cgatccatgt caagcgagag gtggatggct tcgtgctgaa ccggctgcag
      421 acggcccttc tcgccgaatc gttccggttg gtcgaggagg gcgtcgtcac cccgcaggac
      481 cttgatgaaa cgattcgaaa cggactgggc ctgcgctggg ccttcatggg cccgttcgag
      541 acgatcgagc tcaatgctcc cggcggcatt cccgactatt gccggcgtta cagcccattt
      601 ttccgccgcc tcgccgccga tcagccgaaa gcgtccgtgt gggatgatga caagacggct
      661 gaggccgctg cggcttgggg accgatcccc gatccgcaga agcacgccgc caagacgcgg
      721 tggcgcgacg aacagcttgc cgcactcgcg gctcacaaac gcagaatgaa acccttcact
      781 gactgaggag gccggaacca tgagcaaatc ccgcaaggtc atcatcacgt gcgccgtaac
      841 cggctcgatc cacacgccgt ccatgtcgcc gcatctgccg atcacgccgg aggagatcgc
      901 agatgccgcc gttggcgcgg ccgaggcggg cgccgctatc gttcacctgc acgcacggga
      961 cccgcagacg ggcaagcccg accagagtgt cgaggcgttc gcgccgttcc tgaaggtgat
     1021 caagcagcgt tcggggtgtg tggtgaacat caccaccggc ggcgccatga ccatgacggt
     1081 cgaggagcgg gttcggcctg cagcgacttt caagccggag atcgcatcgc tcaacatggg
     1141 cacgatgaac ttcggcctct atccgatgct ggcgcggacg aaagagttca agttcgactg
     1201 ggagcggccc tacctcgagg gctcacgcag cggatttttc aagaacacct tctcggacat
     1261 tgagtacatc ctgaagacct gcgccggaaa cggcacgcgg tttgagatcg aatgctacga
     1321 catcggccac ctttacacgc tggcccatat ggtcgaccgc aagatcgttg agccgccgct
     1381 gttcgttcag agcgtcttcg gcattctcgg gggaatcggc ccgcatcccg aggatgtcgc
     1441 gcacatgaag cgcacggccg atcggctgtt cggcaaggac taccagtgga gtgttctggg
     1501 cgctgggcgc aatcagctcc cgatcgcagc catggcggca tcgatgggtg ggaatgtgcg
     1561 cgtcggcctc gaggattcgc tgtgggctgg ccccggcaag ctggccgaaa gcaacgccca
     1621 gcaggtgcaa ctcgcacgtc agatcatcga ggggctgggg ctcgaaatcg ccaccccgga
     1681 cgatgcgcgc gagatgctgc agctcaaagg cggagatcag gtggcgttct agcctggtat
     1741 ccaagctgga gatgcccgcc ccctctcccg tgtgtggcga gagggcggga ctgcttcatc
     1801 tgaccgttct ataaagcagt acgaataagc gtcagctcgc cttctgccgg ttcttctcga
     1861 gccagccgtc gaccagcttc acggtgcggt ctagcgcttc ggacttcatg cctttctggg
     1921 cggcgatgat ttgcacgagg cggtcgacgc gctcgatgtt cggcgcaccg gcagcaagcg
     1981 cacgggccgc ggacgaagga ctcccgaggg acagggcggc gttggcgtat ttctcgaaag
     2041 gcaccatgtc cttctcgtcc gcgccgagct tgacgcacag ctccacgacc cagttataaa
     2101 ccgcccgcga ctcatcgagg ttg
//
LOCUS       DASVDU010000114         3513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_862647, whole genome shotgun sequence.
ACCESSION   DASVDU010000114 DASVDU010000000
VERSION     DASVDU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862647"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..90)
                     /locus_tag="VF226_07240"
     CDS             complement(<1..90)
                     /locus_tag="VF226_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX7073821.1"
                     /translation="MNMFKRFVRDESGATAIEYGLIAALIAVAI"
     gene            614..1126
                     /locus_tag="VF226_07245"
     CDS             614..1126
                     /locus_tag="VF226_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HEX7073822.1"
                     /translation="MPACCGNRVRILCHSAVVALAFALASSGIFFRAARAEDLIVKYD
                     QSQLWRLDRPVAEIIIGNPTIAEVTIQSSDLLVVTGKSFGITNIIALDADRKIVAERR
                     IIVQRDQLKVVNLLKGSKRETYNCTPQCNPSITIGDDSQYFETIAKLSEQKMKLSSGN
                     AGSGGGDSAN"
     gene            1274..1846
                     /locus_tag="VF226_07250"
     CDS             1274..1846
                     /locus_tag="VF226_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7073823.1"
                     /translation="MSQGDGFIRRLRRCRLIQLARQLEQDRSGTTAIEFAMVGLPFFM
                     FLFGIFGLGLYFFTTFSLENAVEQASRLIRVGLVQQSGMTATEFKQEVCKRLPPFVDC
                     NEKMRVNVQNYADFGSITAPSCTDSQGNLVPPDGTTFRTGGSSEVVLVTVCYEWDLAG
                     VLPFLKLGNMPNGAALIQASATFLTEPYED"
     gene            1850..2443
                     /locus_tag="VF226_07255"
     CDS             1850..2443
                     /locus_tag="VF226_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX7073824.1"
                     /translation="MRRSGRSRNRSRAAATFTRMLGDRRGIAAVEFALILPIMFMLLV
                     GTVEFSLALTVDRRVSQVANSTADLIAQKKKITASEVAGIMKIIEHLMRPYDPSRLRV
                     TVLNVIANISDASDTTVCWSYEHNGGAGSYAAGAEYALPENIVEAGGSVIVAEVVYDY
                     EPIIFKYFIKSAFTLRDTLYLKPRLSSYVEYNGQPCS"
     gene            complement(2477..3340)
                     /locus_tag="VF226_07260"
     CDS             complement(2477..3340)
                     /locus_tag="VF226_07260"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7073825.1"
                     /translation="MTEARLQELLGALTSTVPPRLAEAMRYAVLGGGKRFRPFLVIES
                     ARLFGLAPEGAVDAAAAVELVHCYSLVHDDLPAMDDDDMRRGRPTVHVAFDEATAILA
                     GDALLTFAFEVLADRGAHPDPEVRCELASALARAAGPAGMVGGQQLDLEAEKLPRQGA
                     GDLDRVRDIQQRKTGALIAFAAEAGAILASAGEEERRALSSYGQALGAAFQVADDLLD
                     VEGAAAVVGKATGKDAAAGKATYVSALGVEGAREQLNRLQAEAAAALQRFGGKADVLR
                     QAVTYVATRTS"
BASE COUNT          710 a         1093 c         1032 g          678 t
ORIGIN      
        1 tatcgccacc gcaatcaacg ccgcgatcag cccgtattca atggccgtag cgccggattc
       61 atcacgcacg aagcgcttga acatgttcat atgactcatc tcccactcgt gattggcgcc
      121 tccgcgccat gttctgctgc atttgagccg aacacgagga aaattaggtc ggagatgttt
      181 ccgagaagta aaaccaaaac aacagtcagc gatgcttgtt gcaactcaag cgcgatgttc
      241 tgaaaaacaa agaataatag ggacactttt tgcgtgtatt acaaagtaag acaagtagaa
      301 taagcccgac atgcctgtcc tccgacgctc caatgtggct aattttgggt gaacctcgcg
      361 cttcgaccag tagacagcaa ggttgacatc gagtcaggtc gctcacaggg ctcacgacaa
      421 atcgacagcc tggcgtgatc tggctgcagc attgcttggc ggatcggcag ccagttcgct
      481 gcggcacggt gggtcgctct atttgttcgg cagtgaaagc agcgaagtcg ccctaacccg
      541 aaaccgccat ttccgattcc ttcacgaata gctggcctaa tcgccccgga cgagcaaatc
      601 gaggggagag atcatgcctg cctgttgcgg gaatcgtgtc cgcattctct gccattcggc
      661 cgttgtcgcc ctcgcgttcg cgctggcaag ctcagggata ttcttccggg ccgctcgtgc
      721 cgaggatctg atcgtgaagt acgatcaatc gcaactctgg cgtctggacc gaccggtggc
      781 cgaaatcatc atcggcaacc ccaccatcgc cgaggtgacc atccagagca gcgatctgtt
      841 ggtcgtgacg ggcaagtcgt tcggcatcac gaacatcatc gccctcgatg cggatcgcaa
      901 gatcgtcgct gagcgccgca ttatcgtgca gcgggaccag ctcaaagtgg tcaacctgct
      961 taaagggagc aagcgcgaga cctataactg cacgccacag tgcaatccat ccatcacgat
     1021 cggggacgac tcgcaatact tcgagacgat cgccaagctg tccgagcaga aaatgaagct
     1081 gtcatcaggc aatgctggaa gcggtggcgg cgacagtgcg aactgacctt tggtcccgcc
     1141 gctgcatgcc caccgagcac ggctgtcaac gatcctttaa ccgactcgca acctttacga
     1201 aggttcgcaa caatccgttc agatttgttc aatcacctag ctctctcaag tgattaagtt
     1261 gacgagagcg gtcatgtccc agggtgacgg tttcatccga cggctgcgtc gatgccgcct
     1321 catccaactt gccaggcaac tggagcagga cagaagcggc accacagcca tcgagttcgc
     1381 gatggtcgga ttgccgtttt tcatgttcct gttcggcatt tttggtttgg gcctctactt
     1441 cttcacgacc ttctcgctcg agaacgccgt cgagcaggct tcgcggctga tccgcgtcgg
     1501 cctcgtgcaa cagagcggga tgacggcaac ggaattcaag caggaggtct gcaagcgcct
     1561 gccgccgttc gtcgactgca acgagaagat gcgggtgaac gtgcagaatt acgccgattt
     1621 cggctccatc accgctccct cctgcaccga ctcgcagggc aatctggttc cgccggatgg
     1681 cacaaccttt aggaccggcg gatcgagcga ggtcgtgctt gtcaccgtct gctacgaatg
     1741 ggatctcgcc ggcgtcctgc cgtttctgaa gctcggcaac atgccgaacg gcgctgccct
     1801 gatccaggct tccgcaacct tcctgacgga gccgtacgag gactgaggca tgcgtcgctc
     1861 aggtcggtcg agaaatcgta gtcgcgccgc agccacgttc acgcggatgc tgggcgaccg
     1921 gcggggcatc gcggccgtcg aatttgcgct tattctgccg atcatgttca tgctgctggt
     1981 cggcaccgtc gaattcagcc tcgcgctgac ggtcgaccgc cgggtgagcc aggtcgccaa
     2041 ctcgacggcc gacctcatcg cacagaagaa aaagatcacg gccagcgaag tcgccgggat
     2101 catgaagatc atcgagcacc tgatgcgccc ctatgatccg agccggctgc gcgtcaccgt
     2161 gctcaacgtg atcgctaata tttctgatgc ctcggatacg accgtgtgct ggtcttacga
     2221 gcacaacggc ggcgcgggca gctatgccgc gggggcggag tacgctctgc cggaaaatat
     2281 cgtcgaggcc ggcggcagcg tcatcgtcgc cgaggtggtc tacgattacg agccgatcat
     2341 cttcaaatac ttcatcaaat cggcgttcac cctcagagat acactttacc tcaagccgcg
     2401 gctgtcgagc tacgtcgagt acaacggcca gccgtgcagt tgaggccgtg tggccgagcg
     2461 gacggcgttg accccgtcag ctcgtgcggg tggccacgta tgtgacagct tgacgcaaga
     2521 cgtccgcctt gcccccaaac cgctggagag cggcagcagc ctcggcttgc aagcggttca
     2581 gttgctcgcg cgccccttcc acgcccaacg ccgagacgta cgtcgccttg ccggcggcgg
     2641 catccttgcc ggtggccttg ccgacgacgg cagccgcgcc ttcgacatcg agcagatcgt
     2701 ctgcgacctg aaatgcggcc ccgagagctt ggccgtagct tgaaagcgca cggcgttcct
     2761 cctcccctgc cgacgcgagt atcgcccccg cttcggcggc aaaggcgatc agggcccccg
     2821 tcttgcgctg ttggatgtcg cgcacgcggt cgaggtcgcc ggcgccctgc cggggcagct
     2881 tctctgcctc gagatcaagc tgctgcccgc cgaccattcc cgcaggacca gcagcgcggg
     2941 ccagggcgct tgccagctcg cagcgcacct ccgggtcggg atgcgctccg cggtctgcca
     3001 gaacttcgaa ggcgaacgtc agcagcgcat cgccggcgag gatggcggtc gcttcatcga
     3061 aggcgacgtg gacggtcggt cgaccgcggc gcatgtcgtc atcgtccatg gccggaagat
     3121 catcatggac cagcgagtag cagtgcacca gctcgaccgc cgcagcggcg tctaccgccc
     3181 cttccggcgc gaggccgaag aggcgcgcac tctcgatgac gagaaatggc cgaaaccgct
     3241 tgccgccgcc cagcaccgcg tagcgcatcg cttcggcaag tcgcgggggc acggtcgagg
     3301 tcagcgcgcc caagagctcc tgaaggcgcg cttcggtcaa ttgggcatca atccggagcc
     3361 gttccgatag agccattgta aggccatcta catcgtcaag gttcattata cttgagcccg
     3421 cattctgctg cagccgggag caacccacgc tataacactt ggtccaagga gccggtccat
     3481 aagtatggct ttgcaggaca aagtgcgtga ggt
//
LOCUS       DASVDU010000115        10886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1356946, whole genome shotgun sequence.
ACCESSION   DASVDU010000115 DASVDU010000000
VERSION     DASVDU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356946"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF226_07265"
     CDS             complement(<1..443)
                     /locus_tag="VF226_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX7073826.1"
                     /translation="MFTPGVHSEVGKLNAVMVCRPGLAHQRLTPGNCHALLFDDVLWV
                     HEAQKDHFDFVLRMQERGIEVLDLHDLLAQVLEDAEGRAFILDRRITPNHVGVGAAVA
                     MRPWLDEMPAKLLAEHLIGGIAVDDLPENGVKRLLGDAFGGTDFV"
     gene            complement(1018..1089)
                     /locus_tag="VF226_07270"
                     /pseudo
     CDS             complement(1018..1089)
                     /locus_tag="VF226_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1701..2099)
                     /locus_tag="VF226_07275"
                     /pseudo
     CDS             complement(1701..2099)
                     /locus_tag="VF226_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            2265..2633
                     /locus_tag="VF226_07280"
     CDS             2265..2633
                     /locus_tag="VF226_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7073827.1"
                     /translation="MIAWDAGREATRAVHDALPILVCAEVVHVPCVNPRATMTGHGAQ
                     PGADIALHLSRHGIHTEVEHMQVHDLAPEEAILARVSDLGIELLVASAYGRSADARVA
                     AGGMTRHLLRSTTVPVLMAH"
     gene            complement(2962..3132)
                     /locus_tag="VF226_07285"
     CDS             complement(2962..3132)
                     /locus_tag="VF226_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073828.1"
                     /translation="MISLITTSKLRAKETAFYEADDADHLFEVRQGILKFCQLLPEGG
                     GRSPGSPIPDSL"
     gene            3309..4040
                     /locus_tag="VF226_07290"
     CDS             3309..4040
                     /locus_tag="VF226_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017272074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7073829.1"
                     /translation="MMDSQKVLVVDDDPRVRDLLHRYLEMEGFRVTPVADGAAMREAI
                     RQTRFDLILLDLVLPGEDGLSLARELRQHSETPIIILTGKGDTIDRVVGLEMGADDYI
                     TKPFHLREVLARIRTVLRRARPAMAGAESVPKSGSDQRIAFDGWELDMARRELHSPGG
                     RPIPITTGEFALLEAFARNANRALSRDQLMDLTKGHEWTPFDRSIDTQVARLRKKLED
                     DPNRPTLIKTVRGVGYILAATITRR"
     gene            complement(4027..5973)
                     /locus_tag="VF226_07295"
     CDS             complement(4027..5973)
                     /locus_tag="VF226_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7073830.1"
                     /translation="MENRVPESETSETILDSIGALVIMLDPAGRILFFNRPCQEVSGY
                     SLDEVKKRHVWDFLLPPEDIEPVKAVFERVKAGDSLGRQTNHWITKEGQRRLIAWSTA
                     AKLGPRSEVQYIIRTGLDLTDQEETETALREREARLQSIIETAPDAIITIDAKGIMES
                     FSPAAERLFGYSAAEAIGQNVNILMPSPYREAHDGYLERYLRTGERRIIGIGRVVDGR
                     RKDGTVFPMELAVDEVRLERRRLFTGFVRDLTARRKIEEELRQSQKMEAVGQLTGGIA
                     HDFNNLLTVIIGNLELMEMRLVRDDDREILKQALEAAELGAQLTDRLLTFARRQALNP
                     KPIDLGEMVLGMSDLLRRSLGESFQISTLLAHGLWMTLADPGMAENALLNLAINARDA
                     MLKGGSLTIETENAELDADDLAGYPGGRPGQYVMLSVTDTGTGMTPEVQQRAFEPFFT
                     TKGTGAGTGLGLSMVYGFVRQSGGHVRLYSELGYGTTVKLYLPRLSEAGSGERQQVQM
                     EACAARGEAILVVEDHPQVRRITVRRMEELGYRVLEADSGQAALDLLEQSPAINLLFT
                     DVVMPGGMTGADLARITRRRWPRVRILFTSGYADPTLISGTEFGHGSLLLRKPYSKME
                     LAHKLREALAGLATEGAAGTSNDA"
     gene            complement(6173..6442)
                     /locus_tag="VF226_07300"
     CDS             complement(6173..6442)
                     /locus_tag="VF226_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073831.1"
                     /translation="MTDSTGYEMTFIREVVNAYGKPCEMLLMTISIRRARSRDRAAAA
                     AIKRFERKCRLQRWDALAHTYRVKEINPGISPGAVLLPPTELEAA"
     gene            6695..9304
                     /gene="mgtA"
                     /locus_tag="VF226_07305"
     CDS             6695..9304
                     /gene="mgtA"
                     /locus_tag="VF226_07305"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX7073832.1"
                     /translation="MTFYSKPPTGHGAYWTREAPRLLAELSSSADGLDEATAARRNTM
                     AGKRPVGQGTVADVMRLFLRQLISPLVLILVFGAGVSAATGDRLNTTIILSIVLGSAI
                     LGFSQEYRASAAVAKLRQRLSLKATILRAGRPTEVEAARVVAGDVLLLSAGSLVPADG
                     VVLEAKDLLVSEATLTGETFPVEKRPGTVPAETPLSGRMNCVFQGTSVRSGTARVLVL
                     DTGAATEYGRIGAQLRILQPETDFAVGLRLFGIMLTQVMLVIVVLVLTVNVALDRPVA
                     DTLLFSVALAVGLSPELLPAIVSVTLSVGARAMARNGVIVRRLEAIENLGSMDLLCTD
                     KTGTLTIGVAALDAAVDPDGKASARVLQLAYLNAVFETGIENPLDAAIVAAGRRAGID
                     TEHWHKIDEIPYDFLRRRLTIVVAGTDDPAAHLIITKGALEPVLDCCATLGSEGIRLD
                     AASREALLRFFRRKGAEGFRVLGLAAKRGPAKGRYHHVDETELTFLGFLLLRDPAKPD
                     AAQTVKNLRQLGVKIKVVTGDNRFVAASLALEVGLDPAALLTGAEIAHLSDEALWHRA
                     RHTDLVAEVDPQQKERIIRVLRRSGHVVGFMGDGVNDAPALHGADIGISVEEAVDVAR
                     ESADIVLLRPDLDVLRRGIEDGRRTFVNTQKYIAITLSANFGNMLSMAVASLALPFLP
                     LLAKQILLNNFLSDFPSVAISTDRVDPEATRHPPRWKIANVRRFMLVFGSVSSLFDVL
                     TFALLRLGFGADEMTFQTGWFVVSLLTELAVVLVLRTHRNIFDSRPGMLLLWSTVAVS
                     LAAILIPYAGPLASLFGFVPMPANLVLSLLGIVGLYLLATEVAKRRFYPDAYSATRAA
                     TPSSKIERQRNAS"
     gene            9527..10348
                     /locus_tag="VF226_07310"
     CDS             9527..10348
                     /locus_tag="VF226_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7073833.1"
                     /translation="MIKTILVLADGSPGCEIVLSAAVDLSRRFTAHLNVLHVRPADAG
                     LMPYIGEGMSAAMVEEIIEAAERNAEERAASARTAFDRLNTAGLSASWHAETGPEADI
                     AAAVGRLSDLIVIGRPDDPGEIPWRTTLDSILFDTGRPVLILPRNRPSDIGSRVAVAW
                     NSSAEAAGAVAAALPFFRSAEQVTILSTGPVSRYASAAGLVTYLARHDVRAATKTFEP
                     GSTPLGNALLEQCRLSQSDLLVMGAYGHSRLREMILGGATREVLAIADLPVLMAH"
     gene            10392..10550
                     /locus_tag="VF226_07315"
     CDS             10392..10550
                     /locus_tag="VF226_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073834.1"
                     /translation="MRQRIPLSLEPARNCSYLADATEWHQNRRIGEHASISEPDDVIR
                     TFKVVSVT"
     gene            complement(10610..>10886)
                     /locus_tag="VF226_07320"
     CDS             complement(10610..>10886)
                     /locus_tag="VF226_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7073835.1"
                     /translation="DPDRRRAEFSVLVRSDVKAQGLGWALVTEIIAYARREGVKMLFG
                     EVLRDNTQMLQMCRELGFSIEPDPDDSGIVTVTIDLDGAPASRGAAT"
BASE COUNT         1974 a         3494 c         3350 g         2068 t
ORIGIN      
        1 atgacgaagt cggtgcctcc gaatgcatcg cctaacaatc tcttcacgcc gttttccgga
       61 agatcgtcga cggcgatgcc gccgatcaga tgctcggcga gcagcttggc cggcatctca
      121 tcgagccagg ggcgcatcgc cactgccgca ccgacgccca catgattcgg cgtgatgcgg
      181 cggtccagga tgaaggcgcg cccctcggca tcctccagaa cctgcgccaa gaggtcgtgc
      241 aggtccagga cctcgatgcc acgctcctgc attctcaaca cgaaatcaaa gtggtccttc
      301 tgcgcctcgt gaacccacag cacgtcgtcg aagagcagcg catggcagtt gcccggcgtc
      361 aggcgttgat gggccaagcc gggtcggcac accatcaccg cattcagttt gccgacctcc
      421 gagtgaaccc ccggcgtgaa cattctcttt tctcctgcga ttgtctcctc atcctgcgcc
      481 aagtagttcg ggaggcatta cgtcacatcg cgcctgttgc gtcgtggtgc cgtcagcgcg
      541 gcgtgcaagc ccatacgcgc cgaacagggt cggcctaggc gtcatctccc gctcatccat
      601 ctctatcgct gtgttgagga cccaccgtgg cccaccggaa ttcgggaatg ccacaggatt
      661 gccttcgcga tgaccaaggt cgaacggaga gcggcaaaat gggagaacgt tacggtaact
      721 tacattgccg tccgggctag gctcacccgg gccgagcggc ttcgtgtgcc ctatctgcgc
      781 ccggatgacc gcgaagacag cgatgcctgt gtcgcggcgc ggattaaccg agcaaagggc
      841 taggcgccag cgaaggcacc ataaactcga acgagcggtt ggtgctgctg accgccacgt
      901 aagcgagccg tcagccgaac ggattgtcgg caaagcggct gtcgctgaag aggccgatta
      961 gtatatgatc gaattccagc cccttcgcct tgtgaacgat cccaacggtt tggtctgacg
     1021 ttggacgttg aagctgttgt agacggccgc gtgaactgag agaaatcgct gggcggatcc
     1081 gggcgacttg tcaagcttgg ctttcttccc gacactgccg gccatcacct cgttcgcgcc
     1141 gcttcgcaac gcttgcggct gagagacgat atcgcttcat cggctgtcct cccgtcccga
     1201 gggtccgatc atccagcggc gagtcaagct gggagcggcg tcccaaccgg tgacccacgc
     1261 tcaagcggtc gggttccgag cgaacctgca acggattgat ccggccgcgc cggcctggga
     1321 cacgacagga agcgcacttc gcatcagcta agcggctgga tcggaagcaa ttaaccccgc
     1381 tgcggccaaa atccgagggt ttggggttaa cgcagatggc agcccggtcc cgtggttgtc
     1441 acgccgctca atatcaggct cgggtcgaaa cacctctaga tcgccgagtg gcgaggtccg
     1501 tgcgagccaa agtagcgaaa gatcatcatg gcgtcgatac tctccaaatg cattcgcgca
     1561 gtcccctagg acaagcgccg cgacggtgcg ctatatcagc cgccgaggac cccatttggc
     1621 gcgttccgcc ttgttaggct ttccgaaaaa acctaactat gatcagccaa ggaggtcgct
     1681 tacttagggt ttgaatcaca ggagccgagg cggtccgtaa cgacgacttg cggacagccg
     1741 tgccggcgca gcagcttgcg catcgctttc agcgctgcct gtttgtctcg tcggctctga
     1801 acgaaaacgt ccgacacctc accctcatca tcgaccgccc gcgacagata catccgccgg
     1861 ccgttcgtcc agacaaacat ctcgtccagg tgccagcggc ctgacggacg cggccgtgac
     1921 gggcggatct gccgcgcatc gagagtgccg aacttcgcga cccggcagcg gactgtctcg
     1981 taggatacgt cgacgccacg cgcggcaaga atatccgcca cgtcgcgcaa gctgagcgcg
     2041 aagcgaacgt aaagccacac agcgtgccgg atgatcagcg gcgggaaccg gtgctgggca
     2101 agaaacggtc attatcttga tcgaccgaac ccgcccgcaa aacgccgacc tgttaggttg
     2161 acgctgacgg ttccgatcgt cgcgacggct cgcagttcct tgcgcgcggc cgccgcggcg
     2221 ccagtggcat tatcggcacg cgacgcgata tcggcgagcg ggtcatgatc gcctgggacg
     2281 caggccgtga agccacacgc gcagtgcatg acgccctgcc gatcctcgtg tgtgcggaag
     2341 tggtgcatgt tccgtgcgtc aatccccggg cgaccatgac cggccacggt gcccagcccg
     2401 gcgccgacat cgcgctgcac ctgagccgac atggtatcca caccgaagtc gagcatatgc
     2461 aggttcatga tctcgcgccg gaagaagcga tcctggcacg cgtctccgat ctcggtatcg
     2521 agttgctcgt cgccagtgct tatggccgca gcgcggatgc gcgagttgct gctggcggga
     2581 tgacacggca tttgctgcgt tcgacaaccg ttcctgtcct gatggcgcac tgacccgcgc
     2641 cgtgtcagtc ctgaagcact tgggggtacc ccacacgacg cctgcgagca tcgtcccaga
     2701 gagagatccg aggcgatgct cctcggcgga ccgcgagagg cgtagcagga aggaggcggt
     2761 cttctccaca gcggacttcc ggccgagcag cggcatttga tcctgcgcgg ccgccagttc
     2821 cgtcgccatc agggaccgga tccgccgagc caggctcggc acgtcatcac agagttcctc
     2881 aagccgcgcg cgaatagcgc gccaccgtca cgtcggtacc cgcctcagct gtataggcat
     2941 cgctgttgca caggttgatc cctacaagct gtccgggata ggcgaacccg gggatctacc
     3001 gccgccctcc ggcaggagct gacagaactt cagaatcccc tgacggacct cgaacaggtg
     3061 gtcggcatca tcggcctcgt agaaggcggt ttctttcgcg cgcagtttgc tggtcgtgat
     3121 cagggagatc acaggatcct gcgccggtac gaccgtcccg ggccagaccg gcgtaagcgc
     3181 gacgttggca aagtgtcacg ggccgatggc agcggcgcca ttttctggta attaagagca
     3241 acggattgct acgttcgtag caaccggtac cgaaccggac ggggttccga cacctgacct
     3301 ggaccgccat gatggacagc cagaaagtcc tcgttgtcga tgatgacccg agagtccgtg
     3361 accttctgca ccggtatctc gaaatggagg gattccgggt cacgccggtc gccgatggcg
     3421 cggcgatgcg cgaggcgatc aggcagacgc ggttcgatct gatcctcctc gacttggtcc
     3481 tgcccggaga ggatggtctc tcccttgccc gggagctgag gcagcattcc gagaccccca
     3541 ttatcatcct gacgggaaaa ggcgatacga tcgaccgcgt cgtcgggctc gagatgggcg
     3601 ccgacgacta catcaccaag ccgttccatc tgcgcgaggt gcttgcgcgt atcaggaccg
     3661 tgcttcgccg cgctcgccct gccatggccg gcgcggagag tgtgccgaag agcggcagcg
     3721 accagcggat cgcgttcgac ggctgggagt tggacatggc ccggcgcgag ttgcactcgc
     3781 ccgggggaag accgattccg attacgaccg gcgagttcgc gctcctggag gcgtttgccc
     3841 gaaacgccaa tcgggcgctc agccgcgatc agctgatgga tcttaccaag ggccacgagt
     3901 ggaccccctt cgaccgcagc atcgatactc aggttgcccg gcttcgaaag aagctcgagg
     3961 acgatcccaa cagaccgaca ctgatcaaga ccgtgcgtgg tgtaggttat atccttgccg
     4021 cgacgatcac gcgtcgttag acgtgcctgc ggccccctcg gtagccagtc ccgccagagc
     4081 ctcgcgcaac ttgtgagcca gttccatctt cgaatagggc tttcgcagga gcagcgatcc
     4141 atggccgaat tccgtaccgc tgatgagcgt gggatcggca taacctgacg tgaacaggat
     4201 ccggacccgg ggccagcgac gacgggtgat ccgggccaag tcggcccctg tcataccgcc
     4261 cggcatcacg acgtcggtga aaagaagatt gatcgctgga ctctgttcca gcagatcgag
     4321 tgcggcctgt ccactgtcgg cctcgagtac gcggtaaccg agttcttcca tccggcgaac
     4381 cgtaattcgc cgcacctgcg ggtgatcctc gacgaccagg atcgcttcgc cgcgcgcggc
     4441 acacgcctcc atctggacct gctgccgctc gccgctccct gcctcgctca agcgcggcaa
     4501 gtaaagcttt accgtcgtcc cgtagccgag ttcgctgtaa aggcggacat gtccacccga
     4561 ctgacgtaca aagccataga ccatgctgag gcccagaccg gtgccggcac ccgttccttt
     4621 tgtcgtgaag aaaggttcga acgcccgttg ctggacttcg ggagtcatgc cggtgccggt
     4681 atcggtgacg gagagcatca cgtactgtcc cggtctgccc cccgggtaac ctgccaagtc
     4741 gtcggcgtcc agttccgcat tctctgtctc aatggtcaga ctaccgccct tcagcatggc
     4801 atcacgcgca ttgatggcga gattgagcaa cgcgttttcg gccatgcccg gatcggcaag
     4861 agtcatccag aggccgtgcg cgaggagcgt gctgatctga aacgactctc ccaaggaacg
     4921 ccgcaaaaga tcgctcatgc ccaaaaccat ctcgcccaga tcgatcggct tgggattgag
     4981 tgcctggcgc cgggcaaagg tgagcagcct gtcggtcagt tgcgcgccca gttcggcggc
     5041 ctccagcgcc tgcttcagga tctcgcgatc atcatctcga acgagccgca tctccatcaa
     5101 ttcgagattg ccgatgatca ccgtcagaag attgttgaaa tcgtgcgcga tcccgccggt
     5161 cagctggccg accgcttcca ttttctgcga ctgccgcagt tcctcctcga tcttgcgccg
     5221 ggcggtcaaa tcacgaacga atccggtgaa cagccgtcgc ctttcgagac ggacctcgtc
     5281 gacggccagc tccatgggaa aaacggttcc gtccttccgc cggccatcga ccacgcggcc
     5341 gattccgatg atccttcgct cacccgtcct gagatagcgc tccagatatc cgtcatgcgc
     5401 ctctcggtag ggcgacggca tcagtatatt gacgttctgg ccgatcgcct cggcggcgct
     5461 gtacccgaac aatcgttcgg ccgcgggact gaaactctcc atgatcccct ttgcgtcaat
     5521 ggtgatgatc gcatccgggg cggtttcgat gatcgattgc agtcgcgcct cccgctcgcg
     5581 caacgcggtc tcggtttcct cctgatcggt gaggtcgagc ccggtacgga tgatgtactg
     5641 cacctcgctc cgggggccga gtttcgccgc cgtcgaccag gctatgagac gccgctggcc
     5701 ttccttcgtg atccaatggt tggtttggcg gccgagagaa tcgcctgctt tgactcgctc
     5761 gaacacggct ttcacgggct cgatgtcctc cggtggaagc aggaagtccc agacgtgcct
     5821 cttcttcacc tcatcgagcg agtagccgct cacctcctga cagggcctgt tgaagaacag
     5881 aatccgcccg gccggatcga gcatgattac gagggcaccg atcgagtcga gaatcgtttc
     5941 ggaggtctcg ctctctggca cgcgattctc catgtcgaac tccggctcgc acattgcgtc
     6001 gttatgccgg gtgcattgct gccgtgacga acgggagcgt actcgtccgc ccttcggctg
     6061 taaattcgga tccttgacag ccgcgagcgg tacgcctcgc taaaggtgaa tcgagaccgt
     6121 agcagaatgc ggaaccgagc gagcctcctc ggagacggcc caaccgccgc cactatgccg
     6181 cttcgagctc ggttggcgga agcaataccg ctcccggcga tatcccggga ttgatttcct
     6241 tgacccgata tgtatgggcc agcgcatccc atctctggag acgacacttc cgctcgaagc
     6301 gcttgatcgc cgcagccgct gcacggtcgc ggctacgggc tctgcgaatg gaaatcgtca
     6361 tcagcagcat ctcacatggc ttcccatacg cgttcaccac ctcacggatg aatgtcatct
     6421 cataacccgt gctatcggtc atagccgatt cctccgtcag tcctcatcct tcagatctac
     6481 gttatcggaa tcgtctcgcg tctcattgat cggaatcaaa cgagtctcca ggcccgcctt
     6541 ggcttgagga tcgagcatgc atgcggtgga tacccgttcg tggctttccg tctctgaccg
     6601 gcgaaaggaa agccggccgg cctctgcgct agatcaatgt gggcgcatcc ccgagcgttt
     6661 agctttggcg cgcagcacct gtcggcatac cggcatgaca ttctattcga aacctccgac
     6721 cggacacggc gcctactgga cgcgcgaagc acctcgtctg ctggcggagc tgagcagttc
     6781 cgccgacgga ttggacgagg ctacggcggc acgccggaac actatggcag gcaagcgtcc
     6841 cgtcggccag gggaccgttg cggacgtgat gcggctgttc ctcaggcagt tgatcagccc
     6901 gctcgtcctg atcctggtgt tcggtgccgg cgtgtccgcg gccacgggcg atcggctcaa
     6961 taccactatt attctttcca tcgttctggg cagcgcgatc ctgggcttct cgcaggaata
     7021 ccgggcgtcg gcggccgtgg caaagctgcg gcagcggctt tccctcaagg cgaccattct
     7081 gcgcgccggc cgaccgaccg aggtcgaggc tgcgcgcgtg gttgcggggg acgtgctgct
     7141 gctatccgcc ggaagcctcg tcccggctga cggtgtcgtg ctggaggcaa aagaccttct
     7201 cgtttccgag gcaaccctga cgggcgaaac ctttccggtg gagaaacggc caggcacggt
     7261 tccggcggaa acgcccttgt cggggagaat gaactgcgtt ttccagggca cctccgttcg
     7321 gtccggcaca gcacgggttc tcgtgctcga cacgggcgcc gcgaccgagt atgggcgcat
     7381 cggcgctcag ctgcggatcc ttcagccgga gaccgacttt gccgtaggcc tgcgtctttt
     7441 tggcatcatg ctgacccagg taatgctggt gatcgtggtc ctggtgctca cggtcaatgt
     7501 ggcactcgat cgtcctgttg ccgacacgct gctcttctcg gtcgcgctcg ccgtcggcct
     7561 gtcacccgaa ctgctgccgg ccatcgtcag tgtgacgctc tcggttggcg cacgggccat
     7621 ggctcgaaac ggagtgatcg tccgccggtt ggaggcgatc gagaacctgg gcagcatgga
     7681 tctcctctgc accgacaaga ccggaacgct gacgatcggc gtcgccgccc tggacgcggc
     7741 ggtagatccc gacggaaagg cgtcggcgag ggtgctgcaa ctcgcttacc tgaatgcggt
     7801 gttcgaaact gggatcgaga acccgctgga cgcagccatc gtcgcagccg gacggcgggc
     7861 cgggatcgac accgaacatt ggcacaaaat cgatgagatc ccgtatgact ttctacgccg
     7921 ccgcctgacc atcgtggtcg ccggcaccga cgatccggca gcccatctga tcatcaccaa
     7981 gggggcgcta gagccggtcc tggactgctg cgccaccctc ggctcggagg gtatccgcct
     8041 ggacgccgcc tcccgcgagg cgttgctgag attcttccgc cgtaaggggg ccgagggctt
     8101 ccgtgtgctg ggccttgcgg ccaagcgggg accggcgaag ggtcgctacc accacgttga
     8161 cgagacggag ctgaccttcc tgggcttcct gctcctgcgc gacccggcca agccggatgc
     8221 ggcacagacg gtgaagaacc tgcggcagct gggggtgaag atcaaggtag tgacgggcga
     8281 caaccggttc gtcgcagcaa gtctcgctct cgaggtgggg ctcgaccccg ccgccctcct
     8341 gacgggggcg gagatcgccc acctgtccga cgaggccctc tggcaccggg cccggcacac
     8401 cgatctcgtt gccgaggtcg atccgcagca gaaggagcgg atcatccgcg tgttgcggcg
     8461 gtcggggcat gtcgtgggat tcatggggga cggcgtaaat gatgcgccag cccttcatgg
     8521 cgccgatatc ggcatttcgg tggaggaggc tgtcgatgtt gcccgggaaa gcgccgatat
     8581 cgtgctgctg cggccggatt tggacgtcct gcgccgaggg atcgaggatg ggcgtcgtac
     8641 ttttgtcaac acgcagaaat atattgctat tactctcagc gccaacttcg gaaacatgct
     8701 gagcatggcc gtagcctcgc tcgcgctccc cttcctgccg ctgctggcga agcagatcct
     8761 gctcaacaac ttcctttccg acttcccgtc cgtggcgatc tccacggacc gggtggaccc
     8821 ggaggccacg cggcatccgc cgcgctggaa gatcgcgaat gtccgccgct ttatgctggt
     8881 gttcggatcg gtcagcagcc tgttcgacgt tctgacgttc gcgctgctcc gcctgggatt
     8941 cggcgcagac gagatgacgt ttcagaccgg atggttcgtt gtctccctgc tgacggagct
     9001 cgccgtcgtc ctggtgctcc gcacgcacag gaacatcttc gacagccggc ccggaatgct
     9061 cctgctctgg tcaaccgtgg ctgtcagcct cgccgccatc ctgatcccct atgccggacc
     9121 gttagcctcc cttttcggct tcgtgccgat gccggcaaac ctggtcctgt ctctgctcgg
     9181 gatcgtcgga ctatatctgt tggccacaga ggttgcgaaa cgccggttct acccggacgc
     9241 ctatagcgcg acccgcgccg ctacgccgag ttccaagata gagcgtcagc ggaacgcttc
     9301 gtgacggttt gtggcagacg gtgggcatgc cgaagcaaag gcccggccgc atcgcggatc
     9361 gtcagccgac tcggaggcaa aaaggcccga caccattctg gcggggtcgt catacgccat
     9421 cacgcggaac ttgatccagg tcaaggcctt tgccgcccgc gaggcgccta atgccaagta
     9481 atcccggccg ttgaatcatc gtcgagaaag ccgacaggag ctcgccatga tcaagacgat
     9541 cctcgttctc gctgacggaa gccctggatg cgagatagtg ctatccgccg cggtcgacct
     9601 cagccgacgc tttaccgctc atctgaacgt cctgcacgtc cgccccgctg acgcaggcct
     9661 gatgccgtat ataggcgagg ggatgtcggc cgccatggtc gaggagatca tcgaggcggc
     9721 ggagaggaac gcagaggaac gggcagccag cgcaaggacc gccttcgacc ggctcaacac
     9781 ggccgggctt tcggcaagct ggcatgccga aaccggcccg gaagcggata ttgcggcggc
     9841 cgtcggacgg ttaagcgatc tcattgtcat cggacgcccg gacgatccgg gtgagatccc
     9901 atggcggaca acgctcgatt ccattctatt cgataccggc cgaccggtct tgattcttcc
     9961 ccggaatcgg ccatcggaca tcggcagccg cgtggccgtc gcctggaaca gcagcgccga
    10021 ggcggccggc gcggttgccg ctgcccttcc ctttttccga tccgccgagc aggtcacgat
    10081 cctgagcacc ggccctgtca gtcgctatgc ctcggcagcc gggctcgtca cctatctcgc
    10141 ccggcacgac gttcgggccg cgacaaagac gttcgagccg ggctcgacac cgctcgggaa
    10201 cgcattgctg gagcagtgcc ggctgtctca atcggatctg ttggtgatgg gggcctacgg
    10261 ccatagccgc cttcgcgaga tgattctcgg aggtgccacc cgcgaagttc tggccatcgc
    10321 tgacttgccg gtgctgatgg cacattgatc ggccgtcgca ccccggtggc gatctccttg
    10381 acgtgagcct cgtgcgccaa cgaatcccat tgtcgctgga accggcgcgg aattgcagtt
    10441 atctggccga tgcgacggaa tggcaccaga accgccgcat cggcgagcat gcatccatca
    10501 gcgagccgga tgacgtgatc cgtaccttca aggttgtttc cgtgacatga cggttgccgg
    10561 ccgcagggcg gtatgccctg caaaaaacct tctgcgtccc tggtatggct cacgtggccg
    10621 cgcctcggct cgccggtgcc ccgtcgagat cgatggtcac ggtgacgatg ccggaatcgt
    10681 cgggatcggg ctcgatcgag aacccgagtt cgcggcacat ctgcagcatc tgggtattgt
    10741 cgcggagaac ctctccgaac agcatcttga ccccctcccg tcgggcatag gcgatgatct
    10801 cggtcacaag cgcccagccc agcccctgcg ccttgacatc gctgcggacg aggacgctga
    10861 attccgcgcg ccgtctgtcg ggatcg
//
LOCUS       DASVDU010000116        18455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1140819, whole genome shotgun sequence.
ACCESSION   DASVDU010000116 DASVDU010000000
VERSION     DASVDU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140819"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..907
                     /locus_tag="VF226_07325"
     CDS             80..907
                     /locus_tag="VF226_07325"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7073836.1"
                     /translation="MPTGDVVIDLEELVARIPDGASIAIPPDYSGCAMAAVKALIRRG
                     CRNLNLIGVPSGGFQADWLIGAGCVASVEAAAMTLGEHGIPPRFSAAIREGRIAMKDA
                     TCPAIHAGLQAAEKGVPFLPLRGILGSDLMKYRPDWKVVDNPFSDGNDPIVLIPAIRP
                     DIALFHARAADRHGNVWIGVRRELMLMAHAAQTTLVTVERIEDTDFLADDAMAAGTIP
                     ALYISAISVVPGGARPVGLAGAYPADTEMLSAYAAAAKTEDGFRRWAGEAIFGKVPA"
     gene            904..1653
                     /locus_tag="VF226_07330"
     CDS             904..1653
                     /locus_tag="VF226_07330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7073837.1"
                     /translation="MSPHLSDAVSRTEIMIAAITRMLQGLRHVAVGNSSPIPGSAALL
                     ARALSGGKLRVSLLGSRTHNAFTDGGRELFDCAAQGRIDAFFLSGGQIDGAANINLVG
                     LGGYPQSKVRFPGSFGSAYLYHLIPRVILFREEHTPRTLVPKVDFISAAGTSPENEYR
                     PGGPYALVTGLCVFSFDRQRRQFRLQSVHPGVSAADVREATGFDYAAPENVPVTEPLD
                     DGALELLRRDIALQVAETYPAFAAKTWGVGV"
     gene            1650..2462
                     /locus_tag="VF226_07335"
     CDS             1650..2462
                     /locus_tag="VF226_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX7073838.1"
                     /translation="MTIGRNRREDEMLTLKNPARERLQNGELAIGIGLRQARTVDAAL
                     ALKTCGFDWLFIDLEHGAMGLDTAVQISVAALVAGISPLVRVPRGRYDLAARALDGGA
                     YGIVMPHVDTAEEAREIVSHLKFPPAGHRSITGAMPQLAFRSAPLAEAAAAVNENVLI
                     VAMLETPEAIANADAIAAVPGIDVLLIGTNDLTCEMGIPGELMHERVGAAYETMVAAC
                     RKRGKWAGMGGVYSEEGLRRYIELGAQMVLAGSDLSFLMAAGQARTKMLRGI"
     gene            complement(2779..3333)
                     /locus_tag="VF226_07340"
     CDS             complement(2779..3333)
                     /locus_tag="VF226_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7073839.1"
                     /translation="MQRIQCQGVHHITLVGANRQTSIDFWEGLLGMPLVFEQPNLDAP
                     DQNHLYFDPGDGRLITIFTNESWQPDPRPNPTDVGNVHHIAFTVSRATYTQAAARLRE
                     RGFANSGEVDRGFMYSLYFRDPLGQLLELACYKFEPPAGYTHADVLREAHKIRLAKGA
                     YNITDEHLADAIETLSRPREPASV"
     gene            complement(3373..3588)
                     /locus_tag="VF226_07345"
     CDS             complement(3373..3588)
                     /locus_tag="VF226_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073840.1"
                     /translation="MAFEDVQAELGLLLTQMENETQPDDRHEIYLVIKRKLNELKAYG
                     MPLPQDLVELERSLEQEFEADQRGEGA"
     gene            complement(3683..4885)
                     /gene="pcaF"
                     /locus_tag="VF226_07350"
     CDS             complement(3683..4885)
                     /gene="pcaF"
                     /locus_tag="VF226_07350"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002968962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX7073841.1"
                     /translation="MRPAYLVSGIRTPIGRYGGSLSSVRPDDLAAHVVRELMARLPSV
                     PATAIDDVIFGCANQAGEDNRNVARMSALLAGLPAEVTGTTVNRLCGSGLDALAQAAR
                     MIRTGEAEVVIAGGVESMSRAPFVMGKATEAFSRQAEIFDTTIGWRFINPLMKEKYGT
                     DSMPETAENVAEEFQISREDQDAFALRSQLRAAAAQKNGRLDKEIAAVAVPQRKGDPI
                     VVAKDEHPRETTLEKLAKLPTPFRKGGTVTAGNASGVNDGAAALLVASEAALEKYGLK
                     PLARVVGAATAGVPPRIMGIGPAPATRKLMERLALKISDFDIIELNEAFAAQALAVTR
                     QLGLPDDAEHVNPNGGAIALGHPLGMSGARLALTAALELKERNARRALCTMCIGVGQG
                     ISLVLEAA"
     gene            complement(5019..5657)
                     /locus_tag="VF226_07355"
     CDS             complement(5019..5657)
                     /locus_tag="VF226_07355"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEX7073842.1"
                     /translation="MDLDNAISECPLIAILRGITPAECEAVGTALVETGFRIIEVPLN
                     SPEPLDSIRILADRFGAAALIGAGTVLSPAAVDDVAGAGGRLIVMPHSDPAVITAAKS
                     QGLACLPGVATPTEAFAALAAGANALKLFPAEAIPPSAVKALRAVLPPGTRLLPVGSI
                     GAHNMAAYLKAGAAGFGIGSTLYRPGKTVDDVRREAKALVEAYASAARPTAL"
     gene            complement(5644..6576)
                     /locus_tag="VF226_07360"
     CDS             complement(5644..6576)
                     /locus_tag="VF226_07360"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX7073843.1"
                     /translation="MSKPHLIAVDWGTTNFRAYLADKNGTILAQTESPRGILAVQNAA
                     FADVMRAEIAGWISAHGPLPVMMSGMIGSRQGWVEAPYVRCPAGIRDIAAALTTFEVD
                     GIGTVHLVPGLDHMPQGEPPDVMRGEEAQIIGAMALLSTQNATFVHPGTHSKWVTIAD
                     GIITGFATYMTGEVFGALKNHTILGRLMKDGDHRRAGFLQGVEAARRGTSGGGLLHQL
                     FSVRTLGLFEMLPAEELSGYLSGLLIGSEVMAAAKPGQSITIFGSAELTDRYGKAAEA
                     LGFAWHRGPDHSVVAGHLLLARAAGLLEVSHGPR"
     gene            complement(6632..7609)
                     /locus_tag="VF226_07365"
     CDS             complement(6632..7609)
                     /locus_tag="VF226_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7073844.1"
                     /translation="MRAAIRTKGQSWIGVLCGLAAAAIWGGGAVVSRHLVTTSLDPIS
                     LVFLRYVGCFPIALILVAPFRDRVRLTIGWPRLVALLLLGGPLYHGLLVAGYQHATAG
                     GGALLLSGLLPVFALGLEYLHTGALPRAGLIGSAAVLAGLALFSFGSEILDLSFSAFL
                     IFAVAAAAWALLNSCVKCWRVDPIGLAIALALLAPIFLPIYLIGSPQSELPATAGEIA
                     LQIVYHGWLVAIGATALFFTCVRLAGAHIAAVLQVMSPGFSVLFGVAFLGEAVVPAQG
                     IGLMLVFGGVLLTMSSPAHQAPVGERALQTIIPRSIPPGLAARTRWSRW"
     gene            complement(7676..8407)
                     /locus_tag="VF226_07370"
     CDS             complement(7676..8407)
                     /locus_tag="VF226_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073845.1"
                     /translation="MSSIGRRLAQKLKESRSTETPALESVNWQVRTGLSVPFGSHMAG
                     GGLASAPSTEPVNFQVRTGLSVPFASFMKSQPSTDHPVAEPVNFQVRTGLSVPFNPYM
                     LGAHAEELLKKRVMISPVVVSWRYSVTSPEDFTDWLTTREILLSESRMAGDPEIKGVR
                     YGGTYRIASENDEANARFKTIWGYTSEAAMHTMHRLCSDESVSATLVQLELIDFVKGL
                     KRFIAEAGDEHFSEEVMISAAAAAA"
     gene            complement(8494..8781)
                     /locus_tag="VF226_07375"
     CDS             complement(8494..8781)
                     /locus_tag="VF226_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX7073846.1"
                     /translation="MFSRNGCYTKTENVVLRRFEEWGRCYAFTPDEPEVYDLNTSAWF
                     ILELCDGRPFQQIEADYVATLGPRVGRDKAKAQFHAGFDELLSRNIISAIE"
     gene            complement(8747..9790)
                     /locus_tag="VF226_07380"
     CDS             complement(8747..9790)
                     /locus_tag="VF226_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7073847.1"
                     /translation="MNELVRFDGAHEPRRVPRLEKPFKLTICVTQTCSMNCKLCYADC
                     GSSKRPELTTEQWKTFIDQLVAEEFIHIFFEGGEPFHRPDFEDILAHCNRRLYVAVRT
                     HATLIDKARADRLKKLGVGRLYVDLFAAIPEVQDELTGQSGSYDATIAGIRNARAAGI
                     KVTILGILSRKNHEHLQRYLDLAAELGCDQVGVLRLYPLGRAKRNWSELSLSLDEMMR
                     ALHGLRIPPGVQLMQSWHPRDGNCCWQNAAVSPQGDSIGCPYLREYVNYGNILDTPFV
                     ETWNHPLYRELRSNSVEGSCPECSATQGTFGGCRATAYAFHGRWTAPDPYCVHTNEGV
                     DLRVLPQRLLHEN"
     gene            complement(9787..11370)
                     /locus_tag="VF226_07385"
     CDS             complement(9787..11370)
                     /locus_tag="VF226_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7073848.1"
                     /translation="MANRAQPFNASALQQMGVPPLDSGIDGRGIVIGVIDYGFDILHP
                     CFRYTATGQTRFRYLWDQNTGREFDARDVDRLIAASDRTGSRDAADAEYDPHANYFGR
                     YGVQSGAHGTVMAAIAAASPVAGFRGVAPAADLIAVQLGLLDHHWREEDENGLPTWRH
                     WRPEMEPVWNGWRSYDEAAQIVSALEYIYARAVHLNARGLVVNLSIGAYAGAHDGRSR
                     VEQAISDLTGRCLQSDLPCAVVVGAGNAGLEEGHFGGKAEPGRPLSFAWRMHRDDPTQ
                     NKLEIWYRSETPLEISVSPHPGHPGWSISPGRTHTIELCGTRIGIADHVPFARSPRSR
                     IRIAVHPPYVPDHFWHEYSGELSLEINCSATGSCPADIHAWVERDDGLANRSTLYPAY
                     AESTLASLAAAEGAIIAAGYDHHRTAANPDPFPASSLGPLPWAVTKDAANLICAPAHR
                     IWGARSKTAGFVETSGTSAAAALTSGAMALLMQCLASRRGLEPQRWTDLLVPRRDTWS
                     PRFGFGPVNIADTLKEVRG"
     gene            11786..12556
                     /locus_tag="VF226_07390"
     CDS             11786..12556
                     /locus_tag="VF226_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006108271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7073849.1"
                     /translation="MSVVLAEHPAEGVLLLRINRPEARNALNLEVRRALVAELKAAAE
                     DQQIRAAVLTGNDKAFAAGADLREMRDLGPIEAMQRGTHKLWDEIAAFPKPLIAAVNG
                     FALGGGCELALHCDIIIAGEGAKFGQPEVKVGILPGGSGTQRLVRAVGKYKAMLMILT
                     GEIIGAREASEMGLVSRVVADDQVVSHAIEMAANIAKLAPLAVELAKEAALTGQDASL
                     ATGLSLERKALWLAFATEDKREGMTAFLEKRAPNFKGK"
     gene            12560..14092
                     /locus_tag="VF226_07395"
     CDS             12560..14092
                     /locus_tag="VF226_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX7073850.1"
                     /translation="MALDPNREDLVVGVIGTGAMGRGIMQVAAQGGMQVFAYDEKSGA
                     AESAVQHIRQMLDGLVAKGRLDKAGADAAVARIVIADGLAQIATADVIIEAIVEQLGI
                     KQELFATLDELAGPDTIIASNTSSLPITAIAARCKRPERVAGMHFFNPVPLMKLVEVI
                     PGLKTDPQVIEALTTVARRMTRVPVVCTDSPGFIVNHIGRAYVPEAARIVAEGIAEPA
                     EIDRIMTEAVGMRMGPFTLLDLVGADVSVSVMESLWSQFYSEPMYAPQPILKLRVAGG
                     LYGRKTGAGWYTYEDGKRVEPPRPEPPAARPRSVWVSPRHPAASAKAIALLQRLGANI
                     ENGNAPSAEALSIVTPTSGDCSSEIVQQGLDRTRTVALDTFFHFEKHRTLMVPPGIDP
                     SMREAAHGLLASDGAGVSVINDSPGFVAPRIVAHIINVGCQVAQQKIASPSDIDLGAK
                     LGLSYPYGPLEWGDHLGPDWVLEMVAELERFYEEPRYRPSPWLKRRALLGLSLTTPDG
                     RH"
     gene            14149..15225
                     /locus_tag="VF226_07400"
     CDS             14149..15225
                     /locus_tag="VF226_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX7073851.1"
                     /translation="MNKDSWQRLSAAMPMLWTLLLTLCVGAAGGAVFFLLGLPAAWLS
                     GPAVSVAGASLAGFRLSVPNLARQAAFVFLGSTMGATMTPETLALMARWPVSLAGLAF
                     CLLGIMVCCSAYLQHVHGYDRTTARLAAVPGALPYVLALAAESRGDQYRIAIIQIFRL
                     AALLIFLPNVLSLFGYEAESLAPKTSTPVNLLEVALMLVGGVAGAVVFAWIKAPAASL
                     FGSMVAAGLLYASGTLSSPVPGWLMLPGFLVIGATVGTNFTGIDRRLILETLLASIGS
                     LLVGTAIALAFAVPVSWLLGLTVAQLWLAYAPGGVDVMTIMAMALGLDPAFVGAHHVA
                     RFIGLGFFVPVWMRAGLSQEERRG"
     gene            complement(15263..16462)
                     /gene="sucC"
                     /locus_tag="VF226_07405"
     CDS             complement(15263..16462)
                     /gene="sucC"
                     /locus_tag="VF226_07405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7073852.1"
                     /translation="MNIHEYQAKELFRQYGVPTPTGHPAFTVEEAVAAAEKLPGPVWV
                     VKAQIHAGGRGKGKFKEAAAGDKGGVRLARSIDEVRTFAQQMLGNTLVTVQTGPAGRV
                     VKRLYVEDGSQIERELYLSALVDRTTSRIAFIASQAGGMDIEQVAHDTPEQIVTLSID
                     PASGYFPFHGRKIAFALGLSGDQVSQCVKMVGDLYRLFIEKDMSLLEVNPLIVTKDGK
                     LLCLDAKMNFDSNALYRHKEIVELRDLDEEDPMEVEASKYDLSYVKLDGTIGCMVNGA
                     GLAMATMDIIKLYGEEPANFLDVGGGASKEKVQAAFKIILSDPAVKGILVNIFGGIMR
                     CDIIAEGVVAAAREMAINVPLVVRLEGTNVDLGKKILAESGLKIVPADNLADAAQKIT
                     AEVKKAA"
     gene            complement(16663..17046)
                     /locus_tag="VF226_07410"
     CDS             complement(16663..17046)
                     /locus_tag="VF226_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX7073853.1"
                     /translation="MSKKAIEAAPLRAVGPYSLAIDTGQLVYLSGQVHIDGETGKLVE
                     GDIGTQTRQCLENLKKVLASAGLGFEHVVKTTVFLVDMADFAAMNQVYAEYMVAPFPA
                     RSTIQVAALPLGARVEIEAIAQRNT"
     gene            complement(17098..17523)
                     /locus_tag="VF226_07415"
     CDS             complement(17098..17523)
                     /locus_tag="VF226_07415"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003566844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7073854.1"
                     /translation="MKRTASAEWKGGLKDGKGTISTQSKLLTNAPYSFGTRFNQDAGT
                     NPEELIAAAHAGCFSMALSLQLGNAGLTPESIRTEAAVTLEKVDSGFAITAVHLDVVA
                     KVPGADQAAFEKAANEAKEGCPVSKVLNAKITMDAKLET"
     gene            complement(17568..>18455)
                     /gene="mdh"
                     /locus_tag="VF226_07420"
     CDS             complement(17568..>18455)
                     /gene="mdh"
                     /locus_tag="VF226_07420"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7073855.1"
                     /translation="LGDVVLFDIMEGIPQGKALDIAQAGGVEDFDCVLKGTNSYADIV
                     GAQVCIVTAGVPRKPGMSRDDLLEINLKVMQQVGEGIKQYAPDAFVIVITNPLDAMVW
                     ALQKASGLPHSHVVGMAGVLDSTRFRHFLAEELKVSVEDVTAFVLGGHGDDMVPLVRY
                     STVAGIPLPDLVKMRWMSQEKLDQIVERTRKGGGEIVALLKSGSAYYAPASSAILMAE
                     SFLKDKKRVLPCAAFLNGEYGVRGLYVGVPVVIGTGGVERIVEIELNDEEKAMFSKSV
                     DSVKSLVEACTRIAPQLVA"
BASE COUNT         3266 a         5738 c         5934 g         3517 t
ORIGIN      
        1 tgcaagtacg gcgtatccgc tgcttgtgtc ggaggatcgc cgggtccaaa gtgcgccgcg
       61 atttggaggg agcatggcca tgcccacagg tgatgtggtg atcgatctgg aagagctggt
      121 tgcacgcatt cccgacggcg ccagcattgc cattccgccg gattattcgg gatgcgccat
      181 ggcggcggtg aaagcgctca ttcggcgcgg ctgccgcaac ctgaacctca ttggagtccc
      241 gagcggtggc ttccaggccg actggctgat tggcgccggc tgcgtcgcga gcgtcgaggc
      301 ggcggcgatg acgctcgggg agcacggcat tccgccacgc ttctcggcgg cgatccgcga
      361 agggcggatc gcgatgaagg atgcgacctg cccggcgatc cacgcagggc tgcaggcggc
      421 cgagaaaggt gtccctttcc tcccgctccg cggcatcttg ggctcggatc tcatgaaata
      481 ccggccggat tggaaggtgg tggacaatcc tttctcggat ggaaatgatc ccatcgtgct
      541 cattccggca attcgccccg atatcgcgct gttccacgcc cgggccgcgg atcgccacgg
      601 caatgtctgg atcggcgtgc gtcgcgagct gatgctgatg gcccatgccg cgcaaaccac
      661 gctggttacc gtcgagcgca tcgaggatac cgacttcctc gccgacgatg ccatggcggc
      721 ggggacgatt ccggcgcttt acatttcggc gatttctgtg gtgcccggtg gtgcgcgacc
      781 tgtcgggctc gccggtgcgt atcccgccga tacagaaatg ctttctgcat acgcggcagc
      841 agcgaaaacg gaggacgggt ttcggcgctg ggcgggcgaa gccatttttg gaaaggtgcc
      901 tgcatgagcc cgcatttgtc cgacgcggtc agccgtacgg agatcatgat cgccgcgatc
      961 acgcggatgc tgcaggggct gcgccatgtc gcggtcggca acagctcgcc cattccggga
     1021 tcggcggccc ttctcgcgcg ggcgctctcc ggcggaaagc ttcgcgtgtc gctgctcgga
     1081 agccgcacgc acaatgcctt cacggacggc ggacgcgagc tgttcgattg cgccgctcag
     1141 gggcggatcg atgcgttttt tctctccggc ggtcagatcg acggcgcggc caatatcaat
     1201 cttgtcggtc tcggcggtta tccgcagagc aaagtgagat tcccaggctc gttcggctcg
     1261 gcttatctct atcacctcat tccgcgggtg attttattcc gtgaggagca cacgccgcgc
     1321 acgctggtgc ccaaggtcga cttcatcagc gccgccggaa cctcgcctga aaacgaatat
     1381 cgtccgggcg ggccgtatgc gttggttacg gggctgtgcg tgttttcatt cgacaggcaa
     1441 cgccgtcaat tcaggctgca aagtgtccat cccggtgtca gtgctgccga tgtcagagag
     1501 gccaccggat ttgattatgc cgccccggaa aacgtgcccg tgacagagcc gttggacgac
     1561 ggagcactgg agctattgag acgcgatatc gcccttcagg tcgccgagac ctatccagcg
     1621 ttcgctgcga agacgtgggg cgtcggggta tgacgatcgg caggaacaga cgcgaggacg
     1681 agatgctcac cctgaagaac ccggcacgcg aacggctcca gaatggagag ctcgccatcg
     1741 gcatcggcct ccgccaagcc cgcaccgtgg atgccgcgct ggcgttgaag acctgcgggt
     1801 tcgactggct gttcatcgat ctcgagcacg gggccatggg gctcgacacg gccgtgcaga
     1861 tttcggtcgc cgcgctggta gccggcatct ccccgctggt tcgtgtgccg aggggccgtt
     1921 atgaccttgc ggcgagggcg ctcgatggtg gtgcgtacgg aatcgtcatg ccgcacgtcg
     1981 acaccgcgga ggaggcgcgc gagatcgtat cgcatctaaa atttccgccg gccggccacc
     2041 gctcgatcac gggcgccatg ccgcaactcg cgttccgatc ggcaccattg gccgaagccg
     2101 cggcagcagt gaatgaaaac gtgctgatcg tggcgatgct cgaaacgccg gaggcgatcg
     2161 ccaatgcgga cgcgattgcg gccgtaccgg gcatcgatgt gctgctcatc ggcaccaacg
     2221 acctcacctg cgagatgggc attcccggcg agctgatgca cgagcgcgtc ggcgcggctt
     2281 atgagacgat ggtcgccgcg tgtcgcaagc gcggcaagtg ggccggtatg ggcggcgtct
     2341 attcggagga gggcctgcgc cgttacatcg agcttggcgc acagatggtg ttggccggca
     2401 gcgatctgag cttcctcatg gccgccggtc aggcgcgaac caaaatgctg cgtgggatat
     2461 aataaatcgg aaagtcctaa actggtttac tcaccgccat ttccgcggaa gcaggaatgc
     2521 aggatgcgtt tcagtagaca tgtcactgaa ggcctgcaca aagtgccctg caataactag
     2581 agcttttcga tccaggtgga aacgccaaca gcaagcactc cgttatccac tcccctaggg
     2641 agaggtcggc ccgaaggccg ggtgacgggc tgcaacagtt gccaccggcg gcccccctca
     2701 cctgaccctc tcgccaccgg gagagggaat tgggcgcgag cttgacttag gcgtgcttct
     2761 gtcaaaatcg gatatgctct ataccgacgc tggctcgcgg ggacgcgaaa gcgtctcgat
     2821 ggcgtcggca aggtgctcgt cagtgatatt ataggcaccc ttggccagcc ggatcttatg
     2881 ggcctcacgc aacacgtcgg catgtgtgta gccggcgggc ggctcgaact tgtagcaggc
     2941 gagctcgagc agctggccga gtgggtcgcg aaaatagagc gaatacatga agccgcggtc
     3001 cacctcgccg gagttggcga agccgcgctc gcgcagccgg gcggccgcct gcgtgtaggt
     3061 ggcgcgtgag accgtgaacg cgatgtggtg gacgttgccg acgtcggtcg gattgggccg
     3121 cggatccggc tgccaggact cgttggtgaa aatcgtgatg agccgcccgt cgccgggatc
     3181 gaaatagaga tggttctggt cgggcgcatc gaggttcggc tgctcgaaga cgagcggcat
     3241 gccaagcagg ccttcccaga agtcgatgga agtctggcgg ttggccccga ccagcgtgat
     3301 gtggtgtacg ccctggcatt gtatgcgctg cattgaacgc ctccactttc agtttctcat
     3361 ggtcagcagc gcctacgcac cttcgccccg ctggtcggct tcgaattcct gctcgagact
     3421 tcgctcgagc tccacgaggt cctgcggcag cggcatgcca taggctttga gctcgttcag
     3481 cttccgcttg atcacgaggt agatctcgtg ccgatcgtcg ggctgcgtct cgttttccat
     3541 ctgggtgagc aggagaccga gctccgcctg cacgtcttca aaggccatgg cacatccttc
     3601 gatatgatcc gctgccccgc aaataagagg ttacactctt taacgcgaaa gggcagcgaa
     3661 tgatcgctcg ccacgccttg tctcaagcag cctccagcac caggctgatg ccctgtccca
     3721 cgccgatgca catggtgcac agcgcccgcc gcgcattccg ctctttcagc tccagtgcgg
     3781 ccgtgagggc cagccgggca cccgacatgc cgagcggatg cccgagagcg atggcgccgc
     3841 cgttcggatt gacgtgctcg gcatcgtctg gaagaccgag ctgccgggtg acggccaggg
     3901 cctgcgctgc gaatgcctca ttgagctcga tgatgtcgaa atccgagatc ttgagggcga
     3961 gccgttccat gagcttgcgc gtggcaggcg caggcccgat gcccatgatc cgcggcggca
     4021 cgcccgcggt ggctgcaccc acgacacgcg ccagcggctt caggccatat ttttcaagcg
     4081 cggcctccga ggcgacgagc agcgctgccg ctccatcgtt cacaccggag gcattgcccg
     4141 ccgtcaccgt gcctcctttg cggaagggtg tcggcagctt tgccagcttc tcgagcgtcg
     4201 tctcgcgggg gtgctcgtcc ttcgccacga cgatcgggtc gcccttgcgc tgaggaaccg
     4261 caaccgcagc gatctcctta tccagccgcc cgtttttctg cgcggccgcc gccctcagct
     4321 gggacctgag cgcgaaggcg tcctggtctt cgcgcgagat ctggaactct tccgcgacgt
     4381 tctcggccgt ctccggcatg gagtcggtgc cgtacttctc tttcatgagc ggattgatga
     4441 aacgccatcc gatggtcgtg tcgaaaatct cggcctgccg ggagaaagct tcggtcgcct
     4501 tgcccatcac gaagggcgcc cgcgacatgc tctcgacccc gcccgcgatc acgacttcgg
     4561 cttcgcccgt acggatcatc cgcgcagcct gggccagggc gtcgaggccc gatccgcaca
     4621 ggcgattgac cgtcgtgccc gtcacttccg ccggcaggcc cgcgagcagc gctgacatgc
     4681 gcgcgacgtt gcggttgtcc tcgccggcct gattggcaca cccgaagatc acatcgtcga
     4741 tcgcggttgc tgggacggag ggcaggcgcg ccatcagctc ccgaacgaca tgagcggcga
     4801 gatcgtccgg ccgtacgctg gaaagactgc caccgtaccg cccgatgggc gtccggatgc
     4861 cgctgacgag ataggctggt cgcatgagct cactccaatt tgccgggcgc gaattgccag
     4921 gagactagcc cagattgagc gccgaggaac tgatactggg gaagcgatcc gcggcgcaag
     4981 ctaagggtcg cccccagcta tgcagctgag gcatgttgct aaagcgcggt ggggcgagcc
     5041 gcggacgcat aagcctcgac aagagccttc gcctcccgtc gcacgtcatc gacggtcttg
     5101 cccggcctat aaagcgtcga gccgatgccg aaacccgccg cgccggcctt caggtaggcg
     5161 gccatgttgt gcgccccgat gctacccacc ggcagcaagc gcgtgcccgg cggcaggaca
     5221 gcgcgcagcg cctttacggc gctgggcggg atagcttcgg cgggaaagag cttcagtgca
     5281 ttggcgccgg cggcaagcgc ggcaaacgcc tcggtcggcg ttgcaacgcc cggcaggcat
     5341 gcgaggccct ggcttttggc ggcggtaatg acggcggggt cgctatgcgg catgacgatg
     5401 aggcgtccgc ccgccccggc aacgtcatcg actgcggctg gcgacagtac cgtaccggcg
     5461 ccaatcagag cggcagcacc aaaccggtcc gccagaatgc ggatcgagtc cagcggctcg
     5521 ggagagttga gcggcacctc gatgatccgg aagcccgtct cgaccagtgc cgtgccgacc
     5581 gcttcgcact cggccggtgt aatgccccgc aggatcgcga tcagcggaca ttcggagatg
     5641 gcgttatcga ggtccatgtg acacctccag aaggcctgcg gcacgtgcca gcagcaggtg
     5701 ccccgccacg acactatggt cgggtccgcg gtgccaggcg aaaccaagag cttcggccgc
     5761 ctttccgtag cggtccgtca gctcggcact cccgaagatg gtgatggact gaccgggttt
     5821 tgcggcggcc atgacctccg agccgatcag cagaccggaa aggtatccag acagctcttc
     5881 ggccggcagc atctcgaaca gccccaaggt tctcacactg aacagctggt gcaggaggcc
     5941 tcctcctgac gtcccccgcc gcgcggcttc gacgccctgg aggaaacccg ctcttcgatg
     6001 gtcaccgtcc ttcatgagcc ggccgagaat ggtgtggttc ttgagggcac cgaacacttc
     6061 gcccgtcata taggttgcaa acccggtgat gatgccgtct gcgatggtca cccatttcga
     6121 atgcgttccg gggtggacga acgtggcatt ctgcgtcgac agcaacgcca tggcgccgat
     6181 gatctgcgcc tcctcgccgc gcatgacgtc cggcggctca ccttgcggca tgtgatcgag
     6241 cccggggaca aggtgaacgg tgccgatgcc gtcgacctcg aaggttgtca gcgcggccgc
     6301 gatgtcacga atgcctgcgg ggcagcgcac gtacggcgct tcgacccagc cctgtcggct
     6361 gccgatcatg cccgacatca tgaccggcaa cggtccgtgc gcgctgatcc agccagcgat
     6421 ctctgctctc atcacatctg cgaaggctgc gttctggacg gcgagaatgc ccctcggact
     6481 ttctgtctgt gcaaggatgg tgccgttctt gtctgcaaga taggcgcgga agttggtcgt
     6541 gccccagtcg acggcaatca gatgcggttt gctcatgcgc gcccttcaag agtgatcgag
     6601 tcgggccagt ttattctgaa cccgatgccg ttcaccagcg gctccacctt gtgcgcgcgg
     6661 caagtcctgg aggaatggag cggggaatga ttgtttgcag cgcccgttct cctacaggag
     6721 cctggtgagc aggtgacgac atggtcagaa gcacgccacc gaatacgagc atcaggccga
     6781 taccctgcgc tggcaccacc gcttcaccca ggaatgccac gccaaacagg acggaaaacc
     6841 cgggtgacat gacttgtagg acggccgcaa tgtgcgctcc ggccagccgg acgcaggtga
     6901 agaacagcgc tgtcgcaccg attgccacca gccagccgtg atagacgatt tgcagcgcga
     6961 tctcgcccgc ggtggccggc agttcggatt gcgggctgcc gatcaggtag atcggcagaa
     7021 atatcggcgc gagcagagcc agtgcaatgg ccaatccgat gggatcaacg cgccagcact
     7081 tcacgcaaga attgagaaga gcccaggcag ccgccgccac ggcgaatatg agaaaggcgc
     7141 tgaacgaaag atccaatatt tcgctgccga aactgaacag cgccagtccg gccaggaccg
     7201 cagcggaacc aatcaggcca gcccgcggta gcgcgccggt gtgcagatat tcgagcccca
     7261 acgcgaagac cgggagcagg ccggacaaga gcagtgcgcc tccaccggcg gtggcgtgct
     7321 gataacctgc aacgaggagg ccgtgataga gcggtccccc gagaagcagt aaagcgacga
     7381 ggcgcggcca gccgatggtc agccgcaccc gatcgcgaaa tggggccacg aggatcagag
     7441 caattggaaa gcagccgacg taacgcagga aaaccaggct tatgggatcc aagctcgttg
     7501 ttacgagatg gcgcgaaacg accgctccgc ctccccagat cgcggctgca gccagaccgc
     7561 agagcacgcc gatccaggat tgcccttttg tccgaatggc agctcgcacg tccagggcgt
     7621 ctgctcctct tgcatcaaag ggcgcgcagt cgagcgactg cgcgcttcgg gaaggtcaag
     7681 ccgccgcggc tgcggccgag atcatcacct cttcggagaa gtgctcgtct ccggcctccg
     7741 caatgaaccg cttcaagcct ttcacgaaat cgatcagctc gagctgcacc agcgtggcgg
     7801 agacggactc gtcactgcag agccggtgca tcgtgtgcat ggcggcttcg ctcgtgtagc
     7861 cccagatggt tttgaaacgg gcgttggcct cgtcattttc actggcgatc ctgtacgtgc
     7921 cgccatagcg gacgcccttg atctctgggt cgccagccat ccggctttcg ctgaggagga
     7981 tttcgcgtgt ggtgagccag tctgtaaaat cctctggact cgtcacgctg tagcgccatg
     8041 acacgacgac cggtgagatc atcacccgct tcttcagcaa ctcctcggca tgggcgccga
     8101 gcatgtacgg attgaacggg acgctgagac ctgtccgcac ttggaagttg acaggctcgg
     8161 caaccggatg atccgttgaa ggctggcttt tcatgaaact ggcgaacggc acgctcagcc
     8221 cggtcctcac ctggaaattc acgggctcgg tgctcggcgc ggacgccagc ccgccgccgg
     8281 ccatatgact tccgaacggg acgctcaagc ccgtgcgaac ctgccagttg acgctttcca
     8341 gcgccggcgt ttccgtcgag cggctctctt tcagtttctg tgcaagcctt cggccaatcg
     8401 atgacatgct ctcctccact ccgtgtttga gatgtcaacc tgttgggaaa tagggggcgc
     8461 ttgatgcgcc tcttgcatca gtcgttacgc aaatcactcg atcgcactga tgatgtttcg
     8521 gctcaaaagc tcatcgaagc cggcgtgaaa ctgggctttg gccttgtccc gacccacgcg
     8581 cggccccagc gtcgcgacgt aatcggcctc gatttgctga aaggggcggc cgtcacaaag
     8641 ctcgaggatg aaccaggcgc tcgtgttgag gtcgtagacc tccggctcgt ccggcgtgaa
     8701 ggcgtagcag cgcccccact cctcgaaccg tctgagcacg acgttctcag ttttcgtgta
     8761 gcagccgttg cgggagaaca cggaggtcca ctccttcgtt ggtgtgcacg cagtagggat
     8821 cgggagccgt ccagcggccg tggaaggcgt acgcagtcgc gcggcagccg ccgaaagtcc
     8881 cttgtgtggc cgagcattcg gggcaggagc cttcgaccga attggagcgc agctcccggt
     8941 aaagcgggtg attccatgtc tcgacgaatg gcgtgtcgag aatgttgccg tagttcacgt
     9001 actcgcgcag atacgggcag ccgatcgagt cgccctgcgg gctcaccgcc gcgttctgcc
     9061 agcagcagtt cccgtccctc ggatgccagg actgcatgag ctgaacgccg ggcgggattc
     9121 ggaggccgtg cagcgcccgc atcatttcgt cgagggagag cgaaagctcg gaccaattcc
     9181 gcttggcgcg tccgagcggg taaagccgca ggacgccgac ctgatcgcag ccaagctcgg
     9241 ccgcgagatc gagatagcgc tgcagatgct cgtgattctt gcgcgaaagg atgccgagaa
     9301 tggtgacttt gatacctgcg gcgcgcgcgt ttcgtattcc tgcaattgtt gcatcgtagc
     9361 tgcctgactg ccccgtcagc tcgtcctgca cttcggggat ggcagcgaaa agatcgacat
     9421 agagccgccc gacaccgagc tttttcaagc gatccgcgcg tgccttgtcg atcagcgtgg
     9481 catgcgtcct cacggcaacg tagaggcggc gattgcagtg ggcgagaatg tcctcgaaat
     9541 caggccggtg aaacggctcg cctccttcaa agaagatatg gatgaattct tcggcgacga
     9601 gctggtctat gaaagttttc cattgctcgg tcgtgagctc gggacgtttg ctcgagccac
     9661 agtccgcgta gcaaagcttg cagttcatgc tgcaagtctg ggtgacgcag atcgtcagct
     9721 tgaacggctt ttcgagtctc ggaacgcgtc taggctcatg tgcgccgtca aatcggacca
     9781 gttcgttcac cctctcacct ccttcagcgt atccgcgatg ttcacagggc cgaagccgaa
     9841 gcgcggactc caggtgtcgc gccgcgggac cagcaggtcc gtccatcgtt gcggttcaag
     9901 gcctcgtctc gatgcgaggc attgcattag aagggccatc gccccggatg tgagcgcggc
     9961 cgccgcactc gttccgctcg tttcgacaaa tcccgcagtc ttggagcgcg cgccccagat
    10021 gcgatgggcg ggtgcacaga tgagatttgc ggcgtctttg gttaccgccc acggcagcgg
    10081 tccgaggctc gacgccggaa acggatctgg atttgcggca gtgcgatgat gatcgtaacc
    10141 cgcagcgatg atcgcgcctt cggccgctgc gagactcgcg agcgtacttt ctgcgtaggc
    10201 cggatagagt gtcgagcggt tcgcgagacc atcatcccgc tcgacccagg catgaatgtc
    10261 tgctggacaa cttccggtcg cggagcagtt gatttccagg gacagctcgc cggaatattc
    10321 atgccagaaa tgatccggca cgtaaggcgg atgaaccgca atcctgatcc gcgaccgcgg
    10381 gctgcgcgca aacggcacgt gatcggcaat gccgatgcgc gtaccgcaga gttcaatcgt
    10441 gtgagtgcgc cccggagaaa tgctccatcc cgggtggcca gggtgtggtg aaacgctgat
    10501 ttcgagaggc gtttctgagc gataccaaat ttccagcttg ttttgggtcg gatcatcccg
    10561 gtgcatgcgc cacgcgaaag acagcggcct gcccggttca gctttgccac cgaaatggcc
    10621 ttcttccagc cctgcatttc cggcacccac cacgactgcg cagggcagat cagactgcaa
    10681 acagcgccct gtgaggtcgg agatggcttg ttcgactcta gaacggccgt cgtgcgcgcc
    10741 cgcgtaggcg ccgatgctca aattcactac cagcccccgg gcatttaagt gaactgcgcg
    10801 cgcatagatg tattccagcg cgctgacgat ctgcgccgcc tcgtcgtagc ttcgccagcc
    10861 gttccacacc ggctccattt ccggccgcca atggcgccag gtcggcagac cgttttcgtc
    10921 ttcctctctc caatgatgat cgaggaggcc gagctgaacg gcgatcaggt ccgcagcagg
    10981 tgcgacaccg cgaaatccag cgaccgggct ggccgcggca atggcggcca tgacggtgcc
    11041 gtgcgcgccg ctttgaacgc cgtacctccc gaagtaattt gcatgtgggt catattcggc
    11101 gtccgccgca tctcgcgagc cggtccggtc ggacgcggca atgaggcggt cgacgtcgcg
    11161 cgcatcgaat tctcggccgg tattctgatc ccacagatag cgaaagcgcg tttgcccggt
    11221 tgcagtgtac cgaaagcagg gatgcagaat gtcgaagccg taatcgatta cgccgatgac
    11281 gataccgcgc ccgtcgatgc cgctatccag gggaggtaca cccatttgct gcagggcgct
    11341 ggcgttgaat ggctgcgcac ggtttgccat gaggcactcc ggtggctcgg gaagatcgag
    11401 cggctggagc ccttgcctca tgccggcgcg ggctcagcac gctcttggta cgcagcacag
    11461 cggtcgtgaa actatcgccc gatgcccctg ctgtgttccc gggcaattac ccaggtgcac
    11521 gtgccggctt ttcgtgagaa gtggttcagt tcatgcgcag gcatagtcgg tgcggatgcg
    11581 ctcatttcgg tgcggaagct acgcgcccgg atttttttgg aatgccggta gcctggcgtc
    11641 gatcccaatg acagccagag gtctcggttc ggtatggctg gtccgcgctg agcggcttgc
    11701 ggcgtccgta ttcaggccat aggctccgga cgcgagcggc ggccgcgccg tttcgcgctg
    11761 gaattgtttt ctctatggag accccatgtc cgtggtcctt gccgagcacc cggcggaggg
    11821 tgttctgctt ctgcgcatca atcgccccga ggcgcgcaac gccctgaacc tggaggtacg
    11881 cagggcgctc gtcgccgagc tcaaggctgc tgccgaagac cagcagattc gggctgccgt
    11941 cctcacgggc aacgataagg ccttcgcagc gggggctgac ctgcgcgaga tgcgcgatct
    12001 cggtccgatc gaggcgatgc agcgcggcac ccacaagctt tgggacgaga tcgccgcctt
    12061 ccccaagccg ctgatcgccg ccgtgaatgg ctttgcattg ggcggcggct gcgagctggc
    12121 acttcactgc gatatcatca ttgctggcga aggcgccaag tttggccagc ctgaggtgaa
    12181 ggtcgggatc ctgccgggcg gctccggaac ccaacgcctc gtccgcgccg tcgggaagta
    12241 caaggcgatg ctgatgatcc tgaccggcga gatcatcggt gctcgcgagg cgagcgaaat
    12301 gggcctcgtc agccgggttg tcgccgacga tcaagtcgtc agccacgcca tcgagatggc
    12361 cgccaatata gcgaagctgg cgccgttggc cgtcgagctt gccaaggagg ctgcactgac
    12421 tggccaggat gcgtccctcg ccacgggtct cagcctcgag cgcaaggcgc tgtggctcgc
    12481 gttcgccacc gaggacaagc gcgagggcat gaccgcattc ctcgaaaagc gggcgccgaa
    12541 cttcaaggga aaatagaaga tggcactgga ccccaatcgc gaggatctcg tcgtcggcgt
    12601 catcggaacg ggtgccatgg ggcgcggcat catgcaggtc gctgcccagg gcggcatgca
    12661 ggtctttgcc tacgatgaga agtccggtgc ggccgagtcg gcggtgcagc acataaggca
    12721 gatgctggac ggattggtcg cgaagggccg gctcgacaag gcgggcgctg atgctgccgt
    12781 cgcccgtatc gtgatcgccg atggccttgc gcaaatcgcc actgcggacg tcatcatcga
    12841 agccatcgtc gagcagctgg gcatcaagca ggagctgttc gcgacgctcg atgagctggc
    12901 cggtcccgat acgatcatcg catcgaatac atcatcgctg ccgatcaccg cgattgcggc
    12961 gcgctgcaaa cggccggagc gcgtcgcggg tatgcacttc ttcaaccccg tgccgctgat
    13021 gaagctggtg gaggtcatcc cgggcctgaa aaccgacccg caggtcatcg aggcgttgac
    13081 cactgtcgcg cgccgcatga cgcgggtgcc cgtcgtctgt accgacagcc cggggttcat
    13141 cgtcaaccac atcggccgcg cgtacgtgcc ggaagcggcg cgcatcgtgg ctgagggcat
    13201 cgccgagcct gcggagatcg accgcatcat gaccgaggcg gtcggcatgc ggatggggcc
    13261 gttcacctta ctcgatctcg tcggagccga tgtctcagtg tcggtgatgg agagcctgtg
    13321 gagccagttc tatagcgagc cgatgtatgc gccgcagccg atactgaaat tgcgggttgc
    13381 cggcgggctc tatggccgca agacgggagc gggctggtac acgtatgagg acggcaagcg
    13441 cgtcgagccg ccgcgtccgg agcctccggc ggctcggcct cgttcggtct gggtcagccc
    13501 acgccacccg gccgccagcg ccaaggcaat tgccttgctg cagcggctcg gagccaacat
    13561 cgagaacggc aacgcaccga gcgccgaggc gctgtccatc gtcacgccaa ccagtggcga
    13621 ctgcagcagc gaaatcgtcc agcaaggcct cgaccgcaca cgaacggtcg cgctcgacac
    13681 cttcttccat ttcgagaagc accggacgct gatggtgccg cctgggatcg atccgagcat
    13741 gcgagaggcg gcacacgggc ttctcgcgtc ggatggtgcc ggggtcagcg tcatcaacga
    13801 cagtccgggc ttcgtggcac ccaggatcgt cgcgcacatc atcaacgtcg gctgtcaagt
    13861 cgcgcagcag aagatcgctt cgccgagcga tatcgatttg ggggcgaaac tcggcctctc
    13921 ttatccttac ggacccctgg agtggggtga tcatctcggt cccgactggg tgttggagat
    13981 ggtcgcagag ctggagcgct tctacgagga gccgcgctat cgcccttcgc cgtggctcaa
    14041 gcgccgcgcg ttgctcggtc tctccctcac gacaccggac gggcggcact gagcgtcccc
    14101 ccaagcccgc aaaatgacat ccgaggcaag gccaatcagg tgagcgactt gaacaaggac
    14161 tcgtggcagc ggctgtccgc tgccatgcct atgctttgga ctttgctgct gacgctctgc
    14221 gttggcgcgg ctggcggtgc cgtattcttt ttgcttgggc tgccagcggc gtggctctcc
    14281 ggtccggcgg tatcggttgc cggcgcgagc ctcgccgggt tccgcctgtc cgttccgaac
    14341 cttgcccggc aagcagcatt cgtgtttctg ggctccacga tgggcgcgac gatgacgccg
    14401 gagacgctgg cgctgatggc gcgctggccg gtgagcctgg caggcctggc cttttgcctc
    14461 ctgggcatta tggtctgctg cagcgcctac ctgcagcacg tgcacggcta tgatcgaaca
    14521 acggcgcgtc ttgcggcggt tcccggtgca ttgccttacg tcttggcgct ggcggcggag
    14581 agccggggcg accagtaccg tatcgccatc atccagatct tcaggctcgc tgcgctgctg
    14641 atattcctgc ccaacgtgct gtcgctgttc ggatacgaag cggagtccct cgctcccaag
    14701 acctcaactc cggtgaattt gctcgaagtg gcgctgatgc tggtgggcgg ggtcgcgggg
    14761 gccgtcgttt tcgcatggat caaggcgccc gcggcgagct tgttcggctc gatggttgcc
    14821 gccggattgc tctatgcgag tgggaccctg tcatcgcccg tcccgggctg gctgatgctg
    14881 cccggcttcc tcgtgatcgg cgcgacggtc ggcaccaact tcacgggcat cgaccgacgt
    14941 ttgatcttgg aaacgctgct tgccagcatc ggtagcctgc tggtgggcac tgccatcgcc
    15001 ttggcgttcg ccgtacccgt ctcatggctg ctcggcctga cagtggcgca actctggctg
    15061 gcctatgcgc ccggaggcgt cgacgtgatg accatcatgg cgatggcgct gggcctcgac
    15121 cccgccttcg tgggcgcgca ccatgtggcg cgattcatcg ggctagggtt tttcgtgcct
    15181 gtctggatgc gagcaggcct ttcgcaggag gagcggcgcg gctagaaaac cgcgcctctg
    15241 gctttgtcgg cagaaggcta tttcaggccg ccttcttcac ctcggccgtg atcttctggg
    15301 cggcgtcggc gagattgtcg gcagggacga tcttgaggcc gctttcggcc aggatcttct
    15361 tgccgaggtc gacgttcgtg ccctcgagcc ggacgaccag cgggacgttg atcgccatct
    15421 cgcgcgccgc agcaaccacg ccttcggcga tgatgtcgca acgcatgatg ccgccgaaga
    15481 tgttgaccag aatgcctttc actgccgggt cggagaggat gatcttgaag gccgcctgca
    15541 ccttctcctt cgatgcgcca ccgccgacgt cgaggaagtt ggcaggctcc tcgccgtaga
    15601 gcttgatgat gtccatggtc gccatcgcga ggccggcgcc attcaccatg caaccgatgg
    15661 tcccgtcgag cttgacgtag gagaggtcgt acttggacgc ctcgacttcc atcggatcct
    15721 cttcgtcgag atcacgcagc tccacgatct ccttgtgccg gtagagcgcg ttggaatcga
    15781 aattcatctt ggcgtcgagg cagaggagct tgccgtcctt ggtgacgatc agcggattga
    15841 cctcgagcag gctcatgtcc ttttcgatga agaggcgata gagatcgccg accatcttca
    15901 cgcactggct gacctgatcc cccgaaagtc caagcgcgaa cgcgatcttg cggccatgga
    15961 acgggaagta gccggaggcg gggtcgatcg acagcgttac gatctgctcc ggcgtgtcgt
    16021 gcgccacctg ctcgatatcc atgccgccgg cctgcgatgc gatgaaggcg atgcggctgg
    16081 tggtgcggtc gacaagggcg gagagataaa gctcacgctc gatttgtgag ccgtcctcga
    16141 catagagccg cttgacgaca cggccggccg ggccggtctg cactgtgacg agggtattgc
    16201 ccagcatttg ctgagcgaac gtgcgcacct cgtcgatcga tctcgcaagc cggacgccgc
    16261 ccttgtcacc tgccgcagct tccttgaact tgcccttgcc gcgcccgccg gcgtggatct
    16321 gcgccttgac cacccacacc ggccccggga gcttctcggc cgccgcgacg gcttcctcga
    16381 ccgtgaatgc cggatggcca gtcggcgtcg gcaccccgta ctgccggaac agctctttgg
    16441 cctgatattc gtggatgttc attgcacgat tccctgtaat gcgaagtccg gggtcatata
    16501 cgccggctcg cctacttcct gtctcccgcg tcgggggttc aggtcacagt tccgctaggg
    16561 aattgctcgt agggcgagtc gcttctgaca gcaaacctgt tttcgatggc ggtttcgttg
    16621 tggcgcgtcc tgggttcccg catcgcggga atgatgggtt tgtcaggtat tcctctgggc
    16681 gatggcctcg atctcgacgc gggcgccgag aggaagggcg gcgacctgga tcgtcgagcg
    16741 agccgggaaa ggggcgacca tgtactcggc gtagacttgg ttcatggcgg cgaaatcggc
    16801 catgtcgacg aggaacacgg tcgtcttcac gacatgctcg aagccgaggc cggcagacgc
    16861 caggaccttc ttgaggttct ccagacactg gcgcgtttgc gtgccgatat cgccttccac
    16921 gagcttgccc gtctctccat cgatatggac ttggcctgag agataaacga gctgcccggt
    16981 gtcaatggcg agggagtaag gcccgaccgc gcgaagtggt gcagcttcaa tcgccttctt
    17041 tgacattgtc tactccgtta atctgaaacg ctgcgggggg cctcggaacg acagccgtca
    17101 cgtctccagc ttggcgtcca tggtaatttt tgcgttgagc accttggata cagggcagcc
    17161 ttccttggcc tcgttcgcgg ccttctcgaa ggcggcctgg tcagcgccgg gcaccttcgc
    17221 cacgacatcg aggtgaaccg cggtgatcgc gaagccgctg tcgaccttct ccagtgtgac
    17281 tgccgcctcg gtcctgatgc tttccggcgt gagcccggcg tttcccagct gcagcgagag
    17341 tgccatcgag aagcagccgg cgtgggcggc agcgatcagc tcctcggggt tggtgccggc
    17401 atcctgattg aagcgcgtcc cgaatgaata cggggcgttg gtgagcagtt tgctttgggt
    17461 cgagatcgta cccttgccgt ccttcagtcc gcctttccac tctgccgatg cggttcgctt
    17521 catgtgaggt ctcctgttca gccaggttga tcccccctta aggaagctca ggctacgagt
    17581 tgcggtgcga tgcgcgtgca ggcttcgacc agacttttca ccgaatcgac ggacttggag
    17641 aacatcgcct tctcttcgtc attgagctcg atttcgacga tgcgctcgac accgccagtt
    17701 ccgatgacga caggtacgcc gacatagagg ccccgcacgc cgtactcgcc attgaggaag
    17761 gccgcacagg gcagaacgcg cttcttgtcc ttgaggaagc tctcggccat cagaatcgcg
    17821 gaggatgccg gcgcatagta ggcggagccg gatttcagca gagcgacgat ctcgccgccg
    17881 cccttgcgcg tgcgctcgac gatctggtcc agcttctcct gcgacatcca gcgcattttc
    17941 accagatcgg gcagggggat cccggcgacg gtcgagtagc gcacgagcgg caccatgtcg
    18001 tcgccatgac cgcccagcac gaacgcggtg acgtcctcta ccgagacctt cagctcttcg
    18061 gcgaggaaat gacggaagcg tgtgctgtcc agcacaccgg ccatgcccac gacgtggctg
    18121 tgcggcaggc ccgaggcctt ctgcagggcc cagaccatcg cgtccagcgg gttggtgatg
    18181 acgatcacga aggcatcggg cgcgtactgc ttgatgccct cgccgacctg ctgcatgacc
    18241 ttgagattga tctcgagcag gtcgtcgcgg ctcatgcccg gcttgcgcgg tacgcccgcg
    18301 gtgacaatgc agacctgagc gccaacgata tcggcatagc tgttggtgcc cttgagcaca
    18361 caatcgaagt cctcgacacc gccagcctgg gcaatgtcca gagccttgcc ctgcggaatg
    18421 ccctccatga tgtcgaacag gacgacatcc cccag
//
LOCUS       DASVDU010000117         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_396031, whole genome shotgun sequence.
ACCESSION   DASVDU010000117 DASVDU010000000
VERSION     DASVDU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396031"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VF226_07425"
     CDS             <1..399
                     /locus_tag="VF226_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073856.1"
                     /translation="AVVAGLCFPPLSPRSIVIEEVELVIWLHIYRIFDLPYQSQIVPL
                     AAILADIGDAWEHEANRAKLVQWYWNGVFGELYGSAVETRIAKDVMEVPRWLSGGPEP
                     STVSETIFRSDRLKTMRMRLSAAYKGMNAC"
     gene            complement(375..725)
                     /locus_tag="VF226_07430"
     CDS             complement(375..725)
                     /locus_tag="VF226_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073857.1"
                     /translation="MPDSVIWAIFSTLNDHQEINDQSIVQSFVASGGMTRAQLVLRTA
                     VGFSLKEGERIHWKSVGKNLDAILRSEDRPAPVLWEDAVNIHVLPEKYRMVELLARPE
                     ILRAFLHQQAFMPL"
     gene            complement(760..1866)
                     /locus_tag="VF226_07435"
     CDS             complement(760..1866)
                     /locus_tag="VF226_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX7073858.1"
                     /translation="MPYIHELPGWPELSWDHKVLAEPLAAVRHRQGRLIGRMEALGFE
                     LRSEATLQTLTEEVLKSSEIEGEILDRNQVRSSIARRLGMDIGGLVPADRDVEGVVEM
                     MLDATQNYRAELTDERLFGWHAALFPTGRSGMHRITVGAWRDDKSGPMQVISGPVGRE
                     RVHYEAPKAARVADDIKAFLGWFNGQDGIDPVLKAGVAHLWFVTIHPFDDGNGRIARG
                     IADMQLARSEKSPQRFYSMSAQIRHERKAYYEILERTQKGDLDITEWLTWFLECLSRA
                     FDGAESILAGVLRKARFWDRHPGDALNDRQRAMLNRLLDGFEGKLTSSKWAKIEKCSP
                     DTALRDITDLLQRGILVRDEGGGRSTSYSLVDVA"
     gene            2266..2892
                     /locus_tag="VF226_07440"
     CDS             2266..2892
                     /locus_tag="VF226_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073859.1"
                     /translation="MTTALQSRLMDPALLEEFCREYTRYLNRLRAEKNAALGSAKSEL
                     ATLLKERENIIQAIRDGVPASEMKDDLARITVRREELEALLAGTKEVPVLLHPNMATH
                     YRSQVARLVAILNDERNRAEAADILRSLIDRIELTPDAEGKLEIDLYGDLAGILSLAA
                     NKKGPLDESGPMQDEVVAGACKRFCYNFWPERPPPVGNEVQNPHQLAA"
     gene            complement(3011..3409)
                     /locus_tag="VF226_07445"
     CDS             complement(3011..3409)
                     /locus_tag="VF226_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017170488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7073860.1"
                     /translation="MKITVDTNVLLRAVVEDDPGQQLAAVEALERAELAAVSLQSLCE
                     FAWVLERSYGVARADIAASVRAVLDIRNIAVNRPAVEAGLSVLEAGGDFADGVIAYDG
                     RWLGGETFVSFDKKAVRLLTAQGQAARLLG"
     gene            complement(3406..3672)
                     /locus_tag="VF226_07450"
     CDS             complement(3406..3672)
                     /locus_tag="VF226_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7073861.1"
                     /translation="MTTLTVTARGQVTFKKDVLKHLGIQPGDKIHLDLLPDGRAELKA
                     ERPQGSWRDLRSVLKGKTNGARLSIEEINEAIAEAGATAGLGRT"
     gene            complement(3871..4245)
                     /locus_tag="VF226_07455"
     CDS             complement(3871..4245)
                     /locus_tag="VF226_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073862.1"
                     /translation="MNKRATESELRLTRLYDAIEAGIADLDAPALKDRIDGLKAIRDQ
                     AKADADRAQAMLQNSGQKAVTPQMLRKFAATARERIRLDGGGYRRDHLRALAQRVEVA
                     DGEVRIWDRSPGCYRRSWREVA"
     gene            complement(4242..4649)
                     /locus_tag="VF226_07460"
     CDS             complement(4242..4649)
                     /locus_tag="VF226_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase zinc beta ribbon domain-containing
                     protein"
                     /protein_id="HEX7073863.1"
                     /translation="MSEIVTVPVPPIIDKETFEAVQKLLKARNPKVTPARIIGGPTML
                     TGLIHCAKCGGAMTIRTGKGGRYRYYACSMKARQGPIACEGMAVPVEKLDDLVVNHLG
                     PSFSNPSGWKPSLPPRSTAGKNMPSAAASISLS"
BASE COUNT          866 a         1519 c         1442 g          937 t
ORIGIN      
        1 gcggttgtcg ccggtctctg tttcccgccg ttgtcgccgc gatcaatagt cattgaggaa
       61 gtcgaactcg ttatatggct ccatatctac cgcattttcg atctgcccta tcagtcgcag
      121 attgtgccgc tggcggcgat cctcgccgac attggggatg cgtgggaaca tgaagccaac
      181 cgggcgaagc tggttcagtg gtactggaac ggcgtgttcg gtgagcttta cggttctgcc
      241 gttgaaacac gcatcgccaa ggatgtcatg gaagtgcccc gctggctgag cggcggcccc
      301 gagccttcga cggtcagcga aaccatcttc cgctccgacc gcctgaaaac catgcgaatg
      361 cggctttcag cggcctataa gggcatgaac gcctgttgat gaaggaaggc gcgcaggatt
      421 tccggtcggg ccagaagttc gaccatacgg tatttttcgg ggagaacgtg gatattcacc
      481 gcatcttccc acaggactgg tgccggacgg tcctcagatc tcaggatcgc gtccagattt
      541 tttccgactg atttccagtg gatgcgctcg ccctccttga gcgagaaacc gacggcagtt
      601 cgcagcacga gttgagcgcg cgtcatgccg ccagacgcga caaaggattg aacaatgctc
      661 tgatcgttga tctcttgatg gtcatttagg gtcgagaaga tcgcccagat caccgaatcc
      721 ggcacgcagc cggtccgagc acccgccgca tatggttcgt cacgcaacgt ctaccagcga
      781 atagctggtg ctgcgccctc ctccttcgtc cctcacgaga atcccacgct gcaacagatc
      841 ggtgatgtcg cgcagcgccg tatcaggcga gcacttctct atcttcgccc acttggaaga
      901 tgtgagcttg ccctcgaacc cgtcgagcag ccggttcagc atggcgcgct ggcgatcgtt
      961 gagggcatcg cctgggtgcc tgtcccagaa gcgtgccttt ctcagcaccc cggcgagaat
     1021 gctttccgcg ccgtcgaacg cgcgcgaaag acattcgaga aaccacgtca gccactccgt
     1081 gatgtcgaga tcgcccttct gcgtgcgttc gaggatctcg tagtaagctt tgcgctcgtg
     1141 ccggatctgc gcagacatgc tgtagaagcg ctgcgggctc ttctccgagc gtgcaagctg
     1201 catatctgcg atcccgcgcg caatgcgtcc gttaccgtcg tcgaacgggt gaatggtgac
     1261 gaaccacaga tgcgcaacgc ccgccttgag gacgggatcg attccgtcct gcccattgaa
     1321 ccagccaaga aacgccttta tgtcgtcggc gacgcgcgcc gccttcggcg cttcgtagtg
     1381 aacgcgctcg cgccccacag gccccgagat cacctgcatc ggccctgact tgtcatcgcg
     1441 ccacgcgccg accgtgatcc ggtgcattcc gctgcgcccg gttggaaaca gcgcggcatg
     1501 ccagccgaac aaccgctcgt cggtcagttc agcacgatag ttttgggtcg cgtccagcat
     1561 catctcgacg acgccctcca catcgcggtc cgccggaacc aggccgccaa tatccatgcc
     1621 caggcgccgg gcgatggatg agcgcacctg attgcgatcg aggatttcgc cttcgatctc
     1681 gctggacttg aggacttcct cggtcagggt ctggagcgtc gcttcgctgc ggagttcgaa
     1741 gccgagcgcc tccatgcgcc cgattagtcg tccctgacgg tgacgcacgg cggcgagcgg
     1801 ctccgccagg accttgtgat cccagcttaa ttccggccag cccggcagct catggatgta
     1861 gggcataatc tccgcactcc ttgcggagat tatgcccctt attctccgca ccggtcaagt
     1921 atattctccg cacaacatgc ggtgattagc cctgataatc tccgcatggg accgcgaacc
     1981 aggctgaaaa cgctccaatg cgtgtgggcg aggcgctgaa aaactggatc gcggccaacc
     2041 tcgattttct cgggcgcaaa tggcagcacc acgtccaaag gttccggcct gcggatgaat
     2101 gtgaggacgg gccggacaat tggttcctgt gggccagtga gcggctgctg ttcggagcgc
     2161 ggcttcagca aggtctcgca gaaccactat ggttgctcga cagcgcgcaa taagggcacc
     2221 tgcgaaaacc gcctcacctt gcggcaggat gagctggaag gccttgtgac cacggcgctg
     2281 caatcccgcc tcatggaccc tgcgctgctc gaagaatttt gcagggaata tacgcgctac
     2341 ctgaaccgcc tgcgggccga gaagaatgcg gctctcgggt ccgccaagag cgaactcgcg
     2401 acgctgctca aggagcgcga gaacatcatt caagcgatca gggacggcgt gcccgcgtcc
     2461 gaaatgaagg acgatcttgc ccgcatcacc gtgcggcgcg aggagctgga agccctgctt
     2521 gccggcacaa aggaagtgcc ggtgctgctg cacccgaaca tggcgacgca ttaccgttcg
     2581 caagtcgccc ggctggttgc catcctgaac gacgagcgga accgggcgga agccgccgac
     2641 atcctgcgct cgctgatcga ccggatcgag ttgacaccgg atgccgaggg taagcttgaa
     2701 atcgacctct acggcgatct cgccggcatc ctgagccttg ctgcaaacaa gaaagggccg
     2761 ctcgacgaga gcggccccat gcaggacgag gtggttgcgg gggcctgcaa gcgcttctgt
     2821 tataacttct ggccggagag acctccgcca gtcggcaacg aggtccagaa tccccatcag
     2881 ctcgccgcgt aggacggctt cgaccgcccc tcgcttctct ccgggcttga tgaccacctc
     2941 gcccacctgt tcattctttt cggtctgccg ggtcatcaag ccttcctgtg gttgcgctat
     3001 gctggatcgt ctatcccaag agccgggccg cctgcccttg agccgtcagc agcctcaccg
     3061 ccttcttgtc gaatgaaacg aaggtttctc cacccagcca gcgtccgtca taggcgatga
     3121 cgccatcggc gaagtcgccg ccggcttcaa gcacgctcaa gcccgcttcg accgccgggc
     3181 ggttgaccgc gatgttgcgt atgtcgagca cggcgcggac cgaggcggca atgtcagcgc
     3241 gtgccacccc atagctccgc tccagaaccc aggcgaactc acaaagggat tgcaggctga
     3301 ccgcggccaa ttcggccctt tcgagggctt cgaccgcggc gagctgctgg cccggatcat
     3361 cctccactac cgcgcgcagc agcacgttgg tatcgacggt gatcttcatg ttcggcccag
     3421 gccggcggtg gcgccggctt cggcgatggc ctcgttgatc tcctcgatgg acagccgtgc
     3481 gccgttggtc ttgcccttga ggacgcttcg caagtcgcgc catgatcctt gcggccgttc
     3541 ggcctttaat tccgcccgcc cgtccggcaa cagatccaga tggatcttgt cgcccggctg
     3601 aatgccgagg tgcttcagca catccttttt gaaagtgact tggcctcggg cggtgactgt
     3661 cagggtcgtc atggcacact ctccgactga acacctccaa cataatgcaa aatcgcctta
     3721 ctttcaaggc ggagaaactg ggcgtgccaa gccggccgac cgctaagcct gggccttcga
     3781 gcttggcggc tcatagggct acagcgcaaa cgtagtgatc atcgctatcg gtcgcggccc
     3841 gccacttcag tccctgagtg ggcaccgagt ttacgccact tcccgccacg agcgtctgta
     3901 gcagccggga cttcgatccc agatgcgaac ctcaccgtct gcgacctcga cacgctgtgc
     3961 cagcgcgcgc aggtggtcgc ggcgatagcc gccgccgtcc aaccggatgc gttcgcgggc
     4021 ggttgcggcg aacttgcgca gcatctgcgg cgtcacggcc ttctgtcccg agttttgaag
     4081 catggcctgt gcccggtcag catcggcctt ggcctgatcg cggatggcct taaggccgtc
     4141 gatgcggtct ttcagcgccg gggcgtccag atcggcaatg cctgcttcga tagcgtcata
     4201 gagccgcgtt aggcgcagtt ccgattctgt ggcgcgcttg ttcaactcag cgatatgctc
     4261 gcggcggcgc tcggcatgtt cttccctgcg gtcgagcgcg gcggcaagga cggtttccag
     4321 ccgctcgggt tggagaagct gggcccaagg tgattgacga ccagatcgtc cagcttttcc
     4381 accggcacgg ccatgccctc gcaggcgatc ggcccctgcc gtgccttcat cgagcaggcg
     4441 taatagcgat agcgcccacc cttgccggtg cggatggtca tggccccgcc gcacttggca
     4501 caatggatca ggccggtcag catggtcggg ccgccgatga tgcgggcggg cgtgaccttg
     4561 gggttacgag ccttgagcag cttctgaacc gcctcgaacg tctctttgtc gatgatcggc
     4621 ggaacgggga cggtgacgat ctcgctcatg ctcggcctgc cgcgccgtcg aaacgcgcag
     4681 ataaagggcg gcgcgcagcg gcacggtgga agcggtcatg gcgatgtctc ctttcaaacc
     4741 cggtcgaacg aacggggccg ctat
//
LOCUS       DASVDU010000118        33999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1385438, whole genome shotgun sequence.
ACCESSION   DASVDU010000118 DASVDU010000000
VERSION     DASVDU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385438"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VF226_07465"
     CDS             complement(<1..497)
                     /locus_tag="VF226_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7073864.1"
                     /translation="MALLIIGSQRSEEFAAAARALNSGLDIRVWPETGRIEDIRYALA
                     WGPPVGVLKTFPNLELIISVGAGVDHLMKDPELPDVPIVRYVDPDLTNRMVSYLVLHV
                     LFHQRRMCEFLELQRERKWEFRPEPGTHEVRVGLMGLGVMGEATAKALKALGFQLRGW
                     SRSER"
     gene            complement(519..2153)
                     /locus_tag="VF226_07470"
     CDS             complement(519..2153)
                     /locus_tag="VF226_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073865.1"
                     /translation="MKTGAPLVQVRDLSVAFRSGDTTNLAVKGVSFEVNKGETVALVG
                     ESGSGKTVSALSIMRLLPYPAASHPSGEIIYNGKDLLKASEADMRDIRGARISIIFQE
                     PMTSLNPLHTIEKQVGEVLKLHRGMDDKAIRQRVLELLRRVGLRDPEKRLTAFPHQLS
                     GGQRQRVMIAMALANEPDLLIADEPTTALDVTIQAQILELLKELQSELGMAMLLITHD
                     LGIVRKMAERVYVMNNGKVVEEGKTADIFADPKHPYTRHLISAEPKGEPPPMNPSAPI
                     VVQTDDLKVWFPIRKGLLRRTVDHVKAVDGLSLKLRAGQTLGVVGESGSGKTTLGLAI
                     MRLISSKGQVAYLGNRIDGLNSKRMRPLRKEMQIVFQDPYGSLSPRLSVGQIIEEGLL
                     IQQPHLSYEERRARVAQALSEVGLDPEAQDRYPHEFSGGQRQRIAIARAMVLEPKFVM
                     LDEPTSALDMSVQAQIVDLLRDLQQRHNLAYLFISHDLKVVRALSSYVIVMRNGKVVE
                     EGPAEEIFRAPKTDYTKALLAAAFDLKVEHRGALAV"
     gene            complement(2161..3426)
                     /locus_tag="VF226_07475"
     CDS             complement(2161..3426)
                     /locus_tag="VF226_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073866.1"
                     /translation="MDIRTDDKLKPIAPSDTAADVETQEKIATTPGFQRQPTTWIGRK
                     RGAAGRFKLSPINRRRWENFKANRRGYWSFWIFLVLFVLSLGAELIANDKPLLVYYKG
                     ELLSPVLVDYPESKFGGFLATTDYKDPFIIDEIESNGWMIWPPIRYSYTSINKDYPRI
                     KGPEDRCLGFPAPPLWATNAQLCEAPADQLARFQELGNRNWLGTDDQGRDVLARMIYG
                     FRISVLFGLLLTFFSSVIGVTAGAVQGYFGGRVDLFFQRVLEIWSSIPSLYVLIIISS
                     VLVPGFWTLLGVLLLFQWVSLVGVVRAEFLRARNFEYVTAARALGLSDRKIMFKHLLP
                     NAMVAMLTFLPFNLSGSITALTALDFLGLGLPPGSASLGELLAQGKANLQAPWLGLTA
                     FFSLAVLLSLLIFIGEAVRDALDPRKTFR"
     gene            complement(3431..4540)
                     /locus_tag="VF226_07480"
     CDS             complement(3431..4540)
                     /locus_tag="VF226_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEX7073867.1"
                     /translation="MGAYLLKRILLIFPTLLGIMLISFAIIQFAPGGPVERVIAQISG
                     TDVSATSRIGGSQGDGLAGQAAQQMGHEGGGSKYRGAQGLDPEFIKQLEKQFGFDKPA
                     HERFFIMMKSFLMFDFGKSYFRDVSVIQLIAEKLPVSISLGLWMTLLTYLISIPLGIR
                     KAVRDGERFDVWTSGFLVVGYAVPGFLVAVLLLVLFAGGSFFQWFPSRGLTSDYWEDL
                     TLAGKIADYFWHLALPITAMALGAFTTMTFLTKNCFLDEIRKQYVITARAKGLNERQV
                     LYGHVFRNAMLLIIAGFPGAFISAFFSGSLLIETIFSLDGLGLLGFESIINRDYPVVF
                     ATLYIFSLVGLVVNLISDFVYTLIDPRIDFESREV"
     gene            complement(4584..6431)
                     /locus_tag="VF226_07485"
     CDS             complement(4584..6431)
                     /locus_tag="VF226_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7073868.1"
                     /translation="MTYAREATPGLRWVRPLAASILLALAAVMPVSADEAPSHGLSAF
                     GELKYGPDFTHFEYVNPDAPKGGRLSMIGPAGTITFDSFNPFILRGDAAQGLDLLFDS
                     LMVRAMDEPDAVYGLIAKSATVAADRRSVTFELRPEAKFADGSPVTAEDVAFTFETLK
                     TKGHPQFRLSLRNVVRAEVLEPHKVRYVFEGENLRDLPLIVAELPVLSKAYYARHEFD
                     QTTLEPPLGSGPYRISEFKQGSFTTFKRREDYWAKDLPVNRGRYNFDEVRYEYFRDRT
                     AQLESLKAGAYDLREEFTSKDWATGYDIPQVKDGRIVLLTLPDESPSGAQGFFINTRR
                     AKFSDPRVRKALDYAFDFEWTNSNLFYSLYKRTASYFENSDLKAEGKPSPEELALLEP
                     YRDRLPPEVFGEPYTPPVSDGSGSDRRLLRTAAQLLTQAGWELKGTRRVNAAGELLDI
                     EFLIFEPTFERVIAPYLKNLHALGIQANIRRVDPAQYERRVKAFDFDIVTQRYVMRLT
                     PGVELFNFFGSGAADTEGSFNLAGIKDPVIDALVNKVMEAESRKELVTATRALDRVLR
                     AGHYWVPHWYKASHNIAHWNKFGRPEIKPKYDRGIVDTWWYDAEKAAKL"
     gene            complement(6428..8269)
                     /locus_tag="VF226_07490"
     CDS             complement(6428..8269)
                     /locus_tag="VF226_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7073869.1"
                     /translation="MALAAVALALNAGGADLRAGTETRHHALSLVGEPKFGPDFQQFD
                     WVNPDAPKGGTVRQWAMGTFDSLNAFSVQGNAALGLGLIYDTLMASSPDEPSTEYGLV
                     AEWVSFPDDFSSATFGLRPEARFHDGEPIKPEDVVFSLEALKKAHPHYNAYYRNVVRA
                     EKTGDHEVTFTFDQKGNRELPQIVGQLPVLPKHFWEGNGHNGSPRDLSRSTLEVPLGS
                     GPYKIKSFDPGRSIVYERVKDWWGKDLPVNKGQWNFDELVFTYFRDRVPAFEAFKVGS
                     IDFWRETSAKNWATSYDFDAVRRGLVKKEEIPIKRVTPMQAFVMNLRRPQFQDVRVRQ
                     AFNLAFNFEWMNKNLFFDQYKRTESFFGNSELEATGLPTGRELEILNEIKDQVPPEVF
                     TTEWKNPVNATPQDQRNNLRQAARLLAEAGWKPRNGILTNDKGEQLAVEFLLVQPDFE
                     RVVLPFKTDLERLGVKVAVRIVDASQYQRRVDTFDYDIIVDSFPQSISPGNEQRNFWG
                     SAAADIEGSQNSIGIKNPAIDKLIDKIIFAKDREELVAATRALDRVLLWNYYVIPQWH
                     TPYDRIAIWDVFGRPEKLPEQDPSFLRVWWLDPAKLQALNAKRGQQG"
     gene            complement(8628..9179)
                     /locus_tag="VF226_07495"
     CDS             complement(8628..9179)
                     /locus_tag="VF226_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX7073870.1"
                     /translation="MDTWEFNKIAGAVLAALLLMFGTATFIDIQMSHKPEKAGYTLPV
                     EITDAQGPAQPSAPAGVDVEKVKSLLQNASAENGQAAFKRCSTCHTTDKGGRNSTGPN
                     LWGIVGRARASHEGFNYSTAMKSKEGEWTYEELAHFIHNPKGYIPGNKMAFAGLKDEA
                     EIADLLAYLRTLNDNPPPLPTAN"
     gene            9357..10115
                     /locus_tag="VF226_07500"
     CDS             9357..10115
                     /locus_tag="VF226_07500"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041798121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7073871.1"
                     /translation="MTGTLVIIPARLQATRLPGKPLADIHGRPMIVHVWERAMAADVG
                     RVVVATDTREIADAIKSAGGEAVMTRPDHASGSDRIFEALQHADPNGKAAYIVNLQGD
                     LPTLEPRLVQECLAPLSTPGPDIATLAAEITEPEERVAPSVVKVVGTPIGSGAANRLR
                     ALYFTRATAPYGDGPLYHHIGIYAFRRSALERFVSLPPSPLEQREKLEQLRALENGLR
                     IDVALVDTVPLGVDTPADLERARQLLAPERTLKT"
     gene            10234..11091
                     /locus_tag="VF226_07505"
     CDS             10234..11091
                     /locus_tag="VF226_07505"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX7073872.1"
                     /translation="MSNRISYQGEPGANSHMACVEVFPDLEPLACPTFEDALAAVKAG
                     EARYAMIPIENSVAGRVADIHHLLPSAQLFIVGEHFLRVRHQLMALPGASLDTIKTVM
                     SHTQALGQCRNTLRRLGLKPVPEADTAGSARLVSQRNDLSLAAIASTLAAEIYGLKIL
                     KSDIEDEAHNTTRFVILAPEPDDAEPGQGPFVTTFIFRVRNVPAALYKALGGFATNGV
                     NMTKLESYQLNGAFTATMFYADIEGHPADRPVRLALEELSFFSSELKVLGTYRASPYR
                     KLVEQQRYG"
     gene            11125..11874
                     /locus_tag="VF226_07510"
     CDS             11125..11874
                     /locus_tag="VF226_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073873.1"
                     /translation="MKLKDKVAIVTGGASGFGKAIAETYAREGAKVMVADINGQGARE
                     VANSIGEAAAHIACDVSKKADVDAMVAETVKTFGGVDIMVNNAGYTHKNQWLLNVAEE
                     EFDRIFAVNVKAIYLATLACVPEMEKRGGGVIINTASTAGVRPRPGLTWYNGSKGAAI
                     TLTKSMAVELAPKKIRVCAINPVIGETGMLELFMGMPDTPENRAKFLATIPLGRMSKP
                     ADIANAALFLADPASEFITGVALEVDGGRCV"
     gene            complement(12025..12972)
                     /gene="nudC"
                     /locus_tag="VF226_07515"
     CDS             complement(12025..12972)
                     /gene="nudC"
                     /locus_tag="VF226_07515"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEX7073874.1"
                     /translation="MFKPTLGASTLLDRQTHKRSDAAYLEALLAAPEARFLVLADQKP
                     VIRSNPERTEGSIRWFTREEMQRVGLPVTDSLFLGVDRASGGGRFAIAVTEHRARNAP
                     GGLEMMRPIVDLRSLAMQGVLPPEELSLLGLAKALAAWHENTRCCGHCGGTTLTKDGG
                     WRRKCWACGQEHFPRTDPVVIMLVVDPPNDRCLLGHEARFAENMWSTLAGFIEPGEDI
                     EHAVRRETKEETSIDVGEVRFHSTQPWPFPHSLMIGCIGIAQSTDIKIDPNEIVDARW
                     FPREDVRLMLEGKHPEGLWVPGQQAIARSLITAFVEGEY"
     gene            13174..13269
                     /gene="ffs"
                     /locus_tag="VF226_07520"
     ncRNA           13174..13269
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF226_07520"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13411..15159
                     /locus_tag="VF226_07525"
     CDS             13411..15159
                     /locus_tag="VF226_07525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7073875.1"
                     /translation="MANNSTPPTDLLGGEGQSVTKPYVVLARKYRPETFEQLIGQEAM
                     VTTLKNAFATDRIAQGYMLTGVRGVGKTTTARILARALNYSKPGLPDRPTMDMDGYGE
                     HCLQIIQSRHPDVIEMDAASNTGVDNVREIIENARYRPILARYKVFIIDEVHMLSKSA
                     FNALLKTLEEPPEHVKFIFATTEVRKVPVTVLSRCQRFDLRRVDVPLLVKHFKSILAR
                     EGAGAEDEALALIARAAGGSVRDGLSILDQAIAMGSGTVTVPAVRAMLGLADRGRIFD
                     LLESLMRGAPADALRQLEQLHNDGADPAQVLADLADTVHAATRIKVVGEEGASEDLSA
                     EDRRRAAALGNQLSVPVLTRAWQMLQKGYEETSRSAQPLQAAEMVLIRIAHAAELPSP
                     DDLIRLLGNRGAPAQVSASAPAPAPQDGARMRASPSAVQPGREADADRPAAPVRPSAP
                     PAHDLHSFADVVALAGAHRDARLKVHLEEHVRLVRFEPGRLEIHPIEGSPPGLANELS
                     EKLAKWTGRRWVVAVSREEGEPPIGEVRRQRMAAELAEIKAHPAVQALLDVFPGAEVK
                     AVRPLTDPPQDESKAG"
     gene            15209..15529
                     /locus_tag="VF226_07530"
     CDS             15209..15529
                     /locus_tag="VF226_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7073876.1"
                     /translation="MKDLMGMMKQAQELQARMQKMQEELAALQVEGQAGAGLVRVTLN
                     GKGEMAALKIDPSLLKPEEVEILEDLIIAGFQDAKGKVEAAIQEKMQEVTGGLPLPPG
                     LKLF"
     gene            15561..16169
                     /gene="recR"
                     /locus_tag="VF226_07535"
     CDS             15561..16169
                     /gene="recR"
                     /locus_tag="VF226_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7073877.1"
                     /translation="MAGRIMAGPEIERLVQLLGRLPGLGYRSARKAALALLKRRDELL
                     VPLAAAMNAAAEKLVECSVCGNIDTIDPCTICQDPRRDRSLIVVVEDISDLWALERAG
                     VVNSVYHVLGGHLSPLDGIGPERLNIGRLIERAQHPEVKEVLLALNATVEGQSTAHYL
                     TEQLEGTAVSVSRLAQGVPVGGELDYLDEGTLAAAIKARRRV"
     gene            16263..17096
                     /locus_tag="VF226_07540"
     CDS             16263..17096
                     /locus_tag="VF226_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7073878.1"
                     /translation="MRHLTLRNGARVPALGLGTWGMGERRGSRSAEVKALQLGLDLGL
                     TLIDTAEMYGEGGAEEVVGDAIAGRRDEVYVVSKVYPHNASRSGAIAACERSLKRLRI
                     ETLDLYLLHWRGSIPLDQTVEAFEKLKADGKIRAWGVSNFDPSDMADLAKAAPQGACV
                     ANQVLYHLGSRGIEWDLLPECRQQGVAIMAYSPLGQGGILRNGRLKAVADKHGVSPAA
                     AALAWVLRHDHVFTIPKAASLEHVRANAAAADLVLDEEDLASLDKAFPPPSGPEPLAM
                     L"
     gene            17891..18109
                     /locus_tag="VF226_07545"
     CDS             17891..18109
                     /locus_tag="VF226_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073879.1"
                     /translation="MKHYERPVLVKMARLADITAGSDFFSPAVFTSEPPPPPPPPDET
                     PKDHDKDHDKDHHKDGGWKGGGDWKGKS"
     gene            18373..18666
                     /locus_tag="VF226_07550"
     CDS             18373..18666
                     /locus_tag="VF226_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073880.1"
                     /translation="MLKVLIPAAIASLIAITPASAMAPTQAGTQLKPLNVSDLVNVAS
                     RKRYKRHSHPKWRYVPGRRYSHAPRGWRRYHRRPYFWRTRGCIIVGPVWFCPY"
     gene            complement(18748..19758)
                     /locus_tag="VF226_07555"
     CDS             complement(18748..19758)
                     /locus_tag="VF226_07555"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HEX7073881.1"
                     /translation="MATHTFFCIDGHTCGNPVRVVAGGAPALSGKTMIEKRAHFLAEF
                     DWIRTALMFEPRGHDMMSGSILMPPTREDCDVAILFIETSGCLPMCGHGTIGTVTTAI
                     ERGLVQPRTPGQLRLDTPAGVVVAEYGQNGPFVEWVRITNVPSYLHASGLEADVPGIG
                     RLKVDVAYGGNFYAIVDPQETFRGLEHIQPSEVVALSPVVRRVFGEAFSFVHPEKPEI
                     KGLSHVMWTGKPVKPDSTARNAVFYGDKAIDRSPCGTGTSARMAQWAAQGRLSEGQEF
                     VHESIIGSQFIGRIAGRAKVGDYDAIIPSIQGWARITGFNTIFVDDRDPYWKGFQVVD
                     KE"
     gene            complement(19777..21009)
                     /locus_tag="VF226_07560"
     CDS             complement(19777..21009)
                     /locus_tag="VF226_07560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7073882.1"
                     /translation="MHVIIIGAGIIGLAAAHRLLDQGCAVTLVDRGEQTAASRGNAGI
                     IAHVDIQPLASPQMLLQAARWFIDPLGPLAVRPAYAAKLAPWLFRFLLASTPSRMQRS
                     KQGIVPLQMMALPAWERMLGRLNLKRELNRNGMLYVYENVDVFEAGKAGYREQVELGI
                     PIELIDGSVLRAMEPALSERFQRAAFFPSVAHVNDPYQLALKLASAAADRGAELLEGN
                     VSAIAIASGDEPVVVLEGGRTMTADRVVIAAGAWSKHLAACLGDKVPLDTERGYNATI
                     SKPGITLSRPIMFEEHAFVFTPLKTGLRIGGAVELASTSATPNWKRLEALLAKARAFA
                     PGLQEEGRVDYMGCRPSLPDSLPVISTSRWSSRIVYAFGHAHHGLTHAAATAELVGAL
                     ISGAQPAIDLSPYSAQRF"
     gene            21225..23030
                     /locus_tag="VF226_07565"
     CDS             21225..23030
                     /locus_tag="VF226_07565"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX7073883.1"
                     /translation="MANTTDGKRMKLRSQAWFDNPDNIDMTALYLERMTNYGLTIEEL
                     QSGKPIIGIAQSGSDITPCNRHHLELAKRVREGIREAGGIAFEFPVHPIQETLKRPTA
                     ALDRNLQYLSLVEVLYGYPIDGVVLTTGCDKTTPAQIMAAATVDIPAIVLSGGPMLNG
                     WYNGERTGSGTIVWLAREMLAKGEIDHKKFVELVASSAPSAGHCNTMGTATTMNSLAE
                     ALGMSLPGCAAIPAPHRDRGQMAYLTGKRAVEMVHEDLRPSKILTRAAFENAIVVNSA
                     IGGSTNAPIHLNAIARHVGVELDNDDWERIGYDIPLLVNLQPAGEYLGEDYYRAGGVP
                     AVVAELIEKGKIREDAITVNGKTLVENCKGRFSTDRRVIKPYDEPLKPHAGFLNLKGN
                     LFDSAIMKTSVIDEEFRKRYLSNPNDPNAFEGKAVVFDGPEDYHARIDDPALEIDENT
                     MLFMRGTGPIGYPGGAEVVNMRTPAYLLKKGIRALPCIGDGRQSGTSGSPSILNASPE
                     AANNGGLALLRTGDRVRIDLNKRTANILISDEELEQRRKELMAAGGYKYPESQTPWQE
                     IQRSLVNEMSEGAVLKNAVKYQRIAETKGIPRDNH"
     gene            complement(23341..24165)
                     /locus_tag="VF226_07570"
     CDS             complement(23341..24165)
                     /locus_tag="VF226_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7073884.1"
                     /translation="MPTISMQDRTQIYYKDWGSGQPVVFSHGWPLCSDAWEAQMMAVA
                     SNGFRAIGHDRRGHGRSSQPWTGNDMDTYADDLATLIEALDLRDVILVGHSTGGGEVA
                     RYIGRHGTERVAKVALVSAVPPLMLQTPDNPGGLPISVFDELRAASLANRSQFYRDLA
                     SGPFFGFNRPGAKVSQGSIDSFWLQGMMAGHKNAYDCIAAFSETDFTEDLKRFDVPTL
                     IVHGDDDQIVPIDAAGRASAKIVKHAELKVYPGGAHGLADTSREQLTSDLLAFISA"
     gene            complement(24278..26686)
                     /gene="lon"
                     /locus_tag="VF226_07575"
     CDS             complement(24278..26686)
                     /gene="lon"
                     /locus_tag="VF226_07575"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7073885.1"
                     /translation="MAEQEAPRRSKSGPELPSPPADALIILPVRDLVLFPKVVFPITI
                     GREKSVLAAQQAVREQRQIGVLTQRDPEVADPAPVDLYRMGTVADIVRFVTGTDGTHH
                     IVCQGQERFHVTEFLSGWPFFVARVLRIPDAETRTPEIEARFLHLQRQAIEALELLPQ
                     APKEMIATIQSVTSPGTLADLAVAYMDIPSSEKQEILETVNLAVRMEKVSKLLAHRIE
                     VLRLSQEISRQTKAALDQRQREVLLREQMAAIQRQLGEGEDSKAQEMAELEQAITKAE
                     MPPDVEEHARKELRRLSRMPEAAAEYGMVRTYLDWLVELPWKLPEEPPIDIEHARKIL
                     DEDHYGLEKVKRRIIEYLAVRKLAPQGKAPILCFVGPPGVGKTSLGQSIARAMGRKFQ
                     RVSLGGVHDEAEIRGHRRTYVGALPGNIIQAIRKAGSRNCVLMLDEIDKLGAGIHGDP
                     AAALLEVLDPEQNSTFRDNYLAVPFDLSRVVFITTANILDTIPGPLRDRMEVISLPGY
                     TAEEKLQIARRYLVRRQMEANGIKPEQVEITDAAINEIIEHYTREAGVRNLEREIGRA
                     LRHAAVRIAEGQTEQIRIDAGDLRAILGAPQFENEIAMRTSVPGVATGLAWTPVGGDI
                     LFIEATRIPGSGKLILTGHLGEVMRESAQAALSIVKNRADQLGIDPARFEKNDIHIHV
                     PAGAVPKDGPSAGVAIYVALVSLLTRRCVRSDTAMTGEISLRGLVLPVGGIKEKVVAA
                     ARAGITRVILPIRNRKDYEDIPEEARKELDFIWIERVEEALAASLAPKEEANAPRHAE
                     QAAE"
     gene            complement(26702..27112)
                     /locus_tag="VF226_07580"
     CDS             complement(26702..27112)
                     /locus_tag="VF226_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7073886.1"
                     /translation="MDKNFPSWMWSEACEALARAERLHHKLFDPQAGVGRQPSWRPPV
                     DVLETEDEVLVFIALPGVDPDEVQAVIENCDLVIAGARVLPAAFRTAAIHRLELPQGR
                     FERRIRLPEGSYRAIRWSASQGCLLIRLEKTAEY"
     gene            complement(27161..27352)
                     /locus_tag="VF226_07585"
     CDS             complement(27161..27352)
                     /locus_tag="VF226_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073887.1"
                     /translation="MPRKKPARESEEKEALRKSRRSKTFMHPAGPHARPELTDPEATP
                     GAGALPDISKDKDVDPGSG"
     gene            27690..27890
                     /locus_tag="VF226_07590"
     CDS             27690..27890
                     /locus_tag="VF226_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073888.1"
                     /translation="MDSRIWWLLGAVVIIVILVALLFPTGEPTTTATTPPATTAPEST
                     SPPAKTTPSTPEKTSPPAGQQQ"
     gene            complement(27933..28835)
                     /locus_tag="VF226_07595"
     CDS             complement(27933..28835)
                     /locus_tag="VF226_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7073889.1"
                     /translation="MLPRLAEKAFDNAYLLLSLMALFWAGNQVLGRAVAGHVPPVALS
                     FLRWSLATLILLPFALPHLRRDWPVVRQKWLFLGFLGVIGGGAFNTLQYIGLNYTTAL
                     NSLVLNSMGPIMIAIACVLLFKDRLTVTQMVGTVISLTGALVVVARGKPEVLATLSLN
                     RGDLMILFAMAITAVYTAYLRLRPEIHWITFLFTMFLASGLFNLPLVLIEMFVFGQFV
                     QPTLFTVLAIAYVSIFPSILSYLCFTRGVELIGGVRAGIFMHLIPLFGALMAIGLLGE
                     PLAAFHIVGFMLILTGVALTSRKP"
     gene            complement(28919..29848)
                     /locus_tag="VF226_07600"
     CDS             complement(28919..29848)
                     /locus_tag="VF226_07600"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7073890.1"
                     /translation="MQQTSREPFRGVFPIAPTPFTDQGSLDLDGQRRVLDCMIDQGVD
                     GICLLANYSEQFLLSDEERNTLLEVCLAHVAGRVPVIVTCSHFSTDIAAQRARAAAKA
                     GASMVMMMPPYHGATLKAGEKGILEHFQRVADAAGIPIMVQDAPLSGVELSVPFLTRL
                     AREVPLVRYFKIEVPGTAAKLRALIEAGGDAIAGPFDGEEAITLMADLDAGATGTMPS
                     ALLPDLIKPVLDHHRAGRRAEAMDAYARILPLINFENRQCGLRATKTVMQAGGVIKSD
                     AVRHPLEPLHPATRAQLLELAEQVSPLALRWGR"
     gene            30062..32059
                     /locus_tag="VF226_07605"
     CDS             30062..32059
                     /locus_tag="VF226_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HEX7073891.1"
                     /translation="MAGANKSEGGQQVQRDKPWLFRTYAGHSTAAESNALYRRNLAKG
                     QTGLSIAFDLPTQTGYDSDHELAAGEVGKVGVPISHIGDMRTLLDGIPLDRMNTSMTI
                     NATAAWLFALYIAVADEQGAPRDKLTGTIQNDIIKEYLSRGTYVFPPEPSLRLTRDTI
                     VFSCREVPKWNPINVCSYHLQEAGATPVQELAFALATAIAILDSVRDSGAVDREAFEA
                     VVGRVSFFVNAGLRFITEMCKMRAFVDLWDEITRERYGVTNPKHRRFRYGVQVNSLGL
                     TEQQPENNVYRILLEMLAVTLSKNARARAVQLPAWNEALGLPRPWDQQWSLRMQQVLA
                     YETDLLEYGDIFDGSVEIERKVEELKRQAREELATIERLGGAVAAIDYMKRRLVESNA
                     ARLARIESGEQVVVGVNRFTETEPSPLSAGEGAVQTVGPEVEVEQIARLKAWCEARDE
                     AAVARALADLAQAAKEDRNIMEPSIACAKAGVTTGEWGMTLRRIFGEYRAPTGVGQAA
                     GEGVVGLEALREQVRAAAAQLGRPIKLLVGKPGLDGHSNGAEQIAVRARDCGMEVVYE
                     GIRLTPKQIVRAALEESVHVVGLSVLSGSHVPLVRAVLDGMRAENMDDIPVVVGGIIP
                     PEDAASLKAMGVARVYTPKDFQLNDIMSDIVQLVVRQPAAA"
     gene            32067..32417
                     /locus_tag="VF226_07610"
     CDS             32067..32417
                     /locus_tag="VF226_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073892.1"
                     /translation="MAKISEETRRKLDRILLMERLKWLAAAVIAIAAIVGFFILIDLD
                     AEVVDYQVAGKVEHVGIANLKTVRQAVEVDVALDDGRHARVIAAKEHEPHVGDRVRIV
                     EHKHATGRVTFTWR"
     gene            complement(32449..33492)
                     /locus_tag="VF226_07615"
     CDS             complement(32449..33492)
                     /locus_tag="VF226_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7073893.1"
                     /translation="MADPPRPDDLPDSEVRLVRRILILAAFAVLAAAIWFLSDVLLLT
                     FAGVLLAVLVRSISTPLKTKLRVSEGVALSLSGAVILVVLVSGLALFGAQIAGQFNQL
                     LTVLPEAANRILAEFGVTRVQDMLTGTAVGNVLTTAYEWTTAIVSIATALVFVIFTGI
                     YLALDPIPYRNGFILLFPPRVQPLIEETVDDAGRALSLWIGGQLIAMLMVGVMTALGL
                     WLIGLNSALALGFIAGLADFVPIVGPITAAIPGLLVASGQGFDMVLWTLAVYVIVQQI
                     ESNIIVPIVVGNAVKIPPALGLFAVIALGVLFGPLGVLFAYPLAVVADVAVRRLYIRE
                     TLGEKVEISGEEK"
     gene            33701..33787
                     /locus_tag="VF226_07620"
     tRNA            33701..33787
                     /locus_tag="VF226_07620"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33735..33737,aa:Leu,seq:caa)
     gene            complement(33883..>33999)
                     /locus_tag="VF226_07625"
     CDS             complement(33883..>33999)
                     /locus_tag="VF226_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX7073894.1"
                     /translation="VVDASVMPLITSANTNAASLMIGEKGAALIKGEARAPS"
BASE COUNT         6168 a        10808 c        11066 g         5957 t
ORIGIN      
        1 ttgcgctcgc tgcggctcca gccacggagc tggaacccga gcgccttcag cgccttcgcc
       61 gtcgcctctc ccatgacgcc aagtcccatg aggccgacgc gaacctcgtg cgtcccaggc
      121 tccggccgga actcccactt acgctcgcgc tgcaactcga ggaactcgca catgcggcgc
      181 tgatggaaca gcacgtggag cacgagatag ctgaccatcc ggttcgtgag gtccgggtcg
      241 acgtagcgca cgatgggcac atccggcagc tcggggtcct tcatcaggtg atccacaccg
      301 gcgccaacgg agatgatgag ttcaagattg gggaaggtct tgagcacgcc aaccggcggg
      361 ccccaggcaa gcgcgtagcg gatgtcctcg atgcgccccg tctcgggcca tacccggatg
      421 tcgagaccag agttcagggc ccgcgccgct gccgcaaact cctccgaccg ctgacttcca
      481 atgatgagca gcgccatcag ctcccccgtt cgattgggtt aaacggcgag cgctccgcga
      541 tgctcgacct tgaggtcgaa tgcggccgcg agcagggcct tcgtgtagtc ggtcttcggc
      601 gcgcggaaga tctcctccgc agggccttcc tccaccacct tgccgttccg catcacgatg
      661 acgtagctcg acagcgcccg cacgaccttg aggtcgtgac tgatgaacag gtaggccaga
      721 ttgtgccgct gctgcaagtc ccgaagcaga tcaacgattt gcgcctgcac ggacatgtcg
      781 agggcgctcg tcggctcgtc cagcatgacg aacttcggct cgagcaccat ggcccgcgct
      841 atggcaatgc gctggcgctg accgccggag aactcgtgcg ggtagcggtc ctgcgcctcg
      901 gggtcgagcc cgacctcgct caatgcctgt gccacccggg cgcggcgctc ttcgtagctc
      961 aggtgcggct gctggatgag gaggccttcc tcgatgatct gcccgacgga caggcgcggc
     1021 gacagcgacc cgtaggggtc ctggaagacg atctgcatct ccttgcgcag cggccgcatg
     1081 cgcttggagt tcagtccgtc gatgcgattg ccgagatagg ccacctgtcc cttggaggag
     1141 atgagccgca tgatggcgag gcccagcgtg gtcttgcccg agccggattc gccgaccacg
     1201 cccaaggtct ggccggcgcg cagcttcagc gacagtccgt ccactgcctt cacatggtcg
     1261 accgtccgtc gcagcaggcc cttgcgaatg gggaaccaga ctttcagatc gtccgtttgg
     1321 accacgatgg gggcagacgg gttcatcggg ggcggctcgc ccttcggctc ggcgctgatg
     1381 aggtggcgcg tgtaggggtg cttcgggtcg gcgaagatgt ccgccgtctt gccttcctcc
     1441 accaccttgc cgttgttcat cacgtagacg cgctcggcca tcttccgcac gatcccgagg
     1501 tcgtgcgtga tgagcagcat ggccatgccg agctcggact gcagctcctt taggagttcc
     1561 aggatctgcg cctggatggt gacgtcgagc gcggtcgtcg gctcgtcggc gatgagcagg
     1621 tccggctcgt tggcgagcgc catggcgatc atcacgcgct ggcgctgacc accggagagc
     1681 tggtgcggga aggccgtcag ccgcttctcg ggatcgcgca ggccgacgcg gcgtaaaagc
     1741 tccagcacgc gctggcggat cgccttgtcg tccatgccgc ggtgcagctt caacacctcg
     1801 cccacctgct tttcgatggt gtgcagcggg ttgagggagg tcatcggctc ctggaagatg
     1861 atcgatatgc gcgcaccgcg aatgtcgcgc atatcggctt cggaagcctt cagcaggtct
     1921 ttgccattgt agatgatctc gcccgatggg tgcgaggccg cggggtaggg cagcagccgc
     1981 atgatcgaca gcgccgacac ggtcttgccg gagcccgact cgccgacgag cgccaccgtc
     2041 tcgcccttgt tcacctcgaa cgagacgcct ttgacggcga ggtttgtcgt gtcgcccgat
     2101 cggaacgcga cggaaaggtc gcggacctgg acgagggggg caccagtctt cattctgacc
     2161 tcaccggaat gtcttgcgcg gatcgagcgc gtcgcgcacg gcctcgccga tgaagatgag
     2221 caggctcaac aggactgcga gcgagaagaa ggcggtgagc ccaagccacg gtgcctgcag
     2281 gttggccttg ccttgcgcca ggagctcacc gagagaggcc gaacccggag gcaggcccag
     2341 cccgaggaag tcgagcgcag tcaacgcggt gatgctgccc gagagattga agggcaggaa
     2401 cgtcagcatc gccaccatgg cattcggcag caggtgcttg aacatgatct tgcggtcgga
     2461 gaggccaagg gctcgcgctg ccgtcacgta ttcgaaattg cgggcccgca ggaattccgc
     2521 gcgcacgacg cccaccagcg atacccactg gaacagcagc agcacgccga gcagcgtcca
     2581 gaacccgggg accagcacag aggagatgat gataagcacg tagagcgacg ggatcgacga
     2641 ccagatctcc agcacacgct ggaagaatag gtcgacgcgg ccaccgaagt agccctgcac
     2701 cgcacccgcg gtgacgccga tgacggagga gaagaacgtc agcagcagac cgaacagcac
     2761 cgagatccgg aaaccgtaga tcatgcgggc gagcacgtcg cgaccctggt cgtctgttcc
     2821 gagccagttg cggttgccga gctcctggaa tcgcgcgagc tggtccgccg gcgcctcgca
     2881 cagctgcgca ttcgttgccc agagcggcgg ggcagggaag ccgaggcagc ggtcctcggg
     2941 tcccttgatg cgcggatagt ccttgttgat cgaggtgtag gagtagcgga tcggcggcca
     3001 gatcatccag ccgttgctct cgatctcgtc gatgatgaac gggtctttat agtccgtcgt
     3061 cgccaggaag ccgccgaact tcgactccgg gtagtcgacc agaacgggcg agagcagctc
     3121 tcccttgtag tagacgagca gcggcttgtc gttggcgatc agctccgcgc cgaggctcag
     3181 cacgaagagc acgaggaaaa tccagaagct ccagtagccg cgccggttgg ccttgaagtt
     3241 ctcccagcgg cgccggttga tcggcgacag cttgaagcgc ccggcggcac cgcgcttgcg
     3301 gccgatccac gttgtcggct ggcgctggaa acccggcgtc gtggcgatct tctcctgcgt
     3361 ctcgacatcg gctgccgtat cgctgggcgc gatcggcttc agcttgtcgt ctgtgcggat
     3421 atccatcggc tcagacctcc cggctctcga agtcgatccg cggatcgatc agtgtgtaga
     3481 cgaagtccga gatcaggttc accaccagac cgacgagcga gaagatatag agcgttgcga
     3541 atacgaccgg atagtcgcgg ttgatgatgc tttcgaagcc gagcagcccg aggccatcca
     3601 gcgaaaagat ggtttcgatc agcagcgagc cgctgaagaa ggcggagatg aatgcgccgg
     3661 ggaagccggc gatgatgagc agcatcgcgt tgcggaacac gtgcccgtag agcacctgtc
     3721 tttcgttcag tcctttggcc cgcgccgtga tgacgtattg cttccggatt tcgtcgagga
     3781 agcagttctt ggtgagaaag gtcatcgtcg tgaaggcgcc gagcgccatc gcggtgatcg
     3841 gcagcgccaa gtgccagaag tagtcggcga tctttccggc caacgtcagg tcttcccagt
     3901 agtcggaggt gagcccgcgg gacgggaacc actggaagaa ggagccgccg gcgaacagga
     3961 cgagcagcag aaccgcgacg agaaatcccg ggaccgcgta gccgaccacc aggaatccac
     4021 tggtccagac gtcgaagcgc tcgccgtcgc gcacggcctt gcggatgccg agcgggatgg
     4081 aaatgagata ggtcagcagc gtcatccaca ggccgagcga gatcgaaacc ggcagcttct
     4141 cggcaatgag ctggatgacg ctgacgtcac ggaagtagct cttgccgaag tcgaacatga
     4201 ggaagctctt catcatgatg aagaaccgct cgtgcgccgg cttgtcgaag ccgaactgct
     4261 tctcgagctg cttgatgaac tccggatcga gcccctgggc gccgcggtat ttcgagccgc
     4321 cgccctcgtg ccccatctgc tgggctgcct gaccggccag cccatccccc tggctgccgc
     4381 cgatgcgaga tgtcgccgac acgtcggtgc cggagatctg cgcgatcacc cgctcaaccg
     4441 gtccgccagg tgcgaactgg atgatggcga agctgatcag catgatcccg agaagtgtcg
     4501 ggaagatcag gagaattctc ttgagaagat atgcgcccat gcggggtttg aagctccctt
     4561 ccgctcttgt ggcgtagtaa tacctaaagc ttcgcggctt tctccgcgtc gtaccaccac
     4621 gtgtctacta tgccgcggtc gtatttcggc ttgatttccg gcctgccaaa cttgttccag
     4681 tgcgcaatat tgtgggatgc cttgtaccag tgcggcaccc agtagtgccc ggcacgcagc
     4741 acgcggtcga gcgcccgcgt cgccgtcacc agctccttgc gcgactcggc ctccatcacc
     4801 ttgttcacca gcgcatcgat gaccggatcc ttgatccccg ccagattgaa gctgccctcc
     4861 gtatcggccg ccccgctgcc gaagaagttg aacagctcga cgccgggcgt cagccgcatg
     4921 acatagcgct gggtcacgat gtcgaagtcg aacgccttca cccggcgctc gtactgcgcc
     4981 gggtcgacgc ggcggatgtt tgcctgaatg ccgagcgcat gcaggttctt gaggtagggt
     5041 gcgatcaccc gctcgaacgt cggctcgaag atgaggaact cgatgtcgag cagctcgccg
     5101 gctgcattga cgcgccgggt gcccttgagc tcccaaccgg cttgcgtaag aagctgtgcg
     5161 gccgtcctca acaggcgccg gtcagagcct gacccgtcgg aaacgggcgg cgtgtacggc
     5221 tcgccgaaaa cctcgggcgg caaccgatcg cgatagggct cgagcagggc cagctcttcc
     5281 ggcgagggct tgccctcagc cttgagatcc gagttctcga agtagctcgc cgtgcgcttg
     5341 taaagtgaat agaacaggtt gctgttcgtc cactcgaagt cgaaggcgta atcaagtgcc
     5401 ttgcggaccc gcggatccga gaacttcgcg cgccgcgtgt tgatgaagaa gccctgggcg
     5461 cccgatgggc tctcgtcggg caaggtcagc agcacgatgc ggccgtcctt gacctgcggg
     5521 atgtcatagc ccgtcgccca gtccttggag gtgaactcct cgcgcaggtc gtaggcgccc
     5581 gccttcaggc tttccagctg cgcggtacgg tcgcggaaat actcgtagcg gacctcgtcg
     5641 aaattgtacc ggccgcggtt caccggcagg tccttggccc agtaatcctc acggcgcttg
     5701 aaggtggtga agctgccctg cttgaactcg gagatgcggt acggaccgga ccccagcggc
     5761 ggctcgagcg tcgtctggtc gaactcgtgt ctcgcataat aggcctttga gagtacgggc
     5821 agctccgcca cgatcagcgg cagatcgcgc aggttctcgc cttcgaacac gtagcggacc
     5881 ttgtgcggct ccagcacctc tgcgcgaacg acgttgcgca gcgagaggcg gaactgcggg
     5941 tgacccttgg tcttcagcgt ctcgaaagtg aacgccacgt cctcggccgt cacgggcgag
     6001 ccgtcggcaa attttgcttc ggggcgaagc tcgaatgtga cggagcggcg gtcggcggcg
     6061 acggtcgcgg attttgcgat caggccataa acggcatccg gctcgtccat tgcgcgcacc
     6121 atgaggctgt cgaacagcag gtcgagcccc tgtgccgcgt caccgcgcag gatgaacggg
     6181 ttgaagctgt cgaaagtgat cgtgcccgcc ggaccgatca tcgacagccg tccgcctttc
     6241 ggcgcgtccg gattgacgta ctcgaaatgc gtgaagtccg gcccgtattt cagctcgccg
     6301 aaggcagaaa ggccatggct cggcgcctcg tcggccgaga ccggcatcac cgcggcgagg
     6361 gctaggagga tacttgccgc aagcggcctg acccatcgga gtccaggcgt ggcctcgcgg
     6421 gcgtaagtca tccctgctgc ccccgcttcg cgttcaacgc ctgcaacttt gccggatcga
     6481 gccaccagac ccgcaggaac gacggatcct gctccggcag cttctccggc cggccgaaca
     6541 cgtcccagat ggcgatgcgg tcatagggcg tatgccactg cgggatgacg tagtagttcc
     6601 acagcagcac gcggtcgagc gcccgggtcg ccgcgacgag ctcctcccgg tctttcgcga
     6661 aaatgatctt atcgatcagc ttgtcgatcg ccgggttttt gatgccgatg gaattctggc
     6721 tgccctcgat atccgccgcg gccgagcccc agaaattgcg ctgctcgttg ccgggcgaaa
     6781 tcgactgcgg gaacgagtcg acgatgatgt catagtcgaa cgtgtccacg cgcctctgat
     6841 actgcgatgc gtcgacgatc cggaccgcaa ccttgacgcc gagccgttcc aggtcggtct
     6901 tgaacggcag gaccacccgc tcgaagtccg gctgcaccag caggaactcg acggcaagct
     6961 gctcgccctt gtcgttcgtc agtatgccgt tgcgcggctt ccagcccgct tccgcaagga
     7021 gccgagcggc ctgcctcagg ttgttgcgct gatcctgcgg cgtggcgttc accgggttct
     7081 tccactcggt cgtaaagacc tcgggcggca cctgatcctt gatctcgttc aggatctcga
     7141 gctcgcggcc ggtcggcagg cccgtcgcct ccagctccga gttcccgaaa aagctctccg
     7201 tgcgcttata ctgatcgaag aacaggtttt tgttcatcca ctcgaagttg aacgccagat
     7261 tgaaggcctg gcgcacgcgc acgtcctgga actgcggccg gcgcaggttc atcacgaagg
     7321 cctgcatggg cgtcacgcgc ttgatcggaa tctcctcctt cttcacgagc ccgcggcgca
     7381 cggcgtcaaa atcgtaggag gtcgcccagt tcttcgcgct cgtctcgcgc cagaagtcga
     7441 tcgagcccac cttgaaggct tcgaacgccg gcacgcgatc gcggaagtag gtgaagacga
     7501 gctcgtcgaa gttccactgg cccttgttca ccggcagatc cttgccccac cagtccttca
     7561 cccgctcgta gacgatgctg cgccctggat cgaacgactt gatcttgtag ggaccggagc
     7621 cgaggggcac ctcgagcgtg ctgcgcgaaa gatcacgcgg cgagccgttg tgcccgttcc
     7681 cctcccagaa atgcttcggc agaaccggaa gctggccgac gatttgcggc agctcgcggt
     7741 tgcccttctg atcgaaggtg aaggtgacct cgtgatcgcc cgtcttctcg gcgcgcacga
     7801 cgttgcggta gtaggcgtta tagtgcggat gggccttctt cagcgcttcc agcgagaaga
     7861 ccacgtcctc cggcttgatc ggctcgccgt catggaagcg cgcttcgggg cgcaacccaa
     7921 atgtggcgct cgagaaatcg tcggggaagg agacccactc ggccacgagc ccgtattcgg
     7981 tcgagggctc gtcggggctc gaggccatca gcgtgtcgta gatcaggccg aggcccagcg
     8041 cggcgttccc ctgaaccgag aatgcgttca ggctgtcgaa cgtgcccatc gcccactggc
     8101 gtacggttcc acccttgggc gcatccggat tgacccagtc gaactgctgg aaatcgggcc
     8161 cgaacttcgg ctccccaacg agcgacagcg cgtgatgccg cgtttccgta ccggcccgca
     8221 gatcggcgcc gcctgcattg agcgccagcg ccaccgcggc cagcgccaag attcctttgc
     8281 ctatgctacg cgtgaaccgt gcccgcacac gttactcccg gttgagtgtt cggctgaggt
     8341 gccccccgac ccgtgtccat cctcggagcc cgtggaattg gacagagaaa tcgccggctc
     8401 cccggctccg aggcaatcgt gcagccgtta tagcagcaaa cgcgctcccg ccaacgcagt
     8461 ggcgggggcc gtcttaacaa agactttcat aacaggggcc taatccctga gccaactgcc
     8521 cacgggtcgc atacctggct gcgccagcga tttgcggcgg ctggtcacgg gtccgccata
     8581 ccagccaccg ggccgccagt ctcgcacttg aatgctgcgc cgccgcctca atttgcagtg
     8641 ggcagaggcg gcggattgtc gttgagggtc cgcaggtacg cgagcaggtc cgcgatctca
     8701 gcctcgtcct tgaggccggc gaacgccatc ttgttacccg ggatatagcc cttcggattg
     8761 tggatgaagt gggcgagctc ctcgtaggtc cactcacctt ccttggattt catcgcggtg
     8821 gagtagttga agccttcgtg cgacgccctc gcgcgaccga cgattcccca gaggttggga
     8881 ccggtcgagt tccgtccgcc cttgtcggtc gtatgacagg tgctgcagcg cttgaatgca
     8941 gcttgcccgt tttcggcgct ggcattctgc agcaggctct tgaccttctc gacgtccaca
     9001 ccggccggag ccgacggctg cgccggaccc tgggcgtctg tgatctcgac gggcaatgtg
     9061 tagccggcct tttccggctt gtggctcatc tggatgtcga tgaacgtcgc cgttccgaac
     9121 atgagaagaa gcgccgcaag cacagccccg gcaatcttgt tgaactccca ggtatccatc
     9181 gatccccagc cctcttgccc gcagcccgtc cgcgcttttc gtactcgtac cctggtcggc
     9241 ggccgactat agaaagtctc gaccttcatg caaccagccc agcggttcgg tccagtcgct
     9301 caccccgaga ccaattgtca ctcgttctaa atcactgtca gagaaaggcg cgcttcatga
     9361 ccggcacgct cgtcatcatc cccgcccgct tgcaggcgac ccggctgccc ggaaagccct
     9421 tggcggacat tcacggcaga ccgatgatcg tccacgtctg ggagcgcgcc atggcggccg
     9481 acgtgggccg ggtggtggtc gcgaccgaca cccgcgaaat cgcagatgca ataaagtcgg
     9541 cgggcggaga agccgtcatg acgcgcccgg atcatgcctc gggctccgac cggatcttcg
     9601 aggcgcttca gcatgccgat ccgaatggca aagctgccta tatcgtgaac ctgcaaggcg
     9661 atctgccgac cctggagccc cgtctggtgc aggagtgcct ggctccactc tcaactccag
     9721 ggcccgacat cgcgacactt gcggccgaga tcaccgagcc ggaagaacgg gtggcgccga
     9781 gcgtcgtgaa ggtcgtcggg acgccgatcg gcagcggcgc cgcgaaccgc ctgagagccc
     9841 tctacttcac gcgcgcgacg gcgccttatg gcgacgggcc gctctatcat cacatcggca
     9901 tctacgcatt ccgccggtcc gcgctggagc ggttcgtgtc gctgccgccg tcgccgctgg
     9961 agcaacgcga gaagctcgag cagctgcggg cgctcgagaa cggcctgcgc atcgacgtgg
    10021 cactcgttga cacggttccg ctcggtgtcg atactccggc cgatctggag agggctcgcc
    10081 aactgcttgc gcctgaacgc acgcttaaga cctgagcagg ggtctgttct ctcccattca
    10141 ccccgatcga acatcatgca gcagtccagg acgaaatcga tggcgggtgc cagccgctcc
    10201 gcgccccaat cggctcacag ccggagcaag gccctgtcca accgcatctc ctatcaggga
    10261 gagcccggcg ccaactcaca catggcgtgc gtggaagtgt ttccagatct cgagccgttg
    10321 gcgtgcccga cgttcgagga cgcgctggcc gcggtgaagg cgggcgaggc acgctatgcg
    10381 atgatcccga tcgagaactc ggtcgcgggt cgcgtcgccg atatccacca cctgctgccg
    10441 agcgcacaac tgttcatcgt cggcgagcat ttcctgcgcg tgcgccacca attaatggcg
    10501 ctgccggggg ccagcctcga tacgatcaaa acggtgatga gccatacgca ggcgctgggg
    10561 caatgccgca acacgctgcg ccgcctcggc ctgaagccgg tgccggaagc ggatacggcc
    10621 ggctctgccc gccttgtcag ccaacgaaac gacctgagtc ttgccgccat cgcctcgacg
    10681 ttggctgcgg agatctacgg cctgaaaatc ctcaagagcg acatcgagga cgaggcgcat
    10741 aacacgacgc ggttcgtcat cctggctcct gagcccgacg acgccgagcc cggccagggt
    10801 ccgttcgtga cgaccttcat cttccgggtc cggaacgtcc cggcggccct ctacaaggcg
    10861 ctgggcggtt ttgcgaccaa cggcgtcaac atgacgaagc tggaatccta tcagctcaac
    10921 ggcgcgttca cggccaccat gttctacgcc gacatcgaag gccatccggc agatcgcccc
    10981 gtgcggctgg cgctggaaga gctgtcgttc ttctcgtcag agctcaaggt tctcggtacc
    11041 tatcgcgcga gtccctaccg gaagctcgtc gagcagcagc gctatggcta gcccggacat
    11101 tcgggccgaa ctgcggagga catgatgaag ctcaaggaca aggttgccat cgtcaccggc
    11161 ggtgcgtcgg ggttcggcaa agccatcgcc gagacctatg cgcgggaagg cgccaaggtg
    11221 atggtggcgg acatcaatgg ccagggcgcg cgggaggtgg cgaacagcat cggtgaagcg
    11281 gcggcgcata tcgcctgcga cgtgtcgaag aaggccgatg tcgatgccat ggtcgccgaa
    11341 acagtgaaaa ccttcggcgg cgtcgacatc atggtcaaca acgccggcta cacgcacaag
    11401 aaccagtggc tgctgaatgt tgccgaggag gagttcgatc gcattttcgc cgtcaacgtc
    11461 aaggcgatct acctcgcgac cctggcctgc gtgccggaga tggagaagcg cggcgggggc
    11521 gtgatcatca acacggcttc cactgccggc gtcaggcctc gtccgggtct cacctggtac
    11581 aacggctcga agggcgccgc gatcactctg accaagtcga tggccgtcga gctggcgccg
    11641 aagaagattc gtgtgtgcgc catcaacccg gtgatcggcg agaccggcat gctcgagctg
    11701 ttcatgggca tgcccgacac gccggagaac cgggcgaagt tcctcgcgac cattccgctc
    11761 ggccgcatgt cgaagccggc cgacatcgcc aacgccgcgc tgttcctcgc cgacccggcc
    11821 tccgagttca tcaccggcgt ggcgctggag gtggacggcg ggcgctgcgt ttgaggcgtt
    11881 agcgcgccag atcatcaagg acgtcatccc ggtgcttgtt accgggatct acctcgacac
    11941 tcgctctggc gtctggggtt ggttggatct cgggcacaag gcccgggatg actctgggag
    12001 tagcagggag tagaagaccc ctcgtcagta ttccccctcc acgaaggccg tgatcagcga
    12061 tcgcgcgatg gcctgttgac cgggcaccca gaggccttcg gggtgcttgc cttccagcat
    12121 cagccggacg tcctcccgcg gaaaccagcg ggcgtcgacg atctcgttcg gatcgatctt
    12181 gatgtcggtc gactgggcta ttccgatgca gccgatcatc agcgagtgag ggaaaggcca
    12241 cggctgcgtc gagtggaagc ggacctcgcc gacgtcgatg ctcgtctctt ccttggtctc
    12301 gcgccggacg gcatgctcga tgtcctcgcc cggctctatg aagccggcca gcgtcgacca
    12361 catgttttcc gcaaagcgcg cctcgtgacc gagcaggcag cggtcgttcg gcgggtcgac
    12421 gacgagcatg atgacgacgg ggtcggtgcg cgggaaatgc tcctgcccgc aggcccagca
    12481 tttccggcgc cagccaccat ccttggtgag cgtcgttccg ccacagtggc cgcagcagcg
    12541 cgtgttctcg tgccaggcgg caagcgcctt cgcgaggccg agcaacgaca gctcctccgg
    12601 cggcagcacg ccctgcatgg cgagtgagcg caggtccacg atcggccgca tcatctccag
    12661 tccacccggc gcattgcgcg cccggtgctc ggtgacggca atggcgaagc ggccgccgcc
    12721 gctcgcacga tcgacgccca gaaaaagcga gtcggtgacc ggcagcccga cccgctgcat
    12781 ctcctcccgc gtgaaccagc ggatgctgcc ttcggtgcgc tccggattcg accggatcac
    12841 cggcttctgg tcggccagca cgagaaagcg ggcctcgggc gctgccagta gcgcctcgag
    12901 gtacgccgca tcagaccgct tgtgggtctg acggtcgagc agcgtcgagg ctccaagtgt
    12961 gggtttgaac atcctgcaat tcctcccagc ggacccgcac atcagcacag ccgcccgagt
    13021 ctgcgcaatg cgttccgggc atggctggta attatgtagg gagtcgggag caggcgcgcg
    13081 cgggtgccct ggccagaaag taacgggtcc tgcgtcgctc ctctccgggc gcaagacttg
    13141 taacttgccg gtcgagacgg catattccgc gcgggaggct ggcggcggac gtttccctcg
    13201 caaaccgggt caggtccgga aggaagcagc cctagcgatc aggcgacggg tcgttcgtca
    13261 gtctctcgtg ttctcgccgc ctctggtcgg gcctggggcg gccgctcgcg tcggcatgga
    13321 attcgtgtgc cgctggtcgc ggccggcgca tggagccgtc cttatttcca gtccaggggc
    13381 gacgagttcc taaggacaat tcaggggccg atggcgaaca attccacccc acccacggat
    13441 ctgcttggcg gcgagggcca gtccgtcacg aagccctacg tcgtgctcgc ccgcaagtac
    13501 cgccccgaga ccttcgagca gctcatcggc caggaagcga tggtgacgac gctcaagaac
    13561 gcgttcgcca ccgaccggat cgcgcaaggg tacatgctga cgggcgtccg cggcgtcggc
    13621 aagaccacca ccgcacgcat cctggcgcgc gccctcaact actccaagcc tggcctgccg
    13681 gaccggccga ccatggacat ggacggctac ggcgagcact gcctgcaaat catccagagc
    13741 cgccaccccg acgtgatcga gatggacgcc gcgtccaaca ccggcgtcga caacgtgcgc
    13801 gagatcatcg agaacgcccg ctaccggccg atcctggcgc gctacaaggt cttcatcatc
    13861 gacgaagtcc acatgctttc aaagagcgcc ttcaacgctc ttctgaagac gctcgaggag
    13921 ccgccggagc acgtcaagtt catcttcgcg acgaccgagg tgcgcaaggt gccggtgacg
    13981 gtgctgtcgc ggtgccagcg gttcgatctg cgccgggtcg atgtgccgct gctggtcaag
    14041 cacttcaaat ctatcctcgc ccgggaaggg gccggggctg aggacgaggc attggccctg
    14101 attgcgcgcg cggccggggg ctcggtgcgt gatggcctct ccatcctcga ccaggcgatc
    14161 gcgatgggct ccggcacggt gaccgttcct gccgtgcgcg cgatgctggg cctcgcggac
    14221 cgcgggcgta tcttcgacct gctggagtcg ctgatgcgcg gcgcgccggc cgatgcactg
    14281 cgtcagctcg agcagttgca caatgacggc gccgatccgg cgcaggttct ggccgatctc
    14341 gccgacacgg tgcacgcagc gacacgcatc aaggtggtcg gcgaggaggg cgcaagcgag
    14401 gatctttccg ccgaggaccg gcgccgggcc gcagcgcttg gcaatcaact ctccgtgccg
    14461 gtgctgacgc gcgcctggca gatgctgcag aagggttacg aggagacctc gcgctccgcc
    14521 cagccgttgc aggcggccga gatggttctg atccgcatcg cgcacgcggc ggagctgccg
    14581 tctcccgacg atctgatccg cctgctcggc aatcgaggcg cgccagcgca ggtcagcgcg
    14641 agcgcgccgg caccggctcc gcaggacggt gcccgcatga gagcctcccc ttccgcagtc
    14701 cagccggggc gcgaagccga tgccgatcga ccggctgctc cggttagacc atcggcgccg
    14761 cccgcgcatg atcttcacag ctttgccgat gtggtcgcac tggccggggc gcaccgtgat
    14821 gcccgtctca aggtgcatct cgaggagcac gtgcggcttg tccggttcga gccggggcgg
    14881 ctggagattc acccgatcga gggcagcccg ccggggcttg ccaacgagct gagcgagaag
    14941 ctggcgaagt ggaccggccg gcgctgggtc gtggcggtca gccgggaaga gggcgagccg
    15001 ccgatcggtg aagtgcgccg gcagcgcatg gcggccgagc tggcggaaat caaggctcat
    15061 ccggccgtgc aggctttgtt ggacgttttc cccggtgcgg aagttaaggc tgtacggccg
    15121 ctgaccgatc caccgcagga cgagagcaag gctggctgac cgttcaggcc gccggatcgg
    15181 cccccgtgcc ccacaagcgg agaaagagat gaaagatctg atgggaatga tgaagcaggc
    15241 gcaggagctg caggcccgca tgcagaagat gcaggaggag cttgcggcac tgcaggttga
    15301 aggacaggcc ggggccggcc tcgtccgggt caccctcaac ggcaagggcg agatggcggc
    15361 ccttaagatc gaccccagcc tgctgaagcc ggaggaggtc gagatcctgg aggatctgat
    15421 catcgccggt ttccaggatg ccaagggcaa ggtcgaagcg gcgatccagg agaagatgca
    15481 ggaagtgacc ggcgggctgc cgctgccgcc tgggctgaag ctcttctgac cgcgcagggc
    15541 tctcgggggc gagcgggaac atggcgggca ggatcatggc cgggccggag atcgagcgat
    15601 tggtgcagct cctcggacgt ttgcccgggc tcggctatcg ctccgcacgg aaggccgcgc
    15661 tggcgctcct gaagcgccgt gacgagctcc tggtgcccct tgcggccgca atgaacgcgg
    15721 ctgccgaaaa gctcgtcgaa tgctccgtct gcggcaacat cgataccatc gatccgtgca
    15781 ccatttgcca ggacccgcgc cgcgaccgct cgctcatcgt cgtggtggaa gatatctccg
    15841 acctgtgggc gctggagcgg gcgggcgtcg tcaactcagt ttaccacgtg cttggcggcc
    15901 acctttctcc cctcgacggg atcgggccgg aacggctcaa catcggacgg ctgatagagc
    15961 gtgcgcaaca cccggaggtg aaggaggttc tgctcgcgct caacgcaacg gtcgaaggcc
    16021 agtcgaccgc gcactacctg accgagcagc tcgagggcac ggcggtgagc gtttcccggt
    16081 tggcccaggg cgtgcctgtg ggcggtgagc tcgattatct cgacgagggg accctggcgg
    16141 cggcgatcaa ggcacgccgg cgtgtttgaa tgtcgtgaga tcgtgaagtg gtgaattcgt
    16201 gaaatcgtga gtagtcagac gacggcacga gatcacgacc acacgaaata gaggcttcga
    16261 ccatgcggca cctgacactg cggaacggcg ctcgcgtccc ggcgctgggg ctcggcacct
    16321 ggggcatggg cgagcggcgt gggagccgtt ccgcggaagt gaaggccctg caactcgggc
    16381 tcgatctcgg gttgacgctc atcgacacgg cagagatgta cggcgagggc ggggccgagg
    16441 aagtggtcgg cgacgcgatt gccgggcggc gcgatgaagt ctacgtcgtc agcaaggtct
    16501 atccacacaa tgcttcccgc tctggcgcca tcgccgcctg cgagcgcagc ctgaagcgcc
    16561 tccgcatcga gacgctggac ctctacttgc tgcactggcg ggggagcatt ccgctggatc
    16621 agacggtcga ggcgttcgag aagctgaagg ccgacggcaa gatccgcgcc tggggcgtca
    16681 gcaacttcga cccatcggac atggcggacc tcgcgaaggc ggctccgcag ggcgcgtgtg
    16741 tcgccaacca ggtgctttac cacctcggct cgcgcggcat cgaatgggat ttgctgcccg
    16801 aatgccggca gcaaggtgtt gcaatcatgg cctattcccc gctcggacaa ggcggcattt
    16861 tgcggaatgg caggttgaag gccgttgccg acaagcacgg cgtaagcccc gcggccgcag
    16921 cgctggcgtg ggtcttgcgg cacgaccacg tcttcacaat cccgaaggcc gcgagcctgg
    16981 agcacgtccg cgcgaacgcc gccgctgccg atctcgtgct cgatgaagag gatcttgcga
    17041 gcctcgacaa agcgttccct ccgccaagtg ggccggaacc gctcgcgatg ctctgacgag
    17101 gaaccaattg gttccaaggt aaaattggcc gcgcagcgct gccagccggc tggagaatgc
    17161 tggcgcgcgg cgcggcctcc cggacaaccc atgttcgggg gccccagcac gggttgtctt
    17221 cccgaatgag atgcggaaag tccgctctct gttcattgtt cctcagagct gggccgtcct
    17281 atcgccggat gcgaggacgg cccagacgac cccgccagga tctggttaat cagccgatag
    17341 ccctcaaaac gagctggcgg agcttcgatc cagttcacct gcccatgcag gtccgtggac
    17401 cggggccatc ggtagtgaag aaatgattgc catcaacatt tccgttgaac gaaatgtcgc
    17461 gactgccgcc attgcggagc ccgccaaggt tcaacggcga cgccacttct aggacaagag
    17521 cgcccgtatc cgcgtctccc aaatggggga tgacacggtc tgtatacaca ttttccttcg
    17581 ccagacggga ttttgccgag gacgattcgg ccaaatgctg aagaagtggg ccgagcttcc
    17641 cgtgcaggga tgtagccgcc ggatcacaat cactcgttcg tctaactggc cgaatcagcg
    17701 cggacgatgc cgctctggct gattgaatgt tgctcagcta acacggcaat atgcggcaat
    17761 tcagccacat ttaggctgaa attgagactt ttcgatcgcg gcttgtacgg atgatattgc
    17821 gtcgaacatc gaaccatcgc acacattgcg tgtgttgtgc gttttaccac aaccaatgcg
    17881 agagattcag atgaaacatt acgagagacc tgttctggtg aagatggccc gtttggcaga
    17941 cattactgcg ggctcagact ttttcagtcc ggccgtcttc acgtcagagc cgccgcctcc
    18001 gccgccaccg cctgacgaga cgccgaagga ccacgacaag gaccacgaca aggaccacca
    18061 taaggatggt ggctggaaag gtggcggcga ctggaagggc aagagctaaa tcatacgccc
    18121 gtagttgcct ccaaccacgt ttgctactca ctccgatgcc tgcgggggtc ggagtttttt
    18181 tttgaatgac ttctacgact aatgaggcgc gtgccgcctt ctgaagccaa cggatccaat
    18241 gttgacgtat gtcattccgg aaaatcgcag aaacattacc gacggacttc cgccacctga
    18301 agcgtttaag ctttggtgac cggtttgagg tccatttttg ttcagaggta accgactgag
    18361 gaggagtaca ccgtgttgaa agttcttata ccagcggcga tcgcttcgct gatcgccatc
    18421 accccggcct cggccatggc tccgacgcag gccggaacgc aattgaagcc gctgaacgtc
    18481 tccgatctcg tcaacgtggc ctcgcgcaag cgttacaagc ggcacagtca tccgaagtgg
    18541 cgctatgttc ccggccgcag atattcgcac gccccgcgtg gctggcgccg gtaccaccgt
    18601 cggccgtact tctggcgtac ccgcggctgc atcatcgtcg gcccggtctg gttctgcccg
    18661 tattgatcga tcagctcttg acgccggccg atacgccgtg cgtcgcgagc atcgatcctc
    18721 gaatgagcgg cgggactctc ctgccgctca ttctttgtcg accacctgaa aacccttcca
    18781 gtacggatcg cggtcgtcga caaaaatggt attgaagccg gtgattcgtg cccagccttg
    18841 gatcgatggg ataatcgcat catagtcgcc gaccttggcc cggccggcga tacggccgat
    18901 gaactgtgag ccgatgatgc tttcgtgcac gaactcctgg ccttcgctca gccgtccttg
    18961 ggcggcccat tgcgccatgc gggccgaggt gcctgtgccg cacggcgagc ggtcgatggc
    19021 cttgtccccg tagaacacag cattgcgcgc ggtgctgtcg ggcttcaccg gcttgcccgt
    19081 ccacatgacg tggctcaagc ctttgatctc gggtttttcc gggtgcacga acgagaacgc
    19141 ctcgccgaag acgcggcgca cgaccgggct gagcgccacc acctcgctcg gctggatgtg
    19201 ctcgagcccg cggaaggtct cctgcgggtc gacgatcgcg taaaagttgc ccccgtaggc
    19261 cacgtcgact ttcagtcgcc cgatgccggg aacgtcggcc tcgaggccgg acgcgtgcag
    19321 atacgacggc acgttcgtaa tgcgcaccca ctccacgaat ggtccgtttt ggccatactc
    19381 ggcgacgacg accccggctg gcgtgtcgag ccgaagttga cccggcgtgc gtggctgcac
    19441 gagcccgcgc tcgatggccg tcgtcaccgt gccgatggtg ccatggccgc acatgggcag
    19501 gcacccggac gtctcgatga aaaggatggc gacgtcgcaa tcctcgcgcg tcggcggcat
    19561 caggatggag cccgacatca tgtcgtggcc gcgcggctcg aacatcaaag cggtgcggat
    19621 ccagtcgaac tcggcgagaa aatgcgctcg cttctcgatc atcgtcttgc cgctgagcgc
    19681 cggggcgcca ccagcgacga cgcgcaccgg atttccgcag gtatgtccgt cgatgcagaa
    19741 gaaggtgtgt gtcgccatcg cagtgaattc tcctgatcag aagcgctgcg cgctgtaagg
    19801 ggagagatcg atcgcaggct gcgcgcccga aatcagtgcg ccgaccagct cggcagtggc
    19861 tgcagcgtgc gtcaatccgt gatgggcgtg gccgaaagca tagacgatcc tgctgctcca
    19921 gcgcgaggtc gagatgaccg gcagcgaatc cggaagggac ggccggcagc ccatgtagtc
    19981 gacccggcct tcttcctgta ggccgggtgc gaatgcacgg gccttggcga gcagcgcctc
    20041 gagccgtttc cagttgggtg tcgcggaggt gctggcaagc tcgacggcgc ctccgatccg
    20101 caacccggtc ttgagcggcg tgaacacgaa ggcgtgctct tcgaacatga tcggacggga
    20161 gagcgtgatg cccggttttg aaatggtggc attgtagccg cgctcggtat ccagcgggac
    20221 tttgtcgccg aggcaggcgg cgagatgctt cgaccaggcg ccggcagcaa tgacgacccg
    20281 gtccgccgtc atcgtgcggc cgccttccag aacgacgacc ggctcgtcgc cgctggcgat
    20341 ggcgatcgcg ctgacgttgc cttcgagaag ctctgcgccc cggtctgcag cggccgacgc
    20401 gagcttcaga gcgagctggt aaggatcgtt cacatgcgcg acgctcggaa agaacgcagc
    20461 gcgctggaac ctctcggaga gggccggctc catagcccgc aagaccgagc cgtcgatcag
    20521 ctcgatcgga atgccgagtt cgacctgctc ccggtagccg gccttgcccg cctcgaacac
    20581 atcgacgttc tcatacacgt agagcatgcc gttgcggttc aattcccgct tcaggttcag
    20641 ccgaccgagc atccgctccc atgccggcag ggccatcatc tgcaggggca cgatgccctg
    20701 cttcgagcgc tgcatgcgcg agggggtgct ggcgagcagg aaccggaaca gccacggcgc
    20761 cagtttggcg gcataagccg gacggacggc cagcggccct agcggatcga tgaaccagcg
    20821 ggccgcctgg agcagcatct gcggtgaggc gaggggctgg atgtcgacat gagcaatgat
    20881 ccccgcattt ccgcgcgagg ccgcggtctg ctctccacgg tcgacgagtg ttactgcaca
    20941 gccctgatcg agcagccgat gcgccgctgc cagcccgatg atgcccgctc cgatgatgat
    21001 gacgtgcatg cgagcggtcc gcctcagttt ccccagtcgt gaggttccgt tcttgcccga
    21061 cgggattgcg gatgtcgagg tctcgcaagc ccccgcgtga aatcagcctg tcccgcgttg
    21121 atgagttgac gagcacgcga tgcggcgtga tatcgatcaa tgagaccggt gagtccggcg
    21181 aggacattcc tttcaagcgc gcaaatctct ggcaggtacg gaaaatggcc aacacgacag
    21241 atgggaagcg gatgaagctg cgctcccagg cctggttcga caacccggac aatatcgaca
    21301 tgacggcgct ctacctggag cgcatgacca attacggcct caccatcgag gagctgcagt
    21361 cgggcaaacc gatcatcggc atcgcccaga gcggctcgga catcactccc tgcaatcggc
    21421 accacctcga gcttgccaag cgcgtgcgtg agggcatccg ggaggcggga gggatcgcgt
    21481 tcgagttccc ggttcatccg atccaggaga ccttgaagcg gcccaccgcc gcgctcgatc
    21541 gcaatctcca gtatctcagt ctcgtggaag tgctctacgg ctatccgatc gacggggtcg
    21601 tcctgacgac gggctgcgac aagaccaccc cggcgcagat catggccgcg gcgaccgtgg
    21661 acatcccggc cattgtcctc tcgggcgggc cgatgttgaa cggttggtac aacggcgagc
    21721 gcaccggctc cggcaccatt gtgtggctgg cgcgcgagat gctggccaag ggcgagatcg
    21781 accacaagaa gttcgtcgag ctggtcgcct cctcggctcc atcggccggg cactgcaaca
    21841 ccatgggcac cgccacgacc atgaactcgc tggccgaggc gctgggaatg agcctgcctg
    21901 gctgcgcagc cattccggcc ccacatcgcg atcgcgggca gatggcctac ctcaccggca
    21961 agcgggccgt cgagatggtg catgaggatc tgaggccgtc caagatcctg acccgcgccg
    22021 ctttcgagaa tgcgatcgtc gtcaactccg ctatcggcgg ctcgaccaac gcaccgatcc
    22081 acctcaacgc gatcgcccgc cacgtcggcg tcgagctcga caacgacgat tgggagcgga
    22141 tcggctacga catcccgctc ctggtgaacc tgcagccggc gggcgagtac ctgggcgagg
    22201 actattaccg cgcaggtggc gtgcccgccg tggtcgccga gctcatcgag aaggggaaaa
    22261 tccgcgagga cgccatcacg gtgaacggca agaccctggt cgagaactgc aaggggcgct
    22321 tctccacgga ccggagggtg atcaagccct atgacgagcc gctgaagccg cacgcagggt
    22381 tcctgaacct caagggcaat ctgttcgaca gcgcgattat gaagacctcg gtgatcgacg
    22441 aggaattccg caagcgttac ctgtcgaatc cgaatgatcc gaatgccttc gagggcaagg
    22501 cggtcgtatt cgacgggccg gaggattacc acgcccgcat cgacgatccg gcgctcgaga
    22561 tcgacgagaa cacgatgctg ttcatgcgtg gcacgggtcc gatcggctat cccggcggcg
    22621 ccgaggtcgt caacatgcgg actccggcat atctgctcaa gaagggcatc cgggcgctgc
    22681 cctgcatcgg tgatggccgc cagtcgggca cctccggcag cccctcgatc ctcaatgcct
    22741 cgcccgaggc ggccaacaac ggcgggctgg cgctgctcag aacgggcgat cgtgtccgca
    22801 tcgatctcaa caagcgtacg gccaacatcc tgatctctga cgaggagctc gagcagcggc
    22861 gcaaggagct gatggcggca ggcggataca agtatcccga gagccagacg ccctggcagg
    22921 agatccagcg cagcctcgtc aacgagatga gcgagggcgc cgtgctgaag aatgcggtga
    22981 agtatcagcg tattgccgag acgaagggca ttccgcgcga caatcactga gctggccgga
    23041 ggatcatctt gggtccgaaa tcgacggccg ccacgatgtc ccggccctgc gctacgagga
    23101 tgcgaggccg tatgatccgc gggcttaggc tcgcggcacg gtaaacaaat aacaaaagaa
    23161 tgcgcccctc tccctccccc tcttcctagc cgggagaggg ttggggtgag cggggcgcca
    23221 gtggcgaaag gtgtaattcc tctttcggcc cttcgggccg acctcttcct gcggaagagg
    23281 tgaatggggc aaagctcaaa ttcgcaaacg ataggctgag aaagtcgcag tcgcgccggc
    23341 tcaagccgat atgaaagcga gcaggtcgct ggtgagctgc tcccggctcg tatcggctag
    23401 cccgtgtgct cctcccgggt agaccttgag ctcggcatgc ttcacgatct tggcggaagc
    23461 acgccccgcg gcatcgatcg gcacgatctg atcatcgtct ccgtgaacga tcagggtggg
    23521 gacgtcgaat ctcttcaggt cctcagtgaa atcggtctcg gagaaggccg cgatgcagtc
    23581 gtatgcgttc ttgtgcccag ccatcattcc ttgcagccag aagctgtcga tcgaaccctg
    23641 tgagaccttc gctcccggcc tgttgaagcc gaagaacggc cccgaagcga gatcccggta
    23701 gaattgggac cgattggcga ggctggccgc acgcagctcg tcgaagacac tgatcggcag
    23761 tccgccggga ttgtcggggg tctggagcat caagggaggc acggcgctga cgagagcgac
    23821 cttggccacg cgctcggttc catgccgccc gatgtagcgg gcgacctcgc cgccgcctgt
    23881 cgaatgcccg accagaatga cgtcgcgcag gtcgagcgcc tcgatcagcg ttgcgagatc
    23941 gtcggcgtaa gtatccatgt cgttgccggt ccagggctgg cttgaccgcc cgtggccgcg
    24001 acgatcatgg ccaatcgcgc ggaagccgtt ggacgccacg gccatcatct gcgcttccca
    24061 ggcgtccgag cagagcggcc agccatggct gaaaaccacc ggctgccccg agccccagtc
    24121 cttgtagtag atctgggtgc ggtcctgcat cgagatcgtc ggcatcatcc ggacctcact
    24181 ctgacggaag gttgttccgg ataacctgag ggacgggccc gggttgccag ggtctttcct
    24241 cgagcgccgc ctttttcgtg atgatgctta tcgcccatca ctccgcggct tgctcggcat
    24301 gtcgcggtgc gttggcttcc tctttgggtg caaggctggc ggcgagggct tcctccacgc
    24361 gctcgatcca gatgaaatcg agctcctttc gtgcttcttc aggaatatcc tcgtagtcct
    24421 tccggttgcg tatcggcagt atgacccgcg tgatgccggc ccgagccgcg gcgacgacct
    24481 tttccttgat ccctcccacc ggcagcacga ggccgcgcag actgatttcg ccggtcatgg
    24541 cggtgtcgct gcgcacgcag cgccgggtca gcagcgagac gagcgccacg tagatcgcca
    24601 cgccggcact cggcccgtcc ttcggtacgg cgcccgcggg cacgtgaatg tgaatgtcgt
    24661 tcttttcgaa tcgcgctgga tcgatgccca gttggtccgc gcggttcttg acgatgctga
    24721 gggcagcctg cgcgctttcc cgcatcacct ccccaaggtg gcccgtgagg atcagcttac
    24781 ccgagcccgg gatgcgggtg gcctcgatga aaaggatgtc gcctcccacc ggcgtccagg
    24841 cgaggccggt ggcgaccccc ggcacgctgg tgcgcatggc gatctcgttc tcgaactgcg
    24901 gggcaccgag gatcgcacgg aggtcgccgg catcgatgcg gatctgctca gtctgacctt
    24961 cggcgatgcg cacggcggca tgcctgagcg ccctcccgat ctcccgctcg agattacgca
    25021 cgcccgcttc gcgggtgtag tgctcgatga tctcgttgat ggccgcgtcc gtgatctcga
    25081 cctgctccgg cttgatgccg ttcgcctcca tctggcggcg gaccagatat cgccgggcga
    25141 tctgcagctt ctcctccgcc gtgtagcccg ggaggctgat gacctccatg cggtcgcgca
    25201 gtgggcctgg gatggtgtcg aggatattcg cggtcgtgat gaagacgacc cggctcaaat
    25261 cgaacggcac cgcaaggtag ttgtcgcgga acgtgctgtt ctgctccgga tcgagcacct
    25321 ccagcaacgc cgctgccggg tcgccgtgaa tgccggcgcc gagcttgtcg atctcgtcca
    25381 gcatgagcac gcaattgcgg gagccggcct tgcggatggc ctggatgatg ttgccgggca
    25441 atgcgccgac gtaggtgcgg cggtgtccgc gaatttcggc ctcgtcgtgc accccgccga
    25501 ggctgacgcg ctggaacttg cgccccatgg cccgcgcgat cgactggccg agcgatgtct
    25561 tgccgacacc cggcgggccg acgaagcaga ggatgggcgc cttgccctgc ggcgccagct
    25621 tgcgcacggc caggtactcg atgatgcgcc gcttcacctt ttcgaggccg tagtggtcct
    25681 cgtccaggat cttgcgggca tgctcgatgt cgatcggcgg ctcctcgggc agcttccagg
    25741 gcaactcgac gagccagtcg agataggtcc gcaccatccc gtactcggcc gccgcttccg
    25801 gcatgcgcga gagccggcgc agctccttgc gcgcgtgctc ctcgacgtca gggggcatct
    25861 cggccttggt gatggcctgc tcgagctccg ccatctcctg ggctttcgaa tcctcgcctt
    25921 caccgagctg gcgctggatg gcggccatct gctcgcgcag cagcacctcg cgctgacgct
    25981 gatcgagtgc ggccttggtc tggcggctga tctcctgtga gaggcgcagc acctcgatgc
    26041 ggtgcgcgag aagtttcgag accttctcca tgcggacggc gaggttgacg gtctccagga
    26101 tctcctgctt ctccgaggac gggatgtcca tgtaggcaac cgccaaatcg gccagagtgc
    26161 ccggcgaggt gacggactgg atcgtggcga tcatctcctt cggcgcctgc ggcagcagct
    26221 ccagcgcctc gatcgcctgc cgctgcaggt gcaggaagcg tgcctcgatc tcgggcgtcc
    26281 gcgtctcggc atcggggatg cggagcacac gggccacgaa gaagggccag ccgctcagga
    26341 actcggtcac gtggaaccgc tcctggccct ggcagacgat gtgatgggtt ccgtcggtgc
    26401 ccgttacgaa gcggacgatg tccgccaccg tccccatccg atagagatcg acaggggcag
    26461 gatccgccac ctccgggtca cgctgggtca gcacgccgat ctgccgctgc tcgcggacgg
    26521 cctgctgggc ggcgaggacg gatttctccc gcccgatggt gatcgggaag acgactttcg
    26581 gaaacagcac caggtcccga acgggcaaaa tgatgagcgc atcggcgggc gggctgggga
    26641 gctccggtcc gcttttcgag cgccgcgggg cctcctgttc ggccatcgat cttctccacg
    26701 tctagtattc tgcagtcttt tccaggcgga tgagcagaca gccctggctt gccgaccagc
    26761 ggatcgcccg atagctgccc tccggcaggc ggatgcggcg ttcgaagcgc ccctggggca
    26821 gctcgagacg gtggatcgca gccgtgcgga aggcggcagg cagtacgcgc gcgccggcga
    26881 tcaccaggtc gcagttctcg atcaccgcct gcacctcgtc tggatcgacc cccggcagcg
    26941 cgatgaagac caggacctcg tcctcggttt caagaacgtc gaccgggggc cgccaggagg
    27001 gctgtcgccc cacccccgcc tggggatcga ataatttatg gtgcagccgc tcggcgcgcg
    27061 ccagggcctc gcaggcttcc gaccacatcc aggaagggaa gttcttgtcc attggcgtta
    27121 tccacggtga tcgactaggt gacgccttcg gtccgtcagc tcacccgctg cccgggtcga
    27181 cgtccttgtc cttgctgatg tcgggcaggg cgcctgcgcc gggcgtggcc tcaggatcgg
    27241 taagctccgg tcgcgcgtgg ggcccggccg gatgcatgaa cgtcttggat cggcggctct
    27301 ttcggagggc ttccttctcc tccgattccc gtgcgggctt cttgcgcggc atgtgattgc
    27361 cctcccgagg tggccccgtc atttgcagca gacgtagggg gaacgccccg gcgagcgtat
    27421 ggttcaggat gccgcgccgc agcagcaatc gctcccgcgg cggtcccggc tgcagggtgc
    27481 ggccattctc ccgctggcat ggaatggctc ggtttcctgg cagctcttca ggaacctccg
    27541 gagggcttgg gcgttggggt tcggaaagca tcgtgctgca ctgcaaacgc aggaagggcc
    27601 ggtctggttg cgcaccctcg cggcatgcca tccaatgcta acggaccgcg gcacgcttgc
    27661 tctgacgtag gtcaatcttg gaggtcggca tggatagccg aatttggtgg ctgctcggtg
    27721 cggtggtcat aatcgtgata ttggtcgcac tgctcttccc gaccggtgag ccgacgacga
    27781 ccgcgacgac cccgcccgcg acaaccgctc cggaatcgac gagtccgcca gcgaagacga
    27841 caccctcgac tccggaaaaa acctctccgc cagccgggca gcagcagtga gctggtgaag
    27901 agtgggtcga tcgaatttgc gagccgcaaa cctcaaggtt tgcggctcgt cagtgccacg
    27961 cctgtcagaa tcagcatgaa acccacgatg tgaaaggccg cgagtggctc tccgagcaat
    28021 ccgatggcca tcaatgctcc gaacagcggg atgagatgca tgaatatccc ggcgcgcacg
    28081 cccccgatca gctcgacccc gcgcgtgaag cacagatacg agagtatgct cggaaaaatc
    28141 gagacgtagg cgatcgccag caccgtaaag agcgtcggct gaacgaactg gccgaagacg
    28201 aacatctcga tcagcaccag cggcaggttg aacaggcccg atgccaggaa catggtgaag
    28261 agaaacgtga tccaatgaat ttcgggcctg agccgcaggt aggccgtgta gaccgcagtg
    28321 atggccatcg cgaacaggat catcagatcg ccgcggttga ggctcaaggt cgcgagcacc
    28381 tctggctttc cccgcgcgac cacgaccagc gcgcccgtga gcgagatgac cgtgccgacc
    28441 atctgggtaa cggtcagccg atccttgaac agcagcacgc aggcgatggc gatcatgatc
    28501 ggccccatcg aattcagcac gaggctgttg agggcagtcg tgtagttaag accgatgtac
    28561 tggagcgtgt tgaacgcccc tccgcctatg acccccagga agccaagaaa gagccatttc
    28621 tggcgcacga ccggccaatc gcgccgcagg tgaggcaacg cgaacggaag caggatgagc
    28681 gtggcgagag accagcgcag gaacgacagc gcaacgggcg gcacatggcc cgccaccgct
    28741 cggccgagca cctgattgcc ggcccagaac agcgccatca ggctcagaag caggtaggca
    28801 ttgtcgaagg ccttctcggc caggcgaggc agcatgcaca ggtctcggtg aaaaagcgct
    28861 gcccaccgta cgcgatgggc agcaggcaat caggagttat gggtggcgaa ctgtcttgct
    28921 acctgcccca ccggagtgca agaggcgaga cctgctcggc gagctccagc agctgggccc
    28981 gggttgccgg atgcagcggc tcgagcgggt ggcgcactgc atcgctcttg atgacaccgc
    29041 ccgcctgcat gacggtcttc gtggcgcgca ggccgcattg gcggttctcg aaattgatca
    29101 gcggaagaat gcgcgcgtaa gcgtccatcg cctcggcacg ccggcccgcg cgatgatgat
    29161 cgagcaccgg cttgatgaga tccggcagca gggcgctggg catggtgccc gtggcgccgg
    29221 catcgaggtc cgccatcaga gtgattgcct cttcaccgtc gaacggcccg gcgatggcat
    29281 cgccgcccgc ctctatcagc gcccgcagct tcgccgccgt gcccggcacc tcgatcttga
    29341 agtagcgcac caatggcacc tcgcgcgcca gccgcgtgag aaatggcacc gacaactcga
    29401 cgccgctcaa cggcgcgtcc tgaaccatga tgggaatgcc ggcggcatcg gccacccgct
    29461 ggaagtgttc gaggataccc ttctcgccag ctttcaacgt ggcgccgtgg tagggaggca
    29521 tcatcatgac catgctggcg cccgcctttg ccgcggcccg agctctctgg gcagcgatat
    29581 cggtgctgaa atggctgcag gtcacgatca ccggcacgcg tccggccaca tgggcaaggc
    29641 agacctccag tagcgtgttg cgctcctcgt cggaaagcag gaattgctcc gagtagttcg
    29701 ccagaaggca gatgccgtcg acgccctggt cgatcatgca gtcgagcacg cggcgctgcc
    29761 catcgaggtc gagcgagccc tgatcggtga aaggcgtcgg cgcaatcggg aatacgcctc
    29821 tgaatggctc acggctcgtc tgctgcatcg cttcgctcct cttctctcgg gcacgcatga
    29881 ttcctgatga gccatagcac gagtttcagt ctgggcagcg cggcttatcg gaggcggcga
    29941 aattcggacc ttgagtcggg gcaggcaccg gcagcctcgc gggcgcggcg gagcgctgct
    30001 gcccagttgc gcatcccgcc ggctgcgcta acttctcccg cccatgcgaa aggaactcga
    30061 aatggcaggc gcaaacaaat cggagggagg tcagcaggtc cagcgcgaca agccctggct
    30121 gttccggacc tatgccggcc actcgacggc tgccgagtcg aacgcactct accggcgcaa
    30181 cctggcaaag ggacagacgg gcttgtcgat cgccttcgat ctgccgacgc agacgggcta
    30241 cgacagcgat cacgagctgg ccgccggcga ggtcggcaag gtcggcgttc cgatctcgca
    30301 catcggcgac atgcgcacgc tcctcgacgg cattccgctc gaccgcatga atacgtcgat
    30361 gacgatcaat gccaccgcgg cgtggctgtt cgcgctctac atcgcggtgg ccgacgagca
    30421 gggcgcgccg cgggacaagc tcaccggcac tatccagaac gacatcatca aggagtattt
    30481 gtcgcgcggc acctacgtct tcccgccgga gccgtcgctg cggctgacca gggacaccat
    30541 cgtgttctcc tgccgcgagg tgccgaagtg gaatccgatc aacgtctgct cctatcacct
    30601 gcaggaggca ggcgcgacgc ccgtgcaaga gctcgccttc gcgctggcga ccgcgatcgc
    30661 catcctcgat agcgtcagag actctggtgc cgtcgatcgg gaagctttcg aagccgtggt
    30721 cggccgcgtg tcgttcttcg tgaacgcggg gctgcggttc atcaccgaga tgtgcaagat
    30781 gcgcgccttc gtcgatctct gggacgagat cacgcgcgag cgctacggcg tgacgaaccc
    30841 gaagcaccgg cgcttccgct acggcgtgca ggtgaactcg ctgggcctca ccgagcagca
    30901 gccggaaaac aacgtctacc gcattctgct cgagatgctg gccgtcacgc tgtcgaagaa
    30961 cgctcgcgcg cgtgccgtac agctgccggc ctggaacgag gcgctgggtc tgccgcggcc
    31021 ttgggaccag cagtggtcgc tgagaatgca acaggtgctg gcctacgaga cggacctgct
    31081 tgagtatggc gacatcttcg acggctcggt cgagatcgag cgcaaggtcg aggagctgaa
    31141 gcgtcaggcg cgggaggagc tcgccacgat cgagcggctg ggaggggccg ttgcggccat
    31201 cgactacatg aaacgccgcc tcgtcgaaag caacgcggcg cggcttgcca ggatcgagtc
    31261 gggcgagcag gtcgtggtcg gcgtcaatcg cttcaccgag accgagccat caccgctgtc
    31321 ggccggcgag ggggccgtgc agacagtggg cccggaggtg gaggtcgagc agatcgcccg
    31381 tctgaaggct tggtgcgagg cccgcgacga ggccgccgtc gccagggcgc ttgccgatct
    31441 cgcccaggct gccaaggaag accgcaatat catggagcct tcgatcgcct gcgccaaggc
    31501 cggcgtgacg accggcgaat ggggcatgac gctgcgccgt attttcggcg agtaccgtgc
    31561 gccgaccggc gtcggtcagg ccgccggcga gggagtggtt gggctggagg cgcttcgcga
    31621 gcaggtgagg gcagcagccg cgcaactcgg gcgcccgatc aagctcctgg tcggcaagcc
    31681 ggggctcgat ggccactcca acggggctga gcagatcgcc gtccgggcgc gcgactgcgg
    31741 aatggaggtg gtgtacgaag gcatccgcct gacgccgaag cagatcgtgc gggcggcctt
    31801 ggaggaaagc gtgcacgtcg ttggcctgtc ggttctttcc ggctcccacg tgccactggt
    31861 ccgtgcggtt ctcgacggca tgcgtgccga gaacatggac gatatccccg tcgtcgtcgg
    31921 tggcataatt ccgccggagg atgcagcttc actcaaggcc atgggcgtgg cccgggtgta
    31981 cacgccgaag gatttccagc tgaacgacat catgtcggac attgtgcagc tcgtcgtccg
    32041 ccagccagcc gcagcgtgag ggggacatgg ccaagatcag cgaagagacc cggcgcaagc
    32101 tcgatcgaat tctgctcatg gagcggttga agtggctcgc tgcagccgtg atcgcgattg
    32161 ctgcgatcgt cggctttttc atcctgatcg acctcgatgc cgaggtcgtg gattatcagg
    32221 tcgccggcaa ggtggagcac gtcggcatcg cgaacctcaa gaccgtcagg caggccgttg
    32281 aagtcgacgt ggccctggac gatggtcgtc acgcccgcgt gatcgcggcg aaggagcatg
    32341 agccgcatgt cggcgatcgg gtcaggatcg tggagcacaa gcacgccacg ggccgggtaa
    32401 ccttcacctg gcgataggtc gcggccccgt gggcgtgtga agtcgcgatc acttctcctc
    32461 gccgctgatt tccactttct cgcccagtgt ctcacggatg tagagcctgc ggaccgcgac
    32521 gtctgccacg acggccagcg gataggcgaa cagcaccccc agcgggccaa agagcacccc
    32581 gagcgcgatc acggcgaaaa ggcccaaggc aggtgggatc ttgacagcgt tgccgacgac
    32641 gatcggcacg atgatgttgg attcgatttg ctgcacgatg acatagacgg ccagggtcca
    32701 tagcaccatg tcgaagccct gcccggatgc gacgaggagg cctggaatcg cggccgtgat
    32761 cggaccgacg atgggcacga agtcggccag accggcgatg aagcccagcg ccagcgccga
    32821 gttcaggccg atcagccaca gcccgagcgc cgtcatgacg ccgaccatga gcatcgcaat
    32881 gagctggcca ccgatccaaa ggctgagcgc acgcccggca tcgtcgaccg tttcctcgat
    32941 gagcggctga accctcggtg ggaacaggag aatgaagccg tttctgtacg ggatcggatc
    33001 gagtgccagg tagatcccgg tgaagatgac gaacaccaag gccgtggcga tgctcacgat
    33061 cgccgtcgtc cattcgtatg cggtcgtaag aacattgccg acggcggtcc ccgtcagcat
    33121 gtcctggaca cgggtgacgc cgaactcggc caggatccgg ttggcagctt cagggaggac
    33181 cgtcaggagc tgattgaact ggccggcgat ctgcgcgccg aacagggcga ggccgctcac
    33241 cagaacgact aggatgacag cgccggacag cgagagtgcc acaccctccg aaacgcggag
    33301 tttggttttc aagggggtgc tgatcgagcg gacgaggacg gccagcagaa cgccggcgaa
    33361 cgtgagcagc agcacatcgg acaggaacca gatcgctgcg gccagaaccg cgaatgctgc
    33421 aaggatcaag attcggcgaa ctaatctcac ctcggagtcg ggcaaatcgt ccgggcgggg
    33481 cggatcggcc aagatgattc tctcgcgtcg gcaacggatt ggcagtctaa cagcacaaac
    33541 ggatgcgcgg attgcctggt tccgacctag gtcgccggct tcgctcacaa ctccgagcct
    33601 cccagccttg gggaattatc gcgtcgtcca gcaagcagct ctcaggcgcc gttgacggcc
    33661 cgtccgcgac ggctatgatg ccgccccatt cagggcctcg gccccagtgg cggaattggt
    33721 agacgcgcca gactcaaaat ctggttcccg caagggagtg cccgttcgag tcgggcctgg
    33781 ggcaccatta tcaagacgca cgtaccgcga tccgcggcga ccagcgccgt cggaggcacc
    33841 tcgccaattg gccggcatcc ggctgtggcg tcagcggatg gctcaggagg gagcgcgagc
    33901 ctcacccttg atgagggcgg cgcccttctc accaatcatc agtgatgccg cgttggtatt
    33961 ggccgacgtg atgagcggca tgaccgacgc atcgaccac
//
LOCUS       DASVDU010000119        17723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_210061, whole genome shotgun sequence.
ACCESSION   DASVDU010000119 DASVDU010000000
VERSION     DASVDU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210061"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2242
                     /locus_tag="VF226_07630"
     CDS             <1..2242
                     /locus_tag="VF226_07630"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7073895.1"
                     /translation="ATTFLSALLLFSIQPLFAKMVLPVLGGAPSVWAVALCFFQGALL
                     VGYCYAHLLNRLVPARAAGFVHLAVFGLALLSLPIGLPAGWAEPPPGDPYFWQLGLFS
                     AAIGMPFLAVAANAPLLQAWFAASGHPDARDPYFLYGASNLGSLVALLGYPFLLEPTF
                     GTRALSDFWAAGFVLLMVAIAACFLVLRGRAAASVPTATMAAGTADQAPSWPRRLGWV
                     GLALVPSALLTAFTTHISTDVASAPLLWVLPLALYLLTFVIVFRERALIPMRLLLIVH
                     LAAVVAALLQLSQTKHDGWFISSATGVVVFFTSALVAHRTLYDLRPAPRYLTEFYLWM
                     SLGGVLGGLFAALVAPHLFSEVFEYPLLLALTMACRPGALSISLRDREELLRIWLVVA
                     GGVLAIYWAPWLVEQLSDISPGGIVGTAIDKLLEWGTAAAIVLAFAILLVVSLRHPPR
                     QVAIAMLMFLTVVTLPSGVKRGDAQRSYFGVYRVSLSSDGNFHVLTHGTTLHGAQRIR
                     DSEGAYKDDLTPTTYYHPKSPMARTIAEVRGRLAWQGRRGRYGVIGLGTGSLACYARQ
                     GERWRFFEIDPLVVNIASTPEQFSFLHYCQPEPDIVIGDARLTIAKEPDASFDLIIVD
                     AFTSDAVPVHLMTAEALRLYASKLTADGIVLLHISNRYLDLDSVLAATSPLAPELHGL
                     ILSDDDADGSYASSTSTVAVFSKDETVINGYRDWPEAYELEANGLRGWTDDFSDILGP
                     LLAKLR"
     gene            complement(2267..3211)
                     /locus_tag="VF226_07635"
     CDS             complement(2267..3211)
                     /locus_tag="VF226_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEX7073896.1"
                     /translation="MKINWRVLRGFLGAVLIIVAAALLIGVEIAEAQRRPGPEPPVRS
                     PAVAPKQPVRSAPVAQAETRPPQPVPAEAGPRETVQADVSSRSVSVTSSFTGTEIVVF
                     GAVDHSRQTTAESGLYDVVIVLEGTPTRLVARRKSNVAGIWINTHSITFESVPSYYAI
                     LSTRPLDDITNPLVLRENDIGFEYVRMTPVQGWETGVTTAELQEFAEAVVRLKQKDGL
                     YVQEEYGVVFIGRSLFRASVNLPANVPVGPLDVRVYLFRDGQIISTYTARVRLEREGL
                     ELLLHNFAFNQPLLYGIFTVTIAVGAGLVASAAFKRRQ"
     gene            complement(3211..4137)
                     /locus_tag="VF226_07640"
     CDS             complement(3211..4137)
                     /locus_tag="VF226_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7073897.1"
                     /translation="MNLYLPIAEMSVNVFAFLGMGGAVGFLSGMFGVGGGFLMTPLLI
                     FSGIPAAIAVGTEAAQILASSVSGAIAQWRRNNVDVKLGTVLLSGGIVGAAAGVEAVK
                     LLRQIGQFDLFVSIAYVTFLGIIGVLMLIESAKAMRQARHGKVMSMRRPGQHNWVHKM
                     PLRMRFHRSKLYISIIPPFAIGMLVGFLGAIMGVGGGFIMVPAMIYLLRVPTNVVVGT
                     SLFQIVFVTGATTILHAAQNKSVDVTLALLLMIGGVVGAQLGAAAGQKLKGEQLRFLL
                     AGLVLLVCVRIGWGLVTAPSDEYSISDAIGSH"
     gene            complement(4279..4662)
                     /locus_tag="VF226_07645"
     CDS             complement(4279..4662)
                     /locus_tag="VF226_07645"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073898.1"
                     /translation="MVDRLWPRGLNREKAAIDEWAREIAPEPRAALGVCCQSDKVKGL
                     RALPAEGSRARRIASRFPRVCAKSRAEAGHAAFCEKGEVRNSAVVLKAVLDEVCWSPS
                     LSDFSSVRDLCGPEPGSLGCPAKYG"
     gene            complement(4767..7655)
                     /locus_tag="VF226_07650"
     CDS             complement(4767..7655)
                     /locus_tag="VF226_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073899.1"
                     /translation="MSFNNNQGSTGGFRHEVSPPSSYSVCGAGELKLLLQRIAEYIAN
                     ADERQQAALGEMQSRLAHLADEAQSVRTSITPELAAAFARIEESISTLTKRVAADDHG
                     GTAQPSRASELDRHTYWPGSSHDDPAFGPKPGESNARYSEPIWSRSRTDPPPPLRSAA
                     GPSHFESLEAARPRPSYASAMADPYPDIPDAGDHGVWDTASAEALARSYEPEHFGDKP
                     RGQSFQNSHTQQHAGQPYHALAPERSDGQGNGTTGDHFEQRFAELADRVEEALVELRT
                     NSSVTALRARLDSFDQKLGSAISELATRADVEKLRAVEAHISELARHVDRAQHRLARL
                     DEIEHNLTRLIDHLSDERLAQLFDQRILSEPRLAALAEAVAERLSARETARGDFGSGL
                     DRLGELHDIIEQFVNAQRREGEQTASALEAIQEAMLNVLDRMEALEGAHTPAPPARRA
                     IEADWRHESDGLAAADSAYGLQQNDQPTVSSVFPREWPRESKPASEQPGIEAELEQLR
                     STVRIERADQASAEAASREEATAVVKAENGTESSPLRASREDFLAAARRAARKASAQT
                     MEETADVSQAKVRLQQAGNRRAAGARPVTGLLVATLAVALAVGIGVTTYSIFKGDLAK
                     PWGLVRGALVKGGTAATAPAGAGQGKAAGGLSDGGSSSAPAGTTTPEVIIDPPGARSG
                     PGEDDSLTGGPRSVTTLPGGIIIDNGSLPPVISAAPARAAFSPDTRSVTPQPAQAVPP
                     VPARRMMPPAAVGPLSLRLAAANGDPSAEFEVAVRLDEGRGVERNLKEAVIWYQRSAS
                     RGFAPAQYRLGTLYERGLGVAADRGRAMAWYRSAADKGNLKAMHNFAVLSASGDPADY
                     KTAAHWFSEAAERGLVDSQYNLAVLYETGRGVDRDLKQAYKWFALAARSGDKDAMRRR
                     DRVKWLLNANELKAAEGLVQSWHPQPAEPLVNNVRAAGEVWKQREVQG"
     gene            8049..8513
                     /locus_tag="VF226_07655"
     CDS             8049..8513
                     /locus_tag="VF226_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073900.1"
                     /translation="MRALVIAIPAIVAIGLSAANVASQERQGRYVMNPVDNGFVRLDT
                     ETGAMSVCTQRNGRWVCELMEDEAKALQEEIERLKAEVKRLKDQAALAEPSPDRAYPS
                     ERPERSFELPTEEEVDKALDYFENIFRKFRERIERFEKEQRGAEGRKDGTQL"
     gene            8526..8972
                     /locus_tag="VF226_07660"
     CDS             8526..8972
                     /locus_tag="VF226_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7073901.1"
                     /translation="MAEVHVLKQASTTLSISTRGPGFIDLTGRIEDWVDRIGAREGLL
                     TAFVRHTSASLTIQENADPDVQRDLLDALDRLAPRSHAYRHGIEGPDDMPAHIRSMLT
                     TTSIGIPIVDGRPVFGTWQALYLIEHRDAPHRREVVLQFIGEVRSA"
     gene            9072..9386
                     /locus_tag="VF226_07665"
     CDS             9072..9386
                     /locus_tag="VF226_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073902.1"
                     /translation="MLILRMLSGIFLLAAALALIAEVTNAQLGIPGAPLTPVYKQLAD
                     AAPAALKAMEQAVSKLHPLLWDPVLLSILRLPAWISLGSIGAVLGILGRRRYQRIRVF
                     TN"
     gene            complement(9399..10484)
                     /locus_tag="VF226_07670"
     CDS             complement(9399..10484)
                     /locus_tag="VF226_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073903.1"
                     /translation="MVEGDDAGKGPPASVQSSTTEADAQPKQPPADVTQAETSNATAT
                     TADTPQRHSSAQSENPASDKALSSSLAATASRELEKPAQSKTPQPTTGTSSGRIVYQA
                     STVRKTILSFIFLILLPFFVSLPAMMIQRAMHQLWPNMVGFTIFAVAFALIMLLVLFE
                     LIHSIRARVAIGESAVRLTLPAARGMAIPKLFYKTREVPYSEIQAVETRREVYGGLIA
                     PVMLRGARIVTKGGDKIPLGYVNEANVDPVMPVPQIAAEIARRAGLEVIDQGTVRRQV
                     HKKLRGIRATDDGANPVSEAEIAALNRRHNTAMLVLCGCLFLLVSGGLMLDISKSDLD
                     LGERAHQLVQSLMKSSPEVQQAGAPRR"
     gene            10626..11291
                     /gene="msrA"
                     /locus_tag="VF226_07675"
     CDS             10626..11291
                     /gene="msrA"
                     /locus_tag="VF226_07675"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7073904.1"
                     /translation="MLFPRKKTQMPTPETALKGRPTPIPTAETHFVNGRPLKGPYPDG
                     YEKAIFGLGCFWGAERKFWQLGDGIWITASGYAGGYTPNPTYEEVCSGGTGHAEVVLV
                     VYDPKKISYEELLKTFWESHDPTQGMRQGNDVGSQYRSAIFTTSPEQFTAAEASKKAY
                     QRVLSANGYGPITTEIRAAPEFYFAEDYHQQYLAKVPHGYCGLGGTGLSCPIGTGVEV
                     ASR"
     gene            complement(11325..12209)
                     /gene="mepA"
                     /locus_tag="VF226_07680"
     CDS             complement(11325..12209)
                     /gene="mepA"
                     /locus_tag="VF226_07680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX7073905.1"
                     /translation="MPAKQLFGKVSGPAELKPEAIGFYARGCLAGGKELAIDGPAWQA
                     MRLSRNRNWGHPRLVALVERLATEAQKYDGWPGLLVGDISQPRGGPMLTGHASHQIGL
                     DADIWFTPMPNRRLTEKEREEMSAVSMLGPDKLSVNPNIWTDSHVRIIRRAASYPEVD
                     RIFVHPAIKKALCEGADKIGSDRSWLRKVRPYWGHHYHFHVRMGCPAGSATCKDQAAP
                     PPGDGCGKELSDWLALIAAPPKPKKPSKPKPPLTLAQLPHQCRAVLSADAPAAATASA
                     PLKVPVPVRATRTKAQQQ"
     gene            12410..13039
                     /locus_tag="VF226_07685"
                     /pseudo
     CDS             12410..13039
                     /locus_tag="VF226_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
     gene            complement(13051..14280)
                     /locus_tag="VF226_07690"
     CDS             complement(13051..14280)
                     /locus_tag="VF226_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7073906.1"
                     /translation="MTHATTRDRVHFVFLNVGHFVDHLLTLVFATVAALALTREWGLS
                     YSELIPYATPGFIAFGVFSLPAGWLADKWSREGMMAVFFVGIGLAAIATGFARTPIEV
                     GIGLFVVGVFAAIYHPVGLALLVERASRTGTGMAIGINGVWGNLGVASAALITGFFID
                     NGGWRLAFFVPGLVSILIGLGYIALFHAEMRPAAGNGRGKSKGAAAPLPPDLKALLLR
                     LSVIIFFTTAVSGIVFQSTTFALPKVFDERLTGIAGSATIIGWLAFLVFAVASIAQII
                     VGRLLDRLGPRTVFIGAATIQLVFFSLMPGLADWAALVVALAFMLGAFGQIPINDYMI
                     GKLARSDMRASIYGVRYIVSFAVLAAALPLIAWIHQGWGFDRLFQVLAGAAGAILIAV
                     WLLPRRLPDPEPVPAAA"
     gene            complement(14318..15031)
                     /locus_tag="VF226_07695"
     CDS             complement(14318..15031)
                     /locus_tag="VF226_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012759399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX7073907.1"
                     /translation="MTWSIIARDEATGRVGIIAASRFFAIGAVVPYIKTGVGAIATQA
                     FVNPFYGPQGLALLQAGATAEDTVKFLTGGDDGRDHRQVHVMDREGRFAAFTGTACID
                     WCGHRVGPHLSVAGNMLAGPEVVDATLRTYEANLQTPFARRLIKAMKAGEAAGGDSRG
                     KQSACLLIHDQEEYPLLDIRADDHPDPLSELERLEAVARERWLHFRRLMPSKAHPSGA
                     ADRVDLEDRIAASIAEGYE"
     gene            complement(15322..15933)
                     /locus_tag="VF226_07700"
     CDS             complement(15322..15933)
                     /locus_tag="VF226_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073908.1"
                     /translation="MAPDFYRERILPEAYGVLELSHFFLPLAGFFLCLGLFSSPLVKS
                     WPLLRAAIVAFALSCFYIAGEEHSWGQWFFHWDTPEFWGEFNRQNETNLHNTSPLFNQ
                     LPQKVLQIAIVVGGLVLPLSRRAREWVAGIPIIVVLTPPRAIVPVALTAVGFNMLVSL
                     QKNELLPELLPRPSEAAETFYYMFILFYLIMLRRRLMQSRGVC"
     gene            complement(16200..16616)
                     /locus_tag="VF226_07705"
     CDS             complement(16200..16616)
                     /locus_tag="VF226_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7073909.1"
                     /translation="MQLSKRILIVEDEPLIAEMLREWVAELGYETVGPAASNEAALAL
                     IAGGPPLRCAILDITVRDGPSYPIARALEQRGIQYAFATGHGTETIDPQFKNAPTLLK
                     PFDFDVLAEVVAHLVRAPAKTSDWASDVVPDSHGRG"
     gene            16859..>17723
                     /locus_tag="VF226_07710"
     CDS             16859..>17723
                     /locus_tag="VF226_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEX7073910.1"
                     /translation="MPVSWLQSVKIRTGALSGIGLDEADRLPRWTIAMFAASFVTLLV
                     AGISAGLIATRSQRVEKVAERTLQVHRLTEHVITMLQLAETSQRGYLLTGDEEFLKPY
                     RTAASELTPEWEKLKQLSQTNGSLDVQLAELSALIDRRLSGLQEAITLLQRGDTERAR
                     DPKRLNSGQAQLLEIRERLTRLSDAEEAQMIRFQAEAAELRETLMPLVAISLAATVVL
                     ALLVGRAITHYISRLVARTAELEREARLRRETEDTLRQSQKMEAIGQLTGGVAHDFNN
                     LLTVILGNLDTV"
BASE COUNT         3223 a         5764 c         5570 g         3166 t
ORIGIN      
        1 ggccacgacc tttttgtcgg cgcttctgct gttctcgatc cagccgctct tcgcgaaaat
       61 ggtgcttccg gttctcggcg gcgcgccgtc ggtttgggcg gtcgcgctgt gctttttcca
      121 aggtgcgctg ctcgtcggct actgctacgc ccacctgctc aatcggctcg tgcccgcccg
      181 tgctgcgggc ttcgtacact tagcggtttt cgggctggcc ctgttgtcgc tgccgatcgg
      241 cctgccggcc ggctgggcgg agccaccccc gggtgatccc tatttttggc agctcggcct
      301 gttttcggcg gcgatcggaa tgccgttcct cgccgtcgcc gccaatgccc cgctgctgca
      361 ggcgtggttc gcggcgagtg gccatccgga tgcacgcgat ccttattttc tctacggggc
      421 aagcaatctc ggcagcctgg tcgctctcct cggttatcct tttctgctgg agcccacctt
      481 cggcacgcgg gcgctgtccg atttctgggc cgccggcttc gtcctgctca tggtggcgat
      541 cgcggcctgc tttctcgtgc tgcgcgggcg cgctgccgcc tcggtcccga ccgcgaccat
      601 ggccgcagga acggccgacc aggcaccgag ttggccgcgc cgtctgggat gggtcggcct
      661 tgcgctggtt ccctcggctc tgctgacggc cttcacaacg cacatcagca cggacgtcgc
      721 gtcggcaccg ctgctctggg tgctcccgct cgccctctat ctcctcacat tcgtgatcgt
      781 ctttcgcgag cgcgctctca tccccatgcg gctgttgctg atcgtccatc tggcggcggt
      841 ggtcgccgca cttctgcagc tctcgcaaac caagcacgat ggctggttca tctccagtgc
      901 cacgggcgtc gtcgtattct tcacgtcggc gctcgtcgcg caccgcacgc tttacgacct
      961 gcgccccgcc ccgcgctatc tgaccgagtt ctacttatgg atgtcgctcg gcggcgtgct
     1021 tgggggcctg ttcgctgcgt tggtcgctcc gcacctcttc tccgaggtct tcgagtaccc
     1081 gctgctgctc gcgctgacca tggcctgccg ccccggagca ctttccatct cgctccgaga
     1141 ccgggaagag ctgttgcgga tctggctggt cgtggctggc ggagtgctcg caatctattg
     1201 ggcaccatgg ctcgtcgagc agctttcaga catcagcccc ggcggcatcg tcggcaccgc
     1261 aatcgacaaa ttgctcgagt ggggtaccgc ggcggccatt gtcctcgcct tcgccatcct
     1321 gctcgtggtg tcgctgcggc atcccccgcg gcaggtcgcc atcgcgatgc tgatgttcct
     1381 gacggtggtc acgctgccct cgggcgtgaa gcgcggcgat gctcagcgca gctacttcgg
     1441 cgtctaccgc gtctcactgt cgagcgacgg caacttccac gtcctgaccc acggcaccac
     1501 actgcatgga gcgcaacgga tccgcgacag cgagggcgcc tacaaggacg acctcacgcc
     1561 gaccacctac tatcacccga agagcccgat ggcgcgcacc atcgccgaag tgcgtgggcg
     1621 tctggcgtgg cagggccgca ggggccgata cggcgtgatc gggctcggca ccggatcact
     1681 cgcctgctac gcccggcagg gcgagcgttg gcgcttcttc gagatcgacc ctctcgtggt
     1741 gaacattgcc agcacgccgg agcaattcag cttcctgcat tactgccagc ccgagccgga
     1801 catcgtgatc ggcgatgcgc gcctgacgat cgccaaggag ccggacgcga gcttcgatct
     1861 catcatcgtc gatgccttca cgtcggatgc cgtgcccgtg cacctgatga cggcagaggc
     1921 tctgcggctc tatgcgtcca agctcacggc cgatggcatc gttctgttgc acatctcgaa
     1981 ccgctacctc gatctcgatt ccgtcctcgc cgccacctct ccgctggctc cggagctcca
     2041 cggcctcatc ctttccgatg acgatgccga cggcagctac gccagctcca cttcaacggt
     2101 ggcggtcttt tccaaggacg aaaccgttat caatggctac cgcgactggc cggaggctta
     2161 tgaattggag gcaaacggac ttcgcggctg gaccgacgat ttctcggata ttctcgggcc
     2221 attgcttgcg aagctgcggt agggccaggc agacccggat cgcacctcac tgcctccgct
     2281 tgaacgccgc cgacgccacg agcccggccc ccacggcgat cgtcaccgtg aaaatgccgt
     2341 agagcagcgg ctggttgaag gcgaagttgt gcagcaggag ctcgagcccc tcgcgctcga
     2401 ggcgcacgcg cgccgtatag gtgctgatga tctggccgtc gcgaaagaga tagacgcgca
     2461 cgtcgagcgg acccaccggc acgttggcgg gcagattgac cgaggcccgg aacagactgc
     2521 gcccgatgaa gacgacgccg tactcctcct ggacgtaaag gccgtccttc tgcttcagcc
     2581 gcacgacagc ctcggcgaac tcctgcagct ccgcggtggt gacgccggtt tcccaacctt
     2641 gcacgggcgt catccggacg tactcgaatc cgatgtcgtt ctcccgcagc accagcgggt
     2701 tggtgatgtc gtccagcggc cgcgtggaaa gaatggcgta gtagctcggt acgctctcga
     2761 aggtgatcga gtgggtgttg atccagatcc ccgcaacgtt gctcttgcgg cgggcgacga
     2821 ggcgcgtcgg ggtaccctcg agcacgatga ccacgtcgta tagcccggac tccgcggtcg
     2881 tctgacggct gtggtcgacg gctccgaaca ccacgatctc ggtgccggta aagctggagg
     2941 tgaccgagac gctccgcgag gaaacatccg cctgcaccgt ctcacgcggt ccggcttccg
     3001 cggggacggg ctgcggcggc ctcgtctcgg cctgtgcaac gggtgcgcta cgcaccggct
     3061 gcttcggcgc gacagcaggc gagcggaccg gcggctcggg ccccggacgg cgctgcgctt
     3121 cggcgatctc gaccccgatc agcagcgccg ctgctacgat gatcaggacg gcgcccagga
     3181 aacctctcag aactcgccag ttaatcttca tcagtggctc ccgatcgcgt cactgatgga
     3241 atactcgtcg ctcggcgccg taaccaggcc ccagccgata cggacgcaca ccagcagcac
     3301 cagccccgcc agcaaaaatc gcagctgctc acctttcagc ttctggcctg ccgccgcgcc
     3361 cagttgggcg ccaacgaccc caccgatcat cagcagcagc gccaaggtca catccaccga
     3421 tttgttctgt gcggcgtgca gaatggtcgt ggccccggtc acgaaaacga tctggaagag
     3481 tgaggtgccg acgacgacgt tggtcggcac ccgcaagaga tagatcatgg ccggcaccat
     3541 gatgaacccg ccacccacgc ccatgatggc accaagaaag ccgaccagca ttccaattgc
     3601 aaacggcgga ataatgctga tgtaaagttt cgagcggtgg aagcgcattc tgagtggcat
     3661 tttgtgcacc cagttgtgct ggccaggccg ccgcatcgac atcacctttc catgccgcgc
     3721 ctgccgcatt gccttggcgc tttcgatgag catcagcacg ccaatgatgc cgaggaaggt
     3781 gacgtaggcg atcgaaacga agaggtcgaa ttgcccgatc tgccgcagca gcttcacggc
     3841 ctcgactccc gctgccgccc cgacgatgcc gccggacagc agcaccgttc ccagcttgac
     3901 gtcgacattg ttgcgccgcc attgcgcaat ggcgccggat accgacgagg cgaggatctg
     3961 agccgcttcg gtgccgacgg caatggctgc cggaatgccg gaaaaaatga gcagcggcgt
     4021 catcaggaag ccgccgccga ccccgaacat tccggacaaa aagccgaccg cgccgcccat
     4081 gccgagaaag gcgaaaacat ttacggacat ctcggcaatg gggagataaa ggttcatgac
     4141 tgagccgaag cctgccccag cgtagaaagc tcctgcaccc ggggccacct tatgcaccgg
     4201 gagagcctaa ctcagccgaa tggctttttc ttgatgagtg tacgagcgcc aagtttaagc
     4261 cagtgctgaa ccgcggggtc aaccgtattt cgctgggcaa cccaacgatc cgggctccgg
     4321 cccacataga tctctcacac tcgaaaagtc ggatagggaa ggtgaccagc acacctcgtc
     4381 cagcaccgcc ttgaggacga cggcgctatt gcgaacctca cccttttcgc aaaatgcggc
     4441 atggccggcc tccgctcgcg acttcgcgca gacgcgcggg aaccggctgg caattcgccg
     4501 cgctcgactt ccctccgcgg gcaacgctcg aagtcctttc actttgtcgg attggcaaca
     4561 aacgcccagc gcagctcgtg gctcgggcgc aatctctcgt gcccactcgt cgatggccgc
     4621 cttttcccga ttgaggccgc ggggccagag gcgatcgacc aaaacgcggt agccatcgtc
     4681 cggacagggg gctcatagag accctgcttg catacgatcg tgctcaaccc ggtgacacct
     4741 gaaggcatcc ggcagcaacg gcgcgatcag ccttgtactt cccgctgctt ccagacctcc
     4801 cctgcggcgc gcacgttgtt caccagcggc tcggccggct ggggatgcca ggactgcacc
     4861 agcccttctg ccgccttgag ctcattcgcg ttcagcaacc acttcacgcg atcccggcgg
     4921 cgcatggcgt ccttgtcgcc gctgcgcgca gcgagcgcaa accatttgta ggcctgcttg
     4981 aggtcgcgat cgacgccgcg gccggtctcg tagaggacgg cgagattgta ctggctgtcg
     5041 accaggcccc gctctgccgc ctcggagaac caatgggccg ccgtcttgta gtccgcggga
     5101 tcgccgctgg cactcagaac ggcgaagttg tgcatggcct tcaagttgcc cttatccgct
     5161 gcgctccggt accaggccat ggcccggccg cgatctgccg ccacgccgag gccgcgctca
     5221 tagagcgtgc ccagccggta ctgggccggc gcgaagccgc gcgaggcaga gcgctggtac
     5281 cagatcaccg cctccttgag attgcgctcg acgccccggc cctcgtcgag gcgtacggcc
     5341 acctcgaact cggcggacgg gtcgccattg gcggcggcaa gacgcagaga gagcggcccc
     5401 acggcagccg gcggcatcat gcgcctggcc ggcacgggtg gcacagcctg cgctggctgc
     5461 ggcgtgacgc ttcttgtatc cggactgaag gctgcgcgcg cgggtgccgc tgagatcacc
     5521 ggcggaagag atccgttgtc gatgatgatg ccgcccggga gcgtcgttac actgcggggc
     5581 cctccagtga ggctgtcgtc ctctcccgga ccgctgcggg cgccgggcgg atcgatgatc
     5641 acttccggcg tggtggtgcc cgcgggagcg cttgaggagc caccgtcgct caggccgcca
     5701 gcggccttgc cttgaccagc cccggccggc gcagtcgctg cggtgccgcc cttcacgagt
     5761 gccccgcgga ccaagcccca cggcttcgcc agatcgccct tgaagatgct gtaggtcgta
     5821 accccgatgc cgaccgccag agcgacggca agggtcgcca ccaacagacc tgtcaccggg
     5881 cgcgcaccgg ctgcacgacg attgccggcc tgctgcaggc ggaccttcgc ctgcgacacg
     5941 tcagcagttt cttccatcgt ctgtgcagag gccttgcgcg ccgcgcggcg ggcggccgcg
     6001 agaaaatctt cgcggctcgc gcggagcggg ctcgattcgg tgccattctc cgccttcacc
     6061 acggcggtcg cctcctcgcg tgaggcggcc tcggcgctcg cttgatcggc cctctcgatc
     6121 ctcacagttg atcgtaattg ctccagctct gcctcgattc ccggctgttc ggaagcgggt
     6181 ttcgactcgc gcggccactc gcgggggaaa acgctgctga ccgtcggctg atcgttttgc
     6241 tgcaggccat aggccgaatc ggctgctgca agaccatcgc tctcgtgacg ccagtcggcc
     6301 tctattgccc ggcgggcggg aggtgccggg gtgtgagcgc cctccagcgc ctccatgcgg
     6361 tcgaggacgt tcagcatggc ctcctggatc gcctcgagcg cgctggccgt ctgctcgcct
     6421 tcccgcctct gagcgttgac gaactgctcg atgatgtcgt gcagctcgcc caaacggtcc
     6481 aaccccgatc cgaagtcgcc gcgggccgtt tcgcgcgcag acagccgctc ggctacggct
     6541 tcggccagcg ccgccagtcg tggctccgaa aggatgcgct gatcgaacag ctgggcgagc
     6601 cgctcgtccg agaggtggtc gatgagccgc gtcaggttgt gttcgatctc gtccagccgg
     6661 gcgagccggt gctgcgctcg gtccacgtgg cgcgccagct cgctgatgtg ggcctcgacc
     6721 gccctcaact tttccacatc ggcccgcgtc gcgagctcac tgatggcaga gcccagcttt
     6781 tggtcgaagc tgtcgagccg cgccctcagt gccgtaacgg acgaattcgt ccgcagctcg
     6841 accagcgcct cttcgacgcg gtcggcgagc tcagcgaacc gctgctcgaa gtgatctccg
     6901 gttgtgccgt tcccttggcc gtcgctccgc tcaggggcta gcgcatggta cggttgcccc
     6961 gcgtgctgct gcgtgtgtga attttggaaa gattgaccac gaggcttgtc gccgaaatgc
     7021 tccggctcgt agctgcgcgc cagcgcctcg gccgaagccg tgtcccagac accgtgatcg
     7081 ccggcatcgg gaatatctgg atacggatcg gccatggccg aggcgtagct cggccgaggt
     7141 ctggcggctt ccaagctttc gaagtgagag gggccggctg ccgatctcag gggcggcggc
     7201 ggatccgttc tgctgcgcga ccagatcggc tccgagtaac gggcattcga ctcgccaggc
     7261 ttgggaccga acgctggatc gtcatggctg gagcccggcc aatacgtgtg gcgatcgagc
     7321 tcgctggcac gcgatggctg cgccgtgccg ccatggtcgt ccgcggccac tcgtttggtg
     7381 agcgtcgaga tgctctcctc gatacgggcg aaggctgctg cgagctccgg ggtaatagag
     7441 gtgcggacgg attgggcttc gtcggcaaga tgcgcgaggc gcgattgcat ctcgccaagg
     7501 gctgcttgtt ggcgttcatc ggcattggcg atgtactctg caatacgctg caacagcagt
     7561 ttgagctcgc cggcgccaca gacggaataa ctcgatggtg ggctcacctc gtgacgaaag
     7621 ccgcccgtcg aaccttgatt attattaaag gacatacctt ttccccaatg aggtcgcagc
     7681 gatcgcgagt gccctcgatc ggcccccatc ggagaagtgg cgacatgccc gcccttgcct
     7741 gtgccgctca ggagcgatcg tggggtccga aacgcggcac gatctggacc atcgcccaaa
     7801 cactggcagg ctcgagtaaa ggttcagtta acgaaggtta ccaactacag taattctcca
     7861 gtcgaacgag gatttcctgg ccgcaaactg gccgcaactc tctggctcat gcgggcaaga
     7921 gccgaggatc ggccaaagct tgatcttgca agcgcgcttg aatcggctgc tttcgcgcgc
     7981 aacatgaagc aaatgccagc tgctcttgcc gcgtgcagca atgaacggat cagtcatagg
     8041 agtagcagat gcgagccctc gtcattgcca tccctgcaat tgttgcaatc gggctcagcg
     8101 cagccaatgt ggcgagccag gagcggcagg gccgctatgt catgaacccc gtcgataacg
     8161 gcttcgtgcg gctcgataca gaaacgggtg ccatgtccgt gtgcacgcag cggaacggca
     8221 ggtgggtttg cgaattgatg gaagacgagg caaaggccct gcaagaggaa atcgagcggc
     8281 tgaaggccga ggtgaaacgc ctcaaggatc aggccgcgct cgccgagccg agccccgacc
     8341 gtgcatatcc atcagagcgc cctgagagga gcttcgagct gccgacggaa gaggaagtcg
     8401 acaaggcgct cgattacttc gagaacatct tccgcaagtt ccgagagcgg atcgaaaggt
     8461 tcgagaagga acagcgcggg gcagaaggcc ggaaggatgg cacccagctt tgatcaacag
     8521 ccccgatggc cgaggtgcat gtgctgaagc aggcgagcac gacactttcc atctccaccc
     8581 gaggtccggg cttcatcgac ctcaccggca ggattgagga ttgggtggac aggatcggcg
     8641 cgcgcgaagg cctgcttacg gccttcgtcc gacacacctc agcgtcgctg acgatccagg
     8701 agaatgccga tcctgatgtg cagagggatc tgctcgacgc ccttgaccgg ctggcgccac
     8761 ggtcacacgc atacaggcac ggcatcgaag gtccggacga catgccggcc catatcaggt
     8821 cgatgctgac cacgacctcc atcggaatcc cgatcgtcga cggccggccg gtgttcggca
     8881 cctggcaggc gctctatctg atcgagcatc gcgatgcgcc gcaccggcgc gaggtcgtcc
     8941 tgcagttcat cggcgaggtg cggtcagctt gagcgataaa ggaccgatat ccacttgagc
     9001 gccgccggct tgctcccgcc gactgacggc cgtatcgaga acacgcaaag cgcacacgcg
     9061 gacaagggct gatgctgata cttcggatgc tgagcgggat tttcctactt gccgccgccc
     9121 tcgccttgat tgcggaagtg acgaacgcac agctcggcat cccaggcgca ccattgacgc
     9181 cagtgtacaa acagctcgcc gatgccgccc cggcggcgtt gaaagcgatg gagcaggcgg
     9241 tgagcaaact gcatcccttg ctgtgggatc ctgtgctcct gtccatcctg cggctacccg
     9301 cctggatcag cctcggcagc atcggcgcgg tgcttgggat ccttggccgc cggcgctatc
     9361 agcggatccg cgtgttcacc aactgaaggc cgcttgagct atcgccgcgg cgcaccggcc
     9421 tgctgaacct cgggagacga cttcatcagg ctttggacga gctgatgggc ccgctcaccg
     9481 agatcgaggt ccgatttcga gatgtcgagc atgaggccgc ccgatacgag caggaacagg
     9541 caaccgcaaa gtaccagcat cgcagtgttg tggcgccggt tgagcgcggc gatctctgct
     9601 tccgaaaccg gattggcgcc gtcgtcggtc gcccgaattc cgcgcagctt cttgtggacc
     9661 tgccgccgca ccgtcccttg gtcgatgacc tccaggccgg cgcgccgcgc gatttcggcg
     9721 gcgatctgcg ggaccggcat caccggatcg acgttggcct cgttgacata ccccagcggg
     9781 atcttgtcgc cgcctttcgt cacgatccgg gccccgcgca acatgaccgg cgcgatgagg
     9841 ccgccgtaca cctcgcgccg ggtctccacg gcctggatct cggagtaggg cacctcgcgg
     9901 gttttgtaga acagcttcgg aatggccatg ccgcgggccg ccggcaatgt cagccggacg
     9961 gcgctctcgc caatcgcaac gcgggcgcgg atcgaatgga tgagctcgaa gagcaccagc
    10021 agcatgatca gcgcaaaggc gacggcgaag atcgtgaagc ccaccatatt cggccaaagc
    10081 tgatgcatcg cccgctggat catcatggcg ggcaggctga cgaagaacgg cagaaggatc
    10141 aggaagatga aggacaggat cgtcttccgc acagtgctcg cttgatagac gatcctgccg
    10201 gagctggtgc cggtggtggg ctggggtgtt ttcgattgcg ctggcttctc gagctctctg
    10261 gaggcggtgg ccgcaagcga cgagctcagg gccttatcgg acgctgggtt ttcagattgc
    10321 gcagacgagt gccgctgcgg cgtatccgct gtcgttgccg tggcattgga ggtctctgcc
    10381 tgcgtcacat ccgccggcgg ctgtttgggc tgagcgtcag cttcggttgt cgaggattgg
    10441 acggaagccg ggggcccctt acctgcgtcg tcaccctcca ccatgttccc ctcccgtgca
    10501 gacgagcgct tgagagcggg gccgtcgcgg cgaagtgatc gcccggcggt gttccccgcg
    10561 ctcggcttac tatatcaggg acgaagccgc gcgtactgcc caaccaatcg caccgaggtg
    10621 ctcccatgct atttcctcga aagaagacgc agatgcccac tcctgagacg gccctcaaag
    10681 ggcgtccgac accgatcccg acggccgaaa cccatttcgt caacgggcgg ccgctgaagg
    10741 ggccctatcc cgacggctat gaaaaggcga tcttcggact tggctgcttc tggggtgcag
    10801 agcgcaagtt ctggcaactt ggcgatggaa tctggatcac cgcctcaggc tacgccggcg
    10861 gctacacgcc aaatccgacg tatgaagagg tgtgctccgg cggcaccggg catgccgagg
    10921 tcgtgctcgt cgtctatgac ccgaaaaaga tcagctacga ggagctgctg aagacgttct
    10981 gggagagcca cgatccgacc cagggcatgc gccagggcaa cgatgtcgga tcgcagtacc
    11041 gctcggccat tttcacaacc tcgcccgagc agttcaccgc cgccgaggct tcgaaaaagg
    11101 cttaccagcg ggtgctctcg gccaatggct atggtcccat caccacggag atccgtgccg
    11161 cgccggagtt ctacttcgca gaggactatc atcagcagta cctggccaag gttccgcatg
    11221 gctattgcgg gctcggcggc accggcctgt cctgcccgat cggaacgggc gttgaggtcg
    11281 cgagccgata gcgggccgga aagcccgccc cgtttggcac atcgctattg ctgctgcgcc
    11341 ttcgttctgg tcgcgcgtac gggaacagga accttgagcg gtgcgcttgc cgtagccgcg
    11401 gcgggtgcat ccgccgaaag gacggcacgg cactgatgcg gcaactgcgc cagcgtcagc
    11461 ggcggcttcg gcttcgaggg cttcttaggc ttgggcggag ccgcaatcag ggcgagccag
    11521 tcgctcagct ccttgccgca gccatcgccc ggaggaggcg ccgcctgatc tttgcatgtc
    11581 gcgctgcccg cagggcagcc catgcggaca tggaagtggt aatggtgccc ccagtagggc
    11641 cgcacctttc gcagccagct ccggtccgat ccgattttgt ccgccccctc gcacaaggct
    11701 ttcttgatcg cggggtgcac gaaaatgcgg tccacctcgg gataggaagc ggcgcgcctg
    11761 atgatgcgta cgtggctgtc cgtccagata tttgggttga cgctcagctt gtcgggaccc
    11821 agcatggaaa ctgccgacat ttcctcacgc tctttctcgg tgagacgccg gttcggcatg
    11881 ggcgtgaacc agatgtccgc atcgagcccg atctggtggc tggcgtgccc cgtcagcatc
    11941 ggcccgccac gcggctgcga gatgtcccct accagcagcc ccggccagcc gtcatacttc
    12001 tgagcctccg tcgccagccg ttcgacgagc gcgacgagcc tcggatggcc ccagttgcga
    12061 ttgcgggaaa gccgcatcgc ctgccaagcc gggccgtcta tggcgagctc cttgccgccg
    12121 gccaggcagc cgcgcgcgta aaagccgatc gcctcgggct tgagctcagc cggtccggac
    12181 accttgccga agagctgctt tgcaggcacg gacgcatctt gtgcgccggc cgaccctaag
    12241 ggtgctacag ccaagatggc ggacaatagt gcggccactg tgcgcatgaa ttatcccgaa
    12301 ttggtacaaa gatggcccgt tctaaactaa ttgagggccg aaatgaggcc aacatggatc
    12361 cggcggcgtg caagcaacag ccaagatctg ctctgggctg cgaagtacaa tgactgaatt
    12421 tctcgatacg ggcgtccccg attcacccgt gcggctcgtc ctggcacacg gggcgggcgg
    12481 agcgatgaca agctcgttcc tggagactat tgcggcactg ctggccaacc gcggcatcgc
    12541 cgtcacccgc tttgagttcg cctacatggc cgcccggcgc acgggcggca aacggcgccc
    12601 gccgcccagg gcggaacttc tggtgcccga atatcgctcg gccgtcgagg aggtccggca
    12661 gcggctcgcg aaaggccaga agctgctgat cggcggcaag tcgatgggcg gccgcgtggc
    12721 gagcctgctg gcggacgaac tgttcacgga aggccgtatt tcgggcctgg tctgcctcgg
    12781 ctatccattc cacccgcccg gcaaacccga ccaattgcgg acggagcatc tggtcgacct
    12841 gaaaactccg gcgctcatcg tgcagggtga gcgcgatccc ttcggtagcc gcgcagaggt
    12901 ggagcaaatg gccctgtcgc ccagcattcg cgtccattgg gccggcgatg gcgaccacga
    12961 cctgggcccg cgtggcggca aggggttcac gcgacgcggc aacctcgacg cggccgcgga
    13021 cgccgtagca aacttcgcga aatctctcga tcaggcagcc gccggaaccg gctccggatc
    13081 aggcaagcgc cgcggcagca gccacacggc gatcaggatg gcgcctgctg cccccgcgag
    13141 cacctggaac aggcggtcga agccccagcc ctgatggatc caggcgatca gcggcaacgc
    13201 agcggccagc actgcgaagc tcacgatgta tcggacgcca tagatggaag cgcgcatgtc
    13261 cgaccgcgcc agcttgccga tcatgtagtc gttgatcgga atctgcccga acgcgcccag
    13321 catgaaggcc agcgccacga ccaacgccgc ccaatccgcg agacccggca tgagcgagaa
    13381 gaacacgagc tggatggtgg cagcaccgat gaacaccgtc cgcggcccga gcctgtcgag
    13441 cagacggcca acgatgatct gcgcaatcga cgcaacggca aacaccagaa atgcgagcca
    13501 cccgatgatg gtggcagagc ccgcaatgcc cgtcaggcgc tcgtcgaaca ccttgggcaa
    13561 agcgaaggtc gtcgactgaa agacgatgcc ggagactgcc gtcgtgaaaa agatgatgac
    13621 cgacagccgg aggagcagcg ccttgagatc cggcggaagg ggcgccgccg cccccttcga
    13681 tttgccccgc ccattgccgg ccgctggacg catctcggca tggaacagcg caatgtagcc
    13741 gagaccgatc aagatcgaga ccagcccggg aacgaagaag gcgagccgcc agccaccgtt
    13801 gtcgatgaaa aatccggtga tcagcgcggc actcgccacg ccgaggttgc cccagacgcc
    13861 attgatgccg atggccatcc cggttccggt tctagaggcg cgctcgacga gtagcgcaag
    13921 cccaacgggg tgatagatgg ccgcgaacac gccaaccacg aacaggccaa tgcccacttc
    13981 gatgggtgta cgtgcaaagc ccgtcgcgat cgccgccaat ccgatgccga cgaagaacac
    14041 cgccatcatg ccttcccggc tccacttgtc ggcgagccag ccggcgggca gcgaaaatac
    14101 gccgaaggcg atgaacccgg gcgtcgcgta cgggatcagc tcggagtagc tgagccccca
    14161 ctcgcgcgta agcgccaagg ctgccaccgt ggcgaaaacc agcgtgagca gatgatcgac
    14221 aaaatggccg acgttgagga acacgaagtg cacgcggtca cgggtcgtgg cgtgtgtcat
    14281 cggtgcgctc cttcggttcc cccgtccaaa tcttgcatca ttcatagcct tcggcaatgg
    14341 aggcggcgat cctgtcctcc agatccacgc gatcggcggc gccgctcgga tgcgccttgc
    14401 tcggcatgag ccgccggaag tgcagccagc gctcgcgcgc caccgcctcg aggcgctcca
    14461 gctcggagag tgggtcgggg tggtcgtcgg cccggatgtc gagaagcgga tactcctcct
    14521 gatcgtggat cagcaggcaa gctgattgct tcccgcggct gtcgccgccg gctgcctccc
    14581 cggccttcat ggccttgatc aaacggcggg cgaagggcgt ttgcaggttg gcttcgtagg
    14641 tccggagcgt ggcatccacg acctccggtc cggccagcat gttaccggcc acggagagat
    14701 gcggaccgac gcggtggccg caccagtcga tacaggcggt gccggtgaat gcggcgaacc
    14761 ggccctcgcg atccatgacg tgaacctgac ggtgatcacg cccgtcgtcc ccgccggtca
    14821 aaaacttgac cgtgtcctcg gcggttgcac ctgcctgcag caatgccaat ccttgggggc
    14881 cgtaaaatgg attgacgaac gcctgcgtgg cgatcgcgcc cactcccgtc ttgatatacg
    14941 gcaccaccgc gccgatggcg aaaaagcggc tcgcggcgat gatcccaacc cgccctgttg
    15001 cctcgtcacg ggcgatgatc gaccaggtca ttggcgtgcc tcacgtgtga aatccgcgaa
    15061 ggagcaagtg tacctgtacc ttcgatcgcg ggcctaatcg tgacccattt ccctgcggcc
    15121 tgtcaggaac aagtcgaaag tttcgagaac atatccgaga agaaggcgtg acagctcgag
    15181 gaggagctgg gcttggagat ggacgtttcg ccggctgcca ctccgggtca agagctgctg
    15241 acattcagga ctgctgttcc acccagacgg acagatcatt ctgccaatca tctgcccctt
    15301 aaggtgaagc cggagaacgc ctcagcacac gccccggctc tgcatgagcc ggcggcgcaa
    15361 catgatcaaa tagaacagga tgaacatgta gtagaacgtc tctgccgcct cgctcggccg
    15421 cggcagcagc tctggcagga gttcattctt ctgcaagctg accaacatgt tgaagccgac
    15481 tgccgtcaat gccacgggga cgatcgctcg gggcggggtc agcacgacaa tgattggtat
    15541 gccagcgacc cactcccgtg cccgccggga aagcggcagc acgagcccgc cgacgacgat
    15601 agcgatctgg aggacctttt gcggcagctg attgaacagc ggtgacgtgt tgtggaggtt
    15661 ggtttcgttc tgccggttga actcccccca aaactcgggc gtgtcccaat gaaagaacca
    15721 ttgcccccag ctatgctctt ctccggcaat gtagaagcat gatagcgcga acgccacgat
    15781 ggcggcccga agcagcggcc aagatttgac cagcgggctc gagaacaggc cgaggcaaag
    15841 aaaaaatcct gctaggggca ggaagaagtg cgagagctcc agcacaccat aggcttccgg
    15901 cagaatgcgc tcgcgataaa agtccggcgc catggcgctc gttgccagaa tcgcagcggc
    15961 ggtgacaagg ggtactccga gccaccacca agcctctgac cagctcgggc attgaggttc
    16021 gacacgtacg caatacgact tcaccacgtt gcctttcttt tgggtgaatg actcgaggtc
    16081 agaccgcctc tagcggctgc ccgactaacg tcgagacaca gcagggcatc tgcgggattg
    16141 atgcattcta gctcgccccc atccgggcgg cagtccgcca gttacttcga tgccacagtt
    16201 caaccgcgac cgtgggaatc aggcaccaca tcagaggccc aatctgacgt cttggcagga
    16261 gctcgcacga ggtgcgctac aacttcggcc agcacgtcga agtcgaacgg cttcaggagg
    16321 gtcggagcat tcttgaactg cgggtcaatg gtctcagtcc catgccccgt cgcgaaggcg
    16381 tactgaatcc cacgctgctc gagagccctg gcgatcggat acgacggccc gtccctgacc
    16441 gtgatgtcga ggatggcgca tctcagcggc ggcccgcctg cgataagcgc aagcgccgct
    16501 tcattgctgg ccgccggccc tacggtctcg taaccgagct cggcgaccca ctctcggagc
    16561 atttccgcga tcagtggctc gtcttcgact atcagaatac gcttcgacaa ctgcatccaa
    16621 ccaagttttt cggtttgcca gggggtctca cggcgaagca tagcctgaac gtcgcgccag
    16681 tcctagtctc cgggtgccga agttggccgg actgcttcgg tcgagagcgg gcgctcgaat
    16741 caaaagcgac accagagtca tatgttgcta gtgtaatccg gattgagaga ttgcttgagg
    16801 cacatgcggt gagcgccgcg taattctcga tcatcaccat agaacttcgg gtggggaaat
    16861 gccggtatct tggctgcaat cggttaagat cagaacaggc gctctctccg ggatcggact
    16921 ggatgaggcg gaccgcctgc cccgatggac catcgcgatg tttgcagcga gcttcgtcac
    16981 gctcctggtc gcaggtatct cagccggcct gatcgcgacc cgctctcaaa gagtcgagaa
    17041 ggtcgccgag cgcacgctgc aggttcatcg cctgaccgaa cacgtgatta ccatgctcca
    17101 actcgcagaa acgagtcagc gcgggtacct gctgaccggc gacgaggaat ttctgaagcc
    17161 ttataggacg gccgcatccg aactcactcc cgagtgggaa aaactgaagc agctcagcca
    17221 gacgaatggc tccctcgatg tgcagcttgc cgagctgtcc gcgcttatcg atcgccgact
    17281 gagcggactt caggaagcca tcaccttact ccagcgaggc gacacggaac gcgctcgtga
    17341 tcctaagcgg ctgaattcgg gtcaggcgca gctgctggag atccgggagc gcctcacccg
    17401 cctttcggac gccgaagaag cgcagatgat caggttccag gccgaagccg ctgagctgcg
    17461 cgagactctg atgcctctgg tggcgatcag cctcgccgcg accgttgtgc ttgcgctact
    17521 cgtcggccgt gcgatcaccc attacatcag tcggctggtg gcccgtacgg ccgaactcga
    17581 gcgcgaagcg cggctgaggc gcgagacaga ggacacgttg cgccagtctc aaaagatgga
    17641 agccatcggg cagctcacgg gcggggtggc tcacgatttc aataatctgt tgacagtcat
    17701 tctcggcaat ctcgacacgg tcc
//
LOCUS       DASVDU010000120         3724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1576110, whole genome shotgun sequence.
ACCESSION   DASVDU010000120 DASVDU010000000
VERSION     DASVDU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576110"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1165
                     /gene="hemH"
                     /locus_tag="VF226_07715"
     CDS             98..1165
                     /gene="hemH"
                     /locus_tag="VF226_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7073911.1"
                     /translation="MNELPKATTLSKEQTGRSILPADHPEVRHGRVGVLLLNLGTPDA
                     TDYWSMRRYLKEFLSDRRVIEKPRLIWWPILNLIILTKRPHSKGRDYDTIWNKEKDEG
                     PLKTITRNQAEKLAAALDNPRIVVDWAMRYANPTTESRIKALQEQGCDRILLVPLYPQ
                     YAAPTTATACDQAFRALMKMRWQPYVRVAPDWHDDPVYIEALARSMEAHLARIDFKPE
                     AIIATFHGMPEDYLHKGDPYYCQCEKTSRLLRERMGWPKESWHTTYQSRFGNDVWLQP
                     YTDETVKRLAQSGVKKLLLIAPGFAADCLETTEELEVENRDIFLENGGEQFTYVPALN
                     ESPDGMRVIEHVVRRELMGWV"
     gene            1280..1726
                     /locus_tag="VF226_07720"
     CDS             1280..1726
                     /locus_tag="VF226_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7073912.1"
                     /translation="MYKHILIATDGSELADKGVEHGLALASALGSQVSVLTVSEPLSP
                     EAIQAARAGGIEDPVGRYDQQIDAIMKERFASIEKRAEKHNIKVEFVHEVDDSPAEAI
                     VRFAKLRNCDLIVMSSHGRRGARRLILGSQTAEVVTTTTIPVLVIR"
     gene            complement(1796..3022)
                     /locus_tag="VF226_07725"
     CDS             complement(1796..3022)
                     /locus_tag="VF226_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7073913.1"
                     /translation="MGMLRSDIVISGGSYAGLTLALALAKTLGPEIAITVIDRQVVPT
                     GRDVRAFAISAGPRHMLDVLGIWPKVRDEAQEVLEIQLTDSPLEAGVRPVLLTYDNTL
                     ENGEPASFIVPNEALATALENAADAEPAIQILRPAEATGFTEGDPFLRVHLADGRSIE
                     TSLLVAADGRRSRLREAAGIKCIGWSYDQIGIVTQVAHELPHNGRAIQHFLPGGPFAI
                     LPLRGNRSCITWSEDAVRAREILAYDDEAFLAEVDKRFAGRYGRLTLAGPRASWPLEM
                     HLARRYVGHGFALVGDTAHGVHPIAGQGLNLAFRDIAALTECVAEAVRVGLTPGDGSA
                     LERYEQWRRFDSGVSAAAFDALNRLFSSDAALVRSAREAGLALVDTMPTLKRMFVAEA
                     AGLSGEVPRLLRGELA"
     gene            3120..>3724
                     /locus_tag="VF226_07730"
     CDS             3120..>3724
                     /locus_tag="VF226_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX7073914.1"
                     /translation="MPSERSKPTSALAALLSILDLEQLEQNLFRGQSPRQGWQRVYGG
                     QVLGQALVAAVRTVSEPRTAHSMHGYFLLGGDPNHPIIYEVERIRDGTSFTTRSVKAI
                     QHGRAIFAMSVSFHKEEPGFEHQSQMPDVPPPEDLPSEHQLRDLLVAKLPENMRSYWE
                     RERPIEMRPVDISRYLKREKAPPVQYVWMRANGALPDDPKL"
BASE COUNT          699 a         1220 c         1172 g          633 t
ORIGIN      
        1 gcgcccctgc gtcatgaggc aggctggcgc cgcgctgcat tatggccgaa aaggtccgcg
       61 agtacgtccc ccgctgatgt ccggaggtca cgcgctgatg aacgagctgc ccaaggccac
      121 gaccctgagc aaggagcaga ccggccgctc aatcctaccg gccgatcacc cagaggtacg
      181 gcatggtcgc gttggcgtgc tcctgctcaa ccttggcacg cccgacgcta ccgactactg
      241 gtcgatgcgg cgctacctga aagagttcct gtcggaccgc cgggtgatcg agaaaccgcg
      301 tctgatctgg tggccgatcc tcaacctcat catcctgacg aaacgtcccc actccaaggg
      361 ccgggactac gacacgatct ggaataagga gaaggacgag ggtccgctga agaccataac
      421 ccgcaatcag gcggaaaagc ttgccgcggc gctcgacaac ccgcgcatcg tcgtcgattg
      481 ggcgatgcgg tatgcgaacc cgacgaccga aagccgcatc aaggcgctgc aggagcaggg
      541 gtgcgaccgc attctgctcg ttccgctcta tccgcaatat gcggcgccga cgactgcgac
      601 agcctgcgac caggccttcc gcgcgctcat gaagatgcgc tggcagccct acgtgcgcgt
      661 tgcacccgat tggcacgacg atccggtcta catcgaggcc ctggcgcgct cgatggaggc
      721 acatttggcc cggatcgatt tcaagcccga ggccataatc gcgacgttcc acggaatgcc
      781 ggaggactat ctgcacaagg gcgaccccta ctactgccag tgcgaaaaga cctcgcgcct
      841 gctgcgcgag agaatgggat ggccgaagga gagttggcac acgacgtatc agtcgcggtt
      901 cggcaatgac gtctggctgc agccctatac ggacgagacc gtcaaacggc ttgctcagtc
      961 aggtgtgaag aagctgctgc tgatcgcgcc gggcttcgcc gcggattgcc tggagacgac
     1021 cgaggagctc gaggtcgaga accgcgacat tttcctggag aacggcggcg agcagttcac
     1081 ctacgtgccg gccctcaacg agagcccgga cggcatgcgc gtcatcgagc acgtcgtgcg
     1141 acgcgagctg atgggctggg tgtgatgcct ggcctgttac tttcagaatt gatctgaaac
     1201 atggactgac gaggcggacc ccgccaccct tcgatcgtgc ccatatttct tgtgggcgcg
     1261 atccagacgg aggttaccga tgtacaagca cattctgatt gcgacggacg gctccgagct
     1321 ggccgacaag ggtgtggagc acgggcttgc actcgccagc gctcttggct cacaggtttc
     1381 cgttcttacc gtctctgagc cgctctcgcc ggaagcgatc caagccgcac gtgccggcgg
     1441 catcgaggac cccgtcgggc gctacgacca acagatcgat gccatcatga aggagcgctt
     1501 cgcttctatc gagaagcgcg ccgaaaagca taacatcaag gtcgagtttg ttcacgaggt
     1561 tgacgattcg cctgctgaag cgatcgtcag gttcgcaaag ctgaggaatt gcgacctcat
     1621 cgtcatgtcc tcgcacggcc gcagaggggc gaggcgcctc atcctcggca gccaaactgc
     1681 ggaggtggtt acgaccacta cgatcccggt gcttgtcatc cgctagggct cccaggctgc
     1741 cactgatcgg ttgcgatcag cgcgccccag gccgtgcctt tgggtctgga aggcttcatg
     1801 cgagctcgcc gcgaagcagc cgcggcacct cgcccgacag gccggcagcc tcggcgacga
     1861 acatccgctt cagggtcggc atggtgtcga cgagggctag cccggcctcg cgggccgagc
     1921 gcacgagggc ggcatcgctg gagaacagcc ggttgagcgc gtcgaacgcg gctgcggata
     1981 cgccggaatc gaagcgccgc cattgctcgt aacgctcgag cgcgctgcca tcacccggcg
     2041 tcaatccgac ccgcacagcc tcggccacac actccgtcag cgcagcgata tcccggaacg
     2101 cgagattgag cccctggccg gcgatcggat ggactccgtg ggcagtgtcg cccaccagcg
     2161 cgaatccatg gccgacgtac cgccgggcga ggtgcatctc gagcggccat gaggcgcgcg
     2221 gcccggccag cgtcaggcgg ccatagcggc cggcgaaacg cttgtcgacc tctgccagaa
     2281 aggcctcgtc atcataagcc aggatctccc gggcgcggac cgcatcctcc gaccacgtga
     2341 tgcacgagcg atttcccctc agcggcaaaa tcgcgaacgg cccaccgggt aggaagtgct
     2401 ggatagcgcg tccattgtgg ggcagctcgt gcgcgacctg agttacgatc ccgatctggt
     2461 cgtagctcca gccgatgcat ttgatgccgg ccgcctcgcg caaccgggac ctccgcccgt
     2521 ccgcggcgac gagaaggctc gtctcgatgc tccgcccatc ggcaaggtgg acgcggagga
     2581 acggatcgcc ctccgtgaac cccgttgcct cggcggggcg taggatctgg atggcgggct
     2641 ccgcatcggc agcattctcc agcgccgtgg caagggcctc gttgggcacg atgaacgatg
     2701 ccggctcgcc attttcgagc gtgttgtcgt aggtgagcag gacgggccgc acccccgctt
     2761 ccagcgggct gtcggtgagc tgaatctcca agacctcttg cgcctcgtcc cgcaccttcg
     2821 gccagatgcc gagcacatcc agcatgtgcc gaggcccggc ggagatggca aaggcacgca
     2881 cgtcgcgccc tgtcggaacg acctgccggt cgatgaccgt gatggcgatt tcgggaccaa
     2941 gcgttttcgc cagcgccaac gcgagcgtca gccctgcgta gctgccgccc gatatgacga
     3001 tgtcggacct gagcattccc atgttctggt tcgccttggg gcaacctgtt gcgcaatgct
     3061 agagtttcag gccggtttga tttctacccc agtctctcta cctctagcgc gaggttccca
     3121 tgccctccga aagatccaag ccaacctcgg ccctcgccgc gctcctgtcc atactggacc
     3181 tggagcagct tgaacagaac ctgttccgcg gccagagtcc tcgccagggt tggcagcggg
     3241 tctacggcgg acaagttctg ggacaggcgc tcgtcgccgc agtgcggacc gtttcggagc
     3301 cgcgcacggc ccattccatg cacggctact tcctgctcgg gggcgatccc aatcatccga
     3361 tcatctacga ggtggagcga atccgcgacg gcacgagctt cacgacgcgg agcgtgaagg
     3421 ccatccagca tggccgcgcc atcttcgcga tgagcgtgtc ctttcacaag gaagagccag
     3481 gattcgagca tcagagccag atgcccgatg tcccgccgcc cgaggatctg ccgagcgagc
     3541 accagctcag ggacctgctc gtcgccaagc ttcctgagaa catgcgctcc tattgggagc
     3601 gggagcggcc gatcgaaatg cgccccgtcg acatctcgcg ctacctcaag cgggagaagg
     3661 ctccgccggt ccagtacgtt tggatgcgcg ccaacggcgc gctgccggat gaccccaagc
     3721 tgca
//
LOCUS       DASVDU010000121        22210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_36694, whole genome shotgun sequence.
ACCESSION   DASVDU010000121 DASVDU010000000
VERSION     DASVDU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22210
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36694"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..646
                     /gene="ppa"
                     /locus_tag="VF226_07735"
     CDS             101..646
                     /gene="ppa"
                     /locus_tag="VF226_07735"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX7073915.1"
                     /translation="MRIDAIPIGFDPPNDINVVVEVPSGGEPVKYEMDKASGTLFVDR
                     MLHTAMRYPGNYGFVPHTLSADGDPIDVLIANQRPLIPGCVVNCRPVGVLLMSDEKGG
                     DEKLIAVPSSRVTGRYDKVASYHDLPESLVRQIAHFFEHYKDLEAGKWVRLDRWGDDE
                     EAREFVRQAIARHKEQAPSAP"
     gene            720..1526
                     /gene="dapB"
                     /locus_tag="VF226_07740"
     CDS             720..1526
                     /gene="dapB"
                     /locus_tag="VF226_07740"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX7073916.1"
                     /translation="MSDLKIAVMGAAGRMGRELVRAVHAASGCEVIGGVEREGSPAVG
                     QDIGQLAGLGPLGIPVTDDALELIARADAVLDFTSPKSSVEFANLAANARIIHVLGTT
                     GFSDEDEAAIAAAARHATIIKAGNMSLGVNLLVALTRKVAQALDEDFDIEILEMHHKH
                     KVDAPSGTALMLGEAAAEGRNISHKERSVRVRDGHTGPRKRGDIGYATLRGGNVVGEH
                     TVLFAGEGERIELTHRATDRGIFARGAVRAALWGRGKQPGLYSMMDVLGI"
     gene            1590..2213
                     /locus_tag="VF226_07745"
     CDS             1590..2213
                     /locus_tag="VF226_07745"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEX7073917.1"
                     /translation="MAADNVLVLVRHGESEWNKLNLFTGWRDPDLSEKGVEEARRAGQ
                     LLKAEGYRFDVAFTSALKRAQHTLDIILEQLGQTGLVTYKDQALNERDYGDLSGLNKD
                     DARRRWGAEQVHIWRRSYDVNPPGGESLKDTAARTLPYYEEKIWPEVKAGRNVIVAAH
                     GNSLRSIVMKLDGLSPDEVTKLEIATGVPIVYQLNEDGSVRSKRILG"
     gene            complement(2226..2939)
                     /locus_tag="VF226_07750"
     CDS             complement(2226..2939)
                     /locus_tag="VF226_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7073918.1"
                     /translation="MPRKLKVAALADLHVKEGEVRPYRELFDEICGVADVLVLAGDLT
                     DFGRPEEAHILAEDIRACSLPVLAVLGNHDHDSNQTYEVRRILREAGVKLLEGQTHVI
                     GNVGFVGAKGFGGGFGRRMLTAFGEPAIKQFVAEGMNEATLLENGLRALHTDRIFVIL
                     HYAPVTDTIVGEPLEIFPFLGSSRLAEAIDRYTVSAVVHGHAHKGKYEGRTPAGVPVY
                     NVASHVKKPNGRPYGLIEV"
     gene            complement(2929..3771)
                     /locus_tag="VF226_07755"
     CDS             complement(2929..3771)
                     /locus_tag="VF226_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073919.1"
                     /translation="MAAPVDKGRIFLAERNEATRRQSSSVVATKATPSVTVAGAEAFY
                     RDTLNELLRSGVPFLVAGGFALAAYTGITRETKDLDVFCKAGDYPRVLAHFKRCGYAI
                     SVEDELWIGKVCRGVHYLDVIFAAPSGSAPVTDEWISNARQAEVLGVLAPIIGPTELV
                     WSKCFIQQHDRFDGADVAHLILKAHEDIDWHRLLSRMEAHWEVLLSHLIMFRWIYPSE
                     RDAVPAWLMHELLDRLRVQLEMPPSKRRVCRGGLLARSDYSIDVKEWGFVDFNGKGGP
                     SDAA"
     gene            4014..4589
                     /gene="pyrE"
                     /locus_tag="VF226_07760"
     CDS             4014..4589
                     /gene="pyrE"
                     /locus_tag="VF226_07760"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX7073920.1"
                     /translation="MPDLDRSPAADRARLIEIVKARSFESGREVKLASGRTSTFYFNM
                     KPTMLDAEGGLLVANLILDQLHDVPARWIGGLEMGAVPIAAAVAAVSPLRHRPVSAFF
                     VRKQAKDHGTRSLIEGLAPGETLNGADVVVVEDVTTTGGSAIKAAEAVRAEGGKVVRV
                     ITIVDRLEGAADAFRAAGLEFVPLLTIADFR"
     gene            complement(4691..4987)
                     /locus_tag="VF226_07765"
     CDS             complement(4691..4987)
                     /locus_tag="VF226_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 domain-containing protein"
                     /protein_id="HEX7073921.1"
                     /translation="MNRTNRTRSPSKRAKRATMTPVMSQLELARLGGGEIAYIKVLSS
                     DEAQRLYPAIEGLPGGIDLYALHAADGTPIALTDSRQAAIGHARGDKLEVASVH"
     gene            complement(5102..5539)
                     /locus_tag="VF226_07770"
     CDS             complement(5102..5539)
                     /locus_tag="VF226_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX7073922.1"
                     /translation="MSRMSLLSSPMLLGFEEIERLIDRAGKGSGDGYPPYNIERIVKD
                     GERGEILRITLAVAGFTRDQLEITVEDNQLTIRGKQQEEKSRDFLYRGIAARQFQRTF
                     VLADGIEIRSADLSNGLLSIDLVRHEPERLVRRIEIGERKSGA"
     gene            5809..6462
                     /locus_tag="VF226_07775"
     CDS             5809..6462
                     /locus_tag="VF226_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073923.1"
                     /translation="MPVRLALRQILAVGIVVLFAAPAAAQDQQVPPVQEETELTPEEK
                     AERESRKACKVAICDAFYNRDPNGSDISCHVVKTWRKEHLQKIVSKAKVSWPWGRVQC
                     VADIKLERDMLIKAMTEPKHEAVLQKHQVACKIAREEGDAELKFDFSPKVTFEGGKAT
                     KAVLNWGKIEAPTLVKGAMWTATATDNTFNVLDSMVVEDINDFISKKCMEVKDEWAK"
     gene            complement(6487..8319)
                     /locus_tag="VF226_07780"
     CDS             complement(6487..8319)
                     /locus_tag="VF226_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7073924.1"
                     /translation="MAPPLVALRDTHLTYGGTPLLTGAELSVEPGERVCLVGRNGSGK
                     STLLRIAAGLTEPDRGERFAQPGALVRYLPQEVDFSGYETALEFVEAGLTPTQDRYQA
                     RYLLEVLGLTGAEPVATLSGGEARKVALARVLAPEPDVLLLDEPTNHLDLPAIEWLEA
                     ELLRSRAALVIISHDRRFLETLSRATVWLDRGVTRRLDQGFAAFEAWRDAMLEQEEIE
                     RRKLDRKIAREESWLHGGVTARRKRNVRRVAELARMRQERREARRVQGDVRFSVGQSG
                     PSGRLVIEAVGIGKSFGDRTVVRDFSTRIARGDRVGIVGPNGAGKTTLLKMLTGRLAP
                     DTGEIRLGTAIDMVTLDQRRDELAPTMTVMEALTGGRGDKVIINGQPRHVSSYMKDFL
                     FQPEQALSPLSVLSGGERARLMLARALAKSSNLLVLDEPTNDLDLETLDLLQELLADY
                     TGTVLLVSHDRDFLDRVVTSVIAAEGDGRWVEYAGGYSDMLAQRAGADLPPSGTAGSA
                     AAEQKPKATVEAGTGTAPQKKRKLSFNEKHALEMLPGTIDALQREIADLQAVLSDPEL
                     YSRDPQAFEAATARLAKAEAELTQAEERWLELELLREELEGAGS"
     gene            8495..8887
                     /gene="apaG"
                     /locus_tag="VF226_07785"
     CDS             8495..8887
                     /gene="apaG"
                     /locus_tag="VF226_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX7073925.1"
                     /translation="MYEAVTRGIRVRVTPAYTEEQSNPEENYFFWVYTIVIENIGDAP
                     VQLRSRIWHITDAHGHTEEVRGPGVVGLMPIIPPGESFSYTSGCPLRTPQGIMVGSYQ
                     MVDAEGSTFDVVIPAFSLDSPFAKRNLN"
     gene            complement(8911..9882)
                     /locus_tag="VF226_07790"
     CDS             complement(8911..9882)
                     /locus_tag="VF226_07790"
                     /inference="COORDINATES: protein motif:HMM:NF002386.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEX7073926.1"
                     /translation="MAERQTLAAAVEPRRSDDLIMPFRTNRSGVFGRVVRLGPVIDSI
                     LNRHDYPEPVSRVLGEAVALTTLLGSSLKFDSAFSGRLILQTKTDGPINLLVVDFEEP
                     GRVRAYASFNEDKVEALGRSAREEPGLLLGTGHLAMTIDPGGHLDRYQGIVALDGSGL
                     SEAAHTYFRQSEQIPTFLRLAVARHFAGGRWQWRAGGLLVQYVPPVEEGATSDDENEE
                     VLLGEDDERWTSTRVLAETVEDHELLDPTLTPERLLYRLFADEGVRAFPAVGLEERCR
                     CSRDRVSKLLGSFGAEELQDMREADGAITVTCEFCNTKYRFETEEIV"
     gene            complement(9883..10806)
                     /gene="argF"
                     /locus_tag="VF226_07795"
     CDS             complement(9883..10806)
                     /gene="argF"
                     /locus_tag="VF226_07795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7073927.1"
                     /translation="MAPRHFIDIGDIDAATLRRIVDVAHEMKRAGKVVPARLRPEGIE
                     HSVLVMIFEKPSTRTRVSFDVAMRQLGGQTIALNHTDLQLGRGESIADTARVLSRYID
                     VIMIRANTHESVVELARHASVPVINGLTDKSHPCQIIADIMTFEERKGPIAGQTVAWV
                     GDGNNVAASWVHAAKRFGFKLRIASPVQLKPERPVLDWVAAEHADVVITDDPEEAVAG
                     ADCVVTDTWVSMGHTDAPRRKQMLGPYAVDSDLMRKAASGAIFMHCLPAYRGNEVSAE
                     VIDGPQSVVFDEAENRLHAQKAILAWCLGKD"
     gene            complement(10839..12005)
                     /locus_tag="VF226_07800"
     CDS             complement(10839..12005)
                     /locus_tag="VF226_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7073928.1"
                     /translation="MGTYARQDVVFERGEGCWLTSTTGERYLDFGSGIAVNLLGHAHP
                     KLVTALTEQAQKLWHTSNLYRIEGQERLAERLCAATFADRVFFCNSGAEACEGAIKLA
                     RRYHHVSGNPERYRTITFQGAFHGRTLATIAAAGNEKYLEGFGPRVPGFDVLPAGDMA
                     AVEAAIGPETAAIMIEPVQGEGGIRVIPADDLRRLRELCDAHGLLLVLDEVQCGMGRT
                     GKLFAHEWAGITPDVMAIAKGLGGGFPVGAFLATAEAAKGMVPGTHGSTFGGNPLAMA
                     VGNAVMDVVLEPGFLETVQQKAIRFKQELARIKDEFPGVVEEVRGMGLLTGLRLKPPV
                     ADVVAACAAEKLLAVSAGDNVLRLLPPLNITEAEMAEGLSRLSKALRRISTTQA"
     gene            12610..13173
                     /locus_tag="VF226_07805"
     CDS             12610..13173
                     /locus_tag="VF226_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEX7073929.1"
                     /translation="MGWTDERVEQLKRLWAEGLSASQIAGRLGGVTRNAVIGKVHRLG
                     LSGRATTSRMKSHRARPRTSPGSVTKRPAAKPRFGTIGNPALRALYQPDAETYVPPAE
                     ELVIPPEERKTLQMLTETSCRWPIGDPQTPEFHFCNRNKVPGLPYCEIHARRAFQPPQ
                     PRRRDREVGEPVVPAIPAQAAKDPVDA"
     gene            13323..13904
                     /locus_tag="VF226_07810"
     CDS             13323..13904
                     /locus_tag="VF226_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEX7073930.1"
                     /translation="MNAQPNATFLSTVWPESRDSFLRLGAVVVLGTLLLTASAKIQVP
                     FWPVPMTMQTFMVLFLGAALGPRLGALTVLLYLTQGAAGLPVFAGTPEKGLGIAYMAG
                     PTGGYLLGFTAGAYVVGWLAERGWDRSVLRLFAAMALGHVVIFAFGVAWLTQLVGLEK
                     AWLLGVTPFYAATLFKTALGACLMPALWQLAKR"
     gene            complement(13978..14349)
                     /locus_tag="VF226_07815"
     CDS             complement(13978..14349)
                     /locus_tag="VF226_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073931.1"
                     /translation="MLNTAYSGLAAAGKAIEPWLRAAARGNIEAFTLMNRRAQAYMEL
                     PMRLSQCRSPQDLANEQMRFWQTMARQYTESSQRVLGAWFEMVQKSAGKAKSSSAEHE
                     RDYITFPEPEETGSSAERRAA"
     gene            complement(14537..16120)
                     /locus_tag="VF226_07820"
     CDS             complement(14537..16120)
                     /locus_tag="VF226_07820"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEX7073932.1"
                     /translation="MKAFAALLDRLVYSPGRNTKLQLMQEYFRTTPDPDRGWALAALT
                     DGLPFSFPVRRTIVELMDKRLDAELFRLSRDYVGDTAETVALLWPDTEAQGDPPRLSE
                     VVSRLGLIDAQELGDVIASWLDRLDATGRWALLKLLTGALRVGVSARLAKTAVAGMAG
                     HSVDDVEEVWHALSPPYQPLFDWLEGHAERPDIAGAPVFRPLMLAHALEDSELADLDI
                     GEFSVEWKWDGIRVQIVAGRGEVRLYSRTGDEIGASFPELISMFRFDAVIDGELLVLR
                     DGKVAPFNDLQQRLNRKSVTRAMLERYPAHVRLYDALLIDGEDLRPLPFIERRRRLER
                     WHAEVRPPRTDVSELIAVADKEELHRIWRETRGAGIEGLMLKRRASPYLAGRPRGHWY
                     KWKRAPITLDCVLMYAQRGSGRRSSYYSDYTFGAWKELPDGSLELVPVGKAYSGYTDA
                     ELIVLDRWIRNNTVERFGPVRAVRPALVLEVAFDAVQRSTRHRSGVAMRFPRIHRIRW
                     DKPAEEAERLDTLVGLIEG"
     gene            complement(16197..17207)
                     /locus_tag="VF226_07825"
     CDS             complement(16197..17207)
                     /locus_tag="VF226_07825"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEX7073933.1"
                     /translation="MIAFEDWLQLTEAGLYCAPGGFHIDPSRPVDRAVITHGHSDHAR
                     GGHGAVLATPETVKVMQARLGKDSAGRFQPQPLNDPLRHGDVTIRLHPAGHILGSAQI
                     ELTYRGQRAVVSGDYKRTPDPTCALFELVPCDLFVTEATFGLPVFRHEPDWREIGRLL
                     ASVQAFPERTHLIGCYGLGKCQRLLALLRRAGYDRPIYLHGALKSMTDLYIACGQSFG
                     ELVLVSEAGRGLAGEIVLCPPSALKDRWSRRLADPVTAFASGWMRVRGRARQRGVELP
                     LVISDHADWPQLIETIAETGAEEVWVTHGREEALVYQLGRMGKRARALALLGREDEEA
                     ED"
     gene            17443..18177
                     /gene="bcsS"
                     /locus_tag="VF226_07830"
     CDS             17443..18177
                     /gene="bcsS"
                     /locus_tag="VF226_07830"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HEX7073934.1"
                     /translation="MGRLIGSSKVAWRGGTIGLGAAFVLLASPVAAQQAKPTQEVWGG
                     ADVTTNSWSVYSGLTVALFSSLYDNGLRLRAVGGYGFYSYPKYDVTIRGYVGFGDLLL
                     GYQHQFGPLTLKAFMGLSTESHNLAPPDPDSSVVGADLGAKVALESWWEIGPKTWSSL
                     DISWSTVHGGTYAARARAGYRLRPQLSVGIEAAADGDAEYDGGRGGGFLRYTWAAGEI
                     SASAGASIDRSGESGGYGAMNALFRY"
     gene            18325..19158
                     /locus_tag="VF226_07835"
     CDS             18325..19158
                     /locus_tag="VF226_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7073935.1"
                     /translation="MKSILVPIEKHDLVTSMLDCASRLAGRFGGIVEGVALRLPQISV
                     VGPDPVVTVTFPRAEQEDLEIISTARRIFDAYFASETAEGLPESQRPRHRWRAVDPVD
                     DAALASLARVYDLTVVGRPQAGTAGPRMTTLESVLFESGRPLLIAPPQAPRSLGDRIV
                     ISWNCSSESARTVAYAMPLLLKAREVTVLTVEGAVSPGPSGKELADCLAVHGIEAREL
                     TALPGGRKPGEAILEEAQRLGADLLLKGAYTQSRLRQMIFGGATSHILSNTELPVFMA
                     H"
     gene            complement(19347..20330)
                     /locus_tag="VF226_07840"
     CDS             complement(19347..20330)
                     /locus_tag="VF226_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEX7073936.1"
                     /translation="MSANLAFGGLVTVFGGSGFVGRHAVQQLAKRGWRIRAAVRRPDL
                     AGHLQPMGRLGQIHAVQANVRFPESIEHAVKDAVAVVNAVGILIPSGRQSFHAVHVEG
                     SRAVAKAARQAGAQRFVHISAIGADPASRSAYARTKAEGEKAVLDEFPEAVIIRPSVV
                     FGPEDDFFNRFGSLARFSPFLPLIGGGRTRFQPVYAGDLGRAIANAVNGTGTPGTVYE
                     AGGPEIFTFRELLDRVKEWTDLHRPYLPIPFWLAQLVALLTWPLPNSIRPITLDQIRL
                     LRMDNVVSAAAQEEGRTLQGLGVTQLHTIDLIVPDYLEPFRPYGQFARYRG"
     gene            20456..20540
                     /locus_tag="VF226_07845"
     tRNA            20456..20540
                     /locus_tag="VF226_07845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20490..20492,aa:Leu,seq:cag)
     gene            21800..>22210
                     /locus_tag="VF226_07850"
     CDS             21800..>22210
                     /locus_tag="VF226_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073937.1"
                     /translation="MAEAVRRAADGDVIRLEPGRYSGPVIVEGKRIVIEGPSGGQAVI
                     VPGRSNVLVGAQNGGQVELSGLKFETSAETPMALFAKGGGISCADCTIESAQNQPVYV
                     ESGTLALNSSKVSGAGNHAIAARDSAITIEGTQVE"
BASE COUNT         3992 a         7165 c         7065 g         3988 t
ORIGIN      
        1 aagcgttcag cctacacgcc acgctctatt ccttacgggc agcgcacacg ggcgctgccg
       61 ggcagccgct gacctggtga gcgaggaaag gatcactccg atgcgcatcg acgccatacc
      121 gatcggcttc gacccgccca acgacatcaa cgtcgtcgtc gaggtcccca gtggagggga
      181 gcccgtgaaa tatgaaatgg acaaggcatc cggcacgttg ttcgtcgaca ggatgctgca
      241 tacggccatg cgctatccgg gcaactacgg gttcgttccg catacgcttt cggccgacgg
      301 cgatcccatc gacgtcctca tcgcaaacca gcgccctctc attcccggtt gcgtcgtcaa
      361 ttgccgtccc gtaggggtgc tcctgatgag cgatgagaaa ggcggtgacg agaagctcat
      421 cgccgtcccg tcgagccgcg tgacgggtcg gtacgacaag gtggcgagct accatgatct
      481 acccgaaagc ctcgtccggc agatcgcgca ttttttcgag cactacaagg atctcgaggc
      541 gggcaagtgg gtgcggctcg atcgctgggg cgacgacgag gaagcacgcg agttcgttcg
      601 ccaggccatc gcgcgtcaca aggagcaggc gccatctgcg ccctgatccg ctccgccgtt
      661 ctaatccgcg gccggctctg ctataggccc ggcaagttcc ggtgcgagag gagacgaaga
      721 tgagcgattt gaagatcgca gtcatgggcg ccgccggccg aatggggcgc gagctcgtgc
      781 gggccgttca cgccgcgtcg ggatgcgagg tcatcggcgg cgtcgagcgc gagggctcgc
      841 ctgccgtggg ccaggacatc ggccagctcg ccgggctcgg cccgctcggg atccccgtca
      901 cagacgatgc gctggagctg atcgcccgtg ccgacgctgt gctcgacttc acctctccca
      961 agtcgtcggt cgaattcgcc aatctcgccg cgaacgcgcg catcatccac gtcctcggca
     1021 cgaccggctt ttccgacgag gacgaagcgg cgatcgccgc agccgctcgc cacgccacga
     1081 tcatcaaggc cgggaacatg agcctgggcg taaacctcct ggttgccctc acgcgcaagg
     1141 tggcgcaggc cctcgacgag gacttcgaca tcgaaatcct ggagatgcat cacaagcata
     1201 aggtggatgc cccctccggc acggcactga tgctgggaga ggctgctgcc gaggggcgaa
     1261 acatctcgca caaggagcgg agcgtgcggg tgcgcgatgg ccatacaggc ccgcgcaagc
     1321 gcggagacat cggctacgcg acactgcgcg gcgggaacgt cgtcggcgag cacacggttc
     1381 tgtttgccgg ggaaggtgaa cgcatcgagc tcacgcaccg cgcaaccgat cgcggcatct
     1441 tcgcccgcgg ggcggtgcgg gccgccttgt ggggacgagg caagcagccg gggctctatt
     1501 ctatgatgga tgtgctcggc atctgagccg ccgcttgcgg actagcttaa ccgaaagtca
     1561 gaactaaaac taaccagaca ggattggaaa tggcggctga caacgtgctc gtgctggttc
     1621 gtcacggcga aagcgaatgg aacaagctca atctgttcac cggctggcgt gacccggacc
     1681 tttccgaaaa gggcgtcgag gaagcacgca gggcagggca gctgctcaag gccgagggct
     1741 acaggttcga cgtcgcgttc accagcgcct tgaaacgcgc acagcatacg ctcgatatca
     1801 tcctggagca gctgggccag accgggctcg tcacatacaa ggatcaggcg ctgaacgagc
     1861 gcgactatgg tgatctctcc gggctcaaca aggacgacgc gcgccgccgc tggggagccg
     1921 agcaagtgca catctggcgg cgcagctacg acgtgaaccc gccgggcggc gaaagcctga
     1981 aggacaccgc cgccaggacg ctcccgtatt acgaggagaa gatttggccg gaggtgaagg
     2041 ctgggcgcaa cgtcatcgtc gcggcgcacg gcaattcgct gcgatccatc gtgatgaaac
     2101 tcgatggcct ctcgcccgac gaagtcacga agctcgaaat cgccaccgga gtgccgatcg
     2161 tctatcaatt gaacgaggat ggctcggtga gatccaagcg cattctgggc tgagcttccc
     2221 cagcctcaaa cctcgatcag tccgtacggc cggccgttgg gcttcttgac gtgcgaggcg
     2281 acgttgtaga cgggcacgcc ggctggcgtg cggccctcgt atttgccctt gtgagcgtgc
     2341 ccgtgtacga ccgcgctcac tgtataccga tcgatggctt cggcgaggcg tgaggagccg
     2401 aggaacggaa agatttcgag cggctcaccc acgatcgtgt ccgtgaccgg cgcgtaatgg
     2461 agaatgacga aaatccggtc cgtatgcagc gcccgcaatc cgttctccag cagcgttgcc
     2521 tcgttcatgc cctctgctac gaactgcttg atggccggct ctccaaacgc cgtcagcatc
     2581 cgccggccaa agccaccgcc aaatcctttc gccccgacaa agccgacatt cccaatgacg
     2641 tgtgtctggc cttcgagcag cttgaccccg gcttcgcgca agatccgtct cacctcgtag
     2701 gtttggttcg agtcgtggtc gtgattgccc aacactgcca gaaccgggag cgagcaggcc
     2761 ctgatatcct cggccaggat gtgcgcttcc tccggcctgc caaagtccgt caaatcgccc
     2821 gccaggacga gaacgtccgc aacaccgcag atctcatcga acagctcgcg atagggccgc
     2881 acctctcctt ccttcacgtg caggtcggcg agggcagcga ccttcagctt acgcggcatc
     2941 gctcgggcca cctttcccgt tgaaatccac gaagccccac tccttcacat cgatgctgta
     3001 atcggaccgt gccagcagac cgccccggca gacccgcctt ttcgaaggcg gcatctcgag
     3061 ctggactcgc agacgatcca acagctcgtg catgagccag gccggcaccg cgtcccgctc
     3121 cgatgggtag atccagcgga acatgatgag atgcgataat aggacctccc agtgtgcctc
     3181 catccgcgaa agcaggcgat gccagtcgat gtcctcgtgc gccttaagga tcagatgggc
     3241 gacgtcggca ccgtcgaaac ggtcatgttg ctgaatgaag cacttcgacc agacgagctc
     3301 tgtcgggccg atgatcggcg cgagcacgcc caggacctcc gcctgacgtg cgttgctgat
     3361 ccactcgtcg gtgacgggcg cgctgccgct gggtgcggcg aaaatcacgt cgaggtaatg
     3421 aacccctcta caaactttgc cgatccagag ctcgtcctcg accgagatgg cgtaaccgca
     3481 tcgcttgaag tgcgcgagca cgcgaggata gtcgcccgcc ttgcagaaga cgtcgagatc
     3541 cttggtctcg cgcgtaatcc ccgtataggc cgcgagcgca aagccgccag cgaccaggaa
     3601 cggcacgcca ctgcgcagga gctcgttcag ggtgtcacga taaaacgcct ccgcgccggc
     3661 cacggtgaca ctgggcgttg ctttcgttgc gacaaccgag gagctttgcc gtctggtagc
     3721 ctcgttccgt tccgccaaga agattcgacc cttgtcgaca ggggcagcca ccgtcgctcc
     3781 atcgcggcga gcgcgcccag cccatcgcct gaaatcgtgc ctgagcaacg gcagtccgct
     3841 caccaggttc cgcatcagga gagcagtggc tttgaccgcc ggctcgggcg tgctgagggg
     3901 tgtcgcgaaa aagatgtgtc gatgccgctg cggcggagcg actgtacttc ttgacagcgc
     3961 cgcccctgca gccacaacgc acgtcagccg ccattccagg ggaggagcct gacatgcctg
     4021 acctcgaccg ctcgcccgcc gcagaccgcg cgcgcctcat cgagatcgtc aaagcgcgct
     4081 cgttcgagag cgggagagag gtgaagctcg catccgggcg cacgtcgaca ttctacttca
     4141 acatgaagcc gacaatgctc gatgccgagg gcgggctgct cgtcgccaac ctcattctcg
     4201 accagctcca cgacgttccc gcccggtgga ttggcgggct cgagatgggc gcggtgccca
     4261 ttgccgcggc cgtggcggcg gtcagcccgc tccggcaccg gcccgtttcg gcgttcttcg
     4321 tgcgcaagca ggccaaagat cacggcaccc ggagcctgat agagggcctt gcgcccggcg
     4381 agacgctgaa cggtgccgac gtggtggtgg tggaggacgt gacgacgacc ggcgggtccg
     4441 caatcaaggc agcggaagcg gtgcgggccg aaggcggcaa ggtcgtgcgg gtgatcacga
     4501 tcgtcgatcg gctggaagga gccgctgacg cattccgggc ggcgggcctc gagttcgtgc
     4561 cattgctgac gatcgcggac ttccgctgat cggaacggac atcactctcg aacaccgatc
     4621 cgcaaaaagc aaaaaagcgc cccgctcgca agcgaggggg cgctttgatt tgcctcacaa
     4681 cgacgctgct tcagtggacg ctggccacct ccagcttgtc tccgcgggca tgtccgatgg
     4741 cagcctggcg gctgtcggtc agggcgatgg gcgtgccgtc ggccgcgtgg agcgcgtaga
     4801 gatcgatgcc gccgggaagc ccttcgatcg ccgggtaaag ccgctgtgcc tcgtcggagg
     4861 acagcacctt gatgtaggcg atctcaccac cgccgagccg cgccagctcc agctggctca
     4921 tgaccggcgt catcgttgcg cgcttggcgc gcttgctggg tgatctcgtg cgatttgtcc
     4981 tgttcatgac gctactcctg caggtgcggg aaccggcacg tcatgccttg cagcctgcac
     5041 cctcggccgg ctgccgcagc accatgctcc cgtgattctc tgtctttggc cagcgcggtg
     5101 ctcatgcacc cgatttgcgc tcaccgatct cgattctccg gaccagccgc tccggctcgt
     5161 gcctgacgag atcgatggac agaagaccgt tgctcaggtc tgcactgcgg atctcgatgc
     5221 catccgccaa tacgaaggtg cgttggaatt gccgagccgc gatcccccga tagagaaagt
     5281 cccggctctt ctcctcctgc tgcttgccgc ggatcgtaag ctgattgtcc tcgacggtta
     5341 tctccagctg atctcgtgtg aagcctgcca ccgccagcgt gatgcgcaaa atctcgcctc
     5401 gctcgccatc tttcacgatg cgttcgatgt tgtagggcgg atagccgtcg ccgcttccct
     5461 tcccggcgcg atcgatcagc cgctcgatct cctcgaagcc gagcagcatg ggactggaca
     5521 aaagagacat gcgtgacatt ccaaagccct ttccaagcga ccttgttgac gtgttacgcg
     5581 ggtcccgaga gggcccccac gctgccggcc ccgacaaatc cgggcaccgt tccgcacgcg
     5641 aaactcagat atggtgccgc gacaagctag ttcaagcaag gactagatgt ccgccctgct
     5701 tgcgcgatct gctcaggccg atgcaaaagc tgcgcggtct ggcgtcatat tcctgcgaca
     5761 gtcgaacacc attaagcata cgtctcaacc gcgacacggg gggtgtccat gccggttcgt
     5821 cttgcgcttc gtcagatcct tgccgtgggc atcgttgtgc tttttgcagc gccggccgcc
     5881 gcgcaggacc agcaggtgcc tcccgtacag gaggagacag agctcacgcc cgaggagaag
     5941 gccgagcgcg agagccgcaa ggcctgcaag gtggcgatct gcgatgcatt ctataaccgc
     6001 gacccgaacg gcagcgacat ctcatgccac gtggtgaaga cctggcgcaa ggagcatctt
     6061 cagaagatcg tctccaaggc gaaggtgtcg tggccctggg gccgggtaca gtgcgtggcg
     6121 gacatcaagc tcgagcgcga catgctcatc aaggcgatga ccgagccgaa acacgaagcc
     6181 gtgctccaga agcaccaggt cgcctgcaag atcgcgcgtg aagagggcga cgccgagctg
     6241 aagttcgact tcagcccgaa ggtcaccttc gaaggcggca aggcgaccaa ggccgtgctc
     6301 aactggggca agatcgaggc accgacgctc gtcaagggcg cgatgtggac ggcgaccgcc
     6361 accgacaaca ccttcaacgt cctcgacagc atggtcgtgg aggacatcaa cgacttcatc
     6421 tcgaagaagt gcatggaggt gaaggacgag tgggccaaat gacgcccgac ccaaacggct
     6481 gctgcttcag cttccggcgc cttccagctc ctcccgcaac agctccagct cgagccagcg
     6541 ttcctcggct tgggtgagct cggcttcggc cttggccaac cgggccgtgg cggcctcgaa
     6601 ggcctgcggg tcgcgactgt agagttcggg gtccgacagc accgcctgga ggtctgcgat
     6661 ctcgcgctgc agggcgtcga tcgtgcccgg cagcatctca agagcgtgct tctcattgaa
     6721 cgagagcttg cgcttcttct gcggcgcggt accagtgccg gcctccactg ttgccttcgg
     6781 tttttgctcg gccgcagcag aaccggcagt tccggacggc ggcaggtcgg cgccggcgcg
     6841 ttgcgccagc atgtcggaat agccgccggc atactccacc caccgcccgt cgccttccgc
     6901 agcgatgacc gacgtgacga cgcgatctag gaagtcgcgg tcgtggctga cgagcagcac
     6961 cgtgccggtg tagtcggcca gcagctcctg cagcagatcc agcgtctcga gatcgagatc
     7021 gttcgtcggc tcgtcgagga cgagaaggtt ggacgacttg gccagtgcgc gcgccaacat
     7081 caaccgcgcc cgctcgccgc ctgaaagcac tgaaagtggg ctcagcgcct gctcgggctg
     7141 gaacaggaag tccttcatgt agctcgagac atgccgcggc tggccattga tgatgacctt
     7201 gtcgccgcgg ccgccggtca gggcttccat gacggtcatc gtgggtgcca gctcgtcccg
     7261 gcgctggtcg agcgtcacca tgtctattgc agtgccgagg cgtatttccc cggtatcggg
     7321 cgccagccgg cccgtcagca tcttcagcag cgtggtcttc ccagcaccgt tcgggccgac
     7381 gatgccgacg cggtcgccgc gcgcgatgcg ggtcgagaag tcgcgcacga cggtcctgtc
     7441 cccgaagctc ttgccgatgc cgacggcctc gatcacgaga cgaccggatg ggccgctttg
     7501 gcccacgctg aagcggacat cgccctgcac gcggcgggcc tcacgccgct cttggcgcat
     7561 gcgggccagc tcagcgaccc gccggacatt ccgctttcgg cgggcggtga cgccgccgtg
     7621 cagccagctt tcctcgcgtg cgatcttgcg gtcgagcttg cgccgctcga tctcctcctg
     7681 ctcgagcatc gcgtcacgcc aggcctcgaa ggcggcgaag ccctggtcga ggcggcgggt
     7741 cacgccgcgg tcgagccaca ccgttgcccg cgacaaagtt tcgaggaaac ggcggtcgtg
     7801 actgatgatg accagcgccg cgcgagatcg cagaagctcc gcctcgagcc actcgatcgc
     7861 cggcaggtcg aggtggttgg tgggctcatc gagcagcaaa acatcaggct cgggcgccag
     7921 gacgcgggcc aaggcaacct tccgcgcctc gccacccgag agggtcgcaa cgggctcggc
     7981 gccggtcagc ccgagcactt ccaagaggta gcgcgcctga tagcgatcct gcgtcggcgt
     8041 gaggccggct tcgacgaatt ccagcgccgt ctcgtagcct gagaaatcga cctcctgcgg
     8101 caggtagcgc accaaggcac ccggctgggc gaaccgctcg ccccggtccg gctcggtcag
     8161 cccggcagcg atccgcagca gcgtcgactt gccggacccg ttccgtccca cgaggcagac
     8221 gcgctcgccc ggctccacgg acagctcggc tcccgtcagc agcggcgtgc cgccgtatgt
     8281 cagatgagta tcgcgtagcg ccaccagagg aggtgccatc aagtccctca cttcgatttt
     8341 gtgatgttta gctcacgcct gcggaagcga tcaaagccgc cccccggtca ttcatccgca
     8401 tttgaggcgc ccgctgcatc atattgaccg agttggtccg agttctagta tggctgtact
     8461 ggccacactg cccgatcgtg agagtgccct cacaatgtac gaagccgtta cccgcggcat
     8521 ccgtgtccgt gtcactccgg cctacacaga ggagcagtcg aatccagagg agaattattt
     8581 cttctgggtg tacacgatcg tgatcgagaa catcggcgac gcgcccgtgc agctccgctc
     8641 tcgcatctgg cacatcacgg acgctcatgg ccataccgag gaggtgcggg ggccgggcgt
     8701 cgtggggctg atgcccatca ttccgcccgg tgagtccttc tcgtacacgt caggatgtcc
     8761 gctgcgcacg ccgcagggca tcatggtcgg cagctatcag atggtcgatg cggaaggcag
     8821 caccttcgat gtcgtaattc ccgccttctc gctcgacagc cccttcgcca agcgcaacct
     8881 caactgagga cgacgagcct ccccgccacc tcagacgatc tcctcggtct cgaagcggta
     8941 cttggtgttg cagaactcgc acgtgacggt gatcgcacca tcggcctcgc gcatgtcctg
     9001 caattcctcg gcgccaaagc ttccgagcag cttgctgacc cgatcgcggg agcagcggca
     9061 gcgctcctcg agaccgacag cgggaaacgc acgcacgccc tcgtcggcga agagccggta
     9121 gagcagtcgc tctggcgtca gggtcggatc gagcagctcg tgatcctcga cggtctcggc
     9181 cagaacgcgc gtactggtcc agcgctcgtc gtcctcgccc agcagcacct cctcgttctc
     9241 gtcgtcggag gtcgcgcctt cctccacggg cggaacgtac tggaccagca gcccgccggc
     9301 gcgccactgc catctgccgc ccgcgaaatg gcgggcgacg gccaggcgca gaaacgtcgg
     9361 gatctgctca gactgccgga aataggtgtg cgcggcttcg ctcagcccgg agccgtcgag
     9421 ggccacgatg ccttggtatc ggtcgagatg gccacccggg tcgatggtca tcgcgaggtg
     9481 ccccgtgccg agcagcagcc ctggttcctc gcgcgcggag cgccccagag cctcgacctt
     9541 gtcctcgttg aagctcgcat aggcgcgtac gcggcccggc tcctcgaagt cgacgaccag
     9601 cagattgatc ggcccgtcgg tcttcgtctg gagaatgagc cgcccgctga aggcgctgtc
     9661 gaacttgagc gatgagccga gcagcgtggt gagcgctacg gcctcaccca gcacccggct
     9721 gacgggctcg ggataatcgt gccggttcag gatgctgtcg ataacggggc caaggcgcac
     9781 gactcggccg aaaacgccgg accgattggt ccggaatggc atgatcaggt catcggacct
     9841 tcgcggctcg acagccgcgg cgagagtttg gcgttctgcc attcaatcct tcccaaggca
     9901 ccatgcgagg atcgcttttt gagcatggag ccggttttct gcttcatcga acacgacgga
     9961 ctgcggcccg tcgatcactt ccgcgctgac ctcgttgccg cggtaggcgg gaaggcaatg
    10021 catgaatatg gccccgctcg ccgccttacg catgaggtcg ctgtcgacgg catatggtcc
    10081 cagcatctgc ttgcgtcgag gggcgtcggt gtggcccatc gagacccagg tgtcggtgac
    10141 gacacaatcg gcccccgcaa ccgcctcctc aggatcatcg gtgatcacaa cgtcggcatg
    10201 ctcggcagca acccagtcga gcaccggccg ttcgggcttg agctggacgg gcgaggcgat
    10261 ccgcagcttg aagccgaacc gcttggcggc gtgtacccag gacgccgcga cgttgttgcc
    10321 gtcaccgacc caggcaacgg tctgaccggc gatcggcccc ttgcgctcct cgaacgtcat
    10381 gatgtccgcg atgatctggc agggatggct cttgtcggtc aggccgttga tcaccggcac
    10441 ggaggcgtgg cgcgcaagct ccaccacact ctcgtgcgtg ttggcgcgga tcatgatgac
    10501 gtcgatgtag cgggacagca cgcgcgccgt atcggcgatc gactctccgc gcccgagctg
    10561 caggtcggta tgattaagtg caatggtctg gccgccgagc tgccgcatcg cgacatcgaa
    10621 cgacacgcgc gtgcgggtcg agggtttctc gaagatcatt accagcacgg aatgctcgat
    10681 gccttcgggg cggagcctcg ccggcaccac tttgccggcg cgcttcatct cgtgcgcgac
    10741 atccacgatg cgccgcaggg ttgcggcatc gatgtcacca atatctatga aatggcgtgg
    10801 ggccatgtca gcacctcgcg gatcggcggc ctgcgccgtc aggcttgggt ggtggaaatg
    10861 cggcggagcg ctttggacaa gcgcgataga ccttctgcca tctctgcttc agtgatgttg
    10921 agcggcggca gcaatcgcag cacgttgtcc ccggctgaga cggccagcag cttctcggcc
    10981 gcgcaggctg cgaccacgtc cgccacggga ggtttcagcc ggagcccggt gagcagaccc
    11041 atgccgcgga cttcttccac cacgccgggg aactcgtcct tgatccgggc cagctcctgc
    11101 ttgaaccgga tcgccttctg ctgaaccgtt tccaggaaac ctggctcgag cacgacgtcc
    11161 atgacggcgt tgccgaccgc catggccagc gggttgccgc cgaaggtcga cccgtgcgtg
    11221 cccggcacca tgcccttcgc cgcttcggcc gtcgcgagga atgcgccgac ggggaatcca
    11281 cccccgagac ccttggcgat cgccatcacg tcgggtgtga tgcccgccca ttcgtgcgcg
    11341 aacagcttgc cggtacggcc catgccgcat tgtacctcat cgaggacgag gagcaggcca
    11401 tgggcgtcgc aaagctccct cagacggcgt agatcatcgg ccgggatgac gcggatgccg
    11461 ccctcgccct gcacgggctc gatcatgatg gcggccgtct ccggcccgat cgccgcctcg
    11521 acggcggcca tgtctcctgc cggcagcacg tcgaagcccg gcacccgcgg accaaagcct
    11581 tcgagatact tctcgttgcc ggcagcggcg atcgtcgcca gcgtccgtcc gtggaaggcg
    11641 ccctggaatg tgatggtccg gtagcgctcg gggttaccgg acacgtgatg atagcggcgg
    11701 gcaagcttga tcgcgccctc gcaggcttcc gcacccgagt tgcagaagaa gacgcggtcg
    11761 gcaaacgtcg ccgcgcaaag ccgctcggca agccgttcct ggccctcgat ccggtagagg
    11821 ttcgacgtat gccagagctt ctgcgcctgc tccgtcagcg cagtgacgag cttcggatgg
    11881 gcatggccga gcaggttgac cgcaatcccg ctgccgaagt ccagataacg ctccccggtc
    11941 gtggaggtca gccagcagcc ctcgcctcgc tcgaacacca cgtcctgacg cgcataggtg
    12001 cccataacag cggaggatgg gccagaggag gtcgtcttgg cgttgggtag gttcgtagcc
    12061 ggggcgggct gcgacatggc tggcagctcc tgttggggag aaagaaaaag agccgccctc
    12121 gtgtggcggc ccggtgacaa tccaatggat actacccgtg ctgaccagct gtcaacgtac
    12181 tggaacgcac gctgattttg ctccgtaggg acacccatgg agccgagccg ccggcaaaca
    12241 tacagcgaac tcggctttgc ttgtggcacg aacgccacga ttcatcgcca ataggccgtt
    12301 gagagagccc tcgtaccttg tagaacgaat tggcgatgat gtagcttgac ccttgcccgc
    12361 cgcgacaagg cggtggcgcg ctgcaaatgc gcgccgggtg agtagctttt cccgagttcg
    12421 gggcatgccc cgcgcgccca gtcggcgcga ttcgtctgag ttccccaagt tccagcgtca
    12481 gtaaacggcc aagctgtgtg ccggccatgc gttgcgccct tcgcgttgcg cggccaacgc
    12541 cgcttgccgc tcgagtacgg tccgccgacc cgcggatcgg atgtgactga ttctgcaagg
    12601 agcgacaaga tgggctggac ggatgagcgc gtcgagcaac tcaagaggct ctgggcggaa
    12661 gggctgagtg ccagccagat cgccggccgc ctcggcggtg ttacgcgcaa cgccgtgatc
    12721 ggcaaggtgc atcggctcgg gctttctggc cgtgccacca cgtcgcgtat gaaatcccat
    12781 cgcgcacggc cccggacttc gcctggcagc gttacgaagc ggccggcggc caaaccccgc
    12841 ttcggaacca tcggcaatcc ggcgctacgg gccctgtacc agcccgacgc cgaaacctat
    12901 gtgccgcccg cagaggagct cgtcattcca cccgaggagc gcaaaacgct gcagatgctc
    12961 accgagacaa gctgccgctg gccgatcggt gacccgcaga cgcccgagtt ccacttctgc
    13021 aatcggaata aggtacccgg cctgccgtat tgcgagatcc atgccaggcg ggcattccag
    13081 ccgccgcagc cgcgccgtcg tgatcgcgag gtcggggaac ccgtcgttcc ggccattccg
    13141 gcccaggccg ccaaggatcc cgtggacgct tgacgggctg cggtacgggc aggagctttc
    13201 tagggaccat cgcatctgct ctgcgcgcaa ggggaattgc ctcggcgcgc aagccatggc
    13261 agaccacatg aactgcgcgt ctgcagtctc gtcccatgta aagcgatcca gggtgctcgg
    13321 atatgaatgc acagcccaac gcgaccttcc tttcgacggt ctggccggaa agccgcgaca
    13381 gctttctgcg tctgggcgcg gtcgtggtcc tcggcacact gctcttgacg gcatcggcga
    13441 agatccaggt gccgttctgg ccggtaccga tgaccatgca aacctttatg gtcctgtttc
    13501 tgggcgctgc cctggggcct cgcctcggcg ccttgacggt gcttctctac ctcacccaag
    13561 gcgcagccgg actaccggtg ttcgccggaa cgcccgaaaa aggattgggc atcgcctaca
    13621 tggccgggcc gaccggcggg tatctcttgg gcttcacggc aggggcgtat gtcgtcggct
    13681 ggcttgccga gcggggctgg gaccgctccg tcctgcggct gttcgcggcg atggcgctcg
    13741 gtcacgtggt gattttcgct tttggcgtgg cctggctgac gcagctcgtc ggtcttgaga
    13801 aggcgtggct gctcggggtc acgccgtttt atgcggcgac gctgttcaag acggcgctcg
    13861 gggcctgcct tatgcctgcc ctgtggcagc ttgcaaagcg ctgacttgag gcataagccc
    13921 aatcgttgaa gcggcgccgt catcgggcgc cgctttcatt cggtgttggt tctgagatca
    13981 ggcagcgcgc cgctcggccg agctgcccgt ctcctccggt tccgggaacg tgatgtaatc
    14041 ccgctcgtgc tcggcgctgc tgctcttggc cttgccggcc gacttctgca ccatttcgaa
    14101 ccaagccccg agaacgcgct gcgagctttc cgtgtactgg cgcgccatcg tttgccaaaa
    14161 gcgcatctgc tcgttcgcca agtcctgagg tgaccggcac tggctcagcc gcatcggcag
    14221 ctccatgtag gcctgtgcac ggcggttcat gagtgtgaag gcctcgatgt tgccgcgagc
    14281 cgccgcacgc aaccacggct cgattgcctt ccccgctgcg gccaatccgc tatacgcggt
    14341 gttcagcata ccttggatgg gcccccccga cggatcccta ctgttatcca tacgcatgac
    14401 cctactccat ttcgctgacc agaaaccaaa ttatgcttta caagtggcga tgatacaaca
    14461 cgcatctagg ctaaaattga tgcagcgcaa caaagctctc gcttttcgga atggcacgac
    14521 ggacggttaa ccttactcag ccttcgatca gtccgacgag cgtatccaag cgctctgcct
    14581 cttctgccgg cttatcccag cggatgcgat gaatgcgggg aaaccgcatc gccaccccgg
    14641 agcgatgcct ggtcgaacgc tgtacggcat cgaacgccac ttccagcacg agggccggcc
    14701 tcacggcacg tacgggacca aacctctcga cggtgttgtt gcggatccag cggtccagca
    14761 cgatcagctc ggcgtcggta tagccggaat aagccttgcc gaccgggacc agctcgaggc
    14821 tgccatcggg cagctctttc catgcaccga aggtgtaatc ggaataatag gatgaccgcc
    14881 ggcccgagcc gcgctgggca tacatgagca cgcagtcgag ggtgatcggc gctcgcttcc
    14941 acttgtacca gtgcccgcgc ggccggccgg cgaggtaagg gctcgcccgg cgcttcagca
    15001 tcagtccctc gatgccggca cctcgtgtct cgcgccagat ccggtgcagc tcctctttgt
    15061 cagcgaccgc gatcagctcc gacacgtcag tccgcggcgg acgaacctcg gcgtgccagc
    15121 gctcgagccg tcggcgcctt tcgatgaacg gcagggggcg gagatcttct ccgtcgatga
    15181 gcaaggcatc atagagacgc acgtgagccg ggtagcgctc cagcatcgcg cgggtcacgg
    15241 acttccggtt gagccgctgc tgcaagtcgt tgaacggcgc caccttgcca tcccgcagca
    15301 ccagaagctc gccatcaatc accgcgtcga agcggaacat cgagatgagc tcgggaaagc
    15361 tcgcgccgat ttcatcgccc gttcgcgaat agagccgcac ctctccgcgt ccggccacga
    15421 tctgtacacg gatgccatcc catttccatt cgacggagaa ctcgccgata tcgagatccg
    15481 caagctcgga gtcctccagt gcatgcgcca gcatcaacgg ccggaagaca ggcgcgcccg
    15541 cgatatccgg ccgctcggca tggccctcga gccagtcgaa gagcggctga tagggtggcg
    15601 acagcgcgtg ccagacctcc tcgacgtcat cgacgctgtg ccccgccatg cccgcaacgg
    15661 cggttttggc gaggcgcgcc gagacgccaa cgcgcagagc gcccgttagc agcttgagca
    15721 gcgcccagcg gccggttgca tcgagacggt cgagccagga tgcgatgacg tcgcccagct
    15781 cctgcgcgtc gatcaaacca aggcggctga cgacctcgga caggcgaggc gggtcgcctt
    15841 gtgcctcggt gtcgggccac agcagggcga ccgtctccgc cgtgtcgcca acatagtcgc
    15901 gcgacagacg aaacagctcg gcgtcgagcc gcttgtccat cagttcaacg atggtccggc
    15961 gcaccggaaa cgaaaacggg agtccgtcgg tcagcgccgc cagcgcccag ccccggtcgg
    16021 ggtctggcgt ggtgcggaag tactcctgca tgagctgcag cttcgtattg cggcccggac
    16081 tgtagacgag ccgatcgagt agcgcggcga aggccttcat gggcggcctc gctttgccgg
    16141 aagcagagcc gcccaaaaga cctcgccttg atgggcagcg gacttgatcg agtgactcaa
    16201 tcctccgcct cctcgtcctc ccgtccaagc aaggccagag cgcgggcgcg cttgcccatc
    16261 cgcccgagct ggtaaacgag ggcctcctcg cggccatggg tgacccacac ctcctctgcg
    16321 cccgtctcag caatggtctc gatgagctgc ggccagtcgg catggtcgga gatgacgagc
    16381 ggcagctcga cgcctcgctg gcgggcgcgg ccgcgcacgc gcatccatcc cgacgcgaat
    16441 gccgtcaccg gatcggcgag tcgtcgcgac cagcggtcct tgagggcgga cggggggcag
    16501 agcacgatct cgccggcgag gccgcgcccc gcctcgctga ccagcacgag ctctccgaag
    16561 ctctgcccgc atgcgatgta gagatcggtc atgctcttca gcgcgccatg caggtagatc
    16621 ggccggtcgt agccggcgcg gcgcagcagc gcgagcagcc gctggcattt gcccagaccg
    16681 tagcacccga tgaggtgcgt gcgttcggga aacgcctgta ccgacgcgag cagccgcccg
    16741 atttcacgcc aatccggctc gtgccggaaa accggcagcc cgaacgtcgc ctccgtgacg
    16801 aagaggtcgc agggcaccag ctcgaagagt gcgcaggtgg gatcgggcgt gcgcttgtag
    16861 tcgcctgaga cgacggcgcg ctgccctcga taggtgagct cgatttgcgc gctgccgagg
    16921 atatgaccgg cgggatggag cctgatcgtg acatccccgt gcctcagcgg atcgttcagc
    16981 ggctgaggtt gaaagcgccc tgccgagtcc ttgcccaggc gtgcctgcat caccttgacc
    17041 gtctcgggtg tggccagcac ggcgccatgg ccgcctcgcg cgtggtccga gtggccgtgc
    17101 gtgatgacgg cccggtccac ggggcggctc gggtcgatgt gaaacccgcc gggagcgcag
    17161 tagagtccgg cctcagtcag ttgcaaccag tcttcgaatg cgatcaacgc gtgtagttct
    17221 ccagataccc ccgttactta ccctcattct ggttgaatat aggcggaatt ttcaatcaaa
    17281 tatcgcatcg accacgggtt gaacttgtcc agaactgcgc caacccgctg aaaatgtcgg
    17341 agtcatcatt cgttttcagt tgaggccaag ataaatgttt ggttaacatc gttcagtaac
    17401 caagttggcg cgagtacggc gcgcgtgtat cgagttcgta atgtgggccg gctaatcggc
    17461 agttcgaagg tcgcgtggcg cgggggcacg atcgggctcg gggcggcttt tgttctcctt
    17521 gcctcaccgg ttgcggccca gcaggccaag cctactcagg aagtctgggg aggcgccgat
    17581 gtcactacga acagttggtc ggtatattcg gggctgacgg tagcgctttt cagctcgctt
    17641 tatgacaacg gcctccgtct ccgcgccgtc ggtggctatg gcttctattc ctaccccaaa
    17701 tacgatgtca ctattcgcgg ctacgtcggt ttcggcgacc ttctcctggg ctatcagcat
    17761 cagttcggac cgctgacgct gaaggcgttc atgggcctct cgacagagtc gcacaatctc
    17821 gcgccgcccg atcctgacag ctcggtcgtc ggcgcggatc tcggggcaaa agtcgccctt
    17881 gagtcatggt gggaaatcgg gccgaagacc tggtcctcac ttgatatatc ctggtcgact
    17941 gttcatggcg ggacctatgc cgcgcgtgcg cgcgccggct accggctgag gccgcagctg
    18001 tcggtgggca tcgaagcggc agccgacggg gacgccgaat atgatggcgg gcgtggcggg
    18061 ggcttcctcc gctacacctg ggcggcgggc gagatttcgg ccagcgcggg cgcgtccatc
    18121 gacagatccg gcgagagcgg cggctatggc gcgatgaacg ccttattccg ctactgattt
    18181 cgctcattcc gcgcgcgttt gccacgctgc agcgacattg tctggcatac tgcgaatcac
    18241 cgccgcgccg gattgtggcc ggccaatcat ggtactatcg cgcgtccgtc gaagcccaca
    18301 aacaacatca aagcggaggg caccgtgaaa agcatcctcg ttcccatcga gaagcacgat
    18361 ctcgtgacct cgatgctgga ttgcgcaagc cggcttgctg gccggttcgg aggcatcgtc
    18421 gagggcgttg ccctgcggct accgcagatt tccgtcgtcg ggcccgaccc ggtcgtgacg
    18481 gtgacgttcc cgcgggccga gcaggaagac ctcgagatca tttcgacggc gcggcgcata
    18541 ttcgacgcgt atttcgccag cgaaactgcc gagggcctcc ccgagagcca gcgcccccgc
    18601 caccgttggc gcgccgtcga tccggtggac gatgctgcat tggcgagcct cgcccgggtc
    18661 tatgacctga cggtcgtcgg ccggcctcag gctggaacag cagggccgcg catgacgacg
    18721 ctcgaatcgg tcttgttcga aagcggccgt ccgctgctga tcgcaccacc gcaggcgccc
    18781 cgctcgctgg gcgatcgcat cgtcatctcc tggaactgca gttctgagtc ggcacgcacg
    18841 gtggcttatg ccatgccgct gctgctcaag gcccgcgagg tgacggtatt gaccgtcgaa
    18901 ggcgcggttt cgcccggacc atccggcaag gagctggccg attgccttgc cgtgcacggc
    18961 atcgaggccc gggagttgac cgcattgccg ggagggcgca agcccggcga ggccattctc
    19021 gaggaagccc agcggctcgg cgccgacctg ctcctcaagg gcgcctatac ccaaagccgg
    19081 ttacggcaga tgatcttcgg tggcgcgaca agccacatcc tgtcgaacac cgagctgccg
    19141 gtcttcatgg ctcactgacc tcccgcctgt gactggggtg ggaatccgat ttgtcagatc
    19201 cgcttggagt acgccttcgc cttaacggca cgagaggttc ggccagctcc ggctcggcca
    19261 gcgcacgagc gctgtataac gcaggacgac ggctggagca tcctcggcag gtgccagacg
    19321 gctcacctta cgaggcgtcg tcaggctcaa cctcggtacc gcgcgaactg gccatacggt
    19381 ctgaacggct ccagatagtc tgggacgatc aagtcgatcg tgtgcagttg cgtcacgccg
    19441 aggccctgca gggtgcggcc ctcctcttgg gcggcagcac tcaccacatt gtccatcctg
    19501 agcagccgga tctgatcgag cgtgatcggg cgaatgctgt tcggcagcgg ccaggtcagc
    19561 agggcgacga gctgcgccag ccagaagggg atgggcaagt aggggcggtg aagatccgtc
    19621 cattccttca cccgatccag caattcgcgg aaggtgaaga tctcgggccc tcccgcctcg
    19681 tagaccgttc caggcgtgcc agtcccgttg acggcgttgg cgatggccct gccgagatcc
    19741 ccggcgtaga caggctggaa gcgggtgcgc ccaccgccga tcagcggcag aaacgggctg
    19801 aaacgtgcaa gagatccgaa tcggttgaag aagtcgtcct ccgggccgaa cacgacggag
    19861 ggccgaatga tgacggcctc agggaactca tcgagcacgg ccttctcgcc ctccgccttg
    19921 gtgcgggcgt aggccgatcg gctggccgga tcggcgccga tggccgagat atggacgaac
    19981 cgctgcgcac cagcctgtcg cgctgccttg gccacggctc gcgagccctc gacatggaca
    20041 gcatggaagc tctgccgtcc tgatgggatg aggatgccga ccgcattcac gacggcgaca
    20101 gcgtccttca cggcatgctc gatcgactcg gggaagcgca cgttggcctg cacggcgtgg
    20161 atctggccga ggcggcccat cggctggaga tgccccgcga gatcgggacg cctcactgcg
    20221 gcgcggattc gccagcccct ttttgccagt tgctgaaccg cgtggcggcc cacgaacccc
    20281 gatcccccaa agacggtcac caaaccgcca aatgcgaggt ttgcggacat gcctctcaat
    20341 ctcctgccgt tcgtccttca cgcgatgggg agcaatacac aacccccctg ccgctgtgaa
    20401 gcgacgcggt gcaattgaca atggaagcgc tcggctctaa ctgatggccc acctagccca
    20461 ggtggcggaa ttggtagacg cactagtttc aggtactagc gggtaacacc gtggaggttc
    20521 gagtcctctc ctgggcacca cttgctaaga agatcttcct tacttttttg gtgttttctt
    20581 tcccaccctc cgcttcccca tacccaaggt ggaaaagttt ccggcactgt ttgttctcga
    20641 gagctggtga ttgtgatcca aggcgagtcg cttgcaatca gccgcttcgg tatagagctg
    20701 ggcttgcttg agcgtacgcc aaccgaagat cgccatcagc gcgtgagtgg atgcacctct
    20761 ctcagctaag tacgtcgccg cggctttgag cgagccatgt gcggagcggt cgaccagccc
    20821 tgcttcgggc accgatcgcg aaaccaattg gtgaaccctg ccgccgtgag tgtttgacca
    20881 cgctccgtga caaggaatgt caggtcacct gtggggccct catccaggat ctgctcctgt
    20941 tcgggcagca ccggaacgag gtgcctagct tttcaggaat gcgagtacga ccacagcgcc
    21001 ccctcgacgg agcccgctca gatggcgttc gcattggatt ctcaacctca ggtgggggga
    21061 tacccaatgc caaaaccggc gcgcacacag tgcggccggt cctgatcatt gggtcatttt
    21121 gggggtgatc aatatgtagg cgcgggtgcc gagtgcactg tcactggagc agcctcacat
    21181 ggtaccccga gccaatcaca tggacttatt cagatggcaa cgttttcttt gacgacgctg
    21241 aagcaggttg gcataatgtc gtacgaaatg tggcttggct aggggagccg atgcgaccgc
    21301 gcggccggtt tgagtgacga gcgaccctat agcttcgcgt atgcgttgcc ggcagtggac
    21361 aggcccgagc agggtttggc gctgtcagcg ggtacgtagc tgaagcgtgg atttcacctc
    21421 gtcgcagagc cgggcgttcg tcgttttacg tccgcctatt taacgacgcc gctcttcgct
    21481 ggaagcattg tatcctccga cgccagttat ttttaatttc catgccgatc agcttggatt
    21541 gaaacgcaat gcagttgttt cgggatcatt tgttgcatag accgcatgct gccgaatgcc
    21601 ggatttggtc ccgtgctggt cctgctgatc ggctaaacgg ctgatccaag ccggagggca
    21661 accaacaaga agacgtgggg gagggacaat gccgatttca tggcaggcga ccgttcggcc
    21721 tgcgttggca gccgcaatcc tagcatttct cggcggcgcg gctggggcag agacgatcac
    21781 gttacgtggc ggcgacgact tggcggaagc ggtccggcgg gcagcggacg gtgacgtgat
    21841 ccgtctggaa ccgggacgct attccggacc tgtcattgtt gagggcaagc gcatcgtcat
    21901 cgaaggccct tccggcggtc aggctgtgat cgtgccgggc cgatcgaatg tgctggttgg
    21961 ggcacagaac ggcggacagg tcgaactatc cggcttgaag ttcgaaacca gcgccgagac
    22021 gcccatggcg cttttcgcca agggcggcgg gataagctgc gccgattgca cgatcgagtc
    22081 ggcgcagaac cagccggttt acgtcgaaag cggaacgttg gcgctaaaca gcagcaaagt
    22141 cagtggcgcg gggaaccatg ctattgccgc cagggacagc gcaatcacca ttgagggcac
    22201 gcaggtcgag
//
LOCUS       DASVDU010000122         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1384420, whole genome shotgun sequence.
ACCESSION   DASVDU010000122 DASVDU010000000
VERSION     DASVDU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384420"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VF226_07855"
     CDS             complement(<1..687)
                     /locus_tag="VF226_07855"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX7073938.1"
                     /translation="MLWRKDEAHAESALWSLLLNGAEGLFPGYFALVMATGVVSIASH
                     LLAISVIPKVLLAINWVAYAVLWFLTGLRVVRFPARVIEDLSKHHRAPGFFTIVAGTC
                     VLGTQSVIVAGATDVGLALWWIGLVLWCLVMYAFYTAVTIRKRKPSLAGGINGAWLIA
                     AVATQSIVVLRGAVDAAAAPSAAIQFLCLAMFMIGCMLYLAIIPLIFYRLTFIKLEAY
                     EFEPPYWINMG"
     gene            complement(968..1669)
                     /locus_tag="VF226_07860"
     CDS             complement(968..1669)
                     /locus_tag="VF226_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7073939.1"
                     /translation="MMDPEDWQVVKSTPLFGAMPMDVTVSLVGSAGPRSYEKGTLLFQ
                     QGDPASAFYVILDGWVKLARITPEGSEAVVGVFSRGETFAEAAMFLGGKYPVNAEVVA
                     PSRLLKIDGEALRRKIRENPDLALSMLASASHQLKSLVEQIEQIKLLSAPQRIADFLI
                     QQCRTRQGSCVVELPYEKALIASRLGMKPESFSRAIAKLRSLGVSVDHEFVSIADVGA
                     LIGFVEKSAESYDES"
     gene            complement(1863..2762)
                     /locus_tag="VF226_07865"
     CDS             complement(1863..2762)
                     /locus_tag="VF226_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7073940.1"
                     /translation="MFKPAKITLGPVLFNWQPEAWRDFYARIADEAPVDQVYLGEVVC
                     SKRMPFFDELIVDVIERLERAGKSVVLSTLIMPTTSRERRLMRDLVSGFDQLIEVNDI
                     STLSHLNGRPHVVGPFINTYNESMLAYLARRGASAVCLPPELPLASIRCIAAANPDVA
                     IEVFAFGRSPLAISARCYHARLHGLTKDGCQFVCGEDPDGLDVDTLDGQPFLTVNGVQ
                     TLSRTYTCLVGDIPQLMEAGVSAFRLSPHTCDMVAVSRLYRDVADGRSEPGAALEQMR
                     RLVPGAAFSNGFLHGVAGVELVS"
     gene            complement(2769..>3594)
                     /locus_tag="VF226_07870"
     CDS             complement(2769..>3594)
                     /locus_tag="VF226_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX7073941.1"
                     /translation="TYAHARGCKVLVAINTFPRAGHVALWQRAVDDAVGLGADALILC
                     DIGLLDYAATKHPKARLHLSVQAAAANPEAIRFYTASFGVKRVVLPRVLTVSEIASIN
                     NDIDCETEVFVFGGLCVMAEGRCSLSSYATGKSPNMNGVCSPPSHVSYHEEGDELVPR
                     LGGFTINRIPRSEPAAYPTLCKGRFVAGTSSGYIFEDPVSLNAGTLLPGLVKAGVTAL
                     KIEGRQRGRAYIAAVVKAFRQAVDALAEGRSVDLDRLLALAEGQSSTQGAYAKTWR"
BASE COUNT          721 a         1121 c         1140 g          612 t
ORIGIN      
        1 gcccatgttg atccagtagg gcggctcgaa ctcgtaggcc tccaatttga tgaacgtcaa
       61 gcgatagaag atcagcggaa tgatggcgag atagagcatg cagccgatca tgaacatcgc
      121 caggcagagg aactggatgg cggccgacgg ggccgcggct gcgtcgacgg cgccacgcag
      181 cacgacgatc gactgcgtgg cgacagcagc gatgagccac gccccattga ttcctccagc
      241 cagcgatggt ttgcgcttgc ggatcgtgac ggccgtgtag aaggcgtaca tcacgaggca
      301 ccacagcacg agcccgatcc accagagcgc caggccgacg tcagtcgcgc cggcgacgat
      361 cacactctgt gttccgagca cgcaggtgcc tgccacaatc gtgaagaagc ctggggcccg
      421 atggtgcttg gagaggtcct cgatgactcg tgccggaaag cggacgacgc gcagtccggt
      481 caggaaccac aggacagcgt acgcgaccca gttgatggcg aggagcactt tgggaatcac
      541 cgatatcgca aggaggtgag aagcgatcga aacgacgccg gtcgccatga ccagcgcgaa
      601 atagccggga aagagacctt ctgcaccgtt cagcaaaagg ctccacagtg ctgattccgc
      661 gtgcgcttcg tcctttcgcc aaagcatgtt cgcatgccct caccgtccca ctgcgccgat
      721 caagaccaat acgggatcgc gtgattcaga tcgagaagtc ccatgggcag gcctcgttcc
      781 gtgaagcccg cctcagaccg ctcatgacgg gttgcgacag cttgaccgac gccggcggtg
      841 cttccttgac gccggttaaa cctgaccatg aattgggcca agagaataat ggcgctcccc
      901 ggcagggaat agctccgcgc aaggccgacg tcgccaagtt tttctctgcc gggaaggcgt
      961 cagggtttta gctttcgtcg tagctttccg cgctcttttc gacgaagccg atcagcgctc
     1021 cgacatcggc gatcgagacg aactcgtgat cgacggagac accaaggcta cgcagcttgg
     1081 cgatggcgcg cgagaagctc tcgggcttca tgccgagacg gctggcaatc agcgccttct
     1141 cgtagggcag ctcgacgaca cacgacccct ggcgtgtgcg gcactgctgg atcaggaagt
     1201 cggcaattcg ctgcggcgca gacaacagct tgatctgctc gatctgctcg accagtgatt
     1261 tcaactgatg cgatgccgag gccagcatgg acaaggccag atcgggattt tcgcggatct
     1321 tccggcgtaa ggcttcgccg tcgattttca gcaggcgcga gggcgcgacg acctctgcat
     1381 tgacagggta tttgccgcca aggaacatgg ccgcctcggc gaacgtctca ccgcggctga
     1441 acaccccgac gaccgcctca cttccctccg gcgttatccg ggcgagcttc acccagccat
     1501 cgagaatgac atagaaggcg ctagcaggat caccctgctg gaacagcaag gttccttttt
     1561 catagctccg cggacctgcc gaacccacga gactgaccgt gacgtccata ggcatggccc
     1621 cgaacagtgg cgtcgatttc acgacttgcc agtcctcggg gtccatcatt ggggcgctgc
     1681 gggccagctg tccacactcc accgcttccc tccagagcct tacgctgaag agtcatgcgc
     1741 tgaggttctg cgccagggcg agcagattga ctttgatgga gatcaaagac tttgcacgta
     1801 ggtgccgtgg agttcggcga cagaggtgga aatcttcaca ggagcccgct cggggctcgc
     1861 gatcacgaga cgagttccac acccgcaacg ccgtggagaa acccgttcga gaatgcagca
     1921 ccgggaacga ggcgtcgcat ctgttccaga gcggcgcccg gctctgagcg cccatcggcg
     1981 acatcgcgat aaagcctgct gacggccacc atgtcgcagg tgtggggaga aagccggaag
     2041 gcggacacgc ctgcctccat cagctgaggt atatcgccga ccaggcacgt gtatgtgcgc
     2101 gagagggtct ggacgccgtt gacggtcagg aatggctgcc cgtcgagcgt atcgacatcg
     2161 agcccgtcgg gatcctcccc gcagacgaac tggcagccgt ccttggtcag gccatgcagc
     2221 cgggcatggt agcagcgtgc agaaatcgcc agcgggctgc ggccgaacgc gaacacctcg
     2281 atcgccacgt ccgggttggc tgccgcgatg catcggatgg aggcgagcgg cagctccggc
     2341 ggcaggcaga cagcgcttgc accgcggcgc gccaggtagg ccagcatcga ttcattatac
     2401 gtgttgatga aggggccgac gacgtgtggc cgcccgttga ggtgcgagag tgtcgagatg
     2461 tcgttgacct cgatcagctg gtcgaagccc gagaccagat cccgcatgag gcgtctttcg
     2521 cggctcgtcg tcggcatgat cagtgtcgag aggacgaccg acttgccggc ccgctcgagc
     2581 cgctcgatga catcgacgat cagctcgtcg aagaagggca tgcgcttcga gcacaccacc
     2641 tcgccgaggt acacctgatc caccggggcc tcgtccgcga tccgggcgta gaaatcgcgc
     2701 catgcctccg gctgccaatt gaagaggacg ggcccgagcg tgatcttcgc gggcttgaac
     2761 atctgcacct acctccaggt cttagcgtac gcgccctggg tcgacgactg gccctcggca
     2821 agcgcgagca gccggtcgag gtcgacggac cgcccctcgg ccaaggcgtc gaccgcctgc
     2881 cggaaggctt tcaccacggc agcgatgtag gcgcgcccgc gctggcgacc ttcgattttc
     2941 agggcggtca caccggcctt gaccagcccg ggcaagagcg tgcccgcatt gagactgaca
     3001 gggtcctcga agatgtagcc ggatgacgtc cccgctacga aacggccctt acacagggtc
     3061 gggtaggccg caggctcgga gcgcggaatc cggttgatcg tgaaaccgcc aagacgtggg
     3121 accagctcgt cgccctcctc atgatagctg acatggctcg gcggcgagca gacgccattc
     3181 atgttcggcg acttgccagt agcgtaggac gacagcgagc agcgcccctc ggccatgacg
     3241 cacaggccgc cgaagacgaa cacttccgtc tcgcaatcga tgtcattgtt gatgctggcg
     3301 atctcggaga cggtgagcac gcgcggcagc acgacccgct tcacgccgaa tgatgccgta
     3361 tagaatcgga tggcttccgg gttcgctgcg gccgcctgca ccgaaaggtg gagcctggct
     3421 ttcggatgct tcgtcgccgc gtagtcgagc agaccgatgt cgcacaggat gagagcgtcg
     3481 gcgccgagcc caaccgcatc atccactgcc ctttgccaga gtgccacatg tcccgcccgc
     3541 gggaaggtat tgatcgccac gagcaccttg cagccacggg cgtgggcgta ggtg
//
LOCUS       DASVDU010000123        19289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1600929, whole genome shotgun sequence.
ACCESSION   DASVDU010000123 DASVDU010000000
VERSION     DASVDU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19289
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600929"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..6986
                     /locus_tag="VF226_07875"
     CDS             <1..6986
                     /locus_tag="VF226_07875"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HEX7073942.1"
                     /translation="SGNNASDTDDGELFYEPGGNEHNDPDIGTLVATSDTAFVFGLEG
                     HDTLIGNNIAGLTLDGGVGHDVLDDGDQIEAGAKLYGRAGNDILFGRGGDDYIDGGDD
                     SDFATGDAGDDLIFGGAGADWLDGGTGKDIATGDAGEDKLFGGAGHDTLVGGDEADRL
                     YGDTDFGQIFLVRYQGDIGVHRSDDPNVAGLFPLFRDAPLAEAGDDYLEGGGGNDELF
                     GGAGHDTLLGGADDDVLQGEGGNDRLIAGSGDDTLWGDADPATAKADGETAITAGYYE
                     YFTRARDVGADGDDYLDGGSGDDRLHGGLGDDTYVFGWGYGEDMVFEAGGYDALLLES
                     RIRPDDLAFEKVGAALIVAVSGDDRAILTNWFGAASVVESSRIEAIRFGGGTFWDTAE
                     IERRSGLYNLSGEGQVGDTTTNGTDYYASFGLDVDDGFDTVIEDAGGEDTLHFDDVLF
                     AQTPLGPLYIRPILEEALRDENDLVLRVFLDSDIERPDVRGTVRIRGHFAEGQVERIS
                     LAGGRVLVAPEFALARPGTYLQRDHDAILYETFDPLASYPHVEAFYHVGDDGDDFLQL
                     SPGTSADVYHYYGNGGDDVLTGSAGSDVLVGGAGADRLEGSYGSDVYHIDPGEQDVVL
                     DDERSYAGSSTDGLVLPVGLTLSEIAFGREGEDLFIGSTRVERYFERDLSHAGGSDYP
                     FLVEILQVGELHVDLPEHLIDLGLFGTYSGTDDAETLIGDRYDNVLQGAGGDDVVYGW
                     RGADRILGGEGDDELYGEQDPALLIFRHTNDHISGGSGNDYLQGDWGSDILLGDEDND
                     VVYGDEGLAELPDWAIGNIFIPYGRDTLAGSSGDDFLYGEQDGDTYRFGIGDGVDVIQ
                     DWGYDRWNNDHTLGGETLLDVHRAIIRLDYFEGATYRPPIFVPFREHRLEWAVPDDLL
                     VEVEALEDGLLAPEAARVTLERLREWYIDDQLDVVDFGAGIAPENLAVEVLPGELLIA
                     IGGNDSLSIRDPAGGSNFGIERFRFADGSELTLADLMLLDESIDHEIQAEGTFGDDLL
                     IGGDSSDFLDGRGGNDTLRGRAGDDRLLGGSTDTNTFGLVPNADILDGGAGNDYMDGG
                     DGADTYIIRANEGIDIVEDGGDEFDTAIIEGAIQELVDFEGETYSNAYWAEELDEWAA
                     FSISQELGRAVLALQHGVLPSVALQTLIDLRAWFLGLHADAIEFGEGITPENITFHWN
                     DSGEVFVGTSRSHGVRAQGIESLRFADGQEVALDELRDSSNRISGDVVTTQGSDAPDV
                     LAGYFIDDLIAGNGGDDILVGYSGDDRLSGGDGDDILVGGRSTSGNWLEGGAGNDVYL
                     FNRADFAIGEILDTGNPGDLPDSVVERVGDLNPDPSASDIDTFSFGSSISPQDIRAYA
                     RMYENPGGSVALLALTLDGAEQGIFVVWGLDAGQGVAHIGAIERAQFIGQGAHKVFDL
                     VGIVQTRFDELLAASSTDPIPLFTPEVLASFEISDSVPLAGGSHAVNYALTGDPFIEP
                     PNEAPAVAKPLPDQAVHQGDEVRFVIAPDAFADPESQSLVYAAMLSDGSPLPAWLAFD
                     SETRTFEGTPENNDVGTYLIRVLASDTQGLAAFDEFLLVVHDVNDAPVASSTLPDQTA
                     SEDHLVSFGLPEQAFFDVDVIHGDGLMYSAALADGTALPPWLAFDPLTRTFTGKPANA
                     DVGVIEIRATATDAAGLSASSDFTLTIANVNDAPLAVGSVDDQVALEDTPFGLFVPES
                     TFADDDLVHGDELAYTAQLADGSALPGWMTFDAATATLSGTPENSHVGIYEIAVTAVD
                     RAGASAETVFQIEVVNVNDAPVTQAGVDDQEAVEDAAFVFHLPSAAFEDVDVVHGDSL
                     RFDAVLRDGTALPAWLGFDPATQMFFGTPANGDVGRIEIRVTATDTGGLSSSDAFTLT
                     VANTNDAPFIVSALGDRHATQDVPVGLPLAQAFGDIDMIHGDALTFAATLAEGGSLPE
                     WLDINPHTGVLSGVPGNDAVGQLSIEVTATDAAGAAVSALLGLTVHNVNDSPVVALPV
                     ADQSVNEYEVVRYQLSPQVFADPDAMHGDVLTYSAMLGDGSPLPGWLSFDPVGRTLMG
                     LPSFSERGDHQIRVSATDAAGASASVTFTLSVHGVGNAISGGPGTDILNGTGGNDVIF
                     AGGGFDLLSGNGGDDILDAGDGNDLLFGGSGDDRLLGGAGDDLLYGGNGNNRLHGGSG
                     NDLLAGGADADVYVFGRGDGEDTVLALGSPRSASDAVHVGANPLDILFSTARASLQVD
                     LHGSDDRLSVTAWRAPANQIETVVAQDGSTIYAAQIEQLIQAMNQFSADNGGISWEQA
                     VAERPQDVEAVLAAYWQPAAA"
     gene            complement(6983..8368)
                     /locus_tag="VF226_07880"
     CDS             complement(6983..8368)
                     /locus_tag="VF226_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX7073943.1"
                     /translation="MGKSLIRDVGGHDSRKHGATLFSALALGCLMALPSALRAEDLLS
                     IFRLASENDPQVRAAFARYQAVQQAVPQARAGLLPQVNATLARNRNAEEVSTDAFIFS
                     RPAGRASYYSDSYEVNLSQVLYDRALWTRLDQASAEVRRAALEYRAARQNLILRVAEA
                     YFRVLLAQETVALAEAERQALARNLEAAEGRRRAGSGTIIEVHEARARFQVSAAQLIE
                     ATNELADSREALREISDRLPGNLQGLGDDTAFQAPEPAELSAWIETALQQNSEVLAAR
                     EALEVARLEIDRNRAGHFPTLEMVGTHSRHDADASIPGPGIRADDTLIGVQLTIPLYQ
                     GGAVSARTEEAAHRYGAAREELEARRRAVERSTRAAFQGVMASLARIDALELAINAAE
                     SALQAKTEGRGFGLYTTLDVLDATRELYQARRDLAEAQHTYLSNLLQLKYAAGVLAED
                     DVLAVNAWLRP"
     gene            8714..9919
                     /locus_tag="VF226_07885"
     CDS             8714..9919
                     /locus_tag="VF226_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073944.1"
                     /translation="MTGALVWNFRAELRADTVKAPWVGVFVLAGLWLLYLAGKSWLQW
                     RRFGTLPLTLDPYRVTVGGDLGGTIEIPGLRATYDTPFEVTLSCVRVTIRRSGKSRKR
                     NERVEWRERGTVTGAPGVRGTRLQLRFAVPGHLPATTPPSRDYHKWVCTCTPRLPGSI
                     WIRRSRCRWSRPPWSCNDRLAVSSTRAPRSARRWRPGTARQRRAPAWRSATALRGCDF
                     SIRAPANAAWASGSILGLVFGGVPLFFVSQIQSATAGDAFRFGRDHELRGRDVRPVRA
                     PAHRIYLIRNSLEVIVSTHSVDTVRRLYGVPVHRRHALRESITGLQSKIFGPSGQGTK
                     AKVSYTIKAVLSDGRTLAVGDGIRGVTEAKRVLARLREHLGLPARKQSCVRLPRQGGQ
                     RSAPTDRAR"
     gene            complement(9984..10727)
                     /locus_tag="VF226_07890"
     CDS             complement(9984..10727)
                     /locus_tag="VF226_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073945.1"
                     /translation="MTNRTLLARSKIPLYLQVAAQFRLRIENGMWSPGQQVPVLEALQ
                     AEFGLARVTIRQALEVLEQEGLIRRHRGRGTFVTEHLPARQEHVLPASWEQLVASLHP
                     VKLSVLAGPRQVAAITPSPHFPLSEGFVPCHVRRLHSIGSAPYCLIDIYIDKDLFYSN
                     EKKFMSQPMILVLNDDHRGLVHEVRQVVTFSISDDVTSEALNITLGEPVVKIERHIAD
                     SRGNTVLYSRAQFSGVYVRIEQNLSPATR"
     gene            complement(10782..11543)
                     /locus_tag="VF226_07895"
     CDS             complement(10782..11543)
                     /locus_tag="VF226_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEX7073946.1"
                     /translation="MSISNEFKKRLLAGETLIGSWCMAGSASIVETMGWTGFDFLTID
                     GEHGPYSPADILDSLRAIAGTPSLPVVRTPGHDATYIKRLLDIGAQTLLVPMVETADQ
                     AKAIVAATRYPPAGTRGFALMHRASRYLQVEDYAHVANDHICIVAQLESPGAIDRLEE
                     IATVPGIDAVFIGPGDLSAAMGLLGQPTHEDVLSIMKEAASRCARIGVPCGTLFNRAD
                     VARRCLEFGYTMVAVGSDLAFLANSARALAQSMKQ"
     gene            11785..12699
                     /locus_tag="VF226_07900"
     CDS             11785..12699
                     /locus_tag="VF226_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7073947.1"
                     /translation="MRLGSFQRNTGEQGPRVGLFLDDGVVDVGRANPDIPSDVRGILA
                     GGERQLAALEAILSQHQKGALPQDAFVPFDQLRFRPPVRPGKIICVGGNYEDYRRILK
                     LPDLPVPAFFLKSPDAVIAHDEEVQIPEGYGIFYQEWELSCVIGRQCRRVSEQEARDC
                     IAGYTIVNDITGHTLETLGPRPYHMLGKNMDTFAPIGPWIVARDSLAKDVYQLRARRW
                     RNNELVCESSTREMRRSFEEIIVYVSNFMTLYPGDIVTGASPPAGPIEPGDVIEVEFE
                     AVGRLRNTVGRADADPIYGERIGIASGK"
     gene            12784..13779
                     /locus_tag="VF226_07905"
     CDS             12784..13779
                     /locus_tag="VF226_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073948.1"
                     /translation="MRKFLLVAVSLVILAGAGSATADTVLRFAHVNSPNSPAGKAAEY
                     FQERVQLHTEGRVRVQLFPASQLGNNRQMFSSLRGGSIDLSLTPYPMLADIVPELSVY
                     VAGYMFESFEEQQRILQHPELGASWADQLREKGGLRVLCSFYYGSRNLTTTNKPVRTR
                     KDMQGLKIRAVPQPASMSVIRGLGATPTPVPLAEVFQGLRQGVIDGQENPLPVIYNQK
                     FFEVQDYLMLTNHQLVPLPYVMNEAKWKSLGRDQQAVQRAADEACARGTELTVQQERE
                     LIETFKARGMNVITAQDGLDIEGFRASVREEVQKTFDGEIWPAGTWAKIQSALGR"
     gene            13848..14363
                     /locus_tag="VF226_07910"
     CDS             13848..14363
                     /locus_tag="VF226_07910"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7073949.1"
                     /translation="MGPEAEVEKSDEERLAEEGGVLRNLEINIAALALVAFIVLASAQ
                     VISRYGLNRPIPWIEEVSAILLIWMTFIGAAWLVRKDRHIRVELLEEWVHPRVCAVIY
                     TVYDIISIAFLILLAIGGFQLVGAMDFERTPALQIPYRYIVSVVPATALLMAAYFVVI
                     IYRRIAGQKKN"
     gene            14367..15659
                     /locus_tag="VF226_07915"
     CDS             14367..15659
                     /locus_tag="VF226_07915"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7073950.1"
                     /translation="MDPLLIGLVVVSLVLVLGGYQVAFALGACSLVYLIINDFPLTLA
                     AQELALSLDKFTFLAIPLFMLAGMIMNAGGITERIFTFARLAVGRIPGGLGHVNVLAS
                     LVFAGMSGSVLADVAGLGSMEIQAMRKSGYRSDFSIGITLASSAIGPILPPSVPMVLF
                     GVIADVSVTGLFLGGVVPAFVIAGMLMIYVYFVGKREGYFTTAWEGWRPLAVAFVVAF
                     PVLMTPVIIVGGMSLGIFSPTEAATATVLYAFFLAAVVYRDLPWKRVPAIFRTVAIST
                     TKLLFVITCALLFGWVLTVGQVPQTLANALGETFDQAWVFLLVVNITLLVLGAIIENA
                     ILLMVLGPMLIPIGESIYGLDPIHLGVVMVFNIMIGQYTPPMGLSLFIVRDITGQSLA
                     QVSWAVFPFLLPLIAALLLVTYVPGLVLWLPRLAGFGG"
     gene            15837..17276
                     /locus_tag="VF226_07920"
     CDS             15837..17276
                     /locus_tag="VF226_07920"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7073951.1"
                     /translation="MYDYGTFANGKWQSDGQSSFATWNPASPAQQAGRYRHTAPSSID
                     TIVSDARAAQEQWGRRPPVERARIVGEFLARIEAKAEPIAVAITREQGKPIGEARGEL
                     AKSLREARFMVSQGAQADGSVVNSQRRGVRNLVIRRPRGVIAAITPWNFPVLTPMRKI
                     VPALVYGNAIVLKPSEFTPAAVCLIAEASANVLPDGLLQIVLGGADVGRALAESRGID
                     GLTFTGSVEVGRSVYQLAARNLAEVSLEMGGKNAAIINDTDDLDRCLDQITGAAYQCA
                     GQRCTAISRVIVQERLASDVIAGLVRRAGAFRLGDGMDPSTTMGPLIHKAHLNRVDSL
                     VRQGIEEGARLVVGGAPAVVSGLEDGFFYQPTVLADVRPEMVVAREEVFGPVISVIAY
                     EEFEEALRILNGTEYGLTSALFSNDNRCIQRFLDVSAHGMMHVNHGTIPDDHMPFGGI
                     RNSGVGAYSVGRSAGNFYTTEHSAYLQYE"
     gene            18327..>19289
                     /locus_tag="VF226_07925"
     CDS             18327..>19289
                     /locus_tag="VF226_07925"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HEX7073952.1"
                     /translation="MVGNPCDAATGNKYQADADFMDTEVISGFLRHYNTQFDNDIGLG
                     RGWTASFGKRLELYGTRVQIRQGTGRGEPFNCPDTTGACTGDPDSEMQLSKDASGYTL
                     SYPAGVSEHYDTAGRLVSETDPRGRITTYGYTSGKLTSVTGPFGHTLTFAWNGGHIAS
                     VTDPAGEIYAYTYDAKDNLTRVDYPDGSAKIYHYENPSFPNHLTGISYEEPAGAVTRF
                     ATYAYDANGKAILTEHAGGMEAYDLVYDSDTQTTVTDATGTAEVMTFAANLGLKNLVS
                     KVNPADGKSLIQTFDANNNLTCKKDEEGRVTTYTYNGTNQKLSMT"
BASE COUNT         3491 a         5857 c         6199 g         3742 t
ORIGIN      
        1 acagcggcaa caacgcgagc gacacggatg atggcgaact gttctatgag ccgggcggca
       61 acgagcacaa tgacccggat atcggaactc tcgttgccac atcggacact gcgttcgttt
      121 tcggcctgga ggggcacgat accctgatcg gcaacaacat cgccggcttg acgctcgacg
      181 gtggggtcgg gcacgacgtt ctggacgatg gggatcagat cgaggccggc gcgaagctct
      241 acggccgagc gggcaacgac attcttttcg gcaggggcgg cgacgactac atcgacggag
      301 gagacgacag cgacttcgcg actggcgatg ccggcgacga tttgatcttc ggcggtgccg
      361 gtgccgattg gctcgatggc ggtacaggca aggacatcgc cacgggcgac gccggagaag
      421 acaaattatt tggcggtgcc ggccacgaca cgttagtggg cggtgacgaa gcagatcgtt
      481 tatacggcga taccgacttc ggtcagatct tcctcgtgcg ctatcagggg gatattggcg
      541 tgcatcgcag cgacgatccg aacgtcgctg ggctgtttcc gctgttccgt gacgcgccgc
      601 tcgccgaggc gggcgacgac tacctggagg gcggcggggg caacgacgag cttttcggcg
      661 gcgcgggcca cgatacgctg ctgggtggcg ccgatgacga tgtactgcaa ggtgaaggcg
      721 gaaatgatcg gctgatcgcc ggcagtggag acgacacgct gtggggcgat gccgatccgg
      781 cgacggcaaa ggcggacggc gaaacagcga ttacggcggg ctactatgag tacttcaccc
      841 gtgcccgcga cgtcggcgca gacggcgacg actatctgga cggcgggtcg ggcgacgacc
      901 gacttcatgg tgggcttggc gacgatactt acgtcttcgg ctggggatac ggtgaggata
      961 tggttttcga ggcgggcgga tacgacgctc tgctcttgga gagcaggatc cgacccgatg
     1021 atctggcctt cgaaaaggtc ggcgcggccc tgatcgtcgc cgtttcgggt gacgatcggg
     1081 cgattcttac gaactggttc ggcgcggcgt cggtcgtcga gtcctcccgg atcgaggcga
     1141 ttcgtttcgg cggcgggacg ttctgggata cggcggaaat agaacgccgt tcgggcctct
     1201 acaacctgtc cggggaaggg caggtcggcg acacaacgac gaacggcacc gactactatg
     1261 cgagtttcgg cttggacgtc gacgacggct ttgacaccgt aattgaggat gccggcggcg
     1321 aagatacgct tcacttcgac gatgtgctgt tcgcgcagac cccccttggt ccactctata
     1381 ttcgaccgat tctggaggaa gcccttcggg acgagaacga tctggtcctg cgcgtctttc
     1441 tcgattccga catcgagcgc cctgacgtcc gcggcacggt tcgcatcagg ggccacttcg
     1501 ccgaagggca agtcgaaagg atcagtcttg ccgggggccg cgtactggtc gcccccgagt
     1561 tcgcgctcgc ccgacccggc acctatttgc agcgtgatca cgatgcgatc ctgtacgaaa
     1621 cgttcgatcc ccttgcttcc tacccacacg tcgaggcctt ttaccacgtg ggcgacgacg
     1681 gggatgactt cctgcagctc tctccaggaa cgtccgccga cgtctatcac tactacggca
     1741 acgggggcga cgacgtcctt acgggtagcg caggaagcga cgtgctggtt ggcggcgcgg
     1801 gcgccgacag gctcgaagga tcctacggca gcgacgtcta ccacatcgat ccaggcgagc
     1861 aggacgtcgt actcgacgac gagcggagct atgcgggatc ctcaacggac ggcctcgtcc
     1921 tgccggtcgg cctgacgctc tccgaaatcg cttttgggcg cgaaggtgag gacctgttca
     1981 taggcagcac gcgagttgag cgctatttcg agcgcgacct gtcgcatgcc ggtggctccg
     2041 attatccgtt cctggtcgaa attctgcagg tgggcgaact tcacgtcgat ttgcccgagc
     2101 atctcatcga tcttggcctg tttggtacat attcgggcac cgatgacgcg gaaacactga
     2161 tcggggaccg gtacgacaat gtcctgcaag gcgctggtgg ggacgatgtc gtctacggat
     2221 ggcggggggc cgataggatt ctgggcgggg agggtgatga cgaactttat ggcgaacaag
     2281 acccggccct tctcatcttc cgtcacacca acgaccacat aagtggcggt tcgggaaatg
     2341 actatctcca aggggactgg ggttcggaca ttctgctcgg tgacgaagac aatgacgtcg
     2401 tgtacgggga cgaaggcttg gccgagcttc cggactgggc aatcgggaat atcttcatac
     2461 cgtacggccg agacactctg gcgggaagtt cgggcgatga cttcctctac ggagagcagg
     2521 atggtgatac gtaccgcttc gggataggag atggtgttga cgttatccag gactggggct
     2581 acgaccgctg gaacaatgat cacaccttgg gcggcgagac tcttctcgac gttcaccggg
     2641 caatcataag gctggactat ttcgaagggg ccacctaccg acccccgatt ttcgtgccgt
     2701 tccgggaaca caggctcgaa tgggccgtgc ccgatgatct tctggtcgag gtggaggcgc
     2761 tcgaagacgg cctcctcgcg cctgaggcag ctcgagtcac gctcgagcgg ctgcgcgagt
     2821 ggtacatcga tgatcagctg gatgtggtcg acttcggcgc aggcatcgcg ccggaaaacc
     2881 tcgccgtcga ggtgctgccg ggcgagctgc tgatcgccat cggaggcaat gattccctgt
     2941 cgattcggga tcccgcgggc gggagcaact tcgggatcga gcgcttccga tttgcggacg
     3001 ggtcggagct gaccctcgcc gatctcatgc tgctggacga aagtatcgac cacgagatcc
     3061 aggcagaggg tacgttcggc gatgacctcc tcatcggcgg cgacagtagc gacttcctgg
     3121 atgggcgggg tggtaacgac actttgcgcg gtcgtgccgg ggatgaccgg ctgctgggcg
     3181 gtagcacgga tacgaacacg ttcgggctgg tacccaatgc cgacatactc gatggaggag
     3241 caggcaacga ttacatggat gggggtgacg gcgcggatac gtacatcata cgcgcgaatg
     3301 agggcatcga tatcgtcgag gacggtggcg atgagttcga cacggcgata atcgaaggcg
     3361 ctatccagga actggtcgat ttcgagggcg agacctattc gaacgcgtat tgggccgagg
     3421 agctcgacga atgggcggca ttttcgatct cgcaggagct gggccgcgcg gtgctcgcgc
     3481 tgcagcacgg cgtactgcct tcggttgcac tgcagacact gatcgatctg cgcgcttggt
     3541 tcttaggcct gcatgccgat gcgatcgagt tcggcgaggg tatcacgcct gaaaatatca
     3601 ccttccactg gaacgacagc ggcgaagtct ttgtcggtac ttcccgctcc catggcgtcc
     3661 gggcccaggg catcgagtcg ctccggttcg ccgacggcca ggaagtcgcc ttggacgagc
     3721 tgcgcgattc gtccaatcgg atatccggcg atgtcgtgac tacgcagggt tccgatgcgc
     3781 ccgacgtact ggccgggtac ttcatcgacg atctgatcgc gggcaacgga ggagacgaca
     3841 tccttgtcgg atacagcggc gacgatcggc tgagcggcgg cgatggcgac gatattcttg
     3901 tcggtggccg gagcacgtca ggcaactggc tggaaggagg tgccggcaat gacgtttacc
     3961 tgttcaaccg ggccgatttt gccatcggcg agattctcga taccggcaac cccggggacc
     4021 tgccggattc ggtcgtcgag cgcgttggcg atctgaaccc cgacccgtcc gcgagcgata
     4081 tcgacacttt ttccttcggc tcgagcatct cgccccagga tatccgcgcc tatgcccgga
     4141 tgtatgaaaa ccccggaggc tcggtcgccc ttctggcttt gaccctcgac ggcgcggagc
     4201 aggggatctt cgtcgtttgg ggattggacg ccgggcaggg cgtcgcccac attggcgcca
     4261 ttgaacgcgc gcagttcatc ggtcagggcg cgcataaagt gttcgatcta gtcggaatag
     4321 tgcaaacacg cttcgatgag ctcctggcag cgtcttcgac cgacccgatc ccgctgttca
     4381 cgccggaagt ccttgcctcc tttgaaatct ccgattccgt accgctggcg ggcgggtctc
     4441 acgccgtcaa ctacgccctg accggcgacc cctttatcga accgccaaac gaggcgcccg
     4501 cagtcgcgaa gcctctgccc gatcaggcgg tccatcaggg tgatgaggtc cgattcgtga
     4561 tagcgccgga tgcttttgcc gatccggagt cccaatcgct tgtctacgcc gccatgttga
     4621 gcgacggctc gccgctacct gcatggcttg cgttcgactc ggaaacgcga actttcgaag
     4681 gcactccgga aaacaacgac gttggtacgt acctgattcg cgtgctggcg agcgacacgc
     4741 aaggattggc tgcctttgac gagtttctgc tggtcgtgca cgatgtaaat gacgcgcccg
     4801 tagcgtcaag cactctcccc gatcaaactg cttcggaaga tcacctcgtc agcttcggcc
     4861 ttccggagca agcattcttc gacgttgacg tcattcatgg cgacggtctg atgtactcgg
     4921 ccgccctggc cgacggcacg gcgttgcccc cctggctcgc attcgatccg ctgacgcgaa
     4981 cattcaccgg aaagccggcg aatgccgatg tgggggtgat cgagatccgg gccaccgcca
     5041 ccgatgcggc gggcctttct gcgtcgagcg acttcacact gaccattgcg aacgtgaacg
     5101 acgctccgct cgctgtcggc tcggtagacg atcaggtggc tctggaagac acaccctttg
     5161 gtctatttgt tcccgagtcc acttttgcag acgatgacct ggttcatggc gacgagctcg
     5221 cctataccgc acaactcgcc gacggatcgg cgctgcccgg gtggatgaca ttcgatgccg
     5281 cgacggcgac tctcagcgga acgcccgaga acagtcatgt aggcatctac gagatcgcgg
     5341 tgactgcggt cgatcgggca ggcgcgagcg ctgaaaccgt gttccagatc gaggtcgtta
     5401 acgtcaacga tgcacctgtg acgcaggctg gggtcgacga tcaggaggcc gtcgaggacg
     5461 ccgcattcgt tttccatctt ccgtcggccg cctttgagga tgtcgatgtc gtacatggcg
     5521 actccctccg ctttgatgca gtgctcaggg acggaacggc cttgcccgca tggctcgggt
     5581 tcgatccagc gacgcagatg ttctttggaa cgcccgcgaa cggcgatgtc gggcgaatcg
     5641 agatcagggt gacagcgacg gataccggcg gcctttcatc gtccgacgct ttcaccctga
     5701 cggtcgccaa tacgaacgat gcaccgttta ttgtctcggc cctcggcgac cggcatgcga
     5761 cccaggatgt accggtcgga ctgccgctgg cccaggcgtt tggtgacatc gacatgatcc
     5821 atggcgacgc gcttacgttc gctgcgacgc tggcggaagg cgggtcgctg cccgagtggc
     5881 tcgatataaa tccgcatacg ggcgtgctca gcggagtccc cggtaacgac gcggtcggac
     5941 aactctcgat cgaggtgacg gcgaccgacg cggcaggggc ggcggtatca gcgttgctgg
     6001 ggttgacggt acacaatgtc aatgactcac ccgtcgttgc gctacccgtt gccgaccaga
     6061 gcgtcaacga gtacgaggtt gtccgctacc agctctcgcc gcaagtcttc gccgatccgg
     6121 atgcaatgca cggcgacgtg ctcacctatt cggcaatgct cggggacggc tcgccgttgc
     6181 ccggctggct cagcttcgat ccggtcgggc gcactctaat ggggttgcca tcgttcagtg
     6241 agcgcggcga ccatcagatt cgcgttagcg caaccgatgc ggccggagca agcgcgagcg
     6301 tgacctttac gctgagcgtt cacggggtgg ggaacgccat atcgggtggc ccgggcaccg
     6361 atatcctgaa cggaaccggc ggcaacgacg tcattttcgc cggcggcggt ttcgacctgt
     6421 tgtccggcaa cggcggcgac gacattctgg acgcagggga cggcaacgat ctgctcttcg
     6481 gaggcagcgg cgatgatcgt ctcctcgggg gagcgggcga cgatctcctt tacggcggga
     6541 acggtaacaa tcggctccac ggcgggtccg ggaacgacct gctcgccggc ggtgcggatg
     6601 ccgatgttta tgttttcggg cgcggcgacg gcgaagatac cgtgctagct ctcggcagtc
     6661 cccgatctgc gtccgatgcg gtccacgtcg gtgccaaccc gcttgatatc ctgttctcca
     6721 cagcccgggc gagcctgcag gtggatctcc atggcagcga tgaccgcttg agcgttacgg
     6781 catggcgcgc gccggcgaac cagatcgaaa ccgtcgtggc acaggacggt tcgaccattt
     6841 atgccgctca aatcgagcag ctcatccagg cgatgaatca attctccgcc gacaacggcg
     6901 gcatcagctg ggagcaggcg gtcgccgagc gcccgcagga cgttgaggcc gtgctcgcgg
     6961 cctactggca gccggcggcc gcctaggggc gcaaccaggc gttgacggcg agcacgtcgt
     7021 cctcggccag cacgccggcc gcgtacttga gttgcagcag gttggaaaga tacgtgtgct
     7081 gcgcctcggc gaggtcgcgc cttgcctggt agagctcacg ggtggcgtcg agaacgtcga
     7141 gcgtcgtgta aagcccgaag ccgcgcccct ccgttttcgc ttgcagggca gattctgcgg
     7201 cattaattgc aagttccagc gcgtcgattc gggccaggct cgccatcacg ccctggaatg
     7261 cggctcgtgt cgatcgttcg acggcgcggc gtcgtgcctc cagttcttcg cgggccgcgc
     7321 catagcggtg agcggcctct tcggtgcgag ccgataccgc acctccctgg tacagcggaa
     7381 tcgtgagctg aaccccaatc agcgtgtcat cggcccgtat acccggcccg gggatgctcg
     7441 cgtccgcatc gtgacgcgag tgggtgccta ccatctcgag ggtcggaaaa tgccctgcgc
     7501 ggttacggtc gatctcgagc cgcgccacct cgagcgcttc tctggcagca agtacttcgg
     7561 aattctgctg caatgccgtc tcgatccatg cgctgagttc cgccggctcg ggcgcctgga
     7621 acgcagtgtc gtcacccagg ccctggagat tgccgggcag tcgatccgat atctctcgca
     7681 gcgcctcgcg gctgtcggcc agttcgttgg ttgcctcgat gagctgcgcc gcgctgacct
     7741 ggaaacgcgc gcgcgcttcg tgcacttcga tgatcgtgcc ggaaccggcg cgccgccggc
     7801 cctcggcggc ctcgaggttg cgcgccagcg cctgcctttc ggcttcggcg agcgcgacgg
     7861 tctcctgcgc cagcagcacc ctgaagtacg cttcggccac gcgcaggatc agattctgac
     7921 gcgctgcgcg gtactccagc gctgcgcggc ggacctcggc gcttgcctga tcgagccgcg
     7981 tccacagcgc ccggtcgtag aggacctggc tcagattcac ttcatagctg tcgctgtagt
     8041 agctcgcgcg gccggcgggc cgcgagaaaa taaaggcatc ggtgctgacc tcctcagcat
     8101 tccgatttcg cgcgagtgtg gcgttgactt ggggcagcag cccggcgcgc gcctgaggta
     8161 ccgcttgctg cacggcctga tagcgggcaa aggcggcgcg cacctggggg tcgttctcgc
     8221 tcgcgagacg gaagatggac aagagatctt ccgcccggag cgccgatggc agtgccatca
     8281 ggcagccgag cgcaagggca gaaaaaaggg tggctccgtg cttccggcta tcgtggccgc
     8341 cgacgtccct gataagcgac ttgcccatgg cacattatga tcctgaaacg agtaggggaa
     8401 tcaaccgctg cctaatcggg acgcaatatt tgcgcggagc aaaggggcct cgaccgacgt
     8461 tcgttgtggt cttggctaca tcccattcct tttctgtgcc cggtggccct gcaacggttc
     8521 gggctcggtt agcgcagcgc tgggtactgg ttctaccagt tggtttccgc aggctggcta
     8581 tactcaaacc ggtccgcccg attcgactgt tcatgcaagt aacgcccgac cggccccgcc
     8641 aacggcgtta tcaagtcgag cgccgccaag gaaacggcag tcatgctcct tttttgccct
     8701 tgtttggaac ggcatgaccg gggcgctcgt ctggaacttc cgggccgagc tgagggcgga
     8761 cacggtcaag gcgccctggg tcggcgtgtt cgtcctggca ggtctgtggc tcctctatct
     8821 ggccggcaag tcctggctgc agtggcgccg cttcggcacc ctgcctctca ccctggatcc
     8881 gtaccgggta acggtcggcg gcgacctggg cgggacgatc gagatcccgg ggctgcgcgc
     8941 gacgtacgac acgccgttcg aagtcacgct gagctgcgta cgcgtcacca tccggcgctc
     9001 gggcaagagc cgaaagagga acgagcgcgt cgagtggcgc gagcgcggga cggttacggg
     9061 cgcgccgggg gtgcgcggca cgcggctgca gctccggttc gccgtgcccg gtcacctgcc
     9121 ggcgaccacg ccaccctcgc gcgattacca taagtgggtt tgcacgtgca cgccgcgctt
     9181 gccggggtcg atctggatca gacgttcgag gtgccggtgg agcaggccgc cgtggtcctg
     9241 caacgatcgg ctcgcggtat cgagtacacg cgcgccgagg agcgcgcgcc gatggcggcc
     9301 ggggacggcg cggcagcggc gggcaccggc gtggagatcg gccacggcgc tcaggggctg
     9361 cgacttttct atccgagcgc ccgccaacgc ggcatgggca tcgggttcga ttctcggtct
     9421 cgtgttcggc ggcgtcccct tgtttttcgt ctcccagata caaagcgcga ccgcgggcga
     9481 cgcgtttcgt ttcggtcggg atcatgagct tcgtgggcgc gatgttcgcc ctgttcgcgc
     9541 tccggctcat cggatctact tgatccgtaa cagtctggag gtgatcgtct cgacgcacag
     9601 cgtcgatacg gtccgacgtc tctacggcgt ccccgtgcat cggcgccacg cactgcgcga
     9661 gtccattacg ggcctgcaat cgaagatctt cgggccgagc ggccagggta cgaaggccaa
     9721 ggtgagctac accatcaagg ccgtgctgag cgacggcagg accctggctg tcggcgacgg
     9781 catccgcggc gtcaccgaag cgaagcgcgt gctcgcgcga ctgcgcgagc acctgggtct
     9841 tccggcgaga aagcagtcct gtgtaaggtt gccgcgacaa ggcgggcagc gatcggcgcc
     9901 gaccgatcgg gcccgatagc acagagatac gaacgactcg aagggcggtg acctcctcgt
     9961 catggaccga gggggcaggc tggtcagcgt gtcgcgggcg aaaggttttg ttcgatgcgc
    10021 acataaacgc ccgagaactg ggcgcgagag tagagtaccg tgttgcccct gctgtccgcg
    10081 atgtggcgct cgatcttcac aacgggctct cccagcgtga tgttcagcgc ctccgatgtg
    10141 acgtcgtcgg atatggaaaa cgtaacaacc tgcctgacct cgtgcaccag ccccctgtga
    10201 tcgtcgttga gcacgaggat catcggttgg ctcatgaatt tcttttcatt cgagtagaac
    10261 agatccttgt cgatataaat atcgatcaag cagtacgggg cgctgcctat tgaatgcaga
    10321 cggcggacgt gacaaggaac gaaaccctcg ctcaacggaa agtgtggaga gggcgtaatt
    10381 gcggcaactt gccgcgggcc ggccagtacc gaaagcttga cggggtgcag ggacgccacc
    10441 agttgctccc agctggcggg aagcacgtgc tcctggcgcg cgggcagatg ctcggtgacg
    10501 aaggtgccgc ggcctcgatg acgtcgaatc aagccttcct gttcgagcac ctctaacgcc
    10561 tgtcggatcg ttacgcgcgc cagaccgaac tctgcctgca aagcctcgag caccggaact
    10621 tgctgtcccg gtgaccacat gccgttctcg atccggaggc gaaactgcgc ggccacctga
    10681 aggtagagcg ggatcttgct acgcgcgagg agagtgcggt tcgtcatgcg gtgagcaaat
    10741 caaatgtcac tggatcgtat ggccgagagc agacaacacc gtcattgctt catggattgc
    10801 gcgagcgcgc gtgccgagtt ggccagaaac gccaggtcgc ttcccacggc caccatggtg
    10861 tagccgaact cgaggcatcg ccgtgcaacg tccgcccggt tgaacaaagt gccgcaggga
    10921 acgccgattc gcgcgcagcg ggaagcggct tccttcatga tcgacagtac gtcctcgtgt
    10981 gtcggctgac cgagaagccc catggcggcc gacaggtcgc ccggaccgat aaagaccgcg
    11041 tcaatccccg gtaccgtggc aatctcctcg agccgatcga tcgcccccgg gctttcgagt
    11101 tgcgcaacga tgcagatgtg gtcgttggcc acgtgcgcat agtcttccac ttgcaggtag
    11161 cggctggcgc gatgcatgag cgcgaagccg cgcgtaccgg ccggcggata tcgagtcgcc
    11221 gccacgatgg ctttcgcctg gtccgccgtt tcaaccatcg gcacgagaag ggtctgtgcc
    11281 ccgatgtcga gaagtctctt gatgtaagtc gcgtcgtgtc cgggagtccg tacgacgggc
    11341 aagctgggcg tgcccgcgat cgcccgcagt gagtcgagta tatcggccgg tgagtacggg
    11401 ccgtgttccc cgtctatggt gaggaaatcg aaaccggtcc accccatcgt ttccacgatg
    11461 ctggccgatc cggccatgca ccatgagccg atgagcgtct cgcccgccag cagtcgcttt
    11521 ttgaactcat tcgaaatcga caccggaacc gcctccgctt ttccttaaag gctccgcgtt
    11581 cgtccgggac gcgagcggtg ccccacagac accatccccg cagattcgac cgcagattga
    11641 caagttctat agtaccatct tatgctcttt agcctgcaac ccttcgtcgg caagcaacgc
    11701 cgcggggcga gaagcacgct cccagcgctt tccgtgggct gggagtgggc acaggtacag
    11761 cggaccggct gtgggtggtg agcgatgcga ctaggtagct tccagagaaa taccggcgag
    11821 caagggccgc gtgtcggtct cttcctcgac gacggtgtcg tcgacgtagg ccgtgcaaat
    11881 cctgacattc cctccgatgt gcgcgggatt ctcgctggcg gcgagcggca gctggcggcg
    11941 ctcgaggcga tcctcagtca gcaccagaaa ggtgccctgc cgcaggacgc attcgttccc
    12001 ttcgatcagt tgcgcttccg gccgcctgtc cggccgggaa agatcatctg cgtggggggc
    12061 aactacgagg actacaggcg gattctgaag ctgcccgatt tgccggtacc ggcgttcttc
    12121 ctcaagtcgc cggatgccgt catcgcccac gacgaggaag tccagatacc ggaaggctac
    12181 ggaatcttct atcaggaatg ggagttgtcg tgcgtcattg ggcggcagtg ccggcgcgtg
    12241 tccgagcaag aggcccgtga ctgtatcgcc ggttacacga tcgtcaacga cattacgggc
    12301 cacacgctcg agaccctggg gccgcgtccg tatcacatgc tgggcaagaa catggacacg
    12361 tttgctccta tcgggccctg gatcgtagcg agggactccc tggccaagga cgtctatcag
    12421 ttgcgcgcac ggcgctggcg gaataacgag ctcgtctgcg agtccagcac gcgggagatg
    12481 cgtcgttcct tcgaggaaat catcgtttat gtttccaact tcatgactct ctatcccggt
    12541 gacatcgtta ccggcgcatc cccgcctgcg ggtccgatcg agcccggcga cgtcatcgaa
    12601 gtggagttcg aagccgtcgg caggttgcgc aacacggtag gccgtgccga cgcggatccg
    12661 atctacggag agagaatcgg catcgcctcc ggcaaatgat gccccttgtg ctcgactccg
    12721 acagggatgc ggagcggtcg aaactcaacc ctgttttgca tccggaagaa gtggagcata
    12781 gtcatgagaa agttccttct agtggccgtt tcactcgtca ttctcgcggg ggccggttct
    12841 gccaccgccg ataccgttct gcgcttcgct cacgtgaact cgccgaattc cccggcgggc
    12901 aaggcggcgg aatacttcca ggaacgggtt cagctccata cggagggccg ggtacgcgtg
    12961 cagttgttcc cggccagtca gctcggaaac aaccgccaga tgttctcgag cctgcgcggc
    13021 ggcagcatcg acctgtcact gactccttat ccgatgctcg cggatatcgt gccggagctg
    13081 tcggtctacg tggccggcta catgttcgaa agcttcgagg agcagcagcg aatcctgcag
    13141 catccggagc tcggcgcatc gtgggccgac cagcttcgtg aaaagggggg cttgcgggtc
    13201 ctttgcagtt tctactacgg ctcgcgcaat ctgacgacca cgaacaagcc cgtgcgcact
    13261 cgcaaggaca tgcagggtct caagatccgt gccgtgcccc agcccgcctc gatgtccgtc
    13321 attcgcggct tgggcgccac tccgaccccc gtgccgctgg cggaagtctt tcagggtttg
    13381 cgtcagggag tcatcgacgg gcaggagaac ccgctgcctg tcatctacaa ccagaaattc
    13441 ttcgaggttc aggactacct gatgctcacc aatcatcagc tggtcccgct gccgtacgtc
    13501 atgaatgaag ccaaatggaa gagcctcggg cgcgatcagc aggcggttca gcgcgcagcg
    13561 gatgaagcct gcgcgcgtgg gaccgagctt accgtgcaac aggaacggga gctgatcgaa
    13621 accttcaagg cgcgcgggat gaacgtgatc acggcgcagg acggcctcga catcgagggc
    13681 ttccgcgcga gcgtgcgcga agaggttcaa aagacgttcg acggcgagat ctggccggcc
    13741 gggacctggg cgaagatcca gtccgccctc ggccgatagg cctcgcccgg ccaagggggt
    13801 ctgcgggcag gaagaggaag cgggccgtag gtgtcgtggg ggatcgcatg gggcctgaag
    13861 cggaagtcga gaaaagcgac gaggaaagac tggccgagga aggcggcgta cttcgcaatc
    13921 tcgagatcaa tatcgcggcg ctcgcgctgg tcgcgttcat cgtgctcgct tccgcgcagg
    13981 tcatttcgcg atacggcctc aataggccaa taccctggat cgaggaagta tcggcaatac
    14041 tgctgatctg gatgaccttc atcggcgccg cctggctggt acgaaaagat cggcatatcc
    14101 gcgtggagtt gctcgaggag tgggtacatc cgcgggtgtg tgcggtcatt tacaccgtct
    14161 acgacatcat ctcgattgcc ttcctgatcc tgctcgccat cggcggcttt cagctggtcg
    14221 gagcgatgga cttcgagcgt acgccggcgc tgcaaatacc gtacaggtac atcgtcagtg
    14281 tggtaccggc tacggcattg ctgatggccg cctacttcgt ggtgatcatt taccggcgta
    14341 tcgccggaca gaagaagaac taggacatgg accctctcct gatcggcctc gtagtcgtca
    14401 gcctggttct cgttcttggc ggctaccagg tcgctttcgc gctcggcgcg tgctcgttgg
    14461 tgtacctgat catcaacgat tttccgctga cgctggcggc acaggaactt gcgctgtcgt
    14521 tggacaagtt cacgttcctg gccatcccct tgttcatgct ggcgggcatg atcatgaacg
    14581 ccggcggcat cacggagcgc attttcacgt tcgcgcgcct ggccgtcggg cgcattccgg
    14641 gcggattggg tcacgtgaac gtgttggcaa gcctcgtctt tgccggcatg tccgggtcgg
    14701 tcctcgccga tgtggccggc ctgggcagca tggaaatcca ggccatgcgc aagagcgggt
    14761 accgaagcga tttctcgatc ggtatcactc tggcctcgtc cgcaatcgga ccgatcctgc
    14821 cgccgagcgt gcccatggtc ctgttcggcg tgatcgccga cgtgtcggtg acgggactgt
    14881 tccttggcgg cgtcgtcccc gccttcgtca ttgccggcat gctgatgatc tacgtctatt
    14941 ttgtcggcaa gcgcgagggc tactttacga ccgcctggga gggctggcgg ccgcttgccg
    15001 tggcgttcgt ggtggctttt cccgtgctca tgacgccggt gatcatcgtc ggcgggatga
    15061 gcctcggcat cttcagccct accgaggcgg ccaccgctac cgtgctctat gcgttcttcc
    15121 ttgcagcggt ggtctatcgg gacttgccgt ggaagcgcgt tcccgcgatc ttcaggaccg
    15181 tcgcgatttc gaccacgaag ctgctgtttg tgattacgtg cgccctgctg ttcggttggg
    15241 tgctcaccgt cggtcaagta ccgcaaacgc tcgcgaacgc cctgggcgag acgtttgacc
    15301 aagcctgggt gtttcttctg gtagtcaaca tcaccctgct ggttctcggt gcgatcatcg
    15361 agaacgccat tctgctcatg gtcttgggac cgatgctcat tccaatcggc gagtcgatat
    15421 acgggctcga tcccattcat ctgggcgtag tcatggtgtt caacatcatg atcggacagt
    15481 acaccccgcc catgggactc tcgttgttca tcgttcgcga tatcaccggc caatcgctgg
    15541 cgcaggtttc ttgggccgtg tttccgttcc tgctccccct gatcgcggcg ttgctgctcg
    15601 ttacctacgt gccgggcctc gttctgtggc tgccgcggct cgcgggcttc ggagggtagt
    15661 cggccggggt acgccctctt tccaagccac gaggtccgat gccgatatct ggctcggtgg
    15721 ttcgggcgcg acgtcgcgcc catctgtggg tcaggagccg gccgttgcat gaggtggaaa
    15781 cgcctgcgat ccggtaggga cggttgctcg cgctcggtac ggaaaggagg ttcactatgt
    15841 atgactacgg caccttcgcc aatggaaaat ggcaaagtga tggacaatcg agcttcgcga
    15901 cctggaatcc cgcttctccc gcccaacagg caggccgcta tcgccacacg gcgccttctt
    15961 ccatcgacac catcgtctcg gatgctcgag cagcgcaaga gcaatggggc cgacgtccgc
    16021 cggtcgagcg ggctcgcatc gtcggtgagt tcctggcccg gatagaggcg aaggcggagc
    16081 cgattgccgt cgccatcacc cgcgagcagg ggaagcccat cggcgaagcg cgtggcgagc
    16141 tcgcgaagtc gttgcgcgag gctcgcttca tggtctccca gggcgcgcag gccgacggca
    16201 gtgtcgtcaa ctcgcagcgc cgcggggtac gcaacctggt tattcggcga ccccgaggag
    16261 tgatcgccgc gatcacgccg tggaactttc cggtgcttac gccgatgcgc aagatcgtgc
    16321 cggcgctcgt ttacggcaac gcgatcgtgc tgaagccgtc ggagttcact cccgccgctg
    16381 tctgcctgat cgcggaggcc agcgcgaacg tgctgcccga tggtcttttg cagatcgtgc
    16441 tcggtggtgc ggatgtcggc cgcgctctgg ccgagtcgcg aggtatcgac ggtttgacct
    16501 tcaccgggtc cgttgaagtg ggccgcagcg tctatcagct cgcggcacgg aacctggccg
    16561 aagtgtcgct cgagatgggc gggaagaacg cagcgatcat aaacgatacc gacgatctcg
    16621 accggtgcct ggaccagata accggcgctg cctatcagtg cgcgggccag cggtgcacgg
    16681 cgatcagccg cgtcatcgtg caggagcggc tcgcatccga tgtgattgcg ggcctggttc
    16741 gccgtgccgg cgcgttccgc ctcggagacg gcatggaccc gagtacgacg atgggaccgc
    16801 ttatccacaa ggcgcacttg aaccgggtcg actccctggt aaggcaaggc atcgaggagg
    16861 gcgcgcgcct cgttgtcggc ggcgcgcccg ccgtggtatc tggactggaa gacggcttct
    16921 tctaccagcc gacggtcctg gccgacgtaa gacccgaaat ggtcgtcgcg cgagaggaag
    16981 tgtttggtcc ggtcatatcc gtcatcgctt acgaagagtt tgaagaggcg ctgcgcattc
    17041 tcaacggcac ggaatacgga ctgacgtcgg cgctcttttc caacgacaat cgctgcatcc
    17101 agcgctttct ggatgtgagc gcccacggga tgatgcatgt caatcacggg accattccgg
    17161 acgaccatat gccttttggc ggcatccgga actccggggt gggcgcctac tccgtaggtc
    17221 gctcggcggg aaatttctat accacggagc actcggcgta cctccagtac gaatgagagc
    17281 agcaaaagct gcgggcggct tgcccctgac tgcctgccgc gagggcctga gcccgcccga
    17341 cctcggtcgt gatgtcaccg gccacggcat cgcttcgacg ccgctggcat tccagatctc
    17401 ggccgcggtt gcttccagtg cctccgcccc gcgccgtaat caccagcttt gcgccttccg
    17461 ccgtcagcgc tgcgcacagc ccttcccgaa cctcttggag tcgtcgcgtg ttcatcgctg
    17521 gcgcgattag cgaccctgct gcgcttagag atcgaacaaa agattctcgt gaggcctccg
    17581 ctcctccttg ataggcgatt tcagactggt cctaccagct gcccaacggc gacccaataa
    17641 ccctttgagt tttccctaag gggcattgac ggtgcgccgc ttctccctta gcttcgggga
    17701 caacaagaac cagacaggga ggcgcaatgg atgttgcggt ctggcttttt gacaggcgta
    17761 agcctgagag ttgtggtgct gtccttcaca acttcacgat cacctgtttt ctcacccgtt
    17821 taggtccagc catacccagt aatttggcgg tcgagccctg ctgtttgtct ttccgtccgt
    17881 ggcgttaagg gcggattatg cgggggtgtg gcgaaagatc gatagaacaa tagggagggc
    17941 gcatgcgggc tcgctcatcg attcctatcc tcttgcttgc cgtgagttgc gcaactagtg
    18001 ccgcaacggt tatccggggg cactactatc cgaggcaacg cgccgctgac acctgtaacg
    18061 ccgagtatag gaatccgtgg ggaacttgcg ggatcgcgga gcagggcaaa cggtatccgc
    18121 agtgtgcacc tagctactac ggcgatatgg cgtggtactt cgggagcaac ggcacatggt
    18181 acatctatcg gggatgcggg aatggatgtg ccgaaggata tgagccgaat ccagacacgc
    18241 acaagtgcga acccaaggca ccaccactgg ccgatcgcca agacgagaag aatttaggtc
    18301 gatgcaaagc caaagcggcg accggcatgg tagggaatcc ttgtgatgca gctacgggga
    18361 acaagtacca agccgacgcc gatttcatgg ataccgaagt aatttccggc ttcttacgtc
    18421 attacaatac ccagttcgac aacgacatcg gtctggggcg cggctggact gcctcgtttg
    18481 gcaagcgtct tgagctctac gggacgcgtg tgcagatccg ccaaggaaca ggtcgcggcg
    18541 agccttttaa ctgccccgac acaacgggcg cttgcacggg cgacccggac tcggagatgc
    18601 agttaagcaa agacgcctcg gggtacacgc tttcctatcc ggcaggtgtc agcgagcact
    18661 acgacaccgc agggcgcctc gtttccgaaa ccgatccccg aggccggatc acgacctacg
    18721 gctacaccag cggcaagctc acctcggtca ccggcccctt tgggcatacc ctgacctttg
    18781 cttggaacgg cggtcacatc gcctcggtca cggatccggc cggtgagatc tacgcctata
    18841 cctacgacgc caaggacaac ctcacccgcg tcgactaccc ggacggcagc gccaagatct
    18901 accactacga gaatccttcc ttcccgaacc atctgacagg tatctcctac gaggagcccg
    18961 ccggcgccgt cacccgcttc gcgacctacg cttacgacgc gaacggcaag gccatcctga
    19021 ccgagcacgc gggcgggatg gaggcttacg acctggtcta tgactccgac actcagacca
    19081 cggtcaccga tgcgaccgga accgcggaag tgatgacctt tgcggccaac ctcggcctca
    19141 agaacctggt ctcgaaggtg aacccggccg acggcaagtc gctcatccag acgttcgacg
    19201 ccaacaacaa cctgacctgc aagaaagacg aggaaggccg ggtcaccacc tatacctaca
    19261 acggcaccaa ccagaagctc tcgatgacc
//
LOCUS       DASVDU010000124         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1740990, whole genome shotgun sequence.
ACCESSION   DASVDU010000124 DASVDU010000000
VERSION     DASVDU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740990"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..482
                     /locus_tag="VF226_07930"
     CDS             192..482
                     /locus_tag="VF226_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7073953.1"
                     /translation="MIETFGNTLAQDLFEDRHTRATRSFPAELRRAARRKLQYLHEAA
                     DLRDLRSPPGNRLERLRGALQGFHSIRINDQWRLVFRWSGGSAFEVQIVDYH"
     gene            497..766
                     /locus_tag="VF226_07935"
     CDS             497..766
                     /locus_tag="VF226_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7073954.1"
                     /translation="MAREPKNPFHPGEILLEEFLEPMGMSQATFAEKIGWTRTRVNEL
                     IRGKRGVTAEAALDLAEVLDTSPKLWMNLQASYDLDRAKKARDAA"
     gene            1116..1382
                     /locus_tag="VF226_07940"
     CDS             1116..1382
                     /locus_tag="VF226_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073955.1"
                     /translation="MGTAPPGMLVSRRDRGLEDVLNLAGVLRASIGDESDSRAMQAET
                     VLKIIERKADKALDQLERHDGQHGNLFFAYFDLMVTAEQGGDGE"
     gene            1566..1856
                     /locus_tag="VF226_07945"
     CDS             1566..1856
                     /locus_tag="VF226_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEX7073956.1"
                     /translation="MIAYLDACMEEASDDTALVAVALGNVARAYGMARLAKETGLTRE
                     GLYKALSPEGNPTFGTVLKVVKALGLKLRPEAAREAERARGSDSATCQTSDE"
     gene            1866..2009
                     /locus_tag="VF226_07950"
     CDS             1866..2009
                     /locus_tag="VF226_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073957.1"
                     /translation="MDRQHFNQLVKGVREMKRDMDGKSVRGARATELKDPDVRAFRKA
                     AGK"
BASE COUNT          515 a          708 c          774 g          407 t
ORIGIN      
        1 cgggatcgag ggtgaccgtc gtgcgcatgg aggcatcatg atgcgcaaca cacgggtgct
       61 gtcaagatgg ctcaggggta cccgaagccc ctcagccagg cccgccgccg gacctgccag
      121 cttattgacc agacgagtac ccgccatccg ccgggattga gcggattatc gagttccgat
      181 aacatcggta cgtgatagag acgttcggga acactcttgc ccaggatctg tttgaagaca
      241 gacataccag ggccacgagg tcattcccgg cagagctgcg tcgcgctgcg cggcgaaagc
      301 tgcagtacct ccatgaagcg gcggatctga gggatctcag gtcgccccca ggaaaccggc
      361 ttgaaaggct gaggggcgcc ctgcaagggt tccactcaat acggatcaac gatcaatgga
      421 ggctggtttt tcgatggagc ggcggcagcg cgtttgaagt acagatcgtg gactaccatt
      481 gacggggcaa agagccatgg ccagggagcc taaaaatccg tttcatccgg gcgagattct
      541 gctcgaagag tttctcgagc caatgggaat gagccaggcc accttcgccg aaaagatcgg
      601 ctggaccaga acgcgtgtca acgagctgat tcgggggaaa agaggtgtga ccgccgaagc
      661 ggcactcgat ctcgcggagg tcctggatac gtcgcccaag ctatggatga acctgcaagc
      721 gagctacgat ctcgaccggg caaagaaggc gagagatgcc gcatagctga cctggccgat
      781 tctgcctaga gtgcaaagct cgctcgcagt cggctaagaa ggcccagaac ctaaggcgag
      841 gccgatctgg gctgtatcga taccggcaaa gcacggtctg catttatcaa atcgagccga
      901 cggcgacctc gcgatcaata gatctcgtcg ctgatggata gcacccgcca ccgcacatcc
      961 ggcgccgagg cactcgatcc gccgcgcgca agcgcccggt tcccataaga tcgctccgcg
     1021 tcggttttcc aggacctgtc ggacatcacg tcgggaacgt acaacgcccg atcttcccgg
     1081 acctgggatg cggccgccgc ccagggagac aggcggtggg cacggccccg ccgggcatgc
     1141 tcgtttcgcg gcgcgataga gggctcgaag acgtgctgaa cctcgcgggc gtgctgcggg
     1201 cgtcgatcgg cgacgagagc gacagccggg cgatgcaggc cgaaaccgtg ctgaaaatta
     1261 tcgagcggaa ggccgacaag gcgctcgacc agctcgagcg gcacgacggc cagcacggga
     1321 acctgttctt cgcgtatttc gatttgatgg ttacggcgga acagggcggc gacggcgagt
     1381 gaggcgaacg gcgggcattc gatcgttact cgattgcccg ccacccgaca agcgtacgtc
     1441 gcggccgtcc gacgctgcca ttctccggag atgtcgccgg ctgtcggcga ggtgcggtgt
     1501 cgagctctca catccggcgg cgttgcagaa actggccgga aggagcgttt tcttcagtcg
     1561 ccgacatgat cgcttatctg gacgcgtgca tggaggaagc atccgacgat actgccttgg
     1621 tcgctgtcgc gttggggaac gtcgcgcgtg cctacgggat ggcgcggctg gcgaaggaaa
     1681 ccgggctcac gcgcgagggt ctttacaagg cgctctcgcc cgagggaaat ccgacgtttg
     1741 gcaccgtcct caaggtcgtc aaagcacttg gcttgaagct acgaccggag gctgcgcgag
     1801 aggctgagcg agcgcgagga tctgactcgg caacatgtca aacaagcgat gaatgacatc
     1861 gggtgatgga caggcaacat ttcaatcaac tcgtcaaggg tgtgcgggag atgaagcggg
     1921 acatggacgg caagtccgtg cgcggcgcac gcgccacaga gctgaaggac cccgatgttc
     1981 gagctttccg caaggcagcc ggcaaatgac ggctgatcac aactcgattc ggggatgccc
     2041 aaagccaaag ggcagtcggg atcgtcgctc acagccgctc tcaaatgacc gtttttccgc
     2101 actgaacccc gtcaaagtgc cggcaagaac ggcgccgcca atctcactga cctttgcttc
     2161 ggcacaaccg gcgaagctta cttcgccgtt gacagatgtc acgggctctg gaccccgtgt
     2221 gggactaaaa tcctccacca tgcctggagc ttcgccgcca cgtcgggatc gagggtgacc
     2281 gtcgtgcgca tggaggcatc atgatgcgca acacacgggt gctgtcaaga tggctcaggg
     2341 gtacccgaag cccctcagcc aggcccgccg ccggacctgc cagcttattg accagacgag
     2401 tacc
//
LOCUS       DASVDU010000125        12728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_995183, whole genome shotgun sequence.
ACCESSION   DASVDU010000125 DASVDU010000000
VERSION     DASVDU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_995183"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VF226_07955"
     CDS             complement(<1..656)
                     /locus_tag="VF226_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEX7073958.1"
                     /translation="MAQELKRNVKSVAAAYAVGKRTYYKIQGEKGLLLAVYATGRRTW
                     LVRYQTGKGERRKERTQVIGDAEHVGLAEACKRRAEIMAEAGRGGDPRSPKTFGELFR
                     AWLEGHAKKQLATWSDEEKRYRMHLAKPLEACLFAEIQRKDVREIRDNVAAKAGPIQS
                     NRVVALFNRVANWAVDEDHASFNPANRLRKIGQERRRERVLSDDELRRLWAALDEPLA
                     V"
     gene            1052..1303
                     /locus_tag="VF226_07960"
     CDS             1052..1303
                     /locus_tag="VF226_07960"
                     /inference="COORDINATES: protein motif:HMM:NF022833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEX7073959.1"
                     /translation="MRRLPRHYAPFVYGVIQAAITSAVATIIATYQMASLDLHFIIYW
                     LQCWTLSVLTMLPVVILVSPLIQRAVFSLTEAKTPGESV"
     gene            complement(1407..2150)
                     /locus_tag="VF226_07965"
     CDS             complement(1407..2150)
                     /locus_tag="VF226_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7073960.1"
                     /translation="MTGRLVGKRAFITGAGQGIGRAAALAFAREGASVVAASRTLSKM
                     EDLPGLDARIRPVELDVTNGAAVQRAIADAGEVDVLLNCAGWVHNGTILDCTEEDWAR
                     SFDQNVTSMFHTIRAVLPGMIARGRGSIINVASVASSITGVANRAAYGASKAAVIGLT
                     KAVARDVIKTGVRCNALCPGTTHSPSLEARIQAADDPEEMRRVFLGRQPMGRLGTVEE
                     MAAAAVYLASDESAFTTGTTLIADGGQTL"
     gene            2325..3608
                     /locus_tag="VF226_07970"
     CDS             2325..3608
                     /locus_tag="VF226_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073961.1"
                     /translation="MQRIVASLLLALALSVPVSAPGWRAAAQPVHISISCGALGVELK
                     LCTEAIEEWERRTGHKATIVTTPNSSTDRLALYLQMLASGADDIDVLQIDVVWAGILA
                     SHLIDLGPYVGNAPKQHFPVLIENDTVDGRLVAMPWWADVGVLYYRKDLLAKHGFTPP
                     RTWQEMTETARAILQAERAAGNRGVWGFVFQGKAYEGLTCNALEWIDSFGGGTFVNDK
                     GKITVDNPRAIAALALAASWVGDIAPPGVLNYDEEAARGVFQTGNAVFMRNWPYAWTL
                     AQGKDSPVRGKVGVVPLPKGGPDGKPSGALGGWHLAVSRYSKHPKEAADLITFLTSAE
                     QQKKRAIEASYNPTIPALYEDPEILRANPFLGELLEPLRNAAARPSRIAGPHYNRLSS
                     DVWRAAHDILAGAPAEMRVAQLAAELDRLRYRAGW"
     gene            3605..4531
                     /locus_tag="VF226_07975"
     CDS             3605..4531
                     /locus_tag="VF226_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7073962.1"
                     /translation="MIRKELSISAERKRAAWLFLAPMLAVLVVVAAWPLLRTIYFSLT
                     NATLVDMGRYDFVGLSNYVAYDNGRWYGVLTDPQWLISLRNTLVFAIVSVSLEIVLGV
                     SIAMLLNAKFPGRGMLRAAMLVPWAIPTVVSARMWGWMLNDQFGVVNDALMRLGLIAE
                     PVAWLADPTLSMISVILVDVWKTTPFVALLALAALQMVPKDVYEAARVDGIHPVKVFF
                     QVTLPLIGPALAVAVIFRLLDAMRMFDLVYVMTGTTDATMTLSVFARRELVDFQDVGY
                     GSAASTLVFFIIALLTALYVMAVRLDLNGRRR"
     gene            4528..5355
                     /locus_tag="VF226_07980"
     CDS             4528..5355
                     /locus_tag="VF226_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7073963.1"
                     /translation="MNRVLTGTGLAIACIVLAVFLLFPLYYAVLTSFKAGSELFRVDY
                     WPTDIDFSSYVRIWQEQPFARNIFNSVLVATLVVLLSLLLSLTAAYALGRVQFRGRKF
                     LLYTILGVSMFPQVAVLSGLFEVIRALGLYNNLGSLVLSYLIFTLPFTVWVLTTFMRD
                     LPLEIEEAAIMDGLSPFVIVTRIFMPLMGPAMVATGLLAFIAAWNEFLFALTFTLSNE
                     QRTVPVAIALITGASEYELPWGNIMAASVIVTLPLIALVLIFQRRLVAGLTAGAVKG"
     gene            5359..6417
                     /locus_tag="VF226_07985"
     CDS             5359..6417
                     /locus_tag="VF226_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003573220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073964.1"
                     /translation="MAGVRLEKVTKRFGRTEVIRDVDLEVADGEFLVVVGPSGCGKST
                     VLRLIAGLEEVTSGRIWIGGKDVTNLPPAKRGVSMVFQSYALYPHMDAYKNIAFGLQM
                     SGTSGETVDQRVRRVARMLQIDDLLHRKPRELSGGQRQRVAIARAIVREPQVFLFDEP
                     LSNLDAALRAQTRLELTRLHDELKATMIYVTHDQLEAMTLADRIVLLNAGRIEQAGKP
                     TELYQQPATRFVAEFIGSPKMNILPVTVEAGVVILPSGERLQAPVAHDGPADLGIRPE
                     NIALVTREEANAIRARVLHVDELGESRIIHAALDGRTTLAIRHPADRPAPSKGDEIAV
                     RLDPRHLHLFAPDGSRVQ"
     gene            6516..7610
                     /locus_tag="VF226_07990"
     CDS             6516..7610
                     /locus_tag="VF226_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073965.1"
                     /translation="MAKSLLAMVAGASVVALGLSLPIGDANAQTTFAGKRIQVIVPFA
                     PGGATDVAARFLEKYLERHLDGNPDVEVVNRAGGGSILGANWFQENAKPDGETVLFTT
                     SSTANPYVLGQKEVKYDLASMRVGYSLPFGSVTYVSAKTGIKSVKDLKNTKTELIYGG
                     IAAAASDLPTLLSFEVLDLDVRAVLGFTGRGPIRLAFDRGETNLDFQFTPVYETQVAD
                     QVASGKVIALMTGGSVGPDGKLTARDPAVPDIPTVYEVYKELNDGKEPSGVEWDAYQS
                     IGALTLGYGLTAYLHKDTPDEVIELFAKAVERINADPEFLKEGKEVIGGYKLISPKDA
                     EKPLKAALQPSEEVRSYLRKLLSEKYGVTF"
     gene            7665..9620
                     /locus_tag="VF226_07995"
     CDS             7665..9620
                     /locus_tag="VF226_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7073966.1"
                     /translation="MEHAIEALAVLFDPLRLVALFAGMLAGMICGMLPGLGGVATASI
                     LLPFVFLLDDYSGLALLLGALSVVYTSDTITSVLVGAPGSPASAPTAIEGHALARQGK
                     AAMALGVGFLASMAGGLIGAVTLAFAIPIAGPIVLALGTPELFMFAVVGLYFASSMIG
                     RDLATGVLAGCIGILLGVVGAAPAAANYRFTFGQAYLLDGLSLTIVALGLFAVAEVIG
                     MLASGGGIASGRQLLTDWGAGFTEFWRNRWLILRSSIIGVFGGFVPAVGASASTWVAY
                     GHAVRSTPDKSRFGHGEIRGIAAAEGANNSTVISDLVPTLLFSVPGGPAAAIFLGALF
                     SFGFYPGPRMVSEHPTLMYVIVWSVALASIIGAAICFAVTPFIARMTQIRFALIAAPL
                     VLIMVAGAYQATQSLGDIAVLLILGLVGWLMKQADWPRAPVLVGFVLAAPMEQYFWLT
                     TQIHGWSWLLRPGVLIIASFIVVPLFIQLTQHWRGGRAERIEESANPRSTTSAQHGTT
                     FTRCVLASLAVPVFAYALWETMQFSPSSRLLPLLAIVPGLALAIFILGRELWRLKTHV
                     EEFRFDATDEYGVILGFVVYGIGIALLGFNIATIGFLGWVLFTCAKMRPITGFIYGVV
                     LIIAINGIFHLMRVAPPDGLLMSSSLF"
     gene            complement(9657..11384)
                     /locus_tag="VF226_08000"
     CDS             complement(9657..11384)
                     /locus_tag="VF226_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7073967.1"
                     /translation="MSANEMTGGEALARMIQAHEGGPMFGMGGFQLLPFYDAARRLGL
                     AHHLINDERAAVFAADAYAKVSGRVGLADATLGPGATNLVTGLVEALNAGSPLVVLTG
                     DTHRDHSWKNMTQESRQLEILRPAVKEVIRVESVQRIPELMRRAFAVATTGRPGPVVV
                     DIPEDVCHGTHGYEESDFHADLAYQAAPALRCRPDARSLERAARMIAEAKRPILLAGG
                     GIHISGAAEALTELARAINLPVAHTMTGKGAVPCTDRLNAGLFGRYDRIANQLIDESD
                     LIIVVGCKLGEIATKRYTVPPAGKRIIHLDILAEEFGRTREPEVALWGDAREGIRDLL
                     TALSSDAAAIRERQADYAADVAKRMQAWREGVRERLESNETPVAMARLMHELNVAMPA
                     DGILVADGGFAAHWGGLLYDTKQAGRGFVPDRGFASIGYGLPGAMGAAMAANGRAVVS
                     ITGDGGFNMMIGELETARRTGLDLTIIVVNNAASGYVKALQHLLYGEGAYHASDLAET
                     NYAEVAKAFGCHGVRVEQPGDLAGALKAAMATPGPSVVDVVVTRDPAKMLPGVDNRAV
                     TVRKGDRVA"
     gene            complement(11402..>12728)
                     /locus_tag="VF226_08005"
     CDS             complement(11402..>12728)
                     /locus_tag="VF226_08005"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7073968.1"
                     /translation="CLVAGGKGRAMITANIVLVSVFLALLLVGSPIAVSLGLAGFAAI
                     AAGLDVNALQSVAQITYNSINKYPLIAIPLFVLTGVVFERAGVAARLVTFMQAIVGPR
                     RGGLAIVAILVCMILGGMSGSGPADAAAVATIMIPSMRRAGYPDAFSASVIAAGASTA
                     ILIPPSLALIIYGVLVPGTDLRSLFAAGLVPGLLVGLALIIPTLWISRKNGFDACEGE
                     KRPPFWRSFWQALPGLFAPVIILGGMRSGLFTPTEAAAVAAVYGLVVGFVARAFSLKG
                     IFEVLAESAETTAVIMIIIALAGLFAFAGSTLGAFDQAANAILAFTGDPLIVLGLVMV
                     LILFLGMLLDGPSIYLISLPLLYPVALKLGWNLTWFGVLIAINIAIGQVTPPVAVNLF
                     VTCKIAGVPIEATVHWVLWFIFSMAVVLILVIAFPELALWLPRYLGYSV"
BASE COUNT         2245 a         4122 c         3929 g         2432 t
ORIGIN      
        1 acggccaaag gctcatccag cgcagcccac agccgtcgca gctcatcatc gcttagtacg
       61 cgttcgcgcc gccgctcttg gccgatcttg cgcagccggt tggcaggatt gaagctggca
      121 tggtcttcgt cgactgccca gttcgcaacg cgattaaaga gtgcgacaac ccggttagat
      181 tggattggtc cggccttcgc ggcaacgttg tcgcggatct cgcgcacgtc cttgcgttgg
      241 atctcggcga acaggcaggc ctccagcggt tttgctaggt gcatccggta ccgcttctct
      301 tcatccgacc acgtcgccaa ctgcttcttg gcatgtccct cgagccaagc gcggaatagt
      361 tcgccaaacg tcttcggact ccgcgggtcc cccccgcgac ccgcctcagc cattatctct
      421 gcgcgccgct tgcaagcttc agcaagtccc acgtgctcag catcgccgat gacttgcgtg
      481 cgctctttgc gccgctcgcc ttttcccgtc tggtagcgca cgagccaggt cctacgtccg
      541 gtcgcgtaga cagcaagtag caggcccttt tctccctgga ttttgtagta cgtgcgcttg
      601 ccgacggcgt aggcggctgc gaccgacttg acgttccgtt tgagctcttg tgccatacgc
      661 cctctcaaaa tcctgcaccg cagctgcacc gcagaaaact gaagtcgcag tgagctcccg
      721 tgaatgtgct tgatagtaga ctataggtaa gcatatggaa caactatgat tcatgataca
      781 gcgtgagagt ggcagagaac gggtgagatg ctcgggctgt caatggcatt cacgaggtca
      841 ggggttcgat tcccctcggc tccaccaact tccaccgttg acgcgaaaag ttccaaggac
      901 gagccgccct cgcgctgcgc ggagcccgac tgcaaagcaa tcttgattga gccccgcagc
      961 aggcgggtgt agcagtctcc ggctggccgc tgtgaaccgg cggccagctg acaagaacgg
     1021 cctttctcca ggaccgcctc tggataaccc gatgagacga cttcccaggc actacgcacc
     1081 attcgtttat ggcgtgatcc aggctgcgat cacatcggcg gtggcaacca ttatcgcgac
     1141 atatcagatg gccagcctcg atctgcactt catcatctac tggctccagt gctggacgct
     1201 ctcggtgctg accatgctgc ctgtcgtgat cctcgtctcg ccgctcatcc agcgcgccgt
     1261 attctcgctg accgaggcga agacaccggg cgaaagcgtc taatacgggg agccgccccg
     1321 cctcaccgcg cccttgcgga aaatctgagc ctggccgggc aggaagcccg gccagcgctc
     1381 catcgccata caggggcttt cttaactcag agtgtctgcc cgccatcggc gatcagagtc
     1441 gttccggtcg tgaatgccga ctcgtcgctc gccagataaa cggcggccgc cgccatctcc
     1501 tcgactgtgc cgagccttcc catcggttgg cgacccagga agacacggcg catctcctcg
     1561 ggatcatcgg cagcctggat ccgggcttcc agtgacggcg aatgggtcgt gccaggacag
     1621 agcgcattgc agcgcacgcc cgtcttgatc acgtcccggg cgacggcctt ggtgagcccg
     1681 ataacggcgg ccttgctggc gccataggct gcgcggttcg ccacccccgt gatgctggat
     1741 gcgaccgacg cgacattgat gatcgaaccg cgaccgcgcg caatcatgcc gggcaacacc
     1801 gcgcggatcg tgtggaacat cgaggtgacg ttctggtcga agcttctggc ccaatcctcc
     1861 tccgtgcaat cgagaattgt tccattgtgc acccagccgg cgcagttgag cagcacgtcg
     1921 acctcgccgg cgtcggcgat cgcgcgctga acagcagcgc cgttcgtcac atcgagctca
     1981 acgggcctga tgcgagcatc gagccctggc aggtcctcca ttttcgaaag cgtacggctc
     2041 gcggccacca cgctcgcgcc ttcacgcgca aaggcaagtg ctgcggcccg gccaatcccc
     2101 tgcccggcac ccgtaatgaa cgcccgcttg cctacgagac gacccgtcat cgctgaacaa
     2161 cctttcttac cgattgcctt ctcgcatgga cgtacaaggc caatgcagga aagtcgaccg
     2221 ttccaagccc gaaccttgtg gccggaggcg tcgttgctgc aaggtaaagg acagcccgaa
     2281 acactgatcc gagttcaccg accgcctcgc ggagttaacc ctggatgcag aggatcgtcg
     2341 ccagcctgtt gctcgccttg gcgctttcgg tgccagtctc cgcgccgggt tggcgggctg
     2401 cggctcagcc ggttcacatc tcgatttcct gcggcgcgct cggcgtcgag ctgaagcttt
     2461 gcacggaggc gatcgaggaa tgggagcgcc gcacaggtca caaggcaacg atcgttacta
     2521 cgcccaactc gtcgaccgac cggctggcgc tctatctgca gatgctcgcc tcaggcgcgg
     2581 acgacatcga tgtgttgcag atcgatgtcg tctgggccgg catactcgcc agccacctca
     2641 tcgatcttgg cccgtacgtc ggaaatgcac cgaagcagca cttccctgtc ctcatcgaga
     2701 acgacaccgt cgacgggcgg ctggtcgcga tgccgtggtg ggcggatgtc ggggtgctct
     2761 actatcgcaa ggacctgctc gcgaaacatg gtttcacgcc accccgcacc tggcaggaga
     2821 tgactgagac cgctcgagcc attctgcagg ctgagcgcgc ggcaggcaat cgcggcgtat
     2881 ggggcttcgt tttccagggc aaggcctacg agggcctcac ttgcaatgcg ctggaatgga
     2941 tcgactcctt cggcggcggc accttcgtca acgacaaagg caagatcact gtcgataacc
     3001 cgcgcgccat cgctgccttg gcgctcgccg ccagctgggt tggcgatatc gcaccgcccg
     3061 gcgtgctgaa ctacgacgag gaggccgcgc gcggcgtttt ccagaccggc aatgccgtct
     3121 tcatgcgcaa ctggccttac gcctggacgc tggcgcaggg aaaggacagt cccgtgcgcg
     3181 gcaaggtcgg cgtcgtgccg ctgccgaagg gcggccctga cggcaagccg tccggggcat
     3241 tgggcggatg gcacctggcc gtctcccgct actccaagca cccgaaggag gctgccgatc
     3301 tcatcacgtt cctgaccagt gcggagcaac agaagaagcg cgccatcgaa gcttcctaca
     3361 atccgaccat tccggcgctt tacgaggatc ctgaaatcct cagagccaat ccgttcttgg
     3421 gtgagttgct cgagcctctg cgcaatgcgg ctgcacgacc gtcgcgtatt gccggcccgc
     3481 attacaatcg cctgtcgagc gatgtctgga gggcggccca cgacattctg gccggagcgc
     3541 cggccgagat gcgcgtggcg cagcttgcag ccgagctcga tcggctcaga tacagggcag
     3601 gctggtgata cggaaggagc tctccatttc cgcggaacgg aagcgggctg cctggctttt
     3661 cctggccccg atgctggcgg tgctggtcgt cgtcgccgcc tggccgctgc tgcggacgat
     3721 ctatttcagc ctgaccaacg caacgctggt ggacatgggc cgctacgatt tcgtcggcct
     3781 gtccaactat gtcgcttacg acaacggccg ctggtacggc gtgctgaccg atccgcaatg
     3841 gctgatatcg ctgcgcaata cgctcgtttt cgcgatcgtt tccgtttctc tcgaaatcgt
     3901 cctcggcgtc tcgatcgcga tgctgctgaa cgcgaaattt cccggccgcg ggatgctgcg
     3961 agcagccatg ctggttccct gggcgatacc aaccgtggtt tcggcgcgga tgtggggctg
     4021 gatgctcaat gaccagttcg gcgtcgtaaa cgatgcattg atgaggctcg ggctcatagc
     4081 cgagccggtc gcctggctgg cagatcctac tctttcgatg atttcggtca tcctagttga
     4141 cgtgtggaag acgacgccgt tcgtggccct gctggcgctt gccgccctgc agatggtgcc
     4201 gaaggacgtc tacgaggccg cccgggtcga cggcatccac ccggtcaagg tcttcttcca
     4261 ggtgacgctg ccattgatcg gcccggcgct ggccgtggcc gtcatcttcc gcctgctcga
     4321 tgccatgcgg atgttcgacc tcgtgtacgt catgacgggc acgaccgacg ccacgatgac
     4381 tctctccgtc tttgcgcgcc gcgagctcgt ggatttccag gacgtgggct acggatccgc
     4441 cgcctcgacg ttggtgttct tcatcatcgc cctgctgacc gccctctacg tcatggccgt
     4501 ccgactcgat cttaatggga ggcgccgatg aatcgggtgc ttaccgggac agggttggcg
     4561 atcgcctgca tcgtgctggc ggtcttcctg ctgttcccgc tctattacgc ggtgctgacc
     4621 tccttcaagg cgggctcaga gctgttccgc gtcgactact ggccgacgga catcgacttc
     4681 tcgagctacg tccgcatctg gcaggagcag ccattcgcgc ggaacatctt caactcggtg
     4741 ctcgtcgcga ccttggtcgt gctgctgtcg ctgctgctgt cgctgactgc tgcctatgcc
     4801 ctcggccgcg tccagtttcg tggccgcaag ttcctgcttt atacgatcct cggcgtctcg
     4861 atgtttccgc aggtcgccgt cctttccggc ctcttcgagg tgatccgcgc gctcgggctc
     4921 tacaataacc tcggcagcct tgtcctctct tatctgattt tcacccttcc gttcacggtc
     4981 tgggtgctga ccacattcat gcgcgacctg ccgttggaga tcgaggaagc ggccatcatg
     5041 gacggcctca gcccgttcgt catcgtcacg cgcatcttca tgccgctgat ggggccggcg
     5101 atggtggcga ccgggttgct cgcattcatc gccgcgtgga acgagttcct gttcgccctg
     5161 accttcacgc tgtccaacga acagcggacc gtgccggttg cgatcgcgct catcaccggg
     5221 gcgagcgagt atgagctgcc atgggggaat atcatggcgg cctcggtcat cgtcacgctg
     5281 ccgctgatcg cactcgtcct gatctttcag cggcggctgg ttgccggtct gacggccggc
     5341 gcggtgaaag gatgagcgat ggcgggggta cgcctggaaa aagtcaccaa gcgctttgga
     5401 cgtacggagg tcatccgcga cgtcgacctc gaagttgcgg acggagaatt tctggtcgtc
     5461 gtcggaccgt cgggctgcgg aaagtcgacc gttctgcggc tcatcgccgg cctggaggaa
     5521 gttacctccg gcaggatctg gatcggcggc aaggacgtca cgaacctgcc gcccgcgaag
     5581 cgcggcgtgt cgatggtctt ccaatcctac gcgctttatc cgcacatgga cgcctacaag
     5641 aacatcgcct tcggcctaca gatgtccggg acgagcggcg aaacggtcga ccagcgcgtc
     5701 cggcgggtcg cccggatgct gcagatcgac gacctcttgc accgcaagcc tcgcgagctt
     5761 tccggtggcc agcgccagcg cgtcgccatc gcgcgcgcta tcgtgcgcga gccgcaggta
     5821 tttctgttcg acgagcccct gtcgaacctc gacgccgctc tgcgggcaca aacgcgcctg
     5881 gagctcacgc gcctgcacga cgagctgaag gcgacgatga tctacgtcac gcacgatcag
     5941 ctcgaggcga tgacgctcgc cgaccgtatc gtgctgctca acgccggccg tatcgagcag
     6001 gccggaaagc cgacagagct ttaccagcag cccgccaccc gcttcgttgc ggagttcatc
     6061 ggctcgccga agatgaacat tctgcccgtc accgtggagg ccggcgtggt gatcctgccc
     6121 tctggcgagc ggctgcaggc acctgtagcg catgacggtc ctgccgacct cggtattcgc
     6181 ccggaaaaca ttgcgcttgt cacgcgcgag gaggcgaatg ccatccgcgc ccgggtgctg
     6241 cacgtcgacg agctgggaga gtcccggatc attcacgctg cgctagacgg ccgaacgacg
     6301 ctcgccattc gtcatccggc cgataggccc gcaccgagca aaggcgacga gatcgccgtg
     6361 cggctcgacc ccaggcatct gcaccttttc gcgccggacg gttcgcgggt gcagtagcgc
     6421 accagtcgca gaatcgatcg cacagtcgta ttgcggcctc ccgctctccg tgttctgatc
     6481 gcaagaacaa tgatcaaaaa accacggagg aaatgatggc caaatctctc ttggcaatgg
     6541 tggccggagc gtcggtggtg gcgctcggct tgtcgctgcc gattggcgat gcgaacgcgc
     6601 agaccacctt cgccggcaaa cgcatccagg tgatcgtgcc attcgcacca ggcggtgcga
     6661 ccgacgtagc cgcgcgcttc ctcgagaagt accttgagcg gcacctcgac gggaatccgg
     6721 acgtcgaagt cgtgaaccgt gcgggtggcg gctcgattct cggtgcgaac tggttccagg
     6781 agaatgccaa gcccgacggt gagactgtcc ttttcaccac gtcctcgacg gccaacccct
     6841 atgtgctcgg ccagaaagag gtgaaatacg acctcgcctc gatgcgcgtc ggctattcgc
     6901 tgcccttcgg gagcgtgact tacgtttccg cgaaaaccgg catcaagtcg gttaaggacc
     6961 tcaagaatac gaagacggag ctcatctacg gcggcatcgc ggcagcggcg agcgatctac
     7021 cgacactgct ctccttcgag gtgctcgatc tcgatgtgcg cgcggtgctc ggcttcacgg
     7081 gtcgcggacc catccgcctc gccttcgatc ggggcgagac caatctcgac ttccagttca
     7141 ctcccgtcta cgagactcag gtggccgacc aggtcgcctc cggaaaggtg atcgcgctga
     7201 tgaccggcgg ctccgtcggg ccggatggca agctgactgc tcgcgacccg gcggtacctg
     7261 atatcccgac ggtctatgag gtctacaagg agctgaacga cggcaaggag ccgagcggcg
     7321 tcgagtggga tgcctatcaa tcgatcgggg cactgacgct cggctacggc ctcaccgcct
     7381 atctccacaa ggatacgccg gacgaggtca tcgagctctt cgccaaggcc gtcgagcgga
     7441 tcaatgccga ccccgagttc ctgaaggaag gcaaggaagt catcggcggc tacaagctca
     7501 tatccccgaa ggatgccgaa aagccgctga aagctgcgct ccagccttct gaagaggtga
     7561 ggagctacct tcgcaaattg ctgtctgaaa agtacggcgt caccttctga ggcctctatt
     7621 cagtgcggcc gggcttccgg ccgcaattcc atacgagctc gcccatggaa catgcgatcg
     7681 aggcactggc cgtgctgttc gacccgctcc ggcttgtggc gctgttcgcc ggcatgctgg
     7741 cagggatgat ctgcggaatg ctgcccggcc ttggcggcgt cgcgacggcc tcgatcctgc
     7801 tgccctttgt ttttctcctc gatgactatt cggggctggc gctgctgctc ggcgcccttt
     7861 ccgtcgtcta cacgtccgac acgatcacct cggtgctcgt cggcgcaccc ggctcaccgg
     7921 cttcggcccc cactgccatc gaggggcacg cgctcgcaag acaaggcaag gccgcaatgg
     7981 cgctgggcgt gggctttctc gcctccatgg cgggcggcct gatcggagcc gtgacgctcg
     8041 cctttgcgat cccgatcgca gggccgatcg tgctggcact cggcaccccg gagctgttca
     8101 tgttcgcggt tgtcgggttg tatttcgcct cgagcatgat cgggcgcgat ctggcgaccg
     8161 gcgttctcgc cgggtgcatc ggcattctgc tcggcgtggt cggggccgcg ccggcagcag
     8221 cgaactaccg gttcacgttc gggcaggcct atctgctcga cgggctctcc ctgacgatcg
     8281 tcgcgctcgg gctgtttgcc gtggcggagg tgatcggcat gctggcctcg ggcggaggga
     8341 ttgcaagtgg tcgccagctc ctgacagatt ggggtgccgg cttcaccgag ttctggcgca
     8401 accgatggct cattctgcgc tcatcgatca tcggggtatt cggcgggttc gttccggcgg
     8461 tcggcgcgag tgcatcgacc tgggtcgcct atgggcacgc ggtgcgctcg acgccggaca
     8521 aatcgcgctt cggccacggc gaaatccgcg gcatcgccgc ggcggaaggc gccaacaact
     8581 cgaccgtcat ttcggacctt gtgccgacgc tcctgttcag cgttcccggc ggtcctgcgg
     8641 ccgcaatatt tctcggcgcc cttttttcat tcggtttcta tcccggcccg cgcatggtca
     8701 gcgagcaccc gacgctgatg tacgtcatcg tctggagcgt cgccctggcc tctatcatcg
     8761 gtgccgcgat ctgttttgcg gtcacaccat tcattgcgcg aatgacgcag atccgtttcg
     8821 cgctgatcgc cgcgccgctc gtgctcatca tggtggcggg cgcctatcag gcgacgcagt
     8881 cgctgggtga cattgccgtt cttcttattc tcgggctcgt cggctggctg atgaagcagg
     8941 cggactggcc gcgcgcgccg gtgctcgtcg gtttcgtgct tgcggcaccg atggagcagt
     9001 acttctggtt gacgacccag attcacgggt ggagctggct cttgcgcccc ggcgtgctca
     9061 tcatcgcatc gttcattgtc gtgccgctgt tcattcagct cacgcagcat tggcgcggtg
     9121 gacgtgccga gcgcatcgag gagagtgcaa atccccgaag cacgacctca gcccagcacg
     9181 gcaccacgtt tacgcgctgt gtcctggcgt cattggccgt acccgtattt gcttacgccc
     9241 tgtgggagac gatgcagttc tctccgagct cgcggttgct gccgctgctg gccatcgttc
     9301 ccggtctggc actcgcaata tttatccttg gccgcgagct gtggcggctc aagacgcacg
     9361 tcgaagaatt tcggttcgac gcaacggacg agtacggcgt tattctaggc ttcgtcgtct
     9421 atggaatcgg gatcgcgctt ctcggcttca acatcgcaac gatcggattc ctcgggtggg
     9481 tgcttttcac ctgtgcgaaa atgcggccca taacgggttt catttacggg gtagttctta
     9541 tcatcgcgat taatggcata tttcacctga tgcgcgtcgc accgcccgat ggcctgctga
     9601 tgagctcgag cctgttctag cccaagccgc ccaacatagg ccgatgtcgt cgaaggtcac
     9661 gccacgcgat cgcctttcct gaccgtcacg gcccggttat cgacgcccgg aagcatcttc
     9721 gccggatcgc gggtgacgac gacatcgacg acgctcgggc ccggcgtcgc cattgccgct
     9781 ttgagcgcgc cggcaagatc gccgggctgc tcgacgcgaa cgccgtggca gccgaacgct
     9841 ttcgcaacct cggcatagtt tgtctccgca agatcggagg cgtgataagc accttcgccg
     9901 tagagcagat gctgcagcgc cttcacgtaa ccggacgccg cgttgttgac gacgatgatg
     9961 gtgaggtcga gcccggtccg gcgcgctgtt tccagctcgc cgatcatcat gttgaaaccg
    10021 ccatcgccgg tgatgctgac cacggcacgg ccattcgcag ccatggccgc acccattgcc
    10081 cccggcaggc cgtagccgat ggaggcgaag ccacgatccg gcacgaagcc gcggccagcc
    10141 tgcttcgtat cgtaaagcaa gccgccccag tgcgcagcga acccgccatc ggcgacgaga
    10201 atgccgtcgg cgggcatggc gacgttcagc tcgtgcatca ggcgcgccat ggcgacggga
    10261 gtctcgttgg actccaggcg ctcccgaacc ccttcgcgcc aagcctgcat gcgcttggcg
    10321 acatctgccg catagtccgc ctgccgctcg cggatggcgg ccgcgtcgct tgaaagtgcg
    10381 gtcagcagat cgcggatgcc ctcgcgcgcg tcgccccaca gcgccacttc cggttcgcgc
    10441 gtgcggccaa actcctcggc caggatatcg agatgaatga tccgcttgcc cgcgggcgga
    10501 accgtgtaac gcttggtcgc gatctcgccg agcttgcagc ccacgacgat gatgagatcg
    10561 gactcgtcga tcagctggtt cgcgatgcga tcgtatcgcc cgaacaggcc ggcgttgagc
    10621 cggtcggtgc atggaacagc gcctttgccg gtcatggtgt gggcgacggg cagattgata
    10681 gcgcgagcca gctcggtgag cgcttccgcg gcgcctgaaa tatggatacc ccctcccgcc
    10741 agcaggatcg gccgcttcgc ctcagcgatc atccgtgcag cgcgttccag cgaacgggca
    10801 tcaggccggc aacgcagagc cggcgcggcc tgataagcca gatcggcgtg gaagtcggac
    10861 tcctcgtagc catgcgtgcc gtggcaaacg tcttccggaa tgtccaccac caccgggccc
    10921 ggccgtccgg tcgtcgcgac ggcaaacgca cggcgcatca gctcagggat gcgctgtacg
    10981 ctctcgacgc ggatcacttc tttcaccgcg ggccgcagga tttcgagctg acgcgattcc
    11041 tgggtcatgt tcttccagga atggtcgcgg tgcgtatcgc ccgtcagcac gaccagcggc
    11101 gagcccgcat tcagcgcctc gacaaggccg gtgacgaggt tcgtcgctcc agggcccagg
    11161 gtggcgtcgg cgagaccgac tcgtccggac accttggcat aggcatcggc cgcgaacacg
    11221 gcggcgcgtt catcgttgat cagatgatgc gcaaggccca gccggcgtgc cgcgtcgtag
    11281 aagggcagca gctggaagcc gcccatgccg aacatcggcc cgccctcgtg cgcctggatc
    11341 atgcgcgcca aggcctcgcc gccggtcatc tcattcgcgg acattcagga ctccttggtt
    11401 cttagaccga atagccgagg taccggggta gccagagcgc gagctccggg aaggcgatca
    11461 caagtatgag cacaacggcc atggagaaga tgaaccacaa tacccagtgc acagtggcct
    11521 cgatcggcac gccggcaatt ttgcacgtga cgaacaggtt cacagcgacg ggcggcgtga
    11581 cctggccgat ggcaatgttg atcgcaatga gcacgccgaa ccaggtgaga ttccagccga
    11641 gcttcaacgc caccggatag agcagcggca gagaaatcag ataaatcgaa ggcccgtcga
    11701 gcagcatgcc gagaaacagg atcagcacca tcaccaggcc aaggacgatc agcggatcgc
    11761 cggtgaatgc cagaatggca ttggccgcct gatcgaaggc gccaagggtg gagcctgcga
    11821 acgcgaacag gcccgccagt gcgatgatga tcatgatgac ggccgtggtc tcggcggact
    11881 cggcaagtac ctcgaagata cctttcaggc tgaaggcacg tgccacgaag ccaacaacaa
    11941 gaccatagac ggcagcgaca gccgccgctt ccgtaggcgt gaaaagtccg ctcctcattc
    12001 cccccaatat gatcacgggc gcgaacaggc caggtaacgc ctgccagaac gagcgccaga
    12061 acgggggacg cttctcgccc tcgcacgcat cgaagccatt tttgcgactg atccacaaag
    12121 tcgggattat cagcgcgagg ccgacgagaa ggccgggcac cagaccggcg gcaaacagcg
    12181 acctcagatc ggtgcccggc acgaggacgc catagatgat cagcgcgagc gacggtggaa
    12241 tgagaatagc cgtcgacgca cctgcggcga tcacgctcgc ggagaaagca tcgggatatc
    12301 cggcccgccg catgctcggg atcatgatgg ttgcgaccgc agccgcatca gcggggccgg
    12361 agcccgacat gccgcccagg atcatgcaga cgaggatcgc aacgatcgcg aggccgcccc
    12421 tgcgcggccc gacaatggcc tgcatgaagg tgaccagtcg cgccgcgacg ccggcccgct
    12481 cgaaaacgac acccgtcaga acgaatagcg ggatggcgat cagcggatac ttgttgatcg
    12541 agttgtacgt gatctgcgca acgctctgca gtgcgttgac atcgaggccg gccgctatcg
    12601 ctgcaaagcc cgccaagccc agcgacactg caatcggcga tcccacgagc agcagggcca
    12661 aaaagacgga aacgagcacg atgttcgcgg tgatcatcgc gcgccccttt cctcctgcca
    12721 ccaggcaa
//
LOCUS       DASVDU010000126         9518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1439617, whole genome shotgun sequence.
ACCESSION   DASVDU010000126 DASVDU010000000
VERSION     DASVDU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439617"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF226_08010"
     CDS             <1..338
                     /locus_tag="VF226_08010"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7073969.1"
                     /translation="LMMMLIILVLGMFMDDWAIIMLCTPLFIPIIDALGVDKLWFGAL
                     FIVNIQIAYLSPPFGFVLFWIKSVLPPDVTMGDVYRSIGPFVLLQLLGLTLIFIFPEI
                     AVWLPNALK"
     gene            complement(488..1552)
                     /locus_tag="VF226_08015"
     CDS             complement(488..1552)
                     /locus_tag="VF226_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7073970.1"
                     /translation="MKNSKISKRDFLRKSILTGAGTVGATTLAAPYVKSQTPIKWRLQ
                     TYAGPALAEHVCKPGIDAFNKAANGEMVIEMYTADQLVPQGELFRAVQAGTIDAAQSD
                     DDSAAAPVDVAVFGAYFPFASRYSLDVPVLWHWYGLNKIWEEAYGEVPGVTWLSAGAW
                     DPCNFATTKPIRSIKDLEGLRIYMFPTGGRFMQRFGVVPVSLPYEDVQVAIQTGELDG
                     VCWCGATETYTVGWADVTKYYLTNNISGAWSGSYFANTDKWNALPEHLKTLFRMSMDT
                     SHYYRQHWYWWGEAHYRTTGGKLELTTIPEDEWKIIEDEAMKFWDEIAAQSPRNAKVV
                     EILKKYQETMRKAGAPYRYG"
     gene            complement(1690..3015)
                     /locus_tag="VF226_08020"
     CDS             complement(1690..3015)
                     /locus_tag="VF226_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7073971.1"
                     /translation="MTSEFITLFMFGTMMVLLFTGQRVFGVIGFVGAAAALWLWGKGS
                     FEMPFNASFQVLNWYPLITVPFFIFMGYMLSESGVANDLYRLFHVWFGPVRGGLAMGT
                     IGLMVMIAALNGLSVAGMAIGVTIALPQLLKRGYDKVMVTGVIQAGSSLGILFPPSVV
                     LVLYAMIARQPVLSLWLAGVLPGLIMAFIFGLYIYTRSLIQPHIAPAMPAEERAQITW
                     AERFSLLRKGVLPLAVFLIMMGLFMTGTTSLVESSAVGAALSILVAWYAGNLNRKVLE
                     DSTRNTLAISCMFLWIILAALCFSSVYDGLGAVRALKTLLVDTWAFDPWTILILMLCS
                     FIVMGMFLDDTAMLVIVAPLYIPLVKSLGFNTIWFGVLYTITCQIAYITPPFGYNLFL
                     MRAMAPPDIKLIDIYRSIIPFFFLMILTMIIVMMFPQIALWLPETYRRW"
     gene            complement(3012..3455)
                     /locus_tag="VF226_08025"
     CDS             complement(3012..3455)
                     /locus_tag="VF226_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7073972.1"
                     /translation="MYLLFVMGAMLLYSTLSRLFFGIPINWVLEMTQFMLSAYYLLGG
                     AYTLQLNQHVRMDLFYGRLTPRGKAITDAITILFVLFYLAMLFGGGISSTHYAIAYGQ
                     KNYSAWAPLLWPIKVVMTTAIFLMLLQCISTLIKDVAAARGKPIA"
     gene            complement(3537..4298)
                     /locus_tag="VF226_08030"
     CDS             complement(3537..4298)
                     /locus_tag="VF226_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX7073973.1"
                     /translation="MDSVRVTNPDGRGAYVLTCDHASNYLPPEFGSLGLPDSELSRHI
                     AWDIGALPVARRMAEMLDAVLVETCVSRLVLDCNRALDSPGLIPTVSETTKIPGNTNL
                     SDAERKGRIERFWRPFHDVIETVIQKKLARGQESRLVSVHSFTPVYKGAARPWQIGII
                     HDEDARLALPLIRALQRSGEFAVAANKPYSPADGVYFTLEEHARSRGLPCAMIEIRND
                     EIGDAAGQAAWAARLASALAGIGGLAAPSAAHQLP"
     gene            4463..5293
                     /locus_tag="VF226_08035"
     CDS             4463..5293
                     /locus_tag="VF226_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7073974.1"
                     /translation="MGASVAELITQRMETMPAGERRAAQALVASYPLIGLKTVAEFSQ
                     AAGVSPPTVLRFVARLGFQNYSEFQSALQDELAAQLQSPASRTHGPTTSSLEGTLPPL
                     LEATLENIRVTFRHVSERQFAEIVGKLADRRGHMFLLGGRFTDPLARYMAAHLTIVRP
                     RVFHLAGQESMWRDRLIDMGKRDVLLIFDIRRYQENLLRFAETAHRRGVQIILFTDQW
                     LSPIARFARHVIAGRTAVPSPWDSSAALFVMAEALISALTLRLENESAARIQEMENLR
                     "
     gene            complement(5404..5808)
                     /locus_tag="VF226_08040"
     CDS             complement(5404..5808)
                     /locus_tag="VF226_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073975.1"
                     /translation="MAAIVEALRSIRAGVLPQRTSLHTASKTGLKVRAPAPKLWGTLI
                     LVGFATLSLGAWWVSTSDSSDAELRAELAATREELRTAREVLRLRARAIEMAENEVKV
                     LRKELANVRAAANAPGVPVFAADGAWPEKRTR"
     gene            complement(6190..7980)
                     /locus_tag="VF226_08045"
     CDS             complement(6190..7980)
                     /locus_tag="VF226_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX7073976.1"
                     /translation="MTFTTRPEITGTFGVATSTHWLASAVGMSVLEKGGRAADAAVAM
                     GFVLNVVEPHLNGPLGEVPLLVRPADAEAPTVICGQGVAPAKATIAHYRAQGLSEVPG
                     SGMLATVVPGAFDAWMLLLRDYGTMRLRDVLEPAIYYAEAGHPILARAADAIAGLADF
                     FKAEWPTSAEVWLPGGAPPAPGQLFRNPALAATWRRLLAEAETRSGREEQIEAARAAF
                     YRGFLAEAIGRWVETAEVMDAAGGRRKGVLTADDMAAWQATTEAPLAVDYNGWTVWKA
                     GVWSQAPLLLQSLRILEGTNIGEVDPAGADFVHLVTETLKLAMADREAYYGDPAHTEI
                     PVETLLSRSYAEQRRALIQATASREQRPGRIPGYEAWAEASIARAARRVAAEAGAGVG
                     EPTMAHLSSRRSDTVHLDVIDRWGNMVSATPSGGWLQSSPVVPGLGMPLNSRAQMFWL
                     DEGLPTSLAPGRRPRTTLSPSMAERADGARLAFGTPGGDQQDQWQLILFLRLAHHAMN
                     LQQAIDAPLFHTGHVTGSFYPRTARPGHLMAEPAFGQGVLDALRDKGHELEVSEPWAI
                     GRLTAAQRDADGLLRAAATPRLMQAYAIGR"
     gene            complement(8004..8861)
                     /locus_tag="VF226_08050"
     CDS             complement(8004..8861)
                     /locus_tag="VF226_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073977.1"
                     /translation="MPAGEEIAPQERRALAKFLRNRTAVFGAALVAAFVLMAVLAPLI
                     APYDPTKTNFAALRKAPSALYWLGTDELGRDILSRVMYGGVASLYAGVVSVAIALAIG
                     MPIGLVAGWFGGWIDAVIGRATDALLAVPFLILAIALAAALGPSLTNAMIAIGLSQVP
                     IFVRLTRGQVLAVKAEEYVEGARAVGVPRWLILARHVAPNITAPLIVQATLTVATAII
                     AEASLSFLGLGQQPPAPSWGSMLNTAKNFLEQAPWMSLSPGIAIFLVVLGFNLLGDGL
                     RDALDPRER"
     gene            complement(8891..>9518)
                     /locus_tag="VF226_08055"
     CDS             complement(8891..>9518)
                     /locus_tag="VF226_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7073978.1"
                     /translation="IVIALAIGIPMGILSAVRKGTWIDAVTSMVSLSGLSIPNFWLGI
                     ILILVVAVNLQWLPASGFVSVFEDPARAFQTTIMPAFVLGSGLAAVLMRHTRSAMLEV
                     LSLDYIRTARAKGLLPRVVILKHAFRNALVPIVTLATLLFGQLLAGAVLTEQVFTIPG
                     FGKLIVDAVFNRDYGVVQGVVLCTAIAFILMNLVADVLYIVINPRLRT"
BASE COUNT         1790 a         3024 c         3017 g         1687 t
ORIGIN      
        1 tgctgatgat gatgctcatc atcctggtcc tcgggatgtt tatggacgac tgggcgatca
       61 tcatgctgtg cacgccgctg ttcatcccaa tcatcgacgc gctgggcgtg gataagctct
      121 ggttcggcgc gctgttcatc gtcaacatcc agatcgccta tctgtcacca cccttcggtt
      181 tcgtcctgtt ctggatcaag agcgtgctgc cgcccgatgt caccatgggt gacgtctacc
      241 gctcgatcgg accattcgtt ctgctccagt tgctcggcct cacactcatc ttcattttcc
      301 ctgagattgc cgtatggctc ccgaatgcgc tgaagtgatg ctccgtctag ctagacgaag
      361 cggtgacaga aactcggtat ttgagcagga aggtgaggac aaaattattc tgagaaacaa
      421 acggagccgc tgtcaccttg agaaatgaac aatgcctggc cgcagagcgg ccaggccgat
      481 ccaaagttca cccgtagcga tagggtgcgc cggccttgcg catcgtctcc tgatatttct
      541 tcaggatttc gacgaccttt gcgttgcgag gactctgggc ggcgatttcg tcccagaact
      601 tcatggcctc gtcctcgatg atcttccact catcttcggg aatcgtggtg agctcgagct
      661 tgccgccggt ggtgcggtag tgcgcctcgc cccaccagta ccagtgctgg cggtaataat
      721 gggaggtgtc catggacatc ctgaacagtg tcttcaggtg ctccggcaac gcattccatt
      781 tatccgtgtt ggcgaagtat gagccggacc aggcgccgga gatgttgttg gtgagatagt
      841 acttggtcac gtccgcccag ccgaccgtgt aggtctcggt ggcgccgcac caacacacac
      901 catcgagctc gccggtctgg atcgcaacct gtacgtcctc atagggaagc gaaaccggta
      961 caacgccgaa cctctgcatg aagcggccgc cggtcgggaa catgtagatg cgaaggccct
     1021 ccagatcctt gattgacctg atcggcttcg tggtggcaaa gttgcaggga tcccaggcgc
     1081 cggcgctcag ccacgtcacg ccgggaacct cgccataagc ctcttcccag atcttgttca
     1141 gtccgtacca gtgccacagg accggaacgt ccaggctgta gcgcgatgcg aaggggaagt
     1201 aggcgccgaa tacggccaca tccaccggtg cggcggcgga atcgtcgtca ctctgcgccg
     1261 catcgatggt gccggcctgc acggcgcgga atagctcgcc ctgtggaacg agctggtcgg
     1321 ccgtgtacat ttcgatcacc atctctccat tggccgcctt gttgaaggcg tcgatgcccg
     1381 gcttgcacac gtgctcggcc agcgccgggc cggcgtacgt ctgcagccgc cacttgatcg
     1441 gagtctgcga tttgacatag ggggccgcca gcgtggttgc gccgaccgtg ccggcgccgg
     1501 tgagtatgga ctttcggaga aaatctcgct tgctgatttt ggagttcttc atgtccgtcc
     1561 tccttcatcc gagtggcatt ctctcattcc ggtcagcttc cgcgcattcc gcgctctgcc
     1621 ggccgcattt catctccgtc tgctgtcctg tatccgcttt cagtggaggc cagtccgccc
     1681 accctaagat caccacctcc tgtaagtctc cggtagccac agcgcgatct gcgggaacat
     1741 catcacgatg atcatggtca ggatcatcaa gaagaagaac gggatgatcg agcggtagat
     1801 atcaatcagc ttgatgtccg gcggcgccat tgcacgcatc aggaacaaat tgtagccgaa
     1861 cggcggcgtg atgtacgcga tctggcaggt gatggtgtag agcaccccga accagattgt
     1921 gttgaagccg agacttttga ccagaggtat atagagcggc gccacgatca ccagcatggc
     1981 ggtgtcatca aggaacatcc ccatgacgat gaaggagcac agcatcagga tcaggatcgt
     2041 ccagggatcg aatgcccacg tgtcgaccag gagcgttttc agcgccctca ccgcgcccag
     2101 cccatcatag accgagctga agcacaaagc ggccaggatg atccacaaga acatgcatga
     2161 gatcgcgagc gtgttgcgag tggaatcctc caacaccttg cgattgaggt tgccggcgta
     2221 ccacgccacc aatattgaga gcgctgctcc gacggccgag ctttccacga ggctcgtcgt
     2281 gccggtcatg aacagaccca tcatgatcag gaagacggcc agcggcagga cgcctttcct
     2341 gagcagcgag aagcgctcag cccaagtgat ttgtgccctc tcttcggccg gcatggccgg
     2401 ggcgatatgc ggttgaatga ggctgcgcgt gtaaatgtaa aggccgaaga taaatgccat
     2461 gatcaggccg ggaagcacgc cggcgagcca caagctgagc accggctggc gcgcgatcat
     2521 ggcgtagagc accagcacga cgctcggtgg aaagaggatg ccgagcgacg atccggcctg
     2581 gatcacgccc gtcaccatca ctttgtcgta accgcgtttg agcagttgcg gcagcgcgat
     2641 tgtgacgccg atcgccattc cggccaccga caggccattg agcgcggcga tcatcaccat
     2701 cagtccgatg gtgcccatcg ccaaaccgcc ccgcaccggt ccgaaccaga cgtggaacag
     2761 tcggtagagg tcattggcga cccccgactc cgacagcatg taccccatga agatgaaaaa
     2821 gggcacggtg atcagcggat accagttcag cacttgaaag ctggcattga agggcatttc
     2881 gaacgagcct ttgccccaca gccacagcgc tgctgccgca ccgacgaagc cgatgacgcc
     2941 gaagacgcgc tgaccggtga acagcagcac catcatggtg ccgaacatga aaagggtgat
     3001 gaattcggag gtcatgcgat cggcttcccg cgggcggctg caacatcttt gatcagggtg
     3061 gagatgcact gaagcagcat caggaagatg gccgtcgtca tgacaacctt gatcggccag
     3121 agcagaggcg cccaggccga atagtttttc tgcccgtaag cgatcgcgta atgcgtgctg
     3181 gaaatgccgc cgccgaacag catcgccaag taaaacagca cgaacaggat ggtgattgcg
     3241 tccgtgatcg ctttgccgcg cggggtcagc cgcccataga acaggtccat gcgcacgtgc
     3301 tgattgagtt gcaacgtata agcgccgcca agcagataat aggccgagag catgaattgc
     3361 gtcatttcca gcacccagtt tatgggtatg ccgaaaaaga gccgcgacag cgttgaatag
     3421 agcagcatcg cgcccattac gaacagcaga tacatcgcgc agcggccgat ccggtaattc
     3481 accgcatcga cgtatcggac gaaagcgagt gcggctttcg gcaagctctt gacctcctat
     3541 ggaagctgat gcgcggcaga cggtgccgcg agtcctccga tgccagccag tgccgacgcc
     3601 aaccgcgccg cccaggcggc ctgccccgca gcatcgccga tctcgtcgtt gcggatctcg
     3661 atcatcgcgc acggcaagcc gcgggagcgg gcgtgttctt cgagcgtgaa atagaccccg
     3721 tcagccggcg aatagggctt gttggccgcc accgcgaact cgcccgaccg ctggagcgcc
     3781 ctgatgagag gcagggccag ccgcgcatcc tcgtcatgaa tgatgccgat ctgccagggc
     3841 cgcgccgcac ccttgtagac gggtgtgaac gaatgcaccg agaccagtcg agactcctgt
     3901 ccgcgcgcca gcttcttttg gatgacggtc tcgatcacgt catggaaggg ccgccagaag
     3961 cgttcaattc gtcccttacg ctcagcgtct gaaagattgg tgttgcccgg gattttggtg
     4021 gtctcactga ccgtcggaat cagacccggc gaatcgagtg cgcggttgca gtcgagcacg
     4081 aggcgcgaca cgcaggtttc caccagcacc gcatcgagca tctcggccat gcgccgcgct
     4141 accgggagcg cgccgatatc ccaggcgatg tggcgggaaa gctcgctgtc cggcaggccg
     4201 agggagccaa actcgggcgg caggtagttg gaggcgtgat cacacgtcag aacataggcg
     4261 ccccttccgt cagggttcgt tacccttacg gaatccatgg gtgaatagcc cgctggatgt
     4321 gactccccca ttggccgcct gcccttgagg cgcgtaccat atgttacgga ttttgttcgt
     4381 ttgcgcctct acggatgatt tgatccagtt ttagatgctt tgtcaaacgt cgattgggca
     4441 ggtcacgaat gcggaggggg agatgggcgc gagtgttgcc gagctgatca cgcagcgtat
     4501 ggagaccatg ccggccgggg agcgccgcgc cgctcaggca ctggtcgcca gctatccgct
     4561 gatcggcttg aagacggtcg cggagttctc gcaggctgcg ggcgtctctc cgcccaccgt
     4621 gctgcgcttt gtggcccgcc tcggtttcca gaactactcc gagttccagt cggccttgca
     4681 ggacgagctt gccgcgcaat tgcagtcgcc ggcttcccgc acccatggcc caacgacatc
     4741 aagcctcgag ggtacgctgc ctcccttgct ggaagcgacg ctggagaaca ttcgcgtgac
     4801 cttccgccat gtttccgagc gccagttcgc cgaaatcgtc ggcaagctcg ccgaccggcg
     4861 cggtcacatg tttctgctcg gcggccgctt taccgatccg ctcgcccgtt acatggctgc
     4921 gcatctgacc attgtgcgcc cccgcgtttt ccatcttgcc ggccaggaga gcatgtggcg
     4981 cgaccggctg atcgacatgg gaaagcgcga cgtcctgttg atcttcgata ttcgccgcta
     5041 ccaggagaat ctcctccgct tcgccgagac ggcgcatcgg cgcggcgtgc agatcatact
     5101 gttcaccgac cagtggctgt cgcccattgc gcgcttcgcc cgccacgtca tcgccggccg
     5161 cacggccgtg ccctcgccct gggattcgtc ggccgcgctg ttcgtcatgg ccgaggcgct
     5221 gatcagcgcg ctgacgctgc ggctcgaaaa cgaaagcgcg gcccgcatcc aagagatgga
     5281 aaacctgcgc taagggcctc gatggccatt cggtcagctg gtccaagggc cagtggtttt
     5341 gcgcgccgag caagctcaat ccgctcggaa acgtgggctg cacatcgagc atctgccggc
     5401 aagctagcgt gtgcgctttt ccggccaagc gccgtcagca gcaaacacgg gcaccccagg
     5461 agcatttgcg gccgcgcgga cgttcgccag ttcttttctg agcaccttga cttcgttctc
     5521 agccatttcg atcgctctgg cgcggaggcg caatacctcc cgcgcggtgc gcagctcctc
     5581 gcgcgtggcg gcgagctcgg cacgcagctc ggcgtcgctg gagtcagatg ttgagaccca
     5641 ccaggcaccc agagacagcg tggcaaaacc aaccaggatc agggttcccc agagtttcgg
     5701 cgccggcgcc cgtactttga gccctgtctt cgatgcagtg tggagcgatg ttcgttgggg
     5761 cagaacgccc gcccgaatgc ttcgaagcgc ctcgacaatc gcggccatcg gcgtgtttcc
     5821 tcttaggctc gtgtgctcgg acggctgaat gcgcgtcgcc gtcgcgccgg cccaaactgc
     5881 gcaccaaacg cgagaacaca cccgcacagc ccggaatgcg caccaaaagc gtcgtcgcga
     5941 gcacgaaata ctttgcgcga agggaagcgg tccctgcgct gtggaaagtc cacgaatctc
     6001 tgtggaagaa acaatctatg gttgttgccg tgattcggct agcgcgaata gggcatccgc
     6061 cgcctgcatc agcctcagtg ctgatttcgc gctacagtcg cgcttctacg cccgcgcttt
     6121 ggtccggttt gatccggggt cgaatgggcc gacctgccgt cgcccgatta tcgtctcctg
     6181 cccactctct catcgcccga tggcgtaggc ctgcatcagc cgtggggtcg ccgccgcccg
     6241 gagcagtccg tcggcatccc gttgcgctgc ggtcagccga ccgatcgccc agggctcgga
     6301 gacctcgagc tcgtgcccct tgtcacggag cgcgtcgagc acgccttggc cgaaggccgg
     6361 ctcggccatg agatgcccag ggcgcgcggt gcgcggatag aacgatcctg tcacgtggcc
     6421 ggtgtggaag agaggcgcgt cgatggcctg ctgcaggttc atcgcgtggt gggcgagccg
     6481 caggaacaaa atgagctgcc attggtcctg ctggtcgccg ccgggtgtgc cgaacgcgag
     6541 ccgggcgccg tcggcgcgct cggccatcga aggcgacaat gtcgtgcgcg ggcgccggcc
     6601 cggcgcaagc gacgtcggca ggccctcgtc gagccagaac atttgcgccc gcgagttgag
     6661 cggcatcccg agccccggga cgacgggaga cgattgcagc cagccgccgg atggcgtcgc
     6721 cgaaaccatg tttccccagc ggtcgatcac gtcgaggtgg accgtatcgc tgcgacggga
     6781 ggagagatgc gccatcgtcg gctcaccaac gcctgcgccg gcttctgccg ccactctgcg
     6841 cgccgctcgc gcgatcgagg cctccgccca ggcctcgtag cccgggatcc ggcccggccg
     6901 ctgctcgcgc gacgccgtgg cttgaatgag tgcgcggcgc tgctcggcgt aagagcgcga
     6961 aagcagcgtc tcgacgggga tctcggtgtg ggcgggatcg ccgtagtagg cttcccggtc
     7021 cgccatcgca agcttcagag tctcggtcac gaggtggacg aagtcggcgc ctgccggatc
     7081 gacttcgccg atatttgtcc cctccaaaat gcgcaacgac tgcagcaaga gtggtgcttg
     7141 cgaccagaca ccggccttcc acaccgtcca gccgttgtag tcgacggcga gcggggcctc
     7201 tgtcgtggcc tgccacgcag ccatgtcgtc ggcggtcagc acgcccttgc gccggccccc
     7261 ggcggcgtcc atcacctccg ccgtctccac ccagcgcccg atcgcctcgg cgaggaagcc
     7321 gcggtagaag gccgcccgtg ccgcctcgat ctgctcctcg cgcccggaac gcgtctctgc
     7381 ctccgcgagc aggcgacgcc acgtcgccgc cagcgccgga ttgcgaaaga gctggccggg
     7441 ggcgggcggc gctccgcccg gcagccagac ttcggctgac gtcggccatt ccgccttgaa
     7501 gaaatcggca agccctgcga tcgcgtccgc ggcgcgggcg aggatcggat ggcccgcctc
     7561 ggcatagtag atcgcgggct cgagcacgtc gcgcaggcgc atcgtgccgt agtcgcgcag
     7621 cagcagcatc caagcatcga aggcgcccgg cacgacggtt gcaagcatgc cggagcccgg
     7681 cacctcgctc agcccctgtg cccggtagtg ggcgatcgtt gctttcgcag gcgcgacgcc
     7741 ctggccgcag atcaccgtcg gcgcctcggc atccgcgggg cgcacgagca gcggaacttc
     7801 gccgagcggg ccgttgaggt gcggctcgac gacgttcagg acgaagccca tcgccacggc
     7861 agcatcggct gcccggccgc ccttctcgag aacgctcatg ccgacggcgg aagcgagcca
     7921 atgggtcgac gttgcgacac cgaaggtgcc cgtaatctcg ggccgcgtcg tgaaggtcat
     7981 acggggctcc atgaactaag ctctcatctt tcgcgcggat cgagtgcgtc gcgcaagccg
     8041 tcgccgagca ggttgaagcc gagcacgacg aggaagatcg cgattcccgg tgacagcgac
     8101 atccacggcg cctgctcgag gaagttcttg gcagtgttca gcatcgagcc ccacgacggc
     8161 gccggcggct gttgcccgag gccgaggaac gacaggctcg cctcggcgat gatggcagtg
     8221 gcgacggtga gcgtcgcctg gacgatcagc ggtgcggtga tgtttggagc gacgtggcgg
     8281 gccaggatga gccagcgcgg cacgccgacc gcgcgcgcgc cttcgacgta ttcctcggcc
     8341 ttgacggcca gcacctggcc gcgcgtcagg cgcacgaaga tcggcacctg ggacaatccg
     8401 atggcgatca tcgcgttggt gagcgatggc cccagcgccg cggccagcgc aatggcgagg
     8461 atcaggaacg ggaccgcgag aagcgcatcg gtggcgcggc cgatgacggc atcgatccag
     8521 cccccgaacc agccggcgac gaggccgatg ggcataccga tggcgagcgc gatggcgacg
     8581 gacacgacgc ctgcatacag cgacgcgaca ccgccgtaca tcacgcgcga gaggatatcg
     8641 cggccgagct cgtcggtgcc gagccagtag agcgccgacg gcgccttgcg cagcgccgcg
     8701 aaattggtct tggttggatc gtagggcgcg atcagcggag ccagcaccgc catcaacaca
     8761 aacgcggcga ccagcgcggc gccgaagaca gcggtgcgat tcctgagaaa cttcgcgagc
     8821 gcgcgccgct cctgcggggc gatctcctcg cccgccggca tgcgctcggc atctgtcccg
     8881 gcgatggccg tcacgtccta agcctcggat tgatcacgat gtagaggaca tcggcgacga
     8941 ggttcatcag gatgaacgcg atcgccgtgc acagcacgac accttgcacc accccgtaat
     9001 cgcggttgaa gacagcatcg acgatcagct tgccgaagcc cggaatcgtg aacacctgct
     9061 cggtgagcac cgctccggcc agaagctggc cgaagagcag cgtcgcaagc gtcacgatcg
     9121 gcacgagcgc gttgcggaag gcatgtttca ggatcacgac gcgcggcagc aggcccttgg
     9181 cccgcgcggt ccggatgtag tcgaggctca ggacctcgag catcgccgag cgggtgtggc
     9241 gcatcaggac ggcggcgaga ccggagccga gcacgaacgc cggcatgatt gtcgtctgaa
     9301 aggcgcgggc ggggtcctcg aacacggaca cgaaccccga ggcgggcagc cattgcaggt
     9361 tcaccgcgac gacgagtatg aggatgatgc cgagccagaa gttcgggatc gagagccccg
     9421 acagcgagac catgctggtg accgcatcga tccaagttcc cttgcgcaca gcagacagaa
     9481 tgcccatcgg gatgccgatg gcgagcgcga tcacgatg
//
LOCUS       DASVDU010000127         2675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_843468, whole genome shotgun sequence.
ACCESSION   DASVDU010000127 DASVDU010000000
VERSION     DASVDU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843468"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF226_08060"
     CDS             <1..220
                     /locus_tag="VF226_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073979.1"
                     /translation="AKLIVDIAAGAVEDKPEQPERKTNPAAVERGKLGGKNGGKARAE
                     AVTLKKRQEIARKAAAARWKGARKKVQT"
     gene            complement(428..1312)
                     /locus_tag="VF226_08065"
     CDS             complement(428..1312)
                     /locus_tag="VF226_08065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7073980.1"
                     /translation="MREQALESRPSGASADAISYHYDVGSEFYRLWLDRNLTYSAARW
                     GDPRQVAISPVSLELAQEAKLDFHLRAVGVGPGDSLLDVGCGWGSMLRRAVEQYGVAA
                     ATGLTLSTDQYNHLRALALPGVEVRLENYEDFRPAGKFSGIVSVGAFEHFTRPGLSAT
                     EKVAIYRRFFELCHGWLDRGARLSLQCITWGNVPRDRTGFIMAQDVFPETDPPYVADV
                     LEASADTFEPIYMENCRNDYVRTLQVWLERLRARRREIESMTRAGTFDFYERYLRRSI
                     YGFKKKQFQLCRFVFQRH"
     gene            1926..2147
                     /locus_tag="VF226_08070"
                     /pseudo
     CDS             1926..2147
                     /locus_tag="VF226_08070"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
     gene            2138..2395
                     /locus_tag="VF226_08075"
     CDS             2138..2395
                     /locus_tag="VF226_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7073981.1"
                     /translation="MSLSRDVTGGHMEMLADQIRPLLKVVETELNKHAVKPKEDTPGS
                     LLLEVTTNFLLGEAGGRSRVIGLLEEMIESVRHGTVIERRY"
BASE COUNT          516 a          794 c          888 g          477 t
ORIGIN      
        1 cgccaagctg atcgtggaca ttgccgcagg cgcggttgag gacaagcccg agcagcctga
       61 gcggaagacg aatcctgccg ccgtggaacg ggggaagctt ggcgggaaga acggcggcaa
      121 agctagggct gaggctgtta ctctgaagaa acgtcaggag attgctcgaa aagcggcagc
      181 ggcgcgctgg aagggggcgc gtaagaaggt tcagacataa gcggcagcat cagaatgtca
      241 ccccgctttg gatgcgccac atccaagcag gattccgttg gcggtcacac gttggcagga
      301 ttggcgcagt gcaatccaca aacattttat ggtcatcccc gcgtaagtgg ggatccagac
      361 ggcccaatcc gtcccgtgga ttcccgcttc tgcggggatc acgagatcag gtttacagtc
      421 gaggtcctca atgccgctgg aagacgaagc ggcagagctg gaactgtttc ttcttgaagc
      481 cgtagatcga ccggcgcagg tagcgctcgt agaagtcgaa ggtcccggcc ctggtcatcg
      541 actcgatctc ccgccgccgg gcgcgcagcc gctccagcca gacctggagc gtgcggacgt
      601 agtcgttgcg gcagttctcc atgtagattg gctcgaaggt gtcggccgag gcctccagca
      661 cgtcggcgac atagggcggg tcagtctcgg ggaagacgtc ctgcgccatg atgaagccgg
      721 tcctgtcgcg cgggacgttg ccccaggtga tgcactgcag cgaaaggcgc gcgccgcggt
      781 ccagccagcc gtggcacagc tcgaagaacc gccggtagat cgcgaccttc tcggtcgccg
      841 acaggccggg gcgggtgaag tgctcgaagg ccccgaccga gacgatgccc gagaacttgc
      901 ccgccggccg aaagtcctcg taattctcca gccggacctc gacgcccggc agggcgagcg
      961 cgcgcaggtg gttgtactgg tcggtgctca gggtcaggcc ggtcgccgcg gcgacgccgt
     1021 attgctcgac cgcccggcgc aacatcgagc cccagccgca gccgacgtcg agcaggctgt
     1081 cgcccggccc gacgccaacg gccctcaggt ggaagtcgag cttggcctcc tgcgcgagtt
     1141 ccaggctgac cgggctgatc gcgacttggc gcggatcgcc ccagcgggcg gcgctgtagg
     1201 tcaggttgcg gtcgagccac agccggtaga attcgctgcc gacgtcgtaa tggtaggaaa
     1261 tggcatcggc cgacgcgccg gacggtcggc tttcgagggc ttgctcgcgc atggtgtcaa
     1321 aacctcgacg cagtgcggat gccggtgcga ggccgttggc cggcctccgc ggttcctccg
     1381 gatggcagtc taatcagcga ttcgcaggct gtcacgaatg ggttttggct ctctctcggt
     1441 tcatgttgac catgctccga tggatggctg tttcgagatg atcggcatcg cacacgattt
     1501 cgatggacga ggcgatgacg cttgcgatta tgccaggcgc gttcgatatc gttcaactcg
     1561 ggcgcatact ttggcagcaa ctcgacagtg agctaggggc gcggcgagcg cctttctgct
     1621 cggttttctg gtgtggatcg gaccgttgtc gagaacgaca accacgggcc ggaccggctc
     1681 gccgcgtcgg gacccgtagc gggtttcgat ctcgtccaag agcaccacgc gccaccatcc
     1741 actgtcgccc tggagagcgg ccgtgccgag gatagggagg cgctatggcg ggccgatcgg
     1801 acgccggaac cctggccccg caggcgggtt gactcttttg gtgtttgcgt tctagccgca
     1861 ggaggtgaga tggcaagggc aaacccgagg cctgagaacc tcatccgggc aaggaggtcg
     1921 aggacacgcg tgtgagcaat ccggcaggcg agaacctcgg caagatcgag gatgtcgtcc
     1981 tcgacaaggt caaggggcag gtgacctatg cggtgctctc gttcggtggg ttcctccgca
     2041 tgggcgacaa gccgctgaga agggcactga tcgggcccat gatggacatg atcgcgccgc
     2101 cgaaacggtt gcaagccaaa ccaaggagga gcgctagatg tcattgagca gggacgtcac
     2161 cggaggtcat atggagatgc ttgcggacca gatccgcccc ctcctcaaag tcgtcgaaac
     2221 cgaactcaac aagcatgccg ttaaaccgaa agaggacact ccgggatcgc tactgcttga
     2281 ggttacgacg aactttcttc tgggcgaggc aggcggtcgc agcagggtca taggtttgct
     2341 agaggaaatg atcgagtctg ttcgccatgg caccgtcata gaaagaagat actgattgcc
     2401 acgcattccc atcgttcacg gacaactgag ccgcctccgc tccggacggc agcttccggc
     2461 gcatgatcgt ccgcggctgc catatccgtc tcataccata tccgtctcat accggcgcac
     2521 ccctgtaacc gccgcagctg ggcgaacgcg cattacgcgt cctcgtctgt agtaggtaag
     2581 cacggcgaat aaccgcttct ggtacgacgg cgacctggct gaccaatagc tcattcgccg
     2641 gcattcgggc ctattgcggg cgctcggcgg ctaaa
//
LOCUS       DASVDU010000128        23093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_989224, whole genome shotgun sequence.
ACCESSION   DASVDU010000128 DASVDU010000000
VERSION     DASVDU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23093
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989224"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VF226_08080"
     CDS             complement(<1..457)
                     /locus_tag="VF226_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX7073982.1"
                     /translation="MPALAHHMMGGQVPQTFAQGLLSGIGHPLIGIDHFAFVVAVGVA
                     AVLAGKLWALTAAFVIGAFAGCLIHLAGFTLPMAELVIAASVLLLGAIIASNREVSPM
                     ALGILFVGAGLFHGWAYGESIFGAERTPLIAYLAGFSLTQLAVAILAGPV"
     gene            complement(945..1703)
                     /locus_tag="VF226_08085"
     CDS             complement(945..1703)
                     /locus_tag="VF226_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7073983.1"
                     /translation="MTRSASTAAVLRGAILAGEFAPGERIYEQGLSERLKVSRTPIRE
                     ALKSLAGEGLLDHVPNRGYYVRAYSVDDIVQAYEIRAALEGLAARRAAQLGLADEQRA
                     IIEKALEDGEAFLKKGNFSPDDRVLYGEINAAIHSAILAGSQSRMLRDQLRLSQQIAP
                     SSHRNIVAFERRDVRRRHDDHYRIYEAILCRDGARAELLMRDHVESVKIALIRSISRD
                     LPLPEQAGGRLAFESGGPVGGRRRRDVREPDSSR"
     gene            2029..2721
                     /locus_tag="VF226_08090"
     CDS             2029..2721
                     /locus_tag="VF226_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7073984.1"
                     /translation="MNLLFTPNIVTLPIVGRHERFPVRRVYCVGRNYLEHIREMKEAD
                     ERDPPFFFQKPTDAVTSEGEIPYPPETGDFQFEVELVVAIGAAAINIPVERALDVVFG
                     YAVGVDLTRRDRQRESFAKGLPWEIGKSFDKSAPCGPIHPVAAVGHRSTGTISIEVNG
                     VERQRGDLRQMIWSTPEIIAKLSASYRLEPGDLIMTGTPAGVGPLEPGDRLVALIEGL
                     SPFTFSIGAKVT"
     gene            2725..3615
                     /locus_tag="VF226_08095"
     CDS             2725..3615
                     /locus_tag="VF226_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7073985.1"
                     /translation="MLPTERISYSAITERAPLKLPGNARMVAWVIVNIEEWDPRETMP
                     RTVLTPPAGGSPMPDIPNWAWHEYGNRVGFWRLLEALDEHAIKAVLAINGKAIKTYEA
                     ISKAALGRGWEFMGHGFGQKNMQKVPDEREDIRMTVEAVKAFTGKAPRGWLGPGLTET
                     WETPDLLVEEGFDYVCDWVLDDQPVVLKTRTKPILNVPYTQECNDVAMMLIQHHKASE
                     YYDRAMDQFEQIYQDSAKSARVMALVVHPYIMGAPHRAKYFRRVLAEIRKRPDVIFWT
                     GEEICDWYLGERPDALKNVS"
     gene            3619..4479
                     /locus_tag="VF226_08100"
     CDS             3619..4479
                     /locus_tag="VF226_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073986.1"
                     /translation="MASLNLVQVLNGLTFAALLFIVASGFTLVFGLLRIVNLAHGALY
                     LFGGYIGYTVIATTGNFVLGVLAAMLAIACIGLFVDQTLLRFVRGFELREVLLTLGVA
                     FVLNDLALVIWGGDTFSVQVPEFLRGAVRFSGIFYPKYRLFVLVLGVLTFVGLWLLMN
                     RTRLGALIRAGVDDAEMVEAMGINIRRVFLATSMLGASLAGLGGTIGGAFLTLYPTAD
                     SEILVFSLAIVIIGGSGSLVGAAVGALLVGMLNTIGQVLFPELAYFVIFGPMAVLLAF
                     RPLGLFGRAA"
     gene            4479..5438
                     /locus_tag="VF226_08105"
     CDS             4479..5438
                     /locus_tag="VF226_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7073987.1"
                     /translation="MASAITSVRHSPLAFIVVLAAIFGALPPLVSTYELSLANEVLIF
                     ALLVMSIDVLAGYAGRVSLGHGAIFGISTYVVIYWTTIMGGSPWVGCFLGILAATFAA
                     GVFALLAARVGGVYFLLLTLALGMIVWGVCLRWTSVTGGENGIRGSGRPDIIADPTVF
                     YYVTLAVVLALTVIIWRFVHSPFGLTLRGIRDSESRMRSLGYGAATHIFLAFTITGLF
                     AGVAGALYALFNDFVSPSTVQLAQSVEGLLMAIIGGIGTLFGSFIGAAAIILLENFVS
                     QYTERWQMILGLTFIGAMIFAPEGILGALRKLFARSSSRQTKS"
     gene            5467..6732
                     /locus_tag="VF226_08110"
     CDS             5467..6732
                     /locus_tag="VF226_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7073988.1"
                     /translation="MDRRTFLKHSGLALAATATSAFPMPHIARAQAGKAIRIGLMAPL
                     SGVVAAGGKEIVEGFNMYWEEKGNQIAGRPVEVVVEDDAGNPDTALQKARRLVEQENV
                     DFLFGNLLANTGLAVANYVRGNGVPYFIPVIAADDLTQRQRVKNVIRVAGYTASQMPR
                     PLADWALKQGYKRIATISQDYTFGHEQCAGFSQVFTEGGGTIVGQLWNPLNTADFSPY
                     IGQLGSMNIDAIFTMELGADASRFMKQYSDFGLKGQIPLLGAQNFTDQSVIRTSGPEC
                     EGIVSSAHFAEGSDNPETQKFVKAYSEKYGKLPSLYGFSHYSGALWVGQVIEGLKGDI
                     SNRDKFLDAVLNSELTNSPLGRPVRFDDYGNPIYDVYIRKVVKNAEGKYWNVPIETYP
                     SVSQFWTYDPKEYLAKPPFSRDNQGIKKT"
     gene            6801..7559
                     /locus_tag="VF226_08115"
     CDS             6801..7559
                     /locus_tag="VF226_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073989.1"
                     /translation="MSETALVLENIVVAFGALKAVDDVSISVRRGERRAIIGPNGAGK
                     TTLFNAITGVFPPTSGRVVFEGQNVTRMPAQKRARMGMSRTFQITNLFPTMTVEENMQ
                     LALRGTSRSKFSMLGSTRLTSEEQDRVASALRVSRLEERVSVAVNELSYGEQRQLEMA
                     MALISKPRLLLLDEPAAGLSPAERLMVAEIIRSLPQDLTMVLIEHDMELVLNLVDWVT
                     CLNNGRLLAEGSPEDIRSNQHVQDVYLGKARSDA"
     gene            7552..8256
                     /locus_tag="VF226_08120"
     CDS             7552..8256
                     /locus_tag="VF226_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7073990.1"
                     /translation="MLEVRNLHSFYGDAHVLHGVSLEVRPGEVVALLGRNGMGKTTLI
                     RSIMRLAQPQVREGSLLWKGEELRNLAPHEVALRRIALVPQGRRLFSSLTVTEHLTML
                     KSASSKQGWTVDRVFEMFPRLAERKNHRGNQLSGGERQMLAIGRSLMIDPELVLMDEP
                     SEGLAPVLVQQLETIIADLGKTGLAILLVEQNLYSALSVASRFYILETGRVVHEATAD
                     EIKKDQAPLMRFLGVH"
     gene            8259..9749
                     /locus_tag="VF226_08125"
     CDS             8259..9749
                     /locus_tag="VF226_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7073991.1"
                     /translation="MKRWDLFIGGEYRPAESGRTFTVENPKDGSIIAEVAEGDAQDIE
                     AAVAAAEHAFPQWSALSGAERGDIMHRIAEILRRRLPELATIEIDQIGRPRREMTAQL
                     ARLPEWFSYFGAVARTHEDTVPPFSGDYLNYTHRVPLGVVGHITPWNHPLLILTKKVA
                     PSLAAGNAMVVKPSELAPITPLLLGEILKEAGVPDGTYNVVPGFGAAAGLALSSHSRI
                     RKLDLTGGTETGKAVASIAGGNLVSVSAELGGKAAVIAFPDSDVGRVVAATMFAAFIA
                     SGQTCVQGSRLLVHRSIHDEVVSELVRRTREIRLGDPQLETTQMGPLVSSRQRERVEE
                     YVRIGIEEGAHLAYGGVRPECPSLAAGYYFLPTIFTNVSPQMRIAQEEIFGPVVCVIP
                     FETEQEAIAIANGTEFGLATSIWTRDIARAHRVAHSLHCGITWINDHHRIDPASPWGG
                     FKMSGIGRENGIVAYEAYTQIQNIIVNLSDSTFDWYAEDGQQKRYS"
     gene            complement(9796..10803)
                     /locus_tag="VF226_08130"
     CDS             complement(9796..10803)
                     /locus_tag="VF226_08130"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7073992.1"
                     /translation="MTSSRIPPRIDIHAHYYSERYLQLIEDAGCSCGACVERDPRGPV
                     VKAGPLHAGPLAPRFIDLDLRIADMDAQGVSAQALSLTQPMVYWAEPSLARRLSAAFN
                     DDLVRAHERYPSRLFGLAMLPVHHAGEALAELERIRQAPGIRGIYMGTCIGDMDLSDQ
                     SLFPVYEAIEAAGLPIFLHPLKVIGMQDRLKPYFLYNLLGNPFDTAVAAAHLIFGGIL
                     DRFPKLEFVLPHAGGALPFLIGRLQHGWTVRPELAHTQQGPETYLRRFYYDTIAHSDD
                     ALAYLIKTVGSDRVLLGSDYCFDMGYERPVQVVERQAQLAPSEQNAIFSENAANLLKL
                     R"
     gene            11075..11968
                     /locus_tag="VF226_08135"
     CDS             11075..11968
                     /locus_tag="VF226_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7073993.1"
                     /translation="MTEQHIDPNLIRSRLPYSPITQRPPLRLPKGERVAVWTIVNVEN
                     WSPDNAMPRTVLPPPMGQPLLPDIPNWAWHEYGMRVGFWRFLEVLGARNLKASFAVNG
                     SACRVYEAACKAAHEAGWDFIGHGFIQRPMHRVADQRAAIADTIEAIRSLTGKAPRGW
                     ESPGLTETVETLDLLAEAGIEFVCDWCLDDQPVRMNTKAGPIVSIPYTVEVNDVVMTA
                     VQGHRSDEIYKRGVDQFDRLYLDGARTPRVMAISIHPFLTGVPHRIKYLEMLYDYILG
                     HEGVAMMTGSEILDWYVAQTS"
     gene            complement(12046..12654)
                     /locus_tag="VF226_08140"
     CDS             complement(12046..12654)
                     /locus_tag="VF226_08140"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7073994.1"
                     /translation="MRWPWPAPVDDGSADHLVCGLGLPDIPLPATSGRMVSLARVPGR
                     AVVFCYPWTGRPGVPNPPDWDTIPGAHGSTPEAEGFRDLYPGFAEMPTAVFGLSLQPT
                     EEQQEFASRLALPFELLSDAAQAFQKALDLPVFETGGARYLKRLTLFLQDGRIEQVFY
                     PVHPPNTHAREVLAYITADISYEEEARLRSELPESNSSKTIR"
     gene            complement(12651..13244)
                     /locus_tag="VF226_08145"
     CDS             complement(12651..13244)
                     /locus_tag="VF226_08145"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7073995.1"
                     /translation="MSTRNPDLHTVDWSTIPAPTDDGAARHLEGMRVPDVTLPATDGS
                     SVCLSCLTGLTVVYAYPRTGRPGEISLVENWDMIPGARGCTPQSCAFRDHYQDLVAAG
                     AAHVFGLSTQDTAYQREMADRLHLPFPVLSDEDLALTRALKLPTMEVAGMTLLKRLAM
                     VIADGEIAKVFYPVFPPDRNAGDVLAWLQRYQMAGQA"
     gene            complement(13257..14027)
                     /locus_tag="VF226_08150"
     CDS             complement(13257..14027)
                     /locus_tag="VF226_08150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7073996.1"
                     /translation="MDASADVCLALTRDDLLAIERAAVHAWPASETQNIAGWLWRYSG
                     GGSQRANSVSTLAFDGADTIAAIEEAEELYFCRGAVSRFQVGTELAAPADLDLQLERR
                     GYRLEEPVTTLARRLGQPSWPAGIEITAQPTAGWMEVYLSNIAPNRHAAAPAILDAVP
                     APRAFLSYVSGGQVVSTALAVLHDHIVIAECVGTRAEARRTGGARKVMAALEAWGSAR
                     GARIAALQAVAANEPAQRFYAALGYRKVNGYHYRVRDC"
     gene            complement(14164..14640)
                     /gene="smpB"
                     /locus_tag="VF226_08155"
     CDS             complement(14164..14640)
                     /gene="smpB"
                     /locus_tag="VF226_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7073997.1"
                     /translation="MAAKTNGGRRLVAENRKARHEYFITDTYEAGIALTGTEVKSLRQ
                     GQANISESYAAMEDGGLALINAYIPEYKQAGEFFNHEPRRPRRLLMHRRELHKIANAI
                     SRQGMTLVPLELYFNERGIAKLKLALAQGKKLHDKRQTEAKRDWERQKARLLREKG"
     gene            complement(14667..15557)
                     /gene="dapA"
                     /locus_tag="VF226_08160"
     CDS             complement(14667..15557)
                     /gene="dapA"
                     /locus_tag="VF226_08160"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7073998.1"
                     /translation="MFKGSFTALISPFKNGAFDTSAYERLIEWQIAQGTHGLVPVGTT
                     GESPTLSHEEHKHAVEVCVETARKRVPVIAGAGSNSTAEAIDFTRHAKDVGADAVLIV
                     MPYYNKPTQEGLYLHVKAINDAVDIPIVLYNVPSRTVADMSVATMARCAQLKNVIGVK
                     DATANLSRASLQRLACGPGFVMLSGEDATALGFNAHGGQGCISVTSNVAPALCAEFQN
                     ACLRGDFATALSIQDRLMPLHDALFIETSPGPVKYAASLLGLCSAETRLPLAPISEAS
                     KRAVESAMVGAGLLQAKAAE"
     gene            15853..18051
                     /locus_tag="VF226_08165"
     CDS             15853..18051
                     /locus_tag="VF226_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX7073999.1"
                     /translation="MPIRVPALQGLLTASAIAAMAAFWPATAANPKLVPAPSAAPVVK
                     ANIGRFPAKPLLTQDHAAREADHIARFDAAIAAARGHELSEDDAKRINDAIGAYYARD
                     IEKARAIQNDITDPIGRKLVEWYRLRAGLAEPFEIQAFLDANPAWPDRDRLRANFEKL
                     LFSKGGNAQVIKEAFAKSEPTTGLGFAALASAYLVLGDKERAKALAAYAWREESIPEE
                     FEAGFLERFGGLLTEADHKWRFDRLLISDPRWSSQRKARAAVAKRVIPLLGKAEQKKA
                     EARLAAFLRRKSAAKQLSALPADSSADWGLAFQRADLFLSQGKDEQAWKILLSAPTDP
                     KEIVNPDGWWELRREAAYEALHAGKPKVAFELVRDAGPLSVNPRNEQTFFAGWLALRH
                     LNQPKTALPFFEANKDGSDGPLSRSRALYWLGRTHKALGENKKAREEFAAAAAYIDTF
                     HGQLAREELGDDRPELVITAPEEPTDEQIARFNRLDAVRAAVIAAKAGINRTLTRAFL
                     SELQHYFGAEAEVAMVAHLAQALGDNQMAVRIGKAAIARGMNLVHYSYPIHAFPAYEP
                     LRTPPEPALLLALARQESEFNSSIMSGAGARGILQVMPGTARHICRSYKIKYNVRKLM
                     ADPSYNAMLASAYVGDRMDDVDGSYILTLTSYNAGPGRTRQWIREFGDPRDPGVDPID
                     WIYQIPFEETRDYVQKVLSNVQIYRARLGKTNGSIVADLTRARRKPQEAP"
     gene            18084..18989
                     /locus_tag="VF226_08170"
     CDS             18084..18989
                     /locus_tag="VF226_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7074000.1"
                     /translation="MANEPEPWKDIFFSARDGLRLYARHYPAPGSRLRPVLCLAGLTR
                     NSRDFHALAVALSTGEAARDVYTLDYRGRGRSAYDTDWRNYSVAVEMLDVQDLVTLAG
                     LEKPAIIGTSRGGLITMALAAAQPGAIGAVVLNDIGPVIERDGLTRIAGYVGNTPLPT
                     SWEDAARLVAELNRRAFVAVPEEDWAEIARQWFNDDKGYPAPGYDPALAKSFSSADGP
                     IPDLWPQFAGLSRVPLLVIRGENSDILSETTVQQMRTRHPRCATLTVPGQGHAPLLRD
                     ASTIAAIAAFLREADAVVLSSAQAA"
     gene            complement(19042..19923)
                     /locus_tag="VF226_08175"
     CDS             complement(19042..19923)
                     /locus_tag="VF226_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX7074001.1"
                     /translation="MFSRTNRLQYNARPERPDPAYARKLQEVLGGQWGEISVMMSYLF
                     QGWNCRGPAKYRDMILDIGTEEIGHVEMLATMIARLLESSPIEMREEAAKNSAVGAVL
                     GGARVEDVLMAAMNPQHVIVSGAGATPTDSVGYPWNARYTISSGNMLADFRYNVTAES
                     QGRLQVVRLYELTSDPGVRDMLSFLIARDTMHQNQWLAAIRELEEDGLESTPCPSSFP
                     QENEFRHVSYQFLNFSEGTESQDGSWASGPSPDGRGQIDYIGMPGSEGHDDGELGPVD
                     PRIHGTPAQPVTATVSP"
     gene            20145..20426
                     /locus_tag="VF226_08180"
     CDS             20145..20426
                     /locus_tag="VF226_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074002.1"
                     /translation="MSDKTVLAVPGRKLSVPRVFKRTLQISMAALLTSAAALTFIVAN
                     SMLLGRGGPLQAISAWLAFIKRPDIQATIILTAVVTVLFVYWQRDRERR"
     gene            complement(20380..20898)
                     /locus_tag="VF226_08185"
     CDS             complement(20380..20898)
                     /locus_tag="VF226_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7074003.1"
                     /translation="MAPLTRPVTIIGKAAGARTGVVDLHFNARKPHQSSLYRRTMHDG
                     TVRRAEMDTLDNMLADHPGQRLVVKIDVEGAELEVLEGATRTMQRTGCMFVEVNMGYL
                     FERERNPLPDVDSLMKGAGLTLYRIMDATMVGSAADVQITKADFCYVSNGLLAERFTS
                     ARGHAASRRKAR"
     gene            21535..22326
                     /locus_tag="VF226_08190"
     CDS             21535..22326
                     /locus_tag="VF226_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7074004.1"
                     /translation="MELTTSPAVAIERRGAIAIITINNPERMNALSASVRDALRLAFE
                     GFEDDRSVRAVVLRGSGNRAFASGGDISEFPRMDSAAARSNFEKLEALFAAIERLHLP
                     VIAMIHGYALGGGCELALACDLRIAASDSKFGIPIGRLGHTLDLASARRLVRLIGAPK
                     VKMLLWTDEIIDAGEARRVGLIDEVVSPDELESYTLSLASKIAEKAPLSIAGTKSVLA
                     HVTKHPVARPGEDIAQFAAPLFETADFKEGVRAFLEKRKPTFVGQ"
     gene            22323..>23093
                     /locus_tag="VF226_08195"
     CDS             22323..>23093
                     /locus_tag="VF226_08195"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7074005.1"
                     /translation="MTHSPLQGIRVIDFTHMRAGPQCTMMLGDMGAEIIKIESPRGDG
                     TRLYGRDINGEALDFLSVNRNKKSVVLDLKSPEGRQAAMDLISTADVLVENFRIGVME
                     RLGFGRERLAELFPRLIYCSITAYGQTGPYAGLPGYDQIAQGMSGLMSLTGTKESGPL
                     RVGLPIGDLLAGAFAAYGIVLALFERQHSGRGQFVHTSLLRALVSLLSFQAARYLMTG
                     EVPDVVGNHHPIVVPNGTFATSDGLINIGCSTQEQWESL"
BASE COUNT         4343 a         7201 c         7085 g         4464 t
ORIGIN      
        1 cgacggggcc ggcgaggatc gcgactgcga gctgggtcaa cgagaaccct gcgagatacg
       61 cgatcagcgg cgtccgttct gcgccgaaga tcgactcgcc ataggcccaa ccatgaaaga
      121 gcccggcgcc gacaaacagg atgccgagcg ccatcggaga aacctcgcga ttgctggcga
      181 tgatggcgcc gagcaaaagc accgacgccg caatgaccag ctcagccatg ggcagcgtga
      241 acccggcaag atggatgaga cagcctgcga aagcgccgat gacgaaggca gcggtcaacg
      301 cccacagctt gccggcgagc acagccgcga caccgacagc gaccacgaag gcgaaatgat
      361 caatgccgat cagagggtgc ccgatgccag atagcagtcc ctgcgcgaac gtttgcggca
      421 cctggccgcc catcatgtga tgggcaaggg cgggcatcgc gctcgccgac catgcaagag
      481 ctcccaaacc agcaaggcgt gatacatgag gtgaggaaag cggcatggta cccccagttc
      541 aactagcgga agggccaccg accgccaatc gccatgcggc gtcgtcaatc gcggccgaat
      601 gcccccgttc gttcacgtcg atctttacga cttgcggcct cctggctggc ggaccgacgt
      661 agcctccagc ttccagaccg agctggcagc cggcggcaac cctgttgcga gcgcctcact
      721 cgcaatggcg gggcaacggc agctgcaatc ctcggtttgg atccgttgtt gcgcgttccc
      781 ttttcatctc cgtctggaga acggacgcgc gagacggtag acggagcctg ctagtgcgtc
      841 aatcgcatcg aggtctgacg agtgggcggc ccacgtggag gacgccgaaa gttcgtggca
      901 ctgagactcc cgcggggctg aataggtcga tcttacaaag gattctagcg tgaagagtca
      961 ggctccctga catctcttct tcgacggcca ccgaccggcc cgcccgattc gaaagccaat
     1021 ctccctccgg cttgctccgg taacggcagg tcgcgcgaga tggaccggat cagcgcgatc
     1081 ttcacgctct cgacatgatc gcgcatcagc agctcggccc gggcgccgtc acggcacagg
     1141 atcgcctcgt aaatccggta atgatcatcg tggcggcggc gcacatcgcg gcgctcgaac
     1201 gcgacgatgt tgcgatgcga agacggtgca atctgctgcg acaggcgcag ctggtcccgc
     1261 agcatgcgcg attggctccc cgcaaggatg gcgctgtgga tggcggcgtt gatctctcca
     1321 taaaggacgc gatcatccgg ggagaaattg cccttcttca aaaaggcctc gccatcctcg
     1381 agagcctttt cgatgatcgc ccgctgctcg tccgccaacc ctagctgggc ggcacgcctt
     1441 gccgccaacc cttccagcgc ggcacgaatt tcataggctt ggacgatgtc gtcgacggag
     1501 tatgcgcgga cataatagcc gcgattgggg acgtgatcca aaaggccctc gcctgctaga
     1561 gacttgagcg cttcacggat cggtgtacgg gataccttca gccgttccga cagaccctgc
     1621 tcgtagatgc gttccccagg agcgaactcg cccgcgagaa tcgcaccccg caggactgca
     1681 gccgtcgatg cactgcgagt catgagctcg gttttcttgc gagctgaatt cagcttagct
     1741 ctcgaattca cacttgcaca cgattcccaa tctgaacatt gccgcttcgt tccgcatcat
     1801 tggcaatgag atgccccggc atttgagcat ttcgcgtgga aatagctgcc tctcccagca
     1861 cacctcaacg ggactcgaaa cagctaacat gtatacattt gtccttgaca gaaggcgaga
     1921 tgcatatagc attttcaatg ccgatgctgg aggtggggcg atgttgaagc atgacttcga
     1981 ggggaagcgt ccttggcctg cgatccggcc gctggagaca gtcggacgat gaatttgtta
     2041 ttcaccccga acatcgtcac gctgcccatt gtcggccgcc acgagcggtt tccagtacgg
     2101 cgcgtctatt gcgtcggccg caactatctc gaacacattc gcgagatgaa ggaagccgac
     2161 gagcgcgatc cgccattctt ctttcagaag ccgaccgatg ccgtcacttc ggaaggagaa
     2221 atcccttacc cgcctgagac cggagatttt cagttcgagg ttgagctggt ggtggcgatt
     2281 ggcgctgcgg ccatcaatat tccggtggag cgcgctctcg atgtcgtatt cggctatgcg
     2341 gtcggggtgg acctgacgcg gcgtgaccgc cagcgggaat cgttcgcaaa gggattgccg
     2401 tgggaaatcg gcaaatcgtt cgacaaatct gccccctgcg gtcccattca ccctgtggcg
     2461 gccgtcggcc atcgctcaac cggcacgatt tcgatcgaag tcaacggggt ggaacgccag
     2521 cgaggcgatc tcaggcagat gatctggtcc acgcctgaga tcatcgcgaa actgtcggcc
     2581 tcctaccgac tcgagccggg cgatctcatc atgaccggca cgcctgccgg tgtcgggcca
     2641 cttgaaccag gcgacagact cgtcgcgttg atcgaagggc tttctccctt cacgttctcc
     2701 attggtgcga aggtaacctg acaaatgctg ccgaccgagc gcatttctta ttccgcgatc
     2761 accgagcgag cgcctttgaa gcttccgggc aatgcccgca tggtcgcctg ggtgatcgtg
     2821 aacatcgagg aatgggatcc gcgcgagacc atgccgcgca cggtgctgac tccaccggcg
     2881 ggcggatcgc cgatgccgga catcccgaac tgggcgtggc acgagtacgg caaccgtgtc
     2941 ggtttctggc gtcttctgga ggctctggac gagcacgcaa tcaaagcggt tctggcgatc
     3001 aacggcaagg cgatcaagac ctacgaagcc atttcaaagg ctgcactggg gcgcggctgg
     3061 gagttcatgg gccacggctt cggccagaaa aacatgcaga aagtacccga cgaacgcgag
     3121 gacattcgca tgacggtaga agccgtcaaa gcgttcactg gcaaagcccc acggggctgg
     3181 ctcggacctg ggctgacgga aacctgggaa acgccggatc tcctggtcga ggaaggattc
     3241 gactacgttt gcgattgggt tctcgacgat cagccggtcg tgctcaagac tcgcaccaag
     3301 ccgatcttga atgttcccta tacccaggaa tgtaacgacg tggccatgat gctgatccag
     3361 catcacaagg cgagcgaata ttacgaccgt gccatggacc aattcgagca gatttatcaa
     3421 gacagcgcca aatctgcacg cgtcatggca cttgtggtgc acccctacat catgggcgct
     3481 ccgcaccgag ccaaatattt ccgcagagtt ctcgcggaaa tcagaaagcg cccggatgtc
     3541 attttctgga ctggcgagga gatctgcgac tggtacctcg gcgagcggcc ggatgccctg
     3601 aagaatgtga gttgatccgt ggcgagcctc aatcttgtcc aggtgctcaa cggcctgacg
     3661 ttcgcggcat tgcttttcat cgtcgcgtcg ggtttcactc tggttttcgg cctgttgcgc
     3721 atcgtcaacc tggcccacgg ggcgctttat ctgttcggcg gatatatcgg ctacaccgtc
     3781 atcgcaacga cgggcaattt tgtgctcggc gtgctggcgg cgatgctggc catcgcttgt
     3841 atcggccttt tcgtcgatca gaccttgttg cgcttcgtgc gcgggttcga gctgcgcgag
     3901 gtcctattga cgctcggcgt cgctttcgtt ctcaacgatc tcgctttggt catttggggc
     3961 ggcgacacgt tctcggtgca agttccggag tttctccgcg gcgcagtgcg cttcagcggc
     4021 atcttctatc cgaaataccg cctgttcgtg ctggtccttg gcgttctcac cttcgtcggg
     4081 ctctggcttt tgatgaaccg aacgcggctc ggcgcactca tccgcgcggg tgtggatgat
     4141 gccgagatgg tcgaggccat gggcatcaat atccgccgcg tgtttctcgc gacctcgatg
     4201 ctcggcgctt cgctggccgg actcggtgga acgatcgggg gcgcgtttct gacgctctat
     4261 ccaacagcag actccgagat tctggtcttc tccctcgcga tcgtgatcat cggcggcagc
     4321 ggcagcctcg tcggcgcggc cgtcggcgcg ttgctcgtcg gcatgctcaa cacgatcggc
     4381 caggtcctgt ttccggagct cgcatacttc gtcattttcg gtccgatggc ggtgctcctt
     4441 gccttccgtc ccctgggctt gttcgggagg gcagcctgat ggcgtcggcg atcacttccg
     4501 ttcggcattc gccgctggcc ttcatcgtcg tgctggccgc cattttcggc gccctgccgc
     4561 ccctcgtctc gacgtacgag ctgagtctcg ccaacgaggt gctgatattc gcattgctcg
     4621 tcatgtcgat cgacgtgctc gccggctacg ccggccgtgt atcgctcggt cacggcgcga
     4681 ttttcgggat ctcgacgtac gtcgtcatct actggacgac gatcatgggc ggatcgccct
     4741 gggtcggttg ttttctcggt atactcgctg cgactttcgc ggccggcgtt ttcgctctgc
     4801 tggccgcccg ggtcggtggg gtttacttcc tgctgctgac ccttgccttg ggcatgatcg
     4861 tatggggcgt atgcctgcgg tggaccagcg tgaccggcgg tgagaacggc attcgcggct
     4921 cgggccgtcc cgacatcatc gccgatccga ccgtcttcta ctatgtgacc ctggccgtgg
     4981 ttcttgcttt gaccgtaatc atctggcgtt tcgttcactc gcccttcggc ctgacgctcc
     5041 gcggaatccg cgacagcgag agccggatgc ggagtctcgg ctacggcgca gcgacccaca
     5101 tctttctggc gttcacgatc acaggccttt tcgccggggt ggcgggcgcg ctttacgccc
     5161 tgttcaacga tttcgtcagt ccctcgaccg tgcaattggc acaatcggtc gagggcctcc
     5221 tcatggcgat catcggtggg ataggcacct tgttcggctc tttcatcgga gcggccgcca
     5281 tcattctgct ggagaatttc gtgagccagt acaccgagcg ttggcagatg atcttggggc
     5341 taacgttcat cggcgcgatg atctttgcgc ccgagggcat tctcggtgca ttgcgcaagc
     5401 tgtttgcgag gagcagtagc cgccaaacga agagctgata ttgggttctc atagacggag
     5461 gaaacgatgg atcgcaggac attcctaaag cactcaggct tggcgctggc cgcaaccgcg
     5521 acgagcgcat tcccaatgcc tcatatcgcg cgggcacaag ccgggaaggc gatccgcatc
     5581 ggcctgatgg caccgctgag cggtgtcgtg gctgcaggcg gcaaagagat cgtcgaaggc
     5641 ttcaacatgt attgggagga gaaaggcaat cagatcgccg gccgcccggt cgaggtcgtc
     5701 gtcgaggacg acgccggcaa cccggatacg gccctgcaga aagcccgccg cctcgtcgag
     5761 caggaaaacg tcgatttcct gttcggcaat ctgctcgcca acacgggact ggcggtcgcc
     5821 aattacgtcc gcggtaatgg cgttccctat ttcattccgg tcatcgccgc cgatgacctg
     5881 acgcagcgcc agcgcgtgaa gaacgtcatc cgcgttgccg gctacaccgc gagccagatg
     5941 ccgaggccgc tcgccgattg ggcgctgaag cagggctata agcgcatcgc cacgatctcg
     6001 caggactaca ccttcggcca cgagcagtgc gcgggctttt cccaggtgtt cacggaaggc
     6061 ggcggcacga tcgtcggcca gctctggaat ccgctgaaca ccgctgattt cagcccctac
     6121 atcgggcagc tcggcagcat gaatatcgat gccatcttca cgatggagct cggcgccgac
     6181 gcctcccgct tcatgaagca gtattccgat tttggactga agggccagat cccgctgctc
     6241 ggcgcccaga acttcaccga ccagtcggtg atccgcacct cgggtccgga gtgcgagggc
     6301 atcgtctcct ccgcacattt cgccgagggc tccgacaatc ctgaaacgca gaagtttgtg
     6361 aaggcctaca gcgagaagta tggcaagttg ccgtcgcttt acggcttctc gcactattcg
     6421 ggtgcgctat gggtcgggca ggtcatcgag gggctcaaag gcgacatttc gaaccgagac
     6481 aagtttctcg acgcggtgct gaacagcgag ctcaccaatt cgcctctcgg tcgccccgtc
     6541 cgcttcgacg actacggaaa ccccatctac gacgtctata tccgcaaggt cgtgaagaac
     6601 gccgaaggaa aatactggaa cgtgccgatc gagacgtatc cctccgtttc gcaattctgg
     6661 acctacgatc cgaaagagta tctggcgaag ccgccgttct cgcgcgacaa ccagggcatc
     6721 aagaaaacct gattcattcg tcagggcgta gccgcgtctg cggctgcgcc caccatacta
     6781 cctgaacaag agcttcgttc gtgtcggaaa ctgcgcttgt ccttgagaat atcgttgttg
     6841 cctttggcgc gctgaaggcg gtcgatgacg tgtcgatctc cgtcaggcgc ggcgagcgac
     6901 gcgcgatcat cggcccgaac ggcgcaggca agacgaccct cttcaatgcg atcaccgggg
     6961 ttttcccgcc gacgtcgggc cgcgtcgtat tcgaaggtca gaatgtgacg cgcatgccag
     7021 cgcagaagcg tgcgcggatg ggcatgagcc gcaccttcca gatcaccaac ctgtttccga
     7081 ccatgaccgt cgaggagaac atgcagctcg cccttcgtgg gaccagccgc agcaagttct
     7141 cgatgctcgg ttcgacccgg ctgaccagcg aggagcagga ccgggtggcc tccgcacttc
     7201 gcgtttctcg gctggaggaa cgcgtttccg tcgcggtgaa cgagctttcc tacggcgagc
     7261 agcgccagct cgaaatggcg atggcgttga tttccaaacc gcgcttgctt ttgttggacg
     7321 agccggctgc cgggctgtcc cccgccgagc gcctgatggt ggccgagatc atccgctcgc
     7381 tgccgcagga tttgaccatg gtcctgatcg agcacgacat ggagctggtg ctcaatctcg
     7441 tggactgggt gacctgcctc aataatggcc ggctgctcgc agaaggctcg ccggaggaca
     7501 tccgctccaa tcagcacgtg caggacgtct atctcgggaa ggcgcggtcc gatgcttgag
     7561 gttcgcaacc tgcacagctt ctatggcgac gcgcacgtgc tgcatggcgt ctcgctcgag
     7621 gtccggcccg gcgaggtcgt ggcgctcctc ggtcgcaacg ggatgggcaa gacgaccctg
     7681 atccgctcca tcatgcggct ggcgcagccg caagtgcggg aaggctcgct gttgtggaaa
     7741 ggggaagaac tcaggaattt ggccccgcat gaagtcgcct tgcggcggat cgcgctggtg
     7801 ccgcaggggc gcaggctgtt ttcatccctg accgtgacgg agcatctgac gatgctgaaa
     7861 tcggccagct ccaagcaggg ctggacggtc gaccgcgtct tcgaaatgtt tccgaggctg
     7921 gccgagcgca agaaccaccg cggcaaccag ctttcgggtg gcgagcgtca gatgctcgcg
     7981 atcggccgat cactgatgat cgatcccgag ctggtgctga tggacgagcc atcggagggg
     8041 cttgctccag tgctggttca acagctggag acgatcatcg ccgatctcgg caagactggc
     8101 ctcgccattc tgttggtcga gcagaacctc tacagcgctc tctcggtggc ctcccgtttc
     8161 tacattttgg aaacgggccg tgtggtgcat gaggccaccg cggatgaaat caagaaggat
     8221 caagcgccgc tgatgcgctt tctcggggtg cactgacagt gaagcgttgg gacctgttca
     8281 ttggcgggga ataccgcccg gcggagtcgg ggcgcacatt caccgtcgaa aatccaaaag
     8341 acggctcgat cattgcggag gtggccgaag gcgacgcgca agacatcgag gctgccgtcg
     8401 ccgcggccga gcacgccttt ccgcaatggt ccgccttgtc gggcgccgag cgtggcgaca
     8461 tcatgcatcg catcgccgag atcctgcggc ggcgcctgcc cgagcttgcg accattgaaa
     8521 tcgatcagat cggccgccct cgccgcgaga tgacggcgca attggcgcgc ctgccagagt
     8581 ggttcagcta cttcggcgcc gtagctcgca cgcacgagga caccgtcccc ccgttttccg
     8641 gcgattatct taattatacc caccgcgtgc cactcggagt ggtcgggcat atcacgccct
     8701 ggaaccatcc gctgctgatc ctcaccaaga aggtggcccc ctcgctggcc gcaggcaatg
     8761 cgatggtggt caagccatcc gagctggcac ccatcacacc gcttctgctc ggcgaaattc
     8821 tcaaagaagc gggcgtgcct gatggaactt acaatgtcgt gccaggcttc ggcgccgctg
     8881 caggcttggc tctttcctct cattcacgta ttcgcaagct cgatctcacc ggcggaaccg
     8941 agaccggcaa ggcggtggcc tccattgcgg gcggtaattt ggtgagcgtg tctgccgagc
     9001 tcggcggaaa ggctgccgtg atcgcttttc ccgactcaga cgtcggacgt gttgtcgccg
     9061 ctaccatgtt cgcggcgttc atcgccagcg ggcaaacctg cgtccagggc tcccgtctgc
     9121 ttgtccatcg ctcgattcac gacgaggtgg taagcgagct cgtccgccgc acgcgggaga
     9181 ttcggctggg cgaccctcag ctcgagacga cgcagatggg ccccctggtc tctagccggc
     9241 aacgcgagcg cgtggaggaa tacgtgcgga tcggcatcga ggagggtgcg catctcgctt
     9301 acggcggcgt gcgtcccgaa tgcccttccc tcgccgcggg ctattatttt ctgccgacca
     9361 tcttcaccaa tgtctcccct cagatgcgaa tagcgcagga ggagattttc ggaccggtcg
     9421 tctgcgtcat tccctttgag accgagcagg aagccattgc catcgcgaat ggcacggaat
     9481 tcggcctggc gaccagcatc tggacccggg acatcgcgcg ggctcatcgc gttgcccatt
     9541 ccctgcactg tggaataacc tggatcaacg atcatcaccg catcgatcct gcctcgccct
     9601 ggggcggttt caagatgagc ggtatcggac gcgagaacgg catcgttgct tacgaggcct
     9661 acacacaaat ccagaacatc atcgtcaatc tttcggacag tacttttgat tggtatgccg
     9721 aggatggtca acagaagcgt tacagctgaa ctttagtagc ttttggtttc ccgccccaac
     9781 tgctccggat gctcatcacc taagcttcag caaattggcc gcgttctctg aaaaaatcgc
     9841 attttgttcc gagggtgcta attgggcctg ccgctccacc acttgcacgg gccgctcgta
     9901 gcccatgtcg aagcaataat cgcttccgag aagaacccga tccgacccaa ctgttttgat
     9961 cagataggcc agtgcgtcgt ccgagtgtgc aatcgtgtcg taatagaatc gtcgcagata
    10021 cgtttccggc ccctgctgcg tgtgggcgag ctccggacgg acggtccacc catgctgcag
    10081 ccggccgatc agaaacggca gagcaccacc tgcgtggggc agcacaaact ccagcttcgg
    10141 aaagcggtcg agaatgccgc cgaaaatgag gtgtgctgcc gcgactgccg tatcgaatgg
    10201 attgccgaga agattgtaga ggaagtacgg tttaagtcga tcctgcattc caatgacttt
    10261 caacggatgc aggaaaatcg gcaaaccggc ggcctcaatc gcctcgtaga ccgggaagag
    10321 cgactgatcg gaaagatcca tgtcgccgat gcaggtgccc atgtagatgc cgcgaatccc
    10381 gggcgcttgg cgaattcgtt ccagctccgc caaggcttcg cccgcatgat gcaccggcag
    10441 catcgccaag ccgaataggc ggcttggata acgttcatgg gcgcgcacca gatcatcgtt
    10501 gaaagctgcc gacaggcgac gcgccaaaga cggctcagcc cagtacacca tcggctgcgt
    10561 caacgacagc gcctgcgccg acacgccctg ggcgtccatg tccgcaattc gcaaatccag
    10621 atcgatgaac cgtggcgcca gcggcccagc gtggagcggc cctgccttga cgaccgggcc
    10681 acgcggatcc cgctcgacgc aggcgccaca agagcaaccc gcatcttcga tcaattgcag
    10741 gtagcgctcg gaatagtagt gggcgtggat gtcgatgcgc ggcggaattc gggaggacgt
    10801 catttcgaac ctttgcagtg gcgcagccga tgcagactat atcgagcact ttggcagcaa
    10861 tgctaccgcc tcgccgcggc aatccgcaga tgcttttgga aattccggct gcgtgcagct
    10921 ttcgggcgtc agacgagccc acaatctgca tcggaatgct gcgctaacgg cggggatgat
    10981 ttatactggc gctgctgcaa gccctcctct gtggtgttca gtgcccgcgg atggcactca
    11041 ccgcctcccg aaatttttca ggagttccct ccccatgact gagcaacata tcgacccgaa
    11101 cctgatccgc agcaggctcc catacagccc gattacccaa cggccgccgt tgcggcttcc
    11161 caaaggcgaa cgcgtcgccg tctggaccat cgtcaacgtc gagaactggt cacctgacaa
    11221 cgcgatgccg cgaaccgtgc tgccgccacc catgggccaa ccgctgctac cggatatccc
    11281 gaactgggcc tggcacgaat atgggatgcg cgtcggattc tggcgttttc tggaggtgct
    11341 gggggcgcgc aatctcaagg ccagcttcgc cgtcaatgga tcggcctgtc gtgtctatga
    11401 agctgcctgc aaggccgccc atgaggccgg atgggacttc atcggccacg gtttcatcca
    11461 gcgcccgatg caccgcgtcg ccgatcagcg ggcggccatt gccgacacta tcgaggcgat
    11521 ccgctcgctg accggcaagg cgccgcgcgg ctgggaaagc cctgggttga ccgagacggt
    11581 cgagacgctc gatctgctgg cggaagccgg aatcgagttc gtctgcgact ggtgcctgga
    11641 cgatcagccg gtgagaatga acaccaaggc cggcccgatc gtctcaattc cgtacacggt
    11701 cgaagtcaac gatgtggtga tgacagcggt tcaaggacac cgctccgacg agatctacaa
    11761 gcgcggcgtc gaccagttcg accgcctcta tctcgatggg gcaagaacgc cccgtgtgat
    11821 ggcgatctcc atccacccat tcctgacggg cgtgccgcat cgcatcaaat atctcgagat
    11881 gctctacgac tacatcctgg ggcacgaggg ggtcgcgatg atgaccggct ctgaaatcct
    11941 cgactggtac gttgcgcaga cgagctagtg gcgaagctgt tcctgccagg caggccctgg
    12001 aaaacccgta tagcgcgggc gggccggtgg gagctgttgg gccgctcaac gaatggtttt
    12061 cgaagaattg ctctccggca gttcagaccg gaggcgcgcc tcctcctcgt atgagatgtc
    12121 ggcggtgatg taggccagga cctcccgtgc atgcgtattg ggcggatgca cgggatagaa
    12181 cacctgctcg atccgcccgt cctgcagaaa cagcgtcagg cgcttcaagt agcgggcgcc
    12241 gcccgtctcg aaaaccggca gatcgagagc cttctggaat gcctgcgccg catcgctcaa
    12301 cagctcgaac ggcagcgcga gccgagacgc gaactcctgc tgctcctcag tcggctggag
    12361 cgacaggccg aacacggccg tcggcatttc ggcaaatccg ggatacagat cgcgaaagcc
    12421 ttccgcctcc ggcgtcgagc catgcgctcc cggtattgtg tcccaatcgg gcgggttcgg
    12481 cacgcccggt cggccggtcc agggataaca gaacacgact gcccggccgg gaacgcgcgc
    12541 cagcgatacc atccgtccgc tcgtcgccgg tagcgggatg tccggcaggc caaggccgca
    12601 gacgaggtga tccgcgctgc cgtcatcgac cggcgcaggc cagggccacc tcatgcctgc
    12661 cccgccatct gataccgctg caaccaggcc agaacgtcgc ccgcattccg gtcaggtgga
    12721 aacacggggt agaagacctt ggcgatttcc ccgtccgcga tcaccatcgc gagccgcttc
    12781 agcagcgtca ttccagcaac ctccatggtc ggcaacttca gagcccgtgt gagcgcaagg
    12841 tcctcatccg agagtaccgg gaatggcaga tggagccgat cggccatctc gcgctggtat
    12901 gcggtgtcct gcgtcgacag cccgaagacg tgcgcagccc cggccgcgac gagatcctgg
    12961 taatggtcgc ggaacgcaca gctctggggt gtgcagccgc gcgcgccggg gatcatgtcc
    13021 cagttctcaa ccagcgagat ttcccccggc cgaccggtgc gcgggtaagc gtacaccact
    13081 gtcagtcccg tgaggcaaga gaggcaaaca gaactgccgt ccgttgcggg aagtgtcaca
    13141 tcgggtacgc gcatgccctc gagatgccgg gccgcaccat cgtccgtcgg tgcagggatc
    13201 gtcgaccaat cgacggtgtg caagtcgggg ttgcgggtag acatcgggcg agatcctcag
    13261 cagtcgcgga cccgatagtg gtagccgttc actttcctgt atccaagcgc agcgtagaac
    13321 cgttgcgccg gttcgttcgc agctaccgcc tgcagggctg caatcctcgc gccccgcgca
    13381 gagccccagg cttccaaggc cgccatcacc ttgcgcgcgc ccccagtccg tctggcctcg
    13441 gcgcgcgtgc cgacgcactc cgcgatcacg atgtgatcat gtaacacggc cagcgccgta
    13501 ctgacgacct gcccaccgga gacgtagctg aggaacgcgc gcggagccgg caccgcgtcg
    13561 agaatggctg gcgccgcggc atgccgattg ggcgcaatgt tggagaggta gacctccatc
    13621 cagcccgccg tcggctgggc cgttatctcg atgcccgcgg gccacgatgg ttgccccaaa
    13681 cgacgggcga gcgtcgtcac cggctcctcg aggcgataac cgcgcctttc aagttgcaga
    13741 tcgaggtcgg caggggcagc aagctcggtc ccgacctgaa accggctgac ggcgccacgg
    13801 cagaagtaga gctcttcggc ctcttcaatt gcggcaatgg tgtcagcccc gtcaaaggcg
    13861 agcgttgaga ctgagttcgc ccgctgcgag ccgcccccgg agtaccgcca cagccagccc
    13921 gcgatgtttt gcgtctcgga cgccggccag gcgtggacgg ccgccctttc gatcgcgagc
    13981 aggtcgtctc gagtcagggc gaggcaaacg tcggcagaag catccatgtg atcgccccct
    14041 ggcgtcgtga tcggggaaat tcgtcatact tgagggagga aaggcaagcg aaattctgga
    14101 cctgaacgaa gcggatttcg cgatccgggc accgattgtc ctgcccgatg cggaaatgcc
    14161 ctctcagccc ttctcccgca gcagccgcgc cttctggcgc tcccagtccc gcttggcttc
    14221 ggtctggcgc ttgtcgtgaa gcttcttgcc ttgggccagg gccagcttca gcttggctat
    14281 accgcgctcg ttgaaataga gctccagcgg caccagggtc atgccctgcc ggcttatcgc
    14341 gttggcgatc ttgtgcaact cacggcgatg catgagcagc cgccgcggcc gcctcggctc
    14401 gtggttgaag aactcgcctg cctgcttgta ttcggggata taggcattga tgagggccaa
    14461 gcccccatct tccatggcgg catagctctc cgagatgttg gcctgtccct gccgcagtga
    14521 tttcacctcc gtgccggtga gcgcgatgcc cgcctcgtac gtatcggtga tgaaatactc
    14581 gtggcgggcc ttccggttct cggcaacgag ccggcggccg ccattcgtct tggccgccat
    14641 gatttcggaa actcccgcct tcggacctac tcggccgcct tcgcctgcaa cagcccggcg
    14701 ccaaccatcg ccgattcgac ggcgcgcttg cttgcctccg agatcggtgc gagcggcagg
    14761 cgcgtctcgg cgctgcacag cccaagcaga gaggcggcgt acttcacagg ccccgggctc
    14821 gtttcgatga agagcgcatc gtgcagcggc atcagccggt cctgaatgct gagcgccgtg
    14881 gcgaagtcgc cgcggaggca agcgttctgg aactcggcgc aaagcgcagg cgccacgttc
    14941 gatgtcaccg aaatgcagcc ctggccgcca tgggcgttga accccagcgc ggtcgcatcc
    15001 tcgccggaca acattacgaa cccaggcccg caggcgagcc gctgaagcga ggcgcgggaa
    15061 agattggccg tcgcgtcctt cacgccaatg acgttcttca gctgagcgca gcgcgccatc
    15121 gtcgccaccg acatgtccgc cacggtgcgg ctcggcacgt tgtagaggac gatcgggatg
    15181 tcgaccgcat cgttgatggc cttgacgtgg agataaaggc cctcctgcgt cggcttgttg
    15241 taatagggca tgacgatgag cacggcgtcg gcgccgacat ccttggcgtg cctggtgaaa
    15301 tcgatcgcct cggccgtgga gttggatcct gcgccggcga tcaccggcac gcgcttgcga
    15361 gccgtctcga cgcagacctc gaccgcatgt ttatgctcct cgtgagacag cgtcgggctc
    15421 tcgcccgtcg ttccgaccgg aacgaggccg tgggtgccct gagcaatctg ccactcgatg
    15481 agccgctcgt aagccgacgt atcgaacgca ccgttcttga acggcgagat cagggccgtg
    15541 aaggatccct tgaacatgtg tgctgctcct cgagcagagg ccgcccgcac cggtcggagg
    15601 taacattgcc ggaagcaggc cgggcattct tccttgaacg cggcacgggt gcttggtagc
    15661 ctaacggcgg tatcaggccc ccacaccgac cgtaccttga taaggtcagg aacattatgc
    15721 ggttcaaggg gtcttcgcaa ggcgaagctc ctctgaatgg tgttgaggga gcttttagca
    15781 gccggtcgcc aaaatggcac cggaatagac agactgccga tcaccagctt ctggacctga
    15841 gacgagacgc tcgtgccgat tcgcgttccc gccctccaag gtttgctcac cgcatcagcg
    15901 attgccgcga tggcggcctt ctggccggcc acagcggcca atccgaagct tgtgcccgca
    15961 ccgtccgctg cccccgtggt caaggcgaac attggccggt ttccggccaa gccgttgctt
    16021 acgcaggacc acgctgcgcg cgaggccgat cacatcgcgc gcttcgatgc ggcgatcgca
    16081 gcggcgcgcg ggcatgaact gagcgaggat gatgccaagc gcatcaatga tgcgatcggt
    16141 gcctattacg ccagggacat cgaaaaggcg cgagcgatcc agaacgacat caccgatccc
    16201 atcggccgca agctcgtcga gtggtatcgc ctgcgcgctg gcctcgccga gccgttcgag
    16261 atccaggcct tcctcgacgc caatccagcc tggcccgatc gcgatcgcct tagagccaac
    16321 ttcgagaagc tcctgttcag caaaggcggc aatgcgcaag tcatcaaaga ggcgttcgcc
    16381 aagtccgagc cgacgacggg cctcggcttc gcggcgctcg cctctgccta tctcgtcttg
    16441 ggcgacaagg agcgcgccaa ggcgctcgcc gcctacgcct ggcgagaaga gtccatcccc
    16501 gaagagttcg aggctggttt tctcgagcgc ttcggcgggc tgctcacgga ggccgatcac
    16561 aagtggcgct tcgatcgtct gctgatcagc gatccacgct ggtcgagcca gcgcaaggcc
    16621 cgggctgcag tcgccaagcg ggtcattcca ctgctcggca aagcggagca gaagaaggcc
    16681 gaggcgcgcc tggccgcctt cctccgccgg aaatcagcgg caaagcagct gtccgcactc
    16741 ccggccgatt cttctgcgga ctggggattg gcgttccagc gcgccgacct cttcctgagc
    16801 caaggtaagg acgagcaggc ctggaagatt ttgctgtcgg cgcccaccga tccgaaagaa
    16861 atcgtcaatc ccgatggctg gtgggagcta cggcgcgagg cggcatacga ggccctccat
    16921 gccggcaagc cgaaggtcgc gttcgagctt gtccgcgatg ccggcccgct cagcgtcaac
    16981 ccgcgcaacg agcagacgtt cttcgcgggc tggctcgcgc ttcgccacct caaccagccg
    17041 aaaaccgcct tgcccttctt cgaggccaac aaagacggat ccgatggtcc gctcagccgg
    17101 tcgcgggcgc tctactggct gggccgcacc cacaaggcgc tcggcgaaaa caagaaggcc
    17161 cgggaggagt tcgcggcggc agccgcctac atcgacacgt tccatggcca gctcgcccgc
    17221 gaggagcttg gagacgaccg cccggaactc gttatcactg cgcctgagga gccgaccgac
    17281 gagcagatcg cgcgattcaa ccggctcgat gccgtccggg ccgcagtgat cgccgccaag
    17341 gccggcatca atcgcactct gacgcgcgcc ttcctttcgg agctgcagca ctacttcgga
    17401 gccgaagccg aagtcgcgat ggtggcccat ctggcacaag cgctcggcga caatcagatg
    17461 gccgttcgga tcggcaaggc ggccatcgcg cgcggcatga acctggtgca ctactcctac
    17521 ccgatccatg ccttccccgc ctacgagccg ctccgcacgc cgcctgagcc cgcgctgctc
    17581 ctcgccctcg cgcgccagga aagcgagttc aacagctcca tcatgtccgg tgcgggcgcg
    17641 cgcgggatct tgcaggtcat gcccggcacg gcacgccaca tctgccgcag ctacaagatc
    17701 aagtacaatg tccgcaagct gatggcggat ccttcctata acgccatgct ggccagcgcc
    17761 tacgtcggcg accggatgga cgatgtggac ggcagctaca tcctgacact cacgagctat
    17821 aacgccggcc caggacgcac gcggcagtgg atccgcgagt tcggcgaccc gcgcgatccc
    17881 ggcgtcgacc cgatcgactg gatctatcag atcccgttcg aggagacgcg ggactatgta
    17941 cagaaggtgc tctcgaacgt gcagatctat cgggcgcgcc ttggcaagac gaacggctcg
    18001 atcgtcgccg atctcacccg cgcgcgtcgc aagccgcagg aagcgccata atcgagcacc
    18061 agaaccagat cagcggacaa tcaatggcaa acgagcccga gccctggaaa gacatcttct
    18121 tttcggcgcg cgacggcctg aggctgtatg cgcggcatta tcctgccccg ggctccaggt
    18181 tgaggcccgt gctctgtctc gcggggctga cgcggaattc gcgcgacttt cacgcccttg
    18241 ccgtcgccct gtcgaccggc gaagcggccc gggacgtgta tacgctcgac taccgcggcc
    18301 ggggacgctc cgcgtatgat accgactggc gcaactattc cgtcgccgtc gagatgctgg
    18361 atgtgcagga cctcgtgacc ctggctggcc tcgaaaaacc ggccatcatc ggcacctcgc
    18421 gcggcggact gataacgatg gcgctcgccg ccgcgcagcc gggtgcgatc ggcgccgtcg
    18481 tcctcaatga catcggaccc gtgatcgagc gcgatggcct gactcggatt gccgggtatg
    18541 tcggcaacac gcccctgccg acgagctggg aggatgcggc ccggttggtg gctgagctga
    18601 accgccgggc cttcgtggcc gtgccggaag aggactgggc ggagatcgcg cggcaatggt
    18661 tcaacgacga caagggctac cccgcacccg gctacgaccc ggcgctcgca aaatcatttt
    18721 cctcagccga tggcccgatc ccggatctgt ggccgcagtt cgctggcttg agccgcgtgc
    18781 cgctgctcgt cattcgtggc gagaactccg acatcctgag cgagacaacg gtgcagcaga
    18841 tgcgcacacg ccacccgcgt tgcgcgacgc tgaccgttcc gggtcagggc cacgcgccgc
    18901 tgctgaggga cgcgagcacg atcgcggcga ttgcggcctt tctccgagag gccgacgcgg
    18961 ttgtgctgag ctctgcgcaa gcggcctagc cgagccacct caaaccgggc gggctgagct
    19021 gagcccgccc ggaacgtgcg ctcagggtga cacggtcgcc gttaccggct gcgccggcgt
    19081 tccatggatc cgcggatcga cggggcccag ctcaccgtca tcgtggcctt cagagcccgg
    19141 catgccgata taatcgatct gcccgcgccc gtccggactg gggcccgagg cccagctacc
    19201 gtcctggctt tccgtgcctt cggagaagtt gaggaactgg taggaaacgt gccggaactc
    19261 gttctcctgc gggaagctcg acgggcacgg cgtcgactcc agcccgtcct cctccagctc
    19321 acgtatggct gcgagccatt ggttctggtg catggtgtcg cgcgcgatga gaaacgacag
    19381 catatcgcgc acgccggggt cggacgtcag ctcgtaaagc cggacgactt gaaggcgccc
    19441 ctggctctca gcggtaacgt tgtaccgaaa atcggcgagc atgttgccgc tcgatatcgt
    19501 gtagcgcgcg ttccagggat agccgacgct gtcggtcggg gttgcgcctg cgcccgagac
    19561 gatcacgtgc tgggggttca tggcggccat cagcacgtct tccacgcgag cgccgcccag
    19621 caccgcgccg accgccgagt tcttggccgc ttcctcgcgc atttcgatcg gagaagactc
    19681 gagcagccgg gcgatcatcg tcgcaagcat ttcgacgtgg ccgatctcct cggtgccgat
    19741 atcgaggatc atgtccctgt acttggcggg accgcgacaa ttccagccct ggaaaagata
    19801 gctcatcatg acgcttatct cgccccactg gccgccgagc acttcctgca gcttgcgggc
    19861 atatgccggg tcgggccgct cgggccgagc gttgtactgc aaccggttgg ttcgtgagaa
    19921 catcgacacg ctcctgtgtt cgggtttcag ccatcaacgg gagcaaatcg gttgtgttcc
    19981 agagccaact catggagcgg ggccaagctt ggcctcagtt gcgacatcga atcagctaat
    20041 ttgaggcgag cgacgacgtc cacgcgagcg tggcccattg gcggcgccgc gcgtaaacgc
    20101 ggcgcaagcc tcgtcgaggg acgtgaacag gatcgaggtt ggccatgtcc gataagacgg
    20161 ttcttgccgt tccggggagg aagctgtcgg ttcctcgcgt tttcaagaga acgctgcaga
    20221 tctcgatggc ggcccttctg acctccgcag cggctctcac cttcatcgtt gccaacagca
    20281 tgctgctcgg ccgtggcggg ccgcttcaag cgatctcggc atggctggcc ttcatcaagc
    20341 ggccggacat ccaggcgacg atcattctca cggctgtcgt caccgtgctt ttcgtctact
    20401 ggcagcgtga ccgcgagcgg aggtaaaccg ctctgcgagt agcccgttcg aaacgtagca
    20461 gaaatcggcc ttggttatct gcacgtccgc ggcgctgccg accatggttg catccatgat
    20521 gcggtaaagc gttaatccgg ctcccttcat caggctgtcc acgtcaggca gcgggttgcg
    20581 ttcacgctcg aacaggtagc ccatgttcac ctcgacgaac atgcagccgg tccgctgcat
    20641 cgtcctcgtc gccccctcca gaacctcgag ctccgcccct tcgacatcga tcttgaccac
    20701 caatcgctgg ccagggtgat ccgccagcat attgtcgagc gtatccatct ctgcccgccg
    20761 gacggtgcca tcgtgcatcg tgcggcgata gaggctcgac tggtgcggct tgcgcgcgtt
    20821 gaagtggaga tcgacaactc cggtgcgggc gccggcggct ttcccaatga tggttacggg
    20881 cctcgtaagg ggcgcaatct gttccggaaa ctcaaggttc ctttcgggaa caacgccgcg
    20941 tttggcatct tgtagtacct ttgagctgcg tcaccggtgc ccgcacccaa tgctgaaact
    21001 tgtaacctcc gctcactgaa attttcttat tcgagcgact tcgaacgcag gccatccact
    21061 cctccaactc ggtgtcattt ggtcaaatac cggctataca tttaatcgtc tgctccgatt
    21121 ttaattgcga gcagcgaacc gggtgaacct cgcttaccga actcttcagc agttggccca
    21181 tcttgaaggt ccgcgggtga gagattggag cgttgcgtcc gatgctcgcc ccctcaatca
    21241 gcaccagtca cctacgcacc ttctgctcag aacgccattt cgctccatta tactgcaccg
    21301 aatggtgtca gggttagacg cagcgcatac agggttgcaa cggaattaga tccgtgagta
    21361 cccaccaact ttgaacgcca ctattccttg acattaaagt ggttctgtaa tactctgcac
    21421 gtaatcgatt acgtcgtgca gaacaaggcg gccaacgtgc agggaggtct aacgctcggc
    21481 gccgatgttt gggcgcaaga aacactaaat tgcctcatgg gggcttaggc tgggatggag
    21541 ctgacaacct caccggctgt agcgatagag cggcgtggcg caatcgctat catcactatc
    21601 aataacccgg agcgcatgaa cgcattgagt gctagcgtcc gtgatgcgtt gcgccttgca
    21661 ttcgagggct ttgaggatga caggtccgtc agggctgtcg ttctccgagg aagtggtaat
    21721 cgcgcttttg cttcaggcgg cgatatttca gagtttccgc gaatggactc ggctgcggcg
    21781 cggtcaaact tcgagaaact tgaggccctc tttgcggcta ttgagcggct ccacttgccc
    21841 gttatcgcca tgattcatgg ttacgctctt gggggcggct gcgagctggc tctggcgtgt
    21901 gatttgcgta tcgcagcaag cgactcgaaa ttcgggattc cgattggcag attgggtcac
    21961 acgctcgatc tcgccagtgc ccggcgcctg gtgcgcttga tcggcgcgcc aaaggtcaag
    22021 atgctactct ggacggacga gattatagat gcgggagagg cccgtcgagt cggtttgatc
    22081 gacgaggtgg tttctcctga cgagctagag tcatacacgc tatcgctggc atccaaaata
    22141 gcggaaaagg cgccgctttc tatcgcgggt acgaaatctg tgctcgcaca cgtcaccaaa
    22201 catcctgtgg cgcggccggg cgaagatatc gcccaatttg cggcgccact ttttgaaacc
    22261 gccgatttca aggaaggcgt gcgcgctttc cttgagaagc gcaaaccaac ctttgtcggc
    22321 caatgacaca ctctccgctc cagggtattc gggttataga tttcacgcac atgcgggccg
    22381 ggccgcagtg cacgatgatg ctcggcgaca tgggggccga gatcatcaag atagaatcgc
    22441 cgcggggcga cggaacgcgc ctctatggac gcgacatcaa tggtgaggcg ctcgatttct
    22501 tatccgtgaa ccgcaataaa aagagcgtcg ttctggatct aaagagcccg gaaggacgcc
    22561 aggcggcgat ggatctgatt agtacggctg atgtcctggt cgaaaatttc cgaatcgggg
    22621 tcatggaacg cctcggtttc ggccgggagc ggttagctga actgtttccg cgcctgatct
    22681 attgcagtat aacggcctat gggcaaaccg gcccatacgc tgggcttccc ggatatgacc
    22741 agattgctca aggaatgtcc gggctcatga gtttgaccgg aacaaaagaa tctggaccgc
    22801 tgcgcgtcgg attgcctatc ggggatttgt tggcgggtgc gtttgcggcc tacggaattg
    22861 tgctggcact tttcgagcga cagcactctg ggcgagggca gttcgtccat acctcgttgc
    22921 tgcgagcact cgtgagtttg ctgagctttc aagcagcccg atatctgatg acgggagagg
    22981 tgccggatgt tgtgggtaat catcatccga tcgtagttcc gaacggcaca ttcgcgacca
    23041 gtgatggttt gataaatatc gggtgttcga cgcaggagca atgggaaagt ctt
//
LOCUS       DASVDU010000129        17540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1185171, whole genome shotgun sequence.
ACCESSION   DASVDU010000129 DASVDU010000000
VERSION     DASVDU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17540
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185171"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VF226_08200"
     CDS             complement(<1..1309)
                     /locus_tag="VF226_08200"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7074006.1"
                     /translation="MRPSNTTIAAPHANAVGVGATLQDGLDVLDPELSRLVVQERRRQ
                     NETINLIASESYCPRATLEAESSILVSKNATGYPGRRAVAGCEIMDEIERLAQARALS
                     LFGGEHANIQALSSTIANIAVLRGLLEPGARVLALDEAAGGHHSHGASYHLSGRDYHV
                     TLFGLDEVSGTIDLDALRRTALELRPAMIIAGSTACPRAIDFAGLAAVAREAGALLVA
                     DIAHVAGLVAAGLHANPTACADVVTTSTHKTLCGPRTGGLILSRAAHADRIDHALFPG
                     LQGAPGAHIIAARAALFAWAARPGFRALMQAVLDNARALAEGLREAGCQLYLGGTDTH
                     MIVVDLRAEPGSAAAIERRLAGHGVLTNRVPLPRRPGDRGEGGLRIGTTAMTLRGMGP
                     DDFAGLARLMGPVLKTGTEAAAIDANLSHRVLEMARAFPVPPSF"
     gene            complement(1319..1966)
                     /locus_tag="VF226_08205"
     CDS             complement(1319..1966)
                     /locus_tag="VF226_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7074007.1"
                     /translation="MESVVQSLPYLIEAATRTVMLSAVVIVISTAVGLVGGVVSVMFG
                     TIAQAVVLALVYVLRGVPILVQLFLVYFGLPFFGIRVSPYVVAVIAISLHMGALMTEV
                     FRGSLLALPRSQAEGGLALGMTPLMLMREVLLPQALRVALPPYVSLIPVTVKATALAS
                     VISIWELTLASKEIANRTLDTFEVFGIAFVMYFVICYPFTWLGRRFERKRTAYVH"
     gene            complement(1977..2642)
                     /locus_tag="VF226_08210"
     CDS             complement(1977..2642)
                     /locus_tag="VF226_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7074008.1"
                     /translation="MNAHMRFDIDYVIRHLPDFGPAILTALELLLLALAIAFAWGILL
                     AIGRLSKGPLRGAAAAYIEFFRNTPLLIQLYFAFFALPSLGIRLDAFETGVAALAAQH
                     SAFFAEIFRGTIQSVASGQREAGYALGMVPGQLMRYVVLPQALRNAVPAMGNQVILLL
                     QDTALVSTIGVYEITLQGRTLAERSAASFEMFVAVGLIYLVLSTIFSLLLRVVEQGCR
                     IVK"
     gene            complement(2639..3457)
                     /locus_tag="VF226_08215"
     CDS             complement(2639..3457)
                     /locus_tag="VF226_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7074009.1"
                     /translation="MIRIRSLRKRFGPLEVLRGVDLDVEKGEVVVLIGSSGSGKTTFL
                     RCLNFLAEYEAGQIHIGDKLMWYRDAECRKKRPIHEIARDRTRIGMVFQAFNLFPHRT
                     VLENIILGPTRIKAEPLSRADAESLAMELLRRVGLEDKARVYPATLSGGQQQRVAIAR
                     ALAMEPEVMLFDEVTSALDPELVGEVLNVMRRLAQDGMTMIVVTHEIPFALDVADRVI
                     FMDEGRILEQGPPAELLFNPQAPRLREFLRRFEEMRLITERMSGNGGQSAEALP"
     gene            complement(3524..4369)
                     /locus_tag="VF226_08220"
     CDS             complement(3524..4369)
                     /locus_tag="VF226_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7074010.1"
                     /translation="MKPRIRSMIWAIGAATAMAFAAGQAMAQQTSKLYEVLDRGHVIV
                     GVTSEVPPFGSVGENGELVGFDIDIARLIAKALFEDPDKIEFKKLAFPARWAAVNNGE
                     VDFGIMVTTIWPDRLARVNFTRGYIRSGLSLLTRTDIEADEIQDFNNSDYTVAILDTP
                     SEHKLMAEKLPEAKTLVLPSEADMLTAVRTNRADALLIDQPVAAWRVRNIPDIKNLGI
                     VGSSTQNAIFMRQNDFQWWYYLDALVQEMRCGSLYEEYKAIYNKWFNVDPPSPDRCIA
                     FAASK"
     gene            complement(4405..5397)
                     /locus_tag="VF226_08225"
     CDS             complement(4405..5397)
                     /locus_tag="VF226_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7074011.1"
                     /translation="MNAASRIVLLSTGGTIGERDNPATGCLHASGEDLLASAGVAGEV
                     HVENLFDLPSTFIGLPEMLRIAEAVAAAFRNGADAVVVTHGTDTLEETAFFVDLQHDD
                     ERPVVLTAAMLPDGVAGTDGVRNLRHAFLAAASPQLCGLGAVVVMEAEIHGARDVTKF
                     HSTSLGAFKSPEFGPLGSIDEDRIFVARRPARRTILPTTRRVGYVEAVKCYAGMSDCF
                     VRAAIAAAPDGLVIETLGSGQVPPVLMPAVREAMAAGIGIVATPRSPAGRLIRDHYEQ
                     PQRVTGDERDLRDAGVIFANLQGPKARIQLALAKGAGIAGEHLRRLFEEAEPDR"
     gene            complement(5394..6059)
                     /locus_tag="VF226_08230"
     CDS             complement(5394..6059)
                     /locus_tag="VF226_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7074012.1"
                     /translation="MAERLLAQMAGGTLLSERKLPPERILAETFKVSRGVVREALNAL
                     QMAGIVERHVGDGTYLAESIDPLQLKAVSLRQTLDASANVIEAIEAREALDLAVTNLA
                     IENAKADDLEVMDGILGRMRGAIDGGDVHRYLPLTLDLHAAIARAGGNSVLAGVVCYL
                     IDLIRPNLWIIEQNYSPSIADESFGIHKAMVDAIRERSRRKAQAAVRKHYRKYPSLQK
                     TSS"
     gene            complement(6736..7173)
                     /gene="gloA"
                     /locus_tag="VF226_08235"
     CDS             complement(6736..7173)
                     /gene="gloA"
                     /locus_tag="VF226_08235"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEX7074013.1"
                     /translation="MTTDTQAGKRADDITQYRLSHTMLRTRDLKQSLDFYTGLLGMKV
                     LRQTEYPDGKFTNTFIGYGAEVDHTTIELTYNWDRSEPYPTGEAFGHIAIEVPDVYAT
                     CERLARNGVKIVRPAGPMKHGSRVIAFVEDPDGYKVELNDAIW"
     gene            complement(7163..8416)
                     /locus_tag="VF226_08240"
     CDS             complement(7163..8416)
                     /locus_tag="VF226_08240"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7074014.1"
                     /translation="MLSTADCSVADLGAFVPGTEVRLAGAAEGPLKGLLFAAKDNFDV
                     AGFVTGCGNPDWARTHPPAAAHADAVAKLLGAGADLAGKTITDELAYSLAGQNPHYGT
                     PVNPAAPGRIPGGSSSGSASAVAGGIVDFALGSDTGGSVRIPASYCGLFGMRPTHGRI
                     SVKGLVPASPFCDTVGWFARTAFVLRQVGEVLYGVTAKDDIAVSELLLAEDAFGLLDP
                     AVRAVLTPHILTFEARFGRARAIDVAQPAGSFAKLIPSCRILQGREIWRQHGAWIETA
                     RPRLGPEAQGRFAWARGLTAAAEAEAGEVQDDLADSIHKLVSGNRLLLLPTAPGIAPL
                     TTATAEELSNYRDRVLGLTAISSLSGVPQITLPLVTLEGCPLGLSIIGAAGRDLDLLR
                     LAEMMTGGGFSNSASTANRSNDNDN"
     gene            complement(8423..9616)
                     /locus_tag="VF226_08245"
     CDS             complement(8423..9616)
                     /locus_tag="VF226_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074015.1"
                     /translation="MAESLSSRRSTEKLSLGRFPQTNNRSATKPEVSAVHRVGASIAL
                     SNVVKRYGTFTAVSRLSLAIEAGEFVSFLGPSGSGKTTSLLMVAGFIIPTEGAILLGN
                     QDITRLPPHRRNIGMVYQNYALFPHMTVFKNVAFPLRMRRLGKEAIRARVRQALELVQ
                     LQDMADRYPLQLSGGQQQRVALARALVFEPPLLLMDEPLGALDKKLREELQGEIKRIQ
                     RKIRSTVIYVTHDQDEALSMSDRVVVMKDGQIEQVGSPKELYETPRTRFVAGFLGSAN
                     FLDATLVSTGDISVARTEHGTVVKTSGRTQACVGSRVTLAVRPERVAVTLTQVPDNTG
                     YQGRVVDANYFGTTQRYEIRLDNGEILLANRTNIGDPLIEPSSTVFASWRPEDVWIIP
                     AAGNG"
     gene            complement(9671..10495)
                     /locus_tag="VF226_08250"
     CDS             complement(9671..10495)
                     /locus_tag="VF226_08250"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074016.1"
                     /translation="MKWSRDIFTLLRWGTWFYVACVLLFLITPLLVVIPAAFSDSTLL
                     QFPPEHWSTRWFEKYLSSPDWMVATWNSFKVAVAVSILATSLGLTSAIVLVRFRFIGR
                     NALRAVILAPLIVPVIVTAVAIYYFILQIGWAGNLWALILSHTVVTYPYATVVISSAL
                     AEFDQRLEDAAVGLGASRVRAFFEVSLPIIRPAVIVSFLFAFLLSLDEVVITIFVSGP
                     ETMTLSRRLWDGVRFELEPTIAAVSTCLIVTSSVIVLAAELLRHFLMGRVKVSARR"
     gene            complement(10492..11334)
                     /locus_tag="VF226_08255"
     CDS             complement(10492..11334)
                     /locus_tag="VF226_08255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074017.1"
                     /translation="MTDFLLARLGFVTLITPGAVFLIICFIIPVALLLQQSFFDPDFT
                     FDFYRKAFSSPVYLSVFKVSFEIALVSTALSLLASYPLAFALIIAPTRLRALMLGLIL
                     LPFWTNILIRCYAWMLVLQTKGMVNSVLTSSGILSAPAELVFNTTGVLIGMTHYLIPI
                     NTLMLYSSMKGIDLQLLNAAKGLGADPLRAFVNVFFPLSLPALKASAVLVFVIALGFF
                     VTPALLGGRGDITVAMLISSYFSELLNWGFGSALATLLLIFTAALLILYYSFQRDAQD
                     RRAK"
     gene            complement(11433..12506)
                     /locus_tag="VF226_08260"
     CDS             complement(11433..12506)
                     /locus_tag="VF226_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074018.1"
                     /translation="MKNSVKMTRRAFTSAVGAAALGIPLGLTSMKSAKANDDTVVFAG
                     WGGNWQEAEREYFFKDFERETGIRVVDVPDLDLAKVKTMVDSGNVSWDVVQALDMWVA
                     DKDKAASLWEEIDYSEIQSEGVPKDLLRPYGAGVATFAMILAYNTKAFEGKQAPASWA
                     DYWDFEKFVGKRGMIDAPRYALEIALMAKGVPPKDLYPLDVEGALEHWDKIKNDVLWW
                     DKFPQSINLLASGELAVTMTSHGRVMSLLEEEPDTPIKIVWSQGILTTDFLSIPKGSK
                     RKKNAQKLISWMLDPKRQADYAKATGVGPSNFKALDSLDDATKKTLPSYYYQTGQIIE
                     FNADWWSTREQELVERWNFWQLI"
     gene            complement(12639..13274)
                     /locus_tag="VF226_08265"
     CDS             complement(12639..13274)
                     /locus_tag="VF226_08265"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX7074019.1"
                     /translation="MPIVPIAEKARDSMAVHGSAGAPGEEISYVDDGLYDSLHAFLRR
                     ARTRSEGPNLDDQELRNVEKFIYFEAALLDDRRYRDWLELFSDAFVYWLPSTRDPQDP
                     RHEVAINFDDRRRLLDRITLMETGVQVAQLPASRTARAVTNVMAWRDDDGFVGVRSNI
                     LISEYRLGRITTYAGRQDHRLEFRDGRFRTQFKIISLINSEEPQGNTTFIL"
     gene            complement(13360..14688)
                     /locus_tag="VF226_08270"
     CDS             complement(13360..14688)
                     /locus_tag="VF226_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7074020.1"
                     /translation="MQVPLNRPESSPKHGKYDHLVRPDGMIHRLLFTDPEIFREEMNK
                     IFGGTWVFLLHEAEIPNPHDFKVVTVGLRSTVVTRAPDGKITAFLNRCTHRGSPVCLQ
                     EQGNAPRFTCPYHAWTFSCTGEMLSASFPDGYGPSFDRAAHNLGRFPKVESYRGFVFG
                     CLNPDEVPLIDWMGPAREILDWSIDKDGLACERPDEVRVIKGTQMMYRGNWKHQNDNN
                     TDGYHTPFLHKATNIMNQRRHGSGKWLSHVKDNTSMICQYLGNGHKLGDHRAELRSSW
                     EQARPVPGRESEAATLRKAVGEEAAKDYLELTGRAGINLILYPNLFIMGNGAFAVFEP
                     VSVNETKVRYYTILPAHVPEQVNRLRVRSEEDFNNVGSRDDSDIMERVQHALETIPEM
                     EWLDLTRGMNRQTVSPNGVITSNKTDDTAIRGAYSHWLTLMNREPRTAVR"
     gene            complement(14843..16057)
                     /locus_tag="VF226_08275"
     CDS             complement(14843..16057)
                     /locus_tag="VF226_08275"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7074021.1"
                     /translation="MQITKLTSEVGWTFKEALPFPKAVRVGSRTIVGGVLPLNERGEV
                     VEPGDIEAQTHAVFQNMRRTLAGMGCRMEDLVRINTYYVYDGAEDQATRYWEKMTRVR
                     LQYFPDPGPVGTAVRVRGIPLEGALIQIEAEAVAPEKGRERKRIMPDESWDWSIPIPL
                     SQGWRIDDHVWVGGQIAADRAGRAVALGDIDGQTRNVMMHIANVLRDSGGSFADLIHL
                     KICYMHRGDSAEAKRGLETIMRVVDEVCPTAVHTPTTAFGVNLLYEGLMLEIDATAVI
                     GGRSSIAEGATENANWPARKQAAKRGNLVYVSGQSPARTHGLRQDALDALGQVLNALA
                     GLQVSPERLAKLTVFLSPGLDGDDVDRILPELGAALTARLGPNAPAVTVARVTGLPGN
                     DVGLQMDAVAIL"
     gene            complement(16118..17341)
                     /locus_tag="VF226_08280"
     CDS             complement(16118..17341)
                     /locus_tag="VF226_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7074022.1"
                     /translation="MLISATFIKKNRVMMERDEAVKLAQQLIRIPTYNPPGSEQACAA
                     YLGALLAEAGFQVEYHEFSPGRTSVIARLAGYRSSGQGKPICFTGHLDTVPIGSAAWN
                     DDPCGGQIKGGRLYGRGSSDMKAGIAAFVVAAIAQLDMLRNGPGIVLVLTASEETGCQ
                     GAFHLVKLGNVLGEAGAVVVAEPTANYPLIGHKGALWLKASATGVGAHGSMPEKGINA
                     IYRGARLASKMEAFGFNIAGHATLGSPTINVGTFRGGSSVNIVPDRAEVEIDVRTVPG
                     MSHGRVRDELLTYLAPDIAEIETTADLQHIWTDASDAWVQKVYKIVGRHLGVSPEPRG
                     ASFFTDACALKHAYPDAPVVILGPGEPHMAHQTNEYCRVDRIEQSVAIYTDILADWCG
                     PEGSRTLTSLESVDG"
BASE COUNT         3635 a         5456 c         5094 g         3355 t
ORIGIN      
        1 tgaagctcgg cggcaccgga aaggcgcgcg ccatttccaa cacgcgatgc gaaaggttcg
       61 cgtcgatggc cgcggcctcc gtacctgtct tcagaacagg gcccatgagt cgggcgagcc
      121 cggcgaaatc gtccggcccc attccgcgca gcgtcatcgc cgtcgttccg atccgcagac
      181 caccttcgcc gcgatcaccc ggccgccgcg gcagcggcac ccggttcgtg agcacaccgt
      241 gaccggcgag cctccgttcg atcgcggctg cggagccggg ctccgcacgg agatcgacca
      301 cgatcatgtg tgtgtcggta ccgccgaggt agagttggca ccccgcctct cgcaggccct
      361 ccgccagggc gcgcgcgtta tcgagaacgg cctgcatcag cgcgcgaaag ccggggcggg
      421 ctgcccaggc gaacagagcg gcgcgggcgg caatgatgtg cgcgcccggg gcgccttgca
      481 ggccgggaaa gagggcatga tcgattcggt cggcgtgagc cgcacggctc aggatcaagc
      541 cgccggtgcg cggcccacac agcgtcttat gtgtcgaggt ggtcaccacg tcggcacagg
      601 ccgtcgggtt ggcgtgtaga ccagcggcga cgagacccgc cacatgcgcg atatccgcga
      661 ccagcagagc gcccgcctcc cgtgcgaccg cggcgagccc ggcaaaatct atggcgcgcg
      721 gacaggcggt cgaccccgcg atgatcatcg ccggccgaag ctccaacgcg gtgcggcgaa
      781 gcgcgtcgag atcgatcgtc cctgatacct cgtcgaggcc gaagagcgtg acgtgatagt
      841 cccgtccgct caaatgatag gaggcgccgt ggctgtgatg gccgccggcc gcctcgtcga
      901 gcgcgaggac gcgcgcgccc ggctccagaa gcccgcgcag gaccgcgatg ttggcgatcg
      961 tcgagctcag cgcctggata ttggcatgct cgccgccgaa gagagaaagc gcgcgcgcct
     1021 gcgccagccg ctcgatctcg tccatgatct cgcaaccggc cacggcgcgg cggcccggat
     1081 agcccgtcgc gttcttggat acgagaatcg aggactccgc ctcgagcgtc gcgcgcgggc
     1141 aatagctctc cgacgcgatc aggttgatcg tctcgttctg acggcgcctc tcctgtacga
     1201 caagacgaga gagctcggga tccagcacat cgaggccgtc ctggagggtt gctcccaccc
     1261 cgaccgcgtt cgcgtgcggt gccgcgatag ttgtgttgct cggtctcatg cccggttctc
     1321 agtgaacata ggctgtgcgc ttacgctcga agcgacgccc cagccaagtg aaggggtaac
     1381 aaatgacgaa gtacatgacg aaggcgatgc cgaagacctc aaaggtgtcg agcgtgcggt
     1441 tggcgatctc cttgctggcg agcgtgagct cccaaatgct gatcaccgag gcaagcgccg
     1501 tcgccttcac ggtgacgggg atcaggctga catagggcgg caatgcgaca cggagtgcct
     1561 gcggcagcag cacttcgcgc atcagcatga gcggcgtcat gccgagagcg agcccgcctt
     1621 ccgcctggga gcgcggcaag gcgagcagcg atccgcggaa gacttcggtc atgagcgcgc
     1681 ccatatgcag cgagatcgcg atgaccgcca cgacataggg cgacacccgg atcccgaaga
     1741 acggcagacc gaaatagacc aggaacagct gaaccaggat cggcacgccg cgcagcacgt
     1801 aaacgagcgc cagcaccacc gcttgtgcga tcgtgccgaa catgacgctg accaccccgc
     1861 cgacaaggcc cacggccgtc gaaatcacga tcacgacggc cgagagcatg acggtgcgcg
     1921 tcgcggcctc gatgagatag ggcaagctct ggaccacgct ctccatgagc gatctcctac
     1981 ttcacgattc ggcagccctg ctcaaccacg cggagcagga gcgagaaaat ggtcgagagc
     2041 acgaggtaga tcaggccgac cgcgacgaac atttcgaagc tggcggcgct tcgctcggcc
     2101 agcgtgcggc cctgcagcgt gatctcgtac accccgatcg tggaaaccag cgccgtgtcc
     2161 tgcagcagca ggatgacctg attgcccatc gccggaacgg cattgcggag ggcctgaggc
     2221 aggacgacgt aacgcatcag ctggcccggc accatgccga gcgcgtaacc ggcctcgcgc
     2281 tgccccgacg cgaccgactg aatcgtgccg cgaaagatct cggcaaaaaa ggcgctgtgc
     2341 tgtgccgcaa gggccgcaac accggtctcg aacgcatcca ggcgtatccc cagggacggc
     2401 agggcgaaaa aagcgaagta gagctggatg agcagcggcg tgttgcggaa gaactcaatg
     2461 taagcggccg ccgccccgcg caacgggccc ttcgacaacc ggccgatggc cagcagaatg
     2521 ccccaggcga atgcaatcgc gagagcaagc aacagcaact ccagcgcggt caggatggcc
     2581 ggcccgaagt caggtagatg acggatgacg taatcgatgt cgaagcgcat atgggcgttc
     2641 acggcagcgc ctccgcactc tgccccccat tgccgctcat gcgctccgtg atcaggcgca
     2701 tctcctcgaa ccgacgtagg aactcgcgca gacgcggcgc ctgtggattg aacaagagct
     2761 cggccggcgg cccttgctcc aggatgcggc cttcatccat gaagatgacc cgatcggcga
     2821 catcgagcgc gaaggggatc tcgtgagtga ccacgatcat ggtcatgcca tcctgcgcca
     2881 accgacgcat gacgttcagg acctcgccca cgagctccgg gtcgagcgcc gacgtgacct
     2941 cgtcgaacaa catgacctcc ggctccatgg caagcgcccg ggcgatcgcc acacgctgct
     3001 gctgtccacc cgagagggtt gccggataga cccgtgcctt gtcttcgagg ccgacgcgcc
     3061 gcaacagctc catcgcaagc gattcggcgt ccgcacggga cagcggctcc gccttgatcc
     3121 gcgtcggacc gagaatgatg ttctcgagaa ccgtgcggtg cgggaacagg ttgaaggcct
     3181 ggaagaccat accgatgcgg gttctgtcgc gtgcgatctc atggatcggc cgctttttcc
     3241 gacactccgc gtcgcgatac cacatcagct tgtcgccgat atggatctgg ccggcttcgt
     3301 actcggcgag aaaattgagg cagcggagaa aggtggtctt gccggagccg ctcgagccga
     3361 tcaggaccac gacctctccc ttctcgacat cgaggtcgac gccgcgcagc acctccagcg
     3421 gtccgaatct cttgcggaga ctacgaatcc gtatcatggg cccgccctcg tcgtgtccgt
     3481 ttctcgactg gggcggctcg cgtacgagcc gccctgactt gagtcatttg ctcgcagcga
     3541 aagcgataca gcggtccggt gagggcgggt cgacattgaa ccacttgttg tagatcgcct
     3601 tgtactcctc gtagagcgat ccgcaccgca tttcctgaac cagcgcgtcg aggtagtacc
     3661 accactgaaa atcgttctgg cgcatgaaga tggcgttttg ggtgctcgac cctacgatcc
     3721 cgagattctt tatgtccggg atgttgcgca cgcgccaagc ggcgacgggc tggtcgatga
     3781 gcagggcatc ggcgcgattg gttcgcaccg ccgtcagcat gtcggcttcg gacggaagga
     3841 ccaacgtctt cgcctccggc agcttttcgg ccatcagctt gtgctcgctc ggggtgtcga
     3901 ggatggcgac cgtataatcc gagttgttga aatcctggat ttcatccgcc tcgatatcgg
     3961 tgcgcgtgag aagcgacagg ccggagcgga tgtacccgcg cgtgaagttc acccgcgcga
     4021 gacgatccgg ccagatcgtc gtcaccatga tgccgaaatc gacctcgccg ttgttgacgg
     4081 cggcccaccg cgcgggaaag gcgagtttct tgaactcgat cttgtcggga tcctcgaaaa
     4141 gcgctttcgc gatcaaccgc gcgatgtcga tgtcgaaacc gacgagttcg ccgttctcgc
     4201 cgacggagcc gaacggcggc acctcggatg tgacgccgac gatcacgtgc ccccggtcga
     4261 gaacctcgta aagcttgctt gtttgttgcg ccatggcttg gccggccgca aacgccattg
     4321 ctgttgccgc cccgatcgcc cagatcatgc ttcgtattct tggtttcatt ggccttcctc
     4381 ccttgttggt gcagcggagc ggagctatcg atccggttcc gcctcctcga acagccggcg
     4441 cagatgctcg ccggcgattc ccgccccctt tgccagggcg agctggatgc gggcctttgg
     4501 cccttgcagg ttggcgaaaa tcacaccggc atcgcgcaga tcgcgctcat cgccggttac
     4561 ccgttgcggt tgctcgtaat gatcgcggat gagtcggccg gccgggctcc gaggcgtggc
     4621 gacaatgccg attccagctg ccattgcctc ccggactgcg ggcatcagga ccggggggac
     4681 ctgtccggat cccagagtct cgatcacgag gccatccgga gccgcggcaa ttgccgcccg
     4741 gacgaaacag tcactcatcc ctgcgtaaca tttgacggcc tcgacatagc cgacccgccg
     4801 cgtcgtcggg agtatggttc gcctagccgg gcgcctggca acgaagatgc gatcctcgtc
     4861 aatgctcccc agcggtccaa attccgggct cttgaacgct ccgagactcg tactgtgaaa
     4921 cttggtgaca tcccgagccc catgaatctc cgcctccatg acaacgaccg ctccgaggcc
     4981 gcacagttgc ggcgaagccg cggccaggaa cgcatgccgg agattgcgta ctccatccgt
     5041 tccggcgacg ccgtcgggca gcatcgcggc ggtcagcacc accggccgct catcatcgtg
     5101 ctgcaaatcg acgaagaagg cggtttcctc gagagtgtcg gtgccgtgtg tgacgacaac
     5161 ggcatcggcg ccgttgcgga acgccgcggc aaccgcctct gcgatgcgca gcatttccgg
     5221 aagaccgatg aacgtgctcg gtagatcaaa cagattttcg acgtgaactt cccccgccac
     5281 gccggcgctc gcgagcagat cctcgccgga cgcatgcagg catcccgttg ccggattatc
     5341 gcgctcgccg atcgttccgc cggtgctcaa caggacgatg cggctcgcgg cgttcatgac
     5401 gatgtcttct gcaagcttgg atatttgcgg tagtgcttgc ggacggctgc ttgcgccttc
     5461 cgtcggctac gctcccggat ggcatcgacc atcgccttgt ggatgccgaa ggactcatcg
     5521 gcgatcgacg gggagtaatt ctgctcgatg atccagaggt tcggccgaat gagatcgatc
     5581 agatagcaga cgacgcccgc caggacggag ttgccgccgg cgcgggcgat cgcggcatgg
     5641 aggtcgagcg taagcggaag ataccggtgg acatccccac catcgatggc cccgcgcatt
     5701 cgccccaaga tcccgtccat gacctcaagg tcatccgctt tcgcgttctc gatggccaga
     5761 ttggtaaccg cgagatccag cgcctcacgc gcctcgatgg cctcgatgac attggcgctg
     5821 gcatcgaggg tttgccgtag ggaaaccgcc ttcagctgca aggggtcgat gctctcggca
     5881 agataagtcc cgtcgccgac atgccgctcg acgatgccgg ccatctggag cgcattgagc
     5941 gcctcgcgca cgacgcctcg gctgaccttg aacgtctcgg ccaggatccg ctccggcggc
     6001 agcttgcgct cgctcagaag agtgccaccc gccatctgag ccagcaggcg ctccgccacc
     6061 ttcagactct tgcggccgat cagagcatgc gatcgcagtt gcatggtccg agaggttaga
     6121 ttggtccgac caatcatgca acacgaaact ccacgatcgc agttaggcgc ggcacgaggg
     6181 tctgctagcg gagaggccgg gatcgagaga gcagacgcat taactcatgg cgagatagcg
     6241 gcgagcgacg ctcttaccaa gcgctttccc aagaatgaac ccaagcttgc gcagcttgtt
     6301 gccgcctcat ccaaggccgc cgagaccgtc gcacttgagc agcatctgat gcccagttca
     6361 cccgacaatc actccgccga ttcccggggg cgtcggcgtc atcagaatgc tcgcccacgg
     6421 cagagatgcg agccttggcg ggatattcca gttcaccacg attcgctcga cgcatgcgcc
     6481 actccgcgcc gcttggatcg gagggatagg atcgatacgg catccgggac aggtaggcgc
     6541 acccggttgc cattgcgccc catgccgccg cgcttaggca gactctcagc gcgcgcccaa
     6601 acgctcgcgc aatgacattt caaagcagcc ctttacattg agggcggaga gaaatggcgc
     6661 cccaaaacca ctcggctact ctgcgatgga gcggccggtt cgttctgcca ggcaacgtat
     6721 gcgcactgcg atatgtcacc agatcgcgtc gttcagctcc accttgtaac catcgggatc
     6781 ttcgacgaaa gcgatgacgc ggctgccgtg tttcatcggg ccggcaggtc ggacaatctt
     6841 taccccattt ctggcaagcc gttcgcacgt ggcataaacg tccggcactt cgatggcgat
     6901 atggccgaag gcttcgcccg tcggataagg ttccgaacgg tcccaattat aagtcagttc
     6961 gatcgtcgta tggtccactt ccgccccata gccgatgaag gtgttggtga acttgccgtc
     7021 cggatattcg gtctgccgca acactttcat gccgagcagg cccgtataga aatcgagcga
     7081 ttgtttcagg tcgcgcgtgc gcagcatcgt atgcgacagg cggtattgcg tgatgtcgtc
     7141 agcccttttt ccggcctgcg tgtcagttgt cattgtcgtt actcctattt gcggtcgatg
     7201 cggaattcga gaagccgcca ccggtcatca tctccgccaa gcgcagcaga tcgaggtcac
     7261 ggccggccgc gccgatgatc gacagtccca gcggacaacc ttccagcgtg acgagcggca
     7321 gggttatttg cggcacaccc gaaaggcttg agatcgccgt caacccgagg acgcgatcgc
     7381 gataattgct caattcctcg gccgtcgccg ttgtcaaagg cgcgatgccc ggtgcagtcg
     7441 gtaagagcag aagacgattg ccggacacca gcttgtgaat gctgtcggca aggtcgtcct
     7501 ggacctcgcc tgcctcggct tctgccgctg cggtcaagcc gcgtgcccag gcgaaacgcc
     7561 cctgcgcttc gggaccgaga cgcggtcggg ccgtttctat ccacgcccca tgctgacgcc
     7621 agatctccct gccctgcaag atgcggcagg acgggatgag tttggcgaaa ctgccagccg
     7681 gttgtgcaac atcaatggca cgcgctcttc cgaagcgagc ttcgaatgtc aggatatggg
     7741 gcgtgaggac agcccggacg gccggatcga gcagcccgaa ggcatcttcc gccaggagca
     7801 attccgaaac ggcgatatca tcctttgctg tcacgccgta cagaacttcg ccgacctggc
     7861 gcagcacgaa ggccgtgcgc gcgaaccatc caacggtatc gcagaacggc gatgcgggca
     7921 cgaggccttt gacggaaatg cgcccatgag tcggccgcat gccgaacagc ccgcaatagc
     7981 tggccggaat gcgaaccgag ccgccggtgt cgctgccaag tgcaaaatcg acgataccgc
     8041 cagcaacggc cgaggctgat ccgcttgaag acccgcccgg aatgcgccca ggcgcagcgg
     8101 gattgaccgg tgtgccgtaa tgcggattct ggccggcgag gctataggcc aattcatcgg
     8161 tgatggtctt gcccgccaga tcggcccctg caccgagcag tttcgcaacc gcatccgcat
     8221 gcgccgctgc gggaggatgt gtgcgcgccc agtccggatt gccgcagccc gtcacgaaac
     8281 cggcaacgtc gaaattgtcc tttgcggcaa aaagcaagcc cttcaaaggc ccctctgccg
     8341 ccccggccag tctcacctct gtgccgggaa cgaaggcacc gagatccgct acgctgcaat
     8401 ccgccgttga aagcaaaccg atctatccgt tacctgcagc tgggatgatc cagacatctt
     8461 ccggccgcca ggatgcgaaa actgtgctgc ttggctcgat cagcggatcg ccgatattgg
     8521 tccggttcgc cagcaatatc tcgccgttat cgagcctgat ttcatagcgc tgcgtcgtgc
     8581 cgaagtaatt ggcatcgacc acccggcctt gatagccggt attgtcgggc acctgagtaa
     8641 gcgtcacggc aacgcgttcc ggccgcaccg ccagggtcac gcgactgccg acgcaagcct
     8701 gtgtccttcc ggatgttttg acgacggttc catgctcggt ccgcgcaacc gagatatcac
     8761 cagtcgacac caatgtcgcg tcgagaaaat tcgccgagcc gaggaaacct gcgacaaacc
     8821 gcgtgcgtgg cgtttcatac aattctttgg gcgagcccac ctgctcgatc tgaccgtcct
     8881 tcatgacgac aactcgatcg gacatgctaa gcgcttcgtc ctgatcatgg gtgacataaa
     8941 ttaccgtact gcggatcttc cgctgaatgc gtttgatttc gccttgcagt tcctcgcgca
     9001 gcttcttgtc caacgctccc agcggttcat ccatcagtag taaaggtggt tcgaagacca
     9061 gcgccctggc caaagccacg cgctgctgtt gcccgccgct gagctgtagc ggatagcggt
     9121 ccgccatgtc ctgaagctgg acaagttcta atgcctgccg cacgcgggcg cgaatggctt
     9181 ccttgccgag acgacgcatg cgcaaaggaa aggcgacatt cttgaagacc gtcatatgcg
     9241 ggaacagcgc atagttctgg taaaccatgc cgatattgcg ccgatgcggc ggcaatcggg
     9301 tgatatcctg attccccagc aggatcgcac cttcggtcgg aataatgaaa cccgcgacca
     9361 tcaataagga tgtcgtctta ccggaaccgc tcggccccag gaaactgacg aactcgcctg
     9421 cttcgattgc cagcgatagg cgggaaacgg cggtgaatgt accgtagcgc ttgaccacat
     9481 tggaaagggc gatgctggcg cccacccgat ggactgccga aacttcaggc ttcgtcgcgc
     9541 ttcgattgtt cgtctgcgga aacctgccca aggacaattt ctctgtcgaa cgccgcgacg
     9601 agagactttc cgccatcaca atgttccttg ttcgcttgag gcatgtctat tgacgatcat
     9661 cattccggtc ctatctccgc gcgctgacct tcacccgccc catcagaaaa tgacggagca
     9721 actcggcggc aagtacgatg accgacgacg tcacgatcag acatgtggag acggccgcga
     9781 tcgtgggttc cagttcgaaa cgaacgccat cccaaaggcg gcgcgacaat gtcatcgtct
     9841 ccgggccgct gacgaatatc gtgataacca cctcgtccag cgacagtagg aaggcgaaca
     9901 ggaacgacac gatcaccgcc ggccggataa tcggcagcga gacttcgaaa aacgcccgaa
     9961 ctctcgacgc gcccagaccg acggcggcat cctccaaccg ctgatcgaac tccgccagcg
    10021 ccgacgagat gacgactgtg gcataaggat aggtgacgac ggtatggctg agaatgagcg
    10081 cccacaaatt cccagcccaa ccgatctgca gtataaagta ataaatcgcg acagccgtga
    10141 cgataaccgg cacgattaac ggcgccagaa tgaccgcgcg caacgcgttg cggccgatga
    10201 agcgaaagcg caccagaacg atcgccgagg tcaggccgag agaggtcgcc aggatcgaga
    10261 ctgcgaccgc aaccttgaaa ctgttccagg tcgcaaccat ccaatccggg ctcgacagat
    10321 atttctcgaa ccaacgggtc gaccaatgct ccggcggaaa ctgcagcaag gtcgaatcgc
    10381 tgaaagcggc cggaattacc accagaagcg gggttattag gaacagaagg acacaggcga
    10441 cgtaaaacca cgtgccccag cggagcagcg taaatatatc gcggctccac ttcactttgc
    10501 cctccggtct tgggcatcac gttgaaagga gtagtagagg atgaggagcg ccgccgtgaa
    10561 gatcaggagc agggtggcca gagcggagcc gaatccccag ttgagcaatt ccgagaaata
    10621 ggagctgatg agcatggcga cggtgatatc accgcggccg ccgagcaaag ccggcgttac
    10681 gaaaaagccg agcgcgatga cgaaaaccag aactgccgag gccttaagtg ccggaaggct
    10741 taaggggaag aagacattca cgaacgcacg caaggggtcc gctcccaatc ccttggccgc
    10801 attcagaagc tgaagatcga ttcccttcat cgaactgtat agcatcagcg tgttgatcgg
    10861 gatgaggtaa tgtgtcatgc cgatgagcac gccggtcgtg ttgaacacaa gctcggccgg
    10921 cgcagagagg atgcccgacg acgtcaaaac cgaattcacc atgcctttcg tctgcaggac
    10981 caacatccag gcgtagcagc ggatcaaaat gtttgtccag aacggcaaca ggatcaggcc
    11041 cagcatgagg gcccgcagac gggttggcgc aatgatcagc gcgaaagcaa gcgggtagct
    11101 cgcaagcagg ctcagtgccg tcgaaaccag tgcgatttcg aagctgacct tgaagactga
    11161 taaataaacc gggctcgaga aagccttccg gtagaagtca aaggtgaagt ccggatcgaa
    11221 gaagctctgt tgcagcagta gggcaacggg aatgatgaaa cagatgatga ggaaaaccgc
    11281 acccggcgtg atcagcgtga caaatccaag ccgagcaagc agaaaatctg tcaatcccga
    11341 actctgacga aggctgtacg aattctctgc ttgcgcgtgc atggatgctt ttctcacgac
    11401 ggggaaatca atgagccatc acctcatcga tcctatatga gctgccagaa attccagcgc
    11461 tcgaccagtt cctgctccct ggtcgaccac caatccgcgt tgaactcgat gatttggccg
    11521 gtctggtagt aatagctcgg cagtgtcttc ttggtcgcgt cgtcgaggga atccagcgcc
    11581 ttgaaattgg aggggccaac gccagtcgcc ttggcatagt ccgcctggcg cttcggatcg
    11641 agcatccagg aaattagctt ctgtgcattt ttcttacgct tggatccctt tggaatggac
    11701 aggaagtcag tcgtcagaat gccctgtgac cagacaatct tgatcggcgt atccggctct
    11761 tcctcgagca acgacataac gcgaccatgc gatgtcatgg tgacggcgag ttcacctgaa
    11821 gctagcagat tgatcgattg cgggaactta tcccaccaca gaacatcgtt cttgatctta
    11881 tcccaatgtt ccagcgcccc ttcgacatcg agcggataga gatccttagg cggcacgcct
    11941 ttcgccatca aggcgatctc gagcgcgtaa cgcggcgcgt cgatcatgcc gcgtttaccg
    12001 acaaatttct cgaaatccca gtaatcggcc cagctagctg gcgcctgctt accttcgaaa
    12061 gccttggtat tataggcaag gatcatcgcg aaagtcgcaa caccggcgcc atagggcctc
    12121 agcagatcct tgggcacgcc ctccgattgg atctcggaat agtcgatctc ctcccacagg
    12181 gaggccgcct tatccttatc ggccacccac atatcgagcg cttggacaac atcccacgag
    12241 acattgccgg aatcgaccat agttttgact ttggccagat cgagatcggg cacatcaacg
    12301 acacgaatgc ccgtttccct ctcgaaatct ttgaaaaaat actcgcgttc ggcctcctgc
    12361 cagttcccac cccagcccgc aaaaacgacg gtgtcatcgt ttgctttagc cgatttcatc
    12421 gaagtcaagc caagcggaat tcccaacgcc gcggcaccca ctgcagacgt aaaggctcgc
    12481 cttgtcattt ttacggaatt cttcatttct tcctccacaa aactaattgt tatttctgtt
    12541 tgcttttttg ttacgcgccg aaattcatcc gcacgaattc ggtttcaccg tatgacctga
    12601 ctcccctgtg acgcgaccgg aattcgctcg ttcccaggct atagaatgaa ggtcgtgttg
    12661 ccttgcggtt cttccgaatt gatcaagctg atgatcttga attgggtacg gaagcgcccg
    12721 tcccgaaatt caagcctatg atcctgccgg ccggcatagg tggtgatgcg accaagccga
    12781 tactccgaga tcaggatatt cgacctgacg ccgacgaagc catcgtcgtc ccgccatgcc
    12841 atcacattgg tcaccgcgcg cgcggttcgc gaagccggga gctgcgccac ctggacgccg
    12901 gtttccatga gcgtgattcg gtcgagcaag cgccgcctat catcgaaatt gatggccact
    12961 tcgtgtcttg gatcctgcgg atcgcgtgtg ctgggcagcc agtagacaaa ggcatcgctg
    13021 aacaactcca gccaatcccg ataacgccga tcatcgagca aggctgcttc gaagtagatg
    13081 aacttctcca cgtttctcag ctcctgatcg tccaaattcg gcccttctga ccgtgttctt
    13141 gcgcgacgca gaaaggcgtg gagggaatca taaagcccat catctacata agagatttcc
    13201 tcgccgggcg cgccagcgct tccatggacg gccatggagt cgcgcgcttt ttctgcaatt
    13261 ggtacgatcg gcatatcaac tccgtgttga cttcaaggct ggtattccgg caacccgacc
    13321 agccgatgaa tgctcatcga aaaacaaggc gaaacgcagc taacgcaccg ccgtgcgagg
    13381 ctctctattc atgagcgtca gccaatggct ataggcgccc ctaatcgcgg tatcgtcggt
    13441 cttgttgctg gtaatgaccc cattcggcga gacggtctga cgattcatgc cgcgcgtcag
    13501 atcgagccat tccatttcag ggatggtttc gagcgcatgc tgaacacgct ccatgatgtc
    13561 gctatcgtcg cgcgacccga cattgttgaa atcctcctcc gacctcaccc ggagtctgtt
    13621 gacctgttcc ggcacatgag ctggaagaat cgtgtaatat cggactttcg tttcattcac
    13681 ggacactggt tcgaagactg caaaggcgcc gttccccatg atgaagaggt tgggatagag
    13741 gatcagattg attcctgccc tgccagtcag ttcgagataa tccttcgccg cctcttcccc
    13801 gactgccttc cgcaaagtcg cggcttccga ttcccggccg gggaccggcc tggcctgctc
    13861 ccaggaggag cgcaattccg cacgatgatc gcccaattta tggccgttcc ctaaatactg
    13921 gcagatcatc gaggtattat ctttgacgtg ggacagccat ttgccagaac catggcgccg
    13981 ctggttcatg atattggtgg ctttatgcaa gaacggcgta tggtagccgt cggtgttatt
    14041 gtcgttctga tgcttccaat tcccccgata catcatctgg gtgcccttga tgacgcgcac
    14101 ctcatccggc ctttcgcaag ccaagccgtc tttgtcgatg ctccaatcga ggatttctcg
    14161 cgccggcccc atccagtcga tcagcggcac ctcatccgga ttcaggcagc cgaagacaaa
    14221 accgcgatag ctttccacct tcggaaaacg tccgagattg tgagccgcgc ggtcgaagga
    14281 cgggccgtag ccatcgggga acgacgccga cagcatttcg ccggtgcatg aaaatgtcca
    14341 agcgtgatac gggcaggtaa agcgcggcgc attcccttgc tcctgcagac agacagggct
    14401 gccccggtgc gtgcagcgat tgaggaaggc ggtgatcttg ccatcgggcg cgcgcgtcac
    14461 gacggtcgaa cgcaatccca ccgtgaccac cttgaaatcg tgcggattgg gaatctccgc
    14521 ttcatgcagc aagaagaccc aggttccgcc gaatatcttg ttcatctcct cccgaaagat
    14581 ctcgggatca gtgaacaaca ggcggtgaat catgccgtcc ggccgaacca gatgatcgta
    14641 cttgccgtgc tttgggctgg attcgggcct gtttagcggc acctgcatta cattctccca
    14701 tttcagaggc tcttgcgttg agcgctttcc ttatcttgaa caaactagag cttgtttgaa
    14761 aatttgtcaa gctcttgaat aacccagatt gagcgctcaa cccttgtcat ctcgccggtg
    14821 atcccggtat tttgagatgg ccttacaaaa tagcgacggc atccatttgg agcccgacat
    14881 catttccagg caggcctgtc acgcgcgcca ccgtgacggc gggtgcgttt gggcccaacc
    14941 gtgccgtcag ggcagcaccc agttccggca gaattcggtc gacatcatca ccgtccaagc
    15001 cgggggagag aaagaccgtc agcttggcca gtctttcggg tgaaacctgg agaccagcca
    15061 aagcattcaa cacctgaccg agcgcatcga gcgcgtcttg acgaagtccg tgggtccgag
    15121 ccgggctctg gccgctcaca tagacgagat tgcctctttt cgccgcctgc tttcgggccg
    15181 gccaattggc gttctcggtt gccccttcgg cgatgctcga cctaccaccg atcaccgcgg
    15241 tcgcatcgat ctccagcatc aggccctcgt aaagaagatt cacaccaaat gccgttgtcg
    15301 gcgtgtgaac agccgtcgga cagacctcat cgaccacgcg catgatggtc tcgagtcccc
    15361 gcttggcctc agccgaatca ccgcgatgca tatagcaaat cttgaggtga atcagatccg
    15421 cgaaactgcc gccgctgtcg cgaagaacgt tcgcgatatg catcatcaca ttgcgggtct
    15481 gcccgtcgat atcgccgagc gcgacagcac gtccggcgcg gtcggcggca atctgcccac
    15541 cgacccaaac atggtcgtca atgcgccatc cctgtgagag cggaatcgga atgctccaat
    15601 cccagctttc atccggcatg atccgcttgc gttcccgccc cttctccggc gcaacggcct
    15661 ctgcttcgat ctgaataagc gcaccctcga gcggtatgcc gcgcacccgg acggcggtgc
    15721 cgacaggccc aggatcgggg aagtattgca ggcgcacgcg tgtcattttc tcccagtagc
    15781 gggtggcttg gtcttcggca ccgtcgtaaa cgtaataggt gttgatgcgc accaggtctt
    15841 ccatacggca acccattccc gccagggtcc ggcgcatgtt ctgaaagacg gcatgcgtct
    15901 gcgcttcgat atcgccgggt tcgacgacct cgccgcgctc atttagcggc agcacgccgc
    15961 cgacaattgt ccgcgatccc acccgcaccg ctttcggaaa tggcaaagct tccttgaagg
    16021 tccacccaac ctctgaagtc aactttgtta tttgcatgat cggaagctcc caatgaaaac
    16081 gaaacgagtg gaaggggaaa gctcgcggtg acgctgacta cccgtcgacg ctttccaatg
    16141 atgtcagggt cctgctcccc tccggtccac accagtcggc gagaatatcg gtatagatgg
    16201 cgaccgattg ctcgatccgg tcgacccggc aatactcatt cgtttgatgc gccatatgcg
    16261 gctcgccggg gccgaggatc accaccggcg catcgggata agcatgctta agcgcgcagg
    16321 catcggtgaa aaagctggcc ccgcgcggct caggcgaaac gcccagatgc cggccgacga
    16381 tcttataaac tttctgcacc caggcatccg aggcatcggt ccagatatgc tgcagatcgg
    16441 ccgtcgtctc gatttccgcg atatccggcg ccagataggt cagcagttcg tcgcgaactc
    16501 ggccatgact catgcctggc actgtgcgga catcgatctc gacttcggcc ctgtccggca
    16561 cgatattgac gctcgatccg ccgcgaaatg tgccgacatt gatcgtcgga ctacctagag
    16621 tcgcgtggcc ggcgatattg aagccgaagg cctccatctt gctggctaac cgtgctccac
    16681 gatagatcgc attgatgccc ttttccggca tggagccgtg ggcgccgacg ccggttgccg
    16741 aagccttcag ccagagcgca cccttatgtc ctatgagcgg atagttcgcg gtcggttcag
    16801 ccacgaccac ggcgccggcc tcgcccagca cgtttcccaa cttcacgagg tgaaaggctc
    16861 cctggcagcc agtctcttcg ctggcggtca gcaccagcac gatgccggga ccgttgcgca
    16921 gcatgtccag ttgcgcgatg gccgccacca cgaaggcggc aatgccggcc ttcatgtccg
    16981 acgaccctct gccatacaac cggccgcctt tgatctgacc gccgcaagga tcgtcattcc
    17041 aagcagcgct gccgataggg accgtatcca gatgtccggt aaagcagatt ggcttccctt
    17101 gaccgctcga gcgatagcca gctaagcgcg cgatgacact cgtgcggccc ggcgagaact
    17161 cgtgatattc cacttggaat cccgcttccg ccaacagcgc gccgagatag gccgcacagg
    17221 cctgttcaga gcctggtgga ttgtatgtcg gaatgcggat gagttgttgc gcgagcttta
    17281 cggcttcgtc ccgttccatc attaccctgt ttttctttat gaatgtggcg ctgatcagca
    17341 cgacggcgag acaggcgcaa caacaatcgt tgacttagct tgagctcaca ctgacattta
    17401 gtcaagtact tgaatcgagc caaactgact ctggtcgtcc tcccttatcc tgccgcccag
    17461 aacggaattg caacctccac accataacgc gagacgccgt aacgaatctg ccggtgcgct
    17521 tagatgtacg cttcgatatc
//
LOCUS       DASVDU010000130        11192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_17260, whole genome shotgun sequence.
ACCESSION   DASVDU010000130 DASVDU010000000
VERSION     DASVDU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17260"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3031
                     /gene="rpoB"
                     /locus_tag="VF226_08285"
     CDS             <1..3031
                     /gene="rpoB"
                     /locus_tag="VF226_08285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7074023.1"
                     /translation="YRVMRPGEPPTVEAAETMFHGLFFDPERYDLSAVGRVKMNMRLD
                     LDAPDTVRVLRKEDILAVVKTLVDLRDGKGEIDDIDHLGNRRVRSVGELMENQYRVGL
                     LRMERAIKERMSSVDIDTVMPQDLINAKPAAAAVREFFGSSQLSQFMDQTNPLSEITH
                     KRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPNIGLINSLATFARVNKY
                     GFIESPYRKVVNGKLTDEVVYLSAMEEMRHHVAQANAEVDEEGRLVGDLVTCRYQGDV
                     ILVSPDRVDYIDVSPKQLVSVAAALIPFLENDDANRALMGSNMQRQAVPLIKAEAPLV
                     GTGMEPIVARDSGAAIAARRTGVVDQVDATRIVIRATEETDPSKPGVDIYRLRKFQRS
                     NQNTCINQRPLVNVGDTVQAGDIIADGPSTDLGDLALGKNVLVAFMPWMGYNFEDSIL
                     LSERVVSEDVFTSIHIEEFEVMARDTKLGPEEITRDIPNVSEEALKNLDEAGIVYIGA
                     EVNPGDILVGKITPKGESPMTPEEKLLRAIFGEKASDVRDTSLRLPPGVSGTVVEVRV
                     FNRHGVEKDERAMAIEREEIERLAKDRDDELQILDRNVYGRLKDALLGKEVAKGPKGA
                     RKGTEIDEAVLADIPRSQWWEIGLKDEKAQAEVEAIQKQYEEAKKSLERRFVDKVDKL
                     QRGDELPPGVMKMVKVFVAVKRKIQPGDKMAGRHGNKGVVSMIVPVEDMPFLEDGTPV
                     DVVLNPLGVPSRMNVGQILETHLGWACRGLGRIIDEALEQYRESGQLDRLKETIEKVY
                     GKADGVSKLTNEELVALGEQLRVGVPVASPVFDGARETDINEMLQLAGFDTTGQVTLY
                     DGRTGEPFDRKVTVGYKYLLKLHHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQRFG
                     EMEVWALEAYGAAYTLQEMLTVKSDDVAGRTKVYEAIVRGDDTFEAGIPESFNVLVKE
                     MRALGLNVDLINSEAPALEEAEEEQPVRPQLPSPAAE"
     gene            3210..7430
                     /gene="rpoC"
                     /locus_tag="VF226_08290"
     CDS             3210..7430
                     /gene="rpoC"
                     /locus_tag="VF226_08290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7074024.1"
                     /translation="MNEISNIFKQQQVLPTFDQIKISIASPETILSWSFGEIKKPETI
                     NYRTFKPERDGLFCARIFGPIKDYECLCGKYKRMKYKGLICEKCGVEVTLAKVRRERM
                     GHIELAAPVAHIWFLKSLPSRIGLLLDMSLKDLERVLYFENYIVIEPGVSTFREKQLI
                     TEEQYNQAIEEFGPDSFTAGIGAEAIRQLLASLDLHKIRDELRQEIQESTTELKPKKL
                     AKRLKVIEAFIESGNRPEWMIMTVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLMELRAPDIIIRNEKRMLQEAVDALFDNGRRGRVITGANKRPLKSLADMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYARLQTLGQA
                     ATVKQAKKLVEKEKPEVWDVLDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLSLEAQLEARVLMMSTNNILHPANGSPIIVPSQDIV
                     LGLYYLSMVRDGEPGEGMILGSAAEARHALEAGTVTLHAKVKGRFRSLDENGNEVVQL
                     YDTTPGRMILGELLPQQPGVKFELVNQLLTKKAISSMIDAVYRNCGQKETVIFCDRIM
                     QLGFYHAFKAGISFGKDDMVVPDSKARIVEETTELVREFEQQYNDGLITQLEKYNKVV
                     DAWSKCTDQIAKEMMDHISAVRKDSNGRELPINSIYMMSHSGARGSPAQMKQLAGMRG
                     LMTKPSGEIIETPIISNFKEGLSVLEYFNSTHGARKGLADTALKTANSGYLTRRLVDV
                     AQDSIIVEEDCGTDAGINVQAVVDAGQVVVSLGNRVLGRTAAEDIIDPATGEVIVPTG
                     KLIEEREAEAIERAQIQEVRIRSVLTCETVTGVCAKCYGRDLARGTPVNIGEAVGVIA
                     AQSIGEPGTQLTMRTFHIGGTAQVVDTSFIESNFNGTVRIKNRNVVRDSQGRLISMGR
                     SMSICIVDQSGRELATHKITYGARLLVDEGDTVKRGTRLAQWDPYTRPILTEVDGTVD
                     FEDLVEGASMREQTDEVKGTTNRVVIDWRASPRGADLKPAIVIKDENGKPIKVARGGD
                     ARYLLPVDAILSVEPGAQVKAGDVLARIPMASARSGDITGGLPRVAELFEARRPKDHA
                     IIAEISGTVEFGKDYKNKQRITIRPDDEEQPPVEYLIPRGKSLAVQHGDRIERGDFVY
                     DGHPAPHDILAIKGVEELANYLINEIQDVYRLQGVTINDKHIEVIVRQMLQKVEVTDP
                     GDTTFIKGEQIDRIELDEVNAKIEKQGGRPATAQPILLGITKASLQTRSFISAASFQE
                     TTRVLTEAAVNGKVDTLEGLKENVIVGRLIPAGTGGMLRRLRRIAAKRDELIARERAK
                     ADSERALAGPDLEMPRERRRRRAEEAAE"
     gene            7907..8278
                     /gene="rpsL"
                     /locus_tag="VF226_08295"
     CDS             7907..8278
                     /gene="rpsL"
                     /locus_tag="VF226_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX7074025.1"
                     /translation="MPTIQQLVRKPRQQKVYREKSRHMEGSPQKRGVCTRVYTTTPKK
                     PNSALRKVAKIRLTNGYEVIGYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGVL
                     DTQGVADRKQRRSKYGAKRPK"
     gene            8395..8865
                     /gene="rpsG"
                     /locus_tag="VF226_08300"
     CDS             8395..8865
                     /gene="rpsG"
                     /locus_tag="VF226_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX7074026.1"
                     /translation="MSRRHRAEKREINPDPKYGDQVITKFMNAVMNQGKKSVAERIVY
                     GALERMESRAKQDPVQLFRQALENVMPAVEVRSRRVGGATYQVPVEVRADRRQALAIR
                     WIITAARARNENTMVERLSGELLDAANNRGTAVKKREDTHKMAEANRAFSHYRW"
     gene            8887..10962
                     /gene="fusA"
                     /locus_tag="VF226_08305"
     CDS             8887..10962
                     /gene="fusA"
                     /locus_tag="VF226_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7074027.1"
                     /translation="MPRSHKIEDYRNFGIMAHIDAGKTTTTERILYFTGKSYKIGETH
                     EGSATMDFMEQEQERGITIASAATTCFWKGKRLNIIDTPGHVDFTIEVERSLRVLDGA
                     VCVLDSSQGVEPQTETVWRQGDKYNVPRIVFANKMDKTGADFYMCVGDIKEKLGARAV
                     PIQIPIGTESSFKGVVDLIRMKAILWEGDGKDAKMIEQDIPADLADKAAEYRAAMIEA
                     AVEMDDDVMAAYLDGTEPDEETLKRCIRKATITRAFYPMLCGSAFKNKGVQPLLDAVV
                     DYLPAPSDREAFKATNPKTGEEVVRRPSDDEPLSMLAFKIIDDPHVGSITFCRVYSGK
                     VQSGMALANTTRDKKERVGRMYLMHANAREQIEEAYAGDIIALAGLKDTRTGETLSDP
                     NKPVLLEKMEFPEPVIEQAIEPKSKADQERMALALQKLAAEDPSFSVSTDPESGQTRI
                     KGMGELHLDIKIDILRRTHKVDVSVGAPQVAYRETLARSAEVDYTHKKQTGGTGQFAR
                     VKLRLEPNEPGKGNVFASEIVGGVVPKEYIPGVEKGVQSVWDSGVLIGFPMVDTKVTL
                     FDGAFHEVDSSVIAFEIAARAAMREGATKAGVKLLEPIMDVEVVTPGDFVGNVIGDIN
                     SRRGQIRSQEMRGNATVIRAYVPLANMFGYVSQLRSMSQGRASYTMQFAHYAEVPRAV
                     AEEVKAKYA"
     gene            11056..>11192
                     /locus_tag="VF226_08310"
     CDS             11056..>11192
                     /locus_tag="VF226_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7074028.1"
                     /translation="MAKQKFERTKPHVNVGTIGHVDHGKTTLTAALTKVSADQGWTTE
                     AV"
BASE COUNT         2235 a         3485 c         3714 g         1758 t
ORIGIN      
        1 ctaccgggtc atgcggcccg gcgagccgcc gacggtcgaa gcggccgaaa cgatgttcca
       61 cggcctgttc ttcgatccgg agcgctacga tctgtcggcc gtcggccgcg tgaagatgaa
      121 catgcgcctc gacctcgacg cgccggacac cgtccgtgtg ctgcgcaagg aggacatcct
      181 tgccgtggtc aagacgctgg tcgacctgcg cgacggcaag ggcgagatcg acgacatcga
      241 ccacctcggc aaccgccggg tgcgttcggt cggcgagctc atggagaacc agtaccgcgt
      301 cggcctcttg cgcatggagc gcgcgatcaa ggagcgcatg tcgtcggtcg atatcgacac
      361 ggtcatgccg caggacctga tcaacgccaa gccggcggcg gcagctgtgc gcgagttctt
      421 cggctcctcg cagctcagcc agttcatgga ccagaccaac ccgctcagcg agatcacgca
      481 caagcggcgt ctgtcggctt tgggaccggg cggtctgacg cgtgagcgcg cgggcttcga
      541 ggtgcgcgac gtgcacccga cgcactacgg ccgcatctgc cccatcgaga cgccggaagg
      601 cccgaacatc ggcctgatca actcgctggc gacgttcgcc cgcgtgaaca agtacggctt
      661 catcgagagc ccgtaccgca aggtcgtgaa cggcaagctc accgacgagg tggtctacct
      721 ctcggccatg gaggagatgc gccaccacgt tgcccaggcg aacgccgagg tggacgagga
      781 aggccggctg gtcggcgacc tcgtcacctg ccgctaccag ggcgacgtca ttctcgtctc
      841 gccggacagg gtcgattaca tcgacgtgtc gccgaagcag ctcgtgtctg tcgctgcggc
      901 gctcattccg ttcctcgaga acgacgacgc caaccgcgcc ctcatgggct cgaacatgca
      961 gcgccaggcg gtgccgctga tcaaggccga ggcgccgctc gtcggcacgg gcatggagcc
     1021 gatcgtggcc cgtgactccg gtgcggccat cgcggcccgg cgtaccggcg tcgtggacca
     1081 ggtggacgcg acgcgcatcg tcatccgcgc gaccgaggag accgacccgt cgaagccggg
     1141 cgtcgacatc taccggctgc gcaagttcca gcgctcgaac cagaacacct gcatcaacca
     1201 gcggccgctc gtgaacgtcg gtgacacggt gcaggcgggc gacatcatcg ccgacggccc
     1261 ctcgacggat ctcggcgatc tggcgctcgg caagaacgtg ctcgtcgcgt tcatgccgtg
     1321 gatgggctac aacttcgagg actcgatcct gctctccgag cgggtcgtct cggaggacgt
     1381 gttcacctcg atccacatcg aggaattcga ggtgatggcc cgcgacacta agctcggtcc
     1441 ggaagagatc acgcgcgaca tcccgaacgt ttcggaagag gcgctcaaga acctcgacga
     1501 ggcgggcatc gtgtacatcg gggccgaggt gaatcccggc gacatcctcg ttggcaagat
     1561 cacgccgaag ggtgagagcc cgatgacgcc ggaggagaag ctgcttcgcg ccatcttcgg
     1621 tgagaaggcc tccgacgtgc gcgacacctc gctcaggctg ccgccgggcg tgtccggcac
     1681 ggtggtcgag gtgcgcgtgt tcaaccggca cggcgtcgag aaggacgagc gcgccatggc
     1741 gatcgagcgc gaggagatcg agcgcctggc caaggaccgc gatgacgagc tgcagatcct
     1801 cgatcgcaac gtctacggcc gcctgaagga cgcgctgctc ggcaaggaag tcgccaaggg
     1861 tccgaagggg gcccgcaaag gcactgaaat cgacgaggcg gtcctggccg acatcccgcg
     1921 cagccaatgg tgggagatcg ggctcaagga cgagaaggct caggccgagg tcgaggccat
     1981 ccagaagcag tacgaagagg ccaagaagag cctcgagcgc cgcttcgtgg acaaggtcga
     2041 caagctgcag cgcggtgacg agctgccgcc aggcgtgatg aagatggtca aggtcttcgt
     2101 cgcggtgaag cggaagatcc aacccggcga caagatggct ggccgccacg gcaacaaggg
     2161 cgtcgtgtcg atgatcgtgc ccgtcgagga catgccgttc ctcgaggacg gcacgcccgt
     2221 cgacgtggtg ctgaaccccc tcggcgtgcc ctcgcgcatg aacgtcgggc agatcctcga
     2281 gacgcatctc ggctgggcct gccgcggact cgggcgcatc atcgatgagg cgctggagca
     2341 gtaccgcgag agcggccagc tggatcgtct caaagagacc attgagaagg tctacgggaa
     2401 ggctgacggc gtcagcaaac tgacgaacga ggagctcgtc gcgttgggcg agcagcttcg
     2461 cgtaggtgtg ccggtggctt cgccagtgtt cgatggtgcc cgcgagacag acatcaacga
     2521 gatgctgcag ctcgccggct tcgacaccac gggccaggtc acgctctatg acgggcgcac
     2581 gggcgagccg ttcgatcgca aggtcacggt cggctacaag tatctgctga agctgcacca
     2641 ccttgtcgac gacaagatcc atgcgcgctc catcggtccc tacagtttgg tcacccagca
     2701 gccgctgggc ggcaaggcgc agtttggtgg ccagcgcttc ggcgagatgg aggtgtgggc
     2761 gctcgaggcc tatggcgccg cgtacaccct gcaggaaatg ctgacggtga agtcggacga
     2821 cgtggccggt cgcaccaagg tctacgaggc gatcgtccgt ggcgacgaca ccttcgaggc
     2881 cggcattccc gagagcttca acgtgctcgt gaaggaaatg cgggcgctgg gcctcaacgt
     2941 cgacctgatc aactcggaag caccggcgct cgaggaggca gaggaagagc agccggtgcg
     3001 gccgcaactg ccgtcgccag ccgcagagtg agacgaaacc cgctccccgt cgcgaaagcg
     3061 ccggggagca gcccggagcg ggggaggatg agcggaagaa cgcgatctcc ctcgcccgac
     3121 tctcttcccg agtggggcac gggcttgaga gacaatcgat cgattttacc tggagcgcgc
     3181 tgcagcaccg cgcgcgagga gagagccgaa tgaacgagat ctcgaatatt ttcaaacagc
     3241 agcaggtcct gccgactttc gaccagatca agatctctat cgccagcccc gaaaccatcc
     3301 tgtcgtggtc gttcggcgag atcaagaagc ctgagaccat caactaccgc acgttcaagc
     3361 cggagcggga cgggctgttt tgcgcgcgca tcttcggccc gatcaaggac tacgagtgct
     3421 tgtgcggcaa gtacaagcgc atgaagtaca agggcctgat ctgcgagaag tgcggtgtcg
     3481 aggtgacgct cgcaaaggtg cggcgcgagc gcatgggcca tatcgagctc gccgctcccg
     3541 tggctcacat ctggtttttg aagtcgctgc cctcccgcat cggcctgctg ctcgatatgt
     3601 cgctgaagga cctcgagcgg gtactctact tcgagaacta catcgtcatc gagccgggcg
     3661 tttcgacgtt ccgcgaaaag cagcttatca ccgaggaaca gtacaaccag gcgatcgagg
     3721 agttcggccc cgacagcttc acggcgggta tcggcgccga ggcgatccgt cagcttctgg
     3781 cctccctgga cctgcacaag atccgggatg agctgcgcca ggagatccag gagtcgacca
     3841 cggagctgaa gccgaagaag cttgcgaaga ggctcaaggt catcgaggcg ttcatcgagt
     3901 ccggcaaccg gccggagtgg atgatcatga cggtcatccc ggtcatcccg cccgagctgc
     3961 gcccgctggt gccgctcgat ggcggccgct tcgccacgtc cgatctcaac gacctctacc
     4021 gtcgcgtcat caaccgcaac aaccgcctga agcggctgat ggagctgcgc gcgcccgaca
     4081 tcatcatccg caacgagaag cggatgctgc aggaggctgt tgacgcgctg ttcgacaacg
     4141 ggcggcgcgg gcgcgtcatc acgggcgcca acaagcgccc gctgaagtcg ctcgccgaca
     4201 tgctgaaggg caagcagggc cgcttccgcc agaacctgct cggcaagcgc gtcgactatt
     4261 cgggccgttc ggtcatcgtg gtcggcccgg agctgaagct gcatcagtgc ggcctgccga
     4321 agaagatggc gctcgagctg ttcaagccgt tcatctacgc gcggctgcag acgctcggcc
     4381 aggcggcgac cgtcaagcag gcgaagaagc tggtcgagaa agagaagccg gaagtctggg
     4441 acgtgctcga cgaggtgatc cgcgagcatc cggtgctgct caaccgcgca ccgacgctgc
     4501 accgcctcgg catccaggcg ttcgagccgg tgctgatcga gggcaaggcg atccagctgc
     4561 atccgctggt ctgcgcggcc ttcaacgctg acttcgacgg cgaccagatg gccgtgcacg
     4621 tgccgctgtc actcgaggcg cagctcgagg cgcgcgtgct gatgatgtcg accaacaaca
     4681 tcctgcaccc ggccaacggc tcgcccatca tcgtgccgag ccaggatatc gtgcttggcc
     4741 tgtactacct gtccatggtt cgtgacggcg agccgggcga ggggatgatc ctcggatccg
     4801 ccgccgaagc gcgtcatgcg ctcgaggctg gcaccgtgac cctgcatgcc aaggtcaagg
     4861 gccgcttccg ctcgctcgat gaaaacggca acgaggtggt ccaactctac gacaccacgc
     4921 ccggccgcat gatcctgggt gagctgctgc cgcagcaacc gggagtcaag tttgaactgg
     4981 tgaaccagct cctcacgaag aaggccatct ccagcatgat cgatgccgtc taccgcaatt
     5041 gcgggcagaa ggagacggtc atcttctgcg accggatcat gcagctcggc ttctaccatg
     5101 ccttcaaggc gggcatctcg ttcggcaagg acgacatggt cgtaccggac tcgaaggcgc
     5161 ggatcgtcga ggagacgacc gagctcgtgc gcgagttcga gcagcagtac aatgacggcc
     5221 tcatcacgca gctcgagaag tacaacaagg tggtcgacgc gtggtcgaag tgcaccgacc
     5281 agatcgccaa ggagatgatg gatcacatct ccgcggtccg gaaggacagt aacggccgcg
     5341 agctgcccat caactcgatc tacatgatga gccactcggg tgcccgcggc tcgccggcgc
     5401 agatgaagca gctcgccggc atgcgcggcc tgatgaccaa gccgtcgggc gagatcatcg
     5461 agacgccgat catctccaac ttcaaggagg gtctgtcggt tctcgagtac ttcaactcga
     5521 cgcacggcgc ccgcaagggc ctcgcggaca cggccttgaa gaccgccaac tccggctacc
     5581 tgacgcgccg tctcgtggac gtcgcgcagg atagcatcat cgtcgaggag gactgcggga
     5641 ccgacgccgg catcaacgtg caggcggtgg tcgacgcagg ccaggtggtg gtctcgctgg
     5701 gcaaccgtgt cctgggccgt acggcggctg aggacatcat cgatccagcg accggcgagg
     5761 tcatcgtccc cacaggcaag ctgatcgagg agcgcgaggc ggaggcgatc gagagagccc
     5821 agatccagga ggttcgcatc cgttcggtgc tgacctgcga gacggtgacg ggcgtctgcg
     5881 ccaagtgcta cggccgcgat ctggcacgcg gcacgccggt caacatcggt gaggccgtcg
     5941 gcgtcattgc ggcccagtcg atcggcgagc caggcacgca gctcacgatg cgcaccttcc
     6001 acatcggcgg tacggcgcag gtggtcgata cgtccttcat cgagtcgaac ttcaacggca
     6061 cggtgaggat caagaaccgc aacgtcgtgc gtgacagcca ggggcgcctg atctcgatgg
     6121 gccgctcgat gtcgatctgc atcgtcgacc agtccggcag ggagctggcc acgcacaaga
     6181 tcacctacgg tgcgcgtctg ctcgtggacg agggcgacac ggtcaagcgc ggcacgcgcc
     6241 tggcgcagtg ggacccgtac acgcgaccca tcctcaccga ggtcgacggc actgtcgact
     6301 tcgaggatct ggtcgagggt gcctcgatgc gcgagcagac cgacgaggtc aagggcacga
     6361 ccaatcgcgt cgtcatcgac tggcgtgcct cgccgcgcgg tgccgacctg aagccggcca
     6421 tcgtcatcaa ggacgagaac ggtaagccca ttaaggtggc ccgtggcggc gatgcccgct
     6481 acctgctgcc cgtggacgcg atcctgtcgg tcgagccggg tgcacaggtc aaggcaggtg
     6541 acgtgctcgc tcgtatcccg atggcgtcgg cccgctcggg cgacatcacc ggtggtctgc
     6601 cgcgcgttgc ggagctgttc gaagcccgcc gtccgaagga tcatgcgatc atcgcggaaa
     6661 tctccggcac ggtcgagttc ggcaaggact acaagaacaa gcagcggatc acgatccgtc
     6721 cggacgacga ggagcagccg ccggtcgagt acctgatccc gcgtggcaag agcctcgccg
     6781 tgcagcatgg cgaccgtatc gaacggggcg acttcgtcta cgacggccac ccggcaccgc
     6841 acgacatcct ggcgatcaag ggcgtcgagg aactggcgaa ctacctgatc aacgagatcc
     6901 aggacgtcta ccggctgcag ggcgtgacga tcaacgacaa gcacatcgag gtaatcgttc
     6961 gccagatgct gcagaaggtc gaggtgacgg atcccggcga cacgacgttc atcaagggcg
     7021 agcagatcga ccgcatcgag ctcgacgagg tgaatgccaa gatcgagaag cagggcgggc
     7081 ggccggcaac ggcgcagccc atcctgctcg gcatcaccaa ggcgtcgctg cagactcgct
     7141 cgttcatctc tgcggcctcc ttccaggaga ccacgcgcgt gctcaccgag gccgccgtca
     7201 acggcaaggt cgacacgctc gagggcctga aggagaacgt gatcgtcggc cggctcatcc
     7261 cggccggcac gggcggcatg ctgcgccgcc tgcgccggat cgcggccaag cgtgacgagc
     7321 tcatcgcccg cgagcgggcg aaggccgact ccgagcgggc tctggctggc ccggacctgg
     7381 agatgcctcg ggaacggcgg cgccgccgcg ccgaggaggc ggccgagtag tcgggtcacc
     7441 ggccgtgttg aaacggaaat ggccacccac gggtggccat tttcatttgg tggtgctcac
     7501 ggtcgcctcg atcgcccgat cttcccttcg gcagtcggcg cacccatatt taccttgcgt
     7561 cggcttactg cacgcgcaat cgcggtcgga agtagggtgg tgcggcggaa aaaactcatc
     7621 tggcgcgcct cattgctgtt gacctgaggg gggcgcctgt atatggtccg cgcactctca
     7681 cggtaggtgg taggccgcat cgggcgctgg cgaatgcttc gggcctgaat cctaaacgct
     7741 accgtagcaa agcagagaac aacttcggcc catgatcccc ggactcgtgc cgcggatcct
     7801 ctggtttcgt cgtacccgcc gcgctccggc atggaagtgg caggtgcggc ttggcgcttc
     7861 ccgcgtcccg tgatgcgggg agctctcgtg gaatggaaga aggcgcatgc cgacgataca
     7921 gcagctggtc cggaagccgc gacagcagaa ggtttaccgc gagaaatcgc gccacatgga
     7981 gggctccccg cagaagcgcg gggtctgcac gcgcgtctat acgacgacgc cgaagaagcc
     8041 gaactcagcc cttcgcaagg tcgccaagat ccggctgacc aacggctacg aggtgatcgg
     8101 ctacatcccg ggcgagggcc acaacctgca ggagcactcc gtggtgctga tccgcggcgg
     8161 tcgcgtcaag gacttgccgg gtgtccgcta ccacatcatc cgtggcgtcc tcgacaccca
     8221 gggcgtggcc gaccgcaagc agcgtcgctc caagtacggt gcgaagcggc cgaagtgatc
     8281 tgaaacggaa acgatacgtt cattcctgcg ggcgcaggcc atgaggcccc atacgggatg
     8341 ttcgaggtgg aagtgagccg cccagcggct tgagcgaaga caggaacgag acgcatgtcc
     8401 cgccgccaca gagccgagaa gcgcgagatc aaccctgatc ccaagtacgg cgatcaggtg
     8461 atcacgaagt tcatgaatgc ggtcatgaac caaggcaaga agtccgttgc cgagcgcatc
     8521 gtctatggcg ctctcgagcg gatggagagc cgcgccaagc aggacccggt tcaattgttc
     8581 cgtcaggcgc tcgagaacgt gatgcctgca gtagaggtgc gctcccgccg cgtcggtggc
     8641 gcaacctatc aggtgccggt agaggtccgc gctgaccgcc ggcaggcgct tgccatccgc
     8701 tggatcatca cagcggcgcg ggcgcgtaac gagaatacga tggtggagcg gctctcgggc
     8761 gagttgctcg atgccgccaa caaccgtgga acagcggtga agaagcggga agacacgcac
     8821 aagatggccg aggccaaccg cgccttttca cattaccgct ggtgacgaat tctggagact
     8881 gagcccatgc cgcgctcaca caagatcgag gactaccgca attttggcat catggcgcac
     8941 atcgatgccg gaaagacgac gacgaccgag cggatcctct atttcacagg caagtcgtac
     9001 aagatcggcg agacccacga gggctccgcg accatggact tcatggagca ggagcaggag
     9061 cgcggcatca ccatcgcttc ggctgcgacg acctgcttct ggaagggtaa gcggctgaac
     9121 atcatcgaca ccccaggcca cgtcgacttc acgatcgagg tggagcgcag cctgcgtgtg
     9181 ctcgacggtg cggtatgcgt gctcgactcg agccagggtg tggagccgca gacggaaacg
     9241 gtctggcgcc agggcgacaa gtacaacgtt ccccgcatcg ttttcgcgaa caaaatggat
     9301 aagaccggcg ccgacttcta catgtgcgtc ggtgacatta aagagaagct cggtgcgcga
     9361 gctgtaccca tccaaatccc catcggaact gaaagcagtt tcaagggtgt cgttgacctc
     9421 atccggatga aggcgatcct ctgggagggt gacggcaagg acgcgaagat gatcgagcag
     9481 gacattcctg ccgatctggc tgacaaagcg gcggagtacc gcgccgccat gatcgaagcg
     9541 gccgtcgaga tggacgacga cgtcatggcg gcctacctcg acggtaccga gcccgacgag
     9601 gaaacactca aacgctgcat ccgtaaggcg accatcacgc gcgcattcta ccccatgctg
     9661 tgcggctcgg cgttcaagaa caagggcgtg cagccgctgc tcgacgcggt cgtcgattac
     9721 ctccctgctc catccgatcg cgaggccttc aaggcgacga atccgaaaac gggtgaggaa
     9781 gtggtccgcc gtccgtcgga cgacgagccg ctgtcgatgc tggccttcaa gatcatcgac
     9841 gatccgcacg tcggctcgat tacgttctgc cgtgtctact cgggcaaggt gcagagcggc
     9901 atggcgctcg ccaacaccac gcgcgacaag aaggagcgcg tcgggcgcat gtacctgatg
     9961 cacgccaacg cccgcgagca gatcgaggag gcgtacgccg gggacatcat cgctctggcc
    10021 ggcctgaagg acacccgcac gggggagacg ctgtccgatc ccaacaagcc ggtactgctg
    10081 gagaagatgg agttccccga gcccgtcatc gagcaggcga tcgagccgaa gtcgaaggcg
    10141 gatcaggaga gaatggcgct ggcgctgcag aagctggcgg ccgaggaccc ctccttcagc
    10201 gtgtcgaccg atccggagag tggccagacc cgcatcaagg gcatgggcga gctgcacctc
    10261 gacatcaaga tcgacatcct gcgccggacg cataaggtcg acgtcagcgt cggggcgccg
    10321 caggttgcct accgcgagac gttggcccgg tcggccgagg tcgactacac gcacaagaag
    10381 cagaccggcg gtaccggtca gttcgcccgc gtgaagttgc gtctcgagcc gaatgagcct
    10441 ggcaagggca acgtattcgc ctctgaaatc gtcggtggcg tcgtgcccaa ggagtatatt
    10501 ccgggcgtcg agaagggtgt tcagtcggtg tgggattccg gtgttctgat cgggttcccc
    10561 atggtcgaca ccaaggtgac gctgttcgac ggcgctttcc acgaggtgga cagctccgtg
    10621 atcgcgttcg agattgccgc ccgggctgcc atgcgtgaag gtgcgaccaa ggcaggcgtc
    10681 aagctccttg agccgatcat ggacgtcgag gtggtgacgc ctggcgactt cgtcggcaac
    10741 gtcattggcg acatcaacag ccgccgtggt cagatccgca gccaggagat gcggggcaac
    10801 gccaccgtca tccgggccta cgtgccgctc gccaacatgt tcggctacgt cagccagctg
    10861 cgctcgatga gccagggccg cgcgtcctac accatgcaat tcgcgcacta cgccgaggtg
    10921 ccgcgcgcgg tcgcggaaga ggtgaaggcg aagtacgcct gatccccgcg gggaaaacag
    10981 aacagctgga tcgaggcttc ggccgtgatc cgtcgaagga cagaactgga ttaaatgaac
    11041 tgaggagagc caccgatggc caaacaaaaa tttgagcgga cgaagccgca cgtgaacgtg
    11101 ggcacgatcg gtcacgttga ccatgggaag acgacgctga cggcggctct gacgaaggtc
    11161 tcagcggatc agggctggac gacggaggcg gt
//
LOCUS       DASVDU010000131         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1172440, whole genome shotgun sequence.
ACCESSION   DASVDU010000131 DASVDU010000000
VERSION     DASVDU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172440"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VF226_08315"
     CDS             complement(<1..1652)
                     /locus_tag="VF226_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX7074029.1"
                     /translation="MLHSVGTQEVIPLAEFITDFGDGLLDAVREQNPPLFDGDVNLAR
                     EAAMDRLLRKPFPAQRAVVQAVSRLLIDEDQPAGVINAEMGTGKTMMAIAAAAIMHEA
                     GYPRTIVIAPPHLVYKWRREIRETVPGATVWVLNGPDTLRKLLQLRQMGKPPAGPEFF
                     VLGRVRMRMGFNWRPTFTSREVIVANELGRQKLRYATCSDCGAWVTGPVSGTEGDAPL
                     TIERARIQLASDRQACKACEAPLWSLVHPHSGSRSRRELVLDAMRRIPTVGSKTAERL
                     VTAFGEDMLAGMLEDNLYEFINLMNEDGELFFHDRQAQRLERAMANMEFSFGQGGYQP
                     TEFIKRYLPQGFFGLLVVDEGHEYKNEGSAQGQAMAVLARKCRKVLLLTGTLMGGYAD
                     DLFYLLWRLNPRMMIEDGYRYNARRSLGAAAMAFMREHGVIKDIYKESESTSHRTARG
                     RNISHRAAKGPGFGPKGIMRYVLPLTVFLKLKEIGGDVLPPYAEHFEEVGMTEAQAEA
                     YRKLCDTLKQELRTALVKGDKSLLGVVLNVLLAWPDCAFRDEL"
     gene            complement(1764..2027)
                     /locus_tag="VF226_08320"
     CDS             complement(1764..2027)
                     /locus_tag="VF226_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074030.1"
                     /translation="MLVGSGTVSTPPRIVSATISPMPQEVWDPLPIVTATFSDGSVKK
                     LFSYFPDEISFLPTEFVGLTEEEAKTLKAKKDSVYLTGGYRPR"
     gene            complement(2110..2742)
                     /locus_tag="VF226_08325"
     CDS             complement(2110..2742)
                     /locus_tag="VF226_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074031.1"
                     /translation="MFRIKEVADVFVDACVRDEEGRLLFLSCFGRDTAIQQLFAAFYL
                     KPTEGGLEVFHLAEPGAGAHAAGETVRLGSAEGLQKLAGRLPRENVFGNLAHTWIYDP
                     AIVRPDRATRTAWVLDPQHSAESGADKVLSRVWEVFKLLSPVPLLDEWQTTLMKACFD
                     DCVKAMADSAFPPLGRVSAQKVTVPDSFLGVVSTLVKRGELALSKLKQAA"
     gene            complement(2773..>3145)
                     /gene="radC"
                     /locus_tag="VF226_08330"
     CDS             complement(2773..>3145)
                     /gene="radC"
                     /locus_tag="VF226_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX7074032.1"
                     /translation="ALTSPQAVRDYLRISLMGRSYEVFMVLFLDAQHRLIEAEEMFRG
                     TLTQTSVYPREVVKRSLYFNAAAVIFAHNHPSGVAQPSQSDELLTRSLREALSLVEVR
                     VLDHFVIGGNAVMSFAERGLL"
BASE COUNT          575 a         1038 c          967 g          565 t
ORIGIN      
        1 agctcgtcgc gaaacgcgca gtccggccag gcgagcaaca cgttaagcac cacgccgagc
       61 aggctcttgt cgcccttgac gagggccgtg cgaagctcct gcttcaacgt atcgcacagc
      121 tttcggtacg cctcggcctg agcctccgtc atcccgactt cctcgaagtg ctcggcgtac
      181 ggcggcagga catcgccgcc gatttccttc agcttcaaaa agaccgtcaa gggcagcacg
      241 taacgcatga ttcccttcgg accgaatccc ggacccttgg ccgcgcggtg gctgatgttg
      301 cgtccgcggg cggtacggtg cgaggtgctc tcggattcct tgtagatgtc cttgatcacc
      361 ccgtgctccc tcatgaaggc catggcggcc gcgccgaggc tccggcgggc gttatagcgg
      421 tagccgtcct cgatcatcat gcgcgggttc aaccgccaca ggagatagaa gaggtcgtcc
      481 gcgtagccgc ccatcagcgt gccggtgagc agcagcacct tccggcattt cctggcgaga
      541 actgccatcg cctggccctg ggccgaacct tcgttcttgt actcgtgccc ctcgtccacc
      601 actaggaggc cgaagaagcc ttgcggcagg tagcgcttga tgaactcggt cggctggtat
      661 ccgccctgcc cgaacgagaa ctccatgttg gccatggcgc gctcgagccg ctgcgcctgc
      721 cggtcgtgga agaagagctc cccatcctcg ttcatcagat tgatgaactc gtagagattg
      781 tcctcgagca tcccggcgag catgtcttcg ccgaaagccg taacgagccg ctccgcggtc
      841 ttgctaccga cggtagggat gcggcgcatc gcatcgagca ccagttcccg gcgcgaccgg
      901 ctgccggaat gcgggtgaac gagactccac agcggagctt cgcaggcctt gcacgcctga
      961 cgatcgctgg cgagctgaat gcgtgcccgc tcgatcgtca gcggagcatc gccctccgtg
     1021 ccgctcacgg ggccggtgac ccaggccccg caatccgagc acgtggcata gcgcagcttc
     1081 tgccggccaa gctcgttcgc cacgatgact tcgcgtgacg taaacgtcgg ccgccagttg
     1141 aaccccatgc gcatgcgtac gcggcccagg acgaagaact cgggcccggc cgggggtttc
     1201 cccatctgcc gcaactgcag gagcttgcgg agcgtatccg ggccgttcag cacccacacg
     1261 gtcgccccgg ggaccgtctc gcggatctcc cggcgccact tgtagaccag gtgcggcggc
     1321 gcgatgacga tggttcgcgg atagcccgcc tcgtgcatga tcgccgccgc ggcgatcgcc
     1381 atcatggtct tgccggtacc catctcggca ttgatcacgc cggcgggctg gtcctcgtcg
     1441 atcagcagac ggctcacggc ctgcacgacc gcccgctgag ccgggaatgg cttccgaaga
     1501 agccgatcca tggccgcctc gcgggcaaga ttgacgtcgc catcgaagag cggcgggttt
     1561 tgctctcgaa ctgcatcgag cagcccatcg ccgaagtcgg tgatgaactc cgcgagcgga
     1621 atgacttcct gggttcctac actgtgaagc atggttctct cctggatgaa aaagcggaga
     1681 gcaccctccc ctgcgggaag gtgactcccc gcagggttga cgaaaagatg gccgctacgc
     1741 ggcctttgac gtggtccgaa tctctaccgg ggtcggtaac cgccggttag gtagaccgaa
     1801 tcctttttgg ctttcagcgt ctttgcctct tcctcggtca gcccgacaaa ctcggtgggc
     1861 aagaacgaga tctcgtccgg gaagtaactg aagagcttct tcacggagcc atcgctaaac
     1921 gtggctgtaa cgataggcag cgggtcccac acctcctgcg gcatgggact gatcgtggcg
     1981 ctgacgatgc gaggcggcgt gctcacagtc cccgatccta caagcatggt tctctccttg
     2041 tcgaagatcg cggagagcac ctccctgcgg gaaggtgact ccccgcaggg ttgacggaac
     2101 tagcgtccgc tatgccgctt gcttgagctt ggagagcgcg agctccccgc gcttgaccaa
     2161 cgtggatacg acgccgagga acgaatccgg caccgtcact ttctgcgccg agacccggcc
     2221 gagcggcggg aacgccgaat cggccatggc cttgacgcaa tcgtcgaaac acgccttcat
     2281 gagcgtggtt tgccactcgt cgaggagcgg caccggggaa agcagcttga agacttccca
     2341 caccctgctc agcaccttgt cggcaccgct ttctgcgctg tgctgtggat cgagcaccca
     2401 ggctgtacgg gtagcgcggt ccggccgaac gatcgcggga tcgtagatcc acgtgtgcgc
     2461 caggttgccg aacacgttct cccgcggcag acggcccgcc aacttctgca ggccttccgc
     2521 gcttcccagc cgcaccgtct cgccggcggc atgagcaccc gcgcctggtt cagccaggtg
     2581 gaagacctcg agcccgccct ctgtcggctt gagatagaag gcggcaaaca gctgctggat
     2641 ggccgtatcg cggccaaagc acgaaagaaa caacagccgg ccttcctcgt cgcgcacgca
     2701 ggcatcgacg aagacgtcgg cgacctcctt gattctgaac ataggcatct cctctatcaa
     2761 cgtttaagcg atctacagaa gcccgcgctc ggcgaacgac atcacggcgt tgccgccgat
     2821 cacgaagtgg tcgagcactc tgacctcgac gaggctcagg gcctctctca gcgaacgagt
     2881 cagcagctcg tcggactggc tcggctgcgc gactccggac ggatggttgt gcgcgaagat
     2941 caccgccgcg gcattgaagt agaggctgcg cttcaccact tcccggggat aaacgctcgt
     3001 ctgggtgagc gtgccgcgga acatctcctc ggcctcgatc agccggtgct gcgcgtccag
     3061 aaacaggacc atgaacacct cgtaactccg ccccatgagg gagatgcgca ggtaatcgcg
     3121 caccgcctgg ggcgaagtca gggca
//
LOCUS       DASVDU010000132        11752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1371229, whole genome shotgun sequence.
ACCESSION   DASVDU010000132 DASVDU010000000
VERSION     DASVDU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11752
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371229"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..612)
                     /locus_tag="VF226_08335"
                     /pseudo
     CDS             complement(1..612)
                     /locus_tag="VF226_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611470.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            677..1249
                     /locus_tag="VF226_08340"
                     /pseudo
     CDS             677..1249
                     /locus_tag="VF226_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859398.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1295..1963
                     /locus_tag="VF226_08345"
     CDS             1295..1963
                     /locus_tag="VF226_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7074033.1"
                     /translation="MTRKTKPQLPACAAEIVREYGPFADADQIHGVTHDGHRVWAAVG
                     PRLIAFDPASGQLARTLECAGGAGTAFDGTYLYQIAEARIDKIDPATGEVVASIPAPG
                     NGGDSGLAWAEGSLWVGQYRDRKIHRIDPETGAILGTIECNRFVTGVTWIDGDLWHGT
                     WEADESDIRRIDPESGAVLERLEMPPGTGVSGLESDGADLFYCGGGPSGKVRAVRRPK
                     GAKR"
     gene            2816..3673
                     /locus_tag="VF226_08350"
     CDS             2816..3673
                     /locus_tag="VF226_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7074034.1"
                     /translation="MRSQRIDTDGISLYSEAFGDAGDPPVLLIMGAMASGVWWPEDFC
                     RELAGRGRYVVRYDHRDTGRSTSYEPGQAGYTTEDLADDAVRVLDGYGIGQAHLVGMS
                     LGGYLSQIVALKHPGRVLTLTLIASERLASADPSMPSMDPSILEHHAKAAEVDWSDRE
                     AVVEYQVGAWRLLSGSAHPFDEDAIRAMAVADFDRTPDPRTAFNHATLGDAKRWVGRL
                     HEITAPALVIHGTEDPVLPYAHGLALRDALPSATLLTLEGTGHELHRSDWGVILDAIE
                     RHTRPRLKA"
     gene            3834..4319
                     /locus_tag="VF226_08355"
     CDS             3834..4319
                     /locus_tag="VF226_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7074035.1"
                     /translation="MVQPAKNTVCLWYDGGAEEAARFYARTFPDSSVGAVHRAPGDFP
                     SGKKGDVLTVEFTVMGIPCIGLNGGPAFKHSEAFSFQVATTDQAETDRYWNAIVGNGG
                     EESACGWCRDKWGLSWQITPIALTNAVTNPDPAVAKRAFDAMMQMKKIDIAAIEAACR
                     G"
     gene            4479..4844
                     /locus_tag="VF226_08360"
     CDS             4479..4844
                     /locus_tag="VF226_08360"
                     /inference="COORDINATES: protein motif:HMM:NF040338.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6164 family protein"
                     /protein_id="HEX7074036.1"
                     /translation="MPILLFSLRSVPDDEAEEVRQLLASNRIDFYETPAGRWGISSPG
                     IWLHDGYQLPKARSLIETYQKERFASRRQAFESLKQQGKHRTIVHAIVENPLRFVFYL
                     IVIGVVLYLSIVPFFRLGT"
     gene            4907..5569
                     /locus_tag="VF226_08365"
     CDS             4907..5569
                     /locus_tag="VF226_08365"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HEX7074037.1"
                     /translation="MLSADYPIQCRCGRLQGTLSRTARVTRLSCYCLDCQSYAHALGD
                     PGRILDAHGGTEVVATLQQYLTFTAGNESLACLSLSPQGILRWYAGCCNTPIANTTRD
                     PKLSYVGIVHTCLGAPAARTAAFGPPRAPVNTRHAKGKVATSALNVSNSIVRVVARVL
                     RARLDGSWRRSPFFRSGSSTPVVARRVLSVEERERARNAARVWQPEESSQSGGAVASK
                     PD"
     gene            5903..6520
                     /locus_tag="VF226_08370"
     CDS             5903..6520
                     /locus_tag="VF226_08370"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX7074038.1"
                     /translation="MATGWRIEGQYMETCNCDFLCPCITSNLTARPSEGDCKAAVAMR
                     IDKGQKDGVSLDGLSFIVLMHAPGAMAEGNMTVGLIVDERASDEQVEAISAIATGAAG
                     GPMAPLGPLVGKVAGVEKRPIRFEADGMKFSVRAGELIDQAIEGLPGPARPGEPIYID
                     NTAHPVNSKLGLAKATRSKFDAFGIQWDDTSGTRNGHFAPFAWAG"
     gene            6508..7314
                     /locus_tag="VF226_08375"
     CDS             6508..7314
                     /locus_tag="VF226_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX7074039.1"
                     /translation="MGGVERPTPLHRGIEAALRRDRLVVAAGLAGVVALAWLYLWRYA
                     ASMPAGNGAAMEMTMPSMSAMGPAAFALTFVMWVVMMAGMMLPSAAPTILFYGALVRK
                     NAARGTVLPAVWVFVSGYLLVWTGFSLAAVLLQTALGRAALLTPAMTSASNRLSGMLL
                     VAAGVYQLTPLKDLCLSKCRNPVQFFLTHWRPGAGGALQMGITQGAYCLGCCWLLMLL
                     LFVVGVMNLAWVALIAAFVFLEKLLPAGRLSSRLAGVALIVAGSAVLVAA"
     gene            7609..8508
                     /locus_tag="VF226_08380"
     CDS             7609..8508
                     /locus_tag="VF226_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7074040.1"
                     /translation="MIDDTWLAEKFEAHRARLKSVAHRILGSEAEAEDMVQETWLRLS
                     RAGAEEVENLGAWLTTVVARAALDRLRARRARHEESLEEDSIEAQPLERDSPEDEALL
                     ADSIGPALLVVLDRLAPAERVAFVLHDMFGLSFEEIAPIVERTPTAARKLASRARQRV
                     RGPQDAPKAALGLQRKVVSAFLAASRAGDFGALLELLDPNVVLRADTFAREKSAARRA
                     QGAPLLEPETRGAAKVAETFKGRARAAHLALLGGAVGAAVVPGGELRVVFVFAMEHGK
                     ITAIDIVGDPARLAALGVRLLQD"
     gene            8582..8959
                     /locus_tag="VF226_08385"
     CDS             8582..8959
                     /locus_tag="VF226_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7074041.1"
                     /translation="MSSVSVRYIIDDVPAAMHFYTTLLGFTVEHDASPAFASVVRDGV
                     RLLLSGDGSSGKRPLPDGRQQVPGGWNRVHLEVTDLPAEVERLRAAAVSFRTPDLVGG
                     PGGAQVILDDPSGNPVELFQRRR"
     gene            9163..9546
                     /locus_tag="VF226_08390"
     CDS             9163..9546
                     /locus_tag="VF226_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7074042.1"
                     /translation="MTARQTVETLKAITEAFNAHDLDAIMAFFAEDCSLDMPRGPEPW
                     GQRCAGKAAVREGLATRFKGLPDVHYSDDRHWVSGNKGVSEWLLTGTTPEGVPVRVRG
                     CDHWEFRDGKVIRKDSYWKIVEKRA"
     gene            9739..10140
                     /gene="mscL"
                     /locus_tag="VF226_08395"
     CDS             9739..10140
                     /gene="mscL"
                     /locus_tag="VF226_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX7074043.1"
                     /translation="MLKGFRDFLLRGNVVDLAVAVVMGVAFTALVNSIVTNLITPIIA
                     LAGGAPDFSAIRTGPILWGNILNDVLTFVITAAAVYFALVMPINALMARQRAPAPSAA
                     STRKCPECLSEIPIGARRCAYCSVQISRAAG"
     gene            10304..10861
                     /locus_tag="VF226_08400"
     CDS             10304..10861
                     /locus_tag="VF226_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7074044.1"
                     /translation="MRKLIMWNLITLDGYFEGEKSWDLPWHERVWGEELERFSLDQLQ
                     SADMLVFGRVTYEGMAAYWQTATGAIADYMNSLPKVVCSRTLQSAGWNNSTLVRQDAA
                     KELAKLKQQGNGDMFVFGSANLSETFMNENLFDEYRIGIAPVVHGRGRLLFSRGSRPQ
                     WLQLLEARPLSNGCLILRYKSEAAA"
     gene            10960..11520
                     /locus_tag="VF226_08405"
     CDS             10960..11520
                     /locus_tag="VF226_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7074045.1"
                     /translation="MRPLRYSINVTLDGCYDHRAMIPDEELHRHAAEGIAQADALLFG
                     RVIYEMMESAWREPAETGVLPDWMEPWMVPFARTIDAARKYVVSSTLERVDWNAELVR
                     GDLAHAVRRLKQEPGKGLATGGVTLPQALAELGLIDEYEFIVHPRLASHGPTLFAGLS
                     KHVDLKLVDRLEFRSGAVVLRYVPKG"
     gene            complement(11643..>11752)
                     /locus_tag="VF226_08410"
     CDS             complement(11643..>11752)
                     /locus_tag="VF226_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEX7074046.1"
                     /translation="RRAIFDYIELFYNRSRLHQTLDYVAPVAYEERAVP"
BASE COUNT         2023 a         3787 c         3878 g         2064 t
ORIGIN      
        1 cgttgggcga ggcgggttgc ggcaggggtt tgcgctcgag gggcccgatg cgctttctct
       61 tgcgccggcg cacggcgagg ccagcctccc ggtagaggcg ataggtcttt ttcacgttta
      121 cgctccagcc ctcgcgcctg agcagcacat ggatgcgccg gtagccatag cggcgcttgg
      181 cggcggcgag cgcgccgatg cgatcgcgaa ggccggtaca ggccggccgg cgcgaacgat
      241 agcggtagag ggagcgggag atcgccatca gcccgcaggc gcgcgtcacg ccgaagcggt
      301 gctcgtccat gagcaccgcg acggcctcgc gcttcgcctg cgggcttacc attttcgctt
      361 gagcagatcc ttcaaggcca ccttgtcgag ctcagcgtcg gccagcaggc gcttgagcct
      421 cgcgttttcc tgctcgaggt ccttcaggcg ccgggcctcg gagaccgtga ggcccgcgta
      481 cttggccttc cagttgtaga aggtggcctc ggagatgccg tgcttgcgac acaggtccga
      541 cgtcttggtc ccggcctcgg cctccttcag caccgcgatg atttgctctt cggtgaaacg
      601 cttcttcttc aatggtccgc tcctccctcg gggcggactc tacctcaggt cgggactaaa
      661 atcggggggc gggtcagaag ggctcctcgc ctcgggcgcg ctggtcgtcg acgcctgccg
      721 tcgcggcctg cgcgcaggca gcgactggcg gccgcttgcg cgccgcccgg tgctgttcgc
      781 gttggcacgc gcgctcgccg aggcgtggcc cggcgacgtc gatcgaaaca cattgatcgc
      841 tcgcgcgttt cgcacccgtc accccaacga gtcgcaccgc gcgcgcctgc gcgtcgagat
      901 cggccgtttg cgcgcactcg tcgcaccgct ggcgcatatc gaggccagcg cgcgcggttt
      961 cgcgctcacg ccgcggggcc gccgtgccgt cgtcgttctg gtgccgccca tcgacggcga
     1021 tcaggcgtcg ctcgcggcgc tgctcgccga cggcgccgcc tggtccacct ccgccctcgc
     1081 gctggccctc ggaaccagcc agcgtacggt ccagcgtgcg ctcctcgagc tggaagccgc
     1141 cggacgcgtg cgctcgatcg ggcgcgcacg cacgaagcgc tggctgtcgc cgccgctggc
     1201 cggattcacg acgatcttgt tactccctgc cgcgctgccg ctcgggtaga ttgctctcgc
     1261 ggcgcattcg aagcgccaca caaggagtag agcaatgacc cgcaagacga aaccgcagct
     1321 accggcgtgc gcggccgaga tcgtgcgtga gtacggcccg ttcgccgacg ccgaccagat
     1381 ccacggcgtc actcacgacg gccaccgcgt ctgggcagcc gtggggccca ggctgatcgc
     1441 cttcgatcct gcgagcggcc agctggcgcg cacgctggag tgcgccggcg gcgccggcac
     1501 cgcattcgac ggcacatacc tctatcaaat cgccgaagcg cgcatcgaca agatcgatcc
     1561 cgccaccggc gaagtcgtgg cgtcgatccc cgcgcccggc aacggcggcg actcgggcct
     1621 ggcctgggcc gagggcagcc tgtgggtggg gcagtaccgc gaccgcaaga tccaccggat
     1681 cgatcccgag accggcgcca tcctcggcac catcgagtgc aaccgcttcg tcaccggcgt
     1741 gacctggatc gacggcgacc tgtggcacgg cacctgggaa gctgatgaga gcgacatccg
     1801 ccgaatcgac ccggagagcg gcgccgtgct cgagcgactg gagatgccgc ccggcaccgg
     1861 cgtcagcggg ctcgagtccg acggggcgga cctgttctat tgcggcggcg ggccgagcgg
     1921 aaaggtccgc gcggtgcgac ggccgaaagg agcgaaacga tgaccaacca caggactgca
     1981 accctgcaga attaaagggg ccgggctcga ggctcgttgc ggctcgggcc aagcccaggc
     2041 gccgtactag tagtggggct gctcgtagac ataggccttg cggtctgcgt gaagcagcta
     2101 taaacatgcc agctaacgtg ccgctggttc cgactcaaca ccagcgaagt gcggttgctc
     2161 gctgcacagc ggcagcgtta gccgtcatcc cgcccatttc gggacacaga ggagactgcc
     2221 atgtccgccg caaagattcg cgaagcactc gcaaaggttg ctgaagtcgt tgcgaaggat
     2281 ccggagaagg cacgtgtcaa gagtgccccc gctaccgccc tgatcgagcg tggcctcaga
     2341 tgtcgggtca cgggtcccgc gggtgagctg atccataccg acatgccgac cggcatgggc
     2401 ggagacgctt cagcgccgaa tcccgcctgg tttatgagag ctgcactggc gtcatgcaat
     2461 gtaaccagaa tcgcaatgcg agccgcagag cggggcatcg accttacgaa gctcgaagtc
     2521 tcggtatcca gcgagaccga cagtcgaggg ttactcgggc ttgatgagaa tgcttccgcg
     2581 gcaatgaggc ccttacacgt gcacgtaaga atcagcgcta ccgacgcctc cgcggaggat
     2641 cttaagaaac tggttgactg ggcatgcgct cattcccccg ttgcgtgcac cgatccgaac
     2701 acggcaacca tggacattca ggtcggatag gctgcggtga tccatcacgg ccaggcgcgg
     2761 tctacggcga atatctcgaa cggatcgagc acagcaactg caccatacgc acatcatgag
     2821 aagccaacga atcgacacgg acggcatctc tctctattcc gaagcattcg gcgacgcagg
     2881 cgatccgcct gttctcttga tcatgggcgc catggcgtcg ggcgtctggt ggccggagga
     2941 cttctgtcgg gagctcgccg gcaggggccg ctacgtggtc cggtacgatc accgcgacac
     3001 cggcagatcg acgagctatg aaccgggtca ggcaggctac accaccgagg atctcgccga
     3061 cgatgctgtc cgcgtgctcg atggctacgg gatcggccaa gctcatttgg tcgggatgtc
     3121 gctcggaggc tatctctcgc aaatcgtcgc gctgaagcat cccggccgcg tgctcacgct
     3181 gacgctcatc gcctcggagc ggctcgcctc cgccgatccg agcatgcctt cgatggatcc
     3241 atcgatcctc gagcaccacg cgaaggccgc cgaggtcgac tggtcggacc gcgaggcggt
     3301 ggtggagtac caggtcggcg cctggcggct cctctccgga tcggcgcacc cgttcgacga
     3361 ggacgcgatt cgcgcgatgg ccgtcgccga tttcgaccgt acgccggatc cgcgcacggc
     3421 ctttaaccat gccactctcg gcgatgcgaa gcgctgggtc ggccgcctcc acgagatcac
     3481 cgccccggcc ctcgtcatcc atggcaccga agacccggtg ctcccctacg cgcacggact
     3541 cgctctgcgc gatgcgctgc ctagcgcgac gctgttgacc ctcgaaggca cggggcacga
     3601 gctgcatcga tccgattggg gcgtgattct cgatgccatc gagcggcata cccgaccacg
     3661 attgaaagcc tagctcaggc tttcgagcga gagcatgctt gatcctttct cgaaaaacgt
     3721 ggtctgtccc ctattgttgt tgctattgtt gttgctttct cgaaaaacgt ggtctgtccc
     3781 ctattgttgc tattgttgct gcagcttcgg ctcatttaca aaggagaagg gacatggtcc
     3841 agccagcaaa gaacacggtc tgtctctggt acgatggcgg cgccgaggag gctgcgcggt
     3901 tctacgccag gacctttccc gattcctccg tcggggcggt gcaccgcgca ccgggagact
     3961 tcccgtcggg aaagaaaggc gacgtgttga ccgtcgaatt caccgtgatg gggattccgt
     4021 gcatcgggct caacggcggg cccgcgttca agcacagcga agcattctcg tttcaggtcg
     4081 caaccaccga ccaggcggaa acggatcgct actggaacgc gatcgtcggc aacggcggcg
     4141 aggagagcgc gtgcggctgg tgccgggaca aatggggatt gtcctggcag atcacgccca
     4201 tcgccctgac gaacgcggtc accaatcccg accctgccgt cgccaagcgc gcgtttgatg
     4261 cgatgatgca gatgaaaaag atcgacatcg cggcaatcga ggcggcgtgc cgcggttgag
     4321 gaacgcggcg gcacgtccgg catggccggt cgaccgcggg taactggggc aggcgtgatc
     4381 gattgctcga accgctcgct cgcaagcgag ggacgaaagg aaggatgcga ctgtggtgac
     4441 gagccggatg ggcctgcgat gaaatagagg cggaacaaat gccaatcctg ctgttctcgc
     4501 tcagaagcgt acccgatgac gaagcggaag aggttcgaca gttgttggcg tcgaaccgaa
     4561 tcgatttcta tgaaacgcct gcgggcaggt gggggatatc gtcgccgggg atctggctgc
     4621 acgacgggta ccaactgccg aaagccaggt cgttgatcga gacgtatcag aaagagcgat
     4681 ttgcgtcgcg ccggcaagcg ttcgaatcgc tgaagcagca gggcaagcac aggacgatcg
     4741 tccacgctat cgtagagaac ccgctccggt ttgtttttta tctcatcgtc atcggtgtcg
     4801 tcctctatct gtctatcgtg ccgttttttc ggttggggac ttgaaggaac cgaggcggaa
     4861 catcaggcag gatgaaacag attctgccca acgagtgtgc cttaccatgc tctccgcgga
     4921 ttatccgatc caatgccgat gcgggcgact gcaaggtacg ctctcgcgca ccgcgcgcgt
     4981 aacccggctg agctgctact gcctggactg tcagagctac gcgcatgcct tgggcgatcc
     5041 cggacgaatt ctcgacgcgc acggcgggac cgaggtcgtc gcaaccctgc agcagtatct
     5101 gaccttcacc gccggcaacg aatcgctcgc ctgtctttcg ctcagcccgc agggcatcct
     5161 ccgttggtac gcaggttgct gcaacacgcc gattgcgaac accactcgcg atccgaaact
     5221 ttcctacgtt ggcattgtgc acacgtgcct cggcgccccg gccgcgcgca cggcagcctt
     5281 cggaccgccg cgcgcgccgg taaacaccag gcacgcgaaa ggcaaggtgg cgacgagtgc
     5341 attgaacgtg tcgaattcca tcgtgcgcgt agttgcgaga gtgcttcgcg cccggctcga
     5401 tggaagctgg cggcgcagcc cgtttttccg gtcgggcagc tccacgcctg tcgtcgcccg
     5461 tcgggtgttg agcgtcgagg aacgggagcg ggcacgaaac gccgcccggg tgtggcagcc
     5521 ggaggaatcg agccaaagcg gcggagctgt agcgtcgaag ccggattgag gcccgttacc
     5581 tcatccgacg cccgtcggct ccgagtgcgc gccgccgcga gacgaaccct gcgaggcctc
     5641 gcgccgctga tcgtcgacgc gatccttcaa gaacggaagc ggcctcgagc cggcccacac
     5701 cgttccgatt tccaaccaga acttgcgctt aagcacgagc tcgccgatcg cttttcctca
     5761 tcaagcgtgc ggaattccgc tgccacggca cgcccgccga cctgtccgcc gacccggaat
     5821 cgcccgagga gtaagtaaag gactaataga gttgtccacg ggaccggagt agtctgccca
     5881 ccttccttcc aggaggacgt ccatggccac cggctggcga atcgaagggc agtacatgga
     5941 gacctgcaac tgcgacttcc tttgcccgtg catcacttcg aacctcacgg cgcgacctag
     6001 cgagggcgat tgcaaggcag ccgtggcgat gcgcatcgac aagggccaga aagacggcgt
     6061 gagcctcgac ggcctgtcgt tcatcgtcct catgcatgcc cccggggcga tggcagaggg
     6121 caatatgacg gtcggtctta ttgtcgatga acgcgcatcc gacgagcagg tagaggcgat
     6181 cagtgcgatt gcgacgggcg cggcaggtgg cccgatggcc ccgctcgggc cgctggtggg
     6241 caaagtcgcc ggcgtggaga agcggccgat tcgcttcgag gcggatggca tgaagttttc
     6301 ggtgcgggcg ggcgagctca tcgaccaggc catcgagggt ctgcccggac ccgcacgccc
     6361 gggcgaaccg atttatatcg acaataccgc ccatcccgtg aacagcaaac tcggattagc
     6421 gaaggccacg cgcagcaagt tcgacgcatt cggcatccag tgggatgaca cgagcggcac
     6481 gcgcaacggc cacttcgcgc ccttcgcgtg ggcggggtag agcgccccac accgcttcac
     6541 cgcggcatcg aggcggcgct gcgtcgcgac cgcctcgttg tcgcggctgg tctcgcgggc
     6601 gtcgttgccc tcgcctggct ctacctgtgg cgctacgccg cgtccatgcc ggccggcaat
     6661 ggcgctgcca tggaaatgac gatgccttcc atgtcggcga tgggcccggc cgccttcgcc
     6721 ctgactttcg tcatgtgggt cgtcatgatg gccggcatga tgctgccgag cgccgcgcca
     6781 acgatcctct tctacggcgc cctggtgcgc aagaacgccg cgcggggcac ggtacttccc
     6841 gcggtgtggg tcttcgtcag cggttatctc ctcgtctgga ccggtttcag cctcgcggcc
     6901 gtgctgttgc agaccgccct ggggcgtgcg gccctgctga cgccggccat gacctccgcg
     6961 agcaaccggt tgagcggcat gctcctggtt gcggccggcg tctaccagtt gacgcccctc
     7021 aaggatctct gcctcagcaa atgccgcaat cccgtgcagt tcttcctgac ccattggcgc
     7081 cccggggccg ggggcgcgct ccagatgggc atcacccagg gcgcgtactg cctcggctgc
     7141 tgctggctgc tgatgctgct gctcttcgtc gtcggcgtca tgaacctcgc ctgggtggcg
     7201 ctcatcgcgg cgttcgtgtt cctggagaag ctgctgcctg ccggccgtct cagctcgcgc
     7261 ctggccggcg tcgccttgat cgtcgctgga tccgctgttc tcgtcgctgc ctgatcggtc
     7321 accgcgctga gcatcaccgc gctcattcag cgagcaggtc gccaaaccgt ttcaggcaga
     7381 gcggccagcc gccctcggga ccggactgct tcgcgcaccc cccgccctcg ccggtcgagg
     7441 cggcgtcccc ggtcaccgac gttgatgtcc gcggcgcggt aacggcttcg ggggcacgac
     7501 gccgggccca ttcgtgcctt caattgcaat ggcgttcgcc gtcctgtcac atttgccgag
     7561 gctggatcgt catgataatg acgggcgacg cacggggaaa cgtgaccgat gatcgatgac
     7621 acctggctgg cggagaagtt cgaggctcac cgggcccgcc tgaagtccgt ggcccaccgg
     7681 atcctgggct cggaggccga ggccgaggac atggtccagg agacgtggct caggctcagt
     7741 cgggccggcg ccgaggaggt cgagaacctg ggcgcatggc tcaccacggt cgtcgcgcgc
     7801 gcggcgctcg accggttgcg cgcccgcagg gcgcggcacg aggagtcact cgaggaggac
     7861 tccatcgaag cgcagccgct cgagcgcgac agccccgagg acgaggcctt gctcgccgac
     7921 tccatcggcc ccgccctgct cgtcgtgctg gaccggctgg cgcccgcgga gcgggtcgcc
     7981 tttgtgctgc acgacatgtt cggcctgtcc ttcgaggaga tcgccccgat cgtggagcgt
     8041 acgccgaccg cggcccgcaa gctcgccagc cgggcgcgcc agcgggtgcg gggaccgcag
     8101 gacgcgccga aagccgcgct cggcctgcag cgaaaggtcg tcagcgcttt cctcgccgcg
     8161 tcccgcgccg gcgacttcgg cgccctgctc gagctgctcg acccgaacgt ggtgctgcgc
     8221 gccgacacct tcgccaggga aaagagcgcc gcgcgccggg cgcagggcgc gccgctgctc
     8281 gagcccgaga cccgcggcgc ggcgaaagtg gccgagacgt tcaagggccg cgcacgcgcg
     8341 gcccaccttg cgttgctggg cggcgcggtc ggcgcggcgg tggtgcccgg cggcgagctg
     8401 cgggtggtgt tcgtctttgc catggagcac gggaagatta cggcgattga catcgtcggg
     8461 gatccggcgc ggcttgcggc actcggcgtt cgactgctgc aggattgagc gcggccctct
     8521 tcgatgttct cggtgggttc tgttcgaggt tggttgtgtt caatcgaatg gaggaaaagc
     8581 gatgtccagc gtcagcgtcc gctacatcat tgacgacgtc ccggcggcga tgcatttcta
     8641 taccacactc ctcgggttca ccgtggagca cgatgcctcg cctgccttcg cctcggtcgt
     8701 gcgcgacggc gtgcgcctcc tcctcagcgg ggacggcagc tcggggaagc gtccgttacc
     8761 cgacggtcgc cagcaggtac cggggggctg gaaccgcgtg cacctggaag tgaccgatct
     8821 ccccgccgaa gtcgagcggc tgcgtgcggc ggcggtctcc tttcgtaccc cggacctcgt
     8881 gggcgggccg ggcggcgccc aggtcattct cgacgacccg tcgggcaacc cggtggagct
     8941 gttccagcgt cggcgatgaa cctccaccga ggggcctgcg aacggcgccg cggggcgaga
     9001 gaattaaagg gaccgggctc gatctaatgt aatgccgtcc gctgcgggaa tgcgtgccca
     9061 gcccctttcg cattgccggg acaggcagga atcggaggcg ccaggccagg aatcgccgct
     9121 ccccgtgcct cggtcgtcag cgcaccgaac agaggaaccc ccatgactgc acggcagacc
     9181 gtcgagacct tgaaggcgat caccgaggca ttcaacgcgc acgacctcga cgccatcatg
     9241 gcgttcttcg ccgaggactg ctcgctcgat atgcctcggg gaccggagcc ctggggccag
     9301 cgctgcgccg gcaaagcggc cgtgcgagag ggattggcga cacgcttcaa gggtctcccc
     9361 gacgtccact atagcgacga ccggcattgg gtcagcggca acaagggcgt ctcggaatgg
     9421 ctgctgaccg gcacgactcc cgagggtgtc ccggttcggg tacgcgggtg cgatcactgg
     9481 gagttccggg acggaaaagt gatcaggaag gactcgtact ggaagatcgt tgagaagcgc
     9541 gcatgaatac gccctgccgg taacaatcgg gcgaggttcg agcgctcctt tgatcgctgc
     9601 tgcaaccgtg cccggaccaa tcgctcactt ttgaccccgt ttctcccgct ctccgacccg
     9661 gcttttcgct ctgcgctctt gctacttcat cgcgcaggcg cccatcatgg gaacgcaagt
     9721 tccattgagg gctgaccaat gctcaagggt ttcagggatt tcctgctgcg gggaaacgtc
     9781 gtggacctgg cggtggccgt ggtgatgggc gtcgcgttca cggcgctggt caattccatc
     9841 gtgaccaacc tcatcacgcc gatcatcgca ctggccggcg gcgcgcccga cttctcggca
     9901 atccggaccg gtccgatctt gtggggcaac atcctcaacg atgtgctcac tttcgtgata
     9961 accgcggccg cggtctattt cgcgctcgtg atgcccatca acgccctgat ggcacgccaa
    10021 cgcgcgccag ccccatcagc ggcctcgacc cggaagtgtc cggagtgctt gagtgagatt
    10081 ccgataggag cccggcgatg cgcctattgc agtgtgcaga tttccagagc cgccggttga
    10141 ctgcacccaa agctgaaggg gcgccagctc gagccggcgc ccgacgtcga gcgcggtttg
    10201 ctcgcgtcaa gaaaatcgcc atcgctcctg atcaccggag tcgattcgcc tccgtcaatg
    10261 aggcacctgt tcatcgtcag acgaccgtca aggagagcac cgagtgcgaa agctgatcat
    10321 gtggaacctc attaccctcg atggttactt cgagggcgag aagagctggg acttgccttg
    10381 gcacgaacgc gtgtggggcg aggaattgga gcggttttca ctcgaccagc tgcagtcggc
    10441 cgacatgctg gtctttgggc gcgtgacgta tgaaggcatg gcggcgtact ggcagacggc
    10501 gacgggcgcc atcgcggact acatgaacag cctgcccaaa gtggtgtgct cgcgcactct
    10561 ccagtctgca ggctggaaca attccacgtt ggtgcgacaa gacgcagcaa aggaactggc
    10621 aaagctcaag caacagggca acggggacat gtttgtcttc ggcagcgcca acctctccga
    10681 gaccttcatg aacgagaacc tcttcgacga gtatcgcatt ggaattgcgc cggtcgttca
    10741 tggccgcggc aggcttttgt tcagccgcgg atcgaggccg cagtggcttc agttgctcga
    10801 agcacgtccg ctttccaacg gttgcctcat tcttcggtac aaaagcgaag cggcggcatg
    10861 aatggttctt ccggcaccgg gtcatatgac aatcggccgc agcggacggc ccgctacgcg
    10921 gcccgccgtt catggctccc ttaaagtcag gaggacgtga tgcgacctct tcggtattcc
    10981 atcaacgtta cgttggacgg ctgctacgat catcgtgcaa tgatccccga cgaagagttg
    11041 catcgtcacg cggccgaggg catcgcgcag gccgatgccc tcctcttcgg ccgggtaatc
    11101 tatgaaatga tggagtcggc gtggcgcgag ccggcggaga cgggagtgct gcccgattgg
    11161 atggaaccct ggatggtgcc cttcgcccgg acgatcgacg cggcccggaa gtacgtggtg
    11221 tcgagcacgc tcgagcgggt cgattggaac gcggagctcg tgcgcgggga tctggcgcac
    11281 gccgtccggc ggctcaaaca ggagccgggc aagggactcg ccacgggcgg tgtgacgctc
    11341 ccgcaggcgt tggcggagct gggactgatc gacgagtacg agttcatcgt acatccccgg
    11401 ctggcgagcc atggaccgac gttgttcgcg ggactgtcga aacatgtcga cctgaagctc
    11461 gtcgaccggc tcgagttccg ctcgggggcg gtggtcctgc gatatgtgcc gaaagggtag
    11521 cgatcaggcc ggttggcacg aagcgggctg cagcacgcaa cgcctcaacc cttgcgactt
    11581 atcttcccca ataaaagtaa aaggggacag actgaggtgc ccccacttcc acggacacgc
    11641 ggttaaggaa cggctcgctc ttcatacgcg accggggcga cgtagtcgag cgtctgatgc
    11701 aggcggcttc ggttatagaa gagctcgatg tagtcgaaga tcgcccggcg ag
//
LOCUS       DASVDU010000133         4124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1429578, whole genome shotgun sequence.
ACCESSION   DASVDU010000133 DASVDU010000000
VERSION     DASVDU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429578"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VF226_08415"
     CDS             complement(<1..1005)
                     /locus_tag="VF226_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7074047.1"
                     /translation="MPSFFIDRPIFAWVVAIFIMLAGVIAIPLLPVSQYPKVAPPQIS
                     VTANYPGASPEDMYLSVTRPIEEELNGVPGLIYFESTSDSSGRINITATFAPGTPIGE
                     AQVEVQNRIRRVESRLPQAVVDQGMRIEQAGSGFLLVIALRSPDGSYDNVGLGDYLNR
                     NVIGEIRRIDGVGSATLFATQRSMRIWMDPDKMLGLNLTAGDIISAIRAQNAQVAAGR
                     IGAQPNPITQQISATVLVKGQLTSPEEFESIVLRANPDGSLVRLRDVATVEVGGESYN
                     FSTRLNRQPTAGIGVQLSPTGNALATSKAIHAKMEELSRFFPPGLEYSIPYDTSPFVE
                     I"
     gene            complement(1029..2219)
                     /locus_tag="VF226_08420"
     CDS             complement(1029..2219)
                     /locus_tag="VF226_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7074048.1"
                     /translation="MSVLSSFRYGLVAVGLFTLSAQAIAADPGASPPPVTVSIITTEK
                     QDLPIIAELPGRIAPMRIAEVRPRVSGIVIERVFTQGSLVKEGDVLYRIDSAPFQVQV
                     DSAKATLRRAEAAQLRARQQADRQKELRERNITSPQQLEEALATLAQADADVAAAQAG
                     LTAAELNLQYTEVRAPISGRIGRALITEGALVSSSDKLATIQQLDPVYADFTQSVNAL
                     RALRRAVKEGTVSSISEDEMKVQLRFDDGTEYPHPGRLLFSEVTVEATTGQVILRGQF
                     PNDEGELLPGMYVRVLVEQGVQHDAIAVPQQAVQRDGSGRSQVYVVKPDGVLELRTVR
                     TGRVIGNQWVIEEGLAAGERVVVEGFQKIRPGATVNVQAWKTDASAPTRTANRANAAT
                     QGAQ"
     gene            complement(2295..2873)
                     /locus_tag="VF226_08425"
     CDS             complement(2295..2873)
                     /locus_tag="VF226_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7074049.1"
                     /translation="MGRLKTIDRESVLCAAERVVSREGAARLTIEAVATEAGISKASV
                     LYDYKTKQALIRAVIERRMKAECARLAAAIEDAGPGPDRAMRGRLAVAQSRSMSDADR
                     AVALNLIAALAQDEELRRPMQEAYRQQIAEIMETSTHPRGALLAFLALEGLKVLECFG
                     IYSWPEKDRRRLFSDISALLRGELAPAQGDAH"
     gene            complement(3060..4022)
                     /locus_tag="VF226_08430"
     CDS             complement(3060..4022)
                     /locus_tag="VF226_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074050.1"
                     /translation="MRAFARRSTADHPDYDGFDDPFEAHDHFSSFELSPGERVCPPPS
                     RSGGGAKFAGALLLLIALGCGWAFYGNPGSLEGLLPEKVASLISELRGKPQFGSEMLE
                     QAFAESPMAPEDKDASQDRLPRIEESAPAAIESATTKPLDRIEAELKDNGKPEAGGTP
                     PAEGPGEATGSAKEPVTINTAAVPEQAEERDEPEVTPLPPADPDPADPLQMRAAEVGL
                     HPGLSRVLLSKLTATDYRNAAIAIKTALAKTADDAVYVWPRQRTPELALFKVHFVPGA
                     APGCRRYVVTITKDGWSTTALPMENCDSRPSRSVASRSAHAARR"
BASE COUNT          697 a         1356 c         1333 g          738 t
ORIGIN      
        1 gatctccacg aacggggagg tgtcgtaagg aatgctgtac tcgaggcccg gcgggaagaa
       61 gcgggacagc tcctccatct tggcgtggat cgccttcgac gtggcgagcg cgttgcccgt
      121 cggcgaaagc tgcacgccga tacccgctgt gggctggcgg ttgagccgag tggaaaagtt
      181 gtagctctcc ccgccgacct ccacggtggc gacgtcccgc agccggacga gcgagccgtc
      241 cggattggcg cgcaggacga tggactcgaa ctcctcgggc gaggtcagct ggcccttgac
      301 cagtacggtc gcagagatct gctgcgtgat cgggttcggc tgcgcgccga tgcggccggc
      361 cgccacctgc gcattctggg ccctgatggc cgagatgata tcgccggccg tcaggttgag
      421 acccagcatc ttgtccgggt ccatccagat gcgcatcgag cgctgggtgg cgaacagcgt
      481 tgcgctgccg acgccgtcga tgcggcggat ctcgccgatg acgtttcgat tgaggtagtc
      541 gccgaggccc acattgtcgt aggacccgtc cggtgaacgc agggcaatca ccagcaggaa
      601 gcccgagccc gcctgctcga tgcgcatgcc ctggtcgacg acagcctgcg gcaggcgtga
      661 ctcgacgcgc cggatgcggt tctgcacctc gacctgggcc tcaccgatcg gcgttcccgg
      721 tgcgaacgtc gccgtgatgt tgatacggcc cgaggagtcc gaggtcgact cgaagtagat
      781 gagcccgggg acgccgttca gctcctcctc gatcggccgg gtgacgctca gatacatgtc
      841 ctccggcgac gcgcctgggt agttcgccgt gaccgaaatc tgcggcggcg ccacctttgg
      901 atattgcgag accggcaaca gcggaatcgc gatcacgccg gccagcatga tgaagatggc
      961 gacgacccac gcgaaaatgg gccgatcgat gaagaaactc ggcatctgga atctgccttg
     1021 ctgctcgctt actgggcgcc ctgagtggcg gcgttggccc tgttggccgt ccgcgtcggc
     1081 gcgctcgcgt cggttttcca agcctgaacg ttgacggttg cgccagggcg gatcttctga
     1141 aagccctcga ccacgacgcg ctcgccggcc gccaagccct cctcgatcac ccactggttc
     1201 ccgatgaccc ggcccgtgcg caccgtgcgc agctccagga ccccgtcggg tttcacgaca
     1261 tagacctgcg agcggccgct gccgtcgcgc tgaacggcct gctggggcac agcaatggca
     1321 tcgtgctgca cgccctgctc gaccaggaca cggacgtaca tgccgggcag cagctcacct
     1381 tcgtcgttcg ggaactgccc gcgcaggatg acctgccccg tcgttgcctc gacggtcacc
     1441 tccgagaaca gcagccggcc ggggtgcgga tactcggtgc cgtcatcgaa acgcagctgg
     1501 accttcatct cgtcctcgct gatgctcgag acggtgcctt ccttcaccgc ccggcgcagg
     1561 gcacgaagcg cgttcaccga ctgggtgaag tccgcataca cgggatcgag ctgctggatc
     1621 gtagccagct tgtctgagct gctgacgagc gcgccttccg tgatgagcgc acgcccaatg
     1681 cggccggaga tcggcgcccg cacctccgta tattgcaggt tcagctctgc cgcagtcagc
     1741 ccggcctggg ccgccgcgac gtcggcatcg gcctgcgcca gtgttgcaag agcttcctcg
     1801 agctgctgtg gactggtgat gttgcgttca cgcagctcct tctggcggtc tgcctgctga
     1861 cgagcccgaa gttgcgcggc ctcggcccgg cgcagcgtcg ccttggcgct gtcaacctgc
     1921 acctgaaacg gcgccgagtc gatccggtag agcacatcgc cttccttgac gagactgccc
     1981 tgcgtgaaga cccgctcgat cacgatgccg gagacgcgcg ggcgcacctc cgcgatccgc
     2041 atcggcgcga tgcgccccgg aagctcggcg atgatcggca ggtcctgctt ttccgtggtg
     2101 atgatgctca cggtgaccgg cggcggactg gcgccgggat cagcagcaat cgcctgggct
     2161 gaaagggtaa aaaggccgac cgcgaccaat ccatacctga acgatgaaag gacggacatc
     2221 gatgaattcc tgattttttc agtacgacga agacagacgc cagaaggcgc cagtcaatcg
     2281 cgcgctcagc cagcctagtg cgcatcgccc tgagcgggcg ccagctcccc cctaagaagc
     2341 gccgagatgt cgctgaacag tcgcctgcga tccttctccg gccaggagta gatgccgaaa
     2401 cactcgagaa ccttcaagcc ctcgagtgcg agaaaggcca gcagtgcccc tcgggggtgc
     2461 gtagacgttt ccatgatttc ggcgatttgt tggcgataag cttcctgcat ggggcgccgc
     2521 agctcctcat cctgagcgag agcggcaatg agattgagcg ccacggccct gtccgcatcc
     2581 gacatcgacc ggctttgcgc aacggcaagg cggccgcgca tggccctgtc gggccccggc
     2641 ccggcatcct cgatggccgc cgcgagccgc gcgcactcgg ctttcatgcg ccgctcgatg
     2701 acggctcgga tcagagcctg ctttgtcttg tagtcataga ggacgctggc tttgctgatg
     2761 cccgcttcgg tcgcgacggc ctcgatagtc aggcgtgcgg caccttcgcg gctgacgacg
     2821 cgttcagccg cgcaaagcac agactcccga tcgatggttt tgaggcgccc cattcgagct
     2881 cctgattttt aaccgtccgg tcggttaagc ctagggatgc cgcttgtcaa ctgggcactg
     2941 tgacaggcac cacacgacgc acggcctggg gggccgggtt gaacgacggc gagatgtcct
     3001 tggagctgcc ggaacgctgc ggggctctat ccaaatggtg agtatcagga gccggaccgt
     3061 caccggcgcg ccgcgtgcgc ggacctgctc gccacggatc ggctcggcct cgagtcgcag
     3121 ttctccatcg ggagcgccgt ggtcgaccag ccgtccttgg tgatggtcac gacgtagcgg
     3181 cggcagcccg gggcggcacc aggaacgaag tgcaccttga acagagcaag ctcgggcgtg
     3241 cgctgccgcg gccagacgta gacggcgtcg tcggcggtct tggcaagtgc ggttttgatc
     3301 gcgatggcgg cgttacggta atccgttgcc gtcaatttgc tcagcagcac acgtgacagc
     3361 cccgggtgaa gtccgacctc ggcagcccgc atctgcaggg gatcggccgg gtccgggtcc
     3421 gctggcggca gcggcgtgac ctccggctca tcccgttcct cggcctgctc cggcaccgcg
     3481 gcagtgttga tcgtcaccgg ctccttcgcc gatccagtcg cctcccctgg cccctcggcc
     3541 ggcggcgttc cacctgcctc cggctttcca ttatccttca gctccgcctc gatgcggtcc
     3601 agtggcttgg tcgtagccga ctcgatggcc gccggcgcag actcttcaat tctgggaagc
     3661 ctgtcctggg aggcatcttt atcctcgggc gccatagggc tctcggcgaa agcctgctcc
     3721 agcatctcag acccgaattg aggttttccg cgaagctctg aaatcaggga tgcgaccttt
     3781 tctggcagta atccttccaa agagcctgga ttgccgtaga acgcccagcc acacccgagc
     3841 gctatcagca agagcaaagc accagcgaac ttggctccgc cgccggagcg gctgggcggc
     3901 gggcacacgc gctcgcccgg cgacagctcg aaggacgaga aatggtcgtg agcctcgaaa
     3961 ggatcatcga aaccgtcgta gtcagggtga tctgcggtcg aacgccgggc aaaggctctc
     4021 atggctgccc ctcgacatga cttcccgtgg atccccgcag ttgcgcaagc atcctcaaat
     4081 cattggcgaa ataagggcgc atggcgggcg ccggattccg cact
//
LOCUS       DASVDU010000134         9026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1359278, whole genome shotgun sequence.
ACCESSION   DASVDU010000134 DASVDU010000000
VERSION     DASVDU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9026
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359278"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VF226_08435"
     CDS             complement(<1..621)
                     /locus_tag="VF226_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074051.1"
                     /translation="MNEQLRRTSAVEDFAGAGDPGALEAMYDASRRRAPTLSPTEAAL
                     VQAYRDRGFLRSRLDPLGLAPLPTVPELDPGRYGLDPARSRPLIEQMEAAYCGPIGWE
                     FGHIHDAARRQWLAAEAEQGARFPDPAARQSMLALLTRAQTFETVLAQRLPGARLFGL
                     GGAESFIVLLDVLLAESVPLGLQEVVIGGMHRGRFNLLANVCGKPLG"
     gene            728..1951
                     /locus_tag="VF226_08440"
     CDS             728..1951
                     /locus_tag="VF226_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7074052.1"
                     /translation="MPAPLSHIRVLDLSRVLAGPWASQFLADMGAEVIKIERPRSGDD
                     TRAWGPPFALEPTENDPGISAYFACANRGKQSVTVDIAHPKGQELIRKLAARSDVLLE
                     NFKVGGLAKYGLDYESLKAINPRLVYCSITGFGQTGPYAPRAGYDFLVQAMGGLMSIT
                     GEPDDVPGGGPVKVGVAISDQMAGMNALAAILTALLKRERTGEGEYLDVALLDSTVAA
                     LINQASTYLVSGTVPQRMGNAHPTVVPYQAFQTADGHIILAIGNDGQFQRFCAAAGLE
                     ALAEDARYRTNAARIINRETLIPRIAEALRKRTSSDWIALLESKAVPCGPINTIDQVF
                     ADPQVQARGMRRVLNHETAGPLPVVANPVRMATHDTTSAKAPPMLGESTDTVLSGVLG
                     LSADEIAALRAEGIV"
     gene            complement(1966..2292)
                     /locus_tag="VF226_08445"
     CDS             complement(1966..2292)
                     /locus_tag="VF226_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HEX7074053.1"
                     /translation="MLLSEIITSLLVLVGGAFALLGSIGLARLPDFFSRLHGPTKATT
                     LGIGSVVIASLVNTAGGEFGLGLRELAITLFLFITAPVSAHMLAKAALSQERRRREAE
                     DAGEQP"
     gene            complement(2298..2567)
                     /locus_tag="VF226_08450"
     CDS             complement(2298..2567)
                     /locus_tag="VF226_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEX7074054.1"
                     /translation="MLSYASAAAIGMVITAMLLNLFRLVRGPSLPDRVLALDTLSINT
                     IALVMLFGISIGSEVYFEAALLIAMMGFVSTAAFCKFMLSGDVIE"
     gene            complement(2561..3052)
                     /locus_tag="VF226_08455"
     CDS             complement(2561..3052)
                     /locus_tag="VF226_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX7074055.1"
                     /translation="MIDRLLPRPILSMVILVLWLVLASSVGIGQVLLGAIIAITIPIA
                     TASFWPEPIKVVRPLAGLRLFVVLLWDILVANVRVARLVVGPLDRLRPQFIEVPLDLD
                     DRLVSVILGSVVALTPGTVSVDIDRRRHTLLVHALDVEDQQKLIDTIKTRYEAPLKET
                     FGC"
     gene            complement(3049..4581)
                     /locus_tag="VF226_08460"
     CDS             complement(3049..4581)
                     /locus_tag="VF226_08460"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX7074056.1"
                     /translation="MSHALILPILLPMLAAIVCIAGYRRGLAFQRKVSLAATLLLPIV
                     SALLVASAARGEISVYAIGSWQAPFGIVLVLDRLSAAMVMLTAVLAVPVLLYALAGMD
                     AKSRNFHPLFQFQLMGLNGAFLTGDIFNLFVFFEVLLAASYGLLAHGGGLLRARASLV
                     YVVLNLVGSTLFLVALALVYGTLGTLNFADLSLAVPHVPPSDQALVRMAFALLVVVFA
                     LKAALVPLSFWLPLAYGAATAPAAALFSILTKVGIYALLRLSGLVLASASFTASLLQP
                     WLVQLAIATIAISTFGILAASRLSVVVANLVMLSTGALLSVMAVPGIEGVSATIYYVL
                     HSTLVTGGLFLLADAIANQRGAHGDLLERGPQVRDLGVLSGAFLVLAVAVTGMPPLSG
                     FFGKFMLMKSVQATGWAAAIWTALLLSGLLAALALARQFSVLFWEPVPDEPKPEYPPP
                     SRLRVAGLAILVAASPVLTLLTGTVSSYAHAAAEQLLSRQAYVDAVLGASPDITRSRR
                     PK"
     gene            complement(4578..4910)
                     /locus_tag="VF226_08465"
     CDS             complement(4578..4910)
                     /locus_tag="VF226_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX7074057.1"
                     /translation="MELLLSVGIGVLTASGIFLILRARTFSVVLGSTLLSYATNLVIF
                     ASGRLMIGAPPLILPGQSGHTDPIPQALVLTAIVISFGMTAYIVGLALRAKGESGTDH
                     VDTEEPPR"
     gene            complement(4912..7713)
                     /locus_tag="VF226_08470"
     CDS             complement(4912..7713)
                     /locus_tag="VF226_08470"
                     /inference="COORDINATES: protein motif:HMM:NF009288.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEX7074058.1"
                     /translation="MTQVFLPLTILILFAAIPIVVIASRQSRGAAAWITAGAMALSLA
                     MVVPLFVSSQGQGQTALIVRWDWLPEWGVSVALRLDGLTLLFAILVLGIGILVVLYAT
                     YYLPGSDPLGRFFGLLLGFAGAMLGLVLSEDLIQLAVFWEMTSIASFLLIAYKHQAHD
                     ARIAARMSLAVTGAGGLALLAGVMVLGSIAGSFEISRVLSSGELIRSDRLYPLALVLI
                     LIGAFTKSAQFPFHFWLPNAMAAPTPVSAYLHSATMVKAGVFVLARFYPVLSGTDLWV
                     ALVFPVGATTMAYGAYVAMLQNDLKGLLAYSTISNLGLITLLFGLDTAMSAIAGVFHI
                     VNHAIFKGSLFMAAGIVDHECGTRDMRRINGLIKYMPYTAMLGIVAAGAMAGVPLLNG
                     FLSKEMFFAEVVELRSWFLPIFATIAGILTVGYSARFIYDVFFGGEPVNLPRQPREPV
                     MWIRIPVDVLVLLCLVVGLFPQYTVQYLLQSAAMVVSNGPVPQFELAVWHGFTPPLLM
                     STIAIVGGIIYYSQSDHVIALHERYLPRLHSRIAYERFYRGLVEASKVVIAAIDNGSL
                     QRYLALLFGSVLVLGGWAYLTGAPAPVRGAEAGLAPDAVTAAGLLALVVGSLGTVIYH
                     RQRLKALVYLSVVGLVVSLAFVRFSAPDLALTQLSVEVVTIILLLLSLRFIPREQPAE
                     STYSRRIRDFGLAVAGGTGVGLVVYAMLTRPFSTISDYYLQNAFSEGGGTNAVNVILV
                     DFRGFDTFGEITVLSLVAVALVALLDGLRLRPYPPPAASEADRHPIMLAMLMRPLLPL
                     ALVVSLYILLRGHNLPGGGFIAGLICGVALILQYVASGIDLAQARLRLDFLWVLAAGL
                     ALAVGTGLGSILFGYPFLTSTFGHIHLPLIGDVELASAMLFDLGVFLVVAGVVLVIMS
                     RFGVLSRRELPRGGAKGER"
     gene            7906..>9026
                     /locus_tag="VF226_08475"
     CDS             7906..>9026
                     /locus_tag="VF226_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HEX7074059.1"
                     /translation="MNDHDRSRFEQNRLEANLKKSALDYHEADPPGKITIQATKPVAN
                     QRDLALAYSPGVAHACNAIVDDPAKASRLTARGNLVGVITNGTAVLGLGNIGPLASKP
                     VMEGKAVLFKKFAGIDVFDIEIAETEVEPFVEAVARLEPTFGGINLEDIKSPECFEIE
                     RRLQERMRIPVFHDDQHGTAIVVAAGILNGLKLVGKDISEVKLVCSGAGAAALACLNL
                     LVSLGMRRENIFVSDIKGCVYKGRTELMDAYKEPFAQDTKARSLAELMPGADIFLGLS
                     AGRIVTQEMVAQMAPQPLIFALANPVPEIMPEDVKAVRPDAIIASGRTDYPNQINNVL
                     CFPFVFRGALDVGATTINEEMKKAAVLALASIVEAEASD"
BASE COUNT         1914 a         2909 c         2866 g         1337 t
ORIGIN      
        1 cccgagcggc ttgccgcaga cgttcgcgag caggttgaag cgcccgcgat gcatgccgcc
       61 gatcaccacc tcctggagcc ccagcggcac gctctcggcc aagagcacat cgagcagcac
      121 gatgaagctt tccgcacctc cgaggccgaa gagccgggca ccaggcaatc gctgcgccag
      181 tactgtttcg aaggtttgcg cccgggtcag caaggcaagc atggattgcc gcgcggcggg
      241 atcgggaaat cgggcgccct gctcagcctc ggctgcgagc cattgccgtc tcgccgcatc
      301 gtggatgtgg ccgaactccc agccgatcgg cccgcaatag gccgcttcca tctgctcgat
      361 cagcggccgc gacctcgccg gatcgagacc gtagcggccg ggatccagct ccggcacggt
      421 aggcagtgga gccaaaccca gcggatcgag ccggctcctc aggaagcctc gatctctata
      481 ggcctggacg agtgccgctt ctgtaggtga cagggttgga gccctacgcc gcgatgcatc
      541 gtacatcgcc tcaagcgcgc cagggtcgcc tgcaccggcg aaatcctcca cggctgaggt
      601 gcggcgtagc tgctcattca tttccgactg caatccctgg acactctgcc cctcatccgg
      661 caggacaaga ctttgcccgc gttcgtgcta accgttagcg cacaacgcaa ccccgggaaa
      721 cgacttcatg cctgctccgc tctcgcatat ccgcgtcctc gacctgtctc gcgtcctcgc
      781 cggcccctgg gcctcgcaat tcctggccga catgggcgcg gaagtgatca agatcgagcg
      841 cccccggagc ggcgacgata cccgcgcctg ggggccgccc ttcgcgctgg agccgaccga
      901 gaacgaccca ggcatctccg cctatttcgc ctgcgcgaac cgcggcaagc agtcggtgac
      961 ggtcgatatt gctcatccca agggccagga gctgatccgc aagctcgccg cccgatcgga
     1021 cgtgctgctg gagaacttca aggtcggcgg gctcgcgaaa tatggcctcg actacgaaag
     1081 cctgaaggcc atcaacccgc gtctcgtcta ctgctcgatc accggcttcg gccagaccgg
     1141 gccttatgcg ccgcgcgcgg gctatgactt ccttgtgcag gccatgggcg gcctgatgag
     1201 cataacgggc gagcccgatg acgtgcccgg gggcgggccc gtgaaagtcg gcgttgccat
     1261 ctcggaccag atggcgggca tgaacgcgct ggctgcgatc ctgaccgcct tgctgaagcg
     1321 ggagcggact ggagaaggcg agtacctcga cgtcgccctg ctcgacagca ccgtcgcggc
     1381 gctgatcaat caggcctcta cttatctcgt ctccggcacg gtcccgcagc ggatgggcaa
     1441 cgcgcacccg acggtcgttc cctatcaggc gttccagacc gcggacggcc acatcatcct
     1501 ggccatcggt aacgacggcc agttccagcg attctgcgca gccgccgggc tggaggcgct
     1561 tgcagaagat gcccgctatc gcaccaacgc cgcccgcatc atcaaccgcg agacactcat
     1621 cccgaggatc gccgaggcgc tccgaaagcg gacgtcgtca gattggatcg cactgctcga
     1681 gtcaaaggcc gtgccctgcg gcccgatcaa cacgatcgat caggtcttcg ccgatccgca
     1741 ggtacaagcg cgcggcatgc ggcgcgtgct caatcacgag accgcaggcc cgcttcccgt
     1801 cgtcgccaat ccggtacgca tggccacgca cgacacgacc tctgcaaaag cgccgccgat
     1861 gctgggggag agcaccgaca ctgtgctgtc aggcgtgctc ggtctttccg ctgacgagat
     1921 cgcggctctc cgcgcagaag ggatcgtctg agcaaccaca acccgtcagg gctgctcacc
     1981 agcatcttcc gcctcgcgcc ggcggcgctc ttggctcagc gcggcctttg ccagcatgtg
     2041 cgcgctgacg ggcgccgtga tgaacaggaa cagcgtgatg gccagctctc gcagccccag
     2101 cccgaactcg ccgccggctg tgttcaccag cgatgcgatg acgacggagc cgatccccag
     2161 tgtggtcgcc ttcgtcggtc cgtgcaggcg ggagaagaag tcaggcaggc gtgccaatcc
     2221 gatcgagccc aagagcgcga acgctccacc gacgagcacc agaagcgacg tgatgatttc
     2281 agacaaaagc atttcggtca ctcgatcacg tcgccgctca gcatgaactt gcagaatgct
     2341 gccgtgctca cgaaccccat catggcaatc agcagcgccg cctcgaaata gacctcgctc
     2401 ccgatgctga tcccgaacag catgacaagc gctatggtgt tgatcgaaag cgtatcgagt
     2461 gcgaggacgc gatcgggcag gctgggtccg cgtaccagcc ggaacaggtt gagcagcatg
     2521 gccgtgatga ccatgcctat agcggcggcg ctggcataac tcagcatccg aacgtctcct
     2581 taagaggtgc ctcgtagcgg gttttgatgg tgtcgatgag cttctgctgg tcttccacat
     2641 cgagcgcatg gacgaggagc gtgtggcgcc gccggtcgat gtccaccgag acggtcccag
     2701 gcgtcagtgc aaccacgctg ccgaggatga ctgaaaccag tctgtcatcg aggtcgagcg
     2761 gaacctcgat gaattgcggg cgcaggcgat cgagcgggcc gacgacgagg cgcgccaccc
     2821 gcacgttggc gaccagaata tcccagagca gaacgacgaa aaggcgtaga cctgccagcg
     2881 gccggaccac cttgatcggc tcgggccaga acgaggccgt cgcgatcggg atggtaatgg
     2941 cgatgatggc gccaagcaga acctgcccga taccgacact ggaggcgagc acgagccaaa
     3001 gaactagaat gaccatgctg agtattggtc gagggaggag ccgatcgatc atttcggcct
     3061 ccgtgatctg gtgatgtctg gcgatgcacc gagcaccgcg tcgacatagg cttgacggct
     3121 cagcaactgc tcggccgcag catgcgcgta ggacgatacc gttcctgtca gcagcgtgag
     3181 caccgggctc gctgcgacca gaatggcgag gcctgccacc cgcagcctgc ttggcggtgg
     3241 atattccggc tttggctcat ccggcaccgg ctcccagaac agcacactga actgtcgcgc
     3301 cagggccagg gcggccagga ggccggagag cagcagggcc gtccagatcg ccgccgccca
     3361 gcccgtcgcc tgcaccgact tcatcagcat gaacttgcca aagaaacccg acaggggcgg
     3421 cattcctgta accgccaccg ctagcaccag gaacgcgccg ctgagcacgc ccaaatcgcg
     3481 cacttgcggg ccgcgctcca acaggtcgcc gtgcgcgcca cgctgatttg cgatggcatc
     3541 ggccagcagg aacaggcctc cggtgaccag tgtcgaatgc agcacatagt agatggtggc
     3601 agatacgcct tcgatgcccg gcacggccat gacggaaagc agcgcaccgg tcgaaagcat
     3661 gacgagattg gctacgacga ctgaaagacg cgacgcggcg agaattccga acgtgctgat
     3721 ggcgatggtg gcgatcgcga gctggaccag ccagggctgt aggaggcttg ccgtgaagga
     3781 cgcactggcc aggacgaggc ccgagagcct gagcagggcg taaattccca ccttcgtcag
     3841 aatggaaaac agcgctgcgg caggcgcggt cgcggcgcca taggcgagcg gaagccagaa
     3901 ggagagcggc accagcgctg ccttcaaagc gaacaccacg accagaaggg cgaacgccat
     3961 tctcacaaga gcttggtcgg acggagggac atgcgggacg gcaagcgaca gatcagcgaa
     4021 attcagcgtt cccagcgtgc catagacgag cgcaagcgcg accaggaaca gcgtcgatcc
     4081 caccagattg agaacaacgt aaacgaggct ggctctcgca cgcagcagcc cgccgccgtg
     4141 ggcgagcagg ccgtaggatg cagccagcag cacctcgaag aagacgaaga gattgaagat
     4201 gtcgcccgtc aggaacgcgc cgttgagccc catgagctga aattggaaca gcggatggaa
     4261 attccggctc ttggcgtcca ttccagccag cgcgtagagc agcaccggaa cggccagcac
     4321 agcggtgagc atgaccatgg ccgccgacag gcggtcgagg accagcacga tcccgaacgg
     4381 cgcctgccag ctgccgatgg cgtagaccga gatctcgcca cgcgccgccg aagcgacgag
     4441 gagggccgac acgatcggca gaaggagggt cgccgccagg gagactttgc gctgaaacgc
     4501 gagtccccgc cggtacccgg caatgcaaac gatcgcagcg agcatcggca gcaggatcgg
     4561 caggatcaga gcgtggctca ccggggcggc tcctcggtgt ccacgtgatc ggtgcccgac
     4621 tctcctttgg cgcgcagagc caagccgacg atgtatgccg tcatgccgaa gctgatcacg
     4681 atcgcggtca gcaccagcgc ctgcgggatg gggtccgtat gccccgactg accgggcagg
     4741 atcagcggcg gcgcgccgat catcagccgt ccgctcgcga aaatcaccag attggtggca
     4801 taggagagca gcgtgctccc gagcaccaca gagaatgtcc gtgcccgaag gatcaggaaa
     4861 attccggagg cggtcagcac gccgatgccg actgacagca ggagctccat atcagcgctc
     4921 ccccttcgcg ccgcccctcg gcagctcgcg cctgctcagc acgccgaacc tcgacatgat
     4981 gacgagcacc acgccggcca ccacgaggaa gacgccgagg tcgaagagca tggcagatgc
     5041 cagctcgacg tcgccgataa gcggtaggtg gatgtgaccg aaagtgctgg tcaggaacgg
     5101 atagccgaac aggatgctcc caagccccgt gccgacggcg agcgccaggc cggcagccag
     5161 tacccaaagg aagtccaatc gcagccgggc ctgcgccagg tcgatgccgc tcgccacata
     5221 ctgcaggatc agcgcgacac cgcagatcag cccggcgatg aacccgccgc cgggcaggtt
     5281 gtggccgcgt agcagaatgt agagcgagac gaccaatgcc agcggcagca gcggccgcat
     5341 cagcatcgcg agcatgatcg gatgccggtc cgcctcactg gcggctggcg gcggataggg
     5401 ccttagccgc aggccgtcga gcagggcgac gagcgcaacg gccaccaggg aaagaaccgt
     5461 gatttcgccg aaagtatcga agccacggaa gtcgacgaga atgacgttga cggcatttgt
     5521 cccgccgccc tcggagaagg cgttctgcag gtaataatcc gagatggtcg agaacggacg
     5581 tgtgagcatc gcatacacga cgaggcccac gcccgttccg ccagccactg ccagaccgaa
     5641 atcgcggatg cgccgtgaat aagtcgattc tgccggctgc tcgcggggaa tgaagcgcag
     5701 gctgagcagc agcagaatga tcgtgacgac ctcgaccgag agctgcgtca gggcaaggtc
     5761 gggggcagag aaacggacga atgccagtga gaccacgagg cccacgacgc tgaggtacac
     5821 cagcgccttc aatcgctgcc ggtggtagat cactgtcccg agggagccta cgacgagcgc
     5881 cagtagaccg gccgctgtga cagcatccgg cgcgagaccg gcctcggcgc cgcgcacagg
     5941 agctggcgcg ccggtgagat aagcccagcc gccaagcacg aggacagatc cgaagagcag
     6001 ggcaaggtag cgctgcagcg agccgttatc gatcgccgcg atgaccacct tcgacgcctc
     6061 gacaaggccg cggtagaacc gctcgtaggc gatccggctg tgcaggcgcg gcaaatagcg
     6121 ttcatgcagc gcgatcacgt ggtccgactg cgaataatag atgatgccac cgacaatagc
     6181 gatcgtgctc atcagcagtg gcggggtgaa gccgtgccat acggccagct caaattgcgg
     6241 gaccgggccg ttcgaaacca ccatcgccgc ggactgaaga aggtattgga cggtgtactg
     6301 gggaaaaagc ccgacgacga gacacagcag gacaagcaca tcgacgggga tgcgaatcca
     6361 catcaccggc tcgcgaggct gacggggcag gtttaccggc tccccgccga agaagacgtc
     6421 gtagatgaac cgtgccgaat atccgaccgt gagaatgccg gcgatcgttg caaagatcgg
     6481 caggaaccag gaccgcagct cgacgacctc ggcgaagaac atctccttgc tgaggaagcc
     6541 gttgagcagc ggcacgcccg ccatggcacc cgccgccacg atcccgagca tcgccgtgta
     6601 gggcatgtat ttgatgagcc cgttgatgcg ccgcatgtcg cgcgtgccgc actcgtgatc
     6661 cacgatgccg gccgccatga acagcgagcc tttgaagatg gcgtgattga cgatgtggaa
     6721 cacgccggca atcgcgctca tcgctgtatc gagcccgaac aagagcgtaa tcagacccag
     6781 gttgctgatc gtcgaatagg ccagcagccc cttgaggtcg ttttggagca tggcgacata
     6841 ggcgccatag gccatcgtgg tcgccccgac cgggaacacc agtgcgaccc acaggtcggt
     6901 gccggagagc accgggtaga accgggccag tacgaacacc cccgctttca ccatcgtcgc
     6961 agaatgcagg tacgcgctga cgggagtcgg cgcggccatg gcgttcggca gccagaaatg
     7021 aaacggaaac tgggctgact tcgtaaaggc tccgatcagg atgagcacga gcgcgagcgg
     7081 gtagagtcga tcggagcgga tcagttcgcc gctcgacagc acccgggaga tttcaaagct
     7141 gcccgcgatg ctccccagca ccatgacccc ggcgagcaag gccaggccac cggcgccggt
     7201 gacagccagc gacatgcgtg ctgcgatgcg tgcatcatgg gcctgatgct tgtaggcgat
     7261 gagcaggaac gacgcgatgc tcgtcatctc ccagaagacg gcgagctgga tcaaatcctc
     7321 cgagaggacc aggcccagca tcgcgccggc aaacccgagc aggagaccga agaagcggcc
     7381 gagaggatca gaccccggca gataataggt cgcataaaga acgaccagga tgccgatgcc
     7441 gaggaccagg atcgcaaaca gcagggtcag gccgtcgagc cggagcgcga cactgacacc
     7501 ccattccggc agccagtccc atctcacgat caaagcggtc tggccttgac cttgagatga
     7561 aacgaagagc ggcaccacca tcgccaggct cagggccatc gcgccagccg tgatccatgc
     7621 ggcggcccct ctgctctgcc ggctcgcgat gacgacgatc ggaatggcag cgaaaaggat
     7681 aaggatcgtg agcggcagga aaacctgtgt catgcgtcct tcaagcgagg agatcacgcg
     7741 atttgctggc gagcggctcc caccaacaga acaagaaatc acgtgcctgc gaccccccgg
     7801 ccacagcttt gcaatcagcc cttcggcgtg caacagataa tggtattccg gatgtcagca
     7861 cgtgaactga tccaggcgtg aaccggcgcc tgaagtgtgg aacccatgaa cgatcacgac
     7921 cgatcccgat tcgagcagaa ccgcctggaa gccaatctca aaaagagcgc cctcgactat
     7981 cacgaagctg acccacccgg aaaaatcacc atccaggcga ccaagccggt cgccaatcag
     8041 cgggatctgg ccctggcgta ttcaccgggg gtcgcccatg cctgtaacgc gatcgtcgac
     8101 gatcctgcga aggcgtcgcg gctgacggcg cgcggcaatc tggtcggcgt tatcacgaac
     8161 ggcacggccg tgctcgggct cggcaatatc ggtccgctcg cctccaagcc ggtgatggag
     8221 ggcaaggctg tcctgttcaa gaaattcgcg ggcatcgacg tcttcgacat cgagatcgcc
     8281 gagaccgagg tcgagccctt cgtcgaggcg gtcgcacgcc tggagccgac cttcggcggc
     8341 atcaatctcg aggacatcaa gtcgcccgag tgcttcgaga tcgagcgccg tctccaggag
     8401 cgcatgcgca ttcccgtctt tcacgacgac cagcacggca cggccatcgt cgtcgcggca
     8461 ggcatcctga acggcctgaa gctcgtcggg aaggacatca gcgaggtcaa gctcgtctgc
     8521 tccggggcgg gtgccgcggc gctggcctgc ctcaatcttc tcgtctcgct cggcatgcgc
     8581 cgcgagaaca tcttcgtctc cgatatcaag ggctgcgtct acaaaggccg cacggagctg
     8641 atggacgcct acaaggaacc attcgcgcag gatacgaagg cgcgctcgct cgccgagctc
     8701 atgccagggg ccgacatctt cctggggctc tccgccgggc gcatcgtgac ccaggagatg
     8761 gtggcgcaga tggcgccgca gccgctgatc ttcgcgcttg ccaaccccgt cccggaaatc
     8821 atgccggaag acgtgaaggc cgtccgcccg gatgccatca tcgcgtcggg ccggaccgac
     8881 tacccgaacc agatcaacaa cgtcctctgc ttccccttcg tgttccgcgg cgccctcgac
     8941 gttggcgcca ccaccatcaa cgaggagatg aagaaggccg cggtcctggc gcttgccagc
     9001 atcgtcgagg ccgaagcttc cgacat
//
LOCUS       DASVDU010000135        27821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1405096, whole genome shotgun sequence.
ACCESSION   DASVDU010000135 DASVDU010000000
VERSION     DASVDU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 27821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27821
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1405096"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1187
                     /locus_tag="VF226_08480"
     CDS             3..1187
                     /locus_tag="VF226_08480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7074060.1"
                     /translation="MQSRGPLTGVRVLEFGQIAAGPFAGSLLADLGADVVKVENPAGG
                     DGMRGWPPLTKSEDGRVYSENFASVNRNKRSIAVDLKNQREVLRLKQLVSKADVIIEN
                     FRPGVLKRLGFGYEDLKPLNPRLVYCSISGYGQQGPYAEKGAFDVTVQGMSGLMSVTG
                     EEGRPPVKCGVPVADFCAGLYGAYTITAALMQARQTGEGAYIDCSMLGALIGVAALQT
                     SEYFGTGKSGGRLGSAHPRNAPYQAYRASDDYFIIAAGNDALWRQVAEAVGLDDLPND
                     PRFRTQLDRARNQAALAEILAERFKTQTAREWLEEMDRRGVPCAPINTYAEILSDPHV
                     LGMGLVRPLTLPNGVETKTTAFPVAISGYAFEVRHAPPELGAHTDEVMTEWLGPQLSE
                     LT"
     gene            complement(1218..1793)
                     /locus_tag="VF226_08485"
     CDS             complement(1218..1793)
                     /locus_tag="VF226_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator RfaH"
                     /protein_id="HEX7074061.1"
                     /translation="MRGDFTGGEELASAHALGEPAIANGAGAVRRWVAVNTHPHREHI
                     ALENLTNQGFDAYCPMVERQVRHARRTRDVRRPLFPGYLFARIDLAGRWRPILSTFGV
                     RSLVRFGDQLSFVEDGLIDGLKAREVDGVIVKPAEPLRPGQQVRVQGGPFDGLVATII
                     EMDEKDRLVVLMDLLNQSVKVKVPTNRVTAS"
     gene            1982..2749
                     /locus_tag="VF226_08490"
     CDS             1982..2749
                     /locus_tag="VF226_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX7074062.1"
                     /translation="MPATAQLTLALEDFDAAVFDLDGVVTKTAAVHAAAWKELFDTYL
                     KARADRTGEPFRPFDIQRDYLQYVDGKPRYEGVRSFLASRSISLPYGSPEDSPDQETI
                     CGLGNRKNEIFNAQLTRTGAEVYQSSLHLIQELRRHGIKTALVSSSKNAKAVLESARL
                     TELFDACVDGIEAARLSLRGKPDPDIYLHAVRLLDVKPARAIGVEDALSGVEAIRAAG
                     YGLVIGVDRGDQADALRDHGADIVVRDLAELQLVEHL"
     gene            2855..5266
                     /gene="ppsA"
                     /locus_tag="VF226_08495"
     CDS             2855..5266
                     /gene="ppsA"
                     /locus_tag="VF226_08495"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX7074063.1"
                     /translation="MAANSDPYVRWLEDLGSDDVGQVGGKNASLGEMIRSLKQEGIRV
                     PDGFATTAYAYREFIAANHLEDAIRDRLEALKAGRATLRETGQAIRRLFLESEFPEPI
                     AQAIRECYRKLAERSGLDDVAVAVRSSATAEDLPGASFAGQQETFLNVRGERALLDAC
                     RRCYASLFTDRAISYRDEMGFDHMQVALSAGVQRMVRSDLAGSGVMFTIDTETGFPNV
                     IIINAAWGLGENVVQGTVNPDKYAIFKPPLRDERYRPIIEKTMGTKEKKMIYAEGGSG
                     RTKNVNTTRREREAFVLSDSDILQLARWAVLIEKHYGRPMDIEWAKDGETGELAIVQA
                     RPETVQAAARSAPLKIYRLKGKQPKPVISGAAIGQAIAAGPVCMIRSAEEISEFRPNS
                     ILVTETTDPDWVPIMRQAAGIVTDHGGTTSHAAIVSRELGVPAIVGTGQATRVLRDGQ
                     DVTISCAQGEIGYVYDGILDFETQEVNPEALPEPRTAMMINLASPATAFQFWRLPTKG
                     VGLARIEFIISNYIKIHPMALVHPDRVEDPEARHQIEQLTRGYGDPRDYFIDQLARGI
                     AKIAAPYHPHPVIVRLSDFKTNEYAHLIGGQSFEPREENPMLGFRGASRYYSDRYRDG
                     FSLECRALRKVREDIGITNVVVMVPFCRTPAEADRVLAIMAEQGLERGKEGLQIYMMC
                     EIPSNVILAEEFAKRFDGFSIGSNDLTQLILGVDRDSAELAPLFDERNEAVKRAVSEA
                     IKKAHKAGIKVGICGQAPSNYPEFAAFLVEQGIDSISLNPDSFIATVTHVAEVERRLA
                     SSGIR"
     gene            5320..6360
                     /locus_tag="VF226_08500"
     CDS             5320..6360
                     /locus_tag="VF226_08500"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7074064.1"
                     /translation="MNGNDKIRVAVNGYGVIGKRVAAAVAQQDDMELVGISDVVSDWR
                     ARAAIIRGFKLFGATAQSAEAMKQAKLDVSGTLDDLLGTAEIVVDCTPKKIAATNVER
                     YRERGVKFVLQGGEKHQVTGHSFVAESNFASAVGRESTRVVSCNTTSIVRTLAALKRA
                     GLLKKARGTLLRRATDPWESHQSGIMNTLVPEPDIPSHQAPDAQSVDPELDVVTMAVK
                     VPETLAHMHYWAVQLTRPASKDEVLEAFRTSSRIAFIRMRDNLVAINAVKEMMADLGR
                     PHDNLYEVALWEDMLKVQDDELFYAYMVDNQAIVIPETIDAIRALSGREKDWQASVKK
                     TNASLGIGLLNW"
     gene            6377..7315
                     /locus_tag="VF226_08505"
     CDS             6377..7315
                     /locus_tag="VF226_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HEX7074065.1"
                     /translation="MPAICTLTLNPALDIATATDRVTATHKLRCTTARYDPGGGGINV
                     ARVIFELGGNATAVYTAGGPVGHSLQELLDAAGIPQVVIPIRGETRVNITVDERVSNN
                     QFRFVFPGPELSPEEQEQCIEAVAKLDPPPEFLVASGSLPPGVPADFYARVARLAKSL
                     GAKYFLDTSGDALREAGHEGIYLVKPNLRELSEFAGRDLASRSDQIDAARELIAEGRA
                     EVVVLSLGADGALLVTNELAEHFPALQVPLRSAVGAGDSMLGGIVYSLARGNPLRDAV
                     RYGIAAGAAALMTEGTELNRREDTERLYAEMLSEAG"
     gene            complement(7334..8029)
                     /locus_tag="VF226_08510"
     CDS             complement(7334..8029)
                     /locus_tag="VF226_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7074066.1"
                     /translation="MQAAQVMTFGAATIQPDASIREAARMMLDYRISGLPVVDQGGNL
                     VGIITEGDLLTGITGDGQRRRWLEFILDPEKPALAAISPRAQTVGEAMTRQVITATEE
                     TPVQDIVKLMKANGIKRVPIVRDNKVVGIVSRADLLRGLALEAKMMPDATAEDYALRD
                     QVLQALSGEARGSRTSINVLVQNGTVELRGALPDASLLEKLVSAARQVPGVKDVVNRL
                     VVVRAHDCASSDA"
     gene            8170..8451
                     /locus_tag="VF226_08515"
     CDS             8170..8451
                     /locus_tag="VF226_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074067.1"
                     /translation="MITVERVSDAPLTFEVSVREGGSETRHRVTMADGMVAQLGQGQF
                     TPEQCIEASFRFLLDREPKESILREFDVAVIARYFPEYEAELPRYLSDR"
     gene            complement(8492..9592)
                     /locus_tag="VF226_08520"
     CDS             complement(8492..9592)
                     /locus_tag="VF226_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074068.1"
                     /translation="MEGLRVELSQERPIPLAAQFSCAPGEVLALVGPSGSGKSTILRA
                     IAGTYRPQLGKIEVNGEAWFSAAAGRFVPAHQRAVGMVFQTYALFPHLTALGNIMAAM
                     THVPAKERAAKARGLLAQVHLAGLEQRRPAELSGGQQQRVAVARALAREPKVLLLDEP
                     FSAVDKATRQRLYREIAELRRGLNMPVVLVTHDLDEALMLADRMAVLHRGRTLQTGTP
                     AEITTRPVSAEVARLVDLRNIFEGRIVTGGTAGKPSIIAWAGLEIEAPARPDLQPGQP
                     ITWVIPDGFVVLHRRDRPSRGEHENPVPGIIEAVLPIGQTAHLTLRPNHDRNLPIHFS
                     VPLHVARRNGVEPGVAASVSLLAEGIHIMPPS"
     gene            complement(9598..10266)
                     /gene="modB"
                     /locus_tag="VF226_08525"
     CDS             complement(9598..10266)
                     /gene="modB"
                     /locus_tag="VF226_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEX7074069.1"
                     /translation="MDWTALFLSLRLAFFTILLLLPIGIFAGRALAYRQFPGKGLVEA
                     IVALPLVLPPTVLGYYLLVAFGAASPMGQLWQALFGGQLVFTFEGLLIASVIFNLPFA
                     IQPMQRGFEAIPSDVREAAACCGLSPMQALRKVELPLAWPGILTAMVLSFAHTLGEFG
                     IVLMVGGSIPGETKTIAIAIYDRVQAFDDMAAGRMSALLLSISLATIALTYAFSRRVG
                     RRLG"
     gene            complement(10289..11101)
                     /gene="modA"
                     /locus_tag="VF226_08530"
     CDS             complement(10289..11101)
                     /gene="modA"
                     /locus_tag="VF226_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7074070.1"
                     /translation="MNLLSRRVALGLSLALVAFGASPVGGATAQNGSANVPIIAAAAS
                     LQFALEEIAEGFKSETGQSVRLSFGSSGNFARQIRQGAPYEMFLSADEDYVLDLARSG
                     LTRDEGAIYAVGRIVIYVPHGSLLKPDASLDDLAAALDDGRIKRFAIANPEHAPYGKR
                     AEEALRHKQLWDRIRPKLVFGENISQAAQFATSANAQGGIIAYSLAVSPHLSQLGTFA
                     LIPEQWHTPLRNRMVLLKTAGPVAERFYAYLQEEQARRVFRKYGFVLPGEAS"
     gene            11263..11691
                     /locus_tag="VF226_08535"
     CDS             11263..11691
                     /locus_tag="VF226_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEX7074071.1"
                     /translation="MTFSPHPKAPGLEVGVTEPMIRELVHTFYARVRRDELLGPIFNT
                     HVADWPEHLDRLCAFWSSVTLMTGRFKGKPVPVHAKLPGITVPHFARWLALFARTARE
                     VCPPEAAELFIDRSERIAQSLQHAIAICRGEPLEVHHDAA"
     gene            11678..12244
                     /locus_tag="VF226_08540"
     CDS             11678..12244
                     /locus_tag="VF226_08540"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7074072.1"
                     /translation="MMPPEVAEWRKAQRDRLLETRRNMPLSERAALIAPLLANLRDLL
                     ETLSFNCLGIYWPIKREIDVRPLGDEICRSRGATLALPVVVERGAPLEYWKWQMGDAV
                     ERGFWNIPVPKQRRPVEPDTVIAPLVGFHDHYRLGYGGGYFDRTLAKLRPFAIGIGFE
                     FSRVDGFVPQPHDIAMNVIVTESAIYQK"
     gene            12265..12693
                     /locus_tag="VF226_08545"
     CDS             12265..12693
                     /locus_tag="VF226_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7074073.1"
                     /translation="MLGISNFHEVFLSTMPDGVLLADKDGVIRMWNAGCERIFGFTAE
                     EAIGQPLDIIIPENLRARHGEGYAQTMRTGKTRYGQGDLLSVPALHKNGTRISVEFSI
                     LPFRDAEGRMVGIGAVLRDVTRKFEEMKALKKALAAARAG"
     gene            12719..13798
                     /locus_tag="VF226_08550"
     CDS             12719..13798
                     /locus_tag="VF226_08550"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX7074074.1"
                     /translation="MWPDRRITDLFGVEHPILLAPMAGPSTPELGIAVSEAGGLGCYA
                     CALSTPEQIRTAMSVIRQRTDKPVNLNFFTHTQPVPDADREARWKARLSPYYSELGID
                     PNAPTPTASRAPFDDTACQIVEEFRPEVVSFHFGLPEDKLLQRVKATGAKVIASATTA
                     REAKWLAERGCDAIIAQGAEAGGHRGMFLTDNIASQAGTLALVPQVVDAVDVPVIAAG
                     GIADGRGIAAAFALGAAGVQIGTAYLFCPEAKVSAMHREALRSAHDDDTVLTNVFTGR
                     PARGLINRIIREVGPVSPDAPAFPLAAGALAPIRAAAEAKGSSDFTSLWSGQSAALGR
                     EMPAAELTRKLAREALDRMKALSGT"
     gene            13900..14871
                     /locus_tag="VF226_08555"
     CDS             13900..14871
                     /locus_tag="VF226_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEX7074075.1"
                     /translation="MLRILAALAALTFLQLPAWAEYSEQYFDVPKGDGPHDVAVAPDG
                     TVWYAGQKAGVAGKLDPATGKIERITLGQNSAPHGVIVGPDGAPWFTDGGQNAIVRVD
                     PETHSVKIWPLPPERMPYTNLNTAAFDGKGRIWFTGQNGIYGRLDPETGEMKVWDAPR
                     GRGPYGITATPGGEIWFVSLAGSYLANVDLETGQAAIYEPPTPNQGARRVWSDSQGRL
                     WISEWNTGNVSVYDPASDTWRQWKLPGERPRTYSVWVDPDDKVWLTEWSSNAVVRFDP
                     ETEKFESFPSNRANANVRQMLGRKGEAWAPESGTDRLRVIRYASKTD"
     gene            14878..15288
                     /locus_tag="VF226_08560"
     CDS             14878..15288
                     /locus_tag="VF226_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX7074076.1"
                     /translation="MPRPEIAALLIVVTSVSALPSVAMAAEPGDPVRGERVFQRCYAC
                     HSDEPDEGNLQGPNLYKVLGRPAAVVPGFEYSQAMKQKAAAGLVWDAATLDAFIADPD
                     AVVPGTLMSIPPVRNAQERSDLIAYLARFGRFQD"
     gene            15422..15898
                     /locus_tag="VF226_08565"
     CDS             15422..15898
                     /locus_tag="VF226_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7074077.1"
                     /translation="MRVPLRLTINGTLYDLDVPPWTTLLDLLRDRLDLTGAKKGCDHG
                     QCGACTVLVDGRRILSCMTLAVMQDGRQVTTIEGLASDGTLHPMQQAFIDHDAFQCGY
                     CTPGQICSAVGLINEGHAHTPEEVRELMSGNICRCGAYPNIVDAVMDVMAKEGGGA"
     gene            15895..16911
                     /locus_tag="VF226_08570"
     CDS             15895..16911
                     /locus_tag="VF226_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX7074078.1"
                     /translation="MRPFSYTRAGDVSEAVSEASAGAVKFIAGGTNLLDLMKEDVERP
                     MRLVDINQLPLNRIEDTPDGGLRFGALVRNADAAHDPRIRDRYPLLSSALLAGASPQL
                     RNAATTGGNIMQRTRCYYFYDTATACNKRKPGSGCGAIGGINRLHAILGASDHCIATH
                     PSDMCVALAALNAEVRVATPDGGERVIPFADFHRLPGDTPHIETNLRPGELITAVDLP
                     NEMFNKHYTYLKIRDRLSYAFALVSVAAALHIENGRITDARLALGGVAHKPWRDNDAE
                     RLLIGKSPERQHFQAVADAILRDAKGQGDNDFKIPLARRAIVRALEQAANGTPQSQID
                     KHVA"
     gene            16923..19148
                     /locus_tag="VF226_08575"
     CDS             16923..19148
                     /locus_tag="VF226_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7074079.1"
                     /translation="MATTVHIGEPLNRVDGVAKVTGAARYAAEYPVEGLLYGFAISSP
                     IAKGRITAIDTSAALAVPGIVEVITHLNRPHVAWQDISYRDDASPPGSPLRPLYDEKI
                     YFSGQPIALVVAETPEIARYGASLVAVTYDAEPHNTDFDTGLSERYPPKKVRSHAVPP
                     DDRGDADGALKSAPVRIDGDYRLMMEVHNPIEMHASTAIWEDGGKITVYDKNQGSQNV
                     QTYLSNVFDLPADKVRVLNPYVGGAFGSGLRPQWQVNLAVLAAKMLERSVRVVMTRQQ
                     MFTHTHRPECLMCVSLGADENGKLSAIAVDATSATSRYEDYSESIVKWSGIIYGCENG
                     SFSHELVPLDTSTPGDMRAPGAATGMNLFEIAMDELAYAAGMDPLPLRIANFTDFDLV
                     TNAPYTSKALLAAYREGAARFGWDKRSHAPRSMRDGKELVGWGMASGIWETLLAEIAA
                     RATLSADGSLEIASAASDIGTGTYTIMAQVAGETLGLPANRIRVKLGDSHLPKSPLEG
                     GSWMAASVGAAVQLACRSLAGKIHQIAANMNNPAFGTPTLDQVAFADGAIQLKGDPTR
                     GMRYEDVLKASGQASISAEETYKPHRRRDKTSNAHSAVFAEVKVDEELGVVRVTRIVC
                     AAAAGRILNPKTARSQILGGIVMGQGMALEEETLTDHRIGRIMNHNLAEYHAPVHADV
                     GHIDVIFVDEPDPEVSPLGAKGLGELGIVGTAAAIANAIYHATGKRIRDLPITIDKLI
                     N"
     gene            19497..20588
                     /locus_tag="VF226_08580"
     CDS             19497..20588
                     /locus_tag="VF226_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7074080.1"
                     /translation="MRKLLLAILALVIAAGGAVLLVPGWRAKLHAEKSPKPAQQAHAV
                     SVEVAPAISAVYQEDLRSVGTLMSDESVQVASEIAGRIASFEFKEGQPVSAGDPLIKL
                     DDSLAQAELVDAKARFELARANRERARKLSKSGYTTEKAHDEADAAFETARAALELAT
                     VKLDKHTIRAPFDGIIGLRNASVGAYVSIGTPIVNLEKIDVLKLDFKLPEAALTQITT
                     GQRVLVEVDAVPNRTFPAEIYAISPMLDVNGRALHVRARMKNPDLLLRPGLFARVTVK
                     GRETREVVLVPESAIVPRGGETFVYVIENGKAIETRVTLGKRGDGRVQILSGLDADST
                     VVVAGQLKLRNGVEVEVVQSQTTRQLEKS"
     gene            20588..23665
                     /locus_tag="VF226_08585"
     CDS             20588..23665
                     /locus_tag="VF226_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7074081.1"
                     /translation="MNPVEVCIRRPVFSTVLSLVLVLLGLVSYSRLTVREYPNVDEPT
                     VSVTTTYPGASPNIVETQVTQVLEGSIAGIAGIDVLESTSRSETSRITVRFRQDVNPD
                     EAANDVRDRVSRVRGRLPDEIDEPIIRKVEADAQAVMYIVFTSDQMSALEITDYVDRY
                     VVDRLKNLTGVADVMIYGERRYAMRIWVDRERLAAYALTVQDIEDALRNQNVEIPSGR
                     IESREREFSVLSRTGLTTPEQFRQIVVKRADGYQVKLGDVARVELGAEDERRDSRYNG
                     QPAVTVGIIKQAVANPLDVSKAVRGILPALNESMPNGLKADIGNDDAVFINESIKAVF
                     TTIMEAIVLVVIVIFFFLRSVRASLIPIVTIPISLVATFTLILAFGFSINTLTLLAMV
                     LAIGLVVDDAIVVLENISRHVESGLRPMQAAIKGTREIGFAVLAMTLTLTAVYAPIAF
                     ATGRTGRLFMEFALALAGAVLVSGFVALTLTPMLCSKLLRHNASPGRLFTFIEDVLKR
                     LESGYRRSLEFALSVRWLVVALAILTAGAAGMLFVSLKSELSPTEDRGVLLIRGSGPE
                     GSTLAYNKRYSEQADAILAELPEVRSRLTIDGLYQQVTNFIVIGRLTDWSARDRKQQE
                     LAAEIGPKVRRIPGVLAFAQNPASLGQRGGSRPIEFVIQTSGTYEQLDEYAEKMLERI
                     RSYPGLEGVDTDLKLNSPELRIDVDRAKIADLGLDVAVVGRTLETLLGGRQVTRFEQN
                     GEQYDVIVQLAAEDRSSPETLSTIFVRGANGEMVQLSNIVTVSETVAPKELKRFNQLR
                     SVTIQANLAPGYSLGEGLEFLENTAREVLPSHVQTDLNGQSREFRSAGQSLAFVFLLA
                     LGFIYLVLSAQFESFRSPLIIMVTVPLSMTGALAALHLTGGTLNVYSQIGLVTLIGLI
                     TKHGILIVDFANQQQEAGRDMRTAVLNAAALRLRPILMTTGAMVLGAVPLALATGAGA
                     ESRQQIGWVIAGGMTLGTILTLFVVPVVYMLIGRNYSLIKHEEELADHPQPAE"
     gene            complement(23757..24230)
                     /locus_tag="VF226_08590"
     CDS             complement(23757..24230)
                     /locus_tag="VF226_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007626905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEX7074082.1"
                     /translation="MESAKPQKEHEWLQQLVGDWRFEAPSKDPDCNHTGTETVRSIGG
                     LWIQAEGRSQMPDGQPSVMMLTLGYDPQKEKFVGTWLGSMMTYLWVYEGSLDPTGKIL
                     SLDTEGPSCTEEGKMAKYREVIEIKDPDHRVFTSSVSGADGKWQTIMTAKYTRVK"
     gene            complement(24263..24559)
                     /locus_tag="VF226_08595"
     CDS             complement(24263..24559)
                     /locus_tag="VF226_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7074083.1"
                     /translation="MTPNASAVLQPTVTVILAPALLRLFPEAARRVDVEASTVGEVMD
                     ALDDRWPGMRDRLCDSTPAVRRHINVFVNGERASLDTHLESGAQVWVLTAISGG"
     gene            complement(24581..25666)
                     /locus_tag="VF226_08600"
     CDS             complement(24581..25666)
                     /locus_tag="VF226_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX7074084.1"
                     /translation="MAKKVLVLVGTKKGAFLLESDARRNSWELSGPFCETWPINHVSG
                     DPRTGTIYATGGNEWFGPAVWKSTDLGKSWTHSSQGLAYPEGEQPIKAGWSLALGSGG
                     RLYAGVEPAGLFYSEDEGQTWSHVSGLRDHPSRPEWQPGGGGLILHSIVLHPNDPDQL
                     WIAISSAGVFHTADGGKSWEPRNRGTRADYLPEDQRYPEFGQCVHCLAMAPGMPDRLY
                     QQNHCGMYRSDDGGRNWVSIEEGLPSTFGFPAAVHPRDPDTLYLLPLNGDIAGRFVPD
                     GKTAVWRTRDAGKTWQDLREGLPQKNAFLGVLRQAMATDPAEPAGVYFGTSSGALFAS
                     ADEGESWNCIALHLPTITSVETMVIDS"
     gene            25779..26225
                     /locus_tag="VF226_08605"
     CDS             25779..26225
                     /locus_tag="VF226_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7074085.1"
                     /translation="MSKPNELPFEITPVVRDACLCLHVQRAARAVARRYDEALRPLAL
                     TNGQFSLMMSLNRPEPPTMGQVSAVLAMDRTTVTANLKPLERRGLVEVQIDDHDRRSR
                     RLKLTQAGRKLLAKAMPIWKKTQAKNKTLLPDSDLEKLLLYLRAIS"
     gene            26428..27789
                     /locus_tag="VF226_08610"
     CDS             26428..27789
                     /locus_tag="VF226_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7074086.1"
                     /translation="MNLSFMLKERAAAGRPIRIGQVGAGKFGTMFLAQARTTTGMHVA
                     AVADLNIPRARSQLKMASWPEEQYGAGSLDEALKTGKTFLTEDAETMIRHPAIDVVVE
                     ATGDPRTGIRLCQLAIENGKHIIMVNVEADVVAGPLLARKARQAGVVYSLAWGDQPAL
                     VCEQVDWARACGFKVVAAGKGTRYLPSYHKLTPETVWNELQKYLKIDDPKSINLKMFN
                     SFLDGTKSGIEMSAICNATGLESQTDGLTFPPATRFEHADICKPKSEGGVLEKAGVTE
                     VTSMVYRDGSDVPHNLAMGTYVVFEAAEDNDYSRRCFKEYNLLPDKSGRYAALYRPIH
                     MIGLELGISIASAVLRKEPTGAPICFNSDVVATAKRNLTAGEILDGEGGFCVWGKQTP
                     ASISLKEGYLPLGLAHNVKLKRDIPEGQRLRWDDVAIDPDDAAVKARREMEAAFGRLN
                     EGP"
BASE COUNT         5352 a         8866 c         8674 g         4929 t
ORIGIN      
        1 ccatgcaatc cagaggaccc ctcaccgggg tgcgcgtgct cgagttcgga caaatcgcgg
       61 cggggccatt cgccgggtcg ctgctggccg atctcggtgc ggatgtcgtc aaggtcgaaa
      121 atcccgcggg cggcgatggc atgcgcggat ggccaccatt gacgaaatcc gaggacgggc
      181 gggtctacag cgaaaacttc gcctccgtga accgcaacaa gcgttcgatc gcggtggacc
      241 tgaagaacca gcgcgaggtc ttgcgcctga agcagctcgt ctcgaaggcc gacgtcatca
      301 tcgagaactt ccggcccggc gtgctcaagc gccttgggtt cggctatgag gacctgaagc
      361 cgctcaaccc gcggctcgtg tactgctcga tctcgggcta tggccagcag ggtccctacg
      421 ccgagaaggg agccttcgac gtcacggtcc agggcatgag cgggctgatg agcgtcaccg
      481 gcgaggaagg gcgcccgccc gtgaagtgcg gcgtgcccgt ggcagacttc tgcgccggcc
      541 tctatggtgc ctacacgatc accgcagcgt tgatgcaggc gcggcagacc ggcgaaggtg
      601 cttacatcga ctgctcgatg ctgggggcgc tcatcggcgt cgcggccttg cagacgagcg
      661 agtatttcgg aacgggcaag tcgggcgggc ggctcggctc ggctcatccg agaaatgccc
      721 cctatcaggc ctatcgcgcg agcgacgact acttcatcat cgcggcgggc aatgacgccc
      781 tctggcgcca ggtggcggag gcggtcggcc ttgacgacct tccgaacgac ccgcggttca
      841 gaacccaact cgaccgggcg cgcaatcagg cggcacttgc cgaaatcctc gcagagcggt
      901 tcaagacgca aacagccagg gaatggctcg aggaaatgga tcgtcgcggc gtgccatgcg
      961 ctccgatcaa tacatacgcg gaaatcttga gcgatcccca cgtgctaggc atggggctgg
     1021 tccgtcctct gacgctgccg aatggggtcg agaccaagac gaccgctttt ccggtcgcca
     1081 tctcaggcta cgcctttgag gtgcggcatg ccccgccaga gctcggcgcc catacagatg
     1141 aggtcatgac ggagtggcta ggcccgcagc tttcggaatt gacctgatcg gctccaggtt
     1201 cggggtgggc tcgcggttca gctcgccgtc accctgttcg tcggcacctt caccttgacg
     1261 ctctggttca agaggtccat caacacgacc agcctgtcct tttcgtccat ctcgatgatg
     1321 gtggcgacca acccgtcgaa cgggccgcct tgcacgcgaa cctgctggcc cggtctgaga
     1381 ggctcggccg gcttgacgat gacgccgtcc acctcacgcg ccttcaggcc atcgatcagc
     1441 ccgtcctcga caaagctgag ctgatcgcca aagcgcacaa gcgagcgcac gccgaacgtc
     1501 gacagaatcg ggcgccagcg cccggcaaga tctatgcgag cgaacagata gcccggaaac
     1561 agcggccgcc gcacatccct ggtccgccgc gcatggcgta cctggcgctc gaccatcgga
     1621 caatatgcat cgaagccctg gttcgtcagg ttttcgagcg cgatgtgctc ccggtgcgga
     1681 tgcgtgttga cggccaccca ccgccggacg gctccggcgc cattcgcgat cgcaggctcg
     1741 cccagggcat gtgcgctcgc aagttcctct ccgccggtaa agtctccacg catccccacc
     1801 cccgcccttc ggagtacttg cgacggatca actgtagtca gcctgccgag cttgtccata
     1861 gcgccggaat ccgcgggagc ggcctgggga tcacagagcg cgctggaacc tacctgtttc
     1921 tgttccgttc ggatttctga cgtgaggtct tcggggtgct cgcgaggttg cacatactga
     1981 gatgccggct accgctcagc tgacgcttgc gcttgaggat ttcgacgctg ccgttttcga
     2041 cctcgacggg gtcgtgacga aaacggccgc cgtgcacgcc gcagcctgga aagagctgtt
     2101 cgacacctat ctgaaggcgc gcgcggatcg caccggcgag ccgttccggc cgttcgacat
     2161 tcagcgggat tatctccagt acgtcgatgg caagccgcgc tacgaaggcg tgcgcagctt
     2221 tctggcatca cggagtatat ctcttccata cggcagtccc gaggactccc ctgatcagga
     2281 aaccatctgc ggcctcggca atcgcaaaaa cgagatcttc aacgcccagc tcactcgcac
     2341 tggcgccgag gtataccaga gctccttaca cctcattcag gagctgagaa ggcacgggat
     2401 caaaacggca ctcgtctcct cgagcaaaaa tgccaaggcg gttctagagt cagcgcggtt
     2461 gacggagtta ttcgacgcct gtgtcgacgg catcgaggcg gcccggctct cgctcagagg
     2521 caagcccgac ccggacatct atctccatgc cgtgcgtttg ctcgacgtaa agcccgcgcg
     2581 agccatcggc gtcgaagacg ctttgtcggg cgtggaagcc atacgagcgg cgggttacgg
     2641 gctcgtcata ggagtcgatc gcggcgatca ggctgacgct ttgcgcgatc acggcgcaga
     2701 catcgtcgtg cgcgacctgg cggagttgca gctagtcgag catttatagt tttctggccg
     2761 ggaacccgat cagctccgac atgtctggtc gatcggaagc tgtccgcttt cagggaaaaa
     2821 ataccaatcg cattccattt ggagagaagg cgccgtggcc gcaaattctg atccttatgt
     2881 ccgatggctc gaagatctcg gctctgacga cgttggccag gtcgggggta agaacgccag
     2941 cctcggcgag atgatccgca gcctcaagca agaaggcatc cgcgtgccgg acggcttcgc
     3001 gacgaccgcg tacgcgtatc gcgaattcat cgccgcaaat cacctggaag acgccattcg
     3061 cgaccgcctc gaggcgctga aagccggtcg tgcaacgctc cgcgagaccg ggcaggcgat
     3121 ccgccgcctg tttctcgaga gcgaattccc cgagccgatc gcccaagcca ttcgcgaatg
     3181 ctaccgcaag ctcgcagaac gcagcgggct ggacgatgtg gccgtcgccg tccgctcgag
     3241 cgcgacagcg gaggacctgc ccggtgcgag cttcgcaggc caacaggaaa cattcctgaa
     3301 tgtccgcgga gagcgggccc tgctcgacgc ctgcagacgc tgctatgcat cgcttttcac
     3361 cgaccgcgcg atcagctacc gcgatgagat gggtttcgac cacatgcagg tcgcgctctc
     3421 ggccggtgtc cagcggatgg tgcgatcgga tctcgccggc tccggcgtga tgttcacgat
     3481 cgataccgag acgggattcc cgaacgtgat catcatcaac gcagcctggg gtctcggtga
     3541 aaacgtcgtt cagggcactg tcaaccctga caaatacgcc atcttcaagc cgccactcag
     3601 agatgagcgc tatcgtccga tcatcgagaa aacgatgggc acgaaagaaa agaaaatgat
     3661 ttacgccgaa ggcggcagtg gcaggacgaa aaacgtcaat accacccgca gggagcgaga
     3721 ggccttcgtg ttgagcgata gcgatattct gcaactcgcg cgttgggccg ttctcatcga
     3781 gaagcattat ggccgtccga tggacatcga gtgggccaag gacggcgaaa cgggcgagct
     3841 cgcgatcgtt caggcacggc cagaaacggt gcaggccgct gccagatcgg caccgctgaa
     3901 gatctatcgc ctgaagggaa aacagccaaa gccggtcatt tctggtgcag ccatcgggca
     3961 ggcgatcgca gcgggcccag tctgcatgat ccggtcggct gaagaaattt ctgaattccg
     4021 gccaaactcc attctggtca ccgaaaccac cgaccccgac tgggtcccga tcatgcgcca
     4081 ggcggcaggc atcgttaccg accatggcgg cacgaccagc catgccgcca tcgtcagtcg
     4141 cgagctgggc gtcccggcga tcgtcggaac gggacaagcc acacgcgtgc tccgcgacgg
     4201 ccaggacgtg acgatctcct gtgcccaagg cgaaatcggt tatgtctacg acggcatcct
     4261 cgatttcgag acgcaagagg tcaatccgga ggcattacct gagccgcgca cggcgatgat
     4321 gatcaatctc gccagtccgg ccactgcatt tcagttctgg cggctgccga cgaaaggcgt
     4381 gggattggca cggatcgaat tcattatcag caactacatc aagatccatc caatggcgct
     4441 ggttcatccc gatcgcgtgg aagatcccga ggcacggcac cagatcgaac agctcacccg
     4501 cggatacggc gacccgcgcg attatttcat cgatcagctc gcccgcggca ttgccaagat
     4561 cgcggcacct taccatcccc accccgttat cgtccgcctc agcgacttca agacgaacga
     4621 atacgctcat ctgatcggcg gtcagtcatt cgagccgagg gaagaaaatc cgatgctcgg
     4681 attccgcggt gcatcgcgct actacagcga ccgttatcgg gatggtttct ccctggaatg
     4741 ccgcgccttg cgaaaggtgc gcgaggacat cgggatcacc aacgtcgtcg tcatggtgcc
     4801 gttttgccga accccggccg aagctgatcg cgtgctggcg atcatggctg agcaggggct
     4861 ggaacgtggc aaggagggcc tgcagatcta catgatgtgc gaaattcctt cgaacgtcat
     4921 tcttgccgag gaatttgcaa agcggttcga tggcttttcg atcggcagta acgatctgac
     4981 ccagctcatc ctgggggtcg accgcgattc ggccgagttg gcgcctttgt tcgatgaacg
     5041 caacgaggcg gtgaagcgag ctgtaagtga agccatcaag aaagcgcaca aggccggcat
     5101 caaggtcggc atctgcggcc aggcgccgag caattatccc gaattcgcgg cctttctcgt
     5161 cgagcaggga atcgattcca tatcgctcaa cccggacagc ttcatcgcga ccgtgaccca
     5221 tgttgcagag gtagagcggc gcctcgcctc atctgggatc cgctaagccc accgcgcgtt
     5281 ccggtcgcag gccgatgggt gagaagagga gcagccgccg tgaatgggaa cgacaagatc
     5341 cgggtcgccg tgaacggcta cggcgtcatc ggaaagcgcg ttgcggcagc ggtcgcacag
     5401 caggacgaca tggagctcgt cgggatctcg gacgtggtgt cggactggcg cgctcgtgcc
     5461 gcaatcatta ggggcttcaa acttttcggc gcgaccgccc aaagcgccga ggccatgaag
     5521 caggcgaagc tcgacgtctc cggcacgctc gacgatctgc tcgggaccgc cgagatcgtg
     5581 gtcgactgca cgccgaaaaa gatcgctgcg acgaacgtcg agcgctatcg cgagcggggc
     5641 gtcaaattcg tcctgcaggg tggcgagaag caccaggtca cgggccactc gttcgttgcc
     5701 gaatcgaact tcgccagtgc ggtcggacgg gaatcaacgc gcgtcgtatc gtgcaacacc
     5761 acgtccatcg tgcgcaccct tgccgcgctg aagcgcgcgg gcctgctgaa gaaggcgcgc
     5821 ggtacgctgc tgcggcgcgc aaccgatccc tgggagagcc accagagcgg cattatgaac
     5881 acgctggtcc ctgagccgga cattcccagc catcaggcac cggacgcaca aagcgtggat
     5941 cccgagctcg atgtcgtcac gatggccgtc aaggtgccgg aaacgctggc gcatatgcat
     6001 tactgggctg tccaattgac gcgcccggcg agcaaggacg aggtgctgga agcgttccgc
     6061 acgtcttcgc gcatcgcctt catccgcatg cgcgataatc tcgttgcgat aaatgcggtc
     6121 aaggaaatga tggccgatct cggccggccc cacgacaacc tgtacgaggt tgccctttgg
     6181 gaggatatgc tcaaggtcca ggacgatgag ctgttctatg cctatatggt agacaaccag
     6241 gccatcgtca ttcctgagac gatcgacgcc atccgcgccc tttcaggcag ggagaaggat
     6301 tggcaggcct cggtcaagaa gaccaatgcc agcctgggga tcggcttgtt gaactggtga
     6361 ggagaaccgc tgacgcatgc ccgcgatttg cacgctgacc ctgaaccctg cgctcgacat
     6421 cgcgaccgca acggatcggg ttacggccac gcacaagctg cgctgcacca cagcacgcta
     6481 tgaccctggc ggcggcggga taaatgtggc gcgtgtgatc ttcgagctgg gcggcaatgc
     6541 gactgcggtc tacacggcag gtggtccagt ggggcactcc ctgcaggaac tgctcgatgc
     6601 tgcaggaata ccgcaggtcg ttattccgat ccggggcgaa acgcgcgtca acatcacggt
     6661 cgacgagcgg gtcagcaata accaattccg cttcgtcttc cccggcccgg agctgtcccc
     6721 cgaggagcag gagcagtgca tcgaggctgt cgcgaagctc gatccaccac cggaatttct
     6781 ggtggcgagc ggcagtctgc cgcccggcgt tcccgccgac ttctatgcgc gggtcgctcg
     6841 gctggcgaag tccctaggcg ccaagtactt tctcgacacg tcgggtgatg ccttgaggga
     6901 agcggggcac gagggcattt atctcgtcaa accgaacctc agggagctct ccgagttcgc
     6961 aggccgcgat ctcgcatcga ggagcgatca gatcgatgcc gcacgcgagt tgatcgccga
     7021 aggccgcgcc gaggtcgtcg tcctgtcgct tggcgcggac ggcgcgctgc ttgtgaccaa
     7081 tgagctcgcg gagcacttcc cggcgctcca ggttccactc cgcagtgctg tgggcgccgg
     7141 cgacagtatg ctgggcggca tcgtgtattc acttgcccgt ggcaatccgc tccgcgacgc
     7201 cgtgcgctac ggaattgccg ctggagcagc ggcgctcatg accgagggaa ccgagctcaa
     7261 ccgtcgcgaa gacacggaac ggctttacgc tgaaatgctc tccgaggcgg gctgaatcga
     7321 aaaggagctg gcgtcaggca tcgctcgaag cgcagtcgtg ggcgcgcacg acgacaaggc
     7381 ggttgaccac atccttgacc ccgggcacct gccgggcagc cgagacgagc ttttcaagca
     7441 ggctcgcatc cggcagggcg ccccgcagct ccaccgttcc gttctggacc agcacattga
     7501 tagaagtgcg gcttccgcgt gcttcaccgg acagagcctg aagcacttgg tccctcaatg
     7561 cataatcctc ggcggtggca tcgggcatca tcttcgcctc caatgccaat ccgcgcagga
     7621 gatccgccct gctgacgatc ccgacgacct tgttgtcccg cacgatgggg acgcgcttga
     7681 tgccgttggc cttcatcagc ttgacgatgt cctgaaccgg cgtctcctcc gtggccgtaa
     7741 tcacctgccg tgtcatcgcc tctccgacgg tctgcgcgcg aggcgatatc gccgcgagcg
     7801 ccggcttctc aggatcgaga atgaactcca gccagcggcg gcgctggccg tcgcccgtga
     7861 tacctgtcag caggtcgcct tcggtaatga tgcccaccag atttccgccc tggtccacga
     7921 ccggcaggcc gctgatgcgg taatcgagca tcatgcgagc cgcctcccgg atcgaggcat
     7981 ccggctgaat ggttgccgca ccaaaggtca tgacctgagc cgcttgcatt ctcgcctccc
     8041 tgagttcatg tcacaaacgc gcggacgggc cagggcacac atgccagcct acagaaaaat
     8101 gggagcggga cctcgatcca gcaataaggt tccgctcggt tggagaacgc ccggcagctg
     8161 gaggaggaga tgatcactgt ggagcgagtg agcgacgccc cgctcacgtt cgaggtcagc
     8221 gtccgcgaag gcggaagcga aacccgtcac cgcgtgacca tggccgatgg catggtcgcg
     8281 cagctcgggc aaggccaatt cacgccggag cagtgcatcg aggcctcatt ccggttcctg
     8341 ctcgaccgcg aaccgaaaga atcgatcctc cgggagttcg acgtggccgt catcgcgcgc
     8401 tatttcccgg agtacgaggc ggagctgcca cgctatcttt ccgaccgata gggagcggtc
     8461 cacgagatgc caacccgaca gagacccagc ctcagctcgg cggcatgatg tggatgcctt
     8521 cggcgagcag cgacacgctc gccgccacgc caggctcgac gccatttcgg cgcgccacat
     8581 gaagaggcac cgaaaagtga atcggaaggt tccgatcgtg attgggtcgt agcgtcaggt
     8641 gcgctgtctg gccgatcggc aagaccgcct cgatgatccc cggcaccggg ttctcgtgct
     8701 caccccggga cgggcggtcg cggcggtgga ggacgacgaa gccatcggga atgacccagg
     8761 tgattggctg gccgggttgc aggtccggcc gcgccggcgc ctcgatctcg aggccagccc
     8821 atgcgatgat cgaaggctta ccggctgtgc ccccggttac gatccggcct tcgaagatgt
     8881 tgcggaggtc gacgaggcgc gcgacctcgg cagaaacagg cctcgtggtg atctccgccg
     8941 gggtccccgt ctgtagtgtg cggccgcgat gcagcaccgc catgcggtcc gcgagcataa
     9001 gcgcttcgtc gagatcatgg gtgacgagca cgacaggcat gttgagcccg cgtcgcagct
     9061 cggcgatttc gcgatagagc ctttggcggg tggccttgtc gaccgccgag aacggctcgt
     9121 cgagcagcag caccttcggc tcacgcgcca gtgcccgcgc caccgcgacc cgctgctgct
     9181 ggcctccgga tagctcggcc gggcggcgct gctcgaggcc ggcgagatgg acctgcgcca
     9241 ggagtcctct tgccttcgcc gcccgctcct tggccggcac atgggtcatt gcagccatga
     9301 tattgccgag cgccgtcaga tgcgggaaaa gggcataggt ctggaagacc atcccgaccg
     9361 cccgctgatg cgccggaacg aagcggcccg cagctgccga aaaccaggcc tccccgttca
     9421 cttcgatctt accgagctgc gggcggtagg ttcctgcgat cgcgcggaga atcgtcgact
     9481 ttccgctgcc cgacggtccc acgagcgcca gcacctcgcc cggggcgcag gagaattgcg
     9541 ccgcaagcgg tatcggccgc tcctgactga gctcgactcg cagtccctcc acggcgctca
     9601 tcccaatcgc cggccgaccc gccgcgaaaa ggcatacgtg agcgcaatcg tggcaagcga
     9661 aatggacagc agaagagccg acatccgccc tgcggccatg tcatcgaacg cctgtacgcg
     9721 gtcgtagatg gcaatggcga tcgtcttggt ttcgccgggg atcgagccac ccaccatgag
     9781 cacgatgcca aactcgccga gcgtatgggc aaaggagagc accatggccg tcaggatacc
     9841 gggccaggcg agcggcagct cgacctttct caacgcctgc atcggcgaga gcccgcagca
     9901 ggctgccgcc tcgcgcacat cggacggaat cgcctcgaag ccacgctgca tcggctgaat
     9961 ggcaaatggc agattgaaaa tgaccgacgc gatgagcagg ccctcgaagg tgaagacaag
    10021 ctgacctccg aaaagcgcct gccacaattg gcccatcggg gaagccgcgc caaaggcgac
    10081 cagcagatag tagccgagaa cggtcggcgg caggacgagc gggagggcta cgatcgcctc
    10141 gaccagcccc tttcccggga actggcggta ggccaaggcc cgcccggcga aaatgccgat
    10201 cggcagcagc agaaggatcg taaagaatgc caagcgtagc gagaggaaaa gcgcggtcca
    10261 gtccattctc agtcctctcc gcgcgcgatc agctcgcctc gccaggcaat acgaatccgt
    10321 attttcggaa cacgcggcgc gcctgctcct cctgcagata ggcatagaag cgttccgcga
    10381 cggggccagc cgtcttcaga agcaccatgc ggttcctgag cggcgtgtgc cactgctccg
    10441 gtatcagcgc gaacgtgccg agctggctga ggtgcggtga cacggcaaga gagtacgcga
    10501 tgatgccgcc ctgggcgttt gccgatgtgg cgaattgtgc cgcctgcgaa atgttttccc
    10561 cgaacacgag ctttggccga atgcgatccc ataactgctt atgccgcagc gcctcctctg
    10621 cccgtttgcc gtaaggcgcg tgctccggat tggctatggc gaagcgcttg atgcggccat
    10681 cgtcgagtgc tgccgcaagg tcatccagcg aagcgtccgg cttcagcagt gatccatgag
    10741 ggacatagat cacgatccgg ccgacagcgt agatcgctcc ctcatcgcgc gtgagcccgg
    10801 accgcgcaag atcaagcacg tagtcttcat cggccgatag gaacatctca tagggggcgc
    10861 cctgccggat ctggcgcgca aagtttcccg aagagccaaa cgacaatcgg accgattggc
    10921 ccgtctcgga cttgaagccc tcggcgattt cctccagcgc gaattgaagg ctcgcggcag
    10981 cggcaatgat ggggacattg gcgcttccgt tctgcgccgt agcgcctccg accggcgagg
    11041 caccaaacgc gacgagcgca agggaaaggc ccagcgcgac gcggcggctc agcaaattca
    11101 tcagcttcac ccctcgaagt gcagttggaa catatcggga cgtccgggcc accacaatcg
    11161 caatcggccg atctctcgtg tgacgaaggg ctctccctcg gatcattgaa gtgcgatagt
    11221 gaaagatgta tccatgatgc cgcttcaagg aggcctgcac aggtgacatt ctcgccgcat
    11281 cccaaagcgc cgggcctgga ggtcggcgtc accgagccga tgatccgcga gctcgtgcac
    11341 accttctatg cccgcgtccg gcgggacgag ttgcttggcc ccatcttcaa cactcatgtc
    11401 gcagactggc cggaacacct ggacaggctc tgcgctttct ggtcctccgt cacgctgatg
    11461 acgggccggt tcaagggcaa gccagtgccg gtccatgcca agctgcccgg catcacggtc
    11521 ccgcatttcg ctcgctggct ggcgctcttc gccaggacgg cgcgcgaagt ctgcccgccc
    11581 gaggctgcgg agctgttcat cgaccggtcc gagcggatcg cgcaatccct tcagcatgcg
    11641 atcgcgattt gccgcggcga gccgctcgag gtgcatcatg atgccgcctg aggtggccga
    11701 gtggcggaag gctcagcgcg accggctcct cgaaacgcgc cgcaacatgc ctctgtcgga
    11761 acgggccgcg ctcatcgcgc cattgctcgc caacttgcgt gacctgctgg agacgttgtc
    11821 cttcaactgc ctcggcatct actggcccat caagcgcgaa atcgacgtgc gtccgctggg
    11881 tgatgagatt tgccgatcgc ggggcgcaac gctggccctc ccggtcgttg tcgagagggg
    11941 cgcgccgctc gagtactgga agtggcagat gggcgatgcg gtcgagcgcg gattctggaa
    12001 catccccgtt ccaaaacagc gccgccctgt agagcccgat accgtcatcg ccccgctcgt
    12061 cgggtttcac gatcattacc gcctcggcta cggcggcggc tatttcgacc ggacgctggc
    12121 caagcttcgc ccgttcgcca tcggcatcgg tttcgagttc agtcgggtcg acggatttgt
    12181 tccgcagcca cacgacattg ctatgaatgt catcgtcacc gagagcgcca tctatcagaa
    12241 gtgatcgttc ggagggggac agagatgctc gggatttcga acttccacga ggtgttcctc
    12301 tccacgatgc ccgacggtgt tctgctggcg gacaaggacg gcgtgatccg catgtggaac
    12361 gccggctgcg agcggatctt cggcttcacg gcagaggagg caatcgggca gccgctcgac
    12421 atcatcattc cggaaaacct gcgtgcccgg cacggcgaag gctacgcgca aaccatgcgc
    12481 accgggaaaa cccggtatgg gcaaggcgat cttctctccg tccccgcatt gcacaagaac
    12541 ggaacccgca tttccgtcga gttttcgata ctgccgttcc gcgatgctga agggcgaatg
    12601 gtcgggatcg gagcggtttt gcgagatgtc acacgcaaat ttgaagagat gaaagcgctg
    12661 aagaaggcgc tggccgcagc ccgcgccggc tgaaaccagc agaagattgg agcaacccat
    12721 gtggccagac cgcaggatca ccgatctgtt cggcgttgag catcccatcc ttctggcccc
    12781 catggctggc cccagcacgc cggagctcgg catcgccgta tcggaagcag gtgggctcgg
    12841 ctgctatgcc tgcgcactct cgacgcccga gcagatccgc accgcgatga gcgtcatccg
    12901 gcaacggacc gacaagccgg tcaacctcaa cttcttcacg catacccagc cagtgccgga
    12961 tgccgatcgg gaggcccggt ggaaggcgcg gcttagtcct tattactcag agctcggcat
    13021 cgatccaaac gcacctaccc ccactgcaag cagagcgccg ttcgacgaca ccgcttgcca
    13081 gatcgtcgag gagttccggc cggaggtcgt tagcttccat ttcggactgc cggaggacaa
    13141 gcttctgcag cgggtgaagg cgacgggcgc gaaggtcatc gcgtcggcga ccaccgcccg
    13201 ggaagcgaaa tggctcgccg agcgcggctg cgacgccatc atcgcccagg gcgccgaggc
    13261 gggcggacat cggggcatgt tcctgacgga caacatcgcc tcacaggcgg gcactctcgc
    13321 gctcgtaccg caggtcgtcg atgcggtcga tgtgccggtg atcgcggccg gcggcattgc
    13381 cgacgggcga ggcatagccg ccgcgttcgc gctcggcgca gcgggtgttc agatcggcac
    13441 ggcctatctc ttctgccccg aggcgaaggt gtcggccatg catcgggaag cactgcgctc
    13501 ggcccacgat gacgacacgg tgctgacgaa cgtctttacc ggccggccgg caaggggcct
    13561 gatcaatcgc atcatccgtg aagtcggccc ggtctcgccg gacgcgcctg cctttccgct
    13621 cgcagccggc gcacttgccc cgatccgggc cgctgccgaa gccaaaggct catcagactt
    13681 tacgtcgctc tggtccggac agtcggccgc cctcggccgc gaaatgcctg ccgcggaatt
    13741 gacgcgcaag ctggcacgag aggcgctgga ccgcatgaag gcgctcagcg gaacctgatc
    13801 cgacccccgc gcgctctctc cgaaatgtga gccgcgcaac cgtgacgggt agtttcggtt
    13861 ggactaaggt tcaccaagag tttgcccaag gagccggtca tgcttcgcat tctggcggcg
    13921 ctcgcagccc tgaccttcct gcaacttccc gcgtgggccg aatactcgga acaatacttt
    13981 gatgtgccca agggggacgg gccccacgac gtcgccgtcg ctccggatgg aactgtctgg
    14041 tacgcggggc agaaagccgg cgtcgccgga aagctcgacc cggcaacggg caaaatcgag
    14101 cgcatcaccc tcggccagaa ttcggcacct catggggtga tcgtggggcc tgacggtgcg
    14161 ccgtggttca ccgacggcgg gcagaatgcg atcgtgcgcg tcgatcctga gacacacagc
    14221 gtgaagatct ggccgttgcc gccggaacga atgccctaca cgaaccttaa tacggccgct
    14281 ttcgacggga aggggcgcat ctggttcacc gggcagaacg gcatctatgg ccggctcgac
    14341 cccgagacgg gcgagatgaa ggtctgggat gccccacgcg ggcgcggacc ctatggcatc
    14401 acggccactc ccggcggcga gatctggttc gtgtcgctgg ccggcagtta tctcgccaac
    14461 gtcgacttgg agaccggcca ggccgccatc tacgagccgc cgacgcccaa tcagggtgcc
    14521 cggcgcgtct ggtcggattc gcaggggcga ctctggatca gcgaatggaa cacgggcaac
    14581 gtcagcgtct acgatcccgc ctcggatacc tggcgacagt ggaagctccc cggcgagaga
    14641 ccgcgcactt attccgtctg ggtcgatccc gacgacaagg tctggctgac ggaatggtca
    14701 agtaacgccg tggttcgctt cgacccggag acagaaaagt tcgagagttt tccaagcaat
    14761 cgggcgaatg ccaatgtgcg ccagatgctt gggcgcaagg gcgaagcctg ggctccggaa
    14821 tccggcacgg accggctgcg tgtcatccgt tatgcttcca agacggactg atcatcggtg
    14881 ccgcgccccg aaatcgctgc tctcctgatc gtcgtgacat cggtatcggc cctgccgtcc
    14941 gtcgcaatgg cagcggaacc cggagatccg gtccgtggtg agcgcgtttt ccagcgctgc
    15001 tatgcctgcc actccgacga accagacgag ggcaacttgc aggggccgaa cctgtacaag
    15061 gtccttggcc ggccggcagc cgtggtcccc ggcttcgaat attcccaggc tatgaaacag
    15121 aaggcggcag caggcctcgt gtgggatgct gccacgctcg acgctttcat cgccgatccc
    15181 gatgcggtgg tgcctggcac gctgatgagc attcctccgg ttcgcaacgc tcaagaacgc
    15241 agcgacctca tcgcctatct cgcccgcttc ggcaggtttc aggattaagg ttgccggtgg
    15301 aaccaatcga gagccgcggc tgttgagggc ctgaaacccc aaacgccagc ggagaacgac
    15361 cttgcagccc accactcagg agcggacgcg ggccgagctc gagcctcaac gggacgtctc
    15421 gatcagggta ccgctccgtc tcacgatcaa tggaacgctc tacgacctcg acgtaccgcc
    15481 ctggaccacg ttgctcgatc tcctgcggga ccggctggat ctcaccggcg ccaagaaagg
    15541 ctgcgaccac ggtcagtgcg gggcctgcac cgttctcgtc gacggtcgcc gtatcctgag
    15601 ctgcatgacg ctcgctgtga tgcaggatgg gcggcaggtg acgacgatcg aagggctcgc
    15661 cagcgacggc accctgcacc cgatgcagca ggccttcatc gaccacgacg ctttccagtg
    15721 cggttactgc acaccgggcc agatctgctc cgccgttggg ctgatcaatg agggacacgc
    15781 gcacacgccc gaggaagtgc gcgagctgat gagtggcaac atatgccgct gcggcgccta
    15841 ccccaacatc gtcgatgcgg tgatggacgt gatggcgaag gaaggaggcg gcgcgtgaga
    15901 ccgttcagct atacgcgtgc cggtgacgtc tccgaggcgg tgagcgaggc atcggcaggc
    15961 gcggtcaagt tcatcgccgg cggcaccaac ctcctcgacc tgatgaaaga ggacgtcgag
    16021 cggcctatgc ggcttgtcga catcaaccag cttccgctca accggatcga ggatacgccc
    16081 gatggcggcc tgcgctttgg cgctctcgtc cgcaacgccg atgcggccca tgatcctcgg
    16141 atcagagacc gctatccgct cctgtccagt gcattgctcg ccggagccag cccgcaactg
    16201 cgcaacgcag caacaacggg cggcaatatc atgcagcgta cgcggtgcta ctacttctac
    16261 gataccgcaa ccgcctgcaa taagcgaaag ccgggcagcg gctgtggcgc aatcggcggc
    16321 atcaatcgcc ttcacgcaat tctcggcgcc agcgaccatt gtatcgcgac acatccgtcg
    16381 gacatgtgcg tcgcgcttgc cgcactgaac gccgaagtgc gggtcgccac gcccgatggc
    16441 ggtgaacgcg tgattccgtt tgccgacttc caccggctgc ccggcgacac gccgcatatc
    16501 gagacgaatt tgcggcccgg cgaattgatc acggccgtcg atctgcccaa cgagatgttc
    16561 aacaagcact acacctacct gaagatccgc gaccggctct cctacgcctt tgcgctggtc
    16621 tcggtggcgg ctgcgttgca catcgaaaat ggccggatta ccgatgcccg cctcgccctc
    16681 ggcggcgtcg cgcacaagcc gtggcgcgat aacgacgccg aacggttgct gattggcaaa
    16741 tcgccggaac gccaacactt ccaagccgtg gcggatgcca tcctgcggga cgccaaaggc
    16801 caaggcgaca acgacttcaa gatcccgctc gcccgcagag ccatcgtgcg ggcgctcgag
    16861 caggccgcga acggcacccc gcaatcgcaa atcgacaagc acgtcgcctg aggaggcgag
    16921 ccatggcgac taccgttcac atcggcgaac cgctgaaccg ggtcgatgga gtggcgaagg
    16981 tgacgggcgc ggcgcggtac gcggcagagt atcccgtcga ggggttgctt tacggctttg
    17041 ccatctccag ccccattgcc aagggccgca tcaccgcgat cgacacgtcc gcggccctgg
    17101 ccgtgccggg catcgtcgag gtgatcacgc acctcaatcg cccgcacgtc gcctggcagg
    17161 acataagcta tcgagacgac gcctctccgc caggctcacc cctgcggcca ctctacgatg
    17221 aaaaaatcta cttcagcggt cagcccattg cactcgtcgt ggccgaaacc ccggagatcg
    17281 cccgctacgg cgccagcctg gtagccgtca cctacgacgc cgagccccac aacaccgatt
    17341 tcgacaccgg attgtccgag cgttacccgc ccaagaaggt acgctctcac gccgttcccc
    17401 cggatgacag gggagatgcc gacggcgcat tgaaatccgc cccggtgcgc atcgacggcg
    17461 actaccgctt gatgatggaa gttcacaatc cgatcgagat gcacgcttcc accgcgatct
    17521 gggaggatgg cggcaagatc acggtttatg acaagaacca gggctcacag aacgtccaga
    17581 cctacctttc caacgtgttc gacctgccgg ccgacaaggt ccgcgtgctc aatccgtatg
    17641 tcgggggtgc cttcggatcg gggctcaggc cgcaatggca ggtcaacctt gcggtccttg
    17701 ccgccaagat gctcgagcgc tcggtccgcg tcgtcatgac ccggcagcag atgttcacgc
    17761 atacgcatcg ccctgagtgc ctcatgtgcg tgtcgctcgg agcggatgag aacggcaagt
    17821 tgtcggcaat cgcggtcgat gccacgagcg ccacctcgcg atacgaggac tactcggaaa
    17881 gcatcgtgaa gtggtcaggc attatctacg gctgcgaaaa cggttctttc agtcacgagc
    17941 tcgtcccgct cgacacgtcg acaccgggcg acatgcgggc cccgggcgca gcgacgggca
    18001 tgaacctgtt cgagatcgcg atggacgagc tggcgtacgc ggccggcatg gacccgctcc
    18061 ccctgcgcat cgccaacttc actgacttcg atctcgtgac gaatgcgccc tataccagca
    18121 aggcgctgct ggcggcctac cgggaaggcg cggcgcggtt cggctgggac aagcgcagcc
    18181 atgcgccgcg ctcgatgcgg gacggaaagg agctcgtcgg ctgggggatg gcgagcggca
    18241 tctgggaaac cctgctggcg gagatcgccg cccgcgcgac gctttcggcc gacggcagcc
    18301 tggagatcgc gtccgcggca tctgacatcg gcaccggcac ctacaccatc atggcccagg
    18361 ttgcaggcga aacgctcggc cttccagcca accgcattcg cgtgaagctc ggcgattccc
    18421 atctgccgaa gtcacccctc gaaggcggct cgtggatggc tgcctccgtc ggcgccgctg
    18481 tccagctggc ctgccggtcc ttggcgggca agatccatca gatcgcggcc aatatgaaca
    18541 acccggcctt cggcaccccg acgctggacc aggtcgcgtt cgcagatggg gccatccagc
    18601 tcaaaggcga cccgacgcgc gggatgaggt acgaggacgt cctgaaggca tcggggcagg
    18661 cttcgatctc agccgaggag acctacaaac cccaccggcg gcgcgacaag acgagcaacg
    18721 cccattcggc ggtgttcgca gaggtgaagg tcgacgagga gcttggggtc gttcgcgtca
    18781 cccgcatcgt ctgcgcggct gccgccggcc gcatcctcaa tcccaagacg gcgcgcagcc
    18841 agatcctcgg cggcatcgtt atggggcaag gcatggcgct cgaggaagag acgttgacgg
    18901 accatcggat cgggcgcatc atgaatcaca acctcgccga gtaccacgcg cctgtgcacg
    18961 cagacgttgg tcacatagat gtgatcttcg tcgatgaacc cgatccggag gtctcccccc
    19021 tcggcgccaa gggtctcgga gagctgggca tcgtcggcac tgcggccgcg atcgccaatg
    19081 cgatttacca cgccacgggg aagcggatcc gcgacctgcc gatcacgatc gacaagctca
    19141 tcaactgatc gaagccgaaa attcgtcctt ttcaagcacg aatagtgctg cccctcgtgc
    19201 cagtccggtc agtcccggct cgaggcgacg gcccgaactg ccgctgtcgc cagcacaaaa
    19261 cggccgtgct aaaataacta cagttgcggc attatgacct gacggctcag cggctcgtca
    19321 ctcaatccga gggataagtg cggccgggca agaacttcgg cctgtgatgg gccgcattga
    19381 acgtatactg tcgcgacggg ttccgggcgg tccattcgtt tcgcatgagc gcgtgctccg
    19441 gactgggtgg ggcaacgata aacagaagac gccgtcgaag ggtgaggacg acgtagatgc
    19501 gtaagctgct gctggccatc ctggcgctgg tcatagccgc cggcggggct gttttgctgg
    19561 tgcccggctg gcgggcgaaa ctgcacgccg agaagagccc caagccagct cagcaggccc
    19621 acgccgtttc cgtcgaggtc gcacccgcga tttcggccgt ctaccaggaa gatctgcgct
    19681 ccgtcggcac actgatgtcc gacgaatccg tccaggttgc atcagaaatc gccggccgga
    19741 tcgccagctt cgagttcaag gaaggccagc cggtctccgc aggcgatccg ctcatcaaac
    19801 tcgatgattc acttgcccag gccgagctcg tggatgccaa ggcccgattc gagctggcgc
    19861 gtgccaaccg tgaacgtgcg cgcaagctct ccaagagcgg atacacgacg gaaaaagcac
    19921 acgacgaggc cgatgccgcc ttcgagacgg cccgcgccgc tctcgagctc gcaaccgtca
    19981 agctggacaa gcacaccatc cgggcaccgt tcgacggtat catcggtctc aggaatgcct
    20041 ccgtcggagc ctacgtcagc atcggcaccc cgatcgtcaa tctggaaaag atcgacgtcc
    20101 tgaagctgga cttcaagctg ccggaagcgg ccctcacgca gatcacgacc ggacagcgtg
    20161 ttctggtgga ggtcgatgcc gtcccgaatc ggacgttccc agccgagatc tatgccatca
    20221 gccccatgct cgatgtgaat ggccgcgcac tgcacgtcag agcgcgcatg aagaaccccg
    20281 acctgttgct gcggccgggg ctgttcgcac gcgttacggt caaaggccgg gagacacgcg
    20341 aagtagtctt ggtgccggaa agcgccatcg ttccgcgcgg cggtgaaacg ttcgtctacg
    20401 tcatcgagaa cggcaaggcg atcgaaacca gggtgacact tggcaagcgc ggggacggca
    20461 gggttcagat cctctccgga ttggatgctg attccacagt cgtcgttgcc ggacagctca
    20521 agctgcgcaa cggtgtggag gtcgaggtcg tccagtcgca gaccacgcgc cagctggaga
    20581 agagctgatg aatcccgtcg aggtctgcat tcgcaggccc gtcttctcga cggtcctgag
    20641 cctcgttctg gtcctgctcg gtctcgtttc ctactcccga ctgaccgtcc gcgaataccc
    20701 gaacgtggac gagccgacgg tttccgtcac gaccacctac cccggtgcgt cgccgaacat
    20761 cgtcgagacc caggtcaccc aggtgctcga aggctcgatt gccggaatcg caggcatcga
    20821 cgtcctggag tcgaccagcc gatccgaaac gagccgcatc accgttcgct tccggcagga
    20881 cgtcaacccc gacgaagcgg cgaacgacgt ccgtgatcgc gtgagccgtg tccgcggccg
    20941 cctgcccgac gagatcgacg agccgatcat ccgcaaggtc gaggcggatg cccaagccgt
    21001 catgtacatc gtgttcacca gcgatcagat gtcggcgctg gagatcacgg actatgtcga
    21061 cagatacgtg gtcgaccggc tgaaaaacct caccggcgtc gccgacgtga tgatttacgg
    21121 cgagcgccgc tatgccatgc ggatctgggt ggaccgcgag cggttggcgg cctacgcact
    21181 gaccgttcag gacatcgaag atgctctgcg caatcagaac gtggagatcc catcgggacg
    21241 catcgagagc cgcgagcggg agttcagcgt cctttcgcga acgggattga ccactccgga
    21301 gcagtttcga cagatcgtgg tcaagcgggc cgatggctat caggtcaagc ttggcgatgt
    21361 cgcccgggtc gagctgggtg cggaggatga acgccgcgac agccggtaca atggccagcc
    21421 ggccgtcacc gtggggatca tcaagcaggc cgtggccaat ccgctcgatg tctcgaaggc
    21481 cgtccggggc atcctgccgg ctttgaacga gtccatgccg aatggcctca aggccgacat
    21541 cggcaacgac gacgctgtct tcatcaacga gtccatcaag gcggttttca ccaccatcat
    21601 ggaagccatc gtcctggtgg tgatcgtcat ctttttcttc ctgcgatcgg tgcgcgcctc
    21661 gctcatcccg atcgtcacga taccgatctc gctggtggcg acgttcacgc tcatcctcgc
    21721 attcgggttc agtatcaaca cactcacact gctcgcgatg gtgctggcga tcggcttggt
    21781 cgtagatgac gccatcgtgg tgctcgaaaa catatcaagg cacgtcgaaa gtggcctgcg
    21841 ccccatgcag gccgccatca aaggaacgcg cgaaatcggc ttcgccgtcc tcgcgatgac
    21901 gctgaccttg acggccgtct acgcaccgat cgcgtttgcg accggccgca cgggacggct
    21961 cttcatggag ttcgcactgg cgctcgccgg cgccgttctg gtctccggct tcgtcgcact
    22021 gacgctgacg ccgatgctgt gctcgaagct ccttcgccac aatgcatcac cggggcgcct
    22081 gttcactttc atcgaggatg tgctgaagcg gctcgagtcg ggctaccgcc gctcgctgga
    22141 gttcgccctc tccgtgcgct ggctggtggt ggctctcgcc attctcactg cgggcgccgc
    22201 cggcatgctg ttcgtcagcc tcaaatcaga gctgtcgccg actgaagaca ggggtgttct
    22261 actgatccgc gggtctgggc cggagggttc gacgctggcc tataacaagc gctacagcga
    22321 gcaggccgac gccatcttgg ccgaattgcc ggaggtgcgc tctcgcctga ccatcgacgg
    22381 cctctatcag caggttacca atttcatcgt catcggccgg ctgaccgatt ggtcggcgcg
    22441 ggatcgcaag cagcaggaac tggcggccga gatcggtccc aaggtccgaa ggatccctgg
    22501 ggtgctggcc ttcgctcaga atccggcttc tcttgggcag cgcggcggct cccggcccat
    22561 cgagtttgtt atccagacat ccggcaccta tgagcagctg gatgaatatg cggagaagat
    22621 gctggagcgt atccgctcct acccgggcct ggagggtgtc gataccgacc tcaagctcaa
    22681 ctcgcccgag ctgcgcatcg acgtggaccg ggccaagatc gccgacctcg gtttggacgt
    22741 ggccgtggtt gggcgcacgc tggagacgtt gctcgggggc cggcaggtta cccgtttcga
    22801 gcagaatggc gagcagtacg acgtcatcgt ccagctcgcc gccgaggacc gcagctcacc
    22861 ggaaaccctg tcgaccatct tcgtccgggg tgcgaatggt gagatggtgc agctgtcgaa
    22921 catcgtgacc gtcagcgaaa cggtcgcccc caaggagctc aagcgcttca atcagctgcg
    22981 ctcggtgacg atccaggcga atctggcgcc cggctactcg ctgggtgaag gacttgaatt
    23041 tttggagaac acggcgcgcg aagtcctccc ctcccatgtc cagacggact tgaacggcca
    23101 gagccgcgag ttccgctccg cggggcagag ccttgcgttc gtgtttttgc tggcgctcgg
    23161 cttcatctat ctcgtgctgt cagctcaatt cgaaagcttc cggtcgccgc tcatcatcat
    23221 ggtgaccgtg ccactctcca tgaccggcgc gctggcggcc cttcacctta ccggcgggac
    23281 gctgaacgtg tactcgcaga tcgggctcgt gacgctgatc gggctgatta ccaagcacgg
    23341 cattctgatc gtcgatttcg ccaaccagca gcaggaggcg ggcagggata tgcgcaccgc
    23401 cgtgctcaat gccgccgccc tgcgcctgcg gcccattctg atgacaaccg gcgccatggt
    23461 gctgggggcg gtgccgctgg cccttgccac cggcgcaggg gccgagagcc ggcagcagat
    23521 cggctgggtc attgccggag gcatgacact gggtacgatt ttgacgcttt tcgtggtgcc
    23581 tgtggtctac atgctcatcg gccgcaatta cagcctgata aagcacgagg aggagctggc
    23641 cgatcacccc caaccggccg aatagctgga aagggcagcc gcagccattt cggccggggc
    23701 gtcgacgtgt cggccgcccc caattcatgt tctgccggcg ggcagacctg cttgggctat
    23761 ttcactctcg tgtactttgc ggtcatgatg gtctgccact ttccatcggc gcccgaaacg
    23821 gacgaagtga agaccctgtg atccgggtct ttgatttcga tcacctcccg atatttcgcc
    23881 attttgcctt cttcggtgca actcgggccc tcggtatcca ggctcagaat tttgccggtc
    23941 gggtcgaggg atccttcgta aacccacagg taggtcatca tcgagccgag ccaggtgccg
    24001 acgaattttt ccttttgcgg atcgtatcca agggtcagca tcatcaccga tggttgcccg
    24061 tcgggcattt ggctgcgacc ctccgcctga atccagagtc cgccgatgga tcgaacggtt
    24121 tctgttccgg tatgattgca gtcgggatct ttcgacgggg cctcgaagcg ccagtcacca
    24181 acgagctgct gcagccactc gtgctctttc tgcggttttg ccgattccat tctttccctg
    24241 cctgctttgc tgccctagaa aactagccgc ccgaaatcgc cgtcagcacc cagacctggg
    24301 ctcccgattc caggtgcgtg tcgagagagg cgcgctcgcc attcacgaag acgttgatgt
    24361 ggcggcgaac ggcgggcgtc gagtcgcaga ggcggtcgcg cattcccggc cagcgatcat
    24421 cgagtgcatc catcacctcg ccgacggtcg aagcctcgac atcgacgcga cgcgctgcct
    24481 ccggaaacag ccgcaatagc gcgggcgcaa ggatcactgt gaccgtcggc tgcaagaccg
    24541 cggacgcatt cggcgtcatt ggcaaagctc caaagctcgt tcagctgtcg atgaccatcg
    24601 tctcgaccga ggtgatcgtc ggcagatgga gcgcgatgca attccagctt tcgccctcat
    24661 ccgcgctggc gaaaagcgcg cctgagctcg tgccgaaata aacgcctgcc ggctcagcag
    24721 gatcggtcgc catggcctga cgcagcacac ccaaaaaggc attcttttgc ggcaagcctt
    24781 cgcgcaaatc ctgccatgtt ttacccgcat cgcgcgtccg ccaaacggcc gttttcccat
    24841 cgggaacgaa acgcccggcg atgtcgccgt tgaggggaag caaatagagc gtatccgggt
    24901 cgcggggatg caccgcggcg ggaaagccga acgtcgatgg caagccctct tcaatgctca
    24961 cccagttcct gccgccgtcg tcgctgcggt acattccgca gtgattctgc tgatagagtc
    25021 ggtccggcat ccctggcgcc atcgccaggc agtgcacgca ctggccgaat tccggatacc
    25081 tctggtcttc cggcaggtag tcagcccgcg tgccgcggtt tcgcggctcc cagctttttc
    25141 caccatcagc cgtatgaaaa acgccggctg acgaaatggc gatccagagt tgatcgggat
    25201 cattcgggtg aagaacgatc gagtgcagga tgaggccccc tccccccggc tgccattcag
    25261 gccgagacgg gtgatcgcga aggccggata catggctcca cgtttgccct tcatcctcgc
    25321 tgtagaacag cccagcgggc tcgacgccgg cgtaaagccg tccgccggaa ccgagagcaa
    25381 ggctccatcc ggccttgatc ggttgctcgc cttcaggata ggcaagcccc tggctggagt
    25441 gcgtccagct cttgccgagg tcggtcgact tccacacagc cggtccgaac cactcgttgc
    25501 caccagtcgc gtaaatcgtg cctgttcgag gatcgccgct gacgtgattg atcggccagg
    25561 tttcgcagaa tgggccgctc agctcccagg aattgcggcg ggcgtcgctc tccaggagaa
    25621 atgcaccttt ttttgtgccg acgagtacaa ggactttctt cgccatgcga ttccttccgt
    25681 tgtgctcccc acccggctcg aactgctcat cacgacttat tgtcactggg atggcggagg
    25741 gcctggacag aaagggttga tatcaacact atagagccat gtcaaagcca aatgagcttc
    25801 ctttcgagat cacgcccgta gtgcgtgacg cctgcctctg ccttcacgtg cagcgggcgg
    25861 cacgggcggt tgctcgccgg tatgacgagg cgctgcggcc cctggcgctg acaaacggtc
    25921 agttttcgct gatgatgtcg ctcaaccggc ctgagccccc aaccatgggc caagtctcgg
    25981 ctgtcctggc gatggatcgc acgacagtga cggccaatct caagccgctg gagaggcgcg
    26041 ggctggtcga ggtacagata gacgaccatg atcgccgcag ccgccggctg aagctgacgc
    26101 aggctgggcg gaagcttttg gccaaagcca tgccgatctg gaaaaagacg caagcgaaaa
    26161 acaaaacgct tcttccggat tcggatctcg aaaaactgct cctctacctc cgggcgattt
    26221 cctgatcagc ggccctgcat ggcgccgccg attggcccag gacgcagccc tcccgtccgg
    26281 ctcgcgcgct ttgcatccac cggatggcca tgcggcggga gggctgacaa cgggtttgct
    26341 gtcctgcgcc ccgcgcttgc tctcgcccgg gcgctgtgct gcattcggca ggaaagttca
    26401 gcctcgacca aggaaggaaa tgccaccgtg aacctgtcat tcatgctgaa ggaacgtgcc
    26461 gcggctggcc gacccatccg catcggccag gtgggagccg gcaagttcgg caccatgttc
    26521 ctggcacagg cgcgaaccac gaccggcatg cacgtggccg ccgtggccga cctcaacatt
    26581 ccgcgcgcgc gctcgcagct caaaatggcg tcctggccgg aggaacagta cggagcgggg
    26641 tcgctcgacg aggcgctgaa aacgggcaag acgtttctga cggaagacgc cgagacgatg
    26701 atccggcatc cggccatcga cgtcgtcgtc gaagcgaccg gcgatcccag gacgggtatc
    26761 cgcctctgcc aactcgccat cgagaacggc aagcacatca tcatggtcaa cgtcgaggcc
    26821 gatgtcgtcg ccggtccgct gctcgccagg aaggcgcggc aggcgggcgt ggtctacagc
    26881 ctcgcgtggg gtgatcagcc cgctctcgtt tgcgagcagg tcgactgggc gcgagcgtgc
    26941 gggttcaagg tggtggccgc gggcaaaggc acgcggtatc tgccgagcta tcacaagctg
    27001 acgccggaga cggtctggaa cgagcttcag aaatatctga agatcgacga tcccaagagc
    27061 ataaacctga agatgttcaa cagcttcctc gatggcacca aatccggcat cgagatgagc
    27121 gcgatttgca atgcgacggg gctggagtcg cagactgacg gattgacctt tccgccggcg
    27181 actcgtttcg agcatgccga catctgcaag cccaagagcg agggcggagt tctcgagaaa
    27241 gccggcgtca cggaagtgac atcgatggtt tatcgcgacg gttccgacgt gccgcacaac
    27301 ctcgccatgg gcacctatgt cgttttcgaa gccgccgagg acaatgacta cagccgccgc
    27361 tgcttcaagg aatacaatct gctgccggac aagtcgggcc gctacgctgc tctctatcgg
    27421 ccgatccaca tgatcgggct ggagcttggc atttcgatcg cctcggcagt gctgaggaag
    27481 gagccgacgg gcgcaccaat ctgcttcaac tccgacgtgg ttgcaacggc aaagcggaat
    27541 cttactgcgg gcgaaattct tgatggcgag ggcggattct gcgtctgggg aaagcagacg
    27601 ccagcttcga tctcgctcaa ggaaggatat ctccccctcg gcctcgccca caatgtgaag
    27661 ctcaagcgcg atatccctga agggcagcgg ctcaggtggg acgatgtggc catcgatccc
    27721 gatgacgccg cagtcaaggc gcgccgtgaa atggaggccg ctttcggtcg gctgaatgaa
    27781 ggcccatgag gtaaatcaca gaaggtattc cgtttccggt t
//
LOCUS       DASVDU010000136        16771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_946213, whole genome shotgun sequence.
ACCESSION   DASVDU010000136 DASVDU010000000
VERSION     DASVDU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16771
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946213"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VF226_08615"
     CDS             <1..304
                     /locus_tag="VF226_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7074087.1"
                     /translation="RKVLEGEIWVPPEIDIEGQAEETSELVARLSTLTPQQVRVLMML
                     GEGLLNKQIAYRLGVSEATIKAHVSAILQKLGVDSRTQAVIAINKLDSGDWQRNAN"
     gene            complement(364..2820)
                     /gene="gyrB"
                     /locus_tag="VF226_08620"
     CDS             complement(364..2820)
                     /gene="gyrB"
                     /locus_tag="VF226_08620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX7074088.1"
                     /translation="MSAQPARKHNGAEHYGAENIKVLRGLDAVRKRPGMYIGDTDDGS
                     GLHHMVYEVVDNAIDEALAGFATEVQVILNVDGSCTVIDNGRGIPVGIHPEEGISAAE
                     VVMTQLHAGGKFGAGGDENPYKVSGGLHGVGVSVVNALSAWLELTIWRDGKEHFVRFV
                     NGVAEAPLRVVGDAEGRTGTKVTFLPSLETFRGVTEFSFETLEHRLRELAFLNSGVRV
                     VLIDDRHADRKQEVLQYEGGLGEFVRYLDRAKHPLVDTPIMIAAERDGVGVEAALWWN
                     DSYHENVICFTNNIPQRDGGTHLAGFRAALTRVINKYADETGIAKKEKVNLTGDDARE
                     GLTCVLSVKVPDPKFSSQTKDKLVSSEVKPVVESVIGDQLAAWFEEHPKEGKAIITKV
                     VEAAAAREAARKARELTRRKGALDMANLPGKLAECQDRNPANTELFIVEGDSAGGSAK
                     QARNREFQAVLPIRGKILNVERARMDKMLSSQEIGTLITALGTGIGRGEDGGFDLSKL
                     RYHKIIIMTDADVDGAHIRTLLLTFFYRQMPELIEKGHLYIAQPPLYKVTKGRSETYL
                     KDQASLEDYLIDSGLTDTVLELGTGETRAGPDLRDIVLQARVVQRILDGMHSRYARFV
                     VEQAAIAGALDAALLNDRPAAEDKAKAVAALLDSIAEETERGWTGRFSDNEGFVFTRE
                     VRGVKEVHVLDRAFIGSLDARRLNERHAHLAEIYEKPAVLKRKDAETTIYGPSSLLKA
                     VYDIGRKGLSLQRYKGLGEMNADQLAETTLDRDTRTLLQVKLGDLAEADEIFSKLMGD
                     VVEPRREFIQEHALLASVDA"
     gene            complement(2989..4158)
                     /gene="recF"
                     /locus_tag="VF226_08625"
     CDS             complement(2989..4158)
                     /gene="recF"
                     /locus_tag="VF226_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX7074089.1"
                     /translation="MSKPQRSIWIEHLTLTNFRNYASARLDLTPEPIVLFGANGAGKT
                     NLLEAVSLLAPGQGLRRAPFGQLARVGGSGDWAVAARVHAPRGTVDIGTGRLPSQETD
                     AGARTGRLVRIDGIPQSGSGALADWVEMVWLTPAMDGLFTGPASERRAFLDRLTLCFD
                     PGYRTRAGQYERAMRQRNRLLQDGATEDALFSGLELIMAETGVAAAAARAQAAAALEA
                     TISTRRQRGPDSPFPWAKVALEGWIEERLANQPAVDVEDAYLAALRSGRERDRAAGRA
                     LIGPHRTDMLVEHGPKGMPAAVCSTGEQKALLIGLVLAHAELLAERAGGAAPVLLLDE
                     IAAHLDVFRRAALFDELLRLGSQAWMTGTDRHAFAALENRARFLHIDEGRILEAA"
     gene            complement(4236..5351)
                     /gene="dnaN"
                     /locus_tag="VF226_08630"
     CDS             complement(4236..5351)
                     /gene="dnaN"
                     /locus_tag="VF226_08630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7074090.1"
                     /translation="MRLVIERSELLRALGHVTSVVERRTTIPILSNVLLRASGSQLEL
                     KATDLEREVIERAPAEISAPGAVTVPAHMLHDIVRKLPDGAEVEMVRDPERDRLALTS
                     GQARFALQTLPAEDFPDLAAGEMSHEFKIAAADLKKLLDKTRFAISTEETRYYLNGIY
                     FHIASNTEPTLRAVATDGHRLAQIELPRPAGADGMPGVIIPRKTVHELHRLLEDSEVE
                     VRVGVSTSKVRFEIGTVTVTSKLIDGTFPDYGRVIPQHNDKVLTVSNSQFMAAVDRVS
                     TIASERGRAVKLNLSDDRMVLSVNNPEGGSATEELGVSYSAAPLEIGFNARYLLDIAS
                     QLESDSAVFELADPGSPTLVRDGDDESALYVLMPMRV"
     gene            complement(5631..7193)
                     /gene="dnaA"
                     /locus_tag="VF226_08635"
     CDS             complement(5631..7193)
                     /gene="dnaA"
                     /locus_tag="VF226_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX7074091.1"
                     /translation="MNSIEPTRPAARVASSAGESKPRSEAHSLSRTGPAASSGTQEPR
                     GHKVRKMLRAQLGEDVYTSWFHSMEFEHFDGQTVHVTVPVKFLKNWIQSHYSEALLKC
                     CAVEFEGVERVEVALRQPSPVRTQASEPASDTDSRPVTGGHGSAAPALNNWQVPRPVM
                     PTMTTRTQVGGFEGSPLDPRYTFDSFAVGPSNRMAHAGATQVAETVLSDSRGFNPLFI
                     HSAVGLGKTHLLHAIAWSVRRRVPQAQVLYLTAERFRYQFVEALKSQDPLSFKEKFRS
                     IHMLLIDDLEFLHGQQTEQEFDHIINSLLDGGRQVVVASARAPNQIEKLNERMRSRLQ
                     RGLVTEIGAPDAELRLRILEKRVEEKRQLDPSFEIHRDILELLADRLHESGRELEGAV
                     TRLHATWQYMRTPITMDIAETVIRDLVHGIEPRRIKIEDILKVVSRHFGVSKGDILSQ
                     RRHRSVVWPRQIGMYLAKQLTSRSLPEIGRRFGNRDHTTVLHAIRKIEGELGGNPRLR
                     DELDELKKMLNS"
     gene            complement(7295..7738)
                     /locus_tag="VF226_08640"
     CDS             complement(7295..7738)
                     /locus_tag="VF226_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7074092.1"
                     /translation="MGSGQAIHIVDVDVRDVWKRLKEEPAAVLIDVRTRAEWAYVGLP
                     DLSSIGKQPILIEWQTFPDNRVDPDFVDRLSSMLETAGVDKEAELFFICRSGGRSMRA
                     AQAMAAAGYSACRNVADGFEGPLDADRHRGKAAGWKAAGLPWAQG"
     gene            complement(8379..8645)
                     /gene="rpsT"
                     /locus_tag="VF226_08645"
     CDS             complement(8379..8645)
                     /gene="rpsT"
                     /locus_tag="VF226_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7074093.1"
                     /translation="MANTSSAKKAVRKTARRTEINKARRTRMRTEVRKVEEAIASGQK
                     EAALEALRAAEPILARTAQKGVIHKNAASRKISRLAARVKAMPA"
     gene            8953..9966
                     /locus_tag="VF226_08650"
     CDS             8953..9966
                     /locus_tag="VF226_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX7074094.1"
                     /translation="MRILVTGAAGFIGYHTAEALLARGDSVIGLDNLNPYYDVRLKEA
                     RLDRLRRHRQFQFAKVDLADRKAMEEVFAAHRPQRVAHLGAQAGVRYSLENPHVYVDS
                     NVVGTLHVLEGCRAHGVEHLVLASTSSVYGANTKMPFSVEDKVDHPLSLYAATKKSCE
                     LMAHTYAHLYGLPVTALRFFTVYGPWGRPDMALFKFTRAILAGEPIEIYNNGQHARDF
                     TYIDDIVEGVVRTLDRPAKPDPAWSGDRPNPATSLAPYQLYNIGNNRPVELMDLVACL
                     ERALGRTAVKTFLPLQPGDVPRTAADIERLARDVGFRPNTPIEVGVERFVAWYREYYG
                     LTS"
     gene            10000..10599
                     /locus_tag="VF226_08655"
     CDS             10000..10599
                     /locus_tag="VF226_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074095.1"
                     /translation="MSTLVPLIVAGLSLVFLVFAADARAVDVRMAYVHAAIAAATALL
                     VAGIAVRQCRTLLAKAASASEIASSNAWYMGLIWGWGMLALIVIYGTGVLVWKEWWHF
                     VLAFGLAAALSLYFARVLKNDALAGKEDETMLRIARYLAIVQLAGMVITMLGLIIDGK
                     MVRFLTPRFTDWAANNIFFFGALGIAVISAYALQHNKHA"
     gene            10658..11272
                     /locus_tag="VF226_08660"
     CDS             10658..11272
                     /locus_tag="VF226_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074096.1"
                     /translation="MSLQRVLPWLVLALFASVVVMISGAGRDQSWTTGAAAAAFAVLA
                     VSIGFGLNQPLWRLEPFRIAPEAAPVAAQRNAKLMALAWAWGAAAMAGVYTLGGLRWQ
                     HAWQYGSGMALIAIFTWGFGLAIARAERPLTRQMLLLRGLQLTIVQGVAATGGVIYLL
                     AAGKLMSFRSDWAANQIFLAGGVTIAALSAMAVITQRRLTCRQP"
     gene            complement(11282..12055)
                     /locus_tag="VF226_08665"
     CDS             complement(11282..12055)
                     /locus_tag="VF226_08665"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7074097.1"
                     /translation="MAYQNIIVETRGKVGLITLNRPQALNALNSALMAELNQALDAWE
                     ADDSIGCMVITGSEKAFAAGADIKEMQSKSYMDVYLGDFIGTWDRVARCRKPVIAAVA
                     GFALGGGCELAMMCDFIIAADTAKFGQPEIKLGVMPGAGGTQRLARFIGKAKAMDLCL
                     TGRMMDATEAERCGLVSRIVPAADLIEEAVKAASTIADMSLPAAMMTKESVNRAFETT
                     LAEGVRFERRVFHAMFATEDQKEGMAAFVEKRKPVFKNR"
     gene            complement(12116..13003)
                     /gene="mutM"
                     /locus_tag="VF226_08670"
     CDS             complement(12116..13003)
                     /gene="mutM"
                     /locus_tag="VF226_08670"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX7074098.1"
                     /translation="MPELPEVETVRRGLEPVLAGHRIVRVDQRRPDLRFPFPENFAER
                     LQGQTICTLGRRAKYLLARLSGGEVLVMHLGMSGRFTITAPCSGSAVPLGDFVYDIGD
                     DPAHDHVVFTLENGTTITYHDPRRFGFMLLVPEAMLEQHPLFAKLGVEPLGNELSPAY
                     LAQKARGRKADLKAFLMDQRIIAGLGNIYVCEALYRARLSPLRPAASLATTTGKPTPR
                     AERLVEAIRSVLRNAIDKGGSTLRDYRQANGAEGEFQHLFYVYGREGEPCLTPGCRGI
                     VQRIVQAGRSTFYCAKCQK"
     gene            13224..13994
                     /gene="ubiE"
                     /locus_tag="VF226_08675"
     CDS             13224..13994
                     /gene="ubiE"
                     /locus_tag="VF226_08675"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7074099.1"
                     /translation="MARNEQHDTAASFGFREVRPEERQGLVNQVFATVADRYDLMNDL
                     MSGGLHRLWKDELVTMLNPPASEQPFRLVDVAGGTADVALRMLRKGGPGCSAVICDIS
                     PEMLRVGRSKVEAEGRSEQVSLVQGNAEALPFPDKSFDAYTIAFGIRNVTHIDTALGE
                     AYRVLKPGGRFVCLEFSECQVPMLDKLYDLHSFAVIPRLGQLVGTGAEPYRYLVESIR
                     KFPAQEPFAAMIREAGFERVTWRNLTGGIAAIHSGWRI"
     gene            14003..15598
                     /gene="ubiB"
                     /locus_tag="VF226_08680"
     CDS             14003..15598
                     /gene="ubiB"
                     /locus_tag="VF226_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEX7074100.1"
                     /translation="MASAPMNLIRLARAGFVLAQHGVQFVPPGMKAPLALKLAHALTW
                     PVRMISAPFQAGKPRDRRITDALASLGPSYIKLGQFLATRADLIGPELASDLCHLQDK
                     LPPFSMEEARRAVEEALGGKLETHFAEFGPPVAAASIAQVHKAVVIENGERRKVAVKI
                     LRPGIERRFKRDLDSYYFAARAIERFHPPSRRLRPVAVVDTLARTTQLEMDLRLEAAA
                     ISEMAENSAADAAEFRVPQVDWRRTAKRVLTVEWIDGIPISDRVALQAAGHDLGQLGQ
                     IVLRTFLRHAMRDGFFHADMHQGNLFVDEMGRIVAVDFGIMGRLGMKERRFLAEILHG
                     IINRDYKRAADVHFWAGYVPPHHPVAVFAQALRAIGEPIHGRTASEISMADLLGQLFA
                     YTEVFDMQTRPELILLQKSMVIVEGVARGLDPNLNLWTAAEPVAREWVEANYGVSGRL
                     REAGEGAEVVGKILSDLPNLLERVEQGAAAFAEMGRNGIRLDVETVERIGAAEARHTR
                     WGRVALWVGALSLVAMAVVMIAG"
     gene            16065..16451
                     /locus_tag="VF226_08685"
     CDS             16065..16451
                     /locus_tag="VF226_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX7074101.1"
                     /translation="MNVFARLSASPPDRNQISFTFDGGITFDGVIPGRPDDVLGVGFS
                     YSRISPSLSALDRDTLRAGTYTLVQDYEALIEFSYRAQIVPGWTIQPDVQYMWHPGGH
                     VPINSAPGAPAIPSATIVGVRTAINY"
BASE COUNT         2853 a         5369 c         5469 g         3080 t
ORIGIN      
        1 gcgcaaggtg ctggaagggg aaatctgggt gccgccggag atcgacatcg aggggcaggc
       61 ggaagaaacc agtgagctcg tcgcgcgcct gtcgacgctg acgccgcagc aggtccgcgt
      121 gctcatgatg ctgggggagg gattgcttaa caagcagatc gcctaccgcc tcggcgtgtc
      181 ggaggcgacc atcaaggctc acgtatccgc gatcctgcag aagctcggcg tggacagccg
      241 tacgcaggcc gtgatcgcca tcaacaagct cgacagcggc gactggcaac ggaacgcgaa
      301 ctgaccgccg gccgggccga aggtcagcgg aaggcgtagc agaccaggcc gaaggccgtc
      361 cgatcaggcg tcgacgctcg ccaggagcgc gtgctcctgg atgaactcgc gccgtggctc
      421 cacgacgtca cccatcagct tcgaaaagat ctcgtccgcc tcggcgagat caccgagctt
      481 cacctgcagc agggtgcgtg tatcgcggtc gagggtcgtt tccgcgagct gatccgcgtt
      541 catctcgccg agtcccttgt agcgctgcag ggacagccct ttgcggccaa tgtcgtagac
      601 ggccttgagc aggctcgacg ggccgtagat cgtggtctcg gcgtccttgc gcttgagcac
      661 tgcgggcttc tcgtaaattt ctgcgaggtg agcgtggcgc tcattgaggc ggcgtgcgtc
      721 gagcgagccg atgaaagcgc ggtcgagcac gtgaacctct ttcaccccgc gcacctcgcg
      781 agtgaaaacg aagccctcgt tgtccgagaa tcgtccggtc cagccgcgct cggtctcctc
      841 ggcaatggaa tccagtagcg ccgcaacggc tttcgccttg tcctccgcag ccggcctgtc
      901 gttgaggagc gccgcatcga gggccccggc gatggcagcc tgttccacga cgaaccgggc
      961 atagcgcgag tgcatgccgt cgaggatgcg ctggacgact cgtgcctgca ggacgatgtc
     1021 gcgcaggtcg gggccggcgc gtgtttcgcc ggtgccaagc tcgaggacag tatccgtgag
     1081 cccgctgtcg atcagataat cctcgaggct ggcctggtcc tttaggtagg tctcggaccg
     1141 gcccttggtc accttataga gcggcggctg ggcgatgtag aggtggccct tctcgatcag
     1201 ctccggcatc tgacgataga agaacgtcaa cagcagcgtg cggatgtgcg cgccgtcgac
     1261 gtcggcgtcc gtcatgatga tgatcttgtg gtagcgcagc ttcgacaggt cgaagccgcc
     1321 gtcctcgcca cggccgatgc ccgtgccgag cgccgtgatc agcgtcccga tctcctggct
     1381 ggacagcatt ttgtccatgc gcgcacgctc gacgttgagg atcttgccgc gaatgggcag
     1441 gacagcctgg aactcgcgat tgcgcgcctg cttggccgag ccgccggccg aatcgccctc
     1501 gacgatgaac agctccgtat tggccgggtt gcggtcctgg cactcggcga gcttgccggg
     1561 cagattggcc atgtcgagcg cgcctttgcg gcgggtcagc tcacgtgcct tgcgcgcagc
     1621 ctctcgcgcg gcggccgcct cgaccacctt cgtgatgatg gccttgcctt ccttcgggtg
     1681 ctcctcgaac caagccgcaa gctgatcgcc gatgacgctc tcgaccaccg gcttcacctc
     1741 ggacgagacg agcttgtcct tggtctggct ggagaacttg gggtcgggca ccttgacgga
     1801 cagcacgcag gtgaggccct cgcgcgcatc gtcgcccgtg agattgacct tctccttctt
     1861 cgcgatgccg gtctcgtcgg cgtacttgtt gatcacgcgc gtgagcgcgg cgcggaagcc
     1921 ggccagatgg gtgccgccgt cgcgctgcgg gatgttgttg gtgaagcaga tcacgttctc
     1981 gtggtagctg tcgttccacc acagcgcggc ctcgacaccg acgccgtcgc gctcggctgc
     2041 gatcatgatc ggcgtgtcga ccagcggatg cttggcccga tcgagatagc gcacgaactc
     2101 gccgaggccg ccttcgtatt gcaggacctc ctgcttgcgg tccgcatggc ggtcgtcgat
     2161 cagcacgacc cgcaccccgg agttcaggaa cgccagctcg cgcagccggt gctccagcgt
     2221 ctcgaagctg aactcggtca caccacggaa cgtctcgagg ctcggcagga aggtgacctt
     2281 ggtgcccgtg cggccctccg cgtcccccac cacgcggagc ggcgcttcgg ctaccccgtt
     2341 gacgaaacga acgaagtgct ccttgccgtc acgccagatg gtgagctcca gccaagccga
     2401 aagcgcgttg acgaccgaca cgccaacgcc gtgcaggccg cctgagacct tgtagggatt
     2461 ttcgtcgccg cccgcgccga acttgccgcc ggcgtgaagc tgcgtcatca ccacctcggc
     2521 ggctgaaatg ccttcctcgg ggtgaattcc aacgggaatg ccgcgtccgt tgtcgatgac
     2581 cgtgcacgaa ccatcgacgt tcagaatgac ctgaacttcc gttgcgaatc cggccagcgc
     2641 ctcgtcgatg gcattgtcga ccacctcgta gaccatgtga tgcaggccag agccgtcatc
     2701 ggtgtcgccg atgtacatgc cggggcgctt gcgcacggcg tcaaggccgc gcagcacctt
     2761 gatgttttcc gcaccatagt gttcggcgcc gttatgtttc ctggccggct gagcactcat
     2821 cgaagactgt tcctgtgggg aagtaatgtc gagacgtttc ttatagcaca gatcgagcgg
     2881 aatgaggccg gaatcgtgct ccttatagat ggggatcgac agaaaaattg caagattttt
     2941 cagttgcttg gcgaagctgc gtcagcggaa cacacgggct ttggcgcttt acgcagcctc
     3001 caggatccga ccctcgtcga tatgcaggaa acgggcccga ttctccagcg ccgcaaaggc
     3061 atgccggtcc gtccccgtca tccaggcctg agagcctagc cgcaacagct cgtcgaacag
     3121 cgccgcccgc cggaaaacgt cgagatgggc tgcaatttcg tcgagcagaa ggaccggcgc
     3181 cgcacctccg gcccgctcgg ccagcagctc ggcatgggcc agaacgagcc caatcagaag
     3241 agccttctgc tcgcctgtcg agcagaccgc agccggcatg cccttcggac cgtgctcgac
     3301 gagcatatcc gtgcgatgcg ggccgatgag cgcccgtccg gcagcccgat cccgctcgcg
     3361 cccgctgcgc agcgcggcca gataggcatc ctccacgtcg accgcgggct ggtttgccaa
     3421 ccgctcctcg atccagcctt caagggccac cttcgcccag gggaacggcg aatcggggcc
     3481 gcgctgccgt ctcgtggaga tggtcgcttc cagggcagcg gcagcctgtg cgcgcgcagc
     3541 agctgctgcg acacccgttt cggccatgat cagctcgagc cccgaaaaga gggcatcttc
     3601 cgtcgcgccg tcctgcagca ggcgattgcg ctgtcgcatc gcccgctcgt actggccggc
     3661 gcgtgtccgg tagccggggt cgaagcagag tgtcaggcgg tcgaggaagg cgcgtcgctc
     3721 gcttgctgga ccagtgaaca ggccgtccat ggcaggggtg agccacacca tctcgaccca
     3781 gtcggcaagc gcaccggagc ccgactgcgg aatgccgtcg atgcgaacca ggcggccggt
     3841 tcgtgcaccg gcgtcggttt cctggctggg aaggcgcccg gtgccgatgt cgactgtccc
     3901 gcgcggtgcg tgcacgcggg cggcaactgc ccaatcccca gagccaccaa cgcgcgcgag
     3961 ctgcccgaag ggagcgcggc gcagtccctg gccgggtgcg agcaaagaca ccgcctccag
     4021 gaggtttgtt ttgccggccc cgttggcgcc gaacagcacg atcggttccg gggtgaggtc
     4081 cagccgggcg cttgcgtagt tgcggaagtt cgtcagggtg aggtgctcga tccagatcga
     4141 ccgttgcggc tttgacatcg acctcctggc ggcaccgtcc ggtttcggcc cggcattcaa
     4201 cctcgaaact cctcaccagc ggcaggcccg ccgcctcaga cgcgcatcgg catcagcacg
     4261 tagagcgcgc tctcgtcgtc gccgtcgcgc accagcgtcg gcgagcccgg atcggccagc
     4321 tcgaacacgg ccgaatcgct ctcgagctgg cttgcgatgt ccagcaaata gcgggcattg
     4381 aagccaatct cgagcggggc tgcggagtaa ctgacgccca gctcctcggt tgcgctaccg
     4441 ccttcggggt tgttgaccga aagcaccatc ctgtcgtcac tgaggttcag cttcacggcc
     4501 cggccacgct cgctggcgat ggtggaaacg cgatcgacgg ctgccatgaa ctggctgttc
     4561 gagaccgtca gcaccttgtc gttgtgctgc gggatgacac gcccgtaatc ggggaatgtt
     4621 ccgtcgatca gcttggatgt caccgtgacg gtgccgatct cgaaccgcac cttgctggtc
     4681 gagacaccca cccgcacctc gacctccgag tcctcgagca gacgatgcaa ctcgtgcacg
     4741 gtcttgcgcg ggatgatgac gcccggcatg ccgtcggcgc cggccggccg cggcagctcg
     4801 atctgggcga ggcgatggcc gtccgtcgcc acggcgcgta acgtcggctc ggtgttgctc
     4861 gcgatgtgga aataaattcc attgaggtag tagcgcgtct cctccgtcga gatggcgaaa
     4921 cgcgtcttgt cgagcagctt cttgagatcc gcagccgcga tcttgaactc gtggctcatc
     4981 tcgccggcag cgaggtccgg aaaatcctcc gcgggcaggg tctgcaacgc gaatcgcgct
     5041 tgaccggaag tcagcgccag acggtcgcgc tccggatcgc gcaccatctc gacctcggcc
     5101 ccgtcgggca acttgcgcac gatgtcatgc agcatatgcg cgggcaccgt cacggcgccc
     5161 ggggccgaga tctctgcggg cgcccgttcg atcacctcac gctccagatc cgtcgccttg
     5221 agctcgagct ggctgccaga ggccctgagc aggacgttcg acaggatcgg gattgttgtg
     5281 cgccgctcga cgacgctggt gacgtggccc agtgcccgca ggagctcgct tctttcgatc
     5341 actagccgca tggcaacatg cctttcggag tgtgcccaaa tggatggccc tctggtggga
     5401 caagctcgtc aaatcgcaac ccggaccgct gcgtcaacca atagctaatc tgggaggatt
     5461 cggcgcgtag cagcaacttg accagagtca gccgccttgg caaggcccgg cagaaggccg
     5521 cgcccatttt gctaccaggc gattgagggt tgttggacgg aggagcggct cggggggacc
     5581 gctcctccgt cgagcaacgc ttcagggctc gatggccgag cccgtcggcg tcaggagttc
     5641 agcatcttct tgagctcgtc gagctcatcg cgcagacgcg gattgccgcc aagctcgcct
     5701 tcgatcttgc gaatcgcgtg caacaccgtc gtgtggtcgc gattgccgaa gcggcggccg
     5761 atctcaggca gtgaccgaga ggtcagctgt ttggcaagat acatgccgat ctggcgcggc
     5821 cacaccacgg aacggtgccg ccgctgggag aggatgtccc ccttcgagac gccgaagtga
     5881 cgcgagacca ccttcaggat gtcctcgatc ttgattcggc gcggctcgat cccgtgcacc
     5941 aggtcgcgga tcaccgtctc ggcgatgtcc atggtgatcg gcgtgcgcat gtactgccag
     6001 gtggcgtgca gccgcgtgac ggcgccttcc agctcgcggc cgctttcatg cagccggtcg
     6061 gccagcagct cgagtatgtc gcggtgaatc tcgaaagacg ggtcgagctg gcgcttctcc
     6121 tcgacgcgct tctcgagaat gcgaagccgg agctcggcgt ccggcgctcc gatttcggtc
     6181 accagaccgc gctgcaggcg cgagcgcatg cgctcgttga gtttctcgat ctggttgggt
     6241 gcccgggccg aagccactac cacctgccgc ccgccgtcga gcaacgagtt tatgatgtga
     6301 tcgaactcct gctccgtctg ctgaccatgc agaaactcga gatcatcgat cagcagcatg
     6361 tggatcgagc ggaacttctc cttgaaggag agtggatcct ggctcttcag ggcttcgacg
     6421 aactggtaac ggaaacgctc tgccgtgagg tagagcacct gggcttgcgg cacgcgcctg
     6481 cgcaccgacc aggcgatcgc gtgcagcagg tgggttttgc cgagcccgac cgcggagtga
     6541 atgaacagcg ggttgaagcc gcggctgtcg ctcaggaccg tttctgccac ctgggtggct
     6601 ccggcgtggg ccatgcggtt cgaggggccg acggcgaagc tgtcgaacgt gtagcgcggg
     6661 tcgagcgggg atccttcgaa gccgccgacc tgtgtccgtg tcgtcatcgt tggcatgacc
     6721 gggcgcggca cctgccaatt gttcagggca ggcgctgccg aaccgtggcc gccagtgacc
     6781 gggcgactgt ccgtatccga agccggctcg gatgcttgcg tgcgtacagg gctcggctga
     6841 cggagcgcca cttcgacccg ctcgacgccc tcgaactcga ctgcgcagca tttcagaagc
     6901 gcttcggaat agtgcgactg gatccagttc ttgaggaact tgacgggaac ggtcacgtga
     6961 acggtttggc cgtcgaaatg ctcgaattcc atcgagtgaa accagctcgt gtagacgtcc
     7021 tcgccgagct gggcacggag catcttgcgc accttgtggc cgcgcggctc ttgtgtgccg
     7081 gaggatgccg caggtccggt acgcgaaagg gagtgggcct cggacctcgg tttcgactcg
     7141 ccagcgctgc tcgcaacgcg tgcggcaggt ctcgtcggtt cgatactgtt catgagtaca
     7201 cccgctgctt tgtgtttgac cctctgttca atggcgtcgt gcacgcgacc gccgccacgg
     7261 tcgacacgcc aacgcttctt tgatttcagc ggcgctatcc ctgcgcccaa gggaggccgg
     7321 ccgctttcca gccagcggcc ttgcctcggt ggcggtcggc atcgagtggc ccctcgaaac
     7381 cgtccgcgac attgcggcag gctgagtatc ctgctgccgc catggcttgc gcagccctca
     7441 tactgcgccc gccggacctg cagatgaaaa agagctcggc ctctttatcg acgccggccg
     7501 tctccagcat gctcgagagg cggtccacga aatcgggatc gacccgatta tcggggaagg
     7561 tctgccactc gatcaggatc ggttgcttgc ctatggagga caggtccggc aacccgacgt
     7621 acgcccattc cgccctggtg cgcacatcga tgaggaccgc cgccggttcc tccttcagcc
     7681 gcttccacac atctcggacg tcaacatcca caatgtggat ggcctgccca gaacccactt
     7741 taaacctcca ctcatgcacg caacgctcgc cgttccgccc gttcagtacg ccaactgaag
     7801 caacgggacg gtgacgcaac gcacagccaa gctttcgccc ggaattacgc aggcatacag
     7861 aagaaaaaga acccgtctat tcaggcgcgc cggagtgccg aaactgctcc atcatggcgc
     7921 agtgcttacg caagacttga aacccatttt ggccgtcgtc cgccgaccga atggatctcg
     7981 tattaaccac acggaagggc gcgagacaag ctcactcagt cctcgtgctg tccgatacgg
     8041 ggcgctcggc gtctatcccc agcgctttgg aaaaacgggt gtgaagcagc aagactcgtg
     8101 gagaactcta ttgagtccac ggggagattc gcgtttgcca cacgccggcc caaaacaaat
     8161 cggcgccccc gagggagcgc cgattgaaca tcgttttctt ttgaaacccg gtgtaccgga
     8221 atttgacaac tcacccgcgc atgaggcgac cagctgccga gaatcctcga tatatgaagc
     8281 gcttttcaac tctcgttcga gcgactcacg gctttcgatc tccggcttgc cgttgcagat
     8341 ctgtcacagt cgccaaggca agaccaggaa gcgccatttc aggcgggcat agccttcacg
     8401 cgggctgcaa ggcgtgagat ttttcggctc gcggcattct tgtgaatgac gcccttctgc
     8461 gccgtacggg cgagaatcgg ttcggccgcc cgaagcgcct cgagggccgc ttccttctga
     8521 ccggaggcga tggcttcctc gaccttgcgg acctctgtcc tcattctcgt acggcgcgcc
     8581 ttgttgatct cggtgcggcg ggccgtcttg cgtacggcct tcttggccga gctcgtgttc
     8641 gccatagggt tcgttcctct tcggtacttg agacgccagg gccgtcacat ggatcacatg
     8701 ccggatctgg accggccttg gcagcagaat atgccaaacc gcccgggacc gtgcggtgcc
     8761 gggcggtaaa gtcgtagttg cctataacgc tgcttcgggc gagggtcaac ggcccacagg
     8821 agaaaggcgg ctgaagcggc ttgcgaactc gtcaacgcca gtgccaccac ctatagtggc
     8881 cacgacggca gccagccgcg caaggctttc cttgcccctg ccggctccaa ttggcatgtc
     8941 atgggaagca gaatgcgcat attggtaacc ggcgccgcag ggttcatcgg ctatcatacg
     9001 gccgaggcgc tcctcgcgcg cggcgatagc gtcattggac tagacaacct caacccgtac
     9061 tacgacgtgc ggctgaaaga ggcccggctc gaccggctgc ggcggcaccg gcagttccag
     9121 ttcgccaagg tcgatctcgc cgacaggaag gcgatggagg aggtctttgc cgcccatagg
     9181 ccgcaacggg tggcgcacct cggtgcccag gcgggcgtcc gctactcgct cgagaacccg
     9241 cacgtctatg tcgactcgaa tgtcgtcggc acgttgcacg tgctcgaggg ctgccgcgcg
     9301 cacggcgttg agcacctcgt gctggcgtcc acgagctcgg tctacggcgc caacacgaag
     9361 atgccgtttt ccgtcgagga caaagtcgac caccccttga gcctctatgc ggcgacgaag
     9421 aagtcctgcg agctgatggc gcacacgtac gcccatctct acggcttgcc ggtcacggcg
     9481 ttgcgcttct tcaccgttta tggtccttgg ggccggccgg acatggcgct cttcaagttc
     9541 acgcgggcga tcctcgccgg cgagccgatc gaaatctaca acaacgggca acatgcgcgg
     9601 gatttcacat atatcgacga catcgtcgaa ggtgtcgtcc gcaccttgga ccggccggcc
     9661 aagcccgatc ccgcctggag cggtgacagg cccaaccctg caacctcctt ggcgccttat
     9721 cagctctaca acatcggcaa caacagaccg gtcgagctga tggacctcgt cgcctgcctc
     9781 gaacgcgcgc tcggccggac tgcggtgaag actttcctgc cgcttcagcc gggggacgtg
     9841 ccgcggacgg ccgcagacat cgaacggctc gcaagagatg tcgggttccg gccgaacacg
     9901 ccgatcgagg tcggcgtcga gcggttcgtc gcgtggtatc gcgagtatta cggcctgacg
     9961 agctgacggc gggcaagcca accaccaacg ggggaatacg tgtctacact cgtcccgctc
    10021 atcgtcgctg gactaagcct agtcttcctg gtcttcgcag ccgatgcccg cgcagtcgac
    10081 gtgcgcatgg cctatgtcca tgccgcgatc gctgcggcga cggctttgct ggtggccggc
    10141 atcgccgttc gccagtgccg aacgctcctc gccaaggctg catccgcctc cgagatcgcg
    10201 agcagcaatg cgtggtacat gggcctcatc tggggctggg gaatgctggc gctgatcgtc
    10261 atctacggga ccggcgtgct ggtctggaag gagtggtggc atttcgtgct ggccttcggt
    10321 cttgcggccg cgttgtcgct ctacttcgcc cgtgtactga agaacgacgc tctcgccggc
    10381 aaggaggacg agacgatgct gcggattgcg cggtacctgg ccatcgtgca gctcgcgggc
    10441 atggttatca cgatgctggg cctcatcatc gatgggaaga tggtgcggtt tctgacgcct
    10501 cgtttcacag actgggccgc aaacaacatt ttcttcttcg gcgctctcgg gatcgccgtg
    10561 atcagcgcct atgcgctgca acacaacaag catgcctgat cctgcgcgag gcgctcgcat
    10621 ggaacgcggg cggcgcgcca accggacgga acgaaagatg tcgctgcaga gggtgctacc
    10681 ctggctggtc ctggcgctgt tcgccagcgt ggtggtgatg atttcgggcg ccggccgcga
    10741 ccaatcttgg acgacgggcg ctgcggcagc tgcgttcgcc gtgctcgccg tgagcatcgg
    10801 ctttggtctc aaccagccgc tctggcggct cgagccgttc cgcattgcgc ccgaggctgc
    10861 gccggtcgcc gcacaacgca acgctaagct gatggcgctc gcctgggcct ggggcgcggc
    10921 ggcgatggcc ggcgtctaca cgctcggcgg cttgaggtgg cagcacgctt ggcaatacgg
    10981 gtccggcatg gccctcattg cgatcttcac ctgggggttc gggcttgcca tcgcccgcgc
    11041 ggagcggcct ttgacacgcc agatgctgct gctgcgcggc ctgcaactca cgatcgtgca
    11101 gggcgtcgct gcaactggcg gagtcattta tctgcttgcc gcaggcaagc tgatgagctt
    11161 ccggtccgat tgggctgcca atcagatctt cctcgccggc ggcgtgacca ttgcggcgct
    11221 gtccgcaatg gccgtcataa cccagcgcag gctcacgtgc cgccaaccgt agcgcgacgc
    11281 ctcaacggtt tttgaaaacg ggttttcgct tctcgacgaa cgcggccatg ccctccttct
    11341 gatcctcggt cgcgaacatg gcgtggaaga cgcgccgctc gaaccggacg ccttccgcca
    11401 acgtcgtctc gaaggcgcga ttgacgcttt ccttcgtcat catggcggcc ggcagcgaca
    11461 tgtctgcgat cgtcgaagcc gctttcaccg cctcctcgat gagatcagcc gccgggacga
    11521 tgcggctgac gagtccgcag cgctcggctt cggtcgcgtc catcatgcgg ccggtgaggc
    11581 agaggtccat ggccttcgcc ttgccgatga agcgggcgag ccgctgggtt ccgccggcgc
    11641 cgggcatgac gcccagcttg atctcgggct ggccgaactt ggccgtatcg gcggcgatga
    11701 tgaagtcgca catcatggca agttcgcagc cgccgccgag ggcaaagccg gcaacggcgg
    11761 cgatcaccgg cttgcggcaa cgcgcgaccc ggtcccaggt gccgatgaag tccccaaggt
    11821 agacgtccat gtaggacttc gactgcatct ccttgatgtc ggcgccggcg gcaaaggctt
    11881 tttcggatcc ggtaatgacc atgcaaccga tgctgtcgtc cgcctcccac gcatcgagcg
    11941 cctggttgag ctcggccatc agcgcgctgt tgagcgcatt gagcgcctgc ggccggttga
    12001 gggtgatgag accaactttg ccacgggtct cgacgatgat gttctggtaa gccatcgatc
    12061 ggttctcact tgatctgtcg aatgatctct cgaagggcct gccgcgcggg attcgctact
    12121 tctggcactt cgcgcagtag aaggtggagc ggcccgcctg cacgatgcgt tgcacaatgc
    12181 cccggcatcc cggcgtgagg caaggctctc cttcgcggcc gtagacgtag aacaggtgct
    12241 ggaattcgcc ttcggcaccg ttcgcctgcc ggtaatcgcg cagcgtcgag ccccctttgt
    12301 cgatggcgtt gcgcagaacg gaccggatcg cttctacgag ccgctcggcc cgtggtgttg
    12361 gcttgccggt ggtcgtggcc aggcttgccg ccgggcggag ggggctgagc cgcgctcgat
    12421 aaagagcctc acagacgtag atgttgccga gcccggcgat gatcctctgg tccatgagga
    12481 acgctttgag gtcggctttc cggccgcggg ccttttgggc cagataggcc gggctgagct
    12541 cgttgccgag aggctcgacg ccaagcttgg cgaacagcgg atgctgctcc agcatggcct
    12601 cgggcaccag cagcatgaac ccgaaccggc gcgggtcgtg ataggtgatg gtcgtgccgt
    12661 tctcgagtgt gaagacgacg tgatcatgtg ccggatcatc gccgatgtcg tagacgaaat
    12721 cgccaagcgg gacggcgctg ccggagcacg gagcggtgat ggtgaaccgg ccgctcatgc
    12781 caaggtgcat gacaaggacc tcgccgcccg acaaccgcgc cagcaggtac ttcgccctgc
    12841 ggcccaacgt gcagatggtt tggccttgca ggcgctccgc gaagttctcc gggaacggga
    12901 agcgcaggtc cggacgccgc tgatccacgc ggacgatgcg gtggccggcg agcacgggct
    12961 cgagtccgcg ccggactgtt tccacctcgg gcagttctgg caacctgctg ctccccgttt
    13021 gctgtcggcg tgagcagcat tagccccgat ggtcgcgccg cgcgagaccc gctgccgccg
    13081 cggcagggga ttgcccctaa gcgctgccgt taaccgtttg atatgcttgg cagaccacat
    13141 tgggcctgcg agatcccgcg actctggctt gcggccgttt ccgcacatgg cgcttgcctg
    13201 atcgctcaga tatggtgccc gccatggcac gcaacgaaca gcacgatacg gcggcgagct
    13261 tcggctttcg cgaggtccgg cccgaggagc ggcaggggct cgtcaaccag gtcttcgcga
    13321 ccgttgccga ccgctacgac ctgatgaacg acctcatgtc cggcggcttg caccggctct
    13381 ggaaggacga gctcgtgacc atgctcaacc cgcctgcgtc ggagcagccc ttccggctgg
    13441 tcgacgtcgc gggcggcacc gccgacgtgg cgcttagaat gctgcggaaa ggcggcccgg
    13501 gctgctcggc cgtgatctgc gacatcagcc cggagatgct gcgggtcggc cgcagcaagg
    13561 tggaggcgga ggggcgctca gagcaggttt cgctggtcca gggaaacgcg gaggcgctgc
    13621 cgtttccgga caagagcttc gatgcgtata cgatcgcatt cggcatccgc aacgtcaccc
    13681 acatcgacac ggcactcggc gaagcctatc gcgtgctgaa gcccggcggt cgcttcgtgt
    13741 gcctcgagtt ctcggaatgc caggtgccga tgttggacaa gctttacgac ctgcattcct
    13801 ttgcggtcat cccccggctc gggcagctcg ttggcacggg tgcggagccc taccgctatc
    13861 tcgtcgaaag catccgcaag ttcccggcgc aggaaccctt tgccgcgatg atccgagagg
    13921 ccggtttcga gcgggtgacg tggcgtaacc tgactggagg catcgcggcc atccactcgg
    13981 gctggcggat ctgacccacg acatggcaag cgccccgatg aatctcatcc gcctggcccg
    14041 cgccggcttc gtgctggcgc agcacggcgt gcagtttgtg ccgcccggca tgaaggcgcc
    14101 cctggcgctc aagctcgcgc acgcgctgac gtggccggtg cgcatgatct cggcaccatt
    14161 ccaggccggc aaaccgcgcg acaggcgcat cacggacgca ctcgcctcct tggggccgag
    14221 ctacatcaag ctgggccaat ttctggcgac gcgcgccgac ctgatcggcc ctgagctggc
    14281 gagcgacctg tgtcatttgc aggacaagtt gccgccgttc tcgatggagg aggcacggcg
    14341 cgcggtcgag gaggcgctgg gcggcaagct cgagacgcat ttcgccgagt tcgggccgcc
    14401 ggtggcggcg gcgtcgatcg cgcaggtgca caaggcggtc gtcatcgaga acggcgagcg
    14461 ccgcaaggtg gccgtcaaga tcctgcggcc gggcatcgag cggcggttca agcgcgatct
    14521 cgacagctat tacttcgccg cgcgcgcgat cgagcggttc catccgccct cgcggcgcct
    14581 gcggccggtg gcggtggtgg atacgctggc gcggacgacc cagctcgaga tggaccttcg
    14641 gctcgaggcg gcggcgatct cggaaatggc ggagaacagc gctgctgacg ccgccgagtt
    14701 ccgcgtgcca caggtcgatt ggcggcgcac cgccaagcgt gtgctgacgg tcgagtggat
    14761 cgacggcatc ccgatctcgg atcgcgtggc gctgcaggcc gcgggccacg acctcggcca
    14821 gctcgggcag atcgtgctgc gcacgtttct caggcatgcg atgcgtgatg gctttttcca
    14881 cgcggacatg catcagggca acctgttcgt cgacgagatg ggccgcatcg ttgccgtcga
    14941 cttcggcatc atgggccggc tcgggatgaa ggagcggcgg ttcctggccg agatcctgca
    15001 cggcatcatc aaccgcgact acaagcgcgc ggcagatgtg catttctggg ccgggtacgt
    15061 gccgccgcat catccggtgg ccgtgttcgc gcaggccttg agggccatcg gcgagccgat
    15121 ccatggccgc accgcctccg aaatctcgat ggccgacctg ctgggccagc tcttcgccta
    15181 caccgaggtc ttcgacatgc agacccggcc cgagctgatc ctgctgcaga agagcatggt
    15241 gatcgtcgag ggcgtggcgc gcgggctcga tcccaacctc aacctgtgga cggcggcaga
    15301 gcccgtcgcg cgggaatggg tcgaggcaaa ctacggcgtc agcggacggt tgcgcgaggc
    15361 gggggaaggc gccgaggtgg tgggcaagat cttgtccgac ctgccgaacc tgctggagcg
    15421 cgtcgagcag ggcgccgcgg cgttcgcgga gatggggagg aacgggattc ggctcgatgt
    15481 cgagaccgtc gagcggatcg gcgcggcaga agcgcgccac acccgctggg gccgcgtggc
    15541 cctttgggtc ggggcgctca gtttggtggc gatggcggtg gtgatgatcg cggggtaggt
    15601 cgggcgactg ttaagtcatc ccggcgctcg tcgccgggat ccaatcagca acttgtgcaa
    15661 gtgtttgctg ttaggtggat cccgggcaca aggcccggga tgaccggctc gaaatcggct
    15721 gcggggcgag ctgcggcaga tgctggccga cgacaagaaa acaaagcagc actcgacaac
    15781 acacggaaat ggtcgccaga ggcgcggtcg accacacgcc tcttcgaggc ctggtttcag
    15841 cagaagctgt tcgatgaccg tctgtccata agactgggcc agctcagcgc cgatacggag
    15901 ttcatgatca gcccgtcggc gacgttgttc gtcaacagca cgttcggctt tccgcgctcg
    15961 ccttcggaaa tggaattccg gagcggaggc ctggaaactg gggagtcttc ggcatcatcg
    16021 accagcagat atggcgtatg cccggttccg atgaggacta gggtgtgaac gtattcgccc
    16081 ggctctccgc aagtccgccc gaccgcaacc agatctcttt caccttcgac ggtgggatca
    16141 ccttcgacgg tgtcatccca gggcggccag acgacgtgct cggagtgggg ttcagctaca
    16201 gccgcatctc tccaagtctg agcgcgctcg atcgggacac gctgcgggcc ggcacctaca
    16261 ctctcgtcca ggattacgag gcgcttatcg aattcagcta ccgcgcccag atcgttccag
    16321 ggtggacgat ccagccggat gtccaataca tgtggcaccc aggcggccat gtgccgatca
    16381 acagcgctcc cggggcaccg gccattccca gcgcgacgat cgtcggggtt cgaacggcca
    16441 tcaattatta ggtcgcgctg atccgagagt tctgtgcgga cctcgccgcg aggtgatgat
    16501 gccggcggcg actgcccagt gctgccgcat ccgctattct cgccttcgca gaaatgggct
    16561 atgcgcagac gattggttcg gcacccacct acgtttccaa cgatcaagga cagccagaaa
    16621 tgtcggagaa aacgctgagg cagcgggcca tgcagggcga ccaggtgctg ggcgccatgg
    16681 tgttcgagtt cttctcgccg ggcatcgccc agatcctgaa gctcgccggc tgcgagttcg
    16741 tgctctacga catggagcac accggcatga g
//
LOCUS       DASVDU010000137        27142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_201927, whole genome shotgun sequence.
ACCESSION   DASVDU010000137 DASVDU010000000
VERSION     DASVDU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 27142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27142
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201927"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF226_08690"
     CDS             complement(<1..218)
                     /locus_tag="VF226_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074102.1"
                     /translation="MSGERVRPAAPGSVKSAGLGLVAAFTATTFLSALLLFSIQPLFA
                     KMVLPVLGGAPSVWAVALCFFQGALLVG"
     gene            complement(316..1005)
                     /locus_tag="VF226_08695"
     CDS             complement(316..1005)
                     /locus_tag="VF226_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7074103.1"
                     /translation="MKITWFGHSCFRIETGSSVLLIDPFLKGNPSFEKSGISWEDATS
                     GVTHVALTHGHDDHIGDAGEICKVRDATLFAVFELALHVQGKGAQKLEPMNTGGTVRS
                     TDFDLTFTQALHSSSSNGIYLGNPCGIILKTRENKTVYHMGDTELFSGMEHINELYRP
                     SIGIVPIGDRFTMGARTAAYACKKYFQFSTVIPCHYGTFDLLDQTADAFVSEMAGENV
                     LVPRIGDPISV"
     gene            1157..1615
                     /gene="ruvX"
                     /locus_tag="VF226_08700"
     CDS             1157..1615
                     /gene="ruvX"
                     /locus_tag="VF226_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7074104.1"
                     /translation="MSRLEQGKRLLGLDLGTKTIGVALSDVTRTIASSFETITRTRFK
                     ADAARLLDIAAKHDVGGFVLGLPRNLDGSEGPRAQATRAFARNLNRLSPLPILLWDER
                     WSTMAAERMLIEADSSRKRRGEVIDKVAATIILQSALDRMKAISTSVESP"
     gene            1697..3340
                     /locus_tag="VF226_08705"
     CDS             1697..3340
                     /locus_tag="VF226_08705"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX7074105.1"
                     /translation="MTDEVPNQSPPFTDINLFTTDLPLQEAVEREGADYAADEITAFG
                     ALCGSAEALERGRLANEFPPRLRTHDPKGRRLDLVEFHPAYYACMAQSMAAGLHCGSW
                     EGQHQSGGQAGANVARAGAFYMTAQMEAGHCCPLTMTNAAVPTLRLQPELADVWVPRI
                     LSRSYDPAFKPASDKRSVTIGMGMTEKQGGTDVRANTTKAEPTANGGPGGEYVLTGHK
                     WFLSAPMSDAFLVLAQAAGGLSCFLVPRFLPDGTVNALRLQRLKDKLGNRSNASAEVE
                     FHGAHGWLIGEEGRGVPTIIEMVTYTRLDCAVASAGLMRLALAMAVHHARHRAVFGRL
                     LIEQPLMVHVLADLALDVEAAVALVFRLARSFDRLEDERAAAWRRLMTPVTKYWVCKI
                     APAMVGEAMECLGGNGYIEENGIARIFREVPVNSIWEGSGNVMALDVLRVLQREPEVA
                     EIVMEDMAQATNGDRHLQAAHERIAGLLHEPRLLDARARELVETLALTAAGAILRMNA
                     PADVADAFIATRLAGHYHQTYGQGLERASTGAILRRVLP"
     gene            complement(3650..5188)
                     /locus_tag="VF226_08710"
     CDS             complement(3650..5188)
                     /locus_tag="VF226_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX7074106.1"
                     /translation="MTNPRRRTSSYVRGAMLSVAFLLPTLSLVVFGSIWLWQTGYLVP
                     WALGAAVVTAVIYVIQLVGLNRFEKSTAPPTAPEELPDSSWTPQEKVAWDEVIEISRS
                     IVPAELDSAQAVYELGKRTVETVARRMHPGEKDPLLRFTVPEALALVERVSKELRPFF
                     QNNIPLGDQITVGQFIRLYSWRGLIDYAERAYDLWRIVRMLNPVTAATHEIRERFSRQ
                     LYEIGREHLIRMLAERFVEEVGRAAIDLYGGRLRLTAEELERRVTPETERDRLEMAAR
                     TAEPLRLLVAGQVNAGKSSLVNALSEQVHGAVDTLPATPAFQAYELRREGLPAALIID
                     SPGLQGGADEIARLIEAASQSDLILWVSDASRADRENDRAALEGVRRYFAEHPNRRRP
                     PMMLVLSHVDRLRPFQDWSPPYDIEKAERPKAKSIRAAMEAVGADLGFAMSDIIPACL
                     NEEVGLYNVDAIWSRIAALLPEAQRANLVRRLGGVQGSFDWGRLWKQAINAGRVISRV
                     VVRS"
     gene            complement(5185..6432)
                     /locus_tag="VF226_08715"
     CDS             complement(5185..6432)
                     /locus_tag="VF226_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX7074107.1"
                     /translation="MIEWPSRLTGVVTRQWNELWAAVMRPQAGAREAEAVASSARQSA
                     TVVWLVGKVQSGKTSIVRTLTGCTDAEIGTGYTPCTRTSRVFDFPPDAPVIRFLDTRG
                     LGEASYEPSEDVAFAETQAHLLIAVMKAMDPQQDQIVSLVGKVRSRHPGWPVVVAQTS
                     LHEGYRPGANHPAGYPFDAEGRPQTEGVPPELARSLIFQRHLFDDLPGTGPVSFVPID
                     FTQPEDGFQPVNYGFDAFVSALQVAAPAGLVAVLENVQAAANDARAKRAYPHVIGYAT
                     AAAAADVVPLAGLVAVPGVQAKMLHSLAAIYGVAWDKRTLGEFASCFGTGTILRAASQ
                     LGIRQLVKFIPVYGQTAGAVAASAASFATTFALGKAACYFLARRRKGEIDVAGVAAAY
                     SAALAEAFNISPSPEPAGQAGSS"
     gene            6650..8257
                     /locus_tag="VF226_08720"
     CDS             6650..8257
                     /locus_tag="VF226_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7074108.1"
                     /translation="MPKIVSQINTSTRAFRANASAMQALVADLAEKRAEAAKGGPEKL
                     RQRHIARGKLLPRDRVMRLIDPGTPFLELSQLAAYGMYSGDIHGAGIITGIGRVAGRE
                     CMIVANDATIKGGTYFPMTVKKHIRAQEIARENRLPCIYLVDSGGANLPHHTEVFPDK
                     EHFGRIFFNQARMSAEGIPQIAVVMGSCTAGGAYVPAMSDESIIVRRQGTIFLGGPPL
                     VKAATGETVSAEDLGGADVHTRLSGVADHFALDDGHALEIARGIVANLNTKKPVDTPI
                     TEPRPPAYPAEELDGVVPASLATQYDPREVIARLVDASEFDEFKARYGTTIVTGFARI
                     EGMPVAILANHGILYSESAQKAAHFIELACQRRIPLLFLQNITGFMVGREAEAGGIAR
                     HGAKMVTAVACADVPKITVIIGGSYGAGNYAMCGRAYGPRFLFMWPNARISVMGGQQA
                     ASVLATVRRENIEAEGKTWSAEEEAAFKAPILEQYERDGHPYYATARLWDDGIITPQE
                     TRRVLALALSACLNAPIPETRFGVFRM"
     gene            complement(8668..9714)
                     /locus_tag="VF226_08725"
     CDS             complement(8668..9714)
                     /locus_tag="VF226_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX7074109.1"
                     /translation="MTATEVIVRAALLSSFRDTVAERGIDVDELLLEAGLDRGALDDP
                     SSELPVDSVALLLELAAERANDPGLGLTLAEASPVGVTGLYGYLVLHASTVRECLQAM
                     TRFAGLLFHPSPMVYREEANFATLSWSFPSRQRVRRVQLASFSNALIMLRLRRVAGRD
                     WTPPFVEVEHPELTCPARFYRIFGPRITFNARENAIAVDKPTLQKANEGADPRLFQIL
                     QETGEAKLSEFAGRPDLIGRTARAIMEVLSDDTPLLERVAEHMRITPRSLQNKLAQQG
                     TTFERVLSDTRRNLAERYLLDTNLPLTEIALLLGFSEQSAFTRAAKGWFGMPPRQLRK
                     EGGWAARNGDGRKS"
     gene            9927..10439
                     /locus_tag="VF226_08730"
     CDS             9927..10439
                     /locus_tag="VF226_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7074110.1"
                     /translation="MSRTQDLSLMGSALHLLHRAGQRADELFSATMGDNELTPRQFAV
                     LKAVARMENPSQTALVHDTGIDRSTIADIVRRLIKKGLLQRRRTSHDARMYAVKLTAA
                     GRELLEAAEPAARTTEEKLLSVLPAQQRNAMIEALSTIVTVLDSSANGHANGNGAAPA
                     PKGRRSRARN"
     gene            10618..11067
                     /locus_tag="VF226_08735"
     CDS             10618..11067
                     /locus_tag="VF226_08735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX7074111.1"
                     /translation="MTEVLFYHLERQPLERVLPSLLERTLERGWRAVVQCGSEERLEA
                     LDTWLWTYRDDSFLPHGTSRDGPGSMQPIFLSTGDDNPNGACVRFLVEGAVISDFSGY
                     ERVVHIFDGHDETAVEQARAEWKRARAAGCEVTYWQQADNGRWEKRA"
     gene            complement(11098..11463)
                     /locus_tag="VF226_08740"
     CDS             complement(11098..11463)
                     /locus_tag="VF226_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074112.1"
                     /translation="MAEQRLTSKAFLLFGGLLIWAAHFMLVYSVNAVVCARGLQDLRV
                     LGAGLIPFTVAIATLVALAAASRLLWVGSGWLGPLRGEPHDDRASAFLRQVSIALALL
                     SMVAIFLTALPSFVVVPCG"
     gene            complement(11469..14033)
                     /locus_tag="VF226_08745"
     CDS             complement(11469..14033)
                     /locus_tag="VF226_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7074113.1"
                     /translation="MTVVGDRSEERTAERKPANRFPNEVPRPEGELNELRKPWVPPRG
                     WRILSAVNNTYVGLAYIATGLIIFILAGVLAVLMRTQLATPENDLIDYALYNQLFTMH
                     GTMMMFLFAVPIVEAFAVLLMPNILGTRELPFPRLSAYAFWIYAFGSLAFFFTLFFGV
                     APDTGWFMYTPLSDARYSPGINADMWLLGIGFIEIAAIAGAIEIIVGFAKTRAPGMSI
                     DRIPVFAWAMLIFALMIVFAFPALILGTILLEIERALGWPFFMAEKGGDPLLWQHLFW
                     FFGHPEVYIIFIPAAGMVSTILPAMAATPLVGHRLVVLALITMGVISFGLWVHHMYAT
                     GLPQLSLGFFSAASMSVAIASGIQVFAWIATIASGRLKLKTPALFIVGFLIVFTMGGL
                     TGVMVASVPFDLQVHDTYFVVAHFHYVLVGGMVLPVLAAIYYWYPTVSQKQLSERVGR
                     WVFGLFFIGLNATFFTMHITGLMGMPRRVYTYLAVMDWGALNLISTFGAYMMAAAIAL
                     LVFDIVRTFRFSFSDTVGNIWDAGTLEWMRTYTYGTRSIPEVDSRYPLWTRPELSDET
                     DEGRYYLPGTLTGRREALVTSPIDARPVYVMQVPGPHWGPFLAGIATTIFFYLLTFKL
                     VLPAVFFGVVAVALILWWVWDLDHGPVRRPAHIGGGLVLPVYTSGSVNHSWWGTVTLL
                     LVQGAFFTCLVFSYFFLWLVNPHSWPPPGVSMPSLAWPAVAAALYAASSAAIFFAGRA
                     LARPSSGALQLLFPSALLLFAAAVGVQVYSEWQTGLRPTQHSYGAMVYLFGALQGFLG
                     GVLLVMAGFTMARSLAGKLDAVRRVTFDNTMLLWHYATAQGLVAVGIVSLFPRALG"
     gene            complement(14030..15031)
                     /gene="coxB"
                     /locus_tag="VF226_08750"
     CDS             complement(14030..15031)
                     /gene="coxB"
                     /locus_tag="VF226_08750"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7074114.1"
                     /translation="MQRRVPDLLPPLASGAGCLLLTGCNWSQSVLAPRGSHAEAIDGI
                     LRVLFIGGALIFSLVAVLLATAILLRPSLRAWMASNNFIIGFGIVFPVITLMALLTFS
                     SVVSRTMNGISDPKIRIEVIGEQFWWRVNYLDGQGNPALATANEIRIPVNTPVEFILK
                     TQDVIHSFWVPSLAGKLDMIPGRTNRYTFAATSPGVYRGQCAEYCGDQHALMAFYVVA
                     VPQADYESWFAEQVGNAAEPTTPLLRQGRDLFLAAGCGSCHTIRGTPADGTLGPDLTR
                     VGSRLSIAAGTYPVNVGTLAGWTSSAPHLKPGNLMPSFGNLGGEQLRAIAAYMESLK"
     gene            complement(15229..15891)
                     /locus_tag="VF226_08755"
     CDS             complement(15229..15891)
                     /locus_tag="VF226_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074115.1"
                     /translation="MALVGSGVLGVWHGVAPGMERMFDDWYNAEHHAERIGIDGFLRG
                     RRYINLRHGRRYFCRYDVEDVSVLASPAYLAALNNPTERSRRKLPHFRSTLRGAYRVT
                     KRHGWADGGYAVVCRFGQAGDDGADPGPAIEEFTAHVLDKPAVTAVEYWEVEPEVTNI
                     ASEEKFLRKALDIFPARVLVIDGSDPDAMEAAIEAAPHRHALDGAEMDFCKLVYHAAK
                     RA"
     gene            16163..17953
                     /locus_tag="VF226_08760"
     CDS             16163..17953
                     /locus_tag="VF226_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7074116.1"
                     /translation="MPRKKGAELITEFLVREKIPYVFGICGHGNVGLLDELYNVRNEV
                     KLVSPRHEQTAGHMADAYFRVKHQPVATLTSCGPGSANLVMALAVAQTDSSAFLAITA
                     NVPTSQFNRSPFQELNRHYQADFPNVVRPVVKRVFQPTRVEQLPLMLRQAATTMTSGR
                     PGPVSLDIPFNVFQEEADVENEAPWNGFNLRRSGASPEDVSTAVDMMLKAVRPVLFVG
                     HGVTLSEAGAELTELAHRLQIPVISSPNGMGTIDMSDLLSLGFIGRNGAYPANQAGRH
                     ADLVVAIGARFDDRSASSWLPGYSWNFPKTKLVHVDVDHAELGRNYPPDLGILADART
                     FLRQVLAEIERRNPPRTGRLEAWHAQIATWRKQWDDYVRPNFELHTSPLRPERVVADL
                     RAVLPDDAIISLDSGVHHNWFMQFWEARRPQTMLNTWGFSGMGFGPSGILGAKLAAPD
                     RPCVSVCGDGGFTMVPHVLCTAVEYDIPAVWVVWNNFSWSAIRDIQYGLFSGREIGTA
                     FYQGPNNKPYNPDFAAWARACGVEGITVTKSEDLKGALEHAIKLNKPVLLDVHVDAEV
                     RPPATGTWALPPIPHKEPVFGKPYVPGAGE"
     gene            17974..18918
                     /locus_tag="VF226_08765"
     CDS             17974..18918
                     /locus_tag="VF226_08765"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074117.1"
                     /translation="MRVLVTGASGFIGYAVTEALAARGDEVLATDQVIGPRLSALAEA
                     NGKVKAIPGEITEWPHLVGLIKDGKPEKVVHCAAIVGVPASVGSPINTFRVNIEGTIH
                     LLEALRLFGVRRMVNISSEEIYGAFQADVIDEEHPCRPTKPYGISKFVVEQMSRDYAA
                     AYGLECIHTRTCWVYGPYYPRQRAPRNLLEAAIAGRPLHLPGGQSFSVDQVHISDLVQ
                     GVLLALDKPQHRYDAYHIATGVSTPLSHVVDLIKERLPKADISVGPGPLTFVDGTEVV
                     RKGALSIARAQKELGYSPRYDMRTGINNWIDLLLTGKG"
     gene            complement(19214..20623)
                     /locus_tag="VF226_08770"
     CDS             complement(19214..20623)
                     /locus_tag="VF226_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX7074118.1"
                     /translation="MNGVQLGVIAYVVVQFAIGIWVARRVKTTTDFLIAGRTLGLGLV
                     SFSVFATFFGAEAIVGTSGAVYEEGLVGGQIDPFGYALAIFLVGAIFAVPLWRRGIVT
                     FADFFRERYSSAVEKLVVLVLLPGSMFWAAAQVRAFGQVVSAASGLDLSMAIGIAAVL
                     VVGYSVLGGLLADAWTDFIQGLAIVAGLVVLAVVVAGQIGGVAEGLAKVEPERMQALT
                     IGEDGLLTFIESFAVPICGTVVAIEIISRVLGARTAGVAARGAMLGGIIYLVVGAIPV
                     FLGLVGPQLLPDVDDAEQIVPLLAEAHLPGLLYVLFAGAVISAILSTVDSVLLASSGH
                     VSHNIVERLMPWLDDRSRLMLNRAILAGLAVFAFAMAISAERVKDLVETASAAGSAGV
                     FVVAVFGLFTRFGGPVAAFAAITVGALSWLVFGALELETPYIAALGLALFTYLLAAVV
                     ENPAEALSQAGARACKDGD"
     gene            20841..22643
                     /locus_tag="VF226_08775"
     CDS             20841..22643
                     /locus_tag="VF226_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074119.1"
                     /translation="MPAALLRPTIAALTAFAVAAAPMSPPARASVKLTRADYEACQAG
                     DEQTFRSAVERITLNALQNGLKSIDFKAVVAAEWRRVGLDEIIDKQVDVAIAEVNDES
                     SWGDLIQSLAYKEKSQELATAVAERVYRSEPVKAAIEQLAGGVGKDVGRNIELATIDA
                     AEPSLQCLQAFLGPRFGTTVSRVVASDAGKEFAIDAETARAQVSTTAVLIEGSEGIAG
                     AVILLVRRQLSRMAARIGHRIVGAVLGRLVSIVAGGVGLVLIAKDIWELRSGVLPIIA
                     EEMKSPATKQRVREELASSIAGQLDEQVQDISAQAADGIVEIWREFRRAHAKVLELAE
                     QNEPFRSFLDAAHPRQLPIIDELVGLIAANEGDPGVFKRLQDGTLYSAVNNLTEPGMT
                     IARETRSVDAALRWTAIAGDQLDRVTEYELHKRASADDFTKATLSRLFALDDRVATVR
                     LASIDRASRDVLFDLQNDDLRRLARGLTEPELTTLARYLTGLRKSASERVLRAVAQAP
                     AKMQVLASARVRDAVIASRDQDAAVAMMLRTGSLPNPALVSSDFGLVLDGQVSPVLLW
                     EKHSVLLIVMSVLAFGGLLFLKRLLFGRRRRAAA"
     gene            22663..22977
                     /locus_tag="VF226_08780"
     CDS             22663..22977
                     /locus_tag="VF226_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX7074120.1"
                     /translation="MRLVTTETIDEPVRHGEMIYACAVSGANVLRDMREAITNTIGGE
                     MTRYEALLDKTIKRALANLAERAREKGYDGVLAVRISHPVITNGAVEVVVTGTGYWRA
                     AD"
     gene            complement(23184..24500)
                     /locus_tag="VF226_08785"
     CDS             complement(23184..24500)
                     /locus_tag="VF226_08785"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7074121.1"
                     /translation="MEQPNTRPRAVVVGGGAGGLLLAIRLAGLRRGKLLDVALVDKSQ
                     THVWKPLLHEVASGSLYPSTDEIDYFSLARWHGFTFHHGAFEGLDRENRLIHVAAVHD
                     RDRMIIPPRKVPYDFAIMALGSVTNDFGIPGVKEHTYSLDNVEEAAEFHRTLVNACLQ
                     ANYAEPERRPEYLNVIIVGGGATGVELAAELRSSIRVITQFRLENLDADSFVRLTILN
                     LDPRVLVQLPERISDAITEELGNLGISVRNSEQVVRVDQTHIETKSGNRYPADLAVWA
                     AGVKAPDCLRNVAGLETDRLDRLVVTETLQTTRDPRVLAMGDCAAAPWSGKNGKVPPR
                     AQAANQQARYIAKAIPRILAEQPLKPFRYNDFGSLVSLGEENAIGTLMGYVSGPRLRV
                     EGFIARLIYRWLYKRHQAALYGWSLAILATIGNWLRGTTRPRVKLH"
     gene            24693..25331
                     /locus_tag="VF226_08790"
     CDS             24693..25331
                     /locus_tag="VF226_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7074122.1"
                     /translation="MLRVEKLKVGDLPPLTFEVAASECLSVEGPSGSGKTRLLRAIAD
                     LDDAEGYVFLDGVERRELAAPEWRRRVRYFAAEPGWWAATARDHFPSDVDPARLDRLL
                     DALGLGAGLLDQPIGQLSTGERLRMALVRGLIDEPRVLLLDEPTGALDAQSRALVDEL
                     IRFQLLAGRIVLLVSHNAAEIDRLAHQRLQLKALAPRPAVPEQPLRDGSAAR"
     gene            25328..26125
                     /gene="fetB"
                     /locus_tag="VF226_08795"
     CDS             25328..26125
                     /gene="fetB"
                     /locus_tag="VF226_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX7074123.1"
                     /translation="MTYVPLTWIDLAAAAVLIVFNGALSWALRLGLERSIALASVRMV
                     VQLGAVGFVLKFIFEQTSPLLTAAMALVMVAVAGHEVLARQETRIGGWMAYGIGTGTL
                     FFVGLVATIYSMIVIIGPEPWYAPRYVLPILGMVLGNTLTGVSLVLNTITQTAKRERA
                     AIEARLALGETRFGATSSVVVHGVKTGLMPILNAMAATGLVTLPGMMTGQILVGMDPV
                     EAAKYQIMIMFVIAASTAFGVMLAAIGGAWLITDARHRLRVAALGLV"
     gene            complement(26157..26477)
                     /locus_tag="VF226_08800"
     CDS             complement(26157..26477)
                     /locus_tag="VF226_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074124.1"
                     /translation="MGSRIPFVAALASALLLTQISPVEAACQKLGFSVNDYGKEGPTR
                     DAKDLLDKYIAQWTAERGIKKYTVSKKTVSCELFLDFIVFDEHTCSAEATVCWSGPMP
                     AKAN"
     gene            complement(26660..27010)
                     /locus_tag="VF226_08805"
     CDS             complement(26660..27010)
                     /locus_tag="VF226_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074125.1"
                     /translation="MKRIFAFGVVFCAAVPWLMSGPAAHANGSHVKRGQVTSHRVVKP
                     RAYGYVVWPGGGYSYTFRDVINTYRSPRTKWGSTNFYRNWRVDRQTEFGPFDHGFFFD
                     SPVAPNAGYAPYQH"
BASE COUNT         5099 a         8623 c         8772 g         4648 t
ORIGIN      
        1 tagccgacga gcagcgcacc ttggaaaaag cacagcgcga ccgcccaaac cgacggcgcg
       61 ccgccgagaa ccggaagcac cattttcgcg aagagcggct ggatcgagaa cagcagaagc
      121 gccgacaaaa aggtcgtggc cgtgaaggcg gcgaccagcc ccagccctgc ggacttgaca
      181 ctgcccggcg cggccggccg cacgcgttca ccgctcattc actctcctgc cgaacgccgg
      241 ctgcatggtg cagcggcctt agctcgcgaa actggcgcct gcaaccggca tatctcttcg
      301 cagagggggt ccgcctcaca ccgagatcgg atcgcctatc ctcggcacca gaacgttttc
      361 acccgccatt tcgctcacga aggcatcggc agtctggtcg agcagatcga acgtcccgta
      421 gtggcagggg atcaccgtgc tgaactgaaa atacttcttg cacgcataag ccgccgtgcg
      481 cgcgcccatg gtgaaacggt caccaatggg gacgatgccg atgctcggac gatagagctc
      541 gttgatatgc tccatgccgg agaacagctc cgtgtctccc atgtggtaaa cggtcttgtt
      601 ctcccgcgtc ttcaggatga tgccacacgg attaccgaga taaatcccat ttgacgagga
      661 ggagtgcagc gcctgcgtaa acgtcaggtc gaagtccgtc gaacggaccg ttccacccgt
      721 gttcatgggc tcgagcttct gggcgccctt gccctgtacg tgcaaggcca gctcgaatac
      781 ggcgaagagc gttgcatcgc gcactttgca gatctcgccg gcgtcaccga tgtggtcgtc
      841 atggccatgg gtgagcgcca catgggtcac tccgctggtt gcatcctccc aggagatgcc
      901 agacttctcg aatgacgggt tgcccttcag gaagggatcg atcagaagga cgctgctgcc
      961 ggtctcgatg cgaaagcagg aatggccgaa ccaggtgatc ttcattggca atttcctcgt
     1021 ttctggggca acgatctgcg atccggcgca ctataggacg gctacgaatt gcaacaagcc
     1081 tctctcgacc agatgtgagc ccgacatcgt gcctgacagc cttcccggcg aaacactgac
     1141 cgagcccgct gatttcatgt cgcggctgga gcaaggcaag cgcctcctcg gcctcgacct
     1201 cggcacgaaa acgatcgggg tggcgctgtc ggacgtgacc cgcaccatcg cgtcgtcctt
     1261 cgagacgatt acccggacca ggttcaaggc cgatgccgcg cgcctgctgg acatcgcagc
     1321 caagcacgat gtcggcggct tcgtccttgg tttgccccgc aatctcgacg gcagcgaggg
     1381 gccgcgtgcc caggcgaccc gtgcgttcgc gcgcaacctc aatcggctgt cgccgttacc
     1441 gatcctgctt tgggacgaac gctggtcgac gatggcggcc gagcggatgc tgattgaggc
     1501 ggacagcagc cgcaagcggc ggggcgaagt catcgacaag gtggccgcga caatcatcct
     1561 gcagtcggcg ctggacagga tgaaggccat cagcacctcc gtcgaatcgc catagacttg
     1621 gcaagctcgc cgcgtggtga gagcttattt gaagtccaga ggtgaagccc tcgcatattg
     1681 aattcggacc gttgtgatga cagacgaggt cccaaaccag tctccgcctt ttaccgacat
     1741 caatctgttc acgacggact tgccgctgca ggaggccgtg gaacgggaag gggctgatta
     1801 cgcggcggat gaaattactg cctttggtgc gctctgtggc agcgccgagg ctctggaacg
     1861 ggggcggctc gccaatgagt ttccgccgcg gctgaggacg cacgatccga agggccgccg
     1921 gcttgatctg gtcgagttcc atccggccta ttacgcctgc atggcgcaga gcatggccgc
     1981 cggccttcac tgtggctcct gggaggggca gcatcagtcg ggcggtcagg ccggcgccaa
     2041 cgtcgcccgc gcaggcgcct tctacatgac cgcgcagatg gaggcgggcc actgctgccc
     2101 actgaccatg acgaacgcgg cggtgccgac gcttcgcctg cagcccgagc tggcggacgt
     2161 atgggtgccg cgcatcctgt cacgcagcta cgatcctgct ttcaagccgg ccagtgacaa
     2221 gcgctctgtc acgatcggca tgggcatgac ggagaagcag ggcgggaccg acgttcgcgc
     2281 caacacgacc aaagccgagc caacggcaaa tggcgggccg ggcggggagt acgttctcac
     2341 cggccacaag tggttcctct cggcgccgat gtccgatgcc ttcctcgtgc tggcacaagc
     2401 ggcgggcggt ctctcctgct ttctcgtgcc gcggtttctg ccggacggga cggtgaatgc
     2461 gctgaggctg cagcggctca aggataagct cggcaaccgg tcgaatgctt ccgccgaagt
     2521 ggagttccat ggcgcgcacg gctggctcat cggcgaggaa ggccgcggcg tgccgacgat
     2581 catcgagatg gtgacctaca cgcggctcga ctgcgccgtg gcatccgccg gcctcatgcg
     2641 gctggcgctg gccatggccg tccaccacgc gcgccatcgg gcggtcttcg gccggctgct
     2701 gatcgagcag ccgctgatgg tgcatgtgct ggcggacctg gcgctcgatg tcgaggcggc
     2761 cgtcgcgctc gtcttccggc tcgcccgcag cttcgatcgc ctcgaggatg agcgcgctgc
     2821 ggcctggcgg cggctcatga ccccggtgac caaatactgg gtctgcaaga tcgcgccggc
     2881 catggtcggc gaggcgatgg agtgcctggg tggcaacggc tacatcgagg aaaacgggat
     2941 cgcccgcatc tttcgcgagg tgccggtcaa cagcatctgg gagggctccg gcaacgtcat
     3001 ggcgctcgac gtcttgcgcg tcctgcaaag ggagcccgag gtggccgaga tcgtgatgga
     3061 ggacatggcg caggcaacga acggcgaccg acacctccag gctgcgcacg aacgcatcgc
     3121 aggcctgctg cacgagcctc ggctgctcga cgcgcgcgcc cgcgagctcg tggaaaccct
     3181 ggcgctcacc gcggctggcg caattctccg catgaatgca ccggccgatg tcgccgatgc
     3241 cttcattgcg acccgtcttg ccgggcacta ccaccagacc tacggccaag gactggagcg
     3301 ggcgagcacg ggcgcaatcc tgcgtcgggt cttgccctga cacagatggt ccgcggtccg
     3361 caatggagca gatcccatcc gggtgggatc acctggtggg agaagatgct cgcagaagaa
     3421 gaatctcaaa ttttcacggg agctcatgcg agcggaacct gcgctggccg tgtggtgagg
     3481 gcctgtccga caaaaaaccg gcgtcatccc ggtgcttgtc accgggatcc agcgtcccgc
     3541 tggctccacg gcttgtggca agatggatcc cgggcgcaag gcccgggatg actcggggat
     3601 tgcgagttgc tgcgcagatc ttccagctcc cgcctgaaat acagtgcgct cagctccgaa
     3661 ccaccacccg cgagatcacg cggccggcgt tgatggcctg cttccagagg cggccccagt
     3721 cgaaactgcc ctggacgccg ccgagccggc ggacgaggtt ggcgcgctgc gcttcgggca
     3781 gcaaggcggc aatccggctc cagatggcgt ccacgttata gagcccgacc tcctcgttga
     3841 ggcaggcggg aatgatgtcg ctcatggcaa agccgaggtc ggctccaacg gcttccatgg
     3901 cagcgcggat cgacttggcc ttcggccgct cggccttctc gatgtcgtag ggcggcgacc
     3961 aatcctggaa cggacgcagc cggtcgacat ggctcagcac cagcatcatc ggcgggcggc
     4021 gacggttcgg gtgctcggcg aagtagcggc gtacgccttc cagagcggcg cggtcgttct
     4081 cgcgatccgc gcggcttgca tcggagaccc agagaatgag gtcgctttgg gaagccgcct
     4141 cgatcagccg ggcgatttcg tcagcaccgc cctgcaggcc ggggctgtcg atgatgagtg
     4201 ctgcgggaag gccttcgcgt cgtagctcgt aggcctggaa ggccggagtc gccggcagcg
     4261 tgtccactgc tccgtgcacc tgctccgaca gcgcgttgac gaggctcgac ttccccgcat
     4321 tcacttgccc cgcaacgagc agccgcagcg gctccgccgt ccgggcagcc atctccaggc
     4381 ggtcgcgctc ggtctcgggc gtgacgcgcc gctccagctc ctccgccgtc agccgcagcc
     4441 ggccgccata gaggtcgatg gctgcgcgcc ccacctcctc gacgaagcgc tccgccagca
     4501 tccgtatgag gtgctcgcga ccgatctcgt aaagctggcg agagaaccgc tcgcggatct
     4561 cgtgcgtcgc ggcggttacc gggttgagca tgcgcacgat gcgccacagg tcgtaagcgc
     4621 gttcggcgta atcgatcagc ccgcgccagc tgtagagcct gatgaactgg ccgaccgtga
     4681 tctgatctcc gagcgggatg ttgttctgga agaacggcct gagctccttg ctcacccgct
     4741 cgaccagggc caaggcttcc ggaaccgtga accgcagcag cggatccttc tcgcccgggt
     4801 gcatacgcct ggccaccgtc tcgacagtgc gcttgccgag ctcgtagacg gcctgggcgc
     4861 tgtcgagctc agccggcacg atgctccgtg agatctcgat gacctcgtcc caggcgacct
     4921 tttcctgggg agtccagctc gagtcaggca actcttccgg cgcggttggg ggcgcggtcg
     4981 acttctcgaa ccgattgagc cccacgagct ggatgacgta gatgacagcc gtcaccaccg
     5041 ccgccccgag cgcccagggc acgagatacc cggtctgcca cagccagatg ctgccgaaaa
     5101 cgacgaggga gagcgtcggc agcaggaagg ccaccgagag catcgcgccg cggacgtatg
     5161 agctggttcg gcggcgcgga ttggtcatga cgaaccggcc tgccccgcgg gttccggcga
     5221 cggtgagatg ttgaacgctt cggcaagcgc ggcgctatag gcggctgcga ccccggccac
     5281 gtcgatttct cctttccgtc ggcgggcgag aaaatagcac gccgccttgc cgagcgcgaa
     5341 agtcgtcgcg aagcttgctg cgctggctgc gacggcgccc gcagtctggc catagaccgg
     5401 gatgaacttg acgagctgcc ggattccgag ctggctggcg gcgcgcaaga tggtgccggt
     5461 cccaaaacag ctcgcgaact cgccgagcgt ccgcttgtcc caggcaacgc catagattgc
     5521 ggccaggcta tgcagcattt tggcctgcac ccccggcacc gcgacgaggc ctgcaagcgg
     5581 tacgacgtcg gcggccgcgg ccgcggtggc gtagccgatg acatgggggt aggcgcgttt
     5641 tgcgcgggcg tcgttggccg cagcctgcac gttttcgagc acggccacca aaccggctgg
     5701 cgccgcgacc tgaagcgccg agacgaaggc gtcgaacccg tagttgacgg gctggaatcc
     5761 gtcttccggc tgcgtgaagt cgatcgggac gaatgaaaca gggccggtcc ccggcaggtc
     5821 gtcgaacaag tgtcgctgaa agatcagact gcgcgccagc tcgggcggga ctccttcggt
     5881 ctgagggcgc ccctcggcat cgaacggata cccggcgggg tggtttgcgc ccgggcgata
     5941 tccttcatgc agggaagtct gtgcaacgac aaccggccag cccggatggc ggctgcgcac
     6001 cttccccacc agcgagacga tctggtcctg ctggggatcc atcgccttca tgaccgcgat
     6061 caggaggtgc gcctgtgtct cggcgaacgc cacgtcctcg ctcggctcgt agctggcctc
     6121 gccgagccca cgcgtgtcga ggaaccggat caccggcgcg tcgggaggga aatcgaagac
     6181 gcgtgacgtc cgggtgcagg gggtatagcc cgtgccgatc tcggcatccg tgcagccggt
     6241 gagcgtgcgt acgatcgacg tcttgccgga ctggaccttg cccaccagcc acactaccgt
     6301 cgcactctgc cgcgctgacg aggcaaccgc ttccgcctcg cgcgcgccag cctggggcct
     6361 catcaccgcg gcccacagct cgttccactg ccgggtaacg acacctgtca gccgggacgg
     6421 ccattcgatc attccgctgt gcctcacagt cacgcttttg ctggacctag cgttggagcc
     6481 atcgagcttc aagagctgcc caggaacgcg ccggctcgcc gaaggttggg tgagccgggg
     6541 tgtgctccag cctgaagcgc tgtcggctcg gtcgcacgcg cgacggccga acttgtcgcg
     6601 aggcgcaggc gctgcttgat tggcgcctcg cgtcgacgca gaaagagcca tgcccaagat
     6661 cgtttcccag ataaacacct ccacccgggc tttcagggcc aatgccagcg ccatgcaggc
     6721 cttggtggcg gatctcgccg aaaagcgggc agaggccgcg aagggcggcc ctgagaagct
     6781 gcgtcagcgg catatcgcgc gcggcaagct gttgccccgc gaccgcgtca tgagactgat
     6841 cgaccccggt accccgttcc tggagctgtc ccagctcgct gcctacggca tgtactcggg
     6901 cgacatccac ggcgcgggca tcatcaccgg aattggccgg gttgcggggc gcgagtgcat
     6961 gatcgtcgcc aacgacgcga cgatcaaggg cggcacgtac tttccgatga cggtcaagaa
     7021 gcacatccgc gcgcaggaga tcgcgcgcga gaaccggctc ccgtgcatct atctcgtcga
     7081 cagcggcggc gccaacctgc cgcatcacac ggaggtgttc cccgacaagg agcacttcgg
     7141 ccgcatcttc ttcaaccagg cgcgcatgtc agcggagggc atcccgcaaa tcgccgtcgt
     7201 gatgggaagc tgcactgcag gcggggcata tgtgcccgcc atgtcggatg agagcatcat
     7261 cgtgcgccgt cagggcacga tctttctggg cggcccgccc ctggtgaagg cggcgaccgg
     7321 cgagactgtg agtgcggagg acctcggcgg cgctgatgtc cacacgcggc tgtcgggcgt
     7381 cgccgatcac ttcgcgctcg acgatggcca tgcgctcgag atcgcgcggg gcatcgtcgc
     7441 caatctcaac acgaagaagc cggtcgatac ccccatcacc gagccgaggc cgccggccta
     7501 tcctgccgaa gagctcgacg gggtcgtccc ggcatcgctt gccacccaat acgacccgcg
     7561 cgaagtgatc gcgcggctgg tcgacgcctc cgagttcgac gagttcaagg cgcgctatgg
     7621 caccaccatc gtcaccggct ttgcgcggat cgagggtatg ccggtggcga tcctcgccaa
     7681 tcacggcatc ctttactcgg aatccgcgca gaaggcggcc catttcatcg agctcgcctg
     7741 ccagcggcgc ataccgttgc tattcctgca aaacatcacg gggttcatgg tggggcgcga
     7801 ggcggaggcc ggcggcatag cccggcatgg cgccaagatg gtcacagcgg tggcgtgcgc
     7861 agacgtgccc aagatcacgg tcatcatcgg cggcagctac ggggccggca actacgccat
     7921 gtgcggccgc gcctatggtc cacgtttcct cttcatgtgg ccgaatgcgc gcatctcggt
     7981 gatgggcgga cagcaggccg cgtcggtgct ggcgacagtg cggcgcgaga atatcgaagc
     8041 tgaaggcaag acctggagcg cggaagagga ggccgccttc aaggcgccga ttctcgagca
     8101 gtacgagcgt gacgggcacc catactacgc aaccgcgcgc ctgtgggacg acggcatcat
     8161 cacaccgcaa gagacacgcc gcgtgctggc tctggctttg tccgcctgcc tcaacgcgcc
     8221 gatccccgaa acacggttcg gtgtcttccg catgtgacgc cttaggcctg ttccaattaa
     8281 gattgaccgt tcacggttaa tcgaggcgat tgtggcaagt tgtgcattat gggaagcaca
     8341 atctccggta tatcgaaccc cataggcggg ttcttgatgg aacggaggag ctcccgatgc
     8401 gcgtcttgct agccactatg ctgattgcag ccgccgcagt gctcgcgatg ccgaccccat
     8461 ctgccgaggc ggatggcttc ttcttccgca gtcgtgcccc agtggcggca tactacgctc
     8521 ctgctccgcg gagatactat cgcgtcgtag ctccggcgta ctatgcgcct cctgcccgga
     8581 ggtggtgcct gttcgattgg ttcggctggg gtcggcgcag cggttggtga gccgcctgcc
     8641 gccggagcgg gtgagctgcg accagcgtca ggatttcctg ccgtcaccgt tgcgagccgc
     8701 ccatcctcct tccttgcgca gctgccgcgg cggcataccg aaccagccct tggccgcgcg
     8761 cgtgaacgcg ctttgctcgg aaaagccgag caaaagtgca atttccgtaa gcggcaggtt
     8821 cgtgtccagc agatagcgct ccgcgaggtt gcggcgcgtg tcgctcagga cgcgctcgaa
     8881 ggtcgtcccc tgctgggcca gtttgttctg aaggctgcgc ggtgtgatcc gcatgtgctc
     8941 cgccacgcgc tcgagcaggg gggtgtcgtc gctcaacacc tccatgatgg cgcgtgcggt
     9001 gcgtccgatg agatccggcc tgccggcgaa ctcgctcagc ttggcctcgc cagtctcttg
     9061 cagaatctgg aacaggcgcg gatcggcgcc ctcgttcgcc ttctgcaacg tcggtttgtc
     9121 gacggcgatc gcgttctccc gggcattgaa cgtgatccgt gggccgaaga tgcgataaaa
     9181 ccgcgcaggg caggtcagct ccggatgctc gacctcgacg aaaggcggtg tccagtcgcg
     9241 gccggccact ctcctcaatc tgagcatgat gagtgcattg gagaagctcg ccagttggac
     9301 tcgacgcaca cgctggcgtg aggggaagga ccaggacaat gtcgcgaagt tcgcctcttc
     9361 ccgatagacc attgggctcg gatgaaagag caagcctgcg aagcgcgtca tggcctgcag
     9421 gcattcgcga acggtgctgg cgtgcagcac cagatagccg taaaggcctg tgacgccgac
     9481 gggagaggct tccgccaagg tgagccccag cccgggatcg ttggcccgct cggcagccag
     9541 ctccaacagc aaggcaaccg agtccacggg aagttcgctc gagggatcgt cgagcgctcc
     9601 acgatcgagc cccgcttcga gcaggagttc gtccacgtcg atgccacgct ctgcgacggt
     9661 gtcgcgaaag ctggacagga gggcggcacg gacgattact tctgtcgcag tcatggtccc
     9721 gcgcaacatg gcgcgcaatg gcgaacgacc caatggcgcc aaacgccgcg atcatgtgca
     9781 gctcacgtca aaaacggtgt gccaaaggtc aagtctcgcg cactggaact aacgtaaatg
     9841 cttcgaaaaa tcagaagcat gaccgttgcg gttaagaacg ctcaagtaga gcgctggtca
     9901 ctcacctccc tggagcaatt tcggacatgt cccgaacgca agacctttca ctcatgggct
     9961 cggcactgca cctgcttcac cgcgccggcc aacgtgccga cgagctgttt tcggcaacga
    10021 tgggcgacaa cgagctcaca ccgcgtcagt tcgcggtgct gaaggccgtg gcgagaatgg
    10081 agaatcccag tcagacagcg ctcgttcacg acaccgggat cgatcgctcc acgattgcag
    10141 acatcgtgcg tcgtctcatc aagaagggcc tgctgcagcg gcgccgtacg agccacgatg
    10201 cccggatgta cgcggtgaag ctcaccgctg cgggccgcga acttctcgaa gcggcggaac
    10261 cggccgcccg gacgactgag gaaaagctgc tgtccgtcct tccggcccag caacgcaacg
    10321 cgatgatcga agcgctgagc accatcgtca cggtgctcga ctcgtcggcg aatgggcacg
    10381 ccaacggcaa tggcgccgcg ccggccccga aggggcgccg ctccagggcg cgtaactgag
    10441 gcagtccggc gagtccggat ctcgttccgc tccggtctgc aatgcccgcg ggcatcttcc
    10501 gcgggcattt cctttggcgc cggtgcatac tgctggcgat tcaggtccgg gtcgcccgac
    10561 ggcgcaccga gcccgcgtcc tgtgccggac gtctcaagtc atctggcgga gaggaacgtg
    10621 acagaggtgc tgttctatca tctcgaacga cagccgctcg agcgcgtgct tccgagcctg
    10681 ctggagcgta cgctggagcg tggttggcgg gcggtggtcc aatgcgggag cgaggagcgg
    10741 ctcgaagcgc tggacacctg gctctggacc tatcgcgacg acagcttcct gccgcacggt
    10801 accagccgtg atggacctgg ctcgatgcag ccgattttcc tcagcaccgg cgatgacaac
    10861 ccgaatggcg cgtgtgtgcg cttcctggtg gaaggggcgg tgatctcgga tttctccggc
    10921 tacgaacgcg tggtgcacat cttcgacggt cacgacgaga cagcggtgga gcaagcacgc
    10981 gccgaatgga aacgggcgcg cgccgcgggc tgtgaagtga cctattggca gcaggccgac
    11041 aatggccgtt gggagaaacg ggcctgatcg cgctggctca tggtccctgg ggagaaatca
    11101 tccgcagggc acgacgacga aggagggcag cgcagtcaga aaaatcgcaa ccatcgagag
    11161 cagcgccagc gcgattgaaa cctggcgcag aaaggcactg gcgcggtcgt catgcggctc
    11221 gccgcgcaaa ggcccgagcc agccggagcc gacccacaac agacggctgg cagccgcgag
    11281 ggccacgagc gtggcaatgg ccaccgtgaa cgggatgagg ccagctccga gcacgcgaag
    11341 gtcctgaagg ccgcgcgcac acacgaccgc gttgacggaa tagacgagca tgaagtgggc
    11401 ggcccagatg agcagccctc cgaaaaggag gaaggccttc gacgtgaggc gctgctcggc
    11461 cacggcggtc acccgagcgc ccggggaaag aggctgacga tcccgacagc gacgagtccc
    11521 tgcgccgtcg cgtagtgcca gagcagcatc gtgttgtcga aggtgacgcg ccggacggcg
    11581 tcgagctttc cggcgagcga gcgcgccatc gtgaatcccg ccatcaccag aagaacacca
    11641 ccgaggaacc cctgcaaggc accgaacaga tagaccatgg ctccgtagct gtgttgcgtc
    11701 ggccgcagcc ccgtctgcca ttccgaatag acctgaacgc cgacggccgc cgcaaaaagc
    11761 aggagtgcgc tagggaagag aagctggagc gccccagagg aggggcgggc cagcgcccgc
    11821 cccgcgaaaa agatcgctgc gctgctcgcg gcatagagtg cggcggcgac ggccggccag
    11881 gcgaggctcg gcatggagac gcccggcggc ggccaggagt gggggttcac cagccacagg
    11941 aagaagtagg aaaacacgag acaggtgaaa aaggcgccct gaacgagcag aagggtgacc
    12001 gtgccccacc aggaatgatt gaccgatccc gaggtgtaga ccggcagcac gaggccgccg
    12061 ccgatgtgcg ccggccgccg cacgggtccg tgatcgagat cccacaccca ccagaggatc
    12121 agcgcgacgg ccacgacccc gaaaaacacc gccggcagga cgagcttgaa tgtcagcagg
    12181 tagaagaaga tcgtcgtcgc gatccccgcg aggaagggcc cccaatgcgg accgggaacc
    12241 tgcatgacgt agacggggcg tgcatcgatg gggctggtga cgagcgcctc gcgccggccg
    12301 gtcagcgtgc cgggaaggta atagcgcccc tcatcggtct cgtcactgag ctccggccgg
    12361 gtccacagcg gatagcggct gtcgacctcg ggaatggatc gggttccgta ggtgtaggtt
    12421 cgcatccatt ccaaagtgcc cgcatcccag atattgccca ccgtgtcgct gaacgagaac
    12481 cggaaagtgc gtacgatgtc gaaaacgagg agcgcgatgg ccgcggccat catataggcg
    12541 ccgaacgtgg agatcagatt gagcgcgccc cagtccatca ccgcaaggta agtatagacc
    12601 cgccgcggca tgcccatcag cccggtgatg tgcatcgtaa agaaggtggc gttcaggccg
    12661 atgaagaata atccgaaaac ccacctcccg acgcgctcgg aaagctgttt ttgactgacg
    12721 gtcggatacc aataataaat cgcggccagc accggcagga ccatgccgcc gaccaggacg
    12781 tagtggaaat gggcgacgac gaaataggta tcgtgcacct gcaaatcgaa tggcacggac
    12841 gcgaccatga cgcccgtcaa tccgcccatc gtgaaaacga taagaaaccc gacgatgaag
    12901 agcgcgggtg ttttcaactt cagccggccg ctggcaatcg tcgcaatcca cgcgaaaacc
    12961 tgaatgccgc ttgcaatcgc gacgctcatg ctggcggctg aaaagaagcc gagcgaaagc
    13021 tgaggcagcc ctgtcgcata catgtggtgg acccacaggc cgaagctgat cacgcccatg
    13081 gtgatcagcg ccagaacgac aaggcgatgc ccgacgagcg gcgtggcggc catcgccggc
    13141 agaatcgtgg agaccatccc cgccgcggga atgaagatga tgtagacctc tgggtggccg
    13201 aaaaaccaga acaggtgttg ccaaagcagc gggtcaccac ctttctcggc catgaagaat
    13261 ggccagccca aggcgcgctc gatctcgaga agaatggtgc cgagaatcag cgctggaaat
    13321 gcaaagacga tcatcagcgc gaaaatcagc atggcccagg caaagaccgg aattctgtcg
    13381 atcgacatgc cgggcgcccg ggttttggca aatccgacga tgatttcgat ggcacctgca
    13441 atcgcggcga tttcgatgaa gccgatcccc agcagccaca tatcggcatt gatgccaggt
    13501 gaataacggg catcggaaag cggcgtgtac atgaaccatc cggtgtcagg agcaacgccg
    13561 aagaacagcg tgaaaaagaa cgccaggctc ccgaaagcgt atatccagaa cgcgtacgcg
    13621 ctgagccttg gaaacggcaa ctcgcgcgtg ccgagtatat tcggcatcag gagaacggcg
    13681 aacgcctcca cgatcggcac ggcaaacaag aacatcatca tcgtgccgtg catcgtgaag
    13741 agctgattgt agagcgcgta atcgatgaga tcattctccg gggtggcgag ctgtgtccgc
    13801 atcaggactg cgagcacccc ggccagtatg aagatgatca gccccgtcgc tatgtaggcc
    13861 aggccgacgt atgtgttgtt gacggctgag agaatgcgcc agccgcgtgg tgggacccat
    13921 ggtttgcgaa gctcgttgag ctcgccctca gggcgcggca cctcattcgg gaaacggttt
    13981 gccggcttcc gctccgccgt ccgctcttcg ctgcggtcgc cgacgacggt catttgaggc
    14041 tctccatata ggcggcgatc gcgcgaagct gttcgccgcc gaggttgccg aatgacggca
    14101 tcagattccc gggtttcaga tgcggcgcgg aagatgtcca gcccgcgagc gttccaacgt
    14161 tgaccggata agtcccggcg gcgatcgaga gccggctgcc gacccgagtg agatcgggtc
    14221 ccaacgttcc atccgccggc gtgccgcgga tggtgtgaca cgagccgcag cccgccgcca
    14281 gaaacagatc tcgaccctgc ctgaggagcg gtgtcgtagg ctcggctgcg tttccgacct
    14341 gctcagcaaa ccagctctcg tagtcggcct gaggcacggc aacgacgtag aacgccatca
    14401 gcgcatgttg atcaccgcag tattcggcgc attgtccgcg gtagacgcca gggctcgtag
    14461 ccgcaaacgt atatctgttt gtgcgccccg ggatcatgtc gagcttgccg gcgaggctcg
    14521 gtacccagaa gctgtgaatg acatcctgcg tcttgagaat gaattcgacg ggcgtattta
    14581 ctgggatcct gatctcgttt gcggtggcga gcgcagggtt gccttggccg tcgagataat
    14641 tgacgcgcca ccagaattgc tccccgatca cctcgatgcg tatttttgga tcgctgatcc
    14701 cattcatcgt ccgtgacacg acggacgaga aggtgagcag tgccatcagc gtaatcaccg
    14761 ggaacacgat tccaaacccg atgatgaagt tgttgctcgc catccaggcc ctgaggctgg
    14821 gcctgagcag gatggccgta gcgagcagca ccgcgacgag agagaatatc agtgccccgc
    14881 ctatgaagag cacgcggagg atgccgtcga tggcctccgc gtgcgacccg cgtggggcca
    14941 gaaccgattg gctccagttg caaccggtca aaagcaggca gcctgcaccg gacgcgagag
    15001 gcgggaggag atcagggacc cgccgctgca ttcgtgctca ccataatagt gccggtgggt
    15061 aggacgtagg cagagcgccg atcgcttttg ctgtcatgaa tgaaaagacg gtcattagat
    15121 gatctgcgcg gcgagccgcg gcgcgcggtt acctttggtc ggaaacagcc gtcggctgag
    15181 ttggttccgg ccgcaccgct ggcgattgct ttggcgttgg aaccggcctc acgcgcgctt
    15241 agctgcgtga taaacgagct tgcagaaatc catctcggcg ccatcgagcg cgtgccggtg
    15301 cggcgcggcc tcgatcgcgg cctccatggc atcgggatcc gacccatcga tgaccagcac
    15361 gcgcgccggg aatatgtcga gcgccttgcg caggaacttc tcctcgctgg cgatgttggt
    15421 gacctccggc tcgacttccc aatactccac agcggtgacg gcgggtttat ccagaacatg
    15481 agccgtgaat tcttcgatcg ccggccccgg atcagccccg tcgtcgccgg cttggccgaa
    15541 gcggcaaacg acggcatagc ctccgtcagc ccagccgtgc cgcttggtca cgcgatacgc
    15601 gcctcgcaag gtgctgcgga aatgcgggag tttcctgcgg gagcgctctg ttggattgtt
    15661 gagcgctgca agataggccg ggctggcgag aacggacacg tcctcgacgt cgtagcggca
    15721 gaagtagcgc cggccgtgcc gcaagttgat atagcgccgt ccgcgaagga aaccgtcgat
    15781 cccaatccgc tcagcgtggt gctcggcatt gtaccagtcg tcgaacatgc gctccattcc
    15841 gggcgcgacg ccgtgccaga cccccaggac tccactgccg acaagtgcca tggtgtatcc
    15901 cctcgagccg ttgatagcgc taccacatat tgcaatttac cattctttca cccgctgtca
    15961 cgaaggtaag cataagcaac ttaagtttca gcctcttgaa agtggttttc aaatagtgaa
    16021 ctctcgcggc agaccaacgc tcatgcaggt ccggctcctc gggccgtgag ttcgtctctc
    16081 ggcaggccgt ggcccggccg gcagtgggcg gcacgaccgg gagcacccga tcgggcaaag
    16141 caacggggaa gaggaggctc caatgccgcg gaagaagggc gcagaactca tcacggagtt
    16201 tctggtacgg gagaaaatcc cctatgtgtt cgggatctgc ggccacggca atgtgggcct
    16261 gctcgatgag ctctacaacg tccgcaacga ggtgaagctc gtctcgccac ggcacgagca
    16321 gaccgccggg cacatggcgg acgcctattt ccgcgtcaaa caccagcccg tcgcgacact
    16381 cacctcgtgc ggtccaggct cggccaatct ggtgatggcg ctggcggtgg cgcagacgga
    16441 ctcctccgca ttcctggcga tcaccgccaa cgttccgacc tcccaattca accgcagccc
    16501 gttccaggag ctgaaccgcc attatcaggc ggacttcccg aacgtcgtgc ggccggtcgt
    16561 caagcgcgtt ttccagccga cccgcgtcga gcagcttccg ctgatgctgc ggcaggccgc
    16621 aacgacgatg accagcggcc gtccgggccc ggtcagtctc gatatcccgt tcaacgtgtt
    16681 ccaggaggag gccgacgtcg agaacgaggc gccgtggaat gggttcaacc tgcggcgcag
    16741 cggagcttct cccgaagacg tctcgacggc cgtcgacatg atgctgaagg ccgtgcggcc
    16801 tgtgctgttc gtcggccacg gcgtgacgtt gtcggaggcc ggggcggagc tgaccgagct
    16861 ggcgcaccgc ttgcaaattc cggtgatcag ctcgccgaac ggcatgggca cgatcgacat
    16921 gtcggatttg ctgtcgctcg gcttcatcgg ccgcaacggc gcctatccgg cgaaccaggc
    16981 cggccgtcac gccgacctcg tcgtggcgat cggcgcccgc ttcgacgacc gctcggcatc
    17041 atcttggcta ccgggatatt cctggaactt tccgaagacc aagctcgtgc acgtcgacgt
    17101 ggaccacgcc gagctcggcc gcaattatcc gcccgacctc ggcattctcg cagacgcgcg
    17161 gacgttcctc cggcaggtcc tcgccgaaat cgagcggcgt aacccgccgc gaaccggacg
    17221 gctcgaggca tggcatgcac agatcgcgac ctggcgcaag cagtgggacg actacgtccg
    17281 gccgaacttc gagctgcaca cctcgcccct gcggcccgag cgcgtggtgg cggatttgcg
    17341 cgcagtcctg ccggacgatg ccatcatctc actcgactcc ggcgtgcacc acaactggtt
    17401 catgcagttc tgggaggcgc gccgcccgca gacgatgctc aacacgtggg gcttctccgg
    17461 catgggcttc ggcccgagcg ggatcctggg cgccaagctg gcggcgccgg accggccctg
    17521 cgtctccgtc tgcggcgacg gcggcttcac catggtgccg cacgtcctgt gcaccgcggt
    17581 ggagtacgac attccggcgg tctgggtggt ctggaacaac ttctcctggt cggccattcg
    17641 ggacattcag tatgggctgt tctccggccg cgagatcggc accgcgttct atcagggccc
    17701 caacaacaag ccttataatc ctgacttcgc cgcctgggcg cgggcctgcg gcgtggaagg
    17761 catcaccgtc accaagtcgg aggacctcaa aggggccctc gagcacgcga tcaagctgaa
    17821 caagccggtg ctactggacg tgcatgtcga tgccgaggtc aggccgccgg ccaccggaac
    17881 ctgggcattg ccgccgatcc cgcacaagga gccggtgttc ggaaagccct acgtccccgg
    17941 cgctggcgag taacaatcga gggaggaaat gccatgcgtg ttctggtgac gggggcgagc
    18001 ggcttcatcg gctatgccgt gacggaggcg ctggcggctc gcggcgacga ggtgttggcg
    18061 acggatcagg tgatcggtcc gcggctctcc gcgctcgccg aggccaacgg caaggtgaag
    18121 gccataccgg gcgagatcac cgaatggccg catctcgtcg gcctcatcaa ggatggcaag
    18181 cccgaaaagg tcgtccattg tgcggcaatc gtcggtgtgc cggcgtccgt cggctcgccg
    18241 atcaacacgt ttcgcgtcaa tatcgaaggc acaattcatc tgctcgaagc gctgcgcctg
    18301 ttcggcgtca ggcgcatggt gaacatcagc tcggaagaga tctacggcgc ctttcaggcc
    18361 gacgtgatcg atgaagagca cccctgccgt ccgaccaagc cctacggcat ctccaaattc
    18421 gtggtcgagc agatgtcacg cgactacgcg gccgcctacg ggctcgaatg catccacacc
    18481 cgaacctgct gggtctatgg cccgtactac ccgcgccagc gggcgccacg caacttgctg
    18541 gaggcggcga tcgccggccg cccgctgcat cttccaggcg gccagtcgtt cagtgtcgac
    18601 caggtccata tcagcgatct ggtgcagggc gtgctgctgg cgctggataa gccgcagcat
    18661 cgatatgacg cctaccacat cgccactggc gtctcgacgc cgttgagcca tgtcgtggac
    18721 ctgatcaagg agcggcttcc caaggccgac atctccgtgg gccccgggcc gctcaccttc
    18781 gtggatggca cggaggtggt gcggaaaggc gcactctcga tcgcgcgcgc ccagaaggag
    18841 cttggctatt ccccgcgcta cgacatgcgc accggcatca acaactggat cgacctcttg
    18901 cttacaggca aaggatgacg cctgctttag gcgccgggca tttatggttc tcacagaggc
    18961 aaacgacgaa gtgagcctgc tggggcgcga accctgagat ggcccactag agcatttccg
    19021 aatttgacag aagcacctca cgtcaagctc gcgcccaatc tccctctctc cggcggggag
    19081 agggtcaggg tgagggggtt ccgccggtgg cagctgttgc aacccctcac ccggcccttc
    19141 gggccgacct ctccctatcg gagaggttaa cggagtgctt gctgtttgcg tttccacctg
    19201 aatcggaaaa gctctagtcc ccgtctttgc aggctcgggc gcccgcctgg gacaatgctt
    19261 cagccgggtt ttccacgacg gccgccaaga gatatgtgaa cagcgccagg ccaagggctg
    19321 cgatgtaggg cgtttccagc tcgagtgcgc cgaagaccag ccagctaaga gcgccgacgg
    19381 taatcgcggc gaatgcggcg acgggtccac cgaacctcgt gaacagcccg aacactgcga
    19441 ccacgaacac gccggcgctg cctgccgcgg aagccgtctc gaccagatcc ttcacccgct
    19501 cggctgagat ggccatggcg aacgcaaaca cggcaagacc agccagaatg gccctgttca
    19561 gcatcagccg gctgcggtcg tccagccatg gcatcaatct ctcgacgatg ttgtgtgaca
    19621 cgtgtcccga gctcgccagc agcaccgagt cgaccgtcga cagaattgcc gagatcacgg
    19681 caccggcaaa cagcacgtaa agcagccccg gcaggtgcgc ttccgcgagc aacggcacga
    19741 tctgctcggc gtcatccacg tcgggcagaa gctgcggtcc gacaagaccc aggaacaccg
    19801 ggattgcacc gacgaccaga taaatgatcc cgcccagcat ggcaccgcgg gcggcaactc
    19861 cggcggtacg tgcaccgagc acgcgcgaga taatctcgat ggccacgaca gtgccgcaga
    19921 tggggacggc gaagctctcg atgaaggtga gcaggccgtc ctctccgatc gtgagagcct
    19981 gcatccgctc cggctcgact ttcgccagac cttccgccac cccgccgatc tggccggcca
    20041 cgaccaccgc caggacgacc agcccggcca cgatcgccag accctggatg aaatcggtcc
    20101 aggcatccgc gagcaggccg cccagcacgc tgtagccgac caccagaaca gccgcgattc
    20161 cgattgccat ggacaggtcc agccccgacg cggcgctcac cacctgaccg aacgcgcgca
    20221 cctgcgccgc ggcccagaac atggagccgg gcagaagcac caggacgacc agcttttcga
    20281 cagctgatga gtaccgctcg cggaagaaat cagcgaacgt cacgatcccg cgccgccata
    20341 gcggcacggc gaaaattgca ccgaccagaa agatcgccag ggcatagccg aacggatcga
    20401 tttggcctcc cacgaggcct tcctcataga cggcgcccga ggtcccgacg atcgcctctg
    20461 caccgaagaa cgtggcaaac accgagaacg agaccagccc cagccccagg gttcggccgg
    20521 cgatcaggaa gtcggtcgtg gtcttgacgc gccttgcaac ccaaatcccg attgcgaact
    20581 ggacgaccac atacgcgatg acgccaagct gcacaccgtt catggcccgt ttccccctca
    20641 aaggccgccc ttgcgacggg ccatattccc caacgggcga tcagggtcta cgggaaattt
    20701 cggccaggtc tgccgcgctc ctctccaagg cgccggatca tcgctcgccg ccaaggttca
    20761 tccgcgataa tattgaagta gcgatgttca atactgggcg cggccatccg gcgcccacga
    20821 ccattggaga atgtgaccgc gtgcctgccg cgctcctccg ccccacgatt gcggcgctga
    20881 cggcattcgc cgtggcggcg gctcccatgt cgccgccggc gcgagcatcg gtgaagctga
    20941 cgcgggcgga ctatgaggcc tgccaagccg gcgacgagca gacgttccgg tcggccgtcg
    21001 agcgcatcac cttgaacgcc ctccagaacg gcctgaagtc gatcgacttc aaggctgttg
    21061 ttgcggccga atggcggcgc gtcggcctgg acgagatcat cgacaagcag gtcgatgtcg
    21121 ccattgccga ggtcaacgac gagtcgagct ggggcgatct catccaatcg ctggcttaca
    21181 aggaaaagtc gcaggagctg gccacagcgg tggctgagcg ggtctaccgc tcggagcctg
    21241 tgaaggcggc catcgagcag ctcgctgggg gagtcggcaa ggatgtcgga cgcaatatcg
    21301 agctggcgac gatcgatgcc gccgagccgt cattgcaatg cctgcaggca ttcttgggac
    21361 cccgtttcgg tacgactgtt tctcgcgtgg tcgccagcga tgccggcaag gaattcgcca
    21421 tcgatgcaga gacggcgcga gcgcaggtgt cgacgaccgc agtcctgatc gagggcagcg
    21481 agggcatcgc aggcgctgtc attctgttgg tgcggcgcca gctttcgagg atggcggcgc
    21541 gcatcgggca tcgcatcgtc ggcgcggttc tcggccggct ggtgtcgatc gtcgcgggcg
    21601 gggtcgggct cgtgctgatc gccaaggata tctgggagct gcgcagcggc gtgctgccaa
    21661 tcattgccga ggagatgaag tcgccggcca cgaaacagcg cgtgcgggag gagctggcga
    21721 gctcgattgc cggacagctc gatgagcagg tgcaggacat ctcggcccaa gcggccgacg
    21781 gcatcgtcga gatctggcgt gaattccgcc gcgcgcatgc aaaggtgctc gagctggcgg
    21841 agcagaacga gccgttcagg tccttcctcg acgccgcgca tcctcgtcag cttccgatca
    21901 tagacgagct ggttggcctc atcgccgcca atgagggcga ccccggcgtg ttcaagcgcc
    21961 tccaggacgg aacgctttat tccgccgtga acaatctgac cgagcccggc atgacgattg
    22021 cgcgcgagac ccgctccgtc gatgcggcgc tccgctggac cgccattgcc ggcgatcagc
    22081 tcgatcgggt taccgaatac gagctccaca agcgtgccag tgccgatgat ttcacgaagg
    22141 caacgctctc tcgtcttttt gccctcgatg accgcgttgc gacggtcagg ctcgcgagca
    22201 tcgaccgcgc gtcccgcgac gtgctgttcg acttgcagaa cgatgacctc agaagactgg
    22261 cacgcgggct tacggagccg gagctgacga ctctggcccg ctatctcact ggactccgga
    22321 agagcgcgag cgagcgggtc cttcgtgccg tggcgcaggc cccagcgaaa atgcaggtcc
    22381 tcgcatcggc acgggtgcgg gacgcggtga ttgccagccg agaccaggat gctgcggttg
    22441 ccatgatgct gcggacaggg tcattgccca atccggcact cgtcagctcg gacttcggcc
    22501 tggtcctcga tggccaggtc agtccggtgc tgctttggga gaagcactcc gttctgctca
    22561 tcgtgatgtc cgtgcttgca ttcggcggat tgctcttcct gaagcgtctt ctgttcggcc
    22621 gccgtcggag ggcagcggca tgatcggggg gaggccatct tcgtgcggct ggtgacgacg
    22681 gaaacgatcg atgagccggt gcgccacggc gagatgatct atgcctgtgc cgtgagcggc
    22741 gcgaatgtgc tccgtgacat gcgcgaggcc atcaccaaca cgatcggtgg cgagatgacg
    22801 cgttacgaag cgttgctcga caagacgatc aaaagggctt tggccaacct cgccgagcgg
    22861 gcgcgagaga agggctatga cggtgtcctc gccgtccgca tctcccatcc ggtcatcacg
    22921 aatggcgccg tcgaggtggt ggtgaccggc acgggatatt ggcgtgcagc cgattagtca
    22981 ggcaaacggc agcagcggtc actcttcacc tctcccagag ggagaggtcg gcccgcaggg
    23041 ccgggtgagg ggttgcccgg ccagctagcg tcggaacccc ctcgtcccaa ccctctcccc
    23101 gccggggaga gggagtttgg gtgcgaacgt taacccccga ggtgcgaaca tcgacccccc
    23161 ttcaatcgca ccaagtcgga gcttcagtgc agcttcaccc gtggccgggt ggtgccgcgg
    23221 agccagttgc cgatcgtcgc gaggatggcc aagctccagc cgtacagcgc cgcctggtgc
    23281 cgtttgtaga gccagcgata gatcagtctt gcgatgaagc cctccacacg caggcggggg
    23341 ccgctgacat agcccatcag agtgccgatc gcgttctcct cgccgagcga gacgagcgag
    23401 ccgaaatcgt tgtagcgaaa cggcttcagg ggctgttctg cgagaatgcg gggaatggct
    23461 tttgcgatat agcgagcctg ctgattggct gcctgcgcgc gtggcggaac ctttccattc
    23521 tttccactcc aaggtgcagc cgcacaatct cccatcgcga gcacacgtgg atctcgggtc
    23581 gtctgcagcg tctcggtgac gacgagccgg tcgaggcggt ccgtctccag cccggcgacg
    23641 tttctcaggc aatccggcgc cttcacgccg gctgcccaga cggcgaggtc cgccgggtac
    23701 cggtttccgc tcttcgtttc gatgtgggtc tgatcgaccc gcaccacctg ttcggagttc
    23761 ctcaccgaaa ttccgaggtt gcccagctcc tcggtgatgg catcggatat gcgctcggga
    23821 agctgcacca gcacacgcgg gtccagattg agaatggtca gcctgacgaa gctgtccgca
    23881 tcgagattct cgaggcggaa ttgcgtgatg acgcgaatcg acgagcgcag ctccgccgca
    23941 agctccactc ccgtcgctcc gccgccgacg atgatgacat tgaggtattc ggggcgccgc
    24001 tctggctcgg cgtagttggc ctgaaggcag gcattcacca gcgtacggtg aaattcagcc
    24061 gcctcttcga cgttgtcgag cgaataggtg tgctctttca cgccgggaat gccgaaatca
    24121 ttggtgacgc tgcccagagc cattatcgcg aaatcatagg gaaccttccg tggagggata
    24181 atcatgcgat cccggtcatg cacggcagcg acatggatca gccggttttc gcgatccagc
    24241 ccctcgaagg ccccgtgatg gaatgtgaac ccgtgccagc gcgcgaggga gaaatagtcg
    24301 atctcgtccg tgctgggata gaggctgcca gaagccacct cgtgcagcag aggcttccag
    24361 acatgagttt ggcttttatc gacgagcgcc acgtcgagca gcttgccgcg ccgcaacccg
    24421 gcgagacgga tcgcgagcag caggcctccg gctccgccgc ccacaacgac agcacggggc
    24481 cgggtgttcg gctgctccat gggcgaatga cctccgcgag aaagcaaagt aacgaggcag
    24541 ggcagcgacg gttttgccgg tggctcgggc tgttaccatg ctggcaaccc aagcaggcac
    24601 accgccgttt tccacagtcc agatggtcga aacctcaggc ggtttgatag agcttctctt
    24661 atcagaatca cggacgggcg agcaatcgca tcgtgctcag ggtcgaaaag ctgaaggtcg
    24721 gagacctgcc accgctcacc ttcgaggtgg cggcgtccga gtgcctgtcg gtcgaaggcc
    24781 cgtccggctc cggcaagaca cggctcctgc gcgccattgc ggatctcgat gacgccgagg
    24841 gttacgtttt cctggatggc gtggagcgcc gtgagttggc tgcgccggaa tggcggcgcc
    24901 gcgtgagata ttttgctgcc gagcccggat ggtgggccgc cacggcgcgc gatcactttc
    24961 cttcggatgt ggatccggca aggctcgacc gtctgctgga cgcgcttggc ctgggcgcgg
    25021 ggcttctcga tcagccgatc ggccagcttt cgaccggcga gcgtctgcga atggcactcg
    25081 tgcgcgggct catcgacgag ccgcgcgtgc tgctgctcga tgagcccacc ggggcgctcg
    25141 atgcgcaatc gagggctctc gtggacgagt tgatccgctt ccagctcctg gcgggccgca
    25201 tcgttctgct cgtcagccac aatgcggccg agatcgacag gctggcgcat cagcggctgc
    25261 agctgaaggc cctcgcgccg cggcccgccg tccctgagca gccgctgcgc gacgggagcg
    25321 ccgctcgatg acttacgttc cgctgacgtg gatcgatctg gcggcggcgg ccgtgctgat
    25381 cgtgttcaac ggcgcgctgt cctgggccct tcgcctcggg ctcgagcgca gcatcgctct
    25441 ggcatccgtg cgcatggtgg tgcaactcgg cgccgtcggc ttcgtcctca agttcatttt
    25501 cgagcagacc tcgccgcttc tgacagcagc gatggcgctc gtcatggtcg cggtcgcggg
    25561 gcacgaggtg cttgcgcgtc aggagactcg gatcggcggc tggatggcct acggcatcgg
    25621 caccggcaca ctgttcttcg tcggactggt ggcgaccatc tacagcatga tcgtcatcat
    25681 cggcccggag ccgtggtatg ccccacgtta tgtgctgccg atcctcggca tggtgctcgg
    25741 caatacactg acgggtgttt cactcgtctt gaacacgatc acccagacgg cgaagcgcga
    25801 gcgggcggcc atcgaggcgc gcctggcgct gggcgaaacg cggttcggtg cgacgtccag
    25861 cgtcgtcgtg catggcgtaa agacggggct gatgcccatc ctcaatgcta tggcggcaac
    25921 gggccttgtc acgctgccgg gcatgatgac cggccagatt ctcgtcggca tggaccctgt
    25981 cgaggccgcc aagtatcaga tcatgatcat gttcgtgatc gccgcatcga ccgcattcgg
    26041 agtaatgctg gcggccatcg ggggcgcctg gctcatcacg gacgcgcgcc atcgcctacg
    26101 cgtcgcggcg ctcggactgg tttgaggaac ccgctgcttc ggtaccgcgc cgccgatcag
    26161 ttcgcctttg ccggcatggg ccccgaccag cacaccgtcg cctccgcact gcacgtgtgc
    26221 tcatcgaaga cgatgaagtc gaggaacagc tcgcagctca cggtcttctt gctgacggtg
    26281 tacttcttga taccccgctc ggcggtccac tgtgcaatgt atttgtcgag gaggtccttg
    26341 gcatcccggg tcggaccttc cttgccgtaa tcgttgaccg aaaagcccag tttctggcag
    26401 gcggcctcaa ccggcgaaat ctgggtcagg agcagggcgg aggccaaagc agcaacaaac
    26461 gggatgcggg aacccatatc tgaaattcct cgttggcaac aggacacgaa gagccccttt
    26521 gccgacgtat atccgccggt cccatccgtt gccagcttcc tccgctttat gcaccatcac
    26581 gtgaggtcga gcgttgcaga agtgctccac ctgacgctgg tagccatttg atcgcaggcg
    26641 ctcatcggat cactccgcgt cagtgctgat acggcgcgta tccagcgttc ggggcgacgg
    26701 gagagtcgaa aaagaagccg tggtcgaacg gaccaaactc ggtttggcgg tcgaccctcc
    26761 agttccggta aaagttggtg ctgccccact tcgtgcgggg gctgcggtag gtattgatca
    26821 cgtctctgaa ggtgtaggag tacccgcctc ccggccacac gacgtagcca taagccctgg
    26881 gcttgaccac gcggtgggag gtgacttgcc cgcgcttgac atgcgagcca ttggcgtgtg
    26941 cggcgggtcc gctcatcagc catggcaccg cggcacagaa aacgacgccg aacgcgaaga
    27001 tacgcttcat cacagacctg cctttactca agggtgcata aatcaacaat acacgcgaga
    27061 cgtgaatcag cggttcactc gccaggccct atcaacgccg ccgacggtca gtggttgcgc
    27121 gcctatgagc taaattcaga ag
//
LOCUS       DASVDU010000138        18450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_397492, whole genome shotgun sequence.
ACCESSION   DASVDU010000138 DASVDU010000000
VERSION     DASVDU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397492"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="VF226_08810"
     CDS             <1..1370
                     /locus_tag="VF226_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7074126.1"
                     /translation="SEMADVVIVGAGPAGIAAADVLVSNGISPTVIDEGARPGGQAYR
                     TSVPELGISLEKLPGFEAQNHRRIHEAFARIRTSCDYRARAIAWGVFNRELYVENEGG
                     CQSIRFDKLLLAIGATDRVLPVPGWTRLGVFALGGAQVLLKDQGCLIGRRVIFCGSSP
                     LLYLAALQFKSMGGEIAAVLDTTPFRAKLAAVKDLIASPRILWRGLTYLAGLKSSGVE
                     VIHGARLHAFEGKRAVTGVTYSVGDSPPRTISCDAVAFGFGLKPETQLAQLAGCRLAF
                     DPLYRQWLPDADANGRCGDDIYVAGDGAKIGGAYAAELSGKLAAHAILQDLGMKSANR
                     PDLADVRRRLASLHRFQRGLARAFAWPADEIARLPDEVTLCRCENVTVGQLREALSAD
                     LAPREVNRAKALTRCGMGRCQGRFCGLALAELIAAGLKIPLQEVGYLRAQAPVKPLLM
                     SVAGK"
     gene            1371..2561
                     /locus_tag="VF226_08815"
     CDS             1371..2561
                     /locus_tag="VF226_08815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074127.1"
                     /translation="MRTLTTETAIVGGGLMGCWTAYFLRRRRRSVAVLEKGPVGAQAS
                     GVNFGNVRLQGRHSRQLPLAIRAHEQWEQIESLIGESCEFAATGHLYVAGDETQQTKL
                     EQYAKDAQAHGIEVELLTPAEIRSRWPWLGGRPVGGCYSARDGAANPRLVTPAVARAA
                     RRLGAEILERTKVVSVEKRASGFRLKTDCDVTIESEFLINAAGAWALDIAKMFGETAP
                     MFAAGPPQFVTEPLPYFIEPAVQLVDGSVIFRQVARGNVVVAGYPRGPSDPVANRAPV
                     PPAKTLATMRRLAETVPELAHAQVIRVWSGIEGYIPDMLPVIGPSATTEGLFHAFGFC
                     GHGFQLGPGVGLVMSELIVDGETRTPLDAFSISRFIDPLAATTWAKREEFDESLTVST
                     GGRR"
     gene            2558..3640
                     /locus_tag="VF226_08820"
     CDS             2558..3640
                     /locus_tag="VF226_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074128.1"
                     /translation="MTGTDILIFGTGAFAQRILLDIAATSAEPVNVAIAGRNEERLDW
                     LRLAANARAAIFGRPVTVTMQRVDLSSTSAASDVIGSLQPAVVVQAASAQTSSVISAQ
                     GNAWSRLVAEGGLSATAVFNALLSMRVAEAIELAKPSAHLVNCCYADVVNSLIAARGL
                     PVTCGVGNVAILWSAFSAELGLRKQGPLKVLAHYQTITPFRHPAAERKGPPPRVWIDD
                     IEIADVFARFASVKLTTAPVIEISGANGVPLMLAMARGRDCLAHVPGPNGLPGGYPVA
                     FTGGHLDLDLPSGLSREEAIRWNARFEEENGMTVDSEGHVRYTGILYDRLNAVSPVLA
                     KGFHVGDLETVFTEMEALRAQLLARG"
     gene            complement(3659..5188)
                     /locus_tag="VF226_08825"
     CDS             complement(3659..5188)
                     /locus_tag="VF226_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7074129.1"
                     /translation="MIDTSDGFVPLLQRRARSDPSGIFALYEGEPVTFAEMDNASTSL
                     AHWLRKEGVPRGGRVLVMMQNSTLAIALLFAIAKAHAVWVPVNVQSRGENLRYIVEHS
                     DPDLIIADQELVPRLEADIPGLDMERVRTAAAGRGPRSLESGLRESAEVLDWPPPAAD
                     EPYAIMYTSGTTGRPKGVIVSHRMLRFSGEGIAIVSGAGDGDVFHMWEPLYHVGGAQM
                     IVLPLIRNVRLAMVERFSASRFWGEVKQYGATHIHFLGGLLQILLKQPPGPLDRAHGA
                     RIAFGGGCPRDIWRPFEERFGVQIRECYGMTECSSVTTCNTDGVLGSVGRPLPWFEVD
                     LLDDNGSPVPWGERGEIVVRPRIPGALTVGYYKNPDATARAVRPDGFHTGDIGSFDAA
                     GYMYFHGRMTDNVRVKGENVSAFEVEHVAAQHPAVEDCAMIGVAADIGEQDIKLFVKP
                     KPGVQINPAELSVWLADRLAPYQNPRYIVVVDQFERTPSQRIMKHRLAQRSDPVWDRL
                     A"
     gene            complement(5256..7082)
                     /locus_tag="VF226_08830"
     CDS             complement(5256..7082)
                     /locus_tag="VF226_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7074130.1"
                     /translation="MTHGFAGDLQAVGAAKYRPRPAWKRLWPIYPILAFLLALFVYPV
                     VQLLSLSVFTPNGGLTADHYERLFASPVYLQVLRNTLEIAGWTTLLCIVGGYPIAYLL
                     ATTERGTRNRLILWVLLPFWTSFLVRTFAWIVLLGRRGAINGALQALGITDQPIALLY
                     NMTGVMVGMVHAMMPLAVLTMLSVMENIDRNLMPAASTLGARGGQAFWRIYFPLSLPG
                     VAAAGLLVFITSLGFFITPALLGSPRETMITQVIIQQVEEMLNYPFAGAIAMLLLATA
                     LVVFYLYDRALGMSTLAGSATTGQRGADERGRLGAGFAWIGTIMVAVFGKLSDGIAIL
                     WEKAMGSRADRPPRASGRVALRMAVLLLLIFLAGPSFFIIPVSFTTEGFIEWPPQLFS
                     LKWYGAILDHPQWIPAITRSLVVGFLTACLAMAIGAPAAFFLARQRFFAKTAALAFIL
                     SPLILPRIVIAVALFYLYARVGLIGTELGLIFGHTVLALPYVVITIMAVLKNYDQRLD
                     HAAASLGASPPRVLWYITIPLIRSGLIAAFLFAFVTSFDELTIALFVSSGLATTLPKQ
                     MWDDAILKVSPTLAAVSTLLLVFVTVVILAAEYMQRRAAARS"
     gene            complement(7079..8179)
                     /locus_tag="VF226_08835"
     CDS             complement(7079..8179)
                     /locus_tag="VF226_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074131.1"
                     /translation="MITPKLKIRELRKTYGPVVALAGANLEMPEGEFLTLLGPSGSGK
                     TTLLMMIAGLVQPDSGEVWIDGKLATYAPPHKRDIGVVFQNYALFPHLTVFENIAFPL
                     RMRGMRASEIRREVGRVLELVQLAEVGERLPRALSGGQQQRIALARCIVYRPSIVLMD
                     EPLGALDKKLRDQMKLEIKRLHDELGITVLYVTHDQEEAMVMSDRICLMNQARIEQIG
                     TPAELYFRPQTIFAADFLGESNLFDAVVEREEGAEVELSSAAGGTIRLARDPHRSFSR
                     GEAVKVMVRPETVSVLDNGKTADNIVEGRVTDAILVGGVTKYYARLADGTQISATDLT
                     RGPLAPIVRGSEIRFGWPKGGGVILPVREQGL"
     gene            complement(8279..9370)
                     /locus_tag="VF226_08840"
     CDS             complement(8279..9370)
                     /locus_tag="VF226_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074132.1"
                     /translation="MARGKRPKRFGKLDLTRRHVLAGGAAVVLVPTYWKQASAQEKRI
                     FIRDPGGPYAPGFKEAFYDPFKEATGIEAVGLQGQHEPTSLIKGMVDTQTYTWDMAHL
                     SLAACVQLRDEGYLQPLNVKSPYLDSTPAEYKNDYFAGCNVFAVVLAYRTDAFEDKGK
                     QPPQSWADFFDTEKFPGRRAMRKHPFDTIEQALLADGVPLDKLYPLDFDRAYKKLDTI
                     KSKIDVWWTGGAQTSQLLKSGEVDICPAWNARVQTAIDDGAPVKIVWNQHLWSPEGWT
                     ILKGTPKADMCREFIKFATDPARQAVFTKHLAYGPTHPDAYKWIPEDRARILPTHPDY
                     IKSRIQSDFQFWAEHKDAAAERFNAWVIS"
     gene            complement(9513..10292)
                     /locus_tag="VF226_08845"
     CDS             complement(9513..10292)
                     /locus_tag="VF226_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7074133.1"
                     /translation="MANAVLTEIADGIGTITLNKPEKLNAWDTPMRADIRRILEAWNN
                     DTGVRAVILTGAGDRAFSAGQDLDETEKFSSGEHGAQWFHSWRDFYDSFRKLEKPLIA
                     ALNGVAAGSAFQAVMLTDVRVGHPGSRMGQPEINSGIPSVLGPMLMYHRIGLSRTIEL
                     TLMGRIMEAEEAKAIGLIHHLVSTPAEVIPKAREIAEHLASKPPTALRLTKKWFREMT
                     QAAFDEAFAKGGAIQAEAYASGEPQAAMRKFFADRAKRKSE"
     gene            complement(10371..11357)
                     /locus_tag="VF226_08850"
     CDS             complement(10371..11357)
                     /locus_tag="VF226_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX7074134.1"
                     /translation="MTQNAIQIRYLSRADIERLAISGGELADRIEATIRACAAGQARN
                     FPKTSMACPDGRLFQSTMAVGVGPPAPAFAATKIVGLSPANTDRGLPHIGGVIVLNDG
                     STGMPAAIMDATWVTEMRTAALSLVAARRYARRDADSIGFIGCGAQAKSHLAVFVEAF
                     PLTRVTAYSRRRSSAEALAARARAMGLEAIVAEDPRQVVSGQAIVISTVPGHPNLVPF
                     LRADWLSRGSFGSLVDLGRSWSTEGFERIDYRLVDDKAQAEASKSYRKLTPEGPYSAD
                     LLDMVTVPTLGRKNDEERAVFTFQGVALADLAAAALVMETAARADIGQLLPM"
     gene            11476..11997
                     /locus_tag="VF226_08855"
     CDS             11476..11997
                     /locus_tag="VF226_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7074135.1"
                     /translation="MPDRLPSIETDRTPRPIHPETRIGHVHLKVADIERALDFYCGVL
                     GFELKQRYGRQAAFIAAGGYHHHIGLNTWESKGGSPPPPGATGLYHLAILYPTRRDLA
                     DALKRLIEAGVPLDGASDHGVSQALYLRDPDDNGVELYWDRPEAEWPRTPSGELAMVT
                     KKLDLDDLLSAAA"
     gene            complement(12038..13606)
                     /locus_tag="VF226_08860"
     CDS             complement(12038..13606)
                     /locus_tag="VF226_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7074136.1"
                     /translation="MSTSAPQAALDPALLTYVRQEFSGPVSAIIGYIDILEEDVPANG
                     LDHLAPDIARLRRAAVSLQQLVKQIMDSNGGLSLELSKLRHDLRTPLNAIKGYAEMLI
                     EEEPPEAFAQDLRNLLNESSRLLIRIEGLTETTGNASATASESTSADVIASSPALSMI
                     AKSAPDDAAAEIPCTCHILVVDDHESNRDLLIRRLTREGHAVTTAASGEEALSLVEQD
                     RFDLILLDLLMPGLSGFDVLTQLKNHPSHRDIPVIMISALDQIESVARCIEAGAEDYM
                     PKPFNPVLLKARINACIEKKRLREREKMFLEQLKVEKEKSEALLLNILPMAIVSRLNL
                     GETIIADHIPDSTILFADLVGFTGLSARLSATRLVKLLNMLFSEFDHLSLEFGLEKIK
                     TIGDAYMVAGGLTARNSDHTTTVAQMALRMLEVSRSTSEVLGLPLQLRIGIDTGPVVA
                     GIIGTHRFIYDVWGDTVNTASRMESHGVANEINVTEALYQRLRDTFVFEPRGTIEVAG
                     KGSMQAYFLRGARE"
     gene            complement(13635..14009)
                     /locus_tag="VF226_08865"
     CDS             complement(13635..14009)
                     /locus_tag="VF226_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7074137.1"
                     /translation="MPKILLVEDNETNRDMLSRRLIRKGFDVVMAEDGEQAVRMAASE
                     NPDLILMDMSLPVLDGWEATRRVKSAEGTRSIPIIALTAHAMAGDRERALEAGCDDYD
                     TKPIELPRLLDKIGALIARNQA"
     gene            complement(14034..17219)
                     /locus_tag="VF226_08870"
     CDS             complement(14034..17219)
                     /locus_tag="VF226_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX7074138.1"
                     /translation="MTPARLQSFEPLRLSRLSIPLRLIILSGALLTILVGTNIFMNRE
                     LKLGADALLAESRYIDNLRAASAAEKAFGDLKYWLTDAALSLLNLPEQRAREAKARLG
                     EQLAALEAHNPDTVKAIRTEVEQLFSRSLDAVEAYADDRRVIGNTLMAQSKVHIAAVE
                     KNLNQLIGTLRSEAARASEAARRRTAGAVGISWGVVIAASLLALGLTILILRSIVVPL
                     GGLTAAMKALTGGRTDIDLPPDGDDEIGQMSRTLAMFRDGLIERNRLSEEREQALKRL
                     EIARDEATAANRILQVTFDHMAQGVAMFDGDGKLLAWNRHMRDLLNLPEGLLSKGTTH
                     RTLVEFLTKRGDFGKGDAEELIRSRLEHLDQPSIQQRTLPDGRVLEIHRNPVPGRGFV
                     VMYTDVTRRVEAQREIELARNRLSDAIQSISDGFALWDAKDRLIAFNDRCAEFLKLAR
                     PLVLGMKFETLLREVAERRAEDGDRDKWVNTKLAAHRAGPSDEELQLADGTWLRVGSR
                     RTQEGGVVTTLADISALKHRELELAELVARLEVARDQADEASRTKSTFLANMSHELRT
                     PLNAIIGYSEILKEDARDQGLESFLPDIERIEAAGRHLLGIINDILDLSKIEAGRMDV
                     YIEDIDVAALVREVQSIVRPLVSKNNNKLKVICSPDIGTLRSDLTKVKQSLLNLLSNS
                     AKFTEEGTITLTIWRDETPTGATLSFEVADTGIGMKPEQMKKLFQSFTQADTTTTKRF
                     GGTGLGLAITKHFCVMLGGDITVESTPGKGSKFTITLPSRSTAEASAPADDENAQRLV
                     HGAPTILVVDDDPAALRLLSRSLVKEGYRVIEARTGKEAIDQARAHRPAVITLDVLMP
                     HMDGWSVLSALKADAELRDIPVIMITVLKERGMAFSLGASDFMTKPVDREALKSMLRR
                     YVPVGVQSPVLLVEDDPDSRETTRRVLGRLGLDVAEAENGQEGLRWLEDHPKPALILL
                     DLMMPVMNGFEFLKELQKRQEFQNIPVVVLTAKQLTAEEIEALTGSTQRILAKHGTSQ
                     VELAVAIRKCLERSGQRHGDGDKKNSSRIPA"
     gene            complement(17216..18022)
                     /locus_tag="VF226_08875"
     CDS             complement(17216..18022)
                     /locus_tag="VF226_08875"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEX7074139.1"
                     /translation="MRLRSPLRACLRPLRLERKRSLILLIAIAMLTLAPLLARSTKAD
                     EQANLDVALSLAEMLQAARTVISSNQDLINDPSRGDKGLTGDVVLAATIEEYKKIAGV
                     DPLTIDPASQHGRLLRAEMDSIKEVMDENQDLINEEGLGFKGFIPAIFGRLITERLQQ
                     KMATELRIKVTAPPPLVRNRKARPDAWEEKIIEEKFLSPSWPRGQIYYQTEDAGKEFR
                     VLVPEYYSASCLSCHGGTKGEIDITGYPKEGASEGDLGGVISITLLRPEK"
BASE COUNT         3501 a         5719 c         5632 g         3598 t
ORIGIN      
        1 gcagcgaaat ggctgacgtg gtaatagtcg gcgcaggacc tgcgggcata gctgcggccg
       61 acgtactggt ctcgaacggg atctccccca cagtcattga cgaaggtgca aggcctggcg
      121 gacaggctta tcggaccagc gtgccggagt tgggcataag cctcgaaaaa ctgcctggct
      181 tcgaggccca aaaccatcgc cgcattcacg aggcattcgc gcgcataaga acgtcctgcg
      241 actaccgtgc acgggcgatc gcgtggggcg tattcaatcg ggaactctac gttgagaacg
      301 agggcggatg ccagtcaatc cgcttcgata agctgttgct ggccatcgga gcgaccgacc
      361 gtgtgctgcc tgttcccggt tggacgcgtc tcggtgtctt tgcgctcggc ggcgcgcagg
      421 tgctgttgaa agatcagggc tgccttatcg gccgacgggt gattttctgc ggcagctctc
      481 ccctcctcta tttggctgca ttacagttca aatccatggg cggcgaaatc gccgccgtcc
      541 tcgataccac tccctttcgg gcaaagctcg cggccgtgaa ggaccttatc gccagcccgc
      601 ggatactatg gcgcggcttg acgtatttgg ccggactgaa gtcatctggc gtcgaagtga
      661 tccacggtgc gcgtctgcac gcgttcgagg gcaagcgcgc tgttacaggc gtcacctatt
      721 ctgtcggcga cagcccccca cgcacgatct cctgcgacgc ggttgccttc gggtttggcc
      781 tcaagcctga aacgcaactt gcgcagctcg cgggctgccg gctcgccttt gatccgctct
      841 atcggcagtg gctgccagat gccgacgcga atggccggtg cggagacgac atctacgtcg
      901 cgggagacgg tgcgaaaatc ggcggcgctt atgcggccga gctgagcgga aaactggccg
      961 cccacgccat tctgcaagat cttggaatga aatccgcaaa caggcccgat ctcgccgacg
     1021 tccgccgcag gctcgcgagt ttgcatcgct tccagcgcgg cctggctcgc gcctttgcct
     1081 ggccggctga cgagatcgcg cggcttcctg acgaggttac cctgtgtcgt tgcgaaaacg
     1141 taactgtggg tcagcttaga gaagcgcttt cggccgattt ggcacctcgg gaggtgaata
     1201 gggcgaaagc cctgacccgc tgcggcatgg gccgttgcca ggggcggttc tgcggtctcg
     1261 ccttggccga gctgatcgct gccgggttaa agattccgct gcaggaggtc ggctatttgc
     1321 gcgcccaggc gcccgtcaaa ccactcttaa tgtcggtagc gggaaagtag atgcggacgc
     1381 tcacgacaga aacagccatt gtcggcggcg gattgatggg ttgctggacg gcctattttc
     1441 tgcggcgtcg gcggaggtcg gtcgccgtcc tcgagaaggg cccggtcggg gcgcaggcca
     1501 gcggcgtgaa cttcggcaac gttcgcctcc aggggcgcca ttcccgacag cttccactgg
     1561 ccattcgcgc gcacgaacaa tgggagcaga tcgaaagtct cattggcgag agctgtgaat
     1621 tcgcggcaac gggacacctc tacgtcgccg gcgatgagac ccaacaaacc aagctcgaac
     1681 aatacgccaa ggacgcccaa gcccatggga tcgaggtgga actgctcact ccggccgaaa
     1741 tccgctcacg ctggccatgg cttggcggtc gtcctgttgg cggctgctac tccgcccggg
     1801 acggcgccgc gaacccgcga ctggttacac cggctgtcgc tcgtgccgcc cgcaggctcg
     1861 gggcggaaat cctggagcgg accaaggttg tatccgtcga gaaaagagcc tccggatttc
     1921 gcctcaaaac ggattgtgac gtcacgatcg agagcgaatt cctgatcaac gctgcaggtg
     1981 cctgggcgct cgacatcgcg aaaatgttcg gcgaaacggc acccatgttc gccgcgggtc
     2041 cgccgcagtt cgtgaccgag cccctgccgt acttcatcga gccggcggtg cagctcgtcg
     2101 atggcagcgt catcttccgg caggtcgcgc gcgggaatgt cgttgtcgcc ggctatccgc
     2161 gtggaccctc cgatcctgtc gcgaaccgcg cgccggttcc tccggccaag acgcttgcaa
     2221 caatgcgtcg gctggccgag accgtacccg agctggccca tgcccaagtt attcgggttt
     2281 ggtcgggcat cgagggatat attccggata tgttgcccgt catcggcccg agcgcaacga
     2341 ctgaaggcct gttccatgcc ttcggcttct gcggacacgg gtttcagcta ggaccgggtg
     2401 tcggcctcgt gatgagcgag ctcatcgttg acggagagac gcggacgcct ctcgatgcgt
     2461 tctcgatttc gcgtttcatc gacccgctcg cggctacgac ctgggccaag cgcgaggaat
     2521 tcgacgagtc gcttacggta tcgacgggag gtcgccggtg acaggcacgg acattctgat
     2581 attcggcaca ggcgctttcg cgcaacggat tctgctcgat attgctgcga cgtccgctga
     2641 gccagtgaac gttgccatcg ccggacgcaa cgaagagcgc ctcgactggc tgcggctcgc
     2701 ggccaacgcc cgcgctgcaa ttttcgggcg acccgtcacc gtcacaatgc agcgcgtcga
     2761 tctgtcgagt acatcggcag cgagcgacgt catcgggtcg ctgcagccgg cagtggttgt
     2821 tcaggcggca tcggcacaga cttcatccgt catcagcgca caaggcaacg catggagccg
     2881 cctcgttgcc gagggtgggc tgagcgcaac tgccgttttc aatgcgcttc tgtccatgcg
     2941 cgttgccgaa gccatagagc ttgcgaagcc gagcgcacat ctcgtcaact gctgttatgc
     3001 cgacgtcgtc aactcgctca ttgcagcacg cggcctgccg gtcacgtgcg gagtcggaaa
     3061 tgttgcaatt ctctggtctg ctttctccgc cgagcttggc ctcaggaagc aggggccact
     3121 caaggtgttg gcgcattacc agacgatcac gcccttccgg catccggctg ccgagcgcaa
     3181 aggtccgccg ccgcgcgtct ggatcgacga tatcgagata gcagacgtct tcgcgcggtt
     3241 cgccagcgtc aaactcacga cggcgcccgt gatcgagatc tccggagcaa atggtgtgcc
     3301 actgatgctc gcgatggcca gaggccgcga ctgcctggcc cacgtgccag gaccgaatgg
     3361 tctgccgggc ggctatcccg tagctttcac tggcggccac ctcgatctcg acttgccatc
     3421 cggtctgtca agggaggaag caatccgctg gaacgctcgg ttcgaggagg aaaatggaat
     3481 gaccgtcgat tcagagggtc acgtccgcta caccggcatc ctctacgatc ggctgaacgc
     3541 ggtcagtccc gtgctcgcga aaggattcca cgtcggcgat ctggaaactg tttttacgga
     3601 aatggaggcg cttcgggcgc aactgctggc ccgcggctga gccctgactc agttgcggct
     3661 aagctagccg gtcccacacc ggatcgctcc gctgtgccag tcggtgcttc atgatgcgct
     3721 ggctgggcgt gcgctcgaat tggtcgacaa cgacgatgta gcgggggttc tggtaggggg
     3781 ccaggcggtc ggccagccat accgacagct ctgcaggatt gatctgaacg ccgggcttgg
     3841 gcttgacgaa cagcttgatg tcctgctcgc cgatgtccgc agcgacgccg atcatggcgc
     3901 aatcctcgac agcaggatgc tgggcagcga cgtgctcgac ctcgaatgcc gagacgttct
     3961 cgcctttgac gcggacgttg tccgtcatgc gcccgtggaa atacatgtac cccgcggcat
     4021 cgaacgatcc gatatcgccg gtgtggaagc cgtcaggccg cacagcccgc gcggtcgcgt
     4081 cggggttctt gtagtagccg accgtcagcg caccgggtat acgcgggcga acgacgatct
     4141 ctccccgctc cccccaggga acgggagacc cattgtcgtc caaaagatcg acctcgaacc
     4201 acggcagtgg ccggccgacc gagccgagca ccccgtccgt attacaggtc gtcacgctcg
     4261 agcactcggt catgccgtag cattcacgga tctggacgcc gaagcgctcc tcaaaggggc
     4321 gccagatgtc ccgcgggcag ccgccaccga acgcgatgcg agcaccatgc gcgcggtcga
     4381 gcggcccagg cggctgcttc aggaggattt ggagaagccc cccgagaaag tggatgtgcg
     4441 tcgcgccata ctgcttcacc tcgccccaga atcgactggc gctgaaacgc tctaccatgg
     4501 cgaggcgcac attgcgaatg agtggcagca cgatcatctg ggcaccaccg acgtgataga
     4561 gcggctccca catgtggaag acgtcgccgt cgcccgcgcc ggacacgatg gcgatgccct
     4621 cgcccgaaaa cctcagcatg cggtgggaga cgatcacgcc tttcggccgc ccggtcgtgc
     4681 cggacgtata catgatcgca tatggctcat cggcagcagg tggcggccaa tccaaaacct
     4741 ccgcgctttc gcgcagaccc gattccagcg agcgcgggcc ccggccggct gcagccgttc
     4801 gcacacgttc catatcaagc cccggaatat cggcctcgag cctcggcacg agttcctgat
     4861 cggcaatgat aagatccggg tctgagtgct ctacgatgta gcgcagattt tcgccgcggc
     4921 tttgcacgtt caccggaacc catacggcgt gcgcctttgc gattgcgaac aacaaggcaa
     4981 tggcgagcgt gctgttctgc atcatgacga gcacgcgccc gcctctcggc acgccttcct
     5041 ttcgcagcca atgggccaac gacgtcgatg cgttgtccat ctcggcgaag gtgaccggct
     5101 cgccctcgta aagcgcgaaa ataccgctcg gatcgctgcg tgcgcgacgc tggaggagcg
     5161 gcacgaagcc atcactggtg tcaatcacgg ctatacccgg agatcacatg aagctcggga
     5221 aaatccggat gatcaggctg cttgcatacc acggttcagc tccttgccgc tgcccgcctc
     5281 tgcatgtatt ccgctgccag gatcacgacc gtcacgaaca ccagcagaag ggtcgagacg
     5341 gccgccagcg tcggcgacac cttcagaatg gcatcgtccc acatctgctt gggcaatgtc
     5401 gtggcgaggc cgctcgacac gaaaagggca atcgtcagct cgtcgaagct tgttacgaat
     5461 gcgaacagga acgcggcgat caaacctgaa cggatcagcg gaatggtgat gtaccagagc
     5521 acgcgcggcg gtgacgcacc gagacttgcg gctgcgtggt cgagccgctg atcataattc
     5581 ttcagcacgg ccattatagt aatgactacg tagggcaagg ccagcaccgt gtgtccgaat
     5641 atgagcccga gctccgttcc gatgagcccg acgcgcgcgt agagatagaa cagcgcgacc
     5701 gcgatgacga tgcgcgggag aataagaggg gaaaggatga atgcgagcgc ggcggtttta
     5761 gcaaagaacc gctgccgtgc caggaagaaa gccgcggggg caccgatggc catggcaagg
     5821 cacgccgtca agaagccaac gaccagcgac cgggtgatgg ccggaatcca ctgcggatga
     5881 tccaggatcg cgccatacca tttcagcgaa aagagctggg gcggccactc tatgaatcct
     5941 tccgtcgtaa acgaaaccgg aatgatgaag aagctcgggc cggccaagaa gatcagcagc
     6001 aacagaaccg ccatgcgcaa agcgacccgc cccgaggcgc gcggcggccg gtccgcgcgg
     6061 ctgcccatgg ctttttccca gaggatcgca atgccatcgg aaagctttcc gaatacggct
     6121 accatgatgg tgccgatcca agcgaagccg gccccgagtc gcccccgctc atcggcacct
     6181 cgctggcccg tcgtcgccga gcccgccagc gtcgacatcc cgagtgcgcg gtcgtagaga
     6241 tagaaaacaa cgagcgccgt tgcgagcagc agcatcgcga tcgcgcccgc gaacgggtag
     6301 ttcagcattt cctcgacctg ctggatgatc acctgcgtga tcatcgtctc ccgcgggctc
     6361 ccgagcaggg ccggcgtgat gaagaagccg agcgaggtga tgaatacgag caggcccgcc
     6421 gccgcaactc ctggaagtga aagcgggaag tagattcgcc agaacgcctg tccgccgcgc
     6481 gcgccgagcg tcgatgcggc cggcatcaga ttccggtcga tgttctccat gaccgaaagc
     6541 atggtcaaaa cggcgagcgg catcatcgcg tgcaccatgc cgaccatgac cccggtcatg
     6601 ttgtagagca acgcgatcgg ctgatcggta atgccgagcg cctgcagcgc accattgata
     6661 gcgccacgac gaccgagcaa tacgatccac gcgaatgtcc ggacgagaaa gctcgtccag
     6721 aacggcagca gcacccagag gatgagcctg ttgcgcgtgc ccctctcagt ggtcgccagc
     6781 aggtaggcga tcggatagcc gccgacgatg cagaggagcg tcgtccaacc agcaatctcg
     6841 agcgtgtttc gcaggacctg caggtagacg gggctggcga atagccgctc gtaatggtcc
     6901 gctgtcagcc caccgttcgg ggtgaacacg ctcagcgaga gcaactgcac gacaggatag
     6961 acgaacagcg caagaaggaa cgccaggatc gggtagatcg gccagagccg tttccaggca
     7021 ggccggggcc tgtacttcgc ggcacctacc gcctgcagat cgccggcgaa tccatgggtc
     7081 ataaaccttg ctcccgcacg ggcagaatga cccctccccc tttgggccag ccaaatcgga
     7141 tttcgctacc gcggacgatc ggcgctaacg gaccgcgcgt gaggtcggtt gctgagatct
     7201 gggttccgtc ggccaaacgc gcgtaatatt tcgtcacccc accgacgaga attgcgtcgg
     7261 ttacgcggcc ctcgacaatg ttgtcagccg ttttgccgtt gtcgaggacc gataccgttt
     7321 ccgggcgcac catcaccttg accgcttcgc cacgagagaa cgagcggtgc ggatcgcgcg
     7381 ccagccgaat ggtgccgccc gctgccgagc tcagctccac ctcggcgccc tcctcgcgtt
     7441 ccactacggc atcgaacaga tttgattcac ccaggaaatc agcagcaaag atggtttgtg
     7501 gtcggaaata cagctcggcc ggcgtcccga tctgctctat gcgtgcctgg ttcatcaggc
     7561 agatgcgatc cgacattacc atcgcctctt cctgatcatg cgtgacatag aggacagtga
     7621 tgccgagctc atcgtgcagc cgcttgatct caagtttcat ctgatcccgc agctttttgt
     7681 cgagcgcgcc aagcggctca tccatgagca cgatcgacgg ccggtagacg atgcatcgtg
     7741 caagcgcgat acgttgctgc tggccaccgg acagcgcacg tggcagtcgt tcgccgacct
     7801 cggcgagttg cacgagctcc aatacgcggc cgacctcacg gcggatctcc gaggcccgca
     7861 tgccacgcat tcgcaagggg aacgcgatgt tttcgaagac cgtgaggtgc ggaaacagcg
     7921 catagttttg gaatacgacc ccgatgtcac gcttgtgagg tggggcgtag gtggcgagct
     7981 tgccgtcgat ccagacttcg cccgagtctg gctgcaccag acccgcgatc atcatcagca
     8041 gagtcgtctt gccagaaccg gacgggccga gcagcgtgag gaactccccc tccggcattt
     8101 caaggttggc gccggcaaga gcaaccaccg gcccgtacgt ctttcgaagc tcacgaattt
     8161 tgagctttgg cgttatcact ccggatttcc ctggtcacga ccggtctgaa tcaagaggga
     8221 gcggcaggcc tcattcgacc tgccgggatg gcctgacgtt ctgcaatggg gaggcccttc
     8281 agctgatcac ccaggcattg aaccgttcgg cagccgcatc cttatgctcg gcccagaact
     8341 ggaagtcgga ctggatccgg ctcttaatgt aatcgggatg ggtcggcaga attctggctc
     8401 gatcttcggg gatccacttg taggcatcgg gatgcgttgg gccataggcc aagtgtttcg
     8461 tgaagaccgc ctgccgtgca ggatcggtcg cgaacttgat gaactcgcga cacatgtctg
     8521 ccttgggcgt gcccttcaga atagtccagc cctcgggcga ccagaggtgc tgattccaaa
     8581 cgatcttgac aggagcgccg tcgtcaatag cggtctgcac ccgtgcgttc catgctggac
     8641 agatatcgac ctcgccgctc ttgaggagct gggacgtctg tgccccgccg gtccaccaga
     8701 cgtcgatttt gctcttgatc gtatcgagct ttttgtacgc gcgatcgaaa tcgagcgggt
     8761 acagcttgtc gagcggaaca ccgtccgcga gcaatgcctg ctcgatggta tcgaaggggt
     8821 gcttgcgcat cgcgcgccgg cccgggaact tctccgtgtc gaagaagtcg gcccaggatt
     8881 gcggaggctg cttgcctttg tcctcgaacg cgtccgtcct gtacgccaga accactgcga
     8941 aaacattgca gccggcaaag tagtcgttct tgtactcagc cggagtcgag tcgagatagg
     9001 gcgacttcac gttcagcggc tggaggtacc cctcgtcacg gagctgcacg catgccgcca
     9061 ggctgaggtg cgccatgtcc caggtatagg tctgggtatc gaccatgccc ttgataaggc
     9121 tcgtcggctc gtgctggccc tgcagcccga cggcctcgat tccggtcgct tccttgaaag
     9181 gatcgtagaa agcctccttg aagcccggtg cataggggcc gccgggatcg cggatgaaaa
     9241 tgcgcttctc ttgcgccgag gcctgcttcc agtatgtcgg caccaggacg acagccgcgc
     9301 cgccggccag aacgtggcgc cgcgtaagat cgagtttgcc gaatcgtttc ggccgcttcc
     9361 ccctggccat tgttgtcctc ccttaagtga cttcgttgaa cgtggccgta aaggcattca
     9421 gagcctatgg cgaaggccct ctacagcttc cgtgactccg ccggtttcgt caagaaattc
     9481 gcgaaagcgc ttacttgcag aagcgctgtt tgtcattcgc tcttccgctt cgcgcgatcg
     9541 gcaaaaaact tgcgcatcgc cgcctgcggt tcccccgagg cataggcctc ggcctggatg
     9601 gccccaccct tggcgaaggc ctcatcgaag gccgcctgcg tcatctcacg gaaccatttt
     9661 ttggtcagcc gcagggccgt cggcggtttg ctggccagat gctcggcgat ctcgcgcgcc
     9721 ttggggatta cttcggccgg ggtgctgacg agatgatgga tgagcccgat ggccttggct
     9781 tcttccgcct ccatgatgcg gcccatcagg gtcagctcga tcgtccgcga cagcccgatc
     9841 ctgtgataca tcaacatggg cccgagcacg ctcgggatac cggagttgat ttcgggctgc
     9901 cccatgcgag acccaggatg gcccactctg acatcggtga gcatgacggc ctgaaacgcc
     9961 gagcctgcgg cgacaccgtt gagagcggcg ataagcggtt tttccaactt tcgaaagctg
    10021 tcgtagaaat cacgccagga atgaaaccac tgggcaccat gctcacctga cgagaacttt
    10081 tcggtttcgt ctaggtcctg tccggcggaa aatgcgcggt cgccggcgcc cgtcagaatg
    10141 acggcgcgca ccccagtgtc gttgttccag gcctccagaa tcctccggat gtccgcgcgc
    10201 atgggtgtat cccacgcatt cagcttttcc ggtttgttca gagtgatggt gccgatgcca
    10261 tcggcgattt cggtcaaaac cgcatttgcc atacgggcgc gccctcatgc tgcgttgcag
    10321 acatcattta caagtggggc actgatcggt gcgatctgtc aaccgcactc tcacatcggc
    10381 agcaattgcc caatgtcagc ccttgcagct gtttccatga cgagcgcggc agcagcgaga
    10441 tctgcgagcg ccacgccctg gaatgtgaaa acagcgcgct cttcgtcgtt cttccgtccc
    10501 agcgtcggaa cggtgaccat gtccagcaaa tcggcggaat acggaccctc cggggtgagc
    10561 tttcggtagc tctttgacgc ttccgcctga gccttgtcgt ccaccagccg gtaatcgatc
    10621 ctctcaaatc cttcagttga ccaggagcgg ccaagatcga cgagcgatcc gaaggagccg
    10681 cgcgagagcc agtcggccct gagaaacggc acgagattcg gatgaccagg cacggtgctg
    10741 atcacgatag cctgaccgct cacgacctgc cgtggatctt cggcaactat cgcctcaagc
    10801 cccattgcgc gtgcccgggc ggccagcgcc tcggctgacg accggcggcg gctgtaggcc
    10861 gtaacacgcg tgagcgggaa tgcctcgacg aagacggcca ggtgcgattt ggcttgtgcg
    10921 ccgcagccaa tgaacccgat gctgtcggcg tcgcggcgag catagcggcg ggccgcaacg
    10981 aggctcagcg cggccgtcct catttccgtg acccaggttg catccatgat agcagccggc
    11041 atacccgtac tgccgtcgtt gagcacaatg accccaccga tgtgcgggag gcctcgatca
    11101 gtattcgccg gcgaaaggcc aacgattttg gtcgcggcga aagccggtgc cggcggcccc
    11161 accccaacgg ccatggtgct ctggaacagc cgcccatccg ggcacgccat gctcgttttc
    11221 gggaagttcc gtgcctgacc ggccgcacag gcccggatcg tcgcctcgat cctgtccgcc
    11281 agctcccctc ctgaaattgc aagtcgctcg atgtcggcgc gagacaggta tcggatctgg
    11341 atggcatttt gggtcatgat ggggcttcgg ttcggtgcta ttcgtcaatc taccatcggg
    11401 gccgatcgtg tgcagaaaag tattgctccg ttgcagctaa cgtcgtcagt atttcaggag
    11461 atgtccatga acgagatgcc ggatcgtcta ccaagcatcg agacagaccg caccccgcgg
    11521 ccgatccacc ccgaaacccg catcggccat gtccatctca aggtggccga tatcgagcga
    11581 gccctcgatt tctattgtgg tgtgctcggg ttcgagctca agcaacgcta cggccggcag
    11641 gccgcattca tcgccgccgg tggctatcat catcatatcg gcctgaatac gtgggagagc
    11701 aaaggcggct cgccgccacc acccggcgcg acgggccttt atcacctcgc cattctgtat
    11761 ccgacccggc gcgacctcgc ggacgccttg aaacggctga tcgaggcggg cgtgccgctc
    11821 gacggcgcca gcgatcacgg tgtcagccag gcgctctatc ttcgcgatcc ggacgacaac
    11881 ggcgtcgagc tgtattggga tcggccggag gcagaatggc cgcgcacccc ctccggcgag
    11941 ctcgcgatgg tcacgaaaaa actcgacctg gacgacctgc taagcgcggc cgcgtagccg
    12001 tcagagggcg cccggattgc ctggacttgt acccgggtca ctcccgcgcg ccacgaagga
    12061 agtaagcctg catcgatccc ttgccagcga cttcaatggt gccacgtggc tcgaaaacaa
    12121 atgtgtcgcg cagccgctgg taaagcgcct ccgtgacgtt gatctcattg gcaacgccgt
    12181 gggactccat ccggctcgcc gtattcacgg tgtcgcccca gacatcatag atgaacctgt
    12241 gtgtgccgat gatgccggcg accacaggcc ccgtatcgat gccgatccgc agctgaaggg
    12301 gcaggcctaa tacctcgctg gtgctgcggg acacctcgag catcctcagc gccatttgcg
    12361 cgacggtcgt cgtatgatca gagttgcggg ccgtaagtcc accggccacc atataggcat
    12421 caccgatcgt cttgatcttc tcgaggccga actcgagcga aagatgatcg aattccgaaa
    12481 acagcatgtt cagcaatttc acgagccgcg tcgcggaaag ccgtgcggaa aggccggtga
    12541 acccgaccag atcggcgaaa agtatggtcg agtccgggat atggtcggca atgatcgtct
    12601 cgcccagatt gaggcgggat acgatcgcca ttggcagaat attgagaagc agcgcctccg
    12661 atttctcctt ttcgactttc agttgctcca ggaacatctt ttcacgctcg cggagccgct
    12721 tcttctcgat acaggcattg atgcgggcct tgaggagcac gggattgaag ggcttgggca
    12781 tgtaatcctc ggccccggct tcgatgcagc gcgccacgct ctcgatctga tcgagggccg
    12841 aaatcatgat gaccggaatg tcacgatggc tcgggtggtt cttgagctgc gtgagaacat
    12901 caaaaccact gagacccggc ataaggaggt cgagcagaat aaggtcgaac ctgtcctgtt
    12961 caactaatga gagggcctcc tctccgctgg ctgcggtcgt tactgcgtga ccctcgcggg
    13021 tcaatcgccg gatgagcaag tcccggttcg attcatggtc gtcgacaacg agaatgtggc
    13081 atgtgcacgg gatttcggcg gcggcatcat caggcgccga tttcgcaatc atgctcaatg
    13141 cagggctgga cgcgatgaca tcggcggagg tggactcgga agcggttgcg ctggcgttgc
    13201 cagtcgtttc ggtcaaccct tcgatccgga tcaaaaggcg gctggattca ttcaatagat
    13261 ttcgaagatc ctgagcgaac gcctcaggcg gctcctcctc gatcagcatt tccgcgtagc
    13321 ccttaatggc attgaggggc gtgcgcaggt cgtgcctcag cttgctcagc tccagggaaa
    13381 gccccccatt tgagtccata atctgcttga cgagttgctg aagcgaaacc gccgcccgtc
    13441 gcaatcgcgc aatgtccggc gcgaggtgat cgaggccatt ggcaggaacg tcctcctcga
    13501 gaatatcgat atagccgatg atggcggaga ccgggccgct gaactcttgc ctgacgtatg
    13561 tcagcagggc cggatcgagg gcagcttggg gggccgacgt gctcatgatg ccaactcgcc
    13621 gtgaagtatc cggctcaggc ttggttccgc gcaatcagtg ccccgatctt gtccagcaac
    13681 cgcggcagct cgatcggctt cgtatcgtaa tcgtcgcatc ccgcctccag cgccctttcc
    13741 cgatcgcccg ccatggcgtg cgcggtcaac gcgataatcg ggatcgagcg tgtcccctca
    13801 gccgacttga cgcggcgtgt cgcctcccag ccatcgagaa ccggcaggct catatccatc
    13861 aggatgaggt cagggttttc actggcagcc attcgaacgg cctgctcgcc gtcttcggcc
    13921 atcacgacat cgaagccttt gcggatcaac cggcgggaga gcatatcccg atttgtttca
    13981 ttgtcttcaa cgagaagaat cttgggcatg aacaatactc ccctgctccg ccgtcaagcc
    14041 ggtattcgtg atgagttctt tttgtccccg tccccatgtc tctggccgct cctctcaagg
    14101 cacttacgga tcgccactgc cagttcgacc tgagacgttc catgcttggc caaaatgcgc
    14161 tgcgtggacc cggtcagagc ctcgatttcc tcagcggtga gctgcttagc ggtcagcacg
    14221 acaaccggga tattttgaaa ctcctgccgc ttctgcagtt ccttaagaaa ctcaaaaccg
    14281 ttcatcacgg gcatcatgag atcgagcagg ataagcgcag gtttgggatg gtcctcgagc
    14341 cagcgcagac cctcctgccc attctcggct tccgccacat cgagcccaag ccgccccagc
    14401 acccggcggg tcgtttcgcg gctatccggg tcgtcctcga ccagcaggac aggcgattgc
    14461 acgccgaccg gcacataacg gcgcaacatc gacttgagag cctcgcgatc gaccggtttc
    14521 gtcatgaagt cggacgcccc gagcgaaaac gccattccgc gctccttcaa gaccgtgatc
    14581 atgatgaccg ggatatcgcg cagctcggcg tctgccttca gcgcgctgag cacggaccag
    14641 ccatccatgt ggggcatcag aacatcgagc gtgatgaccg ccggccgatg ggcgcgcgcc
    14701 tgatcgatgg cttccttacc cgttcgcgcc tcgatgacgc gataaccctc cttgaccagg
    14761 gatctgctca agagccggag cgccgccgga tcgtcatcga cgacgaggat ggtcggcgcg
    14821 ccatgaacga ggcgctgcgc attctcatca tcggctggcg cggaggcttc tgccgtcgag
    14881 cggctcggca gggtgatggt gaatttcgag cctttcccag gagtgctctc cacggtgatg
    14941 tcgccaccca gcatcacgca gaaatgcttc gttatcgcga gcccaaggcc cgttcccccg
    15001 aagcgcttcg tcgtcgtcgt atcggcttgc gtaaaggact ggaacagttt tttcatctgt
    15061 tccggcttca tgccgatgcc ggtgtcggcg acttcgaagc tcaaggtggc gccggtcggc
    15121 gtttcatcgc gccagatcgt gagggtgatc gttccctctt ccgtgaattt ggcactgttg
    15181 ctgaggaggt tgaggaggct ctgcttcact ttcgtcagat ctgatcgcaa ggtgccgata
    15241 tccggcgagc agatcacttt gagcttgttg ttgttcttgc tgacgagcgg tcgcacgatg
    15301 gactgcacct cgcgcaccag ggctgcaaca tcgatatcct cgatatagac atccatgcga
    15361 cctgcctcga ttttcgaaag atcgaggatg tcgttgataa ttccgaggag gtgccggccc
    15421 gccgcctcga ttctttcgat gtcggggaga aacgactcga gcccttgatc gcgcgcatcc
    15481 tctttcagga tttcgctata gccgatgatc gcattgagag gtgtgcgcag ctcatggctc
    15541 atgttggcga ggaacgtgct cttggtgcgg ctcgcctcgt ccgcctgatc acgcgcgacc
    15601 tccagtcgtg caacgagctc ggcgagctcg agctcgcgat gcttgagcgc gctgatgtcg
    15661 gccagcgtcg tcactacccc gccttcctgc gtgcgccgcg agccgacgcg gagccaggtc
    15721 ccatcggcga gctggagctc ctcgtcactg ggcccggccc ggtgcgcggc cagtttggtg
    15781 ttaacccatt tgtccctgtc gccgtcctcg gcgcgccgct cggccacctc gcgcagaagc
    15841 gtctcgaatt tcattccaag cacgagcggc cttgcgagct tgaggaactc cgcgcaacgg
    15901 tcgttgaacg cgatcaggcg gtcttttgca tcccaaagcg caaagccgtc cgaaatgctt
    15961 tgaatggcgt cgctgagccg gttgcgagcc agctcgattt ccctttgcgc ctcgacccgc
    16021 ctggtgacgt cggtatacat cacgacgaag ccacggccgg gaaccggatt gcggtgaatt
    16081 tcgagcacgc ggccgtcggg gagcgtgcgc tgctgtatgg agggctgatc gagatgctcc
    16141 aggcgggagc ggatcagctc ttcggcgtct ccttttccga aatcgccacg cttcgtcaaa
    16201 aattccacca gcgtccgatg ggtcgtgccc ttgctgagaa gaccctccgg cagattgagc
    16261 aggtcgcgca tatgtcgatt ccaggcgagc agcttgccgt cgccatcgaa catcgcgacc
    16321 ccctgtgcca tgtgatcgaa ggtgacctgc agaatgcggt tggcggccgt cgcctcgtcg
    16381 cgggcgattt cgagccgttt caacgcctgc tcgcgctctt ccgacaagcg attgcgctcg
    16441 atgagaccat cacggaacat ggccagcgtg cgcgacatct ggccgatctc gtcatcgccg
    16501 tcgggcggaa ggtcgatgtc cgtgcgcccg cccgtcagtg ccttcatcgc cgcggtgagg
    16561 ccgcccaatg gcaccacgat cgaccgcaga ataagtattg tcaggcccaa cgccagcagg
    16621 ctcgcggcga tgaccacacc ccaggatatg cccaccgctc cggccgtccg ccgccgtgcc
    16681 gcttcgctgg cgcgcgccgc ttcgctgcga agagtgccga tgagctgatt gaggttcttc
    16741 tcgaccgcgg cgatgtgcac tttggattgt gccatgagcg tattgccgat gacccgcctg
    16801 tcgtcagcgt aggcctcaac tgcatcaaga gagcgcgaaa acagttgttc gacttcggtg
    16861 cgaatggcct ttacagtgtc cggattgtgc gcctcgagag ccgcaagttg ttccccgagg
    16921 cgcgctttcg cctcgcgcgc cctttgctca ggcagattga gcaggctcaa ggctgcgtcg
    16981 gtcagccagt atttcagatc cccgaaggct ttctcggcag cactcgcggc gcgcagatta
    17041 tcgatgtagc gggactcggc gagcagcgcg tcggcaccga gcttcaactc tcggttcatg
    17101 aagatgttgg tgccgacgag gatcgtgagc agtgcgccgg acagaatgat gaggcgcagt
    17161 gggatcgaaa ggcgcgacag ccgcaaaggc tcgaagctct gcaagcgcgc cggtgtcatt
    17221 tttcaggccg caaaagagtt atgctgatga caccgccgag gtccccctcg ctcgccccct
    17281 cctttggata gccggtgatg tctatttcgc ctttggtccc gccgtgacag gacagacagg
    17341 acgccgaata gtattccggg accagaaccc ggaattcctt cccagcgtct tcggtctgat
    17401 aatagatctg tccacgaggc cacgaggggg agaggaactt ctcctcgata atcttctcct
    17461 cccacgcatc ggggcgggcc ttccggtttc tcacgagtgg cgggggagca gtgaccttta
    17521 tgcgcaattc cgtcgccatt ttctgctgca gccgttccgt tatgaggcgg ccgaagattg
    17581 ccggaatgaa ccccttgaag cccagcccct cctcattgat gaggtcctgg ttttcgtcca
    17641 tcacctcctt gatggagtcc atttcggccc gaagcagccg gccgtgctgg gaggcagggt
    17701 cgatcgtcag cgggtccact ccggcgatct tcttatattc ctcgatggtt gccgccagga
    17761 cgacgtcacc cgtcagcccc ttgtcgccgc gtgagggatc gttgatgagg tcctgattgc
    17821 ttgaaatgac cgttcgggcc gcttgcagca tttcggccag gctcaaagcg acatccaggt
    17881 tcgcctgctc gtctgccttc gtgctgcgag ccaagagcgg cgccaatgta agcatggcga
    17941 tcgcaatcag caggatgagc gatcgttttc gctcgaggcg aagcggacgt aggcaggcgc
    18001 gaagtggtga tcggagcctc atggcaggag tcctgtcggt gagtttgttg cgtcctgacg
    18061 cgtgcgccgc ggctgcccat tcgccatcgc cacctcgaac tcagacattg ggacctcacg
    18121 cgatgcgacg gcaagggtcg tccccatcca tttggcgggc actccggccc gggcaaaagc
    18181 gaattttcct ggatgccgtc cgatgcgggc aagtgcagtt tggacagcag gcccagatca
    18241 gccgcagcgc attatactcc aggtgaggag cggccgaaag ctgatgttca gcgttaatcg
    18301 atttgcgtga acgagcctgc cggcaggctg cagactggag atcaggaagg ggaattaacc
    18361 aagcggtcgc tcactcccct ggcgggctcg tgccttttgg gggggagcag caaccgagcg
    18421 cttgccgcaa aatgcaaagc agcttgcaag
//
LOCUS       DASVDU010000139        18484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_393725, whole genome shotgun sequence.
ACCESSION   DASVDU010000139 DASVDU010000000
VERSION     DASVDU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18484
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393725"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VF226_08880"
     CDS             complement(<1..877)
                     /locus_tag="VF226_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074140.1"
                     /translation="MKLSGRLLAGAVLCAFALSGPALAEPEKKDVTLGVGGKPALYYL
                     PLTVTEVKGFFKDEGLNVTIQDFQGGSKSLQALMGGSVDVVTGAYEHTIRMQARGQDL
                     VAVIELGRFPGIVVGVRKELADKVNSAADLKGMKIGVTAPGSSTHNIVNFALQKAGLS
                     PRDVSIVGVGASSTAVAAMEKGEIDAISNIDPAISMIDKKVGLKVIVDTRTEEDTIKL
                     LGAPSPAAVLYLRPEFAEKNPKTVQALVNAFYKGLQWIANASAEEIAAIMPESYYLGD
                     REMYVKAVASSLPTYS"
     gene            complement(901..1692)
                     /locus_tag="VF226_08885"
     CDS             complement(901..1692)
                     /locus_tag="VF226_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074141.1"
                     /translation="MNRNKILFLRVLTAIGMLVAWHVLTTVPAPNEPLLDPFFFSTPV
                     DVLERTAGDIVTYQMWYDLYITLLETALAFFIGVVSGIALGFWFARRELLAAVMDPFV
                     QAANALPRVVLAPIFALWFGLGIWSKVALGVTLVFFIVFFNVYQGVREVSPTVLANAR
                     MLGMNDRHLLRHVYLPSAMSWVFSSLHTSVGFALIGAVVGEYLGSAAGLGYRIAQAEG
                     VFDVTGVFSGMLVLMIFVVLIDGLVTVCERRLLVWRPTAAATRTT"
     gene            complement(1689..2531)
                     /locus_tag="VF226_08890"
     CDS             complement(1689..2531)
                     /locus_tag="VF226_08890"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074142.1"
                     /translation="MSDPAVAEERQHNSASLAVSLRDIRITFADRSGQSYTAIADADL
                     DVRDGEFVAIVGPTGCGKSTLLNVAAGLLNPSAGRCAIFGSQLKGLNRKAGYLFQADA
                     LMPWKTALENVAIALEVNGTPAAEARERAVEWLGRVGLSAFTGRYPHQLSGGQRKRVA
                     LAQVLILDPKILLMDEPFGPLDAQTRTLMGELLLDLWSRDRKAVMFVTHDLEEAISLA
                     DRVVVMAAGPESRVIGDFNVGLERPRDPAEVRHTAEFATIHKEIWQILKKEVIKAYAT
                     EDEA"
     gene            complement(2736..3353)
                     /gene="leuD"
                     /locus_tag="VF226_08895"
     CDS             complement(2736..3353)
                     /gene="leuD"
                     /locus_tag="VF226_08895"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7074143.1"
                     /translation="MEAFTVLKSRGVAVLLDDIDTDMLFPAQYCKTLETTGLGKYLFA
                     RERFPEYNPDYVKFPLNRPEHAGAQVLVSGRNFGCGSSREHAVWALRDYGFRCIVAES
                     FSDILYANCFKNGMLPVVLPRETVDDLARLTQAAPDAVFTIDLYEQTVTAGDLGPFRF
                     SIEPHRRHMLLKGLDEVGLTLAQAQAIETFEKSHFASQPWLLRAG"
     gene            complement(3364..4767)
                     /gene="leuC"
                     /locus_tag="VF226_08900"
     CDS             complement(3364..4767)
                     /gene="leuC"
                     /locus_tag="VF226_08900"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7074144.1"
                     /translation="MPRTLYEKIWDSHVVEERPDGSALLYIDRHLAYEITSPQAFAAL
                     RKAGRRVRRPELTLAVADHGVPTIGSRSRPLADAGSRMQVETLQANCAAFGITYIPID
                     DQRQGIVHVIGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLV
                     QVRSKSLRVEVDGELPRGVAAKDLALAIMAKVGPHGATGHVIEYCGSTIKNLSMEGRM
                     TVCNMSIEAGARAGLIAPDETTFAWLKGRPMAPRGSDWDAAVRYWSTLKSDDGAHFDR
                     SVAIDASSLAPWVTWGTRPSQGGAVTGRVPRPEDAENEAEAAAISRALSYMDLQPGTR
                     LTDIKVDRIFIGSCTNSRLEDLRAAAAVARKGRVADGVRAIVVPGSGLVRRQAEEEGL
                     DRVFQEAGFEWRLPGCSMCVAVNGDQVEPGKRCASTSNRNFEGRQGPGARTHLMSPAM
                     AAAAALAGRIVDVRDYL"
     gene            5037..5327
                     /locus_tag="VF226_08905"
     CDS             5037..5327
                     /locus_tag="VF226_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074145.1"
                     /translation="MIRLRTIAITATVLACLSSAGDGAQAQSAAAAAAADPEFQSLLQ
                     RMYTDSQDMETTFRFLEVATRLGDYEAAIGALERVLYYNPDLAEVKVELARP"
     gene            6341..7159
                     /locus_tag="VF226_08910"
     CDS             6341..7159
                     /locus_tag="VF226_08910"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7074146.1"
                     /translation="MKAKRTSSRLKLVAQSAETDIPKHRAIADLLLQEIEAGRWGPGE
                     RIPSEEQLVAETGASLGTIQRALRNLVELGIVVRRHGLGTFVVGGRAPFRHLRHFRFL
                     AEDGVRLLPVFVKVIDVTIIQDTGPWSDFLGEPHEGYVRIKRKVDVGGEFDLYSEVYL
                     PADRFGALAELSFEELDGCSIRDCLAERFNAPTLKTHQTMLCQPLPLRAARVIGVPHG
                     TYGFVWVICAASYRDAPITWQRVFIPPNDNQLELLSGNDLPSPGRQSSNRLRKE"
     gene            7178..7984
                     /locus_tag="VF226_08915"
     CDS             7178..7984
                     /locus_tag="VF226_08915"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEX7074147.1"
                     /translation="MSREPPFAMITLPPERSAAKPRKTGLTMMADWGLPISYQEDLLA
                     LGGRYIDFAKIVTGTARLYERDYLEKKLALYQLHQVRSFIGGQFFEYVLATQGWGALD
                     AFFDEAVAIGFQTIEISDNCIPLGDDERVSAVELARKHGLAVMGEVGSKNDDSDIRLL
                     IAQARQLFEAGAEFVLVEAAELVEAGKPRADKLDALREALDLTKVMIELPGPWISGIT
                     LSIVQDLKKALIASFGPDVNLANIHAEDLIATEALRVGLGVVGPTTRLAG"
     gene            8103..9059
                     /locus_tag="VF226_08920"
     CDS             8103..9059
                     /locus_tag="VF226_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7074148.1"
                     /translation="MWKRPIIQSICAAILAIGTGVAASAQDYPSKPIKLVVGFGAGGM
                     TDTSSRMIAEELDKKLGTRIVVQNQPGVGGLLALDAVKREPADGYTIVTFLSDSPFTA
                     AYQGKSLNFDDWTMLGGYMPQERVLFGRQDNPFHSLDELISYAKKNNVTFADGGSFWS
                     ARVMEAFAKQNGLKMRLVPFKSGAEGSAAILGGHVMLAETGVGTPAWTSSKDKGLKIL
                     ATLTPGGLADFGLPDVPTLKSAGANFVVDFYYGLAINSNTPPERVKVLRAALQEVIED
                     PAVQERFRKIDLVLKWMPGDAYLERMKEVAKSAAGLKDYLQK"
     gene            9314..11554
                     /locus_tag="VF226_08925"
     CDS             9314..11554
                     /locus_tag="VF226_08925"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEX7074149.1"
                     /translation="MPFSHYLYDRLGRAEISPKGPHVAGSHVPLVLTYTAGVVGIDDS
                     GALKFSWRTASDSGKPQFTDPRAPNYTTAYATNGAKLHIEYNRNNIRPWVNTLFVRVV
                     AGFLREGDQIIVKLGDPSHGSPGYRLQTCCDDDFQFKTFVDAFATYDFVPIPLSPAIK
                     LISGKPARWKAYLPTLRRTRDAFRLVIVPEDRWGNPSDQADQTILLSSNMPVEGLPDR
                     VVFARGDGPRVIDGLRVDKAGVLEISLMGADGSPLGSSNPLRLAEDVPMLHYWGDLHG
                     QSNETVGTNTVEQYFAFARDRAFLDIVAHQGNDFQIDDELWAKINDLSAQYDEPGRFV
                     ALPAYEWSGNTGMGGDHNVYYRAEGRPIYRSSKVLISSETKDPVCLNVNELFAALEKE
                     DAVVIAHVGGRYADISIGHSGRIQQSVEIHSCWGTFEWLLHDAFDLGYRVGLVCHSDD
                     HKGRPGAAYPGASTFGAIGGLTCYIMPELNRDNLFEALRARRHYGTTGTRLYLDVKAQ
                     FDGIAERFETDPALGPTQSKSVRAATMGDIVRTTDSSVKLAVEVIGSAPIERVTIFNG
                     KSPIAVHRNFDSSELGHRIRVLWEGAEYRGRAREVYWRGHCRLEGNRFTRTESFNLFN
                     VDKPLRVAEDGTRVDFDSVTTGNFAGFDLWLDDREAGELIFASDVLDARVPIAEIGME
                     DKVVDAGGLGKRLRIFRLPDVNSSRQLSFTHHIELRRDRDNPIYVRVTQEDGHQAWSS
                     PIYLIP"
     gene            11612..12073
                     /locus_tag="VF226_08930"
     CDS             11612..12073
                     /locus_tag="VF226_08930"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7074150.1"
                     /translation="MKDQKSLFAGGIFVAVGCIYGLTAWWSLPVGDSVNMGPGYFPIV
                     LSMLLVLLGAAIAVRSVVGEYSVAFEGIPWRSIAMNTLAILCFASLIRPLGLFPTVML
                     TASLSCLGSANVSMTKAAIAGFSIAVLCTLIFSFIIGLPIAIVGPWLGGFI"
     gene            12077..13582
                     /locus_tag="VF226_08935"
     CDS             12077..13582
                     /locus_tag="VF226_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017263942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7074151.1"
                     /translation="MELLTHLAIGFATAFSPSNLLYCFLGVLLGTVIGVLPGIGTATT
                     VAMLLPLTFQLEPTSSLIMLAGIYYGSQYGGSTTAIMVNLPGEASSAVTTIDGYQMAR
                     RGRAGPALAVAALGSFFAGTVATLLVLTVAQPLTAIALRFGPAEYFSLVTVGLVSSVA
                     LASGSILNALAMICLGLLISMTGTDIYTAMPRLTFGLPELEGGIDVAAIAAGAFGIGE
                     IIRNSEAGVIARSTIASVKSIWPSIEDLKRMVGPTVRGTTIGSFLGVLPGSGALLSSF
                     VAYNVEKKVSRNSAEFGKGAIEGVAAPEAANNAGAQTSFIPMLSLGLPSNVVMALMIG
                     AMMMQGITPGPNVATTHPELFWGVIVSMWIGNAMLVLLNFPLIGIWIKLLSTPSWILY
                     PAIVTFCCIGIYSLANNSFIMYILLVSGVGGYILVKLGCELPPFILGFILGPMLEEHF
                     RRAMSLSGGDPMILVTRPISAALLAVAVVALIVVSLPAISRKRKEVFVEED"
     gene            13833..14630
                     /locus_tag="VF226_08940"
     CDS             13833..14630
                     /locus_tag="VF226_08940"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX7074152.1"
                     /translation="MTRASTSEDDVISVYWQTGCTSCLRTKEFLTRHGVAFRSRNVLE
                     DQEALDELARFGLRRVPIVVRGDKWADGQVLRDVAKLCGIAWGNEKILPPDELCRRVN
                     AIIKATQANVLHLPEDSMQTQLPNRPRSYADLVYHIFNIVDAFVEHEEGIPLAYEAYY
                     RVPPEEMRTPKGLAEYGEKVGERFNGWFAEHGETADWARKADVYYGQQSLHEFLERTT
                     WHSGQHARQLVWVLERMGIAPAQPLHSEVFRGLPMPEKVWDDEPVTS"
     gene            15659..16930
                     /locus_tag="VF226_08945"
     CDS             15659..16930
                     /locus_tag="VF226_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX7074153.1"
                     /translation="MVESRRTAPLETILEHLSLRPATGCEDLISFKSSGQLTLGVEIE
                     LQLVDRTTLNLAPRAEEVLACAGDLPNLTKEFYLSTVEINSRKCADVHEISSDLHAIL
                     ERVDEIGDRLGISFSTTGCHPFARYADCIITPTPRYNELIDRNQWLTRRMTVYGMHVH
                     LGMSSGDECIRFNNFFLHFLPHLLALSASSPFWQGEDTGLASCRPTTYEALPTAGQPY
                     VVHSWREFGQLCSTLRACDAIQSLKDLWWDLRPSPAHGTLEIRICDGLASLSETLAVV
                     AFVHALAHWFGDHATWLEKVPPPPRWLARENKWRAVRFGLDAKLITSLDGTVKPLRDD
                     VLDWCERIAGNVAALGYEPYFATLRQILADGNSSMRQRAVYARTGSLEQVVQQNIAEF
                     RARAPLWDKSPLESPVRSQLEFSLDRTHDGP"
     gene            17205..>18484
                     /locus_tag="VF226_08950"
     CDS             17205..>18484
                     /locus_tag="VF226_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074154.1"
                     /translation="MDFVAGADSVLAAVGALGADSWQNAAAFAAVLAVILYALRANGF
                     LARVWRAVQDTILTNWQLALLGAAAIVLSLASGWRTWDGMRNFTGESALSLMITFGIQ
                     AVMLIVAWLIGESFATGMSQQVDGAKRIRKPMDGAIGMVLGLAISGAAFYWLLHVFGA
                     VSWTRTADLAFDWSRFATIAMYFAIALLVVGALAFSFSRGGELAVPYVRGTRVIARNV
                     VLWVMLLACMGTSVFFSFDSFFSSIFPEDQRRRSAELRAQNQVSGILADTGATIASRR
                     VTEAARLFETAEWKAYDAQLTELAQLAQSSSSALKAYFLQELEQRRRLVAEQLERKAT
                     AQSGQAGLLTRKTALIEELARARSERPGLAAEYAQHKSELDAKAREVDAKRVEAMAEA
                     RGVEGTGKEGRGPVYRQRMAELDRLQGEYDIKLER"
BASE COUNT         3612 a         5519 c         5583 g         3770 t
ORIGIN      
        1 gcgaatacgt cggcagggag ctggccacgg ccttcacgta catctcgcgg tcgccgagat
       61 aatagctttc gggcatgatg gccgcgattt cctccgcaga cgcgttggca atccactgca
      121 ggcccttata gaaggcattg acgagcgcct gtacggtttt ggggttcttc tcggcgaatt
      181 ccggacgaag atagagcact gcggccggag agggggcgcc gaggagcttg attgtatcct
      241 cctccgtgcg cgtatcgacg atcaccttga ggccgacctt cttgtcaatc atcgaaatgg
      301 ctggatcgat gttcgaaatc gcatcgatct cgcccttctc catggccgca acggccgtcg
      361 agcttgcacc aacaccgacg atggagacgt cgcgcgggga gaggcccgcc ttctggagcg
      421 cgaagttcac gatattgtgg gtcgaggagc cgggcgcggt cacgccgatc ttcatgcctt
      481 tgaggtctgc cgccgaattg accttgtcgg cgagctcctt ccgcacaccc accacgatgc
      541 cagggaaccg acccagctcg atcacggcca caagatcctg cccgcgggcc tgcatgcgga
      601 tcgtgtgctc gtaagcgccc gtcaccacat cgacggatcc gcccatcaaa gcctgcaagc
      661 tcttggagcc accctggaaa tcctggatgg tcacgttgag cccctcgtcc ttgaagaagc
      721 ccttcacctc ggtgaccgta aggggcaggt aataaagcgc aggcttaccg ccgacgccga
      781 gcgtgacgtc cttcttttcc ggttccgcca acgcaggacc ggacagtgca aaagcgcaga
      841 gcaccgcgcc cgctagcagt cttccagaga gtttcatcct attcctcctt gagcttcagc
      901 ttaggtcgtt ctcgtagccg ctgctgttgg acgccagacg agcaggcgcc gttcgcatac
      961 ggtgacgagt ccatcgatca ggaccacgaa aatcatcagc accagcatcc cgctgaagac
     1021 gccggtgacg tcgaacacgc cctctgcctg cgcgatgcga tagccgaggc ccgcggcaga
     1081 gccgagatac tcgccgacga ccgcgccgat cagcgcaaac ccgaccgacg tgtgaagtga
     1141 ggagaaaacc caggacatcg cggagggcag atagacatgg cgaagaagat gccggtcgtt
     1201 catcccgagc atccgagcgt tggcgagaac ggtggggctc acctccctga cgccctgata
     1261 gacgttgaag aagacaatga agaaaacaag tgtcacgccg agagcgacct tggaccaaat
     1321 gcccagacca aaccataggg cgaagatcgg tgccagcacc acgcgcggaa gcgcgttggc
     1381 cgcctgcacg aagggatcca tcaccgccgc caacagctcc cgccgcgcga accagaagcc
     1441 cagcgcgatg ccgctgacga cgccgatgaa gaaagcaagc gccgtctcca gcagagtgat
     1501 gtagaggtcg taccacatct ggtatgtgac gatgtcgccc gctgttcgct ccaggacgtc
     1561 aacgggcgtc gagaaaaaga agggatcgag gaggggttcg ttcggcgctg gtacggtcgt
     1621 caagacatgc caggcgacaa gcattccgat cgcggtaagg acgcgcaaga agaggatctt
     1681 gttccggttc atgcctcatc ctctgtcgcg tatgccttga tcacttcctt cttcaagatt
     1741 tgccaaatct ccttgtggat cgtcgcgaac tcagcggtat gtcggacctc ggcggggtcg
     1801 cgcggccgct ccaggcccac gttgaaatcg ccgatgacgc gggattccgg tccagccgcc
     1861 atcaccacga cgcgatccgc gagcgatatc gcctcctcga gatcgtgcgt gacgaacatt
     1921 acggccttgc gatcgcgtga ccatagatcg agcagcaatt cgcccatcaa ggtgcgggtc
     1981 tgcgcatcga gaggcccgaa gggctcgtcc atcagcagga tcttgggatc gaggatgagc
     2041 acctgggcga gtgccacgcg tttgcgctga ccgccggaga gctgatgcgg gtaacggccg
     2101 gtgaaggcgc tcagccccac gcgaccgagc cattccaccg cgcgctcgcg ggcttccgcc
     2161 gctggtgtcc cgttcacttc cagcgcgatg gcgacgttct cgagggcggt cttccacggc
     2221 atcagcgcgt cggcctggaa gagataaccc gccttccggt tgaggccctt gagctggctg
     2281 ccaaatatgg cgcaccggcc cgccgagggg ttcagcagtc ccgcagcgac attgagcaac
     2341 gtggacttgc cgcatcccgt cggcccgacg atggcgacga actcaccgtc ccttacgtca
     2401 aggtctgcat ccgcgatcgc ggtgtagctt tggccagatc gatccgcaaa ggtgatgcgg
     2461 atatcgcgca gcgacaccgc caggcttgca gaattgtgtt gccgctcctc agccacagcc
     2521 ggatcgctca tatgtccgac taaggcctgc tctcccatca atctccccct tccacgcgaa
     2581 gcaccatcga cgcgttctta gcatgacggc tcgtctggtc accattactc gattggccac
     2641 gtcttactat cagtgcttgc ccatcaagca tttaacggac tggcagacaa gcggggggcc
     2701 tcgaggccga tcaacgattg cgagctgaag cggcttcagc cagcgcgaag gagccagggt
     2761 tggctggcaa aatggctctt ctcgaaggtt tcgatggcct gagcctgtgc cagcgtgagc
     2821 ccgacctcat cgagcccctt cagcagcatg tgccggcggt gcggttcaat cgagaatcga
     2881 aatggcccaa ggtccccggc cgttaccgtc tgctcgtaaa gatcgatcgt gaacacggca
     2941 tcgggagcag cctgggtaag cctcgccagg tcgtctacgg tctcgcgcgg caggacaacc
     3001 ggcagcatgc cgttcttgaa acagttcgca taaagaatgt cagagaagct ctccgccacg
     3061 atgcagcgga acccgtagtc gcgcaacgcc cataccgcat gctcgcgaga ggagccgcag
     3121 ccaaaattcc tgccgctcac cagcacttgc gcgccggcat gctcgggtcg gttgagaggg
     3181 aatttgacat agtcggggtt gtattctgga aaccgctcgc gcgcgaagag gtacttacca
     3241 agaccggtcg tctcgagcgt cttgcagtac tgggccggga aaagcatgtc ggtatcgatg
     3301 tcgtcgagca gcacggcgac accacgcgat ttcaggaccg tgaacgcttc catgccgttt
     3361 cactcagagg taatcgcgaa cgtcgacaat ccggcctgcg agtgccgcgg cggcggccat
     3421 agccgggctc atgagatgcg tgcgcgcacc cggtccctgg cggccttcga aattgcggtt
     3481 cgaggttgaa gcgcatcgct tcccgggctc aacctgatct ccgttgacgg cgacgcacat
     3541 ggagcagcct gggaggcgcc attcgaagcc cgcttcctga aagacgcgat cgagcccctc
     3601 ttcctcggct tgccggcgca ccagtcccga acccgggacg acgatggccc gcacgccatc
     3661 tgcgacgcgc ccctttctcg ctacggcagc cgccgcgcgc aagtcctcca accgcgagtt
     3721 cgtgcaggag ccaatgaata tgcggtcgac ttttatgtcc gtgagccgcg ttcctggctg
     3781 aagatccata tacgagagcg cacgcgagat ggcggctgcc tcggcctcgt tttcagcatc
     3841 ctccggccgc ggcaccctgc cggtcaccgc tccgccctgc gaaggtcgtg ttccccaagt
     3901 gacccaagga gccagactgg acgcatcaat cgccaccgag cggtcgaaat gcgcaccatc
     3961 atcgcttttg agcgtcgacc aatagcgcac agcggcatcc caatcgctgc cccttggcgc
     4021 catgggccgc cctttgagcc atgcgaacgt ggtttcgtcg ggcgcaatca ggccggcacg
     4081 cgcacctgcc tcgatggaca tgttacagac cgtcatccgg ccttccatcg agaggttttt
     4141 gatggtggac ccgcagtatt cgatgacgtg acccgttgcc ccgtgtgggc ccaccttcgc
     4201 catgatggcg agcgccagat ccttggcggc gacgccgcgt ggcagctcgc catcaacctc
     4261 gacccgcagg cttttcgagc ggacctggac gagggtctgc gtggcgagga cgtgctcgac
     4321 ctcgctggtc ccgataccga acgcgagggc cccgaaagcg ccgtgggtgg aggtgtgact
     4381 gtctccacag acgatcgttg cgccaggcaa ggtgaagccc tgctccggcc cgatgacatg
     4441 aacaatgccc tgccgctggt cgtcgatagg gatgtaggtg atgccgaatg cggcacagtt
     4501 ggcctgcaga gtctcaacct gcatccggct cccggcatcg gccagcggcc gactgcggga
     4561 tccgatggtc ggcacgccat ggtcggccac cgcgagcgtc aactcgggtc gccggactct
     4621 gcgtcctgcc ttcctcaggg cggcgaaagc ctgcggactg gtgatttcgt acgccaagtg
     4681 tcggtcgatg tagagaagcg ccgatccgtc cggccgctcc tcaaccacgt gactgtccca
     4741 gattttctca tagagcgtgc ggggcacgtt cctacctcgg tgtaggtgat acctgtaacc
     4801 gccagccgcg ctttatcacg ccgagcagag ttgcggaagg gaatcgccgc tcgaagccgt
     4861 gccgatgtcg atggagttgt cgaatgtcgc gccctccagg tcggcttctt ctccgagctg
     4921 ggtcgctcca ccgagactca agaccatcgg tgttgtggga tgatcggtca ttcaaccgag
     4981 agttactagt caaaccctga agacttaatc cagtcgaggg gattcggggg ccgcccatga
     5041 taagactacg taccattgcg ataaccgcga cggtcctggc gtgcctgtcc tctgcaggag
     5101 acggtgcaca ggcgcaatcg gctgccgcag ctgccgcggc cgaccccgaa tttcaatcct
     5161 tgctccagcg catgtacacc gactcgcagg acatggagac aaccttcagg ttcctcgagg
     5221 tcgccacgcg gctcggggac tacgaagcgg ccatcggggc gctcgagcgg gtactgtact
     5281 acaacccgga tcttgctgag gtgaaagtcg agctcgcgcg gccttaatct ccgcattggc
     5341 ggcaatcagg tcgcgcagag ctacctcgag caggccctga caacgccagg cgcggttccg
     5401 gaaacggttg ctgccgcccg ccagcttctg acgcaggcag acactgatac agcggatgca
     5461 acagcggatg ccctgcgcgt cttcatcttc gccggtacgc gctatcagac gaacgcaccg
     5521 cgggaccgaa aagcgatctc gtcctgtcgg aaaacggcga tctgctaccg atcgagcctg
     5581 gctttgcgcg tgcggatgac cggaacgcgg ttgcactccc ttccatgacc tattttcggc
     5641 cgaaatcggc gtgttcgggt attacgccaa gcaggtcgac ttttctgaat tcgtcgtcaa
     5701 ggatctgtca cagacgctgc aaacgacgat cggcactacg gccgtgccgt ccttcccggc
     5761 ttactgcaac ggcccacaat aaggcttttg cgtcggcttt gttccggaaa ccatacccgc
     5821 catcttgctg gtttgggaat ttggtggcgg gttcggtcag ctcgttatct tgcgccgctg
     5881 acgggggacg acatccaggc ggtgccaatg gcaccgggat gacgctggcc aatccaaagc
     5941 tgcaggccga gtcatgccag catgccggcc tcagccatgt gctttgtcac gtcgaagcgt
     6001 gctgctgtac gctataggtc tgatcggcgc ggtcagtcat tcgtctgcac ttctgctgaa
     6061 gcaccttgag cattgcgtcg atcagctcgc tgtcacctca tccgaaatgg atgccgatcg
     6121 cgaacgcctg ggcgctcgct acaaatcctg caccgaaaag atcgaattat gattgaaccg
     6181 agacagaatt ttccaacacc ccacttcacc ctccggcggt gggcagttgg ggtcaggctc
     6241 accccctggc ctcagtgagg ccgtctttca gtcctctata ggtgttgaca atttgtcgat
     6301 tggggaatag cgaatgtaag atccatcgaa gcaaatggtc atgaaggcga aacggacttc
     6361 atcccggttg aagctggtcg ctcaatcagc ggaaaccgat atccctaagc atcgggccat
     6421 cgccgacctc ctgctgcagg agatcgaagc cggtcggtgg gggccgggcg agcgcattcc
     6481 ttcggaggag cagctcgtcg cggagaccgg cgctagtctg ggaacaattc agcgagccct
     6541 caggaatctc gttgaactcg ggatcgtggt gcgtcgccac gggctgggta ccttcgtggt
     6601 cggcgggcgc gcaccatttc gccatctgcg tcatttccgt tttctggccg aggacggagt
     6661 gcggctgctc ccggtattcg tcaaggtaat cgatgtaact attatccagg ataccggtcc
     6721 ttggtccgat tttctcggcg aaccgcacga aggctacgtg cgcatcaagc gtaaggtcga
     6781 tgttggcggc gagtttgacc tttatagcga ggtctacctc ccggccgatc gatttggagc
     6841 gctggccgag ctatcatttg aggagctgga cggttgttcg atccgcgatt gcctcgccga
     6901 gcgatttaac gctcccacgt taaaaaccca tcagacaatg ctttgtcagc cgctgccgct
     6961 ccgagcggcg cgggtcatcg gtgtgcccca tggcacctat ggttttgtct gggtgatatg
     7021 cgcggccagc tatcgcgacg cgccaatcac ctggcagcgc gtcttcattc cgccgaacga
     7081 caatcagctg gaattgctca gcggcaacga tctgccgtct ccggggaggc agtctagcaa
     7141 ccggttacgt aaggaataaa cttctgagga gacgctcatg agtcgggagc cgcccttcgc
     7201 aatgatcacc ctccctccgg agcgcagcgc cgcgaagcct cgcaagaccg gcctaaccat
     7261 gatggcggat tggggactgc caatctccta tcaggaggac cttctcgcgc tgggcggccg
     7321 ctatatcgat tttgcgaaga tagtaacggg gaccgcacgc ctttatgagc gcgattatct
     7381 ggagaagaag ttggcgctct accagttgca tcaggtccgg tcctttatag gaggccagtt
     7441 cttcgagtat gtccttgcca cccaaggctg gggtgccctg gacgccttct tcgatgaagc
     7501 ggtggcaatc ggattccaaa cgattgaaat ttctgacaac tgcataccgc tcggggacga
     7561 cgagcgtgtt tccgcagtcg aattggcgag gaagcacggt ctggcggtca tgggcgaggt
     7621 tgggtcgaag aacgacgata gtgacattcg gctcttgatc gcgcaggcgc gccagctttt
     7681 cgaagccggc gccgagtttg tgcttgtgga agcggctgaa ctggtcgagg cgggcaagcc
     7741 aagggctgat aagctcgacg ccctgcgcga agcgctggat ctcaccaaag tcatgattga
     7801 gctgccaggt ccctggataa gcgggattac gttgtccatt gttcaggacc tcaagaaggc
     7861 gctgattgcc agcttcggcc cagacgtcaa tttagcaaac atccatgcgg aagatctgat
     7921 cgcgaccgaa gcgttgcgcg tcggactagg tgtagtcgga cccacaacgc gtctcgccgg
     7981 atgatgatgg atggaaggag gtttcgccct acattgaggc gctgatcgaa agggaagaaa
     8041 aagccaagta gaagccaaat agaagacaga tagaatgcaa ataagcaaag gggaatgaca
     8101 ttatgtggaa acggcccata attcagagta tctgcgctgc aattcttgcc ataggcaccg
     8161 gggtcgctgc cagtgcgcag gactatcctt cgaaacccat caaactcgtc gtcgggtttg
     8221 gtgccggcgg tatgaccgac acctcgtcac ggatgatagc ggaggaactg gacaagaagc
     8281 ttggaacgcg catcgtagtc cagaaccagc cgggggttgg tggtcttctg gcgctggatg
     8341 ctgtgaagcg cgaaccggcg gacggctata ccatcgtaac ctttctttcg gattcgccgt
     8401 tcaccgccgc ctaccagggc aagtctttga atttcgacga ttggaccatg ctcggcggct
     8461 acatgcccca ggagcgcgtg ctatttggcc ggcaggacaa tcccttccac tctctcgatg
     8521 agctgatctc ttatgcgaaa aaaaacaatg ttacctttgc tgatggaggc tcattttggt
     8581 cggcgcgcgt gatggaggcg ttcgcgaagc agaacggcct caaaatgcgc ctcgtgcctt
     8641 tcaaatccgg cgcggaaggc tcagccgcta ttctcggcgg acatgtcatg cttgctgaga
     8701 cgggcgtcgg aactccagcc tggacttcgt ccaaagataa aggtcttaag atcctggcaa
     8761 ctctgacacc gggcggcctt gccgattttg ggcttccgga tgtgccgacg ctgaaaagtg
     8821 ccggtgcaaa ctttgtcgtg gacttctatt acggcctagc tatcaattcc aatacgcctc
     8881 ctgagcgcgt taaggtacta cgcgcggcgc tccaagaggt tatcgaggat cccgcggtcc
     8941 aggagcgatt tcggaaaatt gatctggtcc tcaagtggat gcctggtgac gcctatcttg
     9001 aaagaatgaa ggaggtcgcc aaatccgctg ccggactcaa agattacctg cagaaatgag
     9061 ctgacattca ctggggggag gatgtggttt tccctccagt gaaggcaaac ttgggcgaag
     9121 tgcgcctcgc ggaagcgtgc ctgtcgtgtg gcatcttgga aaagacgatt agatcctttc
     9181 acgggccccg gcagtagatc agctcttatc ccgcgcagca gggtggaagc cgcgcacgcg
     9241 agcctgccca cttccccgcc cggcctccaa accaagactt ttactttcca gcagatctcg
     9301 aaaggacctg tgaatgccgt tctctcacta cctgtatgat cgtctcggcc gggcggaaat
     9361 ctcgccaaaa ggtccgcacg tagcgggatc gcatgttccc ctggtgctca cctacactgc
     9421 gggcgtcgtt ggcattgacg atagcggcgc cctgaaattc agctggcgca ccgcttcaga
     9481 ttcgggaaaa ccgcagttca cagatcctcg tgcgcccaac tacaccacag cctatgccac
     9541 caacggcgcc aagctgcaca ttgaatataa ccgcaacaat attcgccctt gggtcaacac
     9601 cctgtttgtg cgggtggtcg ccggatttct gcgtgaaggt gaccaaatca ttgtgaaact
     9661 cggagacccc tcgcacggtt cgccggggta tcgcttacag acgtgttgcg atgatgattt
     9721 ccaattcaag acgttcgtcg acgcgttcgc cacgtatgat ttcgttccga tccctctgtc
     9781 gcccgcaata aagctgattt caggcaaacc cgcgcgctgg aaagcctatt tgccgaccct
     9841 acggcgcacg cgcgatgcat tccgactggt gattgttcct gaggaccgtt ggggcaatcc
     9901 gtcggaccag gctgatcaga cgatcctgct ttcgtcgaac atgccagtag aggggttacc
     9961 cgaccgcgta gtgttcgcca ggggcgacgg tcctcgggtg atagatggcc tcagagtcga
    10021 caaggctggc gtactcgaga ttagcctgat gggagccgac ggctcgccgc tcggttcatc
    10081 caatccgttg cggctcgcgg aggatgtccc gatgctccac tattggggcg atctccacgg
    10141 tcagagcaat gaaaccgtgg gcaccaatac cgttgaacag tatttcgctt tcgcacgaga
    10201 tcgcgcattc ctggatatcg tggcgcatca gggtaacgat tttcagattg atgatgagct
    10261 gtgggccaag atcaacgatc tatcggcgca atatgacgaa ccgggccggt tcgttgcact
    10321 cccggcgtac gaatggtcgg ggaataccgg aatgggcggc gaccacaacg tctactatcg
    10381 cgcggaagga aggccgatct accggtcgtc caaagtgctc atcagctcgg aaacgaaaga
    10441 tccggtttgc ctcaacgtta acgagctctt cgcggcactc gaaaaggagg acgcagtcgt
    10501 cattgcccat gtcggagggc gttacgctga catctccatc gggcattccg ggcgcatcca
    10561 acagtcggtc gagatccatt cttgctgggg aacatttgag tggctgctcc acgacgcgtt
    10621 tgatctgggc taccgcgttg gcctcgtctg ccacagcgac gatcacaagg ggaggccggg
    10681 cgccgcatat ccgggcgctt cgacgttcgg cgcgatcggc ggcctgacgt gttacatcat
    10741 gcctgagctc aaccgcgaca atttattcga ggcgctgcgt gcgcgccgtc actatgggac
    10801 gaccggcacc aggctctacc tcgacgtgaa agcgcagttt gatggcatcg ccgaacgctt
    10861 cgagacagac ccggcgcttg ggcccacgca gagcaaatcc gttcgtgccg cgaccatggg
    10921 cgacatcgtg cgtacaaccg acagctcggt taaactcgct gttgaggtca tcggttcggc
    10981 gccaatagaa cgggtgacta tcttcaacgg aaagtcgcca atcgccgttc accggaattt
    11041 cgactccagc gagctagggc atcgtatccg tgtcctttgg gagggtgccg agtatcgggg
    11101 acgggctcgg gaggtttact ggcgcggcca ctgccggctg gagggaaatc gctttacgcg
    11161 caccgaatcc ttcaatctgt tcaacgttga taagcctcta cgcgtggctg aggatgggac
    11221 cagggtggat ttcgattccg ttacgaccgg aaacttcgcg ggatttgacc tctggctgga
    11281 cgacagggaa gctggggaat tgatattcgc aagcgacgtt ctcgatgcgc gagtaccaat
    11341 cgcggagatc gggatggagg ataaggtcgt tgacgccggt gggctgggca agcgcctgcg
    11401 gatcttccgt ctcccggacg tcaacagcag tcgccagctt tcattcaccc atcacattga
    11461 actaagaagg gatagggata atccgatcta tgtgagggtg acacaggaag atggtcacca
    11521 agcgtggtcg agcccaatct atctcatccc ataaccagga agccccgagt tcgcgccgcg
    11581 caatccggcc agggcatgag gaggaggccc gttgaaggat caaaaatcgc tatttgcggg
    11641 agggatattc gtggccgtgg gctgcatcta tggcctcacg gcctggtggt cgcttccggt
    11701 gggcgactcc gtcaacatgg ggccaggata ctttccgatc gtgctgtcaa tgctcctcgt
    11761 attgctgggg gctgcaatcg ccgtgcgcag cgtggtgggg gagtacagcg tcgccttcga
    11821 aggaatcccc tggcgatcaa tcgctatgaa cacccttgcg atcctttgct ttgcctccct
    11881 aatcaggccg ctcggattat ttcccactgt gatgttgacg gcttctctta gctgtcttgg
    11941 gagcgccaat gtttcgatga cgaaagcagc cattgccggc ttctcaatcg ctgttctatg
    12001 cacgttgatc tttagtttca ttattggact gccgatcgcc atcgttggcc cctggctcgg
    12061 aggtttcatt tagataatgg agctcctcac tcatctcgcc atcggcttcg ccacagcgtt
    12121 ctcgccttcg aatttgctct attgctttct gggagtgcta ttggggactg tgatcggcgt
    12181 ccttcccgga atcggcacgg cgaccacggt ggccatgctt ctgccgctca catttcagtt
    12241 ggagccgacc tcatcgttga tcatgctcgc gggcatttac tacgggtccc agtacggtgg
    12301 atcaacgact gcgatcatgg tcaatttgcc tggcgaagcc tcgtccgctg tgacaacgat
    12361 cgacggctac cagatggcca ggagagggcg cgccggcccg gcgctggcgg ttgcagcgct
    12421 tgggtccttc ttcgccggaa cggtagcgac ccttttggtc ctcacggtgg cgcagcctct
    12481 caccgccata gccctgcgct tcggtccggc agaatatttt tcgttggtca ccgtcgggct
    12541 cgtttcctcc gtagcgctgg cgtccggctc gatcctcaac gcgctggcaa tgatttgcct
    12601 tgggcttctc atcagcatga cgggtacgga catctacacc gctatgccgc gcctgacgtt
    12661 cggcttgccg gagctcgaag gtggtattga tgtcgctgcc atcgcggctg gggccttcgg
    12721 catcggcgaa attatccgta attccgaagc aggcgtcatc gccaggagta ccattgctag
    12781 cgttaagagc atctggccat ctatagaaga cctcaagaga atggtcggcc caacggtccg
    12841 gggcaccacc atcggatcct ttctcggcgt ccttcccggg agcggagcgt tgctctcgtc
    12901 cttcgttgcc tacaacgtgg agaagaaggt ctcgcgcaat tctgctgaat tcggcaaagg
    12961 cgcgatcgag ggagtcgcag ctccagaggc ggccaacaat gcgggagccc aaacatcgtt
    13021 tatcccgatg ttgtcccttg ggctgccgtc gaacgtcgtc atggcgctga tgattggtgc
    13081 gatgatgatg cagggcatta cacccggacc caatgtcgct acgacacatc cagaattgtt
    13141 ctggggggta atcgtgtcga tgtggatcgg aaacgcaatg ctcgtgctgc tcaacttccc
    13201 gcttattggg atctggatca agctgttgag cactccttct tggatccttt atccagcgat
    13261 cgtcacattt tgctgcatcg gcatttacag ccttgccaat aattccttca ttatgtacat
    13321 cctcctcgta tccggtgtag gcggctacat cctggtgaag ctcggctgcg agctgcctcc
    13381 gtttattctc gggttcattc tcggtccaat gctggaggag catttccggc gcgcgatgag
    13441 cctttcgggg ggcgacccga tgatacttgt cacccgacca atcagcgctg ccctactcgc
    13501 cgtcgccgta gttgcgctta tagtcgtcag cctgccggcg atctcacgca agcggaagga
    13561 agtgttcgtc gaagaggatt gagccgccaa accttggacc ggctcgtcgt gaaggggtat
    13621 gccgatagcg ggattattcg cgtgtcctcg ccatctctgt agctcttgaa agcaagtgca
    13681 cccagggtca agtcctgctg ggatcgccat taggattaat gcgcttgaca tgtcctgttc
    13741 ttaagcgcca gcgccagtcc ggctcttttt ctcacttcgc ttttaaaggc gggcggcgca
    13801 gccttgcagc ctagaggaga aagcgagggg ctatgacgag ggctagtacc agtgaagacg
    13861 atgtcatctc agtctattgg cagacggggt gcacaagctg cctccggacg aaggaatttc
    13921 tgacccggca tggtgttgct ttccgctcgc ggaacgtgct ggaggatcag gaggctctcg
    13981 acgagctcgc tcgctttgga ttgcgccggg tgccgatcgt cgtgcgcggc gacaagtggg
    14041 cggatgggca agtcctccgg gacgtcgcga agctgtgcgg cattgcctgg gggaacgaga
    14101 aaatcctgcc tcccgatgag ctatgccgcc gcgtaaacgc aatcatcaaa gcgacgcagg
    14161 ccaacgtcct tcatctgccg gaagactcga tgcagacaca gttgcccaat cgaccacgca
    14221 gctatgccga cctcgtctat catatcttca acatcgtaga cgccttcgtg gagcacgaag
    14281 aaggcattcc gctggcctat gaggcctact atcgcgtgcc gcccgaagaa atgcgaaccc
    14341 cgaaaggcct agccgagtac ggcgagaagg ttggcgagcg cttcaacggc tggtttgccg
    14401 agcacggcga aactgcggat tgggcacgca aggcggacgt ctattacggt cagcaatcac
    14461 ttcatgaatt tctggagcgg accacatggc acagcggcca gcatgcccgt cagctcgtct
    14521 gggtgctcga gcgcatgggt atcgccccgg cgcagccttt gcatagcgaa gtgttccgcg
    14581 gcttacccat gccagagaag gtgtgggacg acgaacctgt gacctcctag cgaccgtcga
    14641 ttccgacagg gaaatagcgc tcaagcgaat ttgcggattt tcgcgataag ctcgagcgga
    14701 gagcgggtgg tccgccctcc gcccaaagtg cgcctctaac ggcgatgtcg gcggcgctcg
    14761 tgcctgtgct tgcgatagtg ccggtggccg cgtcgatcgt tgtcaccgaa gtagagtgtg
    14821 atgcccgggg ccgggtagcg atagaaccgg gggccacagc gcaccgtgcg cccgcgccgc
    14881 acatgccggt gcctccaagc tggtgcaccg cggcaggaac ggtggaaccc gtgcaccttc
    14941 tcgatctggg gctgtgcttt cactggagca ggcgaagccg gcagcgcatt cgcactcatt
    15001 gatgcgccgc ataggaccga tcccgctaag atggtcgctg agaacaagct tttcatagat
    15061 cctccttgac agctctcggc gtaatcactc cgtcaacccg ggctggaaag cgcgggttcc
    15121 tcaggtgcga ccgatctggc caatgaaaat ctcgaaacgc ttgcgaaatt ggagggatcg
    15181 ttgtcagaaa agctaagtat tagcaatgtt tactgatatt tgttcaagtc gataccgatg
    15241 tttcgggaaa tttcgtgcag taagaaatca gaattgctca ctgacagcct atctccggga
    15301 agtgtggtgc cgtcacgggt gctggcaggg atcggcaatt ccaccctaca catgcgccgc
    15361 gaaccgccga tcgcaggcgt gcgacttcgc gggctgtgga tcgtcggctg gggctacttg
    15421 ctgtttgccg agattccagc catctcgacc tgggtcggaa cagcgctgat cattcctcga
    15481 cagcgtccat cacttggcgg gaatcccgcc tcgcgtcgag gcgtaaacgc aaaccgcgtt
    15541 tatgagctgc tcattaaaca gtttccacgc gttgcggcct cgtgcatcat ctcgagggtt
    15601 ccgtctggcc cccagctaac ggcgcttgca tcgccaacag acctggaggt ttaccggcat
    15661 ggtggagtct cggcgcactg caccactcga aacgatcctc gagcacttgt cgctgcggcc
    15721 cgctaccggc tgcgaggatc tcatcagttt caagtcgagc ggtcagttga cgctgggggt
    15781 cgaaattgag ctgcaactcg tcgaccggac taccctgaac cttgccccgc gcgccgagga
    15841 agtgctggcg tgcgccggcg atctgcccaa cctgacaaag gagttctatc tcagcaccgt
    15901 cgagatcaac tcgcgcaagt gcgccgatgt gcatgaaatc agtagcgatc ttcacgccat
    15961 cctggagcgg gtggacgaga tcggcgatag gcttggcatt tctttctcaa cgacaggatg
    16021 ccatccgttc gcgcgctacg ccgactgcat cataacgccg acgccacgct ataacgagct
    16081 tatcgaccgc aaccaatggc tgacgcggcg catgaccgtc tacggcatgc acgtgcatct
    16141 cggcatgagt agcggcgacg agtgtatccg cttcaacaac ttcttcttgc atttcctgcc
    16201 acacctgctg gccctgtccg ccagctcgcc gttttggcaa ggcgaggata cggggctcgc
    16261 atcgtgccgg ccgaccacct acgaagcgct gccgacggcc ggtcagccat acgtggtcca
    16321 ctcctggcgg gagttcgggc aactctgcag cacgctgaga gcgtgtgatg ccatccaatc
    16381 actgaaggac ttgtggtggg acctgcgccc cagccccgcg cacgggacgc tggagatccg
    16441 catttgcgac ggcctggcga gcttgtcgga aacgctggcc gtcgtcgcgt tcgtccatgc
    16501 gctggcgcac tggttcggcg atcatgcgac ctggctggag aaggtgccgc cgccgccgcg
    16561 ctggctggcg cgcgagaaca aatggcgggc cgtgcgcttc ggcctcgatg ccaagctgat
    16621 caccagcctc gacggcacgg tcaagccatt gcgtgacgat gtgctcgact ggtgcgagcg
    16681 gatcgccggc aacgtggctg cgctcggcta cgagccctat tttgcgacgc ttcggcagat
    16741 tctggctgac ggcaacagct cgatgcgtca acgggcggtc tacgcccgca caggctcgct
    16801 ggaacaggtc gtgcagcaga atatcgccga attccgcgcc cgcgcgccgc tgtgggataa
    16861 aagccccctc gagagcccgg tacggtcgca gctcgagttc tccttggatc ggacccacga
    16921 cggaccgtga caggggcctg cctgatgctg ctgcgaggct caggacaagt ttgctgcgcg
    16981 atcttggaac tgtccgtcgt ctgcgtcatt ctatccccgg aacaagtcga gagcgaccgt
    17041 ccctaacatc gctcatccca tagctgccca attccggccg cgaaagtgcc ggcagtcgca
    17101 tgcatacgga agaatggtgt gagagggaga cggacgtgca ctgcagccgc ggctccagtg
    17161 catcgatact cccggctttt tgcggtggtg cggggtaatc ggtcatggat ttcgtggcgg
    17221 gtgcggacag cgtgcttgcg gccgtggggg cgctgggagc cgacagttgg cagaatgcag
    17281 ccgctttcgc tgcagtcctt gcggtcatcc tctatgctct gcgtgcaaac ggctttcttg
    17341 cccgggtctg gcgagcggtc caggacacaa tcctcaccaa ctggcagctc gcgcttctcg
    17401 gcgccgcggc catcgtgctg tcactcgcct ccggctggcg cacctgggat ggcatgcgca
    17461 acttcaccgg ggagtcggcg ctgtccctga tgatcacatt cggcattcag gccgtgatgc
    17521 tgatcgtcgc gtggctcatc ggtgaaagct tcgccaccgg catgagccag caggtagacg
    17581 gcgccaagcg cattcggaag cccatggatg gcgcgatcgg catggtgctg gggctcgcga
    17641 tctccggggc ggctttctac tggctcttgc atgtcttcgg cgcggtgagc tggaccagaa
    17701 cggccgattt ggccttcgac tggagccggt tcgcgaccat cgcgatgtat tttgccatcg
    17761 cattgctggt ggtcggggcg ctggccttca gcttcagccg cggcggcgaa ctcgcggtac
    17821 cctatgtgcg cgggacacgc gtcatcgcgc gcaacgtcgt gctgtgggtg atgctcctgg
    17881 cctgcatggg cacctcggtc ttcttctcct tcgactcatt tttcagctcg atcttccccg
    17941 aggatcagcg ccggcgctcg gcggaattgc gggcgcagaa ccaggtttcc ggtatcctgg
    18001 ccgatacggg cgcgacgatc gcgtctcgcc gggtcaccga agcagcccgc ttgttcgaga
    18061 ccgccgaatg gaaggcctac gatgcccagc tcaccgagct tgcgcagctg gcccagtcct
    18121 cttcgagcgc gctcaaggcc tatttccttc aggagctgga gcagcgccgc cgtctggtag
    18181 cggagcaact cgagcgcaag gccaccgccc agagcgggca ggccggtctg ctgacccgca
    18241 aaacggcgct catcgaagag ctggcgcgcg cccgcagcga gcggcccggc ctcgcggcgg
    18301 aatatgctca gcacaagagc gagctcgacg ccaaggcgcg cgaggtcgat gcaaagcggg
    18361 tcgaggcgat ggccgaggcg cgcggggttg aggggacggg caaagaaggc cggggaccgg
    18421 tctatcgcca gcgcatggcc gaactcgatc ggcttcaggg cgaatacgat atcaagctgg
    18481 agcg
//
LOCUS       DASVDU010000140         6275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_52824, whole genome shotgun sequence.
ACCESSION   DASVDU010000140 DASVDU010000000
VERSION     DASVDU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52824"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VF226_08955"
     CDS             complement(<1..580)
                     /locus_tag="VF226_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7074155.1"
                     /translation="MYLAGTSYTEVYSTFRWGIPPRFNIAEAVCSRYARTSPDAPALI
                     YEQDDGSVRVWSFGEVERAANRFANALAHLGVERGTIVGIHLPQCPESLISHVAVQKL
                     GGISLPMFNLFGPDAIEYRLRDSGARVLITTSGGLERLADSLKGIETLAHVISVGKSA
                     TGSAQVFWDLLEQASDAERATDTGPDDPAILMY"
     gene            complement(597..899)
                     /locus_tag="VF226_08960"
     CDS             complement(597..899)
                     /locus_tag="VF226_08960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX7074156.1"
                     /translation="MYVVAVFFESRKEHTAAFRAALLAQARNSLENEPGCRQFDVAQD
                     PLDPNTFFLYEVYDDEAAFKLHCETPHFKAFNDTVTPMTASKRVLTYELISGHGQA"
     gene            complement(920..1858)
                     /locus_tag="VF226_08965"
     CDS             complement(920..1858)
                     /locus_tag="VF226_08965"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7074157.1"
                     /translation="MSAVTIVEVAPRDGFQAVKEFIPTETKIRIVEALAATGVPRMEI
                     GSFVSPKAVPQLVDTGEVLKRARLPESMRVHVLVPNRKGLELAKAAGVREVGWVLSVS
                     ESHNQSNVRRTVDQSFADFEQAWAELGEGMRLRFSFATSFDCPFEGRTPESRVIACVE
                     RLLRIVPEVEIGIADTTGRAAPNHVRGLFTILVRDYASDKVELCFHGHDTYNLGVANA
                     IAAYEAGVRIIDGAAAGLGGCPFAPGATGNTATEDLVFAFEHMGISTGIDFAKLLEAA
                     DLTQTVAPEQAGGRVRTVSRKRALSGFGADSHGVAA"
     gene            complement(1855..3078)
                     /locus_tag="VF226_08970"
     CDS             complement(1855..3078)
                     /locus_tag="VF226_08970"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7074158.1"
                     /translation="MSDAIQPLLRSLRVLELGHFIAAPFCTRVLADLGADVIKLEPPG
                     GDPVRGWGMQAGGRSIWWSVHGRNKRCITLDVRKPEGREIVLDLVQKCDVVVENFRPG
                     QLEKWGLGPEELAKARPGIVLVRISGYGQTGPDAHKSGFGVIGEAKGGLRYIGGYPKD
                     VTDLPPPRAGVSLGDSVAGLYGAIGALAAVLDQRSSGAKEARVIDVALGEAVLTLLEG
                     ALPEYGLTGKIREPMGSAINTAAPTNAYPCADGTWLLIAANSDPLFAALCRVIDRPDV
                     AADPRFKDNRGRVEHVAELDAIISEWTRKLSSEEALERLEAANIPATKIYTIADVAND
                     AQFRARRMVTSVEDPLHGTLLHPGVVPMVEGLDRDAQIRWTGPAVGHHNSEVYEQLLG
                     YSRSRIAELREKGIL"
     gene            complement(3231..4031)
                     /locus_tag="VF226_08975"
     CDS             complement(3231..4031)
                     /locus_tag="VF226_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7074159.1"
                     /translation="MSSHPQSVGEHLREWRQRRRMSQLDLALAANVSARHLSFIETGR
                     ARPSREMVLHLCERLGVPYRERNSCLLAAGYAPVFPQRSLDDPAMIHARRAVDLVLAG
                     HEPYPALAVDRHWQLVAANRAVAHLIAGASDELLAPPVNVLRLSLHPRGLGSRILNYR
                     EWRDHILSRLNQQIDASADPVLIALCEELSSFPGPAERRSSAAGLLPNAVVTPLRLKT
                     DAGILSLFSTTTVFGTPVDVTLTELAIEAFFPADAETGERLRALSARS"
     gene            4170..4571
                     /locus_tag="VF226_08980"
     CDS             4170..4571
                     /locus_tag="VF226_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074160.1"
                     /translation="MPQIIRSLSLRRVLLLDTIATGATALLLLMGAEQLASLLGLPIA
                     MLRESGLILAAFVVFVGWMAAQEPAPISAVRLVIAANVIWVAASLMLLVSGFVEPTAA
                     GYVLISAQALVVGGFAELQMMALRRSRVATA"
     gene            4594..4932
                     /locus_tag="VF226_08985"
     CDS             4594..4932
                     /locus_tag="VF226_08985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX7074161.1"
                     /translation="MQPRDAAQRTGVAVTVEIRVKGGHDEEFLQLLTPVLDEMRHETT
                     FINAILHRDPEDPTRFMLYETWADLNELAEVQLQRAYRKAYIDRLPAILREERKITIW
                     QPLRSDSSSG"
     gene            complement(4983..>6275)
                     /gene="gdhA"
                     /locus_tag="VF226_08990"
     CDS             complement(4983..>6275)
                     /gene="gdhA"
                     /locus_tag="VF226_08990"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX7074162.1"
                     /translation="AGEVEFHQAVREVLESIGLVIAKRPEYADQALIERICEPERQVI
                     FRVPWFDDRGQVQINRGFRVGFNSALGPYKGGLRFHPSVNLGIIKFLGFEQTFKNALT
                     GMPIGGAKGGSDFDPKGRSEMEVMRFCQSFMTELYRHLGEYTDVPAGDIGVGQREIGY
                     MFGQYKRITNRYESGVLTGKGLTWGGSQVRREATGYGAVFFVEQMLKTRGLDFDGKKV
                     VVSGSGNVAIYTIEKIQELGGRVIACSDSDGYVVEEGGIDLELLKEIKEVRRERISVY
                     ARLRKDTADYVPKGRIWDVPCEIAMPSATQNELNGGDARKLVKNGVIAVGEGANMPCT
                     PEAVRIFQDANVLFGPGKATNAGGVATSALEMQQNASRDSWTFEETEQRLATIMRQIH
                     DRCAETADEYGMPGNYVAGANIAGFIRVAEAMQALGVV"
BASE COUNT         1093 a         2098 c         1973 g         1111 t
ORIGIN      
        1 tgtacatcag gatcgccgga tcatccggac ccgtatcggt cgcgcgctcg gcatcgctgg
       61 cctgctccag cagatcccag aagacctgtg ccgagcccgt tgctgatttc ccgaccgaga
      121 tcacatgcgc cagcgtctcg atccccttga gcgaatccgc cagccgctcc agcccgccag
      181 acgtggtgat gagcacgcgg gcgccgctgt cccgcaggcg gtactcgatg gcgtccggcc
      241 cgaacaggtt gaacatcggc agcgagatcc cgccgagctt ctgcacggcg acgtggctga
      301 tcaggctttc cgggcactgc ggaagatgga tgccgacgat cgttccgcgc tccaccccga
      361 ggtgagccag cgcgttggcg aagcggtttg ccgcccgctc gacctcgccg aagctccaga
      421 cccgcacact gccatcgtcc tgctcataga tcagtgccgg agcatcgggc gacgtccgcg
      481 cgtatcggct gcacaccgcc tcggcgatgt tgaaccgggg cggaatgccc caccggaacg
      541 tgctgtaaac ctcagtgtag ctcgtgccgg ctagatacat tcgaccgtag tccccatcag
      601 gcctgaccgt gtccggaaat cagctcatag gtgagcacgc gcttggaggc ggtcatcggc
      661 gtgactgtgt cgttgaacgc cttgaagtgc ggcgtctcgc agtggagctt gaaggccgcc
      721 tcgtcgtcgt aaacctcgta aaggaagaag gtattgggat cgagcgggtc ctgcgccacg
      781 tcgaactgac ggcagcccgg ctcattctcc aggctgttgc gagcctgcgc gagcagggcg
      841 gcacggaagg cggccgtgtg ctccttccgc gactcgaaga acacggcaac gacgtacatc
      901 gaatgctccc ccgggcgatc tatgcggcaa cgccatgcga atcggcgccg aaccctgaaa
      961 gggcgcgctt gcgcgagacg gtccgcacac gtccgcctgc ctgctccggc gccaccgtct
     1021 gcgtgagatc ggcagcctcg aggagcttgg cgaagtcgat ccctgtcgaa atccccatgt
     1081 gctcgaaggc gaacaccaga tcctcggtcg ccgtattgcc cgtcgcgccc ggtgcaaatg
     1141 gacagccgcc gagtcccgcc gcggcgccgt cgatgatccg tacacctgct tcgtaggccg
     1201 caatcgcgtt ggcgacgcca aggttgtacg tgtcgtgtcc gtggaagcag agctcgacct
     1261 tgtcgctcgc ataatcgcgg acgaggattg tgaagagccc gcgaacgtgg ttcggggccg
     1321 cccgtccggt ggtatcggcg atgccgatct cgacctcagg cacgatccgc agcagccgct
     1381 cgacacaggc gatgacgcgc gattccggcg tgcggccctc gaacggacag tcgaagctgg
     1441 tcgcaaagct gaaccgcagc cgcatgcctt cacccagttc cgcccaagcc tgctcgaaat
     1501 cggcaaagct ctggtcgacg gtccggcgga cgttggactg attatggctt tcgctgaccg
     1561 acagcaccca gccgacctcg cgcacgcccg cagccttggc gagctccagt cccttgcggt
     1621 tcggcaccag cacgtggacg cgcatgctct cgggcagccg cgcccgcttc agcacctcgc
     1681 ccgtgtcgac gagctgcgga acggccttcg ggctgacgaa ggagccaatc tccatgcggg
     1741 ggacgccggt tgccgccagc gcctcgacga tgcggatctt cgtctcggtc ggaatgaact
     1801 ccttgacggc ctggaagccg tcgcgcgggg cgacctcgac gatagtgact gcgctcatag
     1861 gatccccttt tccctcagct ccgctattct ggatcgagaa tagccgagga gctgctcgta
     1921 gacttcgcta ttgtggtggc caaccgccgg gccggtccag cggatctgcg catcgcggtc
     1981 gagtccctca accatcggca cgacgcccgg atgcagcaag gtcccgtgca gcgggtcctc
     2041 gacgctggtg accatgcggc gggccctgaa ctgggcgtcg ttcgccacgt cggcgatggt
     2101 gtagatcttg gtcgccggga tgttcgcggc ctcgagccgc tccagggcct cttcgctcga
     2161 cagcttgcgc gtccattcgg agatgatggc atcgagctcc gcaacgtgct cgacacgacc
     2221 gcgattgtcc ttgaaccggg gatcggcggc cacgtcgggc cggtcgatca cacgacagag
     2281 cgccgcgaaa agcgggtcgg aattggcagc gatgaggagc caggttccgt cggcgcaagg
     2341 atacgcattg gtgggggccg ccgtgttgat cgccgagccc atgggctccc ggatcttccc
     2401 tgtgagcccg tattcgggca gcgcgccttc gagcagcgtc agcaccgctt cgcccagcgc
     2461 cacgtctatg acgcgcgctt ccttggcacc tgagctgcgc tgatcgagca cggcggccag
     2521 ggcgccgatc gcgccataaa gaccggcgac gctgtctccc aggctcacgc cggctcgcgg
     2581 cggcggcaga tcggtcacat ctttcgggta gccgccgatg taacgcagcc cgcccttggc
     2641 ctcgccgatc acgccgaacc cggacttgtg cgcatcgggc ccggtctggc cataaccgga
     2701 aatccgcacc aggacgatgc cggggcgcgc cttcgcgagc tcctccggac caagccccca
     2761 cttctcgagc tggccggggc ggaagttctc gacgacgacg tcgcatttct ggacgaggtc
     2821 caggacgatc tctcgcccct cgggcttgcg tacatcgagc gtgatgcacc gcttgttgcg
     2881 gccgtgcacg ctccaccaga tggaacgccc gccggcctgc ataccccagc cgcgcaccgg
     2941 atcgccgccg ggcggctcca gcttgatgac gtccgcaccg aggtcggcga gaacacgcgt
     3001 gcagaaaggg gccgcgatga agtgaccaag ctcgaggacg cgcaggcttc tgagcagtgg
     3061 ctggatggca tcggacaccg gcagcgctcc taatcacccc tcggaatgga tccgggctgg
     3121 cgggaaaatt ctgtgccagt aatagtcaat cgtcagacgg gttgccaccc attcaggcca
     3181 actccttctg agggcttcat agcccctcgc gtcggcgtca caccccgctg ctagctcctc
     3241 gcgctaaggg cgcgcagcct ttcgcccgtt tcggcatcag cgggaaagaa tgcctcgatc
     3301 gccagctccg taagcgtcac gtcgaccggc gtcccgaaca ccgtcgtggt gctgaagagc
     3361 gaaagaatgc cggcgtcagt cttaagtcgc aggggcgtca cgacagcgtt cggaagcagg
     3421 ccggcggccg agcttctgcg ctccgctggg ccagggaagg acgacagctc ctcgcagagg
     3481 gcgatcagaa ccggatcggc gctggcatcg atctgctggt tgagccggga caggatgtgg
     3541 tcgcgccatt cccggtagtt caggatccgc gatccgagcc cccggggatg gaggctgagg
     3601 cgcagcacgt tcacgggtgg cgccagaagt tcgtcgctcg cacctgcgat caggtgggcg
     3661 acggcgcggt tggccgcaac aagctgccag tgccggtcga ccgccagcgc cggatagggc
     3721 tcgtggcccg cgaggacgag atcgaccgcc cggcgcgcat ggatcatcgc cggatcgtcg
     3781 agcgagcgtt gcggaaaaac gggcgcataa ccggccgcga gcaggcacga attccgctcc
     3841 cgatacggaa cgccgagccg ttcgcacagg tgcaacacca tctcgcggct gggccgcgcg
     3901 cgaccggtct cgatgaagct caagtgccgt gcggaaacat tggcggccaa agcgagatcg
     3961 agctggctca tgcgccggcg ctggcgccat tcgcgcaagt gctcaccaac ggactgggga
     4021 tggctgctca ttcgcgaacg atacctgcgg ccaccgccta catccaatga cctgcgaggt
     4081 aattgcggcg gttctgtccc ctccctagcc tcgagaccat cgcaggccct acggggccgc
     4141 ggcgcaccaa tcagacggga cttaaagcca tgccgcaaat catcagatca ttgagccttc
     4201 gccgggtcct cctcctcgac acgatcgcca ccggggccac cgcccttctc ctgttgatgg
     4261 gtgcggagca actcgccagc ttgctgggat tgccgattgc gatgttgcgg gaaagcggcc
     4321 tgatcctcgc tgcctttgtc gtcttcgtcg gctggatggc agcccaggag cctgcgccga
     4381 taagtgccgt caggctggtc atcgcagcca acgtcatttg ggtcgccgcg agcctgatgc
     4441 tcctcgtgtc cgggttcgtg gagccgacgg ccgccggata cgtcctcatc agcgcccagg
     4501 cactcgtcgt tggcggattc gccgagttgc agatgatggc gcttcgtcga tcgcgcgttg
     4561 cgacggcttg acccgcttac caggaggttc gcaatgcaac cgcgagatgc tgctcaacgt
     4621 accggtgtcg ccgtcaccgt ggagatccgc gtgaaagggg gccacgatga ggaattcctg
     4681 cagttgctca ccccggtact cgacgaaatg cggcatgaga cgacgtttat caacgccatc
     4741 ctgcaccgcg atcccgagga tccgacccgc ttcatgctgt acgagacgtg ggctgatctg
     4801 aacgagctcg ccgaagttca gctgcagcga gcctatcgca aggcctacat cgatcgcttg
     4861 ccagcgatcc tgcgtgagga gcgcaagatc acaatttggc agccgttgcg ctccgattcc
     4921 tcaagcggct gagcccgcga gcccaggtgc gggctggtcg ttttaagccc gctccttgca
     4981 actcagacga ccccgagcgc ctgcatcgcc tcggccaccc ggatgaaacc ggcgatattg
     5041 gcgccagcga cgtagttgcc cggcatcccg tattcatccg ccgtctccgc gcagcggtcg
     5101 tgaatctgac gcatgatggt ggcgaggcgc tgctccgtct cctcgaacgt ccaggagtcc
     5161 cgcgaggcgt tctgctgcat ctcgagcgca ctcgtcgcaa cgccgcccgc gttggtcgcc
     5221 ttgccgggtc cgaagagcac gttggcatcc tggaagatgc gcaccgcctc cggcgtgcag
     5281 ggcatgttgg ccccctcacc tacggcgatc acaccattct tcacgagctt gcgggcatca
     5341 ccaccattga gctcgttttg cgtcgccgaa ggcatggcga tttcgcaggg gacatcccag
     5401 atcctgccct tcggcacgta atctgccgtg tccttccgca ggcgggcata gaccgagata
     5461 cgctcccgcc gcacctcctt gatctctttc agcagctcga ggtcgatgcc gccttcttcg
     5521 accacgtagc cgtccgagtc cgagcaggcg atcacgcgcc cgccaagctc ttggatcttt
     5581 tcgatcgtgt agatggcgac attgccagac ccggaaacga cgaccttctt gccgtcaaaa
     5641 tccaatccgc gggtcttcag catctgctcc acgaagaaga cggcgccata gccggtcgcc
     5701 tcccgccgga cttgcgagcc gccccaagtc aggccctttc cagtgaggac gccggactcg
     5761 tagcggttgg tgatgcgctt gtactggccg aacatatagc cgatctcgcg ctgcccgacg
     5821 ccgatgtcgc cggccgggac gtccgtatat tcgccgagat ggcggtaaag ctcagtcatg
     5881 aacgactggc agaaccgcat gacctccatt tcggagcgcc ccttgggatc gaaatcggac
     5941 ccgcccttgg cgccgccgat gggcatgcct gtcagagcgt tcttgaacgt ctgctcgaag
     6001 ccgaggaact tgatgatccc cagattgacg ctggggtgga acctgaggcc gcccttgtag
     6061 gggccgagcg ccgagttgaa gcccacccgg aagccgcggt tgatctgcac ctgacctctg
     6121 tcgtcgaacc acggaacgcg gaaaataacc tggcgctcag gctcgcagat ccgctcgatc
     6181 aatgcttgat cggcgtattc ggggcgcttg gcgatcacga ggccgatgct ttcgagcacc
     6241 tccctcacag cctggtggaa ctcgacctcg ccggc
//
LOCUS       DASVDU010000141         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_392800, whole genome shotgun sequence.
ACCESSION   DASVDU010000141 DASVDU010000000
VERSION     DASVDU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392800"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..444
                     /gene="rnpB"
                     /locus_tag="VF226_08995"
     ncRNA           21..444
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF226_08995"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(476..1717)
                     /locus_tag="VF226_09000"
     CDS             complement(476..1717)
                     /locus_tag="VF226_09000"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEX7074163.1"
                     /translation="MRSKPFTLGWAEWVALPDLGLPAIKAKVDTGARTSALDAFQIEP
                     FGPAEAPMVRFGVHPIPGRTDVVIYSSAPVIDQREVTSSNGEREVRCVIATRISIGER
                     TWPIEVTLTNRETMTYRMLLGRQAIRGDIQVDPATSYLQPKLSYRLYRHAPRHDLVHR
                     PLRIALLTRQPRSASNRRLREAAAARGHVLESLDPGRMSLVMDTMEPVLLAGNAPVGH
                     YDAVIPRPTGGDGMFDAAAVRQLELTGSYAVNPGDALDRLLNPIATRQKLMARSVPVP
                     VETVTTEAAASNKTARRTAPVLRLLVVGGEVVAVIERRLGREHDASARALKEERTIAI
                     AAAEALGLRLASIDIGEASTGPAVLHASAAPALGKFEAITGARVAEAVIADVETRVRS
                     WRRERPAGLTGLPNGQEPQRG"
     gene            1834..2352
                     /gene="def"
                     /locus_tag="VF226_09005"
     CDS             1834..2352
                     /gene="def"
                     /locus_tag="VF226_09005"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7074164.1"
                     /translation="MAKLPIITLPDPILREISAPIERVDDETRRLIADMFETMYAAPG
                     VGLAAVQVGIPRRLIVVDATEEGEERRPIAMLNPEIVSLGDEKRPYEEGCLSIPDVRV
                     EIERPASVHVRFLDAQGRLQELKAEGLLATAIQHEIDHLDGRLIIDFLSRLKRDIIVR
                     KFRKQAKGGVAA"
     gene            2396..3325
                     /gene="fmt"
                     /locus_tag="VF226_09010"
     CDS             2396..3325
                     /gene="fmt"
                     /locus_tag="VF226_09010"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7074165.1"
                     /translation="MRIVFMGTPDFAVPTLAEILAAGHDVVAVYTQPPRPAGRGMGER
                     KSPVHVYAESNGIPVFTPKSLRGEAEQEIFRSLDADVGVVVAYGLILPKPILEAPRYG
                     CLNLHASRLPRWRGAAPIQRAIMAGDTETAVMVMQMDEGLDTGPILLAERVPIGPNTT
                     AGELHDELARVGASLMVRALAALERGSLDATPQSEEGVTYAAKIDKAEARIDFTRPAR
                     EVHDLIRGLSPLPGAWFEAGPERRRERIKVLRSELADGDGPPGTVLDDRLTVACGEGA
                     VRLTLLQRAGRKPMSAEEFLRGFDLPAGTKLKK"
BASE COUNT          635 a         1186 c         1226 g          593 t
ORIGIN      
        1 cgcgccctta ttcacttatg ccagccggcc ggacggccgc tgccggtgat ctcgctttgt
       61 ggcgaacagg accggcagag gaaagtccgg gctccatggg aacacggtgg cggctaacgg
      121 ccgccggggg cgaccccagg gaaagtgcca cagaaagcaa accgccgaag gcggctcttc
      181 ggagcttgcc aggcaagggt gaaagggtgc ggtaagagcg caccgcgcga ctggcaacag
      241 gagcggcagg gcaaacccca ccgggagcaa gaccaaatag gggtggccgg gcgagcgggg
      301 gtgatcccgc ggcgccaggc ccgtttccag gcccgccacc cgggttggtt gcacgaggcg
      361 cgcggcaacg cgcgtcccag atgaatggcc gtcgcggagg agcagtcctc cagacagaac
      421 ccggcttaca ggccggctgg cacatgttct ccagcccgcg cttggcggga tccgatcagc
      481 ccctctgtgg ctcctgtccg ttcgggagac ctgtcagccc agcgggcctt tcgcgcctcc
      541 aggaccggac cctggtctcc acatcggcta tgaccgcctc ggccacgcgc gcgccggtga
      601 tcgcctcgaa cttgccgagc gcgggggctg ccgaagcatg cagcacggcc gggccggtgc
      661 tggcttcgcc gatgtcgatg ctcgccagtc gcaagccgag cgcctcggcg gccgcaatgg
      721 cgatggttct ctcctccttc agggcgcgtg cgctggcgtc gtgctcgcgg ccgaggcggc
      781 gctcgatcac cgccacgacc tccccgccga cgaccagcaa ccgcagaacc ggagcggtcc
      841 gccgcgcggt tttgttgctc gctgcagctt cggtcgtcac ggtctcgacc ggcacaggca
      901 cggaccgcgc catcaacttc tgccgggtgg cgatcgggtt gagcagccgg tcgagagcat
      961 cgccggggtt gacggcatag ctgcccgtca gctcgagctg ccgcacggcc gcggcatcaa
     1021 acatgccatc accaccggtc ggccggggga taacggcgtc gtagtgcccg acaggcgcat
     1081 tgccggcaag cagcaccggc tccatcgtat ccatcaccag ggacatccgg ccgggatcga
     1141 gcgactccag cacgtgcccg cgcgccgccg cagcctcccg caaccggcga ttggaggcgc
     1201 tgcgcggctg gcgcgtaagg agcgcaatcc gcaacggcct gtgcacgagg tcgtggcggg
     1261 gagcatggcg atagagccgg taggacagct tcggctgtag gtaggaggtc gcgggatcga
     1321 cctggatatc gccacggatc gcctgccgtc cgagcagcat gcggtaggtc atggtttccc
     1381 ggttggtgag cgtcacctcg atcggccagg tgcgctcgcc gatgctgatc cgggtggcaa
     1441 tgacgcagcg cacctcgcgc tcaccgttgg agctcgtgac ctccctttga tcgatgaccg
     1501 gtgccgagct gtagatgacg acatccgtgc gccccggaat ggggtggacg ccgaagcgca
     1561 ccatcggcgc ctccgccggg ccgaacggct cgatttgaaa cgcatccagg gcagaggttc
     1621 gcgcgcccgt gtcgaccttc gccttgatgg ctggcaaacc gaggtccggc agcgcgaccc
     1681 actccgccca gccaagtgtg aatggcttag atctcatggc aattgccggt gcgtacaact
     1741 cgtgtggacg acggcagtga ctgtaccagc ggccagaaac cgcgaggatt tcacgcgatt
     1801 gaccggctcg gcgccgtcgc ttatctcaca gacatggcaa aactccctat cattacgctc
     1861 cctgatccga ttttgcgcga gatttccgct cccatcgagc gagtggacga tgagacgcgc
     1921 cggctgatcg cggacatgtt cgaaaccatg tacgcggcgc cgggcgtggg gctcgctgcg
     1981 gttcaggtcg gcattccgcg gcgccttatt gtggtggatg ccaccgagga gggcgaggag
     2041 cggcggccga tcgcgatgct gaaccccgag atcgtctcgc tcggagacga aaagcggccc
     2101 tacgaggagg gttgcctttc gattcccgat gtgcgggtcg agatcgagcg accggcaagt
     2161 gtgcacgtgc gcttcctcga tgcgcaaggg cggctgcagg agctgaaagc ggagggactg
     2221 ctggcaacgg cgatccagca cgagatcgat catctcgacg gccggctcat catcgacttc
     2281 ctgtcgcgac tgaagcgcga catcatcgtt cgtaagttca ggaagcaggc gaaaggcggc
     2341 gtcgccgcct gacgcggacg cccgccgtag cacaagtgaa aggcgatccg tgccgctgcg
     2401 catcgtgttc atggggaccc cggacttcgc ggtgccgacg ctcgcggaga ttctggctgc
     2461 gggtcacgat gtcgtggccg tctacaccca gccgccgcgg ccggcgggcc gcggaatggg
     2521 cgagcggaag tcgccggttc atgtctacgc cgagagcaac ggcattcctg tcttcacgcc
     2581 gaagtccctg aggggcgagg ccgagcagga gatctttcgc tctctcgatg ccgacgtcgg
     2641 cgtggtcgtc gcctacggat tgatcctgcc gaagcccatc ctggaggcgc cgcgctacgg
     2701 ttgcctcaat ctgcatgcct cccgcttgcc ccgctggcgt ggcgcagcgc ccattcagcg
     2761 cgcgatcatg gccggcgata cagagacggc tgtcatggtc atgcagatgg acgaggggct
     2821 cgataccggg ccgatccttc tggcggaacg cgtgccgatc ggacccaaca ctacggcggg
     2881 cgagctgcac gatgaactcg ccagggtggg cgcgagcctg atggtgcgtg cgctcgcggc
     2941 gctggagcgc ggcagtctcg atgccacccc gcaatccgag gagggggtga cttacgccgc
     3001 caagatcgac aaggccgagg cccgcatcga tttcacccgg cctgcgcgcg aagtccacga
     3061 tctcatccga gggctgtcgc cgcttccggg tgcctggttc gaggcaggac cggagaggcg
     3121 gcgcgagcgg atcaaggtgc tgcggtcgga gctggccgac ggcgacggac cgcccgggac
     3181 cgtgctcgac gatcgcctca cggttgcctg tggggagggc gcggttcgtc tcacgctcct
     3241 gcaacgtgcc ggccgcaagc cgatgtcggc agaggagttc ctacgcggct tcgacctgcc
     3301 tgccggcacg aagctcaaaa aatagaagct gtttcaacga aatttacaac gcacactgac
     3361 tggcgaaccg ccgctgcgga ccgcaactct tgtggattcc ttctcgtcgg tgttgatcca
     3421 gtggatagcg cgtgcgagcg tagtgccaag tgagtctcga atcgcacagg tttgctcccg
     3481 aagctattgt ttgggagcca attgttttgc tcgaccaccc gaagctaccg cttaagctgc
     3541 cactgcagcc aaaaccgaac gccaaaatac ggcgcactcc gccatcgagc aagccgccaa
     3601 ccacgcctcc agaactggtg tcatcgccac tccggccgca
//
LOCUS       DASVDU010000142         3052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_581922, whole genome shotgun sequence.
ACCESSION   DASVDU010000142 DASVDU010000000
VERSION     DASVDU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581922"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="VF226_09015"
     CDS             complement(<1..1100)
                     /locus_tag="VF226_09015"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7074166.1"
                     /translation="MPTGPSFGQGAKELSDLDSAHGSNVSICRRTFGKGVAATTSALL
                     LMGSQAAKASGGFDELDAALDGYVANGSVPGLVALVARGDTVHVHAAGVQNVETDVPM
                     QRDTIFAVASIGKPITAVSALILVEDGVLGLDDPVDKWLPELADRRVIRSLDSALDDT
                     VPANRPITLRDLLTLRMGLGAVFADPSSSPLLQRMNELQVAPGPKLFGHSSDEFMRRL
                     GSLPLVHQPGERWLYHTGMDVAGVLIERASGMSLGDFQRKHIFEPLGMADTGFYVPKE
                     KAARLATCYWRDTETGKLTVWNPASGASFTEPPPFEAGGGGHVSTVEDYLAFGRLLLG
                     KGAYRGRRIISEESVAAMLTDQITAEQKAVSP"
     gene            complement(1205..2479)
                     /locus_tag="VF226_09020"
     CDS             complement(1205..2479)
                     /locus_tag="VF226_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7074167.1"
                     /translation="MVLLVASFLVLLLVGVPIFISLAASSLLYTHFVSGIPDFVVLHR
                     MAGGVDSFPLLAVPFFILAGNLMNSAGITNRIYDFAVAAVGWLRGGLGHVNVVGSVIF
                     AGMSGTAVADAGGLGTIEIKAMRDHGYKADFAVGITGASSTIGPIIPPSLPMVVFGVM
                     ANVSIGQLFVAGIVPGLLMAAFQMLYITWYARRHGIGRDQVFRWDVLGRTLVAAIPAM
                     MTPVIIIGGMSFGIFTPTEAAIAACAWALILGVFLYRSLTWKRFYKISMDTIETTAAV
                     LIIVGAASLFGWVLTTTQITQAVTDALLSISRDPLVLLLIINVLLLIVGCFMETIAAI
                     SILVPVLMPVVLAAGIDPVQFGVVMVLNLMIGLLTPPVGMVLFVLSRIANISFDRTVR
                     SILPFLIPLVLVLILISAFPWLTLAVPTYFYR"
     gene            complement(2491..>3052)
                     /locus_tag="VF226_09025"
     CDS             complement(2491..>3052)
                     /locus_tag="VF226_09025"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7074168.1"
                     /translation="GRNVVQQQGHPEEHLIHVEDAEVTIEHHAEDWVSFALFWALALI
                     VFLQFFTRYILNDSLAWTEEIARYFLMILTFIGAAIVARKGAHISVEVLLHFLPERGA
                     RILLAVIEVIQAAFIALLFYFSLTITQRMHAQRMTVFDEPMSLVYGPIAFGCLLMLVR
                     ALQRLWRNAKDGWRTAYASIGSSTVE"
BASE COUNT          656 a          905 c         1003 g          488 t
ORIGIN      
        1 aacggtgaga ccgccttctg ctccgcggta atctggtcgg tcagcatggc agccacgctc
       61 tcctcggaaa tgatccgtcg tccgcggtat gcgccttttc cgagcagaag gcggccgaat
      121 gcgagataat cttcgacggt cgagacatgg cccccgcccc cggcctcgaa cggaggtggc
      181 tcggtgaagc tcgcgcccga ggcgggattc cagaccgtga gctttcccgt ttccgtatcg
      241 cgccaatagc aggtcgcgag ccgggccgct ttttccttcg gcacgtaaaa ccccgtatcg
      301 gccatgccga gcggctcgaa aatgtgcttg cgctggaaat cgccgaggct cattccgctg
      361 gctcgctcga tcagcacgcc cgcgacgtcc atgccggtat gatagagcca gcgctcgccc
      421 ggctggtgca cgaggggaag cgagccgagc cggcgcatga actcgtccga gctgtgcccg
      481 aacagcttgg ggccgggggc gacctgcagc tcattcattc gttgcaggag cggagagctg
      541 gaaggatcag cgaaaacggc gccaagcccc atgcgcaaag tgaggagatc gcgcagcgtg
      601 atcgggcggt ttgctggaac ggtgtcgtcg agagcgctgt cgaggctgcg gatgacgcgc
      661 cggtcggcga gctcgggcag ccatttgtcg acgggatcgt cgagacccaa tacgccgtcc
      721 tcgaccagga tcagcgcaga tacggcggtg atcggtttgc cgatggacgc gacggcgaaa
      781 atggtgtcgc gctgcatcgg tacgtccgtt tcgacattct gcacgccggc ggcatggacg
      841 tgaacggtgt cgccgcgagc cacgagggcg accagccccg gaacagaccc attcgcgaca
      901 tagccgtcga gcgccgcatc gagctcatcg aaaccgcccg aggctttggc tgcttggctg
      961 cccatgagta gtagagccga tgtcgttgct gcaacgcctt tgccgaaagt ccgccggcat
     1021 atcgatacat ttgagccgtg ggccgaatcc agatcgctca gctccttcgc accttgcccg
     1081 aatgaaggac cggtcggcat aagatcacct ccgcctggca gtcgttatcg ataggctcgg
     1141 ctgggtagag ttgatatcaa cctaatccgg cactgtcaaa ctctgagctc ccaaaagggg
     1201 ggcgtcaccg gtagaagtag gttgggaccg cgagcgtgag ccaggggaac gcggagatca
     1261 ggatcagcac gagaaccagc ggtataagga atggcaggat gctgcgcacc gtacggtcga
     1321 aagagatgtt ggcaatgcgg ctcagcacaa agaggaccat gccgaccggt ggagtcagca
     1381 gcccgatcat caggttcagg accatcacca cgccgaactg gacgggatcg atcccggccg
     1441 ccagcacaac cggcatgagg accggcacca gaatggagat ggccgcgatc gtctccatga
     1501 agcagccgac gatcagcagc aggacattga tgatcaggag caggaccagg ggatcgcgcg
     1561 agatggacag cagcgcatcg gtgaccgctt gagtaatttg cgtcgtggtc agcacccagc
     1621 cgaacaacga cgcagcgccg acgatgatca gcacggcagc cgtcgtctcg atggtgtcca
     1681 tcgagatctt gtaaaaccgc ttccacgtca gcgaccggta gagaaagacg ccgaggatga
     1741 gcgcccacgc gcaggcggcg atcgccgcct ccgtcggcgt gaagatgccg aaagacatcc
     1801 cgccgatgat gatcacgggt gtcatcatcg caggaatcgc cgcaacgagc gtgcgtccga
     1861 ggacatccca gcggaacacc tggtcgcggc cgatgccgtg acgccgggcg taccaggtga
     1921 tgtagagcat ctgaaaggcg gccatgagca ggccggggac gatgccggct acgaagagct
     1981 gtccgatcga aacgttggcc atcacgccga agacgaccat cggcagagac ggaggaatga
     2041 tcgggccaat cgtcgatgag gccccggtga tgccgacggc gaagtcggcc ttgtagccgt
     2101 ggtctcgcat cgccttgatc tcgatggtgc ccaggccgcc ggcatcggcg acggccgtgc
     2161 ctgacatgcc ggcgaagatc accgagccga cgacgttgac gtggccgagg ccgccacgca
     2221 gccagccgac cgcggctacc gcgaaatcgt agatgcggtt cgtgatgccg gccgagttca
     2281 tgagattgcc ggccagaata aaaaacggca ctgccagcag aggaaaactg tcgacgccac
     2341 cggccatgcg gtgcagcacg acgaagtcgg gaatgcccga gacgaagtgc gtatagagca
     2401 gcgacgaggc ggccagcgaa atgaagatcg ggacaccgac gaggagcagg acgaggaacg
     2461 aggcgaccag aagcaccatg tccgtgcacc ttattccacg gtcgagctgc cgatgctggc
     2521 gtacgccgtg cgccagccgt ctttcgcgtt ccgccaaagg cgttgcagcg cgcgcaccag
     2581 catcagcagg cagccaaagg cgatcggtcc gtagacgagg ctcatgggtt cgtcgaagac
     2641 cgtcatgcgc tgggcgtgca tccgttgcgt aatggtgaga gagaaataga agagcagcgc
     2701 gatgaatgcg gcctggatca cctcgatgac ggccagcagg atccgtgctc cgcgctcggg
     2761 caggaaatgc aggaggacct cgaccgagat atgggcaccc ttgcgggcaa cgattgccgc
     2821 cccgatgaag gtcaggatca tgaggaagta gcgggcgatc tcctcggtcc aggcgaggct
     2881 gtcgttgaga atgtaacgtg tgaagaactg caggaagaca atgagcgcga gcgcccagaa
     2941 tagcgcgaat gatacccaat cctcggcgtg atgctcgatc gttacctcgg catcctcgac
     3001 gtggatcagg tgctcctcag ggtgcccctg ctgctgcacg acgttcctcc ct
//
LOCUS       DASVDU010000143         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_10883, whole genome shotgun sequence.
ACCESSION   DASVDU010000143 DASVDU010000000
VERSION     DASVDU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10883"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VF226_09030"
     CDS             <1..777
                     /locus_tag="VF226_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7074169.1"
                     /translation="IASGGEFVATNVLGTHTLLEAATRHRVDRFLHVSTDEVYGTLGP
                     TDPAFTETTPYSPNSPYAASKASSDHLVRAYHHTYGLPTLTSNCSNNYGPFQFPEKLI
                     PLVIRNALAGEPLPVYGDGQQVRDWLYVLDHCAAIRRILDQGRIGQTYNIGGRSEKKN
                     IDVVRTLCAFLDEAKPRANGRYAEQITYVKDRPGHDRRYAIDDRKLARELGWQPQESF
                     ESGLRKTVQWYLDHQEWVSEVTSGAYRTWVETNYAFRGAA"
     gene            774..1652
                     /gene="rfbA"
                     /locus_tag="VF226_09035"
     CDS             774..1652
                     /gene="rfbA"
                     /locus_tag="VF226_09035"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX7074170.1"
                     /translation="MKGIILAGGSGTRLYPVTQVVSKQLLPVYDKPMVYYPLSTLMLA
                     GIRDILVISTPQDTPRFQELLGDGTRWGLRLSYAVQPRPEGLAQAFIIGREFIGADRV
                     ALVLGDNIFHGHELSSLLARGVSREAGATVFAYPVNDPERYGVIEFDAQGHAISIEEK
                     PKQPKSRYAVVGLYFYDNRVVDIASRLRPSARGELEITDVNRAYLERGELDVIPMGRG
                     MAWLDTGTHETLLEAAHFIETIERRQGLKIACPEEIAYRLNYIDAHQFAALAKLLANS
                     RYGEYLTRVLSDRIFG"
     gene            1679..>2090
                     /gene="rfbC"
                     /locus_tag="VF226_09040"
     CDS             1679..>2090
                     /gene="rfbC"
                     /locus_tag="VF226_09040"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX7074171.1"
                     /translation="MLVLEPRVFQDERGLFFESYNKRSFRKATGIAPDFVQDNVSRSA
                     KNVIRGLHYQVGRPQGKLIRVVSGRIFDVAVDLRRSSASFRRWIGIELSQVQLVWIPP
                     GFAHGFLVLSDSAEVHYKVTEFWDADSERGLAWND"
BASE COUNT          383 a          696 c          649 g          362 t
ORIGIN      
        1 atcgcgtcgg gcggggagtt cgtcgccacc aacgtgcttg gcacgcacac gctgctcgag
       61 gcggcgaccc ggcaccgggt cgaccgcttc ctgcacgtgt cgaccgacga ggtctacggc
      121 accctcggtc cgaccgaccc ggcctttacc gagaccacgc cgtattcgcc caacagcccg
      181 tacgccgcct ccaaggcgtc gtccgaccac ctggtgcgcg cctatcacca cacctacggc
      241 ctgcccacgc tgacgagcaa ctgctccaac aattacgggc cgttccagtt cccggaaaag
      301 ctgattccgc tcgtcatccg caacgcgctc gccggcgagc cgctgcccgt gtacggcgac
      361 ggccagcagg tgcgcgactg gctctacgtg ctcgaccact gcgccgccat ccgtcgcatc
      421 ctcgaccaag ggcgcatcgg ccagacctac aacatcggcg gccgcagcga gaagaagaac
      481 atcgacgtcg tgcgcactct ctgcgccttc ctggacgagg cgaagccgcg cgcgaatggc
      541 cgctatgccg agcagatcac atacgtgaaa gaccgcccgg gccacgaccg gcgctacgcc
      601 atcgacgacc gcaagctcgc ccgcgagctc ggctggcagc ctcaggagag cttcgagagc
      661 ggtctgcgta agacggtgca gtggtacctc gatcaccagg aatgggtgag cgaggtgacg
      721 agcggtgcct atcgcacctg ggtcgagacc aactacgcgt tccggggcgc ggcttgaagg
      781 gaatcattct tgccggcggc tcgggcacgc gcctctaccc cgtgacgcag gtcgtctcca
      841 agcagcttct gccggtgtac gacaagccga tggtgtacta cccgctgtcg actctgatgc
      901 ttgccggcat tcgcgacatc ctggtgattt cgacgccgca ggacacgccg cgcttccagg
      961 agctcctcgg cgacggcacg cggtgggggc tgcgcctttc ctacgccgtg cagcccaggc
     1021 ccgaaggctt ggcgcaggcc ttcatcatcg ggcgcgagtt catcggcgcg gaccgcgtcg
     1081 cgctcgtgct cggcgacaac atcttccacg ggcatgagct ctcgtcgctg ctcgcccgcg
     1141 gcgtcagcag ggaagcgggg gccacggtat ttgcctaccc ggtcaacgat ccggaacgct
     1201 acggcgtcat cgaattcgat gcccagggcc atgccatcag catcgaggaa aaacccaagc
     1261 agccgaagtc gcgctatgcg gtcgtcgggc tctatttcta cgacaaccgg gtggtcgaca
     1321 tcgcctccag gctgcggcca tcggcccgcg gcgagctcga gatcaccgac gtgaaccgcg
     1381 cctacctgga gcgcggcgag ctcgatgtca tccccatggg gcggggaatg gcgtggctgg
     1441 ataccggcac gcatgaaacg ctcctcgaag cggcgcattt catcgagaca attgagcgcc
     1501 ggcaaggcct caagatcgcc tgtcccgagg agattgccta ccgcttgaac tacatcgacg
     1561 cgcaccagtt cgcagcgctc gccaagcttc tcgcgaacag ccgatacggt gaatatttga
     1621 cgagagtcct ctcggaccgc atctttggat gaaagttatc cgtacggata tccccgaggt
     1681 gctggtgctg gagccacggg tgttccagga tgagcgcggt cttttcttcg agagctacaa
     1741 caaacgatcc ttcaggaagg caaccggcat agcgccggac ttcgtgcagg ataacgtttc
     1801 acgatcggcc aagaatgtga ttcgcggcct gcactatcag gtgggcaggc cgcaaggcaa
     1861 gcttatccgc gtcgtcagcg gcagaatctt cgatgtggcc gtcgacctgc ggcgttcgtc
     1921 cgcgagcttc cgccgctgga ttggcatcga gctgtcgcaa gtgcagctcg tctggattcc
     1981 cccggggttt gcccatggat tcctggtgct ctcggatagc gcggaagtgc attacaaggt
     2041 gacggagttc tgggacgccg acagtgagcg cggcctcgcc tggaacgacc
//
LOCUS       DASVDU010000144        51957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_978506, whole genome shotgun sequence.
ACCESSION   DASVDU010000144 DASVDU010000000
VERSION     DASVDU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 51957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51957
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978506"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2676)
                     /locus_tag="VF226_09045"
     CDS             complement(160..2676)
                     /locus_tag="VF226_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX7074172.1"
                     /translation="MKLDRKPLVITAAALILLPFVLSAIGLGITSASEVVIFAIACMA
                     LNILVGHTGLVSFGHGAWFGLAAYAAGLAQRNLFPDAFILPILLGLAVIVAVSAGFGF
                     LILRRRGVYFALLTLALSAMLYAVAFRWTSVTGGEDGLGGIRRPQAFGIDFENATNYY
                     VLMALIGFGVLYALWRFHRSPVGHVLVAIRENEQRARFIGYPTDRYKLLAFVASATLT
                     GLAGMLLLFNNRMTSAEPISIAFSGELLAMVVIGGMRSFLGPALGALFYVIFRDFLSS
                     ITPNWLLYFGLMFVGFVVFSPEGLVGVYERLTAPFRPKLVEDAAMAGRQAGEIDLPNF
                     LKPATHTAGTILVVNAIAKSFGGIKAVQGASFALRDRTLHALIGPNGAGKTTAFNVIS
                     GLFPPDAGQIKLADRDVSRLSPEALTQAGIGRSFQITNLFPALSVEENVRLAVQARSP
                     HRFDVWTAALDLADVNAETAEILRTMGLKGVERAEAGSLSYGGQRLLDMSLALATKPR
                     VLLLDEPLAGLAAAERERVGNLIKRISADIPVLLVEHDIDRVFQIADAVTVMNEGQVL
                     VDGSVDDARNSQRVQEVYIGSGAHALSEKHRATSAKDEPLLTLEGVDTFYGKSHVLRS
                     VSFSVRENEIVALLGRNGAGKSTLLKSIVGITPPRRGSIRLGGEELAGQPSAVITRRG
                     ISYVPQGRGLFAGMTVAENMELGRLKRRNGAGTFWDEEKIFTFFPRIKDRWRTPADYL
                     SGGEQQMVAVARALIGDTRVLLLDEPFEGLAPAVIEELFESFDSLRHQISIVIVDHHL
                     DLALALSDRTVALERGRVIHTGPSQALSEDFELRRKVLWL"
     gene            complement(2686..3564)
                     /locus_tag="VF226_09050"
     CDS             complement(2686..3564)
                     /locus_tag="VF226_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7074173.1"
                     /translation="MSATLFAQQVLNGLLDGVYYLLIALGLSLIFSLGGIINLAHGAF
                     YAIGAYLTVVIAPHLGFSGALVVSPIAVALLGILIERFLFRRFYRSDPILSLLLTFGL
                     AMVAEQSLRMIFGAPPMSFGLPQALRGPIIVGDFIYSYYRTLLLGVAAVCVIGLWLLL
                     NKTAFGRVVRAGVQNPDMVGALGISLQPYMAAVAALGIGLAALAGVLLAPIYSIHPAM
                     GQEIITPAFVVVVIGGLGSFWGVIVAALLVGLVKGMTIAFGYAQASTAVIYFMMLLVL
                     LFRPRGLFGERIQKFE"
     gene            complement(3637..4890)
                     /locus_tag="VF226_09055"
     CDS             complement(3637..4890)
                     /locus_tag="VF226_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074174.1"
                     /translation="MSIKRKLSRRSVLKGAAGAAAAGVLGFPAISKAQSDVIKIGHLT
                     PRTGFLGTLGEFAVQAADLAVEEINGKGGVLGRKIELLKEDSVNPQTASTKAERMIER
                     DQVACIVGEISSASALTIAQVAQRTKNLFVNTGANSDALRGSNCNKYMFHVESQNSMY
                     VKTVGRALLAQNMVKGKKWYSLTADYAFGHDLLRVAKRFMEANGGEFAADELVPTDAA
                     DFSAFLVKIRDAKPDLVVSNLAGVQITNFLKQFSEFGLDFPVAGFGFDTAVAWGAGQG
                     NFFGTWPLVWHHLVDTPGSKAFVAAFTKKYGRPPDNQAWGDYVAMHILAKSMNEIKSI
                     EAPKLIEHWEKGAKFDLLKTREGYFRAYDHQLMHEMYAVEALKASEIKNQWDIYKPGP
                     PTPGPDEDLEVIAATKEENVCNMSA"
     gene            complement(5057..5932)
                     /locus_tag="VF226_09060"
     CDS             complement(5057..5932)
                     /locus_tag="VF226_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074175.1"
                     /translation="MADERPSTERKDGIGWYLSFALIAVLAFIAIGTITGQEALAAAA
                     RVFWNGFAFIINGLFRLFGGLLGLIAKGVGWRRATRIANVIGGIGLGYAASVVLSEDA
                     VQKARGWRGKLRATINSVKTRWQNLPLLWKLVIVAALIASQVYLHTVLVIFPIAFLVP
                     VVRKLWIRTADLIFGVWYWKTFGTWHRRLVAWLKKLPGVRAVIGWVRLTRIRYLTAWR
                     LWKYHPCYFDCETGRRQVSFVEPLRLWWGGELDGYIGRPLLAGPMHRPSPPSLEARSL
                     GLEVADERHQLPRTG"
     gene            complement(6086..6841)
                     /locus_tag="VF226_09065"
     CDS             complement(6086..6841)
                     /locus_tag="VF226_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEX7074176.1"
                     /translation="MNATSPEAARKAGAAVMPAARRRGFETTSTWIFDLDNTLYPAEC
                     NLFAQVDRRMGEYIAQYLGVPFEYARHLQKSYYRQYGTTLCGLMAVHKLDPKPFLDYV
                     HDIDLSAVPELPELAAAIEKLPGRRLIFTNGSRRHAERVAGKLGVLHLFEDICDIAAC
                     EYVPKPSPDAFDRMARRHGVEPQTAAMFEDMPQNLEAPHALGMTTVLVHSSYYDHPIQ
                     LKIREWREPPEHVHHMTENLVGFLEEIASLPKA"
     gene            complement(6838..7782)
                     /gene="argB"
                     /locus_tag="VF226_09070"
     CDS             complement(6838..7782)
                     /gene="argB"
                     /locus_tag="VF226_09070"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7074177.1"
                     /translation="MAGRTDKVQVPGAHGAVSAHSKAQILAEALPYLQRYDNQTVVIK
                     FGGHAMGDAALADAFAQDIVYLKLSGVNPIVVHGGGPQIAAMLKKLEIKSEFINGLRV
                     TDKPTVEVVEMVLAGAINKEIVSAINRQGGKAVGICGKDANLMIARRITEMPDPESNI
                     MKAVDIGYVGDPEEVNPHIVEVISNSDLIPVIAPVGVSRDGQTLNINADTFASALAAK
                     MKAKRLLLLTDVEGVLDKDKKLIPELSVAKARELIRNGTITGGMIPKIEGCIEVVEAG
                     VEAVVIIDGRVPHCVLLELFTEHGVGTLVGTSEEPALS"
     gene            8034..10049
                     /locus_tag="VF226_09075"
     CDS             8034..10049
                     /locus_tag="VF226_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7074178.1"
                     /translation="MSQSYAEALSWVPKSRGLATTLARAFDYAASQSHKSVLLEHLLL
                     ALISDADASAVLQACRVDLNRLDSDVTNYLAGVDERLSPGDTSAPVASPELLRIVEYA
                     SAAARQSRRREVNGAIVLAAIVGDGRSPAASMLREQGLTFEEAIKALQKANAAAREPG
                     TRPASVSAPRPRPAAPPPQAAPAAPSYPDEQAAADPHLSPQQPGPRLSHPEQHPDRDR
                     PAAASAVNGVAGGQTTEEILAAARRRVGSGLTGGQPGAKPETQDAPRQQPERHPADDV
                     AHQEQPEDERDDAIASQEHSAGPPPGEDRHLVPPQPQPPGRPQGPMRPHAAPQPADYS
                     RSYPPPGFGQNAPAEDLGTQAPQPRRSDAPSRTTPPPRGAPAPEYGPTLDWPRPAPSP
                     QDSYYRPHRPDTDDTEQVRRQPTAHPQQERFHPPPPPPPHFPGHPAAGPSRPGRAPWP
                     EPPAPAYPQQGTYGSPQPAAARAQPELRGRAPNADEAPATQVFAGQLVENIPRTMTVG
                     VPELVEVSIAKAELAGLAEKLQGSGAAYRHDIVVTKAMSVRLRAPEGGFWIETASPET
                     QWIENSLGLLSDDYASWRWTVTPQRRGRARLQLIVSARTVGADGLAAETALPDQVIEV
                     KIRANYGRTAVQWGGWIAAAVLGGIFAKFGEGLLSFGGTVLSLLGIL"
     gene            complement(10066..10707)
                     /gene="yihA"
                     /locus_tag="VF226_09080"
     CDS             complement(10066..10707)
                     /gene="yihA"
                     /locus_tag="VF226_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX7074179.1"
                     /translation="MTQEWLATGAALFGRPWGFVRGVPSLEFLPDADRPEIAFAGRSN
                     VGKSSLINALVGQHGLARTSNTPGRTQELNFFATADVPLYLVDMPGYGFARAPKEKVK
                     AWIGLVRDYLRGRPTLKRVFLLIDSRHGLKPPDRETMRLLDEAAVSYQVVLTKADKPS
                     RSELEKVVAATKLALTAHPAAYPEVIVTSAAKGVGIDVLRARIAELCLTQSSG"
     gene            complement(10801..12765)
                     /gene="yidC"
                     /locus_tag="VF226_09085"
     CDS             complement(10801..12765)
                     /gene="yidC"
                     /locus_tag="VF226_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX7074180.1"
                     /translation="MEQESQKNLLIAIILSIAVLLGWQMIFGTPQAPRNEAGRPAPTE
                     TAPEAAKPAAPGTVAPPSAPAVAPTRQAALAESPRVEIDTPSVRGSIALKGGRIDDLV
                     LKRYRETVDPNSPNIVLLSPPGGPDSYFAEYGWVAPSGTNLSLPGPDTVWQVEKGGPL
                     TPNNPITLVWDNQQGLIFRRTISIDDKYMFKVTDAVENNTGGPVTLYPYARLRREGTP
                     HVQGFYILHEGLIGVLGEAGLHEITYANALKANGGATVTDAKGGWLGITDKYWAATLI
                     PNQSVSYNASMTGQAGTAGQPDSYQADYLLPAVTLPAGGKSETEGMFFAGAREVGTVQ
                     DYATTYDIKQFDLLIDWGWFYFITKPLYHLLNWLYGLLGNFGLAILAVTVIVKIAFFP
                     LANKSYESMAKMKKLQPEMERIRDRFKDDKMRQQQELMALYKKEKINPLAGCLPIAIQ
                     IPVFFALYKVLFVSIDMRHAPFFGWIKDLSAPDPTSIFNLFGLLPFAVPDFLHIGAWP
                     IIMGITMWLQMQLNPQQPDPIQQKIFNLMPVVFTFLLAAFPAGLVIYWAWNNILSLGQ
                     QWLIMKRQGVEVPILANLKRNFAPVVGLVSGTKAVEKKTPAQSAPKAKEKASETEKTE
                     ADAAADGSESPTEGAAEAPKAEPKKRKAKR"
     gene            complement(12879..13256)
                     /gene="rnpA"
                     /locus_tag="VF226_09090"
     CDS             complement(12879..13256)
                     /gene="rnpA"
                     /locus_tag="VF226_09090"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX7074181.1"
                     /translation="MGLTTLKRRSEFLRVRGGLRWATPSLVLEAKPRAAISPEGGSSE
                     GERPRFGFTVSKKVGKAVVRNRVRRRLKAAVRELLSSARAGFDYVIVARPVAAERAYA
                     DLKADLAEALARIHRSGRSKRVR"
     gene            complement(13317..13451)
                     /gene="rpmH"
                     /locus_tag="VF226_09095"
     CDS             complement(13317..13451)
                     /gene="rpmH"
                     /locus_tag="VF226_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX7074182.1"
                     /translation="MKRTYQPSRLVRKRRHGFRKRMMTPGGAKVIARRRAKGRKRLSA
                     "
     gene            13816..14577
                     /locus_tag="VF226_09100"
     CDS             13816..14577
                     /locus_tag="VF226_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7074183.1"
                     /translation="MNPRRWLPLVLLLTATGLAFGMGWHRLLTFKAVGLNYEALSDFI
                     GAHLVVALLLYVGAYVAVVALSLPGGLVMTVAGGLLFGWELAAPVTVLSATIGASIIF
                     LVAKTSLGEPLAAKAGPWLGRLRAGFQENALSYLLFLRLVPLFPFFVVNLAPALLGVP
                     FTTFAIATLLGIIPGTLAFSVAGSGLASVIEAQNRIYQDCLARAPASQEANCPYTVDV
                     GALVTPELIAAFILLGLMALVPVAVKKWSKRNAEA"
     gene            14564..16045
                     /locus_tag="VF226_09105"
     CDS             14564..16045
                     /locus_tag="VF226_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074184.1"
                     /translation="MLKRDVGDGGAGKILPSASKSDVITADLCVIGAGPGGLSVAAAA
                     AALGRSVVLVEKHKMGGDCLNYGCVPSKALLAAAKRAHIMRTSDKFGIASVRPEVDWQ
                     AVRDHIRDVIAEIAPNDSVERFTALGVRVIQAAGRFVNKRTLQAGEHLIRARRFVIAT
                     GSSPVLPEIPGIDDVPYFTNETIFENASPIAHLIVIGAGPIGLELAQAYVRLGSRVTV
                     LESGTALGKEDPELARYLVNELIREGIDVREKVAIERIDGCEGEVRVSIKTGNASESI
                     AGTHLFVATGRRPNITDLNLEAAKVKYDSRSGIKVSQGLVASNRRIFAIGDCAGGPQF
                     THVANYHAGITIRRALFRLPAKVDERLMPRVTFTEPELAYVGLSEEQARLAGEIRVLR
                     WPYSENDRVQAERATRGFVKVITDKRGRVLGAGIVGQQAGELIEIWSLAISQNMKIKA
                     LADLVLPYPTLSEVNKRAAFRYYATAASSPVVRKVVSMLAKLG"
     gene            16067..17608
                     /locus_tag="VF226_09110"
     CDS             16067..17608
                     /locus_tag="VF226_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7074185.1"
                     /translation="MPQDSAPPGKNGGRANEAVEAVSDAPTHSAWEIRLHGLPSKLLY
                     LTALFVMLAEVLIFVPSVANFRVTWLTDRLTAARLAALAADAAPGGIIPENVRSELLV
                     TAQLRSVAVKAGGMRKLVLPPDGNFVVDAQFDLREPPESTVLERLSNRFALIADAVGV
                     FFAPAGRMILVRGHPDTGPRPRLPMTDFIEIVLPEATLKAAMVRYGLNVLGLSIIISI
                     IAAALVYVALSAMLVRPMMRITRNMLAFSENPEDPSRIIVPTDRRDEIGIAERELARM
                     QRQLAQTLHQKSRLAQLGLAVSKINHDLRNMLASAQLISDRLTALPDPTVQRFAPKLI
                     ASLDRAISFCNDTLRFGRAEEAAPRRELLLLRNLVEDVGESLGLPRDGIDWHVAIDET
                     LRVDADPEHMFRVLNNICRNAVQAIESQGPQAAGEVVVEARREGLRSIITVRDTGPGL
                     SAEARARLFQAFGSSARNGGSGLGLAIAAELVRAHGGTIEVLDVPKGAVFRIEIPDRG
                     TGTRN"
     gene            18052..18369
                     /locus_tag="VF226_09115"
     CDS             18052..18369
                     /locus_tag="VF226_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX7074186.1"
                     /translation="MKESQLQKMSYKELLQLQKKVADAIAKKRGSERAALKQKLQSLA
                     EQAGFQIDDLFGGPRRERAASSSITVKYRHPTNRSLIWTGRGRRPKWLVAEGGDVERF
                     RVA"
     gene            18412..18792
                     /locus_tag="VF226_09120"
     CDS             18412..18792
                     /locus_tag="VF226_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7074187.1"
                     /translation="MKAPAAKTKAKKAAAPAKAAEKAPAKAAAKAPAKAATITLNQMA
                     KELSESHEMPRKQAAALLTDLTSMIQKHLKKGARVRIGGLGVLQVRKRPARMGRNPAT
                     GEPIKIKASKKIAFRPAKELKELI"
     gene            complement(18870..19418)
                     /locus_tag="VF226_09125"
     CDS             complement(18870..19418)
                     /locus_tag="VF226_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074188.1"
                     /translation="MPNWKCTAVGLALTGIAFATPAAAGGYPEDAGYYAYVSEPELIE
                     APGGEKIIQTFTEVRIRYNGAPRYGYGAAFVNGPPDLWRQREVAYDAIFTRDKRVIYD
                     SNRAAQTRAAVGFHGPVVYSGPRPYHPYGSVKDYGPPPVGYFAPPFNPAGTCGTFRYW
                     NGETCVDARFYSRYKNPYKWYR"
     gene            complement(19697..21355)
                     /locus_tag="VF226_09130"
     CDS             complement(19697..21355)
                     /locus_tag="VF226_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7074189.1"
                     /translation="MTEKMAGDIELQIMWNRLISVVEEQAQTLLRTAFSAIVREAGDL
                     SAGIFDRQGRMLAQAVTGTPGHVNSMAESVKHFIAHFGLENMKPGDAYITNDPWMGTG
                     HLNDFVVTTPCFHKGKLVGLFSCTSHLMDIGGIGFGPDATDVFMEGLYIPFLKLLDEG
                     KVNETLMAMIRANTRLPIDTVGDVYSLAACNDVGCQRLIEMMEEFELDDLDQLAEHII
                     ERSREAVLAEIAKLPKGTWSNTMVTDGYDEPIVLSATTTISDTGIHVDYTGSSAPTRR
                     GINVPLAYTTAYTAFGLGCIVASKVPNNAGSLAPLTVSAPADTILNAPKPSPVLIRHV
                     IGQMLPDVVFGCLRQAIPDRVPAEGAACLWNLNVRGHVKGGEGGNYGFMMTVTSNGGT
                     GARPQSDGLSATAYPSGVKGTPIEIAEQITPLLFWKKELRPDSGGAGRTRGGLGQIIE
                     IESNIGEPFELLAGFDRIDYPARGCDGGHNGANGAVALASGKVLKGKGFQLVPPGERL
                     VIKTPGGGGMGPPSERDRKATLRDLEDGRISAEAARSIYGVDPA"
     gene            complement(21352..23451)
                     /locus_tag="VF226_09135"
     CDS             complement(21352..23451)
                     /locus_tag="VF226_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7074190.1"
                     /translation="MGSVTPELRLAVDIGGTFTDIVLDNGSERLTSKVLTTPSAPERA
                     VVEGTAAILASAGKTFADIRLFVHGTTLATNAVIERKGARTALIATEGFRDVLEIADE
                     SRFDQYDVFIQKPQPLVPRRLRFTVPERLDVHGTVKLPLDEAAVVRVANRLKAEEIEA
                     VAVALIHSYVNPMHEERIRAILHEHCPGLSITLSSEVCPEAREYERTSTAVANAYVQP
                     LMAGYLGRLEQEFRSRGCTAPIHLMTSGGSLASLKTAVNFPVRLIESGPAGGAILAAR
                     IAAERGEDRILSFDMGGTTAKICLIDEGRPFKARVFEVDRSSRFMKGSGFPLRIPVIE
                     MVEIGAGGGSIARVDALKRINVGPDSAGSEPGPACYNRGGTEPTVTDADVALGKIDPA
                     RFAGGKIEIDARKAAAALDTHIGQPLELETDAAAYGVAEIVDENMANAARVHAVERGA
                     EVRARTLVAFGGAAPLHAARVAEKLNIQRVIIPAGAGVGSAIGFLDAPAAFELVRSLY
                     MRLDRFDVAAANALLESMSAEATALAREAAGDAPLSEQRRVFMRYSGQGHEIPVELPS
                     RSLTETDPASLRQAFEAEYDRLFARHIPGAAIEIMSWVVLVSGAESRPARLGPPVPQP
                     PPQPTGTRVVFDARLGKRIEVPVFERGNLQPGTQISGPAVIVEDATSSYLSTTFDAIV
                     DAGMALVLTAKTSEARS"
     gene            23923..25812
                     /locus_tag="VF226_09140"
     CDS             23923..25812
                     /locus_tag="VF226_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7074191.1"
                     /translation="MAQRKGSAATARTPASDTLEPVGALTPVHKADIQRESIPRKPSP
                     PPPPSVYETDTATIFGEAMDKSLNYLVSRFTMGLSPVALTEAYLDWLIHLGAAPGKRM
                     ELGERAIRYMARLARYTATCAMSHQAVQPCIEPMPNDNRFRREEWQLWPFNVIHQAFL
                     LNQQWWHDATTGVRGVTKQHERELEFASRQLLDIFSPSNFLWTNPTVLQETASEGGLN
                     LVRGYLNFIDDWQRTLNHRRPPGMEEFKVGETLAVTPGKVVYRNRLIELIQYEPATDQ
                     VRPEPILIVPAWIMKYYILDLRPGKSLVEFLTKQGFTVFMISWKNPTREDRDLRLEDY
                     RRLGVLASIEAVQKIVPGQDIHGVGYCLGGTLLAIAAAALARTRRDPFKTLTFFAAQV
                     DFEEAGELMLFINESQLVFLDDMMEMQGFLGNYQMAGAFQLLRSNDLIWSRIINEYLM
                     GEREPMFDLMAWNADTTRMPYFMHSEYLRRLYLNNELATGAFVVDDRPVALTDIRKPV
                     FAVGTETDHVAPWRSVYKMHLLLDTELTFLLTNGGHNAGVVSEPGHPRRAYRVHTRKE
                     DDLYVDPDTWFNETPVKRGSWWPEWAAWLAERSGDWTSPPRMGAPESGFRPLVDAPGS
                     YVLQL"
     gene            25832..26815
                     /locus_tag="VF226_09145"
     CDS             25832..26815
                     /locus_tag="VF226_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX7074192.1"
                     /translation="MRAMVLEQPRTELKLLDLPVPEPGPGEIVVRVAACGVCRTDLHV
                     VDGELPDPKLPIIPGHEIVGYVTKLGPGVTAFETGDRVGIPWLGRTCGTCRYCLAGRE
                     NLCERPGFTGYTIDGGYAEYAAARADYCFPLPSEFEDASAAPLLCAGLIGYRSLKLGG
                     PGRELGIFGFGAAAHIVAQVARYEGRRVYAFTRPGDAAAQEFARSLGAEWAGGSDETP
                     PCPLDVAIIFAPVGALVPAALKGMARGGTVVCGGIHMSDIPSFPYELLWHERVVRSVA
                     NLEREDAREFLALAPKVPVETTIETFPLEAANDALARLREGRLTGAAVLIP"
     gene            26919..27212
                     /locus_tag="VF226_09150"
     CDS             26919..27212
                     /locus_tag="VF226_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074193.1"
                     /translation="MLTIEDMVAFSGLSRDEILAIAEHEHLPEAAACALAEYLMQHKH
                     GCERIREMIVEDIRAAQERGDREHVKTLLHVLHHFVRNHPESLPIGRGETDVT"
     gene            complement(27517..27717)
                     /locus_tag="VF226_09155"
     CDS             complement(27517..27717)
                     /locus_tag="VF226_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074194.1"
                     /translation="MTPVPCQITPDMIVDYAAGRLDDRDADVIETAMSQNGAVAAAVT
                     RARQLNSRIGRYFSRRDHQNRR"
     gene            28044..28337
                     /locus_tag="VF226_09160"
     CDS             28044..28337
                     /locus_tag="VF226_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074195.1"
                     /translation="MLRVFLATTNIFTSLVLGALAMGAVWYFSPDLMQSFFQSASGLK
                     SWLTNLGIEPKYNNFLWFLIEERQLVFMGFVIATRVAMAILAAIFIAPFTEAV"
     gene            complement(28350..29102)
                     /locus_tag="VF226_09165"
     CDS             complement(28350..29102)
                     /locus_tag="VF226_09165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX7074196.1"
                     /translation="MRKAARSTGKRAAKELHLQGIPATVEVRHHPAARRMTLRVSRTR
                     RTVIVTLPMQCDLEQAGQFVSRHIDWVKARLGSLPTPVPFAHGAEIPLRGIPHTIHFI
                     GRLQRGPVVSIAENEENRRELVVSGDPAFAPRRLQDWLIAQARSDLDDRVAWHSRNLN
                     LRPRGIAVRDQVSRWGSCSTTGMLSFSWRLVLAPPLILDYVAAHEVAHLAEMNHGPRF
                     WALVRKTMPRMDEAKQWLRVYGLDLHRFGSED"
     gene            complement(29247..30791)
                     /gene="phaZ"
                     /locus_tag="VF226_09170"
     CDS             complement(29247..30791)
                     /gene="phaZ"
                     /locus_tag="VF226_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX7074197.1"
                     /translation="MLYHWYELTHVAVRPARVAADAGRLFFNNPLNPLTHTGWGRHAA
                     AACEVFERTTRRYHKPDFRISSTTVDGQPVAVTENVVWQRPFCRLVHFARDLPLERRV
                     QDPRVLLVAPMSGHFATLLRGTVQTLLPNHEVYITDWQDARDVPLVAGRFDLDDYIDY
                     MRDMFHFFGGDVHVFAVCQPSVPVLAAVALMEEDDDPSVPLSMILAGGPIDTRVSPTV
                     VNKLAVERGTDWFRRNVITTVPWPRLGFGREVYPGFLQLTGFMTMNLDRHLKAHKDLF
                     NHLVRGDGESAEKNREFYDEYLAVMDLTAEFYLQTVDSVFVRHMLPKGEMVHRGRRID
                     PAAIRRVALMTIEGEKDDITGVGQCAAAHDLCVNIPGDSKEHFVCPKVGHYGIFNGSR
                     FRSEIAPRIAKFIRTHDQRSRTLDELAYANASAAAERGGYQNELASIAFTFSPANDCT
                     PDPMVSKLKAQGLGSIAPRYDTTNSASGMFAAPYRIMALAGGLFLENLLRWNFPLSSG
                     EPKERS"
     gene            complement(30937..31545)
                     /locus_tag="VF226_09175"
     CDS             complement(30937..31545)
                     /locus_tag="VF226_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7074198.1"
                     /translation="MRLRLLALIAAGFVGGAAVGLAVVPGALDSLQAPSQVTSIGKAA
                     VGGPFTLTDHTGRRVTDKDFRGKYMLVYFGFTFCPDVCPSGLQVISAALDELGPKADK
                     ITPIFITVDPERDTPEQLAQYVSSFHPRLIGLTGTPEEIQAVAKEYRVYYRKVKDEKS
                     TAEYTVDHTSIIYLMNPQGEFVTHFTHATPVATMVEKLKKVL"
     gene            complement(31596..32423)
                     /locus_tag="VF226_09180"
     CDS             complement(31596..32423)
                     /locus_tag="VF226_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074199.1"
                     /translation="MTTKLLPQTGLSASARRIRGMVRRHWYLIRSSGPRTAELIFWPL
                     VSMLMWGFLQTHLAKTTSLAAQAAGLFVGGVLLWDILVRSQLGFSVAFLEEIWSRNLG
                     HLMMSPLRPIELIASLMLVSLMKLTVAMVPVAILAYIFFGFNLLGLGIAFAAFFANLI
                     IMSWALGLVSTGAVLRWGLGAESIAWLIVFILLPISCVYYPVSTLPEWLQPLALALPP
                     TYVFEGLRGLVLQQTFHGDYMLRALLLNIVYVSGALILFRALLESARRNGALVQLGE"
     gene            complement(32435..33232)
                     /locus_tag="VF226_09185"
     CDS             complement(32435..33232)
                     /locus_tag="VF226_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074200.1"
                     /translation="MLQSGARTDGAGTVLPVQVRDLRKVYGEVVAVDNVSFSLSAGSI
                     TALLGGNGAGKTTTISMLMGLVVPTSGDVRVFGVDMVRNRHAALHRLNFESPYVEIPM
                     RLTVRQNLEIFGQLYGVADVAGRVREIADELRIGELLDRLYGKLSAGQKTRVSLAKSL
                     LNEPELLLLDEPTASLDPDTADWVRSVIADYCRKRSATIVLASHNMAEVERLADRVIM
                     LERGRILADETPAELIRRFGRETLEEVFLDIARRRAPDTFELLNGRE"
     gene            33368..34741
                     /locus_tag="VF226_09190"
     CDS             33368..34741
                     /locus_tag="VF226_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEX7074201.1"
                     /translation="MAVTRWMGLEERLEAEQVESRLRLLTIVRLRWFAVVGQLLAVSV
                     VHFGLGFEFPFGICLLLIAASAWLNVFLSVRYPARHRLGSGFATVLLTYDILQLVALL
                     YLTGGIENPFAVLIVGPVTVSAATLPPINTVFLGGLALASTAFLIIDHWPVPWYATEL
                     LTLPMVYKVGQLAAVAATMIFLALYVWRLSREARQMSAALTATELVLAREQKLHALDG
                     LAAAAAHELGTPLATIVLVTSELQRDIEPGTPLAEDLALLRSQADRCREILQKLTRQP
                     SEQDPLHATLSVHQLLDEAAEPYRNSGKAIEISCAPAEGVSGPPAREPVGERRPGLIY
                     GVGNLIENAADFARSKVEISARWNEDTLEIEVKDDGPGFPPELLETLGEPYITTRPAG
                     SGGPGKRAAGLGLGFFIAKTLLERSGATLTLDNRAPPEHGAIVRISWAREVFEAFSPG
                     PPGAAVS"
     gene            34842..35405
                     /locus_tag="VF226_09195"
     CDS             34842..35405
                     /locus_tag="VF226_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEX7074202.1"
                     /translation="MAEEFAFSKEELPEDTSLVIVDDDRAFLQRLGRAMENRGFEVRT
                     AQSVAEGLELIRQKAPAFAVVDMRLEDGNGLDVIAELARIRPNARAIVLTGYGNIATA
                     VSAVKLGAVDYLAKPADADDVTDALLAPPNAKAAPPENPMSADRVRWEHIQRVYELCN
                     RNVSETARRLNMHRRTLQRILAKRAPK"
     gene            complement(35441..35938)
                     /locus_tag="VF226_09200"
     CDS             complement(35441..35938)
                     /locus_tag="VF226_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX7074203.1"
                     /translation="MAIPIGLDKGPLNDGRQSATALQIARGVGRLLASLGLASISELV
                     LPNGRRADIVGLSEKGEFWIVEIKSSVEDFRSDQKWPEYWEYCDKLYFAVSPSFPQEL
                     IPETAGLIVADRYGGEIVRSVDEVRLPAHRRRVMSLRFGRVAAARLQSIADPGSALGN
                     LLRGA"
     gene            36079..38598
                     /locus_tag="VF226_09205"
     CDS             36079..38598
                     /locus_tag="VF226_09205"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7074204.1"
                     /translation="MFFKRAPQGNKSAAAEAQGSAGQVPSMEAAPHPPLPVSELRRNV
                     DPASLGFGTTAELEPITGLIGQDRALRAIEFGANMRSYDFNLFVLGPPASGKSTAVRD
                     YLTKKAAEMPPPPDWVYVNNFENQNRPRALQLPAGRGRKLAEQMVAAIDELRNTIPAV
                     FESEEYQARRRAIEEQFRSSQEEAFEELNRKAQAQNIAILRTPMGFAMAPVHEGKVVK
                     PEVFNALPEDMRKNVQAKIEALQKELEAILERVPKSDKERRNKLSELIEEMAQGAVRE
                     ALDDVRANFSDLPKVLEYLDAAGKDLIRHVGLFVAQPGDENELIKQAIDTSRDPRFRR
                     YMVNVIVGNGESFPGAPAIEEINPTYGNLIGRIEHIAQMGALVTDFLLIKPGTLHKAN
                     GGYLLIDARKLLLSPYAWEALKRTIKAREIRIEQPLESLGLITTQTLDPEPIPLDVKV
                     VLFGDRELYYLLAAFDPDFRGLFKVQADFDDTISWSDENNLAYARVIASIVEKYKLKP
                     VDASGVARVIEEGARLADDREKLSIEIGRISDLIREADYWSSMAAREVTTREDVERAI
                     KEGIERASRVRDRAQEIIERDIVFIDTDGAKVGQINGLSVIQIGTFSFGRPSRITARV
                     RMGQGRVVDIEREVKLGGPLHSKGVMILWGYLAGRYAEDVPLALAATLVFEQSYGGVD
                     GDSASSTELYALLSALSEVPVKQGFAVTGSVNQKGEVQAIGGVNEKIEGFFDICKARG
                     LTGNQGVLIPKANVQHLMLREDVVEAVREGKFAIHAVSHVDEGIEILTGIKAGERGED
                     GQFPPGSVNRLVEDKLKAFAERAKAFGRSRGDSQDNGKNSA"
     gene            38595..39458
                     /locus_tag="VF226_09210"
     CDS             38595..39458
                     /locus_tag="VF226_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074205.1"
                     /translation="MTAHVAEAAEARGRVVLHLHTVNPHAVAIEAAVRVAQAFQSGIE
                     SLFVEDAQIFDLASFPFAREISFTGRRSRAISSDELEHEFRLAFASIQRRIAELARAA
                     EVPIRQRIVRDDPIAALASTCAECGPWNVVALAEPIGTMSGESLRQLFEAVSDATGLV
                     IVGPHARRTSGPVIVAVEDIDRLPSMVRAGERLVSATGGSLVILLIADDEERLHWMEG
                     QARLLLGERTDAQIVRASSARGAAEVAAEALRRLKGGFVISQFGGLVVPADRDLRALA
                     ASLECPILLVR"
     gene            39556..40926
                     /locus_tag="VF226_09215"
     CDS             39556..40926
                     /locus_tag="VF226_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7074206.1"
                     /translation="MTAISFQQHIGSRARLRSLVERLWREVTPPRAVAGVAVAVVLVL
                     WLAGGMATWVALVTIGLIAAVEVAGSRGLGPAPRRREAEPLPEGTAASSETAVGVPDV
                     SWRAVISAMPDPALALDQDGIIVHHNAGVADLFPRVRVGQPISGVIRSPELLAAIEKD
                     REPEDVTVVELQNRVPVERRVSAIVTALPLSADAGRNRALLVTFRDITDQEKLAQMRA
                     DFIAHASHELRTPLASLRGFVETLQGPARDDPKARDRFLEIMAAQATRMTRLIDDLLS
                     LSRIEMHVHVPPRGIVELNEVVAFVAQSLEPVAEAAGISIAVEPLATPARVRGDREEL
                     VQLFQNLIHNGIRYGKEGGHVRVRIAREPLAGGRGQRISLAVQDDGIGIPPEHLPRLT
                     ERFYRVSASASREKGGTGLGLAIVKHIVTRHRGELRIASKPGEGATFTVLLDELPAPA
                     ELSPMR"
     gene            41078..42115
                     /gene="pstS"
                     /locus_tag="VF226_09220"
     CDS             41078..42115
                     /gene="pstS"
                     /locus_tag="VF226_09220"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX7074207.1"
                     /translation="MKLAAAGVATWGMLAGAAWAADNITGAGASFPYPVYTKWADAFH
                     KETGIGVNYQSIGSGGGVKQIKAKTVTFGASDAPLSGKDLEASGLAQFPMVMGGIVPV
                     VNIDGVAPGQLVLDGPTLAKIFLGEIKRWNDPAITKLNADLKLPDRAIAVVHRSDGSG
                     TTYNFAYYLAEVSPEWKSEVGVSTALQWPVGIGAKGNEGVANNVGQTKNAIGYVEYAY
                     AKQNKLSHVRMVNKAGKVVSPDAESFQAAAANADWKSVPGYGVILADQPGDRSWPMTA
                     ATWILIHKSPADPAAVEKALKFFAWAYANGDTAALELDYVPMPDNVVSEVQAMWAAEI
                     KDGSGKAIFTQ"
     gene            42280..43227
                     /gene="pstC"
                     /locus_tag="VF226_09225"
     CDS             42280..43227
                     /gene="pstC"
                     /locus_tag="VF226_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7074208.1"
                     /translation="MERQRVLNRLKLWDALFARLTFAAALLVLIVLGAVFVSLIEGAW
                     PAFRTFGLKFFVTEAWNPVTEKFGAVAPIYGTLVTSFIAMLIAVPIGLGIAIFLTELC
                     PYALRRPIGIAIELLAGIPSIIYGIWGLFFFAPFFQQTVQPALISAFRNVPVLSELFA
                     GPPYGIGILTAGIILAIMVLPIISAISRDVFETVPAVLKESAYGLGCTRWEVVRSVVM
                     PYTKVGLIGGFMLGLGRALGETMAVTFVVGNAHRISASILAPGTTISATIANEFTEAV
                     SDLHTASLIALGLILFLITFTVLALARLMLLRLEVKAGR"
     gene            43231..44082
                     /gene="pstA"
                     /locus_tag="VF226_09230"
     CDS             43231..44082
                     /gene="pstA"
                     /locus_tag="VF226_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7074209.1"
                     /translation="MILDQARFAQRKRRSFIVMALCWGAAAAGLGALLVILGTLLSKG
                     LGGIRPEVFTQMTPPPGSDGGLLNPIVGSLIITSLGVLIGTPIGILAGTYMAEYGRYS
                     KLTSVIRFVNDVLLSAPSIVMGLFVYAVMVAPMGHFSGLAGSVALSLLVIPIVIRTTE
                     DMLLLVPNALREAATALGMPRSLVIRRVAYKAARAGIITGVLLAVARISGETAPLLFT
                     ALNDQFWSTNIFAPMASLPVVIFQFSLSPYEDWQRLAWVGALIITFAVLALSVVARWL
                     GTSRVAK"
     gene            44079..44867
                     /gene="pstB"
                     /locus_tag="VF226_09235"
     CDS             44079..44867
                     /gene="pstB"
                     /locus_tag="VF226_09235"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7074210.1"
                     /translation="MNIMPTVTDQALTEKISVRNLDFYYGESRVLKSINLPLYEKEVT
                     AFIGPSGCGKSTLLRVLNRMYDLYPNQRAEGEVLLDGENILSPKQDLNLLRSRIGMVF
                     QKPTPFPMTIYENIAFGVRLYEKLSKREMDERVESSLRRAALWDEVKDKLNASGLSLS
                     GGQQQRLCIARTVAIKPDVILLDEPCSALDPISTAKIEELIDELKRDYTIVIVTHNMQ
                     QAARVSEHTAFMYLGELVEFGLTSEIFTNPRKRQTRDYITGRYG"
     gene            44901..45611
                     /gene="phoU"
                     /locus_tag="VF226_09240"
     CDS             44901..45611
                     /gene="phoU"
                     /locus_tag="VF226_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7074211.1"
                     /translation="MKEHIVRSYEEELALLDRRIAQMGGLAEHALGHAFDALEKRDPQ
                     LAESVIKSDKAIDQLQRDIEGQVITMIARRQPMADDLRHVVAALRITSDLERIGDLSK
                     NTAKRALAVSHEPYPKPIMSGLRSMVERAMRQLKDVLDAYAERNSDLALTVWREDEQI
                     DAMYNSVFRELLTYMMEDPRNIGLCTHLLFGAKNIERIGDHTTNIAESVYYLVHGISI
                     TEDRPKDDQTSSTMISSD"
     gene            45634..46329
                     /gene="phoB"
                     /locus_tag="VF226_09245"
     CDS             45634..46329
                     /gene="phoB"
                     /locus_tag="VF226_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEX7074212.1"
                     /translation="MSVKVLIVEDEAPLAELLRYNFEAEGFEVAHAATGEEAELLIAE
                     EKPDLIILDWMLPSVSGVELCRRVRARQDTRALPILMLTARGEESDRIRGLSTGADDY
                     VVKPFSLPELMARVKAILRRTAPERMADVLRAADIELDRTAHRVTRGNREVRLGPTEF
                     NLLEFMMQSPGRVFSREQLLNGVWGRDAYIDERTVDVHIGRLRKALVRGKERDPIRTV
                     RGAGYVFGERRTA"
     gene            47067..47888
                     /locus_tag="VF226_09250"
     CDS             47067..47888
                     /locus_tag="VF226_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX7074213.1"
                     /translation="MSAKTSATVEKTRPAEKPVEKSALPPAAMRVGQPAPTQQRRAMT
                     AAMAKAAQPKISAPVTPKDVAARTKEARQEAQAAKKSVSARHGFKTNEYIVYPTHGVG
                     RIVGIEEQEIAGMSLELFVITFEKDKMTLRVPTGKLQSVGMRKLASEDVVKKAMDTLK
                     GRARIKRTMWSRRAQEYEAKINSGDLVAIAEVVRDLYRSESQPEQSYSERQLYEAALD
                     RMAREIAAVEKLDEQAAVQRITEVLAKSARGRRAAEEAEAEGDDDGEADPAVRAA"
     gene            complement(48074..49129)
                     /locus_tag="VF226_09255"
     CDS             complement(48074..49129)
                     /locus_tag="VF226_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7074214.1"
                     /translation="MTAQPAAHRNGLHTTDVSKPTGVDSRLKSGRAEAVRCAIRTVEI
                     EAEGLSALAGALKHEMASDFAEAIRVISASRGRVIVTGIGKSGHVGQKLAATFASTGT
                     PAFFVHPSEAAHGDLGMVTAEDVILAISWSGETVELKNVITYSRRFKVPLIAITSRRE
                     SALGRSSDVVLELPRAKEACPHGLAPTTSTTMQLALGDSLAIALLEAKGFTAHDFKLF
                     HPGGSLGAQLKYVADIMHKGEELPLALEGESMSAALVTMTQKSFGCLGVIDTNGKLVG
                     IVTDGDLRRNMGERLLSARAADIMTRKPKTVTPDTLASAALELINAARITALFVVEKG
                     KPVGIVHVHDLLRVGVA"
     gene            49352..50452
                     /locus_tag="VF226_09260"
     CDS             49352..50452
                     /locus_tag="VF226_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX7074215.1"
                     /translation="MTLGAHPFFQPEKRPHVEGDFRREEVALANRNSGLPLEALRHDL
                     TPIGLHYLLIHFDIPYVEAPERWNVKVGGRVSNAIEVPLSEIKRLPQRSLRVTLECAG
                     NGRAKLTPRSQSQPWGNGAVGTAEWTGTPVRYLLERAGMAADAVDVAFFGADRGFDAG
                     VEHDYGRSLTPDLAMSDDVLLVHTMNGQPLLPQHGFPLRLIVPGWYGMASVKWLNRIE
                     VLDRPFDGFQQVRTYVYRQHSGDEGVPVTHMRVKSLMVPPGIPDWYTRARLVERGPVE
                     LYGRAWSGGGVPIAMVEVGVDGAWQEATLDPPAGQYAWRGWRFTWDAQPGEHELMCRA
                     TDEAGNVQPLDAPWDSGGFGNNVVHRVTVTVR"
     gene            50494..51240
                     /locus_tag="VF226_09265"
     CDS             50494..51240
                     /locus_tag="VF226_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX7074216.1"
                     /translation="MPSSAQSAQPASSAIEVLIWRGRENGHYQRFTVPLRESQTVLDV
                     VTWVQRNSDPTLAYRFACRVGMCGSCAMTVNGRPRWTCRTHVANVVEYGRLEIAPLAN
                     LPIIKDLATDMSRFFEKWQQARARFEPSRTRHEPVETISPNTAERMAADDAIECINCA
                     VCYAACDTVRWNDDYLGPAALNRAWTLVNDVRDAGQKERLKAVSDVGGCQSCHSHQSC
                     AELCPKSLNPTASIASLKRRAMWAYLKGEL"
     gene            51464..51793
                     /locus_tag="VF226_09270"
     CDS             51464..51793
                     /locus_tag="VF226_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX7074217.1"
                     /translation="MTLRLYVLQRLTAALMVPLIIGHLIVIFYATSNGLSAAEILGRT
                     RGSIGWALYYGLFVILASVHGAIGVRGVLREWTRLTPGALDAIMWAFGLLLAILGARA
                     VAAVALP"
BASE COUNT         9558 a        16090 c        16695 g         9614 t
ORIGIN      
        1 aagctgtcgg tccgacgacg gatgatcgat agcgtcggac tgaccgcgcc cgcttataag
       61 gcaatcagcc cggcgttgag gaagccttca agaagctggt taaccgctgg tgccgtgccg
      121 cttcgttcgc ggatcgaccg tgtccggagc gtcttcggat cagagccaca gcaccttccg
      181 ccgcagctcg aagtcctcgc tgagcgcctg ggaggggccg gtatggatca ctcttccgcg
      241 ctcgagcgca acggtccggt cggacagcgc caatgctaga tcgaggtggt ggtcgacgat
      301 gacgatcgag atctgatggc gcaggctgtc gaaggattcg aacagctcct cgatgacagc
      361 aggagccagc ccttcgaacg gctcgtccag cagcaggaca cgtgtgtcgc cgatgagcgc
      421 gcgggccacc gcaaccatct gctgctcgcc gcccgagaga tagtccgccg gcgtgcgcca
      481 gcggtccttg atccggggaa agaaggtgaa gatcttttcc tcgtcccaga aggtgcccgc
      541 gccgttgcgg cgcttgagcc ggcccagctc catgttttcc gccaccgtca tgccggcgaa
      601 caggccgcgg ccctgcggga catacgagat tccgcgccgt gtgatcacgg ccgatggctg
      661 gccggcgagc tcctctccgc cgagccggat ggagccgcgc ctcggcgggg taatcccaac
      721 gatgctcttg agcagcgtcg acttgccggc accgttccgc ccgagcagtg ccacgatttc
      781 gttctcgcgt acgctgaacg agacggagcg caggacgtgg ctcttgccgt agaacgtgtc
      841 gaccccttca agcgtcagaa gaggctcgtc cttggcggag gtcgcccggt gcttctcgct
      901 gagcgcgtgt gcgccggagc cgatgtagac ctcctgcaca cgctgcgagt tgcgcgcatc
      961 atcgacgctg ccgtcgacca gcacctgtcc ctcgttcatc accgtgacag catcggcgat
     1021 ctggaaaacg cgatcgatat catgctcgac cagcagcacg gggatatcgg cggagatgcg
     1081 tttgatcaga ttgccgaccc gctcccgttc ggccgcagcg agccctgcca gcggctcgtc
     1141 gaggaggaga acgcgtggtt tcgtcgccag cgcgagagac atgtcgagca ggcgctgacc
     1201 gccgtaagac agcgagcccg cttcggcccg ctcgacgcct ttgaggccca tcgtccgcaa
     1261 aatttcggcc gtctcggcgt tgacatcggc aaggtcgagc gcggccgtcc agacgtcgaa
     1321 gcgatggggc gagcgcgctt ggaccgccag ccggacgttc tcctcgacgc tcaaggccgg
     1381 gaagagattg gtgatctgga aggagcggcc gatgccggcc tgggtcagtg cctccgggct
     1441 cagccgcgaa acgtccctgt cggcgagctt gatctggccg gcatcgggtg gaaagagacc
     1501 ggaaatgaca ttgaacgccg ttgtcttgcc ggcgccgttc gggccgatca gcgcgtgcaa
     1561 tgtccggtcc cggagagcaa agctggcgcc ttgaacggcc ttgatgccgc cgaagctctt
     1621 ggcgatggca ttgacgacaa ggattgtgcc agccgtgtgg gtcgcaggct tcagaaaatt
     1681 cggcaggtcg atctcgccgg cctggcggcc cgccatcgcc gcgtcctcaa ccagcttcgg
     1741 gcggaaaggc gccgtcagcc gctcgtagac acccacaaga ccctcgggcg agaagacgac
     1801 gaacccaacg aacatcaagc cgaaatagag aagccagttg ggcgtgatgc tggagaggaa
     1861 gtcgcggaat ataacgtaga acaaggcacc gagtgcaggc ccgagaaagc tccgcattcc
     1921 gccgatcacg accatggcga gcagctcgcc cgaaaacgcg atcgaaatgg gctcagccga
     1981 ggtcatcctg ttgttgaaca gcagcagcat gccggccagt ccggtcagcg tcgcggaggc
     2041 gacgaacgca aggagcttgt agcgatctgt tggataaccg atgaagcggg cgcgctgctc
     2101 gttctcacgg atcgcgacga gcacgtgacc gaccggtgag cgatggaatc gccagagggc
     2161 atacagcacg ccgaatccga tcagtgccat gagaacgtag tagttcgtcg cattctcgaa
     2221 atcgatgccg aacgcctgcg ggcgccggat gccgccgagg ccatcctctc cgccggtcac
     2281 ggacgtccag cggaaggcga cggcgtagag catggccgag agtgccagcg tcagcagcgc
     2341 gaaatagacg ccgcgccggc gcaggatgag aaacccgaag cctgccgaaa cggcaacgat
     2401 caccgccagt ccgagcagga tcggcaggat gaaggcatcc ggaaagagat tccgctgcgc
     2461 aaggcctgcg gcgtaagcgg caagtccgaa ccaggcaccg tggccgaagg aaacgaggcc
     2521 ggtatgcccg acgaggatgt tcaatgccat gcaggcgatc gcgaatatga cgacttccga
     2581 cgccgacgtt attccgagcc cgatggcgga gaggacgaac ggcagcaata tcagggcggc
     2641 agcggtgatg acgagcggtt tccggtcgag cttcatggcc gtcgctcact cgaatttctg
     2701 aattcgctcg ccgaacagac ccctcggtct gaacaggagg acgagcagca tcatgaaata
     2761 gatgacggcg gtcgaagcct gcgcgtagcc gaaggcaatg gtcatgccct tcaccaggcc
     2821 gaccagcaac gccgcgacga tcacgcccca gaatgagccg agccccccga tcaccacgac
     2881 gacgaaggct ggcgttatga tctcctgccc catagccgga tggatggagt agatgggcgc
     2941 cagcagaacg cccgcaagcg cggcaagacc aataccgagc gccgcgaccg ccgccatata
     3001 gggctgcagc gaaataccga gcgcgcccac catatccggg ttctggacac cggcccggac
     3061 cacgcggccg aatgctgtct tgttgagcag cagccacaac ccgatcacgc agacggccgc
     3121 gactccgagc aggagggtcc ggtaataaga gtagatgaag tcaccgacga tgatcggccc
     3181 ccgaagcgcc tgcggcaggc caaaggacat cggcggcgcg ccgaagatca tgcgcaagct
     3241 ctgctccgcc accatggcga ggccgaaggt gaggagcagc gagagaatag gatcgcttcg
     3301 gtagaagcgc cggaacagaa agcgctcgat caaaatgcca agcagcgcga cggcgatcgg
     3361 agagaccacc agcgccccgc tgaagccgag gtgaggggcg attaccacgg tcaaataggc
     3421 gccgatcgca taaaacgcgc cgtgcgccag attgatgatg cccccgagcg agaaaatgag
     3481 ggacagcccg agcgcgatca aaaggtaata gacgccgtcg agcaggccgt tgaggacctg
     3541 ctgggcaaag agtgtcgcgg acacttggaa gtggctcctg aaagtccagg cactgccccc
     3601 tctccccgag ccggggagag ggggcagtgc tgccggctat gcggacatgt tgcagacgtt
     3661 ctcttccttg gtcgccgcga tgacctcgag gtcctcatcc ggccctggcg tcggcggtcc
     3721 gggcttgtag atgtcccact gattcttgat ttcggaggcc ttcagcgcct cgacggcata
     3781 catttcatgc atcagctgat gatcgtacgc ccggaaataa ccctcgcgcg ttttcagcag
     3841 atcgaatttc gctccctttt cccaatgctc gatcagcttc ggcgcctcga tcgatttgat
     3901 ctcgttcatc gacttggcaa ggatgtgcat ggccacgtag tcgccccacg cctggttgtc
     3961 gggcgggcgg ccgtacttct tcgtgaaggc ggcgacgaag gccttcgacc ctggcgtgtc
     4021 gacgaggtga tgccagacca gtggccacgt gccgaagaaa ttgccctgtc cggcgcccca
     4081 ggcgacggct gtatcgaagc caaatcccgc aaccgggaaa tcaaggccga attccgaaaa
     4141 ctgcttcagg aagttcgtga tctggacgcc cgccaggttc gatacgacga gatccggctt
     4201 ggcatcgcgg atcttgacca ggaaggccga gaagtccgca gcatcggtcg ggaccagctc
     4261 atcggctgcg aattcgcccc cattggcttc catgaatcgt tttgcgactc gcagcaggtc
     4321 gtggccgaac gcgtagtccg ccgtcagcga gtaccacttc ttgcccttca ccatgttctg
     4381 ggccaggagc gcgcgcccga ccgttttcac gtacatcgag ttctgactct cgacgtggaa
     4441 catgtacttg ttgcagttcg agccgcgcag cgcgtcggag ttggcgccgg tattgacgaa
     4501 caggttcttg gtccgctggg cgacctgggc gatggtgagg gcagaggccg aggagatctc
     4561 gccgacgatg caggcgacct gatcgcgctc gatcatgcgt tcggctttcg ttgaagccgt
     4621 ctgcggatta accgagtcct ccttcaagag ctcaattttc cggccgagca cgccgccttt
     4681 gccgttgatt tcctctacgg cgagatctgc tgcctggacg gcgaactccc cgagcgttcc
     4741 gagaaagccg gtgcgtgggg tcaggtggcc gatcttaatg acatcggact gcgctttcga
     4801 aatggccggg aaccccagaa caccggcagc cgctgcaccg gccgcccctt tcagtacgct
     4861 gcggcgcgac agctttcgct tgatggacat ccgtttcctc ctgaatctgt tgccggcttg
     4921 ttctgccggt cagcagccat gcccggagcg gcgggttggc tcacacgagc tattgggccc
     4981 cgtccgagca tggcgcaatt agcgtgatcc aggcgtgaag gcttgtccag aggctttctg
     5041 agttagctga ttatcattat cctgtgcgag gcagctggtg ccgttcgtcc gccacttcga
     5101 ggccaaggct ccttgcttcc agcgaaggcg gactgggacg gtgcatgggt ccggccagga
     5161 gcgggcggcc gatgtagccg tcgagctctc cgccccacca cagccgcaat ggctccacga
     5221 agcttacctg ccggcggccc gtctcacaat cgaagtagca ggggtgatac ttccacagcc
     5281 gccatgcggt caggtagcgg atgcgcgtca ggcgcaccca gccgatcacg gcgcgtacgc
     5341 ccggcagctt tttgagccag gcaacgagcc tccgatgcca agtgccgaat gttttccaat
     5401 accaaacgcc gaatatgaga tccgcggtgc ggatccagag cttgcggacg acggggacga
     5461 ggaatgcgat cggaaaaatg accagcaccg tgtgcaggta gacctggctc gcgatgagcg
     5521 ccgccacgat caccagcttc cagagaagcg gaagattttg ccatctggtc ttgacggagt
     5581 tgatggtggc cctgagcttg ccgcgccagc cgcgcgcctt ttgaacggcg tcctcgctga
     5641 gcaccacgct ggcagcatag ccgaggccga tgccgcctat gacgttggca atgcgggtcg
     5701 cccgacgcca gccgacgccc ttggcgatta acccgagcag gccgccgaag aggcggaata
     5761 ggccgttgat gataaaagca aacccgttcc agaacacccg cgcagctgcc gccagtgcct
     5821 cctgcccggt aatagtgccg atggcaatga aggccagcac ggcgatcagc gcaaagctga
     5881 gataccagcc gatgccgtcc tttcgctctg tcgaaggcct ttcgtccgcc acgaccctcc
     5941 ccctcgaagt cgccgccatt gtttgtggta ggaggggttt ttgccgaaaa tgcgggcgtg
     6001 ggccagcctt tcgtcgcatc gctcaacagc gcgacgagtc agcttttggc gagatgacgg
     6061 atgaacgcgt gcaggcctgc acgtctcagg ccttgggcag tgaagcgatc tcttccagaa
     6121 aacctaccag gttttccgtc atgtggtgaa catgctccgg cggctcgcgc cactcacgga
     6181 tcttgagctg gatcggatga tcgtaatagc tcgagtgcac gagcaccgtc gtcatgccga
     6241 gcgcgtgcgg cgcctcgagg ttctgcggca tgtcctcgaa catggcggct gtctgcggct
     6301 ccaccccgtg gcgacgggcc atgcgatcga aggcgtccgg gctcggcttc ggcacgtact
     6361 cgcaggcggc gatgtcgcag atgtcctcga agaggtgcag cacgccgagt ttcccggcca
     6421 cgcgctccgc atggcggcgg gagccgttcg tgaagatcag acgccgccct gggagcttct
     6481 cgatcgccgc agccagctcc ggaagctcag gcactgcgga cagatcgatg tcgtgcacgt
     6541 agtcgagaaa cggctttgga tcgagcttgt gaacggccat caggccgcag agggtagtcc
     6601 catactgccg gtaatagctc ttctgcaggt gccgcgcgta ttcgaaggga acgccgaggt
     6661 actgtgcaat gtactcgccc atgcgccggt ccacctgggc gaacaggttg cattcggccg
     6721 gatagagcgt gttgtcgaga tcgaagatcc acgtgcttgt cgtctcgaat ccgcgccggc
     6781 gcgcggcagg catcactgcc gctccagcct tacgggcggc ttcgggtgac gtggcgttca
     6841 tgacagcgca ggttcctccg aggtcccgac cagggtgccg accccgtgct cggtaaacag
     6901 ctccagcagc acgcagtgcg gcacccggcc atcgatgatg acgaccgcct cgaccccggc
     6961 ctcgaccacc tcgatgcagc cctcgatctt ggggatcatg ccgccggtga tggtgccgtt
     7021 gcggatcagc tcgcgtgcct tggcgaccga aagctcggga atgagcttct tgtccttgtc
     7081 gagcacgccc tcgacatccg tcagcagcag gaggcgcttc gccttcatct tcgcggccag
     7141 cgcgctggcg aaggtgtccg cgttgatgtt gagcgtttgg ccgtcgcggc tgacgccaac
     7201 cggggcgatc acgggaataa ggtcggaatt cgagatcacc tcgacgatat ggggattgac
     7261 ctcctccggg tcacccacat acccgatgtc gaccgccttc atgatgttcg attccggatc
     7321 gggcatctcc gtgatccggc gggcgatcat caggttggca tccttgccgc agatgccgac
     7381 ggccttgccg ccctgccggt tgatcgccga tacgatctcc ttgttgatgg cgcctgcgag
     7441 caccatctcg accacctcga cggtcggctt gtccgttacg cgaagcccgt tgatgaactc
     7501 cgatttgatc tcgagcttct tcagcatcgc cgcgatttgc gggcctccgc cgtgcaccac
     7561 gatgggattc acgcctgaga gcttgaggta gacgatgtcc tgcgcaaagg cgtcggccag
     7621 agcggcatcg cccatggcat ggccgccgaa cttgatgacc acggtttgat tgtcgtagcg
     7681 ctgcaggtaa ggcagcgcct ccgccagaat ctgcgccttg gaatgagccg aaacggcgcc
     7741 gtgcgcgcct gggacttgca ctttgtcggt cctgccagcc atgtcgtcgt tcccttgccc
     7801 ctgggcgagt gcagttatag cggctcggcg ctgcccctca atggaattcc cccacaatcc
     7861 tgggatgcgg agccgcggat gctgcaccgg cgaggccggc atgagaagtt gggacagccg
     7921 gatacgtgca gacgggagcc cgatcggtta agcttcgcca cgagtcgagc agatttagct
     7981 gccattgacg tggggcggct ccagtccgaa ggacgagccg aaggggagca cgaatgtcgc
     8041 agtcgtacgc cgaagctctg agctgggtgc cgaaatcgcg cggcctggcg acgacgcttg
     8101 cgcgcgcgtt cgactatgcc gcgagccaat cgcataaatc cgtgctcctg gagcatctgc
     8161 tcctggccct gatctccgat gccgatgcgt cagcggtgct gcaggcatgc cgggtcgacc
     8221 tcaaccggct cgatagcgat gtgacgaatt atctggcggg ggtcgacgaa cgcctctcgc
     8281 cgggcgacac ctcggctccg gtcgcctctc ccgagctcct gcgcatcgtc gagtatgcct
     8341 cggccgctgc tcgtcagagc cgccggcgcg aggtcaacgg cgccatcgtg ctggctgcaa
     8401 tcgtcggcga tgggcgcagc cctgcggcca gcatgctgcg agagcaaggg ctgacgttcg
     8461 aggaagccat caaggcgctg cagaaagcga atgctgcggc acgcgagccg gggacccggc
     8521 cggcatcggt ttccgctccg cgcccgaggc ctgcagcccc acctccgcag gcggctcctg
     8581 cagcgccctc gtacccggac gagcaggctg cagccgaccc gcacctgtca ccgcagcagc
     8641 cggggcctcg gctttcgcat ccggaacagc atccagaccg ggatcggcct gccgcagcat
     8701 ctgctgtgaa tggagtggcc ggggggcaga cgaccgaaga aatccttgct gcggcgcgcc
     8761 gtcgggtcgg ctcaggactg actggcggcc aaccgggcgc aaagccggaa actcaggatg
     8821 cgcctcggca gcagcctgaa cgtcatccgg ccgatgatgt cgctcatcaa gagcagcccg
     8881 aggatgagag agacgatgct atcgcctcgc aagagcatag tgcgggtcca ccgcccggcg
     8941 aggatcggca tcttgtgccg ccccagccgc agcccccggg ccgaccccag gggccaatgc
     9001 ggccacacgc tgcgccgcag ccggcggatt actcgcgatc gtatccaccc cccggtttcg
     9061 gccaaaacgc cccggcggag gatttaggca cgcaggcccc gcagccacgg cgctcggatg
     9121 ctccatcccg caccacgcct cctcctcgtg gggcgcctgc gccggaatac gggccgacgc
     9181 tcgattggcc gcgaccggca ccgtcacctc aggatagcta ctatcgtccg caccgccccg
     9241 acaccgacga cacggagcag gtgcgacggc agcctactgc gcacccacag caggaacgat
     9301 ttcatccgcc acctccgccg ccaccccatt ttccgggcca tccagcagcc gggccatcgc
     9361 ggcccgggcg tgcgccttgg cccgagccac cagctccagc gtatccacag cagggcacat
     9421 atggatcgcc gcaaccggct gcggcgcggg ctcagcctga gctgaggggc agggctccga
     9481 acgcggatga ggccccggct acacaggttt tcgcggggca gctcgtcgag aacatcccgc
     9541 gcacgatgac cgtcggcgtg ccggagctgg tcgaggtcag catcgccaag gccgagttgg
     9601 caggtcttgc cgagaagctg caggggtccg gcgcagccta ccggcacgac atcgtggtca
     9661 ccaaagcgat gtcagtgcgc ctgcgtgcgc cggaaggcgg cttttggatc gagacggcgt
     9721 ccccggaaac acagtggatc gagaattcgc tcggcttgct ttcggacgac tacgcgagct
     9781 ggcgctggac ggtgacgccg cagcggcgcg gccgtgcacg gctgcagctc atcgtctcgg
     9841 cgcgtacggt cggcgcagac ggcctggctg ccgagaccgc gctgcccgac caggtcatcg
     9901 aggtgaagat ccgcgcgaac tacgggcgca cggctgttca gtggggtggt tggatcgcgg
     9961 cggcggtcct gggcggcatt tttgccaagt tcggcgaagg gcttttgagt ttcggcggca
    10021 cagtgctgag cctgctcggg atactttgaa ccgcagcctt gatcgtcagc cgctgctctg
    10081 agtgaggcaa agctcggcga tcctggcgcg aaggacgtcg atgccgacgc ccttggctgc
    10141 cgatgtaacg atcacctccg gataggcggc cggatgtgcg gtgagcgcga gcttggtcgc
    10201 cgcaacgact ttctccagct cgctgcggct cggcttgtcc gctttggtca gcacaacttg
    10261 atatgagacg gccgcttcgt cgaggagccg catggtctcg cggtctggtg gcttgaggcc
    10321 gtgccgcgag tcgatcagca ggaatacgcg cttcagcgtc gggcggccgc gcagatagtc
    10381 acgaacgaga ccgatccagg ccttgacctt ctcctttggt gcgcgagcga agccgtatcc
    10441 cggcatatcg acgagataga gcgggacgtc ggctgttgcg aagaagttca gctcctgcgt
    10501 gcggccgggt gtattcgacg tgcgtgccag tccgtgctgc ccgacgagcg cgttgatgag
    10561 gctcgacttc ccgacgttcg agcggccggc aaaggcgatc tcagggcggt ctgcgtcagg
    10621 caggaactcc agagagggca cgccccgcac gaagccccaa gggcggccga agagcgctgc
    10681 gccagtggcg agccattcct gggtcaccgc ttcgctggtc tggaattcgg cctctgatcc
    10741 gtgcggtggg ttcgttccca taaccagctt gaatagagct tcggcaaggt gaggagctga
    10801 ttatcgcttc gccttccgct tcttcggctc cgctttcggg gcctcggctg cgccctcggt
    10861 cggcgactca gagccatccg ctgcggcatc ggcttcggtt ttctcggtct cgctggcttt
    10921 ctccttggcc tttggcgccg attgagccgg cgtcttcttt tcgacagcct tcgttccgga
    10981 aacgagaccg acgaccggtg cgaagttgcg cttcaggttg gcgaggatcg ggacctccac
    11041 gccctggcgc ttcataatca gccattgctg gcccagtgag aggatgttgt tccacgccca
    11101 gtagatcacg agacccgccg ggaacgccgc cagcaggaac gtgaacacca ccggcatcag
    11161 attgaagatc ttctgctgga tgggatcggg ctgctgcgga ttgagctgca tctgcagcca
    11221 catcgtgatg cccatgatga tgggccaggc gccgatgtgc aggaaatccg gcaccgcgaa
    11281 cggcagcagg ccgaacaggt tgaagatcga ggtcgggtcg ggtgccgaga gatccttgat
    11341 ccagccgaag aacggtgcat ggcgcatgtc gatgctgacg aacagcacct tgtagagcgc
    11401 gaagaacacg gggatctgga tggcaatcgg caggcagccg gccagcggat taatcttctc
    11461 cttcttataa agcgccatca gctcctgctg ttggcgcatc ttgtcatctt tgaagcggtc
    11521 ccggatgcgc tccatctcgg gctgcagctt cttcatcttc gccatggact cgtaggactt
    11581 gttggcgagc gggaagaagg caatcttgac gatcacggtc accgccagga tcgcgaggcc
    11641 gaagtttcca agcagtccgt agagccagtt aaggagatga tagagtggct tggtgatgaa
    11701 gtagaaccag ccccaatcga tcagcaagtc gaactgcttg atgtcgtacg tggtggcata
    11761 atcctgcacg gtgccgactt cacgcgcacc ggcaaagaac attccctcgg tctcggactt
    11821 cccaccggcc ggcaacgtga cagccggcag caggtaatcc gcctggtagg aatcgggttg
    11881 ccccgcggtg ccggcctggc ccgtcatact ggcgttgtag gacacgctct ggtttgggat
    11941 gagcgttgca gcccagtact tgtccgttat gccgagccaa ccgcctttcg cgtcagtgac
    12001 ggtcgctccg ccattcgcct tgagcgcgtt cgcataggtg atctcgtgca gcccggcttc
    12061 gccgagcacg ccgatcaggc cctcgtgcag gatatagaag ccttgaacgt gcggcgtgcc
    12121 ttcgcggcgc aggcgcgcat agggatagag tgtaaccgga cctccggtgt tgttctccac
    12181 cgcgtcggtc accttgaaca tgtacttgtc gtcgatggag atggtgcgcc ggaagatcag
    12241 cccctgctgg ttgtcccaga ccagcgtgat cggattgttg ggcgtgagcg gtccgccttt
    12301 ctcgacctgc caaacggtgt ccggcccggg caggctcagg ttggtgccgc tgggcgcgac
    12361 ccagccatat tcggcaaagt agctatcggg acctccaggc ggcgagagca gcacgatgtt
    12421 cgggctgttc gggtcgacgg tctcccggta gcgcttcagt acgagatcgt cgatgcggcc
    12481 gcccttgagc gcgatggagc cgcgcacaga cggcgtgtcg atctcgacgc gcggcgattc
    12541 cgccagtgcc gcctgacgcg tgggggcgac ggcgggggca gacggcggcg caacggtgcc
    12601 tggagccgcg ggtttcgcag cctcaggtgc cgtctccgtc ggtgccgggc ggcctgcctc
    12661 gttccgcggc gcctgtggcg tgccgaagat catttgccag ccgagcagga ccgcgatcga
    12721 aagtataatc gcgatcaaca ggttcttctg gctctcttgt tccatgggcg tctcgatcgg
    12781 ggccgttctt cagaacgatg gctgcttgtg tagggcgtgt gtgccggttt ttcgagccga
    12841 cggaaagtgg cagcaggtcg cgcttgccga gttttcaatt accggacccg cttggatctt
    12901 cccgagcgat gtatacgcgc cagggcctct gccaagtcgg ccttcaggtc cgcataggct
    12961 cgctcggcgg ctacgggccg agcgacgatg acatagtcga agccggcgcg cgcggagctc
    13021 aagagctccc tgaccgccgc cttgagccgg cgccgcaccc tgttgcggac gacggctttg
    13081 ccgaccttct tgctcacggt gaagccgaac cgggggcgtt ctccttccga gctgccacct
    13141 tccgggctga tcgcggcccg cggttttgcc tcgagcacca gggagggggt cgcccatctg
    13201 agaccgcctc tgaccctgag aaactccgat cggcgcttga gtgtcgtcag ccccacggct
    13261 tgcttctgcc cattgcaggg tctgccgcag ccagccgcgg gcggcggcct ctgccatcag
    13321 gccgagaggc gcttgcggcc cttcgctcgc cggcgcgcaa taacctttgc cccgccgggt
    13381 gtcatcatgc gcttccggaa gccatggcgg cgcttgcgaa caaggcgact cggctggtag
    13441 gtgcgtttca cgttctctct ccgtatcgca gtcgatcact gggctggccg agcggagagg
    13501 gcccgaacgg ctcaactcgt gcgcgtcacc cgagcggtca cggttccacc tgctctcgtg
    13561 atcgggaatt tgaccgcgtg cttatagggg ccggcaggcg ccaagtcaat tagaggggaa
    13621 tcgcgtgcgc cccctcttgg ctttatcctt caaatgattg cgaggtggtt cacgtctgac
    13681 ccgacggctg ttcccttttc ggagggaaac gctcaccttc cgagctgcgg tggccgaggc
    13741 ttccgaatct gcggatctac aaattctccg gtccgcgaac gcgtatcgat gaattcgaac
    13801 acaggacaac cgagattgaa cccgaggcgt tggctcccgc tcgtcctgct gctgactgcg
    13861 accgggcttg ccttcggcat gggctggcac aggcttctga ccttcaaagc cgtcggtctc
    13921 aactatgaag ccctgtcgga tttcatcggc gcccacctgg tagtcgctct acttctctac
    13981 gtgggggcct atgtagcggt cgtggccttg tctttacccg gtgggctcgt gatgacggtc
    14041 gctggtggcc tgctgttcgg atgggagctt gcggctcccg tcaccgtgct gtcggccacg
    14101 atcggggcga gcatcatctt cctggttgcg aagacctcac tgggtgagcc gctggcggcc
    14161 aaggcggggc cgtggctcgg caggctgaga gcgggctttc aggagaacgc gctgagctac
    14221 ctgctcttcc tacgcctcgt gccgcttttc ccgtttttcg tggtcaatct ggccccggct
    14281 ctgctcgggg tgccgttcac gacattcgcc atcgccacgc tcctcgggat catccctgga
    14341 actctggcct tttcggtggc cggctccgga ctggcgagcg ttatcgaggc ccagaaccgg
    14401 atttaccagg attgtctcgc gcgcgctcct gccagccagg aagcgaattg tccatatacc
    14461 gtggacgtcg gtgccctcgt gacgccggag ctcattgccg cgttcattct gctcggcctc
    14521 atggcgctcg tcccggtggc ggtcaagaaa tggagcaagc ggaatgctga agcgtgatgt
    14581 cggtgatggc ggtgcgggga agatcctgcc ttcggccagc aagagcgatg tcataaccgc
    14641 tgacttgtgt gtgatcggtg ccggaccggg tgggctttcc gttgcggcag cggcagccgc
    14701 cttggggcgc agcgtcgtgc tggtcgagaa gcacaagatg ggcggcgact gcctcaatta
    14761 cggctgcgtg ccctcgaaag cgctgctcgc cgccgcaaag cgggcgcaca taatgcggac
    14821 cagtgacaaa ttcggcattg cctcggtcag gcccgaagtc gattggcagg ccgtccggga
    14881 tcacattcgt gacgtgattg ccgaaatcgc gccgaatgat tctgttgaac gctttacggc
    14941 gctcggtgtc agggtcatcc aggctgcggg acgtttcgtc aacaagcgca ctctgcaggc
    15001 aggcgagcat ctgatccgtg ctcgccgctt cgtcatcgcc acgggatcct cgccggtgct
    15061 gcccgagatc ccaggcatcg acgatgtacc gtatttcacg aacgaaacca tcttcgagaa
    15121 tgcttctccg atcgcgcacc tgatcgtcat cggcgccggt ccgatcggat tggagctggc
    15181 acaagcctac gtgcggctcg gcagccgtgt gacggtcctc gagtccggga cggcgctggg
    15241 caaggaggac cccgaactcg cgcgctacct ggtcaacgag ctcattcgcg aggggattga
    15301 cgttcgcgaa aaggtggcca tcgagcggat cgacggctgc gaaggcgagg ttcgcgtgtc
    15361 gatcaagacc ggaaacgcca gcgagagcat cgctggtacg catcttttcg tcgccacagg
    15421 ccggcgcccg aacatcaccg acctcaacct cgaggctgca aaggtcaagt atgatagccg
    15481 gtccgggatc aaggtcagcc aggggctcgt cgcctcgaac cgccggattt tcgccattgg
    15541 cgattgtgcg ggcggccccc agttcaccca tgttgccaat tatcacgcgg gcatcaccat
    15601 ccgcagggct ttgttccggc tgcctgccaa agtcgatgag cgcctcatgc cgcgcgtgac
    15661 cttcaccgag cctgagctgg cctacgtggg gctttctgag gagcaggccc gtctggccgg
    15721 agaaattcgg gtcctccgct ggccttactc agagaatgac agagtccagg ccgagcgcgc
    15781 gacccgaggg ttcgtcaagg tcataacgga taagcgtggg cgtgttctcg gcgcaggcat
    15841 tgtcggccag caggcgggag agctgattga gatatggtca cttgccattt cgcagaacat
    15901 gaagattaaa gccctggcgg atctggttct gccttatccg acgctttcgg aggtcaacaa
    15961 gcgcgccgca ttccgttatt atgcgacggc agccagctct ccggtcgtgc ggaaggtagt
    16021 atcgatgctg gccaagctgg gataggcatt ggaagtcgag gcgtaggtgc cgcaggattc
    16081 ggctccccct gggaagaacg gcggccgggc gaatgaagcc gtcgaggcgg tctcggatgc
    16141 tccgacgcac agtgcgtggg aaattcggct gcatggtctg ccgtcgaagc ttctttacct
    16201 gacagccttg ttcgtgatgt tggcggaggt gctcatcttt gtgccctccg tcgccaactt
    16261 tcgtgttacg tggctcaccg accgcctgac ggccgcgcgt ttggctgctt tggcagcgga
    16321 cgccgcaccc ggcggcatca ttccggagaa cgtgcgcagt gagctgctgg tcacggcgca
    16381 gcttcgttcg gtggccgtca aggccggcgg catgcggaag ctcgtgctcc ctcccgacgg
    16441 caacttcgtc gtggatgcgc aattcgatct gcgcgagccg ccggagtcga cggttctcga
    16501 gcgcttgtcg aatcgttttg cactgatcgc cgacgccgtg ggcgtgttct ttgcgccggc
    16561 gggccgcatg atcctggtcc ggggccaccc tgacacgggg ccgcgcccaa ggcttcccat
    16621 gacggatttc atcgagatcg ttctgccgga agcaaccctc aaagcggcga tggtgcggta
    16681 cggtttgaac gtcctgggac tgtctatcat catttccatc attgccgccg cgctggtcta
    16741 cgtggcgctg agcgcgatgc tcgtgcgccc gatgatgcgc atcacgcgca acatgctcgc
    16801 tttcagcgaa aacccggagg atcccagccg gattatcgtg ccaacggatc ggcgcgacga
    16861 gatcggcatt gccgagcggg agctggcgcg catgcagcgg cagctcgctc aaacgctgca
    16921 tcaaaagagc aggctcgccc aattgggtct ggccgtgagc aagatcaatc acgatctccg
    16981 caacatgctg gccagtgcgc aactcatttc cgaccgattg acggctcttc ccgacccgac
    17041 ggttcagcgt ttcgcgccga aactcatcgc gtcgctcgat cgggccatca gcttctgcaa
    17101 cgacacgctg cggttcggcc gcgccgagga ggccgcgcct cggcgtgagc tcctcctgct
    17161 gagaaacctt gtcgaggacg tcggcgaaag tctcggcctg cctcgcgacg gcatcgactg
    17221 gcacgtcgct attgatgaaa cgctgcgagt ggacgccgat ccggagcaca tgttccgcgt
    17281 cttgaacaac atctgtcgaa acgcggtgca ggccatcgaa agccaaggcc cgcaggccgc
    17341 aggcgaagtc gtggtcgagg cccggcggga aggtttgcgg tccatcatca cggttcgcga
    17401 tacggggccg ggcttgtcgg cagaagcgcg ggcgcgcttg ttccaggcct tcggaagctc
    17461 ggcgcggaat ggcggctcag ggttggggct tgcgatcgcc gcggagctgg tgcgggcgca
    17521 cggcggcacg atcgaggttc tcgacgttcc gaaaggtgcc gtatttcgca tcgaaatacc
    17581 ggatcgtggc acgggcacgc gcaactgagc cgctatgcag cgaggctccg gcctcgctct
    17641 ccgtgcacgc ggaagtgcga tacacagagc aatcggcaga gcgatgtcgc gatgattgcg
    17701 ggcccaccga cgctgcattc aggcttggct ccaccgccag gtgctctacg tcgggctgtc
    17761 gatcagcagg gtcgaacggg caaatctgtg attgtggatc cgagctcgtt tctcttgtct
    17821 gcaggctttg gggatcgcgg tttatctgta ttttctcctc tttagaggcc aaagaatgtc
    17881 gcacacgatc cttttttgtt gcaaaggtag cacaccgtaa tatttagttt atgtttaggg
    17941 catgcaatta ggttcgcaaa tgaggtgaac tcagttaagt gcccaaaatc atagagtttc
    18001 gagcccgcac ctcagttcgc gaggcaatca ggcatacgaa gagaggatgt catgaaggaa
    18061 agtcagcttc agaagatgag ctataaggag ctgttgcagc tccagaagaa ggtcgcagac
    18121 gcgatcgcga agaagagggg ttcagagcgg gccgctctga agcagaagtt gcagtcgctg
    18181 gcggagcagg ccggttttca gatcgacgat cttttcggcg gtccgcgtcg ggaacgtgcg
    18241 gccagctctt ccattaccgt caaatatcgc catcccacca accggtccct gatctggacg
    18301 ggccgcggtc gcaggcccaa gtggctggtt gccgagggtg gtgacgtcga gcgtttccgc
    18361 gtcgcttgag tcctgaccaa cataaatccg atctgaggag accaaagtca tatgaaagcg
    18421 ccagccgcca agacaaaggc gaagaaggca gcagcccccg ccaaggccgc ggagaaagcc
    18481 cctgcaaaag ctgcagcgaa ggcccctgcg aaagctgcaa ccatcacatt gaatcagatg
    18541 gccaaggagc tgtctgaatc gcacgagatg ccccgtaagc aggcggctgc cctgctgacg
    18601 gacctgactt cgatgatcca gaagcatctc aagaagggcg cccgcgttcg catcggcggg
    18661 ctcggcgtat tgcaggttcg caagcgccct gcccgcatgg gccgcaaccc ggccaccggc
    18721 gagccgatca agatcaaggc gagcaagaag atcgccttcc ggcccgccaa ggaactgaaa
    18781 gagcttatct gacggcgagc tacaggttat ccgagcgcgc tcgggttgaa tacgccccgg
    18841 aaaggaatcc ggggcgtatt cgttttgagc tatcgatacc acttgtacgg gttcttgtag
    18901 cgggaataga agcgtgcatc gacgcaagtc tcgccattcc agtaccggaa agtcccgcac
    18961 gtaccggcag gattaaacgg gggtgcaaag tagccgaccg gcggtggtcc gtagtccttg
    19021 actgatccat acgggtgata cggcctcggc ccggagtaaa cgacgggccc atggaaccct
    19081 acggcggccc gcgtctgcgc tgcccggttg gaatcataga tgacgcgctt gtccctggtg
    19141 aaaatcgcgt cgtaagccac ttcccgctgg cgccagagat caggcggacc gttcacgaag
    19201 gcagcaccgt aaccataacg cggggcgccg ttatagcgaa tcctgacctc cgtgaacgtc
    19261 tggatgatct tctcgccgcc cggggcttcg atcagctccg gctcggagac gtaggcataa
    19321 tagccggcat cctcgggata gccgcccgca gcagcggggg tggcaaatgc gataccggtg
    19381 agagctagtc cgacagcggt gcatttccaa ttcggcatgg gggatctcca ttcagcatcc
    19441 ccccgccgtc aaacgcgtcc gcccacttct ggttcctgaa aaggcggacg gaggagcttt
    19501 ttgatagcgt tcccgaatga ggtggaaaat ggcagcagcg ggaactcgct tcacctctcc
    19561 cgtggggaaa ggtcggcccg aagggccggg tgagggcgta gacaattgcc actggcagaa
    19621 ccccctcacc ccgaccctct tcccgccggg gagagggagt taaactttct agcccgttcg
    19681 agcccggact aattcgtcaa gccgggtcga caccataaat ggatctcgcc gcttccgcgc
    19741 tgatccttcc gtcctcgaga tcgcgcagcg tggccttgcg atcgcgctcg gaaggtgggc
    19801 ccatgcctcc cccgcccggc gttttgatca caaggcgctc accgggggga acgagctgga
    19861 agcctttgcc cttgaggacc ttgccggacg cgagcgcgac ggcaccattt gcaccgttgt
    19921 gtccaccatc acagccgcgc gccggataat cgatgcggtc aaagccggcg agaagctcga
    19981 atggctcccc gatattgctc tcgatttcta tgatctggcc caacccaccg cgggtgcgac
    20041 cggccccgcc ggaatccggg cggagctcct ttttccagaa cagaagcggc gtaatctgct
    20101 cggcaatttc gatcggggtt cctttgactc ctgaggggta ggccgtcgcg gaaaggccat
    20161 ccgattgcgg ccgcgcgccg gtcccgccat tcgacgtcac cgtcatcata aaaccgtaat
    20221 tgccgccctc gccacctttc acgtgcccgc ggacattcag gttccaaagg caggccgcgc
    20281 cttcggccgg aacgcgatcg ggaatggcct gccgcaagca tccaaaaaca acgtcgggca
    20341 gcatctgccc tatcacgtgc cggatgagca caggtgatgg tttgggtgcg ttgaggatcg
    20401 tgtctgccgg agcggaaacg gtgagcggtg ccagcgagcc tgcgttgttc ggcaccttgg
    20461 aagcgacgat gcagcccaat ccgaatgccg tataagccgt ggtgtaggcg agcggtacgt
    20521 tgatgccgcg ccgcgtcggt gcgctgctgc cggtatagtc gacgtgaatc ccggtatcgc
    20581 tgatcgtggt cgtggcggaa aggacgatgg gctcgtcgta gccgtccgtc accatcgtat
    20641 tggaccaggt gcctttcgga agcttggcga tctcggcgag tacggcttcc cgggaacgct
    20701 cgatgatgtg ctcggcaagc tggtcgagat cgtcgagctc gaattcctcc atcatctcga
    20761 tcagccgctg gcaaccaacg tcgttgcagg cggccaatga ataaacgtca ccgacagtat
    20821 cgatgggcag ccgggtattc gcccggatca tcgccattaa cgtttcattc acctttcctt
    20881 catcgagcag tttcaggaac ggaatataga gcccctccat gaaaacgtcc gtcgcatccg
    20941 ggccgaaacc gatgccgccg atatccatca agtggctcgt gcaggaaaag agcccgacga
    21001 gcttgccctt gtgaaagcac ggcgtggtga cgacgaagtc gttgagatgt cctgtgccca
    21061 tccaggggtc gttggtgata taggcatcgc cgggcttcat gttttcgagc ccgaaatgag
    21121 cgatgaaatg cttcaccgac tcggccatgg aattgacgtg gcccggggtg cccgtgaccg
    21181 cttgggccag cattctgccc tggcggtcga aaatcccggc cgaaagatcg cccgcttccc
    21241 gcacgatggc agagaaggcc gttctcaaca gcgtctgcgc ctgctcctcc acaacggaga
    21301 tgaggcggtt ccacatgatt tgcagctcga tatcgcccgc cattttttcc gtcatgaccg
    21361 ggcctccgag gtctttgcag taagtacgag cgccatgcct gcatcgacga tggcgtcgaa
    21421 cgtggtggac agatagctcg atgtggcgtc ctcgacgatg acggccgggc cgctgatttg
    21481 cgtccctggc tgaagatttc cgcgctcgaa gacgggcacc tcgatccgtt tgcccaaccg
    21541 ggcgtcaaag acaactcgtg ttccggtcgg ttgaggcggg ggctgtggca cgggcgggcc
    21601 gagcctggcc gggcggctct ctgcgccgga gacgagcacg acccagctca tgatctcgat
    21661 ggccgcgccc ggaatgtgcc gcgcaaaaag ccgatcgtat tccgcctcga aagcctgacg
    21721 gagggaggcg ggatccgtct cggttaaaga ccggcttgga agctcgaccg ggatttcgtg
    21781 cccttggccg gagtagcgca tgaagacgcg acgttgctct gagagcggcg catcccccgc
    21841 ggcttccctg gcgagggccg tcgcttcggc gctcatggat tcaaggagcg cgttggcggc
    21901 ggcgacgtcg aaccggtcga gacgcatgta gagcgagcgg acgagctcga atgcggccgg
    21961 cgcgtcgaga aagccgatgg ccgagccgac gcccgcaccg gccggaatga tcacgcgctg
    22021 gatattgagc ttttcggcga cccgcgcggc atgcagcggt gctgcacccc cgaacgcgac
    22081 gagcgtgcgc gcccggacct ccgcgccgcg ctcgacggca tggacgcgcg ccgcgttggc
    22141 catgttctcg tcgacgattt cagcgacacc gtatgcggcc gcatcggttt cgagctccaa
    22201 aggctggccg atatgcgtat ccagcgcggc ggccgccttg cgggcatcga tctcgatttt
    22261 gccgccggca aagcgagcag gatcgatctt gccgagagcg acgtcggcat cggtgacggt
    22321 cggctcggtg ccgccgcgat tatagcaggc aggtccaggt tccgagcccg cgctgtccgg
    22381 cccgacgttg atacgcttga gggcgtcgac ccgggcgatc gaacctccgc cggccccgat
    22441 ctcaaccatt tcgatcaccg ggatgcgcaa cgggaagccg ctgcccttca tgaagcgcga
    22501 tgagcggtcc acctcgaaaa cgcgcgcctt gaagggtcga ccctcgtcga taaggcagat
    22561 cttagcggtc gttccgccca tgtcgaagga aagaatgcgg tcctcgccgc gctctgcggc
    22621 tatgcgcgct gcaaggatgg cgccgccggc cggacccgac tcgatgagcc gaaccgggaa
    22681 gttcaccgcg gttttcaggc tcgcgaggct gcccccggag gtcatcaggt gaatcggagc
    22741 cgtgcagccc ctcgatctga actcctgttc cagcctgccg agatagcccg ccatcagcgg
    22801 ctgcacgtag gcattcgcta cggctgtcga tgtccgctcg tactcgcgag cttcggggca
    22861 gacctccgag gagagcgtga tgctgaggcc cgggcaatgc tcgtgcagaa ttgcgcggat
    22921 tcgctcctcg tgcatcgggt tgacgtagga atggatcagc gcgacggcga cggcctcgat
    22981 ctcctcggcc ttgaggcggt ttgcaacccg gacgacggcg gcttcgtcga gcggcagttt
    23041 caccgtgcca tggacgtcga ggcgttcggg cactgtgaag cgcaaacggc gcgggaccaa
    23101 cggctgaggc ttctggatga agacgtcata ctgatcgaat cggctctcat cggcgatctc
    23161 cagcacgtcg cgaaagccct cggtggcgat cagcgccgtg cgcgcgccct tgcgctcgat
    23221 gacggcgttc gtggccagcg tggtgccgtg cacgaagagg cgaatgtccg cgaacgtctt
    23281 cccggcgctg gcgagaatgg ccgccgtgcc ctcgacgacg gcacgctccg gagccgaagg
    23341 tgtcgtcagc acttttgagg tcaggcgctc ggagccgttg tcgaggacga tgtcggtgaa
    23401 ggtgccgccg atgtccacgg caagtcgcaa ttcgggtgtg actgaaccca tatcgtttcg
    23461 atttccgctg agaggctgat ggcgggccgg actatggcat tggtcggccg ctatttacta
    23521 cgacgaagtt gcagggcgcc tagcatgcga cacaagagcg actcatgaca ttccagtggt
    23581 gttgacgtcc ttccgggccg atcggcgtct agcctttggc ggctcattct aaccggtcgc
    23641 atacccataa gggctggcgg gccgatgtgc aacaagccgg cgaacgatat ggccggcaat
    23701 gcgcgcgcaa gtcgcgcgga ggcgccccct ctgtgggcgc cgcggacatg gcgtctcgtt
    23761 cggctggcgg aaaccggaca acaacaaccg tcttacacga accaaatcgg cgaacgggcg
    23821 ttcttcgcca gtccggcgcg ggggatcagg ttgacactcg tcaaacatac tccggccacg
    23881 tcgcgctatt gagggcagtc cgcgaaaacg aggcttggaa cgatggcaca acgaaaggga
    23941 tcggctgcga cggcgcgcac gccggcgtca gacaccttgg aaccagtcgg cgccttgacg
    24001 ccggtccaca aggcggacat tcagcgcgaa agcatacctc gcaagcccag tccaccgcct
    24061 ccgccgagcg tctacgagac ggatacggcc accatcttcg gcgaggcgat ggacaagtcg
    24121 ctcaactatc ttgtttcgcg cttcaccatg ggcctttcgc cggtggccct gacggaagcc
    24181 tatctcgact ggcttattca tctcggtgct gcacctggca agcgtatgga gcttggtgag
    24241 cgggcaattc gctacatggc ccgtcttgcc cgctacaccg cgacgtgcgc tatgagccac
    24301 caagccgtgc agccctgcat cgaaccgatg ccgaacgaca atcgctttcg aagagaagaa
    24361 tggcagttat ggcccttcaa tgtcatccac caggcatttc tgctcaacca gcaatggtgg
    24421 cacgatgcga caacgggcgt gcggggggtg acgaagcagc acgagcgcga gctggagttc
    24481 gcatcgcgtc agttgctgga catcttctcc ccgtcgaatt tcctctggac gaatccgacg
    24541 gtgctgcaag aaactgcatc agagggcgga ctcaacctcg ttcgaggtta tctcaacttc
    24601 atcgacgact ggcagcgcac gctgaaccac cgccggcctc ccggcatgga ggagttcaaa
    24661 gtcggcgaga cgctcgctgt cacccccggc aaggtcgtct atcggaaccg cttgatcgag
    24721 ctgatccagt acgagcccgc gacggatcaa gtgcggcccg agccgatcct catcgtcccg
    24781 gcctggatca tgaagtacta catcctcgat ctgcggccgg gtaagtcgct ggtcgagttt
    24841 ttgacgaagc aaggcttcac ggtcttcatg atttcgtgga agaacccgac ccgcgaggat
    24901 cgcgacttgc gcctggagga ttaccgccgg ctgggcgttc tggcatccat cgaagccgtc
    24961 cagaagatcg tgcccggcca agacatccac ggcgtcggct attgcctcgg aggcacattg
    25021 ctcgccattg cggccgcggc actcgcccga accagaaggg atccgttcaa aaccctgacc
    25081 ttcttcgccg cacaggtcga tttcgaggag gccggcgagc tcatgctgtt catcaacgag
    25141 agccagctcg tcttcctcga cgacatgatg gaaatgcagg gcttcctcgg caattaccag
    25201 atggccggcg cattccagct gctccgctcc aacgatctca tctggtcccg catcatcaac
    25261 gagtatttga tgggcgaacg tgagccgatg ttcgacctga tggcctggaa tgccgatacg
    25321 acccgcatgc cgtatttcat gcattccgag tacctgcgcc ggctctatct gaacaatgag
    25381 ctcgccaccg gtgcctttgt cgtagacgac aggcccgtgg cgctcaccga catccgtaag
    25441 cccgtattcg ccgttggcac cgaaaccgac catgtggcac cctggcggtc cgtctacaaa
    25501 atgcacctgc tgctggacac cgagttgacg tttctgttga cgaacggcgg gcacaatgcc
    25561 ggagttgttt cggagccagg tcaccctcgc cgggcctatc gggtccacac gaggaaggag
    25621 gatgacctct atgtcgaccc cgatacgtgg ttcaatgaaa cgcccgttaa gcggggttcc
    25681 tggtggcctg aatgggccgc ttggctagcg gagcgctccg gcgactggac ctcgccaccg
    25741 cggatgggcg cgcctgagag cggcttcagg ccactcgtcg acgctccggg gagctacgtg
    25801 ctgcagcttt gattgcgaga gggtccgtgc catgcgcgcg atggtgctcg agcagccgcg
    25861 gacggagctg aagctgctcg atctgcccgt tccggaaccc gggccgggtg aaatcgtcgt
    25921 gcgggtcgct gcctgcggcg tttgccgcac cgaccttcac gttgtcgacg gcgagctgcc
    25981 cgacccgaag ctgccgatca tccccggcca cgaaatcgtg ggatacgtaa ccaagctcgg
    26041 gccgggggtc accgcattcg agaccggcga ccgggtcggc attccctggc tcgggcggac
    26101 gtgcgggacg tgccgctatt gcctggctgg acgcgagaac ttgtgcgagc ggcccggctt
    26161 caccggctat acgatcgacg gtggttatgc cgagtacgct gcggcccggg cggattattg
    26221 ctttccattg ccatcggaat tcgaggatgc atccgcggcg ccgttgctct gcgcgggcct
    26281 gatcggctat cggtccctga agctaggcgg accagggcgg gagctcggga tcttcggctt
    26341 cggtgccgct gcccacatcg tcgcccaggt cgcacgctat gagggccgcc gcgtctacgc
    26401 cttcacgcgc cccggtgatg cggcagccca ggagttcgcc cgctcgctcg gggcggagtg
    26461 ggctggaggc tcggacgaga ccccgccgtg cccgctggac gttgcaatca tcttcgcgcc
    26521 ggtcggtgca ctcgtgccgg ccgcgctcaa aggcatggcg cgcggtggca cggtggtctg
    26581 cggcggcatc cacatgagtg acattccttc gttcccttac gagctgctgt ggcacgagcg
    26641 ggtggtgcgt tcggtcgcca acctcgagcg ggaagacgcg agggagtttc tggcccttgc
    26701 ccccaaggtg ccggtcgaaa ccacgatcga gacctttccg ctggaggcgg cgaacgacgc
    26761 tctggcgcgc ctgcgcgagg gccggctgac cggggcggcg gtgctcatcc cctgagcgca
    26821 tgtctcaacc acggccagct atcacgcgtg tcttgattcg actgccgggc ctctcacatc
    26881 tgcgtcagat gcaccgcgta accgaccgga gtgagtagat gctcaccatc gaggacatgg
    26941 tcgcgttctc cgggctgagc cgtgatgaga ttctcgcaat cgccgagcac gagcacctgc
    27001 cggaagccgc ggcgtgcgcc ctggccgagt atctcatgca gcataaacat ggctgcgagc
    27061 gcatcaggga aatgatcgtc gaggacattc gcgccgcgca ggagcggggc gaccgggagc
    27121 acgtgaaaac gcttcttcac gtcctgcacc acttcgtgcg caaccatccc gaatcgctgc
    27181 cgatcggccg cggcgagacc gacgtcacct gatgggccga ggctagttcc ggcttgcgta
    27241 ccaggcgacc ccgagcactg cgacgacgaa gaggatagag ataacgtagc cgggagcact
    27301 gctcatgtgg tcattctgac cgcgcatgca gcaatttttt gaccatccgg cttggatacc
    27361 tcgagcgtat aagccttcag ccgccgatca gcggaagaat gatggcggcc gagattgcga
    27421 tcgcagcgcc cacgacaaat tggctcgcca cgactatggc gacgatctgc atgacgactc
    27481 actccgaatg ggctgatgga ctgcccgttt ctggcttcat ctgcggtttt gatgatcccg
    27541 ccgagaaaag tagcgcccga tccttgagtt gagctggcga gcccgcgtca cggcggctgc
    27601 gacggctcca ttttgcgaca ttgccgtctc gatcacgtct gcatcccgat catccagccg
    27661 gccggcggcg tagtcgacga tcatgtcggg ggtgatctgg cagggaaccg gggtcatagc
    27721 cactccaggg aaactcaatg acgggtcggg tcgccggtca gcggcccggc tgctcgaatg
    27781 ctggagattg cttgccaaat cctgaacggc gccgctgttc cccgcgtaac agacgtcggc
    27841 ccgcccttct tgctcgcatg ctaaacttcg aacatggttt gttgccaagc tcaggcgacg
    27901 aaggagaggc taattctcgg gcttgcaagt gctctgcgcg ccgatcacgg acagtgcctc
    27961 acgatcttga tgcggccagt ccgggatgac ggtgatgagg attgctgagc gacctgagat
    28021 atctggcaca gaaagggttg acgatgctca gagtcttcct ggcgaccacg aacatcttca
    28081 cgtcgctcgt actcggcgcg ctcgccatgg gcgctgtctg gtacttctct ccggacctga
    28141 tgcagagctt ctttcagtcg gccagcggcc tcaagtcatg gctgaccaac ctcggcatcg
    28201 agccgaagta caacaacttc ctctggtttc tgatcgagga gcggcaactc gtgttcatgg
    28261 gcttcgtgat cgcgacccga gtagcaatgg cgattctggc cgcaatcttc attgcgccgt
    28321 tcacggaggc cgtctgacgt cgggtcgcct cagtcctccg atccgaaacg gtgcagatcc
    28381 aggccataga cgcgcagcca ctgcttggcc tcgtccattc ttggcatggt cttgcggacc
    28441 aaagcccaaa agcgcgggcc gtggttcatc tccgcgaggt gcgccacttc gtgtgcagcg
    28501 acgtagtcga ggatcagtgg tggcgccagc accagtcgcc atgagaacga cagcatgccg
    28561 gtcgtcgagc aggatcccca gcggctcacc tggtcacgta cggcgatgcc gcgtggtctg
    28621 aggttgagat ttcgggagtg ccaggcgact ctgtcatcga ggtccgatct ggcttgagcg
    28681 atcagccagt cttgcaggcg ccgcggggca aacgctggat cgccggaaac cacgagctca
    28741 cgcctgtttt cctcgttttc tgcgatggac acgaccgggc cgcgctgcag gcgaccgata
    28801 aaatggatcg tgtgcggtat gccccgcagt ggaatttcgg cgccatgggc gaacgggaca
    28861 ggggtcggca agctcccgag ccgcgccttc acccaatcga tatgtcgtga gacgaattgg
    28921 ccggcctgtt cgaggtcgca ctgcatcggc agcgtcacga tgacggtgcg gcgggtccgg
    28981 ctcacgcgca aggtcatccg gcgcgcggcg ggatgatgtc tgacttcgac agtcgccgga
    29041 atgccctgaa ggtgaagctc cttcgctgct cgcttgccag tcgagcgggc cgcttttctc
    29101 atttagtccg acctcggtgt tgctcctgta agcgatctaa caagtcggag agacacagta
    29161 ccaacgagaa acgcgtagcc cacagcaacc aaaggccgca gtgtggcgat cacgcgccac
    29221 ttaagaagta cggaagctgc aagccgctaa gagcgctctt ttggctcgcc tgaactcaag
    29281 gggaagttcc agcggagcag gttctctagg aaaagaccac ctgcgagcgc catgatccga
    29341 tatggcgctg caaacattcc gctcgcagaa ttcgtggtgt cgtagcgtgg cgcgatgctg
    29401 cccagacctt gagctttgag cttgctcacc atcggatcgg gcgtgcaatc gtttgcgggt
    29461 gagaacgtga aggcgatcga ggccagctca ttctgatagc cgccgcgttc cgcagcagcg
    29521 ctcgcattgg cgtaggccag ctcgtcgagg gtgcggctgc gctgatcatg ggtgcggata
    29581 aattttgcga ttcgcggcgc gatctcgctg cggaaccgcg aaccgttgaa gatgccgtag
    29641 tgcccgacct tcggacagac gaaatgctcc ttgctgtcgc ccgggatgtt gacgcacagg
    29701 tcgtgtgcgg cagcgcattg tccgaccccg gtgatgtcgt ccttctcgcc ctcgatggtc
    29761 atcagcgcga cgcgccggat ggccgctggg tcgatcctgc gtccgcgatg gaccatctcg
    29821 cccttgggca acatgtgccg cacgaacacc gaatcgacgg tctgcagata gaactccgcc
    29881 gtcaaatcca tcacggcaag gtattcgtca tagaactcgc ggtttttctc cgcagactcg
    29941 ccatctcccc gcacgaggtg gttgaacaga tccttgtgcg ccttcaggtg ccggtcgaga
    30001 ttcatggtca tgaatcccgt cagctgcagg aacccgggat agacctcacg tccgaagccc
    30061 agccgtggcc aggggacggt ggtgatgacg ttccttcgga accagtccgt gccgcgctcc
    30121 acggccagtt tgttcacgac ggtcgggctc acacgcgtgt cgatcggccc cccggcgagg
    30181 atcatgctga gcggaacgga ggggtcgtcg tcctcctcca taagcgccac tgccgcgagc
    30241 accggtaccg atggctggca gactgcgaac acatgcacgt cgccaccgaa gaagtggaac
    30301 atgtcgcgca tgtagtcgat gtaatcgtcg agatcgaacc ggccggcgac gagcggcacg
    30361 tcgcgcgcat cctgccagtc cgtgatgtag acctcgtggt ttggcagaag ggtctggacg
    30421 gtgccgcgca gcagcgtcgc gaagtggccg gacatggggg cgaccagcag gacccgcggg
    30481 tcttggacgc gacgctccag gggcaggtcg cgcgcgaagt gcaccagccg acaaaaagga
    30541 cgttgccaga cgacgttctc ggtcacggca acaggctgtc cgtcgacggt cgtggagctg
    30601 atccggaaat cgggtttgtg atagcggcgc gtcgtgcgct cgaacacctc gcaggccgcg
    30661 gcagcgtggc ggccccagcc ggtgtgtgtt agtgggttga gtggattgtt gaagaacagc
    30721 cggcccgcat ccgctgcaac ccgagctggc cgcacagcga cgtgcgtcag ctcgtaccaa
    30781 tggtaaagca tgccccggca cccccgattt atctgtccct cgttatcttt ggcccggccc
    30841 gcttctatcg acgcgctcag gcggaaagtc gcacggtccc tccgcactct tgaccagtgt
    30901 gcaccgattc gggcgcggaa ggaagatcgg gagcagttac aacacttttt tgagtttttc
    30961 taccatggtt gcgacggggg tcgcatgcgt gaaatgcgta acgaactcgc cttgagggtt
    31021 catcagataa atgatcgagg tgtgatcgac cgtatactcg gccgtcgact tctcgtcctt
    31081 caccttgcgg taatagacgc ggtactcctt cgcgacggcc tggatctcct cgggcgtgcc
    31141 cgtcaatccg atcaggcgcg gatggaagct cgacacgtac tgtgcaagct gctcgggcgt
    31201 gtctcgctcc ggatccacgg tgatgaaaat cggcgtgatc ttgtcggctt tgggcccgag
    31261 ctcgtcgagc gctgccgata tgacctgcag gccggacggg cacacgtccg ggcagaacgt
    31321 gaagccgaag tagaccagca tgtacttgcc gcggaaatcc ttgtcggtga cccggcggcc
    31381 ggtgtgatcg gtcagagtga acggcccgcc gacggcagcc ttcccgatcg acgtcacctg
    31441 agacggcgct tgaaggctgt cgagtgcgcc ggggacgaca gcaaggccca ctgcggcacc
    31501 tccgacgaac cctgcagcga taagagccag cagacgaagc cgcatatcga ccagtgctcc
    31561 tgtttccaat cagttgcggg ccgatcccga ctgcatcatt cgccgagttg cacgagggcg
    31621 ccattccgtc gtgcgctctc gagcaatgca cggaagagga tcagcgcacc tgaaacatag
    31681 acgatgttga gaagaagggc gcgcaacata tagtcgccat ggaacgtctg ctgcagaacc
    31741 agcccgcgca gtccttcgaa cacgtatgtc ggtggcagcg cgagcgccag cggctgcagc
    31801 cactcgggca gcgtcgaaac cgggtagtag acgcacgaga tcggcagcag gatgaacacg
    31861 atcagccagg caatgctttc cgcacccaag ccccagcgca gcacggcacc ggtcgagacg
    31921 agtcccagcg cccagctcat gatgatcaga ttggcgaaga aggccgcgaa cgcgatgccg
    31981 aggcccagca ggttgaagcc gaagaagatg taggcgagga tcgcgacggg caccatcgcc
    32041 acggtgagct tcatcaggct caccagcatc agggacgcga tcagctcgat gggcctcagc
    32101 gggctcatca tcaaatgccc gagattgcgc gaccagatct cctccaggaa cgccacagaa
    32161 aacccgagct ggctgcgcac cagaatatcc cagagcagga ctccgccgac gaacaggccc
    32221 gcggcttgcg cggcgaggct ggtcgtcttc gctaggtggg tttgcaggaa gccccacatc
    32281 agcatcgata cgagcggcca gaagatcagc tcggcggtgc gcgggccgga cgagcggatc
    32341 aggtaccagt ggcggcgcac catcccgcgt atgcggcggg cagaggccga caagccggtt
    32401 tgaggaagaa gtttcgtcgt cacgcaggat tccttcattc gcgtccgttg agcagctcga
    32461 acgtgtcggg cgcgcgccgc cgtgcgatat cgaggaagac ctcctccagc gtttcgcggc
    32521 cgaagcggcg gatcagctcc gccggggtct cgtcggccag aatgcgcccg cgctcgagca
    32581 tgatgacgcg atcggcgagg cgctccacct cggccatgtt gtgggatgcc agcacgatcg
    32641 ttgccgagcg cttgcggcaa tagtcggcaa tgaccgaccg cacccagtcg gccgtatcgg
    32701 gatcgagaga tgctgtcggc tcatcgagca gcagcagctc gggctcgttc agcagcgatt
    32761 tcgcgaggct gacgcgtgtc ttctgcccag ccgacagctt accgtagagc cggtcgagca
    32821 actcgccgat gcgcagctca tcggcgatct cgcggacccg gcccgcaaca tcggccacac
    32881 cgtagagctg gccgaagatc tcgaggtttt ggcggacggt gagccgcatg gggatctcga
    32941 cgtaggggct ctcgaagttc agccggtgca aagccgcgtg ccgattgcgc accatgtcca
    33001 cgccgaacac gcgcacgtct cccgaggtcg ggacgacgag ccccatcaac atcgaaatcg
    33061 tggtggtctt gcctgcgccg ttgccgccga gcagggccgt gatagagccg gcgctcaggc
    33121 tgaagctgac gttgtcgacc gcgacgactt ccccataaac cttgcgcagg tcgcgcacct
    33181 gcaccggaag caccgtgccg gcgccatcgg ttctagcccc agattgcaac atggtggtat
    33241 ttccggcgcc gattgagcga cgccattgat gatcgggtga cgagtttcga cctatgtggg
    33301 cagatcgctg ccgggacgca acccgttcgg cagccgggga gaggtaaagc tgaactgtga
    33361 ggctaccgtg gcggtaaccc gttggatggg actggaggaa aggctcgagg ccgagcaggt
    33421 cgaaagccgg ctcagacttc ttaccatcgt ccggctgagg tggttcgccg tggtcgggca
    33481 gctcctcgcc gtcagcgtcg tccattttgg cttgggcttc gagttcccgt ttggcatctg
    33541 cctgctgctc atcgccgcct ctgcgtggct caacgtcttt ttgagcgtgc gctacccggc
    33601 gcggcaccgg ctgggttctg gtttcgcaac tgtgctgctg acctacgaca tcctgcagct
    33661 cgtggcattg ctttacctga ccggcggcat cgagaacccg ttcgcagtgc tcatcgtggg
    33721 tccggtgacc gtctcggccg caacgctgcc gccgatcaac acggtcttcc tcggcgggct
    33781 ggcgctcgca tccacggcct tcctgatcat cgaccactgg ccggtaccct ggtatgccac
    33841 tgagctctta accctgccga tggtctataa ggtcggccag ctcgcggccg tggccgccac
    33901 catgattttc ctggcgctgt acgtctggcg gctgtcccgc gaagctcggc agatgtcggc
    33961 ggcgctgacc gcgacagagc tcgtcctcgc ccgcgagcag aagctgcacg ctttggatgg
    34021 tctggccgcc gccgccgcgc acgagctcgg cacgccgctc gcgacgattg ttctggttac
    34081 gagcgagctg cagcgcgaca tcgagccagg cacgccgctc gcggaggact tggccctgct
    34141 gcgcagccag gccgatcgct gccgcgagat cctgcaaaag ctcacccggc agcccagcga
    34201 gcaggacccg cttcacgcaa ctctttcggt gcatcagctc ctcgacgaag cggccgagcc
    34261 gtaccgcaat tcggggaagg ctatcgagat ttcatgcgcc ccggcggagg gggtgagcgg
    34321 cccgcccgcc cgggagccgg tcggcgagcg ccggccgggg ctgatctatg gcgtcggcaa
    34381 cctgatcgag aacgcagccg atttcgcccg cagcaaggtc gagatctcgg cccgatggaa
    34441 cgaggacacg cttgaaatcg aggtcaagga tgacgggcca ggcttcccgc ctgagctttt
    34501 ggaaactttg ggcgaaccct atattacaac acgaccggcc ggatctgggg ggcccggcaa
    34561 gcgggcagcc ggccttgggc tcgggttctt catcgccaag acactcctgg agcgatccgg
    34621 cgccaccctg acgctggaca atcgggcgcc gcccgagcac ggcgctattg ttcggatcag
    34681 ctgggcgcgc gaagtattcg aggcgttcag tccgggaccg ccgggggccg cggtctctta
    34741 ggcgctgctg caactagacg atctttgata agggtcaggg aacatagccc ttggatgcgg
    34801 catttcgaca gctatatatg tgaggtcagg agaacagtac cgtggccgaa gaatttgcat
    34861 tcagtaaaga agagcttccc gaggatactt ccctcgttat cgttgacgac gaccgggcct
    34921 tcctgcaacg ccttggccga gcgatggaaa accgcgggtt cgaggtccgc acggcacagt
    34981 cggtcgccga gggcttggag ctgatccgcc agaaggcacc ggcgttcgca gtcgtggata
    35041 tgcgcctcga agatggcaac ggcctggacg tcatcgccga attggcgcgc atccggccga
    35101 atgctcgcgc catcgtgctc accgggtacg gcaacatcgc gacggcggtg tctgctgtga
    35161 agctcggcgc cgtggattac ctcgccaagc ccgccgatgc ggacgacgtg accgatgctc
    35221 tgctggcacc gcccaacgcg aaggctgctc cgccagagaa ccccatgtcg gcagaccggg
    35281 tgcggtggga gcacatccag cgtgtctatg aattgtgtaa ccgcaacgtt tccgaaaccg
    35341 cgcgtcgttt gaacatgcat cgccggacgc tgcagcggat cctggcgaag cgcgcgccga
    35401 agtaagcgca atcattgttc gaattgggtt ccgaaacgcg tcaggccccg cggagtaggt
    35461 ttccgagtgc cgagcctgga tccgcaattg actggagacg cgcggccgca acacgaccga
    35521 atcgcaggga catgacccgg cggcggtggg ctggcagcct gacttcatcg acggaccgaa
    35581 cgatctcgcc tccgtagcga tctgccacga tcagcccggc cgtttctggt atcaactctt
    35641 gcggaaagga gggcgaaacg gcgaagtaga gcttgtcgca gtactcccag tactccggcc
    35701 acttttggtc cgatcggaag tcctcgacgc tggacttgat ctccacgatc cagaactcgc
    35761 ccttctccga cagaccgacg atatcggcgc gccggccgtt cggcagaacc agctcagaga
    35821 tgctggccag tccaaggctg gcaaggaggc gaccgacgcc gcgcgcgatc tgaagggctg
    35881 tcgcggattg tcgcccgtcg ttgagcggcc ccttgtcgag accgatcgga atcgccatcg
    35941 gttcgccatc tgctggtgac atttccgcaa ctctctcgcg agcggccgga gcgtaacact
    36001 acttttcatc ttcttttcgc cgggcccacc ctgacatacc gtgcaggcca aagggaacat
    36061 cacggggagc gtgtcataat gttcttcaag cgggcgccac aaggcaacaa gtcagctgcg
    36121 gccgaagctc agggttcggc agggcaggtg ccgtcgatgg aggccgctcc ccacccaccg
    36181 ctgccagtca gcgagctgcg gcgcaacgtc gacccggcta gccttggatt cgggacgacc
    36241 gccgagctcg agcccatcac cggattgatc gggcaagaca gggctctgcg cgccatcgag
    36301 ttcggcgcga acatgaggag ttacgacttc aacctgttcg tgctggggcc acccgcctcc
    36361 ggcaagagta ctgccgtccg cgactatctc acaaagaagg cggccgagat gccgccgccg
    36421 cccgactggg tctacgtcaa caatttcgag aatcagaacc gtcctcgcgc tctgcagctg
    36481 ccggctggcc gcggccgcaa gctcgctgag cagatggtgg ccgccatcga cgagctgcgg
    36541 aacaccatcc ccgccgtctt cgaaagcgag gaatatcagg cgcggcgccg cgccatcgaa
    36601 gagcagttcc gctccagcca ggaagaggcc ttcgaggagc tcaaccgcaa ggcccaggcc
    36661 cagaacatcg cgatcctgcg cacgccgatg gggttcgcca tggcccctgt ccacgagggc
    36721 aaggtggtga agcccgaggt cttcaacgcc ttgccggagg acatgcgcaa gaatgttcag
    36781 gccaagatcg aggcacttca gaaagagctc gaggccattc tcgagcgcgt accaaagtcc
    36841 gacaaggagc gccgcaacaa gctgagcgag ctcatcgagg agatggccca aggcgccgtg
    36901 cgcgaggcac ttgacgatgt tcgcgccaat ttctccgact tgccaaaggt tttggaatac
    36961 ctcgacgctg ccggcaagga cctcatccgg cacgtcgggc tgttcgtcgc ccagcccggt
    37021 gacgagaacg agctgatcaa gcaggccatc gatacgtccc gcgacccccg cttccgccgt
    37081 tacatggtca acgtaatcgt cggtaacggc gaatcctttc cgggggcccc tgcgatcgag
    37141 gagatcaacc cgacctacgg caacctgatt ggccggatcg agcacatcgc gcagatgggc
    37201 gctctggtca ccgacttcct gctgatcaag cccggcacgt tgcacaaggc caacggcggc
    37261 tacctgctca tcgacgcgcg caagctgttg ctgtccccgt atgcctggga agccttgaag
    37321 cgaaccatca aggcgcgcga gatccgcatc gagcagccgc tggaaagcct gggtctcatc
    37381 acgacccaga cgctcgatcc ggaacccatt ccgctcgacg tcaaggtcgt gctgttcggg
    37441 gatcgcgagc tctattactt gctcgccgct ttcgatcccg acttccgtgg cctcttcaaa
    37501 gttcaggccg atttcgacga cacgatttcg tggtcggatg agaacaacct cgcctacgcg
    37561 cgcgtcatcg cctcgatcgt ggagaagtac aagctgaagc cagtcgacgc ctcgggcgtg
    37621 gcccgagtga tcgaggaagg cgcgcggctc gccgacgatc gtgaaaagct gtcgatcgag
    37681 atcggccgca tctccgatct gatccgtgag gccgactact ggtcgagcat ggccgcccgc
    37741 gaggtgacca cccgcgagga cgtcgagcgg gccatcaagg aaggcatcga gcgcgccagt
    37801 cgagtccgcg acagggcgca ggagatcatc gagcgcgaca tcgtcttcat cgacaccgac
    37861 ggtgccaagg ttggccagat caacggcctt tccgtcattc aaatcgggac gttctccttc
    37921 ggccggccga gccgcatcac cgcccgggtg cgaatggggc agggccgcgt cgtcgacatc
    37981 gagcgcgagg tgaagctcgg cgggccgctg cactcgaagg gcgtcatgat cctctggggc
    38041 taccttgccg ggcggtatgc cgaggatgta ccattggccc tggcggctac cctggttttc
    38101 gagcagtcct atggcggggt cgacggagac agcgcctcct cgacggagct ctatgcgctg
    38161 ctgtcggcac tctccgaggt gcccgtcaag caaggttttg cggtcactgg atccgtgaac
    38221 cagaaaggcg aggtgcaggc gattggaggg gtcaatgaaa agatcgaagg cttcttcgac
    38281 atctgcaagg cacgtggact caccggcaac cagggcgttc tgatcccgaa agcaaacgtg
    38341 cagcacctga tgttgcgcga ggacgtggtg gaggctgtgc gcgagggcaa gttcgcaatc
    38401 cacgcggtga gccatgtcga cgagggcatt gaaattctga cgggcatcaa ggcgggcgag
    38461 cgcggcgagg atggacagtt tcccccgggc tcggtcaaca ggctggtcga ggacaagctc
    38521 aaggcgtttg ccgaacgtgc gaaggcgttc ggccggtcca ggggcgattc gcaggataac
    38581 gggaagaatt cggcatgacc gcgcacgtcg cggaggcagc agaggcgcgc ggacgcgtcg
    38641 tactccactt gcatacagtc aatccccatg cggtggccat cgaggcggcc gtacgggtgg
    38701 cgcaggcgtt ccagtcaggt atcgagtccc tgttcgtcga ggacgcgcag atcttcgacc
    38761 tggcgagctt tccctttgcg cgggagatct ccttcacggg gcgcagatcg cgggcgattt
    38821 cgtccgacga gctcgagcat gagtttcgcc ttgcgttcgc ctccattcag cgccggatcg
    38881 cggagctggc gcgcgccgcc gaggtgccga tccgccagcg catcgttcgg gacgatccca
    38941 ttgccgcatt ggcgtcgacc tgtgcggagt gcggaccttg gaacgtcgtt gccttggccg
    39001 agccgatcgg cacgatgagc ggcgagtcct tgcgccagct cttcgaggcc gtgtccgatg
    39061 ccacggggct ggtgatcgtc ggcccgcacg cacgccgcac gtctgggcct gtgatcgtcg
    39121 cggtggagga catcgaccgc ctgcccagca tggtgagagc gggtgagcgg ctcgtctccg
    39181 caacaggggg ctctctcgtc attctgctga ttgctgacga cgaggaacgg ctgcactgga
    39241 tggagggtca ggcccgcctt cttctcggcg agcgcaccga tgctcagatt gtccgcgcca
    39301 gctcagcccg gggcgcggcc gaggtcgcgg cggaggcgct gcggcgcctg aagggtggct
    39361 tcgtcatcag ccagttcggc gggctggtcg tgcccgcaga tcgcgatctg cgggcgttgg
    39421 ctgcatcgct ggagtgtccg atcctgctgg tgcgataacg ccgctgccgc cgctcgatgg
    39481 aagcgcgcgc gggcgcgtgc ttgccgccct ggagggaagt gcctacctcc agtccaggta
    39541 cagccccagc atagggtgac ggccatcagc ttccagcagc atatcggttc gcgcgcacgt
    39601 ctgcggtcgc tcgtcgagcg gctttggcga gaggtgacgc cgccgcgggc ggtggctggc
    39661 gttgccgtag cggtcgtgct ggtcctctgg ctcgccggcg gcatggcgac atgggtggcg
    39721 ctcgtcacca tcggcctcat tgctgcggtt gaggtggccg ggtctcgcgg gcttggtcct
    39781 gccccgcggc gcagggaagc cgagcccttg ccagaaggca ccgcagcgtc atctgaaacg
    39841 gccgtcggtg tgcccgatgt cagctggcgt gccgtgatca gtgccatgcc cgacccggcg
    39901 ctggcgctcg atcaggacgg catcatcgtc caccataatg ccggcgtggc cgatcttttc
    39961 ccgcgcgtca gggtgggtca gcccatttcc ggcgtgatcc gcagccctga gctgcttgcc
    40021 gccatcgaga aggaccgcga gccggaggac gtgacagtcg tcgagctgca gaaccgggtt
    40081 cccgtcgaac gtcgggtctc ggcaattgtc acggctcttc cgttgagtgc cgatgcaggg
    40141 cgcaatcgcg ccctgctcgt aacctttcgc gacatcacgg atcaggaaaa gcttgcccag
    40201 atgcgggcgg acttcatcgc ccacgccagc cacgagctgc gaaccccgct cgcctcgctg
    40261 cgcggcttcg tcgagacgct gcaagggccg gcgcgtgacg acccgaaggc gcgcgaccgg
    40321 ttcctcgaaa tcatggcagc ccaggcaacg cgcatgacgc ggctcatcga cgacctgctc
    40381 tccctcagcc ggatcgagat gcacgttcac gtgccaccgc gggggatcgt ggagctgaac
    40441 gaggtcgtcg cgtttgtcgc ccagtcgctc gagccggtcg cggaagccgc ggggatctcc
    40501 atcgcagtgg agccgcttgc cactcctgcc cgcgtgcgcg gcgaccggga ggagttggtt
    40561 cagctcttcc agaatctcat tcacaacgga atccgctatg gcaaggaagg cgggcacgtg
    40621 cgcgtgcgga tcgcccggga gccgctggcc gggggtcgtg ggcagcgtat ttcgttggcg
    40681 gtccaggatg acggcatcgg cattccgccg gagcatcttc cccggctgac cgagcgcttc
    40741 taccgggtga gcgcctccgc aagccgggag aaaggcggaa ccggcttggg cctggcgatc
    40801 gtcaagcaca tcgtcacccg tcaccgcgga gagctccgga ttgcttcgaa gcccggtgaa
    40861 ggggcgacgt tcaccgtcct gctggatgag cttcccgctc cggccgagct atccccgatg
    40921 cgctgattgt tccgcaatat cagacactta aactgtcatg aaagcattgc gactctgcct
    40981 tatcagaggc gccgtgccgc tgtggcgcac atgcggaagc actggatcgg acctgatcga
    41041 cagggcagga ggattggtcg tgaagatttc tgcagcattg aagcttgcgg cggcgggcgt
    41101 cgcgacatgg ggcatgttgg ccggagcagc atgggcggct gacaacatta ccggtgcggg
    41161 cgccagcttt ccctacccgg tctatacgaa atgggccgac gctttccaca aggagacggg
    41221 catcggagtc aactatcagt cgatcggctc cggcggcggc gtcaagcaga tcaaggccaa
    41281 gacggtgaca ttcggcgcca gtgatgcgcc gctgagcggc aaggacctgg aggcgtccgg
    41341 cctcgcccag ttcccgatgg tcatgggggg catcgtgccg gtggtgaaca tcgatggcgt
    41401 ggcacctggg cagctcgtcc tcgacggtcc cacgctggcc aagattttcc tgggcgagat
    41461 caagaggtgg aatgatcccg ccatcacgaa gctcaatgcc gacctgaagc tgccagatcg
    41521 ggccatcgcc gtcgtccatc gctcggacgg ctcaggcacc acctacaact tcgcctacta
    41581 cctggccgag gtcagccctg agtggaagtc ggaggtcggt gtcagcacgg ctctgcagtg
    41641 gcctgtcggc atcggcgcga aaggcaacga gggcgtcgcc aacaacgtcg gccagaccaa
    41701 gaacgcgatc ggctacgtcg agtatgccta tgcgaagcag aacaagctct cgcacgttag
    41761 gatggtcaac aaggccggca aggtggtctc cccggatgcc gagagcttcc aggccgccgc
    41821 tgccaatgcc gactggaagt cggtgcccgg ctacggcgtg attcttgccg atcagcccgg
    41881 cgatcggtcc tggccgatga cagcggcgac ctggatcctg atccacaaaa gcccggccga
    41941 tccggctgct gtggaaaagg cactcaagtt cttcgcctgg gcctacgcga atggcgatac
    42001 agcggccctg gagctcgatt acgtgccgat gcccgacaac gtcgtgagcg aggtgcaagc
    42061 catgtgggcc gccgagatca aggacggcag cggcaaagcc attttcacgc agtgattgaa
    42121 gctggttccg gtcaggtatt gcgtccgggg agctccgagc acgaggctac cgggctcgtt
    42181 ccatgcggtc cggtagccca gtcttgaaag ctaagagggg atagcggtgt gacacaaaca
    42241 gccactgggc ttggacgtca tgacgccgca aagcagcaga tggaacggca gcgtgtcctg
    42301 aaccggctga agctgtggga tgcgctgttt gcgcgcctta cgttcgccgc tgcgctgctc
    42361 gttctcatcg ttctcggcgc ggttttcgtt tcactgatcg agggagcatg gcccgcattc
    42421 cgtacgttcg gcctcaaatt cttcgtgacg gaagcctgga acccggtcac ggaaaaattc
    42481 ggggccgttg cgccgatcta cggcacgctg gtcacgtctt tcattgcgat gctgatcgcg
    42541 gtgccgatcg gattgggcat cgccatcttc ctgaccgagc tttgccctta cgccctgcgg
    42601 cggcctatcg gcattgccat cgagctgctg gctggaatcc cgagcatcat ttacggcatc
    42661 tggggtctgt tcttcttcgc tccattcttc cagcagacag tacagcccgc gttgatttcg
    42721 gcattcagaa atgtaccggt gctgtccgag ctgtttgcag gtccccctta cggcatcggg
    42781 attctgaccg caggaatcat tttggcgatc atggtgctac ccattatcag cgccatcagc
    42841 cgggacgttt tcgagacggt gcccgctgtc ctgaaagaat cggcctatgg cctcggctgc
    42901 acgcgatggg aggtcgtgcg ttccgtcgtg atgccgtaca cgaaggtcgg cctgatcggc
    42961 ggcttcatgc tcggactggg gcgcgcgctc ggcgaaacga tggcggtcac cttcgtggtc
    43021 ggaaatgcgc atcggatctc cgcttcgatc ctggcacccg ggacgaccat ctcggccacg
    43081 atcgccaatg aattcacgga agcggtcagc gaccttcaca cggcttcgct gattgcactc
    43141 ggattgatcc tgttcctcat caccttcact gtgctggcgc tggctcgcct gatgttgctg
    43201 cgcctcgaag taaaggctgg gagatagcgg atgatcttgg atcaggcgcg atttgctcag
    43261 cgcaaacgcc ggagcttcat cgtgatggct ctgtgctggg gagctgccgc ggcaggtctc
    43321 ggcgcgctgc tcgtcatttt gggcacgctg ctgtccaagg ggcttggtgg aattcggccc
    43381 gaagtgttca cgcagatgac gccaccgccg ggaagcgatg gcggattgct caacccgatc
    43441 gtgggcagct tgatcattac gtcccttggc gtcctcatag gcacgccgat cggcattctg
    43501 gccggtacgt acatggccga gtacgggcgc tacagcaagc tgacgtccgt cattcgtttc
    43561 gtcaacgacg tcctcttgag cgcgccgtcc atcgtcatgg gcctcttcgt ctatgcggtc
    43621 atggttgcgc cgatggggca tttctccggc cttgccggct ccgtcgcctt atcgcttctc
    43681 gtcattccga tcgtcatccg gactaccgag gacatgctcc tgctcgtgcc caatgcgctg
    43741 cgtgaagcgg ccaccgcttt ggggatgccg cggtcgctcg tcatccggcg ggttgcctac
    43801 aaggcggcgc gcgctggcat cataacgggc gtgcttctgg ccgtggcgcg tatcagtggt
    43861 gaaacggctc cattgctttt cacagcgctc aacgatcagt tctggagcac taacattttc
    43921 gctccaatgg ccagcctgcc ggtggtgata ttccaattct cgctcagtcc ttatgaagac
    43981 tggcagcgtc tggcctgggt cggagcgttg atcattacct ttgccgtgct cgcgctgagc
    44041 gtggtggcgc ggtggctggg cacttcgagg gtcgcgaaat gaatatcatg ccaaccgtca
    44101 ccgatcaggc tttgaccgag aagatttcgg tgcgaaatct cgatttctat tatggagagt
    44161 cgagggtcct caaatccatc aatttgccgc tctacgaaaa ggaggtgacg gcgttcatcg
    44221 gcccctcggg ttgcgggaaa tcgactctcc tgcgggtcct gaaccgcatg tacgatctct
    44281 atccgaacca gcgggccgag ggcgaggttc tgctcgatgg cgagaatatt ctttcgccga
    44341 agcaggatct caatctgctg cgctcgcgca tcggcatggt cttccagaag ccgacgccgt
    44401 tcccgatgac catatatgag aacatcgcct tcggtgtccg gctttatgag aagctgtcga
    44461 aacgggaaat ggacgagcgt gtcgaaagct cactccgccg cgcggcgctt tgggacgaag
    44521 taaaggataa gctcaacgcc agtggattga gcctgtccgg cggccagcag cagcggcttt
    44581 gcattgcgcg cacggtggcg atcaagccgg acgtcattct tctggacgag ccctgttcgg
    44641 cactcgatcc catttcgacg gcgaaaatcg aggagctgat cgacgagctc aagcgggatt
    44701 acacgatcgt aatcgttacg cacaatatgc agcaggcagc acgcgtctcg gagcataccg
    44761 ccttcatgta cctcggcgag ctcgtcgagt tcgggctgac gagcgaaatt ttcaccaacc
    44821 ccagaaaacg gcaaacgcgg gactacataa ccggccgcta cggttgagat ccggtcgcgt
    44881 ttggagcatt ggaggaaacc atgaaggagc acatcgtccg ctcatacgaa gaagagctgg
    44941 cgctgctcga tcgcaggatc gcgcagatgg gcgggctcgc cgagcatgcg ctgggacacg
    45001 ccttcgatgc gctcgagaag cgcgatccac aactcgccga gtcggtgatc aagtcggata
    45061 aagcgatcga ccagctccag cgcgatatcg aaggccaggt gatcacgatg atcgcacgtc
    45121 ggcagcccat ggccgatgac cttcgtcacg tcgtggcggc gctgcgcatc acctctgatc
    45181 tggagcgcat cggcgatctt tccaagaata cggccaagcg cgcccttgcc gtctcgcacg
    45241 agccctaccc gaagcccatc atgtccggcc ttcgcagcat ggtggagcgt gcgatgcgcc
    45301 agctcaagga cgtgctcgac gcctacgcgg aacgcaatag tgacctcgcg ctcacggtct
    45361 ggcgcgagga tgagcagatc gatgccatgt ataattcggt gtttcgcgag ctcctgacct
    45421 acatgatgga ggatccgcgc aacatcggcc tctgcaccca tcttctgttc ggcgccaaga
    45481 acatcgagcg tatcggcgat cacacgacca acatcgccga gagcgtctac tacctcgtcc
    45541 atggcatctc gatcacggag gatcgcccca aggatgatca gacgagctcg actatgatct
    45601 cgagcgattg acacgtgacc aacaggagct tggatgtccg tcaaagtgct gatcgtcgaa
    45661 gatgaagccc cgttggcaga gctgcttcgg tacaacttcg aggcagaggg tttcgaggtc
    45721 gcccacgctg caacgggcga agaagcggag ctcctgatcg ccgaggagaa gcccgacctc
    45781 atcattctgg attggatgct gccgtcggtt tctggcgtcg agctttgcag acgcgtcagg
    45841 gcgcggcagg acacacgtgc gcttccaatt ctgatgctga ccgcgcgcgg agaggaaagt
    45901 gaccgcatcc gcgggctctc gaccggtgcg gacgactacg tcgtgaagcc attctcgctg
    45961 cccgagttga tggcacgcgt gaaagccatt ctgcgccgga cagcgcccga gcgcatggcc
    46021 gacgtgctca gggcagcgga tattgagctc gaccgtaccg cgcatcgggt gacgcgcggc
    46081 aaccgtgagg tgcggctcgg gccaacggag ttcaatctgc tggagttcat gatgcagagc
    46141 cccggccgcg tgttctcgcg tgagcagctc ctaaatggtg tctgggggcg tgatgcctac
    46201 atcgacgaga gaactgtcga tgtccacatc gggaggttgc ggaaagcgct tgtccgcgga
    46261 aaagaacgcg acccgatccg caccgtgcga ggtgcggggt atgttttcgg cgagcggcga
    46321 acggcttaaa acagagcggc gaaatatcgc aggattgttt ttagcaccgc cacaattgct
    46381 tactttcgat cgaggcgtcg ctgacagatt gggcgagagt caggccgtta acctttggac
    46441 gcttcttttt ttgtgaagat tgtgttagat tacttccgtc gtagcgtagc gaaaacggat
    46501 gaggaacgcg gagcccggct aacggcctcg cgtcataatt gcctggatga cccacgccgc
    46561 gctgttcggt cttgacctga gagactcaat gcgcggcatg aaagccaagt cattgatgct
    46621 gaaaggcagg ctatgcccgg cacgggggcc atagttggtc aaggcacgct cccgccgacg
    46681 gcaactttgt cggcaaattg accttgtcac gaagccctga ctttgggcgg gatgcccggc
    46741 cggcagacga acggcgggct gcgagttcca gagagtgaga taacgaaatg gctcagaaga
    46801 cgaaggtgcc tgtcacggcg aagagcacca ggggaagtgc caagccgaca gaagccaagc
    46861 gtgccactgg ccgggagcct gaaaagaagg ccgcggctgc caagtcttct tccgtgaaag
    46921 ctcgcgctgg aaagcaggaa aaacaggcca aaaccaagag tacgagtgga aaacaattga
    46981 taaaaaaaca ggtagcaaac aaccaaccgg cagcaagcaa gaaaattgcc ccggcgtcta
    47041 aacagcccgc gggtcaggct gccaaagtga gcgcaaagac gtccgccact gtcgagaaga
    47101 caaggccggc tgagaagccc gtcgagaagt cagcgctgcc tccggcagcg atgcgcgttg
    47161 gtcagcccgc tccgacccag cagcggcgtg cgatgacggc cgcgatggcg aaggcggctc
    47221 agcccaagat ctcggctccg gtcacgccaa aggatgttgc tgcgagaacg aaagaggcgc
    47281 ggcaggaggc gcaggccgcc aagaagtccg tcagcgcccg tcacggcttc aagacgaacg
    47341 agtacatcgt ctatccgacc cacggtgtcg gccgcatcgt cggcatcgag gagcaggaaa
    47401 tcgccggcat gtctctcgag ctgttcgtga tcacgttcga gaaggacaaa atgacgttgc
    47461 gcgtgcccac cggcaagctg cagagcgtcg gcatgcgcaa gctggcgtcc gaggacgtcg
    47521 tcaaaaaggc gatggacacc ctcaagggcc gcgccagaat caagcggacg atgtggagcc
    47581 gccgcgcgca ggagtatgag gcgaaaatca actccggcga tctggttgcg attgccgagg
    47641 tcgtccgcga cctttatcgg tccgagtcgc agcctgagca gtcctactcg gaacgccagc
    47701 tctacgaggc tgccctcgac cgcatggcgc gggagatcgc tgccgtcgaa aagctcgatg
    47761 agcaggcggc tgtgcagcgc attacggaag tgctggcgaa gagcgcccgc ggccgccgtg
    47821 cggccgagga agcggaggct gaaggcgacg acgacggcga ggccgatccg gccgtgcgag
    47881 ccgcctgacg ccgtccagct ttcgttatca atcgttgaca gcggtccggt gccgtgcggt
    47941 gccgggccgt tttcattgaa gctgcgcgtt gaccgcggtg ctcagcggct cgctccagcg
    48001 gcaggcccga aacctgcgcc gaaccgtccg cgtcagggct cgacctgaca tctgctatca
    48061 agacatctgc ccatcaagcc acgccgacgc gtagcagatc gtggacgtgc acgatcccga
    48121 cgggcttgcc tttctccacc acgaacaaag cggtgattcg ggcggcgttg atcagctcca
    48181 gggctgctga ggcgagcgtg tcgggggtga cggtcttcgg cttgcgggtc atgatatcgg
    48241 ccgcccgcgc ggacaggagg cgctcgccca tgttgcgccg caagtcgccg tcggtcacga
    48301 tccccacgag cttgccgttg gtgtcgatta ctccgaggca gccgaagctc ttctgcgtca
    48361 tcgtgaccag cgcggcgctc atcgactcgc cttccaaggc aagaggcagc tcttcgccct
    48421 tatgcatgat gtcggcgacg tacttgagct gcgcgccaag cgagccgccg gggtggaaca
    48481 gtttgaagtc gtgtgccgtg aagcccttcg cctccagcag agcgatagcg aggctgtctc
    48541 cgagggcgag ctgcatggtc gtggacgtgg tcggggcaag gccgtgcggg caggcctcct
    48601 tcgcccgcgg cagctcgagc acgacgtcgg acgaacggcc gagcgccgac tcccggcgtg
    48661 aggtgatggc gatcagcggc accttgaagc ggcgcgaata ggtgatgacg ttcttgagct
    48721 ccaccgtctc tccggaccag gagatcgcca ggatgacatc ctccgccgtc accatcccga
    48781 ggtcgccgtg agccgcctcg ctcggatgaa cgaagaacgc cggtgtgccc gtggacgcga
    48841 aggtggccgc gagcttctgg ccgacatggc cgctcttgcc gatgcccgtc acgatcaccc
    48901 gtccgcgtga cgccgagatc acacggattg cttcggcgaa gtcgctcgcc atctcatgct
    48961 tgagggctcc tgccagcgca gaaagcccct ctgcctcgat ctcgacggtg cggatggcgc
    49021 agcgcaccgc ctccgcgcgc ccgctcttca gccggctatc gacgcccgtc ggcttgctga
    49081 catctgtcgt gtgcagccca ttgcggtgcg ctgccggttg agccgtcatt ggacccccct
    49141 gattctcacc gatgacgtgg actcaaaaac gggtgctttt gtgtctaaca gacacctgcg
    49201 tcggcgccaa cgaagccagg cgggtcgaat ccggcctatc aaccggaacg tgagctggca
    49261 tcgaccagac attgagctgt cagtgtgctt tcgctgccgc tgccggcttg ctacagttcc
    49321 cgcccaggga ccctgaaacg gcaggcttga gatgacactc ggcgcccatc ccttcttcca
    49381 gcctgagaaa cgtccgcatg tggaaggcga cttccggcgc gaggaggtgg cgcttgcgaa
    49441 ccgtaattcg ggccttccgc tcgaggcgct gcgccacgac ctgacgccga ttggattgca
    49501 ctatctgctc atccacttcg acatcccgta tgtcgaggca cccgagcgct ggaacgtgaa
    49561 ggtcggaggg cgggtgagca acgcgatcga ggtgccgctt tccgaaatca agcggcttcc
    49621 gcagcgaagc ctgagggtca cgctcgaatg cgctgggaac ggccgggcga agctgacgcc
    49681 gcgctcccaa agtcagccgt gggggaatgg cgctgtcggc acggccgagt ggaccggcac
    49741 cccggtgaga tacctcctcg agcgcgccgg tatggctgct gacgccgtcg atgtcgcctt
    49801 tttcggggcg gatcgcggat tcgatgccgg cgtcgagcac gactacggcc gcagcctgac
    49861 gccggatctt gccatgtccg acgacgtgct tctcgtgcat acgatgaacg gccagccgct
    49921 gctgccgcag catggctttc cgctgcggct catcgtgccc ggctggtatg gcatggcaag
    49981 cgtgaagtgg ctgaaccgga tcgaggttct cgaccggcca ttcgatggct tccagcaggt
    50041 caggacgtat gtctaccgtc aacactcggg cgacgaaggc gttccggtca cgcatatgcg
    50101 cgtgaaatcc ctgatggtgc cgcccggcat tccggactgg tacacgcggg ctaggctcgt
    50161 cgagcgcggg cctgtcgagc tgtacgggcg cgcctggtcg ggcggaggag tgccgattgc
    50221 catggtcgag gtgggcgtgg acggcgcgtg gcaggaggcg acgcttgatc ctccggcggg
    50281 ccagtacgcg tggcgtggct ggcgtttcac gtgggacgcc cagccgggag agcacgagct
    50341 catgtgtcgg gcgacggacg aggcgggcaa cgttcagccg ctcgatgccc cctgggacag
    50401 cggcggattc ggcaataatg tcgtgcaccg cgtgacggtt acggtacgct gatgagctgc
    50461 cggccacgtc agacgtaatc ggaaggggaa ggcatgccat cgagtgcgca atcggcgcag
    50521 cctgccagct cggccatcga ggtgctgatc tggcgcggac gggagaacgg tcactatcag
    50581 cggttcacgg tgcccttgcg cgaaagccag accgtgcttg acgtggtcac ctgggtgcag
    50641 cgcaattccg atccgacgct cgcctaccgc ttcgcctgcc gggtcggcat gtgcgggtcg
    50701 tgtgccatga cggtcaacgg ccgcccgcgg tggacctgcc ggacccacgt cgccaatgtc
    50761 gtcgagtacg gcaggctgga gatcgcgcct ttggcgaacc tgcccatcat caaggacctg
    50821 gcgacggaca tgagccgctt cttcgagaag tggcagcagg cccgcgcgcg tttcgagcca
    50881 tcgcgcacgc ggcacgaacc cgtcgagacg atctcgccaa acaccgccga gcgcatggcg
    50941 gcggatgacg ccatcgagtg catcaactgc gccgtttgtt acgcggcttg cgataccgtg
    51001 cgctggaacg acgattacct tggtccggct gcactcaatc gcgcctggac gctggtcaat
    51061 gacgtgcgtg atgcaggcca gaaggagcgc ctgaaggctg tctcggatgt cggcggttgc
    51121 cagagctgcc actcacatca atcctgcgcg gagctctgcc ccaagtcgct caacccgacc
    51181 gcctcgatcg ccagcctgaa gcggcgtgcc atgtgggcgt atctcaaggg ggagctgtga
    51241 tggtccttga agctattccc gacgcagcag caccctccaa tgtcatcccg ggccttgtgc
    51301 ccgggatcca agtctcgtct agctctgtgg ccctctgtgg aactggactt cgccacgtcg
    51361 ccaccgtcga tggtgcggaa ccgtggatcc cggcgacaag cgttgggatg acggtgggtg
    51421 gcggggcgaa cctctcaaac tcggctgcct ggaggctcgc gtcatgaccc tccgcctgta
    51481 cgttctccag cgcctgaccg cggcattgat ggtcccgctg atcatcggcc acctcatcgt
    51541 cattttctac gccacatcga acgggctctc ggcggccgaa attctcggcc gtacgcgcgg
    51601 gagcattggg tgggcgctgt actacggcct cttcgtcatc ctcgcttccg tccatggcgc
    51661 gatcggcgtc cgcggcgtgc tccgcgaatg gacgaggctc acgccgggcg cgctcgatgc
    51721 catcatgtgg gcgttcggtc tgctcctcgc gattttggga gcgcgtgccg tcgcggccgt
    51781 ggcgctgcca tgacggtcca ggtcgaaaca cggcgaacga cggctcaccg gcgtcaaggg
    51841 ctttggattg ccgccctggt gcatcgcctg tcgggaatcg cgctcgcctg tttcctgccg
    51901 ctgcacttcc tggcgctcgg acttgcgatc gagggcgaaa cgcgtctcga cggcttt
//
LOCUS       DASVDU010000145         4694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1201025, whole genome shotgun sequence.
ACCESSION   DASVDU010000145 DASVDU010000000
VERSION     DASVDU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1201025"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(558..3206)
                     /locus_tag="VF226_09275"
     CDS             complement(558..3206)
                     /locus_tag="VF226_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7074218.1"
                     /translation="MKSRDESQSDDMSRWYAMSRPECLKRYGSRPTGLDSAEVTGLRK
                     RFGENRLPEREGPGWFALLLRQFASPLILLLVAAAAVSMLLGEITDAFFIIAVLLINA
                     IVGAVLEGRAERSIAALNRIVRADAVIWRDGMRRRIDSTELVPGDVVELESGTIVPAD
                     LRLLDSGELAADESLLTGESLPVDKDARATVPIEAVLADRITMAYAGTAILRGRGLGL
                     VTAIGRGTEVGRIAEGLSTHAQAPAPLIVRLERFSRSFGLMVLVAVGVFASAQFLRGM
                     PLADVFFLSVALAVSAIPEGLPIAITVALAVSAHRMAKRNVIVRALPAVEGLGACTVI
                     ATDKTGTLTLNRLTVGAVWLPIHGRLPIHNQADSGSLRSLHQKPANKASDVRKLAMAG
                     VLCNEASLDSGDGKPVGDTVDVAFLLFGAMLGVRRSDLTTEHPIVATIPYESQRRYAA
                     IFLRAGSTVDVVVKGAPETVLPMCAGPRDDAWAAAEQLARDGYRVLAVARGSVAANRS
                     FDETALTGLDLVGLAGLIDPLRPEAEASVRDAGRAGVRVCMVTGDHPATAFAIAREIG
                     IANDPSEVVTGQDLARTAHSGQQALVAHGRVFARVEPLQKLSIVEALRDAGHFVAVTG
                     DGVNDAPALAAAHIGIAMGRTGTDVARRAADLVLADDNFASIVGGVEEGRIAYANIRK
                     VIYFLVTTSAAEIGLFVFCLLWGLPLPLFALQLLWLNLVTDSIQTIALSLERGEPGLL
                     SRGPRLPRERVFDRRMIEQTALAGGIMTVIAVSSFAWLLEHGRSVEEARNLLLLLMVS
                     FQNAHVFNCRSEWRSTFILPLGSNPWIVIVVLAAQFLHIGALYTPGLSDVLHLQPVSL
                     GEWAGVAALSLALIASTEMYKALRRS"
     gene            complement(3601..>4694)
                     /locus_tag="VF226_09280"
     CDS             complement(3601..>4694)
                     /locus_tag="VF226_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7074219.1"
                     /translation="ATPAHSASLWLARLNVPKPAYQEAPQIHEFTGPAGEALRGIVAA
                     IRLPAADRDLVFAVAGPVSEIRRDVVTFAHQLALTLTILTLGLAGAVLAQVRFGLRPL
                     RTIQTALAKIRSGEAGRLPDALPAEVRPLAEELNNLLARNATLLQRARRQAGDLAHAL
                     KNPLTVIRNEAREIESDRGQRLREQVALMSAAIERNLARARAAGAGGVPNARASVERV
                     VDDLCFTLRHLHDDRCLAFQTAGLAELWFAGDVQDLEEMLGNLMDNACKWAHQRVLVR
                     GERAGRRVRIFVEDDGAGIPEVRRAEVLQRGRRLDETTPGTGLGLGIVQDMTELYGGS
                     VTLGRSALGGLQAELDLPAAGLPLARRSH"
BASE COUNT          942 a         1588 c         1398 g          766 t
ORIGIN      
        1 cggccagacc ggacctccga acccggaccg gagcaggttc cgcgcaaccg ccgccccgac
       61 cgagcctttt cggcggctgg cgccaatgag cgcgacgctg gctggccgaa gcaatgcatc
      121 gaggtttcgt atcgtcacgc agaatactcc tgcctggatc gcggaattgg ctggcaacgc
      181 taatcaaccc tatccaatcg cagacgatcg agccttgatt cggatcaacg tgcctaagga
      241 catgccactg cggcggatga actgggtaat tcatgtcaat gccgccaact ttccggtaga
      301 ggaaaaggtc acgggattgg acctactgcg aaagcgcccg gccatgacgc ccaaagccat
      361 agccgtcttg gcgcgatgag tctcggcggt gcggcgcagg acattccggt gcgggcggac
      421 cagccggtgc cgatggcgca gtgacagctt acagaggcat gccggcagga aatcgggatc
      481 ctgtcggtct gatcgaggac ctcttccgag gcaccgccca aaacttacga agcgaccgcg
      541 ctcacggcgc cgccggctca agagcgccgc agcgccttgt acatttccgt cgatgcgatg
      601 agcgcaagcg aaagggccgc caccccggcc cattcgccaa ggctgactgg ctgcagatga
      661 agcacatcgc tcagacctgg cgtgtagagc gcaccgatat gaaggaattg cgccgccagg
      721 acaacaataa cgatccaggg atttgaacca agcggcaaga tgaaggtcga ccgccactcg
      781 gagcgacagt tgaagacatg agcattctgg aaactcacca tcaggagcaa gagcaggttg
      841 cgtgcctcct cgacgctcct tccatgctcc agcagccaag caaacgagga cacggcgatc
      901 accgtcatga tcccgccggc gagcgccgtc tgttcgatca ttctgcggtc aaagaccctc
      961 tcccgcggca gccgcggccc tctcgatagc agccccggct caccccgctc cagtgacaga
     1021 gcgatggtct gaatagaatc cgtcaccaga ttgagccaaa gcaactgcaa tgcgaacagg
     1081 ggcaatggca gcccccagag gaggcaaaaa acgaacagcc cgatctctgc agcgctggtc
     1141 gtgacaagaa aatagatcac cttccgaatg ttggcgtagg cgatgcgccc ctcctcgacc
     1201 ccgccgacga tcgacgcaaa gttgtcatcg gccagaacaa ggtcagcggc gcggcgcgcc
     1261 acatcggtac cggttcggcc cattgcgata ccaatatgcg ctgccgcaag ggccggcgca
     1321 tcgttcacgc cgtcgccggt cactgccacg aaatgcccgg catcccgcag cgcctcgacg
     1381 atgctcagct tctgaagcgg ctcgacgcgg gcgaacacgc ggccatgggc caccaaggcc
     1441 tgctgccccg aatgcgcggt cctcgcgaga tcttgcccgg tcacgacctc cgacggatcg
     1501 ttcgcaatcc cgatttcgcg tgcaatggcg aatgcggtcg cgggatggtc gccggttacc
     1561 atgcagacac ggacaccggc gcgtcctgcg tctcggacgg atgcctctgc ttcggggcgg
     1621 agcggatcga tgagtcccgc gagcccaacg agatcgagcc ctgtcaaggc agtttcgtcg
     1681 aaactacgat tcgccgcgac cgagccgcgt gcaaccgcca acacccgata gccgtcccgg
     1741 gccagttgtt cggccgccgc ccacgcgtca tcccgcggac cagcgcacat cggcaggacg
     1801 gtttccggtg ctcccttcac gacgacatca acggtgcttc ctgcccgaag gaagattgcg
     1861 gcataccgtc gctgggattc gtaggggatc gttgcaacta tcggatgctc ggtagtcagg
     1921 tccgatcgac gcactccgag cattgcaccg aagaggagaa aagcgacatc gacggtatct
     1981 ccgaccggct tgccgtcgcc gctatcgagg ctggcctcgt tgcatagcac ccctgccatg
     2041 gccaactttc gaacgtcgga cgccttgttt gccggcttct ggtgcagcga ccgcaggctt
     2101 ccgctgtcgg cctgattgtg gatgggcagc cggccgtgga tcggcaacca taccgcgccg
     2161 acggtcagtc ggttcagggt caacgtacct gtcttgtccg tcgcaatgac cgtgcatgca
     2221 cccaacccct caacggccgg gagcgcgcgc acgatgacat tacgctttgc catgcgatga
     2281 gccgatactg cgagagccac cgtgatcgcg atcggcaggc cttccggtat cgccgaaacc
     2341 gccagggcaa ccgacaaaaa gaaaacgtcc gcgagcggca tgccgcggag gaactgcgcc
     2401 gacgcaaata ccccgacggc aaccaacacc ataagcccga acgacctgga gaagcgctcc
     2461 agtcgaacga taagtggtgc cggtgcttgc gcatgcgtcg agagaccttc ggcgattcgg
     2521 ccgacctcgg tgccccggcc gatcgccgtc acaagaccga gcccccgccc tcgcaggatc
     2581 gccgtcccgg cgtatgccat cgtgatccga tcggccaaaa cggcttcgat cggaacagtt
     2641 gcgcgggcat ccttatccac gggtagcgac tcgccggtca gcagcgactc gtcggccgcg
     2701 agttcgccgc tgtcgaggag ccggagatcg gcgggcacaa tggtgccgga ttcgagttcg
     2761 accacatctc cgggaacgag ttcggtcgaa tcgatacggc gacgcatacc gtcacgccag
     2821 atgacagcat cggcgcgcac tattcggttc agcgcggcaa tgctgcgctc cgccctgcct
     2881 tcgagcaccg cgccgacgat ggcgttgatt agaagcacgg cgataataaa gaacgcgtcc
     2941 gtgatctctc ccagcagcat cgacacagcg gcagccgcga cgagcaggag aatgagagga
     3001 ctggcgaatt ggcgcagaag cagagcgaac cagcccggac cctctctttc cgggaggcgg
     3061 ttctctccga accgcttccg gagaccggtg acttctgccg aatcaagtcc ggtgggccgg
     3121 ctgccgtacc gcttcagaca ttccggcctg ctcatcgcat accatcgcga catgtcgtcc
     3181 gactgagatt catcccgtga tttcactcgc cggcctccct cccgtttcga tcacagcatg
     3241 cctatccttt cggggcggaa tacgagtcgt ctcaatgccc cttcttatgt gcgggatggc
     3301 tgccacgtct ccccgccgag tcggaagact gcttcgatcc ggatgcgatc atgtgcgcca
     3361 gcagcgggac ggcacctgaa cccaagctta aatcggcctt cagcgcggcc tcagcagtaa
     3421 tcgaataggc tttcctcgat gacagaaacc ggccgccgta cgagagcacc ggtggcatcc
     3481 acgcgcggcg acactcccca atgcatgtcc cacggtagtg gtcaccccac tcctgcgggt
     3541 gatcaacgcc gctaatgttc tcgccgcacc gcaggaagca caggccgaaa cggcggcaac
     3601 tcaatggctg cgcctcgcga gcggtagacc ggctgccggc aaatcgagtt ccgcctgcaa
     3661 gccgcccagg gccgaccggc ccaaggtgac ggaaccgcca tagagctcgg tcatgtcttg
     3721 aacgatgccg aggccgagac cggtacccgg cgtggtttca tccagccgtc gcccgcgctg
     3781 gagcacctcc gcgcgccgca cctcgggaat acccgcacca tcgtcctcga caaagatccg
     3841 cacccgcctt cctgcccgtt ctcctcgaac gagaacccgc tggtgtgccc atttgcaggc
     3901 attgtccatc aggttcccga gcatctcctc aagatcctgg acgtctcccg caaaccacag
     3961 ctccgccagc cccgccgtct ggaaggcgag gcaacgatcg tcatgcaaat gcctcagggt
     4021 gaagcaaaga tcgtcgacca cgcgctccac cgatgcccgc gcattgggaa ccccgccggc
     4081 gccggccgca cgggcgcgcg ctaggttacg ctcgatcgcc gcgctcatga gcgcaacctg
     4141 ctctcgcagt cgctgcccgc gatcgctctc gatttccctt gcctcgttgc gaatcaccgt
     4201 cagagggttc ttcagcgcgt gcgcgaggtc gcctgcctga cggcgcgccc gctgcagcag
     4261 ggtcgcattg cgtgcgagca ggttgttcaa ctcctcggcc aacggcctca cctccgccgg
     4321 aagcgcgtcc ggcagacgcc cggcctcgcc cgagcggatc ttagccaggg ccgtctgtat
     4381 ggtacgcaat ggccgcagcc cgaagcggac ctgagccaac accgccccgg cgagaccgag
     4441 ggtcagaatg gtcaaggtca atgccagctg gtgggcgaaa gtaaccacgt cgcggcggat
     4501 ctccgaaacc ggccctgcaa cggcgaacac gagatcccga tccgccgcgg gaaggcggat
     4561 cgccgcgacg atcccccgca gcgcctcgcc tgccggcccg gtaaattcgt gaatctgcgg
     4621 cgcttcctga tatgcgggct tagggacatt caagcgagca agccatagag aggccgagtg
     4681 cgccggagtc gcct
//
LOCUS       DASVDU010000146         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_275154, whole genome shotgun sequence.
ACCESSION   DASVDU010000146 DASVDU010000000
VERSION     DASVDU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275154"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..680
                     /locus_tag="VF226_09285"
     CDS             54..680
                     /locus_tag="VF226_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074220.1"
                     /translation="MISDEYLAARRIQSGDDNADIETAQFRRDLFIELIGDHPIDTYT
                     AADLQAYVLLLKYWPAKVKDRPEGLTAREIIETNQDLSRKPLSIKTLREGYVANIKAM
                     MRSQMMEYDYRDPFAGARIFYPEIASPSQPTEPVNGGAKSGHAAAQNQARWQWPAPWR
                     ARLPIAGACHGALASLGQNERSDLRTFVAAFGQCCWRGDSSRRSSRGC"
     gene            complement(604..1368)
                     /gene="istB"
                     /locus_tag="VF226_09290"
     CDS             complement(604..1368)
                     /gene="istB"
                     /locus_tag="VF226_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036759113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7074221.1"
                     /translation="MSDGPEILLAHHLKALKLPTFLREHQKLARQCAAEGMDHVRYLA
                     RLVELELIDRERRMVERRIKAAKFPTVKSLDSFDFSAIPKLNKMQVLELARCEWIERR
                     ENVIALGPSGTGKTHIALGLGLAACQKGMSVSFTTAAALVNELMEARDERRLLRFQKQ
                     MAAVKLLIVDELGFVPLSKTGAELLFELISHRYERGSTLITSNLPFDEWTETFGSERL
                     TGALLDRLTHHVSILEMNGESYRLANSTARKRQQKS"
     gene            complement(1365..2861)
                     /gene="istA"
                     /locus_tag="VF226_09295"
     CDS             complement(1365..2861)
                     /gene="istA"
                     /locus_tag="VF226_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7074222.1"
                     /translation="MELYLRVRQACAGGMSQRQAAKAFNISRDTVAKMMTFSVPPGYR
                     RTAEVRRPKLDPFIPIIEGWLEAERSMPRKQRHTAKRVFDRLRDECGFTGGYTIIKDY
                     IRERERRGQEVFVPLAHPPGHAQADFGEAMVKIGGVEQKAHFFVLDLPHSDACYVRAY
                     PAAVAEAWVDGHIHAFAFFGAVPQSIVYDNDRCLVAKILPDGTRKRAVLFSGFLSHYL
                     IRDHYGRPGKGNDKGNVEGLVGYCRRNFMVPLPEFTSWEAFNLWLEEQCRKRQHDVLR
                     GESETIGERLQRDLVAMRPLPVAPFDACDQDSGRVSSQCLVRYKTNDYSVPVAFGHQD
                     VWIRAYVHEVVIGCRGEVIARHPRCWDREEIVFNPIHYLPLLEQKINALDQAAPLQGW
                     ELPEEFATLRRLMEARMNRHGRREYVQVLRLLETFDLADLHAAVKQALQMGAIGFDAV
                     KHLLLCRVEHRPPRLDLDCYPYLPKATVEKTRPGSYMRLLSGDAEDAA"
BASE COUNT          603 a          990 c          973 g          583 t
ORIGIN      
        1 tgaaggaccg ccggtatgta gaacgcccag cctcttcgaa gccgctcttc agcatgattt
       61 ccgacgagta tctggcggca aggcggatcc agtctggtga tgacaatgcc gacattgaga
      121 cagcgcagtt cagacgcgac cttttcatcg agctgatcgg agaccaccca atcgatacgt
      181 ataccgccgc cgatcttcag gcctatgtgt tgctcctaaa gtattggccc gcgaaggtga
      241 aagatcgccc cgagggcctg acagcccgcg agatcatcga gacgaaccaa gacctgtcgc
      301 ggaagccgct gagcatcaag acgctgcgcg aaggctatgt cgccaacatc aaggccatga
      361 tgcgctcgca gatgatggag tacgactacc gtgacccgtt cgcgggggca cgcatcttct
      421 atccggagat agcatcaccc agccagccaa ccgagcctgt caacggcggt gcaaaatccg
      481 gccacgcggc ggcgcaaaac caggccaggt ggcagtggcc tgcgccatgg cgcgcgcgct
      541 taccaatagc tggcgcgtgc catggcgcat tggcctcttt gggccaaaac gagcgatctg
      601 atctcaggac ttttgttgcc gctttcgggc agtgctgttg gcgaggcgat agctctcgcc
      661 gttcatctcg aggatgctga cgtggtgggt cagccggtcg agcagtgcgc cggtgaggcg
      721 ctcggagccg aaggtctcgg tccattcatc gaagggcaga ttgctggtga tcagggtcga
      781 gccgcgctcg tagcggtgcg agatcagctc gaacagcaac tcggcgccgg tcttggacag
      841 cggcacaaag cccagttcgt cgacgatcag caacttgacg gcagccatct gcttctgaaa
      901 gcgaagcagg cgacgttcgt cgcgggcctc catcagttcg ttgaccaggg ctgcggcggt
      961 ggtgaagctg accgacatgc ccttctgaca agccgccagc ccgaggccaa gggcaatgtg
     1021 cgtcttgccc gtgccgcttg ggccaagggc aatgacgttc tcgcgccgct cgatccattc
     1081 gcagcgggcc agttccagca cctgcatctt gttgagcttc gggatggcgc tgaagtcgaa
     1141 gctgtcgagg cttttgaccg tggggaattt ggcggccttg atgcggcgct cgaccatcct
     1201 ccgctcccgg tcgatcaact ccagttcaac aagccgggcg aggtatcgga catgatccat
     1261 gccttcggcc gcacattgcc gggccagctt ctgatgttcc cgcaggaacg tgggcagctt
     1321 cagcgccttg aggtgatggg cgagcaggat ttccggacca tcgctcatgc cgcgtcctcc
     1381 gcatcgcccg acaacaaccg catataggag ccgggcctgg ttttctcgac tgttgccttg
     1441 ggtagatagg gataacagtc caggtccagc cggggcggac ggtgttcaac acggcaaagc
     1501 agcaggtgct tcacggcgtc gaagccgatt gcgcccatct gaagcgcctg cttcaccgcg
     1561 gcatgcagat cagccagatc aaaggtctcg agcaaccgca ggacctggac gtattcgcgt
     1621 cggccatgtc ggttcatgcg agcttccatt agccggcgca acgttgcgaa ctcctcggga
     1681 agctcccagc cttgcaatgg tgcggcctga tcgagtgcgt tgatcttctg ctccagcagc
     1741 ggcaggtagt ggatcgggtt gaagacgatc tcctcgcgat cccagcagcg gggatggcgg
     1801 gcaatgacct cgccccggca gccgatcacc acctcgtgga cataggcccg gatccagaca
     1861 tcctgatggc cgaaggcaac cggcaccgag tagtcgttgg tcttgtagcg caccagacac
     1921 tgggacgaga cgcggccact gtcctggtca caggcgtcga aaggcgcgac cgggaggggg
     1981 cgcatggcaa ccagatcacg ttgcaaccgc tcgccgatcg tctcgctctc accgcgcagg
     2041 acatcgtgct ggcgcttgcg gcattgctcc tccagccaaa ggttgaatgc ctcccagctc
     2101 gtgaactcgg gaagcggcac catgaagttg cggcggcaat agcccaccaa gccctcgaca
     2161 ttccccttgt cattgccttt gccaggacgg ccgtagtggt cccggatcag gtaatgcgac
     2221 aggaagccgc tgaacaggac ggcccgcttg cgggtgccat cgggcaggat cttcgccacc
     2281 aggcaccggt cattgtcata gacgatcgac tgcggcactg ccccaaagaa ggcaaaggcg
     2341 tggatgtggc cgtccaccca agcctcggcc acagccgccg gataggcgcg gacgtaacag
     2401 gcgtcgctgt gcggcagatc caaaacaaag aagtgggcct tctgctcgac accgccaatc
     2461 ttgaccatcg cctcgccgaa gtcggcctgc gcatggcccg ggggatgcgc cagcggcacg
     2521 aacacttcct ggccccgccg ttctcgctcc cggatgtaat ccttgatgat cgtgtagccg
     2581 ccggtgaacc cgcactcgtc acgaagccgg tcgaacaccc gcttcgccgt gtgccgctgc
     2641 ttccgcggca tggagcgttc cgcctcaagc cagccctcga tgatcgggat aaagggatca
     2701 agcttcggcc gcctgacttc ggccgtccgc cggtagcccg gcggcaccga gaacgtcatc
     2761 atcttcgcaa ccgtatcacg ggagatgttg aaggccttcg ccgcctgacg ctggctcatg
     2821 ccccccgcgc aggcctggcg aaccctgaga tatagttcca cgctgaaaat cctcgcgccc
     2881 tccctgctgt cgcagaaagg gaaagggtgg acgactttta cgccgcccgc agcagctcca
     2941 tgccgccgct tccgtggact agtttctcac cgccgttctc accttgacgg ttggctgaga
     3001 ggccgtcgcc gttccggtcg tgagaggtct caattgttat ggcatgctgc ctgcctgctc
     3061 tggtccaagg ggctcatgga gcgcgttcgc ggactggagc atggcgagac ggtggtcatc
     3121 agcattggcg gaaagcccgg caggcaccg
//
LOCUS       DASVDU010000147        34176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_763085, whole genome shotgun sequence.
ACCESSION   DASVDU010000147 DASVDU010000000
VERSION     DASVDU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 34176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34176
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763085"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VF226_09300"
     CDS             <1..591
                     /locus_tag="VF226_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7074223.1"
                     /translation="DGKPAGVIAVADPIKPTTPAAIAELKRLGLRVVMLTGDNATTAH
                     AVARKLGIDDVAAEVLPEDKSKVVERLRAEGRIVAMAGDGINDAPALAASNVGIAMGT
                     GTDVAMESAGVTLVKGDLTGIVRARHLSAATMRNIRQNLFFAFVYNALGVPVAAGILY
                     PAFGILLSPIIAAAAMSLSSVSVIANSLRLSRVDLN"
     gene            complement(569..979)
                     /locus_tag="VF226_09305"
     CDS             complement(569..979)
                     /locus_tag="VF226_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7074224.1"
                     /translation="MEDCLSNLRVLLVEDEPLLAWELELALAEAGATVVGPASTLKMG
                     LALAQQNAIAAAIIDFRLGSEEASPLAALLYERGIPFIIHTGHSTSGHSAWGSPPVVR
                     KPASPDRVVRTLAQFLGEGEGYGATPALSSGRLG"
     gene            complement(1182..4832)
                     /locus_tag="VF226_09310"
     CDS             complement(1182..4832)
                     /locus_tag="VF226_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7074225.1"
                     /translation="MARFKTRPVDGRAWSLSTHLLVLCLSILVPGIILGGLALWNLFL
                     AESASTKMRLLGDASYIAAGIDREILGFTRVGESLASSPLLQEQKFEAFDLQARQLFG
                     LHGWYVILTDRTGKQLVNTTFPSGSTLPTTDLSRIEDVMETGKPQISDLFFSASNIPL
                     VGVRIPVISGGETAYVLTVAVPTQIFTPILDRNIGGLNWTVTLADRKGRIIARSQGHA
                     SSVGKSLSGSTLDQLRLGMPIHRVVSNGTEVVRVVDRSALAGWIVSATVPTADLAAIA
                     RRGWVTFAALAAALTILSVVLAYLFARRIAIPIQTLAGVPLDSSTSRFPETGLAEANR
                     VGSALFESVRQLEASRQQYRTLVEAANDIIFTTDLDGRFLTCNAAGYSALGYSGNELI
                     GRSFNSLLSDKSAHAACEGLPVSTGELPASEDQLQRRFEVEVVARSGRTLVWEVSSQL
                     LRDASGKPESVLSIARDITERRKAEEQIRANEERLRLALAGIGAGVWEYEFASRASNW
                     SPEMLELYGLDQKDFPLSREALLNLVHPDDRERVREITRLHAKKGGPFACEFRVCRPD
                     GRLVWISSRGVIECAADGQPVRIRGIDQDVTAARIAEQHRSELLRTTAQQLNELQSLY
                     NSATVGLALLDREFRFQRINQVLAAMNGYSVEEHLGRVAWDLIPDLRPKVEPILKRIL
                     ETGEPVTGVEVTGETPARPGDQRSWLLQFYPLKSRDGSVVGIGTICEDVTERRRAQLG
                     QAHLAAIVESSSDAIISWSADGRLRSWNPGAERMFGYTADEAIGQGTNLLFTPGPGQS
                     TPRAFELAKSGKRVQFEGLRRRKDGTEIPVSISASPMRDDNGRIAAVSVIYRDISEQK
                     RREEHTRFIMRELSHRSKNLLAVIQAMARQTARTSRDLMDFQDRFTARVRGLAQSHDL
                     LVKRDWRGVPVAELVRAHLGPFVDRSEQGLILSGPSLSLKPEAAQNVGLALHELATNA
                     SKHGALSSPTGCIEIRWHTEQRDDGEHRFHMLWRETGGPEVVQPRVRGFGHTVIDAMV
                     GRALEGEAHLEWRREGLVWRLDAAASCIAPEMSVADAADLLPDGVAGKLNDLDHKWHE
                     LREKSGGLPPPSAIDIAAHPESDAMFLAALGPNPDLKPVKYLHIGETLRRRLGWRGEA
                     EITKRGGDEILDSLEDCRRSTIASRRPCYEWARINVGGEPLEFECLLLPFSEDRKTAS
                     HMLGIVAFKTAD"
     gene            5075..5626
                     /locus_tag="VF226_09315"
     CDS             5075..5626
                     /locus_tag="VF226_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074226.1"
                     /translation="MVYGSESVNEAAGKETGDIGAKIEELRAEMMRLSEQVQVYLQNR
                     AGDLKEDATETSAEVQKLIRENPFPAVGVALGIGFLLGLMMRGGASAAPRLSRRDFDR
                     LASKVRGAIEAGSARAQAAASEVGDTALLERLAGALSSVIGSSRSAAESVGSAGGRAA
                     KSVAAVGERTARNIAERLSQAAH"
     gene            complement(5701..6411)
                     /locus_tag="VF226_09320"
     CDS             complement(5701..6411)
                     /locus_tag="VF226_09320"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HEX7074227.1"
                     /translation="MKAVLVSSFAELYDAVTPLSQPAVFRGDVDAAQALVPHLGRTDC
                     DRETEIQLFREWQRQAVQFLDLPADDWDCLTIARHYGLATRLLDWTINPLVAAFFAAA
                     SSRPGSAALWVFIPGRIVTEADADPFSFTGVALYRPRTLAARISRQEAVFTVHGPPSE
                     PVSESHGELYRVDIDSSFRPVLLKDLARFGISHASMFPGLEGLSRAINWQYGLDRVRT
                     SEPHVEAVNALSNPRFAR"
     gene            complement(6539..9127)
                     /locus_tag="VF226_09325"
     CDS             complement(6539..9127)
                     /locus_tag="VF226_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7074228.1"
                     /translation="MTEVLSLHVSRTTRYLRDRFGPVDLLDNLPVGICCCDAEGVLVQ
                     FNKHAAELWGRSPQVGAQRFTGAERIFDPDGRQLPLEAIPIARVLQTGKPVRNATVMV
                     DRPDGTRVSLIVNAEPLRDDDKIVGAVSCFQDVTNLSRKDEPPNSGNEVLEDFFEHAP
                     IGLHVVAGDGTILRANKAELELLGYSAEEYVGRPVADFHADEHVVEEILARLSRDEVL
                     DRYPARMRAKDGSIRHVLLTSNAEVLDGKLVKTRCFVLDDTGRKLAEARERITETRAR
                     AALASVPVAVYMTDAEGRIIFFNEAYERLIGRKPEPDVDRFCSAWRIYTPEGEPIPRE
                     DCLMAVALKEQRPISGMEVIIERPDGTRVSVLPHPTPISGGDGSLIGAVNVMVDITDR
                     HETDAQLARLAAIVASSDDAIISKSLDGRITSWNAGATRIFGYEEQEVVGKPITLIVP
                     PELHDQELAMLEKLRRGERVDHFETVRVAKDGRRVDISLTVSPIRDRLGRVIGASKVS
                     RDITERKRHEELQKLLIGELNHRVKNTLATVQAIATQTVRSANSPAEFMSGFSGRLQS
                     LARAHSALTRGNWQGVDVEDLLRDQLLLGGAEDEQIAYSGPQVVLEPQVALHLALVLH
                     ELGTNARKYGALSVPTGRLMLRWAVRGGCDEKLLLSWQERGGPPVKPPRQRGFGSMLI
                     EQSLEAQGGRVSVAYPVEGVTCEISLPLPDSRRPAISAGRVTSDSARPTASLGDSLAR
                     SAPGKNPRVLIVEDEPLVSMDVAMALSDAGCGIVGPAANLDKARELIAGDQFEVAILD
                     ANLSGEPVDELAAELTRRGIPFAFLTGYGLEGIPREFRTAPLIDKPFNPGQLVAVVEA
                     LIAGKA"
     gene            complement(9372..9446)
                     /locus_tag="VF226_09330"
     tRNA            complement(9372..9446)
                     /locus_tag="VF226_09330"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9412..9414),aa:Gln,seq:ttg)
     gene            complement(9583..10011)
                     /gene="rnk"
                     /locus_tag="VF226_09335"
     CDS             complement(9583..10011)
                     /gene="rnk"
                     /locus_tag="VF226_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HEX7074229.1"
                     /translation="MKDRHYPEMPEIVVSQADQQRLTVLAMDALNRSPDVAEELIAEM
                     ERARVVPDVPSSVVRMGSVVTYTADDGRERRVQLVFPGQADIGAGKISILTPIGTALI
                     GLSEGQSIAWAARDGKERRLTVVKVENPPDGDGSDGVQGA"
     gene            complement(10305..10766)
                     /locus_tag="VF226_09340"
     CDS             complement(10305..10766)
                     /locus_tag="VF226_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEX7074230.1"
                     /translation="MPKPGDTIIRVLQIRLEPPELGTVTIRMRLESGALELRVEAQRA
                     ATAELIRRDQGTLSRLLQAAGYDVDGLTIHIAEPDRAALANIIHAQPTGSQSTPNLSP
                     HGQLAGSQGDGRPGGESRNPQNGRAGEWTGTAEESPELPHAHSSRPNGVYV"
     gene            complement(11561..12706)
                     /locus_tag="VF226_09345"
     CDS             complement(11561..12706)
                     /locus_tag="VF226_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotC"
                     /protein_id="HEX7074231.1"
                     /translation="MAALLAVTTLSRGEELAEEPYRLVRELTRLQDQVAAGRRKAYPA
                     QHDLTAALSAKLAAAAPKSWGDARNRRAAVLFVLSGGAPAILRTLLSQGRFPDAETEL
                     AAGVLAYAEGRSLEARAHLAGIDARSLDPSLAGAVALVQSSLAAESDHPARVLGLLDQ
                     ARLLSPGTLVEESALRRQIFLLLEAKEPERANFLLRRYIRRFARSDYAIAFWDQFAAK
                     LAEQPTSENSKHLAELESFLKKADAEVQGWIYLSLARQGIARGNRFLVQFAVPRAALW
                     QPNAVRASLYEAAALVLTDDYDRAVSMLMNMNARQFTNEDAGLREAALSVARQIRRLP
                     SDDGQASTPIGEAESPARELSQIGRGTIERAAAMIADVDALLNEGPQ"
     gene            complement(12733..13959)
                     /locus_tag="VF226_09350"
     CDS             complement(12733..13959)
                     /locus_tag="VF226_09350"
                     /inference="COORDINATES: protein motif:HMM:NF004651.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotB family protein"
                     /protein_id="HEX7074232.1"
                     /translation="MNSDFVPRNPNELSQELLIIRRRSGGKHEHPHGGIWKIAYADFM
                     TAMMAFFLVMWLLNATDKQTISAVASYFNPVKLTDHVRKPKGLNDIEADAHQAQDPAV
                     EATRIKGNPTGEVAPEGSFRPTFREETLFSDPYKVLAQLAERAKRDEPDVKRNKKEAA
                     DVAFRDPFDPDFRFLVARANAGELKPAPQPLEESGTDQSFAEPLEHSLEPPSDPSIAP
                     KSRLGSGEAIERTSAEPPKEPGAAPSAGPKEPNPPPPVEKDIAEAIRSSVNNAIPGRK
                     PNIEVVSTSDGVLISLTDEFDFGMFAIASAEPSPELVVIMEQLAKVLKDYPGPLIVRG
                     HTDGRPFRTKTYDNWRLSSARAHMAYFMLVRGGLDESRFERIEGYADRNLKVPSDPEA
                     AQNRRIEILLRTAGTS"
     gene            complement(13956..15593)
                     /gene="fliF"
                     /locus_tag="VF226_09355"
     CDS             complement(13956..15593)
                     /gene="fliF"
                     /locus_tag="VF226_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEX7074233.1"
                     /translation="MILMIGREQIEQLVQNILALGKRRLAALAVIGITAFGAIGLIGY
                     FLSRPDLETLYSGLSAQDVSRIGAVLNEAGIFFDVNAEGSKILVRREQTAHARMLLAE
                     KGLPSSANAGYELFDNLGSIGLTSFMQEITRVRALEGEIARTIQAVKGVKAARVHIVL
                     PDAGSFRRNRQEPSASVVIRTETAGDFASAPAIRHLVAAAVPGLTVDQVTVLSTDGTI
                     LAAAGEASTALPARIVELEKTISKELQERARKTLSPYLGIENFEVSVAARLNTDKRQI
                     NETAFDPDSRVERSVRTVRESGSSQNQSSRSPVSVEQNLPEEDLTTRSGDVSTRSNER
                     REELTNYELNSKTVSTVSDGYRIEALTVAVVVNRKRLLSAIGEEADTAAIEAHLSEIE
                     KLVSSAVGLVPDRGDRITVAAVDFFKDGEALEPVGMGIGELLLSQAGTVVNAFVALGA
                     TALLVWFGLRPAMRMILEAGSAAGAPSAAGRLEAGAGGEPATGEPTLPAAEPNLIADL
                     KSRLSRSPQKRLEQLVDFDEDQAAAILKQWMREARQT"
     gene            complement(15821..17122)
                     /locus_tag="VF226_09360"
     CDS             complement(15821..17122)
                     /locus_tag="VF226_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017275275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEX7074234.1"
                     /translation="MTSLLTNTSAMTALQTLTQTNKNMAVTQNRISTGYRVATASDNA
                     AYWSIATTMRSDNKALSTVQDALGLGAAQVDIAYTAMESAKDIIDEIKVKLVAAKQPS
                     VDKTKIQSEIDQLQDQLVGIASAAVFSGSNWLSVDSSAAGWNAIQKVVSSFSRTGSGT
                     IKIGTIDVDLTKTALFDANATGAGLLDAGQALTADGGLQDTVTPVAGDGSGTPASVTL
                     FTDVDTDGVTLDENDAIKFDLVFNGEATTITIDKRTVDAALGSSDGVVADSTDMASVV
                     ALALQNAGFDTTSDVTVAADGSGGVAITHVSDATDATLAVLNSATSDNGNFFGVSDID
                     ISNATDAQLDAYIAGLDAMAANVTTAASDLGAVKSRIDLQKDFVDSLMDAIDRGIGQL
                     VDADMNEESTRLQALQVQQQLGIQALSIANANSQNILALFR"
     gene            complement(17292..18026)
                     /gene="fliP"
                     /locus_tag="VF226_09365"
     CDS             complement(17292..18026)
                     /gene="fliP"
                     /locus_tag="VF226_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133990.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX7074235.1"
                     /translation="MSRFVGLTALLLILGTAPAGAQTLGLDAVVPPGEGTATGQIFLL
                     VIIITVLSVAPGLLIMVTCFTRFVIALSFLRTGLGLQTTPANLILISLALFMTFYVMA
                     PTFDQAWHNGLKPLVENKIGEEEAYRRITEPFRTFMKANVREKDLSLFEELAGVETRS
                     PDEPIELRVLIPAFMISELRRAFEIGFLIVLPFLVIDMIVATLTMSMGMMMLPPTVIS
                     LPFKILFFILIDGWNLLVGGLIRSFA"
     gene            complement(18023..18538)
                     /locus_tag="VF226_09370"
     CDS             complement(18023..18538)
                     /locus_tag="VF226_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX7074236.1"
                     /translation="MVEIARVFPQRGRKRERRTGPAAVIAAWIGTTLLAVLAGGIFGL
                     QIETIAEQPKREQDEVNTSRPVTPAPAYSESGRIRALPPVVTKLAGERDAWVRIEASI
                     VLAGSPAEEEEIMAARMAEDMAAFLQTVSVSDIEGPSGFLQLREDLAERARIRSDGRV
                     RDFIVHTFIVE"
     gene            complement(18559..19275)
                     /gene="flgH"
                     /locus_tag="VF226_09375"
     CDS             complement(18559..19275)
                     /gene="flgH"
                     /locus_tag="VF226_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX7074237.1"
                     /translation="MRSLAAVAFIGMILSACAVDPADIGREPHLTPVGSGLRPDIVPV
                     LREPEPFPRFRRDNSLWDDSSADLFGDPRARTIGDVVTVKIAIKDKASLDNRSNRKRD
                     SNKKLGLLGSYGAGSNGSSSEWEGELDAELDSVSTSKGEGAIRRSESIQLRVAAVVSD
                     VLPNGNLVISGSQEVRVNYEVRVLNVAGVIRPRDIETDNTIAYDKIAEARISYGGRGR
                     ITEVQQPGVGQQILDLLIPF"
     gene            complement(19272..20057)
                     /locus_tag="VF226_09380"
     CDS             complement(19272..20057)
                     /locus_tag="VF226_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HEX7074238.1"
                     /translation="MPRKRSATHGGLAALALLCLGMAPALAQEAWRPKIPTAEAAPVD
                     NEAELEQGWFPVIINPPKPVVINSSKSAEVSRPSSPTKSRPEQQESTQEQPARKLVHQ
                     SEAPSQEAAEKRPEGHPARSALAEEYCANIADAAADARFAWQKRTLSEMDAELQRRIT
                     VLEKRTAELREWLARRDAFVKKAQETLVSIYERMRPDAAASQLSAMDEETAAAVLTKI
                     KPRLASAILNEMAPGVAARLTMTIAGAAKVNTSKDTERTGDKS"
     gene            complement(20058..21158)
                     /gene="flgI"
                     /locus_tag="VF226_09385"
     CDS             complement(20058..21158)
                     /gene="flgI"
                     /locus_tag="VF226_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX7074239.1"
                     /translation="MRRAGLVVLLILIGLADAAAASRIKDIVSVQGVRSNQIIGYGLV
                     VGLSGTGDSLRNSPFTEQSLQAMLDRMGINIRSAKARTRNVAAVIVTAELPAFAGKGA
                     RIDVTVSSLGDATSLAGGSLVLTPLKGPDGAIYAVAQGPIAVTGFASTGNAEKLTQGV
                     PTTGRIPNGAIVEREAPGTLAGMGILALELHSPDFGTAVRIADTINAYTGQKYGVVTA
                     RADDLRSVKLLKPRNVSAARFIAEIGDLLTETDAPARVVIDERTGTIVIGRDVQISTV
                     AIAHGNLTVRVTESERVSQPLPFSEGKTVTTPETLISAEQEGGHMAIVGGTNLQSLVN
                     GLNKIGLKPAGIIAILQAIKTAGALQAELVVQ"
     gene            complement(21155..21619)
                     /gene="flgA"
                     /locus_tag="VF226_09390"
     CDS             complement(21155..21619)
                     /gene="flgA"
                     /locus_tag="VF226_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX7074240.1"
                     /translation="MALLRSAFNVLIFTGVLLRASPVDAAGISLPVPKITLYPGDFIR
                     TDHLVERTFNVRLKKDSPVHRKRDALIGKVVRRTLLPGKPIPINAVREPHLVVQGYTV
                     QIVFQDGPLVITGYAVALQSGGNGDVVRVRNVDSGVIVSGAVQPDGTVRVGP"
     gene            complement(21661..22449)
                     /gene="flgG"
                     /locus_tag="VF226_09395"
     CDS             complement(21661..22449)
                     /gene="flgG"
                     /locus_tag="VF226_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX7074241.1"
                     /translation="MRALAIAATGMTAQQLNLEVIANNIANINTTGFKRARAEFTDLL
                     YQAERAAGAPIRGGENAVPEGAQLGLGVRAAAIRKLHIQGSLTNTTNKLDLALNGRGW
                     FQVASADGEQLYTRAGAFNKNADGQIVTIDGYVVQPAITIPDDATNIVVNESGEVFVR
                     IENEPELQLLGQFTLANFANEAGLEPLGGNLYRETEASGAPVIGVPGDIGFAKVQQGY
                     LENSNVDPVKEITELISAQRAYEMNSKVIQAADDMLGTITKGIR"
     gene            complement(22506..22829)
                     /locus_tag="VF226_09400"
     CDS             complement(22506..22829)
                     /locus_tag="VF226_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX7074242.1"
                     /translation="MIDQIAPFVSQGAGSPLAVSTATRAPHLASPAHSQAADFGTVLG
                     KLITDSADVLRAAESNAVRGIMGEASVQEVVESVLAAEQTLQAAIAIRDKVTAAYLEL
                     SHMAI"
     gene            complement(22829..23248)
                     /gene="flgC"
                     /locus_tag="VF226_09405"
     CDS             complement(22829..23248)
                     /gene="flgC"
                     /locus_tag="VF226_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX7074243.1"
                     /translation="MIDPLQVSLKIAASGLEAQSKRVRIVSENLANAQSTGNTPGADP
                     YTRKTISFETEVDRITGANLVRVESIDFDRSPYRIEHDPGHPAADASGYVKLPNVNML
                     VEMADMREANRSYEANLQVIKQAREMLSMTIDLLRNS"
     gene            complement(23262..23642)
                     /gene="flgB"
                     /locus_tag="VF226_09410"
     CDS             complement(23262..23642)
                     /gene="flgB"
                     /locus_tag="VF226_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX7074244.1"
                     /translation="MGPVYLFDLISQHNKWLSVRQTTVAGNIANANTPGFKAVDTEPF
                     EAVLRRAPLELAVTQPGHMSPPSAGIPVADVSDEDAWEVTHSGNTVVLEQELIKAGEV
                     NRSFALNAGIIKAFHRMMLASAKG"
     gene            complement(23745..24374)
                     /gene="msrA"
                     /locus_tag="VF226_09415"
     CDS             complement(23745..24374)
                     /gene="msrA"
                     /locus_tag="VF226_09415"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7074245.1"
                     /translation="MIHLITRAVGALLFLATAAAAQTAQAPAESGQKLEIATFASGCF
                     WCTESDFDKVKGVVETTPGYMGGTTPNPTYQQVSSGGTGHAEVVQVKYDPTHVTYKQL
                     LDVYWHNVDPLDKGGQFCDRGDQYRPAIFYHNEEQKRLAEESKAALEKSGRFSQPIVV
                     EITAASTFTPAEDYHQDYYRKNPLRYFIYRSGCGRDARLEALWGKAKTH"
     gene            complement(24406..24909)
                     /gene="msrB"
                     /locus_tag="VF226_09420"
     CDS             complement(24406..24909)
                     /gene="msrB"
                     /locus_tag="VF226_09420"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7074246.1"
                     /translation="MSRRQFGMIGAAAALLGGFTATRGFSAGKSEQETFEITKTDEEW
                     KKILTPEQYHVLREHGTEYAFSSPLDKNYAPGTYHCAGCDLPLFSSETKFDSGTGWPS
                     FWKPIDGAIGTSVDRKFFMTRTEVHCSRCGGHLGHVFDDGPPPTGLRYCMNGVALKFV
                     PKAETSS"
     gene            complement(25009..25773)
                     /gene="sfsA"
                     /locus_tag="VF226_09425"
     CDS             complement(25009..25773)
                     /gene="sfsA"
                     /locus_tag="VF226_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEX7074247.1"
                     /translation="MRFSAPLIRGRLVQRYKRFLADVVLDSGETVTATCPNTGSMIGL
                     TEPGSVVWLSESDSPTRKYRHTWEMVEADLGQGPTLVGINTSRPNALVAEAIGEGLVA
                     GLDGYPDLRREVKYGQNSRIDILLDCAKKGRCYIEIKNVHMMRRAGLAEFPDSVTQRG
                     AKHLLELADMVRSGHRAVMMFLIQRGDALQLDLARDIDPAYGEAFDEARAAGVEMLAY
                     RCRLSPEEIVVDTAVPIEGIEVGGARRRGPKVRTRV"
     gene            25858..26610
                     /locus_tag="VF226_09430"
     CDS             25858..26610
                     /locus_tag="VF226_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX7074248.1"
                     /translation="MALVQDNDIVTAAVLVIGDEILSGRTKDKNIGYIAEHCTAIGIQ
                     VREVRIVPDVEDEIVAAVNALRARYDYVFTTGGIGPTHDDITADAIAKAFGVKIDVDP
                     RALALLLPYYERRGVEVTPGRLRMARIPEGADLIENSISVAPGFMLGNVITLAGIPNV
                     MQVMLDAVTPRLRTGRKMHSVSIDLQAPEGTIAGLFGEHQKAYPSVSMGSYPTFKDNR
                     PSTQLVLRSTDPDLLAEAADGLRQKLAEHGFL"
     gene            complement(26634..27860)
                     /locus_tag="VF226_09435"
     CDS             complement(26634..27860)
                     /locus_tag="VF226_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX7074249.1"
                     /translation="MPAIDPDRVMGDLRRFAEFGKYKTGVHRPTYSREDMAARHWLAG
                     RMADAGLEPEIDGIGNVIGRGKGSGPRILTGSHTESQNYAGWLDGPLGLIYGLEAARV
                     IGADPDFAGKVVDVGAWADEEGHFGNLIGSRSFCGDLTEQEIDEAFDPTRGVKLRDAL
                     ESAGLAGRPRIVCDPKDYIAYVEAHIEQGDVLESSDLNLGIVTSIVGIWQYRVTFEGV
                     TNHAGTTRMAIRKDAGVALTRLCCEIERRLPEVAGPRSVWTTGRITLHPGAASIIPGR
                     AEMLFQIRDADTAILERMERALFDLVSASDAAGPCRASVEALRKGTPAVMDARVQSAL
                     EKAAERIAPGKHMRMPSGASHDAQVFARHMPAGMLFVPSIGGISHHWAEDTKEEDIIT
                     GAQVFTEAVAELLRTA"
     gene            complement(28364..31045)
                     /locus_tag="VF226_09440"
     CDS             complement(28364..31045)
                     /locus_tag="VF226_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEX7074250.1"
                     /translation="MTIRNLQHLLAPKSVAFIGASPQPGSVGLIVTQNLRAGGFDGPI
                     WLVNPHRSDVEGVPCYAKIEDLPDTPDLAVIATPPPTVPGIIDELGRKGTRAAVVITA
                     GVRDELRQAMLNAAKPYLLRVQGPNCIGLMVPGIGLNASFSHRAPLAGDLAFLSQSGA
                     LATAVIDWTIDRQIGFSHVVSLGDMADVDFGDMLDYLAGDPKSRAILLYMESVTHAPK
                     FLSAARRAARSKPVIVVKAGRHEAGSKAALSHTGALTGSDRAYDAAFRRAGVLRVLAL
                     YELFGAAESLARLPASTGERLAILTNGGGVAVLAVDRLMDLQGTLAELSDAGKAALDA
                     VLPPTWSKGNPVDIIGDAGPERYTAALEVLLEEKDTDAVLIMNCPTAAASSTAVAEGI
                     VNLIEKRRQAKQPLKPIFTNWLGEGASREARQLFAEHQIASFEFPNTSIEGFMQLVRY
                     TRAQAQLMRTPPSLPEELGLRTHEAETIIADALRQERHVLSEVEGKSLLAAYGIPTVP
                     TEVARDPEEVAAIASRILQEHAACVVKVLSDDISHKSDVGGVRLGLERATAARDAAQD
                     ILARVARQLPDARVQGFTVQPMIRRPRAHELIAGMAVDRTFGPVLLFGAGGTSVEVVN
                     DTAQALPPLDMNLARDLMERTRIFRLLRGYRDRPAADLDRIAESLVRLSYLVARHPEI
                     REIDINPLLADESGVIALDARVKVEDERKNPRVPMALRPYPSEWVKGIELKGIGEIRL
                     RPIRPEDEPLYADFFPRLTPEDQRLRFFTARPDLSRKFLARLTQIDYAREMAFVALSK
                     KTGELLGVSRFIADPDYTKGEYAVLVRSDLKGRGLGWELMQHLIAYARAEGLEQLFGS
                     VLAENTTMLKMVAELGFSIEPEKDDPTVRHVVLHLRR"
     gene            complement(31078..32658)
                     /locus_tag="VF226_09445"
     CDS             complement(31078..32658)
                     /locus_tag="VF226_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7074251.1"
                     /translation="MAARIGVADTDQSEVARFLADPATYGGSAAAPVERIETHGAMVF
                     LAGDQVWKIKRAVRFPYMDLSTLEKRRATCEREVEVNRIWAPEIYLGCIPITREPDGR
                     LALGGTGEPVEWAVRMRRFPQSDLLGSIADREGISQGLARALADAAFESHEKAPRVND
                     PLGAARLASLVDSVSGNLAGVANVIGQDEATRFRKRAEAQLARAATVLDRRAESGFVR
                     RCHADLHLGNIVMWKGRPTLFDAIEFDEELATIDTLYDLAFLLMDLDFRGLRRAANTV
                     LNRYLWRSQAMLDLDGLAALPLFLGLRAGIRALVAAERAEQEKASGRKAQSVERARRY
                     IEAALSHLDPAPPKIVAVGGLSGTGKSTLASALAPGIGPCPGAVHLRSDLERKALFGR
                     AETERLGAEGYTEEVTARVYATLVEKTGRVLAVGHGVIVDAVFARADERLRLEAEAEK
                     AGLRLKGLWLTASPEVLMARVAARRNDASDATVDVVESQISYDIGLLSPSWTVVDAGG
                     DREETVRRAQKALSGQGE"
     gene            32906..33739
                     /locus_tag="VF226_09450"
     CDS             32906..33739
                     /locus_tag="VF226_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7074252.1"
                     /translation="MAYKTILMHLNHDRRAKQLLGAGVQLARAFDAHLIGLHVFPAYR
                     LNPPVPLPIGQDILGRIVAQIEEEANRIKSQFDDATSLQTFVSEWRSLRCERTDPAEV
                     VMRHGYAADLLIASQSDPNWEMTSVLDFPDRLAIESGRPVLVIPDGYVFSEPPKSIIV
                     AWKDRREAARAAFDALPLLKVAQTVEVLTVEEEEHREGGPEDIEIATALDRHGVKVSV
                     TTMPASRTPASEEILNRAAQNSQLLVMGAYGHSRFREFAFGGVTRHILKNMTVPVLFS
                     H"
     gene            33765..34154
                     /locus_tag="VF226_09455"
     CDS             33765..34154
                     /locus_tag="VF226_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074253.1"
                     /translation="MKNPEKLLRRVRLELARDPDFPAGSRERGYDFIAPIDEKGQIVH
                     SAWEQLKDRCRVRRFWGTEPDEVGHIVHSPGGEWRFHYDIRGDKRHDEAGYRFSRHSF
                     LPGEYVSILEQDGVMRTFFVASVREID"
BASE COUNT         6287 a        10870 c        10396 g         6623 t
ORIGIN      
        1 gacggcaagc cggcgggcgt catcgccgtt gccgacccca tcaagccgac cacgccggcc
       61 gcgatcgcgg agttgaagcg gctcgggctc cgagtcgtca tgctgaccgg agacaatgcc
      121 accactgccc atgctgttgc gcgcaagctc ggcattgacg atgtcgccgc ggaggtgctg
      181 ccggaggaca agagcaaggt ggtggagcga ctgcgcgccg agggccggat cgtcgccatg
      241 gcgggtgatg gcatcaatga cgcgccggcg ctcgcggcct ccaatgtcgg catcgccatg
      301 ggcaccggaa cggatgtggc catggagagc gccggagtga cgctggtcaa gggcgatctc
      361 accgggatcg tgcgagcccg tcacctctca gcggcaacga tgcgcaacat ccgccagaac
      421 ctcttctttg cgttcgtcta caacgcgctc ggagtgccgg tcgcagcagg cattctctac
      481 cctgcgttcg gcatcctgct gtcgccgatc attgcggcgg ctgccatgtc gttgtcatcg
      541 gtgagcgtga tcgccaactc actgaggctc agccgagtcg acctgaactg agcgctggtg
      601 tggcgccata gccttccccc tcgccgagga attgggcgag ggtccgcacg acacgatcgg
      661 ggctcgccgg cttgcgcacg accggcgggg agccccaggc cgaatggcca gatgtgctgt
      721 gccctgtgtg gatgatgaag ggaatgccgc gctcatagag cagcgcagcc aaggggctgg
      781 cttcctcgga accgaggcgg aaatcgatga tggccgccgc gatggcgttc tgctgcgcga
      841 gggcgagacc cattttgagc gtgctggcag gacctaccac tgtcgctcca gcctctgcga
      901 gcgctagctc caactcccac gcgaggagcg gctcgtcttc gacgagcaga acacgcaggt
      961 ttgagagaca gtcttccatg accttcgcag aacttgaaaa acgcctgtaa gttccgctgc
     1021 ttcgcagtaa gcagaaagtc gcagtgccaa ataatatcaa cagaaggttt ccaggcttac
     1081 gcgtttgcga gacttctcac gggctttcag ccgagaactt tggcctcgca gcctgtcgcg
     1141 aggcttgccg ctgtgtcctc cgaggttggc gtctgcttgt ttcagtcagc cgtcttgaag
     1201 gccacgatcc cgagcatgtg gctggccgtc ttgcggtcct ctgaaaaggg aagcagcagg
     1261 cattcgaact ccagaggctc gccgccgaca ttgatgcgcg cccactcata gcacgggcgg
     1321 cgagaggcga tcgtcgacct gcggcagtcc tctagagaat ccaagatctc gtcccctccc
     1381 cgcttggtga tttctgcctc gccacgccag ccaagtctgc gcctgagcgt ctcgccaata
     1441 tggaggtatt tcacgggctt cagatcggga ttggggccaa gcgcagccag aaacatggcg
     1501 tccgactcgg ggtgcgcagc gatgtcgatc gctgacggcg gcggcaggcc accgctcttt
     1561 tctcgaagct catgccattt gtgatccagg tcattgagct ttccggccac cccatccggc
     1621 agcaggtccg ccgcgtccgc cacgctcatc tccggcgcga tgcaggatgc ggcggcatcg
     1681 agccgccaca caagcccctc acgccgccac tcgagatggg cctcaccttc cagggcccgg
     1741 ccgaccatgg cgtcgataac ggtgtgaccg aacccccgca cccgaggttg gacgacctct
     1801 ggcccgcccg tctcgcgcca cagcatgtgg aaacggtgct cgccgtcgtc gcgctgttcg
     1861 gtgtgccagc gaatctcgat gcacccggtc ggactgctga gcgcaccatg cttggatgcg
     1921 ttggtggcaa gctcgtgcag ggcgagaccg acgttttgag cagcctccgg tttcagcgag
     1981 aggctcggcc cggagaggat gaggccctgc tcgctgcggt cgacgaacgg gcccaaatgg
     2041 gcgcgcacca gctccgcgac cggtacgccc ctccagtctc gtttgacgag cagatcatgc
     2101 gactgagcga ggccgcgcac gcgggcggtg aaccggtcct ggaagtccat cagatcgcgg
     2161 ctggtccggg cggtctgccg cgccatggcc tggatcacgg ccagcaggtt cttcgagcga
     2221 tgcgaaagct cgcgcatgat gaagcgcgtg tgctcctcgc gacgcttctg ctcggatatg
     2281 tcacggtaga tgaccgagac ggcggcaatc cgaccattgt catcgcgcat cggcgaggcg
     2341 ctgatcgaga ccgggatttc cgttccatcc ttccggcgcc tcaacccctc gaactgcacg
     2401 cgcttacccg acttggccag ttcgaacgcc cggggagttg actggccggg gcccggagtg
     2461 aagagcaggt tcgtcccctg gccgatggcc tcatcggccg tgtacccgaa catccgttcc
     2521 gcgccgggat tccagctccg gaggcgcccg tctgccgacc agctgatgat cgcgtctgag
     2581 gacgactcga cgatggcggc caagtgggct tggccgagct gcgcccgccg ccgttccgtc
     2641 acgtcctcgc atatggtgcc gatgccgaca accgatccat cgcgcgattt caaaggatag
     2701 aactgcagca gccagctgcg ctggtcgcct ggtcgggcag gtgtctcccc cgtaacctcg
     2761 acgccggtca ccggctcccc tgtctccaga atgcgtttca ggatcggctc gactttgggg
     2821 cgtaaatccg ggatgaggtc ccaggccaca cggcccagat gctcctcgac cgaatacccg
     2881 ttcattgcgg ccaggacctg gttgatgcgc tggaagcgga actcgcgatc aagcaacgcc
     2941 aagccgaccg tcgccgagtt gtagagggat tgcagctcgt tcagttgctg cgccgtcgtc
     3001 cgaagaagct cggagcggtg ctgctccgcg atgcgagcgg cggtaacgtc ctgatcgatg
     3061 ccgcgtatgc gcacaggctg gccatccgcg gcacactcga tgacgccgcg cgagctgatc
     3121 cagacaagac ggccatcagg ccgacacacg cgaaactcgc aggcaaaggg accacctttt
     3181 ttcgcatgca ggcgggtgat ctcccgaacg cgttcgcggt cgtcggggtg gaccaaattc
     3241 agcaaagcct cgcggctcag cggaaagtcc ttctggtcga ggccgtaaag ctccagcatc
     3301 tccggcgacc aattgctcgc gcggctcgcg aactcgtact cccacacgcc agcgccaata
     3361 ccagcaagcg ccagccgcag ccgttcctcg tttgctcgga tctgttcttc ggctttcctg
     3421 cgctccgtga tatctcgggc gatgctcagc accgactcgg gcttcccact ggcatcgcgg
     3481 agaagctgac tgctcacctc ccagaccagc gtgcgaccgg agcgggcgac cacttccacc
     3541 tcgaagcgcc tctgcaactg gtcctcggaa gccggcaact cacctgtcga gaccggcagg
     3601 ccctcgcagg cggcatgcgc gcttttgtct gacagcaagc tgttgaagct tctgccgatc
     3661 agctcgtttc cgctgtagcc cagggccgag taacctgcgg cattgcaggt taagaagcgc
     3721 ccatcgagat ccgtcgtgaa aatgatgtcg ttggcagcct cgacgagggt tcgatactgc
     3781 tgcctgctgg cctcgagttg ccgcaccgac tcgaagagcg cggaaccgac ccggttcgcc
     3841 tctgccaagc ccgtctccgg gaatcggctc gtgctgctgt caaggggcac accggcgagg
     3901 gtttgaatcg ggatcgcaat ccgccgggcg aagagatagg caagcacgac cgacaagatt
     3961 gtcaaggcag cagcaagtgc cgcaaaagtc acccatcccc ggcgtgcgat tgcggccaga
     4021 tctgccgttg gcacggtcgc ggacacgatc cagccggcca gtgccgagcg gtcgacgacg
     4081 cgaacaactt cggttccatt cgagacgaca cgatggatcg gcatgcccag tctaagctgg
     4141 tccagggtag agcccgacag gcttttgccg acgctacttg catggccttg ggatcgagcg
     4201 atgattcggc ccttgcgatc cgccaatgtc accgtccagt tgagcccgcc gatattccga
     4261 tcgagaattg gcgtgaaaat ctgcgtgggc actgcaaccg tcagcacata ggcggtctcg
     4321 ccccccgata tgaccggaat acggacgccg acgagcggta tattgctggc agaaaagaac
     4381 aggtcagaaa tttgcggctt tccggtttcc attacatctt cgatgcggga caaatcggtg
     4441 gtcgggagcg tgctccctga cgggaaagtc gtgttgacga gctgcttgcc agtgcgatca
     4501 gtcaagatca cgtaccagcc atggagcccg aatagctggc gagcctgcag gtcgaaagcc
     4561 tcgaattttt gctcctggag caatggcgag gaggccaaag attcgccaac acgggtgaag
     4621 cccagaattt cacggtcgat cccggcagct atgtagctgg catctcccag aagccgcatt
     4681 ttcgtgctgg cgctttcggc caagaaaagg ttccagagcg ccagtccacc aaggatgatt
     4741 ccgggaacca atatcgagag gcaaagcacg agcagatgcg tcgacaggga ccaggcccgc
     4801 ccatctactg gacgcgtttt aaatcgggcc acgtgacctt ctcgaccctt ctttacctca
     4861 taaggcctcg ccgtaagatc cgatatcagc cgcctcgaat tcgaacctgc attttgcacg
     4921 accgacctac gctgaacttc cgtagccgag caccggctgc atccatctaa aatcggaact
     4981 ggcacagcgc gcccgcgttc ccttgcttga ccgccaatgg agctttcgcc gccgtttggt
     5041 tagctgcggc gccggtgcat tgagggagat tgtgatggtt tacggctcgg aaagcgtgaa
     5101 cgaggcagct ggaaaggaaa ccggcgacat tggtgcgaag atcgaggagc tgcgagcgga
     5161 aatgatgcgg ctttccgagc aggtccaggt ttatcttcag aaccgcgcag gagacctgaa
     5221 agaagacgca accgaaacgt cggcagaggt tcagaaactc attcgcgaga atccgtttcc
     5281 cgcagtcggc gtcgcgctcg gcatcggatt cctgctgggt ctcatgatgc gaggcggagc
     5341 cagcgctgca ccgcgcctgt cacggcgcga tttcgatcgg ctggcctcca aggtccgcgg
     5401 agcgatcgag gccggaagtg cacgtgctca ggccgcggca tccgaagtcg gggatacggc
     5461 gcttctcgag cggctcgccg gagccctttc cagcgtcatc ggatcctcgc gatcggccgc
     5521 ggaatcggtc ggctccgcag gcgggcgggc cgcaaaaagc gttgcagccg tcggggaacg
     5581 gacggcccga aacatcgccg agcgcctgtc ccaggccgca cactaaattc cggctgggcc
     5641 gccggctcac tgcaggtggc cgaattccag cgcgatttat tgatcaacat tcggtgaggt
     5701 tcatctggcg aagcggggat tgctgagcgc attaaccgct tcgacgtgcg gttcagaagt
     5761 gcgcacacgg tccaatccgt attgccaatt gatcgcccga gacagacctt cgagccccgg
     5821 aaacatcgac gcgtggctga tgccgaaacg ggcaagatcc ttgagcagca ccggccggaa
     5881 ggatgaatcg atatccactc gatagagctc gccgtggctt tcggaaaccg gctccgaggg
     5941 cggtccatgc accgtaaaaa cagcctcctg ccgcgatatt cgcgccgcca gggtccgggg
     6001 ccggtaaagc gccacgcccg tgaagctgaa gggatcggca tccgcttctg tcactatcct
     6061 tcccgggatg aacacccaca aagctgcgga cccgggccgg ctgctcgcag ctgcgaaaaa
     6121 cgccgcgaca agcgggttga tggtccagtc cagaagtcgt gtcgccagcc cataatgccg
     6181 cgcgatcgtc aggcaatccc agtcatctgc cggaagatcg agaaactgga cggcttggcg
     6241 ctgccactcg cgaaagagct gaatttccgt ctcccggtcg caatccgtgc gtccaaggtg
     6301 gggcacgagg gcctgcgcgg catccacgtc gccgcgaaac accgcgggtt gactgagcgg
     6361 cgtcaccgca tcgtagagtt cggcaaaaga tgaaacgagg acagctttca atcgtcaccc
     6421 actgtgcgga gcgcttcggc gcgctcgcag accaacgcgg gtcaattgac acgggatccc
     6481 cttcggatgg cgctggagtg ctccagctct ggagccgcac cctgcctcta gcgcggcctc
     6541 aggcctttcc ggcgatcaac gcttcaacga ccgccaccag ctgccccgga ttgaatggct
     6601 tgtcgatgag cggcgctgtc cggaattccc gcggtatgcc ttcgaggccg tagccggtca
     6661 aaaacgcaaa tgggattccg cgccgggtga gctcggctgc caattcatca accggctcgc
     6721 ccgagagatt ggcgtccagg attgccactt cgaattggtc gccggcgata agctccctgg
     6781 ccttgtccag attcgccgct ggtccgacga tgccgcagcc ggcatcggac agcgccatcg
     6841 cgacgtccat cgataccaat ggctcgtctt cgacgatgag aacccggggg ttctttcctg
     6901 gtgcggatcg ggcgagcgag tcccccagcg aagcagtcgg tcgtgctgaa tcggaggtca
     6961 cgcgccctgc ggatatggcc ggacgccggc tgtccggcaa tggaaggctg atctcacagg
     7021 taaccccctc caccgggtat gcgacagaaa cccggccgcc ttgcgcctcg aggctctgct
     7081 ctatgagcat gctgccgaat ccgcgctgac ggggcggttt caccggcggc ccgcctcgct
     7141 cctgccaaga taacagcagc ttctcatcgc aaccgcctct caccgcccac cgcagcatca
     7201 agcgtcccgt tggaacagag agcgccccgt acttgcgtgc gtttgtgccg agctcgtgca
     7261 gcaccagtgc gagatgcagc gcaacctggg gctccaggac gacctgcggg cccgaatagg
     7321 cgatctgctc gtcttcggcg cctccgagca ggagctggtc gcgaaggaga tcctcgacgt
     7381 cgaccccctg ccagtttccc cgagtgagcg cggagtgagc gcgcgcgagt gattgcaggc
     7441 gtcccgaaaa gcccgacatg aattctgctg ggctgttggc cgagcggacc gtctgggtcg
     7501 caatggcctg gacggttgcg agcgtgttct tcacacgatg attgagctcg ccaatgagca
     7561 gcttctgcag ctcttcgtga cgtttgcgct ccgtgatgtc gcggctgacc ttcgaggcac
     7621 cgatgacccg tcccagtctg tcccgaatgg gcgagacagt gagcgagatg tcgacccggc
     7681 gcccatcttt ggcgacccgc accgtttcga aatgatccac acgctcgcca cgcctcagct
     7741 tttcgagcat cgcgagctct tggtcgtgca gctcaggcgg aacgattagc gtgatcggct
     7801 tgccgaccac ctcctgctct tcgtagccga aaatgcgggt ggcgcctgcg ttccaggacg
     7861 tgatccggcc atcgagcgac ttgctgatga tggcatcatc ggatgaggcg acgatggcgg
     7921 caaggcgtgc gagttgcgca tctgtctcat ggcggtcggt aatatcgacc atcacgttga
     7981 ccgcgccaat cagggagcca tctccgcctg atatgggggt tggatgtggc aggacgctca
     8041 cacgcgttcc atccggccgc tcgatgatca cttccatgcc ggaaatgggg cgctgctcct
     8101 tcaatgcgac ggccatcagg cagtcctctc gtgggatcgg ctcgccctcg ggcgtgtaaa
     8161 tccgccacgc ggagcagaac cggtccacgt caggctccgg cttccgccca ataagccttt
     8221 cgtaggcctc gttgaagaag atgatcctgc cttctgcatc cgtcatgtag actgcaacag
     8281 ggactgaagc cagcgcggct cgcgcacgtg tctccgtaat gcgctcgcgc gcttcagcca
     8341 gtttcctgcc ggtgtcatcc aggacgaagc agcgggtctt gaccaatttg ccgtcaagga
     8401 cctcggcatt ggacgtaagc agcacgtgcc ggatcgaacc atccttggcc cgcatgcgag
     8461 cgggataacg gtccaggacc tcgtcacgcg acagccgagc caagatctcc tcgaccacgt
     8521 gctcgtcggc atggaaatcg gcgacgggcc ggccgacgta ttcctcggcg ctgtagccca
     8581 ggagctccag ctcggccttg ttcgcacgga ggatcgtgcc atccccggcg acgacgtgaa
     8641 gaccaattgg cgcatgctcg aagaagtctt ccagaacctc gttcccgctg tttggcggtt
     8701 catccttgcg gctgaggttc gtcacatcct gaaagcaact gacggcaccg acgattttgt
     8761 cgtcatcgcg cagcggctcg gcatttacga tcaggctgac gcgcgtgcca tcggggcggt
     8821 ccaccatcac cgttgcattg cgaaccggct taccggtctg cagaaccctg gcgatcggga
     8881 tagcctcgag cggaagctgt ctgccgtccg ggtcgaagat gcgctccgcg ccggtgaagc
     8941 gctgcgcgcc aacctgcggg cttcggcccc aaagctcggc agcatgttta ttgaactgga
     9001 ctagaacccc ctcggcatcg cagcagcaga taccgaccgg caggttatcc aacagatcca
     9061 cgggaccgaa ccgatcgcgc agatatcgcg tggtccggct cacatggagg gataggacct
     9121 cggtcatagg ggaactcact tttacagact gaatgaacgc acccgagagc ttgtcggttc
     9181 ccggcaccca agccagcagc caactctcaa aatgactact gaatgtttga agttcgccga
     9241 atgtttgatg atggggcttc cagcctcaag ggcaatccgg aagtgtcgcc gtgacgcaac
     9301 agggaggacg gtgcgccact gggggtttcg gggagtatgt cgcggattag gcggccatat
     9361 tccggcggga gtggctgggg cgggaggatt cgaacctccg catggcggta ccaaaaaccg
     9421 ctgccttacc gcttggctac gccccactgg ctggcatcct gtagcgaggt ttgggcaggg
     9481 ttgcaaggtg ccgctggagc ttgatcgcta gactcggcga gccccgcaga tggctcattc
     9541 catgcggggc tcggctaccg tcttgggagg cggccgcgac tgttatgcgc cttgcacgcc
     9601 gtcagagccg tccccgtcgg gcgggttctc cacctttacg acagtcaagc gacgctcctt
     9661 gccgtcgcgc gccgcccacg cgatcgattg gccttcggac agaccgataa gggcggtgcc
     9721 gatgggtgtc aggatcgaga tcttgccggc accgatgtca gcttggccag gaaagacgag
     9781 ttgcacacgc ctctcgcgcc cgtcgtcagc cgtgtaggtg accacggagc ccattcgcac
     9841 caccgatgaa ggaacatccg gcaccacgcg tgctcgctcc atctccgcga tcagctcctc
     9901 ggccacatcc ggagaacggt tcagcgcatc catggcgagc acggtgagcc gctgttggtc
     9961 ggcctggctc acaacgatct cgggcatttc aggataatgt ctgtctttca tgggctcacc
    10021 catttgcgct tcgaaaaatg cgacgttctt ctctatcagc gttgttgttg tcagtgtcgg
    10081 gaggcggatg tgcgcaatcg caaccgcatg gccggtgcca tgctgtgcga tgagccgtgc
    10141 gtgtaaggag cgccccaggc tcgggagcgc acggttgccg agctggggca gctatcctgc
    10201 gatcggatgc acccggcgcg ccagggacgc gcgatgtacg agattgctca acataagctc
    10261 taatctggtc cctgcgatac gaatgtcaag ccgtatcgat caggttaaac ataaactcca
    10321 ttcggcctcg aggaatgagc atgcggcaat tcgggcgatt cttcggccgt tcctgtccat
    10381 tcgccggcac gtccgttctg cggattgcgc gattcaccgc ccggcctgcc atccccttga
    10441 gagccggcca attgcccgtg aggcgacaaa ttcggagtgc tttgtgagcc ggtcggctgt
    10501 gcatggataa tattcgccag ggcggcccgg tccggctcgg cgatgtggat cgtgaggcca
    10561 tcgacatcat atccggccgc ctgcaagagg cgcgacagcg tcccctgatc cctgcgaatc
    10621 agctctgcgg tggctgcccg ctgcgcctcg acacgcagct ccagcgcgcc tgattccaat
    10681 ctcatgcgaa tggtgaccgt cccgagctca gggggctcga ggcggatctg gagcacacga
    10741 atgatcgtat cgcctggctt cggcaactgg ggtactgcgc gcgtgctggc agtttgccgc
    10801 tcaatcgctc catcggatga tgcgatgagc acgtgcgcga tttgctccgc ggcaggaagg
    10861 cgctggacca gtggaacatg cacctcctgc cgggtgatcc gaacgggaat gctgcggtca
    10921 gaatcaggac gatagctccc gatggatatg gctccagtct gggggcgttc acccgaactt
    10981 ccgggcttcg tcacgagttg ggattggcca gggtgtgctg aacgtgcatc ggaaatatcg
    11041 gccggtcgag gctgatgcgc cactgaaagc tcgttcaatg gagggatttg cgtgtcgtgg
    11101 cgtctccgaa ttggtcgcgc cagctcgtga ttgctgctgc ccgaatgcgt acgcggcgcc
    11161 gccatcgcca acgcgtcggc atgggatgca tcgcgttcgg ccaaggcgat cgagccgatt
    11221 tccactgctg acatttccgg gttttcttcg ggctttccgg cgagccgaag aatggcatcg
    11281 ccgagtggcc gcgcgagcaa cgggacgggt cccgtagaga ttggtttttt tccgaagtcg
    11341 cgacgtggcg actgactggt ggaagtgacg cattcacttt catggttgct cgccactgcg
    11401 gatgcgccag cctctggaag ttctatcaat gcgatcatat gctcgcagaa ttcagccccc
    11461 gactgctttc ggccgtctgt ccgcccgctt ctcttcaagt gaaccgacgc caagccttgc
    11521 gcgtcggcac tgccgctctt ggggacgttt ccggtgctgc tcattgcggg ccctcgttca
    11581 acagagcatc cacatccgcg atcatggcgg cagcacgctc tatcgtgccc cggccaattt
    11641 gagacagctc gcgcgcgggc gattccgcct cgccaatggg ggtcgaggct tgtccgtcat
    11701 cggagggcaa gcgacgaatt tgcctcgcca cggagagagc cgcttccctc aacccggcat
    11761 cctcgtttgt gaattgacga gcgttcatat tcatcaacat ggagacggcg cgatcgtagt
    11821 cgtctgtcag gaccagagca gcggcctcgt agagactggc tcgcactgca ttaggctgcc
    11881 agagtgctgc gcgtgggact gcgaattgga cgagaaagcg attcccacgc gcgatcccct
    11941 gccgtgcgag cgacagataa atccatccct ggacttcggc atcagccttt ttcaagaagc
    12001 tttcgagttc cgctagatgt ttggaattct cggaagtggg ttgctcggcg agcttagctg
    12061 cgaactgatc ccagaacgca atcgcgtaat ccgatctcgc aaagcggcgt atatagcgcc
    12121 gcaacaaaaa atttgcgcgc tctggctcct tggcttccag cagcaaaaag atctggcgcc
    12181 gcagagctga ttcctccacc agcgtacctg gagacagcag gcgagcctga tcgagaaggc
    12241 ccaggactct tgcaggatga tcgctctccg ccgccagtga agactgaacg agcgcaacgg
    12301 cgccggcgag gctgggatca agactccgcg catcgatccc ggccagatgc gcacgcgcct
    12361 ctaagctccg cccttcggcg taggcgagga cgcccgctgc caattcggtc tcagcatccg
    12421 gaaaccgccc ttgactgagc agcgtccgca agatcgctgg cgcaccgccg ctcagtacga
    12481 aaagaacggc cgctcgtctg ttgcgtgcat cgccccaact tttgggcgcc gcggccgcca
    12541 acttggcgga caaagccgct gtcagatcat gctgggcggg ataggctttg cgccttcccg
    12601 ctgcgacctg gtcctgcaag cgagtaagct cgcggacaag ccgatatggc tcctccgcaa
    12661 gctcctctcc gcgcgagagc gttgtcaccg ccaacagcgc tgccaatccc agtgctgctg
    12721 accgaatcca gatcaagacg tccccgccgt tcgcaaaagg atttcgattc gcctgttctg
    12781 agccgcttcg gggtcgctgg gtaccttcag gttacggtct gcatatcctt cgatccgctc
    12841 gaaacggctt tcgtcgaggc caccacggac gagcatgaaa taggccatat gcgcccgggc
    12901 cgatgaaagc cgccagttgt cataggtttt cgttctgaag ggccgcccat ccgtgtgacc
    12961 gcgaacgata agaggtccag gataatcttt cagaactttc gcaagctgct ccatgatgac
    13021 gacgagctcc ggactgggct cggctgatgc aatcgcgaac atgccgaagt cgaactcgtc
    13081 ggtcaggctg atcagaacgc catccgaggt gcttaccacc tcgatattgg gctttctccc
    13141 cgggatcgcg ttgttgacac tcgagcggat cgcttctgcg atgtcctttt cgaccggcgg
    13201 aggcggattt ggctctttag ggccggccga aggagctgca ccaggctcct tcggtggttc
    13261 agcagatgtc ctctctatcg cttcgcccga ccccagccga ctcttcggtg cgatcgaagg
    13321 atcggacggc ggttccagcg aatgctccag tggctcggcg aatgactggt ccgtgccact
    13381 ttcttcgaga ggttgcggag ccggcttcaa ctcgccggca ttggctcgtg ctacgaggaa
    13441 gcggaaatcg ggatcgaagg ggtcgcggaa agccacatcc gcagcttcct ttttattcct
    13501 ctttacgtcg ggttcgtccc gcttcgcccg ttcggccaat tgggccagca ccttataggg
    13561 atcactgaac agcgtctcct cccggaaagt cgggcgaaaa ctgccttccg gagccacctc
    13621 acccgtggga ttgcccttga tgcgagtcgc ttccactgcc gggtcttggg cctgatgggc
    13681 atcggcctct atgtcgttca gccccttcgg ctttcgcaca tgatcggtta gtttcaccgg
    13741 gttgaagtag ctggcgacag cggagatcgt ctgcttgtcc gtcgcattga gcagccacat
    13801 caccagaaag aaagccatca tggccgtcat gaagtcggca taggcaattt tccagatgcc
    13861 tccatgcgga tgctcatgtt tgcctcctga tcgccggcga atgataagca gttcttgaga
    13921 aagttcattc gggttacgcg gtacaaaatc cgaattcatg tttgtctcgc ctcacgcatc
    13981 cactgtttga gaattgccgc cgcctgatcc tcatcgaagt ccacaagctg ctcgagccgt
    14041 ttctgcggtg agcgactgag cctgctcttg agatcggcga tgaggttcgg ttctgccgcc
    14101 ggcaacgtcg gctcacctgt tgcaggctca cccccggctc ccgcttcgag gcgtccggcg
    14161 gcggaaggcg ctccggcagc agatccagcc tcgagaatca tgcgcatggc cgggcgaagg
    14221 ccaaaccaga cgagcagcgc cgtcgcgccg agcgcaacga atgcattgac gaccgtgccg
    14281 gcctggctca aaagcagctc gccgattccc atgcctactg gctctagcgc ctctccatcc
    14341 ttgaagaagt ccaccgcagc gacggtaatg cggtcaccgc ggtcggggac gaggccgact
    14401 gccgagctga cgagcttctc tatttcactc aggtgcgctt cgatcgctgc ggtatctgcc
    14461 tcctcgccaa tggcggagag aaggcgcttc cgattgacga cgaccgcgac ggtcaatgcc
    14521 tcgatcctgt atccgtcgct gacggtcgaa accgttttgg agttcagttc gtagttcgtc
    14581 agctcctcgc ggcgctcgtt gctgcgcgtc gacacgtcac ccgaacgtgt cgtcagatct
    14641 tcctccggca gattctgctc cacgctgacc gggcttcggc tcgactggtt ttgcgagctg
    14701 cccgactcac gaacggtacg gacagatcgc tcgacacgcg aatccggatc gaaagccgtt
    14761 tcgttgatct gtcgcttgtc cgtgttgagg cgcgccgcca cgctgacctc gaaattctcg
    14821 attccgaggt aaggcgaaag cgtttttcgg gcgcgctcct gcagctcctt gctgatcgtc
    14881 ttttccagct cgacaatccg cgccggtagt gcagtgctcg cctcgcctgc ggcggccaga
    14941 atcgtcccat cggtgctcag aacagtgacc tgatcgacgg tcagccccgg aaccgcggca
    15001 gccacgagat gccggatagc cggagctgac gcgaagtccc cggccgtttc ggtccggatg
    15061 accacggacg ccgacggctc ctgacgattg cgccggaacg agcctgcatc tggcaatacg
    15121 atgtgaacac gcgcggcttt cacacctttg acggcctgaa ttgtccgcgc gatttccccc
    15181 tcgagggctc tgacccgcgt aatctcctgc atgaacgagg tgaggccaat agagcccaga
    15241 ttatcgaata gctcgtaccc ggcattggcg ctgctcggta atcctttctc ggcaagcagc
    15301 atcctcgcat gtgcggtctg ctcgcgccgt actagaatct tgctgccttc ggcattcacg
    15361 tcgaaaaaga tgccggcctc gttcagcacc gcgccgatac ggctcacatc ctgtgcgctg
    15421 agaccgctgt agagcgtctc aaggtccggc ctgctcagga aatatccgat caatccaatc
    15481 gcgccaaatg ccgtaatccc aattacggcc agagccgcca agcgacgctt gcccagcgcc
    15541 aggatgttct ggaccagctg ctcgatctgt tctcgcccga tcatgagaat catccgtttt
    15601 tcgtcgattc atgcagcgcg atgcttgggc tcgaaccttg cgtcaagata aagggaggag
    15661 atgaactgcg cccccttcgg ggcgcagccc aagctcgcct ctggcacgct gggggagtgg
    15721 ccagagtcga gacaagccgc agtgccggcc aaaactcagg caggcactgc caggcgccga
    15781 cttatcgccg cttgcttaaa acgcaacggc tgatcccgcg ttagcggaac agcgccagaa
    15841 tgttttgact gttggcgttg gcaatgctga gagcctggat accgagctgc tgctgaacct
    15901 gcagcgcctg cagcctggtc gattcttcgt tcatgtcagc atccacgagc tggccgatgc
    15961 cgcggtcgat ggcgtccatg agcgaatcga cgaaatcctt ctgcaaatcg atccggctct
    16021 tgacggcacc gaggtcggac gccgcggtcg ttacattcgc agccatcgca tcaaggcccg
    16081 cgatgtaggc gtcgagctgc gcgtccgtcg cattggagat atcgatatcg cttacgccga
    16141 agaaattccc gttatcgctc gtcgcggaat tcaggacagc gagcgtcgca tccgtcgcgt
    16201 cgcttacgtg ggtgattgcc acacctcccg agccgtcagc cgctaccgtg acgtcgctgg
    16261 ttgtgtcaaa tccagcattc tgcagcgcca gtgcgacgac gctggccatg tccgtcgagt
    16321 ccgccaccac cccgtcgctg ctgccgagcg ctgcgtcgac cgtcctcttg tcgatggtga
    16381 tcgttgtcgc ctcaccgttg aagaccaaat cgaactttat tgcgtcgttc tcgtcgaggg
    16441 tcactccgtc cgtatcgacg tccgtgaaca gggtgacgct cgcaggagtg ccgctaccgt
    16501 cgccagcaac gggcgtcacc gtgtcctgaa gaccgccgtc ggcagtcagt gcctggccag
    16561 catccaggag gccggcaccg gtcgcgtttg cgtcgaacag agcggttttg gtcaggtcga
    16621 catcgatcgt gccgatctta atagttccgc tccccgtgcg ggagaatgat gaaacgacct
    16681 tctggatagc gttccagcct gcggccgaag agtcgaccga cagccaattc gagcccgaaa
    16741 aaacggcggc ggaagcaatg ccaacgagct ggtcctgcag ctggtcgatc tcgctctgaa
    16801 ttttcgtctt atcgacactc ggctgtttcg ctgcgaccag cttgaccttg atctcgtcga
    16861 tgatgtcctt ggcgctttcc atggccgtat aggcgatgtc gacctgagcg gcgcccagcc
    16921 ccaatgcgtc ctgcaccgtc gacagcgcct tgttgtcgga acgcatggtc gtcgcaatcg
    16981 accagtaggc cgcattgtcg gaagcggtcg caacgcgata acccgtcgag atccggttct
    17041 gcgtcaccgc catgttcttg ttggtctgcg tcagcgtctg cagcgcagtc attgccgaag
    17101 tattggtgag aaggctggtc atctggagat gtccctctac tgcggttcat agtgtttcgg
    17161 ggacataccg gatcggccgg tatggcagcg cggcctcatg cctttgggct tttttcatcg
    17221 atgcccaagc tgccatcgat catcagaatg accgacagat cttgcttctg gcttaacgaa
    17281 atagctggca atcaagcaaa cgagcgtatc agcccgccaa ccaggaggtt ccagccgtcg
    17341 atcaggatga agaacaggat cttgaacggc agggatatga cggtcggcgg cagcatcatc
    17401 atacccatgg acatcgtcag ggtcgcaacg atcatgtcga tgacgaggaa cggcagcacg
    17461 atcaaaaaac caatctcgaa ggcgcgcctc agctccgaga tcatgaacgc cggtatgagc
    17521 acgcggagct cgattggctc atcgggcgag cgggtttcca cgccggctag ctcctcgaac
    17581 aggctcaagt ccttctcgcg cacatttgct ttcataaagg tacggaacgg ctccgttatc
    17641 cgcctgtacg cctcctcctc gccgatcttg ttctccacca gcggcttcag cccgttatgc
    17701 caggcctggt cgaaggtcgg cgccatcacg tagaaggtca tgaacagagc gaggctgatg
    17761 agaatcagat tggccggcgt ggtctgcagg ccgagccctg tcctcagaaa cgaaagcgcg
    17821 atcacgaatc gcgtgaagca cgtcaccatg atgagcagtc ccggcgcgac agacagcacc
    17881 gtgatgatga tgacgagaag gaaaatctgc cccgtcgcgg tcccctctcc tggcggcacg
    17941 acggcatcca ggccaagggt ttgcgccccg gctggggcgg taccgaggat cagcaggagc
    18001 gcggtgagcc cgacaaatct actcattcga caataaaggt atgaacgatg aaatcccgga
    18061 cccgcccatc gctgcggatg cgcgctcttt cggcgaggtc ctcccgcagt tgcagaaagc
    18121 cacttggtcc ctcgatgtcc gaaacagaga cagtctgcag gaatgccgcc atgtcctcgg
    18181 ccattctcgc cgccatgatc tcctcctctt cagcgggact gcctgccagt acgatcgagg
    18241 cctcgatgcg tacccaggca tctcgctctc ccgcgagttt cgtgacgacg gggggaagtg
    18301 cacggatcct gcccgattcg gaataggccg gagccggagt gacaggccgg ctggtattca
    18361 cttcgtcctg ttcgcgcttc ggctgctcgg caatcgtctc aatctgcaat ccaaaaatgc
    18421 ccccggcgag caccgcgagc agggtggtcc cgatccaagc agcaatgacg gccgccgggc
    18481 cggtccgtct ttctcgcttt ctgcctcgct gcggaaaaac tcttgctatc tcgaccatcg
    18541 cttgccgccc ccgcaatctc agaacggaat taagagatcc aggatttgct ggcccactcc
    18601 cggctgctga acctccgtga tccgaccgcg gccgccatac gaaatgcgtg cctccgcaat
    18661 cttgtcgtag gcgatggtat tgtcggtctc gatgtcccga gggcgaatga caccagcaac
    18721 gttcagaacg cgcacctcat aattgacccg cacctcctgg cttccgctga tgacgagatt
    18781 gccgttcggc aaaacgtccg acacgacggc tgccacgcgg agctgaatgc tttcagaccg
    18841 cctgatcgcc ccttcgccct tgctcgtcga tacggaatcg agctccgcat cgagctcccc
    18901 ctcccattcg gagctgctgc cattgctgcc cgcgccgtag cttccgagca gccccagctt
    18961 cttgttcgaa tcgcgtttcc ggttggatct gttgtcgagc gaggccttgt ccttgatggc
    19021 gatcttcacg gtaaccacgt cgccaattgt cctcgctcga ggatcgccga aaagatcagc
    19081 gctgctgtcg tcccaaagcg aattatcgcg ccgaaaccgc gggaaaggtt ctggttcgcg
    19141 caaaaccgga acgatgtcgg gcctaaggcc cgaaccaacc ggggtcagat gcggctcgcg
    19201 accgatgtct gctggatcga ccgcgcaggc agacaggatc ataccgatga aagccactgc
    19261 cgccagggac ctcatgactt gtccccagtc cgttccgtat ccttgctggt attcactttc
    19321 gctgcaccgg cgatggtcat ggtgaggcgc gcagcgacgc ccggagccat ttcattgaga
    19381 attgcgctcg cgagccgcgg cttgatcttt gtgaggacgg cggctgccgt ctcctcgtcc
    19441 atggctgaaa gctgagaggc cgccgcatcc ggccgcattc gttcgtaaat gctcacgaga
    19501 gtttcctgcg ccttcttcac aaaagcatcg cggcgcgcca gccactcgcg gagctccgcc
    19561 gtccgtttct cgaggacggt aatgcggcgc tgaagctcag catccatttc gctcaacgta
    19621 cgcttctgcc atgcaaaccg cgcgtcagcc gcagcgtcgg cgatatttgc gcaatattcc
    19681 tccgcgagcg cggagcgggc cgggtgacct tccggtcttt tctccgcggc ttcctgagat
    19741 ggagcctcgc tctgatggac gagcttccga gcgggttgct cctgggtgct ttcctgttgc
    19801 tccggccggc tctttgttgg cgatgatggc cgagacacct cggcggattt ggatgagttg
    19861 atcaccaccg gcttcggcgg gttgatgatg accggaaacc aaccctgctc cagctcagcc
    19921 tcgttatcga ccggagcggc ctcggccgtc ggaattttcg gcctccacgc ctcttgtgcc
    19981 agggccggcg ccatgccgag gcaaagcaat gccagcgcgg ccaagccgcc atgggtcgcg
    20041 ctccttttcc ttggcattta ctgcaccacc agctcggcct gcagcgcgcc cgccgttttg
    20101 atcgcctgca gaatggcgat gataccagcc ggtttcaggc cgatcttgtt gaggccgttg
    20161 acaagcgact gcaaattcgt tcccccgacg atggccatgt ggccaccctc ctgttcggcc
    20221 gaaatcagcg tttccggcgt cgtcaccgtc ttgccctccg aaaagggtag cggctgcgaa
    20281 accctctcgc tctcggtcac gcgaacagtc agattgccat gcgcgatcgc aaccgtggaa
    20341 atctgcacgt ccctcccaat gacaatggtt ccggtgcgct catcgatgac gactcgcgcc
    20401 ggcgcgtccg tttcggtcag gagatcgcct atttcggcga tgaatcgtgc agcgctgaca
    20461 ttcctcggct tcagcagctt caccgagcgc aaatcgtccg cccgtgcggt cacgacgccg
    20521 tatttttggc cggtgtaggc attgatggtg tcggcgatgc gcaccgccgt accgaagtcc
    20581 ggactgtgga gctcgagcgc gagaattccc attccggcca atgttcccgg tgcctcgcgc
    20641 tcgacgatgg cgccattcgg aatgcggccc gtcgtgggaa cgccctgggt gagcttttcc
    20701 gcgttcccgg tcgatgcaaa cccggtgaca gcaatggggc cctgggcaac ggcatagatc
    20761 gcaccatccg gccctttcaa aggcgtaagc acaagggagc cgcccgccag cgaggttgcg
    20821 tctccgagcg aagaaacggt cacgtcgatg cgcgcgcctt tcccggcaaa tgccggcaac
    20881 tctgcggtga cgatgacggc agctacgttg cgggtccgcg ccttggcgct gcggatatta
    20941 atgcccatgc ggtcgagcat cgcctgcagc gactgctcgg tgaacggtga attccgcagg
    21001 ctgtcgcccg taccgctgag gccgaccacg aggccatagc cgatgatctg attggatcgc
    21061 acgccttgga ccgacacgat gtccttgatg cggctcgctg cggcggcgtc tgcaagcccg
    21121 atcaggatga gcagcacgac aagtccggcg cgcctcacgg ccccaccctc acagtgccgt
    21181 ccggttgaac ggcgccgctc acgatcacac cgctgtccac gttgcgcacg cgcaccacat
    21241 ccccgttccc cccggactgg agcgcgactg catatcccgt gatgacgagc gggccatcct
    21301 gaaaaacgat ctgaacggta tagccctgga ccaccaaatg cggttcacgg acggcattga
    21361 tgggaatggg cttgcccggc agcagcgtcc gccggacaac ctttccgata agtgcatccc
    21421 gttttcggtg gaccggactg tcctttttca atctcacatt gaaagtgcgc tcgacgagat
    21481 gatcggtccg tatgaaatcg cccggataga gcgtgatctt cggaaccggc aaggatatgc
    21541 ccgcggcgtc aaccgggctg gcccgcagga gaacgcccgt gaaaatgagc acattgaacg
    21601 cgctcctgag aagcgccatc caaaactctg gccggccgcg gtccacgctc atatcccact
    21661 tcagcggatg cctttcgtga tggtgccgag catgtcgtcg gcagcctgga tcaccttcga
    21721 attcatttca taagcccgct gcgccgaaat cagctccgtg atttccttca ccgggtcgac
    21781 attggagttt tccagatagc cttgctgcac cttagcgaac ccgatgtcgc ctggtacgcc
    21841 gatgacgggc gcgccggatg cctcagtctc gcgatagaga ttgccgccca gcggctcgag
    21901 ccccgcttcg ttggcgaaat tggcgagcgt gaattggccc agcaattgca gttccggttc
    21961 gttttcgatg cgaacgaaga cttcaccgct ctcgttgacc acgatgttgg tggcgtcatc
    22021 cgggatagtg atggctggct gaacgacgta gccgtcgatc gtcacgatct gcccgtcggc
    22081 attcttgttg aaggctcccg cgcgggtata gagttgctcc ccatccgcac tggccacctg
    22141 aaaccacccg cgcccgttga gtgcgagatc cagcttgttt gtcgtattgg tcaacgagcc
    22201 ctggatgtgg agcttgcgga ttgctgcagc ccgcacccca agccccagct gcgctccttc
    22261 gggcacggcg ttttcgccgc cacggatcgg tgcacccgcc gcacgctcgg cctgatagag
    22321 gagatccgtg aattccgccc gcgcccgctt gaacccggtc gtgtttatgt tggcgatgtt
    22381 attcgcgatc acctcgagat tgagctgctg ggcagtcatg ccagtggcgg caatcgctag
    22441 tgctctcata tttgctcggc cttttctcga agtgtggtca ggtcggagaa tttcgtttcg
    22501 cacgcctaaa tcgccatatg gctgagctcc agataagccg cggtcacctt gtcacgaatg
    22561 gcaatggctg cctgcagcgt ctgttccgcc gcaagcacgg attcgacgac ctcctgcacc
    22621 gaagcctcgc ccattatgcc tctgaccgcg ttggattcgg ctgcgcgcag gacgtctgcg
    22681 ctatcggtaa tgagcttgcc cagcaccgtg ccgaaatccg cagcctggga atgcgctggc
    22741 gacgccaaat gaggcgcacg ggtggccgtt gaaactgcca atggactccc ggcgccttgg
    22801 gagacgaatg gtgcgatctg gtcaatcatc acgagttcct cagcagatcg atcgtcatcg
    22861 agagcatttc gcgcgcctgc ttgatcactt ggagatttgc ctcataagag cggttcgcct
    22921 ctcgcatatc cgccatttcg acgagcatgt tcacgttcgg cagcttgacg taaccgctgg
    22981 catctgctgc cgggtgcccc gggtcatgct cgatgcggta tggcgaccgg tcgaaatcta
    23041 tgctctcgac ccggacgaga tttgccccgg tgatccgatc gacctccgtt tcaaagctga
    23101 tggtcttgcg cgtataggga tcagcaccgg gcgtgttgcc ggtcgactgc gcattggcga
    23161 ggttctccga aacgatacgc acgcgcttcg actgcgcctc cagtccagaa gcagcgattt
    23221 tcagggacac ctgcagcgga tcgatcattg tcattccttg actagccttt tgcgctggca
    23281 agcatcatgc ggtggaaggc cttgatgata ccggcgttca gcgcgaacga gcggttcacc
    23341 tcgccggcct tgatcagctc ttgctccagg acgaccgtgt tgccggaatg ggtgacctcc
    23401 caggcgtcct catcgctcac atcagccacc ggaatgccgg cgctcggcgg gctcatgtgc
    23461 cccggctgcg tcactgccaa ttcgaggggc gcccggcgca gaactgcctc gaacggctcc
    23521 gtatcgacgg ccttgaagcc gggcgtattg gcattggcga tgttgcccgc cacggtggtc
    23581 tgccggacgg atagccactt gttgtgctgt gagatgaggt cgaagagata gacaggcccc
    23641 atgcgcggct ccctcgtcgc gcttcaaatc atgaattttc agttaaggga gctgtcttgc
    23701 ccgagccttg tctttagatg tttgcggcga acgctgatat aaactcaatg ggtcttcgcc
    23761 ttaccccata acgcttccag acgggcgtcg cggccacaac cgctgcgata gatgaaatat
    23821 cgaagggggt tcttccggta ataatcctgg tgataatcct cggctggggt gaacgtggac
    23881 gctgccgtga tctcaaccac gatcggctga ctgaagcgcc cgcttttctc gagcgcggcc
    23941 ttggactcct cggccagccg cttctgctcc tcgttgtggt agaaaatcgc tggccgatat
    24001 tgatcgccgc ggtcgcagaa ctgtccgcct ttatccaacg gatcgacatt gtgccaatag
    24061 acgtcgagga gctgcttgta ggtgacgtgg gtcgggtcgt atttgacttg caccacctct
    24121 gcgtggccgg tgccgccgga cgaaacctgt tgataggtgg ggtttggggt cgtgccgccc
    24181 atatagccgg gcgtcgtttc tacgacgccc ttgaccttgt cgaagtcgga ttccgtgcac
    24241 cagaagcagc cacttgcgaa tgtggcgatt tccagcttct ggccagattc cgcaggggcc
    24301 tgcgcggtct gcgcggcggc agccgttgcg aggaacagga gagcgccgac tgcgcgtgtg
    24361 atcaggtgaa tcatggatcg acgctcccgc acggacgtat cgccctcaac tcgaggtttc
    24421 agccttaggc acgaatttca aagccacgcc attcatgcag tagcgcaggc ccgtcggcgg
    24481 cgggccatcg tcgaaaacat ggccgagatg cccgccacag cggctgcaat gcacctcagt
    24541 gcgtgtcatg aaaaacttgc gatccacgct ggtgccgatc gcaccgtcga tcggcttcca
    24601 gaaactcggc cagccagtgc cgctgtcaaa cttggtctcc gacgagaaca gcggcagatc
    24661 gcatccggcg cagtggtaag tcccgggtgc atagttcttg tcgagcggac tcgagaaggc
    24721 atactcggtc ccgtgctcgc gcagaacatg gtactgctcc ggcgtgagga tcttcttcca
    24781 ctcctcgtcc gtcttggtga tctcgaaggt ttcctgctcg gacttgccgg ccgagaagcc
    24841 gcgggtggcg gtgaacccgc caagcagggc ggctgcagcc ccgatcattc caaattggcg
    24901 acggctcatc atgtctgggc tccttggatc cctgagattt ttcgcatcgc taccgacgct
    24961 tactgagaga tatgcgccga cacgcccggg ccgtaaagtc gggcgcgatc aaactctcgt
    25021 gcgcaccttc gggccgcggc gcctcgcccc gccaacctct atgccctcga tcggaaccgc
    25081 agtgtcgacg acgatctcct cggggctgag ccggcagcgg taggcgagca tctcgacacc
    25141 ggcggccctg gcctcgtcga aagcctcccc gtaggccggg tcgatgtcgc gcgcgaggtc
    25201 gagctgcagg gcatcgcccc gctggatcag gaacatcatg accgcgcggt gcccactgcg
    25261 caccatgtcg gcgagctcga gcaggtgctt ggcgccccgt tgcgtaacgc tgtccggaaa
    25321 ctcggcgagc cctgcccggc gcatcatgtg cacgttcttg atctcgatgt agcagcggcc
    25381 ctttttggcg caatcgagca ggatgtcgat ccggctgttc tggccgtact tcacctcccg
    25441 ccgcaaatcc ggatagccgt cgagcccggc aacgagaccc tcgccgatgg cctccgccac
    25501 gagcgcattc ggccgcgagg tgttgatgcc gaccagcgtc ggcccctgcc caaggtcagc
    25561 ctcgaccatc tcccaggtgt gccggtactt gcgcgtcggg ctgtcgcttt ccgacagcca
    25621 cacgaccgag ccaggctccg tcagcccaat catcgagccc gtgttcgggc aggtggccgt
    25681 gaccgtttcg ccggagtcca gcacaacgtc ggccagaaag cgcttgtacc gctgaacgag
    25741 gcggccgcgg atcagaggag cagaaaaacg catggcgggg aaactatggc ggccctatgg
    25801 ggcagcgcaa gcctgggtgt aagctggcgt tgaaaactca gcgcgaggta catggggatg
    25861 gcactggttc aggacaacga catcgtcacg gccgcggtgc tcgtcatcgg cgacgagatc
    25921 ctgtcggggc gcaccaagga caagaacatc ggctacatcg ccgagcattg cacagcgatc
    25981 ggcatacagg tgcgcgaggt gcgtatcgtg ccggatgtcg aggatgagat cgtggctgcc
    26041 gtcaatgctc tccgagctcg gtatgactac gtgttcacga ccggcggcat cggcccgacg
    26101 cacgacgaca tcacggccga cgcgatcgcc aaggcgttcg gggtgaagat cgacgtcgac
    26161 ccgcgggcgc tggcgctgct tctgccctac tacgagcggc gcggggtcga ggtcacccca
    26221 ggtcgcctgc gcatggcccg cattcccgaa ggggccgacc tgatcgagaa ttccatctcc
    26281 gttgcgcctg gcttcatgct gggtaacgtc atcacgctcg ccggcattcc caacgtcatg
    26341 caggtgatgc tcgacgccgt gacgccgcgg ctgcgcaccg gcaggaagat gcattccgtc
    26401 tccatcgatc tccaggcgcc ggaagggact attgccgggc tgttcggcga gcaccagaag
    26461 gcctatccat ccgtgtcgat gggcagctat ccgacattca aggataaccg accgagcacg
    26521 cagctcgttc tgcgatcaac cgatcccgac ctgctggccg aagcggccga cgggctccgc
    26581 cagaagcttg cagaacacgg ttttctttaa gacccgtgtt ggagttgcgg acgtcaggcc
    26641 gtccgcagca gctccgccac cgcttccgtg aacacctgtg cgccggtgat gatgtcctcc
    26701 tccttcgtgt cctcggccca gtggtgggag atgccgccga tggaagggac gaacagcatg
    26761 ccggcaggca tgtggcgtgc aaaaacctga gcgtcgtggg aggcaccact tggcatgcgc
    26821 atgtgcttgc ccggtgcgat gcgttcggcg gccttttcca gagccgactg cacgcgcgcg
    26881 tccattacgg ccggcgtgcc tttgcgcaac gcctcgacgg acgcgcggca cggaccggca
    26941 gcatccgatg cggagaccag atcgaaaagc gcacgctcca tgcgctccag gatcgcggta
    27001 tcggcgtcgc ggatctggaa cagcatctcg gcgcgaccgg ggataatgct cgctgccccc
    27061 gggtgcaatg tgatgcgacc cgtcgtccag acgctccgag gtccggcaac ttcaggcagc
    27121 cggcgctcga tctcgcagca caagcgggtg agcgccacgc cggcgtcctt gcggatggcc
    27181 atgcgtgtcg tgccggcgtg gttcgttacg ccttcgaatg tcacccggta ctgccatatc
    27241 ccgacgatgc tcgtgacgat gcccaggttc agatcgctcg attccagaac gtcgccctgc
    27301 tcgatgtggg cctcgacata ggcgatgtag tccttcggat cgcacacgat gcgcggccgg
    27361 cccgccaggc cggcgctctc gagggcatcg cgcagcttga cgccgcgcgt tggatcgaaa
    27421 gcctcgtcga tctcctgctc ggtgaggtcg ccgcagaagg accggctgcc aatgaggtta
    27481 ccgaaatgac cctcctcgtc tgcccacgcc cctacgtcca cgaccttgcc ggcaaaatcc
    27541 ggatcggccc cgatcacacg tgcggcctcg agcccgtaga tcaggccaag cggcccatcg
    27601 agccagccgg catagttctg gctttccgtg tgagaccccg tcaggatacg cgggccgcta
    27661 cccttcccgc ggccaatgac gttgccaata ccgtcgatct cgggctcgag cccggcatcg
    27721 gccatccggc cggcaagcca atgccgcgcc gccatgtcct cgcgagagta ggtcggccgg
    27781 tggacgccgg tcttgtactt gccgaattct gcaaagcgcc tgaggtctcc catgacgcga
    27841 tcgggatcga tggcgggcat atcggtgtcc gtggttgaag aggtcgggat gggccgcgaa
    27901 gatggcaggc gcctatcgta ggacagatcc cgaagaactc aatggaaagt aacggtggga
    27961 atcgttcgag aacggatcgg gcgcccccgc cagttatttg catccgaggg gtggcacgga
    28021 acccggttcg ccggtagcga tctgagtgcc gctcatcgtg gttttcctgg cccaggcggg
    28081 cgggcattcg ggcgtccatc ggctgtcccc tgccgcatgg gtgctcccgt aagaaagtcg
    28141 atgagccctc gccagagacg gcgtggcggg gaccgcactc gcgattgcga gtccaacggt
    28201 taacagacca gcgagcagag ctttcgtcac ggcggcgcgc gcgcactccc gacggagcgt
    28261 ccttcattcc agaataccca tcaacctgat gaaagatcgt gggttccaga gacctactga
    28321 cgcgccggcc tgttaacctt atccaggtca ccaagccggc ttctcagcgc ctcagatgca
    28381 aaaccacgtg gcgcacggtc ggatcatcct tctccggctc gatggagaaa ccgagctccg
    28441 cgaccatctt gagcatggtc gtgttctcgg cgagcacgga accgaagagc tgctctagac
    28501 cttcggcgcg agcgtaggcg atgaggtgct gcatcagctc ccagccaagc ccgcggccct
    28561 tgagatccga cctgacgagc accgcgtatt ccccttttgt gtaatcgggg tcggcgatga
    28621 agcgggacac gcccagcagc tcgcccgtct tcttcgacag cgcgacgaat gccatttcgc
    28681 gcgcgtagtc gatctgcgtg agccgcgcca ggaatttgcg cgagaggtcg gggcgggcag
    28741 tgaagaagcg cagccgctgg tcttctggcg tgaggcgcgg gaagaagtcg gcatagagcg
    28801 gctcgtcctc cggccggatc gggcgcaggc gaatttcccc tatgcccttt agctcgatgc
    28861 ccttgaccca ttccgacggg tagggccgga gcgccatggg aacgcggggg tttttccgtt
    28921 cgtcctccac cttcacccgg gcatccagcg cgatcacgcc cgattcgtcc gcaagcagcg
    28981 ggttgatgtc gatctcgcgg atctcgggat ggcgcgctac cagatagctg aggcgtacga
    29041 gcgactcggc aattcggtcg agatcggcgg caggacggtc gcgatacccg cgcagcagac
    29101 ggaagatgcg cgtccgctcc atcaggtctc gggcaagatt catgtcgagc ggcggcaggg
    29161 cctgcgccgt atcgttcacc acctcgaccg aggtgccgcc ggcaccgaac aggagaaccg
    29221 gcccgaacgt ccggtcgacg gccatgccgg cgatcagctc gtgcgcgcgg ggccgccgaa
    29281 tcatcggctg cacggtgaaa ccctgaacgc gcgcgtcggg caactggcgg gcgacgcggg
    29341 cgaggatgtc ctgtgcggca tcgcgtgcag cagtcgcccg ttcgaggccg aggcgcaccc
    29401 cgccgacgtc cgacttatga gagatgtcgt ccgagagcac cttgaccacg caggccgcat
    29461 gctcctgcag aatccgagat gcgatcgccg ccacttcctc ggggtccctg gcaacttcgg
    29521 tcgggaccgt cgggatacca taggcggcga gcagcgattt tccctccact tccgacagca
    29581 cgtggcgctc ctgccggagc gcgtcggcga tgatcgtttc ggcctcgtgc gtccgcagcc
    29641 cgagctcctc cggcagcgaa ggcggcgtgc gcatgagctg ggcctgcgca cgcgtgtatc
    29701 gcacgagctg catgaatcct tcgatggacg tgttcgggaa ttcgaagctg gcaatctggt
    29761 gctcggcgaa aagctgccgc gcctcccggc tggcgccctc tccgagccag ttggtgaaga
    29821 tcggtttcag cggctgcttg gcctgtcggc gcttttcgat gaggttcacg ataccttctg
    29881 caaccgccgt gctggacgcg gcagccgtcg ggcagttcat gatcagaacc gcatcggtat
    29941 ctttctcctc gagcagcact tcgagcgcgg ccgtgtagcg ctccgggccg gcatcgccaa
    30001 tgatgtcgac cggattgccc ttcgaccacg ttggcggcag caccgcgtcg agtgccgcct
    30061 ttccggcatc cgaaagctcc gccagcgtcc cctgcaaatc catcaggcga tcgaccgcca
    30121 gcaccgcgac accgccgccg ttggtgagga tcgccaatcg ctcgcccgtc gacgcaggca
    30181 gccgcgccag actctcggct gccccgaaca gctcgtagag cgctaagaca cgcagcacac
    30241 ccgcgcgcct gaaagcagca tcataggcgc ggtcggagcc ggtcaatgct ccggtatgcg
    30301 acaatgcagc cttcgacccg gcctcgtggc gaccagcttt caccacgatc accggcttgg
    30361 accgggctgc acgccgcgcc gctgacagga acttcggcgc gtgggtgacg gattccatgt
    30421 agagcaggat tgcccggctc ttcgggtcgc cggcgagata gtccagcatg tccccgaaat
    30481 cgacatcggc catgtcgccc agcgagacca catgggaaaa accgatttgc cggtcgatgg
    30541 tccaatcgat cacagccgtc gccagcgcgc ccgattggga cagaaaggcg aggtcgccgg
    30601 ccagcggtgc gcgatgcgaa aagctggcgt tgagaccgat ccccggcacc atcagcccga
    30661 tgcagttcgg accttggacg cgcagcaggt aaggcttcgc cgcattcaac atcgcctgcc
    30721 gcagttcgtc ccgaacgccg gctgtaatga cgacagcagc gcgcgtgcct tttcggccta
    30781 gctcgtcgat gatccccggc acggtcggcg gcggcgtcgc gatgacggcc aggtcagggg
    30841 tatcgggcaa atcttcgatc tttgcgtagc aaggcacgcc ctccacgtcg gagcggtgcg
    30901 gattgacaag ccaaatcggg ccatcgaagc cgccggcccg gaggttctgc gtaacgatga
    30961 ggccgacact gccgggctgc ggactggcgc cgatgaacgc caccgacttg ggcgccagga
    31021 ggtgctgcag gttgcggatg gtcatttgtc aggtctcttg tttcattgag ccgtcggtta
    31081 ctcaccctgg ccggagagcg ccttttgcgc ccgccgcact gtctcctcgc gatcaccccc
    31141 tgcgtcgacg actgtccatg acggcgaaag caatccgata tcgtaggaaa tctggctttc
    31201 gaccacgtca acggtggcgt cggaggcatc gttcctgcgg gcagcgacgc gcgccatcag
    31261 gacctcgggc gacgcggtca gccaaagccc cttcaaccgc aggccggcct tttctgcttc
    31321 tgcctcgagg cggagccgtt catcggctcg cgcgaacacc gcatcgacga tcacgccatg
    31381 acccaccgcg agaacgcgcc cggtcttctc caccagggtg gcataaacac gagcggtgac
    31441 ttcctccgtg tagccttcgg cgccgagtct ctccgtctcc gcccggccga acagcgcctt
    31501 gcgctccaga tcgctcctta agtggactgc gccgggacag ggcccgatcc ctggcgcaag
    31561 cgcactggcc agcgtcgact tgcccgtgcc cgaaagtccc ccgacggcaa cgatctttgg
    31621 aggcgcggga tcgagatggc tgagagcagc ttcgatatac cgccgtgccc gctcgacgga
    31681 ttgcgccttg cgacctgatg ccttttcctg ctcagcccgt tcggccgcca cgagtgcgcg
    31741 aattccggcc cggagcccga ggaacaacgg cagtgccgcc agaccatcga ggtcgagcat
    31801 ggcctgcgat cgccagagat agcggttgag caccgtgttg gccgccctcc gcagaccgcg
    31861 aaaatcgaga tccatcagca ggaaggcgag gtcgtagagg gtatcgatgg tggcgagctc
    31921 ctcgtcgaac tcgatggcat cgaacagcgt cgggcggcct ttccacatga cgatgttgcc
    31981 gaggtggagg tcggcgtggc agcgccggac aaatccggat tcggcgcgcc gatcgagaac
    32041 cgttgcagcg cgggcgagct gcgcctcggc gcgcttgcgg aaacgcgtcg cctcatcctg
    32101 tccgatgacg tttgcgacac ccgcgagatt gcctgaaacg ctgtcgacca ggctggccag
    32161 acgcgcggcg ccgagaggat cgttcacccg gggcgccttc tcgtgacttt cgaacgccgc
    32221 gtctgccaga gcccgggcga ggccttggct gatgccctcg cgatccgcga tgctgccgag
    32281 aaggtccgac tgggggaaac ggcgcatccg cacggcccat tcgacgggct cgccggttcc
    32341 gcccaacgcc agccggccgt ccggctcccg ggtgatcgga atgcaaccca agtagatttc
    32401 cggcgcccag atgcggttca cctcgacttc gcgctcacag gtggcccgcc gcttctccag
    32461 cgtcgacagg tccatgtagg gaaaccgaac cgcgcgcttg atcttccaca cctgatcgcc
    32521 ggccaggaag accatcgcgc catgggtctc gatccgctct accggtgcgg cggcagaccc
    32581 gccataggtg gccggatcgg caaggaagcg cgcaacctcg gactgatccg tgtctgcaac
    32641 cccgatcctt gcggccatcc tgtagacctc gatctgaaat ccgggcgatt gagcgctgtc
    32701 tccagccttt caatcgcttg cgtccatgcc cggcaaccaa gtcgacggtt tttgacgagc
    32761 gatcggccgc gcgcgctatc attgctttcg atcccagggc ttccttgacg cctatcaaag
    32821 ctgcggcagc gctggtccgt atgctcagct taagcgttca aacggcgttg gataccctcg
    32881 atctccaata acgggagctc tctcgatggc ttacaaaaca attctgatgc acctcaatca
    32941 tgatcggcgg gccaagcagc tgttgggcgc tggcgtgcag ctggcgagag ctttcgatgc
    33001 gcatctcatc ggcctgcatg tttttcctgc ctaccgcttg aaccctccgg tgccgctgcc
    33061 gatcggccag gacatcctcg gccgcatcgt ggcccagatc gaggaagaag cgaaccgcat
    33121 aaagtcccag tttgacgacg ctacgagcct gcaaaccttc gtttcggagt ggcggtccct
    33181 aaggtgcgag cgaaccgatc cggcagaggt cgtcatgcgc catggctacg ccgccgacct
    33241 gctgatcgcc agccagtcgg atcccaactg ggaaatgacg tcggtcctcg acttccccga
    33301 ccggctcgcc atcgagtccg gccgaccggt gctcgtcatc ccggacggct acgttttctc
    33361 cgaaccgccc aagtctatca tagttgcctg gaaggatcgc cgcgaagccg cccgcgccgc
    33421 attcgacgcg cttcccctct tgaaggtagc ccaaaccgtt gaggtgctga ctgtcgagga
    33481 ggaagagcat cgcgagggtg gaccggagga tatcgagatc gccactgcgc tcgaccggca
    33541 cggcgtgaag gtttccgtca cgaccatgcc ggcaagcaga actcctgcct cggaggaaat
    33601 cctcaaccga gcggcccaga attcacagct cctggtcatg ggcgcctacg gccactcccg
    33661 cttccgcgag ttcgccttcg gcggcgtgac ccgtcatatc ctgaagaata tgacagtacc
    33721 ggttctgttc tcccattgac cctcgaacgg gaggaggctg ccccatgaaa aacccagaaa
    33781 agctcttgcg ccgtgtgcgc ctggagcttg caagagaccc ggactttccg gctggcagcc
    33841 gcgagcgggg ctacgacttc atcgccccca tcgacgagaa gggccagatc gttcattcgg
    33901 cctgggagca attgaaggac cgctgccgcg tgcggcggtt ctggggaacc gaacccgatg
    33961 aggtcggcca tatcgtccac agtcccggcg gcgagtggcg gtttcactac gatatacgcg
    34021 gtgataagcg tcacgacgag gccggttacc gcttctcacg ccactccttt ttgccaggtg
    34081 agtatgtctc catcctcgag caggatggtg tgatgcgcac attcttcgtt gcatcggtgc
    34141 gcgaaatcga ttgaatgaac cggtttcacc cgaggc
//
LOCUS       DASVDU010000148        11479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_818682, whole genome shotgun sequence.
ACCESSION   DASVDU010000148 DASVDU010000000
VERSION     DASVDU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818682"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1665)
                     /gene="glmM"
                     /locus_tag="VF226_09460"
     CDS             complement(310..1665)
                     /gene="glmM"
                     /locus_tag="VF226_09460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7074254.1"
                     /translation="MSRRYFGTDGIRGLANRDPMTSEIALKVGMAAGRIFADGKQRIR
                     VVIGKDTRLSGYMIESALMSGFTAVGSDVFLLGPMPTPAVAMLTRSLRADLGVMISAS
                     HNPYRDNGIKLFDSEGYKLSDEMEAKIEELILADPESLLAEPDAIGRATRVESAQERY
                     IEFAKRTLARDLRLDGLRVVIDCANGAGYKVAPEALWELGAEVIKIGVDPNGRNINHK
                     CGSTAPDALIDKVKELRADIGIALDGDADRLVIVDEKGQLVDGDQLMAVIAESWLKAG
                     RLAGGGIVATVMSNLGLERYLAGLGLSMIRTPVGDRYVVEHMRKHGFNVGGEQSGHIV
                     LSDFATTGDGLISALQILAAVVSAGKPVSEVCRRFEPLPQRLQNVRYANGMPLDNERV
                     RQAIEEGKARLGTAGRLVIRPSGTEPVIRVMAEGDDERLVAAVVRDIVEAVREAAVPA
                     E"
     gene            complement(1885..3690)
                     /locus_tag="VF226_09465"
     CDS             complement(1885..3690)
                     /locus_tag="VF226_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7074255.1"
                     /translation="MLRFECRSTRAEAVFTLSPKLLAIGLGIALAACSADISRLDPPF
                     GLTTSSSRISSTPVIPPEGMLGGGSRLADSTPYDTYSSTPYDTYRPAPRNDYATAPRT
                     YDRGGSEYRPSYGTNPSYRGSYGGYDAGRRVASAPPSSDSRTAYQPSYPARPSSTYQP
                     PTAYEGQRPSYAAQPRPQSEVAALSPEATQQPYRTVPGPDSTKRATAPYAPQPATSRP
                     NPSSNPRLIDVQPGDTLIGIADRYNVSISELMSINGLSSPVLQPGKRLLLPQNAKLPV
                     ATARHTTPGPEAKQVAALNDRGPTVAPVSVSDARPRDEAPIRYSDDLDAEESSAASGT
                     HTIQKGESLYRIAVRYGVPLAELQRINGISDPRKVRVGTVLKLPTASGANPSAVASGT
                     AEPASAKAQPSERTRPATTPVAIDAVGEAPSKPVIINARTKIAKLEDGKTANDAGPIT
                     AVPSVSADKSPADDSKASAEEQADGVGKFRWPVKGRIIAGFGKQADGSYSDGITLAVP
                     AGTDVHAAESGVVAYAGDELKDYGNLVLIRHDNGWITAYAHNSELQVKRGDRVRRGQV
                     IAKAGNTGNVDRPQLLFELRKGSSPVDPIPHMEKL"
     gene            complement(3869..4441)
                     /locus_tag="VF226_09470"
     CDS             complement(3869..4441)
                     /locus_tag="VF226_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEX7074256.1"
                     /translation="MKVLWGKIWPAIAVVAVLQTAVLGWMVAERVRLLQTGREVILPI
                     VPVDPRSLFRGDYVRLSYEVTRVPGHLLKGKATRGAPIYVTVQRQVDGSYVPVAASET
                     HPGVAAPDQIVLRGRVEHDGRRNDSGAGDLFLSYGIESYFVPEGKGLQLEKMAGERKL
                     AAVIAVDRHGNSAIKGLMIDGTLVYEEPLF"
     gene            complement(4438..5532)
                     /locus_tag="VF226_09475"
     CDS             complement(4438..5532)
                     /locus_tag="VF226_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEX7074257.1"
                     /translation="MWSYRARLERDLTRWREQGWIDDAGLNGIRADLAERGRGVGLST
                     TLAVLGAVLIGFAVMSFVAANWQEMPRLARLGLLVAALWGCYGLAGHLLIRGLEGFGH
                     AAVLAAVGVFGASIMLIAQMYHIDGNPPDAVLTWAAGALLAGVLLRSNPALALAMLLI
                     GLWSAWETVLTQAVHWPFLIGWGAVSAAFLWRRWRPGLHLSAILMSIWIIALGYLLFE
                     RHAHWAVALIGLGICGVAVAAEQLPGVWRRIAPAILCYGMAIAYGGLFAFKFLEQRDA
                     GTLTLLSVVTMSLLLAAIFWGWRGDHKPVLWLGYLGFSVELLALYFVTIGTLLGTSLF
                     FLVTGIIVIGLAWLAYRLHTQRPGTEEALP"
     gene            complement(5617..6168)
                     /gene="ilvN"
                     /locus_tag="VF226_09480"
     CDS             complement(5617..6168)
                     /gene="ilvN"
                     /locus_tag="VF226_09480"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX7074258.1"
                     /translation="MPQTQKQSHYPDPVKKEEIVSRTLSVLVDNEPGVLSRIAGLFSG
                     RGYNIDSLTVTETEHASHVSRFTIVTKGTPMVIEQIKHQLERMVPVHRVTDLTNLSEP
                     PLERELALIKVAGKGEKRIEALRLAGIFRANVIDASTEHFVFELTGRTSKIEQFIQIM
                     TELGLVEVSRTGIAAMARGPEGL"
     gene            complement(6187..6870)
                     /locus_tag="VF226_09485"
     CDS             complement(6187..6870)
                     /locus_tag="VF226_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7074259.1"
                     /translation="MHLTIANKLYSSWSLRPWLVLKAFGIPFEETVIPMYQPDSKARM
                     LAVSPTGKVPLLTDGKIKVWESLAIIEYIAEKFPQAAIWPRDVEARAHGRSAANEMHA
                     GFQALRHACPMNLAKRFAKKDRGPEVAANVARLQALWREARTAFGSGGPFLYGGFSAA
                     DAMFAPVVTRLDTYQVDVEADTRAYMDAVLSHPAFIAWREAALEEPWHLPHYEEGETA
                     VEIFHRPQS"
     gene            complement(6902..7300)
                     /locus_tag="VF226_09490"
     CDS             complement(6902..7300)
                     /locus_tag="VF226_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7074260.1"
                     /translation="MIAIDTNVLVRFVVADHPEQFERAMVLIRDNTIFVGLTVFLEAE
                     WVLRSVYGATREEIATAFLGVAGLPNVTVESPAVLIEAVAAYRKGMDFADALHVAAAA
                     ESNVAEFATFDEDLGKRSRSALRHVRVLNI"
     gene            complement(7297..7566)
                     /locus_tag="VF226_09495"
     CDS             complement(7297..7566)
                     /locus_tag="VF226_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018899306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7074261.1"
                     /translation="METTRLSSKGQVIIPKSVRDARGWEPGTELIVEDHPEGVLLRSH
                     RVFAPTRFEEVAGCLKRKGRPRTLQEMDKAIEAEARLRWKKFEAQ"
     gene            complement(7647..9413)
                     /locus_tag="VF226_09500"
     CDS             complement(7647..9413)
                     /locus_tag="VF226_09500"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEX7074262.1"
                     /translation="MARPMTGAEMVITALQDQGVEYIFGYPGGAVLPIYDELFQQDKV
                     KHVLVRQEGGAVHAAEGYARSTGRVGVVLVTSGPGATNAVTGLTDALMDSIPVVCITG
                     QVPTHLIGSDAFQECDTVGITRSCTKHNWLVKDVNDLGRILHEAFYVARTGRPGPVVI
                     DIPKDVQFATGNYVGPSNIAHKTYRPRLKPDIERVREAVELMATAKRPIFYTGGGVIN
                     SGPKASQLLRELVRLTGFPITSTLMGLGAYPASDKQWLGMLGMHGTYESNMAMHDCDV
                     MIAIGARFDDRITGRIDAFSPNSKKIHIDIDPSSINKNVLAHVPILGDVAYVLEEMLN
                     LWKSRAPQLDKAAHRAWWKQIAQWRERNSLSYKQSSDFIMPQYAIERLYALTKDRDTY
                     ITTEVGQHQMWAAQFYRFEEPNRWMTSGGLGTMGYGLPAAIGTQIAHPESLVIDIAGD
                     ASILMNIQEMSTAIQYRLPVKVFILNNQYMGMVRQWQQLLHGNRLSESYTEALPDFVK
                     LAEAYGGVGIRCDKPADLDDAILEMIRTPKPVLFDCRVAALANCFPMIPSGKAHNEML
                     LGQDVTDEEIGSAIGKEGKVLV"
     gene            complement(9530..10465)
                     /gene="miaA"
                     /locus_tag="VF226_09505"
     CDS             complement(9530..10465)
                     /gene="miaA"
                     /locus_tag="VF226_09505"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX7074263.1"
                     /translation="MNSLNSCRAILIAGPTASGKSALALALAERYGGVVINADSMQVY
                     RELRVLTARPTPEEEARVPHALYGFVPAREAYSAGRYMKDAARAIAQAEAQGRLPIFV
                     GGTGLYFKALLEGLSPIPPVSAEIRTYWRAQAQRIGAQALHQVLSERDPVMAARLRPS
                     DPQRIVRALEVLDSTGRSLAEWQSLPGEGVLREDETVRLVLLPDRATLYRRCEERFDL
                     MLRQGALDEVAALREQQLDPELPVMTAHGVPHLLAALAGDIDLETAAARAKQDTRHYA
                     KRQFTWIRRNMQSWKHIDAQQMESLCSKLERLLKL"
     gene            10521..11429
                     /gene="serB"
                     /locus_tag="VF226_09510"
     CDS             10521..11429
                     /gene="serB"
                     /locus_tag="VF226_09510"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX7074264.1"
                     /translation="MPDRYALVLTSSPAAPALGRDAVDRAVSAFPDGLSPDPGWLAEG
                     EAWQAAFVAKGPGEPERVRSAVAAALTDFPIDVNVVANDAARRKKLLVADMESTIIEQ
                     ECLDELADHIGLRDRISEITARAMRGEIAFEEALKERVGLLKGLDADVLQKLYDRITL
                     VPGARELVATMRKNGAYCALVSGGFTFFTERIAKRLGFDTHQANRLAIVDGKLAGTVA
                     SPILGREAKLAALKRLTQELGLDPQETLAVGDGANDLDMIRAAGLGVAFRAKPITAAE
                     AKAAIRHGDLTALLYLQGYRKDEFVR"
BASE COUNT         2035 a         3770 c         3614 g         2060 t
ORIGIN      
        1 atcagcgcca ggcggcgcac tttcctgaac atatgcaatg ctcccccact gcagacggcg
       61 ctctcgctcg agcgccgccc tccccctcat acgcacgcat acggatactg gccggaccgt
      121 gcggcccgga gacgctgctt aggcgcacac gctcgcgatc ccggcaactt gcggggcgcg
      181 agcggaaagg acgcgtactg gcgtgatgcg cgctcgtggc ggcatcagtg cgcgagctgt
      241 ctcagaccgg gattgtgagc agaaaaaatt taagggagct taattacgct ttcccgcaac
      301 cggaaacgat cattcggccg gcaccgctgc ctcgcgcacg gcctcgacga tatccctcac
      361 cacggcggcc acgagccgtt catcgtcgcc ctcggccatg acgcggatca cgggctccgt
      421 tccggacggc ctgatgacaa ggcggccggc ggtgcccagt cgcgccttgc cctcctcgat
      481 cgcctgccgg acacgctcgt tgtcgagcgg cattccgttc gcatagcgca cgttctgcag
      541 ccgctgcggc aacggctcga accgccggca cacctcgctg accggcttgc cggccgacac
      601 caccgccgcc aggatctgca gagcagaaat gaggccgtcg cccgtggtcg caaagtccga
      661 caaaacgatg tgccccgact gctcgccgcc tacgttgaag ccgtgcttgc gcatgtgctc
      721 gacgacatag cggtcaccga cgggcgtgcg gatcatggac aggccgagcc cagcgagata
      781 gcgctccagc ccgaggttcg acatgacggt cgcgacgatg ccgcctccgg cgagccgccc
      841 tgccttcagc cagctctccg cgatgacggc catcaactgg tcgccatcca cgagctggcc
      901 tttttcatcg acaatgacga gccggtcggc atcaccatcg agcgcgatgc cgatatccgc
      961 gcgcagctcc ttgaccttgt cgatgagagc gtcgggcgcc gtcgacccgc atttgtgatt
     1021 gatgttccgg ccgttcgggt cgacgccgat cttgatcacc tcggcgccaa gctcccagag
     1081 cgcttccgga gccaccttgt agccggcgcc gttggcgcaa tcgatcacga cgcgcaggcc
     1141 gtcgaggcgc aggtcgcgcg ccagcgtccg cttggcgaat tcgatgtagc gctcctgcgc
     1201 gctctcgacc cgtgtcgccc ggccgatggc gtcgggctcg gccagcaggc tttcgggatc
     1261 ggcgaggatc agctcctcga tcttggcctc catctcgtcg gacagcttgt agccttccga
     1321 gtcgaagagc ttgatgccgt tgtcgcggta gggattgtgc gaggccgaga tcatcacgcc
     1381 gaggtcagcc cgcagcgagc gcgtcagcat ggcgacagcc ggggtcggca tcggaccgag
     1441 caggaacacg tcggagccga ccgcggtaaa gcccgacatc agcgccgact cgatcatgta
     1501 gcccgacagt cgtgtgtcct tgccgatcac cacgcggatg cgctgcttgc catcggcgaa
     1561 aatccgtccc gccgccatgc ctaccttgag cgcgatctcc gaggtcatcg gatcgcggtt
     1621 ggcgagaccg cgaatgccgt ctgtgccgaa gtaccgacgt gacatctctc cgctcagctc
     1681 ctgcaattcc cagcacctgc atcgcgccca actgtggcgg aggtgtgctc cgccggcccg
     1741 ggcgcagttt ccgatccccg gaggtttata gcacccccta aacgggacgc agttgcaacg
     1801 cggaacatcg tcaacaagat gcgccggccc gtgggggagc gggccggcgc tcggaagctg
     1861 gcaaggttcg gaggctccct tgctctacag cttctccatg tgagggatgg gatcgaccgg
     1921 gctcgagccc ttccgcagct cgaaaagaag ctggggcctg tcaacattgc ccgtgttgcc
     1981 cgccttcgcg atcacctgac cgcggcgcac gcgatctccg cgcttgacct gcagctcgct
     2041 gttgtgagcg taggcggtga tccagccgtt gtcgtgccgg atcagcacga ggttgccgta
     2101 gtccttcagc tcatccccgg catacgcaac gaccccgctt tcggctgcat ggacgtccgt
     2161 tccggccgga acggcgagcg tgatgccgtc gctgtagctg ccatcggcct gcttgccgaa
     2221 cccggcgatg atccgcccct ttacgggcca acggaacttg ccgacgccgt cggcctgctc
     2281 ctcggcgctc gccttgctgt catcggctgg cgatttgtcg gcggaaacgg acggcactgc
     2341 cgtgatgggg cccgcatcgt ttgccgtttt gccatcctcg agcttcgcga tcttggttcg
     2401 ggcgttgatg atcactggct tggacggggc ttcaccgacg gcatcgatcg cgacaggcgt
     2461 ggtcgcgggc cgggtccgct cggacggctg cgccttagcc gaagcaggtt ccgcggttcc
     2521 cgacgccact gcggatggat tggctccgga ggcggtcggc agcttcaaca cagtgcccac
     2581 gcgcactttc cggggatcgc tgatcccgtt gatgcgctgc aactcggcca gaggtacgcc
     2641 gtagcgtacg gcgatccgat aaagactctc gcccttttgg atcgtatgcg tgccggaggc
     2701 ggctgacgat tcctcggcgt ccagatcatc ggaatacctg atcggcgcct cgtctcgcgg
     2761 acgtgcatcc gaaaccgaaa ccggcgccac cgtcgggccg cgatcgttga gtgcggcgac
     2821 ctgcttggcc tccggcccgg gcgtcgtatg acgggcagtc gcgacgggaa gcttggcatt
     2881 ttggggcagc agaagccgct tgccgggctg cagcaccggg ctgctcagcc cgttgatgct
     2941 catcagctcg ctgatcgaga cgttgtagcg atccgcaatg ccgatgaggg tatcgcctgg
     3001 ctgcacatca atcagacgcg ggttgctcga aggatttggc cgagacgtcg ccggttgagg
     3061 agcatacgga gcagtagctc tcttggtgct gtcgggcccc ggcactgttc tatacggctg
     3121 ctgcgtcgcc tcgggcgaca aggcagctac ctccgattgc gggcgcggct gcgcagcata
     3181 cgagggccgc tggccctcgt atgccgtcgg cggctgatac gtcgagctcg gccgggcggg
     3241 gtaggatggt tggtacgccg tcctcgaatc ggagctgggc ggagcagagg caacacgccg
     3301 gccggcatca tagcctccat agctcccccg atagctcggg ttcgttccgt agctcggccg
     3361 gtattctgaa ccaccgcggt cgtaagtgcg cggcgcggtg gcgtagtcgt ttcgcggcgc
     3421 cggccgatag gtgtcgtacg gcgtggaaga ataggtgtcg tacggcgtag aatcagcaag
     3481 ccgggagccg ccgcccaaca tgccttcggg agggatgacc ggagtcgacg aaattcggga
     3541 gctgctagtg gtcagaccga acggcggatc gagccggctg atatccgcgc tgcaggcggc
     3601 cagtgcgatc ccgaggccga tggcgagcag tttcggagag agtgtaaaga cagcttcggc
     3661 ccgcgtggac cggcactcga aacgcaacat gaaaacacac ctcgtttacc tactccctct
     3721 taaccacacg tgggttaacg gaggattaac gaggcgtgct catgctgctg cttgctgacc
     3781 cgtcaacaaa caaattcgta gcgaggacaa aaggttaggc ggtcagcagc ggttgccatt
     3841 tttgagggcg agcccacacc aaaccgaatt agaacagcgg ctcctcgtaa accaatgtcc
     3901 cgtcgatcat caggcccttg atggccgagt tgccgtgcct gtcgacggcg atgacagctg
     3961 caagcttccg ttcacccgcc atcttctcga gctgcagacc cttgccttcg ggcacgaaat
     4021 agctctcgat cccgtagctc agaaacaggt cgccggctcc cgaatcgttg cgtctcccgt
     4081 cgtgctcgac cctgccgcgc aacacgatct gatccggcgc ggccacgccc gggtgcgttt
     4141 cgctggctgc caccgggacg taagatccat ccacctggcg ctggacggtg acgtagatgg
     4201 gcgcaccccg agtggccttg cctttcagca aatgcccagg cacgcgcgtc acctcgtacg
     4261 agagccggac gtaatcgccg cggaacaggc tccggggatc gacaggcacg atcggcagga
     4321 tgacctcgcg accggtttgc agaagccgga cccgctcggc caccatccag ccgagcacgg
     4381 ccgtctgcag cacggccacg acggcaatcg cgggccagat cttgccccac agcaccttca
     4441 tggcagagcc tcctccgtcc ccggccgctg ggtgtgcagc cgataggcca gccacgccag
     4501 cccgatgacg atgatgccgg tcaccaggaa gaacagcgac gttccgagca gcgtgccgat
     4561 ggtgacgaag tagagcgcca gcagctcgac cgagaagccg agatagccga gccacaacac
     4621 cggcttatgg tcaccccgcc agccccagaa aatggcggcg agcagaaggg acatggtgac
     4681 cacggagagc agcgtcagcg tgcccgcatc gcgttgctcg agaaacttga acgcgaacag
     4741 gcctccatag gcaatggcca tcccgtagca gaggatcgcc ggcgcaatgc ggcgccagac
     4801 gcccgggagc tgctcggctg cgacggccac gccgcaaatc cccaatccga tcagcgccac
     4861 agcccaatgg gcatggcgct cgaacagcag atagccgagc gcgatgatcc agatcgacat
     4921 caggattgcg gacaggtgca agcccggccg ccaccgccgc cacagaaagg ccgccgagac
     4981 ggcgccccag ccgatgagaa acggccaatg gactgcctgg gtcaggaccg tctcccaggc
     5041 actccagagg ccgatgagca gcattgccag agccaaggcg gggttggaac gcaggagcac
     5101 accggccagc agcgcgccgg cggcccaggt cagcaccgca tcgggcgggt tgccgtcgat
     5161 gtggtacatc tgagcgatca gcatgatgct cgcgccaaac acgccgacag ccgccagcac
     5221 ggctgcgtgc ccgaagccct caagcccgcg tatcagcaga tggcctgcga ggccatagca
     5281 gccccacagt gcggcaacga gcagccccag ccgggcgagg cgcggcatct cctgccagtt
     5341 cgccgccacg aagctcatga cggcgaaacc gatcagcacg gcaccgagga ccgcgagtgt
     5401 ggtggaaagg ccgacgccgc ggccgcgctc cgcaagatcc gccctgatgc cattaagacc
     5461 ggcatcgtca atccagcctt gctcccgcca tcgcgtaagg tcccgctcca gacgggcccg
     5521 atagctccac atctcgcgcg cctccccgat ggctgcgccg gctcgtgtct cgattccgag
     5581 gacgctagcc cgccgacgat atcagtcgcc ggagatttac agtccttcag gcccgcgcgc
     5641 catcgccgcg atgccggtcc gcgatacctc gaccagcccc agctcggtca tgatctggat
     5701 gaactgctcg atcttgctgg ttcgcccggt gagctcgaac acgaaatgct cggtcgacgc
     5761 atcgatcacg ttggctcgga aaattccagc cagccgcagg gcctcgatgc gcttctcgcc
     5821 cttgcctgcc accttgatga gcgccagctc gcgttcgagc ggcggctcgc tcaggtttgt
     5881 gagatccgta actcgatgaa ccggcaccat gcgctcgagc tgatgcttga tctgctcgat
     5941 gaccatcggg gtgcccttgg tgacgatcgt gaaacgcgac acgtgactgg cgtgctcggt
     6001 ctcggtcacc gtcagcgaat cgatgttgta accgcgcccg gaaaacagcc cggcgatgcg
     6061 cgacaggacg cccggctcgt tgtcgaccag gaccgagagc gtgcgcgaga cgatctcttc
     6121 ttttttgacg ggatcggggt agtgtgactg tttctgggtc tgcggcatgg atgtcctgct
     6181 agcctctcag ctctgcgggc gatggaatat ctcgacggcc gtctcgcctt cctcgtaatg
     6241 cggaagatgc cagggctcct cgagggccgc ttcgcgccag gcgatgaagg cagggtgtga
     6301 gagcacggca tccatgtagg cgcgcgtgtc ggcctccaca tcgacctgat aggtgtcgag
     6361 ccgtgtcacg accggcgcaa acatggcgtc ggctgccgag aatccgccgt acaggaacgg
     6421 gccgccgctg ccgaaggcgg tacgcgcctc cctccagagc gcttgcagcc gcgcgacgtt
     6481 ggcagctacc tctggcccac ggtctttctt ggcaaatcgc ttggccagat tcatcgggca
     6541 ggcgtggcgt agcgcctgga agccggcgtg catctcgttt gctgccgagc gcccgtgtgc
     6601 ccgcgcttcg acgtctcgcg gccaaatcgc tgcttggggg aatttctccg caatgtactc
     6661 gatgatggcg aggctttccc agacctttat cttgccgtcg gtgagcagcg gaaccttgcc
     6721 cgtcggcgat actgccagca tgcgcgcctt ggaatccggc tggtacatcg gaatcaccgt
     6781 ctcttcgaac gggatcccga acgccttcaa caccagccac ggccgcagcg accaggacga
     6841 gtagagcttg ttggcgatgg tcaggtgcat cggcgtgtgc ccccagtcgg gtctcaacca
     6901 atcagatatt cagaacacga acgtgccgca gcgcggatct cgaccgtttt ccaaggtctt
     6961 catcgaatgt cgcaaattca gcaacattgg actccgcggc agccgcgacg tgcaatgcgt
     7021 ccgcgaagtc catgcccttg cgataggctg cgaccgcctc gatgagaaca gctggcgact
     7081 cgacggtgac gttcggcagc ccagcgacac ccaggaacgc ggtggcgatc tcctcgcgtg
     7141 tcgctccata gacactgcgc agaacccatt ctgcttcaag aaaaacggtc aaaccgacaa
     7201 aaatggtgtt gtcccgtatc aggaccatcg cgcgctcgaa ttgctctgga tgatcggcga
     7261 cgacgaacct gacgaggaca ttcgtatcga tggcgatcat tgcgcttcga acttcttcca
     7321 gcgcaggcgt gcttcagcct cgatcgcttt gtccatttcc tgtagtgttc tcgggcgccc
     7381 cttccgcttc aagcagccgg cgacctcttc aaaccgagtt ggcgcaaaca cgcggtggga
     7441 ccggaggaga acgccttccg gatgatcttc tacgataagc tcggtccctg gctcccagcc
     7501 ccgcgcatcg cgtacggatt tcggaataat gacctggccc ttgctcgaca ggcgagtcgt
     7561 ttccatctcg cgctcctcat tgagtaagac gaggtaagac gtaggtgtgc gccgggatgc
     7621 cgtcaactgc accccggctc tcaagcctac accagcacct tgccttcctt gccgatggcg
     7681 gagccgattt cctcatcggt gacatcctgc ccgagcagca tctcgttgtg cgctttgccc
     7741 gaggggatca tcgggaagca gttggcgagc gctgccacac ggcaatcgaa caggacgggc
     7801 ttcggtgtcc ggatcatctc caggatcgcg tcgtcgagat cggctggctt gtcgcaacgg
     7861 atgccgacgc cgccgtaagc ctccgccagc ttgacgaaat cgggcagcgc ctcggtatag
     7921 ctctcactca gccggttccc gtgcaggagc tgctgccact gccgcaccat gcccatgtac
     7981 tgattgttga ggatgaacac cttcaccggc agccggtact ggatcgccgt cgacatctcc
     8041 tgaatgttca tcaggatcga agcgtcgccg gcgatgtcga tgacgagcga ttcaggatgc
     8101 gcgatctgcg tgccgatcgc cgccggcaac ccgtatccca tcgtgccgag accgccgctg
     8161 gtcatccagc ggttgggctc ctcgaaccga tagaactggg cggcccacat ctggtgctgg
     8221 ccgacttccg tcgtgatgta ggtgtcgcgg tccttcgtca gcgcatagag gcgctcgatc
     8281 gcgtattgcg gcatgatgaa gtcgctcgat tgcttgtagc tcagcgaatt gcgctcccgc
     8341 cattgcgcga tctgcttcca ccaggcccgg tgcgcggctt tgtcgagctg cggcgcgcgg
     8401 gatttccaga gattgagcat ctcctcgagc acgtaggcga catcacccag gatcggcaca
     8461 tgggcgagca cgttcttgtt gatcgaggac gggtcgatgt cgatgtgaat tttcttggag
     8521 ttcggcgaaa aggcgtcgat ccggccggtg atgcggtcgt cgaagcgggc gccaatggcg
     8581 atcatcacgt cgcaatcgtg catcgccatg ttggactcgt aggtgccgtg catgccgagc
     8641 atgccgagcc actgcttgtc cgaggccgga taggcgccga gccccatgag cgtcgaggtg
     8701 atcgggaatc cggtcagtcg cacgagctca cgcaggagct ggctcgcctt cgggccggag
     8761 ttgatgacgc cgccgcccgt gtagaagatc gggcgcttgg cggtcgccat aagctccacg
     8821 gcctcacgga cgcgttcgat gtcgggcttc aaccgtggcc tgtaggtctt gtgcgcgatg
     8881 ttggacggcc cgacgtagtt gccggtcgcg aactggacgt ccttcggaat atcgatgacg
     8941 accgggccgg gccggccggt ccttgcgacg taaaaggcct cgtgcaggat gcggccgagg
     9001 tcgttgacgt ccttcaccag ccagttgtgc ttggtgcagg accgggtgat gccgaccgta
     9061 tcgcactcct gaaaggcgtc ggagccgatg aggtgcgtcg ggacctgccc ggtgatgcag
     9121 acaacgggga tggaatccat cagcgcatca gtgagcccgg tgacggcgtt ggtcgcgccg
     9181 ggacccgagg tgaccagaac aacgccgacg cgccccgtcg agcgggcata accttccgcc
     9241 gcgtgcaccg cgccgccctc ctggcggacg agcacgtgct ttaccttgtc ctgctggaaa
     9301 agctcgtcgt agatcgggag tacggcgccg cccggatagc cgaagatgta ctcaactccc
     9361 tggtcctgaa gcgccgtaat gaccatttcg gcgcccgtca tcgggcgtgc catacctcta
     9421 tctccgcagt gcttgtttgg tggcgtgggt tggaaacagg cggccggaag gatctccccc
     9481 ctctagccgg cgcgatgaac ctaacgatcg ctcacccggc cgtcaatcgt cagagcttta
     9541 gcaatctttc caatttcgag caaagacttt ccatttgttg cgcatcgatg tgtttccaag
     9601 actgcatatt gcgtctgatc cacgtgaact gccgcttggc ataatgccgc gtatcctgtt
     9661 tggcgcgcgc cgcggccgtc tccagatcga tgtcaccggc cagcgccgca agcagatggg
     9721 gcacgccgtg cgcggtcatg accggcagct ccgggtcgag ttgctgctcc ctcaaggctg
     9781 caacctcgtc gagagcgcct tggcgcagca tcagatcgaa acgctcctca cagcggcggt
     9841 agagcgtggc acggtccggc agcagcacga ggcggacggt ctcatcctcg cggaggacac
     9901 cctcaccggg caggctttgc cattcggcca aggaccggcc ggtcgagtcc agcacttcca
     9961 gggcgcgcac aatccgctgg ggatcgctcg gccgcaggcg ggcggccatc accggatcgc
    10021 gctcgctcag cacctgatgc agcgcctggg ccccgatacg ctgcgcctgc gcgcgccagt
    10081 aggttcggat ctcggctgag acgggcggga tcggagaaag gccctcgagc aacgccttga
    10141 agtagagtcc cgtcccgccg acgaagatcg gcaagcgccc ctgcgcctct gcctgcgcaa
    10201 tggcccgcgc cgcgtctttc atgtaccggc ccgccgaata ggcttcccgc gccggcacga
    10261 agccgtaaag cgcgtgcggg acgcgtgcct cctcctccgg cgtcggccgg gcggtgagca
    10321 cgcgcagctc tcgatagacc tgcatgctgt cggcgttgat gaccaccccg ccgtagcgtt
    10381 ccgcaagggc gagcgccaac gccgacttgc cgctggccgt cggcccggca ataaggatgg
    10441 cgcggcagga attgagagaa ttcatccgct tcgtctcagt atccgggctc tgcccgcccg
    10501 gcagcaggac aaaccataca atgcccgatc gctatgcgct cgtcctcacc agctctccgg
    10561 cagcacctgc cctcggccgg gatgccgtcg accgggcggt ttcggctttc cccgacggcc
    10621 tcagcccgga tcccggctgg ctggccgaag gcgaggcgtg gcaggcggct tttgtggcca
    10681 aaggccccgg tgagccggag cgggtccgct cggcggtcgc tgccgctctc accgatttcc
    10741 cgatagacgt caacgtcgtc gcgaatgatg ccgcacggcg caagaagctg ctcgttgccg
    10801 acatggagtc gacgatcatc gagcaggagt gccttgacga gctcgccgat catatagggc
    10861 ttcgggaccg catatcggag atcacggcac gcgccatgcg cggcgagatc gcgttcgagg
    10921 aggctcttaa agagcgcgtc ggtctgctaa agggcctcga tgccgacgtc ctgcagaagc
    10981 tttacgaccg catcactctc gtgccgggcg cacgcgagct ggtcgccacg atgcggaaga
    11041 acggagccta ctgcgccctc gtctcgggcg gctttacgtt cttcacggag cggatcgcaa
    11101 agcggctcgg cttcgatact catcaggcca accggctcgc catcgtcgac ggcaagctcg
    11161 cgggcaccgt cgcgtcgccc attctgggcc gcgaggccaa gcttgcagcg cttaagcggc
    11221 tgacccagga acttggtctt gatccccagg agacgctcgc agtcggggac ggcgccaacg
    11281 acctcgacat gatccgcgcg gccgggctcg gtgttgcttt ccgcgccaag ccgatcaccg
    11341 ccgccgaggc gaaggctgcg atccgtcacg gtgacctgac cgcgctgctc tacctgcagg
    11401 gctatcgcaa ggacgagttc gtgcgctgaa ggcgccctat cccattctca acgaagttca
    11461 tcgggccgct cagacgcat
//
LOCUS       DASVDU010000149         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1247334, whole genome shotgun sequence.
ACCESSION   DASVDU010000149 DASVDU010000000
VERSION     DASVDU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247334"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="VF226_09515"
     CDS             <1..91
                     /locus_tag="VF226_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX7074265.1"
                     /translation="AAEAKAAIRHGDLTALLYLQGYRKDEFVR"
     gene            complement(64..1230)
                     /locus_tag="VF226_09520"
     CDS             complement(64..1230)
                     /locus_tag="VF226_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7074266.1"
                     /translation="MSRLQAGGLAKAVDLIYEAALRPELWRHALNTVAEVLGSDNIGI
                     FAYPNTSAHWAVWSEGNDELVDWFFREGWHLKNPRPERALKRFGMMRVVTEADMFTPW
                     ELDNLPFNQAMIHQMGYRWDAGAMLGEIDGDAIAFTTQRLGRRERFDRQDTDAIGALA
                     PHLARAIQITARLGLNRAEGMLDALESLGCAGALIDRAGRLRRLNAKAEQLLGHGIAL
                     RHGKLCAMEKSAEPAFQRLIAEGLSPPSLERGPRLPAAVLPRRQGRPLVAYAIPVVRS
                     AQDLVQAAVILIFVDPDEHRDPVDIVLQQAFGLTAAEIRIAKGIARGEDLKDIAEKHG
                     ISIGTARAQLKSVMAKSETHRQSELVSLMMRLSGPMNFVENGIGRLQRTNSSLR"
     gene            1355..1510
                     /locus_tag="VF226_09525"
     CDS             1355..1510
                     /locus_tag="VF226_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074267.1"
                     /translation="MKAISILLAAMLVATWFAAGTQPVDAAKLIGFTKDQKKCVIKGK
                     CKSGQQP"
     gene            complement(1684..2346)
                     /locus_tag="VF226_09530"
     CDS             complement(1684..2346)
                     /locus_tag="VF226_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074268.1"
                     /translation="MAVGDVVVLSGGERNYRTPEGLLRASYDAGLAGVRLLGHGAMHV
                     LSVLLALLVLFEEWGWQPLVALVGRLRRLEVWARFEHWIEGLPPYPALAVLGLPSLAI
                     VPVKLLAVYLLARGDFVAAALVLGAAKVVGTAFIGRLFVLTKPAILRIGWAAALYDTI
                     IPWRDAMFARIRATWLWRIGRVVKRRVDLLIAAARSQLGRWRADVADWLRARRKASSR
                     LR"
     gene            complement(2366..>3067)
                     /locus_tag="VF226_09535"
     CDS             complement(2366..>3067)
                     /locus_tag="VF226_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7074269.1"
                     /translation="GAGKSTISRILFRFYDISAGRVTIDGQDIRDVTQKSLRAAIGVV
                     PQDTVLFNDTIFYNIKYGRPDATDEEVFEAAKLAQIDGFIRQLPQGYATMVGERGLKL
                     SGGEKQRVAIARTILKQPPILILDEATSALDSHTEKEIQDALDRVSRDRTTLVIAHRL
                     STIVHADNILVLEKGRLVEQGTHAQLLAKDGLYASLWNRQLRAEKAREELARTLEEAE
                     RAGALKPSDKVTPVT"
BASE COUNT          582 a         1019 c          920 g          546 t
ORIGIN      
        1 cgccgccgag gcgaaggctg cgatccgtca cggtgacctg accgcgctgc tctacctgca
       61 gggctatcgc aaggacgagt tcgtgcgctg aaggcgccct atcccattct caacgaagtt
      121 catcgggccg ctcagacgca tcataaggga cacgagctcc gactggcgat gcgtttcgga
      181 tttcgccatc acggacttga gttgagcacg tgcggtcccg atgctgatgc cgtgcttctc
      241 cgcgatgtcc ttcaagtcct ctccgcgcgc aatccctttt gcaatccgaa tttcggcagc
      301 ggtcagaccg aaggcttgct gcaggacgat atcgaccggg tctcggtgct cgtccggatc
      361 gacgaagatg agaatgaccg cggcctgaac cagatcctgc gctgagcgaa cgactggtat
      421 cgcgtaagcg acgagcggcc gcccctgccg cctcggcagc accgcggccg gcaaacgcgg
      481 tccccgctct agggaaggcg ggctgagccc ttccgcaatc aggcgctgaa aggcaggctc
      541 ggccgacttc tccatggcgc agagcttgcc atgcctgagt gcaattccgt gacccaagag
      601 ctgctcggct ttggcattca atcgacgtaa gcggccggcg cgatcgatga gcgcaccggc
      661 acaacccagc gattcgaggg catcgagcat accctctgct ctgttcaagc cgagacgcgc
      721 cgtgatctga attgcccgcg ccagatgagg cgccagcgcc ccgattgcat ctgtgtcctg
      781 ccggtcgaat cgctctcgtc tgcctaatcg ttgcgtcgtg aacgcaattg catcgccatc
      841 gatttcgccg agcatcgccc cggcatccca acgatagccc atctgatgaa tcatggcctg
      901 gttgaacggc agattgtcga gctcccaagg tgtgaacatg tccgcctcgg tgacgacgcg
      961 catcatcccg aaccgcttca aggcccgttc aggcctcggg ttcttcagat gccagccctc
     1021 acggaagaac caatccacca gctcgtcgtt accctcggac caaacggccc agtgcgctga
     1081 cgtgtttgga taggcgaaga tgccgatgtt gtcggatccc aggacctctg cgacggtatt
     1141 gagggcatga cgccataatt cggggcgcag cgcggcctcg tagatgagat ccacggcttt
     1201 tgccagcccg ccggcctgca acctcgacat gcccaccccc cagaccaacc tccacagagc
     1261 atcagacgtc tgccctttta cctaatctgg gatatgcgcg gctagaggcg ccagcctaat
     1321 tcgattgcgt gcaactttga ccggggacag tctgatgaag gcaatctcga tacttcttgc
     1381 cgccatgctg gttgcgacct ggtttgccgc cggcacccag cctgtggacg cagccaagct
     1441 gatagggttc accaaggacc agaaaaaatg cgtcatcaaa ggcaaatgta agagcggaca
     1501 gcagccgtag cggctgacgt ccaccagcgg ggctcactca tcagccgaca ttgtcggccg
     1561 ccgggcgaag gtgtgattct ccacggcggc ctgactgcgc tgctctaccg acaggggtat
     1621 cgcaagaatg aattcgcaac cgtcaggcgc agtgatgaga gctcggaagc ggctcgaacc
     1681 agcctagcgc agccgcgaac tcgcctttcg ccgtgctctc agccaatcgg cgacatcggc
     1741 gcgccacctg cccaactgcg aacgtgccgc cgcaatgagc agatccacgc ggcgcttcac
     1801 gacccgccca atccgccaga gccacgtcgc ccgtatgcgg gcgaacatcg catctcgcca
     1861 ggggatgatc gtgtcgtaga gagctgcggc ccagccgatc cgcaggatcg ccggcttggt
     1921 cagcacgaac agtcgcccga tgaaggccgt tccgacgacc ttggcagcgc cgagcaccag
     1981 ggccgcagcc acgaagtcgc cgcgcgcgag aagatagact gccagcagct tcacgggcac
     2041 gatggcaagc gagggcaacc ccagcacggc cagtgccgga tatggcggca gaccttcgat
     2101 ccagtgctcg aaacgcgccc acacctcgag gcggcgcaga cggccgacca gcgccaccag
     2161 cggctgccag ccccactcct cgaaaagcac gagaagggcc aggagcacgc tgagcacgtg
     2221 catcgcgccg tggccgagca gtctcacgcc ggcgaggcca gcgtcgtagg aggcccgcag
     2281 caagccctcc ggcgtacgat agttccgctc tcctccgctc aggaccacga cgtcacctac
     2341 tgccaatgtg gcccgttcaa gaccgtcagg tcaccggagt gaccttatct gacggtttga
     2401 gggcaccggc cctttcggcc tcctcgagcg tgcgggcgag ctcctcgcgc gccttctccg
     2461 ccctgagctg acggttccag aggctggcgt aaagtccatc cttcgccagc agttgcgcgt
     2521 gggtgccctg ctcgaccagc cggcccttct ccagcacgag gatgttgtcc gcatgcacga
     2581 tggtcgacaa ccggtgcgca atgaccagcg tcgtcctgtc ccgtgaaacg cggtcgagtg
     2641 cgtcctggat ttccttttcc gtatggctgt cgagcgcgct cgtcgcctcg tcgagaatca
     2701 ggatcggagg ctgcttgagg atcgtccggg cgatcgcgac ccgctgcttc tcgccgcccg
     2761 acagcttgag gccgcgctcg ccgaccatgg tggcgtagcc ctggggaagc tggcggatga
     2821 aaccgtcgat ctgggcaagc ttcgctgcct cgaagacctc ctcgtcagtc gcatcgggac
     2881 ggccatactt gatgttgtag aagatcgtgt cgttgaagag caccgtatcc tgcggcacca
     2941 cgccgatggc ggcgcgcagg ctcttttgcg tcacgtcgcg gatgtcctgg ccatcgatcg
     3001 tcacccgtcc ggccgagatg tcatagaacc ggaacaggat gcggctgatg gtggacttgc
     3061 ccgctcc
//
LOCUS       DASVDU010000150        14046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_996030, whole genome shotgun sequence.
ACCESSION   DASVDU010000150 DASVDU010000000
VERSION     DASVDU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996030"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF226_09540"
     CDS             complement(<1..462)
                     /locus_tag="VF226_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7074270.1"
                     /translation="MMTASLDHAVACVRRFTRFYTRRMGVLEEALLGSAFTLPEARIV
                     YEIAVRDRTTASDLVADLGLDPGYVSRLLKSLEEREFITRRPSETDARQYLLDLTPLG
                     RREFNQINRKSDQEVQKLLAPLGACDRARLVAALSTAEALLAEDRPEPQCTF"
     gene            complement(552..1859)
                     /locus_tag="VF226_09545"
     CDS             complement(552..1859)
                     /locus_tag="VF226_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEX7074271.1"
                     /translation="MDETDARAPEQPKSGQNDPKKDGLARSLGLGIITGAADDDVSAV
                     GTYASAGAQFGPAFLWLAPLLFPMMFVVTYLSSKLGQVGGKGLFDALRDHYPRWILWP
                     TLIGVMIGNTVEAAADLGGMAAALNLFIPVPIPIIVVVVATIILILQILGSYKVIRNT
                     FRWLALVLFAYIASAILAEPDPWEVLRGTFIPRIEFSREFLSMLVAIIGASLSAYIYT
                     WQSNIEVEEEIAHGRKTLAQRRGATPQELRQSRNDVLTGMLFSNVIMYFIMLSTAETL
                     HKAGETDIATAAQAAAALEPLAGEAATILFTTGIVAVGLLAVPIMTIGAAYDFCQAMG
                     WKASLNAKASEDKVFYVIIGGVTAAAVLLNFLGINPMYFLVYAGIVQGFAAPPLFLLI
                     VLMTNNRDIMGDNTNSFWLNVLAIVTLLAIFAASLGLVLTWLL"
     gene            complement(2135..2872)
                     /locus_tag="VF226_09550"
     CDS             complement(2135..2872)
                     /locus_tag="VF226_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7074272.1"
                     /translation="MDGTTVFIGLVFLLAGFIKGVIGLGLPTISMGLLAIVMPPVEAA
                     ALLIIPSFLTNVWQMLAGPSLSAVVRRLWPMMVAVCIGTWAGAGLMTGATARYGTVVL
                     GIALALYALTGLTAFRFTIAKKSEPIYGPLSGGITGLITAATGVFVIPAVPYLQAIGL
                     DKEQLVQALGLSFTVSTVALAFNVTKAGAFNLSIAGATIGALATASIGMWLGTALRQK
                     LQPAAFRRWFFIGLLLLGIYLTVRSLV"
     gene            complement(2880..3761)
                     /locus_tag="VF226_09555"
     CDS             complement(2880..3761)
                     /locus_tag="VF226_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7074273.1"
                     /translation="MRSLLFVPGDSDRKIAKALSSGADALILDLEDSVALDRKAEARA
                     MTRDFIASTKGQGKRPRLYVRINALTTDLWSADLEGVMSAGPDGIILPKPRSGEDVHR
                     LSLALDHAEERAGLKLGSTRIVAIATEVPICILNMSSFVESSSRLEGLTWGAEDLSAE
                     VGSTATREPNGVLTSPYRLARDLCLFTAVSAGVQPIDTVFIDFRDLDGLKAEAAAAAR
                     DGFTGKLAIHPDQVEIINAAFTPSRREIEHARDIVAMFAASPGAGVISLNGQMYDRPH
                     LTRAERVLARAGLAGSE"
     gene            complement(3765..4223)
                     /locus_tag="VF226_09560"
     CDS             complement(3765..4223)
                     /locus_tag="VF226_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7074274.1"
                     /translation="MRGLYYEEFEVGHVFEHAIGRTVTEMDNTLFSCLTHNPQPLHID
                     HAFAAQTEWGKPLVNSLFTLGLMIGISVSDTTLGTTIANLGMTDVNFPKPVFHGDSIR
                     VKTEVLSKRESRSRPDAGIVEFHHRAYNQRGELVAECRRMAFMRKKPKDN"
     gene            complement(4266..5048)
                     /locus_tag="VF226_09565"
     CDS             complement(4266..5048)
                     /locus_tag="VF226_09565"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX7074275.1"
                     /translation="MPKPDRKFEKIAFVASDHPEAQEALARLTKQYGNVPPETADAIV
                     ALGGDGLMLQTLHKHMNDEIPIYGMNRGSVGFLMNEYREDDLLARLEGAEISCIHPLV
                     MRARDIHGDVHEDLAINEVSLFRQTHQAAKLRILIDGKVRLEELICDGILVATPAGST
                     AYNLSAYGPILPINAPLLALTPISPFRPRRWRGALLPNVAHIQIDVLEPDKRPVSAVA
                     DHSETRSVVSVTVEEERSINLFMMFDPGHSLDERILSEQFRY"
     gene            5109..5453
                     /gene="cutA"
                     /locus_tag="VF226_09570"
     CDS             5109..5453
                     /gene="cutA"
                     /locus_tag="VF226_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7074276.1"
                     /translation="MQENDKAVLIYSTFPTAEAAEQAGTHLVDQGLAACVNIWPGMTS
                     IYMWQGQRQRDSEAVMIIKTRSKVAQAAIAEAKRHHPYTNPAFLVLPVESGAPEFLRW
                     IAEQTHEPDAPC"
     gene            5552..6199
                     /locus_tag="VF226_09575"
     CDS             5552..6199
                     /locus_tag="VF226_09575"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX7074277.1"
                     /translation="MHHKLNRQLCDELRTVPVVPVLTIENADSAVPLARALASGGLRI
                     LEVTLRTPAALEGLRRIAREVPEAMVGAGTVLKPEHGQDAIAAGARFLVSPGITPSLV
                     RAASMWTVPFLPGAATPSEVMALTDLGYCAAKFFPAEPMGGVAILKSFVAPLPNMLFC
                     PTGGIGQANAPSYLALPNVVAIGGSWVAPSDAIAAGDWGTITRLAHEASRLRALR"
     gene            complement(6202..7146)
                     /locus_tag="VF226_09580"
     CDS             complement(6202..7146)
                     /locus_tag="VF226_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX7074278.1"
                     /translation="MPLSWKSGRVVSVGECMVELSRGPDQRFGLGYGGDTFNTAVYLS
                     RAGVEVAYATALGDDPYSGGILDLARREGVATDLVLTVPGRLPGLYLIETTADGERSF
                     WYWRDRAPARELFELAGADRIAEGFREAALVYFSGVTLSLYSPTGLDRFAAALQAAKD
                     AGAKVAMDSNYRPRGWGGEIDRARRTFERFWRLADIAMPTLEDEQALWGDADAAAAAN
                     RLQGLGVLEVVVKMGARGAFVASGGSGEIVPAEANVQAVDTTAAGDSFNAAYLAARKS
                     GASAREAAARGNKLAAAVIQHRGAIVPAHATAPVLAGS"
     gene            7355..8665
                     /locus_tag="VF226_09585"
     CDS             7355..8665
                     /locus_tag="VF226_09585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX7074279.1"
                     /translation="MIGAGYVGLVSAACFSEFGWTVTCVDKDHDRVESLKRGEVPIYE
                     PGLDDLLQRNIAARRLSFSQELGPAAASADLIFLAVGTPMRRGDGHADLSYIYAAVAE
                     IAPHLTGFTVITTKSTVPVGTSREIARRLRELRPDADFAVCSNPEFLREGSAIQDFTH
                     PDRVLVGCDDPRARDIMERLYKPLALRNAPIMFVRPESAELAKYAANAFLAMKISFIN
                     EMADLCEEVGANVQEVASAIGKDRRIGDKFLHPGPGYGGSCFPKDVSALIRTAREARA
                     PLSLVEQVQRVNDERKISMASRIERAAGGSVRGRTIAVLGVTFKPNTDDMREAPSLVL
                     LPMLLERGATIRAYDPQGRKHGEKLLPGVAWCESALDAATSADIVVVLTEWNEFRALD
                     LGALKSVMRGHVLVDLRNIYQPRDALAEGFHYSSIGRPQTDLQS"
     gene            complement(8675..9481)
                     /locus_tag="VF226_09590"
     CDS             complement(8675..9481)
                     /locus_tag="VF226_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX7074280.1"
                     /translation="MRFALGVAIMRLSVGLLVLLVGVGLAVLHFQPPPQPAGIDQLVL
                     SQSAENGLTTSSTSSAGAGGGVGTKPSGSGVAPWESRTVVVASRSVPDAPWRTVIYPD
                     ETPERLSGADLARVLQEELRRVGCYHGEIDGDWGPMSKRAMSAFNERVNARLPVDSPD
                     HILLRMVRGFNGRGCGQPGSVVAGDDDSRRVQPLPGRMAVGGPVETNANAPLTRYVAV
                     EEPRAPSPEQQRQPTRRSRVAERSSPAPSYRAPRQERKSWIDRVFNPAGP"
     gene            10238..11440
                     /locus_tag="VF226_09595"
     CDS             10238..11440
                     /locus_tag="VF226_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074281.1"
                     /translation="MRHALGVIGVLAAGVLLAVSAAMNWRFGYQLGKTEFDGLIYGSA
                     SAAADCLKALVPFFFFAAIRNRMWSQAAASALVWVVVTAYSLTSALGHAALNRMDTTG
                     QRAIEAQSYQDLRANLDRAKEQLSWVPQHRPAAVVQSDIDTAKNSREWQWTDGCTKVT
                     GPKGRKFCGDYNGLLAELASAEQAEKLEARIAEIQAQLGTVDSGAVMAEADPQAAVLT
                     KLAGIFDPSLTVEDVQTALTIFIALLLEVGSGFGMYIAFSQWRLYDRTAPAAPVIRPA
                     AQADPAPVASVEVVDNAPVVANDNRSVSKLVPPETDVQRFFKERVVRKANSSVTPPEL
                     YEDYLDWCGQLGKEPLAQPIFYRDCLEMGIERHKAGNRTRYMGIALRNLPESWEDKKL
                     PALAPQAA"
     gene            11866..12360
                     /locus_tag="VF226_09600"
     CDS             11866..12360
                     /locus_tag="VF226_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7074282.1"
                     /translation="MTQVTMTVNGESVSADIEPRMLLSQFLREQLRLTGTHIGCDTSQ
                     CGACVVHVDGRAVKSCTTLAVSCAGSDVLTIEGLAKDGQLHPMQEAFREHHALQCGYC
                     TPGMIMAAIDIVNRKGTELDEHTIREELEGNICRCTGYHNIVKAVAAGAAAMGTGELR
                     QAAE"
     gene            12382..>14046
                     /locus_tag="VF226_09605"
     CDS             12382..>14046
                     /locus_tag="VF226_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7074283.1"
                     /translation="MTSTGIGAPVRRREDFRFITGHGNYVDDISRPGQAYAVFVRSPH
                     AHAKINNIDTSAARQAPGVLGVFTGKDLAADKIGGLICGWMIHSKDGSPMKVGPHPAL
                     ALEKVRYVGDHVAVVIAETLNQARDAAELVAVDYEELPAVVDVATAQNPGQPQIHAEA
                     PNNTVFEWHLGDKTAVEAAFAQAAHVTKLNLINNRLIPNAVEPRAAIGEYDRGQDSYT
                     LYTTSQNPHVARLVLSAFIGIAPEHKLRVIAPDVGGGFGSKIFIYAEETVCVWAARKV
                     GRPVKWTADRTESFLADAHGRDHVTTAELAVDGSGKILGMRVKTKANLGAYLSTFSSS
                     VPTYLYGPLLSGQYDIPAIYCEVDGVYTNTAPVDAYRGAGRPEATFVVERLVEVAARE
                     LGRDPAEFRMQNFIKSFPHQTPVIMTYDIGDYPTCMKKALELADYANFGQRKAESERK
                     GLLRGIGFSAYIEACGIAPSKAVGSLGAGVGLWESAEVRVNPVGTVEVLTGSHSHGQG
                     HETTFAQLVSHRLGIPIDQISIIHGDTDKVQMGMGTYGSRSGAVGMS"
BASE COUNT         2620 a         4498 c         4508 g         2420 t
ORIGIN      
        1 gaacgtgcat tgcggctccg ggcggtcctc agcgagcagc gcttccgccg tcgagagggc
       61 ggcgacgagg cgggcgcggt cgcaggcgcc gagcggcgcg agcagcttct gcacctcctg
      121 atccgatttc cgattgatct ggttgaactc gcgccggccc agtggcgtca ggtcgagcag
      181 gtactggcgg gcgtcggtct ccgatgggcg gcgcgtgatg aattcgcgct cctcgaggct
      241 cttgaggaga cggctgacat aaccggggtc gagcccgagg tcggcaacga gatcggaggc
      301 agtcgtccga tcacggacgg cgatctcgta gacgatgcgc gcctcgggca gcgtgaacgc
      361 actgccgagc agcgcctcct ccagcacgcc catccgccgg gtgtaaaagc gcgtgaacct
      421 gcggacgcac gccacggcat ggtcgagact ggcagtcatc attcgagcaa tccgatgagt
      481 gatgatcggc tgcgtgagcg tgtcggaaga tagttgcttt tgtcaagcat ctgcgtgcgg
      541 gccgcgcgcc gtcacagcag ccaggtcagc accagtccca ggctggcggc gaagatcgca
      601 agcaacgtca cgatggccag cacgttcagc cagaacgagt tggtgttgtc gcccatgatg
      661 tcgcgattgt tggtcatgag cacaatgagc agaaacagcg gtggcgcagc gaagccctgg
      721 acaatgccgg cgtagaccag gaagtacatc ggattgatgc cgaggaagtt gaggagaacc
      781 gcggcggccg tcacgccgcc gatgatgaca tagaagacct tgtcctcgct cgccttggca
      841 ttgaggctcg ctttccagcc catcgcctgg cagaaatcgt aggctgcgcc gatggtcatg
      901 atggggacgg cgagcaggcc gacggcgacg attcccgtgg tgaacaggat ggtggcggcc
      961 tctccggcga ggggctccag cgccgccgcg gcctgcgcgg ccgtggcgat atcggtctcg
     1021 ccggccttgt gcagcgtctc ggccgtcgaa agcatgatga agtacatgat gacgttcgag
     1081 aacagcatgc ccgtcaggac gtcattacgg gactgcctga gctcctgagg cgtggcgccg
     1141 cggcgttgag ccagcgtctt gcgcccgtgc gcgatctcct cctcgacctc gatattgctc
     1201 tgccaggtgt agatgtaggc cgacagcgac gcgccgataa tggcgaccag catcgacagg
     1261 aactctcgcg agaattcgat cctcggaatg aaggtgccgc gcaggacctc ccaagggtcg
     1321 ggctcggcga gaatggccga ggcgatatag gcgaagagga cgagggcgag ccagcggaac
     1381 gtgttccgga tcactttgta ggagccgagg atctgaagga tcagaatgat ggtggcgacc
     1441 accacgacga tgatcgggat cggcaccgga atgaacaggt tcagcgccgc ggccatgccg
     1501 ccaaggtctg cagcggcctc gacggtgttg ccgatcatga cgccgatcag cgtgggccag
     1561 aggatccagc gcggatagtg atccctcagc gcatcgaaca ggcccttgcc gccgacctgc
     1621 cccagctttg aggacaggta ggtgacaacg aacatcatcg ggaacagaag cggagcgagc
     1681 caaaggaagg ccggcccgaa ctgggcgcct gcgctggcat atgttccgac ggctgagaca
     1741 tcgtcgtcgg cggcgcccgt gatgatgccg aggcccaggg atcgggctag cccgtctttc
     1801 ttggggtcgt tctggccaga tttgggctgt tccggagcgc gggcatccgt ctcgtccatg
     1861 tcgcttcgcg aaggagcgtt tatcctaggg cttttccgat tgagctggaa acgccaacag
     1921 cggcactctg ttgacctctc ccaaaggaag aggtcggccc gaagggccgg gtaaggggtt
     1981 ccaacagcta ccaccggcgg aacccctcac cccaaccctc tcccccgggg agagggagag
     2041 taggtgcgag cctcacctgg cgtgtttccg cgtcatcgga aatgctctag ctgcaatccg
     2101 aagagagaaa ttaggttccg gggcggacgc tggctcagac gagcgagcgc accgtgaggt
     2161 aaatcccgag cagcagcagg ccgatgaaaa accagcgccg gaatgcggct ggctgcagct
     2221 tctgccggag cgctgttcca agccacatcc cgatggaggc cgtggcaagc gcccctatgg
     2281 tggcgccggc gatcgagagg ttgaaggcgc cggccttcgt cacgttgaac gccagcgcga
     2341 cggtcgagac cgtgaaggac aacccgaggg cctggacaag ctgctcctta tcgaggccaa
     2401 tggcttgcaa atacgggacc gcgggaatga cgaacacacc cgtcgcggcg gtaatgagac
     2461 ccgtgatccc accggacagg gggccgtaga tcggttcgga cttcttagcg atcgtgaagc
     2521 ggaacgcggt caggccggtc agtgcataga gcgccagcgc aatgcccagg accaccgtgc
     2581 cgtaccgcgc cgtagcaccg gtcatcagcc ctgcgcctgc ccaagtgccg atgcagaccg
     2641 caaccatcat cggccacaag cgccggacca cggcgctgag tgaaggtccg gccagcatct
     2701 gccagacgtt ggtcaggaac gatggaataa tgagcagcgc agccgcttcg accggcggca
     2761 tgacgatggc cagcaatccc atcgagatcg tgggcagccc gagcccaatc acacccttga
     2821 tgaagcccgc gagcaggaac acgagcccga tgaagaccgt ggtcccgtcc atgggaacgc
     2881 tattcgctac ccgccagccc agcccgcgcg agcacccgtt cggctcgtgt gagatgcggc
     2941 cgatcgtaca tctggccgtt gagcgagatc acgccggccc caggtgaggc ggcgaacatg
     3001 gcgacgatgt cgcgggcatg ctcgatctca cgccggctcg gggtgaaggc cgcgttgatg
     3061 atctcgacct gatcgggatg gatcgcgagc ttgcccgtga agccgtcccg cgcggcggcc
     3121 gcagcctcgg ccttgaggcc gtccaggtcg cgaaagtcga tgaagaccgt atcgatcggc
     3181 tgcacgccgg ccgagacggc cgtgaacagg cacaggtcgc gcgcgagccg atagggcgag
     3241 gtcagcacgc cattgggctc ccgcgtcgcc gtcgagccga cctccgccga gaggtcctcc
     3301 gcgccccagg tcagcccttc gaggcggctg ctcgactcga cgaacgagct catgttcagg
     3361 atgcagatcg gcacctccgt ggcgatcgcg acgatgcgcg tcgagccgag cttgagcccg
     3421 gccctctcct cggcatggtc gagcgccagg gacaggcggt gcacatcctc gcccgagcgt
     3481 ggcttcggca ggatgatgcc gtccggcccc gcggacatga cgccttcgag atcggcgctc
     3541 caaagatcgg ttgtaagcgc gttgatgcga acgtaaaggc gcggtcgctt cccctgtcct
     3601 ttggtcgagg cgatgaaatc ccgggtcatc gctcgcgctt cggccttgcg gtcgagcgcc
     3661 acggaatcct cgaggtccag gatcagggca tcggctcccg acgacagggc cttggcaatc
     3721 ttccggtcgc tgtcgcccgg tacgaagagg agtgagcgca tatctcagtt gtcctttggt
     3781 ttcttgcgca taaaggccat gcggcggcac tcggccacca gttcaccgcg ctggttgtac
     3841 gcacgatgat ggaactcgac gatgccggca tccgggcgcg accggctctc gcgcttactg
     3901 aggacctccg tcttcacgcg gatgctgtcc ccatggaaca ccggcttggg gaagttcaca
     3961 tccgtcatgc cgagattggc gatcgtagtg ccgagcgtgg tgtcggacac ggaaataccg
     4021 atcatcagcc cgagcgtgaa gaggctgttg acgagcggct tgccccactc cgtctgggcg
     4081 gcgaaggcat ggtcgatgtg cagcggctgc ggattgtgcg tgagacagga gaacagcgtg
     4141 ttgtccatct ccgtcacggt ccggccgatc gcgtgctcga agacgtggcc gacctcgaat
     4201 tcctcgtaat aaagaccccg cacggcggct tccctcacct atcgatcggt tgactccagg
     4261 tttcgtcagt agcggaattg ctctgaaagt atgcgctcgt cgagactgtg gccgggatcg
     4321 aacatcatga agaggttgat cgaccgctcc tcctcgacgg tgacgctgac cacggagcgg
     4381 gtctcggagt ggtcggcaac cgcgctgacc ggccgcttgt ccggttcgag cacgtcgatc
     4441 tggatgtgcg ccacgttggg cagcagcgca ccgcgccagc gccgcggccg aaacgggctg
     4501 atgggcgtca gcgccaagag cggcgcgttg atcggaagaa tagggccata cgccgacagg
     4561 ttgtaggccg tcgagcccgc cggcgtcgcc acgaggatgc cgtcgcagat gagctcctcc
     4621 agccgcacct tgccatcgat caggatgcgc agcttggccg cctggtgcgt ctgccggaaa
     4681 agcgacacct cgttgatcgc cagatcctcg tgaacgtcgc catggatgtc gcgtgcccgc
     4741 atgacgagcg gatggatgca gctgatctcg gcgccctcga gcctcgccag caaatcgtcc
     4801 tcgcgatact cgttcatgag gaacccgacc gaaccgcggt tcattccgta gatgggaatc
     4861 tcatcattca tatgtttgtg cagcgtctgc agcatgaggc cgtccccacc cagagccacg
     4921 atggcgtccg cggtctccgg cggtacattc ccatattgct tcgtaaggcg cgccaaagcc
     4981 tcctgggctt ccggatggtc gctcgccacg aaggcgatct tctcgaattt tcgatcaggc
     5041 tttggcatgc tgcgtgacgt ggctgaggtt tgccggacgc cgttccggct gcagggaggc
     5101 tcgctcaagt gcaggaaaac gacaaggccg ttctcatcta ctcgacgttc ccgaccgccg
     5161 aggctgctga gcaggccgga acccacctcg tagaccaggg gctggccgcc tgtgtcaaca
     5221 tctggccggg aatgacatcc atctacatgt ggcaggggca acgtcagcgc gacagcgagg
     5281 cggtgatgat catcaagacc cgctcgaagg tcgcccaggc ggccatcgcc gaagcaaagc
     5341 gtcatcatcc ttacacaaat ccggcatttc ttgtccttcc agtagagtcc ggcgcacccg
     5401 aattcctgcg gtggattgcg gagcagaccc acgagccaga cgctccgtgc tgatcagccg
     5461 gcgtcaccat agcaggacgt cacgtcctgc ttctttttta tgctggcgcc gtcgcaatcg
     5521 tgggcccagg aagaaaggag aaaacgcgat catgcaccac aagctcaatc ggcagctttg
     5581 cgatgagctt cgcaccgtcc ccgtcgtgcc ggtgctcacc atcgagaatg ctgactcggc
     5641 tgtacccttg gctcgggcac tcgcctccgg cggattgcgt attctcgagg tcaccctgcg
     5701 tacccctgcc gcgctggagg ggctgcggcg catagcaaga gaggtgcctg aggcgatggt
     5761 cggcgccggc accgtgctca agcccgagca tggccaggac gccatcgctg caggggcacg
     5821 gttcctggtc tcgccgggga ttacgccgag cctcgtccga gccgcgtcga tgtggaccgt
     5881 cccttttctc cccggggccg ccacaccctc cgaggtcatg gcgctcacgg acctcggcta
     5941 ttgtgcggcg aagttctttc cggcggagcc aatgggaggc gttgccatcc tgaagtcgtt
     6001 cgtggccccg ctgccgaaca tgcttttctg cccgacaggc ggcatcggcc aggcgaatgc
     6061 gccgagctac ctcgccctgc ccaacgtcgt ggcgatcggc gggtcatggg ttgccccctc
     6121 agatgccatt gctgctggtg attggggcac gatcacccgc ctcgcgcatg aagcctcccg
     6181 gcttcgggcg ttgcgctagg gtcatgatcc cgccagcacg ggtgctgtcg catgagcggg
     6241 cacgatggcg ccgcgatgct gaatgacggc agcagcaagc ttgttgcctc ttgccgctgc
     6301 ttcacgggct gaggctccgg atttgcgggc tgccaggtat gcagcattga acgagtcacc
     6361 tgcggccgtc gtgtctacgg cttgaacatt ggcttccgcc ggcacgatct cgcccgagcc
     6421 accgctcgcg acgaacgctc ccctcgctcc cattttcacg accacttcca agacgccgag
     6481 gccctgcagc cgatttgccg cggccgcggc atccgcatcg ccccaaagcg cctgctcgtc
     6541 ctcgagcgtc ggcatggcga tgtcggccag cctccagaag cgctcgaacg tgcgccgggc
     6601 gcgatcgatc tcgccacccc agccgcgcgg ccgatagttg ctgtccatgg cgaccttcgc
     6661 cccggcgtcc ttggccgcct gcaaggcagc ggcaaaccgg tcgagcccgg tggggctgta
     6721 aagtgaaagc gtcacgccgg agaagtagac gagagccgcc tcgcgaaaac cctccgcgat
     6781 ccgatctgct cccgccagct cgaacagctc gcgcgccggc gcccgatcgc gccaatacca
     6841 gaacgagcgc tctccgtcgg ctgtcgtctc gatgagatag agccccggca gccggccagg
     6901 caccgtcaga acgagatcgg tagccactcc ctcgcgtctg gccaaatcga gtatgccgcc
     6961 gctgtagggg tcatctccga gcgccgtggc atacgcgacc tccacgccgg ccctggaaag
     7021 ataaacggcc gtgttgaacg tatcgccgcc atagccgagc ccgaatcgct gatcgggtcc
     7081 gcgtgacagc tcgaccatgc attcgccgac cgaaaccacg cggccgcttt tccaggagag
     7141 gggcattgca gttccaacaa tctcgagtga tgcgctccag tcggcgccgc acggcgaccg
     7201 gccgatcgcg tcaggctttc ctcacgacgg ctatctcaag tgggtcacaa agaccagctt
     7261 gaacacggcg cgaaattgac atagcccgga gcagctgccg atcggtgcag cggcaaagct
     7321 ttcgaacctg cgaggcacag ctttgaacat ttgcatgatc ggtgcgggct acgtcgggct
     7381 cgtctctgca gcgtgttttt ccgagttcgg ctggacggtc acgtgtgtcg acaaggacca
     7441 cgatcgcgtt gaaagtctga aacgcgggga agttcccatc tacgagccgg gactcgacga
     7501 cctgctgcag cggaacatcg ccgcgcgccg cctctccttc tcgcaggagt tgggtcctgc
     7561 ggccgcctct gccgacctga tctttctcgc cgtcggtacc cccatgcgcc gcggcgatgg
     7621 gcatgccgac ctctcctaca tctacgccgc cgttgccgag atcgcaccgc atctcaccgg
     7681 cttcacggtc atcaccacca agtcgaccgt ccccgtggga accagccgcg agatcgcgcg
     7741 ccggctgagg gagctcaggc ccgatgcgga ctttgccgtc tgctccaatc cggagtttct
     7801 gcgcgagggc tcggccatcc aggatttcac gcatcccgac cgtgtcctgg tcggctgcga
     7861 tgatccgcgc gcccgggata tcatggaacg gctctacaag ccgctggcgc tgcgcaatgc
     7921 gccgatcatg ttcgtcaggc ccgaatccgc cgaactcgca aagtacgctg cgaacgcgtt
     7981 tctcgccatg aagatcagct tcatcaacga gatggccgat ctgtgcgagg aggtcggagc
     8041 caacgtacag gaggtggcga gcgccatcgg caaggaccgg cgcatcggtg acaagttcct
     8101 gcacccgggc cccggctatg gcgggtcctg ctttccgaag gacgtctccg ccctgatccg
     8161 caccgcgcgc gaggcccgcg cgccgctctc cctcgtcgag caggtgcaac gagtgaacga
     8221 cgagcgcaag atctcgatgg cgagccggat cgagcgtgcc gccggcggct cggtgcgcgg
     8281 caggaccatc gccgtgctcg gcgtgacgtt caagccgaat acggatgaca tgcgtgaggc
     8341 gccgagcctc gtcctcctgc cgatgctgct ggagcgaggg gcgacgattc gtgcctacga
     8401 tccgcagggg cgcaagcacg gcgaaaaatt gcttccgggc gtcgcctggt gcgaaagtgc
     8461 gctggacgcg gcgacctcgg ccgatatcgt cgttgtgctg accgagtgga acgagttccg
     8521 cgcgctcgat ctcggtgcgt tgaagagcgt catgcgcgga cacgtgctcg tcgatctgcg
     8581 caacatctat caaccgcgtg acgctttggc ggaagggttc cactacagca gcatcggccg
     8641 gcctcaaacg gacctgcaaa gctgaatgcg ccagttaggg cccagccggg ttgaagacgc
     8701 ggtcgatcca gctcttccgc tcttgccgcg gcgcccggta ggatggcgcg ggcgagctgc
     8761 gctcggcaac acgcgaccgg cgcgttggtt gccgctgttg ttccggcgat ggtgcgcgcg
     8821 gctcttccac ggcgacgtag cgcgtaaggg gagcgttggc attggtctcc actgggccgc
     8881 cgacagccat acggccgggc aacggctgga cccggcggct gtcatcatcc ccagccacga
     8941 cggatccggg ctggccacat ccccgtccat tgaaacctcg gaccatgcgc agcagaatgt
     9001 ggtccgggct gtcgactggg agccttgcgt tcacgcgctc gttgaacgcg ctcatcgccc
     9061 gcttcgacat cgggccccag tcgccgtcga tttcgccatg gtagcaacca acgcgccgca
     9121 gctcctcctg cagcacgcgt gccagatccg caccactcag tcgctcggga gtttcgtccg
     9181 gataaatcac cgtccgccag ggcgcgtcgg gaacggacct gcttgcgacg acgaccgtgc
     9241 ggctctccca cggcgccacg ccagaacccg acggcttggt tccgacgcca ccccctgctc
     9301 cggccgaaga tgtcgacgag gtggtcaatc cattttcggc cgactgggac aggacgagct
     9361 ggtcgatgcc ggcaggttgg ggcggcggct ggaaatgcag gacggcaagc cccacgccga
     9421 ccagcaaaac cagcaatccc accgacaggc gcatgatcgc cacccccaac gcgaaccgca
     9481 atcccagccg gactcgttta ggagcccagt caaaatctcc agccattccg ccacacgatg
     9541 ttgctgtctt gcgccgtgat gacacggaaa ccagggcggg aacgcttcag ttcccctggc
     9601 atgttggccg gtttaccgac caggcaggct atcgctcgct acccagatcg ccagtacact
     9661 tagcatcaaa gagcgctttc gtctgctgcc atatctggtg gagccacagt ccgcttcaga
     9721 ccacagcttg taaaaaaacg tggaaactgc aaagtccgaa tgtggataaa tgcgcgggtc
     9781 gctggggaaa atgaattcgg caaaatgccg gcggggcgtt caaatcagac ggacgtttgg
     9841 cggcgtaaaa cagcaagctc tctgaaaaaa ttaacaaaac ccttcgaatc gcatagcttt
     9901 ccgcgcgagg gggattttgt ccgtactgcg tcgcgcgtga agcttatgcc gccccgggat
     9961 atgcacaggc ggaattcagc ggcgatcacc gactgttgaa aactccggcg gcacagcgct
    10021 ccagccaccg ttcgatgcgt tcgatccagg tcatgccgag gctccctgct caggagccat
    10081 gctcgacggc aagagagccg gatcgtcttc gcgacagccg ggagcggctt ccggctttga
    10141 atcccatggc tggtccgcaa gcaactcctc gcagcagtgg agtcacgttt ttcggtgcgc
    10201 gtacggggct ccagacgatc ttggagaggg caatgcaatg agacatgctc tgggcgtgat
    10261 tggtgtctta gcggccggcg tattactcgc cgtgtcggcc gcgatgaact ggagattcgg
    10321 ctaccaactc ggcaaaaccg agttcgatgg cctgatctac ggatcggcga gcgcggcggc
    10381 ggactgcctg aaggcgcttg tgccgttctt cttcttcgcc gcgatacgaa acaggatgtg
    10441 gtcgcaggcg gccgcctccg cgctggtgtg ggtggtcgtc accgcctact cgctgacctc
    10501 tgctcttggc cacgccgcgc tgaaccggat ggatacgacc ggccagcgcg cgatcgaggc
    10561 gcagtcctac caggacctgc gcgccaacct cgatcgggcg aaggagcagc tgagctgggt
    10621 gccgcagcat cgtccagcgg ccgtggttca gagcgacatc gacaccgcga agaacagtcg
    10681 cgaatggcag tggacggatg gctgcaccaa ggtgacgggg ccgaagggcc gcaagttctg
    10741 cggcgactac aatggcttgc tcgccgagct cgcgtccgcc gagcaggccg agaagctcga
    10801 ggcccgcata gccgaaatcc aggctcagct cggaacggtc gactcgggcg ctgtgatggc
    10861 cgaggccgat ccgcaggctg ccgtcctgac gaagcttgct ggcattttcg atccgtctct
    10921 taccgtggag gatgttcaga ccgcactgac gatcttcatc gcgctgctgc tcgaggtcgg
    10981 ctcgggcttc ggcatgtaca tcgcgttcag ccagtggcgt ctctacgacc gcacggctcc
    11041 ggccgcgcct gtcatcagac cagcggcgca ggctgatccg gccccggtcg cgagcgtgga
    11101 agtggtcgac aatgcacctg ttgttgcaaa cgacaacagg agcgtctcga agctcgtgcc
    11161 accggagaca gatgtccaac gcttcttcaa ggagagagtt gtaagaaagg cgaactcgtc
    11221 cgtcacacct ccagagctgt atgaagacta tctcgattgg tgtggacagc ttggcaagga
    11281 gccattggca caacccatct tctaccgaga ctgcctcgag atgggcatcg aaaggcacaa
    11341 ggcaggaaac cgaacgcgat acatgggcat agcgctcaga aatctgccgg agagttggga
    11401 ggataagaaa ctgccggcct tagcaccaca agcagcctga ggtttggcgc cacagcccag
    11461 agcgccaaag cggagcggag catcgcgaaa gcggtgcccg ctcccagctc ccgggcaaag
    11521 cgtcaaacct ggcaaccaaa gggccgcgtt gcggccctcg atcttttcga ggcgggacat
    11581 gcgtcccgcc tcgtttttcg ttctcgctgg agagcgtggg ctatctctgg ctcggcatcc
    11641 tcggcagccc gcccggaccc ctgctcgatc ccgggccgtg gcgcgccgcc agccccaggg
    11701 cccgcgcggc gtctcgatga gaggcccacc ttcctgcggg tgcaattcca cggagagacc
    11761 cgacagcttg ctttgaagcg aaaggttttg cgaatagagt aggcaggtgc ctggacgagc
    11821 tttcaatctc gccgttccaa tggcgagacc tgcgggagga accaaatgac ccaagtcacg
    11881 atgaccgtga acggtgagag tgtgtcggcc gacatcgagc cgcgcatgct tctgtcccag
    11941 ttcctacgag agcaattgcg tctgacgggc acgcatatcg gctgcgacac gagccagtgc
    12001 ggggcgtgcg tcgtgcatgt cgacggccgc gcggtgaagt cctgcacgac gctcgcggtc
    12061 tcctgcgccg gctctgacgt cctgacgatc gaagggcttg cgaaggacgg gcagctgcac
    12121 ccgatgcagg aagccttccg cgaacatcac gcgctgcaat gcggctattg cacgccgggc
    12181 atgatcatgg ccgccatcga catcgtgaac cgcaagggga ctgagctcga cgagcacacc
    12241 attcgcgagg agctcgaggg caacatctgt cgctgtaccg gctatcacaa catcgtcaaa
    12301 gccgtcgcgg ccggcgccgc agccatgggc acgggcgagc tccggcaggc ggcagaatag
    12361 gcgacgggag gagatgacac catgaccagc acgggcatcg gcgctccggt acggcgccgc
    12421 gaggacttcc gcttcatcac gggccacggg aactacgtgg acgatatcag ccggccgggt
    12481 caggcctacg cggtgttcgt ccgctcaccg cacgcgcatg cgaagatcaa caacatcgat
    12541 acatcagccg cccgccaggc tcccggcgtg cttggcgtct tcaccggcaa ggacctcgcc
    12601 gccgacaaga tcggcggatt gatctgcggc tggatgatcc attcgaaaga cggctcgccg
    12661 atgaaggtgg ggccgcatcc ggcgctcgcc ttggagaagg tccgctacgt cggcgaccac
    12721 gtggccgtcg tgatcgctga aacgctcaac caggcgcgcg atgccgccga gctcgtcgcc
    12781 gtcgactacg aagagctccc ggccgttgtc gacgtggcca cggcacagaa tccgggtcag
    12841 cctcaaatcc acgccgaggc acccaacaac acagttttcg agtggcacct cggcgacaag
    12901 acggccgtcg aggccgcctt tgcccaggcc gcgcacgtga cgaagctcaa tctcatcaac
    12961 aaccgcctca ttcccaacgc ggtcgagccg cgagccgcaa tcggagaata cgaccgcggg
    13021 caggattcct acacgctcta tacgaccagc cagaacccgc atgtcgcgcg gctcgtgctg
    13081 tccgccttca tcggcattgc gcccgagcac aagctgcggg tgattgcgcc ggatgtcggc
    13141 ggtggcttcg ggtcgaagat cttcatctat gccgaggaga cggtctgcgt ctgggcggcg
    13201 cgcaaggttg ggcgccccgt gaagtggacg gccgatcgta cggaaagctt ccttgcggac
    13261 gcccacggcc gcgatcacgt cacgacggcg gagctggccg tggatggctc gggcaagatc
    13321 ttgggcatgc gcgtcaagac gaaagccaac ctcggcgcct acctttcgac gttctcctcc
    13381 tcggtcccga cctacctcta cggccctctg ctctccggac agtacgacat tccagcgatc
    13441 tactgcgagg tggatggagt ttacaccaac accgctccgg tggatgccta ccgtggcgcc
    13501 ggccggccgg aggccacgtt cgtcgtcgag cggctggtgg aggttgcggc gcgggagctc
    13561 ggccgcgatc cggcggaatt ccggatgcag aacttcatca agagcttccc gcatcaaacg
    13621 ccggtcatca tgacctatga catcggcgac tacccgacct gcatgaagaa ggcgctggag
    13681 ctcgccgact acgccaactt cggccagcgc aaggcggaaa gcgagcgcaa gggcctgctg
    13741 cgtggcatcg gcttctcggc ctacatcgag gcttgcggca tcgcgccgag caaggcggtg
    13801 ggctcgctcg gcgccggcgt cggcttgtgg gaatcggccg aggtccgggt caatccggtc
    13861 ggaacggtcg aggtgctcac cggctcgcac agccacggcc agggtcacga gacgaccttc
    13921 gcccagctcg tctcgcaccg gctcggcatc ccgatcgatc agatctcgat catccacggc
    13981 gataccgaca aggtgcagat gggcatgggc acgtacggct cacgctcagg cgccgtcggc
    14041 atgtcg
//
LOCUS       DASVDU010000151         3539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1421955, whole genome shotgun sequence.
ACCESSION   DASVDU010000151 DASVDU010000000
VERSION     DASVDU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421955"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1179)
                     /locus_tag="VF226_09610"
     CDS             complement(175..1179)
                     /locus_tag="VF226_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074284.1"
                     /translation="MSGKASIIIGFTPFHLLPMIEVLQAMEGDAYFFHPSADDPCIGT
                     TEQCLAFLGKCNDPHLHRIEKYFRAGRDLDRIAKRYGEVDVYVPHPFNPLSNRAFFHP
                     SASGRFIYQDGLLNYYDALSPLSSRGRRLRQMAKAAAVGLLYRTYEGHLSGVDSRTVS
                     GGFFTHPDRIVRAGSFPVLRRLDFGRGDKGGRPIPDGGTLFLDQPIEEVVDAGVAREL
                     RRQTLDYVNRLGGRVYYKPHYSQKDARCFDAAWRPVGRELGALPAEWVAPKLDVANVV
                     SFFTSALANVAMSNDAVTCHATAAHAVPISIDGRRTSLAELLSGFGVKTVDLLPPRHR
                     "
     gene            complement(1241..2284)
                     /gene="pseI"
                     /locus_tag="VF226_09615"
     CDS             complement(1241..2284)
                     /gene="pseI"
                     /locus_tag="VF226_09615"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HEX7074285.1"
                     /translation="MISIAGRQIGSDKPPYVIAEMSANHNGRLDTALKIVAAAKAAGA
                     DAVKVQTYRPDTITLNSDSEEFRIRGGLWDGRTLHELYEEAHTPWEWHPSLFKHARKL
                     GITIFSSPFDRTAVDMLEDLGAPAYKIASFEAVDLPLIKYVASTGKPMIISTGMADAE
                     EIQEAIDAAHAGGCRELAILRCVSGYPAPASDYNLRTIPDMIERFGVVTGLSDHTLDN
                     ATAIASVALGASIVEKHFTLDRSGGGPDDSFSLEPLELAALCTGVRTAWAALGSVDYG
                     RKSSEQGNVKFRRSLYFVKDMQAGDLITADAVRSVRPGYGLPPKFLDSVIGRKLARAV
                     RANTPVAWEHIET"
     gene            complement(2281..3231)
                     /gene="pseH"
                     /locus_tag="VF226_09620"
     CDS             complement(2281..3231)
                     /gene="pseH"
                     /locus_tag="VF226_09620"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR03585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HEX7074286.1"
                     /translation="MVENDLPLVLSWRNAEDVRKNMYTSHIITEQEHRSWWDAQQSNP
                     ASRLLIFEIAGEPAGVVTFSNYTGEGGTATWAFYSGDRTRRGVGSMMERSALEYAFEK
                     LKVRRLECEVLSFNRAVVNFHVKHGFTIEGVFRLAFKRCDEYFDIYRLSMLADEWTKH
                     VKPALTAMADGQASFVGKELVSELDLSTAAVSAYAAATGDKNPIHSDVDYARAMGFPG
                     RVAHGMLVGTELSRIFSSDFPGPGTIYVSQSMEFKAPLFVDVQAQIKLKVISQIGRRL
                     YVETHVHQDGRLCVTGIAVLMIPKGGVANTTSSAVLGNSE"
     gene            complement(3249..>3539)
                     /locus_tag="VF226_09625"
     CDS             complement(3249..>3539)
                     /locus_tag="VF226_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HEX7074287.1"
                     /translation="DLAIGAAGSTSWERCALGLPTLVVVLAMNQYEVAMNLEKSGAAT
                     IVSLHEPGKGIKAALLGMSGALLEAQSHNAALLVDGNGTQRVVEVLNDRGGL"
BASE COUNT          703 a         1146 c         1009 g          681 t
ORIGIN      
        1 tgatcgccgc cctgctctgc gtgcccgggt tcgggctcaa gggcgcggtg gccatgctgc
       61 tgatcacgca agccgccacc gcgctgctgc tctgggcgct cgcacgattc ggacgcgccg
      121 cgtcggactc gtaagcagcg ccccaagtca ccggtacatg cgccggcaat ccgctcaccg
      181 gtggcgcggc gggagaaggt cgaccgtctt cacgccgaag cctgacaaca gctccgccag
      241 cgacgtccgg cgtccgtcga tcgaaatcgg caccgcgtgc gcggccgtcg cgtggcacgt
      301 gacggcgtcg ttgctcatgg cgacgttcgc caacgccgac gtgaaaaagc tcaccacgtt
      361 ggccacgtcc agtttcggcg ccacccattc cgcgggcagc gcgccgagtt cgcgcccgac
      421 cgggcgccat gccgcgtcga agcacctcgc gtccttctgc gaatagtgcg gcttgtagta
      481 gacgcggccg ccgaggcggt tgacgtagtc gagcgtttgc cgccgcagtt cgcgcgcgac
      541 gccggcatcg acgacctcct cgatcggctg atccaggaac agggtgccgc catcaggtat
      601 cggccggccg cctttatcgc cgcggccgaa gtcgagccgt cgcaaaaccg gaaaactgcc
      661 cgcccggacg attctgtccg gatgcgtgaa gaagccgccg gataccgtcc gactatctac
      721 accgctgaga tgcccttcgt acgtgcgata cagaagcccc acagcggccg ccttggccat
      781 ctgcctcagg cgccgtcccc gggaagaaag cggactcaac gcatcgtaat agttgagcag
      841 cccatcctga taaatgaacc tcccactcgc gctgggatgg aagaacgcgc ggttggagag
      901 tggattgaag ggatgcggaa cataaacatc gacctcgcca taccgcttcg cgatccggtc
      961 gaggtcgcga ccggcgcgga agtacttctc gatccggtgc aggtgcggat cgttgcactt
     1021 ccccaggaac gcgaggcatt gctcggtagt gccaatgcag gggtcatctg cagacggatg
     1081 aaagaagtac gcgtcgccct ccatcgcctg cagcacctcg atcatgggca agaggtgaaa
     1141 aggcgtgaaa ccgatgatga tggaggcctt gccgctcatg gtgtgccgct cttgtccttg
     1201 ctccgcacat gagcgcgctc ggcacacgca tcgagccgcg tcatgtctcg atatgctccc
     1261 acgcgaccgg cgtgttcgcc cgcacggcgc gggccagctt cctgccgatc acgctgtcga
     1321 ggaacttggg gggaaggccg tagccaggcc gcacgctgcg gaccgcatcg gcggtaatga
     1381 ggtcgccggc ttgcatgtcc ttcacgaagt acagcgaccg cctgaacttc acgttaccct
     1441 gttcgctgga cttgcggccg tagtccacgc tgccgagcgc cgcccacgcc gtgcgcaccc
     1501 cggtgcagag agcggcaagc tcgaggggct ccagcgaaaa actgtcgtcg gggcctccgc
     1561 cgctgcgatc cagggtgaag tgcttctcta cgatggacgc gcctagcgcc acgctggcga
     1621 tggcggtcgc gttgtcgagc gtgtgatcgg acagaccggt gaccacgccg aagcgctcga
     1681 tcatgtccgg gatggtgcgc aggttgtagt cggacgcggg cgcgggatag ccgctgacgc
     1741 agcgcaaaat ggccagctct cggcagccgc cggcatgcgc cgcgtcgatc gcctcctgga
     1801 tttcctcggc gtcggccatg ccggtggaga tgatcatcgg cttgccagtg ctcgccacat
     1861 acttgatcag cggcagatcg accgcctcga aagaggcgat cttgtaagcg ggtgcgccaa
     1921 gatcctccag catatccacc gccgtgcgat cgaacggcga actaaaaata gtgatgccga
     1981 gcttgcgcgc gtgcttgaac agcgacggat gccactccca cggtgtatgc gcctcttcat
     2041 acagttcgtg gagcgtgcgg ccgtcccaca gcccgccgcg aatcctgaac tcctcgctgt
     2101 ccgaattcag cgtgatcgtg tccggccggt aggtctgcac cttgaccgcg tcggcacccg
     2161 ccgccttcgc cgccgccacg atcttcaaag cggtgtccag cctgccgttg tgattcgctg
     2221 acatttccgc gatgacgtaa ggcggtttat cagaaccaat ttgacgtccg gcgatactga
     2281 tcattccgaa ttccccagta ccgcactgga tgtggtattg gcgacgcctc cctttggaat
     2341 catcaagacg gcgatccctg tcacgcacag ccttccatct tgatgaacgt gagtctccac
     2401 ataaaggcgc cgaccaatct gcgagatgac cttcaatttg atctgcgcct gaacatccac
     2461 aaacaagggc gctttaaatt ccatactttg cgagacataa atggtgcccg gtccggggaa
     2521 atcactggaa aagatgcgtg atagctcggt acccaccagc atgccgtgcg caacgcgacc
     2581 cgggaaaccc atggctcttg cgtaatccac atccgaatgg atcggattct tgtcgcctgt
     2641 tgcagccgca taggcactta cggcagccgt ggacaagtcc aactcggata caagctcttt
     2701 ccccacaaag cttgcctgcc cgtcggccat ggcagtcaaa gccggcttta cgtgcttcgt
     2761 ccattcatcg gccagcatcg aaagtcgata tatgtcgaag tactcatcac aacgtttaaa
     2821 agccagcctg aagacgccct caatagtgaa tccatgcttg acatgaaagt tgacaacggc
     2881 gcgattgaac gaaagcacct cgcattccag cctcctgact ttcaatttct caaaggcgta
     2941 ctctagcgcg ctgcgctcca tcatgctgcc aacgccgcga cgcgttcggt cacccgaata
     3001 aaaggcccac gttgccgtgc cgccttcgcc tgtgtagttt gagaaagtaa ctacgcctgc
     3061 cggctcacct gcgatttcaa agatcaacag ccgagacgcc ggattgctct gctgcgcatc
     3121 ccaccacgag cgatgctcct gctcagtgat gatatggctt gtatacatat ttttccgcac
     3181 gtcctcggcg ttccgccatg acaggactag cggaagatca ttctcgacca tggctcttag
     3241 gctagagttc ataagccgcc cctgtcatta agcacttcca ccactctttg cgtgccgttt
     3301 ccatctacca ataatgcggc attgtgggat tgagcttcca ggagcgcacc ggacatccct
     3361 aacaacgcag cctttatgcc cttgccaggc tcatggagcg aaacaattgt ggcagcacca
     3421 cttttctcaa ggttcatggc cacctcatac tgattcatcg ccagcaccac taccaatgtc
     3481 ggaaggccga gcgcgcaacg ctcccacgac gtgctgcccg ccgcaccaat agcgagatc
//
LOCUS       DASVDU010000152         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_974755, whole genome shotgun sequence.
ACCESSION   DASVDU010000152 DASVDU010000000
VERSION     DASVDU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974755"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VF226_09630"
     CDS             <1..792
                     /locus_tag="VF226_09630"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEX7074288.1"
                     /translation="FCGTLRRYRPDGLSLVLTSYASHARRPRHVMVLKPSALPRFPTR
                     LAGNAVPRTMGLLLGGQSGECRYDTDEWKQLLDFAEKSYHTLGIRWVVSNSRRTLQAV
                     SEMVAARVAAGSRSITSFIDVRITGPGTLGHVLEQVQAVVCTDDSSTMISECVSAGLP
                     VLGVRPRRAKFTPDEQSYRAYLIENGWHRSIPIAALTPDRLLNEMACIQPLTEDPLEH
                     LATVIRERIPELFSAYPAQVTHSDQRASRRTESRSSRRTSTLSPA"
     gene            complement(726..1214)
                     /locus_tag="VF226_09635"
     CDS             complement(726..1214)
                     /locus_tag="VF226_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like family protein"
                     /protein_id="HEX7074289.1"
                     /translation="MPTNLLRARAKPPAKRDPRERALATAGTDRFVLIRAGTTSVRAR
                     LLDTPTADRIWRALPIFSVAQLWGQGEIFFETPLESGRERTAKIIVEPGAIVFSPDRD
                     VIGIPFARTPISRSGELRLWSPSNVWAEALDDVGVFRTVQAGERVEVRRLDRDSVRRE
                     AR"
     gene            complement(1328..2572)
                     /locus_tag="VF226_09640"
     CDS             complement(1328..2572)
                     /locus_tag="VF226_09640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7074290.1"
                     /translation="MTNASGGRRVVVTGMGVVTPIGQNVQEFWAGLKRGQSGVSPLEG
                     FPLEDLKILIGAQIKNFDPRERLKGAKRDKIIMHADRYSWFAAAAADEAVRQSGLEMP
                     LENPYRAACIIGSGAGGLTTYEIAYRDLFIHNKRATHPLTLLRIIGSSASAHVGIEYG
                     VKGPTFATCSACSTATHAIGIARDYIRNNVVDVAIAGASEAVINYGTMKAWQALHVLS
                     PEGCFPFAKKRNGTVLGEGAGILVLEEYEHARKRGATILAELIGYGMTSDSKDMVNPD
                     VEGPREAMRLALEDAKIGPADIDYLNAHGTATTINDKNETNAIKEVFGQHAYKLAISS
                     TKSMHGHPLGAGGGIEAVACIKAINEGWVPPTIGLDEPDPECDLDYIPNVGRDMKVRF
                     AMSNSFAFGGLNAVLVFAAPPG"
     gene            complement(2639..2923)
                     /locus_tag="VF226_09645"
     CDS             complement(2639..2923)
                     /locus_tag="VF226_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7074291.1"
                     /translation="MDEVATKIIDILKKHMKEPRDDISLSTALTDLKIESLDLAMIVF
                     DIEDTFGIEIPYNANEEAADFKTVGSVVERVKALIESAGTGGATASAKAS"
     gene            3290..5044
                     /locus_tag="VF226_09650"
     CDS             3290..5044
                     /locus_tag="VF226_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7074292.1"
                     /translation="MPAGTSNETGGSRPVAAATYPWIASYPPGVSWDQEFTARPIYTL
                     LDDAVAAHAASPFTNFLGRVTTYGEIGEAVSRAAAGLQKIGVGKGSKVGLLLPNSPTF
                     IIYYFAVLKLGATVVNYNPLYTVDELAFQIRDSETELMVTLDLAVLFEKLEKLLQSGV
                     LARAVVCSFASLLPTAKAILFRMLKGRQLAHVGRSPMAHRIVLEADLMENDGRFEPPS
                     IDVENDIAVLQYTGGTTGTPKGAMLTHANIYINVQQVMAFAPTLEVAKHRVLAILPFF
                     HVFAMTVVLNFGVAKAAEMVIMPRFVLDEALDLIEETRPTVMPGVPTLFNTMMNHPRF
                     KSLDLSSLRYCLSGGSALPLEVKQRFEELTGCKLVEGYGLSETSPVVTANPLEGAEKP
                     GSIGLPVPGTIVSLRALTDPTREVPLGERGEVCVRGPQVMKGYWRNPEETASQFIDGY
                     LRTGDVGVMDEDGYFYIVDRIKDLIICSGFNVYPRRIEEALFEHPAVEDASVIGIEDP
                     HRGEAPKAFIKLKDGMTATEAEIFAHLESKLSRIELPAAIEFRDGLPKTLIGKPSRRA
                     LKREEGERRESKEEAQGA"
     gene            5225..5599
                     /locus_tag="VF226_09655"
     CDS             5225..5599
                     /locus_tag="VF226_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074293.1"
                     /translation="MLRKASLLFIGSLATTVFASSLYVPSVAADDETGLASIHEQRRE
                     GNRICMSSHFHNGSSSGQKTRKLAEAAAIKDWAGFTAWEYGTIWAHYKLAASKSMNCS
                     EEGGTWRCQLEARPCRPLPKRR"
     gene            complement(5651..>6509)
                     /locus_tag="VF226_09660"
     CDS             complement(5651..>6509)
                     /locus_tag="VF226_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX7074294.1"
                     /translation="CLARLEKLKKISAQLIEITTAKAAHVSAKVDAALNAGTAPKRAA
                     EQPAPRAPPKVVARREPATPQPPPARPSPPVRELEQPPVPPSASPGGRWETDTVANLS
                     PGTERMPAPEALSALPPRLQQPEDLEFTADEIRDAGRGFFGSLSAELAAVIEYAFRSY
                     GRPNGYVLGSEGGAALLAGLRYGDGRLVTKRGEQKVYWQGPSLGYDVGLAGSRVMFLV
                     YNLEQPNDLFARFVGVDGSAYIVGGVGMTFLKKGSVVLAPIRTGLGLRVGANVGYLKF
                     TPEPSINPF"
BASE COUNT         1175 a         2064 c         2071 g         1199 t
ORIGIN      
        1 ttctgtggca cactgcgccg ctatcgtccc gacggcctga gcctcgtgct gacctcatac
       61 gcctcgcatg ccagacggcc gcggcacgtg atggtgctga agcccagcgc actcccacgc
      121 ttccctaccc ggctcgccgg aaatgcggtg ccccgtacga tgggcctcct gctcggcggc
      181 cagtccggcg aatgcaggta cgataccgat gaatggaagc agctactcga tttcgccgag
      241 aagtcctacc acacactcgg cattcgctgg gtggttagta actcgcgtcg gaccctacag
      301 gcggtgagcg agatggtagc agcccgggtc gcggcaggct ccagatcgat cacctctttc
      361 atcgacgtgc gcataaccgg tccgggcacg ctcggccatg tgttggagca ggtgcaggcc
      421 gtggtctgca cagatgattc gagcacgatg atctccgagt gcgtgtcggc cggattacca
      481 gttctgggcg tacggccgcg ccgggcgaaa ttcacgccag acgaacaaag ctatcgcgca
      541 tatctcatcg agaacggctg gcatcgctcg attccgatcg cagcactgac gcccgacagg
      601 ctgctcaatg agatggcctg catccagccg ctcactgagg atccactcga gcacttggca
      661 accgtcatac gcgagaggat tccggagctg ttttcagcct atcctgccca ggtaacccac
      721 tccgatcagc gcgcctcgcg acgtacagag tcgcgatcga gccggcggac ctccaccctc
      781 tcgcccgcct gaaccgtccg aaacacgccg acatcgtcca gcgcctcggc ccagacgttg
      841 gagggcgacc aaaggcggag ctcgcccgac ctggagatcg gcgtgcgggc gaacgggatg
      901 ccgatgacgt cgcggtcagg tgagaagacg atggcgcccg gctccacgat gatcttcgca
      961 gtgcgttccc gcccgctctc cagcggcgtt tcaaagaata tttcgccctg cccccagagc
     1021 tgcgcgacgg agaagatcgg caaggcccgc cagatgcgat ccgccgtcgg cgtgtccagc
     1081 agacgggcac gaacggacgt ggtgccggcc cggatcagca cgaaccggtc ggtgccggcg
     1141 gttgccaacg cgcgctcgcg cggatcgcgc ttggcaggcg gcttggcacg ggcgcgcaga
     1201 agattcgtgg gcatggggca aatgagcctc acgaaccctc gttgcgcaag aggcagcttg
     1261 tgacggacgc gcggtgaggc atacgggtcc gctcccggca agcggcgccg ggtcggcggg
     1321 cgggacgtca gcccggcggc gccgcgaaca cgagcaccgc gttgaggccg ccgaacgcga
     1381 acgagttcga catcgcaaac cgcaccttca tgtcgcgccc gacgttcggg atgtagtcga
     1441 ggtcacattc gggatcgggc tcgtcgagac cgatggtggg cggtacccaa ccttcgttga
     1501 tcgccttgat gcaggcgacc gcctcgatac ccccaccggc gccgagcgga tggccgtgca
     1561 tcgatttcgt cgacgagatg gcgagcttgt aggcgtgctg gccgaacacc tccttgatgg
     1621 cgttcgtctc gttcttgtcg ttgatggtcg tcgcggtgcc gtgcgcgttg aggtagtcga
     1681 tgtcggccgg gccgatcttc gcatcctcga gcgcgagcct catcgcctca cgcggcccct
     1741 cgacgtcggg gttcaccatg tccttgctgt cggaggtcat cccatagccg atgagctcgg
     1801 ccaggatggt ggcgccacgc ttgcgcgcgt gctcgtactc ttcgaggacg aggatgccgg
     1861 caccctcccc cagcaccgtg ccgttgcgct tcttggcgaa cgggaagcag ccttccggcg
     1921 agaggacgtg cagcgcctgc caggctttca tcgtcccgta gttgatgacc gcttccgacg
     1981 cgccagcgat cgcgacgtcg accacgttgt tgcggatgta gtcccgcgcg atgccgatgg
     2041 catgcgtcgc cgtcgagcag gcactgcagg tcgcgaacgt cgggcccttc acgccgtact
     2101 cgatgccgac gtgtgccgag gccgaggagc cgatgatgcg cagcagcgtg agcggatgcg
     2161 tcgcgcgctt gttgtggatg aaaaggtcgc ggtaggcgat ctcgtaggtc gtcaggccgc
     2221 ccgcgcccga gccgatgatg caggccgcgc gataagggtt ctcgagcggc atctcgagac
     2281 ccgactgacg gaccgcctcg tcggcggcag cggccgcgaa ccacgagtag cggtccgcat
     2341 gcataatgat cttgtcgcgc ttggctccct tgagccgctc tcggggatcg aagttcttga
     2401 tctgcgcccc gatcaggatc ttgagatcct caagcggaaa gccttcgagc gggctaacgc
     2461 ccgactggcc acgcttcaac cccgcccaga attcctggac gttttggccg atcggcgtca
     2521 cgacacccat gccggtgacg acgacacggc gcccgccgct cgcgtttgtc ataactgaac
     2581 gtcctgctgg gcttctatgc cccgattagt tgtaagtgcg ccttgttggc ggcttcttct
     2641 aggaagcttt ggcgctcgcc gtggccccgc cagtccctgc gctctcgatg agtgccttga
     2701 cacgctcgac gacggacccg accgtcttga agtcggccgc ttcctcgttc gcgttgtagg
     2761 ggatctcgat tccgaaggtg tcctcgatgt cgaagacgat catggcaagg tcgagcgact
     2821 cgatcttgag gtctgtgagc gctgtcgaca agctgatgtc gtcgcgcggt tccttcatgt
     2881 gcttcttcag gatgtcgatg atcttcgtcg ccacttcgtc catttcgctc tcccaccctg
     2941 cttttgcagg ttgatccggg tttatctggc ttggacactc agtagtccag gcaggtctgg
     3001 cgaacaagct cgcgcgtcac ataaccgtaa atggttgatg tgtccagccg agctgcctcg
     3061 acctgcgttg ctcgggtaag ctgatgccgc ccagcaatcg cgtcttatct tgtcagttct
     3121 taataaagtt cacagtcacc tacggcacag gcatgcagga ctggcgtgat gcggcggaat
     3181 tatgttcaat ggacggccag atctctgcgg ccaagtgccc gacaagcata atcggggagt
     3241 ctctaccggg ggcaagcagt tttattccat cggacaggga gggcaagcca tgcctgcggg
     3301 tacttcgaac gagaccggcg gctcacgacc agtcgccgcg gcgacctatc cgtggatcgc
     3361 cagctatccc cctggcgtga gctgggacca ggaattcacc gcccgcccca tctataccct
     3421 cctcgacgat gccgtggccg cacatgcggc aagtccattt acgaacttcc tggggcgggt
     3481 tacgacctat ggtgagatcg gcgaggccgt ctcccgcgcg gcagccgggc tgcagaagat
     3541 cggtgtcggc aagggctcga aggtcggcct tctgctgccc aacagcccga cgttcatcat
     3601 ctactacttc gccgtcctga agctcggcgc gaccgtcgtc aactacaatc cgctctacac
     3661 cgtggacgag ctggccttcc agatcaggga cagcgagacc gagctgatgg tcacgctcga
     3721 tctcgccgtc ctgttcgaga agctggaaaa attgctgcag tcgggcgttc tcgcgcgcgc
     3781 cgtcgtgtgc tcgttcgcca gcctcctgcc gaccgccaag gcgatcctgt tccgcatgct
     3841 caagggccgg cagctcgccc atgtcggacg atcgccgatg gcccaccgca tcgtcctcga
     3901 ggccgacctg atggagaacg acggccgctt cgagccgccg tccatcgacg tggagaacga
     3961 catcgcggtg ctccagtaca ccggcggcac gaccggcacg cccaaggggg ccatgctgac
     4021 gcacgccaac atctatataa atgtgcaaca ggtgatggcc ttcgcgccga ccttggaagt
     4081 cgccaagcac agggtgctgg ccatcctgcc cttcttccac gtcttcgcaa tgacggtggt
     4141 cctgaatttc ggtgtcgcca aggcagcgga gatggtgatc atgccgcgct tcgtgctcga
     4201 cgaggcgctg gatctcatcg aagagacgcg cccgacggtg atgccggggg tgccgacgct
     4261 gttcaacacg atgatgaacc atccgaggtt caagagcctc gacctgtcct ccctgcgcta
     4321 ctgcctctcg ggcggctccg cgcttccctt ggaggtcaag cagcgcttcg aggagctgac
     4381 gggctgcaag ctggtcgagg gttacggcct ttcggaaaca tcacccgtgg tgacggccaa
     4441 tccgctcgag ggggcggaga agcctggctc catcggcttg cccgttccgg ggacgatcgt
     4501 gtcgctgcgg gctctcaccg acccgacccg ggaggtgcct cttggcgagc ggggtgaggt
     4561 ctgcgttcgc ggtccacagg tgatgaaggg ctactggcgc aatccagaag agaccgccag
     4621 ccagttcatc gacggttatc tccggaccgg agacgtcggc gtgatggacg aggacggcta
     4681 cttctatatc gtcgatcgca tcaaggacct gatcatctgc tccggtttca atgtctatcc
     4741 ccggcggatc gaggaagcgc tcttcgagca cccggctgtg gaggatgcga gcgtcatcgg
     4801 catcgaggat ccgcaccgcg gcgaagcccc gaaggctttc atcaagctga aggacgggat
     4861 gacggcaacc gaggcggaga tattcgctca cctcgaaagc aagctgtcgc gcatcgagct
     4921 acctgcagcg atcgagtttc gcgacggatt gcccaagacg ttgatcggca agccgtccag
     4981 aagggcgctg aagcgcgagg agggcgagcg ccgcgaatcg aaggaagagg cgcaaggggc
     5041 ctgattgcgc catttccatg gagtgggcgt cataattggg agtacacgcg ttacccgacg
     5101 ccaacattgt ctcaatcctg caacacttcg gcgccgttga ttggcaacgg ctatcgtcgc
     5161 tgtatgaaaa tccttaatcc gcctgctagc aaaatgcccc gtgtgattct tgtcgggggg
     5221 cacaatgctc aggaaggcat cgctattatt tattggaagt ctagcaacaa ctgtcttcgc
     5281 gtcgtctttg tatgttccgt cggtcgccgc ggatgatgaa accggactag ccagcattca
     5341 cgagcagcgc cgcgagggta accggatctg catgtcgagc cattttcaca acggctcgag
     5401 ctccggtcag aagacccgca agctggcgga ggcggctgcg atcaaagact gggccggctt
     5461 cacagcgtgg gagtacggca ccatctgggc ccactataag cttgctgcca gcaagtccat
     5521 gaactgttcg gaagaaggcg gaacctggcg ctgccagctg gaggcccgcc cctgccgacc
     5581 actgccgaag cggcgctgac gcccgcgccc agcaccgccg ttgaaacgcg cgccagaaaa
     5641 ggcagcacac tcagaacggg ttgatgctgg gctcaggcgt aaatttcagg taaccgacat
     5701 tggcaccgac ccggagccca agaccggtcc ggatcggcgc caatacgact gagcccttct
     5761 tcaggaacgt catgccgacg ccgccgacga tgtaggcgga tccatcgacg ccgacgaagc
     5821 gcgcgaaaag atcgttgggc tgctcgaggt tgtagacgag gaacatgacg cgcgagccgg
     5881 ccagccccac gtcatagccg agcgacggtc cctgccaata gaccttctgc tcaccgcgct
     5941 tcgtcacgag gcggccgtcg ccgtagcgaa ggccggccag cagtgcagca ccgccttccg
     6001 agccgagaac atagccattg ggccggccat agctccggaa ggcatattcg atgaccgccg
     6061 ccagctccgc cgaaagcgag ccgaagaatc cgcggcccgc gtcgcggatc tcatctgcag
     6121 tgaactccag atcctcgggt tgctgtagcc ggggcggcaa ggctgaaagc gcttcaggcg
     6181 ctggcatgcg ttcagtgcca ggcgaaaggt tggccacggt gtcggtctcc cagcggccac
     6241 cgggcgaggc tgagggagga acgggaggct gttcgagctc ccgcaccggg gggctgggcc
     6301 gggccggcgg cggttgcggg gtcgcgggct cgcggcgagc caccactttg ggcggcgccc
     6361 tcggcgctgg ctgctccgcg gcgcgcttgg gtgccgtccc ggcgttcagg gcggcatcga
     6421 ccttggctga aacgtgagcc gccttggccg tcgtgatctc gatgagctgg gccgaaatct
     6481 tcttcagctt ctccaatcgg gcgagacag
//
LOCUS       DASVDU010000153        47591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_67490, whole genome shotgun sequence.
ACCESSION   DASVDU010000153 DASVDU010000000
VERSION     DASVDU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 47591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47591
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67490"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..2625
                     /gene="uvrB"
                     /locus_tag="VF226_09665"
     CDS             7..2625
                     /gene="uvrB"
                     /locus_tag="VF226_09665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX7074295.1"
                     /translation="MTAVLEALLARPEQRTDKGWALLAQEPMIASHPLVSGSMPMFMP
                     HRPPRPEKSEGGIPFRLVSEFEPQGDQPQAIAELVEGVNQHERDQVLLGVTGSGKTFT
                     MANVIARTQRPALILAPNKTLAAQLYGEFKSFFPENAVEYFVSYYDYYQPEAYVPRTD
                     TYIEKESSINEQIDRMRHAATRALLERDDVIIVASVSCIYGIGSVETYTAMTFTIRVG
                     ERLDQRQLLADLVALHYRRSDQNFIRGTFRVRGDTVELFPAHLEDRAWRISLFGDEIE
                     SITEFDPLTGQKTDELQLVKVYANSHYVTPKPTMQQALKAIKAELKQRLDELYSAGKL
                     LEAQRLEQRTLFDMEMMEATGSCAGIENYSRYLTGRKPGEPPPTLFEYLPDNALVFVD
                     ESHVTVPQIGGMFRGDYRRKATLAEYGFRLPSCMDNRPLRFEEWDAMRPQTIYVSATP
                     GSWELEQTGGAFVEQVIRPTGLTDPEVIVRPARNQVDDLLDEVRNVTRAGYRTLVTTL
                     TKRMAEDLTEYMHEQGIRVRYMHSDIETLERIEIIRDLRLGAFDVLIGINLLREGLDI
                     PECGLVAILDADKEGFLRSETSLIQTIGRAARNVDGRVILYADQMTGSLERAIAETNR
                     RREKQKAWNEAHGITPESVRKNIADVMASIYERDHVTVDAGLAEAGQVLVGHNLQAVI
                     ADLEKRMREAAADLEFETAARLRDEIKRLRETELAIADDPLARQADIEERAGRFEGNR
                     KYGGAPSARESARNEGKPGSRISREYEPVQPTYAQSTSRPHKPSLDDMGPGTDRPLPA
                     RSPRVDPRTRAGAFGEAIRGPHKPTLDEMGPHAERGLPAGKTAPPKPIKTLELPDETE
                     RKRRRGRPRKTGRPGQ"
     gene            2675..3100
                     /locus_tag="VF226_09670"
     CDS             2675..3100
                     /locus_tag="VF226_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7074296.1"
                     /translation="MRVSIPILLEAAYASWAAQDLDATMACFAEDVVFATHLPADVVP
                     FAGVSRGKVELRRQLQMIIDQFEFLTYRPVRISTEGRLFRSQVKMHYRHRATGLTYKG
                     RLRHVWEIEGDKITHFEEFHDPNRVRAFFQLIALYGPPP"
     gene            3198..3437
                     /locus_tag="VF226_09675"
     CDS             3198..3437
                     /locus_tag="VF226_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074297.1"
                     /translation="MARTFLVIACLWIAGVAYVAGITWPVFPLDMPANDPQVRAAYDR
                     AIWSHLVSYALVAVVPAAILIGFGWSLSKRRREAR"
     gene            3591..4079
                     /locus_tag="VF226_09680"
     CDS             3591..4079
                     /locus_tag="VF226_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074298.1"
                     /translation="MEIRRTLVTHAVVGVAAVLLFAATAARAASPFEILRGTWSGSGN
                     VQFEGGQSERLRCNAYYTNSGGGDVLGLAIRCASASNRIEIRGRLNYHNGNVSGTWEE
                     RTFNASGTATGRASDSRVSLRLAGTIPGTMNVTVSRTRQSVTISSQGSGFRAVRIGLR
                     RR"
     gene            4245..4817
                     /locus_tag="VF226_09685"
     CDS             4245..4817
                     /locus_tag="VF226_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX7074299.1"
                     /translation="MNIQDAKILIMATDGVEQLELTVPRDKLREAGATVHVAAPKNRQ
                     RPDVIRGWDMKDWGQEIPVDKDLEDVNPDEYHALVIPGGQINPDLLRVQQKPVELVRK
                     FYESGKPLAAICHGPWLLIEAGVVKGATATSYHSIRTDMINAGANWKDEPVVVDKGLI
                     TSRSPADLEPFVAKIVEEIGEGRHERRVAA"
     gene            5276..5488
                     /locus_tag="VF226_09690"
     CDS             5276..5488
                     /locus_tag="VF226_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7074300.1"
                     /translation="MRITGTVKFFNTAKGFGFIRPEQGGNDVFVHATALERAGIRSLN
                     EGDRVSFVLEDDRRGRGKQAGQIELA"
     gene            5709..6203
                     /locus_tag="VF226_09695"
     CDS             5709..6203
                     /locus_tag="VF226_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074301.1"
                     /translation="MTHLALAAALAVSPASIAFASDALAQPASPNVFAELDGSWSGRG
                     SVRFSDGKSERITCRAYYNPKSAGAELGLAIRCASVSYKIEIRASLLNRNGRLTGQWE
                     ERTFNASGEVNGRASAGQIKMNIAGGGLTASMSVSTEGSRQAVSISTEGSNLKGVTIN
                     LSRG"
     gene            complement(6297..7004)
                     /locus_tag="VF226_09700"
     CDS             complement(6297..7004)
                     /locus_tag="VF226_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7074302.1"
                     /translation="MESLQTIVRVFRRRGSLSDDELVSLAKERDEDAVRILVRRYNQR
                     LFRIARGIIRDESEAEDVVQATYVRAFTHLDRFRGEAAFSTWLTRIVLNEAYGRVRRK
                     RSTVELSEIDAPAEGGQVIMFPMTPRPTDPESDAGRRQVRELLETALDRLPEAFRMVF
                     ILRELEELSVEETAALLGIKPETVKTRLFRARRLMRTEIEKAVSVNFAELFPFAGSRC
                     ASMADKVIASLRRHARW"
     gene            complement(7022..7504)
                     /locus_tag="VF226_09705"
     CDS             complement(7022..7504)
                     /locus_tag="VF226_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX7074303.1"
                     /translation="MTAALLLGPRVARAADAIEIVMGGRPDGSHVWFDPIGLHIEPGQ
                     TIRWINRDAGNSHTATAYHPDIFGRPRRIPSSASPWDSDYLLPGESFSITLTTPGVYD
                     YYCVPHEHAGMVGRIIVGSPEPPGWTNGPSSEEDLPEAALHAFPTVAEIMSKGLVRRA
                     "
     gene            complement(7540..7938)
                     /locus_tag="VF226_09710"
     CDS             complement(7540..7938)
                     /locus_tag="VF226_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5602 domain-containing protein"
                     /protein_id="HEX7074304.1"
                     /translation="MTLAGVSPSFAHGADKALAKSPPAAPYQQVSKLVKLPDFIPGLG
                     QLFVDPSTLPAGPFLAYDHDGKLVSTIYMIPLNELKPDGKPFNDLKLSGAAVDHVDVH
                     YNAGHPGVETPHAHIVLWHVKSAEESRVAK"
     gene            complement(8203..8964)
                     /locus_tag="VF226_09715"
     CDS             complement(8203..8964)
                     /locus_tag="VF226_09715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074305.1"
                     /translation="MSSSTGKVALVTGAGSGIGRAAALALQRNGYDVVLAGRRKEELE
                     RTASMAVEGGGRMLPVPTDVADPASVKALFAATKENFGRLDVLFNNAGMGAPAVPMEE
                     LPYETWQKVVDVNLTGSFLCAQEAIRIMKAQTPKGGRIINNGSISAHAPRPNSIAYTA
                     TKHAITGLTKSISLDGRAHDIACSQIDIGNADTPMGGRMKHGVPQANGTIAPEPVMDV
                     EHVANAIVFMANLPLDANVQFMTIMATKMPFVGRG"
     gene            9236..9598
                     /locus_tag="VF226_09720"
     CDS             9236..9598
                     /locus_tag="VF226_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074306.1"
                     /translation="MAFSKTLLVAAGIAGCLFAGSAWAGGSRWDRTAYYEGGPIEGHV
                     VAESRFGHGTVSGPVRRSRTGYEVRMPGGTWIACRRSCSETLRVETVDFWENRGAGRN
                     AIDSECGLLGCLRWEWWF"
     gene            complement(9614..10279)
                     /locus_tag="VF226_09725"
     CDS             complement(9614..10279)
                     /locus_tag="VF226_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7074307.1"
                     /translation="MESNSTAQAQRSGLKAAIVPVTPFQQNCTLIWDEATKVGAVVDP
                     GGDLDRIRSAISEVGIKAQKILLTHGHIDHAGGAAELHEELGVPIEGPHQADRFLLDG
                     LAEQGRAYGMAARSVVPDRWLQEGDTVEVAGHSFSVLHCPGHSPGSVVLYNSGQRFAL
                     VGDVLFRGSIGRTDFPYGDHEALINAIKTKLLPLGDDVAFICGHGPTSTIGAERASNP
                     FIT"
     gene            complement(10410..11105)
                     /locus_tag="VF226_09730"
     CDS             complement(10410..11105)
                     /locus_tag="VF226_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7074308.1"
                     /translation="MRVLLIEDDRATAQSIELMLQSEGFNVYTTDLGEDGADLGKLYD
                     YDIILLDLNLPDISGYDVLKTLRVSKVATPILILSGLAGIDDKVRGLGFGADDYMTKP
                     FHKDELVARIHAIVRRSKGHAQSVIETGEVRVNLDTKTVEVNGQRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLQAATGGRHYIETVWGRG
                     YVLRDPNDEHAAA"
     gene            11739..12122
                     /locus_tag="VF226_09735"
     CDS             11739..12122
                     /locus_tag="VF226_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEX7074309.1"
                     /translation="MKSREISFRLKRFAAEEKRRKVADLEHMIREFEHMAIELDRQVQ
                     SEEERTGIRDKTHFAYSTLAKSAAQRRDNLRASIADLQTKLEIAVRERDDACADLDKL
                     AAPETQRDLDRLPRRPGRDPNMTIS"
     gene            complement(12495..12773)
                     /locus_tag="VF226_09740"
     CDS             complement(12495..12773)
                     /locus_tag="VF226_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEX7074310.1"
                     /translation="MAEQQFRSRVRYVIGPDGSPLTIADLPPRDTKRWVIRRKAEVVA
                     AVRGGLLSLEEACERYTLTVDEFLSWQRSIDKHGLPGLRATRIQDYRD"
     gene            13107..14324
                     /gene="mnmA"
                     /locus_tag="VF226_09745"
     CDS             13107..14324
                     /gene="mnmA"
                     /locus_tag="VF226_09745"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7074311.1"
                     /translation="MSAGETTAPSYGGPTTRGARRRVIVAMSGGVDSSVVAALLKREG
                     HDVIGITLQLYDHGVATARKGSCCAGQDIHDARRVAATLGIPHYVLDYERRFERSVIS
                     SFADSYLAGETPIPCVTCNQQIKFRDLLETATELGADVLATGHYIRRCDGPDGPELRR
                     AVDTDRDQSYFLFATTREQLSRLWFPLGGLRKPQVRELASELGLSVASKPDSQDICFV
                     PSGRYADVIERLRPGASEPGDIVHVDGRVLGRHDGIMHYTIGQRRGLKLAGGTPLYVV
                     RLDPERRQVIVGPREALRTDVLELRNVNWLGSRAEAAAARDGLPIRARIRSTHTPVEA
                     TLVLDEALGRARVLLPEGEHGVAPGQACVFYDDAGPEARVLGGGWITRTKRAIDEDAS
                     RTMPRSVAAGLAT"
     gene            14387..15070
                     /locus_tag="VF226_09750"
     CDS             14387..15070
                     /locus_tag="VF226_09750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7074312.1"
                     /translation="MAKQSRRPFGGTLAAKALPNMDESSVISAYRRWAPIYDHTFGKI
                     AAEGRKHAVEIINSRTGRVLEVGVGTGLSLPSYGKHLEIVGIDLSPDMLEKARQRVAN
                     EGLDNVAGLYEMDAGHLTFSDNSFDTVVAMYVMTVVPEPERVMRELARVCKPGGEVIL
                     VNHFSQEEGVRGWVERRMAPFADQLGWRPVFDVDRVLVCDDLEVVERRALRPMGIFTM
                     LRMRKRQLA"
     gene            15190..15266
                     /locus_tag="VF226_09755"
     tRNA            15190..15266
                     /locus_tag="VF226_09755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15224..15226,aa:Met,seq:cat)
     gene            15626..15967
                     /locus_tag="VF226_09760"
     CDS             15626..15967
                     /locus_tag="VF226_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7074313.1"
                     /translation="MKLKTKIDPNAMRSAADEASALLKAMSNRHRMLILCQLIDGEKS
                     VGQLAEFLGIRDATASQHLALLRRDRIISARRDGQTIWYRISSQPALEVMRVLYDIYC
                     AQSKAQPTARQ"
     gene            16152..16355
                     /locus_tag="VF226_09765"
     CDS             16152..16355
                     /locus_tag="VF226_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEX7074314.1"
                     /translation="MTIDRAVMIFAGFMILLSLTLGYYVSPYWYLLTAFVGLNLIQAS
                     LTGFCPAAMVFKKLGCKSGNIFR"
     gene            16413..17429
                     /locus_tag="VF226_09770"
     CDS             16413..17429
                     /locus_tag="VF226_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7074315.1"
                     /translation="MIMFSRAIFLVLSTFLGIAPAAADFVVKSISIPDMKAVFGQVES
                     RRIVPARARIGGSIRAIKVSEGNEVKEGETIAIVVDDKLALEMSAANAKIEALNSQLE
                     NARIELDRAQQLLARGTATQARVDQAKMQFDVVANQLAAAQAEKSVIEQRAREGEVLA
                     PADGRVLTVPVTLGSVVLPGEEVARIASGRYYLRLFLPERHAAGIRQNATVLIGQRGL
                     SPATSGSASEARPGRIVKVYPEISAGRVVADVEVDGIGDYFVNERALVWIPVAERSAF
                     AVPPEAVSTRHGIDYVRIATETGSREVAVILGETIFTADGPRVEVLTGLHDGDRIIMA
                     DRKP"
     gene            17426..20692
                     /locus_tag="VF226_09775"
     CDS             17426..20692
                     /locus_tag="VF226_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7074316.1"
                     /translation="MKLGLAGLLTRGSIASPLTPLFLLAAFALGLVALVTLPREEEPQ
                     ISVPMVDIHVAADGLKAEDAVKLVTEPLETIIKSIDGVEHVYSQTEDDGAVVTARFLV
                     GTDADAAILRVHEKIRANMDRIPFGIPEPLIVGRGIDDVAIVVVTLTPTPAAADRWTA
                     NDLTRLARELQVEVLKLPDIGLTYIVGEQPEEIRIQPDPERLSLYGITLQQLASKIAG
                     ANRSFQSGRVREAGEQRALVAGQTLQTPSEVGNLLLTARDSRPVYVRDVATVILASEP
                     NETRVTSVRKAPGGLERTPAVSLAIAKRPGTNAVVIADAIVERLEQARGQIFPADVEM
                     TVTRNYGETANEKANELLFHLGLATVSIVILVGFAIGWREALVVAVVIPTTILLTLFA
                     ARIMGYTLNRVSLFALIFSIGILVDDAIVVIENIARHWAMKDGRPRVKAAVEAVAEVG
                     NPTIVATLTVVAALLPMLFVSGMMGPYMSPIPTNASAAMLFSFFVAVMVAPWLMMKFG
                     GHAHAQSHDHHAAQGGWLGRAYVKVAEPVLRTKRRSWAFLLAVGAATLASLGLFYTQH
                     VTVKLLPFDNKTDLQVVLDLPKGASIEDTDRALQAMVDELAGVPEVASFQTYAGTAAP
                     FNFNGLVRHYYLRSQPHQGDIAVNLVAKGERTRTSHDIALELRERLKAVKLPEGSVLK
                     VVEPPPGPPVLGTLLAEIYGPDPDTRRAVATKVREAFASVPFIVDVEDSFRNRPERVR
                     IAIDQDNLEYYRVEQADVYDTIRTLAGGTTVGYSHRGGGRSPIPIRIALSKSERVVGE
                     RTLATPVPANALPGDRSIVELGDVVSVTREKASYPIFRHNGRAAEMVMAELAGAFEAP
                     IYGMLAVSDAIAQADWGEVPKPTIALHGQPNDESHPTLLWDGEWEVTWVTFRDMGAAF
                     MVAILGIYILVVAQFGSFKLPLVILTPIPLTFIGIMLGHWLFGAPFTATSMIGFIALA
                     GIIVRNSILLVDFIRHARSPERPLIDVLLKAGAIRFKPILLTALAAMIGAAVILADPI
                     FQGLAISLLFGLMSSTALTVLVIPAIYIVLRSNGSKGNAARGERTGSSQQGEPEIIPT
                     "
     gene            20785..21141
                     /locus_tag="VF226_09780"
     CDS             20785..21141
                     /locus_tag="VF226_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7074317.1"
                     /translation="MQTDWQAFTRELSGGLRDLRGGAPEVMKGFSEIAQAALKANALD
                     TKTKELIALAISVAIRCDDCIGFHAKAAVGQGASREEVLETLGMAIYMGAGPAVMYAS
                     HALQAFDQFEKARPAR"
     gene            complement(21801..23303)
                     /locus_tag="VF226_09785"
     CDS             complement(21801..23303)
                     /locus_tag="VF226_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7074318.1"
                     /translation="MEGLEALFHGFSVAITAHHIILMIIGVLLGILVGVLPGLGAPNG
                     VSLLLPLTFTMDPVAAIILLTSMYWGALFGGSTTSILFNIPGEPSSVATTFDGYPMAK
                     QGRATQALSYAFLSAGFGALLGVIVITLLSGWVARFAMQFSPAEYFAVYFLAFASFVG
                     FGGASPLKTIVAIGTGFTLAAVGMDSVSGNLRLTYGFDQLVAGISFLVAVIGLFGIGE
                     LVITIEDGLRLQGLRARISPRDVLNTALSMPRHWLALLRSGLVGCWMGITPGGPTAAS
                     FMSYGLAKRFSRDRDKFGKGEPEGIISPETADHAAGTCAMLPMLALGVPGSATAAVLL
                     GGLMIWGLNPGPMLFIERPDFVWGLIASMYLGNVVAVILVLSTVPLFAAILRIPFSIF
                     GPAIVVVCLVGAYTVGSKGFDLWLVLVFGVLGYAFKKLDYPIAPLILAMVIGDKAEDT
                     FRQSLIMSKGSLSIFWSNPLVAGLMTLAFILLLSPIAVSFLRSLRKGSVA"
     gene            complement(23313..23789)
                     /locus_tag="VF226_09790"
     CDS             complement(23313..23789)
                     /locus_tag="VF226_09790"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7074319.1"
                     /translation="MISRFGAELVMAIIVAVFGLIITLGALEFGVGWSPSGPEPGTFP
                     FYVGLIVTVAGVGIIFQTLIQRAYRDESFLTSAQLRSVIFFSLPIVALVAVSLWVGLY
                     VGMALYLFASMKFQGGYRAGFSALVAIGMAVSLYLILEIGFQTPLLKGPLEDALGL"
     gene            complement(23857..24852)
                     /locus_tag="VF226_09795"
     CDS             complement(23857..24852)
                     /locus_tag="VF226_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7074320.1"
                     /translation="MLHVRQLIGIAAAVSMLGVPAAHAWEPTKSIEFVVTAGAGGGTD
                     NFARVVQSAITKNKLLDEAIIVTNKGGGSGAEGYIYARGAAGDPHKVIFGTNNAYLLP
                     YVANVAFKMSDLTPVAAMALDEFLLWVNAGSEYKTAKDYIAAAKAKPETLKMGGSQSK
                     DTDQTLVSLIESATGAKFIYVPFNGGGLAAVQLAGGHIDSNTNNPNENIGQWKAGQVR
                     PLCVFSPTRLPEGPKVTGDMGWSDIPLCKDEGIPIDSFQMPRTIWLPANVPADAVAFY
                     TELFKKVSETPEWKEYVTRTVQTARFMSGDELAKFIADDEAKNKQVFEKEGWLVK"
     gene            complement(24928..25965)
                     /locus_tag="VF226_09800"
     CDS             complement(24928..25965)
                     /locus_tag="VF226_09800"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX7074321.1"
                     /translation="MQSGSGDVQPTIAFAIGDPAGISPELSARLIADPEIRAKARLVV
                     FGDKRILEMGAAVAGVDPDVHVVTAEDAIPADATRPIFVDLRSLDPADVTPRTATVAG
                     GKFASENFRRALQLAAAGKAGAVFFTPFNKAAMRLAYPGYDDEIRFVRDVTGYDGPAS
                     EFNILGKLWNARVTSHIPLSKVASAITQERILGSLELTHSSLRAAGFTHPRIAVAGLN
                     PHAGDGGIFGREEIDIIGPAVTKAQQMGIDAKGPYPSDTVFLRARKGDFDAVLTMYHD
                     QGQIAMKLMGFDRGVTLIGGLPFPICTPAHGTAYDIAGKGIADLGASRTALLLAIEMA
                     GNRHRPDAKAA"
     gene            26118..26819
                     /locus_tag="VF226_09805"
     CDS             26118..26819
                     /locus_tag="VF226_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7074322.1"
                     /translation="MSEDPSGDIRPSAADGMEGMLPIARRTLHDEVVERLRDLIIQGR
                     LAPGTRINEVQVGALLGVSRTPLREAIKTLAREGLVENVPSRGAVVRRFSEADVAQIH
                     EVLKTLEQLAARLACERADDETITHIHRLHQRMLEFYRNGNRLEYFKLNQAIHTAIVR
                     ASGNAALAETHELLQARIRRVRYTGNDTPSQWAGAVAEHEEMIEALIARDGERLANVM
                     ARHLDETMSRVRDAL"
     gene            complement(26857..28647)
                     /locus_tag="VF226_09810"
     CDS             complement(26857..28647)
                     /locus_tag="VF226_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7074323.1"
                     /translation="MARKLKKVLERETELDQPGLAHLREAVANCARKRAPGPPRPWHD
                     GIAGLNTAISSVPDGMANGLLVGVNPVYGLYACILGPIVGGLFSSTHLMVTTTTSAVS
                     LMAGQVLSALSAQERDVILVVLVVLVGLAQISFGLLRMGRLTRFVSYSVMAGFLAGIS
                     VLLVLSQLPNATGYEPIGQNRVTQTLDLLANLDQVHLPTLGLAALTLFLALVLPNIGL
                     GSIGTFIAILVPSGVTYLFGLDVKLVRNIGEIGNGFPDFEVPSPFDVTFELVTGALAI
                     AAVALVQSSGISQSVPNPDGSRSRASRDFVVQGTANVASGFLHGIPVGGSLGATAINV
                     ASGARTRWSVIFAGVWMAALVVILAGPISYIALPSLAGILIFAGIKAIRPMDIRLIWQ
                     VGGPPRLVTASTFIATLFLPLQAAVGFGAVLSALIYIYRSSIDVLLVEQYLRPDGEIA
                     ERKPAKRLPSNAVTVLYVYGHLFYAGAAILSRQLPRPNGAQNPVVILRLRGQPNVGAT
                     LAHVLSRYAAELNARNGRLYVTGLHRRTFEQLVEEGRLRLSDPIRGYELTPIIGESTR
                     EAIEDARAWLVRQTEQESRRERRPDDSGAG"
     gene            complement(28716..29108)
                     /locus_tag="VF226_09815"
     CDS             complement(28716..29108)
                     /locus_tag="VF226_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7074324.1"
                     /translation="MLGSILSRTVNGSALFLPTMVAGLVLVGLHWLFAFIAYHTSIFG
                     PVVKGEPVLLIKDGVVQEDGMQSVNLTPRDLNEALRLQSGHTDPKRIRRAYMERNGNI
                     SVVPYENETRVLDVKVEEGVQTIRIKLE"
     gene            complement(29400..31094)
                     /locus_tag="VF226_09820"
     CDS             complement(29400..31094)
                     /locus_tag="VF226_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7074325.1"
                     /translation="MARTKRKTRNDRPNFLILCMDQWDTHMRAPKEVEFPAMQRLKAE
                     GVSFDRQYCAVPLCTPSRAAMWTGVHAKQVGLSDNTNFAWIDELSQDFPTIGHLLREQ
                     GYYTAFKGKWHVSAVPKSEDALERYGFSDYQQWGEMFGAPLQGAMLDGAATFETIDWL
                     EHKRAELDQPWLLICSLINPHDIMFLQTDPIEAPDENGMIKGLQSTVQRLGWFEKEWP
                     VEMPDNFDDDYRLQPFGVHHYKEFIDQNYGRVPDERTDLWLKRRNYLINCMRLVDAEF
                     SKVLAAMDRLDLWKDTIVIFLSDHGEMNCAHRMTQKGAIHFDEATIVNLTVCVPGGPK
                     GKRTIAVGSHLDLVPTILEFAGLAPDEVKARYPHLKGRSLRSAIMNPQSHGPRGSADV
                     PGDGALVCWDALHSLDVNWALSGALQALTDLDEGPLGKADPKQVRRLYGAPDFTNRTF
                     FRAVVDGRYKMVRWFSPLEYDLPFTLGELYARSDVTLHDLVKDPGEMENIGHPDHPGY
                     DPVIVERMLDKMNALIQHEIGEDRVPFDLDLFGTRKVIYSKPRKARPRRVKAKAKT"
     gene            complement(31126..31953)
                     /locus_tag="VF226_09825"
     CDS             complement(31126..31953)
                     /locus_tag="VF226_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HEX7074326.1"
                     /translation="MLSVGFGYTVQEIIDPFRDAGKVARALIANFVLVPILALAIVHW
                     LSLDEPIAIGLMLLGMAGGAPFLIKLTEAADHDVGLAASLLVLLLPFTILYLPTMMPL
                     VVPAVTVDAAAIAKPLVLAMLLPLGIGLLARAWLPRSAQRLRPLMGTLGSIALVAMVS
                     FTLIVNFREVVGLFGSGAILAATILTAGAYAIGFLLGGRDPASHNILALGTGQRNVSA
                     ATVVATQSIDDPDTLATVIVSSVIGIAILFPTAWRLQRKRADTHIRDLYDRRLTGRG"
     gene            complement(32475..32747)
                     /locus_tag="VF226_09830"
     CDS             complement(32475..32747)
                     /locus_tag="VF226_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7074327.1"
                     /translation="MGPSTSANGIAYPQLAAIFEDVFQYSGPLAPSTSPDDVSRWDSL
                     QHIALVRCLEETFAIQLSMDEMMEIRTVADIENVLRRHGVCEQRGS"
     gene            complement(32748..34967)
                     /locus_tag="VF226_09835"
     CDS             complement(32748..34967)
                     /locus_tag="VF226_09835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEX7074328.1"
                     /translation="MSLSDAHQGADRPRETGARTPTTADQLVEAAIASAADCLRAGVL
                     LQGVDWTRVAPDRVGTMLQDLEAHHVRVPSWLSRALVLAGHVLPGELVRALPEELAAL
                     DELNRATGSAAERRAFAAIHRRLCHRESGDGDVLAAIVKRLQALGWNDEAAELALSVH
                     DVAPRVLTSAAAPLDHYRQGLQIARIRLCGFSTTHTLAQDLVPAFAAQGWQARVSEAN
                     FGEGLAELLRPAADCDGLVLLMDFDGFAARDWRRPADEGLELVRERADLLASALTAFA
                     ERAQHPLLINTIPSAPAPTAGLLDRRHALGLRGAIDLVNQRILDAAERSGRIVVCDAD
                     SALAKLPLAQQTDPKLWYYGRIAYSAAASRALARRFAEAWHLLKRGPLKVLALDFDNT
                     LWGGVYGDDGLGALVCGPEFPGNAFSTFQQECLRLKRQGMLLAALSKNDPDAITVFDR
                     HPGMVLRADDFAATAIDWNPKPENIRKLAAELNLGLDSFLFLDDSPHEREAMRQVCPE
                     VVVPEMPADPAQRPLWLRSLAVTWPVRLTAEDERRAEMYAAERASRQFRAQAVSLSDY
                     LRGLEQRLHVGPVRSATLSRVAQLHQRTNQFNLTTVRYTEADLGAMIADEQHYLVLQG
                     RASDRFGNHGIVIAATARIDGAAAEILTFLMSCRVIGREIERAFMGALLEELGRRGVR
                     EVTGTYVPSAKNGQVRDLYSSLGFREVSTGPSQTTWSWTIGKAGLPGSNFVVVQWET"
     gene            complement(34977..36191)
                     /locus_tag="VF226_09840"
     CDS             complement(34977..36191)
                     /locus_tag="VF226_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074329.1"
                     /translation="MTGSQDRLLELQVGQTESVSRTITSDDVAAFARLSGDYNALHLD
                     EEFAARTEFAQRVVHGFLHASLLSTLIGTKLPGRGALYTSQTIEFTRPVFIGDTVEAR
                     ATVEKIDRDTRLVTLCTEILNQHGECVLRGRAQVKVLRLAEDSHPEPESTPRGEGLLA
                     GRKALVTGASRGIGRAVARLLARHGAHVWINYYKSSAAADDLAAEISAAGGRCRIVRA
                     DVTDQHDVDRLLAAVADDGRLDILVNNAGPKIHSASFDRLGWDDMSSAHDAIVGSAFR
                     VTQAALPALKASRGVIVNVLTSAALGRTAHHWLPYVTGKAALLAMSKNLAQELGPQGV
                     RVNMVSPSMVDTDLVANVPDRVRQMVVARTPLRRLATTEDVAGAVLMLVSPYAAFITG
                     ENLLVTGGEVMV"
     gene            36392..36670
                     /locus_tag="VF226_09845"
     CDS             36392..36670
                     /locus_tag="VF226_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7074330.1"
                     /translation="MPSTFETVAGIIAATSDIPIEQITPDSHVMKDLEIDSLAFLDIT
                     FEIDRRFGIEVPLDRWLEQVNEGKVKSEDYFVLRNLCAQIDSLRAAAA"
     gene            36686..37156
                     /locus_tag="VF226_09850"
     CDS             36686..37156
                     /locus_tag="VF226_09850"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HEX7074331.1"
                     /translation="MLLERFQMLDRVDAFDREGASLRAFASVPEESPIFEGHFPGHAL
                     MPGVLLLEAMAQASGYLLLALGGFSRMPFFANVKSANFRSFVLPGDQLVIEAERVHAG
                     SGYAVMSSQILRGGTRVSDAQMTFRQVPFPVPALEQHVRREGERLGFFGLADSR"
     gene            37153..38373
                     /locus_tag="VF226_09855"
     CDS             37153..38373
                     /locus_tag="VF226_09855"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX7074332.1"
                     /translation="MSALPHQVWITGIGLVSSLGEGVEAHWECLTGSAQPRPVVDETR
                     FAPYPVHPLTKLDIARQIPKTSDQRQMEQWQRTGVYAAGLALTDAGIAGDTELLDRTD
                     VVTAAGNGERDLELDARVLETIGGEAQPDVYLNKALATGLRPTLYLGQLSNLLAGNIS
                     IIHKARGSSRTFKGEEMAGVAAVENAVRRIAHGESDVVLVGAALNAEREDLLLGYELD
                     GSLLRGPFRSVWVRDANGGFVPGSIGVFLVLESELHGAFRGATPYAAITGIASDRARR
                     EAEPVSNTAMELFDRIVDGLRPGPLPVLSGASGVEPITSQERAFLKQLESRDWPLAVR
                     AYGSVLGHSVEAHFITGVALAAMAVSRRSFFAPFDASDVERPFQGVPRRVLVTGFGHW
                     RGEGLALLEAADWD"
     gene            38375..39655
                     /locus_tag="VF226_09860"
     CDS             38375..39655
                     /locus_tag="VF226_09860"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX7074333.1"
                     /translation="MARPIRDHLGRRVVVVTGMGLITALGRGKAENWAGVIAGRSGLK
                     RISRFPTENLRSTVAGTVDFMDVNPYSAHDLSLAMAIATAEEALAEARIESRAFPGPL
                     IVATPPSEFEWPLLRKLHDESPEPERSGYARLLAAARTGNYRDMARHLRFASIADRLK
                     ERFGCRGEPLSICTACASGTTTIQLGLEAIRRGEADAALCIGTDATVHPEGLIRFSLL
                     SALSTRNDPPEKASRPFSKDRDGFVIAEGSGALVLEAYESARARGAPILAVVRGCGEK
                     ADDYHRTRSRPDGTAIIGAIQKTLDDAGVAPDEIDYINAHGTSTPENDRMEYLSLKAV
                     LGDRLHQVPISSNKSMVGHTLIAAGSVEAVFSVLTLQNGLLPPTINYDIPDPEIPLDV
                     VPNEARAAKVRTVLSNSFGFGGQNACVVFSGEPE"
     gene            39709..40734
                     /locus_tag="VF226_09865"
     CDS             39709..40734
                     /locus_tag="VF226_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7074334.1"
                     /translation="MRAIEVRGERQLAIIETERPPAPGPGEVQVRIGAVALNHIDVWG
                     WRGMAFARRRLPLIAGAEAAGEIVKVGPAVEQLRPGDIVALYGAHTCGICPACREGRD
                     NFCTAQDNIYGFHIDGFARELMNIPARLAVRAPYGVDVTGAALAAITFGTPEHMLFDN
                     ARLREGETVLVHAGGSGIGSAAIQLAKAAGATVITTVGSDEKAAKAKALGADHVINYR
                     EERFERRVRQITGRRGVEVVFEHVGSDTWEGSMLSMKRGGRLVTCGSTTGISAKINLF
                     MLFQQQLRLIGSFGCTMANMRSAMAKMAQRQVRPVIDTVIPIGQIERGLQRLESRQVF
                     GKIILTL"
     gene            40755..41987
                     /locus_tag="VF226_09870"
     CDS             40755..41987
                     /locus_tag="VF226_09870"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX7074335.1"
                     /translation="MPGNPREVWVTGIGLVSSLGEGIEAHAALLSPAARPAPILNESA
                     YAPYSVHPLAPLDLSKQIPKKSDQRQMEGWQRIGVYAAGLALADAGIAGNAELLDRTG
                     LVVAAGSGERDTAVDCTVLEKLDGREDTGVLAKEVLPSALRPTLFLAQLSNLLAGNIS
                     VVHNVTGSSRTFMGEEMAGLAAAENAVRRIAAGQGDLFLVGGSLNAEREDLLLGYELG
                     HNLWRGPFRPVWERHEAGGGFVPGSVGAFLVLEARGHAEARGARPYAKVTGVVSDRCN
                     RTHGEAAELGLRLFATLCSGLSDGPLPVLSGASGVAPVTQQELDFLRALDSGGFDPII
                     RAYGSLVGHGVEAHLATGMALAALTISRQNFLPPLDDSGVERPFEGDPGRVLVTSFGH
                     WRGEGLALIEAVAEGGTR"
     gene            41984..43249
                     /locus_tag="VF226_09875"
     CDS             41984..43249
                     /locus_tag="VF226_09875"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX7074336.1"
                     /translation="MRDSKGRPVVVVTGTGVVTSLGLGKAENWAKLTSGCSGIRRISR
                     FPTDGLRTTIAGTVDFLDVTPFSAPALTMAMAAAAAEEALAEAEIGEKGAFPGPLFLA
                     TPPAELEWPQRRLLWEAHAGGELTGYKRLLAAARESRHADLQEKFQFASVAEHLACRF
                     GTRGLPVSVSTACASGATAIQLGVEAIRRGETNAALCIGTDSSVQLEALIRFSLLSAL
                     STRNDPPEAASRPFSKDRDGFVMAEGAGALVLESYERARARGAQILGVVKGCGEKADD
                     YHRTRSRPDGTAIIGAIRNAIEDAGIAPHEIDYINAHGTSTPENDKMEYLSLKAVLGD
                     HLEKTPISSNKSMIGHTLSAAGAIEAVFSLLTIRTGVVPPTINYRNPDPAIPLDVVPN
                     EARRATVRTVLSNSFGFGGQNASLILAAL"
     gene            43262..44206
                     /locus_tag="VF226_09880"
     CDS             43262..44206
                     /locus_tag="VF226_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074337.1"
                     /translation="MIASQWHFRVIAWRGEAHPRSRSASSSDKVGAVVALAALAAVCA
                     TSTLPVIEAKQEAPEAAVARTGPLTDRETFIGAYTGAPYTYSSNLHFQRPDGSELTIN
                     DVAWDGKPFDDPIYYGARVIRWTAMSRIGSMFDFTHGKAYSQPEQEVSVTGKGEPETK
                     KISDIFHKLEFTHGHNMLTLNGLFRLPWGTSAIKPYVGLGAGISVPHTEVQFRGERGR
                     TYEYQFTGPVLQVVGGLELRVPRLSYFVEYKFTIAPYRAPLHLRDGSLAFADLWHQFL
                     RWWEGSEPAGGWLTTRLTSHQVIAGMGYRSGNVLAAGP"
     gene            complement(44279..45784)
                     /locus_tag="VF226_09885"
     CDS             complement(44279..45784)
                     /locus_tag="VF226_09885"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX7074338.1"
                     /translation="MSLRSLPPFPDLRAFIAHLDRSGQLMRVDEPVSLVHEMTEVHRR
                     MLRAGGPALLFERAVMADGRTSDIPVLVNLFGTRERVAMGLGVTPDGLDALGEAMAWL
                     RQPEAPSDFRDAFRRWPLLRAALATRSVAARSAPVQEVVCQGDRVHLSTLPIQTCWPG
                     EPAPLITWPLVVTSPPGDYAPGRTNVGVYRLQVLGRDRLIVRWLANRGGARHHAQWRA
                     KGEAMPIAIAIGADPALMLAAAMPIPEAMSELTFSGLLRGRSTRVAPGITVPVPVPAD
                     AEIVIEGTVSPGETAPEGPYGDHTGYYNSVEPFPVMQVTAITHRRAPIYLSTHTGRPP
                     DEPSVIGEVFARLLVPLLRKQFPEIEDFHLPPEACSYRMAVVSIRKRYAGQARRLMLG
                     LWSALPQFTLTKMIVVVDPDIDVRNWADVIWAISTRADLARDLVTIADTPIDYLDFAS
                     PKPGLGGKLGIDATNKIGPETEREWGRVLTMSPEVVQRVDAIWHRLTTGRG"
     gene            45881..46420
                     /locus_tag="VF226_09890"
     CDS             45881..46420
                     /locus_tag="VF226_09890"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX7074339.1"
                     /translation="MPNAPQLPALLTSAMRLLPTQPLELALDRLTKSIAQRHPDLFDR
                     LGEHSHKAIGIAPTDVPVIFVLMPDREAPRVEVKRTDSAPACDASIVGPLFALVDMAE
                     GRLDGDALFFSRDIRIEGDVEAVLALRNALDGEGLDLIREATAPLGILAAGPELIGRR
                     CLNLLRSALQTAPAEETPF"
     gene            46417..47412
                     /locus_tag="VF226_09895"
     CDS             46417..47412
                     /locus_tag="VF226_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX7074340.1"
                     /translation="MKPQSRLELVCPAGTPGALRVAVDAGADAVYCGFRDETNARNFP
                     GLNFSREELKEAVTYAHARGCKVLVAINTFPRAGHVALWQRAVDDAVALGADALILCD
                     IGLLDYAATKHPKARLHLSVQAAAANPEAIRFYTASFGVKRVVLPRVLTVSEIASINE
                     GIDCETEVFVFGGLCVMAEGRCSLSSYATGKSPNMNGVCSPPSHVSYHEEGDELVPRL
                     GGFTINRIPRSEPAAYPTLCKGRFVAGKSSGYIFEDPVSLNAGTLVPGLAKAGVTALK
                     IEGRQRGRAYIAAVVKAFRQAVDALAEGRSVDLDRLLALAEGQSSTQGAYAKTWR"
     gene            47431..>47591
                     /locus_tag="VF226_09900"
     CDS             47431..>47591
                     /locus_tag="VF226_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7074341.1"
                     /translation="MRITLGPVLFNWQPEAWRDFYARIADEAPVDQVYLGEVVCSKRM
                     PFFDELIVDV"
BASE COUNT         8803 a        15118 c        15198 g         8472 t
ORIGIN      
        1 gagagcatga cggcggtgct ggaggcgctg ctggcgcggc cggagcagcg caccgacaag
       61 ggctgggccc tgctcgccca agagccgatg atcgcgagcc atccgctcgt gtccggcagc
      121 atgccgatgt tcatgccgca ccgtccgccg cggccggaga agtcggaagg cggcatcccc
      181 ttccgcctcg tctcggagtt cgagccccaa ggcgaccagc cccaggcgat cgcggagctc
      241 gtggaaggcg tgaaccagca cgagcgcgac caggtgctgc tcggcgtgac cggctcgggc
      301 aagacgttta cgatggccaa cgtcatcgca cggacgcagc ggccggcgct gatcctggcg
      361 ccgaacaaga ctctggcggc ccagctctac ggcgagttca agagcttctt cccggagaac
      421 gcggtcgaat acttcgtcag ctactacgat tactaccagc cggaagccta cgtgccgcgc
      481 accgacacgt acatcgagaa agaaagctcg atcaacgagc agatcgaccg catgcgccac
      541 gcggcaaccc gcgctctgct cgagcgcgac gacgttatca tcgtcgcctc tgtatcgtgc
      601 atctatggta tcggctcggt cgagacctac accgcgatga ccttcacgat ccgtgtcggc
      661 gagcggctcg accagcgcca gctgctggcc gacctcgtgg ccctgcacta ccgtcgcagc
      721 gaccagaact tcatccgcgg cacgttccgc gtgcgcggcg atacggtcga gctgttcccg
      781 gcccacttgg aggaccgcgc ctggcgcatc tcgctgttcg gcgacgagat cgagagcatc
      841 accgagttcg accccctcac gggccagaag accgacgagc tgcagctcgt gaaggtctac
      901 gccaactcgc actatgtgac gccgaagccg acgatgcagc aggcgctcaa agctatcaag
      961 gcagagctga agcagcggct ggacgagttg tacagcgccg ggaagttgct ggaggcgcag
     1021 cggctggagc agcgcacgct gttcgacatg gagatgatgg aggcgaccgg ctcctgcgcg
     1081 gggatcgaga actactcgcg ctatctcacg ggccgcaagc ccggcgagcc gccgccgacg
     1141 ctgttcgagt atttgcccga caatgcgctc gtgttcgtgg acgaaagcca cgtcacggtt
     1201 ccgcagatcg gcgggatgtt ccgcggcgac taccggcgca aggcgacgct ggccgagtac
     1261 ggcttccgcc tcccctcctg catggataac cgcccgctcc gcttcgagga gtgggacgcc
     1321 atgcggccgc agaccatcta cgtctcggcc acgccgggga gctgggagct cgagcagacg
     1381 ggcggcgcct tcgtggagca ggtgatccgg ccgaccggcc tcaccgatcc ggaagtcatc
     1441 gtgcggcccg ccaggaacca ggtcgacgac ctgctggacg aggtgcggaa tgtgacgcgc
     1501 gccggctacc gcacgctggt gaccaccctc accaagcgca tggccgagga tctcaccgag
     1561 tacatgcacg agcagggaat ccgcgtgcgc tacatgcact cggacatcga gacgctggag
     1621 cgcatcgaga tcatccgcga cctgcggctc ggcgccttcg acgtcctgat cggcatcaac
     1681 ctgctgcgcg aggggctgga catccccgaa tgcggcctgg tcgccattct cgatgcggac
     1741 aaggaaggat tcctgcgctc cgagacgtcg ctgatccaga ccatcggccg cgccgcgcgt
     1801 aatgtggatg ggcgcgtgat cctctacgcc gaccagatga cgggctcgct ggagcgggcc
     1861 atcgccgaga cgaaccgccg tcgcgaaaag cagaaggcgt ggaacgaagc gcacggcatc
     1921 acgcctgaga gcgtgcgcaa gaacatcgcc gacgtgatgg cctcgatcta cgagcgggac
     1981 cacgtcaccg tcgacgccgg tctggccgaa gcgggccagg tgctcgtcgg gcacaacctc
     2041 caggccgtca ttgccgatct cgagaagcgg atgcgcgagg ccgcggccga tctcgagttc
     2101 gagacggcag cgcggctgcg cgacgagatc aagcgcctgc gcgagacgga gcttgccatt
     2161 gcggacgatc cgcttgcccg gcaggcggat atcgaggaga gggctggccg gttcgagggc
     2221 aatcgcaaat atgggggtgc cccgtccgca cgcgaatccg cgcggaacga aggcaagccg
     2281 ggctcgcgca tctctcgtga atacgagccg gttcagccca cctacgcgca gtccacctcc
     2341 cggccgcaca agccatcgct cgacgacatg gggccgggga cggaccggcc tctgccggcg
     2401 cgcagcccgc gtgtcgatcc ccgcacgcgc gctggagcct tcggcgaggc tattcgggga
     2461 ccgcacaagc cgacgctcga tgagatgggg cctcatgccg agcgcggatt gccagccggc
     2521 aaaacggcgc cgccgaagcc catcaagacg ttggagctgc cggacgaaac ggagcggaag
     2581 cgccgccgcg gccggccacg gaaaaccggc cggcccgggc agtagtcagc gctcatactt
     2641 ctgatatgct cgcatgtgca ggggggcgat gcacatgcgg gtcagcatac ccattctcct
     2701 cgaggctgca tatgcgtcct gggccgcgca ggacttggac gccaccatgg cctgctttgc
     2761 cgaagacgtc gtatttgcga cccatttgcc ggcagacgtg gtgccgttcg caggagtgtc
     2821 tcgcggaaag gtagagctcc ggcgccagct gcagatgatc atcgaccagt tcgagtttct
     2881 gacgtaccgg cccgtgcgga tcagtacgga agggcgcctg ttccgttccc aagtgaagat
     2941 gcactaccgg cacagggcga cgggactgac ctacaagggg cgcttgcggc acgtctggga
     3001 gatcgagggg gacaagatca ctcatttcga ggagtttcac gatcccaacc gcgtacgcgc
     3061 cttcttccag ctgattgcac tttacggtcc gccgccgtga cctcgtcgta aacgacagcg
     3121 ggaagcggca acttttcagc gcgcgtccaa tgccgtactc tggcaatggg gacttcggtc
     3181 gacacgtgga gcgcgccgtg gcacggactt tcctggtcat cgcctgtctg tggatcgccg
     3241 gcgtcgctta tgtggccggc atcacgtggc cggtctttcc gctcgacatg ccggcgaatg
     3301 atccgcaggt acgggccgcc tatgacagag ccatctggag ccatctcgtc tcgtatgcgc
     3361 tggtcgccgt cgtgcctgca gcgattctga tcggcttcgg ctggagcctg tcgaagcgca
     3421 ggcgagaagc acgctgagct ccgattttgg cgtgtccttc cgggctcagc gcacaagtaa
     3481 atgcgcgccc gatgctttcc gaagattgcg gaagtttcga ttcgtattgc aggaacgcag
     3541 atcgccgatc gagcgttcgt gcctgcggat cacccctagg gaggcccaga atggagatca
     3601 gacgaaccct cgtcacccac gccgtggtcg gggtggcagc cgtgctcctg tttgccgcta
     3661 cggcggctcg ggcagcaagt ccgttcgaga tccttcgggg cacctggtcg gggagcggta
     3721 acgtccagtt cgaggggggc cagtcggagc ggctgcgctg caatgcgtac tacacgaact
     3781 cgggcggtgg cgatgtgctg ggcctcgcga tccgctgcgc cagcgcctcc aaccggatcg
     3841 agatccgtgg gcgattgaac taccacaacg ggaatgtcag cggcacgtgg gaggagcgca
     3901 cgttcaacgc cagcggcact gcaacggggc gcgctagcga tagccgcgta tcgctgcgtc
     3961 tggcgggcac tataccgggc acgatgaacg tcaccgtctc ccgtacgcgc cagagtgtga
     4021 cgatcagctc gcagggctcg ggattcagag ccgtgcggat cgggctgaga cggcgctgac
     4081 gcgaccgcga tcgctgacgc ggctctcaat ccttcaaccc gtctcgatgt cggcaaggac
     4141 gcggggtgct gtttaccgcc ccgagcgcgc gcccatgacg ccgcaacggt ctcgacgcga
     4201 tcgcgttgac cgcaagaagc cccatcaaga agaggagacg cgtcatgaac atccaggacg
     4261 caaaaattct cataatggcg accgacggcg tcgagcagtt ggaactgacg gtaccgcgtg
     4321 acaagctgcg cgaggcgggt gcgacggttc atgtcgctgc gccaaagaac cgccagcgtc
     4381 cagacgtaat tcgcggctgg gacatgaagg actggggcca ggaaatccca gtcgacaagg
     4441 atctggaaga cgtcaacccg gacgaatacc acgccctggt cattcctggc ggccagatca
     4501 acccggatct gctgcgggtg cagcagaagc cggtggagct ggtacggaag ttctacgagt
     4561 ccggaaagcc gttggccgcg atctgccacg gtccctggct cctgatcgaa gccggtgtcg
     4621 tgaagggtgc gacggccacg tcctaccatt caattcgcac cgacatgatc aacgccggag
     4681 cgaactggaa ggacgagccg gtggtggtgg acaaagggct gatcaccagc cgtagcccag
     4741 cggacctcga gccgttcgtc gccaagatcg tcgaggaaat cggcgagggc cggcacgaac
     4801 ggcgtgtcgc tgcctgagcc cggtggaggc gcttgcagcg ctgctgcagc gccccgccgc
     4861 ggggtagccc cgagtaggaa aattgcgagg catcgccggg aaccaaacag cccctctcac
     4921 gtttcacgcc ggtgggcggg cacgatggtg tgcgcgtccc gcaggcggtc tgaccgggat
     4981 atccgtccag acctcggctc tcacagcgtc ggttcaagaa cgggggatgt ggcgtgatgg
     5041 actgggttgg cctctgcggc acccgcgaca tacgacttga tttcccggcg cgaacacgtc
     5101 atatgagcct tgccggtgcc cgctgctgtg gcgtgcgccc tcacgattta cggatagctg
     5161 cgcgaccctc accggccgtt tctttcccga catctgagat ctctgggtct ttttctgcgt
     5221 gggacccgat gactggcttt ctgcacgcgt ttgaacggga agaaggtact acccaatgcg
     5281 aatcacaggc accgtgaagt tcttcaacac ggccaaaggt tttggtttca tccggcctga
     5341 gcagggcggc aacgacgtgt tcgtgcacgc gactgctctg gagcgcgccg gcatccgctc
     5401 gctcaacgag ggcgatcgcg tgagcttcgt gctcgaggat gatcggcgcg gccgcggcaa
     5461 gcaggcgggc cagatcgagc tcgcctgacg accggggcac acccgccgaa agtcggaacg
     5521 ccgctggttt gcgccagcgg cgtttttcgt cggggcggag ggttccaccg gtcgaacttt
     5581 ccgctaacag aatgataacg ccctgttgcc aatttgggac aatccgatct tgccggctgc
     5641 ccgtccggcg tccaaaatat ggtccgagaa ttgccagccc aagggatttc gctgtgacgc
     5701 gcctgcagat gactcatctt gccctggcag ctgccctcgc cgtcagccct gcatcgatcg
     5761 cattcgcaag tgatgcattg gcccagccgg cgtcgccaaa cgtgttcgct gagcttgacg
     5821 gaagttggag cgggcgcggc agcgtgcgct tttccgacgg caagtctgag cggatcacct
     5881 gccgggctta ttacaacccg aagagcgccg gtgccgagtt ggggctcgcg atccgctgtg
     5941 cctcggtgag ctacaagatc gagatccgtg caagcctgct caaccgaaac gggcggctga
     6001 ccggccaatg ggaggagcgc accttcaatg cttcgggcga ggtgaacggc agggcatcgg
     6061 ccggccagat caagatgaac atcgcaggtg gcgggctgac ggcgtccatg tctgtttcga
     6121 ccgagggttc gcggcaggcc gtttccatct cgaccgaggg gtccaacctc aaaggggtga
     6181 cgatcaacct gtcgcgcggc tgacgacgtc gagcgtcgct caggcgaacg gttcgaggcg
     6241 gaggacgcgc gtcactccgc cgcgatcatc tatggtcgga ccacggcgcg tcccgcctac
     6301 cagcgcgcgt gccttctcaa gctcgcaatc actttgtccg ccatgctggc gcagcggctg
     6361 cctgcgaacg gaaacagctc ggcgaagttg accgaaacag ccttttcgat ctcggtccgc
     6421 atcagccgcc gtgcccggaa cagtctggtt ttcaccgtct caggcttgat gccgagcagt
     6481 gccgcagtct cctcgacact gagttcttcc agctcccgca gaatgaaaac catgcggaac
     6541 gcctcgggca gccggtcgag cgcagtctcc agcaactccc ggacctgtcg ccggccggca
     6601 tcgctctccg gatcggtcgg ccggggcgtc atgggaaaca tgatgacctg tcctccttca
     6661 gccggggcat cgatctcgga gagctcgacc gtcgaacgct ttcggcgcac ccggccgtag
     6721 gcctcgttca aaactatgcg cgtcagccag gtcgagaatg ccgcttcgcc gcggaagcga
     6781 tcgaggtgcg tgaacgcgcg cacgtacgtc gcctggacga catcctcagc ttcggactcg
     6841 tcacggatga tgccgcgtgc aatgcggaac aggcgttggt tgtagcgccg aaccaggatg
     6901 cggacggcat cctcgtcgcg ctccttggcg agggacacca gctcgtcgtc gctgagagac
     6961 ccgcggcgcc ggaatacgcg aacgatcgtc tgcaaggatt ccatggctca taacccaatt
     7021 ttcaggctcg ccggacgagc cctttcgaca tgatctcggc gactgtcgga aaggcatgca
     7081 atgcggcttc gggcaggtct tcttccgatg aaggcccgtt cgtccagcca ggcggctccg
     7141 gtgaccccac gatgatcctc cctaccatgc cggcgtgttc gtgcgggacg cagtagtagt
     7201 cgtacacgcc cggcgtggtc agtgtgatgg aaaaggactc gcctggcagc aggtaatccg
     7261 agtcccatgg gctagcggac gatgggatac ggcgcggccg cccgaaaatg tccggatgat
     7321 acgcggtcgc cgtatgcgaa ttgccggcat cgcggttgat ccagcgaatg gtctgcccgg
     7381 gctcgatgtg gagcccgatc ggatcgaacc agacgtgcga gccatccggg cggccaccca
     7441 tcacgatctc tatcgcatcg gcagcccgtg ctaccctggg cccgagcagc agggcggccg
     7501 tcaagccgcc ccccgtttgc agtatccggc gccgggaaat catttggcca cccggctttc
     7561 ttcggcggac ttcacgtgcc agagcacgat gtgggcgtgc ggcgtttcaa cacccggatg
     7621 gccggcgttg tagtgcacgt ccacgtggtc gaccgcagcg ccggacagtt tgaggtcgtt
     7681 gaagggcttg ccgtcgggtt tcagctcgtt cagcgggatc atgtagatcg tgctgaccag
     7741 cttgccgtcg tggtcgtagg caaggaacgg gcccgcgggc aacgtcgacg gatcgacgaa
     7801 cagctggccg aggcccggta tgaaatccgg cagcttcacc agcttgctca cctgctggta
     7861 gggcgcagcc ggcggtgatt tggccagcgc cttgtccgcc ccgtgggcga acgatggaga
     7921 tacccccgcc agggtcatgg ctgctgacgc aagcagaagc gtcgagagtc gcatttcgtt
     7981 gtactccgat catggtccag tgtgacgcgc gctggagcgg cgtcttgtca ttggatgccg
     8041 gttcggagcc aaggttcccg gaatttgtgc gcagccggcc tctcgaggga cgtgcacatc
     8101 gttgactgcg gtcgcaacgc gaaaaacccc ggagcaccag agcctcacgt gctcggtgca
     8161 tccgggacga tcaggttcag agcctgctga ggggcttgga cgtcagccgc ggccgacgaa
     8221 cggcattttc gtcgccatga tggtcatgaa ctgcacgttc gcatcgagcg gcaggttggc
     8281 catgaacacg atggcattcg cgacgtgctc gacgtccatg accggctccg gcgcgatggt
     8341 gccattggcc tgcggaacac cgtgcttcat gcgaccgccc atcggcgtgt ccgcattgcc
     8401 gatgtcgatc tgggagcacg caatgtcatg ggcgcgcccg tcgagcgaga tcgacttcgt
     8461 cagacccgtg atggcatgct tcgtcgccgt ataggcgatt gagttcggac gtggcgcgtg
     8521 ggctgatatg gagccgttat tgatgatccg gccgcccttc ggcgtctgcg ctttcataat
     8581 gcggatcgcc tcctgggcgc agaggaacga gcccgtgagg ttcacgtcga ccaccttctg
     8641 ccaagtctcg tacggcagct cctccatcgg cactgccggc gcgcccatgc cggcattgtt
     8701 gaagagcacg tcgaggcggc cgaagttctc cttcgtggcc gcaaacagcg ccttgaccga
     8761 agccggatca gcgacatcgg tgggcacagg cagcatgcgt ccgccacctt ccaccgccat
     8821 cgaagccgtg cgctcgagct cctccttgcg ccggccggcg agcacgacat cgtacccgtt
     8881 gcgctgcagg gccagcgccg cggcacgccc gatcccgctc cccgcgcccg tgaccagggc
     8941 gaccttgcct gtgctgctgc tcatgactga tgcctcctcc taaaggcgaa cctcgcttcc
     9001 ggcggccatc ctaacggctc gcgcgaaatt gcctagagct tccatagctc tatcgcacat
     9061 ctgcgccgct ggccggccat tgatgctcgc gacgagtgaa atggccaaaa tgcacgcctg
     9121 tcgctcaatt tgacgaaagg cggctccttt gcccttggcc caatctgaac gatgcgctaa
     9181 ccggatcggt gtatgtctga ataccggggg cacgattcta tcgaaggaga attgtatggc
     9241 gttctcgaaa acgctgcttg tcgccgcggg tattgccggc tgcctgttcg ccggatcggc
     9301 gtgggccggg ggttcacgct gggatcggac ggcctactac gaaggcgggc cgatcgaggg
     9361 tcatgtggtc gccgaaagcc ggttcggaca cggaacggtc tccggaccag tgcgccgcag
     9421 ccggacgggt tacgaggtgc ggatgccggg cggcacctgg atcgcctgcc gccggagctg
     9481 ctccgagacg ctccgggtcg agacggtcga tttctgggag aaccgcggcg ccggtcgcaa
     9541 tgcgatcgat tccgagtgcg gtctgctcgg ctgcctcagg tgggagtggt ggttctaggc
     9601 ccctcgctaa tgcctacgta atgaacgggt tcgatgcgcg ctccgctccg atggtgctcg
     9661 tcggcccgtg cccgcagatg aaggccacat cgtcgccgag cggcagcagc ttggtcttga
     9721 tcgcattgat cagcgcctcg tggtcgccgt aggggaagtc cgttcggcca atggagccac
     9781 ggaacaggac gtcgccgacc aacgcgaacc gttgcccgga gttgtagagc acgaccgatc
     9841 ctggggagtg gccggggcag tgcagcaccg agaagctgtg gccggcgacc tcgaccgtat
     9901 cgccttcctg cagccagcga tcgggaacga ccgaccgcgc agccatgccg taagcgcgcc
     9961 cctgctctgc gagcccgtcc agcaggaacc tgtcggcctg gtggggaccc tctatcggga
    10021 cgcccagctc ttcgtgcagc tccgccgcgc caccggcatg atcgatgtgg ccgtgcgtca
    10081 gcaagatctt ttgagccttg ataccgacct cagagatggc gctacggatg cggtcgagat
    10141 cgcctccggg atcgaccacc gccccaacct tggtcgcctc gtcccagatg agcgtgcagt
    10201 tctgctggaa gggcgttacg ggcacgatcg cggctttgag gccggaccgt tgcgcttgcg
    10261 cagttgaatt tgattccatg gcctgcccag tgtcatccga ttgatctggc agactggata
    10321 gagcaggcgg cgcctcatca ccaccccctc gaaacagatc aggggccctg cggccccgat
    10381 ccgatacatc ctgtccgaaa aaaggccgct caggcggccg cgtgctcgtc gttgggatcg
    10441 cgcagcacgt agccgcggcc ccagaccgtc tcgatatagt gccggccacc cgtcgctgcc
    10501 tggagcttct tgcgcagctt gcaaatgaag acgtcgataa tcttcagctc cggctcatcc
    10561 atcccgccgt agaggtggtt gaggaacatc tccttggtga gcgtcgtccc cttccgcagg
    10621 gacaacagct ccagcatctg gtattccttg cctgtcagat gaacgcgttg cccgttcacc
    10681 tcgacggttt tggtatcgag attgacccgc acctcgcccg tctcgatgac cgattgcgcg
    10741 tgtcctttcg accggcggac gatggcgtga atgcgggcga ccagctcgtc cttgtggaac
    10801 ggcttcgtca tgtagtcgtc tgcaccgaag cccagcccgc gaaccttgtc gtcgatccct
    10861 gccaatcccg agaggatcaa aatcggcgtg gcgaccttgc tgacccgcag tgtcttgagg
    10921 acatcgtaac cgctgatgtc gggcaagttg aggtccaata gaatgatgtc atagtcgtag
    10981 agcttgccga ggtccgcacc gtcctcgcca agatcagtgg tgtagacgtt gaagccttcc
    11041 gactggagca ttaactcgat gctctgcgcc gtggcgcgat cgtcttctat cagaaggact
    11101 ctcattctat tcctctcttc gagggctggg ttgccccagg cccgctgccc ctttctgaag
    11161 agcacgcagc tgaaccggcc cttaatactc gtccgaaact tacaccggag aagttaacaa
    11221 agcctaatca cctaactgta ctgtgtgaga cttgatgaaa caacttgccg ccgcggacga
    11281 cttcggatcc cgatctgaaa tcggcaacaa cgcttctgtt acggacaccg tgaatggtgt
    11341 ccgcctggag catcgcggat ttcgtcagtc cccgaaggtg agaacgcacc ccactgcgca
    11401 catccgatcc cgtcccccaa aacggaagcg gtcacgcgca gggtccctgc gaatcaaaga
    11461 attatcagaa ttcgcgggcg caccctagag tcggatggct catttatggc cttgacaggc
    11521 ctgcagaagt tgtcagcgtt ctgtcgtgac tttaccgcaa cttaagcggt ggtctgtacg
    11581 ttaaggaata taatcccctt gatcggtcgt gggatgggtc gactggaacg gagctgatca
    11641 aacaggggaa gcggcgtgag ccgcaggcgt cgcctggcac ggattttgat ttgttggatc
    11701 ttgtcaggct ttgttcgaac atgtcagggg tatcgaacat gaagtcacgg gagattagct
    11761 tccgcctcaa acgctttgcc gctgaggaga agcgccggaa agttgccgat ctcgagcata
    11821 tgatccgcga gttcgagcac atggccatcg agctcgatcg ccaggtgcaa tcggaggagg
    11881 agcggaccgg aatcagggat aagacgcact tcgcctactc cacgctcgcg aaatctgctg
    11941 cgcagcgccg ggacaacctg cgtgcgtcca ttgccgatct gcagacgaag ctcgaaatcg
    12001 ccgtgcgcga gcgcgacgat gcatgtgccg atctcgacaa gttggcggcg ccggagaccc
    12061 agcgtgatct cgatcgcctc ccccgtcgcc cggggcgaga tcccaatatg acgatcagct
    12121 gacggcctcg gcgagggccg atcgaacacg acaattcgcg gagccagtct aatggctccg
    12181 cgggaccctg gctgctttgg gctcattcgg cgcggccgac ccaacaggac accgggcgag
    12241 atgatccgga gcagcacggc gttccgacgc tagcggagcg ctttggcctg acggtgtcag
    12301 cgtgagcaat cttggagatc tatcttaggg cgagctcccg gatcgcccgg gcagcccctc
    12361 gcgctcagac agacgacccg ttcggggcgc gatccggcgc ccttgcttca cgacggatca
    12421 aaagcgaaat cttcaaaggt agcagagctg gcttcgggct gggcatgaca gcccgaaacc
    12481 agaccgggac tggatcagtc gcggtagtcc tgaatgcgtg ttgcgcgcag gcccggtaat
    12541 ccgtgcttgt cgatggaccg ctgccagctc aggaactcat cgaccgtgag agtatatctc
    12601 tcgcaggcct cttccaggct aagcagtccg ccgcgcactg cggcaaccac ttccgctttt
    12661 cgccggatga cccagcgctt tgtgtcgcgg gggggaagat ccgcaatggt caacggactg
    12721 ccgtcgggcc cgatgacata tcgcacgcgc gatctaaact gctgctcagc catggcactc
    12781 tacgcaatac tcagttactc gccaccatgg tggccgaccg accttaattt ggctctaagt
    12841 cgttaggtaa acatatcact cagaactgta ccatgcatcg ggcggaggcc gttaaccttt
    12901 acgttgactc tacattttcg acggaagaga cctctccccg tccctggcag cgacgagttg
    12961 catagcgctt gtgcatcaag gttacttgcc tcggcgggcc aacggcgcca tatgttgccc
    13021 cgggcttccg atacactcgt gtacctcagc ccgggcgccg cctgtgagag cgaccgaacc
    13081 atatttggag ccattctaag ttagacatga gcgcgggaga gacgacagcg ccatcgtacg
    13141 gcgggcccac gacgcgtggc gcgcggcgtc gagtcatcgt ggccatgtct ggcggtgtcg
    13201 actcatcggt cgtcgcggcc ctgctcaagc gagagggcca cgatgtcatc ggcattacgc
    13261 tccagctcta cgatcacggc gtcgcgacgg ctcgcaaggg aagctgctgt gccggccagg
    13321 atattcatga tgcgcgccgc gtcgccgcga cgctcggcat cccccattat gtcctcgatt
    13381 acgagcgtcg gttcgagcgc tctgtgattt cctccttcgc cgacagctat ctcgccggcg
    13441 agacgccgat cccgtgcgtg acctgtaatc agcagatcaa gttccgcgac cttctggaga
    13501 ctgcgacgga gcttggcgcc gatgttctgg cgaccggaca ttatatacgc cgttgcgatg
    13561 gccccgacgg accggagctt cggcgcgcgg tcgacacgga ccgggaccag agctatttcc
    13621 tgttcgccac gacgcgtgag cagctgtcgc ggctgtggtt tccgctgggc gggctgagga
    13681 agccgcaggt gcgggagctc gccagcgagc tcggcctgtc cgtcgcctcg aagccggaca
    13741 gccaggacat ctgcttcgtt ccctccgggc gctatgccga tgtgatcgag cgcctgcgcc
    13801 ccggcgcctc cgagccgggc gacatcgttc acgtggacgg ccgtgtcctt ggccggcacg
    13861 acggtatcat gcactatacg atcggccagc ggcgcggcct caagctggcg ggcggaactc
    13921 cactttacgt cgtgcggctc gaccccgagc gcaggcaagt gatcgttggt ccccgtgaag
    13981 cacttcggac cgatgtcctc gagctccgga acgtgaactg gctcgggtct cgcgccgaag
    14041 cggcggcagc ccgcgacggc ctgccgatcc gggcgcggat ccgttcgaca catacgccgg
    14101 tagaggcgac gctcgtcctc gatgaggcac tgggtagggc acgggtgctg ctccccgaag
    14161 gtgagcacgg cgtggcaccc ggccaggcct gtgtcttcta cgacgatgcg gggcctgagg
    14221 cgcgagttct cggtgggggg tggatcactc ggaccaagcg agcgatcgat gaggatgctt
    14281 cgcggacgat gccgcgatcc gttgcagcgg gtctggcaac ctgacgcgag ctcgagagta
    14341 atcattgaaa ggtgggagag cacaggtgtt tgacggtaga gttcgaatgg ctaagcaaag
    14401 ccgtcgcccc ttcggcggga cactcgcggc gaaagccctg ccgaacatgg acgagtcctc
    14461 ggtcatcagc gcctaccgcc gctgggctcc catctacgac cacacgttcg gcaagatcgc
    14521 cgctgagggc cggaagcacg ccgtcgaaat catcaacagt cgtaccggaa gggtgctcga
    14581 ggtgggcgtc ggcaccggcc tctcgcttcc gagctatggc aagcacctgg agatcgtcgg
    14641 tatcgatctg tctccggaca tgctggagaa ggcgcggcag cgggtcgcca acgaaggtct
    14701 cgacaacgtt gccggcctct atgagatgga cgcgggtcac ctcacgtttt cggacaattc
    14761 gttcgacact gtggtcgcca tgtacgtgat gaccgtcgtg cccgagccgg agcgcgtgat
    14821 gcgcgagctc gcccgggtct gcaagcccgg cggcgaggtg atcctcgtca atcacttcag
    14881 ccaggaagaa ggtgtccggg gctgggtcga gcgccgcatg gcgccttttg ccgaccagct
    14941 cggctggcgt ccggtgttcg atgtcgaccg cgtgctcgtc tgcgacgatc tcgaagtcgt
    15001 ggagcgccgc gcgctcaggc ccatgggcat cttcacgatg ctccgcatgc gcaagcgtca
    15061 actggcctga tcctggccag ccgcactgcc cagtcgcggc gctggctttc ggcgacggat
    15121 cggagcctgc caaggtatgg cgctcgcttg acagactgca gccgcgtttc ttatatcgcg
    15181 gcgcagaatg gcgggatagc tcaggtggtt agagcgaagg tctcataatc cttaggtcgg
    15241 cggttcgagt ccgcctcccg ctaccattcc gagcctacgt ccctgatatt aaagagaaaa
    15301 tccgaatatc ggcagcagca caggccgcgc cgagatgcct tgcacggctc ctgtctcgct
    15361 gaccctcaga acgccacggc tgctgacgtg ggtttgcgag acaagcccgc tcggccgctt
    15421 gaacggcgtg cttccgccgc gggatccagc cctggctgac gtagggcgct tgacatctcc
    15481 cggtatggcc tgtcgcagcg gggcatctgc gcgcggctgg agccgcccat gacgctccac
    15541 cagcgaaagc ttctcttatg gcgagtgctt cctgcgcggc cctcaccact agacagacgc
    15601 accgtatctt catatattcg caaatatgaa acttaagacg aagatcgacc ccaatgccat
    15661 gcgttcagcg gcggacgaag ccagcgcatt gctgaaagcg atgtccaacc gccatcgcat
    15721 gctcatcctc tgccagctca tcgatggcga aaaatccgtc ggtcagctcg ccgagttcct
    15781 cgggattcgc gatgcgacgg cgtcccagca cctcgccctg ctgcggcgcg accgcatcat
    15841 ttctgcgcgg cgggatggtc agacgatctg gtaccggatc tcgagccagc ctgcgctcga
    15901 ggtcatgcgg gtgctctacg acatctattg cgcgcaatca aaggcacaac caacagcgcg
    15961 gcaatgatag gcagcagtcg aactgaagga ttgagcttgg cttggaggga tgttcgcatc
    16021 ctcccgctcg cacgagtgct cactgatgct ccatgcccta gcaccccgtt tcgatggttt
    16081 gcgccgcgac cgcgaaattg agcccctggt caccggcgtg caattcggga atcgttgaag
    16141 gagacaaatc gatgacaatc gatcgcgccg tcatgatatt cgccggcttc atgatcctcc
    16201 tttctctgac gctcggctat tacgtctcgc cgtactggta tcttctgacg gccttcgttg
    16261 ggctgaatct catccaggca tcgctgaccg gcttctgtcc cgccgcaatg gtcttcaaga
    16321 aactcggctg caaaagcgga aacattttcc gttaatatgg cccgcccgac gcgaatgcag
    16381 gcggcgtctc cagcgccgtt tgccaggggg agatgatcat gttctcgcgc gccattttcc
    16441 tcgtcctgtc cacattccta gggatcgctc ctgcggcggc cgatttcgtc gtcaagtcca
    16501 tttcgatccc ggacatgaag gcggtgttcg gacaggtcga aagccgcaga atcgtaccgg
    16561 cgcgggcgcg catcggcggc tcgatccgtg cgatcaaggt cagtgagggc aacgaggtca
    16621 aggaaggcga gaccattgcc atcgtcgtcg acgacaagct cgcactcgaa atgagcgccg
    16681 cgaatgccaa gatcgaggcg ctcaattcac agttggagaa tgcgcgcatc gagctcgacc
    16741 gcgcacagca gcttctggcg cgcgggaccg cgacgcaggc gcgggtggat caggcgaaaa
    16801 tgcagttcga tgtcgttgcc aatcagctgg cggccgccca agctgagaaa tcggtcatcg
    16861 agcagcgcgc ccgcgagggc gaggtgctgg cgcccgcgga tggccgggtg ctgacggtgc
    16921 ctgtcacgct cgggtcggtg gtgcttccgg gtgaagaagt cgcgcgtatc gccagcggcc
    16981 gatattatct gcgccttttt cttcccgaac ggcacgctgc gggaatcagg caaaacgcga
    17041 ccgtcctgat cggccagcgc ggcttgtcgc ccgcaacaag cggcagcgcc tcggaggcgc
    17101 gccccggccg catcgtcaag gtctatccag aaatttcggc cggccgggtc gtggccgacg
    17161 tcgaggtgga cgggatcggc gactatttcg tcaacgaacg cgccctggtc tggattccgg
    17221 tcgcggagcg ctcagcgttc gcggtccctc cagaggcggt cagcacgcgt cacgggatcg
    17281 attatgtccg gatcgccacc gaaacaggat cgcgcgaggt cgcagtgatt ttgggcgaga
    17341 ccattttcac tgccgacggc ccacgcgtcg aggtgctgac gggtctgcat gacggggatc
    17401 gcatcatcat ggcggatcgc aagccatgaa gctcggactt gccgggctcc tcacgcgcgg
    17461 ctccatcgcc tcgccgctga caccgctttt cctgctggca gctttcgccc tgggcctcgt
    17521 tgccctcgtc accctgccgc gcgaggagga gccgcagatc tccgtgccga tggtggatat
    17581 ccacgtcgcc gccgacgggc tcaaggccga ggatgccgtc aagctcgtca ccgagccgct
    17641 ggagaccatc atcaagagca tcgacggcgt cgagcacgtc tattcgcaaa ccgaggacga
    17701 cggcgcggtg gtgaccgcgc gcttcctggt cggcaccgat gccgacgccg cgatcctgcg
    17761 cgtgcacgag aaaatccgcg ccaacatgga ccgtatcccg ttcggcatac cggagccgct
    17821 catcgtcggc cgtggtatcg acgacgtcgc catcgtcgtc gtcacgctga caccaactcc
    17881 ggctgcggca gaccgctgga ccgccaatga tctcacccgc ctcgcccgcg agcttcaggt
    17941 cgaggtgctg aagctgcccg atatcggcct cacctatatc gtcggcgagc agccggagga
    18001 gatccgtatc cagccggacc cagagcggct gtcactctac ggcattacgc ttcagcagct
    18061 cgcctccaaa atcgcaggcg ccaatcgctc cttccagtcc gggcgggtcc gcgaagccgg
    18121 cgagcagcgc gcgctagttg ctgggcagac gctgcagaca ccctccgagg tcggcaattt
    18181 gctgctgaca gcccgcgata gccggcccgt ctacgtgcgc gatgtcgcca ccgtgatatt
    18241 ggcgagcgaa ccgaacgaga cccgggtcac gagcgtgcgc aaggcccccg gcggcctcga
    18301 gcggacgcct gcggtatcgc tcgccatcgc caagcggccc ggcaccaatg cggtcgtcat
    18361 cgcggacgcc atcgtggagc gcctcgagca ggcgcgcggc caaatctttc cggcggacgt
    18421 cgaaatgacc gtcacccgca actacggcga gacggcgaac gagaaggcga acgagcttct
    18481 tttccatctc ggcctcgcga cggtgtcgat cgtgatcctg gtcggcttcg ccatcggctg
    18541 gcgcgaggcg ctggtggtcg cggtggtgat accaacaaca attctgctca ccttgttcgc
    18601 cgcgcgcatc atgggctaca cgctcaaccg ggtgagcctt tttgcgctga tcttctcgat
    18661 cggcattctg gtcgacgacg ccatcgtcgt catcgagaac atcgcccgcc attgggcgat
    18721 gaaggacggg cggccacgcg tcaaggccgc tgtcgaagcg gttgccgagg tcggcaatcc
    18781 gaccattgtc gcaaccctga cagtcgtcgc agcccttctg ccgatgctgt tcgtctccgg
    18841 catgatgggg ccctacatga gcccgatccc gaccaatgcc tcggcggcga tgctgttctc
    18901 cttcttcgtc gccgtcatgg tggccccgtg gctgatgatg aagttcggcg gccacgcaca
    18961 tgctcagtcg catgaccacc atgccgcgca gggcggatgg ctgggccgcg cgtatgtcaa
    19021 agttgccgag cccgtcctgc gcacgaaacg gcgctcatgg gcctttctgc ttgcagtcgg
    19081 cgccgccaca ctggcgtccc tcggcctgtt ctacacccag cacgtcacgg tgaagctttt
    19141 gccgtttgac aacaagaccg atctccaggt ggtgctcgac ctgcccaagg gcgcctcgat
    19201 cgaggatacc gaccgagcgc ttcaggccat ggtcgacgag ctggccggcg tgcccgaggt
    19261 cgcttcgttt cagacctatg ccggcaccgc cgcaccgttc aatttcaacg gactggtgcg
    19321 ccactattat ctccgctcgc agccgcacca gggcgacatc gctgtcaatc tcgttgccaa
    19381 aggcgagcgg acccgcacga gccatgatat cgcgctcgag ctgcgcgagc ggctgaaagc
    19441 ggtcaagctg ccggaaggca gcgtgctgaa ggtcgtcgag ccgccacctg gcccgccggt
    19501 gctgggcacc ctgcttgcgg aaatctacgg cccggatcca gacacgcggc gcgcggtcgc
    19561 gaccaaggtg cgcgaagcgt tcgccagcgt acccttcatc gtcgacgtcg aggacagctt
    19621 ccgtaatcgc ccggagcgcg ttcgcatcgc catcgaccag gacaatctcg aatattaccg
    19681 cgtcgagcag gctgacgtgt acgatacgat ccgcacgctg gccggcggga cgaccgtcgg
    19741 ctattcgcac cggggcggcg ggcgctcgcc gatcccgatc cgtatcgcat tgtcgaagag
    19801 cgagcgcgtc gtgggtgagc gcactctggc aaccccagtg ccggccaacg cattgccagg
    19861 cgaccgctcg atcgtcgagc ttggcgacgt cgtctcagtg acgcgagaga aagcctccta
    19921 tccgattttc cggcacaatg gccgtgccgc ggaaatggtg atggccgagc ttgccggcgc
    19981 gttcgaggcg ccgatctacg gcatgcttgc ggtgagcgat gccatagccc aggcagactg
    20041 gggtgaggtg ccgaagccca ccatcgcgct gcatggccag cccaacgatg aatcgcaccc
    20101 taccctgctc tgggacggcg aatgggaagt cacctgggtc acgttccgcg acatgggtgc
    20161 cgcgttcatg gtggcgatcc tcggcatcta cattctcgtc gtcgcgcagt tcggctcgtt
    20221 caagctgccg ctcgtgatcc tcacgccgat cccgctgacc ttcatcggca tcatgctcgg
    20281 gcattggctg ttcggcgcgc cattcaccgc gacctcgatg atcggattca tcgcgcttgc
    20341 cgggatcatc gtgcgcaatt ccattctgct ggtggacttc atccgccatg cgcgcagccc
    20401 agagcgaccg ctcatcgacg tgctcctgaa ggccggcgcg atccgcttca aaccgatcct
    20461 gctcaccgcg ctcgccgcca tgatcggcgc tgccgtgatc cttgccgatc cgattttcca
    20521 ggggctcgcc atctcgctgc ttttcgggct catgtcctcg acggcgctga ccgtgctcgt
    20581 catcccggca atttacatcg tcctgaggag caacggatcg aagggaaatg ctgcgcgtgg
    20641 cgaaaggacc ggttcatcgc agcaaggcga accggaaatc attcccactt agacggtttg
    20701 gaaacgaaga gggctgcgcc agttctgaat tcagatttcg gccgccatcg agcggctgtg
    20761 agacggacat cctggagaca gcaaatgcaa accgattggc aggcattcac acgtgagctc
    20821 tctggcgggc tgcgcgactt gcggggcggc gccccggagg taatgaaagg gttctcagaa
    20881 atcgcccaag cagctttgaa ggccaatgcc ctcgacacca agaccaagga gctcatcgcc
    20941 cttgccatca gcgtcgccat ccggtgcgac gattgcatcg gcttccacgc caaggccgcg
    21001 gtcggccagg gcgcaagccg cgaagaggtc ctggagacgc tggggatggc gatctacatg
    21061 ggcgccggcc ccgccgtcat gtatgccagc cacgcgcttc aagcattcga tcagtttgag
    21121 aaagctcgac cagcgaggtg agagcccaaa cgagctccag acctccacgg cagcacctgg
    21181 gagccgcttg ccgcagtcgt tccgggcgat gtcatccttg ccttgtgatc tttttcgctg
    21241 ccccgcctgc cttctcgatc tcactccctt ggcgcggcta gcggaggttc tatgaagtgg
    21301 cgctcccagc gagcatccat cggtatccac tgatgctcgg tgttgcaata agggcaatgc
    21361 gccgtcattg gcgtacgagg aagcgatcga aagcttgttt cgctcaggaa tatccccggt
    21421 ctgtattcct ttcccgtgac ggggcagcgc aacacgacct ccccgaaatc tgcctccatc
    21481 ccagaatcga actggctcaa ctgccgctac tcccaatcta catgccaccg ctttcggatc
    21541 gctttgtccg aaactttcga cttgagcgat gtcgtcccag cttgggagca gactattctg
    21601 gatctacccg cgctcaagct ctagcagatg cgcgcccaaa tcggtttgcc ttttcaggca
    21661 aacgcaccga acggcgatat gatcccgcaa ccatggccgg ctgtacggct ggcgcgtgag
    21721 cgctcgctgt ccgctccccg gcggctccgt ggaatgcggc gcagctccaa atgcaaccgt
    21781 cccggcacga ggcaagccgt ttacgcgacg ctgcccttgc gcagggagcg caggaaggat
    21841 accgcgatgg gcgatagcaa gaggatgaac gccagcgtca tcaaccctgc gacgagcggg
    21901 ttcgaccaga agatgctgag cgaccccttc gacatgatca gcgattgccg gaacgtgtct
    21961 tccgccttgt cgccgatcac catggcgagg atgagcggtg cgatcggata gtcgagcttc
    22021 ttgaaggcgt agccgagaac gccgaacacc agaacgagcc agagatcgaa gcccttgctg
    22081 ccgaccgtat aggcaccgac cagacagacg acgacgatcg ctgggccaaa aatgctgaaa
    22141 ggaatgcgca ggatcgcggc gaagagcggc actgtcgaca ggacgaggat gaccgcgacc
    22201 acattgccga gatacatgct ggcgatgagg ccccagacga aatccggccg ctcgatgaac
    22261 agcatcggcc cggggttgag gccccagatc atcagaccgc cgaggaggac agcggccgtc
    22321 gccgagcctg gcacgccgag ggccagcatc ggcagcatgg cgcatgtgcc ggcggcatgg
    22381 tcagcggtct cgggcgaaat gatgccttcc ggttcgcctt tgccgaactt gtcccggtcg
    22441 cgcgagaagc gcttcgcgag gccgtagctc atgaaggacg ctgccgtcgg gccgcctggc
    22501 gtgatgccca tccagcagcc cacgagcccg ctgcgcaaga gcgccagcca gtggcgcggc
    22561 atgctgagcg cggtgttgag gacgtcacgg ggcgaaatgc gcgccctgag gccctgcagg
    22621 cgcaagccgt cctcgatggt gatgacgagc tcgccgatcc cgaacagccc gatgacggcg
    22681 accaggaagc tgattccggc aacgagttgg tcgaacccgt aggtgagacg caggttgccg
    22741 gacacggaat ccatgccgac ggcggccagc gtgaacccgg taccgatcgc gacgatcgtc
    22801 ttgaggggag aagcgccgcc gaacccgacg aagctcgcga aggcgagaaa ataaacggcg
    22861 aagtattcag cagggctgaa ttgcatcgcg aagcgcgcga cccacccgga aagcagcgtg
    22921 atgacgatga caccaagcag cgcgccgaag cccgccgaca ggaacgcata gctcagcgcc
    22981 tgcgtcgccc gcccctgctt ggccatcgga tatccatcga atgtcgtggc gaccgatgat
    23041 ggctctccag ggatgttgaa caggatcgag gtggtggagc cgccgaacag agccccccag
    23101 tacatgctcg tcagcagaat gatggccgcc acggggtcca tcgtgaaggt gagcggcagc
    23161 aacagggata cgccgttggg tgcgccgagg cccgggagca cgcccaccag gataccgaga
    23221 agcacaccta ttatcattag aatgatgtgg tgcgcggtga tcgcgacgct gaaaccgtgg
    23281 aatagtgcct ccagcccctc catcgccctc tcctagagtc ccagagcgtc ttcgagcggc
    23341 cctttgagga ggggcgtctg gaagccgatt tcgagaataa ggtacaggga gacagccatg
    23401 ccaatggcga cgagcgccga aaaaccggcg cgatagccgc cctggaattt catgctggcg
    23461 aaaagataaa gcgccatccc gacataaagc cccacccaga gggagacggc gacaagggcg
    23521 acgatgggaa gggagaagaa gataaccgag cgcagttgcg cggatgtcag aaaactctcg
    23581 tcgcggtacg cccgctggat cagcgtctga aaaataatgc cgacaccggc aaccgtgacg
    23641 atgaggccga cgtaaaaggg aaatgtgcct ggctctgggc cggacgggct ccagcccacg
    23701 ccaaattcca aggcaccgag ggtgatgatg aggccaaaca ccgcgacgat gatggccatc
    23761 accaattcgg ctccgaagcg cgaaatcatc cgcaaccgct ccaggagaga aggcggggcc
    23821 gaagccccgc agaattgaac ccgcgaggaa ggctggtcat ttgaccagcc aaccctcctt
    23881 ctcgaacacc tgcttgttct tggcttcgtc gtcggcaatg aacttggcga gctcgtcgcc
    23941 ggacatgaag cgtgcggtct gcaccgtgcg ggtgacgtac tctttccact ctggtgtctc
    24001 gctcaccttc ttgaaaagct ccgtgtagaa ggcgaccgcg tcggcaggca cgttggccgg
    24061 cagccagatc gtgcgcggca tctggaagct atcgatcgga atgccttcat ccttgcagag
    24121 cgggatgtcc gaccagccca tgtcgcccgt gaccttcggg ccctcgggca gacgggtcgg
    24181 gctaaacacg cacagcggac ggacctggcc tgctttccac tggccaatgt tctcgttcgg
    24241 gttgttcgta ttggaatcga tatggccgcc ggcgagctgt accgctgcga ggccgccgcc
    24301 gttgaagggc acgtagatga atttcgcgcc agtcgcggat tcgataaggc tcaccagcgt
    24361 ctggtccgta tccttcgact ggctgccgcc catcttcaga gtttcgggtt tggccttggc
    24421 ggcggcgatg tagtctttgg ccgtcttata ttccgagccg gcattgaccc acagcaggaa
    24481 ttcatcgagc gccatggcgg cgacgggcgt cagatccgac atcttgaacg ccacgttggc
    24541 gacgtaaggc agaagatagg cgttgttggt gccgaagatc accttatgtg gatccccggc
    24601 cgcaccgcgc gcataaatat agccttcagc tccgctgccg ccgcccttgt tggtgacgat
    24661 gatcgcttca tcgaggagct tgttcttggt gatggccgac tgaacgacgc gagcgaagtt
    24721 gtccgtgccg ccgccggccc cggcagtcac gacgaactcg atcgatttcg tcggctccca
    24781 ggcgtgagcc gccggaacac cgagcatcga tactgcggca gcaataccga tcaattggcg
    24841 aacgtggagc atctcccatt tcctcttcag gttgatcatt gttgcagctg ttggatcagc
    24901 cgtcgcttgg acttgggctc aagatcttca cgcagctttt gcatccggac ggtgtcggtt
    24961 ccccgccatc tcgatggcta ggagcagcgc cgtccggctg gcgccgaggt cggcgatccc
    25021 cttgccggcg atgtcgtagg ccgtgccgtg cgccggcgtg cagatgggga acggtaatcc
    25081 gccgatcagg gtgacgcccc ggtcgaagcc catgagcttc atcgcgatct gcccctgatc
    25141 gtgatacatg gtgagaacgg cgtcgaagtc gccctttctt gcgcgcagaa acaccgtgtc
    25201 tgatggataa gggcccttgg cgtcgatgcc catctgctgc gcttttgtaa ctgccggtcc
    25261 gatgatgtcg atttcctcgc ggccgaagat gccgccgtcg cccgcatgcg gattgagccc
    25321 tgcgacggca atgcgcggat gcgtgaagcc ggctgcacgc agggacgagt gggtcagttc
    25381 gagtgagccg aggatgcgct cttgcgttat ggcgcttgcc accttgctca acggaatatg
    25441 cgacgtgacg cgcgcattcc agagtttgcc caggatgttg aactcgctcg ccggcccatc
    25501 gtagccggtc acgtcgcgca cgaagcgaat ttcgtcgtcg tagccgggat aggcgagccg
    25561 catggccgcc ttattgaagg gcgtgaagaa caccgcgccc gcctttccgg cagccgcgag
    25621 ctgcagggca cggcggaaat tctcgctggc gaatttgccg cctgcgaccg tcgccgtgcg
    25681 cggggtgaca tcggcggggt cgagactgcg aaggtcgacg aaaatcggcc gggtcgcatc
    25741 ggcaggaatc gcgtcctcag ccgtcacgac gtgcacgtcc gggtcgacgc ctgcgaccgc
    25801 cgctcccatc tcgagaatgc gcttgtcccc gaaaacgacg agcctggcct tcgcccggat
    25861 ctccgggtcc gcgatcaggc gcgcgctcag ctcaggactg atcccggcgg gatcaccgat
    25921 tgcgaatgcg atggtgggct gaacatcgcc gctgccggat tgcatttggc cccttcctgg
    25981 cggttccagt gttgctgtcc cggcgagtat atgggggccg tcttgcgatt caagtatttt
    26041 gtatgcagaa tgcaacgagg ctcactgaac aacaggcgcg cagcaggggg tctttgaagc
    26101 cgccgcagga ggttggcatg agcgaggacc cgtcgggtga catccggccg tcggcggccg
    26161 acggcatgga aggcatgctg ccgatcgcgc gccggacgct gcatgacgag gtcgtggagc
    26221 gtttacgcga tctcatcatt caaggtcggc ttgcgcccgg cacacggatc aacgaggtcc
    26281 aggtgggggc gctgctcggc gtctcgcgca ccccgctgcg cgaagccatc aagacgctgg
    26341 cacgggaggg gctggtcgag aacgtgccct cacggggcgc cgtcgtgcgg cgcttcagcg
    26401 aggccgacgt cgcacagatc cacgaagtgc tgaagacttt ggagcagctt gcggcgcggc
    26461 tcgcttgtga gcgtgcggac gatgagacaa tcacccacat tcaccggctg caccagcgca
    26521 tgctcgaatt ctatcgcaac ggaaaccggc tcgaatactt caagctcaat caggccattc
    26581 ataccgcgat cgttcgggct tccgggaatg ctgcgctcgc cgaaacgcat gagctgctgc
    26641 aagccaggat caggcgggtc cgctacacgg gaaatgacac gccatcgcag tgggcaggcg
    26701 cggtcgcgga gcacgaggaa atgatcgagg cgctcatcgc gcgcgatggc gagcgcctgg
    26761 ccaatgtcat ggcccgccat ctcgatgaga cgatgagccg ggtgcgcgat gcgttatgaa
    26821 gcatcggcgt gatatcaggt ttgcaatcct gcgtcattat ccggcgccgc tgtcatctgg
    26881 gcgacgctct cgccggctct cttgctcagt ttggcggaca agccaggccc gggcatcttc
    26941 aatggcttct ctcgtcgact cgccgatgat cggagtgagc tcatagcccc ttattggatc
    27001 ggacagacga agcctgcctt cctcgacgag ctgttcgaac gtcctccgat ggaggccggt
    27061 cacgtaaagc cggccgtttc gcgcgttcaa ctcagccgca tatctggaaa gcacgtgggc
    27121 gagagtggcg ccgacattcg gctggcctct caggcgaagg atgacgaccg gattttgggc
    27181 gccgttcggt ctgggaagct gccgactgag aattgcggcc cccgcataaa acaaatgccc
    27241 atacacatag agcaccgtca cggcgttgct tggaaggcgc ttggccggct tccgctcagc
    27301 gatctccccg tcgggtcgca aatactgctc gacgagcaga acatctatgg aagagcggta
    27361 tatgtagatc agggctgaaa gcacggcccc gaatcccact gcggcctgca gcggcaggaa
    27421 cagtgtggcg atgaaggtcg aggcagtcac gagccgcggc ggcccgccga cctgccagat
    27481 caacctgatg tccattggtc taatcgcctt aatcccggcg aagatgagaa tgccagcgag
    27541 ggagggcagg gcgatatagg atatcgggcc cgccaatatc accacgagag ctgccatcca
    27601 gactcccgca aatatgactg accagcgcgt ccgcgccccg gacgccacgt tgatggcagt
    27661 cgcccctaga gaaccgccca ctggtattcc atgcaaaaat ccggatgcca cgttggcggt
    27721 gccttgaaca acgaaatcgc gcgacgctcg gctccgagat ccgtccgggt tcggcacgct
    27781 ctggctgatc ccagagctct gcaccaatgc aaccgcggcg atcgcgagtg ctccggtgac
    27841 aagctcgaat gtaacgtcga acggtgacgg tacctcgaag tccggaaagc cgttgccgat
    27901 ctcgccgatg ttccggacga gctttacgtc caagccgaaa agataagtga caccggacgg
    27961 aacgagaatg gcgatgaacg taccaatgct gccgagacca atattcggga gaacgagcgc
    28021 cagaaacaat gtcagagcgg ccaacccgag cgtcggcaaa tgcacctggt ccaaattggc
    28081 cagcagatcc aacgtttgcg tcaccctgtt ctgaccgatc ggttcatagc cggtcgcgtt
    28141 gggtagctga ctgagcacaa gcagcacgga aatgccggcc agaaatccgg ccatcacgga
    28201 ataggacacg aatcgggtca atcggcccat tcgcagcagg ccgaacgaga tctgtgccag
    28261 gccgaccaga acaaccagca cgacgaggat cacatcgcgc tcctgggcgg acagggcaga
    28321 cagcacctgg cccgccataa gtgagacggc gctggtcgtg gtggtcacca taaggtgggt
    28381 gctggagaaa aggccgccaa cgatcggccc aaggatgcaa gcgtagaggc cgtagaccgg
    28441 attgacgccg acaagcaagc cgttcgccat cccgtcaggt acgctactga tcgcagtatt
    28501 cagcccggca attccgtcat gccaaggtcg agggggccct ggcgcgcgct ttcgagcgca
    28561 gttcgccacg gcttcgcgca gatgtgcgag gcccggctgg tcaagctccg tttccctctc
    28621 gaggaccttc ttcagctttc gtgccatcgg agaccgctat cgagatcgcg tccgttcaga
    28681 actccgcaaa agctggacta gccagaagca gcctgtcact cgagcttgat ccggatggtc
    28741 tgaacgccct cctccacctt gacgtccaac acgcgagtct cgttctcgta aggcacgacg
    28801 ctgatgttgc cattccgctc catataagcc cgccgaatcc tcttcggatc ggtatgaccg
    28861 gattgcagcc ggagcgcctc gttgaggtct ctgggggtca aattcacgct ttgcattccg
    28921 tcttcttgca ccacgccgtc cttgatcagc aaaacaggct cgcctttcac gacggggccg
    28981 aatatggaag tgtgataggc aatgaaagcg aacagccagt gcagtccaac cagcaccaat
    29041 cctgcaacca ttgtggggag gaacagcgcc gacccgttga cggttcgact caggatcgat
    29101 cccagcatga taccgacaat gacgtcaaaa gccgtggcct gacttacaaa gcgcctgctc
    29161 gcaacgcgca ccaacgccag cgacgcgagg tagacgatcg cagtccgacg gccacttcag
    29221 ccgagttcag atcagcgggc tcccggccga ctccaaagaa ccactgaact tgatcccaca
    29281 ctgtcaggag cacgcgcgct cctcggtcgg tagccggcgt cattcgacag cgattgagcc
    29341 ggaacgcttt ttcaccaaat cggtatcgct ctggttgtca gtgtctgtgg cacatcgttt
    29401 cacgtcttgg cctttgcttt cactcgacgg gggcgtgctt tgcgaggttt gctgtatatc
    29461 accttccgcg tgccaaacag atcgagatcg aaaggcaccc gatcttcgcc gatttcgtgc
    29521 tgaatcaggg cattcatctt gtcgagcatc cgttcgacaa taaccgggtc ataaccagga
    29581 tgatccgggt gtccgatgtt ctccatttcg cccggatctt tcacgagatc gtgcagcgta
    29641 acgtcgctgc gggcgtaaag ttcgccgagc gtgaagggaa gatcgtattc gagcgggctg
    29701 aaccaacgca ccatcttgta tcggccatcg acaacggcgc gaaagaatgt gcgattcgtg
    29761 aaatccggag cgccatagag gcgacggact tgctttggat cggccttgcc caaggggcct
    29821 tcatcaagat cggtcagcgc ctggagtgcc cccgacagag cccagtttac atcgagtgaa
    29881 tggagcgcgt cccagcacac gagagcgccg tcgccaggca catcagcact gccccgcggt
    29941 ccatgcgact gcggattcat aatggcactg cgcaaactgc gccccttcag gtgcggatag
    30001 cgggccttaa cctcatccgg ggcgagtccg gcgaactcca ggatcgtcgg cacaagatcg
    30061 agatgcgatc cgacggcaat ggtgcgcttt cctttcggcc cgccgggcac gcagactgtc
    30121 agattcacga ttgtggcttc gtcaaaatgg attgccccct tctgcgtcat gcggtgtgcg
    30181 caattcattt cgccatgatc gcttaagaaa atcacaatcg tatctttcca aagatccagc
    30241 cggtccatgg cagcgaggac tttgctgaat tcagcgtcga cgaggcgcat gcagttgatg
    30301 agatagttgc gccgtttcaa ccaaagatcc gttcgctcat ccggaacgcg accgtaattc
    30361 tggtcgatga actccttgta gtggtgcacg ccgaatggct gcagccgata atcgtcatcg
    30421 aagttgtccg gcatctcgac tggccactcc ttctcgaacc agcccaggcg ctgcacggtc
    30481 gactgcaggc ccttgatcat cccgttttca tcgggcgcct cgatcgggtc cgtttgcagg
    30541 aacatgatgt cgtgcggatt gataaggctg cagatgagca gccagggttg gtcgagctcg
    30601 gcacgcttgt gctccagcca gtcgatggtc tcgaaggtgg cagcaccgtc gagcatggcg
    30661 ccttggagcg gagcgccaaa catttcgccc cattgctgat aatcggaaaa gccgtaacgc
    30721 tcgagcgcat cttcgctctt cggcaccgcg gagacatgcc attttccctt gaaggcggtg
    30781 tagtagccct gctcgcgcag aaggtggccg atcgtcggaa agtcctggct tagttcatcg
    30841 atccaggcaa agttcgtatt atccgatagt cccacttgct ttgcgtgcac gcccgtccac
    30901 attgccgcgc gcgatggcgt gcagaggggc actgcacagt attgccggtc gaagctgacg
    30961 ccctctgcct tcaggcgctg catcgccggg aactccacct ccttgggcgc ccgcatgtgt
    31021 gtgtcccact gatccatgca caggatcaga aagttcgggc gatcattgcg cgtttttctc
    31081 ttggttctgg ccatccaatc ttttctcctc ctgaacgcag gccgtttaac ctcgtcccgt
    31141 cagtcttcga tcgtagagat cccggatatg tgtgtcggcg cgttttctct gcaggcgcca
    31201 agccgtcggg aatagaatgg ctatgccgat caccgaactg acgataactg ttgccagggt
    31261 atcgggatcg tctatgctct gggtcgcaac cacagttgcc gccgagacat tgcgctgacc
    31321 tgtgccgagc gccagaatat tgtggctcgc cgggtcgcgg cctcccagca aaaagccaat
    31381 tgcataagcg cccgctgtca ggatcgtcgc ggccagtatc gcgcctgatc cgaaaaggcc
    31441 aacgacttca cggaagttga cgatgagtgt gaaggagacc atcgcaacga gcgcaatgct
    31501 tcccagtgtg cccatcaaag gccgaaggcg ctgagccgac cgcggcagcc aagctcttgc
    31561 gagcaggccg atgcccagcg gcaggagcat tgccaatacc agcggttttg cgatcgcagc
    31621 ggcatcaacc gtgactgcag gcacgaccag gggcatcatg gtgggcaggt agagaatggt
    31681 gaacggaagc agcagaacga gcagacttgc cgccaaaccg acatcgtgat cggccgcttc
    31741 cgtaagcttg atcagaaaag gtgcccctcc agccatgccg agcagcatga ggccgatcgc
    31801 tatcggctcg tccaatgaca gccaatgcac aatggccaaa gccaagatcg gaaccagcac
    31861 gaaattggcg atcagcgcgc gtgctacttt gcccgcatcg cgaaaaggat cgataatctc
    31921 ctgcacggtg tagccgaaac ccaccgaaag catgctggct acactgaaaa caagaacgga
    31981 tatgtctagc aggccggaca gcaaaacaat tcgaccgtct caagttggtc agcgaaacgt
    32041 gccggcagtt ggggagttcc aaggcctaac agactagccc cagctcggtt ggagccgccc
    32101 caggcaacga gaggcggggc gggctcgacc agatcacggc tccacaaccg ctcgatcgcg
    32161 atcgtcaaaa tcgcagttgc acctccgcgt caataaactt gaccgtattc gatggcccgg
    32221 tctcctcgat gaagcgaccg ggctcgaata cggagaaagc ggctccgatg tcgacaccgc
    32281 gggccggcga ccaggcgagc accacctcgc tttgcgtgcc gatctaacgg gctttgctgt
    32341 cgcccgatgc ccggagcagg ttcccgggcc ccggtagacg ccgtctttca ggctttcgcg
    32401 ccagaagaac acggatgaaa tgcagtcaag cgctgatgag gcgaagcctt cggctaccgt
    32461 accgtcggag taatttagct gccccgctgc tcgcagacac cgtgccgccg cagcacgttc
    32521 tcgatgtcag cgacggtccg gatctccatc atctcgtcca tggagagttg gatggcaaac
    32581 gtctcctcga gacagcgcac caaagcgatg tgctggagag aatcccaccg ggagacgtca
    32641 tccggcgagg tcgacggcgc cagcggcccg ctgtactgga agacgtcctc gaaaatggcc
    32701 gcaagctgcg ggtaggcaat cccattggcc gaagtggacg ggcccattca cgtctcccat
    32761 tgaacgacga cgaagttgga ccccggcagg ccggccttgc cgatcgtcca actccaagtg
    32821 gtttgactgg gcccggtcga tacctcccga aagcccagcg aagagtagag gtcgcgaacc
    32881 tggccgttct tggccgatgg aacgtaggtg cccgtgacct cgcgcacgcc gcgccggccg
    32941 agctcttcca gcagggcccc catgaaggcg cgctcgattt cgcggccgat cacgcggcag
    33001 ctcatcaaga aggtgaggat ttccgcggcg gccccatcga tgcgcgcggt cgcggcgatg
    33061 acgatgccgt gattaccgaa acggtcgctg gcccgcccct ggaggaccaa ataatgctgc
    33121 tcgtccgcga tcatggcgcc gagatcggct tccgtatagc gcaccgtggt gaggttgaac
    33181 tggtttgtcc gctgatggag ctgcgcgacc cgagacagtg tcgcggaacg gaccggcccg
    33241 acatggagcc gctgctcgag cccgcggagg tagtctgaga ggctgacggc ctgcgcacga
    33301 aactgcctgc tggcgcgctc ggcggcgtac atctcggccc gccgctcgtc ctcagccgtg
    33361 agccgcaccg gccacgtcac ggcaaggctc cgcagccaca gcggacgctg ggccggatcg
    33421 gcgggcatct cgggcaccac cacctccgga cacacttggc gcatggcctc acgctcgtgc
    33481 ggactgtcgt cgagaaaaag gaagctgtcg agcccgagat tgagctcggc ggcgagcttg
    33541 cggatgttct cgggcttcgg gttccagtcg atggcggtcg ctgcaaagtc gtcggcccgc
    33601 aataccattc ccgggtggcg atcgaagacc gtgatggcat cgggatcgtt cttgctgaga
    33661 gcggcgagga gcatgccctg ccgcttcagc cgcaggcatt cctgctggaa cgtgctgaag
    33721 gcattgccgg gaaactcagg accgcacacc aatgcgccga gaccgtcgtc gccgtagacg
    33781 ccgccccaga gcgtattgtc gaagtcgagc gccagaacct tcagagggcc gcgcttcaac
    33841 agatgccacg cctcggcaaa tctcctggcg agcgcccgcg atgctgcagc ggaataggcg
    33901 atgcggccgt agtaccagag cttgggatcg gtctgttggg ccagcggcag cttggcgagc
    33961 gcggagtctg cgtcgcagac gacgatccgg ccagatcgct ccgcagcatc cagaatgcgc
    34021 tgattgacga gatcgatggc gccccgcagg ccgagggcat ggcggcggtc gagcaagccc
    34081 gccgtcggcg ccggggcgga cggaatggtg ttgatgagca gcggatgttg agcgcgctct
    34141 gcaaaggcgg tcaaagcgga ggcgagaaga tcagcccgct cgcggaccag ctccaggcct
    34201 tcgtctgccg gacggcgcca gtcgcgtgca gcgaatccgt caaaatccat gagcagaacg
    34261 agcccgtcgc aatccgcggc cgggcggagc agctcggcga ggccttcacc gaaattggcc
    34321 tcgctgaccc gagcctgcca gccttgcgcc gcaaaggcag ggacgagatc ctgcgccagc
    34381 gtatgagtgg tcgaaaatcc acacaggcga attcgcgcga tctgcaaacc ttggcggtag
    34441 tgatcgagtg gagctgccgc agacgtcagc acacgcgggg ccacgtcatg aacggagagc
    34501 gccagctccg cggcctcgtc gttccagccc agggcctgca gccgcttcac gatcgcggcg
    34561 aggacatcgc cgtcgccgct ctcgcgatgg cacagcctcc gatggatggc agcgaacgcg
    34621 cgccgctcgg ccgccgagcc ggtggcgcgg ttgagttcgt ccaatgccgc gagctcctcc
    34681 ggcaaggccc tgacgagctc gcccggcagg acatgcccag ctaagaccag cgcacgagag
    34741 agccagctcg gaacacgcac gtggtgcgct tcgagatcct ggagcatcgt cccgacgcgg
    34801 tccggcgcga cgcgggtcca atcgaccccc tgcaagagca cgcccgcgcg caggcagtcc
    34861 gccgccgacg caatagcagc ctcgaccagt tggtcggctg tcgtcggagt ccgcgcgccg
    34921 gtctcccgcg ggcgatcagc accctggtgt gcatcgctga gtgacacgcc ccctcctcac
    34981 accatcactt cgccgccggt gaccagcagg ttctcgcccg tgatgaatgc ggcataggga
    35041 gaaaccagca tcagcactgc accggcgacg tcctcggtgg tcgccagccg ccgcaggggg
    35101 gttctcgcca caaccatctg ccgcacgcga tcaggcacgt tggccacgag atcggtatcg
    35161 accatcgacg gggacaccat gttcaccctg acaccctgcg gcccgagctc ctgtgccagg
    35221 ttcttgctca tggcgagcag tgctgccttg ccggtgacat aggggagcca gtgatgcgct
    35281 gtgcggccca gcgccgccga ggtcaggacg ttgacgatca cgccacggct cgccttgagc
    35341 gcgggcaatg cagcctgtgt gacccgaaat gcgctgccga cgattgcgtc gtgcgctgac
    35401 gacatatcgt cccagccgag ccggtcgaac gaggccgaat gaattttcgg accggcatta
    35461 ttgacgagaa tgtcgagccg gccgtcgtcc gcaaccgccg caaggaggcg gtcgacgtcg
    35521 tgctggtcgg tgacatcggc cctcacgatc cggcagcgcc cgcctgcggc tgaaatctct
    35581 gccgccagat cgtcggctgc cgcgctgctc ttataataat tgatccacac gtgggcgccg
    35641 tgccgggcga gaagtcgcgc cacggcacga ccgataccgc gcgaggcacc cgtcacgaga
    35701 gccttgcggc cggccagcag tccctctcca cgaggcgtcg actccggctc cgggtggctg
    35761 tcttctgcga gccggagcac cttgacctgg gcacggccgc gcagaacgca ttccccgtgc
    35821 tggttgagga tttcggtgca gagcgtaacg aggcgggtat cgcgatcgat cttctcgact
    35881 gtggcgcgcg cctcgaccgt gtcgccgatg aaaacgggcc gcgtgaactc gatggtctgc
    35941 gaagtataga gcgcgccgcg ccccggcagc ttggtcccga tcaatgtcga aagcaggctc
    36001 gcgtgcagaa agccgtggac gactctttgc gcgaattctg tccgggccgc gaactcttcg
    36061 tcgaggtgca gcgcattgta gtctcccgac agccgcgcaa acgcagcgac atcgtcggac
    36121 gtgatcgtcc gcgacacgct ttcggtctgc cccacctgca gctcgagaag gcggtcctgg
    36181 ctgcctgtca tctgtccacg atcaagtgag tcctgcgacg gggttagcca gggagtcggg
    36241 ggaagcactt gacctcggtt aagcgcacgc ctgagcgact aggctagacg gacggattgc
    36301 ggcggtcgcg cgccgggcca tcgggggagt ggctcggcgg cttcgtcgcg atcgttgcgc
    36361 atatcgcgcg ttcaacgagg gaaagcgctt catgccatca acgttcgaga cggtcgccgg
    36421 gatcattgct gcgaccagcg acattccgat cgagcagatc accccggaca gccacgtcat
    36481 gaaggatctg gagatcgaca gcctggcgtt cctcgacatc accttcgaga tcgaccggcg
    36541 gttcggtatc gaagtgccgc tcgatcgctg gctcgagcag gtcaacgagg gaaaggtcaa
    36601 aagcgaggac tacttcgttc tgcgcaacct ctgtgcacag atcgatagcc tcagggcggc
    36661 cgcggcctga cctgctgatc gggccatgct tctcgagcgc ttccagatgc tggatcgggt
    36721 cgatgcgttc gacagggagg gcgcgtcgct gagggccttc gcgtccgtgc ccgaggaaag
    36781 cccgatcttc gaggggcatt ttccagggca tgcgctcatg ccgggcgtgc tgcttctgga
    36841 ggcgatggct caagcctcgg gctatctgtt gctggcgctc ggtggctttt cgcgcatgcc
    36901 attcttcgcc aacgtcaagt cggcgaactt caggtcgttc gtgctgccgg gcgaccagct
    36961 cgtcatcgag gcggagcgtg tgcacgccgg gtccggctac gctgtcatga gctcacaaat
    37021 tctgcgcggc ggcacgcgcg tcagcgatgc gcagatgacc ttccggcagg tgccgttccc
    37081 ggtgcccgcg ctcgagcagc atgtccggcg cgagggggag cgactgggct tcttcggatt
    37141 ggcggacagc cgatgagcgc cctgccccat caggtctgga tcacggggat cggccttgtc
    37201 agctcgctgg gcgaaggtgt cgaggcccat tgggaatgtc tcaccggcag cgcgcagccg
    37261 cggccggtgg tcgacgaaac gcgctttgcc ccttacccgg tccaccccct gacgaagctc
    37321 gacatcgcgc gacagatccc caagacgagc gatcaacggc aaatggagca gtggcagcgc
    37381 accggcgtct acgctgcggg tttggccctg actgacgccg ggatcgcggg cgacacggag
    37441 ctcctcgatc gcaccgacgt ggtcaccgcg gctggcaacg gcgagcgtga cttggagctc
    37501 gatgcgcgag tgctggagac gatcgggggt gaggcgcagc cggatgtcta tctgaacaag
    37561 gcgcttgcga ccgggctgcg gccgacgctc tacctcggcc agctttcaaa cctgctggcg
    37621 ggaaatatct cgatcatcca caaggccagg ggctcgtcgc gcacgttcaa gggtgaggag
    37681 atggcgggag tcgcggcggt cgaaaatgcc gtgcgtcgca tcgcccacgg cgaatcggac
    37741 gtcgttctgg tcggtgccgc actcaatgcc gaacgcgagg atctgctgct cggctacgag
    37801 ctcgacggct ccctgcttcg tggcccgttc cgttccgtct gggtgcgcga cgccaacggc
    37861 ggcttcgtgc cgggcagcat cggtgtcttc ctcgtcctcg aatcggagct gcacggcgct
    37921 ttccggggcg cgacgccgta cgcagccatt acgggcatcg catcggatcg ggcgcgccgc
    37981 gaagccgagc ccgtttccaa taccgcaatg gagcttttcg atcgtatcgt cgatggcttg
    38041 cgtccaggac cgctgccggt cttgagcggc gccagcggcg tggagccgat caccagccag
    38101 gagcgggcct tcctgaaaca actcgaatcg cgcgattggc ccctggccgt tcgcgcctat
    38161 ggctccgtcc tcgggcattc ggtcgaagcg catttcatta ccggcgtggc gctcgcggca
    38221 atggcggtgt cgcgacggag cttctttgcg ccattcgatg cgtccgatgt cgagcggccg
    38281 ttccagggcg tgccgcgccg tgtcctcgtc accggcttcg gccactggcg cggcgaagga
    38341 ctggcactcc tcgaagcggc ggattgggac tgaaatggcc aggcccatcc gagatcatct
    38401 ggggcgccgc gtcgtcgtcg tgacgggcat gggtctcatc acggcgcttg gacgcggcaa
    38461 ggccgagaac tgggcaggtg tcatagccgg gcgatccggg ctgaagcgca tctcgcgctt
    38521 cccgacggaa aacctgcggt cgactgtggc gggaaccgtc gatttcatgg acgtcaaccc
    38581 gtattccgcc cacgacctgt cgctggcgat ggcgatcgca acggccgagg aagccctcgc
    38641 agaggcccgc atcgagagcc gcgcgtttcc aggccctttg attgtcgcga caccaccctc
    38701 cgagttcgaa tggccgctgc tccggaagtt gcatgacgaa agccccgagc cggagcgcag
    38761 tggctacgcc cgcctgctgg cggcggcacg caccggcaat taccgcgaca tggcgcgtca
    38821 tctgaggttc gccagcatcg ccgaccggct gaaggagcgc ttcggctgcc gcggcgagcc
    38881 gctgtccatc tgcacggcct gcgcctctgg aacgacgacc atccagctcg ggctcgaggc
    38941 gatccgccgc ggcgaggcgg atgccgcact gtgcatcggc acggatgcta cggtgcaccc
    39001 ggaaggcttg atccgcttct cgcttctgtc ggcgctctcc acacggaacg atccgccgga
    39061 gaaggcctcc aggccgttct cgaaagatcg cgacggcttc gtcattgccg agggttccgg
    39121 cgccctcgtc ctggaagctt atgagagcgc ccgcgcccgt ggcgcaccga tcctcgccgt
    39181 ggtgcggggt tgcggtgaga aggcggacga ctaccaccgc acgcggtcgc gcccggatgg
    39241 cacggccatc atcggcgcta tccaaaagac gctcgacgat gccggcgtcg cgccggacga
    39301 gatcgactac attaacgcgc acggcaccag cacgccggag aacgacagga tggagtacct
    39361 gtcgctcaag gccgtgctgg gcgatcgcct gcaccaagtt ccgatcagct ccaacaagtc
    39421 gatggtcggg cacacgctga tcgccgccgg atcggtcgaa gccgtcttct cggtgctgac
    39481 gctccagaac ggcctgctgc cgccgacgat caattacgac attcccgatc ccgaaattcc
    39541 cttggacgtc gtgccaaacg aggcgcgcgc cgcaaaagtc aggacggtgc tgtcgaactc
    39601 gttcggcttc ggcggtcaga acgcgtgcgt cgtcttctcg ggagagccgg aatgacagtg
    39661 aatccaacga cggcgccgcg ggaagtcgat gaggcagggc gcacctccat gcgcgccatc
    39721 gaggtgcggg gggagcggca gcttgcgatc atcgaaacgg agcggccgcc agcacccggt
    39781 ccaggtgaag tgcaggtgcg catcggcgcc gttgctttga accacatcga tgtttggggc
    39841 tggcgcggca tggcgttcgc gcgtcgcagg ctgccgctca tcgccggtgc cgaggccgcc
    39901 ggcgagatcg tgaaggtggg accggccgtc gagcagttgc ggccaggcga catcgtcgcg
    39961 ctttacggcg cccacacctg cgggatctgt ccagcctgcc gcgagggccg cgacaacttc
    40021 tgcacggcgc aggacaatat ctacggcttc catatcgacg ggttcgcgcg agagctgatg
    40081 aatatcccgg cgcgccttgc cgtccgggcg ccctatggcg tcgacgtgac gggtgctgcg
    40141 ctggcagcca tcacctttgg cacgccggag catatgctgt tcgataacgc ccggctgagg
    40201 gagggcgaga cggtgctcgt gcacgcgggc ggcagcggca tcggctcggc tgcgattcag
    40261 ctcgccaagg cggccggggc gacggtgatc acgaccgtcg ggagcgacga gaaggctgcg
    40321 aaggccaaag ctttgggcgc cgatcacgtt atcaactacc gggaagagcg cttcgaaagg
    40381 cgcgttcggc agatcacggg tcggcgcggc gtcgaggtgg tgttcgagca cgtgggctca
    40441 gacacgtggg agggcagcat gctttcgatg aagcgcggtg gccgcctggt gacgtgcggc
    40501 tcgaccaccg gcatcagcgc caagatcaac ctcttcatgc tcttccagca acagcttcgg
    40561 ctaatcggaa gcttcggctg cacgatggcc aatatgcgca gcgcaatggc caagatggcc
    40621 cagcgccaag tgcggcccgt catcgatacc gtgatcccga tcggtcagat cgaaaggggg
    40681 ctgcagcggc tcgagagccg tcaggtattc ggcaagatca tccttacatt gtgacatgga
    40741 gcggggccgt ttcgatgcca ggaaatccgc gagaagtctg ggtcacggga atcgggctcg
    40801 tctcctccct cggcgaaggc atcgaagctc acgctgccct gctctcaccc gccgcgcgtc
    40861 cggccccaat actgaacgag agcgcctatg ccccatattc ggtgcacccg ctcgcgccgc
    40921 tcgatctctc aaagcaaatt ccgaaaaaga gcgatcagcg gcagatggag ggctggcagc
    40981 gcatcggcgt ctacgcggcg ggcctggccc ttgctgatgc gggcatcgcc ggcaacgcgg
    41041 agctgctcga ccggacaggc ctcgtcgtcg ccgccgggag cggcgagcgc gacacggcgg
    41101 tcgattgcac cgtgctcgaa aagctcgacg gccgggagga tacaggcgtg ctggccaagg
    41161 aggtcctgcc aagcgcgctc cgtccaaccc tgtttcttgc tcagctctcc aaccttctcg
    41221 ccggcaacat ctcggtggtc cacaacgtca ccggctcgtc gcgcaccttc atgggcgagg
    41281 agatggccgg gctcgccgcc gcagagaatg cggtgcgccg cattgcggcc ggccagggcg
    41341 acctcttcct cgtcggcggc tcgctcaacg ccgagcggga ggatctgctg ctcggctacg
    41401 agcttggcca caacttgtgg cgcggaccgt tccgtccggt gtgggagcgg cacgaggcgg
    41461 gaggcggttt cgtgccggga agcgtcggag cattcctggt gctcgaagcc cgcggtcatg
    41521 ccgaggcgcg cggggcgagg ccttacgcga aagtgacagg cgtcgtttcc gatcgctgca
    41581 atcgcaccca tggcgaagcg gcagaattgg gactacggct gttcgcgacc ctgtgcagcg
    41641 gcttgtcgga tgggccattg ccggtgctga gcggcgccag cggggtggca cctgtgacgc
    41701 agcaggagct ggacttcctg cgagctttgg attcgggcgg cttcgatccg atcatccgcg
    41761 cctacggcag tctcgtcggg catggcgtcg aggcccatct cgccaccgga atggccctcg
    41821 cagcgctaac gatctcgcgc caaaacttcc tgccgccgct cgatgattcc ggggtcgagc
    41881 gcccgttcga aggcgacccg ggacgggtgt tggtgacgag cttcggccat tggcgcggtg
    41941 aagggctggc gctcatcgag gccgttgccg aagggggcac acgatgaggg acagcaaagg
    42001 gcggcccgtg gtcgtggtca caggaaccgg cgtcgtcacc tccctggggc tcggaaaggc
    42061 ggagaactgg gcgaagctca catccggctg ctccggcatc cgccggatca gccgctttcc
    42121 gacggatggt ctgcgtacca cgatcgcagg cacggttgat tttttggatg tcacgccgtt
    42181 ttccgctccc gcgctgacca tggccatggc ggctgcagcg gcggaggaag cgctcgcgga
    42241 agcggagatc ggcgagaagg gagcgtttcc cgggcctctg ttcctcgcca cgccaccagc
    42301 cgagctcgag tggccgcagc gccggctgct gtgggaagca cacgctggcg gcgagctcac
    42361 gggctataag cgtctgctcg cggcggcacg cgaaagccgc cacgcggacc ttcaggaaaa
    42421 gttccagttc gcgagcgtcg ccgagcacct ggcatgccgg ttcgggacgc gtggcctgcc
    42481 ggtctccgtg tcgaccgcct gcgcctcggg cgcgaccgcg atccagctcg gagtcgaggc
    42541 gatccggcgc ggggaaacca atgccgcgct gtgcatcggc accgacagct ccgtgcagct
    42601 cgaggcgctg atccggttct cgctcctctc tgcactgtcg acccgcaacg atccccccga
    42661 agcagcatcg cgtccgttct cgaaggacag ggacggcttc gtcatggcag agggcgccgg
    42721 cgcgctggtc ctggagtcct acgagcgggc ccgcgcccgc ggcgctcaga tcctcggcgt
    42781 cgtcaaaggc tgcggtgaga aagccgacga ctatcaccgc acgcgatcga ggcccgatgg
    42841 caccgctatc atcggtgcca tccgcaatgc catcgaggac gccggcatcg cgcctcacga
    42901 gatcgactat atcaacgccc acggcaccag cacgccggag aacgacaaga tggaatacct
    42961 gtcgctcaag gccgtcctgg gcgaccactt ggagaagact ccgatcagct ccaataaatc
    43021 catgatcggc cacacgctgt cggccgcagg tgcgatcgaa gccgtctttt cgctgctgac
    43081 catccgcacg ggcgtcgtgc cgccgaccat caattaccgc aatcccgatc cagctattcc
    43141 gctcgacgtg gtgccgaacg aggcgcgccg ggccacggtc agaacggtcc tgtcgaactc
    43201 gttcggtttc ggcggccaga acgcgtcgct gatcctggcc gcactatgat cgggggaaat
    43261 cgtgatcgca tctcaatggc attttcgcgt catcgcttgg cgcggcgagg cgcaccccag
    43321 aagccgttcc gcctcatctt ccgacaaggt cggagcggtg gtggccctcg ccgcactggc
    43381 cgccgtatgc gcgaccagca cgctgccggt catcgaagcc aagcaggaag cacctgaagc
    43441 ggcagtcgcc cgcaccggtc cgctgaccga tcgcgagacc ttcatcggcg cctatacagg
    43501 cgcgccctac acgtattcga gcaacctgca tttccagagg ccggacggct cggaactcac
    43561 gatcaacgac gtggcctggg acggcaagcc gttcgacgat cccatctatt acggcgcgag
    43621 ggtgatccgc tggaccgcca tgtcccggat cggaagcatg ttcgacttca cgcacgggaa
    43681 ggcctattcg cagcccgagc aggaggtgag cgttaccggg aaaggcgagc cagagacgaa
    43741 gaaaatcagc gacatcttcc ataagctcga gttcacgcac gggcacaaca tgctgaccct
    43801 gaacgggttg ttccggctgc cgtggggcac cagcgcgatc aagccctacg tcggcctcgg
    43861 ggcaggcatc tcagttccgc acacggaggt gcagttccgc ggcgagcgcg ggcgcacgta
    43921 cgaatatcag ttcaccggcc ctgttctgca ggtggtcggc ggcttggagc tccgggtgcc
    43981 gcggctgtcc tacttcgtag agtacaaatt cacgattgcg ccgtaccgcg cgccgctgca
    44041 cctgcgtgac ggcagcctgg ccttcgcgga tctctggcac cagttcctgc gctggtggga
    44101 gggaagcgag ccggccggcg gctggctgac gacccgcctc acgagccatc aggtgatcgc
    44161 cggcatgggc tatcgcagtg gcaatgttct agccgcaggt ccgtagcccg atactgtcct
    44221 tgccggaccg cagctggtga gcagatcgac ggcacgacga caggcagtct gcactcaatc
    44281 agccgcgtcc tgttgtcagc ctgtgccaga tcgcatcgac ccgctgcacg acctccggac
    44341 tcatcgtcag cacgcggccc cactcgcgct cggtttcggg gccgatcttg ttcgtcgcgt
    44401 cgatcccgag cttgccgccg agccccggct tcggtgaggc gaagtcgaga tagtcgatcg
    44461 gcgtgtccgc gatggtcacc agatcgcgcg ccagatcggc ccttgtggag atggcccaga
    44521 tcacatcggc ccaattgcgc acatcgatgt ccgggtccac caccacgatc atcttggtca
    44581 gcgtgaactg cggcagcgcg gaccacagcc ccagcatcag ccgccgggcc tgccccgcat
    44641 agcgtttgcg gatggagacc acggccatac ggtaggagca ggcctccggc ggcagatgga
    44701 aatcctcgat ctcgggaaac tgcttgcgca gcaacggcac cagcaggcgg gcgaagacct
    44761 caccgatcac cgacggctcg tcgggtggcc ggcctgtgtg ggtggagaga tagatcggcg
    44821 cgcgccggtg cgtgatggcc gtcacctgca tcacgggaaa cggctcgacc gagttgtaat
    44881 agccggtgtg atccccgtac ggcccttcgg gcgcggtctc gccgggtgaa acggtcccct
    44941 cgatgacgat ttctgcgtcg gcaggaacgg gcaccggcac ggttatcccc ggcgcaaccc
    45001 gcgtgctccg gccacggagc agacctgaga aggtgagctc ggacatggct tcgggaattg
    45061 gcatggcggc ggcgagcatc agagccggat cggcaccgat ggcgatggcg atcggcatcg
    45121 cctcaccctt cgcccgccat tgcgcatgat ggcgcgcgcc acccctgttt gccagccagc
    45181 gcacgatgag gcgatcgcga cccagcacct gcagccgata gacgccgacg ttggttcggc
    45241 ccggggcgta gtcgcccggc ggcgatgtca cgacgagcgg ccacgtgatg agcggcgccg
    45301 gctcgcccgg ccagcaggtt tggatcggga gtgtcgatag atggacccgg tcaccctggc
    45361 agacgacctc ctgcacgggt gccgatcgtg cggccaccga gcgggtcgcc agtgcggcgc
    45421 gcagcaatgg ccatcggcga aatgcatcgc ggaaatcgct cggcgcctcg ggctggcgca
    45481 gccacgccat cgcttccccg agggcatcca gaccgtcggg cgtgacgcca aggcccatgg
    45541 ccacgcgctc gcgcgttccg aacaggttga ccagcaccgg aatgtccgag gttcggccgt
    45601 cagccatgac ggctcgctcg aacaggagcg cggggccgcc ggcccgcaac atgcgccggt
    45661 gcacctccgt catctcatga accagcgaga ccggctcgtc gacgcgcatc agctggcccg
    45721 accggtcaag gtgggcgatg aaggctctga gatcggggaa tggcggcagg ctgcgtagcg
    45781 acacccaagg cacctccggc gcgatcctgg gcggcggccc gggagcgcgc cttaaccccg
    45841 gtcaaggagg cgcacggatg actgacctac caagcacctc atgccgaacg cacctcagct
    45901 cccggccctt ctgaccagcg ccatgcgcct gctgccaacg cagccgctcg agctggcgct
    45961 cgaccggctg acgaaatcga tcgcacagcg tcatcctgac ctgttcgatc gcctgggtga
    46021 gcattcccac aaggccatcg gcatcgctcc aacggatgtt ccggtgatct tcgtgctgat
    46081 gccggatcgg gaagcgccgc gtgtcgaggt gaagcggacg gactcggcgc ccgcttgcga
    46141 tgcatcgatc gtcggaccct tattcgccct cgtcgacatg gccgaggggc ggctcgatgg
    46201 cgatgcgctc ttcttctcgc gggacattcg catcgagggc gacgtcgagg cggtccttgc
    46261 actgcgcaat gcactcgacg gtgaagggct cgatctcatc agggaggcaa cggcaccgct
    46321 cgggatactc gccgccggcc cggagctgat cggccggcgt tgcctcaacc tcctgcgatc
    46381 ggctcttcag accgcaccgg cagaggagac cccgttttga agccacagtc ccgtctcgag
    46441 ctcgtgtgcc ccgccggcac gccaggggcg ctgcgggtgg ccgtcgatgc cggagccgat
    46501 gcagtctatt gcggcttccg cgacgaaacg aacgcgcgca atttccccgg cctgaatttc
    46561 agccgcgagg agctgaagga agcagtcacc tacgcccacg cccgcggctg caaggtgctg
    46621 gtggcgatca acaccttccc gcgggcggga catgtggcgc tctggcagag ggcagtggat
    46681 gatgcggttg cgctcggtgc cgacgctctc atcctgtgcg acatcggtct gctcgactac
    46741 gcggcgacga agcatccgaa agcgcggctc cacctgtcgg tgcaggcggc cgcggcgaac
    46801 ccggaagcta tccgctttta tacggcatca ttcggcgtga agcgggtcgt gctgccgcgc
    46861 gtgctcaccg tctccgagat cgccagcata aacgagggta tcgattgcga gacggaagtg
    46921 ttcgtcttcg gcggattgtg cgtcatggcc gaggggcgct gctcgctgtc gtcctacgcg
    46981 actggcaagt cgccgaacat gaatggcgtc tgctcgccgc cgagccacgt cagctatcat
    47041 gaggagggcg acgagctggt cccccgcctc ggcggtttca cgatcaaccg gattccgcgc
    47101 tcggagcctg cggcctaccc gaccctgtgc aagggccgtt tcgtagcggg gaagtcatcc
    47161 ggctacatct tcgaggaccc cgtcagcctc aacgcgggca cactcgtgcc cggtcttgca
    47221 aaagccggtg tgacggcgct caaaatcgag ggtcgccagc gcgggcgcgc gtacatcgcc
    47281 gcggtggtca aagcgttccg gcaggcggtc gacgctttgg ccgaggggcg gtccgtcgac
    47341 ctcgaccggc tgctcgcgct tgccgagggc cagtcgtcga cccaaggcgc ctacgccaag
    47401 acctggaggt aggtgcagat gtcaaatccc gtgagaatta cgctcggccc cgttctcttc
    47461 aattggcagc cggaggcatg gcgcgatttc tacgcccgga tcgcggacga ggccccggtg
    47521 gatcaggtgt acctcggcga ggtggtgtgc tcgaagcgca tgcccttctt cgacgagctg
    47581 atcgtcgatg t
//
LOCUS       DASVDU010000154         6130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_123000, whole genome shotgun sequence.
ACCESSION   DASVDU010000154 DASVDU010000000
VERSION     DASVDU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123000"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..918
                     /locus_tag="VF226_09905"
     CDS             370..918
                     /locus_tag="VF226_09905"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX7074342.1"
                     /translation="MMETAHYPAEIFWSDDDSGYIAIAPDLPGCSAFGRTQQEALEEL
                     QTAIEGWISAARAVGNDVPPPSRPSAASPSGRVLLRMPRSLHAQLAKQADNEGVSLNQ
                     YIVSLLSRRAAERTIMETLEDWLLGRRQWRRWRSIGSDLVAHFEKRGYEGSDQAFYVL
                     ISAPEYTASTADLFHKTRTPPG"
     gene            973..1236
                     /locus_tag="VF226_09910"
     CDS             973..1236
                     /locus_tag="VF226_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074343.1"
                     /translation="MANPTRYTFDLKEVATALIRAQGIKHGKWILGFEFNFGAGQIGP
                     SPDDSLPAGFIRVQNALLTRQPDDAPESNLVVDAEKVWSQGQG"
     gene            1454..1966
                     /locus_tag="VF226_09915"
     CDS             1454..1966
                     /locus_tag="VF226_09915"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7074344.1"
                     /translation="MDWAAFNVDFLTGAGRDALWWQTCIKAILVFLFGLVLIRLLGRR
                     AFGMQTPLDIVLAIIIGSNLSRALTGNAPFLPTLAATALLVVLFWLLDQAAIRWHWLS
                     WLVKGSPVLLMRDGRFERKNMLRYGISEGDLEEAARRSGLRDLGSVQEAVFERNGQIS
                     TIRRERSRRG"
     gene            2163..2732
                     /locus_tag="VF226_09920"
     CDS             2163..2732
                     /locus_tag="VF226_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HEX7074345.1"
                     /translation="MDETLAARQRLEGFLFLAAFGLCIPAANWLIGNAGTVCPADSPC
                     LIPVAPGLMAPSGVLMVGLALVLRDLVQRRLGLGWAGGAILTGAILSALFAPPSLVVA
                     SAVAFLLSELADLAVYTPLQRRRLVLAVVSSSVVGLVVDSAVFLYLAFGSLEFLAGQV
                     IGKAWMVLFATPLIAWLRRRDARLGLIPA"
     gene            2744..3205
                     /gene="queF"
                     /locus_tag="VF226_09925"
     CDS             2744..3205
                     /gene="queF"
                     /locus_tag="VF226_09925"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX7074346.1"
                     /translation="MSTDTSKLTQLGRPSALPESPEAAQLERVPNPHPDTPYVARFTA
                     PEFTSLCPVTGQPDFAHLVIDYVPRDWIVESKSLKLYLSAFRNHGAFHEDCTVAIGKR
                     LVALLDPHFLRIGGYWYPRGGIPIDVFWQTGELPKGVWLPDQGVAPYRGRG"
     gene            3227..3556
                     /locus_tag="VF226_09930"
     CDS             3227..3556
                     /locus_tag="VF226_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEX7074347.1"
                     /translation="MSYVYLCLAIVAEVIATSALKASEGFTRPVATIITTAGYLAAFY
                     FLSLTLRTIPIGLAYAIWSGVGIVLISVIGFVLFRQSLDMPAILGLALIIAGVVVINL
                     YSETMPR"
     gene            complement(3563..4117)
                     /locus_tag="VF226_09935"
     CDS             complement(3563..4117)
                     /locus_tag="VF226_09935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX7074348.1"
                     /translation="MMRPVLLLIDFQKGFDFIAARTRRNNIEAEARAMALLTLWREKG
                     WPVIHVRHDSTEPQSPFRPGSVGNRAMGFAEEQEGEAVVRKTVNSAFIGTDLAERLDA
                     LGRPQVVVAGATTDHCVSTTVRMGANLGYTMTLVDDACFTFDRMAPNGDVIPAEAIHA
                     AHVASLSGEFARIATAAAMIAELK"
     gene            complement(4114..6087)
                     /gene="thrS"
                     /locus_tag="VF226_09940"
     CDS             complement(4114..6087)
                     /gene="thrS"
                     /locus_tag="VF226_09940"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7074349.1"
                     /translation="MAEITLTFPDGAKRTFPKGITGKALAETISKSLAKKAVAMSVDG
                     RLTDLADPIEADAEVKIITRNDPEALELIRHDAAHVMAEAVQELFPGTQVTIGPVIEN
                     GFYYDFFRNEPFHPDDLAKIEARMHEIIKRDKPFEKEVWSREKAKAYFREKGESFKVE
                     LVDAIPENEDVKIYRQGEWLDLCRGPHMTSTGQIGKAFKLQKIAGSYWRGDSSKAQLQ
                     RIYGTAWANEQDLAAHLKMLEEAEKRDHRRLGREMDLFHFQEEGPGVVFWHPKGWTLF
                     KTLENYMRRRQGAVGYREVNTPQILDKSLWETSGHWETFREHMFTTTTEDEREFAIKP
                     MNCPGHIQIYKHGLRSYRELPYRLSEFGCVHRYEPSGALHGLLRVRGFTQDDAHIFCT
                     EDQIADECLKINDLIMAIYRDFGFDQVVVKLSTRPEKRVGTDEQWDQAEKAMMAVLER
                     IAADSGGKVKTGINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFNLPGRFGAFYID
                     AEGQRRTPVMIHRAIFGSLERFVGIILENYAGHLPLWLAPVQATVCTIVSDADGYAKE
                     VQRELQEAGLRAEVDLRNEKITYKVREHSLAKVPVLLVVGKREAEERTVSIRRLGSQQ
                     SRVMPLSEAVRMLVEEATPPDLVKSEARVTEAA"
BASE COUNT         1067 a         1960 c         1871 g         1232 t
ORIGIN      
        1 cggttgccga acccggctcg aattgtcgct tcccacgccg ggcggtgcgg tttgtgctgg
       61 ccacttcccg ttagagcctt caacgactat ctgttaatgt ggtgatactg catgtagtat
      121 caccacaatg gcgcagcaag gtaagctgat ccgggggctc ttgaacaacc cgaaggatgt
      181 tcgggtcgag gacgcctgca agattgctcg atgggcaggc ttcgaagaac agggcggtaa
      241 agggcggcat aggttctttt caaaaccagg attcccctta gtcctcaatt tccaggaccg
      301 cggggacggt agaataccta cgtaccaagc caagcagttg attgatgtac tagaagagct
      361 gggccttaca tgatggaaac agcacattac cccgctgaga tcttctggag cgacgacgat
      421 agtggctaca tcgctatcgc gccagacctt cctggctgct cagcttttgg tcgcacccag
      481 caggaagcac tggaagaact acagaccgcg attgaaggct ggatttcggc ggcgcgcgcc
      541 gttggcaatg acgttccgcc tccgtcacga ccttccgccg cgagccccag cggtcgcgtt
      601 ctccttcgga tgccgcgcag tctccatgcc caactggcaa agcaggccga caacgagggg
      661 gtcagcctga accagtacat agtttcactc ctatcgcggc gtgctgctga gcgaacgatc
      721 atggaaacct tagaagattg gttgctcggc aggagacaat ggaggaggtg gcgcagcata
      781 gggagcgatc tcgtcgccca cttcgaaaag cgcggatacg aaggttctga ccaagcattc
      841 tacgtcttga tttccgctcc ggaatacacg gcatcaacag ccgatctgtt tcacaagacg
      901 cgcactcccc ccggatgatc agcagttcct gacatctaga ggagagctcg tgaagccaga
      961 ttgaggcgca gcatggcaaa tccaactcgg tacaccttcg acctgaagga ggttgcgact
     1021 gccctgattc gggctcaggg catcaagcat ggaaaatgga tactcggctt tgagttcaac
     1081 ttcggcgctg gccagatcgg cccttcgcca gacgacagtc tccctgcagg cttcattcga
     1141 gtacagaacg cgctactcac gcggcagcct gatgacgcac cggagtctaa tcttgtcgtc
     1201 gacgccgaaa aagtgtggag ccagggccaa gggtagactg ggagttccgg acagccctcg
     1261 atttcatgtc cagaacccat ccccagaacg tcgttcatct gcgcattttc cgggtaacgt
     1321 cagctcgcgc ctagagcagc cccggcctct gcgggctgca tgaacgcctc agtccgcagc
     1381 cattggaacc aaatctgccg atcaaggatt gccgagctat ccttcgtaga acctgccgcg
     1441 ctggatgagc caagtggact gggccgcatt caacgtagat ttcctcaccg gcgccgggcg
     1501 agatgccctc tggtggcaga cttgcatcaa agccattctc gtcttcttgt tcgggctggt
     1561 tctgatccgc cttctcgggc gccgcgcttt cggcatgcag acgccgctcg acatcgtcct
     1621 tgccatcatc atcggctcga acctcagccg ggcgctcacc ggcaacgcgc cgttcctgcc
     1681 gacgctggcc gcaacggccc ttctcgtcgt cctgttctgg cttctggatc aagcggccat
     1741 acgttggcac tggctcagct ggctggtgaa gggctctccc gttctactga tgcgagatgg
     1801 tcgattcgag cggaagaaca tgctccgata tgggatcagc gaaggggact tggaggaagc
     1861 agccaggcgc tccggtctgc gagacttggg cagcgtgcag gaagccgttt tcgaaagaaa
     1921 cggtcagatc agcacgatca gacgtgaaag gagccgccgg gggtgaacca ctggggcact
     1981 ttcggcaaca gcgaaggcga taacttgccg ccgtcgagct cccaagcgac aacgtgacca
     2041 gtggcgggat cgcgaggtga tcccgtgact agggggcgca gccgcggcac gagtcggctg
     2101 ccgggctttg cgatgccgct gcgggcctgt aaaacggctt tcaagaacaa ggagatgagg
     2161 tgatggacga gacgctcgcc gcgcggcagc ggctcgaggg ctttctgttc cttgccgctt
     2221 ttggcctctg cattccggca gccaactggc tgatcggtaa tgcggggacc gtctgcccag
     2281 cggatagccc gtgcctcatc cctgtcgcgc ccggcctgat ggccccgagc ggcgtgctca
     2341 tggtcggctt ggcgctcgtg ctgcgcgatc tggtgcagcg gcgcttgggc ctcggctggg
     2401 ccgggggcgc gattctgacc ggagcgatcc tctctgccct ttttgcaccg ccctcgctgg
     2461 ttgttgcgtc tgccgtcgca ttcctcctgt ccgagctcgc cgacctcgcc gtctacacgc
     2521 ctctgcagcg gcgccgtctg gtgctggcgg tcgtgtccag cagcgtggtc gggctggtcg
     2581 tggacagtgc cgtctttctc tacctcgcct tcggcagcct cgagttcctg gctgggcaag
     2641 tgatcggcaa ggcctggatg gtgctcttcg ccacgcccct gattgcttgg ctgcgccggc
     2701 gagatgccag actgggcctg atccctgcct gaaagatttc gctatgagta ccgacacctc
     2761 gaaactgacc cagctcggcc gtccgtccgc cctcccggaa agcccggaag ccgcccagct
     2821 cgagcgcgtg ccgaatccgc atccggatac gccctatgtg gcccgcttca ccgcaccgga
     2881 gttcacatcg ctctgccctg tgaccggcca accggatttc gcgcacctcg tcatcgacta
     2941 cgtgccgcgg gactggatcg tcgagtcgaa atcgctgaag ctttacctgt ccgcattccg
     3001 caaccacggt gcgttccacg aggactgcac cgtcgcgatc ggtaagcggc tcgttgcgct
     3061 gctcgatccg catttcctgc gcatcggcgg ctattggtac ccgcgtggcg ggattcccat
     3121 cgatgtgttc tggcagacgg gcgagctgcc gaaaggcgtc tggctgcctg accagggcgt
     3181 ggcgccgtat cgcggccgcg gatagctccc gaaaggaacc gccgctatga gctacgtcta
     3241 cctgtgcctc gcgatcgtgg cggaagtgat tgcgacgtca gccctgaagg cctccgaggg
     3301 cttcacgcgg cccgtcgcga cgatcatcac gacggcaggc tacctggccg cgttttactt
     3361 cctgtccctg acgctaagga caattccgat aggacttgcc tatgccatct ggagcggcgt
     3421 cggtatcgtc ctgatctcgg tgatcggctt tgtgctgttc cgccagtccc tcgacatgcc
     3481 ggcgatcctc ggcctcgcgc tcatcattgc aggggtcgtc gtgatcaacc tctactccga
     3541 aacgatgccc cgctgaggaa cgctatttca gctcggcgat catcgcggcc gcggtcgcaa
     3601 ttcgcgcgaa ctcgcccgaa aggctcgcga catgcgcggc gtggatcgcc tcggccggta
     3661 tcacgtctcc attcggagcc atccggtcga aggtgaagca ggcatcgtcg accagcgtca
     3721 tcgtgtaacc gaggttcgcg cccatgcgca ccgtcgtcga tacgcagtgg tcggtcgtcg
     3781 cgccggccac cacgacctga ggacggccga gcgcgtcgag gcgctcggcc agatccgtgc
     3841 cgatgaaggc gctgttcacc gtctttcgaa cgaccgcttc accctcctgc tcctcggcaa
     3901 agcccatcgc gcggttgccg acggagcctg gacgaaacgg gctctgcggc tcggtcgagt
     3961 cgtggcggac atggatcacc ggccagccct tctcgcgcca caaggtcagc agcgccatcg
     4021 cccgcgcctc tgcctcgatg ttgttgcggc gggtgcgggc cgcgatgaaa tcgaaaccct
     4081 tctggaaatc gatcagaagc aggacaggcc gcatcatgca gcctctgtga cgcgcgcctc
     4141 ggacttgacg aggtcgggcg gcgtcgcctc ctcgaccagc atgcggaccg cctcggagag
     4201 aggcatcacg cggctctgct ggctcccgag gcggcggatc gatacggtcc gctcctcggc
     4261 ctcgcgcttg ccgaccacca gcagcaccgg taccttggcc agcgaatgct cgcgcacctt
     4321 gtaggtgatc ttctcgttgc gcaggtcgac ctcggcccgc aggcccgctt cctgcagctc
     4381 tcgctgcacc tctttggcat acccgtcagc atccgacacg atcgtgcaga cggtcgcctg
     4441 gacgggtgcg agccacagcg gcaggtggcc tgcgtagttc tccagaatga tgccgacgaa
     4501 ccgttccagc gagccgaaga tggcgcgatg gatcatgaca ggagtccgcc gctggccctc
     4561 tgcatcgatg tagaaggcgc cgaagcggcc cggcaggttg aagtccacct gcgtggtgcc
     4621 gcattgccag tcgcggccga tggcatcgcg cagcacgtat tcgaacttgg gaccatagaa
     4681 cgccccttcg cccgggttga tgccggtctt caccttgccg ccggagtcgg ccgcaatccg
     4741 ctccagcacc gccatcatgg ctttctcggc ctgatcccac tgttcgtcgg tgcctacgcg
     4801 cttctcgggc cgggtcgaca gcttcacgac gacctgatcg aacccgaaat cgcggtaaat
     4861 ggccatgatg agatcgttga tcttcaagca ctcgtccgcg atctgatcct ccgtgcagaa
     4921 gatgtgcgcg tcgtcctgcg tgaagccgcg cacgcgcagg agcccgtgca gcgcgccaga
     4981 cggctcataa cgatgtacgc agccgaactc ggagagccgg tacggcagct cgcgatagga
     5041 ccgcagcccg tgcttgtaga tctggatatg gcccgggcag ttcatcggct tgatcgcgaa
     5101 ctcgcgctca tcctccgtgg tggtggtgaa catgtgctcg cggaaggtct cccagtggcc
     5161 ggaggtctcc cacaagctct tgtcgaggat ttgcggcgtg ttcacctcgc gatagccgac
     5221 ggcgccctgg cggcgacgca tgtagttctc gagggtcttg aagagcgtcc agcccttcgg
     5281 atgccagaac acgacgcccg gcccctcctc ctggaaatgg aagaggtcca tctcgcgccc
     5341 gagccggcga tgatcgcgct tctcggcctc ctcgagcatt ttgagatggg ccgcgaggtc
     5401 ctgctcgttg gcccacgccg tgccgtagat gcgctggagc tgggccttgc tggaatcgcc
     5461 gcgccagtag ctgccggcga tcttctgcag cttgaacgcc ttcccgatct gccccgtcga
     5521 ggtcatgtgc gggccgcggc agaggtcgag ccattcgcct tggcgataga tcttgacatc
     5581 ctcgttctcg ggaatggcgt cgacgagctc gaccttgaag ctctccccct tctcgcggaa
     5641 gtaagccttg gccttttcgc ggctccagac ctccttttcg aagggcttgt cgcgcttgat
     5701 gatctcgtgc atccgcgcct cgatcttggc cagatcgtcg ggatggaacg gctcgttgcg
     5761 gaagaaatcg tagtagaagc cgttctcgat caccggcccg atggtcacct gcgtgccggg
     5821 gaagagctcc tgcaccgcct ctgccatgac gtgcgcggcg tcatgccgga tgagctccaa
     5881 ggcttccgga tcgttgcgcg tgatgatctt gacctcagcg tccgcctcga tggggtcggc
     5941 aaggtcggtg agccggccgt cgacgctcat ggcaacggcc ttctttgcga gcgacttcga
     6001 gatcgtctcg gccagagcct tgcccgtgat ccctttcggg aatgtccgct tcgccccatc
     6061 cgggaaggtc aatgtgatct cggccatttt tcgcgatctc ctctcactcg ctgcatacca
     6121 cgcaggtaag
//
LOCUS       DASVDU010000155         4424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1451634, whole genome shotgun sequence.
ACCESSION   DASVDU010000155 DASVDU010000000
VERSION     DASVDU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451634"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1218)
                     /locus_tag="VF226_09945"
     CDS             complement(<1..1218)
                     /locus_tag="VF226_09945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEX7074350.1"
                     /translation="MTDPVRDRVVEVTRLGAQGDGVAETPGGAVYLPFALPGERWRIA
                     DGRPAERLTASSHRQQPLCRHFGQCGGCVAQHMTPELYRSWKEAVLVNAFAHRGIEVT
                     PRPMRLVAAQSRRRAFFGVARRGDEVVLGFREEGRHALVDLAECPVLDPAITTALPLL
                     RELARMALPPEAGGRLIVTRAQGGLDVAIENGSTRLAPEARADLARRAAAARVQRLTV
                     FGDVVYMEQTPWLTLGGVRVELPAGAFLQAVPEAEAAMIELVTQAVCRAKSVADLFSG
                     LGTFTFPVARKARVLAVDSERRSLEALEKAARGATGLKPITTRIRDLMREPLSRGELE
                     GFDAVVLDPPRAGAKAQAESLARSRVPTVVMVSCNPATAGRDARILIDGGYRLESLTP
                     IDQFLYSAHLELVA"
     gene            complement(1211..1951)
                     /locus_tag="VF226_09950"
     CDS             complement(1211..1951)
                     /locus_tag="VF226_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX7074351.1"
                     /translation="MVRTRLDRLLVARGLVATRSRARDLITRGKVTVDGVSVAKPGLE
                     ISEDAEVSISDGANEFVSRGAQKLEAALAHFGYRPSGVTALDIGASTGGFTQVLLEAG
                     ARRVYAVDVGRGQLDRSLSDDPRVISLEGTDARTLSKEQIPEPVGAIVADVSFISLTK
                     VLSVPLSFTVPGAWLIALIKPQFEAGREAVGKGGIVRDPADRERAVSIVRAWLAAQAG
                     WFVDGVVASPLLGGSGNEEFLIGARKDD"
     gene            2070..2792
                     /locus_tag="VF226_09955"
     CDS             2070..2792
                     /locus_tag="VF226_09955"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7074352.1"
                     /translation="MPTLNRRAFLAGAAAFSTYVVADRSWGQATQGPFTLPPLPYAYN
                     ALEPHIDAETMQLHHDRHHAAYVNNLNEAVRDHSELARQPLQNILANLNNVPESIRTK
                     VRNNGGGHANHSMFWLVMGPGGGGQPQGELQQAIERDFGGLDQLQSRFNSAGESVFGS
                     GWVLVAVDKDGRLELTTRPNQDTPLMEGGHVLFGNDVWEHAYYLKYRNRRGEYLKNWW
                     NVVNWQSIGERYSRAKAGTLEI"
     gene            complement(2974..3978)
                     /gene="cydB"
                     /locus_tag="VF226_09960"
     CDS             complement(2974..3978)
                     /gene="cydB"
                     /locus_tag="VF226_09960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7074353.1"
                     /translation="MAIDLPLIFAFVIALAVLIYVIVDGFDLGIGILFPFFPAEDDRR
                     RMMNSIAPVWDGNETWLVAGGGGLLAGFPLAYAVIFSALYAPIIAMLLALIFRGVAFE
                     FRFRSRRNRHLWDLGFAVGSIVAAFSQGMILGALVQGIPVANRAYAGGWWDWLTPFSI
                     LTGVALVVGYSLLGATWLIMKTEGDLQSQAYQWAYRAAIGTLAGIAFVSIITPLLNPV
                     YEQYWFSWPAILYTMLVPILVIVAAYFLFQSLAQRRTYAPFLLSISLFLLSYIGLGIS
                     FHPYVVPTSITIWEAAAPDSSLIFILLGAAFMLPLIVVYTAYSYWVFRGKVDVEGGYH
                     "
     gene            complement(3968..>4424)
                     /locus_tag="VF226_09965"
     CDS             complement(3968..>4424)
                     /locus_tag="VF226_09965"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7074354.1"
                     /translation="IGFLMALMGVWAAWMRWKGRLFDSPALLRSAVIMGPSGFIAVLA
                     GWIVTEVGRQPYTVYGLLRTADSASPIATTGVAASLIAFFVVYMVVFGAGTVYMFRLA
                     GRRPEPTEPEVPHVPIRTAGITPAPALTEKGFEEDEQEPARTQGGGRGD"
BASE COUNT          902 a         1481 c         1370 g          671 t
ORIGIN      
        1 ggccaccagc tccaggtgtg ccgagtagag aaactggtcg atgggcgtga gggactcgag
       61 ccggtagcca ccatcgatca gaatgcgcgc atctcgcccc gcagtcgccg ggttgcatga
      121 aaccatcact acggtcggca cgcgcgatct ggctagactc tccgcctggg ccttcgcgcc
      181 tgcgcgcggt ggatcgagca ccaccgcgtc gaagccctcc agctcccctc tcgacagcgg
      241 ctctcgcatc aggtcacgaa tgcgggtggt gatcggtttc agacctgtcg ccccgcgcgc
      301 ggctttctcg agcgcctcga gggaccggcg ctcggaatcg acagccagca cccgggcctt
      361 tcgcgccacc ggaaacgtga acgttccgag ccccgagaag agatccgcaa cggacttcgc
      421 cctgcatacg gcctgcgtga ccagctcgat catcgcggcc tcggcctcag gcacggcctg
      481 tagaaatgcg ccggccggga gctcgacacg cacaccgccc agagtaagcc acggcgtctg
      541 ctccatgtag accacgtcgc cgaacactgt gagtcgctgc accctggcgg cggccgcccg
      601 gcgcgccagg tcagccctcg cctccggagc gagacgcgtc gatccgttct cgatggcaac
      661 gtcgagccca ccttgcgcgc gcgtgacgat cagccggccg ccggcttcgg gcggcagcgc
      721 catgcgtgcc agctcgcgca gcaggggcaa cgcagtcgtg atcgccggat cgagcacagg
      781 gcactcggcg aggtcgacga gggcatggcg gccctcttct cgaaatccaa gcaccacctc
      841 gtcgccgcgc cgcgcaaccc caaagaacgc ccgccgtcgc gattgcgcag caacgagccg
      901 catcggccgg ggcgtgacct ctattccccg gtgggcgaaa gcgttgacga gcaccgcttc
      961 cttccagctc cggtagagct ccggtgtcat gtgctgcgcc acgcagccgc cgcactggcc
     1021 gaagtggcgg cacaacggct gctggcgatg gctgctcgcc gtcagtcgct cggccggccg
     1081 cccgtcggcg atgcgccagc gctcgcccgg cagcgcaaac ggcaggtaga ccgcgccccc
     1141 cggcgtctcg gcgacgccgt cgccctgtgc gccgagccgc gtgacctcga ccactcgatc
     1201 cctcacaggg tcagtcatct ttccgcgctc cgatcaggaa ttcctcgttt cccgacccgc
     1261 cgagcagggg tgacgcgacc acgccgtcga caaaccatcc ggcctgcgcg gcaagccacg
     1321 cgcggacgat gctcacggca cgctcgcggt cggccggatc gcgcacgatg ccgcccttgc
     1381 cgaccgcctc acgaccagct tcgaattgcg gcttgatcag cgcgatgagc caggcccccg
     1441 gaacggtgaa ggacagcggc acagacagca ccttggtcaa ggagatgaag ctcacgtcgg
     1501 caacaatggc accgacgggc tcgggaatct gctccttgct caaggtcctc gcgtccgtgc
     1561 cttctagaga gatcacccgg ggatcatccg acagcgaccg atcgagctgg ccacggccga
     1621 catcgacggc gtagacgcgc cgcgcaccag cctcgagcag aacctgcgtg aaacctcccg
     1681 tcgaggcccc gatgtcgagg gcggtcacgc ccgagggccg atagccgaaa tgcgccagcg
     1741 cagcctccag cttctgcgca ccgcgggaca cgaactcatt ggcgccgtcg gaaatcgaga
     1801 cctccgcatc ctccgaaatc tccaaccccg gcttggcgac cgacacgccg tcgacggtga
     1861 ccttgccgcg cgtgatcaga tcacgtgcgc gcgaacgggt cgccaccagt ccccgtgcaa
     1921 ccagaagccg atcgagtcgc gtccgaacca tccttctcct tgctcgttac tggcccaacc
     1981 atccttggtg taccgggcca cgccggccca tgcgccgtcc ggccgaaaga gcgcattgcc
     2041 ggactaccag tagatcatgg agttgaccga tgcccacgct caatcgccgc gccttcctcg
     2101 cgggcgccgc agccttctcg acatatgtgg ttgccgaccg gagctgggga caggcgacac
     2161 aaggcccgtt tacgcttccc ccgctgccgt acgcgtacaa cgccctcgag ccgcatatcg
     2221 atgccgagac gatgcagctt caccacgacc gccaccacgc agcctatgtg aacaacctga
     2281 acgaggcggt tcgtgaccat agcgaattgg ccagacagcc gctgcagaac atcctggcca
     2341 acctgaacaa cgtgcccgag agcatccgca ccaaggtgcg caacaacggg ggcggccacg
     2401 ccaatcactc gatgttctgg ctggtgatgg ggccgggcgg cggcgggcaa ccccagggcg
     2461 agctgcagca ggcgattgag cgggacttcg gtgggctgga ccagttgcag agccggttca
     2521 acagcgccgg ggagagtgtt ttcgggtcag gatgggttct cgtcgccgtg gacaaggacg
     2581 gccgcctgga gctcaccacg cggccgaacc aggatacgcc gctgatggaa ggtggtcacg
     2641 tgctgttcgg caatgacgtc tgggagcatg cgtactacct caaataccgg aaccggcgcg
     2701 gcgagtatct gaagaactgg tggaatgtcg tgaactggca gtcgattggc gagcgctaca
     2761 gtcgcgccaa ggccggcact ctcgagatct gatgacgctg gttgccgaat tcgtgatcag
     2821 cccggcgggt cacgcgtcaa ggcgcaattg cccagcggat cgcgtatgcc accgcggcga
     2881 cgacggccac tccggccagc cataagccaa caaaccacgc caaccggtaa agaagcccat
     2941 gtgggggttc ctgccgcggg tcgggcggct gcatcaatga tagccccctt cgacatcgac
     3001 cttgccgcgg aagacccagt aggaataggc ggtataaacc acgatgagcg gcagcatgaa
     3061 cgccgcgccc agcaatatga agatcagact gctatccgga gcggcggcct cccagatcgt
     3121 gatgctcgtc ggtaccacgt aaggatggaa gctaattccg agcccgatgt aggaaagcag
     3181 aaacagactg atggagagta agaagggtgc ataggtccgg cgttgggcaa gcgattgaaa
     3241 gaggaagtag gcggcgacga tcaccaggat cggcaccagc atcgtgtaga ggatcgccgg
     3301 ccagctgaac caatactgct catagacagg gttgaggagc ggcgtgatga tactgacgaa
     3361 cgctattccg gcgagcgtgc cgattgcggc ccgataagcc cattgatagg cctggctctg
     3421 tagatcacct tcggttttca tgatgagcca tgtcgccccg agcagggaat agcccaccac
     3481 caatgccact cccgtaagga tgctgaacgg cgtgagccag tcccaccatc cgccggcata
     3541 cgcccgattg gccaccggaa tcccctggac gagcgcaccg agaatcatcc cttgcgagaa
     3601 tgcggcgaca atcgagccca ctgcgaagcc gagatcccag agatgcctgt tgcgcctcga
     3661 ccggaagcgg aactcgaagg caacgcctct gaaaatcaga gccagaagca tggcgatgat
     3721 gggcgcatag agagcggaaa agatgacagc gtaggccaaa ggaaatccgg ccagaagtcc
     3781 acctccacct gcgacgagcc acgtttcgtt accgtcccat acgggagcga tcgagttcat
     3841 cattcgacgg cggtcatctt ctgccgggaa aaagggaaac agaattccga tcccgagatc
     3901 gaatccgtcg acaatgacgt aaatgagcac cgccagcgcg atgacgaagg caaaaatgag
     3961 ggggagatca atcgccacga ccgcctcctt gcgttcttgc gggctcttgc tcgtcttctt
     4021 cgaaaccctt ctccgtcagc gcgggagccg gcgtgatccc cgcggttcgg atcggcacgt
     4081 ggggcacctc cggctcggtg ggttcagggc gccgtccagc cagccggaac atgtagacgg
     4141 tgcctgcgcc gaaaacgacc atatagacga cgaagaacgc aatcagggaa gcagccacac
     4201 cggtcgtggc gatcggcgat gcggaatccg cagtgcgcag caatccgtac accgtgtacg
     4261 gctgtcgccc gacttccgtg acaatccagc cggccaacac cgcgatgaag ccggaggggc
     4321 ccatgatgac ggctgaccgc aggagtgccg gcgagtcaaa gagccgtccc ttccacctca
     4381 tccaggccgc ccagacaccc atgagcgcca tgaggaaccc gatg
//
LOCUS       DASVDU010000156         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_978797, whole genome shotgun sequence.
ACCESSION   DASVDU010000156 DASVDU010000000
VERSION     DASVDU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978797"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF226_09970"
     CDS             complement(<1..221)
                     /locus_tag="VF226_09970"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HEX7074355.1"
                     /translation="MSPNRDALAVTFDDGESYELSAEMLRVMSPSAEVQGHSPDQRVT
                     VARKRNVKIRELRPVGNYAVRIVFDDGHDT"
     gene            complement(249..1406)
                     /locus_tag="VF226_09975"
                     /pseudo
     CDS             complement(249..1406)
                     /locus_tag="VF226_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468279.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
     gene            1579..2175
                     /locus_tag="VF226_09980"
     CDS             1579..2175
                     /locus_tag="VF226_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7074356.1"
                     /translation="MMKGLLAFSRTVDRATGWVGKSVAWLIVAAILISAGNASVRKLF
                     DTSSNAWLELQWWLFGAVFLLCAAWTLSSNEHIRIDIVNNHLSRSARNIIELVGHLLF
                     LMPMCLVILYTSIPFFLRSWEQNEQSSNAGGLPLYPPKLLIALGFALLLLQAISELIK
                     RIAIMRGDLEEEGSSPGGHKASAEAEAKRLLEDGAQSG"
     gene            2284..4110
                     /locus_tag="VF226_09985"
     CDS             2284..4110
                     /locus_tag="VF226_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7074357.1"
                     /translation="MAAFIAENIAPIMFVALVVVLLLGYPVAFSLGAVGLIFFVIGVE
                     LAPLSDGAINLSWPLLQSLPERIFGIVNNDTLLAVPFFTFMGLILERSGMAEDLLDTI
                     GQLFGTVRGGLAFAVIFVGALLAATTGVVAASVISMGLISLPIMLRYGYDRRVASGVI
                     AASGTLAQIIPPSLVLIVMADQLGRSVGDMYQGAFVPGLVLAGLYAAYVGLVSLIWPK
                     ATPGLPPEAIGFKEKNGSRGLTSLAILTVVSSVFGWYFMRETEYRSADFVILTMAVAI
                     LFAFAVAIVNWALHKVTGFRFLSLMAQQVTFVMVPPLFLVFLVLGTIFIGVATPTEGG
                     AMGAVGAMLLALGKRITDRNPKRFNFAIVKSATEATAKLSAFVMFILIGARIFSLTFY
                     GVNGHVWVEHLLTSLPGGEIGFLIFINVLVFVLAFFLDFFELSFIIIPLLVPAAETLG
                     IDLIWLGVMLSVNMQTSFMHPPFGFALFFLRSVAPREAFIDKITGKLTDPVTTGQIYW
                     GAVPFVFIQVIMIGLVIAFPSMVTHYRGAAPTIDPSKIEIQLPSFDSGPSGFGAPPSF
                     GPPGGPAFPSAPPVFGAPPAFETPSTPAPPAQKEEPAGGGGN"
     gene            complement(4177..>4487)
                     /locus_tag="VF226_09990"
     CDS             complement(4177..>4487)
                     /locus_tag="VF226_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074358.1"
                     /translation="MIKAAAHAANTYTMARYDAMNAAALKRLVANGTQLRPFSEAILD
                     ACFKATNETYDEIAAKNADFKKIYEAMKEVRGDGYLWFQITEHTFDSYMMIQQRKRAL
                     "
BASE COUNT          695 a         1446 c         1378 g          968 t
ORIGIN      
        1 gtgtcgtgac catcgtcgaa cacgatccgc acggcgtagt tgccgaccgg ccgcagctcc
       61 ctgatcttga cgttccgctt gcgtgcgacc gtcacccgct gatccgggct gtggccttgc
      121 acctcggcgg acggactcat gacgcggagc atttcggcag acagctcgta gctctcgccg
      181 tcgtcgaagg tgacggccag ggcgtcccgg ttcgggctga tctcgagctt cggcggcttg
      241 acgcgagtgc cctccgcggc ctgaacctcc gcccaggacc tggcggcgat ctcgcgatag
      301 atctgagcgt gcgggctgtc cggattggtg gcgacgatcg gctgtccggc atccgaccgt
      361 gacctgatct ccatgtgcaa cggcacggcg ccgaggaagg gaacgccgag cttgtctgcc
      421 tcgatctccg cgccgccgga gccgaatatg tcagagcgtt cgccgcattt cgggcagatg
      481 aagtagctca tgttctcgat gatgccgagg acgggcacgt ccaccttgcg gaacatgttg
      541 aggcccttcc gggcgtcgat cagagcgagg tcctggggtg tcgagacgat cacggcgccc
      601 gacagcggca cctgctgggc catggtgagc tgcacgtcac ccgtgcccgg cggcatgtcg
      661 atgaccatga cgtcgagatc gtcccaggcg acctcgcgca gcatctggtt gagggccgag
      721 acgaccatcg gcccgcgcca gatgatcggc gtcccctcat cgaccaggaa gcccatcgac
      781 atcacctgca ggccatagcc ctgcatcggc atcatcgttt ttcccgccac cacgtgcggc
      841 cgcccggtca ggcccagcag ccgtggctgg gacgggccat agatatcggc gtccagaatt
      901 ccggccttga ggccgatggc gcggaacccg agcgcgagat tgacggcggt tgtcgacttg
      961 ccgacgccgc ccttgcccga agcgacggca atcatatgct tgacgccggg aacgcccgcc
     1021 acctgccggc cgccgcccgc cctcgcccgt ggcgcaccgg ccccggcctg ggccgctgcc
     1081 cgggcctgct gcacgcgcgg atgctccact acgttgcggc tgccgttcgc gccggcggga
     1141 ggcgtggcgc tgggcccctt ggtttcggcc gtcagcacgg cggtcacgcc ggacacgccg
     1201 gggacctcct tcaccacctt ctcggccgct tggcgcaacg gctcgagctc ctgggctctc
     1261 tcgagcggta cggtgatcga gaaatagacg cgcccgttct tgatgaggat ttcagaaacg
     1321 agacccagat cgacgatatt gctttcgaga tccggtccct ttacacgccg gagctgctcc
     1381 agcacctgat ccttcgttac ggacatgctc tttctccttc gacgctggtc gcgggcgtcg
     1441 attcattccc cgggggccaa ttcgcttacg tccatcgtcc acgcggcagt tgtgtcgctg
     1501 gaagtcgctc gatgcccccg ctatacacgc ctcagcgagg gcgcagcacg gtatgaacta
     1561 tatctgtcgg aggtgcgaat gatgaaggga ctcctggcgt tcagtcgcac cgtcgaccgc
     1621 gcgacagggt gggtcggcaa atcggtggct tggctcatcg tcgctgccat tctcatcagc
     1681 gccggcaacg ctagcgtccg caagctcttc gacaccagct cgaacgcctg gctcgaactg
     1741 cagtggtggc tgttcggggc ggtattcctg ctctgcgccg cctggacgct ttcctcaaac
     1801 gagcatatcc gcatcgatat cgtcaacaat catctctccc gatcggcccg gaacatcatc
     1861 gagcttgtcg gccacctgct gttcctcatg cccatgtgcc tggtgatcct ctacacctcc
     1921 attccattct tcctgcgttc ctgggagcag aatgaacaat catccaatgc gggcggtctc
     1981 ccgctctacc cgcccaagct gctcattgcg ctcggtttcg ctttgctgtt gctgcaagcc
     2041 atttccgagc tgataaagcg catcgcgatc atgcgcggag atttggagga agaaggttcc
     2101 tcgcctggcg gtcacaaagc ttccgccgag gccgaagcga agcggcttct cgaggacggt
     2161 gcgcagtcgg gctagccgat acggcatgct gccactggcc taaagggcgg cggggggctt
     2221 gttgcggcgt ctagactcgc cggttcagaa gtattttcaa gggcgcccac gggagatttt
     2281 tagatggccg cattcatagc agagaacatt gcgccgatca tgttcgtggc gctcgtcgtc
     2341 gttctgctgc tcggctatcc agttgccttc tcgctgggcg cagttggcct catatttttc
     2401 gtcatcggcg tcgagctcgc gccgctttcc gacggcgcca tcaacctctc ctggcccctg
     2461 ctgcagtcac tgcccgaacg catattcggc atcgtgaaca acgacacgct gctggcggtg
     2521 ccgttcttca cttttatggg gctcatcctc gaacgctccg gcatggccga ggatctgctc
     2581 gacaccatcg gtcagctctt cggcaccgtg cggggtggcc tggctttcgc tgtgattttc
     2641 gtcggcgcgc tgctcgcggc gacgacaggc gtggtggccg catccgtgat ctcgatgggg
     2701 ctcatctccc tgcccatcat gctgcgctac ggttatgaca ggcgggtcgc atccggcgtc
     2761 atcgcggcct cgggaacgct cgcccagatc attccgcctt cgctcgtgct catcgtgatg
     2821 gcggatcagc tcggtcgatc ggtgggcgac atgtaccagg gagcgttcgt tcccggcctg
     2881 gtgctggcag gcctgtacgc cgcttatgtg gggctggtga gcctgatctg gccgaaagca
     2941 acgccagggc tgccgccgga ggcgatcggc ttcaaggaaa agaacggttc gcgcggattg
     3001 acatcgctgg cgatcctcac cgtcgtatcg tccgtgttcg gttggtactt catgagggaa
     3061 acggagtacc gcagcgccga cttcgtcatc ctgacgatgg ccgtggcgat cctgttcgcg
     3121 ttcgccgtgg ccatcgtgaa ctgggccctc cacaaggtga cgggctttcg atttttgtcc
     3181 ctcatggccc agcaggtcac cttcgtcatg gtgccgccgc tgttcctcgt gtttctcgtg
     3241 ctcggcacca tcttcatcgg cgtcgcgacc ccgaccgagg gcggtgcaat gggtgccgtg
     3301 ggcgccatgt tgctcgcgct cggcaagcgg atcacggatc gcaacccgaa gcggttcaat
     3361 ttcgcgatcg tgaagtctgc gaccgaagcg accgcgaagc tgtcggcttt cgtgatgttc
     3421 atcctgatcg gtgctcgtat tttctcgctg accttttacg gcgtgaatgg gcacgtctgg
     3481 gtcgagcacc tgctcacctc tctgccgggt ggggagatcg gcttcctcat cttcatcaac
     3541 gtgctggtct tcgtgctggc cttctttctc gatttcttcg agctctcgtt catcatcatc
     3601 ccgctgctcg tgcctgcagc ggagacgctc ggcatcgatc tcatctggct gggtgtcatg
     3661 ctgagcgtga acatgcagac gagcttcatg catccgccct tcgggttcgc gctgttcttc
     3721 ctgcgctcgg ttgcgccgcg cgaagccttc atcgacaaga tcaccggcaa gctcaccgat
     3781 cccgttacga cgggccagat ttattggggc gcggtaccgt tcgtattcat ccaggtgatc
     3841 atgattgggc tggtgatcgc attcccgagc atggtgacgc attaccgggg ggcggcgccc
     3901 acgatcgatc caagcaagat cgagattcag cttccgagct tcgactctgg cccgagcggg
     3961 tttggcgcgc ccccgtcgtt cgggcctccg ggcggaccgg ctttcccgag cgcgccacct
     4021 gtgttcggcg cgccgcctgc cttcgagacg ccgagcacac ctgcgcctcc tgcgcaaaaa
     4081 gaggagccgg ctggcggcgg cggaaactga gcgcacgaaa aagcccggcg cgttttcgcc
     4141 gggctttcct tttctggtct cgtctgagct ttcggatcag agcgcacgct tgcgctgctg
     4201 gatcatcatg tagctgtcga aggtgtgctc cgtgatctgg aaccacagat agccgtcacc
     4261 gcggacctcc ttcatggcct cataaatctt cttgaagtcc gcgttcttcg cagcgatttc
     4321 gtcgtaggtc tcattcgtcg ccttgaagca agcgtcgaga atggcttctg agaacggccg
     4381 cagctgtgtc ccgttcgcca ccagccgctt cagggcagct gcgttcatgg cgtcatagcg
     4441 ggccatggtg taggtatttg ccgcatgggc cgcggccttg atcatgg
//
LOCUS       DASVDU010000157         8067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_9478, whole genome shotgun sequence.
ACCESSION   DASVDU010000157 DASVDU010000000
VERSION     DASVDU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8067
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9478"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            504..1247
                     /locus_tag="VF226_09995"
     CDS             504..1247
                     /locus_tag="VF226_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7074359.1"
                     /translation="MKLHILSDLHTEFADFVPPETDAEAVILAGDIGVGVEGMRWAAR
                     HFPVRPVIYVPGNHEFYGHDIDLTAELDAAAPDNITLLSDDVLVLDGVRFLGSTLWTD
                     FKLHGEGEAWFARERARQSMNDFFRIRQGGHTFRPEDSVVLHEASRAWLERQLEQAFD
                     GPTVVITHHLPALKSVAPRYANDALNPAFASRLEGLIDEYQPALWIHGHTHDACDYEL
                     FRTRVVCNPRGYPGEYGHAGFKPDLTVTV"
     gene            1320..2123
                     /locus_tag="VF226_10000"
     CDS             1320..2123
                     /locus_tag="VF226_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7074360.1"
                     /translation="MFICAAGDIHGAMDRLYEDVLAFEESLGVRFNYVLHVGDFGIWP
                     DPDRVDKATRNHDGAGDFPVWFAENRAAPRATVFIKGNHEDFVWLDEQLKAGRRAILP
                     GLTYLPSGDVIDIGEDRERIRIGGIGGCHGPSNYERRSRDLQGLAKRHFTQDECERMS
                     SHGGVDVLLLHDAPAGVEFTWRRKDGSVRRRYRSEAEGLAQAVARARPRVCFFGHHHT
                     RLDSEIAGVRCIGLNKVAMPGNLVALDIPARGHGYEILGEWPRREQLPS"
     gene            2180..2488
                     /locus_tag="VF226_10005"
     CDS             2180..2488
                     /locus_tag="VF226_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7074361.1"
                     /translation="MISEIEQHRGEIEALCREYGVERLALFGSAATGGLRANSSDLDF
                     LVEFRTPLASGYANRYFGLLEGLQSLFKRPVDLVVDSAIRNPYFREAVDRNKALLYAA
                     "
     gene            2478..2846
                     /locus_tag="VF226_10010"
     CDS             2478..2846
                     /locus_tag="VF226_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEX7074362.1"
                     /translation="MRLESRKYLYDIKQAADLLGEFTAGQQFTDYEENSMLRAAVERK
                     FEIIGEALSKLSKLDSGITSGIGEHARIIAFRNILIHGYAEIDDRLVWDIVENKLPVL
                     RRESTNCWQKIDSRKSGRPK"
     gene            complement(3169..4383)
                     /locus_tag="VF226_10015"
     CDS             complement(3169..4383)
                     /locus_tag="VF226_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX7074363.1"
                     /translation="MDRHGVATGRFIYGKSYLARENAVPIDPIALKLSMKTYETRLLK
                     GVFGALRDASPDHWGRRIIERHFGSPGPDELDYLLHSPDDRAGALSFGLNREPPAPKR
                     EFNRTMALEKLQAFADAIIRDEELPQDADAQQAQDLMNGGTSMGGARPKAVVEDDGAL
                     WVAKFNREDDKWNHARVERAMLMLARECGIRTAETRVTTIGDRDALLVKRFDRERTER
                     GYQRARMLSALTLLRAEDTHQDRSKWSYVLLAEELRRVSVQPKTDAPELFRRMCFNAL
                     ISNTDDHPRNHAVIAMRTDWKLSPAYDLVPNMPISLERRDLALECGDMGRYAHADNLL
                     SQCARFLLKHDEAKEIVDTMERTVKDRWHDIARREGVTEKDCETISPAFAYEGFRLTL
                     AEQRTSQLIENI"
     gene            complement(4454..4795)
                     /locus_tag="VF226_10020"
     CDS             complement(4454..4795)
                     /locus_tag="VF226_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7074364.1"
                     /translation="MARKNTLTEAPPYAVEQAIRRLGANLRTARLRRNLTIGEVAEKI
                     GTGPRAVSDAEQGKITTGIVVYTALLWAYDLLEPIEILADPSRDPEGLSLSSQRERAR
                     AQKPGELDSDF"
     gene            5384..5731
                     /locus_tag="VF226_10025"
     CDS             5384..5731
                     /locus_tag="VF226_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074365.1"
                     /translation="MATQATLEVLFGGKAAAKVLLFIENYGDGYASRIAKTFDMPLSE
                     VQKQLQKFEQVGILVSRMVGTSRIYTWNPRDPALGGLRELLRQTLERGIPEKTLQLYF
                     RQRQRPRRQGKAI"
     gene            5732..6319
                     /locus_tag="VF226_10030"
     CDS             5732..6319
                     /locus_tag="VF226_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074366.1"
                     /translation="MPEIIEQSTVEEVAAIVSDALENAGITATLSGGGAVTIYSNNAY
                     LSRDLDFVTSVMVADLEPVLKTLGFENTRVPRLSQFSHPKVKWYVEFPPSPLMFGHLY
                     VSPADCAVLELPVGRLRIITPTQSVMDRLAAAYAWKDAQSRDQAIMVAANQDIDWEVL
                     KAWFADEGESEQEYRRFADAVRERKRRKGTQRTFR"
     gene            complement(6447..6737)
                     /locus_tag="VF226_10035"
     CDS             complement(6447..6737)
                     /locus_tag="VF226_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074367.1"
                     /translation="MRSGDCEVNLVAPGLAAGRPRRPRSTKSNFAFCGRICLVAAKST
                     SASSSPGGVREVDDQVWQVSFLGYDLGYFDTDKDRVEQIPTRSPRRKFGRPE"
     gene            6800..>8067
                     /locus_tag="VF226_10040"
     CDS             6800..>8067
                     /locus_tag="VF226_10040"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7074368.1"
                     /translation="MLLAATPAVADPGAISIEVVDAVTRRPVDGAKITAESRDGDVRS
                     ATTDKGRALLDSLEAGFFEFRAESAGYVTAVEPAVRVLEQRTRRLRFELQPVSGPMDE
                     IVVVGSAREADPFGAVSSTFLSRDELRNAPGSGSDVMRALSGLPGVVSNGEFAAFSVR
                     GHGPKSNLIYVDGFPFQQVVHFEQTLGEEEEIINGGRYSIFAPNAVAGAEFSPGGWSA
                     EFGGRDASLLQLEVVDGAPSPVGSLRLDLAGAELLYEGPSGFHDDTSMFLQARRFDFG
                     QFFEIIGEESLGAPVSTDVILKTHTRLDDDDEFEFLAIYAPEEYTRDVNHILAAEEEG
                     EAIEDVTLQNEDQDLALVGGTWRRLFGDDGEWTNRIYYRERDRVSSEGEAFPDLVPPG
                     TPADQVPVRERPLTVNEKESELGWRSDVSFG"
BASE COUNT         1532 a         2390 c         2475 g         1670 t
ORIGIN      
        1 ttcgcgtgac ccctttcagc tcacgttgat tcgccgcaga accaccaact cgggcgttac
       61 cgtttcttac atacgctgtt ggcggcatcg atcagcttcg gtaactccgc cgccgaatag
      121 tgtgtcgtaa ttccgcgaat cgtcaaagta gggcttgccc gcctatcatc cctttccttg
      181 ggcaaaggga aaggacgtgc tgtcctttcc ttgtggaaaa ggaaacttca aatatgttct
      241 ggccagcacc ctgatcgcac gtggcccgca agctggagac agccgtgctt ctgcattggc
      301 gtcgtcagcg acaggatctt ggctatgtcg ccggcgagcg ggagatcgta acctcgtcgt
      361 gaaccccgag cggccccagg tcttttgacc ggaaacctgc ctgtgccaac acaagagctc
      421 ttgcggcaac aattccccga ctgacgcttg caatgcgacg tttgccgggt gttacgcttc
      481 ggcacgacgc ggcgcgcact actatgaaac tccacatcct gagcgatctg cacaccgaat
      541 tcgcggactt cgtgccaccc gaaacggatg ccgaagcggt gattcttgca ggcgacattg
      601 gtgtgggcgt ggagggtatg cgctgggcgg cgcgccactt tcccgttagg cctgtcatct
      661 acgttcccgg caatcacgag ttctacggcc atgacatcga cctgaccgcg gagctcgacg
      721 cggcagcgcc ggacaacatc actctattga gtgacgatgt tctggttctg gacggcgtcc
      781 gcttccttgg cagcacgctt tggactgact tcaagctgca cggggaaggt gaagcctggt
      841 ttgcccgcga acgcgccagg cagtccatga acgacttctt ccgcatcaga cagggcgggc
      901 acacgtttcg gccggaggat tcggtcgtgc ttcacgaggc gagccgggca tggctcgagc
      961 gccagctcga gcaggctttc gacggcccga ccgttgttat cacgcatcat ctgcctgcgt
     1021 tgaagtccgt tgcgccgcgc tacgcgaacg atgccctgaa cccggcattc gcgagccgcc
     1081 tcgaaggcct gattgacgag tatcagccgg cactttggat tcacggccat acccacgatg
     1141 cctgtgatta tgagctgttc cgaacccggg tcgtctgcaa tccgcggggg tatccgggag
     1201 agtacggcca tgccggcttc aaacctgatc tcaccgtgac cgtctaacgt acacgtcgtc
     1261 gggtagactt ttgcgcttca accccgacgc ggattcatag gaagcacgac gaggaagtca
     1321 tgttcatctg cgccgccggc gacatccatg gagccatgga tcgtctctac gaggacgtcc
     1381 tcgccttcga ggagtcgctg ggcgtgcgct ttaattacgt gcttcacgtc ggcgattttg
     1441 gcatctggcc cgatcccgac cgggtcgaca aggcgacgcg taatcacgac ggcgcggggg
     1501 atttcccggt ctggttcgcc gagaatcgtg cagctcctcg cgccacggtt ttcataaagg
     1561 ggaatcacga agacttcgtc tggctcgacg agcagctcaa ggccggtcgg cgcgcgattc
     1621 taccgggcct gacatatctg ccgagtggcg acgtcatcga tatcggcgaa gaccgcgagc
     1681 ggatccgcat tggcggtatc ggcgggtgtc acggtccatc caactacgag cgtcgctctc
     1741 gtgatctaca gggactggcc aagcgtcact tcacgcagga cgagtgcgag cgcatgagca
     1801 gccacggcgg cgtcgacgtg ctcctccttc atgatgctcc cgccggggtc gagttcactt
     1861 ggagacgcaa ggacggctcc gtccgtcgcc ggtaccggag cgaggcggaa gggctcgccc
     1921 aggcggttgc ccgcgcccgc ccgcgggtct gctttttcgg ccatcaccac actcggctcg
     1981 actcggagat tgccggcgtt cgttgcatcg gcctcaacaa ggtggcgatg ccgggtaacc
     2041 tcgtggcgct cgacataccg gcgcgaggcc acggttacga gatcctgggc gaatggccgc
     2101 gtcgcgagca gctcccttcc taacgtgacg tccgccgtgg catggcattc tgggttaaaa
     2161 tggcacgcgg agtacgacca tgatttcgga aatcgaacaa caccgcgggg aaattgaagc
     2221 gctgtgtcgt gagtacggcg ttgagcggtt ggcgctgttt ggctcggctg caactggcgg
     2281 ccttcgagcg aacagtagcg acctggattt cctcgtagag ttccggaccc cgctggcctc
     2341 aggctatgcc aacaggtatt tcggactgct tgaaggcctg cagagtcttt tcaagcggcc
     2401 ggtggatctg gttgtcgact ctgccatcag gaatccctac ttccgcgaag cggtagaccg
     2461 gaacaaggcg ctgctatatg cggcttgagt cccggaagta cctctacgac atcaagcaag
     2521 ctgctgacct gctcggggag tttaccgccg ggcagcagtt tacggattac gaggagaact
     2581 caatgctgcg cgccgccgtg gagcgtaagt tcgagatcat cggcgaggcg ctttcaaagc
     2641 tttccaagct cgactcgggg ataacgtccg gcatcggcga gcacgcgaga atcatcgctt
     2701 ttcgaaacat tctcatccat gggtacgcgg aaattgacga ccggctcgtc tgggatatcg
     2761 tggagaacaa gctaccggtg ctacggcgcg agtcgacgaa ctgctggcaa aagattgact
     2821 cgcgaaagag cggccgcccg aaataaatcg tcacattaga agcaggtggg cgaaagccgt
     2881 gcgtgagatt tgcgtcattg agctgtgcgc ggtcatgcat cgtactgcaa attgagcaac
     2941 ggacgccccg gtaactggtt gaaaacatgg ccacgaggct ttcacccgaa agctgaaaat
     3001 ccgcgtgtcg gtggttcgat tccgcccctg gccaccaatc tttcagggtg tattccggtc
     3061 acatggtcta caccttattc cggcggtgag catcatgatt gttgtcgcgt cacctggtcg
     3121 ctatcgattc acaagagcgg cattcatcta caacggccac aggtgcgctc aaatattctc
     3181 gatcaattgc gaggttctct gctcggccag cgtcagccga aacccttcat aagcgaaggc
     3241 cgggctgatg gtctcgcagt ccttttcggt cacgccttcc ctgcgggcga tgtcgtgcca
     3301 gcggtctttg actgttcgct ccatcgtatc gacgatttct ttggcctcat cgtgtttgag
     3361 cagaaagcgg gcgcattgtg aaagaagatt gtcggcatgg gcgtagcgcc ccatgtcgcc
     3421 gcattcaagc gcgagatcgc ggcgctcgag gctaatcggc atgttgggaa cgaggtcata
     3481 ggccggagag agcttccagt cggtccgcat cgcgatcacc gcatgattgc gcggatggtc
     3541 gtccgtattg ctgatgagcg cgttaaagca catgcgccgg aacagttcgg gcgcatcggt
     3601 cttcggctgc actgaaaccc ggcgcagttc ttcggcgaga agcacatagg accatttgct
     3661 gcggtcctga tgcgtgtctt cggcgcggag cagtgtgaga gcgcttagca tgcgcgccct
     3721 ttgatagccg cgttccgttc gttcccggtc gaagcgcttt accagcagcg cgtcgcgatc
     3781 gccgatcgtc gtgactctgg tttctgcagt ccggatgccg cattcgcggg cgagcatcag
     3841 catcgcgcgc tcgacccgtg cgtgattcca tttgtcatcc tcgcgattga acttcgcgac
     3901 ccagagcgcg ccgtcatcct cgacaaccgc cttgggccgg gcaccgccca tcgacgtgcc
     3961 gccattcatc aggtcctgcg cctgctgtgc gtccgcgtcc tgcggcagtt cctcgtctct
     4021 gatgattgca tcggcgaagg cctggagctt ttcaagcgcc atagtgcggt tgaattctcg
     4081 tttcggggcg ggcggttcac ggttcaggcc aaagctgagc gcgccggcgc ggtcatcggg
     4141 cgaatgcagc aggtagtcga gttcatcggg ccccggtgag ccgaaatggc gctctatgat
     4201 gcggcggccc caatggtcgg ggcttgcgtc gcgcagcgcg ccgaaaacgc ccttgagaag
     4261 tctcgtctcg taagtcttca tcgaaagctt gagcgcgatc gggtctatcg gcacggcgtt
     4321 ctcgcgtgcg agatagctct tgccgtagat gaaacggccc gtggcaacgc cgtgccggtc
     4381 cacgctgcgc tcaaagcgcc ctgccgtgac gaattccgtt tcgcccggca ggacgatata
     4441 gacgaagcat tccctagaaa tcactgtcga gctccccggg tttttgcgcg cgggcgcgct
     4501 cgcgctggct ggacagggag aggccttcag gatctcttga cgggtcggcc aggatctcta
     4561 ttggctcaag caggtcgtaa gcccacagca gcgccgtgta gacaacgatg cctgtcgtga
     4621 tcttcccctg ctcggcgtcg gatacggccc gggggccggt cccgatcttc tccgccacct
     4681 ccccgatggt cagattgcgg cgcagccggg ccgtacgcag gttcgcgccc aggcgcctga
     4741 tcgcctgttc gaccgcgtag ggcggggcct ctgtgagtgt attcttcctt gccatgcgcc
     4801 ttttaaagct gcttaccgct gctgatctgc tttttaaagc atatcaacga ttcgcgaccg
     4861 cgccaagcat aatctgcttt ttaaggcaga ttaaccccaa taggaagctt tttaaagcag
     4921 tcgagaaccg gaacatcgag cccggctcag gccgggcgaa atgagctccg gctcagcggc
     4981 cggggttgag gagggatgga atgcctgatg tattcgactc cgacgattcc ctctcaacct
     5041 gcccgagtcc cgccagacaa tgcttggagg gtggattctt ggcgtctgct gagttgcaag
     5101 gcgccaaact cgcgaggctg acgaatgcgc gatatcgcct atcgttttcg agacaccaat
     5161 ccatgctgga gtggctcggc ggctcgtacg atccagacgg cttcctggcg gggaccgtca
     5221 aattcgacga cccgcgggcg cggtggcgga tcgcctttct tgactgtaac tgacactggc
     5281 ttttcgactt ttgcacggtg tgcgcgtggg cctttactta cctatgtggt aggcatattc
     5341 tacctttcaa ataggtgtat atgccctacc tggtaggtgg aagatggcga cccaggcgac
     5401 actggaggtg ttgttcggcg gcaaagcggc tgcgaaagtg ctgctattca tcgagaacta
     5461 tggcgacggc tacgcctcgc ggatcgcgaa gaccttcgac atgccattaa gcgaggtgca
     5521 gaagcagctg caaaagttcg agcaggttgg aattctggtc agccggatgg tcggtaccag
     5581 ccgaatctac acctggaatc cccgggatcc ggctttgggc ggcctgcgag agctgttgcg
     5641 acaaacgctg gaacgaggga ttcccgaaaa gacactccaa ctgtacttcc ggcaacggca
     5701 acgcccgagg cgccaaggca aggcgatttg aatgccggaa atcatcgaac agtcgaccgt
     5761 cgaagaggtg gcagcaatcg tttcggacgc cctcgaaaat gccggcataa cagcgacgct
     5821 ttccggcggc ggcgctgtga cgatctattc gaacaacgcc tacctatccc gggatctcga
     5881 cttcgtgact tcagtcatgg ttgccgatct cgagcccgta ctgaagaccc tcggtttcga
     5941 aaacacgcgc gttccaaggc tgtcgcagtt cagccatccg aaggtcaagt ggtatgtcga
     6001 gttcccgccg tcaccgctaa tgttcggaca tttgtacgtg agtcccgcag attgcgcagt
     6061 gctcgaactt cccgtcggca ggctccgcat cattacgccg actcaatccg tgatggacag
     6121 gctggcggca gcctacgcgt ggaaggacgc gcagtcgcgt gaccaggcga ttatggttgc
     6181 agcaaatcag gatatcgact gggaggtgct gaaggcctgg ttcgccgacg aaggagagtc
     6241 agagcaggag taccgtcgtt tcgcagacgc tgtccgtgaa cgcaagaggc gcaaaggcac
     6301 gcaacggacg ttccggtaaa cgatcctggt tgaaagaaat acgcagcctc aaccactcag
     6361 gcggtctaaa atccgcgtgt cggtggttcg aatttgcccc tggccaccga tcaaatcaga
     6421 tgcattcttg gcacatggtt tgcctcctat tccggacggc caaactttct ccggggtgaa
     6481 cgggttggga tctgctcgac gcggtccttg tccgtgtcga agtagcccag gtcatacccg
     6541 aggaagctga cctgccagac ctggtcatcg acctcgcgga ccccacccgg cgacgacgat
     6601 gccgaagttg attttgcggc gacgaggcag atgcggccgc agaacgcgaa gttggacttg
     6661 gtcgaccgag gtcttcgcgg ccgccctgcc gcgagccccg gcgccacaag attgacctcg
     6721 cagtcaccac ttctcacgag gtctccccat gttacgtcgt ttccatcaag ctgttttcgg
     6781 aatacaaaag gccatttatt tgctactcgc ggcaacgccc gctgttgccg acccaggcgc
     6841 gatctcgata gaggtcgtcg atgccgtgac gcgccggccc gtcgatggtg cgaagattac
     6901 ggccgaatcg cgtgacggcg atgtgcgctc ggcaacgacc gataaaggcc gggcgctgct
     6961 cgacagcctg gaggccggtt tctttgaatt tcgcgccgaa tcggcgggat acgtcacggc
     7021 cgtcgagccc gcggtccgag tactggaaca acggacacgc cgtctccgct tcgaactgca
     7081 gccggtatcc gggccgatgg acgagatcgt cgttgtcggc agtgcacgcg aggcagaccc
     7141 cttcggcgcc gtctcgagca cattcctgag ccgcgacgag ctgcgtaacg cgccgggcag
     7201 tggcagcgac gtcatgcgcg cgctgagcgg tctgccaggg gtcgtctcca acggcgagtt
     7261 tgcggccttt tccgtgcggg gccatggccc caaaagcaac ctcatctacg tcgatggttt
     7321 cccgttccag caagtcgtgc acttcgagca gacgctgggc gaggaggaag agatcatcaa
     7381 tggcggccgc tactccatct ttgcacccaa tgccgttgcc ggagcggaat tctcgcctgg
     7441 cggctggagc gcggaattcg gcggccgcga cgcgtcgctc ctgcaactcg aggtggtgga
     7501 tggcgcgccg tcgcctgtcg gcagcctgcg tctcgatctc gccggcgctg aactccttta
     7561 cgagggaccg agcggcttcc acgacgacac gtcgatgttc ctgcaggcgc ggcgcttcga
     7621 cttcggccag tttttcgaga ttattggtga agagtctttg ggtgcgccgg tttccacgga
     7681 cgtgatcctg aagacgcata cacgtctcga tgatgacgac gagttcgagt ttctcgccat
     7741 atacgcgcct gaggagtaca cgcgggacgt taaccacatc ctcgctgccg aggaggaggg
     7801 cgaggccatc gaggacgtca cgttgcagaa cgaagaccag gacctcgcgc tcgtcggtgg
     7861 cacctggcgg aggttgtttg gcgatgatgg cgaatggaca aaccgtattt actaccgtga
     7921 gcgcgaccgc gtgtcgtctg agggcgaggc cttcccggac ctggtcccgc cgggtacgcc
     7981 agccgaccag gttccggtgc gcgagagacc cctgaccgtg aacgaaaaag agtcggaact
     8041 cggctggcgc agcgatgtgt cgttcgg
//
LOCUS       DASVDU010000158        14404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1425581, whole genome shotgun sequence.
ACCESSION   DASVDU010000158 DASVDU010000000
VERSION     DASVDU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425581"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VF226_10045"
     CDS             complement(<1..1001)
                     /locus_tag="VF226_10045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7074369.1"
                     /translation="MLQKNWQDLIKPTKLNVQPGRDRDRFATLVAEPLERGFGMTLGN
                     ALRRVLLSSLQGAAVRTVQIDGVLHEFSSIPGVREDVTDIILNIKEIALKVHSEGIKR
                     MVLRKEGPGPARAGDIEASSEVEILNPDHVICHLDEGASVRMEFTADMGKGYVPAERN
                     RPEDAPIGLIPVDSLYSPVKKVSYKVENTREGQILDYDKLTMTVETDGSVRPEDAVAL
                     AARILQDQLAVFINFEEPKRHVEEERHPDLSFNAALLKKVDELELSVRSANCLKNDNI
                     VYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPNWPPDNIEELAKR"
     gene            complement(1130..1528)
                     /gene="rpsK"
                     /locus_tag="VF226_10050"
     CDS             complement(1130..1528)
                     /gene="rpsK"
                     /locus_tag="VF226_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7074370.1"
                     /translation="MAREQQARTKVRRKERKNISSGVAHVNASFNNTMITITDAQGNT
                     IAWSSAGTMGFKGSRKSTPYAAQVAAEDAGRKAIEHGMKILEVEVCGPGSGRESALRA
                     LQGIGFQVTAIRDVTPIPHNGCRPRKRRRV"
     gene            complement(1598..1969)
                     /gene="rpsM"
                     /locus_tag="VF226_10055"
     CDS             complement(1598..1969)
                     /gene="rpsM"
                     /locus_tag="VF226_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7074371.1"
                     /translation="MARIAGVNIPTQKRVVIALQYIHGIGPKFAKEICEKVGIPPERR
                     VNELTDAEVLQIRETIDRDYMVEGDLRREVAMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPAKPIAGKKKA"
     gene            complement(2175..2834)
                     /locus_tag="VF226_10060"
     CDS             complement(2175..2834)
                     /locus_tag="VF226_10060"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003578673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7074372.1"
                     /translation="MNLILLGPPGAGKGTQAAAIARTRGLVQLSTGDMLRAAVKAGTE
                     VGKQAKAIMERGDLVPDEVVIRIIDDRIAQPDCDAGFILDGFPRTLPQAAALDDLLAR
                     KGKQLDLVIELKVDDAALVERISGRYACAKCGAGYHDKFKQPKAPGVCDVCGSAEFVR
                     RADDNAETVKNRLMAYYKETSPLIGYYFAKGKLKSVDGMAPIEQVTREIERLLDDVVA
                     A"
     gene            complement(2831..4183)
                     /gene="secY"
                     /locus_tag="VF226_10065"
     CDS             complement(2831..4183)
                     /gene="secY"
                     /locus_tag="VF226_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7074373.1"
                     /translation="MASAAEQLAANLNFGAFAKAEELKKRIWFTLGALVVYRLGTFIP
                     IPGVDPVAFGQAFQSSQSGILGMFNVFAGGAVERMAIFALNIMPYISASIIMQLMSSI
                     NPTLEQLKKEGEQGRKQINQYTRYLTVVLAAFQAYGIAIGLEGTRGATGSVVIEAGWF
                     FRLTTVVTLVGGTMFLMWLGEQITARGVGNGISLIIFAGIVATLPSAIAALFELARTG
                     SLSTLMLLGLLALMLLVVVVIVFMERAQRRIPIQYPKRQVGNRMFQGDSSHLPLKLNS
                     SGVIPPIFASSLLLLPITAAQFMSGSGPEWLNTLVALLGQGQPLHLAIYVSLIIFFTF
                     FYTAVVFNPQETADNLRKYGGFILGYRPGQKTAEYIDYVLTRITVVGAIYLAAVCVLP
                     EILISRAGVPFYFGGTSLLIVVSVTMDTVAQVQSHLLAHQYEGLIKKAKLRGATRGRR
                     "
     gene            complement(4361..4906)
                     /gene="rplO"
                     /locus_tag="VF226_10070"
     CDS             complement(4361..4906)
                     /gene="rplO"
                     /locus_tag="VF226_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7074374.1"
                     /translation="MRLNELKDNPGANKLRVRVGRGIGSGVGKTGGRGVKGQKSRAGV
                     SIGGFEGGQMPLYRRLPKRGFDKWRQKDWNELSLGRLQQAIDAGRIDPAQPIDVAGLV
                     AAGVLRRPKHGVRLLGTGELKAKVEITVNHATASARAAVEKAGGKVNLIEEKVLEADE
                     EKRKKSAAKKAASGKGTKSGE"
     gene            complement(4926..5126)
                     /gene="rpmD"
                     /locus_tag="VF226_10075"
     CDS             complement(4926..5126)
                     /gene="rpmD"
                     /locus_tag="VF226_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7074375.1"
                     /translation="MANNSGRTITIEQIGSPSRRPERQEQTLIGLGLNKLHRRRTLED
                     TPAVRGMINKVQHLVRIVDEKA"
     gene            complement(5182..5760)
                     /gene="rpsE"
                     /locus_tag="VF226_10080"
     CDS             complement(5182..5760)
                     /gene="rpsE"
                     /locus_tag="VF226_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7074376.1"
                     /translation="MARFPGRDRGRDREERESEFSDKLVHINRVAKVVKGGKRFGFAA
                     LVVVGDHKGRVGFGHGKAREVPEAIRKANEAARRGLMRVPLREGRTLHHDSAGRHGSG
                     RVILRSAPPGTGIIAGGAMRAVFEMLGVQDVVAKSLGSTNPYNVVRATFDALKVQENP
                     RGVAARRGKKYGEIVARRRDGTSEAEAAPAEA"
     gene            complement(5778..6137)
                     /gene="rplR"
                     /locus_tag="VF226_10085"
     CDS             complement(5778..6137)
                     /gene="rplR"
                     /locus_tag="VF226_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7074377.1"
                     /translation="MVALTQSERRKRRVRSALRKRTKGRPRLSVHRSSKHIYAQVIDD
                     SKGHTVATASSLDKDLRGKLKTGADKAAAAEVGRLVAERAIKAGVAAVVFDRGGFKYH
                     GRVKALADAAREGGLQF"
     gene            complement(6149..6691)
                     /gene="rplF"
                     /locus_tag="VF226_10090"
     CDS             complement(6149..6691)
                     /gene="rplF"
                     /locus_tag="VF226_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7074378.1"
                     /translation="MSRIGKKPVPVPANVTATITGQTVKFKGPKGELEFTLPDKVRAS
                     QSENGIEVSPVDDSKQARAMWGMSRTTLANLVKGVTEGYSHTLEIQGVGFRAQLKGKD
                     QLQLTVGFSHDIVHKIPPGVDVAVSGARQEVITVSGIDKQLVGQVAAEIRSYRPPEPY
                     QGKGIRHQGEFVFRKEGKKK"
     gene            complement(6714..7106)
                     /gene="rpsH"
                     /locus_tag="VF226_10095"
     CDS             complement(6714..7106)
                     /gene="rpsH"
                     /locus_tag="VF226_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008934161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7074379.1"
                     /translation="MVNDPLGDMLTRIRNAQMRRQTKVVTPASNLRARVLDVLAEEGY
                     IRGYTRVERGGKAELEIELKYANGQPAIRKISRVSKPGRRVYSPVKELKTVANGLGVA
                     ILSTPKGVMPDWRAREENVGGEVLCNVF"
     gene            complement(7117..7422)
                     /gene="rpsN"
                     /locus_tag="VF226_10100"
     CDS             complement(7117..7422)
                     /gene="rpsN"
                     /locus_tag="VF226_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEX7074380.1"
                     /translation="MAKTSSTEKNNRRRKLAAQYAERRQKLKAAAKDKSKTLTERMQA
                     SMKLAELPRNSSKTRIRNRCEITGRPRGYYRKLRMCRNQLRDLASQGLIPGMVKASW"
     gene            complement(7464..8150)
                     /gene="rplE"
                     /locus_tag="VF226_10105"
     CDS             complement(7464..8150)
                     /gene="rplE"
                     /locus_tag="VF226_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7074381.1"
                     /translation="MAEKKAAKGPAGAAPKGKGGKPEKAGKADKQAQQAKPAELRSFT
                     RPEGYTPRLKAHYVNVVRDQLTKKFNYKNVMQVPRIDKIVLNMGVGEAVADRKKVENA
                     ANDLALIAGQKPVLTRARKSIATFKVREGMTLGAKVTLRGDRMYEFLDRLVTIALPRV
                     KDFRGLNPRSFDGRGNYALGLKEHIVFPEIDYDKVDEVWGMDVIVCTTAKTDDEAREL
                     LRGFNFPFRS"
     gene            complement(8155..8481)
                     /gene="rplX"
                     /locus_tag="VF226_10110"
     CDS             complement(8155..8481)
                     /gene="rplX"
                     /locus_tag="VF226_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7074382.1"
                     /translation="MPSLKIKKGDRVVVLAGRDKGKRGEVISVMPKENRALVRGVNIV
                     RRHQRQTASQEGGIISKEAPIHLSNLAIEDPADGSPTRVGFKFLEDGRKVRFAKRSGE
                     VIPERK"
     gene            complement(8481..8849)
                     /gene="rplN"
                     /locus_tag="VF226_10115"
     CDS             complement(8481..8849)
                     /gene="rplN"
                     /locus_tag="VF226_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7074383.1"
                     /translation="MIQMESNLDVADNSGARRVQCIKVLGGSKRKYASIGDTIVVTVK
                     EAIPRGRVKKGQVMRAVIVRTAKGVRRPDGSLIRFDRNAAVLINNQGEPIGTRIFGPV
                     TRELRAKNHMKIISLAPEVL"
     gene            complement(8968..9207)
                     /gene="rpsQ"
                     /locus_tag="VF226_10120"
     CDS             complement(8968..9207)
                     /gene="rpsQ"
                     /locus_tag="VF226_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004859186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7074384.1"
                     /translation="MPKRILQGVVVSDKNDKTVVVKVERRYMHPLLKKTVRRTKKYHA
                     HDEANAFKVGDVVRIQEGRPVSKHKCWHVIGSVNS"
     gene            complement(9219..9425)
                     /gene="rpmC"
                     /locus_tag="VF226_10125"
     CDS             complement(9219..9425)
                     /gene="rpmC"
                     /locus_tag="VF226_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7074385.1"
                     /translation="MKAAEIRDMTLDQLEEQLVNLKKEQFNLRFQAASGQLENTARVR
                     QVRRDIARIKTIARQKQAGQAQKG"
     gene            complement(9464..9874)
                     /gene="rplP"
                     /locus_tag="VF226_10130"
     CDS             complement(9464..9874)
                     /gene="rplP"
                     /locus_tag="VF226_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005973000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7074386.1"
                     /translation="MLQPKRTKFRKAHKGRIHGQAKGGTTLNFGSYGLKALEPERVSA
                     RQIEAARRAITRHMKRAGRVWIRVFPDVPVSKKPAEVRMGSGKGAPELWVCRVKPGRI
                     MFELDGVPDDVAREALGLGAAKLPIKTKVVTRLS"
     gene            complement(9896..10621)
                     /gene="rpsC"
                     /locus_tag="VF226_10135"
     CDS             complement(9896..10621)
                     /gene="rpsC"
                     /locus_tag="VF226_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX7074387.1"
                     /translation="MGQKVNPVGLRVGINRTWDSRWFAGRGEYGRLLHEDRAIREYIL
                     KQQRQAGISKVVIERPHKKCRVTVHTARPGVLIGKKGADIEKMRADLSKLTPSEVHLN
                     IVEVRKPEIDATLVAEGIAQQLERRVAFRRAMKRAVQSALRLGAQGIRINVSGRLGGA
                     EIARTEWYREGRVPLHTLRADVDFGYGLARTAYGVIGVKVWIYKGEIMEHDPMAAERR
                     ALDMQEGGGGGRRRESERAGAGA"
     gene            complement(10624..11022)
                     /gene="rplV"
                     /locus_tag="VF226_10140"
     CDS             complement(10624..11022)
                     /gene="rplV"
                     /locus_tag="VF226_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX7074388.1"
                     /translation="MGKPKRERSLPDNEAKAVARNLRVSPQKLNLVAGLIRGKKVNAA
                     LADLEFSRKRIATDVRKCVMSAVANAENNHGLDVDDLIVAEAYVGKNLVMKRFHARGR
                     GRSARILKPFSQITVVVREVKEEPAEAKEA"
     gene            complement(11041..11319)
                     /gene="rpsS"
                     /locus_tag="VF226_10145"
     CDS             complement(11041..11319)
                     /gene="rpsS"
                     /locus_tag="VF226_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7074389.1"
                     /translation="MARSVWKGPFVDGYLLKKAEKARASGRNEIIKIWSRRSTILPQF
                     VGLTFGVYNGHKHIPVSVNEDMIGHKFGEFAPTRTFTGHSGDRKAQRR"
     gene            complement(11341..12162)
                     /gene="rplB"
                     /locus_tag="VF226_10150"
     CDS             complement(11341..12162)
                     /gene="rplB"
                     /locus_tag="VF226_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7074390.1"
                     /translation="MALKTYRPTSHGMRNLVLVDRRELWKGAPVKMLTEGLKKTGGRN
                     NAGRITTRHIGGGHKRTYRLIDFRRRRFDQSATVERLEYDPNRSAFIALVKYEDGQLS
                     YILAPQRLAVGDTVVAGEKVDVKPGNAMPLSNMPIGTIVHNVELKPGRGGQLARSAGA
                     YVQLVGRDAGWAILKLNSGETRRVRAECMATVGAVSNPDHSNQVLSKAGRKRWLGIRP
                     TVRSIAMNPVDHPNGGRTNGGKHWATPWGKPAKGFRTRKNKATEKYIIHGRHKGK"
     gene            complement(12175..12468)
                     /locus_tag="VF226_10155"
     CDS             complement(12175..12468)
                     /locus_tag="VF226_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX7074391.1"
                     /translation="MKKLDSYDVIIAPIVTEKSTTASEHNKIVFRVPLKASKPDIKSA
                     VEQLFNVKVKAVNTIVQKGKLKQFRGRRALQSDVKKAVVTLEEGHSIDVTTGL"
     gene            complement(12465..13085)
                     /gene="rplD"
                     /locus_tag="VF226_10160"
     CDS             complement(12465..13085)
                     /gene="rplD"
                     /locus_tag="VF226_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7074392.1"
                     /translation="MQANVTTLDAGSAGSLELNDAIFGLEPRPDILHRMVRYQQLKRM
                     AGTHHAQDRSEVNVTGKKVYRQKGTGGARHGDKSVPQWRGGGKAFGPKPRSHAIDLPK
                     KVRALALRHALSAKARAGELIVLEKAESTDGKTKSLRQAFEKLELSNALIIDGAELEP
                     RFARAARNIPNIDVLPVQGINVYDILRRRKLVLTKAAVEALEARFK"
     gene            complement(13091..13798)
                     /gene="rplC"
                     /locus_tag="VF226_10165"
     CDS             complement(13091..13798)
                     /gene="rplC"
                     /locus_tag="VF226_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7074393.1"
                     /translation="MRSGLIAQKLGMTRVFTDAGEHIPVTVLKVDNCQVVAHRTSEKN
                     GYTAIQLGAGRRKPTHVTKAERQNFAIAKVEPKRKVAEFRVSPENLIDIGAELTADHF
                     IPGQLVDVTGTSTGKGFQGPMKRWNFGGLHASHGVSISHRSHGSTGQRQDPGKVFKGK
                     KMAGHLGSERVTTQNLMVVKTDPERGLLMVKGAVPGHKGGWVLVRDAVKQPVPADAPK
                     PGAFRKPAAEAAAANQE"
     gene            complement(13863..14171)
                     /gene="rpsJ"
                     /locus_tag="VF226_10170"
     CDS             complement(13863..14171)
                     /gene="rpsJ"
                     /locus_tag="VF226_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005773267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7074394.1"
                     /translation="MNGQNIRIRLKAFDHRVLDASTREIVNTAKRTGAEVRGPIPLPT
                     RKERFTVNRSPHIDKKSREQFEMRTHKRLLDIVDPTPQTVDALMKLDLAAGVDVEIKL
                     "
     gene            complement(14292..>14404)
                     /locus_tag="VF226_10175"
     CDS             complement(14292..>14404)
                     /locus_tag="VF226_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7074395.1"
                     /translation="MSVELITPIAMEEKQRFAIREGGRTVGAGVVTKIVE"
BASE COUNT         2236 a         4861 c         4358 g         2949 t
ORIGIN      
        1 aaccgcttcg ccagctcctc gatgttgtcc ggcggccagt tgggcacttc catgccgagg
       61 tgcaggccca tggaggccag cacttccttg atctcgttca gcgacttgcg gccgaagttc
      121 ggcgtgcgca gcatctccgc ctcggtcttc tggatgaggt cgccgatgta gacgatgttg
      181 tcgttcttca ggcagttcgc cgagcggacc gacagctcaa gctcgtcgac cttcttgagg
      241 agcgcagcgt tgaagctgag atccggatgc cgctcctcct cgacgtgccg cttcggctcc
      301 tcgaagttga tgaacacggc gagctggtcc tgcaggatgc gggcagcgag cgccacggca
      361 tcctcggggc gcaccgagcc atccgtctcc acggtcatgg tcagcttatc gtagtcgaga
      421 atttgccctt cacgcgtatt ctcgaccttg taggaaacct tcttgacggg gctatagagg
      481 ctgtcgacag ggatcagacc gatcggcgcg tcctcgggcc tgttacgctc ggcggggacg
      541 tagcccttgc ccatatctgc cgtgaactcc atgcggacgg aagcgccctc gtcgaggtgg
      601 cagatgacgt gatcgggatt caggatctcg acctcggagc tggcctcgat gtcccccgca
      661 cgagccggtc cggggccctc cttcctgagc accatgcgct tgatgccctc ggagtgcacc
      721 ttcagcgcaa tctccttgat attcaggatg atgtccgtga cgtcctcacg aacgccggga
      781 atcgacgaga actcgtgcag cacgccgtcg atctgaacgg tgcggaccgc cgcaccctga
      841 agggacgaca ggagcacccg gcgcagagca ttgccgagcg tcatgccgaa accgcgctcg
      901 agcggctcgg ccacgagggt cgcgaaccgg tccctgtcgc ggccgggctg gacgttgagc
      961 ttcgtcggct tgatcagatc ttgccagttc ttctggagaa tgttcgccac ggtaggcctc
     1021 actatcagcg taagcggcgc ctgcgcggag cgccgccggg gtaattgatg agcaaaatgc
     1081 agccggggcc gaagcatgct tcggccgccc aggcaatccg tgccaaccct cagacgcggc
     1141 ggcgctttct cggccggcac ccgttatgag gaataggcgt cacatcacgg atggctgtca
     1201 cctggaagcc gatgccctgc agggcccgca gcgccgattc gcgtcccgaa cctggtccgc
     1261 agacctcgac ctcgaggatc ttcatgccgt gctcgatcgc cttcctgccg gcgtcctcgg
     1321 cggcgacctg ggctgcataa ggcgtcgatt tgcgcgagcc cttgaagccc atcgttcctg
     1381 ccgacgacca ggcaatcgtg ttgccctggg cgtcggtgat ggtgatcatc gtgttgttga
     1441 agctggcatt cacgtgcgcc acgccggagc tgatgttctt gcgctccttg cggcgaactt
     1501 tcgtgcgggc ctgctgctct ctagccatgg gtgatgcggt tcctggaatc tggtctgttt
     1561 tgtcggatta tcaagcgtct gcgcgccggc cagcggatca agccttcttc ttgccggcga
     1621 tcggcttggc cgggccctta cgggtccgcg cattcgtgtg cgtacgctgg ccgcgaaccg
     1681 gcagaccgcg gcgatgacgc aggccgcgat agcagccgag atccatcagc cgcttgatgt
     1741 tcatcgccac ctcgcgccgc agatcgccct cgaccatgta gtcgcggtcg atcgtctcgc
     1801 ggatctgcag cacctcagcg tcggtgagct cgttcacccg ccgctctggc ggaataccga
     1861 ccttctcgca gatctccttc gcgaacttgg gtccgatacc gtgaatatat tggagagcga
     1921 tgacgacgcg cttctgcgtc ggaatgttaa cgccagcgat acgggccacg tgtttctcct
     1981 gcgagcatgc ggtatgacga cagatcgctc agccaccgca tcggcgagcg acatatttct
     2041 gaattttcgg cagcctaaac agcaaaaacc ggcctctggc gcttcgacac cagctccgat
     2101 ctcgccaagg tgcggcatgt aatcgcggtg cttttaccct ctcgggcccc ccgcgtcaat
     2161 gcacgcaccc cgcgtcaagc cgccacgaca tcgtccagga gacgctcgat ctcgcgcgtc
     2221 acctgctcga tgggtgccat gccgtcgacc gacttcagct tgcctttggc aaagtagtag
     2281 ccgatcagcg gcgatgtttc cttgtagtag gccatcagcc tgtttttgac cgtctcggca
     2341 ttgtcgtcgg cgcgacgcac gaactcggcc gagccgcaca cgtcgcagac gccaggcgcc
     2401 ttcggctgtt tgaacttgtc atggtagccc gccccgcatt tggcgcaggc gtaccgcccc
     2461 gaaatgcgct cgacgagggc cgcgtcgtcg accttgagct caatgacgag gtcaagctgc
     2521 ttgccctttc tcgccaacag atcgtcgagc gccgcggcct gcggcaatgt ccgggggaag
     2581 ccgtcgagga tgaagccggc gtcgcagtcg ggctgagcga tgcggtcgtc gatgatccgg
     2641 atcactacct cgtcgggaac caagtcgccg cgctccatga tggccttggc ctgcttgccg
     2701 acctcggtgc cggccttgac cgcggctcgc agcatgtctc ccgtcgagag ttggaccagt
     2761 ccgcgcgtcc tggcaatggc tgccgcctgc gtgcctttac ctgcacccgg cggacccagc
     2821 agtatcagat tcatcgccgc cccctcgtcg cgcccctgag cttagccttc ttgatgaggc
     2881 cctcgtactg gtgggccagc agatggctct gcacctgtgc gacggtgtcc atcgtcacgc
     2941 tcacgacgat cagcagcgac gtgccgccga agtagaacgg gacgcctgcg cgtgagatga
     3001 ggatctcagg caagacgcag actgcggcca gataaatcgc gccgacgacc gtgatgcggg
     3061 tcagcacgta gtcgatgtac tcggccgtct tctgtcccgg acggtaacca aggatgaagc
     3121 caccgtactt gcgaaggtta tccgccgtct cctgcggatt gaacaccacc gccgtataga
     3181 agaacgtaaa gaagatgatc agcgacacat agattgccag atgcagcggc tgtccttggc
     3241 ccagcaatgc gaccagcgtg ttcagccact cgggccccga ccccgacatg aattgggcgg
     3301 ccgtgatcgg cagcagcaac aacgatgagg cgaagatcgg cgggatgacg ccagacgagt
     3361 tcagcttcag cggcaggtgc gagctgtcgc cctggaacat gcggttgccg acctgtcgct
     3421 tcggatactg aatcggaata cgccgttgcg ctcgctccat gaaaacgatc acgacgacga
     3481 ccagcagcat cagggccagc aggccgagga gcataagggt cgacagcgaa cccgtacgtg
     3541 ccagctcgaa cagtgcggcg atcgcgctcg gcagcgtcgc cacgatgccg gcgaagatga
     3601 tgagtgatat gccgttgccg acaccgcggg cggtgatctg ctcgccgagc cacatcagga
     3661 acatcgtgcc gccgaccagc gtgacgacgg tcgtcagccg gaagaaccag cccgcctcga
     3721 tgaccacact accagtcgcc ccgcgcgtgc cttcgaggcc gatggcgatg ccgtaagcct
     3781 ggaatgcagc gagaacgacc gtcaggtagc gcgtatactg gttgatctgc ttgcggccct
     3841 gctcgccctc cttcttcagc tgctcgagcg tcggattgat cgacgacatg agctgcatga
     3901 tgatcgaggc cgagatgtac ggcatgatgt tgagcgcgaa gatcgccatg cgctcgacgg
     3961 caccgcccgc gaacacattg aacatgccga ggatgccgct ctggctcgat tggaaggcct
     4021 gcccgaatgc gacagggtcc acgcctggga tcgggatgaa cgtgccgagg cgatagacga
     4081 cgagcgcacc cagcgtgaac cagatgcgct tcttaagctc ctcggccttg gcgaatgcgc
     4141 caaagttcag atttgctgcc agttgctcgg cggcggatgc cattttcgtt caattctccc
     4201 gaagttcgac gcagccccgg cggcgatcgg tccgcatgtc cggcgggcgc ttcgcggcgg
     4261 ccgatcttcc ctgcatgggc gagcgacccg cgcttctggt caccagcata acgcggcagg
     4321 cccctcgccc gaaaggcgtt gaactccgct gcggccgggt ctattcgccg ctcttggtcc
     4381 cctttccgga tgccgctttt ttggctgctg acttcttgcg cttttcctcg tcggcttcca
     4441 ggaccttctc ctcgatcagg ttgaccttgc cgccggcctt ctcgaccgcc gcgcgggcag
     4501 aagccgtcgc gtgattgacg gtgatctcga cctttgcctt gagctcgccc gtgccgagca
     4561 ggcggacccc atgcttgggg cgacgcagca cgcccgctgc cacgaggccg gcgacgtcga
     4621 tcggctgggc cgggtcgatc ctgccggcat cgatcgcctg ctgcaggcgg ccaaggctca
     4681 gctcgttcca gtccttctgg cgccacttgt cgaagccgcg cttcggcagc cggcgataga
     4741 gcggcatctg gccgccttcg aagccgccga tgctgacgcc cgcacgcgat ttctggcctt
     4801 tgacaccacg gccgccggtc ttgccgacac cggagccgat gccgcggccg acgcggacgc
     4861 ggagcttgtt ggctcctgga ttgtccttga gttcgttaag acgcatcagt ctggctccca
     4921 gcctgtcagg ccttctcgtc gacgatgcgc acgagatgct ggaccttgtt gatcatgcca
     4981 cgcacggccg gagtgtcctc cagcgtgcgg cggcggtgaa gcttgttgag accgaggccg
     5041 atcagcgtct gctcctgccg ctccggacgg cggctcgggc tgccgatctg ctcgatcgtg
     5101 atcgtcctgc cggaattgtt cgccatgtca cgtcattccc gttcggcggt cctgcagctt
     5161 gccgcaggcg ccctgtcaat ctcaggcctc ggccggggcg gcctcggctt ccgaagttcc
     5221 gtcgcgacgg cgcgcaacga tctcgccgta tttcttgccg cgccgagccg caacgccgcg
     5281 tgggttctcc tgcaccttca gagcgtcgaa ggttgcccgg acgacgttgt agggattcgt
     5341 ggagccgagc gattttgcga ccacgtcctg cacgcccagc atctcgaaca cagcgcgcat
     5401 ggcgccgcca gcgatgatgc cggtgcctgg aggcgcagac cgcaggatta cccggccgga
     5461 tccatggcgg cctgcgctgt cgtgatgaag cgtgcggcct tcgcgcagcg gaacgcgcat
     5521 cagaccgcgc cgcgccgctt cgttggcctt gcggatcgcc tcgggcacct cgcgtgcctt
     5581 accatgcccg aagccgacgc ggcccttgtg gtcaccgacg acgacgagcg cggcgaaacc
     5641 gaagcgctta ccgcccttca ccaccttcgc gacgcggttg atgtgcacca gcttgtcgga
     5701 aaactcgctc tcgcgctcct cgcgatcgcg gccgcggtct cttcctggaa aacgtgccac
     5761 ggatccttgt cctttcgtca gaattgaagt ccgccctcgc gggcggcgtc ggcgagcgcc
     5821 ttgacacggc cgtggtactt gaagccgccg cggtcaaaca cgacggcagc cacaccagcc
     5881 tttatcgctc gctccgcgac gagccgcccg acttcggcag cggccgcctt gtcggcgccg
     5941 gtcttgagct tgcccctgag gtccttgtcg aggctcgagg cagtcgccac cgtgtggccc
     6001 ttcgagtcgt cgatgacttg cgcatagatg tgcttggaag agcggtgcac ggacaagcgc
     6061 ggccgccctt tcgtgcgttt cctcagcgcg ctgcgcacgc gccttttgcg ccgttcagac
     6121 tgtgtgagcg cgaccatgga ttccgtcctt acttcttctt gccttccttg cggaagacga
     6181 attcaccctg gtggcggatg cccttgccct ggtaaggctc aggtggcctg tagctgcgga
     6241 tctccgccgc aacctgcccg acgagctgct tgtcgatgcc ggatacggtg atcacttcct
     6301 gcctggctcc gctgaccgcc acatcgaccc ccggcgggat cttgtgcacg atgtcgtggc
     6361 tgaaccccac cgtaagctga agctggtcct tacccttgag ctgcgcgcgg aagccgacgc
     6421 cttggatctc gagcgtgtgg gagtagcctt ccgttacgcc cttgaccagg ttggccagcg
     6481 tcgtacgcga catgccccac atggcccgcg cttgcttgct atcgtcgacc gggctgacct
     6541 cgatgccgtt ctcgctctgg gaggccctga ctttatctgg cagggtgaac tcgagctcgc
     6601 ccttcgggcc tttgaacttc accgtctggc ccgtgatcgt cgccgtgacg ttcgcgggca
     6661 ccgggaccgg cttcttgccg atgcgtgaca tgacttttcc tcagctcccg cccttagaat
     6721 acgttgcaga ggacctcgcc gccgacgttc tcctcacgcg cccgccagtc gggcatgacg
     6781 cccttcggcg tggagaggat cgcgacgccg aggccgttgg ccacggtctt gagctccttg
     6841 accggcgagt agacgcggcg gccgggcttc gacacgcggg agatcttgcg aatcgccggc
     6901 tggccattcg catacttcag ctcgatctcg agctccgcct tgccgccgcg ttcgacgcgc
     6961 gtgtagccgc ggatataccc ttcctccgcc aacacgtcga ggacgcgcgc gcgcaggttg
     7021 gaagccggcg tgacgacttt cgtctgccgc cgcatctgcg cattgcggat gcgggtcagc
     7081 atgtctccga gaggatcgtt gaccattctc tcgctcctac cagctcgcct tgaccatgcc
     7141 ggggatcagc ccttgggatg caagatccct gagctgatta cggcacatcc tgagcttgcg
     7201 ataataaccc cgcgggcggc ccgtgatttc gcaacggttg cgaatccggg tcttcgatga
     7261 gttgcgcggc agctcagcca gcttcatgct ggcctgcatg cgctcggtca gcgtcttgga
     7321 tttgtccttc gcggcggcct taagcttctg gcgccgctct gcgtattgag ccgccagctt
     7381 gcgcctgcgg ttgttctttt cggtcgagct cgtctttgcc atgttttctc ctaagagcct
     7441 ttcggctgtt ctgccgaggt ccctcagcta cggaatggga aattgaagcc gcgcagcagc
     7501 tcgcgcgctt catcgtcggt cttggccgtc gtgcagacga tcacgtccat gccccacacc
     7561 tcgtcgacct tgtcgtagtc gatttcgggg aacacgatgt gctccttgag gccgagcgca
     7621 tagttcccgc ggccatcgaa gctcctcggg ttcaggccgc ggaagtcttt cacgcgcggc
     7681 agcgcgatcg tgaccagacg gtccaggaac tcatacatgc ggtcgccgcg cagtgtcacc
     7741 ttggcaccga gggtcatgcc ctcgcggacc ttgaaggtcg caatcgactt gcgcgcccga
     7801 gtaaggaccg gcttctgacc ggcaatgagc gccagatcgt tggccgcgtt ctcgaccttc
     7861 ttgcggtcgg ccacagcctc gcccacgccc atgttgagca cgatcttgtc gatgcgcggc
     7921 acctgcatca cgttcttgta gttgaacttt ttcgtgagct ggtcacgcac gacatttacg
     7981 tagtgcgctt tcagccgcgg cgtatagcct tccggacgcg taaatgagcg cagctccgcc
     8041 ggcttggcct gctgtgcctg cttgtcagcc ttgccggcct tctcgggctt gccgccctta
     8101 cccttggggg cggcccccgc cggccctttc gccgccttct tctcggccat tgttctactt
     8161 cctctccggg atcacttcgc ccgaacgctt cgcaaagcgc accttccggc catcttccag
     8221 gaacttgaac ccgacacggg tcggcgatcc gtccgccggg tcctcgattg cgaggttcga
     8281 gaggtggatc ggcgcttcct tggagatgat gccgccttcc tggctcgcgg tctggcgctg
     8341 atggcggcgt acgatgttca caccacgaac cagcgcccgg ttctccttgg gcatcacgga
     8401 gatgacctcg cccctcttgc ctttgtcgcg accggcgagc accacgaccc ggtcgccctt
     8461 cttgatcttc agcgacggca tcagagcacc tccggcgcca gcgaaatgat cttcatgtga
     8521 ttcttggcgc gcaactcgcg agtgaccggc ccgaagatac gggtgccaat cggctcgccc
     8581 tggttgttga tcagcaccgc ggcgttgcgg tcgaagcgga tcagcgatcc gtccggacgg
     8641 cgcacgccct tggcggtccg cacgattacg gctctcatga cctgcccctt cttcacgcgg
     8701 ccgcgcggaa tggcttcctt cacggtcacg acgatggtgt cgccgatgct cgcgtacttc
     8761 cgcttggagc cgcccagcac cttgatgcac tgcacacgac gggcgccgga attgtcggcc
     8821 acgtcaaggt tggattccat ctggatcatc gaaacagtcc tttcggctca cgcctgaagc
     8881 tccggcctcg tcccgtcccg cttggttgga ccgccgccgt ccgatcggct ccggcccgaa
     8941 cccgggcgca atctccggct cctgacgtca cgaattcacg cttccaatga cgtgccagca
     9001 cttgtgcttg gagaccgggc gcccttcctg gatgcgcacc acgtccccga ccttgaaggc
     9061 attcgcctcg tcgtgggcgt ggtacttctt cgtgcggcgc acggtcttct tcaagagcgg
     9121 gtgcatgtag cggcgctcga ccttaacgac gaccgtcttg tcgttcttgt ccgacacgac
     9181 cacgccctgc aggatgcgct tcggcatctc gtttctcctc aacccttctg ggcctgaccg
     9241 gcctgcttct ggcgcgctat ggtcttgatg cgcgcaatgt cgcgacgcac ctgacgcacg
     9301 cgtgccgtgt tctcgagctg tcccgaggcg gcctggaagc gcaggttgaa ctgctccttc
     9361 ttgagattga ccagctgttc ttccagctga tcaagcgtca tgtccctgat ctcggcagcc
     9421 ttcattattc ttcgacctct cgtaccccgc gcgccggctg gcttcagctc aggcgcgtca
     9481 ccactttcgt cttgatcggc agctttgcgg cacccaatcc caaagcttcc ctggcaacgt
     9541 cgtctggcac accgtcgagc tcgaacatga tgcggccggg cttcacgcgg cagacccaga
     9601 gctccggtgc gcccttgccg gatcccatgc ggacctcagc cggcttcttc gacacgggga
     9661 catccggaaa aactcgaatc cacacccggc cagcgcgctt catatggcgc gtgatggcgc
     9721 gccgcgccgc ctcgatctga cgtgcgctga cgcgctccgg ctcgagcgcc ttgagcccgt
     9781 aggagccgaa attcagcgtg gttccgcctt tggcctgacc gtgaatgcgg cccttgtgcg
     9841 ccttgcggaa tttcgtgcgt ttgggttgca gcatgatcgt tgtcctcgaa atgcgtcagg
     9901 cgccggctcc ggcgcgctca ctctcgcgcc tgcggccgcc cccgccgcct tcctgcatgt
     9961 caagggcgcg ccgctcagca gccatcggat catgctccat gatctcgccc ttgtagatcc
    10021 agacctttac gccgatgacg ccataggccg tccgcgcgag cccatatccg aaatcgacat
    10081 cggcgcgcag cgtgtgcagc ggcacccggc cttcgcgata ccactccgtg cgcgcgatct
    10141 ccgcgccacc gagccggccc gagacgttga ttcggatgcc ctgggcacca agccgcaacg
    10201 ccgactgaac cgcccgcttc atcgcacgac ggaaggccac gcggcgctcg agctgctgag
    10261 cgatgccctc ggccaccagc gtcgcatcga tctcgggctt gcgcacctcg acgatgttga
    10321 gatgcacctc cgacggggtc agcttcgaca ggtcggcgcg catcttttcg atgtccgcgc
    10381 ccttctttcc aatgagcacg ccggggcggg ccgtatgcac cgtcacccgg cacttcttgt
    10441 gcggacgctc gatgacaacc ttggaaatgc cggcctggcg ctgctgcttc aggatgtatt
    10501 cgcggattgc gcgatcctcg tgcagcaacc tgccgtactc gccgcgtccg gcgaaccagc
    10561 ggctgtccca ggtccggttg atgccgacgc ggaggccgac tggattgact ttctgaccca
    10621 tcctcaggcc tcctttgcct cggccggctc ttccttcacc tcgcgcacga cgacggtgat
    10681 ctgggagaac ggcttcagta tcctggcgct gcggccgcgt ccgcgcgcgt ggaaccgctt
    10741 catcacgaga ttcttgccca cataggcttc ggcgacgatg aggtcgtcga cgtccagccc
    10801 gtggttgttc tccgcgttgg cgaccgccga catcacgcac ttgcgcacgt cggttgcaat
    10861 gcgcttgcgc gagaactcca ggtccgccag cgctgcgttg accttcttgc cgcggatcaa
    10921 ccccgcaacg agattcagct tctgcggcga cacccgcagg ttcctcgcta ccgccttcgc
    10981 ctcgttgtcg gggaggcttc gctcacgctt gggcttgccc atcgtctcaa tcctcgccgc
    11041 ttatcttctc tgcgccttgc ggtcgccgct gtggccggtg aacgtgcgcg tcggcgcgaa
    11101 ctcgccgaac ttgtgcccga tcatgtcctc gttgacggac actggaatgt gcttgtggcc
    11161 gttgtagaca ccgaacgtca ggccgacgaa ctgcggcagg atcgtcgagc gccggctcca
    11221 gatcttgatg atctcgttac gtccggacgc gcgcgccttc tctgccttct tcagcagata
    11281 gccgtcgacg aacggacctt tccaaactga gcgtgccacg cgtggtctcc ttccgccgcc
    11341 ttacttgccc ttgtggcggc catggatgat gtacttttcg gttgccttgt tcttgcgggt
    11401 gcggaagccc ttggccggct tgccccaagg cgttgcccag tgcttgccgc cattggtgcg
    11461 gccgccgttc gggtgatcga ccgggttcat ggcgatcgag cgcaccgtcg ggcggatacc
    11521 tagccaccgc ttccggccag ccttgctcag cacttggttc gaatgatccg gattcgacac
    11581 cgcgcccacg gtcgccatgc actcggctcg tacacggcgc gtttctccgg aattgagctt
    11641 gaggatcgcc cagcccgcgt cgcgcccgac gagttgcacg taggccccag ccgaacgcgc
    11701 aagctgtccg ccgcgacccg gcttcagctc gacgttgtga acgatggtgc cgatcggcat
    11761 gttgctgagc ggcatggcgt tgccgggctt cacgtcgacc ttctcgcccg ccaccaccgt
    11821 atccccgacc gcaagccgct ggggggccag gatgtaggag agctggccat cctcgtactt
    11881 caccagcgcg atgaaggccg accggttcgg atcgtactcg agccgctcga cggtggcgga
    11941 ctggtcgaac cggcgacgcc ggaaatcgat cagccggtag gtgcgcttgt ggccgccgcc
    12001 gatgtgccgg gtcgtgatcc ggccggcgtt attgcgccca cctgttttct tcaggccctc
    12061 ggtcagcatc ttgaccggcg cgcccttcca aagctcgcgg cgatccacca gcaccagatt
    12121 gcgcatgcca tgcgacgtcg gacggtatgt cttgagtgcc atggaactgc tcctttacag
    12181 gcccgtcgtg acgtcgatcg agtggccttc ctcgagcgtc acgacggcct tcttgacgtc
    12241 gctctgcagg gcgcgccgtc cgcgaaactg cttcagcttg cccttttgga cgatcgtgtt
    12301 gaccgccttc accttgacat tgaagagctg ctcgaccgcc gacttgatgt ccggcttcga
    12361 ggcctttagg ggcaccctga agacgatctt gttgtgctcc gaggccgtcg tcgacttctc
    12421 ggtcacgatc ggagcgatga tcacgtcgta gctatcgagc tttttcattt gaaccgcgcc
    12481 tccagcgcct cgacggccgc cttcgtcagc accagcttgc gacggcgcag aatgtcgtag
    12541 acgttgatgc cctggaccgg caggacgtcg atgtttggaa tgttgcgtgc cgcccgagcg
    12601 aaacggggct ccagctcggc gccgtcgatg atcagggcat tggaaagctc gagcttctcg
    12661 aaagcctgcc gcagggactt ggtcttccca tcggtcgact cggccttctc gagcacgatc
    12721 agctccccgg ccctggcctt ggcggacagc gcgtggcgca gcgccagtgc acgcaccttc
    12781 ttcggcaagt cgatggcgtg gctgcgcggc ttcgggccga aagccttgcc gccaccgcgc
    12841 cactgcggga ccgacttgtc gccgtggcgg gcaccgccgg tgcccttctg gcggtagacc
    12901 ttcttgccgg tcacgttcac ctcggaacgg tcctgcgcgt ggtgcgtccc cgccatccgc
    12961 ttcagctgct gatagcggac catccggtgc agaatgtccg gacgcggctc gagcccgaaa
    13021 atggcatcat tgagctccag agagcctgcc gaacccgcat ccagcgtcgt cacgttagcc
    13081 tgcatgacgc tcactcctga ttggcagcgg ccgcttcggc agccggcttc ctgaaggcac
    13141 caggcttcgg cgcatcggct ggcaccggct gcttcacggc atcgcgcacc agcacccatc
    13201 cgcccttgtg gcccggaacg gcgcccttca ccatcagcaa cccacgctcc ggatcggtct
    13261 tgacgaccat caggttctgc gtggtgacac gctcgctgcc gaggtggccc gccatcttct
    13321 tgcccttgaa gaccttgccg ggatcctggc gctgaccggt cgagccgtgg ctgcgatggg
    13381 agatcgacac gccgtggctg gcatgcagac ccccgaagtt ccagcgcttc atggggccct
    13441 ggaaaccctt gcccgtgctc gtgcccgtca catcgacgag ctgccccggg atgaaatggt
    13501 ccgccgtcag ctcggccccg atatcgatca ggttttcggg cgacacgcgg aactcggcga
    13561 ccttgcgctt cggctccacc ttggcgatcg cgaagttctg acgctccgcc ttggtgacgt
    13621 gcgtcggctt gcggcggccg gcgccgagct ggatcgccgt atatccattc ttctccgacg
    13681 tgcggtgggc gacaacctgg cagttgtcga ccttcagcac ggtcacaggg atatgctcgc
    13741 ccgcgtcggt gaaaacacgg gtcattccga gcttctgtgc gatcaatccg gagcgcatca
    13801 cgtgcggtcc ttccaatacg gggtcatgca ccttcaaatc cggcgcagag gacccgggtt
    13861 ttctagagct tgatctcgac gtcgacgcct gcggcgaggt cgagcttcat cagcgcatcc
    13921 accgtctgcg gcgtcggatc gacgatatcc agcagacgct tgtgcgtgcg catctcgaac
    13981 tgctcgcggc tcttcttgtc gatgtgcggg gagcggttca ccgtgaaccg ttccttgcgc
    14041 gtcggcagcg gaatgggccc gcggacctcg gcgccggtgc gtttcgccgt attcacgatc
    14101 tcgcgcgtcg acgcatcgag cacacgatgg tcgaacgctt tgagccggat acgtatgttc
    14161 tggccgttca tgttcttcac tcaccgattg cggcgagcgc ctcctcacat cggaagcgct
    14221 ctcgcgcgcc cccacctaaa ctcttgcgtc cggtagcggc gcagcagccg ccagtgtttc
    14281 tggcccgctt ttcactccac gatcttcgtc accacgccgg cgccgaccgt gcgaccgccc
    14341 tcgcggatcg caaaccgctg cttctcctcc atcgcgatcg gcgtgatcag ctcgacgctc
    14401 atcg
//
LOCUS       DASVDU010000159         4001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_826155, whole genome shotgun sequence.
ACCESSION   DASVDU010000159 DASVDU010000000
VERSION     DASVDU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826155"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VF226_10180"
     CDS             complement(<1..960)
                     /locus_tag="VF226_10180"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7074396.1"
                     /translation="MALLATLVAGIFGFMLIGVPIGLAIIAGSIVAILLFVPGANLMI
                     VPQQVFGGLDSFTLLAIPCFLLAGSLMTMGGLTDRLLAFTNTVLGRFRGGLALSTVFA
                     ATVFAGISGSAVADTSALGRVLIPAMIREGYKPAFSAAVMASSNVLAPIIPPSIAFIV
                     MGVLTNQSITRLFLAGVIIGLVYGLAMLVITSIIAQIRQYPVHGRSTGREIWKSFRGA
                     FWALMMPVFIILGIRTGIFNVTECSAVAVVYALFVGTVIYRELTPARIYKALVETARL
                     TAIIMIVVGAARIFSWILGYAGLPQQIANAILAVTDDPWVFLIL"
     gene            complement(973..1512)
                     /locus_tag="VF226_10185"
     CDS             complement(973..1512)
                     /locus_tag="VF226_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7074397.1"
                     /translation="MIDQSAAEPELNGWQLRLDRFARMLERTLDAVLAMMLAVLVASL
                     VWQVFGRYALDSAPDWSEELARYLMVWISMLGSAVVMRENGHITVPVLVDALPTRIRT
                     VFLAVRDAVVLGTVYVLLSYGINLANLLKGQLSAAFEVSMALPYAALPAGALLIGLAV
                     VIARLTRSPYARGNPAPIA"
     gene            complement(1592..2563)
                     /locus_tag="VF226_10190"
     CDS             complement(1592..2563)
                     /locus_tag="VF226_10190"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7074398.1"
                     /translation="MRKSVWGALAGLGLVIGMATSAAAQTVTLKMAHIYTPGNIWYDV
                     AEDYAKRVAERSGGKIKIDIAHSGSTGDWPQSIEGLLIGTNDIVLQSVGTLDRYNTIA
                     GIEAYPYLIRDIAHFKSVYYGPLGRELFAEIAKKTGFHIIGAGYRGARHMSSNKLVEK
                     VDDLKGVKLRVPPLKMYRLTWEYLGASPVPMGVAELFTSLQQGVVDGQENPLEVIEDQ
                     HFDEVQKYVIETGHVLGAMTFIFSDAKFKSLPEETRKILQEEGEAAMLAGTDRMVEME
                     AKYKKDLEAKGLTFIPVNRNAFQAKLEPMAKDFPELADWVKKIQAVK"
     gene            complement(2595..3461)
                     /locus_tag="VF226_10195"
     CDS             complement(2595..3461)
                     /locus_tag="VF226_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074399.1"
                     /translation="MTRVIEAPTATKFPSEARACVVVCGSHGGRYPAYLAASAGLRAV
                     IFNDAGVGRDSAGIASLALLAAGGMAAAAVSHMSARIGEPGDMLARGVISHANEPAQR
                     LGVHPGMSCREAAERLQRAEVRRIEIPPSTETRSVLTPERAVRRLVVIDSAAMVVPED
                     ADQIIVTGSHGGLVGGNPALALRVDGHAAVYNDAGIGVEEAGIARLPALERRNIPAFT
                     VSAASARIGEALSTYEDGVISAANAPARALGALPGLQAKAVLLAWARAAGGPDRKVPA
                     SAPAPGREASSV"
     gene            3600..>4001
                     /locus_tag="VF226_10200"
     CDS             3600..>4001
                     /locus_tag="VF226_10200"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7074400.1"
                     /translation="MTQETAVKTVDRVATILRLLAQAGESGHRLSDIADKAGLGKTTA
                     HRLLGALVQVGLANQDLKTKRYTLGSAAAALGRAAGMHQVEIFIRPVLRRLARETEDT
                     VFASVREGLAAVCIGREVGAFPIRTLTLDEGD"
BASE COUNT          719 a         1249 c         1328 g          705 t
ORIGIN      
        1 caggatcagg aagacccacg ggtcgtccgt cacggcgagg atcgcattgg cgatctgctg
       61 cggcaggcca gcgtagccga ggatccagga gaagatgcgg gcggcaccaa ccacgatcat
      121 gatgatggcg gtgagccgcg ctgtttcgac aagcgccttg tagatccgcg ccggcgtcag
      181 ctcgcggtag atgacggtgc caacgaacag ggcgtagacg acggcaacgg ccgagcattc
      241 cgtcacgttg aagatgccgg tccgtatgcc gaggatgatg aagaccggca tcattagtgc
      301 ccagaaggcg ccgcggaagc tcttccagat ttcccgtccg gtcgagcggc cgtgcacggg
      361 atactgccgg atttgggcaa tgatcgaggt gatgacgagc atcgccagcc cgtagacgag
      421 gccgatgatg acgcccgcca ggaacaggcg cgtgatcgac tggttcgtca gcacgcccat
      481 gacgatgaag gcgatcgagg gcgggatgat gggggcgagg acattggagg aggccatcac
      541 ggcggccgag aatgccggct tgtagccctc tcggatcatc gccgggatga gcacccggcc
      601 gagggccgag gtgtcggcca ccgccgagcc cgagatgccg gcgaacacgg tggcggcgaa
      661 gacggtgctg agcgcgagcc cgccgcggaa acggccgagc accgtgttgg tgaaagccag
      721 cagcctgtcg gtcaggccgc ccatcgtcat gaggctgccg gcgagcagga agcatgggat
      781 ggcgagcagg gtgaagctat cgagaccgcc gaatacctgc tgcggcacga tcatcagatt
      841 ggcgccgggg acgaagagca gaattgccac gatggagccg gcgatgatgg cgaggccgat
      901 cggcacgccg atcagcatga acccgaatat tccggccacc agagtcgcaa gcagcgccat
      961 gcaagcgtcc cttcaggcaa tcggcgcggg gttgccgcgc gcgtacgggc tgcgggtcag
     1021 ccgcgcgatg acgacggcca gtccgatgag caatgcaccg gcggggagag ccgcataggg
     1081 cagggccatg ctgacctcga atgccgcgga cagctgaccc ttgagcaggt ttgcgagatt
     1141 gatgccgtag gaaagcagaa cgtacacggt gccgagcacg acggcatccc tcaccgccag
     1201 aaacacggtg cgtatccggg tgggcagggc atcaacgagc acggggacag tgatgtgtcc
     1261 gttctcgcgc atcacgacgg cgctgcccag catgctgatc caaaccatga ggtaacgggc
     1321 cagctcctcg gaccagtctg gtgcgctgtc caatgcgtat ctgccgaaga cctgccagac
     1381 gaggctcgcc acgagaaccg cgagcatcat tgcgagcacc gcatcgagcg tgcgctccag
     1441 catccgtgcg aaccggtcga gccggagttg ccagccgttc agttccggct cagccgctga
     1501 ctgatcgatc attctgggcg gggctcccaa gaaggcgacc gggcgctgat ggtcgtccgg
     1561 ccgcccgctt ttcaagctac cgggccgacc gttatttcac ggcctggatc ttcttgaccc
     1621 agtcagccag ctcggggaag tctttcgcca tcggttcgag cttggcctgg aaggcgttcc
     1681 ggttgaccgg gatgaaggtc aggccctttg cctccagatc cttcttgtat ttcgcctcca
     1741 tctcgaccat gcggtcggtg ccggcgagca tggcggcttc gccttcctcc tgcaggatct
     1801 tgcgcgtctc ctcgggcagc gatttgaact tcgcatcgga aaagatgaac gtcatggcac
     1861 cgagcacgtg gccggtttcg atgacgtact tttgcacctc gtcgaaatgc tggtcctcga
     1921 tcacctcgag cgggttctcc tggccgtcga cgacgccctg ctgcagcgag gtgaagagct
     1981 cggcaacgcc catcggcacg gggctcgcgc caaggtattc ccaggtcagc cggtacatct
     2041 tgagcggtgg cacgcggagc ttgacaccct tcagatcatc gaccttctcg acgagcttgt
     2101 tcgagctcat gtggcgcgca ccgcgatagc ccgcgccgat gatgtgaaac cccgtcttct
     2161 tggcgatctc ggcgaacagc tcgcggccga gggggccgta gtagaccgat ttgaaatggg
     2221 cgatatcgcg gatgaggtag ggataggcct cgatgccggc gatcgtattg tagcggtcga
     2281 gcgtaccgac ggactgcagc acgatatcat ttgtgccgat cagcaggcct tcgatggatt
     2341 gcggccagtc gcctgtcgag ccggaatgcg cgatgtcgat cttgatcttg ccgccggagc
     2401 gttcggccac gcgctttgcg tagtcctcgg cgacgtcgta ccagatgttc ccaggcgtgt
     2461 agatgtgcgc catcttgagg gtgacggtct gcgccgcagc cgacgtcgcc atgccgatca
     2521 cgaggccaag ccctgccaga gctccccaga ctgattttct catttcttcc ctttccttgt
     2581 ccgtgcttct cgtttcatac gctcgatgct tcccttcccg gcgcaggcgc gctcgccggc
     2641 actttccgat caggcccgcc cgcggcgcgc gcccaggcga gcaggacggc tttggcctgc
     2701 agtcccggca atgccccaag cgcacgggcc ggtgcatttg ccgcagaaat cacgccgtcc
     2761 tcgtacgtgg atagagcctc gccaatccgg gcactcgctg ccgatacggt gaaggcgggg
     2821 atattgcggc gttcgagcgc ggggaggcgg gcgatgccgg cttcctcgac gccgatcccg
     2881 gcatcattgt agacggctgc atgtccatcg acccgcagcg caagcgcggg attgccgccg
     2941 acaagccctc cgtgcgagcc ggtgacgatg atctggtccg catcctcggg cacgaccatc
     3001 gcggctgagt cgatcacaac cagtcggcgg acggcgcgct ctggcgtgag cacgcttcgc
     3061 gtctctgtgg acgggggaat ttcgatccgc cggacttccg cacgctgaag gcgttccgcc
     3121 gcctcccggc aggacatccc gggatgcacg cccaatcgtt gtgccggctc gttggcgtgg
     3181 gagatgacac cgcgcgcaag catgtcgccc ggctcgccga ttcgcgcgct catgtgcgag
     3241 acggcggccg ccgccatgcc gcccgcggcg agcagagcaa gggacgcgat acccgcactg
     3301 tcgcgtccta cgccggcatc gttgaagatg acggcgcgga gcccggcgga agcggcgaga
     3361 taggccggat accggccccc gtggctgccg cagacgacga cgcatgcgcg cgcctcggat
     3421 ggaaacttgg tggccgtcgg cgcttcgatg acgcgcgtca ttgtctgcct tggaatttcc
     3481 ccgctcgatg cccgtgattt tcgcgaagaa aaccggatga cctttgttcc atttccatgc
     3541 tatcgaagtt ccactagttt gcaaagtggt gtctcgaaaa gcgctgggcg gggaaaactt
     3601 tgacccagga aaccgcagtc aagacggttg atcgtgttgc gaccattctt cgcctgctcg
     3661 cgcaggccgg ggaaagcggg caccgcctga gcgatatcgc ggacaaggcg ggcctcggca
     3721 agacgacggc gcatcgtctc ctcggagccc ttgtccaggt cggccttgcc aaccaggacc
     3781 ttaagaccaa gcggtacacc ttgggaagtg ccgctgccgc gctcgggcgc gctgccggca
     3841 tgcatcaggt cgaaatcttt atccgtccgg tcttgcgccg gcttgcccgt gagacggagg
     3901 acacggtgtt cgcctccgtg cgcgaagggc ttgccgccgt gtgcatcggt cgcgaggtgg
     3961 gcgccttccc gatccggacg ctgacgctcg acgaggggga c
//
LOCUS       DASVDU010000160         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_837995, whole genome shotgun sequence.
ACCESSION   DASVDU010000160 DASVDU010000000
VERSION     DASVDU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837995"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1160)
                     /gene="istB"
                     /locus_tag="VF226_10205"
     CDS             complement(378..1160)
                     /gene="istB"
                     /locus_tag="VF226_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7074401.1"
                     /translation="MSAAQMERLHEHMARLRLFKSRERLEALLQKAVADEQPYADFLE
                     QVLSEEVASKTAKNITMRTNLARFPFVKGLESFDFAYQPSIDKKQIATLASCHFIEHG
                     ENVVILGPPGVGKTHLSVALGMKAIESGYRVLFTTAANLIASLMKATAEGRLDERLKF
                     YTVPRLLIIDEIGYLPIDRQGANLFFQLISRRYEKGPMILTSNQSFGAWAEVFGDRVI
                     ATAILDRVLHHAITLNIRGSSYRLKDKLKAGLVRAAETESVE"
     gene            complement(1157..2467)
                     /gene="istA"
                     /locus_tag="VF226_10210"
     CDS             complement(1157..2467)
                     /gene="istA"
                     /locus_tag="VF226_10210"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7074402.1"
                     /translation="MEQGAKPEQGIIAGDDNDAPEAMVLSKERWEEVRRLHGEEKLKV
                     SEISRRLDIDRKTVRKALKGEWKPYERGARSDGLLAEHDGFLRERAAEVNYSAQILYQ
                     ELVGERGFKGSYQTVKRFVRPLREAATVDRVCVTRFETGPGQQSQIDWGQARAYLKSQ
                     LVGLHFFVLTLGFSRRGYYRVCANEQLGLFLESHEAAFEHFGGLTREHLYDRPRTVCE
                     PVGGKWRWNETFRAFAGHWGFEPRLCAPYRAQTKGKVESGVKYLKRNFLPGRRFVDIF
                     DVQAQLDEWNATIADERIHGTTHVRPIERFVEECGALMPIAGHGSFGESRRLARIVAD
                     DYLVSFQTNRYSVPFRLIGKAVEVLAVGEAIRIEHAGRVVAEHARLAGKHQMSILPEH
                     GPGPVARNARHRYPHPAPIAELNRWIGPHEVEVRDLAIYEEAAA"
     gene            complement(2501..>2748)
                     /locus_tag="VF226_10215"
     CDS             complement(2501..>2748)
                     /locus_tag="VF226_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074403.1"
                     /translation="WRAALENINVGDMVSFSGRFFNGLDGAVLTELTKSGGAPFRVAD
                     AYAPNASGTFAKPTVTYGGLTWMPIVTGHPKIPTSGH"
BASE COUNT          449 a          950 c          830 g          519 t
ORIGIN      
        1 tcctccgccg cgccacaccg gtgggcaatc tctcgtacca ccctcgggtc caacgggaag
       61 aaccgtccga gcgtcgattt agtggtcggc gtctgcggtt caagcagcgg cgtcggggca
      121 tcgttcagat tcgccatcgc cgttacgcct tcgcgccccg tcctcccatc ctcccattgg
      181 ggcttcgtgc cctgctaggg gggcgctgcg tgtgggcctc tagccacgtc agaacacccg
      241 cctcgtccac catgacggaa cgaccgatac ggatcacaca atgacgaagc gcggcgaact
      301 ctgagcaccc tgtcaagggt cacccaattt tccccagtta gggtcatcca attttcccca
      361 cctgcgggtt gccgtcgtta ctcgacggat tcggtctccg cggccctgac cagcccggcc
      421 ttgagcttgt ctttcagccg gtaggagctg ccgcggatgt tcagcgtgat ggcgtgatgc
      481 agcacgcgat cgaggatcgc ggtggcgatc acgcgatcgc cgaagacctc ggcccaggcg
      541 ccgaagctct ggttgcttgt gaggatcatc ggtcccttct cgtagcggcg gctgatcagc
      601 tggaagaaga gattggcccc ttgtcggtcg atgggcaggt agccgatctc gtcgatgatg
      661 agcagccgcg gcaccgtgta gaacttcagc cgctcgtcga gccgcccctc cgccgtggcc
      721 ttcatgagcg aggcgatgag gttggccgcc gtggtgaaga gcacgcggta tccggactcg
      781 atcgccttca tgccgagcgc cacgctcagg tgggtctttc ccacgccggg cgggccgagg
      841 atcacgacgt tctcgccgtg ctcgatgaag tggcaggacg cgagggtcgc gatctgcttc
      901 ttgtcgatcg agggctggta agcgaagtcg aagctctcca gccccttcac gaacgggaag
      961 cgcgcgaggt tcgtgcgcat cgtgatgttc ttggcggtct tgctggcgac ctcctcactc
     1021 aggacctgct cgaggaagtc ggcgtacggt tgttcgtcgg ccaccgcctt ttgcagcagc
     1081 gcctccagcc gctcgcggct cttgaagagc cgcagccgcg ccatgtgctc gtgcagtcgt
     1141 tccatctggg cagcactcat gcagcggcct cctcatagat cgccagatcg cgcacttcga
     1201 cctcatgcgg accgatccag cggttgagct cggcaatcgg cgccggatgc gggtagcgat
     1261 gccgagcatt gcgcgctact ggcccgggcc cgtgctcggg aaggatgctc atctgatgct
     1321 tcccagccag tcgcgcgtgc tcggctacga cccggccagc gtgctcgatg cgaatcgcct
     1381 caccgacggc gagcacctcc acggccttgc cgatgagccg gaacggcacc gaataccggt
     1441 tggtctggaa gctcaccagg tagtcgtcgg caacgatgcg cgccaggcgc cggctctcac
     1501 cgaaggaacc gtgtccggcg atcggcatca acgcgccgca ctcctcgacg aagcgttcga
     1561 tcggccgcac gtgcgtagtg ccgtggatgc gctcgtcggc gatcgtggca ttccactcgt
     1621 cgagctgcgc ctgcacatcg aagatatcga cgaagcgccg gccgggcagg aagttcctct
     1681 tcaggtactt cacgcccgac tccaccttgc ccttggtctg cgcccggtac ggcgcgcaca
     1741 gccgcggctc gaagccccag tgcccagcga aggcgcggaa ggtctcgttc cagcgccact
     1801 tgccgcccac cggctcgcac accgtcctgg gccggtcgta cagatgttcg cgggtgagac
     1861 ccccgaagtg ctcgaaggcc gcctcgtgcg actccagaaa cagccccagt tgctcgttgg
     1921 cgcaaactcg gtagtaaccg cgccggctga accccagcgt cagcacaaag aagtgcaacc
     1981 ccacgagctg gctcttcaag tacgcgcgcg cctggcccca gtcgatctgg ctctgctggc
     2041 ccggcccggt ctcgaaacgc gtcacgcaca cccgatcgac cgtcgccgcc tcgcgcaacg
     2101 gcctcacgaa gcgcttgacc gtctggtagc tgcccttgaa cccacgctcg cccacgagct
     2161 cctgatagag gatttgcgcc gagtagttca cctcggcagc ccgctcgcgc aggaacccat
     2221 catgctcggc cagcagcccg tcgctcctcg ccccacgctc gtacggcttc cactccccct
     2281 tgagcgcctt gcgcaccgtc ttgcggtcga tgtcgagccg ccgcgagatc tccgagacct
     2341 tcagcttctc ctccccatgc aaccgcctca cttcctccca ccgctccttg ctcaacacca
     2401 tcgcctccgg cgcgtcgtta tcgtcgccgg cgatgatgcc ttgctcaggc ttcgcacctt
     2461 gctccatttg ccttcctctc agaaagcagg tggggaattt tcagtgaccc gaagtgggga
     2521 tttttgggtg acccgtgaca atgggcatcc acgtgaggcc gccgtaagtc acggtgggct
     2581 tagcgaacgt tccgctcgcg ttcggcgcat aggcatctgc gacgcggaac ggggcgcctc
     2641 cggacttcgt gagctcagtc aggactgcgc cgtcgagccc gttgaagaaa cggccgctga
     2701 acgagaccat gtctccgaca ttgatgtttt cgagcgcagc ccgccatg
//
LOCUS       DASVDU010000161         4958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_764084, whole genome shotgun sequence.
ACCESSION   DASVDU010000161 DASVDU010000000
VERSION     DASVDU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764084"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /gene="cas3u"
                     /locus_tag="VF226_10220"
     CDS             complement(<1..1208)
                     /gene="cas3u"
                     /locus_tag="VF226_10220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated helicase/endonuclease
                     Cas3"
                     /protein_id="HEX7074404.1"
                     /translation="MPDLTADHYDEFFRALYEAEPFPWQSRLARRVIERKAAAWPAAI
                     ALPTAAGKTACIDIAVFALACQANRASSERTAPRRIFFVVDRRIIVDEAHRRAMMLAM
                     KLRSAKRGIVKKVADRMRTLAGGDEPLACFHLRGGVYRDDAWARTPIQPTVISSTVDQ
                     IGSRLLFRGYGRSSRTWSIHAGLACNDSLILLDEAHCALPFLETLQAVASYRQWADEP
                     IRSPFHTVVLSATPPEDRTDVFRDDARDRAHPVLGARLKAEKPAALCVAARSANANAL
                     VDELVERARGLVSEDSLAIGIIVNRVATAKAVYERLKNRAQHDTVLLTGRMRPLDRDD
                     VVKEWLERLDARSAGNRRLDRPVFVVATQTLEVGANLDFDGMVSECASLDALRQRFGR
                     LNRTGRDIPA"
     gene            complement(1201..2778)
                     /gene="csb2"
                     /locus_tag="VF226_10225"
     CDS             complement(1201..2778)
                     /gene="csb2"
                     /locus_tag="VF226_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEX7074405.1"
                     /translation="MFALGIRYLNGWAMAAADGAKKEQAEWPPHPDRIFMALAAAYFE
                     TDGSVEERAALEWLETLGPPAIAVSDAEYRTVVTSYVPVNDEKVSRSRPGRATVGALK
                     DAGLAVIPEFRTRQARSFPVAIPHDPVVHLVWDDDVPSELRDSMASLSRKVTSIGHSA
                     SFVQMWVESLPRTPNLVATTKVPRFQLRISGKGRLTYLEHRFNREAVKAYADIERRVD
                     RASGKEKKMLQAELKRRFPAGAPITLRPEPGLWQGYGRPEPERRPVLPHTVFDPRLVV
                     FALEGRRFDLPATLKITEAFRGTVLSRCPEPIPEWVSGHGADGKPTRAPHLALLPLPF
                     VGYEHADGRLLGMAMVIPRGVNGGEAERMLSSVIGFDDYGLPKELRLFGGEWLECVLA
                     LEQREAPPMTLQPDGWVGPAKRWASVTPVVFDRHFDGKNRWEQAAEMIKTGCERVGLP
                     RPEDVILNPVSAHIGVPRSGDFPPMLRKRDGGRLHHMHAIITFVEPILGPVIIGAGRF
                     RGYGLCRPLPQGGVADA"
     gene            complement(2778..4013)
                     /gene="cas7u"
                     /locus_tag="VF226_10230"
     CDS             complement(2778..4013)
                     /gene="cas7u"
                     /locus_tag="VF226_10230"
                     /inference="COORDINATES: protein motif:HMM:NF021156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated RAMP protein
                     Csb1/Cas7u"
                     /protein_id="HEX7074406.1"
                     /translation="MANTNTEALDLASLRKAVAGNAAAFRCLTEYEPAGGPGDKVFPP
                     TYEGGKYAFETRRINGEAVPCVLLDSVQSQANRMELALLQAWEKGDISLPVIVVDFRD
                     GKLFKPLRVTSLDAPHRIADAILRDSEVEEKGKKVRFRDSAKGKALDYVDIRNARPLF
                     ELCPTALVFGMWDSTGPRGGLGAKFQRAVVSEIVGLHAEAGVRTSSRIDPLQIMLQAG
                     PIYAAPEGDWTLDENAARKVKGKPAKVGKEGKPSELNHGNVTPSIAAGGFTIGKAIQT
                     TVISLPALRRLRFPANGETKPEEKTDTAARTVLAALGLCAAALARLDTDLRSRCLLTP
                     TGPVEWELLGEPGETPKRYALSPSTAIEIFRQAVEEAKKAGLPWMEEPLELKPSDSLL
                     ALVRRSQELQVSQAIAEEE"
     gene            complement(4148..>4958)
                     /locus_tag="VF226_10235"
     CDS             complement(4148..>4958)
                     /locus_tag="VF226_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX7074407.1"
                     /translation="VRGRWGEIQLKRVVEMAGMVDHCDFVEQENRTTEEGRLRPDLIV
                     RLPGGKSIVVDAKAPVTAYLEAAEAEEEAVQRERLGDHARQVRDHMIKLGRKAYWEQF
                     DPTPEFVVLFLPGEMFFSAALQQDPALIEFGVGERVIPATPTTLIALLRAVAYGWRQE
                     ALAKNAQEVADLGRQLYERIAKLGEHWANVGDKLARAVDAYNSASSTLESRVLVSARR
                     LRDLKATPEDAEIETLEPLDRTPRVLQAAELAAPAAPAISEDAESAKEGRG"
BASE COUNT          912 a         1642 c         1423 g          981 t
ORIGIN      
        1 tgcgccggta tgtcgcggcc cgtccggttg aggcgaccga accgctggcg cagcgcgtcg
       61 agactcgcac actcgctcac cataccgtcg aaatcgagat tggcgccaac ttccagggtt
      121 tgcgtcgcta cgacgaacac cggccgatcg agtcgtctgt tacctgcgct acgggcgtcc
      181 agcctctcca gccactcctt tacgacatca tcccggtcta gcggccgcat gcggccagtc
      241 aaaagtacgg tatcgtgttg cgctcggttt ttcaggcgtt cgtacacggc ctttgctgtg
      301 gcgacccgat tcactatgat gcctatcgca aggctatcct cggagacgag tcctcgcgca
      361 cgctccacca gttcgtcaac aagggcgttc gcattcgccg acctcgcggc aacgcacaac
      421 gcagctggct tttctgcctt gagcctcgca ccgagaacgg ggtgagcacg gtcgcgggcg
      481 tcatccctga atacatcagt tcgatcttcc ggcggcgtag ctgatagcac taccgtgtga
      541 aacggtgatc gaataggttc atcggcccac tggcgatagc tggcaaccgc ctgcaacgtc
      601 tccaaaaaag ggagcgcgca atgggcttcg tcgaggagaa tgagactatc gttgcaggcg
      661 aggccagcgt ggatcgacca agtacgcgac gagcgtccgt agccgcgaaa gagaaggcgg
      721 gatccgatct gatctacggt actcgagatc acggtcggct gaatgggcgt gcgtgcccag
      781 gcatcatcac ggtaaacccc gcctcggagg tggaagcacg ccagaggctc gtcgccgcca
      841 gcgagtgtcc gcattcgatc cgcgaccttt ttaactattc cacgctttgc tgatcgcagt
      901 ttcatcgcca gcatcatcgc gcgccgatgg gcctcatcca cgatgatacg tcggtccacc
      961 acaaaaaaga ttcgccgcgg ggccgtgcgc tcgctgctgg cgcggttcgc ctggcacgca
     1021 agcgcgaaaa cggcgatgtc gatacaggcc gtcttgcctg cggcagtagg gagagcgatg
     1081 gcagccggcc aggccgcggc tttgcgctcg attacgcgcc tggcgagcct tgattgccag
     1141 gggaagggct ccgcttcata gagcgcccgg aagaactcat cataatggtc tgccgtgagg
     1201 tcaggcatcg gcaacccctc cctgtggcag cggccgacac aggccgtacc cacggaaccg
     1261 tcccgcgcct atgatcaccg gccctaaaat aggttcgacg aaagttatga tggcgtgcat
     1321 gtggtgtagt ctcccgccgt cccgcttccg tagcattggc ggaaagtctc cgctccgtgg
     1381 gacgccaata tgtgcggaca ccggattgag aatcacgtcc tcggggcgcg gaagcccgac
     1441 acgctcgcac ccggtcttta tcatctctgc cgcctgctcc cagcggttct tgccatcgaa
     1501 gtgacgatcg aaaaccacgg gcgttacgct cgcccaacgc tttgccggtc caacccatcc
     1561 atcgggctga agcgtcattg gcggcgcttc ccgctgctcc agcgcgagaa cgcactcgag
     1621 ccattcgccg ccaaacagcc gcagctcctt cggcaggccg taatcatcga aaccaatcac
     1681 gctgctaagc atccgctctg cttcgccccc gttcacgcct cgcggaatca ccatagccat
     1741 gccgagcagc cggccgtcgg catgttcgta acccacaaac ggcaacggta ataacgcaag
     1801 atgcggcgca cgcgtcggtt taccgtcggc accatgtcca cttacccatt ccggaatcgg
     1861 ctccggacac ctgctcagca ccgttcctcg gaatgcttca gtaatcttga gcgtcgccgg
     1921 cagatcgaag cgtcggccct ccagcgcaaa aacaacaagc cgtggatcga acacggtatg
     1981 cggcaagacc ggacgccgct ccggctcggg tcggccgtag ccttgccata ggccgggctc
     2041 gggtcggagg gtgattggag cgccggccgg aaagcgtcgt ttcaattcag cctgcaacat
     2101 tttcttttcc tttccactcg cacggtcaac tcgccgttcg atatccgcgt acgctttcac
     2161 ggcttcccta ttgaaccgat gctcgagata cgtgagccgc cctttaccgc tgatacggag
     2221 ctgaaacctg ggcaccttcg tagtcgccac aaggttcggt gtacgcggca agctttccac
     2281 ccacatctgg acaaatgacg ccgagtggcc aatcgatgtc actttgcggg acaaggacgc
     2341 catcgaatct cggagttccg aaggcacatc gtcgtcccaa accaagtgca ccacgggatc
     2401 gtgcggaata gcgaccggaa acgaccgcgc ctgtcgcgtg cggaattccg gaattaccgc
     2461 gagaccagcg tcttttagtg cacccacggt tgcccggcca ggtctcgatc tgctgacctt
     2521 ctcgtcgtta accgggacgt agctcgtgac gacggtccga tattcagcgt ctgatactgc
     2581 gatggcagga ggaccaagcg tttctagcca ctcgagcgct gcccgttcct ccacgctacc
     2641 atcggtttcg aagtaggccg cggccagcgc catgaatatg cgatcggggt gcggcggcca
     2701 ctcagcctgt tccttcttcg caccgtcggc cgccgccatc gcccacccat tgaggtagcg
     2761 gatgccaagc gcgaacatta ctcctcctca gcaatggctt ggctgacctg caactcctgg
     2821 ctgcggcgca ccagcgcgag caagctatcg gaaggcttga gctccagtgg ctcttccatc
     2881 catggcagcc cggccttttt tgcttcctcg acggcctggc gaaagatttc tattgcggta
     2941 ctcggcgaaa gcgcataacg cttaggtgtc tcgcccggct cacccagtag ctcccactct
     3001 accggccccg tcggtgtcag caggcagcgg gaacgcaggt cggtatccag tcgcgccaaa
     3061 gccgccgcgc aaagcccgag tgctgcaagt actgtgcggg ctgcagtatc ggtcttctct
     3121 tccggctttg tttcgccgtt tgctggaaag cgcaatcggc gcaaagcagg tagcgagatc
     3181 accgtcgttt ggattgcctt tccaatcgta aatcctcctg ccgcaataga cggcgtcacg
     3241 ttgccgtgat tcaactcgga gggtttccct tctttgccca ctttagcggg cttgcctttg
     3301 acttttctgg cagcattttc gtcaagcgtc caatcgcctt cgggtgccgc atatatcggt
     3361 cccgcctgga gcatgatctg caatgggtcg atccgacttg acgtcctgac acccgcctcg
     3421 gcatgaagcc cgacgatttc cgaaaccacc gcccgctgga actttgcgcc gagcccgccg
     3481 cgcgggcccg tcgagtccca catgccgaac acgagcgccg ttggacatag ctcgaaaagc
     3541 gggcgcgcgt tacggatatc tacgtaatcg agcgctttgc ctttggctga atcccgaaac
     3601 cgaaccttct tgcctttctc ttcgacctca ctgtctcgca ggatcgcatc ggcgattcga
     3661 tggggcgcat cgaggctcgt tacgcggagc ggcttgaaaa gcttgccgtc tctaaaatcg
     3721 acgacaatca cgggaagact gatgtcgcct ttctcccatg cttgaagcag cgccagttcc
     3781 atgcggttcg cttgcgactg gaccgagtcg aggagcacgc acggtaccgc ttccccgttg
     3841 atgcgtcgcg tttcgaacgc atatttccct ccctcgtaag tgggcggaaa taccttgtct
     3901 cccggcccac cggccggttc gtattccgtg aggcatcgga aagcagctgc attgccggct
     3961 accgccttcc gtaatgacgc gagatccagt gcttcggtgt tggtgttggc cattggattc
     4021 ctccaaggtc gttgcaaggg tcaaagagac cggagcagaa gccccgggaa atttgcagat
     4081 gctgcggacg caagcatcat tacgggcgaa aaccgcaaac gacggcaccg ccttcggcgc
     4141 cgtaccctca tcccctgccc tccttggcgg actcagcatc ctccgatatc gctggtgccg
     4201 cgggcgccgc cagctccgcc gcctgcaaca cccgcggcgt gcggtcgagc ggctcgagcg
     4261 tctcgatctc cgcgtcttcc ggcgtcgcct tgaggtcgcg cagccgccgc gccgacacca
     4321 gcacgcgcga ctcgagcgtg ctgctcgcgc tgttgtaggc gtcgaccgcc cgggcgagct
     4381 tgtcgcccac gttggcccag tgctcgccga gcttggcgat gcgctcgtag agctgcctgc
     4441 cgagatcggc aacctcctgg gcattcttcg cgagcgcctc ctggcgccag ccgtaggcca
     4501 cggcacggag cagcgcaatc aacgtagtcg gcgtggccgg tatcacgcgc tcgccgacgc
     4561 cgaattcgat cagcgccggg tcctgctgca gtgcggcact gaagaacatc tcgcccggca
     4621 gaaacagcac cacgaactcg ggggtcggat cgaactgctc ccagtaggcc ttgcgcccga
     4681 gcttgatcat gtgatcgcgc acctgccggg catggtcgcc caggcgctcg cgctgtacgg
     4741 cctcctcctc cgcttcggcc gcttcgagat acgccgttac gggcgctttc gcatcgacca
     4801 cgatgctctt gccgcccggc aggcggacga tcagatcggg ccgcaggcgg ccttcttccg
     4861 tggtccggtt ctcctgttcc acaaaatcgc agtgatcgac catgccggcc atctcgacca
     4921 cgcgcttgag ctgaatctcg ccccagcgcc cgcgcacc
//
LOCUS       DASVDU010000162         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1356058, whole genome shotgun sequence.
ACCESSION   DASVDU010000162 DASVDU010000000
VERSION     DASVDU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356058"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VF226_10240"
     CDS             <1..630
                     /locus_tag="VF226_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEX7074408.1"
                     /translation="LLFTIPAAVALVIAAEPIISVLFQRGAFSAADTDATAAALAAFA
                     IGLPAFVMIKVFQPAFFAREDTRTPMWFASINLAVNAVGSAVLFFLFQHLGWMPHVGI
                     AIATTMAGWINAGLLMLALMRSGDYRADRRLIRNTWLILLASVVMGLAIAGILPVMNP
                     YFAREAGLIVRGASLAGLVAAGISIYAMMIVATGVLRIDQLRRFMRRAA"
     gene            713..1750
                     /gene="trpS"
                     /locus_tag="VF226_10245"
     CDS             713..1750
                     /gene="trpS"
                     /locus_tag="VF226_10245"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7074409.1"
                     /translation="MKITPRVFSGMQPTGSLHLGNYLGAMLQWIRLQDTHECIYCVVD
                     LHAITVWQEPTELKNAIRMVTAAYIACGLDPKKSIIFNQSQVHQHAELAWVFNCVARL
                     GWLNRMTQFKEKAGKDRENASVGLFAYPNLMAADILVYRATHVPVGEDQKQHLELARD
                     IAQKFNNDYRDSIVAAGFEDGAFFPLPEPIITGPATRVMSLRDGTKKMSKSDPSDLSR
                     INLMDDADAIARKIQKAKTDPEPLPSEELGLEGRPEAENLVGIYAALAGTDVSSVLRE
                     FGGGPFSTFKKALAEVAVKTIAPIGAEMRRLASDAAEIDRILADGSRRAEAIAAPIMD
                     KVKDIVGFVRR"
     gene            1849..2370
                     /locus_tag="VF226_10250"
     CDS             1849..2370
                     /locus_tag="VF226_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7074410.1"
                     /translation="MTALRMVTKKRRVFEEGHRRKYLVIVDESEEVEAALYFSALRAA
                     HTGGSILLLYVIEPQEYQHWVGVRQVQLEEETNKAKALFRLFRRKLNAAGFESVHTEE
                     VIREGKKSEAILALIDEDEDIAVLTLGAATDAGGPGPLVSSLAAGTAAGSFPIPITIV
                     PGNLTLENLQALA"
     gene            2487..>2934
                     /locus_tag="VF226_10255"
     CDS             2487..>2934
                     /locus_tag="VF226_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015338626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7074411.1"
                     /translation="MFIQTEPTPNPATLKFIPGRPVLGDGTADYRSPEEAAESPLAQR
                     LFEVEGVKGVFLGADFISVTKGDSEWQHLKPAILGAIMEHFMSGAPVMGRESANDAAG
                     ENYDPKDEETVLTIKELLETRVRPAVAQDGGDITFHGYRDGVVFLHM"
BASE COUNT          546 a          929 c          921 g          538 t
ORIGIN      
        1 ctgctgttca cgattccggc ggccgtcgca ctcgtgatcg ctgcggagcc gatcataagc
       61 gttctgttcc agcgcggggc gttctctgcc gctgataccg acgccacggc agcagctctc
      121 gccgcttttg cgatcgggct gcctgcgttc gtgatgatca aggtctttca gcccgccttc
      181 ttcgcgcgcg aggatacgcg cacgccgatg tggtttgcgt ccatcaatct cgccgtgaat
      241 gccgtgggct ccgcggtgct gttctttctg ttccaacacc tcggctggat gccgcacgtc
      301 ggcatcgcta tcgcaacgac catggccggc tggatcaatg ccgggctgct catgctggcg
      361 ctgatgcggt ccggtgacta ccgggccgac cgacggctga ttcgcaacac ctggctgatc
      421 ctgctggcga gcgtcgtcat gggcctcgcg atcgcgggta tcttgcccgt gatgaacccg
      481 tacttcgcac gcgaggccgg tctaatcgtg cgcggcgcga gcctggccgg gctcgtcgcg
      541 gcgggcatca gcatctacgc gatgatgatc gtcgccaccg gtgtgctccg gatcgaccag
      601 ctccgccggt tcatgcgccg ggcagcttga tcaccggcca tgcgatccgt ccacttgcac
      661 cggactgccc ggcgcgccat aacgcccgcg atctcaaaac cacaggaagg ccatgaagat
      721 tacgccgcgc gtcttctcgg ggatgcagcc gaccggctcg ctccatctcg gcaattatct
      781 cggcgccatg ctgcagtgga tcaggctgca ggatacgcac gagtgcatct actgcgtggt
      841 cgacttgcac gccatcaccg tctggcagga gccaaccgag ctcaagaacg ctatccgcat
      901 ggtgacggcg gcctacatcg cctgcggcct cgatccgaag aagagcatca ttttcaatca
      961 gtcgcaggtg caccagcacg ccgagctggc ttgggtgttc aactgcgtcg cgcggctcgg
     1021 ctggctgaac cgcatgaccc agttcaagga aaaggccggc aaggaccgtg agaacgcctc
     1081 ggtcggcctc tttgcctatc cgaacctgat ggcagcggac attctggtgt atcgcgcaac
     1141 acacgtaccc gtcggcgagg accagaagca gcatttggag ctggcacgcg acatcgcgca
     1201 gaagttcaac aacgactacc gcgattcgat cgtggcggcc ggcttcgagg atggagcgtt
     1261 cttcccgctg ccagagccca tcatcaccgg gcctgcgacg cgcgtgatga gcctccgcga
     1321 cggcaccaag aagatgtcga agtccgaccc ttcggacctc tcgcgcatca acctcatgga
     1381 tgatgcggac gccattgcgc gcaagatcca gaaggcgaaa accgaccccg agcccctccc
     1441 ctccgaggag ctggggctcg aaggacggcc ggaagccgaa aacctcgtcg gcatctacgc
     1501 tgccctggcc ggcaccgacg tatcatctgt tctgcgcgaa ttcggcggtg gcccgttctc
     1561 caccttcaag aaggccctcg ccgaggtagc cgtcaagacg atcgccccga tcggtgccga
     1621 gatgcgccgg ctggccagcg atgccgcgga gatcgaccgc atcctcgccg acggttcgcg
     1681 ccgtgcagag gcaattgctg cgccgatcat ggacaaggtc aaggacatcg tcggtttcgt
     1741 ccggcggtaa ggaccacttg ggcgatatgg cagttatctc gataagcgag ctggatcatt
     1801 ccaccgggtg agcttggctg gcgccgggat tgctggcagg cccgcaacat gacagcattg
     1861 cgcatggtca cgaaaaagcg ccgggttttc gaggaagggc atcgacgcaa gtatctcgtc
     1921 atcgtcgacg agagcgagga ggtcgaggcc gctttgtatt tttccgcgtt gcgcgccgcg
     1981 cacacgggtg gatcgatcct gctgctctat gtcatcgagc ctcaggagta ccagcattgg
     2041 gtcggtgtgc ggcaggtgca gcttgaggag gagacgaaca aggccaaagc cctgttccgg
     2101 ctgttccgcc gcaagctcaa cgcggcaggt ttcgaatccg tgcacacgga agaggtgatc
     2161 cgggagggca aaaagtcgga agccatcctc gcgctcatcg acgaggatga ggacatcgcc
     2221 gtgctgacgc ttggcgcggc aacggacgcc gggggccccg gacctctcgt ctcgtccttg
     2281 gcggcaggca cggccgcggg ctcgttcccg attccgatca cgatcgtgcc gggcaacctg
     2341 acgctggaaa acctccaggc gctcgcgtga ggaaagcccg attgcagccc tcgacttttg
     2401 acggggttgg cactagctag tagaataatt ctaaagctgg acgatgccgc ttggcactgc
     2461 cgtgccaggt tcccaaggag atttgcatgt tcatccagac cgaaccgact ccgaatcccg
     2521 ctactctgaa gttcatcccg ggcaggccgg ttctgggtga cggaacggcc gattaccgct
     2581 cacccgagga agcagccgaa tcccccctcg cgcaacggct gttcgaggtc gagggcgtga
     2641 aaggagtttt cctgggggcc gacttcatct ccgtgaccaa gggcgatagc gagtggcagc
     2701 acctcaagcc ggccattctc ggagctatca tggagcactt catgtccgga gcgcccgtga
     2761 tgggtcgcga gagcgccaac gacgcggccg gcgagaacta cgatccgaag gacgaggaaa
     2821 cggtgctgac catcaaggag ctcctggaaa cgcgcgtgcg tcccgcggtg gcccaggatg
     2881 gcggcgacat caccttccac ggctatcgag acggcgtggt gttcctgcac atgc
//
LOCUS       DASVDU010000163         3270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_329078, whole genome shotgun sequence.
ACCESSION   DASVDU010000163 DASVDU010000000
VERSION     DASVDU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329078"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1481)
                     /locus_tag="VF226_10260"
     CDS             complement(228..1481)
                     /locus_tag="VF226_10260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX7074412.1"
                     /translation="MFWPLDIILRRVVVSGNLIVVDAKGDQFQFGDGSGPPVKIRFTS
                     KATERAVAYDPQLALGEAYMDGRLIIEQGNIYDLLDLLMRNAAAQKPARWVASLDVLR
                     RGGRRLQQFNPVSRAQRNAAHHYNIDGAIYALFLDPDRQYSCAYFQPGTDLAEAQLAK
                     KIHLAAKMARSPGQRVLDIGCGWGGLALWLAANTGCRVKGVTLSTEQLAVAQDRARKQ
                     GLSSAVEFALEDYRQVQGKFDRIVSVGMFEHVGVNHYSTFFRQLRHLLDDDGVAVLHS
                     IGRSDGPGYTNPFIAKYIFPGGYIPALSEVLPAIERAGLIVSDVEILRLHYAETLKAW
                     RERFMSRRSEAVALKGEEFCRMWEFYLAGAEGAFRHQGMMVFQIQLVKRIDALPITRD
                     YIFETERRLQHSANAQRTPTRLAGE"
     gene            complement(1654..2229)
                     /gene="rplI"
                     /locus_tag="VF226_10265"
     CDS             complement(1654..2229)
                     /gene="rplI"
                     /locus_tag="VF226_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX7074413.1"
                     /translation="MQVILLERVGRLGQMGDVVNVKDGYARNYLLPKNKALRATKENM
                     EEFQKRRVQLEARNLELKKEAEAVAEKLDGKQFTTIRQAGDTGQLYGSVSTRDIAQLV
                     KEGGFTVDRTQVVLERPIKTLGLHEVRIRLHPEVEVKVTLNIARSQEEADRQARGEDL
                     TVVREERMELETFNPEAAFEEAGEAAGGPEA"
     gene            complement(2265..>3270)
                     /locus_tag="VF226_10270"
     CDS             complement(2265..>3270)
                     /locus_tag="VF226_10270"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX7074414.1"
                     /translation="APSNRPKRGTACVMPFALLIGVGAGLVSGLLFASASTGTLLGLM
                     VLVFLTPLPVAITGLGWGWPSAATAAVTAPVVIAVLLTPRAALLHLIALGLPTVILSY
                     LTLLNRAVSGDGQERVEWYPIGRIVGITAVLAGVIATIALLTTATDVEGLRASLRETF
                     ERGILQQIETAQPGGQTLGENEIATFTELMVVSFAGAIATMWMGVAILNLWLAGVVTR
                     ASGLLVRPWPALSMLTLPRQMAIGFAAAVALTFLPGYPGLIASGFASAFIFAYLLAGL
                     AILHYVTQRMAARPLILFAVYASLVFLNPFSGLAVAMIGLAEPISPLKRKLPPGPPLS
                     "
BASE COUNT          608 a         1046 c         1078 g          538 t
ORIGIN      
        1 cagccgcctg ggcgcgcagc ttgtctgaag ggaggaaggc tgtctgtgtc atgtgcgcga
       61 agctagcaga tcccgtcgcc gatcaggccg ggcaactgca ccggcagccg cgccatcccc
      121 gcaatgcaca cacgccgcca agcgcgcttg cacgatgcga atcggccgat gcgcacagtg
      181 tacgacgttt cggccaggca aaaagcggcg ctcggcgacg ccgtgtatta ctcgcccgcc
      241 aatcgggtcg gcgtccgctg ggcattggcg gagtgctgca gtcttcgctc cgtttcgaag
      301 atgtagtcgc gcgtgatcgg cagggcgtcg atccgcttca cgagctggat ctggaacacc
      361 atcatgccct gatgccggaa ggcgccctcg gcgcccgcga ggtagaactc ccacatccgg
      421 cagaattcct cgcctttcaa tgcgaccgcc tcgctgcggc gcgacatgaa ccgctcgcgc
      481 catgctttca gcgtttcggc gtaatgcagg cgaaggatct cgacgtcgga gacgatcaga
      541 cctgcccgct cgatcgccgg cagcacctcc gacaacgccg gaatgtagcc gcccgggaaa
      601 atgtattttg cgataaacgg gttggtgtag ccgggtccat cggaacggcc gatcgaatgg
      661 aggacggcaa cgccgtcgtc gtcgagcaga tggcgtagct ggcggaagaa ggtcgaatag
      721 tgattgacgc cgacgtgctc gaacatgccg accgagacga tacggtcgaa cttgccctgc
      781 acctgccggt agtcctcgag cgcgaactcc acggcggacg aaaggccctg cttgcgggcc
      841 cggtcctggg cgacggccag ctgctcggtg ctgagcgtca cgcccttgac gcgacagccg
      901 gtgttggccg caagccacag cgccaagccg ccccagccac acccgatgtc gagaacgcgc
      961 tgcccaggcg aacgggccat cttcgcggcg aggtgaatct tcttggcgag ctgcgcctcg
     1021 gcgagatcgg tgcccggctg gaaataggcg caggaatact gccggtcagg gtccaggaac
     1081 agcgcgtaga tcgcgccatc gatattgtag tggtgggccg cgttgcgctg ggcacggctg
     1141 acagggttga actgctgcag acgccggccg ccccggcgca ggacatcgag cgaggccacc
     1201 cagcgtgccg gcttctgggc ggcggcattg cgcatcagca gatcgagcag atcatagatg
     1261 ttgccctgct ctatgatcag ccggccatcc atgtaagcct cacccagggc gagctgcgga
     1321 tcataggcaa ccgcacgctc cgtcgccttg ctcgtgaagc ggatcttcac cggcggaccc
     1381 gagccgtcgc cgaattggaa ctggtcgccc ttggcatcga cgacaatcag gttgccgctg
     1441 acgacgaccc ggcgaagaat gatgtccagt ggccagaaca tggcggggcc cccgggattc
     1501 aacgaggctg ctgcatacga ctcctaacgt tcgacgtagg aaagagtgcc tacttccggc
     1561 gaggacagca agccgtgatt agtgtgcttg tgcaacacac tagcgtgacg agcaggggtg
     1621 attgggggca gacgcctgct gccccccgcg gtatcaggcc tccggtccgc cagcggcttc
     1681 gccagcctcc tcgaatgcgg cttcgggatt gaaggtctcg agctccatcc gctcctcgcg
     1741 cacgactgtc agatcctcgc cgcgggcctg ccggtccgcc tcttcctgcg agcgggcaat
     1801 attcagcgtg accttcacct cgacctcggg gtgcagtctg atgcgcacct cgtgcaagcc
     1861 gagcgtcttg atgggccgct cgagcacgac ctgcgtgcgg tcgacggtga agccgccctc
     1921 cttgacgagc tgcgcgatat cgcgcgtcga aaccgagcca tagagctggc ccgtgtcgcc
     1981 ggcctggcga atcgtcgtga actgcttgcc gtcgagcttc tcggcgaccg cctcggcttc
     2041 cttcttgagc tcgaggttgc gcgcctcgag ctgaacgcgc cgcttctgga actcctccat
     2101 gttctccttg gtggcgcgca gcgccttgtt cttgggcagg aggtaattgc gggcatagcc
     2161 gtccttcacg ttgacgacgt cacccatctg gccgagacgg ccgacacgct ccaacaagat
     2221 aacctgcatg atggcttact ccttcggtct tgatccgtgt ggcttcagga tagcggtgga
     2281 ccgggcggca gcttgcgctt gagcggtgaa atcggctctg caaggccgat catcgctacg
     2341 gccaatccac tgaacgggtt gaggaaaacg agggatgcgt agacggcgaa caggatcagc
     2401 ggccgtgccg ccatcctttg ggtcacgtaa tgcaggatgg ccaagccggc cagcaggtac
     2461 gcgaagatga aggcgctggc gaaccctgat gctatgagac ccggatagcc cggcaggaac
     2521 gtgagcgcga cggccgcggc gaaaccgatc gccatctgcc gcggcaatgt cagcatcgac
     2581 agtgccggcc atggccggac caacaggccc gaggcgcgcg tcaccacccc cgcgagccac
     2641 aggttgagaa tggccacccc catccacatg gtcgcgatgg cgcccgcgaa gctgacgacc
     2701 atcagctcgg tgaaggtggc gatttcgttt tcgccaagcg tctggccgcc gggctgtgcg
     2761 gtctcgatct gctgcagaat gccgcgctcg aaggtctccc ggagcgaggc gcgcaagccc
     2821 tcgacatccg tggccgtcgt gagcagggcg atcgttgcga tgacgccggc gagcacggcg
     2881 gtaatgccga cgatgcggcc gatcggatac cactcgacgc gctcctgccc atcgccagaa
     2941 acagcgcggt tcagcagcgt gaggtaggag aggatcaccg tcggcagacc gagggcgatg
     3001 aggtgcagca gggcggcacg gggtgtcagc aagacggcga tgacgacggg cgccgtgacg
     3061 gccgcggtcg cagccgaggg ccatccccac cctagacccg ttatggcgac cggaagcggc
     3121 gtcaggaaca cgaggaccat cagcccgagc agggtccccg tcgaggcaga cgcaaacagc
     3181 aggcccgaga cgagcccggc tccgactccg atcaggaggg caaacggcat gacacaagct
     3241 gtcccgcgct ttgggcggtt agagggggcg
//
LOCUS       DASVDU010000164         6786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1005543, whole genome shotgun sequence.
ACCESSION   DASVDU010000164 DASVDU010000000
VERSION     DASVDU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005543"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF226_10275"
     CDS             <1..360
                     /locus_tag="VF226_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074415.1"
                     /translation="LSKGWLIAIALFGLAACQTTANSADRLTADQVRATFIGHEWKGS
                     SGTFLFAEDGTWRYRDPRTDVHGTYQIADDGVVCATNAPDSTRAGLTTCYTFYRDGSG
                     YRYWHDRSGRFWPAYVR"
     gene            382..741
                     /locus_tag="VF226_10280"
     CDS             382..741
                     /locus_tag="VF226_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074416.1"
                     /translation="MKTKILNVLAAAFVAATVGASAARAEGDPAAYKAAVPALEQAGG
                     GKACVECFLEKYGPSPFPKAFAVSPDGAYGARYKVGASMAKMREDALASCRKKPAYNP
                     AHDCFIFFENDQQVWKP"
     gene            747..1370
                     /locus_tag="VF226_10285"
     CDS             747..1370
                     /locus_tag="VF226_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074417.1"
                     /translation="MVLRPAMLSLLVALLVTPIHRAFAQAASSGYGGHLFSLGAVIEM
                     VAAFAVVAFFFRKKLHRLHERDRIRLAEIGASTEGIQKLRAQQKGVRIVAIVIAVLAV
                     LATILLLIKINNTSNWALRYDGYVQEQLGWWIMGVGLLWLLAVVVGSVWFMSSRTLGR
                     MVLDRVATIRTRLVEIEKELETRVDQPKADLRSERQLLESELRQLGA"
     gene            complement(1384..2295)
                     /locus_tag="VF226_10290"
     CDS             complement(1384..2295)
                     /locus_tag="VF226_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEX7074418.1"
                     /translation="MTAQRIDVYTQVTNNIIAAIEAGAGDWQMPWHRSGEGLNRPVNI
                     DTNNAYRGINVVSLWAAAQARSFGTGTWGTYRQWQNNGAQVRKGEKASLVVFYREFEV
                     EDWNDETGETERGKRLMARASWVFNADQVDGYAAPSLPEPKDPVQVLEKAERFIAGTG
                     AVVRHGGSRAFYRPSDDIIQIPERERFLGSETSSATESYYATLLHELTHWTGHDRRCD
                     RQFGKRFGDDAYAMEELVAELGAAFLCADLGVTPTPRPDHAAYIDNWLKVLQADKKAI
                     FTAASQAAKATDYLGGLQSADITEAAA"
     gene            2823..4841
                     /locus_tag="VF226_10295"
     CDS             2823..4841
                     /locus_tag="VF226_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEX7074419.1"
                     /translation="MVLGRLFGGKGKGDKPKPLTQAQETRAAFLKSLDPMWREAFLTI
                     YRDPDVDKAFKDEIYVNLQKGELDAARKTIRFVLRDMSPEQQDRVKHAGQHEAWASID
                     TLLDAGIIVPNSETIPVDGVVIGTARHEKDGGASDLVFHGEGHLLTVAPTGAGKGQRF
                     VIPTLLDFEGPVVVLDPKGENYEQTAWRRNLYGQVYKWAPGEPDSDCYNPLDAVRGWD
                     DARHLAELLIIPQSREPFWDNAAKDLLTGLIFYVIRSRPPERRNMREVCRCLTASKTD
                     YEAMVKSLQGSEEERLRELGNTLESQSENLHASIATTLRTQLEAWRADEIVATTSSTT
                     LGWTVETILVKDNIGVTLAAQQGRVPGWHSPASGRYERGMAASVYLIVPPDKIGAYRS
                     VLRVMLGQHLAEAIKWRADIEKNNRLDEGLPESFPDWPMMFYLDELPQLGYMRIVEEA
                     VAITRSYRVRLWLFTQDMAQLKEVYPKWESLLANCRCQIFFRPNEMNTATEIANRLGM
                     RKDIWGGEDWVASPQKLMGPAFREDAVIFLDGLMIRSRMVPPVYANPALQAWIAEQKQ
                     EFGEDIIRAARATPPPSAPDDEPDASPSGGAGSAAPTKSEGSQREDADLADNQEYQRR
                     LAALQAEMRVRKAETSTPGPERSAKPPERPVESSEPPKRPPMPPTFDE"
     gene            4919..5086
                     /locus_tag="VF226_10300"
     CDS             4919..5086
                     /locus_tag="VF226_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074420.1"
                     /translation="MMENIITIAVVVVVGIVVLNLLFWNKNKRAFNNAKQQKIAERQA
                     QRDQAEEYRRK"
     gene            complement(5314..6291)
                     /locus_tag="VF226_10305"
     CDS             complement(5314..6291)
                     /locus_tag="VF226_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HEX7074421.1"
                     /translation="MNHKRKPQDDLFQIEPIDSLRVSPKAQVGQPANDNRPLTRQMEN
                     LFSAAMEIELEAAKEAGALGFMARALVQATMPHKAVAANEFERTNGAFTMTMVAPSKI
                     GLPYGSIPRLVIGFLTTEAVRTKDRHVVLGGSLSRFMTELGLAPTGGRWGSIPRLKDQ
                     MTRLVSCSISCTYTAGSGIGIRNVTIADEANLWWEPRQPDQGSLWESTVVLSEKFFTE
                     ITENPVPIDLRALTALKRSPLALDVYLWLTYRMSYLKDRTAIPWGALQLQFGSDYPTT
                     AQGRRNFKKAFLHALAKVRLIYPALRLDETPEALILLPSLSHVRRQTLA"
     gene            complement(6382..>6786)
                     /locus_tag="VF226_10310"
     CDS             complement(6382..>6786)
                     /locus_tag="VF226_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX7074422.1"
                     /translation="TMRVERTFWEKATAVHVYCLQGRARGDRFARHWYDLVRLDEAGH
                     ADAALKDHDQALAVARHKSMFFAEKDAEGALIDYGAAVRGGLVLVPEGAAFEALAKDY
                     ARMVEDGLLLNDPDRFEDLMESCRQIAGKANR"
BASE COUNT         1348 a         2043 c         2174 g         1221 t
ORIGIN      
        1 ttgtccaagg gttggctgat cgctattgcc cttttcggcc tggccgcttg ccagacaacc
       61 gccaactctg ccgatcgcct gacggcggac caggttcgcg ccacgttcat tggccacgaa
      121 tggaaaggga gcagcggcac attcttgttc gccgaagacg gcacatggcg ttaccgcgat
      181 ccgcggaccg acgtgcatgg aacataccag atcgcagacg atggcgtcgt gtgcgctacg
      241 aacgcgccgg acagcacacg cgccggtctg acgacttgct acacgttcta tcgggacggc
      301 agcggttatc gctactggca cgaccgctcc ggccggttct ggcccgccta tgtccggtga
      361 cccggatcag gagacaacct gatgaagact aaaatcctca atgtacttgc ggctgcattt
      421 gttgctgcca cggtcggcgc aagtgccgcc cgcgccgaag gcgatcccgc cgcttacaag
      481 gccgccgtcc ccgccctcga acaggcgggc ggcggcaagg catgcgtcga gtgtttcctt
      541 gagaaatacg gcccgagccc cttccccaag gcgttcgccg tatcgccgga cggcgcctat
      601 ggcgcgcggt acaaggtcgg tgcatccatg gcaaagatgc gtgaagacgc cctcgcgagc
      661 tgccggaaga agcccgccta caatccggct catgactgct tcatcttctt cgagaatgac
      721 cagcaggtgt ggaagccgtg agggcaatgg tcctgcgacc ggcaatgctg tcgctcttgg
      781 tggcgctgtt ggtcacacca attcacaggg ccttcgctca agcggcgtcc tctggttatg
      841 gcggccatct tttctcgctg ggcgcggtta ttgagatggt cgctgcgttt gcggtcgtgg
      901 ccttcttttt ccgcaagaag ctacacagac tgcatgagcg agatagaatc cgcctcgccg
      961 aaatcggtgc ttctacggaa gggatacaga agctgcgtgc tcagcagaaa ggcgttcgca
     1021 tcgtcgcgat cgtgatcgcc gtgctggctg ttctcgccac catcctgctc ctgatcaaaa
     1081 tcaacaacac atcgaactgg gcgctccgat acgacggcta cgttcaagag caactgggtt
     1141 ggtggatcat gggcgtgggg ctgctttggc ttctggcggt cgtggtggga agcgtttggt
     1201 tcatgtcatc gcgcactctc ggacgcatgg tgctcgaccg cgtggccacc atccgcaccc
     1261 gcctcgtcga gatcgaaaag gaactggaaa cgcgcgtcga tcagccaaaa gcggacctcc
     1321 ggtcagaacg gcaactgctt gagtccgaac tcagacagct gggcgcgtga cagaagaggc
     1381 cgcctaagcg gcggcctcgg tgatgtcggc ggattgcagg ccaccgaggt aatcggtggc
     1441 ctttgccgct tgtgaggcgg cggtgaagat ggccttcttg tcggcctgga ggaccttcag
     1501 ccagttgtcg atgtaagcgg cgtgatcggg acgcggtgtg ggcgtcacgc ccaagtcggc
     1561 gcagaggaag gcggcaccca actcggccac gagctcttcc atcgcatagg catcgtcgcc
     1621 gaagcgcttg ccgaactgcc ggtcgcagcg acggtcgtgg ccggtccagt gggtcagctc
     1681 gtgcaggagc gtggcgtagt agctctcggt cgcggagctg gtttcgctgc cgaggaagcg
     1741 ctcgcgctcg ggtatctgga tgatgtcgtc cgacgggcgg tagaaggcgc ggctgcctcc
     1801 gtggcgcacc acggcgccgg taccggcgat gaagcgctcg gccttttcga ggacctgcac
     1861 ggggtccttg ggctcgggca gcgagggtgc cgcgtagccg tccacctggt cggcgttgaa
     1921 gacccagctc gcgcgggcca tgagccgctt accgcgttcg gtttcgccgg tctcgtcgtt
     1981 ccaatcctct acctcgaact cgcggtagaa caccaccagg ctcgccttct cgcccttgcg
     2041 gacttgcgcc ccgttgttct gccactggcg ataggtgccc cacgtgccgg tgccgaagct
     2101 gcgtgcctgt gcggcggccc agaggctgac gacgttgatg ccgcgatagg cattgttggt
     2161 gtcgatgttg acggggcggt tgaggccctc tccgctgcga tgccagggca tctgccagtc
     2221 gcccgctccc gcctcgatgg cggcgatgat gttgttggtg acctgggtgt aaacgtcgat
     2281 gcgttgcgcg gtcattgttc ctctccttgt cttgcggggc cgcgcccatc gcggccgtcc
     2341 gatggcgacc ggaaaggcag gggcaagacg ggcggcgcat ccgaaggacc ggagaggatc
     2401 ggaggacggc gggcaggctg tttcttggag cgcagcgccc gcgaggaagg tcgaagaccg
     2461 gggaagaaac tgacggaccg ccgttgcgcc gcacggcggc cctgccaggt cagccacccc
     2521 atgaggacgg cccgtggaca gcggtcccag acaaggatct gaggaacacc gcgccgacgc
     2581 cggcggtcgt cacccagaaa acaaaacagc cccgcaaagg tggcggcggg gctgtccgat
     2641 gtgagtggac cgcttgccag cggacaatgg cttatgcgcc ccggaacaag ccgacacctg
     2701 acaaagccgg agagattgag tcgttggccg caaggtgctg aacagggtgt gtaattcggc
     2761 cgatcgggag taaaagattc gggcaggcaa acaagggaga tgtgcagggc gggaggtggc
     2821 gaatggtgct tgggaggctg ttcgggggca aaggaaaggg cgacaagccg aagccgctga
     2881 cgcaggcgca ggagacgcgc gcggcgttcc tgaaatccct cgatccgatg tggcgcgagg
     2941 cgttcctgac gatctaccgc gacccggacg tggacaaagc cttcaaggac gagatctacg
     3001 tcaacctgca gaagggcgag ttggacgcgg cgcgcaagac gatccgcttc gtcctgcgcg
     3061 acatgagccc ggagcaacag gaccgggtca aacacgcagg ccagcacgaa gcatgggcaa
     3121 gcatcgacac gttgcttgat gcaggaatca tcgtgccgaa cagcgagacc ataccggtgg
     3181 acggcgtcgt catcggcacg gcccgccatg agaaagacgg cggcgcctct gacctcgttt
     3241 tccatggcga aggacacctg ctgaccgtcg cccccacggg cgcgggcaag gggcaacgct
     3301 tcgtcattcc gacgctgctg gacttcgagg ggccggtggt ggtgctcgac cccaagggcg
     3361 agaactatga acagaccgcg tggcggcgga acctgtacgg ccaggtctac aagtgggcgc
     3421 caggtgaacc cgattccgac tgctacaacc cgctcgacgc ggtacggggc tgggacgatg
     3481 cgcgccatct ggccgaattg ctgatcatcc cgcagagcag ggaacccttt tgggataacg
     3541 ccgccaagga cctgctgacg gggctcatct tctacgtcat ccgcagccgc ccgccggagc
     3601 ggcgcaacat gcgtgaagtc tgccgctgtc tgaccgcgag caagacggac tacgaagcga
     3661 tggtgaaaag cctgcaaggc tccgaagagg aacggctccg ggagttgggc aataccctgg
     3721 agagccagtc ggagaacctg catgcgtcga tcgccacgac gttgcgtacc cagctggaag
     3781 cgtggcgagc cgacgagatc gtggccacaa catcgagcac gacactgggg tggacagtcg
     3841 agaccatcct cgtgaaggac aacatcggcg tcaccctggc tgcccaacag ggaagagtgc
     3901 cgggctggca ctcacccgca tcagggcgtt acgagcgcgg gatggcggcg tcggtctacc
     3961 tgatcgtgcc gcctgacaag atcggcgcct accgttcggt attgcgcgtg atgcttgggc
     4021 agcatttggc ggaagcgatc aagtggcgcg cggatatcga gaagaacaac aggttggacg
     4081 agggcctgcc ggagagcttc ccagactggc cgatgatgtt ctacctcgat gagctgccgc
     4141 agcttggcta tatgcgcatc gtcgaggaag ccgtcgccat tacgcgaagc taccgggtgc
     4201 ggctgtggct cttcacccag gacatggccc agctgaagga ggtctacccc aaatgggaga
     4261 gcctgctagc caactgccgc tgtcagatat tcttccggcc gaacgaaatg aacacggcga
     4321 ccgaaatcgc caaccgactc ggcatgcgga aggatatctg gggaggcgag gattgggtgg
     4381 cgtcgccgca gaagctcatg ggaccggcgt tccgcgagga tgccgttatc ttcctcgacg
     4441 gcctgatgat ccgatcgcgg atggtgcctc cggtatacgc aaatccagcc cttcaagcgt
     4501 ggatcgccga acagaagcag gagttcggcg aggacataat ccgtgcggca cgagcgacac
     4561 ccccgccgtc tgctcccgat gacgaaccgg atgcatcgcc atccggcggc gcgggatcag
     4621 cggcgccgac caagagtgaa ggctctcaaa gagaagacgc cgacctagca gacaatcagg
     4681 aatatcagcg tcgcctcgcc gccctccagg cagagatgcg tgtccgcaaa gccgaaacat
     4741 cgacccccgg ccctgagagg agcgccaagc cgcctgagcg ccccgtagaa tcatccgagc
     4801 cgcccaagcg ccctcctatg ccgccaacgt tcgacgaatg acctgagagg gctcccagcg
     4861 cgccacacac cctttcaaaa ccacaatccg aaggatcagc acatcaggag tttcagggat
     4921 gatggaaaat atcatcacta tcgccgtcgt ggtcgtcgtc ggcatcgtcg ttctcaatct
     4981 gctcttctgg aacaagaaca agagggcgtt caacaacgcc aagcaacaga agatcgccga
     5041 acgtcaggcg cagcgggacc aggccgaaga atatcgacga aaataagcct cgcggcgctg
     5101 cgaatgctta tccacaagag attcggcaac tacaggattt aataatacag gtacatatag
     5161 gtatatgcgg taccgtttat gcgtaatgcc ggtaccgttt atgcgtgccg agaaagtcgc
     5221 tggcgagaaa agccgccact gtcagggcgg taccgtttat gcgtggataa ctcggcccca
     5281 acccgtttta tgcacagacc ggttagacgt cgttcaggcg agggtttgcc gccggacatg
     5341 ggacagacta ggcaacagaa tgagcgcctc cggcgtctcg tcgaggcgca aggccggata
     5401 gatcagccga accttggcaa gtgcgtgaag gaacgccttc ttgaaattac gcctcccctg
     5461 cgccgtcgtc gggtagtccg aaccgaactg cagttgcagc gcaccccatg ggatcgccgt
     5521 acggtctttc agataggaca tccggtaggt cagccacaga taaacatcga gcgcgagtgg
     5581 cgagcgcttg agtgccgtca atgcccgcag gtcgatgggt accgggttct cggtgatttc
     5641 cgtgaagaac ttctcggaga gcactacggt gctttcccat aaagaaccct gatcaggctg
     5701 ccgaggctcc caccacaggt tggcttcgtc ggcaatggtt acgttgcgaa tgccgatgcc
     5761 gcttccggct gtgtaggtgc aggagatcga gcaggagacc aaccgggtca tctggtcctt
     5821 caagcgaggt atcgaccccc agcgaccacc agtcggcgcc aagcccaact cggtcatgaa
     5881 acggctgagg ctgcctccga gtacgacatg ccggtccttc gtccgcacag cctcggtggt
     5941 caggaaaccg atgacgagac gcgggatact tccgtagggc aagccgatct tcgatggcgc
     6001 gaccatggtc atggtgaacg ccccgttggt gcgctcgaac tcgttggccg caacagcctt
     6061 gtggggcatt gtggcctgca cgagcgcccg cgccatgaag ccgagggcgc cggcttcctt
     6121 ggccgcctcc agctcgattt ccatcgcggc gctgaacaga ttctccatct gccgcgtcag
     6181 cggacggttg tcatttgcag gctggccgac ctgcgccttg ggggaaaccc tcaaactgtc
     6241 gatgggctcg atttgaaaga ggtcgtcttg cggtttgcgt ttgtggttca ttcaccgata
     6301 ttcttatttt gttctttttc agcatatcat aggccgcagt tcgttcagcc atgatttgca
     6361 gttgagacga cggcaagccg actagcggtt tgccttcccg gcaatctgtc gacagctctc
     6421 catcagatcc tcgaagcggt ccggatcgtt cagcagcaag ccgtcctcga ccatgcgggc
     6481 atagtccttg gcaagcgcct cgaacgccgc gccttcgggg accaggacaa gtccgccgcg
     6541 aacggcggcc ccatagtcga tgagcgcgcc ttcggcatcc ttctcggcaa agaacatgct
     6601 cttatgccgc gcgaccgcga gggcttggtc gtggtccttc agcgccgcgt cggcatgacc
     6661 ggcctcgtcc aggcggacga gatcgtacca gtgacgggcg aagcggtccc cacgggcgcg
     6721 gccttgcagg caatagacat gcacggccgt cgccttttcc caaaaggtgc gctcgacacg
     6781 catcgt
//
LOCUS       DASVDU010000165        13261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_2856, whole genome shotgun sequence.
ACCESSION   DASVDU010000165 DASVDU010000000
VERSION     DASVDU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2856"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VF226_10315"
     CDS             complement(<1..933)
                     /locus_tag="VF226_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074423.1"
                     /translation="MSKQQTTGPVLEIEGLRIALPEGADRPLAIDGLNLSVNAGEIVC
                     LVGESGSGKTLAASAVMRLLPEPHVRVTAGTVRFEGQDLFQLSEKQMRALRGSRMSMI
                     FQEPMTALNPQKRVGWQIDEVLRLHTNLSRKERHARVLDILEQVRIPDPASAVNAYPH
                     QISGGQRQRVMIAMALVLQPKLIIADEPTTALDVTTQLQVLRLVRELQAKFGTGVLFI
                     THDFGVVAEIADRVAVLRQGELVEQGPASEVLNRPRHPYTQALIAAVPDLRPPKPKDF
                     SGAPEVLRVANLKKTFKPKRSLFGRRRNAVVAVDD"
     gene            complement(930..1808)
                     /locus_tag="VF226_10320"
     CDS             complement(930..1808)
                     /locus_tag="VF226_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074424.1"
                     /translation="MSLIEAKIEAAPPSAWSELWRRYRRNRAAVLGLVLLGLVLAMAA
                     TAGLVEPEDPLRRAGEPLIPPFTSWATPLGTDQLGRDILAGIFHGARTSLLIGIVSTV
                     IAVSIGILVGSVSGYFGGWIDDTLMRITEAFQTIPNFVLLLALVAIMGSSLQNMIIAI
                     GIVSWTAPARLVRAEFLSLRGREFVEAARNLGVGDAGIIFREILPNALPPVIVFASVI
                     MAISILLESALAFLGLGDPNYASWGNMIGMGRSVLRTDFYCVAIPGIAIIVTVLAFSL
                     IGEGLNEAVNPRQKKS"
     gene            complement(1805..2788)
                     /locus_tag="VF226_10325"
     CDS             complement(1805..2788)
                     /locus_tag="VF226_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074425.1"
                     /translation="MRPAPMLNYLLKRIVQVVPVVLIIMIGTFLLLKLAPGDTVDALV
                     GDMGGADPQVIARLRAEYGLDQPVWVQLLVYMKKLVQFDFGWSFVHEQPVATVLMSRL
                     GPTLLLMLSSLFFAVSIGSVLGMIAARKAYSLTDNVISTLGLIFYATPSFFLGLMMIV
                     LFSVKLGWLPVGGIQTIPSFYSGWDYVIDVAEHLILPTASLSLIYLALYMRLMRTSVL
                     EVADLDYVRTARAKGAGGARLMIHHIARNALLPVITLLGVQFSTILGGSVVIETVFAL
                     PGLGQLAYQAVIQRDMNVLMGLVFLSSVLVIVVNFLIDLIYASLDSRIELS"
     gene            complement(2845..4446)
                     /locus_tag="VF226_10330"
     CDS             complement(2845..4446)
                     /locus_tag="VF226_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074426.1"
                     /translation="MPELTRWLAPSLLGGLLAAGALFPALAADEKPKRGGTLFYHMVS
                     EQRVLNPATRASNGVYEITGKIVEPLIDRTYDGYVGVLATEWSSSEDGKTITFKLRQG
                     VKWHDGKPFTCDDVSFTAMEFWKKLLNYSSTLQQYLEAVDCPDPHTAVFRYSRPMPLS
                     LLVAAMPDLGHPLPKHLYEGTDYLKNPHNTAPVGTGPFKFVQYERGQYVIAERNEDYW
                     RGKEYPYLDRIVWRFIPDRSAAAAALEAGEIHESGFIGISMADIERLSKDPRFDVGTK
                     GYENNVAHSTIEFNHRNKALADIRVRRAIYHGLDIDRAIKTIMRGFAKPGRGPIPSAG
                     GINYTDDVKVYEYDPEKAKALLDEAGYKPGPDGIRLRLRHRPAPWGEYTQLWAEFFAQ
                     SMREIGIDVTLLTNDAPTFLNGVYRDHDFDTANGWHQFRADPAVSTMVWLRSGAPVGT
                     PWSNQFGYKNEKLDKMIDDAASELDPEKRAKMYHEIQRLVMEEVPVIFAIEHPFISVT
                     SKKVKNHHNTPRWNSSSAYDTWLAE"
     gene            complement(4774..5682)
                     /locus_tag="VF226_10335"
     CDS             complement(4774..5682)
                     /locus_tag="VF226_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7074427.1"
                     /translation="MTIATSRQEDAVTTHIGDEGGMGYLERLPRRIVTIYIPLFVFVF
                     VLLFPFYWMAITSVKPNYQLTDYANFSPFWVVEPTLEHIKYLLFETSYPGWLWNTIVI
                     SVASTFVSLAASVFAAYAIERLRFVGAKYVGLAIFLAYLVPPSILFIPLALMVFGFGI
                     YDTKLALIFTYPTFLVPFCTWLLMGYFRSIPYELEECALIDGASRWQILTKVLLPLSV
                     PGLISAGIFAFTLSWNEFIYALTFIQSSENKTVPVGVLTELVRSDVYEWGSLMAGALI
                     GSLPVVILYSFFVEHYVSSMTGAVKE"
     gene            complement(5707..6558)
                     /locus_tag="VF226_10340"
     CDS             complement(5707..6558)
                     /locus_tag="VF226_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7074428.1"
                     /translation="MLPAAVFLLVFLTYPLGLGVWLAFTDTRIGRPGVFIGLENYELL
                     WGDPVFWLSVFNTILYTVVASILKFALGLWLALLLNNHLPFKAFFRAIVLLPWVVPTV
                     LSAIAFWWIYDAQFSIISWVLMKWGLIDTQINFLGDPTNARASVIAANVWRGIPFVAI
                     SLLAGLQTIPPSLHEAATLDGATPWQRFRYITLPMLTPIIAVVMTFSVLFTFTDFQLI
                     YVLTRGGPLNATHLMATLSFQRAIPGGQLGEGAAIAVAMIPFLLAAILFSYFGLQRRR
                     WQQGGAD"
     gene            complement(6865..8172)
                     /locus_tag="VF226_10345"
     CDS             complement(6865..8172)
                     /locus_tag="VF226_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074429.1"
                     /translation="MTISRRNFMIGTGGLVAGAAGLPLIGAARAEMPKYVPEKGASLR
                     MLRWAPFVKGEEDAWLANTKKFTEATGVEVRIDKESWEDIRPKAAVAANVGSGPDIIW
                     VWFDDAHQYPDKLLDVTELAESLGQQYGGWYEGLEGYAKRDGRWIALPLAAIGNAVCY
                     RESWVKEAGFSKFPEKTDEFLELCKALKAKGHPAGFTLGHGVGDGNNFCHWVLWSHGG
                     KMVDENGKVVINSPETIAALRYARELYQTFIPGTESWLDVNNNRAFLAGEVSVTANGV
                     SLYYAAKNDPKLADIAKDIRTTNFPVGPVGKKVELHQTTSAVIFKYTKYPQAALAYLQ
                     FMYDKPQFDAWLEGASAYCCQPLKAYESHPIWKADPIHAPYAQASATLRPNGYAGPLG
                     YASAAVMADYIVVDMVAEAATGQRTPEEAAKRAELRANRYYRV"
     gene            complement(8267..9331)
                     /gene="ugpC"
                     /locus_tag="VF226_10350"
     CDS             complement(8267..9331)
                     /gene="ugpC"
                     /locus_tag="VF226_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7074430.1"
                     /translation="MASVEIRDVRKSFGTTAVIHGVSIDISDGEFVILVGPSGCGKST
                     LLRMVAGLESITAGEIRIGSRVVNNIPPKERDIAMVFQNYALYPHMTVADNMAFSLKL
                     RGAPKSEIQERVNKAADILGLQNLLQRYPRQLSGGQRQRVAMGRAIVRDPQVFLFDEP
                     LSNLDAKLRVQMRTEIKELHQRLKTTTVYVTHDQIEAMTMADKIVVMHDGIVEQMGAP
                     LDLYDRPNNLFVAGFIGSPAMNFIKGKIVSDGSPRVVTESGIALPVNDVPQGSDGRPV
                     IYGIRPEHFVVDSENGIPAEVVVVEPTGSETQVFAKLGGQEIVGVFRERVAVGPGDKL
                     PLAPNPALVHLFDQETGQRI"
     gene            complement(9516..10226)
                     /locus_tag="VF226_10355"
     CDS             complement(9516..10226)
                     /locus_tag="VF226_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7074431.1"
                     /translation="MFRRSCRTCSIRDIAICQALPVSALADLNRMAWRKLVRAGSPIF
                     GAGDDSGIVANVVSGVVRLSRSLGDGRTQIVGLQFAPEFIGRPFAGSSNISAEAATDV
                     ELCCFAKPQFETLLRTHRGLQELLMQRMVQDLDQAREWMLLLGRKTALERVATLLVLC
                     AEKMTGGCSNRGELDGLELELPLSRTEMAEYLGLTLETVSRMLRSLAQSGAIAVHRGR
                     RLTITNASALRRCAELAA"
     gene            10440..12086
                     /gene="ctaD"
                     /locus_tag="VF226_10360"
     CDS             10440..12086
                     /gene="ctaD"
                     /locus_tag="VF226_10360"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX7074432.1"
                     /translation="MTTAAAIHDTHRPHGWRRWLLSTNHKDIGTMYLVFAMIGGIVGG
                     LLSIGMRLELQEPGLQYFSNPHMFNVFTTGHGLIMVFFMIMPSLFGGFGNWFVPIMIG
                     APDMAFPRLNNISFWLLPSAFLLLILSLFVEGEGSCVISLDPAKLDLAGCGAGVGWTA
                     YAPLSTSGHPGPAVDFAILALHIAGASSILSSINFITTIFNMRAPGMTLHKMPLFAWS
                     ILVTAFMLLLSLPVLAGAITMLLTDRNFGTTFYAAAGGGDPLLYQHLFWFFGHPEVYI
                     IILPGFGLVSHVISTFSRKPIFGYLGMAYAMVAIAVVGFIVWAHHMYAAGMSVDAQAY
                     FMFATMVIAVPTGVKIFSWIATMWGGSIRFTVPMLFALGFIFLFTIGGVTGVVLANAG
                     LDRAFHDTYYVVAHFHYTMSLGALFTLFAGWYYWFPKMSGYMYSEFMGRLHFWLTFIG
                     VNLTFFPQHFLGLSGMPRRYADYPDAFALWNKVSSMGSYISALGVLVFFAGIAVAFAK
                     KRQAEANPWGEGATTLEWTLSSPPPFHCFETLPRIKGDQH"
     gene            complement(12312..12386)
                     /locus_tag="VF226_10365"
     tRNA            complement(12312..12386)
                     /locus_tag="VF226_10365"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12352..12354),aa:Val,seq:gac)
     gene            complement(12507..12683)
                     /locus_tag="VF226_10370"
     CDS             complement(12507..12683)
                     /locus_tag="VF226_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074433.1"
                     /translation="MASDNDRTRMNPGDDAPPGTPGTGEDVCPVCRGTGRISNSPCEN
                     CGGTGKVIEGIGGA"
     gene            12959..>13261
                     /locus_tag="VF226_10375"
     CDS             12959..>13261
                     /locus_tag="VF226_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074434.1"
                     /translation="MAIRSRLLAAAVSSSICLTGVASYAQWASARLPDTFASLAGRWS
                     GHGTVKPASGPAESFKCVVTYIPQGSVTRLQQNLRCKSANYKLDAATHLELRGDKVV"
BASE COUNT         2533 a         4204 c         4111 g         2413 t
ORIGIN      
        1 atcgtccacg gcaacgacag cgttgcggcg tcgcccgaac aggctgcgct ttggcttgaa
       61 ggtctttttc aggttcgcga cacgcaacac ctcgggggcg ccggagaagt ctttgggctt
      121 tggcggcctg agatccggaa cggctgcgat cagcgcctgg gtgtacggat ggcgcggccg
      181 gttcaacacc tccgatgctg gcccctgctc caccagctcg ccctgccgga gcacggctac
      241 gcggtccgcg atttcagcca cgacgccgaa gtcgtgcgta atgaacagaa cgcccgtgcc
      301 gaatttggct tgcagctcgc gaacgagccg cagcacctgg agctgcgtgg tgacatcgag
      361 cgccgtcgtg ggctcgtcgg cgatgatcag cttcggctgc agcacgaggg ccatggcgat
      421 catcacgcgc tggcgctggc cgccggaaat ctggtgcgga taggcgttga ccgccgaggc
      481 cggatcggga atgcgcacct gctccaggat atccagaacg cgcgcgtgcc gctcttttct
      541 ggaaaggttt gtatgcagcc gcaaaacctc gtcgatttgc cagccgaccc gcttctgcgg
      601 gttgagcgcc gtcatcggct cctggaagat catggacatg cggctgccgc gcagggcccg
      661 catttgcttt tcggaaagct ggaacaggtc ctgcccctcg aagcgcaccg tcccggccgt
      721 cacgcgcaca tgcggctctg gcagcaggcg catcacagcg gaggccgcca gcgtcttgcc
      781 cgatccactc tcgcccacga gacagacgat ttcccccgca ttcacgctga gattcaagcc
      841 gtcgatcgcc agcggccggt ccgcgccttc cggcagcgcg atccgcaatc cctcgatctc
      901 gagtacgggg cctgtcgtct gttgcttgct catgatttct tctggcgtgg attcacagcc
      961 tcgttgaggc cttccccaat caggctgaag gcaagcaccg taacgatgat cgcgatgccg
     1021 gggatggcga cacaatagaa gtcggtacgc agcaccgatc gccccatgcc gatcatgttg
     1081 ccccagctcg cgtagttggg gtcgccaagg ccgaggaatg cgagtgcgga ttccagcaga
     1141 atggagatgg ccatgatgac ggatgcgaag acgatcaccg gcggcaatgc gttcggcagg
     1201 atttcccgga agattatccc cgcatcgccg acgcccagat tgcgcgccgc ctcgacgaac
     1261 tcgcggccgc gcagcgacag gaattcggcg cgtacgagcc gtgcgggcgc cgtccaggac
     1321 acgatgccga tggcaataat catgttctgg agtgaggagc ccatgatggc gacaagcgcc
     1381 agcagcagaa cgaagttcgg tatcgtttga aatgcctcgg tgatccgcat cagcgtatcg
     1441 tcgatccagc cgccgaaata gcccgatacg gacccgacca ggatgccgat gctgacagcg
     1501 atgaccgtcg agacgatgcc gatcagcagc gacgtgcgtg caccgtgaaa aatgccggcc
     1561 aggatgtctc gacccagctg gtccgtcccg agaggagtcg cccagctcgt gaacggagga
     1621 atcagcggct cgccggcccg ccgcaacgga tcctccggct cgaccaaccc ggccgttgcc
     1681 gccatggcaa gcaccagtcc cagcaggacg aggcccagca ccgcagcgcg gtttcgcctg
     1741 taacggcgcc acaattcgct ccatgcggac ggcggggcag cttcgatctt ggcttcgatc
     1801 aggctcatga cagttcgatc ctcgagtcga ggctcgcgta aatgaggtcg atcagaaagt
     1861 tgacgacgat caccaggacg gagctcagaa agacgagtcc catcagcacg ttcatgtcgc
     1921 gctggatcac tgcctggtag gcgagctggc cgaggcccgg cagcgcgaag accgtctcga
     1981 tgaccaccga accgcccagg atcgtcgaga actggacgcc gagcagcgtg atgacgggca
     2041 gcagcgcatt gcgcgcaatg tggtggatca tcagccgagc cccgccggcg cccttcgcac
     2101 gcgctgtgcg cacataatcc agatccgcga cctccagcac gctggtccgc atcaggcgca
     2161 tgtaaagagc gagatagatg agcgatagcg aagccgtcgg caagatgagg tgctcagcga
     2221 cgtcgatcac ataatcccaa ccggaataga acgaggggat cgtctggatg ccgccgaccg
     2281 gcagccaacc gagcttcacc gagaacagca cgatcatcat caagccaagg aagaaactgg
     2341 gcgtggcata gaagatgagg cccagtgtgc tgatcacgtt gtcggtcagc gaataggcct
     2401 tccgtgccgc aatcattccc agcacggagc cgatgctgac ggcgaagaag agggaggaca
     2461 gcatgagcag cagcgtgggc ccgagccggc tcatcagaac ggtcgccacc ggctgctcgt
     2521 gcacgaacga ccagccgaag tcgaactgga ccagcttctt catatagacc aggagctgca
     2581 cccagacggg ctggtcgagc ccatattcgg cgcgcaggcg ggcgatgacc tgcggatcgg
     2641 cgccacccat gtcgccgacg agcgcgtcga ccgtgtcgcc aggagccagc ttgagcagca
     2701 ggaacgttcc gatcatgatg atcagaacga ccggaaccac ctggacaatc cgcttgagca
     2761 agtagttcag catcggtgct ggccgtatcc gcactccgga ttgcaagctc cggcccgtca
     2821 gcgggccgga gcagagccgt catctcactc ggcgagccag gtatcgtagg ctgacgagga
     2881 gttccagcgc ggcgtgttgt ggtggttctt caccttcttg ctcgtgaccg agatgaacgg
     2941 gtgctcgatg gcgaagatga ccggcacttc ctccatcacg agccgctgga tctcatgata
     3001 catcttcgcg cgcttctccg ggtcgagctc ggaagccgcg tcgtcgatca tcttgtccag
     3061 cttctcgttc ttgtagccga actggttcga ccacggggtg cccacgggcg caccgctgcg
     3121 cagccagacc atcgtcgaga ccgccgggtc ggcgcggaac tggtgccagc cgttggccgt
     3181 atcgaagtcg tggtcgcgat agacaccgtt gaggaaggtg ggcgcatcgt tcgtcagcag
     3241 cgtcacgtcg atgccgatct cccgcatgga ctgggcgaag aactcggccc acagctgggt
     3301 atactcgccc cagggcgccg ggcgatggcg cagccgcagg cgaatgccgt ccgggccagg
     3361 tttgtagccc gcctcatcga gcagcgcctt cgccttctcg ggatcgtact cgtagacctt
     3421 cacatcatcg gtatagttga tcccgccggc cgacgggatc gggccgcggc caggcttcgc
     3481 aaaaccgcgc atgatcgtct tgatggcgcg gtcgatgtcg agcccgtgat aaatcgcgcg
     3541 gcgcacgcgg atgtcggcca gcgccttgtt ccgatgattg aactcgatgg tgctgtgggc
     3601 cacgttgttc tcgtatccct tcgtgcccac gtcgaagcgc ggatccttcg acagccgctc
     3661 gatatcggcc atcgagatac cgatgaaccc cgattcgtgg atttcgccgg cctcgagggc
     3721 cgccgcggcc gccgaacggt ccgggatgaa gcgccagacg atgcgatcca gatacgggta
     3781 ttccttgccg cgccagtaat cttcgttgcg ctcggcgatc acgtactggc cgcgctcgta
     3841 ctgcacgaac ttgaacgggc ccgtgccgac cggcgcagtg ttatgcggat tcttgaggta
     3901 atccgtcccc tcgtaaaggt gcttgggcaa cggatggccg aggtccggca tggcggcgac
     3961 gagcagcgac aacggcatcg gccgcgaata gcggaacacc gccgtgtgcg gatcggggca
     4021 gtcgaccgcc tcgagatact gctgcagcgt ggagctgtag ttcagcagct tcttccagaa
     4081 ctccattgcg gtgaaggaga cgtcatcgca ggtgaacggc ttgccgtcgt gccacttcac
     4141 accctggcgc agcttgaagg tgatcgtctt gccgtcctcc gaggaggacc actccgtcgc
     4201 cagcaccccc acgtatccat cataggtccg atcgatcagc ggttcgacga ttttgcccgt
     4261 gatctcgtag acgccgttcg atgcgcgcgt cgccgggttc agaacccgtt gctccgaaac
     4321 catgtgatag aacagcgttc caccgcgctt gggcttctcg tcagccgcca acgcggggaa
     4381 gagcgcaccg gcagccagca ggccacccag cagtgatggc gcaagccatc gggtcagttc
     4441 cggcatgtcc ctctccgtca cgggaaacaa tgtgtagcac cagacttgtg gcgcgcggct
     4501 gcccccgtgt caagaatgat ttgctctttg cggccgcaat tcagaagcga tattgctcag
     4561 accagccgca gttccgcgac gtcgagagca aattatttca catcgcctct tcagaaggcg
     4621 aggacattcg gaatcctgag tgagcacatg gatgcgctta ccccggagga ggggctgagc
     4681 tatgcgcaat gatgattgca ggtatcgatg cgccacggcg ccgcacggac actgcgcgac
     4741 ggccgctggt gtcgaaccgg aagaagtgga ggcctactcc ttcactgcac cggtcatcga
     4801 cgagacatag tgctcgacga agaaggaata gagaatgacg accggcagcg aaccgatgag
     4861 ggccccggcc atcagtgagc cccactcgta gacgtcgctg cgcacgagct ccgtcaacac
     4921 gcccaccggc accgtcttgt tctcggatga ctggatgaag gtgagcgcgt agatgaactc
     4981 gttccatgac agggtgaacg caaagatgcc ggcggaaatc aatcctggca cggaaagcgg
     5041 cagcagcacc ttggtgagga tctgccagcg cgaggcgccg tcgatcagcg cgcattcttc
     5101 gagctcgtag gggatcgagc ggaaatagcc catcaatagc cacgtgcaga acggcacgag
     5161 gaacgtcgga taggtgaaga tgagcgcgag cttggtgtcg tagatgccga acccgaacac
     5221 catcagcgcc agcgggatga acaggatcga cggcggcacg aggtaggcca gaaagatcgc
     5281 aagcccgacg tactttgccc cgacgaagcg cagccgctcg atggcgtagg ccgcaaaaac
     5341 ggacgcggcc agcgagacga acgtcgatgc caccgagatg acgatcgtgt tccagagcca
     5401 gcccggatag gacgtctcga acagcaaata cttgatgtgc tcaagcgtcg gctcgacgac
     5461 ccagaacggg ctgaagttgg catagtcggt cagctggtag ttcggcttca ccgacgtgat
     5521 cgccatccag tagaacggga acagcagaac gaatacgaag acgaacagcg gaatgtatat
     5581 cgtgacgatc cggcgcggca ggcgctcgag atagcccatg ccgccttcgt caccgatgtg
     5641 cgtcgtgacg gcgtcttcct ggcgggaggt ggcgattgtc atgacttcag tcctctcgca
     5701 acgccgtcag tccgcgccgc cttgctgcca gcgccggcgc tgcaggccga aatagctgaa
     5761 caggattgca gcaagcagga aggggatcat cgccaccgcg attgcggcgc cctccccgag
     5821 ctggccgccc ggaatcgccc gctggaaact cagtgtggcc atcaggtgcg tggcgttgag
     5881 cggaccgccg cgggtcagca cgtagatgag ctggaaatcc gtgaaggtga acagcaccga
     5941 gaaggtcatc acgaccgcga tgatcggagt caacatcggc agcgtgatgt agcggaagcg
     6001 ctgccatggc gtggccccat cgagcgtggc ggcttcatga agcgagggcg ggatcgtctg
     6061 caggcccgcg agcagcgaaa tggcgacgaa aggaatgccg cgccagacat tcgccgcgat
     6121 gaccgaggcc cgcgcgttcg tcgggtcgcc gaggaaattg atctgcgtat cgatcagccc
     6181 ccacttcatg agcacccacg aaatgatcga gaattgtgcg tcgtagatcc accagaacgc
     6241 gatggccgaa agcaccgtcg gcaccaccca cggcagcagg acgatcgcgc ggaagaaggc
     6301 tttgaacggc aggtgattgt tgagaagcag cgccagccac aggccgagcg cgaacttcag
     6361 gatcgatgca acgaccgtgt agaggatggt attgaaaacg gagagccaga agacggggtc
     6421 tccccagagc aactcgtagt tttccaggcc gatgaagaca ccgggccggc cgattcgcgt
     6481 atccgtgaag gcaagccata ccccgaggcc gagcgggtag gtcaggaaca cgagcaggaa
     6541 caccgccgcc ggcagcatga ataggagccc gagcacgtta cggccctggc tcagatcgat
     6601 gcgccgccag ctccgatggg attcagcctt tgctacggcg atgctcatgc tcaccccaga
     6661 aatttgaaag tcgttccgat tgccgctgaa aggatagcac gaggcactgc cttcacccct
     6721 cccaggggga gaggcccgcc caaagggccg ggcgaggggt tgcaacggct acagtcgaaa
     6781 cttcctcgcc tcgaaccctc tcccccaccg gggagaggga gattcgtggc gcgctaatgc
     6841 gtaccggtcg gaaccggagc tcacctagac ccggtaatag cggttggcgc gcagctcggc
     6901 acgcttggcc gcttcctccg gtgtccgctg gccggtggcg gcctcggcga ccatgtcgac
     6961 cacgatgtag tccgccatca cggctgccga cgcgtagccc agcggaccgg cgtaaccgtt
     7021 cggccggagc gtggccgacg cctgcgcata cggcgcatgg atcggatccg ccttccagat
     7081 cggatggctc tcgtaggcct tcagcggctg gcagcagtag gcgctggccc cctcgagcca
     7141 ggcatcgaac tgcggcttgt cgtacatgaa ctgaaggtag gcgagtgccg cctgcggata
     7201 cttcgtgtac ttgaagatca ccgcggacgt ggtctggtgc agctccacct tcttgccgac
     7261 cgggccgacc gggaagttcg tcgtgcggat gtccttggcg atgtccgcga gcttcggatc
     7321 gttcttggcg gcgtagtaga gcgatacgcc atttgcggtc acggacacct cacccgcgag
     7381 gaaggcgcgg ttgttgttga cgtcgagcca gctctccgtg cccggaatga acgtctgata
     7441 aagctcgcgc gcatagcgga gcgcggcgat cgtctccggg ctgttgatga cgaccttgcc
     7501 gttctcgtcg accatcttgc cgccgtggct ccacagcacc caatggcaga agttgttgcc
     7561 gtcgccgacg ccgtgaccca gcgtgaagcc ggctggatgc cctttggcct tgagagcctt
     7621 gcacagctca aggaactcat cggtcttctc ggggaacttc gagaagcccg cttccttgac
     7681 ccagctctcg cgatagcaaa cggcgttgcc aatggcggcg agcggcaggg cgatccagcg
     7741 gccgtcgcgc ttggcgtagc cctcgagacc ctcataccag ccgccgtact gctggccgag
     7801 agactcggcg agctccgtca cgtcgagcag cttgtcggga tactggtgag cgtcgtcgaa
     7861 ccacacccag atgatgtccg ggccggagcc cacgttggcg gcgacggcag ccttcggccg
     7921 gatgtcctcc cagctctcct tgtcgatgcg gacttcgacg ccagtcgctt ccgtgaactt
     7981 cttggtgttg gcgagccagg catcttcctc gcccttcacg aagggcgccc agcgcagcat
     8041 gcgcaacgat gcccccttct ccggcacgta cttgggcatt tcggcgcgcg ccgcaccgat
     8101 gagcggcaaa ccagccgctc ctgcgaccag accgccggtc ccgatcatga aattgcgtct
     8161 tgaaatcgtc atcggtgtgt tcctcctcag cagacgattt actgttgcgg tcagcgtgcc
     8221 cccttgggct gtcggcacac tcgcggacat cttgtggtgg acggcatcag atgcgctgcc
     8281 ccgtttcctg atcgaacaga tgtacgagcg caggattggg cgcgagaggc agcttatctc
     8341 ccgggccgac cgcaacgcgc tcgcggaaca cgccgacgat ttcctggccg ccgagcttgg
     8401 cgaagacctg cgtctccgag cccgttggct cgacgacgac gacctcggca gggatgccgt
     8461 tctccgaatc gacaacgaaa tgctctggcc ggatgccata gatcaccggc cgcccgtccg
     8521 agccctgcgg cacgtcgttg accggcagcg cgatgccgct ctcggtcacg acacgcggcg
     8581 agccgtccga gacgatcttg cctttgatga aattcatggc cggcgagccg atgaagccgg
     8641 caacgaacag gttgttggga cggtcgtaaa gatcgagagg ggcgcccatc tgctcgacga
     8701 tgccgtcgtg catcaccacg atcttgtcgg ccatggtcat ggcctcgatc tggtcgtgag
     8761 tgacgtagac cgtggtcgtc ttcaggcgct gatgcagctc cttgatctcg gtgcgcatct
     8821 gcacgcggag cttcgcgtcg aggttcgaca gcggctcgtc gaacaggaag acctgcggat
     8881 cgcggacgat ggcgcgaccc atggcgacgc gctggcgctg accgcccgag agctgacgcg
     8941 gatatctttg gagcaggttc tgcagcccca gaatatcggc cgccttgttc acgcgctctt
     9001 gaatctcgct cttcggcgcg cccctgagct tgagcgagaa tgccatgtta tccgctacgg
     9061 tcatgtgcgg ataaagggcg tagttctgga acaccatcgc gatatcccgc tccttgggcg
     9121 ggatattgtt gacgacgcga ctcccgatgc ggatctcgcc ggctgtgatg ctctcgagac
     9181 cggccaccat ccgaagaagg gtggacttgc cgcaaccgga tggccccacg aggattacga
     9241 actcgccatc agagatgtcg atcgatacgc catggatgac agccgtcgtc ccgaaggact
     9301 ttcggacatc cctgatctca actgatgcca ttgccggcca cttctcccct ggccattagc
     9361 tgccattctg acagctcacc tgccataccc ttcggctggt aagctatcaa gaagtcagct
     9421 agctaccaca atgtcggtgg atttcaacag cttgaacctt aacggccgtg cggccgccac
     9481 ataccgatac gataatctcg gttaaggaat aacaatcaag ctgccaactc agcgcaccgg
     9541 cgcaacgcgc tggcattcgt gatcgtgagc ctgcgtcccc ggtggacggc aatggcgccg
     9601 gactgggcca ggctgcgcag catccggctg accgtctcaa gagtgaggcc gagatactcc
     9661 gccatttcgg tgcgcgacag cggcagctcc agctccagcc catcgagctc accgcgattc
     9721 gagcagccgc cagtcatctt ctcggcgcag agcaccagaa gcgtcgcgac gcgctcgagg
     9781 gcggtcttgc gaccgagcag cagcatccac tcccgcgcct gatcgaggtc ctgaaccatg
     9841 cgctgcatga gcagctcctg caagccgcga tgggtacgca ggagcgtctc gaactgcggt
     9901 ttggcaaagc agcacagttc gacgtcagtg gccgcctcgg ccgaaatgtt gctgcttccc
     9961 gcgaacggtc gaccaatgaa ttcgggcgcg aactgaaggc ctacgatctg tgtccgcccg
    10021 tctcccagcg agcgcgaaag gcgcacgaca cctgaaacga cattcgccac gatgcccgaa
    10081 tcgtcgcccg cgccaaagat cgggctgccg gcgcgcacga gcttccgcca ggccatgcgg
    10141 ttgaggtcgg cgagcgccga gaccggtaag gcctggcaga tggcgatgtc gcgaatcgag
    10201 caggtccggc aacttctgcg gaacatcccg cgcgcactcc gtggcaccgg catggattgg
    10261 ctcggcgctc ggcgaaacgc tcaatcacac ccggcctgga aattctaatg cgacaggccg
    10321 ggtccgacag gatcagtaca tttcgccgca tccggatctg atctggatca agcgcgcgtg
    10381 tcgcccttgc cgcagatttg tcgcttccga cgcatgcatc caaacgggga gacgccgcga
    10441 tgacaacggc tgcagccatc catgacactc atcgtccgca cggctggcga cgctggttgt
    10501 tgtccaccaa ccacaaggac atcgggacga tgtacctggt cttcgccatg atcggcggga
    10561 tcgtgggcgg gcttttatcc atcgggatgc ggctggagct gcaggagccg gggctccagt
    10621 acttctcgaa tcctcacatg ttcaacgtgt tcacgaccgg acacgggctg atcatggtgt
    10681 tcttcatgat catgccgtct ctgttcggag ggttcggcaa ctggttcgtg ccgatcatga
    10741 tcggcgcgcc cgacatggca ttccctcgtt tgaacaacat ctcgttctgg ctgttgccgt
    10801 cggccttcct gctgctgatc ctgtcgctgt tcgtggaggg tgagggctcc tgcgtcatca
    10861 gcctcgaccc ggccaagctg gatctggcgg gctgcggcgc tggcgtcgga tggacggcct
    10921 atgcgccgct gtccacgtcg gggcacccgg gccctgccgt cgacttcgcc attctcgcac
    10981 tgcacatcgc gggtgcgtcg tcgatcctca gctcgatcaa cttcatcacc acgatcttca
    11041 acatgcgggc accgggcatg acactgcaca agatgcccct gttcgcgtgg tcgatcctgg
    11101 taacggcgtt catgctgttg ctgtcgttgc ctgtgctggc cggcgccatc accatgctgc
    11161 tgaccgaccg caacttcggc acgacgttct atgcggcagc cggtggcggt gatccgctgc
    11221 tctatcagca ccttttctgg ttcttcgggc atccggaggt ctacatcatc atcctgccgg
    11281 gcttcggact ggtcagccac gtcatctcga ccttctcgcg gaagccgatc ttcgggtacc
    11341 tcggcatggc ctatgcgatg gtcgcaatcg ctgtcgtcgg cttcatcgtc tgggcccacc
    11401 acatgtacgc cgcgggaatg tccgtcgacg cgcaggcgta cttcatgttc gcgaccatgg
    11461 tgatcgcggt gcccacgggc gtgaagatct tttcctggat cgcgaccatg tggggcgggt
    11521 ccatccgctt cacggtgccg atgcttttcg ccctcgggtt catcttcctg ttcaccatcg
    11581 gcggcgtaac cggcgtcgtg ctggccaatg ccggcctcga cagggccttc cacgacacct
    11641 actacgtggt cgcgcatttc cactacacga tgtcgctcgg agcgctcttc acgctgttcg
    11701 ccggttggta ctactggttc ccgaagatga gcggctacat gtactccgag ttcatggggc
    11761 ggctgcactt ctggctgacg ttcatcggcg tcaacctcac gttcttcccg cagcacttcc
    11821 tagggctgtc gggcatgccg cggcgctatg ccgattaccc cgacgcgttc gccctctgga
    11881 acaaggtctc ctcaatggga tcctacattt ccgccctggg cgtcctggtc ttcttcgcgg
    11941 gaattgccgt agcgttcgcg aagaagcggc aggccgaggc aaatccatgg ggcgagggcg
    12001 caaccacgct cgagtggacg ctgtcctcac cgccgccgtt ccactgcttc gagacgctgc
    12061 cgcggatcaa gggcgaccag cactgatttc gaacgagccc cggcgccaag cttcggactg
    12121 aagcgccagg gctgaaccaa tgacatcaaa tggaaatggc ggcggcacct cggttgccgc
    12181 cgcttttgcg actggaacac gatcaggcag gagatcgccg cccgcgcggc agccccgatc
    12241 gccaggcaaa agcgcgctgc gagccgacgt gatagccgct tgggcgaacc gcttggcttt
    12301 ggaggaaaaa atggtgggcg cgacagggat cgaacctgtg acccctacca tgtcaaggta
    12361 gtgctctacc gctgagctac gcgccccttg aactgcctgc ttcctagccg ttccaggcgc
    12421 atgctgcaag cgggttctgc gccgccaaat ggcacaaggc cagaaattgc gacgccccaa
    12481 aatcaggacc agcgatcctc cgctcgtcag gcgccgccaa tcccctcgat caccttgccc
    12541 gtgccgccgc agttctcgca cgggctgttg ctgatccgtc ccgtgccgcg gcagaccggg
    12601 caaacgtcct cgccggtccc cggcgtgccg ggcggagcgt cgtcgcccgg gttcatgcgg
    12661 gttctgtcgt tgtcgctcgc cacgaagctt tctcctgctg tcccatttct cccgcaatcg
    12721 ccgccgaggc caaatcgttc cgatcggaag accggcagcg cggccgtcga aaattcaaga
    12781 gttgaatgga caacaagttg ccgaaatcgc gaagcagtgt gcgcatcggc agggtgcaga
    12841 tcacaagaac ttgggcctgc cacgcattgt tccaattcag ctctggcgcc cgtccgccga
    12901 tcccgaggca ttccggtggc ggcagcgcgc cacgtaagag gtccaggaga actcgcccat
    12961 ggcgatccgc tcgcgcctac tcgccgctgc cgtctcttcc agcatttgcc tcaccggcgt
    13021 cgccagctat gcacagtggg cttcggccag actgccggac accttcgcgt cccttgcagg
    13081 ccgctggtcc ggccatggca cggtgaagcc ggcatccggt ccggccgaga gcttcaaatg
    13141 cgttgtgacc tacatccccc aaggcagtgt cacgcggctt cagcagaacc tgcgctgcaa
    13201 gagcgccaac tacaagctcg acgcggcgac ccatctcgag ttgcgcgggg ataaggttgt
    13261 c
//
LOCUS       DASVDU010000166        27863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_606357, whole genome shotgun sequence.
ACCESSION   DASVDU010000166 DASVDU010000000
VERSION     DASVDU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 27863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27863
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606357"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VF226_10380"
     CDS             complement(<1..561)
                     /locus_tag="VF226_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX7074435.1"
                     /translation="MIFGLTIAVIFGAVGGAIDYGRWLTARTQTQNAVDAAVLAAGRV
                     LQASGGNTEAALQAARTYYEEMKPDATNDESVHFELADDGSAIVASTVGNVTTPFLSA
                     VGIKSMPVRTSAKAVLAVGGNAETNLEISMMLDITGSMSGSKIQDLKLAAKDLINIVV
                     WANQDEYTSRVAIAPFAPRVNVGDYVS"
     gene            complement(925..2838)
                     /gene="cysN"
                     /locus_tag="VF226_10385"
     CDS             complement(925..2838)
                     /gene="cysN"
                     /locus_tag="VF226_10385"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEX7074436.1"
                     /translation="MQQHVPLDLTGRLRQGDGSAEDKTLLRFITCGSVDDGKSTLIGR
                     LLYDTRLIFEDQVAKLARDSKKHGTTGDDLDLALLVDGLQAEREQGITIDVAYRFFAT
                     EKRKFIVADTPGHEQYTRNMATGASTADLAIILVDARKGVLQQTRRHSRIAALLGIRH
                     VVLAVNKIDLAQYDEGVFNRITEAYRAFADHLNFKSLIAIPISARFGDNVIARSPEMP
                     WYRGPVLLEHLERVEVEADAASKPFRMPVQWVNRPNHDFRGYSGTIASGRVRAGDRII
                     IANSGRETRVRSIVTYDGTMAEAVAGDAVTLTLTDEVDISRGDLIAAPDERPEVSDQF
                     AAHIVWMNDEPLIPGRPYLLKIGARTVTASVTEIKHQEDVNTFQKLAAKTLKLNEIGF
                     VNIETHEPVAFDPYEENRETGAFILIDRLSNLTVGAGMIQFGLRRATNVHWQALDVSK
                     ASRAELKGQRPCCLWFTGLSGSGKSTIANLVEKKLHAHGRHTFILDGDNTRHGLNRDL
                     GFTDADRVENIRRAAHVARLMVDAGLIVLVSFISPFEAERRMARELFDNGEFIEVFVD
                     TPLEICEERDVKGLYRKARAGQIQNFTGISSRYEPPQSPELHLQAGTVPADILADEIY
                     RYLQSLGYLGATR"
     gene            complement(2838..3752)
                     /gene="cysD"
                     /locus_tag="VF226_10390"
                     /pseudo
     CDS             complement(2838..3752)
                     /gene="cysD"
                     /locus_tag="VF226_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
     gene            3884..6373
                     /locus_tag="VF226_10395"
     CDS             3884..6373
                     /locus_tag="VF226_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074437.1"
                     /translation="MIMDKNGSGLPAEAKAIIVGGGVVGCSVAYHLAKLGWQDVILLE
                     RKRLTSGTTWHAAGLIGQLRATLNLTKLAKYSAELYLGLEAETGIAIGFRQNGSLGVA
                     LSNDRYEEFARGASMARTFGLEAHMLTPAECREKYPLLNLNGVVGGIFLPTDGQADPA
                     NIALALAKGARQSGVRILEGVRVTGITKAKGRVTGVVTEHGTIRAEYVVNCAGLWGRE
                     VGRMAGVNVPLQASEHFYVVTEANSAIPKDLPVLRVPDECAYYKEDAGKLLVGFFEPV
                     AKPWGMDGVPEDAEFLTLPDDWEHIAPQLEKAMERVPLLAETGIHTFFNGPESFTPDN
                     RYLLGEAPELRNFYVAAGFNSIGIQSAGGAGMALAHWMQDGEPPFDLGDVDIRRMFPF
                     QGNRTYLVNRVTETLGLLYADHFPYRHYESARGVRHLPLHERHAAFGACFGEAAGWER
                     PFWYLPAEARARGERPEYRYSWKRQNWFAYAAEEHAAVRNGVGLFDLTPFGKIRVEGR
                     DAEAVLQRICANDVAVEPGRIVYTQWLNQKGGIEADLTVTRLSETEFLVVTSSGTLPR
                     DLNWLKRHIPEDAHCIATDVTSAEACLAVMGPRSRELLAPIVDADLSNEAFPFGTARD
                     IEIGMGLARAHRVSYVGELGWELYVPTDMARHVFDVIAERGSAVGLRLCGMHALDSCR
                     IEKAFRHFGHDISPEDHVLEAGLGFAVRTDKPDGRFGPFIGREAVLRKKEAGLTKRLV
                     QFQLVDPEPLLYHNEPILRDGKVVGHLTSGAYGHHLGAAIGLGYVPTSPQESAQELLA
                     SRYAIDIAGKIVPARASLKPLFDPTGARMRG"
     gene            complement(6370..7413)
                     /gene="lpxK"
                     /locus_tag="VF226_10400"
     CDS             complement(6370..7413)
                     /gene="lpxK"
                     /locus_tag="VF226_10400"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEX7074438.1"
                     /translation="MPLDEPGWWYRPGSAPAVVRALRPVSAAYAWAVERRFRKTKPFR
                     SSLPVICIGNLTVGGTGKTPFAIHVAETLARLGERPVLLTRGYGGRIRGPHWVDPERD
                     TAADVGDEPLLLTRIAPTLVSRDRAAGARAIEAAGSATVIVMDDGLQNPSLVKDLTLA
                     LIDAHRGIGNGKVLPAGPLRARLAFQLAMLDAIVVNRPPGAARDANLDWLGSFTGPIL
                     SAAPEPQGDLDWIKGARLVAYAGIANPQRFFALLQELGGDLVATVALADHHAFGAKDA
                     KRILDLAASRDAIPVTTEKDWVRLRGGGGPLAELATRSRTLAIRAKLTEQDGARLQDL
                     LSSALVRSRRRPA"
     gene            complement(7403..9430)
                     /locus_tag="VF226_10405"
     CDS             complement(7403..9430)
                     /locus_tag="VF226_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7074439.1"
                     /translation="MGLFSVDKARLNRLAGRAIAHYVDFVHRTSRIVNEPSDIVSYVR
                     LHSPFILAMWHGQFLMLPRVERGGLNVRSMVARHGDAELIGQVLDYFKMDMIRGAGAG
                     YRRRDRGGAQALRAALRALEDGDCVAMTADVPPGPARIAGEGIVTLARLSGRPIIPAA
                     VATSRYKAFDTWSRMTLNLPFGTLAVVAGEPIHVGRHDDRESLERARIRVQDGLNAVT
                     RRAYELAGADPTRATPLLPGEVAPPVQPGLSLKAYRALTSLARPALPLILDARARRGK
                     EDEARRGERLGKPSAARPDGCLIWIHAASVGETNAVLPLLKALRAERPDIRFLLTTGT
                     VTSAEVAARRLGPEDVHQFVPLDAPDVVARFLDHWRPQLAVFTESEIWPNLILESAAR
                     GIPLALINARMSNRSFDRWRKNKGMARPLFSRFDLVIAQNGKLARRFRELGARNVQFV
                     GNLKIDAPAPEADPVEVRRLRDALAGRPCFIAASTHEGEDEAIGAAHKILKQSFPRLC
                     TMLVPRHPERGGAIAELLRGEGLNVAQRSRGALPDDATDIYVADTLGELGTFYSLSPV
                     AFVGGSLVSRGGQNPIEAIRHGAVVITGPHWFNFGDAYSALIRRGAACQVASAEELAH
                     AVKSLLDNEVELVRMRTAAQAVLSDLSGALRRTVEALLPLLHHKEGLRRAS"
     gene            9593..10336
                     /locus_tag="VF226_10410"
     CDS             9593..10336
                     /locus_tag="VF226_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEX7074440.1"
                     /translation="MANLSLAKRTRRRVNRLVSSCRHTLVESSPGWAKTAFGPAASYF
                     DMLFVDHGIFRLFYLNRHRLGEQAWRSAQPAPHQIRAMARDGIRTIVNLRGEHPCGSY
                     WLEEEACRRYGIALVNFRVRSRAAPSRSELRGARELFERIEYPMLMHCKSGADRAGLM
                     SVLYRHFREGVPLEIAKKELSLRFGHIRQADTGVLDYFFDRYLQDTAERPMPFLEWVD
                     KVYDPKELKRTFMAKGWANKLVNDILRRE"
     gene            complement(10386..10664)
                     /locus_tag="VF226_10415"
     CDS             complement(10386..10664)
                     /locus_tag="VF226_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEX7074441.1"
                     /translation="MSRADPTRKPPKKQLLHLVFGGELVSLDSVEFRNLDDLDIVGIY
                     PNYAEAYKAWKAAAQRTVDNAHMRYFIVHLHRLLDPDPAPIPGSNPSH"
     gene            complement(10769..11221)
                     /locus_tag="VF226_10420"
     CDS             complement(10769..11221)
                     /locus_tag="VF226_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEX7074442.1"
                     /translation="MTIRSLVGTIVAVAGLAFGLGATASGQTIEVFKSATCGCCSGWI
                     AHIEQNGFSAKAQNLAAGQLARVKADAGLSPDLQSCHTAKIGGYVIEGHVPAEDIRRL
                     LAEKPDVIGLTVPGMPLGSPGMEAGNEKEPFEVLLVRRDGKTEVFSRH"
     gene            11546..13453
                     /locus_tag="VF226_10425"
     CDS             11546..13453
                     /locus_tag="VF226_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX7074443.1"
                     /translation="MAHHRVSPSTAPAGEDASPKQLVGLTFGSIGVVYGDIGTGPLYA
                     FREATQATVSAGLPPQEAVLGVLSLILWALFVIVTLKYVFILLRLDYHGEGGTFALMA
                     LGQSAAGKGAYAILVLGVAGASFFFGDAVLTPAISVLSAVEGAKLIAPALDNVVLPVT
                     IAIISGLFWFQSRGTASVARFFGPAMAIWFALLALGGLKHIVDDPHVFLALNPFYAIT
                     FVASHGMTSLAVLGLVFLAVTGAEALYADLGHFGRKPIQHAWLGLVLPALALNYLGQG
                     ALVLARPEAIANPFYLLYPSWALAPMVVAATLATVIASQAVITGAYSMTRQAVQLKLL
                     PRFDIRHTSETVAGQIYLPRINVLLFIGVVLVVMLFRSSSNLASAYGVSVSAEMVITS
                     SIAFFVICYLWRWPRWQAAALIGPLLLIELVFFGSNMLKVLEGGWLPLLIASAVATVM
                     FTWIRGARLIIETLRKTETDLDWLVRKLEAKPPHRVPGTAVFLTAGANLAPISLLHNL
                     KHNRVLHRRNIILTIRSEEVPRVPASERITIEPVGDGFIRVIARYGFMQTPSIPRIFA
                     ACERKGLNVDVATASYFLSRRSLKPKRKSKMPHWQQILFIRLAHTAQDATGYFKIPTD
                     RVVEIGAQVAV"
     gene            complement(13454..14749)
                     /locus_tag="VF226_10430"
     CDS             complement(13454..14749)
                     /locus_tag="VF226_10430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074444.1"
                     /translation="MSQRVKGGPAQWAWVVWLVSPIGTAIVALGITQIVAWGTTLYAL
                     GVLASPIAEDTGWSRSLVFSGLTIGLLASSFVSTAVGKAIDTQGGRLVMSVGSILCAI
                     SLLIVAAASNPIVYLAGWAAMGVGMRMSLYDAAFAALVQVAPHRGRRAISYLTLFGGF
                     ASTFFWPIGHILTAEVGWRMALVIYAGFNLAVCLPLHWWGLARREPPAPAAAISGAPA
                     SAAAGMSDPPLDGQARTLAMVLFGLVMSASAFVFGAMAVHLPAILEANGLSAAAAVTL
                     ASFKGVAQVAGRVAEILFGKRLHAIDLGRISVAGMPLAFIALMAGGASTSAALLFIIL
                     FGVANGLVTIVRGAVPLAIFGPQGYGAVLGILATPYLLVNAIAPAVLAVVVDLWGYTV
                     AEAVLLAAGLLTAGSMEIMSFWYRRRQKRDDRGAGVQAT"
     gene            14918..16276
                     /locus_tag="VF226_10435"
     CDS             14918..16276
                     /locus_tag="VF226_10435"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEX7074445.1"
                     /translation="MNDSEENRFAARAARYVRVGTNVGAVAARVAGQRLLGIRGDRSK
                     NAMDLAVALGGLKGPLMKVAQLLSTIPEALPPEYARELSQLQSQAPPMGPAFVRRRMM
                     AELGPDWASRFKSFETTAAASASLGQVHKAISHDGRELACKLQYPDMQSAVEADLNQL
                     KIIFSIHARMDPAVDTREIMTEIAARLREELDYELEARHMRLYAHIFENDPLIRVPLV
                     ESELSTKRLLTMTWLEGKPLLSYKEAPLEHRNTIARAMFRAWWYPFSHYGVIHGDPHL
                     GNYTIFEQDGAPAGINLLDYGCIRTFRTPFVQGVIDLYTGLLTGNQDLVVHAYETWGF
                     KGLTNELIEAMNIWARFIYGPLLDDRERTIAEGTTPGEYGRREAFQVHKVLREKGPVV
                     VPREFVFMDRAAIGLGGVFLHLDARMNWYRLFNETIEDFDLASVAERQKTAFTAAGVP
                     LP"
     gene            complement(16304..17518)
                     /locus_tag="VF226_10440"
     CDS             complement(16304..17518)
                     /locus_tag="VF226_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7074446.1"
                     /translation="MKITIPDFCLVAVVGASTESTTAFMRRHFAAEEILDRSSETWPL
                     IAADRLACRELVAINASETVTQQQIDLLRLAKKFYAQPVAIVLCTPGDRADLRLVRSL
                     EATGFKPTHPIAPQDAEAVSITRERLPPDLRHEHGPFDIIGDVHGCADELTELMQRLG
                     YNKLRITGVGPDRRAEVETPPGRRLIFVGDFVDRGPRSPDVLRIAMSMVEKGQAFAVP
                     GNHDVKFLRWLRGANVRLTHGLDKTVAQFSEESGRFRAQVREFLLSLPSHLWLEDGRL
                     VVAHGGILEEMIGRESEAVREFCLYGDTDGETDETGLVVRYHWAANYRGETTIVYGHT
                     PVPQADWVNNTLCIDTGCVFGGKLTALRWPEREVVSVPSAQMYAKPGRPFGHPPVRPV
                     HRERPPQRRRPR"
     gene            complement(17597..18976)
                     /gene="glcF"
                     /locus_tag="VF226_10445"
     CDS             complement(17597..18976)
                     /gene="glcF"
                     /locus_tag="VF226_10445"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX7074447.1"
                     /translation="MQTNFTAEQLEDPRIEEIDRIVRRCVHCGLCTAVCPTYVLLGDE
                     RDSPRGRIYMMKDMFERGVPASPEVQQHVDRCLSCLSCMTTCPGGVDYMHLVDHARAH
                     IENTGQRSLKQRLMRKLLAEVVPYPDRFRAVLRLAPLGLPLRGLMRRLGLKELVAMLD
                     LAPSRSLRSPKYSGPGTAVTKAERKGRVIMLAGCAQQVLRPEINDATIRLLARRGVDV
                     EVAPGAGCCGALVHHMGREAEAVEFAKRNVDAWWKVMSRDRIDALIINASGCGTMVKD
                     YGHLLARVEGYAERARKIADMTRDVTEFLSEYDLGPPKRWSSLRVAYHSACSLQHGQR
                     VTEEPKQLLRDAGFTVVDVPEGHICCGSAGTYNILQPDLARQLRDRKVGHIESLRPDL
                     VAAGNIGCITQLATGMDIPIVHTIELLDWAYGGPVPRGLERFESLVSDVPEPQNAMAK
                     RSLQDFLRV"
     gene            complement(19008..20225)
                     /locus_tag="VF226_10450"
     CDS             complement(19008..20225)
                     /locus_tag="VF226_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7074448.1"
                     /translation="MATVRPAAEWELSNLILAAAERGKPVEIMGAGTKRGVGRPVQAA
                     VTISTAGLRGVTLYEPTELVMSARAGTLVSDIESQLAEKGQMLPFEPIDLGPALGGQA
                     GRGTIGAVFATNLSGARRVATGSARDHLLGVHAINGRGEAFKSGGRVLKNVTGYDVAR
                     GVAGSWGTLAVLTEVTFKVVPRPERTATVALFGLTDDLATEVMCQAMGTPYEVSGAVH
                     LPQPMAQRLLTPEFREPGKAVTALRIENLASFVAYRCDKLRENLAPYGKAEVLGDEVS
                     RAFWGELRRLSVLQQDSRPLWRISTKPTNGYRVVEAIRRYMPVDAFYDWSGGLIWLEL
                     PEAADAGASDIRRVIAQFGGHATLMRAEPAVRSAIDVFQPLEPGVERLTRGIKSAFDP
                     AGILNPGRMYAAI"
     gene            complement(20240..21688)
                     /locus_tag="VF226_10455"
     CDS             complement(20240..21688)
                     /locus_tag="VF226_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7074449.1"
                     /translation="MSSILLPEPNPDILARRDAIIADLKALVGADSVVSDEDGRRAFE
                     TDGLTAYRKMPLAVVLPQSTEEVSAVLRYCHQNGVKVVARGAGTSLCGGALPAEDAVV
                     VGVSKMNRVLDVDYASRTAVVQTGITNLAISGAVGKDGFFYAPDPSSQLACTLAGNLA
                     MNSGGAHCLKYGVTTNNVLGVKMVLMNGEIVEIGGQHLDAPGYDLLGFIIGSEGQFGI
                     TTEATVRILRAAEHARPMLLGFASTEDAGACVSAIIGSGIIPVAIEYMDKPAIEVCEA
                     FAKAGYPLDVEALLIVEVEGSETEIDDLLARLVKIAEPFKPKSMKVSSSPAESAKIWR
                     GRKSAFGAIGRISEYYCMDGTIPLKQLPRVLTRISEICGSHGLRVANIFHAGDGNLHP
                     LILYNANDPREVEKAELAGADILKLCVEVGGCLTGEHGVGVEKRELMRHQFSETDLLV
                     QMRIKSVFDPDWLLNPAKVFPLAEREMLENAA"
     gene            21904..22284
                     /locus_tag="VF226_10460"
     CDS             21904..22284
                     /locus_tag="VF226_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX7074450.1"
                     /translation="MNAKTLVGLLFLAALVSPAVADDAENGEQVFRKCRACHQVGDSA
                     KNAVGPQLNGIVGRQAATIEGFNYSAAMKEAGEKGLVWNEENLSKYLENPRKFIPKNK
                     MAFAGLKDDDDRKDVIAYLKKFSD"
     gene            22419..23192
                     /locus_tag="VF226_10465"
     CDS             22419..23192
                     /locus_tag="VF226_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX7074451.1"
                     /translation="MSSILPGAPRVGLFVTCLVDLMRPSVGFAAVKLLEQAGCSVEVP
                     LAQTCCGQPAYNSGDKADAAELARQTIAAFAGFDYVVAPSGSCAGMLKLHYPRLLAND
                     AAWVGRAKEFAERVHELVSFLVDVRGMDAVDATFQGRATYHDSCSGLRELGIKDQPRR
                     LLSSVKGLQLSEMADAEVCCGFGGTFAVKYPDISNAIVEKKTANTAAVEPDLLLAGDL
                     GCLMNIAGKLKREGRNIAVRHVAEVLAGETSHPPIAGTK"
     gene            23227..24654
                     /locus_tag="VF226_10470"
     CDS             23227..24654
                     /locus_tag="VF226_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX7074452.1"
                     /translation="MQPTTQSFKQNARVALSDAQLQRALAGLPTGLVANRAAARARLP
                     EFEDLRDIGRDIKDHTLAHLDLYLEEYERNATAAGAKVHWAATAAEARDIILRICREV
                     NAKIVTKGKSMISEEIGLNDELQSAGMEVVETDLGEYLVQIRGETPSHIIAPAIHLTQ
                     DQVEADFRRLHTKLPKDRVLTDPAQLVGEARQVLREKFVAADVGITGANFLIAETGSS
                     VIVTNEGNGDLTQSLARVHIALASIEKIVPTREDVSSILRLLARSATGQEFSTYTTFS
                     TGPRRPEDPDGPEAYHVVILDNGRSEMLGTAFREVLRCIRCGACMNHCPVYGAIGGHA
                     YGWVYPGPIGSVLTPSLIGVRDAADLPNASTFCGRCEEVCPMKIPLTKLMRHHREAEF
                     ELGGPSARYRLGLKAWAWLAKRPRLYHTTMRLVAAVLGGLGRRRGAFRSLPFAGGWTR
                     HRDFPAPQGRTFQQLWAEHKAGAPR"
     gene            24651..25340
                     /locus_tag="VF226_10475"
     CDS             24651..25340
                     /locus_tag="VF226_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HEX7074453.1"
                     /translation="MTSRDLILSKIRAGLGAKANDAERQAIVQNRIAAKARHLIPARV
                     TNRTPGELLALFRGFLERESATVLELDSKQEIPSAIANYLRSNNLGARIRVGGDPTLT
                     QLPWGSEPHLEMLLGRAQPSDEVGLTQAAAAVAETGTLVMASGPDNPVTLNYLPETHI
                     VVVEQRNLVPAYEDAWDVIRQRFGPQSMPRTVNFISGPSRTGDIGGRLVMGAHGPRRM
                     CVIVVRDPATS"
     gene            complement(25783..27165)
                     /locus_tag="VF226_10480"
     CDS             complement(25783..27165)
                     /locus_tag="VF226_10480"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEX7074454.1"
                     /translation="MSTRQDGAPSPTVRSRDDLVEWIAEGSKSKDKWRIGTEHEKFVF
                     YTDTLGPVPYEGPRGIRALMEALIDRYGWEPIMEGENIIALKRPKGEGAETISLEPGG
                     QFELSGAPVATLHETCAEAHTHLEQVSEVGKQLGLGFLGLGFSPKWTLEETPRMPKQR
                     YAVMTRYMPTVGRHGLDMMYRTCTIQVNLDFGDEADMVKKFRVSLALQPIATALFAAS
                     PFKEGKPNGFKSLRSEVWRDTDPNRTGMLPFVFEPGMGFERYTDYALGVPMYFVYRDG
                     RYIDVAGASFKDFMEGRLPQLPGERPTLDDWSDHLTTLFPEVRMKRFLETRGADGGRW
                     RRICALPAFWVGLLYDETALDAAWDLVKTWTAEERQQLRDTVPVEALATKFRSGTVQD
                     IAIEAVAIARTGLKNRNRLNSKQQDETIHLAFVEETAASGRTPADDLLARYHGPWQQD
                     INHVFTDHAF"
     gene            complement(27296..>27863)
                     /locus_tag="VF226_10485"
     CDS             complement(27296..>27863)
                     /locus_tag="VF226_10485"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEX7074455.1"
                     /translation="AVEGKRSCTLRPLEQVREQEAGPDIHYLFAPLKRARLDYMVQKA
                     TELGVAALRPVLTRHTVAERVNLGRMRSNVIEAAEQCGILRIPEIAEPEKLDRVLARW
                     NGDRPLVFCNERAPIQSPIEALGALRPGPIGVLIGPEGGFSAEEREQLIALPFVVQVS
                     LGPRIMRADTAAVAILALVNAVLGDWRG"
BASE COUNT         5037 a         9045 c         8805 g         4976 t
ORIGIN      
        1 cgaaacgtag tctccgacgt tcacccgcgg cgcaaaaggc gcgatggcca cgcgcgatgt
       61 atattcgtcc tgatttgccc aaacgacgat gttgatcagg tccttggctg cgagcttgag
      121 gtcctggatc ttgctgccgg acatcgaccc ggtgatgtcg agcatcatgc tgatttccag
      181 gtttgtctcg gcatttccgc cgacggccaa aacggccttg gcggatgtac gaaccggcat
      241 gctcttgatg ccgacggcgc tgaggaatgg cgtcgtgacg ttgccgacgg tcgaagctac
      301 gatggcggag ccgtcatccg ccaattcgaa gtggacgctc tcgtcattgg tcgcgtcggg
      361 cttcatttcc tcgtaatacg tgcgcgctgc ctgaagggcg gcttcggtat tgccaccgga
      421 cgcttgcagg acgcgccccg cagcgaggac ggctgcatcc accgcgttct gtgtctgtgt
      481 ccgggctgtc agccaccggc catagtcgat cgcgccgccg accgcaccga agatgaccgc
      541 gatcgtcagg ccgaagatca tggcgatatt gcccgactcg gatgagatga aagcgctgaa
      601 taccccgccg cccctgcggc ccggcgaccg ggaagggccg ttttcgaaca aacgcgttaa
      661 cttgatgaaa tcacgtccgc ttctagaatt ttgccccgag ccgaacatga atgcactcca
      721 acatctgcag gtctttcgct gctagccggt tggatgcttt cgaggcatta acggaacgca
      781 taaaatttta agcaacaagc agaacatagg gcgagagttt cattaccctt aactctcgga
      841 gcggaatagt accaatttgg ttcaaatgcc acccacgggc gagctgagag acaggtggat
      901 ccaactctcg ttcttgctcc gaccctagcg agttgctccc aggtatccga gcgactgcag
      961 gtaacggtag atctcgtcgg cgagaatgtc ggcgggaacc gttcctgcct gcaaatgcag
     1021 ctctggtgac tgcggaggct catatcgcga cgaaatgccg gtaaagttct gaatctgccc
     1081 ggcgcgggct ttcctgtaga gcccctttac gtcgcgctcc tcgcaaatct cgagcggcgt
     1141 atcgacgaaa acctcgatga actctccgtt gtcgaacaac tcccgggcca tgcggcgctc
     1201 ggcctcgaac ggggaaatga acgatacgag cacgataagc cccgcatcga ccatcaacct
     1261 ggcgacatgc gctgcgcgac ggatgttctc gacgcggtcg gcatcagtga agccaagatc
     1321 gcggttcaga ccatggcggg tgttgtcgcc atcgaggatg aatgtatgcc ggccgtgggc
     1381 gtgcaatttc ttctcgacca ggttggcgat cgtggacttg cccgatccgg acagccccgt
     1441 gaaccagagg cagcatggcc tctggccttt gagctcggcc cgcgatgcct tgctgacgtc
     1501 gagtgcctgc cagtgcacgt tcgtcgcacg ccgcagtccg aactggatca tcccggcgcc
     1561 aacagtcaga ttgctcagcc tgtcgatcag gatgaaggcg ccggtctccc ggttctcctc
     1621 gtagggatcg aaggcaacgg gctcgtgcgt ttcgatgttg acgaacccga tctcattgag
     1681 cttcagcgtc ttcgccgcaa gcttctggaa ggtgttgacg tcttcctgat gcttgatctc
     1741 cgtgacactg gccgtcacgg tccgcgcgcc gatcttgagc aggtaaggcc ggcccggaat
     1801 gagcggctcg tcgttcatcc acacgatgtg tgccgcaaac tgatcggaga cctccggccg
     1861 ctcatccggc gcagcgatga gatcgccgcg cgagatgtcc acctcgtcgg tgagcgtcag
     1921 cgtcacggcg tctccggcaa cggcttccgc catcgtcccg tcgtatgtca cgatcgaacg
     1981 gacccgcgtc tcccggccgg aattcgcgat gataatgcga tcgccggctc gaactcgtcc
     2041 gctggcaatc gtgccggaat agccgcgaaa atcgtggttg ggcctgttca cccattgcac
     2101 cggcatgcgg aatggtttgc tcgcggcatc ggcttcgacc tccacccttt ccagatgctc
     2161 gagcagaacc ggaccgcgat accatggcat ttccggactg cgcgcgatga cgttatcgcc
     2221 aaagcgtgcc gagatgggga tggcgataag gctcttgaaa ttcagatgat cagcgaaagc
     2281 gcgatatgcc tcggtgatgc gattgaaaac accctcgtcg tattgcgcga gatcgatttt
     2341 gttgacggcg agcacgacgt gacgaatgcc gagcagagcc gcgatgcggg aatggcggcg
     2401 cgtctgctga agcacgcctt tgcgggcatc gacgaggatg atcgcgaggt cagccgtcga
     2461 ggcgccagtc gccatgttgc gcgtatactg ttcgtggccc ggcgtatccg caacgatgaa
     2521 ctttcgtttt tccgttgcaa agaaccgata ggcgacatcg atcgtgatgc cctgctcgcg
     2581 ctccgcctgc aggccgtcga ccagaagcgc caagtcgaga tcgtcacccg tcgttccatg
     2641 cttcttggaa tcacgggcga gtttcgcaac ctgatcctcg aatatcagac gcgtatcgta
     2701 gagcaggcga ccgatgaggg tcgatttgcc gtcatccaca ctgccacagg tgatgaagcg
     2761 gaggagcgtt ttgtcctctg cggaaccatc cccctggcgc aaacggcccg tgagatcgag
     2821 aggcacgtgt tgttgcatca gaagtatccc tcgcgcttct tcttttccat cgagcctgct
     2881 tcgtcgtggt cgatgaggcg gccggagcgc tcggatgatc gcgtgaccag catctcggcc
     2941 acgatttcgt ccagggtcgc cgcggtggaa gggatcgcgg cagtgagggg atagcacccc
     3001 agcgtgcgga accggacgct tcgcagctcg gggcgttccc cggtcgcaag cggcattcga
     3061 tcgtcgtcga ccatgatgag ctggccgtct cgcacgacaa ccggccgttc acgagcgaaa
     3121 tagagcggca cgatagggat gttttcagcc gcgatgtatt gccagatgtc cagctcggtc
     3181 cagttcgaca gcggaaacac gcggatcgat tcgccctggc ggaccctggt gttgtaaagg
     3241 cgccagagct ccgggcgctg gttctttgga tcccaggcat gcgttgccgt ccggaacgag
     3301 aagatgcgct ccttggcccg ggaggcttcc tcgtcccgcc gtgcgccgcc gaatgcggcg
     3361 tcgaagccgt acttcgtcag ggcttgcttc agcgcctcgg tcttcatgat gctcgtgtaa
     3421 agcgccgagc cgtgcgtgaa cggtgaaatg ccctgcgcca ggccggcctc attgacatgc
     3481 acgatgaggt cccatccgag ccgccgcgcg gtctcgtcac ggaacgtgat catctcccgg
     3541 aatttccagg tcgtgtcgat gtgcatcagc gggaaaggag gcttggacgg gtgaaacgcc
     3601 ttcatcgcca gatgcagcat caccgaggag tccttgccga tcgagtacag catgaccggg
     3661 ttccggaact ccgctgcaac ctcgcgcatg atgtaaatcg attcggcctc gagccgcttg
     3721 agatgcgagc tgcgcttggc catcgacagt gccccccttg gctgtgctac cccgtgcgcg
     3781 tcgcggcaag ctctacctcg tcagaagctt gatcgctaga gtacgccaca cgcatgcagt
     3841 gaattgcgcg acgtgctcac ccagcgcatg acgaaaagac ggtatgatta tggacaagaa
     3901 cggctcgggg cttccggcgg aggcgaaggc aatcatcgtc ggtggtggcg ttgtcgggtg
     3961 ctccgtggcc taccatctgg cgaaactcgg ttggcaggat gtcattctcc tcgagcgcaa
     4021 gcggctgacg tccggcacga catggcatgc cgcagggctc atcgggcagc ttcgggcgac
     4081 gctcaatctg accaagctcg caaagtactc ggcggagctt tacctgggcc tggaggcgga
     4141 gaccgggatc gcgatcggct tccggcagaa cggctcgctc ggagtggcgc tctcgaacga
     4201 tcggtacgag gagttcgcgc gtggcgcctc gatggccagg accttcgggc tcgaagcgca
     4261 catgctcacg cccgccgaat gccgggagaa atatccgctg ctcaacctca atggtgtcgt
     4321 cggcggcatc ttccttccaa cggacggcca ggctgatccg gccaacattg cgcttgcgct
     4381 cgccaagggt gcccgtcaaa gcggagtccg gattctcgag ggtgtcaggg tcaccggcat
     4441 caccaaggca aaaggccgcg tcaccggcgt cgtgacggag cacgggacga tccgtgccga
     4501 gtacgtcgtc aactgtgctg gcctgtgggg ccgcgaggtt ggccggatgg cgggtgtgaa
     4561 cgtgcccctg caggcgagcg agcatttcta cgtggtaacc gaagccaatt ccgccatccc
     4621 gaaggatctg ccggtgctgc gggtgccgga cgagtgcgcc tattacaagg aggatgccgg
     4681 caagctgctg gtcgggttct tcgagccggt ggcgaagccc tggggcatgg acggcgtccc
     4741 cgaggatgcc gagttcctca cgctgccgga tgactgggag cacatcgccc cgcagctcga
     4801 aaaggcgatg gagcgcgtgc cgctgctggc cgaaaccggc attcacacct tcttcaacgg
     4861 cccggagagc ttcacccccg acaatcgcta cctgctggga gaggcgcccg agctcagaaa
     4921 cttctacgtt gcagcaggct tcaactcgat cggcattcag tcggctggag gggccggcat
     4981 ggcgctcgcc cactggatgc aggacggcga gccgccattc gatctgggcg acgtcgacat
     5041 tcgccgcatg tttccattcc agggcaatcg gacgtatctc gtcaatcgcg tgaccgaaac
     5101 tttgggcctc ctctacgctg atcacttccc ctaccgccac tacgagtccg cgcgcggcgt
     5161 gcggcacctg ccgctccacg agcgccacgc cgcgttcggg gcctgcttcg gcgaagcagc
     5221 gggctgggag cggccgttct ggtatctgcc ggccgaagca cgtgctcgcg gggaaagacc
     5281 tgagtatcgc tatagctgga agcggcagaa ctggtttgcg tatgctgcag aggagcatgc
     5341 ggccgtgcgc aacggtgtcg gcctcttcga cctgacgccg ttcggcaaga tccgtgtcga
     5401 gggtcgcgat gcggaggcgg tgctgcagcg catatgcgcc aacgacgttg ccgtcgagcc
     5461 agggcgcatc gtctacacac aatggctgaa ccagaaaggg ggcatcgagg cggacctgac
     5521 ggtcacgcgg ctttcagaaa ccgagttcct ggtcgtgact tcgtcaggca cgctgccacg
     5581 cgacctcaac tggctgaagc ggcatattcc ggaggatgcg cattgcatcg cgacggacgt
     5641 gaccagcgcc gaggcctgcc tcgctgtgat gggtccgcgg tcgcgggagc tgctggcgcc
     5701 gatcgtcgat gccgacctgt cgaacgaggc gttcccgttc ggcacggcgc gcgacatcga
     5761 aatcggcatg gggctggcgc gagcgcaccg cgtcagctac gtgggcgagc tcggatggga
     5821 gctttatgtc cccaccgata tggcgcggca tgtcttcgat gtcatcgccg agcgagggag
     5881 cgcggtcggc ctgcgcctgt gcggcatgca cgccctcgac agctgccgga tcgagaaggc
     5941 cttccggcac ttcggccacg acatcagccc cgaggaccac gtgctcgaag cggggctcgg
     6001 ctttgccgtc aggactgaca agcccgacgg gcgcttcggt ccgttcattg gccgcgaggc
     6061 cgttctgcgc aagaaggagg cggggcttac gaagcggctg gtacagttcc agctcgtgga
     6121 tccggagccg ctgctatacc acaacgagcc gatattgagg gatgggaagg tcgtcggtca
     6181 tctgacgtca ggggcctatg gccatcacct cggcgcagcg attggccttg gctatgtgcc
     6241 gacatctccc caggagagcg cgcaggagct gctcgcctca cgctacgcca tcgacatcgc
     6301 cggcaagatc gtgcctgcgc gggcgagcct gaagccgctc ttcgatccga cgggtgcgcg
     6361 aatgcggggc taggctggac ggcggcggga gcggaccagg gccgagctga gcaagtcctg
     6421 gagtcgtgcc ccgtcttgtt cggtcagttt ggcgcgaatg gccagcgtgc ggcttctggt
     6481 tgcaagctcg gcaagtggtc caccgccgcc ccgaagacgg acccaatcct tttccgtcgt
     6541 caccggaatt gcgtcgcgag aagcggcaag atccaggatg cgtttcgcat cctttgcacc
     6601 aaacgcatga tgatcggcca gagcgacagt tgccacgaga tcgccgccaa gctcctggag
     6661 cagcgcgaag aaccgctgcg gattggcaat cccggcataa gcgacgagcc gggcgccctt
     6721 gatccaatcc aaatcgcctt gcggctcagg cgcagcgctg aggatggggc cggtgaagga
     6781 tccgagccaa tcgagattgg catcgcgagc tgctcccggc ggccggttga cgacgatggc
     6841 gtccagcatc gcgagctgga acgcgagccg ggcgcgcagt ggccctgccg gaagcacctt
     6901 tccgttacca atcccgcggt gggcatcgat gagcgcgagg gtgaggtcct tgacgagaga
     6961 cgggttctgc aggccatcat ccatgacgat caccgttgcg gacccggccg cttcgatggc
     7021 gcgcgccccc gccgcgcggt cccgtgatac cagcgtcggc gcgattctcg ttaggagtag
     7081 gggctcgtca ccgacatctg ctgccgtgtc cctttccggg tcgacccaat gcgggccgcg
     7141 gattcgcccc ccatagccac gcgtcaggag gaccggccgt tctcccaatc tggcgagcgt
     7201 ttcggcgaca tggatcgcaa acggcgtctt tcccgttccg ccgaccgtca ggtttccgat
     7261 gcagatgaca ggaagcgagg accgaaaagg cttcgtcttt cggaaccgcc gctcgacagc
     7321 ccaggcgtaa gccgcgctca cagggcgcag ggcgcgcacc accgccggcg ccgaccctgg
     7381 ccggtaccac cagccgggct cgtcaagagg cacgcctcag gccttccttg tggtgcagca
     7441 gaggcagcaa ggcctccacc gtccgccgaa gagcgccgga cagatcggat aacaccgcct
     7501 gggcggccgt ccgcatccgg accagctcca cttcgttatc gagaagcgat ttgacggcgt
     7561 gcgccaactc ctccgccgat gcgacctggc aagccgctcc acggcggatc agcgcagagt
     7621 acgcatcgcc aaaattgaac cagtgcggtc cggtgatcac gactgccccg tgacggatgg
     7681 cctcgatcgg gttctgtcca ccccgcgaca ccagtgatcc gcccacaaat gccacaggag
     7741 aaaggctgta gaacgtcccg agctcgccca gcgtgtccgc gacgtagatg tccgtcgcat
     7801 cgtccggcaa tgcgccacgc gagcgctgcg cgacgttgag gccctcgccc cgaagcaact
     7861 ccgcgattgc gcccccgcgc tccgggtgac gcggcacgag catggtgcag agccgcggga
     7921 agctctgttt gaggatcttg tgcgccgccc cgatcgcttc gtcttcgcct tcgtgcgtgc
     7981 tcgcggcgat gaagcagggg cgtccggcca gcgcatcgcg aagccgccgc acctccaccg
     8041 gatcggcctc tggagccggg gcgtcgatct tcaagttgcc gacgaactgg acgttgcggg
     8101 cgccaagctc ccggaacctg cgggccagct tcccattctg ggcgatcacc aggtcgaaac
     8161 ggctgaacag cggccgtgcc attcccttgt tcttgcgcca gcggtcaaag gaacggttcg
     8221 acatgcgcgc attgatgagt gcgagcggaa taccgcgggc cgcggactcc aggatgaggt
     8281 tcggccagat ttccgactcg gtgaagacgg cgagctgtgg tcgccagtga tcgaggaacc
     8341 gggccacgac gtccggcgcg tccagcggca cgaactggtg cacgtcctca gggccaagcc
     8401 gccgcgccgc cacctcggcc gacgtcaccg taccggttgt aaggaggaag cggatatccg
     8461 gccgctccgc gcgtagagcc ttgagcagcg gcaggactgc gttcgtctcg ccgacgctcg
     8521 cggcgtgaat ccagataagg caaccatccg gacgcgccgc actcggcttg ccgaggcgct
     8581 cacctctccg cgcctcgtcc tctttgccgc gcctcgcccg ggcatcgagt atgagcggca
     8641 gcgccgggcg tgccaggctc gtcaacgcgc gataggcttt caacgaaagc cccggttgga
     8701 cgggtggtgc gacttcaccc ggcaacaatg gtgttgcgcg ggttggatcg gcgcccgcca
     8761 gctcgtacgc gcgccgggtc acggcattga gtccgtcctg cacacggatg cgcgcccgct
     8821 ccaggctttc cctgtcatcg tgcctgccca catgaatcgg ctcgccggca accacggcaa
     8881 gcgtaccaaa gggcaggttg agcgtcatac ggctccacgt gtcgaaggcc ttgtagcggg
     8941 atgtggcaac cgcggccggg atgatcgggc gtccggacag gcgcgccagc gtgacgatgc
     9001 cctcacctgc gattcgcgcc ggacccggcg gcacgtcggc cgtcatggcg acgcagtcgc
     9061 cgtcttccag cgcccgcaac gccgccctca gcgcctgggc accgccgcga tcgcgccggc
     9121 gatagccggc gccggcaccc cgaatcatgt ccatcttgaa atagtcgaga acctggccga
     9181 tcagctccgc atcgccatgg cgcgccacca tgctgcgcac attcagccca ccccgctcga
     9241 cccgcggcag catcaggaac tggccgtgcc acattgcgag gataaagggg ctgtgcaacc
     9301 tgacgtaact aactatgtcg gaaggctcgt tgacgatgcg cgacgtgcgg tggacgaaat
     9361 cgacatagtg cgcgatggcc cggccagcga ggcggttgag gcgagccttg tcgacggaaa
     9421 atagacccac tctttggcgc gccccttgcc cgttccgcga gaaattcgtg agagtaccca
     9481 tccacttgat tgaggtgtct atagcggata tgcagccttt gcaaatgcga ttctatcgaa
     9541 ctggcgcccc cgagttgcgt gcctgcgagc aattctcacc gaaaacagac gaatggcgaa
     9601 tctgagcctg gcgaagcgca cccggcggcg ggtcaatcgg ctggtaagca gttgcaggca
     9661 cacgctcgtt gagagcagcc ctggctgggc gaaaaccgcc ttcgggccgg cggcaagcta
     9721 cttcgacatg ctgttcgtcg accacggcat attccggttg ttctacctca acagacaccg
     9781 gctcggcgag caagcatggc gatccgcaca acccgcgccc catcaaatca gggctatggc
     9841 gcgggacggg atccgcacga tcgtcaatct gcgtggcgag cacccctgcg gtagctactg
     9901 gctggaggaa gaagcctgcc gccgctacgg catcgcgctc gtgaattttc gggtccgatc
     9961 ccgcgccgct ccaagccgta gtgagctgcg aggcgcccgc gagctgttcg agcggatcga
    10021 gtacccgatg ctcatgcatt gcaagtccgg cgcggacaga gccgggctga tgagcgtgct
    10081 ttacaggcat ttccgcgaag gtgtgccgct cgaaatcgca aagaaggagc tgtccttgcg
    10141 tttcggacat atccggcagg cggacaccgg tgttctggac tacttcttcg accgctatct
    10201 tcaggacacg gctgagcggc cgatgccgtt cctggagtgg gtcgacaagg tctatgaccc
    10261 gaaggagctg aagcgcacat tcatggccaa gggctgggcg aacaagctcg tcaacgacat
    10321 cctccgcaga gagtagcgag cctcggcggg agtgccgtgt acgggttccg ccctcaacga
    10381 cttgatcagt gggacggatt ggagccggga atgggtgccg gatcggggtc cagcagccgg
    10441 tgcaggtgga cgatgaagta gcgcatgtgg gcgttgtcca ccgtgcgctg ggctgccgcc
    10501 ttccacgcct tgtaggcctc ggcgtagttc ggatagatgc cgacgatgtc gaggtcgtcg
    10561 aggttgcgaa actccacgct gtcgagagag accagctcgc ctccaaagac aaggtggaga
    10621 agctgcttct tcggcggctt ccgcgtcgga tcggctctgc tcacgatcgg cctccgtcat
    10681 catgaattcg cagcccgcgc tggcatgggc cggtttacct gagtctgaac ggacccgcac
    10741 gctctgccat gctacgagag cttgggggtc agtgccggct gaacacctcc gtcttcccgt
    10801 cccggcgaac gagcagaact tcaaagggct ccttctcgtt gcctgcttcc atcccaggcg
    10861 agccgagcgg catacccgga acggtcagcc cgatgacgtc cggcttctcc gccagcagcc
    10921 gccgaatgtc ttccgcgggc acgtgtccct cgatcacata gcccccgatc ttggccgtgt
    10981 gacacgactg cagatccggc gacagaccgg cgtcggcctt gacacgggcg agctgcccgg
    11041 ccgccaagtt ctgggccttg gccgaaaaac cattctgctc gatatgcgcg atccagccag
    11101 agcagcatcc gcaggtcgcc gacttgaaga cctcgatcgt ctgtccggac gcggttgcgc
    11161 caaggccgaa tgccagaccg gcaacggcca cgatggttcc tacaagggag cggatcgtca
    11221 taatctcttg cctccagctt cgaattcaat ctggcgccca cctgacgatt tcgccagccg
    11281 ccgcaccgta ggcaccctcg catcagcacc caaactcacg ggccacgcag gcggccggtg
    11341 ggcccgtttc atggaacaac tcgctgaact tcggcttgac gccgcacaag cggctagccc
    11401 gatttccgcc ttcttgcact ccggttgccc tgcggcaata gagcacccag cggcatgttc
    11461 aagagcctgc agcgttgtca aagctcgtgg gctcgttgag tgacccgaca agataattgc
    11521 tcgcgggcgt ttcatgaggt gccgcatggc acatcatcgc gtgtcgccct ctacagcacc
    11581 ggcgggcgag gatgcctctc ccaaacagct agtcggcctg accttcggct cgatcggcgt
    11641 cgtttacgga gacatcggta cgggcccgct ctacgccttt cgagaggcga cgcaggccac
    11701 cgtttcagcg gggttgccac cgcaagaggc cgtgctcggg gtgctttcac tcattctctg
    11761 ggcgctgttc gtcatcgtca cgctcaagta cgtcttcatt ctgctgcggc tcgactatca
    11821 cggcgagggc ggcaccttcg ccctgatggc ccttggccaa tccgcggctg ggaaaggtgc
    11881 ctatgcgatc ctcgttctcg gggttgcggg cgcgtcgttc ttcttcgggg atgccgtgct
    11941 gacgccggcc atctcggtgc tctccgcggt cgaaggcgcg aagctcatcg cgcccgcgct
    12001 cgacaacgtc gtcctgccgg tcacgattgc gatcatctcc ggattgttct ggttccaatc
    12061 ccgcggcacc gcgagcgttg cccggttctt cggaccggcg atggcgatct ggttcgccct
    12121 gctcgcgctc ggtggcctca agcatatcgt ggacgatccg cacgtcttcc tcgcgctgaa
    12181 tccgttctac gccatcacat tcgttgcctc tcatggcatg acgagcctcg cggtgctggg
    12241 gctggtgttc ctcgccgtga ccggagcaga ggccctctac gccgacctcg gtcacttcgg
    12301 acgcaagccg atccagcatg catggctagg cctggtcctc cccgcgcttg ccttgaatta
    12361 ccttgggcag ggggcgcttg tcctggccag gcccgaagcc atcgccaatc cgttctatct
    12421 gttgtacccg agctgggcgc tggcaccgat ggtcgtggcg gccacgctgg cgaccgtgat
    12481 cgcgagccag gccgtcataa ccggcgccta ttccatgacc cgccaggcgg tacagctcaa
    12541 gctgctgccg cggttcgaca tccgccatac ctcagagacc gtggccggcc agatctatct
    12601 gccgcgcatc aatgtcctgc tgttcatcgg cgtcgtgctc gtcgtgatgc ttttccggtc
    12661 ttcgagcaat ctggcatcag cttatggcgt ttcggtctcg gccgagatgg tcatcacgtc
    12721 gagcatcgcc tttttcgtca tctgttacct ttggcgatgg ccgcgctggc aagcggctgc
    12781 cctcatcggg ccactgctgc tcatcgagct cgtattcttc ggctcgaaca tgttgaaggt
    12841 gctcgaaggc gggtggctgc ccctgctgat cgcatcggcc gtcgccaccg tgatgttcac
    12901 ctggatcagg ggcgctcgcc tgatcataga aaccctgcgc aagactgaaa cagatctcga
    12961 ttggctcgtt cgcaaactgg aggcgaagcc gccccaccgc gtgcccggaa ctgccgtatt
    13021 cctgacggca ggcgcgaacc tggcgcccat atcgctactt cacaatctga agcacaatcg
    13081 cgttctgcac cggcgcaaca tcatcctgac catcaggagc gaggaggtgc cccgggtgcc
    13141 ggcgtccgag cgcatcacca tcgagccagt gggcgacggg ttcatccgtg tcatcgcgcg
    13201 atacggcttc atgcagacgc cgagcattcc ccgaatcttc gcggcgtgcg agcggaaagg
    13261 gttgaatgtc gatgtcgcga cggcgtcgta cttcctgtcc cggcgctcac tgaagccgaa
    13321 aagaaagtcc aagatgcccc actggcagca aatcctgttc atcaggttgg cgcacacggc
    13381 acaggatgct acgggctact tcaaaatccc tacggatcgg gtggtcgaga tcggcgcgca
    13441 ggtggctgtc tgatcaggtt gcctggacgc cggcaccacg atcgtcccgc ttctgccgcc
    13501 ggcggtacca gaagctcatg atctccatgg aacctgccgt aaggagccca gccgcaagca
    13561 agaccgcctc ggcgaccgtg tagccccaca ggtcgacgac aacagccagc acggccgggg
    13621 ctatggcgtt cacgagcaga tacggcgttg cgagaatacc gagcaccgcc ccgtagccct
    13681 gcgggccaaa gatggccaac ggcacggcac cgcgcacgat cgtcaccagt ccattggcaa
    13741 cgccgaacag tatgatgaag agcagggccg ccgaggtgct ggcgcctcct gccatcaacg
    13801 cgatgaaggc cagtggcatg ccggccaccg aaatgcgccc aaggtcgatg gcatggaggc
    13861 gcttaccaaa caggatctcc gccacgcggc cggcaacctg cgcgaccccc ttgaacgagg
    13921 cgagcgtcac tgctgccgcg gcggacagcc cgttggcctc cagaatggcc ggcagatgca
    13981 ctgccatcgc accgaatacg aaggcgctgg cagacatgac gagcccgaat agcaccatgg
    14041 ccagggtgcg cgcttgtcca tcgagcggcg gatcagacat cccggctgcc gcagaggccg
    14101 gcgcaccgct gatcgcggca gcgggtgccg gcggctcgcg ccgggccagg ccccaccaat
    14161 gcagcggcag gcagaccgcc aaattgaacc cggcgtagat gaccagcgcc atccgccagc
    14221 ccacctccgc cgtcagtatg tgaccgatcg gccagaaaaa cgtcgaggcg aagccgccga
    14281 agagggtgag gtaggagatg gctcgtcgcc cgcggtgcgg cgcgacctgt accagtgcgg
    14341 cgaaggcggc atcgtagagc gacatgcgca tgccgacgcc catcgcggcc cagccggcga
    14401 ggtagacgat cggattgctg gcagcggcaa cgatcagcag cgatatggca cacaggatcg
    14461 agcctacgct catgacgagc cgcccgccct gggtgtcgat cgccttgccc actgcggtgg
    14521 agacgaagct cgacgccagc agaccgatgg tgagccccga gaagacgagg ctgcggctcc
    14581 agcccgtgtc ctcggcgata ggactggcca ggacgccgag cgcgtagagt gtcgtgcccc
    14641 aagcgacgat ctgcgtgata cccagcgcga cgatggccgt gccgatcggc gatacgagcc
    14701 agactaccca ggcccactgt gctggcccac ctttgacgcg ctgcgacatg cctcccttct
    14761 ctcctcccgg atcggctcgc gtgtcacatt agcttctcgc cggcaggctc gccatcgaca
    14821 cggaatgcaa ggctcgtctg cggcattgcc ggtcaggcag gcatctggac ggggcagcgg
    14881 gcagagccaa tatcctccgc agaggagatt cggcagaatg aacgacagcg aagagaaccg
    14941 ctttgcggcc cgtgcggcgc gctatgtgcg ggtggggacg aacgtgggcg ccgtcgcagc
    15001 ccgggtcgct ggacagcggc tcttgggcat ccggggggac cgctccaaaa acgccatgga
    15061 cctggcggtg gctctcggcg gtctcaaggg accgctgatg aaggtggcgc agctcctgtc
    15121 caccatccct gaagccctgc cgccagagta cgcccgcgag ctgtcacagc ttcaaagcca
    15181 ggcgccgccc atggggccgg cctttgtgcg ccgccgcatg atggccgagc tcggccccga
    15241 ctgggcatcg cggttcaaga gcttcgagac gacagcggca gcctcggcct cactcggtca
    15301 ggtgcacaag gccatttctc atgacggccg cgagctcgcc tgcaagctgc agtatccgga
    15361 tatgcagtct gcggtggaag ccgacctcaa ccagctcaag atcatttttt cgatccacgc
    15421 gcggatggat cccgcggtgg atacccgcga gatcatgacc gaaatcgccg cgcgcctgcg
    15481 ggaggagctc gactatgagc tcgaggcgcg gcacatgcgc ctctatgccc acatcttcga
    15541 aaacgatccg ctgatccgcg tgccgttggt cgagagcgag ctgtcgacga agcgcctcct
    15601 gaccatgacc tggctcgagg gcaagccact gctttcctac aaggaagcgc ccctcgagca
    15661 ccgcaatacg atcgcgcgcg ccatgttccg cgcgtggtgg tatccgttca gccactacgg
    15721 cgtcatccat ggcgacccgc acctcggcaa ctataccatc ttcgagcagg acggtgcgcc
    15781 ggccggcatc aatctgctgg actatggctg cattcggaca ttccgcacgc cgttcgtgca
    15841 gggcgtgatc gacctctaca cgggtctgct cacggggaac caggacctcg tcgtgcacgc
    15901 ctacgagacc tgggggttca aagggctcac gaacgagctg atcgaggcca tgaacatctg
    15961 ggcacggttt atttacggcc cgctgctcga cgatcgcgag cggaccattg ccgaaggcac
    16021 cacacccggc gagtacggcc gccgcgaagc cttccaggtg cacaaggttc tgcgggagaa
    16081 aggtcctgtc gtcgtgccgc gcgaattcgt cttcatggac cgggcggcga tcgggctagg
    16141 cggcgtcttc cttcacctcg acgcccgcat gaactggtac cgcctgttca acgagacgat
    16201 cgaggacttt gacctggcca gcgtcgccga gcgccagaaa accgcattca ccgcagccgg
    16261 agtcccgttg ccctgaaccc gaacgaattg gcgaaggcga gccttaccgc ggccgcctcc
    16321 gctgcggcgg gcgctcgcga tggacgggcc ttaccggagg atgcccaaac ggccggcccg
    16381 gcttggcgta catctgcgcc gacggcaccg agacgacctc gcgctccggc cagcgaagtg
    16441 ccgtcagctt cccgccgaaa acgcaaccgg tatcgatgca aagcgtattg ttgacccaat
    16501 ccgcctgagg caccggcgtg tggccataaa cgatggtggt ttcgccccgg taattggccg
    16561 cccagtggta gcggaccacg aggcccgttt cgtccgtctc accatcggtg tcgccataga
    16621 ggcagaactc gcgaacggct tccgactccc ggccgatcat ttcctcaaga ataccgccat
    16681 gcgccacaac gagacgcccg tcctcgagcc agaggtggct cgggagactg agcagaaact
    16741 cccgcacttg cgcgcggaac cgtccgctct cctcggaaaa ctgtgccacg gtcttatcga
    16801 ggccatgtgt caggcggacg ttggcgccgc gcagccacct caagaatttc acgtcgtgat
    16861 tgcccggcac tgcgaaggct tggcccttct cgaccatgct catggcgatg cgcagcacgt
    16921 ccggtgaccg agggccgcga tccacgaaat caccgacgaa gatcaggcgc cggccgggcg
    16981 gagtttcgac ttcggcgcgc cggtcagggc caacgcccgt gatgcgcaac ttattatagc
    17041 cgagccgctg catcagctct gtgagctcat cggcgcagcc gtggacgtcg ccgatgatat
    17101 cgaacggacc gtgctcgtgc cgcagatccg gtggcaggcg ctcgcgcgtg atcgacaccg
    17161 cctcggcgtc ttggggagcg atcggatggg tcggtttgaa gccggtcgcc tccaggctgc
    17221 gcaccagccg aagatcggcg cggtctcccg gggtacagag cacgatggcc accggctgtg
    17281 cgtagaattt cttggcaagc cgcaagagat cgatctgctg ctgcgtcacc gtctcgcttg
    17341 cgttgatggc gaccagctcg cggcaggcca ggcgatctgc ggcgatcagt ggccaggttt
    17401 ccgagctgcg gtccaggatc tcctcggcag cgaagtgccg gcgcatgaat gcggtcgtgc
    17461 tctcagtgct ggcgccgacg acggcaacga ggcagaaatc cggaattgtg atcttcactg
    17521 caggcaaacc gttcggacgg tggctatccg cgcaactctg ccaaaagggg gacggttcgc
    17581 cggcgggccc aagtgatcac acccgcagaa agtcctgaag cgagcgcttc gccatggcat
    17641 tctgcggctc aggaacgtcg ctgaccaggc tttcaaagcg ttcgagaccg cgcggcaccg
    17701 gaccgccgta ggcccagtcg agcagctcga tcgtatgcac gatcgggatg tccatcccgg
    17761 tggcgagctg ggtgatgcag ccgatgttcc ccgccgcgac gaggtcaggg cgcagggatt
    17821 cgatgtgtcc gactttgcga tcacgtagct gccgcgcgag atccggctgc aggatattgt
    17881 aggtgcccgc cgacccgcag cagatgtgcc cctccggtac gtcgaccaca gtgaaacctg
    17941 catcgcggag gagctgcttg ggctcctccg tcacacgctg gccgtgctgg agcgagcagg
    18001 cggagtgata ggcgacgcgc agcgacgacc agcgcttcgg cgggccgaga tcatactcgg
    18061 acaggaactc cgtcacgtcg cgcgtcatat cggcaatctt gcgggcgcgc tcggcatagc
    18121 cttcaacacg ggccagcagg tgaccgtaat ccttgaccat cgtgccgcaa cccgaggcat
    18181 tgatgatcag cgcatcgatc cgatcgcggc tcatcacctt ccaccaagcg tcgacattcc
    18241 gcttggcgaa ctcgaccgcc tcggcctcgc gccccatgtg atgcaccagc gcaccgcagc
    18301 agcccgcgcc tggggcgacc tccacgtcga cgccgcggcg ggcgagcagg cggatggtgg
    18361 cgtcgttgat ctcgggccgc agcacctgtt gagcgcagcc cgccagcatg atcacgcgcc
    18421 ccttgcgctc tgccttcgtc acggccgtgc cggggccgct gtacttgggc gagcgaagcg
    18481 accggctcgg cgccaggtcc agcatggcca ccagctcctt cagaccgagc cgtcgcatga
    18541 gaccacgcag cggcaggccc agtggcgcca gccggaggac ggcacggaac cggtcgggat
    18601 aaggcaccac ctccgccagc agctttcgca tcagccgctg cttgagcgag cgctggccgg
    18661 tgttctcgat atgcgcgcgg gcgtggtcga caagatgcat gtagtcgacc ccgccggggc
    18721 aggtcgtcat gcaggaaagg cacgagaggc agcggtcgac gtgctgctgg acctcggggc
    18781 tcgccggcac cccgcgctcg aacatgtcct tcatcatgta gatgcggccg cgcggcgagt
    18841 cgcgctcgtc cccgagcagc acgtaggtcg ggcagacggc ggtgcacagg ccacagtgca
    18901 cgcagcgccg cacgatgcga tcgatctcct ctatgcgtgg atcttcgagc tgctcggccg
    18961 tgaaattcgt ctgcatggtc tttctgcccg cctttggcgg aatgagttca gatcgcggcg
    19021 tacatccggc ccggattgag aatgcctgca gggtcgaacg ccgacttgat gccgcgggtc
    19081 aaccgctcga cgccgggctc gagcggctgg aagacgtcga tcgcagagcg gaccgccggc
    19141 tcggcgcgca tcaatgttgc atggccgccg aattgggcga tgacccggcg aatatctgag
    19201 gcgcctgcat ccgccgcctc gggcaactcg agccagatca gcccgcccga ccaatcatag
    19261 aacgcatcga ccggcatgta acgtctgatc gcctcgacga ccctgtagcc attggtcggc
    19321 ttcgtcgaga tacgccaaag cggccttgaa tcctgctgca gaaccgaaag ccggcgcagc
    19381 tctccccaga acgcgcgcga cacctcgtca cccagcacct ccgccttgcc ataaggcgcc
    19441 agattttcac gcagcttgtc gcagcggtac gcgacgaagc tcgcaaggtt ttcgatcctg
    19501 agtgccgtca cggccttgcc gggctcgcgg aattcgggcg tcaggaggcg ttgcgccatc
    19561 ggctgcggca gatgcacggc accgctcacc tcgtaaggcg tgcccatggc ctggcacatg
    19621 acctcggtgg cgagatcatc ggtcaatccg aacagcgcga cagttgccgt ccgttccggc
    19681 ctcgggacaa ccttgaacgt cacctcggtc agaacagcga gcgtgcccca gctcccggcc
    19741 accccgcggg cgacgtcata tccggtgaca ttcttcaaca cccgtccgcc cgacttgaac
    19801 gcctcgccgc gaccgttgat ggcgtgaaca ccgagcagat ggtctcgtgc cgagccggtc
    19861 gccacccgac gcgcgcctga tagattggtg gcaaacaccg ctccgatcgt gccccgtccg
    19921 gcctgtccgc cgagcgcggg tccgagatcg atgggctcga acggcagcat ctgccccttt
    19981 tcggcaagct gtgactctat gtcggacacc agcgtgccgg cccgcgccga catcaccagc
    20041 tcggtcggct cgtaaagcgt gacaccgcgc agaccggctg ttgagatggt cacagccgcc
    20101 tgcaccgggc ggccgacgcc gcgcttggtc ccggcgccca tgatctcgac gggcttgccc
    20161 cgctcggcag cggcgagaat gagattcgac agctcccact cggcggcagg tctgaccgtg
    20221 gccacggctc actagctcct cacgccgcgt tttccaacat ctcccgctcg gcaagcggaa
    20281 aaaccttcgc cgggttgagc agccagtccg gatcgaacac ggacttgatg cgcatctgca
    20341 cgaggaggtc cgtctcgctg aactgatgcc gcatcagctc gcgcttttcg acgccgacgc
    20401 cgtgctcacc cgtcaggcat ccgccgacct cgacgcaaag cttgaggatg tcggcaccgg
    20461 ccagctccgc cttctccacc tctctcgggt cgttcgcatt gtagaggatg agcggatgca
    20521 gattgccgtc gcccgcgtgg aagatgttcg caaccctcag gccgtgactg ccgcagattt
    20581 cggagatccg cgtcagcacg cggggaagct gcttcaacgg aatcgtcccg tccatgcaat
    20641 agtactcgga aatgcggccg atggcgccga acgccgattt gcggcctctc cagatcttgg
    20701 cgctttccgc aggactgctg ctcaccttca tcgacttcgg cttgaagggc tcggcaatct
    20761 tcacgaggcg agcgagcagg tcgtcgattt ccgtctcgga gccttcgacc tcgacgatca
    20821 acaaagcctc gacgtcgagc ggatagccgg ccttggcgaa tgcctcgcaa acctcgatgg
    20881 ccggcttgtc catgtactcg atggcgaccg gaatgatccc tgaaccgatg atggcggaga
    20941 cgcaggcgcc ggcgtcctcc gtcgatgcaa acccgagcag catgggccgg gcatgctccg
    21001 ccgcccgcaa gatgcgcacc gttgcctcgg tggtgatgcc gaactggcct tcggagccga
    21061 tgatgaagcc gagcagatcg tagccgggcg catcgaggtg ctgcccgccg atctcgacga
    21121 tctcgccgtt catcagcacc atcttgacgc cgagcacatt gttcgtagtg acgccgtact
    21181 tgaggcagtg ggcgccgccc gagttcatgg cgagattacc cgccaaggtg caggcgagct
    21241 ggctcgaagg atctggcgcg tagaagaagc catccttgcc caccgcgccc gaaatcgcga
    21301 gattggtgat cccggtctgc acgaccgccg tgcggctggc ataatcgacg tcgagcaccc
    21361 ggttcatctt cgatacgccg acgacgacgg catcctccgc cggcaatgcg ccgccacaca
    21421 gcgacgtgcc ggccccgcgt gccaccacct tgacgccgtt ctggtgacaa tagcgcagga
    21481 ccgcgctgac ctcctcggtc gactgaggca gcacgacggc gaggggcatc ttgcgatagg
    21541 ccgtcagtcc gtcggtctcg aacgcgcgac ggccgtcctc gtcgctgacg acactatcgg
    21601 cgccgaccag cgccttgagg tcggcaatga tcgcgtcgcg gcgggccagg atgtccggat
    21661 tgggctccgg cagcagtatg gacgacatgt tcgcctcgag tctgttctgg cagcactttt
    21721 gcgatctctg ccatcagtag gccgttttcg cacggtcccg gccgcctggg caacctcgtc
    21781 gcgttgcgat cgagtgtcat ctgcgccatg agtcacgcaa cctgcatctt ttgcggcagc
    21841 gttgccgaat gtacaggttc acagtgctat catcggggct gactgatcgg aggggaactg
    21901 aaaatgaacg caaaaacatt ggtgggactg cttttcttgg ccgctctggt gagtccggct
    21961 gtcgcggatg acgcagaaaa cggagagcag gttttcagga aatgccgtgc ctgccatcag
    22021 gtcggcgata gcgccaagaa tgccgtcggg ccgcagctca acggcatcgt tggacgacag
    22081 gccgcaacca tcgagggctt caactactcc gccgccatga aggaggcagg cgaaaaaggt
    22141 ctggtgtgga acgaagaaaa cctttcgaag tacctcgaga accccaggaa gttcataccg
    22201 aagaacaaga tggcgttcgc tggcctcaag gacgacgatg atcgcaagga cgtcatcgcc
    22261 tacctcaaga agttttcgga ctagtatttt cctgctctgc cggtcggatt ggcttggcgc
    22321 gggttctcgg cgcgggtaca agcgaacccg agcccatgcg gtgcagatca cgggatctgc
    22381 accgcgcatc tcggttgaag ctgaaagcgt cattcttcat gagcagcata ctgcccggcg
    22441 cgcctcgtgt tggcctcttc gtcacttgcc tggtcgacct catgcgcccc tccgtcgggt
    22501 ttgccgcagt gaagctgctg gagcaggcgg gatgttccgt cgaggtgcct ctggcgcaga
    22561 cgtgctgcgg gcaaccggcc tacaactcgg gcgacaaggc ggatgccgcg gagctggctc
    22621 gtcagacgat cgctgcgttc gccgggttcg actatgtcgt cgcgccctcc ggctcctgtg
    22681 ccggaatgct gaagctccac tatccgaggc tgcttgccaa cgacgctgcg tgggtcggca
    22741 gggcgaaaga atttgccgag cgggtccacg agctcgtgtc ctttctcgtc gacgtgcgtg
    22801 gcatggacgc cgtcgacgcc acgtttcagg gtcgtgcaac ctatcacgac agctgctcgg
    22861 gcctgcgcga gcttggcatc aaggatcagc cacgccgcct gctttcctcg gtaaaaggct
    22921 tgcagctcag cgaaatggcc gatgcggagg tctgctgcgg cttcggcggc acgttcgccg
    22981 tcaagtaccc cgacatttcc aacgccatcg tcgagaagaa gacggccaac accgcagcgg
    23041 tggagcccga tctgcttctc gcaggcgatc tcggctgcct catgaacatt gccggaaaat
    23101 tgaagcgcga gggccgcaac attgcagtgc ggcatgtggc ggaggtgctg gccggagaaa
    23161 cgtcgcaccc gccgatcgcc ggaaccaagt agctagagcg cggcagccga cgtacaggat
    23221 cgcccgatgc aaccgaccac gcaatccttc aaacagaatg cgcgcgtcgc gctctccgat
    23281 gcgcagctcc agcgcgcgct cgccggcctg ccgacggggc tcgtcgccaa ccgcgcagcg
    23341 gcccgcgcac ggctgcccga gttcgaggat ctgcgcgaca tcgggcgtga catcaaggat
    23401 cacacgctcg cgcatctcga tctctacctg gaggagtacg agcggaacgc aaccgcagcc
    23461 ggagcgaaag tgcactgggc ggcgaccgct gccgaggccc gcgacatcat tctccgcatc
    23521 tgccgcgagg tgaatgccaa gatcgtcacc aagggcaagt ccatgatctc cgaggagatc
    23581 ggcctcaatg acgagctcca gtcggccggc atggaggtgg tcgagaccga tctcggcgag
    23641 tacctcgtgc agatccgggg cgagacgccg agccacatca tcgcgccggc catccacctg
    23701 acgcaggatc aggtcgaggc cgacttccgc cgcctgcata cgaagctgcc gaaggaccgt
    23761 gtcctgaccg acccggccca gctcgtgggc gaggcgcggc aggtgctgcg ggagaagttc
    23821 gtggccgccg acgtcggcat caccggtgcc aacttcctca tcgccgagac gggatcatcg
    23881 gtgatcgtca cgaacgaggg gaacggcgac ctgacgcaat cgctcgcgcg cgtgcacatc
    23941 gcgctcgcat cgatcgagaa gatcgtgccg acgcgtgagg acgtgagcag catcttgcgc
    24001 ctgttggcgc gctcggcaac agggcaggag ttctcgacct acacgacctt ctccaccggc
    24061 ccccggcggc cggaagatcc ggatggtccg gaggcctatc acgtcgtgat tctcgacaat
    24121 ggccgctcgg aaatgctggg caccgctttc cgcgaggttc tgcgctgcat ccggtgcggc
    24181 gcctgcatga accattgccc ggtttacggc gccatcggag ggcatgccta cggctgggtc
    24241 tatccaggtc cgatcggctc cgtgctgacg ccttcgctca tcggtgtgcg cgatgccgcc
    24301 gacctgccca acgcatcgac gttctgcggg cgatgcgaag aggtctgccc gatgaagatc
    24361 ccgctgacga agctcatgcg gcaccaccgc gaggcagagt tcgagctggg cggcccctcg
    24421 gcgcgctacc gccttggcct caaggcctgg gcttggttag ccaagcgacc gaggctctac
    24481 cacaccacca tgcggctcgt tgcggccgtt ctcggtggtc tgggccggcg gcgcggcgcc
    24541 ttccgctcgc tcccgtttgc aggcggctgg acgcggcacc gtgacttccc ggcgccgcag
    24601 ggccggacat tccagcagct ctgggcggag cacaaggcgg gagcgccgag atgacgagcc
    24661 gcgacctcat cttgtccaaa atccgcgcgg gtctcggtgc aaaggcaaac gatgccgagc
    24721 ggcaggccat cgtgcaaaat cgtattgccg ccaaagcccg ccatctcatc ccggcgcgtg
    24781 tcacgaaccg aacgcccggc gagctgctgg cccttttccg cggctttctc gagcgcgaat
    24841 ccgccaccgt tctcgagctg gattcgaagc aggagatccc gtcagcgatc gccaattacc
    24901 tgcggtcgaa caacctcggc gcccgcattc gtgtcggcgg cgatccgacg ctgacccagc
    24961 ttccgtgggg atcggagccg cacctcgaaa tgctgcttgg ccgcgcgcag ccatccgacg
    25021 aggtcgggct cacgcaagcc gcagcagccg ttgcggaaac aggcaccctg gtgatggcct
    25081 cggggccgga caatccggtc acgctcaact atctgccgga gacgcacatc gtcgtcgtcg
    25141 agcagcgcaa tctcgtaccg gcctatgaag atgcctggga cgtgatccgg caacgcttcg
    25201 ggcctcagtc catgccgcgc accgtgaact tcatatccgg gccatctcgc acaggcgaca
    25261 tcggtggacg gctcgttatg ggtgcgcacg gtccgcggcg catgtgcgtg attgtggtgc
    25321 gggacccggc gacgagttag tcatctgggg gaactgggtg gctatatcgc tactgatcca
    25381 ctctgaagcg tgatctaaac gtccctagct tcgcgggaat tgcggtgatg gggcaggacg
    25441 cgcgggctct ccaagctcaa cacccggagc gccttccact caccgattgc gtgtggcgat
    25501 gcaccgcctc atgcaggcgg cgaactgggc atcgcacgaa ggagatcctt tcgtggcgat
    25561 gcggcactgg ttgtattgcg cacgacagga tgccgtacac gagccccctt gggcgagctt
    25621 catctcggcg gccagcgagg gtaaacccag caaaccacct gacagcagcg ccagtatgag
    25681 gagcttgcga tacacctatg cacctcctgc cttgagtgca ggcgacttaa gctcgaatgc
    25741 gatgaataca gtctgaaccg tctcccctcc aggggcgaat gctcaaaagg catgatcggt
    25801 gaagacgtgg ttgatgtcct gctgccacgg cccatgatag cgcgcgagca gatcgtcggc
    25861 gggcgtgcga ccgctcgccg cggtttcctc tacgaaagct agatggatgg tctcatcctg
    25921 ctgcttggag ttgagccggt tgcgattctt gagccccgtg cgcgcgatcg cgacagcctc
    25981 gatggcaatg tcttgcacgg ttccgctccg gaacttcgtc gcgagggctt ccacaggcac
    26041 cgtatcccgc aactgctgcc gctcctccgc cgtccaggtc ttgacgaggt cccaggccgc
    26101 atcgagcgcc gtctcgtcat agagcaaccc gacccagaag gcgggcaacg cacaaatccg
    26161 ccgccagcgg ccaccgtccg caccacgcgt ttccaggaag cgcttcatgc ggacttccgg
    26221 gaagagcgtc gtcagatgat ccgaccagtc atcgagggtc ggccgctcgc ccgggagctg
    26281 gggtaagcga ccctccataa agtccttgaa cgaggcgccc gccacgtcga tgtagcggcc
    26341 gtcccggtag acgaagtaca tcggaacgcc gagggcatag tccgtgtagc gttcaaatcc
    26401 catgccgggc tcgaacacga agggcagcat gcccgtacgg ttggggtccg tgtcgcgcca
    26461 cacctccgac cgcagcgact tgaagccgtt gggctttccc tctttaaacg gtgaagcggc
    26521 gaacaaggcg gtcgcaatgg gctgcagcgc gagcgagacg cggaacttct tcaccatgtc
    26581 cgcctcgtcg ccgaagtcga ggttcacctg gatggtgcag gtccggtaca tcatgtcgag
    26641 gccgtgccgc cccacggtcg gcatgtagcg cgtcatcacc gcgtagcgct gtttcggcat
    26701 tctcggcgtt tcctcgagcg tccacttggg cgagaagcca aggcccagga atcccagccc
    26761 gagctgcttg ccgacctcgg aaacctgctc caggtgcgta tgcgcctcgg cacaggtctc
    26821 gtgcagcgtt gcgacggggg caccggaaag ctcgaattgc ccacccggct cgagcgagat
    26881 cgtctcggcg ccttcgccct tgggccgctt gagggcaatg atgttctcgc cctccatgat
    26941 cggctcccac ccgtaacgat cgatcagcgc ctccatgagc gcgcgaatgc cgcgcggccc
    27001 ctcgtatggc accggcccca gcgtatcggt gtagaagacg aacttctcgt gctcggtccc
    27061 gatccgccac ttgtccttcg acttggagcc ttccgcgatc cactccacga gatcatctcg
    27121 ggagcgaacg gtcgggctgg gtgctccatc ctggcgggtc gacatgaaga cctctgctct
    27181 agttcgaaag ttcgggattc cagaaatgct gctttatcgc cgccctcggg gtcgagcaca
    27241 agtgctcctt gcaggagggg agtgccaaca gggagccctg aggtgcccgt tcgggtcaac
    27301 ctcgccagtc gcccagaacc gcgttgacca acgccagtat ggcgactgcc gcggtgtcgg
    27361 cgcgcatgat ccggggtccg agcgagacct ggacgacgaa cggcaatgcg atgagttgct
    27421 cacgctcctc cgccgagaac ccgccctccg ggccgatgag cacgccgatg gggcccgggc
    27481 gtagcgctcc cagagcctcg atcgggctct ggatgggggc gcgctcgttg cagaagacga
    27541 gcggacgatc accgttccat cgggcgagca cccgatcgag cttctccggc tcggcgattt
    27601 ccggaatccg caggatgccg cactgttccg ccgcctcgat gacattgctg cgcatccttc
    27661 cgaggttgac gcgctcggcc actgtgtggc gtgtcaggac cggccgcagc gcggcgactc
    27721 caagctccgt cgccttctgg accatgtagt cgagccgcgc gcgcttcagc ggcgcgaaga
    27781 ggtaatggat gtccgggccg gcctcctgct cgcgcacctg ttccagcggc cgaagggtgc
    27841 agctccgttt gccttcgacc gca
//
LOCUS       DASVDU010000167        29871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1391936, whole genome shotgun sequence.
ACCESSION   DASVDU010000167 DASVDU010000000
VERSION     DASVDU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 29871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29871
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391936"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VF226_10490"
     CDS             complement(<1..528)
                     /locus_tag="VF226_10490"
                     /inference="COORDINATES: protein motif:HMM:NF006623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7074456.1"
                     /translation="MSMASVVGSHIPHLRRYARALTGSQAGGDAYVQQLLETLVVDPA
                     SLAKSSDARVALYKTFIQIITSVAASGWQPSPKALASGFDRNVAAMTPRSRQAFLLRH
                     VEGFTLPETAEILDASVEEVHELLEKAGEEIARQVATDALIIEDEPLIVMDLTQILEE
                     LGHRVIAVARTRDEAR"
     gene            791..955
                     /locus_tag="VF226_10495"
     CDS             791..955
                     /locus_tag="VF226_10495"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEX7074457.1"
                     /translation="MIGPSLNDNIQEQIGAKLKALFDESVNQAVPDRFVELLQALAEK
                     EDDGAETDGS"
     gene            930..1502
                     /locus_tag="VF226_10500"
     CDS             930..1502
                     /locus_tag="VF226_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7074458.1"
                     /translation="MAPKQTEASFRDGLLAEIPSLRAFARSITGNPDRADDLVQETLV
                     KAWANRSRYTQGTNLRAWLFTILRNTFYSEFRKLTREISDTEGRFAATLAQQASQLAH
                     LDLEEFKRAFAQLPEDQREALFLIGASGFSYEEAAAICGCAVGTMKSRTNRARQKLAT
                     ILEVRSPDDFGPDAIDTAAISSPQKRVARG"
     gene            complement(1567..2100)
                     /locus_tag="VF226_10505"
     CDS             complement(1567..2100)
                     /locus_tag="VF226_10505"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7074459.1"
                     /translation="MARSFVDRWVSRLRRSAREEIQETARSALHYVVLYAITAVAGLM
                     ALVFLTLAVFWSLTLELSPIAAALIITIFYAVVAALVLLWASWNGSAATVSGPHLQEE
                     GAEPENAGDGGTPDVTDMMGVNLDSVARTLASAGFQTESLVVSASSEVLKQLTPLQFV
                     ALVFVGSFLFGRRLRRR"
     gene            2362..2928
                     /locus_tag="VF226_10510"
     CDS             2362..2928
                     /locus_tag="VF226_10510"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX7074460.1"
                     /translation="MKNIVALTAALSMLGATAAVSQTTNQMQQPQTPQVTPQQQTSPP
                     VGVPPSPQAQTPQSKTTPMQQPSGQEAFLSQQQPDQMLATDLIGKSILGSNNARIGDI
                     NDLLISQNGEIVAALVGVGGFLGIGEKVVAIPYDSLNRSPQSDQITVSYSREDLEQAP
                     QFVTAERARSGTGGTGTTGGGSAGGTQR"
     gene            3049..3534
                     /locus_tag="VF226_10515"
     CDS             3049..3534
                     /locus_tag="VF226_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7074461.1"
                     /translation="MRSMEDLFLHSLQDIYTAEKRILQNLPTMAKHVGTSELKKALEQ
                     HKNETQEHVRRLEEVFKLIGQKPKGVECKAIDGIIGEAQEMMQEIEDKEVGDAAILAD
                     AQAVEHYEITRYGTLTAWAKQLGHEDAAKLLHQTLEEEKKTDELLTKMAKGRVNKAAA
                     A"
     gene            complement(3622..4722)
                     /locus_tag="VF226_10520"
     CDS             complement(3622..4722)
                     /locus_tag="VF226_10520"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7074462.1"
                     /translation="MPRIDSTVTTLVIAVALAAAVGYGVNEWTEARKREQQTSSLTAV
                     AQPVPAKSDWVTSAPGRVEPRSGEIRLTAQAPGRVVRILAQAGDQVKAGDVLVLLDEE
                     EARARLAAAEAQEAASRRARDDESVGKQAQDRRNAEDALANAERSLHAARVDLDRLLV
                     ARHSNDASQEEIDRARSNLTAAEERVERERQELGRIAAASDMPLPTRLEAALAQARAE
                     VRLAEIAFDRTRIRAPIDGTVLDVNVKEGEMLAPSPEAFAMTIGDVSGLRVKAEIEER
                     DISKIRVGQRVVVRSSAFEGRDFEGRVSSVAAYVGPPRLGSRGPRKPTDIDILEVMID
                     LDGRPPLMPGMRVDVFFRADVTSEAKRLAGAN"
     gene            complement(4715..5467)
                     /locus_tag="VF226_10525"
     CDS             complement(4715..5467)
                     /locus_tag="VF226_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074463.1"
                     /translation="MSHPVVLEARNIVKELGEGAARVRALKGVSLSLRAGELTLLMGP
                     SGSGKTTLLSILGCILTPTSGSVRIKDETIDGLTPEQLADLRRRHIGFIFQSYNLFPT
                     LTAFENVRLALDVRGMGYAETVAKAESALKAVGLAHKLRSYPRNMSGGEQQRVAVARA
                     LAGAPSVILADEPTAALDSENGHAVMALLAQIARDRNHAVLAVTHDPRTHAYADRIVR
                     IEDGRIVGEDRRPEANKNVKKYDITRKRKANA"
     gene            complement(5464..6594)
                     /locus_tag="VF226_10530"
     CDS             complement(5464..6594)
                     /locus_tag="VF226_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074464.1"
                     /translation="MIVTLAIRNLFHDRVRLIVTLVGILFSIVLVAVQLGLYLGARKM
                     IIDMIEYSNGELWITAYGAKSFEEGGILLTPRERHAALAAPGVAAVVPLVVSFAEWRK
                     PGGGSTNTVIVGSDAEDNGLKAWNIVEGSLSSIEAPDGVAVDRSYLGDLGVKGIGDVA
                     QVGDTRVRIRAITEGIRSFTMAPYIFTTLNRARTLLGVPSENSTYFLVQVVPGTDIEH
                     LRKDLTERLPNVEVLTKAEFRDRSLDQWLFSTGAGVALIGGAILGLLVGTVIVAQTLY
                     SSTKDHLNEFATLRALGSSSGYIHKVILAQAGLSAVIGYVLGMIIALVVVVLSTHTAL
                     PIIMTPGLAVSLFLLTLFMCAISAVSAIMKVTKIDPAMVFSR"
     gene            6934..7482
                     /locus_tag="VF226_10535"
     CDS             6934..7482
                     /locus_tag="VF226_10535"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX7074465.1"
                     /translation="MIQYRMRAAIPRRAAGIRRFAVSAAAFCSLLSGAAFEAGAQTAP
                     PVNSPNASPTPVGIWFDDTGQGAIEIAPCGEMLCGRIVWLKEPTDENGRPLTDALNPD
                     PRNRQRPICGLQIIGDLKPQPDGTWDEGWIYDPKEGKSYDVEIRLRAPDRLQIKGYLG
                     VKFLSESFIWQRAPAELPRCTA"
     gene            complement(7540..8424)
                     /locus_tag="VF226_10540"
     CDS             complement(7540..8424)
                     /locus_tag="VF226_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7074466.1"
                     /translation="MSTQDRLQESEGSLLLPGGRVGVLLIHGLGGTPVELRFIAQALN
                     RAGHTVYCPLLVGHGGSEELLNTTGWTDWYNSVEEAHDLLKERCDVVLAGGLSAGVPL
                     ALLLAARRPEQVHGCLLYSPTFWPNGWAIPWYFNLFKLVRHKWFANLLHFRERAPYGI
                     KDERIRSFVLDSLQSEGRQLDDIFGRRGGTVLEFRWLAKAAKDVLGEVKQPTLIFHPR
                     FDDQSNIKNTLMLQKQLGGLNEVIVLEDTYHMVTLDRQRMLVAEKTIAFVDWLAKRRQ
                     GAEDVERIKQNVAAAGSE"
     gene            complement(8563..9984)
                     /locus_tag="VF226_10545"
     CDS             complement(8563..9984)
                     /locus_tag="VF226_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7074467.1"
                     /translation="MRWAASSEPLHDRQSDLSEAELLRLEAEYCSHGDTVHYTDPPKI
                     FDRCEGSFLYDSEGREYLDLQMWYSAVNFGYRNPRLNNAVKRQLDTLPQVASQYLHRE
                     KIELAAMIARDAEKKFGEKGRVHFNVGGSQAIEDSLKLVRNAKNGKSLMFAFEGGYHG
                     RTLGASAITSSYRYRRRYGHFGERAYFLPFPYHFRGPKGMSKEEYGSYCVAQFARLFE
                     SEYNGVWDPKVGEAEFAAFFVEPLQGTGGYIVPPPNFFKELKKVLDEHGILLVVDEIQ
                     MGFYRTGKLWSIEHFGVKPDVLVFGKAITNGLNPLAGIWAREELINPTVFPPGSTHST
                     FNSNPMGTGVALEVMKMLAETDYEAMVMEKGAYLLEGLKDLQRRHKAIGDVDGLGLAL
                     RMEICEPHDSYTPSKAIVDRMVDEGLKGDIDIGGRRYGCVLDIGGYYKNVVTLAPSLH
                     ITKEEMDLALKLLDALLIRATRD"
     gene            complement(9999..10700)
                     /locus_tag="VF226_10550"
     CDS             complement(9999..10700)
                     /locus_tag="VF226_10550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEX7074468.1"
                     /translation="MSSTRMKFHVFVDFDGTIAPLDTTDLLLQRFADPGWMEIEEQWK
                     AGLIGSRECMVRQVDLIRATPEEFDRFIDEIEIDPGFVHFVRLCSRLGHAVTVVSDGL
                     DRTVGTVLKRAGVNVPFRANRLTWAGGRRWRLAFPHARSDCLTLAGNCKCRFAEAKRA
                     NGRIVVGDGRSDFCIAGRADLVLAKGLLVAHCREQQLPHIEFRDFTEATELLVQWIAA
                     HSEDPAAHPAHIGDE"
     gene            11007..11417
                     /locus_tag="VF226_10555"
     CDS             11007..11417
                     /locus_tag="VF226_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7074469.1"
                     /translation="MANIDTKAAEAEGKQKGERAAIVRNIAEVPWLEFPNHFGGALSK
                     PLVTPDTAGSKHLDYRISCYQPMAYVATHTHTLQEQIYHVLDGEGLMQIDGRSHVVRK
                     HDVIYLPPGVEHTIQNSGLTDLVFLVITSPTQDA"
     gene            complement(11502..13016)
                     /locus_tag="VF226_10560"
     CDS             complement(11502..13016)
                     /locus_tag="VF226_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017265123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074470.1"
                     /translation="MFTETWDVVVVGGGGAGLAAAIEARRLGRDVVLIEKNPALGGST
                     AWSIGSVSATATPHQIRKGIKDGPDGHYADMPGFAGPLANRDNDELRRILCDEMPETF
                     RWLLSHGVRFFGPMPEPPHKQPRMHNVLPNARAYIYHLERAARRAGVAIRTRTRAINL
                     IEESGSVTGVECETSVGTTRFMARGGVILSAGDFTSSPDLKRTYMGEREAAVAGVNPT
                     ATGDGQKLAMAVGGWIVNGDLALGPEIRFVPPARQNLVGRMPPWTWLAEFMAWSLENM
                     PNAILRPFVMSFLTTALAPSPALFQHGAILVNRDGRRFTDELAEPAYDIPGQPDSVAY
                     ILVDGRIAKLFSGWPHYISTAPGIAYAYMPDYRRNRPDVYHEAPSLELLADKMGASRE
                     ALAGAVSEHNAHLGAAAGDAGNRLPLIEPPFTALGPVRAVFVHNEGGLAVDSSHRVLN
                     GSGVPIPGLFAAGSTGQGGLLLKGHGHHLAWAFVSGRRAGRIAALSVSSPPSVA"
     gene            complement(13054..14550)
                     /gene="tcuA"
                     /locus_tag="VF226_10565"
     CDS             complement(13054..14550)
                     /gene="tcuA"
                     /locus_tag="VF226_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEX7074471.1"
                     /translation="MLPEQCDVVVVGGGNAALCAALSAAEQGVSVLVLERAPEAESGG
                     NSRFTAGAIRCVYDGVEDLKVLMPDLSEEEIANTDFGSYTEEKFFDDMGRVTEYRTDP
                     ELTEVLVTRSRETMRWMREKGVRFQPIYGRQAFRVDGKFKFWGGLTVEAWGGGPGLVQ
                     ALTDAARRNGIEIAYLARALSLVHGDSGVTGIRVRHDGRTQTIRTKAVVLAAGGFQAN
                     AEWRTRYLGPGWDLAKVRGTRFNTGDGIRMALEAGAMPTGNWSGCHAVGWDRNAPEFG
                     DLAVGDNFQKHSYPFGIMINANGERFVDEGADFRNYTYAKYGRVILAQPGQFAWQIFD
                     RKVLHLLRDEYRIKQVTKVSANTLEELVAKLDDVDAEKALETIKAYNAAVMTDVPFNP
                     NVKDGRGTRGLAVKKSNWANTIDEPPFEAYAVTCGITFTFGGVKIDTSAQVIDTEGVP
                     IPGLYAAGEMVGGIFYFNYPGGTGLMNGAVFGRIAGASAGRAAREING"
     gene            complement(14566..15426)
                     /locus_tag="VF226_10570"
     CDS             complement(14566..15426)
                     /locus_tag="VF226_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7074472.1"
                     /translation="MKGIKLMTTHAADFGINSYSYTLDHTARQFIDKLADRGYREFEL
                     MIYPGHLWPRDLSAAERSDLRRHLELRGLDVVTLNMPNIDINIAGASDEMRAYSLDLL
                     QQIVELAGDLGVPGVIIGPGKSNPLFPMARENLLGHFFAALDRLAPLAEKVGTQLYLE
                     NMPFAFLPGADELLAALDQYGRSDIAVIYDVANGWFIKEDIAEALRKCARSGRLKLVH
                     YSDTGQTVYRHDPVGEGTVPFAIVPPVLAEIGHTRRPMLEIISRQPDASIDDSVRRLA
                     EMGYGNGRAC"
     gene            complement(15485..16363)
                     /locus_tag="VF226_10575"
     CDS             complement(15485..16363)
                     /locus_tag="VF226_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074473.1"
                     /translation="MSFENVVVTGGAGRLGTYVVDRLRNKCSVTVLDVKPLSGPQADG
                     LRQIQSSITDYAAMRAAFEGQDAVIHLAAIPNPRSAPADVTFNTNVQGAWAVLQAAED
                     AGVKRVVVASSDSVFGLSYNPPDWPPKYLPVDEDHPVRPTEFYSLSKQVTEVIAQSYA
                     HRRKLEVLAIRPVHIVFPPEYPELEARGSDVQNYHFWAYVSPEDVAQGFDLALRSSYK
                     GYEVFLISAADGLNARPTLEMARERWATLPEVRRPEIYEKNPFASVIDTTRARDRLGY
                     EPTSDWRKMIASLQGR"
     gene            16628..18073
                     /locus_tag="VF226_10580"
     CDS             16628..18073
                     /locus_tag="VF226_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7074474.1"
                     /translation="MKRDSALQRATIEIPMLIGGEWRKADEMHDVVDPYRNEIVARAP
                     RSSLADLDAALQSAVAAKPVIAAMPGYERAALLRRVKALLLERADEVAEIMARETGKA
                     IKDAKAEVIRSQDTIELSAEEAIRIAGEQVPLDGSAMGAGKIAMLLRFPVGVVAGITP
                     FNAPFNLACHKIAPALAAGNAIVLKAPPQAPTVVHKLAEMFVDAGTPPGALNVLYGDI
                     VGPALVRDPRVDFITFTGSSRVGAEIKAASGLRRVALELGGDGPVIVAADASIEESAA
                     VCARNSMRLAGQSCISVQNVFVHESLHDAFLERLVEHVKRLKVGDPLDLQTDVGTLID
                     EAAAIRVEAWVKEAVAQGARLLTGGRRNGAQVEPTVLADVKPDMRIACDEVFGPVVGV
                     RRFSDIGEVFQRISASRFGLQCGIFTKSLDLAMRAVREIRTGGVILNGSSTWRTDQLA
                     YGGVKDSGIGREGPKYAIRDMTEERLVVFNL"
     gene            18118..18990
                     /locus_tag="VF226_10585"
     CDS             18118..18990
                     /locus_tag="VF226_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7074475.1"
                     /translation="MARARKELDAIGGGISRPAKLGGDVVKDRGGVQSIERAFAILEE
                     VARHREGIRLGDLSRRVGLHNSTTFHLVKTMVQLGYLRQIPETKYYRIGRPLFTLAAS
                     ALDETEMVSLSTPILEDLSRVTEESSHFAVRSGDKIVVLARTAGTGAFQLTERAGVVR
                     PAHCTALGKVLLASLPPDQFEAFLKRCPLQALTPKTITDPELLRREVEEARANGIAYD
                     DGEFNPEVRCVAVPVRNFSGQVIGAIGISGPVWRLSLAALQDKARLVRERAARLSAEF
                     GYDATGLPKRGARD"
     gene            19154..20119
                     /locus_tag="VF226_10590"
     CDS             19154..20119
                     /locus_tag="VF226_10590"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074476.1"
                     /translation="MAVLITGSMGHAGFELVRQSVQRGMAVVAQYRDTFREADAKALG
                     GTVRWVRCDLTDDADLKSLLDGADIDGCIHTAAIPNELYCRPAPLNAVRTNTGAVASL
                     LEAARRRNWRRFIYVSTGSVFQNADTSKPVFEDAQQAVTNVYSTTKYCGELLTSMYRS
                     QFEISAATVRISWVYGPPLVPKERENPRGPIPYFLKCALMGKPVREPSGGDFAASFTH
                     VSDVAGGLLAAYEAPSLGYDAYHLGSGVNYTTRDVVRAIKSAVPDAEIEVGAGTAPWT
                     DHTRMRGPLAGDRLLNDTGWRPRLSLEDGIRSFAEWMRAHPEVLQ"
     gene            20116..21090
                     /locus_tag="VF226_10595"
     CDS             20116..21090
                     /locus_tag="VF226_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074477.1"
                     /translation="MSLTGSHPQLVKRSRALQLLAELPGAAYLLFLMSVLLSFATPEF
                     FSVANLTNVALQVAVLAIVSFGMTLVILTEGIDLSLGPVLGLCGVVMGLLVVAGWPLA
                     IAVGAALLIGAAFGTMNGILVAYVEMPPFIVTLGAFGIAMSFAMVLTEGNSVTGLPEF
                     VRLFNEGVFLGIPVPIWATAVCFAVTYILLYHTKFGRYTFAIGGNRQALILSGSPARF
                     YQVLVYAYAGALAAVGSFIMTARMNAAHPTIGVGLEFDAIAAVILGGTSFEKGRGGIT
                     GTVIGALAVGVLRNGLNLMGVSTEWQVAVVGIVIILAVSLDSLRGLNQ"
     gene            21087..22097
                     /locus_tag="VF226_10600"
     CDS             21087..22097
                     /locus_tag="VF226_10600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074478.1"
                     /translation="MTVQVVGQRTGMVKPELLGLSPDRLFVVVSRLVALLLIVVTMSI
                     LSPYFLTWSNLINVVRHAAPLFLLSAGLTLVVLTAGIDLSVGAVLGLSACLAASLITG
                     GHPALGILAALGAGLACGLVNGTLVAYASVPPFIATYGMLWIANGLAYIFMQGEVIYG
                     LPSEFRVIGAGFIGPIPVPVLIMIATLIALHVLAHKTPIGRGIYAIGGNPVAARLSGM
                     PVQRRLVFVYAMSGLMASFAALVVIARTNAADVSLGEEQLLPAIAAVCLGGTSLFGGV
                     GGVAGTAVGAIILALILNGMNLLGIGTFWQSGALGFIILVSVFTDQIVGGRIGRMSGV
                     TQ"
     gene            22166..23131
                     /locus_tag="VF226_10605"
     CDS             22166..23131
                     /locus_tag="VF226_10605"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7074479.1"
                     /translation="MVASAAAALGTFPFRSAIAQGGKPTIAFFVKNVTNPFWKACRIG
                     AEKAAKELDVNLEVVAPTKPDNIEEQTRLVEDWIVKKPDAFVFVPVDYKALVPSIQKA
                     NEAGIPIINYNNKMTGIEKVTFIGSDDALFEYEICKYLFNSMGGKGKVVHIDGVPAAI
                     TAQMRKQGFERAVKEFPGIEVLASQPGQYRRLPAAQVFENLMQRFPEIDAVVSANDDM
                     AVGIVEALHAAGRGGQTKVVGIDGIPDAIQAIAEGKMFATVDFSGHDQAYLGVQAAVR
                     HLKGEKVPQEILLPVFIIDKSNVDKVARTPEERPVPSWDKVVGKA"
     gene            23222..24025
                     /locus_tag="VF226_10610"
     CDS             23222..24025
                     /locus_tag="VF226_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7074480.1"
                     /translation="MTEMTLPLVSMRGICKRYGGVQALNDVSLDIYAGEVHALVGDNA
                     AGKSTLIKILAGAVARDSGTIHFDGREVDIHTPRDAKALGIETVYQDLALADNLDVAS
                     NIFLGRELTLSGPLKPFLDIRRMEEDARKLLGRLKINIPSIRQKVRSMSGGQRQSIAI
                     ARSVYFNARIVILDEPTAALGVEETRKVYDLVREMRSQGLAVLMISHNLNHVFENSDR
                     ITVLKTGRYVGSRRVVETTRDEILRMIVSGEADPPATEAREQAPRVAVS"
     gene            24051..25106
                     /locus_tag="VF226_10615"
     CDS             24051..25106
                     /locus_tag="VF226_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074481.1"
                     /translation="MPEKTALVIGATGAVAKRLIELLSADDNWAVVGLCRKPPLADRR
                     VRWISADLGEPAQCRDALAAHPDITHVFYCARAKHGEGGVESVDENVAMLRNVLDAVE
                     HTAQNLRHVHLVEGGKWYGLHLGRYPTPAHEDDPRHMPPNFYYDQEDLLRTRQQGKAW
                     TWSASRPNVICDFAPERPRNLVSIIGAYAAICAELGLRFDFPGKPGNFTALTELTDAS
                     LLARGMVHIATSPACQNKPFNITNGDLIRWEREWPRIARLFGLEPGTIRTLPLARWME
                     DKEPVWRRIVARHGLVPRRLDEVAVWGFADFVFGQDYDVVSNLNRLRSSGFHDFVDTS
                     EMLIGQLQQYREARILP"
     gene            25142..26047
                     /locus_tag="VF226_10620"
     CDS             25142..26047
                     /locus_tag="VF226_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074482.1"
                     /translation="MQFEKVLVTGAGGLLGGYVVNELRGRARVSGLDITPSKGEIPFT
                     RGSIEDPAAVSKACQGQDAVIHIAARPNIWSGTGTEIMGTNVVGTWNVLQAAEEAGVK
                     RIVLTSSDSVFGYTVMSGAMIPPDYLPVDSDHPLRATDPYALSKVLCEQMGRSFSLRG
                     KMEVLVIRPVYVLYPEFECEVKARAANPTGYNGPEAGGRQPAGGGPMWHYVDPRDLAR
                     AFRLALEVKNPRFGAYVISARSTLAPEPTVERLARIMGRQIPVRKPEVYSANPFAPLY
                     DLAPARDDLGFAAEHDLRSLLYPAT"
     gene            26065..26385
                     /locus_tag="VF226_10625"
     CDS             26065..26385
                     /locus_tag="VF226_10625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX7074483.1"
                     /translation="MPVLHTAHLRTHPHAVELYKARLLQHARNSLEREPGGCLRFDVH
                     QDRSDPTLFFLIEVYRDEAALEAHRTSPHFLEYREDTKDWVTDRQWWYWTPLELPPAG
                     RTGR"
     gene            26382..27185
                     /locus_tag="VF226_10630"
     CDS             26382..27185
                     /locus_tag="VF226_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074484.1"
                     /translation="MTAYSNHSFLPVDLTGQKALVTAGGSGIGLVIAEAFLARGARVW
                     ICDIDEAALQQARERLPGLHASVVDVSKPDQVAGLFADIRATFGGLDVLINNAGIAGP
                     TAKIEDVSPGALAETLDVNVGSHFYCAAEAVPMMKAAGSGTIINLSSIAGRLGFSMRS
                     PYAASKWAVIGFTKSLAVELGEHNIRVNAILPGHVRTERFKRVAAAKAQALGITPEEM
                     RQRMLEPVSLKRNVEVEDIANMALYLASPFGRNITGQAISVCGDVQMMR"
     gene            27251..28153
                     /gene="rlmJ"
                     /locus_tag="VF226_10635"
     CDS             27251..28153
                     /gene="rlmJ"
                     /locus_tag="VF226_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEX7074485.1"
                     /translation="MNYRHSYHAGNFADVLKHATLALVIEHLKGKPGAFRVIDTHAGA
                     GLYELDKGPAEKTGEWRQGIGRLVGPHADPLPDDVQKLLAPYLAAVAAENAPGELDLY
                     PGSPRLARTLIRPQDRLIANELHPEDGQLLKELFSRDSQAKVLHLDGWTALKALLPPK
                     ERRGVILVDPPFEEPGELERLKRGLREAAERFPTGIYLLWYPIKDPKPIASFHRSVAA
                     LGLPKLLVAELMIRSSHVVTRLNGSGLVILNPPFQLDETLNQILPFLADRLGQDSGAG
                     FRITWLVGEDSENAQTHNLKLRSR"
     gene            28381..28596
                     /locus_tag="VF226_10640"
     CDS             28381..28596
                     /locus_tag="VF226_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7074486.1"
                     /translation="MRQTGTVKFFNQTKGFGFITPDQGGKDVFVHVTAVERSGIGSLD
                     EGMRVSFETEPDKRGKGPKAINLQTSA"
     gene            28761..29078
                     /locus_tag="VF226_10645"
     CDS             28761..29078
                     /locus_tag="VF226_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074487.1"
                     /translation="MLKLTGALVAGVGAAVLLLGAANPADARTCINKAGQGTNTTEEG
                     AKFQAYEAILQATDWGMWANWMATSQKIGVAPGYAVSRVRFKCQKGTGLGSTCVGQAT
                     LCK"
     gene            29246..29509
                     /locus_tag="VF226_10650"
     CDS             29246..29509
                     /locus_tag="VF226_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074488.1"
                     /translation="MKTLSAIALGAALSAGLAFAPAAQAAKCVMAGGSATGLTQDIAK
                     QLATMALNQSISNYGGKASGKIALKCDANLVMSSCTAKQRVCK"
BASE COUNT         5687 a         9480 c         9186 g         5518 t
ORIGIN      
        1 gcgcgcctcg tccctcgtac gtgccacggc gatgacacga tggccaagct cctcgagaat
       61 ctgcgtcaga tccattacga tcagcggctc gtcctcgatg atcaaggcgt ccgttgcgac
      121 ctgacgggcg atctcctcgc cggccttctc gagcagctcg tgtacctctt ccacgctggc
      181 gtccaggatc tcggccgtct ctgggagggt gaaaccctcg acgtgccgca gcaggaaagc
      241 ctgccgcgag cgcggggtca ttgcggccac gttgcgatcg aaccccgaag ccaaggcctt
      301 gggcgatggt tgccagccgc tggcagcaac cgatgttatg atctgtatga atgtcttgta
      361 gagggcgacg cgagcgtcgc tggacttcgc cagcgacgct gggtctacta caagcgtttc
      421 caagagctgc tgtacgtacg catccccgcc ggcctgcgaa ccggtaagcg cacgcgcata
      481 acgccgcaga tgagggatgt gcgagccgac aacgcttgcc atcgacatga gatacgcccc
      541 tattttacta cccagacgct tagcggtcgc cttatggcat cctacactgg gaacgcgtca
      601 ggcaccagtt agttgcaacg gttaggggtt ttttgtcgtc ccatggaact gctaccctct
      661 ctgccgcgtt tggtctacgg gcgccggact gcgcacctcc gcacggggtt catcgatgaa
      721 taatgcagcc aagcgaaagg ctttgcccgc aagcaaagca cgggatcccc gcgcgcagga
      781 ggacgaacgg gtgattggac ctagtctgaa cgacaacatc caagagcaga ttggcgcaaa
      841 gctcaaggcc ttgttcgacg agtccgtcaa ccaggctgtc cccgacaggt tcgtggagct
      901 tctgcaagcg ctcgcagaaa aggaagacga tggcgccgaa acagacggaa gctagcttcc
      961 gcgacggcct cctggccgaa ataccgagcc tgagggcctt tgcacgttcc attacaggta
     1021 atcctgaccg cgctgatgat ctcgtccagg aaactctggt caaagcgtgg gccaatcgct
     1081 ccaggtatac ccagggcacg aacttgcgtg cctggctgtt cacgatcctg cgcaacacct
     1141 tctactcgga atttcgcaag ctgacccgcg agatctccga cacggagggg cggtttgcag
     1201 caacgcttgc ccagcaggcc tcgcaactcg cacacctcga cctcgaggag ttcaagcggg
     1261 ccttcgccca gctgcccgaa gaccagcgcg aagccctgtt cctcatcggc gcgtccgggt
     1321 tctcctacga ggaagcggcc gccatatgcg gctgcgcggt cggaacgatg aagagccgca
     1381 cgaaccgcgc acggcagaag ctggcgacca tcctcgaggt gcgctcgccc gacgacttcg
     1441 ggcccgatgc catcgatacg gcggcgatct cgtcaccaca aaagcgcgtc gcacggggct
     1501 gatctcccgc gcctttctcc ccgccttgct tggatgttga cgttcacccc tcgcgtgcga
     1561 gggcgttcat cgccgccgca gccgtcggcc gaacaggaag ctacctacga aaacgagcgc
     1621 tacgaactgt agcggtgtca gttgcttgag gacttcgctg gacgctgaaa ccacgaggct
     1681 ttccgtctgg aagccggcgc tggccagcgt cctggccacg ctgtctaggt tcacccccat
     1741 catgtcggtc acgtccggcg tgccgccatc gcccgcattt tccggctccg cgccctcttc
     1801 ctgcaagtgc ggtccactca cggttgccgc gctcccgttc caggaagccc agagcagcac
     1861 gagcgcagcc acgaccgcat agaaaatcgt tatgatgagc gcagcggcga tgggggaaag
     1921 ctcgagggtc agcgaccaga ataccgcgag cgtgaggaaa acgagcgcca tcaggccggc
     1981 gacggcagtg atcgcataca ggacgacgta atggagggcg ctacgagcag tttcctgaat
     2041 ttcctcgcga gcgctcctcc tcagccggct gacccacctg tctacaaatg atcttgccac
     2101 ggattcgtcc ttccgggacg gatgtccctc cgaggccctc cgctgcctgg ctggctgcag
     2161 ccaagcgctc acgcgtttgc catcgtcgtt gcgaaacgct gccgcatgcc gagcgttccc
     2221 ttcaggtagg cccgggccga acgcttcgca gagaccttgc aaaccctgca ccagccagcc
     2281 ggcaggcaat cctttggccg gaacccatcc ctgcgtcggt cgttcaaaga ggcaaatgcc
     2341 aatggcaaat ggagagtcaa cgtgaagaat atcgtagctc tgaccgcggc cctcagcatg
     2401 ctgggtgcaa ccgcggccgt atctcagacg accaatcaga tgcagcaacc gcagacacct
     2461 caggtgaccc cgcagcagca gacatcacct cctgtcggag tgccgccatc gccgcaggcc
     2521 cagactccac agtccaagac cactccgatg cagcaacctt cgggtcagga ggctttcctg
     2581 agccagcaac agcctgatca aatgctggcg acggatctga tcgggaagag cattctcggc
     2641 agcaacaatg cgcgtatcgg cgacatcaac gacttgctga tctcgcagaa cggcgagatc
     2701 gtcgccgctc tcgtcggcgt cggcgggttc ctcggcatcg gcgagaaggt cgtggccatc
     2761 ccctacgact cgctcaatcg ttcgccgcag tcagaccaga tcaccgtctc gtactcacgc
     2821 gaggatctcg agcaggcgcc tcagttcgtg acggcggaac gcgcaaggag cggcaccggc
     2881 ggcacgggta cgaccggcgg cggcagtgcc ggcggcaccc agcgctgaca tctttcgaat
     2941 agggcagtcc gctgctgcag cggactgccc tctcctattg cgcggaacca ttgcgggact
     3001 tcagccgtat aggccaacaa ctgtccctga ggagattgca acgtgccgat gagatcgatg
     3061 gaagacctgt tcctgcacag cctgcaggac atctacacgg cggagaagcg gattctgcaa
     3121 aacctgccga ctatggcgaa gcacgtgggc acgagcgagc tcaagaaagc gctcgagcaa
     3181 cacaagaacg agacgcagga acacgtcagg cggctcgagg aagtcttcaa gctgatcggc
     3241 cagaagccga aaggcgttga atgcaaggcg atcgatggca tcatcggcga ggcccaggag
     3301 atgatgcagg agatcgagga caaggaggtc ggcgacgcgg cgatcctcgc cgatgcgcag
     3361 gccgtggagc attacgagat cacgcgctac ggcacgctga cggcctgggc caagcaactt
     3421 ggacacgagg atgcggcaaa gctcctccac cagacgctgg aggaagaaaa gaagacggac
     3481 gaattgctca cgaaaatggc caagggccgc gtgaacaagg cagcggcggc ctgaaacccg
     3541 cacccccctc gaaccaaaaa gggctggcgc ccacaaaagc gccagccctt tttcgtccgg
     3601 tagccgcttc gcagcaggcg atcaattcgc gcccgcaagc cttttggcct cggaggtcac
     3661 gtcagcccgg aagaagacgt cgacacgcat gcctggcatg agcggcggcc ggccgtccag
     3721 atctatcatc acttccagga tatcgatgtc tgtcggcttg cgcggtcccc ggctgccgag
     3781 ccgaggcggg ccgacgtagg ccgccacgct gctcactctg ccttcgaaat cacgcccctc
     3841 gaaggcgctt gaccgcacga caacacgctg gccgacgcgg attttgctga tgtcgcgctc
     3901 ctcgatctcc gctttgacgc gcagccccga gacatcgccg atcgtcatcg caaaagcctc
     3961 aggtgacggc gccagcatct caccctcctt cacgttcacg tcgagaacgg tgccgtcgat
     4021 cggtgcacgg atgcgggtcc ggtcgaaagc gatctcagcg agccgtacct cggcgcgcgc
     4081 ctgcgccaag gctgcttcca gcctcgtcgg cagcggcata tccgaagctg ctgctatgcg
     4141 gcccagttcc tggcgctcac gctcgacccg ctcttcggcc gcggtcagat tggatcgcgc
     4201 acgatcgatt tcctcctggc tcgcatcatt gctgtgacgc gcgaccagca gccgatccag
     4261 atcgacccgg gcagcatgca acgagcgttc cgcattggcc agcgcatcct cggcgttgcg
     4321 tcgatcctga gcctgctttc cgacgctttc atcatcgcgt gcgcggcggc tcgcagcctc
     4381 ctgggcctcc gcggcagcga ggcgggcgcg tgcttcctct tcatcgagga gaacgagcac
     4441 atcgccggct ttgacctgat cgccggcctg ggccaggatc ctgaccacgc ggccaggcgc
     4501 ttgcgcggtg agccggatct cgccgctcct tggctccacg cgacccgggg ccgatgtgac
     4561 ccagtccgac ttcgcgggca caggctgtgc gacggcggtc aacgagctcg tctgctgctc
     4621 acgcttgcgc gcctcggtcc attcgttcac gccgtatccg accgcggcgg cgagcgcaac
     4681 cgcgatcacc agcgtcgtga cagtcgagtc gatcctaggc attggccttt cgctttctcg
     4741 tgatgtcgta ttttttcaca ttcttgtttg cctcggggcg gcggtcctcg ccgacgatcc
     4801 ggccatcctc gatgcggacg atccggtcgg cataggcgtg cgtgcgtgga tcgtgcgtca
     4861 ccgccagaac cgcgtgattg cggtccctgg caatctgcgc cagcaatgcc atcaccgcgt
     4921 gcccgttctc gctgtcgagt gcagctgtcg gctcgtcggc caggatcacg gacggagcac
     4981 cggcaagggc ccgggcaact gcgacgcgct gctgctcgcc gccgctcatg ttgcgcggat
     5041 agctgcgcag cttgtgggcg agcccgacgg ccttcagtgc actttcggct ttcgccacgg
     5101 tctcggcgta gcccatgccc cgcacgtcga gcgccaggcg gacattctcg aaggccgtca
     5161 acgtcgggaa gagattgtag gactggaaaa tgaagccgat gtgacggcgc cgcagatcgg
     5221 cgagctgctc gggcgtcagc ccgtcgatgg tctcgtcctt gatacgtacc gaacccgagg
     5281 tcggcgtcag gatgcagccg aggatcgaca gcagggtcgt cttgccgctg ccggaagggc
     5341 ccatcagcag cgtcagctcg ccggccctca gggacaggct gacgcccttg agcgcccgca
     5401 cgcgggccgc gccctcgccc agctccttga cgatgttgcg cgcctcgaga acgacggggt
     5461 gactcatctg ctgaacacca tggctggatc gatcttcgtg accttcatga tcgccgatac
     5521 ggccgagatc gcgcacatga agagcgtcag caggaacagc gagaccgcca gtcccggcgt
     5581 catgatgatg ggcagtgccg tgtgcgtgct gagcaccacg acgacgagcg cgatgatcat
     5641 acccagcacg tagccgatga ccgcgctgag acccgcctgc gccaggatca ccttgtgaat
     5701 gtacccggat gacgaaccga gagcacgcaa ggtcgcgaac tcgttgagat ggtccttcgt
     5761 gctcgaatag agcgtctgcg ccacgatgac ggtgccgacc agcaggccga ggatggcacc
     5821 gccgatcagc gccacgccgg cacccgtcga gaagagccac tgatcgaggc tgcggtcgcg
     5881 gaactcggcc ttggtcagca cctcgacatt gggcaggcgc tcggtcaagt ccttgcgcag
     5941 atgctcgata tccgtgccag gcacgacctg cacgaggaag tacgtcgaat tctcactcgg
     6001 cacgccgagc aacgtccgtg ctcgattaag cgtcgtgaag atgtacggcg ccatcgtgaa
     6061 cgatcggatt ccctcggtga tcgctcgtat gcgcacacgg gtatcgccga cctgggcgac
     6121 gtcaccgatg cccttcacgc cgaggtcgcc gagatagctg cgatccaccg ccacgccgtc
     6181 cggcgcttct atcgaggaaa gcgaaccttc gacgatattc cacgccttga ggccattatc
     6241 ctccgcgtcc gatccgacga tcacggtatt cgtgctgccc ccgcccggct tgcgccattc
     6301 ggcaaacgac acgacgagcg ggacgaccgc agcgacgccg ggggcggcaa gtgccgcatg
     6361 gcgctcacgc ggggtgagca ggataccgcc ctcctcgaag cttttggcgc cgtaggccgt
     6421 gatccaaagc tcaccgttgc tgtattcgat catgtcgatg atcattttgc gtgcgccgag
     6481 gtaaagccca agctgcacgg caacgagcac gatcgaaaac aaaattccga cgagcgtgac
     6541 gatcagacga acgcgatcgt gaaacagatt gcgaatggcc agcgtcacta tcatcgggtt
     6601 tccaaggtca gatcagttac acgcgatcgg gatgactatc cgggcacatg cgaatgtgaa
     6661 gtaccgcagg ttacacctgg ccgcatttcg agcaattcgt tgggcatcga tccagtgcga
     6721 gccatcccgt gagagcccgc tccttataga aggtctgcca tcatggcgcc actccccaga
     6781 gcgagaggag ttaagtatcg gcctgagaag tcatgcctcc acctcggcga atgagcgtac
     6841 gagtttggca cttccaaggc atatcatccc gaccatggag ctgattgcac cttgcaaatg
     6901 agtgcgtgag gatcgttcat gaagtctgat cacatgatcc aatatcgaat gcgcgctgca
     6961 attccgcggc gagctgccgg catcaggcgt tttgctgtca gtgccgcggc tttttgctcg
     7021 cttctgtcgg gtgcggcatt tgaagccggg gcacaaaccg ctccgccggt caactctccc
     7081 aacgcatcac cgacgcccgt cggtatctgg ttcgatgata ccggacaggg cgcgatcgaa
     7141 atcgctccct gcggcgaaat gctttgcggc cgcatcgtct ggctgaaaga gcccaccgat
     7201 gagaatggtc ggccattgac ggatgctctc aatcccgatc cccgcaaccg gcaaaggccg
     7261 atttgcggcc tccagatcat cggcgacctc aaaccgcagc cggacggcac ctgggacgag
     7321 ggctggatct acgaccccaa ggagggcaag tcctacgacg tcgagatcag actacgcgcc
     7381 cccgaccgac tccagatcaa aggttatctc ggcgtcaaat tcctcagcga atcctttatc
     7441 tggcaacggg cgccggctga gctgccccgc tgcaccgcgt aagcgacaat agaaattgag
     7501 atagagccgc cgggagcgta gaaccgcggc tcatcgcgac tattcgctgc cggctgcggc
     7561 cacgttctgc ttgatgcgct cgacgtcctc ggccccctgg cggcgcttcg cgagccaatc
     7621 gacgaacgcg atggtctttt cggcgaccag catccgctgc cggtccagcg taaccatgtg
     7681 ataggtgtcc tccagcacga tcacctcgtt gagaccgccg agctgctttt ggagcatcaa
     7741 cgtattcttg atgttgctct gatcatcgaa gcgcggatgg aaaatcaggg tcggctgctt
     7801 gacctcgccg agcacgtcct tggcagcttt ggccagccat cggaattcca gcacggtacc
     7861 accccggcgg ccgaagatgt cgtcgagttg ccggccttca ctctgcagtg aatccaaaac
     7921 gaaactgcgg atgcgctcat ccttgatgcc atagggcgcg cgttcccgga aatgaagaag
     7981 attggcgaac catttgtgcc ggaccagctt gaatagattg aaataccagg gaatggccca
     8041 gccgttcggc cagaatgtcg gcgaatagag aaggcagccg tgaacctgct ccggcctgcg
     8101 cgccgcaagc agcagtgcca ggggaacacc ggccgataga ccgccggcga gtacgacgtc
     8161 acagcgctcc ttgagcaggt cgtgcgcctc ctcgacggag ttgtaccaat ccgtccagcc
     8221 cgtcgtgttg agcagctcct cggatccccc gtgtccgaca agaagcggac aatagacggt
     8281 atggccggct cggttgagag cctgtgcgat gaagcgcaac tctaccgggg tgccgccgag
     8341 gccatggatc agcagaacgc cgacacgccc tccgggcaac aacagcgacc cctcgctctc
     8401 ctgcagccga tcctgcgtac tcattgacgt cgcttcttaa ctctcgttga actcgatgac
     8461 tgcactcacg ccacgttgcc cgaaccatct aatggtccgg gccactcgtt ggttcgggcc
     8521 tagagcgttg atgcgaatgc tttgccagct cccattcggc aatcagtcgc gggttgcgcg
     8581 gataagcagc gcgtccagca gcttgagagc cagatccatt tcctccttcg tgatgtgcag
     8641 gctgggggcc agcgtcacca cgttcttgta atagccgccg atatcgagca cgcagccgta
     8701 gcgacgcccg ccgatatcga tgtcaccctt caagccctcg tcgaccatcc ggtcgacaat
     8761 ggccttggac ggcgtgtagc tatcgtgcgg ctcgcagatc tccatgcgca gagccaagcc
     8821 cagcccatcg acatcgccga tggccttgtg ccgccgctgc aggtctttca ggccctccag
     8881 cagataggcg cccttttcca tcaccatggc ctcgtaatcc gtctcggcca gcatcttcat
     8941 gacctcgagc gcgacgccgg tgcccatggg attggaattg aacgtcgaat gcgtcgagcc
     9001 gggcggaaat acggtcggat tgatcagctc ctcgcgcgcc cagatgcccg ccagcggatt
     9061 gaggccattc gtgatcgcct tgccgaacac gagcacgtcc ggcttgacgc cgaaatgctc
     9121 gatcgaccag agcttgccgg tccgatagaa gcccatctgg atctcgtcca cgaccagcag
     9181 gatgccgtgc tcgtcgagaa ccttcttgag ctccttgaag aagttcggcg gcggcacgat
     9241 gtagccgccc gtgccctgca gcggctcgac gaagaaggct gcgaactcgg cctcgcccac
     9301 cttggggtcc cagacgccgt tgtattcact ctcgaacagc cgcgcgaact gagcgacgca
     9361 atagctgccg tattcctcct tgctcatgcc tttggggccg cggaaatggt acggaaacgg
     9421 caggaaatag gcgcgctcgc cgaaatggcc gtagcgtcgg cgatagcggt aggaagacgt
     9481 gatggcagac gcgccgagcg tgcggccatg atagccgccc tcgaaggcga acatcaggct
     9541 cttgccattc ttggcgttgc ggacgagctt gaggctgtcc tcgatcgcct ggcttccacc
     9601 cacgttgaag tgaacacgcc ccttctcgcc gaacttcttc tcggcatcgc gggcaatcat
     9661 cgcggccagc tcaatcttct cgcgatgcag gtactggctc gcgacctgcg gcaaggtgtc
     9721 gagctgccgt ttgacggcat tgttcagccg gggattacga tagccgaagt tgacggccga
     9781 gtaccacatc tgcaggtcga gatactcgcg gccttcgctg tcatagagga acgagccttc
     9841 gcagcgatcg aagatcttgg gcggatccgt atagtggacc gtgtcgccat gggagcagta
     9901 ttcggcttcg agcctcagga gctccgcctc gctcaggtca ctctgccggt cgtgcagcgg
     9961 ctcagagctg gccgcccagc gcatcggttt cgacatcgct actcgtctcc aatatgagcc
    10021 ggatgggctg ccggatcctc agagtgggcc gcgatccact ggaccagaag ctcggttgcc
    10081 tcggtgaaat cgcggaattc gatatgtgga agctgctgct cccggcaatg cgcgacgagg
    10141 agacctttcg cgaggaccag gtcagcacgt cctgctatgc aaaagtccga tctcccatcg
    10201 ccaaccacga tacgtccatt ggcgcgtttg gcctccgcga accggcattt gcagttaccc
    10261 gcgagcgtca gacagtcgct gcgggcatgg ggaaatgcca gccgccagcg ccttcccccg
    10321 gcccacgtca gccgattggc gcggaagggc acgttgacgc cggcacgctt cagcacggtg
    10381 ccgacggtcc tgtccagtcc gtcggagacg accgtgaccg cgtgtcccag cctgctgcaa
    10441 aggcgaacga agtggacgaa ccccggatcg atctcgattt catcgatgaa gcgatcgaat
    10501 tcttcgggag tggccctgat gagatcgacc tggcggacca tacactcgcg cgagccgatg
    10561 aggccggctt tccattgctc ctcgatctcc atccagcccg gatcagcgaa tcgctggagg
    10621 aggaggtcgg tcgtatcgag aggcgcaatc gtcccatcga aatcgacgaa cacatgaaac
    10681 ttcatacgcg tactcgacac gtgcaaacct gccaatacat ccgacgagtg aatatgccga
    10741 cagtatcccc ggggcgatag tccctttagc gaccggcaat cgccatgcca atggttttcg
    10801 ttaagggtgc gactatcgat tgctccgata ggtgacgctc aagctcaagt ccgattgcac
    10861 ataatcgcgt ggaaggacga ttgctagccc tgaattgtgg tgcagcacat cggtagctgc
    10921 agatcagcca atctcagcag gagctcttgg tctgcaatta tggtaaacca aggctctatc
    10981 atcatagcaa gaggaacgct cagctaatgg cgaatattga cacgaaagcg gcagaggccg
    11041 agggaaaaca aaaaggtgaa cgcgcggcga tcgttcgcaa catcgccgag gtgccgtggc
    11101 tcgagttccc caatcacttc ggcggcgcac tctcgaagcc gctcgtgacg cccgatactg
    11161 ccggctcgaa gcacctggac tatcggatct cctgctacca gccgatggcc tacgttgcca
    11221 cccacacgca cacgttgcag gaacagatct atcacgtcct cgacggcgaa ggtctgatgc
    11281 agatcgacgg caggagccac gtcgtccgca aacatgacgt catttatctg ccgcccggcg
    11341 tcgagcacac gatccagaac tctggcctga cggacctcgt cttcctggtt atcacgtcac
    11401 ccacccagga cgcgtaatct ccagccctgc aaccataggt aggtggtgcg agcctgcgca
    11461 cgcatcacct gctctccttc gggcagatcg ccaagatcgg tctaggccac cgatggcggc
    11521 gatgaaaccg agagtgcggc aattcgccca gcccggcggc cggagacgaa ggcccaagcc
    11581 agatgatggc catggccctt cagcagcagg ccgccctgtc cggttgaacc ggcggcgaac
    11641 agcccgggaa tcgggacacc gctgccattg agtacacgat gcgagctatc gacggccaag
    11701 ccgccctcgt tgtgtacgaa gacggccctg acgggaccca gcgccgtgaa cggaggctcg
    11761 atcaagggaa gtcgattgcc ggcatcgccg gctgcagccc cgaggtgggc attgtgctcg
    11821 gatacggcac ctgccagcgc ctcgcgcgac gcgcccatct tgtcggccag gagttcgagg
    11881 ctcggcgcct cgtggtaaac atcgggccga ttgcggcgat agtcgggcat ataggcatag
    11941 gcgatgccgg gggccgtcga gatgtagtgt ggccagccgg aaaacagttt cgcgatgcgc
    12001 ccgtcgacca gaatgtaggc gacgctatcc ggctggccag ggatgtcgta tgcgggctcc
    12061 gcaagctcgt ccgtgaaccg tctgccgtcc ctgttcacca ggatcgcacc gtgctggaac
    12121 agggccggtg acggcgccaa ggccgtcgtg aggaagctca tcacgaacgg ccgcaggatg
    12181 gcattcggca tgttttccag cgaccatgcc atgaactccg cgagccaggt ccacggcggc
    12241 atgcgcccga ccagattctg acgcgcaggc ggcacgaagc gaatttcggg gcccagcgcc
    12301 agatcgccat tgacgatcca gccgccgacg gccatggcca acttctgacc gtcacccgtg
    12361 gcggtcgggt tgacgcccgc caccgccgcc tcgcgctcgc ccatgtaggt gcgcttgagg
    12421 tccggcgagc tcgtgaaatc acccgcgctc aaaatcacgc cgccgcgtgc catgaagcgg
    12481 gtcgtaccga ccgaggtctc acactccact ccggtgacgg acccactctc ctcgatcaga
    12541 ttgatggctc gcgtccgcgt gcggatggca acgcccgcgc gccgggcggc acgctccagg
    12601 tggtagatgt aggcacgcgc gttcggcagc acgttgtgca tgcgcggctg cttgtgcggg
    12661 ggctcgggca ttggtccgaa gaagcgaacg ccgtgcgaca gaagccagcg gaacgtctcc
    12721 ggcatctcgt cgcaaaggat gcgccgcagc tcgtcgttgt cgcgattggc aagcggtccg
    12781 gcgaaaccgg gcatgtccgc gtaatgccca tccggtccgt ccttgatgcc tttgcggatc
    12841 tgatgcgggg tcgccgtcgc ggaaacggag ccgatcgacc acgccgtaga gccgccgagg
    12901 gcgggattct tttcgatcag cacgacgtcg cggccgaggc gccgcgcctc gatcgccgcg
    12961 gccagccctg cccctcctcc gccgacaaca acgacgtccc aagtctccgt gaacacgctc
    13021 cgccccacat tgcgatcgca ctagcgcaaa gagctagccg ttgatctccc gtgctgcccg
    13081 gccggcgctg gcgccggcga tccggccgaa cacggcgccg ttcataagcc ccgtcccgcc
    13141 aggataattg aaatagaaga tgccgccgac catttcgccc gcagcataga ggccaggtat
    13201 cggcacgcct tccgtgtcga tgacctgcgc cgaggtgtcg atcttcacac ctccgaacgt
    13261 gaaggtgatg ccacacgtga ccgcatacgc ctcgaacggc ggctcatcga tggtgttcgc
    13321 ccagttcgat ttcttcacgg ccagcccgcg cgtgccgcgg ccgtccttca cattcggatt
    13381 gaagggcaca tcggtcatga cggccgcgtt gtaggccttg atcgtctcca gcgccttctc
    13441 ggcatcgaca tcgtcgagct tggcgaccag ctcctcgagc gtattggccg aaaccttcgt
    13501 gacctgcttg atccggtatt cgtcgcggag caggtgcagc accttcctgt cgaagatctg
    13561 ccaagcgaac tgtccgggct gcgccaggat cacacggcca tacttggcat aggtgtagtt
    13621 gcggaagtcc gcgccttcgt ccacgaagcg ctcgccgttg gcgttgatca tgatgccgaa
    13681 cgggtagctg tgcttctgga agttgtctcc tacggcgaga tctccgaact cgggcgcatt
    13741 gcggtcccag ccgacggcat ggcagcccga ccagttgccg gtcggcatgg cgccggcttc
    13801 caatgccatg cggatgccgt cgcccgtgtt gaaccgcgta ccacgcactt tcgcgaggtc
    13861 ccagccagga ccaagatagc gcgtgcgcca ttcggcattc gcctggaatc cacctgcggc
    13921 gagcacgaca gccttcgtcc tgatggtctg ggttctgccg tcatggcgaa cgcggatgcc
    13981 ggtgacgccg ctatcgccat ggacgagcga caacgcccgg gccaggtaag cgatctcgat
    14041 cccgttcctg cgcgccgcgt ccgtcagcgc ctgcaccaat ccggggccgc cgccccaggc
    14101 ctccaccgtc aatccgcccc agaacttgaa cttgccgtcg accctgaacg cctgccggcc
    14161 gtagatgggc tggaaacgaa cgcctttttc gcgcatccag cgcatcgtct cgcggctgcg
    14221 cgttacgagc acctccgtca gctcggggtc ggtccggtac tcggtcacac ggcccatgtc
    14281 atcgaagaac ttctcctcag tgtacgagcc gaaatccgtg ttggcgatct cctcttcgct
    14341 gagatccggc atgaggacct taaggtcctc gacgccatca tagacgcagc ggatcgcgcc
    14401 cgccgtaaag cggctgttgc cgccggactc ggcctccggc gcgcgctcca gaaccagcac
    14461 cgaaacaccc tgctcggcag ccgagagagc ggcgcaaagg gcggcgtttc ccccaccgac
    14521 gacgacgacg tcacattgct caggcagcat ccaactccct ccagatcaac aggcccgccc
    14581 attgccatag cccatctcgg ccaagcgtcg cacgctatca tcaatgcttg cgtcgggctg
    14641 gcgggagatg atctccagca tggggcgccg ggtatgcccg atttctgcga gaacaggcgg
    14701 cacgattgca aacgggaccg tgccctcgcc aaccgggtcg tgccgataga ctgtctgccc
    14761 cgtatcggaa tagtggacca gcttcaaccg gccggatcgc gcacatttgc gcagcgcctc
    14821 cgcgatgtcc tctttgatga accagccgtt ggccacatcg tagatcaccg caatgtcgga
    14881 tcgtccatac tggtcgaggg cggcgagaag ctcgtcggcg ccgggcagga atgcgaaggg
    14941 catgttctcc agatagagct gcgtgccaac tttctccgcg agaggtgcca gccggtcgag
    15001 ggcggcgaag aagtggccaa gcagattttc gcgcgccatc ggaaagagcg gattgctctt
    15061 gcccggcccg atgatcacgc cgggcacgcc gagatcgcct gccagctcga cgatctgttg
    15121 aagcaggtcg agcgaatagg cgcgcatctc atcggaggcg ccggcaatgt tgatatcgat
    15181 gttgggcatg ttgagcgtaa cgacgtcgag ccctctcagc tcgagatggc ggcgcagatc
    15241 gctgcgctct gcggcactca ggtcgcgcgg ccataaatgg ccgggataga tcatcagctc
    15301 gaactcgcga tagccgcgat cggcgagctt gtcgatgaac tgccgagcgg tgtgatcgag
    15361 cgtataggaa taggagttga tcccgaagtc ggcagcgtgg gtcgtcatga gcttgatgcc
    15421 tttcattgag cttagctctt acggaggcgg ctcatccgcg aggctaagag agatccgcga
    15481 tctgtcaccg gccctgcagg ctcgcgatca ttttgcgcca gtcgctggtc ggctcatagc
    15541 cgaggcggtc tcgagcccgc gtggtgtcga tcacggatgc gaacgggttc ttttcgtaga
    15601 tctccggacg gcggacctcg ggcagagtgg cccagcgttc tcgcgccatt tcgagcgtcg
    15661 ggcgcgcgtt gagcccgtcg gcggccgaga tcaggaacac ttcgtagccc ttgtatgagg
    15721 acctcagcgc cagatcgaag ccctgcgcca cgtcctcggg cgagacgtag gcccagaaat
    15781 gatagttctg gacgtcgctg ccgcgcgcct cgagctccgg gtattccggc gggaagacga
    15841 tatgcaccgg gcgaatggcg agcacctcga gcttgcgccg gtgcgcatag ctctgagcga
    15901 tgacctccgt cacctgcttg ctcagggaat agaactccgt cgggcggaca ggatggtcct
    15961 catcgacggg caggtacttt ggcggccaat caggcggatt gtaagacaga ccgaaaaccg
    16021 agtcgctgga ggcgacgacg acccgcttca cgccggcatc ctcggcggcc tgcagcacag
    16081 cccaggcacc ctgcacattc gtgttgaagg tcacgtcggc aggggcgctg cgcgggttgg
    16141 gaatggccgc cagatggatc accgcatcct gaccttcaaa ggccgcgcgc atcgcggcgt
    16201 aatcggtgat gctggactgg atctggcgca agccatcggc ttgcgggcca ctgagcggct
    16261 tcacgtcaag cacggtcacg gagcacttgt tcctaagccg gtcgacgaca taggtgccga
    16321 gcctgccagc tccgcccgtg acgactacgt tctcgaagct caagggcgtc tcccattctc
    16381 acgcactgac gttgcagtgc attgtcgaga ttattttcac attgtgcgaa gtcaacccac
    16441 catctgagac aatgatctta gcggctgacg ccgtgcctcg tcacgcgctt cgaagaggct
    16501 atgagcggtt gacgtcaaaa cgacgacgcc aagatgtgaa ttgacgtttc acgatatgag
    16561 atcatcatta ttggaaaccg ccgaaaggcg cgagcgggcg atattcgact aggaagggcg
    16621 aaacttcatg aaaagggaca gcgcgttgca gagggccacc atcgagattc cgatgctcat
    16681 cggcggtgaa tggcgcaaag ccgacgaaat gcacgatgtc gtcgatcctt atcgcaacga
    16741 gatcgtcgcc cgcgcccctc gctcctcgct tgccgatctc gatgcggcgc tgcaatcggc
    16801 tgttgcggcg aagccggtca tagccgcgat gcccggctat gagcgggcgg cgctgctccg
    16861 gcgcgtcaag gcactcctgc tcgagcgcgc ggacgaggtc gctgagatca tggcgcgcga
    16921 gaccggcaag gccatcaagg acgccaaggc cgaggtcatc cgatcccagg acacgatcga
    16981 actgtccgcg gaggaagcga tccgcattgc cggcgagcag gtgccgctcg atggctcggc
    17041 gatgggggca ggcaagatcg ccatgctgct gcgctttccc gtcggcgtgg tcgcggggat
    17101 caccccgttc aacgcgccgt tcaacctcgc ctgccacaag attgccccag ctctggccgc
    17161 tggcaatgcc atcgtgctca aggcgccgcc gcaggcacca accgtcgttc acaagctcgc
    17221 cgagatgttc gtcgacgcgg gcacgccacc gggcgccctc aacgtgcttt atggcgacat
    17281 cgtcggaccg gcgctggtgc gggatccccg cgtcgacttc atcaccttca ctggctcgag
    17341 ccgcgtcggc gccgagatca aggcggcatc aggcctgcgc cgagttgcac tggagctcgg
    17401 tggcgatggt ccggtcatcg tggccgctga tgcctccatc gaggaaagcg ccgcggtctg
    17461 cgcgcgcaat tccatgcggc tcgccgggca aagctgcatt tcggtgcaga acgtcttcgt
    17521 gcacgagagc cttcatgacg cctttctcga gcgcctggtc gagcacgtga agcggctcaa
    17581 ggtcggcgat ccgctcgacc tgcagaccga tgtaggtacg ctcatcgacg aggccgcagc
    17641 aattcgcgtc gaggcctggg tgaaggaggc cgtcgcgcag ggcgcgcgcc tgctcacggg
    17701 cggccgccgg aatggggcgc aggtcgagcc caccgtgctc gccgacgtga agcccgacat
    17761 gcgcatcgcc tgcgacgagg tgttcggccc ggttgtcggg gtgcgccgtt tcagtgatat
    17821 cggcgaggtt tttcagcgta tcagcgccag ccgcttcggc ctgcagtgcg ggatcttcac
    17881 gaagtctctt gatctggcga tgcgggccgt tcgcgagatc cggaccgggg gcgtcattct
    17941 caacggatcc tcgacttggc gaacggatca gctcgcgtat ggtggcgtca aggacagcgg
    18001 catcggacgc gaaggtccaa agtacgctat cagggacatg accgaggaac gactcgtcgt
    18061 gttcaaccta taggcgcttc tgcaggggcc ggccgaatga agaagcgagg agatgatatg
    18121 gccagggcga gaaaggagct cgacgccatc gggggtggga tttcgcggcc cgcaaagtta
    18181 ggcggggacg ttgtcaagga ccgtggcggc gtgcagtcga tcgagcgcgc attcgccatt
    18241 ctcgaggaag tggccaggca tcgcgaaggc atcaggctcg gcgacctcag ccggcgagtc
    18301 ggacttcaca acagcaccac gtttcacctc gtgaagacga tggtccagct cggctatctg
    18361 cgccagatcc cggagacgaa gtattatcgc atcggccgtc ctctctttac gctcgcggcc
    18421 agcgcgctcg acgaaaccga gatggtgtcg ctgtcgactc ccatccttga ggatctgtcg
    18481 cgggtgacag aggaaagcag ccatttcgcg gtacgctcgg gcgacaagat cgtcgtgctg
    18541 gcgcgcacgg ccggaactgg cgccttccag ctcaccgagc gcgccggggt ggtccgcccc
    18601 gctcactgca cggccctcgg caaagtgctt cttgcctcgc tgcctcccga ccagttcgaa
    18661 gccttcctga agcgctgtcc gctgcaggcg ttgaccccca aaacgatcac cgatcccgag
    18721 cttctcaggc gcgaggtcga ggaggcccgc gcgaacggca tcgcctacga cgacggcgag
    18781 ttcaatccgg aggtccgctg cgttgccgtc cccgtgcgca atttctcagg ccaggttatt
    18841 ggcgccattg gaatttccgg cccggtctgg cggctttcgc tggccgcatt gcaggacaaa
    18901 gcaaggctgg tacgcgagcg cgcggcgagg ctttcagcag aattcggcta cgatgccacc
    18961 ggtttaccca agcgcggcgc tcgggattga actgttaagt ttatattgac agccgtttcc
    19021 gtctcggcga ttttctaaac aagaaacgtg ctcccacatg atgaaataaa atcgggagcg
    19081 gaggaaactc accgcgccgt cgtgggaagg gcaaagcact tgccatgccg cgcattcagc
    19141 acgagacgaa ttcatggcgg tgctgattac agggtccatg gggcatgcgg gcttcgagct
    19201 cgtccgccag tctgtccaac gcggcatggc cgtggtcgcg caataccgcg acacattccg
    19261 cgaggctgat gcgaaggccc tgggcggcac cgtacgctgg gtgcgctgcg acctgaccga
    19321 cgacgcggat ttaaaaagtc ttttggacgg tgccgatatc gacggctgca tccacacggc
    19381 ggcgatcccc aacgagctct actgccgtcc cgcccccctc aatgcggtgc gcacgaacac
    19441 cggcgcagtc gcgtccctgc tggaagcggc gcggagacgg aactggcggc gtttcatcta
    19501 tgtgagcacc ggttcagtct ttcaaaacgc cgacacatcc aaaccggtct tcgaggacgc
    19561 tcagcaggcg gtcacgaacg tctacagcac cacgaagtat tgcggcgaat tgctgaccag
    19621 catgtatcgt tcgcagttcg agatatccgc ggcgaccgtc cggatctctt gggtttacgg
    19681 accgccgctg gttccaaaag agcgcgaaaa ccctcgtggt cccatcccgt acttcctcaa
    19741 atgcgcgctg atgggaaaac cggttcgcga gccgagcggt ggtgatttcg cggcgagctt
    19801 cactcacgtt tcggatgtgg ccgggggatt gctggcagcc tacgaggcgc cctcgctcgg
    19861 ctatgacgcc tatcacctcg gctcgggcgt gaactacacg acgcgcgacg tcgtgcgtgc
    19921 catcaagtct gccgttccgg acgctgagat cgaggttggc gctggtactg cgccctggac
    19981 ggatcacacc cgcatgcgcg gcccgctcgc aggggaccgt ctgctgaacg acacgggatg
    20041 gcgcccgcgc ctgagcctcg aggacggcat ccgatccttc gccgaatgga tgcgggcaca
    20101 ccctgaggtt ctgcaatgag cctgacggga tcacaccccc agctcgtcaa gagatcgcgt
    20161 gcgctgcaat tgctcgcgga gctgccgggc gctgcatacc tcctgttcct gatgtcggtg
    20221 ctgctgagtt tcgcgacgcc cgagttcttc tccgtcgcga atctcacgaa cgtcgcgctg
    20281 caggtcgccg tcctggcgat cgtatctttc ggaatgacgc tggtgatcct gaccgaaggc
    20341 atcgacctca gcctcggacc ggtgctgggg ttgtgcggcg tggtcatggg attgctcgtc
    20401 gtcgcaggat ggcctttggc tatcgctgtc ggagcggcgc tcctgatcgg cgcggctttc
    20461 ggaacgatga acggaatcct tgtcgcctac gtcgagatgc cgccgttcat cgtgacgctc
    20521 ggcgccttcg gaattgcaat gtcgttcgcg atggtgctga ccgagggcaa ctccgtgacg
    20581 ggattgcccg agttcgtacg gcttttcaac gagggtgtct ttctcggcat tccggtgccg
    20641 atctgggcga cggctgtttg tttcgccgtc acgtacatcc tgctctatca caccaagttc
    20701 ggccgctaca cgttcgcaat aggcggcaat cggcaggccc tgatcctctc cggcagcccc
    20761 gcccgctttt atcaggtgct cgtctacgct tacgcggggg cgctcgcggc ggtgggctca
    20821 ttcatcatga cggcgcgcat gaacgcggcg cacccgacca tcggcgtcgg gctcgagttc
    20881 gatgccattg ccgccgtgat tctgggcggc acctcgttcg agaaggggcg tggcggaatt
    20941 accggcaccg tcatcggagc gctcgccgtg ggcgttctgc gcaacgggct caacctgatg
    21001 ggtgtctcga cggaatggca ggtcgccgtg gtgggcattg tcatcattct cgccgtcagc
    21061 ctcgactcac ttcgggggct caatcaatga cggtgcaggt cgtggggcag aggacgggga
    21121 tggtgaagcc cgagttgctg gggttgtcgc cggaccgcct gttcgtcgtt gtttcgaggc
    21181 tcgtcgccct tttgctgatc gtggtgacga tgtccattct cagcccctat ttcctgacct
    21241 ggtcgaatct gatcaacgtc gttcggcatg ccgcgccgct tttcctgctg tccgccggcc
    21301 tcacgctggt ggtgctgacc gccggtatcg acctgtctgt cggcgcggtg ctcggattgt
    21361 ctgcctgcct cgcggcttcg ctcattaccg gcggccatcc cgcgctcggc atccttgccg
    21421 cactgggcgc cgggctcgcc tgcggtctcg tcaacggaac gctggtcgcg tacgcgtccg
    21481 tgccgccgtt cattgcgacc tacggtatgc tgtggatcgc caacgggttg gcctacattt
    21541 tcatgcaggg cgaggtgatc tacgggctcc cgtctgagtt tcgcgtgatc ggggccggct
    21601 ttattggacc tattccggtt cccgttctga tcatgatcgc cacgctgatt gcattgcatg
    21661 tcctagcgca caagacgcca atcggccgtg gcatctatgc cattggcgga aacccggtcg
    21721 cggcgcggct gtcgggaatg cccgtgcagc gccggctggt cttcgtctat gccatgagcg
    21781 gactgatggc gagctttgca gcgctcgtcg tgatcgcgcg tacgaatgca gccgatgtga
    21841 gtctcggcga ggagcagctc ctgccggcaa ttgcagctgt ttgcctcgga ggcacctccc
    21901 ttttcggcgg ggtcggtgga gttgccggaa ctgcggtcgg cgcaatcatt ctggccctca
    21961 ttctcaacgg tatgaacctg ctcggcatcg ggacgttctg gcagtcaggt gcgctcggct
    22021 tcatcattct ggtctcggta ttcaccgacc agatcgtggg aggacgaatc ggacggatga
    22081 gcggcgtcac ccaataaacc aataacggct tggcaatgga ggaaacgcaa tgaaaataag
    22141 caggcgtcaa ttcacagcag gaacaatggt tgcgagcgct gctgcagcac tcggcacttt
    22201 tcccttccga tccgcgatcg cgcagggcgg aaaacccacg atcgcatttt ttgtaaagaa
    22261 cgtcaccaac cccttctgga aagcctgccg catcggcgcg gaaaaggctg caaaagagct
    22321 cgacgtcaat ctggaggtcg tcgcgcccac caagccggat aacattgaag aacagacccg
    22381 ccttgtcgag gactggatcg tcaagaagcc tgatgctttc gttttcgtgc ccgtcgacta
    22441 caaggccctt gttccgagca ttcagaaggc gaacgaggca ggcattccga tcatcaatta
    22501 caacaacaag atgaccggca tcgagaaggt cacgttcatc ggctcggacg acgcgctgtt
    22561 cgaatacgag atctgtaagt atcttttcaa ttcgatgggc ggcaaaggca aggtcgtgca
    22621 catcgatgga gtgccggccg ccatcacggc tcaaatgcgc aagcagggtt tcgagcgcgc
    22681 ggtcaaggag ttccccggca tcgaggtgct ggcctcacaa cccggccaat atcggagact
    22741 tccggccgca caggttttcg aaaacctgat gcagcgtttc ccggagatcg acgccgtagt
    22801 gtcggcgaat gacgatatgg cggtcggcat cgtcgaggcg ctgcacgccg caggccgcgg
    22861 cgggcagacg aaagtcgtgg gaattgacgg tatcccggat gcgatccagg cgattgccga
    22921 gggcaagatg tttgccaccg tcgacttctc gggccacgac caggcttatc tcggcgttca
    22981 agccgctgtg cgccacctca agggcgagaa ggtgccacag gagattctgc tgccggtgtt
    23041 catcatcgac aagagcaacg tcgacaaggt tgcccgcact cctgaggagc ggcccgtccc
    23101 ctcctgggac aaggtggtcg gcaaagcctg aggacaatat cagcggatgg cgggcatctc
    23161 ggcaccgcca ttcgctaacg cttgcatccg ggcgagatat cgaacccgaa cgggagacgc
    23221 gatgaccgaa atgacactgc cactcgtgtc gatgcgcggc atctgcaagc gctatggcgg
    23281 cgttcaagca ctgaacgacg tcagtctcga catctacgca ggcgaggtgc acgcgctggt
    23341 cggcgacaac gcagccggca aatcgacact catcaagatc ctggcgggtg cggtggctcg
    23401 cgactcagga acgatccatt tcgatgggcg cgaggtggat attcatacac cgcgcgatgc
    23461 gaaggccctc ggcatcgaga ccgtctacca ggacctggcg ctcgccgata atctggacgt
    23521 cgcgtcgaac atctttctag gccgcgagct gaccctcagc gggccactga aacccttcct
    23581 cgacatccgc cgcatggaag aggatgcacg caagctcctc ggccggctca agatcaatat
    23641 tccttcgata cggcagaagg tgcgctcgat gtcgggtgga cagcggcaga gcatcgccat
    23701 cgcccgcagc gtttacttca acgccaggat cgtcatcctc gatgagccga cggccgcgct
    23761 cggggtcgag gaaacgcgga aagtttacga cctcgtgcgc gagatgcgca gccagggcct
    23821 cgcggtgctg atgatcagcc acaaccttaa tcacgtgttc gagaacagtg atcgcattac
    23881 ggtgctcaag acgggccgat acgtcggctc gcgccgggtc gtggagacga cgcgcgacga
    23941 gatcctgcgg atgatcgtgt caggcgaggc cgacccgccg gcgacggaag cccgggaaca
    24001 agccccacgc gtggcggtga gctgacccgc agccagcccg ggacgcctcc atgccggaaa
    24061 aaaccgcact tgtcatcggt gccacgggcg cagtcgccaa gcggctgatc gagctgctgt
    24121 ctgctgatga caattgggcg gtggtcggac tctgccgcaa gccgccgttg gctgaccgtc
    24181 gcgtccgatg gatctcggcc gatctcggcg agccggccca atgccgggac gccctcgcgg
    24241 cgcatccgga tattacccac gtcttctact gcgcgcgcgc caagcacggc gaaggcggcg
    24301 tcgaaagcgt cgacgagaat gttgccatgc tgcgcaacgt gctcgatgcg gtcgagcaca
    24361 cggcgcagaa ccttcgtcac gtgcatctcg tggaaggcgg caagtggtac ggcctgcatc
    24421 tcgggcgtta tccgaccccc gcgcacgagg acgacccgcg gcacatgccg cccaacttct
    24481 actacgacca ggaggacctg cttcgcacgc ggcagcaagg gaaagcgtgg acatggtccg
    24541 cctcgcgccc caacgtgatc tgtgatttcg cgccggagcg gccgcgaaac ctcgtttcca
    24601 ttatcggtgc ctacgcggcc atctgtgccg agctcgggct tcgcttcgat ttcccgggaa
    24661 agcccggcaa tttcacggcc ctgacggagc tgaccgatgc cagcctgctc gcgcgtggca
    24721 tggtgcacat cgcaacgagc ccggcctgtc agaataagcc tttcaacatc accaatggcg
    24781 acctgatccg ttgggagcgc gagtggcccc gtatcgcccg gctgttcggg ctggagccgg
    24841 gcaccattcg aaccttgccg ctcgcccgct ggatggagga caaggaacct gtgtggcggc
    24901 gcatcgtcgc cagacacggg ctcgtgccgc gccggctcga cgaggtcgcc gtgtggggct
    24961 ttgccgattt cgtgttcggt caggactacg acgtcgtctc gaacctcaac aggctgcgca
    25021 gctccggatt tcacgatttc gtcgacacca gcgaaatgct catcgggcag cttcagcaat
    25081 accgcgaggc gcgcattctg ccctgagtgt ccattggcgc gcggacaagg gattactggc
    25141 gatgcagttc gaaaaggttc tcgtcaccgg cgcgggcggt cttctcggtg ggtacgtcgt
    25201 caacgagctg cggggacgag cccgggtgtc cggtctcgac atcacgccga gcaaggggga
    25261 aattccattc acacggggct cgatcgaaga ccctgccgcc gtgtccaagg cgtgccaggg
    25321 gcaggatgcc gtcatccaca tcgccgcgcg tccgaatatc tggagcggca ccggcaccga
    25381 gatcatgggg accaacgtcg tcggcacatg gaacgtgctg caagctgcgg aggaggccgg
    25441 cgtcaagcgc atcgtcctga cctccagcga ctcggtgttc ggctataccg tgatgtcggg
    25501 cgcgatgatc ccgcccgact atctcccggt cgactccgat cacccattgc gggcgaccga
    25561 tccctatgcg ctgagcaaag tgctctgcga gcagatgggc cgaagctttt cgctgcgcgg
    25621 caaaatggag gtgctggtca tccggccggt gtatgttctt tatcccgagt tcgagtgcga
    25681 ggtgaaagcc cgtgccgcca atccgacagg atacaatgga cctgaagccg gtggccggca
    25741 gcccgccggc ggtggaccaa tgtggcacta cgtcgacccg cgcgacctcg cccgcgcttt
    25801 ccggctcgca ctggaggtga aaaacccgcg cttcggggcg tacgtcattt ccgcccggag
    25861 cacgctcgct ccagagccga ccgtcgagcg actcgcacga atcatgggtc ggcaaattcc
    25921 agtgaggaag cctgaggttt actcggccaa tcctttcgcg ccgctctacg accttgcacc
    25981 tgcgcgcgac gacctcgggt tcgcagccga gcacgacctg cgcagcctgc tttatcccgc
    26041 aacgtgagca atggatccgc atccatgccg gtcctccata ccgcacacct cagaacgcat
    26101 ccgcacgcgg tcgagcttta taaggcccgg ctcctacagc acgcacgcaa cagcctcgag
    26161 cgtgagccgg gtggctgcct caggttcgac gtgcatcagg accggtccga cccgacgttg
    26221 ttcttcctga tcgaggtcta tcgcgacgag gcagcgctcg aggcgcaccg gacctcgccg
    26281 cacttcctcg agtatcgcga agatacgaag gactgggtca ccgatcgtca gtggtggtac
    26341 tggacgccgc tcgagctgcc gccggccggg aggactggcc gatgaccgcg tattccaatc
    26401 attccttcct gcccgtcgac ctcaccgggc agaaggcgct cgtgaccgct ggtggctccg
    26461 gaatcggtct cgtcattgcg gaggcgttcc ttgctcgagg tgcgcgggtc tggatctgcg
    26521 atatcgatga agctgcgctg cagcaggccc gcgaaagact tcccggactt cacgccagcg
    26581 tcgtcgacgt gtcgaaaccg gaccaggtgg ccggcctgtt tgcggatatt cgagcgacgt
    26641 tcggcggcct cgacgtactc attaataatg ccggcattgc aggacctacg gcgaagatcg
    26701 aggacgtcag ccccggtgcg ctcgctgaga cactcgacgt caatgtgggc agccatttct
    26761 attgcgcggc cgaagcagtg cccatgatga aagccgccgg gtcaggcacg atcatcaatc
    26821 tcagctcaat tgcaggtcgg ctcgggtttt ccatgcgcag cccttacgcg gcatccaagt
    26881 gggccgtgat cggctttacc aagagcctcg cagtggagct cggcgagcac aacatccggg
    26941 tgaatgccat tctccccggg catgtacgca cggagcggtt caaacgcgtt gcagccgcca
    27001 aggcgcaggc gcttggcata acgcccgagg aaatgcggca aaggatgctc gaacccgttt
    27061 ccctgaaacg caacgtcgag gtcgaggaca ttgccaatat ggcgctctat ctcgcctctc
    27121 cattcggccg caacatcaca ggccaggcga tcagcgtctg cggcgatgtg cagatgatgc
    27181 gttaggcgat gcgacgagcg gcggaacggc tgcttgactt gccgcagcca aggcgcgcaa
    27241 cggttgcgtc atgaactacc gccattccta tcacgccggc aatttcgcgg acgtgctgaa
    27301 gcacgcgacg ctggcgctcg tcatcgaaca tctgaagggc aagccaggtg cctttcgcgt
    27361 catcgatact catgccggcg ccggcctcta cgagctcgat aaggggccgg cagagaagac
    27421 aggcgaatgg cgacagggta tcggcaggct ggttggtcct catgcagatc cgctgccgga
    27481 tgacgtgcaa aagctgctgg cgccatacct cgccgcggtc gcggccgaga acgcgcccgg
    27541 cgagctcgat ctctatccgg gaagcccgcg ccttgcgcgc acgctcatca ggccgcagga
    27601 ccgtctgatc gccaacgagc tgcatcccga ggatggccag ctcctgaagg agctcttcag
    27661 ccgggacagt caggccaagg tgcttcacct cgacggctgg acggcattga aggcgctgct
    27721 accgcccaag gagcgtcgcg gggtcattct cgtcgatccg ccgttcgagg agcccggcga
    27781 gctggagcgg ctgaaacgag ggctgcgcga ggctgccgag cggttcccga ccggcatcta
    27841 cctattgtgg tatcccatca aggacccgaa gcccattgct tccttccatc ggtccgtcgc
    27901 ggcgctcggg ctgccgaagc tgctcgtcgc agagctgatg atccgctctt cgcatgtcgt
    27961 cacccggctg aacggaagcg gcttggtcat ccttaatccg ccgtttcaac tggatgagac
    28021 cctgaaccaa atcttgccct tcctggccga tcggcttggc caggactctg gggcgggctt
    28081 ccggatcaca tggcttgtcg gcgaagactc tgagaacgca caaacacaca acctgaaatt
    28141 gcgttcacgc tgatttgcct tgcgcggatc agtctcatcg cgcatagttg tcgcagttcg
    28201 ggcggcctat cggacccggc ggattacgct gagcctgctg atgctacttg cgcacctgcg
    28261 tcgcgatctc gatcgcacct cacgattccc cgacaatcgg atcggactgc ctggctacga
    28321 gggctcgtgc ctttggaggc acgggcaaat gccaccacta cggggacaag gagacctcgc
    28381 atgcggcaga ctggaactgt caagttcttc aaccagacca aagggttcgg cttcataacg
    28441 ccggatcagg gcggcaagga cgtgttcgtt cacgtcactg ccgtcgagcg ctcaggcatc
    28501 ggcagcctcg acgagggtat gcgcgtatcg ttcgagaccg agcccgacaa gcgcggaaag
    28561 ggccccaagg ccatcaacct gcagacctct gcctgaagca gctttgcatc caaagctctg
    28621 cgcggcagtg ccactcctct gcaacgctcc ctgcaaaatt caatccgggt aggcaccgat
    28681 ccggattgaa gcaataggaa attaaggcgg actagggcag agtcgctccg tccgcttttg
    28741 tttttcaaag gagtacttcg atgctcaagc tgacgggtgc gcttgtcgcc ggtgtcggcg
    28801 ctgctgtcct cttgctgggt gcggccaatc cggccgatgc caggacctgc atcaacaagg
    28861 caggccaggg cacgaacacc acggaagagg gcgccaagtt ccaggcctac gaggctatcc
    28921 ttcaggcgac cgactggggc atgtgggcca actggatggc taccagccag aagatcggcg
    28981 tagcgcccgg ctatgctgtc agccgtgtcc ggttcaagtg ccagaagggc acgggcctcg
    29041 gctcgacctg cgtcgggcag gcgacactct gcaagtgacc tgaagatgtc ggccgggtga
    29101 cggtccccta ttcaagtcgt tgcccggctg ccacgcaatg ttggcaatca gcgcgaaagc
    29161 tcacgcggca ccgcgcttac agcttaaccg acgaaatcct tcttgagcat actgtggcgt
    29221 tctgattttt gctcaggagg caaacatgaa gaccctgtca gccattgctc tgggtgccgc
    29281 cctcagcgcg ggcttggcat tcgcaccggc ggcccaggca gcaaaatgcg tgatggccgg
    29341 cggctctgcc acagggctga cccaggacat cgccaaacag cttgcgacga tggcgctcaa
    29401 tcagtccatc agcaactatg gcggcaaggc gagcggcaag atcgcattga agtgcgacgc
    29461 caacctggtc atgtcgagct gcaccgccaa gcagcgcgtt tgcaagtaat ccaaccggac
    29521 accatcgatt agccccggat tatttgacgt caggggccat ccttcgatca cgggccgaat
    29581 ctggcgccga ccgtgcggcg ccagtcaggt gcggccgggt tctatctgcc ccgcaataca
    29641 ctcgttgacc tggcggaagc cgtccgttga cgcacacgcg ttgcgtgcag cccttggcca
    29701 ccggcctggc ctcacgaatt tgtgatcagc cttctgaatt tagctcatag gcgcgcaacc
    29761 actgaccgtc ggcggcgttg atagggcctg gcgagtgaac cgctgattca cgtctcgcgt
    29821 gtattgttga tttatgcacc cttgagtaaa ggcaggtctg tgatgaagcg t
//
LOCUS       DASVDU010000168         6580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1552357, whole genome shotgun sequence.
ACCESSION   DASVDU010000168 DASVDU010000000
VERSION     DASVDU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552357"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1458..1682)
                     /locus_tag="VF226_10655"
     CDS             complement(1458..1682)
                     /locus_tag="VF226_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074489.1"
                     /translation="MGIAASLASPGELILQDFADAVPAADKLDRLIAVIEGTRCFRLS
                     YATVSSAADVVCREVGSGHPSDLGVSHVHP"
     gene            complement(1758..2237)
                     /gene="greA"
                     /locus_tag="VF226_10660"
     CDS             complement(1758..2237)
                     /gene="greA"
                     /locus_tag="VF226_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX7074490.1"
                     /translation="MATERVPMTIEGYKQLEAELHRLKSVERPRIIQAIAEARSHGDL
                     SENAEYHAAKEAQGMNEAKVAELEDKLSRAEVIDPSTLSGDTVKFGATVTLVDEDTEE
                     QVKYQIVGEFEANVREGRISIGSPIARALIGKSKGESVEVTTPKGSRSYEIVSVEFK"
     gene            complement(2357..5638)
                     /gene="carB"
                     /locus_tag="VF226_10665"
     CDS             complement(2357..5638)
                     /gene="carB"
                     /locus_tag="VF226_10665"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX7074491.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGTQACKALKAEGYRI
                     ILVNSNPATIMTDPELADATYVEPITPEIVAKIIAKERPDALLPTMGGQTALNTALAL
                     HKQGVLAEYGVEMIGARADAIDKAEDRQLFREAMGKIGLETPRSMLAHSMSEAMAALE
                     YVRLPAIIRPSFTLGGTGGGVAYNREEFVEIIERGLDASPTHQVLVEESVLGWKEYEM
                     EVVRDKADNCIIVCSIENVDPMGVHTGDSITVAPALTLTDKEYQIMRNASIAVLREIG
                     VETGGSNVQFAVNPADGRLVIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LDNDITGGATPAAFEPTIDYIVTKIPRFAFEKFPGADATLTTSMKSVGEVMAIGRTFA
                     ESLQKALRSMETGLTGLDDIEFEGLGQGDDENVIRAALGTPSPDRILKVAQALRLGLD
                     HEQVHASCKIDPWFIERLQEIVDAEARVAAYGLPPTAEQLRDLKAMGFSDARLAKLAG
                     TTEADVRRRRKELGVEPVYKRIDTCAAEFASPTAYMYSTYERGLDGKPECEAQPSDRE
                     KVIILGGGPNRIGQGIEFDYCCCHACFALTKAGYETIMVNCNPETVSTDYDTSDRLYF
                     EPLTAEDVLEIIRTEQANGKLKGVIVQFGGQTPLKLAGALEEAGVPILGTSPDAIDLA
                     EDRDRFKALIDRLGLRQTESGIARSPSEARSVADAIGYPVMIRPSYVLGGRAMEIVHD
                     GADLDRYVARLSATLDRPSELVVSERRPLLIDRYLSDAIEVDVDCIADGKDTFVAGVM
                     EHIEEAGIHSGDSACSLPPHSLDEATIAELERQTRELALALGVVGLMNVQFAIKDGVV
                     YILEVNPRASRTVPFVAKVIGLPIASIAAEVMAGKPLASFGLKPRKLDHIGVKEAVFP
                     FARFVGVDPILGPEMRSTGEVMGLNSDFAMAFGKSQIGAGQALPTSGTAFVSVKDKDK
                     DRVVAPVRDLVEMGFRIIATRGTKRHLEQHGIPCEHINKVLEGRPHIVDAMKNGEVAI
                     VFNTTEGARALADSKDIRRTALLNHIPYYTTLAGAIAVTKAIRALKSDTLKVAPLQGY
                     AGTAA"
     gene            6001..6441
                     /locus_tag="VF226_10670"
     CDS             6001..6441
                     /locus_tag="VF226_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7074492.1"
                     /translation="MSIEDEIRTLQQIPMFRDLEVSKLKLMAFAGQRITCRRGELLFD
                     EGDPPDAVYVILEGEVEVIRTGAAGEVVLARLGKNELIGEIGVLCNKCRNARIIAQSD
                     VQALRIDKITFMQIVQQVPQLAMAIIRELSERLEHTSEKLAARG"
BASE COUNT         1214 a         2067 c         2038 g         1261 t
ORIGIN      
        1 ggataatcaa ggcttcggaa ccattcgccg cggccgcact ctctgctcta gctaccgttc
       61 gtccttacca atttgcagga atgatacgga gggcttaagt ctcacgtgag acctcttaaa
      121 accaaagatt tcgtaaacgc cgtcaactat ctgagcggtc gttgggtggg aatttcgcgg
      181 gtcttgcccc cacgaattaa gctgtttaaa gttctctgca gtaaagaact cgcgaaaatc
      241 gcaaatgtcc tccacgactt tgtcaatact ttcatttgcc cgtctgagtt tcctatcggc
      301 aatgatgcgg tcattctccg gaacaacggc caagtgctga agaacttcct ccggatcgga
      361 ggatatatac aaggcttgaa gtaatggccg cgcctccctc aatactttcg tgggctcgaa
      421 cctgacctta aaaatgacac gagttccgcg aactgtccca accggaacga agaccgattg
      481 gcgccgagcc ccgtcccctg tctcgctctg gccgtcatct ggtactacca gaaaatttcc
      541 gtgctggcgg gacatttgtc ttagcacgcg caagagtggg ccagcccttt gccggagttt
      601 tcggcgcagc ctttctgcct cagcagctgc cttttcaagg gtgtcaagca aagcgagcct
      661 tccaggcaca aggggcaaat gcaatagtag acgatcgtct acctgctgca gttcaattct
      721 tcgcgcatat tctctgtttg cggcccgagt gacattgctg tcggcacaat catgcccaat
      781 cagatatagg cacttaaggt tcacagcata aactaagcgc ccatcgagaa attttcctgt
      841 tccagggctg caaatagcgc aaggtattcc ttcgagattc gggcgctgac gccgttcaac
      901 cgcgaacggc gctataatta cgtagggttc attcttgctt agcggttcgt ggcaaattcc
      961 gttgaaagtt tcaggtaaac ccgtctttcg aacgtgctca agaaactctg atttgagatg
     1021 ataaggcggt tcgctgaact ggcgtggaac accgttggca ttctgtgtag cgacattccg
     1081 tctcacatcg cccccgagat atagccgaat cacaattcgg cattgtttgc gcgagcagca
     1141 ctcaaaccac cccttaattc taggttatcc accagatttc tttcatctca gcagaaccac
     1201 taccaacccc accttccgta tcctccccaa taccgcccgc agatcctccg caacacctcc
     1261 cgctccacct ccggaaaagc cgctgcgaac agatcgacga gctcccgcag cgtccgcggc
     1321 tgctccagcg cgcgccacac cgcagccgcc atcggatcga ggtggtggtt cgtgtcagtg
     1381 gtagggacgg cggcgaaaaa gggagtcgtc gacctgacga gccgcgatgt cgggggcgcg
     1441 gacgtaaagg agcggcatca tgggtggacg tgagatacgc cgaggtcgga cggatgcccc
     1501 gatccgactt cgcggcaaac aacatcggcc gcggacgaca ctgtggcgta gctcaggcgg
     1561 aaacagcgcg tgccctcgat caccgcgatc agccggtcga gcttatccgc cgccggcacg
     1621 gcatcggcga agtcctgcag gatcagctcg ccggggctgg cgagactagc cgcaataccc
     1681 aagcccgcac accgcgcttg ccgcacgcgt ttggtcgacc ggtcggccct tgtggctcag
     1741 gccgacccgg caggtcgtca cttgaactcc acgctcacga tctcgtagga ccggctgccc
     1801 ttgggggtcg tgacttcgac ggattcgcct ttcgacttgc cgatgagagc acgggcaatg
     1861 ggggaaccga tggagatccg cccctcacgg acattggcct cgaactcgcc gacgatctgg
     1921 tacttcacct gctcctcggt gtcctcgtcg acgagcgtca ccgtcgcgcc gaacttcacc
     1981 gtgtcgccgc tgagtgtgct cggatcgatg acctcggcac gcgagagctt gtcctcgagc
     2041 tcggccacct tggcctcgtt catgccctgc gcttccttgg cagcgtgata ctcggcgttc
     2101 tcgctgagat caccatgcga gcgggcctcg gcaatggctt ggatgatgcg cggccgctcc
     2161 accgatttca ggcggtgcag ctcggcctcg agctgcttgt aaccctcgat cgtcatgggg
     2221 acgcgttcgg ttgccatagt caaagtctcc tcaccggcac gaactgcgcc ggagcctcat
     2281 ctgtggacgt tccactcact accgggaaat cggccagccc caattacgca gaagggctga
     2341 tgtggaaaag ccgccctcag gcggcggttc cagcatagcc ctgcagaggc gcgaccttca
     2401 atgtatccga tttcaaggct ctgatggcct tggtgactgc gatcgcacca gccagagttg
     2461 tataataagg aatgtgattc agcaaggccg tgcgccggat atccttggag tcggccagcg
     2521 cccgcgcccc ttccgtggtg ttgaagacga tggcgacctc gccattcttc atggcgtcca
     2581 cgatgtgcgg acggccttcc agcaccttgt tgatgtgctc gcaggggatg ccgtgctgct
     2641 ccaggtgtcg cttggtgcct cttgtcgcga tgatgcggaa gcccatctcg acaaggtcgc
     2701 gcaccggggc cacgacgcga tccttgtcct tgtccttgac cgagacgaaa gccgtccctg
     2761 aggttggcag cgcctgcccc gccccgatct ggctcttgcc gaacgccatg gcgaagtcgc
     2821 tgttgaggcc catgacctca ccggtggagc gcatctcggg gccgaggatc gggtccaccc
     2881 cgacgaaccg ggcgaagggg aacacggcct ccttgacgcc gatgtggtcg agcttgcgcg
     2941 gcttcaaccc gaacgaggcc aatggcttac ctgccatgac ctcagccgcg atgctggcga
     3001 tcggcagccc gatcaccttc gcgacgaacg gcacggtccg gctcgcccgc ggattgacct
     3061 ccaggatgta gacgacaccg tccttgatcg cgaactgaac gttcatcagc ccgaccacgc
     3121 cgagcgcgag cgccagctcc cgcgtctgcc gctccaactc cgcgatggtc gcctcgtcga
     3181 gcgaatgggg cggcagcgag caggccgagt cgccggagtg gatgccggct tcctcgatgt
     3241 gctccatgac gccggcaacg aaggtgtcct tgccgtcggc gatgcagtcg acgtcgacct
     3301 cgatggcgtc ggacagatag cgatcgatca gaagcggacg ccgctcggac acgacgagct
     3361 cggacggccg gtcgagcgtc gccgacagcc gggcgacgta gcggtcgaga tcggcgccgt
     3421 cgtggacgat ctccatggcg cggccgccga gcacataaga gggccggatc atcacgggat
     3481 agccgatcgc atccgccacc gaacgcgcct ccgagggcga gcgggcgatg ccgctttcgg
     3541 tttgcctcag cccgaggcgg tcgatgagcg ccttgaagcg gtccctgtcc tccgcaaggt
     3601 cgatcgcatc gggcgaggtg ccgagaatgg gcacgcccgc ctcttccagc gcgcccgcca
     3661 gcttcagcgg cgtctggccg ccaaactgca cgatgacacc cttgagcttg ccgtttgcct
     3721 gctcggtgcg gatgatctcc agcacgtcct cggccgtcag cggctcgaaa taaagccggt
     3781 cggacgtgtc gtagtcggtc gagaccgtct ccgggttgca gttgaccatg atggtctcgt
     3841 agcccgcctt ggtcagcgcg aagcaggcat ggcagcagca gtagtcgaac tcgatgccct
     3901 gcccgatccg gttcggcccg ccgcccagaa tgatgacctt ctcgcgatct gaaggttgcg
     3961 cctcgcattc gggcttgccg tcgagaccgc gctcgtaggt ggagtacatg taggccgtcg
     4021 gcgaggcgaa ttcggccgca caggtgtcga tccgcttgta gaccggctcg acgccgagct
     4081 ccttccggcg ccggcgcaca tcggcttccg tggtgccggc cagcttggcg agacgcgcgt
     4141 cggagaagcc catcgccttc agatcgcgaa gctgctcggc ggtcggcggc agcccgtaag
     4201 ccgcgacccg cgcctccgcg tcgacgatct cttgcagccg ctcgatgaac cacgggtcga
     4261 ttttgcagga ggcgtgcacc tgctcatgat cgagcccaag ccgcagcgct tgcgcaacct
     4321 tcaggatgcg atcgggcgaa ggcgtgccga gggcagcacg gatgacattc tcgtcgtcgc
     4381 cctgccccag cccctcgaat tcgatatcgt cgaggcccgt gaggcccgtc tccatcgagc
     4441 gcagcgcctt ctgcaagctt tcggcgaagg ttcggccgat ggccatcacc tcgccgacgg
     4501 acttcatcga cgtggtgagc gtcgcgtcgg cgccagggaa cttttcgaag gcaaaacggg
     4561 ggatcttggt gacgatgtag tcgatcgtcg gctcgaatgc cgccggcgtg gcgccgcccg
     4621 tgatgtcgtt gtcgagctcg tccagggtgt agccgaccgc gagcttggcc gcgaccttgg
     4681 cgatgggaaa gccggtcgcc ttggacgcga gcgcggagga gcgcgacacg cgcgggttca
     4741 tctcgatgat gacgaggcgg ccgtcggcag ggttcaccgc gaactgcacg ttggagccgc
     4801 ccgtctcgac gccgatctcg cgcagcaccg cgatcgaggc gttgcgcatg atctggtatt
     4861 ccttgtcggt cagcgtcagt gccggggcaa cggtgatcga gtcgcccgtg tgcacaccca
     4921 tggggtcgac gttctcgatg gagcacacga tgatgcagtt gtccgccttg tcgcggacca
     4981 cctccatctc gtactccttc cagccgagca cactctcctc gaccaacacc tgatgggtcg
     5041 gcgaggcatc gagcccccgc tcgatgatct cgacgaactc ctcccggttg taggcaacgc
     5101 cgccgccggt gccgccgagc gtgaacgagg gtcgaatgat ggccggcagc cgcacgtatt
     5161 ccagtgcggc catcgcctcg ctcatggagt gggccagcat cgaccggggt gtctccaggc
     5221 cgattttgcc catggcctcg cgaaagagct ggcggtcctc cgccttgtcg atggcgtcag
     5281 cacgcgctcc gatcatctcg acgccgtact cggcgagaac accttgtttg tgcagtgcga
     5341 gagccgtgtt gagcgccgtc tgcccgccca tggtcggcag cagcgcatcc gggcgctcct
     5401 tcgcgatgat cttggcgacg atctccggcg tgatcggctc gacgtaggtg gcatcggcca
     5461 gctccgggtc ggtcatgatc gtcgccggat tggagttgac caggatgatc cgatagcctt
     5521 cggctttcag cgccttgcac gcttgcgttc cggaatagtc gaactcgcag gcctgtccga
     5581 tgacaatggg ccccgcgccg atgatcagga tggacttgat gtcggtgcgt ttgggcatag
     5641 agaatccgtt gttggctcag gcccgcgcat gcgcggcctc gccaaccggc gagcgttgcg
     5701 caggggacgc gatttgaggc taggcgggcc ttatagcaag gttggcgggc gacggaaccc
     5761 cgaaaagtga tcgcaaccag cgcgccgatc gacgcggatc agactatcgc cagacgcgta
     5821 tatcgcgcgg ggcttgtgag ctgcgcattt ttcgcgcagg ctattcccag cgtgtcccgc
     5881 cgacgcgagt gatttcgcga gacggctata gcgccggtac gcggcccggc ccgaccaagg
     5941 tacggtcgag cgagcaaaag cgttcttccg gcggaggagt ggaacgcgcg aggtaaggtc
     6001 atgtcgatcg aggacgaaat caggaccttg cagcagatcc cgatgttccg ggacctggag
     6061 gtcagcaagc tcaagctgat ggcgtttgcg ggccagagga tcacctgccg caggggcgag
     6121 ctgctcttcg acgagggcga cccgcccgat gcggtctacg tcatcctcga gggtgaagtg
     6181 gaggtgatac gcaccggtgc ggccggcgag gtcgtgctgg cccggctcgg caagaacgag
     6241 ctgatcggtg agatcggcgt gctgtgcaac aagtgccgca acgcccgcat catcgcccag
     6301 tcggatgtcc aggccctgcg catcgacaag atcacgttca tgcagatcgt gcagcaggtg
     6361 cctcagctcg ccatggcaat cattcgtgag ctcagcgagc ggctcgagca caccagcgag
     6421 aagctggccg cgcgtggatg agcgggtcgc cgctttggca ggaggcgcca atagacagta
     6481 ggtgccaata gaaaagggtg cctcgtcggg gggagaccaa gaggcaccct ggaccaccaa
     6541 tcgaattagg gggcatgacc agtggtcgaa cgctgaacgt
//
LOCUS       DASVDU010000169         8609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_698507, whole genome shotgun sequence.
ACCESSION   DASVDU010000169 DASVDU010000000
VERSION     DASVDU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698507"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1319)
                     /locus_tag="VF226_10675"
     CDS             complement(18..1319)
                     /locus_tag="VF226_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7074493.1"
                     /translation="MSAPLIGILGIVALLVLIALRIPVAIAMGVVGLVGGVALNGWFS
                     IGFVLGSQPFVTTSSYSLSVIPLFVMMGAFAARAGLSKSLYQALHALIGHWRGGLASA
                     TIGACALFGSVCGSSLATAATMARVAVPEMTAIGYDRRLTSGALAAGGTLGILIPPSI
                     IMVIYALLTEQSIGRMFLAGFIPGILAALLYISVVYGIARLKPSWAPAAPLGTRTSKR
                     DALYSMAPILALFTVVMGGIYVGIFSPTEAAGVGAFGAIVLAALRKRLNWAVAREALI
                     ETAATTGMLFMILIGTSVLQFFIETSTLPRVIVDWIGALGLPPVGVIVLIIIVYIILG
                     CFLDSLSMMLITLPILFPVVMDLGYDPIWFGVLVVSVVEIGLITPPLGMNLFVITASV
                     ENLRFETVAIGALPFIAADMVRLTLLVAFPSLSLALPALLM"
     gene            complement(1325..1792)
                     /locus_tag="VF226_10680"
     CDS             complement(1325..1792)
                     /locus_tag="VF226_10680"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7074494.1"
                     /translation="MTRSTLFLALVGTGAFLIAVALTVIDIALRSVSTLTVYGLTDIV
                     TLCTMAGAMLALPYGFAADQHVSIDVFTSRMPPAIRRWLAVLAHLLGFAFLSGVFWFG
                     SQQMLIEYGYGDRSQSIGIPMVWYWVPLLIGIGLSALINVWLLIRALRADDQV"
     gene            complement(1856..2860)
                     /locus_tag="VF226_10685"
     CDS             complement(1856..2860)
                     /locus_tag="VF226_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7074495.1"
                     /translation="MKRMAATLALVGGMAWTVPGMAAEFELKISTMFPSTHFIQTLAM
                     EPWAKEIEERTNGRVKFTFFPAGSSMGDATKQFDQARAGVVDVSVGIPAIPRNRHPRS
                     VLVELPFTVPNSEVGTCALMRVKDDLAPDYPGTHILYLTITEPSAAHTRKKIQSLDDL
                     KGMRVRTPTPAITAMLNEIGATPVGMPPTEIYESVERGVVDGNIMPWGPVGAFKLWEV
                     LKYHLDARINPVAMYIVMNQRRYEGLPEDVRKVIDEVSEELFANWGKWWRETDQEAID
                     AAKANGNEVIPMSDEDREAWRKRLTPVIDNYLEKDSGLGADEAKALYAKLRQAVADCE
                     "
     gene            complement(2890..4212)
                     /locus_tag="VF226_10690"
     CDS             complement(2890..4212)
                     /locus_tag="VF226_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7074496.1"
                     /translation="MHEPEIETLPWADQRRLDDELYRAQIAYLLERSRFYQRKLREAG
                     FDSPQKIGGLDNISALPLTEKDELRASRSEADPIGTHLAADMHELVRIYSTSGTTGTP
                     SYIPLTASDLENWVRTSARSYAASGVKAGDRMITTYNAGPFVAGAALDAFERLGLCHI
                     PVGTGNTERLMTAVALLKPQALTCTPSYALHLAEWGQERGMDLPASSIERILVAGEPG
                     GGEPAMRAKLEAAWGAKVTEAMGIGDIGASLWGECEEQAGMHFCARGFVHFELIDPET
                     QQPVPLEDGARGELVYTHLAHRGAPMLRFRSRDHVVVNMQPCRCGRTSPRVRCIGRTD
                     DMLIVRGVNVFPSAIREVVNEFAPDVSGVISVRPSRRGVQQSPPLKVLVELAENGNSD
                     GLSERIRQRLRDKLIVTTDIKLVPQGSLPRSEYKSRLVDYTEASDETA"
     gene            complement(4212..5006)
                     /locus_tag="VF226_10695"
     CDS             complement(4212..5006)
                     /locus_tag="VF226_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HEX7074497.1"
                     /translation="MLIGKHGKATSAICTADADTITIRGRDLCRDLMGRMTFTEFFYL
                     LVTGREPTENQRYFLDLLLIAIAEHGLTPTAVAARMTYDADPQSLQSAVAAGILGCGT
                     VVLGTSELCGKLLAEAKARVDAGEDPASAAKAIARATHAAGGKLPGFGHPLHRPVDPR
                     AQRILELAEERKVSGQHVALARAFESAAAEVWGKPLTMNVSMPIAAVLMDLGFPPGLI
                     KAIPLLARTGGLLAHLAEEQANPIGFLMASHAEAAISYEAGEEGGS"
     gene            5168..5875
                     /locus_tag="VF226_10700"
     CDS             5168..5875
                     /locus_tag="VF226_10700"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7074498.1"
                     /translation="MDKDNAASVATGRGGAVTGRGGTVERLVQRLRDGIIRSQYAPGQ
                     RLIEADLTRDFGVSRGPLREAFRRLSAEGLVQIVPHRGAVVRRLSFSETMELFQIRTA
                     LECLAVQLAADGIDTGDNRARFEDAIRPIWNESPRASGATYHEENRRFHQAILDICGN
                     QQLAELSRQLQLPLLMLQMSGVMTSDMYADSVIEHRAIASAILAGDAKRAERAMRKHL
                     NRAQAIARAMPKSVFRS"
     gene            complement(5961..6653)
                     /locus_tag="VF226_10705"
     CDS             complement(5961..6653)
                     /locus_tag="VF226_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEX7074499.1"
                     /translation="MGKLKAIEEALEEMLSNLSPGTRLPPERTLSRELGISRGTLRQA
                     LERLEAKGKLWRHVGQGTFVGARPAGSDARLSVVSQATSPHELMEMRLILEPQIARFA
                     ALRATEQEIAHMRHCVRKTGVVTESQAYELWDATLHRAVAQAAHNTLLLVVFDAVNEV
                     RSITEWGRLRDMVVRSREVQLTWWRQHEEFVEAIANRDAPRAEEAAREHVETVLRQML
                     DVSKQLLRSRVA"
     gene            6970..8058
                     /gene="dctP"
                     /locus_tag="VF226_10710"
     CDS             6970..8058
                     /gene="dctP"
                     /locus_tag="VF226_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7074500.1"
                     /translation="MKRRSFLKAGLVGGAAATVAAPAVVRAQETFRWRMTNAYAPGAP
                     FYTTGPGSPDDFAKRVEQMSGGRLKIQHFGAGELIPALEGFDAVRSGTVEMNAANAYF
                     WAGKSFAAQYFTTVPFGLNFAGQNAWLYHGGGLELWHELYKDFGLVAFPLNNTGVQMT
                     GWFREPINAISDLDGLKMRIPGLAGQVYAALGVNVKLLPGGEIFPALERGVIDAAEFV
                     GPYQDRRMGLHNAAKYYYTTGWHEPQNTGELLINKAAWEKLPADLQMIVKVAAQSTTL
                     ESYAWSEAVNAEALADLVEKEGVKAQVLPAEIVNRMREVTREILEQKAAEDPVVKKVH
                     EAFFAFKDKWERWSAVSESVYYGQVLKS"
     gene            8176..>8609
                     /locus_tag="VF226_10715"
     CDS             8176..>8609
                     /locus_tag="VF226_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7074501.1"
                     /translation="MEGHNQTADPPETYGGDLAGRIARFIEAIVEWAGRLMMVLVFAI
                     VMLVATNVILRYLFAIGPVSLQELEWHLMVPVALIGSAYTLRHHGHVRVDIFYDGFGA
                     RTRAVVNLFSSIGLLVTSVLVVWLSLNFVNQAYVIDEGSPDPG"
BASE COUNT         1736 a         2611 c         2634 g         1628 t
ORIGIN      
        1 gctcagcgag tgccgtgcta cataagcagc gcaggcaggg ccaatgataa cgacgggaac
       61 gcaaccagca gagtaagccg caccatgtct gccgcaatga acggcaacgc gccaattgcc
      121 accgtctcaa agcgcaggtt ctcgaccgaa gctgttatga cgaacaggtt catgccgaga
      181 ggcggggtga taagcccgat ctcgacaacc gacacgacga gaacgccgaa ccagatcgga
      241 tcgtagccga ggtccatgac caccgggaac agaatcggca gagtgatgag catcatcgac
      301 aggctgtcga ggaaacagcc caagatgatg tagacaatga tgatcaggac gatgacgccg
      361 accggcggca gccctagcgc cccgatccaa tccacgatga cgcgcggcag ggtggatgtc
      421 tcgatgaaaa actgcagcac cgacgtgccg atgaggatca tgaacaacat gccggtggtg
      481 gctgccgttt cgatgagcgc ctcccgagcc acggcccagt tcaagcgctt gcgcagggca
      541 gcaagaacga tcgctccgaa tgcgcccacg cctgcggctt ccgtgggaga gaatatgcca
      601 acgtagatgc cgcccatgac gacggtgaac aaggccagga tcggcgccat gctgtaaagc
      661 gcgtcgcgct ttgatgttcg ggtgccgagt ggtgctgcgg gtgcccagga tggcttcagc
      721 cgtgcgatcc cgtaaacgac ggagatgtag agcaaggccg ccagaatgcc gggaatgaac
      781 cctgccagaa acatgcgccc gatcgattgc tccgtcagca atgcatagat caccatgatg
      841 atcgatgggg gaatcagaat gccgagggtg ccgccggccg ctagcgcacc ggacgtaagc
      901 cgacggtcat agccgattgc ggtcatctcc ggcacggcaa cgcgggccat cgtggccgca
      961 gttgccaggc tcgaaccgca gaccgaaccg aacaaggcac aggcgccgat ggttgcggag
     1021 gcgagcccac cgcgccagtg cccgatgagg gcatgcagag cctggtaaag ggatttcgac
     1081 aggccggcgc gcgcggcgaa cgcgcccatc atcacgaaca ggggaatgac cgacagggaa
     1141 tagctcgatg tcgtcacgaa cggctgcgag ccaagcacga agccgatgct gaaccagccg
     1201 ttcagcgcca caccgccaac caggccgaca actcccatgg cgatggccac ggggatccgc
     1261 aaggcaatca ggaccaggag cgcaacgatg cctaggatgc cgattagggg tgccgacatc
     1321 gaactcagac ctgatcgtcg gcgcgcaatg cgcggatgag gagccagacg ttgatcagcg
     1381 cacttagccc gatgccaatc agcaggggca cccagtacca gaccatcgga atgccgatcg
     1441 actggctacg gtcaccgtag ccgtattcga tcagcatctg ttgcgatccg aaccagaata
     1501 cgcctgacag aaaggcgaag ccgagcagat gcgcaagaac tgccagccag cgccgtatcg
     1561 ccggtggcat gcggctggta aacacatcga tcgagacatg ctgatcagca gcaaaaccgt
     1621 aggggagggc caacatggcg ccagccatgg tgcatagcgt gacaatatcg gtcagtccgt
     1681 agaccgtcag tgtgctcacc gatctgagcg cgatgtcgat gactgtgagc gccaccgcga
     1741 tcagaaatgc gccggttccg acaagcgcga gaaacaaggt tgagcgtgtc acggaggaaa
     1801 tctcgaccga ggaacggagg cggcaggcca aaggcgccta ccgccccagg tttgactatt
     1861 cgcagtcggc tactgcctgt cgcagctttg catagagcgc cttggcctcg tctgcaccga
     1921 gaccggagtc cttctcgagg tagttgtcga tcaccggtgt cagtcgcttc cgccaggcct
     1981 cacgatcctc atccgacatg gggatgacct cgttgccgtt ggcctttgcg gcatcgatag
     2041 cctcctggtc ggtctcgcgc caccacttgc cccagttggc aaagagctcc tcgctcacct
     2101 catcgatgac cttgcgcacg tcttccggca acccttcgta ccggcgttga ttcatgacga
     2161 tgtacatggc gaccggattg atgcgcgcat caaggtggta cttgaggact tcccaaagct
     2221 tgaatgcacc gaccggtccc cacggcatga tattgccgtc cacgactccg cgctcgacac
     2281 tctcgtagat ttctgtcggc ggcatgccga cgggcgttgc tccgatctca ttcagcattg
     2341 ccgtgatggc gggcgttggc gtccgcacgc gcatgccctt caaatcgtcg aggctctgga
     2401 tcttcttgcg cgtatgtgcg gcgctcggct cggtgatggt gaggtaaagg atgtgggtgc
     2461 cgggatagtc cggcgcgaga tcgtccttga cccgcatcag cgcacaagtg ccgacttcgg
     2521 aattgggcac ggtgaatggt agttcgacca gcacagaacg cgggtgccgg ttacgaggaa
     2581 tagcggggat gccaacagaa acatcgacga caccggcgcg cgcctgatcg aattgcttcg
     2641 tggcgtcgcc catggaggag ccggccggga agaacgtgaa tttcacacgc ccattcgtgc
     2701 gctcctcaat ctcttttgcc cagggctcca tcgcaagtgt ctggatgaag tgcgtggacg
     2761 ggaacatcgt ggaaatcttg agctcgaact cggcggccat gccgggcacg gtccaggcca
     2821 tcccccctac gagggcgagt gtggctgcca tcctcttcat tgcgcttgct cctccaaaaa
     2881 gatttttctt taggccgttt cgtccgaagc ctctgtgtaa tcgacaagcc tggacttgta
     2941 ttcgctgcgc ggcaacgatc cttgcgggac cagttttatg tccgtcgtaa cgatgagctt
     3001 gtcgcgcagc ctttgccgga tgcgttctga aaggccatca gaattcccgt tttccgcgag
     3061 ctcgaccagc accttgaggg gcgggctctg ctgcacgccc ctgcggctgg gtcggacgga
     3121 gatgacgccg ctgacatccg gggcaaattc gttcaccact tcgcggatgg cggatgggaa
     3181 gacgttcacg cctcggacga tcagcatgtc atccgtgcgg ccaatgcagc gaacccgcgg
     3241 tgatgtccgg ccgcagcggc agggctgcat gttgacgacg acatggtcgc gggagcggaa
     3301 ccgcagcatc ggcgcaccgc gatgggcgag atgggtgtag acgagctcac ccctcgcgcc
     3361 gtcctctaac ggaacaggtt gttgcgtttc ggggtcgatc agctcgaagt gtacgaaccc
     3421 gcgagcgcag aagtgcatgc ccgcctgttc ctcacattct ccccagagcg aagcgccgat
     3481 atcgccgatg cccatcgctt ccgtcacctt ggcgccccag gcagcttcga gctttgcgcg
     3541 catcgccggt tcgccgccgc cgggttctcc cgcaacgaga atgcgttcga tgctgctggc
     3601 cggcaggtcc atcccgcgtt cctggcccca ctcggccagg tgcagcgcgt aggacggcgt
     3661 gcaggtcagg gcctgcggtt tcaggagcgc cacggccgtc atcagccgtt cagtgttgcc
     3721 ggtgccaacc gggatatggc agagtccaag gcgctcgaac gcatcaagtg ccgcgccggc
     3781 gacgaatggc ccggcattgt aggtggtgat catccgatca ccagccttta cgccggaggc
     3841 cgcgtagctg cgcgccgacg tgcgcaccca attctccaaa tcactggcgg tcaagggaat
     3901 gtagcttggc gtacccgtcg ttccgctcgt cgaatagatg cggacgagct cgtgcatgtc
     3961 tgccgcgaga tgcgtgccga tggggtctgc ctcacttcgc gacgcccgga gctcgtcttt
     4021 ctctgtcaat ggcagggcag agatattatc gaggccgccg atcttctgtg ggctatcaaa
     4081 acccgcttcg cggagcttcc gctgatagaa gcgggaccgt tccagaagat aggctatttg
     4141 ggcacggtaa agttcatcgt cgaggcgacg ctggtcggcc cacggcagag tctcgatctc
     4201 cggctcgtgc atcaagatcc gccctcctca ccagcctcgt aggaaatggc tgcctcggca
     4261 tgagaggcca tcaggaaacc gatcgggttt gcttgctctt cggcgagatg ggcaaggagg
     4321 ccgcccgttc tggcgagaag cggaatggcc ttgattaatc ctggcggaaa accgaggtcc
     4381 atcagcacgg ccgcaattgg catcgagaca ttcatcgtca gcggcttgcc ccagacctcg
     4441 gcagcggcgg attcgaacgc gcgcgcaagc gccacatgct ggccagacac tttgcgctcc
     4501 tcggcaagct cgaggatccg ctgcgcccgc ggatcgacgg ggcgatgaag cggatggccg
     4561 aacccgggca gcttgccccc cgcagcatgt gtcgcgcggg ctatcgcttt cgccgcactt
     4621 gccggatcct ccccggcatc gacgcgggcc tttgcctctg ccaggagctt gccgcacagc
     4681 tccgacgtcc cgagcacgac tgtaccgcag ccgaggattc ctgccgcgac cgccgattgc
     4741 agtgactgcg ggtcggcatc ataggtcatc cgcgctgcta ctgccgtggg agtaagccca
     4801 tgctcggcaa tcgcgatgag gagaagatcg aggaaatagc gctggttttc tgtcggctca
     4861 cgcccggtga ccagcagata aaaaaattcc gtaaacgtca tccgccccat cagatcccga
     4921 cagagatcac ggccacggat ggtgatggtg tcggcgtccg ccgtgcagat ggcggacgtt
     4981 gccttgccgt gcttcccaat gagcactgag cacctcttct gttgcagggc ttgccagctc
     5041 aatgcgaatt caagccagcg gtcctcccgg ccatctttac tcagccttgt tgaagggcaa
     5101 gattgtagga caatccagat tgagtcaaga tatcggctgg agccggacag gccgtaaggg
     5161 accgattatg gacaaggaca atgccgcaag tgtagcgacc ggccgcggcg gagcggtgac
     5221 cggccggggc gggacggttg agcggctcgt gcagcgattg cgggatggga tcatcagaag
     5281 ccaatacgcg cctggccagc ggctgatcga ggccgatctc acgcgcgact tcggcgtgag
     5341 tcgtggtccg ctcagggaag ccttccgccg gttatccgct gaaggattag tgcaaattgt
     5401 gccccaccgc ggggcagtgg tacgccgcct ttccttcagc gagacgatgg aactcttcca
     5461 gatccggacg gcccttgagt gcctcgccgt tcagctggcc gccgacggca tcgacacagg
     5521 tgacaaccgc gcccggtttg aggatgcgat ccgtcccatc tggaatgagt cccctcgcgc
     5581 atcgggcgcc acttaccacg aggagaaccg ccgctttcac caagcgattc tcgacatttg
     5641 cggcaaccag cagctcgccg agctgagccg acaactgcag ttgcctcttt tgatgctgca
     5701 gatgagcggt gtgatgacct ctgatatgta tgcagattcg gtgatcgaac accgtgccat
     5761 tgcgtcagcg atccttgcag gcgacgccaa gcgcgccgaa cgggcaatgc gcaagcatct
     5821 taatcgcgca caggcgatcg caagggcaat gccgaagtcg gtcttcaggt cttgagagcg
     5881 cgccgaatgc tgaaggccga aaaaggatcg gccctcgatt gcgccttcga gaactggaag
     5941 ccaaatgtcc gaatgtcact ttacgcaact cggctccgaa gtaattgttt tgacacgtcg
     6001 agcatctgcc ggagcactgt ttcaacgtgc tcccgcgctg cttcctccgc tctcggcgcg
     6061 tcgcgattgg cgattgcttc gacgaactcc tcgtgctggc gccaccaagt gagctgtacc
     6121 tcacgtgagc ggaccaccat atcccgcagg cgtccccatt cggtgatgga gcggacctcg
     6181 ttgaccgcat cgaagacaac gagcagcagg gtattgtgag ctgcttgggc cacggcccga
     6241 tggagcgtcg cgtcccacag ctcgtaagcc tgcgattccg tcacgacgcc cgtcttgcgc
     6301 acgcaatggc gcatgtgggc gatttcctgt tccgtagctc tcaaagcggc aaagcgcgcg
     6361 atctgcggct cgagaatgag acgcatttcc atgagctcat gcgggcttgt tgcttggctg
     6421 accactgaca gccgggcatc gctgcctgcg ggccgtgcac caacgaacgt gccttgcccg
     6481 acatgccgcc aaagcttgcc cttggcttcg agccgctcga gcgcctgacg cagcgtgccg
     6541 cggctgatcc ctagctcgcg gctgagcgtt cgctccggcg gaaggcgcgt tcctggactg
     6601 agattggaca gcatctcctc gagagcttct tcaatagctt tcagttttcc catatcaatg
     6661 tccgagataa tccaatcact gaattggtac agaaatgtca tctgaacttc aatccgctga
     6721 ctgccttatg aacagtcttg ttgccgcgat ttttggcaga tctgggcaat tctcaaccag
     6781 ctgttctttt gttgtcacca ttcctaaatt ggtctatcgt tgattgaggt gtcaaaagag
     6841 caccgtcaat gacgggtgcg gacgggcacg atgaccggtg aggttcggag cggcaaaagc
     6901 gcttccaaac ctcgatcgtc gtggcaagcc gctccgaccc aagaggaaac tgaagccagg
     6961 gaggagacca tgaagcgtcg ttcctttctc aaggccgggc tcgtcggagg tgcagctgcc
     7021 acagtcgccg cccctgccgt cgtccgcgca caagagacgt tccgctggcg catgaccaac
     7081 gcttacgcgc cgggggcacc cttctatacg accggtccgg gcagcccaga cgatttcgcc
     7141 aagcgcgtcg agcagatgtc tggtggccgc ttgaaaattc agcacttcgg tgcgggcgag
     7201 ctgatcccag cgcttgaggg cttcgacgcc gtgcgctcgg gcacggtcga gatgaacgcg
     7261 gccaacgcct atttctgggc cggcaaaagt tttgccgcgc agtatttcac cacggtgcct
     7321 ttcgggctca atttcgccgg ccagaatgcc tggctttatc acggtggcgg gctcgagctt
     7381 tggcacgagc tttataaaga cttcgggctc gttgccttcc ccctcaacaa caccggcgtg
     7441 cagatgacgg gctggttccg cgaacctatc aacgcgatca gcgatctcga cgggctcaag
     7501 atgcgcattc ctgggcttgc aggccaagtc tacgccgcac tcggagtgaa tgtgaagctg
     7561 ctgcccggtg gcgaaatctt tcctgcgctc gagcgcggtg tcatcgatgc ggccgagttc
     7621 gtcggtccct accaggaccg tcggatggga ctgcataacg ctgccaagta ctattacacg
     7681 accggctggc acgagccgca gaacacgggc gagcttctga tcaacaaggc ggcgtgggaa
     7741 aagctgccgg ctgacctgca gatgattgta aaggtcgcgg ctcagtccac cacactcgaa
     7801 agctatgcct ggtcggaggc cgtgaatgcg gaagcgttgg ccgatctggt agagaaggaa
     7861 ggcgtgaagg ctcaggtcct gcctgccgaa atcgtcaacc gaatgcgcga ggtcactcgc
     7921 gaaattctcg agcagaaagc cgctgaagac ccagtggtca agaaggtgca cgaggccttc
     7981 ttcgctttca aagacaagtg ggagcgctgg tcagcggtca gcgaaagcgt ctactacggt
     8041 caagtcctca agagctaagc ggcaaaaccg gcacgcggag cgtaagcccg cgcagccggt
     8101 gcccatccct gatcagaacc gacgggaagg cgggccggcg ttcatccagc ccgccgcaaa
     8161 gcaatggagg acgccgtgga gggacacaac cagactgccg atccccccga aacttatggt
     8221 ggtgacctcg caggacgaat tgcccgcttc atcgaggcaa tcgtcgagtg ggcgggacgt
     8281 ctcatgatgg tgctggtctt cgccatcgtg atgctggtcg cgacgaacgt tatcttgcga
     8341 tatctgtttg cgatcgggcc ggtttcgctg caggagctcg aatggcatct gatggtccca
     8401 gtggcgctga tcggcagtgc ctatacgctg cgccatcacg gccatgttcg cgtcgacatc
     8461 ttctatgacg gcttcggcgc ccgtacgcgc gccgttgtca acctgttcag ctcgatcggt
     8521 ctcctcgtca cctccgttct cgtcgtctgg ctctctctca acttcgtcaa tcaagcctac
     8581 gtgatcgacg agggctctcc tgatcccgg
//
LOCUS       DASVDU010000170        31883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_591148, whole genome shotgun sequence.
ACCESSION   DASVDU010000170 DASVDU010000000
VERSION     DASVDU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 31883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31883
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591148"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1600)
                     /gene="murJ"
                     /locus_tag="VF226_10720"
     CDS             complement(20..1600)
                     /gene="murJ"
                     /locus_tag="VF226_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7074502.1"
                     /translation="MNLYRAFATVGGLTMMSRLLGFVRDILIAAALGTGFVADAFFVA
                     FRFPNLFRRLFAEGAFNAAFVPLFAKRLEGDGHHSARQFAEEALACLTFVLVLFSAVA
                     IIAMPWLMLLLAPGFTSDPEKYDLAVLLTRIAFPYLLCMSLVALLSGVLNSMARFLAA
                     AAAPIILNVVLIGAISIAIWAGYDAQPGAGMILAVGVTIAGIAQLLMLWIAVSRTGMR
                     LRLTWPHYSEGVKRLVALGIPGLISGGITQINIVVGTIIASLQAGAVSYLYYADRLYQ
                     LPLGIIGVAVGVVLLPDLSRKLRAGDGAAAHDSQNRSLEFALLFTIPAAVALVIAAEP
                     IISVLFQRGAFSAADTDATAAALAAFAIGLPAFVMIKVFQPAFFAREDTRTPMWFASI
                     NLVVNAVGSAVLFFLFQRLGWMPHVGIAIATTMAGWINAGLLMLALMRSGDYRADRRL
                     IRNTWMILLASVVMGLAIAGILAVMNPYFAREAGLLVRGASLAALVAAGVCIYAALII
                     TTGVLRIDQLRRFMRRAA"
     gene            complement(1597..4365)
                     /locus_tag="VF226_10725"
     CDS             complement(1597..4365)
                     /locus_tag="VF226_10725"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEX7074503.1"
                     /translation="MDVTTLAKPPYFDPLEARHALARLATEKAGAGDARPAVLDYCRT
                     LLKHARASAEAQLASDGNGRRCAAGLSQFTDELIRLIYDYAVTHVYCATNPSDAERMA
                     IVATGGFGRGLLAPGSDIDLLFLLPYKQTPWGESVAEYMLYLLWDLGLKVGHATRTID
                     QCIKLALSDFTILTALLDKRLIHGDADLFRQLEARFRAEVVTSAIARPFIEAKMAERD
                     ERHRRSGESRYRVEPNIKDGKGGLRDLHTLHWLAMYVHGEGPGKGLASAGVFTPAEMA
                     TFRRCESFLWSVRCNLHFLTGRAEERLTFDLQPVMAERLGYRSARGLRGVERFMKHYF
                     LVAKDVGDLTTILCSSLEMQQLKSAPRLEQLLNPVTWVTRRRIRRTTGFRIDNERLNI
                     ADQDVFKRDPVNLIRFFAVAEETGSFFHPNAIRVLRSSLRLIDDELRQNPEANRIFLQ
                     LLTSKNNPETTLRRMNEAGVLGRFIPEFGRVVSMMQFNMYHHFTVDEHLLRTVGELSS
                     IEEGRRAKSLPLSTEIIGSIQNRRALYVAAFLHDVGKGRVEDHSKVGAGIARRLCPRL
                     GLSAAETDTVAWLIEHHLEMSNISLSRDLADPKTIRDFAAVVQSPERLKLLLLLTVAD
                     IRAVGPGTWNGWKGQLLRELYWQTEPLVAGGHTRVSRRQRISEAQAQLRTELAGWPPE
                     EVERFIQRHYDDYWLKTDTHKQVEHANLIRRADADAQRLVTSFTSNAFTAITELTIVA
                     PNHPRLLALFAGACAASGANIIGAHISTTRDGLALDTFLLQRELPDDADEARRMARIT
                     RTIDRALKGEVRMAELMAKRKPSEPRITAFTVEPETIINNSLSDEFSVIEVSGRDRPG
                     LLYDLTSTLSDLNLDITSAHITTYGEKAVDVFYVTDLTGKKVTSEQRQAAIRERLAGV
                     LSRTAEAA"
     gene            complement(4569..7277)
                     /gene="mutS"
                     /locus_tag="VF226_10730"
     CDS             complement(4569..7277)
                     /gene="mutS"
                     /locus_tag="VF226_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7074504.1"
                     /translation="MAQYLEIKAANPDSLLWYRMGDFYELFFEDAVVASQALGIVLTK
                     RGKHLGEDIPMCGVPVARADEYLQRLIKQGYRVAVCEQLEDPAEARKRGSKAVVRRDV
                     VRLVTPGTITEDSLLDAKARNYLTSLHVAPRGADDGDQIVALASLDISTGEFELGSVS
                     ISDLSGELARLQPSEIIATDGTFADPRIKTWIEAIGAATTPVPGATFDSMTGERAIKA
                     RLEVADLAAFGTFSRPELAAAAALLKYVELTQIGRQPIIRPPRSTGPANAMVIDAATR
                     ASLELVRGASGGRQGSLLAAIDRTVTGPGARELAARLASPLRDVRTIRTRLDALAYLL
                     ERDRLREDVRSALRSAPDIARAVSRLALQRGGPADLAAVRDGLTVASALGELLRLSAD
                     GIGLPVELASIETRLSRPETRALADGLRAALVETPPVHRRDGGFVREGFRTDLDEARR
                     LRDDSRKVMAALEARYVEETGIRTLKVRHNNVLGYYVEVGAANAKPLMAEPLSAKFRH
                     RQTLANAVRFTTSELAETEGLIASAAERALAIEQEVFAELARAVGELERALCETAAAL
                     AELDHCAGLAELAFEENYVRPEVTEGLEFEIRGGRHPVVEQALRQAKASPFIENDCVL
                     GEGAARQQGFDEDSEGGRIWLVTGPNMAGKSTFLRQNALIALLAQIGSFVPARSARIG
                     VIDRLFSRVGAADDLARGRSTFMVEMVETAAILNQASERSLVILDEIGRGTATFDGLS
                     IAWATVEYLHDVCRCRALFATHYHELTALTGRLPNLVAVTMDVKEWNDEIVFLHKVQP
                     GAADRSYGIQVAKLAGLPPAVVKRAREVLARLEKTDRRQGTSGSLEDLPLFAHEPSRP
                     ASGGRDAPSELELALDKINPDELSPRAALEALYELKSLRRKESQAR"
     gene            7603..8577
                     /locus_tag="VF226_10735"
     CDS             7603..8577
                     /locus_tag="VF226_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074505.1"
                     /translation="MRFAGAGLLALLLFVVVPPVQTAAQQKAKGAGPFATNEQILQWI
                     NDYRYEPKPQRLPEAVKAMSRLGLFRDEDTAGVYVGFMAGVLGSNPRSAEKLITRMYP
                     MPPEDQHAIIKAIAYSGLPDWKGLMGKFVERMPARKTLIDSYLQGKAPVLHDLPLDTG
                     PAPIDTLWGYYFATGSLEPVYRIVGALRWADDKSDLNRLTAGSVAKWTLANNATRDKE
                     LLDFLREQQHMRNHPEKVRKVLKEVVVAAETFETGKIRKDALAAIEELKRTGPQPNNS
                     WAKAAYWGSTAVAVGCVVASALGAAAIGVPCILTGAASSAAVKLLNGP"
     gene            complement(8833..10176)
                     /locus_tag="VF226_10740"
     CDS             complement(8833..10176)
                     /locus_tag="VF226_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7074506.1"
                     /translation="MRKPEFAFWTFLLMAGLAGLTTVANVTQTYSASSSNSEIYRQLD
                     LFGDVLERVRSDYVEKPDDTQLIESAINGMLASLDPHSAYLSPKHFRDMQVQTRGEFG
                     GLGIEVTMENGVVKVVAPIDGTPAQRAGIQANDLITHLDDEQIVGLTLEQAVEKMRGP
                     VNTPIKLTIVRKGRDEPFDVTITRDIIRINPVKARLEADDQVGYVKISTFNEQTHANL
                     VKSVDELKKEAGKKLKGYIIDLRNNPGGLLDQAVAVSDAFLDKGAIVLTKGRNLEETQ
                     RSQARPGDITDGKKIVVLVNGGSASASEIVAGALQDHKRATVIGTRSFGKGSVQTIIP
                     LGSNGAIRLTTARYYTPSGRSIQAQGIHPDIVVEQDLPPELQGKEPAKPRGEASLRGH
                     LKPDKEGVKEESGSSAYVPQEPEKDKQLQYAISLLLGKVAASTESPAATQEKVPN"
     gene            complement(10189..11598)
                     /locus_tag="VF226_10745"
     CDS             complement(10189..11598)
                     /locus_tag="VF226_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7074507.1"
                     /translation="MRARREHAASAARPGGRWCAAGQRATVCLAAAATLLLAASPVVA
                     QSQLATEAKQKLEQDRKRLQEAAEREKQLKADLATMEQERARLNEQLQAAARSVQLSE
                     AQLSSIEERRDALVAQQKMHQEALQQRHSSISSLLAAMQRIGRNPPPVIVTRREDALE
                     MVRSAMLLGRAIPELQAEVQDLVARLQELDRVFKQIAEERAKIEAETERLKAAQARVA
                     MLMEQKRHSLTERQGELAAVRREADRISRSVSNISELITALDQTVAEKTGLATYNKQL
                     PKGADAGQPGSHGKAPDGKAAATAKETEVAVVLSPGSGPLLGPPGRMQPAMPFHLAKG
                     KLPMPAHGRTVVSYGETTSNGRKSQGIVIQTRYGAQVTSPSDGWVVYAGEFRSYGQLL
                     IINAGGGYHVLLAGLSRIDAQLGQFILAGEPVGTMQVAAGGQSDENAPVLYVEFRKEG
                     RPVDPSPWWHQGEQLKVQG"
     gene            11827..12015
                     /locus_tag="VF226_10750"
     CDS             11827..12015
                     /locus_tag="VF226_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEX7074508.1"
                     /translation="MNSLFYFLTILAPVAVLVVLLLGLVNLMRARNANTSQKLMRWRV
                     GLQFVAIVLIMITVYLTH"
     gene            12096..12677
                     /locus_tag="VF226_10755"
     CDS             12096..12677
                     /locus_tag="VF226_10755"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7074509.1"
                     /translation="MVVLNKIYTRTGDDGTTALASGQRVRKDSIRVAAYGTIDETNAA
                     IGMARVHLAGSHPGLVEKLARVQNDLFDLGAELSTPDTGKPKARERMEITPAQVKRLE
                     DDIDELNAHLQPLRSFVLPGGSPGAAALHMARTICRRAERLMVTMAADPNEGVSQVAL
                     QYVNRLSDLLFVASRYVNDRGKSDILWVPGQNR"
     gene            12734..13435
                     /locus_tag="VF226_10760"
     CDS             12734..13435
                     /locus_tag="VF226_10760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7074510.1"
                     /translation="MFIPVYDQNPLRSIQFQAVTTGLILVNIVVFILQITTLSNQVIL
                     GFALVPNDLLGLGAAGQGRLLAPGNEIGIPERLTLISYMFFHGDILHLAGNMLFLWVF
                     GDNVEDALGHLKFLIFYLTCGVVGGLVHALMMPSSEMPLIGASGAVAGVIAAYLMLHP
                     RVRVWVLIFRFIPIQISAAFALGAWIFTQFLMVLLPEVGPVAWWAHIGGIIAGAVLVT
                     VMRRPGVPLFDRNIA"
     gene            13674..14429
                     /locus_tag="VF226_10765"
     CDS             13674..14429
                     /locus_tag="VF226_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7074511.1"
                     /translation="MKILVPVKRVVDFNVKIRVKPDGSGVELANVKMSMNPFDEIAVE
                     EAIRLKEKGIATEIVAVSIGPSKAQETIRTALAMGADRGILIETDEGVAVEPLAVAKL
                     LKAVVADEQPGLVILGKQAIDDDCNQTGQMLAALLGWPQATFASKVVIDNGSALVTRE
                     VDGGLETLKLQLPAIVTTDLRLNEPRYASLPNIMKAKKKPLDVKKAADFGVDIAPRLK
                     VLQTAEPPARKSGVKVSSVAELVEKLKTEAGVI"
     gene            14429..15373
                     /locus_tag="VF226_10770"
     CDS             14429..15373
                     /locus_tag="VF226_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7074512.1"
                     /translation="MPALVVAEHNNSTFNPVTAKAVTATAALGSEIHVLVAGSNCRGV
                     AEQAAKLQGVTKVLVADAPHLEHQLAEDMTALVVPLMASYEALVAAATSFGKNLLPRV
                     AAMLDVMQISDITAVKSPDTFERPIYAGNAIQTVQSGEAKKVITIRAASFAAAPEGGS
                     ATIEAISVPEATGLSKFERAEIAKSDRPELTAARIVISGGRGMQSSENFKKYLEPVAD
                     RLGAAMGASRAAVDAGYAPNDLQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDGEAPIFQVADYGLVADLFQALPELDAELQKAGY"
     gene            15463..16338
                     /locus_tag="VF226_10775"
     CDS             15463..16338
                     /locus_tag="VF226_10775"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013650881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7074513.1"
                     /translation="MAIKKVGVIGAGQMGSGIAHVVALAGYDVAITDIAKEVVDKAMS
                     NIDRNMDRQVARGLITADQKAAALKRIRYVPDYDGLQDCDLVIEAATEDEAVKRKIFT
                     ELCPKLKPDAIVASNTSSISITRLASITKRAERFIGMHFMNPVPVMQLVELIRGIATE
                     DDTFATARSFVESLGKTIAVSEDFPAFIVNRILLPMINEAVYTLYEGVGTVEAIDKAM
                     RLGANHPMGPLELADFIGLDTCLSVMQVLYEGLADSKYRPCPLLVKYVEAGWLGRKTQ
                     RGFYDYRGEKPVPTR"
     gene            16577..17104
                     /locus_tag="VF226_10780"
     CDS             16577..17104
                     /locus_tag="VF226_10780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013844912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX7074514.1"
                     /translation="MTGDLDKEYRPCVGIMVVNRQGLVWIGRRADAPNEPEGPGSWWQ
                     MPQGGIDPDEDPRIAALRELREETGLHTVKVIAEHPEWLRYDLPPNLIGKAWGGRWRG
                     QRQKWFLVRFLGDDSEVNLTPPPGHQVEFDCWRWSPLDELQDAIVPFKRDVYAKVITA
                     FRPLIGPDTGSRCQS"
     gene            17101..17619
                     /locus_tag="VF226_10785"
     CDS             17101..17619
                     /locus_tag="VF226_10785"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEX7074515.1"
                     /translation="MTFKTGAGRQKLPLRRTVGILLINRSGKVWIGQRRPRWLPPDAE
                     PIWQMPQGGIRAGEKPRAAALRELAEETGITSVEVLAKSRSWITWQLPDELIGVALKG
                     RYRGQRQRWFAMRFVGDDREVSLTPPDGTKPEFDDWRWAKPEEVIALAIPFRRPVYAA
                     VIKEFASLLQKE"
     gene            complement(17666..18067)
                     /locus_tag="VF226_10790"
     CDS             complement(17666..18067)
                     /locus_tag="VF226_10790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX7074516.1"
                     /translation="MADTFKFELVSPERVLLSEDAEQVVLPGVQGDFAVLANHAPMLS
                     MLRPGVLDVQLPSGTRRVFVKGGFAEVEPDRLTVLAQTAFDADASSAQSAIEAELTAA
                     QAELDEAADDDARFMAQEAVDRLRALQSGGR"
     gene            complement(18119..18385)
                     /locus_tag="VF226_10795"
     CDS             complement(18119..18385)
                     /locus_tag="VF226_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074517.1"
                     /translation="MAEAEKTASGGFGTCCDALKEAMAGEDFEPLVSVGRDGVLYLAV
                     GLIELDDNEPGTVDHPVYFCPFCGTQLQTEEEVQAKAEEDPGDE"
     gene            complement(18408..19841)
                     /gene="atpD"
                     /locus_tag="VF226_10800"
     CDS             complement(18408..19841)
                     /gene="atpD"
                     /locus_tag="VF226_10800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7074518.1"
                     /translation="MASNKVGRIRQVMGAVVDVQFEDELPAILNALETTNHGNRLVLE
                     VAQHLGENTVRTIAMDSTEGLVRGQEVTDTGRAIAVPVGEATLGRIMNVIGEPVDEAG
                     PIQGVEQRPIHQPAPEYVEQSTEAQILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTV
                     LIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVNKDPRKHGSAAGSKCAL
                     VYGQMNEPPGARARVGLTGLTVAEYFRDQGQDVLFFVDNIFRFTQAGSEVSALLGRIP
                     SAVGYQPTLATDMGALQERITTTQKGSITSVQAIYVPADDLTDPAPATSFAHLDATTV
                     LSRAIAEKGIYPAVDPLDSTSRMLDPRIVGEEHYAVARQVQAILQRYKSLQDIIAILG
                     MDELSEEDKMIVARARKIERFLSQPFHVAEVFTGSPGKLVSLQDTIKGFKGLCNGDYD
                     HLPEQAFYMVGTIEEAIAKAEQLAEAA"
     gene            complement(20133..21020)
                     /locus_tag="VF226_10805"
     CDS             complement(20133..21020)
                     /locus_tag="VF226_10805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX7074519.1"
                     /translation="MASLKDLRNRIASVKATRKITKAMQMVAAAKLRRAQEAAMAARP
                     YAERMDRILVNLNQRITSREGLSPLLVGTGRDDVHLLVVMTAERGLCGGFNANIARLA
                     RQDIQRLQQEGKTVKILSVGRKGYDILRREYGKLVVDRVDLRGVRQLGYANAQEIADK
                     ILALFEAGEFDVCTLYFAEFKSVIAQKPTAQQLIPAKLPAGEEANAQAGAQAIQEYEP
                     GEEEILEFLLMRNLSTQVFHGLLENAASEQGARMTAMDNATRNAGEMIDRLTLTYNRT
                     RQAMITKELIEIISGAEAL"
     gene            complement(21225..22757)
                     /gene="atpA"
                     /locus_tag="VF226_10810"
     CDS             complement(21225..22757)
                     /gene="atpA"
                     /locus_tag="VF226_10810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7074520.1"
                     /translation="MDIRAAEISAILKEQIKNFGKEAEVSEIGQVLSVGDGIARVYGL
                     DNVQAGEMVEFPNGIRGMALNLEADNVGVVIFGPDSGIKEGDTVKRTGAIVEVPVGPE
                     LLGRVVDALGNPIDGKGPIKATKRMRVDVKAPGILPRKSVHEPMATGLKAIDALIPIG
                     RGQRELIIGDRQTGKTAIILDTFLNQKPLNQGDDEKAKLYCVYVAVGQKRSTVAQFVK
                     VLEERGALEYSIIVAATASDPAPMQYLAPFAGCTMGEYFRDNGLHAVIAYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDEHGAGSLTALPVIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSAAQTKAMKQVAGRIKG
                     ELAQYREMAAFAQFGSDLDATTQRLLARGARLTELLKQPQFSPLKMEEQVIVIFAGTR
                     GYLDPLPVTAVGRFEQELLRTIRDQHADILETIRTEKQLSAETEKQLVAAIDKFAKAF
                     TP"
     gene            complement(22757..23317)
                     /locus_tag="VF226_10815"
     CDS             complement(22757..23317)
                     /locus_tag="VF226_10815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX7074521.1"
                     /translation="MATDEPIMASVAGRYASALFELAEEQRQLAEVEQDLIEFQKLLD
                     DSEDLRRLVRSPVFSSDEQLAAISAVLERVGIGGLAANFLKLAARNRRLFAVSDMIRA
                     FRTMCAKARGEVEADVASAFPLKDDQMKALKDALRASVGKDVQINLRVDPALLGGLVV
                     KIGSRMIDSSLRTKLNSLKIRMKEVG"
     gene            complement(23596..25812)
                     /locus_tag="VF226_10820"
     CDS             complement(23596..25812)
                     /locus_tag="VF226_10820"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7074522.1"
                     /translation="MTSLLELNDEACGALRRLPVVIPVALDQSYDYLAPEKLDIQPGS
                     FVLVPFGPQLRLGVVWDRVVGPERPVTASKLKPLLEVLDVPPLPTLSLRFAEWIARYT
                     LSPLGMVVRMMMGAQAVFEPPRPRFGVRIVEGAPEPPRMTPARKRAIEIASDGLARAK
                     SALAAEAGCTTGVIDGLVQAGVLVEVAIPEKRYPQPWPAHSMVQFGPDQERAVHALRS
                     AVDADNFSVTLLDGVTGSGKTEVYYEAVARALERNRQVLIMLPEIALTSQFLSRFASR
                     FGCPPVEWHSALASAERGRVWRATATGEARVVVGARSALFLPYRELGLIIVDEEHDQG
                     YKQDDRVHYQARDMAVVRGSLGKCPVVLASATASIETYVNARTGRYRHLVLPGRYSGT
                     ELPQVEAIDLRKHPPEKGRWLSPVLVEAIAETAAKGQQTLLFLNRRGYAPLTLCRTCG
                     TRIECPQCSAWLVEHRFRRKLTCHHCGFALPIPGKCPKCGDSESLVACGPGVERIAEE
                     VSERFPTARLAILSSDLIPGLAEMRKVIAAIENREADIVIGTQIVAKGHHFPDLATVG
                     VVDGDLGLGQADPRAAERTFQLLHQVTGRAGRSFAQGRGLVQTHMPDHPVMQAIISGD
                     REAFLDREISQRQAAMLPPFGRLAALIISARHKETAEVYAREVVRRAPLAERIEVLGP
                     AEAPLAVIRGRHRWRILVKAAREVDLQAYLRAWLSAAPDPKGDLRLTVDVDPYNFM"
     gene            25899..26555
                     /gene="fsa"
                     /locus_tag="VF226_10825"
     CDS             25899..26555
                     /gene="fsa"
                     /locus_tag="VF226_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX7074523.1"
                     /translation="MKFFVDTADVAVIKELAATGLLDGVTTNPSLVAKVGRDFKSVVR
                     EICEIVPGPVSAEVAATDYEGMVAEGRVLAAIAKNVCIKVPLTWDGLKACRTLTSDGH
                     MVNVTLCFSANQALLAAKAGATFVSPFIGRLDDIGLDGMELIREIRAIFDNYSELTTD
                     ILAASIRTVNHVRQAALIGADVATIPPAVLQALVKHPLTDIGLAQFVADWEKTGQKIL
                     "
     gene            complement(26824..28509)
                     /locus_tag="VF226_10830"
     CDS             complement(26824..28509)
                     /locus_tag="VF226_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074524.1"
                     /translation="MSLYESLELFTGAGVLALMPALGCRVPPGAALSGGEFSAFLRSR
                     GEGGPIGDVLDRLAHQIWLAQDTRGIPMHTAAEHARRLVPVIDACRPDPEFVNAALRG
                     QHGTPAAAASQIAAQLVAQAKALGPEGFDGLSDDVCNFLLEQLFLQVLADQRLLRDAL
                     PAASEFLGKQQPEPAAASEPGTELTVAEASPESEMAALKERYGLSDRALQRFSAIAEK
                     QRTLPAREAWQVAELAQWLKETREQLLRPTNEPAEIRALKAKAAEALVNGDFEAAMEL
                     LKEVRRSFRAARRRTEQRLNEELEQLRAEIQQEAMATADVAELAMARMDFQSAAELFE
                     EAAESVARTDPAAQLRFTLRRADALYYAGEETSNIPLLRQAVNVYAKAAELAGSNGEA
                     KGLASASQGLGNALLAIAEHEPSKALFEQASIAFRTALEVLSRETDPRIWGLARIGLG
                     NALASMCEVGNASALTLEQAAVAYRGALEVFSRESEPMRWAVTRLNMSTVLIRLGELK
                     DRRENWLAAAAAIVPALEVFEQHGATAQAEAARRALTMLHNRWDLLEPPTAAE"
     gene            28724..29671
                     /locus_tag="VF226_10835"
     CDS             28724..29671
                     /locus_tag="VF226_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEX7074525.1"
                     /translation="MAEGQGELQLSRPLQEAVADWLDHLAGERNYSDLTREAYGRDLR
                     QFLSYWQAQLGRQPSLADLEILEPRTFRGFLASRRRQQASNRSLARSLSALRSFFRWL
                     EGKEIGRSRAVLSVSRPKVPHGVPKPLTVAKAAALIESGIQADPDWVVTRDTAVLLLL
                     YGSGLRISEALGLKVCEAPSKDRDVLRIVGKGGKERVVPVLPITIEAVERYRQQCPYL
                     LEPDDILFRGAKGGPLSPRIIQLAIARARAALGLPETATPHALRHSFATHLLSAGADL
                     RQIQELLGHASLSTTQIYTEVDRARLLAVYDAAHPRARG"
     gene            complement(29682..31079)
                     /gene="lpdA"
                     /locus_tag="VF226_10840"
     CDS             complement(29682..31079)
                     /gene="lpdA"
                     /locus_tag="VF226_10840"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7074526.1"
                     /translation="MTYDLIVIGTGPGGYVCAIRAAQLGMKVAVVEKRPTFGGTCLNI
                     GCMPSKALLHASEAYEEANHAFAAMGIGVKPELDLARMHAFKDEGVKGNVDGVAFLLK
                     KNKIEPFHGTGRITAPGKVEVTADDGAKKQLEAKAIVIATGSDVARLPGIDIDEKTVV
                     SSTGALSLPKVPKKLLVIGAGVIGLELGSVWRRLGSEVLVVEYLDRIVPGLDAEMARN
                     YQRILTKQGFKFKLSSKVTAVNKSNGAHKVTIEPAAGGSAETVSADVVLVCVGRVPYT
                     EGLGLEDVGVQLDNKRRIVVDDHYQTNVPGIYAIGDVIAGPMLAHKAEEEGVAVAEIL
                     AGQAGHVNYGVIPSVIYTFPEVATVGRSEEELKEAGVSFKVGKFPYTANGRAKVNKTT
                     DGLVKVLADAETDRILGVHIIGPNAGELIAEAVVIMEFGGSAEDLARTCHAHPTLSEA
                     VKEAALAVDKRAIHM"
     gene            complement(31103..31375)
                     /locus_tag="VF226_10845"
     CDS             complement(31103..31375)
                     /locus_tag="VF226_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074527.1"
                     /translation="MKAVEPVLYGVLKITWLDGFEAIVDLRPVIADGEVFEFLRKSPE
                     RFNDVKLEEYGHSIFWLDDDGDEVDFGSDSLRRRAIRQAEILRLAS"
     gene            complement(31390..31641)
                     /locus_tag="VF226_10850"
     CDS             complement(31390..31641)
                     /locus_tag="VF226_10850"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HEX7074528.1"
                     /translation="MPTVASVSGVKILFYADEHPPPHFHARIAEFTAQIGIDPVQIMK
                     GSLPPNKESDVLDWARAHRDELIDAWFEMEAGRKPAKIE"
     gene            complement(31655..>31883)
                     /locus_tag="VF226_10855"
     CDS             complement(31655..>31883)
                     /locus_tag="VF226_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX7074529.1"
                     /translation="SLLSTPILNAPQSGILGMHRIEERPVVRDGQIVVRPMMYLALSY
                     DHRIVDGKEAVTFLVRVKELLEDPQRLVLEL"
BASE COUNT         5584 a        10246 c        10250 g         5803 t
ORIGIN      
        1 cggatcgcaa gcccggggat caagctgccc ggcgcatgaa ccggcggagc tggtcaatcc
       61 ggagcactcc ggtggtgatg atcaatgccg cgtagatgca gacacctgct gcgacgagcg
      121 cggccaggct cgcaccgcgc acgagcagac cggcctcacg tgcaaagtag gggttcatta
      181 cggccaggat acccgcgatc gcgagaccca tgacgacgct cgccagcaga atcatccagg
      241 tgttgcgaat cagccggcgg tcggcccggt agtcaccgga ccgcatcagc gccagcatga
      301 gcagcccggc attgatccag ccggccatgg tcgttgcgat ggcgatgccg acgtgcggca
      361 tccagccgag gcgctggaac agaaagaaca gcaccgcgga gcccacggca ttcacgacga
      421 gattgatgga cgcaaaccac atcggcgtgc gcgtatcctc gcgcgcgaag aaggccggct
      481 gaaagacctt gatcatcacg aacgcgggca gcccgatcgc aaaagccgcg agggcggccg
      541 ccgtggcgtc ggtatcggcg gcagagaacg ctccgcgctg gaacagcacg cttatgatcg
      601 gctccgcagc gatcacgagt gcgacggctg ccggaatcgt gaacagcagc gcgaactcca
      661 gcgaccggtt ctgactgtcg tgtgccgcgg caccatcgcc cgcgcgcagc ttgcgcgaca
      721 ggtcgggcag cagcaccact ccgaccgcga caccgataat gcccaggggc aattgataaa
      781 gccggtcggc gtaatagagg tatgagacgg cgcccgcctg gagcgatgcg atgatcgtcc
      841 cgacgacgat gttgatctgc gtgatgcctc cggaaatcag cccaggaatg ccgagcgcca
      901 cgaggcgctt cacaccctcg ctgtagtgcg gccaagtcag tctcaatcgc atccccgtgc
      961 ggctgacggc gatccacagc atcaagagct gggcaatgcc cgcgatcgtg acgccgacgg
     1021 ccaggatcat tccggcgccc ggctgcgcat catagcccgc ccagatggcg atcgagatcg
     1081 ccccgatcag cacgacgttg aggatgatcg gcgccgcggc tgccgccaga aagcgggcca
     1141 tgctgttgag cacgcctgac agcagcgcca ccagcgacat gcataagagg taagggaatg
     1201 cgatccgcgt cagcaggacg gcgaggtcgt atttctccgg atcgctggtg aaacccggcg
     1261 ccagaagcag catcagccag ggcatcgcga tgattgcgac ggccgaaaac aggacgagca
     1321 caaaggtgag gcacgccaga gcttcctcag cgaattgccg tgcggaatgg tggccgtcgc
     1381 cctcaagcct cttggcgaac agcggcacga acgcagcatt gaaagcgcct tccgcgaaaa
     1441 ggcgacggaa caggttcggg aaccggaacg cgacgaagaa ggcgtcggca acgaagcctg
     1501 tgccgagggc agcagcgatc aggatatcgc gaacgaagcc gagcaggcgg ctcatcatcg
     1561 tgaggccgcc gactgtggcg aatgcgcggt aaaggttcat gcagcctcgg cggttcgcga
     1621 caggacgccg gcaagccgtt cgcggatggc cgcctggcgc tgctcgctcg tgaccttctt
     1681 gcccgtcaga tcggtaacgt agaagacgtc caccgccttc tccccgtagg tggtgatgtg
     1741 cgccgaggtg atgtcgagat tgaggtcgga cagcgtgctg gtgaggtcat aaagcagccc
     1801 gggccggtct cgacccgaca cctcgatcac cgaaaactca tcggacagac tattgttgat
     1861 gattgtctcc ggctcgacgg taaaggccgt gatgcgcggc tcggacggct tccgcttggc
     1921 catgagctca gccattcgca cctcgccctt gagcgcccga tcgatggtgc gtgtgatccg
     1981 cgccatgcgg cgcgcctcgt cggcgtcgtc gggcagctcc cgttgcagca ggaacgtgtc
     2041 gagggccagg ccgtcgcgcg tggtcgagat gtgtgcgccg atgatgttgg cgccgctggc
     2101 cgcgcaggcg ccagcaaaca gtgccagcag tcgtgggtgg ttcggtgcga cgatcgtcag
     2161 ctccgtgatc gcggtgaagg cgttggaggt gaaggaggtc acgagccgct gggcgtctgc
     2221 atccgcccgc cggatcaggt tcgcgtgctc gacctgcttg tgagtatcgg tcttgagcca
     2281 atagtcgtcg tagtggcgct ggatgaagcg ctcgacctct tccgggggcc agcccgccag
     2341 ctctgtccgc aattgcgcct gggcttccga gattcgttga cgccgtgaga cccgtgtatg
     2401 gccgccggcg acgaggggct ccgtctgcca atagagctcg cgcagaagct gacccttcca
     2461 gccgttccac gtgccggggc cgacggcgcg gatgtcggcg accgtcagca gaagcagcaa
     2521 tttcagccgc tccgggcttt gtacgaccgc cgcgaagtcg cggatcgtct tggggtcagc
     2581 aagatcgcgg ctgagcgaga tgttgctcat ttcgagatga tgctcgatga gccatgcgac
     2641 cgtgtccgtc tcggcagcgg acagcccgag ccgcgggcag agccgccggg caatgccggc
     2701 gccgaccttc gaatggtcct cgacccggcc cttgccgacg tcgtgcagga aggctgcgac
     2761 gtagagcgca cgccggttct gtatggagcc gatgatctcg gtcgacaggg gtaagctttt
     2821 cgcccgccgg ccttcctcga tgctgctgag ctcgccgacc gtgcgcagca ggtgctcgtc
     2881 gacggtgaag tgatgataca tgttgaactg catcatcgag acgacccgcc cgaactcggg
     2941 gatgaaccgg ccgagcacac ctgcctcgtt catacgcctg agggtcgtct ccggattgtt
     3001 cttggacgtg aggagctgca ggaaaattcg attggcttca ggattttgcc gcagctcgtc
     3061 gtcgatcagc ctgagggagc tgcgcagcac gcggatggca ttcgggtgga agaagctgcc
     3121 ggtttcctcc gccaccgcga aaaaccggat cagattgacc ggatcccgct tgaagacgtc
     3181 ctggtcggcg atattcagcc gctcgttgtc gatgcggaaa ccggtcgtcc ggcggatgcg
     3241 gcgccgcgtc acccaggtca ccgggttcag caactgctcc aaccgcgggg ccgacttcag
     3301 ctgctgcatc tcgagcgacg agcagaggat ggtcgtcagg tcgccgacat ccttcgccac
     3361 caggaagtag tgcttcatga atcgctcgac gccgcgcagc ccgcgggcgc tccggtagcc
     3421 cagccgctcc gccatgaccg gctgcagatc gaacgtcagg cgctcctctg cgcggcccgt
     3481 caggaagtgg aggttgcagc gcacgctcca caggaagctc tcgcaccggc gaaaggtcgc
     3541 catctcggca ggggtgaaga cgccggcgct ggcgaggcct ttgcccggcc cctcgccgtg
     3601 gacgtacatg gcgagccagt gcagcgtatg caggtcgcgc aagcccccct tgccgtcctt
     3661 gatgttgggc tccacacgat agcggctctc gccggaacgc cgatgccgct cgtcgcgctc
     3721 ggccatcttt gcctcgatga acgggcgggc aatggcgctc gtcaccacct cggcgcggaa
     3781 gcgggcttcg agctgtctga agagatcggc atccccgtga atcaggcgct tgtcgagcag
     3841 ggccgtcagg atggtgaagt cggacagcgc cagcttgatg cactggtcga tggtgcgcgt
     3901 cgcgtgcccc accttcaacc cgagatccca cagcagatag agcatgtact cggcgacgct
     3961 ctcgccccaa ggggtctgct tgtaggggag caggaagagc aggtcgatat cggagccggg
     4021 ggccaggagg ccgcggccga agcctcccgt ggcgacgatc gccatccgct cggcatcgga
     4081 gggattggtc gcgcagtaga cgtgcgtcac cgcgtagtcg taaatgagcc ggatcagctc
     4141 atcggtgaac tgcgagagcc cggcagcaca acgccggcca ttgccgtccg atgcaagctg
     4201 cgcctcggcg gaggcgcggg cgtgtttcag caaagtcctg caatagtcga ggactgcggg
     4261 ccgcgcgtcg ccggcgccgg ccttctcggt cgcaagccgc gccagagcat gccgcgcttc
     4321 gagcgggtcg aaatagggtg gcttggccag cgtcgtgacg tccatccgat aatccgcttc
     4381 agcaggtcac atacccgtgc cgatcccttt cccgttcaga agaggtcgaa gcgaggagcc
     4441 ggcgatcatc gccatcgccg tcgccatccc tcactcgtgc ccccgatctc tctcttcggg
     4501 agaggtggtg aaattgcctg ctgcgataca acctcaaacc agcggaaacc caatcctgcg
     4561 agccattcct agcgcgcctg cgactccttt cgccgcaggc tctttagctc atatagggct
     4621 tcgagcgccg cacgcggact gagctcgtct gggttgatct tatcaagagc cagctcgagt
     4681 tcggaggggg catcgcgtcc gcccgaggca ggtcgagagg gctcgtgggc aaagagcggc
     4741 aggtcttcca gcgagccgct ggtgccctgg cgacgatccg tcttttcgag ccgggccagc
     4801 acctcccgcg cccgcttgac gacggcaggc ggcaagccag ccagtttcgc gacctggata
     4861 ccgtaggacc ggtcagccgc cccgggttgc accttgtgca ggaaaacgat ctcgtcgttc
     4921 cactccttca cgtccatggt cacggcgacg agattgggca ggcggccggt gagcgccgtc
     4981 agctcatgat aatgcgtcgc aaacagcgcc cggcagcggc agacatcgtg cagatattcg
     5041 accgtcgccc acgcaatcga caggccgtcg aaggtcgcgg tgccgcgtcc gatctcgtcg
     5101 aggatgacga gggatcgctc gctcgcctgg ttgaggatgg ctgcagtctc caccatctcg
     5161 accatgaacg tcgagcggcc gcgcgccagg tcgtcggcgg ccccgacacg ggaaaagagc
     5221 cggtcgatga caccgatgcg ggccgagcgc gccggcacga acgagccgat ctgggcaagg
     5281 agggcgatca gggcgttctg ccggaggaag gtcgacttgc cggccatgtt ggggcccgtc
     5341 acgagccaga tgcggccgcc ctcgctgtcc tcgtcgaagc cttgctggcg cgctgcacct
     5401 tcccccagca cgcagtcatt ctcgatgaag ggtgacgcct tggcctggcg cagtgcctgc
     5461 tcgaccaccg gatggcgtcc gccgcggatc tcgaactcca gtccttcggt tacttcgggg
     5521 cggacgtagt tctcctcaaa ggcgagctcg gcgaggcctg cgcaatggtc gagctcggcg
     5581 agcgctgcgg cggtctcgca taacgcgcgc tcgagctctc caacggcccg cgcgagctcg
     5641 gcaaagacct cctgctcgat ggccagcgcg cgctcggcag ccgacgcgat cagcccctcc
     5701 gtttcggcca gctcgctggt ggtgaaacgg acggcattgg cgagggtctg gcggtggcgg
     5761 aattttgcgg agagaggctc tgccatcagc ggctttgcgt tggctgcgcc gacctcgacg
     5821 tagtagccga gcacattgtt gtgccggacc ttgagggtcc tgatgcccgt ctcctccaca
     5881 tagcgggctt ccagcgccgc catcaccttg cggctgtcgt cccggagccg ccgcgcttca
     5941 tcgagatcgg tgcggaaacc ttcgcggaca aatccgccgt cgcgccggtg caccggcggc
     6001 gtctcgacga gcgcggcgcg gaggccatct gccagcgcgc gggtttcggg ccgggacagg
     6061 cgcgtttcga tcgaggccag ctccacgggc aatccgatcc catcagccga gaggcggagc
     6121 agctcgccga gggcggacgc cactgtcagt ccgtcccgga cggcggcaag atccgcaggt
     6181 ccgccgcgct gcaaggcgag tcgggaaacg gcgcgcgcga tatcgggcgc gctgcgcagt
     6241 gcactccgga catcttcccg gaggcgatcg cgttcgagca gataggccag cgcatcaagc
     6301 cgcgtccgga tggtcctgac atcccgcagc gggcttgcca ggcgcgctgc cagctcgcgg
     6361 gcccccggac cggtgaccgt ccgatcgatg gcagccagaa ggctcccctg ccggccaccg
     6421 gacgctccgc gcacgagctc gaggctcgcc cgcgtggccg cgtcgatcac catcgcattg
     6481 gccggtcccg tgctgcgcgg tggacggatg atcggctggc ggccgatctg cgtcagctcg
     6541 acatacttga ggagagccgc tgccgcagcc agctccggcc gcgagaaggt gccgaatgct
     6601 gccaaatcgg ccacttcgag acgtgccttg atggcacgct cgccggtcat gctgtcgaat
     6661 gtggcaccag ggacgggcgt cgtcgcggct ccgatggcct cgatccaggt cttgatgcga
     6721 ggatcggcga aggtgccgtc ggttgcgatg atctcgctcg gctgcaaccg tgccagttcg
     6781 ccggacaggt cgctgattga gaccgagccg agctcgaact cgccggtaga gatatcgagc
     6841 gaggcgaggg caacgatctg atcaccgtcg tcggcgcccc gcggggcgac gtgcaaagag
     6901 gtgagatagt tgcgcgcttt cgcatccagc agcgaatctt cggtgatggt gccgggcgtg
     6961 acgaggcgga ccacgtcgcg tctgacgact gcctttgagc cgcgcttgcg cgcctccgcc
     7021 ggatcttcga gctgttcaca gacggcgacg cggtagccct gcttaatcag ccgctgcaga
     7081 tactcgtcgg cacgggctac gggcacgccg cacatgggga tgtcttcgcc gaggtgcttg
     7141 ccccgcttgg tcaacacgat cccgagcgcc tgggaggcaa caacggcgtc ctcgaagaag
     7201 agctcgtaga agtcgcccat gcggtaccag agcaggctgt ccggattggc cgccttgatc
     7261 tccaggtatt gcgccatgga cggtgttgcc tcggtgagcg cacgtcgcgc tgcgttggcg
     7321 ccctgaggcg catttgcgtc agcgtcatcc gaagctggcg aagtggtctc gtcgagctgc
     7381 agtcgctgcg ccatgagctg tcctgcccct tcattctccg gcaaccacat attcgtccct
     7441 gggctgcagg tcaaaagcgg ctccgctctt gcccactttt gccgtccagc aagttgccga
     7501 tgccgccatg aaatcgacgc gcacagctga ttgaacaatc gccggttgtg cgaactcgtg
     7561 cgcggaagtg gcccgcgcgc gccctagttc tcggaggttg tcatgcgttt tgccggggcc
     7621 ggcctgctcg ctcttctgtt gtttgtcgtg gtgccgccgg tgcagacggc tgcgcagcag
     7681 aaggcgaagg gcgcaggacc gttcgctacc aacgagcaga tcctgcagtg gatcaacgat
     7741 taccggtacg agccgaagcc gcagcgcctc ccggaggccg tcaaggcgat gagccgcctg
     7801 ggacttttca gggatgagga cacggcgggc gtctacgtcg ggttcatggc cggcgtgctg
     7861 gggagcaatc cgcggtcggc ggaaaagctt atcacccgca tgtacccgat gccccctgag
     7921 gaccagcacg cgatcatcaa ggccatcgcc tacagtggcc tgccggactg gaaggggctg
     7981 atgggcaagt tcgtcgagcg tatgccggcg cggaagacac tgatcgacag ctacctccag
     8041 gggaaggcgc ccgttctgca cgacttgccg ctcgacacgg ggccggcccc gatcgatacg
     8101 ctctggggct actacttcgc cacgggatcg ctcgagcccg tgtatcgcat cgtcggcgcc
     8161 ctgaggtggg cggacgacaa gagcgacctc aaccgtctga ccgccggcag cgttgccaaa
     8221 tggacattgg ccaacaacgc gacccgggac aaggagctgc tggatttcct gcgcgagcag
     8281 cagcacatgc gaaaccatcc cgagaaggtg cgaaaggtgc tcaaggaggt tgtggtcgcg
     8341 gcggaaacat tcgagaccgg caagatccgt aaggatgcgc tcgccgcgat cgaagaacta
     8401 aagcgcaccg gcccgcagcc gaacaactcc tgggccaaag ccgcctactg gggcagcacg
     8461 gccgtggctg tcggttgcgt cgtcgccagc gcactcggag cagcggctat cggcgtgccc
     8521 tgcatcctga ccggcgcggc gtcctcggcg gcggtgaagc tgctgaacgg gccgtgagac
     8581 acctgccggg aggctgaggc ttccggatgg cttggacttt ggcctcccgc taacccacct
     8641 gctccagagg ggagggcagg ttcacccggc cgggaactgc cgtgattggt cgagctcgcc
     8701 agggttctcc cgtcctacct cgagcagatc ggaaatggag agggcgcccc ccaaaggagg
     8761 cgcccagtgc atttcaaagg acagtctgcg ctgcggaaaa cgggccgggt gcaccggccc
     8821 gccgtgctcc cttcagttcg gcaccttctc ctgtgtggcg gccggggact ccgtcgaggc
     8881 cgcgaccttg ccgagcagga gggagatggc gtactggagc tgcttgtcct tctccggctc
     8941 ctgcggcacg taggccgacg agccagactc ttccttcacg ccctccttgt cgggcttcag
     9001 gtgaccgcgg aggctcgcct cgccacgggg cttggcaggc tccttgccct gcagctcagg
     9061 cggcagatcc tgctccacga cgatatccgg gtggatgcct tgcgcctgga tcgagcggcc
     9121 tgacggcgtg tagtagcggg ccgtcgtcag gcggatggcg ccgttgctgc cgagcggaat
     9181 gatcgtctgg accgagccct tgccgaacga ccgggtgccg atgaccgtcg cgcgcttgtg
     9241 atcctgaagg gcgcctgcaa cgatctcgga ggccgaggcc gagccgccgt tgaccagaac
     9301 gacgatcttc ttaccgtccg tgatgtcgcc cgggcgtgcc tggctgcgct gggtctcttc
     9361 cagattgcgc cccttcgtca gcacgatggc gcccttgtcg aggaacgcat cggaaaccgc
     9421 aacggcctgg tccagcaagc caccggggtt gttgcgcagg tcaatgatgt agcccttgag
     9481 cttcttgcca gcttccttct tcagctcatc caccgacttc acgaggttcg catgcgtctg
     9541 ctcgttgaag gtggagatct tcacgtagcc aacctgatcg tccgcctcga gccgcgcctt
     9601 cacggggttg atgcggatga tatcgcgcgt gatcgttacg tcgaacggct cgtcgcgacc
     9661 tttgcgcact atggtgagct ttatcggcgt gttgaccggg ccgcgcatct tttcgacagc
     9721 ctgctcgagc gtcagcccga cgatttgctc atcgtcgaga tgcgtgatca ggtcgttggc
     9781 ctggatgcct gcccgctgag ccggcgtgcc gtcgatgggt gcgaccacct tgaccacgcc
     9841 gttctccatc gtgacctcga tgccgaggcc gccgaactca ccccgcgtct gcacctgcat
     9901 gtcgcggaaa tgcttcgggc tgagataagc cgaatgcggg tcgagcgacg ccagcatgcc
     9961 gttgatcgcc gactcgatga gctgggtgtc gtccggcttc tcgacatagt cggaacgaac
    10021 gcgctcgagc acgtcgccga acaggtcgag ctgccggtaa atctcagaat tcgacgatga
    10081 cgccgaatag gtctgggtga cattcgctac tgtcgtcagg ccggcgaggc cggccatcag
    10141 caagaatgtc cagaatgcaa attcaggctt gcgcattgtc tctgtgcctt atccctgcac
    10201 cttcagctgc tctccctggt gccaccaggg agatgggtcg acggggcgac cttccttgcg
    10261 gaactcgaca tatagtaccg gggcattttc atcggactgg cccccggctg cgacctgcat
    10321 cgtcccgacc ggctcgccag caagtataaa ctggccgagc tgtgcgtcta tacgggaaag
    10381 tccggcaagt aatacatggt atccgcctcc cgcatttata atcaagagct ggccgtagct
    10441 acgaaactct ccagcgtaaa caacccagcc atcagaaggt gacgtgactt gggctccgta
    10501 acgggtctga atgactatgc cctgggactt gcggccattc gacgtcgttt ccccgtagga
    10561 gacgaccgtt cgcccatgcg cgggcatcgg caacttgccc ttggccaggt ggaagggcat
    10621 ggccggctgc atacgccccg gcggccccaa tagtggacct gatcccggtg acagcacgac
    10681 ggcgacctct gtctctttcg ccgttgcggc agccttgcca tccggcgcct tgccgtgcga
    10741 gccgggctgg cctgcatcgg cgcctttggg cagctgcttg ttgtaggtgg cgagcccggt
    10801 cttttcggcc accgtttgat cgagggccgt gatgagctcg ctgatgttgc tgaccgatct
    10861 ggagatccgg tcggcttccc gccgcacggc cgccagctcg ccttgccgct cggtcaagga
    10921 atggcgcttc tgctccatca gcatggccac gcgggcctgg gcagctttca aacgttcggt
    10981 ctccgcttcg atcttcgctc gttcttccgc gatctgtttg aaaacccggt cgagctcctg
    11041 caggcgcgcc acgaggtctt gaacttccgc ttgcagctcc ggaatggcgc gtccgagcag
    11101 catcgcactg cgcaccattt ccagcgcatc ctcccggcgg gtgacgatga ccggcggcgg
    11161 gttacggccg atccgctgca tcgccgcaag gaggctgctg atcgatgaat ggcgttgctg
    11221 cagggcctcc tgatgcatct tctgttgggc aacgagcgca tcgcggcgct cttctatgct
    11281 gctgagctgc gcctcgctga gctgaacgct gcgggcggcc gcctggagtt gctcgttgag
    11341 ccgggcgcgc tcctgctcca tggtcgcgag gtcggccttg agctgctttt cccgctccgc
    11401 agcctcctgg agccgcttgc gatcctgctc gagcttctgc ttggcctcgg tcgcgagctg
    11461 cgactgtgcc acgaccggcg aagcggccag cagaagggtg gccgccgccg cgaggcacac
    11521 cgtcgcacgc tgtccagccg cgcaccacct accgcccggg cgagccgctg atgccgcgtg
    11581 ttcccgtcgt gcccgcatct agcctcgttc cgcgaccctg cccaattgac cggcgcggtc
    11641 tcgtaccccg tcgcaacccc gcatcagaaa tctctgcaac cgaataaggc aattctggta
    11701 cggtgggcag tatcataata cgtgcaggcg gtgcgaccaa tcgcgaacct ttacttcact
    11761 ggtgtgtttc acgctatttg ggcgtcgtaa tgcgccgcca gcagctggcc caggagcacg
    11821 atccaaatga acagcctttt ctattttctc accatcctgg cgccggttgc cgttctggtc
    11881 gtcctgctgc tcgggcttgt caacctgatg cgagccagga acgcgaatac gagccagaag
    11941 ctcatgcggt ggcgggtcgg ccttcagttc gtggccatcg tcctcatcat gatcacagtc
    12001 tatctgaccc actgagctgg attcgctcca gcgcttcttg accggtcgcg cttcggagat
    12061 actgtcgtga gcagcgctcc cggcgagggg aaatcatggt cgttctgaac aagatctata
    12121 cgcgcaccgg tgatgacggc acgactgccc tcgcgagcgg ccaacgcgtg cgaaaggaca
    12181 gcatccgcgt tgccgcttat ggcacgatcg acgagaccaa cgcggcgatc gggatggccc
    12241 gcgtgcatct ggccggcagt catcctggcc tcgtcgaaaa gctcgcccga gtccaaaacg
    12301 atctcttcga ccttggtgca gagctcagca cgccggatac gggaaagcca aaagcgcgcg
    12361 aacggatgga gatcacgccg gcccaggtga agcgcctgga ggacgacatc gacgagctca
    12421 acgcccacct gcaacccttg cggtcattcg tgctccccgg cggctcgccc ggtgcggccg
    12481 ccctccatat ggcgcgaacg atctgccgcc gggcggaacg gttgatggtt acgatggcag
    12541 ccgaccccaa cgagggcgtg agccaggttg ctttacagta tgtcaaccgg ctttcggatc
    12601 tgctgttcgt cgccagccgc tacgtcaacg accgtgggaa gagcgatatc ctctgggtcc
    12661 ccgggcagaa caggtagaca agacagagcg tgagctgcca atctgcagcg gttcatctct
    12721 aggggtcggg cgcgtgttca ttccagtcta tgatcagaat ccgctgcgct cgatccagtt
    12781 tcaggccgtt acgaccggcc tgatcctggt gaacatcgtc gttttcatcc tccagataac
    12841 aacgctttcg aaccaggtga tcctgggctt cgcgctcgtg cccaacgacc ttctgggcct
    12901 cggcgcggcg gggcagggca ggttgctggc gccagggaat gagatcggca tccccgaacg
    12961 gctgacgctc atctcttaca tgttcttcca cggcgatatc ctgcatctcg ccggcaacat
    13021 gctgttcctc tgggttttcg gcgacaatgt cgaggatgcg ctcggccacc tcaaattctt
    13081 gatcttctat ctgacctgcg gggtcgtggg aggtcttgtc cacgcgctga tgatgccgtc
    13141 gtccgaaatg cccctgatcg gcgccagcgg ggcggtcgcc ggtgtgatcg cggcctacct
    13201 gatgctccat ccccgggttc gggtctgggt gctgatattt cggttcatcc cgatccagat
    13261 ttcggccgcg ttcgcgcttg gcgcctggat tttcacgcag ttcctgatgg tgctgctgcc
    13321 agaggtgggg cccgtcgcct ggtgggcgca catcggcggg atcattgccg gcgccgttct
    13381 cgtcaccgtg atgcgccggc ccggagtccc gctgttcgac cgcaatattg catagcagcg
    13441 gctacggatg cgaatagctg gcggaacacc ctgtcggccc gacgattgac ttgcagaaaa
    13501 cgtgagaata cggtgccctt tcgcacctgc gaagggtctc tcccacacgt agcccgcgct
    13561 gggtgagatc gaaggttgag acagatcgtc ccgcaatcga agcgttgcgg aagtcgcttg
    13621 gctgctggca aatacgttat gcgactgaag gaagattctg gagagtttca gatatgaaga
    13681 tcctcgtgcc ggtcaagcgc gtggtcgatt tcaacgtcaa gatccgggtc aaaccggatg
    13741 gctcgggagt cgagcttgcg aatgtgaaga tgtccatgaa ccccttcgac gagatcgccg
    13801 tggaagaggc gatccggctg aaggaaaagg gcatcgcaac ggagatcgtc gccgtctcca
    13861 ttgggccctc caaggcacag gagacgatcc gaaccgcgct cgcgatgggt gccgaccgcg
    13921 gtatcctgat cgagaccgac gagggcgtag ccgtagaacc tttggcagtg gcgaagcttc
    13981 tcaaggccgt ggttgccgac gagcaacccg gccttgttat tttgggcaag caggccatcg
    14041 acgacgattg caatcagacc ggccagatgc tggcggcgct ccttggctgg ccgcaggcga
    14101 ctttcgcatc caaggtcgtc atcgacaatg gctcggcctt ggtgacccgc gaggtcgacg
    14161 gcggcctcga aaccttgaag ctgcagctgc cggccatcgt cacgacggac ctccgcctca
    14221 atgagccgcg ttacgcctcg cttcccaaca tcatgaaggc gaagaagaag ccgctggacg
    14281 tcaagaaggc cgcggacttc ggcgtcgaca ttgcgccgcg cctcaaggtg ctgcaaacgg
    14341 ctgagccgcc tgcccgcaaa tcgggagtca aggtgtcgag cgtggcggag ctcgttgaga
    14401 aactgaagac ggaagcgggg gtgatctgat gccggccctg gttgtcgcgg agcacaacaa
    14461 cagcactttc aatccggtaa ccgcgaaagc ggtcacggct acggccgcgc tgggcagcga
    14521 gattcatgtg ttggtcgccg gctcgaactg ccggggtgtg gcggagcagg cggccaagct
    14581 tcagggcgtg acgaaggtgc tggtcgccga tgccccgcac ttggagcatc agctcgcgga
    14641 ggacatgacg gcgctcgtcg tcccgctgat ggccagctac gaggcgctcg tcgccgccgc
    14701 cacctcgttc ggcaagaacc tgctgccgcg cgtggcggcc atgctcgacg tcatgcagat
    14761 ctcggatatc acggccgtga aatcgcccga cactttcgag cgaccgatct atgccggcaa
    14821 tgccatccag acggtgcagt cgggcgaggc caagaaggtg atcacgatcc gtgcagcctc
    14881 gtttgcggca gctccggagg gcggatcagc gacgatcgag gccatttcag tgcctgaggc
    14941 cacgggactt tcgaagttcg agcgggccga gatcgccaag tcggatcgcc ccgagctcac
    15001 ggcagcccgg atcgtcatct ccggcgggcg cggaatgcag tccagcgaga acttcaagaa
    15061 atacctcgag ccggtggcgg atcggctcgg ggctgccatg ggggccagcc gcgcggcggt
    15121 cgacgccggc tacgcaccca atgacctgca agtcggccag accggcaagg tggtcgcgcc
    15181 ggagctttat atcgcggtcg gcatctcggg cgccatccag catcttgccg gtatgaagga
    15241 ctccaaagtc atcgtcgcga tcaacaaaga tggcgaggcg ccgatcttcc aggtcgcaga
    15301 ttacgggctc gtggcggacc tcttccaggc tctgccggag cttgacgccg agctgcagaa
    15361 agccggctac tgaaagcgaa aaaccggggc agcctgagaa gaacagatag aaacgccggt
    15421 ctgctgcata ttgtggaaca gtcaacgcca ggagcaaaca cggtggccat caagaaggtg
    15481 ggggtaatcg gcgccggcca gatgggcagt gggatcgccc acgtggttgc gcttgcaggg
    15541 tatgacgtag cgatcacgga catcgccaag gaggtcgtgg acaaggcgat gtccaacatc
    15601 gaccgtaaca tggaccggca agtcgcgcgc ggtcttatca ctgccgacca gaaagcggct
    15661 gccctcaagc gtatccgcta cgtgcccgac tacgacggct tgcaggattg cgacctggtc
    15721 atcgaggcag cgaccgagga cgaggcggtc aaacgcaaga ttttcaccga gctctgtccc
    15781 aagctgaagc cggatgccat cgtcgcctcg aatacatcct cgatttcgat tacccgcctc
    15841 gcctcgatca ccaagcgcgc cgagcgcttc atcggtatgc atttcatgaa tccggtgccg
    15901 gtgatgcagc tcgttgagct gatccgaggc atagccaccg aagacgacac ctttgcgacg
    15961 gcgcggtcct tcgtcgaatc cctggggaaa acgatcgcgg tctcggagga ttttccggcc
    16021 ttcatcgtca accgcattct gttgccgatg atcaacgagg ccgtttacac gctctacgag
    16081 ggcgtcggaa cggtggaagc catcgacaag gccatgcgac tcggcgcgaa ccacccgatg
    16141 gggccgctgg agcttgctga cttcatcggt ctcgacacct gcctttccgt catgcaggtg
    16201 ctctacgaag gcttggccga ttcgaaatac cgcccctgcc cgctattggt gaagtacgtc
    16261 gaggccgggt ggctcggccg caagacccag cggggcttct acgattaccg gggcgaaaag
    16321 cccgttccga cgcgctgata tcgagcccgt caggaaagct gggcggattt gcacaccttt
    16381 cgttcccagc cgcactcgac gccggcattg accgcaatat ttcctattca agcgcggctt
    16441 gagctatatc cctttttgat tccttgagtg cggctagcct tgtgactgcc tgctccgatc
    16501 atctgcgctg cgttatgaag ccaggcagca tgggaacggc caaagaggcc ggaacgggcg
    16561 aagcgaaggt gcgcgaatga ctggcgatct cgacaaggag taccggccct gcgtcggcat
    16621 catggtggtc aaccgacaag ggttggtgtg gatcggtcgc cgcgccgatg cgccgaatga
    16681 gccggaaggc cccggttcct ggtggcagat gccgcaaggc ggcatcgatc ccgacgagga
    16741 cccccgcatc gcagcgctcc gcgagttgcg cgaggagacc gggttgcaca cggtcaaggt
    16801 tatcgccgag cacccggaat ggctgcgcta cgatctcccg ccaaacctga tcggcaaggc
    16861 ctggggcggg cgctggcgcg gccagcggca gaaatggttt ctcgtcaggt ttctcggcga
    16921 cgactcggaa gtgaatctca ccccgcctcc tggccaccag gtcgagttcg actgctggcg
    16981 ctggtccccg ctcgacgagc tgcaggacgc gatcgttcca ttcaaacgtg atgtctatgc
    17041 caaggtgatt accgcttttc gtcccctgat cggacccgat accgggagcc gttgccagtc
    17101 gtgactttca aaaccggcgc cggtcgacag aaattgcccc tccggcgcac cgtcggcatc
    17161 ttactcatca atcggagtgg aaaagtgtgg atcggccagc ggcggccgag atggcttccg
    17221 cccgatgccg aaccgatctg gcagatgccg cagggtggca ttcgcgcagg cgagaaaccg
    17281 cgggcggccg cgctgcgtga gcttgccgag gaaaccggaa tcacctcggt cgaggtgctg
    17341 gcaaaatcac ggtcctggat cacttggcag cttccggacg aactcatcgg cgtcgcgctt
    17401 aaggggcgtt acaggggcca gcgccagcgc tggttcgcga tgcggttcgt cggtgatgat
    17461 cgtgaggtct cgctgacccc accggacggg acgaaacccg aattcgacga ctggcgctgg
    17521 gccaagcccg aagaggtcat cgcgctggcc attcctttcc gcaggcccgt ttatgcggcg
    17581 gtcatcaagg agtttgcttc tttactccaa aaagaatagc ggacccgttc gggtccgccg
    17641 cgtctgctta aattcggtgc ttggatcagc ggccaccgga ctgcagggcg cggagccggt
    17701 ctacggcttc ctgagccatg aaccgggcgt cgtcgtccgc ggcctcgtcc agctccgcct
    17761 gagcagccgt cagctccgcc tcgattgcgc tctgcgcgga ggaggcatct gcatcgaacg
    17821 ccgtctgcgc gagcaccgtc agccgatccg gctcgacctc cgcgaagcct cccttcacga
    17881 atacgcgccg cgttccgctt gggagctgca cgtcgagcac gccagggcgc agcatcgaaa
    17941 gcatgggcgc atggttcgcc agaactgcga agtcgccctg aacgccgggc agcaccacct
    18001 gctccgcatc ctctgacagc agcacccttt cggggctcac cagctcgaat ttgaacgtat
    18061 cagccattct ctttcctcag cgccggcccg agggcagccg tgccccgtgc gggcatggct
    18121 actcgtcccc cgggtcctcc tcggccttcg cctgcacctc ttcttccgtc tgcagctgcg
    18181 tgccgcagaa ggggcagaag tacaccggat gatccaccgt gccgggctcg ttgtcgtcga
    18241 gctcgatgag cccgaccgcc aggtaaagca cgccgtcgcg gcctacactg acgagcggct
    18301 cgaagtcctc gccggccatc gcttccttca gcgcgtcaca gcacgtgccg aacccgccac
    18361 tcgccgtctt ttccgcttca gccatatgcg ttcactttct tgaccgctca ggcggcctct
    18421 gcgagctgct ctgccttggc gatggcttcc tcgatggtgc caaccatgta gaacgcctgc
    18481 tcgggcagat ggtcgtagtc gccgttgcac agacccttga agcccttgat ggtgtcctgc
    18541 aggctcacga gcttgcccgg cgagccggtg aagacctccg cgacgtggaa cggctgcgag
    18601 aggaagcgct cgatcttgcg cgcacgcgcg acgatcatct tgtcctcttc gctgagctcg
    18661 tccatgccga gaatggcgat gatgtcctgg agcgacttgt agcgctgcag gatcgcctgg
    18721 acctggcgcg cgaccgcgta gtgctcctcg ccgacgatgc gcggatcgag catgcgcgag
    18781 gtcgagtcga gcgggtcgac ggccgggtag atgccctttt cggcgatggc gcgcgaaagc
    18841 accgtcgtgg cgtcgaggtg ggcgaacgag gtcgccggcg ctgggtcggt cagatcgtca
    18901 gccggcacgt aaatggcctg gaccgaggtg atcgacccct tctgcgtcgt cgtgatgcgc
    18961 tcctgcaggg cccccatgtc ggtggcgagc gtcggctgat aaccgacggc cgagggaatg
    19021 cggcccagaa gcgccgacac ctcggagccg gcctgcgtga agcggaagat gttgtcgacg
    19081 aagaacagca cgtcctggcc ctggtcgcgg aagtactcgg cgaccgtcag gccggtcagg
    19141 ccgacgcggg cacgggcacc cggcggctcg ttcatctgac cgtagaccag ggcgcacttg
    19201 gagccggccg cagagccgtg cttgcgcggg tccttgttga cgcccgactc gatcatctcg
    19261 tgatagaggt cgttgccttc gcgcgtgcgc tcaccgacac cggcgaacac ggaatagccg
    19321 ccgtgcgcct tggcgacgtt gttgatgagc tccatgatga gcacggtctt gccgacgccg
    19381 gcgccgccga acaggccgat cttgccgccc ttggcgtagg gcgccagcag gtcgacgacc
    19441 ttgatgccgg tgaccaggat ctgcgcttcc gtcgactgct cgacgtactc gggtgccggc
    19501 tggtggatgg ggcgctgttc gacgccctgg atggggccgg cctcgtccac cggctcgccg
    19561 atcacgttca tgatgcggcc gagcgtggcc tcgccgaccg gaacggcgat ggcgcgaccg
    19621 gtatcggtga cctcctgacc gcgcacgagc ccttcggtcg agtccatagc aatggtgcga
    19681 accgtattct caccaaggtg ctgggccact tccaacacca ggcggttgcc gtggttcgtc
    19741 gtctcgaggg cgttcaaaat tgccggcagc tcgtcctcga actgaacgtc gacgaccgcg
    19801 cccatcacct gacgaattct tccgacttta ttgcttgcca ttgtgtcttc ctaacctgca
    19861 gcggttttca gtctgcttcg acctaatcga gacgtcgctc tatcctcgat attctttcct
    19921 cgagccgatc caggcgctgg ctcagacttc cgagcagagt caggacctga ccctggttca
    19981 gctccaagcc cgaaaccctc gatttcaggt cgcctacgtc caactccatt cgctcctgag
    20041 acgcgcgaat gcgtttcaag tgctcgagga tcaaattcga actttcgtca gtcatggcga
    20101 actctgccga cgttgctgac cacccgccga cgctatagcg cctccgcacc cgagatgatc
    20161 tcgatcagct ccttggtgat catcgcctga cgtgtgcggt tataggtcaa tgtcaacctg
    20221 tcgatcatct cgccggcgtt acgcgtcgcg ttgtccatcg ccgtcatgcg cgcgccctgc
    20281 tcggaagctg cgttctcgag aagaccgtgg aagacctggg tcgacaggtt ccgcatcagg
    20341 aggaactcga ggatctcctc ctcgcccggc tcgtactcct ggatcgcctg ggcgccggcc
    20401 tgggcattcg cttcttctcc ggccggcagc ttcgccggta tcagctgctg tgccgtcggc
    20461 ttctgtgcga tgaccgactt gaactcggca aaatagagcg tgcagacgtc gaactcgcct
    20521 gcttcgaaaa gggcgaggat cttgtctgcg atctcctgcg cgtttgcata accgagttgc
    20581 cgtacgccgc gaagatcgac ccggtcgacg accagcttac cgtattcgcg acgtaggatg
    20641 tcgtaacctt tgcgaccgac gctcaagatc ttgaccgtct tgccctcctg ctgcaggcgc
    20701 tggatgtcct gccgcgccag cctggcgata ttggcattaa aaccgccgca aaggccgcgc
    20761 tccgctgtca tgacgacgag cagatgcacg tcgtcccggc cggtgcccac gagcagcggt
    20821 gacaggccct cccgcgaggt aatccgctgg ttgaggttaa ccagaatgcg gtccatccgc
    20881 tcggcgtacg ggcgcgccgc cattgccgct tcctgcgcgc ggcgcagctt ggcggccgca
    20941 accatctgca tggccttggt gatcttgcgc gtcgccttga cggaagcgat gcgatttctg
    21001 aggtccttca agctggccat agtgccgcgt cgtccccgtt gcgatcccgt cgacccggac
    21061 ggcacaggca catgaaatgc cgcgggccgc gatccgggtg aaacttgccg aaggcgctgt
    21121 ggtcctgatc tccagcctgc aaggtacctt cggcgctcct tgcgctagaa cccggcccgc
    21181 gttggacttt cgcccgcggg cgagccggga gcttcaatat cgacttaggg cgtgaacgcc
    21241 ttcgcgaact tgtcgatagc ggcgaccagc tgcttctcgg tctcggccga aagctgcttc
    21301 tcagtccgga tggtctcgag aatatcggcg tgctgatcgc ggatcgtgcg cagcagctcc
    21361 tgctcgaagc ggccgacggc ggtcaccggc agcggatcga gatagccgcg cgttccggcg
    21421 aagatcacga tcacctgctc ttccatcttc agcggcgaga actgcggctg cttcagcagc
    21481 tcggtcaaac gcgcgccgcg cgccagcagg cgctgggtgg tggcgtcgag gtcggagccg
    21541 aactgggcga atgccgccat ctcgcggtac tgcgcgagct cacccttgat gcggccggcg
    21601 acctgcttca tcgccttcgt ctgcgccgcc gacccgaccc gcgacaccga gaggccgacg
    21661 ttcaccgcgg ggcggatgcc ctggtagaac aggtccgtct cgaggaagat ctggccgtcg
    21721 gtgatcgaga tcacgttcgt cggaatatag gccgacacgt cgttggcctg cgtctcgatg
    21781 accggcagtg cggtcagcga gcccgcgccg tgctcgtcgt tgagcttcgc tgcgcgctcc
    21841 aaaaggcgcg agtgcaggta gaatacgtca cccgggtagg cctcacgccc cggcggacgg
    21901 cgcagcagaa gcgacatctg gcggtaggcg acggcctgct tggacaggtc gtcgtaggcg
    21961 atcacggcat gcaggccgtt gtcgcggaaa tattcgccca tcgtgcagcc ggcgaacggt
    22021 gcgagatact gcatcggcgc cgggtcggag gccgtggccg caacgatgat cgagtactcg
    22081 agagcacccc gctcctccag caccttcacg aactgagcga cggtggagcg cttctgtccg
    22141 acggcgacgt agacgcagta gagcttggcc ttctcgtcgt caccctggtt cagcggcttc
    22201 tggttgagga aggtgtcgag gataatggcg gtcttgccgg tctggcggtc gccgatgatc
    22261 agctcgcgct ggccacggcc gatgggaatc agcgcgtcga tggccttgag gccggtcgcc
    22321 atcggctcgt gcaccgattt gcgcggcagg atgcccggcg ccttcacgtc cacgcgcatg
    22381 cgtttcgtgg ccttgatcgg ccctttgccg tcgatcggat tgccgagcgc gtcgacgacg
    22441 cggccgagca gctcaggccc caccggcacc tcgacgatgg cgcccgtccg cttgacggta
    22501 tcgccttcct tgatgccgct gtccggcccg aaaatgacga cgccgacgtt gtccgcctcg
    22561 aggttgagcg ccatcccgcg gatcccgttc gggaactcga ccatctcgcc ggcctgcacg
    22621 ttgtccaggc cgtaaacacg ggcgatgccg tcgccgaccg acagcacctg gccgatctcg
    22681 gagacctcgg cctccttgcc gaagttcttg atctgctcct tgaggattgc agagatctca
    22741 gcggcgcgga tgtccatcag ccaacctctt tcatgcgaat cttgagtgaa ttgagtttcg
    22801 tgcgcagcga tgagtcgatc atccggctgc cgatcttcac gacgagaccg ccaaggaggg
    22861 cgggatcgac acgaaggtta atttgcacgt ccttgccgac ggacgccctc agcgcgtcct
    22921 tcagcgcctt catctgatcg tccttcagcg ggaaggccga agcgacatcg gcctcgacct
    22981 cgccacgggc cttggcgcac atggtgcgga aagcccggat catatccgaa accgcgaaca
    23041 gccggcggtt gcgggccgcc agcttcagga aattggctgc cagcccgccg atgccgaccc
    23101 gctccagcac cgcagatatg gcggcaagct gttcgtcgct ggaaaagacc gggctgcgca
    23161 ccaggcgcct caggtcctcg ctgtcatcga gcagcttctg gaactctatg aggtcctgct
    23221 ctacctccgc gagctgtcgc tgctcctcag ctagctcgaa tagtgccgaa gcgtagcgcc
    23281 ctgctacact tgccataatc ggttcgtcgg tcgccacgtc gaacggcctc gtcgcgttga
    23341 ggatattggg ggcattagcc gcggcggtca cccgctcggg cgaaggagca tctgcaaaag
    23401 ggcacccgcg cggcttggca ccccttccag aagccgggtt ccctctaaca cggtaggcgc
    23461 agcccatcaa ccgccggaat acgcctcaat gccgcattcc ggtctcaatc aattccggcg
    23521 cggaaagctc tcggccagtg ccgatttcct cgtgcgggca aatgcctcca atcaggttcg
    23581 aagaatggct acgagctaca tgaaattgta gggatcgacg tcgacggtca agcggaggtc
    23641 gcccttggga tcgggtgcgg ccgacagcca ggcgcgcagg taggcctgca ggtccacctc
    23701 gcgggccgcc ttgacgagaa tgcgccagcg atggcggccg cggatcaccg ctaagggcgc
    23761 ttctgccggc cccagcacct caattctctc ggcgagtggc gcgcggcgca ccacttcgcg
    23821 ggcgtagacc tcggcagtct ccttgtgacg ggcagatata atgagcgccg ccagccggcc
    23881 gaacgggggc agcatggcag cttggcgctg gctgatctcg cggtcgagaa aggcctcgcg
    23941 gtctccggag atgatcgcct gcatgacggg atggtcgggc atgtgcgtct gcaccaggcc
    24001 gcggccctgg gcaaaggagc ggccggcacg gcccgtcacc tgatgcagca gctggaacgt
    24061 gcgttcggcc gcccgcgggt ccgcttggcc gaggccgagg tcgccgtcga cgacgccgac
    24121 cgtggcaagg tcggggaagt ggtgcccctt ggcgacgatc tgggtgccga tgacgatatc
    24181 ggcctcgcgg ttctcgatgg ccgcgatcac ctttcgcatc tcggcgaggc cggggatcag
    24241 gtcgctcgac aggattgcca gccgggccgt aggaaaccgc tccgacactt cttccgcgat
    24301 gcgctccacg ccaggaccgc aggccaccag cgactccgag tcaccgcact tcgggcactt
    24361 ccccggaatg ggcagtgcga agccgcagtg atggcaggtg agcttgcgcc ggaaccggtg
    24421 ctccacgagc caagcggaac attgcggaca ctcgatcctg gtgccgcagg tccggcagag
    24481 cgtcagcggc gcgtagccgc ggcggttcag aaagagcaga gtctgctggc ccttggccgc
    24541 cgtctctgcg atcgcttcga ccaggaccgg ggacagccag cggcccttct cgggcggatg
    24601 cttgcgcaga tcgatggcct cgacctgcgg cagctccgta ccggaatagc ggcccggcag
    24661 gacgagatgg cggtagcggc ccgtgcgggc attcacgtag gtctcgatcg acgccgttgc
    24721 cgaggcgagc acgaccgggc atttgccgag gctgccgcgc acgaccgcca tgtcgcgggc
    24781 ctgatagtgg acccggtcgt cctgcttgta gccctgatcg tgctcctcat cgacgatgat
    24841 gaggccaagc tcgcgatagg gcaggaagag tgccgaccgc gcgcccacga cgacgcgggc
    24901 ctcgcccgtt gccgtcgctc gccacacccg cccgcgctcg gccgaggcga gcgccgaatg
    24961 ccactcgacc ggcgggcagc cgaagcggct cgcgaagcgg ctgagaaact ggctcgtgag
    25021 cgcaatttcc ggcagcatga tcaacacttg ccggttccgc tccagggccc tggcgacggc
    25081 ctcgtagtag acttcggttt taccggagcc cgtcacaccg tcgagcagcg tcactgaaaa
    25141 gttgtccgcg tcgacggctg atcgcagcgc gtgaacggcg cgctcctgat ccgggccgaa
    25201 ctggaccatg ctgtgggccg gccacggctg cggataacgc ttttctggga tcgcaacctc
    25261 gaccagaacg cccgcctgga cgaggccatc gataacacct gtggtgcagc ctgcctccgc
    25321 ggccaacgcg gatttggcgc gcgccagacc gtcgcttgca atctcgatgg cgcgtttgcg
    25381 ggcaggggtc atgcggggcg gctcgggcgc gccctcgacg atgcgcacgc cgaagcgggg
    25441 ccgcggcggc tcgaaaaccg cctgcgcgcc catcatcatc cgcaccacca tgccaagcgg
    25501 cgagagcgtg tagcgcgcga tccattcggc aaagcgcagc gacagggtgg ggagaggtgg
    25561 gacgtcgagc acctccagca gcggcttcag cttggaggcg gtgaccggcc gctcgggacc
    25621 gacaacgcga tcccagacga cgccgagccg gagctgcggt ccaaacggca ccaatacgaa
    25681 cgagcccggc tggatatcca acttttccgg tgccaggtaa tcgtaagatt ggtccagagc
    25741 taccggaatg acgacaggca ggcgacgcaa agcgccgcaa gcttcgtcat taagttccag
    25801 cagactggtc aaaggggcac gctgcagatt agaaccgacc caaagcggct agccttggcc
    25861 gagccttgcg gcgagccaag ggcaacagga gttgatccat gaaattcttc gtcgacacag
    25921 cagatgttgc cgtcatcaag gagctcgcgg cgaccgggct gctcgacggc gttaccacca
    25981 atccatcgct ggtggcaaag gtggggcggg acttcaagtc ggtggtgcgg gaaatctgtg
    26041 agatcgtacc ggggcccgtc agcgccgagg tggctgcgac cgactatgag ggaatggtcg
    26101 ccgaaggtcg tgtgctcgcg gcaatcgcca agaatgtttg catcaaggtg ccgttgacct
    26161 gggacggcct caaggcctgc cggacactca cttcggacgg gcacatggtg aacgtcacgc
    26221 tgtgcttctc ggcgaaccag gcgctgctgg cggcgaaggc cggggctacg ttcgtctcgc
    26281 ccttcatcgg ccgtctcgac gacatcggtc tcgacggcat ggagctgatc cgtgagatcc
    26341 gcgccatctt cgacaactac tcggagctga cgacggacat tctggccgcc tcgatccgca
    26401 ccgtgaacca cgtccggcag gcagctctca ttggtgcaga cgtcgcgacc attccgcccg
    26461 ccgtcctgca agccctggtg aagcatccgc tgaccgacat cggccttgcc cagttcgtgg
    26521 ccgattggga aaagacgggg cagaagattc tctagcgaat agtgggtggc gaatagcgag
    26581 tagggatgac gtaggagagg gccggaactc tccccaccgc catccccgcg caggcaggga
    26641 tctgcgatac agcgccgcga gcactcgttg caaggtgtcg tgatggccac ctccgcggcc
    26701 atgacggatc gaaggatggc gtgccttgga cctttagccg tcccgcaacg cggacctgac
    26761 ggtgggagat gtggccggat cagcccagct gctcccttcg ccattcgcta tttcgccatt
    26821 cgctcactct gccgccgtcg gcggctcgag caaatcccag cggttgtgca gcatggtcag
    26881 cgcgcggcgg gcggcttcgg cctgggcagt ggcaccatgt tgctcaaaga cctccagcgc
    26941 cgggacgatg gccgccgcgg cagcgagcca gttttcgcgc cggtccttca gctcgccgag
    27001 cctgatgagg acggtcgaca tgttgagccg cgtgaccgcc cagcgcatcg gctcggattc
    27061 acgcgagaac acctcgagtg cgccccggta ggcgaccgcc gcctgctcca gcgtcagcgc
    27121 cgaggcattc ccgacctcgc acatggacgc cagcgcgttg ccaaggccga tccgcgccag
    27181 accccagatg cgggggtcgg tttcgcggct cagcacctcg agcgccgtcc ggaaggcgat
    27241 gctcgcctgc tcgaaaaggg ctttggacgg ctcgtgctcg gcgatggcaa gcagcgcatt
    27301 gccgaggccc tgtgaggccg atgcgaggcc cttcgcctct ccattggagc ccgccagctc
    27361 ggcggccttg gcatagacat tcacggcctg ccgcaggagc gggatgttgc tggtctcctc
    27421 gcctgcgtag tagagcgcat ccgcgcggcg caacgtaaag cgcaactggg cagcgggatc
    27481 tgtgcgagcg acactctcgg ccgcctcctc gaacagctcg gcggcgctct ggaagtccat
    27541 gcgcgccatg gcgagctcgg cgacgtcggc ggttgccatc gcttcctgct gaatctcggc
    27601 gcgaagctgc tccagctcct cattgagcct ctgctcggtc cgccggcgcg ctgcgcgaaa
    27661 actgcggcgc acctccttca gcagctccat cgcggcttcg aagtcgccgt tgaccagcgc
    27721 ttcggctgcc ttcgccttca gtgcccggat ttcagcgggc tcgttcgtgg gccgtaggag
    27781 ctgctcccgc gtctccttca gccactgcgc cagctcggcg acctgccatg cctcgcgggc
    27841 gggcagggtg cgctgctttt cggcaatggc cgaaaaccgc tgcaaggccc tgtccgacaa
    27901 gccgtagcgc tctttgagag cagccatctc ggattcgggg cttgcttctg cgacggtgag
    27961 ctctgtgcca ggttccgatg ctgccgccgg ctcgggctgc tgcttgccca gaaactcgct
    28021 tgctgccggg agcgcatccc ggaggagcct ttggtcggcg agaacttgca gaaacagctg
    28081 ctcgagcagg aagttgcaga cgtcgtcaga aagcccgtca aagccctccg ggccaagggc
    28141 cttggcttgc gcgacgagct gcgccgcgat ctggctcgct gcagcggccg gggttccgtg
    28201 ctgcccgcgg agcgcagcgt tcacgaattc cgggtccgga cggcacgcgt cgatgacggg
    28261 caccagcctg cgggcatgtt ccgccgccgt gtgcatcggg atgccgcgcg tatcttgggc
    28321 gagccagatc tggtgagcga ggcggtcgag cacatcgccg atgggcccac cctcgccgcg
    28381 cgatcgtagg aaggccgaga actcgccgcc agagagggca gccccaggcg gaacgcggca
    28441 gccgagagca ggcatgaggg cgagcacccc tgctccggtg aaaagctcca atgactcata
    28501 aagggacatg cctgcacgct cgccgctcgc gcatcccgac aagtaccgca tcgggaaagc
    28561 gaagtcatgc caacgcgtaa cgatctgtgc acagctgtgc gaatctgcgc agtatggaag
    28621 gtcgaaacgt tgcactcgga gcattgttaa cggtccgtta accctggaga cctgaagtcg
    28681 ccggtcatgt cgtaagctaa cggagcggtg tcgtatgcga gccatggccg aagggcaggg
    28741 ggagttgcaa ctctccagac cgctgcagga ggcggtcgcg gactggctcg accacctcgc
    28801 tggcgaacgc aactattcgg accttacgcg tgaggcttac gggcgcgacc tgaggcagtt
    28861 cctcagctac tggcaggcac agctcggtcg tcagccgagc ctcgccgatc tcgagattct
    28921 ggagccgcgg accttccgtg gctttctcgc aagccgccgc cgccagcagg catcgaatcg
    28981 ctccctggcc cgatcgctct cggcactgag gtcttttttc cgctggctgg aggggaagga
    29041 aatcggacga agccgggccg tgctctccgt ctcacgtcca aaggtgccgc acggcgtgcc
    29101 gaagccgctc accgtggcca aggccgctgc cctcattgaa agcgggatcc aggcggaccc
    29161 tgactgggtc gtaacgcggg acacggccgt gctgctcctg ctctacggat cggggctgcg
    29221 catctccgag gcgctgggac tgaaagtctg cgaagctccg agcaaggatc gcgacgtgct
    29281 ccgcatcgtc ggtaaaggcg ggaaggagcg ggtggtgccg gtgctaccca tcacgatcga
    29341 ggccgtggag cggtatcgcc agcaatgccc ctaccttctc gagccggatg atattttgtt
    29401 tcgcggagcc aagggcggtc cgttatcgcc gcggatcatt cagctcgcga tcgcacgggc
    29461 gcgtgccgca ctgggattgc ccgagacagc gaccccgcat gcgttgcgcc actcgttcgc
    29521 aacgcatctc ctctcggccg gagcggactt gcgacagatc caggagctgc tgggccacgc
    29581 atcgctgtcg accacgcaaa tctacaccga ggtcgaccgc gcgcggctgc tcgccgttta
    29641 tgatgccgcc caccccaggg cgcgcggctg atccgtcgca atcacatgtg gatggcgcgc
    29701 ttgtcgacgg cgagcgcggc ttccttcacc gcttctgaca aagtcgggtg tgcatggcag
    29761 gtgcgggcca ggtcttccgc cgagccgccg aactccatga tgacgacggc ctccgcaatc
    29821 agctcgcccg cattcgggcc gatgatgtgc acgccgagga tgcggtcggt ctcggcgtcg
    29881 gccagcacct tcacgagtcc gtcggtggtc ttgttcacct tcgcgcggcc gttggccgtg
    29941 taggggaact tgccgacctt gaacgagacg cccgcctcct tcagctcctc ctcggaacgg
    30001 ccgaccgtgg cgacctccgg aaatgtgtag atgacgctcg gaatgacccc gtagttcaca
    30061 tggcccgcct gtcccgcgag tatttcggcg acggcgacgc cctcctcctc ggccttgtgc
    30121 gccagcatcg gccctgcgat cacgtcgcca atggcgtaaa tgccggggac gttggtctgg
    30181 taatggtcgt caacaacgat gcgtcgcttg ttgtcgagct gaacgccgac gtcctccagt
    30241 ccgagtccct cggtgtatgg cacgcgcccg acacagacga gaacgacgtc ggccgagacc
    30301 gtttcggcac ttccgccggc agcgggctcg atggtcacct tgtgcgcgcc gttcgacttg
    30361 ttgacggcgg tcaccttgct cgacagcttg aatttgaagc cctgcttcgt gaggatgcgc
    30421 tggtaattgc gggccatttc ggcgtcgaga ccgggtacga tgcggtcgag atactcgacg
    30481 acgagcacct cgctgccgag ccgccgccac accgagccaa gctccaaccc gatcacgccg
    30541 gcgccgatga caagcagttt cttcgggact ttcggcaggg aaagcgctcc ggtcgaggag
    30601 acgaccgtct tctcgtcgat gtcgatgccg ggcagccggg cgacgtcgga acccgtcgca
    30661 atgacgatcg ccttggcttc cagctgcttc ttggcgccat cgtccgcggt cacctcgacc
    30721 ttgcccggcg cggtgatgcg tccggtgccg tggaacggct cgatcttgtt cttcttcagc
    30781 aggaatgcga cgccgtcgac gttgcccttg acgccctcat ccttgaaggc gtgcatgcgg
    30841 gcgaggtcca gctccggctt gacgccgatg cccatggccg cgaaggcgtg attggcttcc
    30901 tcgtaggcct cggacgcgtg caacagcgct ttcgacggca tgcagccgat attgaggcac
    30961 gttccgccaa aagtgggtcg cttttcgacc acagccacct tcatgccgag ctgcgccgcg
    31021 cgaatggcgc acacgtaccc cccggggccg gtgccgatga cgataagatc gtaggtcatt
    31081 tctgtttctg ctctgtactt gttcagcttg cgaggcggag gatttcggcc tgtcgaatag
    31141 cgcgacggcg cagcgaatcg cttccgaaat caacttcgtc gccatcatca tccagccaga
    31201 agatcgaatg cccgtactct tccaacttca catcgttgaa acgctccggc gatttccgta
    31261 gaaactcgaa gacctctccg tccgctatga cggggcgcaa atccacgatc gcctcgaaac
    31321 catccagcca cgtaatcttg aggactccgt acaggaccgg ctcgacggcc ttcattcgaa
    31381 tgatctcgtt cattcgattt tcgcaggctt ccggcctgcc tccatctcga accaggcgtc
    31441 aatcagctcg tctcgatgtg ctctggccca atccaacacg tcgctttcct tattcggagg
    31501 caaagatcct ttcattatct gtacgggatc gatcccaatt tgagcagtga actcagcaat
    31561 tctcgcgtgg aaatgagggg gcggatgctc atcagcatag aatagaatct ttacgccact
    31621 gacgcttgcg accgttggca tcgccagtgc cgcctcaaag ctccagcacc agccgctgcg
    31681 ggtcctccag aagctccttc acgcgcacca ggaacgtaac ggcttccttt ccgtcgacga
    31741 tccgatgatc gtagctgagc gccagataca tcatcggtcg tacgacgatc tgaccatcgc
    31801 ggacgaccgg gcgctcctcg atccggtgca tgcccaaaat tccggattgc ggtgcattga
    31861 ggatcggcgt cgacaggagt gag
//
LOCUS       DASVDU010000171         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1010331, whole genome shotgun sequence.
ACCESSION   DASVDU010000171 DASVDU010000000
VERSION     DASVDU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010331"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..1871)
                     /locus_tag="VF226_10860"
     CDS             complement(366..1871)
                     /locus_tag="VF226_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7074530.1"
                     /translation="MNRRTLIQGALASAAALSAPGAFAALDAGKRSAIKRAVEAGFEA
                     SLRRIQDWIRLPTVAAEGLNIEEGADYMARLAKEAGFSRVRKVPTGGSPAVFGVLDVG
                     ARRTVGLYFMYDVKQFDEPEWSSPPLEPTMFDHPLGRALRGRGVINHKGPEATFLAAI
                     HAFHSAGAQLPVNLVLLAEGEEEIGSPNFTNVFKDEEVLREFQRADAVFMPTSLQGLG
                     GEIPICLGAKGIIECELIASAERWGRGATQDVHSGFKASVDSPVWRLIDALSTLVRDG
                     GNTPAIEGWLEHYRPLTERERELIAQAARNSNEADLKRAYGIKRWINDMSWREALEAN
                     AARPTVNIEGLVAGYTGAGGKTILPGRATAKLDMRLVPDMTAKDCLSKLRAHLDKRGF
                     TDIEIKVGGAYDPTQTAEDSALIRAEKAVLARLGVPHSIVPRIAGSYPGYMFTGAPLG
                     KPFNQFGLGHGGRAHAPDEFYLIESNDPKVAGLREATMGYVEFLYELASTS"
     gene            complement(1868..>2858)
                     /locus_tag="VF226_10865"
     CDS             complement(1868..>2858)
                     /locus_tag="VF226_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7074531.1"
                     /translation="PVTEEIDVPFKSTVTAEYLGKKVGVMHACGHDAHTANLMGVAEA
                     LTSLRRDLPGNVLFVFQPAEEGPPEGEEGGAELMMRQGIFRDPAPEAVFGLHVFAGMP
                     AGTIGYRSGPFMAAADMFSIEVVGRASHGSRPWDGVDPIVAASQIVIGLQTIVSRQVD
                     ISKLPAVLTIGKFDGGVRSNIISDKVNMYGTMRTFDPDVRTDMIDRMRRTAESIARAS
                     GATATLKMNAYRYPVTVNDPDLTARVLPTLRRVAGAERVVERPLEMGSEDFPYYTEHV
                     PGFFFFVGVTPPQEDLALAARNHSPRFYIDESALPVASRALAAIAVDYLQGGR"
BASE COUNT          548 a          940 c          863 g          507 t
ORIGIN      
        1 aaccgggcgt gaatgcgaca gcgccgatcg ccacgcaagc cgcgcgtact gcagagttca
       61 atgtcatgat ggcttacctc ccccgaagct gaggcgcgct cagccgaata atcgttggtt
      121 ggaaggtgcg attcgacgcg caaagcggtg ccttccttgg tcgtataact tatatccgtc
      181 gcaacgaggg cgcgccaaat agtcgtaatg acggcgataa catgggctta atgcgtgctg
      241 aaaaagcaac agggcgcgag caatgaagca cacgcgaacg caatgcagcg acgcaccgcc
      301 gcactcgcga gtggcagatc catcgatcga cggatccgca ggcaagaaat cactcgcaag
      361 atcgttcagc tcgtcgatgc cagctcgtac aggaactcca cgtatcccat cgtcgcctcg
      421 cgcaggcctg ccaccttggg atcgttgctt tcgatgagat agaactcgtc cggcgcgtga
      481 gcgcgaccgc cgtgacccaa gccgaactga ttgaacggct tgccgagcgg cgcaccagta
      541 aacatgtatc cgggatatga gcccgcgatg cgcggcacga tcgaatgagg aacgcccagc
      601 cgcgcgagga cagccttctc ggcgcgaatc aaagcactgt cttccgccgt ctgagtcgga
      661 tcgtaggcgc caccgacttt gatctctatg tcggtgaatc cgcgcttgtc gagatgcgcc
      721 ctgagcttcg acagacaatc cttggcggtc atatccggca ccagccgcat gtccagcttc
      781 gcagtcgcgc ggcccggaag aatggtcttc ccgcccgccc ccgtgtagcc ggccaccaag
      841 ccctcgatgt tcaccgtcgg ccgtgcagcg ttcgcctcga gcgcctcgcg ccaggacatg
      901 tcgttgatcc agcgcttgat gccataggcg cgcttgaggt cggcctcgtt gctgttgcgc
      961 gccgcttgcg cgatcagctc gcgctctctt tcggtcagcg gccggtagtg ctcgagccac
     1021 ccctcgatgg cgggcgtgtt accgccatcc cgcaccaagg tgctcaacgc atcgatcagg
     1081 cgccacacag ggctgtcgac ggaggccttg aagccggagt gcacatcctg cgtcgccccc
     1141 cttccccacc gctctgcgct cgcgatcagc tcgcattcga tgatgccctt cgcaccgagg
     1201 cagatgggaa tctcgccgcc cagcccctgg agcgaggtgg gcatgaaaac cgcgtcggca
     1261 cgctggaact cgcgcagaac ctcctcgtcc ttgaagacgt tggtgaagtt gggcgatccg
     1321 atctcctctt cgccctccgc cagcaagacc aagttcaccg gcaactgcgc tccggcggaa
     1381 tgaaatgcat ggatcgccgc gagaaacgtc gcttccggcc ctttatggtt gatcacgccg
     1441 cgaccgcgca gcgcgcgtcc gagcggatga tcgaacatcg tcggctcgag cggcggtgag
     1501 ctccactctg gctcatcgaa ctgctttaca tcgtacatga agtacaggcc gacggtgcga
     1561 cgcgccccga catcgagcac gccgaaaacg gccggactcc cgcccgttgg cactttgcgc
     1621 acccggctga agcccgcttc cttcgcgagc cgcgccatat agtccgcgcc ctcttcgatg
     1681 ttgagcccct cggcggcgac ggtcggcagg cgaatccaat cctggatgcg tcgtagtgac
     1741 gcttcgaatc ccgcctctac agcccgtttg atagcgctgc gcttgcccgc gtcgagcgcg
     1801 gcgaaagctc cgggcgccga caaagcggca gcagaggcca gagcgccctg gatcaaagtg
     1861 cgtcgattca tcgtccgccc tgcaaatagt ccaccgcgat ggcggccaac gcgcgacttg
     1921 cgacgggcag cgccgattca tcgatgtaga aacggggtga atgattccgc gccgccagag
     1981 cgagatcctc ttgcggcgga gtgacgccga cgaagaagaa gaaccctgga acgtgctcgg
     2041 tgtagtacgg gaagtcttca gagcccatct cgagcggccg ctcgacgaca cgctcggcgc
     2101 ctgcgacgcg acgcagagtc ggcaacacgc gcgcggtgag atccgggtcg ttgacagtga
     2161 ccggatagcg atacgcattc atcttcaacg tggctgtggc gccgctggct cgagcgatgc
     2221 tctcggccgt tcgacgcatg cggtcgatca tgtcggttcg cacatcgggg tcgaacgtgc
     2281 gcatcgtgcc gtacatgttg accttgtccg agatgatgtt gctgcgcacg ccgccgtcga
     2341 acttgccgat cgtgaggacc gcaggcaatt tcgagatgtc gacctggcgg ctgacgatgg
     2401 tctgcaagcc gatgacgatc tgcgaagcgg caacgatcgg atcgacgccg tcccacgggc
     2461 gcgagccgtg cgaggcccgg ccgacgactt cgatgctgaa catgtcggcc gccgccatga
     2521 acggtccgga gcggtagccg atcgtccctg ccggcattcc agcaaagacg tgcaggccga
     2581 acacggcctc gggcgcagga tcccgaaaga tgccctgcct catcatgagc tcggcgccgc
     2641 cttcctcgcc ttccggagga ccctcctcag cgggctgaaa gacgaacaag acattacccg
     2701 gcaagtcccg acgcaggctg gtcagcgcct cagccacgcc catcaagttg gcagtgtgcg
     2761 catcgtgacc gcacgcatgc atcacaccca ctttcttgcc gaggtactcc gcggttaccg
     2821 tcgacttgaa gggaacgtcg atttcctctg taaccgga
//
LOCUS       DASVDU010000172        11501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_139351, whole genome shotgun sequence.
ACCESSION   DASVDU010000172 DASVDU010000000
VERSION     DASVDU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11501
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139351"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF226_10870"
     CDS             complement(<1..266)
                     /locus_tag="VF226_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074532.1"
                     /translation="MTGANEIITPDELRHIVEEKEMERVREAAAHLKKKEDEERQLRE
                     MFMEREIHPEAKARVSEVIRRAAERGQHEVMAIRFASAWCTDKGR"
     gene            671..3007
                     /locus_tag="VF226_10875"
     CDS             671..3007
                     /locus_tag="VF226_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HEX7074533.1"
                     /translation="MATQSRIGSGRNDTKSAAKGADAAPVGLWGAGVEYAIDAWQRNI
                     LFLDVLRQRGNQYLEHLAESAPHVLSFDAELVMDGRSLRRPVNYGLVRIAPPKGVIIE
                     PKMRPFVVVDPRAGHGPGIGGFKADSEIGVALRAGHPCYFIGFLPEPVAGQTIEDIMA
                     AEAMFLERVIELHPETEEKPCVIGNCQAGWAIMMLAAVRPDLFGPILMAGTPLSYWAG
                     VHGKNPMRYTGGLLGGSWLTALMSDLGNGKFDGAWLVRNFESLNPANTFWTKQYNLWS
                     KVDTEAPRYLGFERWWGGHVDLNADEIQFIVDELFIGNKLASGEIVTRDGVRIDLRNI
                     RSPIICFCSRGDDIAPPQQALGWITDLYAKDTDIIAHGQTIVYALHESAGHLGIFVSG
                     SVAKKEHAEFASNIDLIDVLPPGLYEAVLIPKKPDAGNVEFAEGDWIVRFERRTLDDI
                     RALGGNDPDDERRFAAVRRLSEINNGLYRTFAQPFMQALANDQTMEWLRRLNPARLQF
                     ELLSGRNPLMQPVANLAAQVREQRRPVAEDNPLTRLQQQISEQIESALDAYRDLRDSW
                     YEQTFMAFYGSLPFQALLGLQASDTPPRRRPGDEPEDRAFVRQKIAELKRSVAEGDHR
                     TAAIRSLLYVGFGSDLSEGAIDERAFNALQQIKAERGGSMTLAEFKRIVRQQSLMLLL
                     DEQSALAALPGMLGSDPAERDGTVKALRRILSAVDGHDRAHQERLSTVEELFGQKGAE
                     AKARTEDRSGKEAGGRGMRPAARQPRGRRRAAAAALRR"
     gene            complement(3086..4132)
                     /locus_tag="VF226_10880"
     CDS             complement(3086..4132)
                     /locus_tag="VF226_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7074534.1"
                     /translation="MGLVMLLLLTVGCFTVLWPFLAAILWAGILAFSTWPIYRRVEHL
                     AGERRGLAATLMTLGIAFILVVPLAILGSQISDDVIRLAESARTTLQTGPRPPPDWVY
                     NIPLVGHRLAEFWQSVAYDGAALAAAARPYVGPARNWLLAQSTVLAGGILEVSLSVFT
                     AFFLYQSGPVVVGALDAILGRIAGTRGHTLLLLAGGTVKGVVYGVLGTALAQAILATL
                     GFWVAGVPGALFLGLLSFFLTLVPMGLVLVWVPAVIWLFIQDAALWGILLAIWMVFVS
                     GIENVLRPYLISKGGELPFLLILLGILGGIIAFGLIGIFLGPTLLALGYSLISEWARN
                     DPSASTSETRGLDD"
     gene            complement(4309..4746)
                     /locus_tag="VF226_10885"
     CDS             complement(4309..4746)
                     /locus_tag="VF226_10885"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074535.1"
                     /translation="MILRTQSEYRDRWNGHRWMWLISALAVAGASAPVAEAASSERLV
                     EAFRNLDANADGQVSAVEFDLRKIQVFALRDANGDQMLQRSEVDLDSDQFRAADQNGD
                     GQISGLEFVDSPIGQFEIYDTDRNGSLTLDELTTKPVSSTSSP"
     gene            complement(4883..6442)
                     /locus_tag="VF226_10890"
     CDS             complement(4883..6442)
                     /locus_tag="VF226_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074536.1"
                     /translation="MPRFRRRTARHGKRPGSILLGHLGSKSCDCSALGVIACAVFLLL
                     IPTVEGAEKVRWVRLSAVMSELSNEPGFVDGLLRRLGHDPDAGGILGPDEIKRLREAI
                     LGKHWETLDRFPGLTVAGLGRSVRLAGLAQRHLVDRTDTGAPNAAGARAAARGVDVVE
                     SLGIPAKGPASRPDGLLTPLGFGLAWGDRVDVGRMAHYADSLRLANVLNRLALNPAEG
                     QSAPRYRVSTAADMADSPQALLAALARAGHKVMVLDARYFANFGDLIYKGQDVLTPFW
                     MDTKITIPGTKRSLLVPVGHSQHEIIVRGPTVKADLSFYFGIDGKAEFRPTATRNQSW
                     VLGRIARTYRGEQALEVVRLAGEIVKAYDAVKRAHPDLPFGGYFALGVCNDVNAMIEL
                     HMQGETTLFPLTLDSDYFPGEGEVERLARQLPVDRGGRPPSPSRVFGSLPVVDLAQLP
                     LPELRTDLETVRDAWQRGELNDTGSWRIMPWALLLLTGFAVAALIYFRKRYRPRPAQP
                     PARRQTRPHRP"
     gene            complement(6611..6922)
                     /locus_tag="VF226_10895"
     CDS             complement(6611..6922)
                     /locus_tag="VF226_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074537.1"
                     /translation="MPKRTILRLIRGGKAVEGPAQSNRSPSQTSPEQESLEHCRARLA
                     DLNDPALKSLLDDYDIAVDSLRRWQMSHHPHAAERVLEYRTLISEIEVEILAVLDKRT
                     T"
     gene            7275..7460
                     /locus_tag="VF226_10900"
     CDS             7275..7460
                     /locus_tag="VF226_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074538.1"
                     /translation="MAYACVLRAYEHRRLMGLSGLLAVRACLERAVQEEPEYAEARAM
                     PASCRSRRFARPAVIGS"
     gene            7666..8364
                     /locus_tag="VF226_10905"
     CDS             7666..8364
                     /locus_tag="VF226_10905"
                     /inference="COORDINATES: protein motif:HMM:NF023197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HEX7074539.1"
                     /translation="MVLRQVVYGVCVVALVGGCGATQQARDVETSGFLGEDYALLRQG
                     GENESLLTYRAPDADWRAYDKIQLDPVTVWTRDGSAFKDASAAERQQLADDLYAAVYK
                     ELAEDYQMVSAPGPGVMRIQVALTDAQASNPMLDTVSTVMPAGLAVSGATGLVTGKPG
                     FVGEARAEAKVTDATTGELLGAAADRRVGGKSLGGSIDSWDDADQAFQYWAEQFRYRL
                     CTQRGDTDCEPPEA"
     gene            8397..10397
                     /locus_tag="VF226_10910"
     CDS             8397..10397
                     /locus_tag="VF226_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074540.1"
                     /translation="MPAEATHVVLRQSRRCGTLVSLLPWEDHALRQALGACLLLMLLV
                     VGGCATPVGVKHVGQEAVHRELTGNVLSTGELSQFTENALRQRGLDQVAETDAPAALA
                     ALHEATVKGNLRPDDLFALAELSFAHAEAYGGRPYYLASAIYAYAFLFPEGMGEPPSP
                     FDPRFRWAVDLYNRALTNAFESPDGRAFEPRGGRFGLPFGKLEVAFDKSELLWSGRRL
                     TAFVPVGEFELWGLKNRYRYPGLGAPLAAGAVPLRQETGFQVAQRLKLPATALLRIAN
                     ARRQLAESNGGDFRASLELYPLSTTEEVQIAGGPVPLETEPSAALAYTLSDPAVWATE
                     LRGFLLGDLLRSKPSQLVSLQPYVPGRIPVVLVHGTASSVGRWADMVNDLLSDPRIRK
                     RFQFWFFSYETGNPVPYSAMVLRDALRDAVARLDPQGRDPALREMVVIGHSQGGLLAK
                     MTAVDTGDRLWRSFSRRPLEELKTRPETRDLLQRALFVQPEPSVRRVVFIATPHGGSY
                     LTEYSVTRLIARLVRLPFSAASAVADVVTNNPDAFTYDPRQVRAGSIYGMTPGSPFIT
                     GLAQIPVAPGIAANSIIAVDGDGPLEDGSDGVVAYRSAHIKGVESELVVRSPHSCQSD
                     PATIAEVHRILLLHAEEACAERGIGCNGTPVQKEQLVVSAAQ"
     gene            10478..11026
                     /locus_tag="VF226_10915"
     CDS             10478..11026
                     /locus_tag="VF226_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074541.1"
                     /translation="MSTVHSALRTTLLVVLMFGFGGTEPGSAVEQRSVDAFAAWQGRG
                     QVYQTGPDAATFVGAFSGRFYLESGQGPLDASYLTCPGIIEINLKDGAQTGQGRCTIT
                     AEDGARVFAQWRCSGRHLAGCDGEFTLTGGSGRLTGIKGGGPMVVRSGLAEIVAGMPG
                     NMVQEAAAGVIIWRGLRYSLAE"
     gene            11095..>11501
                     /locus_tag="VF226_10920"
     CDS             11095..>11501
                     /locus_tag="VF226_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEX7074542.1"
                     /translation="MFRSQCALRACIAMAIGAASVWFLEIASAQVASPIVYPARGQSL
                     EQQSADEAACRDWATQQTGVVPYYGAPPPTAAAAPTGGVLRAAARGALLGTVGGAIAG
                     DTGKGAAIGAGVGATAGFMRQNRQRREQAAMEN"
BASE COUNT         2090 a         3553 c         3798 g         2060 t
ORIGIN      
        1 cgccccttat cggtgcacca cgcgctggcg aagcggatgg ccataacctc gtgctggccg
       61 cgctcggcgg cgcggcggat gacctccgag accctggcct ttgcttccgg atggatctcg
      121 cgctccatga acatttcgcg cagctgcctt tcctcgtctt ctttcttttt caggtgcgcc
      181 gctgcctcgc gaaccctctc catttccttt tcttcgacga tatgccgaag ctcgtctggc
      241 gtgatgatct cgttggcgcc ggtcatcctc gttctccttg gcttgcgcgc ctgctgccgg
      301 gcttttgcgc gagcgcacag gcgcggacga ttcgccgtca gaagggtaag ccagcgcttt
      361 cggccgatgg caggtagtct acggtaagtt acgcgcgctg cgatgccgcg aggcacgggc
      421 ctcccccggc gctgcttcgc gcgtccgaat gaggcggcct atgcgatcga ccctgggctg
      481 ctccgccttg agctcgcctc ggcgcggcag gcgggccgtg tcgacagagc cggcaaactg
      541 ccctagggag accgtaagtt acagtaacga actgctgttt gccgcagccc gtccttatcg
      601 ttcagccacc gagagagttg tcggttgtgc cttgttgccc gcgtagtgcg cgtcggaagg
      661 aaagtttagg atggcaacgc agtcaagaat cggatccggc cggaacgata cgaaatcagc
      721 agcaaaaggt gcggatgctg cgcccgtcgg gttgtgggga gccggtgtgg aatacgcgat
      781 cgatgcctgg cagcgcaata ttttgttcct ggacgtgctt cggcagcggg gcaaccaata
      841 tctggagcat ctggccgaaa gcgcaccgca cgtcctgtcc ttcgatgcgg agcttgtcat
      901 ggacgggcgc agcttgcggc ggccggtcaa ttacggcctc gtgcggatcg cgccaccgaa
      961 aggcgtgatc atcgaaccta agatgcgccc gttcgtcgtc gtcgatccgc gcgccggaca
     1021 cgggccgggg atcggcggct tcaaggccga cagcgagatc ggggtcgcgc tccgggccgg
     1081 tcatccatgt tacttcatcg gttttttgcc cgagcctgtg gccggccaga ccatcgagga
     1141 catcatggcc gccgaggcca tgttcctaga gcgcgttatc gagctccatc ccgaaaccga
     1201 ggagaaaccc tgtgtcatcg gcaattgtca ggccggttgg gcgataatga tgctcgctgc
     1261 cgtacggcct gacctgtttg gcccgatcct gatggcgggc acgccactct cctactgggc
     1321 cggcgtgcac ggcaagaatc cgatgcggta cacgggaggc ctcctcggcg gaagctggct
     1381 caccgcactc atgagcgatc tcggcaacgg caaatttgac ggtgcttggc ttgtcaggaa
     1441 tttcgagagc ctcaatccgg cgaacacgtt ctggaccaaa cagtataatc tctggtccaa
     1501 ggtggacacg gaagcgccgc gatatctggg cttcgagcgg tggtggggcg gtcacgttga
     1561 cctgaatgcc gacgagatac agttcatcgt cgacgagcta ttcatcggca acaagctcgc
     1621 gagcggggag atcgtgaccc gggatggcgt gcgcatcgat ttgcggaaca ttcgatcgcc
     1681 gatcatctgc ttttgctcgc gcggagatga catagccccg cctcagcaag cgctcgggtg
     1741 gattaccgat ctctatgcga aggacaccga tatcatcgcg catggccaga ccatcgtcta
     1801 cgcgctccac gagagcgcgg gtcaccttgg cattttcgtc tcgggttcgg tcgccaagaa
     1861 agagcacgcc gagtttgctt cgaacatcga cctgatcgac gtcctcccgc caggccttta
     1921 tgaggcagtc ttgattccga agaaacctga cgcgggcaac gtggaattcg cggagggtga
     1981 ttggatcgtg cgcttcgagc ggcgcacgct cgacgatatc cgggcgcttg gcggcaatga
     2041 ccccgacgac gagcggcggt ttgccgctgt acggcggctg tcggagatca acaacggtct
     2101 ttaccgaacg ttcgcccagc cattcatgca ggcgcttgcg aacgatcaga ccatggaatg
     2161 gctgcgtcga ctgaaccccg cccggctgca gttcgagctg ttgtccggcc gcaacccgct
     2221 gatgcagccg gtcgccaatc tcgcggcgca ggttcgcgag caacggcgcc ctgtcgccga
     2281 agacaaccca ctgacgcggc tgcagcagca gatctcggag cagatcgagt ccgcactcga
     2341 cgcttaccgt gatcttcggg atagctggta cgagcaaaca ttcatggcgt tctatggctc
     2401 gctaccattc caggcactcc tcggcctgca agcgagcgat acgccgccgc gccgccgccc
     2461 gggcgacgag ccggaagacc gcgcctttgt ccgccagaag atcgccgaac tgaaacggtc
     2521 ggtcgcggag ggagatcata ggacagcggc aatccgctcg ctgctgtatg tgggcttcgg
     2581 ttcggacttg agcgaaggtg ccatcgatga acgtgccttc aacgcacttc aacagatcaa
     2641 agccgagcgc ggcgggtcca tgacactcgc cgagttcaag cgcatcgtgc ggcaacaatc
     2701 cttgatgctc ctgctcgatg agcagagcgc acttgcggcg ctgccgggca tgctggggtc
     2761 ggaccctgcg gaacgggacg gaacggtcaa ggcgctccgg cgcatactct ctgcggtcga
     2821 cggacatgat cgcgcgcatc aagagcggct ctcgaccgtt gaggagctgt tcggccagaa
     2881 aggggctgaa gcaaaggcgc gcaccgagga taggtccggc aaagaggcgg gcgggcgcgg
     2941 gatgcggcct gctgccaggc agccgcgagg cagacgacgc gccgcggcag ccgcactgag
     3001 acggtgaggg gcgttcggcc cgatcgttca ggaaagaacg aaacagtgag aaaggcttgg
     3061 aggagcggtc ctgcgtgcta gcgggctaat cgtccagacc tcgggtctcc gaggtcgatg
     3121 cgctagggtc atttcgcgcc cactcgctga tcaggctgta accaagcgcc aacagcgtcg
     3181 gcccaagaaa gatcccgatg aggccgaacg cgataatgcc cccaagaatg ccaagcagga
     3241 tcaagaggaa gggaagttca ccccctttgc tgatcagata cggcctgagg acgttctcga
     3301 tcccgcttac aaagaccatc cagatcgcga gcaggatgcc ccagagcgcg gcatcttgta
     3361 tgaagagcca gatcacggcc ggtacccaga caagcacgag gcccatcgga acgagggtca
     3421 ggaaaaagga caacaagccc aggaacaagg cgccgggaac gccggcgacc cagaagccga
     3481 gggtggcaag gatcgcctga gcgagggctg tgccaagtac accgtacacc acgcccttga
     3541 ctgttccacc cgcaagcagc aggagcgtat ggccacgagt gccggcaatc cggccgagga
     3601 tcgcatcgag ggcgccgacg acgacggggc cggactggta gaggaagaag gcggtgaaaa
     3661 cgctgaggct gacctcgaga atgccgccag ccagcacggt gctctgcgcc aggagccaat
     3721 tgcgggccgg accgacataa ggccgggccg cggctgccaa ggcggcaccg tcgtaagcaa
     3781 cgctttgcca gaactccgca agccgatgtc cgacaagcgg aatattgtaa acccagtccg
     3841 gtggcgggcg cggtccggtt tgcagcgtcg tccgagcgct ctctgcgaga cggatgacat
     3901 catcggaaat ctgcgatccg agaatggcca ggggcacgac caggatgaat gcgatcccga
     3961 gtgtcatcag ggtggccgcc agaccgcggc gctccccagc gaggtgctcg acgcgccgat
     4021 agatcggcca ggttgaaaac gccagaatac cggcccagag gatcgctgcc aggaacggcc
     4081 agagcacggt aaagcaccca accgtcaaca acagaagcat tacgaggccc atcgcctttc
     4141 ccacataacc atccggcttg gccgacccgg agccagccaa gtcacgaggg gccgaagggg
     4201 cgaagtcttg atgcattcgg taggaccggt ccgttggctg ttggttcggc tggtcgattg
     4261 gctcccggat tcaaggtcaa ctgcccggga atctggacgg ttgccgggtc acgggctcga
     4321 ggttgacgaa accggcttcg tcgtcagttc gtccaaagtg agagagccat tcctgtccgt
     4381 gtcgtaaatt tcaaattggc cgattggcga gtcgacgaac tccagaccgg agatctggcc
     4441 atcgccgttc tgatctgccg cccggaactg gtccgaatcg aggtcgactt cgctgcgctg
     4501 aagcatctgg tcgccattgg catcgcgcag agcgaaaacc tggatctttc tcaggtcgaa
     4561 ctcgaccgcg ctgacttgcc catccgcgtt ggcatcgagg tttcggaagg cctcaacgag
     4621 gcgctccgag gatgcagcct ccgcgaccgg agcagaggca cctgcgactg caagggccga
     4681 tataagccac atccagcggt ggccgttcca tcgatcccgg tattccgact gggttctgag
     4741 tatcatcgaa cactccctgc cttcggtaag aatcgctatc cgatcccgaa ggcctatcta
     4801 tcagcgggaa cgttggagcg tgggacaatt catacgttca ttcgggcatc ttcctatctg
     4861 aagtcgagaa ttttacggta aattacggtc ggtgcggtct tgtctggcgg cgggctggcg
     4921 gttgggccgg ccgtggcctg tagcgctttc gaaaatagat caaagcggcg acggcgaacc
     4981 ccgttaagag aagcagcgcc catggcatga tgcgccagga gccggtatcg ttgagttcgc
     5041 cgcgctgcca cgcatcccgc acggtctcaa gatccgttcg caattccggt agcggcagtt
     5101 gggcaagatc gacaacgggc agggaaccaa agaccctgct cggacttggc ggacggccgc
     5161 ctctatcgac aggcagctgt cgggccagcc gctcgacctc accttcgcca gggaaatagt
     5221 ccgaatcgag ggtgagcgga aacagagtgg tctcaccctg catatgaagc tcgatcatcg
     5281 cgttgacatc gttgcagacc cccagtgcaa agtagccgcc gaacggaagg tccgggtgtg
     5341 cccgtttgac ggcgtcgtag gctttgacga tctcgccggc gaggcgaacg acctccagcg
     5401 cctgctcgcc gcggtaagtg cgtgcgatgc ggcccaagac ccatgactgg ttccgcgtgg
     5461 cggtcggccg aaactccgcc ttgccgtcga tgccgaaata gaaggagaga tcggctttca
     5521 ctgtcgggcc gcgcacgatg atctcgtgct gggagtggcc gacaggaacg agcagcgagc
     5581 gcttggttcc cggtatcgtg atcttcgtgt ccatccagaa cggagtgagg acatcctgtc
     5641 ccttgtagat gagatcgccg aaattggcga agtaacgggc gtcgagcacc ataaccttgt
     5701 gaccggcccg cgcgagagcg gccagcagcg cctgcgggct gtctgccatg tccgccgccg
     5761 tggataccct gtatcgcggc gcggactgcc cctcggccgg attgagtgcc agccggttca
     5821 ggacattggc caaacggagg ctgtcggcat aatgcgccat ccgaccgacg tcgactcggt
     5881 cgccccacgc aaggccaaag ccgagcggcg tgagcaggcc gtccggcctc gaagcaggac
     5941 ccttggccgg aattccgagg gactcgacga cgtcaacgcc cctggccgcc gccctggccc
     6001 cggcggcgtt gggagcaccg gtgtcggtcc tgtcgacgag atgcctttgt gccaaaccgg
     6061 cgaggcgtac cgagcggcca agtccggcga cggtcaggcc gggaaagcgg tcgagcgttt
     6121 cccagtgctt gcccaagatc gcctcgcgca accgcttgat ctcgtccggt ccgagaatgc
     6181 cgccggcatc cggatcgtgg ccgagtcgtc tcaggagtcc gtcaacgaag cccggttcgt
     6241 tcgacagctc actcatcacg gccgagaggc gaacccagcg tactttctcg gctccttcga
     6301 cggtcgggat gagcagcagg aagactgcgc aagcgatcac gccgagggcg gagcaatcgc
     6361 agctctttga tccaagatgg ccgagcaaga tgctgccggg ccttttcccg tgccgggccg
     6421 tcctccggcg aaaccttggc atcgccgagg tcctgtcaac cgccactctc gcggcgctga
     6481 tcaaatggct gcgtgccgag aatgcggcgt ccacaagcga cgggtaaccc tttcccgcat
     6541 gacgcggagg gggcgctgcg ctggcttgtg tcatgccaat tgccgaacgc ggcagtttca
     6601 tcgcggtgcg tcaggtcgtt ctcttgtcca gcacggccaa gatttctact tcgatctccg
     6661 agatcaaggt ccggtattcc aacactctct cggccgcgtg aggatggtga gacatctgcc
     6721 accgacggag gctatcgaca gctatgtcgt aatcgtcgag aagtgacttc agcgccgggt
     6781 cgttcaggtc cgctaggcgg gcgcgacaat gttcaaggct ctcctgctcg ggtgatgttt
     6841 gtgacggaga ccgattcgat tgcgccggcc cctccacagc ctttccgcca cggataagtc
     6901 tcaggatggt gcgcttcggc atttccgcct cattgcgccg ctggcccatt tggctgcgtc
     6961 acgatggatg cttcgttacg aggcgcaggt gaagaatcac atgtatggtt tcgaagaaga
     7021 ggtaactttc agtaacaacg gagcgttctc ggggcgatga atctgccagg cgatttgcga
     7081 tggcgaccac cgcctttggt ggggttgaaa tgggccacgt tgttttacgc tcaacgcgga
     7141 gttctcttgg tgcgttcgga ggcggtggtt gcgcgatgga tgacagcatt atcggcctgg
     7201 cccgcaagca aagccgtcat aaggtcaaga gagtagcggc ggtaccgatg ggcggttgag
     7261 cgccggagag cctgatggcc tacgcgtgcg tgttgcgggc ctacgagcac cgtcgcctga
     7321 tgggcctttc cggcctcctc gcggtgcggg cctgcctgga gcgcgccgtc caagaggaac
     7381 cggaatacgc cgaggcccgg gccatgccgg catcgtgccg gtctcgacgt ttcgctcgtc
     7441 cggccgttat cggctcgtga tccggcacac gaagccctcg gttcagacga gcctcgcgcg
     7501 agcggttgcg taacttaccg taacattctc ccatttggat ctgattcgcc tgaatctgct
     7561 tcggtgaaga cttgccgtcg tgaacacaac cggtggcgac acgaaactcg ggtcgatccg
     7621 gctgcggcga gctctcgtga aagcgataaa ggcggaggag cgaagatggt tttgaggcag
     7681 gttgtgtatg gggtctgcgt ggttgcactg gttggcggct gcggcgcgac gcagcaggcg
     7741 cgcgatgtgg agaccagcgg ttttctcggt gaggattacg cgctgctgcg ccagggcggc
     7801 gagaacgagt cgctgctcac ctatcgcgct cccgatgccg actggcgcgc ctatgacaag
     7861 atccagctcg acccggtgac ggtgtggacc agagacggtt ccgcgttcaa ggatgcgtcc
     7921 gcggcggagc ggcagcagct tgccgacgat ctctacgccg ccgtctacaa ggaattggcg
     7981 gaggactacc agatggtctc ggcgcccggc ccgggggtga tgcgcatcca ggtcgcgctg
     8041 accgacgcgc aggcctcgaa cccgatgttg gacacggtgt cgaccgtcat gccggcgggc
     8101 ttggccgttt cgggggctac gggtctcgtg accggcaagc cgggcttcgt tggcgaggca
     8161 cgggcagagg cgaaggtgac ggacgcgact actggcgaac tcctgggtgc cgcggctgac
     8221 cggagggtcg gcggcaagtc gctcggcgga tcgatcgatt cctgggatga tgcggaccag
     8281 gcattccagt actgggcaga acagttccgc taccgtctct gcacccagcg cggcgacact
     8341 gactgcgagc cgccagaggc ctgaacgcaa aggtcgcacc atctgctgag gcagacatgc
     8401 ccgccgaagc tacccatgtt gtgctacggc aatcccgccg ctgcggaacg ctcgtgtctc
     8461 ttttgccatg ggaagaccac gcgttgcggc aggcgctggg cgcgtgcctt ctgcttatgc
     8521 ttcttgtggt gggtgggtgc gcgacgcccg tcggggtcaa gcatgtcggc caggaggcgg
     8581 ttcaccgaga actcacgggt aacgtccttt cgaccggcga gttgagccag ttcaccgaga
     8641 acgcgctgcg ccaacggggc ctcgatcagg tggcggagac ggacgccccg gcggctttgg
     8701 ccgcgcttca cgaggcgacc gtcaagggca accttcggcc ggacgatctc ttcgcgctcg
     8761 ccgagctttc ctttgcccat gcggaggcat acggcggtcg gccctattat ctcgccagcg
     8821 cgatctatgc ctacgccttc ctttttccgg aggggatggg cgagccaccg agcccgttcg
     8881 acccccggtt ccgctgggcc gtcgatctct acaaccgcgc cctcaccaac gcgttcgaat
     8941 caccagacgg gcgcgcgttc gagccgcggg gcggccgttt cggtctgccg ttcggcaagc
     9001 tcgaagttgc gttcgacaag agcgagcttc tgtggagcgg gcgtcgtctc acggccttcg
     9061 tgccggtcgg cgaattcgag ctgtggggac tgaagaaccg ctaccgctac ccgggcctcg
     9121 gggcgccgct ggcggcgggc gcggtcccgt tgcgccagga aaccggcttc caggtcgccc
     9181 agcgccttaa gctgccggcc accgcgttgc tgcgcatcgc gaatgcccgc cggcagcttg
     9241 ccgaatcgaa tggcggagac ttccgagcct cgttggagct ctatcctttg tcaacgacgg
     9301 aggaggtcca gatcgccggt ggtccggttc cgctggagac tgagccgtcg gcggcgctcg
     9361 cctacacgct cagcgacccg gcggtctggg caacggagct tcgggggttc ctgctcggcg
     9421 accttcttcg cagcaagccg agccaactgg tctcgcttca gccttacgtg cccggccgca
     9481 tccccgtcgt gctggtccac ggaacggctt cgagcgtcgg gcgctgggcc gacatggtga
     9541 atgacctgct gagcgatcca cgcatccgta agcggttcca gttctggttc ttcagctatg
     9601 agaccggcaa cccggttccc tattccgcta tggtcctgcg cgacgcgctg cgcgacgccg
     9661 ttgcccggtt ggatccgcag ggtcgcgacc cggcgctgcg cgagatggtg gttatcggcc
     9721 atagccaggg cggcttgctg gcgaagatga cggccgtcga caccggcgac cggctgtggc
     9781 gcagcttcag ccgccggccc ctggaggaac tcaagacgcg gcccgaaacc cgggatctgc
     9841 tgcagcgggc gctcttcgtg cagccggagc cgtccgtccg ccgggtggtg ttcatcgcca
     9901 cgccgcacgg ggggagctat ctgacggagt actcggtgac ccggctgatc gcgagactgg
     9961 tccggttacc cttcagcgcg gcatcggcgg tcgcggatgt cgtcacaaac aatcccgacg
    10021 cgttcaccta cgacccgcgg caggtgcggg cgggcagcat ctatgggatg actcccggca
    10081 gtccgttcat caccggcctc gcacaaatcc cggtggcgcc cgggattgct gccaactcga
    10141 tcatcgccgt cgacggcgat gggccgctcg aggacggcag cgacggcgtg gtcgcctacc
    10201 ggagtgcgca catcaaaggc gtggagtccg aactcgtggt ccgctctcct cactcctgcc
    10261 agtcggatcc ggcgacgatc gccgaagtgc atcggatcct tctgctccat gccgaggagg
    10321 cctgcgcaga acggggcatc ggttgcaacg gaaccccggt ccaaaaggag cagctcgtgg
    10381 tcagcgcggc gcaatgagcc tcgacaggcc accgggtcgt atccgctgtc agcgtgacgg
    10441 cgatcgcgga caagcgccag aaagaaaagg agcagacatg agcaccgtcc atagcgcctt
    10501 acgcaccact ctcctcgttg tcttgatgtt tggcttcggc ggtactgagc ccggcagcgc
    10561 ggtggaacag cggagcgtcg acgccttcgc cgcctggcag gggcgcgggc aagtgtacca
    10621 gactggcccg gatgccgcga ccttcgtcgg agcgttcagc ggccgttttt atctcgaaag
    10681 cgggcagggc ccgctcgacg ccagttacct gacctgcccc ggcatcatcg agatcaacct
    10741 gaaggatggc gcacagaccg gccaagggcg gtgtacgata accgcggagg acggggcccg
    10801 cgtgttcgcc caatggcggt gcagcggccg tcaccttgcc ggctgcgacg gcgagtttac
    10861 gctgaccggt ggctccggtc gcctgacggg gatcaaaggc ggcgggccga tggtggtgcg
    10921 cagcggcctg gccgagattg tcgctgggat gccagggaac atggtgcagg aggccgcggc
    10981 gggcgtgatc atctggcgcg gccttcgtta ctcgctcgct gaatgaacag tgtcggtgca
    11041 cctatcggcc gtgaagcgga tgagggcgcg aaacgtttgg acaggaaggt caggatgttc
    11101 cgttcacaat gcgccctgag ggcctgtatt gcgatggcga tcggcgcggc aagcgtgtgg
    11161 ttcctcgaaa tcgcctctgc ccaggtcgcg agtcccattg tctatccggc gagggggcag
    11221 agcctcgagc agcagtcggc ggacgaggcc gcgtgccgtg actgggcaac gcagcagacc
    11281 ggcgttgtgc cctactacgg cgcaccgccg ccgaccgcgg cagccgcgcc aaccggcggc
    11341 gtcttgcgcg ccgccgcccg cggcgcgctg ctgggcaccg tcggcggcgc catcgcggga
    11401 gacaccggga aaggcgcggc catcggggcc ggcgtcggcg cgacggccgg cttcatgcgc
    11461 cagaaccggc agcggcgcga acaagcggcc atggagaacc a
//
LOCUS       DASVDU010000173        12481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_446798, whole genome shotgun sequence.
ACCESSION   DASVDU010000173 DASVDU010000000
VERSION     DASVDU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446798"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..599)
                     /locus_tag="VF226_10925"
     CDS             complement(267..599)
                     /locus_tag="VF226_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HEX7074543.1"
                     /translation="MKLPTLPFTVTDWDSIEPTVHPGETGEARWRTFTVGDLRVRIVE
                     YSPGYLADHWCDRGHVLYVLEGELKTELRDGRTFTLKPGMSYQVSDHGDAAHRSSTRT
                     GAKLFIVD"
     gene            complement(639..2051)
                     /locus_tag="VF226_10930"
     CDS             complement(639..2051)
                     /locus_tag="VF226_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7074544.1"
                     /translation="MATAAVPEPARETIETAIAVLRQRFGDRLQTGEAIRRQHGHTLT
                     WISNQPPDAVVWPESTDEVREIVHIAASHRVPLIPFGAGTSLEGHVNAPFGGISVDLS
                     RMNRVLQVNDRDLDCLVQAGVSRKQINDHLRDMGLFFPIDPGAEEATLGGMASTRASG
                     TNAVRYGTMRENVLNLTAIMADGSVVRTARRARKSAAGYDLTRLLIGSEGTLGIITEL
                     TVRLYGIPEVILAAVCPFKTIEGACNAVIQSIQLGLGIARMELLDEVQIYAVNNHSKT
                     SFEESPTLFLEFHGTEVSARDQVEQFKEIALSEGAIRFDWAEKEEDRRRLWKARHDAY
                     WAVRSTWPGHDVLATDVCVPISRLAECVLETQKDIKEHGIIAPIVGHVGDGNFHTSPV
                     FAMNDPAARAKIEDFLKRLAERAIAMDGTCTGEHGIGQGKMEYLKSELGPGLDIMRQI
                     KRALDPLNIMNPGKIVALTE"
     gene            2481..2777
                     /locus_tag="VF226_10935"
     CDS             2481..2777
                     /locus_tag="VF226_10935"
                     /inference="COORDINATES: protein motif:HMM:NF025051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX7074545.1"
                     /translation="MVGRSGIALLATIAICGGAAFAQQTTPVPPGQSSTGQQQTRVSP
                     EQAIQMARNQGMVDVRELDRDGNVWEIEGHDAQGQEIEVKINILTGEVVSIERG"
     gene            2927..4054
                     /locus_tag="VF226_10940"
     CDS             2927..4054
                     /locus_tag="VF226_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007792251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX7074546.1"
                     /translation="MKTRAAIAFEKAKPLEIVELDLEGPKEGEVLVEIKATGICHTDD
                     FTLSGADPEGLFPAVLGHEGAGVVVDVGPGVTSVKKGDHVIPLYTPECRNCPSCLSRK
                     TNLCTAIRATQGQGVMPDGTSRFSYEGRMVHHYMGTSTFSNFTVLPEIAVAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGSTAIVFGLGGIGLNVIQGLRLVGADMIIGVD
                     INPDKKAWGERFGMTHFVNPKDVGGDLVAYLVNMTKRGADQIGGADYTFDCTGNVDVM
                     RTALEACHRGWGQSIIIGVAGAGQEIRTRPFQLVTGRVWKGTAFGGARGRTDVPKIVD
                     WYMDGKIQIDPMITHTLPLERINEGFDLMHSGKSIRSVVIY"
     gene            complement(4188..5156)
                     /locus_tag="VF226_10945"
     CDS             complement(4188..5156)
                     /locus_tag="VF226_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7074547.1"
                     /translation="MQGKVPPEQSAPRVYVGVDVCKDWLDVYLHPIGHQFRIASTPDG
                     LKRLKRHLAGLQVVVIVMEATAKYHRQPHRTLSAAGFAVAVVNPLRSRLFAEATGQLA
                     KTDRLDARVLAILAESLEPAATPPVAEALAALQELVRARAAAIADQTALTNQRAASQT
                     AFLKAELGRRLKAIATHIARLEAEIDRRIASDPALKRRYEIVRSIPGIGPVAAVALII
                     GLPELGTCSGKAAGLLAGLAPLACDSGATAGQRRIRGGRAHVRTALYFAAIAAARFNP
                     GLSGFYRRLRAAGKKPKVAITAVMRKLVVLANVLIREDRLWQAHQA"
     gene            complement(5454..6680)
                     /locus_tag="VF226_10950"
     CDS             complement(5454..6680)
                     /locus_tag="VF226_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7074548.1"
                     /translation="MGAERLEKKLAERLSELGHAGRLKGAESVITGVIPADGQKGPRF
                     LLEGYGAQPFLRMNSNSYLGLSLDAEVKAAEDSAVLKYGTGPGAVRFISGTWAPHVAL
                     ERRLAAFHDREAAMLFSSAYATVMGLLPQLITDRTAVISDELNHNCIINAIALGRPAE
                     KRIWRHLDLADLERHLTEAAKSCDRAIVVTDGVFSMRGDHAPLDAVRSIIDAHDANFA
                     ENAILVVDDSHGVGAFGRTGRGTEEVTGAKADLLVGTLGKAFGVNGGYVTGSATVIRY
                     LRETSPFYIYSNPITPAEAAAACKAVDIVDGPRGRQLLDHLREMTARFRHGLTALGLE
                     TLPGEHPVVPLMVRDTTRTATLVAHLRNAGILATGLNFPVVPKGDEEIRFQISADHTP
                     FDVDEALAALGAFAGR"
     gene            complement(6697..7683)
                     /locus_tag="VF226_10955"
     CDS             complement(6697..7683)
                     /locus_tag="VF226_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX7074549.1"
                     /translation="MPRASSSARSILVTGALGQIGSELVPALCDRYGSERVISSDLRM
                     EPLGEPFSGPSEQLDCTDLAQLQDVVRRHDVGTIYHLAAILSATAEAKPQVAWSVNMG
                     SLYNVLEVARRYRCSVFFPSSIGAFGPTTPRDRTPQVTIQRPTTIYGVTKVSGELLCD
                     YYARRFGVDARGLRLPGLISYSAPPGGGTTDYAVDIFHQAIRYRHYTCFLAGDTRLDM
                     MYMPDAIRAMIELMEADAGRLRFRNAYNVTAMSITPVELAEAIRKHIPDFVIEYRVDP
                     VRQAIAESWPRSLDDSAAREDWGWQPRFTLAAMVEDMLARLGERLQSREPPR"
     gene            complement(7725..8414)
                     /locus_tag="VF226_10960"
     CDS             complement(7725..8414)
                     /locus_tag="VF226_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX7074550.1"
                     /translation="MKSSNPFKDRRSAGKLLADRLKDLEPAHPVVLAMPRGGVPVAAE
                     VAKALHAPLDLVLVRKIGVPYQPELAAAAVVDGGEPEIVVNDEVVRMAGLTQDYIDEQ
                     AQEELAEIERRRQVYLEGRPRPSLENRTLILIDDGIATGASIRAAIAALKRKNPARLI
                     LAVPVAPADTIELLKTMVDDVVCLETPEPFFAIGMHYDDFHQVPDEEVVRIMNEVANV
                     EQEPARAGHSA"
     gene            complement(8411..9067)
                     /locus_tag="VF226_10965"
     CDS             complement(8411..9067)
                     /locus_tag="VF226_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX7074551.1"
                     /translation="MAQHQQARELTVEVGSHHLEGILRIPEDAIGLVIFAHGSGSSRV
                     SPRNTHVAHALNRRGLATLLFDLLTEQEAAYRANVFDIPLLGTRVAEAVRWARANAET
                     ADFRIGLFGSSTGAAAALVAAAQAPDDVSAIVSRGGRPDLAGNWLASVRAPTLLIVGG
                     HDFGVIELNEQALESLTCEKRLEIIPGATHLFEEPGTLDQVVELAGRWFETHLGSTLQ
                     "
     gene            9231..10949
                     /gene="polX"
                     /locus_tag="VF226_10970"
     CDS             9231..10949
                     /gene="polX"
                     /locus_tag="VF226_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX7074552.1"
                     /translation="MVVHNAEIAEMFNRLADLLEIEGANVFRVRAYRRAAQTIEDLPV
                     SAAEMLEKGEDLAKLPGIGRDLAGKIEEFVKTGHCSALDEVMARVPASLAALVAVPGL
                     GPKRVQLIHERLGVRSLEELAQAAREHKLRSLPRFSAKLEAQILSELEQHRTAERRFR
                     IATAADFAEALRRHIERCPGVERVVVAGSYRRCKETVGDLDILVTCAKSKPVIEHFVG
                     YEEVAQVAAKGSTRAAIALKAGLQADIRVVPSESYGAALHYFTGSKSHNIHIRKLGQS
                     RGLKINEYGVFRNGKRIGGRTEEEVFAAVGLPFIEPELREDRGEIEAAQAGRLPKLVE
                     QRDIRGDLHCHTNASDGKNSLVEMVEAARARGYEYLAITDHSKRAAVARGLDEKRLAK
                     QLDEIDRLNDKLDGFRVLKSSEVDILVDGKLDLPDRILKRLDIVVCSIHYKFDLDPKS
                     QTERILRAMDNRYFNILGHPTGRLLAERAGYALDLERVMRGAKERGCYLELNSHPSRL
                     DIDDVHCKLAKDLGVLVSIGTDAHSTMGLAVMRYGIGQARRGWLEAKDILNTRPWPAL
                     KALLKR"
     gene            10964..12070
                     /locus_tag="VF226_10975"
     CDS             10964..12070
                     /locus_tag="VF226_10975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX7074553.1"
                     /translation="MSSLKLMLTGDVMTGRGIDQILPHPCDPVLYEDYVKSALGYVAL
                     AEDASGPIPRRVPFTYVWGDSLGEMEKQKPDVRIINLETAITAGGAPEPKGINYRMHP
                     ANIGVLKAAGINACVLANNHVLDWGTVGLGETLETLSRAGITAVGAGRNEAEARAPLV
                     FGLPSGRRVVLFAYGSVDSGIPRSWGARGNTPGVNLLRDFSPRTVLQIGQDVRAVRQA
                     GDVVVASIHWGGNWGYEISDAHRDFAHALIDDAMVDIVHGHSSHHVKGIEIWHGKLVL
                     YGCGDLINDYEGISGYEEFRDDLSLIYFPKVKDGGGALESLEMAPFHMRAFRLEKADA
                     GDAAWLADLLTEQGRQFGTWAELAPDGNLRLGWS"
     gene            complement(12080..>12481)
                     /locus_tag="VF226_10980"
     CDS             complement(12080..>12481)
                     /locus_tag="VF226_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7074554.1"
                     /translation="PQVLWPDHCVQGTRGAELRDELDIPHAELILRKGYHRDIDSYSV
                     FFENDRTTPTGLAGYLRERGLSRAFFAGLALDFCVRYSVEDAHRISLDAVIVEDACRG
                     IGVDGSLESTREIISQLGIPLVTTEDISAGT"
BASE COUNT         2277 a         3906 c         4096 g         2202 t
ORIGIN      
        1 aaaaacactg ccgcttgctg caggaacgag ccatccatcg cggagatttc ccgacaagag
       61 attgcagaga taccgctcaa agcggccgac gcccgcaggt tccgtaaagc gcgcgacgtt
      121 tggcagtaag caggtccgcg cggggacgac gcgtgaaggt ggcggggcgg accggtatca
      181 gttgaagccc ctatcccctg cctctggctc aatccggcgg tgctacgatc tttccccgat
      241 tcatcgaggg ctgatcgtgg ctgctgctag tcgacgatga aaagcttggc gcctgtgcgg
      301 gtcgatgacc gatgagcggc atcgccgtgg tctgagacct gatagctcat gccgggcttc
      361 aatgtgaatg tgcgcccgtc gcggagctcg gtcttaagct cgccctccag aacgtacaga
      421 acgtgaccgc gatcgcacca atggtcggcg aggtagcctg gcgaatactc gacaatccgg
      481 acgcgcagat cgccaaccgt gaacgtgcgc cagcgggcct caccggtttc tcccggatgg
      541 acggtcggct cgatgctgtc ccaatccgtt acggtgaaag gcagcgtcgg aagcttcact
      601 gtgcccctcc tctggggttc agcattggag tatccggctc actctgtcaa agccacgatt
      661 ttcccaggat tcatgatgtt cagcggatcg agcgcacgct tgatctggcg catgatgtcg
      721 aggccggggc cgagctccga cttcaggtac tccatcttgc cttggccgat gccgtgctcc
      781 ccggtgcagg tgccgtccat cgcgatagcg cgctcggcaa ggcgcttgag gaagtcctcg
      841 atcttggccc gcgcggccgg atcattcatc gcgaacacgg gcgaggtatg gaagttgcca
      901 tcaccgacgt ggcctacgat cggcgcgatg atgccgtgct ccttgatgtc tttttgggtc
      961 tcgagcacgc attcggcgag ccgcgagatc ggcacgcaaa catccgtcgc aagcacgtcg
     1021 tggccgggcc aggtcgagcg gacggcccag taggcgtcgt ggcgggcctt ccagagccgg
     1081 cgacgatcct cctccttctc ggcccagtcg aaccggatgg cgccttccga cagcgcgatc
     1141 tccttgaact gctcgacctg atcgcgcgca gagacctccg tgccgtggaa ctcgaggaac
     1201 agcgtcgggc tctcctcgaa gctggttttg gagtgattgt tgaccgcgta gatctgcacc
     1261 tcgtcgagca gctccatgcg ggcgatgccg agcccgagct ggatcgattg gatgacggcg
     1321 ttgcaggcgc cctcgatagt cttgaacggg cacacggccg ctaggatcac ttccggaatg
     1381 ccatagaggc gcaccgtcag ctcggtgatg atgccgagcg taccctcgga gccgatcagg
     1441 agccgggtga gatcatagcc cgccgccgac ttccgggcgc gacgcgccgt gcgcacgacc
     1501 gagccgtctg ccatgatcgc ggtgaggttg agcacgttct cgcgcatggt gccgtagcgc
     1561 accgcgttgg tgcccgaggc gcgggtcgag gccatgccgc caagcgttgc ctcctcggca
     1621 cccgggtcta tggggaagaa cagacccatg tcgcgcagat gatcgttgat ctgcttgcgg
     1681 gagacgccgg cctggacgag gcagtcgaga tcacgatcgt tgacctgcag cacacggttc
     1741 atgcgggaaa ggtcgaccga gatgccgccg aaaggcgcat tcacgtgacc ttcgagcgag
     1801 gtgccggccc cgaacgggat cagcggcacg cgatggctgg ctgcaatgtg tacgatctca
     1861 cgcacctcgt cggtggactc gggccacacc acggcatccg gcggctgatt ggaaatccag
     1921 gtcagcgtgt gcccgtgctg gcggcggatc gcctcgcccg tctgcagccg gtcaccaaag
     1981 cgctgccgga gcactgcgat cgccgtctcg atcgtctcac gcgcaggctc cggaactgct
     2041 gctgtcgcca tctcgtctca tcccctctcg aaggctgcgg tgcagccatg gcgttccggg
     2101 cggatgctag cgtgacgaac gagacgttcg ccagtagttg cggcacgcaa cttcagcgca
     2161 atctcagtct gggccacgcc ggcaactcca aatcctcgtg cacctcttga cccaaagttc
     2221 gccccgaaac cggccgcgag ctctggcgaa cttcggagtc gggacactag tgggctaggg
     2281 gttgccatgc aaatgcgcca atcgtcgtcg aggtcgcagt cccggcaatt ctcctccgcg
     2341 tcccgcaggg acaacaggca ccgatttctg acctcacgcg tttgtaccgg ggcccccgca
     2401 atcagggcgc cttggcgaga gtcgggtcgc gtcggtcggc tttccgccat tctcgacaac
     2461 agcttcgaag aggttttcag atggtcggca ggtccggtat cgcgcttctc gccacgatcg
     2521 ccatttgcgg cggcgcggca ttcgcgcagc agacgacgcc cgtcccaccc ggtcagtctt
     2581 ccactggcca gcagcagacg agggtatcgc ccgagcaggc catccaaatg gcccgcaatc
     2641 aggggatggt ggacgtgcgt gagctcgatc gcgacggaaa tgtatgggag atcgaggggc
     2701 atgatgccca aggacaggag atcgaggtca aaatcaacat cctcacgggc gaggtcgtca
     2761 gcatagagcg aggctgacga aaggttgagc atttctgatc acaatacacc tgcgtctgtt
     2821 cacgcttgcg ccaaaccgga gcgccgctgc ctgacggttg tttcccagcc cagcttgcgc
     2881 tgtcataatc ggtgcgagag acctcgatcc gcggaggagc agagagatga agacgcgcgc
     2941 cgccatcgcg ttcgaaaagg ccaagccact cgaaatcgtc gagctcgacc tcgagggccc
     3001 gaaagaaggg gaggtcctgg tcgagatcaa ggcgacgggc atctgccaca cggacgactt
     3061 cacgctttcg ggtgccgatc cggaaggttt gttcccggcc gttctgggcc acgaaggcgc
     3121 tggcgtcgtg gtcgatgtcg gcccgggggt cacgagcgtc aagaagggtg atcacgtcat
     3181 tccgctctac acgcccgagt gccgcaactg cccctcctgc ctgtcgcgca agaccaactt
     3241 gtgcaccgcg atccgtgcga cgcaggggca gggcgtcatg cccgacggca ccagccggtt
     3301 ctcttatgaa ggcaggatgg ttcaccacta catgggcacc tccaccttct ccaacttcac
     3361 cgtgctgccg gaaatcgcgg tcgcgaaaat ccgcgaggac gcgcccttcg acaaggtctg
     3421 ctacatcggc tgcggcgtga ccaccggcat cggcgcggtc atcaatacgg cgaaggtgga
     3481 gccggggtcg actgcgatcg tgttcggtct cggcggcatc ggccttaacg tcatccaggg
     3541 cctgcggctc gtcggggccg acatgatcat cggcgtcgac atcaaccccg ataagaaggc
     3601 ctggggcgag cgcttcggca tgacgcactt cgtcaacccg aaggacgtgg gcggtgatct
     3661 cgtcgcctat ctcgtcaaca tgacgaagcg gggcgcggac cagatcggcg gtgccgacta
     3721 cacgttcgac tgcacgggca acgtcgacgt gatgcggacg gcgctcgagg cctgccatcg
     3781 gggctggggc cagtcgatca tcatcggcgt tgcgggggcc ggccaggaaa tccgcacgcg
     3841 cccattccag ctggtcacag gtcgcgtgtg gaagggcacg gccttcggcg gcgccagggg
     3901 ccgcacggac gttccgaaga tcgtcgattg gtacatggac ggcaagatcc agatcgaccc
     3961 gatgatcacc cacacgctgc cgctcgagcg tatcaacgaa ggcttcgacc tgatgcattc
     4021 gggcaagagc atccgcagcg tcgtcatcta ctgatgcgct cttgcttgga ggcgctgagc
     4081 ggttagagct ttttcttcag gtagaaacgc caacagcaag cactccgtta acctctcccg
     4141 tggggaaagg tcggcccgaa gggccgggtg agcatctgtg ttgaaagtca agcctgatgt
     4201 gcctgccaca gccgatcctc acggataagc acattggcaa gcacgacgag cttgcgcatg
     4261 acggcggtga tggcgacctt gggttttttg ccggccgcgc gcagccgccg gtagaacccg
     4321 gacaggccag gattgaagcg ggctgcggcg atggcggcga agtagagggc ggtgcgcaca
     4381 tgggcgcgcc cgcctcggat ccggcgttgg ccagcggtag cgccgctgtc gcaggcgagc
     4441 ggcgcgaggc cggccagaag accggccgcc ttgcccgagc aggtgccgag ctccggcaga
     4501 ccgatgatca acgccaccgc cgcgaccggg ccgatgccgg ggatcgagcg gacgatctca
     4561 tagcgccgct tgagggcagg atcggatgcg atacggcggt cgatctcggc ctcgaggcgg
     4621 gcgatatggg tggcgatggc cttcagccgg cggccgagct cggccttgag gaaggcggtc
     4681 tggctggccg cgcgctgatt ggtcaaagcc gtctggtccg cgatcgcggc cgcgcgtgcg
     4741 cgcaccagct cttgcagcgc cgcgagcgct tctgccacgg gcggggttgc tgccggctcg
     4801 agactttcgg caaggatcgc gagcacacgt gcatcgagcc gatcggtttt ggccagctgg
     4861 ccagtggctt cggcaaacag acgcgagcgc agcggattga caacggccac cgcgaacccc
     4921 gcggcgctga gggtgcggtg cggctggcgg tggtacttgg cggtggcctc catgacgatg
     4981 accacgacct ggagcccggc gagatgacgc ttgaggcgct tgaggccgtc cggcgtgctc
     5041 gcgatgcgga actggtggcc aatgggatgg agataaacgt cgagccagtc tttacagaca
     5101 tcgacgccga cgtagactcg cggtgcgctt tgctcgggcg gaaccttgcc ttgcatgcgg
     5161 gacttgctcc ctgtcatctg tccaggacaa gcgcaaagga cggacggacc aggctcaccc
     5221 gcggttctcg accttggggt ttacggtccc gcccgtccgg ccttcgggag gtggccacct
     5281 cccgaaggcc agcccgcatg ttcagtgatt cgggccgcaa tcaacatgca aggggttaca
     5341 acagccgcta ccggcggccc ccctcacccc gaccctctcc ccgccgggga gagggagatt
     5401 gggggcaagc ttgacttggc gtgcttctgt ctaaatcgga aatgctctag acctcagcgc
     5461 cccgcaaagg ctcccaaggc tgcgagagct tcgtcgacat cgaacggggt gtggtcggcg
     5521 ctgatctgga agcggatttc ctcgtcgccc ttgggcacga ccggaaagtt caagcccgtg
     5581 gccagaatgc cggcgttgcg caaatgggca acgagcgtgg ctgtccgcgt cgtatcgcga
     5641 accatcagcg gcacgaccgg gtgttcgccg ggcagcgttt cgaggccgag ggcggtcaaa
     5701 ccgtggcgaa agcgggccgt catctcacgg aggtgatcga gcagctgccg cccgcgcgga
     5761 ccgtcgacga tatcgaccgc cttgcaggcc gcagcggcct ccgccggcgt gatcgggttg
     5821 gagtagatgt agaacggcga cgtctcgcgc aggtaacgga tgaccgttgc gctgcccgtc
     5881 acgtagccac cgttgacgcc gaacgccttg ccgagcgtgc cgaccaggag atcggccttg
     5941 gctccagtca cctcctccgt acccctgccg gtgcggccga acgcgccgac gccatgggag
     6001 tcgtcgacca cgaggatcgc gttctccgcg aagttggcgt catgagcgtc gatgatgctg
     6061 cgcacggcat cgagcggcgc atgatcgccc cgcatgctga agacgccgtc ggtgacgacg
     6121 atggcgcggt cgcaagactt cgcggcttcc gtgaggtgac gctccagatc ggcgagatcg
     6181 agatgccgcc aaatgcgctt ctccgctggc cgcccgagtg caatcgcatt gatgatgcag
     6241 ttgtgattga gctcgtcgct aatgacggcg gtgcggtcgg tgatgagctg cgggagcagg
     6301 cccatgacgg tcgcgtaagc ggagctgaac agcatcgccg cctcgcgatc gtgaaaagca
     6361 gcaagccggc gctccagggc cacgtgcggc gcccacgtgc cgctgatgaa gcgaaccgcc
     6421 cccggcccgg tgccgtactt gaggaccgca ctgtcctcag ccgccttcac ctccgcgtcg
     6481 agcgacaggc cgagatagct gttggagttc atccgcagaa acggctgcgc gccatagcct
     6541 tcgagcagga agcgcggccc tttctggcca tcggccggaa tgacgccggt gatgacgctc
     6601 tccgcacctt tcagacgccc cgcgtggccg agctccgaca gccgctcggc gagcttcttc
     6661 tccaaccgtt ccgcgcccat cgcgacctcc cgtagctcat cgcggcggtt ctcgagattg
     6721 cagcctttca ccgagccgcg ccagcatatc ctcgaccatg gcagcgagcg tgaagcgagg
     6781 ctgccagccc cagtcctcgc gcgcggcact gtcgtccaag gaccgcggcc agctttccgc
     6841 gatcgcctgg cggacggggt cgaccctgta ttcgatgacg aaatccggga tgtgcttgcg
     6901 aatggcctcg gccagctcga ccggcgtgat gctcatggcc gtgacgttat aagcgttgcg
     6961 gaagcgcagc cggccggcat ctgcctccat cagctcgatc atcgcgcgga tcgcatccgg
     7021 catgtacatc atgtcaagcc gcgtatcgcc cgccagaaag cacgtgtaat gccggtatcg
     7081 gatcgcctga tgaaagatgt ccaccgcgta gtcggtcgtg ccgccaccgg gtggcgcgct
     7141 gtaggaaatg aggcccggca gacgcagacc ccgcgcatcg acgccgaagc ggcgggcata
     7201 gtagtcgcag agcagctcgc cggaaacctt ggtgacgccg taaatcgtcg tcggccgctg
     7261 gatcgtcacc tgcggcgtgc gatcacgcgg cgtcgtcggg ccgaaggcgc cgatcgagct
     7321 cgggaagaag acgctgcagc ggtagcgccg ggcaacctcg agcacattgt agaggctgcc
     7381 catgttgacg ctccaggcga cttggggctt cgcctccgcc gtcgccgaga ggatcgcagc
     7441 gagatgatag atggttccga catcatgccg ccggacgaca tcctggagct gcgcgagatc
     7501 cgtgcagtcc agttgctcgc tcggccctga aaagggctcg ccgagcggct ccatgcgcag
     7561 atcggaggat atgacacgct cgctgccgta gcggtcgcac aaggccggaa ccagttccga
     7621 tccgatctgt cccagcgcgc ccgtgacgag aattgatcgc gcgctcgacg acgctcgcgg
     7681 catgaggcca atccttcaaa gcccaagcgg aacggcgcgc catctcacgc gctgtgtcct
     7741 gcgcgtgcag gctcctgctc gacgttggcg acttcgttca tgatgcgaac gacctcctcg
     7801 tcagggacct gatgaaagtc gtcgtaatgc atgccaatgg cgaagaacgg ctccggcgtc
     7861 tcgaggcaga ccacgtcgtc gaccatcgtt ttcaagagct cgatcgtgtc ggccggcgcg
     7921 accggaacgg cgaggatcag ccgagcgggg ttcttccgtt tcagcgctgc aatggctgcc
     7981 cggatgctcg cacccgtggc gatgccgtcg tcgatcagga tgagggtacg gttctccagg
     8041 gagggccgcg gccttccctc gaggtatacc tgccgccgcc gctcgatttc ggcgagttcc
     8101 tcctgggcct gctcatcgat gtaatcctgg gtgaggccgg ccatccgcac cacctcgtca
     8161 ttgaccacga tctccggctc accgccgtca acgaccgccg ctgccgccag ctcgggctga
     8221 tagggcacgc cgattttgcg gacgaggacg aggtcgaggg gagcatgaag cgccttggcg
     8281 acctccgccg cgacaggcac gccgcctcgc ggcatggcga gcacgaccgg atgcgccggc
     8341 tcgaggtcct tcagccgatc ggcgagcagc ttgcccgcgc tcctgcggtc tttgaacgga
     8401 ttggacgact tcattggagc gtgctcccca ggtgcgtttc gaaccaccgg cccgcaagct
     8461 cgacaacctg atcaagcgtc ccgggctcct cgaacaggtg ggtcgcccct ggaatgattt
     8521 cgaggcgctt ctcacacgtg agcgattcga gcgcctgctc gttgagctcg atgacaccga
     8581 agtcatggcc gccgacgatg agcagcgtcg gcgcccggac gctcgccaac cagttgccgg
     8641 ccagatccgg gcggccaccg cgggatacaa tggcgctgac atcatccgga gcttgcgcag
     8701 cggcgacaag tgctgctgcg gcgccggtgc tgctgccgaa aaggccgatc cgaaagtccg
     8761 ccgtctccgc gttcgcgcgc gcccagcgga cggcctcggc gactcgcgtg ccgagcagag
     8821 gaatgtcgaa cacgttggcg cggtaggccg cctcttgctc ggtcagaagg tcgaagagca
     8881 gcgtggcgag gccgcgcctg ttgagcgcat gggcaacgtg tgtgttgcgg gggctgaccc
     8941 ggctcgatcc gctgccgtgg gcaaaaatga cgagtccgat cgcgtcctcg gggatgcgaa
     9001 ggatcccttc caaatgatga ctgccgacct cgacggtgag ctcgcgtgcc tgttggtgct
     9061 gggccatgac tgcctcgcgg ttcggttctg aggcaaccga ttgttctctg cgtgaccacg
     9121 catcgcatgc cccactttgg gccgggcggc agcggcgcgc attgatggtg gtcaacgttg
     9181 gaaccgctga aacgcgctca tcttttcaat ggacacgact gagccgcgcc gtggtcgttc
     9241 acaacgcaga gatcgccgaa atgttcaacc gcctcgccga tctgctcgag atcgaggggg
     9301 ccaacgtgtt tcgcgtgcgt gcctaccggc gggcggcgca gacaatcgag gacctgccag
     9361 tcagcgcggc cgaaatgctg gagaaaggtg aggaccttgc caagttgccc ggcatcggcc
     9421 gcgatctcgc tggcaagatc gaagaattcg tcaaaaccgg acattgctcc gcgctcgacg
     9481 aggtcatggc ccgagtacca gccagcctgg cagcgctggt cgccgtgccg gggctcgggc
     9541 cgaagcgcgt tcagctcatc cacgaacggc tcggagtgcg ctcgctggag gagctggccc
     9601 aggctgcgcg cgagcacaag ctgagatccc tgccgcggtt cagtgcgaag ctggaagccc
     9661 agatcctcag cgagctggag cagcacagga cggctgagcg ccgcttcagg attgccacag
     9721 cggccgattt cgctgaggcg ctgcgccggc acatcgagcg ctgccccggc gtcgagcggg
     9781 tcgtcgtggc gggtagctac aggcgatgca aggaaacggt aggcgatctc gacattctcg
     9841 tcacctgcgc aaaaagcaag ccggtcatcg agcatttcgt gggctacgag gaagtggccc
     9901 aggtggccgc caaaggctcg acgcgcgctg ccattgcgct caaggcgggc ctgcaggcgg
     9961 atatccgcgt ggtgccgtca gaaagctatg gagccgctct ccactacttc accggatcga
    10021 aatcgcacaa tatccatatc cgaaagctcg gtcagagccg cggtctcaag atcaacgaat
    10081 acggagtttt ccggaacggg aagcggattg gcgggcgcac ggaggaggag gtcttcgcgg
    10141 ccgttggtct gccattcatc gagccggagc tcagggagga tcgcggtgag atcgaggcgg
    10201 cgcaggctgg gcgcctgccg aagctggtcg agcaacgcga tattcgcggc gacctgcatt
    10261 gccacacgaa cgcatccgac ggaaagaaca gtctcgtcga gatggtcgag gctgcaaggg
    10321 cgcgcggcta cgagtatctc gcgatcaccg accattccaa acgcgcggcc gtggcccgcg
    10381 gcttggacga gaaacgcctg gcgaagcagc tcgacgaaat cgaccggctg aacgacaagc
    10441 tcgacgggtt cagagttctc aagtcctccg aagtcgacat tctggtggac ggaaagctcg
    10501 atttgcctga tcgcattctg aaaaggctcg atattgttgt ctgctcgatt cattacaaat
    10561 tcgatttgga ccccaaatcg caaaccgaac gcattttgcg ggcaatggat aatcgctact
    10621 tcaatattct ggggcatccg accggtcggc tgctcgccga gcgcgcgggc tacgccctcg
    10681 atcttgagcg ggtgatgcgc ggcgcaaaag aaaggggctg ctacctggag ctcaattcgc
    10741 acccatcgag gctcgatatc gacgatgtgc actgcaagct ggccaaggac cttggcgtcc
    10801 tcgtttccat cggcacggac gcgcactcga cgatggggct cgctgtcatg cgctacggca
    10861 tcggtcaggc gcgtcgaggc tggctcgagg caaaggacat cctcaatacg cgaccctggc
    10921 ccgccctgaa ggctctcctc aaacgctgac cccgggttca tcgatgtctt ccttaaagct
    10981 gatgctgaca ggcgatgtga tgaccggccg cggtattgac cagatcctgc cgcatccctg
    11041 cgacccggtc ctttacgagg actacgtcaa gagcgctctc ggctacgtgg ctcttgcgga
    11101 ggacgcttcc ggcccaattc cgaggcgcgt cccgttcaca tatgtctggg gcgactcgct
    11161 cggcgaaatg gaaaagcaga agccggatgt ccgcatcatc aatttggaga cggcgatcac
    11221 tgccggtggg gccccggaac ccaagggcat aaactaccgg atgcaccctg cgaacatcgg
    11281 cgtactgaag gccgctggaa tcaacgcttg cgtattggcg aacaatcacg tcctggattg
    11341 gggcacggtg gggcttggcg aaacgctgga aacgctgagc agggcgggta ttaccgccgt
    11401 cggtgccggc cggaatgagg ccgaggcgcg tgcgcccctt gttttcggct tgccctcggg
    11461 gcgccgtgtc gtccttttcg cctatggctc cgtggacagc ggtatccccc gaagctgggg
    11521 cgcgcgcggg aacacgcccg gtgtcaatct gctgcgggat ttttcaccga gaacggtgct
    11581 ccagatcggg caagacgtgc gggctgtcag gcaagcgggc gatgtcgtgg tcgcctcgat
    11641 tcattgggga ggcaactggg gctatgagat cagcgatgcg caccgcgatt tcgcgcacgc
    11701 gctgatcgac gatgccatgg tcgatatcgt gcacggccat tcctcgcacc acgtgaaggg
    11761 catcgaaatc tggcacggca agctcgttct ctacggttgt ggcgacctta tcaatgacta
    11821 cgagggcatc agcggatacg aggagttccg cgatgatctg tccctgattt actttccgaa
    11881 ggtaaaggac ggcggaggcg cgctggagtc tttggaaatg gctccgtttc acatgcgcgc
    11941 atttcgactg gagaaagccg atgccggcga tgccgcctgg cttgccgatc tcttgacgga
    12001 gcaggggcgc cagttcggga cctgggcgga actggcgccc gacggcaact tgcggctcgg
    12061 gtggagctga gcctcaggcc tatgtgccag ccgagatatc ctccgtcgtg acgagcggaa
    12121 ttccgagctg ggagatgatt tcgcgcgtgc tctccaatga gccatcaacc ccaatgcctc
    12181 ggcaggcgtc ttcgacgatc acggcgtcaa ggctgatgcg atgggcgtcc tcgaccgagt
    12241 accggacgca gaagtcgagc gccaggcccg cgaagaacgc gcgtgagagg ccccgctccc
    12301 gcagatagcc ggcgagcccc gttggcgtcg tccggtcgtt ctcgaagaaa acggaatagg
    12361 agtcgatgtc tcgatgatag cccttcctca gaatcagctc ggcatgcgga atatcgagtt
    12421 cgtcgcgcag ctcggccccg cgcgtgccct gaacacagtg atccggccac aagacctggg
    12481 g
//
LOCUS       DASVDU010000174         8254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_106495, whole genome shotgun sequence.
ACCESSION   DASVDU010000174 DASVDU010000000
VERSION     DASVDU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106495"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="VF226_10985"
     CDS             <1..1862
                     /locus_tag="VF226_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074555.1"
                     /translation="EIPEALWLDANLEGEESRVTLFRPIAEEKVREVFSAAAFNYAFH
                     SRISRRAEATDADVEAALDVARRFVSARMTPPKEDGTQAEWDRYIAWSGAVVQALKIL
                     ASTGTADDAALIAAAVESKETVGADRSLMHLAYLEQRLGLLANGRVAAYADGQDPNIA
                     AYALVLLDYYGVAGAKERLFALAADGKRWGNLQPLARERIAQRLSGLDDPRVGEVASA
                     ALRRALEHIPAFETKRYQLFLHELTRSFFSYLAVFGSTEEKRTIVEVFDKRGYWKGWL
                     AQPLAMIAIDPLPFIEEFIEEGISKKIIDTSSPAACALWRLRDEAEFAALHEMLIDAY
                     ARYRRDAVHKKKTSSSLRERETYAIQASTARVLFAPCRPDKVAADVYYKDKVNSRTVF
                     PGNWFPAFRTVDALREVMTEQPYERAPLEAAPVEIGLAAWQQLEHVRWSSFRDERMAM
                     FRAFTNSGDDEVNGVVDDGIERRVYVLRHVVKDSYSGAINGLLEVRPMAEDGKLVIGV
                     RLRQAAWYNSFGGIADMIATGGDRNQWAHHAYVVDGGRKLIEHIALHRDGTVVPLVEE
                     GRDAEGFFRFATDLSVDDLSRLVLEIRLALLEDRRSFQFDVFASKLARNRPVQ"
     gene            complement(2244..2540)
                     /locus_tag="VF226_10990"
     CDS             complement(2244..2540)
                     /locus_tag="VF226_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074556.1"
                     /translation="MRLPPFGEPHQPLDIRIVGRNNPALERIELLLPCPINLLQAGNP
                     GLAVIPLAYGFLDLPSLTRDVIQLTFQACRIVPRTHNRSGRFAGWCIAIQLLTP"
     gene            2511..4010
                     /locus_tag="VF226_10995"
     CDS             2511..4010
                     /locus_tag="VF226_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074557.1"
                     /translation="MRLAERWKAHGRACANMECPELECAGARRHLQALFDAEAALEDM
                     VRQLHAARDDARRHFHALHEQLARSQARQDLLEKSLAAQQLLYDAGSALLEIADIHSA
                     LSKLADYDQFMDASWFDRADALYNIVRNTESSLTTVINRLSDSEHTAAFATIEKGIGK
                     TIRFDPKIVDGIGGAKDTLVNLKDILEEVKKWKEGAAARAIQPEEMFTLWQTTGRAAQ
                     IRGSLMQIAARPLKAWAGMEIEKVKDELRELARIIAAEDSVIGPAAEYMWRINGRRYA
                     AEDALEALREARQAAENCVMRGCKGAQTLQPARLAPSSGWGEGLRDHNKRLPGFVAAL
                     SGGGEAPSGRICTGQPVTLVLRQSGKKPPRGRSIVEAIENVGKAIETADKPDWVIEYT
                     ITARPRFERRPDGSVVARFDAVEMNIKDDPGMPDDMRQAGRREAAAVRARLGRAAVGV
                     DAAAVAKGEYSVDLRSAVGALSTLSAIGESARAFCRRPDPANVCQPAGN"
     gene            4065..4958
                     /locus_tag="VF226_11000"
     CDS             4065..4958
                     /locus_tag="VF226_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074558.1"
                     /translation="MKKLTHVERICCLPAPPPGWHDYDYEVLDDSRLVLVRVDSDVAS
                     VSPHDRARVWPKARLRLSCFDGATESDLLELAAVPWPKVCKLPDGRWLVVQSRCSWSA
                     RNVSIFNPDGSRAENFAVGDGVGPIVCLPDGRLWVGYIDQGVFGDPRRKHPASSGLAA
                     FDIGGACVGRFSHQEYAICDCYALGATGSVVWACTYTDFPIIRIDDGGVRLWHNSIDG
                     AFAIAANQTHVMLAGGYHEDANRLMLLRLEGRNAVPVDEFRLATDDGKIAWAGRNGIM
                     HATVGREWLQIRIDDWLATVW"
     gene            5409..7355
                     /locus_tag="VF226_11005"
     CDS             5409..7355
                     /locus_tag="VF226_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX7074559.1"
                     /translation="MVAQPSTDYPSVCLCLLSVGTIVRARETAQTRVGEGNGQRAITA
                     WARFRRQVGKLQLRRADHTRGRIVMNLFEDTNPRALKDLLSEIHNRTSVLPDFQRDFV
                     WEPGATQELIVSIANNYPAGSILRVRDSKRVFAAREFEGAPRLDGCQHTFLVLDGQQR
                     LTSLYQAFYGAGEHRYFLDLRKLIDGVDFEEAIFHVRAATKWAKQREDFDLQAKELLL
                     PLSVLKGGAGRFGQWSRKVCRRLPHAERIDLEDKLAEIEETWIRVIDDYHFPVVTLSD
                     KTEPDALCTIFETLNRTGVKLSVFELLTARFWPQNVNLRAMWDAALAQNAILADFDVD
                     PYYVLQAISLASRKAPSCKRGDVLNLTAEDIKEWWDMVIRGLVLGLEILRDDCKVMLP
                     KWLPYKTMMPPLAAVIAKVGTPRSAQAGVHREKLKRWFWCAVFGQSYESAPNSKSAKD
                     VTELSEWLMGGDPPESVTSFRFDPRALRDVTPRQRSIYRGAICLMLCGGARDFQTQAV
                     ISGRLMNEQGIDDHHIFPAAYLERKGVALSRLRDCVLNRTLIDRTTNQIISDRAPSDY
                     LEEIRALPDFPFEAVLSSHGLPADESSPLFRNDYETFLSQRQDWLWAKIKEVTKADEA
                     ADLEADERGGRMSTRKKLLTSPCY"
     gene            7406..>8254
                     /locus_tag="VF226_11010"
     CDS             7406..>8254
                     /locus_tag="VF226_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7074560.1"
                     /translation="MRIRAAVLEEMGRKPPYATSRPLTISEVDLAPPGPGEVLVKIAA
                     AGLCHSDLSVIDGSRPRPTPMALGHEASAIVEETGPGVDDLVKGDHAVLVFVPSCGHC
                     APCATGRPALCEPAAAANGAGTLLSGARRLSRNGAPLHHHLGVSAFAEYATVSRRSLV
                     KIDKDVPLDLAALFGCAVLTGVGAVANTAKVEPGSTVAVIGLGGVGLAALLGAIAVGA
                     ERIIAVDLSPDKLDLAKTLGATDVFNAADSDVADSIREATKGGVDVAAELAGSVQALT
                     LAYRITR"
BASE COUNT         1640 a         2385 c         2625 g         1604 t
ORIGIN      
        1 tcgagatacc ggaagcgctc tggctcgatg ccaatctcga aggcgaggaa agccgcgtca
       61 cgcttttccg gcccatagcg gaggaaaagg tgcgcgaggt tttcagcgcc gcggcgttca
      121 actacgcatt tcattcgcga atttctcgcc gtgctgaagc gacggatgcc gacgtcgagg
      181 ctgcccttga tgttgcgcga agatttgttt ccgcaagaat gacccctcca aaggaggatg
      241 gcacccaagc ggaatgggat cgctatatcg cttggagtgg cgctgttgtg caggctttga
      301 aaatactggc cagcaccggc accgccgatg acgctgcatt gattgccgcg gctgtcgagt
      361 ccaaagaaac tgttggtgcc gacagatctc tgatgcacct ggcctatttg gagcaaaggt
      421 tggggttgct agccaatggc cgcgttgccg cctatgcgga tgggcaggac cctaatattg
      481 cggcttacgc actggtgctc ctcgactatt acggcgtggc gggcgcgaaa gagcgcctgt
      541 ttgccctcgc tgccgacgga aagcggtggg gtaatcttca gccactggca agggagagaa
      601 tcgcgcagcg cctgagcggg ttggacgatc cgcgcgttgg cgaagtcgcg agtgcagcct
      661 tgcggcgcgc gttggagcat atccctgctt tcgaaaccaa acgttaccaa ctgtttttac
      721 atgaattgac aaggtccttt ttcagttatt tagcagtgtt tggatcaacc gaggaaaaac
      781 gcacgatagt cgaggttttc gacaagaggg gttattggaa aggatggctc gcccaaccgc
      841 tcgcaatgat cgccatcgac ccactgcctt tcattgagga gttcatcgaa gaaggaatta
      901 gcaagaagat catcgacacg agtagtccgg ctgcttgtgc gctctggcga ttgcgcgacg
      961 aggccgagtt cgcagcgctt catgaaatgt tgatagatgc ttatgcgcga tatcggcgcg
     1021 atgccgtgca caagaagaag accagctcat cgctcaggga gcgggaaaca tacgctatcc
     1081 aggcaagcac ggcccgtgtc ctattcgcgc cgtgccgccc ggataaggtc gccgctgatg
     1141 tctactacaa ggacaaagtg aattcccgca cggtatttcc cgggaattgg tttcccgcgt
     1201 tcaggacggt cgacgccctg cgcgaggtga tgaccgagca accatatgag cgcgcgcctc
     1261 tcgaggctgc accggtggag atcgggctcg ctgcctggca acagctcgag cacgtgcgct
     1321 ggtcatcttt ccgcgacgag cgcatggcga tgttccgcgc gttcaccaac tctggcgacg
     1381 acgaggtgaa cggcgttgtg gacgacggca ttgaacggcg tgtctatgtg ctgcgccacg
     1441 tcgtgaagga ctcgtattca ggtgcgatca atggacttct ggaagtgcgg ccgatggccg
     1501 aagacggcaa gctcgtgatt ggcgtgcggc tgcgtcaggc ggcgtggtac aacagctttg
     1561 gcggcatcgc cgacatgatc gctaccggtg gtgaccgcaa tcagtgggcg catcatgcct
     1621 atgtcgtgga tggcgggcgc aagttgatcg agcacatcgc gcttcaccgc gatggcacgg
     1681 tggtgccgct cgtggaagaa ggccgcgacg ctgaaggctt tttccggttc gccacagatc
     1741 tttccgtcga cgatctctcc cggcttgttc tcgaaatccg gctcgcgctg ttagaggatc
     1801 gccgctcgtt ccagtttgac gtgtttgcga gcaagctcgc acgcaacagg ccagtgcagt
     1861 aggagatcgc catgtggccc gcatcgatcc atccttaccg ctttcgaatg agcggccggc
     1921 ttggccttac ccttgccctc gcgatgattt tcattcagcc ggccactgcc gactggcaaa
     1981 gtgactaccg aagggcgcgg ctccagcgcg acatcgccgg ctgggacctg gagcgcgcca
     2041 aggcctgtgt ggcgagagcg tcggcgcggg tggcggcgtt gcagcggcgc gtggattttg
     2101 cggagctgga gacgtggaga cgccaatatg agctcgcgcg ccagaaacag caggagctgg
     2161 aggctgccgt cagcttctat cgcgccaaca gggacaaaga tcccaatgcg cagccttgga
     2221 gcaggagcca gctatggctt tggctaaggg gtgaggagct ggatcgcaat acaccacccg
     2281 gcgaaacgcc cactgcgatt gtgagtgcgc gggacgatgc gacacgcttg aaaggtaagc
     2341 tggatcacgt cgcgcgtgag cgaggggaga tcgagaaagc catacgcgag cgggatgact
     2401 gccaatcccg gattcccggc ctggaggagg tttatcgggc aaggcaggag cagctcgatg
     2461 cgttcgagcg cgggattatt cctcccgacg atgcggatat cgagcggctg atgcggctcg
     2521 ccgaacggtg gaaggcgcat ggccgggcgt gcgccaacat ggaatgcccc gagctcgaat
     2581 gtgcgggcgc ccgccgccat ttgcaggcgc tgttcgacgc cgaagcagcc ctggaggata
     2641 tggtgcgaca gttgcacgcc gcgcgcgacg atgcacgccg gcattttcac gcgctgcacg
     2701 agcagctcgc ccgcagccaa gcccgtcagg atctcttgga aaaatcactg gccgcgcaac
     2761 aactcctgta cgacgctgga agcgcgcttc tcgaaatcgc ggacatccac agcgcgctaa
     2821 gcaagttggc ggattatgat caattcatgg acgccagctg gttcgaccgg gctgatgctc
     2881 tctacaacat cgtgaggaac accgagagct cgttgaccac cgtcatcaac agactgtccg
     2941 acagcgaaca tacggcggct ttcgccacaa tcgaaaaagg cataggcaaa acaattcggt
     3001 ttgacccgaa gatcgtagac ggaattggtg gcgcgaagga taccttggtc aacctgaagg
     3061 acatcctgga agaagtaaaa aaatggaaag aaggtgctgc agcgcgtgcg atccagcccg
     3121 aagagatgtt cactctttgg cagacgacgg ggagggcggc gcaaatccgc ggcagtctca
     3181 tgcagatcgc cgcgcgcccc ctcaaggcgt gggccggcat ggaaatcgag aaggtgaagg
     3241 atgagctgcg tgagcttgcg cggatcatcg cggctgaaga ctcggtaatc ggtccggcag
     3301 cggagtacat gtggcgcatc aacggccgcc gctacgccgc agaggatgct ctcgaggctt
     3361 tacgcgaggc tcggcaggca gcagagaact gcgtcatgcg cggctgcaag ggagctcaaa
     3421 cgctacagcc ggccagattg gcgccgtctt caggatgggg cgaggggctg cgtgatcaca
     3481 acaagcgttt gcccggcttt gtcgctgccc tgagtggcgg cggggaggcg ccgtccggcc
     3541 gcatctgcac gggtcaaccg gtgacgctgg ttttgcggca gagcggcaag aagcctccac
     3601 gtggccgctc gattgtcgag gcgatcgaaa acgtcggcaa ggccatcgag accgccgata
     3661 agccagactg ggtcatagaa tatactataa ccgcgcgtcc gcgtttcgaa cggcgaccgg
     3721 acggatcggt ggtcgcccgc ttcgatgcag ttgagatgaa cattaaggac gatcccggta
     3781 tgccggacga catgcgccaa gctggccgtc gcgaggctgc ggcggttaga gcccgcctcg
     3841 gccgcgccgc ggtcggcgtg gacgccgccg ccgtcgcaaa gggcgaatat tcggtcgact
     3901 tacgttcggc ggtgggagcg ctgtccaccc tgtccgctat tggcgagagc gcccgagcct
     3961 tctgtcggag acccgatccc gccaacgtct gtcagcccgc cggaaattga aaggactgag
     4021 tcttctccac gtgaggctcg gcggacatac ttcggggtct agctatgaaa aagctgacgc
     4081 acgtcgagcg gatctgctgt ctaccggcac cgccaccggg ctggcacgac tacgactatg
     4141 aggtgcttga cgacagcagg cttgttttgg ttcgggtgga cagcgacgta gcttcggttt
     4201 caccgcatga ccgtgcgcga gtctggccaa aggcgcggct ccgtctttcc tgttttgatg
     4261 gtgcgaccga gagtgacctc ctggagcttg ccgcagttcc ctggccgaag gtctgcaagc
     4321 ttccggatgg aagatggctg gtcgtgcaat cacgctgcag ctggagcgcg cgcaatgttt
     4381 cgatcttcaa tccggatggt tcccgggcgg aaaactttgc ggtcggcgat ggtgtcgggc
     4441 ccattgtgtg cctgccggac ggcaggcttt gggtcggata catcgaccaa ggcgtattcg
     4501 gggatccccg ccgcaagcac cctgcgagct caggcctagc cgcgttcgat attggtgggg
     4561 cgtgcgtagg gcgtttctcg catcaggaat atgcgatttg cgactgctac gcgctgggcg
     4621 cgactggatc ggtcgtctgg gcctgcacgt acaccgactt tcccattatt cggatcgacg
     4681 acggcggcgt gcggctttgg cataatagca tagatggcgc gttcgccatt gccgccaatc
     4741 aaacccacgt catgctcgcg ggcggatacc acgaggatgc gaaccgactg atgctcctgc
     4801 ggctggaagg aagaaacgct gtgcccgtcg acgaattccg cctcgcaacc gacgatggga
     4861 agatcgcatg ggcggggcgc aatggcatca tgcacgcgac agtcggccgc gaatggcttc
     4921 aaatcaggat cgacgactgg cttgcgaccg tttggtgagc cgtaccgggg cgcaaaccga
     4981 taattgacct cggcttaatg gccgacgccc ctgccgccgc gcgtggtcga gatcgacgcc
     5041 atgcagcgac cgtgctgcag cgcccattcg gaatcggcgg ggaatgtcct agtctgcgaa
     5101 ggcgaaaaca tcaggatgat gcccctgact gaccgcaaag cactgctgtc tgcgctcatt
     5161 gagcacgcgc agcttccttg cctgagctag gcagctggct ttgtggttca tggacaagct
     5221 cggcctcgag tgcgtgatct cgaagactat cgaatctcga tacgacaccg tggactctgt
     5281 actgaaggcg cggggcggga tgatcccagc aacgtgacgg atcggcgttg cggagggaaa
     5341 gagtcggccc agccgaggaa ctctgtggcc gggccaaccc cggtggatgc aatttggaac
     5401 agtggtgagt ggttgctcaa ccctccactg attatccatc agtttgtttg tgcctgttat
     5461 cagttgggac aattgtgcgc gccagagaaa ctgcgcaaac gagggtaggc gaggggaatg
     5521 gccaaagggc cattacagcc tgggccagat ttcggcgcca agtcggcaaa ttgcagcttc
     5581 gccgagctga tcacacgcgg gggagaattg tcatgaacct tttcgaggac accaatccac
     5641 gagctctaaa agacttactc agtgagattc acaatcgcac gtctgttctc ccggattttc
     5701 agcgcgattt cgtctgggag ccgggggcga ctcaggagct gattgtatca atcgcgaaca
     5761 actacccggc aggtagcatc ttgcgcgttc gtgattccaa gcgcgttttt gcagcccgcg
     5821 agttcgaagg cgccccgcgg cttgatggct gccaacacac gtttttggtg ctcgatggac
     5881 agcaacggct gacctcgctt tatcaagcct tctatggtgc cggagagcac agatatttcc
     5941 tcgatcttcg caagctcatc gatggcgtcg attttgaaga ggcaatcttc cacgtgcgcg
     6001 cagcaaccaa gtgggcaaaa cagcgcgaag acttcgatct ccaggcgaag gaattacttt
     6061 tgccgctctc cgtcctcaaa ggaggtgctg gccggtttgg ccagtggagc cgcaaagttt
     6121 gccgccggct gccccatgcg gagagaatag acctcgagga caaattggcc gaaatcgagg
     6181 agacttggat ccgcgtcatc gatgactacc atttccccgt cgtcacgctg tccgacaaga
     6241 ccgagcccga tgccctttgc acaatatttg agacgctcaa ccgaaccggc gtcaagctca
     6301 gcgtatttga gcttctgacg gcgcgctttt ggccgcaaaa cgtgaatttg cgggcgatgt
     6361 gggacgccgc cttggctcag aacgcgatcc tggccgactt cgatgttgat ccgtactatg
     6421 tcttgcaggc gatttcgctc gccagccgca aggctccttc atgcaagcgg ggcgatgtat
     6481 tgaacctgac cgccgaggac atcaaagagt ggtgggacat ggtcatccgg ggcctcgttc
     6541 tggggctcga gatcctgcgg gacgactgca aggtcatgct gcccaagtgg ctgccttata
     6601 agaccatgat gccgcccctc gccgccgtga ttgcaaaagt gggcacgccg agatccgcac
     6661 aggccggcgt gcatcgtgag aaactgaagc ggtggttctg gtgtgcggtg ttcggtcaat
     6721 cctatgagag tgcgcctaac agcaaatcgg ccaaggacgt cactgagctc tctgaatggc
     6781 ttatgggcgg cgacccaccg gaatctgtca cctcattccg tttcgatcct cgcgccctcc
     6841 gtgatgtcac gccgcgccag cgatcgatct atcgcggagc catctgcctc atgctttgcg
     6901 gaggcgccag agacttccag acgcaggccg tcataagcgg acgccttatg aacgagcagg
     6961 gcatcgacga tcaccacatc ttccctgccg catatctcga gcgaaaagga gtagcactgt
     7021 ctcgccttcg cgactgtgtg ctcaatcgaa cactcatcga ccggacgacg aaccagatca
     7081 tcagcgaccg cgctccttcg gattacctgg aagagatccg tgccctcccc gacttcccat
     7141 tcgaagcggt tctcagttcg cacgggcttc cggctgacga gtcctcgccg ttgttccgca
     7201 acgactacga gaccttcttg agtcagcgcc aggattggct ctgggcgaaa atcaaggagg
     7261 tcacaaaagc cgacgaagca gcggatcttg aagccgacga gagggggggc cgcatgagca
     7321 cgcgcaagaa actcttgacg tcgccctgct actgaaggcc attaacaagc aacgccgccg
     7381 cggcctaaac acgaggatga ctgccatgcg aatccgcgct gcagtgcttg aggaaatggg
     7441 gcggaagccg ccctatgcga cctcccgccc gctcaccatt tcggaggtgg atctggcgcc
     7501 gcccggccct ggtgaggtcc tcgtcaaaat cgcagccgct ggcctgtgcc attcggatct
     7561 ctcggtgatc gatggctcga ggccgcgccc cacgccgatg gcactcggtc acgaggcttc
     7621 tgccatcgtg gaggagacgg gccccggcgt cgatgatctc gtcaagggcg atcacgcggt
     7681 gctcgtgttc gttccgagct gcggtcattg tgcaccctgc gccactggac ggccggcgct
     7741 gtgtgagccg gcggcggcgg ccaacggcgc cggcacgctg ctgtcgggag cgcggcggct
     7801 ttcgcggaac ggtgcgccgc ttcatcatca cctcggcgtg tcggcctttg ccgagtatgc
     7861 gacggtgtcg cgccgctccc tggtcaagat cgacaaggat gtcccgctcg acctggcagc
     7921 actgtttgga tgcgccgtgc ttactggcgt cggtgccgtc gccaacacgg cgaaggtgga
     7981 gccgggatcg accgttgcgg tcatcggcct cggcggcgtt ggcctcgccg cactgctcgg
     8041 cgcgatcgcg gtcggcgcgg agcgcatcat cgcggtggat ctctcgcccg acaagctgga
     8101 cctcgccaag acgctcggtg ccactgacgt tttcaatgcc gcggattctg acgtggcaga
     8161 cagcatccgc gaggcaacga agggcggtgt tgacgtggct gcggagctgg caggctccgt
     8221 tcaggcgctg acgctcgcct accgcatcac gcgc
//
LOCUS       DASVDU010000175        14823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_402232, whole genome shotgun sequence.
ACCESSION   DASVDU010000175 DASVDU010000000
VERSION     DASVDU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402232"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VF226_11015"
     CDS             <1..645
                     /locus_tag="VF226_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074561.1"
                     /translation="VLLGGVPGVDPAKVVVLGGGVVGTNAVHIALGMGADVWVIDRNV
                     DVLRRLWSHFGRPLNTVYSTRDAIDRHLRDADLVIGAVLIPGAETPKIITREHVKMMR
                     PGSVIVDVAIDQGGCAETSRPTTHDNPTYTVDGVIHYCVANMPGGVPKTSTQALNNAT
                     LPFVIALAEKGWKRALAEDEHLRHGLNIWDGKVTCKPVADAQGYPYLPAEEALG"
     gene            818..1585
                     /locus_tag="VF226_11020"
     CDS             818..1585
                     /locus_tag="VF226_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074562.1"
                     /translation="MLEVRDLVKNFGGLRAIDHCSLSVREGTITGLIGPNGAGKTTLF
                     NLVTGFFQPDSGRVLADGKDITSLPPHGVFRRGICRTFQIPREHGTMTVLENLMLVAP
                     DQAGERFWNCWFRSGLVRQQEREIRDRAMEVLEFVDLARLANEYAGRLSGGQKKLLEL
                     ARTLMANPRLVLLDEPAAGVNRTLMKKLVDNIEYLRRERGITFLLIEHDMDLVMELCD
                     PVIVMSEGRRLMEGRPEEVRRDPRVLEAYLGGQYGAA"
     gene            1572..2279
                     /locus_tag="VF226_11025"
     CDS             1572..2279
                     /locus_tag="VF226_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074563.1"
                     /translation="MALLEVRGVTAGYGDTEILRNVSMEVGEGEIVSIIGPNGAGKST
                     LMKTIFGLLHPREGSIIFDGQDISRLQPYQIVERGMCYVPQVANVFTDLTVSENLEMG
                     AFLAKTNDIAARKERIYEYFPRLKERRNQRAGKMSGGERQMVAMGSALMLEPKLVLMD
                     EPSAGLSPKMVGVIFEQIRKINEAGPAILIVEQNAKLSLEMSHRGYVLASGENRFEGT
                     GEELLNDPEVGRLYLGG"
     gene            2306..3370
                     /locus_tag="VF226_11030"
     CDS             2306..3370
                     /locus_tag="VF226_11030"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7074564.1"
                     /translation="MAEILQEAPGRSDDRTTHERIMAALRSRPALGVALFLTLVVFWL
                     LGQQQGASLSDLLSLTVWGVMLGGIIALGAIGLTLVYGVLKFPNFAHGELVTLGAYGA
                     LTISALLPAGPAMQPFSFGWELMAGLILSMPIVSLIALVVDRVLFRPLRLRFASLVLF
                     AMASLAAAFFLRSAIYLVWGSDFLFYYPGRANPAIEMPLGIRVQADQLFVFGLAVVLV
                     IITWFLLERTRMGKAMRATANNPDLAQVRGINTERVIAWTWILGASLAAAGGVMYGLA
                     SQLRPEMGFWLLLPMFAAAIVGGLGSPIGAIAGAVIIGVAQQVSSSFLNPAYGPGVAF
                     ILLVIVLVLQPQGLFGQGGD"
     gene            3372..4559
                     /locus_tag="VF226_11035"
     CDS             3372..4559
                     /locus_tag="VF226_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7074565.1"
                     /translation="MAKADLARAAFLFLPLVLLGLLVLPGRMRLRLPLSLVIAAVVAL
                     ALPMLLPRGALTYLASFSTMAGIYAILCLGLNSQWGLTGHVNFGIAGFFAIGAFTSAL
                     VTTNMPQGALALYSQQAFGLGWPIIAGILTAGVVSAVAGFLISIPVLRLRTDFLAIAT
                     LGIAEVIRFIFQNERWLANGPQPMRGIPRPFDCVFTDPPCAWLPEGVARFVAPLAPRD
                     YVYLYLVIVAIILAVIFVLLERISRSPWGRLQRAIRDEENSAAMSGKNVPLRRVESFV
                     LGSFIMGVAGAVYAHYVVSIDYSHFNPLFATFIVWVMVMLGGSGNNKGAVLGAFAVWG
                     VWSGAAFMIDVMSPALQAISPDLPSRGAYLRWMLVAVMLGLIVLFRPQGILEERKKVS
                     DFM"
     gene            complement(4825..6171)
                     /locus_tag="VF226_11040"
     CDS             complement(4825..6171)
                     /locus_tag="VF226_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074566.1"
                     /translation="MSDFSLRNARRRDFLKAVGAAGGAMTLSVTMPRALWAQSAPPKV
                     PIGQVVAFTGSGAEYGPFFRDAAQLALNQINTAAEEVFGGPIVERLIAEDSNTLPTPA
                     IEAARKLVEADGVPAIICEWSSGVTVAVATSVTIPSGVLQVGNGTTSPLVSVLPEDEG
                     KDLLFRTSPSDALQGVVAADLAAGKLIDGYKFETAATIYINNPYGQGLSNAFAAAFEK
                     RGGKVLAQVAHAEEVQPTYRSILSTALQGKPGVLVLPTYPAHTAQILKESRDIFGYTS
                     WQFTDANRSSEVLDAVGGKDLAGKLGTVPSADPESQSYKTFAEAYRAATKQDRIPPFS
                     ETTYDAAMVIGLALAKAVADGIKDPAEITGTALRDRLRPIANPPGEKIIGGSQEQITK
                     ALQLLKEGKEIDYVGAGGSVDFDQNGDVKTPIAVWKFTETGDETVKIVPADQIASE"
     gene            complement(6483..6689)
                     /locus_tag="VF226_11045"
     CDS             complement(6483..6689)
                     /locus_tag="VF226_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074567.1"
                     /translation="MGNRGEKLPSECVELFAELDDFEDLRDAYARVQDRIRSYSAAGR
                     PVPEALRRCERHLMTEFMAQSQGR"
     gene            7073..8179
                     /locus_tag="VF226_11050"
     CDS             7073..8179
                     /locus_tag="VF226_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7074568.1"
                     /translation="MTIRRRQFLAGVATAGAAAGATALAKPAIAQGKTEWRMVTTWPK
                     NFPGQGTGAERFAKTVTEATDGRLTIKVYAAGEIVPAFESIDAVIRGAADLMHATPYY
                     WPNKSKALNFFSTVPFGMTTPELDAWMNHLGGQQLWDKLYDQFGLKPFHAGSTGVQMG
                     GWFNKEITGVDSVKGMKMRIPGIGGEALGKLGMTIVNLPAGEIFAALQSGAIDATEWV
                     GPYNDLALGFYKVAKYYYWPGMHEPGTQIEVTVDKAKFTALPKDVQRIFEACCHNENN
                     LLTSEFNARNGGALETLIRQHNVQLKRFPNDFLQAYGNAAGEVIQEMRDSGDPLTKEI
                     VESFLKARRDLMNWGRIADQGFMNARLLDIKFPT"
     gene            complement(8245..8454)
                     /locus_tag="VF226_11055"
     CDS             complement(8245..8454)
                     /locus_tag="VF226_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074569.1"
                     /translation="MEDLTSIEIGAGLLVLWFLTLFALWRLIDRQNRPGPIKSRLAKE
                     CLMLVHMTVLVVGIAMVLTGLHVVG"
     gene            complement(8469..9992)
                     /locus_tag="VF226_11060"
     CDS             complement(8469..9992)
                     /locus_tag="VF226_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7074570.1"
                     /translation="MLSAFNVGDILSICLFLSVIGTLLIGYPIALTLAGTSLIFAVLG
                     HQFGAFDYAILNGLPLRYLGTMTNDVLVAVPLFIFMGLILEKSGLAEALLTTMGQLFG
                     PLRGGLAFSVVVVGALLAASTGVVGATVITMGLISLPAMLRAGYCPMLATGAIGASST
                     LAQIIPPSTVLIFAGDLLAGVNQAAQLKLGNFAPTPISVGDLFAGALIPGFILVGLFL
                     LWVVYKAVFQPETVPALVMSPEERRGLPVRVVIVLIPPLILIVAVLGSILFGFATPTE
                     SASVGAVGAMLLAAVNRKLSFATIRYASLNTMITTSIIFVIVLAASLFSLVFRGLGGE
                     HMVEQALHNLPGGAFGAMLAVMAIMFILGFFLDTFEIILIMLPICGPPLILLGLDPIW
                     IGVMIAMNLQTSFLTPPFGFTLFYMRGIAPPSVSTSEIWAGAIPFVFLQILGLSLLWA
                     FPQAATWLPEKLFGQPQIEQTAPAGNSDRGIGEFIQDGLKFDEEGIPLDDNPFSTNR"
     gene            complement(10043..10582)
                     /locus_tag="VF226_11065"
     CDS             complement(10043..10582)
                     /locus_tag="VF226_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7074571.1"
                     /translation="MRALAGFVRFAEAVNDVVGRTVAYLTLGTVLICATVVVIRYVFE
                     TGFIWLQELFVWTHSTAFMLGAAFTLMVNRHVRVDILYASRSQRTQALLDLLSTIVFL
                     FPWIIVLWYYAWPYVAASWALGEESPQVGGMPGLYLLKSVILGFCALMMLQGLAICAR
                     SAIVLGGRPELLPAQPTAD"
     gene            complement(10783..12504)
                     /locus_tag="VF226_11070"
     CDS             complement(10783..12504)
                     /locus_tag="VF226_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7074572.1"
                     /translation="MLKPAAIGTAILLLAAGSGQPASAIGELAAVKAQEAASALLRGN
                     LDQAVASYTDALQDQTLPNDRRAAILNDRGVASSRLGKHKAAIDDFNRAVQLFPEYAP
                     VYNNRGNTLLSLGLVEEAIKDFNRAIALAPGYAAAYNNRASAKLKLGEAEGAIEDYTQ
                     AVRLAPQNSAALTGRGQAYMQQDRPYSAMREFSRAITADSRSVPAYRARAHAEMQVGQ
                     YEQAIEDLSRAIAFEPENAELQIERGYAYRLADNAASALRDYSRALEIDPSNARALAG
                     RGLMLTKLASFEEAEAELSRALEIDPRSAEAFAYRAWFYKQTGQPELGLPDVEKALKI
                     DAGDADVHWVKGEVEQALGHTDIAIASLQKALSINPFHRDARMALQQMGVEPDAAEEV
                     PAAAIDGWRVVSENGRLVAHLQGRSNLTVPLERAGAGEPRLLEWELKDGVLKGIGLLR
                     YFAGAVEGPNGPEPIEQVAIVDVPASRVVSLQLHRRGDKESTWTWNEGTVTIESIDGV
                     TDVLVLRDTTPREVADRRPARTRQDFWDGPSWAPWAQDRVYRERRAREPRRSRPPKTI
                     FDFLFGN"
     gene            complement(12633..13709)
                     /locus_tag="VF226_11075"
     CDS             complement(12633..13709)
                     /locus_tag="VF226_11075"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEX7074573.1"
                     /translation="MANLSNNKMKIAVIPGDGIGNEVVPEGLRVLEAAGARFGLDYQW
                     DHFDWNCERFHKTGSMMPADGLDQLRPYDAIFLGAVGYPGVPDHVSLWGLLIPIRRTF
                     QQYINLRPVRMFEGLKSPLADRKVGDIDYLIVRENNEGEYSSIGGRLYEGTELEAAFQ
                     QAVFTKKGCERVMRYAFDLAMTRKREVTSATKSNGISISMPYWDEKFAAVAKEYPDVK
                     TQQFHIDILTAHLVRNPHWFDVIVGSNLFGDILSDLGPATTGTIAIAPGANINPEREF
                     PSMFEPVHGSAPDIAGKNIANPIGQIWSGAMMLDHLGHKEAADAIVKAIEEVIRAREA
                     LTPDMGGKATTQELGKAIADIVRG"
     gene            complement(13881..>14823)
                     /locus_tag="VF226_11080"
     CDS             complement(13881..>14823)
                     /locus_tag="VF226_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX7074574.1"
                     /translation="IVRDPQVRTRIQRLVIPPAWEDVRIAADPMAHIQAIGRDEAGRI
                     QYIYHPLWDRLREERKAKRLVEFAKALPAIRRSVKRDLSRRVGSYELAIAACVALIDE
                     TAIRVGEQVHLKRTGARGATTLRQQHVRIEGERILLCFPAKGGKELHAEVAAKRLADA
                     LQRLKRLRSRRLFCYKGHSGTLTCITARDINTYLSSISGRRVSAKDFRTFHATALAGD
                     RLAALTPEHSDRKRNSQIVSVVKEVAEMLGNTATIARKSYVHDVVLTSFANGQLVRRW
                     RRGGPLRRGLGAHESALARFLERVPLRSRGRRAGTTR"
BASE COUNT         2657 a         4596 c         4852 g         2718 t
ORIGIN      
        1 gtgctgctcg gcggtgtgcc gggcgtcgat cccgcgaagg tggtcgtgct cggcggcggg
       61 gtggtcggca ccaacgccgt ccacatagcc ctcggcatgg gtgccgatgt ctgggtgata
      121 gaccgcaacg tcgatgtcct gcgccggctg tggagccatt tcgggcggcc gctcaatacg
      181 gtctactcga cgcgcgatgc catcgaccgc catttgcggg atgcggatct ggtgatcggc
      241 gccgtgctga tccctggtgc cgaaacaccc aagatcatca cccgggagca cgtgaagatg
      301 atgcgtccgg ggtcggtcat cgtcgacgtc gccatcgacc aaggcggctg cgcggaaaca
      361 tcccggccca cgacgcacga caatccgacc tacaccgtcg acggcgtcat acactattgc
      421 gtggccaata tgcccggtgg cgtgccgaag acgtcgaccc aggcgttgaa caacgcgacg
      481 ctgccgttcg tcatcgcgtt ggccgagaag ggatggaaac gcgcactcgc cgaagacgag
      541 cacctgcgcc atggcctcaa catctgggac ggcaaggtaa cctgtaaacc cgtcgccgac
      601 gcccagggct acccttacct ccccgccgag gaggctctag gctgaatcgg tttctgccga
      661 tacgccgccc atcgcgcgat gccggtcgag cgctgttcgc gacgaaaagc atcaacacat
      721 ctccaatggc attcgacttg gttttgatgc taggcatggc gtatccgtgt cgatctgcgg
      781 gggagacatg ccagacgagc cagccgagaa gccgccggtg ctagaggtgc gcgacctcgt
      841 caagaatttc ggggggttgc gcgcgattga ccactgctcg ctcagcgtgc gcgaggggac
      901 gataaccggt ttgatcgggc caaacggcgc agggaagacg acgctcttca atcttgttac
      961 cgggtttttc cagcccgatt ccggccgcgt gctcgcagat gggaaggata tcaccagtct
     1021 gccgccgcac ggcgtcttcc ggcgcgggat ctgccgcacg ttccagattc cccgcgagca
     1081 cggcacgatg accgtccttg aaaatctcat gctggtcgct cccgaccagg ccggggagcg
     1141 attctggaac tgctggttcc gctccggcct cgtgcgccag caggagcgcg agattcgtga
     1201 ccgggcaatg gaggtgctcg agttcgtaga cctcgcgcgg ctcgcgaacg aatatgccgg
     1261 ccggctctcg ggcgggcaaa agaagctcct cgagctggcg cgcacgctga tggccaatcc
     1321 gcgcctcgtg ctcctcgacg agccggcggc cggcgtcaac cgcaccctga tgaagaagct
     1381 cgtcgacaac atcgagtatc tcaggcgtga gcgcggcatc acgtttctgc tgatcgagca
     1441 cgacatggac ctggtaatgg agctttgtga tccggtgatc gtgatgagcg agggccgccg
     1501 tttgatggaa ggccgccccg aggaggttcg ccgcgatccg cgggtgctgg aagcctatct
     1561 gggggggcag tatggcgctg cttgaagtcc ggggcgtcac cgccggctac ggcgacacgg
     1621 aaatcctgcg caatgtgtcg atggaagtgg gagagggcga gatcgtctcg atcatcggac
     1681 cgaatggtgc gggcaaatcg actttgatga agacgatatt cgggctgctg catccgcggg
     1741 aggggagcat catcttcgac gggcaggaca tctcccgtct gcagccctat cagatcgtcg
     1801 agcgcgggat gtgctacgtg ccgcaggtcg ccaatgtctt caccgacctc accgtgtcgg
     1861 agaatctcga gatgggcgcc ttcctcgcga agaccaacga catcgcggcg cgcaaggaac
     1921 ggatctacga gtatttcccg cggctcaagg agcgccgcaa tcagcgcgcc ggcaagatgt
     1981 cgggtggcga gcggcagatg gtggcgatgg gaagcgcgtt gatgctcgag ccgaagctcg
     2041 tattgatgga cgagccctct gccgggctat cgccgaagat ggtgggggtg atcttcgaac
     2101 agatccgtaa gatcaacgag gcgggcccgg cgatcctgat cgtcgagcag aacgccaagt
     2161 tgtcgctcga gatgtcccac aggggctatg tcctggccag tggcgagaat cgcttcgaag
     2221 gcacgggcga ggaacttctg aacgatcccg aagtcggccg gctctatctc ggcggctgag
     2281 gaaggaccga actgggggct gcaggatggc agaaattttg caggaagcgc cgggtcggag
     2341 cgacgaccgc acgacgcacg agcgcatcat ggcggcgctg cggagccggc cggcgctcgg
     2401 agtggcgctg tttttgacat tggtggtgtt ctggctgctc gggcagcagc aaggggcgtc
     2461 actttccgat ttgctgtcgc tgacggtttg gggggtcatg ctcggcggca tcatcgcgct
     2521 cggtgcgatc ggcctgacgc tggtctatgg cgtgctcaaa ttccccaact tcgctcatgg
     2581 cgagctggtg acccttggcg catacggcgc cctgacgatt tccgcgctgc tgccggcggg
     2641 gccggccatg caaccgttct ccttcggctg ggagctaatg gcggggctga tcctgtcgat
     2701 gccaatcgtc tccttgatcg cgctggtggt tgatcgggtc cttttccggc ccctgcgctt
     2761 gcgtttcgcc tccctcgtgc ttttcgcgat ggcctcgctc gcagccgcgt tcttcctgcg
     2821 cagtgcgatc tacctcgtgt gggggtcgga cttcctgttc tactatccgg gccgcgccaa
     2881 tccggcgatc gaaatgccgc tcggtatccg agtgcaggcc gatcagctct tcgttttcgg
     2941 gctggccgtc gtgctggtca tcatcacctg gttcctgctc gagcgcacgc ggatgggcaa
     3001 ggcgatgcgc gcaaccgcca ataatcccga tctggcgcag gtccgcggca tcaataccga
     3061 gagggtgatc gcctggacct ggattctcgg cgccagcctc gccgccgccg ggggtgtgat
     3121 gtacggcctg gcttctcagt tgcgaccaga gatgggcttt tggctgctcc tgcccatgtt
     3181 cgcggcggcg atcgtcggag ggctgggcag tcccataggg gctatcgcgg gtgcggtcat
     3241 catcggggtc gcccagcagg tgtcgtcctc gttcctcaac cctgcctacg ggccgggggt
     3301 cgcgttcatc ctgctggtga tcgtgctcgt gctgcagccg cagggccttt tcgggcaggg
     3361 gggagactga gatggcaaag gccgatctgg cgcgcgccgc ctttctcttc ctgccgctgg
     3421 tgttgctcgg gcttctggtc ctgccgggac gcatgaggct gcgtctgccg ctttcactgg
     3481 tgatagcggc ggtggtggcg ctggcgctgc ctatgctctt gccgcgcggc gccttgacct
     3541 acctcgcctc gttctcgacg atggcgggga tctacgcgat cctctgcctc ggcctgaaca
     3601 gccaatgggg actgacaggg cacgtcaatt tcggcattgc cggcttcttt gcgatcggcg
     3661 ccttcacctc ggccctcgtc accacgaaca tgccgcaggg tgcgctcgca ctctattccc
     3721 agcaggcgtt cgggctcggc tggccgatca tcgccggcat cctgacggcg ggcgtcgtgt
     3781 cggcggtggc gggctttctc atcagcattc ccgtgctgcg cctgcgcacg gacttcctcg
     3841 ccatcgcgac cctcggcatt gccgaggtca ttcgtttcat cttccagaac gagcgctggc
     3901 tcgctaacgg accgcagccg atgcgcggca tacctcggcc gttcgactgc gttttcaccg
     3961 atccgccgtg tgcctggctg ccggagggtg tcgcgcgctt cgtcgcgccg cttgcaccgc
     4021 gagactacgt atacctctac ctcgtgatcg tggcgatcat cctcgcggtg atattcgtgc
     4081 tgctcgaacg catctcgcgt tccccgtggg ggcgcctcca gcgggcgatc cgcgacgagg
     4141 agaactcggc ggcgatgagc ggcaagaacg tgcccttacg ccgggtggag tccttcgttc
     4201 tgggcagttt catcatgggc gtcgccggcg cggtctatgc ccattacgtg gtcagcatcg
     4261 attacagcca cttcaacccg ctgttcgcga ccttcatcgt ctgggtgatg gtgatgctgg
     4321 gagggagcgg caacaacaag ggcgccgttc tcggagcatt cgcggtctgg ggtgtgtgga
     4381 gcggggcggc cttcatgatc gatgtgatga gcccggcgct ccaggccatc tctcccgacc
     4441 tgccgtcgcg cggggcctat ctgcggtgga tgttggtcgc ggtgatgctc gggctgatcg
     4501 tgctgttccg tccgcagggg atcctcgagg agcgcaagaa ggtctcggac ttcatgtgac
     4561 ggccgattag atcatttccg acatagatgg aagcaagcta acctaagctt gcgccgatct
     4621 ccctcccccc ggtggggaga gggtcggggt gagtgggttc cgccagtggc gatcgttgca
     4681 acccctcacc cggcccttcg ggccgacctc tccccatggg agaggtaagg gagactgctg
     4741 cctgcctaat ttggaaatgc tatagcggcc ttggcaaaaa aaagggcgcc tgaaggcgcc
     4801 cgtaattgct aagtaaagcg tccgctattc cgaggcaatc tgatcggcag ggacgatctt
     4861 caccgtctcg tcgcctgtct cggtgaactt ccagaccgcg attggcgtct tgacgtcgcc
     4921 attctggtcg aagtcgaccg aaccgcctgc gccgacgtag tcgatctcct tcccttcctt
     4981 gagcaactgc aacgccttcg tgatctgttc ctggctgccg ccgatgatct tctcgcctgg
     5041 tgggttggcg atcgggcgca ggcgatcacg aagcgcggtg ccggtgatct cggcgggatc
     5101 cttgatccca tcggcgaccg ccttggccag cgccagcccg attaccatcg ccgcgtcata
     5161 ggtcgtctcc gagaagggcg ggatgcggtc ctgcttggtg gcggcgcgat aggcctccgc
     5221 gaaggtcttg tagctttggg attcagggtc ggccgaaggc accgtgccga gcttgcctgc
     5281 cagatccttg ccgcccaccg catcgagcac ctcgctggac cggttggcat cggtgaactg
     5341 ccagctcgtg tagccgaaga tgtcgcggct ttccttgagg atctgggcgg tatgggccgg
     5401 atacgtcggc agcaccagca cgccgggctt gccctgcagc gccgtgctca gaatggagcg
     5461 gtacgtgggc tgcacctcct cggcgtgggc aacctgggca agcacctttc cgccgcgctt
     5521 ctcgaaggca gcggcgaagg cgttggacag gccctggccg tagggattgt tgatgtagat
     5581 ggtcgccgcg gtctcgaact tgtagccgtc gatgagcttg ccggcggcga ggtccgccgc
     5641 gacgacgccc tgcagcgcgt ccgaggggct ggtacggaac agcagatcct taccctcgtc
     5701 ctcggggagg accgaaacca gcggcgaggt cgtgccgttg ccgacctgca gcacgcccga
     5761 gggaatagtc accgacgtgg ccaccgccac cgtcacgccg ctcgaccact cgcagatgat
     5821 ggccgggacg ccgtcggcct cgaccagctt gcgcgccgcc tcgatggcag gcgtcggcag
     5881 agtgttggaa tcttccgcga tcagccgctc gacgatcggc ccgccgaaaa cctcctcggc
     5941 ggcggtgttg atctggttca gggccagttg cgccgcgtca cggaagaacg ggccgtattc
     6001 ggcgcctgag ccggtgaacg caacgacctg tccgatgggg accttcggcg gcgcgctctg
     6061 ggcccagagt gcgcggggca tcgtgacgct cagcgtcatc gccccgccgg cggcgccgac
     6121 agctttgaga aaatcgcggc gacgggcatt tctgagtgaa aaatcggaca tctaatcctc
     6181 ccaatgagat gcgcgggaat cggccctgac gaaatcgagt cccggccaag gcgagctctc
     6241 aagccatacc acaatcgtga acgagagcca ccacttcatc aagacccggg ctggcatcta
     6301 gcatcttgcg gtcagcggcg gctgacggtc gggtatgcgg ggagggaccg cgggtaagcg
     6361 agcgagcggc cggcgatggc ggcagggcag atgatcttgc gcccgcgaac atcactccac
     6421 cggcgcgata ggacgccggt ggttcaggtg actaaggcat ttacttcgag gaagacgggt
     6481 tcttatcgcc cttgcgattg cgccatgaac tccgtcatca ggtggcgctc gcaacgccgc
     6541 agagcttcag gcaccggccg cccggcagcc gaataggacc gaatgcgatc ctgtacgcgg
     6601 gcatacgcat cacgcagatc ttcaaagtca tcgagctccg cgaacagctc tacgcattcg
     6661 cttggaagct tttcgcctcg attacccatc gacttctccc acacaagctg cgttttcctc
     6721 ctcccccatt tcaggaattg ttgccatgca acggagcaaa aaccgttgac atgaaacaac
     6781 ttcggatcac ggctctgatt ttatttgcag cgcaacattt ctccagattt gccgccgttc
     6841 tccgcagata gttaaacgaa gatagagatc aacgtccagt ggtaatagcc gctgaacgcc
     6901 gtgtgcggcg aaacgcagcg ccaagcgagc gattttggtg aggcggaacc cgggaccact
     6961 gacgtcagcc atcgaagtga gttgcctaat gctcaccaca aaatcatact cagctcacgg
     7021 ctgagacagc cgcaatatca actcgagtgg ggcagcccag ggagcaagac gaatgacaat
     7081 cagacgccgc cagtttctcg ccggcgtggc gacggccggt gcagccgccg gcgcgacggc
     7141 gctcgcgaaa cctgccatcg cacaaggcaa aaccgaatgg cgcatggtga cgacgtggcc
     7201 gaagaacttt cccggccagg ggaccggtgc cgagcgcttc gccaagacgg tcaccgaggc
     7261 gaccgatggc cgcctaacga tcaaggtata cgcggccggc gagatcgtac ccgcgttcga
     7321 gagcatcgat gcggtcatcc gtggtgctgc ggacctgatg cacgcgacgc cgtactattg
     7381 gccgaataag agcaaggcac tgaacttttt ctcaaccgtg cccttcggca tgacgacgcc
     7441 agagctcgac gcctggatga accatctggg cggccagcaa ctgtgggaca agctctacga
     7501 ccagttcggt ctcaagccct tccacgccgg ctcgacgggc gtgcagatgg gcggttggtt
     7561 caacaaggag atcaccggcg tcgattccgt caaaggcatg aagatgcgca ttccgggcat
     7621 cggcggcgag gcgctgggca agctcggcat gaccatcgtc aatctgccgg ccggcgagat
     7681 cttcgctgcg ctccagtccg gcgccatcga cgcgaccgaa tgggtcggcc cttacaacga
     7741 cctggcgctc ggcttctaca aagtcgccaa atactattac tggcccggca tgcacgagcc
     7801 cggcacgcag atcgaagtca cggtcgacaa ggccaagttc accgctctgc ccaaggacgt
     7861 gcaacgcatc ttcgaggcct gctgccacaa cgagaacaat ctgttgacgt cggagttcaa
     7921 cgcgcgcaac ggcggcgcgc tggagacgct catccggcag cacaacgtgc agctcaagcg
     7981 cttcccgaac gatttcctgc aggcttacgg caatgccgcc ggcgaggtca tccaggaaat
     8041 gcgggacagc ggcgatccgc tcacgaagga aatcgtcgag tcgttcttga aggctcggcg
     8101 cgacctcatg aactgggggc ggattgccga ccagggcttc atgaacgccc gcctgctgga
     8161 catcaagttc ccgacctgat ccagagggca acgatgtatc tgcggggccc ggcgacgggc
     8221 cccgttctta tttgacggtg ctcatcaccc cacgacatgc agacctgtga ggaccattgc
     8281 tatcccgacc acgagcacgg tcatgtgcac gagcatgagg cattctttcg caagtctgct
     8341 tttgatcgga cctggacggt tctgcctgtc gatcaacctc cacaatgcga acagggtgag
     8401 gaaccacagc accagcaggc cggctccgat ctcgatgctt gtcaggtctt ccatggcctc
     8461 cgcccaggtc agcgattggt ggaaaacgga ttatcgtcca gcggtattcc ttcctcgtcg
     8521 aacttgaggc catcctggat gaactcgccg atgccgcggt cgctgttgcc agcgggggcg
     8581 gtctgctcga tctgcggttg gccgaacagc ttttccggca gccaggtcgc tgcttgcggg
     8641 aacgcccaaa gcaggctcag tccgaggatt tgcaggaaga cgaacgggat ggcgccggcc
     8701 cagatttccg aggtcgagac gctgggtggc gcgatgcccc tcatgtagaa cagcgtgaag
     8761 ccgaacggag gggtgaggaa gctcgtctgc aggttcatgg cgatcatcac gccgatccag
     8821 atcgggtcca gtccgagcag gataaggggc ggcccgcaaa tcggcagcat gatgagaatg
     8881 atctcgaacg tgtcgagaaa gaagccgagg atgaacatga tcgccatgac ggcgagcatt
     8941 gcgccgaacg cgccgccggg cagattgtga agtgcctgct cgaccatgtg ctcgccgccc
     9001 agcccgcgga aaacgagcga gaagagcgag gccgccagaa cgatgacgaa gatgatcgaa
     9061 gtggtgatca tcgtattgag ggaggcatag cggatggtgg caaaggacag ctttcgattg
     9121 acggccgcca gaagcatcgc gccgacggcg ccgaccgacg cggactccgt cggggtcgcg
     9181 aatccgaaaa gaatgctgcc tagaacggcg acgatcagga tcagcggtgg aatgagcacg
     9241 atgaccaccc gaaccggcag acccctgcgc tcctcgggcg acatgacgag tgccggaacc
     9301 gtctcgggct ggaagacggc cttgtagacc acccagagca ggaacaaccc gacgagaatg
     9361 aaaccgggaa tgagcgcccc ggcgaacagg tcacccacgg aaatcggggt cggggcgaag
     9421 ttcccgagct tcagctgcgc cgcttgattg acgccggcca ggagatcgcc ggcgaaaatg
     9481 agcaccgtcg acggaggtat gatctgtgcg agcgtcgagg aggcgccgat cgcacctgtt
     9541 gccaacatcg ggcagtagcc tgcgcgcagc atggcgggca ggctgatcag gcccatggtg
     9601 atgacggtcg cgccgaccac acctgtcgaa gccgcgagca gggcgcccac gacgacgaca
     9661 gaaaaggcga gccctccgcg taacggcccg aagagctgcc ccatggtcgt gagcagcgcc
     9721 tcggcaagcc ccgacttctc caggatcagc cccatgaaga tgaacagcgg cactgcgacc
     9781 aggacgtcgt tggtcatggt gccgagatat cggagcggca gaccgttgag gatcgcgtag
     9841 tcaaaggcac cgaactgatg gccgagcact gcgaagatca gtgacgtgcc ggccagcgtg
     9901 agcgcgatcg gataccctat gagcagcgtg ccgatgaccg acaggaaaag gcagatggat
     9961 aggatgtcgc cgacgttgaa ggctgaaagc atgggctgat tcgctcaagg ctgctgttga
    10021 tgggccagac gctggcggcg ggtcagtcgg cagtgggctg ggcgggcagc agctcgggcc
    10081 ggccaccgag gacgatggcg ctgcgggcac agatcgccag tccctgtagc atcatgagcg
    10141 cgcagaagcc gaggatgacg gacttgagca gatagaggcc gggcatgccg ccgacttgcg
    10201 gcgattcttc tccaagcgcc catgacgcgg cgacataggg ccaagcatag taccagagca
    10261 cgatgatcca ggggaacagg aacacgatcg tggacagaag gtcgagcagg gcctgcgtcc
    10321 gctgggagcg ggacgcataa aggatgtcga cacgaacgtg ccggttgacc atcagcgtga
    10381 aagcggcgcc gagcatgaac gccgtggagt gcgtccagac gaacagctcc tgcagccaga
    10441 tgaagcctgt ttcgaagaca tagcggatga cgacgacggt ggcacagata agcaccgttc
    10501 ccagtgtgag ataggcaacg gtgcggccca ccacgtcgtt gacggcttct gcgaagcgca
    10561 cgaagcctgc cagtgcgcgc atatgcgttt ctctccccta ggcgtcgtcc cctctttttg
    10621 cgcgaactct aacagccaac cgaccgcgcc gccaagcgtt ctgcgtcgtg gtgccaatcg
    10681 ccggtgcaaa tgcggcctcc cggcgccgga gatcatcctt gtgacgcgcg cgccaagcgc
    10741 ctatgccgac ggggagggaa tcagagtgct gatgcgaacg gctcagtttc caaacaggaa
    10801 atcgaagatg gtcttgggcg gtctgctccg ccggggctcc cgggcacgcc gttcccggta
    10861 gactcggtcc tgggcccatg gcgcccagga cgggccgtcc cagaagtcct gtcgagtgcg
    10921 cgccgggcgg cgatccgcga cttcgcgcgg agtcgtgtcg cgcagaacga gaacgtccgt
    10981 gacgccgtct atgctttcga tcgtcacagt accttcgttc cacgtccaag tggattcttt
    11041 gtctccgcgc cgatgaagct gcagactgac gacccggctt gcgggtacgt ccacgatcgc
    11101 gacctgctcg atcggctccg gcccgttcgg tccctcgacg gcgcccgcga aatagcgcag
    11161 caggccgata cccttgagga cgccgtcttt cagctcccat tcaagcaggc gcggctcgcc
    11221 cgcacctgcc ctttcgagag gtacggtcag gttcgaccga ccttggagat gagccacgag
    11281 ccggccattc tcggagacga cgcgccagcc gtcgatggcc gccgctggga cctcctcggc
    11341 tgcgtcgggc tcgaccccca tttgctgcag ggccatacgg gcatcgcgat ggaaggggtt
    11401 gatcgagagc gccttctgca agctggctat ggcgatatct gtgtgcccga gcgcctgctc
    11461 gacctctcct ttaacccagt gcacgtccgc gtcgccggcg tcgatcttca gggctttctc
    11521 gacgtccggg aggccgagct cgggctggcc ggtctgcttg tagaaccagg cgcgataggc
    11581 gaatgcctcc gccgaccgcg gatcgatctc cagcgcccgg gaaagctcgg cttcggcttc
    11641 ctcgaaggac gcaagcttgg tcagcatgag tccgcggcct gccagggcgc gcgcattgct
    11701 cggatcgatc tccagtgcgc gtgagtagtc ccgcagcgca gaggcggcat tgtcggcgag
    11761 ccggtatgcg tagccgcgct cgatctgcaa ctcggcattt tccggctcga atgcgatcgc
    11821 gcggctgagg tcctcgatgg cctgctcgta ctgccccacc tgcatctcgg cgtgggcgcg
    11881 ggcgcgatag gctgggaccg agcggctgtc ggcagtgatc gctcggctga actcgcgcat
    11941 cgctgaatag ggcctgtcct gctgcatgta tgcctgcccg cggccggtca gagcggcgga
    12001 gttctgggga gcgagccgca cggcctgcgt atagtcctcg atcgctcctt ccgcctcgcc
    12061 gagcttcagc ttggcgctgg cgcggttgtt gtaggccgcc gcatagccag gagcgagggc
    12121 gatggcgcgg ttgaaatcct tgatcgcctc ctcgaccaat ccgaggctca gcagcgtgtt
    12181 gccgcggttg ttgtagaccg gcgcatattc cggaaagagc tgaacagcgc ggttgaaatc
    12241 gtcgatcgcc gccttgtgct tgccgagccg gctgctggcg acgccccggt cattgaggat
    12301 cgcggcgcgc cggtcattcg gcagcgtctg atcctggaga gcgtcggtgt aggatgcgac
    12361 ggcctgatcc agattcccgc ggagcagggc cgacgcagcc tcctgggcct tcactgccgc
    12421 cagctcgccg atggccgacg cgggctggcc cgagcccgcg gcgagaagca ggatggcagt
    12481 cccgatggcg gctggtttca gcatgtcggc cccagtattc cctcgcccgc cccggtggaa
    12541 atcgccccac tataacgcag gacggtgcaa aaccggatag ggcaggcggg tcgagggtta
    12601 tctcacccgt cccgccaagc cgcgagcggg catcagccgc ggacgatgtc ggcgatcgcc
    12661 ttgccgagct cctgcgtcgt ggccttgccg cccatgtcgg gcgtcagcgc ctcgcgcgcc
    12721 cggatcacct cctcgatggc cttgacgatg gcgtcagcgg cttccttgtg cccgagatgg
    12781 tcgagcatca tggcacccga ccagatctgg ccgatcggat tcgcgatgtt cttgccggcg
    12841 atgtccgggg cagatccatg gaccggctcg aacatggaag gaaactcgcg ctcgggattg
    12901 atgttcgcgc ccggggcaat ggcaatggtg ccggtggtcg ccggtccgag gtccgacagg
    12961 atgtccccga acaggttcga gccgacgatg acgtcgaacc aatgcgggtt gcgcacgagg
    13021 tgtgcggtga gaatgtcgat gtggaactgc tgcgtcttga cgtcggggta ttccttcgcg
    13081 acggcagcga acttctcgtc ccaatagggc atcgagatcg aaatgccgtt cgacttggtc
    13141 gccgatgtca cctcgcgctt gcgggtcatg gcgaggtcga aggcatagcg catcacgcgc
    13201 tcgcaacctt tcttggtgaa cacggcctgc tggaatgccg cctcgagctc cgtgccctcg
    13261 tagagacgcc cgccgatgga ggagtattcg ccctcgttgt tctcccgcac gatcaagtag
    13321 tcgatgtcac ccaccttgcg gtcggcaagt ggcgacttca gcccctcgaa catgcgtacc
    13381 ggccgcagat tgatgtattg ctggaacgtg cgacggatgg ggatgagcag cccccagagc
    13441 gagacgtggt ccggcactcc gggatagccg accgcaccga ggaaaatggc gtcgtaaggt
    13501 cggagctgat cgagcccgtc cgcgggcatc atgctgccag tcttgtggaa gcgctcgcag
    13561 ttccagtcga aatggtccca ctggtagtcg aggccgaagc gggcgccggc ggcctcaagc
    13621 accctcagcc cctcaggcac cacctcgttg ccgatcccgt ccccggggat gacggcgatc
    13681 ttcattttgt tattggacag attggccatt tggagctgct ccactgctgt ttaggacggc
    13741 ggactgacct cgccgctgtt gacgcgtaaa ggcgatccta tcggagcgcc cattcgcaga
    13801 tcgtttagcg gagttggagc gcgacgccaa tcgcaggctg cgggtgcgag gccgcaagag
    13861 ttgaaggctt aggagccttg ctacctggtt gtcccggcgc gccgtccccg ggatcgcaac
    13921 ggcacccgct ccaggaagcg tgccagcgcc gactcgtgcg cgccgagccc tcgccgtagc
    13981 ggaccgccgc gccgccagcg gcgcaccagc tggccgttgg cgaaactcgt cagaacgacg
    14041 tcgtggacgt agctcttgcg cgcgatggtg gccgtgtttc cgagcatctc cgccacctct
    14101 ttcacgacgg agacgatctg gctgttgcgc ttgcggtctg agtgctccgg ggtgagcgca
    14161 gcgaggcggt ctcctgcaag cgccgttgca tggaaagtcc ggaagtcctt cgcgctcacg
    14221 cgccggccgg aaatggacga gagataagtg ttgatgtccc gcgcggtaat gcaggtcagt
    14281 gtgcccgaat gccccttgta gcagaagagc cgccgcgagc gcaggcgctt caaacgctgc
    14341 aacgcatcgg cgagacgctt tgcggcaact tcggcgtgga gctccttgcc acctttggca
    14401 ggaaagcaga gcaggatgcg ctcgccctcg atgcggacat gctgctggcg cagtgtcgta
    14461 gcgccgcgcg caccggttcg cttcagatgc acctgctcgc cgacgcggat cgcggtctcg
    14521 tcaattagtg ccacgcacgc ggcaatggcc agctcatagg acccaactcg tcgtgagaga
    14581 tcgcgcttga cgctccggcg gatcgctggc agcgccttgg cgaactcgac gagccgcttg
    14641 gccttgcgct cctcgcgcag tcggtcccag agcgggtgat aaatgtactg aatgcgcccg
    14701 gcctcgtccc gcccgatggc ctggatatgc gccatgggat cggccgcgat acgcacgtcc
    14761 tcccaagccg gcgggatcac cagccgctga atgcgcgtgc gcacctgcgg atcccgaacg
    14821 atg
//
LOCUS       DASVDU010000176         9436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1249370, whole genome shotgun sequence.
ACCESSION   DASVDU010000176 DASVDU010000000
VERSION     DASVDU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9436
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249370"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF226_11085"
     CDS             complement(<1..515)
                     /locus_tag="VF226_11085"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7074575.1"
                     /translation="MVEKSAPNARIPLPDLKQLDAEQQRVADEVASGPRKEVRGPVHV
                     WLHSPELASRAQKLGEFLRWGTTLDARVSELVILVTARHYDCRYMWNNHVGLALKAGL
                     TQEVIDCIDAGRAPSFAKTDEAVAYAFTTEMLERNTVSDATLAQARNLLGDRGVVEMG
                     ALIGHYHNGAIA"
     gene            complement(529..1533)
                     /locus_tag="VF226_11090"
     CDS             complement(529..1533)
                     /locus_tag="VF226_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7074576.1"
                     /translation="MPVPETNFSPPFNITRASHIRLTVRDLPRTREFYTEVIGLLVTD
                     EDTNIAYLRGVEETCHHSLVLKSTNDAPVCEAIGFRVFRDEDLDAAKSYFEQKGIEAR
                     WTKPDFQGRTLELRDPAGIPIELCASMPTQPRLHDKPHLHRGAAALRYDHVQCLMPDV
                     AGAGRFYTDLGFRISDYFVDRPEDEEPLGLFLYRKNNPHDLVFLTRPGPTMHHFGYIV
                     SDANCLFRALDTAGGTGFARNFERGPGRHGQGHAFYVYFRDPDGHRVEILPPPIQLID
                     FDDGPVKWHAGNRFTWDLPPPRKWLHESTPFQGTPISTPAHDPKLFSLEDFLAAQELS
                     "
     gene            complement(1580..2368)
                     /locus_tag="VF226_11095"
     CDS             complement(1580..2368)
                     /locus_tag="VF226_11095"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074577.1"
                     /translation="MGELRVEHLSKTYARRDGWNRTRYLRVFEDISFTVRDGEFVSII
                     GSSGCGKSTLLSIAGGLSEPTGGAVYVDGNQVKGPGLDRGIVFQEFALFPWLTVMGNV
                     AFGLNSKGLPKEEKIRLARKYVDLVGLSGFEDYHPHRLSGGMRQRVGLARALAIEPSA
                     LLMDEPFGALDAQTRESMQNGLSDIWNTTRNTILFITHDIREAIYLSDRVLVMSGRPA
                     NISLELTIDLPRPRHRHQAVFQDYEARLEAAIGQRHDSAGSKAA"
     gene            complement(2369..3220)
                     /locus_tag="VF226_11100"
     CDS             complement(2369..3220)
                     /locus_tag="VF226_11100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074578.1"
                     /translation="MAALDSDRRLAPVLSAEEVRTGGGLIGGLKNLAFLAIPIIVLLV
                     LWELVARLSGMPAALFPPASAIFSALVRMAAQGTLWLDIWASFSRLIQSIVLGSLVGT
                     LIGVLMGYYRIWERILAPPLNFLLAIPGTALFPLSMIWFGLTEFAITSILIYEVALTV
                     ILNTWTGVRTVDGSLIRAGKAFGATGPSLFWRVLIPAALPSMIAGYRLAFSRCWRILI
                     IAEMLVSVTAGLGYRIYWGREFFNSDVVYAGLLVVGIIGLIMERVFLRGLEVATVERW
                     GTMREAN"
     gene            complement(3241..4233)
                     /locus_tag="VF226_11105"
     CDS             complement(3241..4233)
                     /locus_tag="VF226_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074579.1"
                     /translation="MKRLAILALALSGLLVLPHLSSAQTKVTVGMASGVNQVTSLVAA
                     EQGYFKEEGLDVEIKPVVRGVLAVEAIVAGSMQFAEVSDVVLFSAIDKGIPLVALGAA
                     SRGFTGKMVGVPSLGEVKSLADLKGKRIGIQVGTGVHGVFLILLEQQALSESDFQISN
                     VRVTDMPTAMASGGAFDAVLGWDPMMQRIVQAGNGKEIISAKQFQEMAEITYPLLLVA
                     DKNYIAENRDTVQHFVNAYAKAHKWIRANPDGAFRIFSKTVRETGSALEDDIIKTMMF
                     EVDKFTGVNFIASDEKELSRTRDFLRKTGKISSAPDLKDVLEPSFADAAEAAIK"
     gene            4431..5309
                     /locus_tag="VF226_11110"
     CDS             4431..5309
                     /locus_tag="VF226_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7074580.1"
                     /translation="MRYVAFEEGGRARLGAVVGHEIIDLSPLTGAAVSVRAIVESADL
                     QSKAAKHASNTTASRLPLKSCRLLSPLPDPGKFICLGLNYADHAKEGGHSVPDYPSLF
                     LRATTSLIGPQDPIVAPRCSERLDYEAELVIVIGKRCRHVTEQAALDVIFGYTAFNDG
                     SVRDYQRRTTQWTAGKNFDGTGALGPWIVTADEMPPGAAGLNIRSRLNGNVMQSSNTD
                     RMIFSVPRTIAILSEIMTLEPGDLIATGTPEGVGHARTPPVWMRPGDEISVEIDGIGV
                     LTNRVVAEAEDSTIAA"
     gene            5318..6157
                     /locus_tag="VF226_11115"
     CDS             5318..6157
                     /locus_tag="VF226_11115"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7074581.1"
                     /translation="MIRAELNGEPGRSQTSLIYDRLRDDIVQGRFAPGSKLKIEELKA
                     RYDGGATPIREALSLLAADGLVERLEQRGFRVAEASAREFEELLAIRCWAEERALRLS
                     IKHGGNDWEEGIVLARYRLAHSKRVPLEVSHSTHADWERNHKKFHMSLVSACGSTTLL
                     RLCSQLYDENCRYRYIARLSDHERPDVYGEHEEIAEAVLARNADLAVKLIIAHYTRTG
                     DLLRKSLPAYLKGSVIKSASESRDSYGPQANILSADGTRKRSRPKLIRVTPKPRNRRA
                     ARE"
     gene            6161..7198
                     /locus_tag="VF226_11120"
     CDS             6161..7198
                     /locus_tag="VF226_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEX7074582.1"
                     /translation="MQKLIPTTVVGSYPQPDWLVDREKLKSQSVPRVRVTDIWRIEER
                     WLEAAQDDATVLAIRDMERAGIDIITDGEIRRESYSNRFVTELEGVDSRNPAIVTLPT
                     GRQGALPRIVSKIRRTHAVEVRDVEFLRRNTDRMIKITLPGPFTMSRQCHNEFYKDPD
                     EMVMDFAAALNAEAKELEKAGADVIQLDEPWLRQDPEAAKRVAVPAINAALEGLSATT
                     AVHLCFGYAHLVSGKPGGYAFLAELADTIADQISIEAAQPKVDLGVLEDLSGKSIMLG
                     VIDLGDPEIETPEKIAERLRAGLKHLPSERLIAAPDCGMKYLSRDVAFGKLKAMVDGA
                     AIVRAELQGAA"
     gene            7258..7914
                     /locus_tag="VF226_11125"
     CDS             7258..7914
                     /locus_tag="VF226_11125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7074583.1"
                     /translation="MKRLTYTFDNGPWPGATDRLLDFLKSRNIRATFFVVGERLNDPA
                     ARRLAERARAEGHWIGNHTLSHGDPLGFSGGLERVRKEIGEAEKLLGPLSHPRKFFRP
                     NGKGALGPHLLSPEAVEYLTEHRYTVVTWNNVPRDWDETRAGWADRAEATLEAQDWSV
                     LVLHDRFIAPMMDSLERFHDRLLARSVAIVQDFPKACVPIRDGRLEGDISGLVTQDPP
                     "
     gene            7928..8494
                     /locus_tag="VF226_11130"
     CDS             7928..8494
                     /locus_tag="VF226_11130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX7074584.1"
                     /translation="MKVLGISGSLRERSFNTALLRATQELAPPDMQIEIFDLAPIPIY
                     NDDVRLAGYPPEVSKFRDAIRAADGVIIASPEYNRSIPGVLKNAIDWASRPPDQPFAC
                     KPMAFMGVSNGALGAAFANHHLRQIFVYMDARMVNGPEVMIGDARSKFDASLNLVHEP
                     TRKFVSDHLKKLAALIRMTKGTVRPMPL"
     gene            8933..9142
                     /locus_tag="VF226_11135"
     CDS             8933..9142
                     /locus_tag="VF226_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074585.1"
                     /translation="MTTTINTVYVGTLAPLGPDCVPSGIFKRPAKGPWHIMRTGIVGD
                     QQGDARHHGGPEKAVHHYPYEHHAA"
     gene            9161..>9436
                     /locus_tag="VF226_11140"
     CDS             9161..>9436
                     /locus_tag="VF226_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7074586.1"
                     /translation="MADFLSSPPAFGENLSTLGITEETVHIGDIYQVGGVILQVSQGR
                     QPCWKLNARFNRPDMAWRVQMTGRTGWYYRVLREGWIETGDEFVLVER"
BASE COUNT         1959 a         2805 c         2878 g         1794 t
ORIGIN      
        1 gcgatcgctc cattgtgata gtgcccgatg agggcgccca tctccaccac gccccggtcg
       61 ccaagcagat tgcgggcctg ggccagcgtc gcatcgctaa cggtgtttcg ctcgagcatt
      121 tcagtcgtga aagcgtaagc cacggcttcg tcggttttcg cgaaagacgg ggcccgaccg
      181 gcatcgatgc agtcgatgac ctcctgtgtt aggccggcct tcagtgccaa cccgacgtgg
      241 ttgttccaca tgtagcggca gtcgtagtgt cgcgctgtga cgaggatcac cagctcggaa
      301 acgcgggcat cgagcgtcgt cccccaccgc aggaactctc cgagcttctg agcgcgtgag
      361 gcaagctcag ggctgtgaag ccaaacgtgc accggcccgc gcacctcttt gcggggaccg
      421 cttgcaacct cgtcggccac gcgctgctgt tcggcatcga gctgcttcag gtcgggaagc
      481 gggatgcgcg cgttcggcgc ggatttctcc accatcaggt catcactttc aggacagctc
      541 ttgcgctgca agaaaatctt ctagcgagaa cagctttgga tcatgtgctg gcgtgctgat
      601 cggcgtgcct tggaaaggtg ttgactcatg cagccatttc ctggggggcg gaaggtccca
      661 ggtgaagcga ttgccagcgt gccatttgac cggcccatcg tcgaagtcga tgagctggat
      721 cggtggcggc agaatctcaa ccctgtgtcc gtccgggtca cggaaataaa cataaaaggc
      781 atggccctgc ccatgtcggc cgggcccgcg ttcgaaattc cgcgcgaaac cggtgccgcc
      841 tgcggtatcg agcgcacgga acaggcaatt ggcatccgag acgatatagc cgaaatggtg
      901 catcgtcggt ccagggcgcg tcagaaagac gaggtcgtgg ggattgttct tgcggtagag
      961 gaacagacct agtggctcct cgtcctccgg gcgatcgacg aagtagtcag agatgcggaa
     1021 gccgagatcc gtgtagaagc ggccagcgcc cgcgacgtcc ggcatcagac attgcacgtg
     1081 atcatagcga agcgccgccg cgccgcgatg aagatgcggt ttgtcgtgca gccgtggctg
     1141 ggtcggcatg ctggcgcaga gctcaatcgg gatgccggca ggatcacgca gctcgagcgt
     1201 tcttccctgg aagtccggct tggtccagcg ggcctcgatg cctttctgct cgaaatagga
     1261 cttggccgcg tccaggtcct cgtcccggaa tacacggaaa ccaatagcct cacagacggg
     1321 ggcgtcgttg gtcgatttca ggaccaagct gtggtgacac gtttcctcga caccgcgaag
     1381 ataggcgata ttggtgtcct catccgtgac cagcaggccg ataacctcgg tgtagaattc
     1441 gcgcgtgcgc ggcaggtcgc gcaccgtgag ccggatatgg ctggcccggg tgatattgaa
     1501 ggggggagag aaattggtct cgggtacggg catacttcaa tgcactccga aagtgcgccg
     1561 atggtgcgaa tttaggccgt taggcagctt tgctgccagc gctgtcatgg cgctgaccaa
     1621 tggccgcctc cagacgcgcc tcatagtcct ggaacaccgc ttgatggcgg tgacgcggac
     1681 gcggaaggtc gatcgtgagc tccagcgaga tgtttgcggg ccgcccgctc atcaccagaa
     1741 cgcgatcgga aagataaatg gcttcgcgaa tatcgtgggt aatgaagagg atcgtattgc
     1801 gggtggtgtt ccagatatca ctcagaccgt tttgcatcga ctcacgagtc tgtgcgtcga
     1861 gggcgccgaa gggctcgtcc atgagcagcg ctgatggctc gatcgcaagc gcccgggcaa
     1921 gcccgacccg ctggcgcatg ccacctgaaa ggcggtgagg atgatagtcc tcgaaacccg
     1981 aaagcccgac gaggtctacg tacttccggg cgagccggat cttctcctcc ttgggcaggc
     2041 ctttggaatt cagtccgaag gcgacgtttc ccataactgt cagccaaggg aaaagcgcaa
     2101 attcctggaa gacgatcccg cggtccagcc ccgggccttt cacttggttc ccgtccacat
     2161 agacagcacc gcccgtgggc tcggataggc ctcccgcaat gctcagcagg gtagacttcc
     2221 cgcagcccga cgagccgata atgctgacga attcaccgtc gcggaccgtg aaggaaatgt
     2281 cctcgaagac acgaagatag cgcgtgcggt tccagccgtc ccggcgggcg taggtcttgc
     2341 tgaggtgttc gacccgaagc tctcccattc aattggcctc cctcatcgtc ccccaacgct
     2401 ccactgtggc cacttccaat ccacgcagga acacgcgctc cataatgaga cctatgatgc
     2461 caacgaccag cagacctgca tagacgacgt cggagttgaa gaattccctt ccccaataga
     2521 tgcgatagcc aagtccggcc gttacggaga ccagcatttc ggcgatgatc aaaattcgcc
     2581 agcaccgcga aaacgcgagc cggtaaccgg cgatcatgga aggaagtgcc gccggtatca
     2641 ggacgcgcca gaagagcgac ggtccagtgg cgccaaaggc tttgcccgca cggatcaagc
     2701 tgccgtcgac cgtacgcaca cccgtccacg tattcaggat tacggtaagc gccacttcgt
     2761 agatgaggat gctcgtgatg gcgaactcgg tgaggccgaa ccagatcatc gacaagggga
     2821 atagggcggt tcccggaatg gcgagaagga agttcagcgg cggcgcaagt atccgttccc
     2881 aaatccggta ataccccatg aggacgccga tgagcgtgcc gacaagagag cccaaaacga
     2941 tgctttggat gagccggctg aaggaggccc atatgtcgag ccatagagtc ccctgcgctg
     3001 ccatcctgac gagcgccgaa aaaatggcgc ttgccggcgg aaacaatgcg gcaggcattc
     3061 cgctgagccg cgccaccaac tcccagagga ccaggagcac aatgatcggg attgcaagaa
     3121 atgcgaggtt cttgagcccg ccgatgagcc cgccgccggt ccgcacttcc tccgccgaaa
     3181 ggactggggc caatcggcga tcggaatcca gggctgccat ctcggacctc gcagaatctg
     3241 ctatttgatt gccgcctcgg cagcgtcggc aaagctcggc tcgaggacgt ctttcaaatc
     3301 tggagcagat gaaatcttac cagtcttgcg gaggaagtca cgcgtccgcg acagctcctt
     3361 ttcatcgctc gcgatgaaat tcacacccgt gaatttgtcg acctcgaaca tcatcgtctt
     3421 gatgatatcg tcctcgagtg ccgaccctgt ctctctgacc gtcttcgaga aaatgcggaa
     3481 agctccgtcg gggttggcgc ggatccattt gtgagccttc gcataggcgt tgacgaagtg
     3541 ctgcacagtg tcccggttct ccgcgatgta gttcttatcc gccacgagca gcagtggata
     3601 ggtgatttca gccatctcct ggaactgctt ggcgctgatg atctccttgc cgtttccggc
     3661 ctgaacgatg cgctgcatca tcggatccca gccgagaacg gcatcgaaag cgcccccgga
     3721 tgccatcgcc gtcggcatat cggttacgcg gacattggaa atctgaaaat cactttcgct
     3781 gagagcctgc tgctcgagga gtatcagaaa taccccgtga acgccggttc cgacctggat
     3841 gccgatccgc ttgcccttca agtcggccaa agacttcact tcgccgagag agggtacgcc
     3901 caccattttg cctgtgaacc cgcgcgaggc ggcaccgagg gcgaccagag gaatgccctt
     3961 gtcaatggcg gaaaagagca cgacatcgga gacctcggcg aactgcatcg acccggcgac
     4021 gatggcctcg actgcgagaa cgcccctgac tacaggcttg atttcgacat cgagtccttc
     4081 ctccttgaag tagccctgtt ccgcagcgac aagggaggtg acttgattga cgccgctggc
     4141 catgcccacg gtcactttgg tctgtgccga ggaaagatgc ggcaggacca gcagcccgct
     4201 gagggccagc gccagaatgg ccaatctctt catgtggctt cctcccggtg agcttggttg
     4261 tctgatgccc gcctgcagac ggaccgctcc tattctgcag gaaccatagg acaagcgcca
     4321 acctcggtca agaaattgac gatattacag tcattattga cgaactcgaa aaatatcgat
     4381 attcagatcg aatatgcgcg cctttggcag gcgtcgaatt cgagggtaaa atgcgctacg
     4441 tggcattcga agagggcggc agagccagac tgggggccgt cgtggggcat gagattatcg
     4501 atctcagccc ccttactgga gcggcggttt ccgtccgtgc cattgtcgaa agcgcagatc
     4561 tgcagtcgaa agctgctaag cacgccagca atactacggc atcgcgtctg ccgctaaaga
     4621 gttgccggct gctttcccca cttcccgatc cgggaaaatt catctgtctc ggcctcaatt
     4681 atgcggatca tgcgaaggaa ggcggtcaca gcgtgcccga ctatccgtcc ttgtttttga
     4741 gagcgacgac ctcgctcatc ggtccgcagg atcccatcgt cgcgccgcga tgctcggagc
     4801 gcctcgatta cgaggccgag ctcgtgatcg tcatcggcaa gcgctgtcgg cacgtgacgg
     4861 agcaggccgc gctcgacgtc atcttcggat acacggcttt caacgacggc agcgtacgcg
     4921 attaccagcg acgaacgacg cagtggacgg ccggcaagaa tttcgacgga accggcgcac
     4981 tggggccctg gatcgttacc gccgacgaaa tgccgcccgg agctgcgggc ctcaatattc
     5041 gctctcggct caacggcaat gtcatgcaga gctcaaatac cgatcggatg atcttcagtg
     5101 tgccgcgtac gatcgcgatc ctgtcggaga tcatgacgct ggagccgggc gacctcattg
     5161 ccaccgggac gcctgagggc gtcgggcatg cgcgtacacc gccggtctgg atgcggccgg
     5221 gggacgagat ctcggtggag atcgatggca tcggcgttct gaccaaccgc gtcgttgccg
     5281 aggccgagga ttccaccatc gccgcataag gcagaggatg atccgcgcgg aactcaacgg
     5341 tgaaccgggg cggtcgcaga ccagcctcat atacgatcgg cttcgcgacg acatcgtgca
     5401 gggcagattt gcgcccggct ccaagctcaa gatcgaggag ctgaaggcgc gctacgatgg
     5461 cggcgccacc ccgatccggg aagcgctcag cctgctggcg gcagatggcc ttgtcgagcg
     5521 gctggagcag aggggatttc gcgttgccga ggccagcgcg cgcgagttcg aggagctgct
     5581 cgcgatccgc tgctgggccg aggagcgcgc tctcaggctt tcgatcaagc atggcggcaa
     5641 tgattgggag gagggcatcg tccttgcccg gtatcgcctc gcgcactcca agcgcgtgcc
     5701 gctggaggtc agccattcca cgcatgccga ttgggaaagg aaccataaga aattccacat
     5761 gagcctggta tctgcctgcg gctcgacgac attgctgcgg ctttgcagcc agctttacga
     5821 cgagaattgt cgctatcgct acatcgcgcg gctgagcgac cacgagcggc ctgacgtcta
     5881 tggggagcat gaggaaatcg ccgaggccgt tctggcgcgc aatgcggatc tcgcagtcaa
     5941 actgatcatt gcccactaca cgcgtacggg agatctgctg cggaaaagcc ttccggccta
     6001 cctgaaagga tcggtcatta agtcggcctc ggagtcgcgc gactcctatg gcccgcaggc
     6061 caacatcttg agcgcggacg gaacgcgcaa acgtagcaga ccgaagttga tccgtgtgac
     6121 ccccaaaccg cgcaaccgaa gagcagccag ggagtgatcg gtgcagaagc tcatacccac
     6181 gaccgtcgtc ggaagctatc cgcagccgga ctggctggtt gatcgagaaa agctcaagtc
     6241 tcagtcggtg ccaagggtgc gcgtgaccga catttggcgc atagaggagc gctggctcga
     6301 ggcggctcag gacgacgcga ccgtgcttgc catccgagac atggaacgcg ctggcatcga
     6361 catcatcacc gatggggaga tccgccgcga aagctattcc aaccgcttcg tcaccgagct
     6421 cgagggcgtc gacagcagaa atccggccat cgtgacgctg cccacgggcc gccagggagc
     6481 gttgccgcgt atcgtatcga aaattcggcg gacgcatgca gtggaggttc gcgacgtcga
     6541 attcctgcgt cgcaacaccg atcggatgat caagatcacc ttgcccggcc cgttcaccat
     6601 gtcgcggcag tgccacaatg agttttacaa agatccggac gaaatggtta tggatttcgc
     6661 ggcggcgttg aatgccgagg cgaaggaact ggagaaggcg ggggcggacg ttattcagct
     6721 tgacgagccg tggctgcgcc aggaccccga agcagccaaa cgcgttgcgg tgccagccat
     6781 caatgctgca ctggagggat tgtcggccac gaccgctgtc catctgtgct tcggctacgc
     6841 ccatctcgta tcgggcaaac caggcggcta cgcgttttta gcggagcttg ccgacaccat
     6901 tgcggaccag atctcgatcg aggcggccca accgaaagtc gacctcggtg tgctcgagga
     6961 cctttcgggc aagtcgatca tgctgggcgt gatcgatctc ggtgatcccg agatagagac
     7021 gccggagaaa atagccgaac gactgcgcgc cggtctgaag cacctgccat ctgaacgcct
     7081 gattgcagct cccgattgcg ggatgaagta cctttcacga gatgtcgcgt tcggcaagct
     7141 caaggcgatg gtggacgggg cggcgatcgt gcgcgcggag cttcagggtg ccgcatgaag
     7201 cgatcgaaag caatcgacga gtcgagccag ccagcgtttg tcgtgaagtg agtttcggtg
     7261 aagcgcttaa cctatacctt cgataacggc ccatggccgg gagctacgga ccggcttctc
     7321 gatttcctca aaagccgcaa catacgcgcc accttcttcg tcgtgggcga gcggctgaat
     7381 gacccggcgg cccggcgtct cgccgagcgt gcccgtgccg agggccactg gatcggaaac
     7441 cacactctga gccacggcga ccccttgggc tttagcggcg gactcgagcg tgtcaggaag
     7501 gaaataggag aagccgaaaa acttctcggc cctctctcgc acccgcgcaa gttttttaga
     7561 ccgaacggga aaggagcatt gggtccgcat ctcctgagcc cggaggcagt cgaatacctc
     7621 actgagcatc gctacaccgt cgtcacctgg aataacgtgc cgcgagactg ggatgaaacg
     7681 cgggcgggat gggctgatcg agccgaggcg acacttgagg ctcaggactg gtcggtgctc
     7741 gtgctgcacg accggtttat cgcgccgatg atggactctc tggaacgctt tcatgatcgg
     7801 ctcctcgcac gttcggtggc gatcgtgcag gatttcccga aagcgtgtgt cccgattcgc
     7861 gatggcagat tagaaggcga tatctccggg cttgtaacgc aggatccacc ttgaggagtt
     7921 ctgccggatg aaggttctgg gcatttcagg aagcctgcgg gagcgctcct tcaacaccgc
     7981 gctcctgcga gccacccagg agcttgcgcc gcccgacatg cagatcgaaa tcttcgacct
     8041 tgcaccgatc ccgatttaca acgacgacgt tcgcctcgca ggctatccgc ccgaggtttc
     8101 aaagtttcga gatgctatcc gggcggcaga tggcgtcatc atcgcctcgc ccgaatacaa
     8161 ccgctccatt cctggcgtcc tgaaaaacgc catcgattgg gcctcgcgcc ctcccgatca
     8221 gccctttgca tgcaagccga tggccttcat gggcgtaagc aacggtgcgc tcggggcagc
     8281 cttcgccaac caccaccttc gtcagatctt cgtctacatg gacgcccgga tggtcaacgg
     8341 gccggaagtg atgataggag acgcgcggag caaattcgac gcgtcgctca acctggtgca
     8401 cgagccgacc cggaagttcg tcagcgatca cctgaagaaa cttgccgcgt tgatcaggat
     8461 gacgaagggc accgtacggc caatgccgtt gtagtgaatg cctttcgggg tcgacccctc
     8521 gagcttgatg tcgagctaga gcatctccga tttaggcaaa agcacgccaa gtcgagctcg
     8581 cgcccaatct cccttttccc ggcgccagca tgatgatgcg gggctgtgca gaaggcttgc
     8641 caagtctccg acaggcgcgt tataggcacc ttggttgccg acgaccattt cctgttgagg
     8701 caacccaccc gactcgattg gcctaacctg gaatacctcc tcgggtgggt accgcacacc
     8761 gccacctccc gaaccctccc agccagagga tgattttagg tcccacaacg ccgatttcga
     8821 cgcccggcaa atcggaaatg ccaatttgcg ttacaggtcg tgctactctg tgccaggatt
     8881 gacttgggtc tacggcgagt cgcgcacgct tgcttctgtg atatcggcac caatgactac
     8941 gacgatcaat accgtgtatg tcgggacgct ggccccgctc gggcccgatt gcgtgccgag
     9001 cgggatattc aagcgacccg ctaaaggacc ctggcacatt atgcgaacgg gaatcgtcgg
     9061 cgaccagcag ggcgatgcgc gccatcacgg cggccccgag aaagcggtgc atcactaccc
     9121 ttatgaacac catgccgcct gaatcgagga ggatccgggc atcgccgact ttctctcctc
     9181 ccctcctgca ttcggggaga acctgtcaac tttgggcatc accgaggaga ccgtccacat
     9241 cggtgacatc tatcaggtcg gcggggtcat cctgcaggtc agccagggtc gccagccgtg
     9301 ctggaagttg aatgctcggt tcaatcgccc cgatatggct tggcgtgtgc aaatgacagg
     9361 tcgaacgggc tggtactacc gggtcttgcg cgaaggctgg atcgaaacgg gtgacgaatt
     9421 tgtacttgtg gagcgt
//
LOCUS       DASVDU010000177         3267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1077272, whole genome shotgun sequence.
ACCESSION   DASVDU010000177 DASVDU010000000
VERSION     DASVDU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077272"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VF226_11145"
     CDS             complement(<1..895)
                     /locus_tag="VF226_11145"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7074587.1"
                     /translation="MPDLTADHFDEFFGVLYEAEPFPWQSRLAKRVIERRTAAWPAAI
                     ALPTAAGKTACIDIAVFALACHANRASSERTAPRRIFFVVDRRIIVDEAHRRATMLAM
                     KLRSAKRGIVKEVADRLRILAGGDEPLACFHLRGGVYRDDAWARTPIQPTVISSTVDQ
                     IGSRLLFRGYGRSSRTWSIHAGLACNDSLILLDEAHCARPFLETLQAVASYRQWADAP
                     IWSPFHTVVLSATPPEDRTDVFRDDARDRAHPVLGARLKAEKPTALCVAARSANANAL
                     VDELVERARGLVSEDSLAIGII"
     gene            complement(888..2465)
                     /gene="csb2"
                     /locus_tag="VF226_11150"
     CDS             complement(888..2465)
                     /gene="csb2"
                     /locus_tag="VF226_11150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Csb2"
                     /protein_id="HEX7074588.1"
                     /translation="MFALGIRYLNGWAMAAADGAKKEQAEWPPHPDRIFMALAAAHFE
                     TDGSVEERAALEWLETLGPPAIAVSDAEYRTVVTSYVPVNDEKVGRSRPGRATVGALK
                     DAGLAVIPEFRTRQARSFPVAIPHDPVVHLVWNDDLPPELRDSIASLSRKVISIGHSA
                     SFVQMWLESSPPTPNLVVSTKLAKFRLRITGIGRLKYLEHRFNREAVKAYADIERRVD
                     RASGKEKKMLQAELKRRFPAGAPISLRPEPGLWQGYGRPEPERRPALPHTVFDPRLVV
                     FALEGRRFDLQATLKITEAFRGTVLSRCPEPIPEWVSGHGADGKPTRAPHLALLPLPF
                     VGHEHADGRLLGMAMAIPRGVDGGEAERVLGSVIGFDDYGLPKALRLFGGEWLECALA
                     LEQREAPPMTLQPDGWVGPAKRWASVTPVVFDRHFDGKNRWEQAAEMIKTGCERIGLP
                     RPEDVILNPVSAHIGVPRSGDFPPMKRKLDGGRLHHMHAIITFMEPILGPVIVGAGRF
                     RGYGLCRPLAQGGVADA"
     gene            complement(2465..>3267)
                     /locus_tag="VF226_11155"
     CDS             complement(2465..>3267)
                     /locus_tag="VF226_11155"
                     /inference="COORDINATES: protein motif:HMM:NF021156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-U CRISPR-associated protein Cas7"
                     /protein_id="HEX7074589.1"
                     /translation="KALDYVDIRNARPLFELCPTALVFGMWDSTGPRGGLGAKFQRAM
                     VSEIVGLHAEAGVRTSSRIDPLQIMLQAGPIYATPEGDWTLDESAARKVKGKPAKVGK
                     DGRPSEVNHGNVAPSITAGGFTIGKAIQTTVISLPALRRLRFPLNGETSSEEKTDTTA
                     RTVLAALGLCAAALARVDTDLRSRCLLIPTGPIEWELLGEPGETPKRYALSPSTAIEI
                     FRQAVEEAKKAGLPWMEEPLELKPSDSLLALVRRSQELQVSQALAEEE"
BASE COUNT          631 a         1051 c          937 g          648 t
ORIGIN      
        1 ctatgatgcc tatcgcaagg ctatcctcgg agacgagtcc tcgcgcacgc tccaccagtt
       61 cgtcaacaag cgcgttcgcg tttgccgacc tcgcggcaac gcacaacgcc gttggctttt
      121 ccgccttgag cctcgcaccg agaacggggt gagcacggtc gcgggcgtca tccctgaaca
      181 catccgttcg atcttccggc ggcgtagctg ataacactac cgtgtgaaac ggtgaccaaa
      241 taggtgcatc ggcccactgg cgatagctgg caaccgcctg caacgtctcc aaaaaggggc
      301 gcgcgcaatg ggcttcgtcg agaaggatga gactatcgtt gcaggcgagg ccagcatgga
      361 tcgaccaagt acgcgacgag cgtccgtagc cccgaaagag aaggcgggat ccgatctgat
      421 ctacggtact cgagatgacg gtcggctgaa tgggcgtgcg tgcccaggca tcatcacggt
      481 aaaccccgcc tcggaggtgg aagcacgcca gaggctcgtc gccgccagca agtatccgca
      541 gtcgatccgc gacctcttta actattccac gctttgctga tcgcagtttc atcgccagca
      601 tcgtcgcgcg ccgatgggcc tcatccacga tgatacgtcg gtccaccacg aaaaagattc
      661 gccgtggagc tgtgcgctcg ctgctggcgc ggttcgcgtg gcaggcaagc gcgaaaacgg
      721 caatgtcgat acaggccgtc ttgcctgcgg cagtagggag ggcgatggcg gccggccaag
      781 ccgcggttct gcgctcgatt acgcgcttgg cgagccttga ttgccaagga aagggctccg
      841 cttcatagag caccccgaag aactcatcaa aatggtctgc cgtgaggtca ggcatcggca
      901 acccctccct gcgccagcgg tcgacacagg ccgtacccac ggaaccgtcc cgcgcctacg
      961 atcaccggcc ccaatatagg ctccatgaaa gttatgatgg cgtgcatatg gtgtagcctc
     1021 ccaccgtcca gcttccgttt cattggcgga aagtctccgc tccgcgggac gccaatgtgt
     1081 gcagacaccg ggttgagaat cacgtcctcg gggcgcggga gcccgatacg ctcgcacccg
     1141 gtctttatca tctctgccgc ctgctcccag cggttcttgc catcgaagtg acgatcgaaa
     1201 accacgggcg ttacgcttgc ccaacgcttt gccggtccaa cccaaccatc gggctgaagc
     1261 gtcattggcg gcgcttcccg ctgttccagc gcgagagcgc actcgagcca ttcgccgcca
     1321 aatagccgca gcgcctttgg caggccgtaa tcatcgaaac caatcacgct gccaagcacc
     1381 cgttctgctt cgcccccgtc cacgcctcgc ggaatcgcca tggccatgcc gagcagccgg
     1441 ccgtcggcat gctcgtggcc cacaaacggc aacggtagta acgcaagatg cggcgcacgc
     1501 gtcggtttac catcggcacc atgtccactt acccattccg gaatcggctc cggacacctg
     1561 ctgagcaccg ttcctcggaa tgcttcagta atcttgagcg tcgcctgcag atcgaagcgt
     1621 cggccctcca gagcaaaaac aaccagccgc gggtcgaaca cggtatgcgg caaggccgga
     1681 cgccgctccg gctcgggtcg gccgtagcct tgccataggc cgggctcggg tcggagactg
     1741 attggagcgc cggccggaaa gcgtcgtttc aattcggcct gcaacatttt cttttccttt
     1801 ccgctcgcac ggtcaactcg ccgttcgata tcggcgtacg ctttcacggc ttccctattg
     1861 aagcgatgct cgagatactt gagccgccct ataccggtga tacggagacg aaatttagca
     1921 agcttcgtac tcaccacgag gttcggtgta gggggggagc tttccagcca catctggaca
     1981 aatgacgccg agtggccaat cgatatcact ttgcgggaca aggatgcaat cgaatctcgg
     2041 agttccggag gcagatcgtc gttccaaacc aagtgcacca cgggatcgtg cggaatagcg
     2101 actggaaacg accgcgcctg tcgagtgcgg aactccggaa ttaccgcgag accggcgtcc
     2161 tttagtgcac ccactgttgc ccggccaggt ctcgatctgc cgaccttctc gtcgttaacc
     2221 gggacgtagc tcgtgacgac agtccgatat tcagcgtctg atactgcgat ggccggagga
     2281 ccaagcgttt cgagccactc gagcgctgct cgttcctcca cgctaccatc ggtttcgaag
     2341 tgggccgcgg ccagcgccat gaatatgcga tcggggtgtg gcggccactc ggcctgttcc
     2401 ttcttcgcac cgtcggccgc cgccatcgcc catccattga ggtagcggat accaagcgcg
     2461 aacattactc ctcctcagca agggcctggc tgacctgcag ctcctggctg cgccgtacca
     2521 gcgcgagcaa gctatcggac ggcttgagct caagtggctc ttccatccac ggcaatcccg
     2581 ctttttttgc ttcctcgacg gcctggcgaa aaatttctat tgcggtactc ggcgagagcg
     2641 cgtagcgctt cggtgtttcg cctggctcac cgagcaactc ccactctatc ggtcccgtgg
     2701 gtatcagcag gcagcgggaa cgcaggtcgg tgtctacccg cgccagagcc gccgcgcaga
     2761 gcccgagcgc tgcaagtacg gtgcgggctg tagtatcggt cttctcttcc gaacttgttt
     2821 caccgttcaa tggaaaacgt agccggcgca aagcgggtag cgagatcacc gtcgtttgta
     2881 ttgccttccc aatcgtaaat ccccctgctg taatagacgg tgccacgttg ccgtgattca
     2941 cctcggaagg tctcccgtct ttgcccactt tagcgggctt gcctttgacc tttctggcag
     3001 cactttcgtc aagcgtccaa tcgccttccg gcgtggcgta tatcggtccc gcctggagca
     3061 tgatctgcaa tgggtcgatc cgacttgacg tcctgacacc cgcctcggca tgaagcccga
     3121 cgatttccga aaccatcgcc cgctggaact ttgcgccaag cccgccgcgc gggcccgtcg
     3181 agtcccacat gccgaacacg agcgctgttg gacatagctc gaaaagcggg cgcgcgttgc
     3241 ggatatctac gtaatcgagc gctttgc
//
LOCUS       DASVDU010000178        47415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1162826, whole genome shotgun sequence.
ACCESSION   DASVDU010000178 DASVDU010000000
VERSION     DASVDU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 47415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47415
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162826"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VF226_11160"
     CDS             <1..259
                     /locus_tag="VF226_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEX7074590.1"
                     /translation="EELHVGLKEAIDAAGVDLVFACGPNMGRLFATLEPGRQAAWTET
                     SEGLATPLLGAVRAGDAVMIKGSLGSRMAPLAEALKNRFAT"
     gene            332..1414
                     /gene="mraY"
                     /locus_tag="VF226_11165"
     CDS             332..1414
                     /gene="mraY"
                     /locus_tag="VF226_11165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7074591.1"
                     /translation="MLYELVNFSDYINALNVFRYITFRTGGAIMTALLFVFLFGPIII
                     DLLRLKQGRGQPIRDDGPQSHLAKRGTPTMGGLMILSGFTVATLLWANLSNPYVWVVL
                     GVTLAFGAIGFCDDYMKVTKSTVAGFSGRLRFAIELGIAGIATGALMWLSVPELTSAL
                     TVPFFKNIVFDLGPFFILLGVIVIAGAGNAVNLTDGLDGLAIVPVMIAAATFGLIAYL
                     SGNINFARYLQIHYVPGTGELAILCGALIGAGLGFLWFNAPPAMIFMGDTGSLALGGA
                     LGTIAVATKHEFVLAIVGGLFVIETLSVIIQVVSFKLTGKRVFRMAPLHHHFEQKGWK
                     ESTVVVRFWIISVVLALIGLATLKLR"
     gene            1461..2870
                     /gene="murD"
                     /locus_tag="VF226_11170"
     CDS             1461..2870
                     /gene="murD"
                     /locus_tag="VF226_11170"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7074592.1"
                     /translation="MIRVTTFAGKTVALVGLGASGRATANALLAGGAEVAAWDDNAEA
                     RTAASQAGIPVADLSAMDWRGFAALVLAPGIPLTHPEPHWAVARARAAGVEIIGDIEL
                     FCRERASRSPDAPFVAITGTNGKSTTTALLAHLLRAAGRDVAMGGNIGTPILALPEPA
                     SGRVHVIEMSSFQIDLTPSLAPTVGVLLNLTPDHLDRHGTMEHYAAIKERLVTASCHP
                     IIGVDDDYCQRIADRLRTARNGDLDALSVLRPLPGGWFADGSRLHSDADWAGISAVFA
                     DLNGIGTLRGRHNAQNALAACIAALRLGVLPDQLQPALATFPGLPHRMEEVGRAGRVL
                     FINDSKATNADSTEKALAAFPRDIYWIVGGRPKEGGIEPLRPYFPRIAKAYLIGESTE
                     SFAATLGAGVPFERSGTLDIAVRTAAREAAQSAGAEPVVLLSPACASYDQFKNFEQRG
                     NVFRELVAALPGVTVPGRP"
     gene            2867..4048
                     /locus_tag="VF226_11175"
     CDS             2867..4048
                     /locus_tag="VF226_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEX7074593.1"
                     /translation="MKISRTDRSLVAEWWFTVDRVLLTAILILAGSGLVLSLAASPAV
                     ALKKGFSAFHFVERHAAFAGLGVLIMLAVSMTQPRLIRRLALILFVGGIVLMVGVLAF
                     GEEINGARRWLRLAGFSLQPSEFAKPAFIVLTAWAFGEAQKQVDMPARSIAIALLAVF
                     DLLLLRQPDLGQTFLVSAIWATLFVLSGQPIIWAGIFGVAGVVGLTGAYFTFDYVRLR
                     VDRFLSPGGDEYSQTARALQSFVEGGFFGRGPGEGSIKTALPDAHTDFIFAVIAEEYG
                     AIACLALVALIAIIVLRAYVRTFTETDPFTRLAVAGLSLLFGLQAAINMAVNVGLLPA
                     KGMTLPFISAGGSSLVSMFLTMGMILGLTRRRPDVLRLRRPHSALKNEDSLGRLGSAA
                     Q"
     gene            4045..5184
                     /gene="murG"
                     /locus_tag="VF226_11180"
     CDS             4045..5184
                     /gene="murG"
                     /locus_tag="VF226_11180"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX7074594.1"
                     /translation="MNAPSVILAAGGTGGHLFPALALAQELGRRAIVVDLMTDTRGDR
                     YGSDFPARHIYRVPSATIGKGHGFLDKAKTALTLGRGILSAYRQLGSIKPGAVIGFGG
                     YPSFPPLVAAFLRHIPTAIHEQNSVLGRANRMLASRVTAIATSFSALHHLDAALEAKV
                     RLTGNPVRDAVIDRSSRAYAPPASDGTFTLLVFGGSQGARFFSDTVPAAIALLKPETR
                     QRLRIVQQCREEDLGRVRQAYAALEVDMELASFFRDLPERMAAAHLVIGRAGASSVAE
                     LTVMGRPSILVPLPHAIDNDQLRNATQLAKSGGAICIEQKDLTPELLAREVERLSANP
                     EALAAMAAAAKAQGRPDAVVRLADLIEELMGLRQPPRQPDERGKH"
     gene            5195..6631
                     /gene="murC"
                     /locus_tag="VF226_11185"
     CDS             5195..6631
                     /gene="murC"
                     /locus_tag="VF226_11185"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX7074595.1"
                     /translation="MEIPRGIGTIHILGIGGIGMSAIAEILHAKGFSVQGSDQKESAN
                     VKRLRGQGIRVFVGHQAENLADAKYVVISSAVKDGNPELEAARARGLPIIRRAEMLAE
                     LMRLYATVSVTGTHGKTTTTSLVAHIFKEAGLDPTVITGGIINDWGSNARLGASPWMI
                     VEADESDGTFVKIPTQIGVVTNIDPEHLDYFKTVENMHREFEAFYRNIPFYGLMVTCT
                     DHPVVRDMVDRLHLRQDGRRLVTYGTQADADMVLSEVRFDGLDTIFDAELRERVSGGA
                     RSIRGWRVPVPGQHNALNALAAIAVAAEAGIPDDVIRQALASFSGVKRRFQLTGTWNG
                     VFIFDDYGHHPAEISAVLKAARAGARGRVIAVIEPHRYTRVRDLFQDFCASLQAADSV
                     IVTPLYSAGEAPIDGIDHVALAEGIRCTGHASVFPVDDEQDVVPVLRRIAQPGDMVIC
                     LGAGNSTELAHALPELLGGEARLAGAAS"
     gene            6628..7581
                     /gene="murB"
                     /locus_tag="VF226_11190"
     CDS             6628..7581
                     /gene="murB"
                     /locus_tag="VF226_11190"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7074596.1"
                     /translation="MSFPDLTGDLTARMPDLRGRLVANFDLSSITWFRVGGPAQLLFT
                     PADEADLAYFLSRAPADLPVTVIGLGSNLLVRDGGVPGVVIRLGRGFSEVKVEPGHRL
                     RAGTAVPDVKVARAAADAGIAGLAFYRGIPGSIGGALRMNGGAHGTETKDVLVEARAV
                     DRKGNIHVLSLADMGYSYRHCSVPGDWIFTEATYQGRPGKAEDILKQMEEVAQYREEN
                     QPTRERTGGSTFKNPPGHSAWKLIDAAGCRGLRIGGAKVSEMHCNFLINEGQATAEDI
                     ERLGETVRARVKANSGVTLQWEIVRLGLPLDGRPTGEALAA"
     gene            7730..8668
                     /locus_tag="VF226_11195"
     CDS             7730..8668
                     /locus_tag="VF226_11195"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7074597.1"
                     /translation="MSADSAGRKYEHVAVLMGGWSAEREVSLSSGNACAAALEAAGYR
                     VTKIDVDRDIARTLKELAPDVCFNALHGRWGEDGCIQGLLEVLGIPYTHSGVRASALA
                     INKEQTKRVVSGLGVPTSEHRIVSRREAVRTHAMEPPYVAKPICEGSSVGVVIVREGA
                     NRPPEAIATVPAIEDEIMIERFIAGRELTCAVIGGQATDIIEIVPAARLTFYDYEAKY
                     APGGSRHILPADIPRDVYEAVQRHSVTVHNALGCRGVSRSDFRYDPATGQLAFLEINT
                     QPGMTGTSLVPEMAAHAGMSFQQLVSWLVEDASCNR"
     gene            8854..9687
                     /locus_tag="VF226_11200"
     CDS             8854..9687
                     /locus_tag="VF226_11200"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEX7074598.1"
                     /translation="MKLSAAPMPKRPRPRRQSAVRLTAWTTAIAGAALFIALTQWGAN
                     ARQARPVVEEADRLAELAGLGVQQVYLTGHRMTSDRDIFDALDLTQARSLLRFDSVAA
                     RERIERLPWIKTAAITRIFPDSISIEVVERKAFAVWEESGKDLLIDATGRVLGLAPPS
                     MQAGLPRFVGRGANEKAAALLSLLEHQPYVRARLKEAVRVAGRRWTLRLAGGLEIHLP
                     AGREAEALARLSSSVAASRLLDANEYAAIDLRPADRIIIRPVHDGERKTASSQHVQPS
                     G"
     gene            9710..10975
                     /gene="ftsA"
                     /locus_tag="VF226_11205"
     CDS             9710..10975
                     /gene="ftsA"
                     /locus_tag="VF226_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX7074599.1"
                     /translation="MPFDSSSGDVLGLIDIGTNKICCLIMVRDRGYRFGRGARSYRVA
                     GIGHQRSRGVKAGVIVDLDEAEQAVRAAVAQAERMAGLRIEEVVVGAACGRMRSSHFA
                     AHADIESGYIRESDLRRLKAAGRAYAERDGRLLLYLDQLNWRIDGAPGIRDPIGMAGR
                     RLSADLHAITADDAPLRNLMLLIERCYLTLSRAIPSPLASALAVTSEEERRLGITCLD
                     IGGGMTSISVFVDGRFLFADSVPVGGNHMTYDIARQLRTPLAEAERIKALYGTLVAAR
                     SDEHEVITYPLAGEEEQTLHHTTKARLRSILQPRMEGLLELVRERLQRSNIKSYAGER
                     VVLTGGTSQLIGIGEFAANTLGRPVRVARPQPIGGMSASSCSPAFATVIGLVAAAEVS
                     QSCWASFPEYPSSQESYFGRMGQWLRQSF"
     gene            11072..12814
                     /gene="ftsZ"
                     /locus_tag="VF226_11210"
     CDS             11072..12814
                     /gene="ftsZ"
                     /locus_tag="VF226_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX7074600.1"
                     /translation="MSISLQLPKVTDLKPRIAVIGVGGAGGNAVNNMIASGLSGVSFI
                     VANTDAQALAASSAEHRIQLGTNLTEGLGAGSKPEIGEAAAEEAVDEIRAQISGSHMV
                     FIAAGMGGGTGTGAATVIARIAKELDILTVGVVTKPFHFEGSRRMRIAEAGIAELRKI
                     VDTLIVIPNQNLFRVANEKTTFAEAFVLADQVLYSGIACIVDLIVKEGLINLDFADVR
                     TIMSGMGTAMMGTGEATGDQRAIMAAEEAISNPLLDDISLRGAKGLLLSIIGGRDLTL
                     FEVDEAASRVRQEVDPEANIIVGATFDESMGDRVRVSIVASGMPPEETKPAAPATGAY
                     RSSGRQAPPPQHAAPHPAPQAPMDRPAAAAPGVSSGPSPKGSDNFMDALSRAIEEKPG
                     QTSGEPKPKDVWHGPGDVVIEEGLPPIGLGPSTAPPPPSRGAGAQAGARLPEPFTPGP
                     PVEVKRQHRRMPTVEDFPPVAQREYRAKAAWYETGPSTPPSSRGYAPQKEPAKKASFF
                     SRITGLGWRTEQPAERHARENSHNPGRAHDGMSSDHASTPGRAPHEGDRPDDRLGSQG
                     ADFPMFFQKDRKRQ"
     gene            13072..14010
                     /gene="lpxC"
                     /locus_tag="VF226_11215"
     CDS             13072..14010
                     /gene="lpxC"
                     /locus_tag="VF226_11215"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7074601.1"
                     /translation="MSKRFIGARQTTIAREVELQGKGVHSGAPVSVTLHPAGPNTGLK
                     FLVNKRGRIIAEIPGEVGYVKNLTLCTVIGDDSGVTVGTVEHLLAALRGLSVDNCYIE
                     IDSKEVPIMDGSAAPFVEAIDQAGIRELPEPRKYIKVLKPIRVEEGACWGELTPHSGF
                     HLDIEINFDTPLIGQQRLAFDINPGVFRHELSIARTFGFMHDVEKLWKAGLALGASLD
                     NTIAIGEDRILNREGLRCPMEFVRHKMLDAVGDLALSGYPLLGAYRSVRGGHRLNASV
                     LKALFADASAWTLVQAPRVREPFVRLPAAMAVAAGE"
     gene            14314..15159
                     /locus_tag="VF226_11220"
     CDS             14314..15159
                     /locus_tag="VF226_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7074602.1"
                     /translation="MHGKRRAGLVSALLALCVVMSGCASSDDAAKLLNPDPPEKMYAE
                     ADQLMSRGKYEAAAQKFEDLDRDHPYSQEARRAMVMAAYAYYRAGKTQEAIATARRYT
                     TMHPGTKEAPLAHHIIISAYFDEMVGPNRDQTNARKALAELKTLKARYPDSPYARDAD
                     NRIRIAEDSIAAHEMEVGRYYLKRKNYMAAINRFKTVVTEYQTTAHVEEALERLVEAY
                     MALGIKHEAQTAAAVLGHNFPSSPWYKDAYALLQSDGLAPREDTGSWLSRAWQNFKLP
                     KLTSG"
     gene            complement(15494..15913)
                     /locus_tag="VF226_11225"
     CDS             complement(15494..15913)
                     /locus_tag="VF226_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074603.1"
                     /translation="MRSRATIAFLCLAAALTFAEPAPAQGLRYIQMREAVAACRADYF
                     RLCPGVRPGAGRVLVCLNAQAEKLSQPCFQALAERGLAFAAVLRLCRPDFERLCPGVR
                     PGMGRALVCLLDNAEQVSQGCRTALSAHGFSVDDDVR"
     gene            complement(16036..16545)
                     /locus_tag="VF226_11230"
     CDS             complement(16036..16545)
                     /locus_tag="VF226_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074604.1"
                     /translation="MPMLSRLTAAAFVALSVLATRAAPAAAWVAGPPGARVLAWVCYA
                     DYVRYCRGVPTGTGEALRCLAEQHHALSRPCGQALKIVATIDACAGDFHEYCPGVVPG
                     AGRGAACLEGNFDRLSRECVRALSATFPYHFEQAYVEHHRRPGFPRDFGSADDFTVNP
                     PDLDDAPLK"
     gene            complement(17032..17328)
                     /locus_tag="VF226_11235"
     CDS             complement(17032..17328)
                     /locus_tag="VF226_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074605.1"
                     /translation="MKAYLLAGLALVVASGPALAGTPYVDKREHRQAHRIYNGIKSGE
                     LTYGEAARLIRGQHRVRRLERQFKSDGVVTPAERAILHHRLNVQNVRIYKNKHD"
     gene            17562..18287
                     /locus_tag="VF226_11240"
     CDS             17562..18287
                     /locus_tag="VF226_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7074606.1"
                     /translation="MIQEPQALIAIVEDDEEILRLVSSLLSQEGYAVEAAENGAALDA
                     VLSRTRPDLIILDLMLPGEDGLSICRRLRANNGSIPILMLTAKGDPIDRVVGLELGAD
                     DYLTKPFNPRELLARVRAILRRSRGMTEAEANRRYAFADFVIDLDARTLSTAAGEPVP
                     LTTAEFDLLACFVQRPRRVLSREQLLDWTRGRDADPFDRAIDMTISRLRKKIDAAAPG
                     VNLISTVRNNGYLFVANVKHLSP"
     gene            18284..19648
                     /locus_tag="VF226_11245"
     CDS             18284..19648
                     /locus_tag="VF226_11245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7074607.1"
                     /translation="MIRLGFTARLIAIFATSLVALQLLAVVAYVMQRSRATEGSLRLP
                     LPDQAAALVELLETMPKEQWPTLLRAANSTDLSVRILDVPPDDREPEWYEAPVVEFIL
                     RRYLSVLGDREVRVRVEPSSEELVGPLKALAWVSPGAVEIEVRLKTGETLIVSAGGML
                     SLSVLGLPPGFWAGVLGLGVAALALFLLRREARPLRELAAAVDQAPVGEDAHVIPDAP
                     RSAPEIRALIGAFNRLTERIASLLRSRMALVGGISHDLRTYVTRLRLRIDLIPDGTEK
                     EKAIRDLDDMTQLLDDALTAFKAGSVAREEELVDLADILVREAEDRRLAGACVTLDLP
                     EPVRAAQVLGDALALRRCVSNLIDNAIQYGLEARVSAVLRNQRVVVMIDDRGPGIRTE
                     DRDTVMEPFVRLETSRNRRTGGAGLGLAIARGVAEAHGGHLTLGDAPGGGTRAIIELP
                     VFQD"
     gene            complement(19760..20044)
                     /locus_tag="VF226_11250"
     CDS             complement(19760..20044)
                     /locus_tag="VF226_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX7074608.1"
                     /translation="MTTLQASAQQQLRQLVEQLERLEEEKKAIADDIRDKFAEAKALG
                     FDVKVLRQVLRLRKKSKADRQEEEAVLETYLHALGMLDMEPEAPQVMAAE"
     gene            complement(20226..20531)
                     /locus_tag="VF226_11255"
     CDS             complement(20226..20531)
                     /locus_tag="VF226_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX7074609.1"
                     /translation="MQIDDKTRTELEAAAFRRLVAHLRERTDVQNIDLMNLAGFCRNC
                     LANWFQEAANAAGHPMDKNEAREFIYGMPYEEWRARYQKEATPEQKAAFEKSHPHSH"
     gene            complement(20581..21396)
                     /locus_tag="VF226_11260"
     CDS             complement(20581..21396)
                     /locus_tag="VF226_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX7074610.1"
                     /translation="MKTQKQWIEPVCSDSFVILPGPADAGLLLLCDHAGNAFPPEYGT
                     LGLPESQLKRHIAYDIGAAEVTRLMSEALAVPAVMTRYSRLLIDPNRGEDDPTLIMRL
                     SDGAIVPGNRVITAEEREKRVRLYYRPYHEAIDRVLDRCVGSGVAPAILSVHSFTESW
                     KGVSRPWHAGVLWGDHDDRLAEPLLEALCAENDLIIGANEPYVGQLEGDTLWKHGTMR
                     GLAHAIIEVRQDLIRDPKGQAAWAERLVRIMRGLLARPDLQLNVMKPPLAQAS"
     gene            21521..22825
                     /locus_tag="VF226_11265"
     CDS             21521..22825
                     /locus_tag="VF226_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX7074611.1"
                     /translation="MREDQKHSTEEQRRTLLELYDAAVKRAHPSRCLPGALPEPPPNG
                     RIIIAGAGKASAAMAVAAEQFYRERGLLDRIAGFVTTAHGYALPTERIEIVEAGHPVP
                     DAASAAAADRALALAQQAGAEDIVLVLLSGGASALWSAPVDGVSLDSKQALTRDLLKS
                     GAPIHDLNCVRKHLSRIKGGKLAAAAHPARVVTLAISDVPGDEPATIGSGPTVGDPTT
                     LADARSVLHRFDITPAPEIAAALDNSANETPKPGAAVLASSEFKLIAAPRASLEAAAE
                     VARHKGYRVENLGDALEGEARDVARSHATLAREALNNGERVAILSGGELTVTVRGSGS
                     GGPNQEYALGLVLALDGAQGIAALAADTDGIDGGGGRPEDPAGALVFPDTKARASALN
                     LDAATFLANNDSTTYFRELGDLVLCGPTQTNVNDFRVILVDP"
     gene            22973..23356
                     /locus_tag="VF226_11270"
     CDS             22973..23356
                     /locus_tag="VF226_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEX7074612.1"
                     /translation="MTQPAHADLKLCNASQSRVGIAIGYQDAKGWATEGWWNIGAQTC
                     ETLLKGAVPSRFIYVYAVDYDRGGEWAGTNYMCTQDKSFAIRDVTDCQRRGYRRTGFF
                     EVDTGDAKDWTIRLSNPEDAGAQAK"
     gene            23353..24798
                     /gene="pyk"
                     /locus_tag="VF226_11275"
     CDS             23353..24798
                     /gene="pyk"
                     /locus_tag="VF226_11275"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7074613.1"
                     /translation="MRRNRRVKIVATLGPASSDPEMLERLFESGVDVFRINMSHTTHE
                     AARKLHATVRNAEVKFKRPIGILCDLQGPKFRIGEIEGGRVFLSEGSLFRFDTNEDVG
                     SAERVFLPHPQIFEAVEPGHTLLLDDGKLRMRVVDRSVREITAEVVVGGALASRKGIA
                     TPDSVLPINPITEKDRADLDYALRLGVDWIALSFVQRGEDLVLARRLIGQAASIMAKI
                     EKPSAIADLDEIIANADGFMVARGDLGVEMPIERVPGLQKEITRKARAAGKPVVVATQ
                     MLESMISSPVPTRAEVSDVATAVFDGADAIMLSAESAVGQFPIEAVRMMDRIAIQVEN
                     DPGYDDLVHATPIVPLPTGADAIAAAVEKIARTMRLACIICYTATGSTALRVARERPG
                     LPVIGLTPVLATGRKLAIVWGIHSVLTGDPANLADMVRKACKIAFEDQFASPGDGVII
                     TAGVPLGTPGTTNMVRVAYLNESGAPMAEQV"
     gene            complement(24842..25534)
                     /locus_tag="VF226_11280"
     CDS             complement(24842..25534)
                     /locus_tag="VF226_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7074614.1"
                     /translation="MLAHRLIAMAAGACLLGAVAVFPSVSRAQAPGAPETSPEIPNLP
                     EPDDELQGDAADAPELREKLPDSAVERAKMLDNLYALLATAENEEAAERTSKAIARLW
                     LNSGSDTVTVLMARAAKAMEDKNPRLALRMLDAVVEQAPDYAEGWSQRAHVLYLEGEV
                     HRALGDLRRALALDPNHFRALSALGHILREYGAKEGALAAYRKLLEVNPFAEGAQKAV
                     EELEREVKGQGI"
     gene            complement(25602..25727)
                     /gene="ykgO"
                     /locus_tag="VF226_11285"
     CDS             complement(25602..25727)
                     /gene="ykgO"
                     /locus_tag="VF226_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEX7074615.1"
                     /translation="MKVKNSLKSLRSRHRDNRLVRRKGRVFIINKSQRRFKARQG"
     gene            complement(25921..27156)
                     /locus_tag="VF226_11290"
     CDS             complement(25921..27156)
                     /locus_tag="VF226_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX7074616.1"
                     /translation="MVELAVNAERLWSTLMASAEIGRTERGGLRRLALSDEDKAMRDL
                     FAQWVNEDGYALRIDQLGNMFVRREGTEPQLPPVLVGSHLDTQAKGGRFDGILGVLAG
                     LEILRTLDEARIQTRRAIEVVNWTNEEGARFQPPMMCSLVFSGAESVAWAHDRRDKDG
                     LSFGSELARIGYLGEAPVGGRALDSYFELHIEQGPKLDEAGVPLGVVTGGYQMIGMRI
                     VVRGETSHVGPTPMHLRSNALVGASHLVVAIDDIGWRYAPEDGKATAARLDLAPNLPG
                     ILSDEAQLYVDFRHPSPARLKDMEREVKAAVAESARRSRTEIEIAEQWGFGGLEFDPR
                     LADLVRTTARQLDVPFMDIRSQAGHDAYHVANVCPTVMLFTPCKGGISHNEREDIDLE
                     ATIPGVNVLLKAVLARANS"
     gene            27371..28099
                     /locus_tag="VF226_11295"
     CDS             27371..28099
                     /locus_tag="VF226_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX7074617.1"
                     /translation="MNLQDFTTLTFDCYGTLIDWESGMVEALKPLTSRVSRPLSRDDI
                     LEAHARQESSQQSWTPAKRYSELLAVVYKRLAEEWGVAVTWEECQAYGNSIGNWPAFP
                     DTAEALRYLKQHYKLVILSNVDNASFALSNKKLGVDFDAIYTAEDIGSYKPSLRNFEY
                     MIEKLEALGVAKSQILHTAESLFHDHLPANQVGLRSCWIHRRHEAGGFGATMKPETVP
                     KCDFRFTSLAAFVEAHKQATGSSA"
     gene            complement(28160..29965)
                     /locus_tag="VF226_11300"
     CDS             complement(28160..29965)
                     /locus_tag="VF226_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7074618.1"
                     /translation="MKRETSGQILSAVFVDYDNIYLSLKRRSEEAAKRFAKDASVWLA
                     EIESGRLITPTNKLPSNVQRRIVLNRCYGNPVPRRNQNDNSTDMNSFPFVRHHFLRAG
                     FEVVDCPPLTAQLKNSSDIRMVMDVRDYLHHETYFDEFIILSGDADFTPVLHRLRAHA
                     RRTVVFANDFTAAPYVAIADGEVREADLMNVILGSSVRADDHTPGAGGDLRREIMAEI
                     TGAVRSSGSPVPLEALAERATRALGHDRTVGTNWAGAGSFRDLLLQSLPEGIRLSEQP
                     PYVVYDATRKSQPEEALLSSRAEQRNERPAALSPQRQPRSAAVAAAAALTQALDPLAA
                     AKRIVSSQPQSSQPSYRPSSTPLSEQLRESSVEITPAAPQRGLSAPGPSTTLPAARTA
                     DTATALQQSIARIHEACQAPPLSPPEYRALFEMLANEINSSGLQGAQTITNVANRARE
                     LGLDIRRDDIRFALDVVSEADPWFEQGASANLFAGRFRNFVVARCRSHGLKLTPDEID
                     LIDAWFTGASSAPAAARPSSQTSSQSLTHTSTQQPASIAREAQSDRWWGADERRATQG
                     DQAGSQRASSQESAPAGELPDGDEFPRIVRTRLRG"
     gene            complement(30290..31060)
                     /gene="gloB"
                     /locus_tag="VF226_11305"
     CDS             complement(30290..31060)
                     /gene="gloB"
                     /locus_tag="VF226_11305"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX7074619.1"
                     /translation="MAKLEIHQFPCLSDNFGVLIRDADADVVASIDAPDAEAVKRALA
                     EKGWRLTHILTTHYHADHTGGNLALKAETGCTIIGPRAEAAKIPGLDQAVGEGDVFPF
                     GSFQVHVFDCPGHTAGHIIYWIPAADVAFVGDVLFAIGCGRVNEAPYEVMWQSLEKVM
                     ALPPSTQVYFGHEYTLSNAKFALTIEPENAALQQRMKDVEKMRAEGKPTSPTRIDLEL
                     ATNPFLRPNSPEIRKRLGMEGEPDWKVFAEIRERKNRA"
     gene            31200..32048
                     /locus_tag="VF226_11310"
     CDS             31200..32048
                     /locus_tag="VF226_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7074620.1"
                     /translation="MNCDVAELRNFYNRPLGSMVRRLLAHRIRARWRRLQGETLIGLG
                     FATPYLGSFRGEALRIGALMPAAQGALVWPDIGAKMSALVDEEHLPLPDSSVDRLLAV
                     HCLETADRPRTMLREIWRVLAPQGRLMMVVPNRRGVWARLDRTPFGQGRPYSRTQLED
                     LLSEAMFAPLEWSQALYVPPVESKMVIRSATAFERVGARLSRGFGGVILVEARKELTV
                     PIIKKAPARVLGRLVTAPGGANPRSETEEKRIAGPDQIADRRFRRNTAFSPKMFCTVS
                     GTRGES"
     gene            complement(31961..32602)
                     /gene="metW"
                     /locus_tag="VF226_11315"
     CDS             complement(31961..32602)
                     /gene="metW"
                     /locus_tag="VF226_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX7074621.1"
                     /translation="MLRSQQPLQVTARVDHLLIAEMVTEGARVLDVGCGDGALLQLLV
                     DTKGVDGRGIEIERERVNACVARGLSVIQGDADQDLDDYPDDAFDYAILSLTIQATLR
                     PKAVLQNLLRIGRRAIVSFPNFGHWQVRTRLLFTGRMPQTENLPEPWYESPNAHLCTI
                     RDFAELCTKVNATVERSAAFNAGGRQLSPRVPLTVQNIFGEKAVFLLKRRSAI"
     gene            complement(32627..33823)
                     /locus_tag="VF226_11320"
     CDS             complement(32627..33823)
                     /locus_tag="VF226_11320"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX7074622.1"
                     /translation="MNTVNPQEFDRGIEPRVSEAQRPSSKLLEFNEPLLLDCGQALVP
                     FAQAYQTYGELNADKSNAILVCHALTGDQHVANRHPVTGKPGWWETMVGPGKPIDTNR
                     FFVICPNIIGGCMGSTGPASINPKTGKPYGLSFPVITIGDMVDAQVRLIDRLGIDQLF
                     CVIGGSMGGMQVLEWAARHSERVFTAVPIATAAWHSPQNIAFNEVGRQAVMADPDWCG
                     GRYLELGRVPRRGLAVARMAAHITYLSEEALHRKFGRNLQDRTDRSFSFSADFQVESY
                     LRHQGISFVDRFDANSYLYITRAMDYFDLAAEHGDVLANAFKGTKTRFCVVSFTSDWL
                     YPTRESKEIVQALNAVAANVSFVEIESDKGHDAFLLDEPELFATVRGFINSAAAKRGI
                     SPARAG"
     gene            34016..35122
                     /gene="hisC"
                     /locus_tag="VF226_11325"
     CDS             34016..35122
                     /gene="hisC"
                     /locus_tag="VF226_11325"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX7074623.1"
                     /translation="MASGMPSPKRGVMEIEAYVPGESKVPGGLEPIKLSSNENPLGAS
                     PAAVAAFKAAADSLERYPDGSATELREAIARVHGLNPDRIVCGAGSDELLNLLAHAYL
                     EPGDETIFTEHGFLVYRIATLGCGAKPVVAPEKDLHTDVDAILARVTAKTKIVFIANP
                     NNPTGTYIPFDEIRRLRQGLPDHVLLVLDAAYSEYVRRNDYEAGIELVATTGNTVMTR
                     TFSKIHGLAALRLGWAYCPEAVADVLNRIRGPFNVSAPAIAAGVAALGDRGHVETSVA
                     HNEKWLPWLTAELEKLGLAVTPSVANFLLVHFPKERGRDADAANAFLQSRAIIVRRMG
                     AYGLPGALRITIGTESENRAVVEALTAFMAGTAR"
     gene            35119..36069
                     /locus_tag="VF226_11330"
     CDS             35119..36069
                     /locus_tag="VF226_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEX7074624.1"
                     /translation="MSGPLFQRVALIGIGLIGSSISHALRRRGLVETIVGNAKTEATR
                     ATALRLGLVSAAYESPADAVKEADLVILCVPVGAYGAIAQEIAPHLAPGAILTDVGSV
                     KSAVIRDVGPHVPAGVHFIPGHPIAGTEHSGPEAGFAELFDNRWCVLTPPNGVDAEAL
                     QKLRRLWEGMGSNVEIMTAEHHDLVLAITSHVPHLIAYNIVNTAAHLERVTDSEVIKF
                     SAGGFRDFTRIASSDPTMWRDVFLNNKEAVLEMLGRFTEDLTALQRAIRYGEGDTLFE
                     LFSAARAIRRGIIEAGQDTPAPDFGRRQAKAALGGGGSQS"
     gene            36066..36785
                     /locus_tag="VF226_11335"
     CDS             36066..36785
                     /locus_tag="VF226_11335"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX7074625.1"
                     /translation="MKEAGSSRKTHDLWVFGYGSLMWRPGFQFEEVRRATLVGYRRCF
                     CLYSTHHRGNEERPGLVLGLDRGGTCQGLAFRVPAECRLETLEYLAAREQVSGAYRDT
                     LVPVTLEGEPRQDVFALAYIVERRHPSYAGRLSLADQARLIRGACGISGPNLDYLINT
                     LAHLDSLGIHEPELRRLLAVIGAHFARGRFGNGGSTGAAALLKICRHLPVDVPHLKLG
                     ERRRFIHRRQISAWAARSRST"
     gene            complement(36808..37239)
                     /locus_tag="VF226_11340"
     CDS             complement(36808..37239)
                     /locus_tag="VF226_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074626.1"
                     /translation="MSARIVELSQIAAARAAAASPGHDLLNEPAAQQFQFWAGASGER
                     YVHSVFSLIGCPELPKAVYMLVRCDGNGRRIVLRVGRTEHDAPSLNLAEIRHRGAQLG
                     ANQVHVHFLAGSARERRRVELDLRTAHFSELSAERTSSMRH"
     gene            complement(37554..39962)
                     /locus_tag="VF226_11345"
     CDS             complement(37554..39962)
                     /locus_tag="VF226_11345"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX7074627.1"
                     /translation="MHPDPFSISPISEHIWQQKYRLKSADGEPVDRSLEDTFRRVART
                     AASVEKGGRKARERWTERYFEALSSFEFLPAGRIIAGAASGRQVTLFNCFVMGRIEDD
                     LSSIFENVKEAALTMQQGGGIGHDFSTLRPKGALVKSIGADASGPVSFMDVWDAMCRT
                     IMSAGARRGAMMATLRCDHPDIEEFVAAKADPARLRNFNLSVLVTDGFINAVREDRPW
                     DLVFDGRVYRTVRARDLWERIMRATYEYAEPGVIFIDRINDQNNLAYCEQIYATNPCG
                     EQPLPPYGACLLGSINLARLIDKPFEAGADVDLPRLEERVRIAVRLLDAIIDASNYPL
                     EAQRKEALAKRRIGLGVTGLADALIFAGARYGSPRALALAETWMAHVQRAAYLASTEL
                     AAEKGAFPLYDAEKFLARPGVARLAPEVRQAIATNGIRNGCLTSIAPTGTISLLAGNV
                     SSGVEPVFDFLYKRRVLERDGSHREMMVEDYAHAIFRSRFGDSVPLPDSFVTTAELPP
                     SAHLEIQAALQRHVDSSISKTINCPAGMSFEEFQNVYLDAYDLGLKGCTTFRPNPVTG
                     AVLSSRKMSEEPKGEAEERARGAKEPGKLEPSAVHRQLARSSEDGIVYMAKPLEREPA
                     LAGFTYKLKWPGSDHAIYITINDIEHDGRRRPFEIFINTRNLEHYAWTVALTRMISAV
                     FRRGGDVSFVVDELKAVFDPQGGQWMGGRYVPSLLAAIGGVIETHMVRTGFLQRREDI
                     APLIELMQAGAEIESAPSSSGTVGYAGSAGQRIRSCPRCNSLAFVREEGCWVCRDCGY
                     SRCG"
     gene            complement(40013..40792)
                     /locus_tag="VF226_11350"
     CDS             complement(40013..40792)
                     /locus_tag="VF226_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7074628.1"
                     /translation="MIRSLLFVAVFYANTALFLLIGSPLLFGPRSWAMWALKLHARVS
                     VWLLEAIVGTRMEVRGLERLPPGPILVAAKHQSAWDTFALIPLFRDPALVMKAELMLI
                     PLYGWFSRKFGMIPIRRSAGAAALRQMLHAARARAEAGREILVFPEGTRRPPGAPPDY
                     KPGLTLLYESLGIPCVPLALNSGLFWPRRSLKRYPGTIVVEILEPIPPGLKRAEFKAL
                     LLERLEGACARLIEEAGRAPHPPPVALEWLTQNRNINGTNC"
     gene            complement(40815..41459)
                     /locus_tag="VF226_11355"
     CDS             complement(40815..41459)
                     /locus_tag="VF226_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX7074629.1"
                     /translation="MTTMRRAWRLGFGFGLLVAALVVGFFLFARSAMHPAPSAVQKAD
                     GIVALTGTQSRISAAGQLLAKGLGKRLLVTGVNRQTTGAEIRQLAGLGEGLFDCCVDL
                     GYEAQDTIGNATETQAWAAKHGFSTLIVVTSSYHMPRSLVELGRVMPDVALIPYPVTG
                     PFQKESWWKSPGLIRLLLAEYLKFLPSAARYAVLRLVDGMEPRALATAEPSDRS"
     gene            complement(41605..42696)
                     /locus_tag="VF226_11360"
     CDS             complement(41605..42696)
                     /locus_tag="VF226_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074630.1"
                     /translation="MSGSYDRPTASSRFGSASGDFGAYEVADAPTGPPHVPQFEPVTQ
                     SPTVSTRREAYAPARAANRDPATTPIVPAGSVTGRSLTLVVSIMCFFACLTAGAVYMM
                     NQSAAAWLRDIASEVTVQLEPKDIPNMEGALNDVVAFLNRQPGVRGARALSVDESNAL
                     LEPWLGQLDILSTLPVPRLVALELDRTNPPDIPALRQTLATQFKGVTLDDHRHWQQQI
                     RTVTRSFALGGLAILLLVASATTAIIISATRSAMASNREIVEVLHLVGATDRFIAREF
                     ERHFLSLGIRAGFVGAICAMAVFFTMPAMMELLGGGTVTIAEMRRLVGTGALDTPGYV
                     LLGFVVVVIAGLCMLTSRFGVYRILHARQ"
     gene            complement(42680..43336)
                     /gene="ftsE"
                     /locus_tag="VF226_11365"
     CDS             complement(42680..43336)
                     /gene="ftsE"
                     /locus_tag="VF226_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX7074631.1"
                     /translation="MIRLENVGLKYGRGPEVLREVTFHLKPGSFHFLTGPSGAGKSSL
                     LRLLFMSLTPTRGQLHIFDQDVGRITPARRAQLRRRIGIVFQDFRLLDHLTTWENVAL
                     PLRVVGKRVSDYKEDVTDLLQWVGLGERMHAFPSVLSGGEKQRAAIARAVIGKPELLL
                     ADEPTGNVDPQMARRLLRLFIELNRLGTSVVIATHDHQLMRQYKAPRLELHEGHVRIV
                     "
     gene            43630..44178
                     /gene="hpt"
                     /locus_tag="VF226_11370"
     CDS             43630..44178
                     /gene="hpt"
                     /locus_tag="VF226_11370"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX7074632.1"
                     /translation="MSAGNGENDRIKVVFSPEEIARRIDALAKRIAEQNLERLLVVAI
                     LKGSFVFAADLIRALHRVGLEPEVDFMSLSSYRKSTRSSGKVEILRDVEMDVTGRNVL
                     IVDDILESGRTLAFAKDLITARGARKVLTCVLLDKPVRRAVQVSADLAAFECPNVFVV
                     GYGMDLAHRYRELPFVGQLSTV"
     gene            44216..44569
                     /locus_tag="VF226_11375"
     CDS             44216..44569
                     /locus_tag="VF226_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7074633.1"
                     /translation="MARILLVDDDPATLDFIGRALSADGHEVLSCADAQEALQKLSAG
                     PVDLMLTDLGMPGLDGLGLARRAAKALPSIRILLMSGFASPDLPAAQALPIAGFISKP
                     LSLEQVRSAVRSALV"
     gene            complement(44566..45531)
                     /locus_tag="VF226_11380"
     CDS             complement(44566..45531)
                     /locus_tag="VF226_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7074634.1"
                     /translation="MPAPKTTERDRGGHLHTAMLSSLRSVAAGSDNLRGILSMIAAMT
                     SFVAGDSIIKFAGQELPPGEMMFVRGLFTSALTLGVAYITGALRRAYLVATPPMLLRA
                     VGDVGATLFFFMALLRLPFAEVSAISQFAPLAITAGAALFLAEPVGWRRWMALVAGLT
                     GVLIIIRPGGAAFDWAALFVMGSVLCVAMRDLLTRHIGLSLPALLLTSYSAVSVTLGG
                     LVLMPFESWRMPTADLLILLIIAGVTVSAGTFFTVMAMRAGDMAVVSPFRYVATPLAV
                     ISGYFVFAELPDNITFIGIAIVMGAGLYTLHRERLSLRSRFARIA"
     gene            45862..46635
                     /locus_tag="VF226_11385"
     CDS             45862..46635
                     /locus_tag="VF226_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074635.1"
                     /translation="MILECRDLERSFGGLKALQGVTLSIDKGEIFGLVGPNGSGKTTI
                     VNSITGFYPPDRGNILFEGKDITGIRPHNVARLGIARTFQNLALFRGMSVLDNVMMGR
                     HIHMRPSALASLFYWVWAERDEIRQRELVEDTIDFLQLQDVRDLPIEVVPLGLQKRIE
                     LARALVSEPRLLILDEPMAGMNQEEKEYMARFILDAREERGLTVLLIEHHMDVVSALC
                     DRMTVLSYGEQIASGTPAEALSNPAVVEAYLGTKRRHAA"
     gene            46625..>47415
                     /locus_tag="VF226_11390"
     CDS             46625..>47415
                     /locus_tag="VF226_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7074636.1"
                     /translation="MRPEGSTAKAVELGLDLASRDPDAVPRENAGLDSGTLISLLAKN
                     AETHGSSIAVRERDRGIWQEFTWSDVLSEVLAFAAALEARGFRAGDGILVIGDNRPRL
                     YFGMLSAAALRGYPAPVYPDVPPDDLEYYAGSSRARFALAEDQEQVDKLLELRNRAGL
                     PEFIVYDDPRGLWNYTEPGLLSWEDLVREGAERLAREPGLKEDLIKRAKPTDAAVLLH
                     SSGTTGKPKGVVLQHRRVIAAARNAEKADYFRAGDELMAYLPMAWV"
BASE COUNT         8475 a        15355 c        15282 g         8303 t
ORIGIN      
        1 agaagagttg cacgtcggcc tcaaggaagc gattgacgcg gccggtgtcg atctggtctt
       61 tgcatgcggc ccgaacatgg gccggctgtt cgccactctg gagccgggtc ggcaagcggc
      121 ctggaccgag acgtccgaag ggcttgccac acctctgctg ggggcggtcc gggcaggtga
      181 cgcggtcatg atcaagggat ctttgggcag ccgcatggct cccctcgccg aggctctgaa
      241 aaaccggttc gcaacttgag attgccggcc aggactgggg aagccaaacc ctgctgaatg
      301 ccgccggtcg gagtgcgtga aggataaatg catgctctac gagctcgtta atttcagcga
      361 ctacatcaac gcactcaacg tcttccgtta catcaccttc aggaccggcg gcgccatcat
      421 gacggcgctg ctcttcgtct ttctcttcgg ccccatcatc atcgacctgc tgcggctgaa
      481 gcaggggcgc ggccagccga tccgtgatga cggcccgcag tcgcatctcg ccaagcgcgg
      541 caccccgacg atgggcggcc tcatgatcct ctcgggcttc accgtcgcga ccctgctgtg
      601 ggccaacctg tccaatcctt acgtctgggt cgtgctcggg gtgacgctcg cttttggcgc
      661 gatcggcttc tgcgacgact acatgaaggt gacgaaaagc accgttgccg gtttttcggg
      721 ccgcctgcgc ttcgcgatcg agcttggcat tgcgggcata gctacgggcg cgctcatgtg
      781 gctgtccgtc ccggagctga cgtcggctct cactgtcccc ttcttcaaga acatcgtctt
      841 cgacctcggc ccgttcttca ttcttctcgg cgtgatcgtg atcgccggcg ccggcaacgc
      901 cgtgaacctg acggacggcc tcgacggcct cgccatcgtc cccgtgatga tcgccgccgc
      961 cacgttcggg ctgatcgcct acctctccgg caacatcaat tttgcgcgct atctccagat
     1021 ccactacgtg ccggggacag gcgagctcgc catcctctgc ggcgcactga tcggggcagg
     1081 actcgggttc ctctggttca acgcgccgcc ggcgatgatc ttcatgggag ataccggctc
     1141 gctggcgctc ggcggcgcgc tcggcaccat cgcggtggcc acaaagcacg agttcgtgct
     1201 cgccatcgtc ggcggcctgt tcgtgatcga gacgctgtcc gtcatcatcc aggtggtctc
     1261 gttcaagctg acgggcaaac gtgttttccg gatggccccg ctccaccatc attttgaaca
     1321 aaagggctgg aaggaaagca ccgtggtggt ccgcttctgg atcatctccg tggtgctggc
     1381 cctgatcggc ctcgccacgc tcaagttgcg ctgaggcggc tccaacgcac tacggcgcga
     1441 aatccggtag aatcgcgggc atgattcgcg tcaccacatt cgccggcaaa acggttgccc
     1501 tggtcggcct cggcgcatcg ggccgggcga ctgcgaacgc gctgctggcg ggcggcgccg
     1561 aggtcgcggc gtgggacgac aatgcggagg cacgcaccgc ggccagccaa gcaggcattc
     1621 cggttgcgga cctctccgca atggattgga ggggcttcgc agcccttgtc ctcgcccccg
     1681 gcatcccgtt gacgcacccg gagccccact gggctgttgc aagggcccgt gcggctggcg
     1741 tcgagatcat cggcgacatc gagctgttct gtcgtgagcg cgcctcgcgc tctccggacg
     1801 cgccgttcgt ggccatcacc ggcacgaacg gaaagtcgac cacgacggcg ctgctggccc
     1861 acctcctgcg tgcagccggt cgcgacgtcg ccatgggcgg caacatcggc acgccgatcc
     1921 tagcgttgcc cgagcctgcc tccggccgcg tgcacgtcat cgagatgtcg tcctttcaga
     1981 tcgacctgac gccatcgctg gccccgacgg tcggcgtcct gctcaatttg acgcccgatc
     2041 acctcgaccg gcacggcacg atggagcatt acgcggccat caaggaacgg ctggtgacgg
     2101 cgtcgtgcca tccgatcatc ggcgtggacg acgactattg ccagcgcatc gccgatcggt
     2161 tgcgcaccgc ccggaacggc gatttggacg cgctctctgt ccttcggccc ctccccggcg
     2221 ggtggttcgc ggacggctca cgcctccact cagatgcgga ctgggccggc atcagcgcgg
     2281 tcttcgccga tctcaatggc atcgggacgc ttcgcggccg ccacaacgcc cagaacgcgc
     2341 tggcggcctg catcgcggct ctgcgcctcg gcgtcctgcc cgaccagctc cagccggcgc
     2401 ttgccacctt tcccggcctg ccgcaccgga tggaggaggt cgggcgcgcc ggccgtgtcc
     2461 tcttcatcaa cgactccaag gcgaccaatg ccgactcgac cgagaaggcg ctggctgcct
     2521 tcccgcgaga catctactgg atcgtgggcg gccggcccaa ggagggcggg atcgagccgc
     2581 taaggccgta cttcccccgg atcgccaagg cttacctgat cggtgagtcg accgaatcat
     2641 ttgccgcgac gctcggcgct ggcgtcccct tcgagcgttc cggaaccctc gacattgccg
     2701 tccggactgc ggcgcgcgaa gcggcgcaga gcgcgggtgc cgagcctgtg gtgctgctgt
     2761 cgcccgcctg cgcctcgtac gaccagttca agaattttga acagcgcggc aacgtctttc
     2821 gcgaattggt ggccgcgttg cccggcgtaa ccgtgccggg gcggccatga agatttcgcg
     2881 gacagaccgc agccttgttg ccgaatggtg gttcacggtc gaccgtgtgc tgctcacggc
     2941 catcctgatt ctggccggga gcggcctcgt gctgtcgctc gcggcgagcc ccgccgtggc
     3001 cctcaagaag gggttttccg cattccactt cgtcgagcgc catgcagcgt tcgctggcct
     3061 cggcgtgctc atcatgctgg ccgtctcgat gacgcagcca cgactcatcc ggagactggc
     3121 gctgatcctg ttcgtcggcg gaatcgtgct gatggtcggc gttctggcgt tcggcgaaga
     3181 gatcaacggg gcgcgccgct ggctgcgcct tgcaggcttt tcgctccagc cttcggaatt
     3241 cgcgaagccc gcgttcatcg ttctgactgc ctgggcattc ggggaggcgc agaagcaggt
     3301 cgatatgccg gcacggagca tcgccattgc gcttctcgcc gtattcgacc tccttctgct
     3361 ccgccaaccg gacctcggcc agacgttcct cgtcagcgcg atctgggcga cgctgttcgt
     3421 gctgtccggc cagccgatca tctgggcagg tatcttcggt gtcgccggag tcgtcggact
     3481 gacaggcgct tacttcacct tcgattacgt gcgcctgcgc gtcgaccgct tcctgagccc
     3541 cggtggtgac gaatactcgc agaccgctcg cgcgctgcag tcgttcgtcg aaggcggctt
     3601 cttcgggcgc ggccccggtg aggggtcgat caagaccgca cttccagacg cacacacgga
     3661 tttcatcttt gccgtcatcg ccgaggagta cggcgccatc gcctgcctcg ctctggtcgc
     3721 cttgattgcg atcatcgtct tgcgcgccta cgtccgcaca ttcacggaaa ccgacccttt
     3781 cacaaggctc gccgtggcgg ggctctcgtt gctgttcgga cttcaggccg cgatcaatat
     3841 ggccgtcaac gttggcctgc tgccggccaa gggcatgacg ctgccgttca tatcggccgg
     3901 cgggtcctcc ctggtctcga tgttcctgac catgggcatg atcctcggac tgacccggcg
     3961 gcgccccgac gtgttgcgct tgagaaggcc gcattcggcg ctcaagaatg aggacagcct
     4021 cggcagattg ggatccgcag cgcagtgaac gccccatccg tcatactcgc ggccggtgga
     4081 accggcggtc atctcttccc ggcgctggcg ctggcgcagg agctcggccg ccgcgccatc
     4141 gtcgtcgatc tcatgacgga cacgcgcgga gaccgctacg gcagtgattt tcccgcgcgc
     4201 cacatctacc gtgtgccatc ggcgacgatc ggcaagggac acggctttct cgacaaggca
     4261 aaaacggcgt tgacgctcgg gcgcgggatt ctcagcgcct acaggcagct tggctcgatc
     4321 aagcccggcg ccgtgatcgg cttcgggggt tatccgagct tcccgccgct ggtcgcggcc
     4381 tttctcaggc acattccgac ggccattcac gagcagaact ccgtgctcgg gcgagccaac
     4441 cgcatgctgg ccagccgcgt cacggccatc gccacctcgt tttccgcgct gcaccacctc
     4501 gacgccgccc tggaagccaa ggtgcggctc actggaaatc ccgttcgcga tgcggtgatc
     4561 gaccggtcct cgcgggccta tgcgccgccg gcatccgatg gcaccttcac gctactcgtt
     4621 ttcggcggca gccagggggc gaggttcttc tcggacaccg tgccggcggc catcgccctg
     4681 ctaaagcccg aaacacgcca gcgcctcaga atcgtccagc aatgccgcga agaagatctc
     4741 ggccgcgtac gtcaagccta cgcggctctc gaggtcgaca tggaacttgc gagcttcttt
     4801 cgcgatctcc cggagaggat ggccgcagcg catctggtca tcgggcgcgc cggggcatcc
     4861 tccgtagcgg aactgaccgt catgggtcgg ccctcgatcc tggtgccgct gccacatgcg
     4921 atcgacaatg accaactgcg gaacgcaacg cagctcgcaa agagcggggg agccatttgt
     4981 atcgagcaga aggacctgac gccggagctg ctggcccgcg aggtcgagcg gctgtctgcc
     5041 aatccggagg cactggcggc gatggcggcg gcggccaaag cgcagggccg gccggacgcg
     5101 gtcgtccgtc ttgccgatct catcgaggag ctcatggggc tgcggcagcc gccgcgccag
     5161 cccgatgagc ggggcaagca ctgaggatta gcccatggaa ataccccgcg gtatcgggac
     5221 cattcacatt ctcggcattg gcggcatcgg catgagcgcg atcgccgaaa ttctgcacgc
     5281 caaaggcttc agcgtgcagg gcagcgatca gaaggagagc gccaacgtca agcggctgcg
     5341 gggccagggc atccgcgttt tcgtcggcca tcaggcagag aacctggccg acgcgaaata
     5401 cgtcgtcatc tcgagcgcgg tgaaggacgg gaacccggag ctcgaagccg cgcgcgccag
     5461 gggtttgccg ataatccgtc gcgcggagat gctggccgag ctgatgcggc tctacgcaac
     5521 cgtttcggtg accggcacac acggaaagac gacgaccacc tcgctcgtcg cacacatctt
     5581 caaggaggcg gggctggacc cgaccgtcat cacgggcggc atcatcaacg actggggctc
     5641 gaacgcaagg cttggagcga gcccttggat gatcgtcgag gccgatgaat cggacggtac
     5701 cttcgtcaag attccgaccc agatcggcgt ggtcacgaat atcgacccgg agcatctcga
     5761 ctatttcaag acggtcgaga acatgcaccg cgagttcgag gcgttctatc gcaacatccc
     5821 attttacggg cttatggtga cctgcacgga ccatcccgtc gtgcgggaca tggtggaccg
     5881 gctccatctg cgccaggatg ggcgccggct ggtcacctac ggcacgcaag ccgatgcgga
     5941 catggtgctg agcgaggtgc ggttcgacgg cctcgacacc attttcgatg ccgagctgcg
     6001 ggaacgggtg agcggcggtg cccgcagcat tcgcggatgg cgcgttccag tgccgggcca
     6061 gcacaacgcg ctcaatgccc tggcggcgat cgcggtggcc gccgaagccg gcattcccga
     6121 cgacgtcatc cggcaggcgc tcgcatcgtt cagcggcgtg aagcggcggt tccagctcac
     6181 tggcacctgg aacggcgtct tcatcttcga cgactatggc catcacccgg ccgagatctc
     6241 agccgtcctc aaggcggccc gcgcgggcgc ccgcgggcgc gtgatcgccg tcatcgagcc
     6301 gcaccgctac acgcgggtgc gcgacctgtt ccaggacttc tgcgcgtctc tccaggcggc
     6361 cgacagcgtg atcgtgacac cgctctactc ggcaggagag gcaccgatcg acggaatcga
     6421 tcacgtcgcg ctcgccgagg gcatccgctg cacggggcac gcgtccgtgt ttcccgtcga
     6481 cgacgagcag gacgtcgtgc cggttctgcg caggatcgct cagccgggcg acatggtgat
     6541 ctgcctcggg gctggaaact ccaccgagct tgcccatgca cttcccgagc tgctcggcgg
     6601 tgaggccagg ctcgcggggg cagcatcatg agctttcccg atttgacagg ggacctcacc
     6661 gcgcgcatgc cggatctcag gggccggctg gttgccaact tcgatctgtc gagcatcacg
     6721 tggttccgcg tgggcgggcc ggcgcagctt cttttcacgc ccgccgatga agccgatctc
     6781 gcctatttcc tatcccgcgc tcctgccgat ctgcccgtga ctgtcatcgg cctcggctcc
     6841 aatctgctgg tccgggatgg cggcgtgccg ggcgttgtca ttcgcctcgg gcgcgggttc
     6901 tcggaggtca aggtcgagcc gggtcatcgc ctccgcgccg gcaccgccgt gccggatgtg
     6961 aaggtggccc gcgcggcggc tgacgccggc attgcgggcc tcgcattcta tcgcggcatc
     7021 ccgggctcca tcggcggcgc gttgcggatg aacggcggcg cccacggcac ggagacgaag
     7081 gacgttctcg tcgaggcccg cgccgtcgac cgcaagggca acatccacgt cctctcgctc
     7141 gctgacatgg gttacagcta ccgccattgc agcgtgcctg gggactggat ttttacggaa
     7201 gccacctatc agggccggcc gggcaaggcg gaggacatcc tcaagcagat ggaagaggtt
     7261 gcccaatacc gcgaggagaa ccagccgacg cgcgagcgaa ctggcggatc caccttcaag
     7321 aatccgccgg gtcacagcgc ctggaagctc atcgacgcgg ccggctgccg cggcctgcgc
     7381 atcggcggcg cgaaggtttc cgagatgcac tgcaatttcc tcatcaacga ggggcaggcg
     7441 accgcggaag acatcgagcg gctgggcgag accgtacggg caagagtcaa ggcgaattcg
     7501 ggcgtgaccc ttcagtggga gatcgtccgg ctgggtctgc cactcgacgg ccgtccgact
     7561 ggggaggctt tggcagcgtg aattgcgctc ttcgaaaaaa aacgtcatcc cggtgcttgt
     7621 caccgggatc caatttcctg cttgctccgg cattcgcggt gagctggatc ccgggcacaa
     7681 ggcccgggat gacacgtttc gtgtgcaggc gagcaggggc atttgagtca tgagtgcaga
     7741 tagtgcgggc cgcaaatacg agcacgtcgc cgttctgatg ggcggatggt cggccgagcg
     7801 cgaggtgagc ttgagctcgg gcaacgcctg cgcagcagcg ctcgaggcag ccggatatcg
     7861 cgtcacgaaa atcgatgtcg atcgcgatat cgcccgcacc ttgaaggaac tggcaccaga
     7921 cgtctgcttc aacgccctcc acggacgctg gggcgaggac ggctgcattc aggggttgct
     7981 cgaagtcttg ggtattccct acacccactc cggcgtgcgc gcttccgcgc tcgccatcaa
     8041 caaggagcag acgaagcggg tcgtctccgg cctgggcgtg ccgacgtcgg agcatcggat
     8101 cgtcagccgg cgtgaggcgg tacgcacgca cgcgatggag ccgccttacg tggccaagcc
     8161 catctgcgag ggctccagcg tcggcgtggt gatcgtgcgc gaaggcgcca accggccgcc
     8221 cgaggccatc gccaccgttc cggcgatcga agacgagatc atgatcgagc gattcatcgc
     8281 cgggcgcgag ctgacctgtg cggtcatcgg cggtcaggcg accgatatca tcgagatcgt
     8341 gccggcggcg cggctgacgt tctacgatta cgaggccaaa tacgctccgg gcggctcgcg
     8401 gcacatcctg cccgccgaca ttccgcgcga cgtttatgaa gccgttcagc gccactccgt
     8461 gacagtgcac aatgcgctcg gctgccgggg cgtttcgcgc tccgatttcc gctacgatcc
     8521 ggcaacgggc cagctcgcgt ttctcgaaat caacacccag cccggcatga ccggcacctc
     8581 actggtgccc gagatggccg ctcacgccgg catgagcttt cagcagctcg tctcctggct
     8641 ggtcgaagat gcttcctgca accgttgata gctcaacggg atagcggaca ccgctcaact
     8701 gaaaattaac ctgttcgcac gaacttggcg tgtggaatgc cggtttgggg ggactggctc
     8761 cattcacgca ttcgttgcga acggagagtc cgcgtgcacc gaaccgcacc agctgcgatg
     8821 ccttcgcggg cctcgagccg cccctcgggg ccgatgaagc tttctgccgc tccgatgccg
     8881 aaacggccgc ggccgcggcg gcagagcgcg gttcgcctga cggcgtggac gaccgcaatc
     8941 gcgggcgctg ctctgttcat cgcgctgacc cagtggggag ccaacgcacg gcaagcgcgc
     9001 ccggtggtgg aagaggcgga ccggctcgcc gagctcgctg gcctgggcgt gcagcaggtc
     9061 tatctcaccg gccaccggat gacttcggac agagacatct tcgacgcgct cgacctgacg
     9121 caggcccggt cgctgctgcg cttcgacagc gtcgccgcgc gcgaacgcat cgagcgactg
     9181 ccgtggatca aaactgcggc catcacgcgg atctttccgg acagcatcag catcgaggta
     9241 gtggagcgga aggcattcgc cgtctgggaa gagtccggga aggatctgct gatcgacgcg
     9301 acgggccgcg tcctcggcct cgccccgccc agcatgcaag ccggcctgcc gcgcttcgtg
     9361 ggcagaggtg ccaacgagaa agcggccgca ctcctgtcgc tgctcgagca ccagccctac
     9421 gtccgcgccc gcctgaaaga agccgtccgc gtcgccggcc gccgctggac gctgcggctt
     9481 gccggcgggc tcgagatcca cttgccggcc gggcgcgagg cggaggcgct ggcacggctc
     9541 tcgtcgagcg tggcggccag ccgtctgctc gacgcgaacg agtacgctgc gatcgacctc
     9601 cgcccggccg accgcatcat catccgacca gtacacgacg gggagaggaa aaccgcatcg
     9661 agccagcacg tccaaccctc gggatgacgt gaactcagga gatgatctga tgccgttcga
     9721 ctcatcatct ggcgacgtcc tcggcctgat cgacatcggc acgaacaaaa tctgctgcct
     9781 gatcatggtt cgcgatcgcg gctacaggtt cggccgaggg gcgaggagct atcgcgtggc
     9841 aggcatcggc catcagcgct cccgcggcgt gaaggctggt gtgatcgtcg atctcgacga
     9901 ggccgagcag gccgtgcggg ccgcggtcgc gcaagccgag cggatggcag gcttgcgcat
     9961 cgaggaagtg gtcgtcggcg ctgcctgcgg gcgcatgcgg tccagccatt tcgccgctca
    10021 tgccgacatc gagagcggct acatccgcga gtccgatctg cgccgcctga aggcggccgg
    10081 gcgcgcttac gctgaaaggg atggccggct actgctctat ctcgaccagc tcaactggcg
    10141 catcgacgga gctcccggta tccgtgatcc gattggcatg gctggccggc ggctctccgc
    10201 agacctgcat gcgatcacgg cggacgatgc accgctccgc aacctgatgc ttctgatcga
    10261 gcgctgttat ctgacgctca gccgggccat cccctcgccg ctcgccagcg cgcttgcggt
    10321 caccagtgag gaggagcgcc gcctcgggat cacctgcctc gacatcggcg gcgggatgac
    10381 gtcgatctcg gtgttcgtgg acggacggtt cctgttcgcc gattccgtcc cggtcggcgg
    10441 caatcacatg acctacgata ttgcgcgtca gctcaggacc cctcttgcgg aagccgagcg
    10501 aatcaaggcg ctttatggca cactggtcgc agctaggtcg gatgagcacg aggtcatcac
    10561 gtatcctctg gcgggagaag aggaacagac gctgcatcac acgacgaaag cgcggctgcg
    10621 cagcatcctg cagccacgaa tggaagggtt gctggagctg gtgcgcgaac ggctgcagcg
    10681 cagcaacatc aagagctacg cgggggagcg cgtggtgctg acagggggga cgagccagct
    10741 gatcgggatc ggagaattcg ctgcaaacac attgggccgg cccgtgcgcg tggcgcggcc
    10801 gcagcctatc ggcggcatgt cggcgagctc atgcagccct gcgtttgcga ccgtcatcgg
    10861 cttggttgcg gctgcggagg tttcacagtc ctgctgggca tctttcccgg aatatccgag
    10921 ctcgcaggaa agttacttcg gccgcatggg acagtggctt cggcagagct tctgacgcgg
    10981 tgaggagcga gagagcggtg atgaattaca acctgcacaa atatgcggag cacagcccct
    11041 ctcgcccgct tcgaggctgc atgggagcat catgagcatc agtctgcagc ttccgaaagt
    11101 gacagatctc aagccgcgca tcgcggttat cggcgtcggc ggcgcgggcg gcaacgccgt
    11161 caacaatatg attgcctccg gcctcagcgg cgtgagcttc atcgtcgcca acaccgatgc
    11221 gcaggcactg gccgcgtcga gcgcggagca tcgcatacag ctcggcacca atctcacgga
    11281 aggattgggg gccggctcga agcccgagat cggtgaagct gctgcggaag aagctgtcga
    11341 tgagatccgt gcccagatct ccggatctca catggttttc atcgcggccg gaatgggcgg
    11401 cggcaccggg accggagccg caaccgtcat tgcccggatc gccaaagagc tcgacatcct
    11461 cacagtcggc gtggtgacga agcccttcca tttcgagggc tcgcggcgca tgcgtattgc
    11521 ggaagccggc atcgccgagc tgcgcaagat cgtcgatacg ctgatcgtca tccctaatca
    11581 gaacctgttc cgcgttgcca atgagaagac gacgttcgcc gaagcattcg tgctggcgga
    11641 ccaggtgctc tattcaggca ttgcctgcat cgtcgatctg attgtcaagg aaggcctcat
    11701 caacctcgat ttcgccgacg tgcgcacgat catgagcggc atgggcacgg ccatgatggg
    11761 cacgggcgag gcaaccggcg atcagcgcgc catcatggcg gcggaggagg cgatttccaa
    11821 tccgctgctc gacgacatct cgttgcgtgg agcgaagggc ctgctgctgt cgatcatcgg
    11881 cggccgcgac ctgacgctgt tcgaggtcga cgaggcagcg agccgcgtgc ggcaggaagt
    11941 tgacccggaa gcgaatatca ttgtcggagc taccttcgac gagagcatgg gcgaccgcgt
    12001 gcgcgtgtcc atcgtcgcat ctggcatgcc gccggaggag accaagcccg ccgcacccgc
    12061 aaccggggca taccggtcgt ccggccggca ggcacctccg ccgcagcacg cggctcctca
    12121 cccggcaccg caggccccta tggatcggcc ggccgcggcg gcgccggggg tatcatccgg
    12181 gccgtccccc aagggatcgg acaacttcat ggatgccctt tccagggcaa tcgaagaaaa
    12241 acctggccaa acaagcggcg aaccgaagcc caaggacgtc tggcatggcc ctggagatgt
    12301 cgtgatcgag gagggcctgc cgccgatcgg ccttggccca tcaacagcgc caccaccacc
    12361 ctcccggggc gctggagcac aggcgggtgc acgcttgccg gaacccttta ctcccggccc
    12421 tccggtcgag gtgaagcgcc agcaccggcg catgccgacg gttgaggatt tcccgcccgt
    12481 cgcgcagcgg gagtaccgcg cgaaggctgc ctggtatgaa acggggccct ccactccgcc
    12541 ttcgtcgcgc ggctacgctc cgcaaaagga accggccaag aaggccagct ttttcagccg
    12601 aatcaccggg cttgggtggc gcacggagca accggcggaa cggcatgctc gcgagaattc
    12661 acacaatcct ggccgtgcac acgatggcat gtcctcggac catgcatcaa ctccaggtcg
    12721 cgcacctcac gaaggcgatc gaccggatga tcgcctggga tcgcaaggtg ctgattttcc
    12781 catgtttttc cagaaggatc gtaagcgtca gtagtcttac tgacgggtta ccgcatggca
    12841 cggcctaagc ctgcagcttt gtggtaacag tatgtaagaa agcgtgattt gtccccagac
    12901 gggacaccac gttatcgtcg gtgcatcaaa tcgttcgccc actgcgggtc ccaggtctca
    12961 agtaaagaaa tgaccgcgta gcaccggcag gccagtgaac gaacgggggt aggcaaattt
    13021 gaacggctcc ggccggatcc gtttgcctag ggatcgaggg acggcgcaga catgtcgaag
    13081 cgattcatcg gcgcgcgaca gacgacgatt gcccgagagg tcgagcttca aggaaagggc
    13141 gtccacagcg gggcgccggt ctccgttaca ctgcatccag cagggccgaa tacaggcttg
    13201 aagttcttag tgaataaacg tggccgaatt attgcggaaa tacctggaga agtcggctac
    13261 gttaagaatc tcacgctctg caccgtcata ggcgacgact cgggcgtcac tgtcgggacg
    13321 gtcgagcatt tgctcgcagc gctccgtgga ctgtcggtcg acaactgcta catcgagatc
    13381 gacagcaagg aagtgccgat catggacggc agcgcggcgc cgttcgtgga agcgatcgat
    13441 caagccggca tccgtgagct gccggagccg cgcaagtaca tcaaagtgct caagccgatt
    13501 cgtgtcgagg aaggcgcgtg ctggggcgag ctgacgccgc attccgggtt tcatctcgac
    13561 atcgagatca acttcgatac gccgctcatc gggcagcagc gcttggcctt cgatatcaat
    13621 ccgggtgtgt ttcgccacga gctgtccatc gcccgcacct tcggcttcat gcacgatgtc
    13681 gagaagctct ggaaggctgg gttggccctc ggcgcgtcgt tggataacac gatcgccatc
    13741 ggtgaggacc gcatcctcaa tcgtgagggc ctccgttgtc cgatggaatt cgtccgtcac
    13801 aagatgctgg atgccgtcgg tgatctcgcc ttgtcgggct atcctctgct cggagcctat
    13861 cgctccgtgc gcggtgggca tcgtctcaac gcaagtgtgc tgaaagcgct gtttgccgac
    13921 gcttcagcgt ggaccctggt tcaggccccg cgcgtacgcg agccgttcgt tcggcttccg
    13981 gcggcgatgg cggtggcggc gggcgagtga agagcgccga tacccaacaa tctagcacca
    14041 cctcgcggcc ttctccttcg cgccggctac tgcttgctgt tcggcagaat actgcagcgg
    14101 ttggtggtct gcccaatcgc gaccacaaat ggccgctcta aagaaggggg ataacggctt
    14161 tcccttcggc agcttgccgc cggatgtgtt tcgaatcgat tagaagcagg ctgcaagggc
    14221 tatctgtggt gcgactagtc cgtcgtcgaa acgcgcgacc gtgatgcccg acaacagaac
    14281 ggtggagtgg gggcgaaagt gttggcagac cacatgcacg gaaagcggcg agctggcctg
    14341 gtttccgccc tgctggcgct ttgcgtcgtg atgagcggtt gcgcttcctc ggacgacgcc
    14401 gcaaagttgc tcaaccctga tccgcccgaa aaaatgtatg ccgaagccga tcagctgatg
    14461 agccgcggca aatacgaggc tgcagcccag aagttcgagg atcttgatcg cgaccacccc
    14521 tattcccagg aagcgcggcg tgcaatggtg atggccgcgt acgcctatta tcgggctggc
    14581 aagacgcagg aggcgatcgc cactgcgcgc cgctacacca cgatgcaccc gggcaccaag
    14641 gaagcgccgc ttgcgcacca catcatcata tcggcgtact tcgacgagat ggtgggcccg
    14701 aaccgtgacc agacgaatgc ccggaaggct ctggccgagc tgaaaactct gaaggcgcgc
    14761 tatccagata gcccctatgc gcgcgatgcc gacaaccgga tccgcatcgc cgaggactcc
    14821 attgcagcgc acgagatgga ggtcggccgg tactatctga aacgcaaaaa ctatatggcc
    14881 gccatcaacc gcttcaagac ggtcgtcacc gagtaccaga ccaccgcaca cgtggaggaa
    14941 gcgctcgagc ggctggtgga agcgtacatg gcgctcggca tcaagcatga ggcgcagacg
    15001 gccgccgcgg ttcttggcca caatttcccg tcgtcgccct ggtacaagga cgcctacgcc
    15061 ctcctccagt cggacggcct tgccccgcgc gaggacaccg gctcctggct cagccgtgcc
    15121 tggcagaact tcaagctgcc gaagctcacg agcgggtgag ggttccggtc gcgctcgatc
    15181 atgatgaggc cgacgctggc ttcccgcgcg gagacgagcc taatacatag gtttctgccg
    15241 agctggacaa tgaacacttg gcgtgcaccc atggcgactg gaatatttct gatgaaaatg
    15301 gaagcacgct aggtcaaatt ggtgccagcc tccctctccc cggttagggt gaggggattc
    15361 cgccagtggc gaccgttgca acccctcacc cggcccttcg ggacgacctc tccccatggg
    15421 agaggtgaag tgggcaatcc ctgctggctc cgttacggcc taattcgaaa attctatagt
    15481 cggcgctagc ggatcagcgg acgtcatcgt cgacggagaa gccatgggcg gagagtgccg
    15541 tgcggcagcc ctgcgagacc tgttccgcat tgtccagaag gcacaccaaa gcccggccca
    15601 ttccgggccg gacgccggga cacagccgct cgaaatcggg ccggcacagg cgcagcacgg
    15661 cggcgaaggc caagccccgc tctgccaggg cctgaaagca gggctgtgac agcttctccg
    15721 cctgcgcatt gagacacacg agcacgcgtc cggcgcccgg gcgtacgccc ggacagagcc
    15781 ggaagtaatc cgccctgcag gcagcgaccg cctcccgcat ctgaatatag cggaggccct
    15841 gtgccggagc tggttccgcg aaggtcaggg ccgcggccaa acacaggaaa gcgattgtcg
    15901 cgcgagagcg cattcgggcg tcgggcctcc tccagcctgc ggtacggacc atgtcgccgc
    15961 cgagttcgcg gaccggattt cgtttcgttg ccgagcacca cccgttagcg gatcaccggc
    16021 cgcaccacaa cgatgctact tgagcggcgc atcgtcgaga tccggcgggt tgacggtgaa
    16081 atcgtccgct gagccaaagt ctcgtggaaa accgggacgc cggtgatgct ccacgtaagc
    16141 ctgctcgaaa tggtagggaa aggtcgcact taaagcgcga acgcattcgc gggataggcg
    16201 gtcgaagtta ccttcgagac aggccgcacc acgtcccgcg ccgggcacga cgccagggca
    16261 gtactcgtga aaatcaccgg cgcacgcgtc tatcgtcgca acaatcttca gcgcctgccc
    16321 gcacggcctc gaaagggcgt gatgctgttc ggccagacag cgcagggcct cgccggtacc
    16381 ggtcggcacg ccccggcagt accgcacgta gtcggcataa cagacccagg cgagcacccg
    16441 cgcccccggc ggacccgcga cccaggccgc ggctggcgca gcgcgcgtgg cgagaaccga
    16501 tagggcaacg aaggcggctg cagtcagtcg ggacagcata ggcatgtaat cctccgctcg
    16561 actcaggatt ttgtggcaag caatggccgc gaccaggcac gctgggggac gggcgcccca
    16621 gtcgcggcgc cggttaccag cggcaatggc ggatgccgag ccagtcgacc cagcagcggt
    16681 gcggccgcgg ggccagcggc acacacgcga tcgggcgaca gaagcgcccg tggcggtgcc
    16741 agcggcgcac cggtccccac tcgcacgacc tgtggcagcc atggactttt tggactgcgc
    16801 tctcggagcc tgccggctga acaggactcg cgggcagtgc ctgggccgca cttgccagca
    16861 ccgaagacac ggccacagcc gcaaacgtca agattgctcg gttcgtcatc gtgctcatcc
    16921 ttttttgggg ttccccgaca gcgggggcgt cacaggggca attgagcaca gccggcgcga
    16981 tccaatcgtg tgtgggcctg cctgcgtacg ccaccgctgt cggggttcga atcagtcgtg
    17041 cttgttcttg tagatgcgca cgttctgcac gttcagacgg tgatgcagaa tcgcgcgttc
    17101 ggcgggggta acgacaccgt cggacttgaa ctgccgctcg aggcggcgga cgcgatgctg
    17161 tcctctgatc agtctcgcgg cttcgccgta tgtcagctcg ccgcttttga tgccgttgta
    17221 gatccggtgc gcttgccgat gctcgcgctt atcgacataa ggcgttccgg caagagctgg
    17281 cccggaagca accaccagag caagtccggc caacagatac gctttcatga tctctctcct
    17341 ctgagattga gatttgacca gtcgcggcgc ccggagctct tcgtcaccga gactgccgcg
    17401 gcccaggggt gtccccaagg cccggcaaat gtgccaagct cagatcacgt gcttttgccg
    17461 gggcggtaac tcctttcaac gattgttgag ggcttgtaac gacgtgtaac aacggcgatg
    17521 gcggacttgt ccgcgacgct ttccgttttt aacgtccgcc catgatccag gaaccgcagg
    17581 cgctcatcgc gatcgttgag gacgacgagg agatccttcg cctcgtctca agtctgcttt
    17641 cgcaggaagg ctatgcggtg gaggcggccg agaacggtgc ggcgctcgat gcggtccttt
    17701 cccggaccag gccggatctc atcattctcg atctcatgct gccaggcgaa gacgggctgt
    17761 cgatctgccg ccggttgagg gccaataacg gctccattcc gatcttgatg cttaccgcga
    17821 agggagaccc catcgatcgg gtcgtcggcc tggagctcgg cgccgacgat tatttgacga
    17881 agccgttcaa tccgcgagag cttctggcgc gtgtgagggc cattctgcgg cgatcccgcg
    17941 ggatgacgga ggcggaggcc aaccgccgct acgccttcgc ggacttcgtc atcgatctcg
    18001 acgcgcgaac gctttccacc gcagccggag agcccgttcc cctcaccacc gccgagttcg
    18061 acctcctcgc ctgcttcgtg cagcggccgc ggcgcgtgct gtcacgcgag cagctcctcg
    18121 actggacgcg ggggcgagat gcggacccgt tcgaccgcgc catcgacatg accatctcgc
    18181 gccttcgcaa gaagatcgat gcagcagcgc cgggcgtgaa cctcatcagc accgtgcgca
    18241 acaacggcta tctcttcgtt gcgaacgtga agcatctgtc gccatgatcc ggcttggctt
    18301 caccgcgcgt ctcattgcga tcttcgcgac gagcctcgtt gccctccagc ttctcgccgt
    18361 cgtcgcctac gtgatgcagc ggagccgggc gacggagggc agcctgcgtc taccgctgcc
    18421 ggatcaagct gctgctctcg tcgaactcct ggagacgatg cccaaggagc aatggccgac
    18481 gctgctgcga gcggccaaca gcacggacct gagcgttcgc atcttggatg ttccgccaga
    18541 tgatcgggag ccggaatggt acgaggctcc agtcgtcgaa ttcatcctca ggcggtacct
    18601 gtccgtgctt ggtgatcgcg aggtgcgcgt ccgggtggag ccatcgtccg aggagcttgt
    18661 cgggcctctc aaggctctgg cctgggtttc gcccggcgcg gtcgagatcg aggtccgtct
    18721 caagacgggt gagacgctga tcgtttccgc cggtggcatg ctgagcctga gcgtcctcgg
    18781 tctgccgccg ggcttctggg ccggtgtgct gggtctcggg gtcgcggctt tggctctgtt
    18841 cctgttgagg cgggaggccc ggccgctgcg tgagcttgcc gcggccgtcg atcaggctcc
    18901 cgtcggcgag gacgcgcacg tcatccctga tgccccgcgc agcgcgccgg aaatccgcgc
    18961 tctgatcggc gcgttcaatc ggctaacgga gcgcatcgcc agcctcctgc gctcacgtat
    19021 ggccttggtc ggtggcatct cacacgacct cagaacctac gtcacgcgcc ttcggctccg
    19081 catcgacctc atccctgatg ggaccgagaa ggagaaggcg atccgggacc tcgacgacat
    19141 gacccagctc ctcgacgatg cgctgacggc cttcaaggcc ggctcggtcg cccgggagga
    19201 ggagctggtt gatcttgctg acattctggt gcgagaggcc gaggaccgcc gcctggccgg
    19261 ggcctgcgtc acgctggacc tccccgagcc ggtgagggcg gcacaagtcc tgggcgatgc
    19321 gctcgcactc cggcggtgcg tctctaacct tatcgacaac gccattcaat atgggctcga
    19381 ggcgcgcgtc tcggctgtcc tccgcaacca gcgcgtagtg gtgatgatcg acgatcgcgg
    19441 ccccggcatt cgcaccgagg atcgcgatac cgtcatggaa ccgttcgtcc ggcttgagac
    19501 ctctcgcaac cggcgcacgg gcggggccgg gctggggctc gccattgcgc gcggcgtggc
    19561 agaagcccac ggcgggcacc tgaccctcgg cgatgccccc ggcggcggca cgcgcgccat
    19621 catcgagctg ccggtgtttc aggattgagt gctgatcact gactttgagc taagtggcgc
    19681 tccgcttcga gcccgcccac catcgatcaa tcaaagtgga tatggaacac caagacgctt
    19741 ggctttcccc gcgcagccac tattccgccg ccatgacctg gggagcctcg ggctccatat
    19801 cgagcatgcc aagcgcatgg agataggtct cgagaacagc ctcttcctcc tggcggtcgg
    19861 ccttgctttt cttgcgcagg cgcagcacct ggcgcagcac cttgacatcg aaccccagtg
    19921 ctttcgcctc ggcaaacttg tcccggatat cgtccgcgat ggccttcttc tcttcttcga
    19981 gacgctcgag ctgctcgacc aattggcgca gctgctgctg cgccgaagct tgcagcgtgg
    20041 tcatctgctc acccccgtta tggctgactc gaggatcgct tccgtccgtc ttgcgcggaa
    20101 tgcgcgggcc agccgcagct tggttccctg ctctgcctaa tccgcaagcc gagccgccat
    20161 caaggcaaag gaaccgcccg ggaggactta tccccgcaac tcaccagtga ctcgccaagg
    20221 ctaactcagt gactgtgtgg gtgagacttc tcgaaggcag ccttctgctc cggcgtggcc
    20281 tccttttgat atcgggcccg ccactcctca tagggcatgc cgtagatgaa ctcgcgcgcc
    20341 tcgttcttgt ccatcggatg cccggcggca tttgcggctt cctggaacca gttcgccagg
    20401 cagttgcggc aaaagccggc gagattcatc aggtcgatgt tctgcacgtc cgtgcgctcg
    20461 cgcaaatgcg ccacgagccg ccggaaggcc gccgcctcga gctccgttct ggtcttgtcg
    20521 tcgatctgca tgaggtcctc gtccaacgtt gtattgtttg cgtaggatca ggtgattttg
    20581 tcagctggcc tgcgctaacg ggggcttcat tacattgagt tgcaggtccg gtcttgcaag
    20641 cagcccccgc atgatccgca ccagccgctc cgcccaggcg gcctgccctt tcggatcgcg
    20701 gatcaggtcc tggcggacct cgatgatcgc gtgggcaagc ccccgcattg taccatgctt
    20761 ccaaagcgtg tccccttcga gctgcccgac gtacggctcg ttcgctccga tgatgaggtc
    20821 gttctcggcg caaagcgcct cgagcagtgg ctcggccagg cggtcgtcat gatcgcccca
    20881 gaggacgccg gcgtgccatg gacgcgatac ccctttccag ctttcggtga agctgtgcac
    20941 cgaaagaatg gccggggcga cgccactccc gacgcagcga tcgagcacgc gatcgattgc
    21001 ctcatggtac ggacgataat agagccgtac ccgcttttcg cgttcttccg cggtgatgac
    21061 gcgattgccc ggcacgatgg caccgtccga cagccgcatg atgagcgtcg ggtcgtcttc
    21121 gccgcggttc ggatcgatca gcagccgtga gtagcgagtc atgacggccg gcacggccag
    21181 cgcctccgac atgaggcgcg tgacctcggc agccccgatg tcgtaggcga tatgccgttt
    21241 aagctggctt tcgggcaggc cgagcgtccc gtattcgggc gggaacgcat tgccggcatg
    21301 gtcacacagc agcaacagcc ccgcatccgc aggccccggc agaattacga acgagtcgct
    21361 gcaaaccggc tcaatccatt gtttttgcgt cttcatcgcc ttgattgctg ctgttatccc
    21421 tgcaaccgtc cgatcatcga gccttgacgc ggcaaagcca atcccgcaga tgagcgctgt
    21481 cccgatttcg tgtgcggggg gtgcggagag cggggttgcg gtgagagaag accaaaaaca
    21541 cagcaccgaa gagcagcgcc ggactttgct cgaactgtac gacgcggccg tgaagcgggc
    21601 gcacccgtcg cggtgcctcc ccggggcttt gccggaaccg ccgcccaacg gccgcatcat
    21661 aatcgccgga gccggcaagg cgtcagcggc gatggccgtt gctgcagagc aattctaccg
    21721 cgagcgcggc ctgctcgacc gtatcgccgg atttgtcacg acggcgcacg gatacgcgct
    21781 cccgaccgag cggatcgaga tcgtcgaggc aggccaccct gtaccggatg ccgccagcgc
    21841 cgccgctgcc gaccgcgcgc tcgccttggc tcagcaggct ggcgctgagg acatcgttct
    21901 ggttctgctg tccggcggcg cctcggccct ttggtcggcg ccggtggacg gggtgagcct
    21961 ggattccaag caagccctga ccagggatct tttgaaatcc ggcgccccaa ttcacgatct
    22021 caactgcgtg cggaagcatc tctcgcgaat caagggcggc aagctggcgg ctgcggccca
    22081 ccccgcgcgc gtggtgacgc tcgccatctc cgacgttccc ggcgacgaac cggcaacgat
    22141 cggctcgggt ccgaccgtgg gagacccaac gacacttgcg gatgcccgtt ctgtgctgca
    22201 ccggttcgac attacgccgg cgccggaaat agccgccgct ctcgacaatt ctgccaatga
    22261 gacgccgaag cccggggcag ccgtgctcgc ctcgtccgag ttcaagctga tcgcggcgcc
    22321 aagagcctcg ctggaagcgg ctgcggaggt cgcgcggcac aagggctacc gcgtcgaaaa
    22381 ccttggcgat gcgctcgaag gcgaggcgcg cgacgtggca cggtcgcacg ccacgctcgc
    22441 tcgggaagcc ctgaacaatg gcgagcgcgt tgccatcctt tcgggcggcg agctcacagt
    22501 gacggtgcgc ggcagcggat ccggtgggcc gaaccaggaa tatgcgctcg gcctcgtgct
    22561 ggcgctcgat ggcgcgcaag ggatcgctgc gctggcggca gacacggacg ggatcgatgg
    22621 cggcgggggc cgccctgagg atccagcggg cgcactggtg tttcccgata ccaaggcgcg
    22681 ggcctcggcc ctgaatctcg atgccgccac tttcctggcc aacaacgatt cgaccaccta
    22741 ctttcgcgag ctgggcgacc tcgtgctctg cggcccaacc cagaccaatg tcaatgattt
    22801 ccgcgtgatc ttggtcgatc cttagatcca agggtccgac gtcctcatgc cagcaaccag
    22861 cagtttcgga atgagcaaac tggccctatc ctcgccacgc acgtcttccc gcgcccagac
    22921 atacgtgtgc agcctcgcca caggaacgct ggcgtgtctg ctggctttat tcatgacgca
    22981 accggcacat gcggacctca agctgtgcaa tgcctcgcaa agccgtgtcg gtatcgccat
    23041 cggttaccag gacgccaagg gctgggccac cgagggctgg tggaacatcg gagcccagac
    23101 ctgcgaaact ctcctcaagg gagccgtacc gagccgcttc atctacgttt acgcggtaga
    23161 ctacgaccgc ggtggtgaat gggccggcac gaactacatg tgcactcagg acaagtcctt
    23221 cgccatcagg gacgttaccg actgccagcg taggggatac aggcgtacgg gcttctttga
    23281 agtggatacg ggtgacgcca aggattggac catccggcta tccaatcccg aggacgcggg
    23341 agcacaggcc aaatgaggcg taatcgcagg gtcaagatcg tcgcaacgct cgggcccgca
    23401 tcctcggatc cggagatgct ggagcgcctg ttcgagagcg gcgtcgacgt gttccgcatc
    23461 aacatgagcc atacgacgca cgaagccgcg cgcaagctgc acgcgaccgt gcgaaatgct
    23521 gaggtgaagt tcaagcgccc catcggcatc ctgtgcgacc tgcaggggcc gaagttccgc
    23581 atcggcgaga tcgaaggcgg ccgcgtcttt ctcagcgaag gcagcctgtt ccgcttcgac
    23641 acgaacgagg acgtcggctc ggccgagcgc gtgttcctgc cccatccgca gatcttcgag
    23701 gcggtcgagc ctggacacac cctgctgctc gacgacggca agctgcgcat gcgcgtggtc
    23761 gaccgcagcg tgcgtgagat caccgccgag gtggtggtcg gtggcgcgct ggcaagtcgc
    23821 aagggcatcg ccacgcctga cagcgtgctg cccatcaacc ccatcaccga gaaggaccgc
    23881 gccgatctcg actacgcgct gcggctgggc gtcgactgga tcgcattgtc gttcgtgcag
    23941 cggggcgagg acctcgtgct tgcgcggcgg ctcatcggtc aggcagcgtc gatcatggcc
    24001 aagatcgaaa agccgtcggc gattgccgat ctcgacgaga tcatcgccaa cgccgacggc
    24061 ttcatggtcg cccgcggcga tctgggcgtc gaaatgccga tcgagcgcgt accgggcttg
    24121 cagaaggaga tcacgcgaaa ggcgcgcgct gccggcaagc cggtcgtcgt ggcgacacag
    24181 atgctggaga gcatgatctc ctcgcccgtc ccgacccgcg cggaggtctc ggacgtggcg
    24241 accgccgtct tcgacggcgc cgatgcaatc atgctgtccg cggaatccgc ggtcggtcag
    24301 tttccgatcg aggccgtgcg aatgatggac cgcatcgcga tccaggtcga aaacgacccc
    24361 ggctacgacg acctcgtgca cgctacaccc atcgttccgc tgccgacggg agcggacgcc
    24421 atcgcagcgg ccgtagagaa gattgcgcga acgatgcggc tcgcctgtat catctgctac
    24481 acggccacgg gatcgacggc gctgcgggtg gcgcgcgaac gtccgggtct acccgtcatc
    24541 gggctcactc cggtgctcgc caccggacgg aagctcgcca tcgtctgggg cattcattcc
    24601 gtgctcaccg gcgatccggc caaccttgcc gacatggtcc gcaaagcctg caagatcgcg
    24661 ttcgaagatc agttcgcatc cccgggcgat ggcgtcatca tcacggcagg cgtcccgctc
    24721 ggcacccccg gcaccaccaa catggtccgc gtggcctatc tgaacgagtc gggcgcgccg
    24781 atggccgagc aggtgtgagc aggtgtaagt ggctggcgcg gagcgaaggg gtggtatcag
    24841 atcagatacc ttggcctttg acttctcgct cgagctcctc gacggccttc tgagcgccct
    24901 cggcaaacgg attgacttcg agcagcttcc gataggcagc cagagcgcct tccttcgccc
    24961 cgtactcgcg gaggatgtgc cccaaagcgc taagagcacg gaaatggttg ggatcgagcg
    25021 ccagggcgcg gcgcaggtcg cccagcgccc ggtgcacctc gccctccagg tacaggacgt
    25081 gcgcacgctg gctccagcct tcggcgtaat ccggcgcctg ctcgacgacg gcatcgagca
    25141 ttcgcaaggc gagcctcggg ttcttgtcct ccatggcctt ggcggcgcgg gccatgagca
    25201 ccgtgaccgt atcgctgcct gagttcagcc agagcctcgc gatggccttg gaggtccgct
    25261 cggccgcctc ctcattctca gccgtcgcca acagagcgta aagattgtcg agcatcttcg
    25321 cgcgctcgac ggcgctgtcc ggcagcttct ccctcagctc cggggcgtcc gctgcatccc
    25381 cttggagttc atcgtccggc tccgggaggt tcggaatttc cggtgaggtc tcgggagcgc
    25441 caggtgcctg agctcgagat accgacggga aaacggcaac ggcgcccagc aggcaggcgc
    25501 ctgcggccat tgcgatcagg cgatgtgcga gcaagttgcg catgccccca agatagggtg
    25561 agctttgccg cccgtcaaag cccaacgagg ctgcgccgcg atcagccctg gcgcgccttg
    25621 aagcggcgtt gggacttgtt gatgatgaag acgcggccct tccggcggac cagacggttg
    25681 tcacgatggc gcgaccgcag ggatttcagc gagttcttga ctttcatggc acgtccgtgg
    25741 ccctcgggcc caaggccacg caggtcctct gttcgatcag aaatagagaa acggcgggac
    25801 cgggcccgcc atagtgcgtc gtgggacaag gaccacaaaa ggcggcctct cgtcaagtca
    25861 tctgtggccg cggatgcggg ccagccccgg atgtgaatcg ggactgatga gccagtgatt
    25921 tcagctgttc gcccgcgcca gcacggcctt gagcagcacg ttgacgcctg gaatggtggc
    25981 ctccaagtcg atgtcctcac gctcgttgtg cgagatcccg cccttgcagg gtgtgaacag
    26041 catgaccgtc ggacagacgt tcgccacgtg ataagcatcg tggccggcct gcgagcgaat
    26101 gtccatgaat gggacatcga gctgccgcgc cgtcgtcctg acgagatccg ccagccttgg
    26161 atcgaattcc aatccgccga agccccattg ctcggctatt tcgatctcgg tccgcgagcg
    26221 gcgggcgctt tcagccacgg ccgccttcac ctcgcgctcc atgtccttca gccgcgccgg
    26281 cgagggatgg cggaagtcga catagagctg tgcctcatcg gaaaggatgc caggcagatt
    26341 gggcgcgaga tccagccggg ccgcggtggc cttgccatct tcgggcgcgt acctccagcc
    26401 gatgtcatcg atggcaacga ccaggtgcga cgcgccgacg agggcattgc ttcggaggtg
    26461 catgggcgtc gggccaacat gggacgtctc gccgcgcacc acaatgcgca tgccgatcat
    26521 ctgatagcca cccgtcacca cacccagcgg gacgccggcc tcgtcgagct tgggcccctg
    26581 ctcgatgtgc agctcgaagt agctgtcgag cgcgcgccca cccacagggg cctcgcccag
    26641 atagccgatg cgtgccagct cgctcccgaa gctcagccca tccttgtcgc ggcggtcgtg
    26701 cgcccaggcg acgctctcgg cgccggagaa cacgagcgag cacatcatcg gcggctgaaa
    26761 acgcgcaccc tcctcgtttg tccagttgac gacctcgatg gcgcggcgtg tctggatacg
    26821 cgcctcatcg agcgtacgca ggatttcgag gcccgcgagc acgccgagta tgccgtcgaa
    26881 gcggccaccc ttggcctgcg tgtcgagatg actgccaacc agcacgggcg gcagctgagg
    26941 ctcggtgccc tcccgccgga cgaacatgtt cccgagctgg tcaatgcgga gcgcgtagcc
    27001 gtcctcattc acccactgcg cgaagagatc gcgcatggcc ttgtcctcgt cagacagggc
    27061 aaggcgccgc aacccgccgc gctcggtgcg cccgatctct gcggatgcca tcagcgtgga
    27121 ccacaagcgc tcggcattaa ctgcaagttc gaccatggct catcagaccc cgttcaagct
    27181 ggtccgccca ggatagcagg gcaagcgccg attggctgcg cggatctgca gagacgcggg
    27241 cgcgtgatca caagggcctg gcaggcgggg caactcgaca gcgggtggct tctgcaatgt
    27301 ttcagccaac ttgcgccccc tgcgtggctc acccataagc tccgtcaccc cgaaaccgga
    27361 gaggtggatc atgaacctgc aggatttcac gaccctgacc ttcgactgct acggcacgct
    27421 gatcgactgg gaaagcggca tggtggaagc gctgaagcca ctgacgagcc gggtcagccg
    27481 gccgctgtcc cgtgacgaca tcctggaagc ccatgcgcgc caggaatcat cgcagcagtc
    27541 ctggacgccg gccaagaggt acagcgagct gctggccgtc gtctacaagc gcctcgcaga
    27601 ggagtgggga gtcgccgtga cgtgggagga gtgccaggct tacggcaact caatcggcaa
    27661 ctggccggcg tttcccgata ccgccgaagc gctgcgctat ctcaagcagc actacaagct
    27721 cgtcattctt tcgaacgtcg acaacgccag cttcgcgctg agcaacaaga agctcggcgt
    27781 tgatttcgac gcaatctata ccgccgagga catcggctcg tacaagccgt cattgcgcaa
    27841 tttcgagtac atgatcgaaa agctggaggc gctgggtgtc gcgaaatccc agatcctgca
    27901 cacggctgaa agcctgtttc acgatcacct gccagccaat caggtcggcc taagatcgtg
    27961 ctggattcat cgcaggcacg aggcgggcgg cttcggcgcg acgatgaaac ccgagaccgt
    28021 cccgaagtgc gatttccggt tcaccagcct cgctgcgttc gttgaggccc acaagcaagc
    28081 tacgggaagt tcagcgtaag agctgctccc ttaggagccg ggtgctgggg ccagtgagga
    28141 ccagcacccg agctcaccgc tagccgcgca accgcgtcct cacgatgcgc gggaattcat
    28201 ccccgtccgg gagctcgcca gcgggcgccg actcctgcga ggaggcgcgc tgtgaacccg
    28261 cctgatcgcc ttgagtcgcg cgcctttcat cagcgcccca ccagcggtcg ctctgcgcct
    28321 ctctcgcgat ggaggctggc tgctgcgtgg atgtgtgggt cagagattgg ctggacgttt
    28381 gcgaggaagg ccgtgctgcc gcgggagctg agctcgcacc cgtgaaccag gcatcgatca
    28441 ggtcgatttc gtcaggcgtc agcttgagac cgtggctacg gcaccgcgcg acgacgaagt
    28501 tccggaagcg gcctgcgaac aggttggccg atgcgccctg ctcgaaccac ggatcggcct
    28561 cgctgaccac atcgagcgcg aagcggatgt catcgcgccg gatatcgaga cccaactcgc
    28621 gcgcacggtt ggcgacattc gtgatcgtct gcgccccctg caagccactg ctgttgatct
    28681 cgttcgcgag catttcgaac agggccctgt actccggcgg agacaacgga ggagcctggc
    28741 aggcttcgtg aatgcgcgcg atggactgct gaagcgcggt ggcggtatcg gcggtgcgcg
    28801 ccgccggtaa ggtcgtagac ggaccagggg cggaaagtcc gcgctgtggc gcagcaggcg
    28861 tgatctcgac gctgctttcg cgcaactgct cggagagcgg cgtgctgctc gggcggtagc
    28921 tcggttgcga tgattgcggc tggctcgaga cgatccgctt tgccgccgcg agcgggtcga
    28981 gggcctgggt cagtgcggca gcagcagcta ccgccgcaga gcgcggctgg cgctgcgggg
    29041 acaacgcggc tggccgctcg ttgcgctgtt ccgcacgcga tgacagcaac gcttcttcgg
    29101 gttgcgactt ccgcgtcgca tcatagacga cgtacggcgg ctgctcgctg agccggatcc
    29161 cctccggcag gctttggagc agcaggtcac ggaagctgcc ggcacccgcc cagttcgttc
    29221 cgacggtacg gtcatgtccg agcgcccggg tcgcccgctc cgcaagggct tcgagcggga
    29281 ccggcgagcc ggaactgcgc accgcaccag tgatttcggc catgatctcg cgacgcagat
    29341 caccaccagc gcctggcgtg tgatcgtcag cccgcacact gctgccaaga atgacgttca
    29401 tcaggtccgc ttcgcggact tcgccgtctg cgatcgccac gtaaggggcc gccgtgaagt
    29461 cgttggcgaa cacgaccgtg cggcgggcat gcgcacgcag gcggtgcagc acgggcgtga
    29521 aatccgcatc ccctgagagg atgatgaact cgtcgaagta cgtctcgtga tggaggtagt
    29581 cacggacgtc catcaccatg cggatgtcgg acgaattctt gagctgggcg gtcagcggtg
    29641 gacaatcgac cacctcgaag ccggcgcgca gaaaatggtg ccgcacgaac gggaaggagt
    29701 tcatgtccgt cgagttgtcg ttctggttcc ggcgcggcac cggattgccg tagcaccggt
    29761 tcaggacgat ccggcgctgg acgttgctgg gcagcttgtt ggtcggcgtg atgaggcgtc
    29821 cgctctcgat ctcagccagc cacacgctgg catccttggc aaaccgcttg gcggcttcct
    29881 cgctcctgcg tttcagcgac agatagatgt tgtcgtaatc gacgaacacc gcactgagta
    29941 tctgccctga agtctcacgc ttcattcgta agcccccgct gcccgccaca tttacccatt
    30001 gattcgcaac tctttacgag tcgcaggggc accgcaagcg caccagggtc gctcatccca
    30061 accttcgaga tgagcccttt gaaatcaatt gtttacctga ttttcgagcg tgcaagcttc
    30121 gtggataacg tccgaggatg gatacggggt gagatccggt tggtaagaac ttggcaagaa
    30181 cgtgaaaacg cgaggcaagg aagtcacgag cacgaggcga tctgcgcgaa cgcgcaagtg
    30241 ccgattccac gagttggcga cttcacgatc tcacaaattc acctcgtcct caggcgcggt
    30301 tcttgcgctc acgaatctcg gcgaaaacct tccagtccgg ctcgccttcc atccccagcc
    30361 tcttgcggat ctccgggctg ttcggccgca ggaacggatt ggtcgcgagc tccaggtcaa
    30421 tccgcgtcgg cgacgtcggc tttccttcag ctctcatctt ttcgacgtcc ttcatccgct
    30481 gctgcagtgc agcgttctca ggctcgatcg tcagggcgaa tttcgcatta gacagcgtgt
    30541 actcgtgccc gaagtagact tgtgtcgagg gcgggagggc catcactttc tcgagcgact
    30601 gccacatgac ctcgtagggc gcctcgttca cgcggccgca gccgatggcg aagaggacat
    30661 caccgacgaa tgcgacatcg gctgccggga tccagtagat gatatggccg gcagtgtggc
    30721 ccgggcagtc gaacacgtgc acctggaacg agccgaacgg gaacacgtct ccctcaccga
    30781 cggcctgatc cagcccggga atcttggcag cctcggcacg cgggccgatg atggtgcagc
    30841 ccgtctccgc cttgagcgcg aggttcccgc cggtgtggtc cgcgtgataa tgcgtggtaa
    30901 ggatatgtgt gagccgccat cccttctcgg caagcgcgcg tttcacggcc tccgcgtccg
    30961 gtgcgtcgat ggaggcgacg acatctgcgt cggcatccct gatcaagacg ccgaagttgt
    31021 cgctgagaca cgggaactgg tggatttcga gctttgccac ggctgaagct ccatattgat
    31081 cgatggtcgg cggcctgcag cgcaacctag cagcaggttt cgggattgca accgccaccc
    31141 ctctataccg aaatcatgag caatggtttc ggcgggcaag caggtagcca acggatcata
    31201 tgaattgtga cgttgccgaa ctgagaaact tctataaccg gccgctcgga tcgatggtgc
    31261 gacggctgct tgcgcaccgc attcgggctc gctggcgccg tttgcagggt gagacgctta
    31321 tcggcctggg ctttgccacg ccgtaccttg gttcctttcg cggagaggcg ctgcggatcg
    31381 gagccctgat gccggccgca cagggagcgc tcgtgtggcc cgacattggc gccaagatga
    31441 gcgcgctcgt cgacgaggag catctcccgc ttccggacag ctcggtggat cggctgttgg
    31501 cggtccactg tctcgagacg gccgaccggc cgcggacgat gctgcgcgaa atctggcgcg
    31561 tgctcgctcc ccagggccgg ctgatgatgg tcgttccgaa ccgcagggga gtttgggcgc
    31621 ggctcgaccg cacacccttc ggccagggac gcccctacag ccgcacccag ctcgaggatc
    31681 tcctcagcga agccatgttc gcgccgctcg agtggtcgca ggcgctctac gtgcccccgg
    31741 tcgagtcaaa gatggtcata cgctcggcaa ccgcattcga gcgcgtcggc gcccgactgt
    31801 cgcgcgggtt cggcggcgtc atccttgtcg aagcccgcaa ggagctgacc gttccgatca
    31861 tcaaaaaggc accggcccgc gtgctcggcc gattggtcac cgctcccggc ggtgccaacc
    31921 ccaggtccga gacggaggaa aaacggatcg ccggtccgga tcagatcgcc gaccggcgct
    31981 tcaggagaaa tacggccttc tcgccaaaaa tgttctgcac cgtcagcggc acgcggggcg
    32041 agagctgacg gcctcctgcg ttgaaggccg cggaacgctc gacggtcgca ttcaccttcg
    32101 tgcagagttc tgcgaagtcc cggatcgtgc acaggtgagc attgggcgac tcgtaccacg
    32161 gctcgggcag gttctccgtc tgcggcatcc gtccggtgaa cagcagccgt gtccgaacct
    32221 gccagtggcc gaagttcgga aacgagacga ttgcccgccg tccgatccgg agcaggtttt
    32281 gcagaacggc cttcggccgc agggtcgcct ggatcgtcag gctcaggatg gcgtaatcga
    32341 aagcgtcgtc aggataatcg tcgaggtcct ggtcggcatc gccctggatg acggacaggc
    32401 cgcgcgccac acaggcattg acccgctcgc gctcgatctc gatcccgcgc ccgtcgacgc
    32461 ctttcgtatc gaccaagagt tgcaggagcg ccccatcccc gcagccgaca tcgagcacgc
    32521 gcgcgccctc ggtcaccatc tcggcaatga ggagatggtc gacacgggcg gtgacctgca
    32581 gcggctgttg cgatctgagc atgcggctga tcctaacccg aaagcatcag cccgcgcgtg
    32641 ccggcgagat gccgcgcttg gccgccgccg agttgataaa gccacggacc gtcgcaaaca
    32701 gttccggctc gtccagcaga aaggcgtcgt ggcccttgtc gctctcgatc tcgacgaagg
    32761 acacgttcgc cgcgaccgca ttcagggcct gcacaatctc cttcgactcg cgcgtcgggt
    32821 agagccagtc gctggtgaaa gagacgacgc agaaccgggt cttcgtgccc ttgaaggcgt
    32881 tggccagaac atccccgtgc tcagcggcca gatcgaagta gtccatcgcc cgggtgatgt
    32941 agagatagct gttggcgtcg aaccggtcga cgaagctgat accctggtga cgcaaatagc
    33001 tttccacctg aaaatcggcg ctgaacgaga acgagcggtc ggtccggtcc tgcaggttgc
    33061 ggccgaactt ccggtgcagg gcttcctcgg atagatacgt gatgtgagcg gccatgcgcg
    33121 ccacggccaa tccgcgccgc ggcacgcgcc cgagctcgag gtagcgtccg ccgcaccagt
    33181 cggggtccgc catcaccgcc tgccggccga cctcgttgaa ggcgatgttc tgcggggaat
    33241 gccaggcggc cgtggcaatg ggtaccgcgg tgaacacgcg ttccgaatgc cgtgccgccc
    33301 actccagcac ctgcatgccg cccatcgagc cgccgatgac gcagaagagc tggtcgatgc
    33361 cgagcctgtc gatgagccgt acctgggcat cgaccatgtc gccgatcgtg atgacgggaa
    33421 agctgaggcc gtacggtttg cccgtcttcg gattgatgga ggccggccca gtcgatccca
    33481 tgcatccgcc gatgatattc gggcagatga cgaagaagcg attcgtgtcg atcggcttgc
    33541 cggggccgac catcgtctcc caccagcccg gcttgcccgt gaccggatgc ctgttggcca
    33601 cgtgctggtc gccggtgagc gcgtggcaaa ccagtatcgc gttcgacttg tcggcgttga
    33661 gctcgccgta ggtttgatag gcctgggcaa atggcaccag agcctgcccg cagtcgagca
    33721 gcagcggctc gttgaattcc aggagcttgc tggacggccg ctgggcttcc gacacgcggg
    33781 gctcaatccc gcggtcgaac tcctgcggat tgaccgtgtt catggcgcaa ccctcgacag
    33841 tgctgccctg cggatctcat gcattcacgg cagaggctat gcggcgcata gccccgaacg
    33901 ccgtgattgc gctagctagg gcctcgcccg agaaagttca atcgtttgtt tgctttcggc
    33961 gggcagcacg catatcaatc gggccgattc agcatcagaa agcatcagac gcgtcatggc
    34021 cagcggaatg ccgagcccta agcggggcgt gatggagatc gaagcctatg tgccggggga
    34081 aagcaaggtg cccggtggcc tcgagccgat caagctgtct tcgaacgaga acccgctggg
    34141 tgcgagcccg gccgccgtgg cggcattcaa agctgccgcc gacagcctcg agcgataccc
    34201 tgacggcagc gcgacagagc ttcgcgaagc catcgcgcgc gtccacgggc tcaatccgga
    34261 ccgcatcgtc tgcggcgcgg gatcggacga gctgctcaat ctgctggcac acgcttatct
    34321 ggagcccggc gacgagacga ttttcaccga gcacggcttt ctcgtctacc gcatcgccac
    34381 gctgggctgc ggtgccaaac ctgtcgtcgc gccggagaag gacctgcaca ccgatgtgga
    34441 cgcgatcctc gcgcgcgtca cagcgaagac gaagatcgtg ttcatcgcca atccaaacaa
    34501 tccgacgggc acgtatattc cgttcgacga gattcgccgg ctgcgccagg gtctgccgga
    34561 tcacgtgctg ctcgtgctcg atgcggccta cagcgaatat gtgaggcgca acgattacga
    34621 ggctgggatc gagctcgtcg cgaccaccgg caacacggtg atgacgcgca cgttctccaa
    34681 gatccatggc ctcgcagcgc tgcgcctcgg ctgggcttac tgcccggagg cggtggccga
    34741 cgtgctcaat cgcatccgcg ggccgttcaa cgtatccgcg ccggccatcg ccgcaggcgt
    34801 cgccgccctc ggcgatcgcg gtcacgtgga gacttcggtc gcgcacaatg agaagtggct
    34861 tccctggctt acggccgagc ttgaaaagct cgggctggcg gtgacaccga gcgtcgccaa
    34921 cttcctgctg gtccatttcc cgaaagagcg cgggcgggat gcagacgcgg cgaacgcttt
    34981 cctccagtcc cgggccatca tcgtgcgcag gatgggcgct tacggcctgc ccggcgcgct
    35041 caggatcacg atcggcaccg agtcggagaa ccgcgccgtg gtcgaggcgc tgaccgcttt
    35101 catggctggg acggcgcgat gagcggccca ttgttccagc gggtcgccct gatcgggatc
    35161 gggcttatcg gctcctcgat cagtcatgcc ctgcgccgcc gcgggctcgt cgaaacgatc
    35221 gtcggcaacg ccaagacgga ggcgacgcgg gcaacggccc tgaggctggg tctagtgtcg
    35281 gctgcgtacg agagcccggc cgatgcggtg aaagaggccg atctcgtgat cctctgcgtg
    35341 ccggtgggcg cttacggagc catcgcgcag gagatcgcgc cgcacctcgc gccaggagcc
    35401 attctgacgg atgtcggctc ggtcaaaagc gcggtgatcc gcgatgtcgg tccgcatgtg
    35461 cccgctggcg tgcacttcat ccccggccac ccgattgcgg gcacggagca ttccggcccc
    35521 gaggcaggct ttgccgaact gttcgacaac cgctggtgcg tgctgacacc cccgaacggc
    35581 gtcgatgcag aggcgctcca aaagctgcgg cggctgtggg agggcatggg cagcaacgtc
    35641 gagatcatga cggccgagca tcacgacctg gtgctcgcca tcaccagtca cgtgccgcac
    35701 ctcatcgcct acaacatcgt caacacggct gcccacctgg aacgggtgac cgacagcgag
    35761 gtcatcaaat tctcggccgg cggctttcgc gatttcacgc gcatcgcctc ctcagacccg
    35821 acgatgtggc gcgacgtgtt cctcaacaac aaggaagcgg tgctggaaat gctcgggcgg
    35881 tttacggagg atctcacggc tctgcagcgc gccatccgtt acggggaggg cgatacgctg
    35941 ttcgagctgt tctctgccgc gcgtgccatc cgccgcggaa tcatcgaggc tgggcaggat
    36001 acgccggcac cggacttcgg ccgccggcag gcgaaagcgg cgctcggcgg gggcggctcg
    36061 cagtcgtgaa agaggcgggg tccagcagga aaacccacga cctgtgggtg ttcggctacg
    36121 gctcactgat gtggcggccg gggttccaat tcgaggaagt ccggcgagcc accctcgtcg
    36181 gctaccggcg ctgcttctgc ctctactcca cccatcacag gggcaacgag gagcgccccg
    36241 gtctggtcct gggcctcgat cgcggaggta cgtgccaggg cctggccttc cgcgtgcctg
    36301 cggagtgccg actggaaacg ctcgagtacc tggccgcgcg cgagcaggtc agcggagcct
    36361 accgggacac cctggtgccg gtgacactcg agggcgagcc gcggcaggac gtcttcgccc
    36421 tcgcctacat cgtcgagcga cggcatccga gctacgcggg ccggctgagc cttgccgatc
    36481 aggcaaggct gatccgcggc gcatgcggca tctccgggcc caacctcgac tacctgatca
    36541 acaccctggc ccacctcgac agcctcggca tccacgagcc ggagctccgc cgcctgctcg
    36601 ccgtgatcgg agcccatttc gcacgcggtc gtttcggcaa tggcggcagt accggcgcgg
    36661 cagccctgct caagatctgc cgccatttgc cggtcgacgt tcctcacctg aagcttggag
    36721 agaggcggcg cttcatccac cgccggcaga tctcggcctg ggcggcgcgg agccgttcga
    36781 cgtaggacag ttcgggcctg aagcgcctca gtgccgcata gatgatgtcc gctcggcgct
    36841 gagctcggaa aaatgcgccg tgcgaaggtc gagctcgacc cggcgacgct cgcgcgcgct
    36901 gccggcgaga aaatggacgt ggacctggtt ggccccgagc tgcgccccgc gatggcggat
    36961 ttcggcaaga ttgagggatg gagcgtcatg ctccgtccgg ccgaccctga ggacgatgcg
    37021 gcgcccgttg ccatcgcaac gcacgagcat gtagacggct ttcggaagct ctggacagcc
    37081 gatcaggctg aaaacggaat gcacgtaacg ctcgcccgac gctcctgccc agaactgaaa
    37141 ctgctgggca gccggttcat ttaaaagatc atgccccggt gaggctgcgg ccgcccttgc
    37201 ggcggcaatc tgtgacagct cgacaatacg cgcactcatc ttcaaaccct tcggcgcctt
    37261 acactgccgt tatcaccgga ccatagtaaa cgtgacgtgc attttcagca gaagttaggc
    37321 tcaatcacag acatttggcg gcgttgtgaa ggtgaaatgt gtccctgtac agcaatgaac
    37381 ggccgtacgc tcgcctggtt cccactcgtg gctcgcttgg ttcccactca tggaatgcgc
    37441 aaggggcagc cagaccacta cggccgcata gcacttgacc gttgaacgat tggtaatcga
    37501 cgatccggga aagggctcac actaacgcgc gggttgtcac tacaggaaat gcattagccg
    37561 cagcgcgagt atccgcagtc ccggcacacc caacagcctt cctctcttac gaaggcaagc
    37621 gagttgcatc gcggacagga gcggatccgc tgaccagcgc tccctgcgta gcctaccgtg
    37681 ccactgctgc tcggcgcgct ttctatttct gcgcccgctt gcatcagctc gatgagcgga
    37741 gcgatgtcct cacggcgctg gagaaagccg gtccgcacca tgtgcgtctc gatcacgccg
    37801 ccaatggcgg cgagcaggct cggcacataa cgcccgccca tccattgccc gccctgtgga
    37861 tcgaagaccg ccttgagctc gtcgacgacg aagctgacgt cgccgccacg ccggaaaacg
    37921 gcactgatca tgcgggtgag cgccaccgtc caggcataat gctcgaggtt ccgcgtgttt
    37981 atgaaaatct cgaacggacg tcgccgccca tcgtgctcga tgtcgttgat cgtgatgtag
    38041 atggcgtgat cgctgcccgg ccatttcagc ttgtaggtga acccggcaag cgcgggctcg
    38101 cgctccagcg gctttgccat gtagacgatg ccatcctccg agctgcgcgc gagctgccgg
    38161 tgcacggctg acggttccag cttgccaggc tccttcgccc cccgcgcgcg ctcttcggcc
    38221 tcccctttcg gctcctccga cattttgcgg ctcgaaagca cggcacccgt aaccgggttc
    38281 gggcggaatg tcgtacagcc cttcaggccg agatcatagg catcgagata gacgttctgg
    38341 aactcctcga agctcatgcc ggcggggcag ttgatcgtct tggaaatcga gctgtcgaca
    38401 tggcgctgca gcgcggcctg gatctcgagg tgggcggacg gcggcagctc ggcggtggtc
    38461 acgaacgagt ccggcaaagg aaccgagtcc ccgaagcgcg agcggaagat cgcgtgcgca
    38521 taatcctcca ccatcatctc tcgatggctg ccgtcccgct ccagaacacg gcgcttgtaa
    38581 aggaaatcga acaccggctc gacgccgctc gagacgttgc cggccagcag cgaaatggtc
    38641 ccggtgggcg caatagaggt gaggcagccg ttacggatgc cgttcgtggc gatggcctga
    38701 cgcacctcgg gcgcgaggcg cgcaacgccc ggacgcgcca ggaacttctc agcgtcataa
    38761 aggggaaagg cccccttctc ggccgcaagc tcggtgctcg cgaggtatgc cgcccgctgg
    38821 acatgcgcca tccaggtctc ggccaaagcc aaggcccgcg gcgagccata ccgcgccccg
    38881 gcgaagatca gcgcgtccgc gagccccgtc acaccaaggc cgatgcgccg cttggccagg
    38941 gcctccttgc gctgcgcttc gagcgggtag ttcgatgcat cgatgatggc gtccagcaag
    39001 cgtaccgcga tgcgcacgcg ttcttcgagc cgggggagat cgacgtcggc ccccgcctcg
    39061 aacggcttgt cgatcagccg ggcaagattg atcgagccga gaaggcaggc gccgtaaggc
    39121 ggcagcggct gctccccaca cgggttggtc gcgtaaatct gctcgcagta ggcgagatta
    39181 ttctggtcgt tgatgcgatc gatgaaaatg acgccgggct ccgcatactc gtatgtggcg
    39241 cgcatgatgc gctcccacag gtcccgagcc ctcaccgtgc ggtacacccg gccatcgaac
    39301 acgagatccc agggccggtc ctcgcgcacg gcattgatga acccgtccgt caccagcacc
    39361 gagagattga agttgcgcag gcgggcaggg tcggccttgg cggccacgaa ctcctcgatg
    39421 tcggggtgat cgcacctaag ggtcgccatc atcgcgccac gccgcgcgcc cgcggacatg
    39481 atggtgcggc acatcgcgtc ccagacgtcc atgaagctga cgggaccgga cgcatccgcg
    39541 ccgatgcttt tgacaagggc ccccttggga cgcagggtgg agaagtcgtg cccgatgccg
    39601 ccgccttgct gcatcgtcag cgccgcttcc ttgacgttct cgaagatcga actgaggtcg
    39661 tcctcgatgc gccccatgac gaagcagttg aagagcgtca cctggcggcc gctggcggcc
    39721 ccggcgatga tgcggcccgc cggcaggaac tcgaagctgg acagcgcctc gaaatagcgc
    39781 tcggtccaac gctcgcgcgc cttcctgccg cccttctcga cggaggctgc cgtgcgcgcg
    39841 acccggcgga acgtgtcctc cagcgagcgg tcgaccggtt cgccgtcagc cgacttcagc
    39901 cggtacttct gctgccaaat atgttccgag atcgggctga tgctgaacgg gtctggatgc
    39961 atgacggact ccgttgggac ctcatgagac tagcccgctc accccatccg cgtcaacaat
    40021 ttgttccgtt aatgttcctg ttttgtgtca gccattcaag ggccaccggc ggcggatggg
    40081 gcgcccgccc cgcctcttcg atcaggcggg cgcaggcccc ctcgagccgc tcgaggagca
    40141 aagccttgaa ttccgcccgc ttcaggcccg ggggaatggg ctcgaggatc tcgaccacga
    40201 tcgtccccgg ataacgcttc aggctccgcc gcggccagaa caggcccgaa ttgagcgcca
    40261 gcggcacgca gggaattccg agcgactcat aaaggagcgt cagcccgggc ttgtagtccg
    40321 gcggcgcacc gggcggccgc cgcgtgccct ccgggaagac gaggatctcc cggccggcct
    40381 ccgcccgtgc gcgcgccgcg tgcagcatct ggcgcaatgc agccgccccg gccgagcgcc
    40441 ggatgggaat catgccgaac ttgcgcgaaa accagccgta gagtgggatg agcatcagct
    40501 ccgccttcat caccagcgcg ggatcgcgga acagcgggat gagtgcaaag gtatcccacg
    40561 ccgactgatg cttggctgcg accaggatcg gcccaggcgg caggcgttcg agccccctca
    40621 cctccatgcg cgttccgacg atggcctcga gcagccagac gctcacgcgg gcgtgcagct
    40681 tcagcgccca cattgcccag gagcgcgggc cgaacaggag tgggctgccg atcagcagga
    40741 aaagagccgt gttggcataa aagacagcaa cgaagaggag ggagcggatc atcgccgaga
    40801 cttctttcag cgcatcacga gcggtcggac ggctccgccg tcgccagcgc ccttggctcc
    40861 attccgtcga ccagccgaag gacggcataa cgagccgcag agggcaggaa tttcaagtat
    40921 tccgccagga gcagacggat cagccctggg ctcttccacc aactctcttt ctgaaatgga
    40981 cctgtgacgg gatacggaat gagcgcgaca tccggcatga cccgcccgag ctcgaccagg
    41041 ctgcgcggca tatgatagct cgaggtcacg acgatcaggg tcgagaagcc gtgtttcgct
    41101 gcccaggctt gcgtctcggt ggcattgccg atggtgtcct gggcctcgta gccgaggtcg
    41161 acgcagcaat cgaacaggcc ttcgccgagg ccagcgagtt gccgtatctc tgcgccggtc
    41221 gtctggcggt tgacgccggt cacgagcagc cttttgccga ggcccttcgc caaaagctgg
    41281 ccagccgcag atatccggct ttgcgtgccg gtgagcgcaa caataccgtc agctttctgc
    41341 accgccgaag gtgccggatg catggcggat cgggcaaaaa ggaaaaagcc aaccaccagg
    41401 gctgccacga gtaggccaaa tccgaatcca agccgccatg ctcgccgcat cgtcgtcatc
    41461 aagaaacgtc catccacccg ccggcttcgc ccgccggcat aacgggcctc cccatatcgc
    41521 gacgcaacct aatccattcg gccgagaaag cgactatgac agcggcatgc atggatcttt
    41581 ggtgattttg gccttaccgg gctgttactg gcgcgcatgc aatatgcgat aaacaccgaa
    41641 gcgcgatgtc agcatgcaca gcccggcgat caccaccacg acgaagccga gcagaacgta
    41701 gccgggcgta tccagcgctc ccgtgccgac caggcgccgc atttctgcaa tggttacagt
    41761 tcccccgccg agcagttcca tcatggccgg catcgtgaag aagacggcca tcgcacaaat
    41821 ggccccgacg aagccggcac gaatgccgag cgacaaaaag tggcgctcga actcacgtgc
    41881 aatgaaacgg tcggtagcgc cgacgagatg cagcacctcg acgatctcgc ggttggacgc
    41941 cattgcgctg cgcgttgccg aaatgatgat cgcagtcgta gcagaggcca ccagcagcag
    42001 aatggcgaga ccgccgagcg caaacgagcg cgtcacggtg cggatctgct gctgccagtg
    42061 ccggtgatcg tcgagcgtga cgcccttgaa ctgcgtggcc agagtctgcc gcaacgcagg
    42121 gatatccggc ggattggtgc ggtcgagctc cagcgcaacg agacgcggca ccggcaaggt
    42181 gctcagaatg tcgagctggc cgagccacgg ctcgagcagc gcattcgatt cgtcaacgct
    42241 cagcgcgcgc gccccgcgga cgcccggctg gcggttcagg aaggcgacga cgtcgttcag
    42301 cgcaccttcc atgttcggga tgtctttggg ctcgagctga accgtcacct cgctggcaat
    42361 gtccctcagc caggccgcgg cggactggtt catcatgtag acggcgcccg cggtgaggca
    42421 ggcgaagaag cacatgatcg agacgacaag ggtgagggag cgaccggtca ccgagcctgc
    42481 gggcacgatg ggggtggtcg ccggatcgcg attggcggcc cgcgccggcg catacgcttc
    42541 gcggcgcgtc gagacggtcg ggctctgtgt caccggctcg aactggggca cgtggggcgg
    42601 accggtcggc gcgtctgcga cctcgtaggc gccgaagtca ccggacgccg agccgaagcg
    42661 cgacgaggcc gtcggtctgt catacgatcc tgacatgccc ctcgtgtagc tccagacgcg
    42721 gcgccttgta ctgccgcatc agttgatgat cgtgggtggc aatcacgacg gacgttccca
    42781 ggcggttcag ctcgatgaag agcctgagca gccggcgcgc catttgcggg tcgacgttgc
    42841 cagtgggctc gtcggccagc agcagctcgg gcttgcctat cacagcgcgc gcgatggccg
    42901 cccgctgttt ctcaccgccc gacaaaaccg acgggaacgc gtgcatccgc tcaccgagcc
    42961 ccacccactg cagcagatcg gtgacatcct ccttgtagtc ggaaacgcgc ttgccgacca
    43021 cccgtaacgg cagggccacg ttctcccacg tggtgagatg atcgagaagc cggaagtcct
    43081 ggaagacgat gccgatccga cgcctgagct gcgcgcggcg tgcaggcgtg atgcggccga
    43141 cgtcctgatc gaagatgtgg agctggccgc gtgtcggcgt aagcgacatg aacaacagcc
    43201 tgagaagcga gctcttaccg gcgcccgagg gacccgtgag aaagtggaac gagccaggct
    43261 tcaggtgaaa cgtcacctcg cgcaggactt ccggcccccg cccgtatttc agcccgacat
    43321 tctccaaccg gatcacgcct cgaccacccc ccgtgattct ccgtatggcc actttatgcg
    43381 aaatgcaccg gagctgatca atcgcaggat tgtggcgggc tcgtgtgctt ggctggcctg
    43441 aggatgagcg ggccggcgga agggcaagcg ccggcagcgg cctgagcggg ccgcatctcg
    43501 tgtctcggag cccttgcggc aacgcaggct gccatgccgc aggaatggcc ttttccttcg
    43561 gttgcggcgg tgtgctaatc agccgatatg taaggccaat ccgagcatga gcagccccga
    43621 ggaacttgta tgagcgcagg caacggcgaa aacgatcgca tcaaagtcgt cttctcgccg
    43681 gaagagatcg cgaggcgaat cgatgcgctg gcgaagcgga tcgcggagca gaacctggag
    43741 cggctgctgg tggtcgcaat cctcaaaggc agcttcgtgt tcgcggcgga tttgatccgc
    43801 gccctgcacc gggtcggcct ggagccagag gtcgacttca tgtcgctgtc cagctaccgc
    43861 aagagcacga gatcgtccgg aaaggtggaa atcctgcggg acgtcgaaat ggacgtcacc
    43921 ggccgcaacg tgctgatcgt cgacgatatt ctcgagtcgg gccggacgct ggcgttcgcc
    43981 aaggacctca tcacggcgcg cggcgcccgc aaggtgctga cctgcgtgtt gttggacaag
    44041 ccggtccgcc gggccgtcca ggtgagcgcg gacctcgcgg cattcgaatg tccgaacgtc
    44101 ttcgtggtag gctacggaat ggatctcgcg catcgctacc gcgagctgcc attcgtggga
    44161 cagctgtcga cggtctgacc gcaggccctg ctcccccacc tcgcagggag ggaacatggc
    44221 gcgcattctt ctggtcgacg acgatcccgc aaccttggac ttcattggcc gggctctctc
    44281 ggctgatggg catgaggtgc tgagctgcgc tgatgcgcag gaggcgctac agaagctctc
    44341 cgctggtcct gtcgatctga tgctgacgga cctcgggatg ccgggcctgg acggtctggg
    44401 gctggccagg cgcgcagcga aggcgctccc cagcatccgc atattactga tgtcgggctt
    44461 cgcgagcccc gacctgcccg cagcgcaggc cctgccgatc gctgggttca tcagcaagcc
    44521 gctgtcgctc gagcaggttc gctctgccgt ccgttcggcg ctcgtctaag ctatccgcgc
    44581 gaagcgcgaa cgcaggctca accgctcacg gtggagggtg tagagccccg cccccatgac
    44641 gatggcgatc ccgatgaagg tgatgttgtc gggcagctcg gcgaagacga aataacccga
    44701 gatgacggcg agcggcgtcg ccacgtaccg gaacggcgag accacggcca tatccccggc
    44761 gcgcattgcc atcaccgtaa agaaggtgcc ggcggaaacg gtgactcccg caatgatgag
    44821 cagaatgagg aggtcggcgg tcggcatccg ccacgattcg aacggcatca gaacgaggcc
    44881 acccagcgtc acgctgaccg ccgagtaaga cgtgagcagc agtgccggca ggctcaagcc
    44941 gatgtgccgc gtcagcagat cgcgcattgc cacgcacagc acggatccca tcacgaacag
    45001 cgctgcccaa tcgaatgcgg cgccgcccgg ccggatgatg atgagcacgc cggtgaggcc
    45061 cgcaaccagc gccatccatc tgcgccatcc gacgggctcg gccaggaaca gcgccgcacc
    45121 ggccgtgatc gccaagggcg cgaactggct gatcgcgctg acctcggcga agggcaggcg
    45181 cagcagcgcc atgaagaaga acagcgttgc gccgacgtcg ccgacggccc tcagcagcat
    45241 gggcggggtg gcgacgaggt aagcgcgccg cagcgcaccg gtgatgtacg cgacacccag
    45301 cgtgagggca ctggtgaaca gaccgcgcac gaacatcatc tcgcccggcg gcagctcctg
    45361 gcccgcgaac ttgatgatgg aatctcccgc cacgaagctc gtcatggcag cgatcatcga
    45421 caggatgccg cgcaggttgt cgctgccggc cgcaaccgat ctcagggacg acagcatggc
    45481 ggtgtgcagg tgacctccgc ggtcccgctc cgtcgtcttt ggagcgggca aaccggccta
    45541 ctctatggct ggcccccgaa accggcaagc gtcggaaaca aaggcagatg aggggtcggc
    45601 gcgcactggg tgcccgggca agaattcctc gacctcagcc cttggcccgc agtggatgct
    45661 tgcggaccgt cacaatggat gccaatgacg gcccagcggt gcctgccgaa ttacggcctc
    45721 tttgcaatga acggtcggcc cattgcgggc ggcggctatt gcccgggttc ggcgtagtgc
    45781 ctaaagtagc attgagatta gtgaataaga acaagacact ctcaaggcat ggatcgactg
    45841 tttgctgcgg gccggcgctg aatgatactg gaatgtcgtg atctcgagcg ctcgtttggc
    45901 ggcctgaagg cgttgcaggg agtaacgctc agcatcgaca agggcgagat cttcggtctc
    45961 gttggcccga acggctcagg caagacgacc atcgtcaact ccatcacagg cttctatccg
    46021 cccgatcgcg gcaacatcct gttcgagggg aaggacatca cgggcatccg cccgcacaat
    46081 gtggcgcggc tcggtattgc gcgcacgttc cagaacctcg cgctcttccg cggcatgagc
    46141 gtgctcgaca acgtcatgat gggccgtcac atccatatgc gcccatcggc gttggcatcg
    46201 ctcttctact gggtatgggc ggagcgcgac gagatccgcc agcgcgagct cgtcgaggat
    46261 acgatcgact tcctgcagct tcaggacgtt cgcgatctgc ccatcgaagt tgtcccgctc
    46321 ggattgcaga agcgcatcga gctggcacgc gcgctggtct ccgagccacg tcttctcatt
    46381 ctggatgagc ccatggcggg catgaaccaa gaggagaagg agtacatggc ccgcttcatc
    46441 ctggatgcac gcgaggagag gggcctgaca gttctgctca tcgagcatca catggacgtg
    46501 gtctcggcgt tgtgcgaccg catgacggtg ctgagctacg gcgagcagat cgcatccggc
    46561 acgcccgctg aagctctttc aaatcccgct gtggtcgaag cctatctcgg aacgaagaga
    46621 cgccatgcgg cctgagggga gcacggccaa agccgtcgaa ctgggtttgg atcttgcctc
    46681 gcgagatccg gacgcggtgc cgcgcgaaaa cgccgggctg gactccggga cgttgatctc
    46741 gttgctcgcg aaaaacgccg agacgcacgg ctcgagcatc gccgtacgcg agcgagatcg
    46801 cggtatctgg caagagttca catggagcga cgttctctcc gaggttctgg ctttcgcggc
    46861 agcccttgag gctcgcggtt tccgggcggg cgacggcatt ctggtgatcg gcgacaatcg
    46921 cccacggctc tatttcggca tgctgagcgc cgcggccctg cgcggctacc cggcaccagt
    46981 ctacccggat gtaccgcctg acgatctcga atactacgcc ggcagcagcc gtgcgcgctt
    47041 tgcgctcgct gaagatcaag agcaggtcga caagctgctc gagttgcgca accgggccgg
    47101 gctgcccgag ttcattgtct acgacgaccc gcgcgggctg tggaactaca ccgagcccgg
    47161 gctgctctcc tgggaggacc tcgtccgcga gggcgctgag cgcctcgccc gcgagcccgg
    47221 gctcaaagag gatctcatca agcgagccaa gccgacggac gccgcggtgt tgctccattc
    47281 ctccggcacg acggggaagc caaaaggcgt cgtgctgcag catcggcgcg tgatcgccgc
    47341 cgcgcgcaat gcggagaagg cggattactt ccgggccggt gatgagctga tggcctatct
    47401 cccgatggcc tgggt
//
LOCUS       DASVDU010000179        45802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1475748, whole genome shotgun sequence.
ACCESSION   DASVDU010000179 DASVDU010000000
VERSION     DASVDU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 45802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45802
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475748"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF226_11395"
     CDS             complement(<1..311)
                     /locus_tag="VF226_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7074637.1"
                     /translation="MSSAVARERPQSSTGGPRHIQHDLLLAALPHPILTLAEAERIIY
                     ANPAAEQFFSAGQATLKRHTLADLVAFGCPLVSLVEKVRRSGLSVNEYGVEVVIPRLD
                     GP"
     gene            complement(308..1315)
                     /gene="dusB"
                     /locus_tag="VF226_11400"
     CDS             complement(308..1315)
                     /gene="dusB"
                     /locus_tag="VF226_11400"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX7074638.1"
                     /translation="MQLLQGPLAIGSRQLINRVVLAPMSGVTDLPFRRLAHRLGAGLV
                     VSEMVASEELARQRRDVLRRAVGEGISPFVIQLAGREARWMAEGARIAEDLGADIIDI
                     NMGCPSRQVTGKLSGSALMRDPVHALSLIEAVVKSVTVPVTLKMRMGWDHNDLNAPEL
                     ARMAEKAGVQLITVHGRTRCQFFKGQADWAFVRRVKDAVAIPVIVNGDISSVAEARRA
                     LEASGADGVMIGRGAYGAPWLPGRIAAALKVGSDPGAPARAEQGGIAAEHVDAMLSHY
                     GRALGLRNARKHIGWYLEQSGAALDVVKAWRARLCQDDDAERVLRGLAAFYDQAEELA
                     A"
     gene            1530..2720
                     /locus_tag="VF226_11405"
     CDS             1530..2720
                     /locus_tag="VF226_11405"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HEX7074639.1"
                     /translation="MQVSVLIVAAGRGTRAAGSQVPKQYRQLRNKPVLTRAIEAFAKH
                     PAISAIQVVIHPDDRALYAACCAGIAADLQEPVDGGPTRQDSVRAGLTALAGAAPDLV
                     LIHDAARPLIEAGLIDRVLAALREHDGAIAALPVNDTLKRSGADGSIAETVSREGLWR
                     AQTPQGFRYKPILEAHLAAHTQGRSDFTDDAAIAEWAGLKVALVPGSAQNIKLTDEED
                     FALAERLIAPSEQMECRTGTGFDVHRFSEGDHVWLCGVRIAHDKGLTGHSDADVGLHA
                     LTDAILGAIGDGDIGQHFPPSDPKWAGAASHIFLRDAARRVTERGGRIANVDVTLLCE
                     APKISPHREAMRNMIAEILEIAVERVGVKATTTEGLGFTGRREGIAAMASATVLLPPC
                     NPSS"
     gene            2819..3298
                     /locus_tag="VF226_11410"
     CDS             2819..3298
                     /locus_tag="VF226_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7074640.1"
                     /translation="MNIMTQEPDSAAAELANIAETTSSSCIATRVRQLSRIITRVYDD
                     AMRPLGITASQFTLLTQLAQQDGITAVEIGFTLDIEKSTLSRNLKRLLALNLILMDPP
                     AGRRGRGLHLTEKGRRVIKEAYPVWLAAQQRAVSVLGPESRATLDTLLHQAEKLAVA"
     gene            3492..3989
                     /locus_tag="VF226_11415"
     CDS             3492..3989
                     /locus_tag="VF226_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEX7074641.1"
                     /translation="MFPADLVDEARQLIGLLRDHKLMLVTAESCTGGLISGVLTEIPG
                     SSDVVERGYVTYSNAAKVSCLGVDPQLIATHGAVSEEVARAMASGALAHSQADIAVSV
                     TGVAGPGGGSVEKPVGLVHFGAVRKGQPVLHLEKRFGDLGRSAIRIESVRTALDLVKA
                     AVRLE"
     gene            complement(3976..4449)
                     /locus_tag="VF226_11420"
     CDS             complement(3976..4449)
                     /locus_tag="VF226_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEX7074642.1"
                     /translation="MPSFRTQRIVKHSPREMFELVSDVERYPEFLPLCEKLVLHSRGL
                     KQDGTEEILATMTVGYGPINESFTTRVRLDRVKQEIFVSYVNGPFRHLENTWGFRPHP
                     DGCRVEFSIAYEFRSMALGMMMGALFDRAFRKFAEAFEARADEIYGDEGPALTRA"
     gene            complement(4558..5508)
                     /gene="lipA"
                     /locus_tag="VF226_11425"
     CDS             complement(4558..5508)
                     /gene="lipA"
                     /locus_tag="VF226_11425"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7074643.1"
                     /translation="MVTIVDMRPRHPEKAHKPDTPILRKPEWIRVKAPGSPGYVETKR
                     IVREQGLHTVCEEAGCPNIGECWERKHATMMIMGDTCTRACAFCNVRTGLPRPLDPQE
                     PANVAEAVAKLGLEHVVITSVDRDDLADGGAGHFAAVIRAIRERTPGTTIEVLTPDFL
                     RKDGALEVVVEAKPDVFNHNLETVPGLYLQIRPGARYFHSLRLLQRVKELDPTMFTKS
                     GIMVGLGEKREEVLQLMDDLRTADVDFLTIGQYLQPTRKHAEVKRFVTPEEFKSYERI
                     AYAKGFLLVSASPLTRSSYHAGDDFARLRAARLAAMGQAG"
     gene            complement(5624..5887)
                     /locus_tag="VF226_11430"
     CDS             complement(5624..5887)
                     /locus_tag="VF226_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7074644.1"
                     /translation="MDFGTYSPWLTFIVVGLVAGWITGMLFGQRGLIRYLIVGCLGAL
                     VGAYVIHGLLGFSFGLGSAFLDQVAVATIGAIAVTLLARAIAK"
     gene            complement(5977..6795)
                     /locus_tag="VF226_11435"
     CDS             complement(5977..6795)
                     /locus_tag="VF226_11435"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7074645.1"
                     /translation="MSDVVARHYAGGGDLAKAIADRLRNAGKVIDNLTTADLATVDEF
                     HIRGRQATLELAQSLNLSATSHVLDIGSGLGGPARTLAETYGCRVTGIDLTPEFCEAA
                     TTLSNWVGLGDRVAFRQGDATDLPFGDAEFDAAVTIHVAMNIAAKDKMYAEAKRVLKP
                     GARFGVYDVLQGEGGEVLFPVPWARDPSISHLATPDEMSLLLTGAGMTVLQVVDSTEE
                     SARWFEAMVARMAQSAPAVTFQTFLGNDFPLMARNLVQNLRDRRIRTVAYVCES"
     gene            complement(6884..8329)
                     /gene="lpdA"
                     /locus_tag="VF226_11440"
     CDS             complement(6884..8329)
                     /gene="lpdA"
                     /locus_tag="VF226_11440"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7074646.1"
                     /translation="MPDNSYDVVVIGTGPGGYVAAIRAAQLGFKTAVVEREHLGGICL
                     NWGCIPTKALLRSAEVYRNAEHAGAYGLSIEGKLGFDAGAIVKRSRGVSSQLNSGVGF
                     LMRKNKIDVIWGEATISKPGELVVSETKKKPMQPAISPPKGVLGTGTYQAKHIIVATG
                     ARPRVLPGIEPDGKLIWTYFEAMVPPAMPKSLLVMGSGAIGIEFASFYRTFGVEVTVV
                     ELLPQILPVEDAEIAAFARKQFERQGMRILTGASVTKVEKGKDSITATVDLGNGKSEQ
                     ITAERLISAVGVVGNVENLGLEKLGVKVERGAIVTDGLGRTNVPGIYAIGDVAGPPLL
                     AHKAEHEGVLCVETIKGLPTHPMDKLRIPGCTYCYPQIASVGLTEAKAKEAGYELKVG
                     RFPFRANGKAIALGEPEGMTKTIFDAKTGQLLGAHMVGPEVTELIQGFVTAMQLETTE
                     EDLMHTVYPHPTLSETMHESVLDAFGRVIHT"
     gene            complement(8445..8717)
                     /locus_tag="VF226_11445"
     CDS             complement(8445..8717)
                     /locus_tag="VF226_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074647.1"
                     /translation="MKHGAILAAVVALAGCASNAGPYGDPGARPLPAGYSCQSVRAEL
                     NRLDAKGIRPKVEAASAGKKLSQQTQADVDRYNYLLNVYLGARCHV"
     gene            complement(8913..10193)
                     /locus_tag="VF226_11450"
     CDS             complement(8913..10193)
                     /locus_tag="VF226_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074648.1"
                     /translation="MAAATARRQAAAREKSADAEPRAHPIPSTSGASGQASTVDPRMH
                     APTTNQSAPAAPAAGLDDLTRIRAIDAALQKRLNALGVRRFADIAAWRKEDVRFISQA
                     LGFRGRIERENWIEQAQILMGGNETAFSRRGHVGLVAKPAADQGDEMPATAATAASVS
                     QPTEASGVAEAVAAAVSAAQPRRAAATAGRDNLQRIRRVSEDIEHLLNAQGVTRYEQI
                     AQWTALDVARFEHLMGSDGRISRENWIEQAHLLASGGETAYAREFDLKQAASARPRPT
                     QLAEAIREAQAAKTDGASPPPRPEEGQPRKVDLSTLRSVRSEALVGAKQESAVGIGPG
                     ERLAARPDDLKRIRGIGVLFEKRLNGMGYWTYEQIANWTAADVARVSKALEVKGQIER
                     ENWIEQARMLASGQVTQFARRRDRGEVVVNRTTD"
     gene            complement(10833..13514)
                     /locus_tag="VF226_11455"
     CDS             complement(10833..13514)
                     /locus_tag="VF226_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7074649.1"
                     /translation="MRCNPLRWLVGSVLVLGLLGVMNMQGVLAQIEAELAQEARQSLA
                     QAGLDWAEVEFSGRDSKLLGIAPDENERRKAYQITSSLWGVRKVSNRTDLIAEEKNYV
                     WRAQLRDDRLKLTGFVPNEQTRRAIIGAAKATFPQLEVQDRMKLARGAPDTEIWLGAV
                     SFSLKQLSRLKPGAAVQLESDGLEIVGEAEDSAAFRSIKSALATGLPQGIALKQDRVK
                     PPVVDPYIWAARLSGTQLQLGGYVPSEDAREEVFAAAQEAFPKAVIVDRMRIAAGEPD
                     GLVSAAVGALGKLAQLEQGDIEIKGKELLISGLATKEETANFLRETLREGIPKSIKVD
                     EKIKFREPAIKPVSPYTIGAAIDNDKLLLTGYVPSEAARAELVKAAKSYFPKLDISDG
                     LKLGANAPDGWQTCMTSGMASLVKLGNGRIEMVDNKLIFAGVTEDEQVAEAVRNELRA
                     AVNRACELDARLVVDTPPEPDLSWRASFAEKQVVLEGDVPDEKTKEELQQAAAKLFPE
                     AHIVSRMEIKAARSEKWAKVSDTGLRLLARLRGGEVRILGNELSIVGQAPDAAVVTLI
                     QQQLKDLPSGYRGIDSIEVRSDAMIWAEQEAKRKAEAEARRKAEEDAKLKAEEEARKA
                     EEERLAAEAERLRQEENLRKAEEQARAAAAAEEARLAAEAERLRQEESLRKAEEQARA
                     AAAAEEAAAAAEAQKKAEAEARAHAEEEARRRAEADAAADQHTPGSDDTDAKQASAAK
                     PESEEVALAVQQPPSDAAAEQHAAVAPEHLEADRRCQRAIANATLHGTVNFARASAKL
                     NATGKETLSRLLEVARSCPNVRIHIEGHTDAEGSPEGNQVLSEERAKAAVDYLVSRGV
                     PIEKISAVGYGATRPVAANDTPESRAMNRRVEILIIE"
     gene            14026..15522
                     /locus_tag="VF226_11460"
     CDS             14026..15522
                     /locus_tag="VF226_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7074650.1"
                     /translation="MKQYQTYIDGKWCDAASGKTFQSSDPYTGEPWALIPECDAIDVD
                     RACNAAHRAFESGPWPAMTQTARGRLLRKIAGLIEKHGEHLAKIEVKDNGKLISEMSA
                     QLKYLPEWYYYYAGLADKITGMVIPVDKPDHFTYTLREPVGVCGLIIPWNSPLLLVGF
                     KLAPALAAGCTVVIKPSEYTSASLLEFVKLVIEEADVPPGVVNVVTGYGATAGEALVT
                     HPKVAKVAFTGGSATGSRVASLAAASFKKVSLELGGKSPNIVFDDCIMDEAVNGAISG
                     IFAATGQTCVAGSRLLVQESIHDAFVEKLVATAKTAKLGNPMSFETQVGPVTTPPQYQ
                     KVLSYIDIAKSEGANCVLGGGPAGPEMGGGKYFVQPTIFTGVDNKMRIAQEEVFGPVL
                     SIIKFKDEEDAIRIANDVIYGLAAGVWTQSVKRATIMPRRIRAGTVWVNTYRAMSFTM
                     PFGGFKSSGLGRENGQTAIDEYLETKSVWVSTASNTGNPFVMKTSAPS"
     gene            complement(15664..16194)
                     /locus_tag="VF226_11465"
     CDS             complement(15664..16194)
                     /locus_tag="VF226_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074651.1"
                     /translation="MLRKKFSSGPRAGAVAAATPLASRLNEPERLVGLGFRSWLAGYQ
                     TGDISCWEEAWSIFSNALGPTQARSAVTDLACWVRAIRNCSCRQIEIYPGQCAGFCRD
                     ECMAIAMVAASQHGACPAMRACAWALLQTSAIDDVVEGAECLGARLKSCDKILSPASI
                     CEAVAHCPARASGRPH"
     gene            complement(16277..17332)
                     /locus_tag="VF226_11470"
     CDS             complement(16277..17332)
                     /locus_tag="VF226_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7074652.1"
                     /translation="MTYSTKGRRALRAFAHASFSLAAIALFPLAAHADGEVNIYSYRE
                     PGLIDPLMKAFTEETGIKTNVVHSRSGLNERLAAEGRNSPADLLFTVDIGRLNEAKTM
                     GVTQPVKSEALEKAIPAAYRDPEGHWFGLTLRSRVVYASKERVSQDSITYEELADPKW
                     KGRICSRSGQHPYNISLIASMIAHHGEAEAEKWLRGLKENLAQKPSGGDREQARDVYS
                     GKCDLAIGNTYYVALMMKNPEQKAWADAIKVLFPNAADRGSHVNISGMALAKYAPNKE
                     NAIKLMEFLASPKAQEIYAQANNEYPISPEAKPSKIVQSWGALKPDTLPIETIASLRK
                     KASELVDKVDFDAGPSS"
     gene            17649..18629
                     /locus_tag="VF226_11475"
     CDS             17649..18629
                     /locus_tag="VF226_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7074653.1"
                     /translation="MTKDSPVTPASDDWRPAARYPDPAITALDPRFENYWLKLASVER
                     IATGCRWAEGPVWFGDGRYLLWSDIPNNRILKWEEETGAVSIYRKPSNYANGNTRDRQ
                     GRLITCEHGGRRITRTEYDGSITVLMDSFEGKRINSPNDVVVKSDGSIWFTDPVFGIL
                     GHYEGHKATPELEANVYRLDPETGEATIVAEGVLGPNGLCFSPDESILYIVESRGIPN
                     RKILAYDVIDGGRKITNKRVLIDAGPGGTPDGMRCDVDGNLWCGWGMGTPELDGVMVF
                     APDGTPIGRIALPERCANLCFGGLKRNRLFMAASHSVYALYVNTQGAVGG"
     gene            complement(18638..19402)
                     /locus_tag="VF226_11480"
     CDS             complement(18638..19402)
                     /locus_tag="VF226_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074654.1"
                     /translation="MMGRVLVVLPLVAAYVISLAILFRFFTQSRSSHVLVLECKARKE
                     SEPENCNRNFLSNAFPIALAVGLLFAEAGALVLLAEAFNSIPADLSANIHLMLHYEPM
                     LPGRSAALAILICSSSVQLLARRFIRASVAYPVLLLLTGIISFGAAVDIIFKGLSPAP
                     AAVIFQVAGCLQIMAAAAFGLGALILGAETLAGLMHAALAQAAGAGVRLLGVIAMLSI
                     WSYLPPASAVALLLAGVLVPGAATALTSIAALATTP"
     gene            complement(19410..20660)
                     /locus_tag="VF226_11485"
     CDS             complement(19410..20660)
                     /locus_tag="VF226_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7074655.1"
                     /translation="MLLLEAFILLTALLGLALAVRQLLLGWCVWRFSPSAGDHPSRIP
                     ENWDWPSVTVLIPAHNEERVIAGSLAAMTALDYPSRKLSILVVDDRSEDRTGRIADSF
                     VEKDPRVAVLHRDATAKPGKAAAVADGMAKSSSDIIVLFDADYLPPPGLLKELVRPFE
                     DPDVGATMGRVVPLNADRNLLTKLLDLERRAGYGTDQNGRALLDLVPQFGGTVGGIRR
                     AALDSVGGWNVGHLAEDTDLTFRLVLGGWRVAYVCQARCYEEVPEDWGSRFRQVRRWA
                     YGHNQCLLDHFGAILRSERLRLGQKLDALLILTFYAFPVLALVSMFAAVPLLAMGGKS
                     TPDGIGWVIASLFGIALLAPHAQVIVAAHSDGQRHVLRVLPLLFVSSCLSLLASTAGA
                     ALLIRDRLFGLSFGWDKTRRYRSA"
     gene            complement(20679..21479)
                     /locus_tag="VF226_11490"
     CDS             complement(20679..21479)
                     /locus_tag="VF226_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEX7074656.1"
                     /translation="MPAIVAGLLFASGLLLSTSAMAQGMQVTRSDITTVFGYYGVSHG
                     ISDRRFTTVWGNTLFSNNLGVHAEAHYMDREETAGFFAGGLSWVGDSAEVRGWLGTSS
                     ENDSILPELYGRVEGTFRTSAETGLVFHSALAYRSFRNSAEETAAEIEIAKYIPLETG
                     NLILSALARVVHASPGDHLSSAFGAGLAYVHPRNVSVGVHVEGGRATYDGVLAPGQFD
                     EQYVSIRPVVSFYLTDDIELVGLMEYSSRESYDVYGGHVGLKVHFDQP"
     gene            complement(21594..22850)
                     /gene="wecB"
                     /locus_tag="VF226_11495"
     CDS             complement(21594..22850)
                     /gene="wecB"
                     /locus_tag="VF226_11495"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7074657.1"
                     /translation="MRVLSIIGTRPEAIKMAPVIKMLQNNAKAGVESLTVFTGQHEQI
                     VYPIFGFFGIAPDFDLGVMKKAGSLNQLFSALMGELERIFSETQPNMVLVQGDTTSAA
                     AAAIAAFHRKIRVGHVEAGLRTGNLDEPWPEEWYRRLITLSSGLHFAPTTVAKRNLIA
                     EGIPPDRIIVSGNTVVDALRSATGRLRTDGRLKARLESQFAFLDGARDVVLVTAHRRE
                     NMGEGIEKICRAVLRLCERRDVAIVFPVHPNPQVRVPVMERLGGRPNIHLIGPADYGA
                     FVYLMMRSSLILTDSGGIQEEAPSLGKPVLIMRDVTERREAVEAGPARLVGTDPDSII
                     AAVSAILDGKETGLTSRHSANPFGDGRAAERIVQAVLSEGLSNFCSDLEVEAAKRPRP
                     YRAARIMHRMPRQAWLKRWQSVTASA"
     gene            complement(23188..23634)
                     /locus_tag="VF226_11500"
     CDS             complement(23188..23634)
                     /locus_tag="VF226_11500"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7074658.1"
                     /translation="MSSSQRRPPAHRSIFKHFRIIPTRWMDNDLYGHVNNVVYYSYFD
                     TLVNRYLIDPGGLDIHTGQTIGIVVETSCRFHRSFAYPEDIEAGMRVAHLGTSSVRYE
                     IGLFGLGEPEARADGYFVHVFVDRQTRRPVPISGRMRSALAEIAGD"
     gene            complement(23693..24040)
                     /locus_tag="VF226_11505"
     CDS             complement(23693..24040)
                     /locus_tag="VF226_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7074659.1"
                     /translation="MTITRHESTAILSNVVEANGFVFTAGIVADDRNLDAKGQTEQIL
                     NQIDRLLGLCGTDKSKIVSATIWVTDIRNREPMNEAWKAWTGGNDLPARACIEAKLAD
                     PKLLVEIAVIAAK"
     gene            complement(24165..24959)
                     /locus_tag="VF226_11510"
     CDS             complement(24165..24959)
                     /locus_tag="VF226_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX7074660.1"
                     /translation="MADAPVPAQTFARDLAKGSTEETAAQIAHGINAPWLRRRSGNRQ
                     QVNGTRPGPGEGGGKPDMPEFRDTARTVLPVDYQGREIMFEAYAYEDDSASYQALALV
                     YRSPAANGSDELPLVRVHSGCVTGDIFHSLRCDCYLQLQKAIGRIIESKTGAIIYLPH
                     HEGRGIGLVAKIKAYAAQDRGLDTVDANLSIGLPVDARDYQLPAKILKDLRFTDIRLL
                     TNNPLKVEALQKHGINVVEQIQHATSPSPYNARYLATKRERMAHKL"
     gene            complement(25422..26393)
                     /gene="denD"
                     /locus_tag="VF226_11515"
     CDS             complement(25422..26393)
                     /gene="denD"
                     /locus_tag="VF226_11515"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX7074661.1"
                     /translation="MKVLIIGGAGMVGQKLAQRLAKDGKLGDKPIDSLTLYDVVEARC
                     PDCPFPMSVKVGDLSQPGQAEALVADKPDVIFHLAAIVSGEAELEFDKGYRINLDGTR
                     NLFEAIRHAGNKPRVVFTSSIAVFGAPFPEAIGDEFFHTPLTSYGTQKAIGELLLADY
                     TRKGFFDGIGIRLPTICVRPGKPNKAASGFFSNIIREPLAGQEAVLPVSEDVRHWHAS
                     PRAAVGFLLHAATISGEQVGPRRNLTMPGLSVTVGEQIEALRKVAGDKVVARIRREPD
                     PMIMQIVAGWPRNFDARRASELGFKADASFEEIIRIHIEDELGGKIA"
     gene            complement(26414..27190)
                     /gene="otnI"
                     /locus_tag="VF226_11520"
     CDS             complement(26414..27190)
                     /gene="otnI"
                     /locus_tag="VF226_11520"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX7074662.1"
                     /translation="MPRFAANLSTMFNELAFLDRFEAAAKAGFTGVEFLFPYEHPADE
                     IAARLDANGLTQVLFNLPPGDWTKGERGLAALPGREEAFAAAMRQALTYAEALKCPRV
                     HAMAGLVHHGASRATYVANLKKAARMAQDFGVEVLIEPINTRDIPGYFLNRTGEARAI
                     IYEVGEPNLGLQFDIYHRQVMEGDIATAIREYAPLTSHYQIANPPDRGEPDEGELNYG
                     WLFRLIDETGYEGWIGCEYKPRRGTVEGLKWAERCGVRLG"
     gene            complement(27361..28113)
                     /locus_tag="VF226_11525"
     CDS             complement(27361..28113)
                     /locus_tag="VF226_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074663.1"
                     /translation="MANRLQGKVAFCTASGAGIGRATAIAFAREGARVIATDIDGSKL
                     EGLEKEGIAECLELDVRDTGSVEVLAKRFSPVDILFNAAGFVHHGTVLDCTDAEWDFA
                     FDLNVRSMHRTIKAFLPGMLENGGGSIINIASGAGSIRGVPNRYVYGATKAAVIGLTK
                     SVAVDFIRRGIRCNAICPGTIQSPSLEERIAALAKTVGSVEKARQMFIDRQPMGRLGT
                     AEEVAAMAVYLASDESAFTTGTTMLVDGGFAL"
     gene            complement(28235..29137)
                     /locus_tag="VF226_11530"
     CDS             complement(28235..29137)
                     /locus_tag="VF226_11530"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7074664.1"
                     /translation="MADRPRSKRAGTSVVELARAKINLTLRVLGRRPDGYHDLESLVV
                     FADVGDLVTLEVGLEPAVTMTGPFASKVAGENLAAKALDVLAAAEPDLRLGAVRIEKN
                     LPVASGLGGGSADAAATLRAVRRANPGFAGTIDWLGLAARLGADVPVCIAQAPALMWG
                     VGEKLRLLHDLPQLPGVLVTADDPVPADKTREVFRRLDAPALETTGRAPEPPPAFGTP
                     AEFITYLQAFSNDLTEPAQDLMPVCKAVEKELAAYPGCRLVRMSGAGPTVFGLFESVE
                     AARSAADALAEGHRGWWVRSVMLG"
     gene            complement(29173..30987)
                     /locus_tag="VF226_11535"
     CDS             complement(29173..30987)
                     /locus_tag="VF226_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7074665.1"
                     /translation="MRSRRLFLWMACLGTVAATAVATATAQRLSAVTAKPDLKVEVES
                     LLGSYLAGRYAREQRDTSAAAEFYSRALARDPDNEVLIEQTFLMEATEGNWPRAIELA
                     QAIAERHDSHRMAQLVLGLSAFKSGNFAGAEEHFRAAGSGPIGELTSTLARAWVKLAI
                     GDVDGALGLLEAGKQAEWAQFYIRYHRGLIADIGRRPAEAGKAFALVFKMDARTPRTA
                     MAYAQHAAKAGDLKLAKRILHQHLSKTPGEGHPTSRDLLDRLERGEKVPFLVSTVEEG
                     LAEVFYGLGEALTGEGGISIGALYLQMALYLKPDFPFAMAALANVYESTKRYADAIAI
                     YDKIPTGTALDTSVAIRKAINLNALDRTDEAKALLDKLIEKNPKDLRVLDAIGTIMRG
                     RKRYAEAVNYYTRAIKLIKKPEERHWTYWYARGTSYERLKKWPEAEADLKKAMRLSPD
                     QPLVLNYLGYSWIDQNRNLREGLKLIEKAVALKPDDGYIVDSLGWAHYRLGNYDEAVR
                     FLERAVELRPEDPVLNDHLGDALWRVGRVREARFQWQHALTLNPEPEELEKIKGKLED
                     GLTDEPVAGSPKKSRQAAREDAGKRRAQTKLAPGAPIQ"
     gene            complement(31156..32124)
                     /locus_tag="VF226_11540"
     CDS             complement(31156..32124)
                     /locus_tag="VF226_11540"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEX7074666.1"
                     /translation="MFTLSELESTLPLVREMVPPTPQYAWPLLARRAGCELWVKHENH
                     TPTGAFKIRGGLVYIHELKAARPDVTGVISATRGNHGQSIALAARRAGLDAVIVVPHG
                     NSVEKNAAMEAFGAELIVHGRDFDEARVYAAEIAEQRGLHFVPSFHPHLVRGVATYAL
                     ELFQAVGDLDVVYVPVGMGSGICGVMSVRDLLGLGTEVVGVVAEQAPAVKMSIDAGHL
                     VSTPSARTFADGMACRDPHPDAVELIRAGAGRVVQLGEEEIAEAIRIYFSETHNVAEG
                     AGAAALAAVLSESDRLRGKKAAVILSGGNIDRDVFLEVLNGRTPSA"
     gene            32379..33479
                     /locus_tag="VF226_11545"
     CDS             32379..33479
                     /locus_tag="VF226_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEX7074667.1"
                     /translation="MRSANAIDFWRGVALLMIFVNHIPGNAFGQLTLRNYAISDAAEL
                     FVFLAGWSLSYATGGPHHPEPYARTFFRLISRAIELYRAQLVITMLALALLASVAIIR
                     NNPLFLEWHNAASAFYDPIRATIGIVLLTHQLGYFNILPLYIVLLLFAPLIVVLTRWR
                     VWSALALSLAVYGFALATGVSLPNWPAEERWFFNPLSWQVLLVLGFVGAELSRSSERF
                     NQIVARLVPLGWLLAAVGAYLALNQLWPDPMRVPEPKLLFLFDKTYLSPARIISLLAI
                     AVAFQGAFKYIERPLGPITSLMCAYGRNSLAVFCVGSLLSLIGQVVRAILPESFLLDC
                     ATVLCGVCLMGFTAWFVEWRSRIPGLVSPRAS"
     gene            33422..34252
                     /locus_tag="VF226_11550"
     CDS             33422..34252
                     /locus_tag="VF226_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7074668.1"
                     /translation="MVCRVEKPHTWLGVAASFLICASAAAQEQVSPQQRFQVATLSRA
                     ASELSSDCSVKKAYGPRAPLYSVRRALREQRPVKVLAIGSSSTVGVGASSPAAAYPAR
                     LEVTLEGFLPGVDIRMMNRGVSGETGRGAVERLKLEVAETMPDLVVWQVGTNDALARI
                     DEDDFAETLREAIDWLEANRIDIVLIDPQYVERLAGDGYYTRIVKTIAEVAGKERVLL
                     VNRFEAMADLAKRKGNSAFLAQDRFHLNDVGYRCMAEYAARAIAAGILQAEAEAARQN
                     "
     gene            34412..34894
                     /locus_tag="VF226_11555"
     CDS             34412..34894
                     /locus_tag="VF226_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074669.1"
                     /translation="MIHPVLGGLLAVLGALSIATPASATELAQTSANVAGSAPEFSQA
                     AADLEKGQMFMTRGLVCDQPSEIDAVITLARKGEPLEGALQQINTGTETPRCVVGKML
                     IAQYMKQARTFLVNQQMFHVHQVRVIGVAVPTPRGVVPVKLKKPMEQYVVSADDRKAA
                     "
     gene            complement(34920..35402)
                     /locus_tag="VF226_11560"
     CDS             complement(34920..35402)
                     /locus_tag="VF226_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX7074670.1"
                     /translation="MSSDILRSHLPETTMVARMLLAIVLGAAIGFERELRSRPAGLRT
                     HILVSMAAASFTLITIEIFYEMGNRGGTQVSPADPARIVDAVTAGVAFIAAGAIIKSQ
                     GDVKGVTTGAAIWLAGAIGVACGIGYYLLAALATGLAVLVLQGIGLLERWWLGKETDT
                     "
     gene            complement(35489..36391)
                     /locus_tag="VF226_11565"
     CDS             complement(35489..36391)
                     /locus_tag="VF226_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7074671.1"
                     /translation="MTIAVQALTSRPLVGILFMCAAHMLFPVMNGLVQVLSDRYSSEQ
                     IVWARTASHLIFVLALFTPRHGLRIVVSNRPGLQLMRSLLVLATTMLFFSGLVYVPLA
                     KAASISFAGPFLIVLLAWPMLGERISGHRLAAVVAAFIGVLVVVRPGTEVFHWGSLLI
                     LASAVTYAFDQVFTRRVAEYDRAETSAIYSVLIGTVVMSVITAFAWTTPPTWTDVGLL
                     AMLGVIGGLGHWCVAKSMTYAPANLVAPFMYVQMIGAVIVGYLLSGTLPDLGTWVGSA
                     IIIAAGLFIGLREARGANLRSRAG"
     gene            36604..37548
                     /locus_tag="VF226_11570"
     CDS             36604..37548
                     /locus_tag="VF226_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7074672.1"
                     /translation="MQRARNWERMEKSDAPGSEQQRTDRLKAIALTLLAVTMFACLDG
                     TAKYLVSVELLPVGQIVWMRFFGHLLLVAVVLGFVSIPRLLRTHKLKYQLARSLLMLS
                     ATLLNVIALRYLRLDQTTTILFLAPLTVALLAGPTLGEWVGWRRLVAILVGFMGILIA
                     IRPGISAVHPAFLLCLGGMLSYSLYILMTRYLAAHDTSEVTLFYSTVVGTIFAAPWAL
                     SEWVWPTGPLGWILLLSLGFWGGASHYVFIVAHRWAPTSALAPFLYGQLLAATAIGYF
                     VFDQLPDVWTITGAGIIVASGIYMLHRERIVKREDVED"
     gene            complement(37646..38251)
                     /gene="gmk"
                     /locus_tag="VF226_11575"
     CDS             complement(37646..38251)
                     /gene="gmk"
                     /locus_tag="VF226_11575"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7074673.1"
                     /translation="MLVLSSPSGAGKTTLARRLLEADSGIEMSVSVTTRKPRPGEVEG
                     RDYYFVSESEFRRMRDAGELLEWARVFDNYYGTPRSPVERALAGGRDVLFDIDWQGAQ
                     QLNEKAAQDLVRVFILPPSGEVLEQRLRSRAQDAPDSVARRMADAAAEISHWAEYDYV
                     IVNADLEASVAGLRAILAAERLRRERLLGLSDFVREMQKSL"
     gene            complement(38290..39177)
                     /locus_tag="VF226_11580"
     CDS             complement(38290..39177)
                     /locus_tag="VF226_11580"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7074674.1"
                     /translation="MTLKSMTGFGRADGAAGPMRWHWEVRSVNSRGLDIRLKLPAGFE
                     ALEPRVREAVAKRLKRGNVAIALAVKREEAVSQIRLNHDLLQQVLTAAERVRVAVGGG
                     QVSVDSVLGVKGVLEVVEASETDEEAEARALVMLASLEEALQAVENARASEGARLREV
                     LLDLTLSIERAVEEIAASPARTPEAIRQRLREQVERLLDASSAFDEARLHQEAVLLAT
                     KADIEEELQRLRAHVAAARELLDTGEPVGRRLDFLSQEFNREANTLCSKANDGQITRL
                     GLHLKALIEQMREQIQNIE"
     gene            complement(39375..40550)
                     /gene="mltG"
                     /locus_tag="VF226_11585"
     CDS             complement(39375..40550)
                     /gene="mltG"
                     /locus_tag="VF226_11585"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7074675.1"
                     /translation="MGPTLRFINAFLTFSFLALAVAAGGVFWVNSELDKAGPLQEARP
                     LVIRRGEGARDIARRLEREGIISREHVFVAYYLARQMSGWFGGKPLQLKAGNYELKPA
                     MSMREVADLIGEGKVALLRLTIPEGLTSHQIVERIRADQNLTGEISVVPEEGTLLPDT
                     YLVPRGTTRQAVLDMMRAEREKFLQAAWKERQPDLPLQSMDEAVILASIVQRETGRND
                     KFEDIASVFINRLRRKMPLQSDPTILYGLDGGQVDWGRPIYRTEIQRKTSHNTYQING
                     LPPTPICNPSREALRAVLNPSRTNYLYFVASGTGSSVFSETLDEHNRAVAKWRKVEQE
                     IRAREAEAAKEAAAKQLTTAGGPAAASGGPGLVNPAAAAAGTSSEGDPPLPLRKPRQ"
     gene            complement(40884..42149)
                     /gene="fabF"
                     /locus_tag="VF226_11590"
     CDS             complement(40884..42149)
                     /gene="fabF"
                     /locus_tag="VF226_11590"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7074676.1"
                     /translation="MRRVVITGLGLVTPLASGVEATWSRLLAGRSGARRVEEFDVSDL
                     SCQIACFIPRGDGSDGTFNPNDWMEPKEQRKVDDFIIYAVAAADQALADSGFEAKTPE
                     QQERAGVLIGSGIGGLQGIAETSILLKEKGPRRVSPFFIPGRLINLASGHVSIKHQLK
                     GPNHAVVTACSTGAHAIGDAARLIALGDADVMVAGGTESPICRIAIAGFAACRALSTN
                     FNDEPTRASRPYDKDRDGFVMGEGAGAVILEELEHAKARGARIYAEVIGYGLSGDAYH
                     ITAPAEDGDGAYRCMKAAIARAGIDPSDIDYINAHGTSTPMGDEIELAAVERLFGNAA
                     AKLSMSSTKSAIGHLLGAAGAVEAIFGVLAIRDNVVPPTLNLDNPSVETAIDLVPHTA
                     KQKQVDTVLSNSFGFGGTNASLILRRFVG"
     gene            complement(42248..42490)
                     /locus_tag="VF226_11595"
     CDS             complement(42248..42490)
                     /locus_tag="VF226_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7074677.1"
                     /translation="MSDVAERVKKIVVEHLGVEAEKVTENASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAEHILTVGDAVKFLEKNANAA"
     gene            complement(42766..43503)
                     /gene="fabG"
                     /locus_tag="VF226_11600"
     CDS             complement(42766..43503)
                     /gene="fabG"
                     /locus_tag="VF226_11600"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7074678.1"
                     /translation="MFSLAGRGALVTGASGGIGSAIARTLHAAGATVAISGTKIDRLE
                     TLAGELKERVHVVPCDLDDRAAVQKLAGEAEAKLGTLDILVNNAGMTRDNLFMRMKDE
                     EWDQVIAVDLTSAFMLCRAAVRNMMRRQWGRIINITSISGIIGNPGQGNYAAAKAGMI
                     GMTKSLAREVAARGITANCIAPGFIKTAMTDALTEKQVEAIAQHIPAQTFGVPEDIAA
                     AALYLASEEARYMTGQTLHVNGGMAMV"
     gene            complement(43509..44456)
                     /gene="fabD"
                     /locus_tag="VF226_11605"
     CDS             complement(43509..44456)
                     /gene="fabD"
                     /locus_tag="VF226_11605"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7074679.1"
                     /translation="MAVAFVFPGQGSQEVGMGRELAETYKAARDVFDEVDDALGEPLS
                     RTIWEGPKDVLTLTENAQPALMAVSMAVVRVLEREKGVSLADHVSYVAGHSLGEYSAL
                     AAAGALSVTDAARLLRARGKAMQQAVPVGQGAMAALLGVGLDVAEKVAAEAAQGDVCQ
                     VANDNEPTQVVLSGHKSAIDRVAEIGRKHGVRRALPLPVSAPFHCSLMQPAADVMAEE
                     LSKVEVRRPVVPVVANVLAEPISDPDEIRKRLVEQVTGTVRWRECVQKMGALGVTDFY
                     EIGAGKVLAGLVKRILKDAKAMSVGTPADVDAALAALGR"
     gene            44863..45330
                     /gene="rpsF"
                     /locus_tag="VF226_11610"
     CDS             44863..45330
                     /gene="rpsF"
                     /locus_tag="VF226_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX7074680.1"
                     /translation="MPLYEHVFLARQEVSPQQVEAMINEFGQVIKDSGGTVGKTEYWG
                     LKTLAYKIKKSRKAHYALMNIDASPEAVAEMERRMSLSSDVLRFLTLRVEEHETEPSV
                     QMRKGEDRRDERGARGDRGGGRGGARTEGASTFRARPAGDGSRSRSEQSGSEE"
     gene            45334..45585
                     /gene="rpsR"
                     /locus_tag="VF226_11615"
     CDS             45334..45585
                     /gene="rpsR"
                     /locus_tag="VF226_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX7074681.1"
                     /translation="MADVSVAGGPRRPFHRRRKTCPFSGQNAPKIDYKDVRLLGRYIS
                     ERGKIVPSRITAVSAKKQRELARAIKRARFLGLLPYVLK"
BASE COUNT         8218 a        15003 c        14176 g         8405 t
ORIGIN      
        1 ggcccgtcga gccgcgggat caccacctcg accccatatt cgttgaccga caggcccgag
       61 cggcggacct tctcgaccag gctcaccagc gggcagccga atgccacgag atccgccagc
      121 gtgtgccgct tgagcgttgc ctggccggcc gagaagaact gctccgcggc cggattggcg
      181 tagatgatcc gctctgcctc cgcgagcgtc aggatgggat gcggcagcgc ggcgagcagc
      241 aggtcgtgct ggatatgccg cgggccgccg gtgctcgact gggggcgctc gcgcgcaaca
      301 gcgctgctca tgccgcaagc tcctctgcct gatcgtaaaa cgccgcgagg ccgcgcagga
      361 cgcgctcggc atcgtcgtct tggcaaaggc gcgcccgcca cgccttcaca acatccaggg
      421 cagccccgct ctgctccaga taccaaccga tgtgcttgcg ggcattgcgg aggccgagcg
      481 cgcgcccgta atgggacagc atggcgtcga cgtgctccgc cgcgatgccg ccctgctcgg
      541 cgcgtgcggg agcgccggga tcgctcccca ccttcagcgc tgccgcaatc cgccccggca
      601 gccatggcgc cccgtaggca ccacgcccga tcatgacgcc atcggcgccc gaggcctcca
      661 gagcacgccg ggcctcggca acagacgaaa tatcgccgtt gacgatcacc gggatggcga
      721 ccgcgtcctt cacccggcgc acgaacgccc aatcggcctg ccccttgaag aactggcagc
      781 gagtccgccc gtgaactgtg atgagttgca cacccgcctt ttcagccatg cgggccagct
      841 ccggcgcgtt gaggtcgtta tggtcccaac ccatgcgcat tttgagcgtg accggaaccg
      901 tcacgctctt caccaccgcc tcgatgagcg acagagcgtg cacgggatcg cgcatcaggg
      961 ccgagcccga gagcttgccc gtgacctggc gggaggggca gcccatgttg atgtcgatga
     1021 tgtccgcccc gaggtcttcg gcgattctgg cgccctcggc catccagcgc gcttcgcgac
     1081 cggcgagctg gatcacgaac ggagagatcc cctcccccac ggccctacgg aggacatccc
     1141 gtctctggcg ggccagctcc tcgctggcaa ccatctcgga gacgacgagg ccggcgccga
     1201 gccggtgtgc gagcctgcgg aaggggagat ccgtgacgcc ggacatgggc gccagcacca
     1261 cgcgattgat cagttgacga gacccgatgg cgagcggacc ttggagcagc tgcatggatc
     1321 tcgtgcctat tatttaatca ggtttcattc atgctgaatg gttgggcaga ttaggacagg
     1381 acttcgtctt tcgcaactgc gaatatgagt ttcgcgccgt cgttgcgccc agtgcatcgg
     1441 acggctaagg catctcggcc aataacaaac ggctaaggca tctcggccaa taacaaagtg
     1501 ccatactgta ccagcgccgg aggtgaggtg tgcaggtttc cgtgctgatc gtcgccgcag
     1561 gtcgcggcac gcgcgctgcc ggctcgcagg tgccgaagca gtatcggcag cttcggaata
     1621 agccagttct gacgcgcgcg atcgaggcgt tcgcgaagca cccggcgatc agtgccattc
     1681 aggtcgtcat ccaccctgac gaccgcgcgc tttacgcggc atgctgtgcc ggcatcgccg
     1741 ccgaccttca agagccggtt gatggcggcc caacacgtca ggactcggtg cgtgccgggt
     1801 tgacagctct tgccggagcc gccccggatc tggtcctcat ccacgatgct gcgcgcccgc
     1861 tgatcgaggc ggggctgatc gaccgggtgc tcgcggcgct gagggagcat gacggggcga
     1921 tcgcggcgtt gcccgtgaac gacacgctga agcgatctgg cgcggacggc tccatcgcgg
     1981 agaccgtctc acgcgaaggc ctctggcgcg cacagacccc gcaaggcttc cgttacaagc
     2041 cgatcctcga agcacacctt gcagcgcata cccagggccg atcggatttc acggacgacg
     2101 ccgcgatcgc ggaatgggca gggctgaagg tcgcgctcgt ccccggcagc gcgcaaaaca
     2161 tcaagctgac cgatgaggag gacttcgctt tggcagagcg tctgatcgcg ccctccgaac
     2221 agatggagtg ccggactggt acagggttcg acgtccaccg tttctccgag ggcgatcacg
     2281 tctggctgtg cggtgtccgg atcgcccatg acaaaggcct cacaggccac tcggacgccg
     2341 atgtcggatt gcacgcgctg acggatgcga tcctcggcgc catcggcgat ggcgacatcg
     2401 gccagcattt cccgccatcc gacccgaaat gggcaggcgc tgcctcccac atcttcctgc
     2461 gtgacgccgc ccgccgggtg acggaacgcg gcggcaggat tgccaacgtc gacgtgacct
     2521 tgctctgcga ggcgcccaag atctcacctc atcgcgaggc gatgcgcaac atgatcgccg
     2581 agatcctaga gatcgccgtc gaacgggtcg gcgtgaaggc aacgaccacg gaagggcttg
     2641 ggttcaccgg acgccgggaa ggcatagctg ccatggccag cgccacggtg ctgctgcctc
     2701 cgtgcaaccc ctcctcctga atcgtcggac gtgggagcaa cttaccttgc gtcagcggta
     2761 agaatcgctg atcaaggaca aagcgttgta tctccactgg cgaagccagg agcttccgat
     2821 gaacatcatg acccaggagc cggactccgc cgccgccgag cttgcgaaca tcgcggagac
     2881 gacctcctcg agctgcattg cgacgcgcgt gcgccagctc agccgtatca tcacgcgcgt
     2941 ctacgacgat gccatgcgcc cgctcggcat caccgcgagc cagttcacgc ttctgacaca
     3001 gctcgctcag caggatggca tcaccgcggt cgagatcggc ttcacgctcg acatcgagaa
     3061 gtcgacctta tcgcgcaacc tgaagcggct gctggcactc aacctgatcc tgatggaccc
     3121 gccggcgggg cggcgcggcc gtggcctgca tctgaccgaa aagggccggc gggtaatcaa
     3181 ggaggcctat cccgtctggt tggctgctca gcaacgcgct gtcagcgtcc tgggacccga
     3241 gagccgggcc acgcttgaca ccctccttca tcaggccgag aagctggctg ttgcgtgagg
     3301 ctggtatttc cgcgggtgta ggcgtctcag cgatagccga tcctgtagcc tctcgcccag
     3361 cttttcgagc cggcctctct ccgcgaggga ggaggtgaag gggcgcaggc tgctcgtgct
     3421 tcaatatcga gcggaacggg cctagacttc gcatcgggct gttcgagcgg cgccagtggt
     3481 gcggaggttt catgttccct gcggatctcg tcgacgaagc gcgacagctg atcggcctcc
     3541 tgcgcgacca caagctgatg ctcgtgaccg ccgagagctg cacgggcggc ctgatctctg
     3601 gagtcctcac cgaaattccc ggctcgtctg acgtcgtgga gcgcggctac gtgacttact
     3661 ccaatgctgc caaggtctcc tgcctcggcg tcgatcccca gctcatcgcg acccatggtg
     3721 cggtgagcga ggaggtggcg cgcgccatgg cttcaggcgc gctggcgcac tcgcaggcgg
     3781 atatcgccgt gtctgtgacg ggcgtggcgg gccctggtgg cgggtcagtg gaaaagccgg
     3841 tcggccttgt ccacttcggc gccgtccgca aggggcagcc ggtcctgcat ttggagaaga
     3901 ggttcggcga cctcgggcgc tcggccatcc gcatcgaaag cgtgcggaca gcgctggatc
     3961 tggtgaaagc cgccgtcagg ctcgagtgag agctgggcct tcatcgccgt agatttcgtc
     4021 ggcgcgggct tcgaaggcct cggcgaattt gcggaaggcc ctgtcgaaca gagcgcccat
     4081 catcatgccg agcgccatcg accggaactc gtatgcgatg gagaattcca cgcgacatcc
     4141 atcagggtga ggacggaacc cccatgtgtt ctcaagatgc cggaacgggc cgttgacgta
     4201 gctgacgaag atctcctgct ttacccggtc cagccggacg cgtgtcgtga acgattcgtt
     4261 gatcgggccg tagccgacgg tcatcgtggc gaggatctcc tcggtgccgt cctgcttcaa
     4321 accgcgcgaa tgcagaacga gcttctcgca aagcggcagg aactccggat agcgctccac
     4381 gtccgagacc agctcgaaca tctcccgcgg cgagtgcttc acgatgcgtt gtgtgcggaa
     4441 tgatggcatc ggcagatcag tagcgaacca ttcgattcaa gatatcgcga gtccgcgcgc
     4501 cgaccctatt gcggccaatc atcacgcgcg gggcaaagtg cgtcggacgg gcgcggctca
     4561 cccggcctgc cccatggcgg cgaggcgtgc cgcccggagg cgggcgaagt cgtcacctgc
     4621 gtggtaggac gaccgcgtca acgggctggc ggagaccagc aggaaaccct tcgcataggc
     4681 gatccgctcg taggacttga actcctccgg cgtcacgaac ctcttcacct cggcgtgctt
     4741 gcgcgtcggc tggaggtact gaccgatggt gaggaaatcc acgtcggcgg tccgcaggtc
     4801 gtccatgagc tgcagcacct cctcgcgttt ctcgcccagg ccgaccatga tgccggactt
     4861 cgtgaacatg gtcgggtcga gctccttcac ccgttgcagg agccggagcg aatggaagta
     4921 gcgcgcgcct gggcggatct ggagataaag ccctggcacc gtctccaggt tgtgattgaa
     4981 aacgtcgggc ttggcctcga ccaccacctc gagcgcgccg tccttgcgca ggaagtcggg
     5041 cgtcagcacc tcgatcgtgg tgccaggcgt gcgctcgcgg atggcgcgga tgacggccgc
     5101 gaagtgcccc gcaccaccgt ccgcgagatc gtcacgatcg accgaggtga taaccacgtg
     5161 ctcaagcccg agctttgcca ccgcctccgc cacgttggca ggctcctgtg gatcaagagg
     5221 tcgcggcaat ccggttcgga cattgcagaa ggcgcaggct cgcgtgcacg tgtcgcccat
     5281 gatcatcatg gtcgcgtgct tccgctccca gcattcgccg atattggggc aaccagcctc
     5341 ttcgcagacc gtgtgcagcc cctgctcgcg cacgatgcgc ttcgtctcga catagcccgg
     5401 cgaacccggt gctttgactc gaatccactc cggcttgcgc agtatcggcg tgtccggctt
     5461 gtgcgccttt tcagggtgcc gggggcgcat atcgacgatc gtaaccatgc acgagggctc
     5521 gtgatctcat cagcagtctt agactgcggt tgtctacaag gtggcccgtt cggccccgat
     5581 gatcaaccgg catgtcgccg cggatcggcg acggctgcca tgctcatttg gcaatggcac
     5641 gggcgagcaa tgtcacagca atggccccta tggtggccac tgcgacctga tcgaggaagg
     5701 cgctgcccag cccgaagctg aagccgagca ggccgtggat gacgtatgcg ccgacgagcg
     5761 cgccgaggca accgacgatg agatagcgaa taagtccgcg ctggccgaac agcatgccgg
     5821 tgatccagcc ggccaccaat ccgaccacga tgaacgtgag ccagggagaa tacgtcccga
     5881 aatccaacgc gtcttctccg tgtcgctgcc ggcacgccct atggctaact cctgtctgat
     5941 gatgtcacga ttcggctgcg cttggcggca cggctttcag ctctcacaaa cgtaggccac
     6001 ggtgcggata cgccggtccc gcaagttctg caccaggttg cgagccatca aggggaaatc
     6061 attcccaagg aacgtctgga atgtgacggc tggagctgat tgagccattc gggcaaccat
     6121 cgcctcgaac caacgcgcgc tctcctcagt tgagtccacc acctgcagga ccgtcattcc
     6181 ggcacctgtc agcagcaggg acatttcatc gggcgtagcc agatggctga tcgacggatc
     6241 gcgcgcccaa gggaccggga acagcacctc gccgccttct ccctgcagga catcgtaaac
     6301 gccaaaccgc gcaccgggct tcaggacgcg cttggcctcg gcatacatct tgtccttcgc
     6361 cgcgatattc atggctacgt gaatcgtaac ggccgcgtcg aactcggcgt cgccaaacgg
     6421 cagatcggtc gcgtcacctt gccggaatgc gacgcgatca cccaaaccaa cccagttact
     6481 gagagtggtg gcggcttcgc agaattctgg ggtcagatcg atgcccgtca cgcgacagcc
     6541 ataggtctcg gcgagagtcc gcgccggccc accgagcccg ctaccgatgt cgaggacgtg
     6601 cgacgttgca ctcagattca atgactgagc gagctcgagc gtggcctggc ggccgcgaat
     6661 gtgaaattca tcaacggtcg ccagatcggc ggtggtcagg ttgtcgatga ccttcccggc
     6721 attgcgcagc ctgtccgcga tcgccttcgc caagtcgccg ccgccagcat agtggcgtgc
     6781 caccacgtcg ctcatcgtcg cctccggcca tcgcggatga tctggccatt atggcatgag
     6841 cgaagtgcgg gcagaagagc gtccgatcgg gaggtctacc ggatcaggta tgaatcaccc
     6901 ggccgaaggc gtcgagcaca ctctcgtgca tggtctcgga gagcgtcggg tgcgggtaga
     6961 ccgtgtgcat caggtcctcc tccgtcgtct cgagttgcat ggcggtgacg aagccctgga
     7021 tcagctccgt gacttcaggc ccgaccatgt gcgcgccgag cagctgcccg gtcttcgcgt
     7081 cgaatatggt tttcgtcatg ccctccggtt cgccgagcgc gatcgccttg ccgttggcgc
     7141 ggaacgggaa tcggccgacc ttcagctcgt agccagcttc cttggccttt gcctccgtca
     7201 ggcccactga ggcgatctgc gggtagcaat aggtgcaccc cggaattctc agcttgtcca
     7261 tcgggtgggt cggcagaccc ttgatggtct cgacgcagag cacaccttcg tgctcagcct
     7321 tgtgggcgag gagcggcggc ccggcaacgt cgccgatggc gtagataccc ggcacgttgg
     7381 tgcggccgag accatccgtg acgatcgcgc cacgctcgac tttcaccccg agcttctcga
     7441 gccccaggtt ctcgacgttg ccgaccaccc ccaccgcgga aatcaggcgc tcagcggtga
     7501 tctgctcgct cttcccgttg ccgagatcca cagtggctgt gatgctgtct ttgccctttt
     7561 ccacctttgt caccgacgcg ccggtcagaa tgcgcattcc ctggcgttcg aactgcttgc
     7621 gggcgaaagc ggcgatctcc gcatcctcga cgggcaagat ctgcggcagc aactcgacga
     7681 ccgtcacctc cactccgaac gtgcgataga acgaggcgaa ctcgatgccg atggcgcccg
     7741 accccatcac cagcagcgat ttcggcatcg caggcggaac catggcctcg aagtaggtcc
     7801 agatcagctt gccgtcgggc tcaatgccgg gcagcacgcg cgggcgcgca ccagtcgcga
     7861 cgatgatgtg cttggcctgg taggtccccg taccgagcac gcctttcggc ggagatatgg
     7921 cgggctgcat cggcttcttc ttcgtctcgg acacgacgag ctcgcccggc ttgctgatcg
     7981 tcgcttcgcc ccagatcacg tcgatcttgt tcttccgcat caggaagccg acgccgctgt
     8041 tgagctggct ggaaacgccc ctcgaccgct tgacgattgc gccggcgtcg aacccgagct
     8101 tcccctcgat gctcaggcca taagccccgg cgtgctccgc gttgcggtag acctcggctg
     8161 acctcaggag cgctttggtc gggatgcagc cccagttgag gcagatgccg cccaggtgtt
     8221 cacgttcgac gacggcagtc ttgaacccga gctgggcagc ccgaatggcc gccacgtaac
     8281 cgccggggcc agtaccgatc acaacgacgt cgtaggaatt atccggcatg acgatcctgt
     8341 cagatgagag agtgcgccca aaataaaagc tccgcgcgcg agcccggagc cttcatacat
     8401 gcgcaagcga tctttcgcta cctgggcctc gcaaccccta cgaatcagac gtggcagcgg
     8461 gccccgagat acacgttcag caggtaatta tatcgatcga cgtcggcctg agtctgttgc
     8521 gacagcttct tgcccgcgct cgcggcctcc accttgggac gaattccctt ggcgtcgagc
     8581 ctgttcagct ctgcacgtac cgactggcag ctgtaacccg caggcagggg ccgggcaccc
     8641 gggtcgccat aaggcccagc attgctcgcg cagccggcca gtgcgacgac ggccgccaat
     8701 atggccccat gtttcaagac cttagttgtc ccccgtcgaa ccaatgtcgc ctcccggccc
     8761 agcgctaacg aagtgcaaat cgtgttcacc acagtcacgc tgctttcgca agtcccagcg
     8821 cagcaatgca gcaacagagc ctgtcgattt cgccgctcat attcttgtga agtctccagg
     8881 tggacgctga ggatacagtg ccctgcgcaa gatcaatccg tagtgcgatt gaccaccacc
     8941 tcgccgcgat cccgccttcg cgcaaactgc gtcacctggc ccgaggcgag catgcgggcc
     9001 tgctcgatcc agttctcccg ctcgatctgc cctttgacct cgagcgcctt gctgacccgc
     9061 gccacatcgg ctgccgtcca gttcgcgatc tgctcatagg tccagtagcc catgccgttg
     9121 aggcgcttct cgaacagcac gccgatgccg cggatgcgct tcaagtcgtc cgggcgcgcg
     9181 gccagccgct caccaggacc gatgccgacc gcgctctcct gtttggcccc gacgagagcc
     9241 tcggagcgga ctgaacgaag cgtgctgagg tcgaccttgc gcggctggcc ctcttccggc
     9301 ctgggcggcg gtgatgcacc atcggtcttg gcagcctgcg cctcgcggat cgcctcggcg
     9361 agctgagtcg ggcgcgggcg cgcggacgca gcttgcttca gatcgaactc gcgcgcgtag
     9421 gcggtttccc ccccactggc gaggaggtgc gcctgctcga tccagttctc gcgcgagatg
     9481 cggccatcgc ttcccatgag gtgctcgaag cgcgcaacgt ccaatgccgt ccactgcgcg
     9541 atctgttcat atcgcgtcac cccttgcgca ttgagcaggt gctcgatgtc ctcgctgacg
     9601 cgcctgatac gctgcaggtt atccctgccg gccgttgcgg cggcacgtcg cggttgtgcg
     9661 gcgctgacgg cggcggccac ggcttccgca acgccggatg cctcggtcgg ctgcgagaca
     9721 gatgcggccg tcgcagcggt tgccggcatt tcatcacctt ggtcggccgc gggcttcgca
     9781 acgaggccca cgtggccgcg gcgggaaaag gcggtttcat tgccgcccat caggatctgc
     9841 gcctgctcga tccagttctc ccgctcgatc cggccgcgga agccgagggc ctgactgatg
     9901 aagcgcacgt cctctttccg ccacgctgcg atatcggcga accggcgcac gccgagcgcg
     9961 ttgaggcgct tctggagcgc ggcgtcgatg gcgcgtatgc gggtgagatc atccaagccg
    10021 gccgcaggcg ccgccggggc ggattggttg gtggtcggag catgcatgcg cgggtcgacc
    10081 gtactcgcct gccccgatgc gccggaagtg gacgggatgg ggtgcgccct cggctcggca
    10141 tcggccgatt tctcgcgtgc cgccgcctgg cgacgggccg ttgcggcggc caatgctgcc
    10201 gcggcagcgg cagacgtccc gatggacctg gaggaatgct cgagccgtgg aaccgcgggc
    10261 tcctcgcgcg caggtgttgg agctgatgct gctgggctcg ttgctacctg tgcgggaggt
    10321 gcgtgctctc ggcccggggg tggtttgacc gctggcgctg aactggacgg aacagaagct
    10381 actcctccat caggttgtcc ttcccggccg ctttctgagg cggatgcagg caccacgccc
    10441 acggatgcct cgatgggcgc tccgccggct gttgctgcca taggaggcgg atcgggcagc
    10501 cgctgcggcg gcactggtgc gacggtcccc cgaggggcga tccccgccgg ggccctggcg
    10561 aagaacactc gcctgatgag gcaacccgtg aaggtcccta gaaaataggc acccagcagc
    10621 aacaggccgg tctcccacag cagagaagtc atcggtgatt ccccaatagg cgcaactcgc
    10681 tccgcaggag ccggctatca ctctttgacg cgatagcagg agacccagcc gacgaccagc
    10741 ccgagggcaa acgccagcaa tacgtaccag aacagcttgg caagcaggaa atccatgtcc
    10801 ctcgttcccc cttccgaccc ggtttcggcc gatcactcga tgatgaggat ttcaacccgg
    10861 cggttcatgg cgcggctctc cggcgtatcg tttgccgcta cgggccgtgt cgcgccgtag
    10921 ccgacggcac tgatcttctc gatgggaacc ccgcgactga ccagataatc gaccgcggct
    10981 ttcgcgcgct cttccgaaag cacctggttc ccttcagggg agccctccgc gtcggtgtgg
    11041 ccttcgatat gaatgcggac gttcgggcag gagcgcgcga cttcgagcag cctcgaaagc
    11101 gtctccttgc cggtcgcgtt cagttttgcg ctggcgcggg cgaagttgac cgtcccgtgc
    11161 agcgtcgcgt tggcgatggc ccgttgacac cgcctgtccg cctccagatg ctccggagca
    11221 accgcggcat gctgctcggc cgccgcgtcg gatggaggct gttgaaccgc aagagccacc
    11281 tcctcggact cgggcttcgc cgccgaagcc tgcttcgcgt ccgtatcgtc cgagccagga
    11341 gtgtgctgat ccgcggcggc atccgcctcg gctcggcgac gggcctcctc ttcggcgtgc
    11401 gcccgagcct cggcttcggc cttcttctgt gcttcggcgg ctgcagcggc ttcctccgct
    11461 gccgcggccg ctcgggcctg ctcttccgcc ttgcgcagac tttcctcctg gcgcaggcgc
    11521 tccgcttccg cagcaaggcg cgcttcctcc gctgccgcag ccgcccgggc ctgctcttcc
    11581 gccttgcgca gattttcttc ctggcgcagg cgctccgctt ccgcagcgag ccgctcttcc
    11641 tccgcctttc gcgcttcctc ctccgctttc agctttgcgt cctcttcggc tttgcggcga
    11701 gcctcggctt cagccttgcg cttggcctcc tgctcggccc agatcatcgc gtcggaacgg
    11761 acctcgatgg aatcgatgcc cctgtagccg ctgggcaggt ccttgagctg ctgctggatc
    11821 agggtgacga cggccgcatc gggcgcttgg ccgacgatcg acaactcgtt gccgagaata
    11881 cggacctcac caccacgcag ccgcgccagg agcctgaggc cggtatcgct caccttcgcc
    11941 cacttctccg aacgcgcggc cttgatctcc atccggctta cgatgtgcgc ctccggaaac
    12001 agcttcgcag cggcctgctg gagctcctct ttcgtcttct catccggcac gtccccttcg
    12061 aggacaacct gcttttcggc gaaagacgca cgccagctga gatcaggctc gggcggggta
    12121 tcgacgacga gcctcgcgtc cagctcgcag gcccggttga cagccgcgcg cagctcgttg
    12181 cggaccgctt ctgcgacctg ctcatcctcg gtgacaccgg cgaatatgag cttgttgtcc
    12241 accatctcga tgcgcccgtt gccgagcttc acgagcgagg ccattccgga cgtcatgcag
    12301 gtttgccagc cgtccggcgc attggctccg agcttgagac cgtcggatat gtcgagcttc
    12361 gggaagtagg acttcgctgc cttcacaagc tccgcgcgcg cagcctcgct cggcacatat
    12421 cccgtgagaa ggagcttgtc gttgtcgatt gcggccccga tggtgtaggg gctcacgggc
    12481 ttgatggccg gctcgcggaa cttgatcttc tcatccacct tgatggactt ggggattccc
    12541 tcgcgcagcg tctctcgcaa gaaattcgcg gtctcctcct tggtggccag ccccgatatc
    12601 agcagctcct tgcccttgat ctcgatgtca ccttgctcca gttgggcaag cttgccgagc
    12661 gcacctacgg cggcgctcac gaggccatcg ggctcacccg ccgcaatacg catccggtcg
    12721 acgatcaccg ccttcgggaa agcctcctgc gcagccgcga acacctcctc gcgtgcatct
    12781 tcactcggga cgtagccgcc gagctgaagc tgcgtgccag agaggcgcgc tgcccagatg
    12841 tagggatcga caacgggtgg cttgaccctg tcctgcttca gcgcaatgcc ctgaggcagg
    12901 ccggttgcca gggctgactt gatgctccgg aaagcggcgg aatcctctgc ttcgccgacg
    12961 atctccaggc cgtcggactc gagctgaacc gccgcacctg gcttgagccg tgacagctgc
    13021 ttcaggctga agctgaccgc gccgagccag atctcggtgt ccggcgcgcc gcgcgcgagc
    13081 ttcatgcggt cctgtacctc gagctgcgga aaagtcgcct tggctgcgcc gatgatcgcg
    13141 cgccgcgtct gctcgttcgg cacgaagccc gtcagcttca atcgatcgtc acgcagctgg
    13201 gcccgccaga cgtagttctt ctcctcggcg atgaggtcag tgcgattgga gaccttgcgc
    13261 acgccccaca gggagctggt gatctggtaa gccttgcgcc tctcgttctc atcgggcgcg
    13321 attccgagga gcttgctgtc tcgcccggag aattcgacct cggcccagtc gagcccagcc
    13381 tgcgcgaggg attggcgcgc ctcctgcgcg agctccgctt cgatttgggc gaggacgccc
    13441 tgcatgttca tgacgccgag caggccaagg accaggacgc ttcccaccag ccaacgcaag
    13501 ggattgcatc tcatgccgct cttatctccc tcgtccccgc aatgggcgcg cgcaatgcct
    13561 cgaccgattt ctcgcttaat cgtcgggcta gaacgtacgc aagcgcgact gcgatcgccg
    13621 cgttctgccg tgcccaccca atgccgtgga aactatagtc aagcggcatg cccaatggaa
    13681 gctggacaga agacgcgttc gctcacgcgc aagccagcaa tggtcgcgtt ttcaacagaa
    13741 tgagcaacat ttgaacatca caatttgcac atacttcgaa atatcgctca cgactgatgc
    13801 cgcagaatat cagatgtacg cccattctga tccggcagcg acttgtcttg ctactttcgt
    13861 acagggcgtc ggaggcgccc cagttcttgc tttcgtcgaa atattgacaa cgcccatccg
    13921 cctcctatcg tgcgttcaat cgccccatcc ggcactggct ggcgtttgat tgaggatcgg
    13981 gcatcaactc ccgggagcaa atccgtgcaa gacgttccga cgaagctgaa gcagtaccag
    14041 acgtacatcg acggcaaatg gtgtgatgcc gcctcgggca agacattcca gagcagcgac
    14101 ccctacaccg gcgagccttg ggctctcatt ccagagtgcg atgccatcga cgtggaccgc
    14161 gcctgcaatg ccgctcaccg cgccttcgag agtggtccgt ggccggccat gacgcagacg
    14221 gcgcgcggcc ggctcttacg caagatcgcc ggcctcatcg agaagcacgg ggagcatctc
    14281 gccaaaatag aagtgaagga caacggcaag ctgatctcgg aaatgtccgc tcagctcaag
    14341 tatctgccgg agtggtacta ttactatgcc ggcctggcgg acaagatcac cggcatggtg
    14401 atccccgtcg acaagcccga tcacttcacc tacaccctgc gcgagccggt cggcgtctgc
    14461 ggtctgatca tcccttggaa ctctccgctg ctgctggtcg gcttcaagct ggcgcctgca
    14521 ctggcagctg gctgcaccgt cgtcatcaag ccgtcggaat acacttccgc atcactgctg
    14581 gaattcgtga agctggtgat cgaggaagcc gacgttccgc ccggggtggt gaacgtcgtc
    14641 acgggatatg gcgccacagc cggagaagca ctcgtcaccc accccaaagt ggccaaggtg
    14701 gcctttaccg gcgggagcgc gacgggctcg cgcgttgcct cgctcgccgc ggcgagcttc
    14761 aagaaggtgt cgctcgagct cggcggcaag tcgccgaaca tcgtcttcga cgactgcatc
    14821 atggacgagg ctgtcaacgg cgcgatctcg ggcatcttcg ctgccaccgg ccagacctgc
    14881 gtcgccggct cgcgcctgct cgtacaggag agcatccacg acgcgttcgt cgagaagctc
    14941 gttgccacgg cgaagacagc caagctcggc aatccgatga gcttcgagac gcaggtcggc
    15001 cccgtcacca cgccaccgca atatcagaag gtcctctcct atatcgacat cgccaagagc
    15061 gagggcgcaa actgcgtgct gggcggcggt ccggcgggcc ctgagatggg tggcggcaag
    15121 tacttcgtgc agccgaccat cttcaccggc gtcgacaaca agatgcgcat cgcgcaagag
    15181 gaggtcttcg gccccgttct ctcaatcatc aagttcaagg atgaggagga cgcgatccgc
    15241 attgccaacg acgtgatcta cgggcttgcg gccggtgtgt ggacgcaatc cgtcaagcgc
    15301 gccaccatca tgccgcggcg tatccgtgcc ggcaccgttt gggtcaacac ttatcgagcg
    15361 atgagcttta cgatgccgtt cggcggcttc aaaagctcag gccttggccg ggagaacggc
    15421 cagaccgcca tcgacgagta tctcgagacg aaaagcgtct gggtgtcgac cgcctcgaac
    15481 acaggcaacc cgtttgtcat gaagacatct gcgccgtcct gatgtcggcg aacgggctcg
    15541 ggagtgaggg cgcgcccgtt cgggccctcc catcactccc gtaaccacct ctcttcctgt
    15601 gggccctcag gagagatcag gacggatgtc agcacatatc cctggctccg tccggaactg
    15661 aaatcagtgc ggacgcccgc tcgcgcgcgc cgggcaatgc gcaaccgcct cgcaaatcga
    15721 cgccggtgac aggatcttgt cgcagctctt gagcctggct ccgaggcact cggcaccttc
    15781 gaccacatcg tcgatggccg aagtctggag cagagcccag gcacacgcac gcatcgccgg
    15841 acaggccccg tgctggcttg ccgccaccat ggcaatggcc atgcactcgt cgcggcagaa
    15901 cccggcacac tggccggggt aaatctcgat ctgccggcag gagcagttgc ggattgcccg
    15961 tacccagcag gcaagatccg tcactgcgga ccgcgcttgc gtgggcccga gcgcgttgga
    16021 aaagatcgac caagcctctt cccagcagga gatgtcacct gtctggtagc cggcgagcca
    16081 ggaccggaat cccagtccga ccagccgctc gggctcgttc aagcgtgacg caagcggggt
    16141 tgccgcggcg acggccccgg cgcgcggccc cgacgagaat ttcttgcgga gcatcggacg
    16201 atcctgtcga tgaatgtgag cacaatagcc cgtggcctcc tctcaagagg ccacggggtg
    16261 caacaggctc gaccactcag gacgacggcc ccgcgtcgaa gtcgacctta tcgacaagct
    16321 ccgaagcttt cttgcgcaag ctcgcgatcg tctcgatcgg cagcgtatcc ggcttcagcg
    16381 cgccccagga ctgcacgatc tttgatggct tggcttcggg gctgatcggg tattcgttgt
    16441 tggcctgcgc gtagatctcc tgcgccttcg gtgaggctaa gaattccatc agcttgatgg
    16501 cattctcctt gttgggggca tacttggcca gcgccatgcc ggagatgttc acgtggctgc
    16561 cgcgatccgc ggcgttgggg aagagcacct tgatcgcatc cgcccaggcc ttctgctctg
    16621 ggttcttcat catcagcgcc acgtagtagg tgttgccgat ggcgagatcg catttgccgg
    16681 aatagacgtc acgtgcctgc tcgcgatcac ccccggacgg cttctgggcc aggttttcct
    16741 tcaagcccct gagccacttc tccgcctccg cctcgccgtg gtgagcgatc atggaggcga
    16801 tcagcgagat attgtaagga tgctgccccg accggctgca gatgcggccc ttccattttg
    16861 gatcggccaa ttcctcgtag gtgatgctgt cctgcgacac cctctccttg gaggcgtaga
    16921 cgacacgcga acgcaaagtg agaccgaacc agtgcccttc cgggtcacgg taggcggcag
    16981 gaatggcctt ctcgagcgcc tcggacttca ccggctgggt cacgcccatc gtcttggcct
    17041 cgttgaggcg cccaatgtcc actgtgaaca agagatcggc ggggctgttg cgtccctcgg
    17101 cggccaatcg ctcattgagg ccgctgcggg aatgcacgac gttcgtcttg atgccggtct
    17161 cctcggtaaa ggccttcatc aacggatcga taagccccgg ctcccgatag gaatagatat
    17221 tcacctcgcc gtcggcatgc gcggcaagcg ggaaaagggc gattgctgca agtgagaagg
    17281 aggcatgcgc gaatgcgcgc agggcccggc gacccttggt cgagtaggtc atcgttttct
    17341 ccaatgtgcg aggttcaagg cctcgtatcg gaactgacgc cggatttgtc cggcggacga
    17401 tcaattctcc aagatcaacc gagctgtcaa ggtggagacg ccaaatcgcg acagaaaatc
    17461 cctaagtcga gggtaattct ggcaatcttt gaattagaac tattcttagt ctgtattccg
    17521 atctgtatga tcctctattg cgccacactc gtttccgccg tgacggtggt gatttgccgg
    17581 attgcaatag gttcagagaa gaaattcgtg ctaatgtgaa cgaaatccca aaaatcggag
    17641 gctcgcccat gaccaaggat tccccggtca cccctgcgtc tgacgattgg cggccggcgg
    17701 cacgctatcc cgacccggcc atcacggcgc tcgacccgcg gttcgaaaac tattggctga
    17761 agctcgcgtc ggtggagcgg atcgccaccg gctgccgctg ggccgaaggc ccggtctggt
    17821 tcggcgacgg gcgatatctg ctctggagcg acattccgaa caaccgcatc ctgaaatggg
    17881 aggaggaaac cggcgccgtc agcatctacc gcaagccgtc gaattacgcc aacggcaaca
    17941 cgcgcgaccg tcaggggcgc ctcatcacct gcgagcacgg cggccggcgg atcacccgca
    18001 ccgagtatga cggtagcatc accgtgctga tggacagctt cgaaggcaag cggatcaatt
    18061 cgccgaacga cgtggtcgtc aaatcggatg gatcgatctg gttcacggat cccgtctttg
    18121 gaattctcgg ccattacgag ggccacaagg cgacaccgga gctggaagcc aatgtctacc
    18181 gcctcgatcc agaaacgggc gaggcaacga tcgttgcgga aggcgtgcta ggcccgaacg
    18241 gcctctgttt ctcgcccgat gagtccatcc tctacatcgt cgaaagccgc ggcatcccga
    18301 accgcaagat cctcgcctat gacgtgatcg acggtggacg caagatcacc aacaaacgcg
    18361 tgctgatcga cgccggcccg ggcggcacgc cggatggcat gcgctgcgat gtcgatggca
    18421 atctgtggtg cggctggggc atgggcacac cggagctcga cggcgtgatg gtgtttgcgc
    18481 ccgacggcac gccgatcggc cgcatcgccc tgcctgagcg ctgcgccaac ctctgctttg
    18541 gcgggctgaa gcgcaatcgg ctgttcatgg cggccagcca ctccgtgtac gcactctacg
    18601 tcaacactca aggcgccgtt ggtggctgat tgcaagctca gggcgttgtt gcgagcgcag
    18661 cgatgcttgt cagcgccgtt gcggctcccg gaacgagcac tccggcaagg agcagcgcga
    18721 cggcactggc tggcggcagg tacgaccaga tcgacagcat cgcaatcact ccgagaaggc
    18781 gcacgcctgc tcccgccgcc tgagccagtg cagcgtgcat caagcctgcc aatgtttccg
    18841 ccccgaggat cagggcgccc aagccgaagg cagcggccgc catgatctga aggcagccgg
    18901 cgacctgaaa tatgactgcc gcaggtgcgg ggctcagtcc tttgaagatg atatcgacag
    18961 ctgcaccgaa gctgatgatc ccggtcaata aaagcaggac aggataagcc acgctggccc
    19021 ggatgaacct gcgcgcaagg agctggacac tggagctgca gatgagaatt gcgagcgcag
    19081 cactgcggcc cggcagcatt ggctcgtaat gcagcatgag atggatattt gcggacaggt
    19141 ctgctggtat ggagttgaag gcctccgcca gaagcaccaa ggcgccggcc tcggcgaaaa
    19201 gcagccccac agcgagggcg atcggaaaag cattgctcaa gaaattccga ttgcaatttt
    19261 ccggctcgga ttcttttcgc gccttgcatt ccagcacgag aacgtggctc gatctgcttt
    19321 gcgtgaagaa gcgaaacaag attgcgagtg aaatgacata ggccgcgact aaaggcagta
    19381 cgacgagaac tctgcccatc ataacagctt catgcagacc ggtatcgcct ggttttgtcc
    19441 catccgaaac tcagtccgaa aaggcgatct cgtatgagca gggcggcgcc ggcagtcgac
    19501 gcaagaaggg atagacagga cgaaacgaac agcagcggga gcacacggag aacatgcctc
    19561 tggccgtcag aatgggcagc gacgatcacc tgggcgtgtg gcgcaagcag cgcaattccg
    19621 aaaagcgagg caatcaccca cccgatcccg tccggggtac ttttaccgcc cattgcaagc
    19681 agcggaacgg cggcgaacat gctgaccaga gccagtacgg gaaaagcgta gaaggtcaga
    19741 ataagtagcg cgtcgagctt ttggccgagc ctgaggcgct ccgagcgcaa gatggcgccg
    19801 aaatgatcga gcaggcactg gttgtggcca taggcccaac ggcggacttg acggaaccgg
    19861 ctgccccagt cctccggaac ctcctcgtag cagcgtgcct gacagacgta tgccacgcgc
    19921 cagccgccaa gcaccagacg gaacgtgaga tccgtgtcct cggccagatg gcccacattc
    19981 catccgccga cgctgtcgag cgcggcacgc cggatgcccc ctaccgtgcc gccgaactgc
    20041 ggaacgaggt ccagcagcgc ccgaccgttc tggtccgtcc catatcctgc ccgtcgctcg
    20101 agatcgagga gtttcgtgag tagattgcgg tcggcgttga gcgggaccac ccgacccatg
    20161 gtcgcgccaa cgtccggatc ttcaaacggg cgtacgagct ctttcagcag acccggcggc
    20221 ggcagataat cggcatcgaa aagaacgatg atatccgaac tgcttttcgc catcccgtcc
    20281 gcgacggcag cagccttgcc gggtttggca gtcgcgtcgc ggtgcaacac cgcgacgcga
    20341 ggatccttct cgacgaagct gtcggcaatc cgtccggtac ggtcctccga gcgatcgtcg
    20401 accactaaaa tcgaaagctt tctcgacggg tagtccagcg ccgtcatcgc tgccaggctg
    20461 cctgcgatga cgcgttcctc gttgtgggcc gggatcagaa ccgtgacgct cggccagtcc
    20521 caattttctg gaatacggga tgggtgatcc ccagcgctcg gactgaaacg ccaaacgcac
    20581 cagccgagga gcaactgacg cacagcaagc gccagcccca gcaaagccgt aagcagaatg
    20641 aaggcttcga ggaggagcat cgccggcggc ctcgaggttc agggttgatc gaaatgaacc
    20701 ttgagaccga cgtggccgcc ataaacatcg tacgactccc ggctgctgta ttccatcagg
    20761 ccgacgagct cgatgtcatc cgtcagatag aacgagacga ccgggcgaat gctcacatac
    20821 tgctcatcga actgcccagg agcaaggacg ccgtcgtatg tggcacggcc gccttcgaca
    20881 tggacaccaa ctgacacatt tctgggatga acataggcca ggccggctcc aaacgcgctg
    20941 ctgagatgat cgccggggct cgcgtggacc acgcgcgcga gagcgctcaa gatcagattg
    21001 cctgtctcaa gcggaatgta ttttgcaatt tcgatctccg ctgcggtttc ttcagcactg
    21061 tttctgaaac tccggtaggc caaggcggaa tgaaagacga ggcccgtttc ggcgctcgtg
    21121 cggaatgttc cttccacgcg gccataaagc tccggaagaa tgctatcgtt ttccgagctg
    21181 gtcccgagcc agccgcgcac ctcggcagag tcgccgaccc atgaaaggcc gccggcgaaa
    21241 aagcctgcgg tctcctcccg atccatgtag tgggcttcgg catgcactcc gaggttattc
    21301 gagaacaagg tatttcccca gaccgttgta aaccgccggt cgctgatgcc gtggctcacg
    21361 ccataatagc caaatacggt ggtgatgtcg gagcgcgtta cctgcatgcc ctgtgccatg
    21421 gcgctcgtcg acaggagcag gccggaagcg aaaagcagtc cagctactat ggcgggcatg
    21481 caaaacatac gtatactcac actcagcaga atgccggacg agcgcaactc ccgcacggtg
    21541 cgaatccgtc tagctcgtcc ggcaccctcc ccgctgcaac ctgatgcccc cggctacgcc
    21601 gaagctgtaa cgctctgcca cctcttcaac caggcctggc ggggcatgcg atgcattatc
    21661 ctggccgccc ggtatggacg tggccttttc gcagcctcca cctccaagtc ggagcagaaa
    21721 ttggaaagcc cttccgagag gacggcttgg acgatccgct cggcggcgcg cccatcgccg
    21781 aacggatttg ccgaatgtcg cgaggtgagc ccagtttcct tgccgtcgag aattgccgag
    21841 accgccgcga tgatgctgtc gggatcggtc ccaacgaggc gcgctgggcc cgcctcgacc
    21901 gcctcgcgcc gctcggtgac atcccgcatg atcagcacgg gtttaccgag gctgggcgcc
    21961 tcttcctgaa tgccgccaga atccgtgagg atcaggctgg agcgcatcat gaggtagacg
    22021 aaggcgccgt agtcggcggg cccgatcaaa tggatattgg gccgtccacc gaggcgctcc
    22081 atgactggaa cccgcacctg cggattggga tgcacaggga acacgatcgc cacatcgcgt
    22141 cgttcgcaca gcctgagcac cgcgcggcag atcttctcga tgccttcgcc catattctcg
    22201 cggcgatggg ccgtcaccag aacgacatca cgggcgccat ccagaaatgc aaattgcgat
    22261 tctaggcggg ctttcaatcg cccatccgtc cgcaaccgcc ccgtcgccga gcgaagcgcg
    22321 tcgaccaccg tgtttccgct gacaatgatg cgatctggcg gaatgccctc ggcgatgagg
    22381 tttcgtttgg ccactgtcgt cggcgcgaaa tggagcccac tgctaagcgt aatcagccgc
    22441 cggtaccact cttccggcca gggttcgtcg aggttcccgg tccgcaagcc cgcttcgacg
    22501 tgaccgaccc tgattttccg gtggaatgct gctattgccg cagcggctgc cgaggtcgtg
    22561 tcgccctgga cgagcaccat gttgggctgg gtttcggaaa aaatccgctc caattcaccc
    22621 attagggcgg aaaaaagctg gttcaatgaa ccggcttttt tcatgacgcc caaatcaaaa
    22681 tcgggcgcga tcccgaaaaa accaaaaatg ggataaacta tttgctcatg ctggccagtg
    22741 aaaaccgtga gcgattcgac ccccgctttt gcattgtttt ggagcatttt gatcacgggg
    22801 gccatcttta tggcctccgg tcgcgtgcct atgatcgaga gtacgcgcat acgtgccccc
    22861 tcccacaccc tggatcgacc aggacgtgtc gccaaaacac gggttaagcg gaaggttgtg
    22921 gtgccgaacc tttcctgcag gactcccctt ccctgcagga aacaccccca ttcccctagg
    22981 cgtgagcata actgcaacga cgtgggaaaa tcaacagggt ttctcgaccg gaagaatgca
    23041 aaataaacag agtgctgaat gcaaaagttt tcaattattt aattgtcagg cgcgcaaaat
    23101 tcggtcgagc ttcccagccg ttttcggaaa tagacagctt acgtcgtttc tcaagcggcc
    23161 gatccgttgg cgccccactt ttcggtctca atcgccggcg atctccgcca gggcggagcg
    23221 cattcggccc gatatgggaa ctggccgccg ggtctgtcgg tcgacgaaga cgtgcacgaa
    23281 ataaccatcg gcgcgagcct ctggctcacc caggccgaaa agcccgatct cgtagcgcac
    23341 ggacgacgtg cccaggtgcg caacccgcat ccctgcctcg atgtcctcag gataagcgaa
    23401 ggagcgatgg aaccggcagc tcgtctcgac gacgatgccg atcgtttggc cggtatggat
    23461 gtcgagcccg cccggatcga tcaggtaccg gttcaccagc gtatcgaagt acgagtagta
    23521 gaccacgttg ttcacgtggc cgtagaggtc gttgtccatc caccgcgtcg ggatgatgcg
    23581 gaaatgcttg aaaatcgagc ggtgggcagg aggcctgcgc tgactgctgc tcatgagcga
    23641 tcccgtcgtg agccggggag caacgctccc cggggcaact tgctcgcgcg ggttacttcg
    23701 cggcgataac ggcgatctcg accaacagct tggggtcggc gagcttggcc tcgatacagg
    23761 cccgcgccgg cagatcgttg ccgccagtcc aggccttcca ggcctcgttc atcggctcac
    23821 gattgcgaat gtccgtgacc cagatggtgg ccgagacgat cttgctcttg tccgtgccgc
    23881 acagaccgag caggcgatcg atctggttga ggatctgctc tgtctgaccc ttggcatcga
    23941 ggttccggtc atcggccacg ataccggccg tgaacacgaa cccgttggct tcgaccacat
    24001 tggacaggat ggcggtggac tcgtggcgcg taatggtcat gctcatctcc tgcgtggttg
    24061 gataggtccc gcaaccggtg ataccacgct tcagccggcc tgccagcccg cgcggatgct
    24121 cggcggcctt gacccacagg cgtttcggtc ggggctgctc tcagtcagag cttgtgcgcc
    24181 atgcgctcac gcttggtcgc cagataacgg gcattgtacg gcgacggcga agtggcgtgc
    24241 tggatctgct cgaccacgtt tatgccgtgc ttctgcagcg cctcgacctt cagaggattg
    24301 ttggtcaaca gacgaatgtc cgtgaagcgg aggtctttga ggattttcgc cggcaactga
    24361 tagtcacgcg catcgaccgg taagccgatg gacaggttcg catcgaccgt atcgaggccg
    24421 cggtcctggg cagcatacgc cttgatcttc gccaccagcc cgatcccccg gccctcgtgg
    24481 tgaggcagat agatgatggc gcccgtcttg ctctcgatga ttcgccctat ggccttctgg
    24541 agctggaggt agcagtcgca gcgcagcgag tggaagatgt cgcccgttac gcagccgctg
    24601 tggacacgca ccagcggcag ctcgtccgat ccgttcgcag ctggactgcg gtacaccagg
    24661 gcaagcgcct gataggaagc ggagtcgtcc tcataggcat acgcctcgaa catgatctcg
    24721 cggccttgat agtcgaccgg aagaaccgtg cgcgcggtat cccggaactc gggcatatcc
    24781 ggctttcctc ccccctcgcc cggacccgga cgggtgccgt tcacctgttg cctgttcccg
    24841 gagcgccggc gcagccaagg tgcattgata ccgtgcgcga tctgtgccgc ggtctcttcg
    24901 gtacttccct tggccaaatc tcgcgcgaaa gtctgcgctg gaactggagc gtctgccata
    24961 gtgcgccctc gattgataac tcagctaccc gatccgggca tccggaagag cggcggagcg
    25021 gtcgggatgg ttccggtcac atgcgttgcg gatcgtcctg cggccggaac acctgaagaa
    25081 tagcagcaga ttcgtcggtt gaacccgcac cggatggctg ggttctgtct cctcggttgc
    25141 ctgggcccaa tcacccctcg cgtcatccct gacaggttag cgccgaatag tgtggcgcga
    25201 atagggtcga tctccggttg catatgcgca gattagacat ggccaaggtg gaccagcctc
    25261 ccttgttgca tttgccgtgc gtcggtcgcg gaaatgtctg aagcggcctg cggtcggcat
    25321 ttgtatcgaa tgccaaccat gggagaaata ggcggcggcg gacaggctta ccagtcgccg
    25381 cccagataaa ttatttgcga tgcgcgacct cggcgcgaaa ctcaggcgat ctttccgccg
    25441 agctcgtctt cgatgtggat ccggatgatc tcctcgaagc tggcatccgc cttgaatccc
    25501 agctcgctgg cgcggcgggc atcgaaattg cgcggccagc cggcgacgat ctgcatgatc
    25561 atcgggtctg gctcccgacg gatgcgggcc accaccttgt ccccggcgac cttccgcaga
    25621 gcctcgatct gttccccgac ggttaccgac agcccgggca tggtcagatt gcgcctcggg
    25681 ccgacctgct cgccagagat ggtcgcagca tgcagcagga aacccacggc ggcacgcggg
    25741 ctcgcgtgcc aatggcgcac gtcctccgag acgggcagca cggcttcctg cccggccaac
    25801 ggctcgcgaa tgatgttgga aaagaagcct gaggcggcct tgttcggttt tccaggacgg
    25861 acgcagatgg tcggcagccg aatcccgatg ccgtcgaaga aacccttgcg cgtatagtcc
    25921 gcgagcagca attccccgat ggccttctgc gtgccatagg acgtcagagg cgtatggaag
    25981 aactcgtccc cgatggcctc cgggaacggt gcgccgaaaa cggcgatgga gctggtgaag
    26041 acgacccgcg gcttgttgcc ggcgtggcgg atggcttcga acaggttgcg ggtgccatcc
    26101 agattgatgc gatagccctt gtcgaactcg agctcggcct ctcccgatac gatggcggcc
    26161 aggtggaaaa tcacgtccgg cttgtcggca acaagggctt ccgcctggcc tggctgggaa
    26221 aggtcgccaa ctttgacgga catcgggaac gggcaatccg ggcagcgcgc ctcgacgaca
    26281 tcatagagcg tgaggctgtc gatcggcttg tcaccgagct tgccgtcctt ggcgagccgc
    26341 tgggccagct tctgcccgac catgccggcg ccgccgatga tgagcacttt catgaatgtc
    26401 ccctttcaac ttgctagccc aagcgcacgc cgcagcgctc cgcccacttc aatccctcga
    26461 ccgtaccgcg cctcggcttg tactcgcagc cgatccagcc ttcgtatccg gtctcgtcga
    26521 tcaggcggaa gagccagcca tagttgagct ccccctcgtc cggctcgccg cgatcgggtg
    26581 gattggcgat ctggtaatga gaggtcagcg gggcgtactc gcggatcgcg gtggcgatgt
    26641 cgccttccat gacctgccgg tggtagatgt cgaactgcag gccaagattg ggctccccaa
    26701 cctcgtagat gatcgcacgc gcctcgccgg tgcgattgag aaagtagccc gggatgtcgc
    26761 gcgtattgat cggctcgatg agcacctcga cgccgaaatc ctgcgccatc cgcgctgcct
    26821 tcttcaggtt ggcgacgtag gtcgcgcgcg aggcgccgtg gtgcacgagc cccgccatgg
    26881 catggacgcg cgggcacttc agcgcttcgg cataggtgag cgcctggcgc atggctgccg
    26941 cgaacgcctc ctcacggccc ggcaaggcag caaggccacg ctcgcctttc gtccaatcgc
    27001 ccggcggcag gttgaacagc acctgcgtga gcccgttggc atcgaggcgc gccgcgatct
    27061 cgtcggcggg gtgctcgtag ggaaaaagga actcgacgcc cgtgaagccc gccttcgccg
    27121 ctgcctcgaa gcggtcgagg aaggcaagct cattgaacat cgtgctgaga ttggcggcaa
    27181 agcgcggcat cagccctgcc cttctgaaat atgtctgatc aggtcgatga tggaacggtt
    27241 actctgagct tccgtccggt cgatcgccaa gcgagttcgg gccgtaccat ccgataaggg
    27301 gccggcggcg cccactataa tggcgaaaga gccctgcagc gaggtgcgat tccgtctcgg
    27361 tcacaaggca aagccaccat cgaccagcat ggtcgtgcct gtcgtgaagg cgctctcgtc
    27421 ggaggcgagg tagacggcca tggctgccac ttcctctgcc gtcccgagcc ggcccattgg
    27481 ctggcggtcg atgaacatct gccgggcctt ctccacgctg ccgacggtct tggccagcgc
    27541 agcgatgcgc tcctcgagcg acggtgactg aatggtgccg gggcagatgg cattgcagcg
    27601 gatgccccga cggatgaagt cgacggcgac agacttggtc agcccgatga cggccgcctt
    27661 ggtggcccca tagacatagc ggttcggcac gccacggatg gagccagcgc cagaagcgat
    27721 attgatgatc gacccgccgc cattctcgag catgccgggg aggaacgcct tgatggtccg
    27781 gtgcatggag cggacattga gatcgaacgc aaagtcccac tccgcgtccg tacagtcgag
    27841 cactgttccg tgatggacga aaccggcggc gttgaagaga atgtcgacgg gcgagaaccg
    27901 ttttgccagc acctcgaccg acccggtgtc gcggacgtcg agctcgaggc attcggcgat
    27961 accctctttc tcgagccctt cgagcttgct gccgtcgata tcggtggcga tgacgcgcgc
    28021 cccctcgcgc gcaaatgcga tcgccgttgc ccggccgata cccgccccgg atgccgtgca
    28081 gaacgcaacc tttccctgaa gccgattcgc catcgctcac cttcccccga tttgcatgtg
    28141 gcatgagtgg acccgcctgg atttagcacg cagcgcgcgg gcagcctacc ggcgcaatgc
    28201 cggacgcctg ggcgattggc gcacatccgc tctctcatcc gagcatgacg gaccttaccc
    28261 accagccgcg atggccctcc gccagcgcgt cggcagcgga gcgagccgct tcgacactct
    28321 cgaaaagacc gaagacggtc ggccctgctc ccgacatgcg cacgaggcgg cagccgggat
    28381 aggcggccag ctccttctcg accgccttgc agacgggcat gaggtcctgc gccggctcgg
    28441 tgaggtcatt cgagaaggct tgaaggtagg tgatgaattc cgccggcgtg ccaaaggcag
    28501 gaggcggttc cggcgctcgc cctgtcgtct ccaacgcggg cgcatcaagg cgccggaaga
    28561 cctcccgtgt tttatcggcc ggaaccggat catcggcagt caccagaacg ccgggaagct
    28621 ggggtagatc gtgcaacaag cggagcttct cgccgacgcc ccacatgagc gccggagcct
    28681 gcgcaatgca gaccggcacg tcggcgccca ggcgagcggc cagcccgagc cagtcgatgg
    28741 tcccggcgaa acctggattg gcgcgccgca ccgcgcgtag cgttgccgcc gcatctgcgg
    28801 acccgccgcc cagacccgac gctacgggca aattcttctc tatcctgacc gctccgagcc
    28861 gcaagtccgg ctccgcggcg gccaagacgt ccagcgcctt cgccgccagg ttctcaccgg
    28921 cgaccttcga tgcaaacggg ccggtcatcg tcacggcggg ctctaaaccg acctcgagcg
    28981 tgaccaagtc gcccacgtcg gcaaaaacga ccaggctttc gagatcgtga tagccatccg
    29041 ggcgccgccc gaggacccgc aaggtcaggt tgatcttggc gcgggccagc tcgacgacag
    29101 aagttccggc ccgcttcgac cgcggtctgt cggccatgtt acctgccggg gttggctcgc
    29161 agcccccgcc acctattgga tcggcgcgcc aggggcgagc ttcgtctgtg cccgccgttt
    29221 gcctgcgtcc tcgcgcgcgg cctgccggct cttcttgggg ctaccggcca caggctcgtc
    29281 agtcaggcca tcctccagct tgcccttgat cttctcgagc tcttccggtt ccgggttgag
    29341 cgtcaacgcg tgctgccact gaaatcgtgc ctcgcgcacg cggcctacgc gccacaacgc
    29401 gtcacccaga tgatcgttca gcaccgggtc ctcgggcctc agctccactg cccgttccag
    29461 gaaacgcacc gcctcgtcgt aattgccgag ccggtaatgc gcccagccga ggctgtcgac
    29521 gatgtagccg tcgtcgggct tcagcgcgac ggccttctcg atgagcttga ggccctcgcg
    29581 caaattccgg ttctggtcga tccaggagta gccgagataa ttcagcacca gcggctgatc
    29641 cgggctgagc cgcatggctt tcttcagatc cgcctcggct tccggccact tcttgagccg
    29701 ctcataggac gtgccgcggg cataccaata ggtccagtgc cgctcctcgg gcttcttgat
    29761 gagcttgatc gcccgggtgt agtagttgac cgcctccgcg tagcgcttgc gcccacgcat
    29821 gatggtgccg atcgcatcga gcacacgcag atctttgggg ttcttctcga tcagcttgtc
    29881 cagcagcgct ttcgcctcgt cggtccggtc gagtgcattg aggttgatcg ccttgcggat
    29941 cgcgacagag gtgtcgagtg ccgtgccggt cggaatcttg tcgtagatgg cgatggcgtc
    30001 cgcgtaccgc ttcgtgctct cgtacacatt cgccagcgcc gccatagcga acgggaaatc
    30061 cggcttgagg tagagcgcca tctgcagata gagcgcgccg atgctgatgc cgccctcgcc
    30121 cgtcagcgcc tcacccagcc cgtagaagac ttccgccagc ccttcctcga ccgtactgac
    30181 gaggaacggc accttctcgc ctctttccag cctgtcgagc agatcccgtg acgtcggatg
    30241 accctcgccg ggcgtcttgc tgaggtgctg gtgcaggatg cgctttgcga gtttcaggtc
    30301 tccggccttg gccgcgtgct gggcataggc catggcggtg cgcggcgtgc gtgcgtccat
    30361 cttgaagacc agcgcaaacg ccttcccggc ctcggccgga cggcggccga tgtcggcgat
    30421 cagcccccgg tgatagcgga tgtagaactg cgcccattcg gcctgcttgc ccgcctccag
    30481 aagaccgagc gcaccatcga catccccgat ggcgagtttc acccaggccc gggccagcgt
    30541 cgatgtcagc tccccgatcg gcccggagcc ggccgcacgg aagtgctcct cggcgcccgc
    30601 aaaattgccc gacttgaagg cgctgagccc gagcacgagc tgggccattc ggtgggaatc
    30661 gtggcgctcg gcgatcgcct gggcgagctc gatcgcacgt ggccaattgc cctccgtcgc
    30721 ctccatcagg aaagtctgct cgatgagcac ctcgttgtcg ggatcacggg ccagggcgcg
    30781 cgaatagaac tccgccgccg ccgacgtatc gcgctgctcg cgcgcgtagc gcccggcgag
    30841 gtagctgccc aggagcgact cgacctccac cttcaggtca ggcttcgccg tcaccgctga
    30901 cagacgctgt gcggtggccg tcgcgaccgc cgtcgcagcg acggtgccca aacaagccat
    30961 ccacaggaaa agtcgtcgtg agcgcatagc acctctctga cggggcgcgg aggtgcccct
    31021 gatccgactc tgtgacgcac acattattat caaacactac acctctttgg cgactccgag
    31081 gcgggagaaa tcagcagagg actgattcga tttcgcatct gggttgaaga ggcgcgatct
    31141 ttgcatgcaa tcggctcacg ctgacggcgt tcggccgttc aatacttcga gaaatacgtc
    31201 ccgatcgata ttgccgccgg agaggatgac ggcggccttc ttgccgcgca gcctgtcgct
    31261 ctcgctgaga accgcggcga gcgccgcggc gcccgcccct tctgccacgt tgtgcgtctc
    31321 ggaaaagtaa atgcggatgg cttcggcgat ctcctcctcg ccgagctgga cgacgcgccc
    31381 cgcaccggcc cgtatcagct cgaccgcatc aggatgcggg tcccggcagg ccatgccgtc
    31441 ggcgaacgtc ctcgcggatg gcgtcgagac gaggtggcct gcatcgatcg acatcttcac
    31501 cgccggcgcc tgctcggcca cgacgccgac gacctccgtc ccgagcccga ggagatcgcg
    31561 caccgacatc accccgcaga tgccggagcc catgccgacg ggcacgtaaa cgacatccag
    31621 atcgccgacg gcctggaaca gctccagggc ataggtcgcc acgccgcgca cgagatgggg
    31681 atggaacgac ggcacgaaat gcagcccacg ctgttccgcg atctccgccg catagacccg
    31741 cgcctcgtcg aagtcgcgcc cgtgtacgat gagctcggcg ccgaaggcct ccatcgcggc
    31801 attcttctcg acgctgttgc cgtgtggaac gacgatgacc gcatcgagcc cggcgcgccg
    31861 tgccgccagc gcgatcgact gcccgtggtt gccgcgcgtc gctgaaatca cgccggtcac
    31921 gtcgggccgc gccgctttga gctcgtgtat gtaaacgagc ccgccccgga tcttgaaggc
    31981 acctgtcggg gtatggttct cgtgtttgac ccacagctcg cagccggcgc gtcgcgccag
    32041 gagcggccag gcatactggg gcgtgggcgg caccatctct cgaacgagcg ggagtgttga
    32101 ttccagctcc gacagcgtga acagggtcat ctcaccacac ctcccccggt cctgtagaca
    32161 ggcgaccggc gccgtcatgt agcctacatg ttcgaagaag gggatgcacg acgcctggac
    32221 cctgattcca gcaaatgagc accccttcgt gtcctttttc cgaaattggc tcacgcccgc
    32281 gaccgggttc gggaagcgga attcacaatg cgccgcgccc aatccgcgcc gttgcaggtg
    32341 gggctgcgct gcggatcggc aacgcaagag cggtttcgat gcggtcggct aacgcaatcg
    32401 atttctggcg cggtgtggcg ctgctgatga tcttcgtcaa ccatattccc ggaaacgctt
    32461 tcgggcagct cacactgcgc aactacgcga tctcggatgc cgccgagctg ttcgtcttcc
    32521 tggcaggctg gtcgttgtcc tacgccaccg gcggcccgca tcacccggag ccctatgccc
    32581 gtacgttttt ccgcctgatc tcgcgcgcga tcgagcttta ccgggcacag ctcgtcatca
    32641 cgatgctggc gctggccctt ctcgcatcgg tcgcgatcat tcggaacaat ccgctgtttc
    32701 tcgaatggca caacgccgcg tcggcgttct acgaccccat ccgcgcgacc atcgggatcg
    32761 tcctgctcac gcaccagctc ggatacttca acatccttcc gctctacatc gtgctgttgc
    32821 tttttgcgcc gctcatcgtc gtcctgacgc ggtggcgcgt gtggtcggct ttggcgctat
    32881 ccctggccgt ctacggcttc gccctggcca ccggcgtctc gctgccgaac tggcccgccg
    32941 aggagcggtg gttcttcaat ccgttgtcct ggcaggttct gctggtcctc gggtttgtgg
    33001 gggccgagct ctcccgttcc agcgagcggt tcaaccagat cgttgcacgc ctcgtgccat
    33061 tggggtggtt gctggccgca gtcggtgcct acctcgccct gaaccagctc tggccggacc
    33121 cgatgcgggt gccggagccg aagctcctgt tcctgttcga caagacctat ttgtccccgg
    33181 cccggatcat cagtctgctt gcgatcgccg tcgcgttcca aggggcgttc aagtatatcg
    33241 agcgcccgct cgggccgatt acgtcattga tgtgtgccta cgggcgcaac tcgttagctg
    33301 tcttctgtgt aggctcgctt ttgtcattga tcgggcaagt cgtgcgcgcg atactacccg
    33361 aaagtttcct cctggattgc gcaacggtac tttgtggcgt ctgcttaatg gggttcacgg
    33421 catggtttgt cgagtggaga agccgcatac ctggcttggt gtcgccgcga gcttcctgat
    33481 ctgcgcttcc gccgctgcac aggaacaggt ctccccgcag caaaggtttc aagtcgcgac
    33541 tctctcccgg gcagcaagcg agctatcgag cgattgcagc gtcaagaagg cttatgggcc
    33601 ccgggcgccg ctctacagcg tgcgcagggc tctgcgagag cagaggccgg tcaaagtcct
    33661 cgccatcggc tcgtcatcga cggtcggcgt cggggcgagc tcgccggcag cggcctaccc
    33721 ggcccggctc gaagtcaccc tcgagggatt cctgcccggc gtggacatcc ggatgatgaa
    33781 ccgcggcgtt tccggcgaga ccggccgcgg tgcggtggag cgcctcaagc tcgaggtcgc
    33841 tgaaacaatg cctgacctcg tcgtgtggca ggtcggaacc aacgacgccc tggcccggat
    33901 cgacgaggac gacttcgctg aaaccctccg cgaggcaatc gactggctgg aggccaaccg
    33961 gatcgacatc gttctcatcg acccgcagta cgtggagcgg ctggcggggg atggttacta
    34021 cacccggatc gtgaagacga tcgccgaggt ggccggcaag gagcgggtgc tgctcgtcaa
    34081 tcgattcgag gccatggccg atcttgcaaa gcgcaaggga aattcggcct tcctcgccca
    34141 ggatcgcttc cacctcaacg acgtcggcta tcgctgcatg gccgagtatg ccgcccgtgc
    34201 gatcgccgct ggcatcctcc aggccgaagc cgaggcggcg cggcagaact aacccaagcg
    34261 gcagtcgggc cgcttcgagc gactgcatga tcaggctggc gatggctgga tcgccggcag
    34321 aagccggctt tggacctggc tccccgatca tcgcggaacc cccaacttcc atccgggttt
    34381 tatccgaaga ccgatgacaa tggaggccac agtgatacac cccgtgctgg gggggttgtt
    34441 ggctgtcctc ggcgcattga gcattgccac gccggcatcc gcgaccgagc tggcgcagac
    34501 ctcagccaat gtggcgggat cggctccaga gttcagccag gccgcagccg atctcgagaa
    34561 gggtcagatg ttcatgacca ggggcctggt atgcgaccag ccgtccgaaa tcgatgcggt
    34621 cattactctt gcgaggaaag gcgagcctct ggaaggagcg ctgcagcaga tcaacaccgg
    34681 caccgaaacg ccgcgctgcg tcgtcggcaa gatgctgatc gcgcagtaca tgaagcaggc
    34741 ccgcaccttc ctggtcaacc agcagatgtt ccacgtccac caggtgcgag tcatcggcgt
    34801 tgccgtgccg acgccccgcg gcgtggtccc ggtgaagctc aagaagccca tggaacagta
    34861 cgtcgtctcg gccgacgaca ggaaggcggc ctgatcatgg ccgggcgcgc cggccgtttt
    34921 caggtgtccg tctccttgcc gagccaccac cgctccaaca agccgatgcc ctggagcacg
    34981 aggacggcca gtccggtggc cagcgcagcc agcaggtagt agcctattcc gcaggcgacg
    35041 ccgattgcgc cggcaagcca tatcgccgcc ccggtcgtca ctcccttcac gtcgccttga
    35101 gacttgatga tcgctccggc cgcaatgaac gcaacgcccg ccgtcaccgc atccacgatg
    35161 cgcgcagggt cggcaggtga gacctgggtc ccgcctcggt tgcccatctc gtagaaaatt
    35221 tcgatcgtaa tcagtgtgaa ggatgcggcc gccatggaga ccaaaatgtg tgtcctgagg
    35281 ccagcgggtc ggctacggag ctcccgctcg aaaccgattg ctgccccaag cacaatggca
    35341 agcagcatcc gcgcgaccat ggtcgtctcg ggaaggtggg agcgcaggat gtcgctcgac
    35401 acgcaggcgg acctccaact tccatcgcaa gccgaggaac gctcgcgtga ggttggatgt
    35461 tccgcttggc tgccggcaat tcacccgatc agcccgctcg agaacgcaga ttagcgcccc
    35521 gcgcctctcg cagtccgatg aacagcccgg ccgcaatgat gatcgcggag cccacccagg
    35581 tcccgaggtc cggaagtgtt cctgaaagca ggtagcccac gataaccgct ccgatcatct
    35641 gcacgtacat gaacggggca acgaggttcg ccggcgcata tgtcatcgac ttggccacac
    35701 accaatgccc caaaccgccg atcacgccga gcatcgccag caatccgacg tctgtccagg
    35761 ttggcggcgt cgtccacgcg aaagccgtga tgacggacat cacgacagtg ccgatcagta
    35821 cgctgtaaat cgccgacgtt tcagccctgt cgtactcggc cacgcggcgg gtaaagacct
    35881 gatcgaaggc ataggtcacc gcgctcgcca ggatcaggag agagccccaa tggaagactt
    35941 ccgttccggg gcgcaccacc acgagcaccc cgatgaacgc ggccaccacc gcggcgagac
    36001 ggtggccgct gatcctctcg cccagcatcg gccaggcgag cagcacgatg agaaacggtc
    36061 cggcaaagct gatcgacgcc gccttcgcca acggcacgta gaccagtccc gaaaagaaaa
    36121 gcatggtcgt ggccagcacc aacagcgagc gcatgagttg caggccgggg cggttgctca
    36181 ctacgatccg gaggccgtgg cgcggtgtga acaacgccag aacgaagatc agatgagaag
    36241 cggtgcgcgc ccagacgatc tgctcggagc tgtaacgatc gctcaggacc tgcacgaggc
    36301 cgttcatgac cggaaacaac atgtgggcgg cgcacatgaa gagaatgccg acgagaggtc
    36361 tcgatgtcag agcctggacg gcgatggtca tgcggaagga atctttctga aaccgagcgg
    36421 cggggcgcag cgcgctgctg cgtcccgcat gtcttgggct gcaggccagg caagttcaag
    36481 ttgaccagcc atgcgacgga agccgagctg gtctacgaaa cgtctttgtc agcttaaatg
    36541 ctccaagaaa taacagtatg cctgcccgcc ggaccggcta gaagagcctt gtggagatga
    36601 gccgtgcagc gtgcgcggaa ctgggaacgc atggaaaagt cggacgcgcc aggatccgag
    36661 cagcaacgga ccgatcgcct caaggcgatt gcgctgaccc tgctcgcggt gacgatgttc
    36721 gcctgtctcg acggaacggc gaaatatctc gtctcggtgg agctgctgcc cgtcgggcag
    36781 atcgtctgga tgcggttctt cgggcatttg ctgcttgttg cggtggtcct cggcttcgtc
    36841 agcatcccgc gcctgctgcg gacgcacaaa ctgaaatatc agctcgcccg ttcgttgctg
    36901 atgctctcgg cgacactgct caacgtgatc gcgttgcgct acctgcggct ggaccagacc
    36961 acgacgatcc tgttcctggc tccattgacc gtggccctgc tcgccggacc gaccctcggc
    37021 gaatgggtcg gctggcgcag gctcgtggcg atcctggtcg gcttcatggg catcctgatc
    37081 gccattcgac cgggcatttc cgccgtacac ccagcattcc tgctttgcct gggcggaatg
    37141 ctctcctact cactctatat cctcatgacg cgctatctcg ccgcccacga cacctcggag
    37201 gtgacgctgt tctattcgac ggtggtggga accattttcg ccgcgccctg ggcgttgtcc
    37261 gaatgggtct ggcccaccgg cccgctcggc tggatcctgc tgctttcgct cgggttctgg
    37321 ggtggcgcca gtcactacgt cttcattgtc gcccaccgct gggcgccgac ctccgcactg
    37381 gcgccctttc tctacggcca gctgctggct gccaccgcga tcggctattt cgttttcgat
    37441 cagcttcccg acgtctggac catcaccgga gcggggatca tcgtggcgtc ggggatctac
    37501 atgctgcaca gggagagaat cgtgaaacgt gaggatgtgg aagattaaga tcgtgaagtc
    37561 gtgaagtcgt gaagtcgcca agtcgtgaag ttgtggtcga cgaccgaaac caacctcact
    37621 atttcacgat ttcacgattt cactatcaca acgacttctg catctcgcgg acgaagtcgc
    37681 tcaggcccag cagccgctcg cgccgcagtc gctcggcggc aaggatagcc cgcaagcccg
    37741 cgacgctcgc ttccagatcc gcgttcacga tcacgtaatc gtattcggcc caatggctga
    37801 tctccgcagc cgcatcggcc atgcggcggg cgaccgaatc gggggcgtcc tgcgcccgcg
    37861 acctcagccg ctgctccaga acctcacccg agggtggcag gatgaacacg cgaaccaggt
    37921 cctgggcagc cttctcgttg agctgttggg cgccctgcca gtcgatgtcg aacagcacgt
    37981 cccggccgcc agccagtgcg cgctccaccg gcgagcgcgg cgtcccgtag tagttgtcga
    38041 acacacgagc ccattcgagc agctcgcccg catcgcgcat gcgccggaac tcggactcgc
    38101 tgacgaaata gtaatccctg ccctccacct cacccggccg cggcttccgg gtcgtcaccg
    38161 atacggacat ctcgatgccg ctgtccgcct cgagcaggcg gcgggcaagc gtcgtcttgc
    38221 cggccccgga cggcgaggac aggaccagca taattccccg gcgttcaatg gagggcgagt
    38281 gcacggcttt cactcgatgt tctgaatctg ctcccgcatc tgctcgatca gcgccttgag
    38341 atgcaacccc aaccgggtga tctgaccatc gttcgcctta gagcacagcg tattggcctc
    38401 gcggttgaac tcctgcgaca ggaaatcgag cctgcgcccg acaggctccc cggtatccag
    38461 caattcgcgg gcggccgcaa catgggcccg gagccgctgc agctcctcct cgatatcggc
    38521 cttggtggcc agcagcacgg cctcctgatg caggcgcgcc tcgtcgaatg cggaggacgc
    38581 gtcgagcaat cgctccacct gctcgcgcag ccgctgccgg atcgcctccg gagtgcgcgc
    38641 gggcgaagcg gcgatttcct ccacggctcg ctcgatcgaa agggtcaggt cgagcaatac
    38701 ctcacgcagc cgtgcccctt ccgaggcgcg cgcattttct accgcctgaa gcgcctcctc
    38761 caggcttgcc agcatgacaa gggcacgagc ctcggcttcc tcatcggtct cggaagcctc
    38821 gacgacctcc agcacgccct tcacgccgag cacgctgtcg accgaaacct gtccgccgcc
    38881 gacggccaca cgaacgcgct cggccgctgt caaaacctgc tgcagcagat catggttgag
    38941 ccggatctga gatacggcct cctcgcgctt aacggcgagg gcgattgcga cgttgccgcg
    39001 tttcaggcgc tttgcgacgg cttcccgcac gcgcggctcc agtgcctcga accccgccgg
    39061 caacttcagc cgtatatcga gtccgcggct gttgacgctg cgcacctccc agtgccagcg
    39121 catcggccct gcggcgccgt ccgcgcgccc gaagccggtc atgctcttga gtgtcatcgc
    39181 acaaaccacg agctggggcc ggcagcgcgg ccaaaaccag aagcgtcaga atcggcagga
    39241 gatcgagaag attcgatcca cgaattctcg ggccatcaca ctagcggctg gcttggccgc
    39301 aaacaaccgg acacggagcc gcgagggcca gcacaaggta gaactggatc tcgcgacgcg
    39361 tttggccggg ctgcctactg tcgcggtttg cgcaacggca gcggcggatc gccttcacta
    39421 gaggtgcctg cagcagccgc ggccgggttg acaagtcccg ggcccccgct ggccgccgca
    39481 ggaccgccgg ccgtcgtcaa ctgcttcgcg gcagcttcct tggcggcctc ggcctctctg
    39541 gcgcggatct cctgctccac cttccgccac ttggcgacgg cacggttgtg ttcgtccaac
    39601 gtctccgaga atacgctcga gccggtgccg ctcgcgacga aataaagata gttcgtgcga
    39661 gagggattga gcacggcgcg gagcgcctcg cgactcgggt tgcagatcgg cgtcggcggc
    39721 aggccgttga tctggtaggt gttgtggctg gtcttgcgct ggatttcggt gcgatagatc
    39781 ggacggcccc agtccacctg cccgccatcc aggccataga gaatggtcgg gtccgactgc
    39841 agtggcatct tccggcgcag gcgattgatg aagaccgacg cgatgtcctc gaacttgtcg
    39901 ttgcgcccgg tttcccgctg cacgatggat gcgaggatca ccgcctcgtc catgctttgc
    39961 agcggcagat caggctgacg ctccttccag gccgcctgca ggaacttctc ccgctcggcc
    40021 cgcatcatgt ccagcacggc ctgccgcgtc gtgccccggg gaaccagata tgtatctgga
    40081 agcaacgtgc cctcttccgg caccacgctg atttcgccgg tgaggttctg atcggcccgg
    40141 atgcgctcga caatctgatg gctggtcagc ccttcgggaa tggtcagccg gagcagcgcc
    40201 acctttccct cgccgatcag gtctgccacc tcccgcatgc tcatggccgg cttcagctca
    40261 tagttgccgg ccttgagttg aaggggcttg ccgccgaacc agcctgacat ctgccgcgcg
    40321 agataatagg cgacgaacac gtgctccctg gaaatgatgc cttcgcgctc cagacggcgc
    40381 gcgatatcgc gggcgccctc ccctctgcga atcaccagcg gtcgggcctc ctggagcggg
    40441 ccggccttgt cgagctcgga attgacccag aacacgccgc ccgcggcaac ggccaacgcc
    40501 aggaagctga acgtcaggaa cgcattgatg aagcggagtg tcgggcccaa ccttttcacc
    40561 tcacggcgcg tccgggaacc cttcggcctg gacggtgcac gtgtcggctc gagggcttca
    40621 gacggactgc gcggcatgac acccggaggt cgagttcgtc ctcccctgcc ggtccgttcg
    40681 ttcaccatca gctcgacacc cctccaccgt ccatcatgcg gtgacgaaag gcttaatcca
    40741 ggtcgtcaga cgcaaaatca ccggtgaacg ggtcgcgtca ccagcctcac gcaggatggg
    40801 cacggaatgt ggcaagagcg ggcaccggga tgcaacccct ttcggtgtgt tgcagaggtc
    40861 ccactcgagc gagacgaact atctcagccc acgaaccggc gcaggatgag cgaagcgttg
    40921 gttccgccga agccgaacga gttcgacagc acggtgtcga cctgcttctg cttggcggtg
    40981 tgcggcacga gatcgattgc ggtctcgacc gacggattgt cgagattcaa cgtcggcggc
    41041 accacattgt cgcgaatcgc gagcacaccg aaaatcgctt cgaccgcccc cgcggcgccg
    41101 agcaggtgtc cgatggcgga tttcgtcgat gacatcgaaa gcttggcggc cgcattgccg
    41161 aacagccgct cgacggcagc cagctcgatc tcatcgccca tgggcgtcga ggttccgtga
    41221 gcgttgatgt agtcgatatc gctcgggtcg atgccggcgc gcgcgatcgc cgccttcatg
    41281 cagcggtacg cgccgtcgcc gtcctcggct ggtgcggtga tgtgataggc gtcacccgac
    41341 agcccgtagc cgatgacctc ggcatagatg cgcgccccgc gcgccttggc gtgctccagc
    41401 tcctcgagga tcacggcacc ggctccctcg cccatcacga agccgtcgcg atccttgtcg
    41461 taggggcgcg aagcccgggt cggctcatca ttgaagttgg tcgacagggc ccggcaagct
    41521 gcaaagccgg caatggcaat gcggcagatc ggactttcgg tcccccctgc caccatcacg
    41581 tcggcatctc cgagcgcgat caggcgcgcg gcatcgccga tggcatgcgc acccgtcgag
    41641 cacgccgtga cgacggcatg attggggcct ttgagctggt gcttgatcga cacgtggccg
    41701 gaagcgagat tgatcaggcg cccgggaatg aagaacgggc tgacccgccg ggggcccttc
    41761 tccttgagca ggatcgacgt ctcggcaatg ccctgcaatc cgccaatacc ggagccgatg
    41821 agaaccccgg cgcgctcttg ttgctcgggt gttttcgcct cgaagcctga atccgccagc
    41881 gcctggtcgg cggcggcgac ggcatagatg atgaagtcgt cgaccttgcg ctgctccttg
    41941 ggctccatcc aatcgtttgg attgaaggtg ccgtccgagc catcaccacg cgggatgaag
    42001 catgcgatct ggcacgacag gtccgatacg tcgaactcct cgacccggcg cgcaccgctg
    42061 cgcccggcca gcagcctcga ccatgtcgcc tccacgccgc tggcgagcgg cgtaacgaga
    42121 cccaatcccg taataacgac ccgtcgcatg caaagtcctt ctccggcggc gcccggcaaa
    42181 ggcccgtcaa caccgcctcg cgtgatctgg ggagcagttc tgccgagcgg gcagcaccgc
    42241 caccggctta cgctgcgttc gcgttctttt cgaggaattt caccgcatcg cccacggtga
    42301 gaatgtgctc ggccgcgtcg tcaggaattt cgcagccgaa ctcttcctcg aacgccatga
    42361 ccagctccac ggtatcgagg ctgtcagcgc ccaggtcatc gatgaaggag gcgttctccg
    42421 tcaccttctc agcttctacg ccgagatgct cgaccacgat cttcttcacg cgctcggcaa
    42481 catcgctcat agctatttcc ctcgtgtcgt cagttgtcac atgagttgtc tggtggtcgc
    42541 cccggcccga aggacgcgga ccggaacgga gtctccgagc gggctaccag agtcacggtt
    42601 cgatgtaatc gaaatcgcct gatctcggca gcttttcccc aagtactcct tacgaaagca
    42661 ggcgctccta acacacttcc caggcgttta ctagcgttgc aatcgcccag gggacgcgca
    42721 ctccagatac gagcctagct ccttgtcatg taaacgcttt ttcgatcaga ccatggccat
    42781 gccgccgttc acgtgcagag tctgcccggt catatagcgg gcctcctcgc tggcgaggta
    42841 gagcgcggca gcggcgatgt cctcagggac gccgaaggtc tgcgcgggaa tatgctgcgc
    42901 aatcgcctcc acctgcttct cggtgagcgc atccgtcatg gccgtcttga tgaacccagg
    42961 cgcgatgcag ttggcggtga tgccgcgggc tgcgacctcg cgggcaagcg acttggtcat
    43021 gccgatcata ccagccttgg ccgcggcata attgccctgg cccgggttgc cgatgatccc
    43081 ggagatggac gtgatgttga tgattcggcc ccactggcgg cgcatcatgt tacgtaccgc
    43141 agcgcggcac agcatgaagg cggacgtcag gtcgacggcg atgacctggt cccattcctc
    43201 gtccttcatg cgcatgaaga gattgtcccg ggtcatgccc gcattgttca cgaggatgtc
    43261 gagggtgccg agcttcgcct ctgcctctcc agccagcttc tggacggcag cgcggtcgtc
    43321 gaggtcgcag ggcacgacat gcacccgctc cttaagctcg ccggcaagcg tctccaggcg
    43381 gtctattttg gtgccggaga tcgcaaccgt cgctcccgct gcgtgcagcg tacgcgctat
    43441 cgcactgccg atgcctcccg atgcaccggt gacgagcgca cccctaccag ccagactgaa
    43501 catgcccatt atctcccgag tgcagcgagc gcagcatcca catccgccgg cgtaccgacc
    43561 gacattgcct tggcatcctt gagaatgcgc ttcacgaggc ccgccagcac cttaccagcg
    43621 ccgatttcat agaagtcggt gacgccgagc gcgcccatct tctgcacgca ctcgcgccag
    43681 cgcacggttc ccgtcacctg ctcgacgagg cgcttcctga tctcatccgg atcgctgatc
    43741 ggctcggcaa gcacgttggc gacgaccggc acgacaggcc ggcggacctc caccttcgac
    43801 agctcctcgg ccatgacatc tgccgccggc tgcatcaaag aacaatggaa cggcgcgctc
    43861 accggcagcg gcagcgcccg gcgcacgccg tgcttgcggc cgatttcggc cacgcgatcg
    43921 atggccgatt tgtgcccgga gagcacgacc tgcgtcggct cgttgtcatt ggcgacctgg
    43981 cagacatcgc cctgggccgc ctcggccgcg accttctcgg cgacgtcgag accgacgccg
    44041 agcaaagcgg ccatggcccc ctgcccgacc ggcaccgcct gctgcatcgc ctttccgcgc
    44101 gcgcgaagca atcttgccgc atcggtgacg gacagcgctc cggccgccgc gagcgccgag
    44161 tactcgccga gagagtggcc ggcgacatag ctgacatgat cggcgaggct gacgcccttc
    44221 tcccgctcca gcacacgaac gaccgccatg gaaacggcca tcagcgccgg ctgggcgttc
    44281 tccgtcagcg tgaggacgtc cttggggcct tcccagatcg tgcgcgacaa cggctcgccg
    44341 agcgcgtcat cgacctcgtc gaagacgtcc cgcgccgcct tgtaggtctc cgcgagctcc
    44401 cggcccatgc caacctcctg gctgccctgg cccgggaaca cgaatgcaac cgccatctgc
    44461 cgctgccgcc ctggttatgc tggcgaatgg tgagtggcga atagcggata gggaaaccca
    44521 tccattcgcc attcgctatt cgccattcgc ttgttccctg cgctttaggc atggctcgga
    44581 cggcgctgtc aagccgatcg ggccgcaaag gacttgcggc tcgcgacggt tcgcgtataa
    44641 ggagcccgtt cgcaaagcca tcccggttgg gggctgaacg gcgggcacgc ttcttcgtcc
    44701 aacgatgaaa tgcgtggttg gggcgtgagc tccaggccgc cggtgtctcc gctcttcgag
    44761 gtcgtctttg gggcctttcg taccgcgcct tagcgcggtt tgaaccggcc ttgaaagggg
    44821 ctccgcgccg gggtggacga aatggatgag aggcaaatct gcatgccact ttacgagcat
    44881 gtcttcctgg cgcgccagga agtctcgcct cagcaggtcg aggcgatgat caatgaattc
    44941 gggcaggtga tcaaggacag cggcggtacc gtcggcaaga cggagtactg gggtctcaag
    45001 acgcttgcct acaagatcaa gaagagccgc aaggcccatt acgcgctcat gaacatcgat
    45061 gcctcgcctg aagcggtggc cgagatggag cggcgtatga gcctgtcgag cgacgttctg
    45121 cgcttcttga cgctgcgcgt cgaagagcac gagaccgagc catccgtgca gatgcgcaag
    45181 ggcgaggacc ggcgcgatga gcggggtgcg cgcggtgatc gcggcggggg ccggggcggc
    45241 gcccgcaccg agggcgcctc cacgttccgc gcaaggccgg ccggcgacgg gtcacggtcg
    45301 cgctcagagc aatccgggtc ggaggagtaa gtcatggcag acgtctctgt cgccggtggt
    45361 ccccgccggc ccttccatcg ccgccgcaag acctgcccat tctccgggca gaacgcgcca
    45421 aagatcgact acaaggacgt gcgcctgctt gggcggtata tttccgagcg cggcaagatc
    45481 gtgccgagcc gcatcacggc tgtctcggcc aagaagcagc gcgagctcgc ccgcgccatc
    45541 aagcgcgcgc gttttctcgg gcttttgcct tacgtcctga agtaatccga caggtggtcg
    45601 ccggcgggat tgccggcgat ctttcttgag cacacgtggt tgaggcggcc ggcaacgctt
    45661 gcgccccctc taaccgccca aagcgcggga cagcttgtgt catgccgttt gccctcctga
    45721 tcggagtcgg agccgggctc gtctcgggcc tgctgtttgc gtctgcctcg acggggaccc
    45781 tgctcgggct gatggtcctc gt
//
LOCUS       DASVDU010000180        51062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1677374, whole genome shotgun sequence.
ACCESSION   DASVDU010000180 DASVDU010000000
VERSION     DASVDU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 51062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51062
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677374"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VF226_11620"
     CDS             <1..592
                     /locus_tag="VF226_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074682.1"
                     /translation="KWEFRPEPGTHEVRVGLMGLGVMGEATAKALKALGFQLRGWSRS
                     ERNLEGVECFAGQEGLDAFLSGTDILVCTLPLTPDTRGILNRGLFSKLSQHGRHQRMP
                     GPVLINAGRGALQIEADILAALDAGELYAASLDVFETEPLPASSPLWSHPRVIVTPHN
                     AAESTPEAISHYMLRQIRRHQAGEPLENVVDRARGY"
     gene            708..1169
                     /locus_tag="VF226_11625"
     CDS             708..1169
                     /locus_tag="VF226_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074683.1"
                     /translation="MAPDDSFRKQLQHVITALETWAKEMAPWAQIELGPIGNAWRISA
                     VPHAETACPFEIVLRTDRQFDITVDGETYEDLPLDALTDIPQIVRAIANGRILIRRWQ
                     SSLTGLVYSVETVVDLPGADEGRFTRRNPDAPDVDDTELEAAIETFAPYRR"
     gene            complement(1180..2052)
                     /locus_tag="VF226_11630"
     CDS             complement(1180..2052)
                     /locus_tag="VF226_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7074684.1"
                     /translation="MPRLDPRLNAYREDLAAESLRGKVQAPRYVAGVKKQVIRAAVAV
                     RRRPVPTAGLDTEALFGEVVTVYDEANGWAWVQLDRDGYVGYVPANALTPEVRPATHK
                     VSALGTFLYPAPDIKTAPIMHLSINAPLAVAEHVDRFYRLAGGGFVIDRHASDKSRCA
                     RDFVEIAERLIGTPYLWGGRTRVGIDCSGLVQVSMEAAGLRAPRDTDMQQAQLGVALP
                     ITPGLEGLERGDLVFWPGHVGIMCDAFMMVHANAHHMAVTVEPLATAAQRIARSGTEI
                     SAIKRLPALSALEK"
     gene            complement(2067..3497)
                     /locus_tag="VF226_11635"
     CDS             complement(2067..3497)
                     /locus_tag="VF226_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEX7074685.1"
                     /translation="MLAENLIPPGLFLDQAKEARRIWLLGEGQELDVLPGLTDAQRTW
                     LRSMDFKGAARKHVLLPGEDGAIAGVVLGTGNGQNGDPCGPSALLVGQLASSLPPGGY
                     AFADEVEAAEVAAVAWGLGAYRFRRYKSNGGDEPPGLVLPAGADKAAVKHIVEGVWLG
                     RDLINTPASDLGPAEIEAAARSLAERHAASLTVVTGDDLAAGFPMIHAVGRASVRAPR
                     LIDLVWGAAEAPRVTLVGKGICFDTGGLDVKPASGMLLMKKDMGGAATALALAHMIMA
                     LKLPVRLRVLIPAAENSIGGNAFRPGDVLKSRSGKTVEIGNTDAEGRLVLADALTLAD
                     EEGPDRLISFATLTGAARVALGPDVPPLYTDDDAFAAELVSAGLSVGDPMWRMPFWNG
                     YERNLDSEVADMNNVSEGSFAGSVTAALFLRRFVRNTKAFAHLDIYGWRPSARPLGPK
                     GGEPHTARAVFEVLRRAYQPGTGQKS"
     gene            3792..5606
                     /locus_tag="VF226_11640"
     CDS             3792..5606
                     /locus_tag="VF226_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEX7074686.1"
                     /translation="MAGRNPFLPTLRATAVAVLLVLGSTANLFAEGAPATQTRLPQDV
                     ITEHVVELPGRTLRFTATAGSFPLTNSKGKVLAEMGYIAYTLDGMPADQRPVTFALNG
                     GPGSASAWLQIGALGPWRIRMEQAAASPSAPAPLLPNAETWLDFTDLVFIDPIGTGYS
                     HIVASDNADDGKDEQRRRTGRAGSRDEGGPSYFWSIEGDVESISEVIQKWIQKNGRSA
                     SPKLYVGESYGGFRGPKIARELQSEHGIALNALVLASPVLDFAGRRSTHPPISYVSLL
                     PSLAAAEMERHGEAITREALRDVEEYARTSYLEDLMRGPRDSAALDRIVAKVSEITGL
                     PEDVVRRHGGRISGRIYTEHVNRPAGKIASLYDASITGFDPDPMSGRSHYSDPFLTAL
                     KAPMTGAMLELYQSKLDYRTDRPYVKISGKVNNSWIWGNSPTPPESMSDLREALALDK
                     RFRVLVVHGYTDLVTPYFASELILDQLPAFGDRQRVTSVVYPGGHMFYSRDESRKAFR
                     EDAFNLVERIIVESNEAALLSGPPQTTGAAEPQEPAVPANPAEPAFPEGADEPPTAPN
                     AEAPPDQSSSRDTSDRRDPDVIVVKRGPAEGGRLYGAR"
     gene            complement(5529..7610)
                     /locus_tag="VF226_11645"
     CDS             complement(5529..7610)
                     /locus_tag="VF226_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7074687.1"
                     /translation="MDAKVPIGYSACPHDCPSTCALEVELLGNGRIGRVRGSKDNAYT
                     AGVICSKVSRYAERVHHPDRLLYPLRRKGPKGSREFERISWDDALDITAEAMLTAERK
                     YGPEAVWPYYYAGTMGLVMRDGINRLRHAKRYSEMYASICTTLAWTGYVAGTGKLAGP
                     DPREMAKSDLIVIWGTNPVNTQVNVMTHAIRARKTRGAKIVAIDIYHNGTMEQADLAL
                     CLRPGTDGALACAVMHVLFRDGYANWPYMERYTDCPHELEAHLRTRTPEWASAITGLT
                     VEEIETFARMIGTTPRTYLRLGYGFSRQRNGSVNMHAASCIASVTGSWLYEGGGAFHN
                     NGDIFHWDKSLIEGLDVADPSVRVLDMSRIGPVLTGDRYDLGDGPPVTALFIQNMNPV
                     QVAPEQRLVKQGFAREDLFVCVHEHFMTATAEVADIVLPATQFLEHDDVYQAGGQQSI
                     VLGPKLIEPPGECRSNHDVIVELAKRVGARHPGFEMTAREIIDWTLRKSGWGTLEELE
                     EKKWIDCQPDFESSHYINGFGYPDGKFRFRPKWPEVPVGRKHDWGIAHLPPELPDHWD
                     VNEKIDERHPFKLATSPARSYLNSSFNEMPSSRAREGRPRAKIHPDDLAELGITNGDR
                     IRMGNERGEILIHAEAFAGVQRRVVIVEGISPNAQFEGEKGINTLTSAIQPAPFGGAA
                     FHDNHVWIAPV"
     gene            7767..8057
                     /locus_tag="VF226_11650"
     CDS             7767..8057
                     /locus_tag="VF226_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074688.1"
                     /translation="MRLFLAALIALGGLAALPDHADARKKPRAPYAYRDYGPPPAYRN
                     YRRAPPPYGYRYRQTEDPNSSVCIEAEALDPGGNYAGYPCWARRAFAPKKEF"
     gene            complement(8068..8571)
                     /locus_tag="VF226_11655"
     CDS             complement(8068..8571)
                     /locus_tag="VF226_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7074689.1"
                     /translation="MISPAHVALMARYNRWQNQSIYGAADTLEDRQRREPRGAFFGSI
                     HATLNHLLWGDRMWMHRLAGWPAPAPKSIPESVSYFEDWNVLKAEREATDEKLVAWAH
                     ALQPEDFAGDLTWFSGAAGRNFTQPKWVLVAHLFNHQTHHRGQVHCLLTQLGAKPQPT
                     DLPFMPE"
     gene            complement(8670..9092)
                     /gene="ndk"
                     /locus_tag="VF226_11660"
     CDS             complement(8670..9092)
                     /gene="ndk"
                     /locus_tag="VF226_11660"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7074690.1"
                     /translation="MAIERTFSIIKPDATERNLTGAINALIEKAGLRIVAQKRVRWTR
                     AQAEKFYEEHKARPFYGELCDFMTSGPIVIQVLEGENAIARYREVMGATDPAKADEGT
                     IRKMFGRSVGENSVHGSDSPASAEREILLNFGLNEIVG"
     gene            9505..10761
                     /locus_tag="VF226_11665"
     CDS             9505..10761
                     /locus_tag="VF226_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEX7074691.1"
                     /translation="MAGAASQIEWYIARDGQQYGPLSDLEMRKFVELGHLRPSDLVWR
                     YGFPDWRPAPAVFPTAAPPRPEPSRPSYRPEPRPQPQQPRYEPARPSSREANEHGAAS
                     ARTAAAPVAVSNPYEDDDDDDGDDFLPEQPRKGWRRVAVAAILVALLGGAIWLAVDLV
                     PSPANLIALIEAPKTPSGGPPDEAAGTESSPSQTLQSARADAAVPDGLFLNTPLWQLI
                     KREFPEWYEQRVKEAAQLEAEGRDPEFIQRRLTENVVALRRQHAEEALAASPGTLRGI
                     AQNFLENLQRLSAESVEACYGFISKGEASPQVLALMNSPEHRERLQRQVTAVFEAVAE
                     GRRSPQTHLPPRQADYQALTQALAGRGWTNNDIQLFSNPRALAQAPPQEVCRLVQDWF
                     AAQLSIPDEDVQLRLLVESLKPVVAG"
     gene            10895..12805
                     /locus_tag="VF226_11670"
     CDS             10895..12805
                     /locus_tag="VF226_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7074692.1"
                     /translation="MLHINDLTFRIEGKIIFEGATAGIPAGHKVGLVGRNGAGKTTLL
                     RLITEELAPDGGSISYPKHTRIGYVAQEAPGGDETLIDWVLSADRERTELLAEAETAT
                     DPHRIADIHQRLADIDAHAAPARAAQILAGLGFDEAAQRRPCREYSGGWRMRVALAAV
                     LFLEPELLLLDEPTNYLDLEGTMWLESYLAAYPHTVLIVSHDRDLLNRVVGSILHLDR
                     GKLTLYTGNYDEFEDLRRERLSQQLALKKRQDEERRRIETFIARFKAKASKAAQAQSR
                     VKALARMKPIVAQVEERVVPFNFPKAEKPLASPLLRLEDVAVGYEPDKPVLSRLNLRI
                     DNDDRIALLGQNGNGKSTFAKLLVGKLDPMAGRVIKAHRLEVGYFAQHQLDELVPSKT
                     PYDYITELMPDATEAQRRTRLGGYGFGIEKADTRCSNLSGGEKARLLFMLTAFHGPHL
                     LILDEPTNHLDVDSREALMQALNEFEGAVILISHDRHLVEASADRLWIVRDGTVTPFD
                     GDMDDYRQLLLAERSARNRERAKDGAGSEPRVGRAEQRRAAAERRAELAPLKKAMQQA
                     EKEVEKITAEIARIDAALADTDLYARDPDKAQRLSIDRGLAAKRLADAEEAWLAAIEA
                     YEAAASEADAVA"
     gene            complement(12943..13185)
                     /locus_tag="VF226_11675"
     CDS             complement(12943..13185)
                     /locus_tag="VF226_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX7074693.1"
                     /translation="MAEEPIVAQKGPYQVELQEGRVYLWCACGRSQKQPFCDGSHKDT
                     GIEPVRFVAETSGIVNLCGCKATDDQPFCDGSHLLL"
     gene            13355..15013
                     /locus_tag="VF226_11680"
     CDS             13355..15013
                     /locus_tag="VF226_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7074694.1"
                     /translation="MRHGGKILIDQLEAQGVTTAFTVPGESFLAALDGLHDSNQIRTI
                     ICRQEGGAAMMAEAWGKATGKPGICFVTRGPGAANAMSGLHVAQQDSTPMILFVGLPG
                     ERHEDREAFQEIETKQLFSSFVKWAAVIRATERIPEYVSRAFHVASSGRPGPVVLGLP
                     EDMLSAKADAKDARAARITQPAPSATTMAEFADLLAKAERPLMIVGGPGWSTDVQQRI
                     ERFVERFDLPVAAAFRFQDYIDNRHPCYVGHVGIGIDAKLAAAVRDADLLIALGARLG
                     EMTTSGYTLLDIPNPSQRLVHVHPSPDELGTVYAPDLPITTTAEQFAAALGGLTPPAN
                     IAWKQRRADLRAACEAAWTPIATPGAVQLEQVIRTVSDMLPEDGIVTNGAGNYAAFVH
                     RYFQYKGYRTQLSPTSGSMGYGLPSAIAAKLANPTRPVVSFAGDGCFMMTSQELATAV
                     QYGTNVVTIVCNNGMYGTIRMHQERKYPNRVVGTTLVNPDFAAFARAFGAHGETVRHT
                     EEFRPAFERALAANKPAVIELKIDAEALTPRQSLSQIRASGAKS"
     gene            complement(15551..16477)
                     /locus_tag="VF226_11685"
     CDS             complement(15551..16477)
                     /locus_tag="VF226_11685"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7074695.1"
                     /translation="MNVPRNFNGVIAPVLTPFGDDGAPDPDRFIEHAEWLMEDGCTAL
                     APFGTTSEANSIGLDERMELLEELVDSGMDPSKLMPGTGACSVADAAMLTQHAVDLGC
                     GGVLMLPPFYYKNPSEEGLFRFFADVIDEVGDDRLRVYLYHIPPVAQVGFSLSLIERL
                     KNEFPGVVVGLKDSSGDWSNMKAILDAFPDFELFPGSELFLLDGLRNGAAGVISATAN
                     ISARAMRQLYDNWRGPNADAMQEKISALRKTVQSFPVIPTLKALIAHYRQDEGWAEVL
                     PPFTPLPAEEAQRAIKILADQHNFRLDFAEAA"
     gene            complement(16538..17167)
                     /locus_tag="VF226_11690"
     CDS             complement(16538..17167)
                     /locus_tag="VF226_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7074696.1"
                     /translation="MIDLYTWSTPNGRKVSIMLEEIGLPYTVHPVDIGKNQQFEPAFL
                     KISPNNRIPAIVDRDNGMSLFESGAILMYLAEKTGKLWPKQGEAHWRVVEWLMWQMGG
                     VGPMLGQVHHFVKFNRGKAPYAEERYTKEALRLYGVLDRRLGEVEFLAGDYSIADIAT
                     WPWISRFEWQEIALDKFPNVLRWYRAIAERPAVQKGYHVPVPQPSIPMP"
     gene            complement(17411..18328)
                     /locus_tag="VF226_11695"
     CDS             complement(17411..18328)
                     /locus_tag="VF226_11695"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7074697.1"
                     /translation="MSLFIEILTQVTLPIITLVALGWLLQPRLKLDVGSLNRLQVYVV
                     MPAFLVHFLSTGNQPLSVIWPVVYFSVVQFIILIPLGWLLVHLLRMRPSLGPMMGLGT
                     AYANVGFFGIPVTQLAFGADYLIYQSVLTALMAILVCTVGVWLLAPAGGSRLVKLRNA
                     FETPLIPSVILGIALRGLEVELPALVGQPLQFLGSIFTPLALYTLGAQVAAARAVNFE
                     ALPQTLMLLLKFVAAPALSWWICHAMGLPPDITAVMVVAAATPVGVLITIFAAEYKTE
                     SEFISTAVVLSTALSPLFVTAWILATRLT"
     gene            complement(18433..19134)
                     /locus_tag="VF226_11700"
     CDS             complement(18433..19134)
                     /locus_tag="VF226_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074698.1"
                     /translation="MARRAQPKQHFTRDRMAGDSGCLVVVTAAEQAQLDLLLTAARRR
                     IPHSTLEFPKRITTRRDGPPDAEIAVTRSLFRQIEQDGGFMATWGSEGHRFGLLDSIQ
                     RMLISGRSAVIAAPLNLVGELYDVCPDVRALRLTGQLDAVRATLTPKACLRRIVGPRL
                     AERLESRGAARTIAVPHGGDMPSAVRALSEALARIEQEAGLIAPSTAGSSTSRRSRAG
                     SPARSKSPHAAPAAP"
     gene            19405..20082
                     /locus_tag="VF226_11705"
     CDS             19405..20082
                     /locus_tag="VF226_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7074699.1"
                     /translation="MTIASGAIIHSLLDSFAAAEVRDFPYRHWLLTRCFPEEAVDEVL
                     ALPFPAPALGGISGKREIHNATRKYFDVENRGRFPIVASVAEALQDRRVTSAVERVFG
                     VRLGGSYLRIEYAQDTDGFWLEPHTDLGVKLFTFLIYLSKDPSHADLGTDIYDLEKRH
                     VGRSPFVSNGAMVFVPSDITYHGFEPRKINGVRKSIIVNYVTGAWRAREQLAFPDNPI
                     ASMAAAA"
     gene            complement(20092..21591)
                     /locus_tag="VF226_11710"
     CDS             complement(20092..21591)
                     /locus_tag="VF226_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7074700.1"
                     /translation="MDVFGNLAHGFAVALTPLNLMWSLVGVTLGTAIGILPGIGPALT
                     IALLLPVTYHLDPTAAFIMFGGLYYGAMYGSSTTAILINTPGETGSLVTTIDGNKMAR
                     AGRGAAALATSAIGSFVAGVIGTLLLALLAPEVVKLALLFGPADYFALMVVAFAAVSS
                     LLGSSVLRGLVSLFIGITIGLVGIDQQTGQSRFTFGVLELQDGLGVVLVAVALFAVGE
                     ALFLASRADLRREEIVPVKGRAFMTREDWARSWKPWLRGTLIGFPFGALPAGGTELPT
                     FISYKVEKSLAKRPEEFGQGAIEGVAGPEAANNAAVAGVLVPLLTFGLPTSSTAAIML
                     AAFQQYGLQPGPLLFSSNSALVWGLIASLLIGNAMLLVLNLPLVGLWVRLLYIPQPWL
                     YGGISVFAMLGVYGINRSLVDVMALIAIGLLGCLMRRLDYPIAPVIVGLILGPLSEIQ
                     FRRAVQISQGDLSVFVTRPISAGLLVLAALMLAGAIAAHWRGSRQAATA"
     gene            complement(21595..22086)
                     /locus_tag="VF226_11715"
     CDS             complement(21595..22086)
                     /locus_tag="VF226_11715"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7074701.1"
                     /translation="MTGRFNAHALVGLALVLIGAGLFISAQEIHVSPAVVLLGPRLFP
                     SIVAVALVVLGVLTIASALFGSHDGPEAGSENDTPENDWPAFLTALAGPVLFLLTVEA
                     LGFAVATALLFAAVARAFGSRRLIADLGVGLLMGLVVLLVFSYGLGLLLPVGSVFDSL
                     LGG"
     gene            complement(22148..23113)
                     /locus_tag="VF226_11720"
     CDS             complement(22148..23113)
                     /locus_tag="VF226_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7074702.1"
                     /translation="MGKGTTRRTFFAIALAAAMALPASAQQMDQLTIMAPAAPGGGWD
                     GTARVMQDVLQSAGIVKSVTVENVAGAGGTVGIAQFAKKKGDGKSLMVSGLVMVGAIL
                     SNKSPVTLTDVTPIARLTSEWEAIAVPADSELKTLGDLIAKLKADPGSVSWGGGSAGG
                     TDHITAALFAKAVGVDPAKVNYIAHSGGGEAMASILGGHVTLGINSASELEPQVQAGK
                     LRWLGVSSDQRIAGIDAPTFKEQGVDLVIGNWRAVMAPPGISQADREKLTAVIDAMVK
                     SDQWKQKLTERNWLDNYLSGPEFERYLAEENQRVTEILKSVGLVN"
     gene            complement(23313..23690)
                     /gene="secG"
                     /locus_tag="VF226_11725"
     CDS             complement(23313..23690)
                     /gene="secG"
                     /locus_tag="VF226_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7074703.1"
                     /translation="MVTVILLIHIMIAAALVGVVLLQRSEGGALGIGGGGGGGFLTGR
                     GTANLLTRITAGLAAAFFLTSTILSLMAQRASAPPSIFDQPAAQQQGTEAPAGENQQD
                     TTPRGGILDRLQPPAGPSVPQGR"
     gene            complement(23791..24168)
                     /locus_tag="VF226_11730"
     CDS             complement(23791..24168)
                     /locus_tag="VF226_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074704.1"
                     /translation="MHLRCALYSLLPLAAAAALLPADPASAVPLDPQTCIQLREERLN
                     LVMDGARDNMARGPEWGRANLTPAQLKAVQRLIELDEQIGFRCPDVVATASEPSNVVP
                     LPKRKPKTHAAAPAPKRKPRAGR"
     gene            complement(24208..24993)
                     /gene="tpiA"
                     /locus_tag="VF226_11735"
     CDS             complement(24208..24993)
                     /gene="tpiA"
                     /locus_tag="VF226_11735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7074705.1"
                     /translation="MTASTAYQAPIRPLIAGNWKMNGLKADLAEAAAVRDAIVSGQVS
                     HGIDVMICPPATLISALAELADGSRLAVGAQDCHWLPSGAHTGDISAEMLHDAGATAV
                     IVGHSERRADHGELDRIVKAKAEAALRAGISAIICIGETAGQRRAGLTLDVVARQLSG
                     SVPEASTAADTIIAYEPVWAIGTGLTPTVDEVAEVHAFIRTRLSDLLGQQGREMRVLY
                     GGSVKPSNAAELLAVPHVNGALVGGASLKAKDLLGIVAACKVG"
     gene            25196..27094
                     /locus_tag="VF226_11740"
     CDS             25196..27094
                     /locus_tag="VF226_11740"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7074706.1"
                     /translation="MLDALRRGATSWVAKIFLGLLVLSFAVWGVADVFRGYNEGALAE
                     VGDRQITSDEFQRALQLELAMLGRQIGRRPTMEQARAFGIDTRVLSRLIGAAALDSHA
                     EELRLSITDEAVAEEIRNDPSFKNPDGSFNRMALESVARELGLSEYGFLALRKKEEIR
                     QQLTDAITAGAYVPDTLVKALYDYRNEARNVEHFTIDPNVAIKLPEPDPAKLKELYES
                     EKQRFMTPEYRKLAVIGLTMEEMKKRVPITDAELSAAYEQDKARYEVPERRRVLQIAF
                     KTPEEAEQAAAAIRGGKSFEDVAKEAGIVESDYTLGLLSRDELIDPAVAEAAFSLENG
                     AVSDVVRGRFAPVLVKVTEIQPGKQRTLDEVKDQVRDTLAADKAGPELNRLHDEIDDR
                     RFAGKPLKEIAQELDLPFLEIEATDKTGKAPDGSVVVKGPDADRILKAAFEGQVGFDG
                     DSIELADGGYGWVNVISVTEAKQRTFEEAEADVAALWRERETRRMLSELGSQLAERAG
                     KGESMEALAQEVGGKVETARNVKRIGGAPGLPDGAVGQAFITARDAAGSAETRDGKSR
                     VVFKVTEVIPPPAATKEELDRLRSELERQVEGDLLSGYLGGLQDRLGVSINQAAYLRA
                     VGASPEQQ"
     gene            27107..28684
                     /gene="trpE"
                     /locus_tag="VF226_11745"
     CDS             27107..28684
                     /gene="trpE"
                     /locus_tag="VF226_11745"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7074707.1"
                     /translation="MSKPAIPGLEVSPGYTSFAQKYEKGESQVVSTRLVADLETPVSA
                     YLKLTGAPGRESRMSFLLESVEGGAVRGRYSIIGLEPDLVWRAFGETAEVNNNPIGEP
                     DAFERERQPTLAALRALLARSAIALPEDLPPMAAGVFGYMGYDTVRLIEHLPNPRPDA
                     LGVPDAILMRPTLILIFDAVKDEMVLVTPVRPAKGVTARAAFEAAEARLLRAASALET
                     PLPHSAAGQGALELPEPESNTSPAEYMSMVARAKEYIAAGDVFQVVLSQRFSMPFPLP
                     PFALYRALRRTNPSPFLYHLDLGSFALVGSSPEILVRVRRGEVTIRPIAGTARRGATA
                     AEDQALAEALLADPKERAEHLMLLDLGRNDVGRVAEIGSVEVTERFVIERYSHVMHIV
                     SNVVGRLDPKKDAIDALMAGFPAGTVSGAPKVRAMEIIDELEKDKRGPYAGCVGYFSA
                     DGQMDTCIVLRTAIVKDGVMHVQAGAGLVYDSIPENEQQECINKAKALVRAAEEAVRL
                     ANRSPRSTERGNGGPFA"
     gene            complement(28879..29157)
                     /locus_tag="VF226_11750"
     CDS             complement(28879..29157)
                     /locus_tag="VF226_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7074708.1"
                     /translation="MSKKDLIDAIARHAELSKEKAGAAVDAMVSYIESTLSKGEEVSI
                     PPLGKFKVSKRAARTGRNPSTGQEIKIPAANVPKFQPSKTLRDSINRG"
     gene            complement(29400..31820)
                     /gene="lon"
                     /locus_tag="VF226_11755"
     CDS             complement(29400..31820)
                     /gene="lon"
                     /locus_tag="VF226_11755"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7074709.1"
                     /translation="MSEVKATKDKGAGKELFPVLPLRDIVVFPYMIVPLFVGREKSIN
                     ALEEVMRSDKHILLAAQKNAGDDDPSTDAIYGMGTLASVLQLLKLPDGTVKVLVEGTT
                     RARICRYTDNPDFFEAEVERVTETIGAKDEIEALARSAVSQFESYVKLNKKISPEVLG
                     TVSQIEDYSKLADTIASHLAIKIADKQEILETTVISDRLERVYALMESEISVLQVEKR
                     IRSRVKRQMEKTQREYYLNEQMKAIQKELGDTDERDDIAEFEEKIENTKLSKEAKDKA
                     LAELKKLKQMSPMSAEATVVRNYLDWLLSIPWGVRGKVKKDLAEAQEILDKEHYGLEK
                     VKERIIEYLAVQNRTNKLKGPILCLVGPPGVGKTSLGKSIANATGREFVRMSLGGVRD
                     EAEIRGHRRTYIGSMPGKVIQSMRKAKRVNPLFLLDEIDKMGSDFRGDPAAALLEVLD
                     PEQNSTFNDHYLEVDYDLSNVMFVTTANTLNIPPALMDRMEIIRIAGYTEDEKIEIAK
                     RHLIPGQKQAHGLEEGEFSISDEALKLIIRRYTREAGVRNLEREIAKLARKAVKEILT
                     SEKKSVEVTPENIETYLGVPKFRYGEAELEDQVGVVTGLAWTEVGGEILTIEGVMMPG
                     KGKMSVTGNLRDVMKESIQAANAYVRSRSVDFGIPPTLFERRDIHVHVPEGATPKDGP
                     SAGVAMVTAIVSVLTGIAVRKDVAMTGEITLRGRVLPIGGLKEKLLAALRAGIKKVLI
                     PEENVKDLADIPESIKNALEIIPVARMDEVLKVALVRQPEPIAWEEPAAAVSGGETQP
                     GSSRITAH"
     gene            complement(32115..33368)
                     /gene="clpX"
                     /locus_tag="VF226_11760"
     CDS             complement(32115..33368)
                     /gene="clpX"
                     /locus_tag="VF226_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX7074710.1"
                     /translation="MAQQEKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELCMDII
                     REENKNTLVKSRDGVPTPQEIKTVLDSYVIGQDHAKRVLSVAVHNHYKRLNHAAKNND
                     VELAKSNILLIGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENIILKL
                     LQSADYNVERAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGTIASVP
                     PQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIISRRGKGTSIGFGARVIAADERRT
                     GEVLRDVEPEDLLKFGLIPEFIGRLPVIATLEDLDEDALVQILSEPKNALVKQYQRLF
                     EMEDVKLTITQEALRAIARKAIERKTGARGLRSIMEAILLETMFELPSMKGVEEIVIG
                     PEVVEGGARPLRIYTERAEEKGSSA"
     gene            complement(33733..34377)
                     /locus_tag="VF226_11765"
     CDS             complement(33733..34377)
                     /locus_tag="VF226_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX7074711.1"
                     /translation="MRDPIDTYTNTFWPMVVEQTTRGERGYDLPSRLLKERIIFVTGP
                     IEDQSAASICMQLLFLEAENPKKEISMYINSPGGVVTSGMAIYDTMQFIRPPVATLCI
                     GQAASMGSLLLCAGEKGMRYALTNARVMVHQPSGGFQGQASDIERHAEDIIKMKRRLN
                     EIYVKHTGQPYETIERTLDRDYFMTAEQARDFGIVDRVLLKREEFEAAAEAKAT"
     gene            complement(34585..35934)
                     /gene="tig"
                     /locus_tag="VF226_11770"
     CDS             complement(34585..35934)
                     /gene="tig"
                     /locus_tag="VF226_11770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7074712.1"
                     /translation="MQVTETLSDGLKRQLRVVVDAGEIGAKFDARLDEIKDRVQLKGF
                     RKGKVPVQHLKKVFGRSLMGEVLQQTIEEKSRAALDERKERPAIQPQIDLPEDQQEIE
                     KVMSGQADLSFGMSFEVLPEINLIDFSELQLEKLTADVDDSAVEQAINQLVERSTSYE
                     VTEGRVAEDGDRLTIDFVGKIGGEPFEGGAGEGVNIVLGQGGFIPGFEDGLKGAKAGE
                     ERTVTATFPENYPEKSLAGKEATFDVKVKEVAVPKKPEVNDEFAKNLGVENLEKLREL
                     VTEQIKREYAQASRAKLKRALLDELEKRHDFALPPTLVEREFEAIWRQVTQGLEQAGR
                     SFEDEGKTEEQAREEYRKIAERRVRLGLILGEVGEKSGIQVTQDEMRRALMEQARQYP
                     GQERLVYEFYEKNPGAIAELRAPIFEEKVVDHILGQVKLEEKKVDREELFKPEPDEE"
     gene            complement(36037..36121)
                     /locus_tag="VF226_11775"
     tRNA            complement(36037..36121)
                     /locus_tag="VF226_11775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36085..36087),aa:Leu,seq:tag)
     gene            36364..37029
                     /locus_tag="VF226_11780"
     CDS             36364..37029
                     /locus_tag="VF226_11780"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HEX7074713.1"
                     /translation="MLALLGTRRIATLCACVVTLLSAASLAATDRPADTRDHVGIVHR
                     ARGQVQIERNGVLIKDVTAGHTLARRDQLSTGPGSRVILSFNDGSQLAVGENAQLVIA
                     DFMREEGRRSGALILDLIRGAIRLVAAKPKEAPTKRVEVRTTAATISSRGVDVWSSCI
                     GDKLAVLVLKGKVDVRNDAGLVILDRKRLGTFVADRLSAPEKPSVWPKARAQESLLAV
                     AFR"
     gene            complement(37052..37192)
                     /locus_tag="VF226_11785"
     CDS             complement(37052..37192)
                     /locus_tag="VF226_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074714.1"
                     /translation="MFRLPLLVAGVLCAYQFWPYIQADERLMWAALLPAVYFVGMWAL
                     DS"
     gene            37425..38474
                     /locus_tag="VF226_11790"
     CDS             37425..38474
                     /locus_tag="VF226_11790"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7074715.1"
                     /translation="MTVASSVADAVGHTPLIRLRRASEETGCTILGKAEFMNPGQSVK
                     DRAALYIIRDAVARGALRPGGTIVEGTAGNTGIGLTLIGAAMGFRSVIVIPETQSQEK
                     KDALRLLGATLVEVPAVPYRNPNNYVKYSARLAEALAKTEPNGAIWANQFDNVTNRQA
                     HIETTAEEIWEQTGGKVDGFACAVGSGGTLGGVSIGLKAKRKDIQIGLADPPGAALFS
                     YYTTGELKAEGSSITEGIGQGRITRNLEDVEVDHAWLIEDAEAVSIVFQLLQEEGLCV
                     GPSSGVNVAGAIRLARKMGPGHTIVTILADYGTRYQSKLFDPSFLRSKNLPVPSWLEN
                     QRQDIPSVFVDPEQT"
     gene            38493..39221
                     /locus_tag="VF226_11795"
     CDS             38493..39221
                     /locus_tag="VF226_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX7074716.1"
                     /translation="MPATEPLYRADPYLRDCTATVLAINDRGGIILDRTIFYPTAGGQ
                     PGDRGGLEIVGSGSCPIATTVYDEDRTTIVHVPAEGAMAPQPGSSVRLVLDWETRHRH
                     MRMHTALHLLCALVKFPVTGGQIGADESRLDFDIADASAVDKDQLTEAINQLIAADHP
                     VSERWISDEELDANPQLVRTMSVKPPRGSGRVRLVAIGENGSVDLQPCGGTHVRSTGE
                     IGRIVVSRIEKKGKQNRRIRLAFA"
     gene            39254..40111
                     /gene="sseA"
                     /locus_tag="VF226_11800"
     CDS             39254..40111
                     /gene="sseA"
                     /locus_tag="VF226_11800"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEX7074717.1"
                     /translation="MAPVSGSYVVETDWLAAHLNAPDLVVLDGSWHLPTAGRDACAEY
                     LAEHIPGALFFDIDEISDETSPLPHMLPSTVKFASRMKKMGIGDGMRVVVYDSVGMFS
                     AARVWWTFRVMGHEDVAVLNGGLPKWKAEGRPVTSEPPPARSERHFTPRLNASLVRDL
                     EEMKRIVESGSAQIADARGPGRFTGTEPEPRPGLRSGHIPGSRNVPYSRLCNPDGTLK
                     PAPELHRLFSEAGIDPSKPVVTTCGSGVSAAVLSLALAIIGQPDSALYDGSWSEWGRD
                     DAGTPVETG"
     gene            complement(40156..41139)
                     /locus_tag="VF226_11805"
     CDS             complement(40156..41139)
                     /locus_tag="VF226_11805"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX7074718.1"
                     /translation="MLLGIAAVAAAAVYLNNSSYLAQPPADRPSLLAHRGLAQTYDKT
                     GLTGETCTATRIDPPVHDYLENTIASMRAAFELGADVVELDIHPTTDGHFAVFHDWTL
                     DCRTNGHGVTREHSLAQLKALDVGYGYSADRGKTFPFRGKGVGLMPSLDEVLAAFPDK
                     PLLINVKSNDPGEGRLLAARLSVLPSERLEQLMVYGGDRPIAVLRQELPALRVMSRRT
                     LQDCFIRYALTGWAGYVPEACRGTLLLVPTNIGPWLWGWPHVFQRRLERYGTKIYAVG
                     PYRGGEFSTGVNTREELQSLPDGYAGGIWTDRIDVISPLVLQHRPDASHDD"
     gene            complement(41253..42875)
                     /locus_tag="VF226_11810"
     CDS             complement(41253..42875)
                     /locus_tag="VF226_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HEX7074719.1"
                     /translation="MNSKTGKITYVSRRQVLSGAGGLVSVALLARQAWAEGASGLIVG
                     TGFPFSYEWLKERARTLAKGPYVPPPRPAPEIVQQIDYEAHGAIRFKPDYALFANGPG
                     RFPVTFFHLGQNFPKTVRMHVVIAGEAKEIIYRPEYFDMPADSPARQLPENAGFAGFR
                     FQESRFGHPGRRGETLDWRKNDWVAFLGASYFRAIGSDYQYGISARGLAVDPAVAGVA
                     EEFPDFTNFYIETPADGSNEVTVYALLEGPSVTGAFRFRMRRDRAVTMDIEKEIHLRR
                     DIQRLCIAPLTSMYWYSETAKPAAIDWRPEIHDSDGLAMWTGAGERIWRPLNNPPRTV
                     TSAFSDENVRGFGLMQRDRVTDHYLDGVAYERRPSLWVEPLGDWGKGTVQLIEIPTDD
                     EIHDNIVAAWVPAEPAKAGQSLSYRYRLYWQAEEPNPSPLARAVALRIGRGGEPGKPR
                     PEGRRKFVVEFLGEPLAKLPNGTLPEAVVTVSRGETSHVFTEAVPNDVPGHWRAQFDL
                     AAEGSEPVEIRLYLRLDGHALSETVLYQYHPF"
     gene            43121..43867
                     /locus_tag="VF226_11815"
     CDS             43121..43867
                     /locus_tag="VF226_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074720.1"
                     /translation="MAGVLIVTGGSRGIGAATAKLGAARGYRVAVNYNRNPASAEAVV
                     KEIEQSGGRAIAIQADVSREADVVRLFETVDRELGRVSALFNNAGVIHRMKPITDFAE
                     AELAEMWRVNITSQFLCAREAAKRMSTKLGGEGGAIVNMSSAAARLGGANGSLAYAAS
                     KGAIDTFTTGLALELAPQGIRVTGVRPGLIDTTIHNDMGDPDRLAKLRPTVPLGRTGR
                     VEEVAEAVLWLLSPQASYVTNTIIDIGGGR"
     gene            43986..46448
                     /locus_tag="VF226_11820"
     CDS             43986..46448
                     /locus_tag="VF226_11820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX7074721.1"
                     /translation="MIRTRTWVRRSLIGAGLVLAAAAGATAVVAIGTVPPLTGELAVR
                     GLSKPVSIVRDRYAVPHITGATLADVYRGIGFAHAQDRLWQMEVMRRAGQGRLSEIFG
                     ERTADADIFLRTLDLAGTAERSFARFPREAQELLKAYAEGVNAFIERPTGLLEQRLPP
                     EFLLLGYRPEPWRPADSLTIVKMMALHLSTNLGAEIQRLTLAAQGFSPAEIDDVMPLN
                     DGENPPPLPELATLYPLRSLEKTTPRSQTAALDVFLGAGASNNWVVAGSRTVTGKPLL
                     ANDPHLRLSAPSIWYLAHLALDRPGRERIDVVGASLPGTPVVVLGRSNTLAWGFTNTE
                     ADVQDLFIEKTNPDDPELYLTPDGWRRFDVDVMEIRVRGGETRLVERRRTRHGPVLPA
                     SFRNLGQILGPGHVAALQWTALSDDDTTILAGLLDENARTIFDYMERSRWYVVPMQNM
                     VVADTEGHIGMIVPGRLPMRHPDNKVAGRAPVPGWEAVYDWQGFVPFDELPRTVDPPE
                     GAIGTANARIADGSYPHLITYDWEADYRSRRVNELINGKTGHDLETMRAAQLDVFSPA
                     FAELAPLMIAAARPFVSSPNGQDILDRLSRWDAFMRADAVEPLLFMAWLRQSVRAIYA
                     DDFGAAFDQFYHPRAKAMIRLLRGEGSRDWCNSRSTKKTETCGEVLAAALKRAIEELE
                     AKFSSSRAGWSWGKAHVATGENQVFGQLPFVGRFFQIGLASPGGPYTLNRGVTEFGSD
                     APFANRHASTYRAIYDLSNLDGSLFMQPTGQSGNPFSRYYRTFEKSWAEGDYIQIPTD
                     PAAIERDAIGRWLLTPSADGAG"
     gene            complement(46642..47028)
                     /locus_tag="VF226_11825"
     CDS             complement(46642..47028)
                     /locus_tag="VF226_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074722.1"
                     /translation="MKAHIDSIFLIIAFLYFVAGMGLGIGMGIAEDFAYAHLHAHINL
                     VGFVAHALYGITHRLWPALRDSFLSTPQFLLAVCGAPVFLIGLPLAQFHGQPALAIIG
                     SLLLLASAILFLVMFATKLMRSTSPV"
     gene            47313..48248
                     /locus_tag="VF226_11830"
     CDS             47313..48248
                     /locus_tag="VF226_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074723.1"
                     /translation="MRKRFFWMLLAGTMLAAGGHAFAVATSGSESSGRTEAPASVRVD
                     QWPKSIDEFLAGNHLERSDVVLTRREWDPASYLIRWATKSPFSHSALIFTSPIHESGY
                     KSTFVIEATTDGVDLSNFRDYLEDKSAMVAIKRLRRDWFDADKQSRVRGLLLENIKAD
                     YNYWAIVRIVRDLWFGIQHTIEGQEQTVESFRDSDWKPPNSYICSGLVQVGFVEAISE
                     YVLRRQLPPWALNEVVFNPSAASRLPPPEAWKEAPPSLIDDVIPQFRVQLATELEAVT
                     PEDLARSEKLEWLYFIRDGKVHKVSSYDEVLELMK"
     gene            complement(48272..49837)
                     /locus_tag="VF226_11835"
     CDS             complement(48272..49837)
                     /locus_tag="VF226_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7074724.1"
                     /translation="MELSQNAIYVLLAGVALFLYAIAWWLTRRKGLLTRIIVRSALAL
                     MMLLPLAVGLQLGQMGSPDKSAAPEGRVEQEVGGADDQERPAAEWQTHGREFGTAPED
                     LARSRRIGSSKRSAAPKDGAPAARDPAGSPLSDDGERPFAESAPPAPTTDSGAPARRS
                     AEAPPAAGATPPPEESDWDVVPVFYGTDRAKLSGGKRVDYGAERGKRLQLGHALVTVP
                     KSHQVPEIERPWVYHIPFTKIVIWQEEEDPKKHFTIKEIRELGSLEFLELVRKRLAES
                     VAYKDHALVFVHGFNTSFEFAVFRTAQMAYDLKFDGAPFVYSWPSKGALGVQDYSYDR
                     ESAGAAEPYFREFLDLVVRETGAKSVSVIAHSMGNQLLLPVLRDMRRSAPEGVKISQV
                     ILAAPDVDRDSFEFLAREIQGISGGVTLFAAANDRALAASRQFWGGVPRAGDVPPEGP
                     IIVPGVDTIDVTAINTEMFSLNHSGYAEKTELLNDIRLLIQTGERPPERRIPILERVT
                     TSAGAFWRYPAMR"
     gene            50035..51033
                     /gene="meaB"
                     /locus_tag="VF226_11840"
     CDS             50035..51033
                     /gene="meaB"
                     /locus_tag="VF226_11840"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX7074725.1"
                     /translation="MKSSPKRTELSLSGLVDGIRRGDRAVLARAITLVESTRPDDQKL
                     AQDLLQELLPASGGAWRIGITGTPGVGKSTTIDQLGTNLIAAGHRVAVLAVDPTSRRS
                     GGSILGDKTRMARLAASPNAFIRPSPSSGTLGGVTRRTRETMLLVEAAGFDVVIVETV
                     GVGQSETAVADMVDFFLVLMLAGGGDELQGIKKGIIEIADMIVINKADGDNIERSKRT
                     AADYRAALNILTPRTATWKPPVLTASAVENRGLDTVWAKIAEHRRRMTDSGELQAKRQ
                     EQAVNWMRDMLQERLMEALKSRDHAAQRIAELEEEVRHGRMTAPRAVDEALRLLEK"
BASE COUNT         9271 a        16712 c        16188 g         8891 t
ORIGIN      
        1 taagtgggag ttccggccgg agcctgggac gcacgaggtt cgcgtcggcc tcatgggact
       61 tggcgtcatg ggagaggcga cggcgaaggc gctgaaggcg ctcgggttcc agctccgtgg
      121 ctggagccgc agcgagcgca acctcgaagg cgtggaatgc ttcgccgggc aggaaggcct
      181 cgatgcgttc ctgtccggca cggacattct cgtctgcacg ctgcccctga cgcccgacac
      241 gcggggcatc ctcaatcgcg gcctcttcag caagctgtct cagcacggac ggcatcagcg
      301 catgccgggg cccgtgctta tcaatgcggg acgcggcgcg ctgcagatcg aggccgatat
      361 tctcgcggcg ctcgatgcgg gcgagcttta tgccgcctcg ctcgacgtgt tcgagacgga
      421 acccttgccg gcatcgagcc cgctgtggtc gcatccgcgc gtcatcgtca ccccgcacaa
      481 cgctgccgaa agcacgcctg aggcgatctc ccactatatg ctacgccaga tcaggcgtca
      541 tcaggccggg gagccgctgg aaaacgtggt ggatcgggca cgcgggtact gacgccgacc
      601 cctcacatga cggatatcaa ccgttagaga actccggtgt gctagtcgcg gtcgagtcgc
      661 gctaggctca ggcctgcgaa ctccctccca aagcggcgat cccggcaatg gctcctgacg
      721 attccttccg caagcagctt cagcacgtca tcaccgcgct cgagacctgg gcgaaggaaa
      781 tggcgccctg ggcccagatc gagctcggcc ccatcggcaa tgcctggcgc atcagcgccg
      841 tgccgcatgc cgagacggcc tgccccttcg agatcgttct gcggacggac cgtcaattcg
      901 acatcaccgt cgacggtgag acctacgagg acctgccgct tgatgcgctg accgacattc
      961 cgcaaatcgt gcgcgccatc gccaacggcc gcattctcat ccgccgatgg caaagctccc
     1021 tcaccggcct ggtctacagc gtcgagacgg tcgtggatct gcctggcgcc gatgaagggc
     1081 ggttcacgcg ccgtaatcca gatgcgccgg acgtggatga tacggagctg gaggcagcga
     1141 tcgaaacgtt tgcgccatac cggcggtgat cgccctgatc tacttttcca gggcagacag
     1201 cgccggcagg cgtttgatgg cggagatctc cgttccggac ctcgcaatcc gttgcgcggc
     1261 ggtggccagc ggctcgaccg tgacggccat gtgatgggcg ttggcgtgca ccatcatgaa
     1321 ggcatcgcac atgatgccga cgtgacccgg ccagaagacg agatcgccgc gttcgaggcc
     1381 ttccaggccc ggtgtgatcg gcagtgcgac gccaagctgt gcctgctgca tgtcggtgtc
     1441 gcggggcgcc cggaggcctg ccgcctccat cgagacctgc accagccccg agcagtcgat
     1501 gccgacgcgc gtgcgaccgc cccaaagata cggcgttccg atcagccgtt cggcgatctc
     1561 gacgaagtca cgtgcgcagc ggctcttgtc tgacgcatgc cggtctatga cgaagccgcc
     1621 gccagcgagg cggtaaaacc ggtcgacatg ctcggccacg gcgagaggcg cattgatgct
     1681 gagatgcatg atcggcgcgg tcttgatgtc gggtgccgga taaaggaatg tgcccagcgc
     1741 ggagaccttg tgcgtcgccg gccgcacttc gggggtcagt gcgttggcag gcacgtaacc
     1801 cacatacccg tcccggtcga gttgcaccca agcccagccg ttggcctcgt cgtagacggt
     1861 aaccacttct ccgaaaagcg cctcggtatc gaggcccgcc gtcggaacgg gccggcgcct
     1921 tacggcaaca gcagcgcgga tcacctgctt cttgacaccg gcaacgtagc gcggggcctg
     1981 aaccttgccg cgcagcgact cggctgccag atcttcccgg taggcgttga gccgcggatc
     2041 gagccgcggc atctgtacgt ctggtctcac gacttctgcc ctgttccagg ctggtaggcg
     2101 cgccgcagca cctcgaacac ggcacgcgcc gtgtgtggct caccaccttt cggtccgagc
     2161 ggccgagccg acgggcgcca cccgtagatg tcgagatgcg cgaacgcttt ggtattgcgc
     2221 acgaagcgac gcaggaagag cgccgccgtc acggagccgg caaatgagcc ctcggaaaca
     2281 ttgttcatgt cggccacttc gctatccagg ttgcgctcgt agccattcca gaacggcatc
     2341 cgccacatcg ggtcgccaac ggagagcccc gctgaaacca actcggctgc gaatgcatcg
     2401 tcgtccgtat agagcggcgg cacatccgga ccgagggcca cgcgggccgc acccgtgagc
     2461 gtcgcgaatg agatgaggcg gtccggcccc tcctcgtcgg caagcgtcaa ggcatcggca
     2521 agaacgagcc gcccctcggc atccgtattg ccgatctcga ccgtcttgcc cgaacggctc
     2581 ttcagcacgt cgccagggcg gaacgcgttg ccgccgatgc tgttttccgc cgccgggatc
     2641 agcacgcgca ggcggacggg caatttcagc gccatgatca tgtgcgccag ggccagcgcg
     2701 gtcgccgcgc cgcccatgtc cttcttcatg aggagcatgc cgcttgcggg cttgacgtcg
     2761 agcccgcccg tatcgaagca gatccctttc ccgaccagcg tcaccctggg ggcctcggcc
     2821 gcaccccaga cgaggtcgat caggcgcggt gcccggacgc tggcccggcc gacggcatgg
     2881 atcatgggga atcctgccgc gaggtcatcg ccggtgacga cggtgagcga agcggcgtgc
     2941 cgctcagcaa gactgcgcgc cgccgcctcg atctcggcag gccccaggtc gctcgctggc
     3001 gtgttgatga ggtcgcgccc cagccaaacg ccctcgacga tgtgcttcac cgcagctttg
     3061 tcggcgccgg ccggcagaac cagcccgggt ggctcgtcgc ctccgttcga cttgtagcgg
     3121 cggaagcggt aggccccgag cccccaggcg actgcggcga cctccgctgc ttcgacctcg
     3181 tcggcaaagg cgtagccccc cggcggcagg ctcgacgcga gttgcccaac cagcaatgcc
     3241 gacgggccgc acggatcgcc gttctggcca ttacccgtgc caaggacgac gcccgcgatc
     3301 gcgccatcct ctccaggcag gaggacgtgc ttccgcgcgg cgcccttgaa gtccatgctc
     3361 cgcagccagg tccgctgcgc atcggtgagt ccgggcagaa cgtcgagctc ctgcccttcc
     3421 ccgagcagcc aaatgcgccg ggcctccttc gcctggtcga gaaacagtcc tgggggaatg
     3481 agattttctg cgagcatgtc ttttctggtg cctcggttgg atggcattca agccctatgc
     3541 ggtcccgagg gccagcgcca aacctgcgac atagcacatc agccgaatcg cccggcccgc
     3601 aacagggacg cgacagatcg cagtggattg ccaccgcgcc cgaccgacgc gccgctgtgg
     3661 cttcaagcgc ccaggcaaaa aagcgatcgc gctgcaacgg catttctgcc gccatgtttc
     3721 ccacaagaga ctatgatcgg ctccgtcctg ctggatcgag ctttggagcc caccaagatc
     3781 gagggaatac gatggctggc cgaaaccctt tcctgccgac actgagagcc accgcagtcg
     3841 cagtcctgct ggtgctcggg tcaacagcca atctgttcgc ggagggggcg ccggccaccc
     3901 agacgcggct cccgcaggac gtgataacgg agcacgtcgt cgagctgccg ggccgcaccc
     3961 tccggttcac ggcgacggcg ggctcgttcc cgctcaccaa ctccaagggc aaggtgctcg
     4021 ccgagatggg ctacatcgcc tatacgctcg acggcatgcc ggccgaccag cgccctgtca
     4081 cgtttgcgct gaacggcggc cccggatcgg cctcggcgtg gttgcagatc ggcgcgctcg
     4141 ggccctggcg catccgcatg gagcaggccg cagcctcccc ctcggcgcct gctccgctgc
     4201 tgcccaacgc ggagacgtgg ctcgatttca ccgacctcgt tttcatcgac ccgatcggca
     4261 cgggctacag ccacatcgtt gcgagcgaca atgccgacga tgggaaggac gagcagcgca
     4321 ggcgaacggg acgggccggc agcagggacg agggaggccc gagctacttc tggtcgatcg
     4381 aaggcgatgt ggaatccatc tcggaggtca ttcaaaaatg gatccagaag aacggtcgct
     4441 cggcctcgcc aaagctttat gtcggagaaa gctatggcgg tttccgcggc ccgaagatcg
     4501 cccgcgagct gcaaagtgag catgggatcg cgctgaacgc gctggtgctc gcttcgccgg
     4561 tgctggactt cgccggccgg cgcagcaccc atccgccgat ttcctacgtc tctctgctgc
     4621 cgtcgctcgc tgcggcagag atggagcgcc acggtgaggc gatcacgcgc gaggcgctga
     4681 gggatgtcga ggaatatgcc cggaccagct atcttgagga tctgatgcgc ggcccgcgcg
     4741 actcggctgc cctcgaccgc atcgtcgcga aggtctcgga aatcaccggc ctgccggagg
     4801 acgtggtgcg gcgccacggc ggacggatca gcgggcgcat ctatactgag cacgtcaacc
     4861 ggccggccgg gaagatcgcg agcctctacg acgcgagcat caccgggttc gatcctgatc
     4921 cgatgtcagg caggtcgcat tacagcgatc cgttcctgac ggcactgaag gcgccgatga
     4981 ccggcgcgat gctcgaactg taccaatcga agctcgacta tcgcacagac cgtccttacg
     5041 tgaagatcag cgggaaggtg aacaacagct ggatctgggg caattcacca accccacctg
     5101 aatcgatgag cgacctgcga gaggccctgg cgctcgacaa acggttccgg gtgctggtgg
     5161 tgcacggcta caccgatctc gtcacgccgt atttcgcgag cgagctgatc ctcgaccagc
     5221 ttccggcatt cggggacagg cagcgcgtca cgagcgtcgt ctatcccggc ggccacatgt
     5281 tctacagccg cgacgagtcc cgcaaagcct tccgcgagga tgcgttcaac cttgtcgagc
     5341 gcatcattgt cgagtcgaac gaggctgccc tgctctccgg gccgccccag acaacggggg
     5401 cagccgagcc tcaggaaccc gcggttccgg ccaatccagc cgagcctgct ttccctgagg
     5461 gggcggatga gccgccgacg gccccgaacg ccgaggcgcc gcctgaccaa tcgagcagcc
     5521 gcgatacgtc agacaggcgc gatccagacg tgattgtcgt gaaacgcggc cccgccgaag
     5581 ggggccggct gtatggcgct cgttaacgtg ttgatgcctt tctcgccttc gaactgagcg
     5641 ttgggagaga tgccttcgac gatgacgacc cggcgctgca cgccggcgaa ggcttccgcg
     5701 tggatcagga tctccccgcg ctcgttgccc atgcggatcc ggtcgccatt ggtaatccca
     5761 agctcggcca agtcatcggg gtggatcttc gcccgcggcc ggccttcgcg ggcacggctg
     5821 gagggcatct cgttgaatga cgagttcaga tagctgcggg ccggcgaggt cgcgagcttg
     5881 aaagggtgcc tctcgtcgat cttctcgttg acgtcccagt ggtcgggcag ctcggggggc
     5941 aaatgggcaa taccccagtc gtgcttgcgg ccgaccggaa cctcgggcca cttcggacga
     6001 aagcggaact tgccgtcggg atagccgaag ccgttgatgt agtgtgagct ctcgaaatcg
     6061 ggctggcaat cgatccactt cttctcttcg agctcctcga gcgtgcccca gccggatttt
     6121 ctcagcgtcc agtcgatgat ctcccgcgcc gtcatctcga agccgggatg ccttgcgccg
     6181 acgcgtttcg ccagctcgac gatcacgtcg tggttcgacc ggcactcgcc gggaggctcg
     6241 atgagcttcg gcccgagcac gatgctctgc tgcccgcccg cctgatagac gtcgtcgtgc
     6301 tcgagaaact gggtcgccgg cagtacgatg tcagccacct cggccgtggc ggtcatgaag
     6361 tgctcgtgca cgcacacgaa cagatcctcg cgtgcaaagc cctgtttcac cagccgctgc
     6421 tcgggcgcca cctgcaccgg attcatgttc tggatgaaga gcgccgtcac gggcgggccg
     6481 tcgccgaggt cgtagcgatc accggtcagc accgggccga tgcgggacat gtcgagcacg
     6541 cgcacgctgg ggtcggcgac gtccagcccc tcgatcagcg acttgtccca gtggaagatg
     6601 tcgccgttgt tgtggaacgc accgccgccc tcgtagagcc aggagccggt cacggaggcg
     6661 atgcaggagg ccgcgtgcat gttcaccgag ccgttgcgct ggcgggagaa tccgtagccg
     6721 agacgcaggt aggtgcgcgg cgtcgtgcca atcatgcgcg cgaaggtctc gatctcttcg
     6781 accgtcagcc cagtgatggc gctcgcccac tccggcgtgc gcgtccgcag gtgcgcttcg
     6841 agctcgtgcg ggcaatcggt atagcgctcc atatagggcc agttggcgta accatcccgg
     6901 aacagcacgt gcatcaccgc gcaggcgagc gcgccgtcag tgcccggacg gaggcagagc
     6961 gccagatccg cctgctccat cgttccgtta tgatagatgt cgatggcgac gatcttcgcg
     7021 cccctggtct tccgcgcccg gatcgcatgg gtcatcacgt tgacctgcgt gttgaccggg
     7081 ttggtgcccc agatgacgat cagatccgac ttcgccattt cccgcgggtc cgggccggcg
     7141 agcttgccgg tgccggcgac gtagcccgtc caggccaggg tcgtgcagat ggaagcgtac
     7201 atctccgagt agcgcttggc gtgccgcagc cggttgatgc cgtcgcgcat cacgaggccc
     7261 atcgtgccgg cgtagtagta cggccagacg gcctcgggtc cgtacttccg ctctgccgtg
     7321 agcatcgcct cggctgtgat gtcgagggca tcatcccagg agatgcgctc gaactcacgc
     7381 gagcccttgg gtcccttgcg gcgcaatgga tagagcagcc tgtccggatg gtgcacacgc
     7441 tcggcgtaac gggagacctt ggagcagatc acgcccgccg tgtaggcgtt atccttggac
     7501 ccgcgcacgc gtccgatccg gccgttgccg aggagctcga cctcgagcgc gcaggtggat
     7561 gggcagtcat gcgggcaggc cgaatagccg atgggaacct tggcgtccat gtttcgttcc
     7621 agtggaacaa ccgaagagac ttgaacgcta actgctttct tgaatggatg aaagcgcatc
     7681 gcgggcggga gcgcgcaagg gagcgtgaac acctgctgga gtgcgatgaa agccggcatc
     7741 accggacatc gcgaatggag accaaaatgc gcctgttcct tgcggccctg attgcgctcg
     7801 gcgggctagc ggcattgccc gatcacgccg atgcgcgcaa gaagccccgc gcgccttacg
     7861 cctatcgaga ctatggcccg ccgccggcct atcggaacta tcgacgggct cctccgccct
     7921 acggctatcg ctatcggcaa accgaggacc cgaattcttc ggtatgcatt gaagcggaag
     7981 ccctcgatcc cggcggcaac tacgcaggtt atccctgctg ggcacggcgg gctttcgctc
     8041 cgaagaaaga gttctgacgc cggaggccta ttcgggcatg aacggcaggt ccgtgggctg
     8101 cggcttggcc ccgagctggg tcagcaggca atgaacctgc ccgcgatggt gcgtctggtg
     8161 attgaacaga tgggcaacga gaacccactt gggctgcgtg aagttacggc ctgcagcgcc
     8221 tgaaaaccac gtcagatcgc ccgcgaagtc ttcaggctga agcgcatgcg cccaggcgac
     8281 gagcttttca tctgtcgcct cacgttccgc tttgagcacg ttccagtcct cgaagtagga
     8341 gaccgactcc gggatgcttt tcggcgccgg ggcgggccaa ccggccagcc ggtgcatcca
     8401 catccggtct ccccacagca gatggttcag cgttgcgtgg atcgagccga agaaagctcc
     8461 gcgcggctct cttcgctgcc tgtcctccag agtatccgca gccccataga tcgactggtt
     8521 ctgccagcga ttatagcggg ccataagggc gacgtgcgca ggactgatca tgcctcacgt
     8581 taccatgttt cgtcgcggct tgcggcctcg cgggccagga gtggcgacag aggtgggagc
     8641 gatcaggtcg accgctcgtc gaggcccgat cagccaacga tctcgttcag cccgaagttg
     8701 agcaaaatct cgcgctccgc ggaagcgggg ctatcggagc cgtgcaccga attctcgccc
     8761 accgagcggc cgaacatttt gcggatggtc ccctcgtcgg ccttggccgg gtcggtggcg
     8821 cccatgacct cgcgatacct agcgattgcg ttctcgcctt ccagaacctg gatcacgatt
     8881 ggccccgagg tcatgaaatc gcagagttcg ccataaaacg gtcgcgcttt gtgctcctcg
     8941 tagaacttct cggcctgcgc cctcgtccag cgcacgcgct tctgcgccac gatgcgcagt
     9001 ccggcctttt cgatgagcgc gttaatcgcg ccagtcaggt tacgctcggt cgcgtccggc
     9061 ttgatgatgg agaatgtacg ctcgatggcc atcgctgccc tctgtgtcca cttctgccgc
     9121 tcgttggcgc cgggttatag ctgccaccct gcaggccagc aagggaggcg tccgtgaaac
     9181 gttccgggga aaaggacgca gcgcaattgc cgcgtgccat ctttcgccac atcaacgtga
     9241 cgtttataag cggcctggcg accgatctcc gaagaactgt agctgggaag ccgagtatgg
     9301 cgcgcacggc agcgtcgcca tcaaaatgtt cagagtagtg attgccgatg caggccgaga
     9361 taccgacgat cccgattatt cggttcaggc ttcagaaacc gtcattttgc gtgtccgttc
     9421 gatgcaccgg aggtgccctt gctgccgggc ggacgcgcac cttatgctcg gcgtggacgc
     9481 attcgacaag gctgtgaatg agcgatggcg ggggcggctt ctcaaataga gtggtatatc
     9541 gcgcgagacg gacagcagta cggtcctctg tcggacctgg aaatgcgcaa gttcgtcgag
     9601 ctcggacacc tgcgcccatc ggatctcgtc tggcgttatg gttttccgga ctggcggccg
     9661 gcgcccgccg tgttcccgac cgctgcgccg ccgagaccgg agccgtcgcg cccgtcctac
     9721 cggccggagc cgcggcctca gcctcaacag ccgcggtacg agcccgcccg gccctcgtcg
     9781 cgcgaggcga atgagcatgg cgccgcgtcc gcccggacgg cggcggcgcc cgtggccgtc
     9841 tccaatccct acgaggacga tgacgacgat gacggcgacg atttcctccc ggagcagccc
     9901 cggaagggtt ggcggcgcgt tgcggttgcc gccattctag tagcgctgct cggtggcgct
     9961 atctggctgg ctgtcgatct ggtgcccagc cctgccaatc tcattgcgct gatcgaggcc
    10021 ccgaagacgc cctccggcgg accacctgat gaggccgcag gaacggaaag ctcgcccagt
    10081 cagacgctgc agtctgcgcg tgccgacgcc gcggtgccgg acggtctgtt cctgaacacc
    10141 ccattgtggc agctcatcaa acgcgagttt cccgagtggt acgagcagcg cgtgaaagag
    10201 gcagcgcagc tcgaggctga gggccgcgat cccgagttca tccagcgccg gctgacggag
    10261 aatgtcgtcg cgctgcggcg tcagcatgcc gaggaggcct tggcggcctc gccggggacg
    10321 ctccgaggca tcgcacagaa tttcctcgaa aacctgcagc ggctgtcggc ggaaagtgtg
    10381 gaagcctgct acggcttcat cagcaagggc gaggcgagcc cccaggtgtt ggcgctgatg
    10441 aactcgccgg agcaccgtga acggctgcag cgccaggtga cagccgtgtt cgaggccgtc
    10501 gccgagggcc ggcgttcgcc gcagacccat ctgccgccgc gtcaggcgga ctatcaggca
    10561 ctgacacagg cgcttgccgg ccgcggctgg acgaacaacg acatccagct tttctccaac
    10621 ccgcgcgcgc tcgcccaagc gccgccgcag gaggtctgtc ggctcgtcca ggattggttt
    10681 gccgctcagc tctcgatccc cgacgaggac gtgcaactcc ggttgctcgt cgagtcgctg
    10741 aagccggtgg tggctggatg actgagcacc ccgctgcggt ttgacggcgt gatccccgcg
    10801 acggcagatc tggattcgag gcagaagccg cagccgggcc cgttgctatt tggcggcagt
    10861 gacatccgcc gcggcacccg ctatctcccg ccggatgctg cacatcaacg acctcacctt
    10921 ccgcattgag gggaagatca tcttcgaagg tgccacggca ggtatcccgg ccgggcacaa
    10981 ggtggggctt gtcggacgca acggcgccgg caagacgacc ctcttgcgcc tgatcacgga
    11041 ggagctggcg cccgacggcg gctcgatcag ctacccgaaa cacacccgga tcgggtacgt
    11101 ggcgcaggag gcgccgggcg gcgacgagac gttgatcgac tgggtgctat ccgccgatcg
    11161 tgagcgcacc gagcttctcg ccgaagccga aacggcgacc gatccccacc gcatcgcgga
    11221 tattcaccag cgtctcgccg acatcgatgc ccatgccgcg ccggcacgcg ccgcccagat
    11281 ccttgccggt ctcggtttcg acgaggcagc ccagcggcgc ccctgccggg agtactcggg
    11341 aggctggcgc atgcgcgtgg cgctcgccgc ggtgctgttc cttgagccgg agctactgct
    11401 gctcgacgaa ccgaccaact atctcgacct cgagggtacg atgtggctcg agagctacct
    11461 cgccgcttac cctcacaccg tcctgatcgt cagccacgac cgtgatctgc tgaatcgcgt
    11521 ggtcggctcc atcctgcatc tcgaccgcgg caagctcact ctctacacgg gcaactacga
    11581 cgaattcgag gatctgcgcc gcgagcggct aagccagcaa ctggcgctca agaagcggca
    11641 ggacgaggag cggcggcgga tcgagacctt catcgcccgc ttcaaggcga aggcaagcaa
    11701 ggcggctcag gctcagagcc gggtgaaagc cttggcccgc atgaagccga tcgtggctca
    11761 ggtcgaggag cgggtcgtgc cgttcaactt tcccaaggcc gaaaagccgc tggcgagccc
    11821 gcttctgcgc ctggaggacg tcgcggtcgg ctacgagccg gacaagccgg tgctcagccg
    11881 cctcaacctg cgcatcgaca acgacgaccg catcgctttg ctgggccaga acggaaacgg
    11941 caagagcacg ttcgcgaagc tgctcgtggg caagctcgac ccgatggcgg gccgggtgat
    12001 caaggcccat cggcttgagg tcggctactt cgcccagcac cagctcgacg agctggtccc
    12061 aagcaaaacg ccctacgatt acatcaccga gctcatgccg gatgccacag aggcgcagcg
    12121 gcggacacgg ctcggcggct acggcttcgg catcgagaag gccgatacgc gctgctcgaa
    12181 cctgtcagga ggcgagaagg cgcgtctgct gttcatgctc accgctttcc acgggccgca
    12241 ccttctcatc cttgacgagc cgacgaacca tctcgacgtc gacagccgcg aggcgctgat
    12301 gcaggcgctc aacgaattcg aaggcgctgt catcctgatc agtcacgacc gccatctggt
    12361 cgaagccagc gccgaccgtc tgtggatcgt gcgcgacggc acggtgacgc cgttcgacgg
    12421 cgatatggac gactacagac agctcctcct tgccgaacgg agcgccagga accgggaacg
    12481 tgcgaaggac ggagccggat cggagccgcg cgtcggccgc gccgagcaga ggcgggctgc
    12541 ggcggaacgg cgcgccgagc ttgcgccgct caaaaaagca atgcagcagg ccgaaaagga
    12601 ggtcgagaag atcacggccg agattgcgag gatcgacgcc gctctcgcag ataccgacct
    12661 atacgcccgc gacccggaca aggcacagcg cctgtcgatc gatcgtggtc tggccgcaaa
    12721 gcggctcgcc gatgcggaag aagcctggct ggccgcgatt gaggcctatg aggccgcggc
    12781 ctcggaggcg gatgcggtgg cttgatccaa caacgccgca cctatccggt cgtcatcccg
    12841 ggccttgggc ccaaaatcca gccaaccgcg agcgcagaag caagcttaag ggggatcccg
    12901 gtaaggagca ccgggatcgc gtctggggct gaagcaacgc tgtcaaagca aaagatgcga
    12961 cccgtcgcag aagggctgat cgtcggtcgc cttgcagccg caaaggttca cgatccctga
    13021 ggtttcggcg acgaaccgga cgggctcgat gccggtgtcc ttgtgcgagc catcgcagaa
    13081 cggctgcttc tgagagcggc cgcaagcgca ccagagataa acccgcccct cctggagctc
    13141 cacctgatag ggccccttct gagccacaat cggttcctca gccatcccct tatgatctcc
    13201 ctggccaatg ctcgctggca ttgtgcagca tcgtcgggcg ccgcgtaacg gggtcgcgac
    13261 gggctgccgc gctctttttt tactaccggt tcccgcgccg aattgcctag tagtagcggc
    13321 gagcagaaaa ataccaccac accggggact catcatgcgg catggcggca agatcctgat
    13381 cgatcagctc gaggcgcaag gcgtgacgac ggcattcacc gttccgggcg aaagcttcct
    13441 cgccgcgctc gacggactgc acgattcaaa tcagatccgc acgatcatct gccgtcagga
    13501 aggcggcgcg gcgatgatgg ccgaggcctg ggggaaggcg accggcaagc ccggcatctg
    13561 cttcgtcacg cgcggacccg gcgccgcaaa cgcgatgagc gggcttcacg tggcccagca
    13621 ggactcgacg cccatgatcc tgttcgtcgg cctgcccggc gagcgccatg aggatcgcga
    13681 ggccttccag gagatcgaga cgaagcagct gttctcctcg ttcgtgaagt gggccgccgt
    13741 cattcgcgcc accgagcgca tcccggaata cgtgagccgc gccttccacg tggcatcgtc
    13801 gggccggcca ggccccgtcg tgctcggatt gccggaggac atgctgtcgg cgaaggcgga
    13861 cgcgaaggac gcccgcgccg cgcgtatcac gcaacccgca ccaagcgcca cgacgatggc
    13921 cgaattcgcg gatctcctcg cgaaggccga gcggccgctg atgatcgtcg gcgggcccgg
    13981 ctggagcacg gatgtgcagc agcggatcga gcggttcgtg gagcgcttcg atctgcccgt
    14041 cgcggcagcg ttccggttcc aggactacat cgacaaccgg cacccctgct acgtcggcca
    14101 cgtcggaatc ggcatcgatg ccaagctcgc ggcggccgtg cgcgatgccg acctgctgat
    14161 tgcactcggc gcgcggctgg gcgagatgac cacgagcgga tacacgctgc tagacattcc
    14221 gaacccttcg cagcggctcg tccatgtcca cccgagtccc gacgagttgg gcacggtcta
    14281 cgcgcccgat ctgccgatca caacgaccgc ggagcagttc gccgccgcac tcggtggctt
    14341 gacgccgccg gccaacatcg cctggaagca gcggcgcgca gatctgaggg ccgcatgcga
    14401 ggcggcctgg acgcccatcg ccacgcccgg cgcggtccag ctcgagcagg tcatccgcac
    14461 cgtctcggac atgcttccgg aggacggaat tgtgaccaat ggcgccggca actacgcggc
    14521 gttcgtccac cgctacttcc agtacaaggg ctaccgcacg cagctctccc cgacgtcggg
    14581 ctccatgggc tacggattgc cgtcggccat cgccgcgaag ctcgccaatc ctacgcgtcc
    14641 ggtggtttcg ttcgccggtg acggatgctt catgatgacg agccaggagc tcgcaaccgc
    14701 agtccaatat gggacaaacg tcgtcaccat cgtgtgcaat aacggcatgt acggcacgat
    14761 ccggatgcac caggagagaa agtacccgaa ccgcgtcgtc ggcacgacgc tcgtcaaccc
    14821 ggatttcgcc gccttcgccc gggcctttgg tgcacatgga gagaccgtgc gccacacaga
    14881 agagttccgc ccggctttcg agcgggccct ggccgcgaac aaaccggccg tcatcgagct
    14941 gaaaatcgat gccgaagcgc tgaccccgcg gcagtctctc agccagatcc gcgcatcggg
    15001 tgcgaaatct taagagagtc gaaacacgtc actgagttcg cgttaaacgt gtttccagga
    15061 cacattcgac agatatcgcg ctatcggtta caagttcagt aacgatctcg acgcgctgaa
    15121 atggctccaa cggagattcg gcaccccaat tgcaagtagc agcacgatcc gctgctacgg
    15181 ggtgccggtg agccagccgg tctgcgggtc tatgagcgcg acccgatcgt ggttacgcct
    15241 gatgaactcc tcacgcggta ttcttgacga ctctccccct ttgccttccc ctcctccggc
    15301 cggaggaggg ggtttctttt tgtattgccg taagttccgc acctcacctc atatctcttc
    15361 cgaactcgtg gtaaggcaac agcggcagcc cctccacctc tcccacgggg agaggtcggt
    15421 cggagagccg ggtgaggaat tgcaacagtg gttagcggtg gagccccacc accccgcccc
    15481 ctccccaccg gggagaggga gatgaggcac gagcttagct tggcttgttt cgatctacat
    15541 cggaaatgat ctaggcggcc tcggcgaagt cgagccggaa attgtgctga tcggcgagga
    15601 tcttgatggc ccgctgagct tcttccgccg gcagcggagt gaacggcggg agcacctcgg
    15661 cccagccctc gtcctgccga taatgtgcga tgagcgcctt cagcgtcgga atgaccggga
    15721 aggactgaac ggtcttccgc aaagcagaga ttttctcctg catcgcatcg gcattgggcc
    15781 cacgccagtt gtcgtagagc tggcgcatgg cacgggcgct gatgttcgcc gtcgcagaga
    15841 tcacaccggc cgccccattc cgcagtccgt cgagaagaaa gagctccgag cccgggaaca
    15901 gctcgaaatc cgggaaggcg tcgaggatcg ccttcatgtt cgaccagtcg cccgaggagt
    15961 ccttcaggcc gacgacgacc ccaggaaact cgttcttgag ccgctcgatg agcgataggg
    16021 agaagcccac ctgggcaacg ggggggatgt ggtagaggta cacgcgcagc ctgtcgtcgc
    16081 cgacctcgtc gatgacatcc gcgaagaacc ggaacagccc ctcctcgctc gggttcttgt
    16141 agtagaaggg cggcagcatc aggaccccgc cgcagccgag gtcgacggcg tgctgggtca
    16201 gcatcgcggc atccgcaacc gagcaggcgc cggtgcccgg catcagcttc gaaggatcca
    16261 tgccgctgtc gaccagctcc tccaaaagct ccatgcgctc atcaaggcca atggaattgg
    16321 cctcactggt ggtgccgaac ggcgccagcg ccgtgcagcc atcctccatc agccactctg
    16381 cgtgctcgat gaagcgatcc ggatccggag cgccatcgtc gccgaaaggc gtcaacaccg
    16441 gcgcgatgac gccattgaaa ttgcgcggga cgttcatggt gtctattcct cgtagtcctg
    16501 aaacttaaaa ccttctcgat gcccatatcc gtcgcgatca aggcatcgga atcgagggct
    16561 gcggcaccgg aacatggtag cccttctgga ccgcgggccg ttccgctatc gcacgatacc
    16621 agcgcagcac attgggaaac ttgtcgaggg cgatctcctg ccattcgaac cgggatatcc
    16681 atggccaggt ggcaatgtca gcgatggagt aatcacccgc caaaaactcc acctccccaa
    16741 gccgccgatc gagcacacca tagaggcgca gggcctcctt ggtatagcgc tcctcggcgt
    16801 acggtgcctt gcccctgttg aatttcacga aatggtggac ctgtcccaac atcggcccga
    16861 ccccgcccat ctgccacatc agccactcga cgacgcgcca gtgggcctcg ccttgcttgg
    16921 gccaaagctt acccgttttc tcggcgagat acatgaggat cgcaccggat tcgaacagcg
    16981 acatgccgtt gtcgcgatcg acgatggcag ggatgcggtt gttggggctg atcttcaaga
    17041 aggcaggctc gaattgctgg ttcttgccga tgtcgaccgg atgcaccgtg tagggcaatc
    17101 ctatctcctc cagcatgatc gagactttcc gcccattggg cgttgaccac gtatagagat
    17161 cgatcatcgc gagcacgtcc ttcccgtcag gtgatgcgag cggcggagcg tgatgatcga
    17221 gaagttcgcc tgttctctcg gcgcccgcct agggccagct tcacgatccc gatcacgctg
    17281 gaaacttgtg tgattttgat gtcccatccg atcccgaagt tccctccgac cccgccgcaa
    17341 gctccggcga gctgcggggc cgcgacacta gacgatacca ccctgccacc gcaatgctac
    17401 cggtggcaca tcacgtgagc cgtgtcgcca gaatccacgc cgttacgaac agcggtgaaa
    17461 gtgccgtgga aagcaccacc gctgtcgaaa tgaactccga ctcggtcttg tactcggccg
    17521 caaagatggt gatcagcacg ccgactggcg tcgctgccgc gacgaccatc accgccgtga
    17581 tgtccggcgg caaacccatg gcatggcaaa tccaccagct cagcgcaggc gccgcgacga
    17641 atttcagcag cagcatcagg gtctgtggga gagcttcgaa gttcaccgcg cgtgcggcgg
    17701 cgacctgcgc acccagagta taaagcgcca gcggcgtgaa gattgaaccg aggaactgca
    17761 acggctggcc gacaagggcc ggcagctcga cctccagccc gcgcagcgcg atgccgagga
    17821 tgaccgacgg aatgagcggc gtctcgaatg cgttgcgcaa tttgacgagc cggctgccgc
    17881 cggccggcgc cagcagccat acgcccaccg tgcagacgag gatggccatc agagccgtca
    17941 gcactgactg gtagatgagg taatccgcgc cgaacgcgag ctgtgtcacg ggaatgccga
    18001 agaagccgac gttcgcgtag gccgtgccga gccccatcat cggccccagc gagggtcgca
    18061 tgcgcagcaa atgcacgagc agccatccaa gcggaatgag gatgatgaac tgcacgacgc
    18121 tgaagtaaac gaccggccag atcaccgaca gcggctgatt tccggtcgac aggaaatgca
    18181 cgagaaaggc gggcatcacg acgtagacct gcagcctgtt gaggctgccg acgtcgagct
    18241 tgaggcgcgg ctgcaggagc cagcccagcg cgacgagcgt gatgatcggc agagtgacct
    18301 gcgtgaggat ctcgatgaag agcgacatcc cctccccgcc gctgtcgctt gaactgctat
    18361 ctgctgagcg gctgccgtcc gccgcccaag aggtccgacg ctgcttgcag aaatcgcggc
    18421 cgaatgaaac ggctatggtg ccgcaggcgc cgcgtgcgga gacttcgagc gggcggggga
    18481 gccggcgcgg ctccgccgag acgtgctgct gccggcagtg ctaggagcga tcagcccggc
    18541 ctcctgctct atccttgcca gcgcctcgct cagtgcgcgc accgcggacg gcatgtcccc
    18601 tccgtgtggc acagcaatgg ttctcgcagc gcctcgtgat tcaaggcgct ctgcgaggcg
    18661 cggccccacg atgcggcgca gacaggcttt cggcgtcaat gtcgcgcgta ctgcatcgag
    18721 ctggccggtg agccgcagcg cgcgtacatc agggcagacg tcatagagct cgccgacgag
    18781 gttgagcggc gcggcgatca ccgctgaacg gcctgagatg agcatcctct gaatgctgtc
    18841 cagcaggccg aacctgtgtc cctctgagcc ccatgtggcc atgaaaccgc cgtcctgctc
    18901 gatctggcgg aacaggctcc gggtcacggc aatctctgcg tccggaggtc cgtcgcgccg
    18961 ggtcgtgatg cgcttcggaa actccaaggt gctgtggggt atgcgccggc gcgcggcggt
    19021 cagcaacaga tcgagctgag cctgctcggc ggctgtgacc acgaccaggc agccggagtc
    19081 gccggccatc ctgtcgcgag tgaaatgctg cttcggttgc gccctgcgcg ccatggagcg
    19141 gtcctgactg ccatcgaggc gagagtacgc cggcaccagc ggcgcccccc aaagccccct
    19201 gcatctgaac cgactcggcg tggcttgtcg atagatggga ggtatgcgct cgcagaggaa
    19261 ctcgtggacg ggtccggaag cgacacagtt tcgccgcaat ccagccattg atcggtttgg
    19321 ttcgcgttaa gcgtgtatca gtggcgtacg gactaccttg ggtgaacagt cgggacctct
    19381 cgcaacatca acggggtaag cggtatgacc atcgcttcag gcgccatcat ccattcgttg
    19441 ctcgatagct tcgccgccgc ggaggtgcgc gactttccct acaggcattg gctgctgacg
    19501 cgctgctttc cggaagaggc ggtcgacgag gtccttgcgc tccccttccc ggcacccgcg
    19561 ctcggcggca tctccggcaa acgagagatc cataatgcca cgcggaaata cttcgacgtc
    19621 gagaaccgcg ggcgattccc catcgtggct tccgtagcgg aggccctgca ggacaggcgt
    19681 gtgaccagcg cggtggagcg cgtcttcggc gtcaggttgg gcggctctta tctgcggatc
    19741 gagtatgctc aggataccga cggcttctgg ctcgagccac acaccgacct cggcgtcaag
    19801 ctgttcacgt tcctgatcta cctgtcgaag gatccgagcc acgccgatct cggcaccgac
    19861 atctatgatc tggaaaaacg gcacgtcggc cgctcgcctt tcgtttcgaa cggtgcgatg
    19921 gtgttcgtgc cgtcggacat cacctatcac ggcttcgagc cgcgcaagat caacggcgtg
    19981 cgcaaatcga tcatcgtcaa ctacgtgacg ggcgcctggc gcgcccgcga gcagctcgcc
    20041 ttccccgaca atcccatcgc cagcatggcc gcggcagcct gatcggggcc tctacgctgt
    20101 cgccgcctgc cggctcccgc gccagtgggc ggcgatggcg ccagccagca tcaatgctgc
    20161 gagcaccagc agccccgccg agatgggtcg cgtgacgaat acggacaagt cgccctggct
    20221 gatctgcacg gcccggcgga actgaatttc ggacagtggc cccaggatca ggccgacgat
    20281 gaccggcgcg atcgggtaat cgagccgccg catcaggcag cccagcaggc cgatggcgat
    20341 caaggccatg acgtcgacca gcgagcgatt gatcccgtag acgcccagca tcgcgaagac
    20401 ggagatgccg ccgtagagcc agggctgggg aatatagagc agccgcaccc acagcccgac
    20461 cagcggcagg ttgagcacca gcagcatcgc gttgccgatc agcaggctgg cgatcaggcc
    20521 ccatacgagg gcgctgttcg acgagaacag cagcgggccg ggctgcaagc catactgctg
    20581 gaaggccgcc agcatgatcg cagccgtcga ggatgtcggc aggccgaagg tgagcagcgg
    20641 caccaaaacg ccggcaaccg cggcgttgtt ggcggcttcg ggacctgcga cgccttcgat
    20701 tgcaccctgg ccgaactcct ccggccgctt cgccagtgat ttctcgacct tgtaggagat
    20761 gaatgtcggc agctcggtgc cgccggccgg cagggcgccg aacgggaagc cgatcagcgt
    20821 gcccctgagc cacggcttcc aagaccgcgc ccagtcctcg cgcgtcatga aagcccgccc
    20881 cttgaccggc acgatctcct cgcggcgcag gtcggcccgc gaggcgagga agagcgcttc
    20941 gccgacggcg aacagggcga cggctacgag cacgacaccc aacccatcct gcaactcgag
    21001 tacgccgaat gtgaagcggg actggcccgt ctgctggtcg atgccgacga gcccgatagt
    21061 aatgccgatg aacagcgaaa cgagcccccg caacaccgag ctgccaagca gcgacgagac
    21121 ggcagcgaag gcaaccacca tcagcgcgaa ataatcggcc gggccgaaca gcagcgcgag
    21181 cttcaccacc tcgggtgcga gcagcgccag aaggagcgtg ccgatgacgc cagccacgaa
    21241 cgagccgatg gcggatgtcg ccagcgccgc ggctccgcgt ccggcgcggg ccatcttgtt
    21301 gccgtcgatg gtggtgacca gcgagcccgt ctcccccggc gtgttgatca gaatggctgt
    21361 ggtcgagctg ccgtacatcg ccccgtagta gaggccgccg aacatgatga acgccgccgt
    21421 cggatcgaga tggtaggtga ccgggagcag cagcgcgatg gtcagcgccg gacctattcc
    21481 cggaaggatg ccgatcgcgg tgcccagcgt tacgccgacc aatgaccaca tcagattgag
    21541 cggggtcagg gcgaccgcga acccgtgggc aaggttgccg aaaacgtcca tccctcaccc
    21601 accgagcagg ctgtcgaata cggacccgac gggcaacaag agcccgagcc cgtaggagaa
    21661 gacgagaagg acaacgagac ccatcaggag accgacgccg aggtcggcga tcagccgccg
    21721 cgatccgaag gcgcgggcga ccgctgcgaa gaggagcgcg gtggccaccg cgaagccaag
    21781 agcttcgacc gtgagcagga agagcaccgg gccggcgagc gccgtcagaa aggctggcca
    21841 gtcgttttcg ggcgtgtcat tctcgcttcc tgcctcgggg ccgtcgtgcg agccgaataa
    21901 tgccgaggca atggtcagga caccaagcac caccagcgcg accgcgacga tcgacggaaa
    21961 gagccgcgga ccgagaagca ccacggcggg ggaaacgtgg atctcctgcg cgctgatgaa
    22021 aagtccggcg ccgatgagta cgagtgccag cccgaccagc gcgtgcgcgt tgaagcgacc
    22081 tgtcacgagc gctcctcaaa gaggtaggct gccgccactc aggcggcagc gccacgaagg
    22141 accgtggcta gttcaccagc ccgaccgact tcaagatctc cgtcacccgc tggttctcct
    22201 cggccaagta tcgctcgaac tcagggcccg agagatagtt gtcgagccag ttccgttcgg
    22261 tgagcttctg cttccactgg tccgacttca ccatggcatc gatgacggcg gtcagcttct
    22321 ctcgatcggc ctgcgagatg ccgggcggtg ccatgacggc gcgccagttg ccgatcacga
    22381 gatcgacgcc ctgttccttg aaggtcggcg cgtcgatgcc cgcgatgcgc tgatcgctgc
    22441 tcacccccag ccagcgcagc ttgccggctt ggacctgcgg ctcgagctcc gaagcgctgt
    22501 tgatgccaag cgtgacgtgt cccccgagga tcgaggccat ggcctcgccg ccaccggaat
    22561 gcgcgatgta gttgaccttg gcaggatcga cgccgacggc cttcgcgaaa agtgcggcgg
    22621 tgatgtgatc cgtgccgcca gcagaaccac cgccccacga gacggagccg ggatcggcct
    22681 tgagcttggc gataaggtcg ccgagcgtct tgagctcgga atccgccgga accgcgatgg
    22741 cctcccactc ggaggtcagc cgcgcgatgg gtgtgacgtc cgtgagcgtg accggcgact
    22801 tgttcgacag aatggccccg accatcacga gcccggacac catgagggat ttgccgtcgc
    22861 ccttcttctt ggcgaactgc gcaatgccga ccgtgccgcc tgcgcccgcg acattctcga
    22921 cggtcacgga cttcacgatg cctgccgact gcaggacatc ctgcatgacg cgggcagtgc
    22981 cgtcccagcc accgcctggc gcggcgggcg ccatgatcgt gagctgatcc atctgctgtg
    23041 ctgacgccgg cagcgccatg gcagcggcca gcgcaatggc gaagaacgtc cgccgagtgg
    23101 tccctttgcc cattcgattc ctcccagtca cgtaaagggc tggatgatcc ctcccgtcat
    23161 gactggtgta gtctctggcc aagcgggcag gcaaccccga cctgagtcaa aacgccggcg
    23221 caaatcgccg atgcgcaaca ggcaagcacc gcgagcctgt gacgactcgc ggtgcctcgc
    23281 agttcgagaa atgctggttt gggaagctag cttcagcgac cctgcggtac gctcggtcca
    23341 gccggcggtt gaaggcgatc gagaatgccg ccgcgcggcg tggtgtcctg ctgattttcg
    23401 cctgccggtg cctcggttcc ctgctgctgg gccgcaggct gatcgaagat cgacggcggc
    23461 gccgaagcgc gctgagccat cagcgacaaa atggtgctcg tcaggaaaaa ggctgccgcc
    23521 agcccggccg tgatgcgcgt cagaaggttg gccgtgccgc ggcccgtgag aaacccgccg
    23581 ccgcctccgc cgccgatgcc aagcgcacca ccctccgagc gctgcagcag gacgacgccc
    23641 acgagcgcag cggcgatcat gatatgaata aggaggatga cggtgaccat atcggttcgc
    23701 ttccggtaat cgggctgttc ggagattgcg gccttctaca cgaatgcggt accggccgcc
    23761 acccctcctg tcgccgcagc cgtgatgaga ttatcggcct gcacggggct tccgcttggg
    23821 cgcaggtgct gcggcatgcg ttttcggctt gcgcttgggc agcggcacga cgttggaggg
    23881 ctccgatgct gtggccacca cgtccgggca gcgaaacccg atctgctcgt cgagctcgat
    23941 cagccgctgg acggccttca gctgagccgg cgtcagattg gcacgccccc actccggtcc
    24001 cctcgccatg ttgtccctgg ccccgtccat gacgaggttg agccgctctt cccggagctg
    24061 aatgcaggtc tgagggtcga gcgggaccgc gctcgcagga tcagccggca gcaacgccgc
    24121 tgcagcagcg agcggcagga ggctgtaaag agcgcatctc aaatgcattg acccactcct
    24181 tcggtgccgg acaggcatcc cgcattacta cccgaccttg caggccgcaa cgatcccgag
    24241 cagatccttc gccttgaggc tcgcaccgcc gacgagcgcc ccgttgacgt gcggcactgc
    24301 cagcagctcc gcggcgttcg acggcttcac cgagcctccg taaagaacgc gcatctcccg
    24361 cccctgttgc ccgagcaaat cggacaggcg ggtccggatg aaggcatgga cctcggcgac
    24421 ctcgtcgacc gtcggcgtca ggccggtgcc aatggcccag acgggctcat aggcgatgat
    24481 cgtgtcggct gccgtggagg cctcgggaac cgagcctgag agctgccgcg cgaccacgtc
    24541 gagcgtcagg cccgcccgcc gttgccctgc agtctcgccg atgcagatga tcgccgagat
    24601 accggcccgc agcgccgctt ccgccttggc cttgacgatg cggtcgagct ccccgtggtc
    24661 agcccgtcgc tcggaatggc cgacgatgac ggccgttgca ccggcatcat gcagcatctc
    24721 cgccgagatg tcgccggtat gagcgccgga ggggagccag tgacaatcct gcgcgccgac
    24781 ggcgagtcgg ctgccatccg ccagttcggc cagtgcagaa atcaatgtgg ccggcgggca
    24841 gatcatcacg tcgatgccgt gtgagacctg tcctgaaacg atcgcatcgc gcacggctgc
    24901 cgcctcggcg aggtctgcct tcaggccatt catcttccag ttgcccgcga tgagcggcct
    24961 gatcggggct tgataggcgg tgctggctgt catgtcggca tccgtggagt ctctttgacg
    25021 cgtacgcaac gcggcagcgg gctgatatga tcgagcgatc ctgccttgca ttcaaggacg
    25081 ggctgggctt cttatcacat ccaagccgat tggggcgtag cctggtgggc acgccgttac
    25141 tcccttgaat cgctgaccgg caggcataac agaacgcaag gataagattt aatcgatgct
    25201 agacgccctg cgccgcggcg ccaccagctg ggtggccaag attttcctgg gcctgctggt
    25261 cctcagcttc gccgtgtggg gcgtggccga cgtgttccgc ggctataacg aaggcgcact
    25321 ggccgaggtc ggcgaccggc aaatcacgtc cgacgagttc cagcgcgcgc tgcagcttga
    25381 gctggcgatg ttgggccgcc agatcgggcg gcggccgacg atggagcagg cgcgcgcgtt
    25441 cggcatcgac acgcgtgtgc tgtcgcggct catcggggca gccgcgctgg attcccacgc
    25501 cgaggagctg cggctgtcca tcaccgacga agccgtcgcc gaggaaatcc ggaacgaccc
    25561 ctcgttcaaa aatccggacg gcagcttcaa tcgcatggcg ctggagagcg tagcgcgcga
    25621 gctgggcctc agcgagtacg gattcctcgc cctccggaag aaggaggaga tccgccagca
    25681 gctgacggat gcgatcaccg cgggtgccta tgtccccgat acgctcgtga aggcgcttta
    25741 cgactatcgg aacgaagcgc gcaacgtcga gcactttacg atcgatccca atgttgccat
    25801 caagctgccg gagcccgatc cggcgaagct caaggagctc tatgagtccg aaaagcagcg
    25861 cttcatgacg ccggagtacc gcaagctggc ggtcatcggc ctcaccatgg aggaaatgaa
    25921 gaagcgcgtg ccaatcaccg atgccgagct ctctgccgcc tacgagcagg acaaggcgcg
    25981 ctacgaggtg ccggaacggc gccgtgtctt gcagatcgcg ttcaagaccc cagaggaggc
    26041 agagcaagcc gctgcagcga tcaggggtgg aaagagtttc gaggacgtcg ccaaggaagc
    26101 agggatagtc gagtcggact acacgctcgg gctcctgtca cgcgacgagc tgatcgatcc
    26161 ggctgtggcc gaagccgcct tctctctcga aaatggcgcc gtttcggacg ttgtccgcgg
    26221 tcgtttcgcc ccggtgctgg tcaaggtcac cgagatccag cccgggaaac agaggacgct
    26281 cgacgaggtc aaggaccagg ttcgcgacac gctcgctgcg gacaaggccg gaccggagct
    26341 gaacaggttg catgacgaaa tcgatgaccg gcggttcgcg ggcaagccac tgaaggagat
    26401 cgcccaggag ctcgacctcc ccttcctcga aatcgaggcc actgacaaga cgggcaaggc
    26461 gccggacggt tcggtggttg tgaagggtcc ggatgccgac cggatcctga aggccgcgtt
    26521 cgagggtcag gtcggtttcg atggcgactc gatcgagctc gccgacggcg gctacggctg
    26581 ggtgaacgtg atttccgtga cggaagccaa gcaaaggaca ttcgaagagg ccgaggccga
    26641 cgtcgccgcg ctctggcgcg aacgggagac gcggcggatg ctctcggagc ttggcagcca
    26701 gctggccgag cgcgcgggca agggcgagag catggaggcc ctggcgcagg aggtcggcgg
    26761 caaggtcgag accgcgcgga acgtcaagcg gatcggcggt gcaccagggc tgcccgacgg
    26821 tgccgtgggt caggccttca tcacggcccg agacgccgcc ggctcggccg agacgcgcga
    26881 cgggaaatcc cgcgtggtct tcaaggtcac cgaggtgatc ccgccgccgg cggcgaccaa
    26941 ggaggaactc gatcgcttgc gctcagagct cgagcggcag gtcgaaggcg acctcctctc
    27001 cggctacctg gggggcctgc aggaccgcct cggcgtgagc atcaaccagg ccgcctatct
    27061 gcgcgccgta ggcgccagcc cagagcagca atagagggat gcatcggtga gcaagcccgc
    27121 gatccccggc ctcgaggtct cgccgggtta tacctctttt gctcagaagt acgagaaggg
    27181 tgaatcccag gttgtctcca cgcggctcgt ggcggatctg gagacgcccg tttcggcata
    27241 cctgaagctc accggcgctc ctggccgcga gtctcggatg agctttcttt tggaatcggt
    27301 cgagggcggg gcggttcgcg gccgttactc gatcatcggt ctggagccgg atctcgtctg
    27361 gcgcgccttc ggcgagacgg ccgaggtgaa caacaacccc atcggcgaac ccgacgcttt
    27421 cgagcgcgag cgccagccaa cgctcgccgc tcttcgcgcg ttgctcgccc gctccgccat
    27481 cgcattgccc gaggacctgc cgcccatggc tgccggtgtc ttcggataca tgggctacga
    27541 cacggtgcgg ctcatcgagc atctgcccaa cccgcgcccc gacgctctgg gggtgccgga
    27601 cgcgatcctg atgcggccga cgctcatcct catcttcgac gccgtgaagg acgagatggt
    27661 gctggtgacg cccgtgcggc cggccaaggg tgtcacggcg cgcgccgcct tcgaagcagc
    27721 cgaggcaaga ctgctgagag cggccagcgc actcgagacg ccgctgcctc atagtgctgc
    27781 gggacaaggc gcgctggaat tgccggagcc ggaatcgaac acctctcccg ccgagtacat
    27841 gagcatggtc gcgcgcgcca aggagtacat cgccgccggc gacgtgttcc aggtcgtgct
    27901 ctcccagcgg ttctccatgc catttccgtt gccgccgttt gcgctctatc gcgccctgcg
    27961 gcggacgaac ccgtcaccct tcctctatca tctcgacctc ggcagcttcg cgctcgtcgg
    28021 ctcgagcccg gagatcctcg tccgcgtccg ccgcggcgag gtgaccatcc ggccgatcgc
    28081 cggcacggcg cgccgcgggg cgaccgccgc ggaagaccag gcgctcgccg aggcgctgct
    28141 cgccgatccc aaggagcgcg ccgagcatct gatgctgctc gatctcggcc ggaacgatgt
    28201 cggccgggtg gcggagattg gctcggtgga ggtgacggaa cgcttcgtca tcgagcgcta
    28261 cagccacgtc atgcacatcg tctcgaacgt cgtcggcagg ctcgacccga agaaggacgc
    28321 catcgacgca ctgatggccg gcttcccggc cggcaccgtc tcgggcgcgc ccaaggtgcg
    28381 cgcgatggaa atcattgacg agctggagaa ggacaagcgc gggccttacg cgggctgcgt
    28441 cggctacttt tcggcggacg gtcagatgga cacctgcatc gtcctgcgta ccgcgatcgt
    28501 caaagacggc gtcatgcacg tccaggcggg cgcgggactc gtctacgatt cgattcccga
    28561 gaacgagcag caggagtgca tcaacaaagc caaggcgctc gtacgggcag ccgaggaagc
    28621 cgtacggttg gcgaatcggt ccccgcgttc gacggagcgg ggcaacggcg gccccttcgc
    28681 ctgaccttcc cgcctgggat gccggctcgg cggccggtgc gatcaagcaa gcaggtgtca
    28741 tcccggtgct cgtcaccggg atccacagct ccgcttggct tcgacgctcg ttgagaggtg
    28801 gatcccgggc atgaagcccg ggatgaccga gttctggttg gccgggtgag ttccactcgg
    28861 aggttcggga gtgcggactt acccccgatt gatgctgtcc cggagcgtct tcgagggctg
    28921 gaacttcggg acgttggctg cggggatctt gatctcctgg cccgtcgaag ggttgcggcc
    28981 cgtgcgggca gcgcgcttgg agaccttgaa cttgccgagc ggcgggatgc tcacctcctc
    29041 gcccttgctc aatgtgcttt cgatgtaaga gaccatcgca tcgaccgcgg cccccgcctt
    29101 ctccttcgag agctcggcat gccgggcgat tgcgtcgatc agatctttct tgctcatctt
    29161 cattcgtcct cgaaggtgac ccgggccgca cttttgagat caggaccctt ggccagaaca
    29221 gctgacgttg aggctacagg tgcggtgaac gaacgacttc cctcctgtgc cgtcaagtag
    29281 gaaacgcggg attcgcccga aaacacaggc gaaaatgggg atgggccttc ctctcggcat
    29341 agaaaaagat cccgccgcaa cgcgacggga tcttttcagt gtcgccactt cgcaacgctt
    29401 cagtgcgcgg tgatgcgcga ggagcccggc tgggtctcgc cgccggaaac agccgctgcc
    29461 ggctcctccc aggcgatggg ctcgggctgg cggacgagcg caaccttcag cacctcgtcc
    29521 atccgcgcga ccgggatgat ctcgagagcg ttcttgatgc tctccgggat gtccgcgagg
    29581 tccttcacgt tctcctccgg gatcagcacc ttcttgatgc cggcccgaag cgccgcgagc
    29641 agcttttcct tgaggccgcc gatcggcaac acacggccgc gcagcgtgat ctcacccgtc
    29701 atggcgacgt ccttgcggac cgcaatgccg gtcaggaccg atacgatcgc cgtgaccatc
    29761 gcgacgcctg ccgacgggcc atccttgggc gtcgcgccct cgggcacgtg cacgtggatg
    29821 tccctgcgct cgaacagcgt cggcgggatg ccgaagtcga ccgaccgcga ccggacgtag
    29881 gcgttcgccg cctggatcga ttccttcatc acgtcgcgca ggttgccggt gacggacatc
    29941 ttgcccttgc cgggcatcat gacgccctcg atggtgagga tctcgccacc gacctccgtc
    30001 catgccagac ccgtcacgac gccgacctga tcctcgagct cggcctcgcc atagcggaac
    30061 ttcggcacgc cgaggtacgt ctcgatgttc tcgggcgtca cctcgaccga cttcttctcg
    30121 gaggtcagga tctccttgac ggccttccgc gccagcttgg cgatctcgcg ctcgagatta
    30181 cgcacaccgg cttcccgcgt gtagcgccgg atgatgagct tcagtgcctc gtcgctgatc
    30241 gagaactcgc cctcctcgag cccgtgcgcc tgcttctggc ccggaatgag gtgccgcttg
    30301 gcgatctcga tcttctcatc ctcggtgtaa cccgcgatgc gaatgatctc catgcggtcc
    30361 atcagggccg gcgggatgtt gagcgtgttc gccgtcgtca cgaacatgac gttcgacaga
    30421 tcgtagtcca cctcgaggta gtgatcgttg aacgtcgagt tctgctccgg atcgagcacc
    30481 tcgagcagcg ccgccgccgg gtcaccacgg aagtccgagc ccatcttgtc gatctcgtcg
    30541 agcaggaaca gcggattgac ccgcttcgcc ttgcgcatcg actggatcac cttgccgggc
    30601 atggacccga tgtaggtgcg ccggtggccg cggatctccg cctcgtcccg cacgccgccg
    30661 agcgacatgc gcacgaactc gcggcccgtg gcattggcga tcgacttacc gagcgaggtc
    30721 ttaccaacac ctggcggacc gacgaggcac aggatcggac ccttgagctt gttggtccgg
    30781 ttctgaaccg cgagatactc gatgatccgt tccttgacct tctcgagacc gtagtgctcc
    30841 ttgtccagga tctcctgagc ctcggcgagg tccttcttca ccttaccgcg cacgccccac
    30901 gggatcgaga gcagccagtc gaggtagttg cgcaccaccg tcgcctctgc cgacatcggg
    30961 ctcatctgct tgagcttctt cagctcagca agagccttat ccttggcctc cttcgacagc
    31021 ttggtgttct cgatcttctc ctcgaactcg gcgatgtcgt cgcgctcgtc ggtgtcgccg
    31081 agctccttct ggatcgcctt catctgctcg ttgaggtagt actcgcgctg cgtcttctcc
    31141 atctggcgct tgacgcggct gcggatacgc ttctcaacct gcagaacgga gatctcgctc
    31201 tccatgagcg cgtagacgcg ctcgagccgg tccgagatga ccgtggtctc gaggatttcc
    31261 tgcttgtcgg cgatcttaat cgcgagatgc gaggcgatcg tgtcggcgag cttcgagtag
    31321 tcttcgatct ggctcaccgt gcccagcact tccggcgaga tcttcttgtt gagcttcaca
    31381 tagctctcga actgggacac cgcggagcgg gccagcgcct cgatctcatc cttcgcgccg
    31441 atcgtctcgg tgacccgctc gacctcggct tcgaagaagt cgggattgtc cgtataacgg
    31501 caaatgcgcg cacgcgtcgt gccctcgacg agcaccttga ccgtgccatc cggcagcttc
    31561 aggagctgaa gcaccgaggc cagcgtgccc atcccgtaga tggcatccgt cgatgggtcg
    31621 tcgtcacccg cattcttctg cgccgcgagc aggatgtgct tgtccgagcg catcacttcc
    31681 tcgagcgcat tgatcgactt ctcacgcccg acgaagagcg gcacgatcat gtaggggaaa
    31741 acgacgatat cgcgcagcgg cagtaccgga aacagttcct tgccggcgcc tttgtctttg
    31801 gtcgctttaa cttcgctcat ggttttctct actccctgcg cccattaccg cgtcgcgcct
    31861 cacgccccga tacgtatgga aactgttggt tcttgtactt gtcaccgccc ctacgaagat
    31921 tcgagccaat ccgtgcgcgt tggatgctgc tgcgtgaatg gccacctggc cggccggctc
    31981 ccgcactgcc tttgcggcga tcgagcgccg acctataccg acacatccgc ccgccccaga
    32041 gaaacagaca taagcgtccc cgggtcacac acaagaccat cacgccactg ccgaacaaag
    32101 ggccgcagca ggcgtcaggc gctggagccc ttttcctccg cacgctccgt atagatacgc
    32161 agcggacgtg caccgccttc gaccacctcc gggccgatca cgatttcctc gacacccttc
    32221 atgctcggca attcgaacat agtctcgagc agaatggctt ccatgatcga tctcaacccg
    32281 cgggcaccgg tcttgcgctc gatggccttg cgcgcgatcg ccctcagcgc ctcctgggtg
    32341 atagtgagct tgacgtcctc catctcgaag agccgctgat actgcttcac cagcgcgttc
    32401 ttcggctccg agaggatctg cacgagagcg tcctcgtcga ggtcctcgag cgtcgcgatg
    32461 accggcagac ggccgatgaa ctccgggatc agaccgaact tcagcagatc ctcgggctcg
    32521 acgtcccgca gcacctcgcc cgtccggcgc tcgtcagccg ctatcacccg ggcgccgaag
    32581 ccgatggaag tgcccttgcc acgccgcgat atgatcttct ccaggccggc aaaggcgccg
    32641 ccacagatga acaggatgtt ggtcgtatcc acctgcagga actcctgctg cggatgcttg
    32701 cgcccgcctt gcggcggcac gctggcgatc gtcccttcca tgatcttcag cagcgcctgc
    32761 tgcacgcctt cgccagacac gtcgcgcgtg atcgacgggt tgtccgactt gcggctgatc
    32821 ttgtcgacct cgtcgatgta gacgatgccg cgttgcgccc gctcgacgtt gtagtcggcc
    32881 gattgcagga gcttgaggat gatgttctcg acatcctcac cgacatagcc ggcctcggtc
    32941 agcgtggtgg catccgccat cgtgaacggc acatcgagta tgcgggcgag cgtctgcgcc
    33001 agcagggtct tgccgcaccc cgtcggtccg atcaggagga tgttggactt ggccagctcc
    33061 acgtcgttgt tcttcgcggc gtggttgagc cgcttgtagt ggttgtgcac ggcgacggag
    33121 agcacacgct tggcgtgatc ctggccgatc acgtaggagt cgagaaccgt cttgatttcc
    33181 tgcggtgtag gcacgccgtc gcgggacttc accagggtgt tcttgttttc ttcccggatg
    33241 atgtccatgc acagctcgac gcactcgtcg cagatgaaca ccgtcggtcc ggcgatgagc
    33301 ttgcgcacct catgctggct cttgccgcag aaggagcagt acagggtgtt cttttcttgc
    33361 tgggccattc gatcgtctca ccctcgccgg gaggcgcttg aaagttcagt tcccacgagc
    33421 atcgtgccgg tggccaaccg agcgctgaat ccatttagcc gatctgagca cgctaagtgc
    33481 atcgggatca agattggatt aacgacatca gccccgcacc atatccagtg gcgtcgtggc
    33541 cgccacacca cgacagtgtc gcaccagtgc cgatccgcaa acgatgttgg cacaacgtgg
    33601 ccgcaatacg gcccggaatg ttcgattgga atgcagctct taactatgat gcggtccgat
    33661 ctgggccgcg ccggtcgtcc ggcgcggctg ttcccctact tggcttcgag tgcctgctga
    33721 aacgcctctg gctcaggtgg ctttggcctc cgcggcggcc tcgaactcct cacgcttgag
    33781 caggacgcgg tcgacgatac caaagtcacg ggcttgctca gccgtcatga agtagtcacg
    33841 gtccagcgtc cgttcaatgg tctcgtacgg ctggccagta tgcttcacgt agatctcgtt
    33901 gagccgccgc ttcatcttga tgatgtcctc ggcgtgccgc tcgatgtcag acgcctggcc
    33961 ctggaacccg cccgaaggct ggtgcaccat gacgcgggcg ttggtcagcg cgtagcgcat
    34021 gcccttctcg ccggcgcaaa gcagcagcga gcccatcgag gccgcttgac cgatgcacag
    34081 cgtcgccacc ggcgggcgga tgaactgcat ggtgtcatag atggccatgc cggacgtcac
    34141 cacgccaccc ggcgaattga tgtacataga aatctctttc ttcgggttct ccgcctcgag
    34201 aaagagcagc tgcatgcaga tcgaagccgc ggactggtcc tcgatgggac cggtcacgaa
    34261 gatgatgcgc tccttcagca gtcgtgaggg caaatcatac ccgcgctcgc cgcgcgtcgt
    34321 ctgctcgacc accattggcc agaaggtgtt ggtgtaggta tcgatggggt cgcgcatggg
    34381 ctcgtgcctc ctctttgcgg ggccccaatt gcgggcgccc gcgtctgtcg tgtctggaaa
    34441 agaaccgatg cgttacattt agtgcggcgt acaggagttg tcgccccccg ccgacggaac
    34501 ggcctcacgc ccagctcgtt ctcaacctca agtagacagc catgcgaaag aaggcaacag
    34561 gcctggacat gcgtcggtgc cgcatcactc ctcgtccggc tcgggcttga acagctcttc
    34621 ccggtcgacc ttcttctcct cgagcttgac ctgcccgagg atgtgatcga cgaccttctc
    34681 ctcgaagatc ggcgcgcgca gctcggcgat ggcgccgggg ttcttctcgt agaactcgta
    34741 gacgagccgc tcctggcctg gatactggcg agcctgctcc atgagcgcgc gccgcatctc
    34801 gtcctgggtg acctgaatcc cgctcttctc gccaacttcg ccgaggatca gcccgaggcg
    34861 caccctgcgc tcggcgatct tgcggtactc ttccctggcc tgctcctcgg tcttgccttc
    34921 gtcctcgaag ctacggccgg cctgctcgag gccctgcgtc acctggcgcc agattgcctc
    34981 gaactcccgc tccaccaggg tcggcggcag agcgaaatcg tgccgcttct ccagctcgtc
    35041 cagcagggcc cgcttcagct tggcgcgtga agcctgggca tactcgcgct tgatctgctc
    35101 ggtcacgagt tcccgcagct tctccagatt ctcaacgccg agattcttgg cgaactcgtc
    35161 gttcacttcc ggctttttcg gaacggcgac ttccttcacc ttgacgtcga acgtcgcctc
    35221 cttgccggca agcgacttct ccggatagtt ctccgggaac gtcgccgtca cggtgcgctc
    35281 ctcgcccgcc ttggcgccct tgaggccgtc ctcgaagcca gggatgaagc caccctgccc
    35341 gagcacgatg ttgacgccct cgcccgcgcc gccctcgaac ggctcgccgc cgatcttgcc
    35401 gacgaagtcg atggtcagcc ggtcgccatc ctcggcaacc cggccctcgg tgacctcgta
    35461 gctcgtgctg cgctcgacca gctgattgat ggcctgctcg acagcactgt catcgacatc
    35521 ggccgtcagc ttctcgagct gcagctcgga aaagtcgatc aggttgatct cgggcagcac
    35581 ctcgaaggac atgccgaagg acaagtcggc ttgccccgac atcaccttct cgatttcctg
    35641 ctgatcctcc ggcaggtcga tctgcggctg gatcgccggc cgctccttgc gctcgtcgag
    35701 cgcggcgcgg ctcttctcct cgatggtctg ctgcaggacc tcgcccatga gcgaacggcc
    35761 gaacaccttt ttgaggtgct ggacgggaac cttccccttg cggaagccct tgagctgaac
    35821 cctgtccttg atctcgtcca gcctggcgtc gaacttggcg ccgatctcac ctgcgtccac
    35881 cacgaccctg agctggcgct tcaagccgtc cgaaagcgtt tccgttacct gcatgaacct
    35941 gacaacctgc ctggaatcat ccaatcccgc gtacctcgcg gacgcgcccg caccgacgtc
    36001 ggccagcgcc cgccacaaac tcaccgtctc gctgcgtggt gcgggcggag ggacttgaac
    36061 ccccacggcc gaggccacca gaacctaaat ctggcgtgtc taccagttcc accacgcccg
    36121 cagcgcaccg cgcccgtcag gcgaccaagc ctgacctgcg caagcaaccc cataatgctt
    36181 cacggcggcc gcgagggcgt gcaccggcac ggtgttccct gccacctcgc atgcatgaag
    36241 gcccgcggac atatatctgg ccaagtcccg ttagatcaat ggagaagttg gcgcgtgcga
    36301 ttcgggaggg gccgtgtccg caagggcacg atgtttcata attgacacag ggggacgcac
    36361 ctaatgctcg cacttctcgg tacacgccgc atcgctacgc tttgtgcctg cgttgtcacc
    36421 ctcctctccg cagcctccct cgccgccacc gaccgcccgg cggacacgcg cgaccacgtc
    36481 ggcatcgtcc atcgggcgcg cggccaggtc cagatcgaac gcaacggcgt cctgatcaag
    36541 gacgtaacgg ccgggcatac ccttgcccgt cgcgatcaac tctcgacggg tccgggctcc
    36601 cgcgtgatcc tctccttcaa tgacggaagc cagctggcgg tcggcgagaa cgcacagctg
    36661 gtcatcgccg acttcatgcg cgaggaaggc cgccgcagcg gcgcacttat tctcgatctc
    36721 atacgcggcg ccatccgcct cgtcgctgcc aagccgaagg aagcgccgac caagcgcgtg
    36781 gaggtgcgca cgacggcagc caccatcagc tcgcgcgggg tcgacgtctg gagcagctgc
    36841 atcggcgaca agctcgccgt cctagtgctc aagggcaagg tcgacgtgcg caacgacgcc
    36901 ggcctggtca tacttgaccg caagcggctc ggcactttcg tagcggaccg gctcagtgcg
    36961 ccggaaaagc cgagcgtctg gcccaaggcg cgcgctcagg aatcgctgct cgcggtggcg
    37021 ttcagatagc tgccgggagg cgggagtgcg ctcagctgtc gagcgcccac ataccgacga
    37081 agtagacggc gggaagaaga gcggcccaca tcagccgctc gtcggcctgg atgtatggcc
    37141 agaattggta tgcgcagagg actccggcaa caagcagtgg caagcgaaac atccgtatgg
    37201 cttcctcgcg gcaagtcagt ccgtattggg acaatctgtt ctgggacact gccccagtct
    37261 gcaatttccc tgccggccct ctgcagaaac ctgtagtggg ccctggaatg cttgcgaatt
    37321 cgttaacggc tcagcagccg agcaatgctt gcggggcgtc tgagcgggga ttaggcttta
    37381 gctcctcgac agctccgagc gagcagctaa cccggaggtt tcccttgaca gtcgcctcga
    37441 gcgttgccga cgccgttggc cacacgcccc tgatcaggtt gagacgtgca tccgaggaaa
    37501 cgggctgtac catcctcggc aaggcggagt tcatgaaccc cggccagtcg gtgaaggacc
    37561 gcgccgcgct ctatatcatc cgtgacgctg tcgcacgcgg ggcattgcgg ccaggcggca
    37621 cgatcgtgga gggcacagcg ggaaacacgg gcatcggcct gacgctcatc ggcgccgcca
    37681 tgggcttccg ttccgtcatc gtgatccccg agacccagtc acaggagaag aaggacgcgc
    37741 tgcggctgct cggcgccacg ctggtcgagg tgcccgcggt cccgtacagg aacccgaaca
    37801 actacgtgaa gtactccgcg cggctcgccg aggcgctcgc caagaccgag cccaacggcg
    37861 ccatctgggc caaccagttc gacaacgtca ccaaccgcca ggcgcatatc gaaacgacgg
    37921 ccgaggaaat ctgggagcag accgggggca aggtcgacgg ctttgcgtgc gccgtcgggt
    37981 ccggcggcac gctcggcggc gtgtcgattg ggctgaaggc caagcgcaag gacatccaga
    38041 tcggcctcgc ggacccgccg ggagcggccc tcttcagcta ctacacgacc ggcgagctga
    38101 aggccgaggg cagctcgatc acggaaggca tcgggcaagg ccgcatcacc aggaacctcg
    38161 aggatgtcga agtcgaccac gcctggctca tcgaggacgc cgaagcggtc agcatcgtct
    38221 tccagctcct ccaggaggag ggcctgtgtg tcggcccgtc ctcgggcgta aatgttgccg
    38281 gcgccatccg cttggcccgc aagatggggc cagggcatac catcgtgacg atcctggcgg
    38341 actatggcac ccgctaccag tcgaagctct tcgacccctc cttcctgcgc agcaaaaacc
    38401 tgccggtccc atcctggctg gagaaccagc ggcaggatat cccgtcggtt ttcgtcgacc
    38461 ccgagcaaac ctgaagcaaa cgcgtaacgc acatgccagc taccgagccg ctctaccgcg
    38521 ctgatcccta cctgcgtgac tgcactgcca cggtgctcgc catcaatgat cgcggcggca
    38581 tcattctgga ccgaacgatc ttctatccga ccgcaggcgg ccagcctggc gatcggggcg
    38641 ggctcgagat cgtgggctca ggctcctgcc cgatcgccac aactgtttat gatgaagacc
    38701 ggacgacgat cgtccacgtg ccggcggaag gcgcaatggc accgcagccc ggaagcagcg
    38761 ttcgtctcgt cctcgactgg gaaacacgcc accggcacat gcgcatgcac acggcgctgc
    38821 atctgctgtg cgcattggtg aagttccccg tgaccggtgg ccagatcggc gcagatgaaa
    38881 gccggctcga cttcgacatc gcggacgcca gcgccgtcga caaggaccag cttaccgagg
    38941 cgatcaacca gctcatcgcc gcagatcacc cggtcagcga gcggtggatc tctgacgagg
    39001 agctcgacgc caacccccag ctcgtgcgca ccatgagcgt caagccgccc cgcggctccg
    39061 ggcgcgtgcg tctcgttgcc atcggcgaga acgggtccgt cgatctgcag ccctgcggag
    39121 ggacccacgt caggtcgact ggcgagatcg ggaggatcgt ggtcagccgc atcgagaaaa
    39181 agggcaagca gaaccgccgc atccggctgg cgtttgcttg agaccgccga ctacttaagg
    39241 aggcaatcga aacatggcac ccgtaagcgg ctcctatgtc gtcgagaccg actggcttgc
    39301 ggctcatctg aacgcccccg acctggtcgt cctcgacggg tcctggcatc tgccgacagc
    39361 gggccgtgat gcgtgcgcgg agtacctcgc cgagcatatt ccgggcgctc tgttcttcga
    39421 tatcgacgaa atctccgacg agacctcacc gctgccgcac atgctgccct cgacggtgaa
    39481 gttcgcgagc cgcatgaaga agatggggat cggcgacggc atgcgcgtcg tcgtctacga
    39541 cagcgtcggc atgttttcgg ccgcccgcgt ctggtggacg tttcgtgtca tgggtcacga
    39601 ggacgtcgcc gtgctgaatg gtggtctgcc gaagtggaag gcagaagggc gcccggtcac
    39661 ctcggagcct ccgccggccc gctccgagcg gcatttcacg ccccggctca acgccagcct
    39721 cgtgcgtgac ctggaggaga tgaagcgtat cgtcgaaagt ggctcggcgc agatcgcgga
    39781 tgcgcgcgga ccaggtcgtt tcaccggtac ggagccggag ccccggcccg gcttgcggag
    39841 cggccatata cccggctctc gcaacgtgcc ctattcgcgc ctctgcaacc ccgacggcac
    39901 actgaagcct gctccggagc tgcaccggct gttcagcgaa gccgggatcg atccttcaaa
    39961 gcccgtcgtg acgacgtgcg ggtccggcgt ttccgcagcc gtgctgagcc tggcccttgc
    40021 catcatcggc cagcccgatt ccgcgctcta cgatggctcc tggagcgaat ggggccggga
    40081 cgacgcaggc acgccggtgg aaacgggctg agtatcagtt tgatggagct ggtcgagctg
    40141 tatggggcgc cggggtcagt cgtcgtgtga agcgtccgga cggtgctgca ggaccagcgg
    40201 cgaaatcaca tcgatgcggt ctgtccagat accgccggcg tacccgtcgg gcagtgattg
    40261 cagctcctcg cgcgtattga ccccggtcga gaactctccg ccgcgataag gcccgacggc
    40321 gtagattttg gtcccgtacc gctcgagccg tcgctggaag acgtgaggcc agccccagag
    40381 ccagggtcca atgttcgtcg gcacgagcaa cagcgtgcct cggcaggctt ccggaacata
    40441 gcccgcccaa ccggtcagtg cgtatcggat gaaacaatcc tgcagggtgc ggcgggacat
    40501 gacccgcaag gccggaagct cctggcgtaa gactgcgatc ggccgatccc caccatacac
    40561 catgagctgc tcgagccgct cacttgggag cacggagagc cgcgccgcca gaagccgccc
    40621 ttcccccgga tcattgctct tcacgttgat gagcagcggc ttgtccggaa acgcggcgag
    40681 cacctcgtcc agcgagggca tcaacccgac gcctttgccc cggaacggga aggttttccc
    40741 gcgatctgcc gaatagccgt agccgacgtc caaagccttc aactgcgcca ggctgtgctc
    40801 gcgcgtaacc ccgtggccgt tggtacggca atcgagtgtc cagtcgtgga agacggcaaa
    40861 gtgcccgtcc gtcgtcgggt gaatgtccag ctccaccaca tccgcgccga gttcgaaggc
    40921 ggcgcgcatg gaggcaatgg tgttctcgag gtaatcgtgg accggcggat cgatgcgcgt
    40981 tgcggtgcac gtttctcccg taagaccggt tttgtcatac gtctgcgcaa gtccgcgatg
    41041 cgcgagaagc gacggccgat ccgccggggg ctgcgcgaga taacttgagt tgtttaggta
    41101 aacggcggcc gccgcgaccg ccgctatccc caaaagcatg acgagccgca ttggcgttga
    41161 ccgcaatagt catgaacaag agtggaggag gggcgcggtc ccggataagc cgatccggtc
    41221 acggcattcc gacgcgtggc ccccgcggct ggtcaaaacg gatggtattg gtagagaacc
    41281 gtttctgaca gtgcgtgccc atcaaggcgc aggtagagcc tgatttcgac gggctccgac
    41341 ccttccgcag cgaggtcgaa ttgcgcgcgc cagtggccgg ggacgtcgtt gggcaccgcc
    41401 tccgtgaaga cgtgagatgt ctcgccccgg gaaaccgtaa ccaccgcttc cggcagcgtg
    41461 ccgttcggca gcttggcaag cggctcgccc agaaactcga ccacgaactt tctgcgcccc
    41521 tcgggccggg gctttccggg ctccccacct ctgccgattc ggagcgccac cgcccgtgcg
    41581 agcgggcttg ggttgggctc ctcggcctgc cagtagagcc tgtaccggta gctgagcgac
    41641 tgccccgcct ttgccggctc cgctgggacc caggccgcaa cgatgttgtc gtggatctcg
    41701 tcgtcggtcg ggatctcgat gagttgcacc gtccctttgc cccagtcgcc gagaggctcc
    41761 acccacagtg acgggcggcg ttcataggct acgccatcaa gataatgatc ggtgactcta
    41821 tcccgctgca tcaacccgaa accccgaaca ttctcgtcgg agaaggccga ggtcacggtc
    41881 cggggcggat tgttgagcgg ccgccagatg cgctcgccag cccccgtcca catggccagt
    41941 ccgtccgaat cgtggatctc ggggcgccaa tcgatcgcgg cgggcttggc cgtttcggaa
    42001 taccaataca tggacgtcaa aggagcgatg caaagccgct gaatatcgcg gcgcaggtgg
    42061 atttccttct cgatatccat cgttacggcc ctgtcgcgcc gcatgcggaa gcgaaacgca
    42121 cccgttacgc tcggcccctc cagcaaggcg tagacggtga cctcgttcga cccgtcggcg
    42181 ggcgtctcga tgtagaagtt cgtgaagtcg ggaaattcct cggcaacacc cgccaccgcc
    42241 gggtctaccg ccaggccgcg tgccgagatg ccgtactggt aatccgagcc gatggcgcgg
    42301 aaataggagg ctccgaggaa agcgacccaa tcgttcttcc gccaatcgag cgtttcaccg
    42361 cgccgcccag gatggccaaa tcggctctcc tggaagcgga aacccgcaaa gcccgcattt
    42421 tcggggagct gtcgggcggg cgagtctgcg ggcatgtcga agtactcggg gcgatagatg
    42481 atctccttcg cctcccctgc gatgaccacg tgcatgcgca ccgtcttggg gaagttctgg
    42541 cccagatgga agaacgtcac cggaaagcgg ccggggccgt tggcaaacag ggcgtaatcc
    42601 ggcttgaacc ggatggcccc gtgcgcctca tagtcgatct gttggacgat ttcaggagcc
    42661 ggacgcggcg gcggaacata cgggcctttc gccaacgtac gtgcccgctc cttcagccat
    42721 tcgtaggaaa acggaaaacc cgtcccgacg atgagacccg acgcgccttc tgcccacgcc
    42781 tgccgagcca atagcgccac tgagacgagg cctccagctc ccgacagcac ctgccgacgg
    42841 ctcacgtagg tgatcttgcc agtttttgaa ttcatgaggc tcatggtttc cggcgtgctc
    42901 gttcggattt taaggtgaat ggggcgcgca gtcctaacgg tcggcttgcg gcagccgtgt
    42961 ggcaaggccg ggtcggaact gtcgcggccc cgccggcagg tgaggaaaac tccgcttctt
    43021 cccgggcggc gacctgggct aaccttcgtg cgcggttata cttggtccgg cccgggatgc
    43081 ggagcggctc cccggacatc agacaacata tggggaagct atggcaggcg ttctaatcgt
    43141 gacaggtggc agccgcggca tcggcgctgc aacagcaaag ctgggggcgg cgcgcggcta
    43201 tcgggtcgct gtcaactaca atcgcaatcc ggccagcgcc gaagctgtcg tcaaggagat
    43261 cgagcagtcc ggcgggcgcg caattgccat ccaggccgac gtcagccgcg aagccgacgt
    43321 cgtgcggctg ttcgagaccg tcgaccgaga gctcggccgg gtgtccgccc tcttcaacaa
    43381 tgccggcgtg atacaccgga tgaagccgat caccgacttc gccgaggcgg aactggccga
    43441 gatgtggcgg gtcaacatca ccagccagtt cctctgcgcg cgcgaggccg caaaacgcat
    43501 gtccacaaag ctcggcggtg agggcggagc catcgtgaac atgtcctcgg cagcggctcg
    43561 cctgggcgga gccaacgggt cgctcgccta tgccgcatcc aaaggggcga tcgacacgtt
    43621 caccaccggg ctggcgctcg agctcgcgcc gcagggcatt cgtgtgactg gcgtgcggcc
    43681 cggtctgatc gacaccacga tccacaacga catgggcgat ccggaccgat tggcgaagct
    43741 ccggccgacc gtgccgctgg gccgcacagg ccgggtcgaa gaagttgccg aagccgtgct
    43801 gtggctattg tcgccacagg cgtcctacgt cacgaacacc atcatcgata tcggcggagg
    43861 ccgctgacgg gacacttccg tgttggacct tttctgcctt cgccgcgttt ctgggcagat
    43921 gatctagtcc cgctctgcag ggttgcgatg cgccgctgca tcagctccag ttcggacgcg
    43981 gccccatgat ccggaccagg acttgggtga ggcgctctct gatcggtgcc ggactcgttc
    44041 tggcagccgc agccggcgcg accgctgtcg tcgccatcgg cactgttccc ccactgacgg
    44101 gtgagctcgc cgtgcgcggg ctttccaagc cggtctccat cgtgcgcgat cgttacgccg
    44161 tgccacacat cacgggcgcg actctggccg atgtctaccg cggcatcggc ttcgcgcatg
    44221 cccaggaccg cctctggcag atggaggtga tgcgccgcgc cgggcaaggc cggctgtccg
    44281 agatcttcgg cgagcggacc gccgacgccg acattttcct gcggaccctt gatctcgccg
    44341 gaaccgcgga acgcagcttc gccagatttc cgcgcgaggc gcaggagctg ctcaaagctt
    44401 atgccgaggg cgtgaacgcc tttatcgagc ggccgacggg gctgctggag cagcgcttgc
    44461 cgcccgagtt tctgctactc ggctaccggc ccgagccttg gcggcctgcc gatagcctga
    44521 ccatcgtcaa gatgatggcg ctccacctca gcaccaatct cggtgcggag atccagcggc
    44581 tcaccctggc ggcgcaaggt ttttccccgg cggagatcga cgacgtgatg ccgcttaacg
    44641 acggcgagaa cccgcctccc cttcccgagc ttgcgacgct ctatccgctg cgaagcctgg
    44701 agaagacgac cccgcgctca cagaccgccg cgctcgatgt gttcctcgga gccggggcct
    44761 ccaacaactg ggtggtcgcg ggctcacgga cggtgacggg gaaaccactg ctcgccaacg
    44821 acccgcacct gcggctgtca gcgccgtcga tctggtatct ggcccacctc gcgctcgacc
    44881 gccctgggcg tgagcgaata gacgttgtcg gtgccagcct gccgggaaca cccgtcgtgg
    44941 tgctcggacg cagcaatacg ctcgcctggg gcttcaccaa caccgaagcc gacgtgcagg
    45001 acctgttcat cgaaaagacg aatccggacg atcccgagtt gtacctcacg ccggacggct
    45061 ggcgaaggtt cgacgttgac gtgatggaga tccgggtccg cgggggcgaa acgcgcctcg
    45121 tggagcggcg ccgaacccgg cacggcccgg tgctgccagc gagctttaga aacctgggcc
    45181 agatccttgg gccaggccac gtcgcagctt tgcagtggac cgccttgtcg gacgacgaca
    45241 cgacgatcct cgccgggctg ctcgacgaga acgcccgaac gatttttgac tatatggagc
    45301 ggtcacgttg gtacgtggtc cccatgcaga acatggtcgt tgctgacact gaagggcata
    45361 tcggcatgat cgtgccgggg cgtctgccga tgcgccaccc cgacaacaag gtagccggtc
    45421 gcgccccggt ccccggttgg gaggcggtct acgactggca gggcttcgtc ccgttcgatg
    45481 agctcccccg taccgttgat ccgccggaag gtgcgatcgg cacggccaat gcgcgcatcg
    45541 ctgacggctc ctacccgcac ctcatcacct atgattggga agctgattat cgcagccgtc
    45601 gcgtcaacga gctgatcaac ggcaaaacgg ggcatgacct cgagacgatg cgggcggcgc
    45661 agctcgacgt tttctcaccc gcttttgccg agttggcgcc attgatgatc gctgcggcca
    45721 ggccctttgt ttcgtcgccg aatgggcagg atattctcga ccggctcagc cgctgggatg
    45781 ccttcatgcg agcggatgct gtcgagccgc tcctcttcat ggcctggctg agacagtcgg
    45841 tgcgcgccat ctatgccgat gatttcggtg cggctttcga ccaattctat catccgcggg
    45901 cgaaggcgat gatccgcctg ctcagagggg aaggcagccg cgattggtgc aattcaagga
    45961 gtacaaagaa aaccgaaact tgcggagaag tccttgcggc ggccctgaag cgcgccatcg
    46021 aggagctcga agccaaattc agctccagcc gcgcgggctg gtcatggggt aaggcgcatg
    46081 tcgcgactgg agagaaccag gtcttcggcc aactgccttt cgtcggccgc ttcttccaga
    46141 tcgggctggc atcgccgggc ggcccctata cgctcaaccg aggcgtgacg gagttcggca
    46201 gcgatgcgcc gttcgccaat cgccacgcat caacctatag ggcgatctac gatctttcga
    46261 accttgacgg atcgcttttc atgcagccga ccggacagtc cggcaatccg ttttcacggt
    46321 attaccggac tttcgagaaa agttgggcgg agggcgacta cattcaaatc ccgacggatc
    46381 ccgctgcgat cgagcgtgat gccatcggaa ggtggctgct cacgccatca gcggatggcg
    46441 ccggctagat catttccgat atgaatgaaa acaagccacg cttgcgccaa acctccctct
    46501 ccccggcggg gagagggtcg gggtgagggg ttccgctagt ggcaactgtt acactcccct
    46561 tcgggccgac ctctccccat ggaggcaaaa ggcccaacta ctgctgccgc gttccggaaa
    46621 aactatagat ccacctgcgc gtcagaccgg cgacgtgctc cgcattaatt tcgtcgcaaa
    46681 catcaccagg aacagaatgg cgcttgcgag caggagcaga gacccgatga tcgcgagagc
    46741 cggctggcca tggaattggg cgagcggcag gccgatcaga aacaccggcg ctccgcaaac
    46801 cgcaagcaga aactgagggg tggaaagaaa tgaatcgcgc aaggccggcc aaagccgatg
    46861 cgtgatcccg tatagcgcat gggcgacgaa ccccaccaga tttatgtgcg cgtggaggtg
    46921 ggcataagcg aagtcctccg caatacccat accgatcccc agccccatgc cggccacgaa
    46981 gtaaaggaat gcaataatca ggaagatcga gtcgatatga gctttcatgg cgtcagcgcc
    47041 tgcccgaatg aagagaaaac tgacaatctg tcctggtcat tccttttgtc aactgctttg
    47101 gcaggcgcgg gggcagaaaa actcctgtga ggatttgtgg ggagggctcg caatgccaaa
    47161 tgcgggcgag ccacgaatgg aacgttgccc gactgcgaca gccagcggca accatcaaca
    47221 gctccctcca gaatgggttg tcaaacgatc caagatggct ttgctcgggt ctagaatgaa
    47281 gtccaccata cgtcttgggg agggggtcat ccatgcggaa gcgctttttt tggatgcttc
    47341 tggcgggaac gatgctggcg gccgggggac acgcctttgc agtggccacg tctggcagcg
    47401 agagcagcgg ccgcaccgag gcgcccgcga gcgtacgcgt cgaccaatgg ccgaaatcga
    47461 tcgacgagtt cctggcggga aatcaccttg aacgctcaga cgtggttctg acgcgccgcg
    47521 agtgggatcc tgcctcctat ctgatccgct gggccaccaa atcgccgttc tcccattcgg
    47581 ccctgatctt cacgagcccc attcacgagt ccggctacaa aagcacgttc gtgatcgaag
    47641 ccaccactga tggcgtcgac ctcagcaatt tccgcgatta cctcgaagac aagagcgcca
    47701 tggtggcgat caagcgcctc cggcgtgact ggttcgacgc agacaagcag agccgggtgc
    47761 gcggccttct gctcgagaac attaaggccg actataatta ctgggcaatc gtcaggattg
    47821 ttcgtgacct gtggttcggc atccagcaca ccatcgaggg acaggagcag accgtcgaat
    47881 cgttccgcga cagcgactgg aagccgccga actcctacat ttgctccggc ctggtccaag
    47941 tgggattcgt cgaggcaatt tcggaatatg tgctgcgacg gcagcttccg ccctgggccc
    48001 tgaacgaggt cgtcttcaat ccctctgccg ccagccgcct gccgccaccg gaggcctgga
    48061 aagaggcgcc gccgtcgctc atcgacgacg tgatcccgca attccgcgtc cagctcgcca
    48121 ccgagctcga agctgtcacg ccggaggacc ttgccaggag cgagaagctc gaatggctct
    48181 atttcatccg ggatggtaag gtccacaagg tcagctccta cgacgaggtc ctggagctga
    48241 tgaaatagat cacggcggcg atacaaaaaa ttcagcgcat cgccggatag cgccagaaag
    48301 caccggctga cgtcgtgacg cgctccagaa tggggatgcg cctttcaggc ggccgttctc
    48361 ccgtctggat caggagccgt atgtcattca gcagctccgt cttttcggcg tagcccgaat
    48421 ggttgagcga gaacatctcg gtgttgatcg ccgtcacgtc gatcgtgtcg acaccaggca
    48481 cgatgatggg gccttctggc ggaacatcgc cggcgcgggg gacgccgccc cagaactggc
    48541 gcgaggcggc aagcgcccga tcgttggcag ccgcgaacag cgtgaccccg ccgctgatgc
    48601 cctggatctc gcgcgccaga aactcgaacg aatcgcgatc cacgtctggc gctgcaagga
    48661 tcacctgcga aatcttcacg ccctccggcg cggaccggcg catgtcacgc agcacaggca
    48721 gcaggagctg attgcccatg ctgtgcgcga tgacgctgac cgactttgca ccggtctccc
    48781 tgacgacaag gtccagaaat tcacggaaat agggctccgc cgctccggcg ctctcccgat
    48841 cgtagctgta gtcctgtacg cccaaggccc ctttcgatgg ccaactgtaa acgaatggcg
    48901 cgccatcgaa cttcaggtcg taggccatct gcgccgtacg aaacacggcg aactcgaagc
    48961 tcgtattgaa gccgtgcacg aacacgagcg cgtggtcctt gtaggcgacc gattcagcca
    49021 gtcgcttgcg gacgagctcg aggaactcga ggctgccaag ctcgcgaatt tccttgatcg
    49081 tgaagtgctt tttcgggtcc tcctcctcct gccagatgac gattttggtg aaaggaatgt
    49141 gatagaccca aggccgttcg atctccggca cctgatggga tttgggcaca gtcaccaggg
    49201 catggcccag ctgcaggcgc ttgccgcgct ccgccccgta atcaactcgt ttgccgccgg
    49261 aaagcttggc gcggtcggtg ccatagaata cggggaccac atcccaatcg ctctcctcgg
    49321 gcggaggcgt tgcaccggcc gccggcggag cttcggcgct tcgccgggct ggcgcccctg
    49381 agtcggttgt gggcgccggg ggcgccgatt ccgcaaacgg acgctcgcca tcatcagaca
    49441 agggcgatcc tgccgggtcc cgcgctgccg gggctccatc tttgggagcc gcggacctct
    49501 ttgaggaacc gatccggcgc gaacgcgcca gatcctcggg cgccgtgcca aactcccgtc
    49561 catgcgtctg ccattcggct gccggccgct cctgatcatc agcgcctccg acttcctgtt
    49621 cgacacgtcc ttcgggagcg gctgacttat caggagagcc catctgacca agttgaagtc
    49681 cgacggccag ggggagcaac atcatcaacg caagggccga gcggacgatg atcctggtca
    49741 gaagcccttt tcgccgcgtc agccaccatg cgatcgcgta aaggaagagc gcaacgccgg
    49801 cgagcagcac atagatcgcg ttctgcgata actccatgga acccctcccc acttcttttt
    49861 ctcggcggcg cactctagga gcgcgaccgg gccgccgcaa ccgatctcga tcatcaggac
    49921 gctgtgtatt ttgataacta tcgtggcatc ggttgggcgt gcagcgatgc ttccaaggga
    49981 cttgcctctc gtgcccatcg cggccaagtt cgcatcgcgt atcgggagca gaggatgaaa
    50041 agcagcccta agcgcacaga gttgagcctg tccgggctgg tcgacggcat ccgccgcggt
    50101 gaccgggccg tgctggcgcg ggcgatcacg ctcgtcgaga gcaccaggcc ggacgaccag
    50161 aagctggcgc aggacctgct gcaggagctc ttgcccgcaa gtggcggggc atggcgcatc
    50221 ggcatcaccg gcactcccgg cgtgggcaaa tcgacaacca tcgatcagct cggcacgaac
    50281 ctgatcgctg ccggacacag ggttgccgtg ctcgccgtcg acccgacctc gcggcgctcg
    50341 ggcgggtcca tcctcggcga caagacccgg atggcgcgcc ttgccgcgag ccccaacgcc
    50401 ttcattcgcc cgtccccctc ctccggcacg ctcggcggcg tcacgcggcg gacgcgcgag
    50461 acgatgctgc tggtcgaggc cgcgggcttc gacgtcgtca tcgtagagac ggtaggcgtc
    50521 ggccagtcgg aaacggccgt ggccgatatg gtcgatttct tcctcgtgct gatgctggct
    50581 ggcggcggcg acgagttgca gggtatcaaa aagggcatta tcgagatcgc cgacatgatc
    50641 gtcatcaaca aggccgatgg cgataacatc gagcgatcga aacgcaccgc cgctgattac
    50701 cgggcggcgc tcaatattct cacaccgcgc acggcaacat ggaaaccgcc ggtgctcact
    50761 gcctcagcgg ttgaaaatcg cggcctcgac acggtgtggg ccaagatcgc cgagcaccgg
    50821 aggcggatga cagacagcgg ggagctccag gcgaagcgtc aggagcaggc cgtcaactgg
    50881 atgcgcgaca tgctccagga acgcctgatg gaagcgctga agtcgcgcga tcacgcggca
    50941 cagcggattg cggagctgga ggaggaagtc cgtcacggcc ggatgacggc gccgcgggcg
    51001 gtcgacgaag ctcttcgtct cctcgagaag tgaccagtcc ggcatggact ggccacctct
    51061 tg
//
LOCUS       DASVDU010000181        12209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1178726, whole genome shotgun sequence.
ACCESSION   DASVDU010000181 DASVDU010000000
VERSION     DASVDU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12209
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178726"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VF226_11845"
     CDS             complement(<1..688)
                     /locus_tag="VF226_11845"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7074726.1"
                     /translation="MDAADTNPESAQDRDGGGLGRRTFLKGASMVGGSLITAGLVAGP
                     QATLARNLSEGAAAAAATSAASSRRAGSAALTPKEKEVLDLIDENEVASVLQDFVRIH
                     TDEANEQAGAAYFARRLREWGIPDVMIQGIEDHPGRSNLIARVPGRTPKGPTLMITSH
                     LDSIPVVDTADRKDWFVDPFGGVIRDGRVYGRGAADNKSGCVAKIMATKAIKEAGFDL
                     PGDLLTVLVAD"
     gene            1950..3677
                     /locus_tag="VF226_11850"
     CDS             1950..3677
                     /locus_tag="VF226_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7074727.1"
                     /translation="MADVVTKDGVPSSPVTYYEVGVRKIPGLDPLTMDASVSVLSTWN
                     TIEYTAGVNGRRFTFGDYGTEFYYRDGSIPAEKHHESSLWQGSRANANLNASTEIGST
                     SARVNTDFEVNDLFRSTLAVEAPGSTRDGDGVDLFVYQKDMRQFELGADFEHAFGNNF
                     SGKAIVLYNLEETITAQSQLNVENGADAVYRRSDSNLRTTEAISRWELNWLGWPGHAL
                     RFNVEAARNVIGSALVQEVDSGFGLQEVSVPGSNTRVQEVRGELLIGDTWRLEEYVLS
                     YGVGAEASTISQSGDSVMTRDFLFFKPELQLTYSQSQDWQTRLRIAREVAQLNLNDFV
                     SAPVFQDDDLELGNPELEPESRWVAELSGERRFGSLGVIRGTLFYNWLSEVQDLVPLT
                     PVFEAPGNIGDGRRWGLVVEATLPLSQIGIPAARLDIEAHLEDSAVTDPVTGHERVLS
                     GEAVSGRPISFGEGERYGYALEFRQDFSDAQIAWGLDMRSRAERTSFKVNELAEYSEG
                     AEVNAFIQTTRLFGQRIRLSAENLLNMQVLRTRTLFIGLRALSPVNVIEQRDFQNQRR
                     IILSISGSF"
     gene            complement(3781..4008)
                     /locus_tag="VF226_11855"
     CDS             complement(3781..4008)
                     /locus_tag="VF226_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074728.1"
                     /translation="MQPQPNLLDVDHIEEAAALVAPGARPERGRRARVQLAPLAGEGT
                     PDHERVQPQRLDLDRLADMRGTRTTVDFGVH"
     gene            4227..6884
                     /locus_tag="VF226_11860"
     CDS             4227..6884
                     /locus_tag="VF226_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX7074729.1"
                     /translation="MPGSLNTPVGAERSRLAEDSRGAVRWKLWGPYLSERQWGTVRED
                     YSPHGTAWDYFPHDHARSRAYRWGEDGIAGLSDEQQLLCFALALWNGKDPILKERLFG
                     LTNAEGNHGEDVKELYYYLDATPTHSYLKYLYKYPQAAFPYARLVEENRRRGRNDPEF
                     ELIDTGVFEADEYFDVFVEYAKAGPEDVLVRITAHNRGPQAAALHLLPQLWFRNTWSW
                     SANAPRPEMHIAADGSVAALHAELGEYRLYADGRPDVLFTDNDSNRERLFGVSGDRGH
                     WKDAFHEYVVHGRDAAVNVECRGTKAALHHRLRIAAGETAQIRLRLRRGPAAVPFEEF
                     ELSFGQRVAEADGYYAALQSGLPSEDARSIQRQAFAGLIWSKQFYFYDIAQWLDGDPA
                     EPPPPQSRHHGRNADWRHLNNRDILSLPDKWEYPWYAAWDLAFHCIPLALLDAAFAKR
                     QLVTLARAWYMHPNGQLPAYEWDFSHVNPPVHAWAAWQVFQIDRKNRSDQGDLAFLER
                     ILHKLMLNFTWWVNRKDAQGRNIFQGGFLGLDNIGVFDRSAPLPTGGHINQADGTAWM
                     AMYCLNLMRIALELARHNRVYEDIATKFFEHFLYIAEAMTNMAGAGIGLWNAEDQFFY
                     DELSLPDGRLVPLKVRSIVGLIPLFAVETLEPELLDELPEFRRRLEWFLNYRPDLTQL
                     VSRMEKPGVGERRLLSLLRADRVQQILRRVLDQAEFLSDFGIRALSKYHEQHPFVFEH
                     GGERLSVSYQPGESGSGVFGGNSNWRGPVWFPVNYLLIESLQKFHHYYGDEVGIECPT
                     GSGSRLTLEEVAEELTLRLSRIFLRDAAGRRAVFGENSKLQNDPDFRDYVPFYEYFHG
                     DNGRGAGASHQTGWTGLIARLLQPSAYER"
     gene            complement(7142..8305)
                     /locus_tag="VF226_11865"
     CDS             complement(7142..8305)
                     /locus_tag="VF226_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074730.1"
                     /translation="MRKLGLLIGLAFVGALAIAAGQQPISVEERLIRIQVEEALPEYW
                     GVLKGEPIDVQAVLLDYADDPILLLKAKVALLKYPELAREVLPLYGSEAGFQEILGNY
                     GEGVLLPVHYFLNNEISTVAFMHYSARKLRETQDAAKRLWHGTGSELESEGEGAIPKA
                     GSAEQGSSLTREQRGRYAVNFIRDEGHDFLGQFTTDGEGNVRWIQTERVLEGANAFFA
                     SGIRNLETRLRTDQDIGAGDVGWAAVDAVVVGSAVKLLRMGRATAASTRYAGFSARTT
                     AYASRVGRAGQMAVRIGRYARWPAAVAGVYLVVSRPGIISDVLAGIADVLGWPVWPAQ
                     ALGWMLILLPVLWIVSVLARVILRPAIRLLRTLVQVLVWADRQVNSAAAKPAR"
     gene            complement(8326..8583)
                     /locus_tag="VF226_11870"
     CDS             complement(8326..8583)
                     /locus_tag="VF226_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7074731.1"
                     /translation="MRGLRRTVFHAGNHRAEAAGDHQVSGRITSILNGKRAITPETAL
                     RLSRYFGNSAQFWMNLQTRYDLREAERKLGKRIEAEVGRVA"
     gene            8741..9907
                     /locus_tag="VF226_11875"
     CDS             8741..9907
                     /locus_tag="VF226_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074732.1"
                     /translation="MRRLEDDAMPAPLEACSPTPRTGTPRRWWPALLLVCLLPAPLLA
                     QDDDAAQAGVGFAQIEVTDPIRGGQMPGYVFYPSPARAGSPTRVGPYEVEGVRDAPAR
                     PGTRPMVLLSHGHAGSNLGHHDLATYLAGHGFVVAAIAHSGDDYRDASFDGHPEVLGG
                     RPVQVSATIDALLRDPRWKPLIDADRIGVAGFSNGGYTSLLLVGAVPSLRRFVDYCRR
                     YPGDRLCAGAKDVEAEAERQGQTPEQYMDAMQARLTHWGKTSDPRVKAAFAMAPLSLV
                     FDADGLAGIDRPVFLYYSERDTVLRPSENAARIRPLLKTLAGVRTVPRADHWVFIPPC
                     TRELAEVVAEICSDPPGVDRARTHARINADALAFFRKTLAPTTDAPAATKSGQP"
     gene            complement(10276..11613)
                     /gene="ltrA"
                     /locus_tag="VF226_11880"
     CDS             complement(10276..11613)
                     /gene="ltrA"
                     /locus_tag="VF226_11880"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX7074733.1"
                     /translation="MTRTSSREIISTRQEKLATLARIEPKLELSTLAHHIDEVWLREA
                     YRRTRKDGAVGVDGVTAEAYEAHLEANLSSLLGRFKSGRYRAPAVRRVHIPKDGTRKK
                     TRPIGIPTLEDKVLQRAVLMVLEPVYEQDFLSCSYGFRPGRSAHQALQALWQGLMEIG
                     GGWIIDLDIQSFFDDVDRTHLRHFMGQRVRDGVIRRAIGKWLNAGVMEEGVVRHPERG
                     TPQGGVISPLLSNLYLHEVLDMWFEQEVKPRLRGRAMEIRFADDAVLAFEREEDARRV
                     LAVLPKRFGKYGLTLHPEKTRLVDFCRPPGSGEGSQRERSFELLGFTHFWGRSRQGRW
                     VVRRKTAKARFSRALKRVTAWCRTHRHLPVAEQQMALSRKLQGHYAYYGITGNATALL
                     RFRYEVERCWRKWLSRRSRKARLNWAQFDRLRRRYPLPPARVVHSIYRGAATS"
BASE COUNT         2336 a         3923 c         3607 g         2343 t
ORIGIN      
        1 cgtcggccac cagcacggtc aagaggtccc ccggcaaatc gaaccccgcc tccttgattg
       61 ccttcgtggc catgatcttc gccacgcagc ccgacttgtt gtcggccgct ccgcggccat
      121 agacgcggcc gtcccggatt acgccgccga acggatccac gaaccagtcc ttgcgatcgg
      181 ccgtgtccac cacggggatg ctgtccaagt gagaggtgat catcagggtc ggacccttgg
      241 gcgtccgccc tggaaccctg gcgatcaggt tggaccggcc aggatggtct tcgataccct
      301 gaatcatgac gtcgggtatg ccccattcac gcagccgtct ggcaaagtac gccgcgccgg
      361 cctgctcatt ggcctcgtcg gtgtgaatgc gtacgaaatc ctgcaggacc gaagccactt
      421 cgttctcgtc gatcaggtcg aggacttcct tttccttggg agtgagcgca gcggagccgg
      481 ctctccggga tgacgcggcg gacgttgcgg ccgctgcagc ggccccttcc gacaagttcc
      541 tggccaaggt tgcctgcgga ccggccacca gccctgcggt tatcagcgag ccgcccacca
      601 tcgacgctcc cttgaggaac gtccgtcgac cgaggccccc gccatctctg tcctgtgcac
      661 tttctggatt cgtatcggct gcgtccatgg tgtcgtcccc cttatcagaa cgtcattgcc
      721 agctcaaatg cgtattcact ccatctcctg cagaaacaca tatcttcgtg ctatgcgccg
      781 atcctttgcg tttgcgcctc caccatggcc gatccgaaca gcggcgacat cggatcctcg
      841 cggtgagcac tcgcatggcc tgcgacttat gacgaaaggc ccctaatctc gtctcggatg
      901 aacaactgct ctttgctggc ctcacggccg tcacgcgggg ctgcatcacc tcggaagcga
      961 gatgacacaa ccgatcagag cggcctggaa tcctcgactg ccgatcagca acagtcgatc
     1021 gaacattcac gagcactatc gttgtacatg ccctgcccca gtacgacctg cggtaaacct
     1081 cagttgccgt ggtgagcgat ctgttcatca tgatgttaca tagacaatgc gccaagggtt
     1141 agcgccaact cgtttcaacg cataggacca gcgcgtcctc ctccgctttc gacggctaga
     1201 atgacgcact gagtcacgcg cggcatcggg gactcgccgc cgataaaatg cggtgccggt
     1261 ttcgaccagt ttgctccggc ctgcaaggga cggcgtttct ccgacgcagc gcgctcccat
     1321 atgcggcaca tggatcacgc ggcgccatcg gcagagcaca agagttgcac aaactgcacc
     1381 gttccggagc gcagcgccgc cgtgcggccc cgtatctcaa cctggcgaga gttcaaagat
     1441 ggttgaatac agaagacggg cggaactcaa aaaagcatca ccggcaaaat gcggcacaaa
     1501 gctactttgt ttgcgtcgcg atgcgggcca acacctcaca gcatcgaggc ggacttcttg
     1561 ttcatacaca ccacttgcat gcctggagtt gatggtcggc tacttgagca ctattccctt
     1621 accgctcttg gctcagaaaa tgggagcggc ggcgatgcta ccgatagaaa ccacaccggt
     1681 agatgacgtc atctacggcc ccgacttttt tgaccagtat gagctgatca cagccctgga
     1741 gatggtgccc tggttttcgg ctcgacgagg gtggcgaggc acgcggacta ggcggtgcaa
     1801 ccgggaatgt tttggtagac ggtcaatacc caagctcaaa acaggacacg ctttcacaac
     1861 tattgggccg gatcccagcg agccaggtcg atcacatagt agtgatcaga agtcgggcca
     1921 gcggcataga tttgcgagat cactccgccg tggcagacgt cgtcacgaag gatggggtgc
     1981 cgtcatcgcc tgtaacgtac tatgaagttg gcgtccgaaa aatccccgga ctcgatccgc
     2041 tcactatgga tgcgagcgtc tccgtcttga gtacctggaa caccatcgaa tacacggccg
     2101 gcgtgaatgg gcggcgcttc acctttggcg actacggcac ggaattctac taccgagatg
     2161 gcagtattcc agcggagaag caccatgaga gctcgttgtg gcaaggcagc agggcgaacg
     2221 ctaatctgaa tgcgtcaacc gaaattggca gcacctcggc cagagtaaac acagattttg
     2281 aggtcaatga cctctttcga tctactcttg ccgttgaggc accagggagt acacgtgacg
     2341 gcgatggcgt ggacctcttc gtctaccaga aggacatgcg tcagttcgag ctcggtgctg
     2401 acttcgaaca cgcgttcggg aacaattttt ccggcaaggc cattgtcttg tacaacctgg
     2461 aagagaccat tacggcccag tcgcagctga acgttgagaa cggcgcggat gctgtttatc
     2521 ggcgttccga tagtaatctc agaacgacag aggcgatttc ccgctgggaa ctcaactggc
     2581 tgggatggcc cgggcatgcg ttgcgtttta atgtcgaagc ggccagaaac gtgattggca
     2641 gcgcgctggt gcaggaagtc gactcgggat tcgggctaca ggaagtttcg gttcccggaa
     2701 gcaacacgcg cgttcaggaa gttcgtggtg aactgctgat cggcgacacg tggcgtctgg
     2761 aagagtacgt actcagctac ggggtggggg cggaagcctc gaccatttcg cagagtggag
     2821 attccgtcat gaccagggat ttcctgtttt tcaaacccga actgcaacta acgtattcgc
     2881 agtcgcagga ttggcagaca agacttcgga ttgcacggga agtggcgcaa ctcaatctta
     2941 atgatttcgt cagcgccccg gtttttcagg atgatgatct ggagctcggt aatccagagt
     3001 tggaacccga atcccgatgg gttgccgagc tgagcggaga acgacgtttc ggaagcctcg
     3061 gcgtgatcag agggacactt ttttacaact ggctttcgga agtgcaagac ctggtgccct
     3121 tgacgccggt ttttgaagcg ccgggaaaca taggcgatgg ccgacgatgg ggactcgtag
     3181 tggaagccac acttccgctg tcccagatcg gtatacccgc cgcgaggttg gatatcgaag
     3241 cgcacctcga ggactcagcg gtcactgatc cggttactgg ccatgagcgc gtgctttcgg
     3301 gtgaggcagt cagcggacga ccgatcagtt tcggcgaggg tgagcgatac ggatacgcgc
     3361 tcgagttccg acaggatttc agcgacgcac aaatagcctg gggtttggac atgcgaagcc
     3421 gtgcggaacg aacgagtttc aaagttaacg agcttgctga gtattcggag ggagcggagg
     3481 tcaatgcttt catccagacc acgcgcttgt tcggccagcg gatacggctg tctgcggaaa
     3541 atcttctcaa catgcaggtg ttacgcaccc gcacattgtt tattgggttg cgggcgctct
     3601 ctccggtcaa cgtcatcgag caacgggact tccagaatca gcgacgcata atcttgtcga
     3661 tatccggatc attctgagtt gcgggcggcg tatcgcagct cggcgatcac cagatgtcat
     3721 ctctcacatc agtgccggag acggcttgcc ggccgccggt gtcccggctc ggggctcacc
     3781 tcaatggacg ccaaagtcga cggtcgtccg ggtaccccgc atatcggcaa gccggtcgag
     3841 gtcgaggcgc tgtggctgaa ctcgctcatg atcgggagtg ccttctccgg caagtggcgc
     3901 cagttgtacg cgcgcgcgac ggcctcgttc cgggcgcgct cctggagcga cgagcgcggc
     3961 tgcctcttcg atgtgatcga cgtcgaggag gtttggctgc ggctgcacgg tggaagcgcc
     4021 gaagcgaggc ggacacaaga tttctggcgg ccctgcgcga gcacctggcg atggcagcac
     4081 tcggccatat ctcggaagtt gccgatgccg agccgcccta cgcagcgggc ggatgtcctt
     4141 tccaggcgtg gtctccgggg gagttcttgc gggccagccg tctcgttgcc gcgcggaccg
     4201 gcgccggttg cagttaggat agcggcatgc ctggaagtct caatacgccg gtcggagccg
     4261 agcgttcgcg cctcgcggag gacagccggg gagcggtccg ttggaaactc tgggggccgt
     4321 atctcagcga acgccagtgg ggcaccgtgc gcgaagatta cagcccgcac ggtaccgcat
     4381 gggactactt cccgcacgac catgcgcggt cgcgcgccta tcgctggggc gaggacggca
     4441 tcgcgggcct gagcgatgag cagcagttgc tttgcttcgc gctcgccctg tggaacggca
     4501 aggacccgat cctaaaggag cggctgttcg ggctgaccaa tgccgagggt aaccacggcg
     4561 aggacgtcaa ggagctctac tactacctgg atgccacacc gactcattcc tatctgaaat
     4621 acctctacaa atacccccag gcggcgtttc cctatgccag gctggtcgaa gagaaccgcc
     4681 ggcgcggtcg gaacgacccc gagttcgagc tgatcgatac cggcgtcttc gaagccgatg
     4741 aatacttcga cgttttcgtc gaatacgcga aggccgggcc cgaagacgtt ctcgtgcgga
     4801 tcaccgccca caatcgcggg ccccaagcgg cagcgctgca ccttctcccg caactctggt
     4861 tccgtaacac ctggtcctgg agcgcgaacg cccctcggcc ggagatgcac atcgccgcgg
     4921 acggctcggt cgccgcgctg catgccgagc tcggtgagta ccgcctgtat gccgacggcc
     4981 ggcctgacgt gctgttcacc gacaacgaca gcaaccgcga gcggctgttc ggtgtaagcg
     5041 gtgaccgcgg ccactggaag gatgcgtttc acgaatacgt ggttcacggt cgggacgccg
     5101 cagtaaacgt cgaatgccgg ggcaccaagg cggccctgca ccatcgattg cggatcgccg
     5161 ccggcgaaac cgcgcaaatt cgcctgcgcc tccgccgagg acccgccgcg gtgccgttcg
     5221 aggagttcga gctgtcgttc gggcaacgcg tggccgaagc cgatgggtac tacgcagcgc
     5281 tgcaatcggg acttccttcg gaggacgcca gaagcatcca gcgccaggcg ttcgccggcc
     5341 tgatttggag caagcagttc tatttttacg acattgcgca atggctggac ggggatccgg
     5401 cagagccgcc gcctccgcag agccgccacc acggccgtaa cgccgactgg cggcatctca
     5461 acaaccgcga cattctttcg ctgccggaca aatgggaata tccctggtat gcggcgtggg
     5521 atcttgcgtt ccactgcatt ccgctggccc tgctcgacgc cgcgtttgcg aaacggcaac
     5581 tcgtgacgct ggcgcgtgcc tggtacatgc accccaacgg ccagcttcct gcctacgagt
     5641 gggacttctc gcacgtcaac ccgccggtgc atgcatgggc tgcctggcag gtctttcaga
     5701 tcgatcgcaa gaatcggagc gaccagggcg acctcgcatt cctcgagcgc atcctgcaca
     5761 agctcatgct caatttcacc tggtgggtca atcgcaagga tgcgcagggg cgcaacatct
     5821 tccagggcgg tttcctcggg ctcgacaaca tcggggtgtt cgatcgcagc gcgccgttgc
     5881 cgaccggcgg ccacatcaac caggccgacg gcacggcctg gatggcaatg tactgcctga
     5941 acctcatgcg tatcgccctc gagcttgctc ggcacaaccg cgtctacgag gacatcgcga
     6001 ccaagttctt cgaacatttc ctttatatcg ccgaggcgat gaccaatatg gccggcgcag
     6061 gcatcgggct ctggaacgcg gaggaccagt tcttctacga cgagctgtca ctgccggacg
     6121 gccggctcgt gcccctgaaa gtgcgttcca ttgtggggct gattcccctg tttgcggtgg
     6181 aaacgctcga acccgaattg ctggatgagc tgccggaatt ccggcgtcgt ctcgaatggt
     6241 tcctgaacta tcggccggat ctcacacaac tcgtgtcgcg catggagaag cccggtgtgg
     6301 gtgagcgccg gctcttgtcc ctgttgcgtg ccgatcgcgt gcagcaaatc ctgaggcgcg
     6361 tactcgatca agccgagttt ctttcggatt tcggcatacg ggcgctgtcc aagtaccacg
     6421 agcagcatcc tttcgtgttc gagcacggcg gtgagcgact gtccgtcagc tatcaaccgg
     6481 gtgaatccgg aagcggtgtg ttcggcggca actccaactg gcgcggaccg gtctggtttc
     6541 cggtgaatta tctcctgatc gagtcactgc aaaaatttca ccactattac ggtgacgagg
     6601 tcgggatcga gtgcccgacc ggttcgggct caaggcttac gctcgaagag gtcgccgaag
     6661 aactgacatt gcgcctgagc aggatctttc tgcgtgatgc ggccggacgg cgtgctgtct
     6721 tcggcgagaa ttcgaagctg cagaacgacc cggatttcag ggattacgtg ccgttttatg
     6781 aatatttcca cggagacaac gggcgcggcg ccggcgcttc ccatcagacc ggctggaccg
     6841 gattgatcgc aaggctcctg cagccgtcgg cgtacgaacg gtagtgctga tcgcacggtc
     6901 acagagctgc ggctgatacc tattcagcgt tcgctaacgt tggcagataa gtggtggaca
     6961 tacgcctgag tgtctccggt aatctgcact gagccgcacc agggaggaat gcaatgaaag
     7021 cgacccaagg aggcgcgacg ttttcgccct ttgcacgcac cctggccata gtaatctgca
     7081 tagccgggtc aagcccaacc cgcccaatgg tcaggtgcta gccaaatccg cagtgacggt
     7141 gttagcgagc tggcttcgcc gccgcgctgt tgacctgcct gtcggcccag acgagcactt
     7201 gcaccagcgt gcgtagcagt cgaatggccg gccgtaagat aacgcgggca agcacagaga
     7261 caatccagag gaccggaagc aggatcagca tccagcccag agcctgcgcc ggccacaccg
     7321 gccagcccag cacgtccgcg ataccggcga ggacatcgct gatgatgccc ggacggctga
     7381 ccaccaggta cacgccggca acggcagcgg gccacctggc gtagcgtccg atccgcaccg
     7441 ccatctgccc cgccctacca acgcggctgg catatgcagt agtgcgagcg gaaaagcccg
     7501 catacctcgt ggatgcggcg gttgcccgcc ccatgcgcag cagcttcacg gcgctaccta
     7561 cgaccacggc atccaccgcc gcccagccga catcaccggc accgatgtcc tggtcggtcc
     7621 gaagccttgt ttcgagattg cggataccgc ttgcaaaaaa agcgttggcg ccttcgagaa
     7681 cgcgctcggt ttgtatccac ctgacgttgc cctcaccatc cgtggtgaac tggccgagga
     7741 aatcgtggcc ttcatcacga atgaaattta ccgcgtaccg gccgcgctgt tcgcgtgtca
     7801 gtgagcttcc ctgctccgcc gatcctgcct tcgggattgc gccctcacct tcactctcga
     7861 gttcagatcc agtgccgtgc cagaggcgtt tcgcggcatc ctgcgtttcc cggagcttgc
     7921 gggcggaata gtgcatgaac gcgacggtac tgatttcgtt gttcaggaaa taatgaacag
     7981 gcaggagcac accttcgccg tagttgccca agatctcctg gaacccggct tcggagccat
     8041 agagcggcag aacctcgcga gcgagttccg ggtactttag cagcgccacc ttggctttga
     8101 gcagcagtat cggatcgtcc gcatagtcca gaagaaccgc ttgaacatcg atgggctccc
     8161 ccttcaggac gccccaatac tccggaagcg cttcctctac ctgtatccgg atcaggcgct
     8221 cttccaccga tatcggctgc tgaccggcgg cgatcgccag cgcaccgaca aaagcgagtc
     8281 cgatcagcag gccgagcttt ctcacccggt tctcctctgc atacgtcagg ccacgcgccc
     8341 gacctccgct tcaattcgct ttccgagctt acgctccgcc tcgcgcaagt cataccgagt
     8401 ctgcagattc atccaaaact gcgcgctgtt gccaaagtaa cggctcaggc gcagcgcagt
     8461 ctcaggcgtg attgcccgct tgccattgag tatcgacgta attcggcccg acacttgatg
     8521 atcgccggca gcttcagctc gatggtttcc agcgtggaaa accgttcgcc gcaggcctcg
     8581 cactcgcgcc gtcgtcggat cgtcgcagtg tcgtcgctga cgcgcgagcc gatgacgcgc
     8641 gtgtcatggt tccgtcagaa ggagcagtgc atcctggggg aagccggctc ggttgcggtg
     8701 cggcgagcgc gtttgcctta ttatcacacg cgaccgcgta atgcgccgcc tcgaagatga
     8761 cgccatgcca gccccgctcg aagcatgttc ccccacgcca cgcaccggta ccccgcgccg
     8821 ctggtggccg gcgctgctgc tggtctgcct cttgcccgcg ccgctgctgg cgcaggacga
     8881 cgatgccgcg caggccggcg tcggcttcgc gcagatcgag gtgaccgacc cgatccgcgg
     8941 cggccagatg cccggctatg tgttctatcc ctccccggca cgcgccggat ccccgacgcg
     9001 ggtgggaccg tacgaagtcg agggggtacg cgacgcaccg gcacggcctg gcaccaggcc
     9061 gatggtgctg ctctcccacg gccacgccgg ctccaacctc ggccaccacg acctggcgac
     9121 ctaccttgcc ggccacggtt tcgtcgtcgc ggccatcgcc cacagcggcg acgactaccg
     9181 cgacgccagc ttcgacggcc atccggaagt gctgggcggc cgcccggtcc aggtttcggc
     9241 gacgatcgac gccctgctcc gggacccacg ctggaaaccg ctgatcgatg ccgaccgcat
     9301 cggcgtcgcc ggcttctcca acggcggcta caccagcctg ctgctggtgg gcgccgtgcc
     9361 cagcctgcgg cggttcgtcg actactgccg acgctatccg ggcgaccggc tctgcgcagg
     9421 cgcgaaagac gtcgaggccg aagccgaacg gcaggggcag acgcccgagc agtacatgga
     9481 cgcgatgcag gcccggctga cccactgggg caagacgtcg gatccgcggg tgaaggccgc
     9541 attcgcgatg gcaccgctga gcctggtctt cgacgccgac ggactggccg gcatcgaccg
     9601 gccggtgttc ctctactaca gcgagcgcga caccgtgctg cgaccgtcgg agaacgcggc
     9661 cagaatcagg ccgctgctga agaccctggc cggcgtgcgc accgtgccca gggcggacca
     9721 ttgggtattc attccgccct gcacgcgcga actggccgag gtcgtcgccg agatctgctc
     9781 cgacccgccg ggcgtggacc gcgctcggac gcacgcacgg atcaacgccg acgccctggc
     9841 cttcttccgc aagacgctgg caccgacgac cgacgctccc gccgcgacga aatccggtca
     9901 gccgtagacc gggaactccg cgccctggtc acgttctggt gccggagaga ggactcgaac
     9961 ctccgaccca cgcattacga aagcgacaaa ttgatcgtaa gctttgattc gataaaccat
    10021 tgaccgcggg gcgctcgttg caattgcagt accgtgcact acggcgcatg actcaatccc
    10081 gcagaaatcc cgcaaggatc ttagaatcca aattactggt tgggcgcacg tcgaattgtc
    10141 cggatcggtt ttttgcctgc atcgaacacc tggttcgtag ccacgaacgt taacggccgg
    10201 gtcgccgcgg gcgattgctc acccgcggct cccacagatc cggacgtgcg agattcccgc
    10261 atccggctcc tcagactacg aagtcgctgc gccacggtaa atactgtgaa caacgcgcgc
    10321 ggggggaagt gggtagcggc gacgcaagcg atcgaactgc gcccagttca ggcgcgcctt
    10381 gcgcgaacgg cggctgagcc acttgcgcca gcaacgttcg acctcgtaac ggaagcggag
    10441 aagcgccgtg gcgttccccg tgatcccgta gtaggcgtag tgcccctgga gtttgcgact
    10501 caatgccatc tgttgctcgg ccaccggcaa atgtcgatgc gtccgacacc atgcggtcac
    10561 ccgcttcagc gcacggctga agcgagcctt ggccgtcttg cgcctcacca cccaacgtcc
    10621 ctgccgggaa cgtccccaaa agtgggtgaa ccccaaaagc tcaaagctac gttcgcgttg
    10681 cgaaccctcg cctgagcccg gcggacggca aaagtccacc aaacgggtct tctcgggatg
    10741 cagcgtcaga ccgtacttcc caaatcgttt cggtaatacg gccaacaccc gtctcgcgtc
    10801 ctcctcccgc tcgaacgcca gtactgcatc gtcggcaaaa cgaatttcca tcgctcgccc
    10861 gcgcagtcgc ggtttgactt cttgctcaaa ccacatgtcc agcacttcat gcagatagag
    10921 gttcgacaac agcgggctga tcactccacc ttgaggggta ccccgctccg ggtgcctgac
    10981 caccccttct tccatgaccc cagcattcaa ccacttccca atcgcacggc ggatcacacc
    11041 gtcgcgcacc cgttggccca taaagtgccg aaggtgagtt cggtcaacgt catcgaagaa
    11101 gctctgaata tcgaggtcaa tgatccatcc gcccccgatc tccatcagcc cctgccacag
    11161 cgcctgcagc gcttggtggg cactgcgtcc cgggcgaaac ccatacgagc atgacagaaa
    11221 atcctgctca tacacgggtt ccagcaccat cagcaccgcc cgctgcagaa ccttgtcttc
    11281 caacgtcgga atgccaatgg gccgggtctt cttccgcgta ccgtccttcg gaatgtgaac
    11341 ccgccgcacc gccggcgccc gatagcgtcc ggacttgaac cgcccgagca agctcgacag
    11401 gttcgcctcc agatgcgcct cgtacgcctc ggccgtcaca ccatccactc cgaccgcgcc
    11461 gtccttgcgc gtacgccggt atgcctcgcg caaccacacc tcgtcgatgt gatgcgctaa
    11521 cgtcgaaagc tccaacttcg gctcgatacg agccagtgtc gctagcttct cttgtcgcgt
    11581 tgagatgatt tcgcggctcg atgtcctcgt catctgtccc tcgagaagcc ccatagcccg
    11641 gtctaccgct tccctccatc ggctcccctg gggcaggttc gccgacttca acggtcttat
    11701 cagtagactc cgactcctcg tcctccctcc cgcgtcgctt cgttgccttc gcttcgcggt
    11761 accactgcgt tgcccctctt cgctcccccg ggtcggaacc cctcccgacg gcctggacca
    11821 tttctagcgc ggcgccctcg ccgccttctt cacaatggag aaaacgaggt ctcccaggtt
    11881 cctggacgac ccttgtctgc atgccccgct cttcgacccc ggcggactgc ttgcatcagg
    11941 ccattgcaac gcaagcaatg ctgccttccg cctacttaac gacgtcggct ccgcagctat
    12001 cactatcgag gctctatcac gcggcctgca ggctccctgt gtacgcttcg cagccggggt
    12061 cacccccggt ccacgcaaca ctcggttccg gctggtggcc aacctttgcc ggctcaggac
    12121 ttgcacctgt tgggtcgcaa agaaagtttc cgtcatatgc atccgtctat atgacttcct
    12181 ccttcaccaa gctttgcctg gcgcaataa
//
LOCUS       DASVDU010000182        16994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1375819, whole genome shotgun sequence.
ACCESSION   DASVDU010000182 DASVDU010000000
VERSION     DASVDU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16994
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375819"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..342)
                     /locus_tag="VF226_11885"
                     /pseudo
     CDS             complement(211..342)
                     /locus_tag="VF226_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(531..1490)
                     /gene="hemC"
                     /locus_tag="VF226_11890"
     CDS             complement(531..1490)
                     /gene="hemC"
                     /locus_tag="VF226_11890"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX7074734.1"
                     /translation="MGSPLLRIGTRGSPMALAQTHRVRDALRIANPDLRQNDAIDIVV
                     IQTTADRAQDRTLAEIGGKGLFTKEIEEALLAGSIDMAVHSMKDVPTWLPQGLEIACL
                     LERDDPRDAFISTRATSLAELPAGSVVGTASLRRQAQILHKWPHLRVVPFRGNANTRL
                     RKLEAGTVDATLLALSGLERIGRTDAIAAVLPVEEMLPAVAQGALGIEIRSANDLARA
                     LLAPLSHRSTEVCIAAERSLLATLDGSCRTPIAGLGTLSADKTLTLRGLIASSDGRTL
                     WESCRSGPPSDATRLGADLGQELRRRAGPGLFVGEPGYPRSSL"
     gene            complement(1781..2302)
                     /locus_tag="VF226_11895"
     CDS             complement(1781..2302)
                     /locus_tag="VF226_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7074735.1"
                     /translation="MAVRGGHRPAQQEDGEARWVEHKGRPAVHGFKAHVGADAGTALV
                     EEVAVTLANVNDGRAGPGALPDEPGDVFADSAYRGSHFGNAVRAKGGTPRIVATGMWG
                     HDEQETLARLGVWKQPIHHLRARIEKIFGTWKRSYGLHRMRWRGLAKAAVQVRLTAIA
                     YNLKRGLRIVTAA"
     gene            complement(2927..3814)
                     /locus_tag="VF226_11900"
     CDS             complement(2927..3814)
                     /locus_tag="VF226_11900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7074736.1"
                     /translation="MTAKLPSLKTLQAFDAAARHLSFTKAAEELGVTQGAVSAQIAKL
                     EAELRTELFVRTTRQVRLSDKGAALARACRRAFDGIGREIEIITERPAGNVLTIAVST
                     YVATRWLSRRLIDFLERHPDTTVRFQHSVNADDFEISDVDLAIRWGDGKWQGCKVELW
                     LAMTKRPMCSPRLLRGAHALRSPGDLRHHCLLRDVPTVDLWDKWLRRAGLSRADVPQS
                     TVLSDPVVRVQAAIDGQGVVLADDLAKDEITTGRLVEPFDIELDGYGYFLLRPTDSED
                     RAVVKHFRRWLLRQAARET"
     gene            3976..4959
                     /locus_tag="VF226_11905"
     CDS             3976..4959
                     /locus_tag="VF226_11905"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7074737.1"
                     /translation="MVAKMKSTPEMAFGRDLVIDKKQLKPFVRRTNWHGLVNFSVHMS
                     LLGLAGGLVAVTLGSWWVLPSMIAYSLVMAFLFNPVHECSHGTVFRSRALNETVYWIV
                     CLIYMVPPTFFRYAHATHHTFTQIRGRDPDMMPTQMTLSKYAKFLVGVTFWKRNLLWF
                     LWHPFGYIDPAQRYYLPESEIPRVVREARIIMAIYGVIAALSVYYGSWAAVIFWVLPR
                     LIGEPSMRAVRIAEHAECHEGGDLRKNTRTTRASAILRFLFWNMPYHAEHHLCPMVPF
                     HALGRLHGVVGHKLHPVGDSYPAVHAEVIDKIRRHEGVTWAPGIAHAEQTR"
     gene            4976..5293
                     /locus_tag="VF226_11910"
     CDS             4976..5293
                     /locus_tag="VF226_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018209622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX7074738.1"
                     /translation="MSGNWHEVAVVGDIDEDDVIQVEIAGRVLAIYNSGGEFFASDGI
                     CTHEFACLADGLVMDGIIECPLHQGRFDIKTGKAKSPPVSVDLKTYPVRVEGTAIHVQ
                     LDD"
     gene            5302..6519
                     /locus_tag="VF226_11915"
     CDS             5302..6519
                     /locus_tag="VF226_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074739.1"
                     /translation="MAPSQAHVIVGGGQAGACAAMAMREAGFDGPITVIAEEPHPPYE
                     RPSLSKELLRTGAGPVFIWPSGWQDENGVTLLSGTRAMHLDLDRHVVTTSDYGEIAFN
                     RLLLATGGIVRRLELAGTHLAGICALRSLDEASALRVALTTARRLIVIGGGLIGLEVA
                     ASARRLGVDVTVLEAAPALLGRIAPAEIAALALQTHLEHGVDIRLNVQPASFLGRTHL
                     QSVKLVDGTVIEGDLAVVGIGITPATDLAARAGLAVDDGILVNQRCRSSHRRVYAAGD
                     CARQMRPNGGRALRLENWTNARKQAAVAARNMCGANEAFDEVPWGWSEQYDLLLQFAG
                     APLCWTNRIVRRLRNGLLVLELDGGIVTAAAGVNCSKEMLLASHMIKARTHVDPTLLQ
                     DPSQPLKAMLKSA"
     gene            6535..7317
                     /locus_tag="VF226_11920"
     CDS             6535..7317
                     /locus_tag="VF226_11920"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7074740.1"
                     /translation="MAKLSVRELLDGKGTCQRTFVQVTTAEQAVAAEEAGIEMIGTAY
                     LEERRRIPNAVPNTHFQFGLQYGKYVNADEAMRAAFSAIEDGATTIYCAMSTGVIERL
                     AREGVPVIGHVGLVPPLATWVGGLRAVGKSPEQAVAIYRKVKDLEAAGAFAVEMEIIP
                     HRLASEISRRTSLFTISLGSGRGCDAQYLFSADILGEASRVPRHAKTYRNLAAEYTRL
                     QGERVAAYAEFAADVGAGRFPSSAHTVEMTESDFERFLHEIE"
     gene            complement(7415..8722)
                     /gene="hisD"
                     /locus_tag="VF226_11925"
     CDS             complement(7415..8722)
                     /gene="hisD"
                     /locus_tag="VF226_11925"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX7074741.1"
                     /translation="MSVTYLKKAARTAETDQGDVRATVQVMLDELEAGGDDVARRFAR
                     DLDKWEGDILVAPEVVEAAREFVPQRLQDDIRFAHDNIRRFAEAQKETIADCSIEIVP
                     GFWAGQKQIPVSAAGCYVPGGRYSHIASAMMTITTAKVAGVSHIAACSPPRPGLGISP
                     ATLFAMDLCGADQILNLGGVQGVAAMALGLFGLPKADILVGPGNQYVAEAKRILFGKV
                     GIDMIAGPTDSMVLADETADPQVVAWDLIGQAEHGYNSPVWLVTTDPALAEAVMREAP
                     ALIGQLPELNAKNAEAAWRDYAEVILCSDREEMAAVADRYAPEHLQVQATDLGWWLGR
                     LRSYGSLFLGEETTVAFGDKASGPNHVLPTSGAARYTGGLSVHKFMKTVTWQRGTREG
                     AKVIAEATARISRLEGMEGHARTADVRLEKFFPGERFELSVSN"
     gene            complement(8750..9631)
                     /locus_tag="VF226_11930"
     CDS             complement(8750..9631)
                     /locus_tag="VF226_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7074742.1"
                     /translation="MMDHGVRNAARTRRSLPGVPARLSRGRPTKPGETYGVPGAGDTR
                     WLSLATIGVLLFVWWLVTAMGLVKPLFVPSPQAVVEKFFVIWNQGFTGTPLWGHVLIS
                     TGRVFGAFMLACIVGIPLGLAMGVSPMIRGIFDPPIEFYRPIPPLAYLPLMIIWFGIG
                     ETSKVLLIFLSVFAPVALGARAGVRSAAIEQIHAAYSFGATRWQVLRHIILPAAMPEI
                     LTAMRIGIGFGWTTLVAAEMVAATRGIGYMVLSASQFLQTAVVIMGIIVIAVIAYLFD
                     LLMRAIERRVVPWKGKM"
     gene            complement(9628..10413)
                     /locus_tag="VF226_11935"
     CDS             complement(9628..10413)
                     /locus_tag="VF226_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7074743.1"
                     /translation="MGLQIDNACVVYPAVGGRPATEALQQINLAVDDGDFVVALGASG
                     CGKSTLLNLIAGFLAPTSGTIRLDGASIDGPGADRSVVFQKHALLPWLNVRDNVAFGL
                     MLQGHAKADRYKVGDHFIELLGLHGFENAPVYALSGGMQQRVGIARALTCDPKLLLMD
                     EPLGALDALTREKAQELILRMWNDTGKSVFFITHSVEEALFMGTKLIVMSPRPGRITH
                     RFHLPFSRMFLEGCSARSVKASSEFIALREEVLSIIFSDEEAA"
     gene            complement(10485..11483)
                     /gene="tauA"
                     /locus_tag="VF226_11940"
     CDS             complement(10485..11483)
                     /gene="tauA"
                     /locus_tag="VF226_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_function: GO:0030977 - taurine binding [Evidence IEA];
                     GO_process: GO:0015734 - taurine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7074744.1"
                     /translation="MKNKRWGVFFGSLIASAMLATAAQAAEKVIVATFGDPTPAQWAA
                     HEHKFADATGWDVEWRKFASGTDVIAAMASGDIKIAELGSSPLAIAATQGVDLEMFMI
                     EYVIGNSESLIVRNGSGIKTLADLKGKRVAVPIGSTAHFSLMGALKHAGIAEHELTIM
                     NMSPDQIAAAWQQSAIDAAFVWPPTQTEILQTGERLIGAGEVAAWGYPTFNAWVVNRD
                     FAASHEKGLIAFIRTFDDANEAYLKDPAAWTADSAPIKAIAAQTGADPRQVPETIQGY
                     TFLPLAEQLKPQWLGGSIAKTLKATAEFLKSAGRLDDVADDYSSYVTVDYVKKAVR"
     gene            complement(11603..12388)
                     /locus_tag="VF226_11945"
     CDS             complement(11603..12388)
                     /locus_tag="VF226_11945"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX7074745.1"
                     /translation="MTAASPKRRIGILGGMGPEATVLLMSRIVAMTPAQDDSDHVPLL
                     VDNNTQVPSRIKAIIEKTGEDPGPVLVDMARRLANSGVAALAMPCNTAHHYAGLIAAA
                     VPMIPFLNMIELSADHVAELERNLRNRASRRVGMLASPAVRVTGIFDRAFAARRLETL
                     YPSDPNRMLACIKAIKADSRDAKARGILKESAEELRAEGADVLLVACSELSIIADVIP
                     KDLPVVDTIDVLAGAVVAFSGGPSVREISGGPIDDRAAPGGSA"
     gene            complement(12385..13596)
                     /locus_tag="VF226_11950"
     CDS             complement(12385..13596)
                     /locus_tag="VF226_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX7074746.1"
                     /translation="MKLRPFKGNFTQQEPISEEGIAAAVEVLRSGRLHRYNTLVAETS
                     EASMLEREFSAYQGSRYCLACASGGYALQITLRAAGLEPGEPVLTNAFTLAPVPGAIV
                     NAGGRAIFVETTRDLVIDLDDLERKASASKARFLLLSHMRGHMPDMDALLALVVRRGL
                     ILIEDCAHTMGASWDGRKSGSFGLAGCFSTQTYKHMNSGEGGLLTSEDAEFMARAIIM
                     SGSYMLYERHGAAPDPAVFADIRLDTPNFSGRMDNLRASILRPQLAELENNIERWNER
                     YRILQAKLRGANAVYLPVRPLQERYVGSSIQFLIPGISAEAARAFVGACASLGVELKW
                     FGDQEPVAFTSTHKNWRYLQPQALPATDEILSGLFDMRIPLTFTTADCSHIGDIIVAS
                     TAALSPEGAAQ"
     gene            complement(13617..14645)
                     /locus_tag="VF226_11955"
     CDS             complement(13617..14645)
                     /locus_tag="VF226_11955"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HEX7074747.1"
                     /translation="MHLARFPRIRLAHLPTPLEPLERLSRHLGGPEIWIKRDDCTGLS
                     TGGNKTRKLEFLMAEARAQGADLVMTQGATQSNHARQTAAFAAKLGLSCHILLEDRTG
                     SRDPNYNTNGNVFLDFLHGATAEKRPGGMDMNAEMEAVAKGFRAEGRKVYVIPGGGSN
                     PTGALGYVNCALELVSQANDRGLVIDHIVHATGSAGTQAGLVTGLKTINANIPLLGIG
                     VRAPKAKQEENVFKLAAATAEKLGCPGVVAREDAVANCDYVGEGYGIPAASTIEAIEM
                     FAQIEGILLDPVYSGKGAAGLIDLIRKGHFKKGERVVFVHTGGSAALFGYTAAFDFGQ
                     RAAMAAAQ"
     gene            14761..15741
                     /locus_tag="VF226_11960"
     CDS             14761..15741
                     /locus_tag="VF226_11960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7074748.1"
                     /translation="MRLEWIEDILAILRAGSLSRAAEQRYLTQPAFSRRIRSIEEYLG
                     VELVDRTRKPAQLRRSVLEKQQRLEELAEGLHNLLYELREQDRKIENRVTIASQHAIT
                     TSVAPTLVKRLSTDTDTHIRLRSANRDECFNLLVTRQADLTLTYRSPDEQLPLEGAYL
                     EKCDLGEEQLIPVFATDALSALNDGYRAGELPVIAYPADVFLGEVMNREIFPKLRSQS
                     FLRAKAETALTLAALQLALTGVGVAWVPRSLAAKDIAYGNLTELSHIFSKARLTIGAI
                     RLAGSKSPAEQEVWNIIGGHTASSGEQTSAAIGEARADRCRITSAPAPSE"
     gene            complement(16451..>16994)
                     /locus_tag="VF226_11965"
     CDS             complement(16451..>16994)
                     /locus_tag="VF226_11965"
                     /inference="COORDINATES: protein motif:HMM:NF036996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074749.1"
                     /translation="GPLGWIIEGHLHLLVRLEPYRVPLIRPHPGVTAAVYTLGADIGG
                     DGRLVAAAMDVGSDGLVVLGMGAGHVAPGMVAALEAAATEIPVVLARSVADGPILSSS
                     YGYAGAETDLLSRGLISAGWLSSRKAHALLSLLVMGRAQRKEIGDAFRCLGAPRIGRP
                     PAIRRSLGSFARHHLGCEPD"
BASE COUNT         3282 a         5356 c         5244 g         3112 t
ORIGIN      
        1 ttgctgccct tgccaaccag gagcatggtt gtcttcgaag tcgttctggc gggtccacat
       61 gattatcgat ccgccacctc atgatcgcgt tcacaggcgt cggctgccct gcgtctgctc
      121 cacattcaga tggcgcagat actacctcgg actcagccct tgcctctcgt cctggcggac
      181 gagaactctg caaggcgatt caactcattc ggcatcgcgt ccctccttct cgccgcactt
      241 cgtgatccgg cccgtatagt ccatctgacc cgactgatat cgtcgcgctg tcaagctcgc
      301 atgggcgcgg accgtgcgcg atttagtgaa ccggctcggg tcgctttgag ttcgaaagta
      361 tgtgctgccg cacgagagaa tcctcaccac ccctgagaat cgcgacgcgg aagcggcgat
      421 cgcgactttc aagtagctat ccgcctactt gaccaaagtg tacgccacca tgcgcgaatg
      481 gaagcgccga cgccaatcaa cgaagagctc gcccgtggct taggcatcgg tcaaaggctt
      541 gatcggggat agccgggctc acccacgaag aggccgggac cggctctcct ccgcaactcc
      601 tgcccaaggt ccgcgcccaa tcgggtagca tccgatgggg gaccagagcg acaactctcc
      661 cacagggtcc gtccgtcaga gctggcgatc agacctcgga gggtcagagt tttgtcggca
      721 gacagagtgc cgagaccggc gatcggcgtc cggcaggagc cgtcgagcgt ggccaaaagg
      781 ctgcgctcgg cggcgataca gacttcggtt gaccggtggc tcagcggcgc aagcaacgcg
      841 cgggcgagat cgttcgcgct gcggatttcg atgccgaggg cgccttgcgc aacagcgggc
      901 agcatctcct cgacaggcag aactgcggcg atcgcgtccg tccgcccgat ccgctcaaga
      961 ccagacaacg cgaggagcgt cgcgtccaca gtaccagcct ccagctttct taggcgcgtg
     1021 ttcgcgttgc cacggaacgg cacgacccta agatgcggcc acttgtgcaa gatctgcgcc
     1081 tgccggcgca gcgatgccgt tccgacaacc gatccggccg gaagctccgc aaggcttgtc
     1141 gcacgtgttg aaatgaacgc atcgcgcgga tcgtcgcgtt ccagcaagca agcgatttcg
     1201 agaccttgcg ggagccaggt cggaacatcc ttcatggagt gaacggccat atcgatcgat
     1261 ccagcgagca gagcctcctc gatttctttg gtgaagagcc ccttccctcc gatctctgcg
     1321 agagtcctat cctgggcgcg atcggccgtc gtctgaatga ccacaatatc gatggcatcg
     1381 ttttgccgga gatctggatt cgcaatgcgt aacgcatccc taacgcgatg cgtctgcgcg
     1441 agggccatag gactcccgcg ggtgccaatg cgcagtagag gtgaccccat gcttcctcca
     1501 atctttcgga ccggcttata ggaggaacgg agcatccgag caacgttgaa cgtcaattgc
     1561 tcggattctc gagaccatca catcaagcga acgcagaaac tacttcgcgc aagcaggata
     1621 tgaccaaacc caaagtcatc ccgctctagt ggagacctgt gcgcgggtcg cgctggacgc
     1681 atgattatcc gctattcggc cgcgtaacgc gtgttggcgg tgtcggatgg ggggaccggg
     1741 ctcgccaagg tgcgcggcgc tcatggtgtt gcggtgctcg tcaagccgcc gtaacgatcc
     1801 tcagaccacg cttgaggttg taggcgatgg ctgtgagacg aacctggaca gcggccttgg
     1861 ccagtcctcg ccaccgcatg cgatgcaggc catagcttcg cttccaggtg ccgaagatct
     1921 tctcgatgcg ggcccgcaga tggtggatgg gctgtttcca gacgccgagc cgcgccaaag
     1981 tttcctgttc gtcgtggccc cacataccgg tggcgacgat gcgcggcgtg ccacccttgg
     2041 cgcggacggc attgccgaaa tggctgcccc gataagcact atcggcaaag acatcgccag
     2101 gctcgtcagg caaggcaccg ggaccagccc tgccgtcatt gacattggcg agcgtgacgg
     2161 cgacttcttc gaccagagcc gtgccggcat cggcgccgac atgggccttg aagccatgga
     2221 cggctggcct gcccttgtgc tcgacccagc gcgcttcgcc atcctcttgt tgagctggcc
     2281 ggtgaccgcc tcgaacagcg atttgcccag gtcatgagcg acaagggcct tgcggaagcg
     2341 aacgaaggcc gtgcgttccg gcgttggctc cgacgatgaa aacccgcaaa accgcaccaa
     2401 tccgtcgacg cgccatcgat cacctcaatc cttgcacaaa ctgagggaat caaaagcaac
     2461 gcgccacgtc cagcgccact cgcgcacagg tctcctaaaa gagggtggcg taccaatagg
     2521 gttacgaagc tgagaattgg cgccttacgg tgccgtatcg caatttattt tagatcgcgt
     2581 cacatcttcg accatctcgc gctagaaggg aacgtcagcg cgaccaatcg cgcgcgagcg
     2641 cggcgtggcc ttgagcttgc ttgccaatcg gctggctgct cacgcgatcc ggcgccccgg
     2701 ccgttcccgc tcttgcgcgc ggcagcatcg agacaatgcc ttcatcagcg gtcgcgcggc
     2761 catcagctat ggcgagcgga ccggcgccaa ccccggcgcg gtgatccgtg ggtgccagtg
     2821 agcggcaggg cgcggggcat gacgcgcgac gaggaaacga gccgcgcctg acgcgacggg
     2881 ggactgatcc cggcacgccg agacctggat cgccgtcgtc gccgggtcag gtctcgcgcg
     2941 ccgcctgccg caggagccac cgccggaaat gcttgaccac ggcacgatcc tccgaatccg
     3001 tgggccgcaa aagaaagtag ccatagccgt cgagctcaat gtcgaacggc tcgaccagcc
     3061 ggccggtcgt gatctcgtct ttggccagat cgtcggccag aaccacgccc tggccgtcga
     3121 tcgcggcttg aacccggacc acggggtccg acaacacggt cgactgcggg acatcggcgc
     3181 ggctgagccc ggcccgcctg agccacttat cccagaggtc aaccgtcggc acgtcgcgca
     3241 acaaacagtg atgtctgagg tcgcccggcg atcgcaaggc gtgcgccccc cgcaggaggc
     3301 gtgggctgca catcggacgt ttcgtcatgg caagccacag ttcgaccttg cagccttgcc
     3361 attttccatc gccccagcgg atggcgagat cgacatcgct gatctcgaaa tcgtcggcat
     3421 tgaccgaatg ctggaagcgc actgtggtgt cgggatgccg ctccagaaag tcgatcaggc
     3481 ggcgggacag ccaccttgtg gcgacatagg tgctgacggc gatggtaaga acattacccg
     3541 ccggccgctc ggtgatgatc tcgatctctc ggccgatacc gtcgaaggcc cggcggcacg
     3601 cccgggcaag cgctgcgccc ttgtcgctca agcgaacctg tcgggtcgta cgcacgaaga
     3661 gttcggtgcg tagctctgcc tcgagcttcg cgatctgcgc actgacggcg ccttgggtga
     3721 caccaagttc ttcagctgcc ttggtaaagc tcaagtgccg cgccgcggca tcaaaggcct
     3781 gcagggtctt taatgagggc agcttggcgg tcatgctgta ccccaattca tttcctcagc
     3841 taatgaatat agagaaaaat tcgtttgtcg gcacaacacg ttatgtgcag gcatagagtc
     3901 taacaaatta tctcacctat cggatgtagg ctccaggact atccgtggag accgacactt
     3961 ccggaggttg gaggcatggt cgcgaagatg aaatccacac ccgaaatggc ctttggccgg
     4021 gatctggtta tcgacaagaa gcagttgaag ccgttcgtta ggcgcacgaa ctggcatggg
     4081 ctcgtgaact tctcagtcca catgagcctt ctgggtctgg cgggcgggtt ggtcgcggta
     4141 acactcggca gctggtgggt cctgccgagc atgatcgcgt attccctagt gatggcgttc
     4201 ctcttcaatc ccgtccatga atgcagccac gggacggtat ttcgcagccg ggccctgaac
     4261 gagaccgtct actggatcgt ctgtctcatc tacatggtgc cgccgacgtt cttccgctac
     4321 gcccacgcca cgcaccacac ctttacccag attcgggggc gggacccgga tatgatgcct
     4381 acccagatga cgctgtcgaa gtacgcgaaa ttcctcgtcg gggtgacgtt ctggaagcgc
     4441 aatctcttgt ggttcttatg gcatcccttc ggctacatcg acccggcgca gcgttactac
     4501 ctgcccgaaa gcgaaatccc gcgggtggtg cgcgaagcgc ggatcatcat ggccatttac
     4561 ggggtgatcg cggcactctc ggtctactac ggctcgtggg cggcggtgat cttctgggtg
     4621 ctgccgcgcc tgatcggtga gccttctatg cgcgcggtgc ggatcgccga gcacgccgaa
     4681 tgccacgaag gcggcgatct gcgcaagaac acgcgcacga cgcgggcttc tgcgatcctg
     4741 cgcttcctgt tctggaacat gccctatcac gctgagcatc acctatgtcc catggtgcca
     4801 ttccatgcgc tcgggcggct gcacggagta gtgggccaca agcttcatcc ggtgggcgac
     4861 agctatcccg cagtgcatgc cgaagtgatc gacaagatca ggcgccacga gggcgtgacc
     4921 tgggcgcctg gcattgcgca cgccgagcag acaaggtgag gaggtgccga gcgacatgag
     4981 cgggaattgg catgaagtgg cagtcgtcgg agacatcgac gaagacgacg tgatccaggt
     5041 cgagatcgcc ggtcgggtgc tggcgatcta caactccggc ggcgagttct tcgcttccga
     5101 cgggatctgc acccacgagt tcgcctgtct ggccgatggg ctggtgatgg acggcatcat
     5161 cgaatgcccg ctgcaccagg gccggttcga catcaagacg ggcaaggcca agtccccgcc
     5221 ggtgtcggtc gatctcaaga cctatccggt gagggtcgag ggcacggcca tccatgtcca
     5281 actcgacgac tgaggttttg cgtggcacct tcacaggctc acgtgatcgt cggaggcggg
     5341 caggccggag cctgcgctgc gatggccatg cgcgaagcgg gatttgacgg cccaatcacc
     5401 gtgatcgcag aagagcccca ccctccctat gagcggccgt cgctttcgaa ggaactgctc
     5461 cgcaccggcg ctggacctgt cttcatctgg ccaagcggat ggcaagacga aaacggcgtg
     5521 actctgcttt cgggaacacg tgcgatgcat ctcgacctcg atcgtcacgt ggtcacaacc
     5581 agcgactacg gcgagatcgc ctttaatcgg ctgcttctgg ccacaggagg cattgtccgc
     5641 cgccttgagc ttgcgggtac ccacttggcg ggtatctgcg cgctgcgctc gctcgatgag
     5701 gcgtctgcct tgcgcgttgc gctgacgacc gcacgcagac tgatcgtcat cggtggcggc
     5761 ctgatcggcc tcgaggtcgc cgccagcgcc cgcagacttg gcgtcgatgt caccgtcctg
     5821 gaggctgccc cagccctgct cgggcgcatc gcgccggcgg agatcgccgc tctggcgctg
     5881 cagacgcatc tcgagcacgg cgtcgacatt cggctcaacg tgcagccggc cagtttcctt
     5941 ggtcgcacgc accttcaaag cgtgaagctt gtcgacggca cggtaatcga aggcgacttg
     6001 gcggtcgtcg ggatcggcat aaccccggcc acggacctcg cggcccgcgc cggccttgcc
     6061 gtggacgacg ggatcctcgt caaccagcgc tgccggtcct cacaccggcg cgtttatgct
     6121 gccggcgatt gcgcacggca gatgcggccg aacggcggtc gcgcgctcag gctggagaac
     6181 tggaccaatg cacgcaagca ggcggccgtc gcggcccgca acatgtgcgg cgcgaacgag
     6241 gcgttcgacg aagtgccctg gggctggtcg gagcagtacg atctcttgct ccagttcgcc
     6301 ggtgcaccgc tttgctggac caataggatc gtccgcagac ttcgcaacgg gcttctcgtt
     6361 ctcgagcttg acggcggcat cgtcaccgct gcggccggag tcaattgcag caaggagatg
     6421 ttgctcgcaa gccacatgat caaagcacgc acgcatgtcg atccgaccct gctccaggac
     6481 ccctcgcagc ccctcaaggc gatgctcaaa agtgcgtaaa gcaggacgta cgctatggcc
     6541 aagctgagcg tccgcgagct gcttgatgga aagggcacgt gccagcgtac cttcgtgcaa
     6601 gtcaccacgg ccgagcaggc ggtcgcggcc gaggaagccg gcatcgaaat gatcggcacc
     6661 gcctatctcg aggaacgaag gaggatcccg aacgctgtac ccaacaccca cttccagttc
     6721 ggcctgcaat acgggaagta cgtgaacgca gatgaggcaa tgcgcgcagc cttcagcgcg
     6781 atagaggacg gcgccaccac catctactgt gcaatgagca ccggcgtcat cgaacgtctg
     6841 gcgcgcgaag gcgtgcccgt gatcggccat gtcggcctcg ttccgccgct tgccacctgg
     6901 gttggcgggc tgcgcgcggt gggcaagtcg cctgagcagg ctgtggccat ctatcggaag
     6961 gtcaaagacc tcgaggctgc cggcgcgttc gctgtcgaaa tggagattat cccgcatcgg
     7021 ctggcgagcg agatctccag gcggacatcg ctgttcacta tttccctggg ttcgggccgc
     7081 ggctgtgacg cgcagtacct gttttccgcc gacatcctcg gcgaggcctc gcgcgtgccg
     7141 cgacatgcca agacgtatcg aaaccttgcc gcggaatata cgcgtttgca gggcgagcgc
     7201 gtggcggcct atgcggagtt cgccgctgat gtcggcgctg gcaggtttcc ttcctcggcc
     7261 cacacggtcg agatgaccga gtcggatttc gagcgctttc tgcacgagat cgagtgatca
     7321 aacgccttga tgactccgcg gggaaaacac gcccgtcggg ccggaggcct ccgtcctcat
     7381 gagggttgca cggaagcgga taagcaactc cgacttaatt cgaaacggac agctcgaacc
     7441 tctcgccggg aaagaatttt tctagtctga cgtcggcggt gcgggcgtga ccctccatgc
     7501 cttccaagcg tgaaattctt gccgttgctt cggcaatgac ctttgcgcct tcgcgcgtcc
     7561 cgcgttgcca agtgaccgtc ttcatgaact tgtggactga cagtccgccg gtgtagcgcg
     7621 ccgctcccga ggtcggcaga acgtgattgg ggccggaagc cttgtcgccg aaggccaccg
     7681 tcgtttcctc tccgagaaac agcgacccgt aggacctgag gcgccccagc caccagccga
     7741 gatcggtcgc ctgcacctga aggtgttccg gcgcatagcg gtccgctacg gctgccattt
     7801 cttcgcgatc gctgcaaaga ataacctccg catagtcccg ccatgcggct tcggcgttct
     7861 tggcgttcaa ttccggtaat tgcccgatca gcgccggcgc ttcgcgcatc accgcttcgg
     7921 ccagggccgg atctgtggtc acaagccaga ccggagaatt gtagccatgt tcggcttggc
     7981 caatcagatc ccaagccacc acctgggggt cggcggtttc gtccgcgagc accatcgagt
     8041 ccgtcgggcc cgcgatcatg tcgatgccga ccttcccgaa gaggatgcgc ttggcctcag
     8101 ccacgtactg gttgcccggg ccaacgagga tgtctgcctt gggcaagccg aacaggccca
     8161 gcgccatcgc cgcgacgccc tgcaccccgc cgaggttgag gatctgatcc gctccgcaaa
     8221 gatccatggc aaaaagcgtc gccggcgaaa tgccgaggcc cggccgcggc ggtgaacagg
     8281 cggcgatgtg cgaaacgccg gccaccttgg ccgtcgtgat cgtcatcatg gcggacgcga
     8341 tgtggctgta gcggccgcca ggcacataac aacctgcggc gctcacgggt atttgtttct
     8401 gtccggccca gaagcccggc acgatttcga tggaacaatc ggcgatggtc tctttctgag
     8461 cctctgcgaa acgccggatg ttgtcatggg cgaagcggat gtcatcctgc agccgttgcg
     8521 ggacgaattc cctggccgcc tcgacaacct ccggcgcgac caggatgtcg ccctcccact
     8581 tgtcgagatc gcgtgcgaag cggcgggcga catcgtcccc tccagcttcc agctcgtcga
     8641 gcatgacctg gaccgtcgcc cgcacatcgc cctggtcagt ttctgccgtt cgggcggcct
     8701 tcttcaaata ggtaacactc ataaccgaac tccagccatc taagcggatt tacattttgc
     8761 ccttccaggg cacgaccctg cgttcgatgg cgcgcatgag aaggtcaaag aggtaggcaa
     8821 tcaccgcgat gacgatgatt cccataatca caactgcagt ctgaagaaac tgggaggccg
     8881 agagcaccat gtaaccgatg ccccgtgtcg ctgcgaccat ctcggccgcc accagcgtgg
     8941 tccagccgaa gccgatgcca atccgcatag ccgtcaggat ttccggcatg gccgccggca
     9001 ggatgatatg gcgcaagacc tgccagcgcg tcgccccaaa ggaataggcc gcgtgaatct
     9061 gctcgatcgc cgcggaacgc acgccggcgc gcgcgccgag ggcgacaggt gcgaagaccg
     9121 acaggaagat cagcagcact ttcgaggtct cgccgatccc gaaccagatg atcatgaggg
     9181 gaaggtaggc gagcggcgga atcggtcgat agaactcgat tggcgggtcg aaaatacccc
     9241 ggatcatggg actgacgccc atggccagcc cgagcgggat tccgacgata caggcaagca
     9301 tgaaggcacc gaagacgcgt ccggtcgaaa tcagcacgtg gccccacagc ggcgtgccgg
     9361 tgaacccctg gttccagatg acgaagaatt tctccacgac cgcctgcggt gagggcacga
     9421 acagtggctt caccaatccc atggcggtga ccagccacca gacgaacaac aggacaccga
     9481 ttgtcgcaag acttagccac ctggtgtcgc ccgccccggg aacaccatag gtctccccag
     9541 gcttggtggg ccgcccccgt gaaagtcgtg ccggcacgcc agggagggag cggcgcgttc
     9601 tggcagcatt cctcacgccg tgatccatca tgcggcctcc tcgtcggaga agatgatgct
     9661 cagcacctct tcgcgaagcg cgatgaattc ggacgacgcc ttgaccgagc gggcgctgca
     9721 tccctctaga aacatacggg aaaaaggaag atggaagcga tgggtgatcc gacctggacg
     9781 tggggacatc acgatcagct tggtacccat gaacagcgcc tcttcgacgc tgtgagtaat
     9841 gaagaacacc gatttgccag tatcattcca catacgtagg atcagttcct gcgccttttc
     9901 acgggtcagc gcatcgaggg cgcccaacgg ctcatccatc agaagcagct tgggatcgca
     9961 ggtcaacgca cgggcaatgc cgacacgctg ctgcatgccg ccggacagcg catagacggg
    10021 cgcattttcg aagccgtgga gcccgagcaa ctcaatgaag tggtcgccga ccttatagcg
    10081 atccgccttg gcatgaccct gcagcataag gccgaaggcg acgttgtcgc ggacattgag
    10141 ccaaggcaac agggcatgct tttggaaaac aaccgagcgg tccgccccgg ggccgtcgat
    10201 gctggccccg tcgagccgga tcgtcccgct cgtcggcgcc agaaagccgg cgatcaggtt
    10261 caagagggtg gatttgccgc agcccgaagc cccgagggcg acgacgaagt cgccgtcgtc
    10321 gacggccaag ttgatctgct gtaaggcctc ggtcgcaggc cggccaccaa ccgccggata
    10381 gaccacgcac gcattgtcga tctgaagccc catcttcaag gttactccct gcccgatggc
    10441 cccggcctcc atggggaggc cgggatggat ttgcctgcgg gcaatcagcg aacagccttc
    10501 tttacgtaat caactgtaac atagctcgaa taatcgtctg caacgtcgtc gaggcgacca
    10561 gcggatttga ggaattcggc cgttgctttc aaagttttgg caatcgagcc ccccagccac
    10621 tgcggcttca gttgctctgc caggggcagg aaagtgtagc cctggatggt ttccggcacc
    10681 tgtcgcggat ccgcgccggt ctgggcggcg atcgccttga tcggcgcact gtcggcggtc
    10741 caggcggccg ggtccttgag ataggcctcg ttggcatcgt cgaaagtgcg gatgaaggcg
    10801 atcaggccct tctcgtgcga cgccgcgaag tccctgttca caacccaagc attgaaggtg
    10861 ggataacccc aagcggccac ctcaccagct ccgatgaggc gctcgccggt ctggaggatc
    10921 tcggtttgcg tcggcggcca gacgaacgcc gcatcgatgg ccgactgctg ccaagccgcc
    10981 gcgatctggt ccggcgacat gttcatgatg gtcagttcat gttcggcgat accagcgtgc
    11041 ttcagcgccc ccatcaggga gaagtgggcc gtcgagccga tcggcacggc gacgcgcttg
    11101 cccttgagat cagccagcgt cttgatcccg gaaccgttcc ggacgatcaa cgactccgag
    11161 ttgccgatga cgtactcgat catgaacatc tccagatcga cgccctgcgt ggcagcgatg
    11221 gcgagcggcg aggagccgag ttcggcaatc ttgatgtcgc cagaagccat ggccgcgatc
    11281 acgtccgtgc cagacgcgaa tttgcgccac tccacgtccc atccggttgc atccgcgaac
    11341 ttatgttcat gggccgccca ctgcgcgggc gtcgggtcgc cgaaggtggc cacaatgacc
    11401 ttttccgcgg cttgcgccgc tgtcgccagc atagccgagg cgattaggct gccgaaaaat
    11461 actccccagc gcttgttctt catttgaagc tcctgttccg attggttcat ggtgaaccgg
    11521 caacggcgct acctcgccac gccggtcctt cttggggtct cctcgcagag acggaaaacg
    11581 accctccttg cctgaagcgc cgtcacgcgg atccaccggg cgccgcccgg tcgtcgatcg
    11641 gcccaccaga gatttccctg accgacgggc cgcccgaaaa tgccaccacg gcgccggcca
    11701 gcacatcaat cgtatcgacg acgggaaggt ccttcgggat gacgtcggcg atgatcgaca
    11761 attcgctgca ggcgaccaaa aggacgtcgg ccccttcggc gcgcaattcc tcagccgatt
    11821 ccttgagtat tccgcgtgct ttggcgtcgc ggctgtccgc cttgatggct ttgatgcagg
    11881 ccagcatgcg gttcggatcc gacggataaa gagtctccag gcggcgggcg gcgaaggcgc
    11941 ggtcgaagat tccagtcacc cggactgcgg gagacgccag catgccgaca cggcgtgatg
    12001 cacgattgcg cagatttcgc tccagctccg cgacgtggtc ggccgacagc tcgatcatgt
    12061 tgaggaaggg aatcatcggg actgccgcgg cgatgaggcc agcataatgg tgcgccgtgt
    12121 tgcagggcat cgccagggct gcaacgccgg aattggccaa acggcgcgcc atgtcgacga
    12181 ggactgggcc cgggtcttcg ccggtcttct cgatgatcgc cttgatccgc gacggtacct
    12241 gcgtgttgtt gtccaccagc aagggcacgt ggtcgctgtc gtcctgggct ggcgtcatcg
    12301 cgacgatgcg cgacatcaag aggaccgtcg cctcgggtcc catgccgccg agaatgccga
    12361 tccggcgctt gggcgatgcg gcagtcattg ggccgctccc tccggcgaca gggccgcggt
    12421 cgacgctacg atgatatcgc cgatgtgtga gcagtccgcg gttgtgaagg tcaaagggat
    12481 ccgcatatcg aaaagccccg acaggatctc gtcggtcgct ggcagagcct gcggctgcag
    12541 gtatcgccag ttcttatgag tgctggtgaa agccaccggc tcttgatcgc cgaaccattt
    12601 gagttcgacg cccagagaag cgcaggcgcc gacgaaagca cgcgcggcct ctgccgatat
    12661 gccggggatg agaaactgga tcgagctgcc gacatagcgt tcctgcagcg gacgaaccgg
    12721 caaatagact gcgttggcgc cgcgcagctt ggcttgcaga atacgatagc gttcgttcca
    12781 tcgctcaatg ttgttttcga gctcggcaag ttgcggtctg aggatcgaag cccggagatt
    12841 gtccattcgc ccggagaaat tcggcgtgtc gaggcggatg tcggcgaaga ccgccgggtc
    12901 tggggcagcg ccgtgccgct cgtagagcat gtacgatcca gacattatga tcgcccgggc
    12961 catgaactcg gcatcctcgg aggtgagtag tccgccttct ccggaattca tatgcttgta
    13021 cgtctgggtg ctgaaacagc cggcgaggcc aaaggaccca cttttgcgcc catcccaaga
    13081 cgcccccatc gtatgtgcgc agtcttcgat cagaatcaga ccgcgccgga cgacgagtgc
    13141 caggagggca tccatgtccg gcatatgccc gcgcatgtgg gacagcagca ggaagcgagc
    13201 ctttgaagcc gatgccttgc gttcgaggtc atcgagatca atgacgagat cgcgcgtggt
    13261 ctcgacgaaa atggcccgtc cgcccgcatt gacgatagcc cccggtacgg gcgcgagggt
    13321 aaaggcgttc gtgagcaccg gctcgcccgg ttcaagccca gccgcgcgca aggtgatctg
    13381 tagggcatat ccgcccgacg cacaggcgag gcagtagcgg ctgccctgat aggcagagaa
    13441 ttcgcgctcc aacatcgagg cttcagacgt ttccgccacg agggtgttgt agcgatgcag
    13501 gcgacccgag cgaagcactt ccaccgctgc ggcaatgccc tcttcgctga tcggctcctg
    13561 ctgggtgaag ttgcccttga acggccggag cttcatcgag ccgcctccgt tgatcgtcac
    13621 tgggccgcgg ccattgcggc gcgctggccg aaatcaaagg cggcggtata gccgaatagg
    13681 gctgccgacc caccggtatg tacaaacacc acccgctcgc ccttcttgaa atgacccttg
    13741 cgaatcagat caatcagccc ggccgcgccc ttacctgagt agacgggatc gagaagaatg
    13801 ccttcaatct gggcgaacat ctcgatcgcc tcgatggtgc tcgcggcggg aatgccgtag
    13861 ccttccccga cgtagtcgca gttcgcgacg gcgtcctcgc gggcaacgac gccgggacaa
    13921 ccaagctttt ccgccgtggc ggcggccagt ttgaagacgt tttcctcctg cttggccttc
    13981 ggcgcgcgta ccccgatgcc cagaagggga atgttggcgt tgatcgtctt caggccagtc
    14041 accaaccccg cctgcgtccc ggcgcttccg gtcgcatgga cgatatggtc gatcaccagc
    14101 ccccggtcgt tggcctgcga caccagttca agagcgcagt tgacatagcc caacgccccc
    14161 gtcgggttgg atccgccccc cgggatcaca tagaccttgc gcccttctgc acggaagcct
    14221 ttggcaacgg cctccatttc ggcattcatg tccatgccgc cgggccgctt ctcggcggtc
    14281 gcaccatgca ggaaatcgag aaagacgttg ccgttcgtat tgtaattagg atccctcgat
    14341 cccgtccggt cttccagcag aatgtggcag gaaagcccga gcttggctgc aaaggccgcc
    14401 gtttgccgcg cgtggttcga ctgcgtcgcg ccctgcgtca tgaccaggtc cgccccttgg
    14461 gctcttgcct cggccatcag gaattcgagt ttgcgagtct tgtttccgcc ggttgacagg
    14521 ccggtgcaat cgtcgcgctt tatccagatt tcgggcccgc caaggtgacg cgacagccgc
    14581 tcgagaggct cgagaggcgt cggaagatga gcgaggcgaa tcctcggaaa tcgcgcgaga
    14641 tgcattggat acctcctgtc ggaaaaatgc acgattccga tgatgtacgt gagcatattc
    14701 gcttgcaaat gcgaaattgc gggcaaaaaa tgcaatttat gcatgttatt ggaagctggc
    14761 atgcgcctgg aatggattga ggatatcctg gcgattctgc gagcaggctc gttgagccgg
    14821 gctgcggagc agcgctacct gacccagcca gccttttccc ggcggattag gtcgatcgag
    14881 gaataccttg gcgttgaact ggtcgacagg acgcgaaaac ccgcccaact gcgccgatcg
    14941 gtcctggaaa agcagcagcg attggaggaa ttggccgagg gcttgcacaa cctgctctac
    15001 gaattgcgtg agcaggatcg gaagatcgaa aaccgcgtca cgattgccag ccagcacgca
    15061 atcaccacgt ccgtagcccc gactctggtt aagcggttat cgacggacac ggatacgcac
    15121 attcgcctgc gatctgccaa tcgggacgaa tgcttcaacc ttctcgttac aagacaggcc
    15181 gacctgacgc tcacctaccg ttcgcctgac gagcaactgc cactcgaagg ggcctacctc
    15241 gaaaagtgcg acctgggcga ggagcagttg attcccgtct ttgcgaccga cgcgctatcc
    15301 gctctcaacg atggctaccg cgcgggagaa ttgccggtta tcgcctaccc ggccgatgtg
    15361 tttctcggcg aggtgatgaa ccgggagatc tttccgaagc tgcgatccca gtcgttcttg
    15421 agagccaagg cggaaacggc gctgacgctg gcggcgttgc aacttgcact aaccggcgtc
    15481 ggcgtcgctt gggtaccccg ttccctggcg gcaaaggaca tcgcctacgg taatctcacc
    15541 gagctgagcc acattttctc caaggcgcgg ctgacgatcg gggcgatcag actcgccggc
    15601 tcgaaatcgc ctgccgagca ggaggtatgg aatatcatag gcgggcatac cgcatcttcg
    15661 ggcgagcaaa catcggccgc catcggcgaa gcgcgcgcag accgatgccg gatcacctcg
    15721 gcgcccgctc cttcagaata aagtcccacc gaatgttcag ctgccgccgt atcgcccaga
    15781 ccccggccat gtccgggccg atgaaggcga gcgcggcgcc gttccgggcc tcgcaacgcc
    15841 ccagggcgga gcgcaacgcc gccccgggat ggcgacggcc acagcgggtc gctcggcatc
    15901 aaggccaccg gacgcgaggt gcccagtttt gtacaggagt ggcgcccccg aggggactcg
    15961 aaccgcgagc acccggcgcc ccggccgttc ctgctcttgc gcgcggcagc atcgatcggc
    16021 agggctgacg atgtggcgaa gaatcgcgaa ctgcccgcct cggatatccg gggcgggcgg
    16081 ttcgtgtttc tggcgtgtca actgtcgttg gttgcggggg cctgaaacca ccgacagttg
    16141 actctgcggt tataggtgca aatcgagggc ctgaaactac cgatagttaa ctcaatcacg
    16201 ccggccccaa gtcgccagcg caaaaccgcc gaagtagagg tttcgggcta aggatgccgg
    16261 ctcacttcaa gggctaccgc tttactgttt cgagccaccc ggacgatgca ccgcagccgg
    16321 ttccgcccgg cctctggtcc ggattcattt tgaacaagct ccacatcgac gacggctgaa
    16381 agcccttcta tcgatagcaa cgagctgttc cgtatctgca ataccgctgg cgtccgcacc
    16441 agaggtcgcc tcaatcaggt tcgcagccga ggtgatgccg cgcaaaggag ccaagggacc
    16501 gccgtatcgc cggtgggcga ccgatccgtg gagcacccaa gcaccgaaag gcatctccaa
    16561 tctccttccg ttgcgcccgt cccattacta aaagagatag tagagcgtgc gccttgcgtg
    16621 aactgagcca gcctgcgctg atcaggccac gggaaagcaa atccgtctcg gccccggcat
    16681 atccataaga cgacgaaagg atcgggccgt ccgcgacgct gcgcgctaga acgactggaa
    16741 tttctgttgc cgccgcttca agggctgcaa ccattccggg cgcgacatgg ccagcgccca
    16801 tcccgagcac caccaagccg tcggaaccaa cgtccatggc tgcggcgacg agccgcccat
    16861 cgccaccaat atcggcccca agagtgtaga ccgccgccgt gacgccggga tgcgggcgga
    16921 tcaatggcac tcggtagggc tccagccgaa cgagcaggtg cagatgcccc tcgatgatcc
    16981 agcccaacgg cccc
//
LOCUS       DASVDU010000183         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1173553, whole genome shotgun sequence.
ACCESSION   DASVDU010000183 DASVDU010000000
VERSION     DASVDU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173553"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1625
                     /locus_tag="VF226_11970"
     CDS             252..1625
                     /locus_tag="VF226_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEX7074750.1"
                     /translation="MMGRQRADQAQFFYSFHLDERIPASHLLRKINVFVSKALADVHR
                     EMAAFYSHTGRPSIDPELMMRMLIVGYCYGIRSERKLCEEVSLNLAYRWFCRLDLDDA
                     VPDHSTFSKNRHGRFRESDLLRLVFERVVGICLTAGLIKGDGFAIDASVIEADASRYH
                     GVEPEKIDWSSVERPTRAVREYLDTLDSAAEPEAGRKAPKVISPSDPASAWTAKANKR
                     VQFGYGLNYLIDNEHAIIVDVEPTPARTFDEVRSTATMLDRTKARFGIKPKRLAADTA
                     YGTGKFLGWLVDQEIEPHIPVWDMGNRDDGTFGRSDFIFDPEQDQYICPAGKVLKQFR
                     RNYAVARTDSAHDGIRKYRASQKDCQTCELKSRCCPNMPSRNVPRSVHEEARDHARQL
                     AGTPQFERSRNERKKVEMLFAHLKTTMRFERMRLRGLSGARDEFLLAAIAQNLRRMAR
                     LAAAAPA"
BASE COUNT          443 a          675 c          668 g          404 t
ORIGIN      
        1 cagggtcgcc aattcgccca ggtcgccgtt gtgaacccga gcgggattct cgcctaaagc
       61 gccaaagccg gcaatcgaca ccagtcagcc gagcggatgc cggcgagaga gtcggctgtc
      121 gggaggtgaa ccggaccgac cgcagaaagt gctcggatgg ctctgtttga ccgatgccgt
      181 tgaagaactc ggttgagttg gtggcgtgag cgtgattccg tctttctcgt gacttgcggg
      241 gggacgaatc gatgatgggg cggcagcggg ctgatcaggc gcagttcttc tattcgtttc
      301 atctggatga gcgcattccg gcctcgcatc tgctgcggaa gatcaatgtt ttcgtcagca
      361 aggcgctggc agacgtgcat cgcgagatgg cggcgttcta cagccacacg ggccgacctt
      421 cgatcgatcc cgagttgatg atgcggatgc tgattgtcgg ctactgctac ggcatccgtt
      481 cggagcggaa gctgtgcgag gaggtctcgt taaacttggc ctatcggtgg ttctgccggc
      541 tcgatctgga cgacgcggtc cctgatcatt cgactttctc gaagaaccgt catggccgct
      601 ttcgcgagag cgatctgctg cgcctggtgt tcgagcgcgt ggtgggcatt tgccttactg
      661 cgggactgat caagggtgac ggcttcgcga tcgacgccag cgtcatcgag gccgacgcca
      721 gccgctatca tggcgtcgag ccggagaaga tcgactggag ctccgttgag cggccgacac
      781 gtgccgtgcg cgagtatctc gacacgctcg acagcgccgc cgaacccgag gccgggcgta
      841 aagctccgaa ggtgatctcg ccgtccgatc cggcgtcggc ctggactgcc aaggccaaca
      901 agcgcgttca gttcggctat ggccttaact atttgatcga caacgagcat gcgatcatcg
      961 tcgatgtcga gccgacgccg gcgcggacct tcgatgaggt tcgatccacg gcgaccatgc
     1021 tggatcgcac gaaggcgcgg ttcggcatca agccgaagcg gctcgccgcc gacaccgcct
     1081 atgggaccgg caagttcctc ggctggttgg tcgaccagga gatcgaaccg cacattccgg
     1141 tttgggatat gggcaatcgg gacgacggaa cgttcggccg atccgacttc atctttgatc
     1201 ccgaacagga ccagtacatc tgtccggcgg gaaaggtgct gaagcagttc cggcgcaact
     1261 atgcggtggc gcgcaccgac agcgcccatg acggcatccg caagtaccga gcatctcaaa
     1321 aggactgcca gacctgcgag ctcaagtcgc gatgctgtcc aaacatgcca tctcgcaatg
     1381 tcccgcgcag cgtccacgaa gaggcccgtg atcacgcccg tcaacttgcc ggaacgccgc
     1441 agtttgagcg atcccggaac gaacggaaga aggtcgagat gctgtttgcg catctcaaga
     1501 ccacgatgcg cttcgagcga atgcgtctcc gagggctgtc aggcgccagg gatgagttcc
     1561 tcctcgcagc catcgcccag aacctcaggc gcatggctcg actagcggca gcagcaccag
     1621 cttgagcgcc cagtgcgggc caaggcccac aggagcgcca tccaaaagct ccgctcacgc
     1681 catcagcggc cagacctcga cgctcaatca aacgagtttt tcaacggcat cgacccaatc
     1741 cggaagtcga gcgcgacaag cacttcaatg gaagctcact acagtgtcgc ggagccgcgg
     1801 cggcgggctg tcataccgca tctcggtcag cacgatcttc cggcgcggca tgaatgggaa
     1861 ggggccgtct ccttcccata gcggacagcg aatggccgag gacgaacaca gagcaatcta
     1921 cgtaaccacg tctgaccttc cccggagccg tgatcagcgg tcgccgattt gccctacatc
     1981 ggtcgtacgc cggcgcgcag gtgacgccta tcactcccac ccctcatcaa atggaaaccg
     2041 cctcgcccag aactccttgg gacgtgtggc ctcggtcgga actgagcccg atcccgctgt
     2101 gcctagactg gcacataccg ctcctggttg acctttctct ggtccagata aaaacaccag
     2161 gaactcacaa gactcgtata ctaccagcgc
//
LOCUS       DASVDU010000184        31665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1001740, whole genome shotgun sequence.
ACCESSION   DASVDU010000184 DASVDU010000000
VERSION     DASVDU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 31665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31665
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001740"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF226_11975"
     CDS             <1..247
                     /locus_tag="VF226_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HEX7074751.1"
                     /translation="MSPSAEVQGHSPDQRVTVARKRNVKIRELRPVGNYAVRIVFDDG
                     HDTGLYAWSYLQILGREKEQRWAEYLRELEQKGLSRG"
     gene            394..1437
                     /locus_tag="VF226_11980"
     CDS             394..1437
                     /locus_tag="VF226_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7074752.1"
                     /translation="MRDGGEPADEIVLPYETPPAPGEAIEIAPGLLWARMPLPVRLDH
                     VNIWLLDDGEGWSAVDCGLDTAETREAWERLAAGPLKGKPVRRLVATHGHVDHVGLAG
                     WITQRFNAPLHATLAAWLWARVTHAGRDAPTSPQALEFLRSHGCAPEHVAAYARNRGP
                     LLKLFSDPPKAHVRLRDGDVLRMGDRKWRVLTADGHADEHASFIGMDDRIVIAGDQVL
                     QRISPVVGVFSGEPLADPLSDYLASLDKFRGLPSDALVLPSHGLPFRGLDARLDQLAQ
                     HHQQRLGIVHDALDQPRTATECAHVLFARAMAEGQWFLALAETLAHLHRLVTVGAAQL
                     QTDSGGIIRFART"
     gene            complement(1469..2263)
                     /locus_tag="VF226_11985"
     CDS             complement(1469..2263)
                     /locus_tag="VF226_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074753.1"
                     /translation="MIRVEGLTVTFHRGTPMETKALRGLDLEIPTGQFVTVIGSNGAG
                     KSTLLNAVAGEVVPQSGRILIDSADVTRWPAPRRSALVARVFQDPLAGTCETLTIEEN
                     LSLAAARGRRRGLRLAIDRRSREEFRSRLRSLGLGLENRLGDKVGLLSGGQRQAVSLL
                     MATLCPSQVLLLDEHTAALDPKTADFVLNLTNRIVTEQRLTTLMVTHSMRQALDMGER
                     TVMLHEGKVVLDVSGEARARLDVPDLLAEFSRLRGRELADDSLLLD"
     gene            complement(2260..3174)
                     /locus_tag="VF226_11990"
     CDS             complement(2260..3174)
                     /locus_tag="VF226_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7074754.1"
                     /translation="MNLVAFLGAIEIGLVFGLVGLGVFLSFRVLDFPDLTVDGSFPLG
                     AALSASLIVSGWDPYTATGAAIIGGCFAGLVTAFLNIRFGILHLLASILTMIALYSIN
                     LRIMGRPNVAIISEPTILSSFDGLGLAMLYVKPIVAGIVAIAAILLVARFLASEYGLA
                     MRATGVNPRMARAQGVDTRHTTYLGMALSNGLVALAGALFAQMNGFADVTLGTGTIVV
                     GLAAVIVGEALFGTRSVLVWAAACVAGSVVYRLAVALALNSDFLGLQASDLNLVTAIL
                     VTVALVLPGARSNLKSMFASARWGRAPS"
     gene            complement(3232..4185)
                     /locus_tag="VF226_11995"
     CDS             complement(3232..4185)
                     /locus_tag="VF226_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7074755.1"
                     /translation="MGCAALAFGSTGLVVSAAAQEKVFVAVTAIVEHPALDAARDGVR
                     DELKANGYEPGKNLRFEYESAQGNPTTAAQIARKLVGEGPNVIVPISTPSAQAVVAAT
                     KDIPVIFTAVTDPVGAKLVTDAKKPGGNVSGVSDLSPIRKHLELIQQITPNAKRIGVP
                     HNPGEANSLSLLKLLKQEAGPLGLQIVEAPATRSSDVLSAAQSLIGKADAIYIPTDNT
                     IVSALEAVVRVGTENKIPVYAADTDSVPRGAMAALGFNYYDVGRQTGKMVLRVLKGEK
                     PGDIPVEGVEITELHLNVSAAEKMGVTIPEAVLATAKNVVK"
     gene            complement(4314..4826)
                     /locus_tag="VF226_12000"
     CDS             complement(4314..4826)
                     /locus_tag="VF226_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074756.1"
                     /translation="MLGSFLILLAVTVPVAIYAWRKDQFIMARASMFARQEIVRIFVR
                     LPFALVAASCIAELVPEQTIAAVLGADSGLLGIAAASVLGGFMPGGPIVSFPIAILFA
                     HQGAGGPQVIALITGWSVYAFHRVISYESPMMGWPFVGLRLACSWFVPPLAGIIGGLL
                     ASTFGLSINL"
     gene            complement(4826..5440)
                     /locus_tag="VF226_12005"
     CDS             complement(4826..5440)
                     /locus_tag="VF226_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX7074757.1"
                     /translation="MNDAPHPAPHSQDESGAGKSPPPVFDRAAWFFVLLAGASALLVL
                     LLEGKDALTRAGTQSLTLLITVTPMIVIGLFLGGLVKELSDPDRIAPVLGPRSGWRGL
                     LLATALGAATPGGPFAAFPIVYALALAGADIGAVVTYLTAWSVLGLHRLVIWELPLLG
                     PDFVLARMLACLPLPVVAGLFARLLMHFIPDLPIGRMRASGGDR"
     gene            5761..6579
                     /locus_tag="VF226_12010"
     CDS             5761..6579
                     /locus_tag="VF226_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7074758.1"
                     /translation="MRVFLSLFLPAATAIFSLLALISPAHAVAPDQCLALADTNPRPV
                     IERVSLRLAQLKAAEVRVTYIGHSTFLIESAGGVRIATDYNDYVRPAVVPDIATMNRA
                     HNTHYTPSPDPRIPHVLRGWSEGGAAIHDLSLLDVRVRNVPTNIRTWDGGTMAYGNSI
                     FVFEIGGLCIAHLGHLHHTLTPQQLAQIGQMDVVLVPVDGSYTLDLEGMLEVLKALKA
                     PLMIPMHYFSGATLNRFLARVEDSFEIVRHEVPTIVISRQTLPTTPQVLVLPGR"
     gene            complement(6583..7677)
                     /locus_tag="VF226_12015"
     CDS             complement(6583..7677)
                     /locus_tag="VF226_12015"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7074759.1"
                     /translation="MSLTRREMLSISLGAVLLPHLVQPAEARRFVNRTGLKRGQFVWE
                     PSQPHDGPVTIVASLADRLVHVYKAGVLVGISTCHVGLRRRRTPSGVFAISGIDDSEA
                     DDDRRSWSGIALHASGVQRYPASPGCIRVPAAFARLLQEIVVPGTAVILAPQRTVPMD
                     VVHSGGLFPIVPVYEAANPMVRPVAQRQGHQLLTGTPEAGPVAVVISRASRSAILLRN
                     GELEHVARVRFVQPNSRIGTHVYSLTGTAPAGEGLVWLAFGIGRSRRERHLVSWHGDE
                     LLDQISFEDRASALTMSRALHAGSILVVTDEPATSKKHTTPRNFILISAVSAEPRVRH
                     RSSRRRRRRRVRVVRRRRQNWAEALINSWR"
     gene            complement(7891..8172)
                     /gene="infA"
                     /locus_tag="VF226_12020"
     CDS             complement(7891..8172)
                     /gene="infA"
                     /locus_tag="VF226_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7074760.1"
                     /translation="MAKEEVLEFEGVVSEVLPDARCRVLLDNGHEVIAYTSGRMKKNR
                     IRILAGDRVTVEMTPYDLTKGRINFRHKDTRATPPPPGSGGFRPHHRRR"
     gene            complement(8262..8468)
                     /locus_tag="VF226_12025"
     CDS             complement(8262..8468)
                     /locus_tag="VF226_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7074761.1"
                     /translation="MATGTVKWFNGQKGYGFIQPEEGGSDVFVHISAVERAGLSTLRE
                     GQRVSYELERGRNGKTSAVNLRVS"
     gene            8710..9135
                     /locus_tag="VF226_12030"
     CDS             8710..9135
                     /locus_tag="VF226_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074762.1"
                     /translation="MMDFRKLGLGFLAGCLAVLTAHQLTVYGLSAAGLISAQAWSKEP
                     AGLFGVPAVLNSTFWGGVWGSIFALSADRLPGGSLWSKGLVFGLIFPLVIGAWLIVPL
                     IKGLPFFAGFSAPRLLAGALIHAAYGAALGAFYGLLTRR"
     gene            9281..9622
                     /locus_tag="VF226_12035"
     CDS             9281..9622
                     /locus_tag="VF226_12035"
                     /inference="COORDINATES: protein motif:HMM:NF012689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7074763.1"
                     /translation="MTARQAGKMTVEEAGRKGGEATSSVHGREFYEEIGQKGGAARRG
                     QLGREGYSALGRKGGEATSNKHGSGFYAEIGRKGGETVSRNREHMSQIGRKGGEARAE
                     KGVEHRARERQ"
     gene            9641..9847
                     /locus_tag="VF226_12040"
     CDS             9641..9847
                     /locus_tag="VF226_12040"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX7074764.1"
                     /translation="MARGVGGVSPANLQKYLAGISYPASKDDLIWTARRNSAPNEIME
                     VIERLPGDKFRGPEEVQRAYGDIT"
     gene            9909..10268
                     /locus_tag="VF226_12045"
     CDS             9909..10268
                     /locus_tag="VF226_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7074765.1"
                     /translation="MAKSRSDGGKMTVEEAGRKGGAATAQARGREFYKEIGRKGGEAR
                     RGKLGSEGYRELGRKGGAATSQKRAPVRESEHLAGISQTQQAISNEETTPGLSETGDI
                     ALEEEHQIDPAEPVRGT"
     gene            10593..11369
                     /locus_tag="VF226_12050"
     CDS             10593..11369
                     /locus_tag="VF226_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003546546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7074766.1"
                     /translation="MPADCERQWAMWEEIIFYIIVGFAAQVIDGALGMAYGVTVSTVL
                     LSTGVPPAVTSACVHAAEVFTTGTSGFAHWCAGNADRRLILRLVLPGMIGGAIGAYVL
                     SNVPGEMIRPWVSGYLLVLGFIILGKAVRYRPYHPDPPKAVAPLGFIGGMLDAMGGGG
                     WGPLVTSTLLGYGSAPRTVIGSVNMTEFFVTSTISITFFATIGLSLWPIIAGLVLGGV
                     LAAPLAALVVRYLPARVLMVIVGCVVVGLSSWTILRVMMT"
     gene            complement(11446..12729)
                     /locus_tag="VF226_12055"
     CDS             complement(11446..12729)
                     /locus_tag="VF226_12055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7074767.1"
                     /translation="MSGLPAVIDQAAVVIIGGGVIGCSAAYHLAKRGIADVLIIERNQ
                     LASGATARAAGLVCHARSDTSTIHMVKRTVAAISELETLLEEKIDFRQVGSLRAILSD
                     SRWNELQTMEECLAAAGVDFEAVDAAAAHGLCPWLELDAARRIIFIPADGYIDGARLG
                     TAYSAAARRLGARVRRGITAKSLLANSGRVTGVQTDQGIIRADWVVDAAGVWGAEVAS
                     WLGWGFAAAPTRSHYWITVPDGSGAPGQPNVHLPDMRAYLRSEVGGLLIGLQEPKSLT
                     FNPLELSADMNDMLLTNEERDLDLLVEGANALRTVIPKIDEWRFAHHIAGLSNYTPDG
                     KFVLGPIPGYERFLLAGGCCGSGVAASGGFGEIIADLISGTEPSIDITVYRADRFGLV
                     DPTSEAFRERCAAARAGKSRGHADPSATSESQTVL"
     gene            complement(12749..14005)
                     /locus_tag="VF226_12060"
     CDS             complement(12749..14005)
                     /locus_tag="VF226_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074768.1"
                     /translation="MSQTKSVAPRIMVVGAGIVGVCCAVHLRREGHSVSLLEAEEPAA
                     GASRGNAGALSPGSCIPLSMPGLLKDVPGWLIDRNGPLVVRPSYFLQVMPWLFRFIAA
                     GRATRIPQIVDALHALHSPVFDSYMPLLQAAGIEYLIRRTGSLTVYRHRRGLESAMSG
                     WKLRQERGVDFEILDQGAIHDIAPALADKFECGVFQPQHGYVADPQSLVQRLAVHFTA
                     CGGELLIGRAARVEPQNEAVTIWLEDGRRLEADRVVIAAGASSKPLLEGLGISIPLET
                     QRGYHLHLPEPGVELPLPVSFADAKFYATPMEHGLRLAGTVEFARPSAKPDFRRARQL
                     GTMAMEWLPGLRLANASEWMGRRPCTPDSLPVIGPLPTDRRILLAFGHGHNGMTSAPT
                     TGRLIADLMADRKPFIDVSPYRPDRF"
     gene            complement(14032..15747)
                     /locus_tag="VF226_12065"
     CDS             complement(14032..15747)
                     /locus_tag="VF226_12065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEX7074769.1"
                     /translation="MAATSETYIGRVSRSHARGVPFRLLFSPTLILTGLALFLILFAV
                     APLILLFWRSLQNADGTFIGLANYVAFFSQPVAAEVLWNTLFVAIVSTIITVGVALLF
                     ALALAHTCVPFKDFFRSVALLPLLTPSLLSAMALTQLFGSQGYLNSLMMGQSIYGPIG
                     IVIAMSVAHFPHVSIILSAAIALSDARLFEAARALRARPLRIFLTVTLPSMKYGLVSA
                     GIASFTLCVTDFGIPKVIGGQYSMLATEIYKQVVGQQNFQIGAVISILMLIPAAFAFI
                     IDRRVRRRQAATLTSRAVPFVPSPSLLWDRMALVLCLAITVIMLSMIFVPGYASFIKF
                     WPYNLELTLRNYEFHRFAGGGWESYYNSLKLALLTATIGTAVIFVGAYLSEKSPAPAW
                     LRSAYHMLGVFPLAIPGLVLGLSYVFFLNDPTNPLEVLYGTMAVLVISTIIHYFTVSH
                     LTAITALRQLDNEFELVSDSLKVSRLRTFFVVTLPLCLPAILDIWIYLFLSGMTTVSA
                     AVFLYSHRTALASIAVINMDDAGEYAAAAAMAMVIVATCIAARCVHLLATRGLARRAQ
                     AWKAR"
     gene            complement(15790..16827)
                     /locus_tag="VF226_12070"
     CDS             complement(15790..16827)
                     /locus_tag="VF226_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7074770.1"
                     /translation="MRPLFGALTTAAIVALGLGFASAAAQAKTELTVYTAYENDDLAA
                     YKEAFEAAHPDIVVNWVRDSTGVVAAKLLAEKDNPRADAVWGLAVTNIDVLKARGVLM
                     PYKPAGFEKIPEKFRDPADPPYWFGNSAWICAIIYNEIEGKKRGIPAPKTWSDLTDPV
                     YKGQIVMPHPASSGTGYMYVTAWIQTYGEEEAWKLMDGLHENISRYTHSGSAPAVIAA
                     RGEQVIGLAFELRGSRLKQDGAPIELILPEEALGWDMNAMAIVNGTKKLDAAKKLADW
                     AASEEAMKLYGATRSVVAMPGLAKEIPYVPKDIGERIMKQNFAWSAENRERILAEWEK
                     RYGGKAEPKKK"
     gene            17471..18364
                     /locus_tag="VF226_12075"
     CDS             17471..18364
                     /locus_tag="VF226_12075"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7074771.1"
                     /translation="MNFLYLRAFHAVASERSFTRAAEVLHVSQSTLSWQVKALEERYG
                     VRLLDRRGKQVVPTEVGKRTLQLCREVFRLQDEIEQTLNETQKLQTGRLKVGADGPRH
                     VLPVLAHFMRLHPNVSISLTTGSAKKVMSDLLNYETDVAIVAAERPRETQLHMVHFQT
                     YPLVAYVPRKHPWRRRKSVKPIDFHGQQVIMREPTSLTRHKFLRSLHRAGAVPFMVIE
                     IDNREATREAVALGLGIGVMGSSEFHGADPRCVSLSIEDPELHFTEYVACLEKRRDMR
                     TIREFFRIAEMFATPPPAASN"
     gene            complement(18361..20163)
                     /gene="lepA"
                     /locus_tag="VF226_12080"
     CDS             complement(18361..20163)
                     /gene="lepA"
                     /locus_tag="VF226_12080"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX7074772.1"
                     /translation="MTDTSLIRNFSIIAHIDHGKSTLSDRLIQLCGNVTDREMKEQIL
                     DSMEIERERGITIKAQTVRLDYKHKDGKTYQLNLMDTPGHVDFAYEVSRCLAACEGAI
                     LVVDASQGVEAQTLANVYQALDNDLEIMPVLNKVDLPAADPERVKQQIEDVIGLDASD
                     AIPISAKTGLNVEAVLEGIVERLPPPKGDRNKPLKALLVDSWYDAYLGVVVLVRIMDG
                     VLRKGQRIKLMASGGSYQIERVGVFRPKQEMLTELGPGEIGFFTAAIKQVADTRVGDT
                     ITDEKNPTAEPLPGFKPVQPVVFCGLFPVDAADFEDLREAMAKLRLNDASFSYEMETS
                     AALGFGFRCGFLGLLHLEIITERLEREFGMDLITTAPSVIYHVYLTDGSRLELHNPAD
                     MPDPVKIDHIEEPWIQATILVPDEYLGAVLKLCQDRRGRQKNLTYAGNRAMLVYELPL
                     NEVVFDFYDRLKSVSRGYASFDYQIIGYEENDLVKMSILVNGEPVDALSIIVHRSRAE
                     QRGRAMCEKLKDLIPPHLFQIPIQAAIGGRIIARETIRALRKDVLAKCYGGDVTRKRK
                     LLEKQKAGKKKMREFGKVEIPQEAFIAALKMEEN"
     gene            complement(20216..21037)
                     /gene="phnE"
                     /locus_tag="VF226_12085"
     CDS             complement(20216..21037)
                     /gene="phnE"
                     /locus_tag="VF226_12085"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX7074773.1"
                     /translation="MPVVATAAGQPVTWRYRTRLRQLAVWLGWLALVALTVFCWRVMT
                     ANTIWAFVEDAPRQAADIFGRMLPPRWEYANKLWLPLWDTLNIATLGTALAILLGTPL
                     GFLAARNTTPSILFVRPIALLIIVSSRSINSLIWGLLLVAVFGPGVFAGILAIALRSI
                     GFIGKLLYEAIEEIDETQVEAVRATGASPAQVMAYGMVPQIMPAFATICVFRWDINIR
                     ESTVLGLVGAGGLGLALEASLNVLAWPQVTVILLMILATVVLSEWITARVRAAII"
     gene            complement(21040..21858)
                     /gene="phnE"
                     /locus_tag="VF226_12090"
     CDS             complement(21040..21858)
                     /gene="phnE"
                     /locus_tag="VF226_12090"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX7074774.1"
                     /translation="MDAETLPSAGLPRTWHPPRTIETAWVRWAAVLGVLLYLVLAFSS
                     IEVNWARVVDGVARAGRLFAGFLTPDFVSRWGDIQAGMIESLTMTVTSTIAGILLSVP
                     IALGAARNIAPRWLYGICRGIVALSRTFQEIIIAIFFVAMFGFGPLAGFLTLSVATIG
                     FLAKLLADDIEEIDPVQVEAIRATGASFWQVIDYAVSSQVKPRLIGLSLYRVDINFRE
                     SAVVGIVGAGGIGATLNTALDRYEFDSAAAILMLIIAIVMACEYFSSFIRRWTQ"
     gene            complement(21860..22711)
                     /gene="phnC"
                     /locus_tag="VF226_12095"
     CDS             complement(21860..22711)
                     /gene="phnC"
                     /locus_tag="VF226_12095"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX7074775.1"
                     /translation="MLRIEALTKRYRTGDAALRGVSFSVGSGEIVGLIGPSGAGKSTL
                     IRCVNRLVEPTSGKVFLSEEELTALGKSALRRARRRIGMIFQEYALVERLTVMENVLS
                     GRLGYTGFWTSYFRRFPQADVERAFAVLERVGLLDHVDKRADALSGGQRQRVGIARAL
                     VQEPDLLLVDEPTASLDPKTSRQVMRLITELCAERRLAAIVNIHDVALAQMFLPRIVG
                     LRAGEIVYDGPASGLTPEVLTLIYGEEDWSKTSAAAEGDGEAAEGRYRDALDHHAHDR
                     DLTAAMR"
     gene            complement(22744..23706)
                     /gene="phnD"
                     /locus_tag="VF226_12100"
     CDS             complement(22744..23706)
                     /gene="phnD"
                     /locus_tag="VF226_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7074776.1"
                     /translation="MKAFLNRSFGFLAVLTIAAAILSAPASALDPRFQDNDGDLVADT
                     PTDPSQLIDPPTLIFAYTPVEDPAVYAKVWDGFLKHMEKVTGKKVQFFPVQSNAAQIE
                     AMRAGRLHIAGFNTGSNPLAVACAGFRPFAIMASADNSFGYEMEIITYPGSGIEKVED
                     IRGKKLAFTAETSNSGYKAPSALLKSEFGMEAGRDYEAVFSGKHDNSVLGVANKDYPA
                     AAIANSVMQRMIERGVVKAEQIKSIYKSQTFPTTGYGVVYNLKPELQEKIKEAFFTFP
                     WEGSELQKEFSRSGEAKFIEITYKKDWEVVRKIDSAMGVKYTCK"
     gene            complement(23831..26308)
                     /gene="hrpB"
                     /locus_tag="VF226_12105"
     CDS             complement(23831..26308)
                     /gene="hrpB"
                     /locus_tag="VF226_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HEX7074777.1"
                     /translation="MRFDAPLPIDAVLQELADALSRGTAAVLVAPPGAGKTTRVPLAL
                     MGADWLRGRKILVLEPRRLAARAAAERMAQTLGETVGETIGLRARLSSRIGPRTRVEV
                     VTEGVFTRMILDDPALEEVGAVLFDEIHERSLDADLGLALVLDAKSALRDDLRLLVMS
                     ATLDGARVAELLGDAPVIHSEGRAFPVETRYVGRDPAQRIEEQMAAVIREALRDERGS
                     VLAFLPGQREIRRVAERLGEQLDDPAVQIFALHGGLDKHAQDEAIAPAESGRRKVVLA
                     TAIAETSLTIEGVRIVIDSGLARVPRYEPGVGITRLVTVRASQASVDQRRGRAGRTEP
                     GVCYRLWDEPATASLPLFTEPEIRNADLSGLLLDCAAWGVTDPRRLQWLDPPAEAPLS
                     AARAELEALGALDAEGRITGLGRRLRALPLPPRLARMVLSAAGFGAAEQAAEMAALLV
                     ERGLGGSDTDLEHRLAIFRADRLRRARDMRDLARGWARAAMREAAGQSLTAGSEGASV
                     AALLALAYPDRIAKARGAPGQYLLANGRGALLDPTDPLARSEWLVVAELQGAAASSRI
                     LLAAATDEAEVHAIARDRIVETDEITFDRDAGALRARRVRRLGAIVLASEPRPVPPEA
                     AAEKLAAGLAEIGIDRLPWSKAQKQLRDRVQFLRTMTGENWPDLSDSALAAKAREWLA
                     PFIQGKTRLDEITSDDLGAALDGLLPWELRRRLDAEAPTHFVSPAGHSHPIDYSGPDA
                     PSLHIRVQELFGLDRHPSIAGGRLPLTLHLLSPAHRPIQITRDLPGFWRGSWAAVKAE
                     MKGRYPKHPWPDDPIRAAPTTRPKPRS"
     gene            26460..27416
                     /locus_tag="VF226_12110"
     CDS             26460..27416
                     /locus_tag="VF226_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX7074778.1"
                     /translation="MRTFAELSEKEILALAISLEEEHGRIYDEYAHALADNFPASAKV
                     FKEMAAEEDEHRRWLIDLYRQKFGDHIPLIRSQDVKGFVKHQPIWLVRPLGLDKVRAQ
                     AEAIEYETRRFYEKALERASDASVRKLLGDLAAAERNHISLAQQLGEAHLTADARAQE
                     AEAQRRMFVLQVVQPGLAGLMDGSVSTLAPLFAAAFATHNTWETFLVGMAASVGAGIS
                     MGFAEALSDDGSMTGRGRPWIRGVITGLMTTLGGVGHTLPYLIADFGSATALAIAVVL
                     VELWAIAWIRARYMDTRFLQAAFEVVIGGLIVFAAGILIGSA"
     gene            27570..28139
                     /locus_tag="VF226_12115"
     CDS             27570..28139
                     /locus_tag="VF226_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7074779.1"
                     /translation="MDEKAEGSRKRLSDRQGSTAFATRLKQLRVLRGYRTARAFAQAL
                     QIDENRYTRWERGEVEPSVAMLAKMAEVLNLPVDILVSGGDVAVGLPSPMAVRDFGPR
                     VASSHGRPEHPGMQEEASGFTFERTPEELSQKVLAASRTYLMSLLERRVAGMSQNEID
                     SLIAKLTTAPDSDLIPLETKEPPDKPSSR"
     gene            28320..28814
                     /locus_tag="VF226_12120"
     CDS             28320..28814
                     /locus_tag="VF226_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX7074780.1"
                     /translation="MKGPEISLKHLQRAITMELTTVNQYLLQAHQLANWGIDRLSERM
                     FGEIEEEREHANKYIERMLFLEGEPNVRDLDEIVKPESVRQIFETQYKMELDARAYYD
                     KAARECQDAGDSGTFELFVSILKDEESHINFLEDQFDLMKLMGDQLYIARQVSSVAKD
                     EDDL"
     gene            29078..31285
                     /locus_tag="VF226_12125"
     CDS             29078..31285
                     /locus_tag="VF226_12125"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX7074781.1"
                     /translation="MTRSRPTSSGPIQPRPSRGADARRAQKPRSTTLQTALLWFVVSV
                     AALVVAATTFLIIATPTDLIEDRIAQEVHQRTGRNLVVAGGSSLSILDGLGIKLHDVS
                     LASPPDMGGGPMARVAEVDVKIPFWPLIRGELRVDRLVLRQPELSLRVDSDGRRNWKF
                     NRPVRKAGPQRTGAGGGTVGSALAGTRTPLPLANVLIEDGIIRYANEKSDVREEISGL
                     DLQVSASSLDSRLEATGDLTIRDQEFEFRGSLSSLSSLLAGAPSEFAIDLKGQPLKAK
                     YEGTILTSPATQTAGTVAVQSSSIRALADWLDSSAFDEHKDEPLVVSGRLELTPAAVT
                     LSEADVRLGDTTVDGLAILESRSGTRPRLSADLHVSQLDLGYWLTPTGKAASSRVDGG
                     GNSGIGDESEPQSIDDLLRGTQTDGTQVRGFIARHGWSDVPFDYEKLGAIDADLKLLV
                     DQVTYRKVKAGTARITARLADRRLDASINEMELYDGTGRGTVQLDATGSVPTIQAELQ
                     IDNVSAFDLLRDAAEFDWVDGKGRISIAITGQGQTEREVVETLNGKAEFAFRNGALVG
                     VNIPGIIESLQRGRIPRLERNMAERTKFSALDATFSIENGTAQNRDLRLESELIHVSG
                     AGKANLPDRTLDYTVRPKLLSGRKANGGESSSGLELPIRITGSWDHPTYAAEIDSVLK
                     NPDDVVEAFKEIGKQLKGKNLSEALDSLLGGNDGDDGRKKPKARELLRQFLKP"
     gene            complement(31409..>31665)
                     /locus_tag="VF226_12130"
     CDS             complement(31409..>31665)
                     /locus_tag="VF226_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2853 family protein"
                     /protein_id="HEX7074782.1"
                     /translation="GIVRYCGIALQKRDSSLVSFSDKEELARVRENFLKKKLGLTLDD
                     AELDKAISAVGERMKGARAKNRVTVYYLLAEHFGKLSAFQ"
BASE COUNT         6121 a        10041 c         9882 g         5621 t
ORIGIN      
        1 catgagtccg tccgccgagg tgcaaggcca cagcccggat cagcgggtga cggtcgcacg
       61 caagcggaac gtcaagatca gggagctgcg gccggtcggc aactacgccg tgcggatcgt
      121 gttcgacgat ggtcacgaca ccgggctgta cgcctggtcc tacctgcaga tccttgggcg
      181 ggagaaggag cagcggtggg ccgaatacct gcgtgagctg gagcaaaagg gcctgagccg
      241 cggctgagat cagctctgcc ttttgtccct tcaggtctcc gttcggcgcg gacctcctat
      301 gcgcacaggc cttcctgtct tgcgcgggca tcgttcgatt gcttcagttc cggtcgatcc
      361 aagatcattc gccatcacgt agaggacgaa cctatgagag acggcggcga gccggcggac
      421 gagatcgtgc ttccttacga gacgccaccc gctccgggag aagccatcga gattgcaccc
      481 ggcctgctct gggcaagaat gccgctgccc gtccgcctcg accacgtgaa catctggctt
      541 ctcgacgacg gggaaggctg gtcagccgtc gattgcggcc tcgacacggc cgagacacgc
      601 gaagcctggg agcgccttgc cgccggaccc ctcaagggca agccggtgcg gcgcctcgtt
      661 gccacgcatg gccacgtcga ccacgtcggt ctggccggct ggatcacgca gcgtttcaac
      721 gcgcccttgc atgccacgct cgcagcctgg ttgtgggcgc gggtgaccca tgccgggcgc
      781 gatgcgccga ccagcccgca ggctctcgag ttcctgcgca gccacggctg cgcaccggag
      841 cacgtcgcgg cgtacgcccg gaaccgcgga cccctgctga agctcttcag tgatccgccc
      901 aaggctcacg tccgcctgcg cgatggcgac gtcttgcgga tgggcgatcg gaaatggcgc
      961 gtgttgacgg cagatggtca cgccgatgag catgcctcgt tcatcggcat ggacgatcgg
     1021 atcgtcattg cgggcgacca ggttctgcag cggatttcgc cagtcgtcgg agttttctcc
     1081 ggtgagccgc ttgccgatcc tctatcggat tacctcgctt ccctcgacaa gtttcgcggc
     1141 ctgccctccg acgcgctcgt tctgccgtcc catggtcttc cgtttcgagg cctcgacgcc
     1201 cgcctcgatc agctcgccca gcatcaccag cagcgcctcg ggatcgtgca tgacgcgctc
     1261 gaccagccgc gaacggcgac ggagtgcgcg cacgtgctgt tcgccagggc gatggccgag
     1321 ggccagtggt tcctcgcgct cgccgaaacg ctcgcccacc tgcaccgtct cgtcacggtc
     1381 ggcgcggcgc aactgcagac ggatagcggc gggatcatcc gctttgcgcg gacatgagcc
     1441 ctggcggcac ggtttcaagg ttgcagcatt agtcgaggag caggctgtcg tcggcgagct
     1501 cgcggccacg caggcgggaa aactcggcga gcagatcggg aacatcgagc cgtgcccgcg
     1561 cctcacccga gacgtcgagg acaaccttgc cctcgtggag catcaccgtc cgctcgccca
     1621 tgtcgagcgc ctgccgcatc gagtgcgtga ccatcaatgt cgtcagccgc tgctcggtca
     1681 cgatccggtt cgtgaggttc aggacgaaat ctgcggtctt tggatcaagg gccgccgtgt
     1741 gctcgtcgag cagcagcacc tgcgacgggc agagcgtcgc catcaggagg ctgacagcct
     1801 ggcgctgccc gccggacagc agaccgacct tgtcgccgag gcggttttcg agaccaagtc
     1861 cgagcgacct caaccgcgat cgaaactcct ctcgtgaacg ccgatcaatc gcaagccgca
     1921 ggccacggcg ccttccgcgc gccgcagcca gcgacaagtt ttcctcgatg gtcagggttt
     1981 cgcacgtgcc agccagcgga tcctggaaca cgcgggcgac cagtgcgctg cgccgcggcg
     2041 ccggccatcg cgtcacgtcc gcactatcga tcagaatgcg ccctgattgc gggacgacct
     2101 cgccggcaac ggcattgagc agggtcgact tccccgcgcc gttggacccg atgacggtga
     2161 cgaattgccc cgtcggaatc tcgaggtcga gccctctgag ggccttcgtt tccatcggcg
     2221 ttccgcgatg gaaggtgacc gtcaatccct cgacgcggat catgacggcg cccgccccca
     2281 acgggcggaa gcgaacatgg acttgaggtt gctccgcgcg cccggcagca ccagtgcgac
     2341 ggtcacgaga atggcggtga cgagattgag gtccgaggcc tgaagcccga ggaagtcgct
     2401 gttcagcgca agcgcgacag cgaggcggta gacgacggaa cccgcaacgc acgccgcagc
     2461 ccataccagc acggaccgcg tgccgaacaa cgcctcaccg acgatcacgg cggccaatcc
     2521 gaccacgatg gtgccggtgc caagcgtcac gtcggcgaat ccgttcattt gcgcgaagag
     2581 cgcgccggca agtgcgacga gtccattgct gagtgccatt ccgagatagg tggtgtgcct
     2641 cgtatcgaca ccctgcgcac gcgccatgcg cgggttgacg ccggtcgccc gcattgcaag
     2701 cccgtattcg gaggcgagga aacgggcgac caacaggatt gcggcgattg cgacgatacc
     2761 cgcaacgatc ggtttcacgt aaagcatcgc gagcccaagg ccatcgaagc tcgacaaaat
     2821 cgtcggctcc gagatgatgg cgacgttcgg ccggcccatg atgcgcagat tgatcgagta
     2881 gagcgcgatc atcgtgagga tgctggcgag cagatgcagg atgccgaagc ggatgttcaa
     2941 aaatgcggtc accagcccag cgaagcatcc gccgatgatc gccgcacccg tggctgtata
     3001 agggtcccag ccgctcacga tgagcgacgc gctgagggcc gcgccgagcg gaaagctgcc
     3061 gtcgacggtc aaatcgggaa agtcgaggac ccggaacgac agaaacaccc cgagcccgac
     3121 gaggccgaag acaagaccga tctcgattgc gcccaggaat gcgaccagat tcatggcgtc
     3181 attgacctct gggcgctgat gaccattcgc ggcggcacgg tgcgcgaagg cctacttcac
     3241 cacattcttt gcggtggcca acacggcctc cggaatggtc acgcccattt tctcggccgc
     3301 gcttacgttg aggtgaagct cggttatctc gaccccttcg accggaatat cgcccggctt
     3361 ctcgcccttg agcacgcgca ggaccatttt acccgtctgc ctgcccacgt cgtaatagtt
     3421 gaacccgagt gcggccattg caccacgcgg caccgaatcc gtgtcggcag cataaaccgg
     3481 gattttgttt tccgtaccga cgcggacgac ggcttcgagt gccgagacga tcgtgttgtc
     3541 ggttgggatg tatatcgcgt cggccttgcc gatcaggctc tgcgcggcag acagcacatc
     3601 ggaagagcgc gtcgcgggtg cctccacgat ctgcagcccg agcggtccag cttcctgctt
     3661 cagaagcttc agcagcgaca gcgagttcgc ctcgccggga ttatgcggaa cgccgatgcg
     3721 cttggcattg ggcgtaatct gctggatcag ctcgagatgc ttgcggatcg gcgacaggtc
     3781 cgagacgccg ctgacattgc cgccgggctt ttttgcatcc gtcaccagct tcgcgccgac
     3841 cggatcggtg accgccgtga atatgacggg aatgtccttc gtcgccgcca ccaccgcctg
     3901 agccgacggc gtggagatcg gcacgatcac gttcggccct tcgccgacca gcttgcgggc
     3961 gatctgtgcg gcggtggtcg gattgccctg ggcgctctcg tactcgaagc ggagattttt
     4021 tcccggctcg tagccgttgg ccttgagctc gtcgcgcacc ccgtcccggg ccgcatcgag
     4081 agccggatgc tccacgatgg cggtcacagc gacgaagacc ttctcctggg ccgcagcaga
     4141 aacgacaaga ccggtcgatc caaatgccag cgcggcgcag cccatgatgg ccctgagcga
     4201 ggaaaccatg cgaccaacct cccgtgcgag gaaagggtta agatcctgcg cgaaaatgcc
     4261 gggcaggttg tcggccgtca agctgagcac gcgccatcgg cgccaagctg cacttacaga
     4321 ttgatcgaaa ggccgaacgt agaggcaaga aggccgccga tgatgcccgc gagcggcggc
     4381 acgaaccagg agcacgcaag cctcagcccg acgaacggcc agcccatcat cgggctttca
     4441 taggaaatca cgcggtgaaa cgcgtatacc gaccatcccg tgatcagcgc gatcacctgc
     4501 gggccacctg ccccttggtg agcgaacagg atggcgatcg ggaacgagac gatcggcccg
     4561 cccggcatga acccgccgag cacagatgca gcggcgatgc cgagcaaccc ggaatccgcc
     4621 ccgagcacgg ccgcaatcgt ctgctccggc accagctcgg caatgcatga ggcggccacc
     4681 agcgcaaatg gcagccgcac gaaaatgcgc acgatttcct gacgcgcgaa catcgacgcc
     4741 cgcgccatga tgaactggtc cttgcgccag gcgtagatcg cgaccgggac cgttacagcc
     4801 agaaggatga ggaacgaccc gagcatcaac gatctccgcc cgaagcacgc attcgcccga
     4861 tcggcagatc gggtatgaag tgcatcaaga ggcgggcgaa gaggcccgcc acgaccggca
     4921 gcggcaggca cgccagcatg cgcgccagaa cgaagtcggg gccgagcagc ggcagctccc
     4981 agatgacgag gcgatgcagc ccaagcaccg accaagccgt caaataggtg accacggcgc
     5041 cgatgtctgc accggcgagc gccagcgcat agacgatcgg gaaggcggcg aaaggccctc
     5101 cgggcgtcgc tgcacccagt gcggttgcca gcagcagccc acgccagccg gatcgaggcc
     5161 caagcaccgg cgcgatgcgg tccgggtccg agagctcctt caccaatcca cccagaaaca
     5221 gcccgatgac aatcatcggc gtgacggtga tcagcagcgt caacgactgc gtaccggctc
     5281 tggtgagggc gtctttgcct tccagcagca aaaccagcag cgccgaagcg ccggcgagca
     5341 gcacgaagaa ccacgccgcc ctgtcaaaca caggcggcgg cgacttcccg gcgccggatt
     5401 cgtcttgcga gtggggtgcg gggtgcggag cgtcgttcat gggtctcgct gtccgtgccc
     5461 tctttggcag atcgcgcgtc ccatgtcacg ggtcgtgacc gcggccccga gatagattgc
     5521 ctacggcggc ccagccgcgg cgggagcagg tcgttcaggc tcatgatcac cgtcaactcc
     5581 aaggtcgatg gtgctcgcgt tccgtagctg ggcctatggc cgaatttggc ccaatccgac
     5641 ccgcccggct gttctctggg gaacagggcc gaacctctat caagttgggc cagaacacga
     5701 tcggctggca tctgccaatc agtttgctta ggattgcgca ggcaaactgc aggaggaccc
     5761 atgcgtgtgt tcctcagcct gtttctgccc gctgctacgg cgattttttc cctgctggcg
     5821 ctgatttcgc cggctcacgc cgtcgctccg gaccaatgcc ttgcgctcgc cgacaccaat
     5881 ccgcgcccgg tcatcgaacg tgtttcccta agactcgcgc agctgaaggc ggccgaggtg
     5941 cgcgtgacct acatcggtca ctcgactttc ctgatcgaga gcgcgggcgg cgtgagaatc
     6001 gccaccgatt acaatgacta tgtccgcccg gccgtggtgc ctgacatcgc tacgatgaac
     6061 cgggcgcaca atacgcatta cactcccagc cccgatccac gtatccccca cgtcctgcgt
     6121 ggttggagcg agggcggggc cgccatccac gatctcagcc tgctcgacgt ccgcgtccgc
     6181 aacgtgccga ccaacatccg cacctgggac ggcgggacca tggcttacgg caactcgatc
     6241 ttcgtgttcg agatcggcgg cctgtgcatc gcccatctcg ggcacttgca ccatacgctc
     6301 acgccccaac aactggccca aatcggccag atggacgtgg tgctcgtacc cgtggatggc
     6361 agctacacgc tagacctaga aggcatgctg gaggttctca aagcgctgaa ggcgccgctc
     6421 atgattccga tgcactactt ctcgggcgcc acgctcaacc gcttcctcgc ccgcgtcgag
     6481 gattccttcg agatcgtccg gcatgaggtg ccgacgatcg tcatttcgcg gcagacatta
     6541 cccaccacgc cgcaggtgct ggtgctgccg gggcgatgac ggtcatcgcc agctgttgat
     6601 caaggcttcc gcccagttct ggcggcgtcg gcggacgacg cgcacgcggc gacggcggcg
     6661 ccggcggctc gagcggtgcc ttacccgtgg ctccgcgctg acagcggaaa tcagaatgaa
     6721 gttgcgcggc gtggtgtgtt ttttcgaagt ggccggttca tccgttacga cgagaatcga
     6781 gccagcatgc aacgcccgac tcatcgtcag ggcgctggca cggtcctcga agctgatctg
     6841 atcgagcagc tcgtccccat gccaggagac gaggtgccgc tcgcgccgcg agcggccaat
     6901 cccgaacgcc agccagacca aaccttcgcc cgctggagcc gtaccggtga gcgagtagac
     6961 gtgggttccg atacggctgt tcggctgcac gaaccgcacc cgggccacgt gctccagctc
     7021 gccgtttcgc aggaggatgg cgctccggct ggcccgggag atcacgacgg cgacggggcc
     7081 ggcttccggc gtaccggtca gcaactggtg cccctggcgc tgagcgaccg gccgcaccat
     7141 ggggttcgcc gcctcataaa ccggcacgat cggaaacagc ccaccggaat ggacgacgtc
     7201 catgggcacg gttctctgcg gggccaggat caccgcggtg cccgggacaa ctatctcctg
     7261 gagcagccgt gcgaaggcgg cagggacgcg aatgcacccc ggtgacgccg gatagcgctg
     7321 cacaccgctt gcgtgcagcg ctatcccaga ccaggagcgc cggtcgtcat ccgcctcgct
     7381 gtcgtcgatc ccggagatcg cgaatacgcc gctcggggtg cggcgacggc gaaggccgac
     7441 atggcacgtc gaaatgccga ccagtacgcc cgccttgtag acatggacca ggcggtccgc
     7501 cagcgaagcg acgatggtga caggaccatc atgtggctgg gatggctccc agacgaactg
     7561 gcccctcttc agccctgttc gattgacgaa acgacgcgcc tcggcaggct gcacgagatg
     7621 tggcagaagc accgccccga ggcttatgct cagcatttcg cgtcgcgtaa gcgacatcgc
     7681 caactctcct ggtcgtgcga gctacggatc gggcgcaccg ttcctcacgc tcgtgaagag
     7741 tctatgcgat tcgcgatctg cgtgtaatgc gcagatgcga acgcgctttg gttgcatctg
     7801 acgcgagtta cacaacgccg ccgaccaaga ggccgattcg gactcgtctt agatcatgtt
     7861 gaaaagacag gcttaagcca agctagcttg tcagcgacgc ctgtggtgcg gacgaaagcc
     7921 tccgctaccc ggcggaggtg gcgttgcccg ggtatcctta tgccggaaat tgatgcgccc
     7981 cttcgtcaga tcatacgggg tcatctccac ggtcacgcga tccccggcaa ggatgcggat
     8041 ccgattcttc ttcatgcgcc ccgaggtgta ggcgatgacc tcatggccgt tgtccagcag
     8101 aacacggcac cgcgcatccg gcagaacctc ggaaacaaca ccttcaaact ctagaacctc
     8161 ttctttcgcc atatttctcc cttcagcgtc agacaccccg acgcattcga cccccggcac
     8221 ccgcagatgc cgggggcaag gctctttttc cgtcgcgtcg cttaggaaac gcgcaggttc
     8281 acagccgacg tcttgccgtt gcggccgcgc tcgagctcgt acgagaccct ctgaccttct
     8341 ctcaaggtcg acagtcctgc ccgctcaacg gccgagatgt gaacgaacac atcagaacca
     8401 ccttcctccg gctggataaa gccgtagccc ttctggccgt tgaaccactt caccgtacct
     8461 gtcgccatat tcgttctcgc aaccaatgcc gacctcaggg cccagtcccc gagaccatca
     8521 aataacgctt tggggacctt ggttatgcgg accttgcagc cgctggtggt atgcgctcca
     8581 agcgaattcg atgaagttat atgaacctac ggcgcgccaa acgcaagatg aaccgatatc
     8641 gtcgcatcga ccgattcgct ggccttcgga gtcggtgacg tgtggaggcc ataggtgaca
     8701 tgagggacga tgatggattt tcggaagctc gggctcggtt ttctcgccgg ctgcctggcc
     8761 gtcctgaccg cgcaccagct cactgtctac gggctctcgg cagcaggcct gatttcggct
     8821 caagcctggt cgaaggagcc ggcgggcctg ttcggcgtgc cggccgtcct caacagcacc
     8881 ttctggggag gagtctgggg atccatcttc gccctgagcg ccgatcggct gcctggcggg
     8941 agtttgtgga gcaaaggcct cgttttcggg ctcattttcc cgctcgtcat cggtgcctgg
     9001 ctcatcgttc cacttatcaa gggcttgccg tttttcgctg gtttctcggc acccaggctg
     9061 cttgccggtg cgctgatcca cgccgcttac ggcgcagcgc tcggcgcctt ctacggcttg
     9121 ctcacgcgcc gctgactgct gccgatcgtc ccacccggga ggcagctcgc cagcgccgac
     9181 cgccagatca acctgaccgt gcaacccact gaccgccgac cggaaccttc cacatttgag
     9241 cgagttgcag atgcggttcg cggccagtgg agagatgcct atgacagcca gacaagccgg
     9301 caaaatgacg gtcgaagaag ccggtcgaaa aggcggcgag gcgacgtcaa gcgtccatgg
     9361 tcgcgagttc tacgaggaga tcgggcaaaa gggaggagcc gcccgacgtg gccagctcgg
     9421 ccgcgaggga tattcggcgc tcggccgcaa aggcggcgag gcgaccagca acaagcacgg
     9481 ctccggattc tatgccgaaa tcggcaggaa gggcggcgag accgtcagcc gcaaccgcga
     9541 gcacatgagc cagatcgggc gtaagggagg cgaggcgcgc gctgagaagg gcgtcgagca
     9601 tcgcgctcgc gaacgccagt aagcagcgga gaaacagctc atggccaggg gcgtgggcgg
     9661 cgtctcaccc gccaacttgc agaagtacct ggcaggcatt tcctaccccg ccagcaagga
     9721 cgacctgatc tggaccgctc gccgcaatag cgcgccgaac gagatcatgg aggtcatcga
     9781 gaggcttccc ggagacaaat ttcgaggccc agaggaggtt cagagagcct atggcgacat
     9841 cacgtgacga cgcctgcgct gggcggaggt catcctgtgt aactgcgtaa cgagaggctg
     9901 ggtgtgtcat ggctaaaagc aggtcggacg gtggcaagat gaccgtggag gaggctggtc
     9961 gcaaaggcgg tgccgcgaca gcacaggcgc gcggtcggga gttctacaag gagattggcc
    10021 gcaagggcgg tgaggcccgc cgcggcaagc ttggctcgga gggctatcgt gagctcggcc
    10081 gcaagggtgg cgctgcgacc tctcagaagc gggcccccgt gcgggagtcc gagcatctcg
    10141 ctgggatcag ccagacccag caggcgatct cgaacgaaga gacgacgccg ggcctctcgg
    10201 agaccggtga catcgcgctc gaggaagagc accagatcga tcctgcagag cccgtgagag
    10261 gcacgtaagc aggcgcgttc tttccgcctc agagagcgat ccggagcggt accgggcggc
    10321 tttccccgac cggtgcagct cttcgtagga ccagcgagcc gcgcggcaat tgcccccgca
    10381 tcgcccagcg agccgtgcgc gcgttgctcg catcaacatg tcgcgtgctg ggctctcact
    10441 ttcctgtggc gcatgcgcgt tagttgagtg cgcgcgtcga ccggaaagcc caaggggcga
    10501 tcgattttgc gggtcgatcg gctcttggag caggtcaagg cacacctcaa ccaaccatgg
    10561 gttcagcttg atggcgccgg ccgatgcggc gcatgcccgc tgactgcgag cgccaatggg
    10621 ccatgtggga agaaatcatc ttctacatta ttgtgggctt tgcggcccag gtcatcgacg
    10681 gtgccctcgg catggcctac ggcgtgacgg tctcgaccgt gctgctgagc acaggcgtac
    10741 caccagcggt caccagcgcc tgcgtgcatg ccgccgaggt gttcacgacc ggaacgtcgg
    10801 gctttgcgca ctggtgtgcc ggcaatgccg atcgccggct gatcctgaga ctcgtgctgc
    10861 ccggaatgat tggcggcgca atcggtgcat atgtcctgtc gaacgtgccg ggcgagatga
    10921 tccgcccctg ggtcagcggc tatctgctcg tgcttggctt catcattctg ggcaaagcgg
    10981 tccgctaccg cccgtatcac cccgatccgc ccaaagccgt tgcgccgctc gggttcatcg
    11041 gcggcatgct tgatgcaatg ggcggcggcg gctggggtcc gctcgtcacc tctacgctcc
    11101 ttggctacgg ctctgcccca cggaccgtca tcggctccgt caatatgacc gagttctttg
    11161 tgacctcgac gatctccatt actttctttg ccaccattgg cctctcgctc tggccgatca
    11221 tcgcagggct cgtgctcggc ggcgttctgg ccgctccgct ggcggcactg gtcgttaggt
    11281 atctgcccgc gagggtgctg atggtgatcg taggttgcgt cgtcgtgggc ttgagcagct
    11341 ggacgatcct gagagtgatg atgacgtgac ctatgcagga tcgcgtttga gctctgtcgc
    11401 cgtgagccag taattcacaa gctggggcgc gctcgctgcg taggttcaca ataccgtttg
    11461 gctctcactt gtggcactag ggtccgcgtg acccctgctt tttccagccc ttgcggccgc
    11521 gcaacgctca cgaaaggctt cggatgtcgg gtccacaagg ccgaagcggt cggctcggta
    11581 gaccgtgatg tcgatcgagg gctccgtacc actaatcaaa tcggcaatga tctctccgaa
    11641 accaccggaa gctgccacgc ccgagccaca gcatccgccc gccaataaga agcgctcgta
    11701 tccaggaatc ggtcctagta cgaacttgcc atcaggcgtg taatttgaaa gacctgcaat
    11761 atgatgcgcg aaacgccatt cgtctatttt ggggatgacg gtccgtaagg cattcgcccc
    11821 ctcaaccaag agatcgaggt cgcgttcttc gtttgtcagc aacatgtcat tcatgtccgc
    11881 cgagagctca agcggattga aggtcaagga tttcggctct tgcagcccaa tgagtaatcc
    11941 gccgacttcg gagcgtagat aggcacgcat atccggcagg tggacattgg gttgtcctgg
    12001 agcgccgctg ccatcgggca ctgtaatcca gtaatggctg cgcgtcggtg cagcggcaaa
    12061 gccccagccc agccatgatg cgacctcggc accccaaacg ccggcggcgt cgaccaccca
    12121 gtccgcccgg ataattccct ggtcggtttg gacgcccgtc acacgcccgc tattcgccag
    12181 aaggcttttc gcggtgatcc cacgccgaac gcgcgcgcct aggcggcgag cggcggctga
    12241 atacgccgtg ccgagacggg caccatcgat gtatccgtcc gctggaatga agatgatccg
    12301 gcgcgcggca tcgagctcga gccatgggca cagaccatgt gccgccgcgg catcaactgc
    12361 ttcgaagtcg acgccggctg cggccaggca ctcctccatt gtctgcaact cattccagcg
    12421 cgaatcagaa aggatcgcgc gcaggctgcc gacttggcgg aagtcgattt tttcttcgag
    12481 cagggtttcg agctcgctga ttgcagcgac ggtgcgcttt accatgtgta tggtcgaggt
    12541 atccgagcgc gcatgacaaa cgagaccagc tgcgcgggcg gtcgcgccgc ttgcgagttg
    12601 attgcgttcg ataatgagca cgtcggcgat gccgcgcttg gccaagtggt acgctgcaga
    12661 gcagccgatc acgccaccac caatgatgac aacggcagcc tgatcgataa cagccggcag
    12721 tccactcatg agttatctcc tgattttctc agaaccgatc gggcctgtat ggcgaaacgt
    12781 cgatgaatgg tttgcggtca gccatcaggt cggcaatcag ccggcccgtt gtcggcgcgc
    12841 tggtcatgcc attgtggcca tggccgaacg caagcagaat gcgcctgtct gtcgggagcg
    12901 gaccaatcac gggaagcgag tccggcgtgc atggccggcg tcccatccac tcacttgcat
    12961 tggccagccg cagccccggc agccattcca ttgccatcgt gcccagttgt cgggcgcgtc
    13021 gaaagtcggg cttggccgaa ggccgcgcaa actcgaccgt gcctgcaaga cggaggccat
    13081 gctccatggg tgtggcgtag aacttggcat ccgcgaaaga gaccggcaag ggtagctcga
    13141 ccccgggctc cggcagatgc aagtgatatc cgcgttgcgt ttccaacggg atagagattc
    13201 caagcccctc gagaagcggt ttcgacgagg ctccggctgc aatgacgact cgatccgcct
    13261 caagtcgacg tccatcctcc agccagattg taacagcctc attctgcggc tcaacgcgag
    13321 cagcacgacc tatcaagagc tccccgccac acgcggtaaa atgcaccgcg agccgctgca
    13381 ccaggctttg cggatcggca acatagccgt gttgcggctg aaaaacgccg cattcgaact
    13441 tatcggcaag cgccggagcg atatcgtgaa tagccccctg atcgaggatt tcgaaatcca
    13501 ccccgcgttc ctgccgaagc ttccagccgc tcatagcaga ctcgagtccg cggcggtggc
    13561 ggtagacggt gaggctgcct gtccggcgga tcaggtattc gatcccggcg gcctgcaaaa
    13621 gtggcatgta actatcgaaa accgggctgt ggagcgcgtg cagggcatcg acaatctgcg
    13681 ggatgcgagt agcacgccct gcggcaataa aacggaacag ccaaggcatc acctgcagaa
    13741 aatatgatgg ccggacgacc agtggcccgt tgcggtcgat gagccatccg ggtacgtcct
    13801 tcagcaagcc gggcattgac aagggaatac agctcccagg gctcagcgca ccggcattgc
    13861 cgcgcgaggc accggctgcc ggttcttctg cctccagcag tgacacggaa tggccctcgc
    13921 gacggaggtg aaccgcgcaa catacaccca caattcccgc gccgaccacc atgatgcgtg
    13981 gcgccacact cttggtctga gacatgccga ttgagacgtc cggttgctac cctaccgcgc
    14041 tttccacgcc tgggctcggc gcgcgagacc tcgtgtggcc agcagatgaa cacaccgcgc
    14101 agcgatgcac gtcgctacaa tcaccatcgc catcgcagct gccgccgcat actcgccggc
    14161 gtcatccatg tttatcacag caatggaagc gagtgccgtc cggtgcgaat aaaggaacac
    14221 cgcagcagat acggtcgtca tgccgctgag gaacaggtaa atccagatgt caagaatggc
    14281 cggcaggcac aacggcagag tcacgacaaa aaaggtacgc aggcgcgaga ccttgagcga
    14341 gtcggacacg agctcgaact cgttatcgag ctggcgcagc gccgtaatcg cagttaagtg
    14401 gctgacggtg aagtagtgga tgatagtgga gatcaccaga accgccatgg tgccataaag
    14461 cacctcgagc ggattggttg gatcgttgag aaaaaagaca taggatagac ccagcaccaa
    14521 ccccggtatc gccagcggaa agacgcccag catgtgatag gcactccgca accaggctgg
    14581 cgcaggagac ttctcgctga ggtacgcgcc tacaaatatc accgcggtgc cgatcgtcgc
    14641 cgtcagaagc gccaatttca ggctgttgta gtagctctcc caaccgccac ccgcaaaacg
    14701 gtggaattca tagtttcgaa gcgtcagttc gagattgtag ggccagaact ttatgaacga
    14761 tgcatagcct ggcacaaaaa tcatggacag cattatgaca gtgatggcca ggcacagcac
    14821 cagggccatt cggtcccaaa ggaggctcgg tgatgggacg aaaggcaccg ccctgctcgt
    14881 gagcgtagca gcctgacgcc gccgcacgcg ccggtcgatg atgaatgcga acgcggccgg
    14941 gatcagcatg aggatgctga taaccgcgcc aatttggaag ttctgctgtc cgacgacctg
    15001 cttatagatc tcggttgcaa gcatgctgta ttggccgccg atcaccttgg gaatgccaaa
    15061 atcggtgacg cacaacgtga acgacgcaat gccagcgctc accaggccgt acttcatcga
    15121 aggtagggtc accgtcagga agatgcgcaa cggcctggcc cgcagtgcac gcgcagcctc
    15181 gaataggcgc gcatccgata gcgcgatagc agcactcagg ataatggaca catgcgggaa
    15241 gtgagcgact gacattgcaa tgacgatgcc aatcggccca tagatggact ggcccatcat
    15301 caagctgttg agatatccct ggctgccgaa cagctgagtg agtgccattg ccgaaagcag
    15361 cgaaggcgtc agcaggggca acagggcgac gctacggaag aaatctttga aaggcacgca
    15421 ggtgtgcgcc agggccagcg cgaacagcag ggcaaccccc accgttatga tcgtgctgac
    15481 gattgcgacg aaaagtgtat tccagagaac ctcggccgcc accggctgtg agaagaacgc
    15541 gacgtagttg gctaatccga taaaggtgcc gtcggcattc tgcaggctac gccagaaaag
    15601 cagtatcagc ggtgccactg caaacaggat caggaatagt gccagcccag tgagaatcag
    15661 ggtcggcgag aatagtaggc gaaacggcac gccgcgtgca tgcgatcggc tgactcgtcc
    15721 gatgtatgtt tcacttgtcg cggccacgcc gttccgcttc ctcatcgcgt cttcaagaga
    15781 aggccagcgt cacttcttct tgggctcggc tttgcccccg tagcgctttt cccactcggc
    15841 cagaatgcgc tcgcggtttt ctgccgacca ggcaaagttc tgcttcatga tcctctcccc
    15901 gatgtctttg ggcacgtaag gaatctcttt agccagtccg ggcatggcga caaccgatcg
    15961 tgttgctcca taaagtttca tggcttcttc agaagcggcc cagtcggcaa gtttcttggc
    16021 cgcatcgagc tttttcgtgc cgttgacgat cgccatggcg ttcatgtccc aacccagagc
    16081 ctcttccgga aggatcagct cgattggcgc gccatcttgc ttcagtcttg atcccctgag
    16141 ctcgaaggcg agtccgatca cctgctcacc acgagcagca attaccgccg gcgcgcttcc
    16201 agagtgggtg tagcggctga tgttctcgtg caggccgtcc atcagcttcc aggcctcttc
    16261 ctcgccgtac gtctggatcc aggcggtgac atacatgtac ccggtgcccg aggacgccgg
    16321 atgtggcatg acgatctgtc ccttgtagac ggggtctgtg aggtcgctcc aggtcttggg
    16381 agcaggaatg ccccgcttct tgccctcgat ctcgttgtaa atgattgcgc agatccatgc
    16441 cgagttccca aaccaatacg gcggatcggc cggatcgcgg aatttctcgg ggattttctc
    16501 gaagccggcc ggcttgtagg gcatcagaac gccccgtgcc ttcaggacat cgatgttggt
    16561 gaccgccagc ccccaaacag cgtcagcgcg agggttgtcc ttctcggcca gcaacttggc
    16621 ggcaacaacg ccggtcgagt cgcgaaccca gttgacgacg atgtcaggat gcgccgcttc
    16681 gaaggcctcc ttgtatgcag cgaggtcatc gttctcgtag gcggtataga cagtcagctc
    16741 ggttttcgcc tgtgctgcag ctgacgcaaa gccaagcccc agcgcaacaa ttgccgccgt
    16801 tgttagcgct ccaaaaagag gtctcattgc gttcctttcc ctttccaatg cttgttgagc
    16861 ttattttccc gttaaaagcg cgaattttcg acgaaggaat ggaaatgcga cttgcggccg
    16921 cgcacctgcc atcacgatcg gagcatgcaa agcgcgaccc gctgcttttg tccgccaggt
    16981 agctgtgccg gaacttctcc gtggcgtccg gcaggccaac cagctcgagg agcttgcgca
    17041 cgcagcgaag agcgctcgtc ccggctgagg cttccttctg ccgcaagctg tagccaacgt
    17101 ggcccgcaac ggtcttgctc gggaacgggg catcggactg gaatatgatg ccgaagtccc
    17161 gctgctccac cggcgggcgc gaggtatccc tggcttgcca gatctgcctc tcgcttgcgg
    17221 tatcaggccc agggcggcaa agctgccgta cctggtccaa agattccgca ccgacaggta
    17281 agaactcacc aattgagcgc cgtctctcaa agcctcaccc tggcgacacg tcggcaattc
    17341 gatctaatga tcgtgcatgg gttgctatct gtccaatctt tagtttcaat tgcgattatt
    17401 gatgaattag attgtgggcg gacgcttgcc tgaggggcac ggttcgagtg tccgacgcga
    17461 gggctcgaca atgaacttcc tgtatctgcg cgctttccat gcagtcgcca gcgagcgcag
    17521 cttcacgcga gctgcggagg tcctgcatgt gagtcaatcg acgctttcct ggcaggtgaa
    17581 ggcgcttgag gagcgctatg gtgtcagact actcgaccgt cgcggaaagc aggtcgtgcc
    17641 cacggaggtc ggcaaacgca cgcttcagct ttgcagggag gtgtttcggc tgcaggatga
    17701 aatcgaacaa acgctcaacg agacgcagaa gctgcagacc gggcggctca aggttggggc
    17761 ggacggcccg cgtcatgtcc tgcccgtact ggcccatttc atgcggctgc acccgaacgt
    17821 cagcatttcc ttgacgacgg gcagcgccaa gaaagtcatg agcgatctgc tcaactatga
    17881 aaccgacgta gctattgtag ctgccgagcg tccccgggag acgcagctcc atatggtcca
    17941 ctttcagact tacccactcg tcgcttatgt tccgcgcaag cacccgtggc gccggcgaaa
    18001 gtcagtcaag ccgattgatt tccacgggca gcaggtcatc atgcgcgagc cgacctcttt
    18061 gacgcgacac aagttcctgc gatcgcttca tagagctggc gccgtgcctt tcatggtcat
    18121 cgaaatcgac aaccgggagg ccacccgcga agcagttgcc ctaggtctgg ggatcggcgt
    18181 gatgggctcg tccgagttcc atggtgcaga tccgcgttgc gtttcgctgt cgattgaaga
    18241 tccagagctg cacttcaccg agtacgttgc ttgcttggaa aagcggcggg acatgcgcac
    18301 gatccgcgag ttcttcagaa tcgccgagat gttcgcaaca ccgccgccag cggcttcgaa
    18361 ttaattctcc tccatcttca gcgccgcgat gaacgcctcc tgcgggatct cgaccttgcc
    18421 gaattcgcgc atcttcttct tgccggcctt ctgcttctcg agcagcttgc gcttgcgggt
    18481 gacgtcgccg ccgtagcact tggcgagaac gtccttgcgc agggcgcgga tggtctcgcg
    18541 ggcgatgatg cggccgccga tggcggcctg gatcgggatc tggaacagat gcggcgggat
    18601 cagatccttc agtttctcgc acatcgcccg cccgcgttgc tcggcgcgtg agcgatggac
    18661 gatgatcgac aatgcatcga ccggctcgcc gttgaccaag atcgacatct tgacgaggtc
    18721 gttttcctcg tagccgatga tctgataatc gaaggacgcg tagccgcggc tcaccgattt
    18781 cagcctgtca tagaaatcga aaaccacctc gttgagcggc agctcgtaaa ccagcatggc
    18841 tcggttgcca gcgtaagtga ggttcttttg ccggccgcgg cggtcctggc aaagcttgag
    18901 aacggcgccg agatactcgt cgggcaccaa tatcgtggcc tggatccagg gctcctcgat
    18961 gtggtcgatc ttgaccgggt ccggcatgtc ggccgggttg tgcagttcga gccgggagcc
    19021 gtcggtcaag taaacgtggt agatgacgct cggggccgtg gtgatgagat ccatcccgaa
    19081 ctcgcgctcg agccgctcgg tgatgatctc gaggtgcagg agccccagga agccgcagcg
    19141 gaagccgaag ccgagggccg ccgaggtttc catctcatag gaaaagctcg cgtcattcag
    19201 cctgagcttg gccatggcct cgcgcagatc ctcgaagtcg gcggcatcga ccgggaagag
    19261 gccgcagaac accaccggct gcacgggctt gaagccgggc agcggctccg cggtcgggtt
    19321 tttctcatcc gtgatggtgt ctccgacgcg ggtatcggcc acctgcttga tggcggccgt
    19381 gaagaagccg atctcaccgg ggccgagctc ggtcagcatc tcctgcttcg gccggaacac
    19441 ccctacgcgc tcgatctgat aactcccgcc ggaggccatc agcttgatgc gctgcccctt
    19501 cctcagcacg ccgtccatga tgcgcacgag cacgacgacg ccaaggtagg cgtcatacca
    19561 gctgtcgacg agcagggctt tgagcggctt gttgcggtcg cccttcggcg gcggcaaccg
    19621 ctcgacgatg ccctccagca ccgcttcgac attgaggccg gtcttggcgg aaatcgggat
    19681 ggcgtcggac gcgtcgaggc cgatcacatc ctcgatctgc tgcttcacgc gctccgggtc
    19741 ggcggcgggc aggtccacct tgttcaggac cggcatgatc tcgaggtcgt tgtcgagggc
    19801 ctggtagaca ttcgccagcg tttgcgcctc gacgccctga gacgcgtcga caaccagaat
    19861 cgcgccttca catgccgcca ggcagcgcga gacctcgtaa gcgaagtcca cgtgccctgg
    19921 cgtgtccatc agattgagct gataggtctt tccatccttg tgcttgtaat cgaggcgcac
    19981 ggtctgggcc ttgatggtga tgccgcgctc gcgctcgatc tccatcgagt cgaggatctg
    20041 ctccttcatc tcccggtcgg tgacgttgcc gcaaagctgg atcagccggt cggacagcgt
    20101 cgacttgccg tgatcgatat gggcgatgat cgagaaattt cggatcaggg atgtgtcggt
    20161 catgccgggc tagatagcat cgggcccgtg gcggcaaaag gggcgcggcg ccctgtcaga
    20221 tgatagccgc acgcacccgc gccgtgatcc attcgctgag gacgacggtc gccagaatca
    20281 tgagcaggat cacggtcacc tgcggccacg cgaggacatt gagcgaggcc tcgagtgcta
    20341 gcccgagccc tcccgcgccg accagcccca gcaccgtcga ctcgcggatg ttgatgtccc
    20401 agcggaacac gcagatggtg gcgaaggccg gcatgatctg cggcaccatc ccataagcca
    20461 ttacctgggc cgggctggcc cccgtggcgc ggacagcctc cacctgagtc tcatcgatct
    20521 cctcgatggc ttcgtagagc agcttgccga tgaagccgat cgagcgcagc gcgatggcga
    20581 gaatgccggc gaaaacgccc ggcccgaata ccgccaccag cagcagcccc cagatgagcg
    20641 aattgatcga ccgcgaagaa acgatgatga gaagcgcgat cgggcgcacg aagaggatgc
    20701 tgggcgtggt gttgcgggcg gcgagaaagc ccagcggcgt cccgagcagg atcgccaacg
    20761 cggtaccgag ggtggcgatg ttgagcgtgt cccacagcgg cagccagagc ttgttggcgt
    20821 attcccagcg tggggggagc atgcgcccga agatgtcggc tgcctgacgc ggcgcgtcct
    20881 cgacgaacgc ccagatggtg ttggccgtca tcacccgcca gcagaatacc gtgagcgcaa
    20941 cgagtgccag ccagccgagc cagacggcga gttgccgcag ccgggttctg taccgccagg
    21001 tgacgggctg acctgcggct gtcgcgacga ccggcatgct tactgcgtcc acctgcggat
    21061 gaagctggag aaatactcac acgccatgac gatggcgatg atgagcatga ggatggcggc
    21121 tgcgctgtcg aactcgtaac ggtcgagggc tgtgttcaga gtggcgccga tgccgccggc
    21181 gccgacgatc ccgaccacgg ccgactcgcg gaagtttatg tcgacgcggt atagagaaag
    21241 gccgatgagc cgcggcttca cctggctcga cacggcatag tcgatcacct gccagaagct
    21301 cgcccccgtt gcccggatcg cctcgacctg gacgggatcg atctcctcga tgtcgtcggc
    21361 gagaagcttc gccaggaagc caatggtcgc gacggaaagg gtcaagaagc ctgccagcgg
    21421 accgaacccg aacatcgcaa cgaaaaagat ggcgatgatg atctcctgga aggtgcgcga
    21481 gagggccacg attccccggc agatgccgta gagccaccgg ggcgcgatgt tgcgagcggc
    21541 accaagcgcg atcggcacgg aaagcagaat gccggctatc gtggaggtga ccgtcatcgt
    21601 caggctttcg atcatgccgg cctggatgtc tccccagcgc gagacgaaat cgggcgtcag
    21661 aaaccccgcg aaaagccggc ctgcccgcgc aacgccatcg accactcgag cccagttcac
    21721 ctcgatggaa gaaaaggcca ggaccagata gagcagaacg ccgaggacgg cggcccagcg
    21781 gacccacgcc gtctcgatcg tccggggcgg gtgccatgtt cggggcaggc ccgctgatgg
    21841 aagggtctcg gcgtccatgt cagcgcatcg ccgcagtcag atcgcgatcg tgggcatgat
    21901 ggtccaaggc gtcgcgatac cgcccctcgg ccgcctcgcc gtcgccttcg gcagcagccg
    21961 acgtcttcga ccagtcctcc tcgccgtaga tgagggtcag cacctctggc gtgaggccgc
    22021 tcgccgggcc atcgtagacg atctcgcccg cccgcagtcc aacaatcctg ggaaggaaca
    22081 tctgcgccag agcgacgtcg tggatattga cgatggccgc caatcgccgc tcggcgcaaa
    22141 gctcggtgat gagccgcatg acctgacgcg acgtcttcgg gtccaggctc gccgtcggct
    22201 cgtccacgag cagaaggtcc ggctcctgca ccagcgcgcg agcgatgccg acccgctgcc
    22261 tctggccgcc ggagagcgca tccgcccgct tgtccacatg gtcgagcagg ccgacgcgct
    22321 ccagcacggc gaaggcccgt tcgacgtcag cctgcgggaa gcggcgaaag tagctcgtcc
    22381 aaaagccggt atagccgagc cgcccggaga gcacgttctc catcaccgtc agccgctcga
    22441 cgagcgcgta ctcctggaag atcatgccga tgcgacggcg ggcgcggcgc agcgcggact
    22501 tgccgagcgc ggtaagctcc tcctcactca ggaacacctt cccggaggtg ggctcgacca
    22561 gtcggttgac gcaacggatc aaggtggact ttccggcccc cgacggtccg atcaacccga
    22621 cgatctcacc ggagcctacc gagaaggaga cgccgcgcaa ggcggcatct cccgtcctgt
    22681 atcgcttcgt aagcgcttca atgcgcagca ttctcgattt ccgatcaagt tcggcgcagg
    22741 cgcttacttg caagtgtact tgacgcccat ggcggagtcg atcttgcgga ccacttccca
    22801 gtccttcttg taggtgatct cgatgaactt ggcctcgccg gatctcgaga attccttctg
    22861 cagctccgaa ccttcccacg ggaaggtgaa gaaggcttcc ttgatcttct cctgaagctc
    22921 aggcttcaga ttgtagacga ccccatagcc ggtggtcggg aaagtctgcg acttgtagat
    22981 cgatttgatc tgctcagcct tcaccacacc gcgctcgatc atgcgctgca tgacggaatt
    23041 cgcaatggcc gctgccggat aatccttatt ggcgacgccg agaaccgagt tgtcgtgctt
    23101 accggagaac actgcctcgt aatctcggcc agcctccatg ccgaactcgc tcttgagaag
    23161 cgccgaaggc gccttgtagc cggagttcga tgtctcggcg gtaaaggcca acttcttgcc
    23221 cctgatgtcc tcgaccttct cgatgccgga gcccggatag gtgatgatct ccatctcgta
    23281 tccgaacgag ttgtcggcgc tcgccataat ggcgaacggg cggaagccgg cgcaggccac
    23341 cgcgagcggg ttcgacccgg tattgaagcc tgcaatatgc agccgaccgg cgcgcatcgc
    23401 ctcgatctgg gcagcgttgg actgcaccgg gaaaaactgc accttcttgc cggtcacttt
    23461 ctccatgtgc ttgaggaagc catcccacac cttggcgtag acggccggat cctcgacggg
    23521 ggtgtaggca aagatcagcg tgggaggatc gatgagctgg ctaggatcag tgggagtgtc
    23581 ggctacgaga tcaccgtcat tgtcctggaa gcggggatcg agggcagaag ccggagccga
    23641 aaggatggca gcggcgatcg tcaaaaccgc aaggaaacca aagctccggt tgagaaaagc
    23701 cttcatcttg gggcacctcc ttaagaggac aaggttgaat ccgagcctcg ttcgattgtc
    23761 ctcttttacc gatggacgac agcggaggaa agctgtgatg tccctttggc ccgatccagt
    23821 ggccaatctg tcatgaccgc ggcttgggac gggtggtcgg cgcagcacgg atgggatcat
    23881 ccggccaggg atgcttcgga tagcgaccct tcatctcggc tttaaccgcc gcccaggagc
    23941 cccgccaaaa gccaggcaaa tcgcgcgtga tctgaattgg ccggtgcgcg ggagagagca
    24001 gatgcagcgt caatggcagc cgcccgcccg caatcgacgg atgccggtcg aggccgaaca
    24061 gctcctgcac cctgatgtgc agggaggggg catcggggcc actgtaatca atagggtggc
    24121 tgtggccggc cggcgacacg aaatgtgtcg gcgcctcggc atccagccga cgacggagct
    24181 cccacggcaa aagcccatcg agcgcggcgc cgaggtcgtc ggaggtgatc tcgtcaagac
    24241 gagtcttgcc ctggatgaaa ggtgccagcc attcccgcgc tttcgccgcc agggcggaat
    24301 cggagagatc aggccagttc tcgcctgtca tcgtccgcag aaactgaacc cggtcgcgga
    24361 gctgcttctg cgccttgctc cagggcaatc gatcgatacc gatttcggcg agccccgccg
    24421 ccagcttctc ggccgccgct tccggcggca cggggcgcgg ctcactcgcg agcacgatgg
    24481 cccccagacg ccggacacgg cgggcccgga gcgcgcccgc gtcgcgatcg aacgtgatct
    24541 cgtccgtttc aacgatccgg tcgcgggcta ttgcatggac ttcggcttcg tccgtcgccg
    24601 ccgcgagcag aatgcggctc gaagcggccg ctccttgcag ctcggccacg accagccact
    24661 ccgagcgcgc gagcggatcg gtcggatcga gcagagcgcc ccgaccgttg gcaagcagat
    24721 actgccccgg tgcgccacgg gccttcgcga tccggtcggg ataggccaga gccaaaagag
    24781 cagcgaccga agcaccctca gatccagctg tgagcgactg gcccgccgcc tctcgcatcg
    24841 cagcgcgcgc ccagccgcgc gctagatcgc gcatgtcgcg ggcccgccgc aagcggtcgg
    24901 ctcggaaaat tgcgaggcga tgctccagat ccgtatcgct cccgccgagg ccgcgctcga
    24961 ccagcagcgc agccatctcg gcggcttgct ccgcagcacc gaaccccgct gcagacagca
    25021 ccatccgcgc cagccgcggc ggcagcggca gcgcgcgcag gcgacgcccc aggccggtta
    25081 tccgcccctc ggcatcgagc gcgccaagcg cctcgagctc cgctcgcgca gccgacagag
    25141 gtgcttccgc gggtgggtcg agccattgca agcggcgcgg atcagtgacg ccccaggcag
    25201 cgcaatcgag cagcagtccg gacagatcag cgttacggat ctctggctcc gtgaacaaag
    25261 gaaggctggc cgttgccggc tcgtcccaca gccggtagca gacaccgggt tcggtccggc
    25321 ctgcccgccc acggcgctgg tccacggagg cctgcgacgc gcgtaccgtc accagccgtg
    25381 tgatgccgac gcccggctcg tagcgtggca ccctggcaag accactgtcg atgacgatcc
    25441 tgacgccttc gatcgtcagc gaggtctcgg cgatcgctgt cgccagcacc accttgcggc
    25501 gcccactttc cgcaggcgcg atcgcttcgt cctgggcgtg cttgtcgagc ccgccgtgca
    25561 gggcaaagat ctggaccgcg gggtcgtcca actgttcgcc cagccgctcg gcgacacggc
    25621 ggatctcgcg ctgacccggc aggaaggcga gcaccgatcc gcgctcgtcg cgcaacgctt
    25681 cgcggatcac ggccgccatc tgctcctcga tccgctgggc cgggtcgcgc ccgacatagc
    25741 gcgtctcgac cgggaaggcg cgcccttcgc tgtgaatcac gggcgcatcg ccgagcagct
    25801 cggcaacccg cgctccgtcg agcgttgccg acatgacgag cagccgcaga tcatcccgga
    25861 gcgcgctctt ggcgtcgagc accagcgcga gaccgagatc ggcgtccaac gaccgctcgt
    25921 gaatctcgtc gaacagcacg gccccgacct cctcgagcgc cggatcgtcg aggatcatgc
    25981 gggtgaacac cccctcggtg acgacctcga cgcgggtgcg cggtccgatg cgggagctta
    26041 gccgcgcgcg cagaccgatc gtttcgccga cggtttcccc gagcgtttgc gccatccgct
    26101 ccgcggccgc ccgcgccgcc agccgccgcg gctcgagcac gaggatcttg cggccgcgga
    26161 gccaatctgc acccatcaat gccagcggca cgcgcgtcgt cttacccgca ccgggcggcg
    26221 cgaccagcac ggccgccgtt ccgcggctga gcgcatccgc aagctcctgg agaacggcgt
    26281 cgatcgggag aggggcatcg aaacgcatgg ggcctgcatc agccgcaagc ctcggctgcc
    26341 gtcaacctct ctcggctcca tccagcgcgt tgatatcgag gctgacacga ccgaaacggc
    26401 atgctatttg gaattgttct aaagtggagt tgctcgcgca tctctccaag gaggccggat
    26461 tgaggacatt cgccgagctg agtgagaaag agatccttgc cctcgccatt tcgctcgagg
    26521 aggagcacgg acgcatctac gacgagtacg cccacgcgct ggcggacaac ttccctgcct
    26581 cggctaaagt cttcaaggag atggccgccg aggaggacga gcatcgccgt tggctcatcg
    26641 acctttatcg ccagaagttc ggcgaccaca tccccctcat ccgcagccag gacgtgaagg
    26701 gcttcgtcaa gcatcagccg atctggctcg tccggccatt ggggctcgac aaggtgcgcg
    26761 cccaggcaga ggcgatcgag tacgagacgc gccgcttcta cgaaaaggca ctggagcgcg
    26821 cctcggacgc gtccgtacgc aagctgctcg gcgacctcgc ggctgccgaa cgcaaccaca
    26881 tctcgctggc ccagcagctc ggcgaggccc acctgacggc cgacgccaga gcgcaggagg
    26941 cggaggccca gaggcgcatg ttcgtgctgc aggtcgtcca gcccggattg gccgggctga
    27001 tggacggctc cgtctcgacg cttgcgcctc tgtttgccgc agccttcgcc acccacaaca
    27061 cctgggagac attcctggtc ggcatggcgg cctcggtagg tgctggaatt tcgatgggct
    27121 ttgccgaagc gctttccgac gacggctcga tgacagggcg cggccggccg tggattcgcg
    27181 gcgtgattac cggcctcatg accacgctcg gcggcgtggg gcacacgctg ccttacctta
    27241 tcgcagactt cgggtccgcg acggcactcg ccatagcggt ggtgctcgtg gagctttggg
    27301 ccatcgcctg gatcagagcc cgctacatgg acacgaggtt tctccaggcg gcgttcgagg
    27361 ttgtcatcgg cggcctcatc gtgttcgcgg cgggcattct catcggcagt gcttagcccg
    27421 acagcgcgcc attatcgtct taacggtaag agcgggcggg gcctgatccc gcccaaattc
    27481 tacaataatg tctcgctatc tgcttttttc gttaattatc tttaacaaat tcccgtgatt
    27541 tctgctgctg attccgcaac tctcgcatca tggatgagaa ggcggaaggt tctcgtaaac
    27601 gtttgtcgga tcgtcagggc tcgactgcct ttgccactcg gctgaagcaa ctcagggttc
    27661 tgcgcggcta tcgcactgcg cgcgcttttg ctcaggccct gcaaatcgac gagaaccgct
    27721 atacgcgctg ggaacgcggc gaggtcgaac ccagcgtcgc gatgctggcc aagatggccg
    27781 aagtcctgaa cctccccgtt gatattctcg tcagcggagg agatgtcgct gtgggcttgc
    27841 cttcccccat ggcggtgcgg gattttgggc cgcgcgtcgc atcgagccac ggcaggcccg
    27901 aacatcccgg catgcaggag gaagccagcg gcttcacgtt cgagcgcacc ccggaggagc
    27961 tcagccagaa ggtcttggcc gcctcccgca catacctcat gtcgctgctc gagcggcggg
    28021 ttgccggcat gagccagaac gagatcgatt cgctgattgc aaagctcacg acggcgccgg
    28081 attccgatct gatccccttg gagaccaagg aaccgccgga taagccctcc agcagatagc
    28141 gcttggatag cgctggccgg gagctgtagc gtcccctgac gcttctcgaa aaatcgccgt
    28201 ctacggtcgg cagcagaccg gcaagcctaa ccctcgcccg cggaaccgca tcggttgggc
    28261 atgcgttcgg cgtgtgctaa acagcacatc cgcgtgaacc aattccagga gatcagccga
    28321 tgaagggtcc cgagatttcg ctcaagcatc ttcagcgggc catcacgatg gagctgacca
    28381 ccgtcaatca gtatcttctg caagcgcatc agctcgcgaa ctggggcatc gatcggttgt
    28441 cggaacgaat gttcggagag atcgaggagg agcgtgagca cgcgaacaaa tacatcgagc
    28501 gcatgctgtt cctggaaggc gagcccaacg tgcgggacct cgacgagatc gtaaagcctg
    28561 aaagcgtgcg gcagatcttc gaaactcagt acaagatgga gctcgacgcc cgcgcctatt
    28621 acgacaaagc agcacgcgag tgccaggatg caggcgacag cggcacgttc gagcttttcg
    28681 tctcaatcct gaaagacgag gaaagccaca tcaacttcct cgaggatcag ttcgatctga
    28741 tgaagctgat gggcgatcaa ctctatatcg cccggcaggt ctcgtccgtc gccaaggacg
    28801 aagacgacct ctgagcggct gagaggcgcc cccacgttac gggtcagcac tctcgtgtga
    28861 cggcagccga acgtccgcac ccccaaccaa atccccggcg aaccgcagcg gaggggtcac
    28921 cgcacccctc ctggagagcg acgtcctccc ggcgtgatcc cggggcaaac ctcatccggg
    28981 atgttagcag tcgataacca tcattcgctg atgacacaat tgcgcgcgaa agatatgcgg
    29041 attggcgatc gtgcaccaag aggctgtcgc tcatgcaatg acacgatctc ggccaacctc
    29101 gtccggaccc attcagccgc gcccctcgcg cggcgcggac gcgcgcaggg cgcaaaagcc
    29161 acgctcgacc accttgcaga cggcgctgct ctggttcgtc gtgagcgttg ctgcgctggt
    29221 cgtagccgcc acgacgttcc tcattatcgc cacgcctacg gatctgatcg aagaccggat
    29281 cgcgcaggag gtgcaccagc ggacaggtcg gaacctcgtt gtcgctggcg gaagctcgct
    29341 cagcatcctc gacgggctcg ggatcaagct gcacgacgtc tctctggcct ctccacccga
    29401 catgggcggt gggccgatgg ctcgtgtcgc cgaggtggac gtcaaaatcc cgttctggcc
    29461 gctgatccgt ggcgagctgc gggtcgatag actcgtgctg cggcagccgg agctgtcgct
    29521 ccgcgtcgat tcggacggcc gccggaattg gaagttcaat cgacctgtca ggaaagccgg
    29581 cccgcagcgg accggcgcgg gcggcggcac tgtcggaagc gctctcgccg gcacccggac
    29641 gcctctgccc cttgctaacg tgctgatcga agacggcatc atccgttatg cgaacgagaa
    29701 aagcgacgtg cgcgaggaga tcagcggcct tgatctccag gtcagcgcct catcgctgga
    29761 tagccggctt gaagcaacag gtgacctcac catccgcgac caggagttcg agtttcgagg
    29821 cagcctgagc tccctgtcgt cgttgctggc gggggcgccg tccgaatttg ccatcgatct
    29881 caaggggcag ccgctgaagg caaagtacga ggggacgatc ttgacatcgc ccgccacgca
    29941 gacagccgga accgtggccg tccaatcgag ctcgatacgg gccctcgcgg actggctcga
    30001 tagttccgcc ttcgacgagc acaaggacga gccgctcgtc gtatcaggcc gattggagct
    30061 gacgccggcg gcggtaacac tcagcgaggc cgatgtacgt ttgggcgata cgacagtcga
    30121 tggcttggcc atcctggaaa gccgctccgg cacccgccct cgcctcagtg ccgacctgca
    30181 cgtcagccag ctcgacctcg gctattggct gacaccgacg ggaaaggccg cgagctctag
    30241 agtcgatgga ggcggaaact ccggcatcgg cgatgaaagc gagccgcaga gtatcgacga
    30301 cctgctgcgg ggaacgcaga ccgatgggac acaggtgcgc ggcttcattg cccgtcacgg
    30361 ctggagcgat gttccgttcg attacgaaaa gctgggcgca atcgatgccg atttgaaact
    30421 gctggtcgac caggtcacgt accggaaagt caaggccggg acggcgcgca ttaccgcgag
    30481 gctggccgac cgccgattgg atgcaagcat caatgaaatg gagctctacg acggcacggg
    30541 gcgcggaacc gttcaactcg atgcgaccgg atccgtcccc accattcagg cagagctgca
    30601 gatcgataac gtctccgcgt tcgatttgct gagggatgcc gccgaattcg actgggtcga
    30661 cggcaagggc cggatctcga tcgcgatcac gggacaaggc caaaccgaac gggaagtcgt
    30721 cgaaaccctg aatggaaagg ccgagtttgc ctttcgcaac ggcgcgctcg tcggtgtgaa
    30781 tattccaggc ataatcgagt cccttcagcg gggccgtatt ccccggctcg agcgcaatat
    30841 ggcagagcgg acgaaattca gtgcgcttga cgccacgttc agcattgaaa acggaaccgc
    30901 gcaaaaccgc gatctgcgtc tggagagtga gctcatccac gtcagcgggg caggaaaagc
    30961 aaacctgccc gatcgaacgc tcgattatac cgtgcgcccg aagctcctga gcggtcgtaa
    31021 agccaatggg ggggagtctt cttccggcct cgagttgccg atccggatca cagggtcctg
    31081 ggatcacccg acctatgccg ccgaaatcga ctccgtgctg aagaatccgg atgatgtcgt
    31141 cgaagccttc aaggagatcg gaaagcagct caagggcaag aacttgagcg aagcgctgga
    31201 cagcctgctc ggtggaaatg atggcgatga cggccgcaag aaaccgaagg cccgcgagct
    31261 gctgcggcag tttctaaagc cgtgagggaa attgaggaca gctttgcgcg cgagccgaac
    31321 caagctgacc agtttggatt gctgtccaca caagcgggcc gcggttcagc cgcggcccgg
    31381 atttgtagtg cccagtcgag aaaccgaact attggaaggc ggacagcttg ccgaagtgct
    31441 ccgccagcag gtaatagacc gtcaccctgt ttttcgcccg ggcccccttc atcctctcgc
    31501 cgacggccga gatggccttg tcgagctcgg catcgtcgag agtgaggcct agcttcttct
    31561 tgaggaagtt ctcgcggaca cgcgccagct cttccttgtc gctgaacgag accagggatg
    31621 aatccctctt ctgcagagca attccgcaat agcggacgat gccgg
//
LOCUS       DASVDU010000185         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_98868, whole genome shotgun sequence.
ACCESSION   DASVDU010000185 DASVDU010000000
VERSION     DASVDU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98868"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VF226_12135"
     CDS             <1..748
                     /locus_tag="VF226_12135"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HEX7074783.1"
                     /translation="VNAIAPGRRSRTRDAVGAIRHVFLEADQDGPAVLAAIAARRDLP
                     PPSYVLHSSPNRVHVFWRVHGFDPACVEALQKRLANELGTDTAATPATQTTRLAGFRN
                     HKYAAPHLVTIEYLDTEAVHAPAAFPRVELRAVPHVRPLTPAERRAASRLDPVERARR
                     YLAKVPPAIAGQHGDLHTFRVCCRLVRGFALDDQNALAVLLEWNAGCVPPWSERELRD
                     KLQRAQRYGRETRSGAISHSHTPPGLPPRL"
     gene            1260..1742
                     /locus_tag="VF226_12140"
     CDS             1260..1742
                     /locus_tag="VF226_12140"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074784.1"
                     /translation="MVLSDAGQECRIWNAKPIDSQRWVPPPQPNAWPLGDTDTQEEFS
                     FYDALGRATAYLDFSDGLVFYLWTGEPVAYLDGDSIYGFNGKHLGWYHNGLVYDEDGN
                     VVCAPAAAFREPPNPGPIRSLKQLKPLKGLKELKPLKPLFGRAWSRLPARVFFLMGVN
                     "
     gene            1941..2474
                     /locus_tag="VF226_12145"
     CDS             1941..2474
                     /locus_tag="VF226_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074785.1"
                     /translation="MKNKSETLFEAYLRANGYFDFDYEKEIQGSAQRPDYTLRFNGAE
                     ILFEVKEFQATPGDFTPGFDYFDPYRPLREKIDAARQKFKRLKTYCCCLVLYDVNKPL
                     VLMDWQHIYGAMLGNVGFSLPLDIPGCPKSPNRSILSIFMGEGKMTRWLKGVIVGPQN
                     RTISAILVLAARGETLI"
     gene            2556..3908
                     /locus_tag="VF226_12150"
     CDS             2556..3908
                     /locus_tag="VF226_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7074786.1"
                     /translation="MWVATQDLPRSAAHPFYARLNQILDQHDFDGFVEGLCERFYAEE
                     GRPGLPPGRYFRLLLIGYFEGLDAERAIAWRAADSFALREFLGLVLPDGPPDHSTISR
                     TRRLIDLETHEAVFTWMLQRLADAGLVKGKTVGIDATTLEANAALRSIVRRDTGESYQ
                     DFLIRLAQASGIDTPTRADLARIDRTRKKKGSNDDWTHPHDPDAKITKMKDGRTHLAH
                     KAEHAVDLETGAIVGVTVQDADDGDTTTCVETLIEAAQQVETVRPDGEGIEEIVGDKG
                     YHSNQCLVDLEAVGVRSYISEPDRGRRNWKKNPKARDAVYRNRRRIRGPRGLRLLRLR
                     GERLERPFAHLYETGGLRRIYLRGHTNIRKRLLIHAGGFNLGLLMRQLIGVGTPRGLQ
                     GRVAAVLVALLTLIRTRWDAITSHRAPVRRFSRLEPLSIVCAPVAQIGERKLAFTTGC
                     "
BASE COUNT          837 a         1392 c         1404 g          865 t
ORIGIN      
        1 ggtgaacgcg atcgcgccag ggcggcgctc gcggacgcgc gatgccgtgg gcgcgatccg
       61 gcacgtattt ctcgaggcgg accaggacgg accggcggtg ctcgccgcga ttgccgcgcg
      121 gcgggacctg ccgccgccgt catacgtgct gcactcatcg ccgaaccgag tacacgtctt
      181 ctggcgggtt catgggttcg atccggcgtg cgtcgaggcg ttgcagaagc gactcgcgaa
      241 cgagctcggc accgacacgg ccgcgacccc ggcgacgcag acgacgcggc tggccgggtt
      301 caggaatcac aagtacgcgg cgccgcacct ggtgacgatc gagtacttgg ataccgaagc
      361 ggtgcatgcg ccagccgcct tcccccgtgt ggagctgagg gcggtcccgc acgtgcgtcc
      421 tctcacgccg gctgaacgcc gcgcggccag ccggctggac cccgtagagc gcgcccggcg
      481 ctacctggcg aaggtcccgc cggcaatcgc cgggcagcac ggcgacctgc acacgtttcg
      541 cgtgtgctgt cgactcgttc ggggctttgc gcttgacgat caaaatgcgc tcgcggtcct
      601 cctggaatgg aacgctggct gtgttccacc atggtccgag cgtgagctgc gcgacaaact
      661 gcagcgagcc cagaggtacg gacgcgagac gcgaagtggg gcgatatcgc actcccacac
      721 gccgccagga ctaccgccaa gactgtaggt cccgaaccat tcgaatgggt cggcgctctt
      781 gggggtagca tagtgcgcgt aagtcgttga taacctttga cttatgttga gctggccaag
      841 atcaggaaga acctcatcag tggtcgccct ggacccgagt catccaagtt gagtccaacg
      901 cgctgggcaa gctcatgcta tccttgagcc acctcagccg ttgttgcgct ggatcggagg
      961 cggtgcgtgt gcgtcccttt ttggggaacg ccgtaccggc gaatccaatg atgacacgac
     1021 gggtagttca agccgtcctt tgcgcgtgtg cgctcgtcct cctccaatcg cccactatcg
     1081 tagcgtcagc ggagtacgtt ctagttcaga gggttatcgg cgatcaggct gtgattatca
     1141 gatcaaacgg cgctatctac ctaatcgaga aaggcattgg atgcctctct ctatcgaggt
     1201 acgagggtcg aagagtaatc atcgattcgc caggactatt cctgggcgtc gggtccaggt
     1261 tggttctttc cgatgccggt caggagtgtc gaatctggaa cgccaagccg atcgattcac
     1321 agcgttgggt tccgccacct cagcccaacg catggccact tggcgatact gacactcaag
     1381 aggagttctc attctacgac gcactgggcc gagcaaccgc gtatcttgac ttctccgatg
     1441 gacttgtatt ctatctctgg acaggcgagc ccgtcgcgta tctggacggc gacagcatct
     1501 atggcttcaa tggcaagcac ctggggtggt accacaacgg cttggtctat gacgaagatg
     1561 ggaatgtcgt ttgcgcgcca gcggccgcct ttcgggaacc gccaaatcct gggcccatta
     1621 gatcgctaaa gcaactgaag ccgctcaagg gactcaagga attgaagcct ctcaagccgc
     1681 tgtttgggcg tgcatggtca cggttgccgg caagagtctt cttcttgatg ggtgtcaatt
     1741 gattgtgttt cgcgtatcag gttgtactgc aagggaactt gccgtcgaac tagtgttcag
     1801 gcgattacga aaagaatgtc tttcgaactg gtcgcctgct cccgaggctc ccttgttgcg
     1861 ggctccgcct cggacccgag ccacgacctg cgcattcagc atcttacatg ctcgacgagg
     1921 gtcggaggcg gagcctcgtc atgaagaaca agtcggagac tctcttcgaa gcgtatttgc
     1981 gcgccaacgg ctactttgat ttcgactacg agaaggagat tcagggttcc gcccagcggc
     2041 cggactatac ccttcggttc aacggcgccg agatcctgtt tgaggtcaag gaattccaag
     2101 caacacccgg cgatttcacg cccgggttcg actatttcga tccgtatcgg ccgctccgcg
     2161 aaaagattga cgcggcaagg cagaagttca agcgactaaa gacctactgc tgttgtctgg
     2221 ttctttacga tgtcaacaag cctctcgtgc taatggattg gcaacacatc tacggcgcga
     2281 tgcttgggaa cgtgggcttt tcattgcctc ttgacattcc gggatgcccg aaatcgccca
     2341 atcgctcgat tctgagcatc tttatgggcg aaggcaagat gaccaggtgg ttgaagggag
     2401 taatcgtcgg accacagaac cggaccatca gcgcgattct ggtgctagca gcccgtggtg
     2461 aaacgttgat ttagggatcg acacgatcga tcccgcgtac tacactgtcg ccgatggcga
     2521 tgggcaaacg aaagcggcac gcgaagcaag tgtcgatgtg ggttgccacg caggacctgc
     2581 cgcgcagtgc cgcccacccg ttctacgcgc gactgaatca gattctggac cagcacgact
     2641 tcgacggctt cgtggaaggg ttgtgcgagc ggttctacgc ggaggagggc cgaccgggcc
     2701 tgccgccggg ccgctacttt cgcttgctgc tgatcgggta tttcgaaggt ctggacgccg
     2761 agcgcgccat tgcgtggcgt gccgccgatt cctttgcgct gcgcgagttt ctcggcctgg
     2821 tgttgcccga cgggccgccg gatcactcga cgatttcgcg tacccggcgc ttgatcgatc
     2881 tggagacgca cgaagccgtc ttcacctgga tgctgcagcg cctcgccgac gcgggcctgg
     2941 tgaagggcaa gaccgtcggc atcgatgcca cgacgctcga agccaatgcg gcgctgcgca
     3001 gcatcgtgcg gcgcgatacg ggcgagagct atcaggactt cctgatcaga ctcgcgcagg
     3061 cctcgggcat cgacacgccc acgcgggccg acctcgcgcg gatcgatcgg acgcgcaaga
     3121 agaagggctc gaacgacgat tggacgcatc cccacgatcc cgacgccaag atcacgaaga
     3181 tgaaagacgg acgcacgcac ttggcgcaca aggccgagca tgcggtcgat ctcgaaaccg
     3241 gcgcgatcgt cggcgtcacg gtgcaagatg ccgacgacgg ggatacgacg acgtgcgtcg
     3301 agacactgat tgaggcggcg cagcaggtcg agaccgtccg gccggatggc gaggggatcg
     3361 aagagatcgt cggcgacaag ggctatcaca gcaatcagtg cctcgtggat ctcgaagccg
     3421 tgggcgtccg cagttatatc tccgagcccg atcgcggccg acggaactgg aaaaagaatc
     3481 ccaaggcccg tgacgcggtg taccgcaacc gtcgccggat tcgcggtccg cgcggcctgc
     3541 gcctgctgcg gcttcgcggc gagcgcctcg aacgcccctt cgcccatctg tatgagacag
     3601 gcggtcttcg tcgcatctat ctccggggcc acaccaacat tcgcaagcga ctgctgattc
     3661 acgccggcgg cttcaatctc ggcctgctca tgcggcagct catcggcgtc ggcacgcctc
     3721 gagggctgca gggacgcgtc gccgcggtgc tggtggcgct tttgacgctg attcggacgc
     3781 gctgggacgc gatcacgtct catcgggcgc cggtacggcg cttttcacga ctcgaacctc
     3841 tgtcgatcgt gtgcgcgccc gtcgcgcaga tcggcgagcg caaactggct ttcaccacgg
     3901 gctgctagct gcccgccaac gccactctgg cctcgccgct cgacgagtga ctcgacacgc
     3961 ttcgatcatc tacggcagga ctctctttct tggctggcca atgccctgtc ggccactacc
     4021 gccccgacat ccagatgtgt gggtcgatgg gtcgcgacgt ggcggctcga agtcaggatc
     4081 acggactgat agggcgaccg ctcgcaagag gttcgatccc gtccagacgg ccctcaggca
     4141 ccgcggcagc cgcgattctg tgtcgaagcc aggccgattc cggcgagcat tcttcggcgt
     4201 tacttttctg cccgccgcgg cgttcattta ggtacataga cggctccgcc cgtcgccacg
     4261 cgcccgcccc tcgggccggt gcgtcccacc tcgttccgcg catcctccgg attcgccgga
     4321 acttcactcg cgccgtcgat ccccgatccg cggcgtaccc caggaggacc tatgtccaag
     4381 aagaacgcag aggtcgtgtc tgtccggatc ctcccgaata ccacgggcaa tccccccggg
     4441 aagctcgcgg acgcggaggt gatcttcgaa gccgatgccg gcccgctcag cgggatga
//
LOCUS       DASVDU010000186         9130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_530408, whole genome shotgun sequence.
ACCESSION   DASVDU010000186 DASVDU010000000
VERSION     DASVDU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9130
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530408"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF226_12155"
     CDS             <1..425
                     /locus_tag="VF226_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074787.1"
                     /translation="GVLGVLYALSIGSFTLFLLSFLFLFFMSGVGNASTFQMIPVIMR
                     LEMDRLMPGATADERQRQAEKEAAAIIGFTSAIAAFGAFFIPSAYGVSLALTGGAQAA
                     LWGFLIYYISCIALTWGQYVRPGGLLFEIERRQQRQAA"
     gene            complement(465..1187)
                     /locus_tag="VF226_12160"
     CDS             complement(465..1187)
                     /locus_tag="VF226_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7074788.1"
                     /translation="MRASDAENIRRLPLFRDMDHASFEKLVAAGYLQRFPDRVDLVYE
                     GDRPDFLHVILEGAVEQFARHGDRETTLSIIRAPAAFILAAVVLDQPHLKSARTLTPS
                     LIALIPADAAREVFEEDPAFARAALRELAVRYRAIVKDLKNMRLRPSLERLASWILKA
                     HNEAGGRGIFTIPMEKRSLASQLGMRPENLSRNFAELARHGVKVNGRRVRIDDLTALT
                     ALARPNPLIDEPELYPSDNKQA"
     gene            1418..4162
                     /locus_tag="VF226_12165"
     CDS             1418..4162
                     /locus_tag="VF226_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074789.1"
                     /translation="MTAHPTALPGDQNRALWLSTAAFTACFAVWTIFSIIGVQIKSDL
                     DLNETQFGLLVGTPILMGSLIRLLLGVWTEQYGGRVVYTAVMLSAAVATWLLTFAYDY
                     PTFLLAALGVGIAGGSFAVGVAYVAKWYPEQKQGTALGVFGAGNVGAAVTKFVAPFVM
                     VAYGWQAVANIWAIALAIVGILFWVFAKDEPQLEARRKAGVKPEPMLSLIAPLKNLQV
                     WRFSLYYFFVFGAFVALALWLPRYLIGVYGLDIATAGMIGAFYSVPASIFRIYGGHLS
                     DKYGARRVMYWTFGVSVACTFLLSYPPTEYVVSSMRGPITFRLETGLFAFTVLIFVLG
                     FFMSLGKAAVYKHIPVYYPNHVGPVGGVVGLVGGLGGFVLPIAFGLLNDLTGLWQSCF
                     MLLFAIVTVALVWMHVSIRIMERGVLEPELKKLPELPEMQEIHEPRHAGVLGPHLIED
                     WRPEDEEFWKTKGRAIARRNLIISIPSLLLSFAVWMVWSVVVAKLPAVGFTYTTDQLF
                     WLAALPGLSGATLRIFYSFMVPIFGGRLWTTLATWSLLIPAVGIGLAVQNPNTPYWVL
                     LLLALTCGFGGGNFASSMANISFFFPKSEKGNALALNAGLGNLGVSVVQFLVPIAITA
                     GVFGWLGGDPVIVKEGGREVPLWLQNAGYIWVPFIVASAFAAWFGMNDLATARASFAE
                     QSVIFQRKQNWIMCWLYTGTFGSFIGFSAGFPLLAKTQFPNIDVLPYVFLGPLIGSLS
                     RVLSGWLADKYGGGRVTFWVFIAMSIGVLGVLYSLNAGSFVGFFAMFLFLFFATGVGN
                     ASTFQMIPAIMRKEMDRLMPEATVAERLRQAEKESAAIIGFTSAIAAYGAFFIPKGYG
                     TSIALTGGPQAALVVFLIYYVTCIALTWAVYTRPGGLLYDVERGGGIKSKTAAARAAP
                     "
     gene            4179..7916
                     /locus_tag="VF226_12170"
     CDS             4179..7916
                     /locus_tag="VF226_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX7074790.1"
                     /translation="MSHFLDRLTFFKRPQETFSGGHGITTREDRSWEDGYRKRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRPDLPNHEPRGCSRGASYSWY
                     LYSGNRVKYPLVRSRLMRLWREARKTLTPVAAWASVVEDAEKRASYVKKRGLGGFVRA
                     TWDEVTEIIGAANAYTVRKYGPDRIFGFSPIPAMSMVSYAAGSRYLSLLGGVCMSFYD
                     WYCDLPPSSPQTWGEQTDVPESADWYNSGFLILWGSNVPQTRTPDAHFYTEARYRGAK
                     SVVICPDYSEASKFADMWLSVKQGTDAALAMAMGHVILREFHVDRQVPYFEDYARRYT
                     DMPMLVRLVKQGDNLVPERMIRASDFDDAMGETNNPEWKTVAYDENSGEIVVPRGSIG
                     FRWGDKGKWNLEEKDAKGNPIKLRLSLAPIKDDIAAVAFPYFGNREHDHFTGTDHPSI
                     LPRNVPVKRLKLKDGEALVASVYDLFIANYAVDRGFGGRHMAKSYDAEEPYTPAWAEK
                     ITGVPRDKIIHVAREFARNAEKTNGRSMVIIGAGMNHWYNSDMNYRGVINMLVMCGCV
                     GQSGGGWSHYVGQEKLRPQSGWLPLAFALDWARPPRQQNSTSAFYAHTDQWRYETLDV
                     SDVLSPTAPAGPWDGALIDYNVRAERMGWLPSAPQLQANPLDIAKKAAAAGKEPKDYV
                     AAALKSGELKLSCEDPDNPINWPRNMFIWRSNLLGSSGKGHEYFLKHLLGTNHGVLGK
                     DLGETGAQKPKEVVWREEAPQGKLDLLVTLDFRMSTTCVYSDIVLPTATWYEKNDLNT
                     SDMHPFIHPLTNAVDPVWEAKSDWEIYKAIARKFSEIAPEVLGLEKDVVLTPIQHDTP
                     LEIAQAVDVKDWKRGECEPIPGKTMPAVTVVERDYPNIYKQFTALGPLMENIGNGGKG
                     MSWKTEHEVELLKQLNGVVHEEGPSKGLARIDTDMDAAEVILALAPETNGEVAVKAWA
                     SLSNTTGREHTHLALPKEDEKIRFRDIVAQPRKIISSPIWSGLESEKVCYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHLWMRAFGEAFCVYRPPIDTRTVKPVIDTRPNGERQVILNF
                     ITPHQKWGIHSTYTDNLLMLTLSRGGPIVWISEKDAAKAGIVDNDWIEAYNSNGALVA
                     RAVVSQRVKEGMCLMYHAQEKIVNVPGSEITGQRGGIHNSVTRAVLKPTHMIGGYAQL
                     SYGFNYYGTIGTNRDEFVIVRKMRNIDWLDEPAAARPEAAE"
     gene            8092..>9130
                     /gene="narH"
                     /locus_tag="VF226_12175"
     CDS             8092..>9130
                     /gene="narH"
                     /locus_tag="VF226_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX7074791.1"
                     /translation="MKIRAQIAMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNV
                     ETKPGIGFPKDWENQNRWNGGWVRKANGKIEPKIGGKWRVLANIFANPDLPEIDDYYE
                     PFTFDYEHLQNAPELKAFPTARPRSLITGERMEKIEWGPNWEEILGGEFSKRSKDYNF
                     ENVQKEIYGQFENTFMMYLPRLCEHCLNPTCVAACPSGAIYKREEDGIVLIDQEKCRG
                     WRMCVSGCPYKKIYYNWSSGKSEKCTFCFPRIEAGQPTVCSETCVGRIRYLGVVLYNA
                     DGIEAAASVKDEKDLYEAQLGLFLNPNDPQVVAQARRDGVPESWLEAAKKSPIWKMAR
                     EWRVAFPLHPEY"
BASE COUNT         1712 a         2748 c         2826 g         1844 t
ORIGIN      
        1 tcggcgtgct tggggtcttg tatgccttga gcattggcag cttcaccctg tttctgctga
       61 gttttctttt cttgtttttc atgtccggcg tcggcaatgc atcgacgttc cagatgattc
      121 ccgtcatcat gcggctggaa atggacaggc tgatgccggg cgcaacggca gatgagcgtc
      181 agcggcaggc ggagaaggaa gccgctgcga tcatcggctt tacatcggcg atcgcggcgt
      241 tcggcgcgtt tttcattccg agcgcgtatg gcgtttctct tgcgctgacc gggggagcgc
      301 aggctgctct ctggggcttt ctgatctact atatttcctg catcgcgctt acttggggtc
      361 agtatgtccg tcccggcggc ttgctgttcg agattgaacg acgccagcag cggcaggcgg
      421 cgtaacggag gaggcgcctt cgcaagagcg cggcgcactc aacctcaggc ctgcttgttg
      481 tcactcgggt aaagctcggg ctcatcgatg agcggattcg ggcgggcgag tgcggtcaga
      541 gctgtcagat cgtcaatccg cacccggcgg ccattcactt tgaccccatg ccgtgcaagc
      601 tctgcaaaat tgcgggacag gttttccggc cgcatgccga gctgagaggc gagcgagcgc
      661 ttttccatcg gaatcgtgaa gatgcccctt ccacccgcct cgttatgggc tttgagaatc
      721 caacttgcca accgctccag gctcggccgc agccgcatgt ttttcagatc cttcacgatg
      781 gcgcgatagc gcacggccag ctcgcgcagt gcggcgcgcg caaatgcggg atcctcctcg
      841 aacacctcgc gcgcggcatc ggccgggatc aaggcgatca gggatggcgt gagcgtgcga
      901 gcggatttca ggtgtggctg atcgaggacg accgcggcca ggatgaacgc tgctggcgct
      961 cgaatgatcg atagcgtcgt ttcccggtca ccatgccgtg cgaactgctc cacggctcct
     1021 tcgagaatga cgtgcaggaa gtccggcctg tcgccttcgt agaccagatc gacgcgatcg
     1081 ggaaagcgct gcaggtagcc tgcagcgacc agcttctcga aactcgcatg atccatgtcc
     1141 cgaaacagcg ggagtcgacg gatgttttca gcgtcgcttg ctcgcaccgc aattcctctt
     1201 cgatccctgt cgtgtccttg cttgacaatt gatcataatc aatcattcaa ttgatcatag
     1261 caaatgcagt tttgtttttg atcacgcgtt gttgatgtcg gcgcaatgaa tcgccgatcc
     1321 ttctccgcta tcttgacctg aatcaagttg ccctgcactg caatagggca aaggtcggtc
     1381 gcgattgccg atgcgccgac cagctcgggg acgacgaatg accgcccatc caaccgccct
     1441 accgggagac cagaaccgcg ctctttggct ctcgacggcg gccttcaccg cctgtttcgc
     1501 cgtatggacc atattctcga tcatcggtgt acaaataaag agcgacctcg atctgaacga
     1561 gactcagttc gggctgctcg tcggtacgcc cattctcatg ggcagcctca ttcgcctgtt
     1621 gctcggggtg tggactgagc agtatggcgg acgcgtggtt tacaccgccg tcatgctgtc
     1681 tgcggcggtc gcgacctggt tgctgacctt tgcctatgat tacccgacat tcctgctggc
     1741 ggcgctcggc gtcggcatcg ctggcgggtc gttcgcagtc ggcgtcgctt acgtcgccaa
     1801 atggtatcct gagcagaaac aaggtacggc gctcggtgtt tttggtgccg gcaatgttgg
     1861 cgcggccgta acgaaattcg tggcaccctt cgtcatggtc gcttatggct ggcaggccgt
     1921 cgccaacatc tgggcgatcg ccctggccat cgtcggcatt ctcttctggg tcttcgccaa
     1981 ggatgagccg cagctcgagg cgcggcgcaa agccggcgtc aagcccgagc cgatgctcag
     2041 cctcatcgcg ccactcaaaa acctgcaggt ctggcggttt tcgctctact atttcttcgt
     2101 cttcggcgcg ttcgttgcgc tagcactttg gctcccgcgg tatctgatcg gcgtctatgg
     2161 cctcgacatc gccaccgccg gcatgattgg cgccttctac tccgtccctg cttcgatatt
     2221 ccgcatctac ggcggccatc tgtccgacaa gtacggggcg cgccgcgtca tgtactggac
     2281 cttcggcgtg tccgtcgctt gcacatttct gctgtcgtat ccgccgacgg agtacgtggt
     2341 cagcagcatg cgcgggccga ttaccttccg cctcgaaacc ggcctcttcg ccttcaccgt
     2401 attgattttc gtgctcggct ttttcatgag cctcggaaag gcggccgtct acaagcacat
     2461 tcccgtctat tatcccaatc atgtgggccc ggtcggcggt gtcgtcggcc ttgtcggcgg
     2521 acttggcggg ttcgtcctgc cgatcgcgtt cgggctgctc aacgacctga cggggctctg
     2581 gcagagctgc ttcatgctgc tgttcgccat cgtcaccgtg gcgctcgtat ggatgcacgt
     2641 gtcgatccgc atcatggagc gcggggtgct cgagccggag ctcaagaagc ttcccgagct
     2701 gccggaaatg caggagatcc acgagccgcg gcatgccggc gtgctggggc cgcatctgat
     2761 agaggactgg cgaccggagg acgaggagtt ctggaagacg aaggggcggg ccattgcgcg
     2821 ccggaacctg attatctcga tcccatcgct gctgctgtcg ttcgcggtgt ggatggtgtg
     2881 gtccgtggtc gtcgccaagc tgccggccgt cggcttcacc tacaccaccg accagctctt
     2941 ctggctggca gcgttgcccg gcctctcggg cgcaacgctg cgcatcttct attccttcat
     3001 ggtgccgatc tttggcggcc ggctgtggac gacgctcgcc acctggtcgc tgctgatccc
     3061 ggcggtcggg atcgggctgg cagtccaaaa cccgaataca ccgtactggg tgcttctgct
     3121 gctggcgctg acgtgcggat tcggcggcgg caatttcgct tcctcaatgg ccaatatctc
     3181 cttcttcttt ccgaaatcgg agaaggggaa tgcccttgcc ctcaatgccg ggctcggcaa
     3241 cctgggcgtg agcgtagtgc agtttctggt gccgatagcc attacggccg gggtgttcgg
     3301 ctggctgggt ggcgatcctg tcatcgtcaa ggaaggcggg cgcgaggttc cgctctggct
     3361 gcagaacgcg ggctatatat gggtgccttt catcgtcgcc tccgcattcg cggcctggtt
     3421 cgggatgaat gacctggcta cggcacgcgc gtcgttcgcc gagcagtcgg tgatcttcca
     3481 gcgcaagcag aactggatca tgtgctggct ctataccggc acgttcggct ccttcatcgg
     3541 cttttcggct ggcttcccgc tgcttgccaa aacccagttt cccaatatcg acgtgctgcc
     3601 ttacgtcttc ctcggtccgc tgattggctc gctctcgcgt gtcttgtcgg gctggctcgc
     3661 tgacaagtat ggcggcggcc gcgtcacctt ctgggtgttc atcgcgatgt cgattggcgt
     3721 gctcggcgtc ctttattccc tgaacgcggg cagctttgtt ggctttttcg ccatgttcct
     3781 cttcctgttc tttgcgaccg gcgtcggcaa tgcctcgacg ttccagatga tcccggccat
     3841 catgcgcaag gaaatggacc ggctgatgcc cgaggcaacg gtggcagaac gtctgcgcca
     3901 ggcagagaag gaaagcgctg ctatcatcgg cttcacgtcc gcaatcgcag cttacggtgc
     3961 cttcttcatt ccgaagggct acggcacctc gattgccttg acaggtggtc cgcaggcggc
     4021 gctcgtcgta ttcctgatct attatgtcac ctgcatcgcc ctcacgtggg ccgtttatac
     4081 gcgccccggg ggcctgctgt acgacgtgga gcgaggcggc ggcatcaaat cgaaaacggc
     4141 ggctgcccgt gcggcccctt gaaccccgga ggacacggat gagccatttt ctcgatcgac
     4201 tgacgttctt caaaaggccg caggaaacct tttccggtgg ccacgggatc accacgcgcg
     4261 aagatcgttc ctgggaagac ggctaccgga agcgctggca gcacgacaaa attgtccgct
     4321 cgacgcacgg cgtgaattgc acgggttcgt gctcgtggaa aatctacgtc aaaggcggga
     4381 tcgtcacctg ggagacgcag caaacggact acccgcgcac gcggccggac ctgcccaacc
     4441 acgagccgcg cgggtgctcc cgcggcgcgt cctatagctg gtatctctac tccggcaacc
     4501 gagtgaaata tcctctggtg cgctcgcggc tgatgcggct gtggcgggag gcgcgcaaga
     4561 cgctgacgcc cgtcgcggcc tgggcatccg tcgtcgagga tgccgaaaag cgtgcctcat
     4621 acgtgaaaaa gcgcggcctt ggcggattcg ttcgcgcaac ctgggatgag gtgacggaga
     4681 tcatcggggc ggcgaacgcc tatacagtgc gcaaatatgg ccccgaccgc attttcggct
     4741 tttcgcccat tccggcgatg tcgatggtct cgtatgccgc ggggtcgcgc tatctatcgc
     4801 tgctcggcgg cgtgtgcatg tccttttacg actggtactg cgatctccct ccatcgagcc
     4861 cgcagacctg gggcgagcag acggacgtgc ccgagtcggc cgactggtac aactccggct
     4921 tcctcatcct ttggggctcg aacgtaccgc agactcgcac gcccgatgcg catttctata
     4981 cggaagcgcg ctatcgcggt gccaaaagcg tcgtcatttg cccggattat tcggaggcct
     5041 cgaaattcgc cgacatgtgg ctatcggtga agcagggcac ggatgccgcg ctcgccatgg
     5101 cgatgggcca cgtcatcctg cgcgagttcc acgtcgaccg gcaagtgccc tacttcgagg
     5161 actacgcccg gcggtacacg gatatgccga tgctggtccg gcttgtgaag cagggcgaca
     5221 atctggtgcc ggagcggatg atccgcgcct ccgatttcga cgatgccatg ggcgagacca
     5281 acaatcccga atggaagacg gtcgcctacg acgagaactc gggcgaaatc gtcgtgcctc
     5341 gggggtcgat tggcttccgc tggggcgaca agggcaagtg gaatctggag gagaaggacg
     5401 caaagggcaa tcccatcaag ctcaggcttt cgctggcgcc catcaaggat gacatcgcgg
     5461 ctgtcgcgtt cccgtacttc ggcaaccgcg agcatgacca cttcacaggc accgaccatc
     5521 caagcattct gccgcgcaac gtgcccgtga agcggctgaa gctgaaggac ggcgaagcgc
     5581 tcgttgcttc cgtctacgat ctgtttatcg ccaactacgc cgtagaccga gggttcggcg
     5641 gcaggcacat ggcgaaatcc tacgacgccg aggagcccta tacgccggcg tgggcggaga
     5701 agatcacggg cgtgccgcgc gacaagatca tccacgtcgc gcgcgagttc gcccgcaatg
     5761 ccgagaagac caatggccgc tcgatggtga tcatcggcgc tggcatgaac cactggtaca
     5821 actcggacat gaattaccgc ggcgtcatca atatgctggt gatgtgcggc tgcgtcggcc
     5881 agtcgggtgg cggctggtcc cactacgtcg gccaggagaa attgcggccg caatcgggtt
     5941 ggctgccgct cgccttcgct ctcgactggg cgcggccgcc gcgccagcag aactcgacgt
     6001 cggcttttta tgcgcatacc gaccagtggc gctatgagac gcttgacgta tccgacgtcc
     6061 tgtcgccgac ggcgcccgcg ggcccgtggg acggcgcgct catcgactac aatgtgcgtg
     6121 cggagcggat gggctggctg ccgtcagcgc cgcaactcca ggccaatccg ctcgacattg
     6181 ccaagaaggc agcggccgca ggcaaggagc cgaaggacta tgtggctgcg gccctgaagt
     6241 ccggtgagct caagttgagc tgcgaggatc cggacaatcc gatcaactgg ccgcgcaaca
     6301 tgttcatttg gcgctccaat ctgctcggtt cctcgggcaa gggacacgaa tatttcctca
     6361 agcatctgct cggcaccaat catggcgtgt tgggcaaaga tctcggggag acgggcgccc
     6421 agaaacccaa ggaggtcgtg tggcgcgaag aggcgccgca gggcaagctg gatttgctgg
     6481 tgacgctcga cttccgcatg tcgaccacgt gcgtctactc cgacatcgtc ctgccgacag
     6541 ccacttggta cgagaagaac gacctcaaca cctccgacat gcacccgttc atccatccgc
     6601 tgaccaatgc ggtcgatccg gtgtgggagg cgaagagcga ctgggaaatc tacaaggcca
     6661 tcgcacgcaa gttctccgag attgcgccag aagtgctggg cctcgaaaag gacgtcgtcc
     6721 tcaccccaat ccagcacgac acaccgctgg agatcgcgca ggctgtcgac gtcaaggact
     6781 ggaagcgcgg cgagtgcgag ccgatcccgg gcaaaaccat gccggcggtg accgtcgtcg
     6841 aacgtgacta cccgaacatc tacaagcaat ttacggcgct cgggccgctg atggagaaca
     6901 tcggcaatgg cggcaagggc atgagctgga agacggagca cgaggtcgag ctcctgaaac
     6961 agctcaacgg cgtcgtccat gaggaggggc cgagcaaagg gctggcgcgt atcgacaccg
     7021 atatggacgc cgccgaagtg atcctcgcgc tggcgccgga gaccaatggc gaggtggcgg
     7081 taaaagcgtg ggcgtcgctc tccaacacca cgggccgcga gcacacacac ctggctctgc
     7141 cgaaggagga cgagaagatc cgcttccgag atatcgtcgc tcagccacga aaaatcatct
     7201 catcgccgat ctggtcgggt ctggaaagcg agaaggtctg ctacaacgcc ggctacacca
     7261 acgtgcacga gctgattcca tggcgcacgc tgtccgggcg ccagcagctc tatcaggatc
     7321 acctctggat gcgtgcgttc ggcgaggcat tttgcgtcta ccggccgccg atcgacacgc
     7381 gcaccgtgaa gccggtgatc gacactcggc cgaatggcga aagacaagtc attctgaatt
     7441 tcattacgcc gcatcagaag tggggcattc attccaccta tacggataac ctgctgatgc
     7501 tcacgttgag ccgcggcggg ccgatcgtgt ggatcagcga gaaggatgcg gcgaaggccg
     7561 gtatcgtcga taacgattgg atcgaggcct acaacagcaa tggtgcgctg gtcgcccgcg
     7621 ccgtcgtctc gcagcgcgtg aaggaaggca tgtgcctgat gtatcacgcg caggagaaaa
     7681 tcgtgaatgt gccgggctcg gagattaccg gccagcgcgg cggcattcac aactcggtca
     7741 cgcgcgccgt gctgaagccg acgcacatga tcggtggcta tgcgcagctg tcgtacggct
     7801 tcaactatta cggcacgatc ggcaccaatc gcgacgagtt cgtcatcgtc cgcaaaatgc
     7861 gcaacatcga ctggctcgac gaacctgctg ccgctcgccc ggaggccgcg gaatgaacca
     7921 gcttgcgcgc caagttcgtc gcccccgggc ttgtccccgg gatccaacta cccgagatcg
     7981 cctagcggtg gccaggtctc gtcagcttca attgaaggga ttcgctgctc ggtggatcgc
     8041 gggcacaagg cccgcgatga cacggtagag caatttgaga aggcaatgct catgaaaatc
     8101 cgcgcacaaa tcgcgatggt gctgaacctc gacaagtgca tcggctgcca tacctgctct
     8161 gtcacttgca agaacgtctg gaccagccgc gaaggcatgg aatacgcgtg gttcaataat
     8221 gtcgagacga aacccggcat cggctttccg aaggactggg aaaaccagaa ccgctggaat
     8281 ggtggctggg tgcgcaaggc aaatggaaag attgagccga aaatcggcgg caaatggcgc
     8341 gtgctggcca atattttcgc caaccccgat ctgccggaga tcgacgacta ttacgagccc
     8401 ttcacgttcg actacgagca cctgcagaac gcaccagagc tcaaggcatt tccgacggcg
     8461 cgtccgcgct cgctgatcac cggcgagcga atggagaaga tcgagtgggg gccgaactgg
     8521 gaggaaatcc tcgggggtga attctcgaag cgatcgaagg attacaattt cgagaacgta
     8581 cagaaggaga tctacgggca gttcgaaaat accttcatga tgtatctgcc caggctttgc
     8641 gagcactgcc tcaacccgac ctgcgtcgcg gcttgcccgt cgggcgccat ctacaagcgc
     8701 gaggaggacg gcatcgtcct catcgaccag gagaaatgcc ggggctggcg catgtgcgtt
     8761 tctggctgcc cgtataagaa gatctattac aactggtcct ccggaaagtc ggaaaagtgc
     8821 acgttctgct tcccgcgcat cgaagcgggc cagccgacgg tttgctcgga aacctgcgtt
     8881 ggccgcatcc gctatctggg cgtggtgctc tacaacgcgg atggcatcga ggcagcggcg
     8941 agcgtgaagg atgagaagga tctctacgag gctcagctcg gtcttttcct caacccgaac
     9001 gatccccagg tcgtcgccca ggcccgcagg gatggcgttc cggagtcctg gctggaagcg
     9061 gcgaaaaagt ccccgatctg gaaaatggcc agggaatggc gggtggcatt tccgctgcac
     9121 ccggaatacc
//
LOCUS       DASVDU010000187        16384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_439715, whole genome shotgun sequence.
ACCESSION   DASVDU010000187 DASVDU010000000
VERSION     DASVDU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439715"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VF226_12180"
     CDS             complement(<1..437)
                     /locus_tag="VF226_12180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7074792.1"
                     /translation="MIGFVVASVALVAWIYLLLGRGGFWRSNVTDEAIAGARGLPHDN
                     EGPPPAVAAIMPARNEHETVGRAVTAVIAQTYKGPLSLIVVDDQSTDGTAAAAQAAAE
                     DCKASRRTVVLSGGPLPPGWTGKLWAMQQGLQHATERGTSPDY"
     gene            complement(434..1489)
                     /gene="hpnA"
                     /locus_tag="VF226_12185"
     CDS             complement(434..1489)
                     /gene="hpnA"
                     /locus_tag="VF226_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HEX7074793.1"
                     /translation="MVEAPSPATSKTEAPGKPESELGNVLVTGATGLVGGAIVRALLR
                     AGYDVHALVRPASNRANLAGYAVTERQGDILDPATVARALEGVRFLVHSAADYRLWAR
                     DPEVIARTNVEGTRIVMEAALEAGVERIVYTSSVATIAPAPDGTPADESRKAEPSQEM
                     GPYKRSKIIAERLVERMVAERRLPAVIVNPSTPVGPGDVRPTPTGRLVLEAARGRMPG
                     FVETGLNVAHVDDVAEGHVLALRKGRVGERYILGGDNVPLSEILGTIAKLSGRRPPRI
                     KIPHAAVYPAAVASEAWARITGREPLITREGLRLSRQHMFYSDAKARSELGYTSRLYS
                     EALADAVSWFAANGQLR"
     gene            complement(1518..3464)
                     /gene="dxs"
                     /locus_tag="VF226_12190"
     CDS             complement(1518..3464)
                     /gene="dxs"
                     /locus_tag="VF226_12190"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7074794.1"
                     /translation="MPVTPILDQIRCPADLRKLADHQLKALADELRTETIHAVSETGG
                     HLGAGLGVVELTIALHKVFDTPRDRLIWDVGHQSYPHKILTGRRDRIRTIRQGGGLSG
                     FTKRAESEYDPFGAAHSSTSISAGLGMAVARDLAGKKHNVVCVIGDGAMSAGMAYEAM
                     NNAGAMDSRLIVILNDNNMSIAPPVGALSAHLARLISSNAYRQVRGRLKQFADKMPPS
                     WQRKAARAEEFTRNFWTGGTLFEELGFYYVGPVDGHNFDHLLPVLRNIRDAEEGPILV
                     HVVTKKGKGYVPAENSADKFHGVSRFNVVTGAQEKGRPAAPSYTKVFAETLIKLAERD
                     DKIVAITAAMPSGTGLDLFAKKFPRRSFDVGIAEQHAVTFAAGLAAEGYRPFAAIYST
                     FLQRAYDQVVHDVAIQKLPVRFAIDRAGLVGADGPTHAGAFDITYLGCLPGFTLMAAA
                     DEAELVRMLATAAAIDDGPSAVRYPRGDGIGVPLPDPALPLEIGKGRILREGTTVALL
                     SYGARLQECQRAADELQSQGLSTTVADARFAKPLDTDLIRRLAREHEVLVTIEEGAIG
                     GFGSHVLQFLALAGMLDDGLKVRTMVLPDCFIDQDKPEAMYAKAGLNAPGIVRTVMAA
                     LGRAHSQDKAAAGAPLLVAAASRA"
     gene            3973..5376
                     /locus_tag="VF226_12195"
     CDS             3973..5376
                     /locus_tag="VF226_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEX7074795.1"
                     /translation="MFETPNLKLPYIAPAQAQKHVTHNEAIRALDALVHISVEDRDLV
                     APPVEALDGARYIVANGATGEWSGHAQKIAAFQDGAWAFYAPREGWTAWIADEDLLVV
                     WNGEAWMPAAGVTDPTPQLGINATADATNRLAVASPASLFNHEGGGHQLKINKASDAE
                     TGAILFQTNWSGRAEMGLAGDDDFHFKVSPDGSAWHEAIVIERTTGRVRLPATPVREI
                     LAADRTYFVDPTGSDANDGLTAATPFLTIQKAIDAALNLDGAGHTVTIQLADGVYTSG
                     GRISRPMFDGGQLNIIGNPAAPGNVEVAVTGANAFLVDAAGAKVRLEGVKISGDVGVW
                     ARYGAAVFLTGKNAFGACSFRHIGADNGAFVEMLGGEIAIEGAAPHHLYADAGGHIFC
                     ALGTTTISGSPAFPFGFAYAQSSGLVTSYGMTWSGSATGPRYQAMLNGVINANASGPD
                     YFPGDSAGVLASGGQYV"
     gene            complement(5401..6660)
                     /locus_tag="VF226_12200"
     CDS             complement(5401..6660)
                     /locus_tag="VF226_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074796.1"
                     /translation="MLIPLIAACSEGAGLDSGLIQASMWSAEIRTDASAVHPIRAFVL
                     RHAPYKQVDPQTGNPNKNIGRTPGSACEVIRFLAVLARTDPSDSEARTKAEELARWVI
                     SLQDARSGSPTYGGVPSTPDLPAPANRYYYTIDAAFCGNAMFALYDLSKDDRYRQSGL
                     RFADFLVRMQSGPGRPYARPAGAPDGFCEFIVNSGPTPAWNCDRHVKALIALPVLRQA
                     AILSGKPHYEASARSAREFLVEGLSGAWEYAEAAVLAQCRERACPQAWRRVKGPKGEP
                     DTYVYGDTLAYGLRGLFESEGPSLTVRTLYDQFAGYKGKDAKTRQYDGRIAFAGYMHP
                     ASASPDPSSAYYDLVTLGILHGVRLAVRPDHHAVADRVLRSKLATAAQLSWKMNFDLS
                     LPRGEFVDLTTLANLGEALVGPAAGSH"
     gene            complement(6844..7764)
                     /locus_tag="VF226_12205"
     CDS             complement(6844..7764)
                     /locus_tag="VF226_12205"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7074797.1"
                     /translation="MRAVAIINAKAGAVLESGPAIAETIARRFRERGHDLELSLVSPE
                     DIEEALRRAADRSDIEAIIVGGGDGSQSLAASLLAGTGMILGVLPLGTVNLLARDLEV
                     PLDLLSAVDALAGANVENIDLGEVNGRTFHSLAGLGFLARMARERQRARADIPYARWL
                     AFLLALIRAVARVDRMSLWIETENGKLLRRSSAVLVTNNIYRESEWTRPRISDGLLEV
                     HIVRGWTWLPLVKAGFDMMLGRWRRTGRIESILAREIEINPRRRRIPVSIDGEVVQER
                     APLHFRVQRGALKVLRPVYEPPEAGRSPAA"
     gene            complement(7919..8656)
                     /locus_tag="VF226_12210"
     CDS             complement(7919..8656)
                     /locus_tag="VF226_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7074798.1"
                     /translation="MTKRLEGRIALITGASRGIGRAVALAFAREGAHVVLLARTQGAL
                     EEVDDEVREIGGSATLLQLDLRNGDKLDALGPTLYQRWGKLDILVGNAGILGPLSPLG
                     HITADAWSDVITINLTANWRLIRTCDPLLRRSDAGRAIFVTSGAAAGDHAYWGPYAVS
                     KAGLEALVKTYAREMERTPVRANLVDPGGVRTAMRAKAYPGENPASLPTPEEVAPLFV
                     ELASPECTLNGEVISFREWRAAKSQAT"
     gene            complement(8718..9896)
                     /locus_tag="VF226_12215"
     CDS             complement(8718..9896)
                     /locus_tag="VF226_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074799.1"
                     /translation="MIAKQLITAAVFASALAVAAAQGLAGRINTDEEGGTYNDRFCPI
                     LADQLSRSDFSYSCEPSSGTAENIRRVMTDPSELGYGQLDVFASKLANADDAGALVPV
                     RTDDVRQCLFAVTKRRDLQSFGDLSGRSTELNFHLPPQQSGSADTFAILQQIDPTGLG
                     KASIVKHEASPDDAIRSALSEENGVAFFVDFPDPDAPRFALVRELGGHYVPIIDREIL
                     RQQIGGQKIYFAQETQVANADWLTTARKVDTACTPVVLFTGAPEKIADPEARKDHEDL
                     IATIKALRTEALLPDESTIARVIARTKELSATSAERLMQASEKAREQARPYIDRAKEA
                     TEKAVEAAKPALEKAKEYGIRFYEKAREGLKELMGTKPEDGQPSGDEPAPDAPQEQPR
                     "
     gene            complement(10015..11508)
                     /gene="purF"
                     /locus_tag="VF226_12220"
     CDS             complement(10015..11508)
                     /gene="purF"
                     /locus_tag="VF226_12220"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7074800.1"
                     /translation="MAGKADSDLELDDPHWLRYGDDRLREECGVFGVIGHADAAALTA
                     LGLHALQHRGQEAAGIVTFDGQQFHSERRLGLVGDNFNKIEVIKRLGGHMAVGHVRYS
                     TTGETILRNVQPLFADLEVGGFAVGHNGNLTNALTLRRELVQSGAIFQSTSDTEVILH
                     LIARSQKRRIVERLIDALRQLEGAYAFVCLTNDMLIGARDPVGIRPLVIGRIGSAYVL
                     ASETCALDIVGAEFVREVENGEVVVITAERGLESHRPFPMRPARPCIFEYIYFARPDS
                     IVGGRNVYEVRKRMGGELAQEAPAAADVVVPVPDSGVPAAIGYAQASSLPFELGIIRN
                     HYVGRTFIEPEQRIRQLGVKLKHSANSAAIRGKRIVLIDDSVVRGTTSKKIVTMMREA
                     GAREVHMRIACPEIKFGDYYGIDTPTRKELLAASMSVEEMRIFMGADSLQFLSVDGLY
                     RAMGFDGRDSFNPQFSDHCFTGEYPTDLTDLNGGSAPRQLSLLAEAR"
     gene            complement(11571..12212)
                     /locus_tag="VF226_12225"
     CDS             complement(11571..12212)
                     /locus_tag="VF226_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX7074801.1"
                     /translation="MIGPLTYLDVGLLAICFLSGLLAMYRGLTRELLSILSWLAAAAA
                     VLYFVLFHRPFAEDLAQQINAPVSIAQIAIGGIIFLIVLVVVHLITARISDTILDSRV
                     GMIDRILGFLFGVVRGFVLVVIPYMFYESFVPDPQQQVPWVREAKSLPMIRSTGETFR
                     IILTQYAPSSLIEPSGFDQQGATTSPKIQDVAVLPPAEFHISVTQPGSSADRA"
     gene            complement(12262..13641)
                     /gene="radA"
                     /locus_tag="VF226_12230"
     CDS             complement(12262..13641)
                     /gene="radA"
                     /locus_tag="VF226_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7074802.1"
                     /translation="MAKAAKATFVCQNCGAVSTRWTGKCASCEEWNTLVEETVPGPAP
                     PGTGLAASSRGRVVALEPLSGSTEAAPRLPTGLEELDRVTGGGIVPGSALLIGGEPGI
                     GKSTLLLQLCASIGSRGQRAVYFSGEEAVAQIRLRAERLGLSHAPVALASETNLANIL
                     ATLADGRRPDLVVIDSIQTLWADALEAAPGTVSQVRAATQALIRFAKSYGSALLLVGH
                     VTKEGQIAGPKVVEHMVDTVLYFEGDRGHPFRILRAVKNRFGATDEIGVFEMVGQGLR
                     EVANPSELFLGDRDAHTPGSAVFAGIEGTRPLLVEIQALVAPTSLGTPRRAVVGWDAN
                     RLSMLLAVLDARCGVSFAQNDVYLNVAGGLKITEPAADLAAAAALLSSISGIPLPQNQ
                     VFFGEISLSGAVRAASHMTTRLKEAQKLGFGQAVLPQAGELDVSALTLSLQRIPHLKK
                     LAEQLAPCD"
     gene            complement(13718..14869)
                     /gene="alr"
                     /locus_tag="VF226_12235"
     CDS             complement(13718..14869)
                     /gene="alr"
                     /locus_tag="VF226_12235"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7074803.1"
                     /translation="MDARTARQQEDRIPVNARAVLRIDLDALRANWRKLNEASGTAEC
                     AGVIKANAYGLGIEEICRALTEEGCRTFFCATIEEGRQIRTVQPGAVVYVLDGLLPGA
                     AEHYSGFDLRPALSSLLEIREWAAYCRDRGQRLSAAIHIDTGMNRLGLPEYEVKELAA
                     NPEILKDFHVSLIMSHLACADVPESPMNAAQRQKFERLTAMLPPAPRSLANSGGIFLG
                     PDYHYDLVRPGIVLYGGRAHEKGPSTMRTVVRLSAKVLQVFEAQPGETVGYGAAHVID
                     RPMRLATIACGYADGFLRALSGSVGKPGPVGFIGPYPVRVIGRVSMDLITLDVSNVPA
                     DQVYRGAWVEVMGDRVTLDDLTDRAGTIGYELLSRLGRRVHRIYEEGKA"
     gene            complement(14887..16365)
                     /locus_tag="VF226_12240"
     CDS             complement(14887..16365)
                     /locus_tag="VF226_12240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7074804.1"
                     /translation="MRAQAAADEPLEFRQPPHNIEAEQALLGAILVNNEALDRVAGFL
                     EAHHFYDPIHQQIYEVASKLIQAGKQATPITLRTFFENAEPIDDDLTVPQYLGRLAAS
                     ATTIINAADYGRTIYDLATRRALISIGEDLVNTAYDSPVDFPPEEQIQEAEGRLYALA
                     ESGKYGQGFLSFGTALTQAIEMANNAYQREGHLSGLSTGLTDLDAKLGGLQNSDLIIL
                     AGRPSMGKTALATNIAYNVAKAFRAEKQPDGTEKVIDGGVVGFFSLEMSAEQLATRIL
                     SEQAEISSEKIRRGMITEDEFRRLVRVSQEMSHIPLYIDQTGGISVAQLAARARKLKR
                     QRGLGLLVVDYLQLLTGSSKRALEGRVQEVSEITTGLKALAKELNVPVLALSQLSRAV
                     EQREDKRPQLADLRESGSIEQDADVVMFVFREEYYVERRKPSESNVEELLKWQQEMTH
                     VHGKAEVIIGKQRHGPTGTVQLSFESNFTRFGNLAREYHLPE"
BASE COUNT         2857 a         5288 c         5355 g         2884 t
ORIGIN      
        1 atgtaatcgg gcgaggtccc ccgttccgtc gcgtgctgga ggccctgttg catggcccag
       61 agcttgcctg tccagcccgg cggaagcggt ccaccgctga gcacaactgt tcttcgggag
      121 gctttgcagt cttcggcagc agcctgagct gcagccgcgg ttccgtcggt gctctgatcg
      181 tcgaccacga tgagcgacaa tgggcccttg taggtttgcg caataacggc ggtcacggcc
      241 cttccgaccg tctcgtgctc gttgcgggcc ggcatgatcg cggcgaccgc tgggggaggc
      301 ccctcattat cgtgtggtaa gcctcgtgcc ccagcaatcg cctcatccgt gacgttcgag
      361 cgccagaacc cgccacggcc gagcagaaga taaatccagg cgacgagcgc cacgctggcg
      421 acaacgaagc cgatcatctg agctgcccat tcgctgcgaa ccaggacacg gcatccgcaa
      481 gcgcctcgga atagagcctc gacgtgtagc cgagctcgga gcgcgccttt gcgtcgctgt
      541 agaacatgtg ctggcgcgaa aggcgcagcc cctcccgcgt gatcaacggc tcgcgtcccg
      601 taatgcgagc ccacgcctcg gaagcgactg cagcggggta gaccgcagcg tgcgggattt
      661 tgatcctggg cggccgccgc ccgctcagct tcgcgattgt gccgaggatt tcggaaagcg
      721 ggacgttgtc gccaccaagg atgtagcgct ccccgacccg gcccttgcga agcgcgagca
      781 catggccttc ggcgacgtcg tcgacgtgag cgacattgag gcctgtctcg acgaatcccg
      841 gcatgcggcc gcgcgcggct tcgagcacca gtcggccggt cggcgtcggc ctgacgtcgc
      901 ctggtccgac cggcgtgctc ggattgacga tgaccgccgg caaacggcgt tccgccacca
      961 tgcgctcgac cagccgctcg gcgatgatct tgctgcgctt gtacggcccc atctcctggc
     1021 tgggttctgc cttgcggctc tcgtcggcag gcgtaccatc cggagcgggc gcgatcgtcg
     1081 cgacgctgct ggtgtagacg atgcgctcga ccccggcttc gagcgctgcc tccatgacga
     1141 tgcgcgtgcc ctcgacgttg gtgcgcgcga tgacctcggg atcccgtgcc cacaggcgat
     1201 aatccgcggc cgagtgcacc agaaaccgca cgccctcgag cgccctggcg acagttgccg
     1261 gatcgaggat atcgccctgc cgctccgtaa cggcgtagcc ggccagattg gcacgattgc
     1321 tggctggccg gaccaaggcg tggacgtcgt agccggccct gaggagtgca cggacgatcg
     1381 cgccaccgac gaggcccgtt gcgcccgtta cgagcacgtt tccaagctcg ctttcgggct
     1441 tgcccggtgc ctcggtcttc gatgtcgcgg gggaaggggc ctctaccatc atgacgcctc
     1501 accggggcgg gccgccgtca tgcccgggat gctgccgcga cgagcagcgg cgctccggca
     1561 gccgccttgt cttggctgtg agccctgccc agcgctgcca tgaccgtgcg cacgatcccc
     1621 ggcgcattca gccccgcttt cgcgtacatc gcttccggtt tgtcctggtc gatgaagcaa
     1681 tcggggagga ccatcgtccg caccttgagg ccgtcgtcga gcattccggc caaggccaag
     1741 aattgcagca catgggagcc gaacccgccg atcgcgccct cttcgatcgt caccagcacc
     1801 tcgtgctcgc gcgcgagccg gcggatcaag tccgtgtcca acggtttcgc gaaacgggcg
     1861 tcagccacgg tcgtcgacag cccctggctc tggagctcgt cggcagcgcg ctggcattcc
     1921 tgaaggcggg ctccgtaaga gagcagtgca accgtcgttc cttcgcgcag gatgcggccc
     1981 ttgccgatct cgaggggcag ggccgggtcg ggaagaggaa cgccaatgcc gtctccgcgg
     2041 ggatagcgca ccgcggaagg tccatcatcg atggcagccg cggtcgcgag catgcggacg
     2101 agctcggcct cgtcggctgc cgccatcaac gtgaaccccg ggaggcagcc gaggtaggtg
     2161 atatcgaagg caccggcgtg ggtcgggcca tccgcaccga ccaggccggc ccggtcgatc
     2221 gcgaaccgca cggggagttt ctggatcgcg acatcgtgca cgacctgatc gtaggcgcgc
     2281 tgcagaaacg tcgagtagat cgccgcgaac gggcggtagc cctcggctgc cagtccggcg
     2341 gcgaaggtta cggcgtgctg ctcggcgatc ccgacatcga aggagcgtcg agggaacttc
     2401 tttgcaaaca gatcgaggcc ggtaccggac ggcatcgccg cggttatggc gacgatcttg
     2461 tcatcgcgct ctgcgagctt gatcagggtc tcggcgaaga ccttggtgta gctcggcgct
     2521 gcggggcggc ccttctcctg ggcgccggta acgacgttga agcggctgac gccgtggaac
     2581 ttgtccgccg aattctccgc gggaacgtag cccttgccct ttttggtgac gacgtgcacc
     2641 aggatcggcc cttcctctgc gtcccggata ttccgcaaca ccggaagcag gtgatcgaag
     2701 ttgtggccgt cgaccgggcc gacgtagtag aagcccagct cctcgaacaa ggtcccgccg
     2761 gtccagaagt tgcgcgtgaa ctcctccgcg cgggcggctt tgcgctgcca gctgggcggc
     2821 atcttgtcgg cgaactgctt cagacgcccg cgcacttggc gataggcgtt gctcgaaatc
     2881 agtcttgcca gatgcgcgct cagtgcgccg acgggcggcg cgatcgacat gttgttatcg
     2941 ttgagaatga cgatgaggcg cgaatccatc gcgccggcat tgttcatggc ctcgtaggcc
     3001 atgccggcgc tcatggcgcc atcgccgatc acgcatacca cgttgtgctt tttgcccgcg
     3061 aggtcgcgcg caaccgccat tccgagcccc gccgatatcg atgtcgacga atgtgcggcg
     3121 ccgaacgggt cgtattcact ttccgcccgc ttggtaaatc cggaaaggcc gcctccctga
     3181 cggatggtgc ggattcgatc tcgtcggccg gtcaggatct tgtgtggata gctctgatgc
     3241 ccgacatccc agatcagtcg gtcgcggggc gtatcgaaca ccttgtgcag cgcgatcgtc
     3301 agctcgacga ctccgagccc ggccccgaga tggccccccg tttccgacac agcatggatc
     3361 gtttcggtcc gcagctcatc agccaacgct ttgagttgat ggtccgcgag tttccggagg
     3421 tctgctgggc aacggatttg atcgagaatg ggggttaccg gcatgaacga gagccctgtc
     3481 gcaagacgct cgtggagacc tcgagagatc accgcgcgcg ggaatttcgg cctagctcat
     3541 aagcggcccg accatctaaa cgtgggctga gtggaatagt tcgccccgcg accgccaact
     3601 ccgaacgaat ttctgcgatt gctgaacgac cgcggcctcc agctcggcca attcggtcga
     3661 tgatttcagc gggctcggga tcccgaaatc gcgggctgcc gtggtcattc gcagcatcct
     3721 gccaggcaat cccccgtcgt accgcctgga agcgaaatcg gcgtcagctc aacggaatgg
     3781 ttaacgccag atccattcag tgcgcgcgct tttccggaat ttcatccgca gtcgaaatat
     3841 tttccgaggc ctacgagaga gctccgggtg ccatgaaatc gcgcgcgtcc gcacgtcggt
     3901 gcacgaagtt atccccgctc tccaaatccg cctcatgctt ggcgggtcgg taccagtcgg
     3961 gagacatgag ttatgttcga gacacccaac ctgaagcttc cctacatcgc gcccgcccag
     4021 gcgcagaagc acgtgaccca caacgaggcg atccgtgcgc tcgacgctct ggtgcacatc
     4081 tccgtcgagg atcgtgatct cgtcgcgccg ccggtcgaag ccctggatgg cgcccgctac
     4141 atcgtcgcaa atggcgcgac gggcgaatgg tccgggcacg ctcaaaagat cgcagccttt
     4201 caggacggcg catgggcatt ttatgcgccc cgggaaggct ggacggcctg gattgcagat
     4261 gaggatctgc tggtcgtctg gaacggcgag gcgtggatgc cggctgcggg cgtgacggac
     4321 ccgacgccgc agctcggcat caatgcgaca gcagatgcaa ccaatcgact cgcggtggcg
     4381 tcgccggctt cactcttcaa ccacgagggc ggcggtcatc agctcaagat caacaaggcg
     4441 agcgatgccg agacgggagc catccttttc cagacgaact ggtccggccg ggcggagatg
     4501 gggctcgccg gcgatgacga cttccatttc aaggtcagcc cggacggcag cgcctggcac
     4561 gaggcgatcg tcatcgaacg cacgacggga cgcgtaaggc tgcccgcgac gccggtccgc
     4621 gagatcctgg ccgctgaccg tacatacttc gtcgatccga ccggatcgga tgccaacgac
     4681 ggcttaacgg cagcgacacc tttcctcacc atacagaagg cgatagacgc tgcactcaac
     4741 ctcgacggtg ccggtcacac ggtcacaatc caactcgccg acggcgtcta caccagcggc
     4801 gggcggatca gccggccgat gttcgacggc gggcaactca acatcatcgg caacccggca
     4861 gcaccgggga acgtggaagt cgccgttacc ggagccaacg cgtttctggt cgacgccgcc
     4921 ggagcgaagg tccgtctcga gggcgtgaag atcagcggtg acgtcggggt ctgggcccgg
     4981 tatggcgctg ccgtgttcct caccggcaag aatgcattcg gcgcctgcag cttccgacac
     5041 atcggtgccg acaacggcgc cttcgtcgag atgcttgggg gtgagatcgc tatcgaaggt
     5101 gccgcgccgc atcatctcta tgccgatgcc ggcgggcaca tcttctgcgc cctcggcacc
     5161 accacgatca gcggcagtcc ggcgtttccc ttcggattcg cctacgcgca atcgtccggg
     5221 ctggtgacgt cctacggcat gacctggtcg ggcagtgcca cgggtccgcg ctaccaggcc
     5281 atgctcaacg gcgtcatcaa cgccaatgcc tccgggccgg actatttccc cggcgacagt
     5341 gccggcgtat tggcgagcgg cggtcagtac gtgtgacact gagggatagc cagccggcgg
     5401 ttagtgcgaa ccggctgccg gtcccaccaa tgcctccccg aggttcgcga gtgtcgtcag
     5461 gtcgacgaac tcgcctctcg ggaggctcag gtcgaaattc atcttccagg agagctgcgc
     5521 ggccgtcgcc agcttcgagc gcagcaccct gtcggcgacg gcgtggtggt cgggccggac
     5581 cgccagccgt accccgtgca aaatgccgag tgtcaccagg tcgtaatagg cgctcgaagg
     5641 atcaggcgag gcgctggccg gatgcatgta gccggcaaac gcgatccggc cgtcgtactg
     5701 gcgcgtcttg gcgtccttgc ccttatagcc cgcgaattgg tcgtagagag tgcgcacggt
     5761 aagggagggc ccctcggact cgaacagacc gcgcagccca taggccagcg tatctccgta
     5821 gacgtaggtg tcaggctcgc ctttgggccc tttgacacgg cgccaggcct gcgggcacgc
     5881 gcgctcgcgg cattgggcca gcactgccgc ctccgcatac tcccatgcgc cggataatcc
     5941 ttcgacaagg aactcgcgtg cagatcgtgc ggaagcttcg tagtgcggct tgccggagag
     6001 gatcgccgcc tgccgtagaa ccggcaatgc aatgagcgcc tttacgtggc gatcgcagtt
     6061 ccaggcgggc gtggggccgc tgttcacgat gaactcgcag aagccatccg gagcgccggc
     6121 aggtcgcgca tagggccgcc ccggtccgct ctgcatgcga accagaaaat ccgcgaaccg
     6181 caagccggat tggcgataac gatcgtcctt gctcaggtcg taaagagcga acattgcgtt
     6241 gccgcagaac gccgcgtcga tcgtgtagta atatcggtta gcgggagcag gcaggtcggg
     6301 tgtcgatggc acgccaccat aggtcggact gccggaccgg gcgtcctgca ggctgatgac
     6361 ccagcgggcc agctcctcgg ccttggtgcg tgcttccgaa tccgaaggat cggtgcgggc
     6421 cagcactgcc aggaagcgga tgacctcgca ggccgagccc ggcgttctgc cgatattctt
     6481 gttgggattg cccgtctgcg gatcgacctg cttgtagggt gcgtgccgca gcacgaacgc
     6541 gcgaatcggg tgaacggcgc tcgcatcggt gcggatctca gcgctccaca tgctggcctg
     6601 gatcaagcca gagtccaatc cagccccttc ggagcaggcg gcgatgagcg ggatcagcat
     6661 gaggatcgcg ctccacgtcc gcgttttcct tttcgtgccc tgcggtgctg cccgcagccc
     6721 cccagatccg gcgctcaccg cgtcctgccc ccggcgcgtg cggttacgtc tctgttgaac
     6781 gatgttcaca cgcacatgcg gccattggca actcccaatg catcgcaggc gtcggttagc
     6841 gtttcaggcg gctggcgatc gtcctgcttc cggcggttca tagaccggcc gcaagacctt
     6901 gagcgctccg cgttggaccc tgaaatgcag tggcgcccgc tcctggacca cttccccgtc
     6961 gatggagact gggatgcgcc ggcggcgagg attgatttcg atttcccggg ccagaatgga
     7021 ttcgatgcga cccgtgcggc gccagcgccc aagcatcatg tcgaaccccg ccttgaccag
     7081 cggcagccac gtccagcccc tgacgatgtg cacctcgagc aggccatcgc tgatgcgggg
     7141 ccgggtccat tccgactcgc gatagatgtt gttggtcacg agcaccgccg aagatcggcg
     7201 caagagcttg ccgttctccg tctcgatcca gagcgacatg cggtccacac gggcaaccgc
     7261 ccggatcagc gccagcagga aagcgagcca gcgtgcgtag ggaatgtcgg cgcgcgcccg
     7321 ctgacgctcc cgggccattc gggcgagaaa tcccaggccg gcgagggaat ggaaggtgcg
     7381 cccgttgacc tcgccgagat cgatgttctc cacgttcgcc ccggccagcg catcgaccgc
     7441 cgagagcagg tctaagggta cttcgaggtc gcgggcgagc aggttcacgg tgccgagcgg
     7501 cagcacgccg aggatcatgc ccgtgccggc cagcagcgag gcggcgaggc tctgcgagcc
     7561 atcgccgcca ccgacgatga tggcctcgat atccgagcgg tctgctgcgc gcctcaacgc
     7621 ctcctcgatg tcctccggcg agacgagtga cagctcgagg tcgtggcccc gctcccgaaa
     7681 ccggcgcgcg atcgtctcgg caatagcagg ccccgattcc aggaccgcgc cggccttggc
     7741 attgatgatg gcgaccgcac gcaatgatcg ggctccttcg gggccctccc ggtacaagct
     7801 cacgcgatcg ggaaagctct gtttcgatca gaagaacgcc gtcctggcag gccggttcgc
     7861 gtgcggcatg gccggtgctg agcaggcgag cgcagcacaa ttccacgggg ctggtggctc
     7921 aggttgcctg gctcttggcc gctcgccact cccgaaaact gatcacttcg ccgttcaggg
     7981 tgcattccgg gctcgccagc tcgacgaaga ggggggccac ctcttctggc gtcggcaacg
     8041 acgcggggtt ctcgcctgga taggccttgg cgcgcatggc cgtccggacc ccgccgggat
     8101 cgacgaggtt ggcccgcacc ggggtgcgtt ccatctcgcg agcataggtt ttgaccaggg
     8161 cctcgagccc cgctttcgaa acggcgtagg gcccccagta ggcgtggtcg cccgccgcgg
     8221 ctccagaggt gacgaagatc gcgcgaccgg catccgatcg gcgcagcagt ggatcacagg
     8281 tccggatcag acgccagttg gccgtgagat tgatggtgat gacatcggac caggcgtcgg
     8341 ccgtgatgtg ccccaatggc gacaacggcc caaggatgcc ggcattgccg accaggatgt
     8401 cgagcttgcc ccagcgctgg tacagggtgg ggccgagcgc atcgagcttg tcgccgttgc
     8461 gaagatcgag ctggagcagc gtcgccgagc cgccgatttc ccgcacctcg tcgtcgacct
     8521 cctcgagcgc accctgggtg cgggcgagca gcacgacgtg tgcgccctcg cgggcaaaag
     8581 cgagggccac ggcacgcccg attcctcgcg aggcgcctgt gatcagggca atgcggcctt
     8641 caagacgttt ggtcatctgc tcggctgctc tcccgtagct cgatccaaag gctgtcgaac
     8701 gtgtataacg cgcttgtcta cctgggctgt tcctgaggcg catcgggcgc cggctcatcg
     8761 ccggaaggtt gcccgtcctc cggcttcgtt cccatgagct ccttcaggcc ctcgcgcgcc
     8821 ttctcataga acctgatgcc atattccttg gccttctcca gcgccggctt ggcggcttcg
     8881 acggcctttt cggtcgcctc cttcgcgcgg tcgatgtagg gccgggcctg ctccctggct
     8941 ttctccgaag cctgcatcag cctctccgcg ctcgtggccg acagctcctt cgtgcgggcg
     9001 atgacgcgcg cgatcgtgct ctcgtcgggg agcagtgcct cggtgcgcag ggccttgata
     9061 gtggcgatca ggtcctcgtg atccttccgc gcctccggat cggcgatttt ctccggcgcg
     9121 ccggtgaaga gcacgaccgg tgtgcaggcg gtatcgacct tccgcgccgt ggtgagccag
     9181 tcggcgttcg caacctgcgt ctcctgggcg aaatagatct tctgccctcc gatctgctgg
     9241 cgcaggatct cccggtcgat gatcggcacg tagtgcccgc cgagctcccg gaccagcgcg
     9301 aatcgtggcg catcagggtc tgggaagtcc acgaagaacg cgaccccgtt ctcttccgaa
     9361 agcgcgctgc gaatggcgtc gtcagggctg gcctcgtgct tcacgatgga ggccttgccg
     9421 aggccagtcg gatcgatctg ctggaggatg gcgaacgtat cggcgctgcc ggattgctgc
     9481 ggcggtaaat ggaagttgag ctcggttgag cgtccggaga ggtcgccgaa gctctgcagg
     9541 tcgcgccgct tggtgacggc gaacaggcat tggcggacgt cgtccgtacg aacagggacg
     9601 agagcgccgg catcgtcggc gttggccagc ttgctcgcga agacgtcgag ctgcccgtag
     9661 cccagctccg atggatcggt catgacccgg cggatattct ctgcggttcc agaggaaggc
     9721 tcgcaagaat aggaaaaatc ggaccgcgag agctggtcgg cgaggatagg gcagaagcgg
     9781 tcgttatacg ttccgccttc ctcgtcggta ttgatgcgtc cggccaatcc ctgggcggcg
     9841 gcaacggcca gggcggaagc aaacacggcc gcggtgatca attgcttggc gatcatcggc
     9901 aagtctcccc aatcggagca accggacgtg ctctcccaaa cagcttatcc gtttcaggag
     9961 cagatcatac ccgggacgcg tgacacaagg ccggcgggcg ttccggtcgc cggttcagcg
    10021 ggcttcggcg agaagcgaga gctggcgcgg tgccgagcca ccgttgaggt cggtcaaatc
    10081 ggtcggatat tcgccagtga agcagtgatc gctgaactgg gggttgaagc tgtcccggcc
    10141 gtcgaagccc atggcacggt aaagcccgtc gaccgacagg aactgcaagg agtcggcacc
    10201 catgaatatg cgcatttcct cgaccgacat ggaggccgcc agaagctctt tccgtgtcgg
    10261 cgtatcgatg ccgtagtagt cgccgaattt gatctccggg caggcgatgc gcatgtgaac
    10321 ctcgcgcgcc ccggcctccc gcatcatggt cacgatcttc ttcgaggtcg tcccacgcac
    10381 gacgctgtcg tcgatcaata cgatgcgctt gccccggatg gcggcagagt tggccgaatg
    10441 cttcagcttg acgccgagct ggcggatgcg ctgctccggc tcgatgaagg tgcggccgac
    10501 atagtgatta cggatgatcc cgagctcgaa ggggagcgac gaggcctgag catatccgat
    10561 agctgcgggc acgccggagt cgggaaccgg cacgacgacg tcagcggctg ccggagcttc
    10621 ttgcgccagc tcgcccccca tgcgtttgcg cacttcataa acattacgcc cgccgacgat
    10681 cgaatcgggg cgggcgaaat agatgtactc gaagatgcag ggccgggccg ggcgcatcgg
    10741 gaaggggcga tggctctcca acccgcgctc ggcggtgatg accacgacct cgccgttctc
    10801 cacctcgcgc acgaactccg cgccgacgat gtcgagtgcg caggtctcgg aggcgagcac
    10861 gtaggcagag ccgatccggc cgatcacgag cgggcgaatg ccgacagggt cacgcgcgcc
    10921 gatcagcatg tcgttggtca ggcagacgaa agcgtacgcg ccttcgagct gccgcagggc
    10981 gtcgatcaga cgctcgacga tccggcgttt ctgggaccgg gcgatgaggt gaaggatcac
    11041 ctcggtatcg gaggtggact ggaagatggc gccggactgg acgagctcgc ggcgcagcgt
    11101 cagggcgttg gtgagattgc cgttgtggcc gacagcgaag ccgcccacct cgagatcggc
    11161 gaacagcggc tggacgttgc ggaggatcgt ctcgcctgtc gtcgagtagc ggacgtggcc
    11221 gaccgccatg tggccgccca gccgtttgat gacctcgatc ttgttgaagt tgtcgccgac
    11281 gaggccgagc cggcgctccg agtggaactg ctggccgtcg aaggtgacga tgcccgccgc
    11341 ctcctggccg cgatgctgca gggcatgcag ccccagtgcc gtcagggccg ccgcgtcggc
    11401 atggccgata acgccgaaga cgccgcattc ctcgcgtaga cggtcatcgc catagcgcag
    11461 ccagtgggga tcatcgagct ccagatcaga atccgctttg cccgccatcc gaactctccg
    11521 atctgtctcg accacgatgc ctaagacgct accccgagca ccatggcgca tcaggcgcga
    11581 tcagcggagc tcccgggctg cgtgactgat atatgaaatt cagcgggagg caacaccgcg
    11641 acatcctgaa tctttggaga agtcgtcgcg ccctgctgat cgaacccact cggctcgatc
    11701 agtgacgagg gtgcgtattg cgtcagaatg atccggaagg tctcgccagt gctgcggatc
    11761 atcggcagcg atttggcttc gcgtacccag ggcacctgct gctgcgggtc gggcacgaac
    11821 gactcgtaga acatgtacgg gatcacaacg agcacgaatc cgcgcaccac gccgaacagg
    11881 aagcccagga tccgatcgat catgcctacg cggctgtcga ggatcgtgtc ggagatccga
    11941 gccgtgatca ggtggacgac gaccagaacg atcaggaaga tgatgccgcc gatggcaatc
    12001 tgcgcgatgg agacgggcgc attgatctgc tgcgccaggt cctcggcgaa cggcctgtgg
    12061 aaaaggacga aatagagcac cgccgcggcg gcagcgagcc aggacaggat cgacaggagc
    12121 tcccgcgtca ggccgcgata catggccagc aggccggaca gaaagcaaat ggccagaagg
    12181 ccgacgtcga gataggtcag cggcccgatc atggtcgcgg ctccctcagg tacttggcaa
    12241 gcgggacagg tccccacaaa ttcagtcgca cggcgccaac tgctccgcaa gcttcttcaa
    12301 atgtggaata cgttgcaaag aaagcgtcaa cgctgagacg tcgagctcgc cggcctgcgg
    12361 cagaacagcc tgcccaaacc caagcttctg tgcctctttc agcctcgtgg tcatgtgcga
    12421 agcggcccga accgcgccgg aaaggctgat ctcgccgaag aaaacctgat tctgcggcag
    12481 cggaatacct gagatcgacg aaagaagagc ggctgcggcc gccaggtctg cggcgggctc
    12541 ggttatcttc aggccgcccg cgacgttcag atagacgtcg ttctgtgcga agctcacccc
    12601 gcaacgggca tcgaggacgg cgagcagcat cgacaaccgg ttcgcatccc agccgacgac
    12661 ggcccgccgc ggtgtgccga gactggtggg agccaccaaa gcctggatct cgacgagcaa
    12721 tgggcgtgtg ccctcgatcc ccgcgaatac cgccgatccc ggcgtatgtg catcccgatc
    12781 tccgagaaac agctcggacg ggttggcaac ctcgcgcaga ccttgaccca ccatctcgaa
    12841 gacgccgatc tcgtcggtgg cgccaaatcg gttcttgacc gcgcgcagga tgcggaaggg
    12901 gtggccgcga tcgccttcga aatagaggac cgtgtcgacc atgtgctcga ccaccttcgg
    12961 tccggcgatc tgaccttcct tcgtgacgtg gccgacgagc agcaacgcac tgccgtatga
    13021 cttggcgaaa cgaatcagcg cctgcgtcgc tgctcgaacc tggctgacgg ttccgggtgc
    13081 cgcctccagc gcatctgccc agagggtctg gatcgagtcg atgacgacca agtcagggcg
    13141 ccggccatcg gcgagtgtgg caaggatgtt ggcgaggttc gtttcggaag caagagcgac
    13201 gggcgcatgc gacagaccca atctctcggc ccgcaatcgg atttgagcga cggcctcctc
    13261 gccggaaaaa tagacggcac gctggcctct cgagccgatg gaggcgcaga gctgcaacag
    13321 cagcgtcgac ttgccgatcc cgggctcgcc gccgatgagc aaagcagagc ccggcacgat
    13381 cccgccgccc gtcacgcggt cgagctcttc caggccggtg ggaaggcgcg gcgccgcctc
    13441 ggtgctccct gacaatggct cgagggcgac gacgcggccg cgcgaagagg cagccaatcc
    13501 tgttccgggt ggagcagggc cggggacggt ctcctccacg agggtgttcc actcctcgca
    13561 gctggcacat ttccccgtcc agcgggtgct gaccgctccg cagttctggc agacgaacgt
    13621 cgctttggcg gccttcgcca tcagtgctcc acccccgctg ccgcgatcgc agcgccctta
    13681 ggcaagtctc acgagcgaag ggcctgcccg ccagggatca ggccttcccc tcctcataga
    13741 ttcggtgcac acggcgaccg agacgcgaca gcagctcgta gccgatcgtt ccggcacggt
    13801 cggtgagatc gtcgagcgtg acacggtcgc ccatcacctc cacccatgcc ccccggtaga
    13861 cctgatcggc aggcacgttg ctcacatcga gcgtaatgag gtccatcgac acgcggccga
    13921 tgacccgcac gggatagggg ccgatgaaac cgaccgggcc tggtttgccg accgacccgc
    13981 tcaacgccct caagaacccg tccgcatagc cgcaggcgat cgttgcaagc cgcattggcc
    14041 gatcgatgac gtgggctgct ccatagccga cggtctcgcc gggctgggcc tcaaagacct
    14101 gcagcacctt ggcggagagc cgcacgaccg tgcgcatcgt gctcgggccc ttttcatgtg
    14161 ccctgccgcc gtagaggacg atccctggcc gcacgaggtc gtaatggtaa tccggcccga
    14221 gaaagattcc gccggagttt gccaggctgc gcggagccgg cggcagcatc gctgtcagcc
    14281 gctcgaattt ttggcgctgg gccgcgttca tcggactttc cgggacgtcg gcgcacgcca
    14341 agtggctcat tatgagagag acatggaaat ccttcaggat ttccggattg gctgccagct
    14401 ccttgacctc gtactcgggc agccccaacc ggttcatgcc ggtgtcgatg tggatggcgg
    14461 ccgaaaggcg ctgtccgcgg tcgcggcagt aggccgccca ttcccggatt tcgaggaggc
    14521 tcgagagagc tggtcggaga tcgaatccgg agtaatgttc cgccgcgccc ggcagcaggc
    14581 catcgaggac atagacgacg gctcccggct gcacggtacg gatctggcgg ccctcctcga
    14641 tagtcgcgca gaaaaacgtc cggcaacctt cctcggtcag cgcgcggcag atttcctcga
    14701 tgccgagtcc gtaggcgttg gccttgatga cgccggcgca ctcggcagtc ccggatgcct
    14761 cgttgagctt acgccagttg gcccgcaggg catcaagatc gatccgcagc accgcccgtg
    14821 cattgaccgg gattcgatcc tcctgctggc gtgcggtcct ggcgtccatc tcagatcaac
    14881 tcaatcctat tcgggcaaat gatactcccg cgcgagattg ccgaaccggg tgaaattcga
    14941 ctcgaacgaa agctgcacgg ttccggtcgg gccgtggcgc tgcttgccga tgatcacctc
    15001 cgccttgccg tggacgtggg tcatctcctg ctgccacttc aataactcct cgacattcga
    15061 ctcggacggc ttgcgccgtt ccacatagta ctcctcgcgg aagacgaaca tcaccacgtc
    15121 cgcgtcttgc tcgatcgagc ccgactcacg caagtccgcg agctgcggcc gcttgtcctc
    15181 ccgctgctcg accgcgcggg agagctgcga gagcgcaagc accggcacgt tgagctcttt
    15241 cgccagcgcc ttgaggccgg tggtgatttc ggacacctcc tgtacgcgcc cttccaacgc
    15301 tcgcttcgat gagcccgtga ggagctgcag atagtccacg accagcaggc cgaggccccg
    15361 ctggcgcttc agcttccggg cgcgggccgc aagctgcgcg accgagatgc cgccagtctg
    15421 gtcgatgtag agcggaatgt gggacatttc ctggctgaca cggaccagcc gccggaattc
    15481 gtcctccgtg atcatgccgc gccggatctt ctccgacgag atctccgcct gctcggacag
    15541 gatgcgcgtg gccagctgct cggccgacat ctcgagcgag aagaagccga cgacgccgcc
    15601 gtcgatcacc ttctccgtgc cgtcgggctg cttctcggcc cggaacgcct tcgcgacgtt
    15661 gtaggcgatg ttggtcgcca gcgccgtctt gcccatggaa gggcggccgg caaggatgat
    15721 gagatcggag ttctgcaggc cgccgagctt ggcgtcgagg tccgtgaggc cggtggatag
    15781 ccccgaaagg tgcccttcgc gctgataggc gttgttggcc atctcgatgg cctgcgtcaa
    15841 cgccgtgccg aaactcagga agccctgtcc gtacttgccg ctttccgcca gcgcatagag
    15901 tcgcccttcc gcctcctgta tctgttcttc gggcggaaag tcgacagggc tgtcgtaggc
    15961 ggtgttgact aggtcctcgc cgatgctgat cagagcccgc cgggtagcga ggtcgtagat
    16021 ggtgcggcca tagtcggcgg cgttgatgat ggtggtcgca ctggccgcga gccggccaag
    16081 gtactgcggc acggtcaggt cgtcgtcgat cggctccgcg ttctcgaaga acgtgcgcag
    16141 cgtgatgggc gtcgcctgct tgccggcctg gatcagcttg ctcgcgacct cgtagatctg
    16201 ctggtggatg ggatcataga agtggtgggc ctcgagaaag ccggcaacac ggtcgagcgc
    16261 ctcgttgttg accaggatgg cgccgagcag ggcctgctcg gcttcgatgt tgtgcggcgg
    16321 ctgacggaac tccagcggct cgtcggcagc cgcctgggcg cgcagcttgt ctgaagggag
    16381 gaag
//
LOCUS       DASVDU010000188         5442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_622770, whole genome shotgun sequence.
ACCESSION   DASVDU010000188 DASVDU010000000
VERSION     DASVDU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622770"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1247
                     /gene="dctP"
                     /locus_tag="VF226_12245"
     CDS             270..1247
                     /gene="dctP"
                     /locus_tag="VF226_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7074805.1"
                     /translation="MAIAHILPEDMENEVHPALTHFKSLVESGTKGAVEVQIFGGGQL
                     GSEVETAKQAQDGKLLQASVISSGAMSSFYKRYQAVTAPFLFPNFQVAHAFFDSPWFA
                     NFMRGTIEEARLRYLGTFDDGGGFVAFTTNKRLIRTADDIKGLKIRVEENPSHVAVMR
                     ALGASATPLPWGEVITALSTGLADGQFNAPGVSKFFKLWEVNQYTTLSRHVYNTQTWV
                     MSEDWFQSLPEEHRKIIIQAARESIELAHGMSALQAIDGWNESCKRFKECYVLPVEER
                     EKMAEIARPAWKEWIVKDFGLDEALVDGLIAEVGRVAAEVDKANAVYAK"
     gene            1362..1940
                     /locus_tag="VF226_12250"
     CDS             1362..1940
                     /locus_tag="VF226_12250"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7074806.1"
                     /translation="MTPRLARISHALDAAVTVFCVGCVLVMLGISFTGSFYMFFTGDA
                     LSWTYSLARLFIPWLGLLSITVAFRRGEHIAMSSLMEIVPRPIGLAARAANYVIVGVF
                     ALLMIWYGTEYAMNSRDLYMVSDQIQVDARWVASSVPITGIILLVHVLCGVNLFDPPS
                     ILDEGDLEVTHEQLVEAGLAPSGSSQKGASGA"
     gene            1937..3214
                     /locus_tag="VF226_12255"
     CDS             1937..3214
                     /locus_tag="VF226_12255"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7074807.1"
                     /translation="MTSAIGVFIFMLLIGTPIAVVMVMSGLAGAMELGGTNFLGIIAE
                     RMFSGVTSYLLMAIPYFVFTAELMNRAGLTERLIAFNNALLGRIRGALSHVNIVASLF
                     FSGLTGAAITDTVALGKILIPAMKREGYTAEYAAAVTACSSILGPIIPPSIVMVVYAS
                     ILHNVSVLQLFAAGILPGFLMAAALFATSAILAWRHNFPRHGDTNLRAAARAFLPTVP
                     ALLVPVIILGGILAGLTTVTEASAFAAVYTIALGILAYRTLTLRGLWDSLVATVQFSG
                     VVFFLLAASTVLGWFVTRSGIAREAATMIATVSKDPIVQIMLVNALLIVIGMVIDVLP
                     ALVVVAPVLVPAMVQLGFDPLHFAMVMILTLNLANITPPVGMTLMTATQIAGVPYERS
                     IRASVPFYICHLIVIIIVSVWPAFVLWLPNMLK"
     gene            3245..4456
                     /gene="thrC"
                     /locus_tag="VF226_12260"
     CDS             3245..4456
                     /gene="thrC"
                     /locus_tag="VF226_12260"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7074808.1"
                     /translation="MELRCVECGHTMPADMYYACGKCSGILEVIAPIGTSIGSNAYLQ
                     STMWRHADRLSVGTPEAIVSLGEGRTPLHRAERLEQSIPGFTGEIWLKDETVNPTGSF
                     KDRLISAAVSRARELGCPGIVCASSGNAGASASAYAARAGLQAIIVVPTHTPEEKLTQ
                     IAAYGALLVKVDGHYSRSYDVAQRLARDHGLVNVTTTFLNPYGVDALKLAGDELADQL
                     GGRVPDYVTVPTGAGPLVKGVVQGFAERPGRRVPKIVAVQAAGCAPIVRAFDAGAQTV
                     TAWDTPDTIASGISDPLIGYERDGTYTLRLVRQSGGLAVAVTDDQIRAAMAELAHRQG
                     LLAEPTGACSLAGAQALLASGRIPMDAVIVCMVTGHGYKDMKIYRGMPTRVRQLPSEP
                     DDAQLSDLLRA"
     gene            complement(4479..>5442)
                     /locus_tag="VF226_12265"
     CDS             complement(4479..>5442)
                     /locus_tag="VF226_12265"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX7074809.1"
                     /translation="FRNSAVFYGGAIYIFGLSLLIAIFLAWRGRIRIAEGESGETVRR
                     FNVVERANHWLTATSFLLLAITGLIILYGLSVIRPWLGADPYDDVAGFSAWSHVALAV
                     PFVLGVLVMIFLWTRENLPERLDWNWMRQFGGFLNDSAEKPPARRFNAGQKLVFWSVA
                     LSGIVLLVTGLFLMFPFDWAGYTGMQAAQVVHAVVALLMVGVIIGHIYIGTVGMQGAF
                     EAMWSGRVDRNWAKEHHQLWYDRIESGADREAARAAPRSRFSPAAGLATGAVVAVVLA
                     LIMSAVYSDVGVGSAVATSRDNPAVHLGQADLNASSRAFMERMR"
BASE COUNT          998 a         1666 c         1698 g         1080 t
ORIGIN      
        1 cgtctcgaat cttgaacggc ggaacgaggt tggcctggtt aaggaggtgc agccgatcga
       61 ggtagtaatc ctggatcaag aaagtccaga atacggctta agctcagaac gatagtccag
      121 attttgagta ggggagtgaa atgactaagc agggatccgc cgccgacggc ggacgtttcc
      181 gccgctcggc aggtgcggtg gcatgtgcag ctggattgat attcgccagc ggagccagtc
      241 tgactcctgt cctcgcggcg gagtacacga tggccattgc gcacatcctg cccgaggata
      301 tggagaatga ggtccatccg gctctcacgc acttcaagtc gctggtcgag tccgggacaa
      361 agggggcggt ggaggtgcag attttcggcg gcgggcagct cggctcggaa gtcgaaacgg
      421 ccaagcaggc tcaggacggc aagctgctgc aggccagcgt gatctcctcc ggtgccatgt
      481 cttctttcta caagagatac caggcggtca ccgctccctt tctcttcccg aacttccagg
      541 tcgctcacgc tttcttcgac agcccctggt ttgccaactt catgaggggt accattgaag
      601 aggctcggct ccgttatctc ggcacattcg atgacggtgg tggctttgtt gctttcacca
      661 cgaacaagcg cctgatccgc acggcggacg acattaaagg gctaaaaatc cgcgtcgagg
      721 aaaatccctc gcacgttgca gtgatgcgcg cgttgggcgc ttctgcaaca ccgctgccgt
      781 ggggcgaggt cataacggcg ctatccacgg gcctggccga tggccaattc aatgctccgg
      841 gagtgagcaa gttcttcaaa ctttgggaag tcaaccaata cacgacgctc agtcggcatg
      901 tttacaacac ccaaacttgg gttatgagcg aggattggtt tcagtctctt cccgaagagc
      961 accggaagat catcatccag gcggcgcgcg agtcgatcga gcttgcccat ggcatgtcgg
     1021 ccctgcaggc catcgatggc tggaacgagt cctgcaagag attcaaggaa tgctatgtcc
     1081 tgccagtcga agagcgggag aagatggccg agatcgcccg cccggcatgg aaagagtgga
     1141 tcgtcaagga tttcggtctg gacgaagcgc tcgtagacgg cctgattgct gaagtcggcc
     1201 gggtcgcagc cgaggtcgac aaggccaacg ctgtctacgc gaagtgaatc tcatccaccc
     1261 gtgagcgatt cttccagcgg gttccaatgc agtgaagcac caggcagccc tctgttgcct
     1321 ggtgcaaaga ttagcttccg agcagagaaa gattagcgac cgtgacgcct cgcctcgcac
     1381 ggatcagtca tgccttggat gcggccgtta ccgtgttctg cgtcggttgt gtccttgtta
     1441 tgctggggat ctcgttcacc ggttccttct atatgttctt tacgggagat gcgctgagct
     1501 ggacatactc acttgcccgc ctgttcatcc cctggctcgg gcttctcagc attacggtcg
     1561 cctttcggcg cggcgagcac attgccatga gctctctgat ggaaatcgtg ccgcgaccga
     1621 ttggcctcgc agccagggcc gcgaattacg tgattgtcgg cgtattcgct ctactgatga
     1681 tctggtacgg cacggagtac gcgatgaact cgcgggatct ctacatggtg tctgaccaga
     1741 ttcaggtcga cgcgcggtgg gtggccagct ccgttccgat cactggcatc attctgctcg
     1801 tccacgtcct gtgcggggtc aatctgtttg atccgccgag catcctcgac gaaggcgatc
     1861 tcgaggtcac ccatgagcag ctcgtggaag ctggattggc gccgagcggg tcgagccaga
     1921 agggggcatc gggggcatga cgtccgcgat cggcgtcttc atctttatgc ttctgattgg
     1981 cacgccgatt gccgtcgtga tggtgatgtc tggtcttgcc ggcgcgatgg agctcggcgg
     2041 caccaacttc cttggcatca tcgcggagcg gatgttctcg ggggtcacca gctatctgct
     2101 catggccatc ccgtacttcg tcttcacggc cgagctgatg aatcgggcag gcctgactga
     2161 gcgcctcatc gcattcaaca atgcgttgct cggccgtatc cgtggcgcac tgagccacgt
     2221 gaacatcgtc gccagcctgt tcttctcggg gcttaccgga gccgccatca ccgatacggt
     2281 tgcgctcgga aagatcctca ttcccgccat gaagcgggag ggctacacgg ccgaatacgc
     2341 ggcagcggtg acggcctgct cctcgatcct cgggccgatc attccgccca gcatcgtaat
     2401 ggtcgtctac gcatcgatcc tgcacaacgt gtcggttctg cagctctttg cggcgggcat
     2461 tctgcccgga tttctcatgg cggctgcgtt gttcgcgacg agtgcaattc ttgcatggcg
     2521 ccacaatttc ccgcgccacg gtgacaccaa tctgcgtgcg gccgcgcgcg ccttcttgcc
     2581 gacggtccct gcgcttctcg tgccggtcat catcctaggc ggcattctgg ccgggcttac
     2641 gacggtcacg gaggcatccg ccttcgccgc cgtttataca atcgcgctcg gcatcctcgc
     2701 ataccgtacg ctgaccctgc gcgggttgtg ggactcactg gttgcgacgg tgcagttcag
     2761 cggagtggtc ttcttcctgc tggcggcctc aacggtgctc ggatggttcg tcacacgctc
     2821 cggcatcgcc cgcgaggcag cgacgatgat cgcgacggtc agcaaggacc cgatcgtcca
     2881 gatcatgctg gtgaacgcgc tgctgatcgt gatcggcatg gtcatcgacg ttctgccagc
     2941 gctcgttgtc gtcgcgccgg tgctggtgcc ggccatggtg caactcggat ttgacccgct
     3001 gcattttgcg atggtgatga tcctgacgct caacctggcc aacatcacgc cacccgtcgg
     3061 gatgacgctg atgacggcga cgcagatagc gggggtcccg tacgagcgct cgatcagggc
     3121 atcggtgccg ttctatatct gccatctgat cgtgatcatc atcgtctcgg tttggccggc
     3181 gttcgtgctc tggctgccca acatgctgaa atgatgccgg caggctgaag gaggcgcagg
     3241 tcccgtggaa ttgcgttgcg tggaatgcgg ccacaccatg ccggccgaca tgtattacgc
     3301 ctgcggaaaa tgcagtggaa ttctcgaggt catcgcgccg atcgggacga gcattggcag
     3361 caacgcctat ttgcagagca cgatgtggcg gcatgctgac cggctatcgg tcggcacgcc
     3421 ggaggccatc gtgtctctcg gcgagggccg cacgccgtta cacagggcgg agcggctcga
     3481 acaatccata cccggattca cgggcgagat ctggctgaag gacgaaacgg tcaatccgac
     3541 cggctccttc aaggaccgcc tgatcagtgc cgcggtgagc cgcgcgcgtg agcttgggtg
     3601 tcccggcatc gtgtgcgcct cgagcggcaa tgcgggcgcc tcggcatccg cttacgctgc
     3661 gcgtgcgggg cttcaggcga tcatcgtcgt gccgacacat acgccagagg agaagctgac
     3721 acagatcgcc gcttacggcg cgctcctggt gaaagtcgac ggccactaca gccgatccta
     3781 tgacgtcgcg cagcgattgg cacgcgacca cggcctcgtc aacgtcacga cgacctttct
     3841 caatccatat ggtgtggatg cgctaaagct cgccggggat gagctcgccg atcagctcgg
     3901 cggccgtgtt cccgattacg ttaccgtgcc gaccggtgcg ggaccgctgg tgaagggcgt
     3961 cgtgcaaggc tttgccgagc gccccggtcg gcgcgttccg aagatcgttg ccgttcaggc
     4021 cgcgggctgc gcgccgatcg tccgggcctt cgatgccggc gcacagacgg ttaccgcctg
     4081 ggacacgccg gatacgatcg catccggcat ttccgatccg ctcatcggct acgagcgtga
     4141 cggaacgtac acgctgcgtc tcgtgcgcca gtcgggcggt ctggccgttg ctgtaaccga
     4201 tgaccagatt cgcgccgcaa tggcggagct ggcccatcgg cagggccttt tggcggagcc
     4261 aacgggcgca tgctccctcg ccggggcaca ggctctgctc gcctctggcc gcattccgat
     4321 ggatgccgtc atcgtctgca tggtgacggg gcacggctac aaggacatga agatctaccg
     4381 tgggatgcca acccgcgttc gccagctccc gagcgagccc gatgatgctc agctttcgga
     4441 cctgcttcgg gcgtgagcag cccgtgccga gcgggcgatc accgcatccg ctccatgaac
     4501 gcgcggctgg acgcattcaa atcggcttgc ccaaggtgca cggcaggatt gtcccggctg
     4561 gtcgcaacgg cgctgcctac cccgacgtcg ctgtaaacag ccgacatgat aagggcaagc
     4621 acgacggcaa cgacggcgcc tgtcgccaag cctgccgcag ggctaaaccg tgagcggggc
     4681 gcagcacggg ccgcttctct gtccgcgccg gactcgatgc ggtcgtacca gagctgatga
     4741 tgctccttcg cccagttgcg gtcgacgcgc ccgctccaca tcgcctcgaa tgcgccctgc
     4801 atgccgaccg tgccgatata gatgtggccg atgatgacac cgaccatcag tagcgcgacg
     4861 acggcgtgca ccacctgggc cgcctgcatt cccgtgtagc cggcccagtc gaaggggaac
     4921 atcaggaaga ggccggtcac cagaagcacg atcccgctca gcgcaacact ccagaacacc
     4981 agcttctgac cggcgttgaa tctacgggcg ggtggcttct cggcgctgtc gttgagaaac
     5041 cccccgaact gcctcatcca gttccagtcc agccgctcgg gcagattttc cctcgtccaa
     5101 aggaaaatca tcacgagcac gcccagcacg aacggaaccg ccagcgcgac gtgtgaccag
     5161 gcgctgaacc cggcaacatc gtcataggga tcggctccga gccatggccg gatcacgctc
     5221 aggccgtaga gaatgatgag ccctgtgatg gccagcagca ggaagctggt ggccgtaagc
     5281 cagtggttgg cgcgctcgac cacattgaac cggcgaaccg tctcgccgct ctcgccttcg
     5341 gcgatgcgga tgcgaccgcg ccaggcgaga aagatcgcaa tgaggagcga taggccaaag
     5401 atgtagatcg cgccgccgta gaacacggcg ctgttgcgaa ac
//
LOCUS       DASVDU010000189         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_583273, whole genome shotgun sequence.
ACCESSION   DASVDU010000189 DASVDU010000000
VERSION     DASVDU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583273"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /gene="grpE"
                     /locus_tag="VF226_12270"
     CDS             <1..606
                     /gene="grpE"
                     /locus_tag="VF226_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7074810.1"
                     /translation="RVAELEAQVADLTDRLLRAHAEIDNIRKRAERERLDTAKYAITK
                     FAGDIVVLADNFQRAIAVVPERAIEQDTTLKGLVEGVQMMEREFLNVLERHGIKRIDP
                     QGQPFNPHLHDAVMEQQNADVPNGTVVQVFQPGYTIEDRVLRPAMVVVSRGGPKAAPA
                     EKPAEQKADSAAQEPAQAHSTAESGSGASSENAAPDDDQSQ"
     gene            complement(618..1232)
                     /locus_tag="VF226_12275"
     CDS             complement(618..1232)
                     /locus_tag="VF226_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003571375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX7074811.1"
                     /translation="MTLSGFKLLTGYFDAQRQAELLRQVEDVVRGAPFYRPTMPRSGK
                     PLSVEMSNAGKLGWVTDKQGGYRYQRVHPVTGQPWPAIPDMLMGLWKDVAEYPAPPEA
                     CLINLYGPGSKLGSHVDADEKDSKAPVVSVSLGDDAIFHIGGPKRSDPKTRLHLQSGD
                     VVVLGGAARFAYHGVDRILPGTSKLIAGGGRINLTLRRVTPPDS"
     gene            1512..3467
                     /gene="dnaK"
                     /locus_tag="VF226_12280"
     CDS             1512..3467
                     /gene="dnaK"
                     /locus_tag="VF226_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7074812.1"
                     /translation="MREENGDMSKVIGIDLGTTNSCVAVMEGGQPKVIVNAEGMNTTP
                     SVVAFTDDGERLVGLPAKRQAVTNPANTFFAIKRLIGRRYDDPTVEKDKGLVPYSIVR
                     GPNGDAWVATKDGKQYSPQQISAFVLQKMKETAEAYLGHPVSQAVITVPAYFNDAQRQ
                     ATKDAGKIAGLEVLRIINEPTAAALAYGLDKKKESKTIAVYDLGGGTFDISILEIGDG
                     VFEVKSTNGDTFLGGEDFDMKLVNYLADEFKKENGIDLRNDKLALQRLKEAAEKAKIE
                     LSSAAQTEINLPFITADQSGPKHLTMKLTRAKLESLVEDLIARTRPPCDQALKDAGLK
                     AAEIDEVVLVGGMTRMPRVQQVVKEMFGKEPHKGVNPDEVVAVGAAIQGGVLKGEVKD
                     VLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDGQTAVTIKVYQGERE
                     MAADNKHLGTFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSAKDKATGKEQSIRIQAS
                     GGLSDEDIQRMIKEAEAHASEDKRRRELVEAKNHLEALIHSTERSVSEAGDKLPAAVK
                     TDIESAITSAREAAQSDDLERIKSATQSLAQAALKIGEAVYGGQQPGGAEAGAPEGEP
                     GKGGPGAEDVVDAEFEEVKDNNKKDSA"
BASE COUNT          761 a         1158 c         1168 g          545 t
ORIGIN      
        1 cgggtggcgg agctcgaggc gcaggtggcc gatttgacgg atcgtctgct tagggcacac
       61 gcggaaattg acaacatccg caaacgcgcc gagcgcgaga ggttggacac ggccaagtac
      121 gcgatcacca agttcgccgg cgacatcgtg gtcctggcgg acaacttcca gcgggcgatt
      181 gccgtcgtgc cggagcgcgc gattgagcag gatacgacgc tcaaggggct cgtcgagggc
      241 gtccagatga tggagcgcga attcctcaac gtgctcgagc ggcacggtat caagcgcatc
      301 gacccgcagg ggcagccctt caacccgcat ctccacgacg ctgtcatgga gcaacagaac
      361 gccgacgtgc ccaacggcac ggtcgtgcag gtcttccagc cgggctacac gatcgaggat
      421 cgcgtgctgc gaccggccat ggtcgtcgtc tcgagaggag gccccaaggc agccccggcg
      481 gaaaagcccg ctgagcaaaa agcggactct gctgctcagg agccggcgca ggcccacagc
      541 acggctgaat cgggatcggg cgccagttca gaaaatgccg cgccggacga cgatcaatcc
      601 caataagggc acccaattca agaatctggc ggcgtcacgc gccgcaaggt cagattgata
      661 cggccgccgc cggcgatgag ctttgaagtt cctggcagaa tgcggtcgac cccgtggtag
      721 gcgaagcggg cggcgccgcc gagcacgacg acatcgcccg actgaaggtg cagccgcgtc
      781 ttcggatcgg accttttcgg tcccccgatg tggaagatgg cgtcatcgcc aagcgagacc
      841 gatacgacgg gcgccttcga atccttctcg tcggcgtcga cgtgactgcc gagctttgac
      901 ccggggccat aaaggttgat caggcaagcc tcgggcggag ctgggtactc cgccacatcc
      961 ttccataaac ccatcagcat gtccggaatg gctggccagg gctgccctgt caccgggtgt
     1021 acccgctggt agcgatagcc gccctgcttg tccgtcaccc agccgagctt gcccgcgttg
     1081 ctcatctcca ccgacagcgg ttttccgctc cgaggcatgg tcggccgata gaagggcgcc
     1141 ccgcggacca cgtcctcgac ctggcgcaac agctcagcct ggcgctgtgc gtcgaaatac
     1201 ccggtgagaa gtttgaagcc gctcagcgtc ataccggcct gcccaccgca cttgcgcacc
     1261 gcacttgaaa aaggctacat ccgccgccgc agcagccgac gcttcgacag gtggggccat
     1321 tatgcaatgg cgacgcaagt tcgccatttt tgtaacaaac gcgtcgcgca ctgccgttgc
     1381 agccccaatc acccccgctt atatacccgc ttgacctact acaagagctc caggggaccg
     1441 tctcaccaac cccgcgcggg aagggggcat gaccgggcgc cgaaagaccg ggtccgggcg
     1501 gtcagccgca gatgagagag gaaaatggcg acatgtcgaa agtgattgga attgacctcg
     1561 gaacgacgaa ctcgtgcgtg gccgtgatgg aaggcggtca gccgaaggtc attgtcaacg
     1621 ccgaaggcat gaacacaacc ccgtcggttg ttgcgtttac ggatgatggg gagcggctcg
     1681 tcggtcttcc ggcgaagcgt caggccgtca ccaacccggc caacaccttc ttcgccatca
     1741 agcgcctcat cgggcggcgc tacgatgatc ccactgtcga gaaggacaag gggctcgtgc
     1801 cctacagcat cgtgcggggc cccaacgggg acgcgtgggt tgcgaccaaa gacgggaagc
     1861 agtactcccc gcagcagatt tcggccttcg tcctccagaa gatgaaggag acggccgagg
     1921 cctacctcgg tcatcccgtt tcccaggccg tgattacggt cccggcctac ttcaacgacg
     1981 cccagcgcca ggccaccaag gatgccggca agatcgccgg cctcgaggtg ctgcgcatca
     2041 tcaacgagcc gacggcagcg gcactcgcct acggtctcga taagaagaag gagagcaaga
     2101 cgatcgccgt ctacgacctc ggcggcggca ccttcgatat ttcgattctc gagatcggcg
     2161 acggcgtctt cgaggtgaag tcgacgaatg gcgacacctt cctcggcggc gaagacttcg
     2221 acatgaagct cgtcaactat ctcgccgacg agttcaagaa ggagaacggc atcgacctgc
     2281 gcaacgacaa gctcgccctg cagcgcctca aggaggccgc cgagaaggcg aagatcgagc
     2341 tgtcgtcggc tgcccagacc gaaatcaacc tgccgttcat caccgcggac cagtctggtc
     2401 ccaagcacct gacgatgaag ctgacccgcg ccaagctgga aagcctcgtc gaggacctga
     2461 ttgccaggac ccgcccgccg tgcgatcagg cgctgaaaga tgccggcctc aaggccgctg
     2521 aaatcgatga ggttgtcctc gtcggcggca tgacccgtat gccgcgcgtg cagcaggtcg
     2581 tgaaggagat gttcggcaag gagccgcaca agggcgtcaa ccccgacgag gtcgttgctg
     2641 ttggcgcagc gatccaaggc ggtgtcctca agggcgaagt caaggacgtt ctcctgctcg
     2701 acgtgacgcc gctgtcactc ggcatcgaga cgttgggtgg tgtgttcacc cggctcatcg
     2761 accgcaacac gaccatcccg accaagaaga gccaggtgtt ctcgaccgcc gaggacggcc
     2821 agaccgcggt gaccatcaag gtctaccagg gcgagcgcga gatggcggcc gacaacaagc
     2881 acctcggcac gttcgacctg gtcggcatcc cgccggcgcc gcgcggcgtg cctcagatcg
     2941 aggtgacgtt cgacatcgac gccaacggca tcgtcaacgt ctccgccaag gacaaggcga
     3001 ccggcaagga gcagtcgatc cgcatccagg cttcgggtgg cctcagcgac gaggacatcc
     3061 agcggatgat caaggaggcc gaggcgcacg cgagcgagga caagcggcgg cgcgagctcg
     3121 tcgaggcgaa gaaccacctc gaggcactta tccactccac ggagcgctcc gtttccgagg
     3181 ccggcgacaa gcttccggct gccgtgaaga ccgacatcga atcggcaatc acctcagcgc
     3241 gcgaggctgc ccagagcgac gacctcgagc ggatcaagtc ggcaacccag tcgctggcac
     3301 aggccgcgct caagatcggc gaagccgtct acggcggcca gcagcctggc ggtgccgagg
     3361 ctggcgctcc cgagggagag cctggcaagg gcggaccagg agcggaggac gtcgtcgacg
     3421 ccgagttcga agaggtcaaa gacaacaaca agaaggattc ggcctgacct gggccgacag
     3481 gggcaacaag gaaggccctc gccgctatgt gcaggagtag gtgagccatc gggctcgcgg
     3541 catgctcaga tcggcgaggg tcttgtccat cgggcggggg cgacagtcgc caccccgcgg
     3601 atggaacaag aagcgggcgt ccgtaaatgg cc
//
LOCUS       DASVDU010000190        14123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_222355, whole genome shotgun sequence.
ACCESSION   DASVDU010000190 DASVDU010000000
VERSION     DASVDU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14123
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222355"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VF226_12285"
     CDS             <1..711
                     /locus_tag="VF226_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7074813.1"
                     /translation="VRATAVLKPVLQEIASDIATHGCPDRGGDVAVRWSCDDQGRCVI
                     ELSNGNVAETSGIDGCASAGAVQMAFNRPGDGMISCETSEQGARIIVPARYVADWKQS
                     DVRSAPPAVAPADPLEGRSVLVVEDQLIIALDLEMLLKERGAVAVQLSGSVNDALKSI
                     EAERPDIAILDVNLGSTTSFPVARELQRLGIPFIFATGYGNEVEFPPELKSVPLVAKP
                     YCMKKISEALLSTCKAHA"
     gene            complement(1071..1760)
                     /locus_tag="VF226_12290"
     CDS             complement(1071..1760)
                     /locus_tag="VF226_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX7074814.1"
                     /translation="MAGSLALGLAIISPPDLSKAAAPDMPSLSISGRAHVIDGDTLVI
                     DGTRVRLEGIDAPESAQRCQTPTGNGWNCGSAAAQALRGMTAHKEVRCEGSEHDTYGR
                     LIAICRVGALELNKELVRRGLAWAFVRYSQRLTSAEAEARRLKIGIWQGENQPAWDYR
                     ADRWSQAQAQASAPEGCVIKGNISRNGRIYHMPWSPYYDSVQINPSRGERWFCSEAEA
                     LASGWRPAYTN"
     gene            complement(1936..3120)
                     /locus_tag="VF226_12295"
     CDS             complement(1936..3120)
                     /locus_tag="VF226_12295"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEX7074815.1"
                     /translation="MNSELQGRTPTIRHGYVIAAGLALGVAVSNGLGRFSYALILPAM
                     RADLGWNYTQAGALNTTNAIGYLIGALVALNIVRKFGASRLFIGALWITATAIAAMAL
                     TADFNTMIGLRLLAGISGAVVFISGSVLAASLYPRDPTRAAGAIAIYFAGGGVGLLLP
                     GLALPPLFAIGGDGAWPWAWLGMGLFSGAISLVIATAARQVTAPPPHAAPHPWPKRPL
                     LATFASYGCYALGYFAYMTFIVAWMREHGASNTEVAAMWIVLGVATIISPRGWTVPIA
                     RWQGGRPLAGMLLAIGIGALLPLLGASPLIMLTSAALFGQFFMPPAAVTALVKKALPP
                     VVWGEAVAAFTLLFSALQLIGPVLTGAVADQTGSLAWGLGVSGTFLILGAGVALLQPE
                     PK"
     gene            3240..4277
                     /locus_tag="VF226_12300"
     CDS             3240..4277
                     /locus_tag="VF226_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX7074816.1"
                     /translation="MSEPGSIHSAAARIGVLRAARDWLERDGRVALATVIDTWGSAPV
                     PVGGQLAIAADGSFEGSVSGGCIEGEVITEAEEVLSDGRLRVLDFGVADETAWRVGLP
                     CGGQVRVLVERLSKDDGLGYLERALHADQRREALLVRTHLPDGRRELFDKATPDLPPD
                     VSSRLRSGESKLLKTPDGEVFLHAMLPPARIVVIGATHIAQVLCELARMTGYEVIVVD
                     PRTAFASPERFRGVMLHAEWPQDVLPRIGVDPYTAVVVVAHVSHIDDEALKFALKSDC
                     LYIGALGSSRNHAKRRQRLLEAGVSEEAFARIKNPIGLDIGAQTPAEIAVSIMAEVIL
                     AVRGSKAERKG"
     gene            4285..4917
                     /locus_tag="VF226_12305"
     CDS             4285..4917
                     /locus_tag="VF226_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7074817.1"
                     /translation="MAEASRARVAAVLLAAGSSTRFSGGNKLLAEVAGRPLIAWTAAA
                     FLASRADQVIVVTGPEPHAIASALSGMPVRFVENPDHLSGMGGSIAVGVAALSGDSSG
                     VLICPGDMPGVTAELIDRLIAVFEEKGCDRIVRPVLPDGRPGHPVLWPRRFFPRLAQL
                     TGPEGGRVLLRELAGDIDLLPCGDPAAALDIDTAEDLERYRLAKRDLHDR"
     gene            4998..6446
                     /locus_tag="VF226_12310"
     CDS             4998..6446
                     /locus_tag="VF226_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX7074818.1"
                     /translation="MTSGQVETQTAASRPRFGLFGFAVPIWRIAVVALVLLLAAAIAI
                     SPAPAGMPQHSMVALGLVAMTVVLWATLAIPPSAAAIMFTILVLATRAAPPLAAVSGF
                     LSSSLWLVFGGLLIGAAAERTGFGRFIARRFLGRFRSSYSALVLGVIAGTTLLTFLVP
                     SNMGRLAITVPVVLALAKDAGYRLGSNGHIGLVLIAVVGNFGVGQGVLPANLLNIMIV
                     GAGETLYGIDMTYMHYFLLCAPVLGIVKAFLVWRLLIRMHPAPTPCSDEDGSKADQLS
                     PEAKRVGILLALAILLWATDFWHGFKPGWVALLVGVLCITPGIGVLPVREIVNPHRLL
                     IIIWVGTVLSLASVMTESGASTLISSALARLAGVEGQGPAYGYFAMAYLSSLISVLAT
                     FGGAVPIMATVAAEISNATGLPLETAVMAIAPGMSAMFFPYMASPFVVGLAMGHVSHR
                     AALPFMVTLALVTCAIIIPLNAFWWWLLEFLP"
     gene            6629..7159
                     /locus_tag="VF226_12315"
     CDS             6629..7159
                     /locus_tag="VF226_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HEX7074819.1"
                     /translation="MKLFARALGLSILLSLATPALALEYPIGEPQKMHGMEIAAVYLQ
                     PVEMEPEGMMRSAKESDIHLEADIQATADNANGFAEGDWLPHLSIDYKLTKKSSGEEI
                     SGHMMPMVANDGPHYGDNVKLLGPGAYKLELKVSSKSEHFGRHIDKETGVAPWPEDFT
                     ASFEFVYAGTGKKGGY"
     gene            7161..7505
                     /locus_tag="VF226_12320"
     CDS             7161..7505
                     /locus_tag="VF226_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX7074820.1"
                     /translation="MRRIAAFLLAFPFLASVAAWAGDPVFAIEFENGEIRPAEIVVPA
                     NSRFKLELRNVGTSPIEFESLELRKEKVIGPGVTTFIVFNRLSPGEYSFFDDFHPDTP
                     PARLVVVEGKTE"
     gene            7512..8360
                     /locus_tag="VF226_12325"
     CDS             7512..8360
                     /locus_tag="VF226_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX7074821.1"
                     /translation="MTGAFWQVVFIIWRESVEALLVVGILNAWLTRRGGGGGVMKGRR
                     FLWGGAIAGLAFAVVLGWALLNLADMLEGEALEWFQMAMVFAAAGLIVHMVHWMHVSG
                     RTMKATLEGGAERALAAANWWGLFLLAALAVAREGSETVVFLYGVLAGSGGSASAPFV
                     VGAVAGFVAAVITYALIQLGARRLSWSSFFRTSEIVLLLLAAALFIAGTDKAISLGLL
                     PVLSGQLWDTSFILDDGRGWGGFIAALTGYRARPDLIWLIAYSSYWLAVFFLFRPSPV
                     ARPDAA"
     gene            8357..9721
                     /locus_tag="VF226_12330"
     CDS             8357..9721
                     /locus_tag="VF226_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX7074822.1"
                     /translation="MSALAHFGDFLARNQERVRRVQWCVVGLYAFLLVVPVFLPLPGR
                     LDFIWSNLTRFAQFVFWGIWWPGVILSMFLVGRAWCGFLCPEGFLSEEASKRGLGRAL
                     PRWITWPYWPVIAFALTTIYGQVASVYQYPGPALVVLGGSTGAAIIVGLLFGRRKRVW
                     CRYLCPVTTVFAVISKLAPFHFRVDGAAWAASQARGEKPKPVNCAPLVPLRTMQGAAD
                     CHMCGRCSGFRGAIRLSLRSPAHEIVHVAGASPKPAETVLIIFGLMGLSVGAFHWSVS
                     PIFIAIKQQLAAVLVEAGWLWPLEATAPWWLMTNYPSHNDVFNLLDGAVIIAFMLAVT
                     LACGIFISSFVALATVCLGKFHTRRFHHLVQTLIPVAGVGVFIGLSALTVTQLRMDGI
                     TIPYVDEVRAGLVLAASLFSAVLAWKVARLYTASALRRAASLAAMIPVLIVADAGWWL
                     FLSA"
     gene            complement(9731..12745)
                     /gene="polA"
                     /locus_tag="VF226_12335"
     CDS             complement(9731..12745)
                     /gene="polA"
                     /locus_tag="VF226_12335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7074823.1"
                     /translation="MTLETAIEIARRTAECTVPEGEPVPIVKGSHVYLIDGSGYIFRA
                     FHALPPLTRPSDGLPVGAVYGFCSMLWKLLGDSKISDRPTHIAVVFDTARDTFRTAIY
                     EAYKTNRPPPPEELVPQFGLIRRAVKAFNVACIEQDGYEADDLIATYACDAIRKGGDV
                     TIVSSDKDLMQLIQPGVVMLDTMKNKRIGREEVLERFGVEPELVVDVQALSGDATDNV
                     PGVPGIGVKTAAELIREYGNLDALLERAGEIKQPKRRERLIEFAEQARISRELVRLKC
                     DVPLKAPIERLGVQEPDTDALLSFLREMEFNTLTRRIAEKLGVELPPMPERPAAKRPA
                     AKTASGSPAPQRKPDGGAAAAVAGAGTPAAVVAFAEEASLTPAIDRSKYETVTTLERL
                     RHWIAEARNVGRFSLDTETTSRDPMQADLVGFSMAIAPGQACYVPLAHRPASDAFDFG
                     DGNRLEQLPLQTALDAMKPLLEDPSILKIGHNIKYDWLLLSRYGIEIQAFDDTLLMSY
                     ALDGGRGNHGMDELAERHLRHATIRFEKVMEHAPGPKRNGKTFANVPIDKATEYAAED
                     ADVTLRLWTRFKPRLVAERMCTVYETLERPLVPVIAGMERAGIRVDRNVLSRLSGNFA
                     QTIARLEEEVYELVGHKFNLGSPRQLGELLFERLGLPGGKRTKSGQWETRANLLDDLA
                     QNEELPEHARKLVNTMLEWRQLAKLKSTYTDALPTYIHQDTGRIHTCYSLASTTTGRL
                     ASSDPNLQNIPVRTKEGRAIRTAFVAEPGMLLVSADYSQIELRVLAHIADIPALKQAF
                     ADGLDIHAMTASEMFGVPVKDMPAETRRRAKAINFGIIYGISAFGLANQLGISREEAG
                     SYIKTYFARFPGIRDYMERTKAFAHENGYVTTIFGRRVHYPEINTRNPSMRGFLERAA
                     INAPIQGSAADIIRRAMIRMPEALRQAGLSSARMLLQVHDELVFEAKEAEVPKLIETV
                     KHVMEKSPEPAVALSVPLKVDAKAADNWEAAH"
     gene            12856..13182
                     /locus_tag="VF226_12340"
     CDS             12856..13182
                     /locus_tag="VF226_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074824.1"
                     /translation="MPGHTPGHVPLWREDHRALIAGGGFIVTTGRDSAYAVAFQRADL
                     HCRPSVSLKMGIIAPLRNLAARLEAERVTIDHGQPTDDTRLASGASMTLPTRSTRLPS
                     RPMVTR"
     gene            13316..13392
                     /locus_tag="VF226_12345"
     tRNA            13316..13392
                     /locus_tag="VF226_12345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13350..13352,aa:Arg,seq:ccg)
     gene            13827..>14123
                     /locus_tag="VF226_12350"
     CDS             13827..>14123
                     /locus_tag="VF226_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX7074825.1"
                     /translation="MATNVVNLDALIRRADMAEPGEAGSDISSLSLTGLAPKGLLYPA
                     LRKPDFQRETANWSPEQVADLVITFANGDLIPAVILWRSGQYVFVIDGAHRLSAL"
BASE COUNT         2293 a         4410 c         4609 g         2811 t
ORIGIN      
        1 gtgcgcgcaa ccgccgtatt gaagccggtg ctgcaagaga ttgcctccga catcgcgaca
       61 catggctgtc cggaccgggg tggggacgtc gcggtccgct ggtcttgtga tgatcagggc
      121 cgttgcgtca tcgagctgag caacgggaac gtcgcggaga ccagtggcat agatggctgt
      181 gcgtcggcag gggcagttca aatggcgttc aaccggccgg gagatggcat gatcagttgc
      241 gaaacgtcag agcaaggtgc tcgcatcatc gtccccgcgc ggtatgttgc cgactggaag
      301 cagtccgatg tgcgctccgc gcctccggcc gtggctcccg ccgatccgct ggaaggccgc
      361 tcggtcctgg tggtggagga tcagcttatc attgcgctcg atctggagat gctgctcaag
      421 gagcggggtg ccgtcgcagt gcagctcagc gggtccgtga acgacgctct caagtcgatc
      481 gaagccgagc gcccggacat cgccattctc gacgtgaatc tcgggagtac cacgtctttc
      541 cccgtggcac gtgagctgca gcggctgggg attccgttca tttttgcaac tggttacggc
      601 aacgaggtgg aatttccgcc cgagctgaaa tcggttccct tggtcgccaa gccctactgc
      661 atgaaaaaga tcagcgaagc attgctgtcg acctgtaagg cgcacgcctg agcgtccgac
      721 cggagagcag aaataacggc cagatcgcaa gcgacccggc cctgtgcttt ccggaagact
      781 agaccgggcg gttacttgag gagccccgct tccttgtaga agttctcggc gcccgggcgc
      841 aggggcaccc caatcctgta cttcctagcg ctgattgggg ctgcggcagc gcattaaaaa
      901 tggggccggc gcagtgtcga acggcgcttt tggcgctagc gtactgactg ctgatttcgc
      961 aaagctcgcg gtctcggatc ggagcgagct tcgctagcgt gaatgaagtc gagatattcg
     1021 cttccgagac ggcttcgggc acgcaatttc tcgggcacca ctgttggctt tcagttcgtg
     1081 taagcgggcc gccagcccga agccagcgcc tcggcctccg aacagaacca gcgttctccg
     1141 cgcgagggat tgatctggac cgagtcgtaa tagggactcc agggcatgtg atagatgcgg
     1201 ccgtttcggg aaatattgcc tttgatcaca cagccctccg gggccgacgc ctgtgcctgc
     1261 gcctgcgacc agcgatcggc gcggtagtcc caggccggct ggttctcgcc ttgccagatg
     1321 ccgatcttga gccttcgggc ctctgcctcg gcgctcgtga gcctctgtga atagcggacg
     1381 aaggcccagg ccaaaccacg gcgcaccagc tccttgttga gctccagcgc gccgacgcgg
     1441 caaatcgcga tcaggcggcc gtaggtgtcg tgctcagacc cctcgcagcg aacttcctta
     1501 tgggcggtca tgccgcgcag cgcctgcgct gccgccgaac cgcagttcca gccgttaccg
     1561 gtcggggtct ggcatcgttg cgcgctttcc ggggcatcta ttccctcgag ccgcaccctc
     1621 gtcccgtcga tcacgagcgt gtcgccgtcg atgacatgcg cccgcccgga gatgctcagg
     1681 ctcggcatgt caggcgcagc ggccttcgac aggtccggtg gtgaaatgat ggcgaggccg
     1741 agggccaggg aacctgccaa cgccacggct cccaggtgac ggacgattcg cctgcggtca
     1801 gactcacgca tgggttgagc ctgatgcgat tcgtgtgaca gcgtcgaggt gagattgtgc
     1861 tcaaccttgc cgaaggctta acgaatttcg gcggcggcgg ttcaacttgc gggtgcctga
     1921 tgtcagccgg ttcagctact tcggctctgg ctgcagcagc gcgacgccgg cgcccaagat
     1981 gaggaaggtg cccgaaacgc cgagacccca ggcgaggcta ccggtctgat cggcaacggc
     2041 gccggtcagc acagggccga tgagctgcag ggccgaaaag agcagggtga aagccgctac
     2101 cgcctccccc catacaacgg ggggcagcgc ctttttcacc agggccgtta ccgcggcagg
     2161 cggcatgaag aactgaccga aaagcgccgc cgaggtcagc atgatgagag gtgaggcgcc
     2221 gaggagcggc agcagagcac cgatcccgat tgccaagagc atgccggcca gcggccgccc
     2281 tccttgccag cgcgcgatcg ggacggtcca gccccgcggc gagatgatgg tcgccacccc
     2341 aagcacgatc cacatggcgg ccacttccgt attgctcgct ccgtgctcgc gcatccaggc
     2401 gacgatgaag gtcatgtatg cgaaatagcc gagggcatag catccgtagc tggcgaacgt
     2461 ggcgagcagc ggtctcttgg gccatgggtg cggagcagcg tgcggcggcg gggccgtgac
     2521 ctgccgggca gcggtggcga tgacgaggct gatggcgcct gagaaaagtc ccatgccgag
     2581 ccatgcccag ggccacgcgc catctccccc gatcgcgaaa agcgggggca gcgcgaggcc
     2641 gggcaggagc agcccgacac cgcctccggc gaaatagatc gcgattgcac ctgctgcgcg
     2701 cgtcggatcg cgtgggtaca agctcgcggc gagaacgctg cccgagatga agacgaccgc
     2761 accgctgatg cccgcgagca gccgaaggcc gatcatggtg ttgaagtcgg cggtcaaagc
     2821 catggcagcg atcgcggttg ccgtgatcca gagggcccca atgaacaggc gcgacgcgcc
     2881 gaacttgcgg acgatgttga gggcgacgag cgcgccgatc agatagccga tggcatttgt
     2941 cgtattgaga gctcccgcct gagtgtagtt ccagccgaga tcggcgcgca tggcgggtag
     3001 gatgagggcg taggagaacc ggcccaggcc attggaaacc gccacaccga gggccagccc
     3061 tgcggcgatg acgtagccgt ggcggatcgt gggcgtcctg ccttgcagct cagagttcat
     3121 gagacgatca gctgggccct tccgggcaag aggccgagcg acggagcttg caacgcatcg
     3181 gcactcgggg gcattttctg atgtgtccac ctagcggctg caaacggcgg gagcaagaga
     3241 tgtcggagcc cggatccatt cattcggccg cggccaggat cggcgtgttg cgtgcggcgc
     3301 gcgactggct ggagcgcgac ggccgcgtgg cgctggcgac cgtcatcgat acgtggggct
     3361 cggcgccggt cccggtcggc ggtcagctgg cgatcgccgc cgatggaagc ttcgagggct
     3421 cggtctcggg aggctgcatc gaaggtgagg tgatcacgga ggccgaggag gttttgtccg
     3481 acggccggct gcgggtactc gatttcggtg tcgccgacga gaccgcctgg cgcgtcggcc
     3541 tgccgtgcgg cggccaggtg cgcgtcctcg tcgagcggct gagcaaggac gatggcctcg
     3601 gctacctcga gcgcgctctg cacgccgacc agaggcggga ggcgcttctg gtccgcactc
     3661 atcttcccga cggtcgtcgg gagctgttcg acaaggcgac gccggacctg ccgccggacg
     3721 tatcgagccg cctccgatcc ggcgagagca agctgctgaa gacgcccgat ggcgaggttt
     3781 tcctccacgc catgctgccg ccggcgcgga tcgtggtgat cggcgcgacg cacatcgccc
     3841 aggttctctg cgagctggcg cgaatgacgg gatacgaggt catcgtcgtc gatccgcgaa
     3901 ccgcgttcgc atcgccggag cgtttccgcg gcgtcatgct gcatgccgag tggccacagg
     3961 acgtgctgcc gcggatcggt gtcgatccct atacggcagt ggtcgtcgtg gcccacgtga
     4021 gccacatcga cgacgaggcg ctgaagttcg cgctcaaatc cgattgcctc tacatcggcg
     4081 cactcggcag cagccgtaat catgccaagc gccgtcagcg gctcctcgag gctggggtga
     4141 gcgaggaggc attcgcgcgc atcaagaacc cgatcggcct cgatatcggt gcgcaaacgc
     4201 ctgccgagat tgccgtgtcg atcatggccg aggtcatcct ggccgtgcgc ggcagcaagg
     4261 ccgagcggaa gggctgagtc gccgatggcg gaggccagcc gagcccgcgt ggcagccgtc
     4321 ctcctcgcgg cagggtcttc gacccgcttt tcaggcggga acaagctgct ggccgaggtg
     4381 gcgggacggc cgctgatcgc ctggacggcc gctgcgttcc tcgccagtcg cgccgatcag
     4441 gtgatcgtcg tcacggggcc cgagccgcac gccatagcat cggcattgtc cggcatgcct
     4501 gtccgcttcg tcgagaaccc ggatcatctt tcgggaatgg gcggttcgat tgctgtcggg
     4561 gttgcggcgc tgtccggtga ttcttccggc gtcctaattt gtcccggtga catgccaggc
     4621 gtcacggcgg agctcattga ccgcctgatc gccgtcttcg aggagaaggg ctgcgatcgc
     4681 atcgtccgtc cggtcctgcc cgatgggcgc ccaggccacc cggtgctctg gccgcgccgc
     4741 ttttttcctc gtcttgcgca gttgaccggt cctgaaggtg gcagagtcct gttgcgcgag
     4801 ctcgccggcg acatcgatct cctaccctgc ggcgatccgg ccgcggcact cgacatcgat
     4861 acggctgagg atctggagcg ctatcggctg gccaagcggg atctgcatga cagataagcg
     4921 cggctgcgct tgacgatgtg tccgcaactt tggatggctc caatcagcaa tagaaaggtt
     4981 cggggagcta acgcctgatg acctcgggtc aggtggaaac tcaaacggcg gcatcacgcc
     5041 cgcgcttcgg tctgttcggg tttgcggtcc cgatttggcg gattgccgtg gtcgcgctgg
     5101 tgctgctgct ggcggcagcg attgcgattt cgccagcgcc agccggaatg ccgcagcatt
     5161 ccatggtggc gctcggactg gtcgccatga cggtcgtctt gtgggccacc ctggccatcc
     5221 ctccgtccgc tgcagccata atgttcacca tcctggtgct ggcgacgcgc gccgcccctc
     5281 cgctggcggc agtttcgggt ttcctgtcga gctcgctctg gctggtcttc gggggcctgc
     5341 tgattggcgc cgcggcggag cgcaccgggt tcggccgctt catcgcacgc aggttcctcg
     5401 ggcgctttcg cagctcctat tcagccctgg tgctgggcgt gatcgccggc acgacactgc
     5461 tcacgttcct cgtcccctcg aacatgggcc ggctggcgat caccgtgccc gtcgtgctcg
     5521 cgctggccaa ggacgcaggt taccggctgg gctccaacgg ccatatcggt ctcgtgctca
     5581 tcgcggtggt gggcaacttc ggcgtgggcc agggcgtcct gccggcaaac ctgctcaaca
     5641 tcatgatcgt cggtgccggg gagacgctct acggcatcga catgacgtac atgcactatt
     5701 tcctgctctg cgcgccggtt ctgggcatcg taaaggcatt tctggtgtgg cgcttgctca
     5761 tccggatgca cccggcgcca acgccctgct cggacgagga cggatcgaag gccgatcagc
     5821 tcagcccaga ggcaaagcgt gtcggcattc tcctcgcgtt agccatcctg ctttgggcga
     5881 cggatttctg gcatgggttt aagcccggct gggtggcact cttggtcggc gtcctgtgca
     5941 tcacgccagg catcggcgtg ctgccggtgc gggaaatcgt gaacccgcac cgcctgctca
     6001 tcatcatttg ggtcgggacc gtcctgagcc tcgcttccgt catgacggag tcgggagcga
     6061 gcacgctgat ctcgagcgcg ttggcacggc tcgccggcgt cgaggggcag ggccccgcct
     6121 atggatattt cgcgatggcg tacctgtcgt cgctcatctc cgtgctcgcc actttcggtg
     6181 gagccgtccc catcatggcg accgttgccg ccgaaatcag caacgcgacg ggcctcccgc
     6241 tcgaaaccgc cgtcatggcg atcgccccgg ggatgtcggc gatgtttttc ccttacatgg
     6301 cgtccccgtt cgtggtcggg cttgccatgg gccacgtgag ccatcgcgct gcgctgccct
     6361 tcatggtaac tttggcgctg gtaacgtgcg cgatcatcat cccgctgaat gcattctggt
     6421 ggtggttgct tgaattcctg ccgtaattta ggtgatcacc ggccgatatc tcatggcagc
     6481 gcagcgcaag ccgatgctcc ccaggcggct tgctgcgaca cagaatgccg ccttataagt
     6541 tgactgttga agtcaggatt ccaataccat aaagtcggcc accgacacat gccctcggtg
     6601 gagatcgcct gttcatagag gattcctgat gaagctgttc gcgcgcgctc tcgggctttc
     6661 cattctcctg tcgctggcaa cgcctgccct cgcgctcgag taccccatag gcgaaccgca
     6721 gaagatgcac ggcatggaga tcgccgccgt ctaccttcaa cccgtcgaaa tggagccgga
     6781 gggcatgatg cggtcggcca aggaaagcga catccatctg gaagccgaca ttcaggcgac
     6841 cgctgacaat gcgaacggtt ttgccgaggg cgattggctg ccccatttgt cgattgacta
     6901 caaactcacc aagaaaagta gcggtgagga aatttccggc catatgatgc cgatggtggc
     6961 caacgatggc ccgcattatg gcgataacgt caaactgctc ggtcccggcg catacaagct
     7021 cgaattgaaa gtttcatcga agtctgagca tttcggccgc cacatagata aagaaaccgg
     7081 cgtagcgccc tggccggaag acttcacggc ctccttcgag ttcgtctacg cgggcaccgg
     7141 caagaagggc ggctactgat atgcgccgaa tcgctgcttt tctgctcgct tttccgttcc
     7201 ttgcatcggt tgccgcatgg gcaggcgatc ctgtcttcgc catcgaattc gaaaatggcg
     7261 agattcgccc tgctgaaatc gtcgtgcccg ccaattcccg gttcaagctc gagctgcgca
     7321 atgttggaac aagccctatc gagttcgaga gtctcgaatt gcgaaaagag aaggtgatcg
     7381 gcccgggcgt aaccactttc atcgtcttca atcgcctctc gccgggcgag tacagcttct
     7441 tcgatgactt ccatcccgat acgccgccgg cgcgattggt cgtggtggaa ggcaaaacgg
     7501 aatgaacggt catgaccgga gcattctggc aagttgtttt catcatctgg cgcgagagcg
     7561 tcgaagcgct tctggtcgtc ggcattctga atgcctggct gacccggcgc ggcggcggag
     7621 gcggggtgat gaaaggccgg cgctttcttt gggggggcgc cattgcgggg ctggccttcg
     7681 ccgtggtgct gggttgggcc ctgctcaatc tcgccgacat gctcgagggc gaggcgctcg
     7741 aatggttcca gatggctatg gtctttgctg cggccggtct catcgtgcac atggttcatt
     7801 ggatgcacgt gtcgggccgg accatgaagg ccacgctcga aggcggtgca gaacgggcgc
     7861 ttgccgcggc aaactggtgg ggactctttc tgctcgccgc actggcggtg gcgcgcgagg
     7921 gcagcgagac ggtcgttttt ctttatggag tgctggccgg cagcggcggc tcggctagtg
     7981 ctccctttgt cgtcggggcg gtcgccggtt tcgtggccgc cgttataacc tacgccctga
     8041 tccagctcgg agcccgccgc ctatcctggt cgagcttttt tcggacgagc gagatcgtgc
     8101 tcctgctcct cgccgccgca ttgttcattg ccggcaccga caaagccatc tctctcggcc
     8161 tcttgccggt cctttccggg cagctgtggg atacgtcatt catcctcgat gacgggcgcg
     8221 gctggggtgg cttcatcgcc gccctgacgg gctaccgcgc ccggccagat ctcatctggc
     8281 tgatcgccta tagcagctac tggctggcgg tattttttct gttccgccca tcgccggttg
     8341 cccgcccgga tgctgcatga gcgcgttggc gcatttcggt gatttccttg cacgcaacca
     8401 ggaaagggtg cggcgggtgc aatggtgtgt ggtggggctc tacgccttcc tgctcgtggt
     8461 gcccgttttc ctgccgctcc ccggaaggct cgacttcatc tggagcaacc tgacccgttt
     8521 cgctcagttc gttttttggg gcatctggtg gccaggcgtc atcctttcga tgttcctggt
     8581 gggccgggcg tggtgtggat ttctgtgtcc ggaaggcttc ttgagcgagg aggcgagcaa
     8641 gcgcgggcta gggcgcgcct tgccgcgctg gatcacgtgg ccgtactggc ccgtaattgc
     8701 attcgcgctc accacgatct acggccaggt tgcgagtgtc taccagtatc ctggtccggc
     8761 gctcgtcgtt ctgggcggtt cgacgggggc cgcgatcatt gtcggcttgc tttttgggcg
     8821 gcgcaaacgt gtctggtgcc gttacctttg tccggtcacc accgtgtttg cggtgatttc
     8881 caagctcgcg ccgttccatt tcagggtcga cggcgccgcc tgggccgcct cacaggcgcg
     8941 cggtgaaaag ccgaagcccg tgaattgtgc gccgctggtg ccgctccgca cgatgcaagg
     9001 cgcagccgac tgccacatgt gcgggcgctg ctcggggttt cgcggagcga tccgcttgtc
     9061 tctgcgctcc ccggcccacg aaatcgtcca cgtggccggt gcgagcccca agcccgccga
     9121 aacggtcctg ataattttcg gcctgatggg cttgagcgtc ggtgccttcc actggtcggt
     9181 tagtccgatt ttcattgcca tcaagcagca gcttgctgcc gtcctcgtcg aagccggctg
     9241 gctctggccc ttggaggcga cggcgccgtg gtggctgatg accaactatc catcgcacaa
     9301 cgatgtcttc aatttgctcg atggggcggt gatcatcgcg ttcatgctgg cagtcactct
     9361 cgcctgcggg attttcattt catcgttcgt ggcgctcgcc accgtctgcc tcggcaagtt
     9421 ccatacacgc cgtttccatc atctggtgca gaccctcatt ccagtggccg gagtgggcgt
     9481 cttcatcggt ttatcggcac tcacggtcac gcagctgcgc atggacggaa tcaccattcc
     9541 ttacgtcgat gaggtacgcg ccggcttggt gctggctgcg agcctgttct cagccgtgct
     9601 cgcctggaag gttgcccggc tctataccgc atccgcactt cggcgtgccg cttcgctcgc
     9661 ggcaatgatc cctgtgctca tcgttgccga tgccggctgg tggctgttcc tcagcgcttg
     9721 aatggcggct tcaatgcgcc gcctcccagt tatccgccgc cttcgcatcc actttcagcg
     9781 gcacggacag cgcaacggcg ggctccgggc tcttctccat gacgtgcttc acggtctcga
     9841 tcagcttggg gacctcggcc tccttcgcct cgaacacgag ctcgtcgtgc acctgcagca
     9901 gcatgcgtgc cgacgacagc cctgcctgcc gcagcgcctc gggcatgcgg atcatggcgc
     9961 gacggatgat gtcggccgcc gagccctgga tcggcgcgtt gatggccgcg cgctcgagaa
    10021 agccgcgcat ggacggattg cgcgtgttga tctcgggata atgcacccgc cggccgaaga
    10081 tggtcgtgac gtaaccgttc tcatgcgcaa aagctttggt gcgctccatg taatcgcgga
    10141 tgccgggaaa gcgggcgaag tacgtcttga tgtagcttcc tgcctcctcc cgcgagatgc
    10201 cgagctggtt cgccagtccg aacgcggaga tgccgtagat gatgccgaag ttgatggctt
    10261 tggcccggcg cctggtctcg gccggcatgt ccttcaccgg cacgccgaac atctcggacg
    10321 ccgtcatggc gtgaatgtcg agcccgtcgg cgaatgcctg cttcaaggcc gggatgtccg
    10381 cgatgtgggc aagcacgcgc aactcgatct ggctgtagtc ggcagagacc agcagcattc
    10441 ccggctccgc cacgaaggcc gtgcggatgg cgcgcccttc cttggtgcgc acaggaatgt
    10501 tctgcaggtt gggatcgctc gaggcgagcc gtcccgtcgt cgtcgacgcc agcgagtagc
    10561 aggtgtgaat gcgcccggta tcctgatgga tgtaggtcgg cagagcatcc gtataagtcg
    10621 atttcagctt ggcgagctgg cgccattcca gcatcgtgtt gacgagcttg cgcgcatgct
    10681 ccggcagctc ctcgttttgc gcgaggtcgt cgagcaggtt ggcgcgcgtc tcccattgac
    10741 ccgatttggt gcgcttgccg cccgggagcc cgagccgttc gaacagcagc tcgcccagct
    10801 ggcgcggcga gcccaggttg aatttgtgcc cgacgagctc gtagacctcc tcctctagcc
    10861 gcgcgatggt ctgggcgaag ttgccgctca agcgcgagag cacgttgcga tcgacgcgga
    10921 tgccagcgcg ctccatgcct gcaatcacag gcaccagcgg gcgctccagc gtctcgtaaa
    10981 ccgtgcacat gcgctctgcg acgaggcgcg gcttgaagcg ggtccacagc cgcagtgtga
    11041 cgtcggcatc ctcggcggca tattccgtcg ccttgtcgat cggcacgttg gcgaacgttt
    11101 tgccgttccg cttcggcccc ggcgcatgct ccatcacctt ctcgaagcgg atggtcgcgt
    11161 gcctgagatg ccgctcggcc agctcgtcca tcccgtggtt gccccgcccg ccgtcaagcg
    11221 cataggacat gagcagcgtg tcgtcgaagg cctgaatctc gatgccgtag cggctgagca
    11281 ggagccagtc gtacttgatg ttgtggccga tcttgaggat cgacgggtcc tcgagcagcg
    11341 gcttcatcgc atccagcgcc gtctgcagcg gcagctgctc cagcctattg ccgtcgccga
    11401 agtcgaaggc gtcacttgcc ggcctgtggg cgagcggcac atagcaggcc tgccccggcg
    11461 cgatcgccat ggagaagccg acgagatccg cctgcatcgg gtcgcgcgac gtggtttcgg
    11521 tatcgaggct gaaccggccc acattgcgcg cctctgcgat ccagtggcga aggcgctcga
    11581 gcgtggtgac cgtctcgtat ttcgagcggt cgatcgcggg cgtgagggag gcctcctcgg
    11641 cgaaggccac cacggctgct ggagttcccg ctccggcaac tgcagccgca gcgccgccgt
    11701 caggcttcct ttggggcgcg gggctcccgg atgccgtctt cgctgccggc cgctttgccg
    11761 cgggcctttc cggcatcggc ggcagctcga cgccgagctt ctccgcgatc cgccgggtca
    11821 gcgtgttgaa ctccatctcg cgcagaaagc tgagcagggc gtcggtgtcc ggctcctgca
    11881 ccccgagccg ctcgatcggc gccttcagag gcacatcaca cttgaggcgc acgagctcgc
    11941 gcgagatgcg cgcctgctcg gcgaactcga tcagccgctc gcgccgcttc ggctgcttga
    12001 tctcgcctgc gcgttccagc agcgcgtcga gattgccgta ctcgcggatc agctctgcgg
    12061 ccgtcttgac gccgataccg ggcacgccgg gcacgttgtc ggtggcatcg cccgacagcg
    12121 cttgcacgtc gaccaccagc tccggctcta cgccgaagcg ttccaacacc tcctcgcgcc
    12181 cgatgcgctt gttcttcatc gtgtccagca tgacgacgcc gggctggatg agctgcatca
    12241 gatccttgtc cgaggacacg atggtgacgt cgccgccctt gcggatggca tcgcacgcat
    12301 atgtcgcgat caggtcgtcg gcctcgtagc cgtcctgctc gatgcaggcg acattgaagg
    12361 ccttgaccgc ccgccggatc agcccgaact ggggaaccag ctcctccggc ggcggtggcc
    12421 ggttcgtctt gtaggcctcg tagatggccg tccggaaggt gtcgcgcgcc gtatcgaaga
    12481 cgaccgcgat gtgtgtcggc cggtcggaga tcttgctgtc gccgagcagc ttccagagca
    12541 tgctgcagaa gccgtagacg gcgccgaccg gcagtccgtc ggagggccgt gtcaggggcg
    12601 gcagcgcgtg aaaggcgcgg aagatataac ccgagccgtc gatcagatag acgtgactgc
    12661 ccttgacgat gggcacgggc tctccctccg gcacggtgca ttcggccgtc cggcgggcga
    12721 tttctatggc ggtttcgagc gtcatgggcg gcactatacg cggtcaggtc tcccgggtca
    12781 gcagcctggc gcggaaccgt cgtcgcgggc gtcggcttgg gctggaagtt cgggattttc
    12841 ttcggtggat tgaagatgcc gggccacacg cctggccatg tgccgctctg gcgggaagac
    12901 catcgcgcgc tcattgccgg cggtggcttc atcgtcacga cggggcgaga ttcggcctat
    12961 gctgtcgcgt tccagcgcgc agacctgcac tgtcggccgt ctgtttcact caaaatgggt
    13021 ataatcgcgc cactccgcaa cctggctgcc aggctcgagg ccgagcgggt caccatcgac
    13081 cacggccaac caaccgatga tacaaggctt gcgtcaggtg cttccatgac cttgccgaca
    13141 cggtcgacca ggttgccatc ccggccgatg gtcactaggt gacctatgca acgacgctga
    13201 cgacagcgcc gcccacgtac atgggattga ggggcaggcc ggttcggcag gtgcttcgac
    13261 caacccatcc cgcgattgcg ggatcgtgcc ggatccgtta ttgtcccggc ctcacgcacc
    13321 catagctcag ctggatagag cgctgccctc cgaaggcaga ggtcagaggt tcaaatcctc
    13381 ttgggtgcgc cagacgcctc ccgccacccc tttgaatctt ctcccttttt tgccacctga
    13441 gtcagggggg tggttcctcg tcggttgctg gcacacagcc cgaaattgcc cggaaatgcc
    13501 tgtcaagtcc cgacgcaagt cccgacgcgg atcagtcgga ccggacgggt ggtcggcgac
    13561 cattcgacta tatgagcgac ttaccttagc tacgagtatt agatgtggat tgcgccatct
    13621 agcgcggaac caacttgctt tgtgtcgata caatccgcgc taaagtgagc ggcgtcgggt
    13681 gcttgatggc gcgacagacc ctgcacgccg ctaatcctct gcttgccaga ggtcacaaga
    13741 cgggccgccg aatcaaagct gggctgtgct agcttggtgg cgcagctcgt accgacaact
    13801 gacttgttgc ctaaggggag ggagggatgg caacgaatgt tgttaatctt gacgcactga
    13861 tccggcgggc tgatatggcg gagccgggag aagcagggtc agacatttct tcactgtcgc
    13921 tcactggact tgctccgaag ggattgctgt atccggcatt acgtaagcca gatttccaac
    13981 gtgaaaccgc taactggtcc cccgagcaag ttgcagattt ggtcatcact tttgcgaacg
    14041 gcgatctgat acctgccgtc atactttggc ggtcggggca atacgtattc gttatcgacg
    14101 gtgcgcacag attaagtgcg ctt
//
LOCUS       DASVDU010000191         7194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1120645, whole genome shotgun sequence.
ACCESSION   DASVDU010000191 DASVDU010000000
VERSION     DASVDU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120645"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1813)
                     /locus_tag="VF226_12355"
     CDS             complement(<1..1813)
                     /locus_tag="VF226_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7074826.1"
                     /translation="MTAVLMWPTVAAGQSAAPGTTVELSPIVIEGGAPQPGSVTGAES
                     LGEARGDGEEGSAVDRATRTLDAIAGGTAVVASQEMPPQAGTSLSEALAFVPGVVVQD
                     FFGSNDQPRLQIRGSGLQQNPSERGTLVLQDGLPINRADGSYIVGLADPKSAAFMEVY
                     RGYTANRLGATVLGGAINFVSPTGITAPGADVAAEFGSFGQFTTNAQAGARQGDLDAH
                     ASLSYTERDGFRQHNESERANFNMNVGARVNENISTRLFVGYTDLAFDVVGPLSRDLL
                     EQNPKQICSGPPNCKGPNVGRDDPRREAEQFRIGSRTTARFGANRFDFGLGYTYTDDS
                     FTFPIAGGVRDTVGGDLTTVLRYAYAPDPSRALPLFEVTGQYVVGSADRRYFINDKGK
                     KGPLFGDNELDSSTLSVNAGFNIPLTERLTVSPALAYSYATRENKDVYKGATRPYLAF
                     PMPQPFGETPFKDTSYDHSYSAWTPSLALSYEIARGQSVFAALSRSFEPPTHDDLIAT
                     INGSPNSSAGAPALPPTETPPPPRDAFATPDLDAQTATTLEGGWKGRTDRLAWSAIAY
                     YSWVKDELLNLRDETAVSLGAVNADKTTHFGVELGAAF"
     gene            2079..2768
                     /locus_tag="VF226_12360"
     CDS             2079..2768
                     /locus_tag="VF226_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7074827.1"
                     /translation="MTMDCRDVLKLRRAPFLAGLGAEELDAFASSLEVATLSAGQTLF
                     SQGDPATAFYFVLDGWVALFRDGPEGERTAIHLVGPGESFAEALLAHEARYPVTAEAA
                     ADLRVAVISIARFRSLITQSPELALSIISAVLVKLRALVSRIEQDQEWPPQRRIAAFL
                     YRTCMANGGACQFELPVEQRYIAARLSMTPATLSRALSDLAAIGVEARRRKVVITDVG
                     RLARFVNQDQP"
     gene            complement(3003..4268)
                     /locus_tag="VF226_12365"
     CDS             complement(3003..4268)
                     /locus_tag="VF226_12365"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX7074828.1"
                     /translation="MCTRSPDLLDSLAAALPGLREPLSPDADERRTFAERRRLRINEL
                     RDHVECSIVGTCLTHTDLLNIAKRCRVQVSPDVTAYDLHAHFVEAVRKGGPLARAMQK
                     LLDRRHEGILRIVGRARSREELEQIWRREYDAGRIAGAYWAFQTYSHIPQDLAARIFG
                     EVHMLSHVLGRTIHAAAERASELAARVADLEAKIARDAERHRQALQERDAQIAELRSR
                     LIASVPSPLTQQRPLLERRGSKRDRAVLAARERAKLAEARIDELLRENAALRERALRA
                     ERAAASVRAQEECPGALACRLDLPREEKLKVLYLGGRKGSIEQLRAIAEGASAEFYHH
                     DGGVEQAFQRISDMVERCHVVFCPVDCINHRACLLAKHQCQRMQKAFVPLRSAGGATF
                     KQALSEITRATRHEPAQFIRGSSEPTDVE"
     gene            4551..4976
                     /locus_tag="VF226_12370"
     CDS             4551..4976
                     /locus_tag="VF226_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074829.1"
                     /translation="MLKSWRWIASAATATAFMGATPALAEPKIHPLGIELNTIEPTDG
                     KCRLTFVMSNPADAPVDSLKADTAVFGPDGVIKRRLVIEFGPLKPKKTSIRAFDLDAT
                     CDSLGSLLVNDIIACAPETLGDCLARLELSSRTSVGLFK"
     gene            5003..5668
                     /locus_tag="VF226_12375"
     CDS             5003..5668
                     /locus_tag="VF226_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7074830.1"
                     /translation="MTPIGLFMQAGPVGKAVILILLVMSIWCWVLILEGWIAVVRLRR
                     AVRRLRAGAGNSVSPLLIEVIKAGDEARRIDVPMETAGETRQRIGEAMRRAAQVLLGR
                     LDSGLPSLAVISSVAPFIGLFGTVWGIMTSFASIADANDTSLAVVAPGIAEALAATAI
                     GLAAAIPASFGYNRLSSSMGRVAQMLDHLIEDQAVALMARPAPAHELHRDREPRRGHA
                     VAQ"
     gene            5652..6119
                     /locus_tag="VF226_12380"
     CDS             5652..6119
                     /locus_tag="VF226_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7074831.1"
                     /translation="MPSRNNSGNALYQPMPEINVTPLVDVMLVLLIVFMITAPMLTAG
                     LKVNLPQAKSAEPLDPKQPVVITVTKEGDVTIGDQTVAREDIASEVRRLLGEEDRPVH
                     VRGDREVPYGEIVAVMDDLAANGIVKIALLATGRSTRNGESMELRAQAGPQAP"
     gene            6198..7019
                     /locus_tag="VF226_12385"
     CDS             6198..7019
                     /locus_tag="VF226_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7074832.1"
                     /translation="MAVLLVTALYGAMLVRLPAPTPAGVSLQPAEVMILPPGDPEQEI
                     AAVGSPDSIAAQASDGLQSASVAELSELEPTEELTPAKSQASEVRTVEPVEASAIAPM
                     LQAEPPLEAVESAVAIAPKELDPAKMSDRTPVTDAPVRREVVKTEVVEKGKPKDDTKK
                     EPAKKKSASSPAQRGHAGSGGGQRNASAQSIAAYAAHVRSVLVSRTQRIRNLRSSGRV
                     GLTFTINADGRLASLSLRSSGRAEIDQAVRASVRGAIFPPPPNGRFRGSITITVR"
BASE COUNT         1270 a         2274 c         2352 g         1298 t
ORIGIN      
        1 ggaaggccgc cccgagctcg acgccaaaat gcgtcgtctt gtcggcattg accgcgccga
       61 gcgagacggc cgtctcgtca cgcagattga gcagctcgtc tttcacccag gagtaatagg
      121 cgatggcgga ccaggcgagc ctgtccgtcc ggcccttcca gccaccttcc aatgtggtcg
      181 cggtctgcgc gtcgagatcg ggcgtcgcga acgcatcccg gggcggaggc ggtgtctccg
      241 tcggaggcag agcgggagcg ccggcgctgc tgttcggcga gccgtttatc gtcgcgatta
      301 gatcgtcatg cgtcggcggc tcgaagctgc ggctcaacgc agcaaagacg gactgaccgc
      361 gcgcgatctc gtagctcagc gcaaggcttg gggtccatgc cgaataggag tggtcgtagc
      421 tcgtgtcctt gaagggagtc tcgccgaacg gctgcggcat tggaaatgcc aggtaaggcc
      481 gtgtggcgcc cttgtagacg tccttgttct cccgcgtcgc gtaggaatat gcgagagccg
      541 gcgagaccgt cagccgttcc gtgagcggga tgttgaagcc cgcgttgacc gacaacgtgc
      601 tcgagtcgag ctcgttgtcg ccgaacagcg gccccttctt gcccttgtcg ttgatgaaat
      661 agcgccggtc cgcgctgccg acgacgtact ggcctgtcac ctcgaacagt ggcaatgccc
      721 gactggggtc tggcgcatag gcatagcgga ggacagtggt gaggtcaccg ccaacagtgt
      781 cacgtacgcc acccgcgatc gggaacgtga agctgtcgtc ggtataggtg taacccaagc
      841 cgaaatcgaa gcggttggcg ccgaagcgcg ccgtcgtccg cgagccgatg cggaactgct
      901 cggcctcgcg ccgcggatcg tcacggccga cgttcgggcc cttgcagttc ggcgggccgg
      961 agcagatttg cttcgggttc tgttcaagga gatcccgcga cagcgggccg acgacgtcga
     1021 atgcgaggtc ggtgtagcca acgaacaggc gcgtgctgat gttctcattc acccgtgcgc
     1081 cgacgttcat gttgaagttg gcgcgctctg actcattgtg ctgacgaaat ccgtcccgct
     1141 ccgtgtaact gaggctggca tgggcatcga gatcgccttg ccgcgcgcca gcctgtgcgt
     1201 tcgtcgtgaa ctggccgaag ctgccgaact cggcagccac gtccgcgccc ggagcggtga
     1261 tcccggtcgg cgagacgaag ttgatagcgc cgccgaggac ggtggcgcca agccggttgg
     1321 cggtgtagcc gcgatacacc tccatgaagg cggcactctt cggatcggca aggccaacga
     1381 tgtacgagcc atctgctcgg ttgatgggca ggccatcctg cagcaccagg gtgccacgct
     1441 cggacggatt ctgctgcagg ccggagccgc ggatctgcaa tcgcggctgg tcgttgctac
     1501 cgaagaaatc ctgcacgacg acacccggca cgaaggccag cgcttcggac agcgaggtgc
     1561 cggcctgcgg cggcatttcc tggctggcga ccaccgccgt tccgcctgcg atcgcatcga
     1621 gcgtccgcgt cgccctgtcg acggccgagc cctcctcacc atctccgcga gcctcgccca
     1681 gcgattccgc accggtgacg ctccccggtt gcggcgcccc gccctcgatg acgatgggag
     1741 acaattcgac cgtggtgcct ggcgcagccg attggcctgc cgccactgtc ggccacatca
     1801 aaacggctgt catcgcgacg agactgccat tccgcttcag aagctccgcc gtccgccgcc
     1861 cccgcgaatc ggcaacatgt cgacaatcgg caggcacatg cctttgacag accatcatct
     1921 tcgctccccc gttggtctat tcttgactag aataatcgag atatgatgcg agggacttgc
     1981 ctgggatcaa ctcagggtcc gtggcgggaa cggccgattg acgcatatca ggctgtgacc
     2041 gccacagggg cacgcacgag gagcatggcc acgccgtaat gaccatggac tgtcgcgatg
     2101 ttctgaaact caggcgtgct ccgttcctgg cgggattggg cgcggaggag ctcgacgcgt
     2161 tcgcctcgag cctcgaggtg gcgacgctct ccgctggaca gaccctattc agccagggcg
     2221 accccgcgac cgcgttctac ttcgtcctgg atggatgggt tgcgctattc cgcgacggcc
     2281 ccgagggcga gcggacggca atccacctcg ttggcccggg ggaatcgttt gcagaggcgc
     2341 tgctggcgca tgaggcgcgc taccccgtca ccgccgaagc ggcggcggac ctgcgtgtgg
     2401 ccgtcatcag catcgcgcgt tttcgatcgc tcatcacgca atcgccggag ctggcgctgt
     2461 cgatcatctc cgccgttctt gtgaaactgc gcgctctcgt ctcacgcatc gagcaggacc
     2521 aggaatggcc gcctcagcgc cggattgcgg cgttccttta cagaacctgc atggccaacg
     2581 gcggcgcctg tcagttcgag ctgcccgtcg agcagcggta tatcgcagcc cggttgtcga
     2641 tgaccccggc gacactgtcg cgtgcactga gcgatttggc tgccatcggt gtggaggcga
     2701 ggcggcgcaa ggtcgtgatc accgacgtag gccgcctggc ccggttcgtg aaccaggacc
     2761 agccgtaagg ccgcctctga tcagatcgtc acctgcgtgg gcatcggcca cggccaccac
     2821 atgccgggca cgtgcagagg cagtcggaaa tgcaggcaaa gccgcatccg cagcattcga
     2881 agcagacctg ttgccctctg ccatgctcgg gcgaaagcgc gtccgcctca ttattattcc
     2941 gcagctgcag ctcaaatccg ccttcaagac cccgctcgac gggaaacgcc gcatggtcgg
     3001 cttcattcga catcggtggg ctcggatgat ccccggataa actgtgccgg ttcatgacgt
     3061 gtcgctcgag tgatctcgct cagggcctgc ttgaatgtcg cgccaccggc ggatcgaagg
     3121 ggcacgaaag ccttctgcat gcgctgacac tgatgtttgg caagcaggca ggcgcggtgg
     3181 ttgatgcagt cgacgggaca gaaaaccacg tgacagcgtt ccaccatatc gctgatgcgc
     3241 tggaaggcct gctcgacgcc gccgtcatgg tggtagaact cggccgaggc gccctccgca
     3301 atggcgcgca gctgctcgat ggaccctttc cgaccaccga gatagagaac cttgagcttc
     3361 tcctcccgcg gcaagtcgag ccggcaggca agggcgcctg ggcattcctc ctgcgcgcgg
     3421 acactcgccg cggcacgctc ggcacgcagc gctcgttcgc ggagcgcggc gttctcgcgt
     3481 agcagctcgt cgatgcgtgc ctcggcaagt ttggcgcgct cgcgcgccgc aaggacggcc
     3541 cggtcgcgtt tcgagccacg cctttccagc aaaggtcgtt gctgagttag cggcgacggc
     3601 acgctcgcga tgagtcgcga ccgaagctcg gcgatttgcg cgtcgcgctc ctgcaacgct
     3661 tggcgatggc gctcggcgtc ccgcgcaatc ttggcctcca gatcggcgac gcgcgctgcc
     3721 agctcggacg cacgctctgc ggcggcatgg atcgtccggc ccaggacatg gctcagcatg
     3781 tgaacttcgc cgaagatgcg ggcggcaaga tcctgcggga tgtgactgta ggtctgaaag
     3841 gcccagtagg cacccgcgat acgcccggcg tcgtactcac gcctccagat ctgctccagc
     3901 tcctctctcg accgggcccg gccgacgatg cggagtatgc cttcatgccg gcgatcgagc
     3961 agcttctgca tggcgcgcgc gagcggcccg cccttgcgga ccgcctccac gaagtgcgcg
     4021 tgaagatcat acgcagtgac gtcggggctg acctggactc ggcagcgctt ggcgatgttc
     4081 agcaggtccg tgtgggtgag gcaggtgccc acgatcgagc actcgacgtg atctcgcagc
     4141 tcgttgatcc tgaggcggcg ccgctccgcg aaagtgcggc gttcatcggc atcgggcgat
     4201 aaaggttcgc ggagccctgg cagcgctgct gcgagactat cgaggagatc tggcgatcgc
     4261 gtgcacatgg gtcgcgtaca tcgggtgagc aaagggcaac gtcggcaagg ccaagccccg
     4321 ccctcctaaa cttgattatt atactccaca atccaggatt gctggaagga acattttaaa
     4381 gaactcgctg agcaggatgt gcatctcgtt gtcgcgggct ggggcggtcg caccggcaag
     4441 gtcgggcagc actattcttg acaatggctg tatagaaatg gcaatgcagt tggaccacgg
     4501 acttgcattg cagccaggct tggaacgggc atctagcggc gggggaattc atgctgaaga
     4561 gctggcgatg gatcgcaagc gccgcgactg cgaccgcttt catgggggca acgccggcgc
     4621 ttgccgagcc caagatccat ccgctcggca tcgagttgaa cacgatcgag ccgacagatg
     4681 gcaaatgccg gctgacgttc gtcatgtcga acccggcgga cgctccggtt gatagcctga
     4741 aagccgacac ggccgttttt ggacccgatg gcgtgatcaa gcgccggctg gttatcgaat
     4801 tcggtccact gaagccgaaa aagacgagta tccgcgcgtt cgatctggac gcgacctgcg
     4861 attccctcgg ctcgttgctc gtgaacgaca tcatcgcctg cgcgccggaa accctgggtg
     4921 actgcctggc gcgcctcgag ctgtcgtcgc ggacctcggt cgggctgttc aagtaggaac
     4981 ggagggcgac ttggagcaca cgatgacgcc gatcggcctg ttcatgcagg cagggccggt
     5041 tggcaaggcg gtcatcctca tactgctcgt catgtcgatc tggtgctggg tgctgatcct
     5101 ggagggctgg atcgccgtcg tccgtttgcg ccgcgcagtc cgtcgactgc gcgctggtgc
     5161 gggaaattcc gtttcgccct tgctgataga ggtgatcaag gccggcgacg aggcccggcg
     5221 catcgatgtg cccatggaga cagccggcga aacccgacag cggatcggtg aagccatgcg
     5281 ccgggcagcc caggtgctgc tcggccgttt ggacagcggc ctgccgagcc tggcggtgat
     5341 ctcgtcggtc gcgcctttca tcggcctgtt cggcaccgtg tggggcatca tgacgagctt
     5401 cgccagcatc gccgatgcca acgatacgag ccttgccgtc gtggcccctg gcatcgccga
     5461 ggccctggca gccacggcga tcgggcttgc ggccgcgata cctgcctcgt tcggctacaa
     5521 tcgcctttcg agctcaatgg gtcgcgtggc ccagatgctc gatcacctga tcgaggatca
     5581 ggccgtcgcc ttgatggcca gacctgcccc cgctcatgag cttcatcgag acagggagcc
     5641 gcgccgtggc catgccgtcg cgcaataact cgggaaacgc gctctatcag ccgatgcccg
     5701 agatcaacgt gacgccgctc gtcgacgtca tgctggtgct gctcatcgtt ttcatgatta
     5761 ccgcaccgat gctgacagcg gggctcaagg tgaatctgcc gcaggcgaaa tcggccgagc
     5821 cgctcgaccc caagcagccg gtcgtcatca cggtgacgaa agaaggcgat gtgacgatag
     5881 gtgatcaaac ggttgcgcgg gaggacatcg cttcggaagt gcgcagactg ctcggcgaag
     5941 aggatcggcc cgttcatgtc cgcggcgacc gcgaggtccc ttatggcgag atcgtcgccg
     6001 tcatggatga tctggcggct aacggcattg tcaagattgc gctgctggcg accgggcgca
     6061 gcacacgcaa cggcgaatcc atggagctgc gtgcgcaggc cgggccgcag gcgccgtgac
     6121 gcgtcgcgat gccgtcgggc gaaacaactc tgagcacttt ccgagctcca agattggtgc
     6181 cgatcggggt tcgtccaatg gcggtcttgc tggtgaccgc gctttatggc gcgatgctgg
     6241 ttcggctgcc tgcgccaaca cccgctggcg tctctctgca gccggcggag gtcatgatcc
     6301 ttccgcctgg agatccggag caggagatcg cggctgtcgg aagtccggac tcgatcgcgg
     6361 cacaggcaag cgatgggctc caatccgcgt ccgttgccga gctttcggag ttagagccca
     6421 cagaggagtt gacacctgca aaatcgcagg cctccgaggt gaggaccgtt gaaccggtcg
     6481 aagcgtcagc tatcgcgccc atgctgcagg ccgagccgcc gctcgaagcc gtggagtcgg
     6541 cggtggcgat cgctcccaag gagctcgatc cggctaagat gagcgatcgc acgccggtga
     6601 ctgacgcgcc ggtgcggcgc gaggtcgtca agaccgaagt ggttgagaag ggaaagccca
     6661 aagacgatac gaagaaagaa ccggcaaaaa agaaatcagc ttcgagcccc gcgcagcggg
     6721 gtcacgccgg ttctggcggt ggccagcgaa acgcgtcagc gcaaagcatc gcagcctatg
     6781 ccgcgcacgt ccggtccgtt ctggtctccc gaacgcagcg aatccgaaat ttgcgctcct
     6841 cggggcgcgt cggccttacc ttcacgatca acgccgatgg ccgcctcgcc tcgttgtcgc
     6901 tgagatcctc cggtcgcgca gagatcgacc aggccgttcg tgcatccgtc cgcggcgcca
     6961 tcttcccgcc cccgccaaat gggcgtttca ggggaagcat cacgattacc gtcaggtgag
     7021 agcttattaa ggcgctgcca tccactggcg cgccgaggct cagctatcgc cacttcttaa
     7081 gctgaaacca cccgcatcgc agacggccgc aaacgcgttt ctgctccagg tgcagtagga
     7141 ggaagcgcct acgtgccaac ctgccagtgc tcctgccggc cggtgagcgt cagg
//
LOCUS       DASVDU010000192         8349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1417245, whole genome shotgun sequence.
ACCESSION   DASVDU010000192 DASVDU010000000
VERSION     DASVDU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417245"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1269
                     /gene="dnaJ"
                     /locus_tag="VF226_12390"
     CDS             136..1269
                     /gene="dnaJ"
                     /locus_tag="VF226_12390"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX7074833.1"
                     /translation="MAKRDYYEVLGVSRNASEQELKSAFRRLAKECHPDHNSGDKSAE
                     QRFKEIAEAYETLKDPQKRAAYDQFGHAAFDGGGRGAGGFGPDFAASMSDIFDDLFGE
                     FMGGRRGRQRSGRERGADLRYNLEITLSEAYTGKTAQIRVPSSVACEKCSGSGAKPGT
                     KPSSCPTCAGFGKVRASQGFFTIERTCPTCQGRGEVIDDPCPACSGSGRVMRERTLSV
                     NIPAGVEDGTRIRLAGEGEAGLRGGPAGDLYIFLSIKPDEFFQRDGADIFCMVPISMC
                     TAALGGQIEVPTVDGSVTRVKIPEGTESGKQFRLKNKGMPVLRSKSYGDMYIQVQVET
                     PKNLTRRQRELLEEFERESHKDTSPESAGFFSRVKEFFEGRGV"
     gene            1500..2663
                     /locus_tag="VF226_12395"
     CDS             1500..2663
                     /locus_tag="VF226_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7074834.1"
                     /translation="MTLSEREAQITAWLAEQKDAMIALLREVVNIDSGTYDKAGVDAV
                     GARFEQFFAEHGLPTRREPNERFGDAIHIRLDDRLTNEKPVLLLGHRDTVFSKGEAER
                     RPFRIEGDRAYGPGVADMKAGLVMNAFVLAAFRKFGAPVPVVGLITSDEEIGSPACRP
                     VIETAAREARAVLNSEPGRPSGNIVTSRKGGVFMRMTVEGRPAHSGGNFEKGISAIGE
                     LAHKIIAIHKLTDLAKGTTLNVGIMRGGEAVNMVAPSAEAEIDMRYVDPPERAKTMAA
                     IEEIVAASYVPGTRAALEIKGEFLPLNPTPSSEALFAHYQAAARDAGLKVEGEFAGGC
                     ADSGFTAAVGCPTICAVGPVGGAAHTPEEYLEIGSLVPRAQAMALAVMRMGAA"
     gene            2770..3780
                     /locus_tag="VF226_12400"
     CDS             2770..3780
                     /locus_tag="VF226_12400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074835.1"
                     /translation="MSKLDITVVGAGIFGLWQAFELTRRGHRVMLREASEESAVGAAS
                     RYAGAMLAPYCEAESAEPVIQDLGRTGVAMWREAYPGVVYRGTLVVAGSRDRSELNRF
                     ARLTQGHREISESELAELEPDLAGRFSRGLYYEEEGHLAPRAALPYLVSELRRMGATL
                     RFGDPVPEPIWMAASNGGVVIDCRGLAARGDLPDLRGVRGEMAVLHAPEVTLTRAVRL
                     LHPRQPLYVVPWGNHVYMVGATLLEREDGAPVTVRSALELLGLAYALHPGFAEATVEE
                     LGAGVRPSFRDNIPRAIVRGRRILVNGAYRHGYLFAPVMANAVANYLEHGTVNPAVMR
                     IE"
     gene            complement(3801..7220)
                     /locus_tag="VF226_12405"
     CDS             complement(3801..7220)
                     /locus_tag="VF226_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HEX7074836.1"
                     /translation="MFQQLMTSRRFAPLFWCQLFSALNDNFLKNALVILILYHTATGQ
                     SGALVTLAGGVLVAPFLLLSALGGELADKFDKAIVARKLKLAEVPIAGVAAAGFLLNS
                     IPLLFLALTLFGVMAALFGPIKYGILPDHLETRELPAGNALVEGATFVAILAGTLAGG
                     LAAAEQGNATLVAIIVLGMAVLALLFAQAIPSTGNSASGLRINPNPVSSTAALLRDLK
                     ADHRLWIGGLIVSWFWLVGVIALSLLPTLTKEQIGGTAQVVTLGLVVFTVGIAAGSVA
                     AAQASRLRPNLALVPVGALLMGLFSLDLAWSAAGITPGPTATTPGELLATGSGIRLTF
                     DLFALAAAGGLFIVPSFAAVQAWAPVDRRARVVAAVNVLNAAFMTSASLGVSALQYAG
                     LSAPQLLAGLGIANLLVVGLVLWAWGKQGVQDMASFVFRLCYRLEVKGLEIVPPAGER
                     VIITPNHVSLMDGPVMHAILPSHAAFAVDTAISQAWWVKPFLKLINAHTMDPTRPMAT
                     RTLVNLVKQGETLVIFPEGRVTVTGGLMKVYDGAAMIADKADAWVVPVRIDGLERSSP
                     WSYLRASQIRKSWFPKVRVSILPPRKLAVPPELKGKARRQAAAAALQDVMIDTAVATT
                     PIDKTLFEALADAKATRCTGKPIVADALGAKLSYAKLILAAQVLGRKLEAMAPQGAAV
                     GVMLPNSAGLGVTFFALQSIGRVPAMINQTTGLQNVLAACRAAQVEVVLTSRVFVEKA
                     RLQELVERLSLNVKVVFLEDVRARITIADKMVGLLLGSRPQVVRDCNDPAVILFTSGS
                     EGTPKGVVLSHRNILANVAQSLTRIVANGEDTVFNALPLFHSFGLTAGLVMPLMAGAP
                     VFLYPSPLHYRIVPELVYQTNATILFGTDTFLNGYARVAHPYDLHSVRLVLAGAEAVK
                     QRTRDLYMEKFGVRILEGYGLTETAPVLASNTPLTNKAGTVGRLSPLMEVRLDPVPGI
                     DDGGRLFVRGPNVMLGYYRAENPGVLEPPEDGWHDTGDIVSIDPQGYLTLKGRAKRFA
                     KVGGEMVSLAVVESFALELWPGAVSVAVAVPDLRKGERLVLLTTQADATRDAILRHAR
                     TKGASELMVPSAVLCVPKLPLLGSGKPDFTAAQKMAEELISAKSSSGVVAA"
     gene            7465..>8349
                     /locus_tag="VF226_12410"
     CDS             7465..>8349
                     /locus_tag="VF226_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7074837.1"
                     /translation="MQRLTASLVSMIAAATFVVLPGGAAAETTLRFAHTQPTADTHHA
                     AALHFKERVEALTNGEVKVSVHPAGELGNDPAILEGVRLGTIDIANTGNPFFTRFEPR
                     LNVLDLPYSFKSYEHAYRIIDGPIGQELIDGLQKHRMQPLAFWEIGFRNITNSKRAIR
                     TPQDLEGLKIRTTPNPAHVQAFKILGAIPTPMPFTEVYLALESGTVDGQENPLAIIHS
                     NRFHEVQKHLSMTRHAYTVSITVMNQQKFNSLSEEHRKALLQAAKEAQQLQRKLNREA
                     DKTALEEFRKAGVEVIEEI"
BASE COUNT         1586 a         2733 c         2682 g         1348 t
ORIGIN      
        1 aaggccctcg ccgctatgtg caggagtagg tgagccatcg ggctcgcggc atgctcagat
       61 cggcgagggt cttgtccatc gggcgggggc gacagtcgcc accccgcgga tggaacaaga
      121 agcgggcgtc cgtaaatggc caagcgcgac tactacgagg tcctcggcgt atccagaaac
      181 gcgagcgagc aggaactgaa gtccgccttc aggcggctgg ccaaggagtg ccatcctgat
      241 cacaactccg gcgacaaaag cgccgagcag cgtttcaagg aaattgccga agcctacgag
      301 actctgaagg atccccagaa gcgcgcagct tacgatcagt tcggccatgc cgcgttcgac
      361 ggcggtggcc gcggggcagg cggtttcggg ccggacttcg ccgcctccat gtccgacatc
      421 ttcgacgatc tgttcggaga gttcatgggc ggccgacggg gccgccaacg gtcagggcgc
      481 gagcgcggcg ccgacctcag gtacaatctc gagattaccc tctcggaggc ctacaccggc
      541 aagacggctc agatccgcgt gccgtccagt gtcgcctgcg agaaatgctc cggttcgggc
      601 gcaaaacctg gcaccaagcc atcgagctgc ccgacgtgtg ccggcttcgg caaggttcgt
      661 gcctcgcagg gcttcttcac gatcgagcgc acctgcccga cctgccaggg gcgcggcgag
      721 gtcatcgacg atccgtgccc ggcctgctcc ggctcgggcc gcgtcatgcg cgaacggacg
      781 ctgtccgtga atattcctgc gggtgtcgag gatggcacgc gcatccgcct tgccggtgag
      841 ggcgaagcgg gcctgcgcgg cggtccggcg ggcgacctct atattttcct ctcgatcaag
      901 cccgacgagt tcttccagcg cgatggtgcc gacatcttct gcatggtgcc gatctcgatg
      961 tgcaccgccg cgctcggcgg ccagatcgag gtgcctaccg tcgacggcag cgtcacgcgc
     1021 gtgaaaattc ctgagggcac ggagagcggc aagcagttcc gcctcaagaa caagggcatg
     1081 ccggtcctgc gctcgaaatc ctatggcgac atgtacatcc aggttcaggt ggagactcca
     1141 aagaacctga cccgtcgcca acgggagctc ctcgaggagt tcgagcgcga aagccacaag
     1201 gacaccagcc ccgagagcgc cggcttcttc tcccgcgtca aggagttctt cgagggccgc
     1261 ggcgtctgaa gtcactgccc ctctcagagt tttccaaccg ctaccctcat tccgtcatcc
     1321 cggcgcttgt cgccgggatc cacctaacca cggactccgc actcacggat agctggatcc
     1381 cgggcacaag gcccgggatg actgtcgtgt tttgtcctgg acgcaataaa cgggaccatc
     1441 gcccccagcg cggaatgctc tacaatggcc tcacatcatt ccatccagtg gggcacccaa
     1501 tgacactctc cgagcgcgaa gcgcagatta cggcatggct ggccgagcag aaggacgcca
     1561 tgatcgccct gctgcgcgaa gtggtgaata tcgactccgg cacctacgac aaggcaggtg
     1621 tcgacgccgt aggcgcacgc ttcgagcaat tctttgccga acatggcctg cccacccggc
     1681 gcgagcccaa cgagcgcttc ggagacgcca tccacattcg cctcgacgac cggctgacga
     1741 acgagaaacc ggttttgctg ttgggccatc gcgacacggt tttttccaag ggcgaagctg
     1801 agcggcgacc gttccgtatc gaaggcgacc gggcctacgg ccccggcgtt gccgacatga
     1861 aggccggact cgtcatgaac gccttcgtgc tggccgcttt ccgcaagttc ggcgcgcccg
     1921 tccccgtcgt cggcctcatc accagcgacg aagagatcgg ctcacccgcg tgtcggcccg
     1981 tcatcgagac cgcagcccgt gaggcccgcg cggtgctcaa ctccgagccg ggacggccca
     2041 gcggcaacat cgtgaccagc cgcaaaggcg gtgttttcat gcgcatgacc gtcgagggcc
     2101 ggccagcgca ctccggtggc aatttcgaga agggcatcag cgccatcggc gagctggcgc
     2161 acaagatcat cgccatccac aagctgaccg atctcgccaa gggcacgacg ctcaacgtcg
     2221 gcatcatgcg tggcggcgag gcggtgaaca tggtcgcccc atcggccgag gccgagatcg
     2281 atatgcgtta cgtcgatccg cccgagcggg cgaagacgat ggccgcaatc gaggagatcg
     2341 tggccgccag ctacgtgccg gggaccaggg cggcgttgga gatcaagggc gagttcctgc
     2401 ctctcaaccc gacgccctcc tccgaagccc tgttcgcgca ttatcaggcc gccgcccgcg
     2461 atgccggcct gaaggtggag ggagagtttg caggcggttg cgccgactcc ggattcacgg
     2521 ctgcggtcgg ctgcccgacg atctgcgccg tcggccccgt tggcggcgcg gcgcatacgc
     2581 cagaggaata tctggagatc ggctcgctcg tcccgcgtgc gcaggcgatg gcgttggccg
     2641 tgatgagaat gggggcggct tagccgggct cgctgcggcg tatggctctc ccggggaatg
     2701 gcccgatgtc atcgcactcc gggcggtgct aaacccattt cggcaaagcg atctcccaag
     2761 ggtggtgctg tgagcaagct cgacatcacg gtggtcgggg ccggcatctt cggcctctgg
     2821 caggccttcg agctgacccg ccgggggcat agggtcatgc tgcgggaagc ctcggaggaa
     2881 tctgccgtcg gcgcagcaag ccgctacgcg ggcgccatgc ttgccccata ttgcgaggca
     2941 gagtctgcgg aacccgtgat ccaggattta ggccgcaccg gtgtcgcaat gtggcgcgag
     3001 gcctaccctg gcgtcgtcta tcgcggcacc ctggtcgtcg ccggcagccg ggaccgcagc
     3061 gagctcaacc gcttcgcgcg tctcacccag ggccatcgtg aaatttccga gagtgagctg
     3121 gcggagctcg agccggatct cgccggacgg ttcagccgcg ggctctatta cgaggaggag
     3181 ggccacctcg ccccgcgggc ggccctgccc tacctggtct cggagctgcg ccgaatgggc
     3241 gccacgctgc gattcggaga tccggtgccg gagccgatct ggatggcggc atcgaacggc
     3301 ggcgtggtga tcgactgccg tggacttgcc gcgcgcggcg acctgccgga cctgcgcggc
     3361 gttcgtggcg agatggccgt tctgcacgca cccgaagtga cgctgacgag agccgtccgc
     3421 cttctccatc cgcgccagcc gctctatgtc gtgccatggg gaaaccatgt ctacatggtc
     3481 ggcgcgacct tgctcgagcg cgaggatggt gctccggtta cggtacgttc ggcgctcgag
     3541 ctgctcggcc tcgcctacgc tctgcaccca ggatttgcgg aggcgacggt ggaggagctg
     3601 ggcgccggtg tgcggccgtc gttccgggac aatattcccc gtgcgatcgt ccgaggccgg
     3661 cgcattctgg tcaacggcgc ctatcggcat ggctatctct tcgcgccggt catggccaac
     3721 gctgtcgcca actacctcga gcacggcacg gtcaacccgg ccgtcatgag gatcgaatag
     3781 cgcgggaccc tgctctgcag tcacgcggca acgacaccgc tcgacgactt tgcagagatc
     3841 agctcctccg ccatcttctg cgctgccgtg aaatccggct tgccggagcc gagcagcggc
     3901 aatttcggca cgcagagcac ggctgagggc accatcagct cggaggcgcc tttggtccgg
     3961 gcatggcgca gaatcgcatc gcgcgtggca tccgcctgcg tcgtcaacag aacgagccgt
     4021 tcgcctttgc gcagatcggg gacggcaacg gcgaccgaaa cggcacccgg ccatagctcg
     4081 agggcgaagc tctccacgac ggccagcgag accatctccc cgccgacctt ggcgaaccgc
     4141 ttggcccggc ctttgagcgt gagatagccc tgcggatcga tcgagacgat atcgcccgtg
     4201 tcgtgccagc catcctccgg cggctcgagc acacccgggt tctcggcacg atagtagccg
     4261 agcatcacgt tcggcccgcg cacgaacagg cgtccaccgt catcgattcc gggcacagga
     4321 tcgagcctga cctccatcaa aggcgacagc cggccgaccg tgcccgcttt gttggtgagc
     4381 ggcgtattgc tcgcgagcac cggcgcggtc tcggtgaggc catagccctc cagaatgcga
     4441 acgccgaact tctccatgta gagatcgcgg gtgcgctgtt tcacggcctc ggccccggca
     4501 aggacgaggc gcacggaatg caggtcgtaa gggtgcgcga cccgcgcgta gccgttgagg
     4561 aacgtgtccg tcccgaacag gatcgtggcg ttggtctggt agacgagctc cggaacgatc
     4621 cgatagtgca gcggggaggg gtagaggaac acgggcgccc cggccatcag cggcatgacg
     4681 agccccgccg tcaggccgaa cgaatgaaac agcggaagcg cgttgaagac cgtgtcctcc
     4741 ccgtttgcca cgatccgggt cagcgattgc gcgacattgg ccagtatatt ccggtgggaa
     4801 agcacgacac ctttgggcgt cccttcggat ccggacgtga acagaatgac ggccgggtcg
     4861 ttgcaatcac gaacgacttg cggccggctg ccgagcagca gccccaccat cttgtcggca
     4921 atggtgatcc tcgcccgcac atcctccagg aagacgacct tcacgttaag ggaaagccgc
     4981 tcgacaagct cctgcagacg cgccttctcg acgaaaaccc gcgaggtgag cacgacctcg
     5041 acctgcgcgg cccggcacgc tgccagcacg ttctggaggc cggtcgtctg attgatcatc
     5101 gccggcacac ggccgatgct ctgcagagca aagaacgtca cccccaaccc ggccgagttt
     5161 ggcagcatga cgccgacggc cgcaccctgc ggagccatcg cctcgagctt gcggccgagc
     5221 acctgggcag cgaggatgag cttggcgtag gagagcttcg cgcccagcgc gtcggcgaca
     5281 atgggcttgc cggtgcagcg ggtggccttc gcatcggcga gcgcctcgaa gagcgtcttg
     5341 tcgatcggag tggtcgccac ggcggtgtcg atcatcacgt cctgtagcgc agcggcggcg
     5401 gcctgacggc gagccttgcc ctttagctca ggcggcaccg cgagcttacg cggcggcagg
     5461 atcgacacgc gcaccttcgg aaaccaggat ttcctgatct gggaggcgcg caggtagctc
     5521 cagggtgaac tgcgctcgag cccatcgatg cgcacgggca cgacccaggc atcggccttg
     5581 tcggcaatca tggcggcgcc atcgtagact ttcatcagcc caccggtgac cgtcacgcgc
     5641 ccctctggga agatgaccag cgtctcgccc tgcttcacca gattgaccag cgtccgcgtc
     5701 gccatcggcc gcgtcggatc catggtgtgg gcgttgatga gcttcaggaa cggcttgacc
     5761 caccacgcct gcgaaatcgc cgtgtcgacc gcgaacgccg cgtggctcgg caggatcgcg
     5821 tgcatcaccg ggccgtccat caggctgacg tggttcggcg tgatgatcac ccgctctccc
     5881 gccggcggaa caatttccag ccccttcacc tcgagccggt agcagagccg aaaaacgaac
     5941 gacgccatat cctgcacgcc ctgcttgccc caggcccaca gaaccaagcc gaccaccagc
     6001 agattggcga tcccgagccc ggccagcagc tgcggcgccg atagtcctgc gtactggagc
     6061 gcggacacgc cgaggctggc agacgtcatg aaggcggcgt tgaggacgtt gacggcggca
     6121 acgacgcgcg cgcgtcggtc aactggcgcc caggcctgca cggcagcgaa cgacggcacg
     6181 atgaaaagtc cgccggcagc ggcgagcgcg aacaggtcga aagtcagccg tatccctgag
     6241 ccggttgcga ggagctcacc gggtgtcgtg gctgtcgggc caggcgtgat cccggcggcg
     6301 ctccaggcaa gatccagact gaacaggccc atcagcagcg cgccgacagg cacaagcgca
     6361 aggtttggcc gcaaacggct cgcctgcgcc gccgcgaccg atcccgcagc gatgccgacg
     6421 gtgaaaacga ccaggcccaa tgtgaccacc tgcgccgtac cgccgatctg ctccttggtc
     6481 agcgtcggaa gcagcgacag tgcgatcacg ccgaccagcc agaaccagga cacgatgaga
     6541 ccgcctatcc agagacgatg atccgctttc agatcgcgga gcagcgcagc agtcgagctc
     6601 accggattcg ggttgatgcg caggccggag gccgagttgc cggtcgacgg gatggcttgc
     6661 gcgaagagga gcgcgagcac cgccatcccg agcacgatga tggcgacgag cgttgcgttg
     6721 ccctgttccg ccgccgctag cccgcccgcc agggtcccgg cgaggatcgc gacgaacgtt
     6781 gccccctcga ccagcgcatt gccagccggc agctcccggg tttccagatg atccggaagg
     6841 atgccgtact tgatcggacc gaacagcgcc gccatcacgc cgaacagcgt cagggccagg
     6901 aacaggagag gaatggaatt cagcaggaag cccgcggccg cgacacctgc gatcgggacc
     6961 tctgccaatt tcagcttccg cgcgacgatc gccttgtcga acttgtcggc aagctcaccg
     7021 ccgagcgccg acagaaggag gaacggcgcg acgagaacac cgccggctag cgtgacgagg
     7081 gcgccactct gcccggtcgc ggtgtggtag agaatgagaa tgaccagcgc gttcttcaga
     7141 aaattgtcgt tgagcgccga aaagagctga caccagaaga gcggcgcgaa ccgccgcgag
     7201 gtcatcaatt gctggaacat gtgagttctc gctgaaatgg accagcctga gcactatcgg
     7261 gaaaattctg gttaaagaac ggctctgacg tactgaccag agcagtctgt ttcgcacccg
     7321 gcgcactcga aagaggggcc ggcgcatcac tgccggccgg caaggaaaaa gagacttgcg
     7381 ccgcaatctt tttgactttt ctgcctcggg tgaggtatca gcgccacacg gtacccagca
     7441 caagcgaaac aaagggaaac agggatgcaa cgcctgactg cctcactcgt gtccatgatt
     7501 gctgcggcca cattcgtggt acttcccggt ggtgccgccg cggaaacaac tcttcgcttt
     7561 gcgcacaccc agccaactgc cgacacgcat cacgccgccg cgctgcattt caaggagcgg
     7621 gtcgaggccc tcacgaatgg cgaggtcaag gtttcggtgc acccggcggg cgagttgggc
     7681 aacgatccgg caatactcga aggcgttcgg ctcggaacca tcgatattgc gaacaccggc
     7741 aatccgttct tcacccgctt cgagccgcgg ctcaacgtgc tggatctacc ctattccttc
     7801 aagagctatg aacatgccta ccggatcatc gacggcccga tcggccaaga gctgatcgac
     7861 ggcctccaga agcaccggat gcagccgctg gccttctggg agatcggctt ccgcaacatc
     7921 acgaacagca agcgtgcgat ccgtacgccg caggatctcg aaggcctgaa gatccgcacg
     7981 acgccgaatc ccgcccacgt ccaggcgttc aagatcttgg gagcgatccc aacaccgatg
     8041 ccgttcactg aggtctacct cgctctggag tccggtacgg tcgatggaca ggaaaatcca
     8101 ctcgcgatca ttcactcgaa ccgtttccac gaggtgcaga agcacctgtc gatgacgcgg
     8161 catgcgtata ccgtgtccat cacggtcatg aaccagcaga aattcaatag cttgagcgag
     8221 gagcaccgga aggcgctgct tcaggccgcc aaggaagcgc agcaactgca gcggaagctc
     8281 aatcgcgagg ccgacaagac ggcgctcgag gagttccgta aggccggcgt cgaagtgatc
     8341 gaagagatc
//
LOCUS       DASVDU010000193         5626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_418204, whole genome shotgun sequence.
ACCESSION   DASVDU010000193 DASVDU010000000
VERSION     DASVDU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418204"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF226_12415"
     CDS             <1..181
                     /locus_tag="VF226_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074838.1"
                     /translation="GLLGAPARIAQAGAPLAFGVLIETYGSGALLWSSAICLSALLAF
                     CAVHEKPSPPDEALG"
     gene            244..1005
                     /gene="arsH"
                     /locus_tag="VF226_12420"
     CDS             244..1005
                     /gene="arsH"
                     /locus_tag="VF226_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HEX7074839.1"
                     /translation="MRVRELPDPDFLPALDRRYALPRPALGLPHGGMDHPPRILLLYG
                     SLRQRSYSRLVVEEAARLVRYYGGEPRIFDPSDLPLPDQVKSDDHPAVHELRQLAMWS
                     EGQIWCSPERHGQITSVMKAQIDHLPLNMGGLRPTQGRTLAVMQVCGGSQSFNAVNTL
                     RLLGRWMRMFTIPNQSSVPKAFQEFDAAGRMRPSSYYERIVDVVEELVRFTVLVRPHV
                     EVLTDRYSERKEAAGNPRDVASDLSAIAIAHQRRA"
     gene            1039..1210
                     /locus_tag="VF226_12425"
                     /pseudo
     CDS             1039..1210
                     /locus_tag="VF226_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845582.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1763..2050)
                     /locus_tag="VF226_12430"
     CDS             complement(1763..2050)
                     /locus_tag="VF226_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074840.1"
                     /translation="MTPFYFLVDAQPGDNPSRDAARLRTIARGVFVEKVLGNVLDPGV
                     WLDNFHDPARAHTANGAVGPIEDNVTTALLSPPATRRETPISTPYEVQKTI"
     gene            2318..3523
                     /locus_tag="VF226_12435"
     CDS             2318..3523
                     /locus_tag="VF226_12435"
                     /inference="COORDINATES: protein motif:HMM:NF017116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HEX7074841.1"
                     /translation="MELEAWLADRQWFVKRNRLSYELEIHGPNGIIPITDERLAEIRF
                     TVAYASNNKEPAKDKIADAVSLIGERRAYHPVLDYLAGLRWDGVHRLDHWLVDYFGAE
                     DTPLNRAFGRKILCAAVRRVMHPGCKFDAMLVLEGAQDLGKSSGIRALCPDQAWFTDQ
                     LEIGADPKITIERTAGAWLVEMPELDGLGRRDTNRVKSFISTTHDRARLAYGRFAVTR
                     PRQFVLFGTTNESRYLSDLTGNRRFWIVRVIKADPTEIAAIRDQLWAEAVQAERAENL
                     WLDDPELKAAAAEVVREASDFGPWLELLESRIPEGALKIPAVELWKLVGIDGAESINK
                     LTKAHHAHMRAAMAGLGFERRDAGLRDEAGRKVKAYLRGDPHRARWWRPGDPIHEGPP
                     DEPPSLYDW"
     gene            4166..5500
                     /locus_tag="VF226_12440"
     CDS             4166..5500
                     /locus_tag="VF226_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEX7074842.1"
                     /translation="MHATPFLPGLSPVGSKPLTATFDAGRLSSDGGLIVLREIATRLR
                     LAETITNPLRDARDPTRVRHSYAEMVAARMLMIAAGYEDCDDVDTLRTDPALKIAVGR
                     APETGAELMSQPTLSRLENLADWRALARIGLGQIDLYCRSFARPPARIVLDIDDTDDP
                     VHGQQELALFNAHYDCTCFQPILIFDGLSGKPVLSLLRPGKRPSGEEVAKVLRHVIRR
                     IRRRWPGVAILVRGDSHYCSEPALALLEAMHCDYIIGFAINSKLLEIAAPWRAKCDMR
                     RSRMKPIVRRFHQLPYRAREWSRSRKLVARVEATSLGTDVRFIATSLEGRAKTLYEKV
                     FCARGKAENLIKDMKRFTRSDKTACSRWQANQFRLFLHVGAYWLLHQLRGAAPKRSRW
                     RGATFETIRRTFVKIAVRVEELKGKIKLAFPASYPQASMLVAMTGAITTRGP"
BASE COUNT         1063 a         1745 c         1725 g         1093 t
ORIGIN      
        1 cgggctgctc ggcgcaccgg cccgcattgc gcaagccggc gcccctttgg cattcggcgt
       61 cctgatagag acctacgggt ccggcgcatt gctctggtca agcgctattt gcctttcggc
      121 gttactggcc ttttgcgcag ttcacgagaa gccaagtccc cccgatgaag cgctgggctg
      181 aatgtttccg cgacgccaat gtcgcccgct gccccctcac caacaaccca atggtgaaag
      241 atcatgagag ttcgtgagct tcctgaccct gatttcctgc ccgcgctcga caggcgctat
      301 gctcttcctc gcccggcgct cggattgcct cacggcggta tggaccaccc accccgtatt
      361 ctgttgctct atggctcgct ccgacagcga tcatactctc gtctggtcgt agaagaagcg
      421 gcgcgcctcg tacgctacta tgggggcgag ccgcgcatct tcgatccttc cgatctaccc
      481 ctcccggacc aggtaaagtc ggatgaccac cctgccgtgc atgaattgcg ccagcttgca
      541 atgtggtcgg aaggacagat ctggtgcagc ccggagcgac atggccagat cacgagtgtc
      601 atgaaggcgc agattgacca cctgccgctc aacatgggcg gccttcggcc cacgcagggg
      661 cggacactgg ccgtcatgca ggtttgcggc ggctcgcaat cattcaatgc ggtgaacacg
      721 ctcaggctcc tcgggcgctg gatgcggatg ttcaccattc cgaaccagtc gagcgttccc
      781 aaggcattcc aggagttcga tgccgccggc cgaatgcggc cctcaagtta ttacgagcgg
      841 attgtcgatg tagtcgagga actggtgcgc ttcacggttc tggtacggcc acatgttgaa
      901 gttctgaccg accgctactc cgagcgaaag gaagcggctg gtaacccccg cgacgtcgcg
      961 agcgacctgt ccgcgatcgc cattgctcat cagcgaaggg cataaagcgg cgcaccagtt
     1021 cgtctgcctc aaactgcccg cgcggcgatc gcggtgctac tgccgctagt gacagaggtc
     1081 tggcacgtct acgttctgat ttttcgtgct cgcctatgac ctggaaagcg tcgtcagtgc
     1141 aatgttggcg gcggcactgc ttaccgtcgt caacttccat ggcttgttgg tggggacggt
     1201 gattggcttc ccatcgcagg atcatacctg ttcgccgtgc taaccattcc cggtttatgg
     1261 acatgcccgg taattgactg gagggcatat ccgtgactga caccttgaaa gcagagatcg
     1321 acacatcaag gctcccacca cgcatcaagc gcgccgaagc gtctgcatac cttaagcagc
     1381 gccacggtct cgaacgcacg cccggtactc ttgccaaact tgcgtgcctt ggtggcgggc
     1441 ctgcctttcg taaagtaggc gcgcggtgtg ttctctacga catcgaggaa ctcgatcgat
     1501 gggctacaga aataattggg acgccccgcc gttcgaccag tgaagccact gcgcagacct
     1561 agaggtcggg gcaactccaa aatggaacgc ccccggctcc aattcttgaa accgggggtg
     1621 gttgaggggt gaggtgtagt tcgcagcgtg gcgctgcaat aattacagaa ttcctcgccc
     1681 gtcgcaaact cacatatcta agcgaaaggc gaaaaaaatg aatatccact tcagtagggg
     1741 tgaattcctc tccgacgcca actcagatgg ttttttgcac ctcatacggc gtacttattg
     1801 gtgtttcgcg ccgcgtggct ggtggactga ggagagcggt agtcacgttg tcttcgatcg
     1861 ggcctaccgc cccatttgcc gtgtgcgcgc gggccgggtc gtggaaattg tcgagccaga
     1921 cacctggatc gagcacattg ccgagcacct tctccacaaa gacaccccgc gctattgtgc
     1981 ggaggcgcgc cgcatcgcgg ctggggttat cgcccggctg ggcatccacg aggaaataaa
     2041 acggcgtcat aagattggtc gtctcccgtt tgccacgccg ccagtggggt ggcgtccgca
     2101 tcggaagagc cggtgacgac cgatggacgt tgatccactc gacataccgg tgcctggccg
     2161 tggcggggct gctggaggtg ctccttgtcc tgccagatct gccccagcgc caagctcgtc
     2221 gcggccggcc cctgaagcgc gctctgtgct cgctatacgg agcgcgcctg ggccattggg
     2281 ccgcgtaggc caaatcatgg gcgtggaccg gtttgacgtg gagcttgagg cttggttggc
     2341 cgatcggcag tggttcgtca agcgcaatcg gctgtcctac gagctggaaa ttcacggccc
     2401 caacggcatc atccccataa ccgatgaacg gctcgcggag atccgcttta cggtggccta
     2461 cgcctcgaac aacaaagagc cggcaaagga caaaattgcc gatgccgtgt cgctcattgg
     2521 tgagcggcga gcgtatcatc cggttttgga ttaccttgcc ggcctgcgct gggacggcgt
     2581 tcaccggctt gatcattggc tggttgacta cttcggggcg gaggacacgc ccctgaatcg
     2641 ggcgttcgga cggaaaatcc tgtgcgcagc tgtccgccgc gtgatgcatc ccggctgcaa
     2701 gttcgacgcg atgctagtgc tggagggcgc gcaggatctc ggaaaatcct ccggcatccg
     2761 cgccctgtgc cccgaccagg cctggttcac ggaccagctt gaaatcggtg ccgaccctaa
     2821 gatcacgatc gaaaggacgg ccggggcatg gctcgttgag atgcccgagc tcgatggcct
     2881 ggggcgacgc gacaccaatc gggtgaagtc cttcatctcg accacacatg acagggcgcg
     2941 gctcgcatat ggccgatttg cggtcacccg accgcggcaa ttcgtactct tcggaacgac
     3001 gaacgaaagc cgctacttga gcgatctcac gggcaatcgc cggttctgga tcgttcgggt
     3061 tatcaaggct gatcctaccg aaatcgcggc catccgcgac cagctgtggg ctgaagccgt
     3121 gcaggcggag cgtgctgaaa atctgtggct cgacgatccc gagctgaaag ctgctgcagc
     3181 agaggtggtg cgcgaagcgt cggacttcgg accatggctg gaactgctcg aaagccgcat
     3241 ccccgagggg gcccttaaga tcccagccgt tgagctttgg aagctggtcg ggatcgatgg
     3301 tgcagaaagc atcaacaaat tgacgaaggc acatcacgcc catatgcggg ccgcaatggc
     3361 gggacttggt ttcgaacgga gagacgcagg ccttcgagac gaagccggaa ggaaggtgaa
     3421 ggcgtacctt cgcggcgatc cgcatcgcgc tcgttggtgg agaccaggtg atcccataca
     3481 tgagggaccg cctgacgaac cgccatcgtt atacgactgg tgaagttcgc ttcatcgcga
     3541 aacccggcag gtgatccttg ccggcttttt tttcatgcga ccagttccgc gggaaccggt
     3601 ttgtcgtgct cgatggcggg actggaacag gttctaaagt ttctggcatc ggttcggcct
     3661 gagccggttc caggctaggt cattgattct aagtcattag aaccagtggg actggtggaa
     3721 ccggttattt ctctataagc gctggaaccg ccagtttgtt gcgaaactag caatgcatcc
     3781 ggaagctttt tgaaattgga tcggaaccgg tgccaacaag ttccaggtgg cagcagcacg
     3841 gaagatgagg tggaaagatc gcgctccaat ttgacgatag cgttgtgatt tgctcagcaa
     3901 ttccgccaat ccgcgatcgt ccctggagtc agggaaacgc cgcttgatcg catcctggcg
     3961 gcctactcga cctatctact ggggcccggg agaatgtgag caccacgcag ctgtcgcgcc
     4021 accttctcgc cgttgcacct agcccatctt attcacgatc cagtgcggga tgagatgatg
     4081 ggcggggacg aagccgttgg tttggcgtag cattctggtg tcgaagcaga gtgtgcgtcg
     4141 ccagagccca ccaccgccca tcgccatgca cgctacgccg ttcctgcccg gcctgtcacc
     4201 agtcggatca aagccgctca cagccacgtt cgatgctggc cgtctgtcct cggatggcgg
     4261 cctgatcgtg ttgcgcgaga tcgcgacccg cttgcggctt gccgagacga tcaccaaccc
     4321 gctccgcgac gcgcgcgatc cgacccgcgt ccgtcacagc tacgccgaga tggtcgcggc
     4381 gcggatgctg atgatcgcgg ccggctacga ggactgcgac gacgtcgata cgttgcgcac
     4441 cgatcccgca ttgaagatcg ccgtgggtcg ggcgccggag accggcgccg agctgatgag
     4501 ccagccgaca ttgtcgagac tggagaacct ggccgattgg cgcgcattgg ccaggatcgg
     4561 cctcggccag atcgatctct actgcagaag cttcgctcgc ccgccggcgc gcatcgtgct
     4621 cgatatcgac gacaccgatg acccggtgca cggccagcag gagctggcgc tgttcaacgc
     4681 gcactacgac tgcacctgct tccagccgat cctgattttc gacggcctct cgggcaagcc
     4741 cgtgctgtcg ctgctgcgcc ccggcaagcg gccctcgggc gaggaggtgg cgaaggttct
     4801 gcgtcacgtg atccggcgca tcagacggcg ctggcccggc gtcgcgatcc tggtgcgcgg
     4861 cgacagccat tattgcagcg agccggcatt ggcgctgctg gaggccatgc actgcgacta
     4921 catcatcggc ttcgccatca actccaagct gctggagatc gccgccccct ggcgcgcgaa
     4981 atgcgacatg cgccgctcga ggatgaagcc aatcgtacgc cgcttccatc agcttcccta
     5041 tcgggcccgc gagtggagcc ggtcgcgcaa gctcgtcgcc cgcgtcgaag cgacaagcct
     5101 tggcaccgac gtgcgcttta tcgccaccag cctcgaaggg cgcgcgaaga cgctctacga
     5161 gaaggtgttc tgcgcccgcg gcaaggccga gaacctgatc aaggacatga agcgcttcac
     5221 gcggtccgac aagacggcgt gctcgcgctg gcaggccaat cagttccgcc tgttcctgca
     5281 cgtcggcgcc tactggctgc tgcaccagct tcgtggagcg gcgccgaagc gctcgcgctg
     5341 gcgcggcgcc acgttcgaga cgatccggcg cacgttcgtg aagatcgccg tgcgcgtcga
     5401 ggagctgaag ggcaaaatca agctcgcctt tccggccagc tatccccagg cctccatgct
     5461 cgtcgccatg accggtgcca tcacgacgcg cggcccgtga ctgacgcggc gtgtgccgcg
     5521 cgagccccga acatcacccc caacgcgttc cgaacgttcc cgaaaaccgc cgccgtcaac
     5581 ccggcagacg acccgtgcgt ccaaccactc ggcccggtca ttacga
//
LOCUS       DASVDU010000194         7207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1173277, whole genome shotgun sequence.
ACCESSION   DASVDU010000194 DASVDU010000000
VERSION     DASVDU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7207
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173277"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF226_12445"
     CDS             <1..202
                     /locus_tag="VF226_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7074843.1"
                     /translation="PAQVVLRWHIQQPGVVAVPKSGNPKRIAENIDVFNFALDDADMQ
                     RLSALARPDGRVVNLEFAPEWD"
     gene            328..1551
                     /locus_tag="VF226_12450"
     CDS             328..1551
                     /locus_tag="VF226_12450"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074844.1"
                     /translation="MSRSSSNASALFAVPDYRRLWVIGAGLGVARWLEFLALGVFAYQ
                     VTGSPPLVALVAIVRMLPNVFLGLVVGALADHLDRRRMLATGFLLGAVAYGVMAGVAF
                     AGAAGYGTVILAATASGLMWTLDMPVRRRLLVDAAGIQRMTAALGFDNSTNYATRAIG
                     PIAGGAAYQAFGIEGIFALSAAIYSFCFALCTRLKTADPPEAHEKEEARISLPSLLVP
                     PRELLWSRRFQVVLGVTVVFNIWCFPIISMVPVLGEKEFALSPVLIGMLSASEGLGGT
                     VGAVMIGLMAGQRPPSFQLYYFGVFAFVVLLLVMSAWLTLGVAVICLLLIGAAAAAFS
                     ATQYALVYTMSPPKLRGRAAGFMSIVIGTSTIGFYNTGFLFSRFDSANALMLMGLQGL
                     VPIVILGVLWLRAKS"
     gene            complement(1514..2626)
                     /gene="pqqE"
                     /locus_tag="VF226_12455"
     CDS             complement(1514..2626)
                     /gene="pqqE"
                     /locus_tag="VF226_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX7074845.1"
                     /translation="MTHPLPLGLLAELTHRCPLACPYCSNPVELDRRSDELDTKTWIR
                     VFEEAAELGVLQLHLSGGEPTARADIVAMTRGASRAGLYTNLITSGINLTKARLAELA
                     EAGLDHVQLSWQDTDPARADEIAGLAGAHDWKQQTARAVNELGLPLTINVVVHRLNID
                     RVADMIVAAHRAGARRIEVAHTQYYGWALANRAALLPTPEQVEAAHAAVARIRQELEP
                     SILIDYVAPDHFSRWPKPCMNGWGRQSLNVTPRGKVLPCHAAESIRGLEFWNVQEHSL
                     REIWEDSPAFTAYRGTEWMKEPCRSCPRKEIDFGGCRCQAFAWTGDAANTDPACEYSA
                     HHAALRAAVVAPRTESLTYRRFSGLSSSPSAIARPE"
     gene            complement(2623..2898)
                     /gene="pqqD"
                     /locus_tag="VF226_12460"
     CDS             complement(2623..2898)
                     /gene="pqqD"
                     /locus_tag="VF226_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX7074846.1"
                     /translation="MKIAPETRPRLARGVRLHEDRVRRRTVLLAPERVVTPNRSAIEI
                     LRRCDGALTFNEIVADLARTFTAEPERIAADAEALLTDLANRRMVDL"
     gene            complement(3113..4360)
                     /locus_tag="VF226_12465"
     CDS             complement(3113..4360)
                     /locus_tag="VF226_12465"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7074847.1"
                     /translation="MPQRIVSRLPIVAALLTLSPAPAEAQDQQIVDTDLVRVRVETIA
                     TGLNHPWGIAMLPDGRYLVTERNSGDLRIGTRNGELSEPVEGVPDIFRYQGDTSSSQG
                     GLFHVALHPQFAENRLVYLSFSQPSSEGAGTAIARGRLEESGGSPALEDVEVIFTMNK
                     HDSGGLHFGGRFVFDPKDNTIYLSIGDRRNMSRAQDPLDHAGSIVRITDDGGTPSDNP
                     FVNDPDKDELIYSWGHRNIQGLAVDPETGELWANEHGPLGGDEINLIKAGRNYGWPMQ
                     TGGVDYSGAPIGRGAIVEGYEPPVHIWERTVAPSGLAVYAGSLFPQWRGDLLHGGLVA
                     EGLIRTRIVNGKVEQNELMLADLGRRIRDVEVDGEGAIWLVTDEEDGEVLRLVPEEPV
                     ATGTIPGQKPPAATGSIPRRQKE"
     gene            complement(4494..6152)
                     /locus_tag="VF226_12470"
     CDS             complement(4494..6152)
                     /locus_tag="VF226_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX7074848.1"
                     /translation="MNDQPFQTFDPLPDAAAPAASVGTPSRTSTLEREIARRRTFAII
                     SHPDAGKTTLTEKLLLFGGAIQLAGQVKAKRDRRNTRSDWMAIERARGISVVTSVMTF
                     EYRDKVFNLLDTPGHEDFSDHTYRTLTAVDAAVMVIDAAKGIEARTLKLFEICRLRDI
                     PILTFINKLDRETREPLELLDEIERVLALDTAPMDWPIGRGREFRGIYDLNHSRVRRI
                     DEDPDGFAVTGPDDPAITELMEPDQFARWREEVGLVIDACGRFDLEAFRNGTLTPVFF
                     GSALRNFGVRDLLDALVEYAPPPASLKADKRIVEATEPKMTGVVFKIQANMDPNHRDR
                     IAFMRVCSGRLTRGMRVKLTRTGKTMALQAPQFFFAQNRSIADEAYAGDVVGIPNHGT
                     LRIGDTLTEGEDLTFLGIPNFAPEILRRVRLDDAIRAKKLRTALQEMAEEGVVQLFNP
                     LDGSPPLIGVIGALQLDVLADRLGTEYNLPVAFETSPFELVRWITSEDKAALQSFIGK
                     NRSQIATDLDGSEVFLAPSQFMLQWTAERAPEIKFTDIKIMIGG"
     gene            6300..6650
                     /locus_tag="VF226_12475"
     CDS             6300..6650
                     /locus_tag="VF226_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEX7074849.1"
                     /translation="MTRKLDLDKYAERLRARRAELLKAQEETEADRGTVELDQTTVGR
                     LSRMHALQEQATALATARKREEELARIDAALKRIEEGDYGYCLVCGEEIDERRLDLDP
                     SLPTCIDCARSGHR"
     gene            6803..>7207
                     /locus_tag="VF226_12480"
     CDS             6803..>7207
                     /locus_tag="VF226_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX7074850.1"
                     /translation="MSSTGLEVFDKTLQTTNLWLNEIGQDIGPDKQRCYHALRAVLFA
                     LRDRLTVDEAADLSAQLPMLIRGIFYEGYRPSTMPQRIRTRDEFLAKINEHLQNIRPL
                     GADEAARAVFRVLDKNIPAGEMNEVKQMLPPEI"
BASE COUNT         1175 a         2270 c         2436 g         1326 t
ORIGIN      
        1 gccggcacag gtcgtcctgc gctggcacat ccagcagccg ggcgtggtcg ccgtgccgaa
       61 gtcgggcaat cccaagcgca ttgccgagaa catcgacgtg ttcaatttcg cgctcgacga
      121 cgctgacatg caacgtctgt cagcattggc gcgcccagat ggccgggtcg taaacctcga
      181 gtttgcgcct gagtgggact agagaggcaa ccccccgcca tgcatggttg ctccgacctg
      241 cccgccgggc ggggcaggtg acatgccctg aaatccgggc gatggtgggc cgctcgatac
      301 tctcctttgg gctgcaactc atctctcgtg tccaggtctt catccaacgc cagtgcgctg
      361 ttcgccgtgc ccgattaccg gcgcctgtgg gtcatcggcg ccggcctggg cgtggcgcgc
      421 tggcttgaat tcctggcgct cggggttttt gcctaccagg tcaccggctc gccacccctc
      481 gtggcgctcg tcgcgatcgt ccggatgctg cccaacgtct tccttggcct ggtcgtcggg
      541 gcgctcgccg atcacctcga caggaggcgt atgctggcga ctggtttcct gctgggggcc
      601 gtggcgtacg gcgtgatggc cggagtggca ttcgcgggcg ccgcgggcta cgggacggtc
      661 atcctggccg caacagcgtc gggcctgatg tggaccctgg atatgccggt ccggcggcgg
      721 cttctggtcg atgcggccgg tatccagagg atgacggcgg cgctggggtt cgacaactcg
      781 acgaattacg ccacccgtgc cattggcccg atcgccggcg gtgctgccta tcaggcgttc
      841 ggaatagagg gcatcttcgc gctgagcgca gccatctatt cgttctgctt cgctctttgc
      901 acgcggctga agacggccga tccgccggaa gcgcatgaga aagaagaggc ccggatttcg
      961 ctgccgtctc ttcttgtgcc gccgcgagag ttgctgtgga gccgccgctt tcaggtcgtc
     1021 ctcggcgtca ccgtggtctt caatatctgg tgctttccca tcatctcgat ggtgccggtg
     1081 ttgggcgaga aggagttcgc tctgtcaccg gtcctcattg ggatgctatc cgcgtcggag
     1141 gggctcggcg gaacggtcgg cgcggtgatg atcggcttga tggcgggcca acgacctcct
     1201 tcgttccagc tctattattt cggtgtcttc gcttttgtgg tgctgctcct cgtgatgtcg
     1261 gcctggctga cgctgggcgt agcggtgatt tgcctgctct tgatcggggc cgctgcggcg
     1321 gcgttctcgg ccactcaata tgcgctcgtc tacaccatgt cgccgcccaa gctgcggggc
     1381 cgggcggcgg gcttcatgtc gatcgtcatc ggcacgtcga ccatcggctt ctacaacacc
     1441 ggcttcctgt tctcgcgctt cgactccgcc aatgcactga tgctcatggg actccagggg
     1501 ctcgtcccga tcgtcattct gggcgtgcta tggctgaggg cgaagagctg aggccggaaa
     1561 accggcgata ggtcagcgac tcggtgcgcg gtgcaacaac agcggctcgg agtgccgcgt
     1621 gatgcgcgga gtactcgcac gccggatccg tgttggcggc atcgcccgtc catgcgaacg
     1681 cctggcagcg gcagccgccg aaatcgatct ccttccgggg acaggagcgg cacggctcct
     1741 tcatccattc ggtaccgcgg taggccgtga aagccggcga gtcctcccag atctcacgca
     1801 gcgaatgctc ctggacgttc cagaactcga gcccgcggat gctctccgcc gcatggcagg
     1861 gaagcacctt gccccgcggc gtcacgttga gcgattgccg gccccagccg ttcatgcacg
     1921 gcttcggcca acggctgaaa tggtctgggg cgacatagtc gatcagaatg ctgggctcga
     1981 gctcctgccg aatgcgggcg accgcagcat gggcagcctc gacctgctcc ggtgtcggca
     2041 ggagggcggc gcgattggcc agggcccagc cgtagtactg cgtgtgggcc acctcgatgc
     2101 gccgcgcgcc ggcccgatgg gccgcgacga tcatgtcggc gacacggtcg atattgagac
     2161 ggtgcaccac cacgttgatg gtcagcggaa gccccagttc attgacggcg cgcgccgttt
     2221 gttgcttcca atcgtgagca cccgcgagac cggcgatctc gtcagcgcgg gcggggtcgg
     2281 tgtcctgcca ggagagctgg acgtggtcga ggccggcttc cgccaactcc gcgagccggg
     2341 ccttggtcag attgatgccg gacgtgatga ggttcgtata gagccctgcc cgcgatgccc
     2401 cgcgggtcat cgccacgatg tcggccctcg cagtcggctc gccgcccgac agatggagct
     2461 ggagcacccc cagctcggca gcctcctcga acacccggat ccatgtcttg gtgtcgagct
     2521 cgtcggaccg gcggtcgagc tcgacgggat tagagcagta cgggcaagcc agcggacagc
     2581 gatgggtcag ctcagccagc aatcccaggg ggagcgggtg cgtcacaggt ccaccatgcg
     2641 tcggttggcg aggtcggtga gcaaggcttc ggcatcggcc gctatgcgct ctggctccgc
     2701 ggtgaacgtg cgggcgaggt cggctacgat ttcgttgaat gtcagcgcgc cgtcgcagcg
     2761 gcgcagaatt tcgatggctg accggttcgg cgtcaccacc cgctccggtg cgagcaacac
     2821 cgtgcggcgc cggacccggt cctcatgcaa gcgaacgccc cgcgcgagcc gcggccgggt
     2881 ctcgggcgcg atcttcatgc tggcctttta cacaagaggc ttcgctcgag agaagcgtta
     2941 ttggcgaagg ccgaagtcag cctccgctcg tttgcgtgat gtgggcgcgc gacagtgttt
     3001 cgtcggatgc gccgcaggct tatttgccgg cagtctcgtc cggcgagtag tagcccgtgg
     3061 cttcgaggcc aacggagcaa tcgtgcacag tcggtgcagt ccagttcatg acctactcct
     3121 tctgcctgcg agggatggac cccgtcgccg caggcggctt ctgcccaggt attgttccgg
     3181 tcgcaaccgg ctcctccggc acgaggcgga gtacttcgcc gtcctcctca tcagtcacga
     3241 gccagatcgc gccctccccg tccacctcca catcccggat gcggcggccg aggtcggcca
     3301 gcatcagctc gttctgttcc accttgccgt tgacgatgcg cgtgcgaatg aggccttcgg
     3361 caaccaatcc gccgtgcagc agatcgccac gccattgcgg aaacaggctt ccagcgtata
     3421 cggcgagtcc tgacggtgca accgttctct cccagatgtg aacgggtggc tcgtagccct
     3481 cgacgatcgc gccacgtccg atgggagcgc cggaatagtc gacgccgccc gtctgcatgg
     3541 gccagccata gttgcgcccg gccttgatca ggttgatctc gtcgccaccc agggggccgt
     3601 gctcgttcgc ccacagctcc ccggtttcgg gatcgacggc caggccctga atgttcctgt
     3661 gaccccagga atagatcagc tcgtccttgt ctgggtcgtt cacgaacggg ttgtcggacg
     3721 gggtcccgcc atcatcggtg atgcgaacga ttgagccggc gtggtccagt ggatcctggg
     3781 cgcgggacat gttccgccgg tctccgatgg agagatagat cgtgttgtcc ttcgggtcga
     3841 acacgaaccg gccgccgaag tgcaggccgc ccgagtcgtg cttgttcatg gtgaagatga
     3901 cttccacgtc ctcgagggcc ggcgagcctc cgctctcctc gagccgcccg cgagcgatgg
     3961 ccgtgccggc gccctcggag gagggctggg agaagctcaa gtaaacgagc cgattttcag
     4021 cgaactgcgg gtgcagagcg acatggaaca gaccgccctg gctggagctc gtgtcgcctt
     4081 gatagcggaa gatgtccggg acgccctcga ccggctcgga aagctcgccg ttgcgcgtgc
     4141 cgatacgcaa atcgcccgag ttccgctcgg taacgaggta tctcccgtcc ggcagcatgg
     4201 cgatgcccca ggggtggttc aggccggtcg cgatcgtctc gactcgaacc cggaccagat
     4261 cggtatcgac aatctgctga tcctgggcct cggccggggc ggggctgagt gtcagaaggg
     4321 cagcaactat gggaagcctg gaaacgatgc gttgcggcat aagtcgctcg gggcgcgcgc
     4381 aaaaagggac aagtccggac cggaaaaacg ctctgccgag caatcagttc cagtactgac
     4441 tttccacccc cggtggagct gccgctgccg tgcttgcgtc tccttggctt ggctcaaccg
     4501 ccgatcatga tcttgatgtc ggtgaacttg atctccggag cgcgttccgc cgtccactgc
     4561 agcatgaact gcgaaggggc gagaaagacc tcagagccgt cgaggtccgt cgcgatctga
     4621 gacctgtttt tgccgatgaa gctctggaga gcggccttgt cctccgaggt gatccagcgc
     4681 accagctcga atgggctcgt ctcgaatgcg accggcaggt tgtattcggt gccgagccgg
     4741 tcggcgagca cgtcgagctg cagcgcgccg atgacgccga tgagcggcgg cgagccgtcg
     4801 agcgggttga agagctgcac gacgccttcc tcggccatct cctgtagcgc tgtcctcagc
     4861 ttcttggcgc ggatggcatc gtcgaggcgg acccggcgca ggatctccgg ggcgaagttt
     4921 gggatgccga ggaaagtgag gtcctcgccc tcggtcaggg tgtcgccgat gcggagcgtg
     4981 ccgtggttgg ggatgcccac cacgtccccc gcataggcct cgtcggcgat agagcggttc
     5041 tgggcgaaga agaattgcgg cgcctggagc gccatggtct tgccggtgcg cgtgagcttg
     5101 acccgcatac cgcgcgtgag ccggccggag cacacgcgca tgaaggcgat gcggtcgcgg
     5161 tggttgggat ccatgttcgc ctggatcttg aacacgaccc cggtcatctt cggctcggtc
     5221 gcctccacga tccgcttgtc ggccttgagg ctcgcgggcg gcggtgcgta ctcgaccagt
     5281 gcatcgagca ggtcgcgaac accgaagttc ctgagcgcgc tgccgaagaa caccggggtc
     5341 agcgtgccat tgcggaaggc ctcgaggtcg aagcgcccgc aggcgtcgat cacgagcccg
     5401 acctcctcgc gccagcgggc gaactggtcc ggctccatga gctccgtaat ggccggatca
     5461 tccgggccgg tcaccgcaaa gccgtcgggg tcctcgtcga tgcggcgcac gcgggaatgg
     5521 ttgagatcat agatgccgcg gaactcgcgt ccccgcccga tcggccagtc catgggtgcc
     5581 gtgtcgagcg ccagcacgcg ctcgatttca tcgagcagct cgagcggctc gcgcgtctcc
     5641 cggtccagct tgttgatgaa ggtgaggatc gggatgtcgc gcagccggca gatctcgaat
     5701 agcttcagcg tccgcgcctc gatgcccttt gccgcgtcga tgaccatgac cgcggcatcg
     5761 acggccgtca gcgtccgata ggtatggtcc gagaagtcct cgtgccccgg ggtgtcgagc
     5821 aggttgaaga ccttgtcgcg gtattcgaag gtcatcacag acgtgacgac agaaatgccg
     5881 cgcgcccgct cgatcgccat ccagtccgag cgcgtgttgc gccggtcccg tttggccttg
     5941 acctggccgg caagctgaat ggcgccgccg aacagcagca gcttctcggt caacgtggtc
     6001 ttgccagcgt cggggtgcga gatgatggca aacgtgcgcc ggcgggcaat ctcgcgctcg
     6061 agcgtcgagg tgcgactggg cgtgccgacg gaagcggccg gcgcggccgc atccggcagg
     6121 ggatcgaagg tctggaacgg ctgatcgttc aagcggctta cccttgtatt ggggaaattg
     6181 gcggatgtca tgtaccatca ccggcgctgc agggcagccg tcggctgggc ggcgtggcgc
     6241 cacccccatg gctcgggatg ggcgccagtc catgagccct tcatatggag cagtcgtcga
     6301 tgacgcgcaa attggatctg gacaagtacg ccgagagact gagagcacgg cgcgccgagc
     6361 tgctgaaggc ccaggaggag acggaagccg atcgtggcac ggtggagctg gaccagacga
     6421 cggtcgggcg gctttcgcga atgcatgccc tccaggagca ggcgactgcg cttgcgaccg
     6481 cacgcaagcg ggaggaagag ctggcgcgga tcgacgcagc gctgaagcgg atcgaggaag
     6541 gcgattacgg gtactgcctt gtctgcgggg aggagataga cgagcgccgg ctcgacctcg
     6601 atccgtctct gccgacctgt atcgattgcg ctcgtagcgg ccatcgttga ggctcggcac
     6661 ccatctccaa gcggcgctcg cctgccggcc gccggttccg gaacctgcgt agtccccgaa
     6721 gcgttcgaga tggagacgac agctcggggg agacgggccg agaagacggc tcgtggagcc
     6781 cggcatggaa cggagccgag acatgagctc aaccggcctc gaagtattcg acaagacgct
     6841 gcagaccacc aatctctggc tgaacgagat cggccaggac ataggcccgg acaaacagcg
     6901 ctgctaccac gccctgaggg cggtcctgtt tgccctgcgc gaccggctca ccgtggatga
     6961 ggcggctgat ctttcagccc agcttccgat gctgatccgc ggcatcttct acgaaggcta
     7021 ccgcccctcg acgatgccgc agaggattcg cacgcgggac gagttcctgg cgaagatcaa
     7081 cgagcatctg caaaacatcc ggccgctcgg agccgacgag gcggcgcgcg ccgtcttccg
     7141 tgtgctcgac aagaatatcc cggccgggga gatgaacgag gtcaagcaga tgctgccgcc
     7201 ggagatc
//
LOCUS       DASVDU010000195        10472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1125049, whole genome shotgun sequence.
ACCESSION   DASVDU010000195 DASVDU010000000
VERSION     DASVDU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125049"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF226_12485"
     CDS             <1..288
                     /locus_tag="VF226_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX7074851.1"
                     /translation="MISDGAPVDDSTLSVNSGSYLEQHLRQVIEEIENRSPVELIAIG
                     IGHDVTRYYRRAVTITDPSELAGAMTDKLVELFEEKAATAPARPMRRRVLH"
     gene            358..1431
                     /locus_tag="VF226_12490"
     CDS             358..1431
                     /locus_tag="VF226_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX7074852.1"
                     /translation="MRRKAEARRFGPAVAALMLPLAVSLFVLATAAVAQKPLVTETKP
                     VAIAARPLAGFDREDLDRQDFGRLRWRGGLVLNSSAEEFGGWSGLVLDDQGRRLLAVS
                     DTGAWLTAELTYDGRRPAGLRRSWIGPLQALSGKPLTRTRDRDAESVALAEGTLKDGL
                     VLIGFEGNDRIGRFRMTEAGPSAPLHYVDLPPEARRMGSNGGFEAISVLKSGRLEGSM
                     IAFAERLLDEHGNHTGWVWVDGKPQVLHLVDIGGFDITDAAALDDGGLLILERRFRWT
                     EGVKMRLRLIPADEIAPGALMRGEVLFEGDMAYDIDNMEGVAVHRDPHGETVVTLLSD
                     DNFNPILQRTLLLQFTLKDKPED"
     gene            complement(1469..1780)
                     /gene="rpmB"
                     /locus_tag="VF226_12495"
     CDS             complement(1469..1780)
                     /gene="rpmB"
                     /locus_tag="VF226_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7074853.1"
                     /translation="MTRRCELSGKLPMSGQLRSHAENKSKRTFRPNLCTVSLISDTLK
                     RTIRLRVSAHALKSVEHRGGLDAYLLKAKDEELSQRCRRLKRDIQKAKASAGSEATAA
                     A"
     gene            2025..2882
                     /locus_tag="VF226_12500"
     CDS             2025..2882
                     /locus_tag="VF226_12500"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7074854.1"
                     /translation="MSTVPARLFSWMNSSSCLAGLALLGTFLPFAAAASGITPSKVEA
                     SYSIEFAGIEIGKFRFQSSVDASTYTLSGSAKFSALLGAWKWSGRTSSTGTLGQGEPQ
                     PARYVFKYKSNSKSGSVDMSFSKGAVADRTLEPPSKPSKKTVPLRDEHLRNVLDPMSA
                     VMAMTTGQIDKPCDRKIAIFDGKQRFSLELSFSRKERIKEARPSGEPEVAIVCGVRYI
                     PIAGYKDSKVIRYMAEAKGIEVALRPIPSANILVPYEIRIPTFAGSAILKSQRVEITT
                     GVKQIALVH"
     gene            3086..6370
                     /locus_tag="VF226_12505"
     CDS             3086..6370
                     /locus_tag="VF226_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7074855.1"
                     /translation="MGIDYESRIRNVTAVLGPTNTGKTHLAIERMLGHETGMIGLPLR
                     LLAREVYDKIAARVGADRVALITGEEKIKPEHPRYWVSTVEAMPREIDVDFLAIDEIQ
                     LAADPERGHVFTDRLLHARGKLETLLLGAGTMREAISDLIPGANFISRPRLSKLTYSG
                     QKKITRLPARTAVVAFSANEVYAIAELIRRQRGGAAVVLGALSPRTRNAQVAMYQSGD
                     VDFLVATDAIGMGLNLTVDHVAFASIRKFDGQSHRTLTPAELAQIAGRAGRHMNDGTF
                     GVTGDVEPFENDLVERLETHTFDTVKVLQWRNRQLDFSSLDALRDSLRAFPSEARLVR
                     ARMADDVIALENVSLDRELVDMVRGPAATAILWDVCQVPDYRKISSQNHAELVATLYK
                     FLVTGEERIPEDWFARQVALADRTDGDIDTLANRIAHIRTWTFVSNRSSWLRDPEHWQ
                     GRTREIEDSLSDALHEQLTQRFVDRRTSALMRGMRDKEALNADIGDDGAIHVENHYVG
                     RLKGFVFSPDAQAEGIHGKAARSAAAQVLAKELSMRTRRVASAKNDAFKLSRQGRILW
                     RGEEVARLEPGEHPLKPQLVLLADEHLQAPDKEKVQDRLTTWLNDTIRERLKPLVELS
                     EAQDLSGLARGIAFRLTENFGVLKRETVADEMKQLDQPGRAQLRRFGVRFGAFNIYFP
                     LLLKPAPAELTLTLWALKHAGEHGLSLDTLPEPPRAGLTSAPADPNVPEVFYRACGYH
                     VCGPRAVRIDILERLADLIRPLLAWRPNPENPSATPPKGSTGDGGFVVIPEMMSILGC
                     SPDELSAVFTSLGFRLDRRPVKPAPAPELPAGLQQPGASGEAQSEGAVAAEVSGSSTD
                     EPGVPTEPSDAAVEGHSAESVAAEPEPTPVETPEVALEDPAEPVDAVPASGVSAADGQ
                     PVGAPEPQGAAVILSAASEQPAGEAKPAEAEEIKYEDVWRPRRRHNGARPDRRHHRGR
                     HDGGQREERHGQGHQQARPQRQNQRRRDRQPAAAASAAQGGAARHSDAALGGGHKHRD
                     RKDARRDGRRGDDRRRNNERHGASVQIRSAAPPKRGEVDPDSPFAALSALKKALEKQA
                     QEPSSS"
     gene            6367..6792
                     /locus_tag="VF226_12510"
     CDS             6367..6792
                     /locus_tag="VF226_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7074856.1"
                     /translation="MSRSPAGAPEVGGGGQRLDKWLWFARVVRTRSLATALVLGGKVR
                     VNRVRVDKPSQTVRPGDVVTVTVHSRVRVLKITAAGERRGSATIAQMLYEDLTPPALG
                     SDRGQGGNHEPGPPQRDPGSGRPTKRERRAIERLTKKDG"
     gene            6904..7242
                     /gene="fdxA"
                     /locus_tag="VF226_12515"
     CDS             6904..7242
                     /gene="fdxA"
                     /locus_tag="VF226_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEX7074857.1"
                     /translation="MTFVVNDQCIKCKFTDCVEVCPVDCFYEGENMLVIHPDECIDCG
                     VCEPECPVEAIKPDTEPGLEKWLELNREYADKWPNITSKKPPPPDAEEYRDMEGKFER
                     FFSPKPGTGD"
     gene            complement(7275..8435)
                     /locus_tag="VF226_12520"
     CDS             complement(7275..8435)
                     /locus_tag="VF226_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7074858.1"
                     /translation="MDFELTAEQRALQETARSFAREQMAQVARDIERTNAPLSKEWKR
                     RYAEMGFLGINIAPEYGGLGLGNLEAILVLEEFAKVHPAVAFPIFESAVGPIKAVERF
                     GSETLKKWLLPKVTSGEAVIAVAMSEPNAGTALTDLSTSAELRDGRIVLNGTKRWCTG
                     GGSADGYVVYCRLSEAPGAKGIGAVFVPNGVKGLTFGAREELMGFRGTTTADIFFDNV
                     EVPEDHLIVPAGGFPKLMEAFDLERCGNATMCLGIAAGALEDALAYVQERRQFGKPIV
                     EFQAVQLKLAEMAMKVDAARLLIYRAVTRASAGFPSIADSSIAKCFANEMVREVAGAA
                     VQLMGGYGYSKQFAAEQRLRDAWGWGIAGGTIDVQKINIASALVGRRFDQRK"
     gene            complement(8525..9808)
                     /locus_tag="VF226_12525"
     CDS             complement(8525..9808)
                     /locus_tag="VF226_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7074859.1"
                     /translation="MDALVLLGTFGLLIAIGVPVAFSLGLAAIAGAIWYDIPLQAVML
                     KISDGVDEFALLAIPFFVLAGAIMAEGGMALRLVNLAKVFVGFIRGGLALVNVLASTF
                     FGCISGSSVADTASVGSVMIPQMEKNGYPRVFATTVTVCGSVQAILIPPSHNAVIYSL
                     AAGGTISIAHLFLAGVLPGLLLGLTLMTMILVMAHVRQYPKGEVVSLRQAGKICVDAF
                     WGLLTMFIILGGILSGVFTPTESAAVACVYAFIVTMFVYRDYKWRELPLLVARVVRTV
                     GMVMMLIGFAAAFGYMMALMRLPAQVTQFFLSVSDNKYVILLLINAMLLLLGCLMDMA
                     PLIVICTPILLPVAVNIGIDPVHFGMILLVNLGMGLITPPVGATLFVGCAVGRVTMGE
                     MVRTIWPFYLAMLVVLALVTYVPAISMWLPSLMKL"
     gene            complement(9837..10364)
                     /locus_tag="VF226_12530"
     CDS             complement(9837..10364)
                     /locus_tag="VF226_12530"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7074860.1"
                     /translation="MFAYYVRAMDVLHRICMVTAGLCLLIITIIIPWGVYTRYVLGYG
                     SQWPEPIAIILMIVFTFFAGAVCYRENLHIAVMALPSVTSGIARTILGWIIELCMLGT
                     SLFLVIFGWELVGTTWNQVLAQMPEIPVGLTYAPIPVGGAIISLFVIERLWSGRFFRP
                     AEAETPFESASADMN"
BASE COUNT         2098 a         3256 c         3334 g         1784 t
ORIGIN      
        1 atgatctcgg acggcgctcc ggtcgacgac tcgacgctgt cggtcaattc gggctcctat
       61 ctggagcagc acctcaggca agtgattgag gaaatcgaga accgctcgcc agtggagctg
      121 atcgccatcg gcatcggaca cgacgtgacg cgatattacc gccgcgccgt gaccattacg
      181 gacccgtccg agctggccgg cgcgatgacg gacaagctcg tcgaactctt cgaggagaaa
      241 gccgcaacgg caccagcgcg cccaatgcgt cgacgcgttt tgcactaggc tgacgcaccg
      301 gaaattttaa ctccggacct gcacaatccg agtgctattg gacaggaggg agcggcattg
      361 cgtcggaagg cggaagcgcg ccggtttggc cctgccgttg cggcgctgat gctgccgctc
      421 gccgtgtccc tgttcgttct cgccacggcg gcggtcgccc agaagccact tgtcacagag
      481 accaagccag ttgcgatcgc ggcccggccg ctggcagggt tcgacagaga agacctcgat
      541 cgccaggact ttggccgatt gcgatggcgc gggggtctcg tcctcaactc atctgcggag
      601 gaattcggcg gctggtcggg actggtgctc gatgaccagg ggcgccgtct tctggccgtg
      661 tccgataccg gtgcgtggct cacggcggag ctgacttatg acggcaggcg gccggcaggg
      721 ctgcggcgat cctggatcgg gccattgcag gcgctgagtg gaaaaccgct gacacggacc
      781 agggatcggg atgccgaatc ggtcgcgctt gcagagggaa ctctcaagga cgggctcgtg
      841 ctgattggat tcgagggcaa cgaccgcatc ggccggttcc gcatgaccga ggccggacca
      901 tcggctccgc tccactacgt cgatcttcca ccagaggccc gccgcatggg ctccaatggg
      961 ggattcgagg cgatcagcgt tctcaaatcg gggcgcctgg agggttcgat gatcgccttc
     1021 gcggagcggc tcctggacga gcacggcaac cacaccggct gggtctgggt cgacggtaag
     1081 ccgcaggtgt tgcacctcgt cgatatcggc gggttcgaca tcaccgacgc cgcagcgctc
     1141 gacgacggtg gtctcttgat cctcgaacgc cggttccgct ggaccgaagg tgtcaagatg
     1201 cggttgcggc tgatccctgc ggacgagatt gcacccggcg cgctgatgcg cggcgaggtt
     1261 ctgttcgaag gcgatatggc ctacgacatc gacaacatgg agggtgtcgc cgtgcaccgg
     1321 gacccgcacg gcgagacggt cgtcacgctg ctttcggacg acaacttcaa tccgatactg
     1381 cagcgcacgc tactgctgca gttcacgttg aaggacaagc cggaagacta aaggccgcta
     1441 agccagatgg taggctcggg cgtcgggatc acgcggccgc tgtcgcttcc gagcccgcgc
     1501 tggcctttgc cttctgaatg tcgcgcttca ggcgccggca acgctgggag agctcctcgt
     1561 ccttggcctt cagcaggtag gcatcgagac cgccgcggtg ctcgaccgac ttcagcgcat
     1621 gcgcgctcac cctcagccgg atcgtccgct tcagcgtgtc gctgatcagc gaaaccgtgc
     1681 acaggttcgg ccggaacgta cgcttgctct tgttctcagc gtggctccgg agctgtccgg
     1741 acatcggcag cttgccgctc agctcacagc gtctggtcat gacacgactt cctttcgtgg
     1801 cgcaccttgg cagggacgca tgaattctgc tcgtttcgga atcgtaaacg tcccgtcagg
     1861 atcgcggccg ggagttcgcg gccttctata cttaggccgc aagggaacag tcaagcgcgt
     1921 ggatcctaat gatctttccc atggggctga ggccagcgac aaacatttga ccaaaaagaa
     1981 acgtacggcg atgatctgag atgtcgcttc ccggaggcgt caccatgagc accgtacctg
     2041 cccggctctt cagctggatg aattcgtcca gctgtctcgc ggggctggcg ctgctcggca
     2101 ccttcctgcc ttttgcggct gctgcgagcg ggataacgcc cagcaaggtc gaggccagct
     2161 acagcatcga gtttgccggc atcgagatcg gtaagttccg ctttcagtcc agcgtcgacg
     2221 cgtcgaccta tacgttatcc gggagcgcga aattctcggc gctgctcggc gcctggaagt
     2281 ggtcgggaag gacgagcagc accggtacct tgggccaggg agagccgcag ccggcgcgtt
     2341 acgttttcaa gtacaagagc aattcgaagt ccgggtccgt cgacatgagc ttcagcaagg
     2401 gagcggtggc cgatcgaact ctggagccgc cgagcaagcc ctccaagaag acagtgccgc
     2461 tgcgcgatga gcatttgcga aacgtgctcg acccgatgag tgcggtcatg gccatgacga
     2521 ctggccagat cgacaaacct tgcgaccgaa agatcgccat cttcgacggc aagcaacgct
     2581 tcagcctgga gctgtcattc agccgcaagg agcgcatcaa agaggcgcgg ccgagcggcg
     2641 agccggaagt cgcaatcgtc tgcggcgtgc gctatattcc catcgcaggg tataaggaca
     2701 gcaaggtgat tcggtacatg gcggaagcca agggaatcga agttgccttg cgtccaattc
     2761 cgagcgccaa tattcttgtt ccttatgaga tcaggattcc aacctttgct ggatcggcca
     2821 tcctcaaatc acagcgcgtc gagattacga ctggggtgaa gcagatcgca ctggtccatt
     2881 gaccggaccg gcgactggta aactgaaatc gcgccttctc tgggaaggaa tgggcacacg
     2941 caggtctagc cgtttatttc gtcgggtcat ctcggcaagg acatcagatc tgcactgagc
     3001 ggtgacggga ctttgcgcga cattttagct tgcgtccggg gcgcctgcgt ctctcacgag
     3061 accaaggctg gaggctgagt tcatcatggg catcgattac gaatcgcgga tccggaacgt
     3121 caccgccgtt ctgggtccga cgaacaccgg caagacgcac ctcgccatcg agcgtatgct
     3181 cggtcacgag acggggatga tcgggctccc gctgcggctc ctggcgcgcg aggtctacga
     3241 caagatcgcc gcccgggtcg gtgcggaccg cgtcgccctg atcaccggcg aggaaaagat
     3301 caagccggag catccgcgct actgggtctc gaccgttgag gccatgccgc gggagatcga
     3361 cgtcgacttc ctggccatcg acgagatcca gctcgccgcc gatccggagc gcgggcacgt
     3421 cttcaccgac cgcctgctgc acgcccgcgg caagctggag acgctgctgc tcggcgccgg
     3481 cacgatgcgc gaggccatca gcgatctcat tccgggggcg aatttcatct ctcgcccgcg
     3541 gctttcgaag ctgacctact cgggccagaa aaagattaca cgcctgcccg cgcgcacggc
     3601 cgtggtcgct ttttccgcca acgaggtcta tgccatcgcg gagctgatcc gccggcaacg
     3661 gggtggcgct gcggtggtgc tgggagcgct gtcgccgcgg acacgcaatg cccaggtagc
     3721 catgtaccag tccggcgacg tcgacttcct ggtcgccacc gatgccatcg ggatggggct
     3781 caacctcacc gtcgaccacg tggctttcgc ctctatccgc aaattcgatg gacagagcca
     3841 ccgcacgttg acgccggccg agctggcgca aatcgcaggc cgtgccgggc gccatatgaa
     3901 cgacggcacg ttcggcgtga ccggcgacgt cgagccgttc gaaaacgatc tggtggagcg
     3961 gctggagacg cacacgttcg acaccgtgaa ggtgctgcag tggcgcaacc gccaactcga
     4021 cttctcgtcg ctggatgccc tgcgcgacag cctgcgcgcc ttcccatccg aagcgcgcct
     4081 tgtccgcgcg cgcatggccg acgacgtgat cgcgctcgag aacgtcagtt tggaccgcga
     4141 gctggtcgat atggtccgcg gccccgcggc gaccgccatc ctctgggacg tgtgccaggt
     4201 cccggactat cggaagatct cctcccagaa ccacgcggag ctggtggcga ccctctacaa
     4261 gttcctcgtc acgggcgagg agcgcattcc ggaagactgg tttgcgcgac aggtggcgct
     4321 ggctgaccgc accgatggcg acatcgacac gctcgcaaat cggatcgcgc acatccgcac
     4381 ctggacgttt gtttcgaacc gttcgagctg gctgcgtgat cccgagcact ggcagggccg
     4441 tacccgcgag atcgaggaca gcctgtccga cgcgctgcac gagcaactca cccagcgctt
     4501 cgtcgatcgc cggacgagtg cgctgatgcg ggggatgcgc gacaaggaag ctctgaatgc
     4561 cgacatcggc gacgacggcg cgatccacgt cgagaaccac tacgtcggcc ggctgaaggg
     4621 tttcgtgttt tcgcccgacg cccaggccga aggcattcat ggcaaggcgg cgcggagtgc
     4681 ggcagcgcaa gtgctggcga aagagctgtc gatgcggacg cgccgcgtcg catcggccaa
     4741 gaacgatgcc ttcaagctct cacgccaggg gcgcatcctc tggcggggcg aggaggtggc
     4801 gcggctcgag ccaggcgagc atccgctgaa accacaactc gtgctgctgg ccgacgaaca
     4861 tctacaggcg ccagataagg aaaaggttca ggatcggctg acgacctggc tcaacgacac
     4921 gattcgcgag cggctgaagc ctctggtcga gctctccgaa gcgcaggatc tttccggctt
     4981 ggcccgcgga atcgcgttcc gtctcaccga gaacttcggt gtgctgaagc gggagaccgt
     5041 cgccgacgag atgaagcagc ttgaccagcc cgggcgcgcc cagctgcggc gcttcggggt
     5101 gcggtttggc gccttcaaca tctatttccc tctgcttctc aaacccgcgc cggcggagct
     5161 caccctgaca ctctgggcct tgaagcacgc cggcgagcat gggctctcat tggatacctt
     5221 gccggagccg ccgcgcgctg gcctcacgtc agcgccggca gatccgaacg tgcccgaggt
     5281 cttctaccgg gcgtgcggct atcacgtgtg cggaccgcgg gcggtgcgca tcgacatcct
     5341 ggagcggctt gccgatttga tccggccgct tctggcctgg cggccgaacc cggagaaccc
     5401 ctccgccaca ccgccgaaag gttcgacggg cgacggcggc ttcgtcgtga tcccagagat
     5461 gatgtcgatc ctggggtgct ctcctgacga gctcagcgcc gtattcacga gccttggatt
     5521 ccgcctcgac cggcggccgg tcaaaccggc acctgctccg gaactgcccg ccgggctgca
     5581 gcagcccggt gcgtcaggtg aggcgcagtc ggagggggcg gtcgcggcgg aggtctccgg
     5641 gtcttcgacg gatgagcctg gtgtcccaac tgagccgtcg gatgcagcgg tagaagggca
     5701 ttcagcagag tccgtcgcgg cggaacctga accaacgccg gtcgaaacgc ctgaggtggc
     5761 gctcgaagat cccgctgagc cggtcgatgc ggtcccggca tcgggagtca gtgcggccga
     5821 tggccagccg gtgggtgcgc ctgagcctca gggggccgca gtcatactat cagctgcttc
     5881 ggagcagccg gccggagagg ccaagccggc ggaagccgag gagatcaagt acgaggacgt
     5941 ctggcggccc aggcgtcgcc acaacggagc gcggccggac cgccgtcatc accgcggacg
     6001 tcacgatggt ggccagcgcg aggaacggca cggtcaggga caccaacaag ctcggccgca
     6061 acgccagaac cagcgtcgcc gtgatcggca accggctgcc gcggcctccg cggcgcaagg
     6121 tggggcggca cggcattccg atgctgcact tggcggaggt cacaagcatc gggaccgtaa
     6181 ggacgctcgc cgcgacggcc gcagaggcga cgatcgccgc cgcaataacg agcggcacgg
     6241 cgcttcggtg cagattcgct cagctgctcc accgaaacga ggcgaggtgg atcccgattc
     6301 acccttcgcg gcattgagcg cgctcaagaa agcgctcgag aagcaggcgc aggagcccag
     6361 ctcatcatga gccgcagccc tgctggggcg ccggaagttg gtggtggcgg acagcggctc
     6421 gacaaatggc tttggttcgc gcgagtcgtg cggacccgga gtctcgcgac agcgttggtg
     6481 ctcggtggga aagtgcgcgt caatcgcgtg cgcgtcgaca agccgagcca gacggttcgc
     6541 ccaggcgacg tcgtcacggt aaccgtgcat tcccgcgttc gtgtgttgaa gatcacggca
     6601 gccggtgagc ggcgcggctc cgcaaccatc gctcaaatgc tctacgagga cctgacgcct
     6661 ccggctcttg gttcggatcg gggccaaggc ggaaaccacg agcctggacc gcctcagcgc
     6721 gatccgggct ccggtcgccc gacgaaacgg gagcgccgtg ccattgagcg attaacaaag
     6781 aaggatgggt gatctgttag tcctttgctc tggaacaatg aaaggcttgc gacaacctgc
     6841 caaagtgcgg agtgttgcag ccgagtcgta agacaagccc gaacaaacgg ggtgccgagc
     6901 aaaatgacct tcgtagtcaa cgatcagtgc atcaagtgca agttcaccga ctgcgtggaa
     6961 gtctgtcctg tcgactgctt ctacgaaggg gagaacatgc tcgtgattca ccccgacgaa
     7021 tgcatcgact gcggggtctg tgaaccggaa tgcccagtcg aggcgatcaa gccagacact
     7081 gagccgggcc tcgagaagtg gctcgagctc aatcgcgagt atgcggacaa gtggccgaac
     7141 atcacgtcga agaagcctcc ccctccggat gcggaggaat accgcgacat ggaaggcaag
     7201 ttcgagcgct tcttctcgcc gaagccgggt accggtgact aagcatcagt gagccgacgg
     7261 cgcgcgtggc acggtcattt ccgctgatcg aagcgcctgc ccaccagcgc tgaggcgata
     7321 ttgatcttct gcacgtcgat cgtcccgccg gcgatgcccc agccccaggc gtcgcgtaac
     7381 cgctgctcgg ctgcgaactg cttggagtag ccgtagccgc ccatgagttg cacagcggcg
     7441 ccagcgacct cgcgcaccat ctcgttggcg aagcacttgg cgatggatga atcggcaatc
     7501 gaggggaaac cggccgaggc gcgcgtgaca gcgcgataga tcagcaggcg cgccgcatcc
     7561 actttcatcg ccatttcggc gagcttcagc tgcacggcct ggaattcgac gatgggcttg
     7621 ccgaactggc gccgctcctg gacgtaggcg agcgcatcct ccaacgcgcc ggctgcgatg
     7681 ccgaggcaca tggtcgcgtt gccgcagcgc tccaggtcga aagcctccat cagctttgga
     7741 aagccccccg ccggcacgat caggtgatcc tccggcacct cgacattgtc gaagaagatg
     7801 tcggcggtcg tcgtgccgcg gaagcccatc agctcctctc gtgcaccaaa ggtcaggccc
     7861 ttcacgccat tgggtacgaa tacggcgccg atccctttgg cgccgggagc ctcggacaac
     7921 cggcaataga cgacgtagcc gtcagccgag ccaccgcccg tacaccagcg cttggtgccg
     7981 ttcaggacga tacggccgtc gcgcagttcc gccgatgtgg atagatccgt cagcgccgtc
     8041 ccagcgttcg gctccgacat ggcgaccgcg ataacggctt caccgctcgt cactttgggc
     8101 agaagccact tcttcagggt ctctgagccg aaccgctcca ccgctttgat cggaccgacg
     8161 gcgctctcga aaatcggaaa ggcgaccgcc gggtgcactt tggcgaattc ctcgaggaca
     8221 agaatcgcct cgagattccc gagacccaat cccccatatt cgggcgcgat gtttatgccc
     8281 agaaaaccca tttcggcata gcgtcgcttc cattctttcg agagcggtgc attcgtcctc
     8341 tcgatgtcgc gcgcaacctg ggccatctgc tcgcgcgcga aggagcgtgc ggtttcctgc
     8401 aatgcgcgct gctcagccgt cagctcgaaa tccaatgcgt cctcgccttc caatcaaatt
     8461 gaattgccga gcttcgtagc ctgttccctg ccattccaca atggattggt ccactgaggc
     8521 ggtttcaaag cttcatcagc gagggcaacc acatcgagat cgccgggaca taggtgacga
     8581 gcgccagcac gaccagcatg gccagataaa acggccagat cgtccgcacc atttccccca
     8641 tggtgaccct gccgacggcg catccgacga acagcgttgc gccgacgggg ggtgtaatca
     8701 accccatccc gagattgacg agcaggatca tgccgaagtg cacgggatcg atgccgatgt
     8761 tgacggcgac cgggagcaga atgggggtgc agatcacgat cagaggggcc atgtccatca
     8821 ggcagccgag gagcaggagc atggcattga tcagcagcaa aatgacgtac ttgttgtccg
     8881 aaacggacaa gaagaattgc gtcacctgcg caggcagccg catcagcgcc atcatatacc
     8941 cgaaggcggc cgcaaacccg atcagcatca tcaccatgcc gaccgtgcga acgacgcgcg
     9001 ccaccaacag cggcagctcg cgccatttgt agtcgcgata gacgaacatc gttacgatga
     9061 atgcataaac gcacgcgacc gcggcagatt cggtgggcgt gaaaacaccg ctgaggattc
     9121 cgccgagaat gatgaacatc gtcagcagac cccagaatgc gtccacgcag attttccccg
     9181 cctgccttag tgaaacgacc tctcctttcg gatactgacg gacgtgggcc atgacgagga
     9241 tcatcgtcat cagagtgagg ccaaggagca agccgggcag cacaccggcc aggaaaagat
     9301 gcgcaatcga aatggtgcca ccggccgcca gtgaataaat gacggcatta tgagagggcg
     9361 ggatcagaat ggcctgcacc gagccgcaga cggtgacggt ggtcgcaaag acacgcggat
     9421 agccgttttt ctccatctgc gggatcatga ccgagccgac agatgcagtg tccgcaactg
     9481 aggatccgga gatgcagccg aaaaaagtag aggcgaggac atttacgagc gccagaccgc
     9541 cgcgaatgaa cccgacgaaa accttcgcca agttgacgag ccgcaacgcc atgccgcctt
     9601 cggccatgat ggccccggcg agaacgaaaa acggaatggc gagcagggca aactcgtcaa
     9661 cgccgtccga gatcttcagc atcactgctt gcagcgggat atcataccag attgcgccag
     9721 caatagcggc cagccccagc gagaatgcca ccggaacgcc gatggcgatg agaaggccga
     9781 atgttccgag gaggaccagt gcatccataa cgagctattc cgggaagcgg cgcggatcag
     9841 ttcatatccg ctgaagcgga ctcgaacggc gtttcagctt cggcaggtct gaagaagcgc
     9901 cctgaccaga gccgctcgat gacgaacaat gagatgatgg cgccgccaac cgggatgggg
     9961 gcatacgtca aaccgacggg tatttccggc atttgtgcca gcacctgatt ccaggtcgtc
    10021 cccaccaatt cccagccaaa aatgaccaga aacagcgacg tgccgagcat gcacagctcg
    10081 atgatccagc cgagaatcgt tcgagcgatg cccgacgtca cgctgggaag cgccataacc
    10141 gcgatgtgca ggttctctcg gtagcagacc gccccggcga agaacgtaaa cacgatcatg
    10201 agtatgatcg caatcggctc aggccactga gagccatagc cgagaacgta acgcgtatag
    10261 acaccccagg ggatgatgat ggtgatgatg agcaggcaca gccctgccgt caccatgcag
    10321 atgcggtgca ggacgtccat cgccctcacg tagtaagcga acatgcaatc cgattccgat
    10381 gaaggagagg ccgagatgac gagagccctg ggactctctt cgctgcagct gtggcgagcg
    10441 cgcctccgcc ggctcaaatt gggcggaagg cg
//
LOCUS       DASVDU010000196        29080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1589135, whole genome shotgun sequence.
ACCESSION   DASVDU010000196 DASVDU010000000
VERSION     DASVDU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 29080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29080
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1589135"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(900..1247)
                     /locus_tag="VF226_12535"
     CDS             complement(900..1247)
                     /locus_tag="VF226_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074861.1"
                     /translation="MSNITDAVIALLEHRNEAGSQLEEMAAALIDNYLTESNENSVFW
                     EPKFRDPHGFNFDLNGFLKWEQLGRSLDQTPCLQAQLHFQKQFMGMSLGGARAARKMI
                     ADHLGSHAAGILD"
     gene            complement(1283..2827)
                     /locus_tag="VF226_12540"
     CDS             complement(1283..2827)
                     /locus_tag="VF226_12540"
                     /inference="COORDINATES: protein motif:HMM:NF022062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HEX7074862.1"
                     /translation="MGSTVSFDDFKTSWLETITAGAPSSVEVGRRFAHKIVSQWLDID
                     DDTLDVIYCDGSGDGGIDVAVLDRGGPPGPDDVPEGDTWYLVQSKYGSAFAGEGTLLV
                     EGRKVIETLDGSRTKLSCLASGLREKLTNFRDSAGEHDRICLVYATINPLTPDEQRTL
                     EDVRAMGRHRLGALFDVAAISIRTIYDAQAEESELANRRHLTFELHGHLTQAGTDLLV
                     GSVSLIDLYAFLKSYQGRTGAIDQLYEKNVRRFLGGRVKVNKGIYATLRESPEKFGLF
                     NNGITITVSDFKILGAGKYELIEPYVVNGCQTTRSIHEVLSSRLDAGGSGKNEKLESW
                     RARVEKGCVVAKIAKVGSEGDDLLQQITQFTNSQNAVRERDFVAINQGFKAWHLELAD
                     RYGLYLEIQRGGWDSQRAFQRQNPTTRQFTHFASALNLTKVYGAGWLAEPGLAFGKNP
                     PFLPNGSVFTRMTSERPGERPFGARDLYAAFCCGNLENRKALGAAVIRRDDKPSFSII
                     SCSLIC"
     gene            complement(3332..4846)
                     /locus_tag="VF226_12545"
     CDS             complement(3332..4846)
                     /locus_tag="VF226_12545"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX7074863.1"
                     /translation="MFGWRRKSDGFEWHEHVRTTIRIRREDRRRRIDEARALAAEGLS
                     SAGRAGMAAGTFGLNFLGQCARRLGEAAREGVALVISLLPIIGEAIRTGLTRALTAAA
                     EGSAKALLVLLDFSRRALILAFEALMDFGRRLNKFAYAAARILGIGVDAIGQTSFRLL
                     VIGCKSLRHSTALPLMVLVGVVASVAAIIRLATSGTSADAVFALVIGGACLIGALLVR
                     MLGDSTVSLSTSGYDLALLPQEQRKPYRFRVPLPAQTGRAAGLAAAVLVACSALIGGG
                     WLVWTGASHVVSAASSTGLFAEKIEGRAEAVAGDVLRIGGREIKLAGIEAPEPAQRCA
                     IPGNRSWRCGAAARIALSRMIRGKSVSCSVSGTDEKGRSLGTCVAGGDDIASMLVAGG
                     HVFAETGLFAKYDAEEEKARAEKAGIWRGNPERPAEYRARVEKELARAWEAAKRRAPR
                     GCPIKGNIIRGRKYYVLPGTPEYRRVRIETRRGERWFCSEEEAIAAGWKRSPRG"
     gene            complement(4994..7294)
                     /locus_tag="VF226_12550"
     CDS             complement(4994..7294)
                     /locus_tag="VF226_12550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7074864.1"
                     /translation="MTHLLALPAGTELVGEYRIDRVLGAGGFGITYLAEELALGREVT
                     IKEYFPGDFAARNGSNEAVPRSRESEGDYQWGLDRFIAEAQTLAMFDHRNIVRVYRYF
                     RANNTGYMVLHFEEGQSLKGWLQSLGRAPRQFELDQIVAPLLDALEVIHNANFLHRDI
                     APDNIIIRRDGAPVLIDFGSARGDIARHSRTVSALVKPGYSPYEQYAETGSQQGPWTD
                     IYALAATLYHAITGKRPPDAPSRIVKDELVPAREAALSSYRPGFLRAIDQALSLDIKK
                     RPQSIPEWRGPLLAPEPRKAGWVQRTIGKVAAEEDPPAKTLKLDTAAPPPPDAPGAKG
                     GLIEYIERLRRQSSPAATASAPTAAVEQPKSAEQPNKPAPKVDLPSPAEKPRARNRPA
                     KPARARQPWKWPQLPRLPRFSLPRLPRIRPFGGWRWRPLILKLMIGLGVAAFAVGLQD
                     RLPGFFMSADATRTGTASDIDQPRQIAGHSGGTSAVAFTDDGRRIVTAGEDRTLKIWD
                     VATGSNERTIGLGESRVTALAVRGSRAVTGREDGTLQLWDLAHGERLAQFQRNSASIW
                     SVTFMGDAEHFAASGHDWTVAVWDARFQAPAVTIEAHKSAVQDVAFSPNGGLLASGGA
                     DRLVKLWDASSGELIRTFRGHRDFVSAIAFSPDGKLLASAGHDGQIRLSRTSSGRLYR
                     VLSRRAEVVHGLAFSPAGSLLAAASADGRVRIWSIRRGRLLRSLGDGSAAYRSVAFSP
                     DGRWLAAGSDSGSVTLWNVSRLRDRR"
     gene            7612..8595
                     /locus_tag="VF226_12555"
     CDS             7612..8595
                     /locus_tag="VF226_12555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7074865.1"
                     /translation="MRSTAAIGLAALLFCFWTHSVAQAQPAQCKAGPAAIGVSRTVEI
                     DASTGPRFGRSQYRDFDFLQDGEVVLTFDDGPSRRHTPAILDALDAHCTKATFFSVGR
                     MAVADPEMLQETAMRGHTIGTHTWSHKNLRSLSLAQARAEVELGLSAVQAALGGPVAP
                     FFRFPYLSDPQPVQAMLQQRGIATFSIDADSYDFRTSSPRMVQENILNQLAQRKKGIL
                     LFHDIQPSTARALKGLLDELKARGYRVVHIVPKAPATTLADFDEMAEQVLRKKQVAAS
                     EHPLARHPASAPLAHSSGASRPRPSHSAPRQNHTRQRYRDDRSWIERVFQN"
     gene            complement(8602..10209)
                     /locus_tag="VF226_12560"
     CDS             complement(8602..10209)
                     /locus_tag="VF226_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX7074866.1"
                     /translation="MNDKTRLLRLGSASFDDAFQSALRDDGLEVRFTRSESRLLAHMV
                     RNAGRVLSRNQLLDAISEPGSDKSDRNIDFTINRLRRKLNDDPKNPRFIATRYGEGYI
                     WVAKALSGRPLASGAYVVVGPLRGLSHIGAFKSFAIGFARTFELRLGRHFSPDRKVVL
                     DPDCPPRSSFGDDCPEIAVDLTFLCDGVGLDCVFRARAFRTGRLFCVERHRIATAEDQ
                     VAGQLATADALADRIASSIWRNLAAHVEHHEPLPVQLHRASATLVGGHISWQESERRL
                     RQVLAEDPNDHIAKLMLATSIHTKYIENGIDILMAGLDCRSADEDEIEALVTASLPFI
                     QAEPYYAIMAAKLLHFIDRGYRRMAVELAENAHRSSTSPAASLAIIGQMRSFEGQIDQ
                     GLSSLEQALELSEPNSQFWLYLLVMKAQSQLAAGDRAGLDATLAQLYQGRPELKSFMD
                     IMFARHDEPSREALAVVQSASKARARATLLFVYYINARLFRYEDHRENVLRTPVLLFA
                     GRFGSSIVPVEVAACAPGVLPSGGRAG"
     gene            complement(10587..11798)
                     /locus_tag="VF226_12565"
     CDS             complement(10587..11798)
                     /locus_tag="VF226_12565"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEX7074867.1"
                     /translation="MTRFQGVPLWAPRALSLISLALSPQVAHADSNFVPTTIREAIGV
                     ERGSTALIGPVEKNTYFITFDTFGSEDIGPSILSTAAALPDLFVQGDQFDAVAVLGGP
                     FETGRQELLAGGLGYRIKLPNSGPTLFVNGDYADIRLGAPANRAIDATGQNWTITVGA
                     RQDWVVSQGSKLTGELSFTARQWKGQAFDRPVLDEDLRVLSARFSYAGRTPLGIRQRF
                     GVILHKGRTGLGSSDTHNPMASLPGASSDFLTVSFSADLAVPLTEQVVATIGAIGQYS
                     DAPLPFSQRCGYGTNEFSRAFDRAFVNGDECLGGRGEMAYNLPAADLSGGLLKFLQLF
                     AAADAGWLWNIRSDVAPASQDTWASAYLGVRAATSNFIAEASLSRTVDEPDGIVEQKG
                     TRLWVRSAMRF"
     gene            12326..12724
                     /locus_tag="VF226_12570"
     CDS             12326..12724
                     /locus_tag="VF226_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074868.1"
                     /translation="MTDFLEAAMLTAFSVSWYCSIWKMLTTGRASGKSLGFVLLICAG
                     YVCGIASKIVVFAETGAVSGLVLLYAWNLLVTGFDAFLVWHYGRGAEGAPQHALFPRL
                     AEAANKVFARSTSRAEAGAGSTPGRAAAVK"
     gene            complement(12735..13640)
                     /locus_tag="VF226_12575"
     CDS             complement(12735..13640)
                     /locus_tag="VF226_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX7074869.1"
                     /translation="MSELDVIMPFEAGDRLPVVRKIRVSDVGLALASGFADFWAMPTH
                     VVFLGLIYPVIGLLLAFAVFGLDMMVLLYPLAAGFALLGPFAAIGMYELSRRREMGLD
                     TSWRHAFDVVHSPSLRSIVTLGLLLLLWFVIWIWAANEIYIANFGYHKFESPSAFLAA
                     LLYTPEGHNVIIVGNIVGALFAIVAFATSVISFPLLLDRNVGVAVAVATSLKAVVRNP
                     IPMACWAAIIALGLLIGSLPLLVGLAIVMPVLGHASWHLYRRTVEAPEGQRPIYTPPD
                     KGRRYAAEFPASLFARSRDRESFDR"
     gene            complement(14165..14250)
                     /locus_tag="VF226_12580"
     tRNA            complement(14165..14250)
                     /locus_tag="VF226_12580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14214..14216),aa:Leu,seq:gag)
     gene            14494..15282
                     /gene="lipB"
                     /locus_tag="VF226_12585"
     CDS             14494..15282
                     /gene="lipB"
                     /locus_tag="VF226_12585"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7074870.1"
                     /translation="MSAHDLTPADWAISTEPVDYADAVAVMEARVAAIAAGKAPELVW
                     LLEHPPLYTAGTSTRWQDLIDPDRFPVFNAGRGGQLTYHGPGQRVVYTMLDVRRRTGD
                     VRAFVHALENWVIESLGTFGVLGETRADRVGVWVRRPERGEGVEDKIAAIGIRLRRWI
                     SFHGLSLNVSPDLTHFDGIVPCGVRQHGVTSLADLGHAIAMDEVDAALAEVFQRRMGA
                     IRRVSPPVADAAAAARLRALLTVERRRIKEKKPEAEAGSLASSS"
     gene            complement(15298..15636)
                     /locus_tag="VF226_12590"
     CDS             complement(15298..15636)
                     /locus_tag="VF226_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074871.1"
                     /translation="MSRITALVVGVAAVLASAEAAATVSLTNRDDRTYKVTIVENGTS
                     QDHSLAPSAVLEGICPHGCIIRLNDSEDDEYKLEGDEIVSIEDGFVYYDGSEPPEQPV
                     PGEGGDAHEK"
     gene            15971..16819
                     /locus_tag="VF226_12595"
     CDS             15971..16819
                     /locus_tag="VF226_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7074872.1"
                     /translation="MDINLARTFLTVAETGSFIEAARKMHVTQSTVSARVKVLEDLLG
                     RPLFQRSKYGASLTAAGEQFRKHALALVRVWQHAQLEVGLSDQHRDHLSVGGPLSLWE
                     GFLLRWVAWLRSSIPDIAISTTAGLSSALTQRLLEGTLDLAVLYRPVQPPGLVVEHLF
                     DEEFVLVTSGKSSSRRNNADYVFVNWGPDFQADHASAHPELATPGLNMDLGPLALSYV
                     LGNAASGYFPMRIVKPHLARGRLRLPKRVRKFVYPVYMVYPEARDEEAYEPILSALRR
                     EAAKLN"
     gene            complement(16884..17180)
                     /locus_tag="VF226_12600"
     CDS             complement(16884..17180)
                     /locus_tag="VF226_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX7074873.1"
                     /translation="MTRPASPVRAVHVRIEGRVQGVGFRAWVEDEATRLGLDGWVRNR
                     RDGTVEAVFAGDADRVTEMLARCEQGPPSARVARVEVVAEDVQVDPGFRVERTV"
     gene            complement(17191..18777)
                     /gene="ggt"
                     /locus_tag="VF226_12605"
     CDS             complement(17191..18777)
                     /gene="ggt"
                     /locus_tag="VF226_12605"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7074874.1"
                     /translation="MRSFHMPGRSPVYARRAMCATSHPLATQAAIEIMREGGNAVDAA
                     LTAAAVLAVVEHPMTGIGGDCFALISKPGHKLIALNASGRAPAAATADWYASKGITAI
                     EMQSPHAVTVPGAIDGWTTLLADYGSKPLSRILAPAIEYAEGGFPVAPRVAHDWANVS
                     EKLQGHPGSRQHLLPNGRPPRVGDVMRFPALARTLKIIADEGRDGFYQGEIAEDIVAE
                     LKELGGLHTLEDFAAQRCSYVEPISVAYKGVELWELPPNNQGIVALILLKILEETGFG
                     TDNATSAERYHMMMEAGRLAYAMRDAFVADPELAGVPVEHMLSDAVISDLAKRIDPKR
                     RTPDLGPVPRPSGSDTIYLSVADEQGMAVSFINSIYSPFGSGIMTRKTGIMLHNRGQG
                     FVLDPEHPNCIAPRKRPLHTLVPAIAMRNGKLFLSFGVMGAAFQPMGHAYVLTNMLDF
                     GMDPQEAIDCARVFFEGEELQVEESVPSATVERLASMGHKVTVRPEPWGGGQAICFDH
                     ENGVLIGASDARKDGCALGY"
     gene            complement(18882..19388)
                     /locus_tag="VF226_12610"
     CDS             complement(18882..19388)
                     /locus_tag="VF226_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HEX7074875.1"
                     /translation="MTIERRIADLFRMDEETWARHANPWSVWTRVVALPLLMLAVWSR
                     LWIGWWALLPTAAMILWLWINPRAFPPPRSTDNWASKGVLGERVWLNRREVPVPEHHR
                     LVPNILSASSALGAVLLIWGLVWLEIWPTLLGGAIVMLCKLWFVDRMVWLFEDMKDRH
                     PPYREWLY"
     gene            complement(19423..21444)
                     /locus_tag="VF226_12615"
     CDS             complement(19423..21444)
                     /locus_tag="VF226_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX7074876.1"
                     /translation="MFKKILIANRGEIACRIIKTARRMGIKTIAVYSDADPDALHVEM
                     ADEAVHIGPAPAAQSYLVIENIIEACKKTGAEAVHPGYGFLSEREAFPLALAEAGIVF
                     IGPNPKAIAAMGDKIESKRAAAKAKVSTVPGYLGEIADSAEAARIAEEIGYPVMIKAS
                     AGGGGKGMRIANSRKELEEGFDAARSEAKASFGDDRVFIEKFIVNPRHIEIQVLGDKH
                     GNVIHLGERECSIQRRNQKVIEEAPSPLLDEKTRKAMGEQAVALAKAVNYDSAGTVEF
                     VAGQDRSFYFLEMNTRLQVEHPVTELVTGIDLVEQMIRIAAGEELKLRQKDVTFTGWA
                     VESRIYAEDPFRGFLPSIGRLVKYRPPKEGSGDGMAVRIDTGVAEGGEISMYYDPMIA
                     KLITHAPTREAAIELQAQALDAFYIEGIQHNIPFLTALMQHPRWQEGNLSTSFIQEEY
                     PTGFQARAPEGEELQILAAVAAAIDHLHNARRRKITGQMSGRSVRFADRRVVELGNGL
                     SQRVHVDGKLEGPVKVALLDAEGTPTDTLEIASKWWPGEPVWSGMVRDRDVSVQVRPI
                     LNGFELAYRGIRVPARVYTEREAELAALMPVKKLADTSKVLLCPMPGLVKAIHVVVGQ
                     EIKTGDALVIVEAMKMENLLRAERDGVVKAVLAKPGDTLAVDAVIMEFA"
     gene            complement(21539..23071)
                     /locus_tag="VF226_12620"
     CDS             complement(21539..23071)
                     /locus_tag="VF226_12620"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7074877.1"
                     /translation="MKDILGELQRRREVAKLGGGEARIAAQHKRGKLTARERIDLLLD
                     EGSFEEFDMYVEHRCTDFGMAKTKIPGDGVVTGWGTINGRVVYVFAKDFTVFGGSLSE
                     THANKIIKIQDMALKNRAPIIGLFDAGGARIQEGVAALGGYGEVFLRNVLASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVKDTSYMFVTGPDVVKTVTNETVTAEELGGAKVHT
                     TKSSIADHAYENDVEALLQMRRLMDFLPSNNRSGVPVLPTKDSADRVEPSLDTLIPDN
                     PNKPYDIKELILKVVDEGDFFEIQEAFAKNIVVGFARMEGRTVGIVANQPLVLAGVLD
                     SDASRKAARFVRFCDCFEIPIITFVDVPGFLPGTAQEYGGLIKHGAKLLFAFAEATVP
                     KVTVITRKAYGGAYDVMSSKHIRGDVNYAWPTAEIAVMGAKGAAEILYRSELGDPDKI
                     AARIKEYQDRFANPFVAAERGYIDEVIRPRNTRRRICRALNMLRNKTVENPWKKHDNI
                     PL"
     gene            complement(23313..23642)
                     /locus_tag="VF226_12625"
     CDS             complement(23313..23642)
                     /locus_tag="VF226_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074878.1"
                     /translation="MSDEMAVFGALGGAAFCLTAGVIGAKGILAAAHNDGQRQVLKQV
                     FTWGGIYAATLAGLLLLLAFGVLPRWVYALAALLWFGPLMPALAWTHRRLDLAEGPTV
                     VGTPAAV"
     gene            complement(23737..24519)
                     /locus_tag="VF226_12630"
     CDS             complement(23737..24519)
                     /locus_tag="VF226_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEX7074879.1"
                     /translation="MSETGKEKTIPGKETLTPPKPRRFYKTAEVSTLQPHESTIDSKQ
                     FQVLLDGRPLRTPKKTALAVPYRALAEAIAAEWNAQGEEIDPVSMPLTRFANSIIDGV
                     IGREADVRADILKYAASDLVCYRASGPAGLVQRQAAAWDRVLNWAREALRARFVLTEG
                     VMPVAQPASALERVAGALEPLDAYRLTSLHVMTTLTGSALLALALSRGALSVEEAWSA
                     AHVDEDWQIEQWGEDAEAAERRRYRWQEMQSARRLLGLVDMN"
     gene            complement(24516..25199)
                     /locus_tag="VF226_12635"
     CDS             complement(24516..25199)
                     /locus_tag="VF226_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX7074880.1"
                     /translation="MRLIIFDCDGTLVDSQHMIVAAMTRAFHANGLELPMRDRILSIV
                     GLSLPIAIARLLPANADKGLVDQVAEAYREGFFELRQNPCYQEPMFPGALEAIQELAA
                     RRDVLLGVATGKSRRGVLALFERFGIASHFVTIQTADTNPSKPHPGMILEAIAEAGVE
                     PEEAVMVGDTTYDIEMARAANVGAVGVSWGYHPAVALEAAGAHLIVERCQDLVGAIDK
                     RFGASGVMA"
     gene            25680..26180
                     /gene="mraZ"
                     /locus_tag="VF226_12640"
     CDS             25680..26180
                     /gene="mraZ"
                     /locus_tag="VF226_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7074881.1"
                     /translation="MDRFVSTFTNKIDAKGRVSIPASFRAVLERDGYAGGIYCYPSLD
                     SPALDAGGERLAKKIDDLLAHLPDYSDERDELSVALYGDVQILSIDQDGRIVLPESLR
                     AHAGITNQVTFVGLGDKFQMWEPERFAERRQRARDKVHEHRKLFGAGSRRDNDEGQGG
                     GTGARE"
     gene            26180..27217
                     /gene="rsmH"
                     /locus_tag="VF226_12645"
     CDS             26180..27217
                     /gene="rsmH"
                     /locus_tag="VF226_12645"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7074882.1"
                     /translation="MAGRGSQGGLSTAREEGKDRSRHTPVLLIPVLKHLEPKDGATYV
                     DATFGAGGYTRAILDAASCRVLAIDRDPGAVAGGADLVAAYHGRLEVRQGRFSELEEI
                     AKDAGLTGVDGIVFDLGVSSMQLDEADRGFSFQADGPLDMRMSREGPTAADLVNTLDE
                     TELADILFHFGEERKARAIARAIVRARKQEPLTRTLQLARVVSQVLGGRKIDGRHPAT
                     RTFQALRIAVNDELGEVVAGLAAAERLLKPGGRLVVVTFHSLEDRIVKRFLAERAGKT
                     AGTSRHLPQAAQHRAESFRIVNQRPVTPDEEEIAANPRARSAKLRAGERTDAPSWPLD
                     PAALGVPQITR"
     gene            27243..27548
                     /locus_tag="VF226_12650"
     CDS             27243..27548
                     /locus_tag="VF226_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEX7074883.1"
                     /translation="MLRISSAIAVGLALISAFVLYAVSYETRHLDTEVAAKERRIERL
                     RSDIAVLKAERAFLSRPERIEPLARAMGFVPAEGSQYVELDSLKQSDAPPGQRPSSR"
     gene            27561..>29080
                     /locus_tag="VF226_12655"
     CDS             27561..>29080
                     /locus_tag="VF226_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX7074884.1"
                     /translation="MSTALGQPVGPPPSSAGINYAARAFAVALAIVVGFSAISLQLVR
                     LALRGQLDEPRVAMTQPLTESYWRPDIVDRNGRLLATDIEAPTLYADPALIIDVDEVV
                     EHLVQVFPEFDAVELRRSLSDRNRRYVRLKRGISPATAQRIHNFGLPGIAFRNEPKRV
                     YPNGALAGHVLGYVNIDNRGTSGLERFIDEAVGIEAVHSGAPGSLKPVRISLDLAVQY
                     GLEDELRAATRQYRAKGAAGVILDVNTGEVLAAASLPQVDPSDRSHILDASRLDKMTD
                     GVYELGSIFKMLTVAMALEAGTADLDKVYDVRVPLRVGRYTIRDFHPLGRPLSVREIF
                     IHSSNVGAGMMALELGSERQRAYLAKFGLIEPIRTEAGQVAPPKLPQNWGKAETITIS
                     YGHGMAVAPLQFAATAAALINGGWQVRPTFRSDPITTDRAQVISPETSAAIRELMRRN
                     VSSRHGTGRRADVPGYEVGGKTGTAELPGRGGYRETAVIASFVAAFPMSAPKYLVLV"
BASE COUNT         5420 a         9508 c         8896 g         5256 t
ORIGIN      
        1 gctcggtcat tcggatacag ggcgccggcg ggcagcaagg cggacaaagg ggcggcggag
       61 gcggtggggg tatgcaaagc ggccagggcg gtggtggagg caccatggag aacaggggtg
      121 gcggaggcgg aatggccgag ccccggggtg gtggcggagg caccatcgaa aaaagaggcg
      181 gcggcggcag tatgtcgaga ggcggccgcg ggacctggga gggccagagg cgcggtagag
      241 gcggcggaac cttggaacgt cggagaggcg gcggcggtgg catcatattg gaagagaggc
      301 ggagaccgcg cggctatcgc ggcccgcgct tccgtgaggg tcccacgctc agattccgga
      361 cgtcgtgcgg ctggctgaga gaacgggccc gtgataccgg cagccgctac tggtggcgcc
      421 gctatcgtga ttgcgtgcgt ggctgatcac gcgaaaagcg ggcgtcgcga cctcgcctgg
      481 gccagcttcg gcggctacgg cgagggctac tgagaacatt tgcgcagcga gccagcctat
      541 cggctcgctg ttggaggaat catcttgctg gtgcgatccg agggagcaag gaggttccca
      601 cgaggagatg ttgggatcac tctcctgcgc cttcgcgcct ctgtcgatca agtccggggg
      661 taggctcagt ttcaaaagag aaataaacgc ggaaggtggg gccgccgcat cggaagctcc
      721 aagccgttcg cgtggaaggc gtgcgtcatc gccgccacca tgatgtgctg actgtcgacg
      781 agagcccgtc gcagccggag atgtgagtat atgtggtgga tggcgtgcgg aacccagcgg
      841 atgtcctccc gaagtgctgt tgcgagccga tcacaaccct ccgccaagac caatctgttt
      901 cagtcgagga tgcctgccgc gtgactacct agatggtccg ctatcatctt tctggccgct
      961 cgagccccac ccaaactcat tcccatgaac tgcttctgga aatgcagctg ggcctgtaag
     1021 caaggggtct gatcaaggct tcggccaagc tgctcccatt tcaggaatcc attcagatca
     1081 aagttaaatc cgtgtggatc acggaatttc ggttcccaaa atacactatt ctcgttgctc
     1141 tcggtaaggt aattatcaat cagagcggcc gccatctcct caagctgact gccggcttca
     1201 tttcgatgct ccaagagcgc aatcactgca tccgtgatat ttgacaatcc agagggcaac
     1261 ccttccttct gcaggcaatc tctcaacaaa ttaatgaaca cgaaataata gagaaacttg
     1321 gtttgtcgtc gcgccgtatc accgccgcgc ccaaagcctt tctgttttcc agattcccac
     1381 agcaaaacgc agcatacaaa tcacgcgctc cgaatggcct ctcccctggg cgctcggacg
     1441 tcattctggt gaaaacactc ccgttcggca gaaacggtgg gttcttccca aacgcgagtc
     1501 ccggttcagc tagccaacca gcaccataga ctttcgtcaa gttgagtgca ctcgcaaaat
     1561 gcgtaaactg ccgggtcgtc gggttctgcc tctgaaacgc tcgttgggaa tcccaccctc
     1621 cgcgctgaat ttccaaatac aagccatatc tgtctgctaa ctccaggtgc cacgctttga
     1681 agccttggtt gattgcaacg aaatcacgct cacgcactgc attttggctg ttcgtaaatt
     1741 gcgttatctg ttggagcaga tcgtcccctt cgctgccaac tttcgcgatc tttgctacaa
     1801 cacacccttt ttcaactcgc gctcgccatg attcgagctt ctcgttcttg ccacttccac
     1861 cggcatccaa tctggaagac agcacctcgt ggattgacct tgtcgtttga catccattaa
     1921 cgacatatgg ctcaatcaac tcatacttgc cggcccccaa tatcttgaaa tctgacacag
     1981 ttatcgtgat tccattgttg aacagaccaa acttctcagg cgactctcgt aatgtggcgt
     2041 atataccttt attgactttc acgcgcccgc cgagaaacct acgtacattc ttctcataca
     2101 gttgatcaat agctcccgtc cgcccctggt acgatttcaa gaatgcatat aaatcgatca
     2161 atgacactga acccacaagc aggtcggtgc ccgcctgagt caaatgccca tgcagctcaa
     2221 aagtgaggtg ccgccggttt gcgagttcac tctcctcggc ctgtgcgtcg taaatggtgc
     2281 gaatcgatat ggcggccacg tcgaataggg cgccaagcct gtgacgcccc atggctctga
     2341 catcctcaag cgtcctttgc tcgtcaggtg ttaatggatt tattgtggcg taaactaaac
     2401 aaatgcggtc gtgctctcct gcagaatctc gaaaattcgt taacttctct cgaagtccgc
     2461 tggccagaca tgatagcttc gtcctactcc cgtccaatgt ctctatgact tttcttccct
     2521 ctactagcaa agttccttcg ccagcgaatg cggaaccata tttgctttga acaagatacc
     2581 aagtatctcc ttcgggcacg tcgtcgggac caggagggcc cccgcgatcg agtacagcaa
     2641 cgtcgatgcc gccatccccc gaaccgtcac aatagattac gtcaagggtg tcatcatcaa
     2701 tgtcgagcca ttgacttaca attttatggg caaagcggcg gccaacttcc acgctactag
     2761 gagcgccagc ggttatggtc tccaaccagc ttgtcttgaa gtcatcaaaa ctgaccgtgc
     2821 ttcccatgcg caccctcaat tcagcctaat caatctacgt tcagcatcgc tttgaaaaca
     2881 tgaccgccca cagcagagaa cagctgagag ttcctcgcct gtcaaggcaa tcagggtcgt
     2941 ctgtcagact actgaggaaa tttaacctcg aaacgtccag gtgacgagca cggaggggta
     3001 gtagccgagc tgaaaaaaga acgcttttac ttggtggtcg cgcatgagtt gcagcgcgaa
     3061 tatgcgcgct ggatacgact tgctgcaatt gatccgcgaa agcggaaacc acggggcttg
     3121 agttccaccg caaagccgcc gcctgcggcg agagccgttc ttcgcccgcg ccccaaactt
     3181 atccccgccc accaaccctg agtcataatc ccctcatccc ttccgcgagg ggacgttctc
     3241 cggaggcgtc cgtgaacggg gacggatgcg gtctcgaggg agatcgggcg cgagcctgac
     3301 ttggcgcgct cctatctaaa tcggaaatgc tctagccgcg cggggagcgc ttccagccag
     3361 cggcgatcgc ctcctcctcg ctgcagaacc accgttcgcc gcgccgggtc tcgatccgga
     3421 cacgacgata ctcgggcgtt ccgggtagca cgtaatattt cctgcccctg atgatgttgc
     3481 ccttgatggg gcatccgcgc ggtgcacggc gcttcgccgc ttcccaggcg cgcgccaact
     3541 ccttctcgac ccgcgcgcgg tattcggccg gccgctccgg gttcccacgc cagatcccgg
     3601 ctttttctgc tcgggccttt tcctcttccg catcatactt tgcgaacaga cccgtctcgg
     3661 cgaagacgtg tcctccggcg acgagcatcg acgcgatgtc gtccccgccg gcgacacagg
     3721 tcccaaggga tcgtcccttt tcgtcggtcc ccgatacgga gcaactgacg gacttgccgc
     3781 gaatcatgcg cgacagcgcg attcgggccg ccgctccgca gcgccagctc cggttgcccg
     3841 ggatggcgca gcgctgcgcc ggttccggcg cctctatgcc ggcaagcttg atttccctcc
     3901 cgccgatcct gagcacgtct cccgcaaccg cctcggcccg cccttcgatt ttctcggcga
     3961 acaggccagt ggagcttgcc gcgctgacca cgtgggaagc acccgtccag acgagccatc
     4021 ctccgccgat gagcgccgag caagcgacca gcacggcagc cgccaatcca gcggcgcggc
     4081 ccgtctgcgc aggcaacggc acacggaatc gatagggctt gcgctgttcc tggggcagca
     4141 gtgcgagatc ataacccgac gtgctgagcg atacagtgga gtcgccgagc attcgtacca
     4201 ggagggcgcc aatcagacat gcaccgccaa tgacgagcgc aaagacggcg tctgccgaag
     4261 tgccgctcgt cgccagacgg ataatcgctg cgaccgaagc caccacaccg acgagcacca
     4321 tcagcggcaa cgcagtcgag tgccgcagcg acttacagcc gatgacgagc agccgaaacg
     4381 acgtctgccc tattgcatca acacctatcc ccaatattcg cgccgcggca tacgcgaact
     4441 tgttcaggcg acggccgaaa tccatcagcg cttcgaaggc aaggatgagc gcgcggcggg
     4501 aaaagtcgag caagaccagc agcgctttcg cggagccctc ggcagcggcc gtcagggcac
     4561 gcgtcaaacc ggtgcgaatc gcttccccga tgattgggag aagcgagatc actaaggcca
     4621 ccccctcgcg cgccgcttcg ccaagcctcc ttgcgcattg tccgaggaaa ttcagaccaa
     4681 aggtgccggc tgccataccc gcacggcccg cactgctcaa gccttcggca gcgagagccc
     4741 gggcctcatc gatgcgccgc cgacggtcct cgcgcctgat cctgatggtc gtgcgcacat
     4801 gctcgtgcca ctcgaagccg tccgattttc ttcgccagcc gaacatctca tccccactgc
     4861 gcagaggcgc gggtccaagg cgaaactgga acctgcctcc cctcagacga ttcgtggagc
     4921 cgagtttgcc ggaaccgttc gggagtgccg agtcccggaa gcccgggcct gtgcaagacc
     4981 gctcccgtat cggtcaccgt ctgtcgcgca gtctgctcac gttccacagg gtcacggagc
     5041 cgctgtcgct gccggcggcc agccatcggc catcgggcga gaaagccacg gaccggtagg
     5101 cggccgaccc gtcgcccagg ctccgcagca agcgcccgcg ccggatgctc cagatcctca
     5161 cccggccatc cgcacttgct gccgcgagga gactccccgc gggagagaag gcaagtccat
     5221 gcaccacctc cgccctgcgg ctgaggaccc gataaaggcg gccggaggag gtcctcgaca
     5281 gccggatctg cccgtcgtgt ccggccgagg caagcagctt gccgtccggt gaaaaggcga
     5341 tggcgctgac gaagtcccga tggccgcgga aagtgcggat gagctcacct gatgaagcat
     5401 cccacagctt gaccagccgg tcggcgccgc ctgaggcgag caagccgccg ttgggggaaa
     5461 aggccacatc ctgcacggca ctcttgtgcg cctcgatcgt gactgcgggc gcctggaaac
     5521 gcgcatccca gacggccacg gtccaatcgt ggccggaggc agcaaagtgt tcagcgtcgc
     5581 ccatgaaggt gaccgaccag atactcgcgg aattccgctg gaattgcgcc agccgttcgc
     5641 cgtgcgccaa gtcccagagc tgaagcgtac cgtcctctcg ccccgtcaca gcgcgcgacc
     5701 cgcggacggc caaagcggtc accctgctct cgccgagacc gatcgtccgc tcattgctgc
     5761 cggttgcgac atcccagatc ttcaacgtcc gatcctcgcc ggccgtcacg atccggcggc
     5821 cgtcgtccgt gaaggcgacg gcgctcgttc cgccgctgtg gcccgcgatc tggcgcggct
     5881 gatcgatgtc ggaagcagtg cccgtccgag ttgcatcggc gctcatgaaa aacccgggca
     5941 ggcgatcctg cagtccaact gcgaaggctg ccacaccaag accgatcatc agcttcagga
     6001 tgagcggtcg ccacctccaa ccgccgaatg gtcggatccg tggcaatcga ggcagggaaa
     6061 aacgcggcag tcgaggcagc tgcggccact tccaaggctg cctcgccctc gcaggtttcg
     6121 caggccgatt tctggcgcgc ggcttttctg ccggggaggg cagatcgacc ttcggtgcag
     6181 gcttgttcgg ctgctctgcc gatttgggct gctccaccgc agcggtcggc gctgaggcgg
     6241 tcgccgctgg ggacgactgc ctcctcagcc gctcgatgta ctcgatcagt ccacccttgg
     6301 cgccaggagc atcgggtggc ggtggagcgg ctgtatcgag cttgagtgtc ttagccggtg
     6361 gatcctcctc agcagcgacc ttgccgatgg tccgctgcac ccagccggcc ttgcgcggct
     6421 ccggcgccag cagtggccca cgccactcgg ggatggactg cggcctcttc tttatgtcga
     6481 gcgacagcgc ctggtcgatg gctcgcagga acccgggccg ataggagctc agcgccgcct
     6541 cacgcgccgg cacgagctcg tccttgacga tgcgtgatgg ggcgtcgggc ggcctctttc
     6601 ccgttatggc gtgatagagc gtcgccgcga gcgcatagat gtccgtccac ggcccttgct
     6661 ggctgcccgt ctcggcgtac tgctcgtaag ggctgtaacc cggcttcacc aatgcgctga
     6721 ccgtccgcga atggcgcgcg atgtcaccgc gcgccgagcc gaagtcgatc agcaccggcg
     6781 cgccgtcgcg gcggatgatg atgttgtccg gcgcgatgtc gcgatgcaag aagttggcgt
     6841 tgtgaatgac ttcgagggca tcgagcagcg gcgctacgat ttggtccagc tcgaactgcc
     6901 gcggtgcccg cccgagactc tggagccagc ccttgaggct ctggccctcc tcgaagtgaa
     6961 gcaccatgta gccggtgttg ttcgcccgga aataacggta aacgcggacg atgttgcggt
     7021 gatcgaacat cgccagcgtc tgtgcctcgg cgatgaagcg gtcgaggccc cactgataat
     7081 cgccttccga ctcgcgggag cggggcactg cctcattgct gccgttccgt gctgcaaagt
     7141 cacccggaaa gtattccttg atggtgacct cgcgcccgag ggccagctcc tccgccaggt
     7201 atgtgatgcc gaaaccaccg gcgccgagga cgcggtcgat ccggtattct ccgacgagct
     7261 cggtaccggc gggaagcgcc agaaggtggg tcatgcgtct cgcttgcggc cgggcggtcg
     7321 caccatgcct cgcaacgaac cgccaccccc gcccctgcaa agcatttggg gtaggtggtg
     7381 gacggacaac atgtcacgaa tatggctgca aagtcggatg ggcggacgaa aggtgaggca
     7441 atcctcagtt cggacacatt ggctcgctgc catctcctgg tcagttcgag gcatcgaggt
     7501 tacgcaacgg taactgtccc ccggaatccg caccccgaag ggttgcaggg cgcaagcgct
     7561 tgcccgtata gttaggaccc gaggggaaac ggggtgatct ggagaattcg aatgcgttcg
     7621 accgccgcta tcgggttggc ggccctgttg ttttgctttt ggacacactc cgttgcccaa
     7681 gcgcaaccgg ctcaatgcaa agcaggtcct gccgcaatcg gcgtttctcg aaccgtcgag
     7741 atcgatgcgt cgacgggtcc acgctttggg cgctcccagt acagggattt cgacttcctg
     7801 caggatggag aggtcgttct gacgttcgac gacggaccat cgcggcgcca taccccggcc
     7861 attctcgatg cgctcgacgc ccattgcacg aaggccacct ttttcagtgt cggccggatg
     7921 gccgtggcag acccggaaat gctgcaggaa acagcgatgc ggggccatac catcggcacg
     7981 cacacgtggt cccacaagaa cctgcgttcg ctttccctgg cccaggcaag ggcggaggtc
     8041 gagcttggct tgagcgccgt gcaggccgct ctcggcggcc cggtggcgcc cttcttccga
     8101 ttcccctatt tgagcgatcc gcaaccggtt caggcgatgc tccagcagcg gggaatcgcc
     8161 accttttcca tagacgccga ttcctacgac tttcgcacct cgagtccacg gatggtgcag
     8221 gagaacatcc tcaatcagct tgcgcagcgt aagaagggta ttctgctgtt ccacgacatc
     8281 cagccttcga cggcacgcgc gttgaagggt ctgctcgatg agctcaaggc gcgcggctac
     8341 cgggtcgtcc acatcgtgcc gaaagcccct gcgacaacgc tggcggactt cgacgaaatg
     8401 gccgagcagg tgctcaggaa aaagcaggtc gctgcatcgg agcacccgct ggcaaggcac
     8461 cctgcgagcg ccccgcttgc gcattcatcg ggtgcgagcc gcccccgccc atcacattcc
     8521 gcgccgcgcc agaaccacac ccgccagcgc tatagggatg acaggtcctg gatagagcga
     8581 gtattccaga actgagcgcc cttaaccggc cctgcctcct gacggcaata cgccgggggc
     8641 gcaggccgcc acctcaaccg gcacgatcga tgacccgaac cggcctgcga acagcaagac
     8701 tggtgtccgc aggacgttct cgcggtggtc ctcgtaccga aacaatcgcg cgttgatgta
     8761 gtacacgaac aacaacgtcg cgcgggcccg cgccttcgag gcggactgaa cgaccgccaa
     8821 cgcctcccgt gacggctcat cgtggcgggc gaacatgatg tccatgaacg acttcaattc
     8881 cggccggccc tggtagagct gggcgagcgt cgcgtccaag ccggcacggt caccggcagc
     8941 cagctgcgac tgcgccttca tgaccagcag atagagccag aattgcgagt tgggctcgct
     9001 cagctccagc gcctgctcga gcgagctgag gccctggtcg atctgcccct cgaacgaccg
     9061 catctgtccg atgatggcga gggaggctgc gggagatgtg ctcgaccggt gggcattctc
     9121 ggcgagctcg acggccatcc ggcggtatcc gcggtcgatg aaatgcagca acttggccgc
     9181 catgatggcg taatacggct ccgcttgaat gaacggaagg ctggccgtga caagcgcctc
     9241 gatctcatcc tcgtcggccg agcggcagtc caggcccgcc atgaggatgt caatgccgtt
     9301 ctcgatgtat ttggtgtgga tgctggtcgc cagcatcagc ttggcgatgt gatcgttggg
     9361 atcctcggcg agcacctgcc gcaacctgcg ctcgctctcc tgccagctga tatgacctcc
     9421 gacgagagtt gcagaggccc tgtggagctg cacgggcagt ggctcgtgat gctccacatg
     9481 ggctgcgagg ttccgccata tcgaggatgc tatacggtcg gcgagagcgt ccgccgtcgc
     9541 gagctggcct gcgacttgat cctccgcggt cgcaatccgg tggcgttcga cgcaaaagag
     9601 cctcccagtg cggaatgccc tcgcccgaaa gacgcaatcg agtccaacac cgtcgcaaag
     9661 aaatgtgaga tcgacagcga tttccggaca gtcgtcgccg aaggagctgc gcggcgggca
     9721 atccggatcg agcacgacct ttcggtcagg gctgaaatgc ctgcccagcc gcagctcgaa
     9781 ggtccgtgcg aagccaatcg caaacgattt gaatgctcca atgtgactga ggccacggag
     9841 cggtccgacc acaacgtaag cgccgcttgc gagcggtcgc ccactcagcg cctttgcaac
     9901 ccagatgtac ccttcaccat accgggtggc aatgaacctc ggattcttcg ggtcgtcatt
     9961 cagcttccta cggagccggt tgatggtgaa gtcgatattg cggtcgcttt tgtctgagcc
    10021 tggttccgag atcgcatcaa gcaactgatt tcgcgaaagg actcgcccgg cattgcggac
    10081 catgtgagcg agcagtcgac tttccgaacg ggtgaaccga acttcgagcc catcgtcgcg
    10141 gagcgcggac tggaaggcat cgtcgaaaga ggcggagccg agccggagaa gccgagtctt
    10201 gtcgttcatg aagagaatga gcccatttac tacaataggt tacagtattc cgcgatcgca
    10261 acctaccccg cgagtgctgc ccaaaaccga ccggccgatt ggtgcaggca gaattacccg
    10321 cctaaaaatc aaatattcct gcaacaattt acttttaacg tatctttcgt cagaggcgag
    10381 tcaatcccgc ttcggatccg ctcacatttt ttgagcggtc gtgtcgaact gccgaggcca
    10441 cgtgtcgtcc aaagcatctc gatgcgagtt tttcccggcc ctgatgcggg cctgaattgc
    10501 gcaatcctag cttccgcggc tgcagtcgct ttcacgaaag cctctgcacc gcggtttttt
    10561 ccgacagaaa ctatcccaac cggcgctcaa aatcgcatag cagacctgac ccagagccgc
    10621 gttcccttct gctccacgat tccgtccggc tcgtcgaccg ttcgcgacag cgaagcttct
    10681 gcaatgaaat ttgacgtagc tgcgcggacg ccgagataag cgctggccca ggtgtcttgc
    10741 gacgcgggtg ccacgtcgga tctgatgttc cacagccagc cggcatctgc ggcggcgaag
    10801 agctgcagaa acttcagcag gccaccgctc aaatccgctg caggaagatt gtaggccatt
    10861 tcacctcgcc cgcccagaca ctcgtcccca ttgacgaagg cgcgatcgaa ggcacggctg
    10921 aactcgttcg ttccgtagcc gcagcgctgg gaaaagggca gcggcgcatc ggaatattgc
    10981 ccaatcgcac caatggtggc caccacctgc tcagtcagcg gaacagcgag gtcggcactg
    11041 aaggagacag tcaaaaaatc cgaggacgca ccaggcagcg aagccatcgg attatgagtg
    11101 tccgagctgc ccagtccggt acggcccttg tgcaggatca cgccgaaccg ctgccgtatc
    11161 ccgagaggcg tgcggccagc ataagagaac ctggcgctca gcacgcgcag atcctcgtcg
    11221 aggaccggcc ggtcgaaagc ttggcctttc cattgtctag ccgtgaagct caactcgccc
    11281 gtgagcttcg agccttgcga aacgacccag tcttgacggg cgccaacggt gatagtccag
    11341 ttttgacctg tcgcatctat cgcccggttt gcgggagcgc ccaacctgat gtccgcgtaa
    11401 tcgccgttca cgaacaatgt cgggccggag ttgggtagct tgatcctgta gccgagacct
    11461 ccggccagca gctcctgcct gccggtctcg aacggcccgc cgagcacggc gacagcgtcg
    11521 aactggtcac cttgcacgaa caaatccggc agggccgccg ccgtgctgag gatggaaggg
    11581 ccaatgtcct ctgatccgaa agtgtcgaag gtgatgaagt aggtgttctt ctcgaccgga
    11641 ccgatcagcg ccgtcgatcc acgctcgacg ccgatcgcct cccgaatggt cgtcgggacg
    11701 aagttcgaat ctgcgtgcgc gacttgcggg ctcagagcca gcgaaatgag gctcagcgcg
    11761 cgaggtgccc aaagtgggac accttggaat cttgtcaact tccccccttc tcgaacgctc
    11821 cgcccaagcg acagcgcaag caccgccggg gcgccggagg cgctctcact tttgaagacg
    11881 atttgcagat gagccgggac cggtcgttcg gctcgcgcgg tcctgcgccg cgccctggcg
    11941 tctcaagtgc tatcggcggt caatagggct cctccccaaa gccaacaagc tccgcccatc
    12001 cggccaacgg gactacttct cccgaggtca attgcggagc cacggtcaga atatgcagat
    12061 gacagatgaa aggcgaggat tttctcacga tgagcaccct ccttgccggc atgcccaggt
    12121 gacgtgcccc cggcccatga tgatcgctgg taacggtccg atcacctctt gaacccgact
    12181 ggccactcga cagggcgatc aaacaattct gcaacaatgt ctgccgaagt tgctccggtg
    12241 ctcgagctgc gcgccgcgtc atcggccatg agatccgaaa tgccggcgct caccagaagc
    12301 cctttccggg agcgatgcgc gtccaatgac cgactttctc gaagctgcaa tgctcaccgc
    12361 gttttcggtg agctggtact gctcgatctg gaaaatgctg accaccggcc gcgcttccgg
    12421 gaaaagtctc ggcttcgtgc tcctgatctg cgccggctac gtgtgcggaa ttgcctccaa
    12481 gatcgtggtg ttcgcggaaa cgggagccgt ctcgggcctc gtcctgcttt acgcctggaa
    12541 ccttctggtg acgggatttg atgcgttcct cgtctggcac tatggacgcg gcgccgaagg
    12601 cgcaccgcag cacgcccttt tccccagact ggcggaggcg gccaataaag tatttgctcg
    12661 cagtacctcg cgcgccgaag cgggagccgg gagcactccg ggccgtgcgg cagcggtgaa
    12721 gtgatcctgg cggctcaacg gtcgaagctc tcccgatcgc gcgagcgcgc gaaaaggctc
    12781 gctgggaact cggccgcata ccgccggccc ttgtcgggcg gagtgtatat gggccgctgg
    12841 ccttcgggtg cctctaccgt ccgccggtag agatgccagc tcgcgtggcc gagcacgggc
    12901 atcacgatgg cgagaccgac caacaacggc agagacccga tcagcaagcc caaagcaatg
    12961 atcgccgccc agcacgccat gggaattgga ttacgcacca ctgccttgag tgaggtcgca
    13021 acggcaaccg cgaccccgac attgcgatcg agcagaagcg gaaacgaaat gacactggtg
    13081 gcgaaagcca cgatggcgaa gagggcgccg acgatgttgc cgacgatgat gacgttatgg
    13141 ccttccggag tgtacagcaa cgctgccaaa aatgcgctcg gcgactcgaa tttgtgatag
    13201 ccgaaattcg caatgtagat ttcattcgcg gcccagatcc agatcacaaa ccagaggaga
    13261 agcaatagcc cgagcgtgac gatggaccgc aaagaggggg agtggacgac gtcgaaggca
    13321 tggcgccagg aggtatcgag acccatctcg cggcgccggc tcaactcgta catgccgatc
    13381 gcggcgaacg ggccaagcag ggcgaagccg gcagcgagcg ggtagagcag caccatcatg
    13441 tccaggccga aaaccgcaaa cgccagcagc agcccgatga cgggatagat cagaccgagg
    13501 aaaacgacgt gggtcggcat cgcccagaaa tcggcgaagc cgctcgccag cgccagcccg
    13561 acatcgctga ccctgatctt tcgcacgaca ggtaacctgt cgccggcttc gaagggcatg
    13621 atgacatcga gttcggacat gaccggtcct gctcgagagt gctcggcata agcgggcgaa
    13681 gccaccggcg ccgcactgcg ctgcccgaac gtcaggtcac cttggcgaca atcgcttcca
    13741 tcccgtcaga agaaacggcg tgcaccatct tggaagggca ggcggcccaa gcaccggcat
    13801 tcgcccaacc tccctttttg ggagccaagt cttcggtgct catagcaact taaaaactcc
    13861 caaccggcca gcatggttca cccctttccc gggcgatttc gctcggaatc acgttcgcca
    13921 ttctcggtgg cggcgtagtc tattcgctgc ccgtgacacg taggctggct gttgcccaga
    13981 gaaagaacct ggggcctgag gctggggctc agttcaacgg tggggccgat ttctgtccgg
    14041 cttgcacctg catggcccca acacctttcg tgcagatgcg gccagccaac cagagcgaca
    14101 gaacagctgt tcacgtcccg ctgtatcccc aaaaggggca atgtcttgtc tcgaggatat
    14161 gcaatggtgc ggtcgagagg actcgaacct ccacgccttt tgggcgctac cacctcaagg
    14221 tagtgcgtct accagttccg ccacgaccgc atgccaggga acgagcaccc gtcccttagc
    14281 aaactccccg gcggctcaca agcggtccgc cagaggccgc ctcacgtacc agaccctgcc
    14341 gtccggtgca agggaactcg tgcacctcat cgcgcccccg cgacgttgca gacgcctgct
    14401 gaaccagccg tgatgacacg ccatctgttc atcccgttgc aagtgggatt gaatggctgc
    14461 cctgggatat gaaggcgtag acaagccact atcatgagcg cgcacgattt aaccccggca
    14521 gactgggcca tcagcaccga accggtcgat tatgcagacg cggttgccgt gatggaggcg
    14581 cgggtcgcag cgatcgcggc cggtaaggcg cccgagctcg tctggctgct cgagcacccg
    14641 ccgctctata cggcagggac gagcacgcgc tggcaggacc tcatcgaccc ggaccgattc
    14701 ccggtcttca acgccgggcg cggcgggcag ctcacctacc acggcccggg ccagcgtgtg
    14761 gtctacacca tgctcgacgt ccgccgccgc accggcgacg tgcgcgcctt cgtccacgca
    14821 ctcgagaact gggtgatcga atcgctcggc accttcggcg tcctgggtga gacgcgcgcc
    14881 gaccgcgtcg gcgtgtgggt gcgccggccc gagcggggcg agggcgtcga ggacaagatc
    14941 gccgccatcg ggatcaggct ccgccgctgg atcagcttcc acgggctgag cctcaacgtc
    15001 tcacccgacc tgacccactt cgatggcatc gtcccttgcg gggttcgcca gcacggcgtg
    15061 acgagccttg ctgacctcgg gcacgcgatc gcaatggacg aggtggatgc tgcactggcg
    15121 gaagttttcc agaggaggat gggtgcgatc cgccgtgttt ctccgcccgt cgccgatgca
    15181 gctgccgccg cccggttgcg ggccctcctc actgtcgagc gtcgcaggat caaagagaag
    15241 aagcccgagg cggaagcagg atcgcttgcc tcgagctcct gaagccagaa ccgcagccta
    15301 cttctcgtgg gcgtcaccgc cttcacccgg aacaggttgc tccgggggtt ccgaaccgtc
    15361 gtagtacacg aagccgtcct cgatcgagac tatctcgtcg ccttccagct tgtactcatc
    15421 atcttcgctg tcgttgagac ggatgatgca gccgtgcggg caaatccctt cgaggacagc
    15481 cgatggcgcc aaggaatggt cctgactggt gccgttctcg acgatggtga ccttgtacgt
    15541 acgatcgtcg cggttcgtca gggacaccgt cgcggcagcc tccgcactgg ccaatactgc
    15601 cgcaacacct acgacgagag ctgtgatgcg cgacacgagt tgctccaatg actgcgagtc
    15661 gggcccgcgg cttacttctt cttctttgca ggagccttct tggcgggggc cttcgcagcc
    15721 gcctttttcg ccgcaggttt cgccgtcttt gccttcatct cgacgttcct tctctctatc
    15781 tcagtttctc cacccggttg agggcagaag actcgattct tcttcgagtc gcgagcaatg
    15841 tgggtagaat caaatgagtc tacaaacgga tttttccggt gacttctatc gaacatttcg
    15901 atagttcatc gaagcgggag aaacgaccag aacaggctgc aattgcacag ccgaggcgta
    15961 ttcgaggccc atggacatca atctggcccg gacgttcctg accgtcgccg agaccggcag
    16021 cttcatagaa gcggcgcgca agatgcacgt gacgcagtcg accgtcagtg cgagggtcaa
    16081 agtgctggag gatctgctcg gacgaccgct gttccagcgc tccaagtacg gtgcctcgct
    16141 gacggctgcc ggcgagcagt tccgcaagca tgcgttggca ctggtgagag tctggcagca
    16201 tgcacagctc gaggtcggcc tctccgatca gcaccgggat cacctctccg tcggggggcc
    16261 gctcagcctc tgggagggct ttctgctgcg ctgggttgcc tggctgcgca gcagcatccc
    16321 cgacatcgcc atttcgacga ccgctggcct ctcctctgcg ctcacgcagc ggctcctcga
    16381 aggcacgctc gacctcgccg ttctgtaccg gcccgtgcaa ccgccagggc tcgtggtcga
    16441 acatctgttc gatgaggagt tcgtgctcgt cacgagcggc aagtccagct ctcgccgcaa
    16501 caatgcggac tatgtcttcg tgaactgggg gcccgatttc caggccgacc acgcttcggc
    16561 ccatccggag ctggccaccc ctggcctcaa catggatctc gggccgctgg cgctttccta
    16621 cgtcctcggc aatgccgcct ccggctattt cccgatgcgc atcgtgaagc cgcacctcgc
    16681 acgcggccga ttgaggctgc cgaagcgggt gcgcaagttc gtctatcccg tctacatggt
    16741 ctatcccgaa gcgcgggacg aggaggctta cgagcccatc ctcagcgcgc tgcgccgaga
    16801 ggcggcgaag ctgaattgaa ccgagcttgg cggggatttc ggattttcgc cgctctccac
    16861 catcgcgcgt gagggcgaat tcgtcagacg gttcgctcga cccggaagcc tggatcgacc
    16921 tgcacgtcct cggcgaccac ctcgacgcgt gccacccgcg ccgacggcgg gccctgttcg
    16981 cagcgagcga gcatttcggt gacacgatcc gcatcgccgg cgaataccgc ctcaaccgtc
    17041 ccgtcgcggc ggttgcgcac ccagccgtcg aggcccaacc gcgtggcctc gtcctcgacc
    17101 cacgcccgga aaccaacccc ctgcacgcgg ccctctatgc ggacgtgaac ggctcggacc
    17161 ggtgaagcgg gcctcgtcat gcggcctccc tcagtagcct agcgcacatc cgtccttccg
    17221 cgcatcggag gcgccgatca gaacgccgtt ctcgtgatcg aagcaaatgg cctgaccgcc
    17281 tccccacggc tcgggccgca ccgtcacctt gtggcccatg ctggcgagcc tttccaccgt
    17341 tgcgctcgga accgactcct ccacctgcag ttcctcgccc tcgaagaaga cgcgggcgca
    17401 gtcgatcgcc tcctgcgggt ccatgccgaa gtcgagcatg ttcgtgagca cataggcgtg
    17461 ccccatgggc tggaacgcag cgcccatcac gccgaacgac aggaagagct tgccgttccg
    17521 catggctatc gccggcacca gggtgtgcag gggccgtttg cggggggcga tgcagttcgg
    17581 gtgctccgga tcaaggacga atccctgccc ccggttgtgc agcatgatgc ccgtctttcg
    17641 cgtcatgatg ccggagccga acgggctgta gatcgagttg atgaaggaaa ccgccatccc
    17701 ctgctcgtcg gccacgctga ggtagatcgt atccgacccc gacggccgcg gcaccgggcc
    17761 gaggtccggc gtccgccgct tgggatcgat gcgcttggcc agatcgctga tgacggcatc
    17821 cgacagcatg tgctcgacag gcacgccggc caactccggg tcggcgacga aggcatcgcg
    17881 catcgcgtag gcgagccgcc cggcctccat catcatgtga tagcgctcgg cgctggtcgc
    17941 gttatccgtg ccgaagccgg tttcctccaa gatcttgagc aggatcagcg cgacgatgcc
    18001 ctgattgttc ggcggcagtt cccacagctc gacgcccttg taagcgacgg agatcggctc
    18061 gacgtaggag caccgctggg ctgcgaagtc ctccaacgta tggagcccgc ccaattcctt
    18121 cagctcggcg acgatatcct cggcgatctc gccctgatag aagccatccc ggccctcgtc
    18181 ggcgatgatc ttcaacgtgc gcgcaagcgc cgggaaccgc atgacgtcgc ccacgcgcgg
    18241 tggccggccg ttcggcagga gatgctgccg cgagccggga tggccttgca gcttctccga
    18301 gacattcgcc caatcgtggg ccacgcgcgg ggccaccgga aacccgcctt ccgcatattc
    18361 gatggccggt gcgagaatgc gcgacagcgg cttcgatccg tagtctgcaa gcagcgtcgt
    18421 ccatccgtcg atcgcacccg gcaccgtgac agcatgcggc gactgcatct cgatcgccgt
    18481 gatgcccttg gaggcgtacc aatccgccgt cgccgcagcc ggcgcgcggc ccgaggcatt
    18541 gagcgcaatc agcttgtggc cgggcttcga aatgagagcg aagcaatcac cgccgatgcc
    18601 cgtcatcgga tgctcgacca ctgccaacac cgcggcggct gtgagcgctg cgtcaacggc
    18661 attcccgccc tcccgcataa tctcgatggc cgcctgcgtc gcgagcgggt gcgacgtcgc
    18721 gcacatcgcg cgccgggcgt acacaggcga gcggccgggc atgtggaagc tgcgcatgga
    18781 gttcctctga ttggttatgg gcgcagagat tgcgcgcagt ggagtgacgg ccagcggcct
    18841 tgctcatgtt gccgcagggg gagcgcaaac tgcaagtctc gtcagtagag ccattcccgg
    18901 tagggcgggt gccgatcctt catgtcctcg aacagccaga ccatccgatc gacaaaccac
    18961 agcttgcaaa gcatcacgat ggcgcccccg agcagcgtgg gccagatctc cagccagacc
    19021 aagccccaaa tgagcagcac cgcgcccaaa gccgaagagg cagacagaat gttcggtacc
    19081 agccgatgat gctccggcac cggaacctcc cgacggttca gccagacgcg ttcacccagc
    19141 acgcccttgg acgcccagtt gtcggtcgac cggggtggcg gaaacgcccg cggattgatc
    19201 caaagccaca ggatcattgc agcggtcggc agcagcgccc accagccgat ccagaggcgg
    19261 ctccacaccg ccagcatcaa gaggggcaga gcgacgaccc tggtccacac actccaggga
    19321 ttggcatggc gcgcccacgt ctcctcgtcc atgcggaaga ggtccgcgat gcggcgttcg
    19381 atggtcatgc cctctcacat ggcccctggc gcggcgtcag gatcacgcga actccatgat
    19441 aaccgcatcg accgcaagcg tgtctccagg ctttgcaagg accgccttga cgacgccgtc
    19501 gcgctcggcg cggagaagat tttccatctt catggcctct acgatcacca gtgcatcgcc
    19561 tgtcttgatc tcctggccga cgacgacgtg gatcgccttg acgagccccg gcatggggca
    19621 cagcaggacc ttcgaggtat cggccagctt cttcaccggc atcaacgccg ccagctccgc
    19681 ctcccgctcg gtgtagacac gcgccggcac gcgaatgccg cggtaagcca gctcgaagcc
    19741 gttgaggatc ggccgcacct ggaccgaaac gtcacgatca cggaccatgc ccgaccagac
    19801 cggctcaccc ggccaccatt tcgaggcgat ctccagggta tcggtcggtg tcccctcggc
    19861 atcgagcagc gccactttga ccggcccttc gagcttacca tccacgtgca cccgctgaga
    19921 aaggccattg cccaactcca cgacccggcg atcggcgaaa cggacgctgc ggccggacat
    19981 ctgccccgtg atcttccgcc ggcgcgcgtt gtgcaggtgg tcgatggccg ccgccacagc
    20041 cgccaggatc tgcagctcct cgccttccgg cgcgcgcgcc tgaaatccgg tgggatactc
    20101 ctcctgaatg aacgacgtcg acagattgcc ctcttgccag cgcggatgct gcatcagcgc
    20161 cgtcaggaac gggatgttgt gctggatgcc ctcgatatag aacgcatcga gcgcctgagc
    20221 ttgcaattcg atcgccgcct cgcgcgtcgg tgcgtgcgtg atgagcttgg cgatcatcgg
    20281 gtcgtagtac atggagattt cgccgccctc agccacgccg gtatcgatgc gcaccgccat
    20341 gccatcgcct gagccttctt tcggcggacg gtatttcacg aggcgcccga tcgacggcag
    20401 gaaaccgcgg aacggatctt cggcgtagat acggctctcc acggcccagc ctgtgaaggt
    20461 cacgtccttc tggcggagct tcagctcctc gccggcagcg atccggatca tctgctccac
    20521 gaggtcgatg ccggtgacga gctccgtgac cggatgctcg acctgcaggc gcgtgttcat
    20581 ctcgaggaag tagaagctcc gatcctgccc tgccacgaac tcgaccgtgc ctgccgagtc
    20641 gtagttcacc gccttggcca acgcgaccgc ctgctcaccc atcgccttgc gggtcttctc
    20701 gtcgagcagc ggcgacggcg cctcctcgat caccttctga ttgcggcgct ggatcgagca
    20761 ctcgcgctcg ccgaggtgga tgacgttgcc gtgcttgtcc cccagaacct ggatctcgat
    20821 gtgccgcggg ttgacgatga acttctcgat gaagacgcgg tcgtctccga agctcgcctt
    20881 cgcctcggag cgggcggcgt cgaacccctc ctccagctcc ttccggctgt tggcgatgcg
    20941 catgcccttg cccccgccgc cggcagaagc cttgatcatc acgggatagc cgatctcctc
    21001 ggcgatgcgc gccgcctcgg cggagtcggc gatctcgccg aggtagcctg gaaccgtcga
    21061 gaccttcgcc ttggccgcgg cgcgtttcga ctcgatcttg tcgcccatgg ccgcgatcgc
    21121 cttgggattg gggccgatga acacgatgcc cgcttccgcc agcgccagcg gaaaggcctc
    21181 ccgctccgac aggaagccgt agcccggatg caccgcctcg gccccggtct tcttgcaggc
    21241 ttcgatgatg ttttcgatca ccagatacga ctgcgcggcc ggcgccggcc cgatgtggac
    21301 ggcctcatcc gccatctcga catgcagcgc atccgggtcc gcgtccgagt aaacggcaat
    21361 cgtcttgatc cccattcgcc gggccgtctt gatgatccgg caggcgattt ccccgcggtt
    21421 ggcgatcaga atttttttga acatcgtcag tcaaggctcc cggtcgcctc cgcaacgacg
    21481 gaggcatgca ggctgggtga ggaaatggat tcgtcagcgc caggatcgcg agggggactc
    21541 acagcggaat attgtcatgc ttcttccagg ggttctcgac cgtcttgttg cgcagcatgt
    21601 tcagcgcgcg gcagatgcgg cggcgcgtgt tgcgcggccg gatcacctca tcgatatagc
    21661 cccgctcagc cgccacgaac gggttggcga agcggtcctg atattccttg atgcgggcgg
    21721 cgatcttgtc ggggtcgccg agctccgagc gatagaggat ctcggccgcg cccttggcgc
    21781 ccatcaccgc gatctcggcc gtcggccagg cgtagttgac gtcgccgcgg atgtgcttgg
    21841 agctcatcac gtcgtaggca ccgccgtagg ccttgcgcgt gatcaccgtc accttcggca
    21901 ccgtcgcctc cgcgaatgcg aaaagcagct tggcgccgtg cttgatgaga ccgccgtact
    21961 cctgcgccgt gccgggcagg aagccaggga cgtcgacgaa ggtgataatc ggaatttcga
    22021 agcagtcgca gaagcgcacg aaccgggccg ctttgcgcga cgcatccgaa tcgagcacgc
    22081 ctgccagcac cagcggctga ttggcgacga tgccgaccgt gcggccctcc atgcgggcga
    22141 agccgacgac gatgttcttc gcgaacgctt cctggatttc gaagaagtcg ccctcgtcca
    22201 cgaccttcag gatcagctcc ttgatgtcgt agggcttgtt cgggttgtcc gggatgagcg
    22261 tatcgagcga gggctccaca cggtcggcgc tgtccttcgt cggcagaacg ggaacgccgg
    22321 accgattatt cgacggcagg aagtccatga gccgccgcat ctgaagcagc gcctcgacgt
    22381 cgttctcgta ggcatggtcg gcaatggagg atttcgtcgt atggactttc gctccgccca
    22441 gctcctcggc cgtgacagtc tcgttggtca ccgtcttcac cacatcgggg ccggtgacga
    22501 acatgtagct cgtgtccttc accatgaaga tgaagtccgt catggcgggc gaatagacgt
    22561 cgccgcccgc gcacggcccc atgatcaccg agatctgcgg gatgacgccg gaggccagca
    22621 cgttgcgcag gaacacctcg ccatagccgc cgagcgccgc caccccctcc tgaatgcgcg
    22681 cgccgcccgc gtcgaacagg ccgatgatcg gcgcccggtt cttgagcgcc atgtcctgga
    22741 tcttgatgat cttgttggcg tgcgtctccg acagggagcc gccgaacacg gtgaaatcct
    22801 tggcgaacac gtagacgacg cggccgttga tggtgcccca gccggtcacc acgccgtcgc
    22861 ccgggatctt ggttttcgcc atgccgaagt cggtgcagcg gtgctcgaca tacatgtcga
    22921 actcctcgaa cgagccttcg tcgaggagca ggtcgatgcg ttcgcgggcc gtaagcttgc
    22981 cgcgtttgtg ctgtgccgcg atccgcgcct caccgccgcc gagctttgcc acctcgcggc
    23041 gccgctgcag ctctccgaga atgtccttca tggctggcag agacccttct gggcaggaga
    23101 tcaatcgtca ggcgcggtgt agcacgcggc gaacggggtg caaatctcgt gcgcggccgc
    23161 ccaggctgac ggcccgggtc cagatccggc gggctcaggt ccgtgcagaa aagtcgccga
    23221 aggagggtga cgcgactgat cgcgaaaatg gagcgccctc cacgggcgcc cgtctcgacg
    23281 caggaacaag aacagtgact gacggccagg cttcagaccg ccgcaggcgt gccgaccacc
    23341 gttggacctt ccgccagatc gagccgccga tgggtccagg cgagtgctgg catcagcggc
    23401 ccgaaccaca gcaatgctgc caaggcataa acccagcgcg gaagcacgcc gaaagccagc
    23461 agcaagagga ggcccgcaag ggtcgcggca tagatgccgc cccaggtgaa tacctgtttg
    23521 aggacttgcc tctggccatc attgtgtgca gccgcaagga tccccttggc accgatgaca
    23581 ccggcagtga ggcagaaagc agccccgccc aatgctccga aaacggccat ctcatcactc
    23641 atggcgcagc cccctacgct cacttcagct gtctgattat gccgcggtag ggtaaccacg
    23701 cgcttaacac gtgtcttcgc acggggcttt gtgactttaa ttcatgtcca ccaagccgag
    23761 caggcgcctt gcagactgca tctcctgcca gcgataacgg cgacgctcgg ccgcctcggc
    23821 gtcctcgccc cactgctcga tctgccagtc ctcatcgacg tgggccgctg accaggcctc
    23881 ctcgacggac aacgcacccc ggctcagggc aagagcgagc agcgccgagc ccgtcaacgt
    23941 cgtcatcacg tggaggctcg tgagccgata agcatcgagc ggctcgagag cgcccgcaac
    24001 cctttccaag gccgacgccg gctgtgccac cggcatcaca ccttccgtca aaacgaaacg
    24061 ggcgcgaagg gcctcgcgcg cccagttgag cacgcgatcc caagctgcgg cctgacgctg
    24121 gacaaggcct gcggggcccg aggcgcggta gcacaccaag tcgcttgcgg catattttaa
    24181 tatgtcggcc cgcacgtccg cctcgcggcc gatcacgccg tcgatgatgc tgttggcgaa
    24241 gcgggtcaga ggcatgctga cgggatcgat ctcctcgccc tgcgcattcc attccgctgc
    24301 gatcgcctcg gccaaggcgc gatacgggac ggcgagcgcc gtcttcttcg gcgtccggag
    24361 cgggcggcca tccaacagca cctggaattg cttcgagtcg atcgtagatt cgtgcggctg
    24421 tagggtggag acctccgccg ttttgtaaaa gcgccgcggc ttgggcggcg tcagggtctc
    24481 cttgcccgga attgtctttt ctttacctgt ctcgctcacg ccatcacccc agatgcgccg
    24541 aagcgcttgt cgatggcacc gaccagatcc tggcagcgct cgacgatcag gtgggccccg
    24601 gcggcctcca gtgcaaccgc cggatgatag ccccagctga cgccgacggc gccgacgttc
    24661 gccgcgcgcg ccatttcgat atcataggtg gtgtccccga ccatgacggc ctcttctggc
    24721 tccacgccgg cttcggcgat ggcctccagg atcattcccg ggtgcggttt cgacgggttc
    24781 gtgtctgccg tctggatcgt gacgaaatga gaggcgatcc cgaaccgctc gaacagcgcc
    24841 aagacgccgc gccgtgactt gcctgtagcg accccgagca ggacatcccg tctggcggcc
    24901 aactcttgga tggcctccag ggcgccggga aacatcggct cctgatagca ggggttctgg
    24961 cgcagctcga aaaacccctc gcggtaagcc tcggccactt ggtcgacgag gcccttgtcc
    25021 gcattcgccg gcaacagacg cgcgatggcg atcggcaacg agaggccgac gatggacagg
    25081 atccgatccc gcatcggcag ctcgagaccg ttggcgtgaa aggcccgcgt catggcggcc
    25141 acgatcatgt gctgactgtc gaccagcgta ccgtcgcagt cgaaaatgat gaggcgcatg
    25201 ggggtaccta tagcccccgc ggtggccggg gaccagagcc gcctgggtcg cgcctggagc
    25261 ggaaagcctc tccaggcgcg cggagcgcca gaagtccccc cgccctgcgg ggcggccgcc
    25321 ttacgcaaat ccgaaaaaag aagtgggacc agaaacccgg acgagtgtca ctcatggtcc
    25381 ggtttgactg tgactcaaga gccacaatta acctctctcc cagacctcgc catctcattc
    25441 gttaacatcc cgttgacgcc catacgattc catgcgatcc cataaatgcc cgtccgcacc
    25501 gggcatggga cagtggccgt ggcgccggct tcgttgaggg cccgaagccc cgagcgaccg
    25561 ccacgtccca gcccggcgga gacgggcccc gacctctacc gggagttgag cgcacggcgt
    25621 cacgagaggg gaggtattgg cgtcgaagtc tgcgcgtcag gtgaggtttt gaactgggga
    25681 tggatcgctt tgtctcgaca ttcacgaaca agatcgacgc gaaaggacgt gtttccatcc
    25741 cggcatcctt ccgcgcagtg ctcgagcgcg acggttatgc tgggggaatc tattgctacc
    25801 cctcgctcga ttctccggcg ctcgatgcgg gcggcgaaag acttgcgaag aagatcgatg
    25861 atcttctcgc ccatctcccg gactattcgg acgagcggga cgagctgtct gtcgcgctct
    25921 acggcgacgt gcagatcctc agcatcgacc aggacggacg catcgtcctt cctgagtcgc
    25981 ttcgcgccca cgcgggaatc accaatcagg tcacttttgt cggcctgggc gacaagtttc
    26041 agatgtggga gccggagcgc ttcgccgagc gccggcaaag ggcccgcgac aaggtgcatg
    26101 agcaccggaa gcttttcggc gcggggagcc gtcgcgataa tgacgaggga caaggcggcg
    26161 gcacaggagc acgggagtga tggcgggtcg cggatctcag gggggtctga gcaccgcgcg
    26221 cgaagagggc aaggatcgca gccgccacac tcccgtgctg ctcattccgg tcctgaagca
    26281 cctcgagcca aaggacggcg cgacgtatgt tgatgcgacg ttcggggccg gcggctacac
    26341 gcgcgcaatc ctcgacgcgg cgagctgccg ggtactcgcg atcgaccgtg atcctggcgc
    26401 agtcgccggc ggggccgatc tggtcgccgc ctatcacggc cggctcgaag tccgccaggg
    26461 gcggttcagc gagctcgaag agatcgcaaa ggatgccggc ctgaccgggg tcgacggcat
    26521 cgtgttcgac ctcggcgtct cctccatgca gctcgacgag gccgaccgtg gcttctcgtt
    26581 ccaggccgac ggcccgctcg acatgcgcat gtcgcgcgag gggccgactg ccgccgatct
    26641 cgtcaacacc ttggacgaga ccgagctggc agacattctc ttccatttcg gcgaggagcg
    26701 gaaagcgcgc gccatcgccc gcgccatcgt gagagcacgc aagcaggagc cgctgacgcg
    26761 cacgctgcag ctcgcccgtg tcgtgtcgca agtgctcggg ggccgcaaga tcgatggccg
    26821 tcatccggcc acccgcacgt tccaggccct gcgcatcgcc gtgaacgatg agttgggaga
    26881 ggtcgttgca gggctcgcgg ccgccgagcg gctcctgaag ccgggcggcc ggctcgtcgt
    26941 ggtaaccttc cattcactgg aagaccggat cgttaagcgg tttttagccg aaagggcggg
    27001 gaaaacggcc ggtacgtccc gccacctgcc gcaggccgcc cagcatcggg ccgaaagttt
    27061 ccgaattgtt aaccagcggc ctgttacccc tgacgaagag gagatcgccg ccaatccccg
    27121 tgcacggtcc gccaagctga gggccggcga gcgcaccgac gcgccgagct ggccgctcga
    27181 tccagcagcc ctgggggtgc cgcagatcac ccgatgagcg cgacaaccgc gaaaggcagc
    27241 ccatgcttcg catttcgtct gctatcgccg tcggtttggc tctgatctcg gccttcgtgc
    27301 tctacgcggt gagctacgag acgcgccatc tcgatacaga ggttgccgcg aaggagcgcc
    27361 gcatcgagcg gctacggagt gacatcgcgg tgctcaaggc cgagcgcgcc ttcctctctc
    27421 gcccggaaag aatcgagccg ctggcccgcg ccatgggctt cgtccccgcg gaagggtcgc
    27481 aatacgtcga gctcgattct ctgaagcagt ctgacgctcc gccggggcag cgcccctcca
    27541 gccggtgagg atcagttccc gtgtccaccg cacttggcca gcccgtcggc ccgccgcctt
    27601 cctcggcagg catcaactac gccgcacggg cgttcgccgt cgcgctggcg atcgttgtcg
    27661 ggttttctgc catcagcctc cagctcgtcc ggctggcgtt gagagggcag ctcgacgaac
    27721 cgcgggtcgc catgacccag ccgctgactg aatcctactg gcggcctgac atcgtcgacc
    27781 gtaacggccg cctgcttgcc accgacatcg aagcgcctac gctctatgca gacccggccc
    27841 tcatcatcga cgtggacgag gtcgtcgagc atctggttca ggtcttcccc gagttcgatg
    27901 cagttgagct tcggcgctcc ctgtccgacc gcaacagacg ctatgtgcgc ctgaagcgcg
    27961 gcatctcgcc cgccaccgcg cagcgcatcc acaacttcgg tttgccgggg atcgcgtttc
    28021 gcaacgagcc gaagcgggtc tatcccaatg gcgccctcgc cggacacgtc cttggctacg
    28081 tgaacatcga caacaggggc acatcgggtc tggagcgctt catcgacgag gccgtcggca
    28141 tcgaggcggt acactccggc gcgccgggat cgctgaagcc tgttcgcatc agcctcgatc
    28201 tggccgttca gtacgggctc gaggatgagc tgcgcgctgc cacgcggcag tatcgcgcca
    28261 agggcgcggc cggcgtcatt ctcgacgtca atacgggcga ggtgctcgcc gccgcctccc
    28321 tgccgcaggt cgatccgtcg gaccggtccc acatcctcga tgccagccgg ttggataaga
    28381 tgacggacgg cgtctacgag ctgggatcca ttttcaagat gcttaccgtg gccatggcgc
    28441 tcgaggcggg gacggcggac ctcgacaagg tctacgacgt gcgcgtgcca ttgcgggtcg
    28501 gacggtacac catccgcgat tttcatccgc tcggacggcc gctgtcggtc cgcgagatct
    28561 tcatccactc ctcgaacgtc ggcgcgggga tgatggcact tgagctcggc tcggagcgtc
    28621 agcgcgccta cctggccaag ttcgggctga tcgagcccat ccgcaccgag gcaggtcagg
    28681 tcgcgccgcc gaagctgccg cagaattggg gtaaggcgga aacgatcacg atctcgtacg
    28741 gccacggaat ggccgtcgcg cccctgcagt tcgctgccac ggcggcagcc ctgatcaacg
    28801 gcggctggca ggtgaggccc accttccgca gtgatccgat caccaccgac agggcccagg
    28861 tcatcagccc cgaaacgagc gccgccatcc gtgagctgat gcgccggaac gtgtcctcgc
    28921 ggcacgggac cgggcggcga gcggatgttc cgggctacga ggttggcggc aagacaggca
    28981 ccgcggagtt gcccgggcgg ggcggatatc gcgagacggc agtcatagcc tccttcgtcg
    29041 ccgcctttcc gatgagcgcc ccgaagtatc tcgtcctggt
//
LOCUS       DASVDU010000197        11026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_243875, whole genome shotgun sequence.
ACCESSION   DASVDU010000197 DASVDU010000000
VERSION     DASVDU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11026
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243875"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /gene="guaA"
                     /locus_tag="VF226_12660"
     CDS             complement(<1..1319)
                     /gene="guaA"
                     /locus_tag="VF226_12660"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7074885.1"
                     /translation="MIDTVLILDFGSQVTQLIARRVREAGVYCEIMPFDKAKEGLERL
                     RPKAIILSGGPSSVYDIGTPAPPPEVYQAGIPVLGICYGEQTMVAQLGGEVTPATHRE
                     FGRAEVEIVADCALFEGVWKKGMRDQVWMSHGDRVEKLPPGFEVIAVSANAPFAAIAD
                     EKRRFYGVQFHPEVMHTPRGAELLNNFVHKIAGCSGDWTMRAFREAAIERIRDQVGKG
                     RVICGLSGGVDSAVAAVLIHEAVDDQLTCIFVDHGLLRQGEADEVVSLFREHYHIPLV
                     HVRAEDTFLSALEGVSDPEAKRKTIGRLFIDVFEEEARKLGGAEFLAQGTLYPDVIES
                     VSFTGGPSVTIKSHHNVGGLPERMNLKLVEPLRELFKDEVRALGRELGLPESFISRHP
                     FPGPGLAIRVPGEVTREKLDILRRADAIYLEEIRKAGLYDTIWQAFAV"
     gene            complement(1404..1568)
                     /locus_tag="VF226_12665"
     CDS             complement(1404..1568)
                     /locus_tag="VF226_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074886.1"
                     /translation="MAMMVVVVMIMMIMTAATRIAMGVVMGMLALPTRAGLGVGAHLL
                     GPRCWLLKKI"
     gene            1605..1673
                     /locus_tag="VF226_12670"
                     /pseudo
     CDS             1605..1673
                     /locus_tag="VF226_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            2159..2896
                     /locus_tag="VF226_12675"
     CDS             2159..2896
                     /locus_tag="VF226_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074887.1"
                     /translation="MKWGTRYGADWVNRLAAMVRRNTSWSVRFVCFTDDPNGIRPDIE
                     CHPMPEVEFDRRIGEHWPKLGLMRKGIADLEGMTLYLDLDVVVIGNLDPFFTFPARFC
                     MVREWKDPHLGYGNSSVVRFFAGLESAVLDRFYATPPDIIARVYRRKEQNFLTRAVDD
                     VTFWPDEWCPAFSRECLPRNRILRYFSKPVPPTTGRILVFYGSITPASAMLGEHDPAK
                     RSRTRSSFPLGRRRFMPADWIGDYWRE"
     gene            2940..3761
                     /locus_tag="VF226_12680"
     CDS             2940..3761
                     /locus_tag="VF226_12680"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEX7074888.1"
                     /translation="MEELDPFQEGSADLRNAFLDLFRAVHERKRGVVVASSQRCGTHL
                     IGAYLRATGVGVPGEHFLSYLNARVGKTAEFEQLKKAVEQVIVGGSGGASDAFSVVLM
                     RYAQKIGDDLEALGLARSLLSRSMKNLTWIWLRRDWVDVAVSHYFAEKTGVWESRGGE
                     HIAPPYDAAGIHRWWWHVQVTDAFWERYFAENAITPIELDYAQLVKDPLVLTDAVSRA
                     GGNPALLSAAVPPPRMPHAQCKQDYAARFRDYLAERRSAELFKLHSAGRRSNAVA"
     gene            3820..4794
                     /locus_tag="VF226_12685"
     CDS             3820..4794
                     /locus_tag="VF226_12685"
                     /inference="COORDINATES: protein motif:HMM:NF016377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7074889.1"
                     /translation="MAFFNALGAIRAPSKLLKSYIGLRQYQNALVRIAGGIPRSDDAP
                     IPKLIHFVFGMRAPEELPYYAYLAIKSAQAQHEGWTIVFHYHHEPTGPYWAKIKRDVV
                     CIKVPKFAFYRFARFHHYAHKADVVRMLALYNAGGLYLDLDTICCRSFEPLRHNEFVM
                     GVQATIPGATGGLCNAIMASAPRARFLKIWLRRYASFRSRGRDALWDFHSVRLPVRLA
                     ARHPDLITVLPFNAFFYPLWPDLERLLLAENSGKYGHHLEQSFAFHLWNNMTAHALSQ
                     ITEDYVRTSQSNYARIARSALELVDESAPRLDRRGSSQRRQVPAAHAA"
     gene            4721..5632
                     /locus_tag="VF226_12690"
     CDS             4721..5632
                     /locus_tag="VF226_12690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7074890.1"
                     /translation="MKAPLVSIVAAVHNGAKYLPLMLHSILEQRYRPFELILVDDGST
                     DDIDGAIKPYQDSSGDIKVIRLARNMGLAAALNIGMKAASGEFIARMDADDYMYEWRL
                     RDQVLFLLEHPECDLVGSGADVFGATTDTWRSPFSQSDIVNTFLVGNPFLHPTIMFRR
                     RILLEELFIYNEHLSTEEDYELWSRLLPRLNCANLDKSLIRYRIHSSNNQRHPAKLRI
                     KELALRQFLAQIGCDEPQLVSALAEYQCSGFVRHEHYPVLRRYAHDAEARNWPRLGFL
                     HKAVLKAKSYRHFYELTSRKGLQFGLR"
     gene            5652..6431
                     /locus_tag="VF226_12695"
     CDS             5652..6431
                     /locus_tag="VF226_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074891.1"
                     /translation="MVALDSSEVRVIRPDASHVMRLLSALTPWKPVGFRKVRIGGEAD
                     GGYVMLDDFPSNPICYSFGIGGDVSWDLDMASRNATVYQYDHSIGKPPVQHPSFRFFR
                     LGIGDRNERGSLTTIVGALAENGHQGERGLILKMDIEDSEWSVLDATPESCLAQFSQI
                     VVEYHGLARAENRMWAARAESILRKVLRTHLPVHVHGNNWARYALVENIPVPDVLEVT
                     YAARACYSFAESDELYPTALDRPCKAGVPDLFLGSFKFAEV"
     gene            complement(6447..7487)
                     /locus_tag="VF226_12700"
     CDS             complement(6447..7487)
                     /locus_tag="VF226_12700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7074892.1"
                     /translation="MEQPKVSVLLPVHNAESFLAEAVESILGQSFKDFELICADDGST
                     DRSHEILSGYAARDDRVRILRREHIGLIAILNEGLEAARAPLIARMDADDRSTPERLE
                     QQYRRFELDARLWVLGTAEERIDPAGRPRGRPPVITGCEAVATALREKCVVRHPSVMM
                     RREPILALGGYRPAYKHAEDYDLWLRVAERGKIDNLGFIGLQYRMHSGSVSERHTARQ
                     RISAELARATHRLRISGKHDPTAALTVEPDLWNDPLLDQLIPAHAAFFRFIDLAFRAS
                     SDHFHDELAWRLLRNQDKTVVRANRRLWQEALTHMARVRSGWDGLRFASIIAALRTNP
                     GRFVRSSVGFFR"
     gene            7627..8730
                     /locus_tag="VF226_12705"
     CDS             7627..8730
                     /locus_tag="VF226_12705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7074893.1"
                     /translation="MHILVVLPRRSPTDRDIPNSVDLCVHDLVSASRHRSATCIVAER
                     VHKIFDAFETADIPSFAAAHSRLIARHVAHLAQARKPDLVVVHQHFQIAARLAPMLPG
                     RVVFHAHGFYKSYPKGLIAAVRRGMRLKEIRRLAGLVHVSEACRAHFDSSWPEVRLPQ
                     AVVHNGLDFRAWHPEALKRNEILCVGRCVPEKGILEAAQAVARVLPERPGWSARFVLS
                     ELDRNSGYSKRVEEVISQEPLRNRVETERNVSWLGVKERYERAAIALVPSRWREPFGR
                     TALEAHAGGAALVSSGSGGLSEVSGPFALFADPADVDGFAEAIRRLIDNEPLRRELSQ
                     GGAQYVRRRFSIESVAAVNDAFLERIRAGQNSC"
     gene            complement(8748..10541)
                     /locus_tag="VF226_12710"
     CDS             complement(8748..10541)
                     /locus_tag="VF226_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7074894.1"
                     /translation="MIVKDANTLRVLRRLVGEHGREYAPRYAIAFMFMGVVAGCTSLS
                     AWMMKDVVNKIFVDQDQAALIWIPLAISGLFVIKGIAAYLQEISLSRIGNRIVAQIQK
                     RMYDHMLKMGVGFYQQYPSSDLITRITQNAQAARGMLNLIAVGMGRDLLTLVGLIAVM
                     ISQDPILSAICLIGGPFAAVVLKRLSQRVQKAADRQYTGMSTIVGTMRESAQGIRIVK
                     SFQLERMLQRRMNDGIDGVEWLANKIVAVSALVNPLIETLGGLAVAMAVVYAGWRNVS
                     FGDTPGQFFAFITALLMAADPARRLSKLQLQLATAAIGVKMMYDLLDQPATEVEVDQR
                     PDLIASEGEIRFEGVTFSYDPDTPVLSELDLIAPAGKTTALVGLSGAGKTTVFNLLQR
                     FWEPARGVIRIDGQPISEYSISSLRRQIALVSQDVFLFEGTIRENIVAGRKDASEEEI
                     VAAAKAAQADEFIRSLPNGYDTQVGELGSQISGGQRQRISLARAFLKNAPIILLDEPT
                     SALDSETEQAIQSALQKLTRGRTTIVIAHRLATVANADLIHVMEKGRVVESGRHRELI
                     ERGGLYARLYQIQFANAIPSPKERDIAAASA"
     gene            complement(10813..10980)
                     /locus_tag="VF226_12715"
     CDS             complement(10813..10980)
                     /locus_tag="VF226_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074895.1"
                     /translation="MTESGVFEVLLGAVGAIGSIASRHEDPFSAGMVFLASMLLVGLG
                     VAQLRKFKNRL"
BASE COUNT         2068 a         3416 c         3337 g         2205 t
ORIGIN      
        1 accgcgaatg cctgccagat cgtgtcgtaa agcccggcct tgcggatctc ctccaggtag
       61 atcgcgtcgg cacgccgcaa aatatcgagc ttctcgcgtg tgacctctcc cggcacacga
      121 atggcgaggc ccggacctgg aaacgggtgc cggctgatga aactttcggg caaccccagc
      181 tcgcgcccga gggcccgcac ctcatccttg aacagctcgc gcaggggctc gaccagcttg
      241 aggttcatgc gttcgggcag gccaccgacg ttatggtgcg acttgatcgt caccgacggg
      301 ccgcccgtga aggaaacgct ctcgatcacg tccgggtaaa gcgtaccctg ggccaggaac
      361 tcggccccgc cgagcttgcg cgcctcctcc tcgaacacat ctatgaagag ccgcccgatc
      421 gtcttgcgct tggcttcggg gtcgctcacg ccttcgagcg cgctcaggaa cgtatcctcg
      481 gcccgcacat gcacgagcgg gatgtggtag tgctcccgga acagcgatac gacctcgtcg
      541 gcctctccct gccgcagcag gccgtggtcg acaaagatgc aggtgagctg gtcatcgacg
      601 gcctcgtgga tgagcacggc agcgacggct gaatccacgc cgccggacaa tccacagatg
      661 actcgtccct tgccgacctg atcgcggata cgctcaatgg ccgcctcgcg gaacgcccgc
      721 atggtccagt cgccgctgca accggcgatc ttgtgcacga aattgttgag cagctcggcg
      781 ccgcgcgggg tgtgcattac ctccgggtgg aactggaccc cgtagaaccg gcgcttctca
      841 tccgcaatgg ccgcgaatgg ggcgttggcc gagaccgcga tgacctcgaa tcccggcgga
      901 agcttctcga cgcggtcgcc gtggctcatc cacacctggt cgcgcatgcc cttcttccag
      961 acgccttcga acagcgcgca gtcggcgact atctccacct cggcgcgtcc gaactcgcga
     1021 tgggtggcag gcgtcacttc gccgccgagc tgcgcgacca tggtctgctc gccgtagcag
     1081 atccccagaa ccggaatgcc ggcctgataa acctctggag gtggcgcggg cgtgccgatg
     1141 tcatagacgc tggaagggcc gccggagaga atgatggcct ttggtctcag gcgctcgagc
     1201 ccttccttgg ccttgtcgaa gggcatgatc tcgcagtaaa cccccgcctc gcgcacgcgc
     1261 cgggcgatga gctgcgtcac ctgcgagccg aaatcgagaa tgaggacggt atctatcatg
     1321 acgagccgtt acgataatcc ccgcgcgact gcaacgggct ctggggtgcg accaacaggc
     1381 gtctgcgggg catcgcaccc gcgctatatc tttttcagca gccaacatcg aggtccgagc
     1441 agatgagcgc cgacaccaag accagcgcgg gtgggcagag ccagcattcc catgaccacg
     1501 cccatggcga tgcgtgtggc tgcggtcatg atcatcatga tcatgaccac caccaccatc
     1561 atggccacgc gcacatcgcg ccttatgtcc gctccgggcc gaaggtcggc cgcaacgacc
     1621 cctgcccctg cgggtcgggg aaaaagtaca agaagtgttg cctggcggca taggcttggc
     1681 gactgcgatc cgtatatcta acgcctcact tggcccctcg ggccgacctc tctgtgggag
     1741 aggtgaagta gtaggcccag ccgtttccat atagatcgga aaagctagag gaagggctgc
     1801 gggtgcgtgg gcacggtgtt ggctggacac gaggctttga tcctcggcgc ccgattctcc
     1861 gtcgctgctc gcttgccttg tgaatcagat cctcacgcca gcttgccgca aggctcgttc
     1921 gtgatgttgc cccggtcggt actcgttatg tactcctggg tgacacgcca tggttgcgca
     1981 agacttcggg tggcacagcc cggctgagag cggggcaact gtccccggcg gagccgtacc
     2041 agccctgacc tcatggggca agaggcttcc gaatgaagac ccggctggcc ttgtcgaaag
     2101 cgattggcgc cttcaattcg agcgggctca gcacagccgg cgcgccaatg tggtctgcat
     2161 gaagtggggc acgcgctacg gggcggactg ggtgaaccgg ctggccgcca tggttcggcg
     2221 caacacgagt tggagcgtcc gcttcgtttg cttcaccgac gatccgaacg gcattcgccc
     2281 ggatatcgaa tgccatccca tgccggaggt cgaattcgat cggcgaattg gcgagcactg
     2341 gccgaaactg ggcctcatgc gaaagggaat tgccgacctc gagggcatga cgctctattt
     2401 ggatctcgac gtcgtggtca tcggcaatct cgatccgttt tttacgtttc cagcacgctt
     2461 ctgcatggtg cgcgaatgga aggacccgca cctcggttac ggcaacagct cggtcgtacg
     2521 tttttttgcg gggctcgaga gcgccgttct cgatcgcttt tacgcaactc caccggacat
     2581 catcgcccgc gtgtacaggc gcaaggagca gaatttcctc actcgggcgg tcgacgacgt
     2641 gacattctgg cctgacgagt ggtgcccggc cttctcgcgt gaatgcctgc cgcgaaaccg
     2701 aatccttcgc tatttctcca agcctgttcc gccgacaacg ggccgcatcc tcgttttcta
     2761 cggctcgatc acgccggcct ctgcaatgct tggcgagcac gatccggcga aacgctcgcg
     2821 cacccgctcc tccttccccc tcggccggcg ccgcttcatg cctgccgatt ggattggaga
     2881 ttattggcgg gagtagccac gaagactcca gcgacgctcg cctacggggg acgattacaa
     2941 tggaagagct cgatccattt caggagggga gtgcagacct cagaaatgcc tttctcgacc
     3001 tgttcagggc agtgcacgag agaaagcgtg gggtcgtcgt tgcttcctct caacgctgcg
     3061 gcacgcatct catcggtgca tatctgcggg caacgggcgt cggcgttccg ggtgaacact
     3121 ttctgagcta cctgaacgct cgtgtcggca aaaccgctga atttgagcag ctcaagaagg
     3181 ctgtcgagca ggtcatcgtt ggcggttcgg gaggggcatc cgacgccttc tccgtcgtgc
     3241 tcatgcggta tgcgcaaaag atcggcgatg acctagaagc gctcggcttg gcccggtcgc
     3301 tcctttcccg atcgatgaag aacctgacct ggatctggtt acgccgggac tgggtcgacg
     3361 ttgcagtgtc gcactacttt gcagaaaaaa cgggggtttg ggagagccgg ggcggcgagc
     3421 acatcgcgcc gccctatgac gcagcgggga ttcatcgctg gtggtggcac gttcaagtaa
     3481 cggacgcatt ttgggagcgc tattttgcgg agaatgcaat tactcccatc gagctggatt
     3541 acgcgcaact cgtgaaggat cccctcgtcc tgacggacgc cgtcagccgg gccggcggaa
     3601 atccggcttt gttgagcgct gccgttccgc caccgcggat gccccacgcg caatgcaagc
     3661 aggactatgc cgcacggttc cgcgactacc tcgccgagcg gcggtcggcc gagcttttca
     3721 agctgcattc ggcggggagg aggtcaaatg cagtggctta gggatcgcat ccggcgtgct
     3781 ctcgagcgcc ggatgacgcg agacaaatgg cattatgcgg tggctttctt caacgccctc
     3841 ggtgcgatca gggctccttc caagctcctc aaatcgtata tcggtctgcg ccagtatcag
     3901 aacgcgcttg tgcggatcgc cggcggcatt ccacgttccg atgatgcgcc catcccgaag
     3961 ctcattcatt tcgtgttcgg tatgcgcgcg ccagaggagc tgccgtatta cgcctacctc
     4021 gctatcaagt ctgctcaagc ccaacatgag ggttggacaa ttgtcttcca ttaccaccat
     4081 gagccgacag ggccgtattg ggcgaaaatc aagcgggacg tcgtttgcat caaagttccg
     4141 aaattcgcgt tttaccgatt cgcccgcttc catcattacg cgcataaggc tgatgtcgtc
     4201 cgcatgctgg cgctctacaa cgcgggtggc ctctatcttg atctggatac gatctgctgc
     4261 agatcattcg agccattgcg gcacaacgaa ttcgtcatgg gggtgcaggc gacgattccc
     4321 ggcgccaccg gcggcctgtg caacgcgatc atggcttctg ccccccgcgc caggtttctg
     4381 aagatctggc tccggcgcta cgcatcgttc cggtccagag gccgggatgc gctttgggat
     4441 tttcattcgg ttcggctgcc ggtccgtttg gcggcgcggc acccggatct catcacggtc
     4501 cttccattca atgcattttt ctatccgcta tggccagacc tggaacgtct cctgcttgca
     4561 gaaaattccg gtaaatacgg gcatcacctc gaacagtcgt ttgcattcca cttatggaac
     4621 aacatgacgg cgcatgccct ttcgcaaatc actgaggatt acgtgcggac ctcgcaaagc
     4681 aactacgccc gtattgcccg ttctgcattg gagttggtcg atgaaagcgc ccctcgtctc
     4741 gatcgtcgcg gcagttcaca acggcgccaa gtacctgccg ctcatgctgc atagcatcct
     4801 ggagcagcgg tacaggccct tcgagctcat ccttgtcgac gacgggtcca cggacgacat
     4861 tgacggcgcg atcaagccgt accaagactc gagcggcgat atcaaagtca tccgccttgc
     4921 cagaaacatg ggccttgcag cggccttgaa tatcggaatg aaggccgcga gcggcgagtt
     4981 catcgcccgg atggacgcag acgactacat gtacgaatgg cgtctgcggg atcaggtcct
     5041 cttccttctc gagcacccgg aatgtgacct cgtgggttcg ggcgcggatg tcttcggcgc
     5101 aacaacggac acgtggcgtt cgcctttctc ccaatccgat attgtgaaca catttctcgt
     5161 cggcaatccc tttctgcacc cgaccatcat gttccgacgt cgtattcttc tggaggagct
     5221 cttcatctac aacgagcact tgtcgaccga agaggattat gagctctggt cgcgcttgct
     5281 cccgcggctg aactgcgcca acctggacaa gtcgctcatc cgataccgaa ttcattccag
     5341 caacaatcaa aggcatccag caaaactgcg catcaaggag ctggcgcttc gccaatttct
     5401 ggcgcagatc ggctgcgatg agccgcagct cgtgagtgct ctcgccgagt atcagtgcag
     5461 cggcttcgtt cgccatgagc actatccggt tcttcgtcgg tacgcacatg atgccgaggc
     5521 caggaattgg cccaggctcg gcttcctgca taaggccgtt ttgaaggcaa aatcctaccg
     5581 gcacttctat gagctgacgt ccagaaaggg gctgcagttc ggattgaggt gatcatgatg
     5641 gagagacgcg aatggtagcc ctcgatagca gtgaggtcag ggtgatcaga cctgatgcat
     5701 cccatgtgat gcggttgctc tctgccctca cgccctggaa accggtcggc ttcagaaagg
     5761 tccggatcgg tggagaagcg gacggcgggt acgtcatgct cgacgacttc ccgagcaatc
     5821 ccatatgcta ttcattcggc atcggaggtg acgtgagctg ggacctcgac atggcgtcgc
     5881 ggaacgcgac ggtctatcaa tacgatcact ccataggcaa gccacccgtt caacacccga
     5941 gctttcgctt tttccggctg ggaattggcg accgcaatga acgtggtagc ctgacgacca
     6001 tcgttggcgc gcttgccgaa aacggacacc aaggcgaacg cggcctcatc ctgaaaatgg
     6061 atatcgagga cagcgaatgg tcggtgcttg atgccacgcc cgagtcgtgt ctggcgcaat
     6121 tcagccagat cgtcgtcgag tatcacgggc tggcgcgcgc ggagaataga atgtgggccg
     6181 cgcgagcgga atcgatcctg cgtaaggtgc ttcggacgca tttgccggta catgtgcacg
     6241 ggaataattg ggctcgctat gccctggtgg agaacatacc cgttccggat gttctggaag
     6301 tcacctacgc cgcgcgggcc tgctactcct ttgccgagtc cgacgagctt tatccaacgg
     6361 ctctcgacag accgtgcaag gcgggagttc ccgacctctt cctgggcagc ttcaagtttg
     6421 ctgaggtttg atttgctcag cttcaattac ctgaaaaagc caaccgacga gcggacaaat
     6481 ctcccagggt tcgtacgcaa agcagctatt atgctggcga atctcaatcc gtcccagccg
     6541 cttcgcacgc gtgccatatg cgtcagcgcc tcctgccata gccgccggtt ggcgcgtacg
     6601 acagttttgt cctgatttcg aagcaggcgc catgccagtt cgtcgtggaa atgatctgat
     6661 gatgcccgga aggccagatc gatgaaccgg aagaaagccg cgtgcgccgg gatgagctga
     6721 tcgagcagcg gatcgttcca taggtccggc tcgactgtca gagcggctgt ggggtcgtgc
     6781 ttgcccgaaa tccgcaggcg gtgcgtcgcg cgcgcgagct ccgcggaaat cctttgccgt
     6841 gccgtatgcc gttccgacac gctgccagaa tgcattctgt actgcaggcc aatgaacccc
     6901 agattgtcga tctttccgcg ctcggcgacc cgcaaccaaa ggtcgtaatc ctccgcatgc
     6961 ttataggctg gccggtaacc gccgagcgcc aatatcggct cgcgacgcat catgacagag
     7021 gggtggcgca cgacgcactt ctcgcgcaat gccgtggcta cggcctcgca accggtaatg
     7081 accggagggc gccctctcgg gcggccggcc gggtcaatcc gttcctcggc ggttccgagc
     7141 acccaaagcc gagcatcgag ctcgaaccgg cgatactgct gctccagccg ttccggtgtc
     7201 gatctgtcgt ccgcatccat gcgcgcgatc agtggtgcgc gtgccgcctc gagcccctcg
     7261 ttcaggatcg caatcagccc aatgtgctct cgacgcagga tccgaacgcg atcgtcccgc
     7321 gccgcatagc cgctcaggat ctcatgcgac cgatcggtcg atccgtcatc ggcgcagatg
     7381 agctcgaagt ccttgaacga ctgtcccagg atgctttcca ccgcttctgc cagaaaactc
     7441 tcggcattgt gcacaggcag aagcacgctg actttgggtt gttccattct tgtaccagtt
     7501 ttgttctaat gacgctgaca ttgccgcgag acgcgggttt gcgtcaacgg caaaggtcag
     7561 aggttgtccg gaacgggtga gctgcgctcc agcgccgttc gcttgcgatt tggttggagt
     7621 tggacagtgc acatccttgt cgtgttgccc cggcgatcgc cgacggaccg ggacattccc
     7681 aactccgtcg acctgtgcgt tcacgacctt gtttccgcga gccgccatcg gagcgcgacc
     7741 tgcatcgtgg ccgagcgcgt acataagatc ttcgacgcgt ttgaaacggc tgacatcccg
     7801 tctttcgcag cggcgcatag ccggctcatc gccaggcatg tggcccacct cgcccaggca
     7861 agaaagccgg acctcgtggt ggttcatcag cattttcaga ttgcggcacg gctcgccccc
     7921 atgctgcccg gccgcgtcgt tttccatgcg catggcttct acaagagtta tcccaagggc
     7981 ctcattgccg ctgtccggcg cggtatgcgt ctcaaagaga tacgccgcct ggctggtctg
     8041 gtgcatgtga gcgaggcgtg ccgtgcgcac ttcgacagct cctggccgga ggtccggctg
     8101 ccgcaggctg tggtgcataa cggactggac ttcagggcgt ggcaccccga ggcgcttaag
     8161 cgaaacgaaa tcctgtgtgt cggacgctgc gtgccggaga agggcattct cgaggcggct
     8221 caggcggtgg cccgcgtgct gccggaacgg ccaggctgga gcgcacgatt tgttctttcg
     8281 gagctcgacc gaaattccgg ctatagcaag agggtggaag aggtcatctc ccaggaaccg
     8341 cttcgcaatc gcgtcgaaac ggaacggaac gtttcctggc tgggcgtgaa ggagcgctac
     8401 gagcgggctg ccattgctct cgtcccctct cggtggcggg agccgttcgg ccggacggcg
     8461 ctcgaagccc acgccggcgg cgccgccctc gtttcgtctg gttcaggcgg actgagcgag
     8521 gtcagcggcc catttgcact atttgccgat ccggcggacg tcgacggatt tgccgaggcg
     8581 atccgacgat tgatcgacaa tgagcctctg agaagagagc tgtcgcaggg tggcgcgcag
     8641 tatgtgcgcc gacgcttctc gatcgagagc gtggcagcgg tgaatgacgc ttttctcgag
     8701 aggatccggg cgggacagaa ctcctgctga gaggctgggg cgcaagatca ggcgctggca
     8761 gcagcgatgt ctctttcctt agggctgggg atcgcgttgg cgaactggat ctggtacagg
     8821 cgcgcataga gcccgccccg ttcaatcagc tcgcggtgac ggccgctctc caccacccgg
     8881 cccttctcca tgacatggat gaggtccgca ttcgccaccg tggcgaggcg atgcgcgatg
     8941 acgatcgtcg tgcgcccgcg ggtcagcttc tgcagggcag actggatggc ctgctcggtc
     9001 tcgctgtcga gagcggacgt cggctcgtcg agcaggatga ttggcgcgtt tttcaggaaa
     9061 gcccgggcga gtgaaatgcg ctggcgctgg ccgcccgaga tctgggagcc aagctcgccc
     9121 acctgcgtgt catagccgtt cggcagcgag cggatgaact catccgcctg cgcagccttg
     9181 gcagcagcca caatctcctc ctcggaggcg tctttccgtc ccgcaacgat gttttcacgt
     9241 atggtgcctt cgaacaggaa cacgtcctgg ctgacgaggg cgatctgccg ccgcagggac
     9301 gagatcgagt actctgagat cggctggcca tcgatgcgga tgaccccacg cgccggctcc
     9361 cagaaccgtt gcagcaggtt gaaaaccgtt gtcttgcccg ctcccgagag gccgacaagc
     9421 gccgtcgtct tgcctgctgg tgcgatcagg tcgagctcgg acagaacagg ggtgtccggg
     9481 tcgtaggaga acgttacgcc ttcgaaccga atctcgcctt cgctggcgat caggtccgga
     9541 cgctggtcca cttcgacttc cgtggccggc tgatccagca ggtcatacat catcttcacg
     9601 ccgatcgccg cggtggccag ctgcaattgc agcttggaaa gccgccgcgc gggatcggcg
     9661 gccatgagca gggcagttat gaaggcaaag aattgccccg gtgtgtcgcc gaaggatacg
     9721 tttcgccatc ctgcatagac caccgccatc gcgacggcca agccaccgag ggtctcgatc
     9781 aacggattga ccagcgcgct cacggcgacg atcttgttcg ccagccactc gacgccgtcg
     9841 atgccatcgt tcatacgccg ctgcagcatc cgttcgagct ggaacgactt gacgatgcgg
     9901 atgccctggg cgctctcgcg catggtcccg acgatcgtcg acatgccggt gtactgcctg
     9961 tccgccgctt tttgcacgcg ctgcgacagc ctcttaagaa cgactgctgc aaacggcccg
    10021 ccgatcaggc agatcgccga gaggatcggg tcctgcgaga tcatcacggc aatcagccca
    10081 accagcgtca gcagatcacg acccatcccg acggcaatca ggttgagcat gccccgcgcg
    10141 gcctgagcat tctgggtgat gcgtgtgatc aggtcactcg atggatactg ctgatagaag
    10201 ccgacgccca tcttgagcat gtgatcgtac atgcgcttct ggatttgggc gacgatgcgg
    10261 tttccgatgc ggctgagcga gatctcctgc aggtaggcag ctatcccttt gatgacgaaa
    10321 aggcccgaga tggccagcgg gatccagatc agcgccgcct ggtcctggtc gacgaagatc
    10381 ttgttcacca cgtctttcat catccaggcg ctgagggatg tacaccctgc cacgacgccc
    10441 atgaacatga aggcgatcgc gtaacgcggc gcatattcgc ggccgtgctc gcccaccagg
    10501 cgccgcagca cccggagagt gttcgcgtct ttgacgatca tgccggaaat cagccacctg
    10561 cccgacggca ggccgtctcg atgttgctca tggccccacc catcaactcg agcactcgaa
    10621 tcactttaac atcgcgcgta gaacttgacc aagttcgacg tggtctgcac gtcgctgcaa
    10681 cttagccgtg cagtcctgcc cgaggctggc ggcgagatga tggcaagaag cttgcctctg
    10741 gcagaccatc accgagctga gtaagtcaag gccgggccgc aggtctcggt ccgcatctgc
    10801 ggcgtacgga tgctaaagcc gattcttgaa tttcctgagc tgcgccacgc cgaggccgac
    10861 gagcagcatg ctcgccagga agaccatgcc agccgaaaag gggtcctcgt ggcggctggc
    10921 gatgctgccg attgccccta ctgcccccaa aagcacctcg aacacgcccg attcagtcac
    10981 accaccccct catcgctgct caagtcggct gcggtcccgg cccgct
//
LOCUS       DASVDU010000198        24712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_787221, whole genome shotgun sequence.
ACCESSION   DASVDU010000198 DASVDU010000000
VERSION     DASVDU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 24712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24712
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787221"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VF226_12720"
     CDS             <1..1509
                     /locus_tag="VF226_12720"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX7074896.1"
                     /translation="LNAADRSAGSEADVVPADGSAELIIDGGEFQIAGPQDQAQAGDA
                     GPAAPLIKALRALNFDTVVIRQGTVRLALPDGRIETLTNVLAEVRRDGRSSIGARGTA
                     RFRGHEFSFDLSAPATANASAVAGVPMMLRIKSPLMDVAFDGGIGASDRLHLHGRIEF
                     AVPNVRSAARSFGAPWPDGPGLRDVAIKGDFHWQSPALTFDKASFRMDGNEATGTLAL
                     SFAGERPALTGTLALQSLSLAPYISPDGATDNSFSSFLAWARSDRSELSAPMARYFDA
                     DVRLSAGQLVAGGTTFGRFAASLSLKRGRLLADIAEIDLTGGRGSGQITADLAGTLPQ
                     ISVRGKLEDIDAARASMALMGHSAVQGLSTVSMDLTAEGNSLSELLSGVRGKIALNVY
                     EGGRLGIDIRALLDAAQKGALEGWAVGRGQTSVEGLQAKLRVDKGILASELVEATAGN
                     SLLRAFGTISLQSRQMDLRLLLDGLGDSKPDVAGDVLVFRGPWTSPSITLQR"
     gene            complement(1581..2216)
                     /locus_tag="VF226_12725"
     CDS             complement(1581..2216)
                     /locus_tag="VF226_12725"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEX7074897.1"
                     /translation="MSIVSLVRGLACEAGAGVRARAGNKILPALLLGAAAMMCAPAES
                     RADSGNEWLKAAFAKSPSAVRFDQPSRLGAYMPDTDRRRPRKTKRVRTASLGPMPIPS
                     TEPPPSATGGGTIHWKASASCLNAELRAAIEHVAANYGKVRVNSTCRSRKHNRRVGGA
                     PRSYHLTGNAADIRVFGNVRSALAYLRSAVGGLKHYGGGLFHIDTGPRRRF"
     gene            complement(2639..3019)
                     /locus_tag="VF226_12730"
     CDS             complement(2639..3019)
                     /locus_tag="VF226_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074898.1"
                     /translation="MEKINQLAHVSVARACGFAMLAIATFMVGLSSQFALALRAGGFG
                     CLLLSLVLIGMSWRAASTPYQRTELWLMLDPSERPQAAVAQSIISTARREAFLDFALR
                     SAWLATTLLVMALFWGFLPSRAAF"
     gene            complement(3119..3469)
                     /locus_tag="VF226_12735"
     CDS             complement(3119..3469)
                     /locus_tag="VF226_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074899.1"
                     /translation="MTGADNREDLLKRDMLLVGAAAVSLLNGMHFSPYFDPVFILLRP
                     FIAGTLLSTPLVLFYITSIFISLMTLLIAGVPAALYERFRGLHESNATSLGIWLGATL
                     ILALPTLMSLAGRS"
     gene            complement(3473..3784)
                     /locus_tag="VF226_12740"
     CDS             complement(3473..3784)
                     /locus_tag="VF226_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074900.1"
                     /translation="MSVAESFFTYWYFHIPNLILAALIYTLIGRYLLTLFFRKNPDAV
                     MLRVFQSITDPVLKAIRVVTPAIVPNGLVMVFAIAWLMAARMFWFLTCVAAGMSADVG
                     V"
     gene            complement(3889..5895)
                     /locus_tag="VF226_12745"
     CDS             complement(3889..5895)
                     /locus_tag="VF226_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEX7074901.1"
                     /translation="MADQAPAARGDFISNLGKIYGLYTGGFFAFVIFLAILEQLGVPN
                     QWIGYGFVFMTIAVYAVIGIISRTTEVGEYYVAGRRVPAVYNGMATGADWMSGASFVG
                     MAGTLFLLGYDGLAFVLGWTGGYVLVAILIAPFLRKFGAFTVPDFFSERYGGNFSRFL
                     AVIVLICCSFTYVTAQVYATGIIASRFLGMDFTVAVYVGLVGILLCSMLGGMKAVTWT
                     QVAQYIVLIVAYLIPVIILSTQIYGIPVPQLTYGQALRDIGALEQNMLTSGLATAQSL
                     KPHIQPFTTYDPLNYFALILCLMVGTASLPHILMRYFTTPSVREARLSVAWSLFFIFL
                     LYFTAPAYAAFSKLEVYSTVIGRDLDSIRPWLFTYGQLGLVKICGADAIDVAAIKAAC
                     SQIAGHPGVIRLQDFSINTDVIVLSTPEIAGMPYVIAGLVAAGGLAASLSTADGLLLA
                     IANALSHDIYYKMLDRNAPTTRRLIVARILLVTVAVAAAYTASTKPADILSMVAWAFS
                     LAAAGIFPALVLGIWWKRANTAGCIVGMVLGFGICLYYLVGTRYFAVHFYETWSWLSN
                     ASPAAIAKFNELKAAWMNAADGAARDAALVALDKHAQTIANWWGVRNISAAIFGLPVG
                     FLAIWIVSLLTPEPSKEVQEMVDATRRPRGQTILRDKDAPAVAH"
     gene            complement(5906..6160)
                     /locus_tag="VF226_12750"
     CDS             complement(5906..6160)
                     /locus_tag="VF226_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX7074902.1"
                     /translation="MEQGEDRYWRRTSSLMWTMLALWAFFSFVIHFFVNPLNEIQILG
                     FPLGFYMAAQGSLIAFVVMLFFYAWAQDKIDREEGVAEDE"
     gene            6442..8547
                     /locus_tag="VF226_12755"
     CDS             6442..8547
                     /locus_tag="VF226_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEX7074903.1"
                     /translation="MPNSSFATPLVSLSAVVLDLETTGLDARSARAVQVGAVRIDEGR
                     IVEDAKLDRLVNPGIPIPPAATAIHGITDAMVADAPRFAELLPEIEAFIGDAIVIGHT
                     IGYDLSVMRREYELANRAWRQPRSLNLPTLAKLAAPTLAHHSIDHLCNWLNVPKTGRH
                     TALGDAETTARLFLALIPLLRERNIRTLAQAESASRQLAEAEARATGGLLPSPGVAIS
                     PQEPRPLARIDSFPYSHLVRDVMSAPPVFADASASVREGIQILLDKNVSSVFVRNNAG
                     ELGIVTERDLLRAINKDGDAGLDAPLEAIMTKPLQTVPEDAFVYRALGRLERMGFRHL
                     GVHDAHGEIIGALTRRNLLRHRSTSVIMLGDEVDSATSVAALGAAWAKLPAVARSLVA
                     DDVDPRTISAVISSEIRAITRRAAELAEAKLAAEGQGRAPCAFAVLVLGSAGRGESLL
                     AADQDNAIVYETGAEGGAEDLWFEALGKEMCQTLHEVGIPFCKGGVMAKNRAWRLSLS
                     DWKAVIDSWVHRQRPEDLLNVDIFFDSVPVHGDATLGEAVWNYAFERGHSAPDFLKLL
                     IEVARQRSPAFTLFGNIRIDEKGRIDLKRAGLMPIFTCARVLSIRHDVRARSTPERLE
                     GVAARGIGSPHEIEEIIEAHRTILGAMLAQQIDDAEHGMSLSSRVNPDRLGKARKRQL
                     SKALGKVDAVIDLVSEGRL"
     gene            8668..10236
                     /locus_tag="VF226_12760"
     CDS             8668..10236
                     /locus_tag="VF226_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7074904.1"
                     /translation="MQVLRTDVAIIGGGLGACAAALAAARLGCRVILTEETHWIGGQL
                     TNQAVPPDEHPWIEEFGATATYRALRNGIRQYYRDHLPLTTLARASEHLNPGNGWVSR
                     LCHDPRVAVAVLHQMIMPYQANGKMFVMHPQRQLAAWTHGDRVTGVVVRGLESGRDTL
                     IEASYFIDATPQGELLDLAGVEHVCGAESRAETGEPHAPEMADPRDQQAITVCFAMEY
                     IPGEDHTIDKPRQYELWRSYQPPGWPGPLLGWTTQKPETHEPITRYLFEAEDGKPWWN
                     FRRILDVSNFEEGFGAHEISVVNWPQNDYWFGPVCDVDEVEQVRNIEAARQLSLSLLY
                     WLQTEAPRPDGGVGYRGLRLRGDVVGGTVDGLAQAPYIRESRRIKAEFTVLEQHIAYP
                     LRPDGPEFFEDSVGIGCYRIDLHPSVSGKGYLDLGCWPFQIPLGSLIPVRVENLLPGG
                     KGLGVTHITNGAYRVHHVEWNVGEAAGLLAAFCLERKVTPRAVRNTKALLEDFQSLLR
                     NQGIPLTWPSLTPL"
     gene            10321..11415
                     /locus_tag="VF226_12765"
     CDS             10321..11415
                     /locus_tag="VF226_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX7074905.1"
                     /translation="MNLGDEAILDGILSQLRSSLSAEITVFSQNPKDTLSRHKVERAV
                     PCRDMTRKEVAREIERLDLFVLGGGGILYDRDVHRYLREVNIAHELKVPVFIYAVSAG
                     PLTLTATRNAVRDALNLAAVITVRDRQGYRLLEDVGVDREIHLTADPALLIDPEEIPR
                     TLLRAEGVEFERPLVGFSVREPGPAAPDIDPQHYYALLANTADFIVDRLDADVVFVPM
                     ERTDVQHSHGVVAQMHRSERAEVLRRKYTAHQTLGLMDTFEFAVGMRLHFLIFAALRG
                     TPFVALPYASKVMGLVEDLEMDIPPLGDIGTGQLIAMIDRSWDTRDEIRHKIKCCLPP
                     LQVRARKTNQLLVDLLRKQSGSAGLKSATA"
     gene            complement(11428..12351)
                     /locus_tag="VF226_12770"
     CDS             complement(11428..12351)
                     /locus_tag="VF226_12770"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7074906.1"
                     /translation="MKIAIIGVGAMGSVYAALLASGGHDVWALDTWQEHIDAIREKGL
                     RVEGASGDRTVRLNATTDPADIRDADLVVIATKDQGVVDAAHAALRIAKADAPILTIQ
                     NGLGSADKVAEIVGSNRIMMGVVGGFGASIKAPGHAHHNGMEFLRLGEMDGGQTPRLA
                     AVAEAWRAGGFNVLTFDDIHKMVWEKLICNVAYSGPCTLTGLRVGEAQANPQAWSICA
                     ACANEAYLVARAKGIAVDFTDPVAYVRAFGEKIPNARPSMLLDHMAGRRAEIDNINGA
                     IPREGAKVGVATPVNSVVVALLKAKEACFDV"
     gene            12573..13916
                     /locus_tag="VF226_12775"
     CDS             12573..13916
                     /locus_tag="VF226_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX7074907.1"
                     /translation="MRFALSIVSLLAGLVIFLGQPVKGQDAGGGPVLVFDVKGVIGFT
                     SAQHLERSVERAKAEKASIIIMRIDTPGGLVSSTRDMIQTILASPIPIVGYVAPSGAR
                     AASAGTYLMYASHLAAMAPATHLGAATPIQMGAPGITPSPSEKKDQPSEPDAAERKLI
                     NDAVAYLRGLAELRNRNADWAEKAVREGATLTSTKALEEKVIDFLAPDLREVIDKAHG
                     RTVTTSAGTVTLDTRGKRIVSVEPSWQMQVLEVIADPNVAFILMLLGIYGIIFELLNP
                     GALFPGVIGGICLVLALTALTVLPVNLGGIALLLLGIALLAAEAFAPGFGVLGIGGIV
                     AFILGGLFLFDPDQVDIQFEVSRPLVVGAAIASAIILAGVLGMAMQSRKREVQTGGEQ
                     LIGSTAEVVEWSGHAGRVRVLGEIWLAESDVELARGDRVKVTAREGLKLTVKQTT"
     gene            13938..14717
                     /locus_tag="VF226_12780"
     CDS             13938..14717
                     /locus_tag="VF226_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7074908.1"
                     /translation="MDALTTSLIPIVIILLILASAIKILREYERGVVFTLGRFWGVKG
                     PGLIIIIPGIQQFVRVDMRTRVLDVPPQDVISRDNVSVRVNAVIYFRVIDAERAVIQV
                     EEFESATSQLAQTTLRSVVGQHDLDEMLAARERINNRIQDILDEQTDAWGIKVSNVEL
                     KHVDLNETMVRAIAKQAEAERLRRAKVIDAEGELQASEKLRQAGDVLAQSPYALQLRY
                     LSTLINIAGERSSTIVFPVPMDLITKWSQGGQGAPPKSEAQ"
     gene            complement(14739..15695)
                     /locus_tag="VF226_12785"
     CDS             complement(14739..15695)
                     /locus_tag="VF226_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7074909.1"
                     /translation="MQEPARLQRLLLATDLSSRSDRAFHRAAELARTNKALLTVLHVI
                     PDELNPDYADALKTSAKAALEKQVSQEMAAGEFNVNIAVKGGLDEEAILKQVREGAAR
                     GDFSVEVRIEAGSIYDIIIQTADAANADLIICGVHHRLMIGDEWLGSTIDRVLRFGNR
                     PVLVVKSEPENSYKSIVVGVDFSEASAEALAFAYAAFPSAKFTLLNAVESSFSGFLSG
                     ADASQEAFEKRREDLRRFSEAALSRAKAASVAGAPEVALEVRQGAAVSALDQYVTEHK
                     ADLVVVGTHGRTGLRRAILGSVAESSIARLPCDVLAVRPQSA"
     gene            complement(15778..16965)
                     /locus_tag="VF226_12790"
     CDS             complement(15778..16965)
                     /locus_tag="VF226_12790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7074910.1"
                     /translation="MTDPTESPVSIPIDPEWQELRDGVRRICEGFPNEYWVKLDHESA
                     YPAEFVNALTEAGYLAALIPDEYGGSGLPLSAACAVLETIHEQGCNAAACHAQMYTMG
                     TVLRHGSEEQKRKYLPGIASGELRLQAFGVTEPTTGSDTTQLKTRAEKKGDRYIVTGQ
                     KVWTSRAMQSDLMLLLARTTPADQVRKRSEGLSVFLVDLREAKGRGLEIKKIDAMINH
                     NTCEVFIDNLEVPAENLIGEEGQGFRYIVDSMNAERILIASECLGDAQYFIRRATEYA
                     GERVVFGRPIGQNQAIQFPIARAHAETVAASLVVAKAAALFAAERDCGAEANMGKLLA
                     SEAAWHAGEACMQTFGGFAYAREYGIERKWRETRLYQTAPISTNLILAYIGQHVLGMP
                     RSY"
     gene            complement(17152..17946)
                     /locus_tag="VF226_12795"
     CDS             complement(17152..17946)
                     /locus_tag="VF226_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7074911.1"
                     /translation="MRREYETLLLDEPVAHVTVVTMNRPQALNAMNTQMMADLRDCFS
                     SYYVDQGETRCLVLTGAGEKGFCAGADLKERNGMTDEAWRRQHAIIEQAIRAIMECPL
                     PVIAAVNGAAYAGGCELALACDFIYAAPHARFAQTEVALGIIPGAMGTQNLPRAVGIR
                     RAKEIILTATPFTAEEGLAWGLINKLVPAERLMEDVLATASRIAANAPVAVRQAKRSM
                     DKSQDLDRANGYSYEIEAYNRTVGTEDRIEGIRAFNEKRKPDFKGR"
     gene            18247..18822
                     /gene="speD"
                     /locus_tag="VF226_12800"
     CDS             18247..18822
                     /gene="speD"
                     /locus_tag="VF226_12800"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX7074912.1"
                     /translation="MAYDDTLFQLGMDLTRSSTAQKEDFGTAPGAQIDEQVRGSHEDR
                     KDFFIERDGVRFAGSHLIIDLFGAKRLDDLKHIERTLKRCVEVAGATLLHIHLHHFTP
                     NGGVSGVAVLSESHISIHSWPEADYAALDVFMCGRAKPHLAVDVLKHAFEAEHIVVKE
                     HRRGEELNELSWKAASRRQAPDRQESVRRAA"
     gene            18974..19885
                     /gene="speE"
                     /locus_tag="VF226_12805"
     CDS             18974..19885
                     /gene="speE"
                     /locus_tag="VF226_12805"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX7074913.1"
                     /translation="MKRWIEETFHPHWRVRLEADKILHEVQTEHQHLVIFENAAWGTV
                     LILDGVFQLTTLDEFIYHEMMAHVPLMAIDRPRRVLVIGGGDGGVLREVLKHPSVEKA
                     TLCEIDRNVIDLSLKYYPQVSNGAFDNPRAEVVIADGLKYVAETTEKFDAIIVDSSEP
                     IGPSAVLHTEEFFADCRRALKPHGILVTQNGLPFLCPEAFSNTTRILSGLFNRCAPYL
                     CHQPCYFGGEFAINWASEEPAHLEISDAKLARRQKARGIETRYWTPKFHVAAFALPAY
                     AEKLVNEARAAARGKATKAQPKVSKSK"
     gene            complement(19952..20455)
                     /locus_tag="VF226_12810"
     CDS             complement(19952..20455)
                     /locus_tag="VF226_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX7074914.1"
                     /translation="MTVMRVLIAGILLPLMGSLAFAQGSSDGIAVSEAWLRATPGGVK
                     TGAAYLTISDTGPGDRLVAAKSDVAERVELHNHVHENGVMKMRRVDAIEIPAGETVTL
                     APGGYHLMLFDLKHPLKAGEKVQLTLEFEKAGEIPVTASVEPIGAKGPSGAVAGDHHH
                     SEAGHKH"
     gene            complement(20566..21198)
                     /locus_tag="VF226_12815"
     CDS             complement(20566..21198)
                     /locus_tag="VF226_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074915.1"
                     /translation="MKRWAVAIALLVAAAPLAAADPLDGHDCFSSDNERRIKGCTELI
                     EGAGLTGQMLGSVYAMRALGYSLKGLYDIAIRDYDEAIRLVPDFAVALNNRAWAYFRS
                     GRPEAGRPDVDRSLQLQPTSPHAYDTRAHIRQVTGDPAGALRDYDAAMRFGGERMIQL
                     YQCGLAALGLYGGPIDGAYTADLRRAMQACVVSKDCDPLPPDEECRAVTS"
     gene            21516..22673
                     /locus_tag="VF226_12820"
     CDS             21516..22673
                     /locus_tag="VF226_12820"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074916.1"
                     /translation="MKRADLIALAQSTGFPKVSIYLPTHLSYPEIEQDPIRLSTLLKE
                     ADQKLAACGVRRPEIDDLLAEARGRVPAAMFWRYQDHGLAVLIEKGTTRFVKLPREVP
                     ELVVVGPRYHLRPMIRMFRNGENFNLLAVTREKVALYDGRERELREVTVEGMPTSFAD
                     VARRTEFETTFNYASRDRGDPRSGHESGKFHSLGVSPEDYESIELDRFLLDVAKAVDR
                     HLANSEAPLVLAAQPRIVGHLRKHLDYRYVTQEDIQSDPFALTEQELHAEGWRIAEPV
                     VRADRENARRRLRAYLEGAGVPGNKDLQTLMREAIAGRVECVFLAPDANVWGVFDEEY
                     QVLRIDPEPGPENEDLLNRLAVETLIRSGDVFTLPEDLRYRAGPVAGLYRY"
     gene            complement(22822..23409)
                     /locus_tag="VF226_12825"
     CDS             complement(22822..23409)
                     /locus_tag="VF226_12825"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX7074917.1"
                     /translation="MKNPETEANLKLSFHGMEPSVTIRSKVHEYVNKLQRRYNDITTW
                     HVHVNGPVPGTGQFKVTIEARVPGAEIAVGRKPGDINAHSDALVAVRDSFAALERRLK
                     KRRRQQYGEVKVHEGKPQGQIDIIFYDEGYGFVEVTDGRRVYFHRNAVIDDAFEDLEV
                     GDPVELTINSDDSPRGPQATSLRRIRPMRFNPQAT"
     gene            23648..24367
                     /locus_tag="VF226_12830"
     CDS             23648..24367
                     /locus_tag="VF226_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074918.1"
                     /translation="MTGEGKVVIVTGAARGIGAATAVRLAREGWKVVLADIDGAALAN
                     VAKPANGYAVVCDVGVEADVQRLVDESVDQFGRIDAIVSNAGINLRGPLSELTLDSWN
                     RVLAVNLTAAFLLARAAERPLRDAKGAMVLIASTRAHMSEPDTLPYSASKGGLVALTH
                     ALAMALAPVRVNCISPGWIDTGKSGPLSPEDHAQHPVGRVGQTDDIAAAVSYLLSDGA
                     GFITGAELIIDGGMTRKMIYV"
     gene            complement(24371..>24712)
                     /locus_tag="VF226_12835"
     CDS             complement(24371..>24712)
                     /locus_tag="VF226_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7074919.1"
                     /translation="VAGVRGSKGRSAYGASKGAVIVLTQVMANDLARHGIRVNAVAPG
                     PIDTPLVRDLHTAEDRETWTRFIPMRRYGRPEEIAPAICFLLDDRQASYLTGEVVAVD
                     GGFRGAGIIAG"
BASE COUNT         4577 a         7714 c         7932 g         4489 t
ORIGIN      
        1 ctcaacgccg ccgacaggtc ggcgggcagc gaggcggatg tcgttcctgc agacgggtcc
       61 gcggagctca tcatcgacgg cggcgaattc cagattgctg gtcctcagga tcaagctcag
      121 gccggcgatg ccgggcccgc ggcaccgctc atcaaggctt tacgcgcgct gaacttcgat
      181 accgtcgtca ttcgccaagg cactgttcgg cttgctctgc cggatggtcg catcgagacc
      241 ttgacgaatg tccttgcgga agtgcgtcgc gatggccgaa gcagcatcgg cgctcggggg
      301 accgcgagat ttcgcggcca cgaattctca ttcgatctgt ccgcgccggc cacggccaat
      361 gcgagcgccg ttgcaggcgt gccgatgatg ctgcgcatca agtcgccgct catggatgtg
      421 gccttcgacg gcggaatcgg agcctcggac aggctccacc tgcatggccg catcgagttt
      481 gccgtgccga atgtccgcag cgcagcgcgt tcgttcggtg cgccctggcc ggatgggcct
      541 ggactccgtg atgtcgcgat caaaggcgat ttccattggc aaagcccggc gctcacgttc
      601 gacaaggcca gcttccgcat ggacggcaac gaagcgacgg ggacgcttgc actgagtttc
      661 gccggggagc ggccggccct gaccggcacc ctggccctgc aatcgctcag tctggccccg
      721 tacatctcgc cggatggagc caccgacaac agcttttcat cctttctcgc ctgggcgcgg
      781 tcggatcgga gcgagctgtc ggctcccatg gcccggtatt tcgacgcgga cgtgcgcttg
      841 tcggcggggc agctcgtagc cggcggaacg accttcggac gtttcgcggc gtcgttgtcg
      901 ctcaagcggg gccgtctcct cgctgacatc gccgagatcg acctgactgg cggccgcggg
      961 agtgggcaga tcacggccga cctcgccggc acccttccgc agatctcagt gcgcggcaag
     1021 ctggaggaca tcgatgccgc acgcgcctcg atggccctta tgggtcacag cgctgtccaa
     1081 ggcctgtcga cggtgagcat ggatctgaca gcagaaggaa actcattgtc cgaattgctg
     1141 agtggagtcc gcggaaagat tgcattgaat gtctacgagg gcgggcggct cggcatcgac
     1201 atcagggcgc tcctcgacgc cgcgcagaag ggcgcgctcg agggctgggc tgttggccgc
     1261 ggccagacct ctgtcgaggg actccaggcg aagctgcgcg tcgacaaggg aatactcgcc
     1321 agcgagctgg tcgaggcgac cgctgggaac agcctgctcc gggccttcgg taccatcagc
     1381 cttcagtcgc gtcagatgga tctgcggctg ctgctcgacg ggttgggtga ttcgaagccg
     1441 gatgtcgcag gggatgtgct cgtgttccgc ggcccctgga catcaccgag catcacgctg
     1501 cagcgatagt tccgccccag ttcatcggag cgcgaccggc cgcgggccct tcgcacccgg
     1561 cggccggcct cccagttttg ctagaaccgc cgccgcgggc ccgtgtcgat atggaacagg
     1621 ccaccgccat agtgcttcag tccaccgacg gccgagcgca ggtaggcgag cgctgagcgg
     1681 acgttgccga atacgcggat atccgctgca ttcccagtca gatggtacga cctcggcgca
     1741 ccgccgaccc ggcgattgtg cttgcggctc cggcaggtcg agttgacgcg gaccttgccg
     1801 tagttcgccg cgacgtgctc gatggcggcg cggagctcag cattcaggca gctcgcgctg
     1861 gccttccagt ggatcgtgcc gccgccggtc gcactggggg gtggttcggt gctcgggatg
     1921 ggcatggggc cgaggctcgc agtccgcacg cgtttcgtct tgcgggggcg gcggcggtcc
     1981 gtgtccggca tgtaggcgcc gagcctgctc ggctggtcga acctgactgc ggatggcgac
     2041 ttggcaaacg ccgccttcag ccattcgttg ccgctgtctg cgcggctttc cgccggagcg
     2101 cacatcatgg ccgccgcgcc gaggagaagc gctggcagga tcttgttccc ggcacgcgcg
     2161 cgcacgcctg caccggcctc gcacgcgagg ccgcgcacca aactcacaat actcattgtt
     2221 cagatgtccc ccggactgcc cgtccgcgcc tgatcagccg cttaccccat accgggccgt
     2281 catcacctca tgtgatcaac gggaaaacta ttcgcggagc agacgtgcga cgggcgttgg
     2341 ctcaatccaa ttcattaggg ttgcagattg ccaaaatcag cgcgcgaccg atttttcatg
     2401 agcagggccg catattgcgc tgacgcagtc cggcccgctt gggtgcgagg cggatctgcg
     2461 ggggaaagaa ttggcgaaga acgccgcaca ggctcaagcc agcgtccccc ctggcggggc
     2521 agaggcgtaa tcgtcagatg tggccggaat taggccgggg tctgatgtga aatccgggcg
     2581 cgattaccaa gggttaggtt aacaggcaga gagatcgcgt caatctcggc agtcgttgtc
     2641 agaaggcagc tctgctcggg agaaatcccc agaacagcgc catcaccagc aaggtggtcg
     2701 ccaaccaggc tgaacggagg gcgaagtcca ggaatgcttc gcgccgcgcg gtgctgatga
     2761 tgctctgcgc caccgccgcc tgcggccgct cggagggatc gagcatcagc cagagctcgg
     2821 tgcgctggta aggggtcgaa gctgcccgcc agctcatgcc gatcagcaca aggctcaaca
     2881 gcaggcaacc aaagccgccc gcgcgcaagg caagcgcaaa ctgggaggaa agcccgacca
     2941 tgaatgtcgc gatggccagc attgcaaagc cacaggcgcg ggccacggaa acatgggcga
     3001 gctggttgat cttctccata gccgcattct cacgtcgaga ttcggtttcc ccacccgaca
     3061 cgaatacgac tttagtctta tcccgttccg cgtgcgacgc aagataccgg gctgcgtctt
     3121 acgagcgccc ggccaggctc atcagcgtgg gcagcgccag gatgagcgtg gcgccgagcc
     3181 aaatcccaag cgaggttgca ttgctctcgt gaagaccgcg gaagcgctcg tagagcgcgg
     3241 ctggaacgcc ggcgatcagc agggtcatca gcgagatgaa aatcgacgtg atgtagaaaa
     3301 gcacgagagg agtagacagc agcgttcccg caatgaacgg cctcagcaga atgaagaccg
     3361 ggtcgaaata gggagagaag tgcatgccat tgagcaggga cacggctgcc gcaccgacga
     3421 gcagcatgtc acgcttgagc aggtcttccc ggttatccgc accagtcatg cgctataccc
     3481 ccacgtccgc cgacattcct gcggccacac aggtgaggaa ccagaacatc cgggccgcca
     3541 tgagccaagc gattgcgaac accatgacga gaccgttcgg cacaatggcc ggcgtgacca
     3601 ccctgatcgc cttgagcacc ggatcggtaa tcgactgaaa cacacggagc atcaccgcgt
     3661 ccgggttctt gcggaagaac agcgtcagca gatagcgccc gatcaacgtg taaatgagcg
     3721 cggcgagaat gagattcggt atatggaagt accagtacgt aaagaagctt tccgcgaccg
     3781 acattgcacg atccgatttc cgcgccctca aagtttttgt ggcagacaag aagggcgggg
     3841 cgcaaggccc cgccctccat tcattcttgg cagcgcgcaa gtctgccgtc agtgggccac
     3901 cgccggggcg tccttgtcgc gaagaatggt ctggccgcgg ggccgccgcg tggcgtcgac
     3961 catctcctgg acctccttgg atggctcggg tgtcagcagc gacacgatcc agatcgccag
     4021 gaagccgacg ggcaggccaa agatggccgc cgagatgttt ctcacacccc accagttcgc
     4081 gatcgtttgc gcgtgcttgt cgagcgccac caacgccgcg tccctggctg ctccgtccgc
     4141 tgcattcatc caggccgcct tgagctcgtt gaacttggcg atggctgccg gcgaagcgtt
     4201 cgagagccag ctccaggtct cgtagaagtg cacagcgaag tagcgtgtgc cgaccaggta
     4261 gtagaggcat atcccgaagc ccaggaccat tccgacgatg caaccggccg tattggcccg
     4321 tttccaccag atgcccagca ccagtgccgg gaagatgccc gcagcggcca atgagaacgc
     4381 ccacgccacc atggacagga tgtctgccgg tttcgtggag gcggtgtacg cagcggcaac
     4441 ggccaccgtc accagcagga tgcgagcgac gatcaggcga cgcgtcgtcg gcgcgttgcg
     4501 atcgagcatc ttatagtaga tgtcgtgcga caacgcgttg gcgatggcga gcagcagccc
     4561 gtcagctgtt gatagagatg cagcaaggcc gccggcagcg acgagaccgg cgatcacata
     4621 cggcatgccc gcgatctccg gcgtggagag cacgatcacg tccgtattga tgctgaagtc
     4681 ctgcaggcga atgacgccag ggtggcctgc aatttgggaa caagccgcct tgatcgccgc
     4741 gacgtcgatc gcatcggccc cgcagatctt caccagcccc agctgcccgt acgtgaagag
     4801 ccacggccga atgctgtcaa ggtcgcgacc aatgacggtc gagtacacct ccagcttcga
     4861 aaaggctgcg tatgcaggtg ccgtgaagta gagcaggaag atgaagaaca gcgaccaggc
     4921 caccgacaac cgagcttcac gcacggatgg cgtggtgaag tagcgcatca ggatgtgcgg
     4981 tagcgacgcc gtgcccacca tcaagcacag gatcagcgcg aagtagttga gcggatcata
     5041 ggtcgtgaag ggctggatgt ggggcttcag cgattgtgcc gtcgccaacc cgctcgtcag
     5101 catgttctgc tcgagcgcgc cgatatcgcg gagggcctgc ccataggtga gctgaggcac
     5161 cgggatgccg tatatctgcg tcgacaggat gatgacggga atcaggtagg cgacgatcag
     5221 cacgatgtac tgcgccacct gtgtccaggt caccgctttc ataccgccaa gcatcgagca
     5281 gagtaggatg ccgaccaggc cgacatagac cgcgaccgtg aagtccatgc cgaggaagcg
     5341 ggaggcgatg atacccgtcg cgtagacctg cgccgtaaca taggtgaagg agcagcagat
     5401 cagcacgatg acggcaagga aacggctgaa gtttccgccg taccgctcgg agaagaagtc
     5461 gggtaccgtg aatgccccga acttgcgcag gaacggcgca atgagaatgg cgacgagcac
     5521 ataacccccc gtccagccga gcacgaaggc caggccatcg taacccagaa ggaacagcgt
     5581 gccggccatg ccgacgaagg atgcgcccga catccagtca gcgccggtcg ccatgccgtt
     5641 atagacggcc ggcacacgcc ggccggcaac gtaatactca ccgacttccg tcgtgcgcga
     5701 aatgatgccg atgacggcat aaacggcaat cgtcatgaaa acgaagccgt agccaatcca
     5761 ctggttggga acgccaagct gctcgagaat ggcgaggaag atgacgaatg cgaagaaacc
     5821 gcctgtatac aggccataaa tcttgccgag attggaaatg aaatcccctc tcgcggctgg
     5881 tgcttgatca gccatggcgc gctcctcact catcttcagc cacaccttct tcgcgatcga
     5941 tcttgtcctg cgcccaggcg tagaagaaga gcatcacgac gaaggcgatc agcgaaccct
     6001 gcgcggccat gtagaagccg agcgggaagc caaggatctg aatctcattc agcggattca
     6061 caaaaaagtg gatcacaaaa ctgaagaagg cccacagcgc gagcatggtc cacataaggc
     6121 tactggttcg tcgccagtac ctgtcttcgc cctgttccat cagtcgtttc tcccaaaaag
     6181 ggcgcccaca gagcgcccat accaatccaa ggggagagca gactttggac ttgcagtgtc
     6241 tccccgtatg ctgaaggtta agggcttgct aactgaatcg taaccctgac tttaggcgga
     6301 tccgctgcca aagtgttgcg acgatgcaac agggggatcc ggggttcttc cggcggaaat
     6361 ggctgacagc tgccatgcaa ggtggcactg tgaccgcgcc caagtaaggc tcccattcaa
     6421 gttcattcgg cgggctcgcg gatgccaaat tcgagttttg cgacgccgct ggtttcgctc
     6481 tccgcggtcg tcctcgattt ggagacgact gggcttgacg cgcgctcggc acgggcggtt
     6541 caggtggggg cggttcggat cgacgaggga cgtatcgtcg aagatgcaaa gctcgaccgt
     6601 ctggtgaatc cggggattcc gattcctcca gcggcgactg ccattcacgg cattaccgac
     6661 gcgatggttg ccgatgcgcc gcgctttgcc gagctgctgc cggaaatcga ggcattcatc
     6721 ggtgacgcga tcgttatcgg ccacaccatc ggctatgacc tcagcgtcat gaggcgcgaa
     6781 tatgagctgg ccaatcgcgc ctggcggcag ccgcgctccc tcaatctgcc cacgttggcc
     6841 aagcttgccg cgccgaccct cgcccaccac agcatcgacc acctttgcaa ctggctcaac
     6901 gtgccgaaga cagggcggca tacggcactt ggcgatgccg aaacgacggc ccggcttttt
     6961 ctggccctca tcccgctgtt acgggagcgc aatatacgga cactggccca ggccgaatca
     7021 gcgagccgcc agctggcgga ggccgaggcg cgcgcaacgg gcgggctcct tccttctcca
     7081 ggtgttgcga tcagcccgca ggagccgcgg ccgctcgccc gtatcgacag ctttccttac
     7141 tcgcacctcg tgcgcgacgt gatgagcgcg ccgcccgttt ttgcagatgc ctctgcaagt
     7201 gtgcgggaag gcatccaaat tcttttggac aagaacgtca gctcggtttt cgtccgaaac
     7261 aatgccggcg agctcgggat cgtgaccgag cgcgatttgc tgcgcgccat caataaggac
     7321 ggtgatgcgg gattggacgc tccattggaa gccataatga ccaagccgct ccagaccgtt
     7381 ccagaggatg ctttcgtata ccgcgccctc ggccggctgg agaggatggg cttccggcac
     7441 ctgggcgtgc acgatgccca cggagagatc atcggtgcgc tgacgagacg caacctgctc
     7501 cgccaccgca gcacatcggt gatcatgctg ggagacgagg tcgacagtgc gacctcggtc
     7561 gcggcgctcg gtgctgcgtg ggccaagctg ccggctgtgg cacgcagcct cgttgccgac
     7621 gacgtcgatc cacggaccat atcagctgtc atcagctcgg aaatcagggc catcacccgc
     7681 cgggcagccg agctcgccga ggccaagctg gcggcggaag gccagggccg ggcgccgtgc
     7741 gcctttgccg ttctcgtgct cggctctgcg gggcgcgggg agagcctgct ggcagccgac
     7801 caggataatg cgattgtcta tgaaaccggc gccgagggcg gggcggagga cctctggttc
     7861 gaggcgctcg gcaaggagat gtgccagact ttgcacgagg tggggattcc gttctgcaag
     7921 ggcggcgtga tggcgaagaa ccgcgcctgg cggctgagtc tttccgattg gaaggcggtc
     7981 atcgatagct gggtgcatcg ccagcgcccg gaggacctgc tcaacgtcga catctttttc
     8041 gacagcgtgc ccgtccatgg cgatgccaca ctcggcgagg cagtttggaa ctatgccttc
     8101 gagcgcgggc acagcgcgcc cgatttcctg aagcttctca tcgaggtggc gcggcagcgt
     8161 tcgccagcat tcacgctctt cggcaacatc cggatcgatg agaagggacg catcgacctc
     8221 aagagggccg gcctgatgcc gatcttcacc tgcgcacgcg tgctctcgat ccggcacgac
     8281 gttcgcgcgc gttccactcc ggaacggctg gagggtgtgg cggcacgagg catcggctct
     8341 ccgcacgaga tcgaagaaat catcgaagca caccgcacca ttctcggcgc catgctcgcc
     8401 cagcagatcg acgatgccga gcatgggatg agcttgtcct cgcgcgtcaa tccggatcgc
     8461 ttgggcaagg cgcggaaacg gcagctttcc aaggcactcg gcaaggtcga tgccgtgatc
     8521 gacctcgtca gcgaaggacg cctctgaata tcgccggacc cctgtccggc actctcttgg
     8581 ggccgtctgc tggcggattc ggccgcgctg gaacccaaca gtttggatga ggttatccgc
     8641 gggccagtgc ccattgggag ttgacgcgtg caggtcctcc gcacggacgt tgccatcatc
     8701 ggaggtgggc tcggcgcttg cgctgctgcg cttgccgcgg cgaggctggg ctgccgagtc
     8761 atactgaccg aggaaacaca ttggatcggc ggtcagctga ccaaccaggc ggttcccccg
     8821 gacgagcatc cctggatcga ggaattcgga gcgacggcga cctaccgggc gctgcgcaac
     8881 ggcatccggc agtactatcg cgatcatctc ccgctgacca ctctggcgcg ggcgtcagag
     8941 cacctcaacc cggggaacgg ctgggtcagc cgcttgtgtc acgatccgcg cgtcgcagtg
     9001 gccgttctcc accagatgat catgccgtat caggccaacg gcaagatgtt cgtaatgcac
     9061 ccgcagcgtc agctcgccgc gtggacccac ggcgatcgcg tgacgggtgt cgtcgtccgc
     9121 gggctcgagt ccggccgcga cactctgatc gaagcctcct atttcatcga tgccacacct
     9181 cagggagagc tactcgacct cgccggcgtg gagcatgtgt gtggcgccga gtctcgcgcg
     9241 gagaccggcg agccgcacgc tccggagatg gccgacccga gggaccagca ggccattacc
     9301 gtgtgcttcg cgatggagta catcccaggg gaggatcaca cgatagacaa gccgcgccag
     9361 tacgagctgt ggcgcagcta tcagccgccg ggctggcctg gacccctctt ggggtggacg
     9421 acgcagaagc cggaaaccca cgaaccgatc actcggtacc tgttcgaggc ggaagacggc
     9481 aagccttggt ggaatttccg cagaattctc gatgtttcga acttcgagga aggctttggg
     9541 gcccatgaga tcagcgtcgt caattggccg caaaacgatt actggttcgg cccggtctgc
     9601 gacgtcgacg aggtcgagca ggtgcgcaac attgaagcgg cccggcagct cagcctcagc
     9661 ctgctctatt ggcttcagac ggaagccccg cggcctgacg gcggcgtggg atatcgaggg
     9721 ctccgccttc gcggagacgt ggtcgggggc accgtcgacg ggctcgcgca ggcgccttac
     9781 atccgcgagt cgagacgcat caaagccgag ttcacggtgc tcgagcagca catcgcctat
     9841 ccgctgaggc cagacggacc cgagtttttc gaagattccg tcggcatcgg ctgctaccgg
     9901 atcgatctcc acccgagcgt cagcggcaag ggctatttgg atctcgggtg ttggccattt
     9961 cagatcccgc tcggctcgct gatacccgtc agggtggaga atctgcttcc gggcggcaag
    10021 ggcctgggcg ttacgcacat cacgaacggc gcctatcgcg tgcatcatgt ggaatggaac
    10081 gtgggcgagg ccgcgggcct gctcgctgca ttctgcctgg agcgcaaggt cacgccgcgc
    10141 gccgtcagaa acacgaaggc gctgctcgag gatttccaat ccttgctccg caatcagggc
    10201 attcccctca cttggccgtc gcttacgccc ttgtgatctt ttgattccta acccgtggtg
    10261 gccggcatgg aacagcagga tcaattgttt cgtatcggta tttccggatc ctacggggga
    10321 atgaatcttg gcgacgaggc cattcttgac ggcattttga gccagttgcg gagcagccta
    10381 tcggccgaga taaccgtttt ctctcaaaac ccgaaagata ccttgagccg gcacaaggtc
    10441 gaacgcgcgg ttccctgccg ggatatgacc cgaaaagaag tcgcgcgcga aatcgaaaga
    10501 ctggaccttt ttgtcctcgg cggaggcgga attctctacg atcgcgatgt tcaccgctac
    10561 ttgagagagg tgaatatcgc gcacgaattg aaagttcctg ttttcatata cgccgtaagc
    10621 gcaggcccgt tgacgctgac cgccacgcgg aacgcggtgc gcgacgctct caatctcgct
    10681 gcagtgatta ccgtccggga tcgacagggc tacaggttgc tcgaggacgt cggcgtcgat
    10741 cgggaaatcc atttgactgc tgatccggcg ctcctgatcg acccggagga gattccacga
    10801 acactattaa gggccgaggg cgtcgagttc gagcgcccac tggtcggatt ttcggtacgc
    10861 gagccgggcc ccgcagcccc ggacatcgac ccgcagcact actacgcgct gctcgccaat
    10921 acggccgatt tcatcgtcga tcggcttgat gccgatgttg tcttcgtgcc catggaacgg
    10981 accgatgttc agcacagcca tggggtcgtc gcgcagatgc acaggtcgga gcgggcagag
    11041 gtgttgaggc gcaagtatac cgcgcaccaa accctgggcc tcatggatac ctttgagttc
    11101 gctgtcggaa tgcggctcca ctttcttatt ttcgcggctc ttcgggggac gcctttcgtg
    11161 gccctgccgt acgcatccaa ggtgatggga ctcgtcgaag acctcgaaat ggatattcct
    11221 cctctgggcg acatcgggac cggtcagctc atcgccatga tcgaccgatc ctgggatacc
    11281 cgggatgaaa tccggcataa gataaaatgc tgccttccgc ccctgcaggt gcgcgctcga
    11341 aaaaccaatc agcttctcgt cgacctgttg cgcaagcaga gtggctccgc cggtctcaaa
    11401 tcggcaacgg cttgacggct tgggccatca aacgtcgaag catgcctcct tcgctttgag
    11461 cagggctacg acgacagaat tgaccggggt cgccacgccg acttttgcac cttcgcgcgg
    11521 gatggcgccg ttgatgttgt cgatctcggc gcggcggccg gccatgtggt cgagcagcat
    11581 ggaggggcgc gcgttgggaa ttttctcgcc gaatgcgcgc acgtatgcga ccggatcggt
    11641 gaagtcgacg gcgatgcctt tggcgcgggc gacgagatag gcctcgttcg cacaggcagc
    11701 gcatatgctc caggcctgcg ggttcgcctg cgcctcgccg acgcggagac ccgtcaaggt
    11761 gcacggcccc gaataggcga cgttacagat cagcttctcc cagaccatct tgtggatgtc
    11821 gtcgaaggtg agcacattga agccgccagc ccgccaggcc tcggcgacgg ccgccagccg
    11881 cggcgtttgc cctccatcca tctcgccgag gcgcaagaac tccatgccgt tgtgatgggc
    11941 atggcccggg gccttgatgg aggcgccaaa tccgccgacc acgcccatca tgatccggtt
    12001 cgagccgacg atttcggcca ccttgtcggc gctgccgagg ccgttctgga tcgtcaggat
    12061 cggcgcgtcg gctttcgcga tccgaagcgc ggcgtgggcc gcatcgacga cgccttggtc
    12121 cttggtcgca attacaacca gatccgcatc gcgtatgtcg gccggatcgg tcgtggcatt
    12181 taggcgcacg gtgcggtcgc cggaagcccc ttcgacgcgc aggcctttct cgcgaatggc
    12241 atcgatgtgc tcctgccatg tgtcgagcgc ccagacatca tgcccgccgc ttgccagcag
    12301 ggctgcatag accgagccca tcgcaccaac gccgatgatt gcgattttca tggtattcat
    12361 cctccttgat gcgacgaaaa tgttgggcag gcttcggccc caagtctgca tgatcgaggc
    12421 tggagctgga agaggctggt gagggggctc gtgtgtgcgg ggctcccacc gaggtagcgg
    12481 acgagacaat gagagccggg taccatcttg ttcggaggct ttggagcact gcaccggatt
    12541 gcgcttactc cgggacgcgc cgcagcgcgc cagtgcggtt tgccctgtcc attgtcagcc
    12601 tgctcgctgg gctcgtgatt ttcctcggcc agccggtcaa ggggcaggat gcgggtggtg
    12661 gtccggtgct cgtgttcgac gtcaaaggcg tcatcggatt tacctcggca cagcacttgg
    12721 agaggagcgt cgagcgggcc aaggccgaga aggccagcat catcatcatg cgcatcgaca
    12781 caccgggcgg gctcgtctct tccacacgag atatgatcca gacgatcctc gcgtccccca
    12841 ttccgatcgt tggctacgtc gcgccgagcg gggcgcgtgc cgccagtgcc ggcacctacc
    12901 tgatgtacgc ctcgcatctg gcggcgatgg cgcccgcaac acaccttggc gccgcgacac
    12961 ccatccagat gggcgctccc ggcatcacac cgtccccgag cgagaagaag gaccagccct
    13021 cggaaccgga cgctgcagag cgcaagctca tcaacgatgc ggtcgcctac ctgcgcggcc
    13081 tggcggagct gcggaaccgg aatgccgact gggcggagaa ggccgtgcgc gaaggcgcga
    13141 ccctgacgtc gacgaaggcc ctggaggaga aggtcatcga ttttctcgcc ccggacctga
    13201 gagaggtgat cgacaaggcg cacgggcgca cggtgacgac gagcgccggg accgtgacgc
    13261 tcgacacgcg aggcaagcgg atcgtctcgg tcgagccgag ctggcagatg caggtcttgg
    13321 aggtgatcgc cgatcccaac gtcgccttca tcctgatgct gctcgggatc tacggcatca
    13381 ttttcgagct tctgaacccg ggagcattgt ttccgggcgt catcggcgga atctgtctcg
    13441 ttctggcgct gacggcgctc acggtgctgc cagtcaatct gggcgggatc gcgctcctcc
    13501 tccttggaat cgcgctgctg gcggccgagg cgttcgcgcc gggctttggc gtgctcggca
    13561 tcggcggtat cgtggcgttc atcctcggcg gcctgtttct attcgatccg gaccaggtgg
    13621 acatccagtt cgaggtctcc cggcctctgg tggtgggcgc cgccatcgcc agcgccatca
    13681 ttcttgccgg cgtcctagga atggcgatgc agtcgcggaa gcgagaggtt cagacgggtg
    13741 gcgagcagct gatcggcagc acggccgaag tcgtggaatg gtcgggtcac gcggggcgcg
    13801 tgcgcgtgct cggagagatc tggttagcgg agtccgacgt ggagctggcc cgcggagacc
    13861 gggtgaaggt caccgcgcgc gaaggtctga agctgacggt gaagcagaca acctgacggg
    13921 aggggaggag agccgaaatg gacgcgctga cgacgagcct cattccaatt gtcatcatcc
    13981 tgctgatcct ggcatcggcg atcaaaattt tgcgggagta cgagcgcggc gtcgtcttca
    14041 cgctcggccg gttctggggc gtgaagggcc cagggctcat catcatcatt cccggcattc
    14101 agcagttcgt gcgtgtcgac atgcggacgc gcgtgctcga cgtgccgccg caggacgtca
    14161 tctcccgcga caacgtctcg gttcgggtga atgcggtgat ctatttccgc gtcatcgacg
    14221 ccgagcgggc ggtgatccag gtcgaggagt tcgaatcggc gaccagccag ctcgcgcaga
    14281 cgacgctgcg ctccgtggtc ggacagcacg atctcgacga gatgctggcg gcgcgggaaa
    14341 ggatcaacaa ccggatccag gacattctcg acgagcagac tgatgcctgg ggcatcaaag
    14401 tttcgaacgt cgagttgaag catgtggatc tcaacgagac catggtgcgt gccatcgcca
    14461 agcaggccga ggcagagcgt ctgcggcgcg ccaaggtgat cgatgcggag ggtgagctgc
    14521 aggcatcgga gaagctcagg caggcgggcg acgtgctcgc gcagtcgccc tacgcgctgc
    14581 agctcagata tttgagcacg ctgatcaaca tcgccgggga acgcagctcg accatcgtct
    14641 tcccggttcc catggacctg atcacgaagt ggtctcaggg cgggcagggg gccccgccga
    14701 agagcgaagc gcaatagctg tcggacggca gctcgaggtc aggcgctctg tggtcgcacg
    14761 gccaacacgt cgcatggcag gcgagcaatc gagctttcgg ctacgctgcc gaggatggcg
    14821 cggcgcaagc ctgtgcgccc gtgcgtgccc accacgacca gatccgcctt gtgctcggtg
    14881 acgtactggt ccagagcgct caccgctgcc ccctgacgga cttccaacgc aacttcgggg
    14941 gcgccggcga cggaagccgc cttggcgcgc gataaagccg cttcggagaa ccgtcgcaga
    15001 tcctcgcggc gcttctcgaa agcctcctgg ctcgcatcgg cgccggacag aaagcccgag
    15061 aacgagctct ccacggcatt gagtagcgtg aacttggccg aagggaaggc ggcgtaggca
    15121 aacgccaggg cctccgccga ggcctcggag aaatcgacgc caacgacgat gctcttgtaa
    15181 gaattctccg gctcggattt gacgacgaga acgggccgat tgccaaaccg gagcacgcga
    15241 tcgatggtcg agcccagcca ctcgtcgccg atcatcagcc tgtgatggac gccgcaaatg
    15301 atgaggtctg cattcgcggc atccgcggtc tggatgatga tgtcgtagat gctgcccgcc
    15361 tcgatccgaa cctcgacgga gaaatcaccc cgggccgcgc cctcccggac ctgcttcagg
    15421 atcgcttcct cgtcgaggcc gcccttgacg gcgatgttca cgttgaattc gccggccgcc
    15481 atctcctggg agacctgttt ttccagcgcc gccttggcac tcgtcttcag cgcgtcggcg
    15541 taatcagggt tcagctcgtc ggggatgacg tgcaggactg tcagaagtgc cttgttggtc
    15601 cgtgccagct cggctgcacg atgaaatgcc cgatcggatc gcgaggacag atcggtggcg
    15661 agaagcaggc gctgcaatcg ggcaggttct tgcatcggag ggaatctcct gcggtctcac
    15721 aatgcggttt caaagaggac gtagagccta gaccagccgc agcaaggtgg ccaccgctca
    15781 atacgagcgc ggcatcccaa ggacatgctg gccgatatag gcgaggatca agttggtcga
    15841 gatcggtgcc gtctggtaga gccgcgtctc ccgccacttg cgctcgatgc cgtactcccg
    15901 cgcgtaggcg aagccgccga aggtctgcat gcaggcctcg cccgcgtgcc aggcagcctc
    15961 ggatgcgagt agcttgccca tgttggcctc ggccccgcaa tcacgctcgg cggcgaacag
    16021 ggccgcggcc ttggccacaa ccaggctggc agcgacggtc tcggcatgcg ctcgcgcgat
    16081 cgggaactgg atggcctggt tctggccgat cgggcgcccg aagacgacac gctcgccagc
    16141 atattcggtg gcgcgccgga tgaagtactg ggcatcgccc aggcactccg aggcgatcag
    16201 gatgcgctcg gcgttcatgc tgtcgacgat gtaacggaag ccctgaccct cctcaccgat
    16261 gaggttttcc gccggcacct cgaggttgtc gatgaacacc tcgcaggtgt tgtgattgat
    16321 catcgcgtcg atcttcttga tctcgaggcc tcgtcccttc gcctcgcgca ggtcgacgag
    16381 aaagacggag agcccctccg agcgtttccg cacctgatcc gccggcgtgg ttcgtgccag
    16441 cagcagcatt aaatcggact gcatggcgcg gctggtccag accttctgcc ccgtcacgat
    16501 gtagcggtcg cccttctttt cggcccgcgt tttgagctgc gtggtgtcgc tgccggtcgt
    16561 cggctccgtc actccgaagg cctgcaaccg caattcgcca gaggcgatcc cagggaggta
    16621 cttgcgcttt tgttcctccg acccgtgccg cagcaccgtg cccatcgtgt acatctgggc
    16681 atggcaggca gctgcgttgc agccctgctc gtgaatggtt tcgagcacgg cgcaggcggc
    16741 gctgagcggc agcccggagc cgccatactc gtccgggatc agcgcagcga gatagcctgc
    16801 ctcggtgagg gcgttcacga attcggccgg gtaggccgat tcgtggtcga gcttcaccca
    16861 gtattcgttc gggaacccct cgcagatgcg gcggacgccg tcgcgcagct cctgccactc
    16921 cggatcgatg ggaatcgaga cggggctctc ggtgggatct gtcatgcctg gtacaccttg
    16981 ctcgaaagct gtggccgcac gcgcttcgtg atgagatcta tggtcgaccg tggctctcta
    17041 gtccgtcatc ccggtgcttg tcaccgggat ccactgtact gctcgctccg gcgctcgtgg
    17101 ttggttggat cccgggcaca aggcccggga tgacccttac ccgctggacc gtcaccggcc
    17161 cttaaagtct ggcttgcgtt tttcgttgaa ggcgcggatg ccttcgatac ggtcttccgt
    17221 gccgacggtc cgattgtagg cctcgatctc gtagctgtag ccgttggccc ggtcgagatc
    17281 ctgggacttg tccatggacc gcttggcctg acgcaccgca accggcgcat tcgcggcgat
    17341 ccggctggcg gtggcgagga catcctccat cagccgttcc gccggcacca gcttgttgat
    17401 gaggccccag gccaggcctt cctcagccgt gaacggggtg gcggtcagga tgatctcctt
    17461 ggccctgcgg atgccgaccg ctcgcggcag attctgggtg cccatcgcgc ccgggatgat
    17521 gccgagcgcc acttccgtct gggcgaaccg ggcgtgaggc gctgcgtaga tgaagtcgca
    17581 cgccagcgcc agctcgcagc ctccagcgta agctgcgccg ttcaccgccg cgatgacggg
    17641 gagcgggcat tccatgatgg cgcgaatggc ctgctcgatg atggcgtgct gacggcgcca
    17701 ggcctcgtcc gtcatgccgt tgcgctcctt caggtcggcg cccgcgcaga agcccttctc
    17761 gcctgccccg gtcagcacga ggcagcgggt ctcgccctgg tcgacatagt aggagctgaa
    17821 gcagtcccgc agatcggcca tcatctgcgt gttcatcgcg ttgagagcct gcggccggtt
    17881 catcgtcacg accgtgacat gcgcaacggg ctcgtccagg agcaaggtct cgtattcgcg
    17941 ccgcatctgg tctcccctga atgggactgg gtgcgtcacc ttgatacggg aaacttgccc
    18001 acggagctag cgccagcgac ttgccaaacg cggagcggcc gcctcgaatc gatcgtccta
    18061 agggaaagta aacgcgcgcg gcaaaaaatt gcccggtaag gcaattttgc acttgcaccg
    18121 acgggacata ggcctcatat cgggcttctc cgttcgtccc cctgccccaa aggtcaagga
    18181 gcgtcctgaa accctaaagg taaggagcgg gcgggtcacc tctgtctact ggttggggag
    18241 ggtcgcatgg cctacgacga caccctcttc caattgggga tggacttgac tcgttcaagc
    18301 accgcccaaa aggaagactt tggaaccgcg cccggtgcgc aaatcgacga acaagtgcgc
    18361 gggtcgcacg aggacaggaa ggacttcttc atagaacgcg atggcgtccg gttcgccgga
    18421 agccatctga tcatcgacct cttcggtgcg aagcggctcg atgatctgaa acacatcgag
    18481 cgtacgttga agcggtgcgt ggaagtggcg ggagcgacgc ttctgcacat ccacctgcat
    18541 cacttcacgc cgaacggcgg cgtctcgggt gtggcggtcc tttcggagag ccacatcagc
    18601 atccatagct ggcccgaggc cgactacgcg gcgctcgacg tgttcatgtg cgggcgagcc
    18661 aagccgcacc tggcggtcga tgtgctgaag cacgcgttcg aggccgagca catcgtcgtg
    18721 aaggagcaca ggcgaggcga ggagctgaat gagctgagct ggaaagctgc gtcccgccgg
    18781 caggctccgg atcgccagga gagcgtgcgg cgggctgcct agcgcagtca caccgacaat
    18841 tcgcttcagt gaggcgcgta ccggctcacc ggccgtgcgc gcctcattgg ctgttgaggg
    18901 tgccagattt caggcccctg ctcgggagtt ctctggcagg cgaaagcctt ggaaaccgcg
    18961 aggaggttga ccgatgaagc gctggatcga ggaaaccttt catccgcact ggcgtgttcg
    19021 tctcgaagca gacaagatcc ttcacgaggt gcagacagag caccagcatc tcgtcatctt
    19081 cgagaacgcc gcctggggca cggtgctcat tctcgacggc gtcttccagc tcaccaccct
    19141 cgatgagttc atctaccacg aaatgatggc gcacgtgccg ttgatggcca tcgaccggcc
    19201 gcggcgggtt ctggtcatcg gcgggggaga cggcggcgtg ctgcgcgagg tgctcaagca
    19261 cccgtcggtc gagaaggcga cgttgtgcga gatcgaccgc aacgtcatcg acctgtcgct
    19321 gaagtattat ccgcaggtct cgaacggcgc cttcgacaac ccgcgcgccg aggtcgtcat
    19381 cgccgatggc ctgaagtatg tggccgagac cacggagaaa ttcgacgcca tcatcgtcga
    19441 tagctcagag ccgatcgggc cctcggcggt gctccacacg gaggagttct tcgcggactg
    19501 caggcgtgca ctgaagccgc acgggatcct ggtgacgcag aacgggctgc ccttcctctg
    19561 ccccgaggcg ttctcgaata ccacccggat cctctcgggc ctgttcaacc gctgtgcgcc
    19621 ttatctctgc catcagccgt gctatttcgg cggcgagttc gcgatcaact gggcctctga
    19681 agagccggcg catctggaga tttcggatgc aaagctcgcg cgccggcaga aggcgcgggg
    19741 gatagagacc cgctactgga cgcccaagtt ccacgtcgca gcctttgcat tgcccgccta
    19801 tgcggaaaag ctggtgaacg aggcaagagc cgcggcgagg ggcaaggcca ccaaggcaca
    19861 gcctaaggtc agcaagtcca aatgaccggc ttcgtagccg gtcgccagcg gcgcggagcc
    19921 gggccgccaa cgcgcgttgg cggccaaagc ctcagtgctt gtgccccgcc tcgctgtgat
    19981 gatggtctcc agctaccgcg ccgctggggc ctttggcgcc gatcggctcg accgacgccg
    20041 tgacgggaat ttcacccgct ttctcgaact cgagcgtgag ctgcaccttc tctcccgcct
    20101 tgagcggatg cttgaggtcg aagagcatca ggtgatagcc gcccggcgcg agtgtcaccg
    20161 tctccccggc agggatctcg atcgcatcga cccggcgcat cttcatgacg ccgttctcat
    20221 ggacgtgatt gtgcaactcg acacgttcgg ccacatcgct cttagcagcc accagccgat
    20281 cgcctggccc cgtgtccgag atcgtgagat atgccgcgcc ggtttttacg ccgccgggtg
    20341 tcgcccgcaa ccaagcctcg gagacagcga tgccatcgga ggaaccctgc gcaaaagcaa
    20401 gcgaacccat gagcggcagc aagatgcccg caatcaaaac tctcatgact gtcattgaaa
    20461 tttagtcctc ccgctgaatc gatgagccac cggaccgcac gttgcggtcc accgaaggtt
    20521 tggccctcga tgtctggacc acacccggcg gatgcagcgc agcgatcagg acgtaacagc
    20581 ccggcactcc tcgtcgggag gtaggggatc gcagtccttg ctgacaacgc acgcctgcat
    20641 cgcacgccgc aggtcggcag tataggcgcc gtcgatcggg ccaccgtaga gacctagtgc
    20701 cgcaagcccg cactggtaga gctggatcat ccgttcgccg ccgaaccgca tggccgcatc
    20761 atagtcgcgc agcgcgccgg ccgggtcacc ggttacctga cgaatgtgtg cccgcgtgtc
    20821 atacgcatgg gggctggtcg gctgcagctg caatgagcgg tcgacgtcgg gccggccggc
    20881 ttccggccgc cccgatctga aatacgccca ggcccggttg ttgagcgcga cggcaaaatc
    20941 cggcaccagc cggatcgcct cgtcgtagtc gcgaatggcg atgtcgtaga ggcctttcag
    21001 cgagtagcca agggctcgca tggcgtacac cgagccaagc atctggcctg tcaagcccgc
    21061 gccctcgatc agctccgtgc aacctttgat gcgccgctcg ttgtccgacg agaagcagtc
    21121 atggccatca agtggatctg cggcagccag gggcgctgcc gcgaccagga gcgcaatcgc
    21181 cactgcccat cgtttcatcg tcctcgatcc tcgcacggcc tcattcgctg gtcccggatc
    21241 gtcctccgac cggaaactga cgattcgatg gcattctcaa agatgtgttg tggaatcgcc
    21301 gagttccagc gaaggtttgg caatatttgc tgggaatcaa ggccaggcgc gccatcgcca
    21361 gctagcatcc aacgagagcg tagaaagaaa gcggttgcct caaatcggaa cttggccggc
    21421 cgaaggcacg tttcgttcta cgccaaagtg caagcagatc agacggctgg aggggccgat
    21481 cagcctctag ccagtcggcg gagagtggaa ccattatgaa gcgagccgac ctcattgcgc
    21541 tagcgcaatc gaccggtttt cccaaagtca gcatctatct cccaactcac ctgagttatc
    21601 cagaaatcga gcaggacccg attcgccttt cgaccctgct caaggaggcg gaccaaaagc
    21661 tcgccgcgtg cggcgttcgc cggccggaaa tcgacgatct cctcgcagag gcgcgagggc
    21721 gcgttcctgc ggccatgttc tggcgctacc aggaccacgg cctggcggtg ctgatcgaga
    21781 aaggcacgac gcggttcgtg aagttgcctc gggaggttcc cgagctcgtc gtcgtcgggc
    21841 cccgctacca cctgcggccg atgatccgca tgttccggaa tggcgagaac ttcaatctgc
    21901 tggccgtcac gcgggaaaag gtcgccctct acgacggccg ggaacgcgag ctgcgcgaag
    21961 tgacggtcga gggcatgccg accagctttg ccgacgtcgc gcgtcgcacc gaatttgaga
    22021 cgaccttcaa ctatgcgtcc cgcgaccgcg gcgacccccg gagcggccac gagtccggca
    22081 agttccattc gctcggcgtg agccctgaag actacgagtc gatcgagctc gatcgttttc
    22141 tcctcgatgt cgccaaggcc gtggaccgcc acctcgccaa cagcgaggcc ccgctggtgc
    22201 tcgcggccca gccccgcatc gtcgggcatc tccgcaagca tctcgactat cgttatgtca
    22261 cacaggaaga catccagtcg gacccgtttg cgttgacgga gcaggagttg cacgctgaag
    22321 gctggcgcat cgcggagcct gtggtccgtg ccgatcgcga gaatgctcgc cgcagattgc
    22381 gtgcatacct ggagggtgca ggagttccgg gcaacaagga tctgcagacc ttgatgagag
    22441 aggccattgc cgggcgtgtg gagtgcgtgt tccttgcacc ggatgcgaat gtctggggcg
    22501 tcttcgatga ggaataccag gtgctccgca tcgatccgga gcctgggccc gaaaatgagg
    22561 atctgctgaa ccgcctcgcg gtcgagacgc tcatccgcag tggggacgtg ttcacccttc
    22621 ccgaagatct tcgctaccgc gccggccccg tcgcgggcct ctaccgctat taggcgaagt
    22681 ctgtgccttt caggtcgacc cgggcgattg cgcccgggat cgatcgagcg agccgcgact
    22741 actaagagct ggccgcaact aagaacgacg gccgccgtac aggacggcgg ccgtttctcg
    22801 tcgtcccatc aattcaagtg ttcaggtcgc ttgcgggttg aaccgcatcg ggcggatccg
    22861 gcgcaagctg gtggcctgcg gaccgcgtgg actgtcgtcg gaattgatcg tcagctcgac
    22921 cgggtcgccg acctccaggt cctcgaacgc gtcgtcgatc acagcattcc tgtgaaagta
    22981 gacccgacgg ccatcggtga cttcgacgaa tccatagccc tcgtcgtaga agatgatgtc
    23041 gatctgcccc tgcggcttgc cctcgtggac ctttacctcg ccgtactgct ggcggcggcg
    23101 cttcttaagc cggcgttcga gagcggcgaa gctgtcgcga accgcgacca gagcgtcgga
    23161 gtgggcattg atgtcgcccg gcttgcgccc gacggcgatc tctgctcccg gcacgcgcgc
    23221 ctcgatcgtg accttgaact ggcctgtgcc cggaactggc ccattgacgt ggacgtgcca
    23281 cgtggtgatg tcgttatagc gacgttgcag cttgtttacg tactcgtgga ccttgctcct
    23341 gatggtcacc gacggctcca tgccgtggaa cgacagcttg agattcgctt ccgtttctgg
    23401 gtttttcatt tcacccacct gatcattccg gtgctgcctt gaacgccggc tcagtgccgc
    23461 agttccttgc cggctccaca ctcaacctct tggacgtttg cggctatgcg caaaatcaat
    23521 cgtcgcagta gggataaatg gtgtcgatag aggtcgaaag cgagaggccg gctgcctgct
    23581 gccgaagagg acctccaggc cgggcgctgc gttctccttc tgccttggtt ccgtccggag
    23641 caagtcgatg acaggtgagg gaaaagtcgt gatcgtaacc ggcgctgccc gcggcatcgg
    23701 ggcagcgacg gctgtccgcc tggcccgaga ggggtggaag gtggtgctgg ccgacatcga
    23761 tggggccgcg ctcgccaacg tggcaaagcc ggccaacggc tatgctgtcg tttgcgatgt
    23821 cggcgtcgaa gccgacgttc agcggctggt ggacgagagt gtcgaccagt tcggccggat
    23881 cgatgcgatc gtctcgaacg ccggcataaa cctgcgcggc ccactttccg agctcacgct
    23941 cgacagctgg aaccgcgtcc tcgcggtgaa tctcacggct gctttcctgc tggcccgtgc
    24001 agccgagcgc ccgctgcgcg acgcgaaggg tgccatggtg ctgattgcgt ccacacgggc
    24061 gcacatgtcc gagccggata cgctgcccta ttcggcgagc aaaggcgggc tcgtggcctt
    24121 gacccacgcc ttggcaatgg ccttggcgcc ggttcgggtc aattgcattt ctcccggctg
    24181 gatcgacacc ggcaagtcgg gcccgctgtc gccggaggac cacgctcagc acccggtcgg
    24241 ccgcgtcggg cagaccgacg acatcgcggc cgcggtgtct tacctgctct ccgacggggc
    24301 tggcttcatc accggcgccg agctgatcat cgacggcggc atgacccgca agatgatcta
    24361 cgtctgatct tcagcctgcg atgatgccgg cgccacggaa gccgccatcg acggcaacga
    24421 cttcgccggt cagatagctc gcctggcggt catcgagcag aaagcagatc gcgggcgcga
    24481 tttcctccgg acggccgtag cggcgcatgg ggatgaagcg tgtccacgtt tcgcggtcct
    24541 cggcggtgtg caggtcacgg acgagcggcg tatcgatcgg ccctggcgcc acggcgttga
    24601 cacggatgcc gtggcgcgcg aggtcgtttg ccataacctg cgtcagcaca atgaccgcgc
    24661 ctttggatgc gccgtaggcc gaccgcccct tcgagcccct cacgcctgcc ac
//
LOCUS       DASVDU010000199         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_263342, whole genome shotgun sequence.
ACCESSION   DASVDU010000199 DASVDU010000000
VERSION     DASVDU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263342"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /gene="prs"
                     /locus_tag="VF226_12840"
     CDS             complement(<1..770)
                     /gene="prs"
                     /locus_tag="VF226_12840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX7074920.1"
                     /translation="MLIFALSDSAAFGERIAQGLGLALSQLEERIFPDGEFQARPLVN
                     LRGRDVYVIQSLHAGPETSIGGKLSRLLLFLATARENGARRVTAVMPYLAFARQDRRV
                     HPHDPLALRSLALMLEAVGVSAVVTVDVHNIAAFENAFRCCTVNLEAGPILARKAMEI
                     GLDDPLVVASPDLGAAKRAQAFGSVLKQARGGPVGLAYMEKQRLDSGALAGTLFAGDV
                     EGAHVLIVDDMISTGTTLVRAAKACRDRGARRIHAFAT"
     gene            1059..1559
                     /locus_tag="VF226_12845"
     CDS             1059..1559
                     /locus_tag="VF226_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7074921.1"
                     /translation="MAKSELTKPTPSQLSRPSDIFSVMRDEMDRMFERFERGWPRLPG
                     LISRGLGREGVMLPEVDVYEDQNKVTVEVDLPGVDEKDVSVTMADGVLTIKGEKKSER
                     EEKEENYYLSERSYGAFERSLTLPDTIDENKIEAQFDKGVLKIIAQKRPEAVKPEKKI
                     EIKKVS"
     gene            1695..3053
                     /locus_tag="VF226_12850"
     CDS             1695..3053
                     /locus_tag="VF226_12850"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX7074922.1"
                     /translation="MDLSKTPLLTDLYQLNMLQAYLDHGMTEIAVFEFFVRKLPPSRG
                     FLVAAGLEQALDFLENLSFQPEDIAWLRSSRRFSENLLSELETLRFTGDVYAMPEGSV
                     AFPNEPLLRVVAPLPLAQLVETRIINILHFQTLIASKAARHVLLAPGKRLVDFGLRRA
                     HGAEAGLMAARASYIAGYAGTATMSAGQHYGIPIFGTMAHSFIQAFDDEVAAFEAFAE
                     SRPEGLVLLIDTYDTEEAARKVVSLARKLAARGIRVSGVRLDSGDLVALSKSVRRILD
                     DGGLRDVTIFASGGIDEKELIEFRDANAPIDGYGIGTSLTTSFDVPALDCAYKLQEYA
                     GIGRRKRSAGKATWPGRKQVWRSYGTDGRMARDVLSLENDSQPGDPLIQLVMKNGRRI
                     GPRPTLDEIRERAANDLARLPEPLHRLEPGAGYPVEIGEALLRYTEEVDRHLEATESR
                     SA"
     gene            3050..3703
                     /gene="pncA"
                     /locus_tag="VF226_12855"
     CDS             3050..3703
                     /gene="pncA"
                     /locus_tag="VF226_12855"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX7074923.1"
                     /translation="MTDKFQTTDSDVLIVVDLQYDFCPGGRLAVPRGDEVIGPINLLA
                     RRFKHVLLTQDWHPPGHLSFASSHPGRQPYETITVSYGPQVLWPDHCVQGTRGAELRD
                     ELDIPHAELILRKGYHRDIDSYSVFFENDRTTPTGLAGYLRERGLSRAFFAGLALDFC
                     VRYSVEDAHRISLDAVIVEDACRGIGVNGSLESTRQSISQLGIPLVTMEDISAGGAA"
     gene            complement(3707..>4019)
                     /locus_tag="VF226_12860"
     CDS             complement(3707..>4019)
                     /locus_tag="VF226_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HEX7074924.1"
                     /translation="EIWHGKLVLYGCGDLINDYEGISGYEEFRDDLSLIYFPKVKAKD
                     GEGALESLEMAPFHMRAFRLEKADAQDSAWLADLLTEQGRQFGTWAELASDGNLRLGW
                     S"
BASE COUNT          799 a         1242 c         1232 g          746 t
ORIGIN      
        1 tgcgttgcaa aggcgtgaat gcggcgtgca ccacggtcac ggcacgcctt agcggcgcga
       61 acgagtgtcg tgcccgtact gatcatgtca tcgacgatca ggacgtgggc gccctccacg
      121 tcacccgcga atagagtgcc cgccagtgct ccgctgtcga gacgctgctt ttccatgtag
      181 gcgaggccga cggggccgcc acgggcctgc ttcaggacgg agccgaacgc ctgagcgcgt
      241 ttggccgcgc ccaggtcggg agaagcgacg acgagggggt catccaggcc gatctccatc
      301 gctttgcggg ccaagattgg gccggcttcg agattgaccg tgcaacagcg gaaggcattc
      361 tcgaatgctg caatgttgtg aacatcaacc gtcaccacag cactcacgcc gaccgcctcg
      421 agcatcagag ccaggctgcg cagagcaagc ggatcatgcg ggtggacccg cctgtcctgc
      481 cgggcgaatg ccaggtaagg catgaccgcc gtcacccgcc gcgccccatt ctctcgcgcc
      541 gtcgcgagaa acaggagaag gcggctcagc ttgcctccaa tgctcgtttc gggcccggca
      601 tggaggctct ggataacgta aacgtcccgt ccgcggagat tgacgagtgg gcgtgcttga
      661 aattccccat cggggaaaat ccgctcttcg agctgcgaca atgccagacc gagcccttgg
      721 gcaatccttt ctccaaaagc tgcgctgtcg ctcaaggcaa agatcagcat accggaaacg
      781 ttctcctgca aatgaactgt ggctgcctca ggcaatctta actcttcgac tgctcgatga
      841 gctgcgggcg gcaaccattt tgtcccgttt gcgtcatcag gcaggcaaat gcccggtcag
      901 cttgatgccg atcaatgaga agcttccgcg accgtggcaa tcagcaaaca tccttgctag
      961 ccggctggtc agcgcccccc ggccgcgggg gcgcaccagg tgaaccggca cccgcacttc
     1021 gcgttttcga atactacctt cgaacaatgg agggccacat ggccaagtct gagcttacca
     1081 agccaacccc gtcgcaattg agccgcccaa gcgatatctt cagcgtcatg cgcgacgaga
     1141 tggaccgcat gttcgagcgt ttcgagcggg gctggccgcg gctgccgggt ctcatctctc
     1201 gtggtctggg acgcgaaggc gtcatgcttc ccgaggtcga cgtttacgag gaccagaaca
     1261 aggtgaccgt cgaagtcgat ttgccgggcg tcgatgagaa agacgtttcg gtaacgatgg
     1321 cggacggcgt gctgacgatc aagggcgaga agaagagcga gcgcgaggaa aaggaagaga
     1381 actactatct ttccgagcgc agctacggcg cgttcgagcg ctcgctgacg ctgcccgaca
     1441 ccatcgacga gaacaagatc gaggcgcagt tcgacaaggg cgtcctcaag atcatcgccc
     1501 agaagcggcc cgaagcggtg aagcctgaga agaaaatcga gataaagaaa gtatcttgag
     1561 cggcatcact gcctagcgag ccgcgtcgac ctgctccggc cttttgaaat cggccagagc
     1621 gggatatgat agacgcaatc actggcgcgc tgcgtatcga ccgcaacgcg ccagtattgt
     1681 gatgagattc ggggatggac ctctcgaaga cgcctctgct caccgatctt tatcaattga
     1741 acatgctcca ggcctacctg gatcatggaa tgacggagat tgccgtcttc gagttcttcg
     1801 tacgcaagct cccgccatcg cgcggctttc tggtcgcggc gggattggag caggcgctcg
     1861 actttcttga aaacttaagc tttcagccag aggacatcgc gtggctgcgc agcagcaggc
     1921 ggttcagcga gaacctgctc tccgagcttg agaccctgcg cttcaccggc gatgtctacg
     1981 ccatgccgga aggcagcgtg gcctttccca acgaaccact tttgagggtc gttgcgccat
     2041 tgcccctggc gcagctcgtc gaaacacgca tcatcaacat cttgcatttc cagacgctga
     2101 tcgcctcaaa ggcggcgcgt cacgtgctgc tcgcccccgg caagaggctc gtcgatttcg
     2161 ggctgcgccg ggcgcatggg gctgaagccg gattgatggc cgcgcgcgcc agctacatcg
     2221 cgggctatgc cgggacggcc acgatgtcgg ccggccagca ctacggcatt ccgatctttg
     2281 gcacgatggc ccattcgttc atccaggcgt tcgatgacga ggtggcggcc ttcgaggcct
     2341 ttgccgagtc gcgccccgag ggcctggtgc ttctcatcga cacctacgac acggaagaag
     2401 cggcacggaa ggtcgtctcg ctcgcgcgca agctcgcggc gcggggcatc agggtcagcg
     2461 gagtccggct cgacagcggc gacctcgtcg cgctctcgaa gagcgtgcgt cgcattctcg
     2521 acgacggggg cctccgggac gtgacgatct tcgccagcgg cggcatcgat gaaaaagagc
     2581 tgatcgagtt tcgtgacgca aatgcaccga tcgacggcta tggcattggg accagtctga
     2641 cgacgtcgtt cgatgtcccg gccctggact gcgcttacaa gctccaggaa tatgccggga
     2701 ttgggcgtcg gaaacggtcg gccggcaagg caacctggcc cggacgcaag caggtctggc
     2761 gcagctacgg gacagacggg cgcatggccc gagatgtcct ttccctggaa aatgacagtc
     2821 agcctggcga tccgctcatt cagctggtca tgaaaaacgg gcggcggata ggccctcggc
     2881 cgacgctgga cgaaatccgc gaaagggctg ccaatgacct cgcgcgcctg ccggagccgt
     2941 tgcaccggct cgagcccggt gccggctacc cggtagagat tggcgaggcg ctgctgcgat
     3001 atacggagga ggtcgaccgg cacctcgaag cgacagaatc gagaagcgca tgactgacaa
     3061 gtttcaaacg acggacagcg acgtcctcat tgtcgtggac ctgcaatacg acttctgccc
     3121 gggaggtcgt ctggcggtgc cccgcggcga cgaggtcatc gggccaatca atctgcttgc
     3181 ccgccgcttc aaacatgtcc tcctgacaca ggactggcat ccgccagggc acctttcgtt
     3241 cgcttcctcg catcccggcc ggcaacccta tgaaaccata acggtgtcct atggccccca
     3301 ggtcttgtgg ccggatcact gtgttcaggg cacgcgcggg gccgagctgc gcgacgaact
     3361 cgatattccg catgccgagc tgattctgag gaagggctat catcgagaca tcgactccta
     3421 ttccgttttc ttcgaaaacg accggacgac gccaacgggg ctcgccggct atctgcggga
     3481 gcggggcctc tcacgcgcgt tcttcgccgg cctggcgctc gacttctgcg tccggtactc
     3541 agtcgaggac gcccatcgca tcagccttga cgccgtgatc gtcgaagacg cctgccgagg
     3601 catcggggtc aatggctcat tggagagcac gcgccagagc atttcccagc tcggcattcc
     3661 actcgtcacg atggaggata tttcggccgg cggagcggcc tgaggctcag ctccatccga
     3721 gccgcaaatt gccgtcggac gctaattccg cccatgtccc gaactggcgt ccctgttctg
     3781 tcagaagatc ggcaagccag gcggagtcct gggcatcagc tttctccagg cgaaacgcgc
     3841 gcatgtgaaa tggtgccatt tccagggact ccagcgcgcc ttcgccgtcc ttcgctttca
     3901 cttttggaaa atatatcagg ctcagatcat cgcggaactc ctcgtatccg ctgatgccct
     3961 cgtagtcatt gataaggtcg ccacaaccgt agagaacgag cttgccgtgc cagatttcg
//
LOCUS       DASVDU010000200        19353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1603960, whole genome shotgun sequence.
ACCESSION   DASVDU010000200 DASVDU010000000
VERSION     DASVDU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19353
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603960"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..962)
                     /gene="proC"
                     /locus_tag="VF226_12865"
     CDS             complement(138..962)
                     /gene="proC"
                     /locus_tag="VF226_12865"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX7074925.1"
                     /translation="MTLSLNGKLLLVGAGKMGGAMLAGWLERGLDPAQIIVQDPAPPP
                     ESAELVSKHGIERREQGAVLPEAPAVILVAVKPQIMDQVFPEVATQAGPETVVLSIAA
                     GRTIASFEAHLPAGTAVVRSIPNTPAAIGRGITVCCANGHVSAAQREICQSLLTAIGE
                     VGWVEDESLIDAATAVSGSGPAYVFLLAECLAEAGRTIGLDPDLAKRLANATVSGAGE
                     LLRRSGQEPATLRKNVTSPGGTTAAALSVLMADDGLQPLLTRAVEAAFKRSRELSG"
     gene            complement(1096..1593)
                     /locus_tag="VF226_12870"
     CDS             complement(1096..1593)
                     /locus_tag="VF226_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX7074926.1"
                     /translation="MASAEAGYEHMSNPIDIVEQVAHAHDWACDRTADDEVTLIVAGS
                     WADYHVSLNWRDDLEALHLACAFDFKVPENRLPEIYRLVAQINEQLWLGHFDLWTQEG
                     LIMFRHGLMLNGALATPRQCEALLKAALEACERYYQAFQFVVWAGKESREALVSTMFH
                     TEGQA"
     gene            complement(1909..2175)
                     /locus_tag="VF226_12875"
     CDS             complement(1909..2175)
                     /locus_tag="VF226_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX7074927.1"
                     /translation="MTQTTNRFFDELAKLMTDAAGAAQGLRREFETLMKSQGERILRE
                     MDVVRRDEFEAVKAMAQRAREENDALTARVAALEAELAQLKSKP"
     gene            2341..3195
                     /gene="lgt"
                     /locus_tag="VF226_12880"
     CDS             2341..3195
                     /gene="lgt"
                     /locus_tag="VF226_12880"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7074928.1"
                     /translation="MSLLASFLAIPFPNIDPVAIQLGPIAIKWYGLAYMAGLLLGWLY
                     IRRLISTPALWRAGVPPFSLEKTDDLLFFMTLGVVLGGRLGFVLLYEPAYYLKNPLEI
                     VQVWRGGMSFHGALVGCGIAIVMFARRNKLSPWSAMDLCAAAVPIGLFFGRVANFING
                     ELFGRPTSVPWGMVFPEAMYQFAAIEPTPRHPSQLYEAFFEGLVLFLVLRWMTHRMGA
                     LKNPGLVSGAFLIGYGLARSFSELFRQPDPLHALTVGILTPGIAYSLPMIVLGLYFIR
                     SARSQLAP"
     gene            3211..4335
                     /locus_tag="VF226_12885"
     CDS             3211..4335
                     /locus_tag="VF226_12885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX7074929.1"
                     /translation="MTAAHEPTPLALKLKEQIRREGPLPVYAYMRACLGDPEHGYYRR
                     RPAIGVAGDFTTAPEISQTFGELIGIWAAVAWRAMGAPPEVDLIELGPGRGTMMSDAL
                     RAARVVPGFLEAARVQLVESNPLLREVQRETLTAGAQQVPISWHEAFTADVAGGRPAI
                     VLANEFLDALPVRQLVFVENQWRERCVGLDAEDEFLLVPGELVELERSPSSEPAQEGD
                     VLEMRPDLVGISTILAQWAAQPLAALFIDYGHTETAFGDTLQAVSGHRYASIFERPGE
                     TDLTAHVDFAELRRLCIERGLRFDGPITQSEFLLGLGLAERATKLMAAARSDEVGLIE
                     AGAHRIADPLGMGGRFKAACVRSANVPVLPPFPAAGAKGG"
     gene            4340..5122
                     /gene="pgeF"
                     /locus_tag="VF226_12890"
     CDS             4340..5122
                     /gene="pgeF"
                     /locus_tag="VF226_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX7074930.1"
                     /translation="MAVDGEIIRAECLSDAAGISHGFFTRQGGVSTGIYGALNCGLGS
                     KDDPALVRENRARVARALGAPREEVTTLYQVHSATALVIDAPFPPGAVPRADALVTRT
                     PGLVIGAMAADCTPVLFADPGARIVAAAHAGWRGALGGILESTIAAMVGLGADRSRIR
                     AAVGPCINQSAYEVGPEFEAEFLRADPENARFFARNSTGTRSHFDLPGYVEHRLSRAG
                     LAIVERRSPCTYENESRFFSYRRATHRKEPDYGRQISAIVLT"
     gene            5323..6351
                     /locus_tag="VF226_12895"
     CDS             5323..6351
                     /locus_tag="VF226_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074931.1"
                     /translation="MTTIHAAAASHLVRRLAFAALATVFAAGLGACSSDTNILGNAGD
                     GSQLLSQQTVQPAPTSSQIAIAPVIGAPESVGKQIVAQLTQDLEQKKVAVAKAPTDRA
                     DFTLRGYIVAAKEKNGTKVSYIWDVTDPTGQRVNRITGEEVAGKTDSRDPWAAVTPEV
                     MHSITSKTATSLGTWIQSATAVAGSGQTAQPVQTAALGSSTAAQASSAPVAPPAATAS
                     ISNSGTVTAVVPSVTGAPGDGSVSLTRALQRELSRNGVALAEGPAGNAYRVEGRVKMG
                     ASQNGKQPIQIDWYVKDPQGKQLGTVSQKNEIPAGSLDGEWGKVADAAASAAAQGILK
                     LLPGKGVN"
     gene            6462..7436
                     /locus_tag="VF226_12900"
     CDS             6462..7436
                     /locus_tag="VF226_12900"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7074932.1"
                     /translation="MNDAGRKNHFDGLKIKLVAGNSNRPLTEEISSVLGVPLTKGVCR
                     RFADMEIFVEIQENVRGEDVYVIQSTSFPANDNLMELLILIDALKRASARRITAVIPY
                     YGYARQDRKPGPRTPISAKLVANLIERAGANRVMTLDLHAGQIQGFFDIPTDNLFAAP
                     VMVRDIKERFELDNLMVVSPDVGGVVRARALAKRIGAPIAICDKRRERPGESEVMNVI
                     GNVRGKRCILVDDIVDSGGTLCNAAEALLQNGAIEVMAYITHGVLSGGAVARIQNSSL
                     KSLVITNSIDPTEAVRASKNIRILSIAPLIAEAIARTSREESVSSLFY"
     gene            7620..8240
                     /locus_tag="VF226_12905"
     CDS             7620..8240
                     /locus_tag="VF226_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX7074933.1"
                     /translation="MAEVVEIPATARPRAGKGAARAVRRQGKIPAVIYGEKQEPETIA
                     LDANELWKVVQKGRFLSTVLDINVDGQKRRVLPREVQLDPVKDLPLHVDFQRVGASDY
                     VRVVVPVRFINEGASPGLKRGGVLNVVRHEVEVTCPPDRIPEALIVDLTGLEIGRSIH
                     ISAVPLGEGVRPTITNRDFTVATIAGKKTDEDRAAEAAEGAEAEKK"
     gene            complement(8346..9788)
                     /locus_tag="VF226_12910"
     CDS             complement(8346..9788)
                     /locus_tag="VF226_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7074934.1"
                     /translation="MTEPKRPELAAPTPELIAKLTEIVGPEHALTDPDQQLPYLREWR
                     DLFIGRSPVVLRPGTTEEVSRILALANEARVAVVPQAGNTGLVGGQTPFETGTEIVLS
                     VSRLKRVREINPAAYHMTVEAGLTLAECHAAAERVDRLFPLSLPSEGTCQIGGNLATN
                     AGGVGVLAYGNARKLVLGLEVVLADGRIVPALKALKKDNTGYDLKDLFIGSEGTLGVI
                     TAAVLKLFPRPAESATAMVALAKISHALDLFSLAQETAGRSVTAFEFIPRICIDFVLR
                     NVPGTREPFPQKYPWYVLIEISGAKPDGQAARDMENVLGQATERGLLLDAVLASSLSQ
                     ARELWRLRESVSESQKPEGGNIKHDVSVPIARIAEFIERADRIVESICPGARPLPLGH
                     FGDGNIHYNIAQPIGMEKERFLELWEPISSAVHALVAEMDGSISAEHGIGRMKRADLV
                     RYKSPVEIDLMRRIKAAFDPNGILNPGKVL"
     gene            9979..10542
                     /gene="pgsA"
                     /locus_tag="VF226_12915"
     CDS             9979..10542
                     /gene="pgsA"
                     /locus_tag="VF226_12915"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEX7074935.1"
                     /translation="MSLPNILTYGRIIAVPGLVACLFFLHGDLARWSALVLFVTASLT
                     DWLDGYLARAWQQQSALGRMLDPIADKLLVGTTLLMLTHFNTIYGWHVWAAVIILCRE
                     ILVSGLREYLAELNVKIHVTQLAKWKTTLQMIALGVLLAGPAGDKLLPVVTASGLALL
                     WLAALLTLWTGYDYLRAAVQHAIGGSE"
     gene            10554..10835
                     /gene="moaD"
                     /locus_tag="VF226_12920"
     CDS             10554..10835
                     /gene="moaD"
                     /locus_tag="VF226_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEX7074936.1"
                     /translation="MAEQNTGGTTVKLLYFAWVREKVGRAEEAIELPAGIETVGELVG
                     WMKSRGPEYAAAFERSGVIRAAIDQKHVPPSASIRGAREIAFFPPVTGG"
     gene            10839..11294
                     /gene="moaE"
                     /locus_tag="VF226_12925"
     CDS             10839..11294
                     /gene="moaE"
                     /locus_tag="VF226_12925"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HEX7074937.1"
                     /translation="MAVRVQREDFDVGAEISRLTTGRSDIGAVVTFTGLVRGNSTGRP
                     VAAMTLEHYPGMTEAELARVEAEAQARWPLQASLIIHRIGELKPGDNIVLVVTASAHR
                     HAAFEAAEFLMDYLKTRAPFWKKEADPDGRGSWVDARESDEAAAERWHS"
     gene            complement(11597..12082)
                     /locus_tag="VF226_12930"
     CDS             complement(11597..12082)
                     /locus_tag="VF226_12930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7074938.1"
                     /translation="MDGALYRAIMRHQAGAVTVIAVGPPGRRSGLTATAVCSLSDDPP
                     TILACIRRTARTHDVIHRLGVFSVNLLASDQQAIAELFSGGMGLSGEDRFKEDDWTTL
                     ATGAPILRGALASLDCRLEEQHGFATHSIFIGSVEDGTVRAEAEPLLYFRGDYWDLGG
                     R"
     gene            complement(12133..12855)
                     /gene="pth"
                     /locus_tag="VF226_12935"
     CDS             complement(12133..12855)
                     /gene="pth"
                     /locus_tag="VF226_12935"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX7074939.1"
                     /translation="MKLFVGLGNPGDKYARHRHNIGFMAVDRIAERHRFPPWRRRFQG
                     ETAEGTIGGERVLLLKPMTYMNESGRAVGEAARFFKIDLQDVVVFHDELDLAPGRVKV
                     KVGGGNAGHNGLRSISAHLGNDFVRVRIGIGHPGSKDAVIPYVLHDFAKADQEWLDPL
                     LDAIANASGRLASGDTQRFLTDVARAMQEATREAPSPEEAKAKPPATQPRPLHPAGER
                     MGKRQSALAENLKRWLAGRSKP"
     gene            13008..13457
                     /locus_tag="VF226_12940"
     CDS             13008..13457
                     /locus_tag="VF226_12940"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7074940.1"
                     /translation="MTATGKTRVTEPCWPKAAASAAIFRGNAVLLVERGKPPVSGVWS
                     LPGGHIEPGEPARDAALREVTEETGIRAELLGLADVHDVIIRDEAGALQAHFLLAVFY
                     GRWLAGEPAAATDCRDARFVPLDEIGTYPLTDGAQRIIQKAARLLRG"
     gene            complement(13493..14572)
                     /locus_tag="VF226_12945"
     CDS             complement(13493..14572)
                     /locus_tag="VF226_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7074941.1"
                     /translation="MSWSFPLFRLGGTLVQVHWTFFLLLAWFGAIQWMAVGPEAAVFA
                     IIFIILLFLCVLLHEFGHIFAARRYGIKTPTITLLPIGGVASMERMPEKPSQEVIVAL
                     AGPAVNVVIAFVLIAILGARIDPSALDLEQNPGTNMMARVAVANVALVVFNLIPAFPM
                     DGGRVLRALLAMKMGFVRATRTAASIGQMFAIFLGFVGLFGNPLLILIAIFVYFAATA
                     ESQYVEMRSLARGFLARDAMITKFEALGPTSTADDAASLLLRTTQQEFPVLDGNSRLQ
                     GIVTRDVLIEALRDRGGSTLVTEFMHKDIPVVSGHSTLDNVVTMMQQKSARAVAVTGP
                     DERLLGFITPENFSEMLMIGRAREA"
     gene            14955..15656
                     /locus_tag="VF226_12950"
     CDS             14955..15656
                     /locus_tag="VF226_12950"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtnX-like HAD-IB family phosphatase"
                     /protein_id="HEX7074942.1"
                     /translation="MLLSRDAIRHVPALPIDVFVDFDGTIAPDDPTDSLFARFADPAW
                     LDIEAEWQSGRVSSREAMARQVGLIRATPSEIARLITQMKIDPGFPEFVALCRRHGAR
                     ITVVSDGLDLLVESMLKAAGLDLPFYANQLVWQGGDRWALRFPYIRSDCRMRMGNCKC
                     AHARFMGIGANVMVGDGRSDFCIAERCDLVLAKGKLAPHCRENGVQHIPIRNFSEATS
                     AMLDWLTGIRSGLTA"
     gene            complement(15659..17548)
                     /locus_tag="VF226_12955"
     CDS             complement(15659..17548)
                     /locus_tag="VF226_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7074943.1"
                     /translation="MQTAMATVCLSGALDEKLQAIAAAGFKGVEIFENDLLSFSGTTA
                     DVRQMVSDLGLSTVTFQPFRDFEGMPEPQRARNFARAERKFDVMEQLGCDLLLVCSNV
                     SPESIGGIDRAAADLRELGERAARRGIRIGFEALAWGRHINDYRDAWEVVRRTDHPAV
                     GLILDSFHILARGTDLKPMRSIPRDRIFLVQVADAPQLEMDYLSWSRHLRCLPGQGEL
                     PLMDFMEALHATDFDGLISLEIFNDQFRAGSARMVATDGQRSLLYLLDQLRQHTGAPI
                     AGLPELPPRSTCHGIAFIEFAVDEVTAPAISRLFGQLGFVKAGIHKSKSVVRWRQGDI
                     NLVVNSEKEGFAHSFNITHGTSVCAIGLDVEDAGRTLERAKKLLARPFRQAVGAGELE
                     IPAARGLGGSLIYFLDRRKPLADVWEIEFTPPAEPVNSRDAGLLAVDHISQSMHYEEM
                     LSWLLFYTSLLDVTKTPGQDVIDPGGIVRSQVVETADGALRIVLNGSQSNRTLSSRFI
                     EEFFGSGVQHIAFSCADIYATAEKLKENGVELLPIPENYYDDLEARWDLTADELARLK
                     ASNILYDRDGSAEYFQIYTPSFQERFFFEIVERRRYVGFGAVNAPIRLAAQARLAKSL
                     GMPDS"
     gene            17883..18122
                     /locus_tag="VF226_12960"
     CDS             17883..18122
                     /locus_tag="VF226_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074944.1"
                     /translation="MSLAKQVFALSLSLAALGYTVIPAVQAAVKPGPGPLDSQSMIVQ
                     VQKKAKGKATCGTYMYWSAKERKCVDARLKKSKGS"
     gene            18263..19353
                     /locus_tag="VF226_12965"
                     /pseudo
     CDS             18263..19353
                     /locus_tag="VF226_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
BASE COUNT         3436 a         6296 c         6171 g         3450 t
ORIGIN      
        1 ggccgctcgg ctgccagatt catcgccgca gcgattatcc ggccgcgcaa tttcgattga
       61 tccagcatgg cacgcctccg tgtcgatgaa agacataggc gcgaccgggc gcgaatggaa
      121 ggctgcctgt cccggtgtca gccgctcagc tcacggctgc gcttgaaagc ggcttccacc
      181 gcgcgcgtca gcagcggctg caggccatcg tctgccatca gcacgctcag cgccgctgcc
      241 gtcgtgccac ctggcgaggt cacgttcttc cgcagagtgg ccggctcctg acccgagcgc
      301 cgcaacagct cacctgcgcc tgagacggtc gcgttcgcca atcgcttggc caggtccgga
      361 tcgagtccga tcgtgcgtcc ggcctcggca aggcactcgg cgagcaggaa cacgtatgcc
      421 ggcccagagc ccgagacggc agtcgccgcg tcgatgagcg actcgtcctc cacccagccc
      481 acctcgccga tcgcggtgag cagcgactgg cagatctcgc gctgggctgc cgacacgtgc
      541 ccattggcac agcaaacggt tatgcctctc ccaatggcag ccggcgtgtt cggaatggag
      601 cgcacgacgg ccgtgcccgc gggcagatgc gcctcgaaac tggcgatcgt cctcccggcg
      661 gcgatcgaca gcaccaccgt ctcgggcccc gcctgcgtcg ccacctcggg aaagacctgg
      721 tccatgattt gaggcttgac ggcgacaagg atgacagccg gcgcttccgg caggacggcc
      781 ccctgctcac gcctttcgat gccgtgcttg gacacgagct cggcgctttc gggcggagga
      841 gccgggtcct gaacgatgat ctgggccggg tcgagaccgc gctcgagcca tccggccagc
      901 atggccccgc ccatcttgcc ggcgccgacc agcaacagct tgccattcaa gctcagcgtc
      961 attgtctcac ctcgccgcgg caccgcgggg ttgccaagtc gtgaagtcgt caactcgtga
     1021 aatcgtgcag gttctcacga tccagccgaa ctaacgatct cacgatctca cgatttcacg
     1081 aattcaccca cacgctcaag cctgtccctc ggtgtggaac atggtcgaga ccagcgcctc
     1141 gcggctctcc ttccctgccc atacgacgaa ttggaaggcc tgataatagc gctcgcaggc
     1201 ctccagggca gccttcaaca gcgcctcgca ttgccgcggt gtcgcaaggg cgccgttcag
     1261 catcaggccg tggcgaaaca tgatgaggcc ttcctgggtc cacaggtcga agtggccgag
     1321 ccagagctgc tcgttgatct gcgcgaccag gcggtagatt tccggcaggc gattttccgg
     1381 caccttgaag tcgaaggcgc aggcgagatg cagcgcctcg agatcgtcgc gccaattgag
     1441 cgagacgtga tagtcggccc agctgccggc gacgatcagc gtgacctcgt catcggcggt
     1501 gcgatcgcaa gcccaatcgt gggcgtgggc aacctgctcg actatgtcga tcgggttgga
     1561 catgtgctca tagcctgctt ccgccgatgc cattcccagc caccccctta gttggtccgc
     1621 cgtgatagcg aagacctacg cgaacaaacc ccggtccccg cacggctaca gcaagccgct
     1681 tgtccgagct gcggacccgg acgaaccggt tgctgagcgg agcgaccaaa tcagatgcga
     1741 ggcacgagtc gcaagctcac cctcgcattg tccacggcca aagtgatcag tttctggcga
     1801 ggcaagcaaa atcatccagt ctctggatga actgtgagtc agccgttgaa aaacctgggg
     1861 atgtcggccg ccgtgtcagc ccgggcacac gcagaatcaa cgcgcttatc agggcttcga
     1921 cttcagctgc gccagctccg cttcgagcgc cgcgacgcgc gccgtgagcg catcgttctc
     1981 ctctctcgcg cgctgcgcca tcgccttcac ggcctcgaac tcgtcacggc gcacgacatc
     2041 catctcgcgc aggatgcgct cgccttggga tttcatgagc gtttcgaact cgcggcgcag
     2101 gccctgggcg gcaccggcgg cgtccgtcat caatttggcc agctcgtcga aaaagcgatt
     2161 ggtggtctgg gtcatggctc tgccggattg gttctgatcg cagttgcatg tcactatggg
     2221 ttgcgtggcg cacgcgtgca agcatgacag cgcacccggt tgacagcggg ccgcgcatgc
     2281 cgccaacgtc cggcacggca aaggccgccc cctcgggttc ccgtcagacg caggcagtag
     2341 atgagtttgc tcgcgagctt ccttgcaatc ccgttcccga acatcgatcc ggtcgcgatc
     2401 cagctcggtc ccatcgcgat caagtggtac gggctcgcct acatggcagg actgctgctc
     2461 ggctggctct acattcgccg gctcatcagc acgcccgcgc tctggcgcgc cggggtgccg
     2521 ccattcagcc tcgagaagac ggacgacctg ctgttcttca tgacgctcgg cgtcgtgctc
     2581 ggcggccgcc tcggcttcgt cctcctctat gaaccggcat attatctgaa gaacccgctg
     2641 gagatcgtac aggtctggcg cggcggcatg tcgttccacg gcgcgctcgt cggctgcggc
     2701 attgccattg tgatgttcgc gcgccgaaat aagctttccc catggtcggc aatggatctg
     2761 tgcgccgcgg cggtcccgat cggtttgttc ttcggccgcg ttgccaactt catcaatggc
     2821 gagctgttcg gccggccgac atcggtgccc tggggcatgg tgtttcccga ggccatgtat
     2881 cagttcgccg ccatcgagcc cacgccgcgg catccgagcc agctttacga ggcatttttc
     2941 gaggggctgg tgctgttcct cgttctgcgc tggatgacgc accgcatggg ggcactgaaa
     3001 aatcccggcc tggtgtcagg tgcattcctg ataggctacg ggctcgcgcg gtccttctcc
     3061 gagttgttcc ggcagccgga cccgctgcat gcgctgaccg tcggcatcct gacgccggga
     3121 attgcctatt cgctgccgat gatcgttctt gggctttact tcatccgctc ggcgaggtcg
     3181 cagcttgcgc cctgagccga tgaggacctc atgaccgcag ctcacgagcc gacgccgctc
     3241 gccctgaagc tcaaagagca gatccggcgc gaagggccgc tgccggtcta cgcgtacatg
     3301 cgggcctgcc tcggtgaccc ggagcacggg tattatcgcc gccggccggc cataggcgtt
     3361 gcgggcgact tcacgacagc gccggagatc agccagacct tcggcgagct gatcggcatc
     3421 tgggcagcgg tcgcgtggcg ggccatggga gccccgccgg aggtcgatct catcgagctc
     3481 ggcccggggc gcggcacgat gatgagcgac gcgctgagag ccgcacgggt agtgcccggt
     3541 tttctcgaag cggcccgcgt gcagttggtc gagagcaacc ctctgttgcg tgaggttcag
     3601 cgcgagacgc tcaccgcggg ggcacagcag gtgccgatct cctggcacga agcctttacc
     3661 gccgatgttg ctggtggtcg gccggcgatc gttctggcga acgaattcct ggatgccctt
     3721 cccgtccgtc agctcgtctt cgtcgagaac cagtggcgag agcgctgcgt cggtctggat
     3781 gctgaagatg agttcctgct cgtccctgga gagctggtcg agctggagcg ctcgccttca
     3841 agtgagccgg ctcaagaagg cgacgttctg gaaatgcgtc ccgacctcgt cggcatatcc
     3901 acaattctcg cgcaatgggc cgcgcagccg ttggccgctc tcttcatcga ttatggccac
     3961 accgagaccg cattcggcga tacgctgcag gccgtcagcg gtcatcgtta tgcgtcgatt
     4021 ttcgaacggc ccggtgagac ggatcttacg gcccacgtcg acttcgcaga gctccggcgg
     4081 ctgtgcatcg agcgtggctt gagatttgac ggaccgatca cgcaatccga gtttctgctg
     4141 ggcctcggtt tggcggaacg ggcgacgaag ctgatggcgg cggctcgatc tgacgaggtc
     4201 ggactgatag aggctggagc gcaccgcatc gccgacccgc tcggcatggg cggaaggttc
     4261 aaggccgctt gcgtgcgttc ggcgaacgtg ccggtgctgc cgccatttcc cgcagccggc
     4321 gcgaaagggg gataggctca tggctgtcga cggcgagatc attcgcgcgg aatgcctcag
     4381 cgatgcggcc ggcatcagcc acggcttctt cacgcggcag ggcggcgttt cgacagggat
     4441 ctacggggcg ctcaattgcg ggctcggctc caaggacgac ccggctctcg tccgggagaa
     4501 ccgggcgcgc gttgcccggg cgctcggtgc gccgcgcgag gaggtgacga ccctctacca
     4561 ggtccacagc gccacggcgc tcgtgatcga tgcacccttc ccgccaggcg ccgttccccg
     4621 cgccgacgcg ctcgtgaccc gcacgccggg gctcgtcatt ggtgcgatgg ctgcggactg
     4681 cacacccgtc ctcttcgctg atcccggtgc gcgcatcgtc gcggccgcac atgccggctg
     4741 gcgcggggcg ctcggtggaa ttctggaatc gacgatcgcg gccatggtcg gcctcggcgc
     4801 cgatcggagc cggattcgcg ccgccgttgg accgtgcatc aatcagtccg cctacgaggt
     4861 cgggccggag ttcgaggccg agttcctgcg cgccgatcct gagaatgcac ggttcttcgc
     4921 gcgaaattcg accggaacgc gcagtcattt cgatttgccc ggatacgtgg aacaccggct
     4981 gtcgcgggca ggattggcga tcgttgagcg gcgttcgccg tgcacttacg aaaacgaatc
     5041 acgtttcttc agctatcgtc gtgcgacaca ccgaaaagag cccgattacg gtcgccaaat
     5101 ctccgccatt gtcttgactt agcccttcat ccacagaaat caccggcaaa gtcgggcagt
     5161 aggggcacta cgcggcctgt cgtggggcaa acgatctgga cgccttgcaa gtcaggagtt
     5221 acacgatctg actcaagggt accggattct caaacttcag gccgcagggt tggtacgtcg
     5281 gctgccggat tgaacagcgg gacatcagag ggggacgtca tcgtgacgac catccatgct
     5341 gctgcggcgt cgcatcttgt cagacggctt gcgttcgcgg cgctggcgac agtcttcgct
     5401 gcgggcctgg gggcctgcag ctctgacacc aatattctcg gaaatgcggg ggatggctcg
     5461 cagctcctgt cacaacagac cgtgcagccc gccccgacat catcgcagat cgcgatcgcg
     5521 ccggtcatcg gcgcacctga gtccgtcggc aagcagatcg tcgctcagct gacgcaggac
     5581 ctggagcaga agaaggtcgc ggtggccaaa gcgccgaccg accgtgccga tttcacgctg
     5641 cgcggctaca tcgttgccgc caaggagaag aacggcacga aggtctcgta catctgggac
     5701 gtgaccgacc cgacgggtca gcgcgtcaac cgcatcaccg gcgaggaagt cgcaggcaag
     5761 acggattcgc gtgatccttg ggccgccgtt acgcccgagg tcatgcactc gatcacgagc
     5821 aaaacggcga catcgctcgg cacgtggatc cagtccgcga cggccgttgc cggttccggg
     5881 cagacggccc agccggtcca gacggccgcg cttggcagct cgaccgccgc ccaggcgtcg
     5941 tccgctccgg tggcgccacc tgctgcgacc gccagcatca gcaatagcgg caccgttaca
     6001 gccgtggtgc cgtccgtcac gggagcaccc ggcgacggct ccgtgtcgct caccagggct
     6061 ctgcaacggg agttgtcccg aaacggcgtt gcactggccg agggcccggc cggtaacgct
     6121 taccgggtcg aaggccgcgt gaagatgggc gccagtcaga acgggaagca gcccatccag
     6181 atcgactggt acgtgaaaga cccacagggc aagcagctcg gcacggtctc gcaaaagaac
     6241 gagatcccgg cggggtcgct cgacggcgag tggggcaagg tggccgatgc cgccgcctcc
     6301 gctgccgccc agggtatcct gaagctgctg cccggcaagg gcgtgaatta aactgctggc
     6361 agccgcttca cattgccgtc atcaaagcaa aggcccggac gtttacacgt ccgggcctgc
     6421 ttgctagaag ccgcctcggc gggacaatcg tggtggcgac gatgaacgat gctggccgga
     6481 agaaccattt cgacgggctg aagatcaagc tcgttgcagg caacagcaat cggccactga
     6541 ccgaagagat cagctcggtt ctgggcgtgc cgctcaccaa aggggtctgc cgccgcttcg
     6601 cggacatgga gatcttcgtc gagatccagg agaacgtccg cggcgaggat gtctacgtca
     6661 tccaatcgac ctcgtttccc gccaacgaca acctgatgga gttgctgatc ctcatcgatg
     6721 cgctgaagcg ggcgtcggcg cggcgcatca ctgcggtcat tccttactat ggatacgcgc
     6781 gacaggatcg gaaaccgggg ccccgcacgc cgatttccgc gaagctggtg gccaacctca
     6841 tcgagcgggc cggcgcaaac cgtgtgatga ccctggacct ccatgccggc cagattcagg
     6901 gtttcttcga tatcccaacg gataacctgt tcgccgctcc ggtcatggtg cgggacatca
     6961 aggagcgctt cgagctcgac aacctgatgg tcgtctcgcc ggacgtgggc ggcgtcgtgc
     7021 gcgcccgtgc cctcgccaag cgcatcggcg ccccgatcgc gatctgcgac aagcggcgcg
     7081 agcgtcccgg agagagcgag gtcatgaacg tcatcggcaa cgtccgcggc aagcgctgca
     7141 tcctcgtcga cgacatcgtg gacagcggcg gcacgctctg caatgcggcc gaggccctgc
     7201 tccagaacgg cgccatagag gtcatggcct acatcacgca cggcgtgctg tccggcggag
     7261 ccgtggcgcg aatccagaac tcgagcctca agtctctcgt catcaccaac tccatcgatc
     7321 cgacggaagc cgtgagggcg tcgaagaaca tccgcattct ctccattgcc ccgcttatcg
     7381 cagaggccat cgcccgcacg tcgcgtgagg aaagcgtctc gagcctgttc tactgacggc
     7441 cggtagaagc ggcgccctgc gggtcgcagc cggaatccgt cacattattg tcaacctcac
     7501 ctctcgcggg tataagcctc ggcatctcag tgacatgaga cgagactcct tcggcaccct
     7561 tggaggccgg ggacagcagc atgggccgtt tgcggccctt taacatttgg agaacagcca
     7621 tggctgaagt cgtcgagatc ccggccacgg cgcgtcccag ggccggtaag ggggccgccc
     7681 gggcagtgcg ccgtcaggga aaaatcccgg ccgtcatcta cggtgagaag caggagcccg
     7741 agacgatcgc gctcgatgcc aacgagcttt ggaaggtcgt gcaaaagggc cgctttctgt
     7801 cgaccgtgct cgatatcaat gtcgacggcc agaagcgtcg cgtcctgccg cgcgaggtgc
     7861 agctcgaccc ggtgaaggat ctgccgctgc acgtcgattt ccagcgtgtc ggcgcttccg
     7921 attacgtccg ggtggtggtc ccggttcgct tcatcaacga gggcgcttca cctggcctga
     7981 agcggggcgg cgtgctcaac gtcgtgcgcc acgaggtgga ggtcacctgc ccgccggatc
     8041 gcattcccga agccctcatc gtcgacctga cgggcctgga gatcggccgc tcgatccata
     8101 tttcggccgt gccgctgggc gagggcgtgc gcccgaccat caccaaccgc gacttcacag
     8161 ttgccaccat cgccggcaag aagaccgacg aggaccgcgc tgccgaggct gctgagggcg
     8221 ctgaagccga gaagaagtga tcgggcgggt gaagtcgtga agtggtgaga tcgtgaaatc
     8281 gtgaccagcg gctcgctgca aagcccgact tcacgacttg gctatttcac gatttcacgg
     8341 cccaatcaca gcaccttccc aggattgagg atcccgttcg ggtcgaaggc ggccttgatg
     8401 cggcgcatca ggtcgatctc gacgggactc ttgtagcgga cgaggtcggc gcgcttcatg
     8461 cggccgatgc cgtgctcggc ggagatcgag ccatccatct ccgccaccag cgcgtgcacc
     8521 gcactgctga tcggctccca tagctccagg aagcgctcct tctccatgcc gatgggctgg
     8581 gcgatgttgt aatggatgtt gccatcgccg aagtgcccga gcggcagtgg ccgcgcgccg
     8641 gggcaaatcg actccacgat ccggtcggcg cgctcgatga actccgcgat ccgggcgatc
     8701 ggcacggata catcatgttt gatgttgccg ccttcgggct tttgcgattc cgacacgctc
     8761 tcacgcaggc gccacagctc tcgcgcctgc gacagcgagc tggcgagaac ggcgtcgagc
     8821 aacaggccgc gctccgtggc ttgcccgagc acgttttcca tgtcccgcgc cgcctgcccg
     8881 tcgggctttg cccctgaaat ttcgatcagc acgtaccacg ggtatttctg cgggaacggt
     8941 tcccgcgtcc cagggacgtt gcggagcacg aaatcgatgc agatgcgggg aatgaactcg
     9001 aaggcggtga cggaccggcc cgccgtctcc tgagccaggg agaagaggtc gagtgcatga
     9061 gagattttgg ccagtgcgac catcgctgtc gcactctcgg ctggccgcgg gaatagcttc
     9121 agcaccgcag ccgttatgac acccagcgtg ccctccgagc cgatgaaaag gtccttcagg
     9181 tcgtagcctg tgttgtcctt tttcagcgct ttgagggccg ggacgatgcg gccgtcggcg
     9241 agcaccacct cgagccccag gacgagcttg cgcgcattgc cgtaggccag aacgccgacg
     9301 ccgcccgcgt tggtagccag gttgccgccg atctggcagg tgccttccga cggcaggctc
     9361 aggggaaaca aacgatcgac gcgttcggcc gccgcgtggc attcggcaag cgtcaggccg
     9421 gcctcgacgg tcatgtgata ggctgcggga ttgatctccc ggacccgctt gagccgcgat
     9481 acggacagaa cgatctccgt tccagtctcg aacggcgtct gcccgcccac gagacccgta
     9541 ttccccgcct gcggcacaac cgcgacccgg gcctcgttgg ccagcgccag aatgcgcgat
     9601 acctcctccg tcgtccccgg cctcagcacc accggactgc gcccgatgaa cagatcgcgc
     9661 cattcgcgca aatatgggag ctgctggtcg ggatcggtca gcgcatgctc cggtcccacg
     9721 atttcggtca gtttcgcaat cagctccggt gtgggggcgg ccagctccgg gcgttttggc
     9781 tcagtcatga caagatggtc caggtcgtgt gttggtgtct tttgccgggc gaacgtgtat
     9841 catactgccg cagctttgac agaggcggcg tggccagcat ttgccaaaga ccgccctttg
     9901 ccgcccccca ttgacgccct tgttcttcaa gtgtcatctg gccctgccat gtaccacgcc
     9961 acctctcgcc ccttagtcat gagtttgccc aacattctga cctacggccg catcattgcc
    10021 gtcccaggtc tggtggcgtg cctcttcttc ctgcatggcg acctggcgcg ctggtcggcg
    10081 ctcgttctct tcgtcacggc cagcctgacg gattggctcg acggatatct ggcgcgcgct
    10141 tggcagcagc agtcggcgct tggacggatg ctcgatccca tagcggacaa gctgctggtg
    10201 ggcacgacgc tgctcatgct cacccatttc aacacgatct atggctggca tgtctgggcg
    10261 gcggtgatca tcctgtgccg cgagatcctg gtctcgggcc tgcgcgaata tcttgccgag
    10321 ctgaacgtga agatccacgt cacgcaactc gccaagtgga agaccacttt acagatgatc
    10381 gcgctgggag tgctgcttgc cgggcctgcg ggtgacaagc tcctgccggt ggtcacggcg
    10441 agtgggctcg cgctattgtg gctggcggcc ttgctcactc tgtggactgg ctacgattat
    10501 ctgcgtgccg ccgtgcagca tgccatcggc ggcagcgaat gagttctcgt accatggctg
    10561 aacagaacac cggcggaacg acagtcaagc tcctctactt cgcctgggtg cgcgagaagg
    10621 tcgggcgtgc cgaggaggca atcgaactgc ctgccgggat cgaaaccgtc ggggagctgg
    10681 tggggtggat gaaatcccgc gggcccgagt acgcggccgc cttcgagcgc tccggcgtga
    10741 tccgggcggc aatcgaccag aagcacgtgc ccccctcggc cagcatccgc ggggcacggg
    10801 agatcgcctt tttcccgccg gtgaccggag gctgacacat ggccgttcgc gtccagcgtg
    10861 aggacttcga cgtaggcgcg gaaatcagcc gcctcacgac ggggcggtcg gatatcgggg
    10921 ctgtggtgac gttcaccggc ctcgtgcgcg gcaactccac gggccggccg gtcgcggcta
    10981 tgaccctcga gcactacccc ggcatgaccg aggcggagct ggcccgcgtg gaagccgagg
    11041 ctcaggcccg ctggccgctg caggcgagcc tcatcatcca tcggatcggc gagctgaagc
    11101 ccggcgacaa catcgttctt gtcgtcaccg cctccgccca ccggcacgcg gcattcgagg
    11161 ctgccgagtt cctgatggac tacctcaaaa cccgcgcgcc cttctggaaa aaagaagccg
    11221 acccggacgg aaggggcagc tgggtcgatg cccgcgagag cgacgaagcg gcagctgagc
    11281 ggtggcactc ttgagcgcag cgaccggttc ggcctctcat cgacgtattc gcgagatccc
    11341 acgttagagc atttccgagt tagacagaat caggccaagc caagctcgcg cccaatctcc
    11401 ctctccccgg cggggagagg gtcggggtga gggggttccg ccggtggcag ctgttgtaac
    11461 ccctcacccg gcccttcggg ccgacctctc cctatgggag aggttaacgg agtgcttggt
    11521 gttggcgttt ccatctgaat cggaaaagct ctaggcgacg tgcagcatcc tgcgttcgct
    11581 gtttccgcac gcctcgtcac cggccgccaa gatcccagta atcgccgcgg aaatagagca
    11641 gcggctccgc ctctgcgcgt acggtgccgt cctccacaga gccgatgaaa atcgaatggg
    11701 tcgcgaaacc gtgctgctct tcgagccggc agtcgaggct ggcgagcgcg ccgcgcagga
    11761 ttggtgctcc ggtggcgagt gtcgtccagt cgtcctcctt gaaccggtct tcgccgctca
    11821 gccccatgcc gcctgaaaac agctctgcga tcgcttgctg atccgaggcg aggaggttca
    11881 cgctgaacac accgagccgg tggatgacgt cgtgcgtccg agccgttcgc cggatgcagg
    11941 cgagaatggt cggcggatcg tctgacaacg aacagacggc cgtggcggtg agcccgctcc
    12001 gccggcccgg cggcccgacc gcaatcacgg ttacggcgcc cgcctgatgt cgcatgatgg
    12061 cgcgataaag agctccatcc atttacttga tccctgcgtg cgcagagcgc acggctcgaa
    12121 gcgcgcgccg cctcacggct tcgatcggcc tgcgagccaa cgcttcagat tttcggccag
    12181 cgccgattgc cgctttccca tgcgttcgcc ggccggatgc agcggccgcg gctgggtcgc
    12241 aggaggcttg gcctttgcct cctccggcga gggggcctcg cgcgtggcct cttgcatggc
    12301 gcgcgcaacg tccgtgagga agcgctgggt atcgccgctg gcgaggcggc cggacgcgtt
    12361 cgcgatggca tcgagcagcg ggtccagcca ctcctggtcg gccttggcga agtcgtggag
    12421 cacgtaggga atgacggcgt cctttgagcc cggatggccg atgccgatcc gaacccgtac
    12481 gaagtcgttg ccgagatggg cactgatcga gcgcagaccg ttgtggccgg cattgccacc
    12541 gccgaccttc accttcactc tgccgggggc aaggtccagc tcgtcgtgga agacaaccac
    12601 gtcctgcagg tcgattttga agaatcgtgc cgcttcgccg accgcgcgcc cgctctcgtt
    12661 catgtaggtc atgggcttga gcagcaaaac gcgctcgccg ccgatggtcc cttccgccgt
    12721 ctcgccctga aaccggcggc gccatggcgg gaacctgtgc cgctcggcaa tccgatcgac
    12781 agccatgaag ccgatgttgt ggcgatggcg ggcgtacttg tcgcccggat tgcccagtcc
    12841 gacaaaaagc ttcatgacgc gccgatctcg cacatccgcc gatgagccct gactgcgggg
    12901 aatcgtctat ggaactgcat ggctcagctt atcgatcccg tctcgacgtc ctgcaagccc
    12961 gcatttagcc tcgccagatg tttcaggcgg caggggaccg ttctacaatg accgcaaccg
    13021 ggaagacccg agttacagag ccgtgctggc caaaggccgc ggcgagtgcc gcaatcttcc
    13081 gcggcaacgc ggtcctcctc gtcgagcggg gcaagccgcc cgtcagcggt gtctggagcc
    13141 tgcccggcgg tcacatcgag ccaggcgagc ccgcacggga tgccgcgctg agggaagtca
    13201 cggaggaaac cggcatccgg gccgagctcc tcggactggc cgacgttcat gacgtgatca
    13261 tccgcgacga ggcaggagcg cttcaggccc actttctgct cgcggtattc tatggccgtt
    13321 ggctcgccgg cgagcctgcc gccgccacgg actgccgcga tgcccgtttc gtgccgctgg
    13381 atgagattgg aacctatccg ctgaccgacg gcgcccagcg catcatccaa aaggccgccc
    13441 ggctgctaag gggttgaacg agggcgttca gcccgacacc acggtttaac tttcaagcct
    13501 cccgcgcccg gccgatcatc agcatctcgg agaaattctc cggcgtgatg aacccgagca
    13561 ggcgctcgtc ggggccggtg acagcgacgg cgcgggcgct cttctgctgc atcatcgtga
    13621 cgacgttgtc gagggtcgag tgcccggaga ccacgggaat gtccttgtgc atgaactcgg
    13681 tgacgagcgt gctgccgccg cgatcgcgca aggcctcgat gagcacgtcg cgggtgacga
    13741 tcccctgcaa ccgggaattc ccgtcgagca ccggaaactc ctgctgggtc gtgcgcagga
    13801 ggagcgaagc cgcatcgtcg gcggtggacg tcgggcccag cgcctcgaac ttggtgatca
    13861 tcgcatcgcg cgcgagaaag ccgcgggcga gcgagcgcat ctccacatat tggctttcgg
    13921 ccgtcgcggc gaagtacacg aaaattgcaa tcagaatgag cagcgggttc ccgaacaggc
    13981 cgacgaagcc gaggaagatt gcaaacatct gcccgatgct ggccgccgtc cgcgtggcgc
    14041 gcacgaaccc catcttcatt gcgagcagcg cgcggagcac gcggccgccg tccatgggaa
    14101 acgcggggat caggttgaac acgaccagcg ccacgttcgc aaccgcgaca cgggccatca
    14161 tgttcgtccc cggattttgc tctaggtcca gtgctgacgg atcgatgcgc gcgcccagga
    14221 tggcgatgag cacgaaggcg atgaccacgt tcaccgctgg cccggcgagc gctacgatga
    14281 cttcctggct cggcttttcc ggcatccgct ccatgctggc gacgccgccg atcggaagca
    14341 gcgtgatggt cggagtcttg atgccgtacc gccgggcggc aaagatgtgc ccgaactcgt
    14401 gcagcagcac gcacaggaac aggaggatga tgaagatgat ggcgaagacg gccgcttccg
    14461 gcccgaccgc catccactga atggccccga accaggcaag gagcaggaaa aaggtccagt
    14521 ggacctggac cagcgtgccg ccaagcctga acaatgggaa cgaccagctc atgtggaacg
    14581 tctctccgca gatgaccgac gaactcgatg gttgctcccg gcaacgctcg aggctttgac
    14641 ccaggtcaca tttagttgct gttccgtcaa atgcgagcgg ctcacccctg acaaccgctt
    14701 gagcgaacgc gcgttccgct actgcacaag ggcccagcgc ccttgatcag aagtggaacg
    14761 aggacctgac cgctcgagcc tcgccattat gccacgccgc gctttcgacc cgcgcgtcac
    14821 attcctgctc ggtcagacgc ataccaaccc acgccccgcg gaagcgggcc gggtcacacc
    14881 gtcaccagcg gaacagcggg tcgtctgcag acagcttagc ctgatgcagc tcgcaccagt
    14941 cgaaaggttc ttcaatgctt ctcagccgcg atgcgatccg ccacgtccct gccctcccga
    15001 tcgacgtatt cgtcgacttc gacggcacca tcgcgccgga tgatccgacg gactcgctgt
    15061 ttgcgaggtt cgccgatccg gcgtggctcg acatcgaggc cgagtggcag tcaggacggg
    15121 tttcctcgcg cgaggcgatg gcgcggcagg tggggctcat tcgggcaacg cccagcgaga
    15181 ttgcccgact tatcacacag atgaagatag acccgggctt tccggagttc gtcgcgctgt
    15241 gccgtcgcca tggcgcgcgt atcacggtgg tatccgacgg gttggacctg ctggtcgagt
    15301 ccatgctgaa ggcggccggt ctcgatctgc cgttctatgc caatcagctc gtctggcagg
    15361 gcggcgaccg ttgggccctg cgcttcccgt acatacgatc cgactgccgc atgcgcatgg
    15421 gcaattgcaa atgcgcgcat gcgcggttca tggggatcgg cgccaacgtc atggtcggcg
    15481 atggacggtc ggacttctgc atcgccgagc gctgcgatct cgtgctggcg aaaggcaagc
    15541 tcgcgcccca ctgccgggaa aatggcgtgc agcatatccc catccgcaac ttctctgagg
    15601 ccacctcggc catgctcgac tggctgaccg ggatccgcag tggcctgacg gcctgaacct
    15661 agctgtccgg cattcccaga gattttgcaa gccgcgcctg agcggccagc cggatcggcg
    15721 cgttcaccgc gccgaagccg acatatcggc gccgctcgac gatctcgaag aagaaccgct
    15781 cctggaagct cggcgtgtag atctggaagt actccgcgct gccgtcccgg tcgtaaagga
    15841 tgttgctggc cttcagccgt gccagctcat ccgcggtcag atcccagcgc gcttccagat
    15901 catcgtaata gttctcgggg atcggcagca gctcgacgcc gttctccttc agcttttcgg
    15961 ccgtcgcata gatgtcagcg caggagaacg cgatgtgctg cacgcccgag ccgaagaact
    16021 cctctatgaa ccgggacgac agagtccggt tcgattgcga gccgttcagc acgatccgca
    16081 gggcgccgtc cgcggtttcg acgacctggc tccgcacgat gccacccggg tcgatgacgt
    16141 cctggcctgg agtcttcgtc acgtcgagca gcgacgtgta gaagagcagc catgagagca
    16201 tctcctcgta gtgcatggac tgcgagatgt gatcgacggc gaggagcccc gcatcgcggc
    16261 tgttgacagg ctccgcagga ggcgtgaact cgatctccca cacgtcggcc aacggcttcc
    16321 gtctgtccag gaaatagatc aggctgccgc ccaatccgcg cgccgccggg atttcaagct
    16381 cccctgcccc gaccgcctgc cggaacggcc gggcgagcag cttcttcgcg cgctcaagcg
    16441 tgcggcccgc gtcctccaca tcgaggccta tggcgcacac ggatgtgcca tgggtgatgt
    16501 tgaacgagtg agcaaagccc tccttctcgg agttgacgac gagattgata tcaccctgcc
    16561 gccagcggac gacagatttc gacttatgga tgccagcctt aacaaatcca agctgaccga
    16621 aaagtcggga aatcgcaggt gccgtcacct cgtcgacggc gaactcgatg aatgcaatgc
    16681 cgtgacaggt tgacctgggc ggcagctccg ggaggccggc gatcggcgcg cccgtgtgct
    16741 gccgaagctg atcgaggagg tagagcagcg aacgctgacc atcggtcgcc accatgcgcg
    16801 ccgagccggc gcgaaactgg tcgttgaaga tttcgagaga gatgaggccg tcaaaatctg
    16861 tcgcgtgcag cgcctccatg aaatccatga gcggcagctc gccctgcccc ggcaggcagc
    16921 gcaaatggcg gctccaggag agataatcca tctccaattg cggcgcatcg gcgacctgga
    16981 caaggaaaat ccgatcccgc gggatggagc gcatcggctt cagatccgtc ccacgcgcca
    17041 ggatgtgaaa cgagtcgagg atcagtccca cggctgggtg atcggtgcgg cgcaccacct
    17101 cccaggcgtc gcggtaatca ttgatatggc gaccccaggc cagcgcctcg aagccgatgc
    17161 ggatgccgcg ccgggcggcg cgttcgccga gctcacgaag gtcggctgcg gcgcgatcga
    17221 tgccgccgat cgattccggg gaaacgttgc tgcaaacaag gagcagatca cagccgagtt
    17281 gctccatcac atcgaacttg cgctccgcac gggcaaagtt ccgcgcgcgc tgcggttccg
    17341 gcatcccctc gaagtcgcgg aagggttgga aggttactgt ggagaggcct aggtctgaca
    17401 ccatctggcg gacatcggcc gtcgtgccgc tgaacgagag gagatcgttc tcgaagatct
    17461 cgaccccttt gaagcccgcc gcggcgatcg cctgcagctt ttcgtcaagg gctccactga
    17521 ggcagacagt agccattgcg gtctgcatgt ctggcacctg ctcgcgaaac gctcccggga
    17581 aacaggctcg cacaatttgc tcagttgaca agctgttcct gtgctctgcg gaaacgctcg
    17641 ggagcggcct gcgttcgctt ccaagcagcc accacatcaa tgagttgtct catgcccagc
    17701 tccaggcatg acccgaggca gtcgaaagtc ccgacggaac acactgaccg caagggcgcc
    17761 gtgcgcggcg cgagaatgcg atcggattgc ggttgatccg gtcgcatgtt aacgattagt
    17821 ggggcttagg gagttcggct cacacgccgt cgccgcggat aacatgacgc ggaggatgcc
    17881 ccatgtcact ggcgaaacag gttttcgcgc tttccctctc cctcgccgcc cttggctaca
    17941 cggtcattcc ggccgtccag gctgccgtca aacccggacc aggccctctc gattcccaga
    18001 gcatgatcgt tcaggtgcag aagaaggcca aaggcaaggc cacctgcggc acctacatgt
    18061 actggagcgc aaaagagcgg aaatgtgtgg acgcgcggct caagaagtcg aaagggagct
    18121 aacccatttt attcacgatc cagtgcggga tgagatgatg ggcggggacg aagccgttgg
    18181 tttggcgtag cattctggtg tcgaagcaga gtgcgcgtcg ccagagccca ccacaccgcc
    18241 catcgccatg catgctacgc cgttcctgcc cggcctgtca ccggtcggat caaagccgct
    18301 cacagccacg ttcgatgctg gacgcttgtc ctcggatggc ggcctgatcg tgttgcgtga
    18361 gatcgcaact cgcttgcggc ttgccgagac gatcaccaac ccgctccgcg acgcgcgcga
    18421 tccgacccgc gtccgtcaca gctacgccga gatggtcgcg gcgcggatgc tcatgatcgc
    18481 ggccggctat gaggactgcg acgacgtcga tacgttgcgc accgatcccg cattgaagat
    18541 cgccgtgggt cgggcgccgg agaccggcgc cgagctgatg agccagccga cattgtcgag
    18601 actggagaac ctggccgact ggcgcgcatt ggccaggatc ggtctcggcc agatcgatct
    18661 ctactgcagg agcttcgctc gcccgccggc gcgcatcgtg ctcgatatcg acgacaccga
    18721 tgacccggtg cacggccagc aggagctggc gctgttcaac gcgcactacg actgcacctg
    18781 cttccagccg atcctgattt tcgacggcct ttcgggcaag cccgtgctgt cgctgctgcg
    18841 ccccggcaag cgcccgtcgg gcgaggaggt ggcgaaggtg ctgcgccacg tgatccgccg
    18901 catcagacgg cactggccgc atgtcgcgat cctggtgcgc ggcgacagcc attactgcag
    18961 cgagccggca ttggcgctgc tggaggctat gcgctgcgac tacatcatcg gcttcgccat
    19021 caactccagg ctgctggaga tcgccgcccc ctggcgcgcg caatgcgaca tgcgccgctc
    19081 aaggatgaag ccgaccgtgc gccgcttcca tcagcttccc tatcgggccc gcgagtggag
    19141 ccggtcgcgc aagctcgtcg cccgcgtcga agcgacaagc cttggcaccg acgtgcgctt
    19201 tatcgccacg agcctcgaag gtcgcgccaa gacgctctac gagaaggtgt tctgcgcgcg
    19261 tggcaaggcc gagaacctga tcaaggacat gaagcgcttc acgcgctccg acaagacggc
    19321 gtgctcgcgc tggcaggcca atcagttccg cct
//
LOCUS       DASVDU010000201         4741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_570340, whole genome shotgun sequence.
ACCESSION   DASVDU010000201 DASVDU010000000
VERSION     DASVDU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570340"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VF226_12970"
     CDS             <1..418
                     /locus_tag="VF226_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7074945.1"
                     /translation="KSFRWIPNGIPGVETRLPILFSEGVKKGRIDINRFVALTATNHA
                     KLYGLYPRKGTIAIGSDADIVLWDPERRLTIAQSLLHHGADYTPYEGFDVVGWPTLTM
                     VRGRTVVKDGRLIGSPSHGIHLQRRARGSGLSTMFA"
     gene            complement(419..1183)
                     /locus_tag="VF226_12975"
     CDS             complement(419..1183)
                     /locus_tag="VF226_12975"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu/hydantoin racemase"
                     /protein_id="HEX7074946.1"
                     /translation="MTSAHAGFGPLTSLKKIGHITPSSNTAVEPMTALLNASLADRVS
                     HHFTRIRVTQITLGEGTASQFEFDRMLAAAELLADAPLNAIVWNGTAASWLGIERDEA
                     LCSEITDRTGLPSSTATLAFHAAFQQFGFRKIALAVPYTGEVTEQIAGVYAQRGIQVV
                     SSAHLGQSVNAEFADNGPDPIRALLRDADSDAAECIAVVCTNFPATPLVDEMERELRK
                     PILDSIAVTFWQACRLAGVTPQLKGWGALMGGALQP"
     gene            complement(1211..2497)
                     /locus_tag="VF226_12980"
     CDS             complement(1211..2497)
                     /locus_tag="VF226_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7074947.1"
                     /translation="MIGVTILILLGLLALAVPVAAALGVLGLSLSALYSPLPLSLAMG
                     EMAWATSNNFLLVAIPFFVLLGEILLRSGIAERMYNALVLWMPWLPGGLMHSNIAACA
                     MFAATSGSSVATAATIGTVATAEIEKHGYSERLFLGTIAAGGTLGILIPPSINMIVYG
                     VLTDTSIPKLYLAGIIPGLILASFFSLTVLIICLVRPEMGGVPTQANWKARIKALPDL
                     IPPFVIFLAVIGSIYAGLATATESAALGVIAALGVAAWHGRLTAAMLKAAFEGTMRTT
                     AMIMAILIAAYFLNFVITAIGLTSQVNSTITQLDLSPLQLLMMVVVFYIVLGMFMETL
                     SMMVATVPIIAPVVVGAGFDPVWFGVIVVILLELAMITPPVGINLYVVQGLRKRGNIN
                     DVIVGAAPFVIAMFAMIIALTIWPQLALWLPGITSH"
     gene            complement(2494..3081)
                     /locus_tag="VF226_12985"
     CDS             complement(2494..3081)
                     /locus_tag="VF226_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7074948.1"
                     /translation="MAGIRHFSRAGLWLGGALILLAAILIAIDVVLRAGFNTSIGGAD
                     ELSGYALAIGTAWALGAALLDRAHIRIDSLYSLFALRLRLVLDVFGIVLFTAFFGLVG
                     WHGIGVFTQSWVAGSRSQSALEVPLVIPQAAWLVGLAVFLVTALMLLAHALTLGVRGD
                     LVEMSRTISTRSAAEEIDDEVRALAEQRAREARAQ"
     gene            complement(3390..4439)
                     /locus_tag="VF226_12990"
     CDS             complement(3390..4439)
                     /locus_tag="VF226_12990"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7074949.1"
                     /translation="MSIRLLAGVVAAVSLALPVAAQELPKSHFKVIGLNSPTPVSIYD
                     ELPFWRETIPEASKGAITAEVTPLDQMGIDDKTMLRLLKLGVMDFAGMDISKMAGDDP
                     RFEGCDLAGISLTPDKARAACDAYRDVIDRQMQKNWNAKLLAFGGNTPQVFWCRGEVN
                     GLDDLKGKKIRVFNNTTRDFLSGLGATAVSMAFAEVVPALNNGVVDCAVTGSLSGNTA
                     GWWEVAKSLYNMSLGWSINVLAVNLNTWNRLDPKTQAFLEEQIKAYEDKMWDTLKKST
                     EEADNCNTNKQPCTLGKLANIKLVQVSPEDAETHKKLVEGAVLAGWAKRCGAECAKEW
                     NETVGKALGLTAPIP"
BASE COUNT          941 a         1460 c         1481 g          859 t
ORIGIN      
        1 aaagagcttc cgctggatcc cgaatggcat cccgggcgtg gagacgcggc tgccgatcct
       61 gttctccgag ggcgtgaaga agggtcggat cgacatcaac aggttcgtcg ccctgaccgc
      121 caccaaccat gccaagctct atgggctata tccgcgcaag ggcaccatcg ccatcggctc
      181 ggatgccgac atcgttctgt gggacccgga gcggcggctg acgatcgccc agtcacttct
      241 gcaccatggc gcagattaca cgccctacga agggttcgac gtcgtcggat ggccgacgct
      301 gaccatggtt cgtggacgaa ccgttgtgaa ggacggtcgt ctcatcggct cgcccagcca
      361 cggaatccac ctccagcgac gcgcgcgggg cagcggactg tcgacgatgt tcgcctgatc
      421 agggctggag agcgccgccc atcagcgcgc cccatccctt aagctgcgga gtaacgccgg
      481 caagccgaca ggcttgccag aaggtcacgg caatggagtc caggatgggc ttgcgcagct
      541 cgcgctccat ctcgtcgacg agaggcgtag cgggaaagtt cgtgcaaaca acggcgatgc
      601 actccgcggc gtccgagtcg gcgtcccgaa ggagcgcccg aatcggatca gggccgttgt
      661 cggcgaattc ggcgttgacg gactggccga gatgggcgct cgaaaccacc tggatgccgc
      721 gctgtgcgta gacgccggca atctgctccg tgacctcccc ggtatacggc acggcaagcg
      781 caatctttcg aaagccgaac tgctggaagg cggcatggaa ggcgagcgtt gcggtcgagg
      841 atggcaggcc cgtccgatcc gtgatctcgc tgcaaagcgc ctcgtcccgc tcgattccaa
      901 gccagcttgc cgccgtgccg ttccagacga tggcgtttag tggcgcatcg gccaagagct
      961 cggcggcggc gagcattctg tcgaactcga actggcttgc cgtaccttcg ccgagcgtga
     1021 tctgcgtcac gcgtatgcgc gtgaaatggt gagaaacgcg gtctgccagc gaggcattga
     1081 gcagcgccgt cattggttcg acggcggtgt tcgacgaagg ggtgatatga ccgatcttct
     1141 tgaggcttgt cagcgggccg aagccggcgt gggctgatgt catgtcttgt cccgcatcga
     1201 ttggctgcca tcagtgcgag gtgatgccgg gcaaccataa agccagctga ggccagatcg
     1261 tcaaagcgat gatcatcgcg aacatggcga tcacgaacgg agcggcgccg acgatgacgt
     1321 cattgatgtt tccgcgcttc cgcaacccct gcacgacgta gagattgatg cccaccggcg
     1381 gtgtgatcat ggccaattcc aacaggatca cgacgatgac gccgaaccag accgggtcga
     1441 agccggcgcc cacgacgacg ggcgcgatga tcgggacggt cgcgaccatc atcgacagcg
     1501 tttccatgaa catgccgagg acaatataga acaccacgac catcatgagg agctgcaggg
     1561 ggctcaggtc gagttgcgtg atggtgctgt tgacctggct ggtcagcccg atcgcggtga
     1621 tgacgaaatt gagaaaatag gccgcgatga gaatggccat gatcatggcc gtcgtccgca
     1681 tcgtgccttc gaacgccgct ttcagcatgg cagcggtgag gcgcccgtgc catgcggcaa
     1741 cgccgagggc agcgatcacc ccaagggcgg cggattccgt tgccgtggcg agccctgcgt
     1801 aaatggagcc aatcaccgcc aggaagatca cgaacggagg gatgagatcc ggcagcgcct
     1861 tgatccgcgc tttccaattg gcttgcgtcg gtacgccccc catctccggc cggacgaggc
     1921 agatgatcag caccgtcagg ctgaaaaagc ttgccaggat caggccagga atgatgccgg
     1981 ccagataaag cttcggaatc gaggtatccg tcagcacgcc gtagacgatc atgttgatcg
     2041 acggcgggat cagaataccg agcgttccgc cggcggcgat ggtcccgaga aacaaccgct
     2101 ccgaatagcc gtgcttttcg atctccgccg tcgccacggt gccgattgtc gccgcggtgg
     2161 caacactcga gccggatgtc gccgcgaaca tggcgcaggc ggcgatattc gaatgcatga
     2221 gcccgcccgg cagccatggc atccagagca ccagggcatt gtacatccgc tccgcgatgc
     2281 cggaccggag caggatctcg ccaagcagca cgaaaaatgg gatggcgacg agcagaaaat
     2341 tattggacgt ggcccacgcc atttccccca tcgccagaga gagcgggagc ggcgaataga
     2401 gcgccgaaag cgacaggccg agcacgccta gggcagccgc caccggaacg gcgagcgcca
     2461 gaaggcccag caagatgagg atcgtgacgc cgatcattgg gcccgcgcct ctcttgcccg
     2521 ctgctctgca agagcgcgca cctcatcatc gatttcctcg gccgcggacc gggtgctgat
     2581 cgtgcgggac atttcgacga gatcgccgcg cacgccaagc gtgagtgcgt gcgccagcag
     2641 catcagcgca gtgacgagga agacggcaag cccgacgagc caggcagcct gggggatgac
     2701 aagcgggacc tcgagagccg actggctgcg cgagcctgcg acccacgact gcgtgaagac
     2761 gcctatccca tgccagccga cgaggccaaa gaatgccgta aacagcacaa ttccaaaaac
     2821 gtcgagcacc agccggaggc gcagcgcaaa aagtgagtaa aggctgtcga tccggatatg
     2881 cgcgcggtcg agcaatgccg ccccgagtgc ccaggccgtg ccgatggcca aggcgtagcc
     2941 tgagagctcg tctgcaccgc cgatggacgt attgaatccg gctcgcaaga cgacatcgat
     3001 tgcgatcaaa attgcggcaa gcagaatgag tgctccgccc agccagagtc ccgctcggga
     3061 aaagtggcgg atgcccgcca taagcgtctg catcggcgtg cccggtcttt tggcttttcc
     3121 gaattaggtg gaaacggcgg tcattctctt cacctctctc gtggggagag gtcggtccga
     3181 agggccgggt gaggggttgc aacaggcgcc actggcggcc cccctcaccc caaccctctc
     3241 ccctccgggg agaaggagag tgagtgtgag cctagcctga attgtttcca tctacatcgg
     3301 gaatgatttg gcctctttga tttatcgaga cagcctgccg gtaccgcgag tgcggccccg
     3361 cgataccggc gaagcccgca gccctcgctt tacggaattg gagccgtcag cccgagagct
     3421 ttaccgaccg tctcattcca ctccttggcg cattcggcac cgcagcgctt ggcccaaccg
     3481 gcaagcaccg cgccctcgac cagcttcttg tgggtttccg catcctccgg ggagacctgc
     3541 acgagcttga tgttggccag cttgcccaac gtgcagggct gcttgttggt attgcagttg
     3601 tccgcctctt ccgtggactt tttgagggtg tcccacatct tgtcctcgta agccttgatt
     3661 tgctcctcaa ggaaagcttg ggtcttggga tcgagacggt tccaggtgtt gaggtttacg
     3721 gcgagcacgt tgatgctcca gccgagcgac atgttgtaga gagacttcgc cacctcccac
     3781 caacctgccg tgttgccgga gaggcttccc gtcaccgcgc aatcgacgac gccgttgttc
     3841 agcgccggaa cgacctcggc aaacgccatg ctgacggcgg tcgcgccgag gccggatagg
     3901 aagtcacgcg tcgtgttgtt gaaaacgcgg atcttcttgc ccttgaggtc atcgaggccg
     3961 ttcacctcgc cgcggcacca gaagacctgg ggcgtattgc ctccgaaggc cagcagcttg
     4021 gcgttccagt tcttctgcat ctgcctgtcg atcacgtccc gataggcatc gcaggcggcg
     4081 cgcgctttgt caggcgtgag agagatgcca gcgagatcgc aaccttcgaa acgcggatcg
     4141 tcgcctgcca ttttggaaat atccatcccg gcgaagtcca tcacgccgag cttcagcagg
     4201 cgcagcatgg tcttgtcgtc gattcccatc tggtcgagcg gtgtgacctc agccgtgatc
     4261 gcgcctttcg aagcctcggg aatggtctct cgccagaacg gcagctcgtc gtagatcgac
     4321 acaggagtgg ggctgttcag accgatgacc ttgaaatggc ttttcggcag ctcctgcgcg
     4381 gcgaccggca gcgccagcga gaccgccgcg acaactccgg ccagcaatcg tatcgacatc
     4441 gggcattcct ccttttgacc tgacgccaaa tttccgcgta ttggctccgc tcccgaaagg
     4501 gaattgcaga ggctatgtca caccattcac gcaaggttga caatgccgat cagtgccctt
     4561 tcgttgggca atcgggtgct gaattttgat ctcactcgag ccgagaccgc ttggccggtc
     4621 agtaataatc ttgcgcggcc tcggcagtca catagccctg ctccaagtcc cactggacgg
     4681 cgtcgcgcgc gcgttccttt ggattcccgt ggcctccgcc gccggacgtc tcgagccgaa
     4741 t
//
LOCUS       DASVDU010000202        15466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1612175, whole genome shotgun sequence.
ACCESSION   DASVDU010000202 DASVDU010000000
VERSION     DASVDU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15466
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1612175"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2218)
                     /gene="bamA"
                     /locus_tag="VF226_12995"
     CDS             complement(<1..2218)
                     /gene="bamA"
                     /locus_tag="VF226_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7074950.1"
                     /translation="MGGWRLCLSVAAISVSASALTSFAAVPAVQAQGVIVREIKVTGN
                     RRVEPETVRSYLHFTVGDPYDAGKADSSLRALFATGLFADVEIERQNSTVIVHVVENP
                     VVNQVAFEGNSEIDDKTLEAEVQLKPRSVYTRARALADAQRILDVYRRQGRFAAVVEP
                     NLIELDENRVNVVFEINEGIATKVKAIHFVGNHAFSDSQLRDIITTTQSGWFDFLKGT
                     AIYDPDRMNLDRELLRQYYLKNGYADVNIISANAELDRDGTGFILTFVIEEGQPYVFG
                     DVRIDSQLPELHPETLNDELLTRPGETFNASYIDKTVEKLTLRTSELGFAFARVRPRA
                     DRNPAARSIDITYEIDQGPRVYIERINVVGNLRTKDRVIRREFRLAEGDAYNPLLIDQ
                     AKKRLQALGFFKAVEIQRRPGSAPDRVVLDVEVTEQPTGELSFGAGYSTNEGVIGDVS
                     ITERNLLGNGQFLRLALGGSVERFQIDLSFTEPRFLDRNIAAGFDLFHKEIDQSDQSS
                     FKSRRTGGSLRMGFPLAENFWMSTSYTLSHDDIFDVDDRASEVVKEAEGAALTSALGA
                     AITYDTRNHPRNPNRGLYFQVGSDFAGVGGDVQYVRVQGEGRAYYPITDEITFVGRVI
                     GGHIEGWGGQDVRLLDLYYRGGETVRGFDRAGYGPRDIDTGDALGGKTYWAATAEVRF
                     PLPLIPDELGMSGAVFADAGSLFGAGARAKELDAECNNADPNVRRVCLADDASIRSS"
     gene            complement(2454..3614)
                     /gene="rseP"
                     /locus_tag="VF226_13000"
     CDS             complement(2454..3614)
                     /gene="rseP"
                     /locus_tag="VF226_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX7074951.1"
                     /translation="MALLSSFADGIGGFLFVLVSFLFVLTVVVFVHELGHFLVARWCG
                     VSIKAFSIGFGREIFGFFDRHGTRWRISWIPLGGYVKFMDDENAASAGPSARLEEMTP
                     EERAGAFHAKPLWQRAAVVAAGPIANFLLAIVIFALMFSLIGVRSTAPRVDEVVPNSP
                     AAVAGFLPGDVILAIQGDRVETFNDVVRTVSTSPGRELAVEVKRGDDRLTLHVTPERQ
                     EVKDNFGGTIVRGIIGIRRVTTPETLELRRAGPLEAIWLGVNETRFIITSTLSYIGDV
                     IIGRQSPDQLGGPIRIAEVSGQVAAIGPEALLNLIAVISVSIGLINLFPIPLLDGGHL
                     LFYAIEALRRKPLSERTQEIGFRIGLAVVLMLMVFATWNDRLIVWRWITGNG"
     gene            complement(3666..4826)
                     /locus_tag="VF226_13005"
     CDS             complement(3666..4826)
                     /locus_tag="VF226_13005"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX7074952.1"
                     /translation="MKTVSILGATGSVGASTLDIIGQATDAFEVVAVTAQTNAAKLAA
                     VARQHRAKLAVIGDAAHYAELRDLLSGTGIDVAAGPAALVAAAEQPADCVVAAITGAA
                     GLAPTLAAARQGRRLALANKECLVAAGDIFMATVNRSKTELVPVDSEHSGVFQLLSGA
                     DAKAVERIVLTASGGPFREWRMDRLKTATPEEAVKHPKWSMGPKISVDSATLMNKGLE
                     LIEAYHLFPVTAGQIAVVVHPQSVVHSFVAFQDGSVMAQMSCPDMRIPISVGLAWPAR
                     MRFPAERLDLVRLHALTFEAPDLERFPALEIARGALERGGTAPAILNAANEVAVESFL
                     ARRLGFLDIARTVATCLDRAEQAGLIQTPQSLDDVLAADAAGRRLARDVIAG"
     gene            complement(4928..5794)
                     /locus_tag="VF226_13010"
     CDS             complement(4928..5794)
                     /locus_tag="VF226_13010"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7074953.1"
                     /translation="MSGNQHVEGAARSAGLGHDLKPRIISGLLLAALALGLTYAGSVT
                     FAILVIPIALIMCWEWGRVVRGEQLDAAFAIHAGAVALATLLAAVGLAALGLLLLLIG
                     AILVLLLQFNVGGRLSALGVPYVGIPAIALLWIRADEPYGFAAVLFLLLVVCATDTFA
                     YFGGRSIGGARLWPRISPNKTWAGFVSGIAAGTLVGALYPFFVAGASHLGLALTAFLL
                     AIVSQAGDLAESALKRGSGVKDASMLIPGHGGFLDRMDGLVFAATAAALLCLYANLQS
                     PAHALLFGLQLP"
     gene            complement(5791..6561)
                     /locus_tag="VF226_13015"
     CDS             complement(5791..6561)
                     /locus_tag="VF226_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX7074954.1"
                     /translation="MQVRPAADTDEGVPPLRHVAIIMDGNGRWANRRGLPRSVGHRMG
                     VEALRRTVRAAIELSIPYLTLYTFSSENWSRPASEVDELMRMMKRFVRKDLVDLHQNG
                     VRIQVIGERSNIDPELLGLIEEAETVTAANERLSLIVAFNYGSRVEIAKAARRLADKA
                     ARGLLDPAEITPEVLASELDTCGIPDPDLVIRTSGEVRLSNFLLWQAAYAELIFIDAC
                     WPDFGRELLEQAIAEYRSRDRRYGGLSKRANGLEVRRL"
     gene            complement(6633..7196)
                     /gene="frr"
                     /locus_tag="VF226_13020"
     CDS             complement(6633..7196)
                     /gene="frr"
                     /locus_tag="VF226_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7074955.1"
                     /translation="MAQGNFDIGDLERRMRAAIEALKRELSGLRSGRASANLLDPVMV
                     NVYGQKMPLNQVGSVSVPEPRMITVHVWDKAAVPAVDKAIREANLGLNPIIDGTILRL
                     PIPPLTQERRQELIKLAHKYAEQSRVAIRNVRREGMELLKKLEKDSKMSQDEQRTNST
                     KVQELTDKLIKEVDQILANKEAEINKV"
     gene            complement(7280..8011)
                     /gene="pyrH"
                     /locus_tag="VF226_13025"
     CDS             complement(7280..8011)
                     /gene="pyrH"
                     /locus_tag="VF226_13025"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7074956.1"
                     /translation="MSEAGPLRYRRLCLKLSGEAMSGKGEFGIDMAMVTRLARDVGEA
                     VEAGVRIVVVLGGGNIFRGLAGAAKGMDRATADYMGMLATVMNALAFHNALEQIGVGA
                     RVLSAIPMPTICESYVRAKALHHLEHRRVVICAAGTGNPFFTTDTTAALRAIEMNCDA
                     VAKATQVDGVYSADPRTNPDAVRYDRLTYADVLARDLRVMDGAAIALARDNGLPVIVF
                     SIEESGNLLKMLRGEARATVIQGSV"
     gene            complement(8246..11734)
                     /gene="mfd"
                     /locus_tag="VF226_13030"
     CDS             complement(8246..11734)
                     /gene="mfd"
                     /locus_tag="VF226_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEX7074957.1"
                     /translation="MDTLVQHDLILSGVPEGVDALVLARLTREAAAASGGPGFVLHVA
                     RDDRRLEALRGALAFFDPKVRVIAFPAWDTVPYDRVGPNAEIVAKRMTALARLVVGGR
                     REPTLILTTVNAILQRVPPRQFVRNAIRQMAPGQRIDMNRLVQRLLLGGYQRTGTVME
                     PGEFAVRGGILDMFPPGRANPVRLDFFGDTLESIKTFDPETQRTHKLVQKLTLLPVSE
                     VAFGEEAERTFRRRYVELFGGGTADDPLYQAISAGQRYPGMEQWLPLFHDKLETLFDY
                     VQGSDVSFDHLADDVVSQRFEQVMEHYQARVESLETAKFGAPPYKPVPPELMYISGSE
                     WRSYLGALTVRRFTPFEQDERTHSASLGGRPGRNFAPERTAESSDLFGAVVGHVRRLI
                     SDNKRVVVAAWTPGARERLASLLSAHGLSEIRKVDSYTEVLSLPGSVVGLAVLGLEQG
                     FETPDLAVIAEQDILGDRLVRPRRKAKKAADVITEATSLSVGDLVVHVDHGIGRFEGL
                     QTITALGAPQDCLELVYAGGDKLYLPVENIELLSRYGSDAEGVQLDKLGGVAWQSRKA
                     RLKKRLREIASELIKIAAMRQLKEAPALAPPAGAYEEFVARFPYEETEDQLASIEAVL
                     EDLNTGRPMDRLVCGDVGFGKTEVALRAAFVTAMAGLQVAVVVPTTLLARQHTQTFID
                     RFKGLPVRIAQASRLVPAKELAEVKEGLRNGTIDIVIGTHALLGKSIKFARLGLLIVD
                     EEQHFGVAHKERLKQLREDVHVLTLSATPIPRTLQLALSGVRELSLITTPPVDRLAVR
                     TYISPFDPVIIREALRRERYRGGQAFYVVPRISDLDDVAEFLREAVPELRVARAHGQL
                     SVTELEDVMTAFYEGEYDVLLSTAIVESGLDVPNANTLIVHRADMFGLAALYQLRGRV
                     GRSKTRAYAYFTTPPGQKLTEGAEKRLKVLHSLDTLGAGFSLASHDLDIRGAGNLLGE
                     EQSGHIREVGFELYQSMLEEAVATLKGGDLGEAAEQWSPQISLGTSVLIPETYVADLQ
                     LRLGLYRRLATLETRADIDAFAAELVDRFGELPQEVRHLLDVMEIKGLCRQAGIAQVD
                     AGPKGAVLSFRKNTFANPEGLVDLVQRSRGKMKLQPDHKLVLKADWDRPDKRLNGVRQ
                     LISDLAKLTAKRTEVK"
     gene            complement(11882..12148)
                     /locus_tag="VF226_13035"
     CDS             complement(11882..12148)
                     /locus_tag="VF226_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX7074958.1"
                     /translation="MRGDDLDTRRRRAAYRATHRGTKEMDWIIGRYATEVLPGLEGEA
                     LERFERLLSLPDPELQKWLLDPQLAGASEFAELITQMRRFHRLE"
     gene            12339..14453
                     /gene="recG"
                     /locus_tag="VF226_13040"
     CDS             12339..14453
                     /gene="recG"
                     /locus_tag="VF226_13040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7074959.1"
                     /translation="MRPSVLTPLFASAQSLAGVGPRVLVLLKKALRLPPGVTEPRVID
                     LIWHTPTGVIDRRAEPTVADAVPGTIATLAVRVLKHKGPPRGNAKAPYKVTCEDDTGR
                     IDLVFFHAERRFIEKQLPIGSIRYISGRVERYGDTLQMAHPDYIVAPEARADLPMLEP
                     VHPLTAGLSGKILLKLMRQAVERVPPLPEWQEPRWLAARRWPAFHDAVTRLHVPRETD
                     DISPGAPNWQRLAYDELLAGQVALALVRQRLKNQPGRAVKGDGRIRRELMAALPFALT
                     SSQVKALREIEADMAAPQRMLRLLQGDVGSGKTAVALMSMAIAVEARAQAALMAPTEV
                     LARQHVETIAPLADKVGLRIGLLTGREKGKPRAEVLDRLARGEIDILVGTHALFQPDV
                     VFKDLAFAVIDEQHRFGVHQRLALQTKGGHGVADVLVMTATPIPRTLLMTHYGDLDVS
                     RLTEKPAGRKPVKTTLASVERLEEVVDGLRRAIAEGAQVYWVCPLIEGNATTELAAAE
                     ERHAHLTQVLGAEVGLLHGALPGRVKDATMEAFAEGRLKVLVSTTVIEVGVNVPNATI
                     MVIEHAERFGLAQLHQLRGRVGRGDRQSYCVLLYKPPLGETAEERLKMMRETEDGFLI
                     AEKDLELRGGGEVLGARQSGMPEFRIANVPNFEELLAAARDDARLLLHEDPSLTSPRG
                     AAIRTLLYLFECDDAVRLFRAA"
     gene            complement(14513..15292)
                     /locus_tag="VF226_13045"
     CDS             complement(14513..15292)
                     /locus_tag="VF226_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7074960.1"
                     /translation="MQNNGTEQQPLPGANPSAQAKPPRPGVVQTHSELHLGARLRNYF
                     LTGLVVVGPVTITLYIAWWFINVVDAWVKPFVPRIYNPETYLPFPMPGIGLLFGIVGL
                     TLIGALAANLLGRTIISSGELFLQRMPIVRNLYQALKQIFVSVVSAAGPGTSFNKVGL
                     MEFPSKGIWSLVYITGEVAGELKEIRPGGEDDLLTVFMPTGIMPPTGFVCLVPRRDVI
                     LLNMSVEDAAKMVISAGMVMPEYQKQLKTLADASRGEEVKP"
BASE COUNT         2802 a         5144 c         4899 g         2621 t
ORIGIN      
        1 ctgaggaacg gatgctggcg tcgtcagcaa ggcaaacgcg acgaacgttc ggatcagcat
       61 tattgcattc tgcatccaac tccttagctc tcgcacccgc tccgaacagc gagccagcat
      121 ccgcgaaaac agcacccgac atgccgagct catctggtat gagcggcaat gggaaacgga
      181 cctctgccgt cgctgcccag taggtcttgc cacccagcgc atcaccggta tcaatgtccc
      241 gcgggccata accagcccga tcgaaaccgc ggacggtttc accgcctctg taatagaggt
      301 ccagcagacg tacgtcttgt ccgccccacc cctcgatatg gccgccaatc acacggccga
      361 cgaaggtgat ttcatcggtg atggggtagt aagcccgccc ctcaccttgc acccgaacgt
      421 actgcacatc gccgcccacg cccgcaaagt ccgaaccaac ctggaagtaa agaccccggt
      481 ttgggttacg cgggtgattg cgagtatcgt aggtgattgc ggcgccgagg gcagaggtca
      541 gtgccgcacc ttcagcctcc ttcactacct cagacgcccg atcatccaca tcaaaaatat
      601 cgtcatgcga cagggtatag cttgttgaca tccagaagtt ctcagccaac gggaagccca
      661 tacgcaggct gccaccggta cgacgactct tgaacgatga ctgatcggac tgatcgatct
      721 ccttgtggaa gaggtcgaaa ccagcagcga tattccgatc aaggaagcgt ggctcggtga
      781 agctcagatc gatctggaaa cgctccacgg agccgcccaa ggccaagcgt aggaattggc
      841 cgttacccaa taggttgcgc tcggtgatgc tgacatcgcc gatcacaccc tcgttggtcg
      901 agtagccggc gccgaaggac agctcacccg tgggctgctc ggtaacctcg acatcgagca
      961 ccacccggtc cggggcagag cccgggcgcc gctggatctc cacggccttg aagaatccca
     1021 acgcctgcag gcgcttcttg gcctgatcga tcagcagcgg attgtaggcg tcgccctccg
     1081 ccagccggaa ttcgcgtcgg atgacacggt ccttggtgcg caagttgccg acgacattga
     1141 tgcgctcgat atacacgcgt ggaccctggt cgatctcata ggtgatatcg atcgaccggg
     1201 ctgcgggatt gcgatcggca cgaggacgaa cacgcgcaaa ggcgaatccc agctcggagg
     1261 tgcgcagggt cagcttctcg accgtcttgt cgatgtagga cgcattgaag gtctccccgg
     1321 gccgggtcag cagctcgtcg ttgagcgtct cggggtgcag ctcgggcaac tgactgtcga
     1381 tgcgaacgtc gccgaatacg tacggctggc cttcctcgat gacgaaagtc aggatgaaac
     1441 ccgttccgtc gcggtcgagc tctgcgtttg ccgagatgat gttgacgtcg gcgtagccgt
     1501 tcttgaggta gtactggcgc agcagctcgc ggtcgaggtt catgcgatcg ggatcataga
     1561 tcgccgtgcc cttgaggaaa tcgaaccagc cggactgggt cgtcgtgatg atgtcgcgaa
     1621 gctgcgaatc cgagaaggcg tgattgccga cgaagtggat cgccttgacc ttcgtcgcta
     1681 tgccctcgtt gatctcgaag acgacattca cgcggttttc gtcgagctcg atgagatttg
     1741 gctcgacaac cgcagcaaac cgcccctgcc gccgataaac gtcgagaatg cgctgcgcat
     1801 ccgccaacgc gcgggcgcgc gtgtagaccg accgcggctt gagctgcacc tcggcctcga
     1861 gcgtcttgtc gtcgatctcg ctgttgcctt cgaaggcgac ctggttgacg accggattct
     1921 cgacgacgtg cacgatcacc gtgctgttct gccgctcgat ctctacgtcc gcgaagaggc
     1981 ccgtggcgaa caacgctctc agcgagctgt ccgccttacc tgcatcgtaa gggtcgccta
     2041 ccgtgaaatg gaggtaggaa cggaccgttt cgggctcgac gcgacggttc ccggtgacct
     2101 tgatctcgcg cacgatcacg ccctgagcct ggacagccgg cacagctgcg aagctggtca
     2161 gagcggaggc ggaaacactg atggcggcga ccgaaagaca aagccgccac ccccccatta
     2221 gccatttccg aagtcgcata cctgcgtctg cccttcgtta tcggtgcccc ccggaagcgg
     2281 cgttgatcgc ctcacagcgg acaacgcaac tccatattag ctgagtcgcc agggtggctc
     2341 taaccggcca tttcgcctcg ggttagactc agccaacggt caggttttaa cgagttggca
     2401 ggggcattgg aagtggcaag aatgtttcac aaccgcgtaa aatgctttgt ggactaacca
     2461 tttccggtaa tccagcgcca cacgatcagg cgatcgttcc aggtcgcgaa caccatcagc
     2521 atcagcacca cggcaagccc gatccggaac ccgatctcct gcgttcgctc gctcagcggc
     2581 ttccggcgca aggcctcgat ggcatagaac aaaaggtgac cgccgtcgag cagcgggatg
     2641 gggaacaggt tgatcagacc gatgctgacg gaaatgacgg cgatcaggtt gagcagggct
     2701 tccggaccga tcgcagccac ttggccggac acctccgcga tgcggatcgg gccgccgagc
     2761 tgatcggggc tctggcgtcc gatgatcacg tcgccgatgt aggacaacgt cgaggtgatg
     2821 atgaatctcg tctcgttgac tccgagccag atcgcctcga gcggtccggc gcgcctcagc
     2881 tcgagcgttt caggggtcgt cacccgcctg atgccgatga tgccgcggac gatggtgccg
     2941 ccgaaattgt ccttaacctc ctggcgctcg ggcgtcacgt ggagcgtcag cctgtcgtcg
     3001 ccccgcttca cctcgaccgc gagctcgcgc cccggcgagg tgctgaccgt ccgcaccacg
     3061 tcgttgaagg tctccacgcg gtcgccctgg atggccagga tcacgtctcc cggcaagaag
     3121 ccggccacgg ctgccggtga gttcggcacc acctcgtcga cccgcggcgc cgtcgagcgc
     3181 acgccgatca gcgagaacat cagggcaaaa ataacgattg ccagcaggaa gttagcgatc
     3241 gggccggccg cgacgacggc agcccgttgc cagagcggct tggcatggaa cgcgccggcg
     3301 cgctcctccg gcgtcatctc ctcgagtctg gccgatggtc ccgcactcgc cgcgttctcg
     3361 tcgtccatga acttgacgta gccgccgagc ggaatccacg agatccgcca tctggtgccg
     3421 tgtcgatcga agaagccgaa tatttcccgc ccgaacccga tcgagaaggc cttgatgctg
     3481 acaccgcacc agcgcgccac caggaagtgt cccaactcgt gcacgaacac gacgacggtc
     3541 agcacgaaca ggaacgacac caggacgaac agaaatcctc cgatcccgtc ggcgaagctg
     3601 gacagcagtg ccatcaactc ggtctcctga actcttggcg cagcaccggg cgcatcaacc
     3661 cccgcttacc ccgctatcac atcccgtgcc aagcggcggc cggcggcatc ggcggccagg
     3721 acatcatcaa gactttgcgg cgtttggatg aggccggcct gctccgcccg atcgaggcat
     3781 gtggcaaccg tgcgggcaat gtcaagaaag cccaatcgcc gcgcaagaaa gctttcgacc
     3841 gcgacctcgt tggcggcatt gagaatggcg ggcgccgtgc cgccccgctc cagcgccccg
     3901 cgggcgatct cgagagcggg aaagcgctcc agatcaggcg cctcgaatgt cagcgcatga
     3961 agccgcacga gatcgagccg ctccgccggg aaccgcatcc gcgcgggcca cgcgagcccc
     4021 acggatatcg gaatgcgcat gtcaggacag gacatctggg ccatgactga cccgtcctga
     4081 aacgcaacga aactatgcac caccgattgc gggtgcacaa caacggcgat ctggccggcc
     4141 gtgaccggga acaggtggta ggcctcgatc agctctagcc ccttgttcat gagcgtcgcc
     4201 gagtcgacgg agatcttcgg ccccatgctc cacttgggat gcttcaccgc ctcctcgggc
     4261 gtcgcggtct tgagccgatc catcctccat tcgcggaacg gcccgccgga tgcggtcagc
     4321 acgatccgct ccaccgcctt ggcgtctgct ccgctcagga gctggaacac gccggagtgc
     4381 tccgagtcga ccggcaccag ctcggtcttt gagcggttca cggtcgccat gaagatgtcc
     4441 ccagcggcga cgaggcattc cttgttggca agagccaggc ggcgcccttg ccgggccgcg
     4501 gcgagcgtcg gcgcgagccc cgccgcaccg gtgatggcgg ccacgacgca gtcggccggc
     4561 tgttctgccg cggcaacgag agccgcgggg cccgccgcca cgtcgatacc ggtccccgac
     4621 agcagatcgc gaagctcggc gtagtgcgcc gcatcaccga tcaccgccag cttcgcacga
     4681 tgctggcgcg cgacggcggc gagcttggcg gcgttcgtct gagcagtgac tgcaacgacc
     4741 tcgaacgcat cggtcgcctg cccgatgata tcgagtgtgc tcgccccgac ggatcccgtg
     4801 gcccccagaa tactgaccgt cttcacctgc cttcgaccgc ctcagaattg ctgattgaca
     4861 agatcagacc gccggcggcc tcgcggccgc cgcaggctcg atgttttcga tctcgcagtg
     4921 actggtgtca cgggagctgc agcccgaaca gcagcgcatg ggcgggcgac tgcaaattgg
     4981 catagaggca gagcaatgcc gccgccgtgg ccgcgaagac gaggccgtcc atacggtcga
     5041 gaaagccgcc atgaccagga atgagcatgc tcgcgtcctt gacgccggac ccgcgcttca
     5101 gcgccgactc ggcaagatcc cctgcctgcg aaacgatcgc gaggaggaaa gccgtgagcg
     5161 ccaggcccag atgcgaagcg cctgcgacga agaagggata gagagcaccg acgagcgttc
     5221 cggccgcaat gccgctgacg aaaccggccc aggtcttgtt cggcgatatg cgtggccaca
     5281 gacgggcgcc tccgatgctg cgccccccga agtaggcaaa cgtatcggtt gcgcacacca
     5341 cgagcaagag gaacaggacc gcggcaaatc cgtatggttc atcggcccga atccagagca
     5401 gcgcgatcgc cggaatgccg acgtagggaa cgccgagcgc cgacaatcgc cctcctacgt
     5461 tgaactgcag gagcagcacg aggatggcgc cgatcagcag caggaggagc cccagcgcgg
     5521 caagcccgac ggcggccagc aacgtggcga gcgcgaccgc gccggcgtgt atggcgaacg
     5581 ccgcatcgag ctgctcgccc cggaccacgc gcccccactc ccagcacatg atcagggcaa
     5641 tcgggatcac gaggatggcg aaggtgacgg acccggcata ggtaagcccg agcgccaggg
     5701 ccgcgagcaa tagtcctgag atgatgcggg gtttcagatc atgaccgagc cccgcggatc
     5761 gcgcagcccc ctccacgtgt tggtttccgc tcatagccgg cgcacttcca ggccgttggc
     5821 gcgtttcgac aggccgccat accgccggtc ccgtgaacgg tattcggcga tcgcctgctc
     5881 gagcagctcg cgcccgaaat cgggccagca ggcgtcgatg aagatcagct ccgcgtaagc
     5941 ggcctgccat aaaagaaagt tcgacaagcg gacttcgccg ctggtgcgga tgacgagatc
     6001 cggatcgggt atgccgcagg tatcgagttc tgaggcgagc acttcgggcg taatctcggc
     6061 cgggtcgagc agaccacgtg ctgccttgtc ggcaaggcgg cgggcagcct tggcgatctc
     6121 cacccgagag ccgtagttga aagcgacgat gagcgagagc cgctcgttgg ccgcagtgac
     6181 cgtctccgcc tcctcgatca gacccagcaa ctccggatcg atgttgctgc gctccccgat
     6241 cacctggatg cgcacgccgt tctggtgcag gtcgacgagg tccttgcgca cgaagcgctt
     6301 catcatgcgc atcagctcgt cgacctctga cgcgggccgc gaccagttct ccgatgagaa
     6361 ggtgtagagc gtcaaatagg ggatcgagag ctcgatagcg gctctcacgg tgcggcgcag
     6421 agcttccacg cccatgcgat ggcccacgga ccgaggcagg ccgcgccggt tcgcccagcg
     6481 accgttgcca tccatgatga tcgccacatg acgcagcggc gggacgcctt catccgtgtc
     6541 cgcggctggc cttacctgaa tatctgccac cgagccagta agctttctct ttgaaattgc
     6601 tcaggcggag gcacccgccc cccttttgag tatcagacct tgttgatctc ggcttccttg
     6661 ttcgccagga tctgatccac ttccttgatg agcttgtctg tcagctcctg caccttcgtc
     6721 gagttggtcc gctgctcgtc ttggctcatt ttcgagtcct tttcgagctt cttcagcagc
     6781 tccatgccct cacggcgcac attgcgaatg gcgacccggc tctgctccgc gtacttgtgc
     6841 gccagcttta tgagctcctg acggcgctcc tgcgtcaacg gaggaatggg caagcgcagg
     6901 atggtgccgt cgatgatcgg attcaagccg agattagcct cgcggatcgc cttgtcgacg
     6961 gccggcaccg ccgccttgtc ccaaacgtgg accgtaatca tgcggggctc cggcaccgag
     7021 acggagccga cctggttcag cggcatcttc tgcccgtaca cgttgaccat gacggggtcg
     7081 agcagattgg cgctcgcacg acccgacctc agcccgctca attcccgctt gagcgcttcg
     7141 atcgcggcgc gcatgcgccg ttccagatcg ccgatatcga agttgccctg agccatacca
     7201 ttcctcctgc cgggcgcggc ggtggcccgg cgcttgtcgt caggcggcga aaggcgcggc
     7261 ggggccgcgc gatctcgaat caaacgctac cctgaataac ggtcgctcgg gcctcgccac
     7321 gcagcatctt caaaagatta cccgactcct cgatcgagaa aacaattacc ggaagaccat
     7381 tgtcgcgggc aagcgcgatg gcggctccgt ccatgacccg gagatcgcgc gccagcacgt
     7441 cggcatacgt gagccgatcg tagcggacgg catccggatt ggtccgcggg tccgccgaat
     7501 agacgccgtc gacctgcgtt gccttggcga ccgcatcgca gttcatctcg atggccctga
     7561 gcgcggcggt cgtatcggtc gtgaagaacg ggttgcccgt gccggcggcg catatgacaa
     7621 cgcgccggtg ctcgagatga tgcagggcct tcgcgcgcac gtaggattca cagatggtcg
     7681 gcatcggaat ggcggagagc acgcgcgccc caactccgat ctgctccagg gcattgtgga
     7741 aggcgagcgc gttcatgacg gtcgcaagca tgcccatgta gtcggccgtg gcacggtcca
     7801 tgcccttggc ggcgccggcg agcccgcgga agatgttgcc gccgcccagc accacgacga
     7861 tccgcacccc ggcctcgacg gcttcgccaa cgtccctggc gaggcgggtg accatggcca
     7921 tgtcgatgcc gaactcaccc ttgccgctca tggcctcgcc cgacagtttc aggcacaggc
     7981 gcctatatcg cagtgggccc gcttcgctca tccttctcgc ctccttaccg ccgtcaggtc
     8041 cgccatcccc ccgcaggggg cggtcaactc tcaggtgccg cgtccattcg cgtgcgcttg
     8101 tgctcgcgcc aagaactcag gttgcaaccc gcaaacgcgc ggcgccacgg atcgattaag
     8161 ccttcgctgc gctggcaccg gtctggtctg ccctggggcg ccgaccgcat agcccgtcag
     8221 ccgaccacgt acaagtggtc aggcctcact tcacctcggt gcgtttcgcg gtcagcttcg
     8281 ccaaatccga gatcagctgc cgtacaccgt tcaagcgctt gtccgggcgg tcccaatccg
     8341 ccttgagcac gagcttgtga tccggctgca gcttcatttt gccccgagag cgctgcacca
     8401 ggtcgacgag cccttccggg ttggcgaacg tgttcttccg gaaagacaga acggccccct
     8461 tcggcccggc atcgacctga gcgatgccgg cctgacggca gaggcccttg atctccatca
     8521 cgtcgagcag gtggcgcacc tcctggggca gctcgccaaa gcgatcgacg agctcggccg
     8581 cgaacgcgtc aatgtcggcg cgcgtctcga gcgtggcgag ccggcggtaa aggccgagcc
     8641 tgagttgcag gtcggcgacg taggtctcgg ggatcagcac cgaggtgccg agcgagatct
     8701 gcgggctcca ctgctctgcc gcctcgccga ggtcgccgcc tttgagcgtc gcaaccgcct
     8761 cctccagcat cgactggtag agctcgaagc cgacctcgcg gatatggccc gactgctcct
     8821 cgccgagcag gttccctgcg ccacggatgt cgaggtcgtg gctcgcgagc gagaagcctg
     8881 cccccagcgt atcgagcgaa tgcagcacct tgagccgctt ttccgcgcct tccgtgagct
     8941 tctggcccgg aggcgtggtg aagtaggcat aggcacgggt cttggaccgc cccacacggc
     9001 cgcgcagctg gtagagcgcc gccaacccga acatatccgc ccggtggacg atcagggtgt
     9061 tggcgttggg cacgtcgagg cccgactcaa cgatggccgt cgacaggaga acgtcgtact
     9121 cgccctcgta gaacgcggtc atcacgtcct cgagctcggt gaccgagagc tggccatggg
     9181 cacgggcgac ccgcagctcc ggaacagcct cgcgcaggaa ctcggcgacg tcgtcgagat
     9241 cggaaatgcg cggaacgacg tagaacgcct gcccgccgcg gtagcgctcc cggcgcagcg
     9301 cctcccggat gatgaccggg tcgaacggcg agatatacgt gcgcaccgcc agccggtcga
     9361 ccggcggcgt ggtgatgagc gacagctcgc ggacgccaga gagggcgagc tgaagcgtgc
     9421 gggggatcgg cgtcgccgaa agcgtcagca cgtgaacgtc ctcgcggagc tgcttcaggc
     9481 gctccttgtg cgcgacgccg aaatgctgct cctcgtcgac gatcagcagg ccgaggcggg
     9541 cgaacttgat ggacttgccg agcagcgcat gcgtgccgat gacgatgtcg atcgtaccgt
     9601 tgcgcaggcc ctccttgacc tccgccagct ccttcgcggg aaccagccgc gaggcttggg
     9661 cgatgcggac gggaagtccc ttgaacctgt cgatgaatgt ttgggtgtgc tggcgggcga
     9721 gcagtgtcgt cggcaccacc accgccacct gcaggccggc catggcggtg acgaacgccg
     9781 cgcgaagcgc cacctccgtt ttgccgaacc cgacatcgcc gcagacgagc cggtccatgg
     9841 gccggccggt attcaggtcc tcgaggaccg cttcgatgct cgcgagctgg tcctccgtct
     9901 cctcgtaagg gaatcgggcg acgaactcct cgtaggcccc cgccgggggc gcgagcgcag
     9961 gcgcctcctt gagctggcgc atggcggcga tcttgatgag ctcggaggcg atttccctca
    10021 ggcgcttctt gagccgggcc ttgcgcgatt gccaggcgac gccgcccagc ttgtcgagct
    10081 gcacgccctc cgcgtcggag ccgtagcgcg atagaagctc gatgttttcg accggcaggt
    10141 agagcttgtc accgcccgca tagacgagct cgaggcagtc ctgcggggcg ccaagggcgg
    10201 taatcgtctg caggccctcg aaccggccga tgccgtgatc gacgtggacg acgagatcgc
    10261 cgacggacag gctcgttgcc tcggtgatga catcggccgc cttcttggcc ttgcggcgcg
    10321 gtcggacgag ccggtcgccg aggatgtcct gctcggcgat cacagcgagg tcgggcgtct
    10381 cgaagccttg ctcgagaccg agcacggcca gccccaccac acttcccggc agcgaaagca
    10441 cttccgtata gctgtcgacc ttgcggatct cgctcagtcc gtgggccgac agcagcgaag
    10501 ccaatcgttc gcgcgcgccg ggtgtccatg ccgctacgac gacacgcttg ttgtccgaaa
    10561 tcagccgccg gacatgtccc acgaccgcac cgaacaggtc gctactctct gctgtccgct
    10621 ccggcgcaaa gttgcggccc ggccggccgc cgagactggc cgaatgggtg cgctcatcct
    10681 gctcgaaggg cgtgaagcgg cgcaccgtca gcgcaccgag gtaggagcgc cattcgctgc
    10741 ccgaaatata catcagctcg ggcggaacgg gtttgtaggg cggagcgccg aacttcgccg
    10801 tctcgagcga ctcgacccgc gcctgatagt gctccatcac ctgctcgaac cgctgggaga
    10861 cgacatcgtc ggcgaggtga tcgaagctca cgtcgctgcc ctggacatag tcgaacagcg
    10921 tttccagctt gtcgtggaac agcggcagcc actgctccat gcccggatag cgttggccgg
    10981 cgctgatcgc ctgatagagc gggtcatccg ccgtgccccc gccgaacagc tcgacatagc
    11041 gcctgcgaaa tgtcctttcg gcctcctcgc cgaaggccac ctcgctcacc ggcagaagcg
    11101 tgagcttctg gacgagcttg tgcgtgcgct gcgtctcggg atcgaaggtc ttgatgctct
    11161 ccagcgtatc gccgaagaaa tcgagccgca ccgggttcgc gcggcctggc ggaaacatgt
    11221 cgagaatgcc gccgcgaacg gcgaactcgc cgggctccat caccgtgccc gtgcgctggt
    11281 agccgcccag cagcagccgc tgcacgaggc ggttcatgtc gatacgctgg ccgggcgcca
    11341 tctggcggat ggcattgcgc acgaactggc gcggcggcac gcgctgcagg atggcgttga
    11401 cggtggtgag gatcagcgtc ggctcgcgcc ggccgccgac caccagccgg gccagtgccg
    11461 tcatccgctt ggcgacgatc tccgcattgg gtccgacgcg atcataagga acggtatccc
    11521 aggccgggaa agcgatgacg cgaaccttcg gatcgaagaa ggcgagcgcc ccgcgcagcg
    11581 cctcgagccg ccggtcgtca cgcgccacgt ggaggacgaa ccctggcccg ccgctcgctg
    11641 cagccgcctc ccgcgtcagc cgcgcgagga ccaaggcatc gacgccctcg gggacgccgg
    11701 aaaggattag atcgtgttgt accagcgtat ccatgtgccc gtcgccggcc cgatggccgt
    11761 cgaacaattc gaatttcaaa tcgtcatccc ggtgcttgtc accgggatcc atctgtccgc
    11821 tcgctccagc gcctgtcgtt ggccggatcc cgggcataag gcccgggata actgatgatt
    11881 gctactccag ccggtgaaac cggcgcatct gcgtgatcag ttctgcgaac tcgctggctc
    11941 cggcaagctg tggatcaaga agccacttct gcaactcggg atcgggcagg ctcagcagcc
    12001 gctcgaaacg ctccagcgcc tcgccctcta gtccaggcag cacttcggta gcgtaacggc
    12061 cgatgatcca gtccatttcc ttggtgccgc ggtgagttgc gcgatacgcg gctcttcgcc
    12121 ggcgcgtgtc gagatcgtcg ccccgcatag cctttccctc gccgcttctg gatatcctgc
    12181 cgggcgcctt gacgctcccg ccccgcgccg ccaacgtgca ttgctaaagg cgtgatatag
    12241 cgcccgcagt cccgcacgca aagggtgagc cgcgacgcac caacaggcag cccccggagc
    12301 ggcggcaaca ggcttcgatc atcggcaacc tgaacgcaat gcggcccagc gtgctgacac
    12361 ccctcttcgc ctcggcgcag tcgctggccg gtgtcgggcc acgcgtcctg gtgctgctga
    12421 agaaggccct gcgcctgccg cccggcgtca ccgagccgcg ggtcatcgac ctcatctggc
    12481 ataccccgac tggcgtcatc gatcgccggg ccgagccgac ggttgccgat gccgtgccgg
    12541 gcacaatcgc gacccttgcc gtgcgcgtgc tgaagcacaa ggggccgccg cgtgggaatg
    12601 ccaaagcgcc ctacaaggtc acatgcgagg acgacaccgg gcgcatcgac ctcgtcttct
    12661 tccacgccga gcggcggttc atcgagaaac agctccccat cggcagcatc cggtacattt
    12721 caggccgcgt cgagcgatac ggcgatacgc tgcagatggc gcacccggat tacatcgtgg
    12781 cgccggaggc ccgcgccgac ctgcccatgc tggagccggt ccatccgctg acggcaggtc
    12841 tgtccggcaa gatcctgctc aagctgatgc gtcaggcggt cgagcgggtc ccgccgctgc
    12901 ccgagtggca ggagccgcgc tggctcgccg cgcgccgctg gcctgccttt cacgacgccg
    12961 tcacgcggct gcatgtgccg cgcgaaaccg acgacatctc tcctggcgca ccgaactggc
    13021 agcgcctcgc ctacgacgag ctgctggcgg gccaggtggc gttggccctg gttcggcagc
    13081 gcctcaagaa tcagcccggc cgcgctgtta agggcgacgg gcgcatccgc cgggagctca
    13141 tggctgccct gcccttcgcg ctgacatcct cacaggtcaa agcgctaaga gagatcgagg
    13201 cggacatggc ggcgccgcag cggatgcttc gcctgcttca gggcgacgtc ggctccggca
    13261 agactgcggt tgcgctcatg agcatggcga ttgccgtgga ggccagagcg caggcagctc
    13321 tcatggcacc cacagaggtg ctggcgcgcc agcacgtgga gacgatcgcg ccattggcgg
    13381 acaaggtcgg gttgcgcatc ggcctactga cgggccgtga gaaaggcaag ccgcgtgctg
    13441 aggttctcga ccggctggcc cgaggcgaga tcgatattct cgtcggcaca catgccctgt
    13501 tccagccgga tgtggtgttc aaggatctgg cctttgccgt gatcgacgag cagcaccggt
    13561 tcggcgtgca tcagcggctc gcactgcaga cgaagggcgg ccatggcgtc gccgatgttc
    13621 ttgtgatgac ggcgacaccc attccccgca cgctgctgat gacgcactac ggcgacctcg
    13681 acgtctcacg gctcacggag aaacccgcag gccggaagcc ggtgaagacg acgctggcct
    13741 cggtcgagcg gctggaggag gtcgtcgacg gcttgcgccg cgccattgcc gaaggcgcac
    13801 aagtctactg ggtctgcccc ctgatcgaag ggaatgccac gacggagctc gcggccgccg
    13861 aggagcgcca cgcgcacctc acccaagtgc tcggtgccga ggtgggcctg ctgcacggag
    13921 cgttgcccgg ccgcgtgaag gacgcgacca tggaggcgtt cgccgagggc aggctgaagg
    13981 tgctcgtgtc gacgacggtg atcgaggtcg gcgtcaacgt gcccaatgcc accatcatgg
    14041 tcatcgagca tgctgagcgc ttcggcctcg cccagcttca ccagctccgc ggccgcgtcg
    14101 gacgtggcga tcgccaatcc tactgcgtgc tgctctacaa gccaccgctt ggcgagacgg
    14161 cggaggagcg cctcaagatg atgcgcgaga ccgaggacgg cttcctcatt gccgagaagg
    14221 atctggagct gcggggcggc ggcgaggtgc tgggcgcccg ccagagcggc atgccggagt
    14281 ttcgcatcgc caacgtgccg aacttcgagg agctcctggc cgccgcccgt gacgacgccc
    14341 ggctgctgct tcacgaggac ccgtcgctca ccagcccgcg cggggcggct atccggacgc
    14401 tgctctatct cttcgagtgc gacgatgcgg tgcggctgtt ccgggcggcg tgacgctcgt
    14461 gagtttgtga tcagaccgat gctcgaagtc tcctcctcac gagctcacaa tatcacggct
    14521 tgacctcctc gcccctgcta gcatccgcca acgtcttgag ctgcttctga tattccggca
    14581 tcaccatgcc ggccgagatg accatcttgg cggcgtcctc gacgctcatg ttgagcagga
    14641 tcacgtcccg gcgcggcacg aggcaaacga agccggtggg gggcatgatc ccggtcggca
    14701 tgaacacggt cagcaagtcg tcctcgccgc cgggccggat ctccttcagc tcgcccgcca
    14761 cttcccccgt gatgtagacg agcgaccaga tccccttgga cggaaactcc atcaggccga
    14821 ccttgttgaa ggacgtgcca gggccggcag cgctcacgac cgacacgaag atctgcttca
    14881 acgcctgata gagattgcgc acgatcggca tgcgctggag gaacagctcg ccagagctga
    14941 tgatcgtgcg gccgagcagg ttggctgcca gcgcgccgat caacgtcagg ccgacgatgc
    15001 cgaacaagag cccgatgccg ggcatcggaa acggcaggta ggtctcggga ttatagatgc
    15061 ggggaacgaa cggcttcacc cacgcatcca ctacgttgat gaaccaccac gcgatgtaga
    15121 gcgtgatggt caccggcccg acgaccacca gccccgtcag gaagtagttg cgcaatcgcg
    15181 cgccaaggtg cagctccgaa tgggtctgca cgacgccggg gcgcggcggc tttgcctgcg
    15241 cactcggatt tgcgccaggc agcggctgct gctccgttcc gttgttctgc attattcgac
    15301 ttgtaaccct gccagcacca tcgcacgcgg cgcagggcct cccgatactt gcatcccccg
    15361 acagggaagc cggattagag ccgtcgcgca ataggcgtta agtccggtag gataccagac
    15421 cgcgagccgg aagcgccgat gggctctact gatcgacgat catgca
//
LOCUS       DASVDU010000203         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1718688, whole genome shotgun sequence.
ACCESSION   DASVDU010000203 DASVDU010000000
VERSION     DASVDU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718688"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF226_13050"
     CDS             complement(<1..510)
                     /locus_tag="VF226_13050"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX7074961.1"
                     /translation="MSLSQSVSGAFVKRLLSSIAIVPPFAAAAFSSAAAQTALELPGI
                     VVEGATLEAPPAAPQSKPVTKKASSSAPSAQTAAAPAGPAEAEGEVAANAGAEAEVDQ
                     VSGISTAKLGTAVTVVTGEDLRRQQIRHAADALRSLPGVTVNRTGSPAGLTQVRIRGA
                     DANHTLVLID"
     regulatory      complement(727..950)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1000..1476)
                     /locus_tag="VF226_13055"
     CDS             complement(1000..1476)
                     /locus_tag="VF226_13055"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX7074962.1"
                     /translation="MSAFVRLFGVLALSLGMLLPAPTMAQELRTYSKRANYDDVRFDL
                     TNAIISRGLNIEHTGNIAAMLERTGADVGSDKRLYKSAEFFTFCSAKLSRQMMEADVR
                     NIAFCPYVISIYESLGEPGVVVVSYRRPPASGTPETKAALAEIEALLDGIAREATQ"
     gene            complement(1498..1638)
                     /locus_tag="VF226_13060"
     CDS             complement(1498..1638)
                     /locus_tag="VF226_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074963.1"
                     /translation="MDQGLKKAVVGGAVAVLMLGAGYLYAVRGQAILMDLAAMGSALL
                     CL"
     gene            complement(1681..2910)
                     /locus_tag="VF226_13065"
     CDS             complement(1681..2910)
                     /locus_tag="VF226_13065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074964.1"
                     /translation="MTTRSSTARLPTWAIILTAGLIVGMAMGLRQVMGLYLKPMTTEL
                     DIGREPFSNAMAIANLVWGVGAVLAGAIADRYGAGRVIVGSALSTCAGLYLMYAAQSP
                     VELYVSGVLLGIGVCGTGVTALVGAVGRLVVPDKRLAAIASLGMAGGIGGFVAFPYAH
                     VLIEWLGWQMSLLVLAATAALMIPLAWPLSGKPATQHAAATKPQSLKEAFREAFAYPS
                     FWLLTAGFFVCGFHVAFYSVHLPAFVADKGLDASVGVWALMAVGIANIIGTYLAGQSG
                     RLVEKRVGLSVIYFMRCFVFLGLLFLPINGPMVILLSAMLGLFWLSTVPLTSGLVATF
                     FGTTWMSMLFGFVFLSHQLGSFGGLWLAGRLYDLTGSYNAMWWISVALGLFAAAVHWP
                     IRERPVARLAAERAVAA"
     gene            complement(3004..3990)
                     /locus_tag="VF226_13070"
     CDS             complement(3004..3990)
                     /locus_tag="VF226_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7074965.1"
                     /translation="MNAQITIAHLSDVHLAPLAAFWPVHWNAKRFLGFANWMVRRRKS
                     HLRAVVDLLVADLRHQGVDHICVSGDLINLGLPQEYEMALLWLHTLGPPDCVSVVPGN
                     HDIYTRLRRHTGVARWARYMESDEWGAALAPGAGPRFPYVRRINESIALVGLNSAVPT
                     PPGIASGEVGPKQIRECHRILRELGQMGMFRLVMIHHPPLVSQVLPGRDLKDAPELER
                     AIIDAGAELVIHGHNHRDMVGWHRTKTGLMPVVGIASASIGRVHRHEPLARYNIYRLH
                     TPRPGHDWQIELIGRGIREPDGPVVQVERRWLEPGHHAAPARTSHEAAASAR"
     gene            4107..4568
                     /locus_tag="VF226_13075"
     CDS             4107..4568
                     /locus_tag="VF226_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7074966.1"
                     /translation="MALELAVRKLLQRYWRMTRSLTVGAQGVVFDDAGRILLIRHTYR
                     PGWHFPGGGVEKNETVRAALHRELREEAGIIITGEPELIGVYANFRIFPSDHVMLFAV
                     RHWDQPSIPKANHEIAEQGFFHPDRLPEGTVGPVRRRIAELLHGAERTEWW"
BASE COUNT          880 a         1537 c         1480 g          734 t
ORIGIN      
        1 gtctatgaga acgagggtgt ggttggcatc cgccccgcgt atccgaacct gcgtcaatcc
       61 agccggcgag cccgtccgat tgaccgtcac ccccggcaag ctccgcaggg catcggccgc
      121 gtgccggatc tgctggcgcc gtaggtcctc gccggtgacc accgtcaccg cggtgccaag
      181 cttggccgtc gaaatacctg agacctggtc cacctcagct tcagctccag cgttcgcggc
      241 gacctcaccc tcggcctcgg ccggcccagc cggagcggcc gctgtttggg cagacggcgc
      301 agaactcgat gccttcttgg tgaccggctt gctttgcggc gccgccggcg gcgcttcgag
      361 cgtggccccc tcgacgacga tccccggcag ctcgagtgcc gtctgtgcag ccgccgacga
      421 gaacgcagcg gctgcgaagg gtggaacgat ggcaatggat gacaggagac gctttacgaa
      481 cgcaccggat accgattgcg acaacgacat gatttctccc cacggcgaat ggcgtgggag
      541 caccacagag caacaggtcc ggtccggttc cgagaaaaat gccgacgccg aagccgcctc
      601 agtcctcact gagacgcagc gccagtcaga gccgatacgc ctggcaaccc atgtctgacg
      661 tgtccccgcc ggttacacat cagtcgccgc tacggctcat ccgctccgac gcgcgccccg
      721 cacgtcggat gggcgacctg tgccggcagg tctcctggct cgcggctcgg acgcctcgtc
      781 cagccttccc ggccccctca tgtctgggcc agtggcatgt gtgaacgagg ctcgccgcct
      841 acagttgcgg gggcagccac ggattggggg ctcttgcagc ccgttaccgt gttccctttt
      901 cacccgcccg cgtcgttgcg cgaacgggaa ccggcacgct ctcaagcaat cagacgccca
      961 cgtgatcgtc aagtggcaga atgtgggccg tcgcaggcgc tactgcgtcg cctcgcgagc
     1021 gatgccgtcg agcaacgcct cgatctcagc cagcgccgcc ttggtttcgg gagtgccact
     1081 tgcaggcggc cgacgatagc tgacgacgac gacgcccggc tcaccgaggc tctcgtagat
     1141 ggaaatcaca taggggcaga aggcgatgtt cctcacatcg gcttccatca tctgacggga
     1201 gagcttggcc gagcagaagg tgaagaactc ggccgatttg tagaggcgct tgtcgctgcc
     1261 gacatcggcg ccggttcgct ccagcatggc ggcgatgttg ccggtgtgct cgatgttcag
     1321 gccgcggctg atgatggcgt tggtcaggtc gaagcggacg tcgtcgtagt ttgcccgctt
     1381 gctgtaggtc cggagctcct gggccatcgt cggagccggc agaagcatcc ctagcgagag
     1441 cgcgagcacg ccgaacaggc ggacgaatgc agacatctca tttccatgca gcgggattca
     1501 aaggcaaagc aaagcactac ccatcgcagc gaggtccatc agaatggctt gcccgcgcac
     1561 tgcgtagaga taaccggcac cgagcatcag cacggcgacc gcgccaccaa caacagcctt
     1621 tttgagccct tgatccatgg tttgcactat agcaccaacg cgctaatgaa atcgagccga
     1681 tcaggccgcc accgcccgct ccgcggccaa gcgtgcgacc ggccgctcac ggatgggcca
     1741 atgaacggct gccgcgaaca gccccagcgc caccgatatc caccacatcg cattatacga
     1801 ccccgtgagg tcgtagaggc ggcccgccag ccaaaggccc ccgaatgagc ccagctggtg
     1861 cgacaggaac acgaacccga acagcatcga catccaggtg gttccaaaga acgtcgccac
     1921 cagaccgctg gtcagcggca ccgtcgaaag ccagaacagg ccaagcatgg cgctgagcag
     1981 gatgaccatc ggaccattga tcggcaggaa cagcagcccg aggaaaacga aacagcgcat
     2041 gaagtagatc acgctcaggc cgacacgctt ctcgacgagc cggcccgact ggcccgccag
     2101 atacgtgccg atgatgttgg cgatgccgac ggccatcagc gcccagacgc cgacgcttgc
     2161 gtccagcccc ttgtcagcga cgaacgccgg cagatgcacc gagtagaacg ccacgtggaa
     2221 gccgcagacg aagaacccgg ccgtgagcag ccagaagctc ggataggcga acgcctcacg
     2281 gaacgcctcc ttgagcgact gcggcttggt cgccgccgcg tgctgggtcg cgggcttgcc
     2341 gctcaacggc caagccaacg ggatcatcaa tgccgcggtg gcggccagca cgagaaggct
     2401 catctgccag ccgagccact cgatcagaac gtgcgcataa gggaaggcca cgaagccgcc
     2461 gatgcccccg gccatgccga gcgaagcgat ggcggcaagc cgcttgtcgg gcacgacgag
     2521 gcggcccacc gccccgacga gggcggtgac gccggtgccg cacacaccga tgccgagcag
     2581 gacaccgctg acgtaaagct ccaccggcga ctgggcggcg tacatcaggt agagcccggc
     2641 gcacgtcgac agcgcgcttc cgacgatgac gcggccggcg ccgtacctgt ccgcgatggc
     2701 gcccgccagc accgcgccga cgccccagac gaggttggcg atcgccatgg cattggagaa
     2761 cggctcgcga ccgatgtcga gctccgtcgt catcggtttg aggtagagcc ccatcacctg
     2821 ccgcaggccc atcgccatgc cgacaatgag accggccgtg agaatgatcg cccatgtcgg
     2881 caagcgggca gtcgaagatc gagtggtcat gagcacttcg cctggattcc ggatcggcca
     2941 cgttatggca caatcgcggc gtgaggtaag tcgaggattt cacatgagct tgatgagcaa
     3001 atcttatctg gctgacgccg ccgcttcgtg cgacgtccga gccggcgctg catgatggcc
     3061 cggctcaagc cagcggcgct caacctgcac gacaggcccg tccggctccc ggatgccgcg
     3121 gccgatcagc tcgatctgcc agtcgtgacc gggtcttggt gtatgcaatc ggtaaatatt
     3181 gtagcgcgcg agcggctcat gccggtgcac gcggccgatg gaggcggacg cgatgcccac
     3241 cacgggcatg aggccggtct tcgtccgatg ccagcccacc atgtcgcgat gattgtggcc
     3301 atgaatgacg agctccgcac ccgcatcgat gatggcgcgc tcaagttccg gcgcatcctt
     3361 gagatcgcgc ccggggagca cctgcgagac gagcggcgga tgatggatca tgaccaggcg
     3421 gaacatcccc atttgcccga gctcgcggag gatgcgatgg cactcgcgga tctgcttggg
     3481 gccgacctct ccggaggcga ttccgggcgg cgtcggaacg gccgaattga gcccgacgag
     3541 cgcgatcgac tcgttgatcc tgcggacgta aggaaagcgc gggcccgcac cgggagccag
     3601 cgcggcgccc cactcgtccg attccatgta gcgggcccaa cgtgcgaccc cggtatgccg
     3661 gcgcaggcgc gtgtagatgt cgtgattgcc agggacgacg gagacgcaat ccggtggccc
     3721 gagcgtgtgc agccacagca gcgccatctc gtattcctgc ggcagcccga ggttgatgag
     3781 atcgccggaa acgcagatat ggtcgacgcc ctggtggcgc agatcggcca cgagcaggtc
     3841 gacaacggcg cgcagatgtg actttctgcg ccgcaccatc cagtttgcga accctaggaa
     3901 cctcttggca ttccagtgaa ccggccagaa cgcagccagc ggcgccagat ggacgtcaga
     3961 gagatgtgcg attgtgatct gagcgttcat cgaatcgggt cccgggagag ctgctgcccg
     4021 cccggactgt taacacagtt cggacatcgg gggctccccc tattcgcctg ccgccgcaat
     4081 tggccgcttc tcgtggagca cgcaccttgg ccctggagct agcggttcgc aagctgttgc
     4141 agcgctattg gcgcatgacg cgctcgctca ccgtgggcgc gcagggcgtc gtcttcgatg
     4201 acgcaggccg aatcctcctg atccggcata cttaccggcc cggctggcat ttccccggcg
     4261 gcggcgtcga gaagaacgag acggtccgcg ccgcgctaca ccgtgagctg cgggaggaag
     4321 ccgggatcat catcaccggg gagcccgagt tgatcggcgt ctatgccaac ttccggatct
     4381 ttccgtcgga ccacgtcatg ctctttgcag tccgccactg ggatcagccc agcattccca
     4441 aagctaacca cgagatcgcc gagcagggct tctttcatcc ggatcgcctg ccggagggca
     4501 ccgtcggccc ggtccgcagg cgtattgcgg aattgctcca cggcgccgag cgcacggaat
     4561 ggtggtgagg agcagcgctc ttcgtgatgc tggaccaacc cgcgggctcc tgctacagca
     4621 tgcgcccatg a
//
LOCUS       DASVDU010000204        14009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_981666, whole genome shotgun sequence.
ACCESSION   DASVDU010000204 DASVDU010000000
VERSION     DASVDU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14009
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981666"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(329..1924)
                     /locus_tag="VF226_13080"
     CDS             complement(329..1924)
                     /locus_tag="VF226_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HEX7074967.1"
                     /translation="MLFTFGSYSIDCDRRELMRAGRLVAIEPQVFDVLVYLIRHRDRV
                     VSRDDLLSEVWGGRIVSESTLSSRINSARVAIGDSGDKQDWIRTVARKGFRFVGLVHE
                     ERGPIGRDERLIARPGLLETTASSEAEASPPTFALPDRPSVAVLPFDADPEIDGFADG
                     LTEDIITGLSRIKALWVIARNTMFTYKGRAIDVRTVGKELGVQYVVNGRVRKAGGRLR
                     MTAQLVETQTGHHVWAAKIDRDSTGLFELQDDLTQFVVASVQVQLIVSEGKSVARKAK
                     TVSRVDQLLTRALGRLYQPMADGLREMVSLAERALALDPTNGEACRLLAAGIWHQAYR
                     GIIPWDRSAADRVMMFAQRAVAAEETDEYSHWMLALAHLMMCQHDRAIASLRRALDIN
                     PSFSLGYGTLGTVLAWGGEPDESIANNELALRINPSDPLNAHRHFGLALAHYLAARYT
                     KALEHAVVVVEIRPDWWLALIMYAASLAQVGRVADAQLACIDLLRAKPDMTLASLNAL
                     PFAKRADRDHVADGLRKAGLPDG"
     gene            2229..2879
                     /locus_tag="VF226_13085"
     CDS             2229..2879
                     /locus_tag="VF226_13085"
                     /inference="COORDINATES: protein motif:HMM:NF042551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6639 family protein"
                     /protein_id="HEX7074968.1"
                     /translation="MSPPTKVAKPTNMEPCPDAPVDVAAESPDERRLACSAAAEAIQR
                     LAACGITPRRPVRFQIMSEVRHPLSGTIFGLFDPKQERVLVTHETNVPALVKGTPYAA
                     LPQRDFYRSLIIHEVVHAVMHQNLKRPASSHAAYEYPAYALQIDSLTPGVRDMFLRSF
                     GQRAIKANSIFSDSTLFFDPYFFAARAYLHFKASADGCSHLTAILEDEISFIAPPM"
     gene            3147..3551
                     /locus_tag="VF226_13090"
     CDS             3147..3551
                     /locus_tag="VF226_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7074969.1"
                     /translation="MSTRTASCSCGQLRIEVYGEPLGVGVCHCLACQRRTGSVFAALA
                     SFAAPYTVHGTATEYVRTGDQGARFRFRFCPVCGTTVFHTEEGYEDRSVAVAVGAFAD
                     PDFPPPKVSVYDCRRHSWVQLPSGITTYDKDP"
     gene            complement(3686..4594)
                     /locus_tag="VF226_13095"
     CDS             complement(3686..4594)
                     /locus_tag="VF226_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7074970.1"
                     /translation="MNIRDLEAFIAVVETGSIISASARLNLTQPGVTRRIQNLEERLG
                     AVLLDRQSKPLKPTPAGREAYEHGRRVLRTVEDLKVSVSPSGEIQGEFRVGVMPYFSE
                     AALTIPLDRLRACFPRLTLRVTSAWSIRLLEQLNRNELDAAVLCLGHGDSPPENLLGH
                     DLGTQEVLIVAAPGLGAPSPASLADLSRFPWVMNESGCGFRTYIRRQFEKARLPFQVG
                     VEALSADLRMSLVARGHGIGIVTPAAFASSPWRDQVRVIDTIDFHPRVQSWVLHREPT
                     GWLNRPIAVLLEAIIEALHSPAPFTA"
     gene            4693..5919
                     /locus_tag="VF226_13100"
     CDS             4693..5919
                     /locus_tag="VF226_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7074971.1"
                     /translation="MSPRDTAFAESTTPTATSSASKPPTLLFATAAGVIALNLFAPQT
                     LVGVVAPSIDLDPRLGGLVAMAPLVGYAAGLLLLVPMADLVENRALVLRMLILAILAS
                     VMATIAPNAAMLLPVLTVLGAMCAAIQILVPMAAAMAAPESRGRVIGDVMGGVLAGIV
                     LARPVASVITEAWSWRAFYATSAVAMALIALIVTPRLPRLHPQVRTGYVALLASLWHI
                     LRSEVVLRRRAMTAGLVMAAFSLFWTCIALRLSLAPFELGQRGIALFALVSAGGALAS
                     PAFGRVGDRGWTRSATTLSHAMLLGSLALAAWAETAALDTRLALVLMALGALLLDIAI
                     TGDQTLGRRAVNLLRPEARGRINGLFVGLFFIGGAIGSAIAGFVWAYGGWNAACAVAA
                     LFGIAALIIGRLEKGV"
     gene            complement(6133..6567)
                     /gene="msrB"
                     /locus_tag="VF226_13105"
     CDS             complement(6133..6567)
                     /gene="msrB"
                     /locus_tag="VF226_13105"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7074972.1"
                     /translation="MVDKLRLSEEEWRRLLSPEAYRVLRQHGTEPPFEGCFLGTHEPG
                     TYVCAACGNPLFRSGEKFESGTGWPSFTRPVSEDAVIEHEDRSYGMERTEVRCARCDS
                     HLGHVFPDGPPPTGLRYCMNSVAMKHVPQGEPIPLVMAPSDQ"
     gene            complement(6610..7227)
                     /gene="msrA"
                     /locus_tag="VF226_13110"
     CDS             complement(6610..7227)
                     /gene="msrA"
                     /locus_tag="VF226_13110"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7074973.1"
                     /translation="MGLTIETRSFPDPAEDLNADASSGTLVLAGGCFWCTEAVFELLE
                     GVDSVRPVYAGGSAKTANYQAVCTGQTGHAEAIEIRYDPSEISYGQLLKIFFSVAHDP
                     TQLNRQGADVGTQYRSAIFYATEDERRVAEAYIHQLDEAGVFDTPIATILEPLDQYFP
                     AEAIHCRYAQRNPDQPYIRAVSDPKVRKLKEVFAGALKPEVRSGR"
     gene            8007..8543
                     /locus_tag="VF226_13115"
     CDS             8007..8543
                     /locus_tag="VF226_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7074974.1"
                     /translation="MKLNRGGVIGPAIMAACIGIATTPAIAADFGGPRYAEPPMEMPF
                     SSIWQGLYGGVHLGYGDAGHTDGVVGGGQLGYNWQANRFVYGLEGDISVTDTSVDWLA
                     SVRGRAGILLDDRLLAYGTAGVGFADIHDDTETDFVYGLGLESKLTEAMTARLEYLNY
                     NDIDVDVIRAGLNFKFGY"
     gene            complement(8617..9909)
                     /locus_tag="VF226_13120"
     CDS             complement(8617..9909)
                     /locus_tag="VF226_13120"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7074975.1"
                     /translation="MANVVVIGAQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLV
                     INGETYKLSLLPSGVVRPGKLAVIGNGVVIDPWALAEEIERLQKQGVAVSRDNLRIAE
                     NATLILPLHRELDMMREQAAGDGRIGTTGRGIGPAYEDKVGRRAIRVQDLKNLGTLGP
                     KVDRILVHHNALRRGLGQPEIAKDELMRELTEIAPKILPYMDVTWALLDARRKAGDRI
                     LFEGAQGALLDVDHGTYPFVTSSNTVAAQAATGSGIGPAAINYVLGIAKAYTTRVGAG
                     PFPTELHDATGDEIGQRGREFGTVTGRKRRCGWFDACLVRQVTKVSGIHGIALTKLDV
                     LDGFSEVKVCVGYMLDGERIDRLPAGHNAQARVQPIYETFEGWAQSTRGARSWADLPA
                     QAVKYVRTIEELIECPVTLLSTSPEREDTILMKDPFAD"
     gene            complement(10002..11678)
                     /locus_tag="VF226_13125"
     CDS             complement(10002..11678)
                     /locus_tag="VF226_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEX7074976.1"
                     /translation="MPVLLGVVMLLAAVLPLRAAELPVTSRVEAVTVYPSGAEVRRVV
                     RLQLEAGDHTVVIGDLPQQAVESSIRVEGKATGKLEIGAVDSRRIVVERANAEAQRTE
                     RRRIENEIERLKDERASFEARIETAETQKAFVTRLAELPTRPIAPQPAGQIPPHEDWS
                     EILTLIGSSLADVHRAIQDSQIRIREIDRRIEELEKTLADQAPAVEERTEVKVHLTAA
                     APLEADLVVRYQVPNASWTPLYDARLMTGSKNVPPKLTITRRATITQRTGEAWENVAL
                     SLSTARPASNVSAPELRPITVDFEPEPRPMAEAAPEMESMQRRRSASRTLGAAPPPAP
                     AADMAAAKEQYAAASISEFAATFSVPGRVTVANTGEVKRVAIDETQLDPTLLVRAVPK
                     REERAFLYAKLKLPRGSVFLPGQISLFRDQTFVGTGRLPQLSGGDEHELGFGTDDAVR
                     IRYAVADEKRGETGLISTSRTDQRNYRITVKNLHERPIAFTVLDQIPTSLNQDIKVEL
                     LGGTAPTRREVDGKRGVLAWEGELAPDEERAIEFGYRITWPAAKNITFRP"
     gene            complement(11779..13359)
                     /gene="serA"
                     /locus_tag="VF226_13130"
     CDS             complement(11779..13359)
                     /gene="serA"
                     /locus_tag="VF226_13130"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX7074977.1"
                     /translation="MTPKVLISDELSPAAIAVFKKRGVDVDVKVGLSKDQLEKIIGDY
                     DGLAVRSATKVTEKIIAAGKRLKVIGRAGIGVDNIDTRAATARGIIVMNTPYGNSITT
                     AEHAISLMLALARQIPQADRSTQAGKWEKSKFMGVELFGKTLGIIGCGNIGSIVADRG
                     IGLKMRVIAYDPFLSPERAIEIGVEKVELDELFQRADFITLHTPLTERTRHIIDAKAL
                     SKMKPTVRIINCARGGLIDEAALAEALKAGRVAGAALDVFEVEPARESPVFGLDNVIC
                     TPHLGASTTEAQENVALQVAEQMSDYLLKGAISNAINFPNITAEEAPKLTPFIKLAEQ
                     LGMFAGQLTETRLKQVRIEYIGEIAEMNTRALTAAMLAGLLRPQLTEVNMVSAPAMAR
                     ERGLAVEEVTRGEEGAYLTYARLTVQTERGERSVAGTVFSDGRPRIIQIKGIGMEAEL
                     SPHMLYITNQDKPGFIGKFGSLMGEAGLNIATLNLGRDRPGGDAICLVALDEPVSDEI
                     LAKVRALPMVTRAHRLEF"
     gene            complement(13680..>14009)
                     /locus_tag="VF226_13135"
     CDS             complement(13680..>14009)
                     /locus_tag="VF226_13135"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450717.1"
                     /note="catalyzes the formation of 3-phosphonooxypyruvate
                     and glutamate from O-phospho-L-serine and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine aminotransferase"
                     /protein_id="HEX7074978.1"
                     /translation="AIFDWVERTPWIANLARDPATRSNTSVCLSIVDPAVTRLSVDQQ
                     WAFAKQIAALLDKEGVAHDIAAYRDAPPGLRIWTGSTVERSDVEALLPWLDWAFATAR
                     ASLSKAA"
BASE COUNT         2478 a         4601 c         4321 g         2609 t
ORIGIN      
        1 ttccattccc acgtcgagcg cgacgccttc gtccatgact tcgtccaccg ctacaatcac
       61 acacgtctca agtgcctcga ctatcgggcc cccgcagagc tcatctccaa tcctccggga
      121 ctgaacacgc aggcggggat ccacgacacg ctgccacaag cgctcgttat ctgaggtacg
      181 ctaccgacgt cccaacaccc tccgcgagcc accgatgccc acccgaaccg tctcctgctc
      241 ctgcggtcag acttgagtac tacttttaac ccaaagccct tttgatgagc gcgctacacg
      301 cccggaacaa gtaagccgaa attgtctttc atccgtcagg cagcccggct ttacgcagac
      361 catcggcgac gtggtcgcgg tcggcacgct ttgcgaaggg aagagcattg agagaggcca
      421 gagtcatgtc tggctttgcg cgaagcaaat cgatgcacgc cagctgcgca tcggcgacac
      481 gaccgacctg cgccaggctc gccgcataca tgataagtgc gagccaccag tccgggcgaa
      541 tttcgaccac gacgacggcg tgctccaagg ccttggtgta gcgggcagcg agatagtgcg
      601 ccagcgccag accgaagtgg cggtgggcat tcaatggatc gctcggattg atcctcaacg
      661 cgagctcgtt gtttgcaatc gactcatcgg gttcaccacc ccaggccagc acggttccta
      721 acgtcccgta ccccagcgag aagctcggat tgatatccag cgcgcgcctc agcgacgcga
      781 tcgcccggtc gtgctggcac atcatcaggt gcgccagcgc cagcatccaa tgcgagtatt
      841 cgtccgtctc ctcggcagcg accgcgcgct gagcgaacat catcaccctg tctgccgccg
      901 atcgatccca cggtatgatg cccctgtagg cctgatgcca gatgccagcg gcaagcaggc
      961 ggcaggcttc gccgttggtc gggtcaagtg cgagcgctct ttcggcgagc gataccatct
     1021 ccctcagccc gtcagccatc ggctgataga gccgccccaa tgcgcgtgtc aggagttggt
     1081 cgacgcgaga gactgtcttt gcctttcgcg cgaccgattt gccttcactc acgatgagct
     1141 gcacctgcac cgaggccacc acgaattgcg tcaagtcgtc ctgcagctcg aacagccccg
     1201 tgctatcgcg gtcgatcttg gcggcccaga cgtggtgacc agtctgcgtc tcgaccagtt
     1261 gcgccgtcat gcgaagccga cctccggcct ttcgcaccct cccattgacg acatactgca
     1321 cacctagctc cttgcccact gtgcgcacgt cgatcgcccg ccctttgtag gtaaacatcg
     1381 tattgcgtgc gatgacccaa agggccttta tgcgcgacaa gcccgtaatg atgtcctcgg
     1441 tcaatccgtc ggcgaacccg tcaatctccg ggtcagcgtc gaagggaagt acggcgacgg
     1501 aaggccggtc ggggagtgca aaggtcggcg gagaagcttc cgcttcgctg cttgctgtgg
     1561 tctcgagaag accgggcctg gctatgagcc gttcgtcccg tccgatcgga ccgcgctcct
     1621 catgaaccag gcccacgaag cgaaaaccct tgcgggcgac ggtgcggatc caatcctgct
     1681 tatcgccgct gtctccgatc gccacacggg ctgaattgat gcggctggac agggtcgatt
     1741 cggaaacaat gcggccaccc cagacctctg acaaaagatc atccctgctg acgacgcgat
     1801 cccgatggcg gatcagatag accagcacgt cgaagacttg cggctcgatc gccaccagcc
     1861 gaccagcgcg catgagctcg cggcggtcgc aatcgatcga gtagctgcca aacgtaaaca
     1921 gcatcgactt aatcttgaac tgaggcttct agaaaccctc tagccggaca atcaaagcat
     1981 tctagaacaa aaatcgcatc gctctcgaag cgcagggaac agcgcgtcgg catcctacac
     2041 cctacggtcg aaccgttggc cgtttaccag atcgggaggt aacgatgcga cgcactgcct
     2101 gttggagcat cctttcgctc cttggttacc ttgtggtcca acaagcgcaa gcgcagccgg
     2161 ctcaatgccg ggctgacgac ctctgcatcc cggcagaggc gataaccgcc cccgaaaagg
     2221 tggatcgggt gtcgccgccc acgaaggtgg ccaaaccaac caatatggaa ccgtgcccgg
     2281 acgcgcctgt cgatgtcgcg gcagagtctc cagacgagcg tcggctcgcg tgttctgcgg
     2341 ccgccgaagc tatccagcga ctcgccgcct gcggcataac gccgcgccgg cctgtccggt
     2401 tccagatcat gagcgaggtc cggcacccct tgagcggcac gatcttcggc ctcttcgacc
     2461 cgaagcagga gcgggtgctc gtcacccacg aaaccaatgt cccggccctc gtaaagggta
     2521 ctccgtatgc cgcgctgccg cagcgcgact tctacagaag cctcatcatc cacgaggtcg
     2581 tccatgcagt catgcatcag aacttgaagc ggccggcatc gagccacgcg gcctacgagt
     2641 atcctgccta tgcgctccag atcgattctc ttaccccagg tgtgcgcgac atgttcttac
     2701 ggtcgttcgg tcaacgagcg atcaaagcca attcgatatt cagcgattcc accttgttct
     2761 tcgacccgta cttctttgcc gcgcgggcct atctgcactt caaagcctct gctgacgggt
     2821 gctcccatct tactgctatc ttggaggacg agatttcctt tattgctcca ccaatgtagg
     2881 tgcctcgacg ctgtaacgtg aaccatcgat aattccggat ccgaactttc cttgctgagg
     2941 gttcggtcta cgcctccaat ccacgtccca cgcgtgggta cttgcgtaga tccccggcct
     3001 acgccgggat gacggaagag tggatgctgc gccagtgcca ccccccacac cgtcatcccc
     3061 gcggaggcgg tgatccacga cacgctgcca caatcgctcg ttatctgagg aacgctacca
     3121 acgtcccaac acccaccaag ccacacatgt ccacccgaac cgcctcctgc tcctgcggcc
     3181 agttgcgcat cgaggtctac ggcgagccgc taggcgtcgg cgtatgtcat tgcctcgcct
     3241 gtcaacggcg gacgggcagc gtgttcgcgg cgctcgccag cttcgccgct ccgtatacgg
     3301 tccatggcac cgcaaccgag tatgtgcgca ccggcgatca gggcgcccga ttcaggttcc
     3361 gcttctgccc cgtctgcgga acgaccgtgt tccacaccga ggaaggctac gaggacaggt
     3421 ccgtggccgt cgccgtgggc gcgtttgccg acccggactt tccgccgccg aaagtttcgg
     3481 tctacgactg ccgcaggcac tcctgggtgc agctgccgtc ggggattacg acctacgaca
     3541 aggacccctg actgacagcc ggcggcaacg gcagtcactc ccctcacctc ttctgagggc
     3601 agagaacccg cccagctttc atccgacagc cgcctgaaag ccggtgagac atcgcaacaa
     3661 ttgctaccgg tggaacccgc cggggtcatg cggtgaacgg cgccggactg tgaagcgctt
     3721 cgatgatcgc ctccaggagg accgcgattg gacggttaag ccatcccgtg ggctcacggt
     3781 gcaaaaccca agattggacc cgcgggtgga aatcaatcgt atcgatcacc cgaacctgat
     3841 cgcgccaagg actcgaagca aatgcagctg gcgtgacgat tccgataccg tgtccgcgcg
     3901 ccacaagcga catgcgcagg tcggcgctca gcgcctcgac accaacctgg aagggcagcc
     3961 gcgccttctc gaattggcgg cgaatgtagg tgcgaaaacc gcatccgctc tcgttcatca
     4021 cccaaggaaa gcgcgaaagg tcggcgaggc tcgccgggct cggtgcaccg aggccggggg
     4081 cagcaacgat gagcacctcc tgcgttccga ggtcatgtcc gagcaggttt tccggcgggg
     4141 aatcgccatg gcccaggcaa aggaccgcgg cgtcgagctc gttgcggttg agttgctcca
     4201 gcagccggat cgaccaggcg gacgtgacgc ggagcgtcag tcgaggaaag catgcacgca
     4261 aacggtcgag gggaatcgtg agagcggctt ccgaaaaata cggcatcacg ccgacgcgga
     4321 actcgccctg gatctcgccg gacggcgaaa cgctgacttt gagatcttcg accgtgcgca
     4381 agacgcgccg accgtgctcg taagcctccc gtcccgccgg cgtcggcttc aagggcttcg
     4441 attggcggtc gaggagaacg gcgccgagcc gttcctccag gttctggatg cggcgcgtca
     4501 cgccgggctg ggtgaggttc agccgagccg aggcggagat gatcgacccg gtttcgacga
     4561 cggcaatgaa ggcttcgagg tcacggatgt tcatgcgtga ttcgcataat ctttatagaa
     4621 tgaattaaat tgtcgcataa tgagcgcgtg acgtaaacag cccgacgaca ttgactgtcg
     4681 aagggaaacc agatgtcccc acgcgatacc gctttcgctg aatccacgac gcccacggcc
     4741 acttcctccg caagcaaacc gccgacactc ctctttgcga cggcggctgg cgtcatcgcg
     4801 ctcaacctct tcgcgccaca aacgctggtg ggggtggtcg cgccttccat cgaccttgat
     4861 ccacgccttg gcgggctcgt ggccatggcg ccactcgttg gctatgcggc cgggctgctg
     4921 ctcctcgttc cgatggcgga tcttgttgag aaccgggcgc tcgtgctgcg gatgctgatc
     4981 ctcgccatcc tggcctcggt gatggcgacc atcgcaccca atgcggcgat gcttctcccc
     5041 gtcctcacgg ttctcggcgc catgtgcgcg gcgatacaaa tcctcgtgcc catggccgca
     5101 gcaatggcgg cgcccgagag ccgcggccgc gtcattggcg atgtcatggg cggcgttttg
     5161 gcgggcatcg tcctggcacg accggtcgcc agcgtcatta cggaggcatg gagctggcgg
     5221 gccttctatg ccacgagcgc cgtggctatg gcgctcattg cgctgatcgt gaccccgcgc
     5281 ctaccccggc tgcatcccca agtccggact ggatatgtcg cgctgctcgc ctcgctgtgg
     5341 cacatcctcc gcagcgaagt tgtcctgagg cgaagggcga tgaccgctgg cctcgtgatg
     5401 gcagcgttca gcctgttctg gacgtgcatc gcgctgcgcc tctcgctcgc ccccttcgag
     5461 cttgggcagc gaggcatcgc cctgttcgcc cttgtaagcg caggtggtgc attggccagc
     5521 cctgcgttcg ggcgagtcgg cgaccggggt tggacacggt ccgccacaac cctgtcgcat
     5581 gccatgctgc ttggttccct ggcgctcgcg gcgtgggcag aaaccgcggc tcttgacaca
     5641 cgtcttgccc tggtgctgat ggctctcggc gctctgctgc tcgacatcgc cattacgggt
     5701 gatcagacgc tcggccgccg tgcggtgaac ctcctgcgcc cggaggcgcg cggccgaatc
     5761 aacgggctgt tcgtcgggct gttctttatc ggcggcgcaa tcggctccgc gattgcaggc
     5821 ttcgtctggg cctacggcgg ctggaacgcc gcctgcgccg tagcagccct attcggaatc
     5881 gcagcgctca tcatcggccg tctggagaaa ggggtctgaa ccgaggcccc tcactcgtca
     5941 tgcgagctgg cgggctcgac cggaatgtcg aaatgcaaca tgtggatcgt cgccgtaatc
     6001 ggactggacg tggatggtgg acattggcgt tcctcgacgg gcgttgatct tccccggctt
     6061 cactcctcga caaatcttct gaggcgcgcg acggcctcat cccactgctc gcgcggctat
     6121 tgccgccctc ttttactgat ccgacggcgc catcaccagc ggaatgggtt caccttgagg
     6181 cacgtgcttc atggcgaccg aattcatgca atagcgcagg ccagtgggcg gcggtccgtc
     6241 agggaagacg tggcccaaat ggctgtcgca ccgggcacag cgcacctctg tgcgctccat
     6301 gccgtaggag cgatcctcgt gctcgatgac ggcatcctcc gaaaccggcc gggtgaacga
     6361 cggccatccg gtgccggact caaatttctc acccgatctg aaaagcggat tgccacatgc
     6421 ggcgcagacg taggtccccg gctcgtgcgt accgaggaag cagccttcga agggcggctc
     6481 cgtgccgtgc tgccggagca cgcgatacgc ctcgggcgag aggagccttc gccattcctc
     6541 ttcgctgagt cgtagcttgt cgaccatgat ctcctccttt tccggtctcg acagacgcac
     6601 ggtcggccat caccggccgc tgcggacctc cggtttgagc gcgccggcga acacctcctt
     6661 cagcttccga accttgggat cggaaaccgc ccggatatag ggctggtccg ggttccgctg
     6721 cgcgtatctg cagtggatcg cctccgccgg gaagtactga tcgaggggct cgaggatcgt
     6781 tgcgataggg gtgtcgaaaa cccccgcctc atcgagctgg tggatataag cctcggcgac
     6841 gcggcgctcg tcctctgtgg cgtagaagat ggccgagcga tactgagtgc ccacgtcggc
     6901 accctgccgg ttcagctgtg tcgggtcgtg cgccaccgaa aagaagatct tcagcagctg
     6961 gccatagctg atttcgctcg ggtcgtaacg gatctcgatc gcttccgcat gtccggtctg
     7021 gccggtgcac acggcctggt agttcgcggt cttcgccgat ccgccggcgt aaaccgggcg
     7081 aacggagtcc acgccctcca gaagctcgaa caccgcctcg gtgcaccaga agcagccgcc
     7141 tgccagcaca agcgtcccag atgaggcgtc cgcgttcaaa tcctctgcgg gatcggggaa
     7201 actgcgcgtc tcaatggtaa ggcccatgtc atctttcctt gcggatcgta gctggcgatc
     7261 cgtcttgtta cagccgctga cacatgccgc gaacgggatc gaatcgcgtc tggttccgac
     7321 ccgcaccgtc gcacgcacca gaacgaacgg catcagcgct ggcgatcgcg agatcgcgtg
     7381 gagaggctgg cagggaactt cgccgcgtac cgccccctat ttcagggggc tgtaaagggg
     7441 ccgtgacctg gttattcggt ttgaccacgc gccggtaaag gtgccgactc gcatgaccga
     7501 gcaccggcat tgcgatggcc aaaaccgacc ggaatggcag ctatccaatg accattcccg
     7561 ccgcgatggt gcgctcgagg cggcgcttgc cggcctcgat gtccaagcga ggacatacgc
     7621 gatgggcgcg caaggcccgt cgcccgacgg aagcgccgtc ttgaatgttt gcttggacat
     7681 cccagatcgg ggaatcatct tctcgaccaa gctggcgacg gaggaatgcg tcgcaaacca
     7741 ttcctgcgcg gcgtcgcggc aagcgcagcg acttctcgga accgcgtgat ccttcgacca
     7801 cataaatgct caaaagcgaa tgaaacaaaa cccatctaat cctttgaaat tgcccatgtt
     7861 tttaatcagt tcccggatcg ccgctccgag agcgtccgcc ggcttgctcc gtaccggcgc
     7921 cacaaacatt gcctaattcc tgacgggcag acaggagcgc gggtggcctg agggggaggc
     7981 tgcgttcccg atcaggagga ccacttatga agttgaatcg agggggagtt atcggaccgg
     8041 cgatcatggc cgcatgtatc ggtatagcca cgaccccggc aatcgcagca gacttcggcg
     8101 gcccccgcta cgccgagccg cctatggaaa tgccattcag ctcgatctgg caggggctct
     8161 acggaggcgt gcacctcggc tacggcgatg ccgggcatac ggacggcgtc gtcggtggcg
     8221 gccagcttgg ctacaactgg caggcaaacc ggttcgtcta tggtctcgag ggtgacatct
     8281 cggtcaccga cacgtccgtc gactggctcg cctcggtgcg gggccgcgcg ggcattctgc
     8341 tcgacgacag gctgctcgcc tacggcaccg caggtgtcgg cttcgctgac atccacgacg
     8401 acaccgagac ggacttcgtc tacggcctgg gtctggagag caagctcacc gaagcgatga
     8461 cggcccggct cgaatacctg aactacaacg acatcgacgt cgacgtcatc agggctggcc
     8521 tgaacttcaa gttcggctac tgacagcccc gcaagagacc gcagggcacg ggatccgatg
     8581 gagccatcga cctcgtgccc tgtgcttttg cggaagtcag tccgcgaacg gatccttcat
     8641 caggatggtg tcctcgcgct cggggctcgt cgacagcagc gtgacggggc actcgatcag
     8701 ctcctcgatc gtgcggacgt acttgacggc ttgcgccggc aggtcggccc aggagcgcgc
     8761 accgcgggtg gattgagccc agccctcgaa tgtttcgtaa atgggctgga cgcgcgcctg
     8821 ggcgttgtgg ccggccggta gccggtcgat gcgctcgccg tcgagcatgt agccgacgca
     8881 cactttcacc tccgagaagc cgtcgaggac gtccagcttc gtcagcgcga tgccgtggat
     8941 gcctgatacc ttcgtgacct gacggacgag acaggcatcg aaccagccgc agcgccgctt
     9001 gcgacccgta actgtgccga actccctgcc gcgctggccg atttcatcgc ccgtcgcgtc
     9061 gtgaagctct gtcgggaacg ggccggcccc aacgcgggtc gtgtacgcct tggcaattcc
     9121 gaggacgtag ttgatggcag ccggtcctat gccggagccg gtcgccgcct gggccgccac
     9181 cgtgttcgag gacgtcacga aagggtaggt cccgtggtcg acgtcgagca gcgcgccctg
     9241 cgcaccctcg aacaggatgc gatcgcccgc cttgcgcctc gcgtccagca aagcccaggt
     9301 gacgtccatg tacggcagga tcttcggggc gatctcggtc agctcccgca tcagctcatc
     9361 cttggcgatc tccggctggc caaggccgcg gcgaagcgcg ttgtgatgca cgaggatacg
     9421 gtcgactttc ggccccagcg tgccgaggtt cttaaggtcc tgcacgcgaa ttgcgcggcg
     9481 gccgaccttg tcctcgtagg ccggaccgat gccgcggccc gtggtgccga tgcggccatc
     9541 gccggcggcc tgctcgcgca tcatgtcgag ctcgcgatga agcggcagaa tgagagtcgc
     9601 attctcagcg atgcgcaggt tgtcccgcga gaccgcgaca ccttgcttct gcagccgctc
     9661 gatctcttca gccaaggccc acggatcgat gaccactcca ttgccgatga cggcgagctt
     9721 gcccggacgc accacacccg acggcagcag cgacagcttg taggtctcgc cgttgatgac
     9781 gagcgtatgg ccggcgttat ggccaccctg gaaacgcacc accacgtccg cgcgctccga
     9841 cagccagtcg acgatcttgc ccttgccctc gtcgccccac tgggcgccga tcaccaccac
     9901 gttcgccatc agtcgtccct tgtcgcgaaa cgaccccggc tcatacgccg gggtcttaaa
     9961 gagactctat agctcaacag ccacgcgggt ggaccaatca atcagggccg gaacgtgatg
    10021 tttttcgccg cgggccatgt gatgcgatag ccgaattcga tcgcgcgctc ctcgtcgggc
    10081 gcgagctccc cctcccaggc gagaacgccg cgcttgccgt cgacctcgcg ccgggtcggc
    10141 gcggtgccgc ccaggagctc gaccttgatg tcctggttga gcgaggtcgg aatctggtcg
    10201 agcaccgtga acgcaatggg ccgttcgtgg aggttcttga ccgtgatccg gtaattgcgc
    10261 tggtcggtgc gcgaggtgga gatcagcccc gtttcgccgc gcttttcatc ggcgaccgca
    10321 tagcggatgc gcacggcatc atccgttccg aagcctagct cgtgctcgtc accgcccgac
    10381 aactgcggga gtcgcccagt gccgacgaac gtctggtcgc ggaagagcga gatctgcccg
    10441 ggaaggaaca cgctcccccg gggcagcttc agcttggcat agaggaatgc gcgctcctcg
    10501 cgcttcggga cggcacgcac cagcagtgtc ggatcgagct gcgtctcgtc gatggcgact
    10561 cgcttcactt cgcccgtatt ggcgacggtg acgcgtcccg gcaccgagaa ggttgccgcg
    10621 aactccgaga tgctggcagc cgcatactgc tcctttgcgg ccgccatgtc ggcggcagga
    10681 gctggcggag gtgccgcgcc gagtgtgcgc gacgccgaac gccttctctg catgctttcc
    10741 atctcgggag ccgcctctgc catgggccgc ggctcgggct cgaaatcgac cgtgatcggc
    10801 ctgagctccg gagcagagac gttgctggcc ggccgtgccg tcgacaacga gagggctacg
    10861 ttctcccagg cctcgcccgt tcgctgggtg atggtggccc gccgcgtgat ggtgagcttc
    10921 ggtggcacgt ttttcgagcc ggtcatgaga cgggcatcat agagcggcgt ccaggaagcg
    10981 ttcggcacct gatagcgcac gacgaggtcg gcttcgagcg gcgccgccgc ggtcaagtga
    11041 accttgacct cggtacgctc ctcgacggcc ggggcctggt ctgccagcgt cttctcgagc
    11101 tcctcgatcc ggcggtcgat ctctcgaatg cggatttgcg agtcctgaat ggcccgatgg
    11161 acgtcggcca ggctcgaccc gatgagggtc aggatctcgc tccagtcctc gtgcggtggg
    11221 atctgtcccg ctggctgggg cgcgatgggc ctcgtcggca gctctgcaag ccgcgtcacg
    11281 aaggctttct gggtttccgc cgtttcgatg cgggcctcga agctcgcccg ctcgtccttc
    11341 aggcgctcga tttcattctc gatgcggcgc cgctccgtgc gctgcgcttc ggcgttcgcg
    11401 cgttccacga cgatgcgccg gctgtcgacg gcgccgatct cgagcttgcc cgtcgccttg
    11461 ccttcgacgc ggatcgagct ttcgaccgcc tgctgcggca gatcaccgat gacgaccgtg
    11521 tgatcaccgg cttcgagttg caggcgcacg acccggcgta cctcggcgcc ggacgggtat
    11581 accgtgacgg cttcgacacg ggaggtcacc ggcagctcgg ctgcccggag cggcagcacg
    11641 gccgcaagca acatcacaac gccgagcaga acaggcatcg gtctccctcc cagccgcatc
    11701 atctccgccg gaggcagacg ttaacgacgt tcatgtcgcc actgtgacgg ccgcaggggc
    11761 tcgggctcga tacttgcctc agaactcgag gcggtgggcc cgcgtcacca tcggcagcgc
    11821 gcgtaccttg gccaggattt cgtccgaaac cggctcgtcg agggcgacga ggcagatggc
    11881 atcgcccccc ggcctgtcgc ggccgagatt gagggtggcg atgttgaggc ccgcctcgcc
    11941 catcagcgac ccgaacttgc cgatgaaccc cggcttgtcc tggttggtga tgtagagcat
    12001 gtgcgggctg agctcagcct ccatgccgat gcctttgatc tggatgatcc gggggcggcc
    12061 atcggagaac acggttcccg ccacggagcg ctcgccgcgc tcggtctgga ccgtcaggcg
    12121 cgcataggtc aggtacgcgc cttcctcgcc ccgcgtgacc tcctcgacgg ccagaccacg
    12181 ctcacgcgcc attgccgggg cagagaccat gttgacctcg gtgagctgcg ggcgcagcag
    12241 gccggccagc atggcggccg tcagcgcgcg cgtgttcatc tccgcgatct cgccgatgta
    12301 ctcgatcctg acctgcttca ggcgcgtctc ggtgagctgg cccgcgaaca tgcccagctg
    12361 ctcggcgagc ttgatgaacg gcgtcagctt cggcgcctct tccgccgtga tgttcgggaa
    12421 gttgatcgcg ttcgagatgg cgcccttgag caggtagtcc gacatctgct cggcgacctg
    12481 cagcgcgacg ttctcctgtg cttccgtcgt agacgctccg agatgtggtg tgcagatgac
    12541 gttatccagt ccgaacaccg ggctttcacg cgcgggctcc acctcgaaca cgtcgagcgc
    12601 ggcgcccgcg acacggcccg ccttcaatgc ctccgccagt gccgcctcgt cgatcagtcc
    12661 accgcgggcg cagttgatga tgcgcacagt cggcttcatc ttcgacagcg ccttggcgtc
    12721 gatgatatgg cgcgtacgct ccgtcaacgg cgtatggagg gtaatgaaat cggcacgctg
    12781 gaaaagctca tcgagctcga ccttctccac gccgatctcg atcgcccgct ccggcgacag
    12841 gaacgggtcg taggcaatga cacgcatttt caggccgatg ccgcggtccg caacaatcga
    12901 gccgatgttg ccgcagccga tgatcccgag cgtttttcca aacaactcga cgcccatgaa
    12961 cttcgatttt tcccatttgc cggcttgagt ggatcgatcg gcctgcggga tctggcgggc
    13021 gagcgccagc atcagcgaga tcgcgtgctc cgcggtcgtg atcgagttgc cgtaaggcgt
    13081 gttcatcacg atgatgccac gggccgtcgc agcacgcgta tcgatattgt cgaccccgat
    13141 gccggcgcgg cctatgacct tcagccgctt gccggcggcg atgatcttct cggtgacctt
    13201 ggtggcggac cgtacggcga ggccgtcgta gtcgccgata atcttttcga gctgatcctt
    13261 agagaggccg accttcacat cgacatcgac accgcgcttc ttgaatacgg caatggcggc
    13321 cggagaaagc tcgtcggaaa tgagaacctt gggagtcatg aggagaaatc cttttggaat
    13381 ggttttgcgt cattccgact ttgatcgaaa cgtatcgcgg tgcactccca acctccctct
    13441 ccccggcggg gagagggtca gggtgagggg gttccgccag tggcgattct tgcaacccct
    13501 cacccggccc ttcggggccg acctcggatc aagtccgggg caggctctct ccccatggga
    13561 gaggtaaagg aagtgcctcc gacgccttca atcgcaatcg gagaagctct gcccgcagcg
    13621 caatcgctac cgggtgggcg gaatgccggc tgccgagcgc acccggcatg ctcaatggtt
    13681 caggccgcct tcgacagact ggcgcgcgcc gtcgcgaacg cccagtccag ccatggcagc
    13741 agcgcctcga cgtcggatcg ttcaacggtc gagccggtcc agatcctgag gcctggcggt
    13801 gcatcgcgat aagccgcgat atcgtgggca acgccctcct tgtcgagcag ggccgcaatc
    13861 tgtttggcaa aggcccattg ctgatcgacg gagagccgcg tcacggccgg atcgacaatg
    13921 ctgagacaca ccgacgtgtt cgaccgcgtg gccgggtcgc gggcaagatt ggcgatccac
    13981 ggcgtgcgct cgacccagtc gaagatcgc
//
LOCUS       DASVDU010000205        15891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_401467, whole genome shotgun sequence.
ACCESSION   DASVDU010000205 DASVDU010000000
VERSION     DASVDU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15891
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401467"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1613
                     /locus_tag="VF226_13140"
     CDS             237..1613
                     /locus_tag="VF226_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7074979.1"
                     /translation="MTAPRTNSKIEAEYRARTQRSAELYEQACQVIPAGLTHDSRTLL
                     PYPIYAARASGPRKWDVDGNEYVDYFGGHGALLLGHCHPAIAEAVTRQVTLGTHWGSS
                     HELEVRWAELVNRLIPCAERVRFTASGTEASHLALRLARAFTGKPKVIRFIGHFHGWH
                     DQVAAGSNSHYDGSVPAGVLPQIVEHTILLPADDPARVAETLSQRDDVAAIIFEPSGA
                     SWGQVPMPPGFIASLREITSQHGVLLIFDEVITGFRWSRGGAQARIGVTPDMCVLAKI
                     VAGGLPGGAVAGRADIMDQLDAAAAKRSAREKIGHQGTFNANPLCAAAAVATLSIVEK
                     EDVCERAERTAESIRSGMRQILIEEQIPWGVYGEASAFIIFQNPKKLPLDPATFDPLQ
                     YGFAELKAARNADLSHRLRIALLANGVDIMGAPGGLVSATHGEREVAQTLEAFRTAVR
                     WMKADGDV"
     gene            complement(1677..2186)
                     /locus_tag="VF226_13145"
     CDS             complement(1677..2186)
                     /locus_tag="VF226_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074980.1"
                     /translation="MTKRTVTVNDRMQKGYRYERTAPIGCDFDPEFRPDLTPPEMLRL
                     GVFGGKYMTDCRDEFPASWFEGAKLAAGRRDASLNYFGVDASQPLSEWRSKGWLHPDD
                     PRGWFQWYCRYYMGRRMQDEDARQIKRWKAIRRHVRQLQRSCEPGDLSCRRRQRQALL
                     HWAYDSRKL"
     gene            complement(2314..3405)
                     /gene="folP"
                     /locus_tag="VF226_13150"
     CDS             complement(2314..3405)
                     /gene="folP"
                     /locus_tag="VF226_13150"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7074981.1"
                     /translation="MNRDLYIRPVGLSAAPPAGEEEGEGAAGLTLAGGWLTFMGLEVI
                     ERVGRDRRRTRVFEIGEYCERDWGAHTAIAADLFEAVRRPRPRIAGLSLDRPRIMGIV
                     NITPDSFSDGGRLPTAQAAIDHALRLAEEGADILDLGAESTRPGSDAIPVEEELRRLM
                     PVLEGLAGRTGALISVDTRKAEVMRRAADAGADIINDISGLTHDPDALEAAAESGLPV
                     VLMHTLGDPKTMQVDPHYDDVVLDIFDYLEGRVKSAEAAGIDRSRLIVDYGIGFGKTL
                     EHNLSLLAQTALFHALGVPVLVGASRKRFIGTLTGIEQASERVAGSVGAALAAAGQGA
                     QIIRVHDVAATRQALTVWEAAVSGSAPRR"
     gene            complement(3568..5502)
                     /gene="ftsH"
                     /locus_tag="VF226_13155"
     CDS             complement(3568..5502)
                     /gene="ftsH"
                     /locus_tag="VF226_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7074982.1"
                     /translation="MNPHFRNFAIWVIIGLLLVALFNLFQNPAQSRRGNEIPYSDFLN
                     AVESRTVSEVLIQGNRISGKFSDNGIPFSTYAPEDPGLVERLHQQNIKIAARPSDEDV
                     PSFLSVLVSWFPMLLLIAVWVFFMRQMQSGSGRAMGFGKSKAKLLTERHGRVTFEDVA
                     GVEEAKSDLQEIVEFLRDPQKFQRLGGRIPRGALLVGPPGTGKTLLARAIAGEANVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDER
                     EQTLNQLLVEMDGFEANEGIILIAATNRPDVLDPALLRPGRFDRQIVVPNPDVTGREK
                     ILRVHMRKVPLAPDVDPKVIARGTPGFSGADLANLVNEAALLAARRNKRLVTQLEFED
                     AKDKVMMGAERRSMAMTEEEKLATAYHEAGHALVNLLMPGADPLHKVTIIPRGRALGV
                     TMSLPERDKLSYSKQWCEAKIAMCFGGRVAEQIIYGKEHLNTGASSDIMQATDLARRM
                     VTEWGMSEKLGPLRYNENQQEVFLGHSITQSQHMSEDTARMIDLEIRRIVTEGEKKAW
                     EILSSHREELEAITQALMEFETISGEECLTVMRGEKIVRRDDDENTKGPIGSAVPSAG
                     RIRPREEPDAPGGMEPQPQV"
     gene            complement(5712..7139)
                     /gene="tilS"
                     /locus_tag="VF226_13160"
     CDS             complement(5712..7139)
                     /gene="tilS"
                     /locus_tag="VF226_13160"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7074983.1"
                     /translation="MAATGPIGSAERDLLLARVRLPVAVAVSGGADSTALMHLVAGWA
                     AIQGRLEGLPDGVAPILVITVDHGLRPEAAAEAAWVAGEARRLGLAHVTLRWRGQKPR
                     TAIQETARAARYALICEHIGREPLPKPRQVLLAHHQDDQAETVLMRLARGSGVDGLSG
                     MREVEVRTWVHLKHPVEERLIAFVRPLLSVPKARLEATLQAIGAGWLRDPSNADQRYE
                     RVRLRAAVREREALGLTAEMLGLTAERIGSARAALERAHQELARTAVDLHDGAWASID
                     RQMLQRAPQEIALRLLQATIGAFGGQREPPSRLQMERLLERILARTTTAFTLGGAVVE
                     DIKSSKRRERPILAIYREPGRRPLPKATLWPGHGLFWDRRFYISLSPGYEHPVRVEAL
                     GQTGFARLKRAHRSLRELGIPAAAAAALPAIWAGDRLLVVPCLSAGEPGLLGPVAEDG
                     EPMVGASFAPQHIRTVLGRFGEDDI"
     gene            complement(7512..8534)
                     /gene="ybgF"
                     /locus_tag="VF226_13165"
     CDS             complement(7512..8534)
                     /gene="ybgF"
                     /locus_tag="VF226_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX7074984.1"
                     /translation="MGRWNHNLLGVAGALLLAAGSAHAEQKAMASASSAQQATKAAPF
                     QVAQARTKGTAARPSGAGADSDLRQRVEQLERQFLDMQVIIGTLESLARNSGTANPSM
                     TSAPGGIGSGADAARIDALETQVRALTAQLEQTSAQIRALGAGGTTRSIDSAPLPPSS
                     SAPVALDAAPSIDFGATVVKRAQGSDPIGQIISEETPTGFGSQVALAAPGAGGGDPKQ
                     DYETAYGYLLQQNYAAAERAFEDFLQRYPNDTLAGNAQYWLGESLYVRGQYKAAASAF
                     LKGYQNYSNSSKAPDSLLKLAMSLDKLGQKEAACSSFDELDARFPNAPSHVKTRSLTE
                     RQRVGC"
     gene            complement(8783..9292)
                     /gene="pal"
                     /locus_tag="VF226_13170"
     CDS             complement(8783..9292)
                     /gene="pal"
                     /locus_tag="VF226_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7074985.1"
                     /translation="MSQMETLKRLASLARVWAIVLLAVSAAGCANTAQEAGLGPGRGP
                     ATPGSQRDFVQNVGDIVYFSTDSSELTPEAQQTLNGQARWLRQYPQYTITIEGHADER
                     GTREYNIALGARRATAVRNYLVQNGVNANRIRTISYGKERPVAVCNDISCWSQNRRAQ
                     TVLNNRAGS"
     gene            complement(9779..9904)
                     /locus_tag="VF226_13175"
     CDS             complement(9779..9904)
                     /locus_tag="VF226_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074986.1"
                     /translation="MKGAIVVAALLTAVALGACRKEVPYEPMKLGGAVPAVEIAR"
     gene            complement(9945..10082)
                     /locus_tag="VF226_13180"
     CDS             complement(9945..10082)
                     /locus_tag="VF226_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074987.1"
                     /translation="MQGVKGAIVISTIIAAVALGACRREVPYEPMKLGADLPVAEKVA
                     R"
     gene            complement(10358..11617)
                     /gene="tolB"
                     /locus_tag="VF226_13185"
     CDS             complement(10358..11617)
                     /gene="tolB"
                     /locus_tag="VF226_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041803268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEX7074988.1"
                     /translation="MVAPMAFGQTRVTVDEANLRPRPIAIPDFLSEDPRLGAEIAGVV
                     AADLESSGLFRPLDRAAFIEQIRDINIAPRFADWRSIGAEGLVVGRVGRMGDGRLRAE
                     FRLWDTVLGKPLAGQQFSTIEANWRRIGHIIADQVYEKLTLEKGYFDTRIVFIDETGP
                     KDRRHKRLAIMDQDGANVRMLSDGRELVLTPRFSPTSQEITYMVYSGDQPRVMLMQLD
                     TGQRQIVGDFPGMTFAPRFSPDGQRIVMSFQIAESSSIVEMDLRSRQSRQLTRTTAID
                     TGPCYDPTGRYIVFESDRDGAQQLYVMNSDGSNVHRISYGEGRYSTPVWSPRGDYIAF
                     TKQYAGEFLIGVLKPDGSGERILTEGFHNEGPTWAPNGRVLMFFRDHPGPRGGPRIYS
                     IDLTGYNERLISTPSFASDPAWSPLLS"
     gene            complement(11745..12833)
                     /gene="tolA"
                     /locus_tag="VF226_13190"
     CDS             complement(11745..12833)
                     /gene="tolA"
                     /locus_tag="VF226_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEX7074989.1"
                     /translation="MQLGLTFSILMHLGVLAWAFITLQSPKPFNVPEPEPVEVALVTP
                     EDLVRLTKGDRNAKQLEAQQAENQQEAKKEKPQRREATAPPPSAPPPPPPEETADPEP
                     PKAEPRRDPIAEKLAALPPEPAGPTPEEIKQKEAEAAALKAEEARKAEEARKAEEEKK
                     RAEAEAKKRAEAEAKKKAEEAKKRAEEKKKQEERRKRLAEKKRKEQEAKKKKEFDANR
                     IAALLNKVPDTAPRAGAPEPPLTQTNARGPVAGAPEGRDSRLSASQRSLLGVMMKRAV
                     SRCWNINSGLDGIDRIVVEVEVRLRPDGHLEGTPRVINSGSGALFADAANSAIRALVQ
                     CEPYDLPQEFYVGGWDHMVVTFDPQRMF"
     gene            complement(12841..13308)
                     /gene="tolR"
                     /locus_tag="VF226_13195"
     CDS             complement(12841..13308)
                     /gene="tolR"
                     /locus_tag="VF226_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEX7074990.1"
                     /translation="MGASINIGNGGGGRRRRVRSRVPMSEINVTPFVDVMLVLLIIFM
                     VAAPLLTSGVPLELPQAKGKQLEAQDKEPVVVSVTSDGKLFLGQEDKTPIPLDELGPK
                     LQAVAEARGGMDEPIFVRGDRAVEYGTVARVMARIKEAGFRKLSLVTEVESGG"
     gene            complement(13317..14018)
                     /gene="tolQ"
                     /locus_tag="VF226_13200"
     CDS             complement(13317..14018)
                     /gene="tolQ"
                     /locus_tag="VF226_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEX7074991.1"
                     /translation="MLEGTLAPNVGGGVSFLELFLQAHIVVKIVMIGLLLASVWSWAI
                     IFEKLVAFRRARVEADRFEQLFWSGQSLEELYAGLSRGPTIAIAALFVAAMREWKRSV
                     EGNVRALGGIQLRVEKVMDVTITREMERLDRRLLFLATVGSTAPFIGLFGTVWGIMTS
                     FQAIAASKNTSLAVVAPGIAEALFATALGLLAAIPAVVFYNKFSADSTRLGQRLEAFA
                     DEFSAIVSRQIDVRS"
     gene            complement(14393..14863)
                     /gene="ybgC"
                     /locus_tag="VF226_13205"
     CDS             complement(14393..14863)
                     /gene="ybgC"
                     /locus_tag="VF226_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEX7074992.1"
                     /translation="MSGAKRDWPDLSGRIVEDDGGRRHVLPVRVYFEDTDFSGVVYHA
                     SYLRWCERGRSDFLRLLGNDHQALMAGASGREPAAFVVRRMSLEFLKPARIDEVLEVN
                     TRVREIGAASLTLSQHITRDDVMLFEAEVLIVLVSLSGKPMRISQAIRSMLGSG"
     gene            complement(14955..>15891)
                     /locus_tag="VF226_13210"
     CDS             complement(14955..>15891)
                     /locus_tag="VF226_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074993.1"
                     /translation="FPESGIGGAVERALRAALGEGHAHRTRIQRAGVGIAATAILSTL
                     LLCGSQFILPTSLEPDAAESRLSDVSHASSSQWAFASAAFAAATESETQEDSDLGMGV
                     PAFAALGQKVLRDEEGDISRPPTVVTLAARETVAARPGETVDLPFEVKGADALAAGTT
                     LVIQGVPENAALSAAEPQGDGVWTVPIEQAADVKLTAYALSTGTSENLVAELRSPEGE
                     VLARTTTRLLASGPQVPGAAVAESRPGDKPVSGSEARPASATVSEWATVAVVPKSSKA
                     AAEQRTETVARSDESPREAPEWLTDGSRSALGGPR"
BASE COUNT         2727 a         5329 c         5015 g         2820 t
ORIGIN      
        1 tagaggcgtg gttgcggtcg gtgccgccga tgatgccaaa ccaccggctg acgcgagatg
       61 agaccagtgc tcttgcagct ttcatcatgt cgctgcgcaa agcgccgtga acagcattcg
      121 cagtcgagag ttcaggttcg agaccaagcc cttgagacag gacgagactt ccgttgtagc
      181 ctcgcccgga gagtgcttag acagtctgag ccacgacaga agcgagatca gcgatcatga
      241 cggcacccag gaccaactcg aagatcgaag ccgagtaccg tgcccgcacg caacggtcag
      301 cggaactcta cgagcaggcc tgccaggtga tcccggcggg cctgacgcat gattcccgca
      361 cgcttctgcc ctatccgatc tatgccgcac gcgcgagcgg cccccgcaaa tgggacgtcg
      421 acggtaacga gtacgtcgac tatttcggcg gtcatggcgc cctgctcctc ggccactgtc
      481 atccggccat cgccgaggct gtgaccaggc aggtgaccct cggcacgcac tgggggtcct
      541 cgcacgagct ggaggtgcgc tgggctgagc tcgtgaaccg gctcattccc tgcgcggaac
      601 gggtgaggtt caccgcttca ggcaccgagg catcccatct ggcgctcaga ctcgcccgcg
      661 cattcaccgg caagcccaag gtgatccgct tcatcggcca cttccatggc tggcacgatc
      721 aggtcgctgc cggctccaac tcccactacg atgggagcgt gccggccggc gttctgccgc
      781 aaatcgtcga gcatacgatc ctgctccccg cagatgatcc tgcgcgtgtt gccgagacac
      841 tcagccaacg tgacgacgtt gccgccatca ttttcgagcc gtccggcgca tcgtgggggc
      901 aagtgccaat gccgccaggc ttcatcgcca gcctacggga gatcactagc cagcacggtg
      961 tgttgctcat cttcgacgag gtcatcaccg ggttccgctg gtcccgcggc ggggcgcagg
     1021 cacgcatcgg cgtgacaccc gacatgtgcg tgctggcgaa gatcgtcgcc ggcgggctgc
     1081 ccggaggcgc cgtcgcaggg cgcgccgaca tcatggacca gctcgacgca gccgccgcca
     1141 agcgcagcgc gcgtgagaag atcggccatc agggcacgtt caatgccaac ccgctctgcg
     1201 ccgctgcggc cgtcgcgacc ctctccatcg tggaaaagga ggacgtctgc gagcgcgccg
     1261 agcgcaccgc cgagtccatc cgttccggga tgcgccagat tctgatcgaa gagcagattc
     1321 cgtggggcgt ttacggcgag gcgtctgcgt tcatcatctt ccagaacccg aaaaagctgc
     1381 cgctggaccc ggcgaccttc gatccccttc agtacggctt tgcggagctc aaagccgccc
     1441 gcaacgccga cctttctcac cggctccgca tcgcactgct ggccaacggg gtcgacatca
     1501 tgggcgcgcc gggcgggctc gtatccgcga cgcacggcga gcgcgaggtg gcgcagacac
     1561 tcgaggcctt ccggacggcg gtgcgctgga tgaaggcgga cggcgatgtt tgaactcgcg
     1621 ccaggtcgag attgctttag gccgctatcg cggtgatcct cgccggccga ctccgcttac
     1681 agcttgcggc tgtcataagc ccagtggagg agagcttggc gttgccgtcg ccggcatgag
     1741 agatcacccg gctcgcagct gcgttgcagc tggcgcacat ggcgccggat agccttccac
     1801 cgcttgatct gacgggcatc ttcgtcctgc atccggcgcc ccatgtaata gcggcagtac
     1861 cactggaacc aaccgcgcgg atcatcagga tggagccagc ccttgctgcg ccactcggag
     1921 agcggctggc tggcatcgac accgaaatag ttgagcgaag catcgcggcg cccggccgcg
     1981 agcttggctc cttcgaacca gcttgcgggg aactcgtcgc ggcaatccgt catgtacttg
     2041 ccgccgaata ccccgagccg aagcatctcg ggtggcgtga ggtcgggacg gaactcggga
     2101 tcgaagtcgc acccgatcgg ggcagtacgc tcgtaccgat agcccttctg catccggtca
     2161 ttgactgtga cggttcgctt cgtcactgcg cttgggccct tagcttgagg cgccgctggc
     2221 agggaaacgc ctaatcaagc ccttcggtgc gcgcgacaga aatccgctcc gtgactcgat
     2281 cggtggctca gcggagtagc cggtgacgaa acctcagcga cggggcgcgc tcccgctgac
     2341 ggccgcctcc cacaccgtca gcgcctgacg ggtggcggcg acgtcatgca cgcggatgat
     2401 ctgagcgcct tgccccgcag cggcgagagc cgcgccgacc gaaccggcca cgcgctccga
     2461 tgcttgctca atgccggtca gcgttccgat gaagcgcttg cgcgaggcgc cgacgaggac
     2521 cggaaccccg agcgcatgaa acagcgccgt ctgcgccagc agggaaagat tgtgctccag
     2581 cgtcttgccg aagcctatac cgtagtcgac gatcagcctg gagcgatcga tgcccgccgc
     2641 ctctgcgctt ttcacccgtc cttccaagta gtcgaagatg tcgagcacca cgtcatcgta
     2701 gtgggggtcc acctgcatgg tcttgggatc gccgagggta tgcatgagca ccaccggcag
     2761 gccgctctcg gcggcagcct ccaaggcgtc cggatcgtgc gtgaggcccg agatatcgtt
     2821 gatgatatcc gctcccgcat cggcggcccg ccgcatcacc tcggccttgc gcgtgtcgac
     2881 cgagatcagc gccccggtgc gaccggccaa gccttcgagc accggcatga ggcggcgcaa
     2941 ttcctcctcg accggaatgg catccgaacc cggccgcgtc gactcggcgc caaggtcgag
     3001 gatgtccgcc ccttcctccg cgagccgcag ggcgtggtcg atggcggctt gcgctgtcgg
     3061 cagccggcca ccgtcggaga agctgtccgg ggtgatattg acgatgccca tgatccgcgg
     3121 ccggtcgagc gagagccccg caatgcgggg gcggggcctg cgcacggctt cgaagagatc
     3181 ggctgcgatg gcggtgtggg cgccccagtc ccgctcgcag tactcgccga tctcgaacac
     3241 ccgcgtgcgg cggcggtccc ggccgacgcg ctcgatgacc tccagtccca tgaacgtgag
     3301 ccagccgccg gcgagcgtaa ggcccgccgc gccttcgcct tcctcttcac ctgcaggtgg
     3361 agcggcggag aggccgacgg gtcggatgta gaggtcgcgg ttcatttgcc gggtgaaatc
     3421 gtgaaatggt gaattcgtga agtcgcaagt cagctggtcg ccagaggata ccccaccatt
     3481 tcacgacttg gcgacttcac gatctcacgc aacaaaaaag cggctgcaga ttgctccgca
     3541 gccgctccac ttgcgttccc ctcaacctta cacctgcggc tgaggctcca ttccgcccgg
     3601 ggcatccggt tcctcgcgcg gccggatgcg gccggccgac ggcaccgcgg agccgatcgg
     3661 acccttggtg ttctcgtcgt cgtcgcgacg gacgatcttc tcgccgcgca tgacggtcag
     3721 gcactcctcg ccactgatcg tctcgaactc catcagcgcc tgagtgattg cctcgagctc
     3781 ctcgcggtgc gagctgagga tctcccaggc cttcttctcg ccttccgtga cgatgcggcg
     3841 gatctccagg tcgatcatcc tggccgtgtc ttccgacata tgctggctct gcgtgatcga
     3901 atggccgagg aagacctcct gctggttctc gttgtaacgc agcggaccga gcttctcgct
     3961 catgccccac tcggtcacca tccggcgcgc caggtcggtc gcctgcatga tgtcgctgga
     4021 ggcaccggtg ttcaggtgct ccttgccgta gatgatctgc tcggcgacgc ggccaccgaa
     4081 gcacatcgcg atcttcgcct cacaccactg cttcgagtag ctgagcttgt cgcgctccgg
     4141 caggctcatg gtgacaccga gcgcgcggcc gcgcggaatg atggtcacct tgtgcagcgg
     4201 atcggccccc ggcatcagca ggttgaccag cgcgtggcct gcctcgtggt aggccgtcgc
     4261 cagcttttcc tcttcggtca tggccatgga ccggcgctcg gcgcccatca tgaccttgtc
     4321 cttggcgtcc tcgaactcga gctgcgtgac gagccgcttg ttgcgacggg ccgcgagcag
     4381 ggccgcctcg ttcacgaggt tggcgaggtc ggcgccggag aagcccggcg taccccgtgc
     4441 gatcaccttc gggtcgacgt ccggcgccag cggcaccttg cgcatgtgca cgcgcaggat
     4501 cttctcgcgg cccgtgacgt cggggttcgg cacaacgatt tgccggtcga agcggcccgg
     4561 gcgcagcagc gccggatcga gcacgtccgg cctgttcgtg gccgcgatga ggatgatgcc
     4621 ctcgttggcc tcgaagccgt ccatctcgac gagcagctgg ttcagcgtct gctcgcgctc
     4681 gtcattgcca ccgcccaggc ctgcgccacg gtgacggccg acggcgtcga tctcgtcgat
     4741 aaagatgatg cagggcgcgt tcttcttcgc ctgctcgaac atgtcgcgca cacggctggc
     4801 gcccacgccg acgaacatct cgacgaagtc ggagccggag atggtgaaga acggcacgtt
     4861 cgcctcgccg gcgatcgcgc gggcgagcag cgtcttgccg gtgcccggcg ggccgacgag
     4921 cagcgcaccg cgcggaatcc gaccgccgag gcgctggaac ttctgcggat cacgcaggaa
     4981 ctcgacgatc tcctgcaagt cggacttggc ctcttcgacc cctgccacgt cctcgaaggt
     5041 cacgcgccca tggcgttcgg taagcaattt ggccttggac tttccgaatc ccatggcccg
     5101 gcccgatccg gactgcatct ggcgcatgaa gaagacccag accgcaatca gcaggagcat
     5161 cgggaaccag gagacgagca cgctgaggaa cgacggcacg tcctcgtccg acggccgcgc
     5221 ggcgatcttg atgttctgct ggtgcaggcg ctcgacgagc cctgggtcct ccggcgcgta
     5281 cgtgctgaag gggatgccgt tgtcgctgaa cttgccggag atacggttgc cctggatcag
     5341 gacctccgag accgtccgcg actcgaccgc gttcaggaag tcggaataag gaatttcatt
     5401 cccccggcgg ctctgcgccg ggttctggaa caggttgaac agtgcgacga gcaacaggcc
     5461 gatgatgacc caaatcgcaa agttccggaa gtgcggattc atcttgttcc cttctggagg
     5521 aggctcgaga gagccgaccg ccgcgcgcca catggctgcg cagcgctcat gaccccgaat
     5581 gggctagaca taggtgcatg cagaacgatt gccaagttgc cgctggcagc ttcgcgccaa
     5641 tcccagaaca cctcttccag ctcgatcgct tttctaacat gaattatggc agaaagccta
     5701 acggggtggg ctcaaatgtc gtcctccccg aaacggccga ggaccgtgcg gatgtgctgg
     5761 ggcgcgaagg aggcgccgac catgggttcg ccgtcctcgg cgacgggacc gagcaggccc
     5821 ggctcgccag ccgatagaca aggcacgacc agcagccgat cgcctgccca gatcgccggc
     5881 aacgcggcag cggcagcggc cggaataccg agttcacgca ggctccgatg tgcccgcttg
     5941 aggcgggcga aaccggtctg gcccagcgcc tcgacgcgga ccgggtgttc gtatcctgga
     6001 gacaggctga tgtagaagcg ccggtcccag aacaagccgt gccctggcca gagcgtggcc
     6061 tttggcaacg gccgccggcc cggctcgcgg tagatggcca agatcggtcg ctcccgtcgc
     6121 ttcgaagact tgatgtcttc gacgaccgcg ccacccaacg tgaaggctgt tgtagtgcgc
     6181 gcgagaatgc gctcgagcag ccgttccatt tgcagccggc tcggcggctc ccgttgcccg
     6241 ccaaacgcgc cgatggtcgc ctgcagcaga cgcagcgcga tctcctgcgg cgcccgttgg
     6301 agcatctgcc tgtcgatgct ggcccatgca ccgtcgtgaa ggtcgactgc agtgcgcgcg
     6361 agctcttgat gtgctctctc cagggccgcg cgggcggagc cgatccgctc tgccgtcagc
     6421 cccaacatct cggcggtgag gccgagcgcc tcgcgctcgc ggacggccgc tcgcaggcga
     6481 acgcgctcgt agcgctggtc ggcgttgctc gggtcgcgca gccaaccggc cccaattgcc
     6541 tggagcgtcg cttcaagtcg cgccttcggg acgctcagca acgggcggac gaacgcgatc
     6601 aggcgctcct ccacggggtg cttgaggtgc acccaggtgc gcacctcgac ctcccgcatg
     6661 ccggataacc cgtcgacacc actgccgcga gccagtcgca tcagcaccgt ttcggcttga
     6721 tcgtcctggt gatgggcgag gaggacctga cgcggcttcg gcaagggctc gcggccgata
     6781 tgctcgcata tcagagcgta gcgggccgcc cgtgccgtct cctgaatggc cgtccgcggc
     6841 ttctggcccc gccagcgcaa ggtcacatga gcaagaccga gccgccgtgc ttcccccgcg
     6901 acccacgcgg cttcagcggc ggcctccggc cgcaacccgt gatcgaccgt gatgacgaga
     6961 attggggcaa cgccgtccgg caatccctca agcctgcctt gtatggcagc ccagccggcc
     7021 accaggtgca tcagtgcggt gctgtccgca ccaccggaga cggcgactgc aacgggcagg
     7081 cgcacgcggg ccaggaggag gtctctttcc gcggaaccga tcggccccgt tgccgccacg
     7141 acgtctagtt cctcgagttc gaagctcgcc caccttcgag gccgggtccg tcggcgagct
     7201 tcgaaatcgt gaaggaaaca agacccagag agtcgctgga gaagctgaac tcatggccac
     7261 tttccctctc cccagcggag agagggctag ggtgaggggg ttccgccggt cgcgatcgtt
     7321 gcaacccttc acccggccat tcgggccgac cccggatcaa gtccgaggca ggctctctcc
     7381 ccacaggaga ggtgaagggc gtgattgttg ccgtcgtttc cacctgaatc gggagagctt
     7441 cagtgtgctt tggatcgaga aagccgctcg aagccttgcc gtcagagcaa gcagatttct
     7501 cgatcatcac actagcagcc gacgcgctgg cgctcggtga gggagcgcgt cttgacgtgt
     7561 gagggcgcgt ttgggaaccg ggcgtccagc tcgtcgaagg acgagcacgc ggcctctttc
     7621 tggccgagct tgtcgagcga catggcgagc ttgagcaagc tgtccggcgc cttcgaactg
     7681 ttactgtagt tctgataccc cttcaaaaac gcgctggcag ccgccttgta ctggccgcgg
     7741 acgtagagcg actcgcccag ccagtactgg gcatttccgg caagcgtgtc gttcgggtag
     7801 cgctgcagga agtcctcgaa cgccctttcg gctgccgcat agttctgctg caaaagatag
     7861 ccgtacgccg tctcgtaatc ctgcttggga tctccgccgc cagccccggg agcggccagc
     7921 gcgacctggg agccgaagcc ggtaggcgtc tcctcgctga tgatctggcc gatcggatcg
     7981 ctgccttggg cccgcttcac gacggtcgct ccgaaatcga tgctcggcgc ggcatcgagc
     8041 gcgacaggcg ccgatgagga cggcggcaat ggcgcgctgt caatcgaccg ggtcgtgccg
     8101 ccagcgccaa gggcccggat ctgcgcggac gtctgctcga gctgagcggt cagcgcacgg
     8161 acctgcgtct cgagagcatc gatccgggcc gcatcggccc ctgagccaat cccgcctgga
     8221 gccgaggtca tggaggggtt ggcggtacca gagttccggg cgagcgactc gagcgtgccg
     8281 atgatgactt gcatgtcgag gaattgccgc tcgagctgct ccacccgttg gcggaggtcg
     8341 gaatcggcgc ctgcaccact cggtcgcgcc gcagttccct tcgtgcgtgc ctgcgcgacc
     8401 tggaatggtg ccgctttcgt cgcttgctgg gcggagctgg cggaggccat tgccttctgc
     8461 tccgcgtgcg ccgaccctgc cgccagaagg agcgcgccag ccacgcccag cagattgtgg
     8521 ttccaacgac ccatcgaacg tgtcctgctc ttccccgatc gccaccctgt cggaccgccc
     8581 gaggcgagag cgatcatcag ccgcaatgcc gcgctccgac cgccggcgat gtcaactggc
     8641 cccaaatgac ggaggagccc ccgctcctca tttcagctgt tgatgctcca ttcgagcatc
     8701 ggtccggaaa cgatttcagc caatactggg gcgaaacttt gatcggatac gactagccgc
     8761 agccggtttg ttgcgccgcg cgtcagctcc ccgcgcgatt gttgagcacc gtctgcgcgc
     8821 ggcggttctg agaccagcag gagatgtcgt tgcagaccgc gaccggccgc tccttgccgt
     8881 acgagatggt ccggatgcga ttggcattga ccccgttctg gaccagatag ttgcgcaccg
     8941 ccgtggcccg ccgcgcaccg agcgcgatgt tgtactcgcg cgtgccccgc tcatcggcgt
     9001 gaccctcgat ggtgatggta tactggggat actgccgcag ccagcgcgcc tgcccattga
     9061 gcgtctgctg cgcctccggc gtcaactccg agctgtccgt ggagaaataa acgatatcgc
     9121 ccacgttctg gacgaagtct cgctgcgaac ccggcgttgc cggaccgcgg ccaggaccta
     9181 gtcctgcttc ctgggccgtg ttggcgcatc cggcagccga gaccgcgagc agcacaatgg
     9241 cccacacgcg tgccaagctc gccaatcgct tcaaggtctc catctggctg atcccttccg
     9301 ttgctgttcc ggccgcttgc gcacgaaata tcagtgatcg gaccggcaac aaacccgcgc
     9361 cccttatcgc cggacagggt tagcgccgtc gtcgtgcaaa aatgtggctg gcatcaggtt
     9421 cttgaaaaca atgcgaggac cgatggcaac atgcatcggt gctctgccca caactttgag
     9481 gcaaggcctc ggctgaggcg ccgcgcaggc ttcctacgtg ctccttggcg ctcccaaaac
     9541 ggaacgactg cgcttctccc tccgaccttg acgaaatttc gccgaacgca gctggctcga
     9601 tcgcagctgg cggccattgg ccatatcgga tcaaaagcac gcgagggctg agagggatca
     9661 gacggcgcag caaagcatga tcacaatgga caaagaccgt accactagcg ccagagggcc
     9721 agtggtacgg cctttgtctc gtactgccgc aacaagctgc agcagatacc acccacgatt
     9781 agcgagcgat ctccaccgca gggacggcac cgccgagctt catcggctcg tagggaactt
     9841 ccttacggca cgcccccaga gcaaccgcgg taagcagagc cgcgacaacg atagcgccct
     9901 tcatgttcat tctcctgatg atgttacagg cccccaccga atttctagcg agcgaccttc
     9961 tcggccacag gcagatccgc accgagcttc atcggctcgt acggaacttc acggcggcac
    10021 gctcccagcg ccacagccgc gatgatcgtc gaaataacga tcgcgccctt tacaccctgc
    10081 atggtcatct cctaatggct tcggcagtcg ccgaatattg gggtaccggt gtggagtacg
    10141 cagatcaagg acggagggaa ccacaggcta gttttccgcc cttgttgatg cgattatgcc
    10201 actccgtgtg acttggctcc cagcccttac actcaacgag gacttgcgta aggactgagc
    10261 atttcttgcg aagctcccga cggcacgcag cggagcatcc agaacatcaa aacatcgaat
    10321 cgcttaccga ttcgtaaacc cgcgtgcaat ttccggctca actcaggagc ggagaccagg
    10381 ccgggtcgga cgcgaacgag ggtgtggaaa tcaaccgttc gttgtagccc gtcaaatcga
    10441 tggagtagat tcgcggcccg ccccgagggc cgggatggtc ccggaagaac atcaggacac
    10501 ggccgttcgg cgcccaggtc ggcccctcgt tatgaaagcc ctcggtcaga attcgctcgc
    10561 cggatccgtc gggcttcagt acgccgatca ggaattcgcc ggcgtactgc ttggtgaagg
    10621 cgatgtagtc accgcgcggc gaccagaccg gcgtcgaata gcgaccctcg ccgtagctaa
    10681 tgcggtgcac gttcgatccg tcgctgttca tgacgtagag ctgctgcgca ccgtcccggt
    10741 ccgattcgaa cacgatgtag cgccccgtcg gatcgtagca ggggccggta tcgatcgccg
    10801 tggtgcgggt gagctggcgc gactggcgcg agcgcaggtc catctccacg atgctcgagg
    10861 actcggcgat ttggaagctc atgacgatcc gctgcccgtc cggtgagaac cgcggcgcga
    10921 aggtcatgcc tgggaaatcc cccacgattt gccgctggcc ggtgtccagc tgcatcagca
    10981 tcacgcgcgg ctgatcgcca ctgtagacca tatacgtaat ttcctggctg gtcggactga
    11041 accgcggggt caggacaagc tcccggccgt cgctcagcat tcggacattg gcgccgtcct
    11101 ggtccatgat cgccagccgc ttgtgccggc gatcctttgg gccggtctcg tcgatgaaga
    11161 cgatgcgcgt gtcgaaataa cccttctcga gtgtcagttt ctcgtagacc tgatcggcga
    11221 tgatgtgacc gatgcgccgc cagttggcct cgatcgtcga gaactgctgg ccggcgagcg
    11281 gctttccgag caccgtgtcc cagagccgga actcggctct gagccggcca tctcccatcc
    11341 ggccaacccg gcccacgacg agcccttcgg cgccgatcga acgccagtcg gcaaaccgcg
    11401 gcgcaatatt gatgtcgcgg atctgctcga tgaacgctgc gcggtcgagt ggccggaaca
    11461 gacccgagga ctcgaggtcc gcagccacca cgcccgcaat ctcggctccc agccgcgggt
    11521 cctcgctgag gaaatccggg atggcgatcg gccgtggccg gaggttcgcc tcgtcgaccg
    11581 tgacgcgggt ctggccgaag gccattggcg ccaccatggc gccggcgccc agcaggcccg
    11641 cctgcaatgc tgcgcgtcgc gtgatgccgc gattggtctt tcgtctggtc atcgctcagt
    11701 cctgtcgtcc atcgtcggct gcggctgctc gggttgtact ctcatcagaa cattcgctgc
    11761 gggtcgaatg tgaccaccat gtggtcccaa ccgcccacgt agaattcctg aggcaaatca
    11821 tacggctcgc actgcacgag agcccggatg gcgctgttcg cggcatcggc gaacaaggca
    11881 cccgaccccg aattgatgac gcggggagtc ccctccaggt gcccatcagg gcgcaaacgc
    11941 acctcgacct cgaccacaat tcggtcgatg ccgtcgaggc cgctgttgat gttccagcag
    12001 cgactgacgg cgcgtttcat catcacgccg agcagcgatc gctgactggc ggagagccgg
    12061 ctgtcacgcc cttccggcgc acccgcgacc gggccacggg cgttcgtctg cgtgagcggc
    12121 ggctccggcg cgccggcacg gggggcggtg tccggcacct tgttgagcag cgccgcgatg
    12181 cggttggcgt cgaactcttt cttcttcttg gcttcctgtt ccttgcgctt cttctcggcc
    12241 agccgcttcc gccgctcttc ctgcttcttc ttttcctctg cgcgcttttt cgcttcttcg
    12301 gccttcttct tggcttcagc ttcagctcgt ttcttggctt cggcttcggc gcgcttcttc
    12361 tcctcctcgg ccttgcgcgc ctcctcggct ttgcgtgcct cttcggcctt cagcgccgcc
    12421 gcttcagctt ccttctgctt gatctcctcc ggcgtcggac ctgccggctc gggcggcaaa
    12481 gcggcgagct tctccgcaat cggatcgcgc ctgggctcgg ccttgggcgg ctcaggatcg
    12541 gcagtctcct ccggcggcgg aggtggcggg gctgacggag gcggcgccgt cgcctccctt
    12601 cgctgcggct tctccttttt cgcctcctgc tggttctcag cctgctgcgc ctcgagctgc
    12661 ttggcgttgc ggtcgccctt cgtgaggcgg accaggtcct caggcgtcac cagggccact
    12721 tcgacgggct cgggctcggg cacgttgaag ggcttcggcg actgcagcgt gatgaacgcc
    12781 caggcaagga cgccgagatg catcaggatc gagaatgtga gacccagttg caccgctgcg
    12841 tcaccctccg ctttccacct ctgtgacgag tgagagcttg cggaagccag cttccttgat
    12901 gcgggccatg acgcgcgcga ccgtgccgta ttccaccgcc cgatccccgc gaacgaagat
    12961 cggctcgtcc atgccgccgc gcgcctcggc aacggcctgc agcttcgggc ccagctcgtc
    13021 gagcggaatg ggcgtcttat cctcctggcc gaggaacagc ttcccgtcgc tggtcacgga
    13081 caccaccacg ggctccttgt cctgggcctc gagctgcttg cccttggcct ggggcagctc
    13141 caacggcacc ccggacgtca gcagcggcgc cgcgaccatg aagatgatca gcaggacgag
    13201 catcacgtcg acgaacggcg tgacgttgat ctcgctcatc ggcacccgcg agcgcacgcg
    13261 ccgcctgcgt ccaccgccgc cattgccgat attgatgctg gctcccatgg ctccgctcag
    13321 ctccgcacgt cgatctgtcg cgaaacgatg gccgagaatt catcagcgaa ggcctcgagc
    13381 cgctgcccga ggcgagtcga atcagcagag aacttattgt agaagactac ggcaggaatt
    13441 gctgcgagga ggcccagcgc ggtggcaaaa agcgcctcgg caatacctgg cgccaccact
    13501 gcgaggctcg tgttcttcga ggcagcgatg gcctggaaac tcgtcatgat gccccagacg
    13561 gtgccgaaca ggccgatgaa aggtgccgta gatcccaccg tcgcgaggaa cagaaggcgc
    13621 cggtcgagcc gctccatctc gcgggtgatg gtcacgtcca tcaccttctc gacgcggagc
    13681 tgaatgccgc ccagagcgcg gacatttccc tcgacggagc gcttccactc gcgcatggcg
    13741 gcgacgaaga gcgccgcaat ggcgatggtc gggccgcggg agaggcccgc ataaagctcc
    13801 tcgagcgact ggcccgacca gaaaagctgc tcgaagcggt ccgcttcgac gcgggcccgg
    13861 cggaaggcga caagcttctc gaaaatgatg gcccaggacc acacggaggc aagcaacagc
    13921 cctatcatca cgatcttgac gacgatatgg gcctgaagaa acagctccag aaacgagaca
    13981 ccgccgccaa cgttcggcgc caatgtgcct tccaacatca gttaagcctc ataagtatct
    14041 gcaaacgagc cgtttcgagt tgccaacagc aaacgggccg gcacacgtcc gcgcccgccc
    14101 gttcgcgacg gcgatcttct tcgtgcaccg cccctggccc agagggccgc atgtgctctg
    14161 ccaatatgac caaactacgg cccgacgctg cgacgcggcg ctgccactcc cctgattcgg
    14221 aatggtatgc cagcctaccg accgtttcca ggcacgggga cagtgcacgc ccatttcgct
    14281 ggccttgcgc ggccctcctc ttacggaggg ccgattgttc atattgttcc tatactgaca
    14341 agcgccccga gcggcagttg tactgctttt ctaagagaag ttcgagccga agtcagccgc
    14401 tccccagcat ggagcggatc gcctgggaga tgcgcatcgg cttgccggac aggctgacca
    14461 gcacgatcag aacctcggct tcgaagagca tcacgtcatc gcgggtgatg tgctgagata
    14521 acgtcaggct ggcggcaccg atctcacgga cacgcgtgtt gacctcgagg acctcatcga
    14581 tccgggccgg cttcaggaat tcgaggctca tgcggcgcac gacgaaggcc gccggctcgc
    14641 ggccggaagc gccagccatc agcgcctggt gatcgttccc caatagccgc aggaagtctg
    14701 accggccgcg ctcgcaccag cgcagatagc tggcgtgata gacgacgccg gagaaatccg
    14761 tgtcctcgaa gtagacacgg accggcagca cgtgacgccg cccgccatcg tcctcgacga
    14821 tgcggccgga aagatcaggc cagtcccgct tcgctccgct cataggctct cgccgccacc
    14881 aaatcgcgcg ggtcgtgatc gcccccgcgc gcggagccgc ttctagcaca cattagccgg
    14941 atggacaggt gaactcagcg cggaccgccg agtgcggagc ggctgccgtc tgtcagccac
    15001 tccggagcct ctcgtggcga ctcgtccgac cgcgccacgg tttcggtccg ctgctcagca
    15061 gcagccttgg aggatttcgg cacgacggcc acggtcgccc actccgagac ggtcgcggaa
    15121 gcgggccgag cttccgagcc ggaaactggt ttgtcacctg ggcggctctc ggcgaccgcc
    15181 gccccgggca cctgcggccc ggacgccagc agtcgcgtcg tggtgcgcgc cagaacttca
    15241 ccctcgggcg agcgcagctc ggcgacgagg ttctcgctcg tcccggtcga aagggcgtag
    15301 gcggtgagct tcacgtccgc ggcctgctca atgggtacgg tccagacccc atccccctgt
    15361 ggctcggctg cggagagtgc cgcattttcc ggaacgcctt ggatcacgag cgtcgtgccg
    15421 gcagcaagtg catcggcccc cttgacctcg aacggaagat cgaccgtctc cccggggcgt
    15481 gccgcaaccg tttccctggc ggcgagggtg accaccgtgg gcggccgcga gatgtcgccc
    15541 tcctcgtcgc ggagcacctt ctgtccgagc gctgcgaacg ccggcacgcc catgcccaga
    15601 tctgagtcct cttgggtctc ggattcggtt gcggcggcga aggctgccga agcgaaggcc
    15661 cattggctgc tcgaagcatg cgaaacgtcg ctaaggcgcg attccgccgc gtcgggttcc
    15721 agggaagtcg gcagaatgaa ttgtgaaccg cacagcagga gggtcgacaa gatcgctgta
    15781 gcagcgattc caacgccagc gcgctgaatg cgcgtccggt gcgcatgtcc ctcgccgagc
    15841 gccgctcgca aggctctctc tacggctccg ccgatcccgg attccggaaa g
//
LOCUS       DASVDU010000206         4289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1608671, whole genome shotgun sequence.
ACCESSION   DASVDU010000206 DASVDU010000000
VERSION     DASVDU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608671"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF226_13215"
     CDS             <1..328
                     /locus_tag="VF226_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074994.1"
                     /translation="VARLFPAATASAAAARLWAYPAPERRAESAIDSEPCEIPSMMRQ
                     GFAATLTSYDAPSFTMNKIPAFDLKDSKFHKNERYQGGSFLFPILLTNLEAASSIAID
                     EGNWLT"
     gene            563..706
                     /locus_tag="VF226_13220"
     CDS             563..706
                     /locus_tag="VF226_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074995.1"
                     /translation="MDKELETVFLAGIKAMEDNRAAFRTRCKSKQPFCGRRSAFAANS
                     APE"
     gene            complement(750..941)
                     /locus_tag="VF226_13225"
     CDS             complement(750..941)
                     /locus_tag="VF226_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074996.1"
                     /translation="MIADGQCLRERRITIRQKEPVARLLDGFKDKLLTPELMKTSVSA
                     RRSDRTLPCPDQPGIGYFF"
     gene            complement(1008..1133)
                     /locus_tag="VF226_13230"
     CDS             complement(1008..1133)
                     /locus_tag="VF226_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074997.1"
                     /translation="MMEPYVGLDVSLEETAICVIDAAGRRRRHGGSAAFGRDWTG"
     gene            complement(1490..1798)
                     /locus_tag="VF226_13235"
     CDS             complement(1490..1798)
                     /locus_tag="VF226_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7074998.1"
                     /translation="MFRDVVFLLVNLFVVEPFQEELNGRLAQVGAPQAVVSEVAGCAS
                     VALPILVERVSSDPWWGTATIVRIWIGSTTPVVILGQTVPTCKTAIETARPYLGNAAA
                     "
     gene            2139..2411
                     /locus_tag="VF226_13240"
     CDS             2139..2411
                     /locus_tag="VF226_13240"
                     /inference="COORDINATES: protein motif:HMM:NF019016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 family protein"
                     /protein_id="HEX7074999.1"
                     /translation="MSLEFVRHDGIYNVDAEAVRFFATDGKASVMFTVSRKALSDLES
                     GENLSPRQIVAAYARHIAKVREVARKVYAAQGSGSPGAYILKTEHF"
     gene            complement(2899..3912)
                     /locus_tag="VF226_13245"
     CDS             complement(2899..3912)
                     /locus_tag="VF226_13245"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7075000.1"
                     /translation="MIRPETIRNRLLMLIAVLLLVAALHWSYPVTMPLVVTIFIIAAA
                     WPIKPWLDQMLPSSLSYACTVLALVLILTGFIAVIYVALAQVAQTFAQNQEQFRSLYE
                     TYATWARQKGLPVLGGEGGYGRLAAVAEVLFWRAYAVLGYLGLIAVLVIMGLPEVPAL
                     ERKFRDQLQAAENRELVDTVEQIAEKFRQYIGMTVLTSLITGATSAVWAFAIGLDLAL
                     IWGVLNFLLNFIPVIGNIIGIIPPTLYALIQFKGWTMPLVVFAGFVVLQITISNVIYP
                     MLQGRGMAMPPVTIIVALLFWGWVWGVAGALLAVPLTAALIIVCRHFKSTEGIAKILT
                     RDE"
     gene            3996..4193
                     /locus_tag="VF226_13250"
     CDS             3996..4193
                     /locus_tag="VF226_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075001.1"
                     /translation="MAPIGAFRLGDNRIAISASIGDYVSLHDVIAEARADIVDDVLTL
                     EQMVRAALVLEEQRDLGTAPG"
BASE COUNT          868 a         1313 c         1345 g          763 t
ORIGIN      
        1 tgtggctcgg ctttttccgg cggcgactgc atcggctgct gcggctcggc tttgggctta
       61 tcctgctccg gagcgtcggg ccgagtcggc gattgattct gagccatgtg agatcccttc
      121 gatgatgcga cagggatttg ccgcgactct cacatcgtac gacgcacctt catttacaat
      181 gaataaaatt cccgctttcg atctgaaaga ctcaaaattc cataaaaacg aacgttatca
      241 aggcggctct tttttatttc cgattctttt gacgaatctt gaagcggcgt cttcaatcgc
      301 aatcgatgaa ggaaactggc tcacatgaaa aagaatgggc agcctgctca agatagccgg
      361 agcttgctgc cggtcggtct ggtcaggcga gttacgaccc taataataag ccagcaccag
      421 tgctcctcct atcggtcagg gatgccgcag aattagctgg tagtcggatg agcagccgcc
      481 gctcagatgc gtggcacggg ctcgcgcttc cctatcgtgc tccctatcat aaaactttgg
      541 cgcgaacgcg gagggctcgt tcatggacaa ggaactcgag acggtgttcc tcgccggcat
      601 caaggccatg gaggacaatc gcgcggcttt caggacacga tgcaagagca aacagccgtt
      661 ttgcgggagg cgatcggcct ttgccgcgaa ctcggcgcca gaataaacga actgttcgaa
      721 cgtatcgccg tcctcgaggc acggctcgat cagaagaagt agccgatgcc cggctggtcc
      781 gggcatggca gagttcgatc gctgcgccgc gcggacacag acgtcttcat gagttcgggc
      841 gttaggagct tgtccttgaa gccatcgagc aagcgtgcca cgggctcctt ctggcgaatc
      901 gtgatgcggc gttcgcgcag acactgacca tcggcgatca cgatcgtgta aggcctacgg
      961 cagcagcgcc ttgcgcgacc tgatcgatgc cactggcgcc caagccgtca tcccgtccaa
     1021 tcgcgcccga aagccgctga tcccccatga cgccgccgcc taccggccgc gtcgatgacg
     1081 cagatcgccg tttcctccaa cgacacgtcg agccccacat acggttccat catcgctctc
     1141 ctgcatcacg gtggcgggaa gctggagtct cagtcttccc gctcgccgga gagccgtcac
     1201 ccagcattac accaggtgtg gaaaagctgg ccggcgtgac gaagatagtc ctcaacgaga
     1261 cgccggctcc ggagcccggc ggacatagca tgaggcggac gaggctgacg gagcgtcagc
     1321 cgcttgcccc ctcaatgcac cacatcaccc gcgctcatca gggcggcggt gttctgcgcc
     1381 gccccgatgg cagcaaggat ggcgccgcct ggcggccgca ccatctgggt cgcccgtgtc
     1441 ggatagcgcg gccgtccgtc gactcgcttt cacactctcg gcgttgcgct cacgccgcgg
     1501 cgtttcccag ataggggcga gccgtctcga ttgcagtttt gcaagtggga actgtctggc
     1561 caagtatgac aactggggtc gtgctgccaa tccagatgcg aacgatcgtg gcggtgcccc
     1621 accaaggatc tgaactcacc cgctcgacga ggatcggcag agcgaccgaa gcgcaaccgg
     1681 caacttcgga gacgacggcc tgcggtgctc caacctgtgc cagtctcccg ttgagttcct
     1741 cctggaaagg ctcgaccacg aacaggttga cgaggagaaa gacgacatcg cgaaacatac
     1801 tgacctcttg gccggcaaac gacgaagatg atccgacatc gcgcaagcgc cagcaggacc
     1861 tgcaccagag ggacccggat caggatgttc tgactaactg gccacttggg cgcatgaatc
     1921 aagccaagag tcggcgtctg ccgcacgacg aagtcagccg ccgcgtgacg ctgaaattgg
     1981 cgcactccgc gttgcggtgg gcattggcct gattgccgtt gaccgggggc ggcagggttg
     2041 ccagatcgtg agggctttca tcggcacact tgaatgctga ttgcgccccg cggctgggcg
     2101 gcgcatgatc cgccctcaca gccgaaaggg gtcaagcaat gtcgttggaa ttcgtccgcc
     2161 atgatgggat ctacaatgtg gacgcggagg cggtccgctt cttcgcgacc gacggcaagg
     2221 cttccgtgat gttcactgtt tctcgtaagg cgctgtccga cctggagagc ggagaaaatc
     2281 tcagcccgag gcagattgtg gctgcctatg cgaggcatat cgcgaaggtg cgtgaggttg
     2341 cacgaaaggt ctacgccgcc cagggttcgg gcagcccagg agcctacatt ctgaaaaccg
     2401 agcacttcta atgccgacag caagcggggg ccgggtcacg ccgcctcctt ccgatcgatg
     2461 ttgaccagat gcggccggaa gctcaccgca atcacgtctg gattcgcctc ccaggcgccg
     2521 gcgccgtgaa tgtttagcca taggcagcgg aacgcttccc gcgcattcgg cgagcctcca
     2581 cattgcatgg gaccgtcggc gcgccggatc ccacagcagc catcgcgccg ctgcagccgc
     2641 atgtcggtga cccaccttcg gcgatttcgg taaagcggat atcgggctat cacggccacc
     2701 gaaaccatgt tcggtaagat atctggttgc gattctgaac agcgaggcgg tccgcgagcg
     2761 cgtcgaagca cgaacgctcc ttgagccggt atgcaagcac atcattgagg atgcgggcgg
     2821 aacctacgag cctgcagcca tctgtgcccg acaaaccgta cgatggccgt gagggcccgt
     2881 ctgccaagcg agacgacgct actcgtcgcg ggtgagaatc ttggcgatcc cttcggtgct
     2941 cttgaagtgc cggcagacga tgatcagagc cgctgtcagc ggcacggcca gaagcgcgcc
     3001 ggcgacgccc cagacccagc cccagaacag aagtgcgacg atgatcgtga ccggcggcat
     3061 cgccatgccg cgcccctgca gcatgggata gatgacgttg ctgatcgtga tctgcagcac
     3121 gacgaagccg gcgaacacga cgagcggcat cgtccagccc ttgaactgga tcagggcgta
     3181 gagggtcggc ggaataatgc cgatgatatt gccgatgacc gggatgaaat tcagaaggaa
     3241 gttgagcacg ccccagatca gcgccagatc gaggccgatg gcgaaggccc agacggccga
     3301 cgtggcaccg gtgatcaggc tggtgaggac cgtcatcccg atgtattggc ggaacttctc
     3361 ggcgatctgc tcgaccgtgt cgaccaattc tcgattctcg gccgcctgca gttggtcccg
     3421 gaacttgcgc tcgagcgccg gcacctccgg caggcccatg atcaccagga cggcgatgag
     3481 gccgagatag cccagcacgg cgtaggcgcg ccaaaacaga acctcggcga cggcggcgag
     3541 gcggccatag cctccctcgc cgccgagaac gggcaggccc ttttgccgcg cccaggtcgc
     3601 gtaggtctcg tagagagagc ggaactgctc ctggttctgc gcgaatgtct gcgcgacctg
     3661 cgcgagcgcg acgtagatga ccgcaatgaa gccggtgagg atcaggacca aggcgaggac
     3721 ggtgcatgcg tagctgaggc tcgacggcag catctggtcc agccagggct tgatcggcca
     3781 ggcggcggcg atgatgaaga tcgtgaccac gagcggcatg gtcaccggat agctccagtg
     3841 cagcgccgcg acgaggagga gaacggcgat cagcatcagc agccggttgc gaatcgtttc
     3901 gggccggatc atcggaacct cgtcttcctc gtgggatgca atggcgagaa agtgttctga
     3961 acgagcaaga tcaatcgccg cgccaaagtt ccagcatggc gccgatcggg gccttccgac
     4021 ttggagacaa ccgtatcgcc atctccgcgt cgattggaga ctacgtctcc ctccacgacg
     4081 tcatcgccga ggccagggca gacatcgtgg acgacgtgct gactctggaa cagatggtcc
     4141 gagccgccct cgttctggaa gagcagcggg atctcggcac cgctccggga tgagcccgcc
     4201 ggagccggct cggttccgcc gaggagattc agtccggcgg gcgccccgga gcgcccggaa
     4261 acgctggcct ggcgcacgat cctgcttta
//
LOCUS       DASVDU010000207        14383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_401883, whole genome shotgun sequence.
ACCESSION   DASVDU010000207 DASVDU010000000
VERSION     DASVDU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14383
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401883"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VF226_13255"
     CDS             complement(<1..715)
                     /locus_tag="VF226_13255"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7075002.1"
                     /translation="MTDQTADLQLAADFEPATYEAWLALVAKVLKGSDFEARLVSSTL
                     DGIKIAPLYTRSDALPGAECAKPGEAPFTRGSKVAGEAAGWDIRQLHLATDPKEANAA
                     ILEDLRGGATSIALQVPAGGLGAALDGVQLDACPVALMARESTPEVAAELAALWKDRG
                     IADDARHGHFNADPLGTLAQSGQLTQPLDQNIERAVSLAVKAERWPGVTALLADGNPY
                     NAAGASEAQELAAMLSTLVA"
     gene            998..2509
                     /locus_tag="VF226_13260"
     CDS             998..2509
                     /locus_tag="VF226_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7075003.1"
                     /translation="MQQDGKIFIGKSSKPEYLDLKLANRHGLITGATGTGKTVTLQVL
                     AEGFSDAGVPVFAADIKGDLSGIAAKGEPKDFLDKRAADVGLTDYEQKAFPVVFWDLN
                     AKDNPPGVLEGHPIRATVSEMGPLLLARLLELNEVQEGVLNIAFRLAADEAMHLDDLS
                     DLRAVINSVGQRAAELTTKYGNVSAASVGAIQRRLLVLEEQGANEFFGMPALDIRDFM
                     RTTPDGRGNINVLAAEKLGKSPRVYAVFLLWLMTQLFDKLPEVGDPDKPKLVFFFDEA
                     HLLFDEAPKALLEQIERVTRLIRSKGVGIYYVTQNPLDVPDTVSAQLGNRVQHALRAF
                     SPREQKAVKAAASTFRPNPDLDTERVIMELKVGEALVSTLQDKGEPSIVQRTLIRPPS
                     SRIGPLTTEERTRLVENSPLFGKYENRQDRESASEILAKKAEQEAAEKARLEQEKQAQ
                     ESSWTKVIFGRGPRGGMSVGEQVVRDASRSVMRSMVTQVKNAILKSIFGSRRR"
     gene            2512..2853
                     /locus_tag="VF226_13265"
     CDS             2512..2853
                     /locus_tag="VF226_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7075004.1"
                     /translation="MMRSIGLALAMAVCVSAAPGAASAQQSLVERGRQIAEANCSRCH
                     AIGREGESRLPIAPPLRNLPSRYPVEELAEAFAEGIVTAHPEMPQFTFEPQEIEALLA
                     YIDSLGAAKAK"
     gene            complement(2860..4065)
                     /locus_tag="VF226_13270"
     CDS             complement(2860..4065)
                     /locus_tag="VF226_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7075005.1"
                     /translation="MQRAFVFLLAVTLSLLAAGCATLDTRTPVPNAMLASRAAPLDLQ
                     GLRAWGDEAPQDMTAEMRHRMPHLKRLAQSMGGRQPVVDILALSGGGTDGAFGAGLLA
                     GWTKRGDRPRFEIVTGVSAGAITAPFAFLGPRYDDKLREIWTRYGTSELIVAQLVPGL
                     LGGVALADSSPLQQLIARYIDRRVMSEIAAEYRKGRLLLVLTTNLDAQRPVIWNMGEI
                     AASPHPHALDLFRKVILASASIPGALPPVQIPVTVDGKEYQEMHVDGGTTREVFITPV
                     EAPLEAFDRFYASKPKRRIYIVKNGKYHPEYSPVRQQALPIAARAIETLIKTQHRNDI
                     YRIYRMAKDGNADFNLAFIPQSFQAKSNEVFDPRYQAALFEEGFNLALSGRAWVKAPP
                     DVAPNPKAK"
     gene            complement(4253..4852)
                     /locus_tag="VF226_13275"
     CDS             complement(4253..4852)
                     /locus_tag="VF226_13275"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7075006.1"
                     /translation="MTFKLPDLPYAYDALQPYMSAETLQFHHDKHHQAYVDTGNKLLQ
                     GSGLENKSLEEIVKAAYANKNQGLINNAGQHYNHTHFWPWMKKGGGGNKLPGKLEKLV
                     QDYGGFDKMRSDFINAGVTQFGSGWAWLTVNNGKLEVTKTPNGENPLMYGSQPILGCD
                     VWEHSYYIDYRNARAKYLEAWFDNLVNWEYVEGMLDKAS"
     gene            5310..6791
                     /locus_tag="VF226_13280"
     CDS             5310..6791
                     /locus_tag="VF226_13280"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7075007.1"
                     /translation="MTSRFRKYLFTLAVPVAALGAASLWLPNMIPGGNEKVTYETVEV
                     TRGPIRKVVSTSGPVRALVTVQVGSQLSGQIRSVSADYNTEVKEGDVLAELDAKTYKA
                     RVDQAKADLAAAKAQLANQEAAVIKAEANLRQAERVIERQQKLAERGISAQAALDTAI
                     RDAEVARADLIIAQAQVENAKAVIAQREAQLVQAQIDLDRTVIRSPINGTVISRTVDV
                     GQTVAASLQAPELFQIAQDLRRIRIEAQVNEADVGVIAEGNPATFTVDAYPDRTFHGR
                     VTQVRLAATELENVVTYTVIVEASNEDRRLFPGMTANLQIVTAERGDALRLSNDALRF
                     RPRDQSASSAANISPEQRAARIAERMKVEINLTDEQAAALSEELARRMAEASANQPTP
                     APAFGRGRRGGGNQGNAGQFMKRVEQIVAPLLTEDQRPLFEKWKRERENVRTATVWAL
                     GKNGTLERRFVRLGLGDDQFTEILGGNVKVGDLLVSRAREVRS"
     gene            6788..7540
                     /locus_tag="VF226_13285"
     CDS             6788..7540
                     /locus_tag="VF226_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075008.1"
                     /translation="MSEVATVARTAGSASPALIAAKGVSKIYTMGDQTVRALDDVSLE
                     IGEGEFVAIMGPSGSGKSSMMNLIGALDVPTSGSLSIDGRDISKMSSDELAHLRNRTI
                     GFVFQQFNLLPRTPALRQVMLPLLYASPKPGNAEELARKRLEQVGLASRMDHHPRQLS
                     GGQQQRVAIARALVNNPKILLADEPTGALDTKTTAEIMQLFCDLNEEGITVIVVTHEP
                     EVAEYAHRTIVFRDGRIVEDRQQSGYQRRAAE"
     gene            7537..8769
                     /locus_tag="VF226_13290"
     CDS             7537..8769
                     /locus_tag="VF226_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075009.1"
                     /translation="MSLFDSLFVALSALRTNLLRSVLTTLGIIIGVGSVIIMVAVGSG
                     ATSEIDKQIASLGSDMLVVYPGASRVRGRSAGAGTTLPLSERDLQAIRDKVPDVVAIS
                     GYLQGSAPVVYGNMNWTTSIGGIHDQYLTVRDWPLAEGRELTPQDVMSGARVAIIGQT
                     VAEKLFGGGDPIGAGIRIKNVPFEVIGVLSSKGQSSFGRDQDDIIFLPITAARGRIVG
                     RSQVVNDQVGQIYVKFDPRADLKVAQEDVEIVLRERRRIRPGAEDDFNVRNLAEFLKA
                     RTAALSTMSWLLGATSAISLIVGGIGIMNIMLVSVTERTREIGLRMAVGGRRRDILTQ
                     FLVEAVTLSLLGGIIGVAIGIGGAAIVAFSAEWPILINAQAVMVAMAAAGMTGILFGF
                     FPARRAAMLNPIDALRSE"
     gene            8867..9208
                     /locus_tag="VF226_13295"
     CDS             8867..9208
                     /locus_tag="VF226_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEX7075010.1"
                     /translation="MRLSTAFFAPRKSVVLRRALIPLAALSSLLLAGCATQSDYYGGA
                     QVAMEDDGRPAQIPPLLRAKPVEDDPTEPFSPNYGPPPPNMQDTAEKPEIPDDLPPAF
                     RRKLMNSISIS"
     gene            9366..9722
                     /locus_tag="VF226_13300"
     CDS             9366..9722
                     /locus_tag="VF226_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075011.1"
                     /translation="MNKSLVSLAAAAAFLIVATPGPKVQAAAATTVPMIKADASEIIQ
                     VRKRGKRFHRFHRHRRAHRRFYRHRHHHHHHRRWRGPRFSVFIGPPRCGWLRRRAIIT
                     GSHYWWRRYRRCLAWW"
     gene            complement(9772..10917)
                     /locus_tag="VF226_13305"
     CDS             complement(9772..10917)
                     /locus_tag="VF226_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX7075012.1"
                     /translation="MSAADNEPKIYRGLKDVYFERSATTFIDGKAGELRYRGYSIHDL
                     AEHSTFEETSYLLLYGELPNRTELARFDAELKSARQLPPPIFDIIAAVKSAHPMDVLR
                     TAVSALAAFDPETADNSREATLRKGVRLTSQVPMIVAAHEHIRNGREPVAADPSLSHA
                     ANFLWMLKGEKPSQDAARLLDTDFVLHAEHGSNASSFTARVVIGTDANIHAAVTAAIA
                     ALSGPAHGGAAEDVMRMAQEIGDPANVPAYVKAKRAAKEPITGFGHRVYRAEDPRARH
                     MREGVKRLSKEMGQPKWFEILEALVKEMEPYARHGINVNVDFYSGAIYHLHGIPNDLF
                     VPIFAIGRVPGWTVQILEQQANNILIRPLTLYSGPGPRAYVPIDQRG"
     gene            11007..11993
                     /gene="pta"
                     /locus_tag="VF226_13310"
     CDS             11007..11993
                     /gene="pta"
                     /locus_tag="VF226_13310"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX7075013.1"
                     /translation="MDIIADCVSAIRGRGLKVVLPEGDDERILRAARKLLDEGLAGPI
                     LLGNRAAIEGKAREIAATLTDIEIRDPEMDRRLEALAGVVASGREKMKPAMAQRLVRK
                     PVYFGGALVACGEADAMVAGAANPTRRVIEAGLMTVGLAPGVATPSSFFLMLLPAAHD
                     GAPRALVFADCAVNADPDAAMLADIAIASAASAEALLRQEARVAMLSFSTHGSATHPH
                     VEKVRSAVSLVRERMPGLKIDGELQADAALVEAVAAKKVKGVSEVAGRANVLVFPDLD
                     AGNIGYKLVQYLAGAKAIGPFLQGFAKPVSDLSRGASVDDIVSTAVVTLARA"
     gene            complement(12203..12421)
                     /locus_tag="VF226_13315"
     CDS             complement(12203..12421)
                     /locus_tag="VF226_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075014.1"
                     /translation="MSTKTMDTALDYLPAERKTRGPHGFLDSIRAFFTAIGEGIEAAH
                     RYERLTHQGTPPQKAVEIVFRDHFADRR"
     gene            12586..13164
                     /locus_tag="VF226_13320"
     CDS             12586..13164
                     /locus_tag="VF226_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7075015.1"
                     /translation="MTAKDDERRSGDGAFWISRLKDVGDASEATRAIDAIFFESSSVQ
                     HFESDAARRLFRERWLGRYLSRHPDEVFIAIANRDDVIGYLVGCLEDPLRDPLFADVA
                     YFGAFAPLLSSYPAHLHINVTAAWRSRGVGAQLIEAFASHAAASGTPGMHVVTAEGVR
                     NNGFYLARGFRQLATAEWSGKRVAFFGRKLGP"
     gene            13253..>14383
                     /locus_tag="VF226_13325"
     CDS             13253..>14383
                     /locus_tag="VF226_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX7075016.1"
                     /translation="MSEAAAEYDYIIVGAGSAGCVLANRLSADPNVRVLLLEAGGKDN
                     YIWVHIPVGYLYCFGNPRVDWGFETASEPGLNGRKLQYPRGKVLGGCSAINGMIYMRG
                     QARDYDHWRQLGNSGWGWEDVLPYFLKHEDQWALEPEEFGGAHARGGEWRVEQPRVRW
                     EILDAWAEAAEQAGIPRVRDFNGGNNEGTSYFHVNQRKGWRWSSAKGFLRPALNRSNL
                     TLLTHATVERLTLEGKRVTGLTYRQNGAGRRTAAARREVILAAGAIGSPMILQLSGIG
                     PGDVLKSAGIDVKHELKGVGENLQDHLQIRVAFKVSGVRTLNERSQKLWQKAGIALEY
                     ALKRSGPMSMAPSQLGCFTKSDPSRDTPNLQYHIQPLTLPKFG"
BASE COUNT         2566 a         4451 c         4744 g         2622 t
ORIGIN      
        1 aggccaccag cgtcgaaagc atcgcggcga gctcttgggc ctcgctcgcg ccggcggcgt
       61 tgtacggatt gccgtccgcg agcagggcgg tgacgccggg ccagcgctcg gccttgacgg
      121 caagggagac cgcccgttcg atgttctggt cgagcggttg agtgagttga cctgactggg
      181 cgagcgtgcc tagcggatcg gcattgaaat ggccgtgccg cgcgtcgtcc gcgatgccgc
      241 ggtctttcca gagggccgcc agctctgccg ccacctctgg cgtgctctcg cgcgccatga
      301 gcgcgactgg gcaggcatcg agctggacgc catcgagggc ggcgccaaga ccacccgctg
      361 ggacctgcaa cgcgatcgag gtggcgccac cgcgcagatc ttccagaatg gctgcgttcg
      421 cctccttggg gtcagtggcg agatggagct gacgaatgtc ccagccggca gcctctcccg
      481 ccaccttgct gccgcgcgtg aagggagctt cgccgggctt ggcgcactcc gcgccgggca
      541 aggcatccga ccgcgtataa agcggagcga tcttgatccc gtcgagcgtg gacgagacga
      601 gccgggcctc gaagtcgctg cccttcaaga ccttggcgac gagggccagc caggcctcat
      661 aggtcgccgg ttcgaaatcg gcggcaagct gcagatcagc ggtttgatcg gtcatcggta
      721 aagaatcccg cgtgcggcag ggctgggccg gggaggtgcg ccggtcagct cagccaaatt
      781 tcgcacgcac attactcgat cttggctgag acgccatgcc cttggccagg aaacgcaacg
      841 agcgatgctt cgggcctcct tccagcgttt tccccggatg agatgcgagg atgctttgca
      901 ctcgggcgat ggcgtgccaa gacggatact gaggatgaag agcacgaatc gcggcggttc
      961 gaggtcggtg ccgcggtgag aattggggag ccagtcgatg cagcaggacg gcaagatctt
     1021 cattggcaaa agcagcaagc ccgagtacct cgatctcaag ctcgccaatc gccacgggct
     1081 gatcaccggc gcgacgggca ctggcaagac cgtgacgctg caggtgctgg cggaaggctt
     1141 ctcggacgcg ggcgtgccgg tgtttgccgc cgacatcaag ggcgacctct ccggcatcgc
     1201 cgccaagggc gagccgaagg acttcctcga caagcgcgcg gccgacgtcg ggctgaccga
     1261 ttacgagcag aaggcctttc cggtcgtctt ctgggacctc aacgccaagg acaacccgcc
     1321 cggcgtcctc gaaggccatc cgatccgggc gacggtctcc gagatggggc cgttgctgct
     1381 cgcgcgcctc ttggaattga acgaggtaca ggagggcgtc ctcaacatcg ccttccgcct
     1441 ggccgccgac gaggccatgc acctcgatga tctttctgac ctccgtgcgg tcataaatag
     1501 tgtcggacaa cgggcggccg agctcaccac caaatacggc aacgtctccg cggcatcggt
     1561 cggggcgatc cagcggcgtc tcctggtgct ggaggaacag ggggccaatg agttcttcgg
     1621 gatgcccgcc ctcgacatca gggacttcat gcgaacaacg ccggatggcc ggggcaacat
     1681 caatgtcctc gccgccgaga agctggggaa atcgcctcgc gtttacgccg tcttcctgct
     1741 ctggctgatg acgcagcttt tcgacaagct gcccgaggtg ggcgatccgg acaaaccaaa
     1801 gctcgtcttc ttcttcgacg aggcgcatct cctgtttgat gaggccccta aggcgctctt
     1861 ggagcagatc gagcgcgtga cccggcttat ccgatccaaa ggtgtcggca tctactacgt
     1921 tacacagaac ccgctcgacg tgccggatac ggtgtccgcg cagcttggca atcgcgtgca
     1981 gcatgcgctt cgggccttca gcccgcgcga gcagaaggcg gtcaaggctg cggcatccac
     2041 gttccgcccg aatcccgatc tcgacaccga acgcgttatc atggagctca aggtcggcga
     2101 ggcgctggtc tcgacgctgc aggacaaggg cgagccgtcg atcgtgcagc gaacgctcat
     2161 ccgtccacct tcgagccgca tcggccctct gacgacggag gagcggacac ggctcgtcga
     2221 gaacagcccg ctgttcggca agtacgaaaa tcggcaggat cgcgagtcgg cgagcgagat
     2281 cctcgccaag aaggcagagc aggaggctgc ggaaaaagcg cggctcgagc aggaaaagca
     2341 ggctcaggaa agcagctgga cgaaggtcat cttcggccgt ggtccgcgtg gtggcatgtc
     2401 cgtgggcgag caggtcgtgc gcgatgcctc gcgctccgtc atgcgctcga tggtgacgca
     2461 ggtcaagaac gccattctca agtcgatctt cggcagccgg agacgatgat catgatgcga
     2521 tcgatcgggc tggctctggc aatggcggtt tgcgtgtcgg ctgcgcctgg agcggcctca
     2581 gcgcaacagt cgctggtgga acgcggccga cagatcgccg aggcgaactg ctcccgctgc
     2641 catgccatcg gacgcgaggg agagagccgc ctgccgattg ctccaccgct tcgcaacctg
     2701 ccgagccgct atccggtcga ggaactcgcg gaggccttcg cggagggcat cgtgacggcc
     2761 catccggaaa tgccccagtt caccttcgag ccgcaggaga tcgaggctct gttggcctat
     2821 atcgacagcc tcggggcggc gaaggcgaaa tgagatcgct cactttgcct tcggattcgg
     2881 ggcgacatcc ggcggcgcct tcacccaggc gcgtccggac agcgcgaggt tgaagccttc
     2941 ctcgaaaaga gccgcctggt agcgtggatc gaagacttcg ttggacttcg cctggaagct
     3001 ctgcgggatg aacgcaaggt tgaagtccgc atttccgtcc ttcgccatgc gatagatccg
     3061 gtagatgtcg ttccggtgct gcgtcttgat cagcgtttcg atggcgcggg ctgcgatggg
     3121 gagcgcctgc tggcgcacgg ggctgtactc cgggtgatac ttcccgttct tgacgatgta
     3181 gatccgccgc ttcggtttgg acgcgtagaa gcggtcgaag gcttcgagcg gtgcttcgac
     3241 aggtgtgatg aaaacctcgc gcgtcgtgcc gccgtcgacg tgcatctcct ggtactcctt
     3301 gccgtcgacg gtcactggga tctggaccgg tggcaaggcg ccgggaatcg aggccgaggc
     3361 gaggatcacc ttgcggaaca agtcgagcgc gtgcggatgc gggcttgcag cgatctcgcc
     3421 catgttccag atgacgggcc gctgtgcgtc gagattggtg gtgagcacga gcagaagacg
     3481 gcccttccgg tactccgccg cgatctctga cataacgcgc cggtcgatgt agcgcgctat
     3541 cagctgctgc agcgggctgc tgtcagctag ggcaacgccg ccgaggagac ccggcacgag
     3601 ctgggcgacg atcagctcgc tcgtcccata gcgggtccag atctcccgca gcttatcgtc
     3661 atagcgaggc ccgaggaaag cgaacggagc cgtaatggcg ccggcgctga ccccggtgac
     3721 gatctcgaaa cggggccggt cgccgcgttt cgtccagccc gcaaggagac ccgcgccgaa
     3781 tgcgccatcg gtgccgccgc ctgaaagagc gaggatgtcg acgacgggct gccgtccgcc
     3841 catcgactgg gccagccgtt tcagatgggg catgcggtgc cgcatctcgg cggtcatgtc
     3901 ctgaggcgct tcgtcgcccc aggcgcgcaa tccttggagg tccaacggcg ctgcgcgcga
     3961 tgccagcatc gcattcggca caggtgtgcg cgtgtcgagc gtcgcgcaac cggccgccag
     4021 caatgacagc gtcaccgcca gcaagaatac aaaagcgcgc tgcaccccat tcccccggag
     4081 tcgatgtggt ccgccatcct acgcggcaag gacgccgtct gtcagccgga tgcgtatgcg
     4141 ccgatgttgg tgctcaaaat gagacgaacc gatgaattcg gacacaaaag aaggggccgc
     4201 gccaaccaac acggcccctt gtcagtcaat cgcctagcgc gatcggttta gatcaggacg
     4261 ccttatcgag catgccctcg acgtattccc agttcacgag attgtcgaac caggcctcga
     4321 ggtatttcgc ccgcgcgttg cggtagtcga tgtaatagct gtgctcccac acgtcacagc
     4381 cgaggatcgg ttggctgcca tacatgagag ggttctcgcc gttcggcgtc ttcgtcacct
     4441 cgagcttgcc gttgttgacg gtgagccagg cccagccgga gccgaactgc gtgacgccgg
     4501 cgttgatgaa gtcggaccgc atcttgtcga agccgccgta gtcctggacc agcttctcga
     4561 gcttgccggg cagcttgttg ccgccgccgc ccttcttcat ccacggccag aagtgcgtgt
     4621 gattgtagtg ctggccggcg ttgttgatca ggccctggtt cttgttcgca taggcggcct
     4681 tgacgatctc ctccaggctc ttgttctcga ggcccgagcc ctgaagcagc ttgttcccgg
     4741 tgtcgacgta ggcctggtga tgcttgtcat gatgaaactg cagcgtctcg gctgacatat
     4801 acggctgcag cgcatcgtaa gcgtaaggca ggtcaggaag tttgaaggtc acggcagtcc
     4861 tccgtcgcgt tgaattagac ctaggaatgc gggggtgggg ctgcaaacta gcccttcact
     4921 ccctgcaaat caaacgccgt cggaggggtg agggtgccaa aggtggcgtt aagagaagaa
     4981 ggggcgcggt cgaccctgag cgccgcgccc catggatcac gccggggaag tcatttcagg
     5041 gtagtctcac cctagtggag gccggttgaa cacactctga atgctgtatt catctgtcgt
     5101 tcatgtgcga atgaccgcat tcgcccgtgt ccaggcaagg cctctcaact atccagccgc
     5161 cgtttttcct gccaagttcg caataatttt caggcgtggc catcggcgcc aggtatacgc
     5221 tttcgcctca gcttccattc atccgacggg ttccccgctc ctatgacgtt catcgttcgg
     5281 ttcgtccgct tcagacaggc ataacacaga tgacttcgcg cttcagaaaa tacctgttca
     5341 cgctggctgt tcccgtggct gcgcttggcg ctgcaagcct ctggttgccg aacatgatcc
     5401 ctgggggaaa cgagaaggtc acctatgaga cggtggaggt cacgcgtggc ccgatccgga
     5461 aggtggtctc gacgtcgggg cccgtgcgcg cgctcgtgac cgtgcaggtg ggctcgcagc
     5521 tttccggcca gatccggagc gtctcggcgg actacaacac cgaggtcaag gaaggcgacg
     5581 tcctcgccga gctcgacgcc aagacataca aggcgcgtgt cgatcaggcg aaagcggatc
     5641 tcgcggcagc gaaggcacag cttgccaatc aggaagcggc cgtcatcaag gccgaagcca
     5701 atctgcgtca ggcagagcgg gtcatcgagc gccagcagaa gcttgcagaa cgcgggattt
     5761 cggcgcaggc cgcgctcgat acggccatcc gcgatgccga ggtggcccgt gctgatctca
     5821 tcatcgcgca ggcgcaggtc gagaatgcca aggcagtcat cgcccagcgc gaggctcagc
     5881 tcgtccaggc gcagatcgat ctcgaccgta cggtgatccg ttccccgatc aacggcaccg
     5941 tcatttcccg cacggtcgac gtcggtcaga cggtggcggc aagcctgcag gcgccggagc
     6001 ttttccagat cgcgcaggat ctccgccgta ttcgaattga agcgcaggtc aacgaggccg
     6061 atgtcggcgt cattgccgag ggcaacccgg cgaccttcac cgtcgatgcc tacccggatc
     6121 gcaccttcca tggccgcgtg acccaggtgc ggcttgccgc gacggagctc gagaacgtcg
     6181 tcacctatac ggtgatcgtc gaggcctcga acgaggatcg gcgtctgttt ccggggatga
     6241 cggccaacct gcagatcgtg acggccgaac gcggcgacgc cttgcgcctg tccaatgacg
     6301 cccttcggtt caggccgcgc gatcaaagcg cgagcagcgc ggcgaatatt tcgcccgagc
     6361 agcgcgccgc gcgaatcgcc gagcgcatga aggtcgagat caacctgacc gacgagcagg
     6421 ccgcggcgct cagcgaggag ctggcccgca ggatggctga ggccagtgcc aaccagccga
     6481 cgccggcgcc tgccttcggc cgggggcgcc gtggcggtgg caatcagggc aacgccggcc
     6541 aattcatgaa gcgtgtcgag cagatcgtgg cgccgctgct caccgaagat cagcgcccgc
     6601 tgttcgaaaa gtggaagcgg gagcgcgaga acgtgcgcac cgcgacggtc tgggcgctcg
     6661 gcaagaacgg gacgctggag cggcggttcg tgaggctcgg cctgggagat gaccagttca
     6721 cggaaatcct cggcggcaac gtgaaagtgg gcgacctgct ggtgagccgc gcgcgggagg
     6781 tgcggtcatg agcgaggtcg caacagtggc ccgcaccgcc ggttccgcga gccctgcgct
     6841 gattgcggcc aagggcgtgt ccaagatcta cacgatgggc gatcagacgg tgcgcgcgct
     6901 cgatgatgtg tcgctcgaga tcggcgaggg cgagttcgtg gccatcatgg gcccctccgg
     6961 ctccggcaaa tcgagcatga tgaacctcat cggcgcgctc gacgtgccga cctcgggcag
     7021 tctttcgatc gacggccgcg acatctcgaa gatgtcctcc gacgagctgg cgcatttgcg
     7081 caaccgcacc atcggcttcg ttttccagca gttcaacctg ctgccgcgca cgcccgcgct
     7141 gcgccaggtc atgctgccgc tgctctatgc cagcccgaag ccgggcaacg cggaggagct
     7201 ggcgcggaaa cggctggagc aggtcggcct cgcaagccgg atggaccatc acccgcggca
     7261 gctctccggt ggtcagcagc agcgcgtcgc catcgcgcgc gcgctcgtca acaatcccaa
     7321 gatcctgctg gccgacgagc cgaccggcgc gctcgataca aagaccactg cggagatcat
     7381 gcagctcttc tgcgatctca acgaggaagg catcacggtg atcgtcgtca cgcacgagcc
     7441 ggaggtggcc gagtatgccc accggacgat cgtgttccgc gacgggcgca tcgtcgagga
     7501 caggcagcag tccgggtacc agaggagggc cgccgaatga gcttgttcga cagcctgttc
     7561 gtcgcgcttt cggcgcttcg gaccaatctg ttgcgatcgg tgctgacgac gctcggcatc
     7621 atcatcggcg tcggttcggt catcatcatg gttgccgtcg gttcgggcgc cacgagcgag
     7681 atcgacaagc agatcgcctc gctcggctcc gatatgctcg tcgtctatcc gggcgcctca
     7741 cgggtgagag ggcgctcggc cggcgcgggc acgacgctgc cgctctccga gcgtgacctg
     7801 caagccattc gcgacaaggt gcctgatgtc gtggccatct ccggttacct gcaaggcagc
     7861 gcgcccgttg tctacggcaa catgaactgg acgacctcga tcggcggcat ccatgaccaa
     7921 tatctgaccg tccgggactg gccgctcgcc gaaggccgtg agctgacgcc ccaggacgtg
     7981 atgtccggtg cgcgcgtggc gatcatcggc cagacggtcg ccgagaagct gttcggcggc
     8041 ggggatccga tcggcgcagg catccgcatc aagaacgtcc cgttcgaggt catcggcgtg
     8101 ctgtcgtcga agggccagtc gagcttcggc cgcgatcagg acgacatcat tttcctgccc
     8161 atcaccgcgg cgcgtggccg gatcgtcggc aggagccagg tcgtcaacga tcaggtcggt
     8221 cagatttacg tgaagttcga tccgcgtgcc gatctgaaag tggcgcagga ggacgtcgag
     8281 atcgtgctgc gcgaacggcg gcgcatcagg ccgggcgcgg aggacgattt caacgtgcgc
     8341 aacctcgcgg agtttctgaa ggcccgcacg gccgcgcttt cgaccatgag ctggcttctg
     8401 ggcgcgacgt ccgccatttc gctcatcgtc ggcggcatcg gcatcatgaa catcatgctg
     8461 gtgtcggtaa cggagcgcac gcgcgagatc ggcctccgga tggctgtcgg cggccgccgc
     8521 cgcgatattc tcacgcaatt tctcgtggag gccgtcacgc tcagcctgct gggcggcatc
     8581 atcggcgtcg ccatcgggat cggcggcgcg gccatcgtcg ccttctctgc cgaatggccc
     8641 atcctcatca atgctcaggc ggtgatggtg gcgatggcgg cggcaggcat gacgggcatc
     8701 ctgttcggat ttttcccagc acggcgggcg gccatgctca atccgatcga cgcgctgcgg
     8761 agcgaatagt tgagtctgga ccatttctgc acgccggcta acgcgttctt aaccacggcc
     8821 gggttctgat ggtcggcagt ctcgcgtctg ttggagttcg tgtgcgatgc gtctgtcgac
     8881 cgcatttttc gcgccgcgca aaagcgtcgt attgcgccgc gccctgatac cgctcgcggc
     8941 attatccagt ctcctcctcg ccgggtgtgc cacccagtcc gattactacg gcggcgcgca
     9001 ggtcgccatg gaggatgatg gtcgtcccgc ccagattccg ccattacttc gggccaaacc
     9061 agtcgaagac gaccctacgg agccgttcag tccgaattac ggtccgccac cgccgaatat
     9121 gcaggacacg gcagaaaaac cggagattcc ggacgatttg ccaccggcat ttcgacgaaa
     9181 acttatgaat tcgatcagca tttcttaggt gctgtcggtc aaaaataggg atctgccggg
     9241 aactatgggt tggacaactc cgtttcccgt actacgatat ccgggatcac gtggggaagc
     9301 tcagcagatg gtggtttgcc gtaccctgtt gaaggggtga cggcccatta ttcgggagcg
     9361 tcgacatgaa caaaagccta gtttcactgg cggcagctgc tgccttcctc atcgtcgcga
     9421 caccagggcc aaaggtccag gccgcggcgg caacaaccgt tccgatgata aaggcggatg
     9481 cttcggaaat cattcaggtg cgcaagcggg gcaaacgctt ccatcgcttc caccgtcatc
     9541 gtcgcgccca ccgccgcttc tatcgccatc gtcatcacca tcatcaccat cggcgttggc
     9601 gcgggccgcg cttcagcgtg ttcatcggcc ctccgcgctg cgggtggttg cgccgtcgag
     9661 cgatcatcac cggcagccat tactggtggc ggcgttatcg gcgttgcctg gcatggtggt
     9721 gagcgggatc tgaaaacggg ccgaatggcc cgttttcttc attgtcaaag ctcagccgcg
     9781 ctgatcgatc ggaacatagg cgcgcggtcc ggggccgctg taaagcgtca gcggccggat
     9841 gaggatattg ttggcctgct gctccaaaat ctgcacggtc cagccgggga cgcggccgat
     9901 agcgaaaatc ggaacgaaaa gatcgttcgg aatgccgtgc aaatgataaa tggcgcctga
     9961 atagaaatcg acgttgacgt tgatgccgtg gcgggcatag ggctccattt cctttacgag
    10021 cgcctcgagg atttcgaacc acttcggctg gcccatttcc ttcgatagcc gcttgacgcc
    10081 ttcccgcatg tgacgcgctc gcgggtcctc ggcacggtag acacggtggc cgaagccggt
    10141 gatgggctcc ttggccgcgc gcttcgcctt gacgtaggcc ggcacattgg ccgggtcgcc
    10201 gatctcttgt gccatgcgca tcacgtcctc ggccgcgccg ccgtgggcgg ggcccgacag
    10261 ggccgcaatg gcggcggtca cagctgcgtg gatattggca tcggtcccga tcaccacccg
    10321 cgcggtgaag gacgaggcgt tggagccgtg ctcggcgtgc agcacgaaat cggtgtcgag
    10381 cagcctcgca gcatcttgcg acggcttctc gcccttcagc atccagagga agtttgccgc
    10441 gtgggacagg gacgggtccg ccgcaacggg ctcgcggcca ttgcggatgt gctcgtgggc
    10501 ggcaacgatc atcggcacct gcgaggtgag gcgcacgcct ttgcgtaagg tcgcctcgcg
    10561 gctgttgtcc gcggtctccg gatcaaaggc ggcgagggcg gagacagccg ttcggagcac
    10621 gtccatcggg tgcgcagact tcacggccgc gatgatgtcg aagatcgggg gcgggagctg
    10681 gcgtgccgac ttgagctccg cgtcgaacct ggcgagttcg gtcctgttcg gcagctctcc
    10741 atagagcagg agatagctcg tttcctcaaa cgtcgagtgc tcggcaaggt catgaatgga
    10801 gtaaccgcga tagcgcagct cgcccgcctt gccgtcgatg aacgtcgttg ccgagcgctc
    10861 gaaatagacg tccttcagtc cgcgatagat tttgggctcg ttgtcagcgg cgctcatggg
    10921 cttgatcctc cgttgcggaa ttgcgatgcc atggtctgat cttgcaggcc cggatggctt
    10981 gccacgaaca catgagggag gcctgaatgg acatcatcgc cgactgcgtg tccgcgatca
    11041 gaggccgtgg gctcaaggtg gtgctgcccg agggagatga cgaacggatc ctccgggcgg
    11101 cgcgcaaatt gctcgacgag gggcttgccg ggccgattct gctcggcaac cgcgcggcga
    11161 tcgagggtaa ggccagagag atagcggcca cgttgaccga catcgagatc cgcgatcccg
    11221 aaatggatcg gcgactcgag gcactcgcgg gcgtcgttgc ttccggccgc gagaagatga
    11281 agccggccat ggcgcagcgc ctggtgcgca agccggtgta tttcgggggc gcgctggtcg
    11341 cttgcggcga ggccgatgcc atggtcgccg gggcggcgaa ccccacgcgc cgggtgatcg
    11401 aggccggcct gatgacggtt gggctcgcgc ctggggttgc cacgccgtcg agctttttcc
    11461 tgatgctgct tccggctgcc cacgacggtg cgccacgcgc actcgtcttt gccgactgcg
    11521 cggtgaacgc cgaccccgat gctgccatgc tggccgacat cgcaatcgcg tcggccgcca
    11581 gcgccgaagc cttgctgcgg caggaggcgc gggtcgccat gctctcgttc tctacgcacg
    11641 gaagcgccac tcacccgcac gtcgagaagg tccgatcggc ggtttcgctc gtccgggagc
    11701 ggatgccggg gttgaagatc gacggcgagc ttcaggccga cgcggccctg gtggaggcgg
    11761 tcgctgccaa gaaggtgaag ggcgtgagcg aggttgcggg gcgcgccaac gtgctcgtct
    11821 tccccgatct ggacgccggc aacatcggct acaagctcgt ccagtatctg gccggcgcaa
    11881 aggccatcgg gccgttcctg caaggattcg cgaaacctgt ttccgacctg tcgcgcggtg
    11941 cgagcgtcga cgacatcgtc tctactgccg tcgtcacgtt ggcgcgtgcc taaccgcttg
    12001 cggcgctctg gggcgccacg gcgccggaat gtcggcgttg catcgcagcg gcgcatccga
    12061 tcttttccgg gacaattcgt ctcgtttatc agaccgcatc taggaacgac cgctagcgac
    12121 tgtcaatgcc aatcggccct ggataaaaca aagccgccgt gcaggcgctg cacggcggac
    12181 cagtcgtcct aacctgggga ggtcagcggc gatccgcgaa gtgatcgcgg aagacgatct
    12241 cgactgcctt ctggggaggc gttccctgat gcgtcagccg ctcatatctg tgggccgctt
    12301 cgataccctc gccgatggcg gtgaagaagg ctcgtatgga gtcgagaaag ccatgcgggc
    12361 cgcgggtctt ccgctcggcg ggaaggtagt cgagggccgt gtccattgtc ttggtgctca
    12421 tgatcaaacc tcgtgatccg gctcgggccg tgcgttatcg cttcgccctt gcacacttgg
    12481 tatatggcat accacattgc gccgcacaat gtgacaatct gtctaccagc tattcgtgat
    12541 ctgcatggat gacatcagca gtcggatgcc gcggggacat atcggatgac cgcaaaggat
    12601 gacgagcgac gatccggcga tggtgcattc tggatttctc gcctgaagga cgtgggtgat
    12661 gcgagcgaag ctacaagggc aatcgacgcc atcttcttcg aatcaagcag tgttcaacat
    12721 ttcgaaagcg acgcggcgcg acggcttttc cgtgagcgat ggctcggccg ctatctgtcg
    12781 cgtcatccgg acgaggtgtt catcgccatc gccaatcgag atgacgtgat cggatatctc
    12841 gtcggttgcc tcgaggatcc attgcgcgat cctctctttg ccgacgtcgc atacttcggc
    12901 gccttcgcgc cgctgttgtc ctcctatccg gcgcacctgc acatcaacgt caccgcagcc
    12961 tggcgcagcc gaggcgtggg cgcccagctc atcgaggcgt ttgcgagcca cgccgcagcg
    13021 tccggaacgc cgggaatgca tgtcgtgacg gcggaaggcg tccgcaacaa cggcttttac
    13081 ctcgcgcgcg gattccggca gctggcgacc gccgaatgga gcggcaaacg cgtcgccttc
    13141 ttcggccgta agctggggcc gtgagcgcgc cggagcggac cttccgtcgc cggcgccgct
    13201 cggttaagta ggtgcaaggc gaacagtgga aatcgagggg agtagagccg ccatgtccga
    13261 agccgctgca gaatacgact acatcatcgt cggcgccgga tcggcgggct gcgtgctggc
    13321 caacaggctg tcggccgatc cgaacgtacg cgttctgttg ctggaggccg gcggcaagga
    13381 caattacatc tgggtgcata ttcccgtcgg ttatctctac tgctttggca atccacgggt
    13441 cgactggggc ttcgagacgg cgagcgagcc ggggctcaac ggccgcaaac tgcagtatcc
    13501 gcgcggcaag gtcttaggcg gctgctccgc catcaacggc atgatctaca tgcgcggcca
    13561 ggcccgcgac tacgatcact ggcgccagct cggcaattcg gggtgggggt gggaagatgt
    13621 gctgccctac ttcctcaagc atgaggacca gtgggcgctg gagcccgagg agttcggcgg
    13681 cgcccacgcc cggggcggtg agtggcgggt cgagcagccg cgcgttcgtt gggagatact
    13741 cgatgcctgg gccgaagccg ccgagcaggc cggcattccg cgtgtgcgcg acttcaacgg
    13801 cggcaacaac gaaggcacgt cctacttcca tgtcaatcag cgcaagggct ggcgctggtc
    13861 ctcggccaaa ggctttttgc gtcctgcgct gaaccggtcg aatcttacgc tgctcacgca
    13921 tgccacggtc gaaaggctga cgctcgaggg caagcgggtg accggcctga cctatcgcca
    13981 gaacggagcg ggccgccgca cggctgcggc gcggcgcgag gtgatactgg cagcgggcgc
    14041 catcggctcg ccgatgatcc tgcaactgtc cggcatcggt ccgggggacg tgctcaagtc
    14101 cgccggtatc gatgtgaaac atgagctgaa aggcgtcggc gagaacctgc aagaccatct
    14161 gcagatccgc gttgcgttca aggtgtcggg cgtcaggacc ttgaacgagc gctcgcagaa
    14221 gctctggcag aaggcaggca ttgcgctcga gtacgcactg aagcggagcg gacccatgtc
    14281 gatggctccc tcccagctcg gctgcttcac caaatccgat ccgagccgcg acacgcccaa
    14341 tctgcagtat cacatccagc cgctcacgct gccgaagttc ggc
//
LOCUS       DASVDU010000208        11539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_13883, whole genome shotgun sequence.
ACCESSION   DASVDU010000208 DASVDU010000000
VERSION     DASVDU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13883"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..405)
                     /gene="soxZ"
                     /locus_tag="VF226_13330"
     CDS             complement(79..405)
                     /gene="soxZ"
                     /locus_tag="VF226_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX7075017.1"
                     /translation="MVKSTPRVRMPRKAKAGEVIEVKTLISHEMESGQRKDAQGKPIP
                     RKIIKMFTAKFNGKEIFHSDWHPAISANPYLAFSVRVPETGTFEFTWLDDDGTIFKHE
                     QKVTVA"
     gene            complement(437..865)
                     /gene="soxY"
                     /locus_tag="VF226_13335"
     CDS             complement(437..865)
                     /gene="soxY"
                     /locus_tag="VF226_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX7075018.1"
                     /translation="MLIAVGAAAAALVMAPRLTMAKPEQVEAELKKLYGDKPRTEGKI
                     KLELPSIAENGQVVPLKFEVESPMTENDYVKAVHIFADGNPNPGVASFYFTPLMPKAA
                     AQFRIRLAGTQNVVAVAEMADGTLHSAKQEVKVTIGGCGG"
     gene            1054..1893
                     /gene="soxA"
                     /locus_tag="VF226_13340"
     CDS             1054..1893
                     /gene="soxA"
                     /locus_tag="VF226_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX7075019.1"
                     /translation="MKPVRIRDFVPLLAPLLLMLAAAPGLAQETVDSEKAIEQYRQML
                     KEDPWSNPAMLDVDRGEQLWKTPRGPKSVSLEGCDLGKGPGVLEGAFAELPRYFSDAD
                     RVMDLEARIVWCMEKLQGFDRKELVTKPYPKGGSPTKDIGAIATFVAYQSNGLKISPK
                     LEHAKEKEALALGEALFFRRQGPMDFSCATCHADKGKRIRLQNLPYLSDPSEARKVVG
                     EWPAYRVSSSHVMTMQHRIYDCFWQMRLPEVELGSDVPVALTMYLAKMAEGGEISAPG
                     LKR"
     gene            1907..2524
                     /gene="soxX"
                     /locus_tag="VF226_13345"
     CDS             1907..2524
                     /gene="soxX"
                     /locus_tag="VF226_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEX7075020.1"
                     /translation="MIRNICAAAILVLSGSIPAFAQSPEVSGDRVQAAIKASWPNQPP
                     EWQSRLEQDETMRLCSQYRNDPPSDVAEAIMARERSNIKYPADGKLMGDWKKGEKLAQ
                     SGYGRRFTDYPPRRENGGNCYACHQLAKEEVSFGTLGPSLLEYGKIREFSEADVKAVY
                     ERIYNPQAFQACANMPRLGANGHLTIEQIKDLVAYVMSPDSPVNK"
     gene            2551..2937
                     /locus_tag="VF226_13350"
     CDS             2551..2937
                     /locus_tag="VF226_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7075021.1"
                     /translation="MRALAGLIISAIASVLVGGSALAAELVMFEDPACPYCRRWHAEV
                     GVSYHATEEGRIAPLRRVDIKDRYKAGVSLKGPVTFTPTFVLAEDGREVGRIVGYSGA
                     DFFYGFLANLLEQRASSPARTRGLSR"
     gene            3033..3413
                     /locus_tag="VF226_13355"
     CDS             3033..3413
                     /locus_tag="VF226_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7075022.1"
                     /translation="MSRILSNKVVEALQSDVELSPELTRIMKNARAASDFLKALAHEH
                     RLILLCLLAERERSVTELEELLSLRQTTVSQQLARLRFDGIVTTRRVGKVVYYSLADT
                     STLKFVKVIYDMFCSDPKPEKDSA"
     gene            3474..4532
                     /locus_tag="VF226_13360"
     CDS             3474..4532
                     /locus_tag="VF226_13360"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX7075023.1"
                     /translation="MDGLIAPVGGFICGGLAGFAAQYGRLCTIGAMEDALVAGDFRRA
                     GAWALSAAVAVLLTQGLVFAGALELSGIHYVRPMLDITGLVIGGLLFGFGAALVGTCA
                     FGLLVRLGTGDLRALVCALVVGLAAFSATGGILSPLRLALSEFGALDTSAIGGKTLTG
                     IIGASPHSAASFLAVLLVAGALLVFALTNWRLYRKPRLIAASCLMGLAVAGGWLTTGV
                     LADPFSGQRPESLTFVIPLGRLLLQLVGERLSSTEFAVATVFGVSAGSFAVAAVRDEL
                     RWEAFDDHREMRRHLLGALLMGFGGVLARGCTIGQGLTGMSVLSLAAPVAVFAMVVGA
                     RAGLVYLVEGRRLFGQRY"
     gene            complement(4710..5816)
                     /gene="dctP"
                     /locus_tag="VF226_13365"
     CDS             complement(4710..5816)
                     /gene="dctP"
                     /locus_tag="VF226_13365"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7075024.1"
                     /translation="MLRSGCMLMSVVLLGAAWLSQPLAAREFRISHQWSADVDARDRA
                     ARVFMAEVHKRVPDIKLTIHPKSSLGFKPAEQFDALVDGRLELAIYPLFYGTKKVPEF
                     SIGLLPGVPANTELAGLLKGSEFEEKLQALAMKNGIRILTWWWLGGGIASRSQEIAGP
                     DTVKDISLRTGDKAFDLMVQAAGGDTPSMPSTEIASRMAAGELEGALTSYESFVSFRI
                     YEHAKFATFGGYGIWTSFQPLIMSRTAWESLTPEEQRAFEEAADISDMYFVETQRDAL
                     KTAVDTFRKAGAQVRELSYDEYEAWLKVAKISSWESYQNISPVAADLFSSMLRSFIDS
                     DKRLSAQSSGNEAAQPVSVPQPQRAVQPKATIKQ"
     gene            6152..7543
                     /locus_tag="VF226_13370"
     CDS             6152..7543
                     /locus_tag="VF226_13370"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX7075025.1"
                     /translation="MVRASWSPDSWRSKPIVQVPDYPDSAALSEVEAKLATFPPLVFA
                     GEARQLKKELAEVAAGNAFLLQGGDCAESFAEHRADNIRDFFRVFLQMAVVLTYAGGS
                     PVVKVGRLAGQFAKPRSSPVEKHGHVELPSYRGDIINGAEFTAEARIPDPHRQLQAYR
                     QAAATLNLIRAFAMGGYADLERVHQWTLGFVKDSPQSHRYQELADRITETLVFMRACG
                     ITSETTPQLRTTSVYTSHEALLLGFEQALTRLDSTSGEWYATSGHMLWIGDRTRQPDH
                     AHVEFCRGIRNPIGLKCGPSLDEDGLLRLIDLLNPKNEPGRLTLICRFGADKVESHLP
                     KLIRAVRKEGHSVVWSCDPMHGNTITAASGFKTRPFERILKEVELFFDVHRGEGTHAG
                     GIHVEMTGQNVTECTGGAAAISEADLSDRYHTHCDPRLNADQALELAFLIAERLKQDR
                     TAHIPPVAASAWG"
     gene            7670..9409
                     /locus_tag="VF226_13375"
     CDS             7670..9409
                     /locus_tag="VF226_13375"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX7075026.1"
                     /translation="MSRTFKIALAQLNPVVGDIAGNVEKAKRAHAEAARLGADVVAFS
                     ELFVTGYPPEDLVLKPAFAAAARRGLEELAREVNDGPALLIGTVWAEEGHAYNAVALL
                     DEGAIQAVRYKVDLPNYGVFDEKRVFRAGPLPGPINIRGVRVGVPICEDIWNEEVVEC
                     LEECGAEIIVTPNGSPFDRMKPDLRMNVAVARITECGLPIAYLNQVGGQDELVFDGAS
                     FVLNKDASLAVQLPAWEEAVVLTEWEEVDGSWRCKPGLKAILEEGEAAAYQACMLGLR
                     DYVDKNGFPGVVLGLSGGIDSALVAAICVDALGAERVSSYMLPYRYTSNESLSDAAAC
                     AEALGIRYEILPIEAPVAGFSTALERVFAGREPDITEENIQSRVRGSMLMAISNKFGS
                     MLVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKTQVYKLARWRNAHVPKGGKGPGRI
                     VIPENILTRAPSAELRENQTDQDSLPPYDVLDDILECLVEHEMPLPDIVARGHAPETV
                     KRVERMLYLAEYKRRQAAPGVKITTRSFGRDRRYPITNRFRESVDPAAAVAPATAGGP
                     RMVSPRADGGEVS"
     gene            9468..10247
                     /locus_tag="VF226_13380"
     CDS             9468..10247
                     /locus_tag="VF226_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX7075027.1"
                     /translation="MAELTFQAHALQLAERIDLKGLEREDQLSSNPLAFPVGRDSMAV
                     LFRFGAAVFINVDPLREEEILAGLKPRLVDPLPQRETEAATIVIKPDGDDALVGGNIE
                     LKNAAPERLLLVAEALAVSVALAYDEQRIAAAFDRIEPVASSLKRRKLLRGSRSDLVG
                     QIGEALLIQQRLAGRIEIADRPDVLWDHPELERLWVKLADDYDLAIRARAIGQKLEVI
                     RETADTMADLLSTRTSHRLEWYIIVLILVEILLGLYDRFLK"
     gene            10290..>11539
                     /gene="gltX"
                     /locus_tag="VF226_13385"
     CDS             10290..>11539
                     /gene="gltX"
                     /locus_tag="VF226_13385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7075028.1"
                     /translation="MTVTVRFAPSPTGRLHIGNVRTAVLNWLFARKHKGTFILRLDDT
                     DRQRSTEAFAESIREDLTWLGLTWAREERQSRRTARYDEIAEQLKRAGRLYPCYETEE
                     ELDRRRKRQLARGAPPIYDRAALKLTDEERRKLEAEGRRPYWRFRLENTEPSDPLKPV
                     PTLVDWVDLVRRDQSVDVGSLSDPVMIRTDGTYLYTFTSVIDDIDFGITHVIRGEDHV
                     TNTGVQIQIFQALGAVPPTFGHHSLLVGADGQALSKRLGHLSVAGLRDAGFEPMAILS
                     LAALLGTSEPVEPHQDLDELARLIDFDKISRAPARFDEAELKSINAHLLQHTPYEKVK
                     DRLHAMGIAGGEAFWLAVRGNITYLADASEWWTVVSQPIDPVIEDESFCRRAADLLPE
                     EPWDERTWSAWTSAIGAETGRKGRA"
BASE COUNT         2086 a         3586 c         3703 g         2164 t
ORIGIN      
        1 cgatcagcgc cgcctgcacg agctcgcgcc tcgagatcat gccgctacct cattgtatgt
       61 gttcgaggaa gagctcggtc aggcgaccgt tactttctgc tcgtgcttga agatggtgcc
      121 gtcgtcgtcg agccacgtga actcgaacgt ccccgtctcc ggaaccctga ccgaaaaggc
      181 aagatacggg ttcgccgaaa tcgcgggatg ccaatcgctg tgaaaaattt ccttgccgtt
      241 gaacttggcc gtgaacatct tgatgatctt gcgcgggatc ggcttgcctt gagcgtcctt
      301 gcgctgaccg ctttccattt cgtgcgagat cagcgttttc acttctatga cctcgccggc
      361 tttggccttg cgcggcatac gaacgcgggg tgtagatttt accatgatgt ttcctccgaa
      421 ttctcgcaat cgagctctag ccgccgcatc cgccgatagt gactttcact tcctgctttg
      481 cggaatggag agtgccgtcg gccatctcgg caaccgcaac gacattctgc gtcccggcga
      541 ggcggatgcg aaattgcgca gcagccttcg gcatcaaagg cgtgaagtag aaggacgcga
      601 cgccggggtt cggatttccg tccgcgaata tgtgaaccgc cttgacgtaa tcgttctcgg
      661 tcatcgggct ttccacctcg aacttgagcg gaacgacctg accgttctca gcgatgctcg
      721 gaagctcgag cttgatcttt ccttcggtgc ggggcttgtc gccgtagagc ttcttgagct
      781 cggcctccac ctgctccggt ttcgccattg tcagccgtgg cgccatcacc agggcagccg
      841 cggcggcccc caccgcgatc agcatgctgc gacggctgag cgcgctgcaa gattgttcca
      901 tggacttcct cctgatcaga atgcacgtgc tgcccgcacc gagcatcttg tcacactatg
      961 atatcaaaat gtaaagtgct caagagctgt tgcgtaccat ctcacgatcc tgtcattcgg
     1021 attcaatccg gcatcagcct tcagggagga aaaatgaaac cggtcagaat aagagacttc
     1081 gtgccccttt tggcgccgct cctgctcatg ctggcagcgg caccaggcct tgctcaggaa
     1141 acagtcgaca gcgaaaaggc gatcgagcag taccgccaga tgctcaagga ggacccttgg
     1201 agcaacccgg cgatgctcga tgtcgatcgc ggcgaacagc tctggaaaac gccacgtgga
     1261 ccgaagagcg tttcacttga aggttgcgat ctcggcaaag gccccggagt cctggaaggc
     1321 gcgtttgctg agctgccccg ctacttttcc gatgcggacc gggtcatgga tctggaggct
     1381 cgtatcgtct ggtgcatgga aaagctgcag ggtttcgacc ggaaggagct cgtgacgaag
     1441 ccttatccga aaggcgggag cccaacgaag gacatcggcg caatcgcgac ctttgtcgcc
     1501 tatcagtcga acggactgaa gatctcccca aagctcgagc atgcgaagga gaaggaagcc
     1561 ctggcgcttg gcgaagcgct cttcttccgt cgacagggtc cgatggactt ctcctgcgcg
     1621 acttgccacg cagacaaggg caagcgcatc cgcctgcaga acctgccgta tctgtccgat
     1681 ccatccgagg cacgcaaggt cgtcggcgag tggcccgcct accgtgtctc atcgagccac
     1741 gtcatgacca tgcagcaccg catttacgac tgcttctggc agatgcgtct gccggaggtc
     1801 gagcttggct ctgacgtgcc ggttgcactg acgatgtatc tggccaagat ggctgaaggc
     1861 ggggagatca gcgctccggg cctcaagcgt tgacggggag gaaaacgtga tcagaaatat
     1921 ttgcgcagcc gcaattctcg tattgtcggg gtcgatacct gctttcgccc aatctcccga
     1981 ggtttccgga gaccgcgtgc aggccgccat caaggcgagc tggccgaatc agccgcccga
     2041 gtggcagtcg cgtctcgagc aggacgagac gatgaggctc tgcagccaat acaggaacga
     2101 tcctccatcg gatgtcgccg aggccatcat ggcccgcgag agatcgaaca tcaaatatcc
     2161 ggccgatggc aagttgatgg gcgattggaa gaagggtgaa aagcttgccc aatccggtta
     2221 tgggcgccgt ttcaccgatt atccgccccg gcgcgagaac ggcggcaact gctatgcctg
     2281 ccatcagctc gcaaaggagg aggtgagctt cggcacgctc ggaccgagcc ttctcgagta
     2341 cggaaaaatc cgcgagttca gcgaggccga tgtgaaggcc gtctacgagc gcatttacaa
     2401 tccgcaggcc ttccaggcgt gtgccaacat gccgaggctc ggagcaaacg gccatttgac
     2461 catcgaacag atcaaggatc tcgttgccta cgtcatgtcg ccggacagtc ctgtgaacaa
     2521 atagtcggag gacgcgggag cgacgtgaga atgcgagctc ttgcaggcct catcatatcc
     2581 gctatcgcca gcgttctggt tggcggcagc gcgcttgcgg cggaactcgt catgttcgag
     2641 gatccggcct gcccatattg ccggcgctgg catgccgagg tgggcgtcag ttatcatgca
     2701 actgaggaag gccggatcgc gccgctgcgt cgcgtcgaca tcaaagacag gtacaaggcg
     2761 ggcgtatctc taaaaggccc ggtcacgttc acgccgactt tcgtgctggc cgaggatggc
     2821 cgcgaggtgg gccggatcgt cggctattcg ggagctgatt tcttctacgg tttcctggca
     2881 aacctgctcg agcagcgtgc cagcagcccg gcacgaacaa ggggtctttc gcggtgagcg
     2941 agcgtgcgat atggctgtga gagccgtata tgtatgatga tcgacgtcaa tcgatggcat
     3001 ccgtgggcac gaacgaatca gatcgagcat aaatgagccg gatcctttcc aataaggtcg
     3061 tcgaggcgct tcagagcgac gtcgagctgt cgcctgagct cacccgcatc atgaagaacg
     3121 cccgcgcggc gagtgatttt ctcaaggcgc tggcgcatga gcacaggctg atcctgctgt
     3181 gcctgctcgc cgagcgggag cgctcagtca ccgagctcga ggagcttctg tcccttcggc
     3241 agaccacggt ttcccagcag cttgcgcggc tgcgtttcga tggcatcgtg accacgcgca
     3301 gggtcggcaa agtcgtctac tacagtctcg ccgatacgag cacgttgaag ttcgtgaagg
     3361 tgatctacga tatgttctgc tcagacccca aacccgaaaa agactcggcc tgagcagggc
     3421 ggcgaagtcg tttcaagatc gcaaacgccg ccatttggag aggaagcgct gctgtggacg
     3481 ggcttattgc gccggtcggc ggcttcattt gcggaggtct cgccgggttc gctgcacagt
     3541 atggacgact gtgcacgatc ggcgccatgg aagatgctct cgtggcgggc gatttccgcc
     3601 gcgccggggc gtgggccctg tcggccgcgg tcgccgttct gcttacgcag ggcctcgtgt
     3661 ttgccggcgc gctcgagctt tcgggcatcc actacgtccg tcccatgctc gacatcaccg
     3721 gattggtgat tggaggtctg ctgtttggct tcggggcagc gctggtgggc acgtgtgctt
     3781 ttggcctcct cgtgcgcctc ggaactggtg atctgcgcgc gctggtttgc gccctcgtcg
     3841 ttggtctggc ggcattctcc gcgacaggcg ggattctcag ccccctgcgg ttggccctgt
     3901 cggagttcgg cgctctcgat acgtctgcca tcggcggcaa gacactcacc ggcatcatcg
     3961 gcgccagccc gcacagcgct gcttcctttc tggctgttct gctcgtcgca ggtgctctgc
     4021 tggtattcgc tctgacgaat tggcggctct accgcaaacc gcgcctgatc gccgcctcct
     4081 gcctgatggg cctcgccgtc gcagggggct ggctgaccac gggcgtgctt gccgacccgt
     4141 tctccggcca gcgcccggaa agcctcacgt tcgtcatacc tcttggccgg ctccttctgc
     4201 agctggtggg cgaaagacta tcgagcaccg agttcgcagt ggcgacggtt ttcggcgtca
     4261 gtgccggctc gttcgcggtc gccgcggttc gcgacgagct gcgctgggag gcgttcgacg
     4321 atcaccgcga gatgcgccgc cacctcctcg gcgcactcct catggggttt ggcggcgtcc
     4381 tggcccgcgg ctgcaccata ggccaggggt tgaccgggat gagcgtactg tcgcttgcag
     4441 caccggtcgc agtctttgcg atggtggtgg gggcgagggc ggggctggtg tatctcgtgg
     4501 aagggcggcg actgtttggg caacgctatt gaccgcgcca ccgggcggcg ctggcaaacc
     4561 tgggttgccc gtcccagata cgggggtgcc cttcccggat tcgaggtccg aggccgaaca
     4621 gcgtgcgggc cgctcgagcg gatcgatttc tccctccctc ccacctgtgg agagggaggg
     4681 gaatgtgacg gcccgaccgc ccgggacgcc tattgcttga tggtcgcctt gggctgaacc
     4741 gcgcgctgcg gctgtgggac ggagacgggc tgtgccgcct cgttgcccga gctttgggcg
     4801 gatagacgtt tgtcgctgtc gatgaagctg cgcagcatcg acgagaagag atccgccgcg
     4861 acagggctga tgttctggta actctcccag gagctgatct tcgcgacctt cagccaggcc
     4921 tcgtactcgt cgtagctcag ctcacgaacc tgcgcgcccg ccttcctgaa ggtgtcgacg
     4981 gcggttttca gcgcatcccg ctgagtctcg acgaaataca tgtcagagat atctgctgct
     5041 tcctcgaacg cccgctgctc ttccggcgtg agcgattccc aagcggtgcg cgacatgatc
     5101 agcggctgga acgaggtcca gatcccgtac ccaccgaacg tcgcgaactt ggcgtgctcg
     5161 tagatgcgga acgagacgaa cgattcatac gacgtcaggg caccctccag ctcgcccgcg
     5221 gccatccggc tggcgatctc ggtcgacggc atcgacggcg tatcaccgcc ggcagcctgc
     5281 accatgaggt cgaaggcctt gtcgcccgta cgcaacgata tgtccttcac cgtgtcagga
     5341 ccggcgatct cctgcgagcg gctcgcgatg ccgccaccga gccaccacca ggtcaggatg
     5401 cggatgccgt tcttcatggc gagcgcctgg agcttctcct cgaactccga gcccttgagg
     5461 agaccggcga gctcggtatt ggcgggtacg ccgggcagca ggccgatcga gaactcgggt
     5521 actttcttgg tgccgtagaa aagcgggtag atggcgagct cgagccggcc atcgaccaga
     5581 gcatcgaact gctccgccgg cttgaagccc agcgaggact tcggatggat ggtgagcttg
     5641 atgtcaggaa cccgtttgtg cacttcggcc atgaagacgc gagccgcgcg atcgcgcgca
     5701 tcgacgtctg ccgaccactg gtgagagata cgaaactcgc gcgccgcaag tggctgtgac
     5761 agccaagctg ctccgagcag caccacactc atcaacatac agcccgagcg cagcatggac
     5821 gaaatccccc cgtttaggct ccggatgcta caacaacgat ggatccggtg accttagcca
     5881 ccgtcgcctc aacccaatgc catgccacct cgtcgattcc aaggcggagg actgattcag
     5941 catcaacgat atcaggtgac tgaggttcaa atgccagcca gccggacgga gggcgccccg
     6001 gccgagcgac gcaacgaagc gcgtgctggc ctgacgcatc ggattggctt ataagcgcca
     6061 cacatctaat aaacctttcc tgtcccggtc cccgaagttc gccaggactg gtgcacggcg
     6121 cgaaagctcg agcgatatca ggagattgtc gatggtgcgg gcgtcctggt cacccgacag
     6181 ctggaggtcg aagccgattg tccaggttcc cgactatcca gacagcgctg cgctgtctga
     6241 ggtggaggcc aagctcgcca ctttcccacc gctcgttttt gcaggtgaag cccgtcagct
     6301 gaagaaggag ctggcggagg tcgccgccgg caatgcattc ctgttgcagg ggggcgactg
     6361 cgccgaaagc ttcgccgagc accgggccga caacatccgc gacttcttcc gagtcttcct
     6421 gcagatggcg gtcgtgctga cgtatgccgg cggctcgccc gtcgtcaaag tgggacgcct
     6481 cgcgggccag ttcgcgaaac cacgctcctc gcccgtcgag aagcacgggc acgtcgagct
     6541 gccgagctat cggggcgata tcatcaatgg tgccgagttc acggccgagg cacgcatccc
     6601 cgatccgcac cggcagcttc aagcctaccg gcaggctgcc gccacgctca acctgatccg
     6661 cgccttcgcc atgggcggct acgcggacct ggagcgggtc catcagtgga cgctcggctt
     6721 cgtcaaggat tcaccgcaga gccaccgcta tcaggagctc gccgatcgca tcacggagac
     6781 gctggtattc atgcgggcgt gcgggatcac gtcggagacc acgccgcagc ttcggacgac
     6841 cagcgtctac acgagccacg aggccctgct gctcggcttc gagcaagcgc tgacgcggct
     6901 cgattccacc agcggcgagt ggtacgccac ctcgggccat atgttgtgga tcggtgaccg
     6961 cacccgccag ccggatcacg cccatgtcga gttctgccgt ggcatccgca acccgatcgg
     7021 gctgaagtgc ggaccgtcgc tggacgagga tggactgctc cggctcatcg atctgctcaa
     7081 tccgaagaac gagccgggac gactgacact gatatgccgg ttcggcgcgg acaaggtcga
     7141 atcgcatctg ccgaagctca tccgggccgt gcgcaaggag gggcattcgg tcgtgtggtc
     7201 ctgtgatccg atgcacggca atacgatcac cgctgcctcg ggcttcaaga cgcggccgtt
     7261 cgagcgcatc ctcaaggagg tcgagctgtt cttcgatgtg caccgtggcg agggcactca
     7321 cgccggcggg attcacgtcg aaatgacggg ccagaacgtg accgaatgca caggcggcgc
     7381 cgcggcgatc tcggaagccg atctgtccga tcgctaccac acgcactgcg atccgcggct
     7441 caatgccgac caggcgctcg agcttgcgtt tctgatcgcc gagcgactga agcaagaccg
     7501 gacggctcat attccgcccg ttgcagcgag tgcatgggga tgagagtagc gaatagtggg
     7561 tagcgaatgg cgaattgaac cctattcgct gctcgccgtt cgctactcgc caatccgctg
     7621 ctcgctcgcc gtccttccct tcctccaccg gatctctaag tactgaggca tgtcgagaac
     7681 cttcaagatc gcgctggctc agctcaatcc ggtggtcggc gatattgcgg gcaacgtcga
     7741 aaaggcaaag cgcgcccatg cagaggcagc ccgcctcggg gcggacgtcg tcgctttttc
     7801 cgagctgttc gtgaccggct atccgccgga ggacctggtc ctgaagccgg cgttcgcggc
     7861 cgcggcgcgc cgggggcttg aggagctggc ccgcgaggtg aacgatgggc ctgcgcttct
     7921 gatcggcacc gtctgggccg aggaggggca cgcctacaat gccgtggcgc tgttggacga
     7981 gggcgccata caggcggtcc gctacaaggt ggatttgccg aactacggtg tgttcgacga
     8041 gaagcgcgtg tttcgcgcgg ggcccctgcc cgggcccatc aacattcgcg gcgtccgcgt
     8101 cggcgtgccg atctgcgagg acatctggaa cgaagaggtg gtcgagtgcc tcgaggagtg
     8161 cggggcggag atcatcgtga cgcccaacgg ctcacccttc gaccggatga agcccgatct
     8221 caggatgaac gtcgccgtcg cgcgcatcac cgagtgcggc ctgccgatcg cctacttgaa
     8281 ccaggtcggc gggcaggatg agctggtttt cgacggcgcc tcgttcgtgc tgaataagga
     8341 cgccagtctg gccgtccagc tcccggcctg ggaggaggcg gtcgtcctca ccgagtggga
     8401 ggaggtcgac ggaagctggc gctgcaagcc cggcctcaaa gccattctcg aggagggcga
     8461 ggcggctgcc tatcaggcct gcatgctggg cctgcgcgac tacgtcgaca agaacggctt
     8521 cccgggtgtc gtgctcggcc tctcgggcgg catcgactcc gcgctggtgg ctgcaatttg
     8581 cgtcgacgcg ctgggcgcgg aacgggtgtc ctcctacatg ctgccctatc gctatacgtc
     8641 gaacgagagc ctttccgacg ctgcggcctg cgcggaagcc cttggaattc gatacgaaat
     8701 tctgccgatc gaggctcctg tcgcggggtt ctcgacggct ctggagcggg tcttcgccgg
     8761 ccgcgagccg gacatcaccg aggagaacat ccaaagccgt gtgcgcggct cgatgctgat
     8821 ggcgatctcc aacaagttcg gaagcatgct ggtgacgacc ggcaacaagt ccgagatgtc
     8881 ggtaggctat gccacgctct acggggacat gaacggcgga ttcaatccga ttaaagacct
     8941 ctacaagaca caggtctata agctggcccg ctggcgcaat gcccacgtcc ccaagggcgg
     9001 caaggggccg ggccgcatcg tgatccctga gaacatcttg acgagggcgc cctcggcgga
     9061 gctgcgcgag aaccagacgg atcaggattc gctgccgccc tacgacgtcc tggacgacat
     9121 tctcgaatgc ctcgtcgagc acgagatgcc gctgccggac atcgttgccc gaggccatgc
     9181 gccggagacg gtgaagcgtg tggagcgcat gctctatctc gccgagtaca agcggcgcca
     9241 ggcggctccg ggcgtgaaga tcacgactcg cagtttcggc cgcgatcgcc gttatccgat
     9301 caccaacagg ttccgcgaaa gcgtagatcc tgctgccgcc gtggcaccgg cgaccgccgg
     9361 ggggccgcgg atggtttctc cgcgcgccga cggaggcgag gtgtcttagg gcctgcaggc
     9421 cgggtatcgt ttgcgatgca gtcgcttctg ttcagcccga ggcgtgcatg gcggagctga
     9481 cgtttcaggc gcacgccctg cagcttgccg agcgcatcga tctcaagggg ctggagcgcg
     9541 aggatcagct ttccagcaat ccgctcgctt ttccggtcgg ccgggacagc atggccgtgc
     9601 tgtttcgctt cggggcggcg gtcttcatca atgtcgaccc gcttcgggag gaggagatct
     9661 tagccgggct gaaaccgcgg ctcgtcgatc cgttgccgca gcgcgagacc gaggccgcga
     9721 cgatcgtcat caagccggat ggcgacgatg cgctggtcgg aggcaacatc gagctgaaga
     9781 atgcagcgcc ggagcggctg ctgctcgtcg ccgaggcgtt ggcggtcagc gtcgcgctgg
     9841 cgtatgacga gcagcgcatc gcggcagcgt tcgaccgaat cgagccggtc gcgagcagtc
     9901 tgaagaggcg caagctgctg cgcggcagcc gctcggatct cgtcgggcag atcggtgagg
     9961 cgctgctcat ccagcagcgg ctcgccggcc gcatcgagat cgccgatcgg ccggacgtgc
    10021 tttgggacca tccggagctc gagcggctat gggtgaagct tgcggacgat tatgatctcg
    10081 caatacgtgc ccgggcgatc ggccagaagc tcgaggtgat ccgcgagacg gccgacacga
    10141 tggcggatct gctgtcgacc cggacgagtc accgcctcga gtggtacatc atcgtattga
    10201 ttctggtcga gatcctgctc ggcctctatg accgctttct gaaatgagcg atgcacgccg
    10261 ctcgatgcgt gcaggagagc tgtaccggca tgaccgtcac cgtccgtttc gccccaagcc
    10321 ccaccggccg cctgcacatc ggcaatgtcc gcacggccgt gctcaactgg ctcttcgccc
    10381 gcaagcacaa gggcacgttc atcctgcggc tcgacgacac cgatcgccag cgttcgaccg
    10441 aggcgttcgc cgaatccatc cgcgaggacc tgacctggct cggtctcacc tgggcgcggg
    10501 aggagcggca gtcgcggcgg accgcccgct acgacgagat cgccgaacag ttgaagcggg
    10561 ccgggcggct ctatccctgc tatgagacgg aggaggagct tgatcgccgc cgcaagcgcc
    10621 agctcgcgcg gggtgcgcca cccatctacg accgggccgc attgaagctg accgacgagg
    10681 agcgccgcaa gctcgaagcc gaagggcgac gtccctactg gcgcttccgg ctggagaaca
    10741 ctgagccttc cgatccgctc aagccggtgc cgacactggt ggactgggtc gatctcgttc
    10801 gcagggatca atcggtcgac gtcggatcgc tgtccgaccc ggtgatgatc cggaccgacg
    10861 gcacctacct ctacaccttc accagcgtga tcgacgacat cgatttcggc atcacccacg
    10921 tgatccgggg agaggaccac gtcaccaaca ccggcgtgca gatccagatt ttccaggcgc
    10981 tgggtgcggt gccgccgaca ttcggccatc actctctcct ggtcggcgca gatggacagg
    11041 cgctttcgaa gcggctgggg cacctctccg tcgccggtct gcgggatgcc ggcttcgagc
    11101 cgatggccat actgagcctt gccgcattgc tcggcacctc ggagcctgtc gagccgcatc
    11161 aggacctgga cgagctcgcc cgtctgatcg atttcgacaa gatttcgcgc gcgccggccc
    11221 ggttcgacga ggcagagctg aaatcgatca acgcgcatct cctccagcac acgccatatg
    11281 agaaggtgaa ggatcggcta cacgcaatgg gcatcgcggg tggcgaggcg ttctggctgg
    11341 ccgtgcgcgg caacatcacc tatctcgccg acgcgtcgga atggtggacc gtggtgtcac
    11401 agccgatcga cccggtcatc gaagacgaga gcttctgccg gcgcgcggct gatttgctcc
    11461 ccgaggaacc ctgggacgag cgaacctggt cggcctggac gtcagccatt ggcgccgaaa
    11521 ccggccgcaa gggccgcgc
//
LOCUS       DASVDU010000209         7661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1183087, whole genome shotgun sequence.
ACCESSION   DASVDU010000209 DASVDU010000000
VERSION     DASVDU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183087"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1802
                     /locus_tag="VF226_13390"
     CDS             <1..1802
                     /locus_tag="VF226_13390"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7075029.1"
                     /translation="GRRHNLPMINIFDAEARINENAPEAYRGLDRFEARKRVIADLEA
                     QGHVEKIEPYTHMVPHGDRSAVPIEPWLTDQWYVNAAELAKEAIKAVEDGRTKFVPKN
                     WEKTYFEWMRNIQPWCISRQLWWGHQIPAWYGPDGEVFVEETENLALAAAQRHYGTRV
                     NLERDPDVLDTWFSSALWPFSTLGWPDKTPELERYYPTDVLVTGFDIIFFWVARMMMM
                     GLFFMKEVPFRDVYIHALVRDEKGQKMSKSKGNVMDPLELIDAYGADALRFTLAAMAA
                     QGRDIKLARSRVEGYRNFATKLWNAARFAEMNECVRVKGFDPTSAKLTVNRWIAGETE
                     RTAKAVTAAIEAYKFNEAASAIYEFTWGVFCDWYLELVKPILGGSDEGAKSETRAMVA
                     WALDQVLKLLHPFMPFITEELWAHMVEHGIKRDAMLALSPWPELDGLADPAADEELGW
                     VVNLVSEVRSVRAEMNVPAGARVPLVLVGSNDAIRRRAEHHEETISRLARLGSISFED
                     APPAGAAVIAFGETTAALPLSGIIDMAAEKARLRREIDKATADIGKIDAKLANAQFLA
                     KAPEEVVEEQRERKAELETLSARLANALKRLEG"
     gene            2647..3582
                     /locus_tag="VF226_13395"
     CDS             2647..3582
                     /locus_tag="VF226_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7075030.1"
                     /translation="MSALRAGQLDVALPALEHAASEGIFLAEFYLARIFADPTSGYTD
                     HAKAYVLYQRLADQYADIDPDDDQRAPFVAKAFTALAGYLLRGVPELNVKPNPSRAAE
                     YLHHAATFFNDHDAQFELAKLYLRDDDEPIKTRQALHWLSVLTEQGHAGAQAFLADLY
                     WHGNHVEKSPLRAYALVTVAVENAPPHERIWIEDIHQNIYCGLSETVRTNAKGLIAFW
                     RKQYGRTPEADRYGLSRLQIGPVRTCSNGEVVPVPGQTRSVVASEPTSKAATGDVATS
                     ALQTQKRPAGPTVAQGNPAGFGVRDAREVVTEQHN"
     gene            complement(3619..4404)
                     /gene="xth"
                     /locus_tag="VF226_13400"
     CDS             complement(3619..4404)
                     /gene="xth"
                     /locus_tag="VF226_13400"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX7075031.1"
                     /translation="MKIATWNINGIKARLDTALTWIADAKPDVVCLQEVKCMDGAFPS
                     GPFESLGYNIAVHGQKGFNGVAILAKSRFDDVRPGLPGAPDDVQARYLEVTLSIPSGA
                     VTIASIYLPNGNPIGTEKFDYKLAWMDRLSQRARVLLADEQPLVLAGDFNVIPEPLDA
                     KNPENWANDALYQPETRARYRALVNGGFTDAIRACHPGPNVFSFWDYQAGAWQKDDGI
                     RIDHMLLSPQAADRLLVAGIDRHTRGWEKPSDHVPVWLELDVG"
     gene            4568..4918
                     /locus_tag="VF226_13405"
     CDS             4568..4918
                     /locus_tag="VF226_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075032.1"
                     /translation="MGLSLYESKRRDPKPLVALMAANLLFAASSIFLWTLMSLFIAEP
                     VRWATARGIGSRPELLEYPFILLWLLPVAGACAAWVALKAERLRLAYLLAVKPLLYFG
                     VVAGWYYLAPAQWH"
     gene            5022..6650
                     /locus_tag="VF226_13410"
     CDS             5022..6650
                     /locus_tag="VF226_13410"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7075033.1"
                     /translation="MQRYIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVPAKRTDNITTGRIYQDILAKE
                     RRGDYLGATVQVIPHVTDAIKEFITSGNEGIDFVLVEIGGTVGDIEGLPFFEAIRQLG
                     NELPRGSVVYIHLTLLPYIPSAGELKTKPTQHSVKELRSIGIQPDILLCRSDRPIPRS
                     ERKKIALFCNVREEAVIQALDVSSIYDVPLAYHSQGFDREVLAAFGMNDAPKPDLTRW
                     EAISDAIANPEGEVTIAVVGKYTELKDAYKSLIEALVHGGIANKVRVNIDWIESEIFE
                     REDPALFLEDVHGILVPGGFGERGAEGKILAARFARERKVPYFGICFGMQMACIEAAR
                     NLAGIPEASSTEFGPTSEPVVGLMTEWMRGDQREKRNAGGDLGGTMRLGAYEAHLKGN
                     SRIAATYGTTIISERHRHRYEVNPKYRERLEEAGLIISGMSPDGLLPETIEYPDHPWF
                     IGVQFHPELKSRPFDPHPLFKGFIAAAIEQSRLV"
     gene            complement(6683..7024)
                     /locus_tag="VF226_13415"
     CDS             complement(6683..7024)
                     /locus_tag="VF226_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX7075034.1"
                     /translation="MARRPGQPQSRRAPRAKAGQKRNPRYWSQEVTRASNALDLEPGV
                     FTWDDPVRIAQSLKQSAETSTRRKGTPLQSAMSMLNFYINRAGRNLPADRRQVLERAK
                     AELRRAFKSAA"
     gene            7180..7644
                     /locus_tag="VF226_13420"
     CDS             7180..7644
                     /locus_tag="VF226_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX7075035.1"
                     /translation="MSTRGHCLCRAIIYEFDGEPKWVAYCHCESCRRATSAPVTTYVG
                     VPLDRFRYLQGKPAVYESSPGVRRSFCPRCGSPMAYEADRYPGEIHLHICTLADPNRF
                     APRAHVFAGEQLTWFEIADELRRYETLGGKGVAPLRVGPRPAPPAASPGGEA"
BASE COUNT         1364 a         2420 c         2455 g         1422 t
ORIGIN      
        1 tcggccgccg gcacaacctg ccgatgatca acatcttcga cgccgaggcg cggatcaacg
       61 agaacgcgcc ggaggcctat cgcggcctcg accgcttcga ggcgagaaag cgggtgatcg
      121 ccgacctcga agcgcagggg catgtcgaaa agatcgagcc gtacacgcac atggttcccc
      181 acggcgaccg ctcggccgtg ccgatcgagc cgtggctgac ggatcagtgg tacgtcaatg
      241 cggccgagct ggcgaaggag gccatcaagg ccgtcgagga cgggcgcacg aagttcgtcc
      301 ccaagaactg ggagaagacc tacttcgagt ggatgcgcaa catccaaccg tggtgcatct
      361 cgcgccagct ctggtggggg caccagatcc cggcctggta cggtccggac ggtgaggtgt
      421 tcgtcgagga gacggagaac ctggctctgg ccgcagccca gagacactac gggacgcggg
      481 tcaatctcga acgcgatccc gacgtgctgg acacgtggtt ctcgtccgct ctctggcctt
      541 tctcgacgct cggctggccc gacaagacgc cggagcttga gcgctattat ccgacggacg
      601 tccttgtcac cggcttcgat atcatcttct tctgggtcgc ccggatgatg atgatgggcc
      661 tcttctttat gaaagaggtg ccgttccgcg acgtctacat ccatgccctc gtccgtgacg
      721 agaaggggca gaagatgtcg aagtccaagg gcaacgtcat ggaccccttg gagctgatcg
      781 acgcttacgg cgccgacgcg ctgcgcttca cgctggcggc catggcggcg cagggccgtg
      841 acatcaagct cgcccgatcg cgcgtcgagg gataccggaa cttcgcgacc aagctctgga
      901 acgccgcgcg ctttgcggag atgaacgagt gcgtgcgcgt caagggtttc gatcccacct
      961 cggcaaagct gaccgtgaac cggtggatcg cgggcgagac ggagcggacg gccaaggctg
     1021 tcaccgctgc gatcgaagcc tacaagttca acgaggccgc gagcgcgatc tacgagttca
     1081 cctggggcgt cttctgcgac tggtacctgg agctggtgaa gcccatcctc ggcggttccg
     1141 acgaaggagc caaatccgaa acgcgcgcca tggtggcctg ggcgctcgac caggtgctga
     1201 agctgctgca tccgttcatg ccattcatca cggaggagct gtgggcgcac atggtcgagc
     1261 acggcatcaa gcgcgatgcc atgctggcgc tgagcccctg gcctgagctc gacggacttg
     1321 ccgacccggc tgcggatgag gagctcggct gggtcgtcaa tctcgtcagc gaggtgcgct
     1381 ccgtccgtgc tgagatgaac gtgccggccg gcgccagggt gccgctcgtg ctggtgggca
     1441 gcaacgatgc catccggcgc cgggccgagc atcacgagga gacgatcagc cgacttgcac
     1501 gcctgggctc catctcgttc gaggacgcac cgccggctgg tgcggcggtc atcgccttcg
     1561 gtgagaccac ggccgccctt ccactttcgg gcattatcga tatggccgcg gagaaggcgc
     1621 ggctccggcg tgagatcgac aaggcaactg ccgacatcgg caagatcgac gccaagctcg
     1681 ccaacgcgca gttcctcgcc aaggcgccgg aggaagtcgt cgaggagcag cgggagcgca
     1741 aggcggagct cgaaacgctc tcggcacggc tggccaacgc gctgaagcgc cttgaaggat
     1801 gatgggagcg aatggcgagt agcgaatagc gaatcggccg ctgtagtttc ctgtgcgcca
     1861 gtcgcatttt cgccatttgc tcttccggta acctcccatt caccactgtg gcgttgtggc
     1921 aacgaaagcg ggcgttattg cctcttttga gccacaaaga ccgaccacga tgcatgaaca
     1981 tcagatgaat gcggccgtcc actctctgga gctttgttcc agtggcggag tggtggatcg
     2041 gatgggatca acttaacgaa gattggcctg cctggagtgc gagcgcgcgt gcgcatgcga
     2101 actcacgaga cgctctcctc gcaggaagcc gggtcgggat ggacgccaga acgctgaacg
     2161 agtccagttc gacgacggag gacgccttgc gtccgcccca atcctgcggc ggcgcagcgc
     2221 ggttctcaga gggctggcgg tttcctgatc cgatcggcgg gtttggcccc ggcagtatca
     2281 gtgcctttgg agttggatct ggcggttcgc acttggtggt gcacaagggt gacaccaata
     2341 tcgcagaatc cggaaagggt acgcgaagcc actttgtgga ccaatccagg ctcgaatctc
     2401 agcgtaatgc gtggacttct cccgcaagtc catacaaaag cggggcgttg aggaagtatg
     2461 cggatcagat tgggctgacg gatgtcagtt ctgtcgccca ggctggcgga aaggctgaag
     2521 ttgtctgtct cgctgctatc tagattgctg gtcggcttgg cgttcttcgc tgccctaggg
     2581 cttcaacccg ccctcgcctc ccgcagcgcg agtttcagct ccccgcaggc ggcctttgag
     2641 caggggatga gtgctttgag agccggacag ctcgacgttg ctctccctgc tctcgaacat
     2701 gctgccagcg aaggcatctt ccttgccgag ttttacttgg cacggatatt tgccgatccc
     2761 acgagtgggt atacggacca cgccaaggcc tacgtgcttt accagcgcct tgccgatcaa
     2821 tatgccgaca tcgatcccga cgacgatcaa cgggcgccgt tcgtggccaa agcgttcacg
     2881 gcccttgcgg gctacctgtt gcgcggcgtg cctgagctca acgtcaaacc caacccgagc
     2941 cgcgccgccg agtatctgca tcacgcggcc accttcttca acgaccatga tgcgcaattc
     3001 gagctggcga agctttacct ccgggacgac gatgagccca tcaagacgcg gcaggctctg
     3061 cattggctgt cggtcctgac cgaacagggg cacgctggag cgcaggcgtt cctggcggac
     3121 ctttattggc acggcaatca cgtcgaaaag agccctctcc gcgcctatgc tctggtgacc
     3181 gtggccgtcg agaacgcgcc accgcacgag cgcatctgga tcgaggacat ccaccagaat
     3241 atctattgcg gcctgtcgga gacggtgcgc accaatgcca agggcctgat cgcgttctgg
     3301 cgcaagcaat atgggcgcac acccgaggcg gaccgttatg gcctgtcgcg gctgcaaatc
     3361 gggcctgtcc gcacctgcag taacggtgag gtcgtacccg ttccgggcca gacgcgctcg
     3421 gtcgtggcct ccgagcccac ctcgaaagct gcgacgggcg acgttgcgac gtctgcactc
     3481 cagacacaga agaggccagc cggaccgacg gttgctcaag gcaatcctgc cggctttggc
     3541 gtgcgggacg cccgcgaggt cgtcaccgag cagcacaact gagctgacag cacgcgttgg
     3601 tcgtgcgctt cgcgctcgtc agccgacatc gagctccagc cagaccggca cgtgatcgga
     3661 tggcttctcc cagccgcggg tatgccggtc gatgccggcc acgagcagtc tgtccgcggc
     3721 ctgcggtgac agcagcatgt gatcgattct gatgccgtca tccttctgcc aggcgcccgc
     3781 ctgatagtcc cagaacgaga agacgttcgg tcccgggtga caggcgcgta tggcatccgt
     3841 gaagccgccg ttcaccagcg ccctgtagcg cgcccgcgtc tcgggctgat agagcgcatc
     3901 gttggcccag ttctccggat tcttggcatc gagcggctcg gggatcacgt tgaaatcgcc
     3961 agccagcacc agcggctgct cgtcggcgag gagcacgcgc gcccgctgtg acagccggtc
     4021 catccaagcg agcttgtaat cgaatttttc cgtcccgatg gggttgccgt tcggcaagta
     4081 gatggaggca atcgtgacgg cacccgatgg gatcgacagc gtcacctcga gatagcgcgc
     4141 ctggacgtca tccggtgcgc cgggcaggcc tgggcggacg tcatcgaaac gggacttggc
     4201 gagaatggcg acgccgttga aacccttctg gccgtggacc gcgatgttgt agccgaggct
     4261 ttcgaacggg cctgacggga aggcgccgtc catgcacttc acctcctgga gacacaccac
     4321 gtcgggtttg gcatccgcga tccacgtcag cgccgtgtcg agccgcgcct tgatgccgtt
     4381 gatgttccag gtggcgatct tcatcatcat gccgggaccg gcatccctcc gagccgtcca
     4441 gcatcggcca tcggctcgca ctttctgccg gcgattcccg ccccttgtca gcgaagtgtg
     4501 ttcaccgccg attagcagct tttgatctat cctgctaacc gacactattc gtttcaatgg
     4561 cgcaatcatg ggcctgtctc tctacgagag caaacggcgc gaccccaaac cgctggtggc
     4621 gctcatggcg gcgaacctgc tgttcgctgc atcctcgatc ttcctgtgga cgctgatgtc
     4681 gctcttcatc gctgaacccg tgcgctgggc gacggcgcgg ggtatcggct cgcggcccga
     4741 gctgctcgag taccccttca tcctcctctg gctgctgccg gtggctggtg catgcgcggc
     4801 ctgggtggcc ctgaaagccg agcggttgcg gctcgcctat ctcctggcgg tcaagccgct
     4861 gctctatttc ggtgtggtcg ccggctggta ctatctcgct cccgcccagt ggcattgagg
     4921 ccgccagcct gcaaccaggc gtttagcctc ggccttagcg caagtggatg agacgagatg
     4981 gccgcttcga atcggcctgc ttccgtgtat tctgcaagcc catgcagcgg tacatcttca
     5041 ttaccggcgg cgtggtctcc tccctcggca aaggcctcgc atcggcggct cttggggcgc
     5101 ttctccaggc tcgcggctat tccgttcgcc tgcgcaagct cgacccttat ctcaacgtcg
     5161 atccgggcac catgagcccg tatcagcacg gcgaggtgtt cgtcaccgac gacggcgcgg
     5221 agacggacct cgacctcggc cactatgagc gcttcactgg cgtgccggcg aagcgcaccg
     5281 acaacatcac cacggggcgg atctaccagg atatcctcgc caaggagcgc cgcggggact
     5341 atctcggcgc gaccgtgcag gtcattccgc acgtcacaga cgccatcaag gagttcatca
     5401 cctccggcaa cgagggcatc gacttcgtcc ttgtcgagat cggcggcacg gtgggcgaca
     5461 tcgagggcct gccattcttc gaagccatcc gccagctcgg caacgagctg ccgcgcggct
     5521 ccgtcgtcta catccatttg acgctgctgc cctacatccc cagtgcaggc gaactcaaaa
     5581 ccaagccgac ccagcactcg gtcaaggagc tgcgctccat cggcatccag ccggacattc
     5641 tgctgtgcag gtcggaccgg cccatcccgc gctcggagcg gaagaaaatc gcgctgttct
     5701 gcaacgtgcg ggaagaggcg gtcatccagg cgctcgacgt gtcctcgatc tacgacgtgc
     5761 ccttggccta tcacagccaa gggtttgatc gggaagtgct ggccgctttc ggcatgaacg
     5821 acgctccgaa acccgacctc acgcggtggg aagccatcag cgatgccatc gcaaatccgg
     5881 aaggggaggt gacgatcgcg gtcgtcggca agtacacgga gctgaaggac gcctacaaat
     5941 cgctcatcga agcgctcgtc cacggcggta tcgccaacaa ggtcagggtc aacatcgact
     6001 ggatcgaaag cgagatcttc gagcgggagg acccggcgct gttcctcgag gacgtgcacg
     6061 gcattctggt gccgggcggt ttcggggagc gcggtgcaga gggcaagatc ctcgccgccc
     6121 gctttgcccg cgagcgcaag gtgccttatt tcggcatctg cttcggtatg cagatggcct
     6181 gcatcgaggc ggcccgtaac ctggccggca tcccggaggc gagctcgacc gagttcgggc
     6241 cgacctcaga gcctgtcgtc ggcctgatga ccgagtggat gcgcggtgac cagcgcgaaa
     6301 agcggaatgc cggcggtgat ctcggcggca ccatgcgact gggcgcctac gaagctcacc
     6361 tcaaggggaa tagcaggatc gccgcgactt acgggacgac catcatctcc gagcggcatc
     6421 gccatcgcta tgaggtcaat ccgaaatatc gcgagcgtct ggaagaggcg gggctgataa
     6481 tttcaggcat gtcgcccgat ggattgctgc cggagacgat cgagtatccg gaccacccct
     6541 ggttcatcgg tgtccagttc cacccggagc tgaaatcgcg gccgttcgat cctcatccgc
     6601 tgttcaaggg cttcatcgcc gctgcgatcg agcagtcgcg gctggtttga tccaaaccgc
     6661 gctccttcgc gccatagctg cattaggcag cgcttttgaa agcgcggcgg agctctgcct
     6721 tggcgcgttc gagaacttga cgcctgtccg caggcagatt gcgtccggcg cggttgatgt
     6781 agaagttcag catcgacatg gcggattgca gcggcgtgcc cttgcgccgc gtactggttt
     6841 ctgccgattg cttaagggat tgcgcgatcc tcacgggatc atcccaggtg aagacgcccg
     6901 gctcgaggtc gagcgcattg ctggcgcggg tgacctcctg cgaccagtag cgcggattgc
     6961 gcttctggcc agctttggcg cgaggagcgc gccgggattg tggctgacca ggtcgtcgtg
     7021 ccatcggctc tgcacaaagc ttcaatcact cgtttcccaa cgaacggcgg ccggccagcg
     7081 ttccgaggca ggggttggcc tggcgtgttc gcatcggacg cttcccacgc tgcacgtctg
     7141 gtgtaagctg cggcggcgct tcagaccggg aggaagcgaa tgtcaacgcg tggccactgc
     7201 ctgtgccggg ccatcatcta cgagttcgat ggcgagccca agtgggtggc ctattgccac
     7261 tgcgaaagct gtcggcgtgc gacgtccgcg cccgtgacga cctacgttgg cgtcccgctc
     7321 gatcggtttc gctacctgca ggggaaaccg gccgtctacg agtcctcacc cggggtccgg
     7381 cgctctttct gcccgcgctg cggatcgccg atggcgtatg aggccgaccg gtaccccggc
     7441 gagatccatc tgcacatttg cacgctggcc gatccgaacc ggttcgcgcc ccgcgcgcac
     7501 gtctttgccg gcgaacagct cacctggttc gagatcgccg atgagctgcg tcgctacgaa
     7561 acgctgggcg gaaagggcgt tgcacccctg cgcgtcgggc cgcggccggc tcctcctgca
     7621 gcgagcccgg ggggcgaggc gtgaaggcgc ctggaccgaa a
//
LOCUS       DASVDU010000210        29029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1205181, whole genome shotgun sequence.
ACCESSION   DASVDU010000210 DASVDU010000000
VERSION     DASVDU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 29029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29029
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205181"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1107
                     /gene="pqqB"
                     /locus_tag="VF226_13425"
     CDS             76..1107
                     /gene="pqqB"
                     /locus_tag="VF226_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEX7075036.1"
                     /translation="MTTAIHLTPQQRGGTLTKFNRAVRSRPVLPHAHASAMIIKVLGS
                     AAGGGFPQWNCNCRNCADVRRGVAGLEPRTQSSLAVSVDGRNWTLINASPDLRQQISD
                     HPELSPGYGDPLRSSPIRSVVLTNADIDHIAGLLNLREGQPLTIYADPRVLSALAGSP
                     VFGVLDARYVERAPIVNDAPFEPAGCPGLSIEGFPVPGKVALYLEDESAWPDFGTAPG
                     DTIGLKLVDRHTGRFACYVPGCAAVDEPLLRRMSGAAAILFDGTLFTDDEMIVQGLSQ
                     KTGRRMGHISMSGPDGSVAALAGLNVGRRIFTHINNSNPALRRGSPERAWIEAAGWEI
                     AHDGMEVLL"
     gene            1107..1841
                     /gene="pqqC"
                     /locus_tag="VF226_13430"
     CDS             1107..1841
                     /gene="pqqC"
                     /locus_tag="VF226_13430"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX7075037.1"
                     /translation="MSPMTPEELERQIRAVGAERYHDKHPFHRLLHSGKLDKGQVQAW
                     ALNRYCYQAAVPRKDAALMSRTTDRELRREWVHRILDHDGSGEEEGGIERWLVLTDGL
                     GLDRDYVTSMAGALPATRFAVEAYVNFVRERSLVEAVASSLTELFAPSIHRERIAGML
                     EHYDFIDEKVMAYFRRRLSQATRDADFALDYVKRHATTRELQELCIDAVRFKCNVLWA
                     QLDALHHAYVTPGLIPPGAFRPGPAA"
     gene            complement(2124..2501)
                     /locus_tag="VF226_13435"
     CDS             complement(2124..2501)
                     /locus_tag="VF226_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075038.1"
                     /translation="MLPTLHAKSPKVPQLELFQKLEQYASIRLGNLTTGHRRVNDRGV
                     QQQKETAKIALKRDTEVRTISAVPQQVCAMDAGNEKAAPVGRLLQEMVLPVRIERTTS
                     PFIAPALSRPPLAAFVRWTIPSS"
     gene            2500..2958
                     /locus_tag="VF226_13440"
     CDS             2500..2958
                     /locus_tag="VF226_13440"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7075039.1"
                     /translation="MDEVAAREAFETALRTQRPDFETFFLSRLFGLSFTYTDETCRID
                     FPIRDFMFNPQGTLHGGVAALVLDVAMGHLLKRSLGVPGTTLEMKVQYLSPLRPPGAS
                     CESRFLRRGQRIAFLEARMWDAEGTLAAVANATWRVNRPAPGAPKKETEA"
     gene            3023..3910
                     /locus_tag="VF226_13445"
     CDS             3023..3910
                     /locus_tag="VF226_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX7075040.1"
                     /translation="MKPTILTCAVTGTFPTREHNPNLPVTPEEIANACIAAAKAGAAI
                     CHIHVRDPETGRPSMKLDYYREVVQRLRASDVDLIINLTTGPGGRFIPSEDEPARAAP
                     GTMLTTPEIRTEHIVELKPELCSLDLNTMWFGGGAVINSPRNIRIMAERIYAAGVKPE
                     LEVFDTGDILLSRDLFTEGTLRAPALFQIVTGVKYGMAATPESMCYAKSLLPRDCEWA
                     AFGAGRMAFPMLAQAFVLGDHCRIGMEDTVHLAKGVLAPDNAALVEKAVRIIESLGGK
                     VASTAEARQILGLADPRAG"
     gene            complement(3920..4183)
                     /locus_tag="VF226_13450"
     CDS             complement(3920..4183)
                     /locus_tag="VF226_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7075041.1"
                     /translation="MSIIAWIIVGLIAGWLAEQITGRDQGLLTNLLVGIVGAFVGGFL
                     FSTLLGFEYRQGFNIASIIVATVGAIVFLFILSWIRGESRQIR"
     gene            complement(4326..4904)
                     /locus_tag="VF226_13455"
     CDS             complement(4326..4904)
                     /locus_tag="VF226_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075042.1"
                     /translation="MPSPVRSIVPLVTAAVASGVLSYATEGLPRLSHELQALLTSYLI
                     NPQLMLPGVWFGLVTGALAWRLGSRGAIGAVLSLVITWVGWQLAVQAGIAAFDRLGGM
                     TTDETIRLAAAGVGGGAVGALVSFIGVRIGTPMRRTLLAGFATLAVGAAFGLLLVWSS
                     TRQSAGLLLYATWQPAVIAVMAYFANSRSAQA"
     gene            5089..5790
                     /locus_tag="VF226_13460"
     CDS             5089..5790
                     /locus_tag="VF226_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7075043.1"
                     /translation="MPTPDIPKVEAAIVEMTNAIRKQHNLNVVKPNPDLAEAAQGYAR
                     FLATSAKFSHTADGRHPSDRAAAAGYQACSVAENLSSNLDTRGFETRQLAREAVQGWL
                     NSPGHRRNLLAEHVTHIGVAVAKAPGEERYISVQMFGRPQELAYRFRVNNASGQTVSY
                     TFLGKAYDIKPRYSSRHTSCAPGEIAFRKPGSEKVVARYKARDGDTFVLRRTDDGLKV
                     ELVRSEEKPGKQLSN"
     gene            complement(5855..6589)
                     /gene="recO"
                     /locus_tag="VF226_13465"
     CDS             complement(5855..6589)
                     /gene="recO"
                     /locus_tag="VF226_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX7075044.1"
                     /translation="MNWSDEGIVLSVRPHGETAAVLELLTRAHGRHLGLVHGGRSRRL
                     RPVLQIGNHVEATWKARLADQLGFLTVELRKGYAASAMEDALALAGLSSLCALARLLP
                     ERDPHPDLYEITLFVLGYLDDNGVWPALYVRWELNLLAELGFGLDLSSCAATGSTENL
                     VWVSPKSGRAVSSAAGEPYRHRLLPLPPFLTGDHRGPVTPRDVLDGLKLTGSFLEARV
                     LSPRDMAMPEARHRLVSALSRAAERS"
     gene            complement(6623..7561)
                     /gene="era"
                     /locus_tag="VF226_13470"
     CDS             complement(6623..7561)
                     /gene="era"
                     /locus_tag="VF226_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX7075045.1"
                     /translation="MAEPEATRAQVPESDTRCGFIAVIGAPNAGKSTLVNALVGAKVT
                     IVSRKVQTTRIPVRGIVVDGQSQIVFLDTPGIFRPRRRLDRAMVEAAWAGALDADIVV
                     LLVDAVKGVDEDVERILQRLGDAAGIKVLALNKVDRLAEKHRLLELTADLTKRVPFER
                     VFMVSALTGSGLKDLKDYLVSAVPPGPWHYPPDELSDLTDRKLAAEITREKIYERLHE
                     ELPYSVTVETTDWQTRRDGSARVEQTIFVERESQRKIVLGKGGATIKQISMEARKELA
                     KLLGHEVHLFLHVKVRENWADDPERYREMGLEFPKE"
     gene            complement(7554..8294)
                     /gene="rnc"
                     /locus_tag="VF226_13475"
     CDS             complement(7554..8294)
                     /gene="rnc"
                     /locus_tag="VF226_13475"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX7075046.1"
                     /translation="MRRHKKSTDLEKHLGYTFKNRELFERALTHASVRSQRGARVDNE
                     RLEFIGDRVLGLAIAELLHENHPDATEGELARRYNRLVRGQACAAVARKIGVGPHLIL
                     SDSEAESGGREKDTILADALEALLGAIFIEAGFETARKVVRALWTTQFEALPHVVADP
                     KSALQEWAQGRGLDLPEYIEISRSGPDHAPRFVTEVHIAGCKPARGEGATKRQAEQAA
                     ASSLLAREGVRSDIGRHAGNSLKKPSHG"
     gene            complement(8303..9073)
                     /gene="lepB"
                     /locus_tag="VF226_13480"
     CDS             complement(8303..9073)
                     /gene="lepB"
                     /locus_tag="VF226_13480"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX7075047.1"
                     /translation="MSLNTDTSKKRESGWWETVKIVIQALALAMVVRVFFFQPFNIPS
                     GSMKPTLLVGDYLFVSKLSYGYSRYSFPFGINLFPGRIFASKPQRGDVAVFKLPRDNS
                     TDYIKRVIGLPGDEIQMRGGVLYINGQAVPKKRIGDFVTREEDGVLRRISQYEETLPN
                     GVTYTVLDSDPNGPFDNTGVFKVPENHYFMMGDNRDNSTDSRAQWGVGFVPFENFVGR
                     AEIIFFSGAFDDPNAFRLYTPWQWPWDIRWDRFFNLVR"
     gene            complement(9196..9609)
                     /gene="acpS"
                     /locus_tag="VF226_13485"
     CDS             complement(9196..9609)
                     /gene="acpS"
                     /locus_tag="VF226_13485"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7075048.1"
                     /translation="MILGLGNDIVDIERIERTIARFGDRFLERIFTQTERRKSDRRKE
                     RAASYAKRFAAKEACSKALGTGFRQGVFWRDMGVINLPSGRPTLMLTGGAAEQLKRIT
                     PPGYTARIDLSLTDDYPLAQAIVIISGVPLDTSSP"
     gene            complement(9616..10398)
                     /locus_tag="VF226_13490"
     CDS             complement(9616..10398)
                     /locus_tag="VF226_13490"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEX7075049.1"
                     /translation="MSSHLDIPFQRLGVNIDHVATIRNARGGRHPDPLRAAHLAVEAG
                     ADGITAHLREDRRHISDNDIARLKSELTRPLNLEMAATEEMLAIALRHLPNACCLVPE
                     RREERTTEGGLDVAGGREHLKGYVARLKEAGIRVSLFIEPDERQIEAAAAIGSDIVEL
                     HTGAYCERALEENARELAKEMERLRVAARIAADAGLEVHAGHGLTYNTVSPVARIPQV
                     VELNIGHFLIGEAIFGGLHSAVRRMRALMDEARASVTGRRSA"
     gene            complement(10502..12733)
                     /locus_tag="VF226_13495"
     CDS             complement(10502..12733)
                     /locus_tag="VF226_13495"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX7075050.1"
                     /translation="MMRQYELVERVLDYDPKADEALLNRAYVYAMKAHSNQKRASGDP
                     YFSHPLEVAAILTELKLDDATIATALLHDVIEDTEATRAEIDQLFGPEIGALVDGLTK
                     IRRLDLVSKRAEQAENFRKLLIAISSDIRVLLVKLADRLHNMRTLGYMRPETRTRISQ
                     ETLDIYAPLAGRMGMQQLREELEDHAFRWLNPDAYQTVTARLAQLRERNRGIVDEIQT
                     ALRNKLAEENIDAEVSGREKKPYAIWRKMQNKQISLEQLSDIYGFRVIVRSIPDCYRV
                     LGVVHTTWSSVPGRFKDYISTPKQNGYRSIHTTIVGPRHQRVELQLRTREMHHVAEYG
                     VAAHALYKDNGSNGSRATYEKDSGPYMWLRRLVETLLDGNNPEEFLEHTKLELFHDQV
                     FCFTPKGRLIALPRGATPIDFAYAVHTDIGNSTVGALINGRQMPLSTMLRNGDEVRIL
                     TASGQTPPAAWERLAVTGRARSAIRRASRDALRRQYSELGRRLLAARFDKAGVEYSDE
                     KLKAVLHRLGQKTIEDTLAAVGRSELPVADVLRAVAPDVADPEHRPKSYRPKNRHSHA
                     TSQEGWFNLSKVIGLKFRFTGNSSDTSSRPGGLPIRGLRNDLPVSFEEGGAVPGDRVV
                     GVMMPGEGIRIFQIHSPRLKDFEKESWIDVTWDISPENPERFPARLKVTAINEPGSLA
                     QIAQVIGEADGNIDNLRMTHRASDFTEMLLEVEVFDLEHLNRIIAGLRAKPVVSIVER
                     SFD"
     gene            complement(12989..13417)
                     /gene="rpoZ"
                     /locus_tag="VF226_13500"
     CDS             complement(12989..13417)
                     /gene="rpoZ"
                     /locus_tag="VF226_13500"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEX7075051.1"
                     /translation="MARVTVEDCVDKVPNRFELVLLAAHRARAIANGSPITVEPENDK
                     NPVIALREIAEQTIPPDDMRESLIHSIQKNVEVDEPEAAGAPVLAPERRSPLLGRDDQ
                     ASDNVVDVLTEEQLLRGMESLTPTEPSAAIGGNGGGRNGR"
     gene            complement(13495..14037)
                     /gene="folK"
                     /locus_tag="VF226_13505"
     CDS             complement(13495..14037)
                     /gene="folK"
                     /locus_tag="VF226_13505"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX7075052.1"
                     /translation="MKSVLISIGANLAGAWGAPIRTLRRAIKELQNRRITVEAVSPLY
                     ETEPIGGNWQPPYLNAVILAKAKVAPATLLLRLKSLERRAGRRLGVHWGPRPLDLDIV
                     DFGSSKYGWPPGTRRRGRIILPHPEAHRRAFVLVPLLDVAPKWRHPVTGIPGRTLLAR
                     LGPQRRGVRRILDSQWFLCK"
     gene            14255..14899
                     /locus_tag="VF226_13510"
     CDS             14255..14899
                     /locus_tag="VF226_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7075053.1"
                     /translation="MHFHPDEKIALFIDGANLYATAKALGFDIDYKRLLALFRKQGRL
                     IRALYYTALAEDQEYSSIRPLIDWLDYNGYTMVTKPTKEFTDSMGRRKVKGNMDIELT
                     VDAMRLSRTVDHIVLFSGDGDFKSLVGALQQEGRRVSVVSTLQTQPPMVADELRRQAD
                     QFIDLADLEQFICRDPATRPIRNDRAYSGRREGYQRPATEPVPFQDQDDLSEEV"
     gene            14915..15604
                     /locus_tag="VF226_13515"
     CDS             14915..15604
                     /locus_tag="VF226_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7075054.1"
                     /translation="MHGLSDSAASELSPDAAEPSRDCPLCPRLVAYRLANRAAEPGWY
                     NAPVHSFGDPSARLLIVGLAPGLRGANRTGRPFTGDFAGNLLYETLLEFGFAAGHYRA
                     DPNDGLQLIDCMITNAVRCAPPQNKPLPSEINTCRRFLRARIGELQRLRVIMALGRIA
                     HESVLAALDVRKSAFPFGHGAQHRVRPGLVLFDSYHCSRYNTSTGKLTPQMFKEVIRT
                     IGNCLGDLADH"
     gene            15779..16180
                     /locus_tag="VF226_13520"
     CDS             15779..16180
                     /locus_tag="VF226_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEX7075055.1"
                     /translation="MLEIAPEKVAHIIIKAREYDSKVDVHIPDEGSGSEGEPDDVLES
                     MPDDTTRPELVSFIDSLNEDEQAHLVALAWVGRGTFEPEEFDEAVATARQEKIVSTSE
                     YLLGMPLLADYLAEGLEKLGIPVEDIESEIL"
     gene            complement(16270..16776)
                     /locus_tag="VF226_13525"
     CDS             complement(16270..16776)
                     /locus_tag="VF226_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7075056.1"
                     /translation="MRLTRQTSHAIRILIHGARAPDKLIKVADIAGELDITPQNVFKI
                     VHLLARAGFVQAVRGRNGGVRLARDPREIRIGEVVRAMEVMDIALDGEVPRKGKAGSP
                     PINRMLDDALEAFISVLDQHTLADMAGTGRAPGYLSEPKARSPQRNRRRVAAARKNLT
                     RESASPSR"
     gene            complement(16881..17387)
                     /gene="hutX"
                     /locus_tag="VF226_13530"
     CDS             complement(16881..17387)
                     /gene="hutX"
                     /locus_tag="VF226_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007754953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HEX7075057.1"
                     /translation="MSKHRLLAEKLAANPDGVLERIAAECGVSTLDVIKALPENCRTV
                     VPGGLFTEIMADLETWGEVLVIVHTPDIVLECAGRIPPGSVGRGYFNLHGDSPIGGHI
                     RHENCAEIAFVSRPFNGRPSCSIQFFNGEGGAMFKVFVRRDAERNLLPDQIARFEQLR
                     LRCAAVPN"
     gene            complement(17414..17755)
                     /locus_tag="VF226_13535"
     CDS             complement(17414..17755)
                     /locus_tag="VF226_13535"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7075058.1"
                     /translation="MYIAMNRFKVAKGSEELFEKLWLSRESYLHELPGFITFHLLKGP
                     EREDHTLYASHTVWRSIADFENWTKSEQFRSSHARAGSSAIRPLYFGHPEFEGFHVLQ
                     EIKAPAEQAAA"
     gene            complement(17850..19931)
                     /locus_tag="VF226_13540"
     CDS             complement(17850..19931)
                     /locus_tag="VF226_13540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEX7075059.1"
                     /translation="MFGRRGRSTAKLWALHLSVSASALAIASASASAQTVQLDGIVLT
                     FSKMSESAIEALSGSSAIDNEQLDQQFQADSVSQIMDTIPGVTTQENGLDTAQAINIR
                     GLQDFGRVNVLIEGARQNFQKSGHNADGTFYLEPEMIKRVDVTRGPTSTIYGSGAIGG
                     VAAFELLDADDILLPGEYAAVRTRTRYGTNASSKLGSVTGAVRVGNFDVLGQLNGRWS
                     DDYEDGGGNTINNSGDTTKSGLVKARWELAPGHQLTGTFIDYRSDFVDQTDRGAEFDT
                     TVDNKQYTLGYTFLSPDNPLLDFSAKVYRNDTRLDQTRLVTNPYDRSFDVETTGFDIF
                     NTSRFQIGSAKVALTYGGDGFEDRVTTFAEQGDSAVALTPGGRRTAAGAFIQSKATFF
                     DTIDIIAALRYDQYEIESDDYSSEGSRVSPKITAGVTPFHGVTFFAGYAEGYRAPSIT
                     ETLMDGVHPSPAFDILPNPFLRPEVAHNLEAGINLKWDELLRPRDAFRAKLVAFRNKV
                     DDYIDMVDRKDLPFGCAIPGWPPPPLPPQTPCPEAARLYAQQYQNIANATIEGIEFEA
                     MYDARSWFVGIGAHRIRGEDDDTGDPLNTVPADQITVTLGMRAFDEKLIAGTRTRFVA
                     GQKRVTTASLATDGYTVVDLFGEYAVNENTTVNVNIDNLFDREYRQFRDLENSPGFTA
                     RVGLTMRLGAQ"
     gene            20192..20377
                     /locus_tag="VF226_13545"
     CDS             20192..20377
                     /locus_tag="VF226_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEX7075060.1"
                     /translation="MGGTSPIKNRKAPSANDTQAPKAVRMRVSDLLADTREAILEHNG
                     QEYRLRITANGKLILTK"
     gene            20390..21301
                     /locus_tag="VF226_13550"
     CDS             20390..21301
                     /locus_tag="VF226_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075061.1"
                     /translation="MSRTIPGFVTLAIVAAVLGATASIAAEVVDARGEVIKADNPSRI
                     LSIGGDTTEIVYALGFGSNVVAVDTSSQFPPEVLAKKKVGYMRALSTEGVLSTGATLI
                     LANAHAGPPEVIRALATSSVPLVMLPENASPESLIEKVKLVGRALGAEAKANDLAAQL
                     NGQFGSLETVRSKIERPVRALLVLSVDGGRALAAGRGTTADLMLELAGATNAAADLNG
                     YKPVSDEALIEMAPQAIVAVRRAPEEEIAADIAALPGFKALGAGRKVPIITMDAAYLL
                     GFGPRAPRAAGELMAQLYPDLPNQVAR"
     gene            21298..22392
                     /locus_tag="VF226_13555"
     CDS             21298..22392
                     /locus_tag="VF226_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7075062.1"
                     /translation="MTIESLDLPAVEDGIDRHGLGRRLILAGLLALGLAAVASVSVGP
                     TGLSLFSLPRAISAALSGSDAAADVHARLVLLEVRLPRTILAAFVGASLAVAGAMMQG
                     LFRNPLADPGLIGVSSGAALAAVATIALGGGFAGPWVRALGIWALPVSAFLGGFLTTM
                     VLMLIARKHGTTAIATLLLAGIALAAIAGALTGLIAYVTDDRELRDLTLWSMGSLAGA
                     SWQKVMAIAPFALILFVTIPRFVRALNGMLLGEAEAFYLGIEVERAKFWIVVLTAAAV
                     GAAVAVAGTIGFVGIVVPHLVRLTAGPDHRIVLPGSALLGAVLVVVADIAARMIVAPA
                     ELPIGIVMAVIGAPVFLHLVIRRGGGLAAP"
     gene            22389..23192
                     /locus_tag="VF226_13560"
     CDS             22389..23192
                     /locus_tag="VF226_13560"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HEX7075063.1"
                     /translation="MIRAEAVTYAVGNRSLLSRVDLDLQAGRLIAVIGPNGAGKSTLL
                     RLLTGELKPTGGRILIDGQDLATLSADQLARRRAVVSQHTALAFPFTALEVVLLGILV
                     PGFEADCSGTRRIARDMLARLGMEHLADRTYTTLSGGERQRVHMARALCQLACGRVPA
                     EKTAILVDEPTSSLDIGHQLLVLDELTRQKNAGRAVLAVLHDLNLASTYADEMLLLAD
                     GAVLARGSPAEVMTDERLSMAFGCDVRTNCVPRNGAPYLLPQSCTAVAT"
     gene            complement(23227..24342)
                     /locus_tag="VF226_13565"
     CDS             complement(23227..24342)
                     /locus_tag="VF226_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HEX7075064.1"
                     /translation="MAIDRRAFLAGAGGLLASGLAAQGAPAETRAADEPLFAAARRSP
                     DGAFSAAIFRPDGTDVNAVALPARGHDVTVCPVTRTCVAFARRPGNFAVAFRRDKGWK
                     PIRFTTPPDRHFYGHGIFSRDGRILFATENDFEAGRGCIGLYDATAGFRRIGEMESHG
                     VGPHDIALMKDGRTLVIANGGIETHPAIGGGRTQLNRATMSPSLAYVDTITGDLVERH
                     ELPEGLRQLSLRHLDVGAGGMVVIGGQFKGDVTAGTPLILRHRLGGLPKTVELAADLS
                     PRLRGYISSVAVDASGEIAALTGSRGGIVLFIDVATGRVLGERSLVDVSGVAPSVGGF
                     LLTTGKGEIASGASPSQGATMTSSTHWQWDNHAVLVS"
     gene            complement(24332..25438)
                     /locus_tag="VF226_13570"
     CDS             complement(24332..25438)
                     /locus_tag="VF226_13570"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX7075065.1"
                     /translation="MPTFRSKLTALALVAFIGAGPAASAEQRAATDFDHALVARTALE
                     KHIRPGFRRLTSAFAELQQATGSCEKQPQFRYARLRPVFRKAVLAWGRVAHLNFGPLA
                     AENRYDRVFFWLDRKGIARRQVARALKESPSEYRDAAKLAQSSIGVQGLPALEQLIAT
                     PAEPGENSSFRCSYAQAIAGNLVNIAREVEAAWSEGGIWSERWLTPGPGNPTYIQPSE
                     TTYALIRAYLDNIERVRDVELMRPLGLAVRGRDLPGPFARSDLTMAFIGARITGLRSL
                     IEDGGLAKETTRTAKARGSPAAEQAMKQVRFELRLLTRLSTPLTKVPDFFGSSQRDEA
                     IALGFPLKSARFQAERAFSLTTDLPMGFNASDGD"
     gene            complement(25452..26975)
                     /locus_tag="VF226_13575"
     CDS             complement(25452..26975)
                     /locus_tag="VF226_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX7075066.1"
                     /translation="MFSREKQKEQRRLRSAQGFLAAAASLPFLLVGAGLSWSENSTGA
                     EAFPAAFDPAEGHPGGEATSLGSTDNTNAFSHSSANMGFARELDFKIGNAIFRKLWVS
                     APASTKSSDGLGPLYNARSCQTCHLKDGRGHPPNGNWPDDNAVSMLLRLSIPPQTDAD
                     RELLASGRASVIPEPTYGTQLQDLAIAGHKGEGHVHISYEELPVTLADGEVVHLRKPT
                     YTITDLGYGPMHPETMVSPRVAPPMIGLGLLEAIPEADILAGEDPDDRDGDGISGRAN
                     RVWSIEHNKLMLGRFGWKAGAPTVLQQTAEAAAGDMGLGSVLVPKPSGDCTERQPTCL
                     AAPHGGTAKAPGEEFKTQLLKLTAFYARNLAVPPRRNPDAPDVLAGKALFHSIGCAAC
                     HRPSFMTGTEASDAHLAGQHIWPYTDLLLHDMGEGLADGRPEGRADGREWRTAPLWGI
                     GLTETVSGHTYFLHDGRARNLAEAILWHGGEAQAARDAFAALDPADRARLIAFLNSL"
     gene            complement(27017..28288)
                     /locus_tag="VF226_13580"
     CDS             complement(27017..28288)
                     /locus_tag="VF226_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HEX7075067.1"
                     /translation="MRRLVDWFLTLGALSVMATSAMAAAPAPRDVLDTYADIALAGYE
                     DSLTTAKALQAKIEAFLAEPTEERLAEAKAAWLAARVPYQQTEVFRFGNRIVDDWEGK
                     VNAWPLDEGLIDYVDGDYGTESEENPLYVANIIANPSVRISGRMVDASKITKELLSDT
                     LHEAGGVEANVATGYHAIEFLLWGQDLNGTDAGAGARPASDYDTANCTGGNCERRAQY
                     LRTVTELLVDDLTWMVEQWKADGAARKALTADETQGLVAIFTGLGSLSYGELAGERMK
                     LGLLLNDPEEEHDCFSDNTHNSHYYDLIGIRNVYFGKYERIDGSVISGPSVSDLVKAK
                     DAEIDARVRDALDESLAQFETLKKRAETVEAYDQMIGEANDEGNAVVEAALQALIAQT
                     REFERAIAALELGAVQFEGSDSLDNPSKVFQ"
     gene            complement(28740..>29029)
                     /locus_tag="VF226_13585"
     CDS             complement(28740..>29029)
                     /locus_tag="VF226_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6522 family protein"
                     /protein_id="HEX7075068.1"
                     /translation="MQMSLERTDDGFCIDATLLGELLGVPPRGVQALMRNNEITSACE
                     RGEGEHEGQYRLTFFYKNRRARLAVDASGRILKRSVVDFGEHPIPGRAANR"
BASE COUNT         5247 a         9272 c         9097 g         5413 t
ORIGIN      
        1 cctcgacggc tgcgagtgtc gaccaacagg tagctgcatc gagctctgcc agcaagtgtc
       61 cgagtcaacc ggaaggtgac gaccgcgatc cacttgacgc cccaacagcg cggcggcacg
      121 ctgacgaagt tcaaccgggc cgttcgtagt cggcccgtgc tgccccacgc ccacgcgagc
      181 gcaatgatca tcaaggtcct gggttcggct gccggcggcg gtttcccgca gtggaactgc
      241 aattgccgta actgcgccga cgtgcgccgc ggcgtcgccg gtctggagcc gcgcacgcag
      301 tcgtccctgg cggtgagcgt cgacgggcgg aactggacgt tgatcaacgc ctcgccggac
      361 ctgcgccaac agatttccga ccacccggag ctcagtcccg gctatggcga tccgctccgc
      421 agcagcccga tcagaagcgt ggtgctgacc aatgccgata tcgaccatat cgccggcctc
      481 ctgaacctga gagaggggca gccgctgacg atttatgccg acccgcgggt cctgtcggca
      541 ctggcgggaa gtcctgtttt tggggtcctt gacgccagat atgtcgaacg agctcctatt
      601 gtgaacgatg caccgttcga gccggccggc tgccctggcc tcagcatcga aggcttcccc
      661 gtgccgggga aggtcgccct ctacctggaa gacgagtcgg cgtggccgga tttcggcact
      721 gctcccggcg acacgatcgg cctgaaactt gtcgatcgcc acacaggtcg tttcgcctgc
      781 tatgtgccgg gctgtgcggc cgtggatgag ccgctgcttc gccgcatgtc cggagcggcc
      841 gccatccttt tcgatggcac gcttttcaca gacgacgaga tgatcgtgca aggcctgtcg
      901 cagaagaccg gacgccgcat ggggcatatc agcatgtcgg ggccagatgg ctcagtggcg
      961 gcccttgccg ggctcaacgt cggacgccgc atcttcacgc acatcaacaa ttcgaatccg
     1021 gccctgaggc gaggctctcc cgagcgagcc tggatcgaag cagccggttg ggaaatcgcc
     1081 cacgacggca tggaggttct cctgtgatga gcccgatgac gcccgaagag ctcgagcggc
     1141 agatccgcgc cgtcggcgcc gagcgctacc acgacaagca cccgttccac cgcctgcttc
     1201 actctggcaa gctcgacaag ggccaggtgc aggcatgggc gctcaaccgg tactgctacc
     1261 aagccgcggt gccgcggaag gatgccgccc tgatgagccg cacgaccgat cgcgagctgc
     1321 gccgcgagtg ggttcaccgc attctcgacc atgacggcag cggtgaggag gaaggcggga
     1381 tcgagcggtg gctcgtcctg acggatgggc tcggcctcga ccgtgactac gtaacatcca
     1441 tggccggcgc cctgcctgct acgcgatttg ccgtcgaggc ttacgtaaat ttcgtgcgtg
     1501 agcgctcgct ggtggaggcc gttgcctcct ctctgacgga gctgttcgcg ccctcgatcc
     1561 atcgcgagcg gatcgcaggg atgctggagc actacgactt catcgacgag aaggtgatgg
     1621 cctacttccg ccggcgtctc agccaggcga cccgcgacgc agattttgcc ctcgactatg
     1681 tcaaacggca cgccacgacg cgtgagttgc aggagctttg tatcgacgcg gtccggttca
     1741 agtgcaacgt gctctgggca cagctcgatg cgcttcacca cgcctacgtc acgccgggcc
     1801 tcattccgcc gggcgccttc aggccaggac cagcggcctg agcttggtgc cgtaggcccg
     1861 agccgtttca ttcgtcgcct tgcgcactca acggcaatcg gttagaccgg gcgcgaccag
     1921 gagacatgct gccgtagctc agtggtagag cactcccttg gtaaatttgc ccgcttggcc
     1981 ggaaacggcc aggagcgcac cgcccaaatt cggggaaacc gtccggtccg gccgacggca
     2041 atcccgagcc aagcccggag acccgggaag gtgtagaggc cagacgggtg gcatctaagg
     2101 gtccctctct cagaaggcgg gacctatgat gaagggatgg tccagcgcac gaacgccgcc
     2161 agcggcgggc gcgaaagcgc aggtgcgatg aagggagagg tcgtgcgttc aatccgcacc
     2221 ggcagcacca tttcttgcag aagccgccca accggagcgg ctttttcgtt tccggcatcc
     2281 atggcacaga cctgctgcgg gacggcgctg atggtacgga cctcggtatc tcgcttcagc
     2341 gctatttttg cggtttcctt ttgctgctgg acccccctat cgttgacccg acgatgccct
     2401 gtcgttaggt tgcccagccg aattgaggcg tactgttcca gtttttggaa cagctctaat
     2461 tggggcactt tgggcgattt tgcgtgaagc gttggaagca tggatgaagt tgcggcacgt
     2521 gaggccttcg aaacagcact cagaacgcag cgccccgatt tcgagacgtt cttcctgtcc
     2581 cgtctgtttg gcctttcctt cacgtacacg gacgagacct gccgcatcga cttcccgatc
     2641 cgcgatttca tgttcaatcc gcaaggcacc ctgcacggcg gggtcgccgc gctggtcctc
     2701 gacgttgcga tgggccacct tctgaaacga tcgcttggcg tgcccggcac gaccctcgag
     2761 atgaaggtcc agtacctctc ccccctgcgc ccgcctggcg ccagttgcga atcgcgcttt
     2821 ttgcgaagag ggcaacggat cgcattcctc gaggcgcgca tgtgggatgc ggaggggacg
     2881 ctcgcagcag ttgccaacgc gacgtggcga gtcaacaggc ctgctccagg agctcccaaa
     2941 aaagagacgg aggcctgagc gagagggcgt gacacctgcc gaagccccgc cggaccggac
     3001 gcgacttgct ggaggacaga cgatgaagcc gacgatcctg acctgcgccg tgaccggcac
     3061 ctttcccacg cgcgagcaca acccgaacct gccggtcacg cccgaggaga tcgccaacgc
     3121 ctgcatcgca gcggcaaagg ctggcgccgc catctgccac attcatgtcc gcgaccccga
     3181 aacggggcgt ccaagcatga agctcgatta ctacagggag gtcgtccagc ggctgcgcgc
     3241 gagcgatgtc gacctcatca tcaacctgac caccggtcct ggtggccgct tcatcccgag
     3301 cgaggatgag ccggccaggg cggcgccggg gacgatgctc acgacgcccg agatccgcac
     3361 cgagcacatc gtcgagctca agccagagct ctgcagcctc gacctcaata cgatgtggtt
     3421 cggtggcggc gcagtgatca actcaccgcg caacatccgc atcatggccg agcggatcta
     3481 tgcggcaggc gtgaagccgg aactcgaggt cttcgatacc ggcgacatcc tgttgtcgcg
     3541 ggacttgttc acggagggca ccctgcgagc gccggcgctg ttccagatcg tcaccggcgt
     3601 gaagtacggc atggccgcca cgcccgagag catgtgctat gcgaaatcgc tgttgcccag
     3661 ggactgcgaa tgggccgctt tcggggctgg ccggatggcg ttcccgatgc tcgcgcaggc
     3721 ttttgtgctg ggtgaccatt gccggatcgg catggaggat accgtacacc tcgccaaggg
     3781 ggtattggcg cccgacaacg ccgcgctcgt cgagaaggcg gtgcggatca tcgaaagcct
     3841 cggcggcaag gtcgccagca cggcggaggc ccgccagatc ctcgggctgg cagatccccg
     3901 cgccggctaa gctggcgctt tagcgaattt gccgtgattc gcctctgatc cagctcagaa
     3961 tgaagaggaa aacgatcgcg ccgaccgttg caacaatgat cgaggcgatg ttgaagccct
     4021 gcctgtactc gaagccgagc agtgtgctga acaaaaaccc gccgacaaag gcgccgacga
     4081 tgccgacaag caggtttgtc agcaatcctt ggtctctacc cgtaatctgc tccgcaagcc
     4141 agccggcaat caggccgacg ataatccagg cgatgatact caccgcagtc tctcctgttg
     4201 gggccacgta cagcacttaa cgcctgacct gctcgagagt tcactagccg ttccacctgg
     4261 tgaacatcgg acggcgctgc agccgtgtac gtccaaagga gagccccctt gtgctttatg
     4321 ctccttcatg cttgcgcgga cctgctattc gcgaagtaag ccatgacggc gatgaccgcc
     4381 ggctgccaag ttgcatagag cagcaatcct gcgctctgcc gggtggatga ccagaccagc
     4441 agcaggccga acgcagctcc caccgccaag gtcgcaaagc cagcaagcag cgtccgtcgc
     4501 atcggcgtgc cgatccgcac gccaataaag gacacgagcg ccccgacggc gcctccgccg
     4561 acgccggcag cggcgagccg gatggtctcg tcggtggtca tgcctcccag cctgtcgaac
     4621 gcggctatgc cggcctgcac cgccagctgc cagccgaccc acgtgatgac gagcgacagc
     4681 acggctccga tcgcgccacg agatccgaga cgccaagcca aggcaccggt gacgagcccg
     4741 aaccagactc caggcagcat gagctgcggg ttgatgagat aagacgtcag aagcgcctga
     4801 agctcatgag agagccgcgg cagaccctcg gtggcatagc tgagtacgcc agatgcgacc
     4861 gcagccgtga ccagcggaac gatgctgcga accggtgatg gcatggcccc ctcgccaatg
     4921 acccgctgcc gccttttcgc gacgatctga ggcgaagttg tggtttcgga aacgaggatg
     4981 tcgaacgaca agtaaaccgt cgcgccgctt cgcttctcgc cacctgacgt tgctccttcc
     5041 gcaaatgtgc tatacggacg ggttcccttc ggagcgggat attcggacat gccgacgccg
     5101 gacattccaa aagtcgaagc tgcgatcgtc gagatgacga acgccatccg gaagcagcac
     5161 aatctcaacg tagtcaagcc gaatcccgac ctggcggaag cggcccaagg gtacgcgagg
     5221 tttctggcga cctccgcaaa gttctcccat accgcggatg ggaggcaccc atccgatcgc
     5281 gccgccgccg caggctacca ggcctgctcg gtggccgaga acttgtcctc caatctcgac
     5341 acccgtggct ttgagacccg gcaacttgct cgcgaggcgg tgcagggctg gctcaattcg
     5401 cccggacacc ggagaaacct gctggccgag catgtcactc atatcggagt tgccgtcgcc
     5461 aaggctcccg gcgaggagcg gtatatttct gtccagatgt tcgggcgacc gcaggagctt
     5521 gcctatcgct ttcgggtgaa caacgcctcg gggcagaccg tcagctacac attcctcggc
     5581 aaggcatacg atatcaagcc gcgctattca tcccggcaca cgtcctgcgc tcctggcgag
     5641 attgcgtttc gaaagcctgg ctcggaaaag gtcgtcgcgc gctacaaggc tcgtgacggc
     5701 gatacgttcg tgctccgccg gacagatgac ggcctgaaag tcgagctggt gagaagcgag
     5761 gaaaagccag gcaagcagct gtccaactga acgcgcggct atcggctccg ggcggcgggc
     5821 cggccactcc tgtctccagt tggaatggct tgcctcagct ccgctcggcg gcgcgggaca
     5881 acgcagagac cagcctgtgt cttgcctctg gcatggccat gtcgcgcggg ctgagcacgc
     5941 gggcttccag gaatgagccg gtcagtttca ggccgtcaag cacatccctg ggcgtcaccg
     6001 gcccgcgatg gtcaccggtg agaaagggag gcagcggcag cagacggtgc cggtagggct
     6061 cgccggcggc agacgaaacg gcacggccgg acttgggcga gacccaaaca agattttcgg
     6121 tgctgcccgt tgcagcgcag gatgagagat cgaggccgaa gccgagctcc gccagcagat
     6181 tgagctccca cctgacgtac agcgccggcc agacgccatt gtcgtcgaga tagccgagga
     6241 cgaacagcgt gatctcgtag aggtccgggt gcggatcgcg ctcgggcagg agccgcgcca
     6301 gcgcgcagag cgaggagagc cccgcgagcg ccagcgcatc ctccatggcg gaagccgcat
     6361 agcccttgcg cagctctact gtgaggaagc cgagctgatc ggcgagcctg gctttccagg
     6421 tcgcctcgac gtgattgccg atctgcagca cgggacgaag gcgccggctc cgtccgccat
     6481 gcacgaggcc gaggtgtcgg ccatgggcac gcgtcagcaa ctcgaggacc gcagcggttt
     6541 cgccatgcgg cctgaccgac aatacgatcc cttcatcgga ccagttcatg gacctcgacg
     6601 gccgccagcc tcctgccgcg gctcactcct tcgggaactc gagccccatc tcgcgatagc
     6661 gctcgggatc gtcagcccag ttctcccgca ccttcacgtg cagaaacagg tggacctcgt
     6721 gccccagcag cttcgccagc tccttgcggg cctccattga gatctgcttg atcgttgcac
     6781 cgcccttgcc cagcacgatc ttccgctggc tctcgcgctc gacgaagatt gtctgctcga
     6841 cgcgggctga gccgtcgcga cgggtctgcc agtcggtcgt ctcgaccgtc accgaatagg
     6901 gcagctcctc atggagccgc tcgtagatct tctcgcgtgt gatctcggcg gcgagcttgc
     6961 gatcggtcag atccgacagc tcgtccggcg ggtagtgcca gggcccgggc ggcaccgcgg
     7021 acaccaagta atccttcaga tccttgagac cgctgccggt gagcgcggat accatgaaca
     7081 cgcgctcgaa cgggacgcgc ttggtaaggt ccgccgtgag ctcgaggagc cggtgctttt
     7141 ccgccaaccg gtcgaccttg ttcagcgcga gcactttgat gccggccgca tcgccgagtc
     7201 gctgcagaat gcgctcgacg tcctcatcga cacccttcac ggcatcgacg agaagcacga
     7261 cgatgtcggc atccagagca cccgcccagg cggcttccac catggcccgg tcgagccgcc
     7321 ggcgcggccg gaatatgcca ggcgtgtcga ggaaaacgat ctggctttga ccatcgacga
     7381 cgatgccgcg cacgggaata cgcgtcgtct gcaccttgcg cgagacaatg gtcaccttgg
     7441 ctccgacgag ggcattgacg agggtggact tgccagcgtt cggcgcgccg atgacagcaa
     7501 tgaaaccaca gcgcgtgtcg ctttcgggca cttgggcccg cgtggcctct ggctcagcca
     7561 tgtgacggct tcttcagcga attcccagcg tgccggccta tgtcgctgcg cacgccctct
     7621 cgcgcaagca gcgagctcgc cgccgcctgc tccgcctgtc tcttggttgc accttcaccg
     7681 cgggcgggct tgcagccggc gatgtgcacc tcggttacga accggggggc atgatccgga
     7741 ccgctgcgcg aaatctcgat gtattcaggc aggtcgagcc cgcggccctg cgcccattcc
     7801 tgcaacgccg atttgggatc cgcaacgacg tgcggcagtg cctcgaactg cgtggtccaa
     7861 agcgcgcgca cgactttgcg ggccgtctcg aaacccgcct cgatgaaaat ggcaccgagc
     7921 aatgcctcga gggcgtcggc gaggatggtg tccttctcgc gcccgccgct ctcagcctcg
     7981 ctgtcgctca ggatgagatg cgggcccacc ccgatcttcc gggcgacggc cgcgcacgcc
     8041 tgtccacgga ccagcctgtt gtagcggcgc gcaagctcac cctctgtcgc atcgggatgg
     8101 ttctcgtgca gcagctctgc aatcgcgagc ccgagcacgc gatctccgat gaattccaag
     8161 cgctcgttgt ccacgcgcgc gccacgctgt gaccgcacgc tcgcatgggt caacgcccgc
     8221 tcgaacagct cgcggttctt gaaggtgtac ccaaggtgtt tctcgaggtc ggtcgacttc
     8281 ttatgccgtc gcacgtgtgt cttcagcgca ccagattgaa gaagcgatcc caccggatat
     8341 cccacggcca ttgccacggc gtgtagaggc ggaacgcgtt gggatcgtcg aaagctcctg
     8401 agaagaaaat gatctcggcg cggcccacga agttctcgaa cggcacgaag ccgacgcccc
     8461 actgggcacg gctgtccgtc gagttgtcgc ggttgtcgcc catcatgaaa tagtgattct
     8521 ccggcacctt gaatacgccc gtattgtcga acgggccgtt ggggtcggag tcgagcacgg
     8581 tataggttac gccgttgggc agcgtctcct cgtactgcga aatgcggcgc aggacgccgt
     8641 cctcctcgcg agtcacgaag tcgccgatcc gcttcttcgg cacggcttgg ccgttgatat
     8701 agaggacgcc cccacgcatc tggatctcgt cgccgggcag accgatcacg cgcttgatgt
     8761 agtccgtcga gttgtcgcgc ggcagcttga agacggcgac atcaccccgt tgcggcttcg
     8821 aggcgaagat acggcccggg aacaggttga tgccaaaagg aaaggaatag cggctgtagc
     8881 cgtacgaaag cttcgagacg aacaaataat cgcccacgag cagcgtcggc ttcatcgagc
     8941 cggacggaat attgaagggc tggaaaaaga acacgcgcac gaccatggcg agcgcgagcg
     9001 cctgaatgac gatcttcacc gtttcccacc agccactctc tcgctttttg ctggtatccg
     9061 tattcaggct catgtggtgc tccttgggcg gggttgcccc ggtcaatcag gggttccccg
     9121 attccgtctc cggcccgcta taacccttcc aaggggagcg cgcaaaggga taaccgggcc
     9181 gatactgtgc cgttttcaag gcgaactggt gtcgagcgga acgcccgaaa tgatgacgat
     9241 ggcctgcgcc agtggataat cgtcggtcag gctcaagtcg atccgggcgg tgtagcccgg
     9301 cggtgtaatg cgcttcagtt gctcggctgc gccacccgtc agcataaggg tcggacgccc
     9361 agaaggcaga ttgatgaccc ccatgtcacg ccagaacact ccctgccgga agccagttcc
     9421 aagcgccttg gaacaagcct ccttggcggc gaaccgtttc gcatacgagg ccgcgcgctc
     9481 cttccgccga tcagacttgc gccgctccgt ctgggtgaag atgcgctcga ggaagcgatc
     9541 gccgaaccgg gcaatcgtcc gctcgattcg ctcgatatcg acgatgtcgt ttccgagacc
     9601 aagaatcata agaactcagg cgctcctgcg gcctgtgacg cttgcgcgcg cttcgtccat
     9661 cagggcgcgc atgcgccgca cggcggaatg gagcccaccg aaaatggctt cgccaatgag
     9721 gaaatgccca atgttgagct ccacaacttg cgggatgcgc gccacggggg acaccgtgtt
     9781 gtacgtgagg ccatggccgg catggacctc aagacccgca tccgcggcaa tgcgcgctgc
     9841 aacccgcagc cgttccatct cctttgccag ctccctggca ttctcctcga gcgcccgctc
     9901 gcagtaggcg cccgtatgca gctccacgat gtcgctgccg atggctgcgg cggcctcgat
     9961 ctggcgctcg tcaggctcga tgaacagcga aacgcgaata cccgcctcct tgagacgagc
    10021 gacataccct ttcaggtgct cgcgaccgcc tgcgacatcg agcccaccct cggtcgtgcg
    10081 ttcttcgcgc cgctcgggta cgaggcagca ggcgttgggc agatgacgca acgcgatcgc
    10141 cagcatctcc tccgtcgccg ccatctcgag gttgagcggg cgggtcagct cggatttgag
    10201 ccgggcgata tcgttgtccg aaatgtggcg gcgatcctct ctcagatgcg ccgtgatgcc
    10261 gtcggcccca gcctccacgg ccagatgcgc cgcccgcaat gggtcagggt ggcgcccacc
    10321 acgtgcatta cggatcgtcg ccacgtggtc gatgttgacg cccaggcgct gaaacgggat
    10381 gtccaggtgc gagctcattg cgttttctag tgcctcctag agatcgtctc gggcgcacga
    10441 cgcgccaacc ggggcccgct cctttcgctc ccctgcagaa caggacgcgg gcggcccgcc
    10501 ctcaatcgaa cgagcgctca acaatgctca cgaccggctt cgcccgcaag ccggcaatga
    10561 tgcgattcaa atgctccagg tcgaagacct ccacttcgag caacatttcc gtgaagtcgg
    10621 acgcgcggtg cgtcatgcgc agattgtcga tgttgccgtc ggcttccccg atcacctgcg
    10681 cgatctgcgc cagcgatccg ggctcgttga tcgccgtcac cttcagcctg gcgggaaaac
    10741 gctccggatt ttccggtgag atatcccagg tgacgtcgat ccagctctcc ttctcgaaat
    10801 ctttcagccg cggcgaatgg atctggaaga tccggatgcc ttcgccgggc atcatcaccc
    10861 ccacgacgcg atcccctggc accgctccgc cctcctcgaa gctgaccggc agatcgttcc
    10921 gcagcccgcg gatcgggagg cctcccggcc gtgagctcgt atcgctggag ttgccggtaa
    10981 accggaactt caggccgatc accttgctca gattgaacca gccttcctgg ctggttgcgt
    11041 ggctgtggcg gttcttcggc cggtaggact tcgggcgatg ctcggggtcc gcgacgtccg
    11101 gcgcgactgc ccgcaggacg tccgcgaccg gcagctcgct gcggccgacg gctgccaggg
    11161 tgtcctcgat ggtcttctgg cccaggcgat gaagaaccgc tttcagcttc tcgtcgctgt
    11221 actccacgcc agccttgtcg aaccgggccg ccagcaggcg ccgccccagc tcggagtact
    11281 gccgcctcaa cgcgtcccgt gaggcgcggc ggatggcaga ccgcgcccga ccggtgacgg
    11341 caaggcgctc ccaagctgcg ggcggcgtct gaccgctggc ggtaagtatc cgcacctcat
    11401 cgccgttccg cagcatggtc gacagcggca tctgccggcc gttgatcaga gcgcccaccg
    11461 tcgagttgcc gatgtcggtg tggacggcgt aggcgaagtc gatcggcgtt gcgccgcgcg
    11521 gcaaggcgat gaggcgcccc ttcggcgtga agcagaacac ctggtcatgg aagagctcga
    11581 gcttcgtgtg ctccaggaac tcttccgggt tgttgccatc gagcagggtc tcgacgagac
    11641 ggcgcagcca catgtagggg ccgctgtcct tctcgtaggt ggctcgcgaa ccgttgctgc
    11701 cgttatcctt gtagagcgca tgcgcggcga cgccgtactc ggcgacatgg tgcatctctc
    11761 gcgtgcggag ctgcagctcg acgcgctgat ggcgcgggcc gacgatcgtc gtgtgaatgg
    11821 accggtagcc gttctgcttc ggcgtcgaaa tgtagtcctt gaagcggccc gggacagagc
    11881 tccaggtcgt atgcaccacc ccgagcaccc ggtagcagtc ggggatactg cggacaatca
    11941 cgcggaagcc gtagatatcg gacagttgct ccagcgaaat ctgcttgttc tgcatcttcc
    12001 gccagatggc gtacggcttc ttttcccgcc ccgacacctc ggcgtcgata ttctcttcgg
    12061 cgagcttgtt gcgcagcgcc gtctggatct cgtccacaat gccgcggttg cgctcgcgga
    12121 gctgggccag gcgcgcggtc accgtctggt aggcatccgg gttcaaccag cggaacgcgt
    12181 ggtcctccag ctcctcccga agctgctgca tgcccatgcg cccggccaac ggcgcgtaga
    12241 tatcgagcgt ctcctgcgag attcgcgtgc gggtctctgg ccgcatgtag ccgagtgtcc
    12301 gcatgttgtg cagccggtcc gccagcttga ccaacagcac ccggatgtcg ctcgaaatgg
    12361 cgatcaacag cttgcggaag ttttccgcct gctcggcgcg cttcgagacg aggtccaatc
    12421 gccggatctt ggtgagccca tcgacgaggg cgccgatctc gggaccgaag agctggtcga
    12481 tctcggcccg cgttgcctcc gtgtcctcga tcacatcgtg caacagcgcc gtcgcgatgg
    12541 tcgcgtcgtc gagcttcagc tccgtgagga tggccgcgac ctcgagggga tgcgaaaagt
    12601 aaggatcgcc cgaggcgcgc ttctggttgc tgtgcgcctt catggcgtaa acgtaggcgc
    12661 ggttgagcag cgcctcgtcg gccttcgggt cgtagtcgag cacccgctcg acaagctcgt
    12721 actggcgcat catggcctgc gcaccttagt cgagagtacg catccaacgc cggcttgtga
    12781 ggtgatggag aaattcgccg gcgcgcgcgg catgactctc aagcgaactt ctcgatctga
    12841 atcataccca ctactctatg gtccagtgtc cctttggatt ccgcattgcg cccccggccc
    12901 tggcatgagc cctggcgaat ctcagaatcg ggacgcggac ggaatgccgc cgcagcctgc
    12961 gcggcaccac ccccgtggtg gaaaaagctc agcgcccgtt gcggcccccg ccatttccgc
    13021 caatcgcagc agacggctcg gtcggggtca ggctctccat gccgcgcagg agctgctctt
    13081 cggtgaggac atcgacgacg ttgtcgctcg cctgatcgtc gcggccgagc agcggactgc
    13141 gtctctcggg cgccagcacc ggagcgccgg cagcttccgg ctcatccacc tcgacgttct
    13201 tctggatgga gtggatcagc gactcgcgca tgtcatcggg cggaatggtc tgttccgcaa
    13261 tctcacgcag cgcaatgacc gggttcttgt cgttttccgg ctccacggtg atcgggctgc
    13321 cgttcgcgat cgcgcgcgcc cggtgagcag cgagcagcac cagctcgaac cggttgggaa
    13381 ccttgtccac gcaatcttcg acagtgacgc gagccatatt gaaaccctcg tggaagcgca
    13441 acccgaggac gagctaagac cacccgaccc gacctgctgg cctcaggaac ctgcttattt
    13501 acacagaaac cactgagaat caagaattcg gcggacgccg cgacgctgtg gcccgagcct
    13561 ggcgaggagc gttctgcctg gaatgccggt gactggatgc cgccacttgg gcgcaacgtc
    13621 gaggagcggc accaagacga acgcgcggcg atgggcttcc gggtgcggga ggatgatgcg
    13681 gccgcggcga cgtgtgcctg gcggccagcc gtacttgctg ctaccgaaat cgacgatgtc
    13741 gagatcgagc ggcctcggac cccagtggac gccgagacgc cgacccgccc ttctttccaa
    13801 tgacttgagc cttagcagta gggtcgcggg cgcaaccttt gcttttgcca atatgacggc
    13861 attcagatag ggtggctgcc agttgccgcc gataggctca gtctcgtaaa ggggtgagac
    13921 tgcctcgacg gtgatgcgcc gattctgtag ctcctttatc gcgcgacgca gcgttcgtat
    13981 cggagcgccc cacgccccgg caaggttggc gccaatcgag atcagtacag atttcattac
    14041 gattcagaaa aggacctgtc acgcatcaca ttttcgagtt gcagagtaaa gacaatcaaa
    14101 ttgccccatt ccggacgcaa ggtgcgagtc tcagcagttt agtactgtga gccactggac
    14161 gtaaggcacg cgggcggggc gctgcctttg gatcatcgag ttgtgttgga cttctagcgg
    14221 ggagacgatt agagacctgc tcccgaggtg acatatgcat tttcatcctg acgagaagat
    14281 cgcgcttttc atcgatggcg caaaccttta tgcgaccgcc aaggcattgg gtttcgacat
    14341 tgattacaag cgccttctgg ccctgttccg caagcaggga aggctgatca gagcgctgta
    14401 ctacacggca ctggccgagg accaggagta ctcgtcgatt cggccgttga tcgattggct
    14461 ggactacaac ggctatacga tggttaccaa gccgaccaag gagttcaccg actccatggg
    14521 gcggcgtaag gtcaagggca acatggatat cgagctgacg gtcgatgcca tgcgcctgtc
    14581 gcggacggtc gaccacatcg tcctgttctc gggcgatggc gacttcaagt ctcttgtcgg
    14641 tgccctccag caggagggcc ggcgtgtcag cgtcgtctct acgttgcaaa cgcagccgcc
    14701 catggtggcg gacgagctca ggcgccaggc ggaccagttc atcgaccttg cggatctcga
    14761 gcagttcatc tgccgtgatc cggccactcg gccgatccgc aatgatcgtg cctacagcgg
    14821 ccggcgggag ggctaccagc gcccggcaac cgagcccgtg ccgtttcagg atcaggacga
    14881 cctgtccgag gaagtctgag gccggggccc gcctatgcat ggcttatcgg attcggcagc
    14941 atctgagctc agccccgatg ccgccgagcc aagccgagac tgcccgcttt gcccgcggct
    15001 ggtggcctat cggcttgcca accgcgcggc ggagcctggc tggtacaatg ccccggtcca
    15061 ttcgtttggt gacccctccg cgcggctcct gatcgtcggc ctcgcaccgg ggttgcgtgg
    15121 cgccaacaga accggccgcc ccttcaccgg cgatttcgca gggaatcttc tctacgagac
    15181 gctgctcgag ttcgggtttg cggcgggcca ctaccgcgcg gatcccaatg acggcctcca
    15241 gctcatcgac tgcatgatta cgaatgccgt caggtgcgcg ccgccgcaga acaagcctct
    15301 gccttcagaa atcaacacct gccggcgttt tctgagggcc cgaatcggag agctgcaacg
    15361 gctgagggtc atcatggccc tcggccgcat cgcccacgaa agcgtgctgg cagcgctcga
    15421 cgtgcggaaa agcgcctttc cgtttggaca cggtgcgcag catagggttc ggccgggtct
    15481 cgtcctgttc gacagctatc actgctctcg ttacaacacg agcacgggca agctgacgcc
    15541 gcagatgttc aaggaagtca ttcggacgat cgggaactgc ctcggcgatt tggcagatca
    15601 ttaggtcgta gtcgcaggtg gagcagtccc tccgggctcg caatgagccc atgggaaccg
    15661 cgagcatcga tcatcgatta cgaagaggcg tggcgagcga tgccttgcgc cggatcaatg
    15721 acgctgtccg gatcgcgcgc caggctcaga tgcgaatgat acgcattagg gtgacgtgat
    15781 gctcgagatt gcgccggaga aggtcgctca catcatcatc aaggctcgtg agtacgactc
    15841 gaaggtcgat gttcacattc cggacgaggg cagcgggtcg gaaggtgagc ccgacgatgt
    15901 cctggagtcc atgccggacg atacgacacg acccgagctg gtcagcttca tcgactccct
    15961 taatgaggac gagcaggcgc acctggtagc tctggcgtgg gtcgggcggg gcaccttcga
    16021 gccggaagag ttcgacgaag ccgttgccac ggcgcggcag gagaagattg tctcgacctc
    16081 cgagtacctg ctcggcatgc cgctgctcgc agactaccta gcggaaggcc tcgagaaact
    16141 cggcattccc gttgaggata tcgagagcga gatcctctag atcaaaggat catcggcccg
    16201 catcgagcgc gtgtgcgggc acgcctcggc gagtagctgt aggcttatct cattggccat
    16261 gccgacgaat caacggctcg gggaagcact ttcccgagtc aggttcttgc gggcagccgc
    16321 aacccgacga cggttgcgct gcggactccg ggccttcggt tcacttaaat agcccggagc
    16381 gcgacccgtg cccgccatat ctgccaacgt atgctggtcg agcacgctga tgaacgcttc
    16441 cagtgcgtcg tcgagcatac ggttgatcgg cgggcttccg gccttgccct tccgcggaac
    16501 ctcgccgtcc agagcgatat ccataacttc catcgcccgc acaacttcgc cgatccggat
    16561 ctcccgagga tcgcgcgcaa ggcggacgcc gccgttccgc cctctcaccg cctggacgaa
    16621 gccggcgcgc gccaggaggt gaacgatctt gaagacgttc tgcggcgtga tgtcgagctc
    16681 accggcaatg tccgcaacct tgatgagctt gtccggtgca cgggcgccat gaatcaagat
    16741 gcggatcgca tggctggtct gtctggtcag gcgcataacg acacgttctc ctcgccgtgc
    16801 aagcccatcg tgggtctgcg gctcacccgg gagtataccc tagtgttcgt cttgaacgta
    16861 gccgtcgcgt gcgcctcgca tcaattgggc acagcagcac aacgcaggcg caattgttcg
    16921 aaccgcgcga tttgatccgg caacaggttg cgctccgcat ccctgcgcac gaacaccttg
    16981 aacatcgcac cgccttcgcc attgaagaac tggatggagc aggacggtcg cccattgaag
    17041 ggacgggaaa cgaaggcgat ttcggcgcaa ttctcgtggc ggatgtgccc gccgatcggg
    17101 ctgtcgccgt gcagattgaa gtagccgcga ccaacacttc ccggcggaat gcgccctgcg
    17161 cattccaaca cgatatccgg cgtgtgcact atcacaagga cctcacccca ggtctcgagg
    17221 tccgccatga tttctgtgaa gagacccccg gggacgaccg tcctgcaatt ctctggaagc
    17281 gccttgatga cgtccagtgt actcacgccg cattcggcag cgatacgctc gagcacgccg
    17341 tcggggttgg ctgccagctt ttcggccagc agtcgatgct ttgacatgga actgtctcgt
    17401 cctacctggc cggtcatgct gccgcctgct ccgccggggc cttgatttcc tgcaggacat
    17461 ggaagccttc gaattccgga tggccgaaat agagcggccg gatcgcgctg ctgcctgccc
    17521 tagcgtgaga gctgcgaaac tgctcggatt tggtccagtt ctcgaaatcg gcaatggagc
    17581 gccagacggt gtgggaggcg taaagcgtat gatcctcgcg ctcgggccct ttcagcaggt
    17641 gaaaggtgat gaaccctggc aattcatgca ggtagctttc gcggctgagc cacagcttct
    17701 cgaacagctc ctcggagccc ttcgcaacct tgaagcggtt catggcgata tacatggttc
    17761 ggttccttgc ttgatgaagt cactttcagt cgaacgccag cccggcccat attctccgga
    17821 ctggccactc atccgtttct atgcggcccc tattgagccc ccagccgcat cgtcaggccg
    17881 acacgagcag taaacccggg gctgttctcc agatcacgga actgccgata ttcacgatcg
    17941 aacaagttgt cgatgttgac gttgaccgtt gtgttttcat tgacggcata ctcgccaaag
    18001 aggtccacga cggtgtagcc atccgtcgcc agggaggcgg tcgtaacccg cttctgcccg
    18061 gcaacgaagc gcgtgcgggt gccggcgatc aacttttcat cgaaagcccg cataccaagc
    18121 gtcacggtaa tctgatccgc cgggacggta ttgagcgggt cgccggtatc gtcatcctcg
    18181 ccgcggatgc gatgtgctcc gattcccaca aaccagctcc gcgcatcgta catcgcttcg
    18241 aattcgatgc cctcgatcgt ggcattcgcg atgttctgat actgttgggc gtagagcctt
    18301 gctgcctcgg gacacggcgt ctgtggaggc aaagggggtg gcggccaacc aggaatggca
    18361 catccaaatg gcaggtcctt gcgatctacc atatcgatgt agtcgtcgac tttattcctg
    18421 aaggccacga gcttggcccg aaaagcgtct cgaggcctca gtagctcgtc ccatttgaga
    18481 ttgatacctg cttccaagtt gtgcgcaact tcgggcctca aaaagggatt gggcaatata
    18541 tcgaatgccg ggctaggatg aacaccatcc atcagtgtct cggttataga cggcgctcta
    18601 tagccttctg cgtaacccgc gaagaatgtc acaccatgga acggagtgac tcccgccgtg
    18661 atttttggtg atacgcggct tccttcggag ctgtagtcgt cactctcaat ttcgtactgg
    18721 tcgtatcgaa gcgctgcaat gatgtcgatg gtatcgaaaa aggtagcctt gctctggatg
    18781 aaggctcccg cggccgtcct ccgcccgccc ggcgtaagcg cgacggcgct atcgccttgc
    18841 tccgcaaagg tggtcactcg atcctcgaag ccgtctccgc cataggtcag ggcgaccttt
    18901 gcagagccga tttgaaatcg cgatgtattg aaaatgtcaa atccggtggt ctcgacgtcg
    18961 aaactccggt cgtatggatt ggtgactaga cgtgtctgat cgagccgagt gtcgttccta
    19021 tagaccttgg cactgaagtc gagaagggga ttgtccgggc tcaagaacgt ataacccagg
    19081 gtgtattgct tattgtcaac cgttgtatcg aactcggccc cgcgatccgt ctgatcgacg
    19141 aaatccgatc gatagtcgat gaacgtcccg gtgagctggt gccccggcgc caattcccac
    19201 cgagccttga cgaggccgga cttcgtcgta tcgcccgagt tgttaatggt attgccgccg
    19261 ccgtcctcgt agtcgtccga ccagcgaccg ttcagctgac cgaggacgtc gaagttgccc
    19321 acccgtaccg ctccagtgac actgccgagc ttgctgcttg cattggtgcc ataccgggtt
    19381 cgcgtgcgaa cggccgcgta ctcgccaggc agaagaatgt cgtccgcgtc caacaactcg
    19441 aatgcagcaa cgccgccgat ggcccccgag ccgtaaatgg tcgatgtcgg gccacgggtg
    19501 acatcgacgc gcttgatcat ttcgggctcg agatagaacg tcccgtcggc gttatggccg
    19561 ctcttctgga aattctggcg agccccttcg atcagcacat tcacgcggcc gaaatcctgt
    19621 aatccgcgga tgttgatggc ctgggccgta tcgaggccgt tctcctgcgt cgtcacaccc
    19681 gggatcgtgt ccatgatctg cgacacgcta tcggcctgga actgctgatc cagctgctcg
    19741 ttgtcgatgg cactcgatcc gctcagcgct tcgatcgcgc tttcactcat tttggagaaa
    19801 gttagaacga tcccgtccag ctgaaccgtt tgggccgaag cactcgcgct ggcgattgcc
    19861 aatgccgacg ctgaaacgga taggtgcagc gcccatagct tcgcggtgga gcgcccccga
    19921 cgtccgaaca tgctctcccc ctgcctcctg aaaggtcaat gtccccaaac attgcgaaca
    19981 ttggctggct ggcggcggaa ctgcacgctg gcttgctgga tgtcgcgagc gggcgcatgt
    20041 gtggccgaac tcctcagcaa tcgcccgcgc gttggcaagg tatgtcttct tgactattgt
    20101 cgtcaagatt aaacatgtta cacgggactt gcgagcgcga aaggtccgtc cgtgctgcgc
    20161 atccggtatc atcatcgtct cggcagcaaa gatgggtggc acaagtccaa tcaagaatcg
    20221 aaaggcgccg tcggcaaacg acacccaggc gccgaaggcc gtgcgcatgc gcgtgagcga
    20281 cctgttggcc gatacgcgcg aagccattct ggagcacaac ggtcaggaat accgcctccg
    20341 gattacggca aacggcaaac tgatcctgac gaaataggcg ataagcatca tgagccgcac
    20401 cattccaggt tttgtgactt tggccatcgt cgccgccgtg ctcggggcta cggcttccat
    20461 cgcggctgag gtcgtggatg cgaggggtga ggtgatcaaa gcggacaacc cctcgcgaat
    20521 cctgagcatc ggtggcgata cgacggaaat cgtctatgct ctcggtttcg gctcaaacgt
    20581 cgtggcagtg gatacgagca gccagttccc gccggaggtg ctggccaaga agaaggtcgg
    20641 atatatgcgc gcactctcga cggagggcgt gctgtcgaca ggcgcgacac tgatccttgc
    20701 caatgcgcac gccgggccgc ccgaggtcat tcgcgcgctg gcaacgtcct cagttccctt
    20761 ggtcatgctt cccgagaatg caagcccgga gagcctcatc gagaaggtga agctcgtcgg
    20821 gcgcgcgctc ggcgcggaag cgaaagcgaa cgacctggcg gctcagctca acggccaatt
    20881 cggctcgctg gaaacggttc ggtcgaagat cgaaaggccc gttcgggccc tgctggtgct
    20941 cagcgtcgac ggcggccgcg ccctggcggc cggcagagga acgacggccg atctcatgtt
    21001 ggaactcgcc ggtgccacga atgcagccgc ggacctcaac ggctacaagc ccgtgagcga
    21061 cgaagcgctg atcgaaatgg cgccgcaggc catcgtcgcg gtgcgcagag cacccgagga
    21121 ggagatcgcc gccgacatcg cggcgctccc tggattcaag gctttgggag ccggccggaa
    21181 agtcccgatc atcacgatgg atgcagctta tttgctcgga ttcggaccgc gcgcgccacg
    21241 ggcggccggc gagctcatgg cccagctcta tcccgacttg ccaaaccagg tggctcggtg
    21301 acgatcgaat ccctggacct tccggccgtc gaagacggca tcgatcgcca tgggctgggg
    21361 cgtcgcctga tcctggcggg cctgctggcc ctggggttgg cagcagtggc ctccgtgagc
    21421 gtgggcccga caggccttag cctcttctca ctgcctcgag cgatttcagc ggcgctatcg
    21481 ggcagtgatg cggctgccga tgtacatgcc cgcctggtcc tgctcgaggt aagactgccc
    21541 cggacgatcc ttgccgcgtt cgtcggcgcc tcattggcgg tcgcaggcgc catgatgcag
    21601 ggactttttc gaaatccgct cgccgaccct gggctgatcg gtgtttcctc tggcgccgcc
    21661 ctggcagcgg tggcgaccat tgcgttgggc ggcggcttcg ccggcccctg ggtgcgagca
    21721 ctcggcatct gggcgctgcc ggtttcggcg ttcctcggcg gtttcttgac aacgatggtg
    21781 ctgatgctca ttgcgcgcaa gcacggcacc accgccatcg caacgctctt gttggcggga
    21841 attgccctgg cggcgatcgc gggcgccttg accgggctga tcgcatacgt gacggacgac
    21901 cgtgagctca gggacttgac gctctggtcc atgggatcgc tggcaggcgc gagctggcag
    21961 aaggtgatgg ccatcgcgcc ttttgccctg atcctgttcg tgacgatccc gcgcttcgtt
    22021 cgcgcattga atggaatgct gctgggagaa gcggaagcct tttacctcgg catcgaggtc
    22081 gaacgtgcga agttctggat cgtggtcttg acggcggcgg ccgtcggagc agcagtggcc
    22141 gtcgccggaa cgatcggctt cgtcggcatc gtcgtgccgc atcttgtgcg cctgactgcc
    22201 ggccccgacc accgcatcgt gctgcccgga tcggcgctgc tcggggcggt cctggtggtc
    22261 gtggccgaca ttgcggcacg catgatcgtg gcgcccgccg agctgccgat tggaatcgtc
    22321 atggctgtta tcggcgcacc ggttttcctg cacctggtca tccgccgcgg gggaggtttg
    22381 gctgcgccat gatccgggcc gaggcggtca cttacgctgt tggcaatcgt agcttgctga
    22441 gccgcgtcga tctcgatttg caggccggac gtctcattgc agtgatcggc cccaatggcg
    22501 ccggcaagtc gacattgctt cgccttctga caggcgagct caagcccacc ggcggccgca
    22561 tactgatcga tggacaggat ctggcgaccc tttcggccga tcagttggcc agacgccgcg
    22621 ccgtcgtctc ccagcatacg gcattggcct ttcccttcac ggcgctcgaa gtggtgctgc
    22681 tcggcatact ggtgccgggc ttcgaggcag attgcagcgg cacacgccgc attgcgaggg
    22741 acatgctggc gcgcttgggg atggaacatt tggcagatcg gacctacacg acgctctccg
    22801 gcggcgagcg tcagcgcgtg cacatggctc gggcgctctg tcagctcgcg tgcggccgtg
    22861 tgcctgccga gaagacggcc attctggtcg atgagccgac atccagtctc gatatcggcc
    22921 atcagctcct cgtgctcgat gagctgacgc gtcagaagaa tgccggccgc gctgtgctgg
    22981 cggtgctgca cgatctcaat cttgcttcaa cgtatgcgga cgagatgctg cttctggccg
    23041 atggggcggt cctggctcgc ggatcgccgg ccgaggtcat gaccgatgaa cgcctgtcca
    23101 tggcctttgg ctgcgatgtt cgaacgaact gcgttccgcg gaatggtgcg ccctatctgc
    23161 tgccacagtc ctgcaccgcc gtcgccacgt aatcatggct cggccggctc gctgcgcttt
    23221 acgatctcac gacacgagca cagcgtggtt gtcccattgc cagtgcgtgc tgctggtcat
    23281 cgtggcgcct tgcgacgggc tcgctccaga tgcgatctcg cccttccccg tcgtcagcag
    23341 gaatccgccc actgacggcg caacgccgga gacatcgacc aggcttcgct ccccgagcac
    23401 ccggccggtg gcgacgtcga tgaacagaac gatcccaccg cgcgagcccg tgagggccgc
    23461 gatctctccc gaagcatcga cggcaacgga gctgatgtag ccgcgtagcc gtggcgacag
    23521 gtccgctgca agctcgacgg tcttcggcag ccctccgaga cgatggcgca agatgagcgg
    23581 cgtgcccgcc gtgacatcgc ccttgaattg gccgccgatc accaccatcc cgccggcgcc
    23641 gacatcgaga tggcgcagcg aaagttgtct caacccctct ggcagctcgt ggcgctcgac
    23701 aagatcgccg gtgatcgtat cgacataggc gagcgagggg gacatcgtgg cacggttgag
    23761 ttgcgtccgg ccgccgccga tcgcggggtg ggtctcgatc ccgccattgg cgatcacaag
    23821 cgtacgcccg tccttcatca gggcgatgtc gtgcgggccg acgccatgcg actccatctc
    23881 gccgatccgg cgaaagccag ccgtcgcatc gtaaagaccg atacagccgc ggcccgcctc
    23941 gaaatcgttc tccgtcgcga aaaggatgcg cccgtcgcgg gaaaagatgc catggccgta
    24001 gaagtgccgg tcgggcggtg tcgtgaagcg gatgggcttc cagcctttat cccggcgaaa
    24061 ggccactgcg aaattgcccg gtcggcgggc gaacgccacg caggtccggg tcacggggca
    24121 gaccgtcaca tcgtgcccgc gggccggcag ggccacggca ttcacatctg ttccgtcggg
    24181 ccggaaaatg gccgctgaga aggcgccgtc cggggaacgg cgcgcggcgg caaacagcgg
    24241 ctcgtccgcc gcgcgggttt cggccggcgc accttgcgcc gccaacccgg acgccaggag
    24301 acctccggcg cccgccagga atgctctgcg atcaatcgcc atcggatgca ttgaacccca
    24361 tcggcaagtc ggtcgtgagg ctgaacgccc tttccgcctg aaaccgcgcg cttttcagcg
    24421 ggaagccgag cgcgatggcc tcgtcacgct gcgaagagcc aaagaagtcg gggaccttgg
    24481 tgagcggcgt ggacaagcgc gtcaggagcc gcagctcgaa cctgacctgc ttcatcgcct
    24541 gctcggccgc gggcgaacct cgcgccttgg cggttcgcgt cgtctccttc gccagtccgc
    24601 cgtcctcgat caaagagcgc aagccggtga tccgtgcccc gatgaatgcc atcgtgaggt
    24661 ccgagcgggc gaagggaccg ggaaggtcgc gcccgcgcac ggccaaaccg aggggccgca
    24721 tgagctcgac gtcgcgcaca cgctcgatgt tgtcgagata ggcgcggatc aacgcatagg
    24781 tcgtttcgga aggctggatg taggtcggat tgcctggacc aggggtcagc cagcgttccg
    24841 accagatacc accttcagac caggccgcct ccacctcgcg cgcaatattc accaggttgc
    24901 cggcgatcgc ctgggcgtaa gagcacctga acgagctgtt ctcgcccggc tccgctggcg
    24961 tcgcgatgag ctgctccaac gccggcaggc cctgcacacc gatgctcgat tgggcaagct
    25021 tggcggcatc ccggtactcg gaggggcttt ccttcagcgc acgagcgacc tggcggcgcg
    25081 cgataccctt ccggtcgagc cagaaaaaga cgcggtcata tcggttctct gcggcgaggg
    25141 gtccgaagtt gagatgcgcg acacgacccc aggcgaggac tgccttccgg aagactggcc
    25201 tcaggcgggc atagcggaat tgcggctgct tctcgcaaga gcccgttgcc tgctgaagct
    25261 ccgcaaaggc gctcgtgagc cgcctgaacc ctggccgaat gtgcttctcg agcgctgtgc
    25321 gggccaccag cgcgtgatcg aagtccgttg cggcccgctg ctcggccgag gccgccggtc
    25381 cggctccgat aaaggcgacc aatgccaggg ctgtcagttt cgaccggaac gtcggcatgg
    25441 gtgatcggcc ttcagagtga attgagaaac gcgatcagcc tcgcgcggtc ggccgggtcg
    25501 agcgctgcga acgcatcgcg cgccgcctgc gcctcacccc catgccaaag aatcgcttcg
    25561 gcgagattac gtgcgcgccc atcatgaagg aaataggtgt ggccgctgac cgtctcggtg
    25621 aggcctatgc cccaaagcgg ggccgtgcgc cattcccggc cgtcggcacg cccctcgggg
    25681 cgcccgtcgg caaggccctc acccatgtcg tgaagcagga ggtccgtgta cggccagatg
    25741 tgctgtccgg ccaagtgggc gtcagacgcc tccgtgcccg tcatgaagct cgggcgatgg
    25801 catgccgcgc agccgatcga atggaacagc gctttgcctg cgagaacatc gggagcgtcc
    25861 gggttccggc gtggcggaac ggccagattg cgggcgtaga atgccgtaag cttcaagagc
    25921 tgcgtcttga attcctctcc aggtgccttg gcggttcctc catgcggcgc tgcaagacag
    25981 gtcggttgcc gctccgtgca atcgccgctg ggcttcggaa ccaggacgga gccaaggccc
    26041 atgtcgcctg ctgcggcctc tgccgtctgc tgcagcacgg tcggcgcgcc ggccttccac
    26101 ccgaaccgcc cgagcatgag cttgttgtgc tcgatcgacc agacgcggtt cgcccgaccc
    26161 gagatgccat cgccatcgcg atcgtccggg tcctcgccgg ccagaatatc tgcctccgga
    26221 atcgcttcga gcaggccaag cccgatcatc ggcggggcca cgcgcggcga gaccatcgtc
    26281 tcagggtgca tcggcccata acccaaatcg gtgatggtgt aggtgggctt gcgcaggtgc
    26341 acgacctcgc catcagcgag cgtaacgggc agctcctcat atgagatgtg cacgtggccc
    26401 tcgcccttat gccccgcaat cgccagatcc tggagctgcg ttccgtaggt cggctcgggg
    26461 atcacactgg cgcggccgga ggcgagcagc tcgcggtcgg catcggtttg cggagggatg
    26521 gacagccgca ggagcatgga taccgcgttg tcatccggcc agtttccatt gggaggatgg
    26581 ccgcggccgt ccttcaggtg gcaggtctgg cagctacgcg cgttgtagag cgggcccagc
    26641 ccgtcggatg acttcgtcga ggccggcgcc gagacccaga gcttgcggaa aatggcgttg
    26701 ccgatcttga agtcgagctc gcgggcgaac cccatgttgg cggacgagtg cgagaaggcg
    26761 ttcgtgttgt cggtcgagcc cagagacgtg gcctctccgc caggatggcc ctcagcagga
    26821 tcgaaggcag caggaaacgc ctccgcaccg gtcgaatttt ccgaccagga caggccggca
    26881 ccgaccagca ggaatggcag gctggccgcg gccgcgagga aaccttgcgc tgatcgcagc
    26941 cggcgctgtt ccttttgctt ctcgcgagag aacatcgtgt cacatgggcc ccggacgatg
    27001 acggtcctgc ggaggcctac tgaaagacct tgctcggatt gtcgaggctg tccgagccct
    27061 cgaactgcac cgcgccgagc tcgagggcgg cgatggccct ttcgaactcg cgggtctggg
    27121 cgatgagcgc ctgaagcgcc gcctcgacca cggcattgcc ctcgtcattg gcctcgccga
    27181 tcatctggtc gtaagcctcc accgtctctg cccgcttctt cagggtttca aattgcgcca
    27241 gcgactcgtc gagcgcatcg cggacgcggg catcgatctc ggcgtccttg gccttgacaa
    27301 ggtccgacac gctcggcccc gagatcacgg acccatcgat ccgctcatat tttccgaagt
    27361 agacgttccg aatgccgatg aggtcatagt agtgcgagtt gtgcgtattg tcggagaagc
    27421 agtcgtgctc ctcttcaggg tcattgagca gcagccccag cttcatgcgc tcgccagcaa
    27481 gctcaccgta ggagagtgat ccaagcccgg tgaagatcgc gacgagtcct tgtgtttcgt
    27541 cagctgtcag agccttgcga gccgcgccgt ccgccttcca ttgctcaacc atccaagtca
    27601 ggtcgtcgac cagaagctcg gtgacggtcc ggagatattg cgcgcgccgc tcacagttgc
    27661 cccctgtgca attggcggtg tcgtagtcgc tcgccggccg cgcgcccgcg ccagcgtccg
    27721 tgccattcag gtcctggccc cagagcagga actcgatcgc atggtagccg gtcgccacat
    27781 tggcctcgac gccgcccgcc tcgtgcagcg tgtccgacag gagttccttc gtgatcttgg
    27841 acgcatcgac catacggccg gagatcctca cgctcggatt cgcaatgatg ttcgcgacat
    27901 aaagcgggtt ttcctccgac tccgtaccgt agtcgccgtc aacgtagtcg atgagtccct
    27961 catcaagcgg ccaggcattc accttgcctt cccagtcgtc gacgattcgg ttgccgaaac
    28021 ggaagacctc ggtctgctga taggggacac gggccgcaag ccaagcggcc tttgcctccg
    28081 caagacgctc ttccgtcggc tccgcgagga acgcctctat ttttgcctgc agcgccttgg
    28141 ctgtggtcag cgagtcctcg tacccagcca gcgcgatatc ggcataggtg tcgaggacgt
    28201 cccgcggtgc cggcgccgct gccatcgcac tcgtcgccat gaccgagagc gcgcccagcg
    28261 tcagaaacca gtcgaccagc cgtctcatat cgcctccttc gattcggaat agcccgaagc
    28321 cgctcccaac gcagacttaa agatacgcat cagccctgca catttcgtta caggcaaaag
    28381 agcttcgctc tgctcttctg agcacaccgt tcagcatacg ttctcggttt gctgcatcgc
    28441 gccggtgctt cgaccaacgg ctgaacaggc agatagccaa gccgaaccct ggaagggcaa
    28501 tcattattga ttgtttttgt caagaaacac cgggggctcg tccgtgctgt ggccggggca
    28561 atctcgtcag cgaggtgacg agctgcagcg gtgccagtct cggcatgagg gcgggcggat
    28621 ggcgaggcgc ttgagaagcg cgaccgtacg gagacagtga aggaacggtt aatgcctgac
    28681 ggcggtcaac ttgccgaaaa tcgagacttc ggccaaaagc cgcgccgctc ttctcatcct
    28741 cagcgatttg cggctcttcc cgggattggg tgctcgccga aatcgacgac cgagcgcttg
    28801 agaatgcggc cggaagcgtc gacggccagc cgtgcgcgcc ggttcttgta gaagaacgtc
    28861 agccggtatt ggccttcgtg ttctccctcg ccgcgctcgc aagcgctcgt gatctcgttg
    28921 ttgcgcatca aggcctggac gcctcgcggc ggcacgccga gcaattcccc tagaagcgtc
    28981 gcatcgatgc aaaagccgtc atcggttcgt tccagtgaca tctgcatac
//
LOCUS       DASVDU010000211         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_451930, whole genome shotgun sequence.
ACCESSION   DASVDU010000211 DASVDU010000000
VERSION     DASVDU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451930"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VF226_13590"
     CDS             <1..421
                     /locus_tag="VF226_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075069.1"
                     /translation="RHTSDRVAVMYLGEIVEIADCDALFEAPRHPYTRSLIEAIPVPV
                     PGARRARRLLEGDPPSPAAPPPGCRFHTRCPFAADFCRIKRPALRTLSPGHAVACHLA
                     EELPAAVSADLAVAGSEAGRRRVRLFAEAQRKSASVS"
     gene            440..1942
                     /locus_tag="VF226_13595"
     CDS             440..1942
                     /locus_tag="VF226_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075070.1"
                     /translation="MKRGTLAASIAALGLTLVSLPALAADIRIGLQEDPDILDPHEAR
                     TFVGRIVFNLLCDKLVDTNEKLEFVPRIATSWSFSDDGKTMTMKLRSGAKFHDGTTLD
                     AAAVKANIERAKTLQTSRRKSELKSVESVEAPDAETVVFKLSAPDATLLAQLSDRAGM
                     LLSPASFDKPVGPHPVCSGPYKFVERVQNDRIVLTRFPDHWEADKYAFDNVIFRIIPD
                     TTVRLANLRSGELDMIERLAPSDVKSAKADPALQVVSIAGLGYQGITINVGNGDRSKT
                     PLGQDKRVRQALSLAIDRVAANEVVFEGIMTPSAQPFPPASPYHDQDFKVPARDVEKA
                     KALLKEAGAEGISFEMQVSNSPVELQLAQVIQAMAAEAGITISLKATEFATMLKEQTA
                     GNYQATRVGWSGRVDPDGNIHQFMTTGGGINDSKFSHPEVDKALNAARSVYDVEERKK
                     HYAAAMKILRDEIPIVYLYHPAWVWAMSSKLKGFVPNPDGMIRLAGVTLQ"
     gene            2034..>2554
                     /locus_tag="VF226_13600"
     CDS             2034..>2554
                     /locus_tag="VF226_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020810969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075071.1"
                     /translation="MWALILNRVLVAIPTLFIVTVFVFALQRMLPGDPLLVMAGEERD
                     PVVLEQLRHLYGFDQPLPVQFLAWLREVFSGNFGASLRTGEPVLKLIGQTLPVTIQLA
                     IASIVIALAIGIPMGILSAVRKGTWIDAVASMVSLSGLSIPNFWLGIILILVVAVNLQ
                     WLPASGFVSVFEDP"
BASE COUNT          433 a          852 c          817 g          452 t
ORIGIN      
        1 ccgccataca tcggacaggg tcgccgtgat gtatctcggc gagatcgtcg aaatcgccga
       61 ttgcgatgcc ctgttcgagg cgccgcgtca cccctacacg cgctcgctca tcgaggcgat
      121 cccggtgccg gtgccgggcg cccggcgggc gcgccggctg ctcgaaggcg acccgccgag
      181 cccggccgcg ccgccgccgg gatgccgctt tcacacccgc tgcccgtttg ccgccgattt
      241 ctgccgcatc aagcgtccgg cgttgcgcac cctctcgcca ggccacgccg tcgcttgtca
      301 tctcgccgaa gagttgccgg cggccgtgtc cgccgacctc gccgtggctg gaagcgaggc
      361 aggccgccgg cgggtccgct tgttcgccga ggcgcaacgc aagtcagcat ccgtgagctg
      421 agcatggagg agacgaccca tgaaacgagg aacacttgca gccagcatcg ctgctctggg
      481 ccttacgctc gtcagtctgc cggcgcttgc cgccgatatc cggatcggcc tgcaggagga
      541 cccggacatc ctcgatccgc acgaggcgcg cacattcgtc ggccgcatcg tgttcaacct
      601 gctctgtgac aagctcgtcg acaccaacga gaagctcgag ttcgtgccgc ggatcgccac
      661 gagctggtcg ttctccgacg acggcaagac gatgacgatg aagctcaggt ccggtgcgaa
      721 gttccacgac ggcacgaccc tcgatgctgc tgctgtcaaa gccaatatcg agcgggccaa
      781 gacgctccag acgagccggc gcaagagcga gctgaagtcg gtcgagtccg tcgaggcccc
      841 ggatgcggag acggtcgtct tcaagctgtc ggccccggac gcgaccctgc tcgcccagct
      901 ttcggaccgc gccggcatgt tgttgtcgcc cgcgagcttc gacaagccgg tggggccaca
      961 cccggtctgc tcgggtccct acaaattcgt cgagcgcgtc cagaatgatc ggatcgtgct
     1021 gacccgcttc ccggaccact gggaggccga caaatacgcc ttcgacaacg tgatcttccg
     1081 catcattccc gacacgaccg tgcgccttgc gaacctgcgc tcgggcgagc tcgacatgat
     1141 cgagcggctg gcgccgagcg acgtgaagtc ggccaaggcc gatcccgcgc tgcaggtcgt
     1201 ctcgatagcg ggcctcggct accagggcat cacgatcaac gtcggtaatg gcgaccggtc
     1261 caagacgccg ctcgggcagg acaagcgggt gcgccaggca ctctcgctcg ctatcgaccg
     1321 cgtggcggcc aacgaggtgg tgttcgaagg catcatgacg ccgtcggcgc agcctttccc
     1381 gccggcgagc ccctaccacg accaggattt caaggtgccg gcgcgtgatg tcgagaaggc
     1441 gaaagcgctg ctgaaagagg ccggcgccga aggcatctct ttcgagatgc aggtctcgaa
     1501 ctcgcccgtc gagctgcagc tcgcccaggt gattcaggcg atggccgccg aggcgggaat
     1561 cacgatatcg ctcaaggcga cggagttcgc gacgatgctg aaagagcaga ccgccggcaa
     1621 ctatcaggca acgcgcgtcg gctggtctgg gcgcgtcgac cccgacggca acattcacca
     1681 gttcatgaca acgggtggcg gcatcaacga ctcgaaattc tcacaccctg aggtcgacaa
     1741 ggcgctcaac gcggcccgca gcgtctacga cgttgaagag cgtaagaagc actacgcggc
     1801 cgcaatgaag atcctgcgcg atgagatccc gatcgtctat ctctaccatc cggcgtgggt
     1861 gtgggcgatg tcgtccaagc tcaagggctt cgtgcccaat ccggacggca tgatccgcct
     1921 cgccggcgtg acgctccagt agcgcacatt tgcatcaggg tcggcgcgcc gaaacgtgcg
     1981 cgccggccgc aatcgtgttg gccgagacct gaaatcggag ccggactagg ccgatgtggg
     2041 ctttgattct caatcgcgtt ctggtcgcga tcccgacgct cttcatcgtc acggtgttcg
     2101 tgttcgcctt gcagcgcatg ctgccgggcg atccgctgct cgtgatggcg ggggaggagc
     2161 gtgatcccgt cgtattggag cagttgcgcc acctttatgg gttcgatcag cctctgccgg
     2221 tgcagttcct cgcctggctg cgcgaggtgt tctccggcaa tttcggggcc tcgctcagga
     2281 ccggcgagcc ggttctcaag ctcatcgggc agacgctgcc ggtaacgatc cagctcgcga
     2341 tcgcctccat cgtgatcgcg ctcgccatcg gcatcccgat gggcattctg tccgccgtgc
     2401 gcaaggggac atggatcgac gcggtcgcca gcatggtctc gctgtcgggg ctctcgatcc
     2461 cgaacttctg gctcggcatc atcctcatcc tcgtcgtcgc ggtgaacttg caatggctgc
     2521 ccgcctcggg gttcgtgtcc gtgttcgagg accc
//
LOCUS       DASVDU010000212         6366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_894490, whole genome shotgun sequence.
ACCESSION   DASVDU010000212 DASVDU010000000
VERSION     DASVDU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894490"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VF226_13605"
     CDS             <1..662
                     /locus_tag="VF226_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX7075072.1"
                     /translation="RTYEEITSLLISGQLTAAWICGYPFVSYREELALVAVPVWRGQP
                     LYQSYLIVDADRHASSLDDLKGDVHAFSDPNSNSGYLVTRAALAKRQWRPEEFFRHSF
                     FTYGHRNVVRAVASGLAQSGSVDGYVWEVISEIEPELTGRTRVLERSELLGFPPVACP
                     RRSQDTPETLALKRALLDMNSDPLGTEVLKELRLDGFSDAKPSVFDGIAAKLALVRSL
                     G"
     gene            662..2152
                     /locus_tag="VF226_13610"
     CDS             662..2152
                     /locus_tag="VF226_13610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7075073.1"
                     /translation="MGVRRPKFTRLNPLQWPLALRVPVLAALMMVTVSAVVTDRVLTR
                     LIDTQNRHLTELSDAYLDGISSAVIPHVLREDIWEVYDALDRAAQRYEGLDIAWTTVT
                     DPVGRVIASSQPRAFPLQTELPDPVRSRLNGASDSATYEDHGVFHVKRELVHQHRVVG
                     AIYAEMRIGTLIEERREVLATLIASNTTLTLLFAIIGYITVRRMMRPIRVLSESMRSG
                     AAGSVTPVPVELIGRPESEFGQLFRRYNSLVQAVAERERLLKSLAEEERLAALGRLAS
                     GMAHEINNPIGGMLNAVDSLKRHGDRAAVRETSIRLIEKGLLGIRDIVRSSLATYRHR
                     QDERPLTATDVDDLALLLQPEIKRKNLQLEWRNAMDREIFVPVGGVRDAALNLLLNAC
                     AATEPNRTVSFEATATQDGIELEVRDQGPGMPRVILDYINAPLSQPAPVAPRSGLGLW
                     MVKRFAHEVGGQLEATTRACGGTSVRLKIPERKKPFAKTQELSHVA"
     gene            2142..3479
                     /locus_tag="VF226_13615"
     CDS             2142..3479
                     /locus_tag="VF226_13615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7075074.1"
                     /translation="MSLEGRVIGLIEDDPIMGESLVQSLTLEGAYVDWWQSGSEAIRG
                     LKATSPDLVVCDIRLPDTTGDRLFSEIAQTRSAPPFLFMTAFGDIDEAVRLMRAGAGD
                     YVAKPFDLETFMDRARSLIRAWSQVRPEGTLGASEAMKRVEQMLRRVAPLTSPVLLTG
                     ETGVGKEVCARFLHGISPRADRPIIAVNCAALPTDLMESELFGHERGAFTGAHAQHKG
                     YAERAGGGMLFLDEIAELPLPLQAKLLRLIEERAFTRVGGERKIPFKARLVCATNCDL
                     NAAVRDGRFRLDLLHRINTVSIEIPALRQRPEDIPTLMELFFDQFSAELNSDVRGFAS
                     LTIDAALEHSWPGNVRELRNRVERALALSYGQWIMPTDLFPEQATGRDGETVAPLATL
                     SEAREVAERRQIQRALRESAGRVTRAAELLGISRTTLWEKMRRLGIGSGDEEE"
     gene            3648..4169
                     /locus_tag="VF226_13620"
     CDS             3648..4169
                     /locus_tag="VF226_13620"
                     /EC_number="1.20.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050611 - arsenate reductase (azurin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (azurin) small subunit"
                     /protein_id="HEX7075075.1"
                     /translation="MKGCNKLVDVGRRQFLRGGAVATVGAVASAVVPSTSKAKPLPAR
                     VQYPSNRLANISELEVNEPLHISYPDEDSPGVLLKLGTPVDGGVGPDRDIVAFSTLCP
                     HKGFPLVYVAEDKTLSCPGHYSLFDCEKGGLQIMGQATQNLPQFALRVDDKGDIYAEG
                     VDELIYGRLSNVL"
     gene            4185..>6366
                     /locus_tag="VF226_13625"
     CDS             4185..>6366
                     /locus_tag="VF226_13625"
                     /EC_number="1.20.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050611 - arsenate reductase (azurin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (azurin) large subunit"
                     /protein_id="HEX7075076.1"
                     /translation="MAFKRHIDRLPIIPADAKKHTVVCHYCIVGCGYHAYTWPVNKQG
                     GTEPSQNAFGVDLSKQQQAETEAWYAPSMYNIVQQDGKDVHLVIKPDKGCVVNSGLAS
                     ARGARMAEMSYSRARATQQQRLTHPMVWRYGQMQPTSWEDALDLVARVTVAVIKDQGE
                     DGLFVSAFDHGGAGGGYENTWGTGKLYFGAMKVKNIRIHNRPAYNSEVHATRDMGVGE
                     LNNCYEDAELCDTIVAVGCNPLETQTNYFLNHWIPNLRGTSLDKKREQLPDEEHASGK
                     IIIVDPRRTATIAACEAEAGKENVLHLPIEPGTDMVLFNAWLTYIAEKGWQDKDFIAA
                     STKDFDKALAANKTTLAEAAKICGVPEADIEKAAQWIAAPKNGKRRRTMFAYEKGLIW
                     GNDNYRTNAALVNVALATGNIGRPGGGCVRMGGHQEGYARPSDAHVGRPAAYVDKLLI
                     EGKGGVHHIWGCDHYKTTLNAHQFKQVYKKRTDMVKDAMNAVPAGDRAAQVAAIVEAI
                     KAGGLFAVDVDIVPTKIGEACHVWLPAATSGEMNLTSMNGERRMRITERYMDPPGEAM
                     PDCLIAARIANHMERVLREIGDDKYADQFKGFDWKTEEDAFMDGYHKVAKGGEHVTYE
                     RLRAMGTNGFQEPAVGFEGGKIVGTKRLYADGKFDTKDGKASFMEAKWRGLQAPGKKE
                     QKAKFKYLINNGRTNLVWQNAYLDQDNEFVMDRFPYPFIEMNPED"
BASE COUNT         1344 a         1908 c         1941 g         1173 t
ORIGIN      
        1 ggcgcaccta tgaagagatc acgtcactgc tgatctccgg ccagctcacg gccgcctgga
       61 tctgcggata tccgttcgtg agttatcgcg aggagctagc ccttgtggcg gttccagttt
      121 ggcggggaca gccgctttat caatcgtatc tcatcgtcga tgccgaccgt cacgcctcca
      181 gcctggatga tctcaagggc gatgttcacg cgttctcaga cccgaacagt aattctgggt
      241 acctcgtaac tcgcgccgcg ctcgcgaaac ggcaatggcg gccggaggaa ttcttccggc
      301 actcgttctt cacctacggt caccgcaacg tggtcagagc cgtggcttcg gggctggcgc
      361 agagcggaag cgttgatggc tacgtatggg aggtgatctc cgagatcgag ccggagctga
      421 ccgggcgtac ccgggtgctc gagagatcgg agctgctcgg gtttccgcct gtggcctgtc
      481 ctcgccgatc ccaggacacg ccggagacac tggctctcaa gcgtgcgctg ttggacatga
      541 acagcgatcc gttgggtacg gaggttctga aggagctgcg gctcgacggg ttcagtgatg
      601 ccaagccatc ggtgttcgat ggcatcgcag cgaagctcgc cttagtacgg agcctcggct
      661 gatgggcgtt cgacggccaa agttcacgag gctcaatcct ctgcagtggc ccctggcgct
      721 acgtgtgccg gtgctcgctg cgttgatgat ggtgacggtg agtgctgtcg tcaccgatcg
      781 cgtgctgacc agactgatcg acacgcagaa cagacacctc actgagctct ccgacgccta
      841 cctcgacggg atctcatccg ccgtcattcc gcatgtactg cgtgaggaca tctgggaggt
      901 ctacgatgcg ctagaccgcg cagcgcagcg ctacgagggt ctggatatcg cttggacgac
      961 tgtcaccgat cctgtcggac gcgtcatcgc atcgagccaa ccgcgagcct tcccgctgca
     1021 gaccgaactt ccggatccgg ttcgctccag actcaacgga gcttcagata gcgccaccta
     1081 tgaggaccat ggcgttttcc acgtaaagcg cgagctcgtc catcagcaca gggttgtcgg
     1141 tgcgatttac gccgagatgc gcatcgggac tctaatcgaa gaacggcggg aggtcctggc
     1201 aacactgatt gcctcgaaca cgacgctcac actgctgttc gccatcatcg gctacatcac
     1261 ggtccgccgc atgatgcgcc ccattcgcgt actgtcagag agcatgcgat ccggcgcggc
     1321 aggtagcgtt acgcccgttc cggtcgagct catcggccgg ccggaaagcg aattcggtca
     1381 gcttttccgc cgttacaatt cgcttgtcca agccgtggcc gagcgcgaac gactgctaaa
     1441 aagtctggcc gaggaggagc gacttgccgc tctaggacga ctggcttccg gaatggccca
     1501 tgagatcaat aatcccatcg gcgggatgct gaatgccgtc gattccctca aacgccacgg
     1561 cgatcgcgcc gcggtacgag agacttcgat tcggcttatc gaaaaaggcc tcctcggcat
     1621 ccgggacatt gttcgatcaa gcctagcgac ctatcggcat cggcaggacg aacgaccgtt
     1681 aacggcgacc gacgtcgatg accttgccct gctactgcag ccagagatca agcgcaagaa
     1741 cctgcagctt gagtggcgga atgctatgga ccgggagatt tttgtacccg ttggtggcgt
     1801 acgggatgcg gcactaaatc tgcttctcaa cgcctgcgcc gcaacagagc ccaatcgaac
     1861 agtgtcgttt gaagctacgg caacgcagga cggaatcgag cttgaagttc gtgaccaagg
     1921 tcctggaatg ccacgcgtca ttctggacta catcaacgcc ccgctttcac agccagcccc
     1981 ggtggctcca cgcagcggat tagggctctg gatggtgaag cgctttgccc atgaagttgg
     2041 cggccagctt gaagcaacca cccgcgcttg cggaggcacg tcggtccggt tgaagatccc
     2101 cgagcggaaa aagcctttcg ctaagacaca ggagctcagt catgtcgctt gagggccgcg
     2161 taataggcct cattgaggac gaccccatca tgggcgaaag tctcgtgcag agcctcacgc
     2221 tcgaaggggc ctatgtcgat tggtggcaat ccggctctga ggcgatcagg ggtctcaagg
     2281 caacaagtcc tgatcttgtc gtgtgcgata tccgcctgcc cgatactaca ggtgatcggc
     2341 tattcagcga aatcgcacaa acgcggagcg cgccgccctt cctgttcatg acggcctttg
     2401 gtgatatcga cgaggcggtc cggcttatgc gcgccggtgc tggcgactac gtcgcaaagc
     2461 cgttcgatct agagacgttc atggaccgag cgcgaagtct gatccgggca tggtcacagg
     2521 tgcggcccga gggaacgctc ggcgcttccg aagccatgaa gcgcgttgag cagatgctgc
     2581 gccgcgtcgc accgctgacg agccccgtcc tgctcaccgg tgagacaggt gtgggcaaag
     2641 aggtgtgtgc gcggtttctc cacggcatct cgccgcgcgc cgataggccg ataattgctg
     2701 tcaattgcgc agcattgcct acggatctca tggaaagcga gctgttcgga catgagcgag
     2761 gcgcctttac gggagcacac gcacagcaca agggctacgc cgagcgcgct ggcggaggca
     2821 tgttgtttct cgatgaaatc gcggagctgc cgcttccact acaggccaag cttctgcgac
     2881 tcatcgaaga acgggctttc acgcgcgtgg gtggggaaag gaagatccca ttcaaggcgc
     2941 ggcttgtatg cgcgacaaac tgcgatctaa acgcggcggt gcgtgatggg cgcttccgcc
     3001 tcgatcttct gcatcgcatc aacaccgttt ctatagaaat tccggcgctg cgccagcgac
     3061 cggaagatat tccgaccctg atggagctgt tcttcgatca attcagcgca gaactgaact
     3121 ccgatgtacg cgggtttgca tcgctgacca ttgatgctgc cctggagcac tcatggccgg
     3181 gaaacgtccg ggagctgcgt aacagagtgg agcgggcctt agcattgtcc tacgggcaat
     3241 ggatcatgcc gaccgacctc ttcccggagc aagcaacagg tcgcgacggc gaaactgttg
     3301 cgccgctggc aactctcagc gaagcgcgcg aagtcgctga aaggcgtcaa atccagcgcg
     3361 cccttcggga gtccgccggt cgcgtgacgc gagcggcgga actcctcggc atatcacgga
     3421 cgacactctg ggaaaaaatg cgtcggttgg gtatcgggtc tggcgacgaa gaggagtgat
     3481 gttcaggttt ccgaacgtgg caatcaatcg cgttgggata tccgaacggt ttacacgagt
     3541 ccgccaaccg caagctattg aaacttcacg cattctgcgc gccaccaaca ttggcacatc
     3601 gcttgcgatg ctttccctgt ctctgaagcg atagggagag cgatccaatg aaaggctgca
     3661 acaagctggt cgacgtcggg cgtcgccagt tcctgcgcgg cggcgccgtg gcgacagtag
     3721 gggccgtcgc atcggcggtc gttccatcta cgagcaaggc aaagccgctg ccggcccgcg
     3781 tccagtatcc ttcgaaccgg ctggccaaca tcagcgaact tgaagtcaac gagccgctgc
     3841 acatcagcta tcccgacgag gacagcccgg gtgttctcct taagcttggc actccggtcg
     3901 acggcggcgt cggccccgat cgcgacatcg tcgccttctc gactctgtgc ccacacaagg
     3961 gctttccgct agtttacgtc gctgaggaca agacgctgag ctgccctggc cactactcgc
     4021 tgttcgactg cgagaagggc gggctccaga ttatgggcca agcgacgcag aacctgccgc
     4081 agttcgcact ccgggtcgat gacaagggcg acatctacgc tgaaggcgtg gacgagctca
     4141 tctatggccg cctgtcgaac gtgctctgag ggaggaactg atccatggca ttcaagcgcc
     4201 acatcgaccg tctgccgatc attcccgcag acgctaagaa gcataccgtg gtctgccatt
     4261 attgcatcgt gggctgcggc taccacgcct acacgtggcc cgtgaacaag cagggcggca
     4321 cggagcccag ccagaatgcg ttcggcgtgg acctatcaaa gcagcagcag gccgagaccg
     4381 aggcgtggta tgcgccctcg atgtacaata tcgtgcagca ggacggaaaa gacgttcacc
     4441 tcgtcatcaa accggacaag ggctgcgtcg tgaactcggg gctcgcttcg gcacgcggcg
     4501 cccgcatggc ggagatgagc tactcacgcg cccgcgcaac ccagcagcag cgcctcacgc
     4561 acccgatggt ctggcgctac ggccagatgc aaccgacctc atgggaggat gcgctcgatc
     4621 tcgtcgcccg tgtgacggtc gccgtcataa aggaccaagg cgaggacggg ctattcgtct
     4681 ctgccttcga tcacggtggc gccggcggtg ggtacgagaa cacctggggc accggcaagc
     4741 tctacttcgg cgcgatgaag gtgaagaata tccgcattca caatcgccca gcctacaatt
     4801 ccgaggtgca cgccacccgc gatatgggag taggtgagct caacaactgc tacgaggacg
     4861 cggagctctg cgacacaatc gtggcggtgg gctgcaatcc gctggagacg caaaccaact
     4921 acttcctcaa ccactggatc ccgaacctgc gcggcaccag cctcgacaag aagcgcgagc
     4981 agttgcccga cgaggagcat gcctcaggca agatcatcat tgtcgaccca cggcgaactg
     5041 cgacgatcgc agcttgcgag gccgaggccg gcaaggagaa cgtgctgcac cttccgatcg
     5101 agcccggcac ggacatggtg ctcttcaatg cttggctgac ctacattgct gagaagggct
     5161 ggcaggacaa ggatttcatc gctgcctcta ccaaagattt cgacaaggcc ctcgccgcca
     5221 acaagacgac gctggcggaa gcagcgaaaa tctgcggcgt tccagaagcc gacattgaga
     5281 aggcagcgca gtggatcgcg gcgccgaaga acggaaagcg tcgccggacg atgttcgcct
     5341 acgagaaggg gttgatctgg ggcaacgaca attatcgcac caacgcggcc ctcgtgaacg
     5401 tggcgcttgc caccggcaac atcggccggc cgggcggcgg ttgcgttcgt atgggcggcc
     5461 accaggaagg ctatgcgcgt ccttccgatg cccacgtcgg acgtccggca gcctacgtcg
     5521 acaagctact gatagagggc aagggcggcg tgcaccacat ctggggctgc gatcactaca
     5581 agaccacgct caatgctcac cagttcaagc aggtctacaa gaagcgcacc gacatggtga
     5641 aggatgccat gaatgcggtg ccggcgggcg accgtgccgc gcaagtcgct gctatcgtcg
     5701 aagccatcaa agcaggcggc ctcttcgctg tcgacgtcga catcgtgccg accaagattg
     5761 gcgaggcctg ccacgtttgg ctgcctgccg ccacatcggg tgagatgaat ctcacctcta
     5821 tgaacggtga aagacgcatg cgcatcaccg agcgctacat ggacccgccg ggagaagcca
     5881 tgccagactg cctcatcgct gcccgcatcg ccaaccacat ggaacgtgtt ctgcgcgaaa
     5941 tcggcgatga caaatatgcc gatcagttca agggcttcga ctggaaaacg gaagaagacg
     6001 ccttcatgga cggctaccac aaggttgcca aaggcggtga gcacgtcacc tacgagcggc
     6061 tgcgcgccat gggcaccaac ggttttcagg aacctgcagt tgggtttgag ggcggcaaga
     6121 tcgtcggtac gaagcgcctg tatgccgatg gcaagttcga caccaaggat ggcaaagcga
     6181 gcttcatgga ggccaagtgg cgtggcttgc aggctcccgg caagaaggag cagaaggcaa
     6241 aattcaaata cctcatcaac aatggtcgca cgaacctcgt ctggcagaac gcttacctcg
     6301 accaggacaa cgaattcgtg atggatcgct tcccttaccc gttcatcgaa atgaacccgg
     6361 aggaca
//
LOCUS       DASVDU010000213        11675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_388058, whole genome shotgun sequence.
ACCESSION   DASVDU010000213 DASVDU010000000
VERSION     DASVDU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388058"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF226_13630"
     CDS             complement(<1..392)
                     /locus_tag="VF226_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX7075077.1"
                     /translation="MPWRLLKSQYWQESRLDPSAVSPAGARGIAQFMGPTWAEVSRAM
                     GWGLVSPHDVRYAIEAGAYYDSRLYRQWRAPRPETDRISLMLASYNAGLGSILNAQRR
                     CNGAPLYAEIIACLPQVTGRHSSETIGYV"
     gene            complement(451..660)
                     /locus_tag="VF226_13635"
     CDS             complement(451..660)
                     /locus_tag="VF226_13635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075078.1"
                     /translation="MAGLSILASIVRVLLALGTIYLALTALDRLNGFPFTRAQEVMNA
                     DPRAVAHYRGLRFLAACILAGLIFS"
     gene            complement(993..1208)
                     /locus_tag="VF226_13640"
     CDS             complement(993..1208)
                     /locus_tag="VF226_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075079.1"
                     /translation="MLTPRIYPRGSAASTQEIDAIAERAAQKALQIVYAEVGKSVLRK
                     AAWLAGAVVIGLAMWLAGKGHFPGAGS"
     gene            complement(1292..1597)
                     /locus_tag="VF226_13645"
     CDS             complement(1292..1597)
                     /locus_tag="VF226_13645"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7075080.1"
                     /translation="MADRVVFVPDEGLAIWNGREVELTRQEAQLFGLLVRMQPHVTST
                     MRLAAMIWPAQLPVMPENHVRVVACRIRTKLAGFPISLVARRGYGYRLAGKVEIAPE"
     gene            complement(1690..1884)
                     /locus_tag="VF226_13650"
     CDS             complement(1690..1884)
                     /locus_tag="VF226_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075081.1"
                     /translation="MSDHPLTEEQIDAIAERAAQKALQIVYAEVGKSVLRKAAWLAGA
                     VVIGLAMWLAGKGQFPGTGG"
     gene            complement(1881..2210)
                     /locus_tag="VF226_13655"
     CDS             complement(1881..2210)
                     /locus_tag="VF226_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075082.1"
                     /translation="MPATRLKAVCVRLINAGVVLAALAVPWPASGQSLTCIPRDLVLK
                     NLADNFQEAPVGVGLASNGVLLELLTSPDGSTWTVILSAPNGMSCPLAAGRDWQDLVA
                     PERGDPA"
     gene            complement(2287..2868)
                     /locus_tag="VF226_13660"
     CDS             complement(2287..2868)
                     /locus_tag="VF226_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075083.1"
                     /translation="MRAILAALALLLFPASALAGWQEVDVIGVRTDNSKAPTVIFINT
                     YTGTGPAPGVWHQIDVTALGVPADAKAAFLSGILIISHGTTPQSCDLQVALRAPGDPM
                     SAGNVIGQTLETAVGGGQRSNMSSWVPLVGGKFEFQWNRNTYGPWPSECSYGINLSLQ
                     AYGRAVDIGGGPSSTTVDDVLHRLAEILAQQYQ"
     gene            complement(2868..3863)
                     /locus_tag="VF226_13665"
     CDS             complement(2868..3863)
                     /locus_tag="VF226_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075084.1"
                     /translation="MTALLYDRVWETTTTTGTGSYTLDGAKSAAYRAFGDVLADNDYV
                     EYFVRDSDASNWERVWGQYDAGNNTLTRNLIESSTGSLIDWGSGTKEVYIAPPAKGLN
                     DEGGIYVGESRPAWMLANRLWLKPSTSTLYWYDGTGDREMGLVSGGSFIAKTPTTARG
                     DIIRRGATIDERLAIGSAGQVLTSDGTDPVWSNLYTAGTWTPVLTFQTPGNLSVAYST
                     QVGTYVKVGSLVTATVNIQTSSFTHTTASGILQVTGLPFTSKNVSNQFALGSCAWQGI
                     TKASYTDVVCRIINNVTYIDFMISGAGQSTVQVVAGDVPSGGTVRVQTTICYEAA"
     gene            complement(3860..4663)
                     /locus_tag="VF226_13670"
     CDS             complement(3860..4663)
                     /locus_tag="VF226_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075085.1"
                     /translation="MAKNLFWLDPTASDSATVTGLGTLASSMPAHNVLNLAPGSRARW
                     TSTTGAAIKLDLGTSASWDTIAIIGHNAGASDTYRVRADDTEADVSGGSAALDVSSRS
                     FWPASGKPAGRDVYHSFYRHSSVLTYRYVRIDFTIASQPFDVRRIMIGEAEQPTCNYE
                     YGSGDGYTPTGIVERSIGGHTWGLYGRPLNVKQLHLVARSKSELYGALHKMLRNRSVE
                     RDMFICLDEDEDDYLADMMLWGHIENPGIPAVQAFDFWRYSLNFVEQAP"
     gene            complement(4664..7348)
                     /locus_tag="VF226_13675"
     CDS             complement(4664..7348)
                     /locus_tag="VF226_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075086.1"
                     /translation="MAVHFVTPKSLTLSGTGSYFDVDLSDDVPSGATGVLLRAKNSSS
                     SNLSYGLRKNGSSDDLYGLLRRNDPPNPAHVWEFVGVDSSRIFEVKREGTGIGLDLIG
                     WFDAGTVMLTDAVELSLSTVNAYETVDLSDTLPAGAVAAILLFGFTGNTNVYARATGA
                     THSYPGRKSGRMPFLTGVDSSRRCDIYRSSSSDRVWLLGYLMDGYTVHATPEDDTPTS
                     TFTWEDKSLPSGAIGACWWGYNTLGDLPAMGVREDGSSYNAYGNLCYTAATLISKAAS
                     EVVEINRTYTSDRVYRLGYFTAGADDHVVQPVSIESAPEFSAASITPAGNHTVSPISI
                     VSASEFTAPSLGFSVDQRPEQILLIAHPFDATESVTLDGPPVPTGEGVFDDAEFTADG
                     AEVSVYWGDGRGASFVSGSSDTPANQPFLPRLQSAYQFSVEAFSGALSGGARPSGAAT
                     ESSGAVEVLNTDGGIDDVLDYAWDGRSVELYRVRSGAALADARLCLRGTIERLTWSDG
                     KIVLGLRDRHALFQRPLITATYGGTGGADGDANVIGVPKRQGYGPVINAEGVLINSAL
                     LIWQIHDGRIFAVDAVRDKGVTLTFSADYDDYDELAAATIPARQYATCLAEGMIRLGA
                     SPDGRVTCDFRGDAAGGVYVDTAADIFRRIVTTRITGANIADPDGLDTASFDALNAAQ
                     PAAIQYYTAGENPTVADVLAQIMTTVGGWYTFTREGLLQVKRLEAPSSPSFTLDRTNI
                     SSSDRFSAVREPPSWRERVEWGRPWVIQSGDDLAGEVTAEDRDRWSRRHYAEWRDSSV
                     RGAHRLARTVETPSLFAYELDAQTEAERLGALWGVARDVITVRPSGQIFLRWVGDVGS
                     IDLEDADGNARYGLPKNAVVVALYENARNGETRLVMVG"
     gene            complement(7360..7755)
                     /locus_tag="VF226_13680"
     CDS             complement(7360..7755)
                     /locus_tag="VF226_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075087.1"
                     /translation="MTISIHDDVLDKGPNEIKTNATTVTICAGAPTTLSDATTNLGTG
                     TGKKLADVTVDSSDWSIADGSPDGRTLTFTQQTGVTVDVSGDADHLAVVDASRLLLVI
                     PLSATVAVTSGNTMTINSFTYRIPDPVAA"
     gene            complement(7778..10837)
                     /locus_tag="VF226_13685"
     CDS             complement(7778..10837)
                     /locus_tag="VF226_13685"
                     /inference="COORDINATES: protein motif:HMM:NF018497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HEX7075088.1"
                     /translation="MADTTRVVRIVVDASGAQQGSSQVTSALERIDRQRQRTQSGLQA
                     LAAANASASARMIAGMSAVNNASLRMIEGMSRAGQAANLLDRQIGVAANTNRRFGSAI
                     QQAGFQVGDFAVQIASGTNPLRAFVQQGTQLISMFGPWGAIIGAAGAVVGALAMSFLD
                     LEGATDAATSAQERHNELMERAKQLTEELTASTVNLSNARRIEGQNRLALMRQELRDL
                     ERLEEERRRAERGQFGSYGDEIAGQEVSVVTEGRPSNARIDQLKREIRDLEVLLWGDQ
                     ISRGTVPGMQRTVKATKDLGDAADRASPKVRTLGDILADLNQKAIANEAREYADAVRL
                     IERTVESGLTDQQRYQKQIDDLNRALEKAAERGYTLSIEAQQALNRAMREANPAVQAA
                     NRAYEEQRRERERLAEQEAELMRRPFENALEGIQDSFTDFFQSVFDGGIDSFRDLASG
                     IKSIFTRLAAEIAALLVFRPVVAGILGGVGLGGIAGGMGLMPTSGGGLPGGVGIGTGS
                     IIDAAGGIGRALAGGFSGLNAWLFGLPAVGGSHPIAATAGLLGSGGTFLGSTFLAEAF
                     LPGLGALLPGLISGNVKQAGLGLGGALLGTAIGGPIGGAIGGILGNLVGDLFGGKKGI
                     PQVTQNLLFQGGQLVPGAPWSANNGGSMVAGTRTLGQSVADAVNQIVADLGGSILEGA
                     TGGYVKVKQTGKGTRYFAGLGSYGSGISLGAGDDPQAIAGLVISGILQEAARAGNLTG
                     VSDSVLTVLNQGKVTKGSVRSLEQLNEDIAFAQLYDQLTGIAKPVTQAEQAIKELNKQ
                     FADAKRRAEDLGLAVGPLVEAQAKAIEKLTTDFNASIQDQILAITNPTALALKQLEEA
                     QRARLEEAKKLGADLVEVERLSALERKRVLETSGLRSFFDEITFGGLSGASPGASLEG
                     ARAAFEAAAASGDIAGIQTYGRAYLETARAFHASGAGFQDALARVQNVVGSHLAANSN
                     MSVVSAVNDVGSLIAASNNLLAQLRAEMSALREENRELREQMLALAS"
     gene            complement(11168..>11675)
                     /locus_tag="VF226_13690"
     CDS             complement(11168..>11675)
                     /locus_tag="VF226_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075089.1"
                     /translation="CRGQCRLAGPLRLVRKLHPEDRPRGRVMASPYELFGTDAKAEKD
                     GIILDYGAFWLRIARAGGANKKFARVLDAKLQPYRRQLQAGTMEDELANRLMAEAYAE
                     AVILAWGSKEHGDGKMIGRDGEAIEFSRENVIRLLLDLPDLFADIQNQAGRVANFRAA
                     QIEDAAGN"
BASE COUNT         2012 a         3888 c         3737 g         2038 t
ORIGIN      
        1 acgtaaccga tcgtctcgga tgaatgcctg cccgtcacct gcggcaggca ggcgatgatc
       61 tcggcgtaga gcggcgcgcc gttgcagcgc cgctgggcat tcaggatcga gccgaggccc
      121 gcattgtagc tggcgagcat cagcgagatc cggtccgtct caggccgggg cgcgcgccac
      181 tggcgataga gccgggagtc gtaataggct cctgcctcga tcgcgtagcg cacgtcatgc
      241 ggcgagacga gaccccagcc catcgcgcgg gaaacctccg cccacgtagg ccccatgaat
      301 tgcgcgattc cccgcgcccc cgccggcgag acggcggacg ggtccagccg gctctcctgc
      361 caatactggc tcttgagcag gcgccacggc atgccgggca gccaccgctc cgccgcccgc
      421 tcgatctcgg tgtcgtaccg atccgggaat ctaggagaag atgaggccgg cgaggatgca
      481 ggcagccaga aagcgcaggc cgcgataatg agccacagcg cgcgggtccg cattcatgac
      541 ctcctgagcg cgggtgaagg ggaagccgtt gagccggtcg agcgcggtga gggcgagata
      601 gatcgtgccg agcgccagga gcacgcggac gatcgaggcg aggattgaca gccccgccac
      661 gtctagtagt tccatgtctc acctgggttg ttgtgcgggg agtcccgctg gggtaggatt
      721 gttgtcttgc tgcggcggca cggatggacg tgcgatccgc gtaggtcgaa ggcggttgta
      781 acgcggctta agtgcgaagc ctcatgatct ggcggtcctg atcagtcgcc cggaggcaac
      841 ctcgccaaga ccagccgaga aatcggcccg tagcaaatta ctcgcctgtc gggagaccgc
      901 aggccccccg ctcagctccg gctggcgggc catcatcccc gccggttcgc ccggcaggac
      961 agggccgctc atgtagggcg gccctttctc gcctagctgc ccgccccggg gaaatgcccc
     1021 ttcccggcga gccacatggc aagaccgatc accaccgcgc cggccagcca tgcggctttc
     1081 cgcaggacgg acttcccgac ctcggcatag acgatctgga gagccttctg ggccgcccgc
     1141 tccgcgatcg cgtcgatctc ctgggttgac gcggcggagc cgcgggggta aattcgtggg
     1201 gtcagcaacg cgccgtcgtt cgccgccgct cagcggctag gggtagctac ccgaacgaat
     1261 tgctgggggc cgtccttcgg ggcggccctt ctcactccgg cgcgatctca accttcccgg
     1321 ccagcctata cccgtagccc ctgcgcgcca cgagagagat ggggaagccg gcgagcttcg
     1381 ttcggatgcg gcaggccacg acgcgcacat ggttctctgg catgaccggg agctgcgccg
     1441 gccagatcat ggccgccagg cgcatcgtgc tcgtcacgtg cggttgcatc ctgacgagca
     1501 atccgaacag ttgggcctcc tgccgggtaa gctcgacctc tcgcccgttc cagattgcca
     1561 atccctcgtc cggtacgaaa accacccgat cagccatgtc gtcctgttca gcatgaggca
     1621 gagaaggaac aggagcgggc gcatccatca tttcatcctc agcagcgtga gagcgcgccg
     1681 cgcggcatat caaccccccg tccctggaaa ctgccccttc ccggcgagcc acatggctag
     1741 accgatcacc accgcgccgg ccagccatgc ggctttccgc aggacggact tcccgacctc
     1801 ggcatagacg atctggagag ccttctgcgc ggcccgctcc gcgatcgcgt cgatctgttc
     1861 ttccgtcagc ggatggtcgc tcatgcaggg tccccccgct caggggccac aagatcctgc
     1921 cagtcccgtc ccgctgcgag cgggcagctc atgccgttcg gcgccgagag gatgaccgtc
     1981 catgtgctgc cgtcagggct cgtcagaagc tccagaagga cgccgttgct cgccagaccg
     2041 accccgacgg gagcctcctg gaaattgtcc gcgaggttct tcaggaccag atccctagga
     2101 atgcaggtca gcgattgacc ggaggcaggc caggggaccg caagagccgc caggaccacg
     2161 cctgcgttaa tcaatcgcac acacacggct tttagcctcg tcgcaggcat gggcttgctc
     2221 cttacccgag cgggttcatg ctccggcccg gcgttgctga tcccagcgtc gggccgcctc
     2281 cattgattac tggtattgct gggccagaat ctcggccaga cgatgcagaa cgtcgtcgac
     2341 ggtcgtggag ctcgggcctc ccccgatgtc aaccgcccgc ccgtaggctt gcaggctgag
     2401 gttgatgcca taagagcact cggacggcca tgggccatag gtgttgcggt tccattggaa
     2461 ctcgaacttg ccgccgacga gaggcaccca ggaggacatg ttcgaccgct ggccgccgcc
     2521 cactgcggtc tcaagcgtct ggccgatgac gttcccggcc gacatggggt cgcccggcgc
     2581 tctcagggca acctgcagat cgcagctctg cggcgtcgtg ccatggctga tgatgaggat
     2641 gccggagagg aacgccgcct tcgcatctgc cggcacgcca agcgccgtca cgtcgatctg
     2701 atgccagacg ccaggcgccg gacccgtccc ggtataggta ttgatgaaga tgaccgttgg
     2761 cgcttttgaa ttatcggtgc gcacaccgat gacatcgact tcctgccagc cggcgagagc
     2821 cgacgccgga aagagcagca gggcgagcgc cgcgagaatc gcacgcatca ggccgcctca
     2881 tagcagatgg tagtctggac ccgtaccgtg ccgccgctcg gcacgtcgcc cgccacgact
     2941 tgcaccgtgc tctggccggc gccggaaatc atgaagtcga tataggtcac gttgttgatg
     3001 atgcggcaga ccacgtcggt gtagctcgcc ttggtgatgc cctgccatgc acatgacccg
     3061 agtgcgaatt ggttggacac gttcttggac gtgaagggca ggccagtgac ctgcaggatg
     3121 ccgctcgccg tcgtatgggt gaaagaagag gtctgtatgt tgaccgtggc cgtgaccaga
     3181 ctgccgacct tcacataggt tccgacttga gtggagtagg caacgctgag attgccaggt
     3241 gtctggaacg tgagaacggg agtccatgtc ccggccgtat agagatttga ccatacagga
     3301 tcggtcccgt ctgacgtgag aacttggcca gctgagccga tggccagccg ttcgtcgatg
     3361 gtcgcgccgc gcctgatgat gtcgccgcgc gcggtcgttg gggtctttgc gatgaagctg
     3421 ccgcctgaaa cgaggcccat ctcacggtcg ccggtaccgt cgtaccagta gagcgtcgat
     3481 gtcgagggct tgagccacag ccgattcgcc agcatccagg cgggccggct ctcgccgacg
     3541 tagattccgc cctcatcgtt caggcccttg gccggcggcg cgatgtagac ttccttggtg
     3601 ccggagcccc aatcgatgag cgatcctgtg gacgactcga tcagattgcg cgtgagcgtg
     3661 ttgttgcccg cgtcgtactg cccccagacg cgttcccaat ttgacgcatc ggaatcgcgg
     3721 acaaagtatt cgacgtaatc attatcggcc agcacgtcgc caaacgcgcg gtacgcggcc
     3781 gatttagcgc catccagggt ataggaaccc gttcccgtcg tggtcgtggt ttcccaaacg
     3841 cgatcataca gcaatgccgt cacggagcct gctccacaaa attgaggctg tatcgccaga
     3901 agtcgaatgc ctgaacggcg ggaattccgg ggttctcgat atgcccccac agcatcatgt
     3961 ctgcgagata gtcgtcctcg tcctcgtcca ggcagatgaa catgtcgcgc tccacggagc
     4021 ggttgcgcag catcttgtgg agcgcgccgt aaagctccga cttggatcgg gcgaccagat
     4081 gcagctgctt gacattgagc ggtcgtccgt acaggcccca ggtgtgtccg ccaatgctgc
     4141 gttccacgat gccggttggt gtgtagccgt cgccggaccc gtactcgtaa ttgcaggtcg
     4201 gctgctctgc ctcgccgatc atgatgcggc ggacatcgaa gggctgcgag gcaatggtga
     4261 agtcgatccg gacatagcga taggtcagga ccgacgagtg cctgtagaag gagtgataga
     4321 catcgcgccc ggccggcttg ccggatgcgg gccagaacga gcgcgacgac acgtcgagcg
     4381 cggctgagcc gccggaaacg tccgcctcgg tatcgtccgc cctgacgcga tatgtgtcgg
     4441 atgcgcccgc gttatgcccg atgatcgcga tggtgtccca cgatgcggat gtacccaggt
     4501 cgagcttgat cgccgcgccc gtggtcgagg tccatcgagc gcgcgagccg ggcgcgagat
     4561 tcagcacgtt gtgcgccggc atggacgacg cgagcgtgcc gagccccgtc acggtcgcgc
     4621 tgtcgctcgc cgtgggatcg agccagaaca gatttttcgc cacttaaccc accatcacca
     4681 gacgggtttc gccgttgcgc gcgttctcgt acagggcgac gacgacggcg ttcttcggca
     4741 ggccgtatcg agcattgccg tctgcgtctt cgagatcgat ggacccgaca tcgccgaccc
     4801 agcgcaggaa tatctgaccg gagggccgga ccgtgatcac gtcgcgggcc accccccaca
     4861 gcgcgcccag ccgctccgcc tcggtctgtg catcgagctc ataggcgaac aacgatggcg
     4921 tctcgacggt gcgtgccaga cgatgcgcgc cgcgcacgga tgaatcgcgc cattcggcat
     4981 aatgccgtcg cgaccaccgg tccctgtcct cggccgtgac ctcgcccgcc aggtcgtcgc
     5041 cgctctggat cacccatggc cgtccccact cgacgcgctc gcgccatgac ggcggctcgc
     5101 gcaccgcaga gaagcggtcg gacgatgaga tgttggtccg gtcgagcgtg aatgacgggc
     5161 tggacggcgc ttccagccgc ttgacctgca acagaccctc tcgcgtgaac gtgtaccagc
     5221 cgccgaccgt cgtcatgatc tgcgccagga catccgcgac ggtcgggttt tcgccggccg
     5281 tgtaatactg gatcgcggca ggctgcgctg cgttgagtgc atcgaacgat gcggtgtcga
     5341 gcccgtccgg atcggcgatg ttcgccccgg tgatgcgcgt cgtcacgatc cggcggaaaa
     5401 tatcggcagc cgtgtcgacg taaacgccgc cggccgcatc gcctcggaaa tcgcatgtca
     5461 cgcggccgtc cgggcttgcg cccaaccgga tcatgccttc ggcaaggcag gtcgcatatt
     5521 gccgagcggg gatggtcgct gcggccagct cgtcatagtc gtcgtaatcg gccgagaacg
     5581 tgagtgtcac gcccttgtcg cgaaccgcgt cgaccgcgaa tatccggccg tcgtgaatct
     5641 gccagatcaa cagggcggag ttgatcagca cgccctcggc attgatcacc gggccgtagc
     5701 cctgtcgctt aggaacgccg atcacattcg catcgccgtc cgcgccgccg gtcccgccat
     5761 aggtcgcggt gatcagcggg cgctggaaca gcgcgtgacg gtcccgcaga ccgaggacga
     5821 tcttgccatc cgaccatgtg agccgttcta tggtcccccg caggcacagc cgcgcatcgg
     5881 cgagcgccgc gccggagcga acccgataga gttcaacgga tcggccgtcc caggcgtaat
     5941 ccagaacgtc gtcaatgccg ccatcggtgt taagaacctc gacagcgccg ctcgattccg
     6001 tagccgctcc gctcggccgt gcaccgccag aaagcgcgcc ggagaacgcc tccacggaga
     6061 actgataggc ggactggagg cgcggcagga acggctgatt ggcgggcgtg tcgctcgatc
     6121 ccgacacgaa cgatgcgccg cgcccgtcgc cccaatagac cgagacctcc gcgccatcgg
     6181 ccgtgaactc tgcatcgtca aacacaccct cgcccgtggg aactggcggg ccgtcaaggg
     6241 tcaccgattc agtggcatcg aacggatgcg cgatgagcag gatttgttcg ggacgctggt
     6301 cgacggaaaa cccgagagag ggcgcggtaa actccgacgc ggaaacaatc gagatcggcg
     6361 agacggtgtg atttccggcc ggcgtaatcg atgcggcgct gaattccggt gcgctttcaa
     6421 tcgagacggg ctggaccaca tggtcatccg cgcccgccgt gaaatacccc aggcgataca
     6481 ccctatcgga cgtatatgtc cgattgatct cgacgacttc cgaagcggcc ttggaaatca
     6541 gcgtcgcggc ggtgtagcag agattgccgt aggcgttata ggacgagccg tcctcccgca
     6601 cgcccatcgc cggcaagtcg ccgagcgtgt tgtatcccca ccagcatgcg ccgatggcgc
     6661 cggacggcag gctcttgtcc tcccaggtga acgttgaagt tggcgtgtcg tcctcaggcg
     6721 tggcatggac ggtatagccg tccatgagat agcccagcag ccataccctg tccgacgacg
     6781 agctgcggta gatgtcacag cgccggctgg aatcgacgcc agtcaggaac ggcatccgtc
     6841 ccgacttgcg gccggggtag gagtgggtgg cgcctgtcgc ccgcgcatag acatttgtgt
     6901 tccccgtgaa gccgaacagc aggatcgcgg caacggcacc agccggcagc gtgtctgaca
     6961 ggtccacggt ctcataggca ttgaccgtgg acagcgacaa ctcaacggca tcggtcagca
     7021 ttaccgtgcc agcgtcgaac cagccgatca ggtcgaggcc gatccccgtc ccctctcgct
     7081 tgacttcaaa tatcctagat gaatcgacgc cgacgaactc ccagacatgg gcgggattgg
     7141 gcgggtcatt acgccgcagg aggccataaa gatcgtcgct cgatccgttc ttcctcaggc
     7201 cgtatgacag gttgctggag ctggagtttt tggcccggag caaaacaccc gtcgcgccgg
     7261 acggcacgtc gtccgacaga tcgacatcga aataggaccc ggtgccggag agcgtgaggc
     7321 tcttcggcgt tacgaaatgg accgccatgg ctcagcggct cacgctgcga ctggatcggg
     7381 aatgcgatag gtgaacgagt tgatcgtcat ggtgttgccg gacgtgacgg ccaccgtcgc
     7441 ggagagaggg atgaccagga gcaggcggga cgcatcgact acggccagat ggtccgcgtc
     7501 tccgctcaca tcgaccgtga cgccggtctg ttgcgtgaat gtcagagtac gcccgtccgg
     7561 cgagccgtct gcaatggacc agtcgctcga atccaccgtc acatcggcca gcttcttccc
     7621 cgtgccggtc ccaagattcg tcgtcgcatc cgacagcgtg gtcggtgcgc cagcgcagat
     7681 cgtgacggtg gtcgcgttcg tcttgatctc gtttggacct ttatcgagca cgtcgtcgtg
     7741 gatactgata gtcatgggtg ttcctgtgtc ggtaggatca agaggctagc gcaagcatct
     7801 gctcgcgcag ctcgcggttt tcctcgcgca acgcggacat ctcggcccgg agctgggcaa
     7861 ggaggttgtt ggatgcggcg atgagcgagc ccacgtcgtt tacggcagag accacggaca
     7921 tgttgctgtt cgccgccaga tggcttccga ccacgttctg aacgcgagcc agcgcatcct
     7981 ggaagccagc accagaggca tggaacgcgc gggcggtctc cagataggcg cgaccgtagg
     8041 tctgaatgcc ggcaatatcg ccggatgccg ccgccgcctc gaatgccgcg cgcgcgccct
     8101 caagcgatgc accgggcgag gcaccggaga gcccgccgaa cgtgatctca tcgaagaacg
     8161 agcgaaggcc gctggtctcc agaacgcgct tgcgctccag ggccgacagg cgctcgacct
     8221 cgaccagatc ggcgccgagc ttcttggctt cttcgagccg agccctctga gcttcctcca
     8281 gttgtttcaa cgccagcgcg gtcggattgg tgatcgcgag aatctggtcc tggatcgagg
     8341 cgttgaagtc tgtcgtgagc ttctcgatag ccttggcctg cgcctcgacc agaggcccga
     8401 ccgcgaggcc cagatcttcg gcacgacgct tggcgtccgc gaactgcttg ttcagttcct
     8461 tgatggcctg ctcggcctgc gtcacgggct tcgcgatgcc ggtgagctgg tcgtacaatt
     8521 gggcgaacgc gatgtcctcg tttagctgtt cgaggctgcg aacgctgccc ttcgtgacct
     8581 tgccctggtt gaggacggtc aggacgctat cggagacgcc cgtcaggttt ccggcgcgtg
     8641 cggcctcctg gagaatgccg gagatcacca gcccggcgat tgcctgcggg tcgtctccag
     8701 cgccgagcga gatccccgag ccataggaac cgagcccggc gaaatagcgc gtgcccttac
     8761 ccgtctgctt gaccttgacg tagccgccgg tcgcgccctc caaaatcgag ccgccgagat
     8821 ccgccacgat ctggttgacc gcatcggcca cgctctggcc gagcgtccgc gtgccggcga
     8881 ccatcgagcc gccgttgttg gccgaccacg gcgcacccgg aaccaactgc ccgccctgga
     8941 acagcagatt ttgcgtgacc tgcggaatcc ctttcttgcc gccgaacagg tcgccgacga
     9001 ggttgcccaa aatcccgcca atggcaccgc cgataggccc gccaatggca gtgcccagca
     9061 gcgcaccgcc gagcccgagg ccagcctgct tgacgttgcc gctgatcaag cccggcagca
     9121 gcgcgcccag gccgggcaaa aacgcctcag ccaggaacgt gctgccgaga aacgtgccgc
     9181 cggacccgag cagccctgcg gtcgcggcga tcggatgcga gccgcctacg gccggcaggc
     9241 cgaacagcca ggcattgagc ccgctgaacc cgccggccag cgcccggccg atgccgccgg
     9301 cggcgtcgat gatcgagccg gtcccgatac ccacgccacc gggcagcccg ccgccggacg
     9361 tgggcatgag gcccatcccg cccgcgatgc cgcccaggcc gacgccgccc aggatgccag
     9421 ccaccacagg gcggaacacc agaagcgccg cgatctccgc ggcgagccgg gtgaagatgg
     9481 atttgatccc gctggcgaga tcgcggaacg aatcgatccc gccgtcgaac accgactgga
     9541 agaaatcggt gaaactgtcc tggatgcctt cgagcgcgtt ctcgaacggc cggcgcatca
     9601 actcagcttc ctgctccgcc agccgctcgc gctcgcgccg ctgctcctcg taggctcggt
     9661 tcgccgcctg cactgcagga ttggcctcgc gcatggcgcg gttgagcgcc tgttgcgctt
     9721 cgatcgacag cgtgtagccg cgctccgccg ccttttccaa ggcgcggttc aggtcatcga
     9781 tctgcttctg gtagcgctgc tgatcggtga gcccggactc gacggtgcgc tctatcaggc
     9841 ggaccgcgtc ggcatactcc ctggcctcgt tcgcgatcgc cttctgattg agatcggcga
     9901 ggatgtcgcc cagcgtccgc accttgggcg atgcccggtc ggccgcatcg ccgagatcct
     9961 tggtcgcctt cacggtgcgc tgcattccag gcaccgtgcc gcggctgatc tggtcgcccc
    10021 acagcagcac ctcgagatcg cggatctcgc gcttgagctg gtcgatccgc gcgttggacg
    10081 gccgcccctc cgtgacgacg gacacctcct gcccggcgat ctcgtcgccg tatgagccga
    10141 actgcccgcg ctcggcgcgc cgccgctcct cctccagccg ctccaggtcg cgcagctcct
    10201 ggcgcatcag cgcgagccgg ttctgtccct cgatccgccg cgcgttgctg aggttgaccg
    10261 ttgaggctgt cagctcctcg gtgagctgct tggcgcgctc catcagctcg ttgtggcgct
    10321 cctgcgccga cgtcgccgcg tcggtcgcgc cctcaaggtc caggaaggac atggccagcg
    10381 cgccgaccac ggcaccggcc gcgccaatga tcgcgcccca ggggccgaac atcgaaatga
    10441 gctgggtacc ctgctgcacg aacgcgcgca gcggattggt gcccgacgcg atctggaccg
    10501 cgaagtcgcc gacctggaag cccgcctgct gaatggcgga gccgaaccgg cggttcgtgt
    10561 tcgcggccac gccgatctgc cgatcgagca agttggccgc ctgcccggcc cgcgacatac
    10621 cctcgatcat gcggaggctg gcgttgttca cggccgacat gccggcgatc atgcgagcac
    10681 tggcggaggc gttcgcggca gcgagagcct gaaggccgga ttgcgtgcgc tgccgctgcc
    10741 ggtcgatccg ctcaagcgcg ctcgtgacct ggctggagcc ctgctgcgct ccgctcgcgt
    10801 caacgacgat gcggacgact cttgtcgtgt ctgccattgt cagattccgc cttgtcgtcg
    10861 tgcctcgcct gcatctccag ccacgcccga tccatcgccc ggatcaccgc gaccagcgcg
    10921 tcgaactcgt cgctctcgat gccgtgccgc cgtgcccatt ggtcgattgc cgaccacggg
    10981 atctccagcg gtccgccgaa tgaatagggc cggcacgtgt gcagatcgtg gaaggcgtcc
    11041 caataccagc ggaggccgtc cagggacggg cggcgctcca gggcggggat acgctcccct
    11101 tccgccgcaa gagcctcgaa ccattcagcc cggctgccga actcgcgttc ccaccgcagc
    11161 cgggccgtca gtttcccgcc gcatcctcga tctgtgcggc gcggaagttt gcgacccgcc
    11221 cggcctggtt ctggatgtcc gcgaacaggt cggggagatc gaggagcagc cggatcacgt
    11281 tctcccggct gaactcgata gcctcgccgt cgcgcccgat catcttgccg tcgccgtgct
    11341 ctttcgatcc ccatgccaga atgacggcct cggcatacgc ttcggccatc agcctgtttg
    11401 caagctcgtc ctccatcgtg ccggcctgca actgccggcg atagggctgg agcttcgcgt
    11461 ccaggacgcg cgcgaacttc ttgttcgcgc cccccgcccg cgcgatgcga agccagaacg
    11521 cgccgtaatc aaggatgatg ccgtccttct cggccttcgc atccgtgccg aacagctcgt
    11581 agggggaagc cattacgcga cctcgcgggc gatcttcagg gtgcagcttt cggacgagtc
    11641 gtagaggccc tgccagccgg cattgaccac gacat
//
LOCUS       DASVDU010000214         3033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_988466, whole genome shotgun sequence.
ACCESSION   DASVDU010000214 DASVDU010000000
VERSION     DASVDU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988466"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VF226_13695"
     CDS             complement(<1..549)
                     /locus_tag="VF226_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7075090.1"
                     /translation="MSDQDIAPEMKIMPKVGSSPDDRMPPLSPEQLTDEQRAVAEEFK
                     EKRGFEIGGPFLTLIRSPEIFKAADHLGRYLRFGSPIPSKLKELAILITARRWTQQYE
                     WYTHRQVAMHAGLAPEIADAIAEGRRPDNLAEDEDAVYRFCTEVHANGAVSDATYGRV
                     VALFGEQGVIDLCAICGHYSTLA"
     gene            complement(590..1405)
                     /locus_tag="VF226_13700"
     CDS             complement(590..1405)
                     /locus_tag="VF226_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075091.1"
                     /translation="MRRLEDKVALVVGAGSVGPGWGNGRAISVLFAREGAKVFGADKN
                     PDAAAETQRLIAEEGGSATVGEVDVTDPGSIAAMVDACIQTYGRIDVLVNNVGGSAPG
                     DVVSMPLEVWDRQFDTNIRYVFLTCKHVIPRMVAQGGGAIVNLASIAALAHFGPEVVA
                     YSAAKAGLIHFTRVTAVRYARDHVRCNIVVPGLMDTPLVEQRLAGERGGGDVEALRRR
                     RNAQPPMGRMGTAWDVAHAALFLASDEARYITGAELVVDGGLAATVRLAGDAQ"
     gene            complement(1567..2283)
                     /locus_tag="VF226_13705"
     CDS             complement(1567..2283)
                     /locus_tag="VF226_13705"
                     /inference="COORDINATES: protein motif:HMM:NF021376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HEX7075092.1"
                     /translation="MTPDEKQLISGLFDRMRSFGQPQKDPEAEALIRDSVQQIPDAAY
                     MLVQSVLVQEHALQQADQRIRELEAKVNELERQAAEAPAQTGGFLGGLFGGGARPAAP
                     AQRATGGSVPSAGSRLGAGPSGRSGPIGAPTRPLGQQPQGQQAGGGFLQSAMATAAGV
                     AGGMLLANTISSMLGGGSSAQASTASSGNEAAHQPEHNHAQDAAADNSANDADADFQD
                     ADYEPDAGDWGGGDWGDFEI"
     gene            complement(2365..>3033)
                     /locus_tag="VF226_13710"
     CDS             complement(2365..>3033)
                     /locus_tag="VF226_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX7075093.1"
                     /translation="AAELAGAIAASPHLSFAGLQAYYGAAQHFRTEKERTAAIASAAE
                     RVQDTLAELKKYDLTCRIIGGAGTGTFEHEAASGIWNELQPGSYIFMDADYARNQKAD
                     GSRFDTFEHALFVLATVMSTPAPERAVVDAGHKALSNDSGFPEIPSLPGAKYHRPSDE
                     HGVLDLTACDRRPQLGDKVLLIPGHCDPTVNLHDWYVGVRGLHTPDAHVEAVWPVAAR
                     GAIF"
BASE COUNT          478 a         1006 c          998 g          551 t
ORIGIN      
        1 cgcgagcgtg gagtagtggc cgcagatcgc gcaaaggtcg atcacgccct gctcgccgaa
       61 gagcgccaca acacgtccgt aggtcgcatc actgacggca ccgttggcgt gcacctcggt
      121 gcagaatcga taaaccgcgt cctcgtcttc cgcgaggttg tccggacggc gcccttccgc
      181 aatggcatcg gcgatctcgg gtgcgagccc tgcatgcatc gcgacctggc ggtgcgtgta
      241 ccattcgtac tgctgcgtcc agcgccgggc cgtgatgagg attgcgagct ccttcagctt
      301 ggatggtatc gggctgccga agcgtagata ccggccaaga tggtcggcgg ccttgaagat
      361 ctccgggctg cggatgagcg tcagaaacgg gccaccgatc tcgaacccgc gcttctcctt
      421 gaactcttcc gcaaccgcgc gctgttcgtc cgtcaactgc tcgggagaaa gcggcggcat
      481 gcgatcgtcc ggtgagctgc ccactttcgg catgatcttc atctcgggcg caatgtcttg
      541 atcactcatt ccctggtccg cctttctcac gctcgatcga tttaaggagc tattgcgcgt
      601 cgcccgcgag ccgcaccgtt gcggcgagcc cgccgtcgac caccagctcc gcgccggtga
      661 tgtaacgcgc ctcgtcggag gcaaggaaga gggctgcgtg tgcgacatcc caggccgtgc
      721 ccatgcgccc catcggtggc tgcgcgttgc gccgccgccg cagcgcctcg acgtcgccgc
      781 caccgcgctc gcccgcgagc cgttgctcca caagcggcgt atccatcaga cctggcacga
      841 cgatgttgca gcggacgtgg tcgcgtgcgt agcgcacggc ggtcacgcgg gtgaaatgga
      901 tgagcccggc ctttgccgcc gagtaggcga ccacctcggg tccgaaatgg gccagcgccg
      961 cgatggaggc gagattgacg atggcgccgc caccctgagc caccatgcgc gggatgacgt
     1021 gcttgcaggt gagaaagacg tagcggatgt tggtgtcgaa ctgccggtcc cagacctcga
     1081 gcggcatgct gacgacatcg ccgggggcgg acccgccgac gttgttcacc agcacgtcga
     1141 tccggccgta ggtctggatg caggcatcga ccatggctgc aatggagccg ggatcggtga
     1201 cgtcgacctc cccgaccgtc gcggagccgc cttcttccgc gatgaggcgc tgtgtctctg
     1261 cggctgcatc cggattcttg tcggcgccga agaccttcgc gccctcgcgc gcgaacagga
     1321 cggaaattgc gcggccgttg ccccaccctg gccccaccga gcccgcgccg acgaccagcg
     1381 cgaccttgtc ttcgagacgg cgcatttgca tttcccccag gttcataccg ggcgcatcct
     1441 agtgcggagc cacagccttt ggccagcatc cctcgcggtc cgcggcgaag cgttggcaca
     1501 caaaaaattg cgggctcccc gagaagagcc cgcatctgat catctcaacc tgagagttgt
     1561 cgatttttag atttcgaagt cgccccaatc gccgccgccc cagtcgccgg catccggctc
     1621 gtagtcggca tcctggaaat cggcgtcggc atcgttcgcg ctattatcgg ccgccgcgtc
     1681 ctgagcgtgg ttgtgctccg gttgatgggc ggcctcatta ccggacgagg cggtcgatgc
     1741 ctgtgccgat gagccgccgc cgagcatgct cgaaatggtg ttggcgagca gcatgccgcc
     1801 tgccacgccc gccgcagtcg ccatcgcgga ctgcaggaat ccgccgcccg cctgctgccc
     1861 ctggggctgc tggccgagag ggcgggtcgg agcgccgatg ggtccgcttc tgccggacgg
     1921 gcctgcgccg agccggctgc ccgcactggg cacgcttccg cctgttgcgc gctgggccgg
     1981 ggctgcggga cgggcgccgc caccgaacag accgccaagg aagccaccgg tttgcgccgg
     2041 tgcttcggcc gcttgccgct cgagctcgtt gaccttcgcc tcgagctcac gaatgcgctg
     2101 gtcggcctgt tgcagggcat gctcctgaac gagcaccgat tgcacgagca tgtaggctgc
     2161 atccgggatc tgctgcaccg agtctctgat cagagcctcg gcttccggat ccttctgcgg
     2221 ctggccgaac gagcgcatgc ggtcgaacag accggaaatg agctgcttct cgtctggggt
     2281 catcgcggtc tcctggcgat tggtcacggc cgtcatgtaa gccttggcgg acgagctgca
     2341 agaggcgcct cgtgcctatt tgggtcagaa gatcgccccc cgggccgcca ccggccagac
     2401 tgcctcgaca tgggcatctg gcgtatgcaa gccgcggacg ccgacgtacc aatcgtgcag
     2461 gttgaccgtc gggtcgcagt ggccggggat cagcagcacc ttgtcgccga gctgtggacg
     2521 gcggtcacat gcggtgagat cgaggacgcc gtgctcgtcc gatggccgat ggtatttcgc
     2581 gccggggagt gacgggattt ccggaaagcc tgagtcgttg ctcagcgcct tgtgccccgc
     2641 atccacgacg gcacgctccg gcgccggggt gctcatgacc gtcgccaaca cgaagagggc
     2701 gtgctcgaac gtatcgaaac ggctgccgtc cgccttttga ttgcgggcgt aatccgcgtc
     2761 catgaaaatg taggagcccg gctgtaactc gttccagatg ccgctcgcgg cttcgtgctc
     2821 gaacgtaccg gtgccggcgc cgccgatgat gcggcaggtc aggtcatatt tcttcaactc
     2881 ggcgagcgtg tcctgcacgc gctcggccgc actggcaatt gctgccgtgc gctccttctc
     2941 cgtccggaaa tgctgggccg ctccgtaata ggcttgcagg ccggcaaagc tgagatgcgg
     3001 agaagcagca atggcgccgg cgagctcggc ggc
//
LOCUS       DASVDU010000215        17388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_36523, whole genome shotgun sequence.
ACCESSION   DASVDU010000215 DASVDU010000000
VERSION     DASVDU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17388
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36523"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VF226_13715"
     CDS             <1..175
                     /locus_tag="VF226_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX7075094.1"
                     /translation="HRFNPGGGYSNFMMDDEGEARVRATYGENYTRLAMLKKKYDPTN
                     LFRVNQNIRPAVQ"
     gene            complement(1193..2605)
                     /locus_tag="VF226_13720"
     CDS             complement(1193..2605)
                     /locus_tag="VF226_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX7075095.1"
                     /translation="MTSAIEITSGDAAYRGIRTAQGDRFLGIRYAEPPVGNLRFKPPV
                     PVRKKGLIDATRPGFSPPQVRRPLPAWAPQGEGLETGEDCLNLNVFTPAADGARRPVI
                     VHAFGGGFQTGSAFGTFHDEAGFTARGNVVLVRPNMRVGALGFLHLGEAFGADFAAAN
                     RGMLDFVAALQWVHDNITAFGGDPDNVTLVGMSSGAFTISALFGVDGIDHLYCRVWVM
                     SGPTSRIIAADTAAVIAADFLERADIEPGDVRALEALPVERILELQEQVLATHLGERN
                     APGGRTLGIVLDGVSLQRHPLDGLASGSFASHQIVAGWTRDEARMWYAAGIMPEVPTR
                     EALLASIARFHPDDAEERLSALEAELPGLSFTQYEEIFLSRAIYRDPSLRMVETHIAA
                     GGRAYAYQFEWVPPFEGGRLGAAHGFDEPFVFNSLDRVPLTVGVPGAKQLAEEMSRAL
                     IGFASTGGISGWEAPWHVFR"
     gene            complement(2602..3546)
                     /locus_tag="VF226_13725"
     CDS             complement(2602..3546)
                     /locus_tag="VF226_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075096.1"
                     /translation="MTDDTILSVSGLSKNFKAVKAVRDVSFSIRRGKTFGLVGESGSG
                     KSTTARLVLRLIEPTSGSIRFRGRELANFSPAELRAERRHMQMIFQDPYGSLNPRMSV
                     RETLIEPQLVHGIGTPAERIQTAELLLAEVSLPRAALDRHPHEFSGGQRQRIAIARAL
                     ATNPELIVADEPVSALDVSVQAQVLNLMRDLQDRHRTTMLFISHDLRVVQFMCDEIAV
                     MYLGEVVEQGPRDRIYYSAAHPYTQALLASATGKRDGKRARLKGEIPSAHAVPSGCAF
                     RTRCPHAFERCALEKPAIRDLGLGQKAACHLLDGQAVS"
     gene            complement(3543..4562)
                     /locus_tag="VF226_13730"
     CDS             complement(3543..4562)
                     /locus_tag="VF226_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075097.1"
                     /translation="MTVRTASDSTPTAQPASARELVLEVRDLSVSLRNADGLLPIVTD
                     VSFSIAKGEAVALAGESGCGKSITASAIMGIAPDNAEISGTIILNGAAISGLPHRERR
                     KLCGRHMGFIFQEPMTALHPTLTVGRQMTDTLRHNLGLSHAAATDRAAELLDRVGIPR
                     AREILKGYVHELSGGMRQRVMIALAISCGPSLVIADEPTTALDVTIQAQVLDLLDEMR
                     REFELAMLFITHDLELVGDFCDRAVVMYAGDVVERGRAADIVRSPAHPYARGLIDAMP
                     RHGDQRARLTPIPGTVPPVGHWPQGCRFAPRCSRAGKKCTVRPPLAVHIRSDVRCWYP
                     LKVGQ"
     gene            complement(4559..5503)
                     /locus_tag="VF226_13735"
     CDS             complement(4559..5503)
                     /locus_tag="VF226_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075098.1"
                     /translation="MLRYLLKRFLLILPTIFVPLIIVFLLLRLSPGDPAGMMLGDQAT
                     PEQIEALRTQLGLNEPIWVQFAIWLKGILTLDLGNSIFFREPVMEVIPRYVSVTVLLT
                     IVALILAVAIGITMGLISAMHRNKPIDRGVVAMAVLGISLPEFWFALLLIFVFAVTLR
                     WFPVAGYNPPSAGLIAFGATILLPAIALGVRQSALMTRMMRSSMLDVLNEPYITTARA
                     QGLPERKVIGSYAMRTAAIPVVTVAGLAAGYMLGGAVAIEIIFALPGLGRMLVEAVSR
                     RDYPLVEGGVLTISLILAVLNLVIDVIYAILDPRIRYQ"
     gene            complement(5505..6407)
                     /locus_tag="VF226_13740"
     CDS             complement(5505..6407)
                     /locus_tag="VF226_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075099.1"
                     /translation="MSTTQATHNERLTDGGEEPFATTTKSVWQRLLANRTAIVSIAIL
                     VIISLAAILAPVMAPGNPLRVSPANRFIPPSAEHIFGTDHLGRDMFKMVVHGARTSLL
                     VGLAVTVISMSVAVVLGAISGFYRQVDVVLMRLVDGLMAFPGIVLATAAAGIMGPSTR
                     TVIISLSIVLIAPSLRVVRGQVLVVRELQMIEAARAVGVPTLRLFTRYILPAVSSPIL
                     VQASFIFSAAVLGEAGLSFIGVGIGPKELSWGNALTEARNYISQAPWIVLFPGLALML
                     TILALNLLGDSLRDILDPRLTRRR"
     gene            complement(6411..7982)
                     /locus_tag="VF226_13745"
     CDS             complement(6411..7982)
                     /locus_tag="VF226_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075100.1"
                     /translation="MLRKITTRSLLLFGVSLAALSAQSSMAQEFQRGGTLRVGISQMA
                     PSPDPVVTTFGVNWMTATVACEGLFGTDETWTPQPVLAESYEYSDDGKTLTVTLRKGL
                     EFHSGAALTSADVVTSLNRFREAAGIGAALKNIVTEIAAPDDHTVVFHLASPSPIVPG
                     LLSGAQASIMSRASLEGASPTEATRGLDCTGPYVLTEYKPDQGAILTRWEDYQSLSTP
                     SSAFTGAKHAYADQIHLRLMPEASVRRDSLITGEIDIALELPTDFYDALQNDPNTEAV
                     VVPNNQSLTVVFNTKEGVASDVNLRRAIYYALDMESIMLASVGNPNFFTLDPSWIPDP
                     NSFWYTTAGVPEGFGKPQPEKVKEYLAASNYKGEPIRWLVASEQYQKHYLPAVTAAQQ
                     LEEYGINVEIVESPMANYIQARADPAQMDAFSSFLPTYVDPTSIAYLNATYPGFWTDP
                     EKMDLMNKLATTIDVNERKAIFEKVHALAYDQFPWIKYGTEANMYGIRKGVGNPARVP
                     SRGYDFYNVAPPSKN"
     gene            complement(8002..8373)
                     /locus_tag="VF226_13750"
     CDS             complement(8002..8373)
                     /locus_tag="VF226_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075101.1"
                     /translation="MTRIVKLHEIAHGRSGDKGNRLNVSIIAYEERHWPILLKQVTAE
                     RVANLFAYRGVTEVRRYELPHLQALNFVIEDALEGGVNSSLAIDTHGKCLSYHVLGME
                     VLVEDEMAEGASPNADAQGVT"
     gene            complement(8379..9767)
                     /locus_tag="VF226_13755"
     CDS             complement(8379..9767)
                     /locus_tag="VF226_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX7075102.1"
                     /translation="MIQTTKEGALYRIGCGAGFSGDRIDAPQAVVAAIAAGGGGAIIF
                     ETLGERTLALGHIARRQDPSKGYEPRLKDLLEPILHVCISAGITIVGNFGQANPPAAA
                     ALIQQIADRLALGTLRIAIVSGDDLTDKVDLAVTERWEGDRRLPPIDGEIIAINAYIG
                     ALPIAEAIKVGAQVVVTGRVADPALALGPLLAHFGWDWTDLDRLAAGTLVGHLLECGS
                     QVTGGFFADPGFKDVPDMADIGFPIAEVEADGSFVITKPEGTGGVVDLRTVKEQLLYE
                     IHDPAAYLTPDVTLDITGVQLAQVGKDRVRVTGARGRPAPNTLKATIGYYGDWLGEAE
                     ISYAGPNALARAHLAVETITARLARRGLTLRHRADIIGAVSAFDNEGGHLSLGYTDAG
                     SGDYRVRFAFGSACKRDVEHAVQEVNALYCCGPAGGGGVRQSVRPRVRTVSHLVPRHL
                     VRPAFHFFGEES"
     gene            9855..10532
                     /locus_tag="VF226_13760"
     CDS             9855..10532
                     /locus_tag="VF226_13760"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7075103.1"
                     /translation="MNSLAKVRSDQTKSWDAVCRELQRQIIAGQLRPRERLVEDEIMA
                     RTGATRHAVRRAFDELERMGLVVRQPNKGVQVRDYSIVEIEELYEIRECLELRAAARF
                     NKPADQPLVDQLTEIATRHRDASRAQRFGEVFALNNQFHETMYRAAGNKLLADAIMHY
                     TFATHPIRTRAFPNDELREIAITEHFAMIDAIARGDGKGLGEIIRAHITQPKEFYLRA
                     TYIQDIL"
     gene            11210..11504
                     /locus_tag="VF226_13765"
                     /pseudo
     CDS             11210..11504
                     /locus_tag="VF226_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043766018.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine zipper domain-containing protein"
     gene            complement(11511..11852)
                     /locus_tag="VF226_13770"
     CDS             complement(11511..11852)
                     /locus_tag="VF226_13770"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEX7075104.1"
                     /translation="MERRASIELRAKGRRLEGYTALYGVDTRISDFTEVILPGAFTET
                     LRQGRDILALADHDPKAVLAHEKRHAPPLRRQQGSRFHLDVPNTTAGRDVLELAERGD
                     IGGMSFGFTVG"
     gene            complement(12270..12449)
                     /locus_tag="VF226_13775"
                     /pseudo
     CDS             complement(12270..12449)
                     /locus_tag="VF226_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024975.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(12915..13574)
                     /locus_tag="VF226_13780"
     CDS             complement(12915..13574)
                     /locus_tag="VF226_13780"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7075105.1"
                     /translation="MSLRINDTAPDFTAETTEGTIRFHDWIGDGWAVLFSHPKDFTPI
                     CTTELGTMAGLKGEFDKRNAKIIGISVDPVESHDKWKGDIEKATGHTVNYPMIGDKDL
                     KVAKLYDMLPASAGDSSEGRTPADNQTVRSVFVIGPDKKIKLILTYPMTTGRNFQEIL
                     RALDSLQLTAKFPVSTPADWQPGEDIVIAGSVSDEDAVKYFGAFEAVLPYLRKAKQPS
                     D"
     gene            complement(13737..14597)
                     /locus_tag="VF226_13785"
     CDS             complement(13737..14597)
                     /locus_tag="VF226_13785"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7075106.1"
                     /translation="MDARILAALKYVEQNINEQIELEEMAARVGLSKFYFERLFQTEV
                     GESFYAYFKRVKMHNAACRLKWTNQSIYEIAIGYGYSSNAAFTRAFRAFFGVSPTEYR
                     SSDEWDPKIFYKDRFDGLSFDDPPKVQVRDIGSYRCLFRRYYGPYETVQDSWRDFIER
                     LPDALQGTEPGRARFLGRVYDDPRVTPPNEIRYDCCYVFADAEDARLQLDEVKDCLVT
                     TDAGLYAVVDNNREPRPRPEVYAYILDKWMPRTNYQYSDVPALEFFTKSPVEANSTWA
                     PACTMLVPLE"
     gene            complement(15395..16261)
                     /locus_tag="VF226_13790"
     CDS             complement(15395..16261)
                     /locus_tag="VF226_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7075107.1"
                     /translation="MSIEPPEVTAFYDRPTGSIQYVVADPITRRCAIIDPVLDFDERS
                     GRVSTANADLLLDFIERQQLTLEWILDTHPHADHFSAAAYLKKKTGIATAIGKHVVDV
                     QELWKAIYNWPDFRADGSQWDVLFDDGDTFWIGMMPVKVLFSPGHTLASVTYVVGDCA
                     FVHDTIFMPDSGTARCDFPGGDAAVLWNSIQKILALPDDTRIFVGHDYCPGGRDPKWE
                     SSVAEQKAENIHISHYRTKAEFVAMREARDRTLPMPRLILHALQVNINGGQLPLPEAN
                     GRRYLKIPLDSL"
     gene            complement(16621..17160)
                     /locus_tag="VF226_13795"
     CDS             complement(16621..17160)
                     /locus_tag="VF226_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX7075108.1"
                     /translation="MKKAALVLRLFVSSICLMPLDAALAQEGVEMQSPTSLSETYEAW
                     TVRCVNQQQGEKTQRVCQMAQTLLQQGSQQRVLTFALRRRDDTARATLVFPFGLLLSE
                     GFRVEIAEEEVLRGTYRTCLPTGCIAEVEVPAETIEKLEAAETASVLMTATTGQQIRA
                     DISLKGFKAAYQRLVALAA"
BASE COUNT         3424 a         5094 c         5532 g         3338 t
ORIGIN      
        1 gcatcgcttc aaccccggcg gaggatattc caatttcatg atggacgacg agggcgaggc
       61 gcgtgtgagg gcgacctatg gcgaaaacta cactcgcctt gccatgctca agaagaaata
      121 cgatccgacc aacttgttca gggtgaatca aaacatccgg ccggcggtcc agtgaccggg
      181 ccggagtccg ccgaatcgaa aaccctctga catttggcgc tgcaggcccg tccggatgcg
      241 tgccgtcgta tcggctagtt tgcgggagag ggtcaactat cagacttgtc ggcgaggcgc
      301 tgcgggtcgc tgtcaaggag cagcagactg gcgcgcctcc cggccggcgc gccgccgacc
      361 agagagagcg agagcgaagc ggcagcgccc cgccttcccg accgacctgc cagtagggtc
      421 cgctgagggg ctgcatgaag cgaggctcgg cgggcggagt cgcaacgttc agcgccccgc
      481 tcgcgctgac cgtcaggacg ctgagggatg gagaccgttg cgtgcccgct atgtaggcag
      541 gggaaagcag tcgaggtttt ccaacgctgc acggcccgga tgaagaaacc gggcgaatcg
      601 cacctgcagg tagtagcgtc cctcacgccg gacatgtagg caggcagcgc catggcatct
      661 ccgcgtatat ctgcgtgaga tgcctcacag tcgtccgaac ctccgtccgt tgctaactgc
      721 ctgattttta tgggctttca aacaacggta gcggaggagg gacccgaacc tctaaaggcc
      781 gctaggtcat tattgaaagt ccataaaaac tgaactctct cacgcgtgga cctgtatatg
      841 tgcgtatcta gatcccggac gggcgtcaag gatccaacga tcgctgagcc taagccgggg
      901 ttcgaattcg gcgctaccgg atttctcaca gcccggggta caatgaggta ccagcttcgg
      961 ccgctttggt cctagcacag acggcgcggc ggaacgaagt tggcaatcac gcgagcccgg
     1021 ccagtctggg aactgccgcc atcgtcgtcc tcagggcaag ccgcaggaat tcttttcgtc
     1081 gggtcggcgc gagatcgtcg tcatgggcac tctcgctatc ggacaggccg tcgctggatc
     1141 gttggagccg cgaggaacgt cgcaagctga gccttaaggg gtattgctct cctcagcgga
     1201 acacatgcca gggcgcctcc cagcccgaga tcccgcccgt ggacgcgaag ccgatcagcg
     1261 cacgcgacat ctcctccgcg agctgcttgg cgccggggac tccaacagtt aggggcaccc
     1321 tgtccagact gttgaaaaca aagggctcgt cgaaaccgtg cgccgcgccg agacgcccgc
     1381 cttcaaatgg aggtacccat tcgaactgat aggcataggc ccgcccgcca gcggcaatgt
     1441 gcgtctccac catgcggagc gacgggtcac gatatatggc ccgcgaaagg aagatctcct
     1501 cgtattgggt gaagctcagg cccggcaact cggcctcaag cgcgctaagc ctctcctcgg
     1561 cgtcgtcggg gtgaaaccgg gcgatggaag caaggagagc ttcgcgcgtc ggcacctccg
     1621 gcatgatccc ggctgcgtac cacatccttg cctcgtcgcg cgtccagccg gcgacgatct
     1681 gatggctcgc gaagcttccg gaagccaagc cgtccaatgg atggcgctgg agactaacac
     1741 cgtcaaggac gatgcctaag gtgcgcccgc ccggcgcgtt tcgttcgccg aggtgggtgg
     1801 caagaacttg ctcctgcagt tcgagtattc tctcgaccgg cagggcctcc agcgcccgta
     1861 catcacccgg ctcgatgtct gcgcgctcca gaaagtccgc ggcaatgaca gctgctgtat
     1921 ctgccgcgat tatgcggctc gtcggtccgc tcatcaccca gacgcggcaa tagaggtggt
     1981 cgatgccatc gaccccgaac agggctgaga tggtgaaggc gcccgaggac atgccgacaa
     2041 gggtcacgtt gtcggggtcg ccgccgaagg cagtgatgtt gtcatgcacc cattggagag
     2101 ccgcgacgaa gtcgagcatg ccgcggttgg ctgccgcgaa gtcggcgcca aaggcctcgc
     2161 ccagatgaag gaagcccaag gcgccgacgc gcatgttggg gcgcaccaga accacattgc
     2221 ccctggcagt gaagcccgcc tcgtcgtgga atgtcccaaa ggctgagccg gtctggaaac
     2281 cgccgccaaa tgcgtggacg atcaccgggc ggcgcgcgcc gtcggctgca ggcgtaaaga
     2341 cgttgaggtt gaggcagtcc tcgccggtct ccaagccttc tccttgaggc gcccaggcgg
     2401 gcagggggcg acgcacctgc ggaggagaaa acccgggccg cgtggcgtcg atcagccctt
     2461 ttttcctgac cggcaccggc ggcttgaacc gcaggtttcc gacaggcggc tctgcatagc
     2521 ggatgccgag gaaccggtcg ccttgagccg tgcgaatgcc gcgataggcg gcatcgccgc
     2581 tggtgatttc gattgcggac gtcatgacac tgcctgtccg tcgagcagat ggcaggccgc
     2641 cttctggccc aacccgaggt ctcggattgc cggcttttcg agcgcacagc gttcgaacgc
     2701 atggggacag cgtgtgcgga aggcacagcc gctcggaacc gcatgggcag agggaatttc
     2761 gcccttcagc ctggcgcgct ttccgtcgcg cttgcccgtc gcggacgcca gaagcgcctg
     2821 ggtataagga tgcgccgcgc tgtagtagat ccggtcgcgt ggcccctgtt cgacgacctc
     2881 tccgagatac atcacggcga tctcgtcgca catgaactgg acgacgcgca ggtcatgcga
     2941 gataaacagc atcgtcgttc tatgtcggtc ctgcaggtcg cgcatgagat tgaggacctg
     3001 cgcctgcacg gacacgtcga gggcgctcac gggttcgtcg gcgacgataa gttcgggatt
     3061 ggtagctagc gcccgcgcaa ttgcgatgcg ctgccgctga ccgccggaga actcgtgagg
     3121 gtggcggtca agcgccgccc gcggcaagct gacttcggcg agcaggagct cggccgtctg
     3181 tatccgttcc gcaggcgtgc cgatgccatg aaccagctgc ggctcgatga gcgtttcgcg
     3241 cacgctcatg cgcggattga gcgagccgta agggtcctgg aagatcatct gcatgtgacg
     3301 ccgctccgcg cgcagctcgg ctggggagaa attggcgagt tccctgccgc ggaaacggat
     3361 ggagccgctg gtgggctcta tgagcctgag aacaagacgt gccgtcgtcg acttgcccga
     3421 gccggactcg ccaacaagtc cgaacgtctt tccgcgccgg atggaaaagc tgacatcccg
     3481 gactgccttc accgccttga agttcttgga gaggccggag acgctcagaa tggtgtcgtc
     3541 ggtcattggc cgaccttcag tgggtaccag cagcggacgt cggaacggat gtggaccgca
     3601 aggggtggcc gaaccgtgca tttcttaccg gcgcggctac agcgcggggc gaagcggcat
     3661 ccctgaggcc agtggcctac aggaggcact gtgccgggaa tgggggtgag gcgggcgcgt
     3721 tggtcgccat gcctcggcat ggcatctatg aggccacgcg cataagggtg ggccgggctg
     3781 cgcacgatgt ctgcggcgcg tccgcgttcc accacatcac cggcatacat gaccacggcg
     3841 cggtcgcaga aatcgcccac caattccagg tcatgggtga tgaacagcat ggccagctcg
     3901 aactcgcgcc gcatctcatc caaaaggtcc agcacctggg cctgaatggt gacatcgagg
     3961 gcggtcgtcg gttcgtcggc gatgaccaga gatggaccgc atgaaatggc gagcgcgatc
     4021 atgacacgct ggcgcatgcc gccggaaagc tcatgcacgt agcccttcaa tatttctcgc
     4081 gctcgcggga tacccactcg atcaagcagc tcggcggcgc gatcggtggc ggcagcatgg
     4141 ctaaggccga gattgtgcct tagcgtgtcg gtcatctggc ggccgacggt gagcgtgggg
     4201 tggagcgcgg tcattggctc ttggaagata aagcccatat ggcggccgca gagcttgcgc
     4261 cgctccctat gcgggaggcc cgatatggca gctccgttta ggatgatggt cccgctgatc
     4321 tcggcattgt ccggcgcaat ccccatgatg gcgctggcag tgatcgactt gccgcagccg
     4381 ctctcgccag cgagcgcaac agcctcaccc tttgcgatcg agaacgagac gtccgtgacg
     4441 atcggcaaca ggccgtcggc attccgaagc gagacgctga gatccctgac ctcaagcacc
     4501 agctccctgg cgctggccgg ttgcgccgtg ggcgtcgagt ctgaagcggt gcgtaccgtc
     4561 attgatagcg aattcgcgga tcgaggatgg cgtagatgac gtcgatcacg aggttcagca
     4621 cggccaggat tagggaaata gtgagaaccc caccctcgac cagcgggtag tcacgccggc
     4681 tgacggcctc gacgagcatg cggccgaggc cgggaagggc aaagatgatc tcgatcgcca
     4741 ccgcaccgcc cagcatgtat ccggccgcca gacctgccac ggttacgacc ggtatggcgg
     4801 cggttctcat ggcgtaagag ccgatgacct tgcgttcggg caggccctgg gcccgggctg
     4861 tcgtgatgta gggctcgttg aggacgtcga gcatggagga tcgcatcatg cgcgtcatca
     4921 atgccgactg gcgcacaccc aaggcgatgg caggcagaag gatcgtggct ccgaaagcga
     4981 tgaggccggc actcggcgga ttgtaacccg cgacgggaaa ccagcgcagc gtgacagcga
     5041 agacgaagat cagcagcagg gcaaaccaga actctggcag ggaaataccc agcaccgcca
     5101 tggcgactac gccccgatcg atcggcttgt tgcgatgcat ggctgaaatc agccccatcg
     5161 tgatgccgat tgcgacggcc aggatcagcg caacgattgt caggagcacg gtgacgctga
     5221 catagcgcgg gatgacctcc atcaccggct cgcggaagaa gatcgagttg cccaggtcaa
     5281 gggtcaggat gcccttgagc cagatcgcga actgaaccca gatcggctcg ttgaggccga
     5341 gctgcgtacg cagggcttcg atctgctcgg gcgttgcctg gtctcccagc atcatgcccg
     5401 ccgggtcacc cggcgaaaga cgcaggagca ggaagacgat gatcagcgga acgaagatcg
     5461 tgggcagaat cagcaggaac cgtttgagga gatagcgcag catgtcagcg tctccgtgtc
     5521 agacgggggt cgaggatatc gcgcaggctg tcgcccagca ggttgagcgc cagaatggtg
     5581 agcattagcg cgaggccggg gaaaaggacg atccatgggg cctgggagat gtagtttctg
     5641 gcttctgtga gagcgttgcc ccagctaagt tccttggggc cgatgcccac gccaatgaag
     5701 ctcaggccgg cttcgcccag aacggcagcc gagaagatga atgaagcctg gacgagaatg
     5761 ggcgagctga cggcgggaag gatatagcgg gtaaagaggc gcagggtcgg aacgccgacc
     5821 gctcgggcgg cctcgatcat ctgcaactca cgcacgacca gcacctgccc gcgcacgaca
     5881 cgcagcgacg gggcaatgag gacgatcgac agcgagatga tgacggtcct cgtggacggg
     5941 cccatgatgc cggccgccgc agtagccaat acgatgcccg ggaaggccat gaggccatcg
     6001 accagccgca tcagcactac gtcaacctgg cgatagaaac cggatatggc gcccagaacg
     6061 acggccacgg acatggagat gaccgtaacg gcgagcccga ccaggagcga ggtgcgggca
     6121 ccatggacaa ccatcttgaa catgtcccgt cccagatggt cggtgccgaa gatgtgctcg
     6181 gcgctgggcg ggatgaaacg gttggcaggg ctcaccctca gaggattgcc cggagccatg
     6241 acgggcgcca ggatcgctgc aagtgagatt atgaccagga tggcgatgga gacaatcgcc
     6301 gtccggttgg ccagcagcct ctgccagacc gacttggtgg tggtggcgaa gggttcttcg
     6361 ccaccatcgg tcaggcgctc attgtgtgtc gcctgcgtcg tgctcatcga tcagttcttg
     6421 gacggcggag caacgttgta gaagtcgtag ccgcgcgagg gcacgcgagc cggattaccg
     6481 acccccttgc ggatgccata catgttcgcc tcggtgccgt acttgatcca ggggaactgg
     6541 tcgtaggcca gcgcatggac cttctcaaaa atcgccttgc gttcgttaac gtcgatggtc
     6601 gtggccagct tgttcattag atccatcttc tccgggtcgg tccagaagcc ggggtaggtg
     6661 gcattcaggt acgcgatgga agtcggatcg acgtaggtgg gcaagaagct cgagaacgca
     6721 tccatctggg cgggatcggc gcgggcctgg atgtagttgg ccatcggcga ctcgacgatc
     6781 tcgacattga tgccgtactc ttcgagctgc tgggctgcgg tgaccgccgg cagatagtgc
     6841 ttctgatact gctcggaggc gaccagccag cggatcggct cacccttgta gtttgaagcg
     6901 gcgagatatt ccttcacctt ttcaggctgg ggctttccga agccctcggg aacgccggcc
     6961 gtggtgtacc agaacgaatt ggggtccggg atccagctag gatcgagcgt aaagaagttc
     7021 ggattgccta ccgaggcgag catgatcgat tccatgtcga gcgcgtagta gatggctcga
     7081 cgcagattta cgtcagacgc aacaccttcc ttggtgttga acacgaccgt cagtgactgg
     7141 ttgttgggca ccaccacggc ttccgtgttc gggtcgttct gcaaggcgtc gtagaagtcc
     7201 gtcggcagct ccagggcgat gtcgatttcg ccggtgatca ggctgtcgcg gcggaccgac
     7261 gcttccggca tcagacggag gtggatttga tccgcataag cgtgcttggc acccgtgaag
     7321 gccgaggatg gagtgctcag ggactggtag tcctcccagc gcgtgaggat agcaccctga
     7381 tcaggcttgt actcggtcag cacatagggg ccggtgcaat cgaggccgcg tgtcgcctcg
     7441 gtcggtgagg cgccctcgag cgaagccctc gacatgattg atgcctgagc accggacagt
     7501 aggcccggaa cgatcgggga gggactcgcc aggtggaaca ccaccgtgtg gtcgtccggc
     7561 gcggcgattt ccgtcacaat gttcttgagg gctgcgccga tgccggcggc ttcgcggaag
     7621 cggttcagcg aagtgacaac atccgccgat gttagtgccg cgcccgaatg gaactcgaga
     7681 cccttgcgca gggtaacggt cagcgtcttc ccgtcgtcac tgtattcgta gctttcggca
     7741 aggaccggct ggggcgtcca ggtctcgtct gtcccgaaca ggccctcgca ggcgacggtt
     7801 gcggtcatcc agttgacgcc gaaggtcgtc accacaggat ccggcgacgg tgccatctgc
     7861 gagatgccga cgcgcaaggt gccgccacgc tggaattcct gggccatgga tgattgcgcc
     7921 gaaagggcgg caagcgacac cccgaataac agcagactgc gcgtagtgat ctttcgtaac
     7981 attttctcct cctttgtcct ctcaagttac tccttgcgcg tcggcgttgg gcgatgcgcc
     8041 ctctgccatc tcgtcctcca ccaggacttc catcccgagc acgtggtagc tcaggcactt
     8101 gccatgcgtg tcgatcgcaa ggctcgaatt gacgccccct tcgagagcat cctcgatcac
     8161 gaaattcagc gcctgcaggt gaggcagctc gtagcgccgc acctccgtaa caccgcggta
     8221 agcgaagaga ttggctacac gttcggcggt cacctgctta agcagtatcg gccaatgccg
     8281 ctcttcatag gcgatgatgg agacattgag ccggttaccc ttatcgccgg aacgcccgtg
     8341 ggcgatttca tgcagcttca cgatccgcgt catcccgctc acgattcctc accgaagaag
     8401 tgaaaggctg gacgcactaa atggcgcgga acgagatggg agaccgtgcg tactcttggc
     8461 ctcacgcttt ggcgcacgcc tcctccgccg gcaggaccgc agcagtagag ggcattcact
     8521 tcctggacgg cgtgctcgac gtcccgtttg caggcagagc caaatgcaaa tcgaacccga
     8581 tagtcacctg agccggcatc ggtgtagcca agggagagat gcccgccctc gttgtcaaag
     8641 gcgctgaccg caccgatgat gtcggcgcga tgtcgtaacg ttagccccct gcgggcgagg
     8701 cgcgcggtga ttgtctcgac ggcgagatgc gcacgagcca aggcgttagg gccggcgtac
     8761 gatatctcgg cttctcccaa ccagtcacca tagtagccga ttgtcgcttt gagggtgttg
     8821 ggcgccgggc ggccgcgcgc cccggtgacg cgcacccgat ccttcccgac ttgtgcgagc
     8881 tgcacgccgg taatgtccag agtgacatcg ggcgtgagat acgcagccgg atcgtgaatc
     8941 tcataaagca gctgttcctt gaccgtccgg agatcaacaa cgccgcctgt tccctccggc
     9001 ttggtgatca cgaagctgcc gtcggcctcc acctccgcaa tcggaaagcc gatatcggcc
     9061 atgtcgggca cgtccttgaa tccgggatcg gcgaagaagc cgcccgtcac ctgcgaaccg
     9121 cattcgagca ggtgtccaac aagggtgccg gctgcaaggc ggtcgaggtc ggtccagtcc
     9181 cagccgaaat gtgcgaggag cggcccgagg gccagggcgg ggtcggcaac gcgcccggtc
     9241 acgacaacct gtgcgcccac cttgatcgcc tcggcgatcg gcaaggcgcc gatgtaggcg
     9301 tttatggcaa tgatctcgcc gtcgatcggc ggcaagcggc ggtcaccttc ccatcgttcg
     9361 gtgaccgcaa gatcgacctt gtcggtcaag tcgtcgccgg aaacgatggc gatgcgcagc
     9421 gtcccaagag caaggcggtc ggcaatctgc tggatgagtg cggcagccgc cgggggattt
     9481 gcctggccga agttgccgac gatcgtgatg cccgcgctga tgcacacatg aaggatcggc
     9541 tcgaggaggt ctttgagccg aggctcatag cccttggacg ggtcctggcg ccgggcaatg
     9601 tggcccaagg caagcgttcg ctcgccaagg gtctcgaaga tgatggctcc gccgccgccg
     9661 gctgctatcg cagccacgac cgcctggggg gcgtcaatcc gatcacccga aaatccggcg
     9721 ccacaaccga tccggtacaa tgccccctcc ttcgtcgtct gtatcatcgg caggattatc
     9781 gataatgtca acgatgattg tgtgcggctg tgggttgcgt tactctttcg acgcggatcg
     9841 tgaggagctg agcattgaat tctctcgcaa aagtccggtc ggatcagacg aagagctggg
     9901 atgccgtttg ccgggagctg cagcggcaaa tcatcgcggg ccagttacgg ccgcgggagc
     9961 gactggttga agacgagatc atggcgcgca ccggcgcaac gcggcacgcc gtgcgcaggg
    10021 catttgacga gctggagcgc atggggctgg tggtacgcca gcccaacaag ggcgtgcagg
    10081 tccgcgacta ctccatcgtc gagatcgagg agctttacga gatccgggag tgcctggagc
    10141 ttcgggctgc ggcgcgcttc aacaagcccg ctgaccagcc gctggtggat cagttgactg
    10201 aaatcgccac caggcatcgc gacgcttcgc gcgcgcagag gtttggcgag gtctttgcac
    10261 tgaacaacca gttccacgag acgatgtatc gcgcagcggg caataagctg cttgcggacg
    10321 ccataatgca ttacaccttc gcgacccatc cgatacgtac ccgcgccttt ccgaacgacg
    10381 aactccgcga aattgccatc accgagcatt ttgccatgat tgatgccatc gcccgcgggg
    10441 acggaaaggg cttgggcgag atcatccggg cccacatcac gcagccgaag gaattctacc
    10501 tgcgcgccac atatatccag gacatcctgt gagtatcagc gtgcgccacc gatgcacgct
    10561 gaattgatcg gatgtcgcgt ggccgagctt ggctagcgcg gttcacgcca tggtggaatc
    10621 gggacggact ccgatcttgc tggatttgtg agtcgctatg cggctggagt ggcggccagc
    10681 atcgcatggc actcgtctcg acgcggttca cgagagggga tcgacccaac ggcgtcgggc
    10741 gcgaattctc atgcatgttc attcgggcag tttcggctcg gagtcggatc ggtcgctccc
    10801 agcttctcaa tgactcctcg aatgaacctt gttggcgtgt gaaaggcccg gtccaccggg
    10861 cgaggccgga gggctagcct atgcgggcta ggtttatgcc cgttaggcaa actttgcctg
    10921 cggacttggg cgcggcaatt atctgatggt caacggatag cctgccgctg gatggcgttc
    10981 gggaaggctt acgcgatcct agccttctgg cctgatgact gagatgctgc gtgaatcaga
    11041 caaagtggcg cttccgcgtg ttgtcggccc tggagacgct agagctttct agcgacgggt
    11101 attaagattt cactgccctg cccagctgtg aggtgtcatc aggttgtcca tccggcccgg
    11161 accgggaaag ctgaggttgt cgaagcttca gtgattccag ggggaccgga tgaacattca
    11221 taagaatgcc cgcctgacgc cgcacggtcg agagcgaatt gtgaggcagg tcggagagtg
    11281 ggcagacgcc gcaggccgtt gccgaagccg caggcgtctg cccgcggacg gtccgcaagt
    11341 gggttagcgg ctatcgccgc gaaggattgg ccggcttgca ggattgctct tcgtggcccc
    11401 accggttgcg ccggcccacg cctgagccag tgatcgcgga gatcgaacgg ctgcgccgcc
    11461 aacgttggac aggcaagcag atcgccgccg aggttggcgt ctcgcgactg tcagccgacg
    11521 gtgaagccga aggacatgcc gccgatgtcg ccccgctcgg ccaattcgag gacgtcacgg
    11581 ccagcggtcg tgttcggcac gtcgaggtga aagcgagacc cttgctgtct tcggagaggc
    11641 ggagcgtgcc gcttttcgtg cgccagaacc gctttcggat cgtgatcagc caaggccagg
    11701 atatcccggc cctggcgcaa ggtttccgtg aaggcgccgg gcagaatcac ttcggtgaag
    11761 tcggagatgc gggtatcgac gccatagagc gccgtatacc cctccaggcg gcggcctttc
    11821 gcccgcagct ctatcgaagc ccggcgctcc atcgaggatt actccccggc gctcacgtcg
    11881 aggtcgtcgg cgaaggcgaa ggcctcggcg tggcgcaccg gctgcgcagg cgcgccaatc
    11941 cggggcgtct aaccaggtct caagtttgct tgatcagcat cgacaaggtc aatgcgaggc
    12001 agatctggtg caagagctgg tcagcgccaa ggacaataaa aaatgcgcgc cattgtccgc
    12061 tgccaaaagt gggcagcgat cctcgaggtc gtgaaatcga cagccatgtg gagcagtgcg
    12121 ttggcgatga caaacgacac cagcgcgccc ggtttcgcta ctccaaagat cacgccggcg
    12181 cccacggcca atgtgatgct gtagatgatg acgtgagcaa tcagcgcgtc caggcggtcg
    12241 ctcttggctg tggactcaat ggcacagagc cagattctgg ttgaggcgag ggcgacggcg
    12301 gtgaggaaat tcctggcgag cttgtcgaag cgggttgcga tacgccggaa ctgcttgagc
    12361 ttgcagaaga agcactcgac gagattgcgc tggcggtaga ggtttgggga gacggagcgc
    12421 tggattttga cccgccgctg ggcgggaatg tgtgtgcttc cccccgcggg cggcgaccag
    12481 atcgatgatg gcgcggctgc cgtagtcacg gtccgcgacg accaccggcg atcaaacggc
    12541 tgtcatttgg ctgcggccaa tgtcgaagca tgaaatcgct ttcgcagaat cagcggggcc
    12601 gagccggcag tccgtagaca atgtggctca tcgctgggac cggactgcct aaattttatc
    12661 ccagcgctga ttccccccag gctgacctgc ctgttgtctc gacggccgtg cctgtaacgt
    12721 gcaggatatc cgtgcaagaa gcagcgtaga ggtcttgctg caacccaatt gttcgctgtc
    12781 tggacgccgt tcagtcatcg cgggttcaag gcaacattga cgctcgttgg gacggcggtc
    12841 caacgagcgc ctctccctgg agaactatgg ggtgggcctc ctccagggaa ttgagcgcag
    12901 gccgacccgc cttttcagtc gctgggctgc ttggccttgc gcagataagg caggacggcc
    12961 tcgaaggcgc caaagtactt caccgcgtcc tcgtccgata ccgagcccgc gatcacgata
    13021 tcctcgcccg gttgccagtc ggccggcgtc gagacgggaa acttcgcggt aagctgaagg
    13081 gaatcgaggg cgcgcaggat ctcctggaaa ttccggccgg tcgtcatcgg ataggtgagg
    13141 atcagcttga tcttcttgtc gggaccgatg acgaacacgg aacgcacggt ctggttatct
    13201 gccggcgtcc ggccttccga actgtcacct gcagaggccg ggagcatgtc gtagagcttg
    13261 gcgaccttaa gatccttgtc gccgatcatg ggatagttga ccgtgtggcc cgtggccttc
    13321 tcgatgtcac ccttccattt gtcgtggctt tccaccgggt cgaccgaaat gccgatgatc
    13381 ttggcattgc gcttatcgaa ctcgcctttc aagccggcca tggttccgag ttcggtggtg
    13441 cagatcgggg tgaagtcctt ggggtgcgag aacaggacgg cccagccgtc cccgatccaa
    13501 tcatgaaaac ggatcgtccc ttccgtggtt tcggctgtga aatcgggggc agtgtcgtta
    13561 atgcgaaggc tcattgagtt ctcctcggtt gcggcacgag gtaaacccgc gccattgaaa
    13621 caatcaaaat ttcgtccgcc gacaggtcga cgaacgatgc gcgaatgcct tagcagcttt
    13681 cgctggaggt cttttaccgt tgttgccctg gtgtgctgtg gagggtgtat gcaatctcac
    13741 tcgagcggca ccagcatggt gcaagccggc gcccaggtgc tgttcgcctc gactggagac
    13801 ttggtgaaga actccagcgc cgggacatcg gaatattggt agttcgtcct cggcatccat
    13861 ttgtcgagga tataggcata gacctccggg cgtggacgag gctctcggtt gttgtcgacg
    13921 acggcataaa gccccgcatc ggtcgtcacg aggcaatcct tcacctcgtc aagctgaagc
    13981 cgggcatcct cggcgtccgc gaaaacatag cagcagtcat agcggatttc gttcgggggt
    14041 gtcacacgcg gatcgtcata gacgcgcccg agaaaccggg ctctgcccgg ctcagtgcct
    14101 tgcaaggcat ccggcagccg ttcaataaaa tcccgccagc tatcctgaac cgtttcatac
    14161 ggtccgtaat agcgtctgaa caggcagcga taggagccga tgtcgcgaac ctggaccttg
    14221 gggggatcgt caaatgataa gccgtcgaac cggtctttat aaaagatttt cggatcccac
    14281 tcgtcgctcg accggtattc cgtaggagaa acgccaaaaa acgctcggaa cgccctggta
    14341 aaggctgcat tgctgctata cccataaccg atggcgattt cgtatattga ttgattcgtc
    14401 catttcaggc ggcatgcggc gttgtgcatt tttacgcgtt tgaaataggc atagaaggat
    14461 tcgcctacct cggtttggaa aaggcgctca aaatagaact tgcttagacc aacccttgca
    14521 gccatctctt caagttctat ttgttcgtta atattttgtt caacgtattt gagcgcagcg
    14581 agtatccgcg catccattga ttttggcccc ctatgataag atgcctcgca tcgtttcgga
    14641 ccgtctgcga tacgtctatt tggccgaagc agatcacaaa agcctgcggc gacaacaaaa
    14701 tcgcttgacc gactgatgaa gtcgttcagc ggtacacagc tttaagtctt cacattctgc
    14761 gtttgaaaat tctttctgcg acggatgtca agtatgttgt gacagcagct ttcaagatac
    14821 taactttatt agatttttct aaaaactatt cgggtaggtt ttggttctaa gttttccatt
    14881 cacttgctgg aaatcgccgc aactctagat atgggctgca atgcagctcg acctcacatc
    14941 gctattgctg gcttggcaag tcgtctcgat tgctcgtggc gtcatgattg tcgtggactc
    15001 ggcgtatggt cgggcttcgc tcggacttcg gttcgccggc gctttcctga gcactccggt
    15061 ggctaatgcg ccagcctcac cgaacctctc gctgtagcgg accgcatccg ccgcgacctc
    15121 tcagagtcga aacagaagta tttcgcgcgt acgcccaacg aggagtatcc gatcgcgacc
    15181 tgccgggggc gagtgtgccc ctgttcaaac ttttagaaca ggcagagcag gcgctctacc
    15241 gggcgaagaa ggagggcgcg acagagtcgc agtttatgcg aagcctcgct cgatcagcga
    15301 cgacaacaac aataacaacg ggcccgatcc aaccgcctgc gtatcgtcca aggcgagagc
    15361 cggcatgtgc aggccggctg gctctcgccg gctgtcacaa gctgtcaagc gggattttca
    15421 gatatcgtct tccgtttgcc tcgggcagcg gcagttgtcc gccattgatg ttgacttgca
    15481 gcgcatgaag gatcagccgg ggcatgggca aggtgcggtc gcgagcttct cgcatcgcca
    15541 caaactccgc cttcgtgcgg tagtgggaga tgtggatgtt ctctgccttt tgctcagcaa
    15601 ccgagctttc ccattttgga tcacgaccgc cggggcaata atcgtgaccg acaaagatgc
    15661 gcgtgtcatc cggaagggcg aggattttct ggatcgagtt ccacagcacg gccgcatcgc
    15721 cgcctggaaa gtcgcaacgt gccgtcccgc tgtcgggcat gaagatcgta tcatgaacaa
    15781 acgcacaatc tcccaccaca taggtgacgg aggcgagcgt atggcccggt gagaacagca
    15841 ccttcaccgg catcatgccg atccagaacg tgtcgccgtc gtcgaacaag acatcccatt
    15901 gcgaaccatc ggcccgaaag tccggccaat tgtaaatcgc cttccagagt tcctgcacat
    15961 cgaccacgtg ttttccgatc gccgtggcaa tcccggtctt cttcttcaga tatgcggcgg
    16021 ccgagaaatg atcagcatgc ggatgcgtgt cgagaatcca ttcgagcgtc agctgctgtc
    16081 gctcgatgaa atccagaagc aggtcggcat tggcggttga aacccgcccc gatctctcat
    16141 cgaaatcgag cacagggtcg ataatggcgc acctccttgt aatcgggtcg gcaacgacat
    16201 actggatgga acctgtcggc ctgtcgtaaa aggccgtcac ctctggcggc tcaatgctca
    16261 tgatctaact cccccggtaa ttgacaattg atgccccaac agaagatgta atttgtcgag
    16321 caaccatcgt cgatccgatt ggtctcatga ccgaatcgaa atccaacgag accgcggatc
    16381 gcacgaagta gctcctccca gccgactaca tggccgcagc tatactttcg agctgttagc
    16441 ttagcagctc tcgctaatgt ggtttgacca atcttgccgc atgacggcgg gctaccgtgc
    16501 gcctgatcgt tctaatgctc gatctcgtcg gcagtgcgcc gtcgcctaga gcagacgggc
    16561 tgttacctgc ctgaggccag tttgccgctg gtcatggctc agatctgttc acgctgccga
    16621 tcaggcggcc agcgccacaa ggcgctggta ggcggccttg aagcccttga gcgagatgtc
    16681 agccctgatc tgttggccgg tggtggccgt catcaggacg cttgccgtct cggcggcttc
    16741 cagcttctcg atcgtctctg caggaacctc cacttccgcg atgcagccgg tgggcaggca
    16801 ggtgcggtag gtgccgcgca gaacttcctc ctcggcaatc tcgacacgga acccttccga
    16861 aagcagaagg ccgaagggga agaccagggt cgcccttgcg gtatcatcgc gcctgcgcag
    16921 cgcaaaggtc aggacccgct gctgcgatcc ctgctggaga agggtctgcg ccatctggca
    16981 gacccgctgg gttttctcgc cctgctgttg gtttacgcac cgcaccgtcc acgcctcata
    17041 ggtttctgag agcgatgtag ggctttgcat ttcaactcct tcctgggcga gggctgcatc
    17101 gaggggcatc aggcagattg acgaaacgaa aaggcgaagg acaagtgcgg ctttcttcat
    17161 cggtcctctc gattgaagga ggcgggagcg ggggctgggc atggcaccag ccggtggacg
    17221 aggcgtcttc atcgaggctg cgaattccct agaacctgac attgagtgtt gccttgacgg
    17281 cgttctggga tgtgttcgag gcgaactggc cctggtagga gatgccgagt gtggcggtgg
    17341 gcgagaggtc gaagtcgatg ccggcctcga ggagggccgc atcctcgg
//
LOCUS       DASVDU010000216        35302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_297537, whole genome shotgun sequence.
ACCESSION   DASVDU010000216 DASVDU010000000
VERSION     DASVDU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 35302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35302
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297537"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VF226_13800"
     CDS             complement(<1..583)
                     /locus_tag="VF226_13800"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7075109.1"
                     /translation="MKDAECIAFLQWALPQINLRWAGFRKVRRQVCKRIARRLDQLGL
                     ADLDAYRARLAIDPAEWSALDAMCRVTISRFYRDRGVFQTLAHAVLPNLADEARAQGR
                     DVACWSAGCASGEEVYTLKIIWDAQVSPHHEGAGLTVIGTDVDDAVLERARVGCYQRG
                     TLREMPSELIARCFDVRGDRLCVKERHRRGVTFL"
     gene            complement(616..1389)
                     /locus_tag="VF226_13805"
     CDS             complement(616..1389)
                     /locus_tag="VF226_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX7075110.1"
                     /translation="MLIFQSACQASQGARRYQEDTALSWPEEASAGEGAEQLVAVLAD
                     GMGGHVGGALASRLVCETFIATVTTAGGASRERLAAGLHAANAAIADQIAVNPVLRGM
                     GSTLVGALFGPDGLEWVSVGDSPLYLYRDGEIALLNEDHSLAPVLDQLAAEGRLSLTE
                     ARNDPRRHLLRSAVTGEDLDLVDLSRQPLPLQAGDCVLLASDGVHTLEADEIARVVRA
                     YLDEGPRAVAEALVRTVEEVRDPHQDNITVLVVKPIERA"
     gene            complement(1389..2177)
                     /locus_tag="VF226_13810"
     CDS             complement(1389..2177)
                     /locus_tag="VF226_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7075111.1"
                     /translation="MADEYDGQGPRAPGKFFGSLREALSAAAKDDTGRQAPIALGQST
                     PADPEPPTQEPAATLSAVEPPEPTQIMRTARPLPSAAEAARAARGGTPNIPPVDRHVE
                     FDVPPTTRVVKPEPMAHPEPPARTQLVRGRQPVKRGRFYQDPVVGWLVVVGGPGLGAY
                     RPIYEGNNTVGRSPSQRIPIDFGDEAISSEEQAYIRYDSADRSFLFVPNLAKTNVVSV
                     NEKRPTSAVPLVAMDVITMGRTQLVFVPFCGPEFDWSELPDIKD"
     gene            2462..2905
                     /locus_tag="VF226_13815"
     CDS             2462..2905
                     /locus_tag="VF226_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7075112.1"
                     /translation="MGVKVRRVETRDKPFWLELFKGYLAFYKATIPEEVIELTWQRLI
                     AGEPGFHIGLVATKEDDVPVGLAHVLFHRSTWSPTWYCYLEDLFVDPSERTKGIGRAL
                     IEAVYKEADARQCTRTYWATQEFNYRARALYDQLATKSPFIQYRR"
     gene            2929..3390
                     /locus_tag="VF226_13820"
     CDS             2929..3390
                     /locus_tag="VF226_13820"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7075113.1"
                     /translation="MQQTDRLWLLDTQVRILVSCDDESHGITIIEHEAPFGDSPPLHR
                     HLREDESFHVISGTLDIVTDGEMKRLGPGQTLRAPKGGSHTYRVVSSDGARFLTISGG
                     RDFEQFVRRMGRVPTGPGLPERSGPPSEEAVAELVRICAEHGIEVVGPPLT"
     gene            complement(3420..4718)
                     /locus_tag="VF226_13825"
     CDS             complement(3420..4718)
                     /locus_tag="VF226_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075114.1"
                     /translation="MVTTSQAAVMALALTAGAVVLGTLDASAQTSRRDGISELLAREG
                     QPDGQPNAAQTQAAERDAREGEATYEQARKLMRALDAVLEDAARNRTEARKLPSRSEF
                     VVTPLWTETREDRERKIRDLLDAALGIVTDVPVVDIQRRVETLRKNIRDLEDRIVKLK
                     EKQLVAPEDSLLPGVLTDTVDSLGREIEEAKRRIEENRKAIEAAKTEIHQALAASGVE
                     LSADQVDLLLDSVLAGDLVRLTAVFNSAKLIDRQLAKLLSASGDNMNAARKYFAMHAA
                     LFAMLVHAQDTLIAKIDDNYLPKLDEIAKDIEVARRKTMELLKAENRPDQRRALEANR
                     ESQKLAAEAAKGYRRYLLQQRQQISEARRRAAHDLRIADNTYETVEASFQLRNLMRDS
                     SASFEAIQKLEAPTFEQIFRNEELRKEFENLTRRLDAPSS"
     gene            4964..5410
                     /locus_tag="VF226_13830"
     CDS             4964..5410
                     /locus_tag="VF226_13830"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7075115.1"
                     /translation="MSTFEPLDPDYEARVRSSFARQRFMRFLGAEILRIAPGEVDLAL
                     PSRENLTQQHGYFHAGATAAIADTAAGYAALSLYPKGTGVLTTEFKINLLRPAQGRRL
                     IARGRVLKPGRTLSICQSDVYGERDGRETHVATLLLTMMCLEGLQD"
     gene            complement(5414..5902)
                     /locus_tag="VF226_13835"
     CDS             complement(5414..5902)
                     /locus_tag="VF226_13835"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7075116.1"
                     /translation="MSQTEHHSSADRPSPFPWPPVLLVLTVVAAWVLQLLVPLSWPGM
                     DDTASHIAGLAIGVAGIALMAWSIITLRRARTTVMPTEAASTLVTDGPFRFRRNPIYL
                     ADMLILLGIAELMRNIWLVLLTPVFGILVTWLAIIPEERHLEARFGDAYRAYKARTRR
                     LI"
     gene            6136..7308
                     /locus_tag="VF226_13840"
     CDS             6136..7308
                     /locus_tag="VF226_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX7075117.1"
                     /translation="MIPNALPSFNFDLGDTADQLRETVRTFTSDEIAPIAAEIDRTDE
                     FPRHLWPKMGALGLHGITVEEEFGGVGLGYLEHVVAMEEVSRGSAAVGLSYGAHSNLC
                     VNQIRRNGTPEQKRRYLPKLISGEHVGGLAMSEPGAGSDVVSMKLRAEKKGGRYILNG
                     TKMWITNGPGGDVIIVYAKTDPDAGPRGITAFIVEKNFKGFRVAQKLDKLGMRGSNTG
                     ELVFEDCEVPEENVLGTVNKGVNVLMSGLDYERAVLAAGPLGIMQACMDVVIPYVHER
                     KQFGAPIGTFQLVQGKIADMYVTLNSCRAYVYAVARACDRGETTREDAAGAILLAAEK
                     ATQVALDAIQLLGGNGYINDYPTGRLLRDAKLYEIGAGTSEIRRMLIGRELFEKTA"
     gene            7462..7932
                     /locus_tag="VF226_13845"
     CDS             7462..7932
                     /locus_tag="VF226_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEX7075118.1"
                     /translation="MRFKAAAAALLAPALLMGCGPDGPTKADTGLAVGAIAGGILGNQ
                     VGKGSGRVLATVAGAVVGGIVGSEIGRALDRQDQMLAQEAEFEALERGRSGERRRWRN
                     PESGRYGEVIPGERYRRGTADCRDFTHTIYINGRPETMRGTACRNPDGTWQNVG"
     gene            8071..9222
                     /gene="ccmI"
                     /locus_tag="VF226_13850"
     CDS             8071..9222
                     /gene="ccmI"
                     /locus_tag="VF226_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HEX7075119.1"
                     /translation="MLFWVLCAVLTAAVLYFVTRPLTVPVAGPSALERDRASDLAVYR
                     DQLSEVEADRARGLLSDAEAEAARIEISRRLLARAESGEPAQGGSGERGLTGARMAFI
                     TAVILVPAVSLALYLAYGSPGLPGRPHAERVAASADKATIETLVAKVEERLRAHPEDG
                     QGWDVIAPVYFRQGRFDEAVQAYRNAIRLLGESPKRLAGLGEAQVFAKNGIVTEDARR
                     AFQRLAELEPSRPEPRFWLALAKEQDGDIGGALADYRKLVADAPADADWRAPVEQRIA
                     MLTQRRAGDPRDEAERGPTEQDIAAAGKLAPGERAAMIEQMVEGLAQRLETESQDLAG
                     WQRLMRAYAVLGQKDKANDALAKARKAFAGDETSLAALNDLARKLGLDS"
     gene            9241..9699
                     /gene="ccmE"
                     /locus_tag="VF226_13855"
     CDS             9241..9699
                     /gene="ccmE"
                     /locus_tag="VF226_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX7075120.1"
                     /translation="MTRKQRRAILILACVGLLGLASILVLFALRDSIVFFHTPSEVAE
                     KGIEAGQRFRLGGLVAEGSVRRGEGAAVEFAVTDTVKTISVVYRGVLPDLFREGQGVV
                     TEGRLDPSGKFHADTVLAKHDENYMPPEVAGALKDKGVWQHMGKQQPATN"
     gene            9715..11706
                     /locus_tag="VF226_13860"
     CDS             9715..11706
                     /locus_tag="VF226_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEX7075121.1"
                     /translation="MIVEIGHFALILALAVALIQATMPLWGAHIRDERLMAVGPPAAL
                     AQLLLVAIAFLALMYAYVWSDFSVENVYLNSHSAKPLIYRISGLWGNHEGSMLLWILT
                     LSLFGAAVAVFGGKLPETLRANVLGVHGAIAVTFILFIVLTSNPFKRLVPAPVEGLGL
                     NPILQDPALAFHPPFLYAGYVGFSIAFAFAIAALIEGRIDAAWARWVRPWTLAAWMML
                     TLGISMGSWWAYYELGWGGWWFWDPVENASFMPWLAGTALLHSAVVMEKREALKVWTI
                     LLAILTFSLSLMGTFLVRSGVLTSVHAFAVDPARGVFILGILVIFIGGALALFALRVG
                     ELRQGGLFAPISREGSLVLNNLLLTTACATVFIGTLYPLALEAFTGDKISVGAPFFDA
                     TFGPLMLPLLVALPFGPYLAWKRGDLTAATQRLWAAAAVTLLAIIVAYAVAYPGPWLA
                     PLGIALGVWVMAGALSECASRIKLGQVPLAESWRRLTGLPRAGFGMSVAHFGVGMMVV
                     GIIATSAYQSERILVMKPGDQVEIAGYTLTFKGAAPQRGPNYREEVGLFEVERGGRVV
                     TNLTPSKRMYDAPPQPTSEAGIHVSWRGDLYVVLGDQQTDSSGGYAVRLYFNPLVRFI
                     WTGAVVMFLGGLLSLSDRRLRVGAPRRARRVRAAAVPAE"
     gene            11711..12235
                     /locus_tag="VF226_13865"
     CDS             11711..12235
                     /locus_tag="VF226_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEX7075122.1"
                     /translation="MMARPCRAVLWLLVFPFLFLGAAASLAVEPDEMLADPALEARAR
                     ALSAELRCVVCQNQSIDDSNAPLARDLRILLRERLAAGDSDEQVKQFLVDRYGAFVLL
                     RPPFNAHTLLLWLAPPIILVGAGVLLFRYMSGRGRTAPAEVSQSLTAEEEEKLRSLLA
                     AESPDAGSAGRENR"
     gene            12367..13914
                     /locus_tag="VF226_13870"
     CDS             12367..13914
                     /locus_tag="VF226_13870"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7075123.1"
                     /translation="MSTENDKVRATRIRMPRRTKGPLVAALAGVSALALVTVSPSIPV
                     RAQFNPQGSGVETLGRAPLSFADIVERVKPAVVSINVIEGEARVARNDDDKNLPDGFP
                     NLPEDHPLHDFFKNLPREFRMPMPRPMPRQRQATGSGFLISPDGYVVTNNHVVDGATK
                     VTVSFEEQEKYEADIVGTDPRTDLALLKIKVGNGKQFPYVKFASKPPRVGDWVLAVGN
                     PFGLGGTVTAGIVSALARDIGSGPYDFMQIDAAVNRGNSGGPTFNLNGEVVGVNTAIY
                     SPSGGNVGIAFAVPAETASEVVTQLKERGTVRRGWLGVRIQSINEDIAAGLGLKEPKG
                     ALVNDVTPNGPADQAGLKSQDAILAVNGDPISDSRELARKIAEFAPNTPVDIKILRNN
                     KEMNVKVTLGTFPDSKEEIARLEQGGPAGSEMNALGLTLGPAPARKGGKIEGVQITDV
                     DSDSDAAAKGLKSGDVILEVNSREVKSPADVEEAVKEAKELNRKAVLLTVRSGDQKRV
                     VAVQMKG"
     gene            14021..14701
                     /locus_tag="VF226_13875"
     CDS             14021..14701
                     /locus_tag="VF226_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7075124.1"
                     /translation="MRVLVIEDDREAAHFLQKALKESGHAAVVTHDGEEGLEAAREGK
                     FDVLIVDRMLPKLDGLSVVRLLRDEGDRTPVLILSALGEVDDKVKGLRAGGDDYLTKP
                     YAFSELLARIEALSRRSDPQQEQTRYVVGDLVLDRLSHRVTRAGEPITLQPREFRLLE
                     YLMRHAGQVVTRTMLLEHVWDYHFDPQTNVIDVHISRLRAKIDKNFEESLLHTIRGAG
                     YMIRAGKS"
     gene            14685..16154
                     /locus_tag="VF226_13880"
     CDS             14685..16154
                     /locus_tag="VF226_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7075125.1"
                     /translation="MLARANPLLPHFTRTTAFRLMAAYVAVFVTVAAVIAGVILWQTN
                     QLLIRQIFATLRTEAESVQDAVRQRGPAAAPDIIAERSRLSEGGLYFLADPQGHKLAG
                     NLNRWPPELRDQSGGLFRYAATTGEQSRERVAIGIPVALERGQRLLVARDVEEQRQFI
                     GRVRSLFLAGFGLLVLLGLGGGLAASRLILRRIAKITTTARSIMRGDLSRRIATSGTG
                     DELDELSRNLNAMLDRIEQLMAGLKEVSDNIAHDLKTPLNRLRNRAEAALREARSAED
                     YQEGLERTIDEADEIIKTFNALLLIARLEAGAVDESKEIFDLTALVRDLVELYEPHAE
                     ENRRKLAFEAEQGEILVRANRQLIGQAVANLLDNAIKYGGPAPDSPNGGSASDIEVRL
                     SRTEGGVEIVVADHGPGIPALDRERALKRFVRLEASRTRPGTGLGLSLVAAVARLHEG
                     QVKLEDNKPGLRVVLSWPIPAMLERPETKVAVSQTRIAQ"
     gene            16151..19090
                     /locus_tag="VF226_13885"
     CDS             16151..19090
                     /locus_tag="VF226_13885"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEX7075126.1"
                     /translation="MSTLASAPASLQDRLRQAPFGAEDPHIAEAVRELEAEMADASRA
                     AGSSMTEQARQLLGGVLAGSPYLSGLMRRDPARLVRVLSRAPEESRADLLESVARQAT
                     AAQSVTELMAALRIFKNEVALLTALADLGGVWDLADVTRTLTLAADAAVEHAVRFLFR
                     RAVANGTWAAPDHAAPDRDSGYIVLGMGKYGAQELNYSSDIDLIVFFDKQRLKLRADL
                     EPQTFFVRMTRELVRILQERTGDGYVFRTDLRLRPDPGATQVALSTDAALTYYESFGQ
                     NWERAAMIKARPVAGDIAAGRALLSELSPFIWRKYLDFAAIADIHAMKRQIHAHRGFG
                     KIGVAGHNIKLGRGGIREIEFFVQTQQLIAGGRQPDLRTPQTLVTLDRLAERGWIKPG
                     VRDDLARSYTYLRGIEHRLQMVADEQTHSLPNAPEALASFARFAGYPSTDAFSKELVA
                     HLEAVQRHYAALFEDMPELTRGGANMVFAGSADDPQTVEALARMGYSQPHQVIDTVRG
                     WHHGRYAAVRTAQARERLTEVQAVLIEALAQTANPDSALASFDRFLAQLPAGVQLFSL
                     LRANPNLLRLVADIMGSAPRLARILSRRRRVLDAVLDPRIIGNLPTPEELDALIRDEI
                     ESTDDYQEILDRARIIGNEQMFLIGVRVLSGIIVPSQAGGAYALLADHLIRALLKAVE
                     RDFARLHGRVSGGQAAVVAMGKLGGREMTAASDLDLILVYDFAPDAVQSDGSRPLAPS
                     QYYARLTQRLISALSSPTAEGQLYQVDMRLRPSGQQGPVATQLSSFIEYQAHQAWTWE
                     HMALTRARVVAGAPELRAKVEQAIRDALVQPRDRAKIAADVHDMRQRIAAEKGTDEIW
                     DLKQVRGGLVDLEFMAQYLQLIHAAEHPDVLDQNTVRALQKLDGKGLIPGGAGTILIP
                     AAQLINDLTQIVRLCVDGPFRANEASQGLKDLLARAAHAPSFERLEADLRLMLAETAS
                     LFDQIVS"
     gene            19171..19986
                     /locus_tag="VF226_13890"
     CDS             19171..19986
                     /locus_tag="VF226_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX7075127.1"
                     /translation="MKKKTMILIAGVLIAAGGAAAVAGVGEKRARFGHGPGTGAMFAH
                     MGGHGFGFGRGGGLKALDADGDGVITIEEALAARSPVFARIDTNGDGVIDAQEIEAEV
                     NLNTQYWTQAMFHRRDRDGDGLISKEEFSSKKYRSERRAERAEDGKDDDRSDRARRGH
                     RYGWHGHGHHHHRWHHSRWRGDRMASRFEKMDLNANGTLEASEVETAIKPRVTRRVNR
                     MISRFDQDNDGRVTREEFEKPARDRFAMRDINKDGRISEEDLPPMMRGRGILR"
     gene            20185..20808
                     /locus_tag="VF226_13895"
     CDS             20185..20808
                     /locus_tag="VF226_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7075128.1"
                     /translation="MRGKWGEQGGSSAGSVGGPAGDSTTTDASLLARAGRGDAAAFRE
                     LVDRHLPAALGIARRMLQDDAEAEDVAQEALLRLWRTASDLEVGPAGVRPWLRRVVSN
                     LCIDRIRSSQRTDVTDEVPDMPAETNQFRELTQQDLSARVDEALKNLPERQRLALTLF
                     HYEGMSHLEIGEVMGVSVEAVESLLGRARRTLKATLKNDWRQLLSEA"
     gene            20829..21344
                     /locus_tag="VF226_13900"
     CDS             20829..21344
                     /locus_tag="VF226_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075129.1"
                     /translation="MAGDKNSADLTALEEHLEIHGSRIERWPDAARRRFDLLLKENAR
                     ARELLAEARALDDLLDRAPLPDARRMADLTERIAALAKAEGREQRTAAPVIDLTARRQ
                     ARRPPVTPMRWKVASALAASLLAGIYLGSSPPVSTAVESVASLVGVAPDNESSDLVLI
                     YDPAADEEEFL"
     gene            21341..21841
                     /locus_tag="VF226_13905"
     CDS             21341..21841
                     /locus_tag="VF226_13905"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX7075130.1"
                     /translation="MSEPEGSGAASARRVPRWLWAVLIVSLGLNLLVAGVVASAAWHF
                     HSLNGSRGRLSAYLETLPPQRSEFLQGIRERFQPVLRPLRQEIRETRREAARLFAADP
                     LDKQALVATHQRLMDAEVRIRQSYTQLMTELAENMTPQERRDFIEWREQRRRGSHRSA
                     NLSSQQ"
     gene            complement(21898..22836)
                     /locus_tag="VF226_13910"
     CDS             complement(21898..22836)
                     /locus_tag="VF226_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEX7075131.1"
                     /translation="MHVVCLGAGGIGGYFGGRLVEASAANVTFLVRPGRKAQLQKDGL
                     RVESVHGDFTVPVDARLAEEIRSPADYVLLTCKAYDLDSAIEAIRPAVGPNTAILPLL
                     NGMSHMDRLNEVFGRERVLGGMAQIAITLQPDGLVKHLNDWNTITFGEQNAASSPRVE
                     ALKAAFDKTSGKANAVAVPDIIQKMWEKLVMLSTLASMTTLMRANVGEIARTPDGSGL
                     TLAFLDRNAQIAAAEGHPMPEEFIERFGKVFTDTGSQMAASMLRDIERKGPVESDHIV
                     GFMLEKARAHGIDDTLHRISFTHLKAYEQRRAADRL"
     gene            complement(22934..23251)
                     /gene="trxA"
                     /locus_tag="VF226_13915"
     CDS             complement(22934..23251)
                     /gene="trxA"
                     /locus_tag="VF226_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7075132.1"
                     /translation="MVSDSDFATEVLKSPEPVVVDFFAEWCGPCKAMAPALEQVAQEM
                     KGKVKVVKVDVDRSPEVTQQFRIQAMPTLMLFKDGKVANQHVGALVQKKRLEDWINQS
                     LEG"
     gene            complement(23336..26884)
                     /gene="addA"
                     /locus_tag="VF226_13920"
     CDS             complement(23336..26884)
                     /gene="addA"
                     /locus_tag="VF226_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HEX7075133.1"
                     /translation="MTDAAMKALEETARAQRSASEPDTSAWVSANAGAGKTHVLKMRV
                     LKLLLSGTAPDKILCLTFTKTAAAEMAQRVFDELSHWATCPEPELRKTLSGLLGRSPS
                     AEEVRGARQLFAEAVDTPGGLKVQTIHAFCERLLQRFPLEAGVPPGFTILDDEMAGTL
                     LRQSIDAVLRTATSPGNEQLGKALETVVAYAAEDSFEKVLQDALGRRDWLEEMSRLGF
                     RDGDPLQEARQIYSAALGITTPSLSAVEQQLATVLPEPDLRHAAAVLATGSKTDARHA
                     GILEAALARTEAEQRAAELRRYFLTGGGEMRASLMTKGLQSSHPDLHATLIAAQQCFA
                     ALCGQRIAHKLADATLALWQLADAVRQHYAAAKARRAALDFEDLIRHTASLLKSSDSA
                     QWVLYKLDNGLDHILVDEAQDTSPSQWSIITGLAREFFSGLGAHEKTRTLFAVGDEKQ
                     SIYSFQGAEPKLFAVTGKVFEDTAAQAGLNWRTVPLTLSFRSTEPILASVDRVFSDHT
                     RTPGLTAAAEPVRHQARRIGQAGFVEIWPTEAFETIEPAEPWSPLADKPAQSPVIKVA
                     DRIANTIRDWLARGERLQSEDRPIRPGDILILVRKRQPFAAPMVAALKARGIPVAGSD
                     RMQLTEQIAVEDMIALGEFLILPEDDLALAAILKSPMFGLDDDDLLTFAPGRSGSLWN
                     ALLAAAKTNPRLEFAADTLRRWRAEADYRPPYEFFAGILDNEGMRQRMLARLGAEAAE
                     PIDEFLNLALQYDDRNPPSLQGFVTWLRRLHVEIKRDQDHGREEVRVMTVHGAKGLEA
                     PIVFLPDTCSSRSGRPHSALIPLPGVTKPMGFPDPYVWPVKETRDHPAVEAAKAAIEA
                     AETEERNRLLYVAMTRARDRLYVAGFETKSGRGKGCWYDLIEGALRDVCETVETEDGR
                     TVLRYACPQTAPPEEPRNEATRLASPEPLPDWAKRKAPREPATTFPLSPSRLARLETD
                     EIGDPLVARTSAPSMVSPSPLALSSEYRFLRGTLTHALLEHLPSFPVDRWADAAHSFI
                     AVRGESLPENVRSDIVEEVLSILRDPQFGSLFGPESRAEVPIAAAIPDPAGRRPAVRI
                     TGQIDRLVRLPGEVLLLDYKTNRPPPQQPEEVPKAYLLQLAAYRLVVGQVFPELPVSA
                     AILWTDGARLMPIPQELLDLHEALLWEHGSPQLDAR"
     gene            complement(26877..30050)
                     /gene="addB"
                     /locus_tag="VF226_13925"
     CDS             complement(26877..30050)
                     /gene="addB"
                     /locus_tag="VF226_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEX7075134.1"
                     /translation="MPPEGGARPAAPARKAEAAAGLFTVPPGQPFLPALARAILSGDL
                     PRQGCNPPDPLDLPKTTILLPTRRAARALQEAFRVASGKRALLLPKIRPIATVEEDLT
                     LLSELAAPPASGTASTDIPPAISELERRLVLTELVLKWSETMRSAHPTEHGDILPYTG
                     AGARTPAQAASLARELGRLIDMVETEGASLDRLPDLVPEPFSEHWQQTLEFLKIVLDW
                     WPKHLAERGLTSPMDRRNRLILAEAERLAANPPTAPVIVAGVTGSIPATAELMQVVLG
                     LQNGAIVLPGLDQTLDEAGWGRLREHPEHPQHGLSKLLSRLGVSRSAVKCLPGSEADG
                     PSMLRAAFVSEALRPASTTHLWSRFVETTDPGAVRDALRGVSLIEAPTAQDEAEVIAL
                     ILREALETPERTAALVSPDRILARRVVNRLESWGVLVDDSAGRPFRKTVPGTFLDLVI
                     DAFDRDFEPAAVMALLKHPLTLLGLSARDARRVARALELAAFRTDYFGRGLDGIEAAI
                     ERAAQQPRTKAQRQLGTSDWRAARDLLARLRTAFQPLTELSGRPGKQALAEFVRAHVA
                     VAEALAAPADAVGTVASPLWSEEAGEAAAVLLAGLIDPALVAPRLAAADYPDFYRNLV
                     ADENVRSALPAHPRLSIWGLLEARLQHPDVVVIGGLNEGTWPRISDTGPWLNRPMRDE
                     LRLPQPEARIGDQAHDFAQLLGARTVYLTRAEKVDGAPTVPSRWLLRIKALLEGMGLS
                     DALTPDRPWLGWARARDLVEPIPRLRPPCPCPQLALRPRRLSVSDVERWITNPYALFA
                     KRVLGLEPLPLLGAEPSAALRGGIVHEALGRFAACYPADLPDEPARELMAIAGKILTE
                     YTGNPRVAAFWVPRLERFARWFAATEPNRRAGVIRVLAEVSGSLVLSGPAGPFTLTAR
                     ADRIDVTENGLVITDYKTAGESQVKTLAKRAADGRSPQLPLEAVIALAGGFEGLGPSA
                     IAMLRYISASGAEPAGVEQSINAEIASLIDETRAGLERLIAVYDDPVTPYRAVRRPAY
                     DYRYDDYKHLARVAEWLGAEAEGGGDD"
     gene            complement(30040..30765)
                     /locus_tag="VF226_13930"
     CDS             complement(30040..30765)
                     /locus_tag="VF226_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7075135.1"
                     /translation="MTWIPKSAMVLAAGLGSRMRPLTDLVPKPLVRLRQKPLIDYALD
                     RIAEAGISQAVVNVHHFADQVISHLKTRQRPRILISDEREQLLDTGGGVKKALPQLGQ
                     EPFLIHNSDSVWIDGVQSDLVRLAQSFDAERMDCLLLLALSSASIGYDGRGDFLMSPD
                     GLLQRRPERELAPFVFTGVSIAHPRLFDGAPEGAFSLNQLWDRAIQQRRLYGIRLDGT
                     WMHVGTPAALAEAEQALGGSYAA"
     gene            complement(30795..32306)
                     /gene="tsaE"
                     /locus_tag="VF226_13935"
     CDS             complement(30795..32306)
                     /gene="tsaE"
                     /locus_tag="VF226_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7075136.1"
                     /translation="MRETDIAPLAELLAYALRPGDVVALRGDLGAGKSTLARAIIRVL
                     LQDEAAEVPSPTFSLVQTYATPRFPVSHFDLYRLGSADEARELGLEECIATGAALIEW
                     PERAEELLPADRIEVHLEEDPSLDPSERRITIEGLGTLQPRIERLRIIDEFLQQSGWG
                     DARLRYLQGDASARRFARLSRNGDSAILMDAPRQPDGPPIRDGKPYSRIAHLAEDLRP
                     WVAVTSTLRSAGLSAPRVIAQDLDAGLLLVEDFGDNVFSQIAGQEGVDQAELWRSAVD
                     VLVHLRSVPVPRHIRLSDGSSHQLPRYDRAAMQIEVELLLDWYWPALYGQAAPDALRS
                     EYLAAWNGIFDDLLPLPTGLVLRDYHSPNLVWLPDRSGLERVGILDCQDAVEGHPAYD
                     LASLLQDARVDVPQALEHELFDHYCAAMASQDPAFDRVTFAFAYAALGAQRNTKVLGI
                     FTRLSMRDGKNGYLRHIPRIWGYLERDLAHARLRPLKAWYDANFPVAFRIPPD"
     gene            complement(32368..34857)
                     /locus_tag="VF226_13940"
     CDS             complement(32368..34857)
                     /locus_tag="VF226_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7075137.1"
                     /translation="MADPQSRTDATRREILALLLASLACIFILTLGLVETRASGVAGF
                     SIRQLLFAALATGAAGLGITILWYMLRRARLAIRRAETEAASLRRHLTTAEAIVKAEP
                     QVLVFWEQGQPLKVVSHTLTSVAGLPENQKEFLRFGQWLDTDSAEALKQGLDALFADG
                     RPFNLLLKTVAGGHLEADGRAAGARAVLRMRDVAGYKRDLIKIIDQHRQLARDIRSSR
                     ALLNALPMPVWLKSPSGKIVWVNKAYVAAVDAADEAEVREQQIELLESRQRAALEPHI
                     AKRETVIKRMPLIVGGVVKPHDVITVPLEDTIASAAIDVAALETAQGELDRQIEAYDR
                     TLDRVSSAVAIFGPDQRLSFFNVAYQRLWDLDREWLESSPSDSEILDRLRETSRLPAI
                     VNYRDWKAKLLACYQTAAMLEDWWHLPDGRMLRVVVEPRPDGGVTHLFDDVTERYALE
                     SRYNALIGVQRETLDNLKEGVAVFATNGRLKLSNSAFASIWRLPRQVLEGEPHIDEII
                     RHACERFENRRTWMRISRAVTAISDHRDSVEGQMVRPDGSVIDFSAVPLPDGATLVTF
                     ADVTASKRIERALAERNEALVEADRLKSQFISHVSYELRTPLTNIIGFSELLSSPRAG
                     ELNQKQREYVSDISSSSKTLLSIIDDILDFATLEAGAVELKLARVDVRHVLDSAILGV
                     RERASRARLTLDIAVADDAVDFVADEARVRQIIYNLLSNAVGFSKPGDTVKVTCWREN
                     GMVVFSVEDQGIGIPKDLQHRIFERFESRSFGSKHRGVGLGLSIAKKLVELHGGTMQL
                     ESEPGRGTRVTVRFPEVTAKLTVAEAMAAQA"
     gene            complement(35087..>35302)
                     /locus_tag="VF226_13945"
     CDS             complement(35087..>35302)
                     /locus_tag="VF226_13945"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7075138.1"
                     /translation="MLAAALAMPWLGFLVAAIISFAALLLIAMHDPWTPSRIVVYPLV
                     GLAIVIGFYVLFAKLLMVPLPSGSLFV"
BASE COUNT         6064 a        11307 c        11890 g         6041 t
ORIGIN      
        1 tgaggaatgt aacgccgcgc cggtgccgct ccttcacaca gagccggtcg cctcgaacat
       61 cgaagcagcg cgcgatgagc tcgctcggca tttccctcag cgtgcctctc tgataacagc
      121 cgacacgggc gcgctcgaga acggcatcgt caacgtcggt tccaatcacg gtcaggccgg
      181 cgccttcgtg gtgcggcgaa acctgtgcat cccagatgat cttgagcgtg tagacctcct
      241 cgcccgacgc gcacccggcc gaccagcagg cgacatcccg gccctgcgcg cgcgcttcat
      301 cggcaagatt gggcaaaacg gcatgggcca aggtctgaaa gacgcccctg tcgcggtaga
      361 agcgcgaaat ggtgacccgg cacatggcgt cgagcgcgga ccactccgcc ggatcaatgg
      421 cgagacgagc acgatacgca tcgagatcag cgagccccag ttggtccagg cgccgcgcga
      481 tgcgcttgca gacctgacgg cggaccttgc gaaatcctgc ccagcgcagg ttgatctgcg
      541 gcagagccca ttgaaggaac gcaatgcact cggcgtcttt caagcgacca tcctgccgta
      601 cggccgggcg gcgtgtcagg ccctctcgat cggtttcacc acgagcacgg tgatgttgtc
      661 ctggtggggg tcgcggacct cttcgacggt gcgcacgagc gcttcggcca cggctcgcgg
      721 gccttcgtcg agataggccc tgaccacccg ggcaatctca tccgcctcga gcgtgtggac
      781 gccatcgctg gcgagcagca cgcagtcgcc ggcctggagc ggtagcggct ggcgcgaaag
      841 gtccacgaga tccaggtcct caccggtcac cgccgagcgc agcagatggc ggcgcgggtc
      901 gttgcgcgct tccgtgaggc tcagccggcc ttcggcggcc aactgatcga gcacgggcgc
      961 gagcgagtgg tcctcgttga gaagcgcgat ctctccgtct cggtaaaggt aaagcgggct
     1021 gtcgccgacg ctcacccatt cgaggccatc tgggccgaac agcgccccga ccagcgtgga
     1081 gcccatgccg cgcaggacgg ggttcacggc gatctggtcg gcaatggcgg cattggcggc
     1141 gtggagcccc gctgccagtc gctcacggct ggcgccgccg gctgtcgtga cggtcgcaat
     1201 gaatgtttcg cagaccagac ggctcgcgag cgcgccaccc acgtggccac ccatgccatc
     1261 ggccaggacg gccacaagct gttccgcacc ctcgccagcc gatgcctcct ccggccagct
     1321 cagcgcagta tcctcctgat accggcgtgc accctggctg gcttggcagg ctgactggaa
     1381 gatgagcatc aatccttgat gtccgggagc tccgaccagt cgaactcggg tccgcagaaa
     1441 ggaacgaaga cgagctgcgt ccggcccatg gtgataacgt ccatcgcgac aagcgggacg
     1501 gctgacgtcg gccgcttctc gttcaccgaa accacgttcg tcttagcaag attgggcacg
     1561 aacaggaagg agcggtccgc cgaatcgtag cgaatgtagg cctgctcctc ggaggatatc
     1621 gcttcgtcgc cgaaatcgat cgggatgcgc tggctgggcg agcggccgac ggtgttgttg
     1681 ccttcgtaga tcggccgata ggcgccgaga cctgggccac cgacgacgac cagccaaccg
     1741 accacggggt cctgatagaa tcggccgcgc ttcacgggct gacggccacg cacgagctga
     1801 gtgcgcgcgg gcggctcagg atgggccatc ggctccggct tcacgacccg ggtcgtgggt
     1861 ggaacgtcga actccacatg cctgtcgacg ggcgggatgt tgggtgtgcc gccgcgcgct
     1921 gctcgcgccg cctcggccgc gctcggtagc ggcctggcgg tccgcatgat ctgtgtcggc
     1981 tccggcggct cgaccgcgga cagtgttgcg gcaggctcct gcgtgggtgg ctcgggatcg
     2041 gccggcgtgg attgacccaa tgctatgggc gcctgccgcc cggtgtcatc tttggctgcg
     2101 gcgctcagag cctcccgcag cgagccaaag aacttgccgg gcgcacgagg cccctgaccg
     2161 tcatactcgt cagccatgct cgattccctt tccccttctg cccgggccgg cgcagcgggc
     2221 cggcccacct ctgctcgtca cttccctgga gcgtgagccc cggaaggcta ttttcggttg
     2281 aggcttgcgg ccatcggagc ctcacgcccg aatcgcgcga ctataatgct ccgtgttcag
     2341 gtaaaggtgt tcgcgcaaga gggcagaacc atggccagcg cgctggcacc tgactttccg
     2401 atatggcact ttgggaatgt ttctcgggat cgtgggtcgc gacagtcggg agtctggaag
     2461 agtgggagtc aaggtcaggc gggtcgagac ccgcgacaag ccattttggc tggagctgtt
     2521 caagggctac ctcgccttct acaaggcgac gatccccgaa gaggtgatag agctcacgtg
     2581 gcagcgcctc atcgccggtg agcccggctt ccacatcggc ctggtcgcga cgaaggagga
     2641 cgacgtgccg gtcggactgg cgcacgttct gttccaccgg tcgacctggt ctcccacctg
     2701 gtactgctat ctcgaagacc tgtttgtcga tccgtccgaa cgcactaagg gcatcggacg
     2761 ggcgctgatc gaggccgtct acaaggaggc cgacgcgcgc cagtgcacga gaacatattg
     2821 ggcgacgcag gaattcaact accgcgcgcg ggcactttac gatcagctcg cgacgaagtc
     2881 gccgttcatc cagtatcggc ggtgaagata tagcgaacag gagcgacgat gcagcagact
     2941 gacaggctat ggttgctcga cacccaggtc aggattttgg tttcgtgcga tgacgaatcc
     3001 cacgggatca cgatcatcga gcacgaggcg ccgttcggcg attcgccacc gctgcatcgg
     3061 cacctacgtg aggacgagag ctttcacgtc atcagcggca cgctcgacat cgtgaccgat
     3121 ggagagatga agaggctcgg ccccggccag actttgcgcg cccccaaggg gggcagccac
     3181 acctacaggg tggtttccag cgacggggcg cggttcctca ccatcagcgg cggccgggat
     3241 ttcgagcagt tcgtcagaag aatgggccga gtgccgaccg gtccgggact gccagagcgc
     3301 tcaggcccgc cgagcgagga ggccgtcgca gagctcgtcc gcatttgcgc ggagcacggc
     3361 atcgaggtcg tcggtccgcc gctgacctga cccgggcact gccgggcgtc attggcggat
     3421 caactgctcg gtgcgtcgag cctgcgggtc aggttctcga actccttgcg cagctcctca
     3481 ttgcggaaga tctgctcgaa cgtcggggcc tcgagcttct ggatcgcctc gaacgaggcc
     3541 gagctgtcgc gcatcagatt gcggagctgg aagctcgctt cgacggtctc gtaggtattg
     3601 tccgcgatgc gcaggtcgtg cgcggcgcgc cggcgggcct cgctgatctg ctggcgctgc
     3661 tgcaacagat aacgccggta acccttggcc gcttcggccg ccagtttctg gctttcgcgg
     3721 ttggcttcga gcgcgcgccg ctggtcgggg cgattttcgg ccttgagcaa ctccatcgtc
     3781 ttgcgccgcg ccacctcgat gtccttcgcg atctcgtcga gcttcggcag ataattgtcg
     3841 tcgatcttgg cgatcagcgt gtcctgggcg tggacgagca tggcgaacag cgcggcgtgc
     3901 atggcgaaat acttgcgggc cgcgttcatg ttgtcaccgg acgcggagag cagcttcgca
     3961 agctgacgat cgatcagctt ggcggaattg aaaaccgccg tcagccgcac gaggtcaccg
     4021 gccagcacgc tgtcgagcag cagatcgacc tggtccgctg acagctcgac gcctgaagcg
     4081 gcgagcgcct ggtgaatctc ggtctttgca gcctcgatgg ccttgcggtt ttcctcgatc
     4141 ctgcgctttg cctcctcgat ctcgcgaccg aggctgtcga cggtatcagt cagtacaccc
     4201 ggcagcagcg agtcctctgg cgcgacgagc tgcttctcct tcagcttgac gatgcggtcc
     4261 tcgaggtcac ggatgttctt gcgtagtgtc tcgacccggc gctgaatgtc caccacgggc
     4321 acgtcagtga cgatcccgag cgcggcatcg agcaagtcgc ggatcttgcg ctcgcggtcc
     4381 tcgcgggttt ccgtccacag tggagtgacg acgaattcgc tcctggaggg gagcttgcgc
     4441 gcctcggtgc ggttgcgggc ggcatcctcc agcacggcgt cgagggcacg catcagcttg
     4501 cgcgcctgct cgtaggtggc ctctccttcg cgtgcatccc tctcggctgc ctgggtttgc
     4561 gccgcgtttg gctgcccatc aggctgcccc tcgcgcgcca gcagctcact gatgccgtcg
     4621 cgacggctgg tctgcgccga cgcatcgagc gtgcccagta cgactgctcc cgcggtcagc
     4681 gccaaggcca taacggcagc ctgagacgtg gtcaccatcg tcgtacctcc acctacaacg
     4741 cgaatggtac caaattcgag cgaatccgtg aagaaacgct ctcccccgaa acgatcaggt
     4801 gattacgagg ggcgctgatt gcaatggctg tgcgcggaaa gcctgcgtgt gtaagcctgc
     4861 ctgcttcgtc caaatccgcg agaaccgtgt cgaggctggc caggcgctgg cctcccgtgt
     4921 catgtttcgg cgctggttca ggttggcaga gagagatggt ttcatgagca cattcgagcc
     4981 cctcgatcct gattacgagg cgcgtgtacg ctcgagcttt gcgagacaga gatttatgcg
     5041 ctttctcgga gccgagattc tgcggatcgc tccgggtgag gtggatctcg cccttccgag
     5101 ccgggagaac ctcacccagc agcacggcta tttccatgca ggggcgactg cggcgatcgc
     5161 cgatacggcg gcaggctatg cagcgctttc cctttatccg aaagggacag gtgtgctgac
     5221 gacggagttc aagatcaacc tgctacggcc ggcgcaaggc cgccggctca tcgcgcgtgg
     5281 gcgggtgttg aagccgggcc gcacgctcag catctgtcag tccgatgtgt acggtgagcg
     5341 tgacggccgc gagacgcacg tggcgacact gctgctcacg atgatgtgcc tcgaagggct
     5401 gcaggactga agatcagatc agccgccgtg tgcgcgcctt gtaagcccga tacgcatccc
     5461 cgaagcgcgc ttccaggtgc cgctcttccg ggatgatagc cagccaggtg acgagaatcc
     5521 cgaacaccgg ggtcagcagc accagccata tgttgcgcat caactcggcg atgccgagca
     5581 gaatgagcat gtcggcaaga tagattgggt tgcggcgaaa gcggaacggc ccgtcggtca
     5641 ccagtgtcga ggcggcttcc gtcggcatga ccgtcgtgcg ggcgcggcga agcgtgatga
     5701 tcgaccaggc catcagcgct atgcccgcca ccccaatggc caaaccggcg atgtgggaag
     5761 ccgtatcgtc cattccgggc caggagagtg ggaccagcag ttggaggacc caggccgcta
     5821 ccacggtcag gacgagcagg accggtggcc acgggaacgg gctcggccgg tctgccgatg
     5881 aatggtgctc cgtctggctc atcgcggctc cctgacaagc ttcggctgac attctatagt
     5941 ccgcccgatc gtcgagttgg gcaatggctg cgactccccg accgtcatga ccgcggaggc
     6001 ggggcatgat gcgcgtccgc aggcaatccg tgtgcttgcg gcgaggcgcc aggaaggcga
     6061 tggcggaagg ggagtgcccg ggcccaattc gacaagtccc gatgcaacgt gaccgccaag
     6121 tcgcccggag ctgccatgat cccgaatgcc ctgccgtcct tcaacttcga cctcggcgat
     6181 accgccgacc agctgcgcga gacggtgcgc acgttcacgt ccgacgaaat tgctcccatc
     6241 gcggccgaaa tcgatcgcac cgacgagttc ccgcgccacc tctggccgaa gatgggcgcg
     6301 ctcgggctgc atggcatcac ggtggaggag gagttcggcg gcgtcgggct cggatatctc
     6361 gagcatgtcg tcgccatgga ggaggtgagc cgcgggtcgg ctgcggtggg cctgtcctac
     6421 ggcgcgcact ccaatctctg tgtgaaccag atccgccgca atggtacgcc tgagcagaag
     6481 cggcgctacc tgcccaagct gatctcgggc gagcatgttg gcgggctcgc catgtccgag
     6541 cccggcgccg gctcggatgt cgtctcgatg aagcttcggg ccgagaagaa gggcggccgc
     6601 tacatcctga acggcaccaa gatgtggatc accaacggtc cggggggcga tgtcatcatc
     6661 gtctatgcca agaccgatcc ggacgccggc ccgcggggga tcaccgcctt tatcgtcgag
     6721 aagaatttca aaggcttccg cgtcgcccag aagctcgaca agctcgggat gcgcggctcg
     6781 aacaccggcg agctcgtctt cgaagactgc gaggtgccgg aagagaacgt gctcggcacc
     6841 gtcaacaagg gcgtcaacgt gctgatgagc gggctcgatt acgagcgcgc ggtgcttgcc
     6901 gccggaccgc tcggcatcat gcaggcgtgc atggacgtgg tgattcctta cgtgcacgaa
     6961 cgcaagcagt tcggcgcacc gatcggcacg ttccagctcg tgcagggcaa gatcgcggac
     7021 atgtacgtca cgctgaactc ctgccgggcg tacgtgtacg cggtggcgag ggcctgcgac
     7081 cgtggagaga ccacccgcga ggacgcggcg ggcgccatcc tgctcgctgc cgagaaggcc
     7141 actcaggtgg cgctcgatgc catccagctc ctcggtggca acggctacat caatgattac
     7201 ccgacgggcc ggctgctacg ggatgccaag ctctatgaga tcggcgccgg gaccagcgaa
     7261 atccgccgga tgctcatagg gcgggagctg ttcgaaaaga cggcgtgaga tcagcaactc
     7321 gtgaccggcc tgcaacggtc cgcgcaatca ttgcgcctac atctcagcgc agtcgcactt
     7381 tcgcctgaat cgaccacgta actgccgacc agccgacaac ggacggctta cgacctaaca
     7441 cacggtgata gggggcggga tatgaggttc aaggcggccg ctgcagctct gctcgcgcca
     7501 gctctgctga tgggctgcgg gccggatggt ccgacaaagg ccgatacggg gctggctgtc
     7561 ggtgccatcg ctggcggcat tctcgggaat caggtcggca aaggctcggg ccgcgtgctg
     7621 gcgaccgtcg ccggcgccgt ggtcggcggc atcgtcggca gcgagatcgg gcgggcgctc
     7681 gaccgtcagg accagatgct cgcccaggaa gcagagttcg aggcgctgga aagagggcgc
     7741 tcgggtgagc gtaggcgctg gcggaatccc gagagcggcc gctatggcga agtcattccg
     7801 ggtgagcgtt atcgccgcgg caccgccgac tgccgggact ttacacacac gatctatatc
     7861 aacgggcggc cggagaccat gcggggaaca gcgtgccgca atcccgacgg aacctggcag
     7921 aacgtcggct gagctcgatc cgcctgacgg cgggacagat ccggcctgag ctcaaaacgc
     7981 tgctccgcga catcggggca gccgcgcggc ccttgccagc ctgggggtag gggcctattc
     8041 cccttcgtag gaaaaaacct gggcgcgctg atgctatttt gggtgctctg cgctgttctg
     8101 acggccgcgg tgctctattt cgtgacgcgg ccgctgacgg tgcctgtcgc tggaccttcg
     8161 gcgcttgagc gcgatagggc ttccgacctc gctgtctacc gcgatcaact gagcgaagtc
     8221 gaggccgatc gcgcgcgcgg tctgctgtcc gatgcggagg cggaagccgc ccgcatcgag
     8281 atttcgcgcc ggctgctggc acgagccgag tccggcgagc ccgcgcaggg aggatctggg
     8341 gagcgagggc tgacgggcgc cagaatggcg ttcatcacgg ccgttattct ggtgccggcg
     8401 gtgtctctgg ccctctacct ggcctatggc tcgccgggcc tgccgggacg tcctcacgcc
     8461 gagcgggttg ccgcgtcggc agacaaggcg acgatcgaga ctctcgtcgc caaggtcgag
     8521 gagcgactga gagcgcaccc cgaagacggg caggggtggg atgtgatcgc ccctgtttat
     8581 ttccggcagg gccggttcga cgaggccgtg caggcctatc ggaacgccat caggcttctg
     8641 ggtgaatcgc caaagcggct cgccggcttg ggcgaggcgc aggtcttcgc gaagaacggc
     8701 atcgtgaccg aggatgcgag gcgggccttc cagcggcttg ccgagcttga gccgtcgcgg
     8761 ccggagcccc ggttctggct cgcgctcgcc aaagagcagg atggcgatat cggcggtgcg
     8821 ctcgcggact atcgcaagct cgtcgcggat gccccggcgg atgcggactg gcgcgctccc
     8881 gtcgagcagc gtatcgccat gctgacgcag aggcgcgctg gcgatcctag ggatgaagcg
     8941 gagcgtggtc cgaccgagca ggatatcgcg gcagccggga agctcgcgcc cggcgagcgg
     9001 gccgcgatga tcgagcagat ggtcgaagga ctcgcccaac gcctcgaaac cgaaagtcag
     9061 gacctggccg gctggcagcg cctcatgcgc gcctatgccg tgctcggaca gaaggacaag
     9121 gccaacgacg cgctggcaaa agcacgcaag gccttcgccg gggatgagac ctcgctcgct
     9181 gcgctcaacg atctggcgcg caagctgggc ctcgactcat gaggagcaag tgaggcgtcg
     9241 atgacgcgga agcaaaggcg cgccattctg atccttgcct gcgtgggcct gctcgggctt
     9301 gcgagcatcc tcgtcctctt cgctttgcgc gattcgatcg tcttcttcca tacgccaagc
     9361 gaagttgcag agaagggtat cgaggccggc cagcggttca ggctgggcgg tctcgttgca
     9421 gaaggatcgg tgcgccgcgg ggagggcgct gcagtcgagt tcgctgtcac ggacacggtt
     9481 aagaccataa gtgtcgtcta ccgtggcgtt cttccggacc ttttccgtga agggcagggc
     9541 gtggtgaccg aaggccggct cgatcctagc ggcaagttcc atgctgatac ggtgctcgcc
     9601 aagcacgacg agaactatat gccgccggag gtggcaggtg cgctgaaaga caagggtgtc
     9661 tggcagcaca tgggcaaaca acagccggcc acgaactgag gacggaccgc accattgatc
     9721 gttgagatcg gacattttgc gctcatcctg gcactggctg tcgccctcat ccaggcgacg
     9781 atgccgcttt ggggcgccca cattcgcgac gaacgcctga tggctgtcgg cccgcccgcg
     9841 gctctcgcgc agttgctgct ggtcgccatc gccttcctag cgctgatgta tgcttacgtg
     9901 tggtccgatt tctcggtcga gaacgtctat ctcaactccc attccgccaa gccgctgatc
     9961 taccggatct cgggtctctg ggggaaccac gaaggctcga tgctgctgtg gatcctgacg
    10021 ctctccctgt tcggcgccgc ggttgcagtc ttcggcggca aacttccgga aactctgaga
    10081 gccaacgtgc tcggcgtgca cggtgcgatc gccgtcacgt tcattttgtt catcgtgctg
    10141 acgtcgaacc cgttcaagcg cctcgtccct gcgcccgtgg aggggctcgg cctcaatccc
    10201 atcctgcagg acccggcgct cgccttccat ccgccgttcc tttatgcggg ctacgtcggc
    10261 ttctccatcg ccttcgcatt tgccatcgcc gccctgatcg aaggccggat cgacgccgcc
    10321 tgggcccgct gggtgcggcc atggactctc gctgcctgga tgatgctgac gctcggcatc
    10381 tccatggggt cgtggtgggc ctactacgag ctcggctggg gcggctggtg gttctgggac
    10441 ccggtcgaga acgcctcctt catgccatgg ctcgccggca ccgcgctgct gcactcggcc
    10501 gtcgtgatgg agaagcgcga ggcgctcaag gtctggacga tactgctggc catcctgacc
    10561 ttctcgctga gcctgatggg cacgttcctc gtgcgttcgg gcgtgctgac ctcggtgcac
    10621 gcgttcgccg tcgatccggc gcggggcgtg ttcatattgg gcattctcgt catcttcatc
    10681 ggcggggcgc tcgcgctgtt cgccctgcgg gtcggggagc tgcggcaagg cggcttgttc
    10741 gcgccgatca gccgcgaagg aagcctggtg ctcaacaacc tgctgctgac gacggcctgc
    10801 gccaccgtct tcatcggcac gctctatccg ctcgccctcg aagcgtttac cggcgacaaa
    10861 atctccgtag gggcaccctt tttcgacgcg accttcgggc cgctgatgct gccattgctg
    10921 gtggcgctgc cgttcggccc ctatctggca tggaaacgcg gtgacctgac ggcggccacg
    10981 cagcggcttt gggcagcggc agccgttact ttgctcgcga tcattgtggc ttatgccgtc
    11041 gcctatcctg gcccatggct ggcgccgttg ggcattgctc tcggcgtttg ggtgatggcg
    11101 ggtgccttga gcgagtgcgc ctcgcggatc aagctggggc aggtgccatt ggcggaaagc
    11161 tggcggcgtc tcacgggcct gccgcgcgcc ggcttcggga tgtccgttgc gcacttcgga
    11221 gttggcatga tggtggtggg tatcatcgcc accagcgcct accagagcga gcgcattctg
    11281 gtgatgaagc caggggacca ggtggaaatt gcgggctaca cgctgacctt caaaggagca
    11341 gcgccgcagc gcgggccgaa ctatcgcgag gaagtcggat tgttcgaggt ggagcgcggc
    11401 ggccgtgtcg tgaccaatct cacgccatcg aagcggatgt acgacgcgcc cccgcagccg
    11461 acgagcgaag ccggcatcca cgtctcgtgg cgcggggacc tttatgtcgt gctgggagat
    11521 caacaaacgg actcctctgg cggatatgcg gtgcggctct atttcaaccc gctcgtccgg
    11581 ttcatctgga ccggcgccgt cgtgatgttc ctgggcggcc tgttgtcgct ttcggatcgc
    11641 cgcctgcgcg tcggtgcgcc gaggcgggcg cgccgcgtac gcgccgcagc tgttcctgcg
    11701 gagtgagatg atgatggcgc gtccgtgccg ggccgtcctg tggctcctcg ttttcccgtt
    11761 tctgtttctc ggcgcggccg cctcgctcgc cgtggagccg gacgagatgc tcgccgaccc
    11821 ggctctcgag gcccgggcgc gcgcactgtc ggccgagctg cggtgcgtgg tgtgtcagaa
    11881 ccagtcgatc gacgattcga atgcgccgct ggcacgcgat ctccgcattc tgctgcggga
    11941 gcgtctagcg gccggagaca gcgacgagca ggtgaagcag tttctggtcg accgctacgg
    12001 cgccttcgtc ctgctcaggc cgccgttcaa cgcgcacacg ctcctcctgt ggctcgcgcc
    12061 gccgatcatc ctcgtaggtg cgggagtgct cctctttcgc tacatgtcgg gcagaggccg
    12121 caccgcaccg gccgaggtga gccagtcttt gacggccgag gaagaagaga agctgcgctc
    12181 gctgctggcc gccgagagcc ccgatgcggg ctccgccggg cgcgaaaacc gctgacgccc
    12241 gtcgttctgc cggcgaaaac gagccaacct taccgatatt taatcctcca gaaacgccgc
    12301 tgtaaggcgc ggtgacctac atttgcgggc aagtcccatc agcgacttgg aaaagaggag
    12361 cctcatatgt cgaccgagaa cgacaaagtc cgtgcaacgc gcattcggat gccgcgtcgc
    12421 acgaaggggc cgcttgtggc ggcgctcgcc ggtgtcagcg cgcttgccct ggtgaccgtg
    12481 tcaccgtcaa ttcctgtgcg ggcgcagttc aacccgcaag gctcgggcgt tgagacgctc
    12541 ggacgggctc cgctctcctt cgccgatatc gtggagcgcg tgaagccggc ggtagtcagc
    12601 atcaatgtca tcgaagggga agcgcgggtt gcgcgcaatg acgacgacaa aaacctgccc
    12661 gacggtttcc caaacctgcc cgaggatcac ccgctgcacg acttcttcaa gaacctgccg
    12721 cgcgaattcc gcatgccgat gccgcgtccg atgccacgtc agcggcaggc aaccgggtcc
    12781 ggcttcctca tctcgcccga cggctacgtg gtgacgaaca accacgtggt cgacggtgcc
    12841 accaaggtca ccgtctcctt cgaggagcag gagaaatacg aggccgacat cgtcggcacc
    12901 gacccgcgca cggatctcgc ccttctcaaa ataaaggtgg ggaacggcaa gcaattccca
    12961 tacgtgaagt tcgcgtcgaa accgccccgc gtcggtgact gggtgctcgc tgtcggcaac
    13021 ccgttcggcc tcggtggcac ggtgacggcg ggtatcgtct cggcgctcgc gcgtgacatc
    13081 ggctcgggcc cgtatgactt catgcagatc gacgcggccg tgaaccgcgg caactcggga
    13141 ggcccgacat tcaacctcaa cggcgaggtc gtcggcgtca acacggcgat ctacagcccg
    13201 tctggcggca acgtcggcat cgccttcgcg gtgcctgccg agacggcaag cgaggtcgtg
    13261 acgcagctca aggagcgggg gaccgtccgc cgcggctggc ttggcgtgcg tatccagtcc
    13321 atcaacgagg acatcgccgc aggactgggc ctcaaggagc cgaagggtgc actcgtcaac
    13381 gacgtgacgc cgaatggccc ggcagatcaa gctggcctca agagccagga tgcgatcctg
    13441 gccgtgaacg gcgacccgat cagcgacagc cgtgagctgg cgcgcaagat cgccgagttc
    13501 gcgccaaata cgccggtgga catcaagatc cttcgcaaca acaaggagat gaacgtcaag
    13561 gtgacgctcg gcaccttccc cgacagcaag gaggagatcg cgcggctcga gcagggtgga
    13621 ccggcagggt cggagatgaa cgccctcggc ctgacgctcg gcccggcccc ggcccggaag
    13681 ggcggcaaaa tcgaaggcgt ccagatcacc gatgtcgatt cggactccga tgcagcggcc
    13741 aaggggctga agtccggcga tgtgatcctc gaggtgaaca gccgcgaagt gaagtcgccg
    13801 gccgacgtcg aggaggccgt gaaggaggcc aaagagctca accgcaaggc cgtactgctg
    13861 accgtccgct ctggtgacca gaaacgcgtc gttgccgtcc agatgaaggg ctgaagtcaa
    13921 ccagcctgac gtgacttgag cgaggcggcc ccgtccggga tttctggaca aggccgcctc
    13981 gttccgtttc tctggatccg atcgcgaagg caggctccag atgcgcgtgc tggtaatcga
    14041 agacgatcga gaagcggcgc acttcctcca gaaggcgctg aaggagagcg ggcatgccgc
    14101 cgtcgtcacc cacgacggtg aggaagggct cgaggccgcg cgcgagggca agttcgatgt
    14161 gctgatcgtg gaccgcatgc tgccgaagct cgacgggctg tcggtggttc gtctgctgcg
    14221 ggacgagggc gaccgcacgc cggttctgat cctctccgca ctgggggagg tcgacgacaa
    14281 ggtcaagggt ctgcgggccg gcggggacga ctatctcacg aagccgtatg ccttctccga
    14341 gctgctggcg cgcatcgagg cgctgtcccg gcgcagcgat ccgcagcagg agcagacccg
    14401 ctacgtcgtc ggcgacctgg tgctcgaccg gctctctcat cgcgtgaccc gggccggaga
    14461 gccgattacg ttgcagccgc gggagttccg cttgctcgaa tacctgatgc gccatgccgg
    14521 tcaggtcgtg acgcgcacca tgctgctcga gcacgtctgg gattatcatt tcgatccgca
    14581 gaccaatgtc atcgacgtcc acatctccag gctcagagcg aagatcgaca agaacttcga
    14641 ggagtccctg ctgcatacga tccgtggcgc gggatacatg atccgtgctg gcaagagcta
    14701 acccgctgct gccgcatttc acccggacga cggctttccg gctgatggcc gcgtacgtgg
    14761 ccgttttcgt gaccgtcgcg gcggtgattg ccggggtgat cctctggcag acgaaccagc
    14821 tcctcatccg ccagatcttc gccacgctgc gcacggaagc cgaaagtgtg caggatgccg
    14881 tcaggcagcg cggcccggct gcggctccgg acatcattgc cgagcgcagc cgcttgtcgg
    14941 aaggaggcct ctacttcctc gccgatccgc agggccataa actcgccggc aatctcaacc
    15001 ggtggccgcc cgagctgcgc gaccagtcag gcgggttgtt ccgctacgca gcaacgacag
    15061 gggagcaatc gcgggagcgt gttgcgatcg gcattcccgt cgccttggag cgaggccagc
    15121 ggctgctcgt cgcccgcgac gtggaggagc agcgccagtt catcggccgg gtgcggtcgc
    15181 tttttctggc tggcttcgga cttctggtgt tgctggggct cggcgggggc ctggccgcga
    15241 gccgcctgat cctgcggcgg atcgcgaaga tcaccacgac ggctcggtcc atcatgcgcg
    15301 gcgacctttc ccgccggatc gcgaccagcg gcaccggaga cgagctggac gagctgtcgc
    15361 gcaatctcaa cgccatgctg gaccgcatcg aacagctcat ggcgggcctg aaagaggtct
    15421 ccgacaacat cgcccatgat ctcaagacac cgctcaatcg cctgcgcaac cgcgccgaag
    15481 cggcgttacg ggaggcccgt tcggcggagg attaccagga gggcctggag cggacgatcg
    15541 acgaggccga tgagatcatc aagacgttca acgcactcct gctgattgcg cggctcgagg
    15601 ccggcgccgt cgacgagagc aaggagatct tcgacttgac agcactggtg cgtgatctgg
    15661 tcgagctcta tgagccgcac gctgaagaaa accggcggaa gctcgcgttc gaggccgaac
    15721 aaggagaaat ccttgtgaga gcaaaccgac agctcatcgg ccaggcggtt gccaatttgc
    15781 tcgacaacgc catcaagtac ggcggaccgg ctcccgattc gccgaatggg ggctccgcga
    15841 gcgacatcga ggtccgcctc tcccggaccg agggcggcgt cgagatcgtc gtggccgatc
    15901 acggcccggg cattcctgcc ctcgaccggg agcgcgcgct gaaacgcttc gtccggctcg
    15961 aggcgagccg cacgcggccg gggacagggc tcggcttgag cctcgtggcg gcggtggcgc
    16021 ggctgcatga gggccaagtg aagctggagg acaacaagcc gggattgcgg gtcgtgcttt
    16081 cctggccgat cccggcgatg ctggagagac ccgagacgaa ggtggcggtc agccagacga
    16141 ggattgccca atgagcacac tggcaagcgc gccagcttct cttcaagacc gtttgcgaca
    16201 ggccccattc ggcgcggagg acccgcacat cgcggaagcc gttcgcgagc ttgaggcgga
    16261 gatggcggac gcttcgcgcg ccgccggttc cagcatgacc gagcaggccc ggcaactcct
    16321 gggcggcgtt ctcgccggct cgccatacct ttcaggtctg atgcgccgcg atccggcaag
    16381 gctcgtccgg gtcctctcgc gcgctccgga ggaaagccgc gctgatctgc tcgaaagcgt
    16441 ggctcgacag gcgacggcag cgcagtcagt cacggagctg atggccgccc tgcgcatctt
    16501 caagaacgag gtcgcgctgc tgacggcgct tgctgatttg ggcggcgtct gggacctggc
    16561 ggatgtcacc cgcacgctca cacttgccgc ggatgcggcc gtcgagcacg ccgttcgctt
    16621 cctgttccgg cgggcagtcg ccaatgggac ctgggcggcg ccggaccatg ccgccccgga
    16681 ccgggactcc ggctacatcg tgctcggcat gggcaagtac ggtgcccaag agctcaatta
    16741 ttccagcgac atcgacctca tcgtcttttt cgacaagcag cgcctgaagc tccgcgccga
    16801 cctcgagccg caaaccttct tcgtgcgcat gacacgcgag ctggtgcgta tcctgcagga
    16861 gcgcaccggc gacggctacg tcttccgaac ggacctcagg ctgcgacccg accccggagc
    16921 gacccaggtc gcgctgtcga cggacgcggc gctgacctac tacgagagct tcggccagaa
    16981 ctgggagcgc gccgccatga tcaaggcgcg gccggtggcg ggggacatcg cagcgggcag
    17041 agcgctgctt tcggagctgt cgccgttcat ctggcgcaag tatctcgact tcgccgccat
    17101 cgccgacatc cacgccatga agcgccagat ccacgcgcac cggggcttcg gcaagatcgg
    17161 cgtggccggg cacaacatca agctcggccg cggcggcatc cgggagatcg agttcttcgt
    17221 acagacgcag caactcatcg ccggaggtcg gcagcccgat ctcaggacgc cgcaaacgct
    17281 cgtcaccctc gatcgcctgg cggagcgcgg ctggatcaag cccggggttc gtgacgacct
    17341 ggcgcgcagc tacacctatc tgcgcggcat cgagcaccgc ctgcagatgg tggctgacga
    17401 gcagacgcat tcgctgccca atgcacctga agcgctcgcc tcgttcgccc ggttcgcagg
    17461 ctatccatcc acggacgcgt tctcgaaaga gctggtcgcg catctggagg cggtgcagcg
    17521 gcactacgcg gctctgttcg aggacatgcc ggagctgacc cgcggcggcg ccaatatggt
    17581 gttcgcgggc tcggcggacg acccgcagac ggtggaggcc ctggccagga tgggctactc
    17641 gcagccgcat caggtgatcg acaccgtgcg cggctggcac cacggccgct atgccgccgt
    17701 gcgcacggcc caagcgcgcg agcggctgac ggaagtgcaa gcggtgctga ttgaggcgct
    17761 ggcgcaaacg gccaacccgg acagcgcgct ggccagcttc gaccgattcc tggcccaact
    17821 gccggccggt gtgcagctgt tttcgctgct cagggccaat cccaacctgc tgcggctggt
    17881 ggcagacatc atgggctcgg cgccgcggct ggcgcgcatc ctgagccgcc gccggcgggt
    17941 gctcgatgcc gtgctggacc cgcgcatcat cggtaatctt ccaactccag aggagctcga
    18001 tgcgctgatc cgcgacgaga tcgagagcac ggacgactat caggagatcc tggaccgcgc
    18061 ccggatcatc ggcaacgagc agatgttcct gatcggggtg cgagtacttt ccggcatcat
    18121 cgtcccgtcc caggccggcg gagcctacgc gctgctcgcc gaccacctca tccgggcgct
    18181 gctcaaggcg gtggagcggg acttcgccag gttgcacggg cgggtttccg gcggccaggc
    18241 ggcggtcgtg gccatgggaa agcttggcgg ccgggaaatg acggccgcct ccgacctcga
    18301 cctcatactc gtttacgatt tcgcgccgga tgcggtgcag tcggacggca gccgaccgct
    18361 ggcgcccagc cagtactacg cccgcctgac ccagcggctg ataagtgcgc tttcctcgcc
    18421 gacggccgaa ggacagcttt atcaggtcga catgcgtctg cgtccttccg gccagcaagg
    18481 cccggtcgcc acgcagcttt caagcttcat cgagtatcag gcgcaccaag cctggacctg
    18541 ggagcacatg gcgctgaccc gcgcacgtgt cgtcgcgggg gcgccggagc tgcgggccaa
    18601 ggtggagcag gccatccgcg atgccctcgt ccagccccga gaccgcgcga agatcgcagc
    18661 cgatgtgcac gatatgcgcc agcgcatcgc cgccgagaag ggcacggacg aaatctggga
    18721 tctgaagcag gtgcgcggcg ggctcgtgga tctcgagttc atggcccagt acctgcagct
    18781 cattcatgcg gccgagcatc ccgatgttct cgaccagaac accgttcggg ccctgcagaa
    18841 gctcgatggc aagggcctga tcccaggtgg cgcaggcacg atcctgattc cggcagccca
    18901 gctcatcaac gacctgacgc agatcgtccg tctgtgtgtc gacggtccat tccgggccaa
    18961 cgaggcctcc caaggactca aggacctgct ggcccgcgcg gcgcacgcgc cgagcttcga
    19021 gcgcctcgag gccgatctgc ggctgatgct cgccgagacg gcgagcctgt tcgatcaaat
    19081 cgtctcctga accggacgcg acgggttcgc agtggatatc tcgttcctgg attgccgggg
    19141 acgagatccc gacctcttgg agatgagagt atgaaaaaga agacgatgat cttgatcgcg
    19201 ggcgtgttga tcgccgcggg cggagctgcc gccgtggctg gcgtcggcga gaagcgcgct
    19261 cgctttggtc atgggccggg cacgggcgcc atgttcgcgc acatgggcgg gcatggattt
    19321 ggcttcggcc ggggcggggg attgaaggcc ctcgatgccg acggcgacgg cgtcattacg
    19381 atcgaggaag ccctggctgc tcgcagcccc gtatttgccc gtatcgacac caacggcgac
    19441 ggcgtgatcg acgcacagga gatcgaggcc gaggtgaacc tcaacaccca gtactggacc
    19501 caggcgatgt ttcaccggcg cgaccgcgat ggtgacggcc tgatcagcaa ggaggagttc
    19561 agcagcaaaa agtaccgtag cgagcggcgg gccgagcgtg ccgaggacgg caaggacgac
    19621 gatcgcagtg atcgcgcccg gcgcggccac cgttacggct ggcatggaca cggtcaccat
    19681 caccatcgct ggcatcacag ccgctggcgt ggggatagga tggcttcgcg cttcgagaag
    19741 atggacctca atgcgaacgg cacgctcgaa gccagcgagg tcgagaccgc gatcaagccc
    19801 cgtgtgacgc gccgggtgaa caggatgatc tctcggttcg atcaggacaa cgacggccgc
    19861 gtcacccgag aggagttcga gaagcccgcg cgcgatcgct tcgcgatgcg ggacatcaac
    19921 aaggatggca ggatcagcga ggaagacctg ccgccgatga tgcgcgggcg gggcattctg
    19981 cgctagtgtg atgatcgaga aattcgcagg cggtcgcgac atgtgtctca agcgaatttc
    20041 tcgatctgag tcacacgcgc gggtctatga ccctcgtgtc cctcttgatt gcgaagttcg
    20101 ctcccgaacc tggtcgcgaa ctccagcgag cttcggaatc gggacaccag aacgggcgca
    20161 cccgatgacg tacggggagg gacggtgcga ggaaagtggg gtgagcaggg gggatcttcg
    20221 gccgggtctg ttggcgggcc agccggggac tccacgacca ctgacgcctc actcctcgcg
    20281 cgcgcgggac gcggcgatgc cgccgctttc cgcgagctgg tggaccggca cctccccgca
    20341 gccctgggca tcgcaaggcg catgctccag gacgatgcgg aggccgagga cgtggcgcaa
    20401 gaggcgctgc tgcgcctctg gcggacggca tctgacctgg aggtcggtcc tgcgggagtc
    20461 cggccgtggc tcaggcgagt ggtttcgaac ctgtgcatcg accggatccg ctcgtcgcaa
    20521 cgcaccgatg tgactgacga ggtcccggac atgccggcgg agacgaacca gtttcgcgag
    20581 ctgacccagc aggacctgtc ggctcgcgtc gacgaagcgc tgaagaactt gccggaacgg
    20641 cagcgccttg cgctgacact gttccactac gagggcatga gccatctcga gatcggagag
    20701 gtgatgggcg tgtcggtcga ggcggtcgag tcccttctgg gccgggcccg ccggacactg
    20761 aaggcgacgt tgaagaatga ttggcggcag ctcctctcgg aggcctgagg gcagtcgcgg
    20821 aggcggaaat ggcaggtgat aagaattcgg cagatttgac agccctcgag gagcacctcg
    20881 aaatccacgg ctctcgcatc gagcgttggc cggatgccgc ccgccgacgc ttcgatctcc
    20941 tgctcaagga gaacgcacgc gcgcgcgagc tgctggccga ggcgcgcgcg ctcgacgatt
    21001 tgctcgaccg ggcgcccctg ccggatgcgc gccgcatggc ggacctcact gagcggattg
    21061 ccgcgctcgc gaaggccgaa gggagagaac agcggacggc cgctcctgtg atcgacctca
    21121 cggcgcgccg ccaggcgcga cggccgccgg tcacgccgat gcgctggaaa gtcgcctcgg
    21181 cgctggcggc ctcgttgctc gccggcatct atctcggatc gtcgccgccg gtcagcaccg
    21241 ccgtcgagtc tgtggcgagc cttgtcggtg tcgcccctga caacgagagc tccgaccttg
    21301 tgctgatcta tgatcctgct gctgatgaag aggaattcct atgagcgagc ctgagggttc
    21361 aggggcggca tccgcacgcc gggtcccgcg ctggctgtgg gccgtgctca tcgtatccct
    21421 gggtctcaac ctgcttgtgg ctggggtggt tgcctcggcc gcttggcatt ttcacagcct
    21481 caatggctcg cgcgggcggc tttcggccta tctcgagacc ctgcctccgc agagatccga
    21541 atttttgcaa ggcatccggg agcgcttcca gccggtattg cggccattgc gccaggaaat
    21601 cagagagacg cggcgtgaag cagcccggct cttcgcggcc gaccctctgg ataagcaggc
    21661 tctcgtcgcg acgcatcagc gcctgatgga cgcggaggtt cgaattcgcc agtcctacac
    21721 ccagctcatg acggagctgg cggagaacat gacgccgcaa gagcgccgcg acttcataga
    21781 atggcgggag caacgccgcc gcggctcgca ccgctcagcc aacctcagca gtcagcagta
    21841 gcagcgcgct gcctgactgt tcccgcaccg caccctgccg ccattgcaga gcttcgtcta
    21901 gagtcggtcc gctgcccggc gttgctcgta ggccttcaga tgcgtgaagc tgatgcgatg
    21961 cagcgtgtcg tcgatgccgt gagcgcgggc cttctccagc atgaagccga cgatgtgatc
    22021 cgattcgacg ggacctttcc gctcgatgtc gcgcagcatc gaggcggcca tctgtgagcc
    22081 ggtgtcggtg aagaccttac cgaaccgttc gatgaattcc tcgggcatgg ggtggccttc
    22141 cgcggcggcg atctgcgcat tccgatcgag aaacgccagc gtcaggccgc tgccgtccgg
    22201 cgtgcgcgca atctcgccga cgttggcccg catcagcgtc gtcatgctgg cgagcgtcga
    22261 cagcatgacg agcttttccc acatcttctg gatgatgtcg ggaacggcga cggcgttcgc
    22321 cttcccgctg gtcttgtcga acgctgcctt gagcgcctcc acccgtgggc tcgaggcggc
    22381 gttttgttct ccgaatgtga tcgtgttcca gtcgttgagg tgcttcacga gcccgtccgg
    22441 ctgcaatgtg atggcaatct gtgccatgcc gccgagaacg cgctcccttc cgaagacctc
    22501 gttgagccgg tccatatggg acatgccgtt gagcagcgga aggatcgcgg tattcggccc
    22561 gacggccggt ctgatggcct cgattgcgct atcgaggtcg taggccttgc aggtcagcag
    22621 cacgtagtcg gcgggggagc gtatctcttc ggcgagccgc gcatcgacgg gcacggtgaa
    22681 atcaccgtgc acgctctcga ccctgagccc atccttttgg agctgcgcct tgcggccagg
    22741 ccgcacgaga aaggtgacgt tggccgccga ggcctccacc agccggcctc cgaaatagcc
    22801 accgatgcca ccggcgccga ggcatacaac gtgcatgact gtcctcccac tgatcccggg
    22861 tgctcaggcc aaacggcaac aaaaaaggcc tccgccaagc gaaggccctt tatgtaccag
    22921 ataaggcaaa acctcagccc tcgagcgact gattgatcca atcctcaaga cgctttttct
    22981 gcaccagcgc gccgacgtgc tgattggcga ccttgccgtc cttgaagagc atgagggtgg
    23041 gcatggcctg aattcggaac tgctgcgtca cttcagggct ccggtcgaca tcgaccttca
    23101 ccaccttgac cttgcctttc atttcctgcg cgacctgctc gagcgccgga gccatggcct
    23161 tgcacggccc gcaccactcg gcgaagaagt cgacgacaac cggctctggg gacttcagca
    23221 cctcggtggc gaagtcgcta tcggatacca tctgcgtcac ggtgaatctc cctgcagcgc
    23281 cccccgcgac tcgggaggcc aagcactttg tgtcgtccgg aacgtaagga gggggtcatc
    23341 gggcgtcaag ctgagggctg ccatgctccc agagcaaggc ctcgtgcaaa tccagaagct
    23401 cttgcggaat tggcatcagt cgcgccccgt ccgtccacag aatcgctgcg ctaacgggaa
    23461 gctccgggaa gacctgaccg acgacgaggc ggtaggccgc aagctgcagc agataggcct
    23521 tgggcacctc ctcgggctgc tgcggcgggg ggcggttggt cttgtaatcg agcagcaaaa
    23581 cctcgcccgg tagccggacg agcctgtcga tctgtcccgt gatcctgaca gcaggccgcc
    23641 ggcccgccgg gtcgggaatg gctgcggcaa ttggcacttc ggcgcggctt tccgggccga
    23701 acaagcttcc gaactgcgga tcacggagga tcgagagaac ttcctcgacg atgtcactac
    23761 ggacgttttc gggcaggctt tcgccgcgaa cagcaatgaa gctgtgggcg gcatcagccc
    23821 agcggtcgac cggaaaggat ggcagatgct ccagcagcgc gtgcgtgagc gtaccgcgca
    23881 ggaaccggta ttccgaggaa agagcaagcg gtgatggact gaccatcgag ggcgcagagg
    23941 ttcgagcgac gagcggatca ccgatctcgt ccgtttcgag gcgcgccaag cgcgatggcg
    24001 atagcgggaa cgtggtggcc ggctcacgcg gcgccttgcg ctttgcccaa tccggtagcg
    24061 gctcggggct cgcgagtctg gtggcttcgt tccgaggttc ttctggcggc gctgtctgcg
    24121 ggcaggcgta acgcagaacc gtcctgccgt cctccgtctc cacggtttcg cagacgtcgc
    24181 gcagtgcccc ctcgatcagg tcgtaccagc agcccttacc gcgcccgctc ttcgtctcga
    24241 aaccggcgac gtagagccgg tcgcgggcgc gcgtcatcgc cacgtagagc aagcggttgc
    24301 gctcctcggt ctccgcggcc tcgatcgccg ccttggcggc ctctactgcg gggtgatcac
    24361 gggtttcctt cactggccag acatagggat cgggaaaccc catcggtttg gtgacacccg
    24421 gaagcggtat gagcgcactg tgcggccggc ccgatcgcga cgagcaggtg tccggaagga
    24481 acacaatcgg ggcttccagg cccttcgctc cgtgcacggt catgacgcgc acttcttccc
    24541 gcccgtggtc ctggtcgcgc ttgatctcga cgtgcagccg ccgcagccag gtgacgaagc
    24601 cctgcagcga gggcgggttg cggtcgtcgt actggagcgc gagattcagg aactcgtcga
    24661 tcggctccgc cgcctcggcc ccgagccgcg cgagcatccg ctgacgcatg ccctcgttgt
    24721 cgagaatgcc tgcgaagaac tcgtaaggcg gccggtaatc ggcttcggcc cgccagcgcc
    24781 gcagcgtgtc ggcggcgaac tccagccgcg ggttcgtctt cgctgcggcg agaagcgcgt
    24841 tccagaggga gccgctccgg cccggcgcga aggtcagcag atcgtcgtcg tcgaggccga
    24901 acatcggcga cttcagaata gccgccagcg ccagatcgtc ctcgggcagt atcaggaact
    24961 cgccgagcgc gatcatgtcc tcaaccgcga tctgctcggt gagctgcatg cggtcgcttc
    25021 cggcaacagg aattccgcgc gccttcagcg ccgccaccat gggcgcggcg aacggctgac
    25081 gcttgcggac gaggatcaga atgtcgccgg gccggatggg ccgatcttcg gattggagcc
    25141 gctcgccgcg cgccagccag tccctgatcg tgttggcaat ccggtcggcg accttgatca
    25201 ccggcgactg cgccggcttg tcggcgagcg gtgaccaggg ctccgccggc tcgatcgttt
    25261 cgaacgcctc ggtcggccag atttccacga aaccggcctg tccgatgcgg cgcgcctgat
    25321 gacggacggg ctctgccgct gcggtgagcc cgggtgtgcg cgtgtgatcg gagaaaacgc
    25381 ggtccacgct ggccagtatc ggctctgtcg aacggaacga gagcgtcagc gggaccgtgc
    25441 gccagttgag acccgcctgc gctgcggtgt cctcgaagac ttttcccgtg acggcgaaca
    25501 gcttcggctc agcgccctga aagctgtaga tcgactgctt ctcgtcgccc acggcgaaca
    25561 gcgtgcgcgt cttctcatgg gcgccgaggc cggagaagaa ctcgcgggcg agccccgtga
    25621 tgatcgacca ctgcgacggg ctcgtgtcct gagcctcgtc gacaaggatg tgatcgagcc
    25681 cgttgtcgag cttgtagagc acccattggg cagagtcgga ggacttgagc aggctcgccg
    25741 tgtgccggat cagatcctcg aagtcgagag ccgcacggcg ggccttggcc gccgcataat
    25801 gctgccggac cgcgtccgcc agctgccaga gagcgagtgt cgcatcggcc agcttgtgtg
    25861 cgatccgctg cccgcagagc gcggcaaagc actgctgcgc cgcgatcagg gttgcatgga
    25921 ggtccggatg ggacgattgc aggcccttcg tcatcaggct ggcccgcatc tcgcctccgc
    25981 ccgtcaggaa atagcggcgc aattccgccg cccgctgctc cgcctcggtt cgcgccagcg
    26041 ccgcttcgag gatgccggcg tgcctggcgt ctgtcttcga tcccgtcgcc agcacggctg
    26101 ccgcatgcct tagatccggt tccggcagga cggtcgcgag ctgctgttcg acagccgaga
    26161 ggctcggcgt cgtgatgcca agggccgcgc tatagatctg cctggcttct tgcagcggat
    26221 cgccgtcgcg gaagcccagg cgggacatct cctcgagcca gtcgcggcgg ccaagcgcgt
    26281 cctggagcac cttttcgaag ctgtcctcgg ccgcgtaagc gacgacagtc tccagggcct
    26341 tcccgagctg ctcgtttccc ggcgacgtcg ccgtcctcag cacggcgtcg atcgactggc
    26401 gcagcagagt gccagccatc tcgtcgtcga gaatggtgaa ccccggcggg acgccggctt
    26461 cgagtgggaa gcgctgtaga aggcgctcgc agaacgcgtg gatcgtctgg accttcaggc
    26521 cgcctggcgt gtcaacggct tcggcgaaga gctggcgcgc gccgcgcacc tcctccgcag
    26581 acggcgaacg gcccaggaga ccggacagcg tcttgcgcaa ttcgggctcc gggcacgtgg
    26641 cccagtgcga aagctcatca aagacgcgtt gcgccatctc ggcggcggct gtcttggtga
    26701 atgtcaaaca gaggatcttg tccggagcgg tgccggagag cagcagcttc agcacgcgca
    26761 tcttcagcac gtgcgtcttg ccggcgcccg cattcgcgct gacccaggcg gaggtgtcgg
    26821 gctcggacgc gctgcgctgg gcgcgcgccg tttcctcgag cgccttcatg gccgcgtcag
    26881 tcatcgccgc ccccctccgc ctcggcacca agccactcgg ccacccgggc gagatgcttg
    26941 tagtcgtcgt aacggtagtc gtaggccggt ctccgcacgg cccggtaggg ggtcacgggg
    27001 tcgtcataga cggcgatcaa gcgttcgagg cctgctcgcg tctcgtcaat cagcgacgcg
    27061 atctcagcgt tgatggattg ctcgacaccg gcaggctccg cgcccgaagc ggagatgtag
    27121 cgcagcattg cgattgccga tggaccgagc ccttcgaagc cgcccgccag cgcgatgacg
    27181 gcctccagcg gcagctgcgg cgaccgtccg tctgcggcgc gcttggccag ggtcttcacc
    27241 tggctttcgc cggcggtctt gtagtcggtg atcacgagcc cgttttcggt cacgtcgatg
    27301 cgatcggccc gtgcggtgag cgtgaaggga cctgccggac cacttagtac aagagagccg
    27361 gacacctccg ccagtacgcg gatcacaccg gcccgccggt tgggctcggt ggccgcaaac
    27421 cagcgcgcga accgctcgag ccgcggaacc cagaatgccg ccacccgcgg attgcccgtg
    27481 tactcggtga gaatcttgcc ggcgatggcc atcagctcgc gcgccggttc gtccggcaaa
    27541 tccgcggggt agcatgccgc aaaccgcccc agggcctcgt gcacgatgcc gccgcgcagg
    27601 gcggcgctcg gctcggcgcc caagagaggc agcggctcaa gccccagcac gcgtttggcg
    27661 aagagcgcat agggattggt gatccagcgc tccacatcac tcaccgaaag ccgccgtggc
    27721 cgcagcgcca gttgcggaca agggcagggc ggacgtagtc gcgggatcgg ctctacgagg
    27781 tccctggccc gggcccagcc gagccacggt cggtcaggtg tgagcgcatc gctcaggccc
    27841 atgccttcga gcaatgcctt gatccgcagc agccaccggg acggcaccgt cggggcgccg
    27901 tcgaccttct ccgcacgggt gagatagacg gtgcgggcgc ccagaagctg tgcgaaatcg
    27961 tgagcctgat ctccaatccg tgcttctggc tgcggcagac gaagctcatc gcgcatcggc
    28021 cggttgagcc atgggccggt gtcggaaatc cttggccacg tgccttcatt cagcccaccg
    28081 atgacgacga cgtcggggtg ttgcaggcgc gcttccagca gaccccagat cgataaccgc
    28141 ggatgcgccg gcaaagcgct gcgaacgttc tcgtcggcga cgaggttgcg atagaaatcc
    28201 ggatagtccg cagcggcgag gcgcggcgcg acgagagcgg gatcgatgag acccgcgaga
    28261 aggaccgcgg cggcctctcc cgcctcctcg ctccagaggg gcgaagccac agttcccacg
    28321 gcatctgctg gcgccgcgag cgcctccgcc accgccacat gcgcacgcac gaactcggcg
    28381 agggcctgct tgcccggccg gccgctcagc tcggtcaacg gctggaaggc ggttcgcagc
    28441 cgggcgagaa ggtcgcgcgc tgcccgccaa tctgaggtgc ccagctgccg ttgtgccttg
    28501 gttctcggct gttgagccgc gcgctcgatc gccgcctcga tgccgtcgag gccgcgtccg
    28561 aagtaatcgg tccggaaggc agcgagctcc agcgcgcgag cgacgcggcg tgcgtcgcgg
    28621 gcagagagcc caagcaatgt gaggggatgc ttgagcagcg ccatcacggc ggccggctcg
    28681 aaatcccggt cgaaggcgtc gatgacgaga tcgaggaagg tgccaggcac agtcttccgg
    28741 aacggccgcc cggccgagtc gtcgaccagg acgccccagc tttcgagccg gttcacgacc
    28801 ctgcgggcga ggatccggtc aggcgatacg agtgccgccg ttcgctcagg cgtctccagt
    28861 gcctcacgca ggatcagcgc gatcacctcc gcttcgtcct gcgcggtcgg cgcttcgatc
    28921 agggacaccc cgcgtaacgc atcgcggacg gcgccgggat cggtcgtctc gacgaaccgg
    28981 ctccacaggt gggtcgtgct ggccggccgc aaggcttcgc tcacgaaggc ggcgcgcaac
    29041 atggaaggtc cgtccgcctc agagccgggc aggcatttga cggcagaccg cgagacaccg
    29101 aggcgggaca gcaactttga aaggccgtgc tggggatgct ccggatgctc ccgcaagcgc
    29161 ccccagcccg cttcatcgag cgtctgatcg aggccgggca gcacgatggc gccgttctgc
    29221 aatcccagca cgacctgcat cagctcggcc gtcgccggta tgctgccggt gacgccagca
    29281 acgatgacgg gggcggtggg cggattggcg gccagccgtt ccgcctcggc aaggatcagc
    29341 cggttgcgcc ggtccatcgg ggaagtgagg ccgcgctcgg ccagatgctt cggccaccag
    29401 tcgagcacga tcttgaggaa ctcgagggtc tgctgccaat gctctgagaa cggctccggc
    29461 acgagatccg gcaggcgatc gaggctggcg ccctccgttt cgaccatgtc gatgaggcgc
    29521 ccgagttctc gagcgaggct cgcggcctgg gcaggtgtgc gcgcgcctgc gccagtgtaa
    29581 gggaggatat cgccgtgctc ggtcggatgc gcgctccgca tcgtctccga ccatttgaga
    29641 acgagctcgg tcagcacgag gcggcgctcg agctcgctga tggccggcgg aatgtcggta
    29701 gatgctgtgc cggaggcggg tggtgcggcg agttccgaga ggagcgtgag gtcctcctcg
    29761 actgtcgcga tcgggcggat tttcggcagc aggagcgctc gctttccgga tgcgacccgg
    29821 aaagcctcct gcagagcgcg tgcggcgcgc cgggtcggca gcaggatcgt cgttttcggc
    29881 agatccagcg gatcgggtgg attgcatcct tgccgcggca ggtcgcccga cagaattgcg
    29941 cgcgcgagtg ccggcaggaa aggttggccc ggtggaacgg tgaagaggcc ggcagcagct
    30001 tccgccttcc gggcaggtgc ggctggtctc gcgccaccct caggcggcat aacttcctcc
    30061 aagggcctgc tcggcttccg cgagcgcggc cggcgtgccg acatgcatcc aagtgccgtc
    30121 gaggcggatc ccgtagagcc ggcgctgctg gatcgcgcga tcccagagct ggttcagcga
    30181 aaaggcgccc tccggagcgc cgtcgaacag ccgcggatgg gcgatcgaca cgccggtgaa
    30241 gacgaacggc gccagctctc gctccggccg gcgctgcagc aggccatcag gagacatcag
    30301 gaagtctccg cgtccgtcgt aaccgatgct ggcggacgac agggcgagca ggagaaggca
    30361 gtccatccgc tcggcatcga aactctgcgc cagtctgacg agatcggact gtacgccgtc
    30421 gatccagacg ctgtcggagt tgtggatcag gaagggctcc tggccgagct gcggcagagc
    30481 cttcttcacg ccgcctccgg tatccagaag ctgctcccgc tcgtcggata tcaggatgcg
    30541 ggggcgctgg cgggtcttca gatgagagat gacctggtcg gcgaagtgat gcacgttgac
    30601 gaccgcctgg gaaattcctg cttcggcgat gcggtcgagg gcgtagtcga tcaggggctt
    30661 ctggcggagg cggaccagcg gcttcggaac gaggtccgtc agcggacgca tgcggctccc
    30721 caatcctgct gccagcacca tcgccgattt cggaatccac gtcatttcat gacactcggg
    30781 gttctgccgt tgtttcaatc gggcgggatg cggaacgcca cagggaagtt cgcatcgtac
    30841 cacgccttga gcgggcgaag tctggcatgg gccagatcgc gttcgagata gccccagatg
    30901 cgcgggatgt gccgcaggta gccattcttg ccgtcgcgca tcgatagccg ggtgaagatg
    30961 ccaaggacct tcgtattccg ctgtgcgccg agggctgcat aggcgaaggc gaaggttacg
    31021 cggtcgaacg ccggatcctg cgatgccatg gcggcgcagt agtgatcgaa caattcgtgt
    31081 tcgagagcct gcggaacatc gactcgcgca tcctgtagca gcgaagcgag atcataagcc
    31141 gggtgccctt cgaccgcgtc ctggcaatcg agaatgccaa cacgctcgag gccactccgg
    31201 tctggcagcc acacgaggtt cggcgaatgg taatcccgca gcaccaggcc ggtcgggagc
    31261 ggcagcagat cgtcgaagat gccgttccaa gcggccaggt attcggaccg cagcgcgtca
    31321 ggcgcggctt gcccgtagag cgccggccaa taccaatcca ggagaagctc gacctcgatc
    31381 tgcatggccg cccgatcata tctcgggagc tgatgactgg agccgtcgct caaacgaatg
    31441 tgacggggaa cgggcactga gcggaggtgc acgagaacgt cgacggctga gcgccacagc
    31501 tccgcctgat cgacgccttc ctggccggcg atctggctga acacgttatc accgaagtcc
    31561 tccacgagca gcaggcctgc atcgagatcc tgcgcgatca cccgcggcgc ggagagcccg
    31621 gcagacctca atgtgctcgt cacggcgacc cagggccgca aatcctccgc caaatgagcg
    31681 atgcggctgt aaggcttgcc atcacgaata ggcggtccat ccggctggcg gggcgcatcc
    31741 atcagaatgg cgctgtcgcc attgcggctg aggcgcgcga agcggcgggc ggacgcgtcg
    31801 ccttggaggt aacgcaggcg ggcatcgccc cacccggact gctgcaggaa ttcatcgatg
    31861 atccggagcc gttcgatccg cggctgaagg gtgccaagcc cttcgatggt tatgcggcgt
    31921 tccgatggat caaggctcgg atcttcctcc aggtgaacct cgatccggtc ggcgggaaga
    31981 agttcttcgg cgcgctctgg ccactcgatc agtgcagcgc ccgttgcgat gcactcctcc
    32041 aagcccaact cgcgggcttc atcggcgctg ccgagtcggt agagatcgaa atgggagacc
    32101 ggaaaacgcg gtgtggcgta ggtctggaca aggctgaagg tcgggctcgg cacctcggct
    32161 gcttcgtcct gcagcagcac ccggatgatg gcgcgagcaa gcgtcgactt gcctgcgccg
    32221 agatcgccac gcaacgcaac gacgtccccc ggcctgagcg cgtacgcgag cagctctgcc
    32281 agaggagcga tatcggtctc gcgcacgcgc ggatgtatcc agggtgccgt catgcggcgg
    32341 aaggaaaagc cgcacgcggg cggaacgtca agcctgagct gccatggcct ctgcgaccgt
    32401 cagctttgcc gtgacctccg ggaaccgcac agtgacccgc gtgccacggc cgggctcgga
    32461 ctcgagctgc atggtcccgc cgtggagctc gacgagtttc ttggcgatcg acagcccaag
    32521 cccgacgccg cgatgcttcg agccgaagct gcggctttcg aaacgctcaa agatgcgatg
    32581 ctgcaggtcc ttcgggatgc cgatcccctg gtcttccacc gagaaaacga ccatgccgtt
    32641 ctcccgccag caggtcacct tgacggtatc gcccggcttc gagaaaccaa cggcgttgga
    32701 cagcagattg tagatgatct gccggacgcg ggcctcgtcc gctacgaagt cgacggcgtc
    32761 gtcggcaacg gcgatgtcga gcgtcagacg ggcgcggctt gcccgctcgc ggacgccgag
    32821 aatggccgag tcgaggacgt gccgcacatc gactcgcgcg agcttcaact cgactgcacc
    32881 tgcctcgagg gtggcgaagt cgagtatgtc gtcgatgatc gagagcaggg ttttcgaaga
    32941 gctcgagatg tcgctgacgt actcgcgctg cttctggttg agctcgccgg cccgtgggct
    33001 cgacagcagc tcgctgaacc cgatgatgtt ggtcagcggc gtgcgcagct cgtacgagac
    33061 atggctgatg aactggctct tgaggcggtc ggcctcgacg agggcctcgt tgcgctcggc
    33121 cagcgcccgc tcgatgcgct tcgatgccgt cacgtcggcg aaggtgacca gggtggcgcc
    33181 gtcgggcaat ggcaccgcgc tgaagtctat gacgctgccg tccgggcgca ccatctggcc
    33241 ctcgacgctg tcgcgatggt ccgaaatggc cgtaacggcg cggctgatcc gcatccaggt
    33301 gcggcggttc tcgaaccgct cgcaggcgtg gcgaatgatc tcgtcgatat gcggctcccc
    33361 ctcgagcacc tggcgcggca gcctccagat gctggcgaat gcggagttcg aaagtttgag
    33421 ccgtccgttg gtggcgaaaa cggcgacgcc ctctttcagg ttgtcgagtg tttcgcgctg
    33481 gacgccgatg agcgcattgt agcggctttc gagagcgtag cgctcggtca cgtcgtcgaa
    33541 cagatgcgtc acgccgccgt cggggcgcgg ctcgacgacg acacgcaaca tcctcccgtc
    33601 cgggagatgc caccagtcct cgagcatggc cgccgtttga taacaggcca gcagcttcgc
    33661 tttccagtcg cgatagttga cgatcgccgg gaggcgggag gtctcgcgca ggcggtccag
    33721 gatctcgcta tcgctggggc tggattccag ccattcccgg tcgagatccc aaaggcgctg
    33781 gtaggcgacg ttgaagaagc tcagccgctg gtccggaccg aagatggcga ccgcggagga
    33841 gacgcgatcg agcgtccggt cataggcctc gatctgccgg tccagctcgc cctgggccgt
    33901 ttcgagggcc gccacgtcga tcgcggcgga agcgatggtg tcctcgagcg gcacggtgat
    33961 cacatcgtgc ggcttgacca caccgcccac gatgagcggc atgcgcttga tgacggtttc
    34021 ccgcttggca atgtggggct ccagggccgc ccgttgccgc gattccagga gttcgatctg
    34081 ctgttcgcgg acctccgcct cgtcggcggc gtcgacggcg gcgacgtagg ccttgttcac
    34141 ccacacgatt ttgccgctgg ggctcttgag ccagaccggc atgggcagcg cattcagcag
    34201 ggcgcggctc gagcgaatgt cacgggccag ctggcggtgc tggtcgatga tcttgatcag
    34261 gtcgcgcttg tagccggcca cgtcgcgcat ccgcaggaca gcgcgggcac cggccgcccg
    34321 tccgtctgcc tcgagatgtc cgccggccac tgtcttgagc agcaagttga acggccgccc
    34381 gtcggcgaag agcgcgtcaa gtccttgctt gagcgcctcg gcggagtcgg tatcgagcca
    34441 ctggccaaag cgcaggaact ccttctggtt ctccggcagg cccgctacgg aggtcagcgt
    34501 gtgggagacc accttcaatg gctggccctg ctcccagaac acgagcacct gcggctcggc
    34561 ctttacgatc gcctccgcgg tcgtcagatg acggcgcagc gatgcggctt ccgtctcggc
    34621 acgcctgatg gccagtcggg cccgccgcag catgtaccaa aggatagtga tccccagccc
    34681 tgcggccccg gtggcgagag cggcgaacag gagctggcgg atgctgaagc cagcgacacc
    34741 gctcgcccgt gtttcaacca gccccagcgt caaaatgaag atgcaggcaa ggctcgccag
    34801 gagaagcgca aggatctccc ggcgtgtggc atctgtccgg ctctgggggt ctgccacgtg
    34861 cattgcgttc catgcgaact ggcggcgcca attggaacgc gatacctggc gcagtggacg
    34921 ggggtgcctg acgggcgaat caccccaaat tatggggagg atcgcttgcg ctggccagag
    34981 tcaccggtgc cagcagagcc agcaattcgc agcacgcgcg ccaatggtaa ccgagcggca
    35041 tcactctcgt atgcaactgt cgctcgggtg atgaggtctg agccgctcag acgaaaaggc
    35101 tgcccgaggg caatggcacc atgagaagct tggcgaagag gacgtagaaa ccgatcacga
    35161 tggccagccc gacgagggga tagacgacga ttcgcgacgg agtccatggg tcgtgcatgg
    35221 cgatcagcag cagggctgcg aagctgatga tggccgccac caggaaaccg agccagggca
    35281 tcgccagcgc ggccgccaac at
//
LOCUS       DASVDU010000217         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1435881, whole genome shotgun sequence.
ACCESSION   DASVDU010000217 DASVDU010000000
VERSION     DASVDU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435881"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF226_13950"
     CDS             complement(<1..190)
                     /locus_tag="VF226_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075139.1"
                     /translation="MTLTRRSFATATVGFATTFCLPAWAQMAAPKRQFLSLATSSVGG
                     TWYPLGSAMAKVISKHYPQ"
     gene            318..983
                     /locus_tag="VF226_13955"
     CDS             318..983
                     /locus_tag="VF226_13955"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7075140.1"
                     /translation="MTGSPALQPIDTTPMHDRAYRALRRAIMAGVYAPGETISLGRLA
                     KALGTSIMPVREALRRLAAERAVEIIPKRGVRIPQITREKYEDLGRVRLELEGMAAEM
                     AAHRITGTELESLELYCAQMNEIAHDGARWQEYVVINCDFHFTVYRSSHSEVLLPLIE
                     ILWLQSGPLLSLYRERGIRRKPGLHEAIISALKDRDAVAAREAVQADLWNGIDFIRSV
                     APF"
     gene            complement(1009..1788)
                     /locus_tag="VF226_13960"
     CDS             complement(1009..1788)
                     /locus_tag="VF226_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7075141.1"
                     /translation="MSNPVLWLVDERGVARVALNRPEVNNAYNDAMIEGLHAAMDALA
                     GDAKVRAVVISGNGRHFQAGADLTWLNAVRAGSPEENEHASRRTAEAVDRLNRLPVPT
                     VALVQGFCVGGGTGLIAACDIVIAAEDAKFAISEVRWGLTAAIIIPQLADAIGARQIR
                     RYALTAERFTAEEARRIGLVHEIVPAADLEAAGERIIGALLANGPEAMAETKALALEH
                     ASGKVDADIMDALVVSHALKRQSDEAGEGLASFAEKRPARW"
     gene            complement(1846..>2857)
                     /locus_tag="VF226_13965"
     CDS             complement(1846..>2857)
                     /locus_tag="VF226_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7075142.1"
                     /translation="LKGFVALLIGLAVATIGIDPAAGHPRFTFGSVEMLQGISFIPAM
                     IGLFAVSELLRGVVTIGQAGDVLKQQVGNVFRGLGGVWRRYWRNFFRSSLIGTGIGAL
                     PGAGADIAAWIAYAVSKRFSREPEKFGTGHLEGIVDATSANNSAVAAAWIPALVFGIP
                     GDSITAIVIGVLYMKNMNPGPSVFLQNPQLIYAVFIIFVLANLMMVPIGWTAIKLAKQ
                     TLRTPRNILLPIILLFCIVGSYAMTNSPYGIIIMLVLGILGWILEENGFPVGPMILGM
                     VLGELFEQSFMTSMIKADGSLLGFFSRPIAGTLGVLTLALWAYMLLRNVRLGRQPQPE
                     KQ"
BASE COUNT          571 a          882 c          887 g          517 t
ORIGIN      
        1 gctgcgggta gtgcttcgag atcaccttgg ccatcgcgct gccaagcggg taccaagtgc
       61 caccgacgct cgaagtggcc agtgacagga actggcgttt cggggccgcc atttgggccc
      121 aggcgggcag gcagaacgta gtggcaaagc ccaccgttgc cgtcgcaaat gacctgcgtg
      181 tcagggtcat gcttttctcc tcccgaggct aattcgaagc gccttgcgct ggcgctttcg
      241 cggtcattat ttccgctcaa cgatcccatc ttgtggtaac tatgatcgaa gatcaaggat
      301 aaatccaagt cgctctgatg accggatccc cagccctgca gccgatcgat acaaccccga
      361 tgcacgaccg ggcttaccgg gccctgcgtc gcgccatcat ggctggcgtc tacgcgccgg
      421 gcgaaaccat cagcctcggc cggctcgcga aggcgcttgg gacaagcatc atgcctgtgc
      481 gggaagcgct gcggcgtctc gctgctgagc gcgccgtcga gatcataccc aagcggggcg
      541 tgcgcattcc tcagatcacg cgggagaaat acgaggacct tggccgggtg cggctcgaat
      601 tggaaggtat ggcggccgaa atggcggcgc accggatcac cggcaccgag ctcgagtcct
      661 tagaactcta ttgtgcgcag atgaacgaaa tcgcccacga tggcgcgaga tggcaggagt
      721 acgtcgttat caactgcgac tttcacttca ccgtctatcg gagcagccac tcagaggttc
      781 tcttgccact gatcgaaatc ctttggctac agagcggccc gctcttgagc ctctacaggg
      841 agcgaggcat ccggcgaaag cccgggctcc acgaagcgat tatttctgca ctcaaggata
      901 gggatgcagt agctgctcgt gaagcggtgc aggccgacct ctggaatggc atcgacttca
      961 tcaggtcggt ggcgccgttt tgagctttat tccgattgca agctggggct accaccgcgc
     1021 tggccgtttc tccgcgaacg aggcgaggcc ttcgccggct tcatcggact gacgcttcag
     1081 agcatggctc acgacgaggg cgtccatgat gtcggcgtcg accttgcccg acgcgtgctc
     1141 gagtgcgagg gccttggtct cggccatggc ttcggggcca ttggcgagca gagccccgat
     1201 gatccgctcg cctgctgcct cgagatcggc cgctgggacg atctcgtgta cgagcccgat
     1261 gcggcgtgct tcctctgcgg tgaagcgttc ggctgtcagc gcgtagcggc ggatctgacg
     1321 cgcgccgatg gcatccgcga gctgcggaat gatgatggcg gccgtcaaac cccagcgcac
     1381 ctcggagatg gcaaatttag cgtcttcggc cgcgatgacg atatcgcaag ctgcgatcag
     1441 gccggtgccg ccgccgacgc agaagccctg gaccaatgcg acggtgggca ccggcagcct
     1501 gttcagcctg tccaccgctt cggccgtgcg gcgcgaggcg tgctcgttct cctcgggcga
     1561 gccagcccgg acagcattga gccaggtcag atcggcccca gcctggaaat gacggccgtt
     1621 gccgctgatg accaccgcac gaaccttagc atcacctgca agtgcgtcca tcgccgcgtg
     1681 caggccctcg atcatggcat cgttgtaggc gttattgacc tcgggacggt tgagcgcgac
     1741 gcgagcgacg ccacgctcgt cgacgagcca gaggacggga ttggacatct tccactccat
     1801 ccctgagtgt tcagggccag accccggcag gtctggccct ttctttcatt gcttctcggg
     1861 ctggggctga cgccctagcc tgacgtttcg cagcagcatg taggcccaga gcgcgagcgt
     1921 gaggacaccc agggttcccg cgatcgggcg cgagaagaac ccgagcagcg agccatccgc
     1981 cttgatcatc gacgtcatga agctctgctc gaagagctca cccagcacca tgccaaggat
     2041 catcgggccg accgggaagc cgttctcctc gagaatccag cccaagatac caagcaccag
     2101 catgatgatg atgccgtagg gggagttcgt catcgcataa gagccgacga tgcagaacag
     2161 caggatgatg ggcagcaaaa tgttgcgcgg ggtgcgcaga gtctgcttcg cgagcttgat
     2221 ggccgtccaa ccgatgggca ccatcattag attggcgagc acaaagatga tgaacacggc
     2281 atagatgagc tgcggattct gcaggaacac ggacgggccc gggttcatgt tcttcatgta
     2341 gagaacgccg atcacgatgg cagtgatcga gtcacccgga atgccgaaca cgagcgccgg
     2401 aatccaggct gcagccaccg ctgaattgtt ggccgaggtt gcatcgacga tgccctcgag
     2461 atgccccgtg ccaaacttct ccggctctct ggaaaagcgc ttggagactg cgtaggcgat
     2521 ccaagcggcg atgtccgcgc ccgctccggg cagggcacct atccctgttc cgatgagaga
     2581 gctgcgaaaa aagttgcgcc agtaacggcg ccagaccccg ccaagtccgc ggaagacatt
     2641 gccgacctgc tgcttcagca catctcccgc ctgaccgatc gtgacgacac cgcgcaacag
     2701 ctccgagacg gcgaataggc cgatcatagc cgggatgaaa ctgataccct gcagcatctc
     2761 gacggagccg aacgtaaaac gagggtgccc ggccgctgga tcgatcccga tggtcgcgac
     2821 ggccaatccg atcagcagtg caacaaatcc cttaagg
//
LOCUS       DASVDU010000218        17274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1167101, whole genome shotgun sequence.
ACCESSION   DASVDU010000218 DASVDU010000000
VERSION     DASVDU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17274
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167101"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="VF226_13970"
     CDS             <1..1053
                     /locus_tag="VF226_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX7075143.1"
                     /translation="WKARGLDFSKLFHKPEVDSSVAIYNSERQDHGLDKVLDRKLIEL
                     AKPALEARKPVRHELKITNRDRAVGAMLSGEIAKRYGHEGLREDTIWLTLQGTAGQSF
                     GAWLARGVTLDLVGEANDYVGKGLSGGKLIVRPHPNAGVKPEESIIVGNTVLYGAISG
                     ECYFRGIAGERFAVRNSGAVAVVEGTGDHGCEYMTGGVVVVLGPTGRNFAAGMSGGIA
                     YVLDERGDFEQRCNLAMIDLAPVEAEEEVMRRLANQGGDLESHGLVDILSDMDRKDAE
                     RLHQLIERHARYTNSAKARMILADWRSWLPKFKKVMPVEYREALRQMAEHQANDKTGL
                     EVLEIGLPEAHKPPAE"
     gene            complement(1059..1469)
                     /locus_tag="VF226_13975"
     CDS             complement(1059..1469)
                     /locus_tag="VF226_13975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7075144.1"
                     /translation="MKTVTNAFFDTNVVAYFASEDATKADIAARLLRTGGVVSVQVLN
                     EFASLAVRKRRWPISQVRQVLEAVRETCRVVPLTPDTHDLGLTVAERYRLNIYDAFVV
                     AAALMSDCTVLYSEDLHDGLIIDGLAIRNPFVAR"
     gene            complement(1453..1710)
                     /locus_tag="VF226_13980"
     CDS             complement(1453..1710)
                     /locus_tag="VF226_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7075145.1"
                     /translation="MRVSKWGNSLAIRLPAKLAAALGLKEGDRIDVRPAGHEEPPIVE
                     IERKLTIDELFERVRNMPNKLPANYKFDRRDAVRPGYEDRD"
     gene            1807..3243
                     /locus_tag="VF226_13985"
     CDS             1807..3243
                     /locus_tag="VF226_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX7075146.1"
                     /translation="MGKITGFLEFERIDRSYEPVEKRIRHWREFVVPLPENEVRTQAA
                     RCMDCGIPYCHNGCPVNNQIPDWNDLVYKGDWQRAALNLHSTNNFPEITGRVCPAPCE
                     AACTLNIDDKPVTIKTIEYSIAERAFQEGWIVPQPAERKTGKRVAIVGSGPAGLAAAQ
                     QLARAGHDVHVYEKNAKPGGLLRYGIPDFKLEKHVIDRRLKQMEAEGVVFHCGVHVGA
                     DVSARQLEAEYDAVLLTGGSERPFDFFANAPGRELDGIHFAMEFLPQQNRRVSGESVG
                     EVKEITAKDKHVIVIGGGDTGSDCIGTSIRQGAKSVTQLEIMPMPPEKENKALSWPYW
                     PVKLRISTSQAEGAKTEYSISTTGFSGRNGKVEKLLYTRVDAKLQPIPGSEGELAADL
                     VLLAMGFSGPVESGLVKELGLEPVPRGRFKGLDADERDYRIKGSKKLFAAGDMRRGQS
                     LVVWAIREGRQAAHAIDKALMGRSDLPR"
     gene            complement(3311..4729)
                     /locus_tag="VF226_13990"
     CDS             complement(3311..4729)
                     /locus_tag="VF226_13990"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX7075147.1"
                     /translation="MLDGSARRRVTSLVAGLVAGLAIGFSLLAAALLPSTARAQNEWG
                     ASYITPFPEDDVYRLKVFGDAMAEGVFGALTEAFRSDSRVRLPNSYDRVNGFIRMRPE
                     DFARFEESIKKEPLHIAVIMLGTSDRVALRAPDGRRVNVGSDEWKEEYGRRVDRITRA
                     LKRRGAAVYWVGLPIVSRSDMNEDVRMLNSVFRERALLNGIKFIDIYEAFADELGQYT
                     QYGPDLTGKQRLLRSGDGIYFTTVGYRKVAYFVEREIKRDLIQAKNERDIPLAGSEAE
                     QRRISPKAQADEWAGTVQRAPQEGENSAAAKASASAPQNSDSGSGALKAENSKVAIKI
                     AGASGKEEAVTIEILRPAIPESIVALVSRQHSQGHSAQIGDNIPETLPGGVTVLSSIT
                     PGLAAGSQAQRRRLAPTQSPFFKVLVKGERIPPKPGRADDFTWPPPDSAPPPRIERRS
                     QSSPIPLPKRNPQAAEAQPSHG"
     gene            complement(4737..5999)
                     /locus_tag="VF226_13995"
     CDS             complement(4737..5999)
                     /locus_tag="VF226_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX7075148.1"
                     /translation="MREARVSVTVSKVLSGLVLMMALMAGSATGETSEAQIAPAVDLR
                     PFLEQVRPEALRAGVSAATFDRATRGLEADPEVLMRAASQPEFVKPVWDYLSGLVSDA
                     RVAAGQQRLAEYGDTLAAIERAYGVDRHVVLAIWGVESNFGTAQGERNVIRSLATLAA
                     GDERRSAFWRREFIAALRIVERGDIAPERMTGSWAGAMGHTQFMPTTYHDRAADFDGD
                     GRRDIWDSVPDGLASTANYLKAFGWRLEDPWGFEVVLPEGFDYSLSAPGQAKPASFWR
                     RIGVSRPGGVAVRDTVSPLQLILPAGAQGPAFLVTGNFKAILRYNPAEAYALAVGHLA
                     DRIAGAEPIQGPWPESDRPLARAEREELQKRLAERGLDTGAPDGIIGTQSRAAIRAFQ
                     RTVGLPEDGYASLRLLDLLRQTGGSTRE"
     gene            complement(5996..7282)
                     /locus_tag="VF226_14000"
     CDS             complement(5996..7282)
                     /locus_tag="VF226_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX7075149.1"
                     /translation="MASAAVRPGTAGRLSEWVDRNVLLALAALFVALGVPVSAAGADE
                     PFKAWLADLWPEARAFGISRQTFDAAFRNVEPDLRLPDLAIPGRQQAPRGQAEFTRPP
                     QAYINSKQLARLAAAGRALRAEHAGTLEEIERQFGVEGEAVLAIWGRETAFGAHQLPH
                     YAIRVLATQAYLGRRKEMFRNELLHALKLIDDGSASIEQMRSSWAGAIGLPQVMPSEF
                     HRWSYDLDGDGRKDIWTSIPDALATIARQLQGKGWVYGQTWGYEVRLPQSVDCSLEGP
                     ENARPLSAWAAMGVERTFGRQFPKHVLQAEAYLMAPGGAYGPAFLVLENYRVIRRYNM
                     SDLYAVFVGNLADRIAGGGDFETPWQNIAQLPAKDVEEIQQRLKELGYAIGKVDGKVG
                     SFTRWQIGKFQHDNRLKVDCWPTRELLRHLRSAVAP"
     gene            7688..8566
                     /gene="galU"
                     /locus_tag="VF226_14005"
     CDS             7688..8566
                     /gene="galU"
                     /locus_tag="VF226_14005"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX7075150.1"
                     /translation="MTSLRKPIRKAVFPVAGLGTRFLPATKAVPKEMLPVVDRPVIQH
                     VVDEARAAGIEHLVFVTGRNKAVIEDHFDSAYELERTLAERGKTKDLEQLQRELPAAG
                     ATSFTRQQAPLGLGHAVWCAREIVGDEPFALLLPDMLHHGDPPCLAEMMAAYAEHGGN
                     HVAVAAVPEDQTHLYGIVGVEDSTAKVSRITSMVEKPPPGTAPSNLHISGRYILQPEI
                     FGLLEKQERGAGGEIQLTDSMLKLAEQQPFYAVRFDGKIYDCGSKIGFLAANVAYALE
                     RDDLGPALREELKQLL"
     gene            8954..9658
                     /locus_tag="VF226_14010"
     CDS             8954..9658
                     /locus_tag="VF226_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7075151.1"
                     /translation="MRFRLMLGAACACFAALPALAADLGGPRRLPPPANFDEPFIPDS
                     KPPLWQGLYWGLSGGYGWGSSDASFDPGVNSASTDPEGFLASATVGYNMLLGSSLLVG
                     VEGDLGVMDISADDEDVGGALYRTSFGPFWGTLRGRAGIPIGPLLLYGTGGLAFASLD
                     EVSIETGDEKASDSELRAGWVAGGGAEFAFSESTTAKVEYLHMEFDSTDGLTSDGSFS
                     FDNKLDVVRAGLNFRF"
     gene            complement(9706..10539)
                     /gene="moeB"
                     /locus_tag="VF226_14015"
     CDS             complement(9706..10539)
                     /gene="moeB"
                     /locus_tag="VF226_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX7075152.1"
                     /translation="MTKLTPQEIERYKRHLVLREVGGQGQQKLKDARVLVVGAGGLGS
                     PVIMYLAAAGVGTIGVIDDDRVSLDNLQRQIVHETAQVGTPKVESAKLAVERLNPHVT
                     VETHNCRLTAENAIDIISRYDIVADGSDNFATRYLTSDACYLAKRTLVFAAVGPFDGY
                     VTVLKPHLKNAEGEPWPSYRCIFPEAPPPGTVAPCSEIGVLGSVVGVIGTLQATEVLK
                     EILEMGDSLAGRLLIYDALSARFEIVKVAWDPDNPLSGRNPTIRDLSIHSEPIGEVCV
                     A"
     gene            complement(10639..11199)
                     /gene="dut"
                     /locus_tag="VF226_14020"
     CDS             complement(10639..11199)
                     /gene="dut"
                     /locus_tag="VF226_14020"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7075153.1"
                     /translation="MPRAPDDTIEVRIVRLPHAEGLPLPAYQSDEAAGMDLLAAVEED
                     APIVLAPGERQLIPTGLVMELPRGLEGQVRPRSGLALRHGITVLNSPGTIDSDYRGEV
                     KVLLINLGGAPWEIQRGERIAQLVFQRVARADLVEVATLNDTRRGAGGFGSTGTAGVA
                     LAPQPRIPIKSAASTRAKKSPEKPRE"
     gene            complement(11199..12476)
                     /gene="coaBC"
                     /locus_tag="VF226_14025"
     CDS             complement(11199..12476)
                     /gene="coaBC"
                     /locus_tag="VF226_14025"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7075154.1"
                     /translation="MCLVSRLCKPAVIDVTDKRILLIIGGGIAAYKCLDLIRRLRERG
                     MRVRTVMTAAAQQFVTPLAVGAISGDRVFSDLFDREDEHDVGHIRLSREADLIVLAPA
                     TASLLAKMAGGHADDLATAVLLATDKPVLAAPAMNPRMWLHPATQRNLAMLQADGIHF
                     VGPNTGEMAEQGEAGPGRMAEPLEIVAAVEALLRRPAAKGPLSGRHVLVTSGPTHEPI
                     DPVRYIANRSSGKQGHAIARAAAELGARVTLVSGPVAIADPPGVDVVHVETAREMLAA
                     VEAALPADIAIFAAAVADWRVANAASQKIKKDKSAAAPALEFVENPDVLKTIASRSPR
                     GERRPALVIGFAAETENVVEQAKKKRKAKGCDWIVANDVSAEGGAMGGDRNTVHLITA
                     RTVESWPAMDKAEVARRLVERAAEHLAQTQVSV"
     gene            12803..13360
                     /locus_tag="VF226_14030"
     CDS             12803..13360
                     /locus_tag="VF226_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7075155.1"
                     /translation="MELVTHFRRMARNNAWSNWRLLDACSRLSPEEFRAPRTSFFPSI
                     QATLDHILCVDLYYLDALESGKLGYAYFESRRPIEEFAVLRTDQIATDQRLIGFCDRL
                     TPDDLRRIIGIDRGPKGIDYETVGDTLSHLFVHQIHHRGQVHAMLAGTNVKPPQLDEY
                     FLQYDRDIRAPDLAALELEGPESLV"
     gene            complement(13579..14367)
                     /locus_tag="VF226_14035"
     CDS             complement(13579..14367)
                     /locus_tag="VF226_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX7075156.1"
                     /translation="MLDFRTTDPEGGAITLGTGRKPAAPPQECKTVLVILHQLHSNPG
                     HVGHWLRMRGYTLDIRRPRFGDPLPETLEHHAGAIIFGGPMSANDPDDYIRLETEWIG
                     VALKERKPFLGICLGAQMLARQLGARVYEHPERRVEIGYHPIRPTPHARSFGDWPRCV
                     YQWHREGFDRPHGATVLATADGPFENQAVVYGETAVGVQFHPEITYAMVKRWSGSHPE
                     RLTLPGAQDRPSQLSNHLAYGPNVRRWLDTFMPRWLESHARQAA"
     gene            complement(14354..15235)
                     /locus_tag="VF226_14040"
     CDS             complement(14354..15235)
                     /locus_tag="VF226_14040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075157.1"
                     /translation="MITRLADLWVRRRWRPNPEAMAQWADLRPAVVVTGGSEGIGFEI
                     ARRFARRGSTVVLVARRPDRLEEAAAAIRGEFGTAVLTISLDVTRPDAAEVIETELRG
                     RGFYVDVLVNNAGMGLSGDYIEQPGDRIAALVELNIRAVSLLMRHFLPDMCVRGRGGV
                     LNVGSLGGYAPGPYQAAYYASKAYVISLTEAVAWESGGRGARIAVFAPGPIRTRFHRR
                     MGAEHALYRWLIPAPGPSTAAGTAVRWFEWGRTVITPGVLGPLLAIAMRLIPHPVLLP
                     IVGWLLKPRELLKNAGL"
     gene            complement(15282..16253)
                     /locus_tag="VF226_14045"
     CDS             complement(15282..16253)
                     /locus_tag="VF226_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7075158.1"
                     /translation="MTRDIDIALLRAFLAVVETGGVTTAARILNRTQAAVSLQIKRLE
                     ELFGTELFLRERKRFTLSPAGERLFGSAQRLVALNDATWGAMTTPQFEGEVRFGVPPD
                     IVTSLIPPVLQRFHQAWPRINVSLICKASASLIEDLAGRKVDLILTTEIDCGPAGETL
                     LSDRLVWVGGPGSNTHLQRPLPVSLGTKLCRFRPEVIKALDAAQIDWRPVSQDCNMEP
                     LYATVKAGLAVAPLIRTSVPDYLKILGAGSGLPPLPDFRVNLYLPAGATEITRELARY
                     IRQTIELRFGPSRGQMQRRPEVRQRVTATTGRRRAPRAGVLRPVAAE"
     gene            16411..16641
                     /locus_tag="VF226_14050"
     CDS             16411..16641
                     /locus_tag="VF226_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX7075159.1"
                     /translation="MTSTTQSAWQASHGQAIPRTGRFLRIFDRLWLAYKVYRERQALL
                     ALSDYELRDIGLSRADARREANRPLWDLPEIR"
     gene            17060..17136
                     /locus_tag="VF226_14055"
     tRNA            17060..17136
                     /locus_tag="VF226_14055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17094..17096,aa:Arg,seq:acg)
BASE COUNT         3101 a         5683 c         5504 g         2986 t
ORIGIN      
        1 tggaaggcgc gcggcctcga tttctcgaag ctgttccaca agccggaggt cgacagcagc
       61 gtcgcaatct ataacagcga gcgccaggat cacggcctcg acaaggtgct cgaccggaaa
      121 ctcatcgagc tggcgaagcc cgctctcgag gcgcgcaagc cggtccggca cgagctcaag
      181 atcaccaacc gcgaccgcgc cgtcggcgcc atgctctcag gcgagatcgc caagcgctac
      241 ggccacgagg gcctgcgcga ggatacgatc tggctgacct tgcagggaac ggccggccag
      301 agcttcggtg cgtggctcgc acgtggtgtc accctcgacc tcgtcggcga ggcgaacgac
      361 tacgtcggca agggcctctc gggcggcaag ctgatcgtcc ggccgcatcc gaacgcaggc
      421 gtgaagcccg aggagtcgat catcgtcggc aacacggtgc tttacggcgc catcagcggc
      481 gagtgctact tccgcggcat tgcgggcgag cggttcgccg tacgaaactc tggtgccgtg
      541 gcggtcgtcg aaggcacggg cgatcacggc tgcgagtaca tgacgggcgg cgtcgtcgtg
      601 gtgctggggc cgacggggcg caacttcgcg gccggcatgt cgggcggcat tgcctacgtg
      661 ctcgacgagc gcggagattt cgagcagcgc tgcaacctcg ccatgatcga ccttgccccg
      721 gtcgaggctg aggaggaagt gatgcgccgc ctcgccaatc agggcggcga tctcgaaagc
      781 catgggctgg tggacatcct ctccgacatg gaccggaagg acgccgagcg cctgcaccag
      841 cttatcgagc gccacgcccg ctacacgaac tcggccaagg cccgcatgat cctcgccgat
      901 tggcggagct ggctgccgaa gttcaagaag gtgatgccgg tggagtaccg cgaggcgctg
      961 cgccagatgg ccgagcacca ggccaacgac aagacggggc tcgaagtgct cgagatcggc
     1021 ctgcccgaag cgcacaagcc tccggcggag tgatcagctc agcgcgcaac gaaaggattg
     1081 cggatcgcga ggccgtcgat aatcaggccg tcgtgcaggt cttcggaata caggacggtg
     1141 cagtcggaca ttagggccgc cgcgacaacg aaagcatcat agatgttgag cctatatcgt
     1201 tcggcaaccg tcagaccgag gtcatgtgtg tcaggcgtta gtggcacgac ccggcacgtt
     1261 tctcgcacgg cctcgagcac ctgccggact tggctgatcg gccagcgccg cttgcggacg
     1321 gccaagctgg caaactcgtt gagaacttgg acgcttacga cgccaccagt ccgaagcagt
     1381 cgggccgcta tatcggcctt cgtagcatcc tcggatgcga aatacgcgac gacgttcgtg
     1441 tcgaagaacg cgttagtcac ggtcttcata cccgggccgc acagcatccc tgcgatcgaa
     1501 cttgtagtta gcaggcagct tgttgggcat gttcctcaca cgctcgaaca gctcgtcgat
     1561 agtcagcttt cgttcgatct cgacaatagg gggttcctcg tgcccggcgg gacgcacgtc
     1621 gatcctgtcg ccctccttca agccaagggc ggcagcaagt ttcgcgggca gacgaatagc
     1681 caggctattt ccccacttgg aaacccgcac ctccatcgct cagctccaga tatacattaa
     1741 tcaagatgta tatctaggaa catctcaatt gggtagcaag cgacgccatt acgaaggcaa
     1801 gcagcaatgg gcaagattac cggcttcttg gaattcgagc gcatcgaccg gagctacgag
     1861 ccggtcgaga agcgcataag gcactggcgc gagttcgtgg tgccgttgcc ggagaacgag
     1921 gtgcgcacgc aggcggcgcg ctgcatggac tgcggaatcc cctactgcca caacggctgc
     1981 ccggtgaaca accagatccc cgactggaac gatctcgtct ataagggcga ctggcagaga
     2041 gccgcactca acctgcactc gacgaataac ttcccggaga taacgggccg cgtctgcccc
     2101 gcgccctgcg aggcggcctg cacgctcaac atcgacgaca agccggtcac cataaagacg
     2161 atcgaatatt cgatagcgga acgggccttc caggaaggct ggatcgtgcc gcagccggcc
     2221 gagcgcaaga ccggcaagcg ggtagccatc gtcgggtctg ggccggcggg cctggccgcc
     2281 gcgcagcagc tcgcccgcgc gggacacgac gtgcacgtct acgagaagaa cgcaaagccc
     2341 ggcggcctgc ttcgctatgg cattcccgac ttcaagctcg agaagcacgt catcgaccgg
     2401 cgtctgaagc agatggaggc cgaaggcgtc gttttccact gcggtgtgca cgtcggcgcc
     2461 gatgtctctg ccaggcagct cgaggccgaa tacgatgccg tcctgctgac cggcggctcc
     2521 gagcggccgt tcgacttctt cgccaatgca cccggccgcg agctcgacgg tatccatttc
     2581 gccatggagt tcctgccaca gcagaaccgg cgcgtttcgg gcgagagcgt gggcgaggtg
     2641 aaggagatca cggccaagga caagcacgtc atcgtcatcg ggggcggcga cacgggttcc
     2701 gactgcatcg gcacgtcgat ccggcagggg gcgaaatcgg tcacccagct cgagatcatg
     2761 ccgatgccgc cggagaagga gaacaaggcg ctctcctggc cctactggcc ggtgaagctg
     2821 cggatctcga ccagtcaggc cgaaggcgca aagaccgaat actccatttc gaccaccggc
     2881 ttttcgggcc gcaacggaaa ggtcgagaag ctgctctaca cgcgtgtcga tgccaaattg
     2941 cagccgattc ccggcagcga gggggagctg gcggccgacc tggtgctgct ggccatgggc
     3001 ttttccggcc cggtcgagag cgggctggtc aaggagctcg gcctcgaacc cgtgccgcgc
     3061 ggccgtttca agggccttga cgcggacgaa cgcgactacc ggatcaaggg cagcaagaag
     3121 ctgttcgcgg ccggagacat gcgtcgcggc cagtcgcttg tcgtctgggc catccgcgaa
     3181 ggccgacaag ctgcgcacgc tatcgacaag gccctgatgg gccgctccga tctgccgcgt
     3241 tgatgcggga catgacttgc tcggccatga gttttaaggg agcgacccgg agttcgaccg
     3301 ctcaccagct tcacccgtga ctgggttgtg cctcggcggc ctgcgggttc cttttgggaa
     3361 gcggaatggg gctggactgt gatcggcgct cgatgcgtgg tggcggcgca gaatccggcg
     3421 gcggccaggt gaaatcgtcg gcgcggccgg gtttgggggg gatccgctca cccttcacca
     3481 gcaccttgaa gaacggcgac tgggtcggcg caagacggcg gcgttgagct tgcgagccgg
     3541 cggccagccc cggtgtgatc gagctcagaa ccgtcacgcc gccaggcagc gtttcaggaa
     3601 tgttgtcacc gatttgtgcc gaatggccct gggagtgctg gcgcgagacc agcgcgacga
     3661 tggactccgg gatggcgggg cgcaggatct cgatggtgac agcctcttcc ttgccggagg
     3721 cgcccgcaat tttgatcgcg accttgctgt tttccgcctt gagagcgcca gatcccgagt
     3781 cgctattctg gggcgcgctg gcactcgcct tggcggcggc cgagttctcc ccttcctgtg
     3841 gcgcccgctg cacggtcccg gcccactcgt ctgcctgcgc ctttggcgag atgcggcgtt
     3901 gctcggcctc gctgccagcg agggggatgt ctcgctcgtt cttcgcctgg atcagatcgc
     3961 gcttgatctc gcgctcgacg aagtaggcga ccttccgata accaacggtc gtgaagtaga
     4021 tgccatcgcc gctgcgcagg agacgctgct tgcccgtgag gtcgggaccg tactgcgtgt
     4081 actggccgag ctcgtcggca aaggcctcgt agatgtcgat gaacttgatg ccgttgagca
     4141 gcgcccgctc acggaaaacc gagttcagca tccgcacgtc ttcgttcatg tcggaccggc
     4201 tgacgatcgg cagcccgacc cagtagaccg cggcgccgcg gcgtttcagg gcgcgcgtta
     4261 tgcgatccac ccgccgaccg tactcctcct tccactcgtc ggatccaaca ttgacccgcc
     4321 ggccgtcagg agcgcgcagg gcgacacgat cggacgtgcc cagcatgatg accgcgatat
     4381 ggagcggctc cttcttgatc gactcctcga accgagcgaa gtcctcggga cgcatgcgga
     4441 tgaagccatt gacccggtcg tagctgttcg gcaggcgcac gcggctgtcc gaccggaacg
     4501 cctcagtcag cgcaccgaac acaccttcgg ccatcgcatc gccgaagact ttgagccggt
     4561 aaacgtcatc ctcggggaaa ggcgtgatgt agcttgcgcc ccactcgttc tgggcacgag
     4621 ccgttgaggg caggagcgcg gccgccagca ggctgaagcc aatggcgagc cctgccacca
     4681 agcctgcgac cagggaagtc acgcgccgcc tggccgagcc gtcgagcact ggttcctcac
     4741 tccctcgttg acccgccggt ctggcgtagc aggtcgagaa gccgcaagct tgcatagccg
     4801 tcctcgggca agccgacggt gcgctggaaa gcgcggatcg cggcgcggga ctgagtcccg
     4861 atgatcccgt ccggcgcccc ggtgtcaagg ccacgctcgg ccaatcgttt ctgaagctcc
     4921 tcacgctcgg cgcgggcaag gggtctgtcc gactcgggcc acggaccttg tatcggctct
     4981 gcgcccgcta tccggtctgc cagatgaccg accgcaagcg cataggcctc cgccgggtta
     5041 taccgcagaa tggctttgaa gttcccggtg accaggaatg caggtccctg tgcaccggcc
     5101 ggcaggatga gctggagcgg tgagaccgtg tcgcggacgg ccacgccgcc gggacgcgag
     5161 accccgatcc gccgccagaa cgatgcgggc ttcgcctgcc ccggtgccga caggctgtag
     5221 tcgaatcctt ccggcaggac aacctcgaag ccccaggggt cctcgagccg ccagccaaag
     5281 gccttcagat agttggccgt cgaggcaagg ccgtccggca cgctgtccca gatgtcgcgg
     5341 cggccgtccc cgtcgaagtc ggcggctcgg tcatggtagg tcgtcggcat gaactgcgtg
     5401 tgccccatgg cgccggccca ggagcccgtc atgcgctcgg gcgcaatgtc tccccgctcc
     5461 acgatgcgca aggcggcgat gaactcacgg cgccagaacg ccgaccggcg ctcgtcgccc
     5521 gcggccagcg tggcgaggga acggatcaca ttgcgctcgc cttgggcggt gccgaagttc
     5581 gattccaccc cccagatcgc gagcacgacg tggcgatcca cgccataggc ccgctcgatg
     5641 gccgccagcg tgtcgccata ctcagcaagc cgctgctgcc cggcggcgac ccgtgcgtca
     5701 ctgacgagac ccgaaagata gtcccaaacc ggcttgacga actcgggctg ggaagcagcc
     5761 cgcatcagca cctctgggtc ggcttcgagc ccacgcgtcg cccggtcgaa ggtggccgcg
     5821 ctcacccctg ctctcaaggc ttcgggcctg acctgctcaa ggaaagggcg caagtcgacg
     5881 gctggagcga tttgagcttc agacgtttca ccggttgcag agccagccat gagcgccatc
     5941 atcaacacca gtcctgaaag gacttttgac accgttaccg acactctcgc ctccctcatg
     6001 gggctacggc agaccgcagg tgccgcagca gctccctcgt cggccagcaa tcgactttca
     6061 ggcggttgtc gtgttggaat ttgccgattt gccagcgcgt gaaggatccc accttgccgt
     6121 cgaccttgcc gattgcgtag ccaagctcct tcaacctctg ctggatttct tccacatcct
     6181 ttgcgggaag ttgcgcgata ttctgccacg gcgtttcgaa gtcgccgccg cccgcgatcc
     6241 gatccgccag attgccgacg aagacggcat agaggtcgga catgttgtag cggcgaatga
     6301 cgcgatagtt ctcgagcacc aggaaggccg gcccgtaggc gccaccggga gccatcaggt
     6361 aagcttccgc ctgaagcacg tgcttgggaa attgccggcc gaacgtgcgc tcgaccccca
     6421 tggcagccca ggcggagagc gggcgcgcgt tctccggccc ttccagtgag caatcgaccg
     6481 attggggcag ccgcacctcg tagccccacg tctggccata tacccagccc ttgccctgga
     6541 gctggcgggc gatggtggcg agcgcatcgg gtatggaggt ccagatgtcc ttccggccat
     6601 ccccgtcgag atcgtaggac caccgatgga actcggatgg catcacctgc ggcaggccga
     6661 tcgcaccggc ccacgacgag cgcatctgct cgatcgaggc agagccgtcg tcgatcagct
     6721 tcagcgcatg caggagctcg ttgcggaaca tctccttgcg gcggccgaga taggcctgag
     6781 ttgccagcac gcggatggcg taatgcggca gctgatgcgc gccgaaagcg gtctcgcggc
     6841 cccagatcgc cagcacggcc tcgccctcga ccccgaattg gcgctcgatt tcctccaacg
     6901 ttccggcgtg ctcggcgcgg agtgcgcgtc cggcggcggc aaggcgggca agttgcttac
     6961 tgttgatgta ggcctgcggc ggcctggtga actcggcctg cccgcgcggc gcctgctgcc
     7021 gcccggggat ggcgagatcg ggcaatctca ggtcgggctc gacgttccgg aaagctgcat
     7081 cgaaggtctg gcgagagata ccgaaagccc gcgcctcggg ccataggtcg gccagccaag
     7141 ctttgaatgg ttcatcggcc cccgccgcag acaccggcac cccaagagcc acgaacagcg
     7201 ctgccaacgc cagaaggacg ttccgatcga cccattcgct caatcgtcct gctgtgcccg
     7261 gccggacggc agcgcttgcc atcggcccac ctccctcgat gccgacccgc acactacgcg
     7321 gagcgcgatg accccacggc caaatcactt gccacgcaaa atctcacgtt ttctcggcga
     7381 taatggggct ggcgctcgca ggcgcgcctg ccgcgtaaac ggcttgccaa cacagcccgg
     7441 gacttcacag ccctgtcgtg gtaagacgca acccggggtt ggggctcgct ttggcaaaag
     7501 tctcgagcca gccccccctc tgacacgtcg agtcattctc tcccgtccag gccctgatct
     7561 tgccacatca tccgtgagtt aaccccgagg cgccaatcgg cgggcaaggc gttcgaaggt
     7621 ctcgtcggtc agcatgccgc gccaagtaaa aaattcggta agccggccag cagctttgga
     7681 gaactgcatg acaagcctca gaaaacccat tcgaaaggcg gtgttcccgg tcgccgggct
     7741 gggcacgcga tttttgccag cgaccaaggc ggtaccgaaa gagatgcttc ccgttgtcga
     7801 tcgtccagtg atccagcacg tcgtcgatga ggctcgcgcg gcgggcatcg agcaccttgt
     7861 cttcgtgacc ggccgcaaca aggccgtgat cgaggatcat ttcgacagcg cctacgagct
     7921 ggagcgcacc ctcgccgagc gcgggaagac caaggacctc gagcagctcc agcgcgaatt
     7981 gcccgcggcc ggcgcgacga gcttcacgcg ccagcaggca ccgctgggac ttggacacgc
     8041 cgtatggtgc gcgcgcgaga tcgtggggga cgagccgttc gcgctcctgc tgcccgacat
     8101 gctgcaccac ggcgacccgc cgtgcctcgc cgagatgatg gcggcctacg cggagcacgg
     8161 aggcaatcac gtcgccgttg ccgctgtgcc agaggaccag acgcatctct acggcatcgt
     8221 cggcgtggag gacagcacgg ccaaggtttc ccgcatcacc tcgatggtcg agaaaccgcc
     8281 gcccggcacg gcgccatcca atctgcacat atcggggcgc tatatcctgc agccggagat
     8341 cttcggactg ctcgaaaagc aggagcgcgg cgccggcgga gaaattcagc tcaccgattc
     8401 catgctgaag ctcgccgagc agcagccgtt ctatgccgtc cgcttcgacg ggaagatcta
     8461 cgactgcggc tcgaagatcg gttttctggc ggcgaacgtc gcatacgcgc tcgagcgtga
     8521 tgatctcggc ccagcgctgc gcgaggagct gaagcagctc ctttgactat cggccctcgt
     8581 gagggaatgc cgggttcaaa atgacatgga gcggccgcac accagcgcgg ctgcatcccg
     8641 ggccgtacac gttgtacggc ccatctcctt gctttcctga ccttacgaaa ggctatatcc
     8701 aacgcccgga ataacgaagt attgcaattt gtttcggttc atcaggctcc tgtttcggct
     8761 gtgtcaccgt atggtttcag cctgtccctg catttctctg attccacgat attttctcta
     8821 ttgcgccacg gagccgtcac accggtcgca caactaggcg tcggcggttg cgtgatgtaa
     8881 ccaccgaggg aggtgcttga gtacgcaggg cagtggctct cgacgccggc tgcccgctgc
     8941 aaggagcgcc agtatgcgtt ttcgattgat gctgggtgct gcgtgcgcct gctttgccgc
     9001 cttgcccgcg ctggccgcag atctgggcgg gccccgaaga ttgccgccgc cggccaattt
     9061 cgacgagcca ttcattcccg acagcaagcc acctctgtgg caggggctct actggggtct
     9121 gagcggcggc tacggttggg gcagctctga tgcttccttc gatccaggcg taaactccgc
     9181 cagcacggac ccggagggat ttctggccag cgccacggtc ggctacaaca tgctgctcgg
     9241 ctcgagcctg ctcgtcggcg ttgaaggtga cctcggcgtc atggatatca gcgccgacga
     9301 tgaagatgtc ggaggcgcgc tttaccgaac cagcttcgga ccattttggg gcaccctgag
     9361 aggccgggcc ggcatcccca tagggccgtt gctgctttat ggcaccggtg gtctcgcctt
     9421 cgcgagcctc gatgaggtct caatcgaaac cggagatgag aaggcgagcg acagcgagct
     9481 tcgtgctggc tgggtcgccg gtggcggcgc cgagttcgcc ttcagcgaat cgacgacggc
     9541 caaagtcgaa tatctccata tggaattcga cagcacggat gggttgacct cggacggaag
     9601 cttctccttc gacaacaagc tcgacgtcgt ccgcgccggc ttgaacttcc gcttctgatt
     9661 ttttctcttc tccggcgggg ccgccgatgt cttgggcggc ccctgtcagg cgacacagac
     9721 ctcgccgatg ggctcactat ggatcgaaag atcccggatc gtcggattac gccccgataa
     9781 cggattgtcc ggatcccagg cgaccttcac aatctcgaaa cgggccgaga gcgcgtcgta
     9841 gatcagcagc cgtccggcaa ggctatcgcc catctcgagg atctccttga gaacttccgt
     9901 ggcctgcagc gtgccaatga cgccgacgac cgagcccagc acgccgattt cgctgcaggg
     9961 tgccacggtc cccggcggcg gcgcctcggg gaaaatgcag cgatagctgg gccagggctc
    10021 gccctcggca ttcttcagat gcggcttgag gaccgtgaca tagccgtcga acgggcccac
    10081 tgcggcaaac accagcgtgc gcttggcgag atagcacgcg tcgctcgtca ggtagcgcgt
    10141 cgcgaagttg tccgagccat cggcaacgat gtcgtaccgg ctgatgatgt cgatcgcgtt
    10201 ctccgccgtg aggcggcagt tgtgcgtctc gaccgtcacg tgcggattga gccgctcgac
    10261 cgcgagcttt gcgctctcca ccttcggcgt gcccacctgt gccgtttcgt gcacgatctg
    10321 acgttgcagg ttgtcgagcg agacgcggtc atcgtcgatg acgccgatcg tgccgacgcc
    10381 ggccgctgcg aggtacatga tgaccggcga gccgaggccc ccggcgccta cgaccagcac
    10441 gcgcgcgtcc ttcagcttct gctgcccctg cccgccgacc tcgcgcagca cgaggtgacg
    10501 cttgtagcgc tcgatctcct ggggcgtcag tttcgtcatc gtacgcgctc cttgcgagat
    10561 gcgggttata ggggccaaga ggaccgcaca cccgatggct cgcaaagtca accccggccc
    10621 gaaatcgacg atttgcgact attcccgcgg cttttcgggc gactttttcg cgcgagtcga
    10681 agcggctgat ttaatcggta tacgtggttg aggcgccaga gccaccccag ccgttcccgt
    10741 cgagccgaac ccgcctgcgc cgcgccgtgt atcattcagc gtggctacct cgaccagatc
    10801 ggcacgcgcg acccgctgga agacgagctg agcgatccgc tcgccgcgct ggatctccca
    10861 cggcgcaccg ccgaggttga tgagcaggac cttcacctcg ccgcggtaat cactgtcgat
    10921 cgtgccgggg ctgttcagca ccgtgatgcc gtggcgcaag gcgagacccg atcgcggccg
    10981 gacctgcccc tcaagcccgc gcggcaactc catgacgagg cccgtcggga tgagctgtct
    11041 ctcgccgggc gccagcacaa tgggggcgtc ttcctccact gcggccaaca ggtccatgcc
    11101 agccgcttcg tccgactgat aggcgggcag cggaaggccc tcggcgtggg gcagccgcac
    11161 gatccggacc tcaatggtgt cgtctggggc acggggcatc acacggagac ctgcgtctgg
    11221 gccagatgct ccgcggcgcg ctcgaccaag cggcgggcca cctcggcctt gtccatcgcc
    11281 ggccagcttt cgacggtccg ggctgtgatc aagtgcacgg tgttgcggtc accgcccatg
    11341 gcgccgcctt cggccgaaac atcgttggcc acgatccagt cgcagccttt cgccttgcgc
    11401 ttcttcttgg cctgctcgac gacattctcg gtttcagcag caaaccctat gaccagggcc
    11461 ggacgtcgct ccccccgcgg agaccgcgag gcgatcgtct tcaagacgtc ggggttctcg
    11521 acgaactcga gggccggtgc cgccgccgac ttgtccttct tgatcttctg cgaagctgca
    11581 ttggcaacgc gccagtccgc caccgcagcg gcgaaaatgg caatgtcggc gggcagcgca
    11641 gcctcgacag ctgccagcat ttcccgcgcc gtctctacgt gaacgacgtc gacgcccggt
    11701 ggatcggcaa tcgcgactgg ccccgaaacg agcgtcacac gggcgccgag ctctgccgcg
    11761 gcccgggcga tggcatggcc ctgcttgccc gaggagcggt tggcgatgta gcggacggga
    11821 tcgatcggct cgtgggtcgg cccgctggtg acgagcacgt ggcgaccgga aagcggcccc
    11881 ttcgctgccg gccgccgcaa cagcgcttcg accgcggcaa cgatctccag cggctctgcc
    11941 atgcggccgg gacccgcctc cccctgctcc gccatctccc cagtgttcgg gccgacgaaa
    12001 tggatgccgt cggcctgcag catcgcgaga ttgcgctgcg tcgccgggtg cagccacatc
    12061 cgcgggttca ttgccggtgc ggcgagcaca ggcttgtctg tcgcaaggag aaccgcggtg
    12121 gcgagatcat ccgcgtgccc gcccgccatc ttcgccagca ggcttgccgt tgccggagcg
    12181 agcacgatca aatccgcctc ccggctgagc cttatgtgtc cgacatcgtg ctcgtcctcg
    12241 cgatcgaaca gatcggagaa gacccggtcg cccgaaatgg ctccgaccgc caacggcgtg
    12301 acgaactgct gtgcggcagc ggtcatgacg gtccgcaccc gcatcccgcg ctcgcgcagg
    12361 cgccggatga ggtcgaggca tttgtaagcg gcaatcccgc cgccgatgat cagaagaatt
    12421 cgcttgtcgg tcacgtcgat aaccgctggc ttgcagagcc gcgaaaccaa gcacatggat
    12481 ggcggttgct ggctaacatc ggtagaaaca gcccatggct tcgcctttcc gcgccggcat
    12541 ccgcctgaaa acttctctcg ccgcttgaaa ttacctgtac tatagttcga cgacggcggg
    12601 gcgccgaccg gaaacggatg gtgtagatgg gatagagcgg tccagaactg atgtccggag
    12661 tggcatagcg catttgctgc gagcaggcca tccggcaagc agacctacgc ccgaggtggg
    12721 actgtcgagg tgtccgtccc tcgaccctcc cgcgtcgcga acctgttgca tgtcgtcgtt
    12781 ggcagtgcag ggttgaggcg gcatggagct cgtcacccat ttccggcgca tggcgcgcaa
    12841 caatgcttgg tcgaactggc gattgctcga tgcctgtagt cgcctgagcc ccgaggaatt
    12901 ccgggccccg cgcacgagct tcttcccttc gatccaggcg acgctcgacc acatcctgtg
    12961 tgtcgacctc tattacctgg atgccttgga aagcggaaag cttggctatg cgtatttcga
    13021 gtctcgccgg ccgattgagg agttcgccgt ccttcgcacc gaccagatcg ctacggatca
    13081 acggctgatc ggcttctgcg atcgcctcac ccccgatgat ctcagacgca ttatcggcat
    13141 cgaccgcggc ccgaagggaa tcgactacga gaccgtgggc gatacgctct cccacctctt
    13201 cgtgcaccag atccatcatc gcggacaggt gcacgccatg ctggcgggaa caaacgtcaa
    13261 gccgccacag ctcgacgagt atttcctcca gtacgaccgg gacatccgcg cgcctgatct
    13321 ggcagccctc gagttggaag gcccggaatc gctcgtctga taccggctga cccgatacga
    13381 accgactgaa cttgaacggc gcggtagcgt gtttcggatc gagctcgcgt tccgatcgcc
    13441 accgcgccgc gcgccggata cgcggtaccg aattcacggc gcacctccat ctaagcccaa
    13501 aaggttgcgg gatcgcaaac gcttcgcgct ccggtcgcga gcaagtgtcg cggtagcagg
    13561 gggagtcaat cgcagaagct aagccgcctg cctggcatgg gattccagcc aacgcggcat
    13621 gaaggtatcg agccagcggc ggacattggg cccataggcc agatgattgg aaagctgcga
    13681 cggcctgtcc tgcgcacctg gaagggtgag gcgttccgga tggcttccgc tccatcgctt
    13741 gaccatggcg tacgtgatct ccgggtgaaa ctgcacgccg acggccgtct cgccgtagac
    13801 gacggcctgg ttctcgaagg gcccgtcggc cgtcgccagg acggtcgcac cgtgtggccg
    13861 gtcgaaaccc tcgcgatgcc attggtagac gcacctcggc cagtcgccga agctgcgggc
    13921 gtgcggtgtc ggccggatcg ggtggtagcc gatctcgacg cgccgctcgg ggtgctcata
    13981 gacgcgagcg ccgagctgcc gcgcaagcat ctgcgccccg aggcagatgc cgaggaacgg
    14041 cttgcgctcc ttgagcgcca caccgatcca ctcggtctcg agcctgatgt agtcatccgg
    14101 gtcgttggcg ctcatcggtc ccccgaagat gatcgcaccc gcatgatgct ccagcgtctc
    14161 tggcagcgga tcgccgaaac gcggccgacg gatgtcgagc gtatagccgc gcatcctgag
    14221 ccagtgtccg acgtggccag gattggaatg gagctggtga aggatgacga ggaccgtttt
    14281 gcattcctgc ggcggtgcgg ccggcttccg acccgtcccc agggtgatcg cgccgccttc
    14341 cgggtccgtt gttctaaagt ccagcattct tcaatagttc cctcggcttc agcagccagc
    14401 caacaatcgg taacagaacc gggtggggga tcaagcgcat ggcgatcgcc agcaacggcc
    14461 ccagcacgcc cggggtgatc actgtccgtc cccactcgaa ccaacgcacc gcagtaccgg
    14521 cagccgtcga cggccctggc gccgggatga gccagcgata gagcgcgtgc tcagctccca
    14581 tgcgccgatg gaagcgcgtc cgtatcggac ccggcgcgaa cacggcgatc cgtgcaccgc
    14641 gcccgcccga ctcccacgcg acggcttccg tgagcgagat cacataggct ttgctcgcat
    14701 agtaagccgc ctgatatggg cccggcgcat aaccgccaag cgatccgacg ttcagcacgc
    14761 cgccacggcc gcgcacgcac atatccggca ggaaatgccg catgaggagt gaaacggcgc
    14821 ggatattgag ctcgaccagt gccgcgatgc ggtctcccgg ttgctctata tagtcgccgc
    14881 tcaagcccat gccagcgttg ttcaccagca catcgacgta gaagccacgc cccctgagct
    14941 cggtctcgat cacctcggcg gcatcaggcc gggtcacgtc gagcgaaatg gtcagaacgg
    15001 cggttccgaa ctcaccccgg atggccgcgg ccgcctcctc cagtctgtcc ggccgtcgcg
    15061 caacgagcac cacggtgctg ccgcggcggg caaaccggcg ggcgatctcg aacccgatcc
    15121 cctccgagcc gccggtgacg acaacagcag gcctgaggtc ggcccattgc gccatcgcct
    15181 cagggttggg gcgccaccgg cggcgaaccc acaagtcggc cagtcgcgtg atcatgctcc
    15241 gcccgccagc cgctcccagt gggtcatccc tgcaccagcc gtcactcggc cgcgacaggc
    15301 cgcaagacgc cggcgcgcgg agctcgcctg cggccagttg tggccgtcac acgctgccga
    15361 acttccggtc gtcgctgcat ctggcctcgg ctcggcccaa atcgaagctc gatggtctgc
    15421 cggatatacc gggcgagctc ccgcgtgatc tccgttgcac ccgccggcag atagaggttg
    15481 accctgaagt ccggcaacgg cggcagaccc gagccggcac cgaggatctt caggtagtcg
    15541 gggaccgacg tgcggatgag cggcgcgacc gccaatcccg ccttcaccgt cgcataaagc
    15601 ggctccatgt tgcagtcctg gctgaccggg cgccagtcga tctgcgctgc atccagcgcc
    15661 ttgatgacct ccggccggaa gcggcacagc ttggtcccga gcgaaaccgg cagcggccgc
    15721 tgcaggtgcg tgttgcttcc agggcctccg acccagacga gccggtccga aagcagcgtc
    15781 tcccccgccg ggccgcaatc gatctccgtc gtcaggatca aatcgacctt ccggcccgcc
    15841 agatcctcga tcagagaggc tgacgccttg cagatcagcg agacattgat gcgcggccag
    15901 gcttggtgga agcgttgcag caccggcgga atgaggctgg tcacgatgtc gggcggcacg
    15961 ccgaaccgga cctcgccttc gaactgtggc gtggtcatcg cgccccaggt ggcatcattc
    16021 agcgcgacga ggcgctgtgc actgccgaag aggcgttctc ccgccggcga cagcgtgaac
    16081 cgcttgcgct cacgaaggaa gagctccgtg ccgaacagct cttccaggcg cttgatctgg
    16141 agactgacgg ctgcctgggt gcgattgaga atgcgagctg ccgtggtgac gcctcccgtc
    16201 tccacaaccg ccagaaacgc acgcaacaga gcgatgtcga tatcccgtgt catcggaacc
    16261 tcgggggttc gagagggcgt cgccgctaca taatcgatcg tcatgcatca cataaacatc
    16321 attcgtttcc gttatgcaag cacatgtgat agtcagcgaa ctgcaacggt gccgatgcgg
    16381 cccggcgcgc aataaaaatg gagagttgag atgacgagca cgacgcagag cgcatggcag
    16441 gcaagccatg gccaagcgat accgcgcact ggccgttttc tgcggatctt cgacaggctg
    16501 tggctggcct acaaggtcta ccgtgagcgc caagccctcc tggcgctcag tgattatgag
    16561 ctcagagaca tcggcctcag ccgggccgat gcccgccgtg aggcgaatcg tccactgtgg
    16621 gatctgccag aaatacgctg acgcgctctg tgcgtccggg ggcggagccg gaagctccgc
    16681 ccctcgtctg cgccccacgt gccgccgcgg gcatgaccgg caccgagccg ccagcgcgag
    16741 cagccgttcc tgcgagcgac gtggaatctg gtcgacgcgc tcgtcggtca cgaccagtct
    16801 gacgtcccca gcacgcacca acggtcatca tcgggatgca ggcgtctgcc gtcgtagggc
    16861 cggccatgac cacgtcgtcg cagttgcttt ggcttgaacc tggcgaaggc cgcatcagga
    16921 ttgtggtcgg ggcggcgcac tgggctcagt tccggaatcc tgcggatcgt gagggaagcc
    16981 cttgcacttc catgcggcag gcattagtag tgcagcccta ctgctcgccg cccgccgcgg
    17041 cctcgcagcc ttggcgaaag caccggtagc tcagctggat agagcaccag actacgaatc
    17101 tgggggtcgt gggttcgaat cctgcccggt gcgccattcc cgaacaaaag agagaaccga
    17161 cggcgagctt gtgccaagcc cccccgctgc agtgcgggca agcacggcct tcgagccgct
    17221 gatcctgatt tctcgatcat cgacagtaac ttcgttgagg atcgggcgcg cgta
//
LOCUS       DASVDU010000219        11056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_727049, whole genome shotgun sequence.
ACCESSION   DASVDU010000219 DASVDU010000000
VERSION     DASVDU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11056
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727049"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..3004
                     /gene="metH"
                     /locus_tag="VF226_14060"
     CDS             311..3004
                     /gene="metH"
                     /locus_tag="VF226_14060"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX7075160.1"
                     /translation="MEMTTNAAATFINIGERTNVTGSAKFRKLIEAGDYQAAVAIALS
                     QVENGAQIIDVNMDEGLLDSEQAMRTFLNLIATEPAIARVPVMIDSSKWSVIEAGLKC
                     VQGKAIVNSISLKEGEGPFLEQARKILRYGAAVVVMAFDEQGQADTVERKVAICERAY
                     KLLTEKVGFPPEDIIFDPNIFAVATGMEEHNNYGVAFIEATRQIRQKMPLVHISGGVS
                     NLSFAFRGNEPVRQAMHSVFLYHAIQAGMDMGIVNAGQLTVYDDIPPELRELCEDVIL
                     NRRPDATDRLLEAAPKFKGGGAKKVEQDLSWRDAPVEERLKHALVHGITEFIEVDTEE
                     ARQKAARPLDVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAYLQPFLEAE
                     KKAMGLDALPAAGKVVMATVKGDVHDIGKSIVGVVLECNNYEVIDLGVMVPAAKILEA
                     ARKHQADMIGLSGLITPSLDEMCHVAAEMEREGFDCPLLIGGATTSRVHTAVKINPNY
                     KRGATIHVLDASRAVGVVSTLLSQSERDSYVAKLRAEYEKIREQHLRGEARKNRVSLQ
                     AARANRHACDWSSYTPPVPRFLGTRVFQDYDLRELIPCIDWSPFFAAWQMNGRFPAIL
                     DDNKIGAEARKLYADAKALLQRIVEERWLTANAVVGFWPANAIGDDIELYTDETRTER
                     LAMFYTLRQQIARDPSRKRPHAALADFIAPRETGLADYIGGFAVTAGLGEEEALANRI
                     APHDDYSRIMLKALADRLAEAFAERMHQRVRMELWGYAPDERLTNEELIAEKYRGIRP
                     APGYPAQPDHTEKGTLFRLLDATRATGIKLTESYAMWPGSAVSGLYFSHPESYYFGVG
                     KVERDQVEDYARRKGWPVSEAERWLSSVLNYLPDVAGSEAA"
     gene            3077..3412
                     /locus_tag="VF226_14065"
     CDS             3077..3412
                     /locus_tag="VF226_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075161.1"
                     /translation="MLGRLVLILLQLAIGWFGAPQVLRYVPAPGDAQVFVYAVAAAII
                     IWVVGLIGAQVLKDVATPSAGTLVAALIGGVIGAAIVAFKLNQMIPVNVPPNLWPLGL
                     AIVGYALRK"
     gene            4192..5175
                     /locus_tag="VF226_14070"
     CDS             4192..5175
                     /locus_tag="VF226_14070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEX7075162.1"
                     /translation="MQFKALLLTKSADGQRAEWTELSDADLMPGDVLIRVTHSTINYK
                     DGLALTGKAPVVRRWPMIPGIDFAGTVEQSSHPDIKVGDNVILNGWGAGETHYGGYAQ
                     YARVSGDWLVKRPAEFTAAQAMAIGTAGYTAMLAVMALEDHGVTPDRGPVLVTGAAGG
                     VGSIAVTLLAKLGYEVIASTGRREEEAYLKGLGASEVIDRADLTGPAKPLGRERWAGA
                     VDVAGSHTLANVLSMTRYGGTVAACGLAQGLDLPGSVAPFILRGVTLAGIDSVMCPKP
                     RRVAAWRRLARDLDRDKLDALSTTRPLSDVLTLAPEILAGKIRGRVVLEVG"
     gene            complement(5197..5994)
                     /locus_tag="VF226_14075"
     CDS             complement(5197..5994)
                     /locus_tag="VF226_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7075163.1"
                     /translation="MVDDVKGPQLRIDNRGSIRWLIIDNPARLNAFTTSMWAALPRLI
                     REAEEDGDVRAVVLTGAGDRAFSAGADISEFETARTAEKAAEYDALNNAAFEALSRSA
                     KPTIAAIQGYCMGGGLELAICCDLRVASDTAQFAVPAAKLGIGYNPRWIRPLLGVVSS
                     ARARELLYTGRRFSAADALAMGLVNSVHAPGELIAAATALAEEIAANAPLSVLAAKRA
                     IDEFISHPENPDLPMLDRYVDACFSSEDYVEGRRAFMAKRKPQFRGR"
     gene            6196..7035
                     /gene="cysE"
                     /locus_tag="VF226_14080"
     CDS             6196..7035
                     /gene="cysE"
                     /locus_tag="VF226_14080"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX7075164.1"
                     /translation="MAPTSRQAAQSRIAHVDPIWAEVRREAEEAIRAEPALGGFIFAT
                     VMGHERLEDAVCHRLAQRLNHSDVDAGLIGQMFQDVLTRCTELGRAFRADLAAVYDRD
                     PACSRYIEPLLYFKGFHALVTHRFAHELWKEGRRDFALYLQSQSSRIFGVDIHPAARI
                     GIGIMIDHATGVVIGETATVGDNTSLLHGVTLGGSGKEGGDRHPKIGANVMIGAGAKV
                     LGNIKVGDCSRIAAGSVVLKEVPPNTTVAGVPAKVVGPAGCPEPARTMDQRLSPDDCG
                     CGN"
     gene            7080..7292
                     /locus_tag="VF226_14085"
     CDS             7080..7292
                     /locus_tag="VF226_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEX7075165.1"
                     /translation="MKKDVPRVQKYLRKLFRTDQLRVVPHARKKDMAEVFIGDEFIAP
                     LYREEEDGEVSYQLQVVILDVDLEEN"
     gene            7307..7852
                     /locus_tag="VF226_14090"
     CDS             7307..7852
                     /locus_tag="VF226_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX7075166.1"
                     /translation="MGLFKLDDVSVTTPGEGRYWVAANATVLGNVHIGEDASVWFNTV
                     IRGDNEPIQIGTGSNIQDGCVLHTDPGFPMVIGPDCTIGHMVMLHGCKIGRGSLIGIG
                     SIILNGAVIGEECLIGANTLIPEGKEIPARSVVMGSPGKVVRQVTEADLARMQAGAAH
                     YRKNWRRFAAGFAAQSKSLSE"
     gene            complement(8070..9941)
                     /gene="ftsH"
                     /locus_tag="VF226_14095"
     CDS             complement(8070..9941)
                     /gene="ftsH"
                     /locus_tag="VF226_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7075167.1"
                     /translation="MNKQQIWNIGYFIAAFLLLVLFQNMWTTYQNVEPIPYSEFLKLS
                     NEGQIVEVTVTNEQITGKLKQPINGRQYFVTNRVDPTLADELAKKGVTVSGTVQNTFL
                     STLLSWLLPMLIFFGIWIFVFRQFAQRQGLGGLMNVGKSKARVLVERNTGVTFDDVAG
                     VDEAKAELQEIVSFLKDKETYGRLGARIPKGILLVGPPGTGKTLMAKAVAGEAGVPFF
                     SISGSEFVEMFVGVGAARVRDLFEQARKHAPCIIFIDELDALGRARSAGAGYGGGHDE
                     KEQTLNQLLAELDGFDPREGIVILAATNRPEILDPALLRAGRFDRQVVIDRPDKKGRE
                     AILAVHIKKIKVAPSVKLDQIAAITPGFSGADLANLVNEAAIVATRRGANEVTLADFT
                     AAVERIVAGAERKGRLLQPRERKIVAYHEMGHALVAAALPDTDPVHKVSIIPRSIGAL
                     GYTMQRPTDDRYLISRQELKDRMVVLLAGRAAEQLVFGELSTGAADDLVKVTDIARQI
                     VTRFGMTDLLGQAVLERQSTTFLGEQKLAVREKDYSEGTAREIDLAVKDLIDEAYERA
                     LQILAERRSLLDEGAKLLLEKETITPEDFPALKQRPVSGDVRQPEVVLSAGAAGGSG"
     gene            10174..10809
                     /locus_tag="VF226_14100"
     CDS             10174..10809
                     /locus_tag="VF226_14100"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7075168.1"
                     /translation="MPGHTLQKYLTSWPLWAGLLVLAFLWLGPLPAMSRRAFSPHMIL
                     HLAVTSLAAPLIVAGLLRSGVAVRISRQHTLFAVGASIFELAVVWGWHLPELHQAAAL
                     DDTVYVAQQLSFLAAGIAIWWASFAGMTKVAYAIGGLALVLTFVHMAMLGLLIGIAPE
                     LIYEPSICLGAFGFDPLEDQHFGGSLMAVVGGTPYLIAGIFLLNKFISEHG"
BASE COUNT         2017 a         3412 c         3569 g         2058 t
ORIGIN      
        1 acgccggagc acatcaggca cgtccgcgag cacgtcgaga aatacccgcc gcggaaagtg
       61 ccaaagctcg agccgcgaat gcgcctgtcg ggcctcgagc cgtttgtgca cgggtgagcg
      121 gatggaagcc atccgcgtgg agatcgtact cgtcccttcc gtcatggccg cggaggcggc
      181 cctccacgca agtctccaca cctgcgcggc tggctggctt ggattcccgc cttcgcggga
      241 atgacgggtt ggagctaatg ggaagcgcta atttcatcct ctgagcgctt gcgttcggaa
      301 agagagttga atcgaaatga ccaccaacgc agccgccacc ttcatcaaca tcggtgagcg
      361 cacgaacgtt accggatcgg caaagttcag aaagctgatc gaggctgggg attatcaggc
      421 cgcagtcgcc atcgcgctct cccaggtcga gaacggtgct cagatcatcg acgtcaacat
      481 ggacgaaggg ctgctcgatt cagagcaggc catgcggacg ttcttgaatc tcatcgcaac
      541 cgagccggct atcgcgcgcg tgccagtcat gatcgattcg tcgaaatggt cggtgatcga
      601 agcgggcctg aagtgcgtcc agggcaaggc catcgtgaac tcgatcagcc tcaaggaagg
      661 cgaagggccc tttctggagc aggcccgcaa gatcctgcgc tatggcgcgg cggtcgtggt
      721 catggccttc gacgagcagg gccaggctga cacggtcgag cgcaaggtcg cgatctgcga
      781 gcgggcgtat aagctgctga cggagaaagt cggctttccg cccgaggaca tcatcttcga
      841 tccgaacatc ttcgccgtcg ccaccggaat ggaggagcac aacaattacg gtgtggcctt
      901 catcgaggcg acgcggcaga tccggcagaa gatgccgctg gtgcatatct cgggcggcgt
      961 ctccaacctg tccttcgcct tccgcggcaa cgagccggtg cgccaggcga tgcactccgt
     1021 gttcctctat cacgccatcc aggctgggat ggacatggga atcgtcaacg ccggccagct
     1081 cacggtctac gacgacatcc cgccggagct ccgggagctg tgcgaggacg tgatcctgaa
     1141 ccgccggccc gatgcgacgg atcggctgct ggaggcggca ccaaagttca aggggggcgg
     1201 ggcgaagaag gtcgagcagg acctctcctg gcgcgatgcg ccggtcgagg agcggctgaa
     1261 gcacgcactg gtccacggaa tcaccgagtt catcgaagtc gacaccgagg aagcgcgcca
     1321 gaaggcagcc cggcccctcg atgtcatcga aggtcccctg atggctggca tgaacgtcgt
     1381 cggcgacctg ttcggcgcgg gcaagatgtt cctgccgcag gtcgtgaagt ccgcgcgtgt
     1441 gatgaagcag gccgtcgcct atctgcaacc tttcctcgag gccgagaaga aggcgatggg
     1501 gctcgacgcc ctgccggctg ccggcaaggt cgtgatggca accgtcaagg gggacgttca
     1561 cgacatcggc aagagcatcg tcggcgtcgt gctcgagtgc aacaactacg aggtcatcga
     1621 tctcggcgtc atggtgcctg cggccaagat cctcgaggcc gcgcgcaagc atcaggcgga
     1681 catgatcggc ctctcaggcc tgatcacgcc ctctctcgat gaaatgtgcc acgtcgcagc
     1741 cgagatggag cgggagggct tcgactgccc tctgttgatc ggcggcgcaa caaccagtcg
     1801 cgtccacacg gcggtgaaga tcaacccgaa ttacaagcgg ggcgcaacga tccacgtcct
     1861 ggacgcgagc cgcgctgtgg gcgtcgtctc cacgctgctg tcgcagtcgg aaagggacag
     1921 ctacgtcgcg aagctgcgag ccgagtatga gaagatccgt gagcagcacc tgagaggcga
     1981 ggcgcgcaag aatcgtgtgt cgctccaggc ggcccgcgcc aaccgccatg cctgcgactg
     2041 gtcgagctac acgccgccgg tgccgcggtt cctcggcacg cgcgtgttcc aggattacga
     2101 cctcagagag ctgatcccgt gcatcgactg gtcaccgttc ttcgcggcgt ggcagatgaa
     2161 cgggcgtttt ccggccatcc tcgacgacaa caagatcggt gccgaggcgc ggaagctcta
     2221 tgcggatgcg aaggcgctgc tccaacgcat cgtcgaggag cgctggctga cggccaacgc
     2281 cgtcgtcggc ttctggccgg ccaatgccat cggggacgac atcgagctgt atacggatga
     2341 gacgcgtacc gaacggctcg ccatgttcta cacgctgcgc cagcagatcg cccgcgaccc
     2401 gagccgcaaa cgcccgcatg cggcgctcgc cgacttcatt gcgccgcgcg agacggggct
     2461 tgccgattac atcggcggct ttgcggtgac cgcggggctt ggcgaggagg aagccctcgc
     2521 caaccgcatc gcaccgcacg acgactactc gcgcatcatg ctcaaggcgc tggccgaccg
     2581 cttggccgaa gcgttcgcgg agcgcatgca tcagcgcgtg cgcatggagc tgtggggcta
     2641 cgcgcccgac gagcggctga cgaacgagga gctgatcgca gagaagtacc gcggcatccg
     2701 gccggcgccg ggatatcccg cgcagccgga tcacacggaa aagggcacgc tgttcaggct
     2761 gctcgatgcg acccgcgcga ccggcatcaa gcttaccgaa agctacgcca tgtggccggg
     2821 ctcggccgtg agcgggctct atttctcgca tccggagagc tactatttcg gtgtcggcaa
     2881 ggtggagcgc gaccaggtcg aggactatgc gcgccgcaag ggctggccgg tgtccgaggc
     2941 cgagcgctgg ctttcatctg tgctcaacta tctccccgat gtcgctggaa gcgaagcggc
     3001 gtgataagtc tccgttttca tcgtgcgggg atgacggacg tcctcgctcg aaaacaccaa
     3061 agggggaggt gatttcatgc ttggacgtct ggtgcttatt ctgctgcagc tggccatcgg
     3121 ctggttcggc gcgccgcaag tgctgcgtta cgtgcccgct cccggcgatg cgcaggtatt
     3181 tgtctatgcc gtagccgcgg ccatcatcat ctgggtggtc ggcctgattg gcgcccaggt
     3241 cctcaaagat gttgcgacac cgtcggctgg gacgctggtc gcggcgctga tcggtggtgt
     3301 gatcggcgcc gcgatcgtgg ccttcaagct gaaccagatg atacccgtca acgtgccgcc
     3361 gaacctgtgg ccgctcggcc ttgcgattgt cggctacgca ctgagaaaat agcccacata
     3421 gcttttccgc tttgaggtcg gcccgcaggg ccgggtgagg ggctacaaca attgccactt
     3481 ccggaaccct cgtccagccc actcttggct tggtcgcgat tggctctcga atcagaagag
     3541 gcgcggagtt ggaccgcgcc tcttccaata aactccagtt acaatgggtt ctctgagtgc
     3601 cctgaccgtt tcacccctga tttggaatgc ggctgcaggg cctccgctca gatcacaccg
     3661 aacacgatcg cagcgaggaa tccgaacgac atgatcgcgg caagcacgtt cagtctcatc
     3721 ttcagttcca tcgtgcctcc tttgccggtt acccagcggt taggcagctc aggcgatgcc
     3781 tgtgcgattg gccgaatcta atggagtgac tcagcgtgac acaagcgaaa aacgtcgctg
     3841 cgctgcaata gaatgcagtc tcttcacgat ccctaaccgt cccctccctc gattagggta
     3901 gtgccgcaac agtgatgccg agcaatcacg gcgacaaacg ggtagagcca acgccaataa
     3961 cgtttcagtc aactataagt tccggatcac tctcctcgaa tgatcttgag aattaaggaa
     4021 gaagatattg gaacatttgc gagtattgga ttgaagatgg accggcagtc tcgtggtaga
     4081 tgtgcaacag cgggagcacc cccgtagatg tggcggcatt gtggccaaag gagagagaat
     4141 cccgatcgtg gttgaatgat tcgcggcaga cgtgacgtgg aggaagcagt catgcagttc
     4201 aaggccctgt tgctgaccaa gagcgcggac ggccagcgcg ccgaatggac agagctcagt
     4261 gatgcagact tgatgccggg ggacgtgctg attcgggtta cccactccac gatcaattac
     4321 aaggacggcc tcgcactgac tggcaaggca ccggtcgtgc gccgttggcc gatgatcccc
     4381 ggcatcgact tcgccggcac cgtcgagcaa tcgagccacc ccgatatcaa ggtgggcgat
     4441 aacgtcattt tgaacggctg gggcgcagga gagacgcact acggcggcta tgcccagtac
     4501 gcacgcgtga gcggcgactg gctcgtaaag cggccagccg agttcactgc ggcccaggcg
     4561 atggcgatcg ggacggcagg ttacacggcc atgcttgccg tcatggcgct cgaggaccat
     4621 ggcgtgacgc cggaccgcgg tcctgtgctg gtaaccgggg ctgcgggggg cgtgggcagc
     4681 atcgcggtga cgctgctggc caagcttggg tatgaggtga tcgcctcgac gggccgccgc
     4741 gaggaagaag cctacctgaa gggattgggc gcaagcgaag tcatcgacag ggcggatctg
     4801 acgggaccgg caaagcccct cggccgcgag cgctgggcgg gtgccgtcga cgttgccggc
     4861 agccacacgc ttgccaacgt actcagcatg acccgctacg gcggcaccgt tgcggcttgc
     4921 ggtctggcgc aggggctcga cctgccgggc agcgtcgcgc ctttcatctt gcgcggcgtc
     4981 acgctggccg gcatcgatag cgtgatgtgc ccgaagcccc gccgcgtcgc cgcctggcga
     5041 cggctcgccc gcgatctcga tcgtgacaag ctcgatgcgc tcagcacaac gcgcccgttg
     5101 agtgacgtgc tgacgctggc gcccgagatc ctggccggca agatcagagg ccgggttgtc
     5161 ctagaagtcg gctaagcggt cagcgggctc cggtcctcag cggccgcgga actgcggctt
     5221 gcgcttcgcc atgaaggctc ggcggccctc gacgtagtcc tcgctcgaaa agcatgcatc
     5281 gacgtaccgg tcgagcatcg gcaggtccgg gttctccgga tggctgatga actcgtcgat
     5341 ggctcgcttg gcggccagca ccgaaagcgg cgcgttggca gcgatttcct cggcgagcgc
     5401 ggtcgcggcg gcaatcagct cgccgggcgc atgcacgctg ttgacgagcc ccatggcgag
     5461 cgcatccgcg gcactgaagc ggcgcccggt gtagagcagc tcgcgggcgc gcgctgagct
     5521 caccacaccc agcagcggcc ggatccagcg tgggttgtag ccgatgccga gcttcgcggc
     5581 aggcacggcg aactgggccg tgtcggaggc gacccgcaga tcgcagcaga tcgccagctc
     5641 caatccgccg cccatgcagt agccctgtat ggcggcgatg gtcggcttgg ccgagcgcga
     5701 gagggcctcg aaggcagcat tgttgagagc atcgtattcg gccgccttct cggccgtgcg
     5761 ggccgtctcg aactccgaga tgtccgcccc ggccgagaat gccctgtcgc cggcgcccgt
     5821 caggaccacg gcgcggacgt cgccatcctc ttcggcttcc cggatcaagc gcggcagggc
     5881 tgcccacatc gacgtggtga acgcattgag gcgtgctgga ttgtctatga tcagccaccg
     5941 gatgctgccc cgattatcta tccggagctg gggccctttc acgtcgtcta ccatctggtc
     6001 ttgcctctcg ccatcgctat gcccaggtgt tttagtcgcg gctggcgggc cggtgcaaac
     6061 cttgggccat ggtcagtgcg cttgggtcgc aaggcgccgc ccgcccagct cgaacattga
     6121 ctatgggaaa agcgctcgtt agggctgagt gggcaaggtg gccggcggaa aaacaatcct
     6181 ggtaggagat cgatcatggc accgacgtcc cgacaggcag ctcagagcag aatagcccat
     6241 gtcgatccga tctgggccga ggtgcgccgc gaggcggagg aggcgatccg cgccgagccg
     6301 gccctcggcg gcttcatctt tgcgacggtg atgggtcacg agcggttgga ggacgccgtc
     6361 tgccatcgtc ttgcccagcg cctcaatcac tccgacgtcg atgccgggct catcgggcag
     6421 atgtttcagg acgtgctcac ccgctgcacg gagctcggac gggcgttccg cgccgacctc
     6481 gccgccgtct acgatcgtga tccggcctgc agccgctata tcgagccact cctttatttc
     6541 aagggcttcc acgccctcgt cacccatcgc ttcgcccacg agttgtggaa ggaagggcgg
     6601 cgggactttg ccctttacct tcagagccag tcgtcgcgca tcttcggcgt cgatatccac
     6661 ccggcggcgc gcatcggcat cgggatcatg atcgatcacg ccacgggggt cgtgatcggc
     6721 gaaaccgcca ctgtcggcga caacacctcc ttgctgcacg gcgtgaccct cggcggcagc
     6781 ggcaaggagg gcggtgatcg ccatcccaag atcggcgcca acgtgatgat cggggccggg
     6841 gccaaggtgc taggcaacat caaggtcggc gactgctcgc gcattgccgc cggcagcgtc
     6901 gtgctcaagg aagttccgcc gaatacgacc gtcgccggcg ttccggccaa agtggtcgga
     6961 cctgccggtt gcccggagcc tgcccgcacc atggatcagc ggctgagccc cgacgattgc
     7021 ggctgcggga actgacacgg ctcccctcat ccctgcacca gaaaagaagg ccgcacttga
     7081 tgaaaaagga tgttccccgc gtgcagaagt atttgcgcaa gctctttcgc acggatcagc
     7141 ttcgcgtcgt gccgcacgcg cggaagaagg acatggccga ggtgttcatc ggcgatgagt
     7201 tcatcgctcc tctctaccgg gaggaggagg atggcgaggt ttcctaccaa ttgcaggtcg
     7261 tgatcctcga tgtcgatctc gaggaaaatt gagcagggtc ggggaaatgg gacttttcaa
     7321 gctcgatgac gtgagcgtga cgacgccggg cgagggccgc tactgggtgg ctgccaacgc
     7381 cacggtgctt ggcaacgttc acatcgggga ggacgccagc gtctggttca atacggtcat
     7441 ccgcggcgac aacgagccga tccagatcgg caccggctcc aatattcagg acggctgcgt
     7501 gcttcacacg gatccaggct tcccgatggt gatcgggccg gattgcacga tcggccacat
     7561 ggtgatgctg cacggctgca agatcggccg aggcagcctg atcggcatcg ggtcgatcat
     7621 cctcaatggc gccgtgatcg gggaagagtg cctgatcggc gccaatacgt tgattccgga
     7681 aggcaaggag attccggcgc gctcggtcgt gatgggctca ccgggcaagg tcgtccgcca
     7741 ggtgaccgaa gccgatctcg cccgcatgca ggccggagcc gcgcactacc ggaagaattg
     7801 gcgccggttc gcggccgggt ttgccgctca gtccaagagc ctttccgaat gacgtagaaa
     7861 cgaaaggcag cagtggtaac cgcttttacc tctcccatgg ggagaggtcc gccggaaggg
     7921 ccgggtgagg ggttgcaatg atcgccagcg gaacccccct caccccgacc ctctccccgc
     7981 ccgggcagag ggaggttagg cgcaagcttg gctcggcctg tttccgtcca taccggaaat
     8041 gatcctgaac ggcctgttga tcggcgcgct cagccggacc cgccggcggc gccggcgctc
     8101 agcaccacct caggctgacg tacgtcgccg ctgacaggcc gctgcttcag cgcagggaag
     8161 tcctccggcg tgatggtttc cttctcgagc agcaatttcg cgccttcatc gagcaacgac
     8221 cggcgctcgg ccaggatttg cagcgccctc tcgtaagctt cgtcgatcag gtcttttacg
     8281 gccaggtcga tctcacgtgc ggtaccctcg gaatagtctt tctcgcgcac cgcgagcttc
     8341 tgttcgccaa ggaaagtcgt gctttgccgc tcgagcacgg cctggccgag cagatcggtc
     8401 atgccgaagc gcgttacgat ctggcgggcg atgtccgtga ccttgaccag atcgtccgcc
     8461 gcgcctgtgg acagctcgcc gaagacgagc tgctccgctg cccggccggc gagcagcacg
     8521 accatgcggt ctttcagctc ctgccgcgag atcaggtagc gatcatcggt cggccgctgc
     8581 atcgtgtagc cgagcgcacc gatcgaccgc gggatgatcg agaccttgtg cacggggtcc
     8641 gtatcgggaa gagccgccgc gacaagggcg tgacccatct cgtgataggc gacgatcttg
     8701 cgctcgcgcg gttggagcag gcgccccttg cgctcggctc cggccacaat ccgctcaacg
     8761 gcagcggtga aatcggcgag agtgacctcg ttggcgccgc gccgggtcgc gacaatcgcc
     8821 gcttcgttca cgaggttggc aagatcggcg ccgctgaagc cgggcgtgat ggccgcgatc
     8881 tggtcgagct tgacgctggg tgcgaccttg atcttcttga tgtggaccgc aaggatcgct
     8941 tcgcggcctt tcttgtcggg ccggtcgatg acgacctggc ggtcgaagcg gccggcacgc
     9001 agaagtgccg gatcgaggat ctcgggccgg ttggtggctg ccagaatgac gatcccttca
     9061 cgcgggtcga agccgtcgag ctcagccagt aactggttga gagtctgctc cttctcgtcg
     9121 tgcccaccac catagcctgc tccagcgctg cgtgcgcggc cgagcgcatc gagctcatcg
     9181 atgaaaatga tgcacggggc gtgcttccgg gcctgctcga acagatcacg gacccgggcg
     9241 gcacccacgc cgacgaacat ctcgacgaac tcggagcccg aaatcgaaaa gaacggtacc
     9301 cctgcctcgc cggccaccgc cttggccatc agggtcttgc cggttccggg cggaccgacc
     9361 agcagaatac ccttggggat gcgggctccg agccgcccgt atgtctcctt gtccttcaga
     9421 aacgacacga tctcctgcag ctcggctttg gcttcgtcca cgcctgccac gtcgtcgaat
     9481 gtgacgcctg tgttccgctc aaccagcacc cgcgccttcg acttgcccac attcatcagg
     9541 ccgccgagac cttgtctctg ggcaaactgg cggaagacga agatccagat gccgaagaag
     9601 atgagcatcg ggaggagcca cgagagcaat gtcgaaagga acgtgttctg caccgttccg
     9661 gagacagtca cgcccttctt cgcaagctcg tctgccaggg tcgggtcgac acggttggtt
     9721 acgaaatatt ggcggccgtt gatcggctgc ttgagctttc ccgtgatctg ctcattggtg
     9781 acggttacct cgacaatctg cccctcgttc gacagcttca ggaactcgct gtaggggatg
     9841 ggctcgacgt tctgataggt cgtccacatg ttctggaaca gcaccagcag caggaatgcg
     9901 gcaatgaagt atccgatgtt ccagatttgc tgcttgttca tcgctactcc cgcgtgaatt
     9961 tgtgctggtg aaaatgcctc ggacgatgcc agcactatga tttcgtgttc gatgcgccgc
    10021 gagacaagcc tgcgccaggg tctgcaacca aggcgtctac ggtcgggtta acgcttgtgc
    10081 tggggatgcg ccctgctggc gcgcgccacc tttagtgagc ctacggcggg tcgtggccgg
    10141 tccggctgaa gatctgagtg gatatgagaa aaagtgcccg gtcacacgct ccagaaatat
    10201 ctcacatcct ggccgctctg ggcggggctg ctggtgctgg ctttcctttg gctcgggcca
    10261 ttgccggcga tgagccgccg agccttctcg ccccacatga tcttgcattt ggccgttacc
    10321 agcctcgccg cgccgctgat cgttgccggg ctgttgcgtt ccggagtggc cgtgcgcatt
    10381 agccgtcaac acactctctt tgcggtgggc gcttccattt tcgagctcgc cgttgtctgg
    10441 ggctggcacc ttcccgagct gcaccaggcg gcagcgctcg atgacaccgt ctacgtggca
    10501 cagcaattga gctttcttgc cgcgggaatt gcgatctggt gggccagctt cgccgggatg
    10561 accaaagtcg cctatgcgat cggtggattg gctctggtcc tgacgttcgt gcacatggca
    10621 atgctgggcc ttctgatcgg gatcgcgcca gagctcattt atgaaccgtc catctgcctt
    10681 ggcgccttcg gcttcgatcc gttggaggac cagcacttcg gcggatcttt gatggccgtc
    10741 gtcggaggaa cgccttattt gatcgccggc atttttcttc tgaacaagtt catttcagag
    10801 cacggatgag acgcttacaa tcatctttgt tcttccctat gcaacgcatt tgtcagctgg
    10861 tttccggcaa accggtagat ttcttcctcc tggacgaaat gcagccggta tggccgcgag
    10921 acatggagga ggagccgcat gatcttgtgg cattccgtgt cgtcggggcc cggtcgcggt
    10981 attcgaccaa ggtttcagcg caaaagacat cggctgggtc ctgtggcccg gaaccctgat
    11041 cgccaaaggc attctc
//
LOCUS       DASVDU010000220         5900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_692677, whole genome shotgun sequence.
ACCESSION   DASVDU010000220 DASVDU010000000
VERSION     DASVDU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692677"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2418
                     /gene="fdhF"
                     /locus_tag="VF226_14105"
     CDS             <1..2418
                     /gene="fdhF"
                     /locus_tag="VF226_14105"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX7075169.1"
                     /translation="QTTSRFPRGDRPAPDNSHAAIAVNLDACIKCGLCVRACREVQSN
                     DVIGMAYRGHNALPVFDFDVGMGESTCVGCGECVQACPTGALMEANLLDETGKRVAFE
                     DGSVDTVCPYCGVGCQIKVNVKDGKILYVDGRDGPANNNRLCVKGRFGFDYIHHPDRL
                     TKPLIRREDAPKSGDIVVDPRNPWNVFREATWEEALEKAASGLKRIRDRDGGKALAGF
                     GSAKGSNEEAYLFQKLVRQGFGTNNVDHCTRLCHASSVAALMEGLNSGAVTAPFTAAN
                     DADCIIVIGARPAENHPVAATYLKNAAKRGAKLIVMDPRGQKQGLARYASHVLQFKPG
                     RDVALLNAMLNVIVTEGMADVQYVQAHVDGYEDLKARIVDFTPEKMEPVCGIPAATIR
                     EVARLYARSERSIIFWGMGVSQHTHGTDNARCLIALALVTGQIGRPGTGLHPLRGQNN
                     VQGASDAGLIPMVFADYKSVRDPKVRGMYEDLWGTKLDPEPGLTVVEIMHAIHADEIK
                     GMYILGENPAMSDPDAAHAREALAHLEHLVVQDIFLTETAWHADVVLPASAHAEKWGT
                     YTNTNRQVQIGRPVVNPPGEARQDWELIQEIARRIGLNWNYSHVSEVFAEMTKVMPSL
                     ANITWERLLAEGSVTYPCDAPDKPGNEIIFASSFPTASGRARVVPAGLEPPDELPDEE
                     YPFVLTTGRLLEHWHTGAITRRASKLDALEPEAIAGLNPREMDRLGIRPGQFIRVATR
                     RGQVVLKARADRDVAEGMVFIPFCFTEAAANLLTNPQLDPYGKIPEFKFCAARVEAME
                     EKAEAAE"
     gene            complement(2566..3291)
                     /locus_tag="VF226_14110"
     CDS             complement(2566..3291)
                     /locus_tag="VF226_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075170.1"
                     /translation="MRRKEIATIGALAVLVSGTAFAHEISAPANPETPAAFDITRADA
                     TTDGRLATFIMEVRGAAGSVKPTPTGQLQGAKVEAYVWPTRLDPETVGFAPKSGILAL
                     AVTAHPDFDDTPLFDENADGILDNDGANWHSHWVVLGEDAACSGGLKVLDISPGQDAL
                     PLTAPMLPIALDSPGMSPVLDGSRVRLTVPVKGAENVAFDAVTAELQVHPEEKGTPFL
                     CVVGVHKTASEDLSYPGRITRER"
     gene            complement(3288..3989)
                     /locus_tag="VF226_14115"
     CDS             complement(3288..3989)
                     /locus_tag="VF226_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX7075171.1"
                     /translation="MSDDTPPIELDTVTQSISERVSTGLAKISLALKTKAWRGAAPAR
                     LTPTQGQVLVLLRSAPRGLRLDEVARALGVTAPTASDSVAALVSKGLVVRGRSPDNPR
                     AVSLTLSSEGAALAEQVSDWPDFLLRAVDTLDEAEQTAFLRALVKIIRALQVSGDIPP
                     QRMCVSCRYFRPNVHDDPERPHHCAFVDAPFGDRHLRLECQEHEPAPPHEAETSWTRW
                     SGSSASRSMTAGDQA"
     gene            complement(4101..4409)
                     /locus_tag="VF226_14120"
     CDS             complement(4101..4409)
                     /locus_tag="VF226_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075172.1"
                     /translation="MLVIQILRSGGLALALAASTASVAMAHSWYPVECCGGRDCMKVD
                     RVERTPDGGMRMFAGSIQVQVPKGFTQRPSRDNDTHVCVRENGDGSYAPRCVFIPAGV
                     "
     gene            5218..>5900
                     /locus_tag="VF226_14125"
     CDS             5218..>5900
                     /locus_tag="VF226_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075173.1"
                     /translation="MKSAATDLETFLQSTLGTQARRGARVLSGPTIALDVRATAVLKP
                     VLQEIASDIATHGCPDRGGDVAVRWSCDDQGRCVIELSNGNIASAKRADGREAPAGAA
                     PTAFDRPGDGMISCETSEQGARIIVPARYVSDWKQPDPRSVPPAVAPADPLEGRSVLV
                     VEDQLIIALDLEMLLKERGAVAVQLSGSVNDALKSIEAGRPDIAILDVNLGSTTSFPV
                     ACELQRLGIP"
BASE COUNT         1079 a         1833 c         1913 g         1075 t
ORIGIN      
        1 cagacgacca gccgcttccc gcgcggtgat cgtccggcgc ccgacaactc gcatgcggcg
       61 attgcggtca acctcgacgc ctgcatcaag tgcggcctgt gcgtgcgcgc ctgccgcgag
      121 gtgcagtcga acgatgtcat cggcatggcc tatcgcggcc ataatgctct gcccgtgttc
      181 gacttcgacg tcggcatggg tgaatcgacc tgcgtcggct gcggcgagtg tgtccaggcc
      241 tgcccgaccg gcgcgctgat ggaggccaac ctgctcgacg agacgggcaa gcgggtcgcc
      301 ttcgaggatg gatcggtcga caccgtctgc ccgtattgtg gcgtcggctg ccaaatcaag
      361 gtgaacgtca aggacggcaa gatcctctac gtggacggcc gcgacgggcc ggccaacaat
      421 aaccgcttgt gcgtcaaggg ccggttcggc ttcgactaca tccaccatcc ggatcggctg
      481 acgaaaccgc tgatccggcg ggaggacgcg cccaagtccg gcgacatcgt cgtcgacccg
      541 agaaacccgt ggaacgtctt ccgcgaggcg acctgggagg aggcgctgga gaaagcggca
      601 tccggcctga agcgtatccg cgaccgcgac ggcggcaagg cgctcgcggg gttcgggtca
      661 gcgaagggct cgaacgagga ggcgtacctc tttcagaagc tggtgcggca gggcttcggc
      721 acgaacaacg tcgaccactg cacgcggctt tgccacgcgt cttccgtcgc ggcgctgatg
      781 gagggtctca actcgggcgc cgtgacggct ccgttcacgg cggcgaacga tgcggactgc
      841 atcatcgtca tcggcgcgcg gcccgcggaa aaccatccgg ttgctgcaac ttatctgaag
      901 aacgcggcca agcgcggcgc gaaactgatc gtgatggatc cgcgcggcca gaagcaaggt
      961 ctcgcgcgct acgccagcca tgtcctgcag ttcaagcccg gccgcgacgt ggcgctgctc
     1021 aatgcgatgc tgaacgtcat cgtgaccgag ggcatggctg acgttcagta cgtgcaggcc
     1081 catgtcgatg gatacgagga ccttaaagcg cggatcgtag acttcacgcc ggagaaaatg
     1141 gagccggtgt gcggaattcc ggctgctacg atccgcgagg tcgcccggct ctatgcccgc
     1201 tccgagcgct cgatcatctt ctggggcatg ggcgtatccc agcataccca cgggaccgac
     1261 aatgcgcgtt gtctgatcgc gctcgcgctc gtcaccggcc agatcggccg gccgggcact
     1321 gggctgcatc cgctgcgcgg tcagaacaac gtgcagggcg cgtccgatgc cggcctcatc
     1381 ccgatggtgt ttgccgacta caagtccgtc agggatccca aggttcgcgg catgtatgag
     1441 gatttgtggg gtaccaagct cgatccagag cctggcctga cggtggtcga gatcatgcac
     1501 gcgatccatg cggacgagat caagggcatg tacattttgg gcgaaaaccc ggcgatgtcc
     1561 gaccccgacg ccgcccatgc ccgcgaggcg cttgctcatc tcgagcacct cgtggtgcag
     1621 gacatcttcc tgaccgaaac cgcctggcac gccgacgtgg ttctgcccgc ttcggcccat
     1681 gccgagaaat ggggcaccta cacgaacacc aaccggcagg tgcagattgg ccggccggtc
     1741 gtgaacccgc ccggcgaggc gcggcaggac tgggagctga tccaggagat cgcgcgccgc
     1801 atcggcctaa attggaatta cagccacgtc tcggaggttt tcgccgagat gaccaaggtc
     1861 atgccgtcgc tcgcgaacat cacgtgggag cggctgctgg ccgagggatc ggttacctat
     1921 ccctgcgacg cgcccgacaa acctggcaac gagatcatct tcgccagctc attcccgacg
     1981 gcctccggac gcgcgagggt cgtcccggcc ggcctcgagc cgccggatga gctgccggac
     2041 gaggaatacc cgttcgtgct gaccacgggc cggttgctcg agcattggca cacgggcgcc
     2101 ataacccgcc gggcgagcaa gctcgatgcc cttgagccgg aggcgatcgc gggcctgaac
     2161 cccagggaga tggaccgcct cggtattcgt ccgggccagt tcatccgcgt ggcgacccgg
     2221 cgtggccaag tggtcctcaa ggcgcgggcc gatcgcgacg tggcggaagg gatggttttc
     2281 atcccgttct gcttcacgga agcggcggcg aacctgctga ccaatccgca gctcgatcct
     2341 tacggcaaga ttcccgagtt caagttctgc gcggcccggg tcgaggcgat ggaagagaag
     2401 gccgaggcgg cggagtagct gagctacatg gccggacccg gccctgcggg ccaaccccgg
     2461 atcaagtccg gggcaggctc tctccccatg gaagaggtga aggaagcggc tgccgtttgg
     2521 accaattcgg gacggctacc gatcagcgtt cggacaggct gtggctcagc gctcgcgcgt
     2581 gatccgtccc ggatagctga ggtcttcgga tgccgttttg tggacgccca cgacgcagag
     2641 gaacggagtg cctttctcct ccggatggac ctgcagctcg gcggtcactg catcgaacgc
     2701 gacattttcg gcgcccttga ccgggaccgt cagccgaacc ctgctaccgt cgaggaccgg
     2761 gctcatgccg gggctgtcga gggcgatcgg cagcatcggc gccgtgagcg ggagggcatc
     2821 ctgcccaggc gagatatcga ggactttgag gccgcccgag caggccgcat cttcacccag
     2881 aacgacccag tgcgaatgcc aattcgcacc atcgttgtcg aggatgccgt cggcattctc
     2941 atcgaacaat ggcgtatcat cgaagtccgg atgggctgta acggccagcg cgagaatgcc
     3001 agattttggg gcgaatccaa cggtttcggg atcgagccgg gtcggccaaa cgtaggcctc
     3061 caccttggcg ccctgcagct ggcccgtcgg cgtcggcttt accgaacctg cggcaccacg
     3121 gacctccatg atgaacgtgg caaggcgacc gtccgtggtc gcgtccgctc gcgtgatgtc
     3181 gaaggcagcc ggcgtctccg gattggcggg tgcggagatt tcatgtgcga acgcggttcc
     3241 gctgaccaac acggcgagcg cgccgatcgt ggcaatttcc tttcgtctca tgcctgatct
     3301 cctgctgtca tggatctcga tgcgctggaa ccgctccagc gggtccaact cgtctccgcc
     3361 tcgtgagggg gagcgggttc gtgctcctga cactcgaggc gcaggtgacg gtcgccgaag
     3421 ggggcgtcca cgaacgcgca gtgatgcggt cgttcgggat cgtcgtgaac gttgggacgg
     3481 aaatagcggc agctgacgca catccgctgg ggcgggatat cgcccgacac ttgcaatgcg
     3541 cgaatgatct tcaccagagc acggaggaac gcggtctgct cggcttcgtc gagcgtgtcc
     3601 actgcgcgca ggaggaagtc cggccagtcg gaaacctgct ccgccagcgc cgctccctca
     3661 gacgacagcg tgagagagac agcgcggggg ttgtccggcg agcggccgcg caccacgagg
     3721 cccttggaaa ccagcgccgc gacggaatcg cttgccgttg gtgccgttac tccgagggct
     3781 ctcgccactt cgtcgagccg cagacctcga ggggcactcc ggagcaggac gaggacttga
     3841 ccctgcgtgg gcgtgagacg tgctggcgcc gcaccgcgcc aggctttcgt cttcaaggcc
     3901 aggctgatct tggcgagtcc cgtgctcact cgctcggaaa tggactgagt gactgtgtct
     3961 aattcgatcg gcggagtgtc gtcgctcaag acccgttctc ccgtcggatg aacgggttcg
     4021 ttataattag gactcctaat tctgtcaact aaattgtccg aacagctttc tcgcgggggc
     4081 tgtcgcagtt cgggtcagtg tcaaacgccg gccgggatga acacgcatcg cggagcgtag
     4141 ctgccgtcgc cgttctctcg cacgcagaca tgggtgtcat tgtctcgcga cggccgctga
     4201 gtgaatcctt tgggcacctg tacctgaatg gacccggcaa acatgcgcat gccgccgtcg
     4261 ggagttcgtt cgacgcggtc gaccttcatg cagtcccggc cgccgcagca ctcgaccgga
     4321 taccagctgt gggccatggc gacgctcgct gtactggccg ctaaagccaa ggcgagccct
     4381 cccgaccgca gaatttggat caccagcatc cgtcgttgct ccatcggcct cgatttgcac
     4441 cggccttaac cagccgccat tgcaagggtt ccggaagcaa ggaccccggc ggggacccaa
     4501 aatgccgcat ttcccgtgct caagccgccc ttacgcggag tgccgtcgac aggagccagg
     4561 acgtgacgac ggggcccgtc ggaaactttg acccccatat cgacccttta gttgtgtgga
     4621 taagcgttgc attcgcgcta tagttgaaat cattttgtgg cggtttcgcc acagaattga
     4681 acattgttaa tttcggatgt tctgtggaaa aatagttcag cagacgtccg acagtttcaa
     4741 ttttgacaat tctgtggcgc ctgtctgctg acggcaaagc tgcttaggcg ccgatgtttc
     4801 cgttttgggc caaatttggg caaatcgcta cgtttctgcc ttcagatgca cgaccgcatt
     4861 gacgcgggta ttaaccaatc gaaaaaaaca gtcggacgtc gttgcccact tgcaccagcg
     4921 ctgcggagcg ctagatcgtc ccgtggtcaa cttcttagga gggcggcgga acttcggcga
     4981 ttaccgccgg agtgagggcg agagcaacag ggacggagcg gggccggacc tgttgctcgt
     5041 cccggcaaat gctccggttt tcatgcagtt cacgcgcaat cgtgcgtcgg tgttgtaact
     5101 catcccttcc gctgaccgtc gtaccgtgct aggtctgccc cgccaaaaac gtgacctcat
     5161 gaccagaaat tcgtggttgc aggcccgcac cactcgagtc tagggcgatc atacggcatg
     5221 aagtccgccg cgacagatct ggagactttc cttcagtcca ctctcggaac tcaagccagg
     5281 agaggcgcgc gcgtcctcag cggcccgacg atagcgctcg atgtgcgcgc aaccgccgta
     5341 ttgaagccgg tgctgcaaga gattgcctcg gacatcgcga cacatggctg tccggaccgg
     5401 ggtggggacg tcgcggtccg ctggtcttgt gatgatcagg gccgttgcgt catcgagctg
     5461 agcaacggga acatcgcgag cgcgaaaaga gcagacggcc gtgaggcgcc ggcaggagcg
     5521 gctcccacgg cgttcgaccg gccgggggat ggcatgatca gctgcgaaac gtcagagcag
     5581 ggtgctcgca tcatcgtccc cgcgcgatac gtttccgact ggaagcagcc cgatccgcgt
     5641 tccgtacctc cggccgtggc tcccgccgat ccgctcgaag gccgctcggt cctggtagtg
     5701 gaggatcagc tcatcattgc gctcgatctg gagatgctgc tcaaggagcg cggagccgtc
     5761 gcggtgcagc tcagcgggtc tgtgaacgac gctctcaaat cgatcgaagc agggcgtccg
     5821 gacatcgcca ttctcgacgt gaatctcgga agtacgacat ctttccccgt agcatgtgag
     5881 ctgcagcggc tgggcattcc
//
LOCUS       DASVDU010000221         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_86966, whole genome shotgun sequence.
ACCESSION   DASVDU010000221 DASVDU010000000
VERSION     DASVDU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86966"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VF226_14130"
     CDS             complement(<1..976)
                     /locus_tag="VF226_14130"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7075174.1"
                     /translation="MSGPVMADHSPPQGPQQAVSQAQATGDPPRQETRESAERRQPHH
                     HFRQAYAAIDLGTNNCRLLIARPASGNFVVIDAFSRVVRLGEGLAQTGRLSDAAMDRA
                     LGALKVCADKLRRRNVHLARSVATEACRRAANGAEFIERVRRETGIMLDIISAQEEAR
                     LAVLGCHILLEDGIGPAMIFDIGGGSTELVLIEPGGPVPRILDWQSVPWGVVSLSETV
                     GHEIEDADENARAARYAKMRQVVDDSFAEFAGRIAPHRAPDLRLLGTSGTVTTLASVH
                     LELPQYDRSAVDGLIVPSTSMREISARLSGMAPAERRTQPCIGNERADL"
     gene            1105..1178
                     /locus_tag="VF226_14135"
     tRNA            1105..1178
                     /locus_tag="VF226_14135"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1137..1139,aa:Gln,seq:ctg)
     gene            complement(1984..2928)
                     /locus_tag="VF226_14140"
     CDS             complement(1984..2928)
                     /locus_tag="VF226_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7075175.1"
                     /translation="MVVRQRLTRAKLLPFFARLPRCLVGMETCASANYWARELVALGH
                     EVRLMPPQYVKPYVKRSKNDASDAEAICEAVTRPTMRFAGVKSPEQQSVLVLHRTRSI
                     LTRQRTQLGNAIRAHLAEFGIVAPVGRQGLDRLMAIIGDESDDRLPQAARACLTMLVD
                     QYRLVLAHLLETDRAIRADSRATELGRRLQEIPGVGPLVASALVATVPDPQMFKSGRS
                     LSAWLGLVPRQHSSGGKERLGGISKAGDTYLRIMLFVGTMAAIRHAERHGTRRPWLMK
                     LLSRRHPRVAAIALANKIGRIAWAMMTSGERYREPVAA"
     gene            complement(2935..3069)
                     /locus_tag="VF226_14145"
     CDS             complement(2935..3069)
                     /locus_tag="VF226_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075176.1"
                     /translation="MSWTALHPAGIFAGQCGEEDTMEDVSVIGIDLAKSVFQVHGVDA
                     "
     gene            3127..3813
                     /locus_tag="VF226_14150"
     CDS             3127..3813
                     /locus_tag="VF226_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075177.1"
                     /translation="MHFHLPKPLHGWREFIGEVGIIVIGVLIALSAEQTVEWLHWRSV
                     VEDTRQALDGELAVDLGIIQARVEQAPCISRRLAELTTVFRRQADRQPLALKGPLGQP
                     QFPQLDTNVWDSVIADGATAHMPLDVRLRYSRFYSAIFWFRDKTDEESEAWSHLSQFD
                     DHDVLTQEDWSALHQWKARAQSLAAKVDANVSLATVRQFALSLGVEPRQFPFKSDSLA
                     ARDALCHPIT"
     gene            complement(4104..4541)
                     /locus_tag="VF226_14155"
                     /pseudo
     CDS             complement(4104..4541)
                     /locus_tag="VF226_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028714619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          783 a         1548 c         1465 g          885 t
ORIGIN      
        1 ccagatcggc ccgctcgttg ccgatgcagg gctgcgtgcg ccgctcggcc ggagccatgc
       61 cggagaggcg ggcggatatc tcgcgcatgg aggtcgaggg cacgattagc ccgtcgaccg
      121 cgctgcggtc gtactgcggc agctcgaggt ggacgctggc gagggtggtg acggtgccgc
      181 tggtgccgag cagccgcaag tccggcgcgc ggtgcggcgc gatccgtcct gcgaactcgg
      241 cgaagctgtc atcgaccacc tggcgcatct tcgcatagcg ggcggcgcgg gcgttttcat
      301 cggcatcttc gatttcgtgt cccacggtct cgctgagcga aaccaccccc cagggcaccg
      361 actgccagtc gagaatgcgc ggaaccggcc cgccgggctc gatcagcacc agctcggtcg
      421 agcccccgcc gatgtcgaag atcatcgccg ggccgatgcc gtcctcgagc aggatgtggc
      481 agccgagcac ggcgaggcgg gcctcctcct gcgcggagat aatgtcgagc atgatgccgg
      541 tttcgcgccg cacacgctcg atgaattccg ccccgttggc ggctcgccgg caggcctctg
      601 tggcgaccga gcgggcgagg tggacgtttc ggcgcctcag cttgtcggcg cagactttca
      661 gcgctccgag agcgcggtcc atcgcggcgt cgctcagccg gccggtctgc gccagccctt
      721 cgccgagtcg cacgacccgg ctgaaggcat cgatgacgac gaaattcccg ctcgccggcc
      781 gggcgatcag caaccggcag ttattggtgc cgagatcgat ggctgcatag gcttggcgga
      841 aatggtggtg cggctgacgc cgctcggccg attcacgtgt ctcttgccgc ggcggatcgc
      901 ccgtcgcttg agcctgcgag accgcctgtt gcggtccctg cggcggtgag tgatccgcca
      961 ttacgggtcc agacattcca acttccgccc gcacaaatcg cggggacctg cggcctaggt
     1021 agcgatgcta tcgggatcgg gcaagctgcg cccccgccag cgggttgacg gcgtgggtcg
     1081 ccctgcctag atgcgccccc tcgttgcccc gtcgtctaat ggtaagacta cggactctga
     1141 ctccgtcaat cgtggttcga atccacgcgg ggcatccagc tttctacaga taaatcatga
     1201 ttttagttcg agaatggctc gccctcgggc tttggggcca ttcccgtcga ttcccgccgg
     1261 atttcagcgg tttctcataa ttctcggcgg ctccgtgcaa catggatgca acacgatgca
     1321 tggatcgggt tcctggcggt tgatcggcgc aagccaaatg cggtcaaagc tggtctgaac
     1381 taggaggatg tcagctctaa tgcgaagcgg tcatctttcg ccgtttcccg cggacctccg
     1441 ggtgctccgg cagccccatt ccaagtgcaa gcccaaacca gttccaagcg ggttttacgt
     1501 tgtcgcggcg tcagcggagc ggccctgcgc caaccaatct cttcgtgagc acggcgagga
     1561 tggcccgtga tcgccggtat gaggacgcgc gaacctacca ggcgatcaaa cggggtcaga
     1621 ccggagctgc ggcacccagc cggacctagc acgccgaagc acgtccgcaa cctcgctcgt
     1681 tcacgcttag ccgaccagcc acaggcgacc ctgtctggac ggcgctccgt tagcaagatt
     1741 tggattatag cgttgcagct gcggttttgg gatgcggcca tgtcttcgac ctgttgatgc
     1801 ggcgttgcca tgtgccgctg gccatgatgc cttgtccgcc gatccggtag gtcccaacca
     1861 attgcacgcg cttggtagcg ccgggtcgtt aatgggtttt cctggggatc atcggtcccg
     1921 tcatcgggca gcttgcgtca gccagtctcc tgctcttgcc aacctcttga agttcgttcc
     1981 tcgttatgca gccaccggtt cgcggtaacg ctcgccgctg gtcatcatcg cccaggcgat
     2041 ccggccgatc ttgttggcca gcgcgatcgc tgccacccgt ggatggcggc ggctcagcag
     2101 cttcatcagc caaggccggc gggtgccatg ccgctcggcg tgacggatgg ccgccatcgt
     2161 gccgacgaac agcattattc gcaagtaagt gtcgcctgcc ttgctgatcc cgccgaggcg
     2221 ctccttgccg cccgacgagt gttgtcgcgg gaccagcccc agccaggccg acaagctgcg
     2281 ccccgacttg aacatctgcg gatcgggcac ggtcgccacc agcgcgcttg ctaccagcgg
     2341 cccgacaccg gggatctcct gcaagcggcg gccgagttcg gtcgcacggc tatcggcgcg
     2401 gatcgcacgg tcggtctcga gcaagtgcgc gagcaccagc cgatattggt cgaccaacat
     2461 agtcaggcag gcacgcgcag cctgcggtag ccggtcatcg ctctcgtcgc cgatgatggc
     2521 catcagccgg tccaagcctt ggcggccgac aggcgcaacg atcccgaact cggcgagatg
     2581 agcccggatc gcatttccaa gttgggttcg ctggcgggtg aggatcgatc gcgtgcgatg
     2641 gagcaccagc acgctctgct gctccggcga cttcaccccg gcgaaccgca tggtcggacg
     2701 ggtgaccgcc tcgcagatcg cctccgcatc cgaagcgtcg ttcttcgacc gcttcacgta
     2761 aggcttcacg tattgcggcg gcatcagccg cacctcgtgg cccagagcga ccagttctcg
     2821 cgcccagtag ttggcacttg cgcaggtctc catgccgacg aggcagcgcg gcagtctggc
     2881 aaagaacggc agcagctttg ctcgggtcaa tcgctgccgc accaccactt cgcctcacgc
     2941 atcgaccccg tgaacctgaa acaccgactt cgccagatcg atgccgatta ctgaaacatc
     3001 ctccatcgtg tcctcctctc cacactgacc ggcaaaaatg ccggccgggt ggagcgccgt
     3061 ccacgacatc aattgcggac ataagcgctt ctgttagctt gcactcgtta ccggggggca
     3121 agctgaatgc attttcacct gccaaagccg ctgcatggct ggcgcgagtt catcggcgag
     3181 gtggggatca tcgtcatagg cgtgctgatc gcgctcagtg ccgagcagac ggtcgagtgg
     3241 ctgcactggc gctcggtggt ggaggacacc cggcaggcgc tcgatgggga actggccgtc
     3301 gatctcggga tcatccaagc acgcgtcgag caggctccgt gcatttctcg tcggcttgcg
     3361 gagctcacga ccgttttccg gcgccaagcg gaccggcagc cgctggcgct caaaggtccg
     3421 ctggggcagc cccaatttcc gcagctggac accaacgtgt gggactcggt catagcggac
     3481 ggggcaacag cgcacatgcc gctcgacgtc cggcttcgct attcccgttt ctactcggca
     3541 atcttctggt tccgcgacaa gacggatgag gaatccgaag cctggagcca cttgagccaa
     3601 ttcgacgacc acgacgtgtt gacgcaggag gattggtctg cgcttcatca atggaaggcg
     3661 cgcgcccagt cgctcgctgc gaaggtggat gcaaatgtgt cgctggcgac cgtgcgccag
     3721 tttgccttgt ctctgggcgt cgaaccgcgc caatttccgt tcaagtccga ctctttggcc
     3781 gcgcgggacg ccttgtgcca cccgattaca tgaggccgcc gaccaagtga ccgctttcca
     3841 tctgtggacg ccccccggag cgcaagcgat ttttgggttt gtctgacacc tggtcggatg
     3901 ctgccatctg tccggcttct gttgcggcga tgatgagtgc cgcgagcccg tatgggagtt
     3961 cgcggatcgg atccaaatca cccactagcg tgcttgggtg gcacggtgga aagcactggt
     4021 tcttctgatc ccgtctcgcc gactgtggtg ccatactctc cttcgatcgc taacgctctg
     4081 gcatcctcac gcccgacgca gctctaggcc gcgacagccg gagctcgata aactcctcca
     4141 ttggcgagca ccgcccagac gatccgcgcg gtcttgttgg cgagcgcaac ggtcaccagc
     4201 atgcgcggct ttctcgccat catctgctcg agccacgatc ccttgggtgc gccgcgcctg
     4261 ctcgcgtgaa gaacgacggc agcgctgccc aggatcagca agcgcctcaa cgttcgctcg
     4321 cccatcctgc tgatcgaacc aagcctcgcc ttgccaccgg tcgatcgctg cagcggtgtg
     4381 aggccaaccc aggcggcaaa gtcgcagccc ttgcgaaagc tctccggagg tggggccaag
     4441 gcaaccagcg cggtggccgt gatcggaccg attcccggaa tggtcatcag ccgacgcgcg
     4501 gtctcatcct cgcgggctcg cttcgtgatc tcgcggtcga gcaggccaat gcgttcgtcc
     4561 aatgcctttg cgctcacacc atcaccagga acacatcacg cgcactcggc ggcaacgcgc
     4621 acccaggatc ctcgatcttc gccatcatcg cgccggcatg gaacagcccc ttgggcgcga
     4681 c
//
LOCUS       DASVDU010000222         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1396314, whole genome shotgun sequence.
ACCESSION   DASVDU010000222 DASVDU010000000
VERSION     DASVDU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396314"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF226_14160"
     CDS             complement(<1..921)
                     /locus_tag="VF226_14160"
                     /inference="COORDINATES: protein motif:HMM:NF027404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7075178.1"
                     /translation="MSSLEEQYLSTPLSGANAAAVERLYEEFVADPRSVPESWRRYFE
                     SLGGRGDEVLHSEVRREVEQAVRHRRANGQAGAAVAAPHSAASEKQAAVSRLIQVYGL
                     RGHQIADIDPLGLMERPTPGVLKLDWLGLGEADMETEFFTGGLAGTGHRRMQLKDILA
                     LLKSIYCGKIGAEFAHVSRSRERLWLREAFEKGMVSRHFAPEEQLWILEQLTAAEGIE
                     RYLHTRFVGQKRFSLEGGESLIPMLDTLIQSGGESGIREFVIGMAHRGRINVLVNVLG
                     KPPEELFDEFEGNIDPEQLKGSGDVKYHKGF"
     gene            complement(1071..1607)
                     /locus_tag="VF226_14165"
     CDS             complement(1071..1607)
                     /locus_tag="VF226_14165"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HEX7075179.1"
                     /translation="MRDGDRDGAGSSRMAMAQAMSEITSMEDAGTFSDTRVLPDRRRF
                     TWRTVVFGYLRSRRRRHRRDSERDAPFIDWHHPWLFFLAVGIMLLSTLDAFLTLRLLE
                     RGAIEVNPVMAAMLGHGHFTFAASKMALTGLGIMTLVFLSRLRVFNLMRTGVLLTLFF
                     SCYACLACYQLLMLLGSL"
     gene            complement(1600..2142)
                     /locus_tag="VF226_14170"
     CDS             complement(1600..2142)
                     /locus_tag="VF226_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075180.1"
                     /translation="MSPLRALLPTVVAVTAVACSRSGPERAAATPEEQPGYLTAATLG
                     EQVVLPTADYLKLARYREADVEYGRNLALQCRTCHNLDDSASSPLGPSLYGLFGRRAG
                     SLGDFSYSPAMQAADFVWTPRALDAWIAAPWRFVPGNRMSFVGLTDQDARDALIAALL
                     QITEGGTDKDGQDEESEGGA"
     gene            2346..3206
                     /locus_tag="VF226_14175"
     CDS             2346..3206
                     /locus_tag="VF226_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX7075181.1"
                     /translation="MSAPQTFRVSIVAVMLFQIAALFAKSRLELELVDTGFSASVAKH
                     LSYLVVPPILLILMYPYIARCKSALRSLLQLKDLTAQRVLRAGLVGLTLRLLRWAFLT
                     VLIWLGVTQDSEGGMFPMHLGFACPPLPVLLLSVLVMTVLVPFVEEVVNRGFIMHALL
                     ARGRALAVILSAALFAALHPPGSYALTFAAGLVFAVQALNFRTLWAPLSAHLAYNAAA
                     IVDWECLKIVWNPSPGDPLLARLALLSAAALIVGSWLVWRLTRERAAGGSVPVSSPAV
                     PTTGRSPAAR"
     gene            complement(3178..3369)
                     /locus_tag="VF226_14180"
     CDS             complement(3178..3369)
                     /locus_tag="VF226_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075182.1"
                     /translation="MRELALHELTQVAGGGDLSCGPDDGNNYGGVSDTESFGDDVINL
                     YEGLVSATSYIIERLANAL"
     gene            3584..>3816
                     /locus_tag="VF226_14185"
     CDS             3584..>3816
                     /locus_tag="VF226_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075183.1"
                     /translation="MHALRTCLLLAGLAAAANVEADDNLWLGVKAGTLGLGVEGTWRP
                     LPWFDLRAGLNRFEYDDDGTEAGIDYDATLDLGT"
BASE COUNT          623 a         1250 c         1257 g          686 t
ORIGIN      
        1 gaagcccttg tggtatttca cgtcgccgga gcccttgagc tgctccggat cgatgttgcc
       61 ttcgaattcg tcgaagagct cctcgggtgg cttgccgagg acgttgacca gcacgttgat
      121 gcggccgcga tgggccatgc cgatcacgaa ttcgcggatt cccgactcgc cgccgctctg
      181 gatcagcgtg tcgagcatcg gaatgaggct ctcgccgcct tcgagcgaga accgcttctg
      241 gccgacgaag cgcgtgtgca ggtaccgctc gatgccttcg gccgccgtga gctgctccag
      301 gatccagagc tgctcctccg gtgcgaagtg ccgggacacc atgcctttct cgaacgcctc
      361 gcgcagccac aggcgctcgc gcgaacgcga gacgtgcgcg aactcggcgc cgatcttgcc
      421 gcaataaatg cttttcagca gcgcaaggat gtccttgagc tgcatgcgcc ggtggccggt
      481 gccggccagc ccgcccgtaa agaactccgt ctccatgtcg gcttcgccga ggcccagcca
      541 gtcgagcttg agaacgccgg gtgtcggcct ttccatgagc ccgaggggat cgatatcggc
      601 gatctggtgg ccgcgcaggc cgtagacctg gatcaggcgc gaaacggcgg cctgtttctc
      661 gctcgcggcg gagtgcggcg cggcgacggc cgcgcccgcc tgcccgttcg cgcggcggtg
      721 acggacggcc tgctcgacct cgcggcgtac ctccgagtgc agcacctcgt cgccgcggcc
      781 gccgagcgac tcgaaatagc ggcgccagct ctccggcacg gatcggggat ctgcgacgaa
      841 ctcctcatat aggcgttcga cggcggctgc gttggcgccg gacagcggcg tgctcaggta
      901 ctgttcctcg agggaactca tggcgcagaa cggttcacga aaccagctgt ttcgagagtc
      961 tcggcagtgg gaaacggggc cggtagttta gcccaatgtc agggagcaac actagacgcg
     1021 caaaccgctg cgtcgacacg ctcgcggccg cgcggtcttc gagctccggc tcacaaggag
     1081 ccgagcagca tcaggagctg gtagcaggcg aggcaggcgt agcagctgaa gaaaagagtg
     1141 agcagcacgc ccgttcgcat caggttgaag acccgcagac gcgaaaggaa gacgagggtc
     1201 atgatcccga gtcccgtcag ggccattttg ctcgccgcga aggtgaagtg accgtgcccg
     1261 agcatcgcgg ccatcacggg gttcacctcg atcgcgccgc gctcgagcaa ccggagcgtc
     1321 aaaaaggcgt cgagggtcga gagcagcatg atgccgacgg cgagaaaaaa gagccacggg
     1381 tggtgccagt cgatgaacgg ggcatcgcgt tccgaatcgc ggcggtgccg gcggcgccgg
     1441 gatcgcaggt agccgaaaac cacggtgcgc caggtgaagc gtcgccggtc cggcagcacg
     1501 cgcgtatcgg agaatgtccc ggcatcttcc attgacgtga tctcgctcat cgcttgtgcc
     1561 atcgccatcc ggctgctgcc ggcaccgtcc ctgtccccgt cacgcaccgc cctccgactc
     1621 ctcgtcttgt ccgtccttgt ctgttccgcc ttccgtgatc tgcagcagag ccgcgatcag
     1681 cgcgtcgcgt gcgtcctggt cggtgagtcc gacgaacgac atgcgattgc ccggtacgaa
     1741 acgccagggc gcggcaatcc aggcatccag cgcacgcggc gtccacacga aatccgcagc
     1801 ctgcatcgcg ggtgagtagg aaaaatcgcc gagtgagccg gcgcggcgcc cgaacagccc
     1861 gtaaaggctg ggaccgagcg gagacgaggc actgtcatcc agattgtggc aggtgcgaca
     1921 ctggagcgcg aggttgcggc cgtactcgac gtccgcttcg cgataacgtg cgagcttcag
     1981 gtaatcggcg gtcggcagca cgacctgctc gcccagtgtc gcggcggtca ggtaaccggg
     2041 ttgctcctcc ggcgttgccg ctgcccgctc gggtccggag cggctgcagg cgacggcggt
     2101 gaccgcgacg acggtcggca gcagcgcgcg caaggggctc atcgtgcagg tccctctgtt
     2161 cggatgacgg ccgcctcctg gccgccccgg gattgtctgc gaggttcgcc gatttggcaa
     2221 tgacgcggtt cacgcaggtt cggtccgcgc cggcgagccc aatttcgcgc caaaaacccg
     2281 aattatggtg aaggcacctg ctgccgcagc cgcggtaggt tgcgcctcct cgcaaggcgg
     2341 tacgcatgtc tgcaccacaa acattccggg tgtccatcgt tgcggtcatg cttttccaga
     2401 tcgccgcgct gttcgcgaag agccggctcg agctggagct cgtagatacc ggtttcagtg
     2461 catccgtggc gaagcatctc agctatctcg tcgtcccgcc catcctcctc attctcatgt
     2521 atccgtacat cgcccgatgc aaaagtgcgt tgcggagtct gctgcagctg aaggatctga
     2581 cggcgcaacg ggtactgagg gccggcctgg tcggcctgac gctgcggttg ctgcggtggg
     2641 cctttttgac agtcctgata tggctcgggg tcacgcagga cagcgaggga gggatgttcc
     2701 cgatgcacct cggcttcgcg tgtccgccgc tgccggtcct gctgctcagc gttctcgtga
     2761 tgacggttct cgtgccgttc gtcgaagagg tcgtcaaccg cggattcata atgcatgcac
     2821 tgctggcacg tggaagagcc ctggcggtga tcctttcggc cgcgctcttc gcggcgctgc
     2881 atccaccagg cagctacgcc ctcaccttcg cggcgggcct cgtgttcgcg gttcaggcgt
     2941 tgaacttccg caccctgtgg gcgcccctgt cggcgcacct ggcgtacaac gccgccgcca
     3001 tcgtcgactg ggaatgcttg aagatagtgt ggaatccctc gccaggcgat ccgttgctgg
     3061 ccaggcttgc cctgctgtcg gcggcggccc tgatcgtcgg ctcctggctg gtgtggcggc
     3121 tcacacgtga aagggctgcc ggcgggagcg ttcccgtcag cagccccgcg gttccgacta
     3181 cagggcgttc gccagccgct cgatgatgta cgaggtggca ctgaccagcc cctcgtacag
     3241 attgatgacg tcgtcgccga agctctcggt atcgctgacg ccgccgtagt tgttgccgtc
     3301 gtccgggccg cagctcagat cgccgccgcc ggccacctgc gtcagctcgt ggagtgcgag
     3361 ttctctcatg acgttcaacc tccgtgttgt tggaatggat gcgcatcctg cgcacgctcg
     3421 aaagctacga cctgcggatg atcccgacga cccctgaaac tggcccgaat ggacggattt
     3481 gccccctgag cgaaaaccgc cgggcgcgtg atccgggtca cggccccgcg cccggcgccc
     3541 ggctaggctc tgcttttccg gcgtcgctca ctggaggacc gccatgcacg cactacgcac
     3601 ctgccttctg ctcgccggtc tcgcggccgc tgcgaacgtc gaggccgatg acaatctgtg
     3661 gctcggcgtc aaagccggca cgctcggcct cggtgtcgaa ggcacctggc ggccgctgcc
     3721 ctggttcgac ctgcgcgccg gcctgaacag gttcgaatac gacgacgacg gaaccgaggc
     3781 cggcatcgat tacgacgcga cgctcgacct gggtac
//
LOCUS       DASVDU010000223         5591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_610269, whole genome shotgun sequence.
ACCESSION   DASVDU010000223 DASVDU010000000
VERSION     DASVDU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610269"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VF226_14190"
     CDS             <1..935
                     /locus_tag="VF226_14190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075184.1"
                     /translation="GLAHAMRQQAKRGRIDVVQCHWLVPQAVVAALALRRNAPPFVIT
                     CHGSDLYGLNQRWLKALKRFALRRAAAVTTVSEPLKGEIRSLMGESEWPERTAVVSMG
                     VDTERFAPSRRNPDWAPSKALGRPVVLFVGRLVEGKGVSFLIQALGNPVLAQTTVCAA
                     IVGDGPQRQALEEQARYSGLGERIRFLGSVAHDALPEIYASCDIFCLPAIRTARGEEE
                     GFGLVLAEAASSGLPIVAARVRGAQGLLEDGVTGSMVAERDPVALAKALSALASDAGL
                     AARFGANARQQALKYDWQAIARRYRDLLACPCEM"
     gene            complement(959..2092)
                     /locus_tag="VF226_14195"
     CDS             complement(959..2092)
                     /locus_tag="VF226_14195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075185.1"
                     /translation="MRIAWITAAPISAIVGGGATYSRSVFRVFEGLPQTPRLIEVPLR
                     SRPMRLGHKLEQGMAFARASLSKLPAKAKFQMPCGAVRALRRKIAAIEPDIAVLGSAD
                     LLPCRAALDGVPFVLIAHNVEQQLYEDQVRAAMDRFRFGPAAWWLSRDLAKLRRMETE
                     GVRAANLIIAVSRNDADTLQELGARSPVFVLPPTFPGPLPDHPRPAVERPLKLALVAK
                     MTWWPNRKAVDWLAREVMTRLPSGTAELHLYGPGSERIADRKSGVFGHGFVPDLREVW
                     RAGHIAVCPVHQGGGVNVKVAEAIFNGSPILATPHGLRGLPAMISDPAVAVRGSSAEW
                     IDFLQSNDAEDLASRTPKAETRMLFADSAYVAPLGDALARSHS"
     gene            complement(2109..3218)
                     /locus_tag="VF226_14200"
     CDS             complement(2109..3218)
                     /locus_tag="VF226_14200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7075186.1"
                     /translation="MDRKPLASYSSAIGAPAPGTAIVVLNWNGWSDTIECLESLFRLD
                     PGPQRIVVCDNASEDNSLDHLIAWAEGRLNTIPPTKADETRRLSFPPVVKPIPYKVYE
                     RAEAEAGGDLPDDAPRLAFIRTGANLGFAGGNNVGLRYLLADSSISAIWILNNDTVVE
                     RSALGALVSEALSNPAAGLIGATVLEYERPSMIQAQAGAKYLRCIGWATHIGAKRKWS
                     DRLKKNSVEQQVDYILGASMFATRAFLEKAGLMDESYFLYYEELDWATKAAGRGALGY
                     APDAIVYHKMGRSIGTGRRLASGSALSLYYLNRNRLLIARRHWPRFLLTTAAVNLFMT
                     ALLLMRFRLREAKAVLRATAVALSKPLDVAFRSAF"
     gene            complement(3245..4105)
                     /locus_tag="VF226_14205"
     CDS             complement(3245..4105)
                     /locus_tag="VF226_14205"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX7075187.1"
                     /translation="MAVPTYPPVIIIGMHRSGTSLLTRMLNCLGLFIGARRDVNDEAI
                     FFQRENDWLLKQAGGTWDNPQSVGALLAHCDRRSACLRHLERRLRSPYLWRYLGMSQF
                     LRYRSAFSLDFAWGWKDPCNTYTLPLWLQLYPDARVVHIRRHGVDVAQSLVKRETGKE
                     AADSIASTLGSGLRRLNGYPSRVAPSLCCETLEGAFGLWEEYSAEARRHVGKLGPRAF
                     ELSYEQLLEAPQDQLRALCHFCGLHVEESAIGEVASMASRSRARAFRRDDVLAAFAQR
                     VRSRLAIYDY"
     gene            complement(4107..>5591)
                     /locus_tag="VF226_14210"
     CDS             complement(4107..>5591)
                     /locus_tag="VF226_14210"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX7075188.1"
                     /translation="GVSGGPGRPWYDFLSIFRKDIGVPHDPVSAVPKESGPVRAAERA
                     SIDPLTRRFDLFLRDGLSGAARAFGLRMLGIAAGFLANLLLAHALGAHGMGIVALAVT
                     VAGVPAALAVLGLNNALLRFAGAAAARQDWRTILGLARVSLRLSIVASVLGGATVFVA
                     APLLARDLFDEPALTLTLRLAALSVLPLAVNSVLAGLMKAIGRPAVATLAEMAGLSLA
                     WLIGLSLLALMLGPTLGPVSAIGAYSGGSLLVLALVFLIWRRSTPRVPAEVSEAETRQ
                     LLRSSIPLFWIASVHLVASWADTLVLGAFLDAEAVGIYNVALRLASLVSLTLIAVNTV
                     LPQRFAALFADGRTDELQRLARQVTAFLWIVSLPVVLLFVLWPSGPLRLFGPEFVDGR
                     FALVVLTLGHFVNVATGPVGYLLMMSGQERLLRNVVVVATIINIALDLLLIPGFGVNG
                     AAAASAASLALLNISSLALVYRKLGLSPMPWSALRRHGTPTIAR"
BASE COUNT         1166 a         1771 c         1749 g          905 t
ORIGIN      
        1 ttgggctggc gcacgccatg cggcagcagg cgaagcgcgg gcgaatcgac gtcgtacagt
       61 gccactggct cgttccgcag gcagtcgtcg cggcgctggc gcttcggcgg aatgcgcctc
      121 ccttcgtcat tacctgccac ggcagtgacc tctacgggct caatcaacgc tggctgaagg
      181 cactgaagcg attcgcgctt cgtcgggctg ccgccgtgac aacggtctcg gaaccgctga
      241 agggggaaat ccgctccctg atgggagaga gtgaatggcc ggaaaggacg gccgtcgtct
      301 cgatgggggt cgacaccgaa cggtttgccc cgtcaagaag gaatcccgac tgggcaccct
      361 cgaaggcgct agggcgtccg gtcgtccttt tcgtcggccg gcttgtcgaa ggaaagggcg
      421 tgtcttttct tattcaggcc cttgggaatc ccgttctggc gcaaacgacg gtctgtgcgg
      481 cgatcgtcgg agacggccct cagcgccaag cgctcgagga acaggcccgg tactccgggc
      541 tgggcgagcg gattcgcttc ctgggttcgg tggcgcacga tgcgctgccg gagatctatg
      601 cctcctgcga catcttctgc ctgccggcca tccggacggc gcgcggcgag gaagagggtt
      661 tcggcctcgt gctcgcggag gcggcaagca gcgggcttcc catcgtggcg gcaagggtgc
      721 gcggggctca gggactcctg gaagacggcg tgaccggctc catggttgcg gagcgcgacc
      781 ccgtcgcgct ggcgaaagcg ctttcggcac tggcctccga tgccggcctc gcagcccggt
      841 tcggggcaaa cgccagacag caagcgctca aatatgattg gcaagcgatc gccagaagat
      901 accgcgacct tctcgcttgt ccctgcgaga tgtaatcttg atgatttccg tcgagctttc
      961 aggaatgtga acgggccagg gcatcgccca gcggagcgac ataggcactg tcagcaaaca
     1021 acatccgcgt ttccgccttc ggtgtccggc tcgcgagatc ttcggcgtca ttggactgca
     1081 ggaagtctat ccactccgcc gagctgccgc gaacggcaac cgccggatca gagatcatgg
     1141 ccggtaagcc gcgcaatccg tgcggcgtcg ccaagatcgg cgacccgttg aagatcgcct
     1201 cggccacttt gacgttgacg ccgccgccct ggtgcacggg gcagacggca atgtgcccgg
     1261 cgcgccagac ctcccgaagg tccggaacga acccgtgtcc gaaaacgccg gacttgcgat
     1321 ccgcaatccg ttcggagccc ggtccataca gatggagttc cgctgtcccg gaaggcaagc
     1381 gtgtcatgac ctcccgcgcc agccaatcca cggcctttcg attcggccac catgtcatct
     1441 tcgcgaccag cgccaacttg agcgggcgct cgaccgctgg ccttgggtga tcaggcaaag
     1501 ggccgggaaa cgtgggcggc agcacgaaca caggcgatcg cgcgcccagt tcttggagcg
     1561 tgtcggcatc gttccgggaa acagctatga tcaagttcgc cgcccggaca ccctcggtct
     1621 ccatgcgccg cagcttcgcc aaatcgcgcg agagccacca agcggccggc ccgaacctga
     1681 accggtccat cgcggctctc acctgatctt cgtagagctg ttgctcaaca ttgtgcgcga
     1741 tcaggacgaa cggaacgccg tcgagcgccg cacggcacgg cagaagatcg gcggagccga
     1801 gaactgcaat gtcaggctca atcgccgcga tcttgcggcg cagtgccctg accgcgccgc
     1861 agggcatctg aaatttcgcc ttggccggca gcttggaaag ggaggcccgc gcaaaggcca
     1921 tgccttgttc cagcttgtgc ccaagccgca ttggacgaga ccggagcggc acctcgatca
     1981 gccggggcgt ctggggcaag ccttcgaaca cccggaatac gctacggctg taggtcgcgc
     2041 caccgccgac gatggcggat atcggtgctg ccgtgatcca ggcgatgcgc atctaccggg
     2101 aaatcgagtc agaacgcgga acgaaaggct acatcgagcg gcttactcaa ggcgacagcg
     2161 gtcgcgcgca agacggcttt cgcctctcga aggcgaaatc gcatgagcag aagggcggtc
     2221 atgaacagat tgacggcggc ggtcgtgaga aggaagcggg gccaatggcg gcgcgcgatc
     2281 agcaacctgt tcctgttcag ataatacaag gacaaagcac tgcccgaggc caagcgccgg
     2341 cccgttccga tggagcgtcc catcttgtga tacacgatcg catcgggtgc atatcccaaa
     2401 gcaccgcggc cggccgcctt tgtcgcccaa tcgagttcct cgtaataaag aaagtagctt
     2461 tcgtccatga ggccggcttt ctcgagaaag gccctggtgg caaacatcga cgcacccaga
     2521 atgtagtcga cctgttgttc gacggaattc ttcttcaagc ggtcggacca tttccttttg
     2581 gctccgatat gcgtcgccca tccgatgcac cgcagatatt ttgcccccgc ctgcgcttga
     2641 atcatgctcg gccgctcgta ttccaaaacg gtcgcaccga tgaggcctgc cgcgggattc
     2701 gatagagctt cggaaacgag ggcgccgaga gcgctccttt cgaccacggt gtcgttgttc
     2761 aggatccaga tggcggatat cgaggaatcc gcaagaagat atcggagacc gacattgttt
     2821 ccgccagcga atccgagatt ggcaccggta cggatgaaag cgagtctcgg agcatcatcc
     2881 ggcaaatcgc cgccggcttc agcctcggcg cgctcatata ctttataggg gatcggcttc
     2941 accacaggag gaaaggagag acgccttgtt tcgtctgctt tcgtcggagg gatagtgttc
     3001 agccggcctt cagcccaggc gatcagatga tcgagagagt tgtcctcgga agcattgtca
     3061 cacaccacga tccgctgcgg accgggatca aggcggaata gactctccaa gcactcgatc
     3121 gtatcggacc agccgttcca gttcaaaacg acgatcgctg tgccgggagc tggtgcgcct
     3181 attgccgagc tgtagctcgc aagtggcttt cgatccattg ccggcaccat ctcacatgcg
     3241 caggttaata gtcgtagatc gcgagcctgc tgcgcactct ttgagcaaac gcggcaagga
     3301 catcgtcacg acggaaagcc cgtgctcgtg accgagaagc catactggcc acctcgccga
     3361 tagcgctttc ttccacatga aggccgcaga aatggcaaag cgcccgtagc tgatcctgcg
     3421 gcgcctcgag gagctgttcg taggagagtt cgaaagcccg agggcccagc ttgccaacgt
     3481 ggcgccgagc ttcggccgag tactcctccc aaaggccaaa ggcaccttca agcgtctcgc
     3541 aacataggga aggcgccaca cgactaggat acccgttcag gcggcgcaga ccgcttccca
     3601 acgtagacgc tatcgaatcc gccgcctctt ttcccgtttc tctctttacg aggctctgcg
     3661 caacatcgac cccgtgccgg cgtatgtgca caaccctcgc atccggatac aactgcagcc
     3721 aaagtggaag cgtgtaggta ttgcagggat ccttccagcc ccacgcaaaa tcaagactga
     3781 acgcggagcg gtacctcagg aattgggaca tgccgagata gcgccagaga tacggagaac
     3841 gcagccgccg ctccagatgc cgaaggcagg cactacgccg gtcacaatgc gccaaaaggg
     3901 ctcccaccga ttgcggatta tcccaggttc cgccagcctg cttcagcaac caatcgttct
     3961 cccgttggaa gaagattgct tcgtcattca cgtccctccg cgcgccgatg aacaggccaa
     4021 ggcagttcag catgcgcgtc agcagactcg tcccggaacg gtgcataccg attatgatga
     4081 ctggagggta cgtcggcacg gccatatcag cgggcaattg tcggggttcc gtgccgtcga
     4141 agtgctgacc acggcatcgg cgaaagaccc agcttacgat acaccaaggc gagcgaggag
     4201 atattgagca gcgcaaggct tgccgcgctt gccgccgccg cgccattgac cccgaacccc
     4261 ggaatgagca gcagatcgag ggcgatgttg atgatggtcg cgacgaccac gacgttcctg
     4321 agcagccgct cctggccgct catcatgagg agatagccga ccggaccggt cgcgacattc
     4381 acgaaatggc ccaaggtcag gacaacaagc gcgaagcggc catcgacgaa ctccggcccg
     4441 aacagccgca acggacccga tggccacagg acgaagagca gaacgacggg cagcgagaca
     4501 atccacagga acgccgtcac ctgccgggcc agcctttgca gctcatccgt tcgcccgtcc
     4561 gcgaagaggg ccgcgaagcg ctggggaagg acggtattga ccgcgataag cgtaaggctg
     4621 acgaggctgg cgagccgcaa ggcgacgttg tagatgccga ccgcctccgc atcgagaaac
     4681 gcacccagca ccagcgtatc cgcccagctc gccacaagat gcacggaggc gatccaaaat
     4741 agcgggatgc tgctcctcag aagctgccgg gtctccgcct cgctgacttc cgccggaacc
     4801 cgaggcgtgg accgccgcca gatcaggaac accagagcaa ggaccagaag ggagccgccc
     4861 gagtaggcac cgatcgcaga gaccgggccg agcgtcgggc caagcatcaa ggcaagcagg
     4921 ctcaagccga tcaaccaggc gagggaaagc cccgccattt ccgcgagcgt tgcgaccgcc
     4981 ggcctgccga tggccttcat cagtccagcc agcacgctgt tgacggcgag cggaagaaca
     5041 gaaagcgcgg ccagtcttag ggtcagggtg agcgcgggct catcgaacag gtcgcgggcc
     5101 agcaacgggg ccgcaacgaa gactgtcgca ccgcccagca ccgatgcgac gatcgagagc
     5161 ctgagcgaga cacgggcgag gcccagaatc gtccgccagt cctgccgcgc cgccgcggca
     5221 ccggcgaagc gcaggagagc gttattcagc ccgaggaccg ccagcgccgc cggaaccccg
     5281 gcaacagtca ccgccagggc gacgatgccc atcccgtggg cgcccagggc gtgagcgaga
     5341 agcaaattcg ccaggaatcc ggctgcgatc ccaagcatgc gcagcccaaa ggctcgggcc
     5401 gcgccggaca ggccgtcgcg caggaaaagg tcgaaccggc gggtcagggg atctatcgag
     5461 gctcgctcgg cagcgcgcac ggggccggac tccttcggga cggcgctgac aggatcatgc
     5521 ggaacgccga tatccttgcg gaaaatgctt aagaagtcgt accacggccg ccccgggccg
     5581 cccgagaccc c
//
LOCUS       DASVDU010000224        16936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_91700, whole genome shotgun sequence.
ACCESSION   DASVDU010000224 DASVDU010000000
VERSION     DASVDU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16936
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91700"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /gene="holA"
                     /locus_tag="VF226_14215"
     CDS             complement(<1..1038)
                     /gene="holA"
                     /locus_tag="VF226_14215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7075189.1"
                     /translation="MTAIKTFQADRFLKTFDSRYRAILVHGPDGGAVAERARAAAQRL
                     AALSDPPGEILRIEDADLEQDPDRLLVETQTIPMFGGPKVVHTRASRRVNAASLKPLL
                     EGGALAGSLVVEAGNLRSNDALRLLFEKSPHAAAVICYPDEARDLEAIVRDTLQEAGV
                     AITPDARQTLLSRLGADRAMTRGELEKLILYARGKSQIDVDDVEAVVGDASELAIDKV
                     LFAVASGEANRALAEYDRVITSGSGPQSVIAALQWHFQRLHKLRTAIDAGGSLEQILR
                     QLRPSPPQKVQDALQAQCRMWTGGKLITALSRIAETLKAARLNDALESPMTERLILDL
                     ARIASDRPPARS"
     gene            complement(1096..1659)
                     /gene="lptE"
                     /locus_tag="VF226_14220"
     CDS             complement(1096..1659)
                     /gene="lptE"
                     /locus_tag="VF226_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX7075190.1"
                     /translation="MSSPRGQEDSTPSWERRSFLRAVAFAGLLGPVLAACEGASAFRP
                     MYGASNMADTEAKLASVSFGTIPGRVGQRIRNELVFQSTGGGHGLPPKYHLDVAIREQ
                     VVSTLVRKDGESQSQVYNLEAKFQLIRIADKAVLLEGTSYGRAGFERYDSIYSNVKAR
                     EDAENRAARTVAQDIKTRLAAYLAGTA"
     gene            complement(1646..4243)
                     /gene="leuS"
                     /locus_tag="VF226_14225"
     CDS             complement(1646..4243)
                     /gene="leuS"
                     /locus_tag="VF226_14225"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7075191.1"
                     /translation="MAIERYNAPAREKHWQHVWESRNVFQASNEHTRPKCYVLEMFPY
                     PSGRIHMGHVRNYTMGDVLARYKRALGFNVLHPMGWDAFGMPAENAAMERKTHPAKWT
                     YDNIAAMRAQLKSMGLSLDWSREFATCDVEYYHQQQKLFLDFYKEGLAYRGVGKVNWD
                     PVDQTVLANEQVIDGRGWRSGALVETREMPVWYFRITKYADELLDALKDLTRWPEKVR
                     VMQANWIGRSEGLLMKYELSGERLPAGWRELEVFTTRADTIFGGSFCALSPGHPLTLE
                     LAKDNPELAGFLEDCRHVGTSEEDLNRVEKKGFNTGLTATHPLIPDRKLPVFVANFVL
                     MGYGTGAIFGCPAHDQRDFEFATAYKLPIVPVVIPEGQDPATFDLGGEAYDGPGRIAN
                     SGFLNGLTIDEAKAEVARRMEEIGKGERRTMYRLRDWGISRQRYWGCPIPIIHCDACG
                     IVPVPEDQLPVKLPEDVTFDVPGNPLVRHPTWKHVDCPSCGKPATRETDTMDTFVDSS
                     WYFARFCSPHADVPVDLDAAKYWLPVDQYIGGIEHAILHLLYSRFFARAMVATGHLNV
                     KEPFGGLFTQGMVIHETYSNRNGQWLLPSEVRIEGEGDQRRAYEIATGAPVQIGPVEK
                     MSKSKKNLVDPDDIISTYGADCARWFMLSDSPPERDVIWTEAGVSGASRFMQRVWRLI
                     DEALPKAAPKGSPKPGEFGPDADALRRAAHKALAKVSQNIETLRFNVAVAQIYEFAST
                     LHAALGKDGPGMAWAIREAAEILVQMFGPMMPHLAEECWERLGYNTLLADQPWPTVEE
                     ALLVDDTITIAVQVNGRRRDELTIARNASKAEVEAAALKLEPVIRALEGRPVKKVIVV
                     PQRIVNVVA"
     gene            4466..5128
                     /locus_tag="VF226_14230"
     CDS             4466..5128
                     /locus_tag="VF226_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7075192.1"
                     /translation="MSAAAERLMNIRERITAAARDAGREPSSVRLIAVSKTFDAPHVL
                     PVIEAGQRLFGENRVQEARAKWPPLKERFPDLELHLVGSLQSNKAKEAVQLFDAIHSI
                     DRPKIARAVADEMAKQGRRLKLFMQVNTGEEPQKAGVMPREAAALIRHCREEMKLDIA
                     GLMCIPPADEEPAIHFAFLAKLASEFGLKELSMGMSADFETAISFGATYVRVGSAIFG
                     AR"
     gene            5271..6533
                     /locus_tag="VF226_14235"
     CDS             5271..6533
                     /locus_tag="VF226_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7075193.1"
                     /translation="MRALSVFAAAGAALIALPAVAQQPSAGNLEKLSKFQSTGTPIDI
                     PQIPQQGPKADQIRKNLKNIKLPPGFKIDLYALVPDARHMAVGPQGVVTFVGTRKNKV
                     YAVTDRAKMGVAEEVKEFAPSIQFKIPNGVCFSPDGFLFIAEQNRVLVFPAAEFFYES
                     PDVAAFVVVKQGELIPPEEESFNHTARVCRIGPDDKLYITLGQPFNVFEKEKMDLYKR
                     TGMSGIIRMNREGRQREVFATGVRNSVGMDFNPKDKSLWFTDNQVDGMGDEIPPGELN
                     RADRAGLHFGFPWYGGGKVRTVEYKDSEPPANVVFPQVEMDAHAADLGMTFYTGKQFP
                     EKYRGGIFSAQHGSWNRTNPVGARVMFTSLKPDGTADKTEVFAEGWLTPNAEYMGRPV
                     DVAQLRDGSILVSDDFAGALYRISYEGN"
     gene            6520..6849
                     /locus_tag="VF226_14240"
     CDS             6520..6849
                     /locus_tag="VF226_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7075194.1"
                     /translation="MKATDLVCAAAVMAAALLLGGPSYAAGDAEAGRQKALRCQTCHG
                     LDGVARIPDAPHIGGQNEFYLINSLKAYKTGLRQNDMMNLIVSDLSDQDIADLAAYFA
                     SLDPKNE"
     gene            6988..7878
                     /locus_tag="VF226_14245"
     CDS             6988..7878
                     /locus_tag="VF226_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075195.1"
                     /translation="MAQSTAAEAVDQAEMVARDAALEQPSSGSAGRRSSASAKSNRTA
                     SDGWRRFTLDVWITDFNSVEFGIYRHSRNRAKSRQFSTDMDIFAEVKENGQRTGVIGY
                     REDLWKQKSSADRRLIIRLFSDTLNWRATMDLMIGRSLQLTLGARGIPVTAFAINTYE
                     DKSIVYLERSANKWPVLPENFSFFVIREGQPVFYRLRHDFFSVGGDYTLLDARSRKVG
                     HIDGRLFTLGGYWNCKVREDHADDKLMSVMKLFAAGIGFNSACRRHIKRLAADVRAGR
                     LVPKLERYEAEYYLNPRRVR"
     gene            8006..9223
                     /locus_tag="VF226_14250"
     CDS             8006..9223
                     /locus_tag="VF226_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7075196.1"
                     /translation="MSGTPGLEALAQGVILSPFTRIARLLEGVAPGHTKIIEMTAGDP
                     KETMPGFIIDKMAEVKHLLGTYPKIRGSDDLRGAIAAWIRRRYGEAVQVDPAREVHPI
                     NGSREGLFYAALPAVGRKPVDGRPVIVLPNPFYHSYLGATYATNCEPYFLNATEATGH
                     LPNLDELERQTDILKRTVAFYLCSPANPQGVVADHAYIRRALELARRYDFMLFFDECY
                     SEIYTGDPPIGGLEVAASTPERFRNIVVFNSLSKRSNLPGLRSGFAAGDGDFLETLAE
                     VRNLVAPQMPGVVQHASAAVWSEEQHVAAIRQAYRTKFDICDELLRGRYGYRRPAGGF
                     FLWLDVGQFGGGEEATVTLWKRCGVKVVPGAFLAQPGPDGLNPGENYVRVALVHDAGT
                     IREALERIVSTLS"
     gene            9240..11765
                     /locus_tag="VF226_14255"
     CDS             9240..11765
                     /locus_tag="VF226_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX7075197.1"
                     /translation="MLQQGYGVDQQRLLPLSVEERLSTWISRCSGAALFLAGLAGWIS
                     LITWSVSDPSLTHTIALGPTRNLLGPGGAIVADLMLQLLGLASTFLVLAPTIWGIELF
                     SSGRITFARTRTMLLAIAVLALAAGCSALPTAANWPLHNGFGGVIGDLLYNLFAGLFA
                     ALVPARAGAIAGLLLFSGGLAAFICCLGLGGWWPSEFWQPGTVVPRPALRTGERRWHF
                     QMPSLFQRTRAPRSREPIEPVLSVPSRKRASEPAWSDEEADRTRRPRAPRASRFEEEF
                     EPGAGRADADGDALDPDLDADEESRRIAERFAPPNAQPGPARAARGPTAGLLGTLALR
                     RGEAGFRRPGVNLLKRPAGGKPGPEFTQTVLRGTARLLEDVLTDFGVKGEIKDIKPGP
                     VVTLFELEPARGTKSSRVIGLAEDIARSMSATAVRVAVVPGRNVIGIELPNTRREMVL
                     LREIFEAEAFRASDARLPLALGKTIAGDPIVVDLARMPHLLIAGTTGSGKSVGVNAMV
                     LSLLYKLSPEDCRLLMIDPKMLELSVYNGIPHLLTPVVTDPHKAVAALNWVVREMEER
                     YKRMAALGVRNIDVFNNRVRHAKSRGDRMRRTVQTGFDRRTGEAVFETEELDLEPMPY
                     IVVVADEFADLMIVAGKEIEAAVQRLAQMARAAGIHLIMATQRPSVDIITGTIKANFP
                     TRISFKVTSKIDSRTILNEQGAEQLLGQGDMLFSPGSGQVIRVHGPFVSDEEVESIAQ
                     FLREQGEPEYVEGVTDGAPDVDEFGEPGSAKNGEGDLYDKAVAIVLRDRKASTSYLQR
                     RLSIGYNRAADLIERMEREGIISPATATGKREILLSGVSSSAA"
     gene            11913..12647
                     /locus_tag="VF226_14260"
     CDS             11913..12647
                     /locus_tag="VF226_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX7075198.1"
                     /translation="MRALSAMAAAGAACLLAVSAASSGPAMAQQAQAAPQPEAADAAS
                     PGWTGTINAAQDGTEAPFTEFQLAAIEKVSNYFNELKNLKGSFLQTDPDKKQMRGKFF
                     VKRPGRFRFDYGAPSLKVIISDGRWLAIQDHDLNTEDVYELDNTPFRLLLREDVDLAR
                     DARIIDVQEAEDVIILTMQDKDPDVPGRITLFLTKQPELTLKEWVTVDAQGLETRVQL
                     SGLDTKEDLDPALFKRENITLKRFQQ"
     gene            complement(12977..13501)
                     /locus_tag="VF226_14265"
     CDS             complement(12977..13501)
                     /locus_tag="VF226_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7075199.1"
                     /translation="MTIELLVEPLRRVALFQGLQPAQLNEIARRSERIQFKAGDTVIS
                     EGQPADAAILIVTGTAKRTEGPSIGDDTFGPGMLLGEMAMLVETEYSSTVVCVEPIRA
                     LKITRERLLEQMSSDISLADHFVAQLSGRLRQLAAEFRKISEVLEPAPQPKRRMRAPA
                     QLAFDPSAQQTAMH"
     gene            complement(13704..14390)
                     /locus_tag="VF226_14270"
     CDS             complement(13704..14390)
                     /locus_tag="VF226_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7075200.1"
                     /translation="MSAARKILIVDDDEDLRETLRDQLTLHAEFDVATAATASAGMEA
                     ARNEQTDLMILDVGLPDLDGREACKALRKAGFKAPIIMLTGNTSDADTILGLDAGAND
                     YITKPFKFAVLLARIRAQLRQHEQSEDAVFTIGKYTFKPASKLLIDEKGSKIRLTEKE
                     TSILKYLYRAGEKVVTRDVLLLEVWGYNTGVTTHTLETHIYRLRQKIERDPSNAELLV
                     TETGGYKLIP"
     gene            14543..15046
                     /locus_tag="VF226_14275"
     CDS             14543..15046
                     /locus_tag="VF226_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7075201.1"
                     /translation="MRSTRGWARIGGLRLPCALGRSGRRVKKREGDGATPVGAFRVRR
                     AFYRPDRLMRPLTRVPLTPLEPDDGWCDAPGDRNYNRKIKHPYPASAERMWREDHLYD
                     VVIVLDHNERPRVQGGGSAVFIHCARPGFPPTAGCIAFRRADLLRLLTRLEAGTVVRI
                     APWMRPA"
     gene            complement(15195..16511)
                     /locus_tag="VF226_14280"
     CDS             complement(15195..16511)
                     /locus_tag="VF226_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7075202.1"
                     /translation="MKLDRLLRRAGGVAIATALTAPPALAQDAAAPMTPTLDTGDTAW
                     MLTSTVLVLMMTIPGLALFYGGMVRKKNVLATVMQSFAATCLMTVLWMLIGYSIAFGN
                     GGSFNAYVGGLEKMLLASLSKDSLTGTIPESVFMTFQMTFAIITPALICGAVADRMKF
                     SSLLVFLAAWSILVYAPICHWVWGGGFLGSDGVLDFAGGTVVHINSGVAGLICAIVLG
                     RRQGYGSENMAPHNLVLTITGASLLWVGWFGFNAGSAAAANATAGMAMAVTQIATAAA
                     ALAWMFSEWIVHRKPSVLGIASGAVAGLVAITPAAGFVAPMGALVIGIAAGVICFLAA
                     TTVKRALGYDDSLDVFGIHGVGGIVGAILTGVFAVEAIGGTPGLLEGNAAQVPVQIYG
                     IIATVAWCALATFVILAVVNVLMGLRVDKATEIEGLDINLHGEVVH"
BASE COUNT         3131 a         5220 c         5458 g         3127 t
ORIGIN      
        1 actgcgggcc ggaggacgat ccgatgcgat gcgcgccagg tcgaggatga ggcgctccgt
       61 cattggcgat tccagcgcgt cattcaatct ggccgccttg agcgtttcgg cgatccgtga
      121 aagggcggtg atcagcttgc ctccagtcca catgcggcat tgcgcctgga gagcgtcctg
      181 gaccttctgc ggaggcgagg gccgaagctg gcgcaggatc tgctctagcg atcctcccgc
      241 gtcgatcgcc gttctcaact tgtgcaacct ctggaaatgc cactgcaggg cagcaatcac
      301 cgactgcggg ccacttcccg aagtgatgac gcgatcgtat tcggcgaggg cgcgattcgc
      361 ctctccagat gccactgcga acaggacctt gtcgatggcg agctccgacg catcaccgac
      421 gacagcctcg acgtcgtcca cgtcgatctg gctcttgcca cgcgcgtaca ggatgagctt
      481 ctccagctcc ccccgggtca tggcccgatc agctcctaaa cggctgagga gcgtctgacg
      541 ggcatccggc gtaatggcga caccagcctc ttgcagggtg tcgcggacaa tcgcttcgag
      601 atcgcgggcc tcgtcgggat agcagatcac cgccgccgcg tgcggcgact tctcgaacag
      661 gagccgcagg gcgtcattcg acctgagatt gcccgcttcg accacgagtg aaccggcaag
      721 agcgccgccc tcgagcagag gcttcaggga cgcggcattc acccgccggc ttgcgcgcgt
      781 atgcacaacc tttgggccac cgaacatggg gatggtctgc gtctcgacga ggagacggtc
      841 ggggtcctgc tcgaggtccg catcctcgat gcgcagaatc tcgccaggcg ggtcactgag
      901 ggcggctagc cgttgtgccg cggcacgggc gcgctcggca acagcgcccc catccgggcc
      961 gtgcaccagg atcgcgcgat agcgggaatc gaaagtcttg agaaatcgat cggcctgaaa
     1021 cgtcttgatg gccgtcatgc cgtacctacg caggcggcca ggcgctcgag gccgcccatg
     1081 atatgcgatg gcttgtcagg ctgtgccggc gaggtatgcc gcgagcctgg tcttgatatc
     1141 ctgagcgacg gtccgcgccg cgcggttctc ggcatcctca cgggccttga cgttcgaata
     1201 gatcgaatcg tagcgctcga acccggcccg gccgtagctc gtgccctcca gaagcacggc
     1261 cttgtccgcg atccgaatga gctggaactt ggcttcgagg ttgtaaacct ggctctgcga
     1321 ctcgccatcc ttgcggacga gagtcgagac cacctgttcg cgaatggcga cgtcgaggtg
     1381 atacttcggc ggcagtccgt ggccaccgcc tgtgctctgg aagaccagct cgtttctgat
     1441 cctttggccg acgcgaccgg gaatggtgcc gaacgaaacg gaggcgagct tcgcttcggt
     1501 gtcggccatg ttcgacgcgc cgtacatcgg gcgaaaggcg gacgcgcctt cacatgcagc
     1561 gagcaccggc ccgaggaggc cagcgaatgc gaccgctctc aaaaacgagc gtcgctccca
     1621 gctcggtgtc gagtcctcct gccccctagg cgacgacatt cacgatcctc tgcggaacga
     1681 cgatcacttt cttcacgggt cgcccttcca gagcacggat gaccggctcc agcttcaagg
     1741 cggcagcctc gacctcggct ttgctcgcgt ttctggcgat cgtcagctcg tccctacgcc
     1801 taccgttcac ctgtacggca atggttatcg tatcgtcaac gagaagggcc tcttcgaccg
     1861 tcggccaggg ctgatccgcg agcaaggtgt tgtatccaag ccgctcccag cattcttcag
     1921 ccaaatgcgg catcatcggg ccgaacatct gaacgaggat ctccgccgcc tcccgaatcg
     1981 cccaggccat gccgggacca tccttgccga gggcggcgtg gagtgtgctg gcaaactcgt
     2041 agatctgcgc cacggccacg ttgaaacgga gggtttcgat gttctgactg accttggcga
     2101 gggccttgtg cgcggcacgg cgcagcgcgt cggcatccgg tccgaactcg ccgggcttgg
     2161 ggctgccctt gggagcagcc ttgggtagcg cctcgtcgat cagccgccag acgcgctgca
     2221 tgaaccggct ggcgccagac acgccggcct cggtccaaat gacgtcccgc tcgggcgggc
     2281 tgtcagagag catgaaccac cgcgcgcagt cggcgccata ggtcgagatg atgtcgtccg
     2341 ggtcgacaag gttcttcttc gacttcgaca tcttctcgac gggaccgatc tgcaccgggg
     2401 cgccggtggc gatctcgtag gcacggcgtt ggtcgccctc gccttcgatc cgcacctcgc
     2461 tgggcaagag ccactggccg ttgcggttac tgtaggtctc gtggatcacc atcccttgcg
     2521 tgaagagacc gccgaagggc tccttcacgt tgaggtggcc ggtggcgacc atggctcggg
     2581 cgaagaaccg ggagtagagc agatgcagga tcgcatgctc gatcccgccg atatactgat
     2641 caaccggcag ccaatacttc gcggcatcca gatcgactgg aacgtcggca tggggtgagc
     2701 agaaccgcgc gaaataccag gagctgtcca cgaacgtgtc catcgtgtcg gtttcgcgcg
     2761 tggcaggctt gccgcaagag ggacagtcga cgtgcttcca ggtgggatgg cgcacgagtg
     2821 gattgccggg aacgtcgaag gtcacgtcct cgggcagctt caccggaagc tgatcctccg
     2881 gcacagggac gattccgcac gcatcacagt ggatgatcgg aatcgggcag ccccagtagc
     2941 gctggcgcga gataccccag tcgcgcaaac ggtacatggt ccgccgctcg cccttgccga
     3001 tctcctccat tcggcgggcg acctccgcct tcgcctcgtc gatcgtgagg ccattgagaa
     3061 agcccgaatt ggcgatccgg ccaggtccgt cataagcctc gccgccgaga tcgaatgtgg
     3121 ctgggtcctg accttcgggt atgacaaccg gtacgatggg caacttgtat gcggtcgcga
     3181 actcgaagtc gcgctggtcg tgggccggac agccgaagat ggcacccgtg ccatagccca
     3241 tcagtacgaa attggcgacg aagaccggca gcttgcggtc ggggatcaat ggatgggtcg
     3301 ccgtgagccc cgtattgaag ccctttttct cgacccggtt caggtcctcc tcggaggtgc
     3361 cgacatggcg gcaatcctcg aggaagccag cgagttcggg attgtccttt gccagctcga
     3421 gcgtcagcgg atggccgggc gagagcgcgc agaagctgcc gccgaagatg gtgtcggcgc
     3481 gggtcgtgaa gacttccagc tcccgccagc ccgcaggcaa ccgctcgccg gacaactcat
     3541 atttcatgag caggccttcg gagcggccga tccaattcgc ttgcatgacg cgcacttttt
     3601 cgggccagcg cgtcaggtcc ttcagggcgt ccaggagctc gtccgcgtat ttggtgatcc
     3661 tgaagtacca gaccggcatc tcgcgggtct cgaccagagc cccggagcgc cagccgcgcc
     3721 catcgatcac ttgctcgttg gcgagcaccg tctggtcgac cgggtcccag ttgaccttgc
     3781 cgacgccgcg ataggcgagc ccctccttgt aaaagtcgag gaaaagcttc tgttgctggt
     3841 gataatattc gacatcacag gtcgcgaact cgcgcgacca gtcgagcgac agccccatcg
     3901 acttgagctg ggcgcgcatg gcggcgatgt tgtcgtaggt ccacttcgcc ggatgcgtct
     3961 tgcgctccat ggccgcgttc tcggcgggca tgccgaacgc gtcccagccc atgggatgca
     4021 gcacgttgaa gccgagcgcg cgcttgtagc gggccagcac atcgcccatg gtgtaattgc
     4081 ggacgtggcc catgtggatc cggcccgagg gataaggaaa catctcgagc acatagcact
     4141 tcggccgcgt gtgctcgttc gatgcctgga agacgttgcg gctttcccag acgtgctgcc
     4201 agtgcttctc tcgggcgggc gcgttgtagc gttcgatggc catggggcga cttcagctct
     4261 cgagtgacgg attgccggcg atatcagcct taaaggtgaa aggcggtcaa gagcagctcg
     4321 gaatcctttc gcttttcgag cggcataagc cgtcgccaat ggcgatgcaa acgacagatc
     4381 gtcccgcatg tgtcgacgga tcccgagaat tcggacatat gagccgggtc aaaacaaggg
     4441 ctcgcatgtg atacaggatg acctgatgag cgcggctgct gaacgactga tgaacattcg
     4501 cgagcggatc acggcggctg cccgcgatgc ggggcgcgag ccgtcgtccg tgaggctcat
     4561 cgcagtgtca aagacgttcg acgcgcccca cgttctgccg gtgatcgaag caggccagag
     4621 gctgttcggc gaaaaccggg tgcaggaagc cagggcgaaa tggccgccgc tcaaggagcg
     4681 tttccccgat ctggagttgc atctcgttgg ctccctgcaa tcgaacaagg cgaaggaggc
     4741 ggtgcaactc ttcgacgcca tccattccat cgaccggccc aagatcgcca gggccgtcgc
     4801 tgacgagatg gcgaagcagg ggcgtcgcct gaagctcttc atgcaggtca atacgggtga
     4861 ggagccgcag aaggccgggg tcatgccgcg cgaggcagca gccctgatcc ggcattgccg
     4921 ggaggaaatg aagctcgata ttgcggggct catgtgcatt ccgccggcgg acgaggagcc
     4981 ggccatccat ttcgcttttc ttgcgaagct cgcctccgag ttcggcctga aagagctcag
     5041 catgggtatg agcgccgatt tcgagacggc gatttccttc ggcgcgactt acgtcagggt
     5101 cggcagcgcc atcttcggtg cacgctgaac cggcgtgatg gtcgccggcg gcatggtcag
     5161 catgccgaaa gtgcctaacg ctgattggtg cgttctgcta acattgaagc gcttggtcgg
     5221 atgggcatga ccgagccgat cgactataaa cccatgcgga ggaaacactt atgcgtgccc
     5281 tctcagtctt tgcggccgct ggcgccgcct tgatcgcact acccgctgtc gctcagcagc
     5341 ccagtgccgg caatctcgaa aagctctcga aattccagtc gaccggcaca ccgatcgaca
     5401 ttccccagat tccgcagcaa ggccccaagg ccgatcagat ccgcaagaac ctgaaaaaca
     5461 tcaaactgcc gcccggcttc aaaatcgatc tttacgctct tgtgcccgat gcacgccata
     5521 tggcggtcgg cccgcaaggt gtcgtgacat tcgtcggcac ccggaagaac aaggtctacg
     5581 ccgtcacgga tcgggccaag atgggcgtcg ccgaggaggt gaaggagttc gcgccctcca
     5641 tccagtttaa aattccgaat ggcgtctgct tttcacccga cggcttcctg ttcattgccg
     5701 agcagaaccg ggtgctcgtc ttcccggcag cagagttctt ctacgagagc cccgacgtcg
     5761 ctgcgttcgt ggtcgtgaaa cagggcgagc tgattccgcc cgaggaagaa agcttcaatc
     5821 ataccgctcg tgtctgccgg atcgggcccg acgacaagct ctacatcacg ctcggccagc
     5881 ccttcaacgt cttcgaaaag gagaagatgg acctctacaa gcggaccggc atgagcggta
     5941 tcatccgcat gaaccgcgag ggacggcagc gggaggtctt cgcaaccgga gtccgcaatt
     6001 ccgtcggcat ggatttcaat ccgaaggaca agtcactctg gttcaccgac aaccaggtgg
     6061 acggcatggg tgatgaaatc ccgccgggcg agctgaatcg cgccgaccgg gccggcctgc
     6121 acttcggctt cccgtggtac ggcggaggga aggtgcgcac ggtggagtac aaggacagcg
     6181 agccaccggc caatgtcgtt ttcccgcagg tcgagatgga cgctcacgcc gcggatctcg
     6241 gcatgacctt ctatacgggc aagcagtttc cggagaaata ccgcggcggc atcttctcgg
     6301 cgcagcacgg ttcctggaac cgcaccaatc ctgtcggcgc ccgggtgatg ttcacgagcc
     6361 tgaagccaga cggtacggct gacaagaccg aagtcttcgc cgagggctgg ctgacgccca
     6421 atgcggagta catgggccgc ccagtggacg tcgcgcagct ccgcgacggg tcgatcctcg
     6481 tgtcggatga ctttgccggg gctctctatc ggatcagcta tgaaggcaac tgatcttgtc
     6541 tgtgcggcgg ccgtcatggc cgccgccttg cttctggggg gaccctcgta cgcggccgga
     6601 gatgccgagg caggtcgcca gaaggcgctg cggtgccaaa cctgccacgg cctcgacggg
     6661 gtcgccagaa ttccggatgc ccctcacatc ggcggccaga acgagttcta tctcatcaac
     6721 agcctgaagg cctacaagac gggtctgcgc caaaacgaca tgatgaacct catagtctct
     6781 gatctgagtg accaggacat tgccgacttg gcggcttact tcgcgagcct cgatccgaag
     6841 aacgagtagg acgcgcgtca tgggccgctg gccggcggcc cggcagtttt ccagatatga
     6901 attccggaac cggtcctctg atcccgcttg gcgatgctag ggtctgaaaa gtgattcgcg
     6961 catcgcatct cgggcgaggg attgggtatg gcgcagagca cagcggccga agctgtcgac
     7021 caggcggaga tggtggcgcg ggatgcagcg ctggagcaac caagttctgg ttctgcaggc
     7081 cggcggagta gcgccagcgc gaagtcgaac aggacagcct ccgatggctg gcggcgcttc
     7141 acgttggacg tctggatcac cgatttcaac tcggtcgagt tcggcattta tcgccattcc
     7201 cgcaacagag cgaagtcgcg tcagttctcg accgacatgg acatctttgc cgaggtgaag
     7261 gagaacggtc agcgcaccgg ggtcatcggc taccgcgagg acctttggaa acagaaatcc
     7321 agcgcagacc ggcgtctgat catcaggctc ttttccgaca ccctgaactg gcgcgccacg
     7381 atggacctca tgatcgggcg cagtctgcaa ttgacgctcg gcgcgcgcgg catcccggta
     7441 acggccttcg cgatcaatac gtacgaggac aagtccatcg tctatctgga gcggtcggcc
     7501 aacaaatggc ccgtgttgcc ggaaaacttc tcgttttttg tgatccggga gggccagccg
     7561 gtcttctacc gcctacggca cgatttcttc agcgtgggcg gcgactacac actgctggat
     7621 gcgcgaagcc gcaaggtcgg acacatcgat ggccgtctct tcacgctcgg cggatactgg
     7681 aattgcaagg tccgggagga ccatgccgat gacaagctga tgtccgtgat gaagctgttt
     7741 gctgcgggga tcggcttcaa ttcggcatgt cgacggcaca tcaagcggct cgccgccgac
     7801 gtgcgtgccg gcaggctggt gccgaagctg gagcgctacg aggcggaata ctatctcaat
     7861 ccccgccgag tccggtgagt ttcgggtccc tgtctgcacc cgcactgatc tggaacctgt
     7921 caccagcgct cgtcccgcac tggcatccgc cgcaatcctc gactagacgt cccatcagcg
     7981 gatcctagta agggacgaaa tcgaaatgag tgggactccc ggcctcgagg ccttggcgca
     8041 gggcgtaatc ctgtcgccgt tcacgcggat tgccaggctc ctcgaaggcg tggcgcctgg
     8101 gcataccaag atcatcgaga tgaccgcggg cgaccccaag gagacgatgc ccgggttcat
     8161 catcgacaag atggccgaag tgaagcacct gctcgggacg tatccgaaga tccgcggctc
     8221 ggatgatctt cgcggcgcca tcgcggcctg gatccggcgc cggtacggcg aggcagtgca
     8281 agtggacccg gcacgcgagg tccatcccat taacggctcg cgcgaaggtc tcttctacgc
     8341 tgcacttccg gcagtcggac ggaagccggt cgatggccgg ccagtgatcg tgctgcccaa
     8401 cccgttttat cactcctatc tcggagccac ttacgcaacg aactgcgagc cctatttcct
     8461 gaacgcaact gaggccacgg gtcatctgcc caacctcgat gaactggagc gccagacgga
     8521 catcctgaaa cgtacagtgg ccttctatct ttgctcgccg gccaatccgc agggcgtcgt
     8581 cgcagaccac gcctatatcc gcagggctct cgagctggcg cgccgctacg acttcatgct
     8641 gttcttcgac gagtgctatt cagagatcta tacgggtgac ccacccattg gcgggctcga
     8701 ggtcgcagct tcgacgccgg agcgcttccg caacatcgtc gtcttcaact cgctttcgaa
     8761 gcggtcaaat ctgccgggcc tgcgctctgg gttcgccgcc ggcgacggcg atttcctgga
     8821 gactttggcc gaggtacgca atctcgtggc accgcagatg cccggggtcg tccagcatgc
     8881 ttccgcggcg gtctggtcgg aggagcagca cgtcgccgcg atccgacagg cctatcgcac
     8941 caaattcgac atttgcgacg agctgctgag aggccgctac ggataccgca ggccggctgg
     9001 cggcttcttt ctgtggctcg atgtcggcca gttcggcgga ggtgaagaag ccacggtaac
     9061 cctttggaaa cgctgtggtg tgaaggttgt gcctggtgca ttcctggcac aacctggccc
     9121 ggacgggttg aatcccggag agaactacgt gcgcgtcgct ctcgtgcatg acgcgggcac
     9181 gatcagggaa gcacttgagc gcatcgtgtc caccctttcc taggtttcgc cgggcgccca
     9241 tgttgcagca aggctacgga gtcgatcagc agcgtctgct tccactttcg gtggaagagc
     9301 ggctttcgac ctggatcagc cgctgcagcg gagcggccct gttccttgcc ggactggcag
     9361 ggtggatcag cctgataacc tggtccgtca gcgatccgag cctgacgcac acgatcgccc
     9421 tcggtccgac gcgcaatctt ctcgggccgg ggggcgcaat cgtcgctgac ctgatgctgc
     9481 aattgctggg cctcgcgtcg acgtttctcg tcctggcacc gaccatctgg gggatcgagt
     9541 tgttctcgag cgggcggatc acgtttgccc ggacacgcac gatgctgctg gcgatcgccg
     9601 tgctcgccct ggccgcgggg tgctcggcgc tgccgaccgc agcgaattgg ccgctgcaca
     9661 atgggttcgg cggggtcatc ggggatctgc tctataactt gttcgctgga ttgtttgcgg
     9721 cgctcgtccc cgcgcgtgcc ggggcgattg caggtctcct gctgttttca ggagggctcg
     9781 ccgcgttcat ttgctgtctc ggcctcggcg gctggtggcc atccgagttc tggcagccag
     9841 gaacggtggt gccgcggccg gcgctacgga ctggggagcg gcgctggcat tttcagatgc
     9901 cgagcctgtt ccagcgcacg cgcgctcctc gctcccgtga gccgatcgaa ccggtgcttt
     9961 cggtgccatc gcgaaagcgt gcctccgaac ccgcatggtc ggatgaggaa gctgaccgaa
    10021 cccggcgccc acgcgctcct cgggcgagcc gatttgaaga agaattcgaa cccggcgccg
    10081 gccgtgcgga tgccgacggg gacgcgctcg acccggacct cgatgccgac gaggagagcc
    10141 gccggatcgc cgagcggttc gcgccaccga atgcacagcc agggccggcg cgggccgcgc
    10201 gaggccccac ggccggcctg ctcggcaccc tggcgctgcg gcggggcgag gcgggcttcc
    10261 gcaggcccgg tgtcaatctg ctgaaacggc ctgccggcgg caaacccggg ccggagttta
    10321 cgcaaacggt gctccgcggc accgcccgcc tgctggagga cgttctgacg gatttcggcg
    10381 tgaagggcga gatcaaggac atcaagcccg gcccggtcgt gacgttgttc gagctggagc
    10441 cggcgcgcgg cacgaagtcc tcgcgcgtga ttggcctcgc cgaggatatc gctcgctcga
    10501 tgagtgcgac tgcggtgcgc gttgccgtcg tgcctggccg caacgtgatc ggcatcgagc
    10561 tgccgaacac ccggcgcgag atggtgctgc tgcgggagat cttcgaggcg gaggccttcc
    10621 gcgcgagcga cgcccggctg ccgctggcgc tcggcaagac catcgccggc gatccgatcg
    10681 tggtcgatct cgcccgcatg ccgcatctcc tgattgccgg caccaccggc tccggcaagt
    10741 cggtcggcgt caatgccatg gtgctgtcgc tgctctacaa gctgtcgccc gaggattgcc
    10801 gcctgctgat gatcgatccg aagatgctgg agctgtcggt ttacaacggc atcccgcatc
    10861 tgctgacgcc cgtggtcacc gatccgcaca aggcggttgc cgcgctcaac tgggtcgtgc
    10921 gtgagatgga ggagcgctac aagcggatgg cggccctcgg cgtccgcaac atcgacgtct
    10981 tcaacaaccg tgttcgccac gccaaatcgc gcggcgatcg catgcggcgc accgttcaga
    11041 ccggtttcga ccggcggacg ggcgaggcgg tgttcgagac cgaggagctc gatctcgagc
    11101 cgatgcccta catcgtcgtc gtcgccgacg agttcgcgga cctgatgatc gttgccggca
    11161 aggagatcga ggcagcggtg cagcgcctgg cgcagatggc ccgagcggct ggcattcacc
    11221 tcatcatggc gacgcagcgc ccctcggtcg acatcatcac cggcaccatc aaggccaatt
    11281 tcccgacgcg gatcagcttc aaggtgacat cgaagatcga cagccgcacg atcctcaacg
    11341 agcagggcgc cgagcagctt ctcggccagg gcgacatgct gttctcgccg ggctcgggcc
    11401 aggtgatccg cgtccatggg cccttcgtct ccgacgagga ggtggaaagc atcgcccaat
    11461 tcctgcgcga gcagggtgag cctgaatacg tcgagggcgt caccgatggt gctccggacg
    11521 tggatgaatt cggagagccc ggaagcgcca agaacggcga aggcgatctc tacgacaagg
    11581 ccgtcgcgat cgtcctgcgc gaccgcaagg catcgaccag ctatctgcag cgccggctct
    11641 cgatcggcta caaccgggcg gccgatctca tcgagcgcat ggagcgggag ggcatcatca
    11701 gcccggcgac tgccacgggc aaacgagaaa ttctgctgtc gggcgtatct tcgtcagctg
    11761 cttgaatgct cggacatcga catattcatc cacaactcgg cccgcgcacg acgttgtgtg
    11821 agcatatccg ggagctagac tgcctacatc tggtctcaat gttgcgacaa tcggttatca
    11881 aggaatcacc tgggctaaac acagggggcg ggatgcgcgc actatcagct atggccgcgg
    11941 caggcgccgc ctgcctgctc gcagtatccg cggccagcag tggtccagcc atggcgcagc
    12001 aggcccaggc ggcgcctcag ccggaggctg cagatgcagc cagccctggc tggacgggca
    12061 cgatcaatgc ggcgcaagac gggaccgagg cgccctttac agagttccag ctcgccgcca
    12121 tcgagaaggt gagcaactac ttcaacgagc tgaagaacct gaaaggctcg tttctgcaga
    12181 ccgaccccga caagaagcag atgcggggca agtttttcgt gaagaggcct ggccggttcc
    12241 gcttcgatta cggtgcgccc agcctcaagg tgatcatctc ggacggccgg tggctggcca
    12301 tccaggacca cgacctcaac acggaagacg tctacgagct cgacaatacg ccctttcggc
    12361 tattgctccg cgaggacgtg gacttggcgc gcgatgcgcg catcatagac gtccaggaag
    12421 ccgaggacgt gatcatcctg acgatgcagg acaaggaccc ggacgtgccg gggcgtatta
    12481 cgctgttcct gaccaagcag ccggagttga cgctcaagga gtgggtgacg gtggatgcgc
    12541 agggtcttga gacccgggtt caactttccg gcctggatac gaaggaggat ctcgatccgg
    12601 ccctgttcaa acgggagaac atcacgctga agcgattcca gcagtgatct ccaggcctgt
    12661 ggattgttcg ccgcgggcaa atgaaacttg tgcagaatgg gatcaagcct gatcacgttt
    12721 tcgtgaaaag gtgaggcacc ctctattgca ttcgtacgca ttaagcttaa ctaggaagct
    12781 gacaggcggc gtcaccctgt atcggtagtg ccccccgtct agccgaagac gctgcctgcg
    12841 gcgtcggagt ccctcccggc gccagcgctg atagcgctcc ttgcgcccaa ctcctccccc
    12901 cggagttggg cgcttttcat tgcgccacgc gatttgggcg actgctcgcc ggggcttggt
    12961 ggtgatcgaa gctcgcctaa tgcatcgcgg tctgctgggc agacggatcg aaggccaatt
    13021 gcgctggagc cctcatacga cgctttggct gtggcgcggg ctccaagact tcgctgatct
    13081 tgcggaactc ggcggcaagc tgccgcagcc ttccggacag ctgtgcgacg aaatggtcgg
    13141 cgaggctgat gtctgaactc atctgttcga gcaagcgctc gcgtgtgatc ttcaaagcgc
    13201 ggatgggctc cacgcagacg accgtggaag aatactcggt ctcgaccagc atggccattt
    13261 cgccaagcag cattccaggt ccgaacgtgt cgtcaccgat gctcggaccc tctgtgcgct
    13321 tcgccgtgcc tgtgacgatc agaatcgcgg catctgctgg ctggccctcg ctgatgacgg
    13381 tgtcgccggc cttgaattgg atccgctcgg accgccgggc aatctcattg agctgggcgg
    13441 gctgcaatcc ctgaaagagg gcaacgcggc gtaacggctc gacgagcaac tcgatggtca
    13501 tgttctgctc tccccatgca gcccggcgaa ggtgtgtagc gaccggcgga aacaggtgcc
    13561 gatttatcgc acggggagga cagatgattc ggcggttcgg gtaaagtgtg cgggcggtga
    13621 attctgaata aaaaccgggg agtgctttcg cgtcgcaaat ggctgccatt ctgctcaggc
    13681 aaagcaattc aggccgccac ccgtcaaggg atcagtttgt aacccccggt ttcggtcacg
    13741 agcagctcgg cgttggaggg atcgcgctcg atcttctgcc gcagcctata gatgtgcgtt
    13801 tcgagcgtat gcgtggtcac gccggtattg tacccccaga cctcgagtag aaggacgtcc
    13861 cgggtgacga ccttttcgcc ggcgcggtag aggtacttga gaatggaggt ctctttctcg
    13921 gtcaggcgga tcttcgagcc cttctcgtcg atgagcagct tcgacgcggg cttgaacgtg
    13981 tatttgccga tggtgaagac ggcatcctcg ctttgctcat gctggcggag ctgggcacgg
    14041 atgcgcgcca gcagcacggc gaacttgaat ggcttggtga tatagtcgtt ggcaccggca
    14101 tcgagaccca ggatcgtatc ggcatccgag gtattgcccg tgagcatgat gattggtgcc
    14161 ttgaaacccg ctttgcgcaa cgccttgcaa gcctcgcgtc cgtccagatc aggcaggccg
    14221 acgtcgagga tcatcaggtc ggtttgctcg ttgcgtgcgg cctccatacc agccgaggcg
    14281 gtggcggcgg tcgcgacgtc gaactctgcg tgcagggtga gctgatcccg tagggtctca
    14341 cgaaggtcct cgtcgtcatc gacgatgaga atcttgcgtg ccgcgctcat ttccgtgtca
    14401 ctcccgcatc ttagaaattt gtgaacaatg tatcaccgga gaggctggcg aaacagtgtt
    14461 tagggcatct gtgagaaggg cctgtgaagg ggaccgaagc taccccagga caatggacga
    14521 gctccacgtc agcgcgctat cgatgcgatc gacccgcggc tgggcgcgca ttggcgggct
    14581 caggctgccg tgtgctctgg gccgatcggg gcgacgtgtc aagaagcgcg aaggcgatgg
    14641 tgccacgccg gtcggcgcct tcagagtcag gcgggccttc taccggccgg atcgcctgat
    14701 gcgccctttg actcgggtgc cccttacacc gcttgagccc gacgatggct ggtgcgatgc
    14761 gcccggtgac cggaattaca atcgcaagat caagcatccc taccccgcga gtgcggaacg
    14821 gatgtggcgc gaggaccacc tctacgacgt cgtgatcgtg ctcgatcaca acgagcgccc
    14881 gagggtgcag gggggcggca gtgcggtgtt catccactgc gcgagaccgg gcttcccacc
    14941 gactgccgga tgtatcgcgt tccggcgagc ggacctgcta cgccttctca cgcggctgga
    15001 ggcgggcacg gttgtccgca tcgcaccttg gatgcgcccc gcctgatggg ctccgggacc
    15061 acttctgcct cgccaaaaga gacgcccggt tcgcggaaac gaaccgggcg agttggtcta
    15121 gggagggaat gaaacgacca ttacatggtg atcgcgccgg ttctgttcca gaggccggcg
    15181 cacatcgaga gcgcctaatg gacgacctcg ccgtgcaggt tgatgtcgag gccttcgatc
    15241 tcggtggcct tgtcgaccct gagacccatg aggacattca cgaccgcgag gatcacgaag
    15301 gtggcgagcg cacaccaggc gaccgtggcg atgatgccgt agatttgcac cggaacctgg
    15361 gcggcgttgc cttccagcag gccgggcgtg ccgccgatcg cctcgaccgc gaagacgcca
    15421 gtcaggatcg cgccgacgat gccgccgacg ccgtggatgc cgaacacatc gaggctgtcg
    15481 tcgtagccga gggcccgctt caccgtcgtc gcggcgagga agcagatcac gcctgcagcg
    15541 atgccgatga caagggcacc catcggagcc acgaacccgg cagcgggcgt gatggcgacg
    15601 aggccggcaa cagcgccaga tgcaataccg agcacgcttg gcttgcggtg gacgatccac
    15661 tccgagaaca tccaggcgag cgccgcggca gctgtcgcga tctgggtaac ggccatagcc
    15721 attccggccg tggcgttggc tgctgcggcc gaacccgcgt tgaagccgaa ccatccgacc
    15781 cacaggagcg acgcgccggt gatcgtcagc acgagattgt gtggggccat gttttccgag
    15841 ccatagcctt ggcgccggcc gaggacgatg gcgcagatga ggccggcgac gcctgagttg
    15901 atgtggacca cggtgccgcc ggcgaaatcg agcacgccgt cggagcctag gaagccgccg
    15961 ccccagaccc agtggcagat aggggcatag acgaggatcg accacgcggc caggaatacc
    16021 agcagactgg agaacttcat ccgatccgcc accgcaccgc agatcagggc cggcgtgatg
    16081 atggcgaacg tcatctggaa ggtcatgaag acggattccg gaatggttcc agtcagagag
    16141 tccttgctca ggctcgcgag gagcatcttc tcgaggccac cgacataggc gttgaatgat
    16201 ccgccgtttc cgaaagcaat cgagtagccg atcagcatcc acagcacggt catcaggcag
    16261 gtggcggcga agctctgcat caccgtggcg aggacattct ttttccgcac catgccgccg
    16321 tagaagagcg caaggccagg gatcgtcatc atcagcacga gcacggtgga ggtgagcatc
    16381 caggccgtgt cgcccgtgtc tagtgtcggc gtcatcggcg ccgctgcatc ttgtgccagc
    16441 gctggtgggg cggtcagcgc cgtcgcgatc gcgacacctc cggccctgcg caaaagcctg
    16501 tcgagcttca tttgcggaaa tcccttgttg gtgaatgacg tgtccaggcg agcggattac
    16561 gtgtcagcct gcgctgctgg agagcgtcgc tgtcggcatg tttcagcgcg gatcccgtcg
    16621 gcgtgcgcga cggatgagat gagcttcatt tcggcgaccc tctcggttgg ttacacggga
    16681 gcccggaaag cccggggcgc gagagccttt ccctttgaac gggcgatggc ctcgagccga
    16741 agagagtcaa gacgcgtgcc agatgactaa cgcagccgta agcacctgaa aaatcgatca
    16801 gattcggctc ttgtgaggag agcgagaggc cgcaggccgc gcggttctgc ttaaaagttc
    16861 ggcaagctgc tagatttgcc tgctaactga ccacgtagct ggtcgtgcgc tggcgaacga
    16921 gcccttcctg cgcgac
//
LOCUS       DASVDU010000225         9655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1260092, whole genome shotgun sequence.
ACCESSION   DASVDU010000225 DASVDU010000000
VERSION     DASVDU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9655
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260092"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF226_14285"
     CDS             <1..531
                     /locus_tag="VF226_14285"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX7075203.1"
                     /translation="AIQFLCLAMFMIGCMLYLAIIPLIFYRLTFIKLEAYEFEPPYWI
                     NMGAVAISALAGSILLLRTDGWTVLLEFRPFLKGFTVFFWSVATWWIPLLITLMIWRY
                     LVRRDRLQYEPQFWGMVFPLGMYTTCTYQLSHAVELPFLEAIPDLFIYVALIAWVITF
                     AGFIQHVSRLIAHTRQ"
     gene            complement(553..921)
                     /locus_tag="VF226_14290"
     CDS             complement(553..921)
                     /locus_tag="VF226_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075204.1"
                     /translation="MLQVRPWKNRALFRFFETEIRAWYGLEPLGKVFWGYGVLASTIM
                     AVLYALALVQQRVIVQQLLLIFFGAYTVWIITAVWRCAQSAPPIWQTLARSLTVAWAA
                     NATLVVGFLELDFIGRFLAM"
     gene            complement(1025..1423)
                     /locus_tag="VF226_14295"
     CDS             complement(1025..1423)
                     /locus_tag="VF226_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HEX7075205.1"
                     /translation="MALIEWRREFETGIAEVDHEHRELVDLINALHAEIGANASKAVV
                     SGFLGEVFAKISAHFALEETVMRKHRYDEYAAHKSDHEKLLDDIRDIMDAHESGLFVD
                     YEAALGRAVRDWFVDHFKTKDARLHRMLGV"
     gene            complement(1430..1936)
                     /locus_tag="VF226_14300"
     CDS             complement(1430..1936)
                     /locus_tag="VF226_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075206.1"
                     /translation="MASEIMRYFGQALIYAAIALAIGAFSSWPTLEYFPADKAQIKIS
                     FAHGAKRVDECRRLTPEEIAKLPPRERRPHTCGRERLPTRVQLLVDGVAIYDEVVEPT
                     GLSRDGPARIYRKFAVTPGRHEITARLRDSNRTEGFDYERRVTLELAPLQNLAIDFSS
                     DQGTFIIR"
     gene            complement(1929..3503)
                     /locus_tag="VF226_14305"
     CDS             complement(1929..3503)
                     /locus_tag="VF226_14305"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7075207.1"
                     /translation="MKVLRALIRRGFDIAEAGLSKIFPPEWNPLLNLGALGFFFYWII
                     TVSGIYLFIFFETTVHNAFDSVEYMTVDQWYAAGVMRSLHRYASDGLVVVMVAHLLRE
                     FGLDRYRGVRWFSWVTGVPVLVLVFVAGITGYWMVWDTLAQYVALVTTEWLDKLPIFG
                     EPIARNFFAPDTLESRFFTLMVFMHIAVPLIALIILWVHLQRVTKPRINPPRGLAIGV
                     LVALLTLSLVHPATSQGPADLAKVPATVGLDWFYLPLYPLLDRWPGPVTWGASGALLL
                     ILIAMPWLPPLRKPAAAVVDLANCNGCTRCFNDCPYNAITMGSRTDGRPFERQAIVNP
                     ALCVGCGICAGACPTSTPFRTASDLIPGIDLPDHSISALRDKVRAAAADLRGRSRILV
                     FGCEHGAPLRDLASNTASVSVRCVGQVPPSFIDYVLSRNLADGVVLAGCAENSGHARI
                     GMKWTEARLARTRDPHLRARVPADRLKVLWAGRLGRKKLGSLLQEFIRELDHMPAPQP
                     RPEVRRMAKVEEFIRG"
     gene            complement(3503..4309)
                     /locus_tag="VF226_14310"
     CDS             complement(3503..4309)
                     /locus_tag="VF226_14310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7075208.1"
                     /translation="MSRPGWPLIMALSLFASLKTVSPAAASNAENAIALSQAALGQEL
                     PDLAFTDTEGRTVKLSDYRGKPLLVSLIYTGCADVCPTLIESLYPAVKTARQVLGEDS
                     FNVVTIGFDTRNDTPSRLRLFARTRGIDLPNWKFLASDEESLDALAKAVGFGIYSRAG
                     GFDHLAQVSLIGADGRVRQQIYGAVFEPPVIVDPLKAVVLGRNKPLASLEGLVDRIRW
                     FCTIYDPKSGRYYFSYSLFISIGIGLASFSLIIVALIREWRRSSGHGVSP"
     gene            complement(4418..6154)
                     /locus_tag="VF226_14315"
     CDS             complement(4418..6154)
                     /locus_tag="VF226_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7075209.1"
                     /translation="MAIATAETGVIGGVAARFRTCPASGLKIDLAAERLIKANAVAAV
                     VWLAVGGLLGLMVALTRWQAVHLLPADAFYMVLTAHGLDVLLLWIVFFEMAVLYFASA
                     VLLNCRLAAPKVAWIAFALMMGGGLLTNYTVLQGQSSVMFTSYPPMQAEPNFYLGLIL
                     FAVGALLGCFVFLGTLVIAKEEKTYEGSIPLVTFGALTACIIAIFTLASGAIVLVPTW
                     LWSIGYISEINTVMYKLVWWAMGHGSQQVNVSAHVAIWYAIMAILLGAKPLSEKVSRG
                     AFFLYILFLQLASAHHLLVEPGVSAEWRVFNTSYFMYLAVLGSMIHGMTVPGALEAAQ
                     RRNGYTRGAFEWLRKAPWGNPAFAGMFLSLVMFGFLGGISGVVLGTEQINIMLHNTLY
                     VPGHFHATVVTGTTLAFMAVTYLLLPLIFQRQVIWPKLAKWQPYLFGIGAAGISLFMM
                     GAGTLGVARRHWDVGFTDAILSFDYPAAAFLMLTLNGISAIIAATGGVLFVVIVVASV
                     LWGKPVDKSNIIESLGPMPQRQLSDAVSTYGNAGTWKLPGTYILVTVFFTAFVLYYFV
                     NWKYLSQLWPMG"
     gene            complement(6166..6735)
                     /locus_tag="VF226_14320"
     CDS             complement(6166..6735)
                     /locus_tag="VF226_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075210.1"
                     /translation="MAIHPPEQRVWWNEPVEKDELIWIAIAFLWGLLMFGMMVYWHIE
                     GRQNLSTQAYRIDPAAYEKRVDEFAEKFKVREEGETGIPVVRPPAGGDVYMLARLWEW
                     WPVLELEKGQSYRLHLSSLDWQHGFSLQPVNINIQVHPELEHVVTMTPTEAGTFSIVC
                     NEFCGLGHHTMVGRIHVVEPGTAARGAPQ"
     gene            complement(6748..6927)
                     /locus_tag="VF226_14325"
     CDS             complement(6748..6927)
                     /locus_tag="VF226_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075211.1"
                     /translation="MANIGQHAPERPPTEPERIPAMQRALDNPFLLLFLGVTIPTVFY
                     ILWGLIELTHIPIAK"
     gene            7232..8884
                     /locus_tag="VF226_14330"
     CDS             7232..8884
                     /locus_tag="VF226_14330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7075212.1"
                     /translation="MRDLSQHLVPVVGVLAFIVAVTSVILIWQARRQAVYEGKIKAEL
                     VSRVASEQTLRAFQTTELALQRLGYLLEHGPPLPENDEGFAREMQAAIRDLPHIRAIH
                     VVAPDGFIIHATTFPHSTRIRVSDRDYFVAQLKSTPEGRGTGVYIGQPIRSRGWEGWF
                     IPVSRRINRRDTGFSGVIVAAIEPKYFEDLFRDLNLESGDALALFNTDGNLIARFPKS
                     PGLIGRSWSQLRLPSGGNQLSPTHAGLRLGQSLENKPVVISYRRLATYPVIAAVLLGQ
                     RELLAHWRQVALEWGVSSVALIVLMLAFAVVLQRRRQERQTAEARALAIQRFETVGHT
                     TSGIAHDFNNVLAVVAASLRLLRNGKPPDQVLPAAEQALERGQRLTSQLLGFARRHSL
                     EIKPHDIDQLVVALQPMLKQAAGPKCTVSYRLNSSGTRCHADQAQFDAAMLNLVVNAA
                     QAMDRGSIEIITEPVMLDRRSKTALKPGRYVSVKVKDDGCGIPADALGRVFEAFYTTK
                     KESGTGLGLAQVYTFMHYVHGDVRIESQVGVGTTVELLFPCA"
     gene            9195..9530
                     /locus_tag="VF226_14335"
     CDS             9195..9530
                     /locus_tag="VF226_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7075213.1"
                     /translation="MRPDENRAGAEHPLLIPAVRASASLLHFTIDGTPATCSAGRTVL
                     AAILLSRTSLNTNEASGEPRAGFCLMGACQDCWVWLEDGTRLRACTTPIAEGMAILTE
                     PPTRRQRNG"
     gene            9523..>9655
                     /locus_tag="VF226_14340"
     CDS             9523..>9655
                     /locus_tag="VF226_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7075214.1"
                     /translation="MADVVIVGAGPAGIAAADVLVSNGISPTVIDEGARPGGQAYRTS
                     "
BASE COUNT         1987 a         2966 c         2917 g         1785 t
ORIGIN      
        1 gccatccagt tcctctgcct ggcgatgttc atgatcggct gcatgctcta tctcgccatc
       61 attccgctga tcttctatcg cttgacgttc atcaaattgg aggcctacga gttcgagccg
      121 ccctactgga tcaacatggg cgcggtcgcg atttcggccc ttgccggatc gatcctgctg
      181 ctgcgaaccg acggctggac cgttctcctc gagtttcggc cgtttctcaa aggcttcacg
      241 gtctttttct ggtccgtggc gacatggtgg atcccgctgc tgatcacgct gatgatctgg
      301 cgctatctcg tcaggcggga cagattgcaa tacgagccgc aattctgggg catggtgttt
      361 ccactgggca tgtacacgac ctgcacgtat cagctttcgc atgccgtgga gctgcccttt
      421 ctggaggcca ttccggacct tttcatttac gtggcgctga tcgcctgggt catcaccttc
      481 gcaggattta ttcagcacgt ttcacgcttg atcgcgcaca cgcgacagtg acgtgcccct
      541 ccagcgcgtt cctcacatcg ccagaaagcg acctatgaag tcgagctcca gaaagccgac
      601 gacgagggtg gcattggcag cccaggccac ggtcaggctg cgtgcaaggg tttgccagat
      661 cggcggagcc gattgcgcgc agcgccatac cgcggtgatg atccagaccg tataggcacc
      721 gaagaaaatc agcagcagtt gctggacgat gacccgctgc tggacgagag caagggcata
      781 gaggacggcc atgatcgtgc tcgccagcac gccatagccc caaaagacct tcccgagcgg
      841 ctcgagccca taccaagccc gaatctccgt ctcgaagaaa cggaacagcg ctcgattctt
      901 ccatggccgg acctgcagca tgatgacgac tctgcagaat ccaggccctc ttcgcaatgc
      961 gcaccggccg ttaagccgca gcgcacggcc gacaatgtag ccggtgccgt cggtgtctcc
     1021 cggttcagac acccagcatc cggtgcagac gggcatcctt cgtcttgaag tggtccacga
     1081 accaatcccg cacggctcgg ccgagcgccg cctcgtaatc gacgaaaagg ccactctcgt
     1141 gggcatccat gatgtcgcgg atgtcgtcga gcagcttttc atggtccgac ttgtgcgccg
     1201 cgtattcgtc gtaccggtgc ttgcgcatga cggtttcctc gagcgcgaag tgggccgaga
     1261 tcttcgcaaa gacctcgccg agaaagccgc tcacgaccgc cttcgacgcg ttcgccccga
     1321 tctcggcatg cagtgcattg atgagatcga cgagctcccg gtgctcatga tcgacctccg
     1381 caatgccggt ttcgaattcg cgccgccact cgatgagtgc catgatggtc taccggatga
     1441 tgaacgtgcc ttgatcgctg ctgaagtcga tcgccaggtt ctgcagcggc gccagctcga
     1501 gggtcacccg gcgctcgtaa tcgaaccctt cggtcctgtt gctgtcgcgc aagcgcgcgg
     1561 tgatctcgtg acgccccggg gtcaccgcaa atttccgata gatacgggcg ggaccgtcgc
     1621 gcgacaggcc ggtcggctcg acgacctcat cgtagatcgc aacgccatcg accagaagct
     1681 ggactcgcgt cggcagccgc tcccgtccgc aggtgtgcgg gcggcgctcg cgaggcggta
     1741 acttggcgat ctcctcaggc gtcaaccgcc ggcattcgtc gacgcgtttg gcgccgtgcg
     1801 cgaagctgat cttgatctgt gccttgtcag cagggaaata ctccaacgtc ggccagctcg
     1861 agaaagcacc gatggcgagc gcaatagccg cataaattag ggcctggccg aagtatctca
     1921 tgatctcgct agccacggat gaactcctca accttggcca tccgcctcac ctcgggccgg
     1981 ggctgcggcg ctggcatgtg atctagctcg cggatgaatt cctgaagcag cgagccgagc
     2041 ttctttcggc caagtcgtcc ggcccacaac accttgagcc gatcggccgg tacgcgcgcc
     2101 cgcaggtgcg ggtcacgtgt ccgcgcaagg cgcgcttccg tccacttcat gccgatgcga
     2161 gcatgaccgc tgttttccgc gcagcccgcc agcacgaccc cgtccgcaag gttcctgctc
     2221 agcacgtagt cgatgaacga gggcggcacc tgcccgacgc agcgcacgct gacggacgcg
     2281 gtgttggagg caagatcgcg caacggcgct ccgtgctcgc atccaaacac caggatgcgg
     2341 ctcctgccgc gcaggtccgc agccgcggca cgcaccttgt cgcgcagcgc gctgatgctg
     2401 tggtcaggca aatcgatgcc ggggatgagg tccgaggcgg tccggaacgg cgtcgaggtc
     2461 gggcacgcac ccgcgcagat gccgcaacca acacagagcg ccgggttgac gatggcctgc
     2521 cgttcgaaag gacgtccatc ggttcggctg cccatcgtga tcgcattgta cgggcagtcg
     2581 ttgaaacaac gtgtgcagcc gttgcaattg gcaagatcca ccaccgcggc tgccggcttg
     2641 cgcagcggcg gcagccacgg catggcaatc aggatcagga gaagagcgcc cgaggcaccc
     2701 catgtcaccg gcccgggcca gcggtcgagc agcggataga gcggcagata gaaccagtct
     2761 agccccactg tcgcgggaac cttcgcgaga tcggccggcc cctggctcgt tgccggatgg
     2821 acgagcgaga gagtcagcaa ggcgacgaga acgccgatgg caagcccgcg tggtggattg
     2881 atgcgaggct tcgtcacgcg ctgcaaatgc acccacaaga tgatgagcgc tatgagcggc
     2941 actgcgatgt gcatgaagac catgagcgtg aagaagcggc tctccagcgt atcgggcgcg
     3001 aagaaattgc gcgcgatcgg ctcgccgaaa ataggcaatt tgtcgagcca ctcggtcgtc
     3061 accagagcga cgtactgcgc gagcgtatcc cacaccatcc agtagcccgt aatgcccgcc
     3121 acgaaaacga gcacgagcac gggcacgccg gtgacccagc tgaaccagcg cacgccgcga
     3181 taccggtcga gcccgaactc ccgtaagaga tgcgcgacca tgacgacgac gagcccgtcg
     3241 gacgcatagc ggtgcaggct gcgcatgacg ccggccgcgt accactggtc taccgtcatg
     3301 tactcgacgg agtcgaatgc gttgtgaaca gtcgtttcga aaaagatgaa aaggtagatt
     3361 ccgctgaccg tgatgatcca atagaagaaa aatccgagcg cgccgagatt gagcagcgga
     3421 ttccactcgg gtgggaagat tttgctgaga ccggcttcgg cgatgtcgaa accacggcgt
     3481 atgagggcgc gaaggacctt catcaggggc tcactccgtg tccggatgag cgccgccatt
     3541 cccggatcag cgccacgatg atcaggctga agctcgccaa cccgataccg atgctgatga
     3601 acagcgaata ggagaaataa tagcggccgg acttcggatc gtagatcgtg cagaaccagc
     3661 ggatacggtc gacgaggcct tcgagcgatg cgagcggttt gtttcggcca agcacgaccg
     3721 ctttcaacgg atcgacgatc accggcggct cgaaaacggc tccatagatc tgctgcctga
     3781 cacggccgtc tgcaccgatg aggctgacct gggccagatg gtcgaagcca cccgcgcggc
     3841 tgtaaatccc gaatccgacc gctttcgcaa gcgcatcaag gctttcctcg tccgaggcaa
     3901 ggaatttcca gttgggcaga tcgatgccgc gcgtgcgtgc gaacaggcga agccgggagg
     3961 gcgtatcatt ccgcgtatcg aagccgatcg tgacgacgtt gaagctgtcc tcgcccaaga
     4021 cttggcgcgc ggttttaacg gccggataaa ggctttcgat cagggttggg cagacgtcgg
     4081 cgcatccggt atagatcagg ctgacgagca gtggtttccc tcgataatcg gacagcttga
     4141 ccgtgcggcc ctcagtgtcg gtgaatgcga gatcgggtaa ttcttggccg agcgctgcct
     4201 gcgacagcgc aatggcattt tcggcattgc tggctgcagc tggcgaaacc gttttcaagg
     4261 aagcaaacaa gcttagagcc ataattagag gccagcccgg cctgctcata accttctcct
     4321 ctacgcgtcg acccggcgct cttttctttt tgcggcgttc gcggttagct ggatcccggg
     4381 cacgaggccc gggatgacaa acacgagacc taccgcccta ccccatcggc cagagctgcg
     4441 agagatactt ccagttgacg aaatagtaga gcacgaaggc agtgaaaaag acggtcacga
     4501 gaatgtacgt gcccggcaac ttccaggtcc ccgcattacc ataggtcgaa accgcatcgc
     4561 tcagctggcg ctgcggcata ggcccgagcg actcgatgat gttggacttg tccacgggct
     4621 tgccccaaag gacgctcgcc accacgatga ccacgaacaa tacgcctccg gtggcggcaa
     4681 tgatcgccga tatcccgttg agcgtcagca tgaggaacgc cgccgccgga taatcgaacg
     4741 acagtatcgc gtccgtgaat ccgacgtccc agtgccggcg cgcgacgccc agcgtgcctg
     4801 cgcccatcat gaacagggaa atgccggcag caccgattcc gaatagatac ggctgccatt
     4861 tggcgagttt cggccagatc acctggcgct gaaaaatcag cggcagaagc agataggtga
     4921 ccgccatgaa ggcgagcgtc gtgcccgtca cgaccgtggc gtggaaatgc cctggcacat
     4981 aaagcgtatt gtgcagcatg atattgatct gctcggtgcc gaggaccacg ccggaaatgc
     5041 cgccgaggaa gccgaacatc acgagcgaca ggaacatccc cgcgaaagcg ggattgcccc
     5101 acggcgcctt ccgcaaccat tcgaatgcgc cgcgcgtata gccgttacgc cgctgcgccg
     5161 cttccagggc gccgggcacg gtcatgccgt gaatcatgct gccgagcacc gccagataca
     5221 tgaaatagga cgtgttgaac acacgccact cggcgctgac gccgggctcg accagcaggt
     5281 ggtgggccga ggcgagctgc agaaacagga tatagaggaa aaatgcgccg cggctcacct
     5341 tttccgaaag cggcttggca ccgagcagga tcgccatgat tgcgtaccag atcgcgacgt
     5401 gcgccgacac gttcacctgc tgcgagccgt gtcccatggc ccaccagacc agcttgtaca
     5461 tcaccgtatt gatctcggag atatagccga tcgaccagag ccaggtcggc accaggacga
     5521 tggcgcccga ggccagtgtg aagatggcga tgatgcaggc cgtcagagca ccgaacgtca
     5581 cgagcgggat cgagccctcg taagttttct cctctttcgc aatgaccagc gtgccgagaa
     5641 aaacgaagca gccgagcaaa gcaccgacag cgaacaaaat caggccaaga tagaaattgg
     5701 gctccgcctg catcggcgga tagcttgtga acatgacgct cgattggccc tgcagcaccg
     5761 tataattggt cagcaggcct ccacccatca taagggcgaa ggcgatccaa gccactttcg
     5821 gcgctgccag ccggcagttg agcaggactg ccgaggcgaa ataaagcacc gccatttcga
     5881 agaacacgat ccagagtaaa agcacgtcga gaccgtgcgc ggtaagcacc atgtagaacg
     5941 catcagccgg cagcagatgc accgcctgcc agcgtgtcag ggccaccatg agacccagaa
     6001 gtccgcccac ggcaagccaa acgaccgcag cgaccgcatt cgccttgatc agccgctcgg
     6061 cagcaagatc gattttcagc ccgctggccg gacaggtgcg gaacctggcc gccacgccgc
     6121 cgatcacacc ggtttcagca gttgcgattg ccatgacctg tccccctatt gcggcgcgcc
     6181 cctggctgcc gtccccggct ccacaacgtg aatgcggccg accatcgtat ggtggccgag
     6241 gccgcaaaat tcattgcaaa cgatgctgaa tgtccccgcc tcggtggggg tcatggtgac
     6301 cacgtgctcg agctcgggat ggacctggat gttgatgttg accggctgca gcgaaaatcc
     6361 gtgctgccag tcgagtgagg agaggtgcag gcgatagctt tggccctttt cgagctcgag
     6421 caccggccac cactcccaca ggcgggccag catataaaca tccccaccgg ccggcgggcg
     6481 cacgacggga atgcccgtct ccccttcctc gcggactttg aacttctcag cgaattcgtc
     6541 gacgcgcttt tcgtaagcag cagggtcgat gcggtaggcc tgggtggata agttctgtcg
     6601 gccctcgatg tgccagtaga ccatcatgcc gaacatgagg aggccccaaa ggaaggcgat
     6661 tgcgatccag atgagctcgt ccttttcgac cggctcattc caccagacac gctgctcggg
     6721 aggatgaatg gccatgctct catcctccta tttggcgatc ggaatgtgcg tgagctcgat
     6781 gagcccccag agaatgtaga agacggtggg gatcgtcacc ccgagaaaga ggagcagaaa
     6841 cggattgtcg agagcccgtt gcattgcagg gattctctct ggctcagtgg gcggccgttc
     6901 gggcgcatgc tggccgatat tcgccatggc ggactccttg ccgaaaatat cagcttgatg
     6961 tctaatcgcg tcggtcgatc gagccttgac ctcggttaag caagatcagc gatccttatt
     7021 ctttcattcg gatttctgtt cttccaaact cagacgcaag ccgcggatct aggtccggaa
     7081 ttctggtatc gcgaccaatc ttctgagccg gctcgttaac ctcctgaaaa tccgccccgc
     7141 cgcacttgat ctcggttaag tccacccccg cccctgcgcg cctattcagg acatcgaatc
     7201 cagctcaacc tccgaaacta tgtttcttca gttgcgagat ctgtcgcagc acctcgtccc
     7261 tgttgtaggc gtgctcgcgt tcatcgtcgc ggtcacttcc gtcatcctga tctggcaggc
     7321 ccggcggcag gccgtctacg aaggaaagat caaggcggag ctggtttcac gggttgccag
     7381 cgagcagaca ttacgcgcgt ttcagacgac cgagctcgct ctccagagac tggggtatct
     7441 tctcgagcac ggacctccat tgcccgagaa cgacgaaggg ttcgcaagag agatgcaggc
     7501 ggctattcgc gacctgcccc acatccgcgc tatccacgtc gtcgcgccgg acggcttcat
     7561 cattcatgct acgactttcc cacactcgac ccgaattcgc gtgtccgaca gggactattt
     7621 cgttgcgcaa ctgaaaagca ccccggaagg caggggaacg ggagtttata taggacagcc
     7681 gatcaggagc cgtgggtggg agggatggtt cattccggtg agccgccgta tcaatcgacg
     7741 cgacaccggt ttcagcggcg tcatcgtggc tgcgatcgag ccgaagtact ttgaagacct
     7801 gttccgtgac ttgaacctcg agagcggcga cgcactcgcg cttttcaaca cggatgggaa
     7861 tttgatcgcc aggtttccta agtcgcccgg cctgatcggc agatcctggt cgcaattgag
     7921 actacccagc ggcggcaacc agctttcccc gacgcacgcc ggtcttcgcc tcggccagag
     7981 tctcgagaac aagccggtcg tgatcagtta tcgccgtctt gcgacctatc cggtcatcgc
     8041 ggccgtgctc ctcggccagc gagagctatt ggcgcactgg cggcaggtcg cgttggaatg
     8101 gggcgtttca agcgtcgcgc tgatcgtgct catgctggcg ttcgccgttg tgttgcagcg
     8161 ccgcaggcag gagcgtcaga ccgctgaagc ccgcgccctc gccatccaaa ggttcgagac
     8221 ggtcggccat acgacgagcg ggattgcgca cgatttcaac aacgtcctgg ccgtcgttgc
     8281 tgcaagcctc aggcttctac ggaacggcaa gccgccggac caggtactgc cggctgcgga
     8341 gcaggcgctc gagcgtgggc agcgtttgac ctcgcagctc ctcgggttcg ccagacgcca
     8401 cagcctggag atcaagccgc atgacatcga tcagctcgtg gttgccctgc agccgatgct
     8461 gaagcaggcg gccggtccga aatgcacagt cagctatcgg ctgaacagct ctggcacgcg
     8521 atgccacgcc gatcaggcgc agttcgatgc tgcgatgctc aacctcgtcg tgaatgcggc
     8581 ccaggcgatg gaccggggct ccatcgaaat catcaccgag ccggtgatgc tcgatcgcag
     8641 atcgaagaca gccctcaagc ccggccgcta tgtttccgtg aaggtcaagg acgatgggtg
     8701 cggtatcccg gccgatgccc ttggccgggt cttcgaggcc ttttatacga ccaagaaaga
     8761 gtcgggcaca ggtctcgggc tggcacaggt ctacactttc atgcactatg ttcatggcga
     8821 tgtgagaatt gaaagccagg tcggcgtggg caccaccgtg gaactgctgt tcccgtgcgc
     8881 gtgaaatgga tcagcacgtt cgagacattc tgcaaagacc atcgatggga gttctagcag
     8941 cgagttccgg cggcgtcgca gcattgcgca aggaggccga aacagagacc tcaatggatc
     9001 ttctttcgac tcgcgccagt tgcaacagct cctcgatcag cctcgtacga tcctggccac
     9061 caagtcgatc aagctttgcc ctcagccgtt tgatggcccc ggtttcagtc ttggggctgc
     9121 aggcgtgatt gtgctggaat gcacgtgcaa ttgcgattag gctcgaaccc gggaggaagc
     9181 gagcagcatt caagatgaga cctgatgaaa atcgcgcggg agcggaacac ccgttgctca
     9241 tcccggctgt gcgcgcgagt gcttcgttgc tccatttcac catcgacggc actccggcga
     9301 cgtgctcggc cggacggacc gtcctcgcgg caatcctgct gtcgcgaacg agtctgaata
     9361 cgaacgaagc aagcggcgag ccacgtgccg gcttctgtct catgggcgca tgccaggact
     9421 gctgggtgtg gctcgaggat gggacccggc tcagggcgtg caccacgccc atcgctgagg
     9481 gaatggcgat tctgaccgaa ccgccgacca ggcggcagcg aaatggctga cgtggtaata
     9541 gtcggcgcag gacctgcggg catagctgcg gccgacgtac tggtctcgaa cgggatctcc
     9601 cccacagtca ttgacgaagg tgcaaggcct ggcggacagg cttatcggac cagcg
//
LOCUS       DASVDU010000226        19599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1190986, whole genome shotgun sequence.
ACCESSION   DASVDU010000226 DASVDU010000000
VERSION     DASVDU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19599
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190986"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VF226_14345"
     CDS             complement(<1..502)
                     /locus_tag="VF226_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7075215.1"
                     /translation="MTASVQDGSAPPIAARVRSVSHVFKKVRALDDVTLDFPSGRMIG
                     LIGPDGVGKSTLLSLIAGARKVQQGDVEALGADMRDARQRREVGPRIAYMPQGLGKNL
                     YPTLSVFENVDFFGRLFGHAREERERRIRELLKATGLDPFLDRPAGKLSGGMKQKLGL
                     CCALIHD"
     gene            complement(499..1530)
                     /locus_tag="VF226_14350"
     CDS             complement(499..1530)
                     /locus_tag="VF226_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7075216.1"
                     /translation="MVAAVAAGWYLTQRLSEPPLPDWIAWGNGRIEAVAIDISARTGG
                     RVREIVVQEGQTVARGEVLAHMDTQQLEAQLREARAQLQRGKIARDVAESGVIQAEAE
                     HSATLAVVAQRRTELESAARRLDRSEQLARTNAISQQTLDDDRARVAGAQAALDAARA
                     QAASAEAAIATARASIVDAEASIEATEAAIERIQAEIDDSTLRAPRDGRVQYLVAQPG
                     EVVAGGGRVLNLIDLNDVFMTFFLPTVEAGRVALGSEARIVLDTAPGVVVPAQVSFIS
                     DVAQFTPRTVETREERISLMFRVRVRVAPELLRKYANYVKPGLPGMAYVKLDPDAQWP
                     EALSTGLLE"
     gene            1932..4742
                     /locus_tag="VF226_14355"
     CDS             1932..4742
                     /locus_tag="VF226_14355"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX7075217.1"
                     /translation="MAHMPGDGEPHLRTKEDVLAALGTDAHQGLTEAEATARLECFGP
                     NQLDAVRPVPAWRKFLAQFTEVLVLLLIVAGVISAALWFFEGGSALPFEAIAIFVIVL
                     LNGIMGYIQQARAEKAVQALRTMSAPHATVIRNGERRRIPSIQLVPGDIILLEEGDAV
                     PADARLIETTALQTTEAALTGESMPVSKHPSLVATGAVLGDRHNMVFSGTAVAYGRGK
                     GVITATGMQTEMGRIAGMLEKAPEERTPLQDELDRVGRTLGKIVVAIAAIMIGTILLV
                     DDVQGFSALFDVLILGVALAVAAVPEGLPAIVTAVLSIGVQRMAKRKAIVRHLAAVET
                     LGSADVIASDKTGTLTRNEMTVRRIVTASGQVRLTGRDHPEMEPVGGADSACIQAELK
                     RALIAAERANNAVLQQNDGEWSVQGDPTEAALIAAAWKAGLDCAALDHRFKRVAEIPF
                     SSERKLMSTIHADAGRESVLRAFTKGAPDVLLARCSHELVGEEARPLTAERRAEIMAA
                     NEALASEALRTLGVAFRSIPAQEVNEAPSYVDVENDLVFLGLIGMMDPPREEARQAVA
                     EAKAAGIRPIMITGDHPRTAAVIAAELGITDNLRAITGAELETMPVEQFDQTIRDVSV
                     YARVDPAHKLRIVDALQRQGATVAMTGDGINDAPALKAADIGIAMGITGTDVSKEAAD
                     IVLADDNFATIVAAVEEGRAIFSNIRKFLRYLLSSNIGEVMTMFFGVLLADVIGLVGP
                     DGGLVLPLLATQILWINLVTDGAPALALGVDPASASIMRRPPRPRGEGVIPRRMWAGI
                     FLVGTVMAAGTLAVIDGSLPGGLIEGNGSVAYAQTMAFTTLTLFQLFNVFNARSDELS
                     AFSGIIANRWLWAAVVLSLMLHAAVVYVPFLQQAFSTVSLGAADWLVCTAVASSVLWV
                     RELAKLAVGRISRSRFDRTAAA"
     gene            4770..5639
                     /locus_tag="VF226_14360"
     CDS             4770..5639
                     /locus_tag="VF226_14360"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7075218.1"
                     /translation="MRLDRPKSPRRILLATDLDPRSDRALDRAALLAGEFSAELFVAH
                     VLEDIPDGEDEIPSWRRPPSLVEVARRQLLRQVGAVAQKATILIEQGEPAKAILAAAE
                     REGCDLIVTGVAKEELFGRSSLGATVDGILRGTGVPVLVVKSRASNPYRQIVVATDFS
                     RYSRHALDTAAAFFPDRPLTLFHAYDAPMSLLSSDPDAYRQEWEEAARKECANFLRDS
                     SHDADQYIAFGAPDVVMHDYVLEKGVDLVVLGTRGRSGIGEFFLGSVAKSIVLAVPCD
                     ALLVRHREELEEA"
     gene            complement(5664..5993)
                     /locus_tag="VF226_14365"
     CDS             complement(5664..5993)
                     /locus_tag="VF226_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX7075219.1"
                     /translation="MLEPYLIVSIVRRGWGDTILEATMNAGAHGGTVLVGRGIGRNEQ
                     QRVFGIQIEPEKEVVLTVVPADLKDAVLRAVEEAAELNAPGKGLAFMLPLEKVVGVVH
                     LVQESPE"
     gene            complement(5998..6729)
                     /locus_tag="VF226_14370"
     CDS             complement(5998..6729)
                     /locus_tag="VF226_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX7075220.1"
                     /translation="MELNGTFLDHIAETAFSIAVAIVPLAALFIAFQIFMLRLPRREV
                     RDILLGTVLAAAGLFLFLLGVGIGFLPFGRAIGEAVGAVQNLWLLIGIGLFLGFVTTW
                     GEPAVRILAEQVDEASQGAIPGILVLYAVCLGVAVWVMLGLLRIKYGIPILYLLLPGY
                     IIAVLTIWLSSRDFVAIAIDAGGVATGPLANTFLLALALGASSAMDDQSPVTHGLGLV
                     ALIAVAPIISVTILGLLIGRKGAKE"
     gene            complement(6720..7415)
                     /locus_tag="VF226_14375"
     CDS             complement(6720..7415)
                     /locus_tag="VF226_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEX7075221.1"
                     /translation="MASKTTEKTIEVLQAVAPLVGVVCLLQVTLVHAPLGLFLQFLLG
                     AALAGAGMLLLLLGVDLGILPMGRFIGAELPKKSSAALIVVVAFSLGFATTVAEPDVL
                     VLAGQADKASQGSIPGMAVLYVMAVGVAILTAIASLRILFGWSIKTLVLAAYVTMLGL
                     ALAAPEEFVPLAFDAGSVTTGVLTAPVLISIAMGLSSVLAGRSAVSDGFGISGLASAG
                     AILSVLLMGFLWS"
     gene            7625..8347
                     /locus_tag="VF226_14380"
     CDS             7625..8347
                     /locus_tag="VF226_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7075222.1"
                     /translation="MQQQDLSKCGVPAACIFCGARQRGMCGALEPRQLAELSKYSVRK
                     NASSGKELYSAAEKANGFSNILSGVVKLSKTLSDGRQQIVSLQFPPDFLGRPFRAEST
                     VTAEAVTEVVLCSLPQTAVERMIAESPSLEHHLYQQSLRELDEARELMVTLGRKTAEE
                     KVASFLMMIARHLDPAGHELKSAAFDLPLTRADIADYLGLTIETVSRQLTKLRSGNII
                     QIINSHHVIVPNMARLAARAGN"
     gene            complement(8301..8852)
                     /locus_tag="VF226_14385"
     CDS             complement(8301..8852)
                     /locus_tag="VF226_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7075223.1"
                     /translation="MTRPPYIAPKKESCPFASRCKAQRCLVCKGSNAHFEIKLELCSA
                     LEAIADGLPSLVDRNTCLRVAEALLPAFQMAHAFEEEAVYPTFSAEAFCSEERQKTLA
                     RLRVEHVEDEALAEEITERLLPIGYGSGVENPEALGFMLRTFFRSVRRHVAFEREYIL
                     PVVGGWPLPNFLHGRPGGPYSVR"
     gene            complement(8884..9153)
                     /locus_tag="VF226_14390"
     CDS             complement(8884..9153)
                     /locus_tag="VF226_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075224.1"
                     /translation="MHGTAAGAQLALAAKGIWIQRAGEQMIGTAPEGLKQRLSAFLPA
                     EEAARLLTRIGDFFQFPGAWASVRVLVARINQHLARGEVIRSRIG"
     gene            9448..10884
                     /locus_tag="VF226_14395"
     CDS             9448..10884
                     /locus_tag="VF226_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEX7075225.1"
                     /translation="MTTASGELVERKRFRFPTAFTILFGLIVAVAVLTWIVPAGQYER
                     TFSESLGREVPIAGTYAETEPNPQGIFDVIMAPIAGIYDPATNEANAIDVALFVMVLG
                     GFIGVIAATGAITAGITRAMAALRGREKWMIPVLMGLFAFGGTTYGMAEETLAFYVLL
                     IPIMIAAGYDAVTAVAIILLGAGVGVLGSTVNAFATVIASDAAGIPFTTGMVLRLVLL
                     GLCWLACVIYVMRYAERVRADPSRSIVYDRKEENERHFLSSLKANDRPEFTTLHKVVL
                     AVFGLTFVVMIWGVLFGGWWMGEMTGLFLASAILIGLIARPGEQEFVEAFLSGVRDLV
                     GVALIIGLARGIVVIMDAGRITDTILHWAEVSVAGLSEVLFVNVIYWIEVLLSFFVPS
                     SSGLAVLSMPIMAPVADFAGVGRELVVTAYQSANGIVNLINPTFAVVMGALAIGRVPY
                     ERWLAFMWPLLLILTVIIMACLSVAVLI"
     gene            10897..12123
                     /gene="arcA"
                     /locus_tag="VF226_14400"
     CDS             10897..12123
                     /gene="arcA"
                     /locus_tag="VF226_14400"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEX7075226.1"
                     /translation="MTQTYGVHSEVGKLRRVLVHRPGTALARLTPRNREELLFDDVIW
                     VKQARVEHMTFVDAMRYRGVEVVFLRELLAETMRIAEARGWLLDRRVNDNTVGVGLAD
                     DLHAYLTEIDADELSNILMGGMSRAELPIKANSVVASMLAPEEFILPPLPNHLFTRDT
                     TCWIYEGVTLNPMRWNARQLETVNIAAIYKFHPDFRDAGFPVWWGDPDRNYGRATAEG
                     GDVMPIGNRTVLIGMGERTTPHAVGQIARALFAQGGAERVIACRLPAERASMHLDTVF
                     TFCDRDLVTVFAGVVKDIQAFSLRPGDKEWQIDVRAEERPLTEVVAEALGLKELRVVE
                     TGGDAYVREREQWDDGNNVVAIEPGVVIGYDRNTYTNTLLRKAGVEVITVPGAELGRG
                     RGGGHCMTCPLIRDPA"
     gene            12136..13137
                     /gene="argF"
                     /locus_tag="VF226_14405"
     CDS             12136..13137
                     /gene="argF"
                     /locus_tag="VF226_14405"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7075227.1"
                     /translation="MPVNLLGHNFLKLLDFTPDEIRFLLRLAASLKQAKYGGYERQTL
                     TKKNIALIFEKDSTRTRTGFEVAAYDQGAHVTYLGPTGTQIGKKESIKDTARVLGRIY
                     DAIEYRGFSQETAEELARHAGVPVYNGLTDEFHPTQILADFLTMRECTRKHLSELKFC
                     FLGDAGNNMGNSLLSGAALIGMDVRLAAPKSCWPDAALVEQCRSIAEKTGARITLTED
                     PMEAARGCDFVYTDVWVSMGEPDDVWAERIKLLTPYRVTAEIMAATGNPHAKFMHCLP
                     SFHNRETEIGERIFNQYGIDCMEVTEEVFESETSVVFDQAENRMHTIKAVLVATIGG"
     gene            13139..14053
                     /gene="arcC"
                     /locus_tag="VF226_14410"
     CDS             13139..14053
                     /gene="arcC"
                     /locus_tag="VF226_14410"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012482187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX7075228.1"
                     /translation="MRVVVALGGNALLKRGEPMTAEVQRANVRVAAEALADLARDHEI
                     VVAHGNGPQVGLLALQAAAYKGVEPYPLDIIGAESVGMIGYLVEQEMVNAMPPGARCA
                     TLLTQIEVDPDDPAFKRPRKPIGPLYDKVEAHRLRYQKKWPMCEESEGNWRRVVPSPE
                     PRRIVEISVIRLLVEAGVTVICAGGGGIPVIRNRNGELEGVEAVIDKDHAAGLLAREI
                     DADAFLMLTDVEAVYADWQTPAPRPIRATTPEELSGMAFAEGSMGPKVEAACVFAATR
                     GRMAGIGRLQDARAILEGRAGTLVTTAN"
     gene            complement(14101..14856)
                     /locus_tag="VF226_14415"
     CDS             complement(14101..14856)
                     /locus_tag="VF226_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075229.1"
                     /translation="MANEFEGKVALVTGAASGIGAECARQLAAAGAAVVVADLDERSA
                     KKIVEEIERDGGKAAAFRIDVAVAEEVDAMVVFAKSTFGELHLAVNNAGISGASELTG
                     DYPPDGWRKVIDINLSSVFYCMRYEIPAMLEAGGGAIVNMSSILGTNGFARSSAYVAA
                     KHGIVGLTKTAAIEYAAKGIRINAVGPGFIDTPLLSKNLKPDELKQIVGLHPIGRLGR
                     AEEVAALTCFLLSDRASFITGSYHLVDGGYSAP"
     gene            15439..16623
                     /locus_tag="VF226_14420"
     CDS             15439..16623
                     /locus_tag="VF226_14420"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7075230.1"
                     /translation="MVLVGSSVTCIAAGLCWSATYLLAGAVIAAAVPAIYAVLSLAST
                     ALFALTRSYRIYVALQLTLIFFLPLCMSVILGDQASAVVIWSALAPFGALLFASRRTA
                     LVWFALLLLALASTVVPLPEIERPLPHGFVTAMFVLNIGALLALLFAMTAYFIRQMHL
                     LQDRSEMLLHSILPKHIAEHLKGSPSRTAKQHDEASILFADIVGFTALSSQLSPDELV
                     TMLDEVFVEFDLLVDQLGLEKIKTIGDCYMVASGIPLPRADHAHVLAQLALAMQRTVA
                     NRLFCGQRLAFRIGMNSGPVVAGVIGRKKFIYDLWGEAVNIASRVEGGGTGGAIQISA
                     ATHALLKGDFVCVPAGKVRITGEHEVEVYHLIGEKRSLPDFIGESSKAGRQQFADGRL
                     LP"
     gene            16620..18785
                     /locus_tag="VF226_14425"
     CDS             16620..18785
                     /locus_tag="VF226_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7075231.1"
                     /translation="MSSVAFVKSLLSGLIIGLTAIVFSISFTAIIYTGELAPFLSRGI
                     GLTLAGAAVMAVAGAFTLSYRGIIVQPQDVTALILSLAAASIAGNWQGAASDDLFATI
                     AALVAVATATTGVAMVVFGRLRLGHIVRFIPYPVLGGFLAATGYLLVTGAIGMTIGDT
                     VSVRSANVLFDAGNPQKWAPWIVAAVLFYVAVKRVRHGLVLPACFVAAVLCFYGALKL
                     FGIDQATAQTRGLLLGPFGEATLYASLDPGLLVRARWDVVAAQLPSILAVIGMATIGS
                     LLNVSGLELATGEDIDPNRELHGVGVANLAASAVGGMVGYHLLSQTLFAKALGVTGRA
                     AGISVALVTLAVLFLGTTYLSVLPIGVFAAVIAFLGIDLLVTWLWVERRRLQLRDFFV
                     IVLILAVAATIGFLEAIAIGILAASTLFIVAYSRVDVVRLKTTGARKRSGVERGQRDL
                     AILSDRGDQVAIYELSGYLFFGTAHRLVSEISEDTVAQSKEFVLVDFKRVQGIDASAA
                     FALRRLVQLCAAGSVTLIFCGLSDRLSQTLDRAGIPSEPSPPLLFKHLDDALQIVEEK
                     LLLDNAGREGIESDTGLLDELRRIARPLDPLGLFEVYDIGKGEVVIEQGSEADDILLL
                     VSGVLRVEYLRADGSKIPVAKILPGAVVGEIGFYAGERRTAQVIAEQDCRLMKISKRT
                     LADLEESQPGLVSNVHRIAATNLALRLTRAMALLRDADV"
     gene            complement(18793..19179)
                     /locus_tag="VF226_14430"
     CDS             complement(18793..19179)
                     /locus_tag="VF226_14430"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEX7075232.1"
                     /translation="MSSTLAPLWQMAGLLVVHWIGDFVLQSSWMAENKSDRLDALIAH
                     VITYSITLAVGAGVIFGIARPGALVSFVIANALLHMAVDFTTSRIAARFWRSGQWRAF
                     FIVVGADQLLHQICLALTLSMLIKQT"
BASE COUNT         3773 a         6185 c         5988 g         3653 t
ORIGIN      
        1 gatcgtggat cagcgcgcag cacagaccga gtttctgctt catgccgccc gagagctttc
       61 cggccgggcg gtcgaggaac gggtcgagac cggtcgcctt cagcaattcg cggatgcgcc
      121 gctcgcgctc ctcgcgtgca tggccaaaca gccggccgaa gaaatcgaca ttctcgaaca
      181 ccgagagcgt cgggtagagg ttcttgccaa gcccctgcgg catataggcg atacgcgggc
      241 ccacttcgcg gcgctggcgc gcatcgcgca tgtcggcacc cagcgcttcg acatcgccct
      301 gctgaacctt gcgcgcgcca gcgatcagcg acagcagcgt cgacttgccc acgccatcgg
      361 ggccgatgag gccgatcatc cgcccggacg gaaagtcgag cgtcacatca tcgagcgcgc
      421 gtaccttctt gaagacatgg ctgacggagc gcacgcgcgc ggcgatcgga ggggcactgc
      481 catcctgcac tgatgcggtc attccaagag accggtgctg agcgcctcgg gccattgcgc
      541 atccgggtcg agcttcacat aggccatgcc gggcaggccc ggtttgacgt agttggcata
      601 tttccgaagc aattccggcg ccacgcgcac gcggacgcgg aacatcaggc tgatccgctc
      661 ctcccgcgtt tcgaccgtgc gcggcgtgaa ctgggcgaca tcagagatga aggacacctg
      721 ggctggcacc accacgccgg gcgccgtgtc gagcacgatg cgcgcctccg agccgagcgc
      781 cacgcggcct gcctcgacgg tcggcaggaa aaaggtcatg aagacgtcgt tgagatcgat
      841 gaggttcagg acgcggcctc cgcctgcgac cacctcgccc ggctgcgcca cgagatattg
      901 cacgcgcccg tcgcgcgggg cgcgcagcgt gctgtcgtcg atctcggcct ggatgcgctc
      961 gatggctgcc tctgtcgcct caatggatgc ttccgcatcg acaatgcttg cccgggccgt
     1021 ggcaattgcc gcttcggcgg atgccgcctg agcccgcgca gcatcaagag cggcttgtgc
     1081 acccgccaca cgggcgcggt cgtcatccag cgtctgctgc gaaatggcgt tggtgcgggc
     1141 aagctgctcc gaacggtcca gccggcgcgc tgccgattca agttccgtcc ggcgctgcgc
     1201 gaccacggcc agggtcgcgg aatgttcggc ttcggcctgt atgacgccgc tttcagcgac
     1261 atcgcgcgcg atctttccgc gctgcagctg ggcccgcgcc tcacgaagct gagcttcgag
     1321 ctgctgagta tccatatggg caaggacctc gccgcgcgcg accgtctgcc cctcctgcac
     1381 aacgatttcg cggacgcgcc ctcccgttcg cgcggaaatg tcgatcgcca ccgcctcgat
     1441 gcgcccgttg ccccaggcga tccagtccgg cagcgggggt tcgctcagcc gctgcgtgag
     1501 gtaccagccg gccgcgactg ccgcgacaat ggcggcaatc cacagccaac ggcgcgacag
     1561 gatgttgctc gcccgcttaa ccacagcccg ctacctccgg agcatcccgc gagcatgacc
     1621 gaaagatccc ggtggtgtga gcgcgtctgt ctgcctcaag tgtttcagga tcacgcgctg
     1681 ccaaccaggc tccggcgaga tacttgcatg tttcgcgata cccgtaaatt cagccgtctg
     1741 ctcggccccg gcgaacccaa tacaggctag aacgttgtcc cgtgacaaaa tgtgccgagt
     1801 acgggaatgc ttaaaagcag cacctcacta cggcatggga agggaaccgc cgagcggcgc
     1861 atcccactcc gggagcctat gggatcgtga gagcgtcgac cgcagaacca ccggaagcca
     1921 gcgtgcagga tatggctcat atgcctggcg acggcgagcc gcatctgcga accaaagagg
     1981 atgtgctggc agcgctcggc acggatgccc atcagggcct gacagaggcc gaagccacgg
     2041 cgcggcttga atgtttcgga cccaatcagc tcgatgccgt gcggccggtt ccggcctggc
     2101 ggaagtttct ggcccagttc accgaagtgc tggttctcct gctcatcgtg gcaggcgtca
     2161 tttccgccgc actctggttc ttcgagggcg gctccgcatt gcccttcgag gcgatcgcca
     2221 tcttcgtcat cgtgcttctc aacgggatca tgggctacat ccagcaggcg cgtgcggaga
     2281 aggccgttca ggcgctccgg accatgtcgg cgccacatgc cacggtgatc cgcaacgggg
     2341 aaaggcgacg cattccgtcc atacagctcg tgccgggaga catcatcctc ctcgaggaag
     2401 gcgatgcggt tccggcggat gcgcggctga tcgaaaccac cgcgctgcag acgacagaag
     2461 ccgcgctcac cggcgagagc atgccggttt cgaaacaccc ctcgctcgtc gccaccggcg
     2521 cggtgctggg cgaccggcac aacatggtct tcagcggaac ggccgtggcc tatggacggg
     2581 gcaagggcgt gatcaccgcc accggcatgc agaccgaaat gggccgcatc gccggaatgc
     2641 tggagaaggc tccggaagag cgcaccccgc tgcaggacga gctcgaccgc gtgggccgca
     2701 cgctcggcaa gatcgtggtg gccatcgcag cgatcatgat cggcacgata ctcctggtgg
     2761 acgatgtcca gggcttctcg gctttgttcg acgtgctcat cctcggcgtg gcgctcgccg
     2821 tcgcggccgt gcccgaaggc ctgcccgcaa tcgtgacggc ggtactctcc atcggcgtcc
     2881 agcgcatggc aaaacgcaaa gccatcgtgc gtcacctcgc ggcggtggag acattgggct
     2941 ccgccgatgt cattgcctcc gacaagaccg gcacactcac cagaaacgag atgacggtgc
     3001 gcaggatcgt gaccgcgagc ggccaagtgc ggctcaccgg cagggaccat ccagaaatgg
     3061 agccggtggg aggcgcggat tcggcctgca tccaagccga gctgaagcgc gcgctgatcg
     3121 ccgccgagcg cgccaacaac gcggtgctgc agcaaaatga cggcgagtgg tcggtgcaag
     3181 gcgaccctac cgaggccgcg ttgatcgctg cagcctggaa agcaggcctc gactgtgcgg
     3241 cgctcgatca tcgtttcaag cgcgtcgctg aaatcccgtt ttcgtcggaa cgcaagctga
     3301 tgagcaccat ccacgccgat gctggacgtg aaagcgtgct gcgcgcattc acgaagggcg
     3361 cgccggacgt actcctggcg cggtgttcgc acgagctggt gggcgaggaa gcaagaccgc
     3421 tcacggcaga gcgccgggcc gaaatcatgg cagccaacga ggcattggcc agcgaggcgc
     3481 tgcgcacgct gggtgtcgcc ttccgctcca tccccgcgca ggaagtgaat gaagccccct
     3541 catacgtgga tgtcgaaaac gacctcgtct ttctcggtct gatcgggatg atggacccgc
     3601 cgcgcgagga agcgcggcag gcggtggcgg aggcaaaagc ggcgggcatc cgccccatca
     3661 tgatcacggg cgaccatccg aggaccgccg ccgtcatcgc cgccgagctc ggcatcacgg
     3721 acaatctgcg cgcaataacc ggtgctgagc tggagacgat gccggtcgag caattcgatc
     3781 agacgattag agacgtttcg gtctatgccc gtgtcgatcc agcacacaaa ttgcgcatcg
     3841 tcgatgcgct gcagcggcag ggagcgaccg tcgccatgac cggagacggc atcaacgacg
     3901 caccggccct caaggcggcc gatattggca tcgccatggg catcaccgga acggacgtgt
     3961 cgaaggaagc cgccgatatc gtgctcgccg acgacaattt cgcgaccatc gtcgccgctg
     4021 tcgaggaggg gcgcgcgatc ttctcgaaca tcaggaagtt tctgcgctac cttctttcct
     4081 cgaacatcgg ggaagtaatg acgatgtttt tcggcgtgct tcttgccgat gtgatcggcc
     4141 tggtggggcc cgacggcggg ctcgtccttc cgctgcttgc cacgcagatt ttgtggatca
     4201 acctcgtcac cgatggggca cccgccctcg cgctcggcgt ggaccccgca agcgccagca
     4261 tcatgagacg gccgccgcgg ccacgcggcg aaggcgtcat tccccgacgg atgtgggccg
     4321 gcatttttct tgtcggcacg gtcatggcgg ccggtacgct ggcggtaatc gacggctcgc
     4381 tccccggagg cctgatcgag ggcaatggaa gcgtcgcata cgcccagacc atggcgttta
     4441 cgacacttac ccttttccag cttttcaacg tgttcaacgc gcgctcggat gagctgagcg
     4501 cattctccgg gatcattgcc aaccgttggc tctgggccgc cgtggtcctg tccctcatgc
     4561 ttcatgccgc cgtcgtgtat gtacctttcc tgcagcaggc gttctccacg gtcagcctcg
     4621 gtgcggcgga ctggctggtc tgcacggcag tggcaagttc ggtgctgtgg gtgcgcgagc
     4681 tagccaagct tgccgtgggt cgcataagcc gcagccgttt cgatcgcact gcggccgcat
     4741 gagccgtatc aagggtgagg agacgaaaaa tgaggttgga ccgcccgaaa tctccgagga
     4801 gaattctcct tgccacagac ctggacccgc gttcggacag ggcgctcgac cgagcggcgc
     4861 ttctggccgg cgagttttcc gccgagctgt tcgtggcaca tgttctcgag gacattcctg
     4921 acggagaaga cgagataccg tcatggagac ggccgccgag ccttgtcgag gtcgcgcggc
     4981 ggcagcttct acgtcaggtc ggcgctgtgg ctcagaaggc caccatcctg atagagcagg
     5041 gcgaacccgc caaggcgatt ttggctgctg cagaacgcga gggatgcgac ctcatcgtaa
     5101 ccggtgtcgc gaaagaggag ctgttcgggc gctccagtct cggtgcaaca gtggatggaa
     5161 ttctgcgcgg cacgggcgtg ccggtgctcg tggtcaagag ccgcgcgtcg aatccgtata
     5221 ggcagatcgt cgtcgcaacc gacttctcaa gatactctcg gcatgcgctc gatacggccg
     5281 ccgcattctt tccggaccgc ccgctcaccc tgttccatgc ctatgatgcg ccgatgtccc
     5341 tgctgtcgtc cgaccccgat gcctaccgtc aggagtggga agaggccgcg agaaaggaat
     5401 gcgcaaactt ccttcgcgac tcgagccatg atgcggatca atacatcgcg ttcggcgcgc
     5461 cggatgtcgt gatgcacgac tacgtgctgg agaagggtgt tgatctcgtg gttctcggca
     5521 cccgcgggcg aagcgggatc ggcgagtttt tccttggcag cgtcgcgaaa tcaattgttc
     5581 tggccgtgcc ctgcgatgca ctgctggtgc gacaccggga ggagctggag gaagcataag
     5641 cggtatctca gtcaccccct ggcttattct ggagattcct gcacgaggtg caccacgccg
     5701 acgacctttt cgagcggaag catgaaggca agcccctttc caggggcatt caactcagcc
     5761 gcttcctcga cagcgcgcag gaccgcatcc ttcagatcgg cgggaacgac ggtcaggacg
     5821 acttcttttt ccggttcgat ctgaatgcca aagacccgtt gctgctcgtt gcgcccgatg
     5881 ccgcgcccga caagtacggt tccgccatgt gcgccggcat tcattgttgc ttccaggatc
     5941 gtgtcgcccc atcccctgcg gacaatgctg acaatcagat acggctccag catctcgtca
     6001 ctcctttgcg cctttccgcc cgatgagcag gccgagaatt gtgactgata tgatcggggc
     6061 aaccgcaatg agtgccacga gaccgaggcc atgcgtcacc ggactctgat cgtccatcgc
     6121 ggaggaggct cccagagcaa gcgcaagcag aaaggtgttc gcgagcggcc cggtcgccac
     6181 accacctgcg tcgattgcga tcgccacgaa atccctgctg ctcagccaga tagtgagaac
     6241 agcgatgatg tagccgggga gaagcaggta aagaatcgga atgccgtact taatgcgcag
     6301 aagcccgagc atgacccaga cggcaacgcc caggcatacg gcgtaaagca ccaggatacc
     6361 gggaatcgct ccctgagagg cttcatccac ttgctcagcc aggatacgga cggccggttc
     6421 tccccaggtg gttacaaatc ccaggaataa accgatgccg atcaacagcc acagattctg
     6481 tacagcgcct acggcttcgc cgatcgcgcg cccgaagggc aggaagccga tgcctacgcc
     6541 gagcaggaac aggaaaagtc cggccgctgc gagcaccgtt ccaagcagga tgtccctcac
     6601 ctcgcgccgc ggcagccgca gcatgaagat ctggaacgct ataaacagcg cggcaagcgg
     6661 aacaatcgcg acggcgatgc tgaacgccgt ttccgcaatg tgatccagga acgtaccgtt
     6721 cagctccaca aaaaccccat cagcaagaca gacaggatcg cgccggcgga tgcgaggccc
     6781 gatatgccga acccgtcgct tacggcggag cggccggcca gaaccgagct caggcccatg
     6841 gcaatcgata tcagcactgg cgccgtaaga acgcctgtgg tgacgctgcc ggcatcgaag
     6901 gcgagcggaa cgaattcttc cggtgctgcc agcgcaagac caagcatcgt cacataggct
     6961 gcaagcacaa gggtctttat cgaccagccg aagaggatgc gcaaactggc gattgcggta
     7021 aggatggcga cgccgacagc catgacgtaa agaactgcca ttcccgggat gctgccttgc
     7081 gaagccttgt ccgcctggcc ggcaagaacc agcacgtcag gctcggccac ggtggtggcg
     7141 aaacccaggg aaaaggcgac caccacaatc agcgcggcag agcttttctt cggaagctcg
     7201 gctccgataa accgccccat cggcagaatc ccaaggtcga ctccaagaag cagcagcaac
     7261 ataccagctc ccgccaacgc agcgcctaag agaaactgaa ggaacagccc gagcggggcg
     7321 tggacgaggg ttacctgcag caggcacacc acaccgacca ggggcgcaac tgcctgcaaa
     7381 acttcgatcg tcttttctgt cgtcttggaa gccatgacac gaaggatcag ctgcaaaccg
     7441 gaggcttgat cgtcaggtca ccatcccgca atttcaacgt tccagtcctt tttttgccgc
     7501 tatcgaactg caagctacgg cattgtgaga gaggagctag cggcaagcca cacggcaatt
     7561 ggtcgcaaga tttgctcgca agcccggcgt agcattgtgc ggcaaacgcc aaacgagtag
     7621 ccccatgcag cagcaagact tatccaagtg tggtgtcccc gctgcctgca tcttctgcgg
     7681 cgcacgacag aggggcatgt gcggcgcgct tgagccacgc cagctcgcgg agttgtcgaa
     7741 atactccgtg cgaaagaacg caagcagcgg aaaagaactt tattcggctg cggagaaagc
     7801 aaacggtttc tcaaacatcc tgtcgggggt ggtcaaactc agcaaaacct tgtcggacgg
     7861 ccgccagcag attgtcagcc ttcagttccc accggatttc cttggccgac ctttcagagc
     7921 tgagagtacc gttacggcgg aagcggtgac ggaggtcgtg ctttgctcgc tcccgcagac
     7981 cgcggtggaa agaatgatcg cagaatcgcc cagcctggaa catcacctct accagcagtc
     8041 cctacgagaa ctggacgaag cacgggaatt gatggtgaca ctggggcgta agacagcgga
     8101 ggaaaaagtt gcgagctttc ttatgatgat tgcccggcat ctcgaccccg cgggccatga
     8161 gctcaaatcc gcagcctttg acctcccgct cacacgcgca gacatcgccg attacctggg
     8221 cctgacgatc gagaccgtga gccgacagct taccaagctc agaagcggga atatcattca
     8281 aatcatcaac agccatcacg tcatcgtacc gaatatggcc cgcctggccg cccgtgcagg
     8341 aaattaggca acggccatcc cccgacaacc gggagaatgt actcgcgttc aaatgcaacg
     8401 tgacgccgca ccgacctgaa aaaggtgcgc aacatgaaac caagtgcctc gggattttcg
     8461 accccggatc cgtagcctat gggaagaagc ctttcggtta tttcctccgc cagcgcttcg
     8521 tcctcgacgt gctccacgcg caaacgcgcg agcgttttct gcctttcctc ggaacagaag
     8581 gcttccgcgc tgaaggtcgg atagacagcc tcttcttcaa aggcatgcgc catctggaag
     8641 gcaggaagaa gcgcctccgc cacccgcagg caagtatttc tgtcgacaag gctgggaagg
     8701 ccgtcggcga tcgcttcgag cgccgagcac agttccaact ttatttcaaa atgcgcattg
     8761 ctgcccttgc acaccaaaca tcgctgcgcc ttgcatctgc tcgcaaaggg gcaggattcc
     8821 tttttcgggg cgatgtatgg tggccgggtc aaggcgggct cgttgtttct cggggctttg
     8881 tgttcacccg atccgcgacc gaatgacctc gccccgtgca agatgctgat tgatccgggc
     8941 aactaagaca cgcaccgacg cccacgcgcc cggaaactgg aagaaatcgc cgatccgggt
     9001 gagcagacga gcggcttctt ctgccggcaa gaatgctgat aggcgctgtt tcaagccttc
     9061 gggcgccgtg ccgatcatct gttcgcctgc tctttgtatc caaatcccct ttgctgccag
     9121 ggcgagttgt gcaccggcag ccgtgccgtg cattgacacg gatcaaggaa gaaaacgctt
     9181 catccggaag gatcatgggg gacataagcc cgcgaaaagt tctatcagcg tcttgtatta
     9241 ttattttcct acatatgaaa gcaaaattgt tgacgcagat caaagaagcg ctccaaggac
     9301 ctgataatcg taaaaatcag caagtatgta ccgaggccgt gaaacagtaa cggcacggat
     9361 gctcaagtat tgacgcctta cgaaggtgcc ttggcccggc gggcgactcc ggcccccatg
     9421 ccgaacccat gtcaaaggta atgtgccatg accactgcat ctggagaact cgtcgagcgg
     9481 aagcgtttcc gttttccgac agccttcacc atcctgttcg ggctgatcgt cgccgtcgct
     9541 gtgctgactt ggatcgttcc ggcgggccaa tacgagcgca ccttcagcga gtctctgggg
     9601 cgtgaggttc cgatcgccgg cacttacgcc gaaacggagc cgaatccgca ggggattttc
     9661 gatgtcatca tggcgccgat cgccgggatc tacgatccgg cgacgaacga ggcgaacgca
     9721 atcgacgtcg ctctgttcgt gatggtgctc ggcgggttca tcggcgtcat agcggcaacg
     9781 ggagccatca cggccggcat aaccagggcc atggccgcgc tgcgcgggcg cgagaaatgg
     9841 atgataccgg tcctgatggg cctttttgcc ttcggcggca caacctacgg catggccgaa
     9901 gagacgcttg ccttctacgt gcttctgatc cccatcatga tcgcggccgg ctacgatgcc
     9961 gtaactgcgg tggccatcat cctgctcggc gcgggcgtcg gcgtgctcgg ctcgacggtg
    10021 aatgcctttg ccacggtcat tgcgtccgac gcggcgggga tacccttcac cacgggcatg
    10081 gtgctgcggc tcgtcctcct tggcctctgc tggctcgcct gcgtcatcta tgtgatgcgc
    10141 tatgccgaac gggtgcgcgc cgatccctcg cgctccatcg tctatgaccg gaaggaggaa
    10201 aacgagcggc atttcctctc ctccctcaag gcaaacgacc gtcccgagtt cacgacgctg
    10261 cacaaggtcg tgctcgccgt cttcgggctc accttcgtcg tcatgatctg gggcgtgctc
    10321 ttcggcggct ggtggatggg cgagatgacg gggctgttcc tggcttccgc catcctcatc
    10381 ggcctcatcg cccggcccgg cgagcaggaa ttcgtcgaag ctttcctgag cggcgtgcgc
    10441 gatctcgtcg gcgtggcgct catcatcggc cttgcccgcg gcatcgtcgt catcatggac
    10501 gccggccgga tcaccgacac tatccttcac tgggcggagg tttccgtcgc gggtctttcg
    10561 gaagttctct tcgtcaacgt catctactgg atcgaggtgc ttttgtcctt cttcgtgccc
    10621 tcctcttcgg gtctcgccgt cctaagcatg ccgatcatgg cgccggtggc cgattttgcc
    10681 ggtgtgggac gcgagctcgt ggtgaccgcc taccaatccg ccaacggcat tgtgaacctc
    10741 atcaatccca ccttcgcagt ggtgatggga gcgctcgcca tcggccgtgt gccctacgag
    10801 cgatggctcg ccttcatgtg gccgctgctt ctcatcctca ccgtcatcat catggcctgt
    10861 ctcagcgtgg ccgtcctcat ctgatggaga atgaccgtga cacagaccta tggtgtccat
    10921 tccgaagtgg gcaaactccg gcgcgtgctg gtccacaggc ccggcacagc cctcgcccgg
    10981 ctcaccccca ggaatcggga agagctgctc ttcgatgacg tgatctgggt caagcaggcg
    11041 cgggtcgagc acatgacgtt cgtcgatgcg atgcgctacc gcggagtcga ggtggtgttc
    11101 ctgcgcgaac tcctggccga gacgatgagg atagcggagg cgcggggctg gctgctcgac
    11161 cgaagggtga acgacaacac cgtaggcgtc gggcttgccg acgatctgca cgcctacctc
    11221 accgaaatcg acgccgacga actttccaac atcctgatgg gcggcatgag cagggccgaa
    11281 ctgccgatca aggcgaacag cgtcgttgca tcgatgctgg caccggagga gttcatcctg
    11341 ccgccgctac ccaaccatct gttcacgcgc gacaccacct gctggatcta tgagggcgtc
    11401 acgctcaacc cgatgcgctg gaacgcacgc cagctggaga cggtgaacat cgccgcgatc
    11461 tacaaattcc acccggattt tcgcgatgcc ggctttcccg tctggtgggg cgacccggac
    11521 cggaactacg gccgggccac ggcggaaggc ggggacgtga tgcccatcgg caaccgcacg
    11581 gtactgatcg gcatgggcga gcgcaccacg ccgcacgccg tgggacagat cgcccgggcg
    11641 ctcttcgcgc aaggcggggc ggaacgggtg atcgcctgcc ggctgcccgc cgaacgcgcc
    11701 agcatgcatc tcgatacggt cttcaccttc tgcgaccgcg acctcgtcac cgtcttcgca
    11761 ggcgtggtca aggacattca ggcgttctcc ctgcggccgg gcgacaagga atggcagatc
    11821 gacgtgcgcg cggaagagcg gccgctcacc gaggtcgtgg ccgaggcgct gggcctgaag
    11881 gagcttcgcg tcgtagagac cggaggagac gcctatgtgc gcgaacgcga gcaatgggac
    11941 gacggcaaca atgtcgtcgc gatcgagccc ggggtcgtca tcggatacga ccgcaacacc
    12001 tacacaaaca ccctcctgcg gaaggccggt gtcgaggtca tcaccgtgcc gggggcggag
    12061 ctcggccgcg gacgaggcgg gggccattgc atgacctgcc cgctgatccg cgaccccgca
    12121 tgaggagaga cagaaatgcc tgtgaacctc ctcggccaca atttcctgaa gctgctcgat
    12181 ttcacgccgg acgagatccg tttcctcctg cgcctcgccg cttcactgaa acaggcgaag
    12241 tacggcggct acgagcggca gacactgact aagaaaaaca ttgcgctgat cttcgagaag
    12301 gattccacgc gcacccgcac cggcttcgag gtcgcggcct atgaccaggg cgcgcatgtc
    12361 acctatctgg gaccgacggg cacgcagatc ggcaagaagg aatccatcaa ggacactgcc
    12421 cgcgtgctcg gccggattta tgacgccatc gaatatcgcg gcttcagcca ggagacggcg
    12481 gaggagcttg cgcgccacgc cggcgtgccc gtctacaacg gcctgaccga cgaattccac
    12541 cccacgcaga tcctcgccga cttcctgacg atgcgcgaat gcacgcgcaa gcatctctca
    12601 gaactcaagt tctgcttcct gggcgacgcc ggcaacaata tgggcaattc gctcctgagc
    12661 ggggccgcgc tgatcggcat ggacgtgcgg cttgccgcgc ccaaatcctg ctggccggac
    12721 gccgcgctcg tcgagcagtg ccggtcgatt gcagaaaaga ccggcgccag gatcacgctt
    12781 accgaggacc cgatggaggc ggcgcgcggc tgcgacttcg tctataccga tgtctgggtt
    12841 tcgatgggcg aaccggacga cgtctgggcc gaacggatca agctgcttac gccctatcgc
    12901 gtcacggccg aaatcatggc cgcaaccggg aacccgcacg ccaagttcat gcattgcctg
    12961 ccgtcctttc acaatcgcga gacggagatc ggcgaaagga ttttcaacca gtacggcatc
    13021 gactgcatgg aggtgacgga ggaggtcttc gaatcggaga catccgtggt cttcgaccag
    13081 gcggagaacc gcatgcacac catcaaggcg gtgctcgttg ccaccatagg aggctgaaat
    13141 gcgcgtcgtt gtggcgctcg gcggaaatgc gctgctcaag cgcggcgagc caatgacggc
    13201 cgaagtgcag cgcgccaacg tgcgcgtcgc agccgaggca cttgccgatc tcgcccgcga
    13261 tcacgagatc gtggttgcac acggcaacgg ccctcaagtg gggctgcttg cgctgcaggc
    13321 tgccgcctac aaaggcgtcg aaccctatcc gctcgatatc atcggcgctg agagcgtcgg
    13381 catgatcggc tatctggtcg agcaggaaat ggtcaacgcc atgcctcccg gcgctcgctg
    13441 cgccacgctt ttgacgcaga tcgaggtcga tcccgacgat cccgccttca aaaggccgcg
    13501 caagccgatc ggcccgcttt acgacaaagt ggaagcgcac cggctgcgct accagaagaa
    13561 gtggcccatg tgcgaggaga gcgagggcaa ctggcgccgc gtcgtcccct cgcccgagcc
    13621 gcgccgtatt gtcgagatca gtgtcatccg gcttctggtc gaagcgggcg ttacggtgat
    13681 ttgcgctggc ggcggaggca ttcccgtcat ccgcaatcgg aatggcgagc tggaaggtgt
    13741 ggaagccgtc atcgacaagg atcacgcagc agggctcctc gctcgcgaaa tcgatgccga
    13801 cgcgtttctt atgctgaccg acgtggaagc ggtttacgcc gattggcaaa cgccggcacc
    13861 acggccgatc cgagcgacca cgccggagga gctttcgggg atggcatttg ccgaaggctc
    13921 catgggcccc aaagtggagg cagcctgcgt cttcgctgca acacgcggaa ggatggccgg
    13981 gatcgggcgc ctgcaggacg cccgcgccat tctagaaggt cgtgccggta ctctggtgac
    14041 aaccgcaaac tgagcaccgg tcatcgtgcc atcagaacgg ccggtgcaaa cgcgcttgcg
    14101 tcatggagcg gaatagccgc catcgaccag gtggtaactc cccgtaatga aggatgcacg
    14161 gtctgacagc agaaaacagg tgagtgcggc cacttcctca gctctgccga gcctgccaat
    14221 cgggtgaaga ccgacaatct gcttcagctc atcgggtttg agattcttcg acaagagggg
    14281 cgtgtcgatg aagccgggcc cgacagcatt aatgcgaatg cctttggcgg catattcgat
    14341 tgccgccgtc ttggtgagac ccacaatgcc gtgtttcgcc gcgacatagg cgctcgaacg
    14401 cgcaaaaccg ttcgtgccga ggatcgacga catattgacg atggcgccgc cgcctgcctc
    14461 gagcatcgcg gggatttcat aacgcatgca gtagaacacg ctgctgagat ttatatcgat
    14521 gaccttgcgc cagccgtcgg gcgggtaatc accggtgagt tccgaagcgc cgctaatgcc
    14581 cgcgttgttg acggccaggt gtagctcgcc gaaggtcgac ttggcgaaga ccaccattgc
    14641 gtccacctcc tcggcaaccg ccacgtcgat acggaatgcg gcagccttgc cgccatccct
    14701 ttcgatttcc tcaacgattt tcttcgcgga cctctcatcg agatcggcga ccaccaccgc
    14761 cgctccggca gccgcgagct gccgcgcgca ttcagcgcct atccccgatg ccgcgcctgt
    14821 taccaaagcc accttgccct cgaactcatt cgccatgtgc agcaccgcct tctcgatcaa
    14881 caaaggaaat agcgtgcaaa gctgtcgcac caagtcatga cggacatttc agcataaacg
    14941 gcggcttgtg cgttgatgca gatcatcatt ttccgcctgg ccgcagcagg aggatgcccg
    15001 cctgcgctca ccggcgtccc gagtgagaag cctgcagtcg accctcgcga gaaagtgccc
    15061 ggcacggaag cggacagtgc cgccggaaca tcgtgcttct cggcgggacg gcgggagcca
    15121 ccgaccgata tccatctatc catcgtgcat ggaatagaag attgcactcc cgatccgtcg
    15181 gatcggtttc gtagcgcagg ttttctcggc tatattggcg gctagccggc gttcccggca
    15241 gaaggcaaga atggtcaatc cggcgggtag ggcactgcta taattctcct gcagcatcct
    15301 gtgtccgacg ctggggaatc gaggcgagcg catatggcgg cctgtgagga tgcgaaaccg
    15361 ggaacgcacc gattgctcaa aaatttcgcc cgcatcggcg cgactgaata cgaggacagc
    15421 caccaggccg tccgcaggat ggttctcgtc ggctcctcgg tgacctgtat tgcggccggc
    15481 ttgtgctgga gcgcgaccta tcttcttgcc ggcgctgtca tagcggccgc cgtgccggca
    15541 atctacgcag tgctcagcct ggcaagtact gccctgttcg cgttgacaag atcataccgt
    15601 atctatgtcg cgcttcagct caccctcatc ttcttccttc ctttgtgcat gtcggtcatt
    15661 ctcggcgacc aggctagtgc cgtggtcatc tggtcagcac ttgcgccatt cggcgcactg
    15721 ctgttcgcga gccgccgcac ggctctggta tggtttgcac tgctcctcct tgccctggcc
    15781 agtaccgtcg ttccgctgcc ggaaatcgaa cgtcctttgc cccacggttt cgtgacggca
    15841 atgtttgtgc tgaacatcgg cgcactgctt gccctgctgt ttgcgatgac ggcctatttc
    15901 atccggcaga tgcacctgct gcaggaccga tcggagatgc tgctgcacag catcctgccc
    15961 aagcacatcg ccgaacatct caaaggctct ccgagccgga ccgcaaagca gcatgacgag
    16021 gccagcatcc tgttcgccga cattgtcggc ttcactgcgc tttcatcaca attgagtccc
    16081 gacgagctgg tgaccatgct cgacgaggtg ttcgtcgaat tcgacctgct ggtggatcag
    16141 ctggggctcg agaagatcaa gaccatcggg gactgctaca tggtcgcttc cggcattcct
    16201 ctgccccgcg ccgaccatgc gcatgtgctg gcccagttgg cattggcaat gcagcgtacc
    16261 gtggcaaacc gcctgttctg cggccagcgc cttgcatttc ggatcggaat gaacagtggg
    16321 ccggtggtcg ccggcgtcat cggtcgcaag aagttcatct atgatctctg gggggaggcc
    16381 gtgaacatag ccagccgagt ggaaggcgga ggcacaggcg gtgcgataca gatatccgcc
    16441 gccacgcacg cgttgctcaa gggcgatttc gtttgcgtac ccgccggcaa ggtccgaatc
    16501 acgggcgagc atgaggtgga ggtctatcac ctgatcggcg aaaaaagatc gttgcctgat
    16561 ttcatcgggg agtcttccaa ggccggcaga cagcaattcg ccgatggcag actactcccg
    16621 tgagttcggt agccttcgta aaatcccttc tttccggtct aatcatcggc ctcacggcca
    16681 tcgtgttctc gatctccttc acagcgatca tctacactgg tgagcttgcg ccgtttctca
    16741 gccgcggtat cggattgacc cttgccggcg cggcagtcat ggccgtcgca ggcgccttta
    16801 cgctgtccta tcgcggcatc atcgtccagc ctcaggacgt gacggcgctg atcctttcgc
    16861 tcgctgccgc gtcaatcgcc ggcaactggc aaggtgctgc ttccgacgac cttttcgcga
    16921 cgatcgcggc gctggttgcg gtcgcgactg ccactaccgg cgtggctatg gttgtcttcg
    16981 ggcgtttgcg tctgggtcac atcgttcgct tcatccccta tcccgtgctc ggcggatttc
    17041 tcgcggcgac cgggtatctg cttgtgaccg gcgcgatcgg catgacgatc ggagacactg
    17101 tcagcgtccg gtccgccaac gttttgttcg acgccggcaa tccgcaaaaa tgggcgccct
    17161 ggatcgtggc agccgtcctg ttctacgtcg cggtgaagcg cgtgcggcat ggtctggtcc
    17221 tgccagcctg tttcgtcgcc gccgtcctgt gcttttacgg agctctcaaa cttttcggca
    17281 tcgaccaggc caccgcgcaa acgcggggcc tcctccttgg cccttttggg gaggcgaccc
    17341 tctatgcctc gctcgatccc ggccttcttg ttcgggcgcg gtgggatgtc gtcgcggcac
    17401 aactgccgtc gatcctcgcc gtcatcggca tggcgacgat cgggtcgctg ctcaatgtct
    17461 ccggacttga gttggccacg ggcgaggaca tcgatccgaa cagggagttg cacggcgttg
    17521 gagttgccaa ccttgcggcg tccgccgtcg gcgggatggt cggctatcac cttctcagcc
    17581 agacgctttt cgccaaggcg ctgggggtca ccggccgggc cgccggcatc tccgtggcct
    17641 tggtcacgct tgccgtgctg ttcctgggca cgacttatct gtccgttctg cccatcggcg
    17701 tgttcgcagc cgtgatcgcg tttctcggga tcgacctgct ggtcacctgg ctctgggtcg
    17761 agcggcgccg gctgcagctt cgcgattttt tcgtcatcgt cctgatcctc gccgtggcag
    17821 ccacaatcgg gttcctggaa gcgatcgcga ttggcattct cgcggcctcc accctcttca
    17881 tcgtcgccta ttcccgcgtg gacgtggtgc gcctgaagac gaccggcgcc agaaagcgct
    17941 ccggcgtcga acgtggtcag cgcgacctcg caatcctgtc ggatcgaggc gatcaggtgg
    18001 cgatctacga gctttccgga taccttttct tcggtacggc ccatcgtctg gtttccgaaa
    18061 tttcggaaga cacggtcgca caatcgaagg aattcgttct cgtggatttc aaacgcgtac
    18121 aaggcattga cgcttctgcg gcctttgcgc tgcgcagact cgtccagctc tgcgctgccg
    18181 gcagcgttac gctcattttc tgcggccttt ccgacaggct gtcacaaacg ctcgatcggg
    18241 ccggaatacc gtctgaacca tcgccgccgc ttctctttaa gcatctggac gacgccttgc
    18301 agatcgtcga ggaaaagctg cttctcgaca atgcaggacg tgagggcatc gagtcggaca
    18361 cgggcctgct ggacgagctt aggcggattg cccgaccgct cgatcctctc ggactgttcg
    18421 aggtttacga catcgggaaa ggcgaagttg tcatcgaaca gggcagcgag gccgacgaca
    18481 ttctcctgct cgtgagcgga gttctgcggg tcgaatatct gcgcgcggat ggcagcaaaa
    18541 ttcccgtcgc gaaaatcctt cccggagcgg tggtgggcga gatcggtttt tatgcgggag
    18601 agaggcgaac ggcccaagtg atcgccgagc aagactgccg gctcatgaaa atcagcaagc
    18661 ggacccttgc tgacctcgag gagtcacagc ccggtcttgt cagcaacgtt catcgcatcg
    18721 cggctaccaa tctggccttg cgcctcacgc gcgccatggc gctgttgcgc gacgcggacg
    18781 tctaaccaga tctcaagttt gcttgatcag catcgacaag gtcaatgcga ggcaaatctg
    18841 gtgcaagagc tgatcagcgc caacaacaat aaagaatgcg cgccactgtc cgctgcgcca
    18901 aaagcgggca gcgatcctcg aggtcgtgaa atcgacagcc atgtgaagca gtgcgttggc
    18961 gataacaaac gacaccagtg cgcccggtct cgctattcca aagatcacgc cggcgcccac
    19021 ggccaatgtg atgctgtagg tgatgacgtg agcaatcagc gcgtccaggc gatcgctctt
    19081 gttctccgcc atccacgacg actgcagaac gaagtcgccg atccagtgca cgaccagcag
    19141 acctgccatt tgccacaatg gtgcgagcgt ggatgacatg acgcatcctc atgctggtcg
    19201 gcaaagccgc gaaggcaact gccgttccgt ctcaatgtcg taccagctgt agccagaaca
    19261 gccacgcaag gccagtgcgc cgcctgccac gtcccacgct gcgccgccat aaaggccgat
    19321 atgccagctg tcggcagaac ccgaggaagc ccgcgcatct acatcgaaag aggaatggct
    19381 gtagcccgtc atgaagcgag gcggatattg tcggctaccg ccccgtcaag gccggcaagc
    19441 aagcccccgt ggaccaggag agcgaccggc attgccgtcg ccgtcaagct tgcgccaggc
    19501 gccgaaggcc ctgccccagg ccgccggcgc aagcgtgtcg gcagatgcca gagcccgccc
    19561 ggcaccctcc ggcccatagg ccagcaccgg cacggcgtc
//
LOCUS       DASVDU010000227        21412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_786994, whole genome shotgun sequence.
ACCESSION   DASVDU010000227 DASVDU010000000
VERSION     DASVDU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21412
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786994"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VF226_14435"
     CDS             complement(<1..1394)
                     /locus_tag="VF226_14435"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7075233.1"
                     /translation="MNVTNAPLLSLITFLPLVGVAFIAFLPRDAVQNARYIALWTTLV
                     TFAVSLLVWINFDTSTADFQFVEEHPWLGPIVYRMGVDGISMLFVILTTFLMPLCILA
                     SWESIQTRIKEYMIAFLVMETLMIGVFCALDLVLFYLFFEGSLIPMFLIIGVWGGPRR
                     VYASFKFFLYTLLGSLFMLLAMMAMYWHAGTTNIITLLHDVTFPGDMQWWLWLAFFAS
                     FAVKLPMWPVHTWLPDAHVEAPTAGSVILAAILLKMGGYGFLRFSVPMFPLASQELAP
                     LVFTLSVVAIIYTSLVALAQEDMKKLIAYSSVAHMGFVTMGIFTFTVQGLDGAIFQMI
                     SHGIVSAALFLCVGVIYDRMHTRDIDAYGGLVNRMPIYAVCFMVFTMANVGLPGTSGF
                     VGEFLVLLGAFGANTWVALFATTGIILGATYMLYLYRRIIFGPLVREELKRITDLSWR
                     EMAIMAPLVVLVLW"
     gene            complement(1391..3388)
                     /gene="nuoL"
                     /locus_tag="VF226_14440"
     CDS             complement(1391..3388)
                     /gene="nuoL"
                     /locus_tag="VF226_14440"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7075234.1"
                     /translation="MYQAIVFLPLIGAIVAGLFGRALGARPTEIFTTVLVGIAAVLSW
                     IAFIQVGFGGAVVKVPVARWITSGELDAAWSLRIDTLTAVMLVVVNTVSSLVHLYSIG
                     YMHEDESRERFFSFLSLFTFAMLMLVTADNLVQMFFGWEGVGLASYLLIGFWYTRPSA
                     NAAAIKAFVVNRVGDFGFALGIFGTFYVFQTVQLDAIFAAAPAFAGKTMNFLGYDVDI
                     PTVLCFLLFIGAMGKSAQFLLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPL
                     FEHTPTALQFVTLIGAVTAFFAATVGLVQNDIKRVIAYSTCSQLGYMFVACGVGAYSA
                     GIFHLFTHAFFKALLFLGAGSVIHAMHHEQDMRRMGGLADRIPITWAMMLIGTLALTG
                     VGIPGLIGFAGFHSKDAIIEAAWAANTPGNYAFWLLVIAALMTSFYSWRLFFLTFYGQ
                     PRWNVPGHSPGVIGGEPDLEHGHDHEPHESPPVMLVPLAVLALGSVVAGFAFSYYFIG
                     EGYSEFWGRALYTAPQSHILESMHHVPSVVAWSATIAMAIGFGVAWLYYMSATWLPAA
                     TARALKPAYDFLLNAWYFDALYDRLFVRPAFWIGRVLWKGGDGRVIDGIGPDGVAARV
                     LDATGRVVRLQTGYLYHYAFAMLIGIAIILTVFMFTGGQPL"
     gene            complement(3476..3793)
                     /gene="nuoK"
                     /locus_tag="VF226_14445"
     CDS             complement(3476..3793)
                     /gene="nuoK"
                     /locus_tag="VF226_14445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7075235.1"
                     /translation="MELSAVGLGHYLTVAALLFTLGIFGVFLNRKNVIIMLMSIELML
                     LAVNINLVAFSYYLGDLVGQVFALFVLTVAAAESAIGLAIVVVFFRNRGSIAVDDVSM
                     MKG"
     gene            complement(3830..4471)
                     /locus_tag="VF226_14450"
     CDS             complement(3830..4471)
                     /locus_tag="VF226_14450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7075236.1"
                     /translation="MGLAAFFFYLFAILSVLAGAMVIIAKNPVHAVLFLILAFFNAAG
                     LFVLLGAEFLAMVLVIVYVGAVAVLFLFVVMMLDVDFAALRAGFIRNAPLAALIGGIV
                     MVELVMVVGFAYIHPDVAGVAAAPLPTLGSGITNTEALGRLIYTRYVLLFQGAGLILL
                     VAMVGAIVLTLRHKPGVKRQSIAAQNARTPQTAIEVVKVEPGQAIVPSGETQG"
     gene            complement(4673..5164)
                     /gene="nuoI"
                     /locus_tag="VF226_14455"
     CDS             complement(4673..5164)
                     /gene="nuoI"
                     /locus_tag="VF226_14455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX7075237.1"
                     /translation="MASIAQAIRAGFLAEFVTAFILSMRYFFAPKATVNYPYEKGPVS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPAYAITIEAGPRRNDGTRRTTRYDIDMVK
                     CIYCGLCQEACPVDAIVEGPNFEFATETREELLYEKQRLLDNGDRWERAIAKNIALDA
                     KYR"
     gene            complement(5182..6246)
                     /gene="nuoH"
                     /locus_tag="VF226_14460"
     CDS             complement(5182..6246)
                     /gene="nuoH"
                     /locus_tag="VF226_14460"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7075238.1"
                     /translation="MTAYEIWWDYGWRLAIMLVQSLILLVGLLLVIAYLLLMDRKVWA
                     AVQMRRGPNVVGPFGLLQSFADLLKFVFKEPVIPAGAERAAFLLAPLATATFALAAWA
                     VIPLSEDGFGRWVISDLNVGILYILAISSLGVYGIIIGGWASNSKYPFMGSLRSAAQM
                     ISYEVSIGFVLITVLLVVGSLNLTDIVNSQRSGIGTAIGLPNTLIDWNWLVLLPMFVV
                     FFISALAETNRPPFDLPEAESELVAGYMVEYSSTPYLLFMLGEYVAITTMCALTTILF
                     LGGWLPPIDVAPFTWIPGIIWFLIKSVLVFFMFAMVKAIVPRYRYDQLMRLGWKVFLP
                     LSLAMVVVVAGVLHYGGLAP"
     gene            complement(6257..8356)
                     /gene="nuoG"
                     /locus_tag="VF226_14465"
     CDS             complement(6257..8356)
                     /gene="nuoG"
                     /locus_tag="VF226_14465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX7075239.1"
                     /translation="MPKQLIIDGQLIEVEDGLTLIQACEQAGVTIPRFCYHERLSIAG
                     NCRMCLVEVVGMPKPVASCAMGVSDLPPNKDGSPKVINTNTPMVKKAREGVLEFLLIN
                     HPLDCPICDQGGECDLQDQTMAFGLGGSRFQENKRAVEEKYISPLIRTFMTRCIHCTR
                     CVRFMTEVAGVEELGAIGRGEDMEITTYLEKGILSELSANVNDLCPVGALTHRPWSFL
                     ARPWELRHTESIDVTDALGSAIRVDSRGREVMRILPRNNDDVNEEWISDKTRHMADGL
                     RTQRLDQPYARKDGRLVPVAWDEALHIAADRLKAANPERIGAIAGDLAAAEEMFALKD
                     LMRRLGVASLDCRQYGEQLDPHLGRSSYLFNSTVVGIEDADALLIVGSNPRIECAVLN
                     ARILKRWRRGGMNVGLIGEAANLTYPYEHLGAGAQTLAELAAGRHSFMQVLADARRPM
                     VIVGSGAIARADGAAVLALAAQIALAAMQGKEPEWNGFNVLHAAAARVAGLDLGFVPG
                     EGGRDVLGMLKGDMELVYLLGADEIEMDGLGDAFVIYQGTHGDAAAHRADLIFPAAAY
                     TEKNGTYVNLEGRTQLTARAVMPPNEAKEDWTILRALSPLVGHTLPYDNLNALRTAMY
                     KEAPALARIGSVRPEGREGVEALAKRTGTTGAAPFKSIVKDFYLTNPIARASAVMAEL
                     SRLRALAASGRSAEAAE"
     gene            complement(8510..8698)
                     /locus_tag="VF226_14470"
     CDS             complement(8510..8698)
                     /locus_tag="VF226_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075240.1"
                     /translation="MTEPDNLVLRLLREIRERLDEQSDRFAAIDRRFYRLEKRFDETR
                     RYADRVLGIGRIQQGLPW"
     gene            complement(8704..9990)
                     /gene="nuoF"
                     /locus_tag="VF226_14475"
     CDS             complement(8704..9990)
                     /gene="nuoF"
                     /locus_tag="VF226_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7075241.1"
                     /translation="MLADKDRIFTNIYGFNDWRLAGARARGTWDNTKALLELGHEGII
                     DEVKKSGLRGRGGAGFPTGLKWSFMPKNDPRPSYLVVNADESEPGSCKDREILRHEPH
                     VLIEGCLVASFAMRAHTCFIYMRGEFIRERERLQAAVDEAYEARLIGKNNIHGWDLDI
                     IIHHGAGAYICGEETALLESLEGKKGLPRLKPPFPANVGLYGAPTTINNVESIAVVPT
                     ILRRGATWFAGLGKPNNTGTKLFQISGHVERPCVVEDEMGIPLRQLIEEHAGGVRGGW
                     NNLLAVIPGGSSMPLLPASMCDDLTMDFDSLQKLKSGLGTAAIIVMDKSTDIVKAITR
                     IAYFYKHESCGQCTPCREGTGWMWRVLERMSRGEAQKQEIDLLFDVSKQIEGHTICAF
                     GDAAAWPVQGLIRHFRHEIEARIDSYMVAGQRMAAE"
     gene            complement(10017..10631)
                     /gene="nuoE"
                     /locus_tag="VF226_14480"
     CDS             complement(10017..10631)
                     /gene="nuoE"
                     /locus_tag="VF226_14480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7075242.1"
                     /translation="MAVRRLDPNQPASFEFTPENLAWAKATIAKYPEGKQASAVIPLL
                     WRAQEQEGWLTEPAIRYVGDMLGMAYIRVYEVATFYTMFQLSPVGRKAHVQVCGTTPC
                     MLRGAEEIREVCRQRIHPEPHHLSEDGDFSWEEVECIACCANAPVVQIGKDTYEDLTP
                     ELFEKVLDGFARGEPPKPGSQIGRQASCPEGGPTTLKEFIKAND"
     gene            complement(10853..11257)
                     /locus_tag="VF226_14485"
     CDS             complement(10853..11257)
                     /locus_tag="VF226_14485"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEX7075243.1"
                     /translation="MAQRGEQRRKEVLLWLTIATGAVLSLIGARFLLQPEAAATFFGI
                     DRRNPGFAPHAAIALRDLWLGLLVIGFAVLRDWRAVALWLGLASLVCFGDAVIAATSS
                     GRWISVAFHSGSGLFCAGLAAFAWRQARPRAG"
     gene            complement(11413..12603)
                     /locus_tag="VF226_14490"
     CDS             complement(11413..12603)
                     /locus_tag="VF226_14490"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX7075244.1"
                     /translation="MPETDIRPFTINFGPQHPAAHGVLRLVLELDGEVVTRVDPHIGL
                     LHRGTEKLIESRTYLQNIGYFDRLDYVAPMNQEHAFCLAAEKLLGIKVPYRAQLIRVL
                     YCEIGRLLSHLLNVTTQAMDVGALTPPLWGFEEREKLMIFYERASGARMHAKYFRFGG
                     VHQDLPPELIEDIAKFCETHPKVLDDIHDLILENRIYKQRNVDIGVISLEDCMKWGFS
                     GVMLRGSGAAWDLRKAQPYECYAELDFDIPIGKNGDNYDRQLIRMEEMRQSVRIMKQC
                     CEKLLSAQGQGPHVADDRKVVPPKRARMKSRMEELIHHFKLYTEGFHVPEGEVYACVE
                     APKGEFGVYLVADGSNKPYRCKIRAPGFAHLQAMDFMNRGHMLADVSAILGSLDIVFG
                     EIDR"
     gene            complement(12675..13046)
                     /locus_tag="VF226_14495"
     CDS             complement(12675..13046)
                     /locus_tag="VF226_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX7075245.1"
                     /translation="MAGSIRSYKDLKVWQVAMSLAESCYRVTAELPRDEIYGMTTQIR
                     RSATAIPANIAEGYGRESTGAFIQFLRVAQGSLKELETHVMLAERVELISSEISVHLL
                     GECDRVSKMLRNLIRSLQERT"
     gene            complement(13086..13709)
                     /locus_tag="VF226_14500"
     CDS             complement(13086..13709)
                     /locus_tag="VF226_14500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7075246.1"
                     /translation="MAAEIETLRELGEYLQGKLGATLKSWHLRLGELTLNVDAAQIVP
                     VLKLLRDDGRCRFEILIDLCGVDWPGREKRFDVVYHLLSPRLNQRIRVKIETAEDDPV
                     ASAVEVFPAANWYEREAYDMYGILFAGHPDLRRILTDYGFQGYPLRKDFPLSGYVEVR
                     YDSEQKRVVYEPVRLTQQFRDFDFESPWEGTGRILPGDEKAESGMKS"
     gene            complement(13741..14310)
                     /locus_tag="VF226_14505"
     CDS             complement(13741..14310)
                     /locus_tag="VF226_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX7075247.1"
                     /translation="MGLILPPGPVFEKPREGGGTGLVTPTDPYFEQVSNELADKGFLV
                     TATDDLINWARTGSLMWMTFGLACCAVEMMQMSMPRWDAERFGFAPRASPRQSDVMIV
                     AGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYAVVRGCDRIIPVDVYV
                     PGCPPTAEALLYGVLLLQRKIRRTGTIER"
     gene            complement(14301..14666)
                     /locus_tag="VF226_14510"
     CDS             complement(14301..14666)
                     /locus_tag="VF226_14510"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX7075248.1"
                     /translation="MSPLLLDYLPLAVFIGVALFIGAALMVAPFLIAIRNPDPEKVSA
                     YECGFNAFDDARMKFDVRFYLVSILFIIFDIEVVFLFPWAVAFGDIGTFGFWSMIAFL
                     AVLTVGFIYEWKKGALEWD"
     gene            14997..15683
                     /locus_tag="VF226_14515"
     CDS             14997..15683
                     /locus_tag="VF226_14515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7075249.1"
                     /translation="MITGLLNTRSFRPASHRTVGSRYAPRRSEPLAATTIYFVRHAAH
                     TLLDKVLVGRKPGVPLSAEGLEQAHRLARQLAEKNISRVVSSPRERAVQTAAPIARAA
                     GVPMAISFSLDEIDLGAWTGLAFDELARDGNWNFWNMLRSIARPPEGESMSEVQERIV
                     RYLARLHVTHPGERVVLVSHAEVIRTAVLACQGRSLDSYADVQIPPAGVVELTIHDQG
                     AQLAAVGEAG"
     gene            15683..16846
                     /locus_tag="VF226_14520"
     CDS             15683..16846
                     /locus_tag="VF226_14520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075250.1"
                     /translation="MRLAIFGLTITSSWGNGHATLWRGLVRALARRGCRVTFFERDEP
                     YYSDNRDLSEIPGAEVVIYSHWDDVRAKAAAALTEADATIVTSYCHDGIAASELVHEK
                     ARGLSVFYDLDTPVTLARLDEGEPVDYIGPRGLRDFDLVLSYTGGRALQELQMRLGAR
                     RVVPLYGHVDPDVHQPVAPADRYRADLSYLGTYAADRQATLEALFVAPARKRPDQRFL
                     IGGAQYPADFPWSDNIYFVRHLPPSEHPAFFSSSRLTLNATRRAMADMGWCPSGRLFE
                     AAACGAAVISDTWAGLETFFTPGREILLAHDADDILSALVLTDAELARMAKAARERTL
                     DMHTSACRADELLEALSKAAHPEQPVRPAQELPLSIQGGIGDAPSHSDGRWQS"
     gene            16843..17658
                     /locus_tag="VF226_14525"
     CDS             16843..17658
                     /locus_tag="VF226_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7075251.1"
                     /translation="MTWGIVPAAGRGTRIQPLAFSKELLPVGSRLSGAGERPCAASEY
                     LLDRMLLAGANRICFVISPGKWDIMQYYGAEYGGAAIAYVMQPQPAGLCDAVFRAEPV
                     IRADEGVIIGLPDTIWFPETALAGLPEDQLAFLLFPVDRPEFFDAVILDGSGRVLEIQ
                     VKQANARSNWIWGAFRMPGHVFIELKRYWLARNRSDEYFGTLVNAWLAEGGTAVGIKA
                     GQAYVDVGTLNGYRAASALLTEQASSSAVADGEERLKRPTAPPSGGMLHEVSR"
     gene            17655..18428
                     /locus_tag="VF226_14530"
     CDS             17655..18428
                     /locus_tag="VF226_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HEX7075252.1"
                     /translation="MTTVETAPRSREEIARRIAELGPWFHNLELAGIKTAPDHFLGDY
                     PAIKARTFWDDVAARLPGRSVLDIGCNAGFYAIAAKRAGASRVVGIDADPDYLAQARF
                     AAEVCELDIDYQQLSVYDVGALGERFDVVLFLGVLYHLRHPLLALDLIHEHVAQDLLV
                     YQSMQRGSPHVAEVASDYDFWEAEHFEEPGYPKLHFIEHSYSGDPTNWWAPNRACSKA
                     MLRSAGFAILANPEEEVFICQRVAAPEGAGAVYPSRGHE"
     gene            18425..19351
                     /locus_tag="VF226_14535"
     CDS             18425..19351
                     /locus_tag="VF226_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEX7075253.1"
                     /translation="MIEAAMIWNEPNNKSHWDPELDPEWARFAEMANLAGKAIRRENP
                     NLLRVLGGISPIDPIFIRNMSQRGVLDNVDVVAVHGFPLDWNLWQIHEWPDKLEEIRA
                     ETELPVWVTEVGVSTFGAEEVQVWGLQRTAELLIGRTPRIHWYSLYDLPRTWEATTRH
                     KEAEGSSYYRHFHMGLLREDGTPKQALDKFARYTPDIGICQWFHFEDHRLDEAVAWMK
                     RLGVRYLRTGLSWADSFRPNALQWFDRQMEALADFDVTLTFCFTPEHRGIAPHHTSPP
                     LVAEEFAEFCASMVRRYCPGHRVPANQLMTGA"
     gene            complement(19335..20159)
                     /locus_tag="VF226_14540"
     CDS             complement(19335..20159)
                     /locus_tag="VF226_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075254.1"
                     /translation="MLIEKPAFAGQLREIVSRSHPKTLDRQIKRRQGYLLRPVCRHHL
                     SLQDLLIGRQVDYELLYVRCRIELLAIHSVSAASVIRHFDDSDGATSAFQQSRGSVYR
                     EGHRVMIRMSSFAGVGEKDVQPACDDDADQSVRQLDQPARGLLIRDPESDEGIAWHAC
                     GSQRIFQFLEPCFRVFGGGRKSCCASVVQVPGGTVRNQYETCARPARELGAAADRLVI
                     GMRENDTDVEGSDATSFLVEQKRNSLFRFLRWPAACFHRACCNSTEFVTQQITRQS"
     gene            20240..21310
                     /locus_tag="VF226_14545"
     CDS             20240..21310
                     /locus_tag="VF226_14545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075255.1"
                     /translation="MLRILQVAYSLAPVGPDAVGGSEQVLYHLERGLAARGFETLVIA
                     SEDSQVSGKLIPLPRASLPYDEPRIAAMRARQANAIRAALSRHRIDLVHMHGFDFDCY
                     LPPAGVPVLATLHCPPAWYSSNALYTSKPGVWLNAVSRYQFEDLSPDDQLLGFVENGV
                     PIEDLGNLHHARRRFALMLARISPEKGIREALEAARSADVPLLIAGSLYPYPDHQRYF
                     TEEIAPLLDRKRRYLGPVDFARKRHLLAAAQCVVIPSIEPETSSLVLREAFAAGTAVI
                     AFRQGALAEVIDHGVTGLLVDSVAEMAAALLTTSALDRDACRKIARERFSLERMIDGY
                     IQLYQEVLRRSASQIADFEIAR"
BASE COUNT         4114 a         6893 c         6703 g         3702 t
ORIGIN      
        1 atccacagca ccagaaccac gagaggcgcc atgatggcca tctcgcgcca gctgaggtcg
       61 gtgatccgct tcagctcctc gcggaccagc gggccgaaga tgatgcgccg gtagagatag
      121 agcatgtagg tggcgcccag gatgatacct gtcgtcgcga acagggcgac ccaggtgttc
      181 gcgccgaagg cgccgagcag gacgaggaac tcgccgacga agccgctggt gcccggcagg
      241 ccgacattgg ccatcgtgaa caccatgaag cagaccgcgt agatcggcat gcggttgacg
      301 agcccaccgt aggcgtcgat gtcgcgcgtg tgcatgcgat cgtaaatgac gccaacgcag
      361 aggaagagcg ccgccgacac gatgccgtgc gagatcatct ggaaaatggc accgtccagg
      421 ccctgaaccg tgaaggtgaa gatgcccatc gtcacgaagc ccatgtgggc cacggacgaa
      481 taggcgatca gcttcttcat gtcctcctgc gccagcgcca cgagagaggt gtagatgatc
      541 gccacaacgg agagcgtgaa caccagcggc gccagctcct ggctcgccaa tgggaacatc
      601 ggcacggaga agcgcaggaa gccgtagccg cccatcttca ggaggatcgc cgccaggatc
      661 accgagccgg ccgtcggcgc ctcgacgtgc gcgtcaggca gccacgtgtg gaccggccac
      721 atcggcagct tcactgcgaa ggaggcgaag aaggccagcc acagccacca ttgcatgtcg
      781 cccgggaagg tcacgtcatg caacaacgta atgatgttgg tcgtccccgc atgccagtac
      841 atggccatca tggccagcag catgaacagc gagccgagca gcgtgtagag gaagaacttg
      901 aagctggcat agacccgccg cggtccgccc cagacgccga tgatgaggaa catcgggatg
      961 aggcttccct cgaagaagag atagaagagc accaggtcga gcgcacagaa gacgccgatc
     1021 atcagcgtct ccatgacgag gaatgcgatc atgtattcct tgatgcgcgt ctggatgctt
     1081 tcccagctcg ccaggatgca cagaggcatc aggaacgtcg tcaggatgac gaacagcatg
     1141 gagatgccgt cgacccccat gcggtagacg atgggcccca gccaggggtg ctcctcgacg
     1201 aattggaagt ccgccgtcga cgtatcgaag ttgatccaaa cgagcagcga aacagcgaat
     1261 gtgacgagcg tggtccagag cgcgatatag cgcgcgttct ggactgcatc acgcggcagg
     1321 aaggcgatga acgcgacgcc gaccagcggc aggaacgtga tcagcgacag cagtggtgcg
     1381 ttggtgacgt tcatagcggc tgcccgcccg tgaacatgaa gacagtgagg atgatggcaa
     1441 ttccgatcag catcgcgaag gcataatggt agagataacc ggtctgcagc cgcacgaccc
     1501 ggccggtcgc atcgaggacc cgggcagcga cgccatccgg tccaatcccg tcgatcaccc
     1561 ggccatcgcc gcccttccag agcacgcgcc cgatccagaa cgcgggccgc acgaacagcc
     1621 ggtcatacag cgcgtcgaag taccaggcgt tgagcaggaa gtcgtaggcc ggcttgagcg
     1681 cccgtgccgt cgcggccggt agccacgtgg cggacatgta atagagccac gcgacgccaa
     1741 agccgatcgc catggcgatg gttgcggacc aagcgaccac ggacggcacg tggtgcatac
     1801 tctcgaggat atggctctga ggcgccgtgt agagcgcgcg gccccagaac tcagagtacc
     1861 cctcgccgat gaagtaatag ctgaaggcga acccagcgac gactgagccg agcgccaaca
     1921 ccgcgagggg caccagcatc accggcggac tctcgtgggg ctcgtgatca tgcccgtgct
     1981 ccagatccgg ctcgccgccg atcacgcccg ggctgtggcc tggcacattc cagcgtggct
     2041 ggccgtagaa ggtcaggaag aacagacgcc aggaatagaa cgaggtcatg agagcggcga
     2101 tgaccagcag ccagaaggcg tagttgccgg gcgtgttcgc ggcccaagcc gcttcgatga
     2161 tggcgtcctt cgaatggaag ccggcaaagc cgatgaggcc ggggatgccg acgcccgtaa
     2221 gagccagtgt accaatcagc atcatcgccc aggtgatggg gatgcggtcc gccagaccac
     2281 ccatgcggcg catatcctgc tcgtggtgca tggcatggat caccgagccg gcgccaagga
     2341 acagcagggc cttgaagaag gcgtgcgtga acaggtggaa gatgcctgcc gagtaggcgc
     2401 cgaccccgca ggccacgaac atgtagccga gctgagagca ggtcgaatag gcgatgacgc
     2461 gcttgatgtc gttctgcacg aggccgacgg tcgctgcgaa gaaagccgtc acggcgccga
     2521 tcaacgtcac gaactgaagc gcggtcggcg tgtgctcgaa aagcggtgag aggcgcgcca
     2581 ccatgaacac gccggccgtc accatcgtcg cggcatggat gagggccgac acgggggtcg
     2641 gaccctccat ggcgtccggc aaccacgtgt gcagcaggaa ttgcgccgac ttgcccatgg
     2701 cgccgatgaa cagcaggaag cagagcactg tcgggatatc gacgtcgtag cccaggaagt
     2761 tcatcgtctt cccggcgaac gccggcgcgg cagcgaagat ggcatcgagc tgaaccgtct
     2821 ggaagacgta gaacgtaccg aagatgccga gcgcgaagcc gaagtcacca acgcggttga
     2881 cgacgaaggc cttgatggca gccgcgttgg cgctggggcg cgtgtaccag aagccgatga
     2941 gcaggtagct cgccagaccg acaccttccc acccgaagaa catctgcacg aggttgtcgg
     3001 ccgtcaccag catcagcatc gcgaaggtga acagcgacag aaaggagaag aaccgctcgc
     3061 ggctctcgtc ctcgtgcata tagccgatgg aatagaggtg gacgagcgag gagaccgtgt
     3121 tgacgaccac cagcatcacg gcggtcagcg tgtcgatgcg cagagaccac gcggcatcga
     3181 gctcgcccga ggtaatccag cgcgcgaccg gcaccttcac gactgcgccg ccgaagccga
     3241 cctgaatgaa ggcgatccac gacagcaccg ccgcaatgcc gaccagaacc gtcgtgaaaa
     3301 tctccgtcgg acgggccccc agcgcgcgcc cgaaaagacc cgcgacgatc gcgccgatca
     3361 ggggcaggaa gacgatggcc tgatagatca tcgcgccacc ttgccgatca cgtgacagga
     3421 cagactgaag ccagcacccg ccgcttcctg ccgattgcaa accatctaag ccccctcagc
     3481 ccttcatcat gctgacgtcg tcgacggcga tcgagccgcg gttacggaag aagacgacca
     3541 cgatcgcaag gccgatcgcc gactccgcgg ccgctactgt cagcacgaac agagcgaaca
     3601 cctgccccac gaggtcgccg agatagtacg agaacgccac gagattgatg ttgaccgcga
     3661 gcagcatcag ctcgatcgac atcagcatga tgatgacgtt cttgcggttg aggaacacgc
     3721 cgaaaatacc gagcgtgaag agcagcgccg cgactgtgag ataatggccg agcccgaccg
     3781 ccgagagttc catgttggtg tcccgatccg atcccttgag tatgcagcct cagccctgcg
     3841 tctcgcccga cggcacgatc gcctgaccag gctcgacctt caccacctcg atcgcagttt
     3901 gcggcgtgcg cgcgttctgc gccgcgatcg actgccgctt cacgcccggc ttgtgccgca
     3961 aggtcaagac gatggccccg accatggcca cgagcagtat gaggccggcc ccctggaaca
     4021 ggaggacata gcgcgtgtag atgaggcggc cgagcgcctc ggtattggtg atgcctgagc
     4081 cgagcgtcgg cagcggcgcc gccgcgaccc ctgcgacgtc cgggtggatg taggcgaagc
     4141 ccaccaccat gacgagctcg accatgacga tcccgccgat gagcgcggcc agaggagcgt
     4201 tgcgaatgaa cccagccctc agcgcggcga aatcgacgtc gagcatcatg accacgaaca
     4261 ggaacagcac cgcgaccgcg ccgacgtaga cgatcaccag caccatcgcc aggaactcgg
     4321 cgccgaggag gacgaaaagt cccgccgcgt tgaagaacgc caggatcagg aagagaacag
     4381 cgtgcacggg gttcttcgcg atgatcacca tggcgcccgc gagcacgctc aatatcgcga
     4441 agagatagaa gaagaatgcc gccaggccca tttttgcgct ttccgtccgg ttccccttca
     4501 gccgccccgg cccgagctgc tgtcacccat gaattttccg acgccccgaa gcagccggtc
     4561 cggctccgca cgggcactta gcccaaccgg cgccataaac caagaccgaa aaggcccatc
     4621 tgcgggaccc ggatgcatgg cctctgcctc cgaaccagcc ctgttctctc ggtcagcggt
     4681 acttggcgtc gagcgcgatg ttcttggcga tcgcccgctc ccagcgatcg ccgttgtcga
     4741 gcaggcgctg cttctcgtac agcagctcct cgcgggtttc cgtcgcgaac tcgaagttcg
     4801 ggccctcgac gatcgcatcg accgggcagg cctcctggca caacccgcag tagatgcatt
     4861 taaccatatc gatgtcatag cgcgtggtgc gccgcgtgcc gtcgttacgg cgcggacctg
     4921 cttcgatggt gatggcgtat gcgggacaga tcgcctcgca cagcttgcag gcgatgcagc
     4981 gctcttcgcc attggggtag cgccgcaagg catgctcgcc acggaaccgc ggactcaccg
     5041 gtcccttctc atacgggtag ttcaccgtcg ccttgggcgc aaagaagtag cgcatgctca
     5101 ggatgaaggc agtaacgaac tctgcgagga agcctgcgcg aattgcttgt gcgattgatg
     5161 ccataggaat tcactccgcc cttatggcgc gagtccgccg tagtgaagga cgccggcgac
     5221 caccacgacc atcgcgagcg acagcggcag gaacaccttc cagccgagcc gcatgagctg
     5281 gtcatagcgg taacgcggga cgatcgcctt caccattgcg aacatgaaga aaacgagcac
     5341 ggacttgatg aggaaccaga tgattccggg gatccaggtg aacggcgcca catcgatcgg
     5401 cggcagccag ccccccagaa acaggatcgt cgtcagcgcg cacatggtcg tgatcgccac
     5461 gtactcgccg agcatgaaca gcaggtacgg cgtggaggag tactccacca tgtagccggc
     5521 cacgagctcc gattcggctt ccggcaggtc gaacggcgga cggttcgtct cggcgagcgc
     5581 cgagatgaag aacacaacga acatcggcag cagcacgagc cagttccagt cgatcagggt
     5641 attcggcagg ccgatcgccg taccgatgcc gctgcgctgc gaattgacga tgtcggtcag
     5701 attgagcgag ccgacgacga gcagaacggt gatgagcacg aagccgatgg agacctcgta
     5761 ggagatcatc tgcgccgcgg agcgcagcga gcccatgaac gggtacttcg agttcgatgc
     5821 ccagccaccg atgatgatgc cgtagacgcc gagcgatgag atcgcgagaa tgtagaggat
     5881 gccgacgttg aggtcggaga tgacccagcg gccgaacccg tcctcgctca acgggatcac
     5941 ggcccaggcg gcgagcgcaa aggttgcggt cgccagcggc gccagaagaa aagcggcgcg
     6001 ctccgcgccc gccggaatga ccggctcctt gaagacgaac ttcagcaggt cggcgaacga
     6061 ttgaaggagg ccgaagggcc ccaccacgtt cgggccgcgc cgcatctgaa cggccgccca
     6121 aaccttccgg tccatgagca gcagataggc gatgaccagc aacaaaccga ccagcagaat
     6181 caggctctgc acaagcatga tcgccagccg ccaaccgtag tcccaccaga tttcgtaagc
     6241 ggtcattgaa gccgtcctat tccgccgcct cggcgctgcg gcccgatgca gcgagcgcgc
     6301 ggaggcgact gagctcggcc atcaccgcgg acgcacgggc gatcgggttg gtcagataga
     6361 aatctttcac gatggacttg aagggcgcag cgccggtggt gccggtccgc ttcgcgagcg
     6421 cttcgacccc ctctcggccc tctggcctca cgctgccgat acgggcaagc gccggcgcct
     6481 ccttgtacat ggcggtgcgc agcgcattca gattgtcgta gggcagcgtg tggccgacca
     6541 gcggcgaaag cgcccgcaga atggtccagt cctctttggc ctcgttcggc ggcatgaccg
     6601 cgcgggcggt caactgggtg cggccttcga gattgacgta agttccattc ttttcggtgt
     6661 aggccgccgc agggaagatc aggtcggccc gatgcgcggc cgcgtcgccg tgtgttccct
     6721 gataaatgac gaaggcatcg ccaaggccat ccatctcgat ctcgtcggcg ccgagcaggt
     6781 agacgagctc catgtcgccc ttcaacatgc cgaggacgtc gcgcccgccc tcgcccggca
     6841 cgaaaccgag gtcgaggccc gcgacgcggg ctgccgcagc atggagcacg ttgaagccgt
     6901 tccattccgg ctccttgccc tgcatggcag cgagcgcaat ctgcgcagcg agcgcgagta
     6961 ccgcggcacc gtcggcgcgt gcgatggcac cgctccccac gatgaccatg ggccggcggg
     7021 catcggcaag cacctgcatg aacgagtggc ggcccgccgc cagctccgcc aaggtctgcg
     7081 cgcccgcgcc gaggtgctcg tagggatagg tcaggttcgc ggcctcaccg atgagcccga
     7141 cattcatgcc gccgcggcgc cagcgtttca gaatgcgcgc gttgagaacg gcacactcga
     7201 tgcgcggatt ggatccgacg atcagaaggg catcggcatc ctcgatcccg acgacggtcg
     7261 aattgaaaag atagctgctg cggccgagat gcggatcgag ttgctcgccg tactgccggc
     7321 agtcgagact ggcaacgccg agccgccgca tcagatcctt gagcgcgaac atctcttccg
     7381 ccgcggcgag gtcgccggcg atggcgccga tgcgctccgg attcgccgct ttcagcctgt
     7441 cggccgcaat gtgcaaggct tcgtcccagg ccaccggcac gagccggccg tccttgcgcg
     7501 catagggctg atcgagccgc tgcgtgcgca ggccgtcggc catatggcgg gtcttatccg
     7561 aaatccactc ctcattcacg tcgtcgttgt tgcgcggcag gatgcgcatc acctcccggc
     7621 cgcgcgagtc gacgcgaatg gcggagccca gcgcatccgt cacgtcaatc gactcggtat
     7681 gcctgagctc ccagggccgt gccaggaacg accacgggcg gtgcgtcagg gcgccaacgg
     7741 ggcaaaggtc gttcacgttg gccgaaagct cggagaggat gcccttttcg aggtacgtcg
     7801 tgatttccat gtcctcgccg cgccctatcg cgccgagctc ctcgacgccc gcgacttccg
     7861 tcatgaagcg gacgcagcgc gtgcagtgga tgcagcgggt catgaatgtc cggatcagcg
     7921 gactgatata cttctcctcg accgcgcgct tgttctcctg aaagcgagag ccgccgaggc
     7981 cgaaggccat cgtctggtcc tgcaggtcgc attcgccacc ctgatcgcag atcggacaat
     8041 cgagcgggtg gttgatgagc agaaattcca gaacaccttc gcgcgccttc ttcaccatcg
     8101 gcgtgttggt gttgatcacc ttggggctgc cgtccttgtt cggcggcaga tcgctgacgc
     8161 ccatggcgca ggaggcaacc ggcttcggca tgccgaccac ctcgacgagg cacatacggc
     8221 agttgccggc gatcgacaga cgctcgtggt agcagaagcg ggggatggtc acgcccgcct
     8281 gctcgcaagc ctggatcagc gtgaggccat cctcgacctc gatgagctgc ccgtcgatga
     8341 tgagttgttt cggcattcta atctctcaaa ctcgacccag cttccggagg cagaatactt
     8401 tggtcatccc ggcgcttgtc gccgggatcc accacgccgc ttgctccggt gcccgtggtt
     8461 ggctggatcc cgggcacaag gcccgggatg acgaatcctg gggaacctat caccacggca
     8521 acccctgctg aattcgtcca attccaagca cgcggtcggc gtatctgcgc gtctcgtcaa
     8581 accgcttctc cagccgatag aaacggcgat cgatcgcagc aaaccgatcg gactgctcat
     8641 caagcctttc gcggatttcg cgaagcagcc gcaggacgag gttgtctggc tcggtcatgg
     8701 ttcctactcc gccgccatcc gctggccggc caccatatag ctgtctatac gagcctcgat
     8761 ctcatggcgg aaatggcgga tgagaccctg caccggccac gcagccgcat cgccgaacgc
     8821 gcagatcgta tggccctcga tctgctttga gacatcgaac agcaggtcga tttcctgctt
     8881 ctgcgcctca ccgcggctca tgcgttccag cacccgccac atccagccgg tgccctcgcg
     8941 acacggcgtg cactggccgc agctttcgtg tttatagaaa taggcgatgc gcgtgatcgc
     9001 cttcacgatg tcggtagact tgtccatgac gatgatcgcc gccgtgccga ggccggactt
     9061 cagcttctgc agcgaatcga aatccatggt caggtcgtcg cacatcgagg cgggcagcag
     9121 cggcatcgac gagccgccag gaatgaccgc gagcaggttg ttccagccgc cgcggacgcc
     9181 gccggcgtgc tcctcgatga gctggcgcag cggaattccc atctcgtcct cgaccacgca
     9241 cggccgctcg acgtggcccg agatctggaa cagcttcgtg cctgtgttat tcggcttgcc
     9301 gagccctgcg aaccaggtcg cgccgcggcg cagaatggtc ggcacgaccg cgatcgactc
     9361 gacgttgttg atcgtcgtcg gcgcgccgta gaggccgacg ttggccggaa acggcggctt
     9421 cagccgcggc aagcccttct tgccctcgag gctttcgagc aaggccgtct cctcgccgca
     9481 gatgtaggcg ccggcgccgt ggtggataat gatgtcgagg tcccagccgt ggatgttgtt
     9541 cttgccgatc agccgcgcct cgtaggcctc gtcgaccgcc gcctgcaggc gctcgcgctc
     9601 gcggatgaac tcgccgcgca tgtagatgaa gcaggtgtgg gcacgcatgg cgaagctggc
     9661 gacgaggcag ccttcgatca gcacgtgcgg ctcgtggcgc aggatctcgc gatccttgca
     9721 ggatccgggc tccgattcgt cagcgttgac caccaggtag ctggggcgcg ggtcgttctt
     9781 gggcatgaac gaccacttga gcccggtcgg aaagccggcg ccgccgcgtc ccctcaggcc
     9841 agacttcttc acttcgtcga tgatgccctc gtggcccagc tcgagcagag ccttggtatt
     9901 gtcccaggtg ccgcgcgccc gcgcaccggc aagtcgccag tcgttgaaac cgtagatgtt
     9961 ggtgaagatg cgatccttgt ccgcgagcat cgcgtgcctt ccggtcttac gtggcgtcag
    10021 tcgttcgcct tgatgaattc cttcagcgtc gtcggcccgc cctcgggaca cgatgcctgc
    10081 cggccaatct gcgagcccgg cttcggcggc tcacctctgg cgaacccgtc gagcaccttc
    10141 tcgaacagct ccggcgtcag atcctcgtag gtgtccttgc cgatctgcac caccggcgca
    10201 ttggcgcaac aggcgatgca ctcgacctct tcccaggaga aatccccgtc ctcggacagg
    10261 tgatggggct ccggatgaat gcgctgccgg cacacctccc ggatttcctc ggccccgcgc
    10321 agcatgcagg gagtcgtgcc gcagacctgg acgtgcgcct tgcggcccac cggcgacaac
    10381 tggaacatcg tgtagaacgt ggcgacctcg tagacccgga tataggccat gccgagcatg
    10441 tcgcccacat aacggatcgc cggctcggtc agccagccct cctgctcctg agcacgccac
    10501 agcaacggga tcaccgccga ggcctgctta ccttccggat acttggcgat cgtcgccttt
    10561 gcccaggcca aattctcggg cgtgaattcg aaactggcag gctggttcgg gtcgagacgt
    10621 cggacggcca tggctgctcg gttccaatgg gatcggcaat agtcgctcgc gatcgcagaa
    10681 ttcgcgcccg ggggaaggtg ccccgccaaa ccggcgcttc cagtgacgcg tcgtcccacc
    10741 ccgcaagcgg tgctcttatg gcccagggat cgctgcgtcg caagataact cgtcggccta
    10801 tgaccatttg cagcaacgga ttcgttgcgg ccttgcccat ggtcaagccg ggtcagccgg
    10861 cacgcggacg cgcttgacgc cacgcgaacg ccgcgagacc ggcgcagaaa agccctgatc
    10921 cgctatgaaa ggcgacggat atccaacggc cgctcgacgt ggcggcgatg accgcatccc
    10981 cgaaacacac caatgacgcc agacccagcc acaacgcgac ggcacgccag tccctgagca
    11041 ccgcgaatcc gatcaccagc agaccgagcc ataggtcgcg cagtgcgatc gccgcgtgcg
    11101 gggcaaaacc ggggttgcgg cgatcgatgc cgaagaaggt ggcggccgct tcgggttgca
    11161 gcagaaagcg cgcgccgatg aggctgagca ccgcgccggt ggcgatggtg agccagagga
    11221 gaacttcctt gcgccgctgt tctcctcgct gtgccacctc gccccctgcg cagcgaagat
    11281 actacttcaa tgtcatccca gcgcttgtcg ccgggatcca ccttgccgct cgctttggag
    11341 cctgcggata gatggatccc gggcacaagg cccgggatga cttggtgagc agaccggcct
    11401 tcggcatcac catcaccggt cgatttcacc gaacacgatg tcgagcgagc cgaggatggc
    11461 cgagacgtca gccagcatgt ggccgcgatt catgaagtcc atcgcttgca ggtgagcgaa
    11521 gccaggagcg cgaatcttgc accgatacgg cttgttgctg ccatccgcca ccagatagac
    11581 accgaactct cccttcggcg cctcgacgca agcgtaaacc tcaccctccg gcacgtggaa
    11641 gccctcggtg tagagcttga agtggtggat gagctcctcc atcctgctct tcatccgcgc
    11701 gcgcttggga ggcaccacct tgcgatcgtc cgcgacgtgc ggcccctggc cctgcgccga
    11761 cagcagcttc tcgcagcact gcttcatgat gcgtaccgac tggcgcatct cctccatgcg
    11821 gatgagctgc cggtcgtagt tgtcgccgtt cttgccgatg ggtatatcga aatccagctc
    11881 ggcgtagcat tcgtaaggct gagccttgcg caggtcccag gccgctccgg agccgcgcag
    11941 catgaccccc gagaagcccc acttcatgca gtcctcgagc gagatgacgc cgatatcgac
    12001 gttccgctgc ttgtaaatgc ggttctcgag aataaggtcg tggatatcgt cgagaacctt
    12061 cgggtgagtc tcgcagaact tggcgatgtc ctcgatcagc tccggcggca ggtcctggtg
    12121 cacgccgcca aagcggaaat acttggcgtg catgcgggcg cccgaggcgc gctcgtaaaa
    12181 gatcatcagc ttctcgcgct cctcgaagcc ccacagcggt ggggtcaacg cgcccacgtc
    12241 catcgcctgt gtggtgacgt tgaggagatg cgacagaagc cgtccgattt cgcagtagag
    12301 cacccggatg agctgcgccc gatagggtac ctttattccg agcagcttct cggccgccaa
    12361 acagaaggca tgctcctggt tcatcggcgc gacgtagtcg agccggtcga agtagccgat
    12421 gttctgcaga taggtgcgcg actcgatcag tttctcggtg ccgcgatgga gcaacccgat
    12481 gtgcgggtcg acccgcgtga cgacctcacc atccagctcc agcacaaggc gcaacacccc
    12541 gtgtgccgcc ggatgctggg gcccgaagtt gatggtgaat gggcgaatgt cggtttctgg
    12601 cataagcggt ccgtgaagtc gtgaaatcgt gaagtcgcca aatcgtggtc gtgcagaagg
    12661 ctgagtgatg tgcttcaggt cctttcctga agcgaccgga tcagattgcg aagcatcttg
    12721 ctgacacgat cgcattcgcc gagcagatga accgaaattt cgctgctgat cagctctact
    12781 ctttctgcga gcatgacgtg cgtctcaagt tctttcaacg agccctgagc cactctcagg
    12841 aactgaatga acgcccctgt actttctcgt ccatatcctt cggctatgtt tgccggaatc
    12901 gcagtcgccg agcgccggat ctgcgtggtc atcccataga tttcatcgcg cgggagttcc
    12961 gccgtgacgc gatagcagct ctcggccaga gacatcgcca cttgccagac cttcagatct
    13021 ttgtaggagc gaatgctccc agccaccgac tccccccaac gtcacgactt cacgagttgg
    13081 cgatttcacg atttcatgcc tgactcagcc ttctcatccc cgggcaaaat ccgccccgtt
    13141 ccttcccatg ggctctcgaa atcaaagtcg cggaactgct gggtcagccg caccggctcg
    13201 taaacgaccc tcttctgctc gctgtcataa cggacctcga catagccgct gagcgggaag
    13261 tccttgcgta gggggtaacc ctggaaaccg tagtcggtga gaatacggcg cagatccggg
    13321 tggccggcga acaggatgcc gtacatgtcg taggcttccc gctcgtacca gttcgccgct
    13381 ggaaagactt cgaccgcgct cgcgaccgga tcatcttcgg cagtctcgat cttcacccgg
    13441 atgcgctggt tgaggcgcgg cgacagcagg tgatagacga cgtcgaagcg cttctcacgg
    13501 ccgggccaat cgactccaca gaggtcgatc aggatttcga agcggcagcg gccgtcatca
    13561 cggagcagct tcagcaccgg cacgatctgc gccgcgtcca cattcagcgt cagctcgccg
    13621 aggcgcagat gccagctctt gagggtcgcg ccgagcttgc cctgaaggta ttcgcccagc
    13681 tcgcgcaggg tttctatctc agccgccatt ttgacttctt ctcccctcgc gccgcctgca
    13741 tcagcgctcg atggtgccgg tgcggcgaat tttcctctgc agcaggagga caccgtagag
    13801 cagagcctcc gccgtgggcg ggcagccggg cacgtagacg tcgaccggga tgatgcggtc
    13861 gcatccgcgc acgaccgcat aggaataatg gtagtatccg ccgccgttgg cgcagcttcc
    13921 catcgagatc acgtagcgcg gctctggcat ctggtcatag accttgcgga gcgcgggcgc
    13981 catcttgttg gtgagcgtgc cggcgacgat catgacgtcc gactgacggg gcgaggcgcg
    14041 cggcgcgaag ccgaaccgct cagcatccca gcgcggcatc gacatctgca tcatctcgac
    14101 ggcgcagcag gccagcccga acgtcatcca catcagcgag ccggtacgcg cccagttgat
    14161 gagatcgtcg gtggcggtga cgagaaagcc cttgtcggcc agttcgttcg agacctgctc
    14221 gaaataggga tcggtcggtg tgacgagtcc cgtcccaccg ccttcgcgtg gcttctcgaa
    14281 cacaggaccg ggaggcagga tcaatcccat tcgagggctc ccttcttcca ctcgtagatg
    14341 aagccgaccg tcaacacggc caggaaggcg atcatcgacc agaacccgaa cgtgccgatg
    14401 tccccgaacg ccaccgccca ggggaacagg aacacgacct cgatgtcgaa gatgatgaag
    14461 aggatcgaaa caaggtagaa gcgcacatcg aacttcatgc gcgcgtcgtc gaaggcgttg
    14521 aagccgcact cgtaggccga aaccttctcc ggatcagggt tgcggatggc gatcagaaac
    14581 ggagcgacca tcagcgcggc gccaatgaac agcgcgacac cgatgaacac cgcaagcggc
    14641 agatagtcca agagaagcgg gctcatggtc gaatcttcct gcgacggtgc cggtcaagtg
    14701 aggcaccggt gcacgctcgt aacctacgag ccgcgggcgt gcaacagtca cgcacggcat
    14761 ctggtgccaa aagaggcctc ttccgagctg gccgaaggtc gcagccgggg gcttgagtgg
    14821 catggatgca tttcggggca tcaggttgaa acccgttacc caagcctgca accaggccgg
    14881 cccacgccgg aaccaatcca gcattcctcg cgttcgctag gctgatcacg gcctattcgg
    14941 ctcgtgctcc aacgagaaag aatgcgtccg aggtggggcg gggatcggag cacggcatga
    15001 tcactggcct cctgaacacg cggtcctttc gacccgcctc ccatcgaacc gtggggagcc
    15061 gctacgcgcc ccgccgctcc gagccgctcg ccgcgacgac gatctacttc gtgcggcatg
    15121 ccgcccacac tctgctcgac aaggtcctcg tcggccgcaa gcccggcgtg ccgctcagtg
    15181 ccgagggcct ggagcaggcg catcgcctcg cgcggcaact ggccgagaag aacatctcgc
    15241 gtgtggtctc aagcccccgg gagcgggccg ttcagacggc cgcgccgatt gcacgcgccg
    15301 ccggggtgcc catggcgatc tccttctccc tcgacgagat cgacctcggg gcttggacgg
    15361 gtcttgcttt cgatgagttg gcaagagacg gaaactggaa tttctggaac atgctgcgca
    15421 gcatcgcccg cccgccggaa ggtgagagca tgagcgaggt gcaggagcgc atcgtgcgct
    15481 atctggctcg cctgcatgtc acgcatccgg gcgagcgcgt cgtgctcgtc agccatgccg
    15541 aggtcattcg cacggccgtg ctcgcctgcc aggggcgctc ccttgactcc tatgccgacg
    15601 tgcagatccc gcccgccggc gtcgtcgaac tcacgatcca cgaccagggc gcacagcttg
    15661 cggccgttgg agaggcgggc tgatgcggct cgccatcttc ggcctgacca tcacctcgtc
    15721 ctgggggaac ggtcatgcca cgctctggcg cgggctcgtt cgggcgctgg ctcggcgcgg
    15781 gtgccgcgtg accttcttcg aacgcgatga gccctactat tccgacaacc gcgacctttc
    15841 cgagatcccc ggtgccgagg ttgtcatcta ctcccactgg gatgacgttc gcgccaaggc
    15901 cgcagccgct ctgaccgagg ccgacgcgac catcgtcacc tcctattgcc atgacggcat
    15961 cgccgcgtcg gagctggtgc acgagaaggc ccgaggcctt tccgtgttct atgatctcga
    16021 cacgccagtg acgttggccc ggctcgacga aggcgagccg gtggactaca tcgggccgcg
    16081 cgggctgcgc gatttcgatc tcgtcttgag ctacacgggc ggccgggcgc tccaagagtt
    16141 gcaaatgcgc cttggcgcgc ggcgggtcgt gccgctctac gggcacgtcg accccgatgt
    16201 gcatcagccc gtggcgccgg ccgatcgcta ccgtgccgac ctgtcatatc tcggcaccta
    16261 tgctgccgac cggcaagcca ccctggaggc gctgtttgtg gcgccggccc gcaaacggcc
    16321 ggaccagaga ttcctgatcg gcggcgccca gtatccggcc gacttcccat ggtcggacaa
    16381 catctacttc gtccggcacc tgcccccgtc ggagcacccg gccttcttct cctcctcgcg
    16441 cctgacgctc aatgctaccc gccgcgccat ggcggacatg ggctggtgcc cctcgggccg
    16501 cctcttcgaa gctgccgcct gcggtgcggc ggtcatctcc gatacctggg cgggtctcga
    16561 gacattcttc acgcccggcc gcgagatcct gctggcgcac gatgcagacg atatcctgag
    16621 tgcattagtg ctaacggacg ccgagctcgc ccgcatggcg aaagcggccc gcgagcggac
    16681 actcgacatg cacacgtcgg cctgccgggc ggatgagctg ctcgaagcgc tgtcgaaggc
    16741 tgcccatccg gagcagccgg tccggcctgc gcaggagctc cccttgtcca tccagggagg
    16801 gattggggat gcgccttccc actcagacgg gagatggcag tcgtgacctg gggaatcgta
    16861 cccgctgcgg ggcgcggcac acgcattcag ccgctcgcgt tctcgaagga attgctcccc
    16921 gtcgggagcc gcctgtctgg cgccggtgag cgcccgtgtg cggcctctga gtatctgctc
    16981 gaccggatgc tgctcgcggg cgccaaccgg atctgcttcg tgatctcccc gggcaagtgg
    17041 gacatcatgc aatattatgg tgccgagtac ggcggcgccg ccatcgccta cgtgatgcag
    17101 ccccagccgg ccggactgtg cgatgccgtc ttccgcgcag agccggtgat ccgcgcggat
    17161 gagggtgtca tcatcggcct gcccgacacg atctggttcc ccgagaccgc gctcgccggt
    17221 ctgccggagg atcagctcgc attcctgctg tttcccgtcg atcggcccga gttcttcgat
    17281 gccgtgatac tcgacgggtc cgggcgggtc ctcgagatcc aggtgaagca ggcaaacgcc
    17341 cgttcaaact ggatctgggg cgcattcaga atgccgggcc acgtcttcat cgagctcaag
    17401 cgatactggc tcgcgcgcaa ccgctccgac gagtatttcg gcacgctcgt caacgcctgg
    17461 ctggcagaag gcggcaccgc ggtcggcatc aaggcggggc aggcttatgt ggatgtcggc
    17521 accctcaacg gttatcgcgc ggcatcggca ctgctcaccg agcaagcctc ctcctcggct
    17581 gttgcggacg gggaggagcg gctcaagcgg ccgactgccc cgccctccgg tggcatgctg
    17641 catgaggtga gccggtgacc acagtcgaga cggccccccg ttcacgagaa gaaatcgcgc
    17701 gccgcatcgc cgagctcggg ccgtggttcc acaatctcga gcttgccggg atcaagacgg
    17761 ccccggacca cttccttggc gactatccgg ccataaaggc gcgcacgttc tgggatgacg
    17821 tcgcggccag gctgcccggg cgcagcgtgc tcgacatcgg ctgcaatgcc ggcttctacg
    17881 ctattgctgc caagcgtgcc ggtgcatcca gggtggtcgg catcgacgcc gatccggact
    17941 atctcgcgca ggcgcggttc gctgccgagg tctgcgagct cgacatcgat taccagcagc
    18001 tctcggtcta cgacgtgggc gcactcggcg agcggttcga cgtcgttctg ttcctgggcg
    18061 ttctctatca cctgcgtcac ccgctgctgg ccctcgacct cattcacgag cacgtggccc
    18121 aggatcttct ggtttaccaa tcgatgcagc gcggcagtcc ccatgtggcc gaggtcgcaa
    18181 gcgactacga tttctgggaa gcggagcact tcgaagagcc cggctacccc aagctccact
    18241 tcatcgagca ttcctactcg ggtgatccga ccaactggtg ggcgccgaac cgcgcctgct
    18301 ccaaggcgat gctgcgcagc gcgggcttcg ccatattagc caatcccgag gaggaggttt
    18361 tcatttgcca gcgcgtagcc gcgcctgagg gcgccggcgc cgtctatccg tcccgggggc
    18421 atgaatgatc gaagcggcca tgatctggaa cgagccaaac aacaagtccc attgggaccc
    18481 ggagctcgat cccgaatggg cgcgatttgc cgaaatggca aatctcgccg gaaaagcgat
    18541 ccgccgcgaa aatccgaacc tgcttcgcgt gctcggcggc atttcaccca tcgacccgat
    18601 cttcatccgc aacatgtctc aacgtggcgt gctcgacaat gtcgatgtcg tcgccgtgca
    18661 cgggttcccg ctcgactgga atctgtggca aatccacgaa tggccggaca agctcgaaga
    18721 gatccgcgcc gaaaccgaat tgccggtctg ggtgacggaa gtcggtgttt cgaccttcgg
    18781 cgctgaggag gtgcaggtct gggggctgca gcgcacggcc gaactgctta tcggccgcac
    18841 gccgcgcatc cattggtaca gcctttacga tctgccgcgc acgtgggaag caacgacacg
    18901 gcacaaggaa gcggagggct cctcatatta ccggcacttc cacatggggc tgttgcgaga
    18961 ggacggcaca ccgaagcagg cgctcgacaa atttgcccgc tacacgcccg acatcgggat
    19021 ctgccagtgg ttccatttcg aggaccatcg gctggacgag gcggtcgcct ggatgaagcg
    19081 gctgggggtc cggtatctca ggacgggcct ttcctgggcc gacagcttcc gcccgaatgc
    19141 gctgcaatgg ttcgaccggc agatggaagc actcgcagat ttcgacgtga cgctgacctt
    19201 ctgcttcacc ccggagcatc gcgggattgc gccgcatcac accagcccgc cgctcgtcgc
    19261 cgaggagttc gccgaattct gcgccagcat ggtgcggcgg tattgtcccg ggcatcgggt
    19321 gccagcgaac caacttatga ctggcgcgta atctgctggg taacgaattc ggtggaattg
    19381 caacacgcgc gatggaaaca cgcggccggc catctcaaaa atctgaagag cgaatttctt
    19441 ttctgctcga ccagaaaaga agtcgcgtcg ctgccttcga cgtcggtgtc gttttcgcgc
    19501 atcccgatga cgagacgatc ggctgcggcg cccagctcgc gcgctggtcg ggcgcatgtc
    19561 tcgtactggt tacggacggt gccccccgga acctgaacga cgctcgcgca gcaggatttt
    19621 cgaccgccgc cgaatacgcg gaagcacggc tcgaggaact gaaaaatgcg ctgagaaccg
    19681 caggcgtgcc aagcgattcc ctcgtcagat tcgggatccc tgatcagcag gccgcgcgct
    19741 ggctggtcga gctgacgaac cgattggtcg gcatcgtcgt cacacgcggg ctggacgtcc
    19801 ttttcaccca cgcctgcgaa ggaggacatc cggatcatga cgcggtggcc ttcgcggtac
    19861 acgctgccgc gagactgctg gaaagccgag gtcgccccgt cactatcgtc gaaatgccgt
    19921 attacagatg cggcgctaac ggaatggatc gccagcaatt caattcggca ccggacgtag
    19981 aggagttcat aatccacctg acgcccgatc agcagatctt gaagagacaa atggtgtctg
    20041 cacaccggac gcagcaaata gccctggcgc cgtttgatct gtcgatcgag cgttttcgga
    20101 tggctccggc ttacaatttc tcggagttgc ccagcgaagg ccggcttctc tatgagcact
    20161 acgattgggg cctcaccggg cgcgaatggt tgccgctggc acggtcggcc ctggcggagt
    20221 tgggcttgga caagcgctcg tgctgaggat cttgcaggtc gcttattcac tggcccccgt
    20281 cggccccgat gcggtcgggg ggagcgagca agtgctttat catctcgaac gtggcctcgc
    20341 cgcgcggggt ttcgagacgc tggttatcgc cagcgaggac tcgcaggttt cgggaaagct
    20401 cattcccttg ccgcgcgcga gcctgccata tgacgaaccg agaattgcgg cgatgcgggc
    20461 gcgacaggcg aacgccattc gggcggcact atcgcgtcat cgcatcgatc tcgtccacat
    20521 gcacgggttc gatttcgact gctacctgcc gcctgcaggt gtgcctgtgc tcgcgacctt
    20581 gcactgcccg cccgcgtggt acagctcgaa tgcactttac acgtcgaaac cgggtgtctg
    20641 gctcaacgcc gtttcgcgat atcagtttga ggatctttct cccgatgacc agcttctggg
    20701 attcgtcgaa aacggagttc ccattgagga tcttggcaac ctgcaccatg cccgccggcg
    20761 ctttgcgctc atgctcgcgc ggatatcgcc cgaaaaaggc attcgcgagg cactcgaggc
    20821 ggcacggagc gcggacgtac cactcctgat tgctggcagc ctgtacccct accccgacca
    20881 ccagcgctat ttcacggaag agatagcacc gcttctcgac cgaaagaggc gttatctcgg
    20941 cccggtcgat ttcgcgcgca aacggcatct tcttgcagcc gcgcaatgcg tcgtcattcc
    21001 gagcatcgag cctgagacga gctcgctggt tctccgcgaa gcgttcgccg ccgggacagc
    21061 cgtgattgcg ttccggcagg gtgcgctggc ggaggtcata gatcacggcg tgaccggcct
    21121 attggtggac agcgtcgccg aaatggcagc ggcacttctg acgacgtcag ccctggatcg
    21181 agacgcatgc cgcaaaattg cacgcgagcg tttctcactg gagcggatga tcgatggata
    21241 catccaactc tatcaggagg tccttcgccg aagcgcaagt cagattgcgg actttgagat
    21301 cgcgcggtga gtgccgtact cgacattcag acgatcagcg acgccggtga gcttgccgcg
    21361 ctggctccgc aatggtggga gctctgggag catgctcccg cggtgacgcc ct
//
LOCUS       DASVDU010000228        38603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_631606, whole genome shotgun sequence.
ACCESSION   DASVDU010000228 DASVDU010000000
VERSION     DASVDU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 38603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38603
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631606"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1088)
                     /locus_tag="VF226_14550"
     CDS             complement(123..1088)
                     /locus_tag="VF226_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEX7075256.1"
                     /translation="MASGGRHDQGGSRLDDAARAGWLYYVAGNTQDQIAAKLGVSRQS
                     AQRLVSLAVSEGLVKVRIDHPIAGCLELGRRLQGRFALDYVEVVPSDPASRSTTIGVA
                     QAAAAEIERRLAPSEPIVMAIGTGRTLKAAIEQLAPMECPQHKIVSLTGNIAPDGSAA
                     FYNVIFTLADTVKARSFPMPLPVISSSPEERELLHNQPMIKSTLALAAKANVTFVGIG
                     DLGPEAPLFVDGFITPAELKALQKAGAVGEIVGWAYDREGRLVEGITNERVASAPIPS
                     RENSLVIAAAMGERKLPGIFAALKRRLVNGLITDERTAAALLETA"
     gene            1515..2213
                     /locus_tag="VF226_14555"
     CDS             1515..2213
                     /locus_tag="VF226_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7075257.1"
                     /translation="MTVAALESRTLASQSAIAGRRDLSAFMRQLCARTGADAYVLLDA
                     TPGVGPYDPPILASNWTYDAIQSTGVDTIRHIGESSVSVYVGTEPHGWHPLALAVLLG
                     REEIANLVEFGHEEIVALRLHIAGKRYHLLLSSARTGALDCTAIFIEHMRCGYALAQF
                     AGLISPDGDSPLSERERECLSWVSQGKTTEEIAVILDVSSNTINSYIAHAIHKLAASN
                     RAMAVASAIRRNLI"
     gene            2217..2963
                     /locus_tag="VF226_14560"
     CDS             2217..2963
                     /locus_tag="VF226_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7075258.1"
                     /translation="MTAKSNTDDEETTMTAVPRCGDVQKNLKTLPDIACILRGMTAEI
                     GAEEFVLCLAGRREAASPLIRVMDSAYPGISPSANRLVAHLPGWLLVKASASLIPLWW
                     KGCDASAVVGLEAAPWAERVELASVVPGVILPLCTERGQKGLLLFSGGAISVDPDRLT
                     AIQARCFGLFAVAVDVWPRRNRAGPPISKRELECLKLTANGHTSEEIGRLLGLSVHTT
                     NQYLTSTTQKLNAVNRVHAVAKAIREGLIN"
     gene            complement(2973..3413)
                     /locus_tag="VF226_14565"
     CDS             complement(2973..3413)
                     /locus_tag="VF226_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075259.1"
                     /translation="MNRQPHSAAREIIIAEAIREVANELRMIDVADYVAFIRLESMAS
                     IADIVESAAELYFMPGTLRLGHGCEAHVGWTGEPRIVLDLELRPPGVSVYFTLTLMAE
                     HATVEVNYVSFDEALPDPEANSAFLAKALNEARIRKAVDSCTGA"
     gene            complement(3410..4504)
                     /gene="flhB"
                     /locus_tag="VF226_14570"
     CDS             complement(3410..4504)
                     /gene="flhB"
                     /locus_tag="VF226_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX7075260.1"
                     /translation="MAEEQDKESKTEEATEKKIRDTIEKGQLPFSKELPVFASFLGIL
                     VFVIFFARDSAMRLASFLSIFMERPEEWVLETQHDAIALYRIVFLEIAKVLGTVMLLL
                     VVAGIAASILQNTPRFVVERVRPKASNISLRQGWRRIFGAKGMVEFLKSIGKLLVAGS
                     FLALALREAQSSLLAGMFTPPAEFGGIVIRDITIKMLMTVTLIMAVIASADLLWSRFH
                     WRQDLRMTRQELKDELKQTDGDPIVKARLRSLARDRARHRMMAAVPQATLVIANPTHF
                     AIALRYIRERDSAPVVVAKGQDLIALRIREIAQTHGVPVFEEPALARSMYKQVSVDSM
                     IPPQFYRAVAELIRLVYRAGAQRQPMRSSA"
     gene            complement(4517..5530)
                     /locus_tag="VF226_14575"
     CDS             complement(4517..5530)
                     /locus_tag="VF226_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX7075261.1"
                     /translation="MTGKLNLTRAQKAAAILVAMGKPAAGRLLKFFKQEELKTLVEAA
                     RELRTIPQAELNRIVGEFEAEFAEGAGLLDSADKMNMLLNESLSPEELGLLMGQNVPR
                     VDSQSTVWGDMERLDPQRLADLLSNEHPQTIAYVLSNLSPEPAARILVTLEKPIRTEV
                     LRRLISTSQIKPKAKQIVERQLRNLLQAEASGKDVSAGQAKVASLLNELDKTQIDEVM
                     RDLEESGTSDVASIRAQIFSFEEIPLLPHKARVALFDGFDTDVVTLALRGAASELKEA
                     VLSALGPRSRRMIESELSINVDGLQAEEVTRARKRIASAAIQLARRGVFVLPSAQKQE
                     EAA"
     gene            complement(5574..5960)
                     /gene="fliN"
                     /locus_tag="VF226_14580"
     CDS             complement(5574..5960)
                     /gene="fliN"
                     /locus_tag="VF226_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX7075262.1"
                     /translation="MNEPNPATEDLEEEQLNKAIEELRGVLHDGKEGGAQADGRFGGN
                     AAVIMGIPVEVQIVLGRAEMTVSELMALQKGSTVSLDRRIGEPVEVVVNGRRVARGEI
                     TVLEHDPSRFGIRLTEVAGTEHEKPD"
     gene            complement(6003..6938)
                     /locus_tag="VF226_14585"
     CDS             complement(6003..6938)
                     /locus_tag="VF226_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX7075263.1"
                     /translation="MSESDDPEIMRRLVVERLIGATDDPDRVIAAARKSAARAVPLVL
                     EKLSEQLSFELDVELKSVELIRLADAIPAPDSGEAVAVAGSATSPDALTLQIDSRAIS
                     LFLDAYLGGDPNHPPIPIERELSAIELDVTHQVLEIFAQAINGHGARALGLRFPLAAP
                     VAGEGLKRLAVRDGPGVRMVFSVASACGDGEVRALMPQRVLLQERSPTGNSTDETANQ
                     AQWRQRFNEELMRSAVAFEAKVPMPPMTLGDVAAFRVGQLIELPVNARSETRLSVRGR
                     PVFICEFGKLGQNYTVRIKDRFDAGQEFIAGLAAG"
     gene            complement(6975..7856)
                     /gene="motA"
                     /locus_tag="VF226_14590"
     CDS             complement(6975..7856)
                     /gene="motA"
                     /locus_tag="VF226_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX7075264.1"
                     /translation="MGIIIGLVVTVGCVLGGFMAMGGHIHVLLQPWEAVVICGAAFGT
                     FLVANPMSTVKDAARACMEAFKHDVPTEKEYLETLGVLHSLMRELRTKTRTEVEAHID
                     NPEESAIFQRFPTVLNNRDLTHFICDYCRIIIIGNARSFEIEALMDEEIQTIRRDKLK
                     SYHAMISVAEGLPALGIVAAVLGVIKAMGALDQSPEILGALIGAALVGTFLGIFMSYA
                     VVGPIAAKVKVVREKKNRLYVIVKQTLLAYMNGSLPQVALEFGRKTISSKDRPTIDEV
                     EQSTLNPGGDEAQSRAA"
     gene            8008..9126
                     /locus_tag="VF226_14595"
     CDS             8008..9126
                     /locus_tag="VF226_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEX7075265.1"
                     /translation="MTTFASYQLIVRDINKSISRVENQPLVRRETEYYLANIGKVKSI
                     EEFVSDDRLFRYAMKAHGLEDMAYAKAFMVKALKEGVSDPDSFANRLTDKRYAEFVKA
                     YNFAALGEDATIFNPAQQGTADRYLLAAVQAGVDPDDELLNQQIDYYMENIVKVRSID
                     EFLADDALYSFALKAFGLADRLGDKDFIRELLEGGVDDPDSLANRQKDKRYAEFVAAF
                     NFARYGPDATTINPALQPTVDAYLRQTLEENAGEQNEGVRLALYFERKAPDIDSFYDV
                     LADRALAQVVRTALGLPEAIARTDIDRQVAMMEARMDIGDFQDPEKLDDFLKRFTSLW
                     EITNPSTSRESSVSVLFGQPAEFGISTDLLLTMAQLKR"
     gene            9136..9855
                     /gene="flgF"
                     /locus_tag="VF226_14600"
     CDS             9136..9855
                     /gene="flgF"
                     /locus_tag="VF226_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX7075266.1"
                     /translation="MQNSLYVALSSQIALQRRLDTIADNVANAGTVGFKATGIEFSDV
                     VAGFGDDAVSFTSTGGTYIGSQPGTLRETGNPFDFAVQGDAWFAIQTPAGVVMTRDGR
                     FTMLDTGELVTLNGYPVLDPGGTPIVLNPRGGPPEAASDGTLRQNGELLGAIGLYEFN
                     PGENFIRFENSGIIPQGEPQPIVDRPEIGVAQGFIEQSNVDPVKEMTNLIQVQRTFEQ
                     VTALMRDSETALEDAIKTLGS"
     gene            9879..11282
                     /gene="fliI"
                     /locus_tag="VF226_14605"
     CDS             9879..11282
                     /gene="fliI"
                     /locus_tag="VF226_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEX7075267.1"
                     /translation="METAETNIGREGALGAANALSALEEAYRRFIDPRFILKRGGRIA
                     EISPTHYRIRGLSDSARLGDIVEYGPRSRPRTAEVVHIAREEVLVAPFENDGTAGLDE
                     AVFNRGPVHITPHASWRGRVIDALGRPIDDRSRSLNYEPEHSDDRRTPPALRRQRVAE
                     RFLTGVRVIDIFTPLCHGQRLGIFAGSGVGKSTLLAMLARAEAFDTVVVALIGERGRE
                     VREFLEDTLGPDGMAKTVAVVATSDESPLMRRRAADTAMRVAESFRERGERVLLILDS
                     ITRFAHALREVAIGVGEPPVARGYPASVFTALPKLLERAGPGETGSGSITAILSVLVD
                     GDDHNDPVADCVRGILDGHVVLDRGIAEQGRYPPVNPLASISRLADRAWTGEQKVLVR
                     QLRAMISRFEDTRDIRLLGAYKPGADAELDQAVRQVPAIYEALRQSPGERPSLDPFAD
                     LARALRAMENNDAGHVG"
     gene            11263..11901
                     /locus_tag="VF226_14610"
     CDS             11263..11901
                     /locus_tag="VF226_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075268.1"
                     /translation="MPDTSAEPESSKKVHVEEHPNLPGMQGDLAGSPPSRPRIAASFA
                     WFGRTLGRGLTSDVGLATAGVMLAAICALFPWYIFLHQDQFGIRPLEFEGRAIQSSPS
                     PAGQPRPIGTHVALEEIEALGLDAIPTGTVHRDAEPVSAPAAEQPYPGEEAVFELVYV
                     AAGRAMIRDGSGFWVVQPGSRLPDGSRLVSIEKRDGNWVLVTSRKTVISLTR"
     gene            11994..12374
                     /gene="flgB"
                     /locus_tag="VF226_14615"
     CDS             11994..12374
                     /gene="flgB"
                     /locus_tag="VF226_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HEX7075269.1"
                     /translation="MQPVNLFDLAAQQARWLSVRQAAVAGNIANVNTPGYRTVVVEPF
                     DEVLDNRRVSLRATHPDHLTTGATGNAFDLRRAEPDTALLPSENSVVLEEELVKQSEI
                     RRAFELNTAIVKAFHSMLMMVTRS"
     gene            12380..12799
                     /gene="flgC"
                     /locus_tag="VF226_14620"
     CDS             12380..12799
                     /gene="flgC"
                     /locus_tag="VF226_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEX7075270.1"
                     /translation="MDPLVGALKVAASGLGAQSERMRVVSENMANAYSTSNVPGGDPY
                     RRKTITFAAHLDEATGGSLVEVESIGRDPSAFPVKYDPGAEAADEFGYVKMPNVNILI
                     EMADMREANRGYEANLQVIKQARELVSMTIGLLRSQQ"
     gene            12796..13110
                     /locus_tag="VF226_14625"
     CDS             12796..13110
                     /locus_tag="VF226_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEX7075271.1"
                     /translation="MISALGSISPVTVSGLGNDRPATVSVAPPGGGEEDFGSVLGETL
                     GRTVAQLENAEKLSIQALRGDAEIREVVDAVMAAEQTLQAAIAIRDKIVTAYLEISRM
                     SI"
     gene            13136..13924
                     /gene="flgG"
                     /locus_tag="VF226_14630"
     CDS             13136..13924
                     /gene="flgG"
                     /locus_tag="VF226_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEX7075272.1"
                     /translation="MKALAIAATGMSAQQLNLEVIANNIANINTTGFKRARAEFSDLL
                     YQVERLQGVPTRADQGIIPEGASVGLGVKTVAVRNLHQQGSLTNTGNKFDLALVGKGW
                     FQIEGADGEPLYTRAGTFNTSATGQLVTVDGFPVQPAIVVPEDAVDVIINKSGQVFAR
                     IDGQTDLQELGQLTLANFSNEAGLEPIGNNLFRETVASGPANVGVPGDPGFAEVEQGY
                     LEDSNVDPVKEITELISAQRAYEMNSKVITAADEMAATITQSMR"
     gene            13933..14394
                     /gene="flgA"
                     /locus_tag="VF226_14635"
     CDS             13933..14394
                     /gene="flgA"
                     /locus_tag="VF226_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEX7075273.1"
                     /translation="MRRVTAPCLLLFVLLATAGAPASAQEMAVVSTRVIYPGQTIQAD
                     ALKDIVLKRPPRGISAIVRSSDEIVGKVARRTLLPGRLIPEGSVREPYLVETGSPVTV
                     IFRSGALSISLIAVPLESGAAGDVVKLRNMDSGAVFSGVVLSDGTVRVGTT"
     gene            14391..15494
                     /locus_tag="VF226_14640"
     CDS             14391..15494
                     /locus_tag="VF226_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEX7075274.1"
                     /translation="MILRALIGSFLALAAAFPAAASSRIKDIATLEAARDNQLVGYGL
                     VIGLQGSGDSLRNSPFTEQSMRAMLQNLGIATEGGRMRAKNVAAVIVTANLPPFVQSG
                     ARIDVNVSSLGDATSLAGGTLVMTPLRAADGEIYAVAQGPVIVSGFNAEGEAQKLTQG
                     VPTAGRVPNGAIIERKVEAAFSHIDTLTLQLRNPDFSTAVRVADAINSYATRRFGKKL
                     AVERDARTVVVHKPANISTARFYAELENLVVKPDTPARVVVDERTGTVVIGQDVRISR
                     VAISYGTLTVRITEMPRVVQPNPFSRGETAVEPFTSIEADQPDARVALLDGPNLETLV
                     SGLNRLGVKPDGIIAILQGIKSAGALQAELVLQ"
     gene            15567..16034
                     /locus_tag="VF226_14645"
     CDS             15567..16034
                     /locus_tag="VF226_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HEX7075275.1"
                     /translation="MFALMLAQAIPPPALAAEAGSSQPGGEIQRFCSNIADAARDRRY
                     ALQTKRLENLKAEIDKRVEALEKKRAEYEDWLRRREEFLAKAEDNVVAIYSNMTPDAA
                     AEKLAALNVELAAAIVMKLDPRKAGVILNEMNSKVAAALTGVMADAARRNDPS"
     gene            16031..16735
                     /gene="flgH"
                     /locus_tag="VF226_14650"
     CDS             16031..16735
                     /gene="flgH"
                     /locus_tag="VF226_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEX7075276.1"
                     /translation="MMRLLPVAASCIALQACGANFQEIGVPPALSPVGSGIHPAALSD
                     MQYPQAPSRPVTRFSLWDDKQSQFYTDARAINIGDILTVEIRMNDRAEFENESKRKRD
                     SVRTLGLAGSYSAGSSGSSASLDGQIDSVTSSAGSGSTERSEEIKLRVAAVVTGVLPN
                     GNLRISGSQEVRVNAELRVLTIEGIVRPSDIGSDNTVSYDRIAEARISYGGRGRLTEV
                     QQPPYGQQILDQALPF"
     gene            16753..17259
                     /locus_tag="VF226_14655"
     CDS             16753..17259
                     /locus_tag="VF226_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEX7075277.1"
                     /translation="MAIAEKGEAQRTGPPLVVQLVLFAALTFMAAGLGWLTGSNLIPL
                     AAEKSKPGTITSDREETTSPADEPAEGEAVEPNLYTLEPITTNLADPRDIWVRLEVSL
                     AFEGPADAGLAQEIHQDILAYLRTVKLEQIDTASGFHHLKSDLEERAAIRSNGRVTDI
                     FIRTLLFE"
     gene            17256..17993
                     /gene="fliP"
                     /locus_tag="VF226_14660"
     CDS             17256..17993
                     /gene="fliP"
                     /locus_tag="VF226_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860957.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEX7075278.1"
                     /translation="MKRLCPTAAILLMSGATASAQTFDLGQLASQADGTTVGYIIQLF
                     GLLTVLSIAPGILIMVTSFTRFVIAFSILRSGIGLPTTPANLILISLALFMTFYVMAP
                     AFDQAWNDGVRPLMNNEITEEQAVKRISDPFRTFMMNNVREKDYALFADLARARGEEN
                     VAVEQGDLRILIPAFMISELRRGFEIGFLIVLPFLVIDLIVATITMSMGMMMLPPTVV
                     SLPFKILFFVLIDGWNLLVGSLVRSFT"
     gene            complement(18095..18952)
                     /locus_tag="VF226_14665"
     CDS             complement(18095..18952)
                     /locus_tag="VF226_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7075279.1"
                     /translation="MTGAINEDAAAGFDPLRPKLMRVAYRMLGSVADAEDMVQEAFIR
                     WMRTDRDEVRDPEAFLRRTVTRLCLDQLKSARRQRETYIGPWLPDPVVEEEEEEDVTL
                     PLMLALERLSPLERAAFLLHDVFGLGFEEVAATIQRGPAACRQLAARARAHVREARPR
                     FQVEKQRGLELAKAFFVASRSGDMKALGAMLAADVSIHADGGGKRPAAMKPIVGFDAV
                     MKIQERLAALFQENGSKLIRTGFVNGLPGFITLEADGEIQTTALEIEDDKIAVIYVVR
                     NPEKLRHLH"
     gene            complement(18952..19422)
                     /locus_tag="VF226_14670"
     CDS             complement(18952..19422)
                     /locus_tag="VF226_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7075280.1"
                     /translation="MTAKLDPFAAAPSLMTEWRRLSLAVDSSLEPSLSELVKIRASQI
                     NGCANCLNMHTVFARENGETEQRIYLLSAWREAPCYTDRERAALAWTEALTRLSEGHE
                     HESAYEALKAHFTEEEQVKLTLLINVINGWNRLAVGFGSWVDPAAVKAAAKAAA"
     gene            19683..20090
                     /locus_tag="VF226_14675"
     CDS             19683..20090
                     /locus_tag="VF226_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7075281.1"
                     /translation="MAFDRIWALAPQWLSILRIMSALLFLEHGTQKLFNFPPAPEPME
                     LSGLFLLQGLIEVVGGILLAIGLFTRPVAFILSGNMAVAYFMAHAPMNFFPMFNQGDA
                     AILYCFVFLYFFFAGGGAWSVDAVRGGSGLAKP"
     gene            complement(20174..20875)
                     /locus_tag="VF226_14680"
     CDS             complement(20174..20875)
                     /locus_tag="VF226_14680"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7075282.1"
                     /translation="MKNEDRDDVPQESGAAAGIANRPETGAPALDFLGKRLRQEREAR
                     GLSLRELARRIGVSPSLVSQIERGLVTPSVSTLWSIASELDLTIDELFNASERSTAPP
                     PPTGAKAHGSEGPVQRRHDRKRIRLAGGVVWERLTAQPDETVDFLHVVYEVGAESCPA
                     DSLFRHGGKEYAYIISGRLGLQVGFETYELGPGDSVSFNARLPHRLWAIGDTPAVAIW
                     AVINRTDDSRGNPQG"
     gene            20988..21887
                     /locus_tag="VF226_14685"
     CDS             20988..21887
                     /locus_tag="VF226_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7075283.1"
                     /translation="MVHYVARAADIKLFPAYPGKSLGYRRSSVVDRSVGSVHTALGLC
                     ELDEGGHIDVHVHSFEEQFYITEGKPTLVLDGRGYALDPGACGVIPVGTPHAWLGPAG
                     GTAKWIDFMSPIPRENGPRDTFFLGPAGDVKVAPVDIRDPRTRHFFRMADDDIDVEKL
                     KVGSRVDAPKVSASMATALLAYSGIAVKMLVDQRLSAALGTMFMVEYQPGGVAHPHDH
                     PLEEAYVILHGEVEAVADDKTYTLKTGDVLWTGVGCIHAFYNRTNTTVRWLETQSPQP
                     PARHSYRFSRDWEYLEEKLAEQH"
     gene            21889..22578
                     /locus_tag="VF226_14690"
     CDS             21889..22578
                     /locus_tag="VF226_14690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075284.1"
                     /translation="MANNAIIIGGTQGIGLEIAQQMVQRGDQVIITGRDKARTEKVAA
                     ELGHGVKALAVDLSKPHDIAAALADVPQLSHVVITAILRDANSARNYDVDRALNLVTL
                     KLVGYTEAIHALLPKLNNDASIVLFGGLAKERPYPGSTTVTTVNGGVATMVHTLAVEL
                     APIRVNAIHPGIIGDSPAWSGKNLDAVVARTPTKRLATMRDVASAALFLLDNNSINGV
                     NLNIDNGWLLT"
     gene            22604..23869
                     /locus_tag="VF226_14695"
     CDS             22604..23869
                     /locus_tag="VF226_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7075285.1"
                     /translation="MKITRRRFTKLGAAASIGLAAPAVWTKGFAQSGKIRVGCSASLT
                     GPLSSTKNMVIGYELWRDDVNKAGGLLGKEVELIVYDDQSSPSNVPAIYSKLVDVDQV
                     DFLFTPYGANLTATIMPFAKQRDRFIVGILTLASNDHLRHDKFFQAAPWGPDGATNWA
                     RGFFDIAQRDGVKKMAILAADTEFSKTAAEGGRRVCEETGMEVVDFQMYPPTNRDFSS
                     ILRNIRAAEPEAVFVCSYPADSTAIARGIQEIGLGDSVRLFGGGMVGPQYASLLENLG
                     PALNGIVNFHLYVPEPTLKFEGIDGFLARYVPIAKERQVDPLGFYIPPFTYAAGQLIS
                     KAVEATGSIDQAEVSQWLHENSVDTVVGQITFDEIGNWTERRVLMVQFQGVDDSGIEQ
                     FRQAGKQVVIDPTAYASGDLIPFAEARKA"
     gene            23931..24809
                     /locus_tag="VF226_14700"
     CDS             23931..24809
                     /locus_tag="VF226_14700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075286.1"
                     /translation="MVVLDLVLNALATGVMLGSIYALLALGLAITFGILHIPNVAHPA
                     LVIAGAYAVVTSNRFGLDPILAGVLWAVPFYLLGILFYEFYSRSFESRGGGNTLQSLT
                     LFFGASLIVEVGLVVAYGTDFRSVNVDYVGSSLRLGMVAIPYRFLIPALMAPVAVFLL
                     WLYLSRTNIGLAIRAVAHDERAVSVSGINPATIKRHAFGIATALAAIAGAALVITGPI
                     DPFAGRIHIGRVFAIVVLAGMGTIPGTLAAGIIIGIAESLVMSFMSPSWAPGVAFAIL
                     LATLALKPTGLFGAAR"
     gene            24806..25741
                     /locus_tag="VF226_14705"
     CDS             24806..25741
                     /locus_tag="VF226_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075287.1"
                     /translation="MTMARFTMLALLVFVAGLFLPKLVSNPYYFYAGYVVLQYVVIAT
                     GWNILGGYAGYTNFGASAFFGAGAYTAAFLFKALGVSIFVQILGACAVGMLLGLATGY
                     LTLRIQGVYFAIATVALVIVLETLVHNTEYLGGASGMTHLAPPPPAWFGGTTQYVFCI
                     MLGLAVAAVVLARWLEVSWVGRGFRAIRASEPAAEACGVPTLKLKLLACALSGGVMAA
                     AGAPYPTYTFYLDPVSAFSLLIGLNALAMPLIGGTRTWLGPVIGAVLLASVQQLATVT
                     ISSEFNILAVGLVLIAFVVAAPEGLLGIRFRRRAA"
     gene            25738..26472
                     /locus_tag="VF226_14710"
     CDS             25738..26472
                     /locus_tag="VF226_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075288.1"
                     /translation="MTGSLLTVRDVSKSFGPIKALGPVDLDLMPGERLGIIGPNGSGK
                     TTLINCISGFYRPDSGSIVFKGEDITAWPAYKRARIGLSRSFQIPRPFLGMSVFENLL
                     VPMDYCSESENEKEEIERAGQVLESVGLAARRNDPAEMLSQLELRKLELARALAARPK
                     VLIADEAMAGLSEAEIDDVLALLFTLNETGVAVVMIEHIMHAVMRFSQRVICLETGTI
                     IAEGNPDEIVASPQVQKVYFGEQTRR"
     gene            26453..27184
                     /locus_tag="VF226_14715"
     CDS             26453..27184
                     /locus_tag="VF226_14715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075289.1"
                     /translation="MNRLVVEGLHAGYGAVAVLRDISLEVREGELVALLGMNGNGKST
                     LLNCIMGFLRPTSGRIFLEWEGETIELSSSAAHETIRRGIAYVPEGRRLAPNLTVEEN
                     LLLAGGSGHAKAKLKENLDFCYDMFPILNERRRQLSSTLSGGQQQMLAIARALMTSPR
                     IMMIDEPSVGLAPIVVEQVINTIRTLQENTNLTILMAEQSFFQAIEVASRAYVLSHGR
                     IIHTFDQKVDPASSDEIRNAMMGVA"
     gene            complement(27319..27606)
                     /locus_tag="VF226_14720"
     CDS             complement(27319..27606)
                     /locus_tag="VF226_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7075290.1"
                     /translation="MLFVIHALDKPGALETRLANYDAHKAFLSDTSAFGVKIVMSGPL
                     VADDGQTMIGSLLLVEAPDRAAVERFNNADPFKAADIWEKVTITAFIRRQG"
     gene            complement(27656..28519)
                     /locus_tag="VF226_14725"
     CDS             complement(27656..28519)
                     /locus_tag="VF226_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HEX7075291.1"
                     /translation="MRNFNEYTITDAVLERVRNSPDPRIRQISEAVVRHLHALVREVR
                     PTQEEWMAAIQFLTRTGQACDDKRQEFILLSDTLGISMLVDAINHANSHGATETTVLG
                     PFYVESPPEYQLGDNISPGIEGDPLFVSGSVRSTDGTPLAGAVVDIWHSDDDGHYDVQ
                     QLDKVGGLAMRARLRADERGRFHFWTIKPAAYPIPYDGPVGEMLAAQGRHPWRPAHVH
                     FMISAPGHQRLITHIFVAGDQYLDSDVVFGVKDSLICDFVERPAGTAPDGTAMNRSYF
                     ELQYDFTLDRG"
     gene            complement(28529..29431)
                     /locus_tag="VF226_14730"
     CDS             complement(28529..29431)
                     /locus_tag="VF226_14730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020508600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075292.1"
                     /translation="MSGKAAIGWIGLGKLGLPIAARIADAGHAVAGYDIAQERRDAAS
                     AKGIAVVTDPGEAAGKQVVFSCLPDDRALRSAALGDRGILKTMSDDAIYVEISTVSPE
                     VSAEVARVAEEAGIAYLRLPISGNASIAHTGNLTCFASGPKAAFEAVRPVLSTFTRAQ
                     IYLGEGEEARYAKLAVNLMLAVTAVMMGESLALARKGNIGWQDMLNVLADSAAASPMV
                     KYKVDQLARRDFTSTFSCYQMAKDLDLILGAARSANVPMPVTALARETFAAIIGRGMG
                     EDDFIAPVKHIEWLAGMGEPDRKE"
     gene            complement(29535..30602)
                     /locus_tag="VF226_14735"
     CDS             complement(29535..30602)
                     /locus_tag="VF226_14735"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HEX7075293.1"
                     /translation="MKRFVYTSAPARVVFGSGTLSVLGEEIERLGCSRVLVLSTREQS
                     GQARDVAERLGAKAAGVFDGAMMHTPVSVTEEAMKVVSELGVDCTLAVGGGSTIGLGK
                     AIALRTDLPQVCVPTTYAGSEMTPILGETADGVKKTQRTPKVLPEVVVYDVDLTLSLP
                     PALSATSGMNAIAHAVEALYAEDRNPVISLMAEEAVSALTRALPGVVADGSDREARSD
                     ALYGAWLCGICLGSVGMSLHHKLCHVLGGAFDLPHAETHTIVLPHVVAYNASAAGDAM
                     AALSRALGADDPAQALYALAGKLGAPRALREIGMPEEGIDHAADLAIENPYWNPRPIE
                     REAIRRLVSRAWAGERPDPAS"
     gene            complement(31052..31501)
                     /locus_tag="VF226_14740"
     CDS             complement(31052..31501)
                     /locus_tag="VF226_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7075294.1"
                     /translation="MIAETPGEESPSRLIDARIRELDDWRGEMLSRLRALIRQADPEV
                     VETVKWRKPSNMLGVPVWEHAGIICTGETYKDKIKLTFARGALLEDPSGLFNSSLEGN
                     TRRAIDIHEDDEIDEEALKALIHAAVALNMSAQAAAPSSRGRKTSRQ"
     gene            complement(31504..31731)
                     /locus_tag="VF226_14745"
     CDS             complement(31504..31731)
                     /locus_tag="VF226_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075295.1"
                     /translation="MNWNIWIRQFHRWLSIVFTLTVIANFVTMGLGEPPSWVVYSPLL
                     PLFLLLFTGLYMFVLPYATRWRSGRCIGKEA"
     gene            complement(31762..32172)
                     /locus_tag="VF226_14750"
     CDS             complement(31762..32172)
                     /locus_tag="VF226_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX7075296.1"
                     /translation="MREMSAETLSVVVEREFPFPPEKIWRALTQPHLIEEWLMKNDFS
                     PVVGHRFNLSADWGTVDCRVQTAEPNKTLCYTWDTKDLTSVVSWTLTPTDTGTLLRME
                     QSGFRADQQSYYRGAKAGWPRFFAALEQVLEKMS"
     gene            complement(32169..32492)
                     /locus_tag="VF226_14755"
     CDS             complement(32169..32492)
                     /locus_tag="VF226_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7075297.1"
                     /translation="MPQPSDILFRALADPTRRAIFERLCREGDQTVSALTARAGISQP
                     AVSKHLKVLKQAGLVRDRHEGRQTHYSVEPGALSPLIDWTKQMAEFWESRFDRLQDLL
                     KRMDQ"
     gene            complement(33092..33358)
                     /gene="fliQ"
                     /locus_tag="VF226_14760"
     CDS             complement(33092..33358)
                     /gene="fliQ"
                     /locus_tag="VF226_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEX7075298.1"
                     /translation="MNEADALDIVQYAIWTVLVASGPAVIVAMVVGIGIALVQALTQV
                     QEITLTFVPKIVAILLAVAFSGPFVGAQISAFSTVIFQRIQNGF"
     gene            complement(33370..33765)
                     /gene="flgD"
                     /locus_tag="VF226_14765"
     CDS             complement(33370..33765)
                     /gene="flgD"
                     /locus_tag="VF226_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEX7075299.1"
                     /translation="MQVSPTQAAGPSTAAQSAQAPQTVDYQAFLRLLIEQMKNQDPTN
                     PMDSTDYMAQLASFSQVEQAVRTNAKLDQLLQSSTLSQAGNLIGREITSSDGEITGKV
                     AEVRLVEDGLVAILETGEEVAVGPGIVIR"
     gene            complement(33771..34208)
                     /gene="flbT"
                     /locus_tag="VF226_14770"
     CDS             complement(33771..34208)
                     /gene="flbT"
                     /locus_tag="VF226_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEX7075300.1"
                     /translation="MTNTLKISLKANEKIYINGAVIRPDRKVSLEFLNDVQFLLEQHV
                     LQVEDADTPLRQLYFIVQVMLMQPDDTEEARAMFRETLTRLLGCFTDQRILASLKDLD
                     RLVGQGQIYEALKLIRRLYPLEAEIMNGERGRNEPPRAIAVGE"
     gene            complement(34205..34552)
                     /gene="flaF"
                     /locus_tag="VF226_14775"
     CDS             complement(34205..34552)
                     /gene="flaF"
                     /locus_tag="VF226_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX7075301.1"
                     /translation="MYQFSYSEIQTDSMADAKDRERQVLSRSIELLIAAREKGSQSRE
                     AVEAIHFLNRVWTVFVEDLGNPDNALPKELRANLISIGLWLLREAEEVRQGRSDNFDG
                     LIEVSQIIRDGMQ"
     gene            complement(34580..35632)
                     /locus_tag="VF226_14780"
     CDS             complement(34580..35632)
                     /locus_tag="VF226_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated family protein"
                     /protein_id="HEX7075302.1"
                     /translation="MKTSLISTHSVAQVMRYQLMRMQAELAEKRQEAVTGRVTDPGVK
                     LGAGVSRTVSMDRDIERLSGLVDSNSLAANRLAATQAALNQITDNAQDLLSALTAAAS
                     GSVDTDVVRHEAVRTLEAVTGILNTSFNGEYLFAGINTDAKPIKPFTDNPPSDSKAAF
                     DAAFQARFGFAQSDPAAASITAAEITSFLDNEIAAQFQGANWTDNWSDAADQPIVSRI
                     TLNETAETSVTANEEGFKKLLMAAVAISDLLKAPLSESARDALIQRAAEIVGEGIADM
                     AGLQGRTGIAENRVSDATDRISSQIDLFKSFINDLEGVDPFEAATRVNELMTQIEISY
                     TLTSRIQQLSLVRYLT"
     gene            complement(35643..37106)
                     /gene="flgK"
                     /locus_tag="VF226_14785"
     CDS             complement(35643..37106)
                     /gene="flgK"
                     /locus_tag="VF226_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX7075303.1"
                     /translation="MSLTSALSIAQSALFNTSRQTSIVSRNLSEASNPDYARRSAVLA
                     SMAPGARVAEIRRATNEVLFRQNLQAISSWQGQKMVSAGLDSLQLDVNGVDNANSAAV
                     MLGKLQEALQLYAASPSNSTLAESAVEAARQMVRSLNQGSAAIQSFRRDTDQQIAASV
                     DKLNGLLADFKEVNNEIISGTRAGRDVSDALDRRDALLKKIAEYVPVSTIKRADNDVM
                     LTTTAGAVLFETVPRPVTFEPTAVFSAGKGGNSVYIDGVALKGGSGGNTTASGSIAAQ
                     LQLRDEVAPAMQRQIDEIARGLIAVFAETDPSGTQPDAAGLFTWSGGPAIPADGTLVD
                     GLGAEISINPAMDSSQGGDPMRLRDGGANGAAYVHNGPPAAASYSELLRTYGEKLDEP
                     MNFDPAAGLEVSASLGTYTSAAVGWLENLRQDAAYGNEAKGALSTRTATALSNATGVN
                     VDEEMSLLLDLEHSYEASARMIRVVDEMLAALLAATG"
     gene            complement(37137..38402)
                     /locus_tag="VF226_14790"
     CDS             complement(37137..38402)
                     /locus_tag="VF226_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX7075304.1"
                     /translation="MSLYGMMRTGVSGMSAQANRLSTVADNIANSSTSGYKRSSTEFS
                     SLVVPSTAGSYNSGGVTTAVRTAISSQGVLQYTTSTTDLAISGSGFFIVQDSSGQPFL
                     TRAGSFVPDGQGKLVNAAGYTLMGYSYANGIPAATANGYDGLEPVVIGQSALTASPSA
                     TGYLSANLPEDAAVVTADSPSDNSATSQYSAKTSLVVYDNTGGQKLLDVYFTKTGANR
                     WDVAVFDRDEATPGGPFPYGTPGSPPLGTAELEFDSSNGKLAGTSDTNVAISVPGGQT
                     LTLDLSAMSELSADFTVFGADVDGNPPSAIDRVEIAGDGTVYAQYDDGSFRALYRVPL
                     ADVPSPDNLQKLSGNVFAESASSGGVRIGFAGEGGMGQMVSGALENSNVDIAQELTDM
                     IQAQRNYTANSKVFQTGSELMDLLVNLKR"
     gene            complement(38514..>38603)
                     /locus_tag="VF226_14795"
     CDS             complement(38514..>38603)
                     /locus_tag="VF226_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEX7075305.1"
                     /translation="ISKLRKKLRERLGFDPIESKRYLGYRLIA"
BASE COUNT         7172 a        12210 c        11795 g         7426 t
ORIGIN      
        1 attcgcaatc tcaggacaaa gtcaatccgg attcgttgct gcactgcagt gtagatccgt
       61 acctgctgga aatgcgtgtg gctccccgcc agccggcacg ggcagcctct gcgcaaggac
      121 gatcaggccg tttcgaggag ggcggccgct gtgcgctcgt cggtaatcag gccgttgacc
      181 agccgtcgct tcagcgcagc gaagatgccg ggcagcttgc gctcccccat cgctgctgca
      241 atgaccagcg agttctcgcg cgagggaatc ggcgcgctcg ccacccgctc gttggtgatg
      301 ccctctacaa ggcgcccttc ccgatcgtat gcccacccga cgatctcacc cacagcacct
      361 gccttctgca gcgccttcaa ctccgccggc gtaatgaagc cgtccacaaa aagaggcgct
      421 tccggcccga gatcgccgat gcccacgaaa gtgacgttcg ccttggccgc cagcgccagt
      481 gtcgatttga tcatcggctg gttgtgcagg agctcccgct cctcgggcga tgacgagatc
      541 accggtaggg gcatcggaaa actgcgcgcc tttacagtgt cggcgagcgt gaagatgacg
      601 ttgtagaagg ccgcggagcc gtccggcgcg atgttgcccg tcagcgagac gatcttgtgc
      661 tgcggacatt ccatcggcgc cagttgctcg atggccgcct tgagggtacg gcccgtcccg
      721 atcgccatga cgatcggctc ggaaggggca agtcggcgct ctatctcggc tgcggcggcc
      781 tgggccacac cgatcgtggt cgaccttgag gccggatcgc tcggcacgac ctccacgtag
      841 tcgagcgcga accgcccctg caatcgccgc ccgagctcca ggcaaccagc gatgggatgg
      901 tctatccgaa ccttgaccag accttccgag acggcgagcg agaccaagcg ctgcgccgac
      961 tggcgagaaa cgccgagctt ggccgcgatc tgatcctgcg tgttgccggc cacatagtag
     1021 agccagccag cccgggcggc atcgtcgagc cggctaccgc cttgatcgtg cctgccacct
     1081 gacgccattc acctcttctc cccgcttgcc acgaagggaa ggtggcagcc ctcgcacgaa
     1141 ccgtcaatat tttcaagcaa acgttcagat tgtgagtatt tgctcgaaca ttccagaaca
     1201 ctcgcctgac aaagagcgcc ctgcaccatc ccggtcgagc tgcaaagtgg gcgcttgacg
     1261 cgattcatcc gcctccgtaa accttgattc gatcgctggg gggctgattc gcacgtcttg
     1321 aagaacggcg cggcgtgtgt tcgtcacgaa ccttcaaggc ttcccacccc tgcagcgtca
     1381 gttctgcgtt tccttgagtt agacgttcgt cagcctgatt tggggtattg ccgccccgtt
     1441 aggcgttcca ttcggattca ccctttcgca cctggaaagg ggaatggttc gggaggttga
     1501 acagcagcga tacgatgacc gtcgccgcac tggagagtcg cactctagca tcgcagtctg
     1561 caattgccgg ccgtcgcgac ctgtcagcct ttatgcggca gctctgtgcc aggaccggcg
     1621 ccgacgccta cgtgcttctc gatgcaacgc ccggcgtagg cccttacgac ccgcccatct
     1681 tggcatccaa ctggacctac gacgccattc agagcacggg tgtcgacacg atcaggcaca
     1741 tcggggaaag ctccgtgtcg gtctatgtcg gcacggagcc gcatggatgg cacccgctgg
     1801 ccctcgccgt ccttttgggg agggaggaga tcgcaaatct tgtcgaattc ggacacgagg
     1861 agattgtcgc cctcaggctg cacatcgcgg gtaagcgcta tcacctcctg ttgtcttccg
     1921 cccgcacggg tgctctcgac tgcacggcga tcttcatcga gcatatgagg tgcggctacg
     1981 cgcttgcgca gttcgcgggc ttgatcagcc ctgacggtga cagcccgctt tcggagcgtg
     2041 agcgcgagtg cttgtcgtgg gtctcgcagg gcaagaccac agaggagatc gctgtcatcc
     2101 tcgatgtttc gtccaacacg atcaacagct atatcgccca cgccattcac aagctggctg
     2161 ccagcaaccg cgccatggcc gtggccagcg ccatccgtcg aaacctgatt tgagccatga
     2221 cagcgaagtc caatacggac gacgaagaaa cgacgatgac ggccgttccc cgatgcggtg
     2281 acgtacagaa gaacctgaag acgcttcccg acattgcgtg cattctgcgc ggcatgaccg
     2341 cggaaattgg cgcggaggaa tttgtgctgt gccttgccgg ccggcgggaa gccgcctccc
     2401 cgctcatccg cgtcatggat tcggcgtatc ctggcatctc gccgtcggcc aaccgcctgg
     2461 tcgcgcacct gcccgggtgg ctcctcgtga aggcgagcgc ctcgctgatc cccctgtggt
     2521 ggaaaggatg cgatgcttcc gcggtggtag gccttgaagc cgcaccttgg gccgagcgcg
     2581 tggaactggc ctcggtggtg ccgggcgtga tcctgccgct ttgcacggaa cgcggccaaa
     2641 agggtcttct gttattttca ggcggtgcaa tttccgttga tcccgatcgg ctgacggcga
     2701 ttcaggcgcg ctgcttcggt ctattcgccg tcgccgtgga cgtttggcca cgccgaaacc
     2761 gcgctggccc gcccatctcc aagcgtgagc tggagtgtct caagctgacg gcaaatggcc
     2821 atactagtga ggagatcggc cgccttttgg gcttgtccgt ccacacgacc aatcagtacc
     2881 tgaccagcac gacgcagaag ctgaatgcgg tcaaccgcgt tcatgcggtg gccaaggcaa
     2941 tccgcgaagg cctgatcaac tgaggaaaac gactacgcgc cggtgcagga atccacagcc
     3001 ttgcggatac gcgcctcgtt gagcgctttt gccaaaaagg cggagtttgc ctccggatcg
     3061 ggcaaagcct cgtcgaagga gacgtagttg acctccacgg tcgcgtgctc ggccataaga
     3121 gtgagcgtga agtagacgga gacgccaggc ggtctgagtt ccaggtcgag tacgatgcgc
     3181 ggctctccgg tccagccgac atgggcctcg cagccgtgtc cgagccgcag agtacccggc
     3241 atgaaataga gctcggctgc cgactccacg atgtcggcga tgctcgccat gctttcgagc
     3301 ctgatgaagg cgacatagtc agccacgtcg atcatgcgca gctcgttcgc cacctcccga
     3361 atggcctcgg cgataatgat ttcgcgtgcg gctgagtgcg gctgccggtt catgcgctgc
     3421 ttctcatcgg ttgccgttgc gccccggccc gatagacgag gcgaatcaac tcggccaccg
     3481 ccctgtagaa ctgaggcggg atcatgctat ccaccgaaac ttgtttgtac atggagcggg
     3541 caagcgccgg ctcctcgaac acgggcacgc catgcgtctg ggcgatctcc cgtatccgaa
     3601 gcgctataag gtcctgcccc ttggcgacga cgacaggcgc gctgtccctc tcccggatgt
     3661 agcgcaatgc gatggcgaag tgcgtcggat tggcaataac caatgtcgct tgcggcacgg
     3721 ccgccatcat gcggtggcgc gcgcggtcac gcgccagcga gcgcagcctt gccttcacga
     3781 tcgggtcgcc atcagtctgc ttgagctcgt ccttgagctc ctggcgcgtc atgcgcaggt
     3841 cttggcgcca gtgaaagcgc gaccacagca ggtccgctga agcgatgacg gccatgatga
     3901 gcgtcaccgt catgagcatc ttgatggtga tgtcgcggat tacgatgccg ccgaactcgg
     3961 caggcggagt gaacatgccg gcaagcagac tgctctgagc ctccctcaag gcaagggcca
     4021 gaaagctgcc ggccaccagc agcttgccga tcgatttcag gaactcgacc atgcccttcg
     4081 cgccgaagat acgcctccag ccctgcctaa gtgagatgtt cgaggccttc ggccggaccc
     4141 gctcgaccac aaagcgcggc gtgttctgca ggatcgaggc ggcgattccg gcgaccacca
     4201 gaagcaacat gacggtgccg agcaccttcg cgatctccaa aaatacgatg cggtagagcg
     4261 cgatcgcgtc atgctgcgtc tcaagcaccc actcctcggg ccgttccatg aagatcgaaa
     4321 ggaaggatgc cagacgcatc gcgctgtcac gtgcaaagaa gatgacgaag accagaattc
     4381 caagaaacga agcgaaaaca ggaagctcct tcgagaaagg taattggccc ttctcgatcg
     4441 tgtcacggat ctttttctcc gtggcttcct ctgtcttgct ttccttgtcc tgctcttcag
     4501 ccattgagca gacagcttac gctgcctctt cctgcttctg cgccgaaggc aggacaaaga
     4561 cgccacgccg ggcgagctgg atggccgccg aggcaattcg cttgcgtgcc cgcgtgacct
     4621 cctcagcctg aaggccgtcc acgtttatcg acaattcgga ttcgatcatg cgacgcgaac
     4681 gaggccccag cgcggacagc actgcctctt tcagctcgct tgccgcgcca cgcaacgcca
     4741 gcgtcaccac atccgtgtcg aaaccgtcaa agagggccac gcgcgccttg tgcggcaaga
     4801 gcgggatctc ctcgaatgag aatatttgtg cacgtatcga ggcgacgtcg cttgtgcccg
     4861 attcctccaa gtcgcgcatg acttcgtcta tctgcgtttt gtcgagttcg ttgagcaggc
     4921 ttgccacctt cgcctgtccg gccgatacgt ccttgccgct cgcctccgcc tgaagcaggt
     4981 tgcgcagttg ccgctcgacg atctgcttcg ccttcggctt tatctgactg gtggaaatca
     5041 ggcgccgcag cacctcggtg cggatcggct tttcgagcgt aacgagaatg cgagccgcgg
     5101 gctcaggact gaggttcgaa agcacgtagg cgatggtctg ggggtgctcg ttcgacaaga
     5161 ggtccgccaa acgctgcgga tcgagcctct ccatatcgcc ccagaccgtc gactgcgaat
     5221 ccacccgcgg tacgttctgc cccatgagca ggccaagttc ctccggcgaa agggactcgt
     5281 tcaagagcat attcatcttg tccgccgaat cgagcagccc ggcaccctcc gcaaattctg
     5341 cctcgaactc gcccacgatt cggttgagtt cggcctgtgg aatcgttcgc agctcccgcg
     5401 cggcctcgac cagcgtcttc aattcctcct gtttgaagaa tttgaggagc cgccctgccg
     5461 ccggctttcc catggcgacc aggatggcag cggccttctg cgcccgcgtc aggttgagct
     5521 tgcctgtcat tcgacgccct gctgtatgca acgagaagag atcatgatca ggctcaatcc
     5581 ggcttttcgt gctcggtgcc ggccacttca gtgagccgga tgccgaagcg cgaagggtcg
     5641 tgctcgagca cggttatctc gcctcgcgcg acgcgtcggc cgttgacaac cacctcgacc
     5701 ggctcgccaa tgcgtctgtc gagagagacg gtcgagccct tctgcagcgc catgagctcg
     5761 gaaaccgtca tttccgccct gccgaggacg atctgtactt ccactgggat acccatgatg
     5821 acggccgcgt tgccaccgaa gcgtccgtcc gcctgcgccc ctccctcctt gccgtcgtgg
     5881 aggacaccgc gcagttcctc gatcgccttg ttgagttgtt cttcttcgag gtcttcggta
     5941 gcgggattgg gctcgttcat tgctgtctcc attggcgctc cgggctgcat ccgtccggcc
     6001 tatcaacccg cggcaagccc ggcgatgaat tcctggccag cgtcgaagcg atccttgata
     6061 cgcactgtgt agttctgccc gagcttgccg aactcgcata tgaaaaccgg ccgcccgcga
     6121 accgaaagtc gcgtttcgga gcgcgcgttc acaggaagct cgatcagttg accgaccctg
     6181 aatgccgcca cgtctccgag agtcatcggc ggcatgggca ctttggcctc gaacgccacc
     6241 gccgagcgca tcagctcctc gttgaagcgc tgtctccact gtgcctggtt tgctgtctcg
     6301 tcggtactgt ttcccgtcgg cgatcgctcc tggaggagga cacgctgcgg catgagggcg
     6361 cgcacctccc catcgccaca tgccgaagcg acggaaaaga ccattcgcac gccgggtcca
     6421 tcgcgaacgg caagccgctt caagccttct cctgcaaccg gcgcggcaag tggaaagcgg
     6481 agaccgaggg cgcgagcccc gtggccgttg atggcttgcg cgaaaatctc cagcacctgg
     6541 tgcgtgacgt ccaattcgat tgccgagagc tcacgctcga tcggaatcgg cggatggtta
     6601 ggatcgccac cgagataggc atcgagaaag agcgatatcg cgcggctgtc gatctgcaga
     6661 gtgagtgcgt caggcgacgt agcggagccg gccaccgcga cggcctcgcc gctatcgggt
     6721 gcaggtatgg catcggcgag gcggattagc tccaccgact tcaattcgac gtcgagctcg
     6781 aacgaaagct gctccgacag tttttccagg acgaggggca ctgcgcgagc cgcgctcttt
     6841 cgcgcagcgg caatcaccct gtcgggatcg tcggtggcgc ctatgagtcg ctcgacgacg
     6901 aggcggcgca taatctccgg gtcgtcggac tcactcatcc cctaaccctt gttccgttcg
     6961 atcggccgca tgtctcatgc cgccctgctt tgtgcctcgt ccccaccggg attgagggtg
     7021 ctctgctcga cctcatcgat cgtcggtcgg tccttcgacg agatcgtttt tcgtccgaac
     7081 tccagcgcca cctgcggcag cgagccgttc atgtacgcaa gaagggtctg cttaacgatg
     7141 acataaagcc ggttcttctt ctcgcgcacc accttcacct ttgccgcaat cggcccgaca
     7201 acagcgtagg acataaagat accgaggaag gtgccgacaa gcgcggcgcc gatcagcgcg
     7261 cccaagattt ccggggactg gtcgagcgct cccatcgcct tgatgacgcc gagcaccgcc
     7321 gcgacaatgc cgagcgccgg caaaccttcc gcaaccgaga tcatggcgtg ataggatttc
     7381 agcttgtcgc gccggatggt ctggatttcc tcgtccatca gagcctcgat ctcaaaggat
     7441 cgggcgttgc cgatgatgat gatgcgacaa tagtcacaga tgaaatgcgt cagatcgcgg
     7501 ttgttcagga ccgtcgggaa tctctggaag atcgctgatt cctcgggatt atcgatatgc
     7561 gcctcgacct ccgtgcgcgt cttcgtgcgc agctcgcgca ttaggctgtg cagaacgccg
     7621 agtgtttcga gatattcctt ttcggtgggt acgtcgtgtt tgaaggcttc catgcaggcc
     7681 ctggcggcat ccttcaccgt cgacatcggg ttggcgacca ggaacgtgcc gaaggctgcg
     7741 ccgcagatga ccactgcctc ccacggctgc aaaagcacat ggatatgccc gcccatggcc
     7801 ataaagccgc ccagcacgca gcccacggtt acgacaagcc cgataatgat acccacggca
     7861 ccctcgcaga agatcgatct cccaagggct acagaccctg ccttgtgcga ggcttgatac
     7921 cgtgccgagc ctctcccgca tcagcctcgc gcaaggcagc gtgtctaccg ttgccgcaag
     7981 caaacctgcg cgaggagccg cgttttgttg accaccttcg ccagttacca gctcatagtg
     8041 cgcgacataa acaagtcgat cagccgcgtg gaaaaccaac cgctcgtcag gcgcgagacc
     8101 gagtactacc tggcaaatat cggcaaggta aaatcgatcg aggagttcgt ctccgacgac
     8161 cgcctcttcc gctatgccat gaaagctcac ggcctggagg atatggccta tgccaaagcc
     8221 ttcatggtca aggcgctcaa ggaaggcgtg agcgatcccg acagctttgc caaccgactc
     8281 acggacaagc gttatgcgga attcgtgaaa gcctacaact tcgccgcgct gggtgaggat
     8341 gcaaccattt tcaatccggc gcagcaggga acggcggatc gctatcttct cgcagcggtc
     8401 caggccggag tcgatcctga cgacgagctg ttgaaccagc agatcgacta ttacatggag
     8461 aacatcgtca aggtgcgctc catcgacgag ttcctggccg acgatgcact ctacagcttt
     8521 gctctgaagg ctttcggcct ggctgaccgt ctcggcgaca aggacttcat cagggagttg
     8581 ctcgaaggcg gtgtcgacga tcccgacagc ctggccaatc ggcaaaagga caaacgctac
     8641 gcagaattcg tggccgcctt caactttgcc cgttacggcc cggatgcgac aacgatcaat
     8701 ccggcgttgc agccgacggt cgacgcctat ctgcgccaga cgctggaaga gaacgcgggc
     8761 gagcagaacg agggcgtgcg ccttgccctc tatttcgaga gaaaagcccc agacatcgat
     8821 tccttctatg acgttcttgc cgacagggcc ctcgcgcagg tggtgcgcac cgcactgggg
     8881 ttgcccgagg cgatcgcccg gacagacatc gaccgacagg tggcgatgat ggaagcgcgt
     8941 atggatatcg gcgattttca ggaccccgaa aagctcgatg acttcctcaa acgcttcacg
     9001 agcctgtggg agataacgaa tccgtccacc agccgagagt cctccgtcag cgtactgttc
     9061 ggtcaaccgg ccgaattcgg gatctcgaca gacctcctcc tgaccatggc gcaattgaaa
     9121 cgttaaggcc cagccgtgca gaacagcctc tacgtagccc tttcctccca gatcgctctt
     9181 cagcgtcgac tggacaccat cgccgacaac gtcgcgaatg ccgggacggt cggcttcaag
     9241 gcgaccggca tagagttttc tgacgtcgtt gcaggtttcg gcgatgatgc ggtttcgttc
     9301 acgtcgaccg gtggaaccta tatcggctcc cagcccggta cccttcgcga gaccggcaat
     9361 ccgttcgatt tcgctgtgca gggggatgcc tggttcgcga tccagacgcc ggcaggcgtg
     9421 gtgatgaccc gtgacggccg tttcacgatg ctggatacgg gcgagctagt cacactgaac
     9481 ggctatccag ttctggatcc aggcggcacg ccgatcgtgc tcaatccgcg cggcgggcca
     9541 cccgaggccg cctccgacgg cacgctgcgc cagaacggcg aactgctcgg cgcgatcggt
     9601 ctctacgagt tcaatcccgg cgagaatttc atccgtttcg agaactccgg catcattccg
     9661 caaggcgagc cgcagccgat cgttgatcgc cccgagatag gcgttgcaca gggattcatc
     9721 gagcaatcca atgttgaccc ggtcaaggaa atgaccaacc tgatccaggt tcagcgcacc
     9781 ttcgagcagg tgacagcact gatgcgggac agcgaaaccg cgcttgagga tgccatcaag
     9841 acgctcggct catagccgcg aggagaagag ctgggcggat ggaaaccgct gagaccaaca
     9901 tcgggcgtga gggcgccttg ggcgccgcga atgcactttc cgctctggaa gaggcgtatc
     9961 gtcgcttcat tgatccgcgc tttattctca agcgtggcgg ccgtatcgcg gaaatctcgc
    10021 caacccacta tcgcatccgc ggcctgtccg actcggcacg acttggcgat atcgtggaat
    10081 acgggccacg ttccaggccg cgcacggccg aagtcgtgca catcgcacga gaagaagttc
    10141 tcgttgcccc ctttgagaac gatggtacgg cgggcctcga cgaagctgtc ttcaaccggg
    10201 gacctgtcca catcacgccc catgcgagct ggcgtgggcg tgtaatcgac gcactcgggc
    10261 ggccgattga cgaccggtcc cgctcgctca actatgagcc cgagcattcg gacgatcggc
    10321 ggaccccgcc cgctctacgc cgccagcgcg tcgccgagcg ctttctcacg ggtgtccgtg
    10381 tgatcgacat cttcacgccg ctttgccacg ggcagcggct cggcatcttc gcgggttccg
    10441 gcgtcggcaa gtctacactg ctggcgatgc tcgcccgagc tgaggcgttc gacaccgtgg
    10501 tagtcgccct gattggcgag cgcggacggg aggtgcgcga gtttctggaa gacacgctgg
    10561 ggcccgatgg catggccaag acggtcgcgg tggttgccac cagtgacgag agtcccctga
    10621 tgcgtcggcg cgcggccgac acggccatgc gcgtggccga atcgtttcgc gaacgcggcg
    10681 aaagagtact cctcatcctc gactcgatca cccgcttcgc ccatgccctg cgcgaggtgg
    10741 ccatcggtgt gggtgaaccg cctgtggctc gcgggtatcc ggcctcggtc ttcaccgcgc
    10801 tgcccaaact tctcgaaagg gccggccccg gcgaaaccgg cagcggatcg atcacagcga
    10861 tcctgtcagt ccttgtcgat ggcgacgacc acaacgatcc ggttgccgac tgcgtgcgcg
    10921 gcattctcga cgggcatgtc gtgctcgaca gggggatcgc ggagcagggg cgctacccgc
    10981 ccgtgaaccc gctggcttcc atctcacggc tcgcagaccg ggcctggacc ggagagcaga
    11041 aggttctggt ccgccagctg cgtgccatga tctcgcggtt cgaggacacg cgcgacatcc
    11101 ggctgcttgg tgcctacaag cccggagcgg acgccgagct cgatcaggca gtgcgtcagg
    11161 tacccgcaat ctacgaagcg ctcagacaga gccccggcga acggccctcg ctcgacccgt
    11221 tcgccgatct ggcgcgcgcg ctcagggcaa tggagaacaa tgatgccgga cacgtcggct
    11281 gagcccgaaa gcagcaagaa ggttcatgtg gaggagcatc cgaaccttcc cggcatgcag
    11341 ggcgaccttg cgggctcccc gccgtcccgc ccgcggatag cagccagttt cgcatggttt
    11401 ggtcgcacgc tcggcagagg gcttacaagt gacgtcggct tggccacggc aggcgtaatg
    11461 ctggcggcaa tctgcgccct cttcccctgg tatatcttcc tgcaccagga ccagttcggc
    11521 atcaggccac tcgaattcga gggtcgcgca atacagtcga gcccatcacc cgccgggcag
    11581 ccaaggccca taggcacgca tgtcgcactt gaggagatcg aggccctcgg cctcgatgcg
    11641 atacccacag gcactgtcca tcgcgatgcg gagcctgtat cggctcctgc tgccgaacag
    11701 ccctatcccg gcgaggaagc cgtcttcgag ctcgtctacg tcgccgccgg ccgcgccatg
    11761 atcagggacg gttccggctt ctgggtcgtc cagcccggtt cgcggctgcc cgacggaagc
    11821 cgcctcgtct ccatcgaaaa gcgtgacggc aattgggtgc tcgtcacctc gcggaaaacc
    11881 gtgatctccc tcacgcggta gggtcccccg caccccgatg cgagcccgcg ccctggacgc
    11941 aaggcccgcg caagactggc agcctacgct ctccccgtcg cgaggagagt cgaatgcagc
    12001 ccgtcaacct gttcgatctg gccgcacagc aggcgcgatg gctttcggtc cgccaggccg
    12061 cggtggccgg caatatcgcg aatgtgaaca cgcccggata ccgcactgtc gtggtcgaac
    12121 ctttcgacga ggttctggac aacaggcgcg tgtcgctgcg tgcgactcat ccggatcacc
    12181 ttacgacggg tgccaccggc aatgcctttg acctccgtcg ggccgagccg gacacggccc
    12241 ttctgccgtc ggaaaacagc gtagtgctcg aggaggaact ggtgaagcag agtgagatcc
    12301 gccgtgcctt cgagctcaac acggcgatcg taaaagcctt ccacagcatg ctgatgatgg
    12361 tgacgaggag ctgacagcga tggatcctct cgtaggcgca ctcaaagtcg ccgcctcggg
    12421 gctgggcgcg cagtccgaac gcatgcgagt cgtgtccgag aacatggcca acgcctactc
    12481 gacgagcaat gtgccggggg gtgacccgta tcggcgcaag acgataacct tcgccgccca
    12541 tctcgacgag gccaccgggg gcagcctggt cgaggttgag tcgatcgggc gcgatccttc
    12601 ggcttttcca gtcaaatacg atccaggtgc cgaggcagcc gacgaattcg gctatgtcaa
    12661 gatgccgaat gtcaacatct tgattgagat ggccgacatg cgcgaggcca accgcgggta
    12721 cgaggccaat ctccaggtca tcaagcaggc acgcgaactc gtgtctatga ccattggcct
    12781 gctaaggagt cagcaatgat ttcggcgctc ggctccataa gcccggtcac ggtctccggc
    12841 ctcggaaacg accgaccggc gacggtcagc gtggctcctc ccggcggtgg cgaagaagac
    12901 ttcggtagcg tgcttggcga aacgctggga cgaaccgtcg ctcagctgga gaacgctgaa
    12961 aagctgtcga tccaggcgct acggggcgac gccgagatca gggaggttgt cgacgcggtg
    13021 atggcagccg agcaaacgct ccaggcagcg atcgccatcc gcgacaagat cgtgaccgcc
    13081 tatctcgaaa tcagccgcat gtcgatctag tgcagacgga cttggagttt gaccgatgaa
    13141 ggctctcgcc attgccgcga cgggaatgag cgcccaacag ctcaacctcg aggtcatcgc
    13201 aaacaatatt gccaacatca acaccaccgg cttcaagcgg gcacgcgcgg aattctccga
    13261 tcttctgtac caggtcgagc ggcttcaagg cgtgccgaca agggcggacc agggcatcat
    13321 ccccgagggg gcaagcgtcg gacttggcgt aaaaacggtg gccgtgcgca acctgcacca
    13381 acagggctcg ctcacgaaca ccggaaacaa gttcgatctg gcgctggtcg gcaaaggttg
    13441 gttccagatc gagggcgccg atggcgagcc gctctatacg cgcgccggga ccttcaacac
    13501 cagtgcgacc ggccagctcg tcaccgtcga cggctttccc gtccagcctg caatcgtcgt
    13561 gccggaggac gccgttgacg ttatcatcaa caaatcgggc caggtctttg cgcgcattga
    13621 cggccagacc gaccttcagg agctgggcca gctgacgctg gcgaactttt ccaacgaggc
    13681 aggtctcgag ccgatcggca acaatctctt ccgtgagacg gtggcatctg gtccagcgaa
    13741 tgtcggtgtt cctggagatc ccggctttgc cgaggtcgaa cagggctatc ttgaggattc
    13801 caatgtcgat ccggttaagg aaataacaga gctgatctcc gcccagcgcg cctacgagat
    13861 gaattcgaag gtgatcacgg ccgccgacga gatggccgcg acaattaccc agtccatgcg
    13921 gtagcagaca agatgcgacg cgtaactgcg ccttgcctcc ttctgttcgt cctccttgcc
    13981 acggccggtg cacccgcttc cgcccaagag atggcggtgg tttcgacacg cgttatctat
    14041 cctggccaga ccattcaggc cgacgcactc aaagatatcg tactgaagcg accgccgcgc
    14101 gggatatccg ccatcgtgcg ttcaagcgac gaaatcgtcg gcaaggtggc gcggcgcacg
    14161 ctgcttcccg gccgactgat cccggaggga tcggtccgtg agccgtatct cgtcgaaacc
    14221 ggctcaccgg tcaccgtgat cttccgcagt ggcgctcttt cgatctcgct catcgccgtt
    14281 ccgctcgaat cgggcgccgc gggcgacgtg gtcaagctgc gcaacatgga tagcggggcg
    14341 gtcttttcgg gggtcgtgct ctccgacggc acggtaaggg tcggcaccac atgatcctgc
    14401 gcgcgctgat cggttccttc ctcgcattgg cagcggcttt cccggctgcc gcttcatcgc
    14461 gcatcaagga catcgccacg ctcgaagccg cacgcgacaa ccagctcgtg ggctatggtc
    14521 tggtgatcgg tctgcagggc agcggcgaca gcctgcgcaa ctcgccgttt accgagcagt
    14581 cgatgcgcgc catgctgcag aacctcggaa tcgcgaccga gggcggccgt atgcgcgcca
    14641 aaaacgtcgc tgcggtcatt gtcaccgcca atctgccgcc tttcgtgcag tcgggcgccc
    14701 gcatcgacgt gaacgtttcc tcgctcggcg acgcgacatc cctcgccggc ggaacgctcg
    14761 tcatgacgcc tctcagggcg gccgacggtg aaatctacgc cgtagcacaa ggccccgtca
    14821 ttgtctcggg cttcaatgcg gaaggcgaag cccagaagct gacacagggt gtaccgacgg
    14881 cgggaagggt gccgaacggc gcgatcatcg agcgcaaggt ggaggcggcc ttctcgcata
    14941 tcgacaccct caccctgcag cttcgcaatc cggatttctc gacggctgtg cgcgttgccg
    15001 atgcgatcaa ctcctatgcg acgaggcgct tcggcaagaa gcttgcggtc gagcgggacg
    15061 cgcgcacggt cgtcgtccac aagccggcca acatttcgac ggcacgcttc tacgctgagc
    15121 ttgaaaacct ggtcgtcaag ccggatacgc ctgcccgtgt cgtcgtcgac gaacgcaccg
    15181 gaacggtcgt catcggccag gatgtccgca tttcgcgcgt cgccatcagc tacggcaccc
    15241 tgaccgtgcg gattacggaa atgccccgcg tggttcagcc aaacccgttt tcgcgcggcg
    15301 aaaccgcggt cgagcccttt acctcgatcg aggcggacca accggatgcc agggtggcgt
    15361 tgctcgacgg cccaaacctc gaaaccctcg tctccggtct caaccggttg ggtgtcaagc
    15421 ccgacggcat catcgccatt ctgcagggga tcaagtcggc cggcgccctg caggccgagt
    15481 tggtgctgca ataggtgcgt cctatgagca tcgtcgcacg tagtcgacat tgtttttgcc
    15541 gcggagcaaa tgccgcagcg ccgctggtgt tcgcgctcat gcttgcgcag gccatacccc
    15601 cgcccgcgct ggcggccgaa gccgggagca gccagccggg cggcgagatc cagcgcttct
    15661 gctccaacat cgctgatgcc gcgcgcgacc gccgctacgc gctgcagacg aagcggctgg
    15721 aaaatttgaa ggccgaaatc gacaagcgcg tcgaagcact cgagaaaaag cgcgccgaat
    15781 acgaggactg gctgagacgc cgcgaagaat ttctggccaa ggcagaggat aatgtggtgg
    15841 cgatctactc gaacatgaca cccgacgccg ccgccgagaa gctggcagcc ctcaatgtgg
    15901 agctcgctgc cgcgatcgtc atgaagctcg acccgcgaaa ggcgggcgtg atcctcaacg
    15961 agatgaacag caaggtcgcc gccgcgctga ccggcgtcat ggcggatgcg gctcgcagga
    16021 acgatccctc atgatgcgcc ttcttccggt tgccgcctcc tgtattgccc tgcaggcctg
    16081 tggtgccaat ttccaggaga tcggcgttcc gccggcactg tcgcccgtcg gcagcggcat
    16141 tcatcccgca gcactgtccg acatgcagta tcctcaagcg ccgtcccggc cggtcacgcg
    16201 gttttcgctg tgggacgaca agcagagcca attctacacc gacgcacggg ctatcaatat
    16261 cggcgacatc ctcaccgtcg agatcagaat gaacgatcgg gcggagttcg agaacgaatc
    16321 gaagcgcaag cgcgattcag ttcgcaccct gggcctcgcc ggttcctatt ctgcaggcag
    16381 ttccggctcg tctgcctcgc tcgacggcca aatcgattcg gtaacctcct cggccggcag
    16441 cggcagcacg gagcgttcgg aggagatcaa gctgcgtgtc gcggccgtgg tgacgggtgt
    16501 gctgcccaac ggaaacctgc gcatcagcgg ctcccaggaa gtgcgcgtga acgcagagct
    16561 tcgtgtgctt acgatcgaag gcatagtgcg gccaagcgac attggctccg acaacaccgt
    16621 gtcctacgac cgcatcgccg aggcccgcat ttcctatggt ggccgcgggc gcctgaccga
    16681 ggtgcaacaa ccgccttatg ggcagcagat tctcgaccag gccctgccgt tctgacgatc
    16741 cggaggtaca gcatggccat agcagagaaa ggggaagcgc aaaggaccgg accgccgctg
    16801 gtcgtgcaac tggtcctgtt tgctgccctc acgttcatgg ccgcggggtt gggctggctc
    16861 accggcagca acctgatccc cctagcggct gagaagagca agccgggaac gataacgagc
    16921 gatcgtgagg agaccacctc ccctgcagac gagccggccg aaggggaggc tgtcgagccc
    16981 aatctctaca cgctggaacc aattaccacc aaccttgccg atccgcgcga catatgggtg
    17041 cggctggaag tctctctcgc cttcgaaggg ccggcagatg caggcctggc ccaggagatc
    17101 caccaggaca tcctggccta tctgcgcacg gtgaagctcg agcagatcga caccgcgagc
    17161 ggcttccatc atctcaaaag cgatctcgag gaacgcgccg ccatccgcag taacgggcgc
    17221 gtcaccgaca tcttcatcag gacgctgctg ttcgaatgaa acgtctttgc ccgacagcgg
    17281 cgatcctgct gatgagcggc gcaaccgcaa gtgcccagac gttcgatctc ggccagctcg
    17341 ccagccaggc cgacggcacc accgtcggct acatcataca gcttttcggc ctgcttacgg
    17401 tgctgtccat cgcgccgggc attctcatca tggtgacgag cttcacgcgc ttcgtcatcg
    17461 ccttttccat tctgcgctcc ggcatcgggc tgccgacgac tccggcgaac ctgatcctca
    17521 tcagcctggc gcttttcatg accttctacg tgatggcgcc ggctttcgac caggcgtgga
    17581 acgacggcgt gcgcccgctg atgaacaacg agatcaccga ggagcaggcg gtcaagcgca
    17641 tatccgatcc tttccgcacc ttcatgatga acaatgtgcg cgagaaggat tacgcgctgt
    17701 ttgccgacct cgcgcgcgca cgcggcgagg agaacgtcgc tgtcgagcag ggcgacctca
    17761 ggatcctcat tcctgccttc atgatctccg agctacggcg cggtttcgag atcggctttt
    17821 tgatcgtact gcccttcctc gtcatcgacc tgatcgtggc gacgatcacc atgtccatgg
    17881 gcatgatgat gctaccgccg acggtggtct cgttgccctt caagattctc ttcttcgtgc
    17941 tcatagacgg ctggaacctg ctggtgggca gcctggtgcg ctcattcacg taaaaggtcg
    18001 tctgggccgg caaggtcgcg catgccgccc ctcgtcctgt tcagaagcac gagcgtccgc
    18061 aatcgcctcc tcctgtcggt ggccagcgat tgccttagtg caggtgcctc agcttctcag
    18121 gattgcgcac gacgtagatc acagcgatct tgtcgtcctc gatctcgagc gcggtcgtct
    18181 ggatctcgcc gtctgcctcc agcgtgatga accccggcag accgttcacg aaacctgttc
    18241 ggatgagctt cgagccattt tcctggaaca gagccgccag acgttcctgg atcttcatta
    18301 ccgcatcaaa cccgacgatc ggcttcatag ccgccgggcg cttgccgcca ccatcggcgt
    18361 gaatgctgac atcggctgca agcatcgccc cgagcgcttt catgtcgccg ctgcgtgagg
    18421 cgacgaagaa ggccttcgca agctcgagac cacgttgctt ttccacctga aaacgaggtc
    18481 tcgcctcccg gacatgggcg cgcgcccggg cggcgagctg gcggcaggct gccgggcctc
    18541 gctggatggt cgctgcgacc tcctcgaacc ccagtccgaa cacatcgtgc agcaaaaagg
    18601 ccgcccgctc gagcggtgaa aggcgctcca gagcaagcat cagcggcaag gtgacgtctt
    18661 cttcctcttc ttcctccacg acgggatcgg gaagccaggg accgatatag gtttcgcgct
    18721 gacgtcgcgc cgatttgagc tgatcgaggc agagtcgtgt gaccgtgcgg cgcagaaacg
    18781 cctcggggtc gcgcacctcg tcgcgatcgg tcctcatcca gcggatgaag gcctcctgca
    18841 ccatatcctc cgcgtcagcc acagagccga gcatgcggta ggcgacgcgc atcagcttcg
    18901 gacgcagcgg gtcgaaaccc gccgctgcgt cctcgttgat ggcacccgtc atcaggcagc
    18961 cgctttcgca gccgccttta ctgctgccgg atcgacccag ctgccgaaac cgaccgcgag
    19021 gcggttccac ccgttaatga cgttgatcag caatgtaagc ttgacctgct cttcttccgt
    19081 gaagtgagcc ttcagggctt cgtaggcgct ctcgtgctca tgcccttccg acaggcgcgt
    19141 cagggcttcg gtccaggcga gcgcagcgcg ttcacgatcg gtatagcaag gcgcctcgcg
    19201 ccaggccgac agcagataga tacgctgttc ggtttcgcca ttctcgcgcg caaaaaccgt
    19261 atgcatgttg agacagttgg cgcaaccatt gatctgggag gctcgaattt tcaccagctc
    19321 gctgaggctc ggctcgagac tggagtcaac ggcgagcgac agacgtcgcc attcggtcat
    19381 cagtgaaggg gcagcggcaa agggatcgag tttggcagtc attatcggct ccttctgttg
    19441 gttacgatac catgacgaga caaaatccgc tggtgtgaca cgcacgggcc agaaaaggct
    19501 cctagcatga gtggccggtg accagcactg ttggctctcg taccgatcac acgactgtga
    19561 accggggcat ggttggtttg cgtcccgtac catgcaccat gccgctctcg cgaatcgtga
    19621 agcgcgggct cgccgatatt cggccttcgg tccaaaatca ctgatcacga ggagacgatc
    19681 taatggcttt cgacagaatt tgggcgttgg cgccgcaatg gttgagcatc ttgaggatca
    19741 tgtcggcgct gctgtttctg gaacacggaa cccagaagct cttcaatttt ccgcccgctc
    19801 ccgaaccgat ggaactatcg gggctctttc tgctccaggg actgatcgag gtggtcggtg
    19861 gcatattgct ggcaatcgga cttttcaccc ggccggtcgc gttcatcctg tccggcaaca
    19921 tggcggtggc ctatttcatg gcgcatgcac cgatgaattt cttcccgatg ttcaaccagg
    19981 gcgatgcggc tatcctctac tgctttgtct tcctctactt cttcttcgcg ggaggtggtg
    20041 cgtggagcgt cgatgccgta cggggcggaa gcggcctcgc caagccttga acggccttgg
    20101 gcagccgtcg ggaaaaggca ggatgtacct ccggcccgaa cgccacgccc ggtacaagcc
    20161 caggaggcgg accctagccc tggggatttc cccggctgtc atcggtgcgg ttgatcaccg
    20221 cccagatggc cacggccggc gtgtcgccga tcgcccagag ccggtgcggc aacctggcat
    20281 tgaacgagac ggagtcaccg ggccccagtt cgtaggtctc gaagcccacc tgaaggccca
    20341 gcctgcccga aatgatatac gcatattcct tgccgccgtg ccggaagagg gaatcggctg
    20401 ggcaggactc ggcccccacc tcgtatacga catggagaaa atcgaccgtc tcgtcaggct
    20461 gcgccgtcaa tcgttcccac acgaccccgc cagccagccg gatgcgcttg cggtcgtggc
    20521 ggcgctgcac tggtccttcc gagccatgtg ccttcgcacc ggtgggaggc ggcggggccg
    20581 tagaacgttc agaagcattg aacagctcgt cgatcgtcaa atcgagctcg gaagcgatcg
    20641 accaaagcgt gctgacggat ggcgtgacga gaccgcgctc gatctgcgag acgagactcg
    20701 gtgacacgcc gatccgtcgc gccagctccc tgaggctcag tccgcgcgct tcgcgttcct
    20761 gcctgaggcg tttaccaagg aaatcgagcg ccggcgcgcc cgtctccggg cggttcgcta
    20821 tgccggcggc ggcaccgctt tcctgtggaa cgtcatcacg gtcttcgttc ttcattgcac
    20881 agcttcagcc ttgttggccg tcctgctctt gacaggccta ccgttgatat acattaaaca
    20941 tagtacatta aaactgaaca cctcaaattg cgctttggga ggagcaagtg gtccattacg
    21001 tcgcgagggc tgccgacatc aagctctttc ccgcctatcc ggggaagagc ctgggttaca
    21061 ggcgcagctc cgtcgtcgac cgttcggtcg ggtcggtgca caccgcgctc gggctttgcg
    21121 agctggacga aggcggccat atcgacgtcc acgtccactc cttcgaggag cagttctaca
    21181 tcaccgaagg caagccgacc ctcgtgctgg atggacgagg ttacgcgctg gatcccggcg
    21241 cctgcggcgt gattccggtc ggtacgccgc atgcctggct ggggccggcg ggagggacgg
    21301 ccaaatggat agacttcatg tcgccgattc cgcgcgagaa cggaccgcga gacaccttct
    21361 ttctcggccc ggccggcgac gtgaaggttg ctccggtcga tatacgcgat ccgcgcacca
    21421 ggcacttctt ccgcatggcc gatgacgata tcgacgtcga gaagctcaag gtcggctcgc
    21481 gggtggatgc tccgaaggtc tctgccagca tggccaccgc acttctggcc tacagcggaa
    21541 ttgccgtgaa aatgctggtg gaccagcggc tttcagccgc tctcggcacg atgttcatgg
    21601 tcgaatacca gcccggaggg gtcgcacatc cccacgatca tccgctcgag gaggcttacg
    21661 tcattcttca cggcgaggtc gaagccgtgg ccgacgacaa gacctacacg ctgaagacgg
    21721 gtgacgtgct gtggaccggc gtcggctgca tccacgcctt ctataacaga accaatacga
    21781 ccgtgcgctg gctggaaacg cagtcgccgc agccgccggc gcgccactcc taccgcttca
    21841 gccgcgactg ggagtacctc gaagagaaac tcgcggagca gcactagcat ggcaaacaat
    21901 gcgatcatca tcggcggaac ccagggcatc ggactggaga tcgcccagca gatggtccaa
    21961 cgcggcgatc aggtgatcat caccggccgc gacaaggccc gcacggaaaa ggtggcggcg
    22021 gaactggggc acggagtcaa ggcgctggcc gtggacctgt ccaagccgca cgacatcgct
    22081 gccgcactgg cggatgtgcc gcagctcagc catgtggtga tcacggcaat cctgcgagac
    22141 gcgaactccg ctcgtaacta cgacgtcgac cgggcgctca acctggtgac gctcaagctg
    22201 gtgggttaca ccgaagccat ccatgccctg ctgcccaagc tgaacaacga tgcttcgatc
    22261 gtgctgttcg gcggcctggc caaggaacgg ccttatcccg gctcgaccac ggtcaccacc
    22321 gtcaacggcg gggtcgccac gatggtgcac acgctggccg tcgagctcgc gcccatccgc
    22381 gtcaacgcga tccatcctgg cattattggc gacagtcctg cctggtcggg caagaacctg
    22441 gatgccgtcg ttgcccgcac gccgaccaag cgtcttgcca ccatgcgcga cgtcgccagt
    22501 gctgcgcttt tcctgctcga caacaattcg atcaacggcg tgaacctcaa tatcgacaac
    22561 ggttggctgc tgacttgagc agcgaacgag ggaggagact gaaatgaaga ttacgagaag
    22621 gcgtttcacc aagctcggcg cagccgcctc cataggcctc gccgcacctg ctgtctggac
    22681 caaggggttc gctcagagcg ggaagatccg cgtcggctgc agcgcctcgc tgaccggtcc
    22741 gctctccagc acgaagaaca tggtcatcgg gtacgagctg tggcgcgatg acgtcaacaa
    22801 ggccggcggc ctgctcggca aggaagtgga gctcatcgtc tatgacgacc agagctcccc
    22861 ctccaacgtg ccggcgatct attccaagct cgtagacgtc gaccaggtgg atttcctgtt
    22921 cacgccctac ggcgcgaacc tcaccgccac gatcatgcct ttcgccaagc agcgcgaccg
    22981 cttcatcgtc ggcatcctga cgctcgccag caacgaccat ctccggcacg acaagttctt
    23041 ccaggccgcg ccctggggtc ccgacggcgc aaccaactgg gcgcgcggct tcttcgacat
    23101 cgcgcagagg gatggtgtca agaagatggc catcctggca gccgacacgg agttctccaa
    23161 gaccgcggcc gaaggcggac gccgcgtttg cgaggaaacg ggtatggagg tcgtcgactt
    23221 ccagatgtac ccgccgacca accgcgattt ctcctccatt ctccgcaaca tcagggcagc
    23281 cgagccggaa gctgtgttcg tgtgctccta tcccgccgat tccaccgcca tcgcccgcgg
    23341 catccaggag atcggcctcg gcgattcggt gcgcctgttc ggtggcggca tggtcggccc
    23401 gcaatacgcc tcgctgctgg aaaacctcgg gccggctctg aacggcatcg tcaacttcca
    23461 tctctatgtg cccgagccga ccctcaagtt cgagggcatc gacggcttcc tcgcgcgtta
    23521 cgtgccgatc gccaaggagc ggcaggtcga tccgctgggc ttctatattc ctccgttcac
    23581 ctacgcggct ggccaattga tctccaaagc cgtcgaggcg accggctcga tcgatcaggc
    23641 ggaggtctcg cagtggctcc acgaaaattc cgtcgatacc gttgtcggtc agatcacgtt
    23701 cgacgagatc ggtaactgga ccgagcgccg cgtgctgatg gtgcaattcc agggcgtgga
    23761 cgacagcggc atcgagcagt tccgccaggc cggcaagcag gtcgtcatcg atccgaccgc
    23821 ttatgccagc ggcgacttga tcccgttcgc cgaagcccgc aaagcatgac cacccttgca
    23881 aacggcaccg tcctggcgga cggtgccgct cgcgcgtttc aggagtcagg atggtcgtcc
    23941 ttgacctcgt actgaatgcc ctggctaccg gcgtgatgct cggcagcatc tatgcgcttc
    24001 tggcactggg cctcgccata actttcggta ttttgcatat tcccaacgtg gcgcatccgg
    24061 cgctcgtaat cgccggcgcc tatgcggtcg tgacctctaa ccggttcggg ctcgatccga
    24121 tactggccgg tgtgttgtgg gccgtaccct tctacctcct cggaatcctg ttctacgaat
    24181 tctactcgcg ctcgttcgaa tcaagaggcg gcggcaatac gctgcaaagc ctgacgctgt
    24241 tcttcggcgc ctcgctcatc gtcgaagtcg ggctcgtagt cgcctacggc accgacttca
    24301 gatcggtgaa tgtcgactac gtcggctcca gcctccgcct gggcatggtc gcaattccat
    24361 accgtttcct gattccggca ctcatggctc cggtggcggt gtttcttctg tggctctatc
    24421 tcagccgcac gaacatcggc cttgccatcc gggcggtcgc gcatgacgaa cgcgccgttt
    24481 ccgtttccgg catcaatccg gccacgatca agcgccatgc cttcggcatt gcgacggcgc
    24541 tggcggcaat cgcgggggcg gcgctggtca taaccggtcc gatcgacccc tttgccgggc
    24601 gcatccatat cgggcgcgtc ttcgccatcg tggttcttgc gggcatggga accataccgg
    24661 ggacgctggc cgcgggcatc ataattggca tcgccgaatc gctcgtcatg agcttcatga
    24721 gcccgtcttg ggcgccgggc gtggcctttg ccatactgct cgccacgctc gccctgaaac
    24781 cgaccggact gttcggagcg gcacgatgac gatggcgcga ttcacaatgt tggcgctgct
    24841 ggtcttcgtg gccgggctct ttctgccgaa gttggtgtcg aatccctact atttctatgc
    24901 cggctacgtc gtgctgcagt atgtggtgat cgccaccggc tggaacatac tcggcggcta
    24961 cgccggctac accaattttg gcgcttccgc ctttttcggt gcaggcgcct ataccgccgc
    25021 gtttctcttt aaagcgctcg gcgtgtcgat tttcgtccag atcctgggcg cgtgcgcagt
    25081 cggcatgctg cttggcctcg cgaccggcta tctgaccctg cggattcagg gcgtctattt
    25141 cgcgatcgcc accgtggcgc tggtgatcgt tctcgaaacg ctggtccaca acacggaata
    25201 tctcggaggg gcatccggca tgacgcatct ggcgccccct ccgccggcct ggttcggcgg
    25261 cacgacccag tacgtgttct gcatcatgct cggactggcc gtggccgcgg tggtgctggc
    25321 gcgctggctt gaagtgtcgt gggtcggccg tggattccgc gccattcgcg caagcgaacc
    25381 ggcggcagag gcttgcggcg tgccgacatt gaagctgaag cttctggctt gcgcgctcag
    25441 cggcggcgtg atggcagccg ccggcgcacc ctatccgacc tacacctttt atctcgatcc
    25501 tgtcagcgcc ttcagccttc tgatcgggct caacgccctg gcaatgccct tgatcggcgg
    25561 tacgcgaaca tggctcggcc cggttatcgg agcggttctg ctcgccagcg ttcagcaact
    25621 ggcgacggtg accatatcct cggagttcaa tatcctcgcc gtcggcctgg tgctgatcgc
    25681 ctttgtcgtg gcggcgccgg aaggcctgtt gggcattcgc ttcaggagac gtgcggcatg
    25741 acgggcagcc ttctgacggt ccgcgatgtc agcaagagtt tcggcccgat caaggcgctg
    25801 ggccctgtcg acctcgacct gatgcctggc gagcgtctcg gcatcatcgg tcccaatggt
    25861 tcaggcaaga cgacactcat caactgcatc agcggattct accgcccgga cagcggctcc
    25921 atcgtcttca aaggagagga catcaccgcc tggcccgctt acaagcgtgc ccgcatcggg
    25981 ctgtcgcgga gctttcaaat tccgcgaccg ttcctcggca tgagcgtctt tgaaaatctt
    26041 ctcgtgccga tggactactg ttccgaaagc gagaacgaga aggaagagat cgagcgcgcc
    26101 ggacaggtgc tcgaatcggt cgggcttgcc gcgcgacgta acgatcccgc agagatgctg
    26161 tcccagctcg agctgcgcaa gctcgaactc gcccgtgcac tggcagctcg acctaaggtc
    26221 ctgatcgcgg acgaggcgat ggcagggctt tcggaggcgg aaatcgacga cgtgctggcg
    26281 ctgcttttca cgctgaacga aaccggtgtc gcggtggtga tgatagaaca tatcatgcat
    26341 gcggtcatgc gcttctctca gcgcgtgatc tgcctcgaaa ccggcacgat catcgccgaa
    26401 ggcaatccgg acgagatcgt cgctagtccc caggttcaga aggtgtattt cggtgaacag
    26461 actcgtcgtt gaaggtttgc atgccggata cggtgccgtc gctgtgctgc gcgacatcag
    26521 cctggaggtt cgcgaaggcg aactcgtcgc cctgcttggc atgaacggca acggcaagag
    26581 cacgctgctc aactgtatta tgggtttcct gcggccgaca tcgggtcgca tctttctgga
    26641 atgggaagga gaaacgatcg aattgtcgag ctcggccgcg cacgagacga tccggcgcgg
    26701 gatcgcctat gtgccggagg gacgacgcct cgcgcccaac ctcacggtgg aggaaaacct
    26761 gttgctggcc ggcgggtcgg gtcacgcaaa ggcaaagctg aaggagaatc tcgacttctg
    26821 ctacgacatg tttccgatcc tcaacgaacg gcgcagacag ctttcatcga cactgagcgg
    26881 aggccagcag cagatgctgg cgatcgccag ggcgctgatg acctcgccca ggatcatgat
    26941 gatcgacgag ccatcggttg gcctcgcgcc gatcgtggtc gagcaagtga tcaacacgat
    27001 tcgcacgctt caggagaata cgaatctcac cattctgatg gccgagcaga gcttcttcca
    27061 ggcgatcgag gttgcctcgc gcgcctacgt gctctcgcac ggacggatca tccacacctt
    27121 cgaccagaag gtcgatcccg cctcgagcga cgaaatccgc aacgcgatga tgggggtcgc
    27181 ctgatccatt cctgctccag cccaggttca aggcgggagg catggttgct cggctggctt
    27241 accgagcaac cggatcagct gaagctgtct tgaatagacg ctgtgcgggg ccgccgattt
    27301 cggccccgac tatgacgcct aaccttggcg tcgaatgaac gctgtgatcg tcaccttctc
    27361 ccatatgtcg gctgccttga agggatcggc attgttgaag cgttcgacgg ccgcacgatc
    27421 tggcgcctcg accagcaata ggctgccgat catggtctgc ccgtcatccg ccaccagcgg
    27481 acccgacatc acgatcttga cgccgaacgc gctggtatcg ctgaggaacg ccttgtgcgc
    27541 gtcgtagttg gcaagacgcg tctcgagcgc gcctggcttg tcgagggcgt ggatgacgaa
    27601 cagcatcatg agctctcccg atttcccttt tttgttgatc atgtgcgatt gaccttcagc
    27661 cacgatcgag cgtgaagtcg tactgcagct cgaaatagct gcggttcatt gcagtgccgt
    27721 cgggcgcggt tcccgccgga cgctcaacga aatcacaaat cagcgaatcc ttgacgccaa
    27781 agacgacgtc ggaatcaaga tactgatcgc ccgcaacgaa gatgtgggtg atgagacgtt
    27841 ggtggcctgg cgcagagatc atgaaatgga cgtgcgccgg gcgccacgga tgccgaccct
    27901 gagctgccag catctcgccg acgggaccgt catacggaat gggataggcg gccggcttga
    27961 tcgtccagaa atgaaaccgc ccccgctcgt cggcgcgcaa gcgcgcccgc atggccagtc
    28021 cgcccacttt gtcgagctgc tggacgtcgt agtgaccgtc gtcgtcggaa tgccatatat
    28081 cgaccacagc gccggcgagc ggcgtgccgt cggtcgaccg gaccgagccg gatacaaaca
    28141 gtggatcgcc ttcaatgccg ggcgagatgt tgtcgccaag ctgatactcg ggcggtgact
    28201 ccacgtagaa cgggccgagc acggttgttt cggtcgcacc atgtgaattg gcgtggttga
    28261 tcgcatcgac gagcatggag atgccgagcg tatcggacag caggatgaac tcctggcgct
    28321 tgtcgtcgca ggcctggccc gtgcgcgtca ggaactggat ggccgccatc cattcctcct
    28381 gggtgggccg gacctcccgc accagtgcat gaagatgcct gacgaccgct tcgctgatct
    28441 ggcgaattct cgggtccggc gaattgcgga cacgttccaa gacggcgtcg gtgatcgtgt
    28501 attcgttgaa gttgcgcatg agattgcctc actccttcct gtcgggctcg cccattccgg
    28561 ccagccattc gatgtgtttc accggagcga tgaaatcgtc ttctcccatg ccccggccga
    28621 tgatggcggc gaaggtctcc cgcgcgagcg ccgtaacagg catcggtacg ttggccgatc
    28681 gggcggcgcc gaggatgagg tccagatcct tggccatctg atagcaggaa aaggtggagg
    28741 tgaaatcgcg ccgcgccagt tgatccacct tgtacttgac catcggcgag gcggcggcac
    28801 tgtcggccag tacgttcaac atgtcctgcc agccgatatt gcccttgcgt gccagggcaa
    28861 ggctctcccc catcatcacc gcggtaaccg ccagcatgag attgacggcg agcttggcat
    28921 agcgcgcctc ctccccctcg ccgaggtaga tctgggcccg tgtgaaggtg gaaagaacgg
    28981 gccggacggc ctcgaacgcg gccttcgggc cggaggcgaa acaggtcagg ttgccggtgt
    29041 gcgcgatcga agcgttgccg gaaatcggca ggcgcagata ggcgataccg gcctcttctg
    29101 ccacgcgggc gacctcggcc gacacctccg ggctcacggt gctgatttct acataaatcg
    29161 cgtcgtccga catggtcttc aggatgccgc gatcgccgag cgcggccgag cgcagcgcgc
    29221 gatcgtcggg cagacaggaa aagaccacct gctttcccgc cgcctcgcca ggatcggtaa
    29281 cgaccgcgat gcccttggcg gaagcggcat cgcgccgctc ctgcgcgatg tcgtatccag
    29341 ctaccgcgtg gcctgcgtca gcaattctcg ccgcgatggg caggcccagc ttgccgagtc
    29401 cgatccagcc tattgccgcc ttgccgctca tcccactcct ccactgatgg ctcctgccaa
    29461 cttgttcccg ttccagccgc cgccctgccg atcttcggac acacgccctg cgcaaccgcc
    29521 gcgccgactg gcgatcacga ggcagggtcc ggcctttcgc ccgcccacgc gcgggagaca
    29581 agacggcgga tagcctcccg ctcgatcgga cgcgggttcc agtaaggatt ttcgatcgcc
    29641 aggtcggccg cgtggtcgat cccctcttca ggcatgccga tctccctcag ggcgcgcgga
    29701 gcgccgagct tgccggccag cgcgtacaga gcctgtgccg ggtcgtccgc gccgagcgcg
    29761 cgcgagagcg cggccatcgc atcaccggcg gcagatgcgt tataggcgac gacgtggggc
    29821 aaaacgatcg tgtgggtttc ggcatgcggc aggtcgaacg cccctcccag gacgtggcac
    29881 agcttgtggt gcagcgacat acccaccgag cccaggcaga taccgcatag ccaggcgccg
    29941 tagagcgcgt ccgaacgcgc ctcgcgatcg gacccgtcgg ccacgacgcc cggaagcgct
    30001 cgcgtgaggg ccgatacggc ctcctccgcc atcagggaaa tcacaggatt gcggtcctcg
    30061 gcatagagcg cctcgacggc atgggcgatc gcgttcatgc ccgaggtggc tgaaagcgca
    30121 ggtggaaggg agagcgtcag atccacgtcg tagaccacca cttcaggcag gaccttcggc
    30181 gtgcgctggg tcttcttcac gccatcggcg gtctcgccca gaatcggcgt catttcggaa
    30241 cccgcatagg tggtaggcac gcaaacctgc ggcagatccg ttcgcagcgc gatcgccttg
    30301 ccaaggccga tcgtcgaccc tccgcctact gcaagcgtgc aatcgacgcc cagttccgaa
    30361 acgaccttca tcgcctcttc cgtcaccgaa acaggcgtgt gcatcatcgc accgtcgaag
    30421 actcccgctg cctttgcgcc gagtcgctcg gcaacgtccc gggcttgacc ggattgttcg
    30481 cgggtggaaa gcaccagcac cctgctacag ccgagccgct cgatctcctc gcccagcacg
    30541 gagagcgtgc ccgagccgaa gacgacacgt gcgggcgcgc tcgtatagac gaacctcttc
    30601 atgtccgccc cttcatgcct accgccactt tcggaaggaa cgcggcaacc ccgcgtatag
    30661 ccggcggcag ccgcgattgt cgccctcgct gaaacggcgt gccaagtgac tttctcctcc
    30721 gcgcactcag agtacagttt caatatacgc gtttaatctg aattaacgaa cgcttgtcaa
    30781 gaaaagagag acaaatacgc tcgcccgcgg ccaacggtgc cgaatttccc gtcccgcgca
    30841 ttttcccgac gcggttcgtg cgttgcgaca gcctcgtcat agtgttcagt agaaatgaac
    30901 gagaccaagc cactctcgtc tgccggcgcg gacggggagt atgggaggaa tgggatcagt
    30961 gggcgactgt gcagctttgc tgatgcgttt tgaagcaggc cgttcggtgg cttctcgtga
    31021 tctatggatg tcggctacgc cgttgcgaca gctattggcg ggatgtcttg cgcccccgag
    31081 aggacggcgc ggcggcttgt gccgacatgt tcagtgctac ggcggcgtga atgagcgctt
    31141 tcaacgcctc ttcatcgatc tcgtcgtcct catggatatc gatggcgcgc ctggtgttgc
    31201 cttcgaggct ggagttgaag aggccggaag ggtcctccag caaggcgccc ctggcaaacg
    31261 tcagttttat tttgtcttta taggtctcgc cggtgcagat gatgccggcg tgctcccaca
    31321 ccggcacgcc cagcatgttc gacggctttc gccatttcac cgtttcgacc acttccgggt
    31381 cggcctgcct gatcaaggca cggagccggg aaagcatctc gccacgccaa tcgtccagct
    31441 cgcggattct tgcatcgatc agtcgagagg gggattcctc gcctggcgtt tcggcgatca
    31501 tgttcaggcc tccttgccga tacaccgccc gctgcgccac ctggtagcat acggcagcac
    31561 gaacatgtag aggccggtga acagcagcag gaagagcggg agcagtggcg aatagaccac
    31621 ccaggagggc ggctctccca atcccatggt gacgaaattg gcgatgaccg tcagcgtaaa
    31681 gacgatcgac agccagcggt ggaactgcct aatccatatg ttccagttca tgggaatttc
    31741 ctctgaaatg agcgggcagc ctcagctcat cttctccaag acctgctcca gggccgcaaa
    31801 gaacctcggc cacccggcct tggcacccct gtagtaggac tgctgatccg cccggaagcc
    31861 tgattgttcc atgcgcagca gggttcccgt atccgtaggt gtgagcgtcc agctgacgac
    31921 gctcgtaaga tccttcgtat cccacgtgta gcacagcgtc ttgttcggct cggctgtctg
    31981 cacccggcag tcgacggtgc cccagtccgc gctgagattg aaacggtgcc ccacgacggg
    32041 actgaagtcg ttcttcatga gccactcttc gatcaggtgc ggttgggtga gcgcgcgcca
    32101 gatcttttcc ggtggaaagg gaaactcacg ttcgacgacg acagagagcg tttcggccga
    32161 catttccctc actggtccat ccttttaagc agatcttgca agcggtcgaa ccggctttcc
    32221 caaaactcgg ccatttgctt cgtccagtcg atcagcggag aaagcgcgcc gggctcgacg
    32281 ctatagtgcg tctggcgacc ttcatgacga tcgcgtacca gccccgcctg tttcaggacc
    32341 ttcagatgtt tcgaaacggc aggctgcgag atccctgccc tggccgtcaa ggcactgact
    32401 gtctgatcgc cctcacggca cagccgctcg aagatagccc ggcgcgtggg atcggcgagc
    32461 gccctgaaaa ggatatcgga gggttgcggc atcgatcaat aaccggttga ctattaaatt
    32521 gattaataac catcgggata tgtatgtgtc aagagtgcag gaaggcggcg gctctgcggt
    32581 tggccctccc gacaagcgac ggcggcgtta cgaagggcgg cacatggtgc tgctcatcga
    32641 aggtccgcaa tttggcgcca gtgagccggc tttccagcga gttcgagcac gccgggcggc
    32701 gacgaagctt ccggtagaac ccgttctgga tccgttggaa gatgaaggtc gaaaaggtgg
    32761 agatctgcgc gccggggaaa ggcaacggca agcaagatcg tcccgtcttc gacacgaaaa
    32821 tgcgtatgat ttcctcggcc tgcgtcgggg gcaagcctcg tatttcgcga cgcttgcacg
    32881 caccccggtc acatgtccgg gaatgcatat gacgagaccc tgtcggcgat gatcgagggc
    32941 gtgaacgtgc gactccatct cggtctttcg aatgggaatt tctgccaacc ttagggtgtc
    33001 tccgctgcat tggcctgcgc agagcctcgc cgatggccgc cgatcagcgc atgggccagt
    33061 tctgcggcac cttgccggag cgtctcggtc gttagaatcc gttctggatg cgctggaaga
    33121 tgaccgtcga aaaggcggag atctgcgcgc cgacaaacgg cccggaaaag gcaacggcaa
    33181 gcaggatcgc cacgatcttg ggcacgaagg taagtgtgat ttcctggacc tgcgtcagcg
    33241 cctgaacgag cgcgatgcca atgccgacca ccatggcgac gatgaccgcc ggacccgagg
    33301 cgacgagcac ggtccagatg gcatattgga cgatgtcgag agcgtcggcc tcgttcacgg
    33361 cagcggcctt cagcggatca cgatcccggg accgactgcg acctcctcgc cggtctcgag
    33421 tatggcgacc agaccgtctt caacaaggcg gacctctgcg acttttcccg tgatctcacc
    33481 atcggatgaa gtgatctcgc ggcctatcag gttccccgcc tgcgacagcg tcgacgactg
    33541 gaggagctga tccagcttgg cattggtccg gactgcctgc tcgacctgcg agaaggaggc
    33601 gagctgcgcc atgtagtcgg tggaatccat ggggtttgtc ggatcctggt tcttcatctg
    33661 ctcgatcagg agcctgagaa aggcctggta gtcgacggtc tgcggcgcct gcgcgctctg
    33721 cgcggcggtg ctcgggcccg ctgcttgggt gggcgatacc tgcatgattt ttactccccg
    33781 accgcgatgg cgcgtggagg ctcgtttctg ccgcgctcgc cgttcatgat ttctgcctcg
    33841 agcggataaa gacggcgaat aagcttcagc gcttcgtaaa tctggccctg gccaaccagc
    33901 cggtcgaggt ccttgagcga agccaggata cgctggtcgg tgaaacaccc caacaggcgc
    33961 gtcagcgtct ctcggaacat ggcgcgcgcc tcttccgtgt cgtccggctg catcagcatc
    34021 acctgcacga tgaaatagag ctggcgcagc ggggtgtcag cgtcttccac ctgaagaaca
    34081 tgctgttcga gcaggaactg cacgtcgttc aggaactcca gcgagacctt gcggtcgggg
    34141 cggatcaccg cgccgttgat atagatcttc tcgttcgcct tgagcgagat cttgagcgta
    34201 ttagtcattg catgccgtca cggatgatct gcgatacctc gatcaggccg tcgaaattgt
    34261 ccgatcgccc ctggcgcacc tcctcggcct cgcgcagcag ccagagaccg atagaaatca
    34321 gattcgcgcg cagctctttc ggcagcgcgt tgtcggggtt gccgaggtct tccacgaaaa
    34381 ccgtccacac acgattcaag aagtgtatgg cctccaccgc ctccctcgac tggctcccct
    34441 tttctctcgc ggcgatcaga agctcgatcg agcgcgaaag cacctggcgc tcgcggtcct
    34501 tggcgtcggc catggagtcg gtttgaattt cactgtagga gaactggtac atcggtgttc
    34561 cgtgttctgg tcttgtccgc tacgtcaggt atctgacgag gctgagctgc tggatgcgcg
    34621 aggttagcgt gtaggagatc tcgatctgcg tcatgagctc gttcactcga gtcgctgcct
    34681 caaacgggtc gacgccttcg agatcgttga tgaaggactt gaagaggtcg atctgggagg
    34741 agatgcggtc ggtggcatcg ctgacacggt tttcggcgat gccggtgcga ccctggaggc
    34801 cggccatgtc cgcaataccc tcgcccacga tttctgcggc acgctgaatc aatgcatccc
    34861 tggcgctctc gctgagcggt gccttcagca agtcggagat tgcaaccgcg gccatcagga
    34921 gcttcttgaa gccctcttcg ttggctgtga cagaggtttc cgctgtctcg ttgagcgtga
    34981 tccggctcac gatcggctgg tcggccgcgt cggaccagtt gtcggtccag ttggcgcctt
    35041 ggaactgggc cgcgatctcg ttgtccagga agctggtgat ttcagcggcg gtgatgctgg
    35101 ccgccgcggg atcagattgc gcgaacccga agcgcgcctg gaaggcagca tcgaaagcag
    35161 ccttgctgtc ggacggtgga ttgtcggtaa atggcttaat cggcttggca tcggtgttga
    35221 tgccggcgaa cagatattcg ccattaaaac tcgtgttcag gatgccggtc accgcttcca
    35281 gcgttctgac cgcctcgtga cgcaccacgt ccgtgtcgac cgagccggag gctgctgctg
    35341 tcaaagcgct gagcagatcc tgtgcgttat cggtaatctg gttcagagcc gcctgggtgg
    35401 ctgccagccg attggccgcc agactgttgg aatcgacgag cccgctgagg cgctcgatat
    35461 cacggtccat ggagactgtg cggctgacgc cggcgccgag cttgacaccg ggatcagtga
    35521 cacggccggt aaccgcctcc tgtcgcttct ccgcaagctc cgcctgcatg cgcatgagct
    35581 ggtagcgcat gacctgtgcg actgagtgcg ttgaaataag ggaggtcttc attgcaccag
    35641 gcctatccgg tcgcagccag caatgccgcc agcatctcgt cgaccacgcg gatcatgcgc
    35701 gcggaagcct cgtaagaatg ctcgagatcg agaaggagcg acatctcctc gtcgacatta
    35761 acgcccgtcg cgttggaaag cgctgtggcg gtacgcgtcg aaagggctcc cttcgcctcg
    35821 ttgccgtacg cggcgtcctg gcgcaggttt tccagccagc cgaccgcagc actcgtatag
    35881 gtgccgaggc tcgcgctgac ctcgaggccg gcagccgggt cgaagttcat cggctcgtcc
    35941 agcttctcgc cataggtccg cagaagctcc gaataggagg ctgcggcagg tggaccgttg
    36001 tgtacgtagg cggcaccgtt ggcaccgcca tcgcgcaacc gcatggggtc gccgccctgg
    36061 ctggaatcca tcgccggatt gatgctgatt tctgctccaa gcccgtcgac cagcgtgcca
    36121 tcggcaggga tcgcaggccc cccggaccag gtaaagagac ccgccgcatc cggctgtgtg
    36181 ccagaagggt cggtctcggc gaagacggcg atcagcccgc gcgcgatctc gtcgatctgg
    36241 cgctgcatgg ctggcgccac ctcgtcgcga agctggagct gcgcggcgat cgagcccgaa
    36301 gcggtagtgt ttccgccgct gccgcccttg agcgccacgc cgtcgatata gaccgagttg
    36361 ccgcccttgc ctgcgctgaa gacggcggtc ggctcgaagg tgaccggtcg cggcaccgtc
    36421 tcgaacaaca ccgcgccggc cgtcgtcgtc agcatcacgt cgttgtcggc gcgcttgatg
    36481 gtagagaccg gcacgtattc ggcgatcttc ttcagaagcg cgtcgcgccg gtcgagtgcg
    36541 tccgatacgt cgcggcccgc gcgcgtgccc gagatgatct cgttgttgac ctctttgaag
    36601 tcggccagca gaccgttgag cttgtcgaca gaggcggcga tctgctggtc ggtgtcgcgc
    36661 cggaacgact ggatcgccgc actgccttga ttgaggctgc gcaccatctg ccgcgcggcc
    36721 tctaccgcgc tttcggcgag cgtgctgttg gaaggggagg cggcgtagag ctgcagcgcc
    36781 tcctgtaatt tgccaagcat gacggcggca gaattggcat tgtcgacgcc gttgacgtcg
    36841 agctgcagcg agtccaagcc cgccgaaacc atcttctgcc cctgccagga cgagatcgcc
    36901 tgcaggttct ggcggaaaag cacttcgttc gtcgcccggc ggatttccgc cacgcgggcg
    36961 cccggcgcca tgctggccag caccgcgcta cgccgcgcat agtccgggtt cgacgcctcc
    37021 gacaggttcc gggagacgat gctcgtctgc cgcgaggtgt tgaaaagtgc ggactgtgcg
    37081 atgctcagtg cggaagtcag agacatagga ccatgatccc gggcgtgcgc catcacttac
    37141 cttttcaggt tcaccagaag atccatgagc tccgagccgg tctggaagac cttcgagttg
    37201 gcggtataat tacgctgggc ttggatcatg tccgtcagct cctgcgcgat atcgacattc
    37261 gagttctcca atgcgccgga gaccatctgc cccatcccgc cctcgccggc aaagccgatg
    37321 cgcacaccac ccgatgacgc gctctcggcg aagacgtttc cggacaactt ctgcagattg
    37381 tccgggctcg gcacgtcggc aagcggaacg cggtaaagcg cgcggaacga gccgtcgtcg
    37441 tactgcgcat agaccgtacc gtctccggcg atctcgaccc tgtcgatcgc gctcggcggg
    37501 ttgccatcga catccgcgcc gaagactgtg aaatcggcgg aaagctcgct catggctgac
    37561 agatcgagcg tcagcgtctg cccgccgggt accgatatgg cgacgttggt gtcgctcgtg
    37621 cctgccagct tgccgttgga cgagtcgaat tcgagttccg ctgtccccaa cgggggtgaa
    37681 ccgggtgtgc catacgggaa ggggccaccg ggggtcgcct cgtcccgatc gaagacggcg
    37741 acatcccacc tattggcccc ggtcttggta aaatagacgt cgagcagctt ctgcccgccg
    37801 gtattgtcat agacgacgag cgaggtcttg gcagagtatt gcgatgtggc cgaattgtcc
    37861 gagggtgaat cggctgtaac aactgccgcg tcctcgggca gatttgcgga aagatagccg
    37921 gtcgccgaag gcgatgccgt gagcgcgctc tgcccgatga cgaccggctc cagcccgtcg
    37981 tagccgtttg cggtcgccgc cggtatgccg ttggcgtagc tgtatcccat cagcgtgtag
    38041 cccgcagcgt ttacgagctt gccctgcccg tccggcacaa aggagccggc gcgcgtcagg
    38101 aagggctgac cactggaatc ctgcacgatg aagaagccgc tgccgctgat ggcgaggtcg
    38161 gtcgtcgagg tggtgtattg cagcacgccc tggctcgaga tggccgtgcg cactgcggtc
    38221 gtcacaccgc ccgaattgta ggagcccgcg gtgctgggca cgaccagcga ggagaattcc
    38281 gtactggagc gcttgtagcc gctggtgctg gaattggcga tgttgtcagc caccgtcgac
    38341 aaacggttgg cttgggcaga catgccggac acgccggtac gcatcatgcc gtataggctc
    38401 atcgggcttc tcctcgtatt ccacgccgat gccgacgatc gtagtgtccg ggacttgcgt
    38461 gaaactgtcg gcgggaagag ccgggctgcc ctggcatcac gtctgcgggc acctcacgca
    38521 atcagccggt atccgagata acgtttggac tcgatcggat cgaagccaag tctttcgcgc
    38581 aatttcttgc gcagcttgct gat
//
LOCUS       DASVDU010000229         6443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_14088, whole genome shotgun sequence.
ACCESSION   DASVDU010000229 DASVDU010000000
VERSION     DASVDU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14088"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VF226_14800"
     CDS             <1..619
                     /locus_tag="VF226_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7075306.1"
                     /translation="RGAMAGMDHGSAGAAGHSGMSTRDPANAPADMKVGVGVDMIAPM
                     PQDRTGHPGLGLENVGHRVLTYRDLVSLAPNPDPRPPSRSLEIHLTGNMERFMWSFDG
                     RKFSELVEPIRFARNERVRVTLVNDTMMQHPIHLHGHFFEVVNGNPGNHPLKHTVNVL
                     PGSSVTFDLTADAPGDWAFHCHLLFHMHAGMFNVVTVRPLDGEVT"
     gene            1120..1749
                     /locus_tag="VF226_14805"
     CDS             1120..1749
                     /locus_tag="VF226_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HEX7075307.1"
                     /translation="MRTYRLLADQLEARRRDGRSGYLWDAQGWYGGDINKLWIKTEGE
                     DASGEESEEAEIQALWSRAVTPWFDFQAGVRYDLESAPERRHLVVGMQGLVPYFFEID
                     GAAFFSDEGDITAQLEAEYDQFITQRLILQPRIELDLAAQDIPELGIGSGLSSAEFGL
                     RLRYEFARELAPYVGIEYERAVGGTADFVRAEGEEVGEWSVLLGVRAWF"
     gene            1772..2149
                     /locus_tag="VF226_14810"
     CDS             1772..2149
                     /locus_tag="VF226_14810"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEX7075308.1"
                     /translation="MKSLICVAALLVMTSGVAYAHAELSGSMPADQAVLEAAPKELML
                     HFTEEVRLTTLSIRAPGGEKQDLGPLPASMGRDFTVAAPSLEAGDYVVSWRALSEDTH
                     VMTGEFKFTVQGGNASHAQHSAH"
     gene            2239..3096
                     /locus_tag="VF226_14815"
     CDS             2239..3096
                     /locus_tag="VF226_14815"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX7075309.1"
                     /translation="MADALAISLRALSFIAALQAAGAPLFLWLFADRIGEARRPIAVL
                     ARRSAVAGLLLAVGHQLVEPARVVGALAGIGDGAMQALLLASDAGTATIVRVFGLALI
                     AFASLSRRFGAAAAVAGGTLIVTSFTFMGHTAASDLRWLLIVLLILHLLIVALWFGSL
                     LPLYLAGKYEDIRTNGALVRRFSALAIRLVPVIFLAGLGIAVVLVPGLASLGTPYGTL
                     LLSKIGGFALLMGLAALNKWRLGPAIEGGDSGALRALQRSVLAEWVLIAAVLAATATM
                     TALFAPTAH"
     gene            3024..4235
                     /locus_tag="VF226_14820"
     CDS             3024..4235
                     /locus_tag="VF226_14820"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEX7075310.1"
                     /translation="MGIDRGCIGRNRDNDGVVRADGALAKRHVQLQAETRAAMTNKAF
                     RWSRALHAWGGATLALLVLLNTATGTLLVWKNEYVRLTVPEARVEFEPTAAALARIAS
                     AVEAQFDNDEILLIQFGTQQFPLTKITLTDARYAYCDIRGNIVDEWVQNERFEEWLYD
                     LHHRLLLENTGLTIVGLMGLALIILVVAGVISFWPLRRAFPRGLWPKSIARPHLLNAH
                     RNVGIIEALPFLLMLVTGVVLAFPEQAEKLLLESVRSSEEYGAGFAEKVDAISGGNSG
                     DWLPAMRRALATFPGAKIRSAQVPNTFSPYRIIGLQQSGGLHPQGLSKVYIDAEGGYM
                     DVRIDDTKLPLVERLYSASYPLHTGKVDSMSYKLFLTVSGLLVSALSVLGLVCFIGKV
                     KRQRGAAPDGI"
     gene            complement(4232..6049)
                     /locus_tag="VF226_14825"
     CDS             complement(4232..6049)
                     /locus_tag="VF226_14825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HEX7075311.1"
                     /translation="MVGRRTLIVGGVTLGGSAVLAPLLPAWARNGTPGIAPDMQTLSG
                     EQIDLTIGRSPFTLGGRTGEAVTMNGTVPAPLIRLREGQTVRIAVTNTLDEDASIHWH
                     GVLVPFTMDGVPGVSFPGIRPGETFIYEFPIRHPGTFWYHSHSGFHESLGQYGPLIFD
                     PRDADPVEYDREHVIVLADWSFLRPHEIVAKLKKAGAEYFNFQRQTLAGLLREGDPEQ
                     SMPLENRLMWGRMRMQPTDIADVTGSTYTYLVNGHGPNENWTGLFRPGERVRLRFINS
                     AAMSIFNVRIPGLPMIVVQADGNNVRPVTVDEFQISVAETYDVIVQPMEDRAFTLVAE
                     SVDRSGMGRGTLAPRMGMAAEVPPLRPRPTLGMDDMGMAAMGHESMASMADGSMTDAQ
                     QQAGAAMDHGGSDGSGAMSMRGMKMRDESLARPDMKVGVGVDMIAMNPQDAAGKPGLG
                     LENVGHKVLVYTDLVALDPNPDTRPPSRSMEIHLTGNMERFMWSFDGRKFSEIVEPIR
                     FARNERVRVTLVNDTMMQHPIHLHGHFFEVVNGHSGRYPRKHTVNVLPGSKISFDLTA
                     DAPGDWAFHCHLNFHMHAGMFNVVTVRPLDDETASRSSG"
BASE COUNT         1139 a         1951 c         2101 g         1252 t
ORIGIN      
        1 ccgtggcgcc atggccggga tggatcacgg ctccgcggga gcagccggac atagcggcat
       61 gagcacgcgc gatcctgcta acgcgcccgc ggacatgaag gtcggcgtgg gcgtggacat
      121 gatcgctccg atgccacagg accgcaccgg ccatccgggg ctcggtctcg agaacgtcgg
      181 ccacagggtg ttgacgtatc gcgatctcgt ttcgctcgcc cctaatccgg atccgcgccc
      241 accgtcgcga tcgctcgaaa ttcacctcac tggcaacatg gagcgcttca tgtggtcgtt
      301 cgacgggagg aagttcagtg agctcgtgga gccgatccgg ttcgcgcgca acgagcgcgt
      361 gcgcgtcacg ctcgtcaacg acacgatgat gcagcacccg atccacctgc acggtcactt
      421 cttcgaagtc gtcaacggaa accctgggaa tcatcccctc aagcatacgg tcaacgtact
      481 tcccggtagc agcgtcacgt tcgacttgac cgccgatgcg ccgggcgatt gggcctttca
      541 ctgccatctg ctcttccaca tgcacgcggg catgttcaac gtcgtcacgg tccgcccgct
      601 tgacggagaa gtgacatgaa ggctttcctg atcgcattgc ccatttgggc aacgggagct
      661 tcggtccttg cccaaccaca tcagggccat accgaagacg agcgacgcgt cggatccgac
      721 gctgcgccga cggcttcgcc tccgccttct gccccggcgt ctggacccgg ccaacacacg
      781 ggtcacggtg aaaggccaca ggcggatcac gctgaaacgt cgcccgcgca ggccggcgcg
      841 gccgatgagc acgcaggtca ttctgagacc gccgactccg agactccgac cgaagctcat
      901 ccggaacacg acgcggttgc gtccgaaccg gatgctgaac cgtctcgggc cgacccgcat
      961 gcagcacacg aagccgctcc ggcggacacg gctccgacac cgcctgaagc gccgccgccc
     1021 ccagccgcgt tcgagggacc gcggcacgcc gccgagacgg tcttcgatcc ggccgtgatg
     1081 gcctccgctc gagagctgct tcgtgccgag caaggcgcta tgaggacgta caggctgttg
     1141 gccgatcaac tggaggcgcg ccgacgcgat ggccggagcg gctacctgtg ggatgcacag
     1201 ggctggtacg gcggcgacat caacaagctt tggattaaga ctgagggcga ggacgcgtcc
     1261 ggcgaggagt cggaagaagc agagattcag gcgctgtgga gccgggccgt gacgccttgg
     1321 ttcgactttc aggcgggtgt tcgttacgac ctcgagtcgg ccccggagcg ccgccatctg
     1381 gtcgtgggga tgcagggtct cgtgccttac tttttcgaga tcgacggtgc ggcgtttttt
     1441 tccgacgaag gagatatcac ggcacagctc gaagccgaat acgatcaatt catcacgcag
     1501 aggttgattc tgcaaccgcg catcgaactc gacctggcgg cacaggacat tccggagctc
     1561 gggatcggct cggggctttc gtcggcggag tttggactgc gccttcgcta tgagttcgcg
     1621 cgggagctgg caccctatgt cggcatcgag tacgagcgcg ccgtcggcgg cacggcggac
     1681 ttcgtacgag ccgaaggcga ggaagttgga gaatggagtg ttctgctcgg agtaagagcg
     1741 tggttctagc gttcccttga aggagactga gatgaagagc ttgatttgcg tagctgcgtt
     1801 gttggttatg acgagtggcg ttgcctacgc gcacgccgag ttgtccggct ccatgccggc
     1861 cgaccaagcc gtgctcgaag cggcgccgaa ggagctgatg ctgcatttca ccgaagaggt
     1921 cagattgacc acgctgtcga ttcgagcgcc gggcggcgaa aagcaggatc tcgggccgct
     1981 cccggcgtcg atgggccgcg attttacggt agccgcgccg tcgctcgagg ccggcgacta
     2041 tgtcgtgagc tggcgtgcgc tgtcggagga cacgcacgtg atgaccggtg agttcaagtt
     2101 cacggttcaa ggcggcaacg catcgcatgc gcagcactcg gctcattgag ccgaggaggc
     2161 gcttcggccg ggccggcatg cgtggacggc cgcgtgccag aacggtctct gcatcgtcag
     2221 ccacggtgat cggtcaccgt ggctgacgcg ctcgcaatca gcctgcgggc gctgagtttc
     2281 atcgccgcgc tccaggcggc cggcgcgcct ctgttccttt ggctcttcgc tgatcgcatc
     2341 ggcgaggcca ggcggccgat cgccgtgctc gcgcgccgct ctgccgttgc agggttgctg
     2401 ttggccgtcg gtcatcagct cgtcgagccg gcgcgagtgg ttggtgcgct cgctggcatc
     2461 ggggacggcg cgatgcaggc actcctgctc gcttcggatg cggggacggc aacgatcgtg
     2521 cgcgtgttcg ggcttgcgct gatcgcattc gccagtctgt cgaggcgctt cggtgcggct
     2581 gcggcagtcg ccggcggaac gctgatcgtg acttcgttca cgttcatggg tcacacggcg
     2641 gcgagcgatt tacggtggct gttgatcgtt ctgctgatac tgcacctgct gatcgtcgcg
     2701 ctttggttcg gttccctgct gccgctctat cttgccggca agtatgagga cattcgcacg
     2761 aatggtgcgc tcgttaggcg gttctcggcg ctcgcgatcc ggctcgttcc ggtgatcttc
     2821 ctcgcggggc tcgggatagc agtcgtgctc gtgccgggcc tcgcgagcct cggaacgccc
     2881 tacgggacgc tgttgctcag caagatcggc ggattcgcgc tgctgatggg acttgccgca
     2941 ctcaacaaat ggcggctcgg tccggcgatc gaaggaggag attccggcgc tcttcgcgct
     3001 cttcagcggt cggtgctggc cgaatgggta ttgatcgcgg ctgtattggc cgcaaccgcg
     3061 acaatgacgg cgttgttcgc gccgacggcg cactagcgaa acgccatgtc caattgcaag
     3121 ccgagactcg cgcagcaatg accaacaaag cgttcaggtg gtcgagagcc ttacatgcct
     3181 ggggcggcgc gactttggct ctgctggtgc tgctcaacac cgcgacgggc acgctgctgg
     3241 tctggaagaa cgagtacgtc cggctgactg ttccggaggc cagggtagag ttcgagccca
     3301 cggcggcggc tctcgcaagg atcgcatcgg ccgtcgaggc gcagttcgac aatgacgaaa
     3361 tcctcctcat ccagtttggg acgcagcagt ttcctctgac caagatcact ctgaccgacg
     3421 cgcgctacgc ctactgcgac atcagaggca atattgtgga cgagtgggtg cagaacgagc
     3481 gcttcgagga atggctgtac gacctgcacc atcgtctgct gctcgagaac accgggttga
     3541 ccatcgtcgg cctcatgggt cttgcactga tcattctggt agtcgccggc gtgatttcat
     3601 tttggccttt gcgcagggcg tttccccgag gcctatggcc gaaaagcatc gcgcgaccgc
     3661 atctgctcaa cgcgcatcgc aacgtcggca tcatcgaggc attgccgttt ctcttgatgc
     3721 tggtgaccgg cgtcgtgctg gcttttccag agcaggcaga gaaattgctg ttggaatccg
     3781 tcaggagcag cgaggagtac ggcgcgggat tcgcggagaa ggtcgatgcg atctccggcg
     3841 gcaattcggg cgactggctg ccggcgatgc gaagggcgct cgccactttt ccgggtgcga
     3901 agattcgttc ggctcaggtg ccgaatacgt tctcaccgta ccgcatcatc ggactgcaac
     3961 agtccggtgg actgcatccc cagggtctca gcaaggtcta tattgatgcc gaaggcggct
     4021 atatggacgt tagaattgat gacacgaaac tgccgctcgt ggagcgcctg tacagcgcca
     4081 gctacccatt gcataccggc aaggtggata gtatgtcgta caaactgttt ctcacggtca
     4141 gcggcctgct ggtctcggcc ctaagtgtgc tcggactcgt atgcttcatc ggaaaggtga
     4201 aacggcaacg aggggcggcg cctgatggca tctagccgga gctgcgggac gcggtctcgt
     4261 catcgagcgg ccggacagtg acgacgttga acatgcccgc gtgcatgtga aagttgaggt
     4321 ggcagtgaaa ggcccaatcg cccggggcgt cggccgtcaa gtcgaagctg atcttgcttc
     4381 ctggcaagac gttgacagtg tgctttcttg ggtaacggcc ggaatgcccg ttcaccactt
     4441 cgaagaaatg gccgtgcagg tggatcgggt gctgcatcat cgtgtcgttg acgagcgtca
     4501 cgcgcacgcg ctcgttgcgc gcgaaccgga tcggctcgac gatctcactg aacttcctcc
     4561 cgtcgaacga ccacatgaag cgctccatgt tgccggtcag gtggatctcc atggagcgtg
     4621 aaggcggtct cgtatcaggg ttcgggtcga gcgcgacgag gtcggtgtag accagtacct
     4681 tgtggccgac gttttcgagc ccgaggcctg gtttccccgc ggcatcctgt ggattcatgg
     4741 cgatcatgtc cacgccgacg ccaaccttca tgtccggccg cgcgagcgat tcgtcgcgca
     4801 tcttcatgcc gcgcatactc atagcgccgc tgccgtcgct cccgccgtga tccatcgcgg
     4861 ctccggcttg ctgctgcgcg tcggtcattg atccatcggc catgctcgcc atcgactcat
     4921 ggcccattgc agccatgccc atgtcatcca tcccgagggt cggacgggga cggagcggcg
     4981 gcacttcggc cgccatcccc atgcgcggtg cgagtgtccc gcgacccatg ccggagcggt
     5041 cgacggattc ggcaacgagg gtaaacgcac gatcttccat gggttggacg atcacgtcgt
     5101 acgtttcggc aacggagatc tggaattcat ccacggtcac cggccgcacg ttgttgccgt
     5161 ccgcctgcac cacaatcata ggcagccccg gaatgcgcac attgaagatg gacatcgccg
     5221 ccgaattgat gaagcgcagc cgcacccgct ccccgggtcg aaacagccct gtccagttct
     5281 cgttcgggcc gtggccgttg acgaggtagg tgtaagtgga gcctgtgacg tcagcgatgt
     5341 ccgtaggctg catgcgcatt cggccccaca ttagccggtt ctcgaggggc atgctctgct
     5401 cgggatctcc ttccctcagc aggcctgcga gggtttgccg ttggaaattg aagtactcag
     5461 cgccggcctt cttgagcttc gcaacgattt catgcgggcg caggaagctc cagtctgcta
     5521 gcacgatgac gtgctcgcgg tcgtattcca cgggatcagc atccctggga tcgaaaatca
     5581 acggcccgta ttgcccgagc gactcgtgaa agccggagtg gctgtgatac cagaaggtgc
     5641 cgggatgccg aatcgggaac tcgtagatga aagtttcgcc gggacggata cccgggaagc
     5701 taacccccgg tactccgtcc atcgtgaagg ggaccaagac gccgtgccaa tgaatcgatg
     5761 cgtcctcatc gagcgtgttc gtaacggcga ttcgaacggt ctggccttcc ctgagtcgga
     5821 tcagcggggc agggaccgtt ccgttcatcg tgaccgcctc acccgtgcgg ccgccgagcg
     5881 taaacggact gcggccgatc gtcaggtcga tctgctctcc cgagagcgtt tgcatgtcgg
     5941 gtgcgattcc cggtgtgccg ttgcgtgccc aggcggggag caagggggcg agcacggccg
     6001 accctccgag cgtgacgcct cccacgatca aagtgcggcg gccgaccatg agcagttcct
     6061 ccctgcaatc aggagtggtg gcggtcaagc tccgtgtcga tatgcgggtc gccgtcggcg
     6121 cccgtagtga ctgactgtct gccgacagtc agagaacgac cgatccggcg gagcatctcg
     6181 ctcgtccggg gataagcaat tgtcgttcca gatggggatt tgccgttcaa cgagagccgg
     6241 agcagaggtt cagccgcagc gccatcaacg cccgcttgac cttcccatca tggaaaggtg
     6301 gatcattcac ggtaccgatt cccgggtacg gcgcatcatc aaacgtggga gccgaacatg
     6361 aggcagacga gcaggatacc ttcccgagtc gagctcgagg tggcgaaagg tattgggacg
     6421 catagcatcg gcgaggcggc cga
//
LOCUS       DASVDU010000230         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_612313, whole genome shotgun sequence.
ACCESSION   DASVDU010000230 DASVDU010000000
VERSION     DASVDU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612313"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..738)
                     /gene="pyrH"
                     /locus_tag="VF226_14830"
     CDS             complement(25..738)
                     /gene="pyrH"
                     /locus_tag="VF226_14830"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7075312.1"
                     /translation="MVPSYKRILLKLSGEALMGSDPYGINRQTIESIVAEIAQVAGLG
                     VEIGVVIGGGNIFRGVAPGASGMDRATADYMGMLATVMNALALQDAMRHAGLASRVQS
                     ALHVEQIVEPYIRGKAIRYLEEGKIVIFAAGTGNPFFTTDTAAALRGSEMGAEIVIKA
                     TKVDGVYTADPKKDPSAKRYGRISFDEAIARNLKVMDATALTLCRDQKLPINVFSIFK
                     KGALRRVVLGEDEGTLVHV"
     gene            complement(773..1651)
                     /gene="tsf"
                     /locus_tag="VF226_14835"
     CDS             complement(773..1651)
                     /gene="tsf"
                     /locus_tag="VF226_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7075313.1"
                     /translation="MAEITASLVKELREMTGLGMMECKKALSEARGDLRKAEELLRIR
                     SGAKASKAAGRIAAEGVVGAFISADARLAALVEINCETDFVAKNEDFLSFANGVAELV
                     ARADPRDVEALASLVLDGAKVEERRQSLVQKIGENISIRRFERLQTASRLALYLHGAK
                     IGVIVDYEGSEQVGKDLAMHIAFAKPAYLAKTDVPADVIAREREILTARAKESGKPDG
                     IVAKMVEGGVNKFLNEIALLGQPFVKDDKQSIEKMLGTHKAKVQGYRYIVVGEGIERK
                     TNDFAAEVQAQAGLAR"
     gene            complement(1763..2500)
                     /gene="rpsB"
                     /locus_tag="VF226_14840"
     CDS             complement(1763..2500)
                     /gene="rpsB"
                     /locus_tag="VF226_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7075314.1"
                     /translation="MRQMLEAGVHFGHQTRYWNPKMAPFIFGHRNKIHIINLETTLAM
                     YQDALKYIRRLAANKGNLLFVGTKRQAREIIREEAIRCGMPFVDHRWLGGMLTNFKTV
                     KQSIKRLKEMEQMAQDGTFERMSKKEALGLQRELEKLNRSLGGIKDLGSLPDALFVID
                     VGYQKGAIAEAVKLGIPVVGVVDTNHSPEGIDYVIPGNDDSSRAIRLYARGVADAVLE
                     GRSQSVEEIVEASRDEFVEVDDDASEQ"
     gene            2727..3527
                     /gene="map"
                     /locus_tag="VF226_14845"
     CDS             2727..3527
                     /gene="map"
                     /locus_tag="VF226_14845"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7075315.1"
                     /translation="MAVTRKTPDEIAKMRVAGRLASEVLDFIAPHVRHGVSTGKLDAL
                     CHDYMVKVQDTVPAPLNYAPPGYKPYPKSICTSVNHQVCHGIPGDRVLKSGDILNIDV
                     TVIKDGFHGDSSRMFYVGEPGVQARRLVEVTYECMWLGIMQVRPGGRLGDVGAAIQAN
                     AEQNGFSVVREFCGHGIGRNFHEDPQVLHYGKPGTGLVLEPGMTFTVEPMINAGKAAI
                     RELADGWTIVTKDHSLSAQWEHTVLVTESGYQVLTVSSNSPPPPAFAA"
     gene            3531..>4729
                     /locus_tag="VF226_14850"
     CDS             3531..>4729
                     /locus_tag="VF226_14850"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX7075316.1"
                     /translation="MAATQPARPGTTVSQIRAQLQAARGALREAYLRRARPHALLRGH
                     SNLVDRIIKAIWRESQPAAGAALLATGGYGRAELFPFSDIDVLILLAREPSSDERSRL
                     EFLVGQLWDVGLEVGHSVRTIAACLAAAEADITVRTTLLEARFLAGSRTLHRKLEAAL
                     HQALDPASFYKAKKLEQEQRHAKHQDTPYSLEPNLKEAPGGLRDLHVIQWIARAAGIG
                     SGWRDLETSGLIQHDEMRQLSRSESVLQNLRIRLHYLAGRREDRLVFDLQNALADQLG
                     DSETPTRRASEVMMQRYYRTAKTITQLNTILMQNLGARLFPKSEVELQPLNERFRARG
                     ELLEACDEELFEREPLAVLESFLLMQQHHELQGMTAPTLRALWRGRRRIDSRFRRDPV
                     ARLMFLQV"
BASE COUNT          876 a         1547 c         1327 g          979 t
ORIGIN      
        1 atcatgtcgg cctcctttgc agactcacac gtgcactagc gtgccctcgt cttcgccgag
       61 aacaacgcgc ctcagcgcgc ctttcttgaa gatactgaaa acgttgatcg ggagcttctg
      121 gtcgcgacag agcgtgagcg cagtcgcatc cattaccttg agatttcgcg cgatcgcctc
      181 gtcgaagctg attcggccat atcgctttgc cgagggatcc ttcttcggat cggcggtgta
      241 gacgccgtcc accttggtcg ccttgataac gatttccgct cccatctcgc ttccgcgcaa
      301 ggcggccgcc gtgtcggtag tgaagaacgg attaccggtg cccgccgcga aaatcacgat
      361 cttgccttct tccaggtaac gaatcgcctt gccccggatg tacggctcga cgatttgctc
      421 aacatgcagc gcggactgaa ctctgctcgc cagcccggcg tggcgcatgg catcctgcaa
      481 tgccagcgcg ttcatgacag tggcgagcat tcccatgtaa tccgcggtag cgcgatccat
      541 acccgaggca cccggcgcaa cgccgcggaa gatgttgccg ccaccgatga cgaccccgat
      601 ttctacgccc agaccggcca cttgcgcgat ctcggcgaca atactttcga tcgtctggcg
      661 gttgatcccg taaggatcgc tccccatcag cgcttcgccg gacagtttga gcaggatgcg
      721 cttatagctg ggtaccaagg ctctgattct ccggcgcggt ggctatgtct ggctagcgtg
      781 ccagtcccgc ctgtgcctgg acttccgccg cgaagtcatt cgtctttcgt tcgatccctt
      841 ctccaaccac gatatacctg tacccttgca ccttggcttt gtgagtgccc agcatcttct
      901 cgatgctctg cttgtcgtcc tttacgaagg gctgtccgag caaggcaatc tcgttgagga
      961 atttgttgac cccgccttcc accatcttgg ccacgattcc atccggtttg ccggattctt
     1021 tggctctcgc cgtcaggatc tcgcgctcgc gggcgatcac atccgccggc acgtcggtct
     1081 tggcgagata ggccggcttg gcgaacgcaa tatgcatcgc gagatcctta ccgacctgtt
     1141 ccgaaccttc gtagtcgacg atgacgccaa tcttggcgcc atgcagatag agcgctaggc
     1201 gtgacgcggt ctgaagccgc tcaaaccggc gaatagagat gttttcgccg atcttttgta
     1261 ccaagctttg cctgcgctcc tctacctttg cgccatcgag aacaagcgac gccagcgctt
     1321 cgacgtccct tggatccgcc ctcgctacca gctctgccac tccgttggcg aaagacagga
     1381 aatcctcgtt cttcgccacg aagtccgttt cgcagttgat ctccaccagc gccgccaaac
     1441 gggcatccgc ggaaatgaac gcacccacga cgccttccgc cgcaattcgc cccgccgcct
     1501 tgctcgcctt ggccccgctc cttatgcgca aaagctcttc cgctttgcgc aggtcgcccc
     1561 tggcttcgct gagcgccttc ttgcactcca tcattccgag cccagtcatt tcccggagtt
     1621 ctttcaccag gcttgcggtg atctctgcca ttgctcgctg ctccttgcca ggggctgcca
     1681 agccccgtat cggtcgaatt cacatttcag gttgaaaaaa ggggccatcg gccccctttt
     1741 catgccccgg gctacgcccg catcattgct cgcttgcgtc gtcgtccact tcgacgaatt
     1801 cgtcgcgcga cgcctcgaca atctcttcca cggattgaga gcgtccttcg aggacggcat
     1861 cggcgactcc ccgtgcatac agcctgatcg cgcggctgga atcgtcgttg cccggaatga
     1921 cgtagtcgat cccttccggc gaatgattgg tatcgaccac ccccacgacg ggaatgccga
     1981 gtttcaccgc ctccgcgatc gcgccctttt gatagcccac gtcgatcacg aacaacgcgt
     2041 caggcaggct ccccaaatcc ttgatcccgc ccagagagcg attgagtttt tccagttctc
     2101 gctgcagccc cagcgcttcc ttcttggaca tccgctcgaa ggtgccgtcc tgcgccattt
     2161 gctccatctc cttcaagcgc ttgatcgact gcttgacggt cttgaagttc gtcagcatcc
     2221 cgccgagcca acggtgatcg acgaaaggca tgccgcagcg aatcgcctcc tctcggatga
     2281 tttcgcgcgc ctgacgcttg gtgccgacga acagcaaatt gcccttgttg gccgccagac
     2341 gccggatgta cttgagcgcg tcctggtaca ttgccagggt cgtctcaaga ttgatgatgt
     2401 ggatcttgtt gcgatgcccg aagatgaatg gcgccatctt cgggttccag tatcgagttt
     2461 ggtgcccaaa atggacgccc gcctcgagca tttgacgcat ggtcactgac acaatttgct
     2521 cctttagggt taagtctgcc gcgcggccga actgtcgacc cggcaagagc cgggcaccct
     2581 aaccagccgg gcaggaaatt tggtcatggc actcctcttc gaagcgaccc cgaccagcct
     2641 aaaattctag cataatcagg tatcaacact caatagaatt gcctctcaag agcgattctc
     2701 gtatcctcaa ccccttttgc cagcccatgg ccgttacgcg taagaccccc gacgagatag
     2761 cgaagatgcg ggtcgccgga cgtctggctt ccgaggtact ggatttcatc gcgccacacg
     2821 tacgccacgg ggtgtcgacg ggcaaactcg atgccctgtg ccatgactac atggtcaagg
     2881 tccaagacac cgtccccgcg ccgctgaact acgcgccacc cgggtacaag ccctacccca
     2941 agtcgatctg cacttcggtg aaccatcagg tctgccacgg cattccgggc gaccgtgtgc
     3001 tcaagagcgg cgatatcctg aacatcgacg tgacggtcat taaggatgga ttccacggcg
     3061 actcgagccg catgttctac gtcggggagc ctggcgtcca ggcgcgtcgg ctggtcgaag
     3121 tcacatacga atgcatgtgg ctcgggatca tgcaggtgcg cccgggcggc cgcctcggcg
     3181 acgttggcgc ggccatccag gcaaacgctg agcagaacgg cttctcggtc gtgcgagagt
     3241 tctgtggtca cggcatcggg cgcaattttc acgaggatcc acaggttctg cattacggca
     3301 agccgggcac cgggctggta ctcgagccgg gaatgacctt tacggtcgaa ccaatgatca
     3361 acgccggaaa ggcggcgatc cgcgagctgg ccgacggctg gacgatcgtg accaaagatc
     3421 acagcctctc cgctcagtgg gaacacaccg tactcgtcac cgagtcggga taccaggtac
     3481 tgacggtgtc gtcgaactct ccgccgcccc ccgccttcgc ggcgtagacg atggctgcaa
     3541 cgcagcccgc ccgacccggc acgaccgtct cgcagattcg cgcgcaactg caggctgcgc
     3601 gcggcgccct gcgcgaagcc tacctgcggc gagcgcggcc gcacgccctt ctgcgcggcc
     3661 actccaacct cgtcgatcgc atcatcaagg cgatatggcg cgaatcgcag cctgctgcag
     3721 gcgctgcgct gctggccacc ggcggatatg gccgcgcgga gctgttcccg ttctccgata
     3781 tcgacgttct gatcctgttg gcaagggaac cgtcgtccga tgagcgttct cggctcgagt
     3841 ttctcgtcgg gcagctatgg gatgtcggac tggaggtcgg tcacagcgtt cgcaccattg
     3901 cggcctgctt ggcggcggcg gaagccgaca tcacggtgcg cacaaccctg cttgaagcac
     3961 gcttcctcgc aggtagccga acgctgcatc gcaagctcga ggcggccctt caccaggcac
     4021 tcgacccggc ctcgttctat aaggcgaaga agctcgagca ggaacagcgc catgccaagc
     4081 accaggacac tccgtattcc ctcgagccga atctgaagga ggcgcctggc gggctgcgcg
     4141 acctgcatgt cattcagtgg atcgccaggg cagcgggtat cggaagcggc tggcgcgacc
     4201 tcgagaccag cggtctgatc cagcacgacg agatgcgcca gctgtctcgc agcgaatccg
     4261 tgctgcagaa tttgcgaatt cgcttgcact atctcgcggg acgacgggaa gaccggctcg
     4321 tgttcgatct tcaaaacgcg ctcgccgatc agctcggcga ttcggaaacg ccgacgcgcc
     4381 gcgccagcga agtgatgatg cagcgctact accgtacggc gaaaacgata acccagctga
     4441 atacaatcct gatgcagaac ttgggtgccc gccttttccc aaagtccgag gtggaattgc
     4501 agccgctgaa cgaacgcttc cgtgcccgcg gcgaattgct cgaagcctgc gacgaagaat
     4561 tgttcgagcg cgagccgctc gcagtgctcg aaagcttcct cctaatgcag cagcatcacg
     4621 agctccaagg catgactgcg cccacgctgc gggcgctttg gcgcgggcgg cgtcgcatag
     4681 attcccggtt ccggcgcgat ccggttgccc ggctgatgtt tctacaggt
//
LOCUS       DASVDU010000231         8743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_136188, whole genome shotgun sequence.
ACCESSION   DASVDU010000231 DASVDU010000000
VERSION     DASVDU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136188"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..882)
                     /locus_tag="VF226_14855"
     CDS             complement(25..882)
                     /locus_tag="VF226_14855"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075317.1"
                     /translation="MSRLLSGWTLVRIVTLLVYIFLFVPIAVVVLLSFAESRFGSFPI
                     EHFSLRWFVELAHNEAIVRAFQTSMLLGALTSVIATAIGIPAALAIVRYDFPGKNLVT
                     TALIAPILVPEVVLGVALLLFLRWLAMPRSFALLMMGHVIITLPFVLLVVQARLAGIR
                     PEYEEAARSLGANRLQTFLEVTLPLLMPAVLAGMLFAFTISFDDVTATLFWKSAGTET
                     VPTQIFAMLRNSISPEINALGTVMIVITVALPLAAGMVARRFARSGAERTAPAARAEQ
                     YGWESTKEA"
     gene            complement(893..1792)
                     /locus_tag="VF226_14860"
     CDS             complement(893..1792)
                     /locus_tag="VF226_14860"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075318.1"
                     /translation="MADVTIDAGSADTTSAGNPRPWLMMTPALSSVAFLLVAPTCFIL
                     VYSFWLRSATGADQAGFYLDNWREVLTDPFYRAILLQTFRIALITTAVCALVGYFPAF
                     FLARTKVRDKTLLILLLMLPFWISYIIRTLSWINILGASGAVNSALLGLGLIDEPVQF
                     LYNEQSVILGLAHYLLPFMILNIYVSLEGIDRNLEAAARTLGCTPWQAFREVTLPMSL
                     PGLAAGSLLCFVLGAGSYITPLILGGPRQAMFANLIYEAVIIQLDWPLGSALSIALLV
                     MLGLLVAIYNRYVGLKQLAKSFR"
     gene            1925..2974
                     /locus_tag="VF226_14865"
     CDS             1925..2974
                     /locus_tag="VF226_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7075319.1"
                     /translation="MPTDDLPTDDLTKATLATTPAATSAPTSATGTLERITRELDWNL
                     LRTFMVIVQEGGITRAANRLLLRQPTVSNALKRLERQLGHRLILRGPARFQLTEHGEL
                     LYRESVEIYGNISRLAVVLREVKEEISGHVAITMASHVTTPIFDEVLAAFRDAHPKVT
                     YTIDIATSRDVVQAVLQKTSTLGICLVHRHNPRLRYQHIYREHFGFFCGPRHRFFGRA
                     DLTMRDLRGEPLVSFKTDRTTDALRPVALLRAAENFEDRIVGTSSHLEEVRRMIVAGL
                     GIGPLPIHVVDRDVRDGLLWRLPPFENPPAIDVFLVTNPLVHLSRAEQHFVDTLTARI
                     EATPFGERVFPSEFA"
     gene            complement(3011..3661)
                     /locus_tag="VF226_14870"
     CDS             complement(3011..3661)
                     /locus_tag="VF226_14870"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEX7075320.1"
                     /translation="MVETLSREVAIEPAQAALLVVDVQNFCGRRDGGEYKTLDPETLD
                     ARYGYYFRQLETVALPSMQRLIAACRQRRIEVLYTVIESLTRDGRDRSLDYKITGFHV
                     PRGSWDGKVLDEIAPAEDEIVLPKTSSSVFISTNIDYLLRNLGIRQLMICGVCTDQCV
                     ESAVRDACDLGYLVTLATDACATHSQERHDNTLRAIKGYCRQRTTDQLIEELTPVD"
     gene            3794..4585
                     /locus_tag="VF226_14875"
     CDS             3794..4585
                     /locus_tag="VF226_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075321.1"
                     /translation="MAQADSLLEVRDLSKHFGGVVALDRLSLSIAPNRITGLIGPNGS
                     GKTTAFNLLTGVLKPSGGRITFRGRDITGAPPHRNAALGLARTFQNIRLFKDLPVIDN
                     IAVGLHMRHGSGLWPTLLSWPSAWRSERLIRARAHELLALLGMADRAEVPVGILPYGD
                     QRRVELGRALATEPRLLLLDEPSAGMNAVETMELMRTVRHIQENFDLSVLLVEHDMKM
                     VMGLCEWIYVINQGQLLAAGPPEEIQNHPEVVEAYLGHNQSRAHA"
     gene            4578..5303
                     /locus_tag="VF226_14880"
     CDS             4578..5303
                     /locus_tag="VF226_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075322.1"
                     /translation="MLEVRDIDVYRGETHVLHGLELGVRPGEIVALIGANGAGKTTAL
                     RTISGLLRSRRGSIGFRPDPERPWTDITRLPAEEIVGLGISHCPEGRQIFGNLTVREN
                     LMLGAFLRQDAASVRRDAGELCRLFPVLAERAGQLGTRLSGGEQMMLAIARALMSRPK
                     LLLLDEPSLGLAPRLIEQVFETLERIRAEGTTVLLVEQNAAQALAIADRAYVMESGSI
                     TLSGTGAELAGNEQVRRAYMGAA"
     gene            5319..6212
                     /locus_tag="VF226_14885"
     CDS             5319..6212
                     /locus_tag="VF226_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075323.1"
                     /translation="MSSYIATQIVNGIILGSMYALVAIGFSMIYGIVRLINFAHGDIV
                     MIGAFATLAFGALLGLPFPLVVVLVVLAGAVVGLLVERLAFRPMRGAPQVTGFIASLA
                     VSILIENLGLMTVTAQPRNFLVPDYLQHIVHLGGLTLRVVDLAIVAVSILLMALLVLF
                     VRYTKLGIAMRATAENLDVARLMGIRINRTIALAFAIGSGLAAAGGMMWGSKYGQIDP
                     LMGFVPGLKSFVAAVIGGVGSVPGAVLGGYLLGLGEVLFVGLLPPEYSGFRNAFVFGT
                     LILILLVLPNGLLGRSEEERA"
     gene            6209..7255
                     /locus_tag="VF226_14890"
     CDS             6209..7255
                     /locus_tag="VF226_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075324.1"
                     /translation="MIPLAGSLRLPLLAALGALFVWLLESLLDPYALSVAMFVGINII
                     LAVSLNITNGFTGLFSLGHPGFMAVGGYVTALLTMLPARKGLMLPDLPSWLAGLHWGF
                     LPALLAGGLCAVLVAVVVGFPVLRLRGHYLAVATLGFIIIVQTLLINLVGLTRGARGL
                     NGLSPLTGPWWVYGWVVVTIYTCWTIKHASLGRAMLAIRENEMAAHCLGVNVTRVRLL
                     AFVLGAFFAGIAGGLWAHLVTLVTPTSFSIVMSFMLVAMVVIGGSGSITGAVIAAAAL
                     PLASEAVRPIEEGLGLYGLSQVLVALGVLLILIYRPQGLFGTSEPRFLESRPAALAHP
                     REKPDTLTEKGGIR"
     gene            7252..8412
                     /locus_tag="VF226_14895"
     CDS             7252..8412
                     /locus_tag="VF226_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075325.1"
                     /translation="MRIPFLGTLAAGLVASTFALGVQAAEPIKIVALYNLTGGMSSLD
                     QPSLNGAKLQAKMINDAGGLLGGRMIEVVPIDTKTDQKETATAAKRAVGMEDVTAGIG
                     YSDTTFVLAAAPLFQDAGIPFVTSGATSPLLPEMVGDMLFLVPFGDNIQAYAMAEYVY
                     NTMGIHDVVVWTDNGMDYTTGLSKYFQERFEKLGGNIVLNDVFLTGDRDFSAQVARLQ
                     ANQDKAGAVFVAAGPDEAGIIVKQIREAGIDMPILGGDGFDTPLVGSVPGEDLANDVY
                     FTTHAYADLESEAIKTFQQGYKEAYGTDPENAFAALGYDAVGLLADAIKRAGSTDHEK
                     LRDALASTDGYEGVTGTISYENGSRVPLKTVAVMKVENGDFSLVTTMTPSAE"
     gene            8496..>8743
                     /locus_tag="VF226_14900"
     CDS             8496..>8743
                     /locus_tag="VF226_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX7075326.1"
                     /translation="MTDQQMTLLAEDEPPPVGIERPEGPSPIFMSCDHAGKRIPRALG
                     TLGLDEPDRSRHIAWDIGALAVAQGLSARLDASLIHQR"
BASE COUNT         1475 a         2983 c         2806 g         1479 t
ORIGIN      
        1 caagccgttt cgtcatatcc atggctaagc ctccttggtt gattcccatc cgtattgttc
       61 cgcccgggcc gccggcgccg tgcgttcggc cccgctccgg gcaaagcgcc gcgccaccat
      121 gcccgccgcc aaaggcagcg cgacagtgat cacgatcatg accgtgccca gcgcgttgat
      181 ctccggactg atcgaattcc tcagcatggc gaagatctgg gtcggcaccg tctcggtgcc
      241 ggcggacttc cagaacagcg tcgcggtgac gtcgtcgaag ctgatggtga aggcgaacag
      301 catgccagcg agcacggcgg gcatcagcaa cggcagagtc acctcgagaa aggtctgaag
      361 acggttggcg ccgagcgacc gcgccgcctc ctcgtattcg ggccggatcc ccgcgagccg
      421 ggcctggacc accagcagca cgaagggaag cgtgatgatg acgtgcccca tcatcaggag
      481 cgcgaagctc cgcggcatgg cgagccagcg caggaacagc agaagcgcga cgcccagcac
      541 cacctccggc accaagatcg gcgcgatcag cgccgtggtg acgaggttct tgccgggaaa
      601 gtcgtagcgc acgatcgcca gggccgccgg aatgccgatc gccgtggcga tcaccgaggt
      661 cagggcgccc agcagcatcg aggtctggaa ggcccgaacg atcgcctcgt tatgcgcgag
      721 ctccacgaac cagcgcaggc tgaaatgctc gatcgggaag gagccgaagc ggctctcggc
      781 gaaggacagc agaacgacca ccgcgatcgg cacgaacagg aagatgtaga ccagcagcgt
      841 gacgatgcgg accagggtcc agccggacag cagcctgctc atcccgcgcc cctcaccgga
      901 agctcttggc gagctgcttc agcccgacat agcggttgta gatcgcaacc agcaggccga
      961 gcatgaccag aagcgcgatg gacaaggccg agcccagcgg ccagtcgagc tgtatgatca
     1021 ccgcctcgta gatcaggttg gcgaacatcg cctggcgcgg cccgcccagg atcagcggcg
     1081 tgatgtagct ccccgcgccc agcacgaagc acagcagcga cccggccgcc agccccggca
     1141 acgacatcgg cagcgttacc tcgcgaaagg cctgccacgg cgtgcagccc agcgtgcgcg
     1201 ccgccgcctc gaggtttcga tcgatgccct ccaggctgac atagatgttg aggatcatga
     1261 agggcagcag gtagtgggcc agccccagga tcaccgactg ctcgttgtag aggaactgca
     1321 cgggctcatc gatgagcccg aggccgagaa gggccgagtt caccgctccc gacgctccca
     1381 ggatgttgat ccaggacagc gtccggatga tgtagctgat ccagaacggc agcatcagca
     1441 acaggatcag cagggtcttg tcgcggacct tcgtgcgggc gaggaaaaag gccggaaaat
     1501 agccgacaag ggcgcagacc gccgtggtga tcagcgcgat cctgaaggtc tgcagcagga
     1561 tcgcccggta gaacgggtcg gtcagcacct cccgccagtt gtcgagatag aagccggcct
     1621 gatcggcgcc cgtcgcgctg cgcagccaga acgaatagac gaggatgaag caggtcggcg
     1681 cgaccagcag gaatgccacc gagctcagcg ccggcgtcat catcagccat ggccgaggat
     1741 taccggccga tgtcgtgtcc gccgatcccg cgtcgatcgt cacgtccgcc attcatctgc
     1801 cgccctgctg cgccgccggc gcgatggacg ctcccttttg accgattagg gcgcagggct
     1861 ggacattgcg caaatagata tgaagaatat tgggtatagc ctcgtattat gattggttcg
     1921 ttgcgtgccg accgatgacc tgccaaccga cgacctgacg aaagcgacac tcgccacgac
     1981 gccggccgcg acatcggccc cgacatcagc cacgggcacg ctggagcgga tcacccgcga
     2041 gctggactgg aacctgctgc ggaccttcat ggtgatcgtg caggaggggg gaatcacccg
     2101 cgcggccaac cggctgctgc tgcgccagcc gacggtgagc aacgccttga agcggctgga
     2161 gcggcaactc ggccatcggc tgatcctccg cggcccggcc cggttccagc tcaccgagca
     2221 tggcgagctg ctgtaccggg aatccgtcga gatctacggc aatatctcgc gtttggccgt
     2281 cgtgctgcgc gaggtgaagg aggagatttc aggacatgtg gccatcacga tggccagtca
     2341 tgtgaccacg cccatcttcg acgaggttct ggcggccttt cgcgatgccc acccgaaagt
     2401 cacctatacc atcgacatcg caaccagcag ggatgtcgtg caggcggtgc tccagaagac
     2461 cagcacgctc ggcatctgcc tcgtccaccg gcacaatccg cggctccgct accagcacat
     2521 ctatcgcgag catttcggtt tcttctgcgg cccacgccat cggttcttcg gccgcgccga
     2581 cttgacgatg cgggacctgc gcggcgagcc gctcgtctcc ttcaagacgg accgcaccac
     2641 ggacgcgctg cgtccggtcg cgctcctgcg cgccgcggaa aatttcgagg atcggatcgt
     2701 cggcacatcg agccatctgg aggaggtcag acggatgatc gtcgccggcc tcgggatcgg
     2761 gccgctgccg atccatgtcg tcgaccgcga cgtccgcgac gggctgctgt ggcgcctgcc
     2821 gccgttcgag aacccgccgg cgatcgacgt cttcctggtc accaatcccc tggtccatct
     2881 gagccgggcc gagcagcact tcgtcgacac cctgacggca cgcatcgagg cgaccccgtt
     2941 cggcgagcgc gtcttcccat ccgagtttgc ctgaagggtc cagcgcgccc gcttcgatca
     3001 atcggctcga tcaatcgact ggggtcagtt cctcgatcag ctgatcggtg gttcgctgcc
     3061 ggcaatagcc cttgatcgcc cgcagcgtgt tgtcgtgccg ctcctggcta tgggtggcgc
     3121 aggcgtcggt cgccagcgtc accaaatagc cgaggtcgca ggcgtcgcgc accgccgatt
     3181 ccacgcactg gtcggtgcag acgccgcaga tcatgagctg ccggataccg agattccgca
     3241 gcaggtagtc gatattggtg gagatgaaga cgctggagga ggtcttcggc agcacgatct
     3301 cgtcctcggc gggggcgatc tcgtcgagca ccttcccgtc ccaggagccg cgcgggacat
     3361 gaaagccggt gatcttgtag tccaggctgc ggtcccggcc gtccctggtc aggctttcga
     3421 tcaccgtgta cagcacctcg atccgccgtt gccggcaggc cgcgatcagg cgctgcatgc
     3481 tgggcagcgc caccgtctcg agctgccgga aatagtagcc gtagcgcgca tccagcgttt
     3541 cggggtcgag cgtcttgtac tcgcccccgt cgcgccggcc gcagaaattc tgcacgtcca
     3601 caaccagaag cgccgcctgc gccggctcga tcgccacctc gcgactcagt gtttccacca
     3661 ttctcctctc ccgctctcgg gctccgcgga gccggcgcaa gcttgtaata tttggaacgg
     3721 tctgtcatcc tccgtttcaa aaactacggc ccacaaaggg gtcggcaggc gcagggagac
     3781 attcgaccga tccatggccc aggcggattc attgctggag gtgcgagacc tctccaagca
     3841 cttcgggggc gtggtggcgc tcgaccggct gagcctgtcg atcgccccga acaggatcac
     3901 cggcctcatc ggcccgaacg gctccggtaa gaccaccgcc ttcaacctcc tgaccggcgt
     3961 cctcaagccg agcggtgggc gcatcacctt ccgcggtcga gacatcaccg gcgcgccgcc
     4021 gcaccgcaac gccgccctcg gcctcgcccg gacctttcag aacatccgcc tgttcaaaga
     4081 cctgccggtg atcgacaaca tcgcggtcgg cctgcatatg cgccacggta gcggcctctg
     4141 gccgacgctc ctgtcctggc cgtcggcgtg gcgatcggag cgactgatcc gcgcgcgcgc
     4201 ccatgagctc ctcgcgctgc tcggcatggc ggaccgggcg gaggtgccgg tgggaatcct
     4261 gccctatggc gaccagcgcc gggtcgaact cggccgcgcg ctggccaccg agcccaggct
     4321 tctcctgctc gacgagccct cggccggcat gaacgcggtc gagaccatgg agctgatgcg
     4381 taccgttcgg catatccaag agaacttcga cctgagcgtg ctgctggtcg agcacgacat
     4441 gaagatggtt atgggcctgt gcgaatggat ctacgtcatc aaccagggcc agctcctggc
     4501 cgccgggccg ccggaggaga tccagaacca tcccgaggtg gtcgaggcct atctcggcca
     4561 caatcagtcg cgagcccatg cttgaagtcc gggacatcga cgtctatcgc ggcgagacgc
     4621 atgtcctgca cggcctcgag ctgggcgtgc ggccgggcga gatcgtcgcc ctgatcgggg
     4681 ccaacggcgc cggcaagacc acggccctgc ggacgatctc cggcctcctg cgaagccgtc
     4741 gcggatcgat cggcttccgg cccgacccgg agcggccctg gaccgacatc acgcggctgc
     4801 cggcggagga gatcgtgggg ctcggcatct cccattgccc ggagggacgg cagatcttcg
     4861 gcaatctgac agtgcgcgag aacctgatgc tgggggcgtt tctgcgccaa gacgccgcga
     4921 gcgtgcgccg agacgcgggt gagctgtgcc gcctgttccc cgtgctcgcc gagcgcgcag
     4981 gtcagctcgg cacgcggctg tcaggcggcg agcagatgat gctcgccatc gcccgcgccc
     5041 tgatgagtcg gccgaagctt ctgctgctcg acgagccgtc gctcggcctg gccccgcggc
     5101 tgatcgagca ggtcttcgag acgctggagc ggatccgcgc cgaaggcacg accgtccttc
     5161 tggtcgagca gaacgccgcc caggcgctgg caatcgccga tcgtgcctat gtcatggaat
     5221 ccggctccat cacgctcagc ggcaccggtg cggagcttgc cggcaacgag caggtccgca
     5281 gggcctatat gggagccgcc tgatgggagc cgcatgagat gagcagctac atcgccacgc
     5341 agatcgtcaa cggcatcatc ctgggcagca tgtacgcgct cgtggcgatc ggcttctcga
     5401 tgatctacgg catcgtccgg ctcatcaact tcgcgcatgg cgacatcgtg atgatcggcg
     5461 ccttcgccac gctggcattc ggcgccctcc tcggcctgcc ctttcccctg gtggtcgtgc
     5521 tggtggtact cgcgggtgcg gtcgtcggcc ttctggtcga acggctcgcc ttccggccga
     5581 tgcgcggcgc gccgcaggtg accggcttca tcgcctcgct cgccgtctcg atcctgatcg
     5641 agaatctcgg tctgatgacc gtgacggccc agccgcggaa cttcctggtg ccggactacc
     5701 tgcagcatat cgttcatctc ggcggcctca ccttgcgcgt cgtcgacctg gcgatcgtcg
     5761 ccgtctccat cctgctgatg gccctgctcg tgctcttcgt gcgctacacc aagctcggga
     5821 tcgccatgcg ggccacggcg gagaatctcg atgtggcccg gctgatgggt atccggatca
     5881 accggaccat cgccctcgcc ttcgccatcg gcagcgggct ggccgccgcg ggcggcatga
     5941 tgtggggcag caagtacggc cagatcgacc cgctgatggg cttcgtgccc gggctcaaat
     6001 ccttcgtcgc cgcggtgatc ggcggcgtcg gctcggtgcc cggcgccgtg ctcggcggtt
     6061 acctgctggg cctcggcgag gtgcttttcg tcggcctgct gccgcccgaa tattccggct
     6121 tccgcaacgc cttcgtgttc ggaacgctga tcctcatcct gctggtcctg ccgaacggac
     6181 tgctcggccg cagcgaggag gagcgggcat gatccccctc gccggatcgt tgcgcctgcc
     6241 gctgctggcg gcgctcggcg ccctgttcgt ctggctcctg gaatcgctgc tcgatcccta
     6301 tgcgctttcg gtggccatgt tcgtcggaat caacatcatc ctcgcggtga gcctcaacat
     6361 caccaacggc ttcaccggcc tcttctccct cggccacccc ggcttcatgg cagtcggcgg
     6421 ttacgtcacg gccttgctga ccatgctgcc ggcccggaag gggctgatgc ttccggacct
     6481 gccctcctgg ctcgccggcc tgcattgggg cttcctgccg gccctgctgg ccggcggcct
     6541 ctgcgccgtc ctggtcgcgg tcgtcgtcgg ctttccggtg ctgcgcctgc gcggccatta
     6601 tctggcggtc gccacccttg gcttcatcat catcgtccag acattgctga tcaacctggt
     6661 gggcctgacc cgcggggcgc gcgggctgaa cgggctgtcg ccgctcaccg ggccatggtg
     6721 ggtctatggc tgggtcgtcg tcacgatcta cacctgctgg accatcaagc atgcctcgct
     6781 ggggcgggcc atgctggcga tccgcgagaa cgagatggcg gcgcactgcc tcggcgtgaa
     6841 cgtcacgcgc gtccgcctgc tcgccttcgt gctcggtgcg ttcttcgccg ggatcgccgg
     6901 ggggctgtgg gcgcatctcg tgaccctggt gacgccgacc tccttctcca tcgtcatgtc
     6961 attcatgctc gtggccatgg tggtgatcgg cggctccggc agcatcaccg gcgcggtcat
     7021 cgcggccgcc gccctccccc tggcctccga agcggtccgc ccgatcgagg aaggcctcgg
     7081 gctctacgga ttgagccagg ttctggtcgc cctgggcgtc ctgctcatcc tgatctaccg
     7141 gccgcaaggc ctgttcggga ccagcgagcc gcggttcctc gagagccgtc cggcagcgtt
     7201 ggcccaccct cgggagaaac ccgacacctt gacagaaaag ggaggcatcc gatgagaata
     7261 ccgtttctag gcacgctcgc cgcaggcttg gtcgcctcga ccttcgcgct cggcgtccag
     7321 gccgcggagc ccatcaaaat cgtggctctc tacaacctga ccggcggaat gtcctcgctg
     7381 gaccagccct cgctgaacgg cgcgaagctt caggccaaga tgatcaacga cgcgggcggg
     7441 ctcctgggcg gccgcatgat cgaggtcgtg ccgatcgaca ccaagaccga ccagaaggag
     7501 accgcgacgg cggccaagcg cgcagtcggg atggaggatg tcacggctgg catcggctac
     7561 agcgacacca ccttcgttct cgccgctgcc ccgctcttcc aggacgcggg catccccttc
     7621 gtcacctctg gagcgacctc gccgctgctg cccgagatgg tcggcgacat gctgttcctc
     7681 gtgcccttcg gcgacaacat ccaggcctac gcgatggccg agtacgttta caacacgatg
     7741 ggaatccacg acgtggtggt ctggaccgac aacggcatgg actacaccac cggcctctcg
     7801 aaatacttcc aggagcgctt cgagaagctt ggcggcaaca tcgtcctcaa cgacgtgttc
     7861 ctgaccggcg accgcgactt ctcggcccag gtggcgcgcc tccaggcgaa ccaggacaag
     7921 gcgggtgcgg tcttcgtcgc cgccggcccg gacgaagccg gcatcatcgt caagcagatc
     7981 cgcgaggccg gcatcgacat gccgatcctg ggcggcgacg gcttcgatac gccgctggtc
     8041 gggagcgtgc cgggcgaaga cctcgcgaac gacgtctatt tcacgaccca tgcctatgcc
     8101 gatctggaga gcgaggccat caagacgttc cagcaaggct acaaagaggc ctacggcacc
     8161 gatccggaga atgcgttcgc ggcgctcggc tacgatgccg tcgggctcct ggcggacgct
     8221 atcaagcggg cgggctcgac cgatcacgag aagctgcgcg atgcgctggc gtcgaccgac
     8281 ggttacgagg gcgtcaccgg gacgatctcc tacgagaacg gcagccgcgt gccgctgaag
     8341 accgtggcgg tcatgaaggt cgaaaacggg gatttctcgc tcgttacgac catgacgccc
     8401 tccgcggagt gaggcaatcc cgcccggagg gcgcggtcaa tggaccgacc gcgccctccg
     8461 ggcagacccg atccaacctg taccagacaa gcatcatgac cgatcaacaa atgaccctcc
     8521 tggcagagga cgaaccccct cccgtgggga tcgagcgtcc ggaggggccg tcgcccatct
     8581 tcatgagctg cgatcatgcc ggcaaacgca tcccgcgggc gctcggcacg ctcggcctcg
     8641 acgagccgga ccgcagccgc cacattgcct gggacatcgg cgcgctggcc gtggcgcagg
     8701 ggctttcggc ccgcctcgat gcatccctga tccatcagcg cta
//
LOCUS       DASVDU010000232        11288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1568851, whole genome shotgun sequence.
ACCESSION   DASVDU010000232 DASVDU010000000
VERSION     DASVDU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568851"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF226_14905"
     CDS             complement(<1..494)
                     /locus_tag="VF226_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7075327.1"
                     /translation="MASTRIVIVDDHPLFRKALNQALATSLHAAEIVEVGSLDELTGR
                     LAADSNVDLILLDLSMPGVQGLSGLLYLRAEHPEIPVVVVSASEDPATIRGAIGFGAS
                     GYVPKSQPVDRIRTAVRKVLEGEIWVPPEIDIEGQAEETSELVARLSTLTPQQVRVLM
                     MLGEG"
     gene            965..1225
                     /locus_tag="VF226_14910"
     CDS             965..1225
                     /locus_tag="VF226_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEX7075328.1"
                     /translation="MIEREERKPYWRHTKWQMLASLLPFLLVVIVFPLYADKLNSNKF
                     LGFPLGYFLTGHGLILIAVVTVASFVNRQDAIDHWHGGHENV"
     gene            1284..3122
                     /locus_tag="VF226_14915"
     CDS             1284..3122
                     /locus_tag="VF226_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX7075329.1"
                     /translation="MPLSSHARLANPRLGIYFGIITSVFVALVLVLLIFEQLGASDTL
                     LRFLMLGGPLALYAAIGLSAGCNEPLDFFASGRRVPPFFNGLVLAVSAMGATGLVAGT
                     GLLFLHGFDGWFLVIALTGGFVVMAILIAPYLRKFGAYTVPSYLGRRCENRLVRVAAA
                     GFAAIPMLLVIAAEIRMGIFAGSWLTGQSQQMMAVILASAIILAIVLGGMRSASWANS
                     SESIAVLLALIVPTTMVAVAVTYLPFAQLSHGPVLRALGRLESHQGMPIPILSPFAFD
                     IAGLELESIVQRMSKPFGSIGSASFVLACLTLMSGVAAAPWLLPRVGTTPSVYATRKS
                     LGWATFIVGMILLTASSVAVFMRDVVMDTLVGYSQSQLPEWFRSLEAAGYAGAYGQVP
                     RLPISSFSFKRDAVLFALPIAYGYPSVIVYLALAGAFAAAVAGASLSSLALGTTLSED
                     LVNGMRWEPAPSRIRLTLARVSIAFAAIVGTGLALITPADPLKLFLWALALSASGLFP
                     VLLLSIWWKRLGAFGALAGMSAGFGVAILVMIAGETGLIGLNSVLAGVAGMPAGFLAA
                     IGASWVSGMPSRNVLELVHDMRVPGGETVHDREMRLLRLKQRTQRL"
     gene            3170..4159
                     /gene="pip"
                     /locus_tag="VF226_14920"
     CDS             3170..4159
                     /gene="pip"
                     /locus_tag="VF226_14920"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEX7075330.1"
                     /translation="MRFHDLPTNHTDASTRHNLYPPIEPYAQGRLAVSGGHEIYYELC
                     GNPHGKPALLVHGGPGGGSNATMRRYHDPSRYHIILFDQRGCGRSTPHASLEHNTTWD
                     LVEDMERLRRHLGVDQWQLLGGSWGSTLSLVYAETHPERVSELILRGIFLLRKAEIDW
                     FYQEGCSWIYPDAFEAYQSVIPPEERSDMVGAYYKRLTHRDRQVQLEAAKAWSIWEAT
                     TLSLHRDAERIRNFGSDNYAIAFARIECHYFINRGFLASDGQLIADAHRLSGIPGVIV
                     HGRYDVVTPLKNAWDLKKAWPRADLRIVPDSGHAMTEPGIIHELVSATRRFSP"
     gene            4239..4328
                     /locus_tag="VF226_14925"
     tRNA            4239..4328
                     /locus_tag="VF226_14925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4273..4275,aa:Ser,seq:gga)
     gene            4822..6147
                     /locus_tag="VF226_14930"
     CDS             4822..6147
                     /locus_tag="VF226_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7075331.1"
                     /translation="MKRLLAVLSAFAFAVGVSPAEAQSPTGKVTVSTSYSKDVTDPVK
                     KAFEKAHPGVTLDVQNRNTNAGVKYLEETRGNNQTDLFWASAPDAFEVLKGKGLLQKY
                     EPKATGIPEKIGAFPINDPDGYYFGFAASGYGIMWNERYAKANNLPDPKEWSDLAKAV
                     YFDHVAMAAPSRSGTTHLTIETILQGDGWEKGWRTIKEMAGNFRQITERSFGVPDAVN
                     SGEVGFGIVIDFFAFSAQASGFPIKFVYPTVTTIVPANVAIVANAPNKAGAEAFIEFL
                     LSPEGQEVLLEPSIRRLPINPAVYAKAPADYPNPFKDPSLGARVKFDVNISEKRTAVV
                     DALFDQMITFQLDNIKSATKAVHEAEAALAKKDNAEARALVKEARDLIAAMPISEEQA
                     SSPEIAGAFTGGKQKGARQAELEQQWAAFAREHYAQAQAKAEQALKLAR"
     gene            6207..7913
                     /locus_tag="VF226_14935"
     CDS             6207..7913
                     /locus_tag="VF226_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7075332.1"
                     /translation="MALVLPRREGFLSVWPGQLLVAALIAVILLLFLIIPVATVIYVA
                     FTEKGTGAFTLVNFVDFLHTDLFVRSFWNSIYVSAMAVVLASLFALPLATLTTRFEFR
                     GALIVQTLGFIPLIMPPFVGAVAMQLLFGRNGTVNLLLDDWLGFKIPFMEGLNGVIFV
                     QAVHYFPFILVNVSAALRNIDRSMEEAAQNLGCSGFRLFRRVVFPLALPGYLAGASLV
                     FVKVFDDLATPLLLNVKDMLAPQAYLRVTAIGIADPMGYVISVVLIIVSIVAMWASAA
                     AMRGRDYATVQRGGGGLAKRKMGLWGNVAAYAVVILILLLVLAPHIGLLLLSFATIWS
                     FSPLPDGFTLAHYGRVFGESFIYVKNTLLYASLAGLIDVVLGVAIAYIVLRTRLFGRE
                     WLDWAASAALAVPGVVLGIGYLRTFYGVQLPDGRPLATLWIMIVIALAIRRLPYALRA
                     CYAALQQISASLEEAAENLGATKWRTVRRIVVPLMMGGILAGFISSFATAAVELSATL
                     MLVQSDTDAPIAYGLYIFMQSAAGRGPGAALGVIAVALVALCTILSHIVIERGQRATS
                     RR"
     gene            7943..9034
                     /locus_tag="VF226_14940"
     CDS             7943..9034
                     /locus_tag="VF226_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075333.1"
                     /translation="MNRAAWNHDSAIAPVGVRIESVDVSYGSNHVLKSVSLDIAPGEF
                     FAFLGPSGCGKTTLLRLIAGFNQADKGRVLVGGEDISRLPPWKRDVGMVFQSYALWPH
                     MTVWRNVAFGLEERRLPRQEIEKRVAEALELVGLAGLADRRPSQLSGGQQQRVALART
                     IAVRPKVLLLDEPLSNLDAKLRMQVRRELRDLQQRLGLTTIFVTHDQEEANTICDRIA
                     VMNDGTVQQVGTPMELYERPANLFVATFLGTANVLEGELVGSGPERTYVIGEGIALPV
                     PPEANVSPGAKLVFRPQECRIGASGEGMLPGTVAYREFLGSTVRYGVRVGATEIAVDT
                     PFQAGDGLHEIGDAVGVTLPLGAALWLAS"
     gene            9485..10480
                     /locus_tag="VF226_14945"
     CDS             9485..10480
                     /locus_tag="VF226_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075334.1"
                     /translation="MLMPAELLEVEDLTIRFHPGGPAVVDRLSISLTQGETLSLVGES
                     GCGKSLTALAIMGLLPPGARTTGRVRLEGEDLLAAGERRLADLRGNRLAMIFQEPMTS
                     LNPAFRIGEQIAESVVRHRGLQRSKARERALEMLRAVRIPAPEKRLDAYPHQLSGGMR
                     QRVMIAMALVNAPNILIADEPTTALDVTIQAQILALIRELQREAGMAMILVTHDLGVV
                     AETADRVAVMYAGRIVETAAAPDLFADPQHPYTLGLIGSLPSLGRRQGRLATIPGSVP
                     PPDRFPPGCRFATRCPFADRRCYAEVPRLRAIGAEGHKVACFKAPIEHLGVEQAA"
     gene            10477..>11288
                     /locus_tag="VF226_14950"
     CDS             10477..>11288
                     /locus_tag="VF226_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7075335.1"
                     /translation="MSSPGAGETILEAREVVRYFGGQRTFPFGRAPLIRAVDGVSLAI
                     RRGETFAIVGESGCGKSTLARVLLNLIPPTSGSVVFEGREITSAPAEEMRRLRRNMQL
                     IFQDPFASLNPRMTVGALIGEPLEVHGIAKGAERRRRVAKMLRLVGLSPDYANRYPHE
                     FSGGQRQRIGIARALATEPRLVIGDEPVSALDVSIRAQVINLLEALKEELALTLVLVA
                     HDLAVIRHTSDRVAVMYLGEIVEIADCDALFEAPRHPYTRSLIEAIPVPVPGA"
BASE COUNT         1843 a         3700 c         3576 g         2169 t
ORIGIN      
        1 ccctccccca gcatcatgag cacgcggacc tgctgcggcg tcagcgtcga caggcgcgcg
       61 acgagctcac tggtttcttc cgcctgcccc tcgatgtcga tctccggcgg cacccagatt
      121 tccccttcca gcaccttgcg cacggccgtc cgaatgcgat ccacaggttg cgatttcggg
      181 acgtagcccg aggcgccaaa cccgatcgcc ccgcggatgg tggccggatc ctcgctggcg
      241 gaaacaacga caacgggaat ctccgggtgt tcggcacgca aatagagaag ccccgatagg
      301 ccctggacac ctggcatcga cagatcgagc aggatgagat ccacattgct gtcggcggca
      361 agtctcccgg tcaattcatc gagagagccg acttcgacga tctcggcggc atgaagggac
      421 gtggccaaag cctgattgag ggccttgcgg aacagcgggt gatcgtcgac gatcacgatg
      481 cgggtcgaag ccataattca gtccttcccg gggaaggggc gtgcctgccc aaacggcacg
      541 tcggccctcg cccggcaaca gctggacacc atgaaatcgc gccggtcacg atttaagcaa
      601 gccgccgaac gattatgcaa gcttatcctg cctgctcacc tgagctggcc aggccgggcc
      661 gagcctgacc gaaacagatt tgcgctggcc cgcccaccgc ggggacggcg gcgttctggg
      721 cgtagcgccc tgattcccac gttctcttgg cgcctacggt tgggccgggg ggcgaactcg
      781 gaatcaaaaa gacgcaagtt caaggacctg ctagcgtgag gcagatcgcg aggttcgctc
      841 acctctttcg ccaaatgtgg cgaatttctc gatcatcaca ctagtgtccc gattccgaag
      901 ttcgctgtgc gcgcgtgagt tcgagagcga acttcggaaa cctcagccat cgggggattt
      961 tttgatgatt gagcgtgagg aacgcaagcc gtattggcgg catacgaaat ggcagatgct
     1021 ggcgagcctg ctgcccttcc ttctggtcgt catcgtcttt ccgctctatg cggacaagct
     1081 caactcgaac aagtttctgg gcttcccgct cggctacttt ctgaccggac atgggctcat
     1141 cctgatcgcc gtcgtcaccg tggcgtcctt cgtcaacagg caggacgcca tcgaccactg
     1201 gcacggaggc cacgagaacg tctgatcggc tgcggaccgc gacccgccct ccgcctgttg
     1261 aatcctgacg gaacctcctg cccatgcccc tctcatcgca cgcccgcctc gccaatcccc
     1321 gcctcgggat ctacttcggg atcatcacca gcgtatttgt ggcgctggtc ctcgtgctgt
     1381 tgatcttcga gcagctgggt gcatcggaca ccttgctgcg ctttctcatg ctcggcggac
     1441 cgctggcgct ctacgccgca atcgggcttt ccgcaggttg caacgagcca ctcgacttct
     1501 ttgccagtgg acgccgtgtg ccgccgttct tcaacgggct cgtgctggcc gtgtcagcca
     1561 tgggtgcgac cgggctcgtt gccggtaccg gcctcctgtt cctccacggc ttcgacggct
     1621 ggttcctcgt gatcgcgctg acgggcggct tcgtcgtcat ggcgatcctg attgctccct
     1681 acttgcggaa attcggcgca tacaccgtcc cgagctatct cggccgcaga tgcgaaaacc
     1741 gcctcgtgcg ggtcgcggct gccggattcg cagccatccc gatgctgctg gtcatcgcag
     1801 cggagatccg gatgggcatc ttcgcaggat cgtggctgac cggacaatcc cagcagatga
     1861 tggccgtcat tctggcgtct gcgattatcc tggccatcgt tctcggcggc atgcggtcgg
     1921 cgagctgggc gaacagctcg gaaagcatcg ctgttctgct ggctctgatc gttcccacga
     1981 ccatggtggc cgtcgcggtg acttacctgc cgttcgcgca gctcagccac ggaccggtgc
     2041 tgagggccct cgggcgcctc gaatcccatc agggcatgcc gatcccgatt ctctcgccgt
     2101 tcgccttcga tatcgccggc ctggagctcg aatccatcgt gcagcgcatg tcgaagccgt
     2161 tcggcagcat cggttcggcc agcttcgtgc tggcgtgcct tacactgatg tccggcgtcg
     2221 ccgcagcgcc ctggctgctc ccgcgcgtcg gcacgacgcc cagcgtctat gccacgcgca
     2281 agtcgctcgg ctgggcgacg ttcatcgtcg gcatgatttt gctgacagcc tcctcggtcg
     2341 ccgtgttcat gcgcgacgtc gtcatggaca cgctggtggg ctacagccag agccagctgc
     2401 cggaatggtt ccgctcgctc gaggccgccg gctacgcagg ggcttacgga caggtgccac
     2461 ggctgccgat cagcagcttc agcttcaagc gtgatgccgt gctgttcgct ctgccgatcg
     2521 cctacggcta cccgtccgtg atcgtttatc tggcactcgc gggcgccttc gcggctgccg
     2581 ttgccggcgc cagcctgtcc tcgctggctc tgggcacgac gctctcggaa gacctcgtca
     2641 atgggatgcg gtgggagcct gctccaagcc gcatccggct caccctggcg cgcgtatcga
     2701 ttgcctttgc cgccatcgtg ggcacgggcc tggcgctgat cactcccgca gatcctctga
     2761 agttgtttct gtgggcgctg gccttgtcgg cctcgggtct gtttccggtg cttctcctgt
     2821 cgatctggtg gaagcggctc ggcgcattcg gcgctctggc cggcatgagc gccggattcg
     2881 gagtcgccat cctggtgatg atcgcgggcg agaccggctt gatcggactt aacagcgtcc
     2941 tggccggagt tgccggcatg ccggcgggat ttcttgctgc catcggcgca agctgggtga
     3001 gcggcatgcc gtcacgcaac gtcctcgagc tcgttcacga tatgcgtgtt cccggcggcg
     3061 agacggtgca tgatcgtgag atgcgtctgt tgcgtttgaa gcaaaggacg caacgcctgt
     3121 agctgccagg gcccgatcga gttcagcccc ccggaggacc acggtttcaa tgaggtttca
     3181 cgatttgccc accaaccaca cagatgcgtc cacgcggcac aacctctatc cgccgatcga
     3241 gccctatgcc cagggccgtc ttgctgtctc gggtgggcat gaaatctatt acgagctgtg
     3301 tggaaatccg cacgggaagc ctgctcttct cgtgcacggc ggccccggcg gcggcagcaa
     3361 cgccaccatg cgccgctacc acgacccgtc gcgctaccac atcatcctgt ttgatcagcg
     3421 tggctgtggt cgctccaccc cgcacgcctc cctggagcac aacacgacgt gggacctggt
     3481 cgaggacatg gagcggctcc gtcgacacct cggcgtcgac cagtggcaac tgttgggcgg
     3541 ctcatggggc tcgaccctgt cgctggtcta tgccgaaacg caccccgagc gggtctcgga
     3601 gctgatcctc cggggcatct tcctgctgcg caaggcggaa atcgactggt tctaccagga
     3661 gggctgcagc tggatttacc cggatgcctt cgaagcctac cagagcgtca ttccgccgga
     3721 ggagcgctcc gacatggtcg gggcctacta caagcggctg acgcaccggg accgccaggt
     3781 ccagctcgag gcggcgaagg cctggagcat ctgggaggcg acgaccctct ccctccaccg
     3841 cgacgccgag cgcatccgca atttcggctc cgacaattat gcgatcgcct tcgcccgcat
     3901 cgaatgccac tacttcatca accgcggttt tctcgcgagc gacggccaac tcatcgcgga
     3961 cgcgcaccgg ctttccggca tcccgggagt gatcgtgcac ggccgctacg atgtagtgac
     4021 accgctcaag aatgcttggg acctgaagaa ggcctggcca cgagccgatc tgcgcatcgt
     4081 accggattcc ggccatgcga tgaccgagcc cggcatcatc cacgagctcg tctccgcgac
     4141 ccgccgcttc agcccttgag ctcggcgcgg gcgcgttcga cgcagcgagg cgtgcttgcg
     4201 ccaccgatgc ggatcgctat agtccgcagc gcaattgcgg aggggtggcc gagtggttga
     4261 aggcgcacgc ctggaacgcg tgtaggcggg caaccgtctc gggggttcga atccccctcc
     4321 ctccgccatt ctcctgagca ccgtacttcg taagcgcctt caagaggcac tcacaaagat
     4381 cgttgtagag attgccagag caggcacgtt agctgctggt cgtttgcagg ccagctaaaa
     4441 gaagtcgtag ctccacatct ggtccgcacc cgttggcgcc ttcggcagtg ggcgcctcaa
     4501 ctcactacga gcgcatgagc gcgcgcgaga gcgaattgca acccgagtcg gtagctggct
     4561 cagagcggcg cggagcagcc gatgcaggga ggatcggctc cacgagagca agcgccgtca
     4621 cgagctgtcg gactggttga caattgagca ggggtggcat gcttagtctc ggagacaagc
     4681 agggcacgtt ggagccagag gccaaagccg ctgccgaggg aggccgcggg tggcggcatt
     4741 gccggacctt gcctccaggc aggaaaccgc agataaatcg gtaggatcgt ctggtccaga
     4801 aaatcgaccc gggaggaaat gatgaaacgc ctgcttgccg tgctctcggc attcgccttc
     4861 gccgttggcg tcagccccgc cgaggcccag tctccgaccg gcaaggtgac ggtctcgacc
     4921 tcctattcca aagacgtgac cgatcccgtc aagaaagcct tcgagaaggc tcacccgggc
     4981 gtgacgcttg atgtgcagaa ccgtaacacc aacgcgggcg tgaaatacct cgaggagacc
     5041 aggggcaaca accagacgga cctgttctgg gcttccgcgc cggatgcgtt cgaggtgctc
     5101 aagggcaagg gtcttctgca gaagtatgag cccaaggcga ccggcattcc ggagaagatc
     5161 ggcgccttcc cgatcaacga tccggacggc tactacttcg gcttcgcggc ctccggctac
     5221 ggcatcatgt ggaacgagcg ctacgccaag gccaacaatc tgcctgatcc gaaggagtgg
     5281 agtgatctcg ccaaggccgt ctatttcgac cacgtcgcca tggcggctcc ctcacgctcg
     5341 ggcactaccc atttgaccat cgaaaccatc ctccagggcg atggctggga aaagggttgg
     5401 cgcacaatca aggagatggc gggcaacttc cggcagatta cggagcgctc gttcggcgtg
     5461 ccggacgccg tcaactccgg ggaggtgggc ttcggcatcg tgatcgactt cttcgccttc
     5521 tcggcgcagg cttccggctt tcccatcaag ttcgtctacc cgacggtaac gacgatcgtg
     5581 ccggccaacg tggccatcgt cgccaatgcg ccgaacaaag ccggcgcgga agccttcatc
     5641 gagttcctgc tctcgccgga aggccaggag gttctcctgg agccgagcat ccgccggctg
     5701 ccgatcaacc cggccgtcta cgccaaggcg cccgcggatt atcccaaccc gttcaaggac
     5761 ccctcgctgg gagcccgcgt caagttcgac gtgaatatat ccgagaagcg cacggcagtt
     5821 gtcgatgcgt tgttcgacca gatgatcacg ttccagctcg acaacatcaa gagcgcgacc
     5881 aaggccgtgc acgaggccga ggcggcactg gccaagaagg acaatgcgga ggcccgtgcc
     5941 ctggtgaagg aagcgcgtga cctgatcgcc gcgatgccaa tcagcgagga gcaggcgtcc
     6001 tcgcctgaga ttgcaggcgc gttcaccggc ggcaagcaga agggcgcgcg ccaggccgag
     6061 ctcgagcagc agtgggccgc attcgccagg gagcactacg cccaagcgca ggcgaaggcc
     6121 gagcaggcct tgaagctcgc ccgctgacat attcgaggag gctccgattg gtggagcctc
     6181 ctcgtaaccc tctcagggaa acgacgatgg cgttggtgct gcctcgccgg gaaggcttcc
     6241 tttcggtttg gccgggtcag cttctggtcg cggcgctgat cgccgtcatc ctcctccttt
     6301 tcctcatcat acctgtcgcg acggtcatct acgtcgcctt taccgagaag gggacgggcg
     6361 cgttcacgct cgtcaacttt gtcgacttcc tgcacaccga tcttttcgtc aggtcgttct
     6421 ggaactcgat ctacgtgtcg gcgatggccg tggtgctggc ctccctcttc gccctcccgt
     6481 tggcgacgct tacgacgcgc ttcgagttcc gcggtgccct tatagttcag acgcttggct
     6541 tcatcccgct catcatgccg ccctttgtgg gcgcggttgc gatgcagctc ctgttcgggc
     6601 gcaacggcac cgtcaatctg ctcctcgatg actggctcgg tttcaagatc cccttcatgg
     6661 aagggctcaa cggggtcatt ttcgtccagg cggtgcacta cttcccgttc attctggtca
     6721 acgtctcagc tgcgttgcgc aatatcgacc gctcgatgga ggaagcggcg cagaaccttg
     6781 ggtgctccgg gttccggctg ttccgccgtg tcgtttttcc gctcgcgctg ccgggctacc
     6841 tcgcgggcgc gtcactcgtg ttcgtgaaag ttttcgacga cctcgccacg ccgctgctcc
     6901 tcaacgtcaa ggacatgctg gccccgcaag cctacctgcg cgtgaccgcg atcggcatcg
     6961 ccgacccgat gggctacgtg atctccgtgg tccttatcat cgtgtcgatc gttgcgatgt
     7021 gggcgtccgc ggctgcgatg cgggggcgcg actacgcgac ggtgcagcgt ggcggcggcg
     7081 ggctcgccaa gcgcaagatg gggctgtggg ggaatgtggc cgcctacgcc gtcgtgatac
     7141 tcatcctcct cctcgtgctc gccccgcaca tcgggctgct gctcctctcc ttcgcgacca
     7201 tctggtcgtt cagtccgctg cccgacggat tcacgctggc ccattacggg cgcgtcttcg
     7261 gcgagagctt catctacgtg aaaaatacgc tcctttacgc gtcgctcgcc ggattgatcg
     7321 acgtcgtcct cggtgtggcg atcgcctaca tcgtgctgcg cacccggttg ttcgggcgcg
     7381 agtggctcga ctgggccgcc tccgcggcgc tggcggtccc cggcgtggtg ctcggcatcg
     7441 gctatcttcg cactttctac ggggttcaac ttcctgatgg ccgccccctg gcaacgctgt
     7501 ggatcatgat cgtcattgcg ctggcgatcc ggcggctgcc gtacgcgctg cgcgcctgct
     7561 acgccgctct ccagcaaatc tccgcatcgc tggaggaagc agccgagaac ctcggcgcga
     7621 caaagtggcg gacggttcgc cggatcgtgg tgccactgat gatgggcggc atcctcgccg
     7681 ggttcatctc gagcttcgca acggccgccg tcgagctgtc ggcgacgctc atgcttgtgc
     7741 aatcggatac ggatgcgccc atcgcctacg gtctttatat cttcatgcag tcggccgcgg
     7801 gccgcgggcc gggcgcagcg ctgggcgtca tcgccgtggc cctggtggcg ctctgcacca
     7861 ttctctccca catcgtcatc gagcgcggac agcgagcgac gagccgtcgc tgacggcgaa
     7921 ttggcacttg gacatgcagc ggatgaaccg ggcagcttgg aatcacgaca gcgcaatcgc
     7981 acccgtcggc gttcgcatag agagcgtcga tgtgtcttat ggctccaatc acgtgctgaa
     8041 gagcgtgagc ctcgatatcg cgcccggcga gttcttcgcc tttctcggcc catccggctg
     8101 cggaaagaca accctgctgc gcctcatcgc cggcttcaac caagccgaca aggggcgggt
     8161 cctcgtcggc ggcgaggaca tttcgcgatt gccgccgtgg aagcgcgacg tcggaatggt
     8221 cttccagtcc tacgccctgt ggccgcacat gaccgtttgg cgcaatgtcg cttttgggct
     8281 ggaggaacgc cgcctgccgc gccaggagat agagaagcgc gtcgccgagg ctctggagct
     8341 ggtagggctc gccggcctcg ccgatcggcg tccgtcgcaa ctctccggcg gccagcagca
     8401 gcgggtcgcg ctggcgcgga cgattgcggt tcggcccaag gtgctcctgc tcgacgagcc
     8461 gctgtcgaac ctcgacgcca agctgcgcat gcaggtgcgg cgcgagctgc gtgatctgca
     8521 gcagcggctc ggcctgacga cgatcttcgt gacccacgac caggaggagg cgaacacgat
     8581 ctgcgaccgc atcgccgtaa tgaatgacgg aacagtgcag caggtcggca cgccgatgga
     8641 gctttacgag cgccccgcca atctgttcgt ggcgacgttt ctcgggactg ccaacgtcct
     8701 ggagggcgag ctcgtgggct cgggaccgga gcgcacctac gtcattggag agggcattgc
     8761 cctcccagtt cctccggaag ccaacgtcag tccgggagcg aagctcgtat tccgtccgca
     8821 ggaatgccgc atcggtgcaa gcggcgaagg catgctgcct ggcacggtgg cctatcgcga
     8881 gtttctcgga tcgacagtac gctacggcgt gcgggtcggc gcgaccgaga tcgccgtcga
     8941 tacgccattc caagccggcg acggcctcca cgagatcggc gacgcggtag gcgtgacgct
     9001 cccccttggc gctgccctgt ggcttgctag ctgatcgttc gccaagtttc cgcccaccgc
     9061 tcctccgctt cgatgacgat cggagccgtt ctttcggacc gatcgcttcg agactttcct
     9121 cgacgaggcg acggcattcc gctgcgggct tgccctgcag atgcagtccg ccggaagtca
     9181 gcctatttga tcccgctggc ggtaattctt ccaccgttta catcgccggc gccaaccttc
     9241 ctattaaaac gcaggcatcg tggttttttt aagtctcgta gatcctgctc gcgtcgccga
     9301 gacatgttgc ggccgcggtc gtggccttca acattgcgct tgaccggggt cctcgggcgg
     9361 ctacgcttcc tggcgagtgc aatccgtcaa gagcgagacc agttctgacg ataatgtctg
     9421 tcatcggcgg tcggcagccg tcgctaggac cggctgggcg tttccgtacc aatgcgcgag
     9481 cgtgatgctg atgccggcgg agctgcttga ggtcgaggac ctgacaatcc ggtttcaccc
     9541 cggcgggcct gcagtcgtcg accgtctttc gatttcactg acccagggcg aaacactgtc
     9601 gctggtcggc gaatcgggtt gcggtaagtc gctgaccgcg cttgcgatca tggggctgct
     9661 gccgcccggg gcgcggacga ccggccgggt tcgcctggaa ggtgaggatc tgcttgcggc
     9721 cggcgaacgg cgcctggccg atctcagagg aaatcggctc gcgatgatct tccaggagcc
     9781 gatgacgtcg ctcaacccgg cgttccggat cggcgagcag atcgccgaga gcgtcgtgcg
     9841 ccatcgcggg ctgcagcgca gcaaagcgag ggagcgggcg ctcgagatgc tccgggctgt
     9901 gcgtatcccg gcgcccgaga agcgcctcga cgcctacccg catcagctgt cgggcggcat
     9961 gcgtcagcgc gtgatgatcg cgatggcact cgtcaacgcg ccgaacatcc tgatcgccga
    10021 cgagcctacg acggcgctcg atgtcacgat ccaggcgcag atcctggcgc tcatacgcga
    10081 gttgcagcgc gaagctggca tggcgatgat cctcgtcacg catgacctcg gcgtcgtcgc
    10141 cgagacggcc gatcgggtcg ccgtgatgta tgccgggcgg atcgtcgaga cggcggctgc
    10201 gcccgatctc ttcgccgatc cgcagcaccc ctatacgctc gggctgatcg gctcgctgcc
    10261 ctcactcggc cggcgccagg gacggcttgc gacgattccc ggcagtgttc cgccgcctga
    10321 tcggttcccg ccgggctgcc ggttcgcgac gcgctgtccg ttcgccgacc ggcgctgcta
    10381 cgccgaggtg ccgcggttgc gcgcgatcgg tgccgaaggc cataaggttg cgtgtttcaa
    10441 ggcgccgatc gagcatctcg gcgtggagca ggcggcatga gctcccccgg cgcaggagag
    10501 acgatcctcg aagcacgcga ggtcgtgcgt tacttcggcg gccagcgcac gttccccttt
    10561 ggccgcgcgc cgctgatccg cgccgtcgac ggagtctcgc tcgcgatccg gcgcggcgag
    10621 acgttcgcca tcgtcggcga gtccgggtgc ggaaagtcga cgctcgcgcg cgttctcttg
    10681 aatttgattc cgccgacgag cggctccgtc gtgttcgagg ggcgcgagat cacaagcgcg
    10741 ccggccgagg agatgcgtcg gctgcgccgc aatatgcagc tcatcttcca ggacccgttc
    10801 gcctcgctca atccgcgcat gaccgtcggc gcgctgatcg gcgagccgct tgaagtgcac
    10861 ggcatcgcca agggtgccga gcggcggcgg cgggttgcca agatgctgcg cctcgtcggc
    10921 ctgtcgcccg actatgcgaa tcgctatccg cacgagttct cgggcgggca gcgccagcgg
    10981 atcggcatcg cacgcgcgct cgcgaccgag ccacgcctcg tgatcggcga cgagccggtc
    11041 tccgcgctcg acgtctcgat ccgcgcccag gtgatcaatc tgctcgaggc tctgaaagaa
    11101 gagctggcgc tgaccctcgt tctcgtcgcg cacgatctgg ccgtgatccg ccatacatcg
    11161 gacagggtcg ccgtgatgta tctcggcgag atcgtcgaaa tcgccgattg cgatgccctg
    11221 ttcgaggcgc cgcgtcaccc ctacacgcgc tcgctcatcg aggcgatccc ggtgccggtg
    11281 ccgggcgc
//
LOCUS       DASVDU010000233         4241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_214280, whole genome shotgun sequence.
ACCESSION   DASVDU010000233 DASVDU010000000
VERSION     DASVDU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214280"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF226_14955"
     CDS             complement(<1..478)
                     /locus_tag="VF226_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7075336.1"
                     /translation="MDTIKMPSVAAPAAALVAIAGFGLAAPAQADSGPYIGGSVGDAT
                     IQVDIPDEDNIGEVFEFDESDFAWKAFGGYNFDLAVIDLAIEGSYVDLGAPSVGDAAT
                     EIEVDVSGWDLFGLAGIELGPIGVFAKAGMINWDADTTLGELATGSDDGTDPAYGVG"
     gene            complement(617..934)
                     /locus_tag="VF226_14960"
     CDS             complement(617..934)
                     /locus_tag="VF226_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEX7075337.1"
                     /translation="MPGDSWATDQLDHPVFRAGAATSIIGILLLFRWLGRDYDAAVTV
                     GGFLGFGMSSMPVAMATMDSVALRYGPSPKAFLLITLAGSFFVDLANALVVKAFLALP
                     WLQ"
     gene            complement(909..1949)
                     /gene="glsA"
                     /locus_tag="VF226_14965"
     CDS             complement(909..1949)
                     /gene="glsA"
                     /locus_tag="VF226_14965"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX7075338.1"
                     /translation="MNTRRWQCGIAIATLLVLGGTARAQSDADIQSALDAAYAKYQDL
                     NEGANADYIPALAKVDPDIYGIALVTADGDVYTAGDVESEVSMQSISKVFTLAKVMEE
                     QGPESIADTIGVDATGMRFNSIVSVEFAQKALGGPEMNSLVNPGAIATTSMVQGSTRD
                     EIWNSLLSFYSDFAGRPLSVDEEVFKSESDTNQRNQAIGYLMYAYGYIKNAPLRATDI
                     YTEQCSVSVNARDLATMAGTLANAGKNPVTGKQVMKADNVPELLAVMATAGLYDDSGK
                     WLYRTGLPGKSGVGGGLIAVSPGKFGIAVISPPLDEAGNSVRAQKAIADISDALGGNP
                     LAAGNAGGQLGD"
     gene            complement(1946..3052)
                     /locus_tag="VF226_14970"
     CDS             complement(1946..3052)
                     /locus_tag="VF226_14970"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7075339.1"
                     /translation="MLRAARRDFCLTTRTGRVVRAWALSATAFCIAAFPVRLPAAEPV
                     LNDSHFHLTNYVQQGLEISDFLEMMGDKVGRVAIFGIPLQQTWAYSNTGDVAPTYYLQ
                     TDAPLYYYSFVDAAIATAYLSLPQRDRERFDPMITGFNPADMYAVDHIRRVLLTFPGV
                     FTGIGEFSIHKEFVSAKIAGGPASLTDPALDRILDFAGEAGLVVLIHNDVDMPFPKPG
                     QDPYSARQLGELLRRHPDTTVIWAHMGLGRVVHPVKDMAGIMEHVLADPGLAHVYIDL
                     SWTETAKYLVASPDTIAVAAKLINRYPDRFLFGTDEVAPPDQESYLKTYALYSPLLER
                     LDEGARSKFLKGNYEKVFNAARRKVRAWEQENRQ"
     gene            complement(3066..>4241)
                     /locus_tag="VF226_14975"
     CDS             complement(3066..>4241)
                     /locus_tag="VF226_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075340.1"
                     /translation="GGAGFKLADTPHGDVNFSAWAYIRYLNQTSLDDTYTDSFGRTTT
                     LDLRNDMQVNKINLYFKGWVFDPDFHYNLWSWTTNTSQGEPAQVVIGGDLGYRFSDKL
                     DLRFGVLPLPGTRSLRGQHPYWNKVDHRTIGNEFFRPSYTTGIIANGQLAEGVYYRAA
                     IANNMSALGVSAAQLDDEFNTVSAAIWWMPTGEYGTAAGYSDFDYHEEVATQFGMGVT
                     RSRESRQNQPGTEDPENTQLRLSDGTIIFRPDAFGTGGTILEATYLMTAVDAGVKYRG
                     LALEGEYYFRWIDDFEITGTIPVTELYDHGFELQASAVLLPETLSTYVAGSKIFGEYG
                     EPWDLGVGLNWYPFRQRLMRVNAELLYLRDSPVGYSSVPFVVGGNGTVFQANLEARF"
BASE COUNT          744 a         1315 c         1364 g          818 t
ORIGIN      
        1 ccccgacgcc gtaggcggga tcagtgccgt cgtcgctgcc ggtcgcaagc tcgccgagag
       61 tcgtatccgc atcccagttg atcattccgg ccttggcgaa aacaccgatc ggtccgagct
      121 cgatgcccgc gagcccgaac aggtcccagc cgcttacgtc cacttcgatc tccgtcgcag
      181 cgtcaccgac cgaaggcgca ccgagatcca cgtagctgcc ttctatcgcc aggtcgatca
      241 cggcgaggtc gaagttgtat ccgccgaacg ctttccaggc aaagtcgctt tcgtcgaact
      301 cgaagacttc accgatgttg tcctcgtcgg gtatgtccac ctggatcgtg gcatctccca
      361 cgctgccgcc gatgtagggg ccgctgtccg cctgggccgg cgcggcaagg ccgaaaccgg
      421 cgatcgcaac aagggccgcg gccggagcag caacggaggg catctttatc gtatccatgg
      481 caaggtctcc tcggtgatga ggggcaaatg aattcaactg cttaggcgca cccggacgga
      541 tgcaggggtc gtgatatgca tattgtagtc cagtatctgc ggtcacggga ttggaccatg
      601 gcctgattgc cttgcctcat tgcagccagg gcaaggcgag aaatgccttg acgaccaggg
      661 cattggccag atcgacgaag aacgatccgg cgagggtaat gagcagaaac gccttcggcg
      721 agggcccgta acgcaatgcg acgctgtcca tcgtggccat cgctaccggc atcgagctca
      781 taccgaatcc gagaaaaccg ccgacggtga cggccgcgtc gtaatcccgg ccgagccagc
      841 gaaacagcag gaggatgccg atgatcgaag tcgccgcccc agctcggaaa actggatgat
      901 caagctggtc agtcgcccaa ctgtcccccg gcattcccgg cggccagcgg attcccgccg
      961 agcgcgtcgg aaatgtcggc aatggccttc tgcgcgcgga cgctgttccc tgcctcgtcg
     1021 agcggcggcg agatgacggc gatgccgaat ttgcccggtg acacggcgat gagacctcct
     1081 cccacgccgc tcttgccggg cagcccggtg cgatagagcc acttgcccga atcgtcatac
     1141 aggccggccg ttgccatcac cgcaagcaac tccgggacat tgtcggcctt catgacctgc
     1201 ttcccggtca cgggattttt gcccgcgttg gccagtgtgc ccgccatcgt cgcgagatcc
     1261 ctcgcgttga cactgaccga gcactgctcg gtgtagatgt cggtcgcccg cagcggcgcg
     1321 ttcttgatgt agccgtacgc gtacatgagg tagccgatcg cctggttgcg ctggttcgta
     1381 tccgactccg atttgaagac ttcctcgtcg acggacagcg gccgtcccgc gaagtcggag
     1441 tagaaagaca ggaggctgtt ccagatctcg tcacgcgtcg aaccctgcac catgctggtg
     1501 gtcgcgatcg caccggggtt gacgagcgag ttcatctcgg gaccgccgag agccttttgt
     1561 gcaaactcga cggacacgat ggagttgaag cgcatgccgg tcgcatcgac gccgatggtg
     1621 tcggcgatcg actccggccc ctgctcttcc atcactttgg cgagcgtgaa caccttggat
     1681 atcgactgca tcgatacctc cgactcgacg tcgcccgcgg tgtagacgtc gccgtcggcg
     1741 gtgaccagcg cgatgccgta gatgtccggg tcgacttttg cgagcgccgg gatgtagtcc
     1801 gcgttggcac cctcgttgag gtcctggtat ttcgcgtagg ccgcatccag tgccgactgg
     1861 atatccgcgt cggactgggc ccgcgcggtc ccgcccagga ccagaagagt cgcaattgcg
     1921 atgccgcatt gccaccgtcg tgtgttcatt gccgattctc ctgttcccag gcgcgcactt
     1981 tccggcgtgc cgcgttgaaa accttttcgt aattgccttt caggaatttg ctgcgcgctc
     2041 cctcgtcgag tcgttcgagc aatggcgagt agagtgcgta agttttgaga tagctttcct
     2101 ggtccggcgg agccacctcg tcggtgccga acagaaaccg gtccgggtag cgattgatga
     2161 gctttgccgc gacggcaatc gtgtcgggac tcgccaccag gtacttggcg gtctccgtcc
     2221 acgacaggtc gatgtagacg tgtgcgagcc cggggtctgc cagcacatgt tccatgatcc
     2281 cggccatgtc cttgaccgga tgcacgaccc ggccgaggcc catgtgcgcc cagatgaccg
     2341 tcgtgtcggg atggcgccgc agcagctcgc cgagttgccg cgccgagtac gggtcctgcc
     2401 cgggtttcgg gaacggcatg tcgacgtcgt tgtggatcag gaccacgaga cctgcttcgc
     2461 cggcgaaatc gagtatgcgg tcgagtgccg ggtcggtcag gctcgccggg ccgccggcga
     2521 tcttcgccga cacgaattcc ttgtggatgc tgaactcgcc gatcccggtg aacactcccg
     2581 ggaaggtcag gagcacgcgg cggatgtgat cgacggcgta catgtcggcg gggttgaagc
     2641 cggtgatcat cgggtcgaag cgctcccggt cccgctgcgg cagcgacagg taggcggtgg
     2701 cgatcgccgc gtccacgaag gagtagtaat agagaggcgc gtcggtttgc aggtagtacg
     2761 tcggcgcgac atcgccggtg ttggagtaag cccacgtctg ctgcagcgga atgccgaaga
     2821 tggcgacccg gcccaccttg tcgcccatca tctcgaggaa gtcggagatt tcgagccctt
     2881 gttgcacgta gttcgtgagg tggaagtggg agtcgttcaa cacgggctcg gcggcgggca
     2941 aacgcacggg aaatgccgcg atacagaacg ccgtcgcgga aagtgcccag gcccggacaa
     3001 cccgcccggt gcgggtcgtc aaacagaagt cacgtctggc cgctcgcagc atgtgccggg
     3061 ttccttcaaa aacgagcttc caggttggcc tggaacaccg tgccgttgcc gccgaccaca
     3121 aacggcacgc tggaatagcc gaccggcgag tccctcaggt acaggagctc ggcgttgacg
     3181 cgcatcagcc gctggcggaa gggataccag ttgagcccga cccccaggtc ccagggctcg
     3241 ccgtactcac cgaagatttt ggaaccggcg acataagtgc tcagggtctc cggcaacagg
     3301 acggcggacg cctgcagctc gaacccgtgg tcgtacagtt ccgtgaccgg gatcgtgccg
     3361 gtgatttcga aatcgtcgat ccagcggaag tagtattcgc cttccagagc gaggccgcgg
     3421 tacttcacgc ccgcgtccac cgccgtcatc aggtacgtcg cctccaggat ggtgccgccc
     3481 gtgccgaatg cgtcgggccg aaagatgatc gtgccgtcgg acaggcgcag ctgggtgttc
     3541 tccggatcct cggtgccggg ctggttttgc cgggattcgc ggctgcgcgt aacgcccatg
     3601 ccgaattgcg tggccacctc ctcgtggtag tcgaagtcgc tgtagcccgc tgcggttccg
     3661 tattcgccgg tcggcatcca ccagatcgct gccgagacgg tgttgaattc atcgtcgagc
     3721 tgtgccgcgc tcacgcccag tgcactcatg ttgttcgcga tcgcggccct gtagtagacg
     3781 ccctccgcga gctggccgtt cgcgatgatc ccggtggtat agcttggccg gaagaactcg
     3841 ttgccgatcg tgcgatggtc caccttgttc cagtaggggt gctggcctcg caagctccgc
     3901 gttccgggca aaggcagcac tccgaagcgc aggtccagct tgtcgctgaa gcggtacccg
     3961 aggtccccgc cgatcacgac ctgtgccggc tccccctggc tcgtattcgt ggtccagctc
     4021 cagaggttgt aatgaaagtc gggatcgaag acccagccct tgaagtagag gttgatcttg
     4081 ttgacctgca tgtcgttgcg caggtcgagc gtggtcgtcc tgccgaacga gtcggtgtag
     4141 gtgtcgtcga ggctggtctg gttcaggtag cggatgtacg cccaggcgct gaagttgacg
     4201 tcgccatgag gtgtgtccgc gagcttgaag ccggccccgc c
//
LOCUS       DASVDU010000234         2658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_198052, whole genome shotgun sequence.
ACCESSION   DASVDU010000234 DASVDU010000000
VERSION     DASVDU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198052"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VF226_14980"
     CDS             complement(<1..350)
                     /locus_tag="VF226_14980"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7075341.1"
                     /translation="MTKIGYAAMLEQFHPNDLLDWCAQAEAAGFDAGFQVSEHFHPWT
                     PQQGQAAFAWSFMGALGERTSLPFGTAVTCPGFRYHPAVIAHAAATLGAMFPGRFWLG
                     LGAGEALNEHVVTDT"
     gene            499..2184
                     /locus_tag="VF226_14985"
     CDS             499..2184
                     /locus_tag="VF226_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX7075342.1"
                     /translation="MESTTGAAYLARALHAYGVSAVFLVPTMLSKTLFEMEQLTDIKR
                     IVTHSEKAAAYMADGYARTKGSPGICMAQTIGSANLAAGLRDAFLGASPVIAMTGGPY
                     PWSRHRAQYQEIEDRPLFKEVAKASYVVDTVDRLPGLLSHAFNVATQGRPGPVHLELP
                     GHSGELIDEMTMTAQLPELHEIHVPRFRVHPDPAAISGAVERLLRASRPVIVAGGGTK
                     ASGAAPMLREFAELLGLPVATSLNGKDLLPADHPLHVGIPGLYPSGNANAVVAAADLV
                     LFIGSQAGSQVTLNWQIPPQTTDTIHVDIDPDQLGRHYPDSLPVLGDAKIVLELLTAR
                     LAGEASLPDFAAWRGRWSELVASWKKQAAPILASEAEPIRPERLTSELSKALPPDAIV
                     VADTGHAGMWAGGYLDLKHDTQSYLRAAGSLGWALPASIGAKIAAPDRPVAMFTGDGG
                     FWYHLSEIETAARWGVGVVMVVNNNNSLNQEIRPFTAAYGGTLHGRHHELWHFRDVSF
                     KAVGEAMGIPAYRVEKPSDLSIAFDAAFAEHGPVIVEVVTDMEIMAPRGSVPA"
     gene            2311..>2658
                     /locus_tag="VF226_14990"
     CDS             2311..>2658
                     /locus_tag="VF226_14990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HEX7075343.1"
                     /translation="MYDTRVRGGTVYTPTGPITADVLIEGEQIAGLVAPDYEADAREE
                     IDASGKVVLPGIVDLHAHTRTPGLSHKEDFLTASQAAANGGITTFVDMPNVEPPTDSA
                     ELLIEKRKIAERDC"
BASE COUNT          485 a          842 c          851 g          480 t
ORIGIN      
        1 gtgtccgtca ccacgtgctc gttcagcgcc tcgcccgctc cgaggcccag ccagaatcga
       61 ccggggaaca tggcacccag cgtcgccgcc gcatgggcga tgacggccgg gtggtatcga
      121 aagccgggac acgtcacggc ggtgccgaag ggcagcgacg tgcgctcacc cagcgcaccc
      181 atgaagctcc acgcgaacgc cgcctgaccc tgctgagggg tccacgggtg gaagtgctcg
      241 ctcacctgga agccggcatc gaagccagcc gcctccgcct gagcgcacca gtcgagcagg
      301 tcgttggggt ggaactgctc gagcatggcg gcatacccga tcttggtcat tcatttctcc
      361 aatgcgtcct ggcttgagca aaaaattccg agctgctcac tgcccggaag aaggctcatt
      421 gtaagagtcc acgcgagatc ggtcgcttcg ggtgtcaatg atattgttct tgccgtttca
      481 gttcgacgag gtgcttgagt ggaatcgact acgggagctg cctatctcgc gcgcgcgctg
      541 cacgcctacg gcgtctcggc cgtgtttctg gttccaacca tgttgagcaa gacgctcttt
      601 gaaatggagc agctcacgga tatcaagcga attgttacgc attctgaaaa ggcggctgcc
      661 tatatggccg acggctatgc gcgcaccaag ggctcaccag gcatctgcat ggctcagacc
      721 atcggcagcg ccaatcttgc tgcgggcctt cgcgacgcat ttctcggagc gtcgccggtg
      781 atcgccatga cgggagggcc ttacccgtgg agccgtcacc gcgcgcaata tcaggagatc
      841 gaagaccgtc cattgttcaa ggaagttgcg aaggcgagct atgtggtcga taccgtcgac
      901 cgcctaccag gcctcctgtc gcacgccttc aacgtagcca cccagggacg gccggggccc
      961 gtccacctgg agctgcccgg gcactccggc gagctgatcg acgagatgac gatgaccgcc
     1021 cagctgccgg agctgcacga gattcacgtt ccccgatttc gggttcaccc cgacccggcg
     1081 gcgatctccg gtgcggtcga gcgcttgctg cgcgcctcac gcccggtcat cgtcgcgggc
     1141 gggggcacga aagcctccgg cgcggcgccg atgctgcgcg agttcgccga actgctcggc
     1201 cttccggtcg cgacctcgct gaacggcaag gatctgttgc cggcggacca tccgctgcat
     1261 gtcgggatcc cggggctgta tccgagcgga aacgcgaatg cagtcgttgc agctgccgat
     1321 ctggtgctgt tcattggcag tcaggcaggc agccaggtca cgctcaactg gcaaattccg
     1381 ccacagacga ccgatacgat tcatgtcgac atcgatcccg accagctggg ccggcactac
     1441 ccggattcac tgccggtgtt gggcgacgcg aagatcgtcc ttgagctcct cactgcgcgg
     1501 ttggcggggg aggcctcgct ccctgacttc gccgcgtggc gcggccggtg gtcggagctg
     1561 gtcgcctcgt ggaagaagca agcagcgcca atcctggcat ccgaggcaga gccgattcga
     1621 cccgagcgac tcacgtccga actgtcgaag gctcttccgc cggatgcgat cgtggtcgcc
     1681 gacaccggtc acgccggcat gtgggcggga ggctatctcg atctcaagca cgacacgcag
     1741 tcatacctgc gtgcggcggg ctcgctcgga tgggccttgc cggcgtcgat cggggcgaag
     1801 attgccgccc cggaccgccc ggtcgcgatg tttaccggcg acggcggatt ctggtatcac
     1861 ctctcggaga tcgaaacggc cgccagatgg ggcgtgggag ttgtcatggt agtgaacaac
     1921 aacaactccc tgaaccagga gatcaggccg ttcaccgcgg cgtacggtgg cacgctccac
     1981 ggtcgtcacc acgagctgtg gcacttccga gacgtgagtt ttaaagcggt gggcgaggcc
     2041 atgggaattc ctgcctatcg tgtggagaag ccttcagatc tttcaattgc attcgacgcc
     2101 gccttcgccg agcacggccc ggtcattgtt gaagttgtaa cggacatgga gatcatggct
     2161 ccgaggggca gcgttccggc gtgatgccca cgcagtgcgc gttcttgttt gaaagtatgc
     2221 gcaggcaaat ggccgctgcc ccgctcgact gaaaaaatgc tagagttgtc agcccaatag
     2281 ctcggctgtt tgcctattag tggagaaggt atgtacgaca ctcgagtgcg cggtggcacc
     2341 gtctataccc cgaccggtcc gatcacggcc gacgtgctga tcgagggcga gcagattgcc
     2401 gggctggtgg cgcccgacta cgaggccgat gcccgtgagg agatcgacgc cagcggcaag
     2461 gtggtgctgc cgggcatcgt cgacctgcac gctcacaccc gcacgccggg cctcagccac
     2521 aaggaagact tcctgaccgc ctcgcaggcg gcggccaacg gtggcatcac caccttcgtc
     2581 gacatgccca acgtcgagcc gccgaccgac tcggccgagc tattgatcga gaagcgcaag
     2641 atcgccgagc gcgactgc
//
LOCUS       DASVDU010000235        27695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1567209, whole genome shotgun sequence.
ACCESSION   DASVDU010000235 DASVDU010000000
VERSION     DASVDU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 27695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27695
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567209"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..570
                     /gene="hisI"
                     /locus_tag="VF226_14995"
     CDS             121..570
                     /gene="hisI"
                     /locus_tag="VF226_14995"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX7075344.1"
                     /translation="MTSASGTSSNLFAPRGSAEVIERGDVFQPKFGPDDLLPAIITDA
                     EAGAVLMFAWMNPEALALTIETGLAHFWSRSRQRIWKKGEESGNLLRVAEILTDCDQD
                     VLWLKCHVEGDGKACHTGARSCFYRALSPHRTEDGAVRLVPAASTEG"
     gene            781..1758
                     /locus_tag="VF226_15000"
     CDS             781..1758
                     /locus_tag="VF226_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7075345.1"
                     /translation="MAKVKIGLALGCGAARGWAHIGVLRALLEAGIVPDIVAGTSIGA
                     VVGGCYAAGRLDEVEEFACSLTRRRVFGYLDFNMSGSGLITGQRLRDRLINHLGDTTI
                     ESLNPQFTAVATEIGTGHEIWLSRGRLVDAIRASYALPGIFKPVNVGGRWLFDGALVN
                     PVPVSVCRALGARYVIAVNLNADMCGRGTVVPFLEAGTQDTLLIEANGVPKKNGRAAM
                     KLLRRQFFGPDETAPGISTVVMDAFNIVQDRIARSRLAGDPPDFMISPRLAGIGPFDF
                     HRAAELIERGTAAAHREVEDIAREIEVRRMFEPRVEEEREGAVVEAGRE"
     gene            complement(1884..3101)
                     /locus_tag="VF226_15005"
     CDS             complement(1884..3101)
                     /locus_tag="VF226_15005"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7075346.1"
                     /translation="MTRGLMKTASSIAVAAAAGLFATSAMAADLGGNCCADLEERVAE
                     LEATAARKGNRVVSLTVYGAVNQAIVWHDDEFGYREGKATVRDNGAYMSRFGFKGEAQ
                     IRPDLTAGYVIEIGLSNNDFGTGSAADSFTIRHNYLYLNSQTFGRVNLGQTSQVTDGL
                     YEINLATPYVTVGGMDEAEFSLHSHFTDIFVNGFDGGRKQGVYYTTPSLAGFLLSVGY
                     SHDTGELDEGIFPHLIGGDNVHDADDYWEVALRYAGEFNGVRVAAGVGYRAKEDNPTV
                     GAAVDEETILGSGSIMHVPTGLFLSGGYATTDSDDDLDDKDGFWVMAGVEKNYFGYGN
                     TTLFAEYSEVNFDTAPVDDKGRYWGAGIVQNIDAAAMALYLNWRNYEVEDAGVFDADE
                     DANVVHAGAIIKF"
     gene            complement(3644..5218)
                     /locus_tag="VF226_15010"
     CDS             complement(3644..5218)
                     /locus_tag="VF226_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075347.1"
                     /translation="MLLSAPTEQHQVEMEMIGGLIKTTSRFAIAAAASVIATSAMAAD
                     LGGNCCADLEERVAELEATAARKGNRRVSLTVYGQVNEGILWWDDGEETNAYVVTNEN
                     SRTRFGFRGDATISPEWKAGYLLEIGVRIANSANVNQFADDLVGGTSSLDLRHSAWWI
                     ESNRLGRVWVGHTSMATDGITEINLSNAAQIGAPDVNNWGGGMFLRLSNGTLLAVAPF
                     AGLQWNDILSQANANVGDGDRRNLVRYVSPTFSGFTFSASWGEDDFWDAALRYANEWN
                     GIRVAAGIGYQQWTDGDATAVLPTDIFGITGFAVTGTGSNNDRGCADLDFVDPVFGIG
                     VTGDSDVDCWAVGMSASAMHVPTGLYIAGAYGFLKDENRRELFALRALEGNLLLPDVD
                     DRDEFWYVQAGIEKNFFGLGNTTIYGEYFQADTGGGLAGGNVNDLGFLAGTLPLGANF
                     DTFGAAIRSSQVTVWGFGIVQTIDAAAMDLYAGYRNYSGDFDAVVNVGASPFGTRLNL
                     DVEDYQAVFTGGIIRF"
     gene            complement(5774..6205)
                     /locus_tag="VF226_15015"
     CDS             complement(5774..6205)
                     /locus_tag="VF226_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7075348.1"
                     /translation="MNVAAILKSKGRAVATARPETTLQAAAEKMSAKRIGSLVVLGAS
                     GTVVGIISERDIIRAIAERGRDCLEQPVSEFMTRQVVVCSEADTLDQLMATMTAGRMR
                     HIPVLEDDALVGIVSIGDVVKHHIAEVEMEASALRTYLLAG"
     gene            complement(6399..7127)
                     /locus_tag="VF226_15020"
     CDS             complement(6399..7127)
                     /locus_tag="VF226_15020"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7075349.1"
                     /translation="MRREPIFNAPYVILGLLAAFALLHVARGFLSDIDDAYLVLTLGF
                     IPARYGDSPFVLPGGEISAVTSFITHMFVHGDPLHLLINSAWLLALGTPLVRRIGSLR
                     FLLFSAICGIAGALTFLAINPGLPVPMVGASGAISGMMGGLMRFLFNAMDRGGAAVIP
                     EGAREVPRMNLGQMLHDRRVLITIAIWVILNFAFAIGFGDLAETGGIAWEAHLGGFFA
                     GLLIFGAFDRAAEPTLEEADPPLR"
     gene            7315..8004
                     /locus_tag="VF226_15025"
     CDS             7315..8004
                     /locus_tag="VF226_15025"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7075350.1"
                     /translation="MAWGDAGRERAANMAERPGAQVDRMKNDTSRVLFHYWNAIRGSR
                     RAPRRFEIEPARISRILPEAFILEQRDVDTYRFRIAGTRLCELFGMELRGHNFLELWE
                     DGDRFPVKRRLACVAEEGAVGLFEFEASVSVRESASFEVLIVPLIQKDAAIDRFLGTM
                     SPISEPLWRGVERLERCHLLRHELIWPEPASGRIVRAPLPEVRDARLVRVAHRSFRVY
                     EGGLSRESGKR"
     gene            8196..8834
                     /locus_tag="VF226_15030"
     CDS             8196..8834
                     /locus_tag="VF226_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7075351.1"
                     /translation="MTWTPITATVPDGADGRPAEAPLASPRERRLHKRYKIVVPGRFM
                     RLNKEEHPCRLRDISCSGAAITSPVSLEIGERIVVYFEHIGGLDGIVVRTFEGGFAIE
                     LCVTQHKREKLADQLARLANRDGKPAPRRHERFTINNTTTLRLDEDISIQVRVLDVSI
                     SGASVETEARPALGSEVVLGKLRAKVVRHHMEGLGLEFMDIQNPDALRRSIR"
     gene            8996..9634
                     /locus_tag="VF226_15035"
     CDS             8996..9634
                     /locus_tag="VF226_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEX7075352.1"
                     /translation="MRVKALVLIVVSGLLHAGSAAADNSKIRAAEHSSLYMRVFGQAI
                     PPFGFIQFCEAWPDECKAGHWRDRRFQATPERLSELDEVNRYVNRRIRPTTDQELFGV
                     TERWVIPTDQGDCEDYALLKRKILIERGWPPSSLLMTVVRDELGEGHAVLTARTAQGD
                     FILDNKVDEVMLWYVKPYEYIMRQSFLDPKVWVSLNPQAYSPAAIAVVRNQD"
     gene            complement(10078..14124)
                     /locus_tag="VF226_15040"
     CDS             complement(10078..14124)
                     /locus_tag="VF226_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7075353.1"
                     /translation="MSDSDPCCSAAPSRGVGEYLKGLMLLVFGLLLLTTAAEPARALE
                     PIEVRPDQDRIEITGQGEIHTGQGDTLQVDTASGPDGVSGRMSIRASTPGTNPNWLVF
                     ALRNPTDKPIERWLTAERYTVIGSSVIWPDLDTRRIEAVTPSLGYLPDRVRSDRADIF
                     RITLEPGQTITYAAELASERIPRVFLWKSLEYELNARDRQLFNGIMLGITGLLAIFLT
                     AVFAANQKAIFPAAALVAWCVLGYLCVDFGFWHKLFHLKAEDNAVYRAATESAIAASI
                     VIFVSVFLRLSSWHGFIRMVASVWIAAQLALVFVAVIDPRLAATFARASFAVIGSLGA
                     LLTLYLAIRGQDRALALIPTWLLFLVWIFAAAVTLTGQLSGDIVVSALAAGLVLIVVL
                     MGFTVTQFAFRSFEPVYGAAPSEMQLRSMAIDGSGAAVWEWLARRDEIKVSPVIEASL
                     GLTAGELSTKVDDFAKHLHPADRERFRLVLWGVQERNGGPIHIELRMRHTDNSYRWFE
                     IEAASVPHSDRRALRCVGLMRDITEAKRAQERLMHDAVHCSLTGLPNKALFLDRLGVA
                     IMRAKVEQQLRPTVFFIDIDRFKNVNASLGLIVGDSILLTVARRLMRHVGPQDTLARV
                     GGDQFAMLLVSHHEPRELALLAERVRRSLRSPIKIAGQEISLTTSTGIAVMDGDLDAE
                     ELLKQAEIAMYRAKRSGADRIEMFMPDKRREPDDRVVLEGDLRRALERNQIHIEYQPI
                     IYLPTEELAGFEALVRWDHPKLGRLSPAEFVPIAEKSDLIVKLGSYVLTRAAEEAAKW
                     QKELPRGDNPVFVSVNVSSRQLFRQDLIQEIRHILGRALVPPRALRLEITESVAMENP
                     EQAVHVLELLRSAGAGLSIDDFGTGYSSLAYLQRFPFDTIKIDQGLVQAINGNGPGAA
                     IVRSIVALAHELGKKVVGEGIEVAEDANFLRGLGCEYGQGYYYGEAMSDREALQLVRT
                     IRKSERKLQRSGLFRPRSLSRRRKKRTEVSAPAPATAEAPALTPAEASQKANVTAPDS
                     TLDRPAEPTKSRKEKGKPEAGRRGLQAAKQLLARTSGKRAGGAKSDGADPAAGPSAGD
                     STSKQKTGSPVAVPPLPLQPKGSHAAPPPAGAPAGAQGESSARTQPPSRGVPRTQPPP
                     VRQQTQPPPPPQDGAQQPVRSAPPPPRPSQAKQPPGPPPVPPKLTPGPKTAPPHPRQP
                     AVAASPQQPRPGPTTAPPPPSMRKGGPPARPTAPPVARQPGPPPPAAPSSVTAPPRQP
                     AHTAPPPPNGAAAGRTPPPAPPPGARQNAPQFPSLPPAIAESLAKLAGMPIADASNAR
                     PADKSGNGAASGPAEPVRPRVEAKGSSH"
     gene            complement(14114..14734)
                     /locus_tag="VF226_15045"
     CDS             complement(14114..14734)
                     /locus_tag="VF226_15045"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7075354.1"
                     /translation="MAFLRSGLGSEWLPLVRAGSIYLRPPHMGDYAAWAELRAKSRDH
                     LVPWEPQWTRDELSRDAFRRRIRHYQRELRDDMGYAFFLFQEADDTLLGGLTLSNVRR
                     GVTQAAAVGYWVGKPYAGRGYMTRALAGAVRFAFDELKLHRVEAACMPNNLASIRVLE
                     RNRFQREGLARRYLKINGVWQDHLLYATLADDPRDDFGGSGIRNER"
     gene            complement(14753..16021)
                     /locus_tag="VF226_15050"
     CDS             complement(14753..16021)
                     /locus_tag="VF226_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7075355.1"
                     /translation="MTTAVTRLRNGLRVVSHQMPHLETISLGVWVATGARHEREEEHG
                     ISHLLEHMAFKGTRRRTARQIAEEIEQVGGELNAATSLEMTAYYARVLKGDEAIALEI
                     LADILQNSTYAEAELEREREVILQEIASIQDSPDEIAYDLVQDAAYPGQAVGRPIIGT
                     RESVTRLTSEDLRNFLRTHYLAPQMVISAAGAIDHGTLVRHAEALFGGLYGGESQDGS
                     VARYVGGQRTSRKLFEQSHLLLGFEAPSYRDQEFYAAQVFSGLFGGGMSSRLFQAARE
                     NRGLCYAIYSTAWGMADCGMFDIHAATGPEQMGELIDVIGTELFNIAATPPSDAEVAR
                     AKAQLKAGLLTGLESSSARAEQMARQLMFFDRLIPPAELVQRIEDVTPERIRAIAERV
                     ARSALPSIAVVGAGRRSADYAQRAERRLAG"
     gene            complement(16018..17427)
                     /gene="thrC"
                     /locus_tag="VF226_15055"
     CDS             complement(16018..17427)
                     /gene="thrC"
                     /locus_tag="VF226_15055"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7075356.1"
                     /translation="MKYISTRGLAPELGFEDVLLAGLARDGGLYVPETWPKLDNGAIA
                     SLAGRPFSEVAVEVIHPFTGGALSRDELLAMAKSAYATFGHPAVTPLVQVDSNLWVLE
                     LFHGPTLAFKDVAMQLLSRLMDHVLKRGGQRATIVGATSGDTGGAAIEAFRGSKAVDV
                     VILYPAGRVSDVQRRMMTTPREPNVHAVAIEGTFDDCQALVKGMFNDHAFRDRMRLSG
                     VNSINWARIVAQVTYYFAAAVALGAPHRSVSFAVPTGNFGDIFAGYVARQMGLPIERL
                     VIGSNVNDILPRTVATGIYEKRGVTATTSPSMDIEVSSNFERYLFEAQGRDAALVRNQ
                     MTALAQSGRFEVRGGPRLIAQDFSAASASETEVAETIRRVRQSSGYTAEPHTACGLIA
                     AQRCLPSRGAPQVVLATAHPAKFPDTMETILGERPGLPPRLASLMTDEERSVRLPNDL
                     AAVERFVEERVGAGVEQQP"
     gene            complement(17509..18351)
                     /locus_tag="VF226_15060"
     CDS             complement(17509..18351)
                     /locus_tag="VF226_15060"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX7075357.1"
                     /translation="MAQRLRGGGLVALTVATLAALVLLIGLGNWQWQRKAWKEELIAT
                     MEARASAEPLPPERWSTLTCSPVHEVGLAGSCEFMTVRLVGKFDNPDERHVFTTAPAG
                     ASPGGAGYWVFTPFELAGGNGRIYVNRGFVPEARKDPQTRPEGQIQGETELIGQLRTA
                     EQAGMFTGESNPAANIWFLRNPREFLTRSGVEGARLARWQGSGPSGLDFYVDQIGPTP
                     PGGLPAPRPSRLELPNRHLEYALTWWGLALTLLAVYIAFVISRLRGSESRVLDPRPPS
                     RSPR"
     gene            complement(18351..18737)
                     /locus_tag="VF226_15065"
     CDS             complement(18351..18737)
                     /locus_tag="VF226_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX7075358.1"
                     /translation="MAQDDQLGTALRAGLLGRCPRCGEGQLFDGPLTLKLRERCETCG
                     LSNGFADSGDGPAVFVIIILGFLIMGAALIVEFKLSPPLWVHVVLWFPIAALLGYGLL
                     RPMKGTLIALQYRNRAEEGRFDQDGR"
     gene            complement(18870..19805)
                     /locus_tag="VF226_15070"
     CDS             complement(18870..19805)
                     /locus_tag="VF226_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7075359.1"
                     /translation="MADSHAKHHDYHLVDPSPWPLLASLSAFVLAVGLIIWMRSIGGG
                     SGLFGVQGSTVFFIGAAALVAVAFMWWRDVVREAHKGDHTPVVQLHLRYGMILFITSE
                     VMFFLAWFWAYFDAALFPGGVSPLPITMPDGSIAPSDTSIIGLVERNELTGGQWPPIP
                     SDSFQSTFDPWGLPLVNTLILLLSGTTVTWAHHALLQNDRKGLVWGLTLTVCLGLMFT
                     LLQAYEYGHAAFNYAGHIYGATFFMATGFHGAHVIIGTIFLFVCLLRALKGHFTPKQH
                     FGFEAAAWYWHFVDVVWLFLFACIYVWGAGTPAAH"
     gene            complement(19915..20490)
                     /locus_tag="VF226_15075"
     CDS             complement(19915..20490)
                     /locus_tag="VF226_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX7075360.1"
                     /translation="MALICAAAALGMLGMAYAAVPLYTLFCQVTGYGGTTQRAQKPSD
                     TVLDRVVTIRFDANVAPGLGWTFHPVQQTKDVKIGENALAFYRAENTTDRPITGTATF
                     NVTPEIAGSYFNKIQCFCFTEQRLEPGESVDMPVSFFVDPAILEDETASHLTQITLSY
                     TFYPVEESKAAAVSQPVSPAAEKPSAGRKGS"
     gene            complement(20574..20744)
                     /locus_tag="VF226_15080"
     CDS             complement(20574..20744)
                     /locus_tag="VF226_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075361.1"
                     /translation="MAADDRNGEDFEAELERRRRQRYRSIAIAVALGLLVLLFYVATI
                     VRLGGNVFNRPI"
     gene            complement(20768..21700)
                     /locus_tag="VF226_15085"
     CDS             complement(20768..21700)
                     /locus_tag="VF226_15085"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX7075362.1"
                     /translation="MADKGTKAEARVIAASMGGTVGDFVALLKPGVMSLVVFTAFVGL
                     VLAPGELHPVLAGIALLAITVGAGASGALNMWYDADIDARMARTAARPIPRGHVTAEE
                     ALTFGVVLAVFSVMTLGLLVNWTAGALLALTIGFYIFIYTMWLKRRTPQNIVIGGAAG
                     AFPPMIGWAAVTGTVSIESVILFLIIFFWTPPHFWALALYRSRDYERVGVPMLPVVAG
                     GDETRRQILIYSVVLAIVAVIPPFIGLGGVVYLAASVALSVAFVALAIKVYRVREGRE
                     ADYAARQLFGFSLLYLFLLFAVLLAEHLVRPLLG"
     gene            complement(21851..23470)
                     /gene="ctaD"
                     /locus_tag="VF226_15090"
     CDS             complement(21851..23470)
                     /gene="ctaD"
                     /locus_tag="VF226_15090"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX7075363.1"
                     /translation="MASTASAAHIRDDHTPHGIKRWLFSTNHKDIGTMYLVFAMIAGV
                     IGGALSVGMRLELQEPGLQYFANPHAFNAMVTAHGIIMVFFVIMPAMIGGFGNWFVPL
                     HIGAPDMAFPRMNNISFWLLPVALGLFIISLFVEGPSGDMGSGTGWTLYPPLSTLGHS
                     GPSVDFGILSFHIAGASSILGAINFITTIFNMRAPGMTMHKMPLFVWAILVTAFLLLL
                     SLPVLAGAITMLLTDRNFGTTFFDAQGGGDPVLYQHLFWFFGHPEVYIMILPGFGMIS
                     HVVSTFSRKPVFGYLGMAYAMVAIGLVGFVVWAHHMYVSGMGVDAQAYFVFGTMVIAV
                     PTGVKVFSWIATMWGGSINFRAPMLWAIGFIILFTIGGVTGVMLANAGVDRALHDTYY
                     VVAHFHYVLSLGAVFAIFAGWYYWFPKMTGYMYSEFFAKLHFWLTFIGANILFFPQHF
                     LGLAGMPRRYADYPDAFAYWNYISSMGSYITAFGTSMFFVGMVVAFLRKERAAENPWG
                     VGATTLEWTLSSPPPFHSFETLPRIRAAEQH"
     gene            complement(23548..24555)
                     /gene="coxB"
                     /locus_tag="VF226_15095"
     CDS             complement(23548..24555)
                     /gene="coxB"
                     /locus_tag="VF226_15095"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7075364.1"
                     /translation="MGKSSLGIWALGSIAALAALGMATAAWAGIGQPSPGQLGMQGAV
                     TEVAEGIHAFHDLVNTLIIAIAVFVGILLLYVIFRFNEKSNPTPSRTTHHTMLELVWT
                     VIPILILVVIAVPSFKLLYLQYSYPKPDLTIKAIGNAWFWEHEYPDYDGLTVTSNILR
                     DEDLLRAELGDDEFNKRYGDLTGVERTRRLYNDAAPLWAKSGQPRQLAVDNEIAVPVN
                     KTVHVLVTANDVIHSWTIPSFGSKVDAVPGRVTATWFKVTRPGVYYGQCSELCGKDHA
                     SMPIAIRVVSEEAFNQWIEAAKARDWDRARGILRAATDQPDAAKVASAPPGTADRVEQ
                     N"
     gene            24962..25438
                     /locus_tag="VF226_15100"
     CDS             24962..25438
                     /locus_tag="VF226_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX7075365.1"
                     /translation="MGSFAAPAAPAIAQAPDGTVKAQHGDWQVVCKAPLPGAKNETCA
                     LVQSVTAEDRNNVGLTVYFQKFSNGTRVLRVFAPVGVLLPPGLGLKIDGKDVGHAPFL
                     RCHAFACYAQVVAEDGLVEQLKAGKTAIFIIFQTEEAGIGIPISLAGFSEALAELH"
     gene            25675..26916
                     /locus_tag="VF226_15105"
     CDS             25675..26916
                     /locus_tag="VF226_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075366.1"
                     /translation="MQSKRRTTTRKNVQHASVVVADLDGRRSQLLQSFHFSCRRKVAE
                     MASRAACFEELAETFPGLLFALATNYGTVESRRAAIDHLIAGSRLREAANALGLPWWM
                     RKLPPQAFSKRLESIPDDPAFAARVVNLLPAVPAGSAKWLDRVLLAYRACGPEFALWV
                     AKHDRFHAPLLTDPALIYVAAWAWFAGQPDTLGARLLRRPWTPAMSPRRAFDELTTWR
                     KRVRLALTLSALDRQPWLAEGSALGYDFVELREITDFIAESDAMDNCLDSFSEKLESG
                     TSYVFSIRHKGTPVADVEIGAHPIEPNLPTIVQLRAPRNRRASPQIWRAAYAWLGSQP
                     LRPAPPLVINSAARRRTWRELWSPYLSVLAPGDREQFEKLAKDLDKLRARRGRPARAR
                     RTSPAQQTVSPQTEEADVAMV"
     gene            complement(26973..>27695)
                     /locus_tag="VF226_15110"
     CDS             complement(26973..>27695)
                     /locus_tag="VF226_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX7075367.1"
                     /translation="LENVVVKAGTREPDFHDGSLTENTRAAYPLEFIRNSSPTGRAGH
                     PKNIIMLAADAFGVLPPIAKLTPSQAEYHFLSGYTAKVAGTEKGVGSEPQATFSTCFG
                     APFMPRHPTVYGNMLRELIAKHGVDCWLVNTGWTGGKYGVGHRMPIQVTRALLDAALS
                     GRLKGQAMRTDPTFGFAVPTSLPGIEEKILNPRETWTDPAAYDAQARALVDMFNKNFR
                     KFEDHVDADVKAAAPILRAAAE"
BASE COUNT         5105 a         8963 c         8812 g         4815 t
ORIGIN      
        1 accccgtcat cccgggcctt gtgcccggga tccaacaatc cgcaggcgaa gcgcagctgg
       61 cgaagtggat cccggcgaca agcgccggga tgactcggaa aagagcaacc gggcttccta
      121 atgacatctg cgtcaggcac atcttccaac ctcttcgccc cccgcggctc ggccgaggtg
      181 atcgagcgcg gtgacgtgtt ccagccaaag ttcggaccag atgacctgct gccggcgatc
      241 atcaccgatg cggaggcggg cgcggtgctc atgttcgctt ggatgaaccc ggaggcgctg
      301 gcgctgacga tcgagaccgg gctcgcgcac ttctggtcac gctcgcgaca gcggatctgg
      361 aagaagggcg aggagagcgg gaacctgctt cgagttgccg aaatcctgac ggactgcgac
      421 caggacgtgc tgtggctcaa gtgccacgtc gaaggcgacg gcaaggcctg ccatacgggc
      481 gcacggtcct gcttctaccg ggccttgtct ccgcaccgca cggaggatgg cgcggtccga
      541 ctcgtgcccg ccgcttcgac tgaaggttaa gcttagccga atttcccatc ttctgcgttg
      601 ttgataagcg gatcctccgg cgttgctgta gggtccagtt aatccagcgc gggttagcat
      661 gatcacgagc gactctaccc aaggtggcgt ttcaggcatc ctgtcccgcg ttgcatgctg
      721 gtgaaacgca acccatcccc gctcgggcgc aacgcggggt ggaaaggcgg agattgagcc
      781 atggcgaagg tgaagatcgg cctggcactc gggtgtggcg cggcgcgggg ctgggcgcac
      841 atcggcgtcc ttcgtgcgct gctcgaagcc ggtatcgtgc ccgacatcgt ggccggaacg
      901 tcgatcggcg cggtcgtcgg cgggtgctac gcggccgggc gactggacga agtcgaggag
      961 ttcgcatgca gcctcacgcg tcgtcgggtg ttcggctatc tcgacttcaa catgagcggc
     1021 tcgggactca tcaccggcca gcggctgcgg gaccggctga tcaaccatct cggtgacacg
     1081 accatcgaga gcctcaaccc ccagttcacc gcggtggcga cggagatcgg cacaggacac
     1141 gagatctggc tctcccgcgg ccggctggtc gatgcgattc gcgcctctta cgccctgccc
     1201 ggcatcttca agcctgtgaa tgttggcggg cggtggctgt tcgacggggc actggtgaac
     1261 ccggtgccgg tgtcggtgtg ccgggcgctg ggggcgcgtt acgtcatcgc cgtcaatctc
     1321 aacgccgaca tgtgcggccg cggtacggtg gtgccgttcc tcgaagcggg cacgcaggac
     1381 accctgctga tcgaggccaa cggcgtgccc aagaagaacg gccgcgccgc catgaagctg
     1441 ctgcgccgcc agttcttcgg ccccgacgag acggcgccgg gcatctccac ggtggtgatg
     1501 gacgccttca acatcgtgca ggaccgcatc gcccgctcgc ggctcgccgg cgatccgccc
     1561 gacttcatga tctcgccgcg gctcgccggc atcggcccct tcgacttcca ccgcgccgcc
     1621 gagctgattg aacgcggcac ggctgcggcc caccgtgagg tggaggacat cgcgcgcgag
     1681 atcgaggtgc gccgcatgtt cgagccgcgg gtggaggagg agcgggaggg ggctgtggtg
     1741 gaggcggggc gggagtagaa tgcgttggcc tacagagttc ctctagtcta atcgcggtag
     1801 agttacttcg gcgcgacaga aagcactcca aacaaaggag ccgccccgaa gggcggctct
     1861 cgaaggtcgg atgtcagcta tgatcagaac ttgataatcg caccggcatg cacgacgttg
     1921 gcgtcttcat ctgcgtcgaa gacgcccgca tcctcgacct cgtagttgcg ccagttgaga
     1981 tagagggcca tggcagccgc gtcgatgttc tgcacaatgc ctgcacccca gtatctccct
     2041 ttatcgtcaa ctggtgcagt atcgaagttg acctcgctat actccgcgaa cagtgtggtg
     2101 ttgccgtagc cgaagtagtt cttctcgaca ccagccatca cccagaagcc gtccttgtca
     2161 tcgagatcgt cgtcggagtc ggtggtggca taaccaccgg acaggaacag cccggtcggg
     2221 acatgcataa tagagcccga ccccagaatg gtctcttcgt ccaccgccgc tccgaccgtt
     2281 ggattatcct ccttcgcgcg gtagcccacg ccggcagcta cgcggacgcc attgaactca
     2341 cccgcatagc gaagcgccac ttcccagtag tcatcggcat cgtgaacatt atccccgcca
     2401 attaggtgcg ggaaaatgcc ttcgtccaac tcaccggtat cgtggctgta gccaaccgaa
     2461 agcaagaagc cagccagcga aggcgtggtg tagtagacgc cctgcttccg acctccgtcg
     2521 aagccattca cgaagatgtc ggtgaagtgg ctgtgcagcg agaattcagc ctcgtccata
     2581 ccgcccaccg taacgtacgg cgtggcgaga ttgatctcgt aaagaccgtc cgtcacctgc
     2641 gacgtctggc cgagattcac acgaccgaat gtctggctat tcaggtaaag gtagttgtgc
     2701 cggatcgtga acgaatcagc tgcagaaccg gtgccgaaat cgttgttgct gaggccaatt
     2761 tcgattacat agccagccgt gaggtcgggc cggatctgcg cctcaccttt gaagccaaag
     2821 cggctcatgt aggcgccgtt atcgcgaacg gtggccttac cttcgcgata gccgaactcg
     2881 tcatcatgcc agacaatggc ctggttcacc gcgccgtaca ccgttagcga caccacgcga
     2941 ttccccttcc gggccgccgt cgcttccagc tccgccacac gctcctcgag gtcggcgcag
     3001 cagttcccgc caaggtcggc ggccatggcg ctggtcgcaa acaaaccagc tgcggcagca
     3061 acagcaatgc tgctggccgt cttcatcagt cccctagtca ttcgataggc tcccctatgt
     3121 ttgtccggtg tgcagaaagc agcacgcgct gcccggcgac agcacacgcc cggtccgagt
     3181 tgcacgcacg cgcgtgagcg cacgccactc cgactggacg cgttacgcgc aacgcgactg
     3241 aactgaactc aacgctgggt actcaggtgc ggccttcagc ccgccgggct tgcttcctgc
     3301 taggcctgct cgggaccgat gtggaccctc actggcccat actcgacctc ctaaagaggt
     3361 ccgccgcacg actcgatact ggacatgatc gttagaggag gacgggtcga ggggaaaggg
     3421 taagtcgaca gaaatccgct caatccgacg gaaccgtggc cgatatgtca ccagttgaag
     3481 gtgggcaaca gtgttgttgc tggaatgttc cggatttctg gagcggttca gatcggatcg
     3541 atgttaaggc cggagtcggc tgtaccattc cgagggcaca aaaagcaaga aagccgcccg
     3601 ttggggcggc tttcggttcg gtcagtctgc ttaggctggt ggatcagaag cggatgatgc
     3661 cgccggtgaa cactgcctga tagtcctcga catcgaggtt cagacgggtg ccgaaggggc
     3721 ttgctccgac attgactacg gcatcgaagt cacccgagta gttgcgatag cccgcgtaaa
     3781 ggtccattgc cgctgcatcg atggtttgaa cgataccaaa gccccaaacg gttacctgag
     3841 aactcctgat tgccgccccg aaggtatcaa agttagctcc cagaggcaga gtacccgcca
     3901 ggaagccgag atcgtttacg ttgccgccag ccagaccacc acctgtgtca gcctggaagt
     3961 actcgccgta gatcgtcgta ttgcccaagc cgaagaagtt cttctcgata ccagcttgga
     4021 cgtaccagaa ctcgtcgcga tcatcgacgt ccgggagaag caaattgcct tccaaagcgc
     4081 gcaatgcgaa caattcccgg cgattctcat ctttgaggaa gccgtaagcg cctgcgatgt
     4141 aaagtccggt cggtacatgc atcgcactgg ctgacatgcc gacggcccag cagtcgacgt
     4201 cggaatctcc cgtcacaccg ataccgaaca ccggatctac gaagtcgaga tcggcgcaac
     4261 cgcgatcatt attgctgcca gtacccgtga cagcaaagcc cgttatccca aagatatccg
     4321 ttggcagaac cgcggtcgca tcgccgtccg tccactgttg ataaccgata cccgcggcga
     4381 cccgaatacc gttccactcg ttcgcgtagc gcagagccgc atcccaaaag tcatcctcac
     4441 cccacgaggc cgagaatgtg aagcccgaga aggtgggcga aacataacgg acgagattgc
     4501 gccggtcgcc atcgccgacg ttcgcattgg cttgggacag tatgtcattc cactgcagac
     4561 cggcgaaggg cgccaccgcc aacaatgtcc cgttgcttaa gcgcaggaac atgccgccgc
     4621 cccagttgtt cacgtccggc gcaccgatct gagcagcgtt cgacaggttg atttcagtta
     4681 tgccgtccgt cgccatgctg gtgtggccca cccagacacg accaagccgg ttcgactcga
     4741 tccaccaggc actatgcctg aggtccaaag atgaagtgcc cccgacgagg tcatcggcaa
     4801 actggttcac attggcgctg ttcgcgatgc gaacgccgat ctcgagcaag taccccgcct
     4861 tccattccgg gctgatcgtg gcgtcgccgc ggaagccgaa gcgggtacga gagttctcgt
     4921 tcgtcaccac gtaggcgttg gtttcctcgc cgtcatccca ccacaggatg ccttcgttca
     4981 cctggccgta gacggttaga gacacgcggc gattgccctt gcgcgctgcc gtcgcctcga
     5041 gctcggcaac acgctcctcg aggtcggcgc agcaatttcc gccgagatcg gccgccatgg
     5101 cactggtcgc gatgacgctg gccgcggcgg cgatggcgaa gcgactggtc gtcttaatca
     5161 gtcccccaat catttccatc tccacttggt gttgttccgt tggcgcagaa agcagcacgc
     5221 gctgcccggc gacagctcac gcgcctagtc cgaggtgcac gcacgcgcgt gtgcgcacgc
     5281 cactccgact ggacgcggta ctcgggagac gccactgaac tcgacgctga actcgtagtg
     5341 cggcttacca gcccgccggg cttgctttct gcagagcggc caggctgcca tcggatgccg
     5401 ggccagctct ggtggaccct cgaagaggct ccgccgcacg acactcaaca cagcggccgc
     5461 accggttgca tgtcgctgac gaacacccgt tatggttgtt cgcattcctg ctttagtgca
     5521 gttgcaggtt tcgagcaaac tcgcgtttag gttgttggcg cgattgatct gaattcgttg
     5581 cgcttttgtc acgaagtgcg catcagcaac ggcaatttcg gccgctcacg ggttagtcag
     5641 gcagggagga gatgtggatt gcggggggcc gaagatgtga atcgggtgtt ggacggcgcc
     5701 tctgattcga gtcgccgttt ctgatgcgac caaactggtt cctgatttga tcgccaaccg
     5761 tatcggccgc ggttcacccc gcgagcaggt aggtgcggag cgcactggcc tccatctcta
     5821 cctcggcgat gtgatgcttg acgacgtcgc cgatggagac gatgccgacg agcgcatcgt
     5881 cctcgagcac gggtatgtgg cgcatgcggc cggccgtcat ggtcgccatg agctggtcca
     5941 gtgtgtcagc ctcgctgcag accaccacct gccgggtcat gaattctgac acgggctgct
     6001 cgaggcagtc tcggccccgc tcggcaatgg cgcggatgat gtcgcgctcc gagatgatgc
     6061 cgacgaccgt accgctcgca cccaggacga cgagcgagcc gatgcgcttc gccgacatct
     6121 tctcggcggc ggcttgcagc gtcgtctctg gccgcgcggt agcgacggcc cgccccttgc
     6181 tcttgagtat ggcagccacg ttcatggacg cgacctcccg tccaggcggc cggcagaccc
     6241 gtactgacac gccgagggtc gcggccggtc tggccgacaa cgtgtccgta gccgggccgt
     6301 tccaaccgcg gctcagttac ccctcgcata taggggagat caactcccgg tagttgatcg
     6361 tgcgctatct cgcccgctgg cgcaagccgg tcgcttcgct accgcagcgg agggtcagcc
     6421 tcttccagcg tcggctcagc cgcgcgatcg aatgcaccga agatgagcag cccggcgaag
     6481 aaaccgccga ggtgagcttc ccaggcgatc ccgcccgttt cggcgaggtc accgaatccg
     6541 atcgcgaagg cgaagttcag gatcacccag attgcgatgg tgatcagcac ccgccggtcg
     6601 tgcagcatct gcccgagatt catcctgggg acctcgcggg cgccttccgg aatgacagcg
     6661 gcccccccgc gatccatggc attgaacaga aaccgcatca ggccgcccat catcccggat
     6721 atggcgcccg aagctccgac catcggcacc ggcaatcctg gattgatcgc cagaaacgtg
     6781 agcgcccccg cgatgccaca gatcgcgctg aacaagagaa atcgcagact gccgatgcgc
     6841 cgcaccagcg gcgtgcccaa tgcgagcagc caggcactgt tgatcagcag gtgcagcggg
     6901 tcgccgtgca cgaacatgtg ggtgatgaac gaggtgacgg cgctgatctc gccgccaggc
     6961 agcacgaagg ggctatcccc gtaacgggcc ggaatgaacc ccagcgtcag aaccagatac
     7021 gcgtcgtcaa tgtccgacag aaatccgcgc gcgacatgga gcagggcgaa cgcggcgagc
     7081 agccccaaaa tgacgtacgg cgcgttgaaa atgggctctc ggcgcaccaa ccggtccttt
     7141 cagcgagaca tttcatctag agggccttca ccatgccggc aagcgcccgg cgctcagaag
     7201 ccgcaggccc cgagctggca tggaacctgc tcaggaggcc gggaagaagc tgcacatgtc
     7261 ccagatcgca actcggggcg gaactggttc tatcgagtat ttggaggggg atcgatggct
     7321 tggggagacg ccggaaggga gcgcgccgcc aacatggctg aaaggccggg cgctcaggtg
     7381 gatcgtatga agaacgacac gagccgagtc cttttccatt actggaatgc gatccgcgga
     7441 tcgcgccggg cgccgcgccg cttcgagatc gagccggccc gcatctcccg cattttgccc
     7501 gaggcgttca ttctggaaca acgggacgtg gacacatacc ggttccgaat tgccggcacc
     7561 cgcctttgtg aactgttcgg gatggagctg cgtggtcaca atttcctgga actttgggag
     7621 gatggggacc gattcccggt gaagcgccgg cttgcatgcg tcgcggaaga gggcgcagtg
     7681 ggactgttcg aatttgaggc atctgtgtca gtacgggaat cggcgagctt cgaggtgctg
     7741 atcgtgccgc tcatccagaa ggatgccgcc atcgaccggt tcctcgggac catgagcccc
     7801 atcagcgagc cgctttggcg cggggtggaa cgcctggagc gatgtcacct gctgcggcac
     7861 gagttgatct ggcccgaacc tgcgtcgggc agaattgtcc gtgcacccct gccggaagtt
     7921 cgtgacgccc gcctcgtgcg cgtcgcccat cgcagcttcc gcgtttacga aggcgggctc
     7981 tcgcgcgagt ccggcaagcg atagcgcacg cgtcctctta ccccaaaaac agcagaaaat
     8041 ggacttccgg cacagttcgt cgcgccggga ttacgtttcc ttaacggctc gcacggatag
     8101 tcaaggctta cgcaaggaca atccgggcca tcggtcaggt acacggctgc agtacggctg
     8161 cagacgggct gatgtatcag tgcgagctgc tcctcatgac ttggacgccg atcaccgcaa
     8221 cagttcccga cggagcggac ggccggcccg cggaagcacc tctggcctcc ccgcgcgagc
     8281 ggcggctcca taagcgctac aagatcgtcg ttccgggccg attcatgcgg ctcaacaagg
     8341 aagagcatcc gtgcaggctg cgggacatct cctgcagcgg cgcggcgatc acctcacctg
     8401 tcagcctcga gatcggcgag cgcatcgtcg tctatttcga gcatatcggc gggctcgacg
     8461 gcatcgtcgt gcgcacgttc gaaggcggct tcgccatcga gctctgcgtg acgcagcaca
     8521 aaagagagaa actcgccgac cagctcgcgc gactggcgaa ccgcgacggc aaacccgcac
     8581 cccgccggca cgagcgattc accatcaaca acaccacgac ccttcgtctc gacgaagaca
     8641 tctccattca ggtgcgcgtg ctcgatgtgt cgatttccgg cgcctccgtc gagacggagg
     8701 cgcggccggc tctcggcagc gaagtcgtcc tcggcaagct ccgggccaag gtggtccggc
     8761 atcatatgga ggggctcggt cttgaattca tggacataca aaaccccgat gctctccgtc
     8821 gctccatccg ctgacattat ccccagacgg atcagtatcc gcggctgttc gagcacggcg
     8881 caacacgatt tcgtcgcaaa ccagcggcag gttaacgggc aaagtgtagc aagcgatctg
     8941 ccggttgtag agggcgttgc agatcaattg cgagttgtgg gtggggttgc gtgacatgcg
     9001 agtaaaagct ctcgtgctca tagtcgtgtc cgggttgctt catgcgggca gtgccgccgc
     9061 cgacaactca aaaataaggg cagccgagca ctcatctctc tacatgcgcg tcttcggaca
     9121 ggcgattccg ccgttcggat tcatccagtt ctgcgaggct tggcccgacg agtgcaaagc
     9181 gggtcactgg cgcgacaggc gcttccaggc cacgccggaa cggctgagcg agctggacga
     9241 ggtcaaccgc tacgtgaacc gccgaatccg tccgactacg gaccaggagc tgttcggcgt
     9301 caccgagcgt tgggtcatcc ccaccgacca aggcgattgt gaggactacg ctcttttgaa
     9361 gcgcaagatc ctcatcgagc gcggatggcc gccgagctcg ctgctgatga ccgtcgtgcg
     9421 cgacgagctg ggcgaggggc acgccgtgct cacggcgcgg acagcccagg gcgacttcat
     9481 cctcgacaac aaggtcgacg aggtgatgct ctggtatgtg aagccctacg agtacatcat
     9541 gcgccagtcg ttcttggatc cgaaggtctg ggtgtcgctc aacccgcagg cctactcgcc
     9601 ggccgccatt gccgttgtgc gcaatcagga ctgagctccc ggataagggc cggagctcag
     9661 atgacccgcg tgcggggatt gctggcagtg agcccataca cgtgagccca gccgctggcg
     9721 cctcgtagcc agcacccgct catggcaagc cgccgacgct gcgtcgttgc ccaaagcgcg
     9781 tcgcgccggc gtgccgccta ttggttctcc cgcagttgat ttaccgtgcc ggggttctga
     9841 cagaaccgaa tgcgggtgca gccgccgtga tggcactgtt gccccgagct tccccccgcc
     9901 agtctcgaag caagcgacat ctactcgagg tgtcgatgcc gattcctgag ttgtcgggag
     9961 gaacgagacg tcactccagc gtggcggctt cctcgtgagg aaatcgcaac acagctacag
    10021 atagcgcccc cttcaccgat ttccggttgg accgcccagt gccgggcaga cctgccctca
    10081 gtgcgacgac cccttggcct cgacgcgcgg ccggacgggc tctgctggcc cactcgccgc
    10141 accattgccg ctcttgtcgg ccggccgggc gttggacgca tccgcaatcg gcattcccgc
    10201 gagcttggcc aggctttctg cgatcgccgg cggtagggac ggaaactgcg gcgcgttctg
    10261 ccgagccccc ggcggaggtg ccggcggagg ggtgcgcccg gcagcggctc cgttgggtgg
    10321 tggaggcgcc gtgtgtgccg gctgtcgcgg tggcgccgta accgaagatg gtgcagcagg
    10381 cggtggcggc ccaggctgtc gagcgacagg tggagcggtg gggcgagccg gtggccctcc
    10441 cttccgcatc gagggtggcg gcggcgccgt cgtggggcct ggccgtggct gttgcggaga
    10501 agcggccacg gctggctggc gcggatgcgg cggcgcggtc ttcgggcctg gcgtgagctt
    10561 cggcggcacc ggtggcgggc cgggaggctg ctttgcctga ctcggtcgcg ggggtggcgg
    10621 cgcagacctc accggctgct gcgctccatc ctgcggcggt ggtggcggtt gggtctgctg
    10681 ccgaaccggc ggcggctggg tccgcggcac gccacgagac ggaggttgcg tgcgtgcact
    10741 gctttcgccc tgggcgccag ccggtgctcc ggccggtggt ggggcagcgt gcgaaccttt
    10801 cggctgaagt ggcaagggcg gaacggcgac gggcgagcca gtcttctgtt tcgacgtcga
    10861 atcgcctgcg gacggccccg ccgccggatc cgcaccgtcg gacttcgcgc cgccggctcg
    10921 ctttcccgaa gtccgcgcaa ggagctgttt ggccgcttgc agcccgcggc gccccgcctc
    10981 cggcttgccc ttctccttcc ggctcttggt tggctcggcc gggcggtcaa gagtgctgtc
    11041 cggagctgtc acgttcgcct tctgcgacgc ctctgcaggg gtgagcgcag gcgcctcggc
    11101 cgttgcgggc gctggcgcgc tcacctccgt gcgcttcttc ctcctccggc tcaaggaccg
    11161 cggcctgaac agcccgctgc gctgcaactt gcgctcggac ttgcggatcg tgcgcacgag
    11221 ttgcagcgcc tcgcggtcgc tcatcgcctc gccgtagtaa tagccctgcc cgtactcgca
    11281 tccgagcccg cgcaggaaat tggcgtcctc cgccacctcg atgccttcgc cgacaacctt
    11341 cttcccgagc tcgtgcgcca gcgccacgat ggagcgcacg atcgcggcgc ccgggccgtt
    11401 accgttgatc gcttgcacga gcccctggtc gatcttgatg gtgtcgaacg ggaaccgctg
    11461 gagataggcc agcgaggaat agccggtgcc gaaatcgtcg atcgagaggc ccgcgccggc
    11521 actgcgcagc agctccagca cgtgcaccgc ctgctccgga ttttccatcg ccaccgactc
    11581 ggtgatctcg agcctgagcg cgcgcggcgg caccagggcg cggcccagga tgtggcgaat
    11641 ttcctggatg agatcctgcc ggaagagctg ccggctcgac acgttcacgc tgacgaagac
    11701 gggattgtcc ccgcgtggca gctccttttg ccacttggcc gcctcctcgg ccgcgcgcgt
    11761 gaggacataa gagccgagct tgacgatgag atcgctcttt tcggcgatgg gcacgaactc
    11821 cgccgggctg agccgtccga gcttcgggtg gtcccagcgc acaagcgcct cgaagccggc
    11881 cagttcttcc gtcggaagat agatgatcgg ctgatactcg atgtggatct gattgcgctc
    11941 gagcgcgcgc ctcagatcac cttcgagaac cacgcgatcg tcaggctccc tgcgcttgtc
    12001 gggcatgaac atctcgatcc ggtcggcgcc gctgcgcttg gcgcggtaca tggcgatctc
    12061 ggcctgcttg agcagctcct cggcgtcgag gtcgccatcc atgacggcga tgccggtcga
    12121 tgtggtcagc gagatctcct ggccggcgat tttgatgggc gagcgcagac tgcgccgaac
    12181 ccgctccgca agcagcgcaa gctcgcgcgg ctcatgatgc gagacgagca gcatcgcgaa
    12241 ttggtcgcca ccgacccggg ccagcgtgtc ctgcggaccg acgtggcgca tcagccggcg
    12301 tgccactgtc agcaggatgc tgtcgccgac gatcaacccc agagaggcgt tgacgttctt
    12361 gaaccggtcg atgtcgatga agaaaacggt cgggcgaagc tgctgctcga ccttggcgcg
    12421 catgatggcg acgccgagcc ggtccagaaa cagcgccttg ttgggcaggc cggtcaggct
    12481 gcagtggacg gcatcgtgca tcagccgttc ctgcgcccgc ttcgcctcgg tgatgtcgcg
    12541 catcagaccg acacagcgca gtgcccgccg gtcggaatgt gggacgcttg ccgcctcgat
    12601 ctcgaaccag cgataggagt tgtcggtgtg gcgcatcctg agctcgatgt gaatcggccc
    12661 gccgttgcgc tcctgcacac cccacagcac cagccggaac cgctcccggt cggccggatg
    12721 cagatgcttg gcgaagtcgt cgaccttggt cgacagctcg cctgccgtca ggccgagaga
    12781 ggcctcgatc acggggctca ccttgatctc gtcgcgccgg gccagccact cccagaccgc
    12841 cgcccccgat ccgtcgatcg ccatcgatcg aagctgcatc tcgctcggtg cagcgccata
    12901 gaccggctcg aaggaacgga atgcgaactg cgtgacggtg aagcccatca acacgacgat
    12961 gaggaccagt ccagcagcca gcgccgagac aacgatatcg cccgagagct gccccgtaag
    13021 ggttacagcg gccgcaaaga tccacaccag gaacaggagc caggtcggga tcagggcgag
    13081 tgcgcggtcc tggccgcgaa tggcgaggta gagcgtgagc agcgccccaa ggctgccgat
    13141 cacggcgaat gaggcgcgcg cgaacgtggc cgccagccgc gggtcgatca cggcgacgaa
    13201 aacgagtgcg agctgggcag cgatccagac gctcgcgacc atgcggatga acccgtgcca
    13261 ggagctcagc cgcaggaaca cgctgacgaa gatgacaatg ctcgccgcga tggccgactc
    13321 ggtggcggcc cggtagacgg cattatcttc cgccttcagg tggaaaagct tgtgccagaa
    13381 gccgaagtcc acgcacaggt agccgagcac gcaccacgcg accagtgcgg cagccgggaa
    13441 gatcgccttt tgattggcgg caaaaacggc cgtgagaaag atcgcgagca ggcccgtgat
    13501 ccccagcatg atgccgttga agagctgtct gtcgcgcgca ttcagctcgt actccagcga
    13561 cttccagagg aagacacggg gaatgcgctc tgaggccaac tcggcggcgt aggtgatcgt
    13621 ctgccccggc tccaacgtaa tgcggaagat gtcggcccgg tcgctcctga cgcgatccgg
    13681 cagatacccg agcgacggtg tcacggcctc gatgcggcgg gtgtcgagat ccggccagat
    13741 cacggacgag ccgatcaccg tgtaccgctc ggccgtcagc cagcgctcga tcggtttgtc
    13801 ggtcgggttg cgcagtgcaa agacgagcca gttcgggttg gtgccaggcg tgctcgcccg
    13861 gatcgacatg cggccgctga cgccatccgg gcccgaagcc gtgtcgacct ggagcgtgtc
    13921 gccctggccg gtgtggatct caccctggcc ggtgatctcg atgcgatcct ggtcgggccg
    13981 gacctctatc ggctcgagag cgcgcgccgg ctcggccgca gtggtgagga gcagcagccc
    14041 gaacaccagc agcatcaggc ccttcaggta ttcccccacc ccccgcgatg gtgcggcaga
    14101 gcaacatgga tcgctatcgc tcattccgta tcccggaccc gccgaagtcg tcccgcgggt
    14161 cgtcagccaa ggttgcgtag agcagatgat cttgccagac accattgatc ttgagatagc
    14221 gccgcgccag cccctcgcgc tggaaccggt tgcgctccag cacgcggatc gaggccagat
    14281 tgttcggcat gcaggccgct tccacccggt gcaacttgag ctcgtcgaaa gcgaaccgga
    14341 cggcgccggc aagcgcccgg gtcatgtacc cgcgcccggc atagggcttg cctacccagt
    14401 agccgactgc ggcggcctga gtgacgcctc ggcgcacgtt gctcagagta agtccgccga
    14461 gcagggtgtc gtcggcttcc tgaaacagga agaaggcata ccccatgtcg tcgcgcagct
    14521 cgcgctggta gtggcgaatc cgccggcgga aggcatcgcg ggacagctcg tcgcgcgtcc
    14581 actgcggctc ccatggaacg aggtggtcac ggctcttcgc ccgcagttcg gcccaggcgg
    14641 cgtagtcgcc catgtgcggc ggccgaagat agatcgaccc agcacgcacg agaggaagcc
    14701 attcactgcc gaggccggat ctcaggaaag ccatggcgtt gctcctcttg ggctagccgg
    14761 cgaggcgacg ctcggcccgc tgcgcataat cggcagaacg tctgccggcc cccacgacgg
    14821 cgattgacgg tagagctgat cgcgcaacgc gttcggcgat ggcccggatc cgttcgggcg
    14881 tgacatcctc gatccgttgg acaagctcgg ccggtgggat caggcggtcg aagaacatga
    14941 gctgccgcgc catctgctcg gcccgcgccg aggagctttc gagccccgtc agcagcccgg
    15001 ccttgagctg agccttggca cgcgccactt ccgcatcgga cgggggcgtg gcggcgatgt
    15061 tgaacagctc cgtcccgatc acgtcgatga gctcccccat ttgctccgga cctgtcgccg
    15121 catggatgtc gaacatcccg caatcggcca tgccccatgc cgtcgagtag atcgcgtagc
    15181 aaagcccccg attttctcgc gccgcctgga agagccggga cgacatccct ccgccgaaga
    15241 gcccggaaaa gacctgcgcg gcatagaact cctggtcgcg ataggatggc gcttcgaacc
    15301 cgaggagcag atggctctgc tcgaagagct tccgcgaggt gcgctgccct ccaacgtatc
    15361 gcgccaccga accatcttgt gattcgccgc catacaggcc tccaaatagg gcttcagcgt
    15421 ggcggacgag cgtgccgtgg tcaatcgctc cggctgcgga aatcaccatt tgtggtgcaa
    15481 gatagtgggt gcgcaggaag ttcctgaggt cctcggaggt cagcctggtg acgctttcgc
    15541 gcgtgccgat gatcggcctg ccgacggcct gcccggggta agccgcatcc tgaacgagat
    15601 cgtaggcgat ctcgtcgggg ctgtcctgaa tgctggcgat ctcctgcagg atcacctccc
    15661 gctcgcgctc tagctcggcc tccgcataag tggagttctg caggatgtcc gccaggatct
    15721 cgagcgcgat ggcctcgtca cccttgagca cgcgggcgta gtaggccgtc atttcgagac
    15781 tggtcgccgc gttgagctcg ccgccgacct gctcgatctc ttcggcaatc tgacgggccg
    15841 tgcgccgccg cgtacccttg aacgccatgt gctccaagag gtgggagatg ccgtgctctt
    15901 cctccctctc gtgacgcgca ccggttgcca cccatactcc gagcgagatc gtttcgagat
    15961 ggggcatctg atgggacacg accctgagcc cattgcggag cctcgtgacg gcagtggtca
    16021 tggttgctgc tccacacccg cgcccacccg ctcctccacg aaacgctcca cagcagccag
    16081 atcatttggc aggcgaactg agcgctcctc atcggtcatc aaggatgcaa gccgtggcgg
    16141 caggcccggt cgctcgccca gaatggtctc catggtgtcc gggaatttcg ccggatgcgc
    16201 ggttgcgagc acgacctgcg gcgccccacg ggagggcagg catcgctgcg ccgcgatgag
    16261 gccgcaggcc gtgtgcggct cggccgtgta gccgctcgac tgccgcaccc ggcggatggt
    16321 ctcggcgacc tcggtctccg aggccgaagc ggcggaaaag tcttgcgcga taagcctcgg
    16381 gcctcccctc acctcgaagc ggcccgactg cgccagagcc gtcatctggt tccggaccag
    16441 cgctgcatcg cggccctggg cttcgaacag gtagcgctcg aagttcgagg acacctcgat
    16501 gtccatggac ggcgaggtgg tcgcggtcac gccccgcttc tcgtagatgc cggtggccac
    16561 cgtgcgcggc aggatgtcgt tgacgttgga gccgatgacc agccgctcga tcggaaggcc
    16621 catctggcgg gcgacgtagc cggcgaagat gtcgccgaag ttgcccgtcg gcacagcgaa
    16681 cgagacagac cgatgcggtg ccccgagcgc cacggcagcg gcgaagtaat acgtcacctg
    16741 ggcgacgatg cgcgcccagt tgatcgagtt cacgcccgaa agccgcatgc ggtcgcggaa
    16801 ggcatggtcg ttgaacatgc ctttcacgag cgcctggcag tcgtcgaacg ttccctcgat
    16861 ggcgacggca tgcacattgg gctcgcgcgg cgtcgtcatc atgcgccgct ggacatcaga
    16921 gacccggccg gccgggtaga ggatcaccac gtccacggct ttcgagccgc ggaacgcctc
    16981 gatggccgcg ccgccggtgt cgccggaggt ggcgccgacg atggtggccc gctgcccgcc
    17041 ccgcttcagc acgtgatcca tgaggcgcga caggagctgc atggcgacgt ccttgaacgc
    17101 cagtgtcggt ccgtgaaaca gctccaggac ccaaaggttg ctgtcgacct gcaccaacgg
    17161 cgtcacggcc gggtggccga atgttgcata ggccgacttc gccatggcca acagctcgtc
    17221 gcgcgagagc gcgccgcccg tgaaggggtg gatcacctcg accgcgacct ctgagaacgg
    17281 ccggcccgcg agcgaggcaa tggctccgtt gtcgagcttc ggccaggtct cgggcacgta
    17341 gagcccgcca tcgcgggcta gaccggcaag cagaacgtct tcgaagccga gctcgggcgc
    17401 caagcccctg gttgaaatat atttcacgga acgtcagcct tccttatgcg ggcgcccggg
    17461 gggcgcggac gtaacctaga gcgccccatt gacccgcaca agcggccctc atcggggcga
    17521 ccgtgacggc gggcgtggat cgaggacccg cgactcgctt ccccgcaacc ggctgatcac
    17581 aaaggcgata tagacagcca agagggtcag cgcgagcccc caccaggtca gggcatactc
    17641 cagatggcgg ttcggcagct cgagacggct cggcctgggc gccggcaagc cgcccggcgg
    17701 ggtcgggccg atctggtcga cgtaaaagtc gagcccgctg gggcccgagc cttgccatcg
    17761 cgccagccgg gccccttcca caccggagcg ggtgaggaat tctctcgggt tgcgcagaaa
    17821 ccatatgttg gccgccggat tgctctcacc ggtgaacatg ccagcctgct cggccgtgcg
    17881 gagctggccg atcagttccg tctcgccttg gatctgtcct tcgggccggg tttgcgggtc
    17941 cttgcgggcc tccggcacga agccgcgatt gacgtagatc cggccattcc ctccagcgag
    18001 ctcgaacggc gtaaagaccc agtagcccgc gccgccaggg ctggcgccgg ccggtgcagt
    18061 cgtgaacacg tgccgctcgt cggggttgtc gaacttgccg acgagacgga cggtcatgaa
    18121 ctcgcaggac cctgcaaggc cgacctcgtg caccggggag caggtgagcg tgctccaccg
    18181 ctcgggcggc agcggctcgg ccgaggcccg cgcctccatg gtggcgatca gctcttcctt
    18241 ccaggccttg cgctgccatt gccagttgcc gagcccgatg agcaggacga gtgcggcgag
    18301 cgtcgcaacg gtcagggcga caaggccacc tcctctcaac ctctgcgcca tcagcgtccg
    18361 tcctggtcga aacggccctc ttccgccctg ttgcggtact ggagggcgat gagcgtcccc
    18421 ttcatcggcc ggagcagacc gtagccaagc agagcggcaa tgggaaacca gaggaccacg
    18481 tgcacccaga gcggcggcga cagcttgaac tcgacgatga gggcggctcc cataatcagg
    18541 aacccgagta tgatgatgac gaacacggcc ggcccgtcgc cgctgtcggc aaagccattg
    18601 ctgaggccgc aagtctcaca gcgctcgcgc aacttcagag tcagcggccc atcgaacagt
    18661 tgcccctctc cgcaacgcgg gcaacgtccc agcaggcccg cccgcagcgc agttccaagc
    18721 tgatcatcct gagccatgcg ggagatgtgc tgcactcgcc ccatccgatg caacgtgggc
    18781 tttcgccaca gagcagctcg agctcgaaaa acggaacggc ggggccgcaa ctgccccgcc
    18841 gtgacctgca tcgcaccgat ggtttgttct cagtgcgcag ccggtgtccc ggcaccccag
    18901 acgtagatgc aggcaaaaag gaacagccac acgacgtcga cgaagtgcca gtaccatgcg
    18961 gctgcctcga agccgaagtg ctgcttgggc gtgaagtggc ccttcagcgc ccgcagcaga
    19021 cagacgaaga ggaagatcgt gccgatgatg acgtgcgcac cgtggaagcc ggtggccatg
    19081 aagaacgtcg cgccgtagat gtgcccggcg tagttgaacg ccgcgtggcc atactcgtag
    19141 gcctgcagca gcgtgaacat gaggccaagg caaaccgtca gcgtcaggcc ccagacgaga
    19201 cccttgcggt cgttctggag caacgcgtga tgggcccacg ttaccgtcgt gccggacagc
    19261 agcaggatca gcgtgttgac gagcggcagg ccccacggat cgaaggtgct ttggaacgaa
    19321 tccgaaggaa tgggcggcca ctgcccgccg gtgagctcgt tgcgttcgac caaaccgatg
    19381 atcgaagtgt cagaaggtgc gatcgagccg tccggcatgg tgatgggcag ggggctaacg
    19441 cctcccggga acagggctgc gtcgaaatag gcccagaacc aagccaggaa gaacatcacc
    19501 tctgaggtga tgaacaggat cattccatag cgcaggtgaa gttgtacgac cggggtatgg
    19561 tcgcccttgt gggcctcccg gaccacgtcg cgccaccaca tgaacgcaac ggcgaccagc
    19621 gcggcggcgc caatgaagaa gaccgtcgag ccctgtacgc cgaacaaacc ggagccgccg
    19681 cctatcgacc gcatccaaat gatcaagccg acggccagga cgaacgcgct tagggaggcc
    19741 agaagtggcc acggactcgg gtcgacaaga tgataatcgt gatgcttggc gtggctatcg
    19801 gccatgagtc agctccgtcg tccttgtgca ttccatccca gcatgcagcg ccggatggtc
    19861 cgcgcccccg gtgttagcga aatcctcgac agctcggcgt cacaagccgt catgtcacga
    19921 ccccttcctg cctgcacttg gcttttccgc cgcaggggaa accggctgcg agacagccgc
    19981 agctttcgac tcctcgacag gatagaaagt gtaagagagc gtgatctgcg tgagatgact
    20041 cgccgtctcg tcctcgagga tggccggatc gacgaagaag ctcaccggca tgtcgacgga
    20101 ctcgcccggc tcgagccttt gctccgtaaa gcagaagcac tggatcttgt tgaagtagct
    20161 gcccgcgatc tccggcgtca cgttgaaggt cgccgttccc gtgatcggac ggtcggtcgt
    20221 gttctccgcg cgataaaacg ccagcgcgtt ctcgccgatc ttcacgtcct tcgtctgctg
    20281 gacagggtgg aatgtccagc cgagtcccgg tgcaacattg gcgtcgaaac ggatagtgac
    20341 gacccggtcg agaactgtgt cggacggctt ttgagcgcgc tgggtcgtac cgccgtagcc
    20401 ggtcacctgg caaaagagcg tgtagagcgg cacagcagcg taagccatcc ccagcatgcc
    20461 caaggcagcc gccgcgcaga tgagcgcaat gcgcgcgtgg cgcgccccac cacccacctt
    20521 ctcgactgac cccgtcacga caaataaccc tttctcacac cagccctgcc cgatcatatc
    20581 gggcgattga agacgttgcc gccgaggcgc acgatcgtag ccacgtagaa cagcaggacg
    20641 agcagcccga gcgccacggc aatggctatc gagcgatagc gctgcctgcg ccgccgctcc
    20701 agttcagcct cgaagtcctc tccattccga tcatccgcag ccacacggcc actccccaac
    20761 gtcatactca tcccagcagc ggccgcacga ggtgctcggc cagaagcacg gcgaacagca
    20821 ggaacagata gagcagcgaa aagccgaaca gctgtctggc cgcgtagtcc gcctcgcgcc
    20881 cttcacgtac gcgatacacc ttgatcgcca gcgccacgaa cgcaacgctc agcgccaccg
    20941 acgcggcgag ataaacaacg ccaccaagcc cgataaacgg tggaatcacc gcgacgatcg
    21001 cgagcacgac gctgtagatg aggatctgcc gcctggtctc atcgcctccg gctaccacag
    21061 gaagcatcgg cacgccgacc cgctcgtaat cccgcgagcg atagagcgcg agcgcccaga
    21121 agtgcggcgg ggtccagaag aaaatgatga ggaacagtat gacgctttcg atgctgaccg
    21181 tgccggtcac ggctgcccac ccgatcatgg gcgggaatgc gccggcagct cctccgatga
    21241 cgatgttctg cggcgtccgc cgcttgagcc acatcgtgta aatgaaaata tagaagccga
    21301 tcgtcagcgc gagcagcgca ccggccgtcc agttcaccag caggcccagc gtcatcaccg
    21361 agaacaccgc caggacgacc ccgaaggtca aagcctcctc tgcggtaacg tgcccgcgtg
    21421 ggatcggcct tgccgccgtc cgcgccatgc gcgcgtcgat atcggcgtcg taccacatgt
    21481 tgagcgcgcc ggatgccccg gccccgaccg taatcgccag aagggcgata ccggccagta
    21541 cggggtgaag ctcgcccggc gcaaggacca ggccgacgaa cgccgtgaag acgacgagcg
    21601 acatcacgcc cggtttcagc agggcgacga aatctccgac cgttcccccc atcgaggcgg
    21661 ctattacgcg cgcttcagct ttggtgcctt tgtccgccat ttcggctcag cctgccggct
    21721 tatccctgtt ccagtcgacg gcgcaccgcg gcgccgccta ttctgttatc ccgcctgttc
    21781 caggcgggcc gccgatcgaa gcgcccgcct catggtgcga ctgggaggcc gaagcctccc
    21841 atcgctcttg tcagtgctgc tccgccgccc tgatccgagg cagtgtctcg aacgaatgga
    21901 acggcggcgg cgaggacagc gtccactcca gtgtcgtcgc acccacgccc catggattct
    21961 cggctgcacg ctccttgcgc aggaaggcca ccaccatacc cacgaagaac atcgacgtgc
    22021 cgaacgccgt gatgtaggag cccatcgagg agatatagtt ccaataggcg aaggcatccg
    22081 ggtagtcggc gtaacgccgc ggcatgccgg ccaggccaag gaaatgctgc gggaagaaca
    22141 ggatgttcgc gccgatgaac gtcagccaga agtgcagctt cgcgaagaac tccgagtaca
    22201 tgtacccggt catcttcggg aaccaatagt accagccggc gaagatcgcg aagaccgcgc
    22261 cgagcgacag cacgtaatgg aagtgcgcga cgacgtagta ggtatcgtgg agcgcacgat
    22321 cgacgccggc attcgcaagc atcactccgg tgacgccgcc gatcgtgaac aggatgatga
    22381 agccgattgc ccacagcatc ggagcgcgga agtttatgga cccgccccac atcgtggcga
    22441 tccaggagaa caccttcacg cccgtgggca cggcgatcac catcgtgccg aacacgaagt
    22501 acgcctgggc gtcgacgccc atacccgaca cgtacatgtg gtgagcccat acgacgaacc
    22561 cgacgagccc gatcgcaacc atggcgtaag ccatgccgag atagccgaaa actggcttgc
    22621 gggagaacgt cgacacgacg tggctgatca tgccgaagcc cggcaggatc atgatgtaga
    22681 cctccgggtg gccgaagaac cagaagaggt gctgatagag gaccgggtca ccgccgccct
    22741 gggcatcgaa gaacgtggtg ccgaagttgc gatccgtcag cagcatggtg atggcgccgg
    22801 ccagcacagg gagcgacagc agcagcaaga aggccgttac cagaatcgcc cagacgaata
    22861 gcggcatctt gtgcatcgtc atgcccggcg cgcgcatatt gaagatcgtg gtgatgaagt
    22921 tgatcgcgcc gagaatcgag gaagcgcccg ctatgtggaa tgagaggatt ccaaagtcga
    22981 cggacggccc cgagtgaccc agagtggaaa gcggcgggta gagcgtccaa cccgttccgg
    23041 agcccatgtc gccggatggc ccctcgacga aaagcgagat gatgaaaagg ccaagggcga
    23101 ccggcaacaa ccagaacgag atattgttca ttctggggaa tgccatgtcc ggcgcgccga
    23161 tgtgcagggg cacgaaccag tttccgaacc cgccgatcat cgccggcatg atcacgaaga
    23221 acaccatgat gatgccgtga gcggtcacca tcgcgttgaa ggcgtgcgga ttggcgaaat
    23281 actggagccc cggttcctgc aactccaagc gcatgccgac cgataaagcg ccaccgatca
    23341 cgcccgcgat catggcgaag acgaggtaca tcgtaccgat gtctttgtga ttggtcgaaa
    23401 atagccagcg cttgatgccg tgcggcgtgt gatcgtcgcg aatgtgcgct gcggaagccg
    23461 tgctagccat ttgcagtcac ctttccctcg gtgtcaaaat tccgctattt cagtcgcggg
    23521 gcggcgtttg cccgcgcccc atgcaactca gttctgctcg acgcgatcag cagtgccggg
    23581 cggagccgat gccaccttgg ccgcgtcggg ctgatcggtc gcagcacgca aaatcccgcg
    23641 cgcccggtcc cagtctctgg ccttcgccgc ctcgatccac tggttgaatg cttcctcgct
    23701 gacgacgcga atggcgatcg gcatggaggc gtggtccttg ccgcaaagct ccgagcactg
    23761 gccgtagtag acaccgggcc gcgtcacctt gaaccaggtt gcagtgacgc gtcctggaac
    23821 cgcatcgacc ttcgaaccga aggacggaat ggtccagctg tggatgacgt cgtttgccgt
    23881 aacaagcacg tgcacggtct tgttcaccgg cactgcgatc tcgttgtcca ccgccagctg
    23941 acggggctgg ccgctcttcg cccagagggg cgccgcatcg ttgtagaggc gacgggtccg
    24001 ctcgacaccc gtcaaatcgc cgtagcgctt attgaactcg tcgtcaccga gctccgcccg
    24061 gagaagatcc tcgtcgcgga gaatgttgga ggtgaccgtg aggccatcgt agtcagggta
    24121 ctcgtgctcc cagaaccagg cattgccgat ggctttgatg gtcaggtctg gctttggata
    24181 gctgtactgc agatagagca gcttgaagga ggggactgcg atcacaacga gaatgaggat
    24241 cgggatcacg gtccagacca gctccagcat cgtgtggtgc gtggtccggg atggggtcgg
    24301 attggacttc tcgttgaagc ggaagatcac gtacagcagc aggatgccga cgaagacggc
    24361 gatcgcgatg atgagggtgt tcaccaggtc atggaacgcg tgaatgccct ccgccacctc
    24421 ggtcaccgcg ccttgcatgc ccagctgacc tggcgagggt tgaccgatgc ctgcccatgc
    24481 cgccgttgcc attcccagag ccgcgagcgc cgctattgaa cccagcgccc aaattcccaa
    24541 gctcgatttg cccattgccc tcgctcccac cgtcccgcga cttccgttgc ggaggcatat
    24601 caacttggat tgtcgatgat tgttgatcgt caacgttccg agtttgtcac agaaccataa
    24661 tggtcccaga tatctcaagc gccagttatg cggcatttcc acgcgcgacg cgcatgattc
    24721 acaggcccat cgccgcccga gttgcacgcc gacacgagac cctctgtcca agtcccactt
    24781 tagcccaatt tcctcagcag acaggctcga gtttggtcgt tccgccagac tagcccatcg
    24841 ggtcatcagg ctcatagaag aacggagagt gcagttggtc gaggccgtcg ccgggtgcgc
    24901 gtcgcgttct tctcgcgggc tctatcgcgc cggcgctgcc gcctttttcg ccctgctgct
    24961 gatgggctcg ttcgccgccc cagccgcacc agcaattgct caggcgccgg acggaaccgt
    25021 gaaggcccag cacggcgatt ggcaggtggt gtgcaaggcg ccactgccgg gtgccaagaa
    25081 cgagacgtgc gcacttgttc agagcgtcac cgccgaggat cggaacaacg tcggcctgac
    25141 ggtctatttc cagaagttct cgaacggcac gcgggtgctg cgcgtgtttg cgcccgtagg
    25201 tgtgctgctg ccacctggcc tcggcctgaa gatcgacggt aaggacgtgg ggcatgcgcc
    25261 tttcctgcgc tgccacgctt tcgcctgcta cgcgcaagtc gttgcggaag atggccttgt
    25321 cgagcagctc aaggccggca agacggccat cttcattata ttccagaccg aggaggccgg
    25381 gatcggcatc ccgatctcgc tggccggctt ctccgaagca ttggctgagc tccactagct
    25441 ctcgtcggcg ctcggcgcgc cagttcctca cttattcgga gtcacgctcc ctgatgagtg
    25501 cgtgcgatgc ccttgtgctc gggtgcccga gaaaatgcgt tccgagttgc tgatcctgcc
    25561 ttttaattgc cgcatgaagc cgctttaggg tcgcaaagcc gccggcaaaa aggacggttt
    25621 ctctcctgcc ttccgccatc ttgcgcggct agcaaggccg tgcaggagga ccccatgcaa
    25681 agcaagcgcc gaacgacgac ccggaagaac gtgcagcatg cctcagtcgt ggtagccgat
    25741 ctcgacggcc gccgttcgca gctcttgcaa tcgttccact tctcctgccg ccgcaaggtc
    25801 gccgagatgg ccagccgtgc cgcgtgtttc gaggagcttg ctgaaacgtt ccctggcctc
    25861 ctcttcgcgc tcgccacgaa ttacggaacg gtggagtccc gtcgcgctgc catcgaccac
    25921 ctgatcgcag gctctcgcct gcgcgaggca gcgaacgctc tcggcctgcc ctggtggatg
    25981 cgcaagctgc cgccgcaggc gttcagcaag cggctcgaaa gcattcctga cgacccagcc
    26041 tttgcggccc gggtcgtcaa tcttctgccc gccgtaccag ccggctcggc gaagtggctc
    26101 gaccgcgtgc tcctcgccta ccgggcctgc ggacccgagt ttgccctctg ggtggccaag
    26161 cacgaccggt tccacgcgcc gcttctgacc gatccggccc tgatctatgt cgcggcgtgg
    26221 gcttggtttg ccggccagcc ggacacgctg ggtgcaaggc tcctgcgccg gccctggacg
    26281 ccggccatga gcccgcgccg agcgttcgat gagctcacca cctggcgcaa gcgcgtccgg
    26341 ctcgctctga cgctcagcgc actcgaccgg cagccgtggc tcgcagaggg ctcggcgctc
    26401 ggctacgact tcgtcgagct gagggagatc acggatttca tcgccgagag cgacgcgatg
    26461 gacaactgcc tcgacagctt ctcggaaaaa ctcgagtcgg gaacgagcta cgtcttcagc
    26521 atccgccaca aaggcacgcc cgtcgccgac gtcgagatcg gcgcgcaccc gatcgagccg
    26581 aacctgccga ccatcgtgca gctgagggcg cctcgaaaca ggcgggcgag ccctcagatc
    26641 tggcgcgctg cctatgcctg gctcggctcc cagccgctgc ggcccgcgcc gccgctcgtc
    26701 atcaactcgg ccgcgcgtcg ccgcacctgg cgggagctgt ggagccccta tctttcggtg
    26761 ctcgcgcccg gtgaccggga acagttcgaa aaactggcca aggacctgga caagctgcgc
    26821 gcccgccgcg ggcggccggc gcgtgcgcgc cggacttcgc ccgctcagca gaccgtatcg
    26881 cctcagaccg aagaggccga cgtcgccatg gtctaagcgg cgccggctcc gtttttggca
    26941 gccggtggcc gccccattgt tgggtcttgg ctctattccg ccgctgcccg caggattggg
    27001 gccgccgcct tcacgtcggc gtcgacgtgg tcctcgaact tcctgaagtt cttgttgaac
    27061 atatcgacca gcgccctggc ctgggcgtcg taggcggctg ggtcagtcca ggtctcccgg
    27121 gggttgagga tcttctcctc tattcccggc agtgaggtcg gcaccgcgaa cccgaacgtc
    27181 gggtccgtcc gcatggcctg ccccttcagc ctgccggaaa gggccgcatc tagcagcgcg
    27241 cgggtcactt ggatcggcat cctgtggccg acaccatatt tgccgcccgt ccatccggtg
    27301 ttgaccagcc agcagtccac gccgtgcttg gcgatcagct cgcgaagcat gttgccgtag
    27361 acggtgggat gacggggcat gaacggagca ccgaagcagg tcgagaacgt ggcctgcggc
    27421 tcggagccga cgcctttttc cgtgcccgcc accttcgccg tgtagcccga gaggaagtga
    27481 tactcggcct ggctcggcgt cagctttgcg atgggcggca ggacgccgaa ggcgtcagcg
    27541 gccagcatga tgatgttctt cgggtgcccc gcgcggccgg tcgggctcga gttgcggata
    27601 aactccagcg ggtaggccgc tcgcgtgttc tccgtgagcg agccgtcgtg gaagtcgggc
    27661 tcgcgcgtgc cggccttgac gacgacattc tccag
//
LOCUS       DASVDU010000236        15272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_794484, whole genome shotgun sequence.
ACCESSION   DASVDU010000236 DASVDU010000000
VERSION     DASVDU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15272
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794484"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VF226_15115"
     CDS             complement(<1..945)
                     /locus_tag="VF226_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7075368.1"
                     /translation="MAADRVERGTTRIKGFQSGELDFQLMRSLGAGNYGGATPGEIFA
                     TRSAVAGDDPYAWRNALAGMGERVLNAGREALKRGHRVSARDHFLRASMYYRAAEYFA
                     DPFGSEALPWGLASRDAFRAAAELMRDRIEPVEIPFEGKALPGYFMAPAGGAERGKTI
                     LILTGFDGTGEELYFQTAAAALDRGYNVLVGEGPGQVGCLRQHPDLKFRPDYEKPIGA
                     MLDFVLARPEVGSERLALYGISFGGYFVTRAAEHDGRIKALIANSPIIDLYAYMAGFV
                     GPEFVANPPPVSLSEVDEIPDEEFPREAKLSFKSACRRF"
     gene            1349..1663
                     /gene="rplU"
                     /locus_tag="VF226_15120"
     CDS             1349..1663
                     /gene="rplU"
                     /locus_tag="VF226_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7075369.1"
                     /translation="MFAVIKTGGKQYRVATDDVLTVEKLDGEAGSEVEFTEVLMIGGN
                     GTPKIGTPTVPGAKVTAELVEQTRGPKLIAFKKRRRKNSRRKKGHRQDLTKVRITNII
                     GA"
     gene            1696..1968
                     /gene="rpmA"
                     /locus_tag="VF226_15125"
     CDS             1696..1968
                     /gene="rpmA"
                     /locus_tag="VF226_15125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7075370.1"
                     /translation="MAHKKAGGSSRNGRDSHSKRLGVKKYGGEFVVPGNIIVRQRGTQ
                     WHPGDNVGMGTDHTLFAVAEGHVEFRTKAKGRVFVSVRPPKLEAAE"
     gene            2168..2683
                     /locus_tag="VF226_15130"
     CDS             2168..2683
                     /locus_tag="VF226_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7075371.1"
                     /translation="MRTKRLLLRDLTLRDVRRIAMLASDWDVARMTGRIPHPYSEELA
                     RQWISDLTEGEFVQGIVSQGELVGLCGYMPDGNSAEIGYWVGKPWWGRGFATEAAGAL
                     IDYCFNEVGFERITCGHFVDNPASARVIAKLGFRFTGWERVWCEARQSDVDTLKYERL
                     RQAGEAKVRVS"
     gene            2680..3723
                     /gene="obgE"
                     /locus_tag="VF226_15135"
     CDS             2680..3723
                     /gene="obgE"
                     /locus_tag="VF226_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX7075372.1"
                     /translation="MKFLDQAKIYVKAGDGGPGAVSFRREKFIEFGGPDGGDGGRGGD
                     VWIECDPNLNTLIDYRYQQHFKAKNGQHGMGKNRSGAKGADCVLKVPPGTQVFAEDQE
                     TLLADLTKPGQRVRIARGGNGGFGNAHFKTATNQAPRRANPGQPGEELTIWLRLKLIA
                     DAGLIGLPNAGKSTFLSKVSAAKPKIADYPFTTLHPNLGMVRVGDIDFVLADIPGLIE
                     GAHAGAGLGTRFLGHIERCRVLLHLVDATEEDVADAYRTVRRELKAYAADLEKKKEIV
                     ALSKCDALDPQTLEEKQEVLKRAARKKPHLLSSVSGQGVEEILYALARAIETSREDER
                     LAAEPVEAREWRP"
     gene            3720..4898
                     /gene="proB"
                     /locus_tag="VF226_15140"
     CDS             3720..4898
                     /gene="proB"
                     /locus_tag="VF226_15140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX7075373.1"
                     /translation="MNQNSAAAVGEVGESAPDAGRAAWAEARRIVVKIGSALLVDKGS
                     GRIRSTWLNSLADDVAELRQEGREVVLVSSGAIALGRHILKLPKGPLQLEQSQAAAAV
                     GQITLARSYQELFRARGLITAQVLLTLGDTEKRRRYLNARNTIDTLLAHGAIPVVNEN
                     DTVATSEIRYGDNDRLSARVASMISADALVLLSDVDGLYTSPPGTDPQARRLDVVRAI
                     TPEIEAMAGDAGTELSRGGMKTKIEAGRIALSAGTDMVIASGKVLHPLRALRDGVPCT
                     WFLAPSDPVTARKRWIAGQLEPRGTVVIDPGAERALLAGKSLLPAGVTRVEGQFDRGD
                     AVIIRNNSGREIGRGLIAYAHDDAARIIGRRSSEITEILGHEGRSELIHRDNMVLNRK
                     "
     gene            4904..6196
                     /locus_tag="VF226_15145"
     CDS             4904..6196
                     /locus_tag="VF226_15145"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX7075374.1"
                     /translation="MNRPDRNIQGAETADLMRGMGLAARAASRQIALAPPAQKNEALT
                     AAAKAVWDGASAILEANAKDLEAARSRGTTAAFLDRLTLNKSRIEAIARSLEDIAALD
                     DPVGTVLARWKRPNGLVIERVRVPLGVIGIIYESRPNVTADAGALCLKSGNAAILRGG
                     SESRHSSRAIHACLAQGLKAAGLPEAAIQLVATTDREAVGEMLKGLDGNIDVIVPRGG
                     KSLVARVQDEARVPVFAHLEGICHTYVDKDADLDMAREIVLNAKMRRPGICGATETLL
                     VDRAAPANVLPALVKALIEAGCAVRGDEAAQAADPRVTPATEEDWSTEYLDAIISVKV
                     VDGVDGAIDHIARYGSQHTDAIITNDAASAERFLQRVDSAIVLHNASTQFADGGEFGF
                     GAEIGIATGKMHARGPVGVEQLTSFKYVVRGAGQVRPA"
     gene            6403..7092
                     /locus_tag="VF226_15150"
     CDS             6403..7092
                     /locus_tag="VF226_15150"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7075375.1"
                     /translation="MRRSAPVRFGSVRVRTPLVTSGQRVGVMGGTFNPPHAGHVLISH
                     MALTRLRLNQLWWVVTPGNPLKETGDLPPLEERMAACRAMTADPRIKITGFEEQLGTP
                     YTAATLAFLARRYPSVRFVWVMGADNLASFHRWQHWREIMHLMPIAVIDRPGWRHRAL
                     ASKTAHAFRDAYVPETRSGDLPDLTPPAWTLLTGPLSPLSSTAIRAMRRANATDRQQY
                     VAKAQPFGGQG"
     gene            7300..7647
                     /gene="rsfS"
                     /locus_tag="VF226_15155"
     CDS             7300..7647
                     /gene="rsfS"
                     /locus_tag="VF226_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7075376.1"
                     /translation="MGSEALLTHVAHWLDEAKAEDVVTIDLRGKSSIGDFMVIATGRS
                     DRHVGAIADQIQRKLKEHGFGRVRVEGAEACDWVLIDAIDVIVHVFRPEVREFYNLEK
                     MWLSDRPADGAQH"
     gene            7705..8187
                     /gene="rlmH"
                     /locus_tag="VF226_15160"
     CDS             7705..8187
                     /gene="rlmH"
                     /locus_tag="VF226_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX7075377.1"
                     /translation="MRIIIAAVGRLKDGPERQLVQRYRDRLAASGRALGWGPVDIIEI
                     PESRAPDPSTRKSEEAARLLAKVGEAGKMVALDENGREFTSDEFAVYLRMQRESGVRS
                     LAFLIGGPDGHGAEVLADADLRLSFGRMTLPHMLVRVLLVEQLYRAATILSGHPYHRS
                     "
     gene            complement(8208..9353)
                     /locus_tag="VF226_15165"
     CDS             complement(8208..9353)
                     /locus_tag="VF226_15165"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7075378.1"
                     /translation="MDFDLSEEQRMALDAWRRFIDRDIRPITAEYRDTLIPAPVLKKL
                     LGMTAEYGVGCGWVPEDGGGAGLDFLTSGLLYEELARVSPDLAGAAFVNEGAAIKVYN
                     GGSEALKARYLGPTLRGELIGCSAISEPDVGSNVRAMRTRAFRDGDHYRINGEKMWIS
                     NCSVADYATVVAATGDQEFTMFLVDRAEHGFETREIEKIGLNGWSLGQIILNDVSVPA
                     ANMIGGLGGGLRETMKGFERSRCFISLLGLGMAQASLEASISYAQQREQFGKPIGGHQ
                     LVQQLIAEMATELEAARLLVYRALSQLTLGKGTNIEAAMAKAFATEAAVRITSKAIQV
                     HGASGISREFPVERHFRSARMLTIPDGTTQINNLVIGRTLLGINAFG"
     gene            complement(9357..10133)
                     /locus_tag="VF226_15170"
     CDS             complement(9357..10133)
                     /locus_tag="VF226_15170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7075379.1"
                     /translation="MSILLYEERQGIALLTLNRPESLNAISSALAEAMAEKLEEIAGR
                     RDIRAVVITGAGEKAFSAGTDLKERRTLDPDGKWQQRTRGWRVNRLLHELPQPAIAAI
                     DGWCLGGGFELALFCDLRVATERAVFSFPEMTLGAYPGSGAAIALPRLIGRARAKELF
                     FTARRVGAAEALDLGIVEWVVPPGRHLAKALEIAEQVKVTSPLGVAAVKRMVNEGADL
                     DFDGAVALNERLRRPLESTRDYEEGIQAFFEKRQPVFRGE"
     gene            complement(10149..10565)
                     /locus_tag="VF226_15175"
     CDS             complement(10149..10565)
                     /locus_tag="VF226_15175"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7075380.1"
                     /translation="MSKSGIDAVPIGASAQITKTVSESDVYLYAGISGDFSPNHVNEQ
                     YMREGRYGRRIAHGTLLMGFMSAASARLQLGRTVSLGYDRVRFPAPVFLGDTITTVYT
                     IREVNREKRRVIADVRCTNQRGEVVGAAVNIRAFVD"
     gene            complement(10591..11751)
                     /locus_tag="VF226_15180"
     CDS             complement(10591..11751)
                     /locus_tag="VF226_15180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7075381.1"
                     /translation="MQKALSGIRVLDFTRLYAGPFCTMLLGDLGADVVKVEAPGGDPI
                     RHQGPPFYHGHGMSFLAVNRNKRSIVLDMKTPSGRETAQRLAATADVVVENFRPGVMD
                     RLGLGYEALAALNPRLIYATMSGMGADGPDRDKGAFDLTIQAEGGYMSITGERGGAPI
                     KLGTSAFDLICGQYAMGAIVTALFARERTGRGQRIETSLLESEVSFLVDAAMEWFLAG
                     SIREKWGSEHPGQVPYKAFRTADGWLIIAAGMQNLFESFLSVLRREDLLEDERFATLP
                     ARVANRDVVHEILDAEVRRYTTADLQAALERVQVPCAPVNDIRQVFEHPQVLHRGMRR
                     EVEHRAYGRIPVIGPAVKYGNFDVAAEWRAPPVLGEHTEEVLQEWLSEQRAS"
     gene            complement(11775..13400)
                     /locus_tag="VF226_15185"
     CDS             complement(11775..13400)
                     /locus_tag="VF226_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7075382.1"
                     /translation="MTTDVLVLGGGIAGYRAAVAAREADCSVVLASLAHGASPYVIGA
                     NIPLGASDPADGPQVYFDDMVRGGYGLNDRRLVQAMAAQAVPSFRELDGLGVPFARDN
                     GRVLQRHLSGNTYPRSVFVPEGTGRVILDYLKARAKAIGVATCQGWKVVSLLSDQGEV
                     VGALLVKRHTGELLAIRARATILAMGGIGRIYDDSTYPADIAADSYALAYDVGARLVD
                     MEFVQFEPVVTVWPEECRGLEMPTAMLGDGARLINAKGERFMFRYNPEHGEKRIEKAR
                     MSLCIQQEIDEGRGFPDGTVLFDTTSVPRDRLESYVSHCKRLRAAGVDPAVEGPRVRP
                     AAHSQMGGVHIDHHGWSGVPGLYACGEAAGGVHGASRLAGNGGSETIIFGAIAGRGAA
                     SGLLAPAARDWSRIERMAVDSLRAAGRTHGSAEPIEIKRAVRQLMARAAGLYRDATGL
                     AGGIADADRLQADVDAGLKVESTSESVAALEARNMLLIARLILRAALARTESRGAHQR
                     RDHRQQDDSAWLKHLSYRRGPDGQPVQELVAIQ"
     gene            complement(13440..>15272)
                     /locus_tag="VF226_15190"
     CDS             complement(13440..>15272)
                     /locus_tag="VF226_15190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX7075383.1"
                     /translation="HLWTSYAGTLPTMQQAYAHLGFALVLIFLTRPFVAEGSKARTLL
                     RWSIDSLLILGSVAISAYAVINFLEISRRGAGDPTEVGVWLGVLAVILVIDATRRVVG
                     WALPILAILFLIYGFVGPYMPGALGHRGYDLERIASTLYLTTSGLAGTPLQVSATYVA
                     IFIIFAGFLDTSGAGKFFIDWSYAGLGWMRGGPAKVAVVGSAMMGTISGSAVANVVAT
                     GTFTIPVMKRAGLRPAFAGAVEAAASSGGQIMPPVMGAAAFIMVEFLGAPYSEIMTAA
                     IFPGILYFLAVLTMVDFEVAKAGIKGLPRSELPSAWGIFKGGWHLLAPLVLLVYLLVV
                     IEYTPVTSAFYGIVAVLAVSFLRGATRMYPDRIWTALRASGTGMLEVAAACACAGIII
                     GILMLTGLATRLSSIMIDLAGGSLFVLMIITMIISLILGMGLPTSAVYIILATLVVPA
                     MVEMGVNPLAAHMFVFYFGVLANVTPPVAIAAYAGAGLAGANATTTGVIAFKLALAGF
                     LLPYIWVYNPALLLQGTPFVIALAVVTSLVGIIALAAAVQGYLLGGPANWLQRLLLGG
                     GALALIKPGILTDLIGFGLIAVALLLWWIWRPAPQTPQAASASE"
BASE COUNT         2844 a         4886 c         4986 g         2556 t
ORIGIN      
        1 gaacctgcgg caggccgact tgaagctgag cttggcttcg cgggggaact cctcgtcagg
       61 gatctcgtcc acctcgctca gcgacacggg cggcggattt gccacaaact cgggtccaac
      121 gaaacctgcc atgtaggcgt agaggtcgat gatcggcgag ttggcgatga gcgccttgat
      181 gcgcccatca tgctcggccg cgcgcgtgac gaagtagccg ccgaagctga tgccgtagag
      241 ggcgagacgc tccgaaccca cttccggtct ggcgagcaca aaatcgagca tcgccccgat
      301 cggtttctcg tagtcggggc ggaacttcag gtccggatgc tggcgcaggc agccgacctg
      361 tcctgggcct tcgccgacaa ggacgttgta tccgcgatcg agcgccgccg cagcggtttg
      421 gaaatagagt tcctcaccgg tgccgtcgaa gcccgtgagg atgaggatgg tcttgccgcg
      481 ctccgccccg ccggcgggcg ccatgaagta gccgggcaga gccttgccct cgaaaggtat
      541 ctcgaccggc tcgattcggt cccgcatcag ctcggctgcg gcacggaacg catcgcggct
      601 ggcgagcccc caaggcagcg cttccgatcc gaaggggtcg gcgaagtatt cggcggcgcg
      661 gtagtacatg gaagcgcgca ggaaatgatc ccgggcgctg acgcggtggc cgcgcttgag
      721 cgcctcgcgg ccggcgttca ggacccgttc gcccattcca gccaaggcgt tccgccaggc
      781 gtagggatcg tctccagcaa ctgccgagcg ggtcgcgaaa atttccccag gggtcgcacc
      841 accatagttg ccggcgccca gggaccgcat gagctggaaa tcgagctcgc ccgattggaa
      901 gcccttaatg cgggtcgtgc ctcgttcgac gcgatctgct gccattccga cctccctggt
      961 attcccggac ccggaatgta ggacgctggc tcccagagcc gccatcgtcg cgagaatgca
     1021 ggtccgtcgc gttatgatcg cccctgtcat gatcgcctgc tcctacccag gcgaatcaac
     1081 gcgcgcaggc cttaaaatcg ggtgaatgcg tccggagccc gctgggacgg gcgagcctat
     1141 cctaatgttc cggcattgac ttgcaggcgc ttcccgacga tattgccgcc gcacgcggtg
     1201 gcttggggcc atcgcgcaag aggcgctgcc cgaggctctt cgccggcatc cggccggtag
     1261 accgtttccc gacaggtgag gcggttcatc caagtccgcg acgtgacacg cgcggcgccg
     1321 cagggcgtgg ataagacaag gatacgtcat gttcgcagtc atcaagaccg gcggcaagca
     1381 gtaccgcgtg gcgaccgacg acgtccttac cgtcgaaaag ctcgacggcg aggccggctc
     1441 cgaggtggag ttcacggagg tgctgatgat cggcggcaac gggacgccga agatcggcac
     1501 gccaactgtc ccgggagcca aggtcactgc ggagctcgtg gagcagaccc gcgggcccaa
     1561 gctcatcgca ttcaagaagc gccgccgcaa gaactcgcgg cgcaagaagg ggcatcgtca
     1621 ggatttgacg aaggtccgta tcaccaacat catcggcgcc tgaagggcag ccgacccgac
     1681 cacgaggttt gagacatggc acacaaaaag gcaggcgggt cgtcgcgtaa cggccgcgat
     1741 tcgcattcga agcgcctcgg cgtgaagaag tacggcggcg agttcgtcgt gccgggcaac
     1801 atcatcgtgc gccagcgcgg cacgcagtgg catcccggcg acaacgtcgg catgggcacc
     1861 gaccacacgc tgttcgcggt ggccgagggg cacgtggaat tccgcaccaa ggcgaagggg
     1921 cgtgtcttcg tgtccgtgcg accgcccaag ctcgaagcgg cagagtgaag ccgcacacag
     1981 gcggtggctt gttgaaggga gatggcaggc catctccctt ttttgatggc catcgccaag
     2041 ccggatcaga ggcgccgaag gcgcgaagcg tgttcttagg acgagcgtca cgctggaccc
     2101 ccggtttgac gtcgttatgc tcgccgactt gaaaacggat gtgatctgtg cccgctgagc
     2161 tccgcacatg agaacgaagc gattgctcct gcgagacctc acgctccgcg acgttcgccg
     2221 gatcgcgatg ctggcgagcg attgggatgt cgcgcgcatg accggccgca tcccgcatcc
     2281 ctactcggag gagctggccc gtcagtggat cagcgatctt accgaagggg aattcgtgca
     2341 aggaatcgtc agccagggtg agctcgtagg gctctgtggc tacatgccgg acggcaattc
     2401 ggccgagatc ggctactggg tcggcaaacc gtggtggggc cgcggattcg ccaccgaggc
     2461 ggccggagcc ctgatagact actgcttcaa cgaagttggc ttcgaacgga tcacctgcgg
     2521 ccatttcgtc gataatcctg catccgcccg ggtcatcgcg aagctcggct tccgttttac
     2581 gggttgggag cgcgtctggt gcgaagcccg ccagtcggac gtggatacgt tgaaatacga
     2641 gcggttgcgc caggccggcg aagccaaggt cagggtgtca tgaagttcct cgaccaggca
     2701 aaaatctacg tgaaggctgg tgacggtggc cccggcgcgg tgagcttccg ccgcgaaaaa
     2761 ttcatcgaat tcggcgggcc cgacggcggc gacggcggca ggggcggaga cgtctggatc
     2821 gagtgcgacc ccaatctcaa tacgctgatc gactaccgct accagcagca cttcaaggca
     2881 aagaacggcc agcacggcat gggcaagaac cgctcaggcg ccaagggggc cgactgcgtt
     2941 ctcaaagtgc cgccgggaac gcaggttttc gcggaggatc aggagactct gctggcagac
     3001 ctgacaaagc ccggccagcg cgtgcgcatc gcccgcggtg gcaatggcgg tttcggcaat
     3061 gcccatttca agacggcgac gaatcaggcc ccgcgccggg ccaatcccgg ccagcccggc
     3121 gaggagctga ccatctggct gcgtttgaag ctgatcgccg atgcaggcct catcggactg
     3181 cccaatgcgg gaaagtcgac gtttttgtcg aaagtcagcg cagccaagcc caagattgcc
     3241 gactacccgt tcacgacgct gcaccccaac ctcggcatgg tccgcgtggg cgacatcgat
     3301 ttcgtgctgg ctgatattcc agggttgatc gagggcgcgc acgccggcgc cgggctcggt
     3361 acgcgctttc tggggcacat cgagcgctgc cgcgtgctgc tgcacctggt tgacgccacc
     3421 gaggaggacg ttgctgacgc ctatcgcacc gtgcgtcgtg agctgaaggc ctatgctgcg
     3481 gatctcgaaa agaagaagga gatcgtcgcg ctatcgaaat gcgatgctct cgatccgcaa
     3541 accctcgaag agaagcagga ggtcctgaag cgggccgcgc gcaagaagcc acatcttctt
     3601 tcctccgtgt ccggacaagg cgtagaggaa atcctctacg ctctggcgcg tgccatcgag
     3661 acctcacgcg aagatgaacg gctggcggcc gagcccgtcg aggcaaggga atggcggcca
     3721 tgaaccagaa tagcgctgct gctgtcggcg aagtcgggga atccgccccc gacgccggtc
     3781 gcgccgcatg ggctgaggcc cggcggatcg tcgtcaagat cggctctgca ttgctggtcg
     3841 acaaaggctc cggccgtatt cgctcgacct ggctcaattc cctggctgac gacgtggcgg
     3901 agctgcggca ggagggacga gaggttgtgc tggtctcctc gggggccatc gcgctcggcc
     3961 gacacatatt gaagctgccc aaaggccctc ttcagctcga gcagtcgcag gcggcggccg
     4021 ccgtcgggca gatcactctg gcgcgttcct atcaggagct gttccgggcg cgtggcctca
     4081 taaccgccca ggtgctgctg accctcggcg acaccgagaa gcgccgccgc tatctcaacg
     4141 cccgcaacac gatcgacacg ctgcttgccc acggcgccat cccggtcgtc aacgagaacg
     4201 acacggtcgc gacgagcgag atccgctacg gcgacaacga ccggctgtcg gcacgggtgg
     4261 ccagcatgat cagcgccgat gccctggttc tcctttccga tgtcgatggc ctctatacca
     4321 gcccgcccgg cacagacccg caggcgcgac ggctcgacgt ggtccgcgcg atcacgcccg
     4381 agatcgaggc catggctgga gatgccggca ccgagctgtc gcgcggcggc atgaagacca
     4441 agatcgaggc agggcgcatc gcgctttccg ccggtacgga catggtgatc gcctcgggca
     4501 aggtgctgca tcctctgcgg gcgctgcgtg acggagtgcc gtgcacatgg ttccttgcgc
     4561 cgtcggatcc ggtgactgcc aggaagcgct ggattgccgg acagctcgag ccccggggaa
     4621 cggttgtgat cgacccaggg gcggagcgcg cgctgctcgc gggcaagagc ctgctgccgg
     4681 ccggcgtgac gcgcgtcgaa ggccagttcg accgcggcga tgccgtgatc atccgcaaca
     4741 atagcggtcg cgagatcggc cggggcctga tcgcctatgc gcacgacgat gcagcgcgta
     4801 tcatcggccg ccgaagcagc gagatcactg agatcctcgg ccatgagggc cgtagcgagc
     4861 tcatccatcg cgacaacatg gttctgaatc ggaagtagcc gccatgaaca ggccggatcg
     4921 caatatccaa ggcgcggaaa cggcagatct gatgcgcggg atggggctcg cagcgcgagc
     4981 agcctcgcgc cagatcgcgc tggctcctcc ggcgcagaag aacgaggcgc tcacggctgc
     5041 cgcaaaagct gtgtgggacg gagcttcggc cattctggag gccaacgcca aggacctgga
     5101 ggccgcacgc agcaggggca cgaccgcagc ctttctggac cggctgacgc tgaacaaatc
     5161 ccggatcgaa gcgatcgcac ggagcctcga ggacatcgcc gctctcgatg atccggtcgg
     5221 caccgtcctg gctcgatgga agcggccgaa cggcctcgtc atcgagcggg tacgtgtgcc
     5281 gctgggcgtc atcggcatca tctacgagag ccggcccaac gtgactgcgg atgccggtgc
     5341 gctttgcctg aaaagcggga acgccgccat tttgcgcggc ggctctgaaa gccggcattc
     5401 gagccgggcc attcacgcct gcctggcgca aggcctcaag gctgctggcc tgccggaagc
     5461 cgccattcag ctcgtggcga cgacggatcg cgaggcggtt ggcgagatgc ttaaggggct
     5521 cgatggcaat atcgacgtca tcgtaccccg tggcgggaag agcctcgtcg cccgggtgca
     5581 ggacgaagcg cgcgtgccgg tcttcgcgca cctcgagggg atctgccaca cttacgtcga
     5641 caaggatgcc gatctcgaca tggcgcgcga gatcgtgctg aacgccaaga tgcgccgtcc
     5701 gggcatctgt ggggcaaccg aaacgctgct ggtggaccgc gctgcgcccg cgaacgtcct
     5761 gccggcgctg gtgaaggcat tgatcgaggc cggctgcgcc gtgcgcggtg acgaggcggc
     5821 gcaagccgct gacccgcgcg tcacgccggc cacggaggag gactggtcga ctgaatatct
     5881 ggatgccatc atttcagtga aggtggtgga cggagtcgat ggcgcgatcg atcacatcgc
     5941 ccgctacggc tcacagcata ccgatgccat catcaccaac gacgcagcca gtgcggagcg
     6001 tttcctgcag cgggtcgatt cggccatcgt cctgcacaac gcttccacgc agttcgcgga
     6061 tgggggtgag ttcggcttcg gcgcggagat tggcattgcc accggcaaaa tgcacgcccg
     6121 cggacccgtc ggcgtcgagc agctcaccag cttcaagtac gtcgtgcgcg gtgccggcca
     6181 ggtgaggccg gcatgaccct ggcttttcaa agcacgatgg ggcctggccc gatactctgt
     6241 tcacctctcc catggggaga gatccgcccc cagggccgga tgaatcggga gaccctcctc
     6301 acccctaccc tttatccgtc ggggagaggg agcccggctg tgcgcttggc gcctgtcgat
     6361 ccaatccaga aaagcccctc tcggattgcg agggcctgag ggatgcgcag gagcgcgccg
     6421 gtccggttcg gctctgtcag ggtccgaaca ccgctcgtca catccggtca acgggtcggt
     6481 gtcatggggg gaacgttcaa cccaccccat gccggccacg tcctcatcag ccacatggcg
     6541 ctgacgcgcc tccgcctgaa ccagctctgg tgggtggtga cacctggcaa tccgctgaag
     6601 gagacgggcg acctgccgcc attggaggag cgcatggccg cctgtcgcgc catgactgcg
     6661 gatccgcgaa tcaaaattac gggcttcgag gagcagctcg ggacccccta tacggcggcc
     6721 acgctggcct ttctggcaag gcgctatcca tccgtccggt tcgtctgggt aatgggcgcc
     6781 gataatctgg caagcttcca ccgctggcag cactggcgcg agatcatgca tctgatgccg
     6841 atcgccgtga tcgaccggcc gggctggcgc catcgggcgc tggcctccaa gaccgctcac
     6901 gctttccggg acgcttacgt gccggagacc cggtccggcg atctgcccga cttgacgccg
     6961 ccggcttgga ccctgttgac cgggcctttg tctcccctgt catcgacagc catccgggcc
     7021 atgcgccggg cgaacgccac cgatcggcaa cagtatgtgg ccaaggcgca accgtttggc
     7081 ggccagggtt gaaaatccgt atcgaaacca cttagtctag agacggccgc cttggagggg
     7141 ccgccaacgg ctgttgccga gaccacatga gagtggagcg tggtcctcgg gtagcacccc
     7201 aacgggactt gatggcgaaa ggaaacccac gacacgatgc aagccgcttt tgagggcgcc
     7261 cgccgcggcg cgcctccagc cgaactcgtt tccgacggaa tgggttcgga agccctcctc
     7321 acccacgtcg cccactggct cgatgaggcc aaggccgagg acgtggtcac gatcgatctc
     7381 cgagggaaat cgtcgatcgg cgacttcatg gtgattgcga ccggccggtc ggatcgacac
     7441 gtcggggcca tagccgatca gatccagcgc aaactgaagg agcatggctt cggccgtgtc
     7501 cgcgtcgagg gcgcggaagc ctgcgactgg gtgctgatcg acgcgatcga cgtgatcgtc
     7561 cacgtcttcc gtccagaggt gcgcgagttc tacaatctgg agaagatgtg gttgtcagac
     7621 cgcccggcgg acggcgccca gcactgacgg ctcgaacgcg tctgacctga ttgcgcgggc
     7681 cggtcccgcg aaaggggggc ggccatgcgc atcatcatcg ccgccgtcgg acgtctcaag
     7741 gacggccccg agcgccagct cgtccagcgc tatcgcgacc ggctggcggc gagcggtcgt
     7801 gcgctcggct ggggacctgt cgacatcatc gagattcctg aaagccgcgc gccggacccg
     7861 agcacacgca agtccgagga ggctgctcga ctgctcgcga aggtcggcga agcgggaaag
     7921 atggtcgccc tcgatgaaaa cggccgcgaa ttcaccagcg acgaattcgc cgtgtatctg
     7981 cgcatgcagc gcgaatcggg tgttcgctcg ctcgcctttt tgatcggcgg accggacggg
     8041 cacggggccg aggtcctggc cgacgccgat ctcaggctgt cgttcggccg gatgacgctg
     8101 ccgcacatgc tcgtccgcgt tctactcgtc gagcagcttt accgcgctgc cacgattctg
     8161 tcgggacacc cttatcatcg aagctgatct ggcagtcggc aggaagatta gccaaaggcg
     8221 ttgatgccga gcagtgttcg gccaatcacc agattattga tctgagtcgt cccgtcaggg
     8281 atcgtcagca tgcgcgcgct gcggaagtgg cgctcgactg ggaactcgcg gctgatccca
     8341 gaggcgccat gcacctggat ggctttcgac gtaatccgca cggccgcctc cgtggcgaag
     8401 gccttggcca tcgctgcctc gatgttcgtg cccttgccga gtgtgagctg tgacagggcg
     8461 cgatagacca ggaggcgcgc cgcctcgagc tcggtcgcca tctcagcaat gagctgctgc
     8521 acgagctgat ggccgccgat cggcttgccg aactgttcgc gctgctgagc ataggaaatg
     8581 ctggcctcga ggctggcctg ggccatcccg agcccaagga gtgaaataaa gcagcgcgat
     8641 cgctcgaaac ccttcatcgt ctcccgcagg ccaccgccaa ggccgccgat catgttggcg
     8701 gcgggaaccg aaacgtcatt gaggatgatc tgcccgagcg accagccgtt caatccgatc
     8761 ttctcgatct cccgggtctc aaaaccgtgc tcggcgcggt caaccaggaa catcgtgaac
     8821 tcctgatcgc cggttgctgc cacgacagtg gcatagtcgg caaccgagca gttcgagatc
     8881 cacatcttct cgccgttgat gcggtagtga tcgccgtccc tgaaggcgcg cgtgcgcatg
     8941 gcacgaacgt tcgaaccgac atctggctcg ctgatcgccg agcagccgat cagctcgccc
     9001 cggagcgtcg ggccaagata ccgcgccttc agcgcctccg agccgccgtt gtagaccttg
     9061 atcgccgcgc cctcgttgac gaacgcggcg cccgcaaggt cgggagagac gcgtgccagc
     9121 tcctcataaa gaagaccgct cgtcaggaaa tcgagccccg ctccgccccc atcctctggc
     9181 acccagccgc atccgactcc gtactctgca gtcataccaa gcagcttttt cagcaccggg
     9241 gccgggatca gcgtgtcgcg gtattcagcg gtgatcgggc gaatgtcgcg atcgatgaag
     9301 cggcgccagg catcgagcgc catccgctgc tcttcggaaa gatcgaagtc catggcctat
     9361 tccccgcgga agactggttg gcgcttttcg aagaaggcct ggattccctc ctcatagtcg
     9421 cgagtgcttt cgagtggacg gcgcaagcgc tcgttaagag caactgcacc gtcgaagtcg
     9481 aggtcggctc cctcgttcac catgcgcttg acggcggcga cgcccagcgg gctcgtaacc
     9541 ttcacctgct cggcgatctc aagtgccttc gccaggtgcc gacccggcgg cacgacccac
     9601 tcgacaatgc cgaggtccag cgcttccgcg gcaccgaccc ggcgcgcggt aaagaacagc
     9661 tccttggccc gagcgcgtcc gatcagtcgc ggcagcgcta tggcagcccc cgatccggga
     9721 taagccccaa gcgtcatttc cggaaaagag aagaccgctc tttcggtggc aacacgaagg
     9781 tcgcaaaaca gagcaagctc aaagccgccg ccgaggcacc acccgtctat ggccgcaatg
     9841 gcgggttggg gcagctcgtg gagcagccgg ttgacacgcc aaccgcgcgt tcgctgctgc
     9901 cacttgccgt ctggatcgag cgtccgccgc tctttcagat ccgtaccggc cgagaaggct
     9961 ttctcacctg cgcctgtaat gacgacggcg cggatgtccc ggcggccagc gatctcctcg
    10021 agtttttccg ccatcgcctc ggccagggcg ctggagatgg cgttgaggct ttccggccgg
    10081 ttcagcgtca ggagcgcgat gccctggcgt tcctcgtaaa gaaggatcga cattcttccc
    10141 tcccagcgtc agtcgacgaa ggcgcggatg ttgacggcgg cgccgacaac ctcgccgcgc
    10201 tggttcgtgc aacggacgtc agcgatgacg cggcgcttct cccggttcac ctcgcggatg
    10261 gtatagaccg tcgtaattgt gtctccgagg aagacgggcg cgggaaagcg cacgcgatcg
    10321 tagccgagcg agaccgtccg cccgagctgg agccgcgccg acgccgcaga catgaagccc
    10381 atcagcaaag ttccatgggc gatgcgccgg ccgtagcggc cctcgcgcat gtactgctcg
    10441 ttgacgtggt tcggtgagaa gtcaccggag attcctgcat acaggtagac gtcggactcc
    10501 gacaccgtct tggtgatctg ggcggaggct ccgattggga cagcgtcgat acctgacttc
    10561 gacattcgct tctccttact cgcgcggccg tcagcttgcc cgctgttccg acagccactc
    10621 ctggaggacc tcctccgtgt gctccccgag gacgggaggt gcccgccact ccgctgcgac
    10681 atcgaagttt ccgtatttga ccgccggccc gatgaccggg atgcggccgt aagcccggtg
    10741 ctcgacctcc cggcgcatgc ctcggtgcag cacctgcggg tgctcgaaga cctgccgaat
    10801 gtcgttcacg ggtgcacaag gcacttggac ccgctccaag gcggcctgca gatcggcagt
    10861 cgtgtagcgg cgcacttcag cgtcgaggat ctcgtggacc acatcccggt tggccactcg
    10921 tgcgggcagc gtcgcgaaac gctcgtcctc caggagatct tcccgtcgga gcaccgaaag
    10981 gaaggactcg aacaaattct gcatgccggc ggcgatgatc agccagccgt cggcggtccg
    11041 gaaagccttg tagggcacct ggcccgggtg ctctgaaccc cacttctccc tgatgcttcc
    11101 ggcaaggaac cattccatcg cagcgtccac caggaagctg acctcgctct cgagcaggct
    11161 ggtctcgatg cgctgacctc tgcctgtgcg ctcgcgcgcg aagagcgccg tcacgatggc
    11221 gcccatggcg tactggccgc agatgagatc gaaggcggaa gtgccaagct tgatgggcgc
    11281 accgccacgc tcgcccgtga tgctcatgta tccaccttcg gcctggatcg tcaggtcgaa
    11341 agcccctttg tcgcgatcgg ggccatccgc acccattccc gacatcgtcg cgtagatcag
    11401 tcgcgggtta agggcagcga gcgcttcgta gccgaggcct aggcggtcca tcacgccagg
    11461 gcgaaaattt tcaacgacga catccgccgt cgctgcgaga cgctgtgcgg tttctcgtcc
    11521 cgacggcgtt ttcatatcga gaacgataga gcgcttattt cggttgacgg cgagaaagct
    11581 catgccgtgt ccgtgataga agggcggccc ctgatggcgg atcggatcgc cgccgggcgc
    11641 ttcgaccttc accacgtctg ccccgagatc gccaagcagc atcgtgcaga acggccccgc
    11701 gtagagccta gtgaagtcca ggacgcgaat accgctgaga gctttctgca tgggagaacc
    11761 gtgagttttg gtcatcattg gattgcaacg agctcctgga ccggctgccc gtcagggccg
    11821 cgacggtagg agaggtgttt cagccaggcg gaatcgtcct gttgcctgtg gtcgcggcgc
    11881 tgatgtgctc cgcggctctc ggtacgggcc agggcggcac gcaggatcag gcgagcgatc
    11941 agcagcatgt tgcgggcttc gagcgcggct acgctctccg aggtgctctc cacttttaga
    12001 ccggcgtcga cgtcggcctg caagcgatcg gcatcggcaa tgcctccagc gagcccggtt
    12061 gcgtctcgat aaaggcctgc cgcacgggcc atcagttgcc ggacggcacg cttgatctcg
    12121 ataggctccg cgctgccgtg ggttcgtcct gccgcgcgaa gagaatcgac ggccatacgc
    12181 tcgatccgcg accagtcccg ggcggctggc gccagcagtc cggaagccgc tccgcggccg
    12241 gcgattgccc cgaagatgat cgtttcgctg ccgccgttgc cggcaagacg gctcgcgccg
    12301 tgcacgccac cggccgcttc cccgcaggca tagagaccgg gaacacctga ccagccatgg
    12361 tgatcaatgt gcacgccacc catctggctg tgcgcggcag gacggacccg tggcccctcg
    12421 acagccggat cgacgcccgc tgcccgcaga cgcttgcaat gcgaaacgta gctctccagg
    12481 cggtcgcggg ggacgctcgt cgtatcgaag agaaccgtgc cgtcgggaaa gccccggcct
    12541 tcatcgatct cctgttggat gcacagcgac atgcgagcct tctcgatccg cttttcgccg
    12601 tgctccgggt tgtagcgaaa catgaaccgc tcgcccttgg cgttgatcag gcgagcgccg
    12661 tcgcccagca tagccgtcgg catctccaga ccacggcatt cctccggcca cactgtcacg
    12721 accggctcga actgtacgaa ctccatgtcc acaagcctcg ccccgacatc gtaggcgagg
    12781 gcgtatgaat ccgcggcgat gtcggcagga taagtgctgt cgtcatagat gcggccaatg
    12841 cctcccatgg cgaggatcgt cgcccgcgcc cgaatggcca gcagctcgcc cgtatggcgc
    12901 ttgaccagca gtgcgccgac aacctcaccc tgatccgaga gcagggagac gactttccag
    12961 ccctgacacg tcgcaacccc gatagctttg gcgcgcgctt tcaggtagtc gagaatcacc
    13021 ctgccggtgc cctcggggac gaagaccgag cgcggatagg tattgcccga aagatggcgc
    13081 tgcagaacac gcccgttgtc gcgggcgaac ggcacgccga gcccatcgag ctcccggaat
    13141 gatggcacgg cctgggctgc catcgcttgg acgaggcgac gatcgttcag cccatatccg
    13201 ccgcggacca tgtcgtcgaa gtagacctgc ggcccgtcgg ccggatcgga cgccccgagg
    13261 ggaatgttgg cgccgatcac gtagggtgat gctccgtggg cgagactggc caggacgacg
    13321 ctgcaatcgg cctcgcgcgc ggcgacggcg gcacggtagc cggcaatgcc gccccctagc
    13381 acgaggacgt cagtcgtcat cacgctttcg atttggctcg aggtgacacc catcgcagcc
    13441 tactcgcttg cgctggcggc ttgcggcgtt tgcggcgcgg gcctccagat ccaccacaag
    13501 agcagcgcca ctgcaatgag cccgaatccg atcaaatccg tcagaatgcc gggcttgatc
    13561 agcgctagcg cgcccccgcc gagaagcagg cgctgcagcc agttggcagg tccgcccaga
    13621 agatagccct gaaccgctgc agcaagggcg atgattccga ccagactcgt cacgaccgca
    13681 agagcgatga cgaacggcgt gccttgaagg agcagggctg gattgtagac ccagatgtag
    13741 ggcagcagaa atccagcgag cgccagcttg aaggcgatca cccccgtcgt cgtcgcgttc
    13801 gctcccgcga ggccggctcc ggcataagcc gcgatcgcga cgggcggcgt cacgttggcc
    13861 agaacgccga agtagaagac gaacatgtgc gcagcgagcg ggttgacgcc catctcgacc
    13921 atggccggca cgacgagggt cgcgaggatg atgtagacgg ccgaagtggg caggcccatg
    13981 ccgaggatca gcgagatgat catggtaatg atcatcagca cgaagaggct gccgccggcg
    14041 aggtcgatca tgatgctgga gaggcgggtc gcgaggcctg tcagcatcag tattccgatg
    14101 ataattccgg cgcaggcgca ggctgcggcg acctcgagca ttccggtgcc gcttgcgcgc
    14161 agtgctgtcc agatgcggtc gggatacatg cgcgttgcgc cgcgcaggaa gctcactgcg
    14221 agaacggcaa cgatgccata gaaggcagag gtcacggggg tatattcgat cacgacgagc
    14281 aggtagacga gcaggacgag cggggcgagc aggtgccagc cacccttgaa tatcccccag
    14341 gcgctcggca gctcgcttcg cggcagcccc ttgatgcccg ccttggcgac ctcgaaatca
    14401 accatggtca ggacggccag gaagtagagg atgccgggaa agatggcggc cgtcatgatc
    14461 tcagagtagg gagcgccaag aaactcgacc atgatgaacg cggccgctcc catgacggga
    14521 ggcataatct gtccgccgga tgaggctgcc gcctcgaccg cgccggcaaa cgcgggcctg
    14581 aggccggcgc gtttcatgac ggggatcgtg aatgtgcccg tcgctacgac attggcgact
    14641 gccgatcccg agatggtgcc catcatggcg ctgccgacga ccgccacttt cgcagggccg
    14701 ccccgcatcc agcccagtcc ggcgtagctc cagtcgatga agaacttgcc ggcaccggag
    14761 gtgtcgagga aaccggcgaa gatgatgaag atggcgacat acgtggcaga gacctgcagc
    14821 ggtgttcccg caagtcccga tgtcgtcaga tagagagtgg aggcgatccg ctcgagatca
    14881 tagccccggt gcccgagcgc acctggcatg taaggcccga cgaaaccgta gatgaggaag
    14941 aggatggcca ggatcggcag cgcccatccg acgacgcgtc gcgtcgcatc gatgacgaga
    15001 atgacagcca ggacgccgag ccacactccg acctcagttg gatcgcccgc gccgcgacgc
    15061 gaaatctcca ggaagttgat gactgcatag gcgctgatgg cgactgatcc tagaatcagc
    15121 agagaatcga tcgaccatcg cagtaatgtg cgcgccttgg agccctcggc aacgaagggg
    15181 cgtgtcagga atatcaggac gagggcgaaa cccaggtggg cataggcctg ctgcatggtc
    15241 ggcagcgtgc ccgcatagct cgtccacaag tg
//
LOCUS       DASVDU010000237        29049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_433043, whole genome shotgun sequence.
ACCESSION   DASVDU010000237 DASVDU010000000
VERSION     DASVDU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 29049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29049
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433043"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF226_15195"
     CDS             complement(<1..612)
                     /locus_tag="VF226_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX7075384.1"
                     /translation="MLIGVPKEIKVREYRVGLTPTSVRELTALGHEVLVEKSAGAGIG
                     IDDSDYQRAGAAIVPQASDIYSHAEMIVKVKEPQPVERRMLRPGQILFTYLHLAPDPD
                     QTSDLLQSGAVCIAYETVTSPAGGLPLLAPMSEVAGRMSVQAGAYFLEKAHGGHGVLL
                     GGVPGVDPAKVVVLGGGVVGTNAVHIALGMGADVWVIDRNVDVL"
     gene            complement(652..2553)
                     /locus_tag="VF226_15200"
     CDS             complement(652..2553)
                     /locus_tag="VF226_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7075385.1"
                     /translation="MSADTESLRLKAILDSAVAGVITIDSTGRIETVNRAAERLFGYD
                     AEELAGRNVSMLMPEPYASKHDGYIRRYLETGERRIIGIGREVQGLRRDGTLFPMHLS
                     IGEYSIGNQRYFTGIIHDLTAQRAIEDRLDREQALFRSIFENLPDAVVISDPEGRIRL
                     VNPAFTQMFGYSQEEAVGQGWASLYADPQQAEPFIKSASGGGNSRGVQTIEFRRSGGK
                     LFPARAVHRAIIDRNGNTVGGLALIHDITAERAQAATLQQAQRMEAVGQLTGGVAHDF
                     NNLLTVILGNLELLEPKLKDEFCINLATEAREAAEMGARLTDRLLTFARRQRLETRRI
                     DLNEFVLSLTELLRRTLGTNIDLSTSMAADLWSTEADPGQIENAVLNLAINARDAMPN
                     GGRLVIETQNASLDAEAVEMIPGLAPGDYVILSVADTGVGMTPEVKARCFEPFFTTKG
                     ASKGSGLGLSTIYGFARQSGGNVTIYSEPGRGTTVNIYLPRATDAPTEEPRPASASVK
                     ASDGETILVVEDDDRVRRLTCARLKDLGYSVEEAANGQAALAVLESKPGIDLVFSDQV
                     MPGGLSGLDLAEAIRERYPKTRVVLTSGYSADLIGRERVDKLKLRVLRKPYQQADLAR
                     ILREALDGD"
     gene            2930..3370
                     /locus_tag="VF226_15205"
     CDS             2930..3370
                     /locus_tag="VF226_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7075386.1"
                     /translation="MYKHILLPTDGSEFSERAALYGVKLAQALNARVTAITVTAPWQA
                     IAIGEIAIAIPEGDYEMRSETNAWNFLNKITDAAKAAGVPRNAVHIRHPSPYEAIIEA
                     ANSHGCDLIVMGSHGRRGIAGLLLGSETVRVLTHSKIPVLVYRE"
     gene            3590..4564
                     /locus_tag="VF226_15210"
     CDS             3590..4564
                     /locus_tag="VF226_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7075387.1"
                     /translation="MFRRGLFIPVMLAALSAAAAAVVLSPGLLNFASGPNLTPGPQPA
                     AHSATPDTAAADLDALKPRDGSATSPAFDVARISPDGWSVFAGTAPPNSHVTVTADGE
                     PVGTAQADERGEWSLVTDHEFASKDPILGLSASPGEPAQQMAGAGYRQPEAKAAAGGL
                     DPSASMMQNFESLVAAARDPEQPSRSQAAMHAPLPVPIQFVFREATFTENGRKAMELL
                     LEYLKIKQPESVVLTGHADERGSDSFNLELSRERLDAVVKYLREHGFTGNLVLIPKGS
                     SEPYRGIDRSSLDRETLWQLDRRVELRLDADVPPHRSAQDSGRSNVAK"
     gene            4757..5470
                     /locus_tag="VF226_15215"
     CDS             4757..5470
                     /locus_tag="VF226_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075388.1"
                     /translation="MLLFAICPAVASAEPNSAVTASPAPVEPAVEAPEKANLTVQTFL
                     DRLMMAESGGLPNAKNPRSTALGPFQFIESTFLEVARRYFADEVAGLSDAEILAKRTD
                     FEFSRRAALAYTRENAAHLGSQGLEVTSVALRLAFLLGPAGATRLLRADEDTPVSAIL
                     SSEVLAANPFMASMTAADLLRKAALDISGNGRITGLGTGAEKPKIVIRCNLGLASCRR
                     WVALRKRMLARQQRWSQTQ"
     gene            complement(5497..6351)
                     /locus_tag="VF226_15220"
     CDS             complement(5497..6351)
                     /locus_tag="VF226_15220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7075389.1"
                     /translation="MRAAAVLALALSAVLAGTAAHADCPGNPHALGVSRTVEIDTTGG
                     PGFGFAQYRMYDFLMPKEVVLTFDDGPWPENTPAVLDALDEQCVKATFFIIGKHALWH
                     PEILKDVAARGHTIGFHTWSHANLRKMKSEKAKEEIEKGLSAVKLALGADPAPFFRFP
                     YLQDPKELVDYLGSRNIAMFSHDLDSFDFKLRKPDSVIKSVMQKLEKKGKGIILMHDF
                     QKVTAHALPKLLKELAEKGYKVVHMTAKAPATTLPEFDQLAKAEIKGPEGTGKPVSSV
                     VRTISTGQ"
     gene            6513..7430
                     /locus_tag="VF226_15225"
     CDS             6513..7430
                     /locus_tag="VF226_15225"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075390.1"
                     /translation="MRVRPAQLGGAMTNPVVGFIGLGLMGTGFTRRLIATGHRVIGYD
                     ISPEKVDAAAAYGVEKAASPAELADAADVVLACVTTTAAVAEVVEGPQGVLSASNLRG
                     KVFVDHSTTEIETTKRLAALLAERAGMGMVDAPVSGGPGAAEAGTLAIMAGGTDEAIA
                     SVAPLMGQLGTFTHMGGPGAGQATKLVNQTLVLTNYCVIAEALRLAETYGIDASKLPQ
                     ALGGGYAGSNLLPVMLERMIPRDWTPKGYARQVLKDLEMVNEAARSRHLAMPMTSQAL
                     TLFRMLVAQGKSEIDGAAVLEVLPPGDKP"
     gene            complement(7506..7829)
                     /locus_tag="VF226_15230"
     CDS             complement(7506..7829)
                     /locus_tag="VF226_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEX7075391.1"
                     /translation="MTTFEDREKAFEKKFAHDQELKFKAEARRNKMLGEWAAGLLGMS
                     GDEVEDYVKSVRRADLAEKGDEDVFLKLRGDFDAKGVSISDAELRAKMSEFLAEAVKQ
                     LEIPK"
     gene            8169..8957
                     /gene="purC"
                     /locus_tag="VF226_15235"
     CDS             8169..8957
                     /gene="purC"
                     /locus_tag="VF226_15235"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX7075392.1"
                     /translation="MNRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNNKKHALIE
                     GKGVLNNRISEYIFVKLGEIGVPTHFIRRLNMREQLIREVEIIPLEVVVRNVAAGSLA
                     QRLGLEEGTQLPRSIIEFYYKADNLNDPLVSEEHITAFGWATPHEIDEIMQLALRIND
                     FLVGLFLGIGIRLVDFKVEFGRLWDNDQMRIVLADEISPDCCRLWDVQTSDKLDKDRF
                     RRDMGGLLEAYQEVARRLGVFTDNRPAKGAVPVLVASNPGGKPN"
     gene            9086..9328
                     /gene="purS"
                     /locus_tag="VF226_15240"
     CDS             9086..9328
                     /gene="purS"
                     /locus_tag="VF226_15240"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX7075393.1"
                     /translation="MKARIIITLKNGVLDPQGKAIQNAVGALGIEGVQSVRQGKYIEV
                     ELAATSEAAARETVDRMCRELLANTVIENYTYEIEG"
     gene            9594..10289
                     /gene="purQ"
                     /locus_tag="VF226_15245"
     CDS             9594..10289
                     /gene="purQ"
                     /locus_tag="VF226_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX7075394.1"
                     /translation="MKASVIIFPGTNRERDVADVLEAVSGRAAQMVWHGDTEVPASDL
                     IVLPGGFSYGDYLRCGAMAAHSPIMRDVVAKAKAGTPVLGICNGFQILCEAGLLPGVL
                     MHNASLRFVCKEVWLRVEQTDTLFTSRFAKGEVMPAIAAHAEGNYFADTDTLDRLEGE
                     GRVVFRYADRHGNVTPEANPNGAQRSIAGICDARRRVLGLMPHPENTSDPLLGPTGGR
                     KLFESIVDNLVAA"
     gene            complement(10319..11269)
                     /locus_tag="VF226_15250"
     CDS             complement(10319..11269)
                     /locus_tag="VF226_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009807491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7075395.1"
                     /translation="MARHSHPHSHGHVHIGAEAGDRRLWWAVLVNVALTFVQVVAGIL
                     AGSLALVADGVHNLSDAVSLGIAYGARRIARRPSDELMTFGYVRAESIAALINYTTLI
                     VIGVYLAYEAVARFFAPAEVEGWIVVWVAGVALAIDAVTALLTFSLAKTSQNVRAAFL
                     HNLADALGSVAVIVAGALILTFGWYIVDPIVTIGISAYILWMALTEIGSVIRLLMLGV
                     PPEISVDELVDAIRKVDGVENVHHVHVWAIDEHRTSLEAHIVVPDALASDPKPIKDRI
                     RELVRDRFGVSHMTLEVERASECHEGEHANIIGHQIGARH"
     gene            complement(11364..13022)
                     /gene="ettA"
                     /locus_tag="VF226_15255"
     CDS             complement(11364..13022)
                     /gene="ettA"
                     /locus_tag="VF226_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX7075396.1"
                     /translation="MATPQYVYHMHRLSKTYPGGKQVLKDISLSFLPGAKIGVVGLNG
                     AGKSTLLKIMAGEIEDYQGEAWAADGVRVGYLPQEPELDPDKDVLGNAMEGVAEKKAL
                     LDRYNELAANYSDETADEMARLQDEIDAQNLWDLDQQVEQALDALRCPPGDADVTNLS
                     GGEKRRVALCRLLLSKPDILLLDEPTNHLDAETVAWLERYLREYPGCIMLVTHDRYFL
                     DNITEWTLELDRGQGVPYKGNYSSWLEQKAKRLEVEKGQEEAKQRALKRELEWIRSSP
                     KARQAKSKARIQAYERLAEEAGREQIGHAQIQIPPGPRLGSVVLEVEGLVKGFGDRLL
                     IDNLSFKLPPGGIVGVIGPNGAGKTTLFRMITGQEQPDAGTIRLGDTVKLGYVDQSRE
                     DLDPKKTVWEEISGGNDMIQLGKREVPSRAYVGWFNFRGPDQQKKVGMLSGGERNRVH
                     LAKMLKEGSNLLLLDEPTNDLDTETLSALEAALEDFPGCAVVISHDRFFLDRIATHIL
                     AFEGDSHVEWFEGNFEAYEEDKKRRLGEAAVEPHRIKFKRFERS"
     gene            13289..13921
                     /locus_tag="VF226_15260"
     CDS             13289..13921
                     /locus_tag="VF226_15260"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7075397.1"
                     /translation="MTLELVYFADPMCSWCYGFAPVVAAIEQHFRGRLPLRLVMGGLR
                     AGQTQPMREQDKEHVRGEWARVSEATGQPFDFAFFDRDAFVYDTEPACRAVVAMRTLD
                     PSRARAFMSRIQGAFYAENRDVTSTEVLADLAAEEGQNRDAFLATFLSPEVRNETFRD
                     FLIAQELGIHGFPTLIVGGGRQYTLVTNGYRPLDGIPEALDQWLARQPEA"
     gene            complement(14045..14758)
                     /locus_tag="VF226_15265"
     CDS             complement(14045..14758)
                     /locus_tag="VF226_15265"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7075398.1"
                     /translation="MKAMLGLSAAGTLPRDMAIVLPGLARRVRLAFEIALFFVAAPIL
                     VSLVLKTFRLPLFLVLQPILLGFFIYLLWDPTFLLRRELARGFSLRDLLAIIGTFLVL
                     GSAIAYAAHILHPSGFFSFPYRLPRLWLTIMVLYPLMSVLAQEFVYRTFFFHRYGPLF
                     GNARWAAIVANAALFGFAHIIFNNLIAVAGSFFIGLLFAYRYDVTRSLWAVWLEHSLY
                     GCLVFTVGLGRYFFTGISI"
     gene            complement(14766..15386)
                     /locus_tag="VF226_15270"
     CDS             complement(14766..15386)
                     /locus_tag="VF226_15270"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX7075399.1"
                     /translation="MHLNLERLRAAKVENDPYEYVIVPGFLSMESLRRINDTYPRIER
                     GGSFPIESLEPGMTIKEVIEELDGPEFQAIIEEKFGVDLENRPKMYSLRGYTRSKDGR
                     IHTDSPDKIITVLLYLNETWPHASGRLRILRNGNDVDDFVAEVPPDQGTLLVFKRSDN
                     SWHGHHPFEGPRRSLQMNWMTSESSKGFHKMRHTISATFKKLIAAE"
     gene            complement(15429..16478)
                     /locus_tag="VF226_15275"
     CDS             complement(15429..16478)
                     /locus_tag="VF226_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEX7075400.1"
                     /translation="MQQQDLSVGNAPSVGALSGARAWIITDGKAGMVVQCKGVADALG
                     LDYEMKRVTTRGFWRLIPPWGPVQPVDRPGRPGALLSPPWPAIAIGSGRASIPYIRAI
                     RRYSRGATFTIILQDPRTGPGVADLIWVPEHDRLRGPNVLVTPTSPHSHTPERLAKLR
                     GELPPEIASLPHPRITVVLGGKNASYKFTDADDDRFAAALVSLGRLGASFMITPSRRT
                     HNRLIRIADEATRNSPRIFWDGTGPNPYPYFLAAADYLVVTADSVNMTGEACATGRPV
                     YVFEPSRGSAKFRRFHQRLQGIGATRPLPVEVQRLEDWTYAPIDSAATIAREVERRYL
                     LRSAELTDKGGSVGR"
     gene            16660..17139
                     /locus_tag="VF226_15280"
     CDS             16660..17139
                     /locus_tag="VF226_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7075401.1"
                     /translation="MRARLDVIDWRILKELQADGRITNVALAQRVGISAPPCLRRVRA
                     LEEAGFITGYHAHVDEKMLGFELTAFAMVGLHSQAEPDLREFENRVLGWPLVREAYMM
                     SGETDYVLKCVAPDLTTFQNFVIRELTAAPNVASVKTALAIRRAKLEPGVPIGYAQS"
     gene            17375..17836
                     /locus_tag="VF226_15285"
     CDS             17375..17836
                     /locus_tag="VF226_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7075402.1"
                     /translation="MAEAPSPDIRKPNLVGINHVALEVGSIDEALAFYGSIFSFKLRG
                     RGKSSAFIDMGDQFIALMESDTPAGDKERHFGLVVDDRSAVRQLAEAAGAKVLPGHFL
                     DFLDPWGNRVQVVEYSDIQFTKAPDVLKAMGLDLRKSDKALRELADKGIIF"
     gene            17859..18317
                     /locus_tag="VF226_15290"
     CDS             17859..18317
                     /locus_tag="VF226_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX7075403.1"
                     /translation="MPKRSAGLLLYRLTGGEVEVFLVHPGGPYWAKKNEGAWSIPKGE
                     IDEGEDPLSAARREFKEETGVWPEGEAIPLGTVRQPGGKLVTAFAVEGDLDADTIRSN
                     TFTMEWPPRSGRMRAFPEVDRAGWFGKEEAERKILKGQRAILDALWKMVT"
     gene            18461..18952
                     /locus_tag="VF226_15295"
     CDS             18461..18952
                     /locus_tag="VF226_15295"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7075404.1"
                     /translation="MSQLTGIAIALTLLLAGASPSVAQSESEREAVRAANQAYYDALS
                     ARDIQAMEQVWSREADDVLVAPPIRPTAHVGWDKIKKQYETFWATLEELTVSMEQPTI
                     HIEGNVAWTFGTEHAKRRTKDGQASGGPNFGTNIFVKRDGRWLLVFHQAALMPQPPAE
                     KAK"
     gene            19031..19441
                     /locus_tag="VF226_15300"
     CDS             19031..19441
                     /locus_tag="VF226_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7075405.1"
                     /translation="MIDLAVLHPGLTGTSTLRVAPEHTAPHVGSGRVAVLATPVMINV
                     IEAAALNAVEHLLPEGHQSLGIHLDVRHFAATPVGLLVTATAEVIAVEGRTITFRVEA
                     RDECEAIGEGTHQRVIVNVARFDERVQRKLKSFG"
     gene            complement(19520..20152)
                     /locus_tag="VF226_15305"
     CDS             complement(19520..20152)
                     /locus_tag="VF226_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX7075406.1"
                     /translation="MSKLTLYHAAPSRSSVVLWMLEELGQPYDVQLISFKKGENLQPE
                     FLAINPMGKVPALRHGDAVITESAAICTYLADEFPQAKLNIPTGDPRRGPYLKWLFFG
                     PSCLEPAITDRAFPRKEEPPRATLGFGNFDLVMDVLAKATAEADPYLLGSQFTAADVV
                     IGSGLNWAMMIKLIPERPEFVAYVDRLNARPAAQRAQAKDAEYKKQQEGA"
     gene            complement(20251..20688)
                     /locus_tag="VF226_15310"
     CDS             complement(20251..20688)
                     /locus_tag="VF226_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7075407.1"
                     /translation="MYKNILVTTDGSEFSEKAFGHAIELAKLVGARLTAVTVTPPLQL
                     LTVEGVVYPEDPEKYQQSIDAQAKAALNVVAKAAEARGVPCELVQLESPQPYEGIIKT
                     ANDKGCDLIVMASHGRRGLSAFLLGSETQKVLTHSNIPVLVCR"
     gene            complement(20811..21140)
                     /locus_tag="VF226_15315"
     CDS             complement(20811..21140)
                     /locus_tag="VF226_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7075408.1"
                     /translation="MTSPALFDIAEFEAGAAEVARVLRALSNERRLMILCKLLEWGEA
                     NVTSLGEAVGLSQSALSQHLAIMREQNLLAYRREGQTLWYRIADPRIERLLATLYDLY
                     CPSSKAN"
     gene            complement(21326..21694)
                     /locus_tag="VF226_15320"
     CDS             complement(21326..21694)
                     /locus_tag="VF226_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7075409.1"
                     /translation="MTDTTVKKVHAPDSPKGDMGQKYLVSGKRVSMRLWEHEPPGSRK
                     DESVREYETVGYVIDGRAELELEGQTVKLEPGDSWLVPAGAKHSYKILEDFTAVEATA
                     PPSQVHGRDEPRGSDWPERI"
     gene            complement(21815..22708)
                     /gene="mmsB"
                     /locus_tag="VF226_15325"
     CDS             complement(21815..22708)
                     /gene="mmsB"
                     /locus_tag="VF226_15325"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEX7075410.1"
                     /translation="MTKIAFIGLGNMGTPMAGNLVKAGHTVVGYDLVPANLERAAERG
                     VTPAQSADAAAEGAEVVITMLPAGKDTIAVYEQTGVLSAAASGAVLVDCSTIDVASAR
                     RAHELAGNAGKLSLDCPVSGGVGGAEAATLTLMAGGSTEAFEKARPVLEKVSRKVVHC
                     GDAGAGQAAKICNNMILGISMIGVCEAFNLAEKLGLSKQALFDVASTSSGQCWSLTTY
                     CPAPGPVPTSPANNGYKPGFAAALMLKDLSLAQEAARAASVATPLGAEAAQLYQLFVA
                     AGNAGVDFSGIIEFLRGKADK"
     gene            complement(22746..23549)
                     /locus_tag="VF226_15330"
     CDS             complement(22746..23549)
                     /locus_tag="VF226_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7075411.1"
                     /translation="MDRAQTAASAQEIIYEVRDRIGFLTLNRPHARNALTFGMYDRIA
                     EICANAPTDGSLKAIIITGAGDRAFASGTDISLFRDFGTPEQGIQYEKHADEIMTAIE
                     SCPVPTIAAIAGACTGGGAGIAACCDMRIATRDLKFGFPIARTLGNCLSAATLERLVR
                     LLGEPRVKDMIFTARLVGAEEALTAGLVSEVVESHAALMTRAEELARQIAGYAPLTLR
                     VTKEILLRLRQQGPKVDDTDLVAKVYTSADFREGLEAFLAKRPPQWTGR"
     gene            23809..24330
                     /locus_tag="VF226_15335"
     CDS             23809..24330
                     /locus_tag="VF226_15335"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7075412.1"
                     /translation="MRSTPHDPPSAVSRRLPIRSPQDFAGGMLLLAIAVLGFLGSWHL
                     DFGQFGGVGSGMVPQAVSAMIAGFGLLLIVQGFVSSDGAQMTLWSIRGLFFVLGATLL
                     FAWTIRPLGLIFAGPIAIVFASLADRTTRPLEIAIFTVALTAFCIGLFSFLLQLPIPV
                     LPSSLPYPFNEIF"
     gene            24340..25842
                     /locus_tag="VF226_15340"
     CDS             24340..25842
                     /locus_tag="VF226_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7075413.1"
                     /translation="MTELTDHLALGFATALTLKNLGLCFLGCMIGSLIGVLPGVGPIA
                     TIAMLLPLTFGVDPVGALIMLAGIYYGAQYGGSTTAILVNIPGEATSVVTVLDGHQMA
                     KQGRAGIALGICAIGSFIAGTVATLIIAALGTPLTKMALLFGPADYFSLMVLGLIFAV
                     VLASGSVPKAIAIILVGLLMSTIGIDIETGEERLTFGFNELADGIPFAVAAMGIFGFA
                     EIIRNLEVAETRDVVRAPIGRLLPGLRELRQSALPIARGTLVGSILGLLPGNGAVLGP
                     FASYTLEKKLAAEPERFGQGAIEGVAGPESANNAGAQTSFIPLLTLGIPPNAVMALMV
                     GAMTIHGIVPGPQVIQHQPALFWGMIASMWIGNLMLLIINLPLIGVWVRLLKVPYRLM
                     FPAIVVFCCIGIYSMNNSGLQVFMVAVFGLFGYMLVKFGFEPAPLLLGFVLGQLMEEK
                     LRQALVISRGSFITFFDRPVSALLLALALALVVVAVLPSIRSRRDTVFQE"
     gene            26082..26237
                     /locus_tag="VF226_15345"
     CDS             26082..26237
                     /locus_tag="VF226_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075414.1"
                     /translation="MAINRLRHRSREVVIALAITLAAIAAVIATLSSQEEDAQTAEPM
                     ALVTPRK"
     gene            complement(26275..27054)
                     /locus_tag="VF226_15350"
     CDS             complement(26275..27054)
                     /locus_tag="VF226_15350"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX7075415.1"
                     /translation="MQLKGKSAIVTGSTSGIGLAIAHAFAKAGANVMMNGLGDENENA
                     NQIREVAAHGTRVAFHPANMLRHNQIADMVAEAERTFGQVDILVNNAGIQHVSPIEEF
                     PIEKWDAIIAINLSAAFHAIRAVVPGMKARRWGRIINICSAHSLRASVNKSAYVAAKH
                     GLAGLTKVVALETAEHGVTANAISPGFVWTPLVEKQIPDLARTQGLTEEEARAQILAR
                     QPTREFVTVDQVGALAAFLASDAAASITGANYSIDGGWTAQ"
     gene            complement(27134..28633)
                     /locus_tag="VF226_15355"
     CDS             complement(27134..28633)
                     /locus_tag="VF226_15355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7075416.1"
                     /translation="MTVHAARGSDAGVRAGWLPALLAVLGLLLAIRLVALAFNGTDLF
                     FDEAQYWSWGLEPAFGYYSKPPLIAWIIRAATETCGSSEFCVRLPSPIIHTATALVIY
                     ALTARLYDRRAGFWSAVVFATLPGVSLSAGIISTDVPLLFFWAVALYALVRLLETDRW
                     WPALLLGLAFGLGLNAKYAMAFFVFSAAAFMLVAPQGRCLLRDARLYAALLIGLVLIA
                     PNLAWNIENGFATLAHTADNAKWTGVLFHPNKALEFFGAQFGVFGPILFGSLLVITWR
                     AWLEGLPKPDQLMLAFAVPVILLVTVQGLLSRAHANWAAVSYVSATVLVTTTMLREGA
                     WRWLKGSLALHAAILVGIGIATAKAGSFRLPGGGDPFARTLGWRTLAEETAAVLDEAR
                     RAGRPFGAVLTDDRAVTAELLYYMRDEPTPVFAWREPGVRPRDHYELTRAYRKGAPEP
                     VLLVTLRPDAGELSDRFAIAEILTERRVPAGAGPARRVTFYRLDGFRLE"
     gene            28780..>29049
                     /locus_tag="VF226_15360"
     CDS             28780..>29049
                     /locus_tag="VF226_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7075417.1"
                     /translation="MRISVVIPALNEAGNIGRLVQETYATVPADTLAEVIVVDDASTD
                     ATGAEVKALIQSNDFPGLRYLRHGQRAGQSAAMRTGILAATSPVIA"
BASE COUNT         5260 a         9377 c         9061 g         5351 t
ORIGIN      
        1 caggacatcg acgttgcggt ctatcaccca gacatcggca cccatgccga gggctatgtg
       61 gacggcgttg gtgccgacca ccccgccgcc gagcacgacc accttcgcgg gatcgacgcc
      121 cggcacaccg ccgagcagca cgccgtgccc gccatgcgcc ttctcgagaa aataggcgcc
      181 ggcctgcacc gacatgcgcc cggccacctc cgacatcggc gcgagcagag gcagaccgcc
      241 ggcgggagac gtcaccgttt cataggcgat gcaaacagcg ccgctttgaa gcaagtctga
      301 agtctgatcg gggtcgggcg caaggtgcag gtacgtaaac aggatctgcc ccggccgcag
      361 catccttcgc tccaccggct gaggctcttt caccttcacg atcatctcag catgagagta
      421 gatgtcgctc gcctgtggaa cgatggcagc acccgcgcgc tggtagtcgc tgtcgtctat
      481 gccgatgccg gcgccggcgc tcttttcgac aagcacctcg tgcccgagcg ccgtcaactc
      541 gcgaacgctg gtgggcgtca gcccgacccg atactcgcga accttgatct cctttggcac
      601 cccgatgagc atgcttggac ctgccaatgg tcctggagat cgaacgcagc gctaatcgcc
      661 atctagagct tcgcgcagga tacgggcaag atcggcctgt tgatagggtt tgcggagcac
      721 cctgagcttg agcttgtcga cccgctcgcg tcctatcaaa tcggcggaat agccggatgt
      781 cagcacgacg cgcgttttgg gataccgctc ccgaattgcc tcggcgaggt cgagtcccga
      841 caagccaccc ggcatgacct gatcggagaa gacgagatcg atgcccggtt tgctctcgag
      901 aacagccagg gccgcctgtc catttgcagc ttcctcgacg gaatacccga ggtccttgag
      961 ccgggcgcag gtgagccggc gtacgcggtc gtcgtcctcg acaacgagga tggtctcgcc
     1021 gtcacttgcc ttgaccgaag ccgatgccgg tcgcggttct tccgtcggag catcagtggc
     1081 gcgcggcagg taaatattaa ccgtggtccc gcgacccggc tcactgtaga tcgtgacgtt
     1141 gccgcccgac tggcgcgcaa agccgtagat ggtcgacaac ccaagcccgc tgcccttcga
     1201 agctcccttg gtcgtgaaga acggctcgaa gcagcgcgcc ttgacctcgg gggtcatgcc
     1261 gacgccggta tcggcaacgg acagaatgac gtagtcaccc ggcgcgagtc ccgggatcat
     1321 ctccaccgct tcggcgtcga gagatgcatt ctgggtttcg atcaccagcc tgccgccgtt
     1381 cggcatggcg tcgcgcgcgt tgatcgccag attgagcacc gcattctcga tctggccggg
     1441 gtcggcttcc gtcgaccaga gatcagccgc catagaggtg gacaggtcga tgttcgtccc
     1501 aagcgtccgc cgcaaaagct cggtcaggct caacacgaac tcgttgaggt cgatgcggcg
     1561 cgtctccagg cgctgccgac gggcgaacgt cagcagccgg tccgtgagcc gcgcccccat
     1621 ttccgcggcc tcgcgcgctt ccgtggccag gttgatgcag aactcgtctt taagcttcgg
     1681 ctcgagcagc tcgaggttgc ccagaatgac cgtcagcagg ttattgaagt cgtgggccac
     1741 gccgcccgta agctgaccca cggcctccat gcgctgagcc tgttgcagcg ttgccgcctg
     1801 ggcgcgttct gcggtgatgt catgaataag ggctaggccg ccgacggtat tgccattgcg
     1861 gtcgatgatt gcgcgatgga cggcccgtgc cggaaacagc ttaccgccgc ttcgacggaa
     1921 ctcgatggtc tgtacaccgc ggctatttcc gccacccgac gcagatttga tgaacggctc
     1981 ggcctgctgg ggatcggcgt acaggctggc ccagccttgg cctacggctt cctcttgcga
     2041 atagccgaac atctgcgtga aagccgggtt gacgagccgg atccggcctt ctggatcgga
     2101 tatgacgacc gcatccggga gattctcgaa gatcgagcgg aacagagcct gctcccggtc
     2161 cagccgatcc tcgatcgccc gctgagcggt gagatcgtga atgataccgg tgaaatagcg
     2221 ctggtttccg atcgagtact cgccgatcga caggtgcatg ggaaagaggg tgccgtcccg
     2281 gcgcagccct tgcacctccc ggccgatccc gatgatcctg cgctccccgg tctccagata
     2341 tcgccgaatg tatccgtcgt gcttgctcgc atagggctcc ggcatcaaca tggagacgtt
     2401 gcggccagcg agctcctctg cgtcgtagcc gaacagcctc tcggcggctc gattgacggt
     2461 ctcgatccgc ccggtgctgt cgatcgtgat gacccctgct acggccgagt cgagaatggc
     2521 cttcagccgc agcgattccg tgtcggcgct cacgcgccct gtcgccgtca tggacgtgcc
     2581 gatctgagtt tcacgcgcct tagttggcgc actagcatgt cataagtcga gcagacgaga
     2641 gttaacgtga caatgagcct cagaagcgga tttcagaggc cgaagcgaaa gcattcgcgc
     2701 tctctaacga aaaatgtgaa gagtggctgg cccggactcc ttgatgtcga tcaatgccga
     2761 gagaccgctg gccgggacag gatgaggctc gtgaccgccc ttcaggttca accctggtaa
     2821 atctcgggcc ggtttgccgt gtttctgcga cgggcttgac cggaacagat accggcccaa
     2881 tgtgtgcaga agtcgcggcg ggcaagccaa tcaggacagg aggacctgcg tgtacaagca
     2941 cattcttctc cctaccgacg ggtcggaatt ttccgaacgg gcggcgctct acggggtgaa
     3001 gctcgcgcag gcgctcaacg cccgcgtcac agcgatcacg gtgaccgcgc cctggcaagc
     3061 catcgctatc ggcgagatcg ccatagcgat cccggagggc gactatgaga tgcgctcgga
     3121 gacgaacgcc tggaatttcc tgaacaaaat caccgatgcg gccaaagcgg ccggcgtgcc
     3181 ccggaacgcc gtccatattc gtcacccaag cccttacgaa gcgatcatcg aggcggcaaa
     3241 ctcgcatggc tgcgatctca tcgtcatggg ctcccacggc cgccgcggaa tagccggcct
     3301 gctgctcggc agcgaaaccg tgcgcgtgct gacccatagc aagatcccgg tgctcgtcta
     3361 tcgggagtaa tccaccaatc cgatcatcac gccggtttca ggccgagccg caccgggctc
     3421 ggccttttct ttttgttttc gccagccccc gacgacatgg ccagatgaac cgggcgatca
     3481 ctgaaccgtt aatcttaacc tctctgaatt ttcagaggaa aatcactgga acagcttgtt
     3541 tacgctgcgt tccggatgtt agaagggttg ctgtagaagg cgtacgcaga tgttccgtcg
     3601 cggtctgttc atacctgtga tgcttgccgc gctatctgct gcagcggcgg cagtagtgtt
     3661 gtcgcctggc ctcctgaatt tcgcgtctgg tccgaatctt acccctggcc cgcagcccgc
     3721 ggcccattcc gcgacgcccg ataccgctgc agccgatctc gatgcgttga agccgcgcga
     3781 cggatcggcg acctcccccg cattcgacgt cgcccgcatc agccccgacg gctggtccgt
     3841 gttcgcgggg actgcaccac ccaattcaca cgtcacggtc accgctgacg gcgagcctgt
     3901 cgggacggct caggcggacg agcggggcga atggagcttg gttacggacc acgaattcgc
     3961 cagcaaggat ccgatcctcg gcctttccgc gagtcctggc gaaccagcgc agcagatggc
     4021 gggcgcgggc tataggcagc ccgaggcgaa ggctgctgcc ggcgggctcg atccctcggc
     4081 cagcatgatg cagaatttcg agagcctcgt tgccgccgcg cgcgaccccg aacagccatc
     4141 gcgatcgcag gctgcaatgc atgcaccgct tccggtgccg atccagttcg tgttccgtga
     4201 ggccactttc accgagaatg gccgcaaggc gatggagctg ctgctggagt atttgaagat
     4261 caagcagccg gagtccgtcg tgcttaccgg ccatgccgac gagcgtggca gcgattcctt
     4321 caatctcgag ctgtcgcggg agcggctgga cgcggtcgtc aagtacctga gagaacacgg
     4381 ctttacgggc aatctagtgc tcatcccgaa gggtagctcc gaaccctacc gtgggatcga
     4441 ccgctcatcc ctcgacaggg aaacgctctg gcaactggat cgcagggtcg aactccggct
     4501 cgatgcggac gtccctccgc atcgatcggc acaggacagc gggcgatcaa acgtagcgaa
     4561 gtagcacgtc cgactttcgc cacctcaacc aaagcgcctt tcactcgatg agctccaacg
     4621 acggaacttt tcgtgtcgag caagcgttcg ctgcacgagc ttcgctgtgc aatcggctga
     4681 aatggatcgt accaggacca catcggccct tcgacccgat cacggactgc ttcagtggag
     4741 agcagcaatc gcggccatgc tgctattcgc gatttgtcct gcagtcgcct cagcggagcc
     4801 gaatagcgcg gtgacggcaa gcccggcacc ggtagagcct gccgtcgagg cgcctgaaaa
     4861 agccaatctc acggttcaga cgttcctcga ccggctgatg atggccgaat cgggcggtct
     4921 accgaacgcg aagaacccgc ggtcgacggc cctcggcccg ttccagttca tcgaatcgac
     4981 cttcctggag gtcgcgcggc gctactttgc cgatgaggtc gccggtctgt cggatgcgga
     5041 gatcctggcg aagcggaccg atttcgagtt ctcgcgccgg gccgcgctgg cctacacgcg
     5101 ggaaaacgcc gcccatctcg gcagccaagg tctggaagtt acatccgtcg cgctgcgcct
     5161 ggcttttctg ctcggcccgg ccggagcgac gcggctgctg cgcgcggatg aggatacacc
     5221 ggtttcggcc attctgagca gcgaagtgct cgcagccaac ccgttcatgg cctcgatgac
     5281 cgccgccgac ctccttcgca aggccgcgct ggacatcagc ggcaatggac gcataacggg
     5341 gctggggacg ggtgccgaga agccgaaaat cgtcatccgc tgcaatctgg gcctggcgag
     5401 ctgccggcgc tgggtggcgc tgcgcaagag gatgctcgcg cgacaacagc gctggtcaca
     5461 gacgcagtag gcatagagct cgctcctcgc ggccagctac tgccctgtgc tgatcgtacg
     5521 gaccacactc gagaccggct tgccggtgcc ttccggcccc ttgatttccg ccttcgcgag
     5581 ttgatcgaac tctggaagcg ttgtcgccgg cgccttcgcg gtcatgtgca cgaccttgta
     5641 tcccttctcc gccaactcct tcagcagctt gggcagcgcg tgcgccgtca ccttctggaa
     5701 gtcgtgcatc aaaatgatgc ccttgccctt cttctcgagc ttctgcatca cggatttgat
     5761 gaccgagtcc ggcttgcgaa gcttgaaatc gaagctgtcc aggtcatgcg agaacatcgc
     5821 gatgttgcgc gagccgaggt agtcgacgag ctccttggga tcctgcaggt aggggaagcg
     5881 gaagaagggt gccggatctg ccccgagggc gagcttcacg gcgctcaggc ccttctcgat
     5941 ctcctccttg gccttttcgc tcttcatctt gcgcaggttg gcgtgagacc acgtgtgaaa
     6001 gccgatcgtg tgcccgcgcg ccgcgacgtc cttcaggatc tcgggatgcc agagggcgtg
     6061 cttgccgatg atgaagaatg tcgccttcac gcattgctcg tccaaggcgt cgagcacggc
     6121 cggcgtattc tcaggccagg gaccatcgtc gaatgtcagc acgacttctt tcggcatcag
     6181 gaagtcgtac atgcgatact gcgcgaaccc gaagccaggc ccaccggtgg tgtcgatctc
     6241 caccgtccgc gagacgccga gcgcatgcgg attgcccggg cagtccgcat gagcggcagt
     6301 gcccgccagt acggctgaga gcgccagagc aagaacggct gcagctcgca ttcaattccc
     6361 ccgtgctttc gagctcagcc gagcccgctg tttcctttct ccctcgacgg acaatataag
     6421 ctgatcgagg ccccgctggc gagcctcggc gactgctatg gcgtgcaaaa gattacggcc
     6481 gctcgattct cccgctatgc tgggtagcag aaatcagagt tcgaccagcg caacttggag
     6541 gagcaatgac caatcccgtt gtcggcttca tcgggctggg gctgatgggc accggcttca
     6601 cgcgtcggct gatcgccaca ggccacaggg tcatcggcta cgacatctcg cccgagaagg
     6661 ttgacgctgc agcagcctac ggcgtggaga aggccgcctc gccggccgag ctggcagacg
     6721 cggccgacgt ggtgcttgcg tgcgtcacga cgacggcggc ggtcgccgag gtcgtggaag
     6781 gtccgcaggg agttctctcg gccagcaacc tgcgcgggaa ggttttcgtc gatcattcga
     6841 ccaccgagat cgagacgacg aagcggctcg ccgccctgct cgccgagcgc gccggcatgg
     6901 ggatggtgga cgcaccagtc tcgggcggcc ctggtgccgc ggaagccggc acgctcgcga
     6961 tcatggccgg cggcacggat gaggccattg ccagcgtggc gccgctgatg ggccagctcg
     7021 gcaccttcac gcatatgggc gggccggggg ccggacaggc caccaagctc gtcaatcaga
     7081 cgctcgtgct taccaactac tgcgtgatcg ccgaggcgct ccgtctcgcg gagacctacg
     7141 gcatagacgc gtccaagctt ccgcaggccc tcggcggcgg atatgccggc tccaatctgc
     7201 tgccggtcat gctggagcgc atgatcccgc gcgactggac gcccaagggc tacgcccggc
     7261 aggtcctgaa agacctggag atggtcaacg aggcagcccg cagccgtcat ctggccatgc
     7321 cgatgacgag ccaggccctg acgctgttcc gcatgctggt cgcccaaggc aagagcgaga
     7381 tcgacggcgc cgccgtgctg gaagtgctgc ccccgggcga caagccgtag cggctccgat
     7441 tcgattggag cgcggactcc ctctcccaag gggcagaggg agagctgcag cccgagcgcc
     7501 aatccttact tcggtatttc gagttgcttc accgcttctg caaggaactc tgacatttta
     7561 gcacgcagct ctgcatcgga gatcgagacg cccttggcat cgaagtcacc gcggagtttc
     7621 aggaagacgt cctcgtcgcc cttctcggcc aaatcagccc gccttacaga cttcacgtag
     7681 tcttcgacct cgtcaccgct catgccgagc agaccggcgg cccattcacc aagcattttg
     7741 ttgcgccgag cctctgcctt gaactttagt tcctggtcat gagcaaactt cttctcgaaa
     7801 gctttctcgc gatcttcgaa ggttgtcatt cggtcttctc cacctctacc tcgttcttct
     7861 accaggaacc ggcctggtac aggcatgctg ccagtagcta gccaagccag cgagccatag
     7921 acggtggccg caccgagatc aataaccagt gcgtcggttt agccacgcgg caactggccc
     7981 cgccgttaag attgtttctc aacccgcctt cggctagtgt ccggcgagga attcaatcgg
     8041 gttccggctc ccttgctacg ccgcgcaggg tgtcggcgcg ctgcactcga cggatagccc
     8101 gaacccggtc gggatgcatc tgtccccaaa acaacaagcg aatgcatatg tttagaggtc
     8161 catctcacat gaacaggcgc cgtcgcatct atgagggcaa ggccaaggtg ctttacgagg
     8221 ggccggagcc cggcaccctc atccagcact tcaaggacga cgccactgca ttcaacaaca
     8281 agaagcacgc attgatcgaa ggcaagggcg tgctcaacaa ccgcatttcc gagtacatct
     8341 tcgtcaagct cggcgagatc ggcgtgccga ctcacttcat ccggcgcctc aacatgcgcg
     8401 agcagctgat ccgcgaggtg gagatcatcc cgctcgaggt ggtcgttcgc aacgtggcgg
     8461 ccggctcgct cgcccagcgc ctcggcctcg aggagggcac gcagctcccc cgctcgatca
     8521 tcgagttcta ctacaaggcg gacaatctca acgatccact cgtctccgag gagcacatca
     8581 cggccttcgg ttgggcgacg ccgcacgaga tcgacgagat catgcagctt gcgctcagga
     8641 tcaacgactt cctcgtcggc ctgttcctcg gcatcggcat ccggctcgtc gacttcaagg
     8701 tcgagttcgg gcggctgtgg gacaacgacc agatgcgaat cgtactggcc gacgagatca
     8761 gtcccgactg ctgccgcctc tgggacgtgc agacttccga caagctcgac aaggaccgct
     8821 tccgccgcga catgggcggc ctgctcgagg cctatcagga ggtcgcccgt cggcttggag
     8881 tcttcactga caaccggccg gcgaagggtg cggtgcccgt tctggttgca tcgaatcctg
     8941 ggggaaagcc gaattaggct gttcgtgagg tcgtgaagtc gtgaaatcgt gaagtcgagc
     9001 tgtcgacctt acgacttcac gacctggcga tttcacgatt tcacgcgccc tattcgccat
     9061 tcgctactcg ctattcgctt gtgaaatgaa agcacgcatc atcatcacct tgaagaacgg
     9121 cgttctcgat ccgcagggca aggccattca gaatgccgtc ggcgcgctcg ggatcgaggg
     9181 ggtccagtcc gtccggcagg gtaagtacat cgaggtcgag ctggcagcca ccagcgaggc
     9241 ggccgcccgt gagaccgtcg accgcatgtg ccgggaactt ctcgcgaaca cggtgatcga
     9301 gaactatacg tacgagatcg aggggtgatt cctctgggtc atcccggtgc ttgtcaccga
     9361 gatccaactc tcctcaagcg ccggaggtcc tgttgcggga tctgttgcag cgtgcacctt
     9421 cttaacctct cccatgggga gaggtcggcc cgaagggccg ggtgaggggt tacaacatca
     9481 gcaatcgttg agaccccctc accccgaccc tctccccact ggggagaggg agaattggtg
     9541 ctagcctgct gaggttccga gatggagctt ggggtaggtc gcagcggggt agcatgaaag
     9601 cttctgtcat catcttcccg ggaaccaatc gcgaacgcga tgtcgccgat gtgctagagg
     9661 ccgtatccgg ccgcgccgcg cagatggttt ggcacgggga cacggaggtg ccggcctcgg
     9721 acctcatcgt gctgccaggc ggattctcct acggcgacta cctgcgctgc ggcgccatgg
     9781 ccgcccattc gccgatcatg cgagacgtgg tcgccaaggc gaaggccggc acgccggtgc
     9841 ttggcatctg caacggcttc cagatccttt gcgaggccgg attgctaccc ggcgtgctga
     9901 tgcacaatgc ctcgcttagg ttcgtctgca aggaagtgtg gctcagggtc gagcagacgg
     9961 acaccctgtt cacgagccgc ttcgccaaag gcgaggtgat gcctgccatc gcggctcatg
    10021 ccgagggcaa ctacttcgcc gacaccgaca ctctggaccg gctcgaaggc gagggccgcg
    10081 tcgtctttcg ctatgccgac cgccacggca acgtcacgcc tgaagccaat ccgaacggtg
    10141 cccaacgcag catcgccggc atctgcgacg cccggcgccg cgttctcggc ctgatgccgc
    10201 atccggagaa tacctccgat cctctgctcg ggccgacggg agggcgcaag ctgttcgaga
    10261 gcatcgtcga caatttggtt gccgcctgac cgccgcaccg gcgccggcaa ggataaggtc
    10321 aatgccgtgc gccgatctgg tggccgatga tgttggcgtg ctcaccctcg tggcattcgg
    10381 aggcgcgttc gacctccagg gtcatgtggc tcacaccgaa ccgatcacgc acgagctctc
    10441 tgatccggtc cttgatcggc ttgggatcac tcgcgagcgc atcgggcacg acgatgtgcg
    10501 cctccagcga ggtgcgatgc tcgtcgatgg cccacacgtg cacatgatgg acgttctcca
    10561 cgccgtccac cttccggatc gcgtcgacga gctcgtcgac gctgatctcc ggcggtacac
    10621 cgagcatgag gagccggatg acgctcccga tctcggtgag cgccatccac aggatgtagg
    10681 ctgagatccc gatcgtcacg atcgggtcga cgatatacca gccgaacgtc aggatgagcg
    10741 cgccggcgac gatgacggcg accgagccga gcgcatcagc caggttgtgc aggaatgcgg
    10801 cgcgaacgtt ctggctcgtt ttcgccagag aaaaggtgag cagcgccgtc accgcgtcga
    10861 tcgccagcgc aacgccagca acccagacca cgatccagcc ctcgacctct gcgggtgcga
    10921 agaaccgtgc aacagcctcg taggccagat agacgccgat gacgatcagc gtcgtgtagt
    10981 tgatgagcgc cgcgatgctc tctgcccgga cataaccgaa ggtcatgagc tcgtcgctcg
    11041 ggcggcgggc gatcctccgc gcaccgtacg cgatgccgag cgaaaccgcg tcggacaggt
    11101 tgtgcacgcc atcggcaacc agcgcgaggc tgcctgcgag aatgccggcg accacctgca
    11161 cgaaggtcag ggcgacattg acgagcaccg cccaccacag gcgccgatcg ccggcctccg
    11221 cgccgatgtg gacatgaccg tgcgagtgcg ggtgcgaatg ccgtgccatc agcctgtctg
    11281 ccgccagcct tgaaaaactg caacaggcgc tggacagcgg gagttccagc gcctgcactg
    11341 cccagcaggc ccgctaccga gcatcaactc cgctcgaagc gcttgaactt gattcggtgc
    11401 ggctcgaccg cagcctcgcc cagccttcgc ttcttgtctt cctcgtaggc ttcgaagttg
    11461 ccctcgaacc actccacgtg gctgtcaccc tcgaacgcca ggatgtgcgt ggcgatgcgg
    11521 tccaagaaga agcggtcgtg gctgatcacc accgcgcagc ccgggaagtc ctccagcgcg
    11581 gcctcgagcg cagacagcgt ctccgtgtcg aggtcgttcg tcggctcgtc gagcagcaga
    11641 agattgctgc cttccttgag catcttcgcg agatgcacgc ggttgcgctc accacctgag
    11701 agcatgccga ccttcttctg ctggtcgggg ccgcggaaat tgaaccagcc gacataggcg
    11761 cgggacggca cctcgcgctt gccgagctgg atcatgtcgt tgccgccgga gatctcctcc
    11821 cagaccgtct tcttgggatc aaggtcctcg cgggactggt cgacgtagcc gagcttgacc
    11881 gtatcgccga gccggatggt tccggcatcc ggctgctcct gaccggtgat catgcggaac
    11941 agcgtggtct tgccggcacc gttggggccg atcacgccga cgatgccgcc cggcggcagc
    12001 ttgaacgaca gattgtcgat gagaagccgg tcgccgaagc ctttgacgag cccttcgacc
    12061 tcgagcacga cgctgcccag ccgcggtccc ggcgggatct ggatctgcgc gtggccgatc
    12121 tgctcgcgac ccgcctcctc ggcaagccgc tcataggcct ggatacgcgc cttggatttg
    12181 gcctgtcgcg ccttcgggct ggagcggatc cattccagct cccgcttgag cgcgcgttgt
    12241 ttggcctcct cctgcccctt ctcgacctcg aggcgctttg ccttctgctc gagccagctc
    12301 gaataattgc ccttgtaggg cacgccctgg ccgcgatcga gctccagcgt ccattcggtg
    12361 atgttgtcga ggaagtagcg gtcgtgggtc accagcatga tgcagccggg atattccctg
    12421 agatagcgct cgagccacgc cacggtctcg gcgtcgagat ggttcgtcgg ctcgtcgagc
    12481 agcaggatgt cgggcttcga caaaagcaac cggcacagcg cgacacggcg cttctcgccg
    12541 ccggagaggt tggtcacgtc ggcatcgccc ggcggacagc gcagcgcatc gagcgcctgc
    12601 tcgacctgct ggtcgagatc ccacaggttc tgcgcgtcga tctcgtcctg caggcgcgcc
    12661 atctcgtccg ctgtctcgtc cgaataattg gccgcgagct cgttgtagcg gtcgagcagc
    12721 gccttcttct cggcgacgcc ctccatggcg ttgccgagca cgtccttgtc ggggtcgagc
    12781 tccggctcct gcggcaggta accgacgcgc acgccgtcgg cagcccaggc ctcgccctgg
    12841 tagtcctcga tctcgccggc catgatcttg agcagggtcg acttgcccgc gccgttgagg
    12901 cccacgacgc cgatcttggc gccgggcagg aacgacagcg agatatcctt cagaacctgc
    12961 ttgccccccg gataggtctt ggagagccgg tgcatgtgat agacgtactg cggcgtggcc
    13021 atttgcttct ttggcgcctt ctcgctctga tctgcaacgg gagattggcc gtggttctag
    13081 acgaaaccag gcgccgtctc aactgacggc gccgctgtcg ctggcatcat gcggccagcg
    13141 ccggcaatct gaccttcgat atggccacta aagagcaaga acccaaccac ctggctccag
    13201 cggcaagcgt ggcatttcgc tgcgataagt gcctattatg cgactagagg tgctgacctt
    13261 cgcggtcggc gagctggagt gcggacccat gaccctggaa ctcgtctact tcgccgaccc
    13321 gatgtgctcg tggtgctacg gcttcgcgcc ggttgttgcc gccatcgagc agcattttcg
    13381 cgggcgtctg ccgctgcgtc tcgtcatggg tggcctgcgc gcggggcaga cacagccgat
    13441 gcgcgaacag gacaaggagc atgtccgcgg cgagtgggcc cgtgtcagtg aagcgaccgg
    13501 acagccgttc gatttcgcgt tcttcgatcg cgatgcgttc gtctatgaca cggagccggc
    13561 ctgccgcgcg gtcgtggcca tgcgcacgct cgacccgagc cgggcgagag ccttcatgtc
    13621 gcgaatccag ggcgccttct atgccgaaaa ccgcgacgtg acctcgacgg aggttctggc
    13681 cgatctcgct gccgaggaag gacaaaaccg ggacgcgttc ctggcgacct ttctgtcgcc
    13741 ggaggtgcgc aacgaaacct tccgcgactt cctgatcgcg caggagctgg gaatccatgg
    13801 cttcccgacg ttgatcgtcg gcggcggcag gcaatacacg ctcgtcacca acggctaccg
    13861 cccgctcgat ggcattcccg aggcgctcga tcagtggttg gcgaggcagc ctgaggcttg
    13921 aaccaactcc ttagcccggg ccttgtgtcc gggattcaaa cagtttgcga gcgcgaataa
    13981 gcggccatat ggatccctgt gagaagcacc gggatcactt tttttgacga gaattggccg
    14041 cgcttcaaat cgaaatcccg gtaaaaaaat atcgccccag accgaccgtg aacaccaggc
    14101 acccataaag actgtgctcc agccagacgg cccacagcga tcgggtcacg tcgtagcgat
    14161 aggcaaacag cagaccgatg aagaagctgc cggcgacggc aatcagattg ttgaaaatga
    14221 tatgggcaaa gccgaacaac gccgcattgg ccacgatcgc cgcccagcgc gcgttgccga
    14281 acagcggccc ataacggtgg aagaaaaatg ttcggtagac gaactcctgc gccaggaccg
    14341 acatcagcgg atacagcacc atgatcgtca gccaaaggcg gggcagacgg tatgggaaag
    14401 agaaaaagcc ggatgggtgc aaaatatgcg ccgcatacgc gatagcgctg cccagaacga
    14461 gaaatgtacc gatgatggcc agcaaatcac gcagcgagaa gccgcgggcc aattcgcgcc
    14521 gaagcaaaaa agtgggatcc cagagtaggt aaatgaaaaa gccgagaaga atcggctgaa
    14581 gcaccaagaa tagcggaagc cggaacgtct tcagaacgag actgacgaga atcggcgccg
    14641 cgacgaagaa cagagcaatt tcgaaagcga gtcgtacgcg ccgggccagg cccggtagaa
    14701 caatagccat atcgcgcggc agggtgcccg ccgcgctcag gccaagcatg gccttcatgc
    14761 acttgctact ccgccgcgat cagcttcttg aaggttgcgg agatggtatg ccgcattttg
    14821 tgaaaaccct tcgagctttc gctggtcatc cagttcatct gcaaggaccg gcgcggcccc
    14881 tcgaacggat ggtggccgtg ccaggagttg tccgaacgtt tgaagactag cagagttccc
    14941 tggtctggag gaacctcggc aacgaagtca tcgacatcgt taccgttgcg cagaatgcgc
    15001 agccgaccgc tcgcatgggg ccaagtctcg ttgagataga gcagtacggt gatgatcttg
    15061 tccggggaat cggtgtggat ccgaccgtcc ttgctccgcg tgtagcctct cagggaatac
    15121 atcttcggcc ggttttcaag gtcgacgccg aatttctcct cgatgatcgc ctggaactcg
    15181 gggccgtcga gctcctcgat gacctccttg atcgtcattc ccggctccag gctttcgatg
    15241 ggaaacgagc cgccgcgctc tatccgcggg tacgtatcgt tgatccggcg taggctctcc
    15301 atcgagagga aaccgggcac gatcacgtat tcatatggat cgttctcgac cttcgctgcg
    15361 cggagacgtt ccagattgag atgcaccaag gcatcatccc ctttctctcc ccgttatgca
    15421 ccacaaggtc agcggccgac cgatccccct ttatcagtca gctccgctga tcgaagcaga
    15481 tatcgccgct ccacctcccg ggcgatggtg gccgccgagt cgatgggagc gtaggtccaa
    15541 tcttcgagcc gctggacttc gacaggcaag gggcgtgtgg cgcctatccc ctgcaatctt
    15601 tggtggaacc gtcggaactt cgcacttccc cgggatggct cgaacacgta gaccggccga
    15661 ccggtggcgc acgcctcgcc ggtcatgttg acggagtcgg ccgtcacgac caagtagtcg
    15721 gccgccgcga gaaaataggg atacgggttc ggacccgtcc cgtcccagaa gatccgcggg
    15781 ctgttccgtg ttgcctcgtc cgcgatgcga atcagccggt tgtgcgtgcg ccgggaaggc
    15841 gtgatcatga agctcgcgcc cagccggccg agcgagacga gcgctgccgc gaaccgatcg
    15901 tcatcggcgt ccgtgaactt gtagctggcg ttcttgcccc cgagcacgac ggtgatgcgc
    15961 ggatgcggaa gactcgcgat ttcgggcggc agctcgcccc gaagcttcgc gaggcgttcg
    16021 ggcgtatggc tgtgcggcga ggtgggcgtg accaggacgt tcggcccgcg caaccggtcg
    16081 tgctccggca cccagatgag atccgccacg ccggggccgg tcctggggtc ctgcagaatg
    16141 atcgtgaaag ttgccccacg ggaataacgg cggatcgcgc ggatatacgg gatgctggcc
    16201 cgtccgctgc caatggcgat ggcaggccaa ggcggcgaaa gcaaagcgcc gggccgaccc
    16261 gggcggtcca ccggctggac aggtccccac ggtgggatca gccgccagaa cccgcgcgtt
    16321 gtcacgcgct tcatctcgta gtcgagcccc agcgcgtcgg cgacgccctt gcactggacg
    16381 accattccgg ccttgccatc ggtgatgatc caggctcgcg cgccggaaag cgcgcccacg
    16441 gaaggggcat taccaacgga tagatcttgc tgctgcatgc tgaggggttc tctcgaatcg
    16501 ggagggcgct gtcgatgctg cctgccgccg catacgaccc cgcgccgccc ggcgcaatgg
    16561 acatgcgctt cggcgcccag gcgtgttgcc attgacctgc aacggcccta tccacaatgg
    16621 gccccgtgat gaccgccatt atcttacgag ccttgatcaa tgcgagcccg cctggacgtc
    16681 atcgactggc gcattctgaa ggagcttcag gccgacggcc gcatcaccaa tgtggccctt
    16741 gcccagcgcg tcggcatcag cgctccaccc tgtctgcgcc gtgtgcgcgc gctggaggag
    16801 gccggcttca tcaccggcta ccacgcccac gtcgacgaga agatgctcgg gttcgagctg
    16861 accgcctttg cgatggtcgg ccttcacagc caggccgagc cagacctgcg cgaattcgaa
    16921 aaccgcgtgc tcggctggcc gctggtgcgg gaagcctaca tgatgtcggg cgagaccgac
    16981 tacgtgctga agtgcgtcgc ccctgatctc accacgttcc agaatttcgt catccgcgag
    17041 ctgaccgccg cccccaacgt cgccagcgtc aagacggccc tcgccatccg ccgcgccaag
    17101 ctcgagcccg gcgtgccgat cggctacgcg caatcgtgag ccgcgctgcg tcatccagat
    17161 gtggtcgtcc ggttgagaac tggttggcgt cgataaacac agtggcggac gatttcgtgg
    17221 cttcagcatg acacgctacg cgctctgcaa gctgcggatg atggtgcggc atatgcggca
    17281 accgaagtcc aggcatgcgg gcgaattcgt tttgttgggg cgcgagctcg tcggagcgcg
    17341 ttaaaggggg caagcaacgc tgaggagatc agccatggcc gaagccccga gcccggacat
    17401 ccggaagcca aacctggtcg gcatcaatca cgtcgccctg gaggtgggca gcatcgacga
    17461 ggcgcttgcg ttctacggca gcatcttttc cttcaagctg cgtggccggg gcaaaagcag
    17521 cgctttcatc gacatgggtg atcagttcat tgccttgatg gagagcgaca cgccggccgg
    17581 tgacaaggag cgccacttcg ggctggtggt cgatgaccgc tcggccgtgc gccagctcgc
    17641 cgaggccgct ggcgccaaag tcctgcccgg acactttctc gatttcctcg acccctgggg
    17701 caaccgggtg caggtggtcg agtacagtga cattcagttc accaaggcgc ccgatgtcct
    17761 caaggcgatg ggtctcgatc tgcgcaagag cgacaaggcg ctgagggagc tggccgacaa
    17821 gggcatcatt ttctgaccct ccacgaacac cgaagcccat gccaaagcgc agtgcaggcc
    17881 tcctcctcta ccggctgaca ggcggcgagg ttgaggtgtt cctcgtgcat cctggcggcc
    17941 cctactgggc gaaaaagaac gagggcgcct ggtcgattcc gaagggcgag atcgatgagg
    18001 gcgaggaccc gctgtccgcg gcgcggcgcg aattcaagga ggagacgggc gtctggcccg
    18061 aaggcgaagc gatcccgctc ggcaccgttc gccagcccgg cggcaagctt gtcacggcat
    18121 tcgccgtcga aggcgacctc gacgcggaca cgatccgcag caacacattc accatggaat
    18181 ggccgccacg ctcaggccgg atgagggcct tccccgaggt cgaccgcgcc ggctggttcg
    18241 ggaaggaaga agccgagcgc aagatcttga aggggcaaag agcgatcctg gacgcgctgt
    18301 ggaagatggt gacttgagga gcacgcaagc ggtaggcgga ggtgcgatcc ggtggaggtg
    18361 tcgcaagcgg gcaacggcgg ccgtgatggc tcatttttga tttgcgaggc agctggagtt
    18421 gctggacgtt ggatactcac tacactgtgg agcatcccct atgtcccagc ttactggaat
    18481 tgccatcgca ctgacattgc tgctcgccgg agcgagtccc agcgtcgctc aatcagagtc
    18541 ggaacgggag gcagtccgcg ctgcaaatca agcctattac gacgccctct cggcacgcga
    18601 tatccaagcc atggagcagg tctggtcacg ggaagctgat gacgtgcttg tcgctccgcc
    18661 aattcggccc accgcgcatg tcggttggga caagatcaag aagcaatacg aaacattctg
    18721 ggcgacgctg gaggagctga ccgtgtcaat ggaacagccc acgatccaca tcgagggaaa
    18781 cgtcgcttgg actttcggca cggagcatgc caagcgccga accaaggatg gtcaggccag
    18841 cggcggcccg aacttcggga ccaacatatt cgtcaaacgt gacgggcggt ggctgctggt
    18901 cttccaccag gcggcgctga tgccacaacc acctgctgag aaagcaaaat gatccctcac
    18961 tcgggatgac cgagagcctt cggggtattc tcggttgatc atttcagcgt attgtgcgtt
    19021 gaaatcctga atgattgacc tcgccgtcct ccatcccggc ctcacaggca cctctaccct
    19081 gcgcgtcgcg cctgagcata cggcgccgca cgtcggctca ggacgtgtgg cggtgctggc
    19141 gacgcccgtg atgatcaacg tgatcgaggc cgcggcactc aatgccgtcg agcatctgct
    19201 gcccgaggga catcaaagtc tcgggattca cctcgacgtg cgccatttcg cggcgacgcc
    19261 ggtcgggttg ctggtgacgg caaccgcgga ggtgattgcc gtcgagggac ggacgatcac
    19321 cttccgggtc gaagcgcggg atgagtgcga ggcgatcggc gaaggcacgc atcagcgcgt
    19381 catcgtgaac gtggcgcgct tcgatgagcg ggtgcagcga aaactgaaga gcttcgggta
    19441 agcctcggat tttgggcttt cgtcccgacc gagcctcccc tccccctgcg agggtgggga
    19501 gtgagacggc ggacccgggt cacgcccctt cctgctgctt cttgtactcg gcatccttgg
    19561 cttgcgcccg ctgtgcagcg gggcgtgcat tcagccggtc gacgtaagcg acgaactcgg
    19621 ggcgctcagg aatgagcttg atcatcatgg cccagttcag ccccgagccg atgaccacgt
    19681 ccgccgcggt gaactggctg cccagcaggt agggatcggc ctcagcggtg gccttcgcga
    19741 gcacgtccat caccaggtcg aaatttccga agcccagtgt cgcgcgcggg ggctcctcct
    19801 tgcgcggaaa cgcccggtcc gtgatcgccg gctcgaggca actcggaccg aagaacagcc
    19861 atttgagata agggccgcgc cgcgggtcgc cggtcgggat gttgagcttg gcctgcggga
    19921 actcgtcggc gagataggtg cagatggcgg ccgactcggt gatcaccgcg tcgccgtgcc
    19981 ggagggcggg caccttgccc atgggattga tggccagaaa ctcgggctga agattctcgc
    20041 ccttcttgaa gctgataagt tgcacgtcgt acggctggcc gagctcctcg agcatccaaa
    20101 gcacaacgga cgagcgcgat ggcgcagcgt ggtagagggt gagctttgac atccttcgac
    20161 ctcctcgatc ggtagggccc gaggggctaa ccgccgagtg aggcgattcg atgtatcaat
    20221 tctcgtttct ctcgggcgat ggcggcccga tcagcggcag acgaggaccg gaatgttgct
    20281 gtgggtcagc accttttggg tctcgctgcc gagcaggaaa gccgaaaggc ctcggcggcc
    20341 gtgggaggcc atcacgatca gatcacaccc cttgtcgttg gcagtcttga tgatgccttc
    20401 atagggctga gggctttcga gctgcaccag ctcgcaaggc acacccctgg cctccgccgc
    20461 tttggcgacg acattcaagg ccgccttggc ctgcgcatcg atcgattgct ggtatttttc
    20521 cggatcctcc ggataaacga cgccctcgac ggtcaacagc tggagcggcg gcgtgaccgt
    20581 gacggccgtc aaccgcgcac cgaccagttt tgcgagctcg atggcatggc cgaaggcctt
    20641 ctcggagaat tccgatccgt ccgtcgtcac gaggatgttc ttgtacatcg cgatctccct
    20701 gcgcgccagg atcgcctccg ccgaggtgcc ggcgccttga tgcgcatcaa tctctcacaa
    20761 cgcgcagttg tttcacaggg atgatgccga cgctgatcgg ttcatcggcg tcaattcgct
    20821 ttcgacgatg ggcagtagag gtcgtacagg gtcgcgagca ggcgctcgat ccgcgggtct
    20881 gcgatgcgat accagagcgt ctgcccctcc cggcggtagg ccagaaggtt ctgctcacgc
    20941 atgatggcca aatgctgaga gagagccgac tgcgacagcc cgaccgcctc gccgaggctc
    21001 gtgacgttcg cctcgcccca ttccaagagt ttgcagagga tcataagccg gcgctcgttg
    21061 gaaagcgcgc gcaagacgcg cgcgacctcg gcggcgcccg cctcgaattc ggcgatgtcg
    21121 aaaagggccg ggctggtcat cgcacctttc cattagctgg cgctaaacta ggtgcggctt
    21181 atatcttgtc aatgcgcgag atgtgctccg ccgtcacgtc gaacggctct cgggcattgg
    21241 ccgaggcgca cgtaagtggg ccgccgcgcg ctgattggcc cataaagatg tccactgccc
    21301 taaaagcgat cccgccggag gttcgctaga tccgttccgg ccagtcagag ccacgcggct
    21361 cgtcgcggcc gtgcacctgg gagggcggtg ccgtggcctc gacggcggtg aagtcctcca
    21421 ggatcttgta cgaatgcttt gcccccgctg gcacgagcca ggaatctcct ggctccagct
    21481 tcacggtctg gccttcgagc tcgagctccg cacggccgtc gatgacatag cctaccgtct
    21541 cgtactcgcg cacgctttcg tccttgcggc tgccgggcgg ctcgtgctcc cagagccgca
    21601 tcgagacccg cttgcccgaa acgagatact tctggcccat gtcacccttg ggcgagtcgg
    21661 gcgcgtgaac ctttttcacc gtcgtgtccg tcatcgtaga gttccggctg aggcctcgcg
    21721 cggccatcgt tgctggcggc gcgagcgttc gtaaccggaa acctttgaaa cagaggtgcg
    21781 ttccagacca cgatgcagcc gagcgcccac gcgttcactt atcagcctta ccgcgcagga
    21841 actcgatgat gcccgagaaa tcgactcctg cgttcccagc cgcaacgaag agctgataga
    21901 gctgcgcggc ctcggcgccc aatggtgtcg ccacactggc agcacgggcc gcctcctgcg
    21961 ccaggctgag atccttcagc atcagcgcgg ccgcgaagcc gggcttatag ccgttgttgg
    22021 ccggcgaggt cggcaccggg cccggagcgg ggcagtaggt cgtcagcgac cagcactggc
    22081 ccgaggaggt cgaggcgaca tcgaacagcg cctgtttcga tagtcccagc ttctcggcga
    22141 gattgaacgc ctcgcagacg ccgatcatgc tgatgccgag aatcatattg ttgcagatct
    22201 tggccgcctg accggcgccc gcatctccgc aatgcacgac tttgcggctc accttctcga
    22261 gcacgggccg ggccttctcg aaggcctctg tgctgccgcc ggccatcagc gtgagtgtgg
    22321 cggcctccgc tccgccgacg ccgcctgaga ccgggcagtc gagcgacagc ttgcccgcgt
    22381 tacccgccag ctcgtgtgcc cgtcgggccg aagcgacatc gatcgtcgag cagtcgacga
    22441 gcaccgcccc gcttgcagcg gccgaaagca ccccggtctg ctcgtagaca gcgatggtgt
    22501 ccttgccggc gggcaacatg gtgatgacga cctccgcgcc ctcggccgcg gcatcggcgc
    22561 tttgcgccgg ggtgacgccg cgctcggcgg ccctttcgag gttggcaggc acgaggtcat
    22621 atccgaccac ggtgtgccct gccttcacga ggttgccagc catcggcgtg cccatgttgc
    22681 cgaggccgat gaatgcgatc ttggtcatgt cctcctcccg gacttcactt ccacaagcgt
    22741 gatgatcagc gcccggtcca ttgcggcggc cgtttggcga ggaaggcctc cagcccttcg
    22801 cggaagtccg cgctcgtata gaccttcgcg accagatcgg tatcgtccac cttggggccc
    22861 tgctggcgca agcgaaggag aatttccttg gtcaccctca gcgtcagcgg agcgtagccc
    22921 gcgatctggc gcgcgagctc ctcggcccgg gtcatcaggg ccgcatggct ctcgacgact
    22981 tcggacacga gacccgccgt cagcgcctcc tccgcaccca ccaggcgggc ggtgaagatc
    23041 atgtccttca cccgcggctc acccaggagc cgcaccagac gctcgagagt tgcagcagaa
    23101 aggcagttgc cgagggtgcg ggcgatcggg aagccgaact tcaggtcccg cgtggcgatc
    23161 cgcatgtcgc agcaggcggc gatgccggcg ccgccgcccg tacaggcccc cgcgatggcc
    23221 gcaatggtcg gcaccgggca ggactcgatg gccgtcatga tctcatcggc atgcttttcg
    23281 tattggatgc cctgctcagg cgtgccgaaa tcacggaaga gtgaaatgtc tgtccccgac
    23341 gcgaacgccc ggtcaccggc accggtgatg atgatcgcct tcagcgagcc gtccgtcggt
    23401 gcattggcgc aaatctcggc gatccggtcg tacatgccga atgtcaacgc gttgcgtgcg
    23461 tgcggcctgt tgagggtgag aaaaccgatc cggtcccgca cctcatagat gatctcctgc
    23521 gcgctcgcgg ccgtttgggc acgatccatc tcaacctctt ctcgcttgtc gttgtcggga
    23581 cagccccgcg tgtggctcgc gcacaggaaa ccgcctcgcc tctcgaatgg caatgacttt
    23641 caaaccgccg cgacgcaggc gggctgacgc aggcagaagt cgtcccgagc ggcatcggcg
    23701 ttgatgatgg acgccagcgc cttcggcctg tacctttgcc atttcccggt gcttcaaggc
    23761 cgggcgtaca gaacaagcgc catgtcgcgt tagggaggaa tgggaacgat gcggtcgaca
    23821 ccgcacgatc cgccgtcggc agtgtccagg cggctgccga tccgttcacc gcaagacttc
    23881 gcgggcggca tgctgctcct cgcgattgcc gtgctcggct ttctcggctc gtggcatctc
    23941 gacttcggtc aattcggcgg tgtcggctcc ggcatggtgc cacaggctgt ttcagccatg
    24001 atcgccggct tcggcctgct gctcattgtg cagggctttg tttcctcgga tggagcgcag
    24061 atgacgctgt ggtccatccg cggcctgttc ttcgtgctgg gcgccaccct gctcttcgcc
    24121 tggaccatcc gcccgctggg cctgatcttc gccgggccga tcgccatcgt gttcgcctcc
    24181 cttgctgatc gcacgacgcg ccccctggag atcgccatct tcaccgtggc gctcactgca
    24241 ttctgcatcg gcctgttcag cttcctcttg cagctgccga tcccggtgct cccgtcctcg
    24301 ctcccctacc cgttcaatga gatcttctag ggcttcctct tgacggaact gaccgaccat
    24361 ctcgcgctcg gattcgctac agctctgacg ctgaagaacc tggggctctg cttcctcggc
    24421 tgcatgatcg gatcgctgat cggcgtgcta ccgggcgtcg gacccatcgc gaccatcgcc
    24481 atgctgctgc cgttgacgtt cggcgtcgac ccggtgggcg cgctcatcat gctggctggg
    24541 atctactacg gcgcccagta tggcggctcg accacggcca tcctcgtcaa tatccccggc
    24601 gaggcgacct ccgtcgtgac cgtcctcgac ggccatcaga tggcaaagca gggccgcgcc
    24661 ggcatcgcgc ttggcatctg cgcgattggc tccttcatcg cgggcaccgt cgcgacgctc
    24721 atcatcgctg ccctcgggac gccgctgacg aaaatggcgc tgctgttcgg accggcggat
    24781 tacttctcac tgatggtgct cggcctcatc tttgccgtgg tgctggcgag cggatccgta
    24841 ccaaaggcca tcgccatcat cctggtcggg cttctgatgt ccacgatcgg catcgacatc
    24901 gagaccggag aggaacggct gacgtttggc ttcaacgagc ttgccgacgg aataccgttc
    24961 gccgtagcgg ccatgggcat cttcggcttt gccgagatca tccggaacct ggaggtggcg
    25021 gaaacgcggg acgtggtcag agcccccatc ggccgcctgt taccgggcct tcgtgagctg
    25081 cgccagtcgg cgctgccgat cgcgcgtggc acgctggtgg gatccattct gggcctgctg
    25141 cccggcaatg gcgccgtgtt ggggccgttc gcgtcctaca ccctcgagaa aaagctcgca
    25201 gccgagccgg agcggttcgg ccagggtgcc atcgaaggtg ttgccggacc tgaatcggcc
    25261 aacaatgccg gggcgcagac ctcgttcatt ccgcttctga cgctcggcat ccctcccaac
    25321 gcggtgatgg cgctgatggt tggtgccatg acgatccacg gcatcgtgcc cgggccgcag
    25381 gtcatccagc atcagcctgc cctgttctgg ggcatgatcg cctccatgtg gatcggcaat
    25441 ctgatgctgc tgatcatcaa cctgccgttg atcggagtct gggtgcggct gctgaaggtg
    25501 ccttaccggc tgatgttccc ggccatcgtc gtcttttgct gcatcggcat ctattcgatg
    25561 aacaattccg gacttcaggt cttcatggtc gccgttttcg gtttgttcgg atacatgctg
    25621 gtgaagttcg ggttcgagcc ggcgccgttg ctgctcggct tcgtgctcgg ccagttgatg
    25681 gaagaaaagc tgcgccaggc gctggtgatc tcgcgcggca gcttcattac gtttttcgac
    25741 aggccggtca gcgcgctcct gctggcattg gccctcgctc tggtcgtcgt cgcagtgctg
    25801 ccctccatcc gcagcaggag ggacaccgta ttccaggagt gatccggagg cgctcagcgg
    25861 acccgcagcg ccccgaatca atcaacaacc catggttgcg tggataccat tcccaatgcg
    25921 acgtttcaga agctcgcgtg gacccggcag cggccaagct tctgcttctg ttcgggatca
    25981 ctatcgtgtg ttcttctgtc gcgcggcaca cccggggcta gcgcggtcca gcattctacg
    26041 tttccattcc tcgaaccaag gatctgacac atcggttcac aatggcaatc aatcgactga
    26101 gacaccgttc acgcgaagtc gtcatcgcgc tcgccatcac cctggctgcg attgcagcgg
    26161 tgatcgcgac tctgtcgagc caggaggagg acgcccaaac agcagaaccg atggcgctcg
    26221 tcacaccccg caagtagagg cttgccccgg tgacccggac gccatcgaca gctgtcactg
    26281 cgctgtccat cccccgtcga tcgagtaatt ggctccggtg atcgaggcgg ctgcatcgct
    26341 ggccaggaac gcggcaagcg cgccgacctg gtcgacggtg acgaactccc gcgtgggctg
    26401 ccgcgcgagg atctgggccc gggcctcttc ctcggtgagc ccttgcgtac gggccagatc
    26461 agggatctgc ttttccacga gaggcgtcca cacgaaaccg ggcgagatcg cgttcgccgt
    26521 cacgccgtgt tcggccgtct ccagcgcgac cactttcgtc aacccggcca gcccgtgctt
    26581 ggctgccaca taggcgctct tgttgaccga agccctcagc gaatgcgccg agcagatgtt
    26641 gatgatgcgc ccccagcggc gcgccttcat gccgggcacg acggcgcgga tggcgtggaa
    26701 ggccgcggac aggttgatcg cgatgatggc gtcccacttt tcgatgggaa actcctcgat
    26761 cggcgagacg tgctggatgc cggcattatt gaccaggatg tcgacctgcc cgaacgtgcg
    26821 ctccgcctcc gcgaccatgt ccgcgatctg gttgtggcgc agcatgttcg ccggatggaa
    26881 ggcgacccga gtaccgtgcg ctgccacctc ccggatctga ttggcgttct cattctcgtc
    26941 gccgaggccg ttcatcatga cgttggctcc cgccttcgcg aaggcatggg cgatcgcgag
    27001 cccgatgccc gacgtcgagc cggtgacgat ggctgacttg cccttgagtt gcatgacacc
    27061 tcctcagtcc gcttggccac tcctgcgaca cgcgcaacct acactgcgat cgatcctgct
    27121 gcgagccgct gtcttactcc aggcgaaatc cgtcgagccg gtaaaatgta accctgcgcg
    27181 ccggcccagc gccggcaggc acacgtcgct ccgtcaatat ctccgcgatt gcaaagcggt
    27241 ccgacagctc gcctgcatcg gggcgcaacg tcaccagcag cacgggctca ggtgctcctt
    27301 tccggtaggc tcgggtgagt tcatagtgat cgcgcgggcg cacccccggc tcgcgccagg
    27361 cgaaaaccgg cgtgggctca tcgcgcatgt aatagagcaa ttcagccgtc accgcgcgat
    27421 catcggtcag cacggcgccg aacggtcgtc ctgcccgcct tgcctcgtca aggacagcgg
    27481 ccgtctcttc ggcaagcgtg cgccagccga gcgttcgtgc gaagggatct ccgcccccgg
    27541 ggagccggaa cgatcccgct ttggccgtcg cgatgccgat gccgacgaga atggccgcgt
    27601 gcagcgcgag agaccccttc agccaccgcc aggccccctc ccgcagcatc gtcgtcgtca
    27661 caaggactgt cgccgagaca tagctcactg cagcccagtt ggcgtgcgcg cgcgagagga
    27721 ggccctgcac ggtgaccagc aagatcaccg gaaccgcaaa cgccagcatc agctgatccg
    27781 gcttcggcag gccctccagc catgcgcgcc aggtgatcac cagaagcgat ccgaacagga
    27841 tcggcccgaa caccccgaac tgagcgccga agaactccag cgccttgttt ggatggaaca
    27901 gcacccccgt ccacttggca ttgtctgccg tgtgggcgag cgtggcgaag ccgttctcga
    27961 tgttccaggc aaggttcggc gcgatcagca ctagaccgat gagcaaggca gcgtagaggc
    28021 gcgcatcgcg cagcaaacag cgtccttgcg gcgccaccaa catgaacgcg gccgccgaga
    28081 acacgaaaaa cgccatcgca tacttcgcgt tgagcccgag gccgaaggcc agtccgagca
    28141 acagcgcggg ccaccatcgg tccgtctcga gcaggcgcac caaggcatag agcgccaccg
    28201 cccagaaaaa cagcagcggc acatccgtgg agatgatgcc ggccgatagc gaaacccccg
    28261 gcagcgtggc gaaaaccact gctgaccaga acccggcgcg cctgtcataa aggcgcgcgg
    28321 tcagcgcata gatcaccagt gccgtcgccg tatgaatgat cggggacggc agccgcacgc
    28381 agaactccga gctcccgcag gtctccgtcg ccgcgcgaat gatccaggcg atcagcggcg
    28441 gcttggagta gtagccgaag gccggctcga ggccccagga ccagtattgc gcctcgtcga
    28501 agaacaggtc ggtgccattg aaggccaggg cgacgaggcg gatcgccagc agcagtccga
    28561 gcaccgcgag aagcgccggc agccagccgg cccggacgcc cgcatcgctt ccacgcgcgg
    28621 catgcaccgt catgtcgtcc ccctgctttg ccgaccgggt ttcggcccgc cgcccattta
    28681 catggttgag cagccctcgc aaaccctgat acctttggtc gaacgcgatc gggggcacaa
    28741 ctgcttgccg cgcccgcatc acaggaacgg cactcaccta tgcgcatatc ggtggtcatt
    28801 cccgccctca acgaggccgg caacatcggc cgtctcgtgc aggaaaccta cgcgaccgtt
    28861 cctgccgaca ctctggccga ggtcattgtg gtcgacgatg caagcaccga cgccaccggc
    28921 gccgaggtga aagcgctgat ccagtccaac gacttccccg gactccgtta tctgcggcac
    28981 ggccagcgcg ccggccagag cgccgccatg cggacgggca tcctggcggc caccagtccg
    29041 gtgatcgcc
//
LOCUS       DASVDU010000238         4577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1410121, whole genome shotgun sequence.
ACCESSION   DASVDU010000238 DASVDU010000000
VERSION     DASVDU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410121"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VF226_15365"
     CDS             complement(<1..979)
                     /locus_tag="VF226_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX7075418.1"
                     /translation="MDERLRFVARLLEGEQMAPLCREFGISRVTGYKIFNRYKECGLD
                     ALNDRSRRPYRHANKLACQVERCILGIKREYPSWGAPKVREKLIRQFPMIKAPAISTV
                     HAVLDRNGLVRRRKRRRYKAEGTDLHAAQDANGLWCADYKGEFMLGNRQYCYPLTITD
                     YRSRYLLACEGMASTRSDFAFALFERVFKEFGLPAAIRTDNGAPLASANALFGLSKLS
                     AWWLRLGIALERIKPANPQQTGRHERMHLTLKQEATKPASFNFLQQQERFDDFVRIYN
                     NERPHQALGGACPGEIYTPSARVYRPPEEPHYPFHDRTMRVTRCGRICIG"
     gene            1547..3043
                     /locus_tag="VF226_15370"
     CDS             1547..3043
                     /locus_tag="VF226_15370"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX7075419.1"
                     /translation="MAGQSATNTGSTMGSGAPSPSDRNSLTIGPDGPILLHDVHFLEQ
                     MAHFNREKVPERQPHAKGSGAFGVFEATEDVSRYTKAALFQKGAKTEMLARFSTVAGE
                     QGSPDTWRDVRGFSLKFYTTEGNYDLVGNNTPIFFVRDPMKFPHFIRSQKRLPDSGLR
                     DSHMQWDFWTLNPESAHQVTYLMGPRGLPRTWRHMNGYGSHTYMWGNAAGEKFWVKYH
                     FHTDQGLEFFTNAEAAAMAGADADFHRRDLFEAIAKGDYPSWTLSVQIMPYEDAKTYR
                     INPFDLTKTWPHADYPLVRVGKMTLDRNPENFFAQIEQAAFSPGNIVPGIGLSPDKML
                     LGRAFAYNDAQRNRIGANFHQLPVNQPKVPVNTYMFDGHMAYHHSGAAPVYAPNSGGR
                     PWADETGVMPDNWEADGEMVRSAYTLRPDDDDFTQPGILVREVFSDADRDHLVETVAG
                     ALLGGVREPVLSRAFEYWRNIDPGVGGRIEAKVREGRAQKPAQGMGER"
     gene            3618..3932
                     /locus_tag="VF226_15375"
     CDS             3618..3932
                     /locus_tag="VF226_15375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7075420.1"
                     /translation="MASHDDRQAGEGMAEDVIENSELHRFELALDDGEAVAYYRSEGD
                     RVVLIHTEVPQQYAGRGIGSRLAKGVFDNLRKTGRKAVVKCSFMAAWASRNPEYNDII
                     AG"
     gene            4004..4402
                     /locus_tag="VF226_15380"
     CDS             4004..4402
                     /locus_tag="VF226_15380"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7075421.1"
                     /translation="MPESVVEQTQPQGELAIRTVAMPADTNPNGDIFGGWVLSQMDMA
                     GGMIATRRAHGRVATVAIATMSFHRPVFVGDEVSCYGEITRIGNTSITIKVESWVRRG
                     RGGDFLKVTEGLFTFVAIDEDRRPRKVPPE"
BASE COUNT          962 a         1378 c         1409 g          828 t
ORIGIN      
        1 tgccgatgca gatgcgaccg cagcgggtga cccgcatggt gcgatcgtgg aagggatagt
       61 gtggctcttc gggcgggcga tagacgcgcg ccgagggtgt atagatttcg ccgggacagg
      121 cgccgcccag ggcctggtgg ggacgctcgt tgttgtaaat ccgcacgaag tcgtcgaatc
      181 gctcctgttg ctgcaggaag ttgaatgagg ccggcttggt ggcttcctgc ttgagcgtca
      241 ggtgcatgcg ctcgtgacgg ccggtttgct gcggattggc cggcttgatg cgctcgaggg
      301 cgatgccgag tctgagccac cacgccgaga gcttggacaa gccgaacagg gcgttggcgg
      361 aggccaaagg cgcgccgttg tcggtgcgga tggcggcggg caggccgaac tccttgaaga
      421 cgcgttcgaa cagggcgaag gcgaagtcgg accgcgtgga ggccatgccc tcgcaggcca
      481 ggagatagcg ggaccgatag tcggtgatgg tgagcggata gcaatactgg cggttgccga
      541 gcatgaactc gcccttgtag tcggcacacc acagcccgtt ggcgtcctga gcggcatgca
      601 ggtcggttcc ctcggccttg tagcgccgtc gcttgcgccg cctgaccagt ccgttgcggt
      661 ccagcacggc atgaacagtg ctgatggcgg gcgccttgat catcgggaac tggcggatca
      721 gcttctcgcg caccttcggg gcgccccagg aaggatactc acgcttgatg cccagaatgc
      781 agcgctcaac ctggcaggcc agcttgttgg cgtgccgata aggccggcgg cttcgatcgt
      841 tgagggcatc gaggccgcat tccttgtagc ggttgaagat cttgtagccc gtgacacgcg
      901 agatgccgaa ctcacggcac agcggcgcca tctgttctcc ctccagaagg cgggcgacaa
      961 agcgaagacg ttcatccatt ggcttacact cgttccaagg catccggggg ctccccaaat
     1021 gcccgacgtg taaaccttgt ctccggaatg aactgtcacc cttctctcgg gaagggcaaa
     1081 aggtccacat gtcagcatca ggccattccc gaacaaaaag ggagagaact tcggctgacg
     1141 tcggccagac gtggaaaacg acagctctgt gacaggaagg gcgaagaacc cgacttgtta
     1201 taagtgcggc tctaaccgac taagccagaa tcgcgcacca tgcgaatgga cgccttggct
     1261 taggtccgct gtctctttca tttacgagag gccggcggtc acccagggcc gcaaaaatgt
     1321 caagcctctg ctgcgatggc cctcgcgctt gaagccgaaa gcgcaagcat ttcccgctct
     1381 atagacgctt tgacataagg accttgggct gacaagatga gttagaccga ggggacaggg
     1441 gcatctctga agagagatgc tttgccgtaa tttggcttca gttcagattt cgcggcaacg
     1501 ataccgaata cgctgttcga tagttgccct caccgaggag aagagcatgg caggtcaatc
     1561 ggcgacgaac actggctcca ccatgggatc gggcgctccg tcacccagcg atcgcaattc
     1621 gctgaccatc ggccccgacg gcccgatcct gctccacgac gtccacttcc tcgagcagat
     1681 ggcacacttc aaccgggaaa aggttcccga gcgtcagccc cacgccaagg gatccggggc
     1741 tttcggcgtg ttcgaggcga ctgaggacgt ctcgcgctac accaaggcgg cactgttcca
     1801 gaaaggcgcg aagaccgaga tgctggcgcg cttctcgacc gtcgccggcg agcagggcag
     1861 ccccgacact tggcgcgacg tgcgcggctt ctcgctgaag ttttacacca ccgagggcaa
     1921 ttacgacctc gtcggcaaca acacgccgat cttcttcgtg cgcgacccga tgaagttccc
     1981 gcacttcatc cgcagccaga agcggcttcc cgattcaggc ctgcgcgaca gccacatgca
     2041 gtgggacttc tggacgctga accccgaatc ggcgcaccag gtaacctacc tgatgggccc
     2101 gcgcggtctg ccgcggacct ggcggcacat gaacggttac ggctcccaca cgtacatgtg
     2161 gggcaacgcc gctggcgaga agttctgggt caagtaccac ttccacaccg accagggtct
     2221 ggagttcttc accaatgctg aagcggcggc gatggccggc gccgacgcgg atttccaccg
     2281 ccgcgatctg ttcgaagcga ttgcgaaagg cgactatccg agctggaccc tctcggtgca
     2341 gatcatgccc tacgaggacg cgaagaccta tcggatcaac ccgttcgacc tgaccaagac
     2401 ttggccgcat gcggactatc cgctggtccg ggttggcaag atgacgctcg atcgcaatcc
     2461 cgagaatttc ttcgcccaga tcgagcaggc ggcattctct cctggcaaca tcgtcccagg
     2521 catcggcctc tcgcccgaca agatgctcct tggccgcgcc ttcgcctaca acgacgcgca
     2581 aaggaaccgg atcggcgcca atttccatca gcttccggtc aaccagccca aggtgccggt
     2641 gaacacctac atgttcgacg ggcacatggc ctatcatcac agcggggcgg cgccggtcta
     2701 tgccccgaac agcggcggac gaccctgggc tgatgagacc ggcgtgatgc ccgataactg
     2761 ggaagccgac ggcgagatgg tgcggagcgc ctacacgctg cggcctgacg acgacgattt
     2821 cactcaaccc ggcatcctcg tgcgcgaggt gttcagcgac gcggatcgcg accacctggt
     2881 cgagaccgtc gcaggcgccc tgctaggggg cgtccgtgag cccgtgctga gccgcgcatt
     2941 tgaatattgg aggaatatcg atcccggcgt cggcgggcgg atcgaagcca aggtccgcga
     3001 aggtcgggcg cagaagcccg ctcagggcat gggcgaacgt tgaaatactg acccttgtgc
     3061 ggggcagaca tctgccctgc acctcaagcg cattgatcgt gcaagcgcca gcagggcgct
     3121 tagggccaac cggcagtcca cgccaccagc gatagcccaa tgacaaggaa tagtgaagcg
     3181 gcgatgcgaa cgacggagag cggatacgct tgatcgactc gttgccgagc agcactgttg
     3241 gagcgctggc gatcatcatc ctagagttgt tccggcggtg gcgactaaga ccgagcggta
     3301 gtctgccagt gacagcgcaa tttgtctcgc cagagcgact cgccactccc tgcatgccat
     3361 gatctctcgg ccaagtatat cgacacacaa gtccgctttg ctgcggctcg accagactcg
     3421 atcagagatg atcatgacgg aaatcggaca taatgctgcc gttatcgcag gatttccgat
     3481 cgctacacga cgatcagcca gtcctcattt tcatcatcgg ctgcgacact tcctcgcgcc
     3541 ctgtcgttct gccgcggacc ctcgagaaga tgccgaccaa aaacacatcg taactctgag
     3601 atacaaacca tatcgccatg gcgagtcacg atgatcggca agcgggggag ggcatggcgg
     3661 aggacgtcat agagaattcc gagcttcatc gtttcgagct tgcgctcgac gacggcgagg
     3721 cagtcgccta ctaccggtcc gagggcgatc gcgtggtgct gatccacacg gaggtgcctc
     3781 aacagtacgc aggtcgcggc atcggttcgc gactggccaa aggcgtcttc gacaaccttc
     3841 ggaaaacggg gcgcaaggcg gtcgtgaaat gttcgttcat ggcggcctgg gccagccgga
     3901 atccggaata caacgacatt attgccggtt gactgggaac cggccaaagg cattgatact
     3961 gccgctgtcc gcgaccgagc atcattcgcc tatggaggaa tctttgcccg aatccgttgt
     4021 cgaacaaacc cagccacaag gcgaactcgc catccgcacg gtggccatgc cggccgacac
     4081 caacccgaac ggcgacatct tcggcggctg ggtgttgtcg cagatggaca tggccggggg
     4141 catgatcgcg acgcggcgcg cccatggccg tgtcgcgacg gtcgccatcg cgaccatgag
     4201 cttccaccgc ccggtattcg tcggcgacga ggtgagctgc tacggagaga tcaccaggat
     4261 cggcaacacc tcgatcacca tcaaggtcga gagctgggtg cggcgcggcc gcggcggcga
     4321 ctttctcaaa gtcaccgaag gtctgttcac cttcgtcgcc atcgacgagg atcgccggcc
     4381 gcgcaaggtg ccacctgagt agtagaagac ttattcttca tgatttagag tacgacacca
     4441 atagcatacc cggggggcaa gaaaaatgac gcagaatctt ccagctggcg gtggtgaaac
     4501 agtagaaaca gtaactctcg gagactctgg aggaattagt cttccaaaac ttcaacagat
     4561 ctatcacgaa ctaattc
//
LOCUS       DASVDU010000239        21052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1526423, whole genome shotgun sequence.
ACCESSION   DASVDU010000239 DASVDU010000000
VERSION     DASVDU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21052
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526423"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1864
                     /locus_tag="VF226_15385"
     CDS             47..1864
                     /locus_tag="VF226_15385"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX7075422.1"
                     /translation="MMFQSFEAPTGLENAVDRVKQLRALMAQLGVDALLVPRADEHQG
                     EYVPASAERLKWVTGFSGSAGLAVIARQSAALFVDGRYTVQARAQVDTKTFEILQVPQ
                     ARPSDWLSQKLKSGAVVGFDPRLHTVSEVERLTDALAERGIKLRPLSRNPVDRLWGAN
                     RPPPPAGKVRPHPLQYAGRSAEEKIAALQKSLKEDQQDAVVLTLPDSIAWLFNIRGSD
                     VAHNPVPLAFAIVPASGKPELFIAREKLDADARAHLSFAKVSSPDKLAERLARLKAAN
                     KRVRLDPDTASWWFARQLGGARKIVRGQDPCILPKARKNETEIKGARAAHLRDGAAFA
                     RFLAWLDREAPSGKLDEISVARKLEEFRAETQALKDISFDTISGAGPNGAIVHYRVTE
                     ATNRQLKPGELYLVDSGGQYLDGTTDITRTIAIGQPTAEMCERYTLVLKGHIAIATAR
                     FPKGTRGIEIDAFARRALWEKGLDFDHGTGHGVGSYLSVHEGPQSISRRGMAPLEPGM
                     IVSNEPGYYKEGAYGIRLENLVLVTGPEIPPGGDREMLSFETLTLAPFDRRLIRPDLL
                     TSAELDWLNSYHARVGKIIGAELGPDDRAWLAAATAPIG"
     gene            complement(1963..3516)
                     /gene="hutH"
                     /locus_tag="VF226_15390"
     CDS             complement(1963..3516)
                     /gene="hutH"
                     /locus_tag="VF226_15390"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX7075423.1"
                     /translation="MTKALELVPGDVPLAQWRAIYEGAHVSLLTPASRHRLQAAADAV
                     AAIVRKGEPVYGINTGFGKLASVRIAPADLEALQRNIVLSHAAGVGDPAPAPVVRLMM
                     ALKLASLAQGASGVRLATVELLEAMLARGLTPVVPQQGSVGASGDLAPLAHLAAAMIG
                     VGQIAIDGKNVGAAEALASVGLAPLTLGAKEGLALLNGTQFSTAYALAALFEAENLFR
                     SALVTGALSTDAARGSDAPFDPRIHALRRHRGQIETADALRALLAGSEIRASHLTGDE
                     RVQDPYCLRCQPQVMGAALDVLRQAASTLATEANGVSDNPLIFPEDGVALSGGNFHAE
                     PVAFAADMIALAICEIGSLAERRIAMLVDPVLSGLPAFLTPKPGLNSGFMIPQVTAAA
                     LVSENKQRASPASIDSIPTSANQEDHVSMAAHGARRLLPMIENAVSVIGIELLAAAQG
                     CDFHAPLKSSRILEQVRARVRAEVPPLNDDRHLHPDLVAAGRLVRSGDIAAIADDILP
                     TISGVRTGL"
     gene            3997..4623
                     /locus_tag="VF226_15395"
     CDS             3997..4623
                     /locus_tag="VF226_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX7075424.1"
                     /translation="MGGPKPQRLIVGISGASGAAYGVAALRILRELGFETHLVMTRAA
                     VITAAHELSLKVHDIQELASVVHRVDDIAACIASGSFQTLGMLVAPCSIRSLSEIAYG
                     TTSNLLTRAADVVLKERRRLVLMVRETPLHLGHLKAMTQATEAGAVIMPPVPALYAKP
                     GSIDDMVRHSVARALDLFGIDAGIVDRWGEQPLLATANTRGSAKSDAP"
     gene            complement(4642..5334)
                     /locus_tag="VF226_15400"
     CDS             complement(4642..5334)
                     /locus_tag="VF226_15400"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7075425.1"
                     /translation="MGRLDLADREHAEASGGETTVADIVRSLEFDILFGALRPRERLV
                     EDALMARFSAKRHAVRQALVKLEHMGIVVRAPNRGASVRDFTSEEVEDLAELRETLHS
                     RAVQRMPLPATPELISALTAIQRRHDKAVAARDPRRIDEANEVFHRTIFEACGNKFLI
                     DAIGHYAYLSRAMRLYPLVDPKLLETLRLEHWAMIDALKRGDRRELARLVKGHIQHSK
                     KLYLRVRGAILD"
     gene            5536..6810
                     /locus_tag="VF226_15405"
     CDS             5536..6810
                     /locus_tag="VF226_15405"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7075426.1"
                     /translation="MRSKLLSVLAGAALAWNALAAQAAAQTKEITFWSHWAAEMPKRT
                     FVEKVIADFEAKNPDIKIKATWYEKTALYAALKTALRAGQAPDVFYAEPDQVEYMDSG
                     FLLDLSELNWAAVEPWAKEAWMYKGKPYGLPLEAWTVELYYNKKMLDELGVKLPDNLQ
                     LSPDAFLDMVKKAKAKGITPMSLGAGDRPFTGAHLVHEALLKKLGVEDYSKLLAGKLA
                     WTDPRVVETLTWIRSLTDAGLLPATFTTMKLAEAHIYFHTNPGALTFLNGSWYTSRAF
                     NPVDKGGQPEDFQLGIMQFPSVPGAVCNECRTIAVGGSYVANAETEHKAEVIKFLNSF
                     LSPEVGNEWLENVKVQTGIKSDPSKMNDKQASEYFAMIAKTNAGAKYHFGLPIQVMTG
                     KPKEVFTQVFNNAFPAGNISVEDAVKQMDAAN"
     gene            6896..7810
                     /locus_tag="VF226_15410"
     CDS             6896..7810
                     /locus_tag="VF226_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7075427.1"
                     /translation="MPAFRAKELKGDVRQPWGTIVAFLLPALTFYVALTAYPAIRTLW
                     NSFHRVLPRREEFVGIANYSELIKDEIFWKAVSNTVTWACISPVIEVTVALLLALALY
                     AKVPGARFFRVAWFTPVLMSYIVVGILWMWIYNYDWGPLNTLLRAIGLGALAQPWLGS
                     PDTALGALILVTSWMWVGFNMVVLLAALHSLPKDVIEAAELDNCGWSAKLVFVIIPLI
                     RATLLNLVVLSFIGKMKIFDLVWITTKGNPLWSTETVSTYVYKRAFEWTTFDLGYPST
                     IATVWFVIVLATVLLLTWLFRTREKLEY"
     gene            7818..8636
                     /locus_tag="VF226_15415"
     CDS             7818..8636
                     /locus_tag="VF226_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7075428.1"
                     /translation="MPVLTRTLVLLALSLYTAFAAGPFVWAAMLSLRTTTEIHLNHFA
                     FPNPAHWEKFPDAWYNSNYATYFMNSIEVVIAAVVVLTIVGAMAAHCLARYRFRLNRV
                     IYFILFSTIIFPPQITIIALFQILVNYGLYNSKLGLTLVYVALQLPLTIYILESFFAR
                     LPADLFDAARMDGYSDWEIFWKVSLPIALPAVATTVILNFILLWNEFLFAVVLITDEA
                     NRTLPLGIQRFIGDTQEDIGMIATGLMIAIVPVIIVYAFFSEKLIQGMTAGAVK"
     gene            8647..9762
                     /gene="ugpC"
                     /locus_tag="VF226_15420"
     CDS             8647..9762
                     /gene="ugpC"
                     /locus_tag="VF226_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7075429.1"
                     /translation="MAIVRLERLTKIYERAQTPAVKDVSVEIGHGEFMVLLGPSGCGK
                     TTTLRMIAGLESISDGTLSIDGQIVNGVPAKDRDIAMVFQSYALYPHMTVWDNLAFGL
                     KRRSIPRDEIERRVRNVAATLGLSEFLKRKPHALSGGQRQRVALGRAIVRNPKVFLFD
                     EPLSNLDAALRVSTRGEIAALHRRLGATMIYVTHDQVEAMTLGTRICIMSNGRVAQVG
                     TPLDVYHNPASTFVAGFLGNPPMNLFPAVVTDDAGGRTLAIGSLKLTIPPERMAPLPP
                     GTSVTFGIRPEHVSAHGQGRPTEVPFSCEVVQVERLGAETLVISEIADSGARLVARSG
                     GDVALDLGDRVTLYADLSAAHVFDAAGNALRQQTETR"
     gene            9767..10450
                     /locus_tag="VF226_15425"
     CDS             9767..10450
                     /locus_tag="VF226_15425"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX7075430.1"
                     /translation="MAEPAQLRIRRAFPRPSRDEVAAFETAPTGWVVDAQGRRGALPH
                     WIRPLTKAKRFVGTALTVRTRPVDNLAPYAALKFAQPGDVLVVATDGSETASVLGDIL
                     LGMARNAGIVAAVTDGLVRDIEGIDRVGIPTFAKALSPNSPFKDGPGEVGFPVTIGGV
                     VIHPGDLIVGDIDGVVVVPCADVATTAAELKAIAAKEERMESVVAGGAKYPDWLDPIL
                     SSSRVRFED"
     gene            10496..11476
                     /locus_tag="VF226_15430"
     CDS             10496..11476
                     /locus_tag="VF226_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7075431.1"
                     /translation="MPPARPKVLYLSHATADLYALIKDVAGDRFEVITLERDDDDERC
                     RRIADCDAVICAATRLTKRHLDAAPKLRLVHHQGVGWQDTTDWQEIKRRGLPLAMTTA
                     GTTIGVAEHTVLLMLAAAKRLAFADAELRRGRWHVNALRGVSRELYGKTIGYVGMGRI
                     GQAVAERLRAFGCCGIYVDPAIRLPSDEEARLGLRNGMLDEVLTAADVLTLHVPLTAS
                     TRGLIGRNEIARLKPGAIIVNTARGGLIDEAALVEALKSGHVLAAGLDVFESEPPPPG
                     HPLLTLPNVVLTPHISAGTRDAMRQKMQAIFDNIEMFFATGDLKNRVVFP"
     gene            11535..12365
                     /locus_tag="VF226_15435"
     CDS             11535..12365
                     /locus_tag="VF226_15435"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7075432.1"
                     /translation="MNQRIVDCHAHLIDPARFPLSGEMGYKPKPEETGTREEFCRLLD
                     ENGVAHAVLVQLSGYGTDNSILLEAMRACPGRFKAIAVVDADISDAELERLSHAGVIG
                     VRFNLVSYDADHLLRPGTKHLLTRIKELGWFAQVFADDAQWLVAADLLRASGVRVLVD
                     HFGLTGPEGIRNPGFAAVCDLALDGIATIKLSAPFRISRRRPEYDDLDPIVELLLTKV
                     GPDSLIWGSDWPFPGVSPRLSYGDTLAPLARWLTSPEDREKVLWQTPARLFGFEEPKA
                     "
     gene            12362..13417
                     /locus_tag="VF226_15440"
     CDS             12362..13417
                     /locus_tag="VF226_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7075433.1"
                     /translation="MSIPCALRVAIAGAGMISRHHLLAWQKLGDDVEVVAICDPDSAR
                     ASERASEFGIAHVHASLDALFACEIIDALDVASPRQTHAAWVLAAAERRIPVLCQKPL
                     CPTLAEADDLIRKVGRRTRLMVHENWRFRPWFREARRWLDAGLIGDLLLARMTLLSSA
                     MLPDASGERPALVRQPFMAGEHRLMIAEVLIHHLDVMRYLCGPLRLVSARTLRSIPEV
                     KGETLAAMCLETADGCPVEVTGTMTAPGFHARPSDRLELIGSRASLVFDDWTLRLLGP
                     SPRKMRFDPDKGYQESFDAAIAHFVSCLRSDAPFETDPVDNIETLRLVEHAYWAAGQR
                     GPLSLFTGDRNGCCGKS"
     gene            complement(13503..13577)
                     /locus_tag="VF226_15445"
     tRNA            complement(13503..13577)
                     /locus_tag="VF226_15445"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13543..13545),aa:Asn,seq:gtt)
     gene            13838..14686
                     /locus_tag="VF226_15450"
     CDS             13838..14686
                     /locus_tag="VF226_15450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX7075434.1"
                     /translation="MDAFVFVAVLAAAAFHAGWNALLKIRLEPLTTVSLISAACGVVI
                     LPVAFFVDLPEPGAWPFIGMSLALHLVYYVALAEAYRFGDLSQVYPIARGTAPLITAL
                     GAAFWLGEPLGLVGWAGVILLGLGIVALSLRRKGGGLGRFEGRSVSFALLTAATIAAY
                     TLVDGTGARIGPSPAPYIVWLFLLDGAMMATFGLIRMPATMIQGAKSSWRMVLAGGAL
                     STAAYAIAIWAMTVAPIALVAALRETSVLFATLIGVLFLREPIYGLRIVAGCVVLAGI
                     VLLRLR"
     gene            complement(14694..16421)
                     /locus_tag="VF226_15455"
     CDS             complement(14694..16421)
                     /locus_tag="VF226_15455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX7075435.1"
                     /translation="MHGSELLLTLLGGVALLIWGVRMVRTGLTRAFGAPLRSVLNKAS
                     KNRLTAFGAGVGVAGVLQSSTATALLIASFVGRGLVALPVALAIMLGADVGTTLVAQV
                     FAFDIKWLWTVATISGVVLFSASHGDRSKNLGRIAIGVGLMLLALSVLGTVSGALRDS
                     PTVTMLLTALGTEPAIAVMVAAVLTWVSHSSLAVILLVLSLATGGVVGPVLALALVLG
                     ANLGGAIAPLTALTGSPVTARRVPLGNLLARGVVGIAIVPILPWAVTLMGYISDDPGR
                     LVLNFHTGFNILVAAIFLPLLTWLSRFVTWALPVPPDAPDRAVPQHLENSVLDTPSEA
                     LGCAMRETLRVGDIVLEMLRKSLAAIEGNDLKIVKELEKADDDVDALHEAIKLYLIRA
                     SKNELDDEESRRYVEILTFTTNLEHIGDIIDKNLMELANKKIKKRYSFSQEGLAELKR
                     FHARVVGNMRLALNVFATRDVTLARRLLMEKTALRAKEFEAADRHFARLKEGRAESIE
                     TSAIHLDIIRDLKRINSHLTAVAYPILEAAGELRESRLREKKREGQREEAPAREATLV
                     PTRQNERPA"
     gene            complement(16531..17436)
                     /locus_tag="VF226_15460"
     CDS             complement(16531..17436)
                     /locus_tag="VF226_15460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7075436.1"
                     /translation="MNLTHVLAFHRVATAGSFTVASRLAGVSQPTLSAQVRSLEQTMG
                     VALFERGGRGVRLTAAGEQLFASTRKLAEAMDEVGRLLATNRAPVRGHLRIAADSAVH
                     VLPVLAELKKAAASLSFSLHIDNSAAVMARVLSTEADIGVMARPTDNPRLFSTMIRQD
                     RLVLLVSRKDPLAQRKRARLTELAGRDLVVRERGSITREVAEARLESAGIRTGQVFDV
                     GTREAVAEAVAAGFGIGLAFASEVGRDPRLVAVRIQDADVDVAEYAICLSERRSIGTV
                     AKFLETARGLAIRNGWFTDHNSSGS"
     gene            17540..18682
                     /locus_tag="VF226_15465"
     CDS             17540..18682
                     /locus_tag="VF226_15465"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEX7075437.1"
                     /translation="MVGTPAVSETGDPLLLTPGPLTTAKSVKEVMVHDWGSRDSTFLR
                     INKEVLEALPAIVNASGTHVTVPMQGSGTFAVEAMLTTFIPATGKVLLLINGAYGHRA
                     KRICEIARRAVVVHETPEDTPPDLAEVERILATDKAITHVFIVHCETTSGILNPVAEV
                     GAIVERHGRRLLIDAMSAFGALPLDAARVKFDAVAASSNKCIEGVPGLGFVIAREAAL
                     AECKGNATTLVLDLHDQWQNFVKTGQYRFTPPIHVIVSFHQALAEFRQEGGQPGRGRR
                     YAENCKILIDGMRELGFTPLLPEELQAPIIVTFHMPKDPKFVFQTFYDSLKGRGYVIY
                     PGKLTVADSFRIGCIGRLDASHMRGALAAIADVLREMGISSLKSAA"
     gene            18696..19946
                     /gene="phnA"
                     /locus_tag="VF226_15470"
     CDS             18696..19946
                     /gene="phnA"
                     /locus_tag="VF226_15470"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEX7075438.1"
                     /translation="MNENHAARQVICNGRTYRFPKAPTVVICLDGSEPGYIEETIKAG
                     RAPTFERFMRDGANLFADSVIPSFTNPNNLSIITGRPPAVHGIAGNFFWDTAAQTEVM
                     MNDPKFLRAPTILKAFHDAGAKVAMVTAKDKLRLLLSDGLDYSTGRAIAFSSERADKA
                     TKQANGIDDVLEFVGLPLPDVYSAGLSEFVFAAGVKLIQTFRPDVMYLSTTDYIQHKV
                     GPGTKVANDFYAMIDGYLDQLDKAGAIVLATADHGMNDKHLPDGSPDVIYLQTLFDEW
                     LGKEAARVILPITDPYVAHHGALGSFATVHVPSSADIPSLLNRLRGIDGVDLALNKAE
                     ACERFELPPDRIGDIVVLSTKNKVLGTAPEKHDLSGLDEPLRSHGGLAEQRVPMISNR
                     KVEIPQGHRLRNFDAFAVALNHVV"
     gene            20041..20394
                     /locus_tag="VF226_15475"
     CDS             20041..20394
                     /locus_tag="VF226_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX7075439.1"
                     /translation="MYILANRPYGTLYIGVTSNLEARVSTHKQDLIDGFTRRYGIHRL
                     VYYEFMESMEDAIRREKRLKKWNRSWKFRLINRMNPEWNDLFDEFWGAVCNAPVDDER
                     LRIGERHRGSPPARG"
     gene            20456..>21052
                     /locus_tag="VF226_15480"
     CDS             20456..>21052
                     /locus_tag="VF226_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7075440.1"
                     /translation="MQDQVRKPKLPTAAHEKMRIAGKLVDADERLEVFNPYTGEVVGT
                     VPAARPDQVAEAFRIGNEYRSKLTRYDRQKILTRTAEILAARKEEIAHLITAESGLCL
                     KDSLYEVGRAYDVYLLAGQLAIRDDSEVYSCDITPHGKARRIYTSRTPVLGCISAITP
                     FNHPLNMISHKIAPAIATNNRVVAKPTEQTPLTALLLAD"
BASE COUNT         3881 a         6626 c         6579 g         3966 t
ORIGIN      
        1 cgtgtacgga gcgcgcgcga attggcctga gtcgagcccg gagatcatga tgtttcagag
       61 cttcgaagcc cccaccggcc tggaaaacgc ggtcgaccgc gtgaaacaac tccgtgcttt
      121 gatggcgcag ctcggggtgg acgcacttct ggtcccgcgg gccgacgagc accagggcga
      181 atatgtgccc gcctcggcgg agcgactgaa gtgggtcacc ggattttctg gatcggcagg
      241 cctcgctgtt attgcgcggc aatcggcagc tttattcgtg gacgggcggt acacggtaca
      301 ggcccgggcg caggtcgaca cgaagacatt cgagatcctg caggtgccgc aggctcgccc
      361 ctcggactgg ttgtcacaga agctgaaatc aggcgctgtc gtcggcttcg acccgcggct
      421 gcacacggtg agcgaggttg agcggctgac ggatgcgctg gcggagcgcg gcatcaagtt
      481 gagaccgctg agccgcaacc ccgtcgaccg gctctggggt gcgaatcggc cacccccgcc
      541 cgcaggcaag gtccggccgc accccttgca gtacgccggc cggtcggcag aggagaagat
      601 tgctgcactg caaaaaagtc tgaaagagga tcagcaggac gccgtagtgc tgaccttgcc
      661 ggactcgatc gcttggctgt tcaacatccg cggcagcgat gtcgcgcaca atcccgtgcc
      721 gctcgctttc gccatcgtgc cggcctcggg caagccggag ctgttcatcg cgcgcgagaa
      781 actcgacgcg gatgccaggg cgcatctctc cttcgcgaag gtaagcagcc ctgacaagct
      841 tgccgagcgg ctggctcgtc tgaaggccgc caacaagcgc gtgcggctcg atcccgacac
      901 ggcctcctgg tggtttgcgc gccagctcgg gggagcgcgc aaaatcgtgc gggggcagga
      961 tccctgcatt ctgcccaagg cccgcaagaa cgagaccgag atcaaaggcg cgcgcgctgc
     1021 gcatctgcgg gacggagccg ccttcgcgcg ctttctcgcc tggctcgatc gggaggctcc
     1081 gagcggaaag ctggacgaga tcagcgtggc gcggaagctc gaggagttcc gggccgagac
     1141 gcaggccctg aaagacatca gcttcgacac gatctcaggc gccggtccca acggggccat
     1201 cgtgcactac cgtgtcaccg aggcaacgaa ccgtcaactg aagcccggcg agctctacct
     1261 cgtcgactcc ggcggccagt acctcgacgg cacgaccgac atcacgcgca cgatcgccat
     1321 cggccagccg acggccgaga tgtgcgagcg ctacaccctc gtcctcaagg ggcatatcgc
     1381 gatcgccacg gcgcgtttcc ccaaaggcac gcgcggcatc gaaatcgatg ctttcgcccg
     1441 ccgtgccctg tgggagaaag gactcgattt cgaccacggg accggtcatg gcgtgggcag
     1501 ctatctttcg gtccatgaag ggccgcagtc gatctcccgc cgcggcatgg ctccgctcga
     1561 gccgggcatg atcgtctcca acgagcccgg atactacaag gagggcgcct acggaatccg
     1621 cctggagaac ctcgtcctcg tgacaggtcc ggaaatcccg cccggcggcg atcgcgagat
     1681 gctgagcttc gaaaccttga cgctcgcccc cttcgatcgg cgcctgatcc ggccggacct
     1741 gctgacgtca gccgagctgg attggctgaa cagctatcac gcccgcgtcg gcaagatcat
     1801 cggagccgag ctgggccccg acgaccgggc ctggctcgcc gccgctacgg cgccgatcgg
     1861 ttgagggggg ctttgccgga cagcgcagct cgtgagcagc ccatgtttcg ggcaggcggg
     1921 agcgagagtt gtcgatgacc ggcatgggca gctcctcgtc gttcaaaggc ccgtgcggac
     1981 gccgctgatc gtcggcagta tgtcatccgc tatggccgcg atgtcacccg aacggacaag
     2041 ccgtccggcc gccaccaggt cgggatgaag atggcggtcg tcattgaggg gcgggacctc
     2101 ggcgcggacg cgggcgcgga cctgttccag aatgcggctc gacttcagcg gtgcatggaa
     2161 atcacaaccc tgagcggctg cgagcagctc tatgccgatc acgctgacag cattctcgat
     2221 catcggcaac agacggcgcg cgccatgtgc cgccatggag acatggtcct cctggttggc
     2281 tgatgtcgga atcgaatcga tgctcgccgg gctggcacgc tgcttgttct cggaaaccag
     2341 tgcagccgca gtgacctgcg ggatcatgaa gccggagttc agccccggct ttggcgtcag
     2401 gaaggccggc aatcccgaca gcaccggatc gaccagcatg gcgatgcgcc gttcggccag
     2461 cgagccgatt tcgcaaatgg cgagcgcgat catgtccgcg gcgaaggcga ccggctcggc
     2521 gtggaagttg ccgcccgaaa gcgcgacgcc gtcctccgga aaaatcaatg ggttatcgga
     2581 gacgccgttg gcttcggttg caagcgtcga tgccgcctgc cgcaacacgt cgagcgccgc
     2641 gcccatcacc tgcggctgac agcgcaggca gtaaggatcc tgcacgcgct cgtcgccggt
     2701 cagatgcgag gccctgatct cggaacctgc aagcagtgca cgcaatgcat cagccgtttc
     2761 gatctggccg cggtggcggc gcagtgcatg gatgcgcgga tcgaagggcg catcggaacc
     2821 ccgggccgcg tccgtcgaca gtgcgccggt gacgagcgcc gagcggaaca ggttttctgc
     2881 ctcgaacagg gccgccagtg cgtatgcggt cgagaactgc gtcccgttga gcagcgccag
     2941 tccttctttc gcgccgagcg tcagcggcgc tagcccaact gaagccagcg cctcggcggc
     3001 accgacgttc ttgccgtcga ttgcaatttg gcccacgccg atcatggcag cagccaagtg
     3061 cgccagtggc gccaaatccc cggaggcgcc gacggagccc tgctgcggca ccaccggcgt
     3121 caggccgcgc gccagcatcg cttcgagcag ctcgacggtt gccaggcgca cgcccgaggc
     3181 gccttgcgcc aagctcgcca gcttcagcgc catcatcagg cgtaccactg gggcgggagc
     3241 cggatcgccg acgccggcag cgtgcgacag cacgatattg cgctgaagcg cttcaagatc
     3301 cgctggcgcg atacggacgc tggcaagctt tccgaagcct gtattgatgc cgtagaccgg
     3361 ctcgcctttc cgaacgatcg cggctactgc gtcggcggcc gcctgaagcc tgtggcggct
     3421 ggcgggagtg agtagcgaga catgcgcccc ctcatagatg gcgcgccatt gcgccagggg
     3481 gacgtcgccg ggaaccagct ccagtgcttt cgtcactgtc ctctccacat gggcggatgc
     3541 agcgggttga agcccatgca gtaaacgatc tcaagcggtc gttcgatgtt ccagatggcg
     3601 gcaaggtcat tctagtcgcg gccgccagga ccttggctgt tgcatcacgt tccgtagacc
     3661 gaagctgagc gtcgcatttc agccatcaat gctgatgcgc gtatggccgt tttgctggcc
     3721 gcctcccgcg gggtcgcctg tctgtgcgtc gagcggcgga cctggcgtag cagcgacgat
     3781 catgtttgtc cgccagatat ttcttggaga tgcctaccgc ctcgtccgcg cggttcgggc
     3841 cggggcttga cgaacggctt tccggcggcg caacaccgat tggagcgtgt tcagcaatca
     3901 tgactctccg caaagcccgc gccgggcgtc ccatgccaga ttgcatcctc tcgagatctc
     3961 ctcgcatggg cacacaggac acatcggagt ttcccaatgg gcgggccgaa accccaacgt
     4021 ctgatcgtcg gcatcagcgg cgcctcgggc gcggcttatg gcgtcgccgc gctcaggata
     4081 ttgcgggagc ttggcttcga aacacatctg gtcatgacgc gcgcggccgt gatcaccgcg
     4141 gcacacgagc tttcgctgaa agtccatgac attcaggagt tggcgagcgt cgttcaccgc
     4201 gtcgacgata tcgcggcctg catagcaagt ggttcgttcc agaccctcgg catgctggtg
     4261 gcgccctgct cgatccgcag cctgtccgaa atcgcctacg ggacgacgtc gaacctgctc
     4321 acgcgcgccg cggacgtcgt gctgaaggaa cggcggcggc tcgttctgat ggtgcgcgag
     4381 accccgctgc atctcggcca cctcaaagcg atgacccaag ctacggaagc tggcgcggtg
     4441 atcatgccgc cagtgcccgc gctttatgcc aagccaggct ccatcgacga catggtcagg
     4501 catagcgtcg ctcgggctct ggacctgttc gggatcgatg ccggcattgt cgatcgctgg
     4561 ggtgagcaac cgctgctggc gaccgcgaat actcggggca gcgccaaatc ggacgcgcca
     4621 tgatgacgtc gcgctgcgct gctagtccaa aattgctccc cgaacccgca agtacaattt
     4681 tttcgagtgc tggatatgcc ctttgacgag gcgcgcaagc tcgcggcgat caccacgctt
     4741 gagcgcatcg atcatggccc aatgctctag cctcagcgtt tcgagcagtt tcgggtcgac
     4801 gagcggataa agccgcatgg cccgtgacag ataggcgtaa tgcccgatgg cgtcgatgag
     4861 gaacttgttc ccgcaagcct cgaatatcgt tcggtgaaag acttcgttcg cctcgtcgat
     4921 acggcgcgga tcacgcgcgg caaccgcctt gtcgtgccgg cgttgaatgg cggtcagcgc
     4981 tgaaatgagc tcgggcgtgg ccggtagcgg catgcgctgt acggcgcgac tgtgcaacgt
     5041 ctcgcgcagt tcggcaagat cctccacctc ttcgctcgtg aaatctcgta ccgatgcacc
     5101 acgattgggt gcgcgcacga cgatgcccat gtgctcgagc ttgacaagtg cttggcgcac
     5161 tgcgtgacgc tttgccgaga atcgcgccat gagcgcatct tccaccagcc gctcccgcgg
     5221 gcgcagcgcg ccgaagagaa tgtcgaactc gagactgcgg acgatatcgg cgacggtcgt
     5281 ctcgccgccg gacgcctccg cgtgctctcg atcagccaaa tccaaccggc ccacggtata
     5341 atcccctgaa aacgggctgg ctccccccca gcaaggcgac tcactcgtca tcgccatgat
     5401 tatcaataat ctaattgacc ggcaactcgc tatcgactac ggtcgacagg caaggcggcg
     5461 cgaggccagc caggagaagg tctgcaaaaa ggggcgcgtg ttcgcgccca ggctgccatc
     5521 cagggaggaa gtgcgatgag atcgaagctg ctttcggtgc ttgccggcgc cgcgctggcg
     5581 tggaatgcgc ttgcagccca ggccgcggct cagacgaagg aaatcacatt ctggagccac
     5641 tgggccgcag agatgcccaa gcggaccttc gtcgaaaaag tcattgcaga tttcgaggcg
     5701 aaaaaccccg acatcaaaat caaggcgacc tggtacgaga aaacggccct ttacgcggcg
     5761 ctgaagacgg cgttgcgcgc ggggcaggca ccggacgtgt tctacgccga gcccgaccag
     5821 gtcgaatata tggatagtgg cttcctgctc gatctttccg agctcaactg ggcggcggtc
     5881 gagccctggg cgaaggaagc ctggatgtat aagggcaagc cctacggact tccgctcgag
     5941 gcttggacgg tcgagctcta ttacaataaa aagatgctcg acgagctcgg ggtgaagctg
     6001 ccggacaatc tgcagctctc acctgatgcc tttctcgata tggtgaagaa ggccaaggcg
     6061 aagggcatca cgccgatgtc gctcggcgct ggcgatcgac cgttcacggg tgcgcacctt
     6121 gttcacgagg cgcttctgaa gaagctcggt gtcgaggact attccaagct tctggcgggt
     6181 aagcttgcgt ggacggaccc acgtgtcgtc gagactctga cgtggatccg cagtttgacc
     6241 gacgcggggc ttctgccggc aacgttcacg acgatgaagc tcgccgaggc gcatatctat
     6301 ttccatacaa accccggcgc gctcactttt ctgaacggaa gttggtacac ctcgcgtgca
     6361 ttcaatcccg tcgacaaggg cggccagcca gaggatttcc agctcggcat catgcaattc
     6421 ccgtctgttc cgggtgctgt ctgcaacgag tgccgcacca tagcagtcgg cggcagctac
     6481 gtggccaacg cggagacgga gcataaagct gaggtcatta aattcctgaa ctcgtttctc
     6541 tcgccagaag ttggcaatga gtggcttgaa aacgtgaaag ttcagaccgg catcaagagc
     6601 gatccatcga agatgaacga caagcaggcg tcggagtact tcgcgatgat cgccaagacc
     6661 aatgccggcg cgaaatatca cttcggactt cccattcagg tgatgaccgg gaagccgaaa
     6721 gaggtgttca cgcaggtgtt caacaacgcc ttcccggccg gcaatatcag tgtcgaggac
     6781 gcagtgaagc agatggacgc cgccaactga tcacagcggg gtggggcagc ggccgggctg
     6841 gcgcgctggg gggcgcgaaa gcccggccgc atctgtcgaa agatcggaat cgatcatgcc
     6901 tgcatttcgg gccaaggagc tgaaaggcga cgtgcgtcag ccgtggggaa cgattgtcgc
     6961 gttccttttg cccgcactga cattctacgt cgcgctgacg gcctatccgg cgatccgcac
     7021 attgtggaac agctttcaca gagtattgcc acgccgcgaa gagttcgtgg gcatcgcgaa
     7081 ttacagcgag ctcataaagg atgaaatctt ctggaaagcc gtcagcaata cggtgacctg
     7141 ggcgtgtatc tcgcccgtga tcgaggttac ggtggccctt ctgcttgcgc tcgcgctcta
     7201 cgccaaagtg cccggcgcgc gcttcttccg tgtcgcctgg ttcacgcccg tgctgatgtc
     7261 ctacatcgtc gtcggcatcc tgtggatgtg gatctacaat tacgattggg gaccgttgaa
     7321 tacgctgttg cgcgcgatcg gcctcggcgc gctcgcccag ccttggctcg gcagccccga
     7381 caccgcgctc ggcgcactca ttctcgtgac aagctggatg tgggtcggct tcaacatggt
     7441 cgtgctgctc gcggccctgc attccctgcc caaggacgtg atcgaggcgg cggagctcga
     7501 caattgcggc tggtccgcaa agctcgtgtt cgtcatcatt ccactcattc gtgcgacgct
     7561 gctgaatctc gtcgtcctgt cttttattgg aaagatgaag attttcgacc tggtgtggat
     7621 caccaccaag ggcaatccac tgtggtcgac ggaaactgtt tcgacctatg tctacaagcg
     7681 cgccttcgag tggacgacat tcgatctcgg ctatccctcg actattgcaa ccgtctggtt
     7741 tgtcatcgtg ttggcgacgg tgcttctcct gacatggctg ttccgcacgc gcgagaagct
     7801 cgagtattga ggcaccaatg cccgtcctga cccgcacgct cgtgctgctg gcgttaagcc
     7861 tttacacagc gttcgccgcg gggccgttcg tctgggcggc aatgctgtcg ctcaggacga
     7921 caaccgagat ccacctcaac catttcgcgt ttcccaatcc cgcgcactgg gaaaaattcc
     7981 ccgatgcctg gtacaattcg aattacgcga cttatttcat gaattccata gaggtggtga
     8041 ttgcggcggt cgttgtgctg accatcgtcg gagccatggc ggcgcattgc ctggcgcgct
     8101 accggttccg gctcaatcgt gtcatttact tcattctttt ctcgaccatc atcttcccgc
     8161 cgcagatcac gatcatcgcg ctgttccaga tcctcgtgaa ctacggcctc tacaattcca
     8221 agctcgggct gacgctcgtt tacgtggcgc tgcaattgcc gctgacgatc tacattctgg
     8281 agagcttctt cgcccgcctt ccggccgatc tcttcgatgc ggcgcgtatg gacggctatt
     8341 cggactggga gatcttctgg aaggtatcgc tgccgattgc gctgcctgca gtggcgacga
     8401 cggtcattct caatttcatt ctgctctgga acgagttcct gtttgcggtg gtgctcatca
     8461 ccgatgaagc caatcgcacc ttgccgctcg gcatccagag gttcatcggc gacacgcagg
     8521 aggacatcgg aatgatcgca accggcctga tgattgcgat cgtcccggtg atcatcgtct
     8581 acgcgttctt ctccgaaaaa ctcatccagg gcatgaccgc cggcgctgtg aagtaggtga
     8641 ggggtaatgg ccatcgtacg gctggagcga ctgaccaaaa tctacgaacg ggcgcagaca
     8701 cccgcggtaa aggacgtgtc ggtggaaatc ggacatggcg agttcatggt cctgctcggg
     8761 ccgtccggct gcgggaaaac gacgacgctg cgcatgatcg ccgggcttga gtcgatttcc
     8821 gacggcaccc tttcgatcga cggtcaaatc gtgaatggcg tccccgccaa agatcgcgat
     8881 atcgcgatgg tttttcagtc atacgcgctc tatccgcata tgactgtgtg ggacaatctc
     8941 gcctttggac tgaaacgccg gtcgattccg cgcgatgaga tcgagcggcg tgtcaggaac
     9001 gtcgccgcaa cgctcggcct ttccgagttc ctgaaacgca agccgcatgc gctttccggc
     9061 ggacaacggc agcgagtcgc actggggcgc gccatcgttc ggaatccaaa ggtcttcctg
     9121 ttcgacgagc cgttgtccaa tctcgacgcg gcgttgcggg tgtcgacccg cggtgagatt
     9181 gcggccctgc atcggcgtct gggcgcgacg atgatctacg tgacgcacga ccaggtcgag
     9241 gcgatgacgc tcggcacgcg catctgcatc atgagcaatg gccgcgtggc gcaggtggga
     9301 acaccgctcg atgtctatca caatcccgca agtacgttcg tcgccggctt tctcggcaat
     9361 ccgcccatga acctgtttcc agcggtggtg acggacgatg cgggcggccg cacgctcgcg
     9421 atcggtagtc tcaagctcac cattccgccg gaacgcatgg cgccgcttcc gccgggcacg
     9481 tctgtgacgt tcggcatccg ccccgagcac gtcagcgctc atggccaggg caggccgacc
     9541 gaagttcctt tctcctgcga ggtcgtgcag gtcgagcgcc tcggcgccga aacactcgtc
     9601 atctccgaga tcgcggactc cggagcccgg ctcgttgcgc ggtcgggcgg tgatgtcgcg
     9661 ctcgacctgg gcgatcgcgt gacgctttac gcagaccttt cagccgcgca cgtcttcgac
     9721 gccgccggca acgcacttcg tcaacaaacc gaaacgaggt aacggcatgg ccgagccagc
     9781 acagttgcgc atccgccgcg catttccgcg tccctctcgg gacgaggtgg cagcattcga
     9841 gaccgcgccg acggggtggg tggtggacgc gcagggccgc cgtggcgcgc ttccacattg
     9901 gatccggccg ctgacgaagg cgaagcgctt cgtcggcacg gccttgaccg tgcggacgcg
     9961 gcccgtcgac aatctcgcgc cctacgccgc gctcaagttc gcccagccgg gcgacgtgct
    10021 tgtcgttgcc accgacggca gcgagaccgc ttcggtcctc ggggacatcc tgctcggaat
    10081 ggcaaggaac gccggcatcg tcgcggccgt caccgacggc ctcgtccgcg atatcgaagg
    10141 tatcgaccgc gtcggcattc cgacgttcgc gaaggcgctg tcacccaact ctccgttcaa
    10201 ggatggacca ggcgaagtcg ggtttccggt gaccatcggc ggcgtcgtca tccatcccgg
    10261 cgatctcatc gtgggtgaca tcgacggcgt ggtggtagtc ccgtgcgccg acgtcgcgac
    10321 aaccgcagcc gaactcaaag cgatcgccgc caaggaggaa agaatggagt cggtcgtggc
    10381 cggcggtgcc aaatatcccg attggcttga ccctatcctt tcctcctcgc gcgtacgctt
    10441 tgaggactga attcattcct gccctggagc gttatcggtc tcaatgaaag ccgccatgcc
    10501 acccgcgcga cccaaagtgc tctacctgag ccacgccacc gccgatcttt atgcgttgat
    10561 caaagatgtg gccggcgatc gcttcgaagt catcacgctt gagcgcgacg atgacgacga
    10621 gcgatgccgc cggattgcgg attgcgatgc ggtgatctgc gccgccacac ggctgacgaa
    10681 gcgccatctc gacgccgccc ctaagctgcg gctggtgcat caccagggcg tcggctggca
    10741 ggacacgaca gactggcagg aaatcaagcg ccgcgggctg ccacttgcca tgacgacggc
    10801 aggaacgacc atcggcgttg ccgagcacac ggttctcctg atgctcgctg ccgccaagcg
    10861 ccttgccttc gccgatgcgg agctgcgtcg cggccgctgg catgtgaatg cgctgcgcgg
    10921 tgtctcgcgc gagctctatg gaaagacgat cggctacgtc gggatgggac ggatcggtca
    10981 ggccgtcgcc gaacgcctca gggcctttgg ctgttgcggc atctacgtcg atccggctat
    11041 ccgcctgcct tcggatgagg aggcgcgcct cggtctccgc aacggcatgc tcgacgaggt
    11101 tctgactgca gccgacgtgc tgacgctgca cgtgccgctg acggcatcaa cccgcgggct
    11161 cataggcagg aacgagatag cccgcctcaa gcccggtgca atcatcgtca acaccgcgcg
    11221 cggcgggttg atcgacgagg ctgccttggt cgaggcctta aagtcgggcc atgtcctggc
    11281 tgcaggtctc gacgttttcg agtcagagcc gccacccccc ggacatcccc tcctgacgct
    11341 gccgaatgtt gttctgacgc ctcacatttc cgccggcacg cgcgatgcca tgcggcaaaa
    11401 aatgcaggcc attttcgaca acatcgaaat gttcttcgca accggcgacc tgaagaaccg
    11461 ggtggtgttt ccgtaatttg ccgataagca gagcggaccg cgttgtcggc gatggatgcc
    11521 cttgaggtga gcttatgaac cagcgaattg tcgactgcca cgcccatctc atcgatccgg
    11581 cccgctttcc cctcagcggc gagatgggct acaagcccaa gcccgaggaa accggaacgc
    11641 gagaggaatt ctgccggctg ctcgacgaga acggtgtggc gcatgccgtg ctggttcagc
    11701 tcagtggata cggcacggac aattccatac ttcttgaggc gatgagggcc tgcccgggcc
    11761 ggttcaaggc catcgctgtg gttgatgccg acatttcgga cgccgagctg gagcggctct
    11821 cgcatgcggg cgtcatcgga gtccgcttca atctcgtcag ctacgatgcc gaccatctgc
    11881 ttcgaccggg cacgaagcat ttgctcaccc ggattaagga gctgggatgg ttcgcgcagg
    11941 tttttgcgga cgacgcgcaa tggctggttg cggctgatct gttgcgcgcg agcggcgtac
    12001 gcgtgctggt cgaccatttc ggcctaaccg gcccggaagg cattcgcaat cctggcttcg
    12061 ctgcggtttg cgatttggcg ctcgatggca tcgccacgat caagctgtcg gcgcccttcc
    12121 gcatctcgcg aaggcgcccc gagtacgacg acctcgaccc gatcgtcgag ctcctgctga
    12181 caaaggtcgg gcccgactcg ctgatctggg gctctgattg gccgttcccc ggagtttcac
    12241 cgcgcttgag ctatggagac acgctcgctc ccctcgcgcg ttggctcaca tcccctgaag
    12301 atcgcgagaa ggtgctttgg cagactccag ctcggctttt cggattcgag gagcccaaag
    12361 catgagcatc ccgtgcgctc tcagggttgc tatcgccggc gcaggcatga tcagccgcca
    12421 ccatctcctg gcttggcaga agctggggga cgacgtcgag gtcgtcgcaa tctgcgaccc
    12481 tgattcggcg cgtgcaagcg agcgtgccag cgagttcggc atcgcgcacg tccacgccag
    12541 cctcgatgca ctgttcgctt gcgagatcat cgatgcgctc gacgtcgcca gtccgcgcca
    12601 gacgcacgcg gcttgggttc tcgcggcggc ggagcggcgt attcctgttc tgtgccagaa
    12661 gccgctgtgc ccgactttgg ccgaagccga cgatctcatc cgaaaggttg gccggcgcac
    12721 ccgtctcatg gtgcacgaga actggcggtt cagaccctgg tttcgcgagg cccggcgctg
    12781 gctcgacgcg ggcctgatcg gcgatttgct cctggcgaga atgactcttc tgtcctccgc
    12841 tatgctgccg gacgcttctg gtgagcggcc cgcgcttgta cgccaaccgt tcatggcagg
    12901 tgagcaccgg ctgatgatcg cagaggtgct gatccatcac ctcgatgtca tgcgctatct
    12961 ctgcggtccc ttgcggctgg tctccgccag gaccttgcgc tccattcccg aagtgaaggg
    13021 ggagacgttg gccgcgatgt gtctcgaaac ggctgacggc tgccccgtag aggtgacggg
    13081 aacaatgacc gcgccaggat tccatgcccg tccgtcagac cggctcgagc tcatcgggtc
    13141 ccgtgcgagc ctcgtcttcg atgactggac tcttcggctg cttggcccat caccgcgcaa
    13201 aatgcgtttc gatccggata agggctatca ggagagtttc gacgcagcga tcgcgcactt
    13261 cgtctcgtgt ctcagatcgg atgcaccatt cgaaacggac ccggtcgaca atatcgagac
    13321 gcttcgcttg gtcgagcatg cgtattgggc cgcaggtcag cgcggaccat taagtctttt
    13381 tacgggcgat cgaaacggct gttgtgggaa gagctgaatg cggctctgaa cgcgccgggt
    13441 ctagcgctgg cttggcgtgg tgagagcatg cgcgggatgt ctctattggc gcaacgctca
    13501 gatggctccg cgggtaggat tcgaacctac gaccaaccgg ttaacagccg gttgctctac
    13561 cgctgagcta ccgcggaatg gaccaccttg agacccgggc tcatagcaga aagaggtcgc
    13621 gagggctagc cccctcagcg cattgccgcg ggggtccgga ggatgcctgc cgattggacg
    13681 cccgtaaccg attccgtacg caccggctcc gtcacgagga cgagcgcctg agcccaattc
    13741 tcttgcaaaa gagatgcact tccatgtcag ttccgctagg agtgcgccgc ctgggccgaa
    13801 ggcggatgcg ccgcgggcta cgttggaggc ttgaagcatg gacgcattcg tcttcgtggc
    13861 agtgctggct gccgccgctt tccacgcggg atggaatgcg ttgctcaaga tcaggctcga
    13921 gccgctgacg accgtcagcc tgatctctgc ggcctgtggc gtcgtgatcc tgccggtcgc
    13981 gttcttcgtc gatctgccgg aaccgggggc gtggccgttc atcgggatgt ccctggcgct
    14041 tcacctcgtc tactacgttg cgctggcgga ggcttatcga ttcggggatc tctcgcaggt
    14101 ctacccgatc gcacggggga cggcgccgct catcacggcg cttggcgctg ccttctggct
    14161 cggcgagccg ctgggccttg tcggatgggc gggcgtcatc ctgctcggcc tcggcatcgt
    14221 ggccctgtcg ctgcgccgga agggcggcgg gctcggccgc ttcgagggac ggtccgtctc
    14281 attcgcactg ctgaccgccg cgaccatcgc cgcctacacc ctggtcgacg gcaccggcgc
    14341 ccgcatcgga ccatcacccg cgccatacat cgtttggctg ttcctgctcg acggcgcaat
    14401 gatggccacc ttcggattga tccgcatgcc ggctaccatg atccagggcg ccaagtcgtc
    14461 ctggcgcatg gtgctcgctg gcggagcgct gtcgacggct gcctacgcca ttgccatatg
    14521 ggccatgacg gtcgccccga tcgcgctcgt cgccgctttg cgcgaaacga gcgtgctctt
    14581 cgcaacgctc atcggcgtcc tcttcctgcg tgagccgatt tacggcttgc gcatcgtggc
    14641 cggctgcgtg gtgctcgccg gcatcgttct gctgcgactg cggtaggcgg agctcaggcc
    14701 ggccgctcgt tttgccgggt cggcacgagt gtagcctcgc gggcaggcgc ttcttcgcgc
    14761 tggccctccc gcttcttctc gcgcagccgg ctttcgcgca actcgcctgc tgcctccagg
    14821 atcgggtagg cgacggcggt caggtgcgag ttgatgcgtt tcaggtcgcg gatgatgtcc
    14881 aggtggatgg cactggtttc gatcgattcc gcacggccct ctttgagccg ggcgaaatgt
    14941 cggtctgcgg cctcgaactc cttggctcgc aaggccgtct tttccatcag caaccgccgc
    15001 gccagcgtca cgtcccgcgt cgcaaagaca ttgagcgcaa gccgcatgtt gccgaccacc
    15061 cgggcgtgga accgcttcag ttccgccagt ccttcctgag aaaacgagta ccgcttcttg
    15121 atcttcttgt tggcaagctc catcaggttc ttatcgatga tgtcaccgat gtgctcgaga
    15181 ttggtcgtga acgtcaggat ctcgacgtag cggcgcgact cctcgtcgtc gagctcgttc
    15241 ttcgacgcgc ggatgagata gagcttgatc gcctcgtgca gagcgtcgac gtcgtcgtca
    15301 gccttttcga gctcctttac gatcttgaga tcgttgccct ctattgcggc gagcgatttg
    15361 cgcagcatct ccaggacaat gtcgccgacg cggagtgtct cgcgcatggc gcagccgagg
    15421 gcttccgacg gcgtgtcgag cacactgttc tcaaggtgtt gcggcacggc cctgtcgggg
    15481 gcgtccggcg gcaccggcag cgcccacgtg acgaaccgcg acagccaagt gagcagcggc
    15541 aggaagattg cggcaacgag aatgttgaag ccggtgtgga agttgagcac gagccggccc
    15601 gggtcgtcgc tgatgtagcc catcagtgtc acggcccatg gcaagatcgg tactatcgcg
    15661 atcccgacga caccgcgcgc aagcagattt ccgagcggaa cccgtcgggc tgtcacgggc
    15721 gacccggtga gcgccgtgag cggggctatc gcgccgccca ggtttgcgcc gagcacaagg
    15781 gccagggcga gaacaggtcc gacgacgccg ccggtcgcaa gcgacagcac gagcagaatg
    15841 acggcaaggc tggaatgcga cacccacgtc agaactgcgg ccaccatgac cgcaatggcc
    15901 ggctcggtgc cgagtgcagt cagtagcatc gtgacggtgg ggctatcgcg cagggccccc
    15961 gagacggtcc ccagcacgga gagcgccagc agcatgagac ctaccccgat cgcgatacgg
    16021 ccgaggttct tcgaccggtc gccatgcgaa gcactgaaca gcaccacacc gctgatcgtg
    16081 gcgaccgtcc atagccattt gatgtcgaac gcgaagacct gcgcaacgag cgtggtgccg
    16141 acgtcggcgc cgagcatgat cgcgagcgcg acggggagcg ccacaagtcc gcgtccgaca
    16201 aacgaggcga tcagcagggc cgttgcggtg gaactctgca ggacgccggc cacgccgacg
    16261 cccgcgccga aggcggtgag ccgattcttg gaggccttgt tgagcacgct ccgtagcggc
    16321 gcgccgaacg cgcgggtcag gccggtacga accatgcgca cgccccagat caggagcgcg
    16381 acgccgccca gcagtgtcag gagcagctcg cttccatgca tttctttttt gtctccgaat
    16441 aaaaacgcga cgccatcagg atcagaaaca aagattcgtg gcggtgtcgc gagccagcca
    16501 gcatactccc gcttacttta aaagcctagc tcacgacccg gaactgttgt ggtccgtaaa
    16561 ccaaccattc cgaatggcga ggccgcgggc cgtttccaga aacttggcca ctgtgccgat
    16621 gcttcgtcgc tccgacaggc aaatcgcata ttctgcaacg tccacgtcgg catcctggat
    16681 ccgtactgcg accaaccggg gatcgcgtcc gacctcgctc gcaaaggcaa gcccgatgcc
    16741 gaaccccgcg gctacggctt cggcgacggc ttcccgtgtg ccgacatcga aaacctgtcc
    16801 cgtcctgatg ccggcggatt cgaggcgcgc ctcggcaacc tctcgcgtga tcgagccgcg
    16861 ctcccggacc acgaggtccc ggccggcgag ctccgtcaac cgcgcccgct tgcgttgcgc
    16921 gagcggatcc ttgcggctga ccagcaacac gagcctgtcc tgccggatca tcgtcgagaa
    16981 aaggcgcgga ttgtcggtcg gccgggccat cacgccgata tcggcctcgg ttgacagaac
    17041 ccttgccatt acggcggccg agttatcgat gtgcagcgaa aaactcagcg atgccgccgc
    17101 cttcttcaac tcggccagaa ccggcagaac gtgcaccgcg ctgtctgccg cgatccgcag
    17161 gtgaccgcgc acgggtgcgc ggttcgtagc gagcaggcgg ccgacctcgt ccatggcttc
    17221 ggccagcttc cgcgtcgacg cgaaaagctg ctcgccagca gcggtgagcc ggacgccccg
    17281 gccgccgcgc tcgaaaagcg cgacgcccat cgtctgctcc aatgaccgga cctgtgccga
    17341 cagcgtcggc tggctaacgc cggcgagtcg cgaagcgact gtgaagcttc cggccgtcgc
    17401 gacgcgatgg aaggccagca cgtgcgtgag gttcatagat aaaatctatg tttccgatcg
    17461 aaggcgtata ttggatcaat atataggccc gcgctagtct cggcgcaaac gaagtctcag
    17521 ccactggagg ccagattaca tggtgggcac acccgcggtc tcggagaccg gcgacccgct
    17581 tctccttacc cccggcccgc ttacgacggc gaagtcggtc aaggaggtca tggttcacga
    17641 ttggggctct cgcgattcca catttctgcg gatcaacaag gaggtcctgg aagctctgcc
    17701 ggcgatcgtg aacgcgagcg gcacgcacgt cactgtgccg atgcagggct ccggcacatt
    17761 cgccgtcgag gcgatgctga cgacgttcat cccggccacc ggcaaggtgc tgctgctcat
    17821 caatggcgcc tatggccacc gcgccaagcg catctgtgag atcgccaggc gcgcggtggt
    17881 ggtgcacgag acgccagagg atacgccgcc ggatctcgcc gaggtcgagc gcatcctcgc
    17941 caccgacaag gcgatcacgc acgtcttcat cgtccattgc gagacgacca gcggcatcct
    18001 taatcccgtt gccgaggttg gcgccatcgt cgagcggcat ggacgccggc tgctcatcga
    18061 tgccatgagc gccttcggtg ccttgccgct cgatgcggcc cgggtcaaat tcgatgccgt
    18121 tgccgcctcc tcgaacaagt gcatcgaggg cgtgccaggg ctcggattcg tgatcgcaag
    18181 agaggcggca ctcgccgaat gcaaaggcaa cgccacgacg ctggtgctcg atcttcacga
    18241 ccagtggcag aatttcgtga aaaccggcca gtatcgcttc acgccgccta ttcacgtgat
    18301 cgtctcgttc caccaggcgc tggccgagtt ccggcaggaa ggcgggcagc ccggccgcgg
    18361 ccgccgctac gcggaaaact gcaaaattct catcgacggt atgcgggagc tgggatttac
    18421 gccgttgctt ccagaagagc tccaggcgcc gatcatcgtc accttccata tgccgaaaga
    18481 cccgaaattc gtttttcaga cattctacga ttccctgaaa ggccgcggtt atgtgatcta
    18541 tcccggcaag ctcaccgtcg cggacagctt ccgcatcggc tgcatcggca ggctcgatgc
    18601 cagccacatg cgcggcgcat tggccgcgat tgcggatgtg ctaagggaaa tgggaatttc
    18661 cagcctgaaa tcggcagcat gaggagttga aatcgatgaa tgaaaatcac gctgcccgtc
    18721 aggtgatctg caacggtcgg acctacaggt tcccgaaagc gccgacggtg gtgatctgtc
    18781 tcgacggttc cgaaccgggt tatatcgagg agacgatcaa ggccggccgg gcaccgacgt
    18841 tcgagcgctt catgcgcgat ggcgccaacc tgttcgccga cagcgtcatt ccaagcttca
    18901 ccaatccgaa caatctgtcg atcatcactg gccgtccacc tgccgtgcac ggcattgccg
    18961 gcaatttctt ctgggacaca gccgcccaga ccgaagtgat gatgaacgat ccgaaattct
    19021 tgcgggcgcc gacgatcctc aaggcattcc acgatgccgg cgccaaggtc gcgatggtga
    19081 ccgcgaaaga caagctgcgg ctgctgttgt cagacgggct cgattattcg accggccgcg
    19141 ccattgcctt ttcctccgaa cgcgctgaca aggccacgaa acaggcgaac ggcatagacg
    19201 acgtactcga gttcgtcggc ctgccgctgc cggacgttta ttccgcaggg ctgtcggagt
    19261 tcgtgttcgc cgccggggtg aagctgatcc agacgttccg gcccgacgtt atgtatttgt
    19321 cgaccaccga ctacatccag cacaaggtcg gcccgggcac gaaggtggcc aacgattttt
    19381 atgcgatgat cgacggctac ctcgatcagc tcgacaaagc gggtgctatc gtccttgcca
    19441 cagccgacca cggcatgaac gacaagcatc tcccggacgg ctcaccggac gtcatctact
    19501 tacagacgtt gttcgatgaa tggctcggca aggaagcggc tcgtgtcatc ctgccgatca
    19561 cggatcctta tgtggcgcac catggcgcgc tgggctcgtt tgcgaccgtg cacgttccga
    19621 gcagtgccga tattccaagc cttctgaacc ggctgcgcgg catcgacggc gtcgaccttg
    19681 ccttgaacaa ggcggaggcc tgcgaacgct tcgagctccc gccggatcgc atcggcgata
    19741 tcgtcgtgct gtccaccaag aacaaagttc tcggcactgc gcccgaaaag cacgaccttt
    19801 ccggcctgga cgagcccctt cggtctcatg gcggcctggc agagcagcgc gtgcccatga
    19861 tttcgaaccg caaagtcgaa attccacaag gccatcggct gcgcaatttc gatgctttcg
    19921 ctgtcgcgct gaaccatgtg gtttgaatga gctattcacc ccaatccgtc attcccgcga
    19981 aggcgggaat ccgtgatgcg tagatgcccg gatccggttt atattgtgaa gcgaccgtgc
    20041 gtgtacattc tggcaaacag gccttatggc accctctaca tcggcgtgac gagcaacctg
    20101 gaagcgcgtg tttcgactca caaacaagac cttatcgacg ggttcacgcg gcggtacggc
    20161 attcatagac tggtctacta cgaattcatg gagagcatgg aggatgcgat ccggcgggaa
    20221 aagcggttga agaagtggaa tagatcttgg aaattcaggc tgattaaccg catgaatccg
    20281 gagtggaacg atctgtttga cgaattctgg ggcgccgttt gcaatgcgcc cgtagatgac
    20341 gagaggttgc gaattgggga gaggcatcgt ggatccccgc ctgcgcgggg atgacgttga
    20401 gaatgctcaa agctctttcc caaaggtaag atgacagaaa tactcgagga cccggatgca
    20461 agaccaagtc aggaaaccca agctgcccac cgccgcgcac gagaagatgc gcatcgcggg
    20521 caagctggtc gatgccgatg agcggctgga ggtgttcaac ccctatacgg gcgaggtggt
    20581 cggaacggtg ccggcggcgc gaccggacca agtggccgag gcgttccgca tcggaaacga
    20641 ataccgctcg aagctgacac gctacgaccg gcagaaaatc ctgacgcgca cggcggagat
    20701 cctggccgcg cgcaaggagg agatcgccca tctcatcacg gcggagtccg gcctatgcct
    20761 caaggactcg ctctatgagg tgggtcgcgc ctacgacgtt tatcttctcg caggtcagct
    20821 cgcgatccgc gacgatagtg aagtctattc ctgcgatatc actccgcacg gaaaggcgcg
    20881 gcgcatctat acctcgcgca cgccggtgct cggctgcatc tccgcgatca cgccgttcaa
    20941 ccatccgctg aacatgatca gccacaagat cgcgccggcc atcgccacca acaatcgcgt
    21001 ggtcgcgaag ccgaccgagc agacgccgct gacggcgctg cttctcgccg at
//
LOCUS       DASVDU010000240         7365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1624451, whole genome shotgun sequence.
ACCESSION   DASVDU010000240 DASVDU010000000
VERSION     DASVDU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624451"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF226_15485"
     CDS             <1..299
                     /locus_tag="VF226_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075441.1"
                     /translation="LTVGFFAKFYAVAGGVGAAAWPAIFALVAGSVIGLFYYLRVIVI
                     MATPVAEAEAVEFETVPWTGGATLAALTFILIWLGLYPAPLISLIRLTALQVGN"
     gene            complement(587..1489)
                     /locus_tag="VF226_15490"
     CDS             complement(587..1489)
                     /locus_tag="VF226_15490"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX7075442.1"
                     /translation="MSLERFRRSRMVVLPQSAMAYQAARAMADNHIGSVLVGGPKGLV
                     GMVTDRDLALAVLGGDLDPKKTRLSEVMSEGVISCDIGADIGEVVRLMRDEGVRRIAI
                     TENERLVGLVTLDDLVVDGSVGIEDLRSIVSAQLEVEAPHKPAGTLHPEAGAKLQAAG
                     RARALMRAQARAEATYHRVVTTVADAAGLDRDRAERALLLGACMLCRRLVPQEAEDLI
                     AQLPSMLQPRLEKCLDGPDRAVTADAIRDELARSLGLDREQATITMKSVFKAIGETVS
                     AGQIEEVRGQLPEEMKHLFPETQA"
     gene            complement(1818..3302)
                     /locus_tag="VF226_15495"
     CDS             complement(1818..3302)
                     /locus_tag="VF226_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX7075443.1"
                     /translation="MAARIRAHDWGSTALGPMESWGHPLKTAVQLMLGAAQPTYVAWG
                     PELVSLYNDGYIPILGAKHPSALGRPLREVWADIWDDLQPLVEATLTGQGQHCVDCPL
                     EISGRPERPVSWFTFSWTPLRNDAGDVEGFYCATTETTDRILAERALHESEQRLRLAI
                     EAAEIGIFEIEVETGRVRYSSELARLLGFPEVRETDVEAAFARVHREDVAHVRRLYNE
                     ALSPSGSGHLMMDFRFVRPGGEVRWMTWNGRLQSQHRDGELSAPRIVGVCIDITERKR
                     HEEHIQLLMREVNHRSRNMLALVMAIARQTFRAEHRQALDLFQGRIQALSTSLDLLVR
                     NEWRGVELSDLVRSQLSHFSNLFGNRIRWGGPTLMLSPTAAEAIGMVLHELATNAGKH
                     GALKDDAGTVRLDWQIQSDETGAAIFELSWTEAAGTAVTPPTRRGFGSAVIIDAIKAQ
                     LGAEVNLAYPPSGIVWQMRCPLGAIVGNSVGASPGAAPAAPLII"
     gene            complement(3630..4391)
                     /locus_tag="VF226_15500"
     CDS             complement(3630..4391)
                     /locus_tag="VF226_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7075444.1"
                     /translation="MARLKLTFLGTRGGIILRSPRHLRHSSLLVESERTRVMIDCGSD
                     WLHQLNSLAPTAIVLTHAHPDHAGGLAAGAPCPVYATSETWAALGRFPVEAKRTIPPG
                     NPLLIDHLTFQAFPVEHSLLAPAVGLRICAEGSCIFYAPDVAEILDRRGALSGVDLYI
                     GDGATVRRSMVRRRNGALIGHASIAAQLGWCKDAAVRQAIFTHCGSQIVRDKSQEMET
                     LVRRLGREHGVAARIAYDGLQLSIGGKPSAGTLSV"
     gene            complement(4611..7268)
                     /locus_tag="VF226_15505"
     CDS             complement(4611..7268)
                     /locus_tag="VF226_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075445.1"
                     /translation="MSRSGHDNVAVRPTQLVTSATLAAVAATPAAAHGFGERYELPLP
                     LELYVYGAAAVVALSFLAFGLFVHRAPAPRERSPKNLLTHPVGRLLSHWTVVLGMRLA
                     ALALFLVVIVAGFLGDQNPYRNIVPTLVWIIWWVGFAFLSAFIGNVWALVNPWTTAFD
                     GVHLALRRLGWEGAAEPVLRYPGALGVWPASIFLLAFAWVELIDPNAASPAHIAKLAI
                     AYSILTWAGMALFGRDIWLKHCEVFSLFFGTFARFSPLEVKKGRVLVRPFGSGLVESP
                     TVSTSMVAFTLLLLATVLYDGLIGTGEWALFESALQAHAHDIGKTLIRTVGLVAFWAL
                     FLCVYLGISAIASWTVSGAPSTLEIARSFALTLVPIAIGYHVAHYLVFLLVQGQYAIP
                     LISDPLGRGWDLFGTAGYRVDIGLIGARFAWYLALGAIVGGHVIAVYLAHIRAISIIS
                     RPRSALVTQVPMTAVMVLYTFIGLSIAAEPIVESRAGAEPIADASARIAIPSDAVLPA
                     SKSGLLNPVGPGQEAALKLTYRMLGSAFHDGTKTSPADLLYAYAFAYRWGTRSGDGDM
                     EYDPYIDAATAALRRDLKGVRITGSDTTSKSLRVGDVDFVREVFTVEVYLATSGETEI
                     SALIAPPWTTLPWTVLALMEEAVGRGWAAFSEAEATRRKIPQLDLVRSTALTSKLASL
                     LDQLEIQGFRPPALRALVTEEEARRRWESLSAFFKANGHFLVTNGPYKLRSWGPDHVT
                     LEAFRDLSYPLGVGSFDAFAVPRRGYITAADWNGRRLIVSGDIEVIERYQRSYRLIRT
                     PLKSVPRPTLLRSAPECRYFVTGPDGRVLAAGKVEASADAACLIDLGDRLPPGRYTLE
                     ALLAVNGNTMNADIKRFDLVIPPNQQGVD"
BASE COUNT         1488 a         2271 c         2250 g         1356 t
ORIGIN      
        1 cgctgacggt cggcttcttc gccaagttct atgccgtcgc cggcggagtg ggtgccgcgg
       61 cgtggcccgc aatcttcgcc cttgtcgcgg ggagtgtcat cggccttttc tattatctgc
      121 gcgtcatcgt catcatggcc acccccgtgg cagaggcaga agcggtcgaa ttcgaaaccg
      181 tcccttggac aggcggcgcg accttggcag cgctgacctt tatcctgata tggctaggcc
      241 tctatcctgc gccgctgatc tcgctcattc gcctgacggc gctgcaggtt ggaaactgaa
      301 gggatcgccg gacggagtgg accactgcgc agccgtgctc gcctgccatg gtcggcggcg
      361 agatcggggt gcatttttga cgttttttga cgttgatgaa tacggccccg agggccgcgc
      421 gcagaaatgc cgtcgcgcac caagcctgtt cctgcgtgcc cccattcccg atccgggcaa
      481 gtggttccgg cgcagcattt gcggcggcgt gccttgcgcg ttgactccgg ttatttgcgg
      541 agatgcaaat ttgcgccgga tcttcggcat tatttatcgc cgctggtcaa gcctgagtct
      601 cagggaagag atgcttcatt tcctcaggga gttgcccgcg cacttcctcg atctgaccag
      661 cactcaccgt ctcgccaatc gctttgaaga cagatttcat ggtgatggtc gcctgctctc
      721 ggtccaggcc aagagaacga gcaagctcat ccctgatcgc atcggcggtc acggcccgat
      781 caggcccatc cagacacttt tcgaggcgcg gctgcagcat cgaggggagc tgcgctataa
      841 gatcttcggc ttcctgcggg acgaggcgac gacagagcat gcaagcgccg agcagcagcg
      901 cccgttccgc gcggtcgcga tccagcccgg cggcatcagc aaccgtggtg actacccgat
      961 gataggtcgc ctcggcacgc gcctgcgccc gcatcagcgc gcgagcacgc ccggcggctt
     1021 gcagtttcgc gccagcctca ggatgcagcg tcccggccgg cttgtggggc gcctcgacct
     1081 cgagctgtgc tgaaacgatc gagcgcaagt cctcgatacc gaccgagccg tcgaccacaa
     1141 gatcatctag cgtgacgagg ccaaccaacc gctcgttctc ggtaatcgcg atgcgacgaa
     1201 cgccctcgtc ccgcatgagg cggaccacct cgccgatgtc cgcaccaatg tcgcaggaga
     1261 tcacgccctc ggacatcact tcgctgagcc gggttttttt cgggtcgagg tctccgccga
     1321 gcacagcaag agcaaggtcg cgatccgtga ccattcccac caagcctttt ggtccgccca
     1381 ccagcaccga gccgatgtga ttgtccgcca tggctcgagc tgcctgatag gccatcgcgc
     1441 tctgggggag gacgaccatt ctcgatctac gaaagcgctc aagagacatg ccacagcctc
     1501 cttattagga acttggatca gtcagcaagc catgaatgtg agtcacggaa tatcggcgaa
     1561 cccctgcgtc tgcgcagcat tggcctggat tgcgaccgca ctctcgttcg ttagatccat
     1621 cgaagcattg acggctgtca aaagcggatc cgaaccgatc aagagatcga tgcgaatacc
     1681 ctttaactgt tggctccctt tcgggatcct taaccgttgt cgccctttcg ggatccgctc
     1741 ggccgaattc cgggaatacg cgttgatgtt ttggcgccga gccaagctcc atctgacagg
     1801 gagaaagcac ctctggctta aatgatgaga ggcgctgcgg gtgccgcccc gggggaggcc
     1861 ccgacgctgt ttccgacgat ggcgccgagt ggacacctca tctgccacac gatccccgaa
     1921 ggagggtaag ccaaattcac ttcggcgccg agctgcgcct tgatggcatc tatgataacg
     1981 gctgagccaa agcctcgccg cgttggtgga gtgacagccg tgccggcggc ctcggtccag
     2041 ctcagctcga aaattgcggc tcccgtctcg tcgctctgga tttgccaatc caggcgaacg
     2101 gtgccggcgt cgtccttcaa tgcgccatgc ttgccggcgt tggtcgcaag ctcgtgcaac
     2161 accataccga ttgcttcggc ggccgtcggg gagagcatca gggtgggtcc gccccaccga
     2221 attcgattgc cgaacaggtt ggaaaagtgc gacagctgtg aacgcacgag atcgctcagc
     2281 tctacgcccc tccattcgtt tcggacaagc agatccaggc tcgtagaaag cgcttggatg
     2341 cggccctgga acaggtcgag tgcctgccga tgctcggccc tgaacgtctg ccgggcaatc
     2401 gccatcacca acgcgagcat attcctggac ctgtggttga cctcgcgcat cagcagctga
     2461 atgtgctcct catggcgctt ccgctcggtg atgtcgatgc aaacgccgac aattctgggt
     2521 gcgctgagct cgccgtccct gtgctgggat tgcaaacggc cgttccacgt catccagcgc
     2581 acttcgccgc ctgggcgcac gaacctgaaa tccatcatga ggtggccact gccgctgggc
     2641 gacagagcct cgttgtagag acggcggacg tgcgcgacat cctcgcggtg aacgcgggcg
     2701 aatgcagcct cgacatccgt ttccctcact tccgggaatc caagcagcct cgccagctcg
     2761 gaggaatagc ggacccgacc ggtttccacc tcgatctcga agatgccgat ttcggctgcc
     2821 tcgatcgcga gccgcaaccg ctgctcgctt tcgtgcagcg cccgctcagc gaggatgcgg
     2881 tcggtcgttt cggtcgttgc acaataaaag ccttccacat cgccggcatc gtttctcaat
     2941 ggcgtccagg aaaaagtgaa ccagctgacc ggccgctccg gtcgaccgct gatctccagt
     3001 ggacagtcca cgcaatgctg tccttgtccc gtcagcgtcg cctccaccag cggttggaga
     3061 tcatcccata tatctgccca gacctcccgc aatggccgtc caagagcgct cgggtgcttg
     3121 gcgccgagga tcggaatgta gccgtcattg tagagagaca cgagctctgg gccccaggcg
     3181 acatacgttg gttgagctgc gcccagcatg agttgaacgg ctgtcttgag cgggtgtccc
     3241 catgactcca ttggcccaag cgcagtcgag ccccaatcgt gcgcgcgaat gcgtgctgcc
     3301 atctcactgg tgagccacca agctcgaggt ttttcagtcg cggccatggt gtgcagacat
     3361 cgccctccga tcagtgtaag ccagtcccgc gccccccaac ctaatataag cggccgcatc
     3421 caggttagtt gcctccgggc aatcgcatac tcggtggcaa atgactgaat cggaatctga
     3481 ccacggctgc agcggagttg gtgacccgcg gacgatgtag ggtgagcttg ccgattcgca
     3541 ttgcgcgaag gtgctgcttg gcgtcggttc gtcctggcag aatcaacacc gggcaagccg
     3601 ttggcaatgg ggcatcaacc gatcggccat cagacagaga gagttccggc cgatggtttg
     3661 ccgccgattg acagctgaag cccgtcgtag gcgatgcgtg cagcaacgcc gtgctcacga
     3721 ccaagccgcc ggacgagcgt ttccatctct tgggatttgt cgcgtacgat ctgggagccg
     3781 caatgagtga agatggcctg ccgcacggca gcgtccttac accagccgag ttgcgctgcg
     3841 atcgaagcgt ggccgatcaa tgccccattc cgtcggcgca ccatggatcg gcggacggtc
     3901 gccccatcgc cgatgtagag gtctacgcct gaaagtgccc ctcgccgatc gaggatttca
     3961 gccacgtctg gtgcgtagaa aatgcagctc ccctcggcgc aaatccgcaa gccgacggcc
     4021 ggagcgagca acgaatgctc gactggaaat gcctggaaag tgagatggtc gatcaggagg
     4081 gggttccccg ggggaatcgt ccgtttcgcc tcgacgggaa atctgccgag cgcggcccac
     4141 gtctcgctcg tggcatacac cggacatggc gctcctgccg ccagtccacc ggcatgatcg
     4201 ggatgcgcat gggtgaggac gatggctgtc ggggcgaggg aattgagctg gtgcagccag
     4261 tcgctgccgc aatctatcat gacccgggta cgctcgctct caacgagaag cgagctatgc
     4321 cgaaggtgtc ttggcgaccg caggattata ccgccgcgcg ttccgagaaa cgtgagcttg
     4381 agcctggcca tttgctatcg atcaggaatg cccggtgtgc gccgaaaaca tcacgcgtcg
     4441 aatggcacat ccgttacctg cgactggaac ttcatccgaa gaaaccagac gttcgcccag
     4501 atgttcacgg cgcgagaaag cttcggccga aaccgggctt atgatctgaa agttcgatcg
     4561 ctggccagtc acggcggtct tacgcctctg ttcccagcgt aacccttgcg tcaatccaca
     4621 ccttgctggt tcggaggtat gaccagatca aagcgcttga tgtcggcgtt catcgtgttg
     4681 ccgtttacag ccagaagagc ttccaacgtg tagcgcccag gaggaaggcg atctcccagg
     4741 tcaattaggc acgctgcatc cgcactcgct tcgacctttc ccgccgcaag cactcggccg
     4801 tcaggtccgg tgacgaaata gcggcattcc ggtgcggatc ggagcagcgt cgggcgcggg
     4861 acagacttca ggggcgtgcg gatgaggcga tagctgcgct ggtatcgctc gatgacctcg
     4921 atgtcccccg agacgatcag gcgccggcca ttccaatctg cggccgtgat ataaccccga
     4981 cgcggaacgg cgaaagcatc aaaagagcca acgcccagcg gatagctcaa gtctcggaat
     5041 gcctccagcg tgacatggtc cggtccccaa ctccgcaatt tgtagggacc gttggtcacc
     5101 aggaagtgac cgttcgcctt gaagaaggca gaaagcgact cccaccgccg gcgcgcctcc
     5161 tcttccgtca caagggctcg gagggccggc ggtcgaaagc cttggatttc gagctggtcg
     5221 aggagtgatg ccagtttgga ggtcagcgcc gtcgagcgca ccagatcaag ctgcggaatt
     5281 ttgcgccgcg tggcctcggc ttcggaaaac gcagcccagc cgcggccgac cgcctcttcc
     5341 atcagagcga gcaccgtcca cggcagcgtc gtccagggcg gagcgatcaa tgcgctgatc
     5401 tccgtctccc cagatgtcgc cagatagact tcgacggtga aaacttcgcg cacgaaatcg
     5461 acgtcaccaa cgcgcagcga tttcgaggta gtgtcgctgc cggtgatgcg tacgcctttc
     5521 agatcgcggc gcaacgcggc cgtcgcggcg tcaatatatg ggtcgtattc catgtctccg
     5581 tcgcctgacc gggtgcccca gcgataggcg aaggcatagg cgtagagcag gtccgcggga
     5641 ctggtctttg ttccgtcgtg aaaggcagag cccagcatcc ggtaggtgag cttcaatgcg
     5701 gcctcctgac ctggtcccac ggggttcagc aggccgcttt tgctggcagg cagaacggcg
     5761 tccgacggga ttgcgattct tgcgctcgca tccgcgatcg gttcagcgcc ggctcgactc
     5821 tcgacaatgg gctctgcggc aattgaaagg ccgatgaacg tatagagcac catcactgca
     5881 gtcatcggca cttgcgtcac cagggcagag cgaggtcggg agataattga aattgctctg
     5941 atatgcgcaa gatagacggc gatcacgtgt ccgccgacga tcgcgccaag agcgagatac
     6001 caggcgaaac gtgctccgat cagcccgata tcgacccgat accctgcagt tccgaacagg
     6061 tcccagccgc gcccgagtgg atcggaaatg agaggaatgg cgtactgtcc ctggaccagc
     6121 aggaatacga ggtaatgggc gacgtggtat ccgatcgcaa ttggcacaag tgtcaaagcg
     6181 aagctccggg cgatttccaa agtggaagga gcgccggaaa ccgtccaact ggcgatggct
     6241 gaaatgccga gatagacgca aaggaagaga gcccaaaaag ccacaaggcc gaccgtcctg
     6301 atcaaggttt ttccaatgtc atgagcatgt gcctggagcg cgctctcgaa cagagcccat
     6361 tcgccggtgc cgataaggcc gtcgtaaagc acggtggcaa gcagcagcag ggtgaacgcg
     6421 accatggacg tcgagacggt cggactttca accagacccg acccgaatgg gcgcaccaga
     6481 acgcgcccct tcttgacctc aagcggtgaa aagcgggcga atgtgccaaa gaagagggag
     6541 aatacctcac agtgcttcag ccatatatct cggccgaaca gcgccatccc ggcccaggtc
     6601 aggatggagt aggcgatagc gagcttggcg atgtgcgcag gcgaggcggc gttcggatcg
     6661 atgagctcga cccaggcgaa tgcgagcaga aaaatcgacg ccggccatac gcccagagcc
     6721 ccaggatagc gaagcacagg ctcggcggcg ccctcccacc ctaggcgtcg gagggcgaga
     6781 tggacaccgt cgaaagccgt tgtccaggga ttgacgagcg cccagacgtt gccaatgaac
     6841 gccgagagga atgcgaaacc gacccaccag atgatccaca ccagcgtcgg gacgatgttt
     6901 cgataagggt tctgatctcc cagaaagccc gcaacaatca ccacgagaaa caacgcgagg
     6961 gctgcgagcc gcatacccaa aacgacagtc cagtgggaca gcagccggcc tacggggtgg
     7021 gtgaggagat ttttcgggga gcgctcccgc ggcgcaggcg cgcggtgtac aaacagtccg
     7081 aaagccagga aagagagcgc cactacggcc gccgcgccgt aaacgtatag ctccagcggc
     7141 aacggcagct cgtaacgctc gccgaagccg tgggcggctg ccggcgttgc tgcgacggca
     7201 gccagcgtgg cactcgttac gagctgcgta ggtcgaacgg cgacattatc gtggccagac
     7261 ctcgacacgg acaatggggg cctccccatg agaatgaccg gcgcgggaat cgggggaatg
     7321 ctcctccacc gggaaccgcc ctgtcgcttg cgccgtaaag ctcat
//
LOCUS       DASVDU010000241        24886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_90987, whole genome shotgun sequence.
ACCESSION   DASVDU010000241 DASVDU010000000
VERSION     DASVDU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 24886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24886
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90987"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..912
                     /locus_tag="VF226_15510"
     CDS             406..912
                     /locus_tag="VF226_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEX7075446.1"
                     /translation="MPATRADLFARLDQLGIKTRTVEHEPVFTVAESEKLERDLPGGH
                     TKNLFLKDAKGKLFLLTAESHSPINLKILHKILGCARLSFGKPELLMEVLGVAPGSVT
                     AFALINDRDRRVSFLLDERLMAYETINCHPLENTATTNIARDDLLRFIRSCGHEPRIV
                     GLVDAPIV"
     gene            988..1896
                     /gene="trxA"
                     /locus_tag="VF226_15515"
     CDS             988..1896
                     /gene="trxA"
                     /locus_tag="VF226_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7075447.1"
                     /translation="MEIGARQPAAAGQSDVIKDTTTANFAKDVLEASRSALVLVDFWA
                     AWCGPCRQLTPILEKVVRSYNGKVRLVKLNVDEHPAIAGQLRVQSLPTVYAFRDGRPL
                     DGFMGAQPESAVKAFIDRLVGEESEADVAAALETAEQALEAGDLQQAAEIYAAVLQED
                     RQNVAALAGLAKCYLKSGDTARAEQTIALVPPDKKQTSAVASVVAALELAKKAGAGSN
                     TAELQAKVAANPADHQARFDLALALAANGDKAGALEHLLELFRRDRNWNEEAARKQLI
                     QLFDAWGPKDPFTIEGRRKLSSLMFS"
     gene            1922..2605
                     /locus_tag="VF226_15520"
     CDS             1922..2605
                     /locus_tag="VF226_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7075448.1"
                     /translation="MAPTDRYRRPADLPQRIPVFPLRGVILLPRAVLPLSIYETRYLA
                     MLEDIVAGDRIVGIVQPERSESDEESPQGKDVSLRTIGCAGRLTAFQELDDDRVLVTV
                     TGVARFGLVSERPTEKPYRIFEVDYDRFSDDFREGVGEDRVDRSTLLRVLRDYLEAHK
                     LRADWRAILSAPSEYLVNSLSVVCPYGPEEKQALLEAEDLKTRAEVLVALAEMELASS
                     ESGSGGAMN"
     gene            2629..2856
                     /locus_tag="VF226_15525"
     CDS             2629..2856
                     /locus_tag="VF226_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX7075449.1"
                     /translation="MTDETPPSPNPSQNDTVDRRLLELLVCPLTKTRLEYDRDRQELI
                     SRAAGLAFPIREGVPLMTVDAARPLDEEQAK"
     gene            complement(2875..6399)
                     /locus_tag="VF226_15530"
     CDS             complement(2875..6399)
                     /locus_tag="VF226_15530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HEX7075450.1"
                     /translation="MFDGWAILALGIAYICVLFAIAWTGDRTIRSRKGGQGRPLIYAL
                     SLGVYCTSWTFFGSVGLAASTGYDFIPVYVGPILMFAFGWPLILRIVRLSKSQNITSI
                     ADFLAARYGKSQAVAAVVTAVAVVGTLPYIALQLKAVVISTEALLVGNLHLPEEWAGV
                     AGFGETALLAAIALAAFAVLFGTRHIDATEHQDGLMLAVAAESLVKLTAFLTLGLFVT
                     VSVFGGIGGFAETLRQSDEVQATFVRPFNGGVWLTVSFLSFVCIILLPRQFHVTVVEN
                     NSEREIQRARWMFPVYLVLINIFVVPIAAAGLLIFPDRSIDADLFVLSLPISREANLI
                     TTVAYIGGLSAATAMVIVESIALAIMVCNGLVIPLLLRQRLLEASEQDNLSSVLLVIR
                     RAAIVLIVLLGYLVYRALGEAQGLAAIGLISFAAIAQLAPAFFGGLFWRKGTARGAIA
                     GILAGFAVWAYTLLLPWVVKAGWLGMDILQHGPLGITMLRPQALFFLEFEPLTHGVLW
                     SIFANVTALVMVSLLRPPEPVERLQAHVFVQDDLPRMTPTPSFRLWRTSITVGDLQAT
                     VARYLGSERAERSFAEFGESRNMKLSPHAEADIHIIRFTEHLLTSAIGAASSRLVLSL
                     LLRRSNVGSQSALRLLDDASEALQYNRDLLQSALDQVRHGLSVFDKDLKLICWNRQFR
                     ELLDLPPDLGRVGMPLDRILRACAERGDFGEGPIDELVADRLVRLAVRKETVQEKLSN
                     GRILEIRTAAMPQGGVVATYSDITDRVAAASALARANETLERRVRERTAELLRVNAAL
                     AVAKAKADEANLDKTRFLAAASHDLLQPLNAARLYASSLVERRLSEDDAVLARNIDAS
                     LAAVEDILGALIDISRMDAGRLEPDITDVPLAELFSYLEVEFTPSAREKGLELRILQT
                     SLSVRTDRKLLRRVLQNLVANAIKYTSKGGVLVGARRRNGGVLIQVIDTGRGIPASKH
                     ELIFKEFQRLEDSGSEIRGLGLGLSIVERICRVLQVPIKLNSRPGHGSTFSVLLPAAT
                     RSPAKVPTVKAPIQGPIAGCTVLCIDNEPAVLDGMETLLVGWGCRVLTASSGSSALEA
                     IEQANVVPDIILADYHLDEQTGLEAVIGLREALKADMPVVFITADHSPEVERELRARG
                     FMLLRKPLKAASLRALMNRLTLRRVAAE"
     gene            6673..7065
                     /gene="mscL"
                     /locus_tag="VF226_15535"
     CDS             6673..7065
                     /gene="mscL"
                     /locus_tag="VF226_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX7075451.1"
                     /translation="MGIIDEFKQFAMRGNVLDLAIAVIMGAAFGPVVTTLVDGIIMPP
                     IGYILAGIDFSQLSLTLPTPTGDGVEIKYGLFLNAIITFLITAFAVFLLVKAVNRFRK
                     PAPAAPKVEPPTEAYLREIRDALVKRQP"
     gene            complement(7070..8059)
                     /locus_tag="VF226_15540"
     CDS             complement(7070..8059)
                     /locus_tag="VF226_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7075452.1"
                     /translation="MTLVEKPFARRPRPIGKFEDPDWTATGERRAVVPLTGLETLWIN
                     TGTLCNIQCRNCYIESSPRNDRLEYISAAEVAAYLDEIAELKLPTREIGFTGGEPFLN
                     PEIVPMVGDALARGLRVLVLTNAMRPMQRPKVKAGLLALLAAHGSRLKIRVSLDHYTK
                     ARHESERGPRTWSKTLEGLDWLAANRFNIAIAGRTCWGEDEPAARAGYQRLIAERGWP
                     IDAQDPSQLTLLPEMDGHREVPEITTACWGILGKSPGDVMCASSRMIVKRKGAASPVV
                     VPCTLLPYEPAFEMGRTLAEAAKADGGMFSQGAVKLCHPHCAKFCVLGGGSCS"
     gene            complement(8208..8432)
                     /locus_tag="VF226_15545"
     CDS             complement(8208..8432)
                     /locus_tag="VF226_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075453.1"
                     /translation="MPVTFEHIEELKKRSEENKKAQPDQRTHLALELIADALILTLEQ
                     ELDEDLADEVEEEEDEGDWEETEESAASDE"
     gene            8629..8703
                     /locus_tag="VF226_15550"
     tRNA            8629..8703
                     /locus_tag="VF226_15550"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8661..8663,aa:Gly,seq:ccc)
     gene            complement(8713..10206)
                     /gene="glpK"
                     /locus_tag="VF226_15555"
     CDS             complement(8713..10206)
                     /gene="glpK"
                     /locus_tag="VF226_15555"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX7075454.1"
                     /translation="MSGFILAIDQGTTSTRAIVFDRDYRIRGLGQQEFTQHFPRSGWV
                     EHDPQDLWRTTVETVRTALAKSGVPASEIAGCGITNQRETTLLWDRETGVPVGPAIVW
                     QDRRTSDVCAELRADGLEQEITERTGLLLDPYFSATKLAWLLDTIEGARSRAEQGRLA
                     FGTVDSWLLWNLTGGKVHATEVTNASRTLLFNIRQQAWDPELLRIFRIPSAILPEVRD
                     TAAEFGTTDPDLFGAPIPIRGIAGDQQAATVGQACFTPGMMKSTYGTGCFAVLNTGAD
                     LVPSRNRLLTTIAYRLDGRTTYALEGSIFVAGAAVQWLRDGLKILERADQSAQMAAAA
                     DPNQDVYLVPAFTGLGAPHWNADARGALYGLTRNTGPNELARAALEAVCYQTCDLVEA
                     MRRDWSDGAGTVLRVDGGMVASDLTMQWLADLLDAPVDRPAVLETTSLGAAWLAGRQA
                     GVWPGEEGFAESWRCERRFEPSMDAAIRKRKLAGWRDAVRRTLSLGA"
     gene            complement(10279..12093)
                     /locus_tag="VF226_15560"
     CDS             complement(10279..12093)
                     /locus_tag="VF226_15560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075455.1"
                     /translation="MNYHNQRSFDWEGRQQDLVAAEAEYGFLIGRLLDRETLRQAILT
                     AESWGVSPLEVLLARGWVTDVAYVGALADYLGVPALGQFGAELPDEAILIDATSRSPG
                     QVARQIAERESAGGIAVIATAREMEERDSSEREQWRLKRAIAGLLRWRPVLSAGAPTW
                     TWQLITASALSGLVAGVTTVAPDAGYRLLLVLLTLVFLPVVVLRVVVTALGLMPQRSS
                     DASTHLADADLPTYSILVPMFREAQVLPDLVAAISALDYPAAKLDVLLILESVDLETQ
                     DIARSIELPGFMRTIVVPDSQPRTKPKALNYALQFARGDYVVVYDAEDMPEPDQLRLA
                     IATFREAPRGTICLQGQLNIHNARESWLTRQFALEYSALFDVTLPGLARLGLPIPLGG
                     TSNHFPREVLDRWFGWDPFNVTEDADLGIRLARTGGRIGVLNSTTWEEAPARFGIWLR
                     QRTRWMKGWMQTYLVHTRQPVRLMRELGFAAFVGFHLYSGGLILSALVFPLFCGMAAF
                     EAWQGGWLMLPESLSGGALWTLTVFELAAAYASSVLAAGVAAWRRGRKWLAFRAPIMP
                     VYWLLISVAAYRALFQLATAPHFWEKTEHRSRTRSRRP"
     gene            12252..13073
                     /locus_tag="VF226_15565"
     CDS             12252..13073
                     /locus_tag="VF226_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7075456.1"
                     /translation="MKRPFQFLAAVLSVLLLAGVNQVVAQEPDPDNPASDQGARRLVL
                     RFVTDGDFPPFNYFDEDGILTGFNIDLARALCLEANTSCDIQARPWDTLIPALERGDA
                     DAVIAGHRITPQLLQTVDFTERYFRTPARFAARRDSEKIEISPEELDGKSIAVAKGTA
                     HSAYLKAFFRFSKIREYDSPEQAREAVMNGKADFVFDDGIGLSFWVVGTISHECCELR
                     GGAYYEPKYFGDGLAIAVSKRDPNLKHLLNDALKRVRASGRYEELVLRYFPNRVY"
     gene            13186..14166
                     /locus_tag="VF226_15570"
     CDS             13186..14166
                     /locus_tag="VF226_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7075457.1"
                     /translation="MSSRLLPGREELVICFAHVAYRMGEAFARRNTGISWLEVRSAEE
                     LQEAAAKAHVIVTSGFWRNELLDAASNLRFVQSISAGTDQYDKERFREKGVRLASAKG
                     VNANAVAEHAMALILALSRQLHTGRDHQKERHWRGMIGDPSLREDELGGKTLLIVGLG
                     SIGARLARLAKAFDLNVIATKRDPSSGGDVADSVHPQSQLKDLLPRADIVALTCPLTP
                     ETENLIDAKALSLMKTSAHLINVARGRVVDEDALITALQTGQIAAAGIDVTREEPLPP
                     SSPLWAMPNVLLTPHTAGETRRYEDNVLDIMIENIERLWRGEATLRNEVV"
     gene            14441..15667
                     /locus_tag="VF226_15575"
     CDS             14441..15667
                     /locus_tag="VF226_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7075458.1"
                     /translation="MEVSTDDREPLRPAEDLAAPDNIGRGSPEAVAIAPVERPSTMVP
                     PWAGSKPGFALRPSRESHPRDSRVYAALDLGTNNCRLLVARPSRRGFQVVDAFSRIIR
                     LGEGVGTTGCLSEAAMSRTIDALKVCAAKMRRHNVVRSRLVATEACRVAKNGPEFISR
                     VSRQLGLEIDILSREDEARLAVSGCATLLDDRSDLALVFDIGGGSSELVWLDLTRRRR
                     SWRRSLGDRLDAMNCIAAWTSLPVGVVTLAERYGGRQLTAAGFEAMVDTVLELLAPFE
                     AKHCFKAQVERGRAHLLGTSGTVTTMAGIRLGLPRYDRNRVDGCWLDAAEARAITYDL
                     LARSHEQRVAEPCIGKDRADLVLAGCAILEGLLRMWPAKRLRVADRGLREGMLATMMA
                     EDGCYRAFDRRARRPN"
     gene            15707..16432
                     /locus_tag="VF226_15580"
     CDS             15707..16432
                     /locus_tag="VF226_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX7075459.1"
                     /translation="MVKSPKQGSGLGGQRQLKVRLKSGKRRTASSRRWLERQLRDPYV
                     AAAQREGYRSRAAYKLIEIDDKYGFLKPGQRVVDLGAAPGGWSQVAAARVKSVEGHGQ
                     VVAIDISEMEPLAGVEVLHLDFMDPSAPDALKARLRDGGADVVLSDMAAPTVGHAKTD
                     HLRIMALAEAAAEFAREVLSPGGVFLAKVFQGGTEGELLASLKRDFAKVRHVKPPASR
                     SDSAELYVLAIGFRGNGHSTGAR"
     gene            16669..18165
                     /gene="guaB"
                     /locus_tag="VF226_15585"
     CDS             16669..18165
                     /gene="guaB"
                     /locus_tag="VF226_15585"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7075460.1"
                     /translation="MAARPIEIVENVGLTFDDVLLVPQSSETLPGQVDVSTRITKTVT
                     LNIPILSSAMDTVTESRLAIAMAQAGGIGVIHRNLTPEQQAKEVEAVKKFESGMVVNP
                     LTIHPNATLKDALDLMKEYRISGIPVVESDRSGRGGKLVGILTHRDVRFATNPETPVS
                     ELMTKDRLVTVKAGVGREEAKRLLHQHRIEKLLVVDDEYRCVGLITVKDIEKATLFPN
                     ACKDEQGRLRVAAATTVGDDGHERTERLIAAGCDVVVVDTAHGHSRRVLDAVTRIKRL
                     SNSTQVIAGNVATAEATRALIDAGADAVKVGIGPGSICTTRVVAGVGVPQLTAILDCV
                     AEARKSGTPVIADGGVKFSGDITKALAAGADAVMIGSLLAGTDESPGETYLYQGRTYK
                     AYRGMGSVGAMARGSADRYFQQEVKDTLKLVPEGIEGQVPYKGPVSTVLHQLVGGLRA
                     GMGYVGARSLAELQARAKFVRISPAAIRESHTHGVGITRESPNYPGNG"
     gene            18313..19590
                     /locus_tag="VF226_15590"
     CDS             18313..19590
                     /locus_tag="VF226_15590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX7075461.1"
                     /translation="MKAAVEVLDEIFVRHRPAAQALADWGKAHRFAGSGDRAAIGNLV
                     YDVLRRRLSLAARMSDESARALVLAAASDALGLTPDEIAAAADGSEYALAPLSEAERA
                     GLVRDVPSDAPMHVQADIPEWLAPSFERLFADRAVEEGRALAQRAPVDLRVNTLKADR
                     ARVLKALARYGAEPTPLSPVGIRIPPPTGPGRTPNVEAEPGHAKGWFEIQDEASQIAA
                     LLAGASPRLQVLDLCAGAGGKTLAMAAAMQNTGQVYAYDADRMQLRPIFERLRRAGVR
                     NVQVLPAGDEGAVKALGPRFDVVFVDAPCTGSGVWRRRPDSKWRVKPQNLADRQKEQQ
                     EVLELGAPLVKPGGRLVYVTCSILPEENRDQVSDFLARHSDFSLRPYAHAWAETIGGA
                     PPRSADGGTDALLLTPTRHGTDGFFIAILVRNK"
     gene            19799..21799
                     /locus_tag="VF226_15595"
     CDS             19799..21799
                     /locus_tag="VF226_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7075462.1"
                     /translation="MAIASKVKRPRRASARSCLGPVADLEAHLPQEWWRKLFNALYVK
                     TDGDVVENIENTRREVDFIVSAAAIHPHSAILDLCCGQGRHCLELARRGFRNVTGVDR
                     SRYLIRLARKRAQGEGLQVVFKEGDARNPRLPESSFDCVAIMGNSFGYFSNKQDDEKV
                     LTTVGKILRPTGQLVLDITDGAYMAEHFERRSWEWIDEHHFVCRERSISQDRERLISR
                     EVIVHDEMGVIADQFYAERLYTKESIAKLLAKTGFRNVRYHGHAEALSDRDQDLGMMA
                     RRILLTADAPQRPARKSRGKVQKLDVTVLLGDPRLPDDVKRGGTFNPEDLETIRKLKD
                     ALSTLPNYTFRYLDNHATLERDLAGLRTDLVFNLCDEGLNNDPFKELHVPAMLEVLGL
                     PYTGAAPQALAACYDKGLVRAVAMTLDVPVPLETYVRPGDQGATLPSVFPALLKPNYG
                     DSSQGITKDAVVTNERALLDYLERLRAEFPRRPILVQEYLTGPEYSVSLIGNPDQGLR
                     ALPILEVDYSRLDPSLPKILGYESKWEPDSPYWTQIRYHEANLPDHIQAQLIEHSARL
                     FERLGCRDYARFDFRADAHGEIKLLEVNPNPGWCWDGKLNIMAGFQGMRYAELLGQIL
                     AAAEERLGIVPKPHALTVGAAPNGVHGGAQAHSNAVMAPQMK"
     gene            complement(22003..23628)
                     /locus_tag="VF226_15600"
     CDS             complement(22003..23628)
                     /locus_tag="VF226_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7075463.1"
                     /translation="MVEERPELRKRPANFVPLTPVSFLARAANFFANQTAVIHGARRF
                     TYADLYARSRRLAHALTKAGVGRGETVAILAPNTPALLEAHYAVPMIGAVLNPINIRL
                     DPATIAFCLQHGEAKVLLADREFASSVLPALERLETKPLVIDIADWEAPEAPAFGSVE
                     YEDFLATGDSAFAYPGPEDEWDSICLLYTSGTTGNPKGAVYSHRGAHLNALANALTFK
                     LDHNSRYLWTLPMFHCSGWTYTWAVTAAVGTHICLRKIEPKRIFDLIAEHEVTHMCGA
                     PIVLNTLIHAPAETKRPLPTRPKVMTGGAAPPSAVIRNMEAMGFEVLHAYGTTESYGP
                     STTCVHMRDWDNGPEEQRFTNMARQGIPHVLIEDMTVADPVTLGPVARDCQAIGEIML
                     RGNTLMKGYLKNPRATDEAFAGDLYHSGDLAVWHPDGYIEVKDRSKDIIISGGENISS
                     LELEEVLYRHPQIMEAAVVAKPDPKWGETPCAFVTLKPDAEPTTAEDIIAYCRANVAG
                     FKVPKTVVFGPLPKTSTGKIQKFVLRERAREIA"
     gene            complement(23707..24066)
                     /locus_tag="VF226_15605"
     CDS             complement(23707..24066)
                     /locus_tag="VF226_15605"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEX7075464.1"
                     /translation="MAVKRISAETLIELAVETLRGEIQPSLPPEHRYTAAMIANALEI
                     ARREILTDGETARWELLDELYPEGDGDMKQLALDIRSGKVSTNTVPDLHQRLRAILVE
                     ELRIRNPRFLRSRQGRE"
     gene            complement(24057..>24886)
                     /locus_tag="VF226_15610"
     CDS             complement(24057..>24886)
                     /locus_tag="VF226_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX7075465.1"
                     /translation="RAGEFGVLKAAHAAGVAVAEPIARCADADVIGAPFLIQAFVPGV
                     AQARRIVRDPALSDFGESLAESLGAELAKIHSITPPRDDLSFLPIPMLAPARNEVARL
                     RNALSPATEPRPALEYVLAWLDAHAPKPEPITLVHGDFRTGNYIVSEGRLGAVLDWEF
                     AHWGDRHEDIGWIMARCWRFGSDALETGGIASREAFYRGYRSAAALPFDESLIPYWEI
                     MAAAKWASVAVLQGDRYRKGGENSIELALTGLMPSEMELDALDGIAALMGSGDPRWR"
BASE COUNT         4355 a         8063 c         8230 g         4238 t
ORIGIN      
        1 gacaaagtcc gccacgaatg ggcgctgatc tgcaccgccc acaacctcac caagctcgcc
       61 agggccgcat gacaggccta tccaacctca ccaattgcct cgcccaacat atctggacag
      121 gctcctagcc gacagtcccc gagttcctcg gctagatcaa tgcccgaaat gccgatctaa
      181 gatggccaga caggacgttc gcgaaaactg gcgctgacgg gcagggtatc agctctgtgg
      241 ggagggcatc tgaaagacgc ctgagcctga tggcgttacc actcccacga tcgcgatctg
      301 gccgccccag gtttggcccc tgcccttggt tgcgtcactg gccgcaaggg gttagatcct
      361 gcccagccag ccccaatccg aacacctgcc cgaggccgcc ccgagatgcc tgcaacacgc
      421 gccgatctgt ttgcccgtct cgaccagctc ggcatcaaga cccggaccgt cgagcacgag
      481 cccgtcttca ccgttgccga aagcgagaag ctggagcgcg acctgcccgg agggcacacg
      541 aagaacctgt tcctgaagga tgccaagggc aagctgttcc tcttgacggc agagagtcac
      601 agccccatca acctcaaaat attgcacaag attttgggct gcgcgcgatt gagcttcggg
      661 aagccggagc tgctgatgga ggtgcttggc gtggcgcccg gctcggtcac ggcgttcgcg
      721 ctcatcaacg accgggaccg ccgggtcagc ttcctgctcg acgagcggtt gatggcttat
      781 gagacgataa actgccatcc gctcgagaac accgccacga cgaacattgc gcgtgacgac
      841 cttttgcggt tcattcgctc ctgtggacat gagccgcgaa ttgttggact ggtcgacgcg
      901 cccattgtct gaggcgcgac ctgccacgat cttgaggttt tccgcttgag tgcaccgcgg
      961 aagacggagc cggccgagga gaaccagatg gaaattggag cacgccagcc tgctgcggca
     1021 ggtcagtcgg atgtgatcaa ggatacgaca accgcgaact tcgccaagga cgtgctggaa
     1081 gcgtcgcggt ctgcactggt cctggtggat ttctgggctg cgtggtgcgg cccctgcagg
     1141 cagctcacgc cgattctcga gaaggtcgtg aggagctaca acggcaaggt gcggctggtg
     1201 aagctcaacg tggatgagca cccggccatt gccggacagc ttcgcgtgca gtcgctaccc
     1261 acggtctacg ccttccgcga tggtcgtccg ctcgacggct tcatgggcgc ccagcccgag
     1321 agcgccgtga aggctttcat cgaccgtctg gtcggcgagg agagtgaagc cgatgtcgct
     1381 gccgcgctgg agacggccga gcaggcgctg gaagccggcg acctccagca ggcggccgag
     1441 atttacgccg ccgtccttca agaggaccgt cagaacgtag ccgcgctggc ggggctcgcg
     1501 aaatgctatc tgaagagcgg ggatacggct cgcgcggagc agaccatcgc tctggttccg
     1561 ccggacaaga agcagacgag cgctgtcgcc agcgtggtcg ccgccctgga gctcgccaag
     1621 aaggcaggcg ccggttcgaa tacggccgag ctgcaggcga aggtcgccgc caacccggcc
     1681 gatcatcagg cgcgcttcga ccttgcgctg gctctggccg caaacggcga caaggccggt
     1741 gcgctggagc atctccttga gctcttccgc cgcgaccgga actggaatga ggaggccgcg
     1801 cgcaagcagc tcatccagct gttcgacgcc tgggggccga aggacccgtt caccatcgag
     1861 ggccggcgca agttgtcgtc gctgatgttc tcctgaggcc cgaagctcga gggaggccgg
     1921 cttggcccct acggaccgtt atcgtcgtcc ggccgatctg ccgcagcgga tccccgtttt
     1981 tccgctgcgc ggggtaatcc tgctgccgcg ggccgtgctg cccctgagca tctacgagac
     2041 ccgctatctc gcgatgctcg aggacatcgt cgcgggcgat cgaatcgtgg gcatcgtgca
     2101 gcccgaacgg tctgagagcg atgaggagag tccgcagggc aaggacgtga gcctgcggac
     2161 gatcggctgt gccggccgcc tcaccgcgtt ccaggagctg gatgatgacc gggtgctcgt
     2221 aacggtcacc ggcgtcgcgc ggttcggcct cgtctcagag cggccgaccg agaagcccta
     2281 tcggattttc gaggtcgact acgaccgctt cagcgacgac ttccgggagg gtgtgggtga
     2341 ggaccgcgtg gacaggtcta cactgctgcg cgttctcagg gattatctcg aggcgcacaa
     2401 gctgagggcc gactggcgcg ccatcctgag cgcaccgagc gagtatctgg tcaactcgct
     2461 gtccgtcgtc tgcccctatg gtcccgagga gaagcaggcc ctcctcgaag cggaggacct
     2521 gaagacccgc gccgaggttc tggtggcgct cgccgagatg gagctcgcct ccagtgagag
     2581 cggatcgggc ggggcgatga attgatgcca agccgatggg cacgcgccat gacggacgaa
     2641 acaccgccat cacccaaccc gagccagaac gatacggtcg accgccgctt gctggagctg
     2701 ctggtctgcc ctctgaccaa gacgcgactg gagtacgacc gcgaccgcca ggagctcatc
     2761 agccgcgccg ccggtctcgc ttttccaatt cgggagggcg ttcccctgat gaccgtcgat
     2821 gcggcccggc ccctcgatga agaacaggca aagtagccga tccgcgagat cacctcattc
     2881 ggccgcgacg cgccgcagtg tgagccggtt catcaacgcg cggagggaag ccgctttcag
     2941 cggctttcga agcagcatga agccacgggc gcgcaactcg cgttcgacct ccggcgaatg
     3001 atcggcagtg atgaacacga ccggcatgtc ggccttgagc gcttctcgca gcccgatgac
     3061 cgcctcgagg cccgtttgct cgtcgaggtg gtagtcggcg aggatgatgt cgggaacgac
     3121 gttcgcctgt tcgatggcct ccagtgcgct actgccgctg ctcgcggtga gcaccctgca
     3181 tccccagccg acaagaagcg tctccatccc atcgagaacg gcgggctcat tgtcgatgca
     3241 aagcacggtg caaccggcga tcgggccctg aatgggggct ttcaccgtcg gtacctttgc
     3301 aggcgaacgg gtggcggcgg gcagcaatac agagaaggtc gagccgtggc ccggtctcga
     3361 gttcaacttg atgggcacct gcagcacgcg gcagatccgc tcgacgatcg agaggccaag
     3421 ccccagcccg cgaatttcgg acccggaatc ctcgaggcgc tggaattcct tgaagatcag
     3481 ctcgtgcttg gaggccggaa tgccgcgccc tgtatcgatc acctggatca gcaccccgcc
     3541 gttccgccgg cgcgcaccga ccagcacgcc gcccttgctg gtgtatttga tggcgttcgc
     3601 cacgaggttc tgaagcaccc ggcgcagcag cttgcgatcg gtgcgcaccg agagtgacgt
     3661 ctgcaggatc ctgagctcca ggcccttctc ccgggccgag ggtgtgaatt ccacctccag
     3721 gtaggagaaa agctccgcca gcggcacatc ggtgatgtcc ggctcgaggc ggcccgcgtc
     3781 catgcgcgag atgtcgatga gcgcgccgag gatgtcctcg accgccgcca gcgaggcatc
     3841 gatgttgcgg gcgaggacgg catcatcctc gctcaaacgc cgctccacca ggctcgaggc
     3901 atagagccgc gccgcgttga gcggctgcag aaggtcgtgg ctcgccgcgg ccaggaagcg
     3961 ggtcttgtcg agattggcct cgtccgcttt ggccttggcc acggcaagcg ccgcattgac
     4021 ccgcagcagt tccgccgtgc gctcgcggac acgccgctcg agcgtctcgt tggccctggc
     4081 cagagcgctt gccgcggcca cccggtccgt aatgtcggag tacgtcgcga ccacccctcc
     4141 ttgcggcatg gcggcggtgc ggatctccaa gatccggccg ttactcagct tctcctggac
     4201 ggtttctttc cggacggcga ggcgcaccag cctgtccgcg accagctcgt cgatcggccc
     4261 ctccccgaag tcgccccgct ccgcgcaggc gcgcaggatg cggtcgagag gcatgccgac
     4321 ccggccaagg tccgggggca ggtcgagcag ctccctgaac tgccggttcc agcagatgag
     4381 cttcaggtcc ttgtcgaaga cgctgagtcc atgccgcacc tgatcgagcg ccgattgcaa
     4441 caggtcgcga ttgtactgca acgcctcgga ggcatcgtcg aggagacgca gggccgactg
     4501 gctgccgacg ttgctccgcc tcagcagcag cgacaggact agccgcgagg aggcggcccc
     4561 gatggcgctc gtcagcagat gctcggtgaa gcgaatgatg tggatgtcgg cttcggcgtg
     4621 cggggagagc ttcatgttgc ggctctcgcc gaactccgcg aacgagcgct cggcccgctc
     4681 ggacccgaga tagcgcgcta ccgtggcctg cagatcaccg acggtgatgg atgtgcgcca
     4741 tagccgaaag ctcggcgtgg gcgtcatgcg cggcaggtcg tcctgcacga agacatgcgc
     4801 ctgtaaccgc tcgaccggct cgggcggacg cagcagcgag accatcacca gcgccgtcac
     4861 gttggcgaag atgctccaca ggacgccgtg ggtcaggggc tcgaactcga ggaaaaagag
     4921 cgcttgcggc cgtagcatcg tgatgccgag cggcccgtgc tgcaggatgt ccatcccgag
     4981 ccagccggct ttgacgaccc aaggcagcag cagcgtgtac gcccagacgg cgaagccggc
     5041 taggatgccg gcaatggcgc cgcgcgccgt ccccttccgc cagaaaagcc cgccgaagaa
     5101 tgcgggcgcg agttgcgcga tggcggcgaa ggagatgaga ccgatggccg ccaggccctg
     5161 ggcctcgccg agggcgcggt aaacgagata gccgagcagg acgatcaaca cgatcgcagc
     5221 gcggcgaatg accagcagga cgctcgacag gttgtcctgc tcgctcgctt cgagcagccg
     5281 ctgtcttagc aggagcggaa tgacgaggcc gttgcagacc atgatcgcga gcgcgatcga
     5341 ctccacgatc accatcgccg tcgctgccga caggcctcct atgtaggcga ccgtcgtgat
     5401 caggttggcc tcgcgcgaga tcggcagcga cagcacgaag aggtctgcat cgatggagcg
     5461 atcgggaaag atcagcaggc cggccgccgc aatcggcacc acgaagatgt tgatgaggac
     5521 caggtagacg gggaacatcc agcgcgcccg ctggatctcg cgctcggagt tgttctcgac
     5581 gacggtcaca tggaactggc gcggcagcag gatgatgcag acaaagctca ggaacgagac
     5641 ggtgagccag acgccgccgt tgaatggcct gacgaacgtc gcctgcacct cgtcgctttg
     5701 gcgcagggtc tccgcgaacc ctccgattcc gccgaacacc gaaaccgtga cgaagaggcc
     5761 aagggtgagg aacgccgtaa gcttcaccag ggattcggcc gccacggcca gcatcaggcc
     5821 gtcctggtgc tcggtcgcgt cgatgtgccg agtgccgaac agcactgcga acgccgccag
     5881 cgcaatggcg gcgagcaagg ccgtctcgcc gaagcccgcc acgcctgccc attcctccgg
     5941 caggtgcagg ttgccgacga gcaatgcctc cgtggagatc acgacggcct tcagctgcag
     6001 ggcgatgtag ggcagcgtgc cgacgaccgc cacggcggtc acgacggcag ccaccgcctg
     6061 gctcttgccg tagcgcgcgg cgaggaagtc tgcgatggac gtgatgtttt ggcttttcga
     6121 caaccgcacg atccgcagga tcagcggcca gccgaatgcg aacatcagga tcggcccgac
     6181 gtagacgggg atgaagtcat agcccgtcga ggccgcaagg ccgacgctgc cgaaaaaggt
     6241 ccaggacgta cagtagaccc cgagcgagag cgcgtagatg agcggccgcc cctggccgcc
     6301 ttttcgtgag cggatcgtgc ggtcgccggt ccaggctatg gcgaacagca cgcagatgta
     6361 ggcgataccg agcgccagaa tggcccaacc gtcgaacatg cgcgccggtc cttatgcccg
     6421 ggacagacct tgatccgtgc gcgctaggcc aatggggctg ctgagcgcac gtcagcttga
     6481 tttgcacaaa atttgggctc cagcgcaata cctggagtgg aacggcatca ctggccaagt
     6541 attcacagct acgctcgcca gtgtcgcgag ccgatgattt acagcctgcc gccccaggac
     6601 tagcgttcga ttcacgcttt atcttgatcg gctgtgactc ttggagcggc aggccaaatg
     6661 ggaggtcgaa caatgggcat catcgatgaa ttcaagcaat ttgcgatgcg aggcaacgtt
     6721 ttggacctcg cgattgcggt catcatgggc gccgcgttcg gaccggtcgt gacgacgctg
     6781 gtcgacggca tcatcatgcc gcctatcggc tacattctgg cggggatcga cttctcgcag
     6841 ctctccctga ccttgccgac accgacaggc gacggcgtcg agatcaaata cgggctgttc
     6901 ctgaacgcca tcatcacctt cctgatcacg gcctttgccg ttttcctgct ggtaaaagcg
     6961 gtgaaccgct tccgcaagcc ggcacccgcc gccccgaagg tcgagccgcc caccgaggct
     7021 taccttcgtg agattcggga cgcgctggtg aaacggcagc cgtgaaggac taggagcagc
     7081 ttccgcctcc gagcacgcag aacttggcgc agtgcgggtg gcacagcttg acggcgcctt
     7141 gtgaaaacat cccgccgtct gccttggccg cctctgccag tgtgcggccc atttcaaacg
     7201 ccggctcata gggcagaagc gtgcagggca cgacgacggg gctcgcggca cccttccgct
     7261 tcacgatcat ccggctggac gcgcacatca cgtcgccagg ggatttgcca aggatgcccc
     7321 agcaggcggt cgtaatctcc ggcacttcgc ggtggccgtc catctccggc agcagtgtga
     7381 gctggctggg gtcttgtgca tcgatcggcc aaccccgttc ggcgatcagg cgctggtagc
     7441 cggctctcgc agctggctcg tcctcgcccc agcaggtccg gccggcgatt gcgatgttga
     7501 accggttggc cgcgagccag tcgagccctt ccagtgtctt gctccaggtc cggggtccgc
     7561 gctcgctctc atgccgtgcc ttcgtgtagt ggtcgaggct gacccggatc ttaagccgcg
     7621 acccgtgagc ggcaagcaga gcgaggaggc ccgccttgac cttcggccgc tgcatcgggc
     7681 gcatggcatt ggtcagaacc agcacgcgaa gcccacgtgc cagcgcatcg cctaccatgg
     7741 ggacgatttc gggattgaga aacggctcgc cgccagtgaa cccgatttcg cgggtcggca
     7801 gcttcagctc tgcgatttca tcgagatacg ccgcgacctc cgccgcgctg atgtactcca
     7861 gcctgtcgtt cctcgggctc gattcgatgt agcaattccg gcactggatg ttgcagagcg
     7921 tgccggtgtt gatccacagc gtctcgagcc ctgtcagcgg aacgacagcc cggcgctcgc
     7981 cggtggcagt ccagtccggg tcctcgaact tgcctatggg acgggggcgg cgggcgaagg
     8041 gtttctcaac gagggtcacg cgcttccttt gatcgatcag gcactccaaa caacgctagc
     8101 acggcgcctc ggcgcaaccc actcacctcc ggatcaatcc tgtgcgatcc aagcggggcg
     8161 ggccacatac tcgtgcctgg agtgtgcaac gcgcccccgc gtgtcattca ttcgtccgat
     8221 gcggcgctct cctcggtctc ctcccaatcg ccctcatcct cctcttcctc gacctcgtcc
     8281 gccaaatcct cgtcgagctc ctgctccagc gtgaggatca gggcatcggc aatgagctcc
     8341 agcgccagat gggtcctctg gtcaggctgc gccttcttgt tttcctccga gcgcttcttg
     8401 agctcctcga tgtgttcgaa cgtgactggc atgtgctgag cctctcgtga gcggttcatt
     8461 gtccgcatca ggctatcctg attcaggggg caagggcgag tgggcgagga gttgggcaga
     8521 ccccggcgat cctgtcacgc aagcctcttg cacctcggcg aggagccgac cattatacgg
     8581 gcctccatag agtcccagcc ttcgctgccg gcggaggtgc cgagggatgc gggcgtagtt
     8641 cagtggtaga acatcagctt cccaagctga gtgtcgaggg ttcgattccc ttcgcccgct
     8701 ccaaatttcc agtcaggcgc ctaggctgag ggtccgcctc acggcatccc tccatccggc
     8761 cagcttcctc ttgcggatcg cagcgtccat ggacggttcg aagcggcgtt cgcagcgcca
     8821 gctctcggca aatccttcct cgcccggcca cacgcctgcc tgacggccgg cgagccaggc
     8881 ggcgccgagc gaggtcgtct ccagtacggc cggacggtcg acaggagcat cgaggaggtc
     8941 agcgagccat tgcatcgtga gatcgctcgc gaccatgccg ccgtcgacgc ggaggacggt
     9001 gccggctcca tccgaccagt cgcggcgcat cgcctccacg agatcgcatg tttgatagca
     9061 gacggcctcc agcgccgcgc gggccagctc gttcgggccg gtattgcggg tcaggccata
     9121 gagcgcaccg cgggcgtcag cattccaatg cggagccccg aggccggtga aggcgggaac
     9181 gagatagacg tcctggttgg gatcagccgc tgcggccatc tgggccgatt ggtcagcccg
     9241 ctcgagaatc ttgagcccgt cccgcagcca ctgcacggcc gcaccggcaa cgaagatcga
     9301 gccttccagc gcgtaggtgg tccggccatc gagccggtaa gcgatcgtgg tgagcaggcg
     9361 attgcgcgag ggcacgagat cggcgccggt gttgaggacg gcgaagcagc cggtgccgta
     9421 agtcgatttc atcatgccgg gcgtgaagca ggcctgacct acggtggccg cctgctggtc
     9481 gccggcgatg ccgcggatcg gtatgggcgc gccgaagaga tcggggtcgg tggtgccgaa
     9541 ctcggctgcg gtgtcgcgca cctcgggcag gatcgcacta gggatgcgga agatgcgcag
     9601 cagctccggg tcccatgcct gctgccggat attgaagagc agcgtccgcg aggcattcgt
     9661 gacctcggtc gcatgcactt tgccgccggt caggttccac agcagccagc tgtcgacggt
     9721 gccgaaggca agccggccct gctcggccct cgagcgcgcg ccctcgatcg tatcgaggag
     9781 ccaggcgagc ttcgtggcag agaagtaggg atcgaggagc aggccggtgc gctccgtgat
     9841 ctcttgctcg agcccatccg ccctgagctc agcgcaaacg tccgaggtac gcctgtcctg
     9901 ccagacgatg gcgggaccga cgggcacacc tgtttcgcga tcccacagca gcgtcgtctc
     9961 acgctgattg gtgatgccgc aaccggcaat ctctgaggcc ggcacgccgg acttggcaag
    10021 cgcagtacgc accgtttcga ccgtggtgcg ccagaggtcc tgcgggtcgt gctcgaccca
    10081 gcccgagcgc gggaagtgct gtgtaaactc ctgctggccg aggccgcgaa tgcggtagtc
    10141 cctatcgaac acgatggcgc gggtcgaggt cgtgccctgg tcaatggcga ggatgaaacc
    10201 gctcattcgc gaacctctgc agccgctgtg ggtgaagccg gatctagatt tcgactctag
    10261 ctttacctgc tccagaggtc acggcctccg ggagcgcgtg cgcgagcgat gctccgtctt
    10321 ctcccagaaa tgtggtgccg tcgcgagctg gaagagggcg cggtaggcgg caacggaaat
    10381 cagcagccag tacaccggca tgatcggagc acggaacgcc agccatttcc gcccgcgccg
    10441 ccaggccgcc acgccggcgg ccaagactga gctggcatag gccgcggcga gctcgaatac
    10501 ggtgagggtc cataacgcgc cgccggacag cgactccggc agcatcagcc agccgccctg
    10561 ccacgcctcg aacgcggcca tgccgcagaa cagaggaaac accagtgccg acaggatcaa
    10621 tccgccggag tagaggtgga agccgacgaa tgcggcgaag ccgagctcac gcatgagccg
    10681 aaccggctgg cgcgtgtgaa ccaaatacgt ctgcatccac cctttcatcc aacgggtccg
    10741 ctgccgcagc cagatgccga aacgagcggg agcctcctcc caggtcgtcg agttcaatac
    10801 cccgatgcgg ccgcccgtgc gcgccagcct gatgccgagg tcggcgtcct ccgtcacgtt
    10861 gaagggatcc cacccgaacc atcgatccag cacctcgcgc gggaaatggt ttgatgtgcc
    10921 gccgagcggt attggcaagc cgagccgcgc caagccaggc agggtcacgt cgaacagagc
    10981 ggaatattcc agcgcgaact gccgtgtcag ccagctttcg cgagcattat ggatgttgag
    11041 ctgcccctga aggcagatcg taccgcgcgg agcctcgcgg aacgtggcga tggccagacg
    11101 gagctgatcc ggctccggca tgtcctccgc atcatacacc acgacgtaat cgccacgggc
    11161 gaactgcagg gcgtagttga gcgccttggg tttggtgcgg ggctgcgagt ccggcacgac
    11221 gatggtgcgc atgaagcccg gtagctcgat cgaccgggcg atgtcctgcg tctcgaggtc
    11281 gacgctttcg aggatcagca gcacgtcgag cttggcggcc gggtagtcga gtgcggagat
    11341 tgccgccacg agatcgggca gaacctgggc ctcccggaac atcgggacga ggatcgagta
    11401 ggtgggaaga tcggcatcgg ccagatgggt gctggcgtcg gatgagcgtt ggggcatcag
    11461 gccaagcgcc gtcaccacca cccggagcac cacgaccggc aggaacacga gtgttagcag
    11521 gaccaggaga aggcgatagc ccgcatctgg cgcgaccgtc gtgacgccgg caaccaaccc
    11581 gctcagggcc gacgcggtga tgagttgcca agtccaggtc ggagcacccg cagaaagaac
    11641 tgggcgccag cgcagcaatc ccgcgatggc ccgcttgagc cgccattgct ctcgctctga
    11701 ggagtcccgc tcctccatct cgcgggctgt ggcaatcact gcgatgccgc ctgccgattc
    11761 gcgttcggcg atctgccggg ccacctgccc gggcgaccgg gacgtcgcat caatgagaat
    11821 agcctcgtcc ggcagctcag ccccgaactg gcccaaggcg ggcacgccca aataatctgc
    11881 cagggccccg acgtaggcca catccgtgac ccatccgcgc gccagcagaa cctcgagcgg
    11941 gctgacgccc cagctttccg ccgtcaaaat ggcctgtcgc agcgtttccc ggtcgagcag
    12001 ccggccgatg aggaagccgt actccgcctc ggcggcgaca aggtcttgtt gccggccctc
    12061 ccagtcgaag gacctttggt tatggtagtt cacagcaggc gagcaggcgg gttccgctcg
    12121 gaacgcgttg tcgacgaaac ggcagcagac taagtgttgg gaacgggagg cgggcagcta
    12181 ggtatgatcc taggttggtg caatgccctg ctgcgttgcg agtcgcgagt gatcgaaaag
    12241 cgggcctgaa gatgaagcga cctttccagt tcctcgctgc cgtgctgtcc gttctgctcc
    12301 tcgccggggt gaatcaggtg gtggcgcagg aacctgaccc ggacaatccc gcttcggacc
    12361 agggggcgcg ccggctcgtg ctgcgcttcg tcaccgacgg cgactttccc cccttcaact
    12421 acttcgacga ggatggaatt ctgacaggct tcaacatcga tcttgcccgc gcgctttgct
    12481 tagaagccaa cacctcctgc gacatccagg cgcggccctg ggatacgctg atcccggctc
    12541 tcgaacgggg cgatgcggat gcggtcatcg cgggccacag gatcacgcct cagctcttgc
    12601 agacggtcga tttcaccgag cgctacttcc gaactccagc gcggtttgcc gcccggcgcg
    12661 attcggagaa gatcgagatc agccccgagg agctcgacgg caaatccatc gccgtcgcca
    12721 agggcaccgc tcatagcgct tatctcaagg ccttctttcg gttcagcaaa atcagggagt
    12781 atgacagccc cgagcaggcg cgcgaagcag tgatgaacgg caaggccgac ttcgtgttcg
    12841 acgacggcat cggcctcagc ttctgggtgg tcggcacgat ttcgcacgaa tgctgcgagc
    12901 tgaggggcgg tgcttactat gagccgaaat acttcggcga cggcctcgcg atcgccgtgt
    12961 cgaaacgtga cccgaacctc aagcatctgc tcaacgacgc cttgaagcgt gtccgcgcca
    13021 gcggccgata cgaggagctg gtgctgcgct atttccccaa tcgcgtctat tgatcggcaa
    13081 ggctcgcggt ccaaggattg cccgcgtgcg gatggtgggc cattctgggc gctggcgcca
    13141 tcggtgcctt ccacctgcag tcacgccgct catgagggaa gctttatgtc atcgcgtttg
    13201 ttgccgggcc gcgaggagct cgtcatctgc ttcgcccacg tcgcgtatcg gatgggcgag
    13261 gccttcgcgc gccgaaacac tgggataagc tggcttgagg tccgttcggc ggaggagctg
    13321 caggaggcgg ctgcaaaggc tcatgtaatc gtcacgtccg ggttctggcg caacgagctg
    13381 cttgatgcgg catcgaacct tcgcttcgtg cagtcgatca gtgcagggac ggaccagtac
    13441 gacaaggagc gcttccgcga gaaaggcgtt cggcttgcga gcgccaaggg cgtcaacgcc
    13501 aacgcggtcg ccgagcacgc gatggcgctc atcctggctt tgtcgcgcca gctccatacc
    13561 gggcgcgatc accagaagga gaggcactgg cgcggcatga tcggcgatcc ctcgctgcgc
    13621 gaggatgagc tcggcggcaa gacgttgctg atcgtcgggc ttggcagcat cggtgcgcgc
    13681 ctcgcacggc ttgcgaaggc gttcgacctc aacgtcatcg cgacaaagcg cgatccgagc
    13741 agtggcggcg atgtcgccga cagcgtccat ccccaaagcc agctcaagga tttgttgccg
    13801 cgggccgaca tcgtcgcgct cacgtgcccg ctgacgcccg agaccgaaaa cctgatcgat
    13861 gccaaggcgt tgtcgctgat gaaaacctct gcgcacctga tcaacgtggc gcgcggcagg
    13921 gtggtcgacg aggacgcgct cataacagcg ctccagaccg ggcagatcgc cgctgcgggc
    13981 atcgacgtga cacgcgagga gccgctgccg ccgtcctcgc ccctatgggc catgcccaac
    14041 gtgctgttga cgccgcatac ggcgggcgag acccggcgct atgaagacaa tgtgctcgac
    14101 atcatgatcg aaaacatcga gcgcctgtgg cgcggtgagg cgaccttgcg caacgaggtc
    14161 gtctagaccg gggaaaaatg gtaggccttg gccgctgtcc ccccgttcgc gacgcgaccg
    14221 cgacttgcgg cgtgcgcgcc ggcttccttg cattggaaaa atgccttgag tctccgctgt
    14281 ttggtcgcgc gtcaaggaaa accataataa gttgtttacc ctgcccgcca agctgctaca
    14341 gtgatcgtca agtccaacca gacgtcgcct gatgactgca tactctccga cggcggatgt
    14401 cttcgccagc ggagcctaat ccaggcgctg gggaagggtc gtggaggtgt caaccgacga
    14461 cagggagcca ctccggccgg ccgaggatct ggcggctcct gacaatattg gccggggctc
    14521 accagaagcg gtcgcaatcg cgcccgttga gcgcccctcg accatggttc cgccgtgggc
    14581 cggttcgaaa cccggctttg cgctgcgccc ttcccgcgaa tcgcatcccc gtgacagtcg
    14641 cgtctatgcg gcgctcgatc tcggcaccaa caactgtcgt ctcctggtcg cgcggccatc
    14701 ccgtcggggc tttcaggtgg tggatgcttt ctcgcgtatc attcggctcg gcgagggcgt
    14761 ggggacgacg gggtgtttgt cggaagcggc gatgtcgcgc acgatcgacg cgctcaaggt
    14821 ctgcgcggcc aagatgcggc gtcacaacgt cgttcggtcg cgactcgttg cgacggaggc
    14881 ctgccgagtg gcgaagaacg gcccggagtt catctcccgt gtcagccgcc agctcgggct
    14941 cgagatcgac atcctgtcgc gcgaggatga ggcgagactg gcagtatcgg gctgtgcaac
    15001 ccttctcgac gaccgctcgg atctcgcgct cgtgttcgac atcggcggcg gctcctctga
    15061 gctggtatgg ctcgacctca cccgccggcg ccggagctgg cggcgatccc tgggcgaccg
    15121 cctggatgcc atgaactgca tcgccgcgtg gacatccttg cccgtgggcg tggtcacatt
    15181 ggcggaacgc tacggtggcc ggcagctcac ggccgcagga tttgaggcca tggtcgatac
    15241 ggtgctcgaa ctgctggccc ccttcgaggc caaacactgt ttcaaggcgc aagtcgagcg
    15301 tgggcgcgcc catttgctcg ggacctcggg cacggtcacg acgatggccg gcatcaggct
    15361 cggtttgccg cgctatgatc gcaaccgcgt cgatgggtgc tggctcgatg ccgccgaggc
    15421 gcgcgccatc acctatgatc tgctggcccg ttcgcacgag cagcgcgtcg ccgagccctg
    15481 catcggcaag gaccgggcag atctcgtgct cgcgggctgc gcgatcctgg agggcctgct
    15541 caggatgtgg cccgcgaagc ggctccgtgt tgccgatcgg gggctgcgag agggtatgct
    15601 cgccaccatg atggcggagg acggctgcta tcgcgcattc gaccgccgcg ctcgccggcc
    15661 gaactgaccg gattggccgc tggcgcccga cgcgctagaa ggccccatgg tcaaatcacc
    15721 aaaacaaggc agcggcctcg gcgggcagcg ccagctcaag gtccgcctga agagcggcaa
    15781 gcggcgtacg gcatcgtcac ggcgttggct cgagcgccag ctcagggacc cttacgtcgc
    15841 cgctgcgcag cgcgagggct atcgctcgcg tgcggcctac aagctgatcg aaatcgacga
    15901 caagtacggt tttctgaagc ccggccagcg ggtggtcgac ctgggcgcgg caccaggcgg
    15961 ctggagccag gtggcggcgg ctcgcgtcaa gtcggtcgag gggcacgggc aggtggtcgc
    16021 catcgatatt tcagagatgg agcctcttgc gggcgttgaa gtcctgcatc tcgatttcat
    16081 ggatccgtcc gcgcctgacg cattgaaggc gcgcctcagg gacggcggtg cggatgtcgt
    16141 gctctcggac atggcggcac cgaccgtcgg ccatgcgaag accgatcacc tccgcatcat
    16201 ggcgctcgcc gaagccgctg cggagtttgc ccgcgaggtg ctttcccctg gcggcgtttt
    16261 cctcgccaag gtcttccaag gcggcaccga gggagagttg ctggcttccc tgaagcggga
    16321 tttcgccaag gtgcgccacg tcaagccgcc tgccagccgg tcggatagtg ccgagctcta
    16381 tgtgctggcg atcggcttcc gcggtaatgg ccattcgacg ggagcgcgct agcgccgctg
    16441 gacgagcagc aggcgagcac cacgcgccaa ctccagggtg ctgtcatcca gctccaggga
    16501 gctgctattt ggcccaggct gtgggcagca cgcattggct ggggactgcg gcagtcgttc
    16561 gccgcccgtg acggtgcgtt cgcttccttt gcgccccctc gcgtgctatg cgcgttccga
    16621 aatcgtcccg cggacgcgga ccatttcact catccaggag ccacccatat ggctgcacgc
    16681 ccgatcgaga tcgtcgagaa cgtcgggctg acgttcgacg acgttctgct ggtcccccaa
    16741 tcctctgaaa ctttgcccgg gcaggtcgac gtctccaccc ggatcaccaa aacggtcacg
    16801 ctcaatatcc cgatcctctc ctcggccatg gacacggtca ccgagtcgcg cctggccatc
    16861 gccatggcgc aggccggcgg gatcggcgta atccaccgca acctgacgcc ggagcagcag
    16921 gccaaggagg tcgaggcggt caagaagttc gaaagcggca tggtggtcaa tccgctgacc
    16981 attcatccga atgccacgct caaggatgcc ctcgacctga tgaaggagta ccggatctcc
    17041 ggcattccgg tggtcgagtc ggaccgcagc gggcgcggcg gaaagctggt gggtattctc
    17101 acgcaccgcg acgtgcggtt cgcgaccaat ccggaaacgc ccgtttccga gctgatgacg
    17161 aaggatcggc tcgtcaccgt caaagcgggc gtcggccgcg aggaggcgaa gcggctgctg
    17221 caccagcacc gtatcgagaa gcttctggtc gtcgatgacg agtaccgctg tgttggcctg
    17281 atcaccgtga aggatatcga gaaggcgacg ctgttcccga acgcctgcaa ggacgagcag
    17341 ggccggctgc gggtcgcagc cgcgacgacg gttggcgatg acggacacga gcggacggag
    17401 cggctgattg cggcgggctg cgatgtcgtc gttgtcgaca cggcgcacgg ccactcgcgc
    17461 cgggtcctcg acgcggtgac gcggatcaag cggctctcca acagcacgca ggtcattgcc
    17521 ggcaacgtgg cgacagcgga agcgacacgc gcgctcatcg acgccggcgc ggacgcggtc
    17581 aaagtcggca tcggccccgg ctcgatctgc acgacgcggg tcgtggcggg ggtcggcgtc
    17641 ccgcagctca cggcgatcct cgattgtgtc gccgaggcgc gaaagtccgg cacccctgtc
    17701 atcgccgacg gcggggtgaa gttttcaggt gatattacga aggcgctcgc ggccggtgcc
    17761 gatgcggtca tgatcggctc tctgcttgcg ggcaccgacg agagcccggg cgagacctac
    17821 ctctaccagg ggcggaccta caaggcttac cgcggcatgg gctcggtcgg agccatggcg
    17881 cgcggcagcg cggaccgcta cttccagcag gaggtaaagg acacgctgaa gctcgtgccg
    17941 gagggcatcg aagggcaggt gccctataag ggcccggtca gcacggtgct gcaccagctc
    18001 gtcggcggct tgcgtgccgg catgggatat gtcggagcca ggagcctcgc cgagctgcag
    18061 gcgcgcgcga agttcgtgcg catctcgccg gccgccatcc gcgagagcca tacgcacggc
    18121 gtcggtatca cgcgcgagag cccgaactac ccgggcaatg gctgagcgag gcttctcagc
    18181 gcaggtgctg ctgaccggcc aattgctcgt caggtggggc gatggctgcg cgcgttgcgc
    18241 gcgcaagttc gggcctcgct ctccgttgtc acgtcgcaga ctgggattta gccgatgcgg
    18301 cccggcgcac gaatgaaggc tgccgtggag gtgctggacg agattttcgt ccgccaccgg
    18361 cccgcggcgc aagccttggc cgattggggc aaggcgcacc ggttcgccgg atcgggcgac
    18421 cgcgccgcga tcggcaatct cgtctacgac gtcctccgtc gccggctctc gttggcggca
    18481 cgcatgagcg atgagtcggc ccgcgcgctc gttctcgccg cggcatccga cgcgctgggc
    18541 ctgacgcccg acgagatcgc cgccgcggct gacggctcgg aatatgcact ggcgccgctc
    18601 agcgaggccg agcgcgcggg cctcgtgcgt gacgtgccct ccgatgcgcc gatgcacgtt
    18661 caggccgaca tccccgaatg gctcgcgccc tcgttcgagc ggctgttcgc agaccgcgcc
    18721 gtcgaggagg gcagggcgct cgcacagcgc gcaccggtcg atctcagagt gaatacgttg
    18781 aaggctgatc gcgcgcgcgt cttgaaggcg ctggcccgtt atggcgccga gccgacaccc
    18841 ttgtcgcccg tcgggattcg cattccccca cccaccggcc ccggccggac gccgaatgtg
    18901 gaggccgagc caggacacgc aaaaggctgg ttcgagatcc aggacgaggc gagccagatc
    18961 gccgctctgc tcgccggcgc cagcccgcgc ctgcaagtgc tggatctgtg cgccggagcc
    19021 ggcggcaaga cgctggccat ggcggctgcg atgcagaata cggggcaggt ctacgcctac
    19081 gatgcggacc gcatgcaact tcggccgata ttcgagcggc tgaggcgcgc cggggtgcgc
    19141 aacgtccagg tcctcccggc aggcgacgag ggcgcggtca aggcactcgg cccccgattc
    19201 gacgtcgtgt tcgtcgatgc gccttgtacg ggctctggtg tctggcgacg acgtccggac
    19261 tcgaagtggc gcgtgaaacc gcaaaacctc gccgaccggc agaaggagca gcaggaggtg
    19321 ctggagctcg gcgcaccgct ggtgaagccg ggtggacgac tggtctatgt gacgtgctcc
    19381 atcctgcccg aggagaacag ggatcaagta tcggacttcc tcgcccgcca ctcagatttt
    19441 tccttgcggc cctatgcgca cgcctgggcc gaaaccatcg gcggcgcgcc gccccggtcc
    19501 gccgacggcg gtacggacgc cctacttttg acaccgaccc gtcacggcac tgacggcttt
    19561 tttatcgcaa ttcttgtgcg caacaagtag ccgcttcgac aatttcactt gaataccgcc
    19621 ccggccgaag ttgtccctca acttgtcact tgcattggtc ttgcaagccg ctaagtagag
    19681 tcacgctgag gccatagggg cctcgtttca tggcctggcc gtacggccgg ctgacggggg
    19741 tttcaagagc acgccctcgc cgtcgatccg gcggcatcga aaggaacttc cgtccatcat
    19801 ggcaatagct tcgaaggtga agcggccgcg gcgcgcgtcg gcccgctctt gcctgggccc
    19861 cgtcgccgat ctcgaggcgc atctcccgca ggagtggtgg cgcaagctct tcaatgccct
    19921 ctacgtgaag accgacggcg acgtcgtcga gaacatcgag aacacccggc gcgaggtcga
    19981 cttcatcgtg tcggccgccg cgatccaccc gcacagcgcg atcctcgacc tgtgctgcgg
    20041 gcagggccgt cattgcctgg agctggccag gcgcggcttc aggaatgtca ccggtgtcga
    20101 ccggtcccgc tatctcatcc gccttgcgcg caagcgtgcc cagggtgaag gccttcaggt
    20161 cgtcttcaag gaaggtgacg cgcggaaccc gcggctgccg gagagcagct tcgattgcgt
    20221 cgccatcatg ggcaactcgt tcggctattt ctcgaacaag caggatgacg agaaggtgct
    20281 gacgaccgtc ggcaagatcc tgcgcccgac gggccagctc gtgctcgaca tcaccgacgg
    20341 cgcctacatg gccgagcact tcgagcgccg ctcgtgggag tggatcgacg agcaccattt
    20401 cgtctgccgc gagcgctcca tcagccagga ccgcgagcgc ctcataagcc gcgaggtgat
    20461 cgtccacgac gagatgggcg ttatcgccga tcagttctac gccgagcggc tctacaccaa
    20521 ggagagcatc gccaagctcc tggcgaagac cggattccgc aacgtccgct atcacggtca
    20581 cgccgaggcg ctgtccgacc gcgaccagga cctcggcatg atggcgcgcc gcatcctgct
    20641 gacggcagat gccccgcagc gtccggcccg caagagtcgc ggcaaggtgc agaagctcga
    20701 tgtcaccgtg ctgctcggcg acccgcggct gccggatgac gtcaagcgcg gcggcacctt
    20761 caatccggag gacctggaga ccatccgcaa gctcaaggac gcgctgtcga cgctgccgaa
    20821 ctacacgttc cgctatctcg acaaccacgc caccctggag cgcgacctgg cgggcctcag
    20881 gacggacctc gtgttcaatc tctgcgacga ggggctcaac aacgatccgt tcaaggagct
    20941 gcacgttccg gcgatgctgg aggtgctggg gctcccctac acgggcgcgg cgccgcaggc
    21001 gctcgcggcc tgctacgaca agggtctcgt gcgcgcggtg gcgatgacgc tcgacgtgcc
    21061 ggtgccgctc gaaacctacg tgcgcccggg cgatcagggc gccacgctgc cgtcggtgtt
    21121 cccggccttg ctcaagccca actacggcga cagctcacag ggcatcacca aggacgcggt
    21181 ggtgaccaac gagcgggcgc tgctcgacta cctggagcgg ctcagggccg agttcccgcg
    21241 ccggccgatt ctggtgcagg agtatctgac ggggccggag tattcggtga gcctcatcgg
    21301 caatccggac cagggcctac gcgcgctgcc tatcctggag gtcgactact cgcggcttga
    21361 tccgagcttg ccgaaaattc tcggctacga gtcgaaatgg gagccggaca gcccctactg
    21421 gacgcagatt cgctatcacg aggcgaacct gcccgatcac atccaggcgc agctcatcga
    21481 gcactcggcg cggctcttcg agcggctcgg ctgccgtgat tacgcgcgat ttgatttccg
    21541 cgccgatgcc cacggcgaga tcaagctgct ggaggtcaac ccgaacccgg gttggtgctg
    21601 ggacggcaag ctcaacatca tggccggctt ccagggcatg cgctacgcgg agcttctggg
    21661 ccagatcctc gccgcggcgg aggagcgcct gggcatcgtc ccgaagccgc acgcactgac
    21721 cgtcggcgcc gcgccgaacg gcgtgcatgg cggggcgcag gcacattcca atgcggtcat
    21781 ggcgccacag atgaagtagt agactactgg ctggcagctc ggcgatcagc gtgagctcgc
    21841 gcccaatatc cctctccccg gtgggaagag ggtcggggtg aggggggggc cgccggtagc
    21901 ggctgttgta acccctcacc cggcccttcg ggccgacctc cccctatggg agaggttaac
    21961 ggagtgcttg ctgttcacgt ttccagctga atcggaaaag ctctaagcga tctccctggc
    22021 gcgctcgcgc agcacgaatt tctggatctt ccccgtcgag gtctttggca gcggtccgaa
    22081 tacgaccgtc ttcggcacct tgaagccggc gacgttcgcc cggcagtagg caatgatgtc
    22141 ctcggccgtg gtcggctcag cgtcgggctt cagggtcacg aaggcgcaag gcgtctcccc
    22201 ccatttcggg tcgggcttcg cgacgacggc agcctccatg atctgcggat ggcgatagag
    22261 aacctcctcc agctccagcg aggagatatt ctcgccgccc gaaatgatga tgtccttcga
    22321 gcggtccttg acctcgatgt agccgtccgg gtgccacacc gcgaggtcgc cggagtgata
    22381 gaggtcgccg gcgaaagctt catccgtggc cctcggattc ttgagatacc ccttcatcag
    22441 ggtgttgccg cggagcatga tctcaccgat ggcttggcag tcgcgcgcga cgggcccgag
    22501 ggtgaccggg tcggcgacgg tcatgtcctc gatcagcacg tgcgggatgc cctgccgcgc
    22561 catgttggtg aaccgctgct cctcgggccc attgtcccag tcgcgcatgt gcacgcaggt
    22621 cgtcgacggg ccgtagctct ccgtcgtgcc gtaggcgtgc agcacctcga aacccatggc
    22681 ctccatgttg cggatgacgg ccgatggcgg ggcggcgccg ccggtcatca ccttcgggcg
    22741 cgtcggcagc ggccgtttcg tctctgccgg cgcgtggatg agggtgttga gcacgatcgg
    22801 cgcgccgcac atgtgcgtga cctcgtgctc ggcgatcagg tcgaagatgc gcttcggctc
    22861 gatcttccgt aggcagatgt gcgtgccgac cgcagcggtg accgcccacg tgtaggtcca
    22921 cccggagcag tggaacatgg gcagcgtcca gaggtagcgg ctgttgtggt cgagcttgaa
    22981 ggtgagcgcg ttggcaagcg cattgaggtg cgcgccgcga tggctgtaga cggcgccttt
    23041 cggattgccg gtggtgccgg aggtgtagag aaggcagatg gaatcccatt cgtcctcggg
    23101 accgggatag gcgaaggccg agtcgccggt ggcgagaaag tcctcgtact ccacgctgcc
    23161 gaacgcgggc gcctcgggtg cttcccaatc ggcgatgtcg atgacgagcg gtttcgtctc
    23221 gagcctttcg agcgccggca atacgctgct cgcgaactcg cgatcggcca gcaggacctt
    23281 cgcctcgccg tgctggaggc agaaagcgat cgtcgccggg tcgaggcgga tgttgatcgg
    23341 attgagcacc gcgccgatca tcggcacggc gtagtgcgcc tccagcaatg ccggggtatt
    23401 cggcgccaga atcgcgaccg tctcaccgcg gccgacgccg gccttggtga gcgcatgggc
    23461 gagccggcgc gagcgggcgt agagatcggc atacgtgaag cggcgcgcgc catgaatgac
    23521 ggccgtctgg ttcgcgaaga agttggccgc gcgtgctaga aagctcaccg gagtgagcgg
    23581 cacgaaattg gccggacgct tcctgagctc tggtcgctcc tcaaccatgg gcatttcctc
    23641 tggccaccac gtcctctgac ggggagcttg catttcgaag caaaatggcc agcccccgcc
    23701 ataatttcac tcccgcccct gacgggatct caaaaaccgc gggttccgga tacgcagctc
    23761 ctccacgagg atggcgcgga gtcgctggtg cagatccggt acggtgttag tgctgacctt
    23821 gccggagcga atgtcaagcg cgagctgctt catgtctccg tccccttcgg ggtagagctc
    23881 gtcgagcaat tcccagcgag ccgtttcgcc gtccgtcagg atctcacggc gcgcgatctc
    23941 cagcgcgttg gcgatcatgg cggcggtgta gcggtgctca ggcggcaggc tgggttggat
    24001 ttcccctctc agcgtttcca ccgccagctc gatcagcgtc tccgccgaaa tgcgtttcac
    24061 cgccatcgcg gatcccccga tcccatcagc gcggcgatgc catcgagcgc atcgagctcc
    24121 atttccgagg gcatgaggcc ggtgagcgcc agctcgatgg agttctcgcc gcctttgcgg
    24181 tagcggtcgc cctgcagcac ggcgacggac gcccatttgg cggcggccat gatctcccag
    24241 tacgggatga gcgactcgtc gaagggtaat gccgctgcgc tccggtagcc gcggtagaac
    24301 gcctcccggc tggcgatgcc gccggtttcg agggcgtcgc tcccgaagcg ccagcagcgg
    24361 gccatgatcc agccgatgtc ctcgtgccgg tcgccccaat gggcgaactc ccagtcgagc
    24421 acggcgccga gccggccttc gctcacgatg tagtttcccg tgcggaaatc tccgtggacc
    24481 agcgtgatcg gctcgggctt cggcgcgtgc gcatcaagcc aggccagcac gtactccaag
    24541 gccggccgcg gctctgtcgc agggctcagc gcattgcgca gtcgggccac ctcgttccgc
    24601 gccggcgcca gcatcggaat cggcagaaag ctcaggtcgt cccgcggcgg tgtgatgcta
    24661 tggattttcg ccagctccgc gccaaggctc tcggccaggc tctcgccgaa atcgctgaga
    24721 gcggggtccc gcacgatgcg ccgcgcctga gccacacccg gcacgaacgc ctggatcaga
    24781 aacggcgcgc cgatgacgtc cgcatcggca cagcgcgcga tcggctccgc aacggccacg
    24841 ccggctgcgt gcgctgcctt gaggacgcca aactcgcctg cgcgat
//
LOCUS       DASVDU010000242         8006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_286892, whole genome shotgun sequence.
ACCESSION   DASVDU010000242 DASVDU010000000
VERSION     DASVDU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286892"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..614)
                     /locus_tag="VF226_15615"
     CDS             complement(195..614)
                     /locus_tag="VF226_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX7075466.1"
                     /translation="MAGNVYEMRPKTTDSEKITINLGFVDLGHIDLLVQEGFYSNRTD
                     FIRTAIRNQIERHADVVRQAVTRKSVDLGLRHFSREDLEAARDAGQMLDIRVLGLASI
                     AQDVTPELARAAIASLSVLGALHASAAVKTALADRIR"
     gene            995..1441
                     /locus_tag="VF226_15620"
     CDS             995..1441
                     /locus_tag="VF226_15620"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX7075467.1"
                     /translation="MEIFELLKQDHDEAKALLTQICESEDGKERGELFKELRKKLLAH
                     AHAEQKVLYEPMKKQEETKDIALEAVVEHEVVERLVEDLSRSRSRDSDAWLARATVLK
                     ETLEHHIKEEEGEMFEKARSVFDGKTLEKMAEQIQKAKERELKKAA"
     gene            complement(1631..3424)
                     /locus_tag="VF226_15625"
     CDS             complement(1631..3424)
                     /locus_tag="VF226_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX7075468.1"
                     /translation="MFTTRPEINGTFGVVTSTHWLATAAGMAILERGGNAFDAAVATG
                     TSLHVVEPHQNGPGGEVPLILHSADRDEVKVICGQGVAPDAATIETFQHLGLDVVPGN
                     GLLSAVVPGSFGAWMLLLAEYGTLTLRDVLAPAITYAREGFYLVPNAATIIGNAEKLF
                     RTEWMSSAEVWLPGGEVPRPGTRFRRPHLASTFERIVRDAEAESGSRLGQIEAARRIW
                     YEGFVAETVDRFFRENALMDTSGERHSGLLTGDDMARWRATIEDPVTYEYEQFTVCKA
                     GPWTQGPVFLQQLALLKGFDLKAMGPNSADFVHTVVECAKLAFADRDAYYGDPGFVDV
                     PLDALLSDRYNAERRALVGANASMELRPGRVPGIEPHITVRPKGSTPVATPESLDFNY
                     PRPKESLDQPISTGDTTHFDIIDRHGNMISGTPSGSSLHLAPIVAGLGFSMPARAQMF
                     WLEEGLSGSLAPGKRPRTTLTPTLALRDGEPYMAFGTPGGDCQDQWAIQAFLRHVHFD
                     LDLQAAIDAPTFHTYHLVDSFFPREYDPGHLAIEGRFSDEVCRDLRRRGHRLEVYDDY
                     SLGYVTAATRDGDLLKAAASPREMQCYAAGR"
     gene            complement(3424..4179)
                     /locus_tag="VF226_15630"
     CDS             complement(3424..4179)
                     /locus_tag="VF226_15630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7075469.1"
                     /translation="MPIDSHAALPLRDRKKARQRAELLRVAAELFRREGYEGTRMEDI
                     AAQADVSVPTVYNYFAGKRDVLIDLLMQDRRETQTTFEVVVNNPPDEPADAFAALIHA
                     NMANIRSPEDKRLWRDLIAAVAKAHDREQDQFEKNHEIFRGYIKRLLRHFMRCGKLSG
                     KLPLSLAADIIFAINSSNLRYLVAAESCTPEEIQNLARRQMALLMTDWSIGDSRKVAP
                     LSPQMKGSRKPSPRRRATRVVGTTDSSGYQEAD"
     gene            4451..5572
                     /locus_tag="VF226_15635"
     CDS             4451..5572
                     /locus_tag="VF226_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7075470.1"
                     /translation="MEENRFRDRSGRPTMTRRAAIRALASVGVGVAILPLTHTRSTAA
                     EQVSVFTWSSYNDPKLFPGYIEKYGSPPDFSFLADTAEALMKVRAGYAADIAHPCADD
                     ISLWIKADVIQPFDPSRLTHVPDFWDELKDLPGTVSDDGTKRWFVPFDWGNSSIVYRT
                     DMVELKGEPSWSILYTDERYKDKVSMYDSAPPAVNIAAAILGFPDIFILSDEELEEVA
                     KLLRKQRELVKFYWTDQTTVEQGIASGELVAAYAWNDGFKRLKNQGVPVAYMNPKEGM
                     RTWVCGLVLHKDTPHLDMAYDFVNAFTSPEAGAHLLEAFGTGHVNRKAFDLVSQELLD
                     SLGISNPAEMMARTVFLKPTPPEYLQKYQRLFDMVKAGG"
     gene            5635..7392
                     /locus_tag="VF226_15640"
     CDS             5635..7392
                     /locus_tag="VF226_15640"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX7075471.1"
                     /translation="MSGRGDHETPGVIGSGRSTGRPPVIGREAAVSTGHPLATLAAMR
                     ILDGGGNAIDAGVAAGLALGVLQPDIVGCAGVAPIILYLADAAEVVTISGLGRWPRRA
                     DIGFFERHANGELPLGVLRSVVPASIDAWVTALEEFGTRSFADVATAAFELAEQGFAA
                     HELLCTTIAEDQALYAKWPSSAAVFLRDGRPPSVGEPFRQPDLARTIWRLMEAERREG
                     ADRRAGLRAVRKEFYDGAIAEEIAAFYQREGGLLSAQDLREFRVGIEAPVSTSFRGYE
                     VYSCGAWCQGPVLLEFLNMLEDDDFQTIGCNSPDYIHLVTEVMKLGFADREAYFGDPD
                     FVDVPLRRLLDKGYAKDRRREVSLDQAWPAMPPPGDVGRSVEPTRVKPSAGPGHSKGA
                     PDTTYLCVVDKYGNAFSATPSDGYSTAPVLPGLGFAVSPRGDQSWLEPGHASALAPWK
                     RPRLTPNPALALKDGKLALVFGTPGGDVQCQAMLQFLMNTVVFGMNPQAAIETPRFAT
                     YSFPNSFYPHHSEPGMLRLETELRAAACDLEQKGHRVELWPQRFWRAGGLCAIVIDEK
                     TAYRMAGADPRRECYALAW"
     gene            complement(7545..>8006)
                     /locus_tag="VF226_15645"
     CDS             complement(7545..>8006)
                     /locus_tag="VF226_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX7075472.1"
                     /translation="CFNAVAQRGRQRLSGTAQTGPSAATEAWLIYDGECPFCSAYVKY
                     LRVQDSVGALHLVNARDGGPVVEQVKQAGADLDEGMVLKIGDRLYHGADCIHALALLS
                     TPSNIFNRINGRIFRSPTMARHLYPILRAGRNAALRLLGRRKIGRAPQSLP"
BASE COUNT         1534 a         2399 c         2569 g         1504 t
ORIGIN      
        1 gagccgtcgc ctcgatgagt ttgccagcat tggtgaggcg ggtcgcctcg gccatcccgc
       61 ggggaaatcg agaggtcatg tgagtgcttt ctgggcttgg gaacaggctg agcgaccgca
      121 tggggaagtt cagcccgtct cctgtttcgg ggtgtcgtcc gcgctgcggc atcagcggcg
      181 cgcgctggaa gccgtcatcg gatgcggtct gcgagggctg tcttgacggc ggcgctggcg
      241 tgcagcgccc ccaatacgga aagcgaggcg atggcggcac gggcaagttc gggagtgacg
      301 tcctgagcga tgctggcgag acccaggacg cgaatatcga gcatctgccc ggcatcccgt
      361 gccgcctcga gatcctcgcg gctgaaatgc cggaggccga gatccacgct cttgcgtgtc
      421 acggcctgcc ggaccacgtc agcgtgccgc tcgatctggt tgcggatggc ggtccggatg
      481 aagtcggtac ggttcgagta gaacccctcc tgcaccagca ggtcgatgtg gccaagatcg
      541 acaaagccga gattgatcgt aattttctca ctgtccgtgg tcttcggtcg catctcatac
      601 acattgccgg ccatctgctc gccatcccct taccatccat atggatggta tatagagctg
      661 cctgagcatc tttcaaggta tgagacgact cggcccgtct gcatgccgga tgtgcacgga
      721 gccaaatggc gaatccaagg ctgagagtgg gccgagacgg cgccagactg cgatgtcctc
      781 gccgaatagc tcggaaagca ggggcggtgc ccgcgccagg aatgcgagtt cgataatccg
      841 agggaaaggc ggatcgcatg gaccgcggtg ccgccttccg gctgggccga ccgccgccgg
      901 ttgaagttga agaagcctcc agcggaaccg cttccaaagc gcaggcgttc attggccggc
      961 ccaccttggg gttcaacgat tgaggagacg caccgtggag atcttcgaac tgctgaagca
     1021 ggatcacgac gaagcgaagg cgctcttgac gcagatctgc gagtccgagg atggcaagga
     1081 gcgcggcgag ttgttcaagg aacttcggaa gaagctgctc gcccacgcac atgccgagca
     1141 gaaggtgctg tacgagccga tgaagaagca ggaggaaacc aaggacatcg cccttgaggc
     1201 agtggtcgag cacgaagttg tcgaacgtct ggtcgaagat ctctcgcgca gccggtccag
     1261 agacagcgac gcttggctcg cccgggcaac ggtattgaag gagacgctcg aacatcacat
     1321 caaggaagag gaaggggaga tgttcgagaa ggcgcgcagc gtattcgacg gcaaaacctt
     1381 ggagaagatg gccgagcaga ttcagaaggc caaggagaga gaactgaaga aagcagcctg
     1441 atgaagccgc tggagtgacc gaactcggcg gagctcccgc cggatcttcg ctgatctcca
     1501 gatgccggga cgtgaggcaa tatgagttga cggagcggac gcgcctcgta ccagcaaatc
     1561 cagatgccgc cggcgaaata tccgggctaa ggatgcaggg ggttttgctg tggagcgcag
     1621 cggataattg ctaccgaccg gctgcgtaac actgcatttc ccgaggactc gcggctgctt
     1681 tcagcaggtc gccatctctc gttgctgccg tgacgtagcc gagagagtaa tcgtcgtaaa
     1741 cttccaatcg atgacctcgg cgcctgaggt cacggcacac ctcgtcgctg aaacgtcctt
     1801 cgatcgcgag atggcccggg tcgtactctc gcggaaagaa ggagtcgaca aggtgatagg
     1861 tgtgaaacgt cggagcatcg atggcggcct gcagatccag gtcgaaatgg acgtggcgca
     1921 agaacgcctg gatcgcccat tgatcctggc aatcgccgcc cggcgtcccg aacgccatgt
     1981 aaggctctcc atcgcgcagg gcgagcgtcg gcgtgagcgt ggtgcgcggt ctcttgcccg
     2041 gagcgagaga tcctgacagg ccctcttcca accaaaacat ctgagcccgg gccggcatgc
     2101 tgaaaccgag gccggcgacg atgggggcca gatggaggga tgaaccgctc ggtgtgcccg
     2161 agatcatgtt gccgtggcgg tcgatgatat cgaagtgcgt cgtatcaccc gtggagatcg
     2221 gctgatcgag gctctccttg gggcgcggat agttgaaatc cagcgattca ggagtcgcca
     2281 ccggtgtcga gcccttcggc cgcacggtta tgtgcggctc gatgccgggt acgcggcccg
     2341 ggcgcagttc catggatgcg tttgcaccaa ccagcgcccg acgctctgcg ttgtagcgat
     2401 cgctgagaag agcatcgagc ggcacgtcga caaagccggg atcgccgtag taggcgtcgc
     2461 ggtccgcgaa ggcgagtttc gcacattcga cgaccgtatg gacgaagtcg gcgctgttcg
     2521 ggcccatggc cttgagatcg aagcccttca gaagcgcgag ctgctggagg aagacgggac
     2581 cttgcgtcca gggccccgcc ttgcagaccg taaattgctc gtactcgtag gttaccggat
     2641 cttcgattgt ggcgcgccaa cgcgccatgt catccccggt cagcagacca ctgtgccgct
     2701 ctcccgaggt gtccatgagc gcgttctcgc ggaagaagcg atctacggtt tccgcgacga
     2761 agccctcata ccagattcgc cgtgccgctt cgatctgccc cagccggctg ccgctttcgg
     2821 cttcggcgtc acggacaatg cgctcgaacg tgcttgccag atgaggccgg cgaaagcgcg
     2881 ttccgggcct tggaacctcg ccgccgggca gccacacctc ggccgaggac atccactccg
     2941 tgcggaagag cttctcggcg ttgccgatga tcgtcgcggc attcggcacc agatagaagc
     3001 cctcgcgcgc ataggtgatc gcaggcgcca gcacgtctcg cagggtcagg gtgccgtact
     3061 cggccaacag cagcatccat gccccgaacg atccggggac gacagcggaa agcagcccgt
     3121 tgcccggcac gacgtccagg ccaagatgtt ggaaggtctc gatcgtggcg gcgtcgggcg
     3181 cgacaccctg accgcagatg acctttacct cgtcacgatc ggcgctgtgc agaatcagcg
     3241 gcacctcgcc gcccggccca ttctggtgag gctcgacaac atgaagcgaa gtcccggtgg
     3301 cgacggcggc atcgaaggca ttgccgcccc tttccaggat cgccatcccg gccgccgttg
     3361 ccagccaatg ggtcgaggtg acgaccccga aggttccgtt gatctccggt cgggtcgtga
     3421 acatcaatcc gcctcctgat accctgacga atccgtcgtg cccacgactc tcgttgctcg
     3481 tcgccttggg ctgggcttac gcgacccctt catttgggga gagaggggcg ccactttgcg
     3541 ggagtcgccg atagaccagt ccgtcatgag caaagccatt tgccggcgtg cgaggttctg
     3601 gatctcttcg ggggtgcatg attcggccgc gacaaggtat cggaggttgc tcgagttgat
     3661 tgcaaatatg atgtctgccg ccagcgataa cggaagcttg ccggacaact ttccgcatct
     3721 catgaagtgc cgaagcaggc gttttatata ccctctgaag atctcgtgat ttttttcgaa
     3781 ttgatcctgc tcgcgatcgt gcgcctttgc caccgcggca ataaggtctc gccacagccg
     3841 cttatcctcg ggagaccgga tattcgccat gttcgcatgg atcagcgcgg cgaatgcgtc
     3901 ggccggctca tcaggcgggt tattcaccac gacctcgaaa gtggtctgcg tctcgcgccg
     3961 gtcctgcatc aacaggtcga tcagaacgtc gcgcttaccg gcgaagtagt tgtagactgt
     4021 cgggaccgag acatccgctt gcgctgcgat gtcctccatc cgggttccct cgtacccctc
     4081 tcgcctgaac agctcggccg ccacgcgcag tagttcagcg cgttgcctcg ctttcttccg
     4141 gtctctgaga ggcaaggccg catggctgtc aatcggcatg aatcatctcg cttccatatg
     4201 cggagccgtg aggcggcgac gcgccgagaa gggtttcgtt caaagccacc ttacctcccg
     4261 atcgctgggc accgcatgcg cggtgaacat gccgtcctgc atcacatgca ccataccatg
     4321 acctccactt tgacatgcgc agccaaagcg atcatcgcgc ctttccataa cggtgttgac
     4381 gcaaaaaact tatatggatt aatatttttt taccagataa gaattttaaa acagaacagg
     4441 gaaaaacgcc atggaagaga atcgcttccg cgatcgctcg ggtcggccga cgatgacccg
     4501 gcgagccgct atcagggcat tggcgtcggt cggggttgga gtcgccatac tccccctcac
     4561 ccatacgcgg agcaccgccg cagagcaggt gtcagtcttc acctggtcga gctataacga
     4621 tccgaaactg ttcccgggct atatcgagaa gtacggcagt ccgccggact tcagtttcct
     4681 ggccgatacg gcggaagcgc ttatgaaggt ccgcgccggc tatgctgcgg acatcgcgca
     4741 tccctgtgcc gacgacatct cgctttggat caaggcggat gtcattcagc cgttcgatcc
     4801 gtcgcgcctg acgcatgtcc ccgatttctg ggatgaactg aaagaccttc cgggcaccgt
     4861 cagcgacgac ggcacgaagc gctggttcgt gccgttcgac tggggaaact cgtcgatcgt
     4921 ctatcggacc gatatggtcg agcttaaggg ggaaccgtcc tggtcgatcc tctacaccga
     4981 cgagcgttac aaagacaagg tttcgatgta tgactcggca ccgccggcgg tcaacatcgc
     5041 cgcagccatt cttggctttc ccgacatttt tatcctgagt gatgaagaac tggaggaagt
     5101 cgcaaagctc cttcggaagc agcgggagct tgtcaagttc tactggacgg atcagacgac
     5161 ggtagagcag ggaatagcat ccggtgaact cgttgccgct tatgcctgga atgacggatt
     5221 caagagattg aaaaaccagg gcgtgccggt cgcctacatg aacccgaagg agggaatgcg
     5281 cacctgggtt tgcggtctgg ttctccacaa ggacacgccc catctcgaca tggcgtacga
     5341 cttcgtcaat gcgttcacat cccccgaggc cggcgcgcat ctgcttgaag ccttcgggac
     5401 cgggcatgtg aatcgcaagg ccttcgacct tgtcagccaa gagctgcttg actcgctggg
     5461 gatctcaaat ccggccgaga tgatggcgcg gaccgtattt ctgaagccaa ccccgcccga
     5521 gtacctccag aagtaccaga gacttttcga catggtgaag gccggaggct gagcggactt
     5581 caccaacctg cgctcgagca gcgccattgt tgagatagtt caggagccgg tgcggtgtcg
     5641 ggacgtggcg atcacgagac cccgggcgtg attgggagcg ggcggagcac cggccgccct
     5701 cccgtgatcg ggcgagaggc ggccgtgtcc accgggcatc cgctcgcgac gctcgcggcg
     5761 atgcgcattc tcgatggggg cggaaacgcg atcgatgctg gagtcgcggc cggtctggcc
     5821 ctcggggtgc tccagccgga tattgtcgga tgtgcgggcg tcgcaccgat catcctctat
     5881 ttggccgacg ccgcggaggt ggttacgatc agcgggctcg gccgctggcc gcgccgcgcc
     5941 gacatcggtt tctttgagcg acatgcgaac ggcgaactgc ccttaggcgt gttgcgcagc
     6001 gtcgttccag cgtcaatcga cgcctgggtc accgcgcttg aagaattcgg cacgcggtcc
     6061 ttcgcggacg tggcgacggc ggcgttcgag ctggccgaac agggattcgc ggcgcacgag
     6121 ctgctgtgca cgacgattgc cgaggaccag gccctctatg ccaaatggcc gagctccgcc
     6181 gccgttttcc tgcgcgatgg ccggccgccc tcggtcgggg agccgttccg gcagcccgac
     6241 cttgcccgca ccatctggag actgatggag gccgagagac gcgaaggcgc cgatcgccgg
     6301 gccggccttc gtgcggtccg caaggaattc tatgacggcg cgatcgcgga ggagatcgcg
     6361 gcgttctacc agcgtgaagg cgggctgctt tcggcccaag acctgcggga gtttcgggtc
     6421 ggcatcgagg cgcccgtttc gacgagcttc cgcggatacg aggtctatag ctgcggtgcg
     6481 tggtgccagg gacccgttct cctcgagttc ctgaatatgc tcgaggatga tgattttcag
     6541 accataggct gcaattcgcc ggactacatc catctcgtga cggaggtgat gaaattgggt
     6601 ttcgcggaca gggaagccta tttcggcgac ccggacttcg tcgatgtccc gctccggcga
     6661 ctgctcgaca agggctatgc aaaggacagg cgccgagagg tttccctgga ccaggcgtgg
     6721 ccggccatgc cgccgccggg agatgtcggg cgctctgtcg aaccgacgcg cgtgaagccc
     6781 tccgccggtc cgggccattc caagggagcc ccggatacga cctatctttg cgtcgtggac
     6841 aagtacggca atgcgttttc ggcgacgccg agcgacggat attcgacggc tcccgttctg
     6901 ccggggcttg gcttcgccgt gtcgccgcga ggcgaccaat cctggctgga gcctggtcac
     6961 gcgagcgcgc ttgcgccttg gaagcggccg cgcctgacgc ccaatcctgc cctggcactg
     7021 aaggacggaa agcttgccct ggtcttcggc acgcccggcg gcgacgtgca gtgtcaggcc
     7081 atgcttcaat tcttgatgaa taccgtggtg ttcgggatga atccccaggc ggcgattgag
     7141 acgccgcgat ttgcgaccta cagctttccg aactcgtttt atccgcacca cagcgagccc
     7201 ggtatgctgc gactggaaac ggagttgcgc gccgcggcct gcgatctcga acaaaaggga
     7261 catcgggtcg agctttggcc gcaacggttc tggcgggcgg gagggttatg cgcgatcgtc
     7321 atcgatgaaa agacggccta ccgcatggca ggcgccgatc cgcgtcggga gtgctatgcc
     7381 ctggcgtggt gacgagagct tttcatgcgc cgtgccgccc cagggcacat gcgccattgc
     7441 cacgcacaac ctcggacctt acgcggcacg tagacagcgc ggtcacgccg catgacggag
     7501 ggttggtgca ggctcaggac atcgcaatgc cgaccagcgg gacgctacgg cagcgattgc
     7561 ggtgcgcgcc cgatcttccg ccggccaagc agccgcagcg ccgcgttccg gccggcccgc
     7621 aggatcggat acaggtggcg cgccattgtg ggcgagcgaa agattcgccc attgatccga
     7681 ttgaagatgt tggagggggt gctgagcaag gcgagcgcat ggatgcagtc ggccccgtga
     7741 tagagccggt cgccgatctt cagaaccatg ccttcgtcga ggtcggctcc ggcctgcttg
     7801 acctgttcga ccaccggacc accgtcacga gcattcacga gatgaagcgc gccgaccgag
     7861 tcctggaccc gcagatattt tacgtaggcg ctgcagaacg gacactcgcc atcatagatg
     7921 agccaagcct ccgtggccgc tgatggacct gtttgcgctg tacccgacaa tcgttggcgc
     7981 ccccgctgag cgacggcgtt gaaaca
//
LOCUS       DASVDU010000243        19363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1062023, whole genome shotgun sequence.
ACCESSION   DASVDU010000243 DASVDU010000000
VERSION     DASVDU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 19363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19363
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062023"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1793
                     /gene="soxB"
                     /locus_tag="VF226_15650"
     CDS             102..1793
                     /gene="soxB"
                     /locus_tag="VF226_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HEX7075473.1"
                     /translation="MISRRELVQAALIASASIGGSMGSLARALAQGKLSQDALLGFRE
                     LGNVTLVHMADLHAQLVPVWFREPSENFGVGEARGRVPHLTGDDLLKEFGLAPGSPEA
                     YAFSAEDFVELARKYGRMGGLDRIATVIKAIRAERPDRTLVLDGGDTLTGSWTSLKTQ
                     GQDMVDAMSLIAPDATTAHWEFTLGEDRVREITEKLEFPLLAQNVRDTDFEDSVFKAY
                     SLFERGGINIAVVGQAFPFTPIANPRWMIPKWTFGIRDSDLQEQIDAARADGADLVVL
                     LSHNGFDLDRKLASKLDGVDVILTSHTHDALPEVVKVGRTLLIASGSHGKFVARLDLD
                     VRGKEVKDFRFKLIPIFSDVIKPDPEMAALISKHRAPFAEDLARVLGRTNGLLYRRGT
                     FAGTLDDLFCEAMLAERDAEIALSPGIRWGASLLPGQDITFEDVTNACALTYPACYRT
                     TMTGARLKEVLEDVADNIFNPDPYYQQGGDMVRTGGLAFTIDIGASMGSRISDLTLLR
                     TGEPIEAAREYVVAGWASVNEGTEGPPIWDVVSRHVSARKVIDLKPNASVKVVGA"
     gene            1820..3127
                     /gene="soxC"
                     /locus_tag="VF226_15655"
     CDS             1820..3127
                     /gene="soxC"
                     /locus_tag="VF226_15655"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HEX7075474.1"
                     /translation="MTEETTGTGLRSLRLNRRQLIATAAAAGIGAAGARRAYAQDVTG
                     SIAGNPENLPPNVPEWSKVLGDGVDARPYGMPSKFEAHVVRRDVKWLTGSRESSVNFT
                     PLHELDGIITPNGLCFERHHGGVAEINPLEHRLMIHGLVDKPLIFSMEDIKRFPRVNR
                     IHFLECAANSGMEWRGAQLNGCQYTHGMVHNVMYTGVPLKLLLEEAGLKPNAKWLLLE
                     GADSAGMTRSLPIEKALKDTLIAFKMNGEALREEQGYPLRAVVPGWEGNLWVKWLRRI
                     EVGDQPWHTREETSKYTDLLADGLSRRFTYFMDAKSVVTNPSPQAPLIHKGYNVLSGL
                     AWSGRGTITRVDVSLDGGRNWRTARIDGPVLEASLTRFYVEFDWQGEELLIQSRAMDS
                     TGYVQPTKAELRKVRGVNSIYHNNGIQTWLVRADGVTENVEIT"
     gene            3111..3767
                     /locus_tag="VF226_15660"
     CDS             3111..3767
                     /locus_tag="VF226_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7075475.1"
                     /translation="MSKLPSLFIALTLAAAAASPAFGGKFGLGREATPEEIAGWDIDV
                     RPDGQGLPAGKGTVAEGEEIFQTYCAVCHGEFGEGIDRWPALAGGRDSLKNDRPDKTV
                     GSFWPYATTIFDYIRRAMPFGNAQSLSPDEIYALTAYILYLNDITKDPEFELNQANLP
                     SVKMPNAGNFYDDDRETAEKQFWNKDPCMKDCKKTVEILGRARLLDVTPESKTSPRVD
                     "
     gene            complement(3854..4888)
                     /locus_tag="VF226_15665"
     CDS             complement(3854..4888)
                     /locus_tag="VF226_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075476.1"
                     /translation="MHYVPDRRRAFALVLAAILATATSPAISQPPNAVRQEPVPVEVT
                     NGSQPVLCAEKDNVSIAFTSPRVRSFRIEAAHPVYMSNLQRDSFEPDWTDCDMSGDPV
                     FAAPVPPRRVTIYEEIDFWLVGLTFPTFWRPATATVRVGDRVEHGIHLLQLWMIRPMG
                     GEEVLVVYPQDGYWRIRPKTPSGLDTTVFGSSFLIGPVEDEGRPVVRLEELAFDPETK
                     TFTLTFERGGSATVQIVETDQNRHALHVVFDKPIEGRPFAAMRSMYVTEFNNDAARIA
                     LREPGAKGWREEPIMSFDRAMATDIWLGRLTPSRHNTSSPDMIFNSFSETTKPRLRRN
                     EPPPEVEPKK"
     gene            complement(4872..5645)
                     /locus_tag="VF226_15670"
     CDS             complement(4872..5645)
                     /locus_tag="VF226_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7075477.1"
                     /translation="MTIADERLTALKRDPRLASLKRSLEIYYGDSARNAAMEALYGRF
                     VGHGDLAFDVGSHVGDRIGAFRRLGARVVAVEPQPDCGYVIRSLYGDDPGVTLVEAAC
                     GREPGRTLLHVNTANPTVTTASADFIAAAEGAGGWEGQVWDRQVEVEVTSLDDLARTF
                     GRPQFVKIDVEGYEAEVLAGLSSALPALSFEFTTIQRDVALRCTDRLLDLGPYRFNAA
                     LGEMHVLQFDEWVGGQQIKTFIESLPHEANSGDIYALRS"
     gene            complement(5642..7447)
                     /gene="mdoH"
                     /locus_tag="VF226_15675"
     CDS             complement(5642..7447)
                     /gene="mdoH"
                     /locus_tag="VF226_15675"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX7075478.1"
                     /translation="MSEHHVLLTPMPALDNRTVLTTPTGTQSIEDLRRRRLVVTVLNV
                     STYLLLMWGISLVLAAGGWTSIDTILFLAFAIGSPWTVLGFWNSVIGLWLLHGVRDPM
                     ERVAPFAKAGDRNTPISIRTAVLMTLRNEDPARAFLRLKTVKQSLDATGWGDQFTYFI
                     LSDTSDPSVAVAEEEAAQSWRRSLPDPERVIYRRRAENTGYKAGNLRDFCERWGRDFE
                     LMLPLDADSLMSGETIVRMVRIMQAHPQLGILQSLVVGMPSASAFARIFQFGMRHGMR
                     SYTMGQAWWAGDCGPFWGHNALVRITPFARQCHLPILPGKPPLGGHVMSHDQVEATLM
                     RRAGYEVRVLPEECGSWEENPPTMLEFMRRDVRWCQGNMQYMKLLDMPGLLPMSRFQL
                     TWAILMFIGIPAWTLMIALLPLVAWEAQSVPDYPASLAAGLYVLFFTMYMSPKIAGLA
                     DAVLTPGGVARYGGRLRFAASAVIELVFSFLLGAVSTIRTTIFMIGLMFGKSVNWGGQ
                     ARDAHGISWRLAWSNLWPQVVFGTLVCGALAAISPATLIWSLPLTAGYLLAVPFGVLT
                     ANPALGRLLKATGICGIPEDFAPPPEIQAVQNWHA"
     gene            7704..8507
                     /locus_tag="VF226_15680"
     CDS             7704..8507
                     /locus_tag="VF226_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEX7075479.1"
                     /translation="MRAPFQRGAFPFPVKYGYCAAGVVEAGPKELRGRTVFVLHPHQD
                     FFVAPASLVAVVPDAIPPKRATLAANMETALNALWDANCGPGDRIVVVGGGVIGLLTA
                     FLAAQMPGTEVTLVDVMPERDPIARQLGVGFAVPSQAPRDADVVFHTSATAEGLSTAI
                     GAAGLEGTIVEMSWYGEGCIPVPLGCAFHSRRLRLISSQVTEVAPTHRPRWTRRRRLE
                     AALSLLQDPCLDALITDEIDFTEAPARLPKVLAANAPGLAPVIRYSTAV"
     gene            8523..8927
                     /locus_tag="VF226_15685"
     CDS             8523..8927
                     /locus_tag="VF226_15685"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX7075480.1"
                     /translation="MYAVEVRGRIMIAHSLPDPIFGPAQKMHGATYVVDVAFFREKLT
                     RHNVVVDIGAALSALDDVLKPLAYQNLDELPQFKGKLTTTEFLCGYIFEAMAKAAREG
                     ALGEDGKSLARIRVTLHETDLARASFEGPVGD"
     gene            8920..9966
                     /locus_tag="VF226_15690"
     CDS             8920..9966
                     /locus_tag="VF226_15690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7075481.1"
                     /translation="MIDVAFAIPGDIATPTGGYAYDREVLARLPEHGIRARHLPLPAG
                     YPDPTPHELAETTRLFAGLSPDTVLIIDGLAFGAMPAEAIAGTRQRIIALVHHPLGYE
                     PGLSEERARQLVELERKALTFARAVLVTSPFTKRLLVDKFAVPERKITIAQPGTEPAE
                     RAHGTGEPLQLLSVGSVTPRKAFPTLVTALAPLAGLDWRLTIAGPLDRDPIAVAELKS
                     AIARLGLEDRIALTGALNRQQIASAYAAADVFVLPSLFEGFGMVLTEAMARGLPIVCT
                     TGGAAAETVPDAAAIKVPPGAVGPLTEALRQVIADRDLRRRLADASWEAAQHLPTWEQ
                     TARRIADLIRRVSA"
     gene            9963..10766
                     /locus_tag="VF226_15695"
     CDS             9963..10766
                     /locus_tag="VF226_15695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7075482.1"
                     /translation="MSGFSPDWLALREPADHAAINAEVRAACVRAFAGRELVKVVDLG
                     CGTGSNLRSLAPALGARQQWLLVDNDPVLLAAAKRSTTGSFGKIDLACTQVDLSSTDW
                     RSLLHDADLVTASALFDLVSAPFIEAMASEIAAMGCAFYTVLTYDGIAAFLPEHPADR
                     PMREAFNRHQQTDKGFGPAAGPAATDVLANAFRRHGYKVRRGKSPWVLDNIRVPLRSE
                     LEKGWAAAVRETGLVPDHTIDNWLAHRQGAPSAVTIIGHEDLLALPEGD"
     gene            complement(10763..11344)
                     /locus_tag="VF226_15700"
     CDS             complement(10763..11344)
                     /locus_tag="VF226_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEX7075483.1"
                     /translation="MNEQIDAKSGAVEAYSAPARFFHWLTAVLVAIMVPVGLTMNYRG
                     NTLNIWDDVTNHLYSTHKLVGFLLLWVVVVRIGYRLWHGAPPPEPTLEPWQRYGSAVV
                     HYSLYALILIMPILGWLGVSLFPALEVFGAFSLPALAEPDQELSKQVLSAHKAVAFLL
                     IALILGHAGMALFHHFVRKDNVLWRMLTSVGRR"
     gene            complement(11341..12051)
                     /locus_tag="VF226_15705"
     CDS             complement(11341..12051)
                     /locus_tag="VF226_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX7075484.1"
                     /translation="MGGRLDATAEDPCGPIRDADPNRPFVVAQLGQSLDGRVATVTGE
                     SRWINRDAALDHLHRLRACVDAVVVGIGTVLADDPMLNVRRVPGTHPARVIIDPNKRL
                     PAGAQCLASDGARRIVIRTSNSGAVAGADVICLEATGGVIDPKAIVEALFEQGLRRLL
                     IEGGARTVSSFIDADVVDRLHILVAPMIIGSGRTGLTLEPISRLSDARRPATRVHLLE
                     DGDVLFDCDLRSDGRRTA"
     gene            12211..14508
                     /locus_tag="VF226_15710"
     CDS             12211..14508
                     /locus_tag="VF226_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075485.1"
                     /translation="MQSAVAQPLDRSLAAIVALMLSSWLAFCWPWLSGAVTIPWDAKA
                     QFQPQVQFLAASIARGELPFWTPYAFAGHPQIADPQSMMFSPPMLLLALIDPAPGNWA
                     VDATVLLSVLFGGFGVVLLCRDLGWHWAAVLVAALAFQFGGVMAWRIQHFGQVLSMVY
                     LPLALFFLRRALERSSPVYGTAAGLFAAFIALGRDQVALLSLYLLAGYVVWHWLTAAS
                     PVAAAKASIKPLACAAVSSLLVAAIPITLTALLAGQSNRPEIDFIGAGRGSLHPALLL
                     TAAIPDLFGAAGEMEVYWGPPSYTWTGTDLFIAQNVGQLYLGAIPLILLVIGICRGVL
                     WSRDTRFFSISALLVLLYALGWYTPVFRLAYDILPGVSLYRRPADALFLVGGLGALLA
                     GYVAHRMLSGTLPQVAAWRRWLEAGVVLLLFALGLVLALNFDHLEAATAPMLRAAASF
                     GIGAVILFAVGKWWPARPMLMTGLLVAFATADLAVNNGPNGATAMPPAELEFLEPDTG
                     NETIRLLQRKVSEASDEVSRPRVEIAGVGFHWPGSTLTHRLEHTLGYNPVRLGLYSKA
                     TGAGDTVGLPDQRKFTPLFPSYRSQLADLLGLRFIATGVPIEKIDKNLASDDLKLIAR
                     TSDAYIYENPRALPRVLFAHEAVEGDFEAMLASGKWPTDDLATTVVLENAPVSPAHRP
                     GKARIVRYENTEVVIEADSPDGGWVVLNDVWHPWWFAEVDGMPVTLKRANVLFRAVEV
                     PSGRHTLRFVFRPLAGAMREILDRM"
     gene            14560..16338
                     /locus_tag="VF226_15715"
     CDS             14560..16338
                     /locus_tag="VF226_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015441 - ABC-type beta-glucan transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015775 - beta-glucan transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan ABC transporter ATP-binding protein/
                     permease"
                     /protein_id="HEX7075486.1"
                     /translation="MKLVSVYSRAIGQLMSERWLAFSLILANIGVGLVALAEPVLFGK
                     VVDGLSQSTAVFPLIMLWAALGLFGITAGVLVAVAADRLAHRQRLAAMSGAFERTLTL
                     PISYLSERGTGATVRTILAGADALFATWLTFMREQLSAMVSILLLIPTAIYMDWRLAS
                     LLAVLAVIYTVLNVMVVHRTSDGQAAVERYYMDVFGRVGDVIGNIPVVQSYGRLTAEA
                     AALRDLMRQLLAAQYPVLTWWGLLNILTRAASTVTMVSIFSVGAFLANRGEISVGEIV
                     SFVAFASLLIGKLDQLSGFVARIFMQAPTLKSFFDLLDTTVAIVERPDAKPLQNITGA
                     VRYENVTFRYGNNHQGVFDLNLEARPGETIALVGPTGSGKTTTLAMLQRLRDPEAGRI
                     LVDGHDIRDITLESLRRGMAVVFQDAGLFNRSIAENIRIGRPTATDAEVEEAARLAEA
                     HAFIMRKPHGYNTLAGERGAALSGGERQRIAIARAILKQAPILILDEATSALDTETEG
                     KIKRALDAVRRGRTTFIIAHRLSTVANADQILVFDQGRIVERGTFKELVHKGGLFARL
                     VAEGGFTEPNEPDQPRAKLLEAEAVS"
     gene            complement(16394..17287)
                     /locus_tag="VF226_15720"
     CDS             complement(16394..17287)
                     /locus_tag="VF226_15720"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7075487.1"
                     /translation="MSAAQDDAEVWFTCANELGEGSVAHPDRNSLIWCDILQRQLYER
                     AFSGGEARVHDLDAMPSATALVDARHILLVTDVDLRLFDLETGLGRPLMPFLDDQPGL
                     RGNDGRVHPGSGAFWFGSMGKNAETEAGAIWWFRGGELRMLFDRITIPNAICFSSDGA
                     CAYFSDSHKQTVWRVEVDPATGLPTNEPAVFCRFTRGDGAPDGAIVDADGILWIAGWG
                     ASALHAWGPDGRQIQKYRLPVSQPTCPAFFGPALNEIVVTSSFEHMSAEARAAEPFAG
                     SVVKLRRNVRGIREPHVKIAA"
     gene            17724..18083
                     /locus_tag="VF226_15725"
     CDS             17724..18083
                     /locus_tag="VF226_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075488.1"
                     /translation="MEAGESTDHFPPSRVLPLRPGSPADARRARNRPLGLEAEGSPSA
                     SQRRAAQRTGADYSQRECEQIYDSGTGMTRQQWSASCARVGQRMDQLREIGRQQIRRP
                     SPTSPSGIGVFGPMQNR"
     gene            complement(18228..18587)
                     /locus_tag="VF226_15730"
     CDS             complement(18228..18587)
                     /locus_tag="VF226_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075489.1"
                     /translation="MKRVRIWVAAALLAGANSAAMAFDVQQGAQAPAASPEANAAPAT
                     EAPLGADLSMTETNSAPSASGTEVRIPGLGKLGVLPKMDFGLELLYGATDPKQPEATP
                     EQSADGVVVRGTVKHRF"
     gene            18814..19311
                     /locus_tag="VF226_15735"
     CDS             18814..19311
                     /locus_tag="VF226_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075490.1"
                     /translation="MNPVPVSVCRALGARYVIAVNLNADMCGRGTVVPFLEAGTQDTL
                     LIEANGVPKKNGRAAMKLLRRQFFGPDETAPGISTVVMDAFNIVQDRIARSRLAGDPP
                     DFMISPRLAGIGPFDFHRAAELIERGTAAAHREVEDIAREIEVRRLFEPRLEEERAGA
                     VVARE"
BASE COUNT         3436 a         6368 c         6158 g         3401 t
ORIGIN      
        1 ttcacgtggc tcgacgacga cggcaccatc ttcaagcacg agcagaaagt aacggtcgcc
       61 tgaccgagct cttcctcgaa cacatacaat gaggtagcgg catgatctcg aggcgcgagc
      121 tcgtgcaggc ggcgctgatc gcatcggcgt cgattggtgg ctccatgggc tccctggccc
      181 gtgccctcgc ccagggcaag ctgagccagg acgcactgtt gggcttcagg gagctcggca
      241 acgtcacgct cgtccacatg gcggatctgc acgcgcagct cgtcccggtc tggttccggg
      301 agccgtcgga gaacttcgga gtgggcgagg caagggggcg cgttccccac cttaccggcg
      361 acgacctcct gaaggagttc gggcttgccc ccggctctcc cgaggcctac gccttcagcg
      421 ccgaggattt cgtagagctt gcccgcaagt acggccgcat gggcggcctc gatcgcatcg
      481 cgacggtcat caaggcgatc cgcgcggagc ggccggatcg cacgctggtc ctcgatggcg
      541 gcgatacgct gaccggaagc tggacgtcgc tgaaaacaca agggcaagac atggtcgacg
      601 ccatgagcct catcgcgccc gatgcgacga cggcccactg ggaattcacg cttggcgaag
      661 accgggtcag ggagatcacg gaaaagctcg agtttccttt attggcgcag aacgtccgcg
      721 ataccgattt cgaggactcg gtattcaaag cctacagcct gttcgaacgg ggcggcataa
      781 atatcgccgt cgtcggccag gcgtttccct tcacgcccat tgccaacccg cgctggatga
      841 tcccgaaatg gactttcggc atccgggatt cggacctgca ggagcagatc gatgctgcgc
      901 gggccgatgg cgccgatctc gtcgttctgc tgtcgcacaa tggcttcgac ctcgaccgca
      961 aactcgccag taagctcgac ggcgtcgacg tcatcctgac gtctcacacg cacgacgcgt
     1021 tgcccgaggt ggtcaaggtc ggccgcacgc tgctcatcgc ctccggctcg cacggcaagt
     1081 tcgtggcgcg gctcgatctc gatgttcggg gaaaggaggt caaagatttc cgtttcaagc
     1141 tgatcccgat tttctccgac gtgatcaagc ctgatccgga aatggctgct ttgatttcaa
     1201 agcaccgtgc cccgttcgcc gaggatctcg ctcgcgtcct cgggcggacg aatggcttgc
     1261 tctaccggcg cgggaccttc gccggcacgc tcgacgatct cttttgcgag gcgatgctgg
     1321 ccgaacggga tgccgagatc gccctctcac cgggcatacg ctggggtgca agcttgctgc
     1381 ccggccagga catcacattc gaggatgtga ccaatgcctg tgccctcacc tacccggcct
     1441 gctaccgcac gacgatgacg ggcgcccgcc tcaaggaggt gctggaggat gtcgcggaca
     1501 acatcttcaa ccccgacccg tattaccagc agggcggcga catggtccgt accggcggac
     1561 tggctttcac gatcgacatc ggcgccagca tgggaagccg catttcggat ctcacgctgc
     1621 tcaggaccgg ggagccgatc gaggcggctc gcgagtacgt ggttgcaggc tgggcgagcg
     1681 tgaacgaggg gaccgaggga ccaccgatct gggacgtggt ctccaggcat gtctctgcac
     1741 ggaaggtcat cgatctgaag ccaaacgcca gcgtcaaagt ggttggggcg tgagcgctgt
     1801 atccacaagg agctgtcaca tgacggagga aacaactgga acaggtttgc ggagcctgcg
     1861 cctcaacagg cgtcagctca tcgcgaccgc tgccgcggcc ggtatcggtg cggccggcgc
     1921 tcgtagggcc tatgcgcaag acgttaccgg ctcgatcgca ggcaatcccg aaaatttgcc
     1981 acccaatgtt cccgaatggt cgaaagtcct gggagacggt gtcgatgcgc gtccctatgg
     2041 catgccctcc aagttcgagg cgcatgtcgt ccgtcgcgat gtgaagtggc tcaccggctc
     2101 gcgcgaaagc tcggtgaatt tcacgccgct tcacgagctt gacggcatca tcacgccgaa
     2161 cggactgtgc ttcgagcgcc atcacggggg cgttgccgag atcaacccgc tcgagcaccg
     2221 cctgatgatc cacgggctcg tagacaagcc gctcatcttc tcgatggagg acatcaagcg
     2281 ctttccgcgc gtgaaccgca tccacttcct cgagtgcgcg gccaactcgg gcatggaatg
     2341 gcgcggagcc cagctcaacg gttgccagta tacacacggc atggtgcaca acgtcatgta
     2401 cactggcgtg ccgctgaagc ttcttctgga ggaggccgga ctcaagccga atgccaagtg
     2461 gctgctgctg gagggtgccg acagcgccgg catgacgcga tcgctgccga tcgagaaggc
     2521 gctcaaagac accctgatcg ccttcaaaat gaatggcgag gcgctgcggg aggagcaggg
     2581 ctatccgctg cgggccgtcg ttcccggctg ggagggcaac ctctgggtca aatggctgcg
     2641 ccgcatcgag gtcggcgacc agccctggca tacccgcgag gagacatcga aatacaccga
     2701 cctgctggcg gacggtctct cgcgccgctt cacgtacttc atggatgcca agtccgtcgt
     2761 gaccaacccc agtccacagg cgccgctgat ccacaagggt tacaacgtgc tttccggcct
     2821 ggcttggtcg ggccgcggca ccatcacccg agtggacgtg tcactcgacg gcggccgcaa
     2881 ctggcgcacc gcgcgcatcg acggccccgt tctcgaggcc tcgctgaccc gcttctacgt
     2941 ggagttcgac tggcagggcg aggagctgct gatccagtcg cgggccatgg attcgacagg
     3001 ctacgtgcaa ccgaccaagg ccgagcttcg caaggtgcgg ggcgtgaact ccatttacca
     3061 caacaacggc atccagacct ggctcgtgcg cgccgatgga gttaccgaga atgtcgaaat
     3121 tacctagtct tttcatcgcg ctgaccttgg ccgcggctgc cgcgtcaccc gcgttcggcg
     3181 gcaaattcgg gctggggcga gaggcgacgc ccgaagagat cgcgggctgg gacatcgacg
     3241 tgcgccctga tggccaggga ttgcccgcgg gcaagggaac cgtggccgaa ggagaagaga
     3301 tcttccaaac ctactgcgcg gtctgtcacg gcgagtttgg cgagggcatc gaccgctggc
     3361 cagccctggc cggcgggcgc gactcgctca agaacgatcg cccggacaag accgtcggct
     3421 cgttctggcc ttatgcaacg acaattttcg actatatccg acgggcgatg ccgttcggta
     3481 acgcccagtc gctgtcgccc gacgagattt atgcgctcac cgcctacatc ctctacctca
     3541 acgacatcac gaaggatccg gaattcgagt tgaatcaggc gaacctcccg tccgtcaaaa
     3601 tgccgaacgc cggcaacttc tatgacgacg accgcgaaac cgcggagaag caattctgga
     3661 acaaggaccc ttgcatgaag gactgcaaga agacggtcga gatcctgggt cgcgcgcgac
     3721 tgctcgacgt gaccccggaa agcaagacat ctcctcgggt cgactgacca agcgcatcgt
     3781 ggaagaaggg cgacgacggc tgcggaagcc gtctgccgcc ccgcagctgc gaggcaggca
     3841 gattgctgcc ttcctacttc ttcggttcga cctcgggcgg tggctcgttg cggcgaaggc
     3901 gcggtttcgt cgtttcggag aagctgttga agatcatgtc gggtgagctg gtattgtgac
     3961 gcgaaggcgt cagccggccg agccagatgt cggtcgccat cgccctgtcg aaggacatga
     4021 tcggctcctc gcgccaacct ttcgcgcccg gctcacgcag ggcgatgcgc gccgcgtcgt
     4081 tgttgaactc ggtgacgtac atcgagcgca tcgcggcgaa cggccgacct tcgatcggct
     4141 tgtcgaaaac gacgtgcagc gcgtgccggt tctgatccgt ttcgacgatc tggaccgttg
     4201 ccgagcctcc ccgctcgaac gtgagcgtaa aggtctttgt ctccgggtcg aacgcgagct
     4261 cctcgaggcg caccactggc cggccctcat cctccaccgg accaatcagg aaggaggagc
     4321 cgaacacggt cgtgtccagg cccgaaggcg ttttcgggcg aatgcgccag tagccgtcct
     4381 gcggatagac gacgaggacc tcctcgccgc ccatcggccg gatcatccag agctgtaaga
     4441 ggtggattcc gtgctcgacg cgatccccca cccgaacagt ggccgtcgcc ggccgccaga
     4501 acgtcgggaa ggtcagcccg accagccaga aatcgatctc ctcgtagatc gtcacccggc
     4561 gcggcggaac cggtgccgca aataccggat cgccggacat gtcgcagtcc gtccagtcgg
     4621 gctcgaagct gtcgcgctgc aggttcgaca tgtagaccgg gtgcgctgcc tcgatccgga
     4681 agctgcgcac ccgaggcgat gtgaacgcga tgctgacgtt gtccttctcg gcacagagca
     4741 ccggctggct gccgttagtg acctcgacag gcaccggctc ctgacggaca gcattgggcg
     4801 gctgggatat tgcggggctg gtggcggttg cgaggattgc ggccaagaca agcgcaaacg
     4861 cccgccggcg gtcaggaacg taatgcatat atgtcgccag agttggcctc gtgtggaagc
     4921 gattcgatga aggtcttgat ctgttggccg ccgacccatt cgtcgaactg cagcacgtgc
     4981 atctcgccca gtgcagcatt gaaccgatac ggccccaggt ccagcagccg atcggtgcag
     5041 cgtagcgcga catcgcgctg aatggtggtg aactcgaacg aaagcgcagg aagagcggag
     5101 ctgaggcccg ccagcacctc ggcttcgtaa ccttcgacgt cgatcttgac gaattgcggc
     5161 cgaccgaacg tgcgggcgag gtcgtcgagg ctggtgacct cgacctcgac ttgccggtcc
     5221 cagacctggc cctcccatcc gcccgcacct tctgccgcgg cgatgaaatc cgccgaggct
     5281 gtcgtgacgg tgggatttgc cgtgttgacg tgcagaaggg tgcgcccggg ctcccgcccg
     5341 caggccgcct ccaccagcgt gacgccggga tcgtcgccgt aaagggatcg gatgacatac
     5401 ccgcagtcgg gctgcggctc gaccgccacg acgcgcgctc cgaggcgccg gaaggccccg
     5461 atgcggtcgc ccacgtgact gccgacatcg aaggcgagat cgccgtgacc gacgaacctg
     5521 ccatagagcg cctccatggc cgcgttccgc gctgaatcgc cgtagtagat ctcgagcgat
     5581 ctcttcaaac tcgcgagccg cggatcgcgc ttcagcgcag tgagacgttc atccgcgatg
     5641 gtcatgcgtg ccaattctgt acagcttgga tttccggcgg cggcgcgaaa tcctcgggaa
     5701 tgccgcaaat accggtcgcc ttgagcaatc gtcccagcgc ggggtttgcc gtcagtacgc
     5761 cgaacggcac cgccagcaga tagccggcgg tgagcggcag gctccagatc aacgtggcag
     5821 gggagattgc cgccagcgcg ccgcacacga gtgtaccgaa gaccacttgc ggccagagat
     5881 tggaccaggc cagccgccac gagatgccgt gagcgtcacg cgcctgcccg ccccaattga
     5941 ccgacttccc gaacatcagc ccgatcatga agatggtcgt gcgaatggtc gagacggcac
     6001 cgagcaggaa cgagaacacg agctcgatca ccgcacttgc cgcaaaccgc agccggcccc
     6061 cgtatcgagc cacgccgccg ggcgtcagca ccgcatcggc cagcccggcg atcttcgggc
     6121 tcatgtacat cgtgaagaag agcacataga ggccggcggc caggctcgcc gggtagtccg
     6181 gaacggattg agcttcccag gccaccagcg gcaacagggc gatcatcagc gtccaggccg
     6241 gaatgccgat gaacatcagt atcgcccagg tgagctggaa ccggctcatc ggcagcaggc
     6301 ccggcatgtc gagcagcttc atgtactgca tgttgccctg gcaccagcgc acatcccgcc
     6361 gcatgaactc cagcatcgtc ggcgggttct cctcccagga gccgcactct tccggcagca
     6421 cgcggacctc gtagccggcc cgccgcatca gtgtcgcttc gacctggtcg tgggacatca
     6481 cgtgcccgcc gagcggcggt tttcccggca ggataggcag atggcattgc cgcgcgaacg
     6541 gcgtgatgcg gacaagggca ttgtgtcccc agaacggccc gcagtcgccg gcccaccatg
     6601 cctgtcccat cgtgtaggag cgcatgccat ggcgcattcc gaactggaat atgcgggcaa
     6661 aggcgctggc agagggcatt ccgaccacga ggctctggag gatgccgagc tggggatggg
     6721 cctgcatgat gcgcaccatc cggacgatgg tctcgcccga catcaggctg tcggcatcga
     6781 gcggcagcat cagctcgaaa tcgcgccccc agcgctcgca gaagtctcgc aggttgcctg
     6841 ccttgtagcc cgtgttttcc gcgcgccgtc gatagatcac ccgctctggg tccggcaggg
     6901 agcggcgcca cgactgggcc gcctcttcct ccgccactgc aacggatgga tcgctcgtgt
     6961 cgctcaatat gaaataggtg aactggtcgc cccagccggt cgcatcgagg ctctgcttta
     7021 ccgtcttgag ccgcaggaag gcgcgggcag gatcctcgtt tcgcagcgtc atcagaacgg
     7081 cggtgcggat cgagatcggc gtattccggt cgccggcctt tgcgaacggc gccacccgtt
     7141 ccatcgggtc ccggacgcca tgaagcaacc aaagcccgat caccgaattc cagaaaccca
     7201 gcaccgtcca gggcgatccg attgcgaacg ccaggaacag aatcgtgtcg atcgaggtcc
     7261 atcctccggc agcgagcacc agagaaatgc cccacatcag aagcagatac gtcgagacgt
     7321 taagaactgt cacgacgagc cgacgtcggc gcaggtcctc gatggactgc gtaccggtcg
     7381 gcgtagtaag cacggtccgg ttgtccaacg ccggcatcgg cgtcaacagc acgtggtgct
     7441 cgctcatggc gcactacctc gctgacagct ctcgtcggcc gtcgactgca gggtctttcg
     7501 aaatgaaaaa tgcggcagca ggaagaaccc gctccgcctc cgccctttgg tatgtcgaca
     7561 aggggaaggt ggagctgcga gccgagccac tgcccgagcc gacccgcgag gaagttcttg
     7621 tccgcacctt gttcagtggg gttagtcgcg gcacggaacg cctcattttg tccggagctg
     7681 tccctcttag cgagggggaa cgcatgcgcg cgccttttca acgcggcgcc tttcccttcc
     7741 ctgtcaaata tggttattgc gcggccggcg ttgtcgaggc cggcccaaaa gagctccgtg
     7801 gtcgcacggt ttttgtgttg cacccgcatc aggatttttt cgttgcgccc gcatctctgg
     7861 tggcagtcgt gccggacgcg atcccgccga aacgcgctac cctcgccgcc aatatggaaa
     7921 cggcgctcaa tgcgctctgg gacgcgaatt gcggaccggg cgaccggatc gtcgtcgtag
     7981 gcggcggcgt catcggattg ctgacagcct ttctcgcggc gcagatgccg ggcacggagg
     8041 tgacgctggt cgatgtcatg ccggagcgcg atccgattgc caggcagctc ggcgtgggct
     8101 tcgccgttcc ttcacaagcc ccgcgggatg cggacgtggt ctttcacacg agcgccacgg
     8161 ccgaagggct gtccacggcg atcggcgcgg ctgggctcga aggcaccatc gtcgagatga
     8221 gctggtacgg cgagggctgc atcccggtgc cgctcggctg cgccttccat agccgtcgtc
     8281 tgagattgat atcgtcacag gtgactgagg tcgccccgac acaccggccg cggtggacgc
     8341 ggcggcgtcg ccttgaagcc gccctgtctc tcctgcaaga cccgtgcctc gatgcgctca
     8401 tcaccgatga aatcgacttt accgaggcgc cagcgcggct gccgaaggtg cttgctgcga
     8461 acgcgcccgg gctggcgcct gtcatccgct attccactgc cgtctgacct gaaaggaatg
     8521 ccatgtacgc cgtcgaggtg cgcggccgca tcatgatcgc ccatagcctt cccgatccga
     8581 tcttcggtcc ggcgcaaaag atgcatggcg ccacttacgt ggtcgacgtc gccttcttcc
     8641 gcgagaagct gacccggcac aatgtcgtgg tcgatatcgg cgcagctctg agcgcgctgg
     8701 acgatgtgct gaagccgctt gcgtaccaaa atctcgacga gctgccccag ttcaagggca
     8761 agctgacgac gacggagttt ctgtgcgggt atattttcga ggccatggcg aaggctgcgc
     8821 gtgagggtgc gttgggagag gatggcaaga gcctcgcccg gattcgcgtc accctgcacg
     8881 agacggatct ggcgcgcgcc tcgttcgagg ggcccgtcgg tgattgatgt ggcgttcgcc
     8941 atacccggcg acatcgcgac gccgacgggc ggctacgcgt acgaccgcga ggtgctggcg
     9001 cggctgccgg agcacggcat ccgcgcccgg catctccctc tgcccgccgg ctatccggat
     9061 ccgactccgc acgagctggc ggagaccacg cgcctgttcg cgggtctttc acctgacacc
     9121 gttctcatta tcgacgggct cgcgttcggc gccatgccgg cagaagcgat cgccggcacc
     9181 cggcagagga tcatcgcgct cgtccatcat ccgctgggct atgagcctgg cctttcagag
     9241 gagcgggcac gccagctcgt tgagctggag cggaaggcgc tcaccttcgc aagggccgtt
     9301 ctcgtcacga gcccattcac gaagcggctg ctggtcgaca aattcgctgt tccagagagg
     9361 aaaatcacaa tcgcccagcc ggggactgag ccagccgagc gggcgcacgg gaccggcgag
     9421 ccgctccagc tgctgagcgt cggctcggtc acgccgagaa aggcttttcc aacacttgtc
     9481 acggcgctgg cgccgctggc gggcctcgac tggcgcctga cgatcgccgg gcctttggat
     9541 cgcgatccaa ttgcagtggc agaactgaaa tccgccatcg cacggctcgg cctcgaagat
     9601 cgcatcgcgc tgacaggcgc cctcaatcgc cagcagatcg catcggccta cgcggcagcc
     9661 gatgtcttcg tgctgccgag cctgttcgaa gggttcggca tggttttgac ggaggcgatg
     9721 gcacgcggcc tgccgatcgt ctgcaccacc gggggcgctg cggccgagac cgtgcccgat
     9781 gccgcggcca tcaaggtgcc gccgggtgcc gtgggcccat tgacggaggc cttacgacag
     9841 gtgatcgctg accgtgatct gcggcgccga ttggccgatg cgtcatggga ggccgctcag
     9901 caccttccaa catgggagca aactgcacga cgtatcgcgg atttgatccg aagggtctcg
     9961 gcatgagcgg attttcgccg gactggctcg cccttcgcga acccgccgat cacgccgcga
    10021 tcaacgccga ggtgcgcgcc gcttgcgtac gagcgtttgc gggccgggaa ctggtcaaag
    10081 tcgtcgactt ggggtgtgga acgggctcca atttgcgcag cctggcgcca gcgctcgggg
    10141 cgcgccagca atggctgctc gtggacaacg accccgttct gctcgccgcc gcgaaaagga
    10201 gcacgaccgg ttcattcggc aagatcgatc tggcctgcac gcaggtagac ctctccagta
    10261 ccgactggcg atctcttctc cacgatgcag acctcgtcac cgcctcggcc ctgttcgacc
    10321 tggtctcagc gccgttcatc gaagcgatgg cgtccgaaat cgctgctatg ggttgtgcct
    10381 tctacactgt gctgacctac gacggcatcg ccgccttcct gccggagcac cctgccgatc
    10441 ggcccatgcg ggaagcgttc aacaggcacc agcagaccga caaagggttc ggcccggcag
    10501 ccggaccggc ggcgacggac gtcctggcca atgccttccg ccggcacgga tacaaggtcc
    10561 gaaggggcaa aagtccgtgg gtgctcgaca acatccgcgt gcccttgcgc agcgagttgg
    10621 agaagggctg ggccgcagcc gtccgcgaaa ccggcctcgt gccggatcac accatcgaca
    10681 actggctggc gcaccggcaa ggcgcgccat ctgccgtgac gatcatcggt cacgaggacc
    10741 tgctcgccct gccggaaggc gattagcggc ggccgacgct cgtcagcatc cgccagagga
    10801 cgttgtcctt tcggacgaaa tgatgaaaca gcgccattcc cgcgtgaccg agaatgagtg
    10861 cgatcagcag gaaggcgacc gccttgtggg ccgaaagcac ttgctttgag agttcctgat
    10921 cgggctcggc caacgccggc agcgagaacg ccccgaaaac ttcgagcgct ggaaagagcg
    10981 aaacgccaag ccagccgagg atcggcatga tcaggatgag cgcataaaga gaataatgca
    11041 ccactgccga accgtagcgc tgccacggtt ccaacgtcgg ctcagggggc ggcgcgccgt
    11101 gccagaggcg gtacccgatg cggaccacca ccacccacag cagcaggaag ccgacgagct
    11161 tgtgcgtgct gtaaaggtgg ttggtgacgt catcccagat gttgagcgta ttgccgcgat
    11221 agttcatcgt gaggccgacg ggaaccatga tcgcgacgag caccgcggtg agccagtgaa
    11281 agaagcgcgc gggcgcgctg taggcctcca ccgccccgct cttggcatca atctgctcgt
    11341 tcatgctgtc ctcctaccgt cggatcgcag gtcgcagtcg aacaggacgt caccgtcctc
    11401 gagcaggtga acccgggttg cggggcgccg cgcatcgctc aaccggctga tgggctcgag
    11461 tgtaaggccc gtccggcccg acccgatgat cataggcgcg acgaggatgt ggaggcggtc
    11521 cacgacgtcg gcatcgatga agctcgagac cgtgcgcgct ccgccctcga tcagcagccg
    11581 cctcagcccc tgctcgaaca acgcctcgac aatggccttc ggatcgatga ccccacccgt
    11641 cgcctccagg cagattacgt cggccccggc gacggcacct gagttgctgg tccggatgac
    11701 aattcgccgc gctccgtccg acgccaggca ctgcgcgccg gccggcagcc gcttgttcgg
    11761 gtcgatgatg acgcgcgccg gatgcgttcc ggggacccgg cggacgttga gcatcggatc
    11821 gtcggccagc acggtgccga tgccgacgac cacggcgtcg acgcaagccc tcagccgatg
    11881 caggtggtcg agcgcggcgt cccggttgat ccaccggctt tcgccggtga cggtggcgac
    11941 gcggccgtcc agggactgcc cgagctgggc tacgacgaac gggcggttcg gatcggcatc
    12001 gcgaatggga ccgcaagggt cctcggccgt cgcatctagc cttccgccca tgatcatcct
    12061 ggtctggctc tccgcccgga tccgcgccaa cgtcccattc gtttccgctc gaggcaactc
    12121 tgacgcccgg cactagcgcg ccataatagc atgcaacggt tggagatcag aggatcggag
    12181 catggacctg tgagcgcgtc ggagagggtg atgcaatcgg ccgtggcgca acccctcgac
    12241 cgttcgctgg ctgcgatcgt tgccctcatg ctgtcgagct ggctggcctt ttgttggcct
    12301 tggctgtcgg gagcggtgac gatcccctgg gatgccaagg cacaattcca gccgcaggtc
    12361 cagttcctgg ccgcaagcat tgcccgcggc gagctccctt tctggacgcc ctacgcattc
    12421 gcgggacacc cccagatcgc cgatccgcaa tcgatgatgt tctcgccgcc gatgctgctt
    12481 ctggcgctga tcgatccggc gccgggcaac tgggcagtgg acgcaacggt gctgctctcc
    12541 gtactttttg gcggcttcgg cgtggtcctg ctctgccgtg atctcggctg gcattgggca
    12601 gcggtactcg tcgctgcgct cgcttttcag ttcggcggtg tgatggcctg gcgcatccag
    12661 cacttcggcc aggtcctgag catggtctac ctgccattag cgctgttctt cctgcggcgc
    12721 gcgcttgagc gctcgagccc tgtctacggc accgccgcag gtctcttcgc cgctttcatc
    12781 gcgcttggcc gcgatcaggt ggcgctcctc tctttatacc tgttggccgg atacgtggtt
    12841 tggcactggc tcacggcagc atcgccggtc gctgccgcga aggccagcat caagccgctc
    12901 gcctgcgccg ccgtttcctc gctgctcgtc gcggccattc cgatcacact caccgcgctg
    12961 ctggccggcc agtccaaccg gcccgagatc gatttcatcg gagcaggacg cggctcgctt
    13021 cacccggccc tgctcctcac agccgccata ccggacctgt tcggcgccgc cggagagatg
    13081 gaggtctact gggggccccc aagctacacc tggacgggca cggacctgtt catcgcgcag
    13141 aacgtcgggc aactctacct cggcgcgatc ccactgatcc tgctcgtgat cggaatttgc
    13201 cgcggcgtgc tgtggtctcg cgatacccgc ttcttcagca tctcggcgct tctcgttctg
    13261 ctctacgcgc tcggatggta cacgccggtt ttccgcctcg cctacgacat cctccccggg
    13321 gtttcgctct accgccgtcc cgcggatgcg ctttttctcg tcggcggtct cggcgccctg
    13381 ctggcaggct acgtcgcaca ccggatgctc agcggaacgc tgccgcaagt cgcagcctgg
    13441 cgccggtggc tggaagccgg ggtcgttctc ctgcttttcg ctctcggcct ggtgcttgcc
    13501 cttaacttcg accacctcga agccgcgacc gctccaatgc tgcgggccgc agcatctttc
    13561 gggatcgggg ctgtgatcct cttcgccgtc ggcaaatggt ggcccgcgcg gccgatgctc
    13621 atgacaggct tgctggtggc ctttgcgacc gcggatctcg ccgtcaacaa cggaccgaac
    13681 ggcgcgaccg caatgccgcc cgccgagctc gaattcctgg agccggacac ggggaacgag
    13741 acgatccgcc tgctgcagcg aaaggtgagc gaggcctccg acgaagtttc ccgtccgcgc
    13801 gtggagatcg cgggggtcgg cttccattgg cccggctcga ccctgacgca ccgtctcgaa
    13861 cacacgctgg gttacaaccc tgtccgcctc gggctctact caaaggccac gggagccggg
    13921 gatacggtcg gcctgcccga ccagcggaaa ttcacgccgc tttttccctc ataccgctcg
    13981 cagctcgctg atttgcttgg actccggttc atcgcgacag gcgtgccgat cgaaaagatc
    14041 gacaagaact tggcatcgga tgacctgaag ctcattgcgc gcacatcgga cgcatacatt
    14101 tacgagaacc cgcgggccct gccgcgagtt cttttcgctc acgaggcagt agaaggcgat
    14161 ttcgaagcga tgctcgcgag cggaaaatgg ccaacggacg atctagcgac gacagtcgtg
    14221 ctggagaacg ctccagtctc tcccgcgcac cgacccggca aggcccgaat cgtccggtac
    14281 gagaacaccg aggtcgtcat agaagccgac agccccgatg gcggctgggt cgtgctcaac
    14341 gatgtctggc acccctggtg gttcgccgag gtcgacggga tgcctgtgac attgaagcga
    14401 gccaacgtcc tgtttcgcgc agtggaggtg ccatcaggcc gccatacctt gagatttgtg
    14461 ttccggccgc tcgccggcgc gatgcgcgag atcctggacc ggatgtgacg caagggcccg
    14521 agcacggatg tgtgctggcc atgatcacga cctgatgcca tgaagctcgt ctcggtttat
    14581 tcgcgcgcca tcggccagct catgtctgag cgctggctcg ccttctcgct cattctcgcc
    14641 aatatcggtg tcggccttgt cgcgctcgcc gagcctgtcc tgttcggcaa ggtggtcgac
    14701 gggctgtccc aaagcaccgc ggtgtttcca ctgatcatgc tctgggccgc gctcggtctc
    14761 ttcggcatta ctgcgggcgt gctcgtcgcc gttgcagccg accgcttggc tcaccggcag
    14821 cgccttgccg ccatgagcgg ggccttcgag cgtacactga cactgccgat cagctacctg
    14881 tccgagcgcg ggactggggc cacggtgcgg accatccttg caggtgcgga tgccctgttt
    14941 gccacttggc tgactttcat gcgcgagcag ctatcggcca tggtcagcat cctgctgctc
    15001 atcccgacag ccatctacat ggactggcgg cttgccagcc tgctcgccgt gctggcggtc
    15061 atctacacgg tgttgaacgt gatggtggtc caccgcacct cggacgggca ggccgctgtc
    15121 gagcgctact atatggacgt ttttggccgg gtcggagacg tcatcggcaa cattcccgtc
    15181 gtgcagagct acggtcgcct cacggccgaa gctgccgccc tgcgcgatct catgcgacag
    15241 cttcttgcgg cccagtaccc cgtgctgacg tggtggggcc tgctcaacat tctcacacgg
    15301 gccgcctcga cggtcaccat ggtctcgatc ttttccgtag gcgcatttct ggcgaaccgc
    15361 ggtgaaatct cggtcggcga gatcgtcagc ttcgtcgcat tcgccagcct gctgatcgga
    15421 aagctcgatc agctctcggg cttcgtggca cgcatcttca tgcaggcgcc gacgctcaaa
    15481 tcgttcttcg acctccttga cacgaccgtc gccatcgtcg agcggcccga cgccaagccc
    15541 cttcagaaca tcaccggagc ggtacgctac gagaacgtca ccttccgata cggcaacaac
    15601 catcagggcg tcttcgacct gaacctggag gcgcggccgg gcgagaccat cgctctcgtg
    15661 gggcccaccg gctccggcaa gacgacgacg cttgcgatgc tgcagcgcct gcgggatccc
    15721 gaggcaggcc gcatactggt cgatgggcac gacatccgcg acatcacgct cgagtcgctg
    15781 cgccggggga tggccgtcgt cttccaggat gcgggcctgt tcaatcgcag catcgccgag
    15841 aacatccgca tcggccgccc gactgcgacg gatgccgagg tcgaggaggc ggcgcggctc
    15901 gcagaggccc atgcgttcat catgcgcaaa ccgcacggct acaacactct cgctggcgag
    15961 cggggcgctg ccttgtccgg cggcgagcgc cagcgcattg cgatcgcgcg ggccatcctg
    16021 aagcaggcgc cgattttgat cctcgacgag gcgaccagcg cgctcgacac ggagacggaa
    16081 ggcaagatca agcgcgctct cgatgcggtt cgtcgcggac gcacgacctt catcattgcc
    16141 caccgcttgt cgaccgtcgc caacgccgac cagatcctcg tcttcgatca gggccgcatc
    16201 gtcgagcgcg gtacgttcaa ggagctggtc cacaagggcg ggcttttcgc gcggctcgtc
    16261 gcagaaggcg ggttcacgga gccgaacgag cccgaccagc caagggctaa gcttcttgag
    16321 gccgaggccg tttcctgatt tgcaggcagg cgtgcggggg cgcgaagcgg ccgctgacac
    16381 gtccgcgccc aactcaggcg gcgatcttca cgtggggctc ccgtatcccg cgcacgttac
    16441 ggcgcagctt taccacggat cctgcaaacg gctcagccgc gcgcgcctcg gcgctcatgt
    16501 gctcaaacga cgaggtgacc acgatctcgt tcagtgccgg tccgaaaaat gccgggcatg
    16561 tcggctgact gacgggcagt ctgtactttt gaatctgcct gccgtcaggg ccccacgcat
    16621 ggagagccga ggcgccccag cccgcaatcc agagtatgcc gtcggcgtcc acgatggcgc
    16681 cgtccggcgc gccgtcgcct cgagtgaacc ggcaaaacac cgcaggttcg ttcgtcggca
    16741 gtccggttgc cgggtcgacc tcgacccgcc agaccgtctg tttgtggctg tcggagaaat
    16801 acgcacaagc gccgtcggac gagaagcaga ttgcattggg gatggtgatc ctgtcgaaca
    16861 gcatccgcaa ttcgccacca cggaaccacc agatcgcgcc cgcctccgtc tctgcattct
    16921 tccccatgct gccgaaccag aacgcgccgg aaccagggtg aacgcgcccg tcattgcctc
    16981 gcagaccggg ttgatcgtcg aggaatggca tgagcggccg ccccaatccg gtttcgagat
    17041 cgaacagccg cagatcgacg tcggttacga gcaggatgtg gcgcgcatcg accagcgccg
    17101 ttgccgacgg catcgcatcg aggtcatgca cgcgggcctc accaccggaa aacgcgcgct
    17161 cgtaaagctg ccgttgcaga atatcacacc agatcaggct attgcggtcg ggatgggcga
    17221 cggagccttc gccgagctcg ttcgcacacg tgaaccagac ttcggcatcg tcctgcgcgg
    17281 cgctcatcgt tcatctccct gatcggccgc cccttccacc gttgccgtcg gtgtggaagc
    17341 aggcttttga cttgaaaagg aacaccagcc ggagcggtgc gttgcgtacg caagcttgct
    17401 tcaatgaggt aacgatgcgc acagtgacca tcgccgccac gtgccttgct ttgacgctcg
    17461 gtataggagc ggcccacagc cagacgagtt cacctggcgg agcgccagcc accgggcccg
    17521 cagcgggcgt cagccccaat cgagcaaccg gcaacagccc cgatgccaga tcaaccccct
    17581 tccagcgcgg cctgccttcc aatcctggac tttcctccga ggcaggtggc aggccgctga
    17641 ggcaggatcg cctggatggc accaatctgc ggaatcggat ccggcgtccc gagcggttgt
    17701 cgcgactgcg ccgaggcaat cgcatggagg ctggcgaatc gacggaccac ttcccgccgt
    17761 cccgcgttct gcctttgcgg cccggcagcc ccgccgacgc ccgccgcgcg cgtaaccgcc
    17821 cattgggcct tgaagccgaa ggcagcccct cggcctcgca gcgccgggcg gcccagcgga
    17881 ccggggccga ctacagccag cgggagtgcg agcagattta tgattccggg accggcatga
    17941 cacggcagca atggtccgcg agctgcgccc gcgtcgggca gcgaatggat cagctacggg
    18001 agatcgggcg ccaacagatc aggcggccgt cgccgaccag cccctcgggc attggcgtat
    18061 tcggccccat gcagaaccgg tagaatcgga cgacccagcc cagccagccg gaaaacatgt
    18121 gcccgtccgc agcttcgggc ctgttccgga tcaggccgaa atacgcaaag gcgctccaac
    18181 cacatccccg cttggagaga ggtaaatgga gtgcctgtgg aaaggctcta gaagcggtgc
    18241 ttgacggtcc cgcgcactac gacgccgtcc gccgactgct cgggcgtggc ttccggctgc
    18301 ttcggatccg tggctccgta cagcaactcc aggccaaaat ccattttggg cagcacaccc
    18361 agtttcccga gcccgggaat ccggacctcc gtgccagaag cagacggggc gctattcgtc
    18421 tccgtcatcg acagatcagc gccgagcggc gcttcagtgg ctggggccgc gttggcctcc
    18481 ggcgaagcag ctggcgcctg cgcaccctgc tgcacgtcga acgccatggc tgcactattg
    18541 gctcctgcca gcagcgcggc cgcgacccat atacgaaccc tcttcattca ggactccgcc
    18601 ggcccaaatc atccatgcgt tctgtccggc aacttgagcc gacgcacacc agagtgcaag
    18661 tatgaaccga acaaagttaa ggggttatgt acatttgtcg cgagaggtgt ggtttcgagg
    18721 caataggggg actctgagca ctatgccaga ttgcagggca gcaagcgctg ccgacgcttg
    18781 gctagcttta gccctggggg aagcggggca ctggtgaacc cggtgccggt gtcggtgtgc
    18841 cgggcgctgg gggcgcgtta cgtcatcgcc gtcaatctca acgccgacat gtgcggccgc
    18901 ggcacggtgg tgccgttcct cgaagcgggc acgcaggaca ccctgctgat cgaggccaac
    18961 ggcgtgccga agaagaacgg ccgcgccgca atgaagctgc tgcgccgcca gttcttcggc
    19021 cccgacgaga ccgcgcccgg catctcgacg gtggtgatgg acgccttcaa catcgtgcag
    19081 gaccgcatcg cccgctcgcg gctggccggc gatccgcccg acttcatgat ctctccgcgg
    19141 ctcgccggca tcggcccctt cgacttccac cgcgccgccg agctcatcga gcgcggcacg
    19201 gctgccgccc atcgcgaggt ggaggacatc gcgcgcgaga tcgaggtgcg ccgcctgttc
    19261 gagccgcggc tggaggagga gcgggcgggg gccgtggtgg cgcgggagta ggacgacagc
    19321 cgcaggccca aacaaaaaga gccgccccga agggcggctc cat
//
LOCUS       DASVDU010000244        40695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1007217, whole genome shotgun sequence.
ACCESSION   DASVDU010000244 DASVDU010000000
VERSION     DASVDU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 40695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40695
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007217"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(514..1266)
                     /locus_tag="VF226_15740"
     CDS             complement(514..1266)
                     /locus_tag="VF226_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075491.1"
                     /translation="MNAIDLRGRTAIVTGGSAGIGFAIAKRLLASGAKVAIWGRNAER
                     LKSAAAQLGSSFSLHASTVDVVDETAVREAVQAALDQFGRLDILINNAGILGPRHPVW
                     DYPAKAWREVLDVNLTGAFLCAKAAVPHMRRQKYGRVVNIGSASGKDGNPYVSAYSAA
                     KAGLMALTKALAKETAIDGVLVNCVTPSAAETEIFGELTDQRRAELLSRVPMKRFVDV
                     DEIAALVAWLSSEECSFSTGAMFDITGGRTTY"
     gene            1656..2756
                     /gene="dctP"
                     /locus_tag="VF226_15745"
     CDS             1656..2756
                     /gene="dctP"
                     /locus_tag="VF226_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7075492.1"
                     /translation="MKFAKIVIPTYVAAMVALAGTAHADPSAKPVKLIYAGYITRGGT
                     ATQLDEWFMQEVSRRTNGRVTFEAYYSGSLLNAADLYPGLSRGAADIVTGTPQAYNPQ
                     EYPLSSIVVPYLTDKADAVTYAFNDLYKNNADFRKEFEGKGVEMLWAAAFPETGVWSN
                     KKVGSIADLKGMRIRSALGIAQAFEILGATPVAMTFTEGVDALKRGAIDGMSSAPFDS
                     AVGVGLPKVAAFVSDAGRMGINSVSIASINKRKLDSLDPEARKVILDLAGEMPKKYAE
                     VVDKKIQQSAEKFAAEAGDITVITTPEKEVEAWRAKTADKLKAEWLQLAGKRTKNAEA
                     MFQQYVELVRKYEKASTYKPGLDRFEDLKGKK"
     gene            2789..3331
                     /locus_tag="VF226_15750"
     CDS             2789..3331
                     /locus_tag="VF226_15750"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7075493.1"
                     /translation="MLQKPTIRSCLNAVSRFNTGLGSASGLATMLMMMIIVPDVILRA
                     TISFTIPLATEIGVLLLICKIFLGMPGAQASRANFHVSVITELLGARWQARFKLVTTL
                     ISIGVIGLLAWLTMQEAIYATGIGETSYGIYPFPIWPERIIVAIGFILLTVQLVADAI
                     RILTGVSDDVAPEGSGMGPE"
     gene            3395..4666
                     /locus_tag="VF226_15755"
     CDS             3395..4666
                     /locus_tag="VF226_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7075494.1"
                     /translation="MIAAMLVLLFAGMPIAFALGVAGIFGLAMTRSWQSLEFLLSTFP
                     YSASSNLAYIVLPLFLFMGYMAFAAGVASRAFDAARSWFGAIPGGLPVATVFASSCFA
                     AVSGSSVATAATVAQVAIPEMLRHKYNQRIAAGCVAAAGTLGVLIPPSGILVIYSIAT
                     EVPITDLFVAAFIPGVLTALAYMLLLYVWVQATPAVKEATVLPAAPWRERFQAIGQSW
                     EVIALFALVMGTIYLGLATATEAAAFGAAGALAIVVFRRKNRREIIWRGLVDTGVATS
                     SIFALVIGAGLFGLGLATTQIPQNLAGWVAGFSLEKWQLTLLLISPYLVLGCFIDAIS
                     MLLLTLPVVFPVVVHAGINPILFGILVTKCTEIGAITPPVGLNVFVVKNTVPELDLGE
                     CFKGAFPFVLIELALIGLLVACPQIVTLPLK"
     gene            complement(4817..5722)
                     /locus_tag="VF226_15760"
     CDS             complement(4817..5722)
                     /locus_tag="VF226_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7075495.1"
                     /translation="MKTQKIGDMTIDTVVEMSHKWFDATWLYPTITDEMIQKNKDWIS
                     AMLLDPVNHKVALSFHSFLIRTPNMNIIVDTCNGNHKPRLPKHPWFNMLASNEYLTSL
                     ASHGLQPDDIDVVLCTHLHTDHVGWNTQLVDGKWVPTFPNARYVWAKQEFDYLYEQYK
                     ANPDVPLASGSFNDSVLPVLEAGQVELVDMNHTVDTHLDDKVWMEPATGHTKGHVAIH
                     VEGKDGHAVMTGDIFHHPIIFAEPSLSSLADIDPEQLLKTRLGIMEKCCDTDTLLMTG
                     HFPAPTAGRLVSEKGHIRFRYCEEC"
     gene            complement(5816..6565)
                     /locus_tag="VF226_15765"
     CDS             complement(5816..6565)
                     /locus_tag="VF226_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075496.1"
                     /translation="MLLKDKIAVITGASRERGIGKATAKLFAEHGARVAILDLDEGDA
                     AKAAAEIGPEHIGLRCDVASLDDCKAVAKRVVNWAGRIDILVNNAGLTQRRKVMEVSP
                     ADYDLVTDVVLRGTLYMSQAVIPQMRSQKDGSIICISSLSALQGGGVFGGTHYCAAKA
                     GVLGLMRSMAKELAPEGIRVNGVAPGLILTDFSRGENPDANKHEMAKSFPMGRVGMPR
                     EVGGVCVFLASELSSYVTGATIDVNGGLFIH"
     gene            6812..7627
                     /locus_tag="VF226_15770"
     CDS             6812..7627
                     /locus_tag="VF226_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075497.1"
                     /translation="MSDAAPARSSRLQGKTAVVMGGGSSNPEGGLSNGQAVAIAFARA
                     GAKLVVVDVNKDAAQVTVGLIEKEGGVALAMAADVSKAPDVEAVIRAAVEKHGRIDIL
                     HNNVGIEYMGDVVDTPEEAWDRVHDVNIKSVFLACKYAIPEMEKGGGGAIINISSTAS
                     LKFSGVPYIAYNSSKAALNHMTQIIARQYAHKQIRCNAILPGMMDTPHIRTLYKDLPP
                     EKFKDVMAARDAKCPMGRQGTCWDVANAAVFLASEEASYVNGVLLKVDGASSI"
     gene            7649..8689
                     /locus_tag="VF226_15775"
     CDS             7649..8689
                     /locus_tag="VF226_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7075498.1"
                     /translation="MKRRPTIRDVAKAAGVSVGSVSRVLNGGPYASPDLQSKVARAIS
                     DLDYQPDVVAQSMRLRSTQTIGCMLSEFTNPMYAEIVDAIEERLQREGYLLVCATTRN
                     DAPRETAMISSIRRRRMDGLIVMAGNETFDDVNKALVELEIPTVLIDREVPVNYDAIY
                     VDHRGGAIEATRYLIGLGHKRIALLMPSVQVRPGRERAEGYKAALREAGLPVDPNLIR
                     PQKASTSFAFSEVDRLLQSEDPPTAIITLGTRMLAGVLKAVAARGLSIPDDMSIVSVG
                     DTDIVRCAAPAITAIKWDLAEQGRDAAGLLIEQLNGVKRAGPVHLSLPTELIIRNSCA
                     APRSRTKKRVRG"
     gene            8691..9560
                     /locus_tag="VF226_15780"
     CDS             8691..9560
                     /locus_tag="VF226_15780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075499.1"
                     /translation="MLLALLISGLALGSIYALLGLSLVLVNKATDVVNFAQGEMAMFG
                     AFIALSMMVSMKLPLMLVFVLAFPIGMLMGALIERIMIRPIRNAPHLNLLIVTIGLWF
                     AFNSLAGLIWGYDPYRFPSLLPSEPVDVFGTRVFPASLGVIGIAIVIMAGLYYFFEHT
                     REGTAMRAASMNPRSARLMGIKVTRVAMLSWALSSGLGTVSAMLIAPITFLDQQMMVP
                     VVLKAFAGAILGGFSSLPGAVLGGVILGLSETLLGAYVSNAFKEAFAFLLIIAVLMIK
                     PTGVLGRTARKKV"
     gene            9557..10501
                     /locus_tag="VF226_15785"
     CDS             9557..10501
                     /locus_tag="VF226_15785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075500.1"
                     /translation="MKSVRKDRLLAAVAMGVALVAPAFVGPYGHYVLAIILIYGLVAL
                     SLNIVTGVGGQISIGHAGFWAIGAYTCAILVTKVGLPFLLCVALGGAVAAVFGLIVAL
                     PALRVQGHYLAIATLGLALVVQQVLYEWTSLTGGRGGMFVPRPQILGYEFLDDGPYYY
                     VILAITVFFTWVTVNYRKSLTGLGLGALRMSAIAAQCSGVGRTYHIITAFVLSAFMTG
                     VSGALYGHLIGQLSTDSFTLTVSLSFLTMVVVGGLNSVAGALLGGVFLAYAPELMREL
                     KDAQMIVYGGTLVLFMHFLPGGLASIPERILDLARARR"
     gene            10498..11277
                     /locus_tag="VF226_15790"
     CDS             10498..11277
                     /locus_tag="VF226_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075501.1"
                     /translation="MKPLLSVKQLSLHFAGLKAVQDVSFDVAAGSIVGLIGPNGAGKT
                     TLLNCLSRILNPTCGTIDYDGHDLLKRPVHRLSEIGVTRTFQNLELFGEGTVLENVVV
                     GCFPRFRSNMLSELLSLPGANRARRAAYEEARQLIDELGLTAHVDTVVSGLPYGIQKS
                     VELARALAGKPKLLLLDEPAAGMNPEESRQLGQTIKEMREERGITVVLVEHDMRLVMG
                     ICEKIIVLVQGAKVCEGPPQAVRENSMVIKAYLGEEEAEDA"
     gene            11270..11980
                     /locus_tag="VF226_15795"
     CDS             11270..11980
                     /locus_tag="VF226_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075502.1"
                     /translation="MLEIRDLCAWYGGISALSGVSITVEAGKVLSLLGSNGAGKTTLL
                     TAIMGVMPGRATGSIKFNGEEILGRPAEDVVSMGIALSPEGRQLFPDLTVEENLHMGA
                     YLRRDRGGIAADLERVFTFFPRLRERRRQLASTLSGGEQQMVAIGRALMSSPKLLLLD
                     EPTLGLSPLFVSEILKLVKRINEEGITVLLVEQNARQALRRSSWAYVMEKGRIQMSGA
                     ADKLAKDPHVISAYLGEA"
     gene            12012..13172
                     /locus_tag="VF226_15800"
     CDS             12012..13172
                     /locus_tag="VF226_15800"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075503.1"
                     /translation="MRKLLWSVAGAAVALMTAAGAWANDPGVTDKEIKLGSFLPLQSG
                     LSAGASQYRDGMESYLKYINDQGGINGRKVVWTVENDSYNPQQAVAVAKKLVDRDGVF
                     LIVGTLGTTNTLAAIPFLKQRNVPLLGPLGSHPSINKPKDRIVFPISPLGTLHGTSLA
                     EFAHKDLKAKRIAVFYQDDQYGKELLEGAREFATKNGMEIVNTQSYVPSDVDVSPQAL
                     AISQTKPDAVIMAVIPKQGALFLKEAQKMGWKTNFLAPQLMGDPVAIELAGSAIEGLY
                     VNLYAAIDSMDTPMVKLANQVMAKYAPNTPVGYWSYIGMAGAVIFEQGAKKAGKTLTR
                     ESFMAALESIGKVDAGVIPPVDYTKGAHSGPVTFGYAQVQGGKLKLIHHWND"
     gene            13255..14205
                     /locus_tag="VF226_15805"
     CDS             13255..14205
                     /locus_tag="VF226_15805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7075504.1"
                     /translation="MDINELALTAADHSAPASAGRGSLYLRRKRAHRLADATPIVLLH
                     GSTLPGSAVFDLQLDGLSWMDDLAGTGRDVWCLDLSGYGRSDKPAPRKRADGTEIPVT
                     DTEEALADLAAGIDYILSLSEADQVDLIGWSWGATISAAFCSRPPHAVRSLTLYAPQW
                     LRNTPSPMVTPGVLEKGYRDVDPEKLVERWFKGLKPAIQAEVKARGWMAALIASLQAC
                     DPDAKVIRAPNGTIKDIDQYWMAARPFYDPANITIPTLVIVGTDDQDTPPAQGRTLHA
                     KLGMRTATFVEIPDGTHFMLLEPSRTELFQAVRDFLACQK"
     gene            complement(14243..14836)
                     /locus_tag="VF226_15810"
     CDS             complement(14243..14836)
                     /locus_tag="VF226_15810"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7075505.1"
                     /translation="MEDLPVKTVTPSPEEMKARTARFKDLMPYSKQQENTGIPVAAME
                     RLTANKVYSIMVPEGYKGRSAMAPLKGLPGAVISIAECPPGNGPGLHAHEQTVENFFC
                     LNGKFEVTWGDEGQHSMILEPLDFVSFPPKVSRAFRNISDETARLFVLIQPVSDVQED
                     RVAYAPKLEEELKDSYGAGTVEALKKIGFKFDAGIDA"
     gene            complement(14907..15122)
                     /locus_tag="VF226_15815"
     CDS             complement(14907..15122)
                     /locus_tag="VF226_15815"
                     /inference="COORDINATES: protein motif:HMM:NF018926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEX7075506.1"
                     /translation="MYHEISRMLRASPVPDAEEWAIHDYEGFEGVRVAEYAGIDGVAE
                     TAAFVAEHGRFELAPCKPGDAFSASRR"
     gene            complement(15126..15272)
                     /locus_tag="VF226_15820"
     CDS             complement(15126..15272)
                     /locus_tag="VF226_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075507.1"
                     /translation="MNPKPKEKEMGTNPATAANERPRISMVCLTAYNNGVLHGAWIDA
                     DQEA"
     gene            15795..16061
                     /locus_tag="VF226_15825"
     CDS             15795..16061
                     /locus_tag="VF226_15825"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7075508.1"
                     /translation="MEATIEATLKKHGNSVGLIIPKVLRDALRLGAGQTVTLEQTDGG
                     LLLKPAKQRRFTLDALLAECDPAAPMPEPLREWDSARAVGREEW"
     gene            16055..16405
                     /locus_tag="VF226_15830"
     CDS             16055..16405
                     /locus_tag="VF226_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015397462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ChpB family
                     toxin"
                     /protein_id="HEX7075509.1"
                     /translation="MVRAAGFDRGDIIRVSLNPTVGRELQGGFRPALVLSKRAFNVLG
                     LTLVAPIAQGGNFSRVAGFTVSLAAAGTDTQGVVLVNAVRSMDLQERGGKRIEKAPDF
                     IVDEVLGKLAAIIE"
     gene            complement(16441..17385)
                     /locus_tag="VF226_15835"
     CDS             complement(16441..17385)
                     /locus_tag="VF226_15835"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7075510.1"
                     /translation="MPQRFGMNASRRMDSRQLRYFDAVARERSFHKAAERLRISQPAL
                     SRHIQRLEEEFGTPLLVRHTRGIDLTSEGEILLRHATSILRQFEAARDDVLAHAVEPT
                     GTVSIGAPPTISAILFGDLTGDVARKAPNVSLTLCEGITHYLTDWLETGQLDVAVVGN
                     PPPLHSIATEHLLDEEVYLVGCRSTAFPKENLLPEALVDLPLILATRMETIGSWLEER
                     LGAVGERLNVRYRVESPAIALDLVRRSLGLAVLPRSAIWQKLNDGQIHTALIRQVTLS
                     RYIALPQDRPRTKAMEIVLGSIRERLAHFDGLGAFRSI"
     gene            17792..18316
                     /locus_tag="VF226_15840"
     CDS             17792..18316
                     /locus_tag="VF226_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7075511.1"
                     /translation="MSRIFGAVRQLGYVVHDIDKAMEHWTGQLGIGPFVYFPRVGMSD
                     FKYRGQSYDIELSIALANSGDMQIELIQQRNDVPSIYLDDLKRHGEVLHHTSSWTTDY
                     DADLRRILAAGHRPVQEARIGGNRLAYFECGPGYPSTTMEIYDVSGRAGAFNERIRQA
                     ALAWDGSDPIRSKL"
     gene            18385..19491
                     /locus_tag="VF226_15845"
     CDS             18385..19491
                     /locus_tag="VF226_15845"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF011457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin dehydrogenase
                     dihydrolipoyllysine-residue acetyltransferase subunit"
                     /protein_id="HEX7075512.1"
                     /translation="MSNIKAIRMPKWGLAMEEGTIVGWLKQIGESVKEGEEIVEIETT
                     KITNVLEATESGRLVRIVGDVGEVLPVGSIIAVLADGDASDAEIDEYISAQPAVDVAE
                     KDAAAANALKVETWDVGGRNISVASAGAGQPIVLLHGYSGDFNNWMFTIEDLKSIGRV
                     IAPDLPGHGASAKKMVDGSLGELAGIVAETLKALEVENAWVVGHSLGGAVAQRLALDH
                     PGLVKGLVLVCSAALPGTEVSADFLDDVINAERARDVKAALQWLVADPDLVGREMVDG
                     VLKYLRLDGAREALKVIRDRMLAGDDFSALQKSLDRLPPTLVIAGHNDQIVGRPLQDQ
                     LPGNWDVEWMDAGHLPQLEKAAAFNAILRRHFAK"
     gene            19554..20477
                     /locus_tag="VF226_15850"
     CDS             19554..20477
                     /locus_tag="VF226_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7075513.1"
                     /translation="MATIRHFEERVEKEFANGTIPGFAHVYLGQEASGVGVCFDLTDE
                     DYIGSTHRGHGHCIAKGCEVGGMFLEIMGKDGGICGGKGGSMHIADMTKGMLGANAIV
                     GGSPPIAVGAALTLKYKGTDRVAVAFSGDGASNQGTTLEALNMAVVLKVPVIFAFENN
                     GYGEFTAAEYAVGAPSIAGRAKGFGMPAEVVDGGDFFAVLEAMRRALEHCRGGGGPYT
                     LELNCSRFLGHFVGDPQKYRNQDELKAARSNDPLARFRNSVTGARLLEDAELDAIDAE
                     VRQAVAESAERALAAPQPSLSTLTKDVYLRY"
     gene            20486..21496
                     /locus_tag="VF226_15855"
     CDS             20486..21496
                     /locus_tag="VF226_15855"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7075514.1"
                     /translation="MAKRSYRKAINEALAQEMARDPNVILMGIDVGGGQGGSGKVGSV
                     GGVFGVTYGLYDKFGPSRVIDTPISESAIIGAASGAAVTGLRPVAELMFVDFVGVCLD
                     QIYNQAAKFRYMFGGKAKTPLVIRSMGGAGMRAGAQHSQTLHPLLTMIPGLKVVMPSN
                     PYDAKGLLIKAIRDDDPVFFLESKALYDMEGEVPEDSYEIPFGEANILREGSDVTVVA
                     LGAMVRRAAKAAEALVKEGVKAEVIDPRTTSPLDEDTILESVDKTGRLVVVDESPPRC
                     SLASDIGAMVASRAFKSLKAPIEMVTCPHTPVPFAPNLEDAYLPSPEMIAAAARQAVS
                     FK"
     gene            21505..22665
                     /locus_tag="VF226_15860"
     CDS             21505..22665
                     /locus_tag="VF226_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7075515.1"
                     /translation="MALDRETLDQLLDTVERFVEERLKPAEATVAADDEIPADIVGKM
                     RDLGLFGLSIPEDYGGLGLSMSEEVQVAFRLGGTSPAFRSLIGTNNGIGSQGIVMDGT
                     EQQKQTWLPRLAAGAVIGSFCLTEPDVGSDAGALRTAAVKDGDGYVITGTKRFITNAP
                     EAGLFTVFARTDRDSKDARGVTAFLVEADLKGVGLGRNDHKMGQKGAHTCDVVFDAVR
                     VPASSILGGPAKLGQGFKTAMKVLDRGRLHISAVCVGIAERLIADSLRYAMQRKQFGQ
                     PIAEFQLIQAMLADMKAEAFAARCMVEETARRKDAGASVSTEAACCKMFASEMVGRVA
                     DKAVQIHGGAGYIADYGVERFYRDVRLFRIYEGTTQIQQLVIARNMIRAAAA"
     gene            22714..24369
                     /locus_tag="VF226_15865"
     CDS             22714..24369
                     /locus_tag="VF226_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEX7075516.1"
                     /translation="MDEASANGSGGNSGQGYQLLIKQLLFAPAVRNPDQQIVYRDLKR
                     LTYRQFHARVAQLANALTSLGIGQGDVVAVMDWDSHRYLECFFAIPMIGAVLQTVNIR
                     LSPEQILYTLNHARPKLLLVNTEFLPLLKEVGARVESVKDIILIVDDAVAPIEARQFS
                     KEYESWIAAQPPRFDFPDFSENMPATTFYTTGTTGLPKAVHFTHRQLVLHTLAALATY
                     GTAAVQGRLHQADVYMPITPMFHVHAWGLPYAATTLALKQVYPGRYSPETLLRLKKTE
                     GVTFSHCVPAILHMLLSDRAGQDVDMHGWKIITGGSALPKGLALAAEERGIDIFCGYG
                     MSETGPLSTIAHLAPSFHRDPDKDIDTRITAGRPVILVEVRTVDTSMRDTPRDGVTAG
                     EVVLRSPWLTQGYRGDPEGTRKLWEGGYLHTGDIGILGEDGYLRLVDRLKDVIKTGGE
                     WVSSLDIENVISQHPAVGEVAVIGVKNDRWGERPVALVVLKAAGAELTPDDIKAHMAR
                     YVSSGAISKYGVPDDIRFVESLPKTSVGKLDKKLMRDQFGEGR"
     gene            24419..24955
                     /locus_tag="VF226_15870"
     CDS             24419..24955
                     /locus_tag="VF226_15870"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7075517.1"
                     /translation="MQEYGPKTSVTTMPYPDLEMLPAHLREELARRRNRNVFRMLMHS
                     PEAASGFLAMTDAVRSRNALPPALRELAILRVGRRYGSLYEVHHHERIGREVGLAEAS
                     IRGASDGPFCEGLAPDEILIIRMTDEVLDRHGLTEPSRKAFLERFDAKQLVDFVLTIG
                     HYQQVCNFLNTFGIAVES"
     gene            25180..26214
                     /locus_tag="VF226_15875"
     CDS             25180..26214
                     /locus_tag="VF226_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7075518.1"
                     /translation="MNRRPTIRDVAKAAGTSVGSVSRVLNGGPYASSELHNRVMRAVA
                     KLGYEPDVVAQSMRLKSTKTIACMLADIANPLYAEIVNAAEERLQKEGYVLVLSTTRN
                     DEERELAMISMVRRRRMDGMIVMASDETRADVNTALAEVNVPVVLIDREVPAKYDAVY
                     VDHRGGAIDATRYLLGLGHKRIALLLPSEHVRPGRERAAGYRQALMEAGIAVDPELIR
                     PQKTSVHFAYADIEKLLQGPNPPTAIIVLGTRMLAGVMKTVSTRNLRIPEDLSLISIG
                     DTDLTRFASPPITVVRWDLAELGRAAADLLIEQLNGMKRDRPQHISLPTEFIIRGSCA
                     PPKKPGGKRT"
     gene            26442..27086
                     /locus_tag="VF226_15880"
     CDS             26442..27086
                     /locus_tag="VF226_15880"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7075519.1"
                     /translation="MDPKSNGDDILAKLGRRKIPKSRRLALREPPYDEATAAVVGNAV
                     FQGLSPVNLRMSLANHPKLAAAFQAMSHIVMFQTAVAERDREIAIIRTGALTRSEYEW
                     GMHVSIFGEKCGLTPEQVEDVTLSESWRDLKAGLWNEREQLVVRMVDELHHHSTIADE
                     TWAQLTKHYPNDQVIELIFSSSFYHMAAFYLNSTAVPLEEGAQRFPNGLTQASV"
     gene            complement(27373..28326)
                     /locus_tag="VF226_15885"
     CDS             complement(27373..28326)
                     /locus_tag="VF226_15885"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7075520.1"
                     /translation="MSSSNRPRRASFWNGNWANLRDIEIVCAVILERKTTGAADRLGI
                     SQSAVSRAIAKIEARMKTQLFHRDGGRLSPTAEALRLFERGMAVFGILAELDEPVPDG
                     TAREITILAPPTISHLFLIREIAAFAKAHPDIKVSFDVATIADLASWIAEGRGDVGIS
                     DARIAHSGVAIEPFIETPAICLMRRDHPLARRRRIVPTDLDGVDYVAIHRRHSLRGAL
                     DHIFNKHNVHPHVTIETGSAQFAAELIQRGLGVSVLNPFPLILQRIPNTVHRPFDADL
                     SFQTNFIVPANIPSPVATRMFIDFIKTRRNDVLDTVRMAAA"
     gene            28575..29768
                     /locus_tag="VF226_15890"
     CDS             28575..29768
                     /locus_tag="VF226_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7075521.1"
                     /translation="MSGNQLLSEFAGVAAELVAIRHKIHRHPETAYEEVRTAELIAGL
                     LRRWGLDVADGIGGTGVVGTLRGDRGAGPAIGLRADMDALHIDEVDGRGHRSTIAGKM
                     HACGHDGHIAMLLGAARYLADRRDFRGSVHFIFQPAEEGMAGAKRMIEDGLFDRHPVD
                     RLYGMHNMPGIPAGEFRTRSGAFLAASDTWRVAFRGTGGHGGAGAHAGTDATLAQAGF
                     ILALQTIVSRNVPAIETAVVSVGSVAGGNPDAPNVIPSEVAICGTARSHTAATRELIS
                     RRLTEIAGQQAAVFGCTAEVAYEQIYPPLVTDTRATAISVRAAAALVGGDRVHADAER
                     MTISEDFAFMLERRPGGFILIGNGMDRGGAAQLHTSSYDFNDSIVTVGAAYWVQLVRD
                     ELGSC"
     gene            29856..30644
                     /locus_tag="VF226_15895"
     CDS             29856..30644
                     /locus_tag="VF226_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016735720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7075522.1"
                     /translation="MMDRRSFLFVAMLVASFAASTQQGRADTLADITARGLIRVAVPQ
                     ESPPFGTVGPDMQLQGLDIDVAKLIATKLGVKLAFVPVTLPNRIPYLETNKADIVIAS
                     LGKNPEREKVIDFTDAYAPFFNGVFAPAEVNIARPDDLAGKTLAVARGSIEDLALTKI
                     APPAATIKRFEDNNTTISAFLTGQVDAIVTGNVIAAAILEKNPPRRPELKFIMNKSPC
                     YIGLRKNDDNLKRRINEIIEAAREDGSLNRITRKWLHQDVPADL"
     gene            30714..31385
                     /locus_tag="VF226_15900"
     CDS             30714..31385
                     /locus_tag="VF226_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7075523.1"
                     /translation="MQHHFDYDWLLRYYSLLLHGIAITVELTVVAAAFGTAVGIVCAW
                     VRCFGKNWARLLVTPYIELIRNTPFIIQLFFIFFGLPSIGMRLGELQAANIAVVVNLG
                     AYSCEIIRAGIQATPRGQWEAGSSLAMSHGQVFRHVVLVPALQRIWPALTSQVVIVML
                     GSAVVSQIAAVDITFAANFIQSRSFRAFEAYFVATGIYLGLAVAVREILAAIGRFAFP
                     RRISR"
     gene            31382..32032
                     /locus_tag="VF226_15905"
     CDS             31382..32032
                     /locus_tag="VF226_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7075524.1"
                     /translation="MIDFTTWDVLRNLLLAARWTVLLSLVSLFGGGGLGLTLLFLRIG
                     RDRLGRRMVKWYIELFQGTPLLMQLFLVFFGIGLFGVDVPAWLAAAIALILWTAAFLV
                     EIWRGCVEAIAKGQWEASSSLGMGHLQQMRYVILPQALRIAIPPTVGFAVQVIKGTAL
                     TSIIGFVELSKAGTAISNATFKPFLVYGLVALIYFALCWPLSKFSRKLEGWLHVSH"
     gene            32019..32747
                     /locus_tag="VF226_15910"
     CDS             32019..32747
                     /locus_tag="VF226_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7075525.1"
                     /translation="MSLIEIADVRKRYGALEVLRGVNISVAAGEVIAVIGKSGSGKST
                     LLRCINGLEAIDGGRITVAGEQVTADDARLKALRLKVGMIFQQFNLFPHETAGGNVMM
                     PQRVVRKVSKGEAEAIASRMLERVGLADKFDAYPSELSGGQQQRVAIARALAMQPIAL
                     LCDEITSALDPELVGEVLAVVRELAAQGMTLIMVTHEMKFAYEVCSRVVFMHEGRVHE
                     IGPSETVFTRPATEELQKFLGRAA"
     gene            32915..33844
                     /locus_tag="VF226_15915"
     CDS             32915..33844
                     /locus_tag="VF226_15915"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase"
                     /protein_id="HEX7075526.1"
                     /translation="MDELEGLVAGIVADMAAQTERGNVASYIPQLGRADPLQFGMAVL
                     LGDGRMICGGDADTRFSIQSISKVFTLTLALGKAGDALWRRVGREPSGHPFNSIVQLE
                     YERGKPRNPFINAGALALADVILAGHQPREALGEILRLIRDLADDETIAIDHDVARSE
                     QATGFRNAALANYMRACDMLENPVDYVLGVYFHHCAIAMSCRQLAKAGRYLAFGGRHS
                     DRGGRVISPERARRINALMLTCGHYDGSGEFAFRVGIPGKSGVGGGILGVVPGRASVC
                     VWSPGLDAQGNSRLGRLALERLTRGMGWSVFGV"
     gene            34056..34853
                     /locus_tag="VF226_15920"
     CDS             34056..34853
                     /locus_tag="VF226_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX7075527.1"
                     /translation="MSDAKTEFPAERYLQPSTYIDSESPEVLQLTREALAGLDGASTT
                     ERSIRLFEAVRDGLRYDPYSFAVQADAYRASAIARQESAFCVPKAILLTACLRAAGIP
                     AAVGFADVRNHLNSPKLADLMGTDLFIYHGYVQLWLDGVARKVTPAFNMELCERFGVG
                     PLLFDGRSDALFHEFDADNRRHMEYVNDRGIHLDPPIDELLAAFRTTYPRLVEFTERR
                     IAGPDAAIEDASFPGTAIRKGDAPADRAEGRLSSSRNGLAQKGEYNE"
     gene            34846..35655
                     /locus_tag="VF226_15925"
     CDS             34846..35655
                     /locus_tag="VF226_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7075528.1"
                     /translation="MSDRYAAYDKLTFDYPAKRVLRMTFDRPQTFNSVDAETHTQMTD
                     VWRDIDRDPDINTVIVTGAGKAFSAGGDFGLIESMAGNFNQVMATWKEAKDLVYNIIN
                     CNKPIISAINGPAAGAGLVVGILADISIAGRKAKIVDGHPRLGVAAGDVAAIIWPLLC
                     GMAKAKYYLLTCRAISGEEADRIGLVSMCVEDEVLQDTALEIASELANGSQSAIRWTK
                     YALNNWLRQAGPIFDTSTALEMLGFMGDDVKEGVASHREKRKPNFRTDCPI"
     gene            complement(35705..36667)
                     /locus_tag="VF226_15930"
     CDS             complement(35705..36667)
                     /locus_tag="VF226_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7075529.1"
                     /translation="MTGYPRFVEFREEGPREGFQIEKKIYPIEQRVEFIDLLSDSGLK
                     RIQVGSFVSPRYVPQMADTGELFKRIKRVEGVQYTALWLNDSGFRKALAAKDVHIDPR
                     LLFYASDTFSRKNNNCSAAEMRERQRDWIRLYKENGCTVETAYVMAAFGCNFEGAIPQ
                     DKVLDDFRFVLRLAQEEEIEIPTLVIADTMGWANPEAVRRMIGGLRDIAPEARIGLHI
                     HDTRGLGIANVHAALAMGVDLFETSVAGLGGCPFASHGHARAAGNVCTEDAVFLCHEL
                     GIGTGIDLDKLIAAAQAAERMIGAPLMGRVMHSGGLDRYRNMAD"
     gene            complement(36664..36903)
                     /locus_tag="VF226_15935"
     CDS             complement(36664..36903)
                     /locus_tag="VF226_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEX7075530.1"
                     /translation="MADGVLSSPLTGLIVKVAVHEGDAVAAGDTVAILESMKLEISIK
                     ATASGRAARIAVREGDMVDRGQTIAEIAPLEVQER"
     gene            36844..37545
                     /locus_tag="VF226_15940"
     CDS             36844..37545
                     /locus_tag="VF226_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075531.1"
                     /translation="MDRDLDDQARQWRAQHPIRHRLARRCGERRQDAKAEASAGGFDV
                     DLAGAFRNIGRTRIADDILNTMRAADYAVRETDPFETKPAFSLSAREVSPHVNRIRLL
                     WHRMREPILARFPKPPGRPNDARPYLKQVEEIYVTDAYHSLSIEGYRVSPALIERVRS
                     GNWNPDGNDQDREHRDALAARGYWQTVTAHRRTGGGQTDERGYRPSCCRSCLLRSRDA
                     TAQRRKVSAAMKRLS"
     gene            complement(37596..39800)
                     /locus_tag="VF226_15945"
     CDS             complement(37596..39800)
                     /locus_tag="VF226_15945"
                     /inference="COORDINATES: protein motif:HMM:NF016358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HEX7075532.1"
                     /translation="MTDAPPAPGVVGVEDGGQPGFAGAATRRQAKASPGTLLVASLPA
                     SLIEVGGEGGQEALARLKQTFSRVESSWRPASQEESYEIVRRRLFKEIPGDRHHHKDN
                     TLKQFAKLYRENGNDFPQGCADEDYRRKLEKAYPIHPELFDQLYTSWGSLEKFQRTRG
                     VLRLMAQVIHELWMSNDPSVMIMPGSVAISSARVEPELLHYLDVNWQSIIAGDVDGAN
                     STPYKIDQSAPNLNRYSATRRVARAIFMGSAPTHKEENKGLDDKQINLGVVQPGERPA
                     IFGDALRRLTNQAKFMHSDLGRYWYSMSASLNRIAADRAGQLEEALVLMTIDQELTKY
                     INAISDRGHFDAVQVAPSSSADVPDEPGGVRAVVLGVAHPHTGRDGSEALTEAKDILM
                     QRGSTPRVYRNTLVFLAAESRQLDNLKNAVRSALAWASVVKDDKRLDLKTSDIALAGA
                     KSVEANETLKTRLRECWCYLIYPHQESAQAEVEWASGKVPAQDGLLSRASKKLVSDEG
                     LLPELGPTRLDRDLQRYIWNDKPHLLLKDLWEYLNRYTYLPRLKNQAVLVKAVQAAVS
                     GMLPGPFAYAERWEEGTQKYLGLLIEKAANPHVVIDRDSVIVKPDVAEAHRPRPTAET
                     TESTGAGTGPTATGTGTTTGDQSGQEAGAKQAEKLPTRFIGTVMISSDRPARDMHQIV
                     EAIVQQLTTLPGSDVSLKLEIDAEVSGGLDRAKVRTLMENATTLGFIDKVVK"
     gene            complement(39888..40487)
                     /locus_tag="VF226_15950"
     CDS             complement(39888..40487)
                     /locus_tag="VF226_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7075533.1"
                     /translation="MKIGYARVSTEDQNLALQRQALKRAECERIYEDKGISGAAINRP
                     ALKRALKAAKPGDVLVVWKLDRLGRDLRHLVNLAHELTTKGVGLKVLEGQGAEIDTTT
                     ANGRLIFGIFAALAEFERELISERTRAGMAAAKRDGKHVGRPRKLTDAQIEEARAAVA
                     SGQARKAVAARLGVDAITLRRALSGSGTASGGTEARRER"
BASE COUNT         7535 a        12352 c        12940 g         7868 t
ORIGIN      
        1 gcaagaccat cgatggcctg aaagccttcg cgcctcacga ggtccacgat cgctcgggcg
       61 cggtcatccg ctggcagcat ccccgttccg tccgtttcct gtcccatccg gccgctcgcc
      121 caggaggagt gttcgttcga acactagcgg ccgttctttt cgcttatgtg actttcacat
      181 gaaagtttcg tggcgtcaaa tgaacgcgct tagctggagg ccccagacct ggacggcacc
      241 ggagggactt cccggtcagg gcggccgcgg ccggcatccc tgcctcccgc ggcatcggga
      301 ccgctggcgc gggaggcagg aaagcaaatc ccggcgatca gggccgttcg aagaccatgc
      361 gcggtgttgc cttgagcagt tgatgaaacc cggcgtggtc ggcggcggca cattcgccat
      421 ccatccctgt ccatgcatga tcggctggat gacatccaag gtcggcttgc cgatgaacca
      481 tacgcctgac caatatgtcc gatatcacta tgtctaatat gtcgtacggc cgccggtgat
      541 gtcgaacatg gcgcccgtac tgaaagaaca ttcttcagac gaaagccatg cgacaagagc
      601 tgcgatctcg tcaacgtcca caaagcgttt catcggaacc cgggaaagca actcggcccg
      661 ccgctggtcc gtaagctcgc cgaaaatctc cgtttcggcc gccgatgggg tgacgcagtt
      721 caccagcaca ccgtcaatgg cggtttcctt ggcgagggct ttggtgagtg ccatcaggcc
      781 cgctttggcc gctgaatacg ccgagacata cggatttccg tcctttccgg acgccgagcc
      841 gatattgacc acccgtccgt atttctggcg gcgcatatgg ggcacggccg ctttcgcgca
      901 caggaatgcg ccggtcagat tgacatccag gacttcgcgc caggcctttg ccggataatc
      961 ccagacggga tggcgtggtc caagtatgcc tgcgttgttg atcagaatat cgaggcggcc
     1021 aaattggtcc agcgcagcct gcaccgcctc gcgcaccgct gtctcgtcga cgacatcgac
     1081 tgtcgaagca tgtaggctga agctgcttcc cagctgcgcc gcagcgcttt tcagcctctc
     1141 cgcgttgcgg ccccagatcg cgactttcgc ccctgacgca agcaagcgct tggcgatcgc
     1201 aaaccctatg cctgccgatc ctccggtgac gatggccgtg cggcctcgta aatcgatcgc
     1261 attcacctgg catggtcgaa ccgaaccgaa tgggactgca tcgtcgacag gcatttatgg
     1321 ctcctcaggt ccaggaaatt cccgctgttg cggagagata atagaaacgt ttccagacga
     1381 tttcttcaac tgcgaccctc atggacgatc ctcccggtcc cggggtttat ggggctgccg
     1441 ccggatgccc tcataagcat ctaatttacc tcgggaaaaa gcgagctatg gggcaaagct
     1501 ctttatgccg acgatattcg gtgatcggcc aggggccgga acgggcgtgc cggacaggat
     1561 ggcgatcatc catgttgaaa acgttttcat acttttgtag gttgctcatg gccatggcgc
     1621 aggagctgcc gaattaaagt ggccgggagg aaaacatgaa attcgcaaag atcgttattc
     1681 caacatatgt ggcggccatg gtagcgctgg ccggcaccgc ccatgccgac ccgtcggcga
     1741 aaccggtcaa actgatctac gccggctata ttacccgcgg cgggaccgcc actcagctgg
     1801 acgaatggtt catgcaggag gtctcccgtc gtaccaatgg gcgtgtcacc ttcgaagcat
     1861 actattcagg ctctctgctc aatgcggccg atctctaccc gggcctgagc cgcggggctg
     1921 ccgatatcgt cacgggcacg ccgcaggcct ataacccgca ggaatatccg ctctcgagca
     1981 tcgtcgtgcc gtacctgacc gataaggctg acgccgtcac ctatgccttc aacgacctct
     2041 acaagaacaa cgccgatttc cgaaaggaat tcgaaggcaa gggtgtcgag atgctctggg
     2101 ccgccgcttt tcccgaaacc ggcgtgtggt cgaacaagaa ggtcggcagc atcgctgatt
     2161 tgaaagggat gcggatccgg tcggcattgg gcattgcgca ggcattcgag atcctgggag
     2221 ctacgcccgt ggccatgacc ttcaccgagg gcgttgatgc actgaaacgc ggtgcaatcg
     2281 acgggatgtc gagcgcccca ttcgacagcg cggtcggtgt cggcctgccg aaggtcgccg
     2341 cgtttgtgag cgatgcgggc cgcatgggca tcaattccgt gtcgatcgcc tcgatcaaca
     2401 agcggaagct cgactcgctg gatccggagg cccggaaagt gatcctcgat ctggccggcg
     2461 agatgccgaa aaaatacgcc gaggtcgtcg ataagaagat ccagcaatcg gctgagaaat
     2521 tcgcggcgga ggcgggcgat attacggtca tcacgacgcc ggaaaaggag gtcgaggcct
     2581 ggcgcgcgaa gacggcagac aagctcaaag ccgaatggct tcaactcgcg ggcaagcgga
     2641 ccaagaatgc cgaggcgatg ttccagcagt atgtcgaact ggtcaggaag tacgagaagg
     2701 cgtcgaccta caagcccggc ctggaccgct tcgaggatct gaaggggaag aagtagcccg
     2761 gagcaatcgc ccaacgaaga gaaacgcgat gctgcaaaag ccaacaatcc ggtcatgtct
     2821 caatgcggtg tcgcgtttca atacaggcct cgggtcggca agcggccttg cgaccatgtt
     2881 gatgatgatg atcatcgttc cggacgtcat cctcagggcc accataagct tcaccatccc
     2941 cttggccacc gagatcgggg ttcttctcct gatctgcaag atcttcctcg gaatgcccgg
     3001 agcgcaggcc tctcgcgcaa acttccacgt gtcggtgatt accgaacttc tgggggcgcg
     3061 ctggcaggcg agattcaagc tggtcaccac cctgatatcg atcggggtca tcggcctgct
     3121 ggcatggctg acgatgcagg aagcgatcta tgccaccggc attggtgaga cgtcgtacgg
     3181 gatctatccg ttcccgatct ggccggaacg cattatcgtt gcgatcggct tcatattgct
     3241 gacggtccag ctggtagccg atgccatccg catcctgacg ggagtttccg acgacgtcgc
     3301 gccggaggga tccggcatgg gtccggaata agacgccaca cggcatctga gggaaagcat
     3361 gatgcacctg gacccgtcct taactggggc tgcaatgatt gcagcgatgc tcgttctgct
     3421 gtttgccgga atgccgatcg cgtttgccct gggcgtggcc ggaatattcg ggctggcgat
     3481 gacccgttcc tggcaaagcc tggaatttct gctatcgacc ttcccgtatt cggcctcgtc
     3541 gaacctggct tacatcgtcc tgcccctttt cctgttcatg gggtacatgg cgttcgcggc
     3601 gggagtggcg tcccgggcat tcgatgccgc ccgatcgtgg ttcggggcca taccgggcgg
     3661 attgccggtc gctaccgtat tcgcctcctc gtgcttcgcg gcggtatccg gctccagcgt
     3721 ggccaccgcg gcgactgtcg cccaggtcgc gattccggag atgctccggc ataaatacaa
     3781 ccagagaatc gctgcaggat gcgttgcggc cgctgggacg ctgggcgtgc tgattccgcc
     3841 gagcggcatc ctggtcattt actccatcgc gacagaggtg ccgatcacgg atctcttcgt
     3901 ggcggccttc atccccgggg tgttgacggc tttggcctat atgctcctgt tgtatgtgtg
     3961 ggtgcaggca acgccggcgg tgaaggaggc gacggttctg cctgccgcgc cgtggcgcga
     4021 acgctttcag gcgatcggtc agagctggga ggttattgcg cttttcgccc ttgtcatggg
     4081 cacgatctat ctcgggctgg cgacagcgac cgaagcggct gcgttcggag cggccggagc
     4141 cttggcgatt gtcgtctttc gtcggaagaa ccggcgggaa atcatctggc gcggcctggt
     4201 cgataccgga gtggcgacct cgtcgatatt tgcgcttgtg atcggcgcgg gcctgttcgg
     4261 gctcgggctg gcgacgacgc agatcccgca gaacctggcg ggctgggtgg ccgggttttc
     4321 actggagaaa tggcagctga cgcttctcct gatttcgcct tatctggttc tgggctgctt
     4381 catcgatgcg atttcaatgc tgctcctgac gctgccggtt gtattcccgg tcgtcgtgca
     4441 tgccggtatc aatccgatat tgttcggcat cctggtgacc aaatgcacgg aaatcggcgc
     4501 gatcacgccg ccggtcggtc tcaacgtctt tgtcgtcaag aacacggtgc cagaactcga
     4561 tctcggcgag tgcttcaagg gggcattccc ctttgtcctg atcgaactcg cgctgatcgg
     4621 actgctggtc gcatgtccgc agatcgtcac attgccgctc aaatagctgc gcgcggaggc
     4681 ggttggaatc ccgcctctgc cgacgagcga caaaaagaag gcaggccttg cgacctgcct
     4741 ttgaaatacc gataccggat cgtccgcggc tcgatccatg agcccagaac atatgccgcc
     4801 ccgggaggga agagggtcag cattcctcgc agtatcggaa gcggatatgg cccttctccg
     4861 aaaccagacg gcccgccgtc ggagcgggga agtggcccgt catcagcagt gtgtcggtat
     4921 cgcagcactt ttccataatt ccaagccggg tcttcagaag ctgctccggg tcgatatccg
     4981 caaggctgct caggctgggc tccgcaaaga tgatgggatg gtgaaagatg tcgccggtca
     5041 tgacggcgtg gccgtccttt ccttccacat ggatggcgac atgccccttg gtgtggcccg
     5101 tggccggttc catccagacc ttgtcgtcga gatgggtgtc gaccgtgtgg ttcatgtcaa
     5161 cgagttcgac ctgcccggct tccaacaccg ggaggaccga gtcgttgaac gagccgctgg
     5221 caagcggaac gtctggattg gccttgtatt gttcatacag gtagtcgaat tcctgcttgg
     5281 cccagacata gcgggcgttg gggaatgtcg gaacccactt tccgtcgaca agttgggtgt
     5341 tccatccgac atggtcggta tgaagatgcg tgcacaggac gacatcgatg tcgtccggct
     5401 gaaggccgtg gcttgccagg ctggtcaggt attcattcga cgccagcatg ttgaaccagg
     5461 gatgcttcgg cagccgcggc ttatggttgc cattgcaggt atccacgatg atgttcatgt
     5521 tcggcgtgcg gatcaggaag ctgtggaagc tcaaagccac cttgtgattg accgggtcga
     5581 gcagcattgc gctgatccag tccttgttct tctggatcat ttcgtcggtg atggtcggat
     5641 acagccaagt ggcgtcgaac cacttgtggg acatctccac gacggtatcg atggtcatgt
     5701 cgccgatttt ctgggtcttc atcagtcgtc tccaactctt cggggccggg gcctgattgt
     5761 cggcccggcg ggctgccggc gcagggccgg acggtccatg gctggtcgcc agggctcagt
     5821 ggatgaacag gccgccgttc acatcgatgg tcgctccggt gacgtaggac gacagctctg
     5881 aagccaggaa gacgcagaca cccccgactt cccggggcat gccgacgcgt cccatcggga
     5941 agctcttggc catctcgtgc ttgttggcat ccgggttctc gccgcgcgag aagtcggtca
     6001 ggatgaggcc gggcgccacg ccgttgacgc gtatgccttc gggggcgagc tccttggcca
     6061 tcgagcgcat caggccgagc acgccggcct tggccgcgca gtaatgcgtg ccgccgaaga
     6121 cgccgccgcc ctgcagggct gatagcgacg aaatgcagat gatcgagccg tccttctggc
     6181 tgcgcatttg agggatgacg gcctgcgaca tatacagcgt gccgcgcaga acgacgtccg
     6241 tcaccaggtc ataatcggcc gggctgacct ccatgacctt cctgcgctgg gtcaggccgg
     6301 cgttattgac gaggatatca attcggccgg cccagttgac gacgcgtttg gccacggctt
     6361 tgcagtcgtc gagactggcg acgtcgcagc gcaggccgat atgctcgggg ccgatctcgg
     6421 ccgcagcctt ggcggcgtcg ccctcgtcca gatccagaat cgcgacgcgg gcaccatgct
     6481 cggcgaagag cttcgccgtc gccttgccga tgccccgttc gcgcgaggcg ccggtgatga
     6541 ccgcaatctt atccttcaga agcattttct catcccttgg ttgtcgtttc accagaactc
     6601 cgcatcgttt ggcgatgtct ggttgccggt ccacccggcc catccggccg ccgataatct
     6661 agcggtcgac tagaaacgtt tcaataatat cggtcagaat aatcttataa aaaaccgtga
     6721 aataggcgaa ggacgttgac ggcgcgggct tgtgggtgcc ataagtctgg aaacgatatc
     6781 agaaacgcag accgtttgtg aggaaacgaa gatgtccgac gccgcgcccg ccagatccag
     6841 ccgcctgcag ggcaagaccg ctgtcgtcat gggcggcggt tcctcgaacc ccgaaggcgg
     6901 cctcagcaat ggtcaagccg tcgcgatcgc cttcgcccgt gcgggggcga agctggtggt
     6961 cgtcgacgtc aacaaggacg cggcccaggt aacggtcggc ctcatcgaga aggaaggcgg
     7021 cgttgccttg gcgatggcag ccgatgtcag caaggcgccg gatgtggagg ccgtcatccg
     7081 cgccgccgtc gagaagcacg gccgcatcga tatcctgcat aacaatgtcg gcatcgaata
     7141 tatgggcgac gtggtcgata ccccggagga agcctgggat cgggtccatg acgtcaacat
     7201 caagagcgtc ttcctggcct gcaaatacgc catcccggag atggagaagg ggggcggagg
     7261 cgccatcatc aacatctcgt cgacggcgag cctcaagttc tccggcgtgc cctatatcgc
     7321 ctacaactcc tcgaaggccg cgctgaacca catgacgcag attatcgcgc gtcaatatgc
     7381 ccacaagcag atccgctgca atgcgatcct gcccggtatg atggacacgc cccatatccg
     7441 cacgctctac aaggatctcc cgcccgagaa gttcaaggac gtgatggccg cgcgcgatgc
     7501 gaaatgcccg atggggcggc aggggacgtg ctgggatgtc gccaatgccg cggtgttcct
     7561 cgcgtccgag gaggccagct acgtcaacgg cgtcctgctc aaagtcgacg gcgcctcgtc
     7621 gatctgacga agggcgccag gcatcattgt gaaacgccgt cccaccatac gcgatgtcgc
     7681 gaaggccgcc ggagtctccg tcggcagcgt ctcccgcgtg ctcaatggcg gcccttatgc
     7741 gagcccggac ctgcagagca aggttgcccg tgcgatcagc gacctggatt accagcccga
     7801 tgtcgtggcc cagagcatgc ggctgaggtc gacgcagaca atcgggtgca tgttgtccga
     7861 gttcacgaac ccgatgtatg ccgaaatcgt cgatgccatc gaggaacggc tgcagcgcga
     7921 aggctacctg ctcgtctgcg ccacgacccg gaacgacgct ccccgtgaaa cggcgatgat
     7981 ttcctcgatc cggcgccgcc gtatggacgg tctgattgtc atggcgggca acgagacttt
     8041 cgacgatgtc aacaaggcgc tggtggaact ggagatcccc acggtcctga tcgaccgcga
     8101 ggtcccggtc aactacgacg ccatctatgt cgaccatcgc ggcggcgcca tcgaggcgac
     8161 ccggtacctg atcggcctcg gccacaagcg catcgccctg ctgatgccca gcgtgcaggt
     8221 gcggcccggc cgggaacgtg ccgaaggcta taaggcggcg ctgagagaag ccggcctgcc
     8281 ggtggatccg aacctgatcc ggccgcagaa ggcatccacg agctttgctt tcagcgaagt
     8341 cgaccgcctt cttcagtcgg aggatccgcc gacagccatc atcaccctgg gcacgcggat
     8401 gctggctggc gtcctgaagg ccgtcgccgc ccgcgggctc agcattccgg acgatatgtc
     8461 gattgtcagc gtcggcgata ccgacatcgt ccggtgcgcg gccccggcga tcacggccat
     8521 caagtgggac cttgccgagc agggccgcga tgctgccgga ctgctcatcg aacagctgaa
     8581 cggcgtcaag cgcgccggtc ctgttcacct gtctcttccg acagagctca tcattcgcaa
     8641 ttcgtgcgcg gccccccgct cccgaaccaa gaagcgcgta agagggtagg atgcttctgg
     8701 cactgctgat atcaggtctg gcgctgggga gcatttatgc cctcctcggc ctgtcgctgg
     8761 tcctcgtcaa caaagcgacg gacgtcgtca atttcgccca gggcgaaatg gcgatgttcg
     8821 gggccttcat cgcgctgagc atgatggtgt cgatgaagct gccgctgatg ctggtgttcg
     8881 tcctggcatt tcccatcggc atgctgatgg gggcgcttat cgagcggatc atgatccgcc
     8941 ccatccggaa tgcgccgcac ctgaaccttc tgatcgtcac catcggcctg tggttcgcgt
     9001 tcaacagcct ggcaggcctg atctggggct atgacccata ccgctttcca tcgctgctgc
     9061 cctcggaacc cgtcgatgtg ttcggcacca gggtgtttcc ggccagcctc ggcgtgatcg
     9121 ggattgccat cgtcatcatg gcgggcctct attatttctt cgagcatacc cgcgaaggca
     9181 ccgccatgcg ggcggccagc atgaatccgc gctcggcccg cctgatgggc atcaaggtga
     9241 cccgggtcgc catgctgtcc tgggccctgt ccagcggcct gggcacggtt tcggccatgc
     9301 tgatcgcgcc gatcacgttc ctggatcagc agatgatggt gccggtcgtg ctcaaggcct
     9361 ttgccggcgc catcctgggg ggcttttcca gtcttcccgg cgcggtgctc ggcggcgtca
     9421 tcctcggtct ctccgagacg ctcctcggcg cctatgtgtc gaacgccttc aaggaggctt
     9481 tcgccttcct gctcatcatc gccgttctca tgatcaagcc caccggtgtc ctggggcgca
     9541 ccgcgaggaa gaaagtatga aatctgtcag aaaagaccgg ctgctcgccg cggtcgccat
     9601 gggcgttgcg cttgttgctc ctgcctttgt cgggccgtat ggtcactatg tccttgccat
     9661 catcctgatc tatggcctgg tggcgctgag cctcaatatc gtcaccggtg tcggcggcca
     9721 gatctccatc ggccatgcgg gattctgggc gatcggcgcc tatacatgcg ccatcctcgt
     9781 caccaaggtc ggcctgccat tcctgctctg tgttgcgctt ggcggggcgg tggcggccgt
     9841 attcgggctg atcgtcgccc tgcccgcact tcgtgtccaa ggccattatc tcgccatcgc
     9901 caccctcggc ttggcgctcg tggtccagca ggtcctctat gagtggacca gcctgaccgg
     9961 cggccgcggc ggcatgttcg tgccccggcc gcagatcctg ggctacgaat tcctggatga
    10021 cggcccgtac tactacgtca tcctggccat tacggtcttt ttcacctggg tgacggtcaa
    10081 ctaccggaaa tcgttgaccg gcctcggcct cggcgcgctg cgcatgagcg caatcgccgc
    10141 ccagtgttcc ggtgtcggcc ggacctatca tatcatcacg gccttcgtgc tcagcgcttt
    10201 catgactggt gtatccggcg ccctgtatgg ccatctcatc ggccagctca gcacggattc
    10261 cttcacgctg acggtctcgc tgagtttcct gaccatggtg gttgtgggtg gcctgaattc
    10321 agtggcaggc gcattgctcg gcggtgtctt cctggcctat gcgccggaac tgatgcgcga
    10381 gctcaaggac gcccagatga tcgtctatgg cggcacgctg gtcctgttca tgcatttcct
    10441 gcccggcggg ctggccagca ttccggagcg gatactcgat ctggcgaggg ctcgccgatg
    10501 aagccgcttc tctcggtcaa gcagctctcg ctgcatttcg ccggcctcaa ggcggtccaa
    10561 gatgtcagct tcgatgtggc cgcgggttcg attgtcggcc tcatcggccc caacggggcg
    10621 ggcaagacga ccctgctgaa ctgcctgagc cgcatcctca atccgacctg cggcactatc
    10681 gactatgacg gtcacgacct gctcaagcgg ccggtgcatc ggctgtccga gatcggcgtc
    10741 acgcggacat tccagaatct ggagctgttc ggcgagggca cggtgctgga aaacgtggtg
    10801 gtcggctgct ttccgcgttt ccgcagcaac atgctttccg agcttctgag cctgccgggg
    10861 gccaaccgcg cccgtcgcgc ggcctatgag gaagcccggc agctgatcga tgagctgggt
    10921 ttgaccgccc atgtcgatac agtcgtcagc ggcctgccat acggcatcca gaagagcgtg
    10981 gaattggcgc gagccttggc gggcaagccg aagctgctcc ttctcgacga gcccgccgcc
    11041 ggcatgaatc cggaagaatc ccggcaactc ggacagacga tcaaggagat gcgcgaggag
    11101 cgcggcatca ccgtggtgct cgtggagcac gacatgcgcc ttgtgatggg aatttgcgag
    11161 aagattatcg tgctggtcca gggcgccaag gtctgcgagg gaccgccgca ggccgtcagg
    11221 gagaacagca tggtcatcaa ggcctatctc ggtgaggagg aggccgaaga tgcttgagat
    11281 tcgcgatctc tgtgcatggt atggcggcat ctcggcgctg tccggggtat ccatcactgt
    11341 cgaggccggc aaagtgctgt ccttgctggg ctcgaacggc gccgggaaga cgacgctgtt
    11401 gacggccatc atgggggtga tgcccggccg ggcgaccggc tccattaaat tcaatggcga
    11461 ggagatcctc ggccggccgg ccgaagatgt cgtatcgatg ggcatcgccc tgtcgccgga
    11521 aggccgccag ctgtttcccg acctgaccgt ggaagagaac ctccatatgg gggcgtatct
    11581 ccgccgcgac aggggcggca tcgctgccga tctggagcgc gtattcacct tcttcccgcg
    11641 attgcgcgag cgtcgcaggc agcttgcatc gacgctttcg ggcggcgagc agcagatggt
    11701 ggccatcggc cgcgccttga tgtcgagccc gaagcttctg cttcttgacg agcccacgct
    11761 cgggctgtcg ccgctcttcg tttcggaaat cctgaagctg gtcaagagaa tcaacgaaga
    11821 gggcatcact gttttgctcg tcgagcagaa cgcccggcag gcattgcggc ggtccagctg
    11881 ggcctacgtg atggagaaag ggcgaatcca aatgtcgggc gcggcggaca agctcgcgaa
    11941 ggacccgcat gtaatctccg cgtatctcgg agaggcttag tcaaccaatc gcaatggtag
    12001 ggagtgaaac catgagaaag ttgctttgga gtgttgcagg tgccgcagtg gcactgatga
    12061 cggccgcggg cgcctgggcc aacgacccgg gcgttacgga caaggaaatc aagctgggct
    12121 cgttcctgcc gctccagagc ggcctttcgg ccggtgcgtc ccagtatcgt gacggcatgg
    12181 aatcctacct gaaatacatc aacgatcagg gcggcatcaa cggccgcaag gtggtgtgga
    12241 cggtcgaaaa cgactcctac aatccgcagc aggcggttgc cgtcgcgaag aagctggtcg
    12301 accgcgacgg ggtcttcctg atcgtcggca ccttgggcac caccaatacc ctggccgcca
    12361 tcccgttcct gaagcagcgc aacgttccgc tgctcgggcc gctcggcagt catccgtcca
    12421 tcaacaagcc gaaagaccgc atcgtcttcc cgatttcgcc gcttggcacc ctgcatggca
    12481 cctcgctggc cgaatttgcc cataaggacc tcaaggcgaa gcgcattgcg gtcttctatc
    12541 aggacgacca gtacggcaag gagctgctgg aaggggcgcg cgagttcgcc acgaagaatg
    12601 gcatggagat tgtaaatacc cagtcctatg tgccgagcga tgtcgatgtc agcccgcagg
    12661 cgttggcgat ctcccagacc aagccggacg ccgtgatcat ggcggtcatt cccaagcagg
    12721 gggccttgtt cctgaaggaa gcccagaaga tgggatggaa gaccaacttc ctggccccgc
    12781 agctgatggg cgatccggtc gcgattgaac tggccggctc ggccatcgag ggcctttacg
    12841 tcaacctgta tgccgccatc gattccatgg atacgccgat ggtgaagctg gcgaaccagg
    12901 tgatggccaa atatgccccg aacacccctg tcggctactg gtcgtatatc ggcatggccg
    12961 gcgccgtgat cttcgaacag ggcgccaaga aggccggcaa gaccctgacc cgcgaaagct
    13021 tcatggcggc attggaaagc atcggcaagg tcgatgcggg cgtcatcccc ccggtcgact
    13081 acaccaaagg cgctcattcg ggcccggtaa cgttcggcta cgcccaggtc cagggcggca
    13141 agctgaagct gatccatcac tggaacgact gacgggctgg aacgactggc gggctggaac
    13201 gactgacagg aaggggccgg tccagcccac cccttgccta gtatggagcg gagtatggat
    13261 atcaacgagc tcgcgctgac ggccgcagac cattcggctc ctgcaagcgc ggggcggggc
    13321 tcgctgtacc tgcggaggaa gcgggcgcac cggctcgccg atgccacgcc gattgtgctc
    13381 ctgcatggct cgacccttcc tggaagcgcg gtttttgacc tgcaactcga cggcctctcc
    13441 tggatggacg acctggccgg caccggccgg gatgtctggt gcctggacct gagcggttac
    13501 gggcggtccg acaagcctgc tcccaggaag cgggcggacg gcacggagat ccctgtcacc
    13561 gatacggaag aggccctggc ggacctcgcc gccggtatcg attacatcct ctcgctatcc
    13621 gaagcggacc aggtcgatct catcggttgg tcgtggggcg ccaccatttc agccgccttt
    13681 tgctcgcggc cgccccacgc cgtccgctcc ctcacccttt acgcgcctca gtggctgcgg
    13741 aatacgccgt cgccgatggt aacgcccggc gttttggaga agggatatcg ggacgtcgac
    13801 ccggagaagc tggtcgagcg ctggttcaag ggcctgaagc cggcgatcca ggccgaggtg
    13861 aaggccaggg gctggatggc ggcgctgatc gcatcgcttc aggcttgcga tccggatgcg
    13921 aaggtcatcc gggcgccgaa cggcaccatc aaggacattg atcagtattg gatggcggca
    13981 agaccgttct atgaccccgc caacataacc attccgacgc tggtaatcgt cggcaccgac
    14041 gaccaggata caccgccggc ccaggggcgg acgcttcacg ccaagctggg tatgaggact
    14101 gcgaccttcg ttgagattcc ggacggcacg catttcatgt tgctggagcc gagccgcacc
    14161 gaactcttcc aggccgtgcg cgacttcctg gcatgccaaa agtaacggtc gggcgacgcc
    14221 ccggtcgccg cccggcctgg cttcaggcgt cgatgccggc gtcgaacttg aaaccgatct
    14281 tcttcaaggc ttccacggtg ccggcaccat agctgtcctt gagttcttct tcgagcttgg
    14341 gcgcataggc gacgcggtct tcctggacat ccgatacggg ctggatcagc acgaacaggc
    14401 gggcggtctc atccgagata ttgcggaagg cccggctcac cttggggggg aaagagacga
    14461 aatccagcgg ttccaggatc atgctgtgct ggccttcgtc gccccaggtc acctcgaact
    14521 tgccgttgag gcagaagaaa ttctcgacgg tctgctcatg ggcatgcaga ccgggcccat
    14581 tgcccggcgg gcattcggca atggaaatca cggcgccagg caggccttta aggggagcca
    14641 tggcgctgcg gcccttatac ccctcgggca ccatgatcga ataaaccttg tttgcagtca
    14701 ggcgctccat ggctgcgacc ggaatgccgg tgttttcctg ctgcttgctg tacggcatga
    14761 ggtccttgaa acgcgccgtg cgggctttca tttcctccgg gcttggggtg actgtcttca
    14821 cgggcaaatc ctccatcgat ttgttcgggg ctgcaaaagc gatccattct acggaaacgc
    14881 agcggcaggc ctgtaatacc gagtttctat cggcgcgatg ccgaaaaggc atcgccgggc
    14941 ttgcagggag caagttcgaa cctgccatgc tcggcaacga aggccgccgt ttccgccacg
    15001 ccgtctatcc cggcatattc ggcgacgcga actccttcaa agccttcata atcatggatg
    15061 gcccattcct cggcgtccgg gaccggcgaa gcccggagca tccgggaaat ctcgtgatag
    15121 atcgctcatg cctcctgatc ggcatcaatc catgcgccat gcaggacacc gttattgtag
    15181 gccgtcagac agaccataga gatgcggggc cgctcgtttg ctgcggttgc gggattggtt
    15241 cccatctctt tctccttggg tttggggttc atctgaaccc ttgcccgcgg cgagcatggg
    15301 gtgcaagcgg agcgagcggc ttcgcgggga atcgctgcac ggagcgcaca gcgtggagga
    15361 agctgggcgg aggaagctgg acggaggaag ctgggcggaa gccgctccgc caggggcacg
    15421 gactaagccc ttgcgttagt gatcgtcacc ggatggcgga catgccgggg cggttccgga
    15481 cgcttcgcgg ctagctgtga gctttcaata ggttgtccat ctggtcctga gcgaggaagc
    15541 tgaggttgcg agacttcagt gattcctcgg aggaccggat gaacatccat aagaatgccc
    15601 gtctgacacc gatcggtcga gagcatttgg tgaaggcagt gcagagcggg ctgacgccgg
    15661 aggcaacgcc ctcaattatt cttgggaggt tggccagaga ccgctgtcgt tgctacatcg
    15721 tccggccgca aaggcctgta taacttaatt tgacatttga ccgccagacc catagttata
    15781 caatgtaata ccgtatggag gcgaccatcg aagccacttt gaaaaaacac ggaaacagcg
    15841 ttggcctcat cattcccaag gtgctccgcg atgccctgcg gcttggggca gggcagaccg
    15901 tcacgctgga acagacggac ggcgggttgc tgttgaagcc cgcgaaacaa cgccgcttca
    15961 cgctggacgc tctgctggcg gaatgcgacc cggcggcccc gatgccggaa cctctgcgcg
    16021 aatgggacag cgcccgggct gtcgggcgtg aagaatggta agagcggcgg gtttcgaccg
    16081 gggcgatata atccgggtat ccttgaatcc taccgtgggt cgggaattgc agggcggttt
    16141 ccggcctgcg ctcgtcctgt cgaaacgggc cttcaatgtg cttggcctga cgctggtagc
    16201 cccgatcgca caagggggca atttctcccg cgtggctggg ttcaccgtat cgctggccgc
    16261 agccgggacc gatacgcaag gggtggtgct ggtcaacgcg gtgcggtcga tggacctaca
    16321 ggaacggggg ggcaagagga tcgagaaagc tcccgatttc atcgttgatg aagtgctggg
    16381 aaagctggcc gcgatcattg agtaaggttt gtgatcggga agtttgatgg cgggccggca
    16441 tcagatggac cggaaggcgc caagcccgtc gaagtgggcc agtctctccc tgatgctgcc
    16501 caggacgatc tccatcgcct ttgtccgggg cctgtcttgc ggcagcgcga tatagcggct
    16561 gagggtcacc tgcctgatca gggcggtatg gatctgccca tcgttgagct tttgccagat
    16621 tgccgaccgc ggaaggactg caagcccgag gctccgccgg acaagatcga gcgcgattgc
    16681 cgggctctcg acccggtatc tgacattcaa ccgctcgccg accgcgccca ggcgctcctc
    16741 aagccatgag ccgattgtct ccatgcgcgt cgcgaggatg agtgggaggt ccacaagggc
    16801 ctccggaagc aggttttcct tgggaaaggc cgtggaacgg cagccgacaa gatagacctc
    16861 ttcatccaga aggtgttccg tcgcgatgct gtgaaggggc ggagggttgc caacgaccgc
    16921 gacatcgagc tggccggttt ccagccaatc cgtgaggtaa tgcgtaatgc cctcgcatag
    16981 cgtcaggctg acattcggcg ccttccgggc gacatcgccg gtaaggtcgc cgaacaggat
    17041 cgcgctgatg gtgggcggcg cgccgatgga gacggtgccg gtgggctcga ctgcatgggc
    17101 aagcacgtcg tcgcgagcgg cctcgaactg tcggaggatc gatgttgcat ggcgcagaag
    17161 gatctcgcct tcggacgtca ggtcgatgcc gcgggtgtgg cgaaccagaa gcggggttcc
    17221 gaactcctcc tccagccttt ggatatgacg gctgagggcc ggctgagaga tgcgcagacg
    17281 ttccgcagcc ttgtgaaagc tgcgctcccg agcgacggca tcgaagtatc tgagttgacg
    17341 cgaatccatg cggcgcgagg cattcatgcc gaacctttgc ggcatgacga tttccgcccc
    17401 tccttccgat ctttcttttg gccatcttag tgaaagtcgg gcggagagcc aatgataacg
    17461 tttccatcgc aggcttgatc tgccgcggct tttcgccctg gaggatgggc cgtcttgtgc
    17521 aggcagccgg atcggcggcg tgtcaaacga gatgaataaa agtcatacat gacaattaca
    17581 tagaccatct cggatcggct ccgtgatgct ggcccatagc gtgcggaagc cggaaatgcc
    17641 aataagcatt gatgtaaaaa cgtttctata ttatagaccg ggagcaccca tgccgccggc
    17701 ttgggcaacc ggcagggcag gcggccgaac ccggccggcc atgccgcagc aacagcgcca
    17761 cgccgcaacc acgaagggga tggaaccggg aatgagcagg attttcgggg cggtgcgcca
    17821 gctcggctat gtcgtgcacg acatcgacaa ggcgatggag cattggacgg gccaattggg
    17881 catcggcccc tttgtctatt tcccccgggt cggcatgagc gacttcaagt atcgcggcca
    17941 atcctacgat atcgagctca gcatcgccct ggccaattcc ggcgatatgc agatcgagct
    18001 catacagcag cgcaacgacg tgccctcgat ctacctggac gacctgaaaa gacacggcga
    18061 agtgctgcac catacctcgt cctggaccac cgactacgac gcagacctgc ggcgtatcct
    18121 cgctgcgggc caccggccgg tgcaggaggc caggatcggc gggaaccggc tcgcctactt
    18181 cgaatgcgga ccgggctatc catcgacgac gatggagatc tacgacgtct ccggccgcgc
    18241 cggcgctttc aacgagcgta ttcgccaggc tgccctcgcc tgggacggct cggaccccat
    18301 ccggagcaag ctgtaagtca ttcatcccgc catcgaaggt caggtggcga ccagatttgc
    18361 gcaaacgatc gagcacggac gagcatgtcg aatatcaaag cgatacgaat gccgaaatgg
    18421 ggtctcgcca tggaggaagg caccattgtc ggctggctca agcaaatcgg cgaatcggtc
    18481 aaggagggcg aggagatcgt cgagatcgag accaccaaga ttaccaatgt gctggaagcc
    18541 accgaaagcg gcaggctggt ccggattgtc ggcgatgtgg gcgaggttct gccggtcggc
    18601 tccatcatcg ccgtgctggc cgatggtgat gcctcggatg ccgagatcga cgaatacatc
    18661 agcgcccagc cggcggtgga cgtcgccgag aaggatgcgg ccgccgcaaa tgccctgaag
    18721 gtcgagacct gggatgtggg cggccgcaat atcagcgtgg ctagcgcggg ggccgggcag
    18781 cccattgttc tgctgcatgg ctattctggc gacttcaaca actggatgtt cactatcgag
    18841 gacctgaaat ccatcggcag ggttatcgcg ccggatttgc cgggccatgg cgcctcggcc
    18901 aagaagatgg tcgacggttc gctcggcgag ctggcgggga ttgtggcaga gacgctcaag
    18961 gccctggagg tcgagaatgc ctgggtcgtc ggccactcgc tgggcggtgc tgtcgcccag
    19021 cgcctcgccc tggaccatcc cgggctcgtg aaggggctgg tgctggtctg ctcggcggcc
    19081 ctgccgggca ccgaggtgtc ggccgacttc ctggacgacg tcatcaatgc cgagagggcg
    19141 cgcgatgtga aggcggcctt gcagtggctg gtggccgatc cggatctcgt cggtcgggaa
    19201 atggtcgatg gcgtcctgaa atacctgcgc ctggacggcg ccagggaggc cttgaaggtc
    19261 atccgcgaca ggatgctggc cggcgacgat ttctcggcgc tccagaagtc gctcgacagg
    19321 ctgccgccga cgctcgtcat cgccggccat aacgaccaga tcgtcggcag gccgcttcag
    19381 gatcagctcc ccggcaattg ggatgtcgaa tggatggatg ccggccatct gccgcagctc
    19441 gaaaaggcag ccgccttcaa cgccatcctg cgccggcatt tcgcgaaata ggcactacga
    19501 ctggaagcat catgcagttg agcagagaag atctgaaacg cgcctatcgc cagatggcga
    19561 cgatccgcca ttttgaggag cgcgttgaga aggaattcgc gaacggcacc attcccggct
    19621 tcgctcatgt ctatctcggc caggaggcct cgggtgtcgg cgtatgcttc gacctgaccg
    19681 acgaggatta tatcggctcg acccatcgcg gccacggcca ttgcatcgcc aagggctgcg
    19741 aggtcggcgg catgttcctg gagatcatgg gcaaggatgg cggcatctgc ggcggcaagg
    19801 gcggctcgat gcatatcgcc gacatgacca agggcatgct gggcgccaat gccatcgtcg
    19861 gcggcagtcc gccgatcgcc gtcggcgcag cgctgacctt gaaatacaag ggcactgacc
    19921 gcgtggccgt ggccttctcc ggcgatggcg cctccaacca gggcacgacg ctggaagccc
    19981 tcaatatggc cgtggtcctg aaggtgccgg tcatcttcgc cttcgagaat aacggctatg
    20041 gcgaattcac cgcggcggaa tatgcggtcg gcgccccgtc gatcgccggg cgcgccaagg
    20101 gtttcggcat gcctgccgag gtcgtcgatg gcggcgattt ctttgcggtg ctcgaagcca
    20161 tgcggcgcgc cctggagcat tgccgcggcg gcggcggtcc atacacgctc gagctcaatt
    20221 gctcgcgttt cctcggccat ttcgtgggcg acccccagaa ataccggaac caggacgagt
    20281 tgaaggccgc ccgcagcaac gatccgcttg ctcggtttcg caattcggtg accggcgccc
    20341 ggctgctgga ggatgccgaa ctggatgcga tcgacgccga ggtccggcaa gcggttgccg
    20401 agtccgccga gcgcgccctg gctgccccgc agccgtcgct gtcgaccctg accaaagacg
    20461 tctacctccg ctactgagca tcgccatggc caagagatcc tatcgcaaag caatcaacga
    20521 ggcgctggcc caggaaatgg cgcgcgaccc gaatgtcatc ctgatgggca tcgatgtcgg
    20581 cggcggccag ggcggctccg gcaaggtcgg ctcggtcggc ggcgtcttcg gcgtgaccta
    20641 tggcctgtac gacaagttcg gcccgagccg cgtcatcgat accccgatct cggaaagcgc
    20701 catcatcggt gcggcttcgg gcgccgcggt caccggcctg cgtccggtcg ccgagctgat
    20761 gttcgtcgac ttcgtgggcg tctgcctgga ccagatctac aaccaggcag ccaaattccg
    20821 atacatgttc ggcggcaagg ccaagacccc gctcgtcatt cgcagcatgg ggggcgccgg
    20881 tatgcgcgcg ggcgcccagc atagccagac cctgcatccg ctgctgacca tgattcccgg
    20941 cctgaaggtc gtgatgccgt cgaaccctta cgatgcaaag ggattgctga ttaaggcgat
    21001 ccgcgatgac gacccggtct tctttctgga atccaaggcc ctctacgaca tggaaggcga
    21061 ggtcccggaa gattcctatg aaatcccctt cggcgaggcc aacatcctcc gcgagggcag
    21121 cgacgtcacc gtcgtggctt tgggcgccat ggtgcgtcgt gccgccaagg ccgccgaggc
    21181 cctggtcaag gaaggcgtca aggccgaggt catcgatccc cgcacgacct cgccgctgga
    21241 cgaagacacc atcctggaga gcgtggataa gaccggccgc ctggtggtgg tcgatgaaag
    21301 cccgccgcgc tgctcgctgg cctcggacat cggcgccatg gtggcaagca gggccttcaa
    21361 gtctctcaag gcgccgatcg agatggtcac ctgcccgcac acaccggtgc cattcgcccc
    21421 gaacctggaa gatgcctatc tgcccagccc ggagatgatc gcggctgcgg cgaggcaggc
    21481 cgtttcgttc aaataggcgc accaatggca ttggaccggg aaaccctcga tcagctcctc
    21541 gatacggtcg agcgcttcgt cgaggagcgt ctgaaacctg ctgaagccac ggtcgccgcg
    21601 gacgacgaga tcccggccga tatcgtcggc aagatgcggg atcttggcct gttcggccta
    21661 tccatccccg aggattatgg cgggcttggc ctgagcatgt cggaggaggt ccaggtggct
    21721 ttccggctgg gcggcacatc gccggccttc cgctcgctca tcggcacgaa caatggcatc
    21781 ggctcgcagg gcatcgtcat ggacggcacg gagcagcaga agcagacctg gctgcctcgc
    21841 ctggccgcgg gcgccgtcat cggctccttc tgcctgacag agcccgatgt cggatcggat
    21901 gcgggcgcgc tgcgtactgc ggccgtcaag gatggcgacg ggtatgtcat caccggcacg
    21961 aagcggttca tcaccaacgc gccggaagcc ggattgttca ccgtctttgc gcgcaccgac
    22021 agggacagca aggatgcgcg gggcgtcacc gccttcctgg tcgaggccga cctgaagggt
    22081 gtcggcctgg gccggaacga ccacaagatg ggccagaagg gcgcccatac ctgcgacgtc
    22141 gtcttcgacg ccgtgcgggt tccggccagt agcattctcg gtggtccggc gaagctgggt
    22201 cagggcttca agacggccat gaaggtgctg gaccgcggtc gcctccatat cagcgcggtc
    22261 tgcgtcggca tcgccgagcg cctgattgcg gacagcctgc gctatgcgat gcagcgcaag
    22321 cagttcggtc agccgattgc cgaattccag ctcatccagg cgatgctcgc cgacatgaag
    22381 gcggaggcct ttgccgcccg gtgcatggtc gaggaaaccg cgcgccggaa ggatgccggc
    22441 gccagcgtct ccaccgaggc ggcgtgctgt aaaatgtttg ccagcgaaat ggttggccgg
    22501 gtcgccgaca aggcggtcca gatccacggc ggcgccggct atatcgcgga ttacggcgtg
    22561 gagcggttct accgggatgt gcgcctgttc cgcatctacg agggcacgac acagatccaa
    22621 cagctggtga tcgccaggaa catgataagg gcggcggctg cctgacagtc gaccgccaca
    22681 atagcaatca ggtggacagc aatcaggtgg acaatggacg aggccagcgc aaacggatct
    22741 ggcgggaatt ccggacaggg ttatcagctg ctgatcaagc agctgctttt cgcgcctgcc
    22801 gtccgcaatc ctgaccagca gatcgtctat cgcgacctga agcggctcac ctaccggcag
    22861 ttccacgccc gggttgccca actggcaaat gccctgacct cccttggcat agggcaaggc
    22921 gatgtcgttg ccgtcatgga ctgggacagc caccggtatc tggaatgctt cttcgccatc
    22981 ccgatgattg gcgccgttct gcagacggtc aatatccggc tcagtccgga gcagatcctc
    23041 tataccctca atcatgccag gccgaagctg ctgctggtca ataccgagtt cctgccgctc
    23101 ctgaaggagg tcggtgcgcg cgtggagtcg gtcaaggaca tcatcctgat cgtcgatgac
    23161 gccgttgcgc cgatcgaggc ccggcagttc tccaaggaat atgaaagctg gatcgccgcg
    23221 cagcctcccc gctttgattt ccccgacttc agcgagaaca tgcccgctac gaccttctat
    23281 acgaccggca cgaccggatt gcccaaggcc gtccatttca cgcatcgtca actggtcctg
    23341 cacaccctgg cggcattggc cacatacggc accgccgccg tccaggggcg cctgcatcag
    23401 gctgacgtgt acatgccgat cacccccatg tttcacgtcc acgcctgggg gcttccatac
    23461 gcggcaacga ccctggcgtt gaaacaggtc tacccgggcc ggtattcccc ggagaccctt
    23521 ctccggttga agaagacgga aggggtgacc ttctcgcact gcgtcccggc catccttcac
    23581 atgctgttgt ccgaccgggc cggccaggat gtggacatgc atggctggaa aatcatcacc
    23641 ggtggctcgg cgctcccaaa ggggctggcc cttgccgccg aagagcgcgg catcgatatc
    23701 ttctgcggtt acggaatgtc cgagacggga ccgctgtcga ccatcgcgca tctggctccg
    23761 tcgtttcacc gcgatccgga caaggatatc gatacgcgca tcacggcggg gcggcccgtc
    23821 atcctggtcg aggtccgtac cgtcgacaca tcgatgcggg ataccccaag ggacggcgtc
    23881 accgccggcg aggtcgtctt gcgctcgccc tggctcaccc agggctatcg aggcgatccg
    23941 gagggcacga ggaaactctg ggaaggcgga tatctgcata ccggcgatat cggcatcctt
    24001 ggcgaggacg gctatctgcg cctggtggac cgcttgaagg atgtcatcaa gacgggcggc
    24061 gaatgggtgt cctcgctgga tatcgagaat gtcatctcgc agcatccggc ggtcggcgag
    24121 gttgccgtca tcggcgtgaa gaacgaccga tggggcgagc ggccggtggc gctcgtcgtg
    24181 ctcaaggcgg ctggggcgga gcttacgccc gacgacatca aagcccatat ggcgcggtat
    24241 gtctctagcg gtgccatttc caaatacggc gtgccggatg atatacgctt cgtcgagtcc
    24301 ctgccgaaga cgagtgtcgg aaagctcgac aagaagctga tgcgcgatca gttcggcgaa
    24361 ggccgctgac gcaaggacgc aatgagcggc gccgccgcga ggcgccatgg agggcggaat
    24421 gcaggaatac ggcccgaaga catcggtgac caccatgccc tatccggacc tcgagatgct
    24481 tcccgcacat ctgcgggaag agctggcgcg gcgccggaac cgcaatgtct tccggatgct
    24541 gatgcactcg ccggaggccg catccggctt cctggcgatg acggatgcgg tgcggagcag
    24601 gaatgccctg ccgccagcgt tgcgggagct ggccatcctc cgggtgggac gccgctacgg
    24661 gtccctgtat gaagtccacc accatgagcg gattggccgg gaggtcggat tggccgaggc
    24721 ctccatccgg ggggccagcg atggcccgtt ctgcgagggg ttggcgccgg acgaaatcct
    24781 gatcatcagg atgacggacg aggtcctgga ccggcatggc ctgaccgagc cgagccggaa
    24841 ggcattcctc gagcgcttcg acgcgaagca gctggtcgat ttcgtgctga cgatcggtca
    24901 ctaccagcag gtctgcaatt tcctgaacac tttcggcatc gcggtcgaat cgtgaattcc
    24961 cccgcgaccc cgggccttcg cggcactggg gcgtcctcgc cggcccgtgt tgacgacggc
    25021 gttcgcggaa tgcgataaag cggaaaacgt ttcaaccagg cggcggtgat cgaccataat
    25081 gacacgaaga cgaggcttcg gagacgacct tcggtggtat tctctgcaaa ggcgcggcaa
    25141 tctgggtggc cggctgcaat cggagtgaag gcaagctgaa tgaatcgccg tccgaccatc
    25201 cgcgatgtcg ccaaggcggc aggcacatcc gtcggcagcg tctcccgtgt gttgaacggc
    25261 gggccgtatg ccagttcgga actgcacaac agggtcatgc gggccgtcgc gaaacttggc
    25321 tacgagcccg acgtcgtcgc gcagagcatg cggctgaagt cgaccaagac catcgcatgc
    25381 atgttggcgg atattgccaa tcctctctat gcggaaatcg tcaacgcggc ggaggaacgg
    25441 ttgcagaagg agggctacgt cctcgttctg tcgacgacgc gcaacgacga ggaacgcgaa
    25501 ctcgccatga tctcgatggt ccggcgccgc cgcatggacg ggatgatcgt catggccagc
    25561 gacgagaccc gcgcggacgt gaacaccgca ttggcggagg tgaacgttcc ggtcgtcctc
    25621 atcgatcgcg aggtgcctgc gaaatatgac gccgtttatg tcgatcatcg gggcggcgcg
    25681 atcgacgcaa cgcggtatct gctcggtctc ggccacaaga gaattgcgct gctgctgccg
    25741 agcgagcatg tgcgtcccgg tcgcgaacgt gctgccggtt atcgtcaggc gctgatggaa
    25801 gcgggcattg ccgtcgaccc tgagctgatc cgcccgcaaa agacgtccgt tcacttcgcc
    25861 tatgcggata tcgaaaagtt gctgcagggg cccaatccgc cgacggcgat catcgtgctg
    25921 ggcacgcgca tgctcgccgg cgtgatgaag acggtatcga cgaggaatct gcgcatcccg
    25981 gaagatctct ctctcatcag cattggcgat accgacctga cgcgtttcgc cagcccgccc
    26041 atcacggtgg tccgctggga ccttgccgag ctcggcaggg cggccgctga cctgctgatc
    26101 gagcagttga acggcatgaa gagggacagg ccgcagcata tctcgctgcc gaccgaattc
    26161 atcatccgcg gctcctgcgc cccaccgaag aagccgggcg gcaagcggac ataggcgcag
    26221 gcaggaggcg cggcgggaat cttcagcaat tgggacgtgg cggattgcag cctgccgccc
    26281 cccatgatat cactataaaa acgtttccat taagatgtga atgcggatac atctattgat
    26341 gaagaagtaa taagaatttg tagtagaaac gttattattc caggcgctcc ggccctccca
    26401 cctgaatccg gttcaacacc ccaaagtcag gacccagcag tatggatccc aaatcgaacg
    26461 gcgacgacat cctggcaaaa ttgggccggc ggaaaattcc caagtcgcgg cggctggccc
    26521 tgcgcgagcc gccctatgac gaggcgacag cagcggtggt cgggaacgcc gtcttccagg
    26581 gcctgtcgcc ggtgaacctg cgcatgtcgc ttgccaatca cccgaagctg gcggccgcgt
    26641 tccaggccat gtcgcatatc gtcatgttcc agaccgccgt tgcggagcgc gaccgggaga
    26701 ttgccatcat ccggaccggc gccttgacac gctctgaata cgaatggggc atgcatgtca
    26761 gcatctttgg cgaaaaatgc ggtctgacgc cggaacaggt tgaggatgtg acgctctccg
    26821 agtcctggcg cgacctcaaa gcggggctct ggaacgagcg cgagcaattg gtggtgcgta
    26881 tggtggatga actgcaccac cattccacga ttgccgatga gacatgggcc cagctcacca
    26941 agcattatcc caatgatcag gtgatagagc tgatcttctc gtcgagcttc taccatatgg
    27001 ccgccttcta tctaaacagc accgccgttc cgctcgaaga gggtgctcag cgctttccga
    27061 atggactgac gcaggcatcc gtctaatccg gcggatccgt ttttcggttt aggcgaggat
    27121 ctggcccgca tggccggaac cgattatccg tagcggtcgc tccgcatccg ggtgatgccg
    27181 gccggtccgc cgtttgacgt ccgccgtttg acgtccgccg gaacctccgc cccgctggca
    27241 agcggcccgc ctattcctcg tcgtactcat cacccgagtt ataccgccgg gtccgccagg
    27301 cggtggatgc cgcccttcat ccgatcgacg acggccgccc tgcgcaactg gcctttgcgg
    27361 gcggtcttcc gattaagccg ccgccatcct caccgtatcg agaacgtcat tcctgcgcgt
    27421 cttgatgaag tcgatgaaca tgcgcgtggc cacgggggag gggatattgg ccggaacgat
    27481 gaagttggtt tgaaagctga gatcggcgtc gaaggggcgg tgaacggtgt tgggtatccg
    27541 ctgcaggata agcgggaagg gattgaggac ggataccccg agaccgcgtt gaatcagttc
    27601 ggcggcaaac tgggcgctgc ccgtctcgat cgtgacgtgc ggatgcacat tgtgcttgtt
    27661 gaagatgtgg tccagggcgc ctcgaagcga atgtcggcga tggatggcga cataatcgac
    27721 gccatcgaga tccgtcggca cgatccggcg ccgccttgcc agagggtgat ctcttcgcat
    27781 caggcagatg gcgggggtct cgataaacgg ctcgatggca accccggaat gcgcgatgcg
    27841 cgcatccgag atgccgacat cgccgcgtcc ttcggcaatc cagctggcca gatcggcaat
    27901 tgtggcgaca tcgaaggaca cctttatatc gggatgcgcc ttggcaaagg cggcgatttc
    27961 gcggatgagg aacagatgcg atatcgtcgg cggcgctagg atggtgattt cccgcgccgt
    28021 gccgtccggc accggttcgt cgagctcggc caagatgccg aaaacagcca tacccctctc
    28081 gaacagtctc agcgcttccg ccgtcgggct aaggcgaccg ccatcgcggt ggaagagctg
    28141 cgtcttcatc cgcgcttcga tcttggcaat ggcacgactg accgccgatt gggatatgcc
    28201 aagccggtcg gcggcgccgg tcgtcttccg ttcaaggatg acggcgcaaa cgatttcgat
    28261 gtcgcggagg ttcgcccaat tcccgttcca gaacgaagcg cgtcgcggtc ggttgctgct
    28321 gctcatgggg gtatgactta tcgcataaca attatcatga caatgcaaat gaagtcgatt
    28381 agcagttttc gcgggccgca gtcgcgagcc ggacgcgatc ggaattgtcg aagccaattc
    28441 gcaatgacgt agccggaagg gcagtgcgat gcggatgtcg ggcgtgccgc ggcatgccgg
    28501 ctgcacgccg tcctgcggtg gctggcggcc ggtggccgcg ccttcggtgg gaaagatgcg
    28561 gaaatggggt ggcgatgagc ggtaatcagt tgctctcgga atttgcaggc gtcgcggcgg
    28621 agctggtcgc catcaggcac aagatccacc ggcacccgga aactgcatat gaagaggtgc
    28681 gaacggcgga actgatcgcc ggattgctgc gccggtgggg gctggacgtg gccgatggca
    28741 tcggcggcac cggcgtcgtc ggtacgctgc gtggagaccg gggcgccggt ccggcgatcg
    28801 gcttgcgcgc ggacatggat gccttgcata tcgatgaagt cgacgggcgt gggcatcgct
    28861 cgaccatcgc cggcaaaatg catgcctgcg gccatgatgg acatatcgcc atgttgctcg
    28921 gtgcggcgcg ctatcttgcc gatcgcaggg acttcagggg cagtgttcat ttcatcttcc
    28981 agcctgccga ggagggaatg gccggcgcga agaggatgat cgaagacgga cttttcgacc
    29041 ggcatcccgt cgaccgccta tacgggatgc acaacatgcc gggcataccg gccggcgaat
    29101 tccggacccg aagcggcgcc tttctcgcag ccagcgacac ctggcgcgtc gctttccgcg
    29161 gcacgggcgg acatggcggc gcgggtgcgc atgccggcac cgatgcgaca ttggctcaag
    29221 ccggcttcat cctcgcgctg cagaccatcg tcagccggaa tgtgccggcc atcgagacag
    29281 ccgttgtcag cgtcggatcg gtcgccggcg gcaatccaga tgcaccaaac gtcatcccct
    29341 cggaagtcgc gatatgcgga accgcgcgaa gccataccgc cgcgacgcgg gagctgatca
    29401 gtcggcgttt gaccgagatt gccggacagc aggcggcggt cttcggatgc acggccgagg
    29461 tcgcctatga gcagatctac ccgccgctgg tgaccgacac cagggccacg gccatatcgg
    29521 tgcgcgcggc ggcggcgctg gtcggcggcg acagagttca cgctgacgcg gagcggatga
    29581 cgatttccga ggacttcgcc ttcatgctgg agcgtcgccc gggcgggttc atcctcatcg
    29641 ggaatgggat ggaccggggc ggagccgccc agctccacac atccagctac gacttcaacg
    29701 attcgatcgt gacggtcggc gccgcctatt gggttcagct ggtccgcgat gagttggggt
    29761 cgtgctgaaa tgtgtccgct ggggcgagtg acgcctccgt ggcgcgcgac gggcaaaact
    29821 atggggaaga caaaaacttt gggaaaggac atatgatgat ggatcgtcgc agttttcttt
    29881 tcgtcgccat gctggtggcg agctttgccg cttcgaccca gcagggccgg gccgacacgc
    29941 tcgccgatat cacggcacgc gggctgatcc gggtggccgt gccgcaggaa tcgccgccct
    30001 tcggcaccgt cggtccggac atgcagcttc agggcctcga tatcgatgtg gccaagctta
    30061 tcgcaacgaa gctcggggtg aagctggcgt tcgtcccggt tacgctgccc aatcgcatcc
    30121 cttatctgga gaccaacaag gccgatatcg tcatcgcgag tctgggcaag aacccggaac
    30181 gcgagaaggt catcgatttc accgatgcct acgcgccgtt cttcaacggc gtttttgcgc
    30241 cagcggaggt caatatagcg aggccggacg atctggccgg caaaacgctg gctgtcgcgc
    30301 gcggttcaat cgaggatctc gccctgacga agatcgcgcc gcccgccgca accatcaagc
    30361 gcttcgagga caacaacacc accatctcgg ctttcctgac cggccaggtc gacgccatcg
    30421 tcaccggcaa cgtgattgcc gcggcgatcc ttgagaagaa tccgcctcgc cggccggagc
    30481 tgaagttcat catgaacaag tccccgtgct acatcgggct cagaaagaac gacgacaatc
    30541 tgaagcgccg aattaacgaa atcatcgagg cggcgcggga ggacggcagc ctgaacagga
    30601 tcacgcggaa atggctgcat caggacgttc cggctgacct ctagacgctg cgacaccctc
    30661 tccggtgccg cgcgatcggc accggagctt cacatgggcc gagcgagagt atcatgcagc
    30721 atcacttcga ctacgactgg ctcctgagat actattcact gcttctgcac ggcatcgcca
    30781 tcactgtgga attgaccgtg gttgcggcag ccttcggtac tgcggttggc atcgtctgcg
    30841 cctgggtgcg gtgcttcggg aagaattggg cgcggctgct ggtgacgccg tacattgaat
    30901 tgatacgcaa cacgcctttc attatccagt tgttcttcat cttcttcggc ttgccttcga
    30961 tcggcatgcg cctcggcgaa ctgcaagcgg ccaatattgc cgtcgtcgtc aatctgggtg
    31021 cctatagctg cgagattatc cgcgccggaa tccaggcgac gccgcgagga caatgggagg
    31081 ccggctccag cctggcgatg agccatggtc aggtctttcg gcatgtcgtc cttgttcccg
    31141 cgctgcagcg catctggccg gcgctcacct cgcaggttgt catcgttatg ctcggctccg
    31201 cggtcgtctc gcagatcgca gctgtcgaca tcacctttgc cgccaacttc atacagtccc
    31261 ggtcattccg tgcattcgag gcctatttcg tggcgacggg catctatctt ggacttgcgg
    31321 tcgccgtgcg tgaaattctt gcggccatcg gacgctttgc ctttccgcgc cggatttcca
    31381 gatgatcgat ttcacgacat gggatgttct tcggaatctt ctcctggccg cccggtggac
    31441 ggttcttctg tccctggtct cgttgttcgg tggtggcggg cttggcctga ccctgctgtt
    31501 cctgcggatc ggcagggatc ggctcggccg cagaatggtg aaatggtaca tcgaattgtt
    31561 tcagggaacg ccgctgctaa tgcagctctt tctcgttttc ttcggcatcg gtcttttcgg
    31621 cgtcgatgtg ccggcatggc ttgcggccgc gatcgcgctg atcctttgga ccgccgcatt
    31681 cctggtcgag atatggcggg gctgcgtcga ggcgatcgca aagggacaat gggaggcgtc
    31741 gtcgagcctt ggcatggggc atctgcaaca gatgcgctac gtcattctgc cgcaggcgtt
    31801 gcgcatagcg attccgccga ccgtcggctt tgccgtgcag gtgataaagg gaacggctct
    31861 gacatccatt attggctttg tcgagctgtc gaaggccggc accgcaataa gcaatgcgac
    31921 gttcaagcct ttcctcgttt atggcctggt tgcgcttatc tacttcgcgc tttgctggcc
    31981 gttgtcgaag ttcagccgta aactggaagg gtggcttcat gtctctcatt gaaattgccg
    32041 atgtcagaaa gcgctatggg gcgctggagg ttctgagagg cgtcaatatc tcagtggcgg
    32101 ctggcgaggt cattgccgtt atcggcaaga gcggatcggg aaaatcgacc ctgctccgct
    32161 gcatcaacgg gcttgaggcg atcgacgggg gcaggataac cgttgctggc gagcaggtga
    32221 cggcggatga cgcccgtctc aaggcgctca ggctgaaggt tggtatgatt ttccagcagt
    32281 tcaacctgtt cccgcatgag acggcgggcg gcaatgtgat gatgccgcaa cgagttgtgc
    32341 gcaaggtgtc aaagggcgaa gcggaggcga ttgcgagtcg catgctggag cgcgtgggcc
    32401 tggcggacaa gttcgacgcc tatcccagcg aactatccgg tggccagcag cagcgcgtgg
    32461 ccatcgcccg cgcacttgcc atgcagccca tcgcgcttct atgcgatgag atcacctctg
    32521 cgcttgatcc ggagctggtt ggcgaagtgc tggccgtggt gcgcgagctg gccgcgcaag
    32581 ggatgacgtt gatcatggtc acgcatgaga tgaagtttgc atacgaggtg tgctcgcggg
    32641 tcgtcttcat gcatgagggg cgcgtccatg agatcgggcc gtcggagacg gtgttcacga
    32701 ggcccgcgac ggaggaactg cagaagttcc ttggcagggc ggcctgatgc acggtcttcg
    32761 gaagggcgcc tgccagggtg gcggcgcggc tgagagggat gcagtctccg ggcctggatt
    32821 gtcgccgacc cgcaattccc gggcgcccgc catccggtag cgctccggcg tcccggagga
    32881 acctgcgccg cagccgatct cgtcggggca tcacttggac gaactcgaag gactcgtcgc
    32941 cgggatcgtg gcggacatgg cggcgcaaac cgaacgcggc aatgtcgcaa gctacattcc
    33001 gcagcttggc agggccgatc cgctccaatt cggcatggcg gtgctgttgg gcgacggccg
    33061 gatgatctgc ggcggcgatg ccgatacgcg gttttccatc cagagcatct ccaaggtctt
    33121 taccttgacc ctggctttgg gcaaggccgg ggatgctctc tggcgccggg tcggccgcga
    33181 gccgtccggc catccgttca attccatcgt ccagctcgag tatgagcgcg gaaagccgcg
    33241 caaccccttt atcaatgccg gggcgctggc gctggccgat gtgattctgg ccggccatca
    33301 gccgcgcgag gccctgggcg agatactgcg tttgatccgg gatctggccg atgacgaaac
    33361 cattgccatc gaccacgatg tggcgcgatc ggagcaggcc acaggctttc gcaacgccgc
    33421 gctggcaaac tacatgcgcg cctgcgacat gctggagaat cctgtcgact acgtgcttgg
    33481 ggtctatttc caccattgcg ccatcgccat gtcctgccga cagctggcca aggccgggcg
    33541 ctacctggcc tttggcggcc ggcactcgga tcgcggcggg agggtgatat cgccggagcg
    33601 ggccaggcgg atcaatgcat tgatgttgac ctgcggccat tacgacggct cgggggagtt
    33661 tgccttccgg gtcggcatac cgggcaaaag cggggtgggg ggcggcattc tgggggtggt
    33721 gccggggcgg gcttcggtct gcgtctggtc gcctggcctc gatgcgcagg gcaattcccg
    33781 cctgggccgg ttggcactgg agcgcctgac ccggggcatg ggctggtccg tctttggagt
    33841 gtagcgggac cccatcgctg gaagcggcag tgggagggcg ccggcatgcg ccttacgggc
    33901 gccatggctt ggtgcgccgc gggaatgttt ggatttgaca gcaccgggat cggttgatta
    33961 tctaacaaac gttagaaacc tcggcatgac gggccgggcg ctgcgcgacc ggaccgggtc
    34021 cggccgcatg ccccgcatgg gaatggcctc ttggaatgag cgacgcgaag acggaatttc
    34081 ccgcggagcg ctatctgcag ccttcgactt acatcgacag cgaatcgccg gaggtgttgc
    34141 agctgacccg ggaggcgcta gcggggctgg acggcgcctc gacgaccgaa aggagcatcc
    34201 gtctgttcga ggcggtgcgg gacggcctgc gctatgatcc ctacagcttc gccgtacagg
    34261 ccgatgccta tcgggcgagt gcgatcgccc ggcaggagtc ggcattctgc gtaccgaagg
    34321 cgatcctgct gacggcctgc ctgcgggcgg ccggcatccc tgcggcggtc ggcttcgccg
    34381 atgtcaggaa ccatctgaac tcgccgaagc tggcggacct gatggggacc gatctgttca
    34441 tctaccacgg ctatgtccag ctctggctgg acggcgtggc ccgcaaggtg acgccagcct
    34501 tcaacatgga gctttgcgaa cggttcggcg tcgggccgtt gctcttcgac ggccgttcgg
    34561 atgcgctgtt ccacgagttc gacgcggaca accggcggca tatggaatat gtcaacgacc
    34621 gcggcatcca tctggacccg ccgatcgacg agcttctggc ggccttcagg acaacctatc
    34681 cgcgcctggt cgaattcacc gaaaggcgca tcgccggacc ggatgccgcc atcgaggatg
    34741 ccagcttccc ggggacggcg atccgcaaag gcgatgctcc ggcggatcgg gcggaagggc
    34801 gtttgagttc gtcgaggaac gggcttgcac aaaagggaga atacaatgag tgaccggtat
    34861 gcggcctatg acaagctgac gttcgactat ccggcaaagc gcgtcctgcg catgaccttc
    34921 gaccggccac agaccttcaa ctcggtcgat gccgagacgc atacgcagat gaccgatgtc
    34981 tggcgtgaca tcgaccgcga tccggacatc aacacggtga tcgtgaccgg tgccggcaag
    35041 gcgttttcgg ccggcggcga tttcggcctg atcgaaagca tggccggcaa cttcaatcag
    35101 gtcatggcga cctggaagga ggcgaaggac ctcgtctaca acatcatcaa ttgcaacaag
    35161 ccgatcatct cggcgatcaa cggtcctgcg gcgggtgccg gtctggtggt cggcattctc
    35221 gcggacatct ccatcgcggg caggaaggcc aagatcgtcg acggtcaccc gcgactgggc
    35281 gtggcggccg gcgacgtcgc cgcaatcatc tggccgcttc tgtgcggcat ggccaaggcg
    35341 aaatactacc tgctcacctg ccgcgcgatc tcgggagagg aggccgaccg gatcggcctc
    35401 gtctcgatgt gtgtcgagga cgaggtgttg caggacaccg cgctcgagat cgccagcgaa
    35461 ctcgccaacg gctcgcagag cgcgatccgc tggaccaaat acgcgctgaa caactggctt
    35521 cgccaggccg gtccgatctt cgacacctcg accgcgctcg agatgctcgg cttcatgggt
    35581 gacgacgtca aggaaggcgt cgcctcgcac cgggagaagc gcaagccgaa tttccgcacg
    35641 gattgcccga tctgacgcaa gccgggcgag gccgtagcac gaggcctgcc ccgccggcct
    35701 cgcctcaatc cgccatgttg cggtaccggt cgagcccgcc ggaatgcatc acgcgtccca
    35761 tcagcggcgc gccgatcatc cgttcggccg cctgcgccgc cgcaatcagc ttgtccaggt
    35821 cgatgcccgt cccgatgccg agttcgtggc agaggaacac ggcatcttcc gtgcagacat
    35881 tgcccgccgc gcgggcatgc ccgtggctgg cgaaggggca gccgccgagc ccggcgaccg
    35941 aggtctcgaa caggtcgaca cccatggcga gcgccgcatg gacattcgcg atcccgaggc
    36001 ctcgcgtgtc atggatatgc aatccgatgc gcgcctcggg cgcgatgtcg cgcagcccgc
    36061 cgatcatccg cctgaccgct tcgggattcg cccatcccat cgtatcggcg atcaccaggg
    36121 tggggatctc gatctcctct tcctgcgcca gacgcaggac gaaccggaaa tcgtccagga
    36181 ccttgtcctg cgggatggcg ccttcgaagt tgcatccgaa ggcggccatc acataggccg
    36241 tctccaccgt gcagccgttc tccttataga gccgtatcca gtcgcgctgg cgctcgcgca
    36301 tttcagccgc ggagcagttg ttgttcttcc gcgagaaggt gtcggaggcg tagaacagga
    36361 gccgcgggtc gatatgaacg tccttggccg ccagagcctt gcggaagccg ctgtcgttga
    36421 gccacaacgc ggtgtactgc accccttcga cgcgcttgat ccgcttgaag agttcgccgg
    36481 tatcggccat ctggggcaca tatcgcggac tgacgaagga tcccacctgg atgcgtttca
    36541 atccgctgtc gctgagcagg tcgatgaact cgacgcgttg ctcgatcgga tagatcttct
    36601 tttcgatctg gaacccttcg cgggggcctt cctcgcggaa ttcgacgaat ctcggatagc
    36661 cggtcatctc tcctgcacct ccaggggcgc gatctcggcg atcgtctgtc cacggtcgac
    36721 catgtcgcct tcgcggacgg cgatcctggc cgcgcggccc gatgccgtag ccttgatcga
    36781 gatctcaagc ttcatcgatt ccagaattgc caccgtatcg ccggcggcga ccgcatcgcc
    36841 ttcgtggacc gcgaccttga cgatcaggcc cgtcagtggc gagctcagca ccccatccgc
    36901 catcggctgg cgcgtcgctg cggcgagcgg aggcaagatg cgaaagccga ggcgtcggcc
    36961 ggcggtttcg atgtcgatct cgccggcgcg ttccgcaaca taggccgcac gcgcattgcc
    37021 gacgacatcc tcaacacgat gcgcgccgcc gattatgcgg tacgggaaac ggacccgttc
    37081 gaaaccaagc cggccttttc actgagcgct cgcgaagtgt ctccacacgt caatcgcatt
    37141 cgccttctct ggcaccgcat gcgcgagccg atccttgcgc gctttcccaa gccaccgggc
    37201 aggccgaacg atgcccgccc ttatctgaag caggtcgaag aaatctacgt caccgacgcc
    37261 tatcactcgc tttcgatcga aggctatcga gtcagccccg cattgatcga acgcgtgcgc
    37321 agcggaaact ggaatccgga cggtaatgac caggatcgcg aacaccgcga cgcactcgcg
    37381 gcaagaggct attggcaaac cgtgacagct cacagaagga ccggaggcgg acagaccgat
    37441 gagcgcggat accgaccatc ttgctgccga agctgcttgc tacgttctag agacgcgacg
    37501 gctcagcggc ggaaggtcag cgccgcaatg aagcgtctgt cgtgaatcac gacagacgtg
    37561 tcgcggagcc tgatcgagtt acaagagata tttgcctact tcaccacctt gtcgatgaat
    37621 ccgagcgtcg tcgcgttctc catgagggtt cgcacctttg cgcgatccag tccgccagag
    37681 acttctgcat cgatttccag ctttaacgag acatcactcc ccggcaacgt cgtaagctgt
    37741 tgtacgattg cctcaacaat ctggtgcatg tcgcgggctg gccggtcgga tgaaatcata
    37801 actgtgccaa tgaatcgggt aggcaatttt tctgcttgtt tggctccggc ttcttgtccg
    37861 ctttggtcac cagttgtcgt gccagtgcct gtcgcggttg gcccggtgcc cgctcctgtg
    37921 gactcggtcg tttctgccgt cggcctggga cgatgagctt cggctacgtc tggtttaacg
    37981 atcacgctat cgcggtcgat tacgacatgc gggttagccg ctttctcgat caacagcccg
    38041 agatatttct gcgtgccttc ttcccagcgc tcggcgtagg caaatgggcc gggcagcatg
    38101 cctgacacgg cggcctgaac agccttcacc aacactgcct gatttttgag gcggggcagg
    38161 tacgtgtagc ggttaaggta ttcccaaagg tccttcagca aaagatgcgg cttgtcattc
    38221 cagatgtagc gctggaggtc acgatcgagc ctggtcgggc cgagttccgg caacagacct
    38281 tcatcgctaa ccagtttttt gctggcacga gatagaagac cgtcctgagc gggcaccttg
    38341 ccagaggccc actcgacttc cgcctgagcg ctctcctggt gcggatagat caggtagcac
    38401 cagcattcgc gcagacgtgt cttcagagtc tcattcgcct cgacggattt cgccccggcc
    38461 aatgcgatat cacttgtctt cagatcgagg cgcttgtcgt ccttgacgac cgaagcccat
    38521 gcgagcgccg accggacggc atttttgaga ttatctaatt gtcgcgactc cgccgcgagg
    38581 aacaccagcg tgttccggta gacccgcggg gtcgatccgc gctgcatcag aatgtctttg
    38641 gcttcggtaa gcgcttccga accgtcgcgg cccgtgtggg ggtgtgctac gccgagcact
    38701 acggcccgga caccgccggg ttcatcgggt acgtccgcgg aggacgaagg ggcgacctgt
    38761 actgcgtcaa agtgaccgcg atcgctgatg gcattgatgt acttcgtcag ttcctgatcg
    38821 attgtcatca gcacgagcgc ttcttcgagc tgaccggcgc gatccgcggc aatgcgattc
    38881 aagctggccg acatcgaata ccagtagcgg ccaagatcgc tatgcatgaa cttggcctga
    38941 ttcgttagac ggcgcaacgc atcgccaaag atggccggtc gttcgcccgg ctggacgacg
    39001 ccaaggttga tttgtttgtc gtcgagaccc ttgttctctt ccttatgggt tggagcagag
    39061 cccatgaaaa tcgcacgagc gacgcgtcgc gtcgccgaat agcgattaag gttgggcgcg
    39121 gattggtcaa ttttgtaggg cgtcgagttc gccccatcga catcgccagc tatgatcgat
    39181 tgccagttca cgtcgagata gtgcagcagt tccggctcga cccgtgccga gctgatcgca
    39241 acgctgcccg gcatgatcat caccgaagga tcgttgctca tccatagctc atggatgacc
    39301 tgtgccataa ggcgcaagac gccacgcgtc cgctgaaatt tttccagcga tccccagctt
    39361 gtatagagct ggtcaaacag ctccgggtga atcggatatg ccttctccag cttccggcga
    39421 taatcctcgt cggcgcaccc ctgcgggaaa tcattcccgt tttcgcgata gagcttcgcg
    39481 aattgcttaa gtgtattgtc cttgtgatgg tgcctgtcgc ctgggatttc cttaaacagc
    39541 cggcgccgga caatctcata gctctcttcc tgagacgcgg gcctccacga cgactcgacc
    39601 cgtgagaatg tttgcttcag gcgcgccagt gcttcctgac caccttcgcc accaacctca
    39661 attagtgaag caggcagcga ggcgacgagg agcgtgccgg ggctggcttt agcctgcctg
    39721 cgcgtagccg ctccggcgaa gccaggttgc ccgccatcct ctacaccaac aacaccgggc
    39781 gcgggtggcg cgtccgtcaa atttcgccgc gcgcctcgcc ggatggcttg gcgcgcgggc
    39841 attctcacga atgctgatga agaacataaa cccgagcgta cttcccgcta ccgttctctg
    39901 cgggcttcag ttcccccgga cgccgtcccg ctgccgctca gcgcccggcg cagcgtaatc
    39961 gcatccacgc cgagacgggc tgcgacggct ttgcgcgcct gccccgatgc gaccgccgcc
    40021 cgcgcttctt caatttgcgc atcggtgagt ttgcgcgggc ggcccacgtg cttgccgtca
    40081 cgcttggcgg cggccatgcc ggcccttgtc cgctcggaaa tcagctcgcg ttcgaactcg
    40141 gcgagggcgg cgaagatgcc gaagatcagc cgcccgttcg ccgtcgtcgt gtcgatctcc
    40201 gcgccctgac cctccaaaac tttaaggcca accccttttg tcgtcagctc atgcgcgagg
    40261 ttcacgaggt gccgcaggtc gcggcccagc cggtcgagtt tccacaccac cagcacgtcg
    40321 ccgggctttg cggccttcag tgcgcgtttg agggcgggcc ggtttatcgc cgcgcccgat
    40381 atgcccttgt cctcgtagat gcgctcgcat tcggcgcgtt taagcgcctg ccgttgcagg
    40441 gcgagattct gatcctctgt ggaaacgcgc gcgtagccga ttttcatggc gaaccttcac
    40501 agctcaggcc cgcttgcgct gccgtcttca aagccgagcg gcttccgaaa acccgacgaa
    40561 aggggtggaa tgcacgcgtt gctgacacac tagcggagat ccgtgaaggc gatgccttcc
    40621 ataatccgga agtcagatct gagatcagaa agaagcgagc atatcgaaag cggaaacatc
    40681 cggaagggct tgggc
//
LOCUS       DASVDU010000245        28683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_215994, whole genome shotgun sequence.
ACCESSION   DASVDU010000245 DASVDU010000000
VERSION     DASVDU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28683
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215994"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF226_15955"
     CDS             complement(<1..285)
                     /locus_tag="VF226_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX7075534.1"
                     /translation="MQIPIIERRRIEAQLLKIIHDELVTRLGEAAAEEIIAESVRKSA
                     IEQGRQFASAEPEGTSMSTFVKLYDLWTMNDALEIDVLHQDAERFDFNVTR"
     gene            complement(610..1305)
                     /locus_tag="VF226_15960"
     CDS             complement(610..1305)
                     /locus_tag="VF226_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7075535.1"
                     /translation="MKIGIIGAGQIGGTLTRRLTALGHQVSVANSRGPETLADLAAET
                     GATAVSVRDAVRGVDLVVVTIPEKNVPNLPAGLFADTDERVVVVDTGNYYPRQRDGRI
                     EAIEAGMPESRWVEQQLGRPVIKAFNNIFAQHLMEAGRPAGTPGRIALPVAGDDDTAK
                     AVVTRLVDELGFDAVDAGGLDDSWRQQPGSPVYTTDFDADGVRRALAEASRDRTPEWR
                     ATPNSPGSFAAPA"
     gene            complement(1970..2095)
                     /locus_tag="VF226_15965"
                     /pseudo
     CDS             complement(1970..2095)
                     /locus_tag="VF226_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
     gene            2430..2990
                     /locus_tag="VF226_15970"
     CDS             2430..2990
                     /locus_tag="VF226_15970"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7075536.1"
                     /translation="MTKHPKKRDFPVSHVRRYLEPGPIVLVSSKWQDEANIMTMGWHT
                     VMEFTPSLVGCIIAGGNHSFHLIRNSGECVINLPTTALTDAVVGIGNSSGAEIDKFRE
                     FGLTAGEAREVEAPLIRECHANFECRLHDDTLIDRYNFFIFEVVRAHVAPSPKHPQTL
                     HYTGDGVFMVSGQIVNRRSQFRPGML"
     gene            complement(3154..4449)
                     /locus_tag="VF226_15975"
     CDS             complement(3154..4449)
                     /locus_tag="VF226_15975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7075537.1"
                     /translation="MSDKVFEPVDVAIIGAGIVGCSAAWHLARSGLKVSVFERGTVAS
                     EQSSRAWGFIRQQGRHEAELPLAAEANRLWVELTQRFGQEATQFTQGGILVPAETEAD
                     EERVISGHEIARKFNLGTTVLDKAGIQDLIPGLSGNWRSGLYTAGDAHGEPPTSTRTI
                     AEAARAAGARIHEHEPVIEIETQAGQVSGIVTINGRCRAPVVVIAAGIGAPMLARKLG
                     LNLPIQVITSSVGQTTAAEPFTRVALWGPTVAYRPRADGSYTLGNGYRGMGPDYEITP
                     NSFRNLRYFLPAYRRNWRLLRLTVGGEFINHLRASFGKEASARPLPEVKPNMRKVLRN
                     LAQFRGLFPHLGNVGLDRVWAGRIDLTPDAIPIIDRPSPNLSLFVAAGFSGHGFALGP
                     SVGKQLAEWIAANRPSLDLKAFRLSRFAEGDVSRAKQAL"
     gene            complement(4455..5477)
                     /locus_tag="VF226_15980"
     CDS             complement(4455..5477)
                     /locus_tag="VF226_15980"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX7075538.1"
                     /translation="MTSQLMIVDGMGSAALLPSAQVPPPMRNGKPWLDRALASGLTTM
                     NVTMGIKGVAQGTDDFRAYLHSVHGYLSYFELEPRLLHVQAASDIVRAKTEGRLGIIF
                     GCQGFDTKIENDPSLILIMARLGQRIVQLMYNEQSSIGCGALEANDTGLTEFGRICIR
                     EIDDCRMVVDLAHAGQRTAKEAIGFARGIPIISHANVRALVEHPRNVTDDVLKALAER
                     GGVVGITAYAPFCEKQPGVRPSIDDMIDHVAYVGERFGIDHVGIGSDFFEGESFIRFE
                     RFFRRRYPAIVGSYTMETVYTEGFEAIDDFALLPQALKRRGFTDAEIAKVLGGNFLRV
                     FEQVWG"
     gene            complement(5488..5838)
                     /locus_tag="VF226_15985"
     CDS             complement(5488..5838)
                     /locus_tag="VF226_15985"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF002571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HEX7075539.1"
                     /translation="MAGPELACRRRLHGQARRRFRYPPGRGRPQTPELRAGILEARLP
                     AGANIKKKDDDMPYVRIELIEGRSEDQKAAVAEAVTKALVELAGAKAESIFIVFDDVP
                     PHNWASGGKLLSRR"
     gene            complement(5721..7493)
                     /locus_tag="VF226_15990"
     CDS             complement(5721..7493)
                     /locus_tag="VF226_15990"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7075540.1"
                     /translation="MAVDVGAVDRTKGSAPVAMRPTLAGLNHMVSAGHYLAAHAGFGV
                     LEAGGNAVDAGVAAGIALSVVQSDFVNFAGVAPILVYMADTGEVSSISGLGWWPKAAS
                     LGFFVKEFGGNIPVGILRTVVPAAPDAWITALERFGTMSFAEVAASAIGFAESGFVMY
                     PLMAEMIATNLAGYNRWESSRQVYLPNGAAPRVGDVFRQRDLANTIRYMADEEKAHAH
                     RGRSAGLCAARDAFYRGDIARRIVDYHQAHGGFLSMADFDSFSVEVEPAIRSSFHGTD
                     LYACGFWCQGPSLLQILNMLEHRDLKALGHNTPDYIHLLVETTKLAFADREAYYGDPR
                     HLDVPAAALLSKDYAKRRVALVDDRSAVTGLAPAGDVPDGPRPPQIPPEIKGTVLGTG
                     TDPRIDTSFVCVVDRHGNAFAATPSDASNNTPVIPGTGLCPSSRGCQSWARPGHPAAV
                     APLRRPRLTPNPAIAIRPGRFVMPFGTPGGDVQSQAMLQVFLNIDLFGMDMQQAVEAP
                     RFATYSFPSSFEPHTVEINRLVLEDLIPAQTGDVLAEKGHEVQWWRDRNWRAGGVCTV
                     RLDVDSGIRRAAADPRRPSYALGY"
     gene            complement(7553..8380)
                     /locus_tag="VF226_15995"
     CDS             complement(7553..8380)
                     /locus_tag="VF226_15995"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075541.1"
                     /translation="MSLMKGFTGFRLSWLAAGFAILFLVAPFIVVVGASFDTGEAYFV
                     AFPPRGITFEWYSSIPSKYIEAAWTSFIVAVGVAIFSSVVGMCAALGIVRGNVWGKEL
                     FQSFFRLPIQIPLVVTGAVFLQFYYQVAAITGVNALGNLWGLVIAHLFVAIPYSVGSV
                     SAVLARVDPSIEEAAHSLGATHWSTFWRVTFPLMRPGVAAGMFYSFIVSFGDVPIAIF
                     LVSGDNMTLPVQIFLDMQFDFRPDMLAVSTLVVVVSAFIIIGAQRFAGLDLVLPGRR"
     gene            complement(8377..9198)
                     /locus_tag="VF226_16000"
     CDS             complement(8377..9198)
                     /locus_tag="VF226_16000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075542.1"
                     /translation="MWPAAALSFALLVLPQLGFVWLSFHQDLGLGQISDELTLANYWT
                     VLTDPLYLGSIWLTIYLSVATVVVVMLVGFPTAYALARMPARLSTGILGLLLTTSLIT
                     VVIKLMGLNIILGSSGMVNNLLLYLGIVSSPLQFINNELGVLIGLVQYTLPLFIMLLF
                     SVIQTIPLSLEEAAEVHGATRASLWRRIILPLAKPGLVAGALITFNMSMGAFTSAVLL
                     GGGRVRTIPVLIQQTIIQSTEYGLGAALSTTLLVLVFLINVAVGIWASRRVRRTP"
     gene            complement(9362..10462)
                     /locus_tag="VF226_16005"
     CDS             complement(9362..10462)
                     /locus_tag="VF226_16005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075543.1"
                     /translation="MASVSLRGVSKSFAGIDAVKHVTLDVAEGQFVVLLGPSGCGKTT
                     TLRMVAGFVQPTAGHILIDGKDVVGLPPRRRNIGMVFQNYALFPNMTVAENVGFGLRE
                     RKVPRAETRARVSELLELVRLSARADAYIGELSGGQQQRVALARALAFRPQVLLMDEP
                     LGALDLKLRESMQIELHRIQRNLGITTIMVTHDQQEAMSLADRIVIMADGEVQQTGTP
                     EDLYRAPRSTFVADFIGKNNLLSGRVTGATGDCCTVRIADGFEIQIIASGREAVGQPI
                     ALSVRPEQLCLNAAEPSSRFNTVYGTVESRRFLGNLIYYSVRLPHGETLLVESSANTA
                     PAEEGARVQVGWRPEAGIAFSLEADADAARPS"
     gene            complement(10554..11639)
                     /locus_tag="VF226_16010"
     CDS             complement(10554..11639)
                     /locus_tag="VF226_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7075544.1"
                     /translation="MLQRTRREFVGMSLAAPLAAIGLSVASRFSFAAELSGTTLRIAT
                     WGGSWRDSINKNVVSDLISSGVNVEYVLGNPEDNLAKLIAASRQGQVPFDAMEGFPHI
                     TNPMAKAGLIEKLNYDNLPNTKAAPKWARSEYEVVSVFTEDGVVYNVDKFKEAGIEPP
                     THYTDLTNPKLKGRVAFPDIGNAQHWNAVVGLAYESGGDEAHMDGAVKLVNEISPSYF
                     FSASTELATRFGSGDIWAAPWHAGWASRLKRSGVPVSVGYTSFGDRRAAIWQVPDYVV
                     TNSPNVAAAEAFINDLLAPENQFEHGRATGTIPVISQARIKLKDDPISKELLLLDDAD
                     LENIFQIDWNKLDEKAWRETWSREIHR"
     gene            complement(11810..13267)
                     /locus_tag="VF226_16015"
     CDS             complement(11810..13267)
                     /locus_tag="VF226_16015"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7075545.1"
                     /translation="MQLNDPGLFRQQCLVNGLWRDAASSARMVISNPADGAVLGTVPE
                     LSAEETRAAIEAAHGALPRWRKRTAKERAAILKRWSGLLLENIADLARIMTAEQGKPL
                     AEARSEITGAAGFIEWFAEEGKRAYGEIVPAAAPDRRTVVLKEPIGVVAAITPWNFPS
                     SMIARKCAPALAAGCTIVVKPAPQTPFSALALAALAERAGVPAGALNVITGDAGTIGP
                     QLTSHPKVRKLSFTGSTRVGKMLMAQCADTVKRVSLELGGHAPFIVFDDAEIETAVAQ
                     LMLAKFRNAGQACVGANRIYVQEPIYDRFLERLTAAVAALKVGNGLEEGVTQGPLISA
                     AAVTKVETHISDAVDKGARVLAGGCRHSLGGTFFEPTVLADVTDDMLTACEETFGPVA
                     SVLKFATEEEVFARANNTPYGLSSYVFTRDIGRVWRAAESLEAGIVGINVGVTASESA
                     PFGGVKESGIGREGSKHGLEEFLEIKYICIGGLAS"
     gene            complement(13545..14081)
                     /locus_tag="VF226_16020"
     CDS             complement(13545..14081)
                     /locus_tag="VF226_16020"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7075546.1"
                     /translation="MARVVKRRRSGEHLAEAFRQAMRQFAATVTVVTTGDRERRFGIT
                     ATAVTSAAMEPPSLLVCINRSASMHGPLTELGRFCVNILKTGQENVSRAFGGGCRQED
                     RFASDQWVIDDCGVPFLPAAQTVVFCKTAQSVECGTHTIFIGEVQRVLVQPEIAPLLY
                     CNGVFGALETSGDRSRIA"
     gene            complement(14074..15129)
                     /locus_tag="VF226_16025"
     CDS             complement(14074..15129)
                     /locus_tag="VF226_16025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7075547.1"
                     /translation="MDFGYYLPCYWPDTSYPMQNMYREMVAEAQFAEELGYCSFTIPE
                     HHFTNALVHPDALLSAVHIAAHTKRVPVITATTVLPFHNVPQLAGQIAQADCLTNGRI
                     QVGVGRGAYRYEFKRLQIPYDNARERFADSLDLLVDLLSKEDVSWDSDFYKIPATTIT
                     PRPVQKPHPKIWFAATTPGAIEYAAGRSFPVMTTPLRESFDKVVAQATAFQNGRAKSE
                     GSKEHLKLSMLIMLFVTDGRRQTEELVRHALGRHRRFVNVFGGEGEVERGAIKPYDVD
                     LTEADIERNLIIGPPEVCVDRLKAYAELGIDEIQLNMNFGASHAEVMRSLEMFATQVM
                     PHFAPKREEELLRQVHG"
     gene            15255..16649
                     /locus_tag="VF226_16030"
     CDS             15255..16649
                     /locus_tag="VF226_16030"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7075548.1"
                     /translation="MTKWQPDIENSGVEHYKAIVEAMGRDIASGALKPGERLPPHREL
                     AHALGLAVGTVSKAYMEARRRGFIHSRAGSGTFVREHPLRERRRVRTDREDTGRIDLS
                     FNGPVNAALHSEALQAALTKVAASETMAPFLQYHRPWLGHDRHRSAGSTWIEQFGLKT
                     DMADVAVVSGAQHAASVALIATATAGDLVVTDELCDPSIRLLAGALGLNLRGLKADEH
                     GLLPEAFENACQTEKVKAIICMPDHHSPTLVVMPEQRRRAIAEIARAHGVTLIENAVY
                     RPFLEAAPPPIASFAPEISYYCTSLSKIIAPGLRIGFLTAPSGKADELVLGMGATSWM
                     TPPLIAEVATQWIEDGTARRLVDWQRTELAERNRMAAEILDGFTCSALKSGLHIWLQL
                     PDRWRAATLAQEARARDVLVTPADAFAIGWVNAPHAVRISLGGATGREALVAGLRIIV
                     EILGRKVESSYLAI"
     gene            16700..17029
                     /locus_tag="VF226_16035"
     CDS             16700..17029
                     /locus_tag="VF226_16035"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7075549.1"
                     /translation="MIYELRHYTAKPGKEQNLANRFRGHTLPCFARIGFNLVDFWENC
                     ERAGDLWYLLQWESEGIKAELWQRLRNDEEWQRIKAASERNGPLIGRIESVLLTRPVW
                     VTAKGDG"
     gene            17203..18795
                     /locus_tag="VF226_16040"
     CDS             17203..18795
                     /locus_tag="VF226_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX7075550.1"
                     /translation="MRYHHSARYRSRRSPVLARNTVSTSHPLATQAGLAMLARGGNAV
                     DAALASAITLVVVEPTGNGLGSDAFCILWDGSELHGLNASGCSPAAWTPGRFAGEPQM
                     PRRGWESVTVPGAVSAWVDLSSRFGTLPFATLFEPAIEYAERGFHVTPVIAELWWRGA
                     EELKHQPGFAEAFMPNGRAPAAGELFRSPALGRTLRLIAETKGEAFYRGELADRIADF
                     AREHSAVLTRDDLASHENHWCGTVSQTFADVMLHEIPPNGQGIAACMALGILRELGVS
                     EFVSDSVDAYHLQIEAMKLAFADVHRYVADHRFMAEVTAGHLLDPTYLASRARLIDHK
                     HAQEFGPGAPRDGGTVCLATADERGMMVSYIQSNYSGFGSGIVVPGTGISLQNRGHGF
                     SLTPGHPNEVGPSKRPFHTIIPGFVTRGDEPLMAFGLMGGPMQAQGHVQMVLRTQAWG
                     QDPQTAADAPRWRVISGLDVAVEAAVGESLLADLAARGHAVISEAPDASFGFGGAQLV
                     HRIDGGYAAGSDPRKDGQAAGF"
     gene            complement(18966..19286)
                     /locus_tag="VF226_16045"
     CDS             complement(18966..19286)
                     /locus_tag="VF226_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075551.1"
                     /translation="MDPTADARRDAGLEIRKLAEEFERSDEAAFRRTRIVCWVVAAVI
                     TLVILVLLISGGDWRYLGAFWVALVGLIWAGYGLSRRRQRQQTDRLKALASRWLAGEP
                     PGPG"
     gene            19352..19540
                     /locus_tag="VF226_16050"
     CDS             19352..19540
                     /locus_tag="VF226_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075552.1"
                     /translation="MSRSRLSDSDLRDSGLDRADTPAVVAEVYTRDTGATPPKAAARL
                     TNDAGATSPRNGEEYIAA"
     gene            19642..20065
                     /locus_tag="VF226_16055"
                     /pseudo
     CDS             19642..20065
                     /locus_tag="VF226_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674967.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(20094..22031)
                     /locus_tag="VF226_16060"
     CDS             complement(20094..22031)
                     /locus_tag="VF226_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HEX7075553.1"
                     /translation="MKDHWLFTVKGKSRTLTGLPALIFSLTAAGFSAFYLYTSGFGLV
                     STETNRGLYLMLTSVLVFLLYPARSRSPNHRPSLFDWIWVAATVLSIGYWINQYVSYA
                     MFRVSAPSQWDLAMGAVAILAMLETSRRVLGPVMVAIALVFLAQLYFGAWLPGRLSHP
                     GMSVERILEFTFMSQGALFGVVAATFATFVFPFMIFGAFLERSGAGTFFMDLGTALAG
                     RWRGGPAKIAVMTSALFGSISGSSVANVVTTGTFTIPLMKRVGYQPHHAGAIEAIAST
                     GGQFMPPVMGAGVFILATLTQTSYLTIALMNVIPAVIFFMFVLAMVDLEAARTGLTGL
                     PREEVPDPRAVLRKGWHYFIPLIVIIGLLFYGYSPEFCAFWGTVAAFAVSWRGRETGM
                     RPTGVVQALIGGAQSNTSAGAAIGTLGLIIGGIVLAGLGLKFSAVLVEFAGGNLFFAV
                     ILVTIISIIIGMGSSTTGSYIILSVVAAPALINLGVAPVVAHLAVFYAACLSNITPPV
                     CVSAFAGAAIAGSDPMRTGFAALKFGTTLAIMPFTFAYIPEVLLQGPPLDVAWTTFSY
                     LLGNLALAMAIQGTEFLRGAIGLWRRTAFLVAAILMMFPTAAIIDAAGVALLIVSWAP
                     SFIGRAGAAAASSGAVPPRPR"
     gene            complement(22157..23116)
                     /locus_tag="VF226_16065"
     CDS             complement(22157..23116)
                     /locus_tag="VF226_16065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7075554.1"
                     /translation="MKRIAISTLTATALAVAAPALAQDRQDIAFGGSNPGGVMYYMVG
                     AAGTIISEQLPDINVTQVTTGGSTENAKRLIKGELDMGIVYGSHVYLAQKQEGPFKNM
                     PKGDMLRGVAKAYEGPTYFVTLEGSEISSLSDLEDKMVALGPPGSGTVFNCSNILRAL
                     GLLDKIEPSMMTFADAGRALANGQIDALCQSSAPAAAVTELAETKGVKILPYADEEMA
                     KITETYPFYHVGTMPETIYKGVAAVDLPYTTVYWVAHERVPAETVKEILELVYQPDVK
                     KRLAEGHKAWAQMAPDVANFLKLGVPMHPGAEAYYKETGQWPN"
     gene            complement(23169..24053)
                     /locus_tag="VF226_16070"
     CDS             complement(23169..24053)
                     /locus_tag="VF226_16070"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7075555.1"
                     /translation="MTVGKGIGWRSVLFVPGSRPDRFDKAITSDADAVCVDLEDAVAP
                     AHKDQARAAATAFLAHGGGGGSPDRVLRINSPRKAVGLRDLLAVIEARPSGGTLLIPK
                     VESAEELCWIDQLLTEAALDLTLVALLETPRAIDRAPAIVTAGPTRRLTAVMLGGLDL
                     SAELGASGTWESLLFARSRLVQAAAQAGIAAIDMPFVDVGDPEGCGSEARRALALGFT
                     AKMAIHPTQVPIINETYTPTPEEVARAQRVVRALADAPDGVLLLDGKMVERPVVLAME
                     RVLARARAAGLPPRRESS"
     gene            complement(24066..24971)
                     /locus_tag="VF226_16075"
     CDS             complement(24066..24971)
                     /locus_tag="VF226_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7075556.1"
                     /translation="MWSKEYWAKKGEVDLFMWRKRLDDPAEGVRRPVFFLVHGSSFSG
                     PSGFDMQIEGRDYSFMDKFAEYGFDVWALDHEGYGRSSRTGSNSNIAMGAEDLRAGME
                     VVRRETGQNRYAFYGQSSGALRAALFAEKHPEWVERLVLDAFVWTGEGAPTLIKRREK
                     LDEFRASNTRPVDRPFFHSIFNRDRPGTSETLVADKLADLELALGDRVPTGTYLDMCA
                     HLPVVDPEKIRCPVLIVRGEYDGIATEEDLVAFWMRLSNKDKQFVILPGQAHVAPLGI
                     NRHRFWHAVHAFLTMPPRRDPPAAS"
     gene            complement(25020..25838)
                     /locus_tag="VF226_16080"
     CDS             complement(25020..25838)
                     /locus_tag="VF226_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7075557.1"
                     /translation="MQAQDPALPGDMYAFIEAGSDALTRARAAVERGGEGAAIPLSEV
                     RLEAPFPRPIRNLICVGKNYYEHAHEFDSSGFNKSASGSAVPEEPIFFTKAPNSVIGP
                     DAAIPAHNDPTGSTDYEGELTVVIGAGGRNIPAEKASDHIFGYTIINDVTARTLQQRH
                     NQWFLGKSVDGFCPMGPAVVTRDEIPDITRSRLQTRVNGELRQDTTVSKLIFDIPTLI
                     ATLSRTMTLKPGELIATGTCAGVGIGFKPPKFLKPGDVVEILVDGVGVLSNPVA"
     gene            complement(25934..26917)
                     /locus_tag="VF226_16085"
     CDS             complement(25934..26917)
                     /locus_tag="VF226_16085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7075558.1"
                     /translation="MRERCLGSAGPVVSALGLGCMGMTPIYDPPDPEEAVRTIHAAID
                     AGVILIDTADAYGGGRNEELVGRTVAGRRDRVVLATKFGNIRRPDGTRSVNGRPEYVA
                     QACEASLKRLGVETIDLYYLHRVDPSVPIEDTVGAMARLVQQGKVRWIGLSEAGAQTL
                     RRAQAVHPLAALQTEYSLVTRDVEAEILPTCRELGIGFVAYAPIGRGLLAGRVRGEGD
                     LAENDRRRQLPRFQGENLRHNLTLVDRVADLAGRHGVSPAAIALAWLLARGDDVVPIP
                     GCSRRRTLMDSLAALDLRLAEAEIASLDAAFASGTVRGTRYPETQMRRVGL"
     gene            27027..28235
                     /locus_tag="VF226_16090"
     CDS             27027..28235
                     /locus_tag="VF226_16090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7075559.1"
                     /translation="MAFGGRDAKVLAVIGSGHAMSHFYLLALPPLFPLIRDDLDVSYA
                     ALGLLLTVLNVATGLFQVPAGILVDRLGARTILVAGLSLMAAAMAGIGLADSYWTMIP
                     LIVAAGIGNSVFHPADYAILARSVDQSRLGRAFSIHTFTGNIGFALAPPVMVALTAAL
                     GWRVALMAAGAISLLVLIGFLVWGGVLADDAPDGAAQARQDRSVDLRILLDSRILILF
                     LFFLSVAMVTSGVQSFSVAALQGFQGLDFTAANTVLTGFLVASAAGILIGGFVADRTK
                     RHGTVAVAAMLGAAALFLLSGLAALPFAVLFVAFVAIGLLQGVIRPARDMMVKAATPE
                     GATGRVFAFVSTGLNVGGAITPLLFGLVLDAGEPRLVFLLLSVFVLLGIGTIGVVGRK
                     RPAASSVPAE"
     gene            complement(28257..>28683)
                     /locus_tag="VF226_16095"
     CDS             complement(28257..>28683)
                     /locus_tag="VF226_16095"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX7075560.1"
                     /translation="AGHPKGRRIAVVADEEHEHRRGQPHAGLWLFDVTDLSAIAPVAT
                     FHVSELDSPWSRAPGRFGMHQFQEHIDGNLVFCAWFSGGLRVVDIADPARPAETGFFI
                     PEPVGGNPSPQTNDVEVDERGLVYLIDRNRGFDILEFEG"
BASE COUNT         5188 a         9319 c         9114 g         5062 t
ORIGIN      
        1 gcgcgtgacg ttgaagtcga accgctccgc gtcctgatgc agcacgtcga tttcgagggc
       61 gtcgttcatg gtccagaggt cgtagagctt tacgaacgtg ctcatcgagg tcccctcggg
      121 ttcggcagac gcgaattggc gaccttgctc gatggccgac ttccggaccg actccgcgat
      181 gatttcctcg gctgccgcct caccgagccg cgttacgagc tcatcatgaa tgatcttcag
      241 gagctgcgcc tcgatgcggc gccgttcgat gatcgggatt tgcattgctt cacctcatgc
      301 cattcttcgg tctcatccaa taatgcctga tacgaggaag gcgatcagaa tatcggcgag
      361 ttgcaagcac atgaagggaa tgcctgccgg cgctatggtg cgcagatgga aaatcgaagg
      421 tgataccggg ggtatccgat cgggcgcgct tgacgagaat ttccactaag ttcgtgtact
      481 cataaccgag ccgcagacaa cctttggccg ccgcgtgccg gcagcgggcc cgaatgccgc
      541 ttccggaccc ggtgcctcac catccggttc gtccgtccgc ggaaattccg cgggttaccg
      601 ccccgcgcgt caagcgggag cagcgaagct cccggggctg ttcggggtgg cgcgccactc
      661 gggcgtgcgg tcccggctcg cttcggccag cgcccgccgc acgccgtcgg cgtcgaagtc
      721 cgtggtgtag accgggctgc cgggctgctg ccgccaggag tcgtccagcc cccccgcatc
      781 aacggcgtcg aaaccgagct cgtcgacgag ccgcgtcacg acggccttgg ctgtgtcgtc
      841 gtcgccggca acgggaaggg cgatgcgccc gggcgtgccg gcaggccggc ctgcctccat
      901 cagatgctgg gcaaagatgt tgttgaacgc cttgatgacg ggccgaccca gctgttgctc
      961 gacccagcgg ctctccggca tgcccgcctc gatcgcttcg atgcggccgt cgcgctgtcg
     1021 cgggtagtag ttaccggtat cgacgacgac caccctctcg tccgtatcgg caaacaatcc
     1081 cgccggcagg ttcggtacgt ttttctcggg gatggtcacg acgaccagat ccacgccgcg
     1141 aaccgcgtcg cggaccgaga cggcggtcgc gccggtctcg gcagccaagt cggcgagcgt
     1201 ttcggggcct cgtgagttgg ccacggagac ctggtgcccc agcgcggtaa ggcgacgggt
     1261 gagcgtcccg ccgatttggc ccgcgccgat aatgccaatc ttcatgatgc ctcctcctat
     1321 ggaacagggt cgctgagatc cgagggacct cgtgcgcatc gggagagagt tcccgtttcg
     1381 acgagatatg ggtttgttat gtctcacggg caaacccgag caagccctct cctcgcccga
     1441 cgaccgctcg ttggaccgca ctcgatttcg agacgctcag cccttcgaag gcgatgtatg
     1501 agaccatcgg cggaacctcg gcatcgcccc ggtttgccgc ccgtccttca ccacgccggc
     1561 tctcacgcgc cacttcgctc gccgcgatcg gctcctccga gcggcgcgcg atcgtgattc
     1621 cgcgacggcc ggcaagcccc ctccggtcga aagaccttga agtgccgccg gaagtgcacg
     1681 agccggccaa cgatcctgcg ccggcgcccg tccggggccg cacaccgcag cgcggatccc
     1741 ggcagctcgg ccgttcggac gaaaccgggg agggagcggc cgcgccgaat acgccgaatc
     1801 acgcgcccga cccggtcctg gcgagcgcct tggggccggg cgtcggaccg atcgaaactt
     1861 acgcggcgcg gcgctcgtcg cttcgcgctt gggcctgtgc cggggtctgt accggctcgg
     1921 cggcggccgc gactgccggc gcgcgccggt cttccctgcg ggtgaccacg cctgccacca
     1981 cggccgggat gtcggcgcgg ttgatgccga tgtcgcgcag ttcgcggtcg gtcagccggg
     2041 tcagctcctc gtaaagggca tgctggcgct tggcgtcggc gaaggcctgt gccacgccgc
     2101 ccaggccgcg aaacacggcc cgcacggccc tgatcagggc gccgccgatg atctcggcgc
     2161 ggtcgcggcg cgcctgcttg cggatccgct ccgcctcatc aaaatcgatt cgatcaaatt
     2221 cgtgcatacc gggcctccgt cacgccgtcc gctccccctt ggagcggcac atccaccttc
     2281 aactcgcccg ataagtggcc attcttttgc gccgcagtag gcttgctttt gcatcgcaac
     2341 aatgcaggaa ttgcagggcc ttgaaagaca ttccggtcga gcgggcttca cgcgcttccg
     2401 ctggacccga tcgccggaga ggatcattca tgacgaagca tcccaagaag agagacttcc
     2461 cggtaagtca cgttcggcgg tacctggagc ccggcccgat cgtgctggtg tcttcgaaat
     2521 ggcaggacga ggcgaatatc atgacgatgg gctggcacac ggtcatggaa ttcacgcctt
     2581 cgctcgtcgg atgcatcatt gccggcggca atcacagctt ccacctgatc cgcaatagcg
     2641 gcgaatgcgt catcaaccta ccgacgacgg cgctgaccga tgccgtcgtc ggaatcggca
     2701 acagctcggg agcggagatc gacaagttcc gggaattcgg cctgacggcc ggcgaggccc
     2761 gagaggtcga ggcaccctta atccgcgagt gccacgccaa tttcgaatgc cggctccacg
     2821 acgacacgct gatcgataga tacaacttct tcattttcga ggtcgtgcga gcgcacgtcg
     2881 ccccatcgcc gaagcacccc caaacgctac actacactgg cgacggtgtg ttcatggttt
     2941 ctggccagat cgtcaaccgg cggtcgcagt ttcggccggg gatgttgtga aaggcctgta
     3001 gcatatcgac gaccggcatg gctgagccga accggccgct ctgcgagggg agccggacaa
     3061 gcgaccctgc gtccggctcc aaggcgccat gagtagcctt tcggccaaag aaatccgggc
     3121 tgctcggatg agcgatcctc ccgcaaaata ggcttacaag gcctgcttcg cgcggctcac
     3181 atcgccttct gcaaaccgag aaaggcgaaa cgccttgagg tccagagacg gtctattcgc
     3241 agcaatccat tccgcaagtt gcttcccgac agatggtccc agagcaaatc catgcccact
     3301 gaacccggca gcaacgaaga gagataaatt cgggctgggc ctgtcgatga tcgggatggc
     3361 atccggcgtc agatcaatgc gtccagccca gacacgatca agccctacat tacccagatg
     3421 cggaaacagt ccacgaaact gcgctagatt cctcagtacc ttgcgcatgt tgggcttgac
     3481 ctccggtaat ggccttgcag atgcttcctt gccgaatgat gcgcggagat gattgatgaa
     3541 ctcaccgcca accgtgagcc gcaggagccg ccaattgcgc cggtacgcgg gtaggaagta
     3601 gcgcagattg cgaaaggagt tgggcgtgat ctcgtagtcg gggcccattc cccgatagcc
     3661 attgcccaga gtgtagctgc cgtcggcccg cggacggtag gcgaccgtcg ggccccatag
     3721 agccaccctg gtgaatggtt cggctgcggt ggtctggccg accgacgagg tgataacttg
     3781 aatcggcagg ttgagcccga gcttgcgggc gagcatcggg gcaccgatgc cagcggcgat
     3841 taccacgaca ggggctctgc agcgcccgtt gatcgtcacg atgccgctca cctgaccggc
     3901 ctgggtttcg atctcgataa cgggctcatg ctcgtggatt ctggcgcctg cggcgcgggc
     3961 tgcttcagcg atcgtccgcg tggaggtcgg cggctctcca tgagcatcgc ccgcggtgta
     4021 gagaccggag cgccagttgc cactcaggcc ggggatgagg tcctggatcc cagccttgtc
     4081 gagaaccgtc gtgccgagat tgaacttgcg ggcgatctca tgcccgctga tcacccgctc
     4141 ctcatccgct tcggtctcgg ccggcacgag aattccaccc tgggtgaact gcgtcgcctc
     4201 ttggccaaag cgttgcgtga gttcgaccca caagcgattg gcttcggcgg cgagcggcag
     4261 ctcggcctcg tggcggcctt gttggcggat gaacccccag gcgcggctcg actgctccga
     4321 tgcgacggtt ccgcgctcga agactgaaac cttgagccca ctccgggcga gatgccaggc
     4381 ggcggagcac ccgacgatgc cggccccgat gatcgcgacg tcgaccggct cgaagacctt
     4441 atcggacatg gcggttatcc ccacacctgc tcgaaaacgc ggaggaagtt tccgccgagc
     4501 actttcgcga tctccgcatc cgtgaatccg cgccgcttca acgcctgggg caagagcgcg
     4561 aagtcatcga tagcctcgaa cccctcggta tagacggtct ccatcgtgta ggagccgacg
     4621 atcgccggat aacggcgccg aaagaagcgc tcgaaccgga tgaacgactc gccctcgaag
     4681 aagtcggagc cgatgccgac atggtcgatc ccgaagcgct cacccacata ggcaacgtga
     4741 tcgatcatgt catcgatgct tggcctcacc cccggctgct tctcgcagaa gggtgcataa
     4801 gccgtgatcc cgacgacgcc gccacgctcg gcgagcgcct taagtacgtc gtcggtcacg
     4861 tttcgcggat gctcgaccaa ggcgcgcaca ttggcgtggc tgatgatcgg gatgccccgc
     4921 gcgaagccga ttgcctcctt tgcggtcctc tgcccggcat gggccagatc aacgaccatg
     4981 cgacaatcgt cgatctcgcg gatgcagatc cggccgaact cggtgaggcc cgtgtcgttg
     5041 gcctcgagcg cgccgcatcc aatcgagctc tgttcgttgt acatgagctg cacgatgcgc
     5101 tgtccgagcc gcgccatgat gagtatgagg ctcggatcgt tctcgatctt ggtgtcgaac
     5161 ccctggcagc cgaagatgat cccaagccgc ccctccgtct tggcgcgcac gatgtcggac
     5221 gcggcctgga cgtggaggag ccggggttcg agctcgaaat aggaaagata gccgtggacg
     5281 gagtgaagat aggcgcggaa atcatcggtg ccctgggcga cacccttgat acccatcgtg
     5341 acgttcatcg tggtaagacc ggaggcaagt gcccggtcga gccacggctt gccgttgcgc
     5401 atgggtggcg gcacctgcgc gctcggcaga agcgccgccg aacccatccc gtcgacgatc
     5461 ataagctggg aagtcatttt cgccgcctca gcgccgcgac agcagcttgc cgccgcttgc
     5521 ccagttgtgc ggcggaacat cgtcgaacac gatgaagatg ctttcggcct tcgcccccgc
     5581 aagctccacc agggcctttg tgaccgcttc cgcaacggcg gccttctggt cttcgctccg
     5641 cccttcgatg agctctattc ttacataagg catgtcgtcg tccttcttct tgatgttggc
     5701 gccggccggc aggcgcgcct ctaatatccc agcgcgtagc tcgggcgtct ggggtcggcc
     5761 gcggcccggc ggataccgga atcgacgtcg agcctgaccg tgcagacgcc gccggcacgc
     5821 cagttccggt cccgccacca ctggacctca tgcccctttt ctgcgagcac atcgcccgtc
     5881 tgcgcgggga tgaggtcttc gagtaccaat cggttgatct ccaccgtatg gggctcgaag
     5941 gaggagggaa aactgtaggt cgcaaagcgc ggggcctcga ccgcctgctg catgtccatc
     6001 ccgaacaggt cgatgttgag gaacacctgg agcatggcct ggctctggac gtcacccccg
     6061 ggcgtgccga acggcatgac gaaccggccg ggccggatag cgattgccgg attgggtgta
     6121 aggcgcggac gcctgagtgg tgcgaccgcg gcggggtgac ccggccgcgc ccaggattgg
     6181 caccctctgg aagatgggca caggcccgtt ccaggaatga cgggcgtgtt attggaagca
     6241 tcgctcggcg ttgcggcgaa agcgttgccg tgtcggtcaa ccacacagac gaacgatgta
     6301 tcgatcctgg gatcggtgcc ggtaccgagg accgtcccct tgatctctgg cggtatctgc
     6361 ggtggccgcg gcccgtccgg aacgtctccc gcgggcgcaa gcccggtcac ggcggatcga
     6421 tcatcgacga gcgccacgcg ccgttttgca tagtccttcg acaggagcgc cgccgcggga
     6481 acgtcgagat ggcgcgggtc accgtaatag gcctcgcggt cggcaaaggc gagcttcgtc
     6541 gtctcgacca gaaggtggat gtagtcgggc gtattgtggc cgagggcctt gaggtctcga
     6601 tgctcgagca tgttgaggat ctgcaggagc gagggcccct ggcaccagaa gccgcaagcg
     6661 tagaggtcgg tcccatggaa gctcgaacgg atcgcgggct cgacctcgac ggagaacgag
     6721 tcgaagtccg ccatcgaaag gaagccccca tgcgcctgat gatagtcgac aatccgcctt
     6781 gcgatgtctc cgcgatagaa agcgtcccgc gcggcgcaca ggccagcgct tcggccgcga
     6841 tgtgcgtggg ctttctcttc atccgccatg taccggatgg tgttcgcgag atcccgctgg
     6901 cggaagacgt caccgacgcg cggagcggcg ccattcggga ggtagacctg cctactcgac
     6961 tcccagcggt tatagcccgc gaggttcgtc gcgatcattt ccgccatcag cggatacata
     7021 acgaagccgc tctccgcgaa cccgatggcg gaggccgcga cctccgcgaa gctcatggtg
     7081 ccgaaacgct ccagcgcggt aatccaggca tccggcgcgg cgggcacgac ggtccgcagg
     7141 atgccgacgg gaatatttcc tccgaattcc ttcacgaaaa agccgagcga tgccgccttc
     7201 ggccaccagc cgagcccgct gatgctggag acttccccgg tgtcggccat ataaacgagg
     7261 atcggagcaa cgcccgcgaa attgacgaaa tcgctttgca cgaccgaaag agcgatgccc
     7321 gcagcgacgc cggcatcgac cgcgttgccc cccgcttcga gaacgccgaa gccggcatga
     7381 gccgccagat agtgcccggc cgagaccatg tggttgaggc ctgcgagagt gggccgcatg
     7441 gcgaccggcg cgctgccctt ggtccgatcg accgccccca catcaactgc catgtccgcg
     7501 cccttctttc tatttgtatt cgcaaagcag tcggcgccgc cggactcgct cgtcatctcc
     7561 tgcccggtag caccagatcc agcccggcaa aacgctgagc cccaatgatg atgaatgccg
     7621 agaccaccac gaccaaggtc gagacggcaa gcatgtccgg gcgaaagtcg aactgcatgt
     7681 cgaggaagat ctggacgggc agcgtcatat tgtccccgct caccagaaag atggcgatcg
     7741 ggacgtcgcc gaaggaaacg atgaaagaat agaacatccc cgcggcgaca cccggccgca
     7801 tcaacgggaa ggtcacgcgc cagaaggtcg accagtgggt cgccccgagg ctgtgggcgg
     7861 cctcttcgat gctgggatcg acgcgggcca gaaccgcgga caccgagccg acactgtagg
     7921 ggatggcgac gaacagatgg gcaatgacga ggccccagag attaccgagc gcgttgacac
     7981 cggtgatcgc cgcgacttgg tagtaaaact gcagaaacac ggcgcccgtc acaaccagcg
     8041 gtatctggat cggcagccgg aagaacgact ggaacagctc cttgccccac acattccccc
     8101 ggacaattcc gagagctgca cacatcccga cgacggagga aaagatcgcc acgccgacgg
     8161 cgacgataaa gcttgtccac gcggcctcga tgtacttgga cggtatgctc gaataccact
     8221 cgaaggtgat cccgcgcggc gggaaggcaa cgaaataggc ctcgccggta tcgaacgagg
     8281 cgcccaccac cacgatgaag ggtgcaacca ggaacaggat ggcgaagccg gcggccagcc
     8341 aagacaacct gaagccggtg aatcctttca tgaggctcat ggagtcctcc gcactcgtcg
     8401 gcttgcccag atgccaacgg cgacattgat gaggaaaacg agcacgagca aggtcgtcga
     8461 gagcgccgcg ccgagcccgt attcggtgct ctggatgatc gtctgctgaa tgagtacggg
     8521 gattgtccgg accctgccgc cgccgagcag gaccgcggag gtaaaggcgc ccatgctcat
     8581 gttgaaggtg atcagcgcgc ctgcaaccaa tcccggcttg gcgagaggca ggatgatacg
     8641 gcgccagagc gatgcgcgtg ttgctccatg gacctccgcc gcctcttcaa ggctgagcgg
     8701 aatggtctgg atgacgctga acaggagcat gatgaacagt ggaagcgtat actgtaccag
     8761 cccgatgagg accccgagct cgttattgat gaactggagc gggctcgaga caatcccgag
     8821 atacagcagc aggttgttga ccatcccgga tgagcccagg atgatgttga gccccatgag
     8881 cttgatgacc acggtgatca gcgatgtcgt aagcaggagc cccagaattc cggtcgagag
     8941 ccgtgccggc attcgagcga gtgcataggc tgtcgggaag ccgacgagca taaccaccac
     9001 aacggtcgcg accgataggt agatcgtgag ccagatggat ccgagataaa ggggatcggt
     9061 cagaacggtc cagtaatttg cgagcgttag ctcgtccgat atctggccga gaccgaggtc
     9121 ctgatggaag ctcagccaga cgaagccgag ctgaggaaga acgagaagcg caaacgagag
     9181 cgcggcggct ggccacaaga agcgctccca acgcactcgg tgcggatcga cggcgtgcgc
     9241 attccgaacc ttacccggcc agcccgcacg ctgtttctgc tccgctctgt tggccatgct
     9301 catcgggcgc gccgcatctc ggtatcagct tcctcgaatc taaactttcg ggggcggcag
     9361 cttaggaggg acgcgccgca tcggcgtctg cttccaagga gaatgcgatt ccagcttccg
     9421 gcctccagcc gacctggacc cgggctccct cctcagctgg cgcagtgttt gccgagcttt
     9481 cgacaagcaa cgtctcgcca tgcggcagac gaaccgagta gtagatgaga ttgccgagaa
     9541 agcgccggct ttcgacggtg ccgtaaaccg tgttgaaccg cgaagacggc tctgccgcgt
     9601 tcaggcaaag ctgttcgggg cgaaccgaga gagcgatcgg ctgccccacc gcctcccttc
     9661 ccgatgcaat gatctggatc tcgaacccgt cggcgatccg gacggtgcag caatcgccgg
     9721 tcgcgccggt tacccggcca ctgagcagat tgttcttgcc gatgaaatcg gccacgaacg
     9781 tgctacgcgg tgccctgtag agatcctccg gcgttcccgt ctgctggacc tccccgtcgg
     9841 ccatgatgac gatgcggtcg gccaggctca tggcttcctg ctgatcatgg gtcaccataa
     9901 tcgtagtgat gccgaggttg cgctggatcc tgtgcagctc gatctgcatc gactcccgca
     9961 gcttcaggtc gagcgcgccc aggggctcgt ccatgagcag gacctgcggg cggaacgcaa
    10021 gggctcgcgc gagcgccacg cgctgctgct ggcctcccga cagctcgccg atataggcat
    10081 ctgcgcgcgc cgaaagccgg accagctcga gaagctcgct cacacgcgcc ctggtctcgg
    10141 ctcgcgggac cttcctctcc ctcaggccga acccgacatt ctccgcgacc gtcatgttgg
    10201 gaaaaagggc atagttctga aagaccatgc caatgttgcg gcgcctcgga ggaaggccga
    10261 caacgtcctt gccatcgatc aggatatggc cggcggtggg ttgcacgaag cctgcgacca
    10321 tgcggagcgt cgtcgtcttt ccgcatcccg aggggccgag gagcacgacg aactgcccct
    10381 cggcaacgtc cagggtgacg tgtttgacag cgtcaatacc ggcaaaagac ttcgagacgc
    10441 cacgcagaga tacgcttgcc atgcccgccc ttttcacacc tacggatcag tttcaaaccc
    10501 gcctaggcga cggattggac taagactctc cgcgcatggg agtaaatgca gggtcagcgg
    10561 tggatctcac ggctccaggt ttcacgccat gctttctcgt cgagcttgtt ccagtcgatc
    10621 tgaaaaatat tctccaggtc ggcatcgtcc aggagcagga gttccttgct gatcggatcg
    10681 tccttgagct tgatgcgcgc ttggctgatc acggggatcg tgccggtcgc ccttccatgc
    10741 tcgaactgat tttcgggcgc cagcaagtcg ttgatgaatg cctccgccgc tgcgacgttc
    10801 ggcgaattgg tgacgacata gtccggaacc tgccagatcg ccgcgcggcg atcaccgaat
    10861 gatgtgtagc cgacgctcac cggaacgccc gaacgcttca gccgggaggc ccatccggca
    10921 tgccatggag cagcccagat gtcacccgag ccgaaccggg tggcgagttc ggtcgatgcg
    10981 gagaagaaat agcttggcga gatttcgttc acgagcttga cggcaccgtc catatgcgcc
    11041 tcgtcgccgc cgctctcgta ggcaaggccg acgacagcgt tccaatgctg cgcattaccg
    11101 atatcgggaa acgcgacccg ccccttaagc ttcggattcg tcagatcagt gtaatgagta
    11161 ggcggctcga tgcccgcctc cttgaacttg tcgacattgt agacgacgcc gtcctcggta
    11221 aacacactga cgacctcgta ttcgctgcgc gcccatttcg gcgcagcctt ggtgttgggg
    11281 aggttgtcgt agttcagctt ctcgatgagc ccggccttgg ccatgggatt cgtgatgtgc
    11341 gggaaaccct ccatcgcatc gaacggaacc tggccctgcc ggctcgcggc gatgagctta
    11401 gcgagattgt cctcgggatt gccaaggacg tattcgacat tcacccccga cgagatcaga
    11461 tcgctcacga cattcttgtt gatggaatca cgccagcttc caccccaggt cgcaatacgc
    11521 aatgtcgttc ccgacagctc ggccgcgaaa ctgaatcgcg aggccacgct gaggccgata
    11581 gcggcaagcg gcgccgcgag gctcatgccg acgaattctc ggcgcgtacg ctgaagcatc
    11641 gagtttcctc ccattttgtg cattcccaaa ttatgtttca ctccaccgta gcacagcttg
    11701 cgaccgagtc caacaaatgt cccaatccat ttgccatcat agggactatt gcacccgagc
    11761 gtactgctac aatgcgccct cgcccgtctg ccgtcgccgt cggttcggct tagctggcga
    11821 gcccgcctat acagatatat ttgatctcga ggaactcctc gaggccgtgc ttcgatccct
    11881 cgcgcccgat tcccgattct ttcacgccgc cgaacggggc gctctcgctc gcggtcacgc
    11941 ccacattgat cccgacgatc ccggcttcga ggctctccgc cgcgcgccag acgcggccga
    12001 tatctcgcgt gaagacgtag gaagagagac catatggggt gttgttggct cgggcgaaaa
    12061 cctcttcttc cgtggcgaat ttcagcacgg aggcgacggg accgaatgtc tcctcacaag
    12121 ccgtcagcat atcgtcggtg acgtcggcaa gcacggtcgg ttcgaagaat gtgccgccga
    12181 ggctgtgacg gcatccgccc gcgaggactc gggctccctt atccaccgcg tcggagatat
    12241 gggtttcgac cttcgtgacg gccgccgcgc tgatgagggg accttgcgtg actccctcct
    12301 cgagcccgtt gccgaccttg agtgcggcaa ccgccgcggt caacctctcg agaaagcgat
    12361 cgtagatggg ttcctgcacg tagatgcggt tcgcaccgac gcaggcctga cctgcattgc
    12421 ggaacttggc aagcatgagc tgagcgaccg ccgtctcgat ttcggcatca tcgaagacaa
    12481 tgaacggggc atgccccccg agttcgaggg acacccgctt caccgtgtcc gcgcattgcg
    12541 ccatcagcat ctttccgacg cgcgtcgacc cggtgaagct cagcttgcgc accttcgggt
    12601 gggaagtgag ctgcggtccg atggtgccgg cgtcgccggt gatgacgttg agtgcccctg
    12661 ccggaacacc tgcccgctcg gcgagagccg ccagtgcgag cgccgaaaac ggggtctgcg
    12721 gggcgggttt caccacaatc gtgcatcccg cggcgagggc gggcgcgcat ttgcgcgcga
    12781 tcatggagct cggaaagttc cagggcgtta ttgcggcaac aacgccgatc ggctccttca
    12841 gcacaacggt cctccggtcc ggcgcggccg ccggcacgat ttcgccatag gcccgctttc
    12901 cttcctccgc gaaccactcg ataaagccgg cggcgccggt gatttcgctt cgcgcctccg
    12961 cgagcggctt gccctgctcg gctgtcatga tgcgggcaag atcggcgatg ttctcgagca
    13021 gaagcccgga ccagcgcttg agtatggcgg cgcgctcctt ggctgtccgt ttgcgccatc
    13081 gaggcagcgc cccatgcgcc gcctcgatgg cggcacgggt ctcctcggcg gaaagctcag
    13141 gcacggtgcc aaggacggcc ccgtccgcag gattagagat caccatccgc gcggaggagg
    13201 ccgcatctct ccacaaccca tttacgagac actgttggcg aaagaggccc ggatcattca
    13261 gctgcatact ggtctccgat cggcttctat gctcgtgttc ccagaaagcc gcaaatcggg
    13321 cccgccgcgt atggaaccaa gagcgccgct cggatccaag cggttgcgcc gaaacaagcc
    13381 cctcgtggaa gctccatggc tcgagaggtg atgctccggc ctgctgcttg ccaaaagacg
    13441 caccgcatcc gaaaacgcca cgtacagtcg gcacatcgca cgaaggccgg cgtcacgcga
    13501 atatctcatg gtggggatca tagttaacct cttggaacga tccgtcacgc gattcgcgat
    13561 cggtcgccac tcgtctcgag tgcgccgaaa actccattac aataaagaag cggcgcaatt
    13621 tccggctgga cgagaacccg ttgcacttct ccgataaaaa tggtgtgggt gccgcattcg
    13681 acagactgcg ccgtcttgca gaagaccacc gtctgtgcgg cgggcagaaa gggaacgccg
    13741 caatcgtcga tcacccattg gtcggatgcg aaacgatcct cctgccgaca cccgccgccg
    13801 aaggcgcgcg agacgttttc ctgcccagtc ttgaggatgt tgacgcagaa gcgcccaagc
    13861 tcggtgagtg ggccatgcat cgacgcgctt cgattgatgc agacgaggag cgacggcggc
    13921 tccatggcgg ccgatgttac ggccgtcgcg gtgatgccga aacgccgttc ccgatccccg
    13981 gtcgtgacga ccgtgacggt cgccgcgaac tgtcgcatcg cctgacgaaa tgcttcggcg
    14041 aggtgctcac ccgaccgccg gcgcttgacg actctagcca tgcacctgcc gaagaagctc
    14101 ttcctcgcgc ttcggcgcaa agtgcggcat gacctgggtc gcgaacattt ccagggaccg
    14161 catcacctcc gcgtgggagg cgccgaaatt catgttgagc tggatctcgt cgatgccgag
    14221 ctcggcatac gccttgagtc gatcgacgca aacctcgggt gggccaatga tgaggttcct
    14281 ctcgatgtca gcctcggtca gatcaacgtc atagggcttg atcgcgccgc gctcgacctc
    14341 gccttcaccg ccgaagacgt tgacgaagcg ccggtggcgg cccagcgcgt gccgtaccaa
    14401 ctcttctgtc tgtcgccggc cgtccgtaac gaacagcatg atcagcatgg acagcttaag
    14461 atgctccttg gagccttcgc tcttggcacg cccgttctgg aacgccgtag cctgcgccac
    14521 gaccttgtcg aacgattcgc gcaagggcgt cgtcatcacc gggaacgagc ggccggcggc
    14581 atattcgatc gctccggggg tcgtcgcggc aaaccagatt tttggatgtg gcttctggac
    14641 cggccggggc gtgatcgttg tcgccggaat cttgtaaaaa tcgctatccc agctcacgtc
    14701 ctccttcgac agaagatcaa ccagcaggtc gagcgagtcg gcgaatctct cgcgagcatt
    14761 gtcgtatggg atctgcaggc gcttgaactc ataccgatac gccccccggc cgacgccgac
    14821 ctggatacgt ccgttcgtca ggcagtctgc ctgggcgatc tgacccgcta gctgcggaac
    14881 gttgtgaaac ggcagaacgg ttgtcgcagt aatgacagga acacgcttcg tgtgagcggc
    14941 aatatgcacg gcgctgagca atgcatctgg atggaccagt gcgttcgtga aatggtgctc
    15001 cggtatcgta aaggaacagt agccgagctc ttcggcgaat tgtgcttcgg caaccatttc
    15061 ccggtacata ttttgcatcg ggtatgatgt atcgggccaa tagcagggca gatagtagcc
    15121 gaaatccatt cctcgatcct ccgtcatcag accgaagcag gtctgtgctg gcgtaaagca
    15181 ttcaagtggt gcattctctc cttccgaatg agataatctg gcctgaatgt accagtcaac
    15241 acgaaaagcg tattatgaca aaatggcaac ctgacatcga gaactccggt gtcgagcatt
    15301 acaaggccat cgtcgaggcc atgggacggg atatcgcttc aggagcgctg aagccgggcg
    15361 agcgccttcc tccccatcgg gagcttgcgc acgcgctcgg ccttgcggtg gggacggtga
    15421 gcaaggcgta catggaagcc aggaggcgtg gcttcattca tagccgcgcc ggcagcggca
    15481 cctttgttcg cgagcacccg ctgcgcgagc ggcggcgcgt aagaacagac agagaggaca
    15541 ccggaaggat tgatttgtcg ttcaatggcc cggtcaatgc ggcgctccat tcggaggcgc
    15601 tgcaggcggc gcttacgaag gtggctgcct ccgagacgat ggccccgttc ctgcaatatc
    15661 accgtccctg gcttggccat gaccggcatc gcagcgccgg gtctacatgg atcgagcaat
    15721 tcggattgaa gaccgacatg gccgatgtcg ccgtggtttc cggggcacag cacgcggcct
    15781 cggtcgccct catcgcaacg gcgactgccg gcgatctcgt cgtcacggac gagctgtgcg
    15841 atccgtcgat ccggctcctc gccggcgcat tgggactcaa tctgcgcggc ctgaaagccg
    15901 acgagcacgg gctcctgccg gaggcgtttg agaatgcatg ccaaaccgaa aaagtgaagg
    15961 cgattatctg catgccggac caccacagcc ccaccttggt ggtcatgccc gaacaacgta
    16021 ggcgcgcgat tgcggagatt gcccgcgcgc acggcgttac tctaatcgaa aatgccgtgt
    16081 accgtccctt cctcgaggct gctccgcccc ctatcgcgag cttcgcaccg gagatcagct
    16141 actactgtac gagcctttcc aaaatcatcg cgccggggct tcgcatcggc ttcctcaccg
    16201 cgccttcggg caaggccgac gagctcgtgc tcggcatggg cgcgaccagt tggatgacac
    16261 cgccgctgat cgcggaagtc gccacccaat ggatcgagga cggcaccgcc cggcgcctcg
    16321 tcgactggca gcggactgaa ctcgccgagc gaaacaggat ggcggccgag atcctcgacg
    16381 gctttacctg ctcggccttg aagagcgggc ttcacatctg gctgcagttg cccgatcggt
    16441 ggcgcgcggc aaccctcgcg caggaggcgc gagcacgcga cgtgctcgtg actcctgcgg
    16501 acgcttttgc gataggctgg gtaaacgccc ctcacgcggt ccggatcagc cttggcggtg
    16561 cgacaggccg agaagcactc gtcgccggct tacgtataat tgtcgagatc cttggccgga
    16621 aagtcgagag cagttacctc gctatttgaa tcgaactgct cgctacggtt tggcggcata
    16681 ctggaaggtt tgagcgcaga tgatatacga gctacgccat tacacggcca agcctggcaa
    16741 ggagcagaac cttgccaacc gattcagggg gcacacattg ccgtgctttg cccggatcgg
    16801 cttcaatctc gtcgatttct gggagaattg cgagagggcg ggcgatctct ggtatctgct
    16861 gcaatgggag agcgagggga tcaaggccga gctctggcag cgcctgcgca atgacgagga
    16921 atggcagcgg atcaaggccg catccgagcg gaatggcccg ttgatcggtc gcatcgagag
    16981 cgtgttgctg acgcggccgg tctgggtcac ggccaaagga gacggctaag cgctcaaatc
    17041 tcgcacccgc cgcaccacgt ttagcgatcg cccggatgtg actttattct tatgaatgtc
    17101 catctgacat tcggcgcggc gcctgggtcc agcagttggc gaccgcccgg ccggacccgc
    17161 atgaacgacc tccatcgcga cgacagttag gaagagtcta tcttgcgcta tcatcactcc
    17221 gcccggtatc ggtcccgacg ctcgcccgtc ctcgcccgca atactgtgtc aacatcccac
    17281 ccccttgcga cccaagccgg cctcgccatg ttggcccgag gcggaaacgc cgtggatgcg
    17341 gccctggcct cggcgatcac gctcgtggtc gtcgagccga ccggcaacgg tctcggctcc
    17401 gacgcctttt gcattctgtg ggatggcagc gagctgcacg ggctcaacgc atccggctgc
    17461 tcgccggccg cttggacgcc tggacgcttt gcaggcgagc cgcagatgcc gcgcagagga
    17521 tgggagagcg tgaccgttcc aggggcggtc tccgcctggg tcgatctctc gagccgcttc
    17581 ggaacgcttc ccttcgcgac cctcttcgaa cctgcgattg agtatgctga gcgcgggttc
    17641 cacgtcacgc ccgttatcgc cgagctgtgg tggcgcggcg ccgaggagct caagcatcag
    17701 cccggattcg ccgaagcctt catgccgaat ggccgcgcgc cggcggccgg agagctcttc
    17761 aggagtccgg cgctcggccg cacgctgcgc ctcattgccg agaccaaggg agaggcgttc
    17821 taccgcggcg agctcgcgga tcgcatcgcg gattttgccc gcgagcatag cgcggttctg
    17881 acacgggacg atttggcgtc ccacgagaac cattggtgcg gcaccgtcag ccagaccttc
    17941 gccgatgtca tgctgcacga gatcccgccg aatggccagg gcatcgccgc ctgcatggct
    18001 cttggaattc tgagagagct cggggtgtct gaattcgtct ccgattcggt cgatgcttac
    18061 cacttgcaga tcgaggccat gaagctggcc ttcgccgatg tccatcgtta cgtggccgat
    18121 caccgcttca tggcggaggt cacggcgggc caccttcttg atccgaccta tctcgcctcc
    18181 cgcgcgcgcc tgatcgacca caagcatgca caggagttcg gcccgggggc accgcgcgac
    18241 ggcggcaccg tctgcctcgc aaccgccgac gaacgaggca tgatggtctc ctacatccag
    18301 tcgaactatt cgggcttcgg gtccggcatc gtcgtccccg gcaccggcat cagtcttcag
    18361 aatcgcggcc acggcttctc gctcacgccc ggccatccga acgaagtcgg cccgtcgaaa
    18421 cggcccttcc acaccatcat tcccggtttc gtgacgaggg gcgacgagcc cctcatggcc
    18481 ttcggtctca tgggcgggcc catgcaggcg caaggccacg tgcagatggt cttgcggaca
    18541 caggcctggg gccaggatcc gcaaaccgcc gccgacgcgc cgcggtggcg agtaatctcg
    18601 ggactcgatg ttgcggtgga agccgctgtc ggcgagagtt tgctcgccga tctggccgcg
    18661 cgcgggcatg cggtgatctc agaggctccg gacgcgagtt tcggcttcgg cggtgcgcaa
    18721 ctcgttcaca ggatcgacgg cggctacgct gctggctccg acccgcgcaa ggacggacag
    18781 gccgctggct tctaagtggg agcctcattg gaaagtgaag cgccccactt tgagagatga
    18841 caggaccatg acgtagcggg tctttttagc taatctcttc ggcagcccag cgggacggca
    18901 ggatccttgc acacgctgcg gcgcgccgat ggcggcgccg atcggccgcc tggagggcga
    18961 tcggatcatc ctggccccgg cggctctccg gccagccatc gactcgcgag agccttgagg
    19021 cgatcggtct gctggcgctg acggcgccgg gagagcccgt acccggccca gatgaggccg
    19081 acgagggcca cccagaaggc cccgagatac cgccagtcgc cgccgctgat gagcagaacc
    19141 aggatgacga gggtgatgac cgccgcaacg acccagcaga cgatgcgggt gcggcggaac
    19201 gcagcctcgt cgctcctctc gaactcctcg gccagcttcc ggatttccaa accggcatct
    19261 cgccgggcgt cggctgttgg gtccatggtc tttgccgatc tgcctgtcgg cccgagtgta
    19321 acatttgtcg tgccgataac cagacgcgaa cgtgtcgcgt tcccgcctga gcgacagcga
    19381 tctccgcgac agcggtctcg accgtgcaga taccccggcc gtggtcgctg aggtctatac
    19441 ccgcgacacc ggagcgacgc cgccgaaagc cgcggcgcgc ctcacgaacg atgcgggcgc
    19501 gacgtcgccc cggaacggcg aagagtacat tgcggcctga cggcccgcac ccttcgggac
    19561 caccgtcatt ccgccctccg gctccgcggc tttgtcacga gaaagctggt caggtaagcg
    19621 ctgcgggcgt agcttccgct gatggcaccg atttcggacg cccgccacca gttcccgccc
    19681 gctgtcatcc agcacgccgt ctggctctat ctccggttca cgctgagcga tcgtgatgtg
    19741 gaaggcctgc tggcggaacg tgggttcgac gtgtccgacg agaccattcg gcgctgggcg
    19801 atgaaattcg ggcccgtgtt tgctcgccgg cttcggcacc atcgccatcg ccagccaccc
    19861 ggtggcacct cgccgagatg gtggtgcgat cgccgggcga agactgtctc tgtagcgggc
    19921 cgtcgatgcc gaggacgagg ttctggatat cctcgttcag cgcgggcgcg acaagaacgc
    19981 cgccgtgaag ctggtgcgca agctcctcaa gacgcagggc ttcgccccgg cggtaatcgt
    20041 gacggacaag ctgcgctcct atggattggt tgcggcggag cttaccagtc cgctcaccgg
    20101 ggacgcgggg ggaccgcacc ggatgatgcc gcggcggcac cggcccggcc gatgaaggac
    20161 ggagcccagg aaacgatcag cagcgcgacg ccggccgcgt cgatgatcgc tgcggtcggg
    20221 aacatcatca ggatcgcggc gaccaggaac gccgtccggc gccacaatcc gatggccccg
    20281 cgcaggaact ccgttccctg gatcgccatc gccaacgcca ggtttcccag cagatagctg
    20341 aacgtcgtcc aagccacgtc gaggggcggg ccctgcagca gaacttctgg aatataggca
    20401 aaggtaaacg gcatgatcgc caaggtcgtg ccgaacttga gggcggcgaa gcccgtgcgc
    20461 atggggtccg agccggcgat ggccgcgcca gcgaaggcgg agacgcagac cggcggcgtg
    20521 atgttcgaca ggcaggccgc atagaacacc gccagatgcg cgacgaccgg ggccacgccg
    20581 agattgatca atgccggagc tgcgaccacg gagaggatga tgtaggagcc ggtcgtgctg
    20641 ctccccatgc cgatgatgat cgagatgatg gtcacgagga ttacggcgaa gaacagatta
    20701 ccgccggcga attccaccag cacggccgag aacttcaggc cgaggccggc cagaacgatg
    20761 cccccgatga tgaggccgag cgtgccgatg gcggcgccgg ccgaggtgtt gctttgggca
    20821 cccccgatca acgcctggac gaccccggtt ggtcgcatgc ccgtctcgcg gccgcgccac
    20881 gacacggcga aggcggcgac tgttccccag aatgcgcaga attcgggcga gtagccgtag
    20941 aacagcaacc cgatgatgac gatcagcgga atgaagtagt gccagccctt ccgcaggacc
    21001 gcgcgcgggt ccggcacctc ctcgcgcggc agtcccgtca ggccggtccg ggcggcctcc
    21061 agatcgacca tcgccagcac gaacatgaag aagatcaccg ccggtatgac gttcatcagg
    21121 gcgatggtga ggtagctggt ctgggtcagc gttgccagga tgaagacgcc ggcgcccatg
    21181 accggcggca tgaactgccc accggtcgag gcaatcgcct cgatcgcgcc cgcgtggtgc
    21241 ggctggtagc cgacccgctt catgagcggg atcgtgaagg tgcccgtcgt caccacattg
    21301 gcgacggagg agccggagat cgagccgaac agggccgagg tcatgacggc gatcttcgcc
    21361 gggccgccgc gccagcggcc cgccagcgcg gtgccgaggt ccatgaagaa ggtgccggcg
    21421 ccgcttcgtt ccaagaacgc gccgaagatc ataaagggga acacgaaggt ggcgaaggtc
    21481 gccgccacga cgccgaacag ggcgccctgg ctcatgaagg tgaattcgag gatccgttcc
    21541 acggacatgc cgggatggct gagccggccg ggcagccatg cgccgaaata gagctgggcg
    21601 aggaacacca gggcgatcgc caccatgacg ggccccagga cccgccggct cgtttccagc
    21661 atggcgagga tggcgacggc gcccatcgca aggtcccact ggctcggcgc gctgacgcgg
    21721 aacatggcgt aggagacgta ctggttgatc cagtagccga tggacaggac cgtcgcggcc
    21781 acccagatcc agtcgaagag gctcggccga tgattcgggg accggctgcg ggccgggtag
    21841 agcagaaaga ccagcaccga ggtcagcatg agatagaggc cgcggttggt ctcggtcgag
    21901 accaggccga aacccgaggt gtagagatag aacgcggaga accccgccgc ggtgagcgag
    21961 aagatcaacg ccgggaggcc cgtaagggtc cgggactttc ccttcaccgt gaacaaccaa
    22021 tggtctttca tcacgcccct gacctcgcga gcggttggag tacggccgcg agggccgccc
    22081 ggcatcacgc cgtggcggcc ccgcggctgc gtcagtagac ctgccggctc agttcagccg
    22141 cctcagttcg gccgcctcag ttcggccact ggcccgtttc cttgtagtag gcctcggcgc
    22201 ccggatgcat cggcacgccc agcttcagga agttcgccac gtcgggcgcc atctgggccc
    22261 aggccttgtg gccttcggcg aggcgcttct tcacgtccgg ctgatagacg agctcgagga
    22321 tctccttcac ggtctccgcc ggtacccgct cgtgagccac ccaatagacc gtcgtgtagg
    22381 gcagatccac cgccgccacg cccttgtaga tcgtctcggg catggtcccc acgtgataga
    22441 acgggtaggt ctccgtgatc ttcgccatct cctcgtcggc gtagggtagg atcttcactc
    22501 ccttggtctc cgccagctcg gtcaccgcgg cggccggcgc ggaggactgg cagagggcgt
    22561 cgatctgacc attggcaagc gcccggccgg catcggcgaa ggtcatcatg ctcggctcga
    22621 tcttgtcgag caggccgagg gcacgcagga tgttggagca gttgaacacc gtgcccgacc
    22681 ctggcggccc cagcgcgacc atcttgtcct ccaggtcgga tagcgacgag atctcgctgc
    22741 cttccagcgt gacgaaatag gtcgggccct catacgcctt ggcgacgccg cgcagcatgt
    22801 cgcccttcgg catgttcttg aatggtcctt cctgcttctg cgccagatag acgtgggagc
    22861 cgtagacgat tcccatatcc agctcgccct tgatgagccg cttcgcattc tcggtcgagc
    22921 cgccggtcgt gacctgggtg acgttgatgt cgggcagctg ctccgagatg atcgtgccgg
    22981 cggcgccgac catgtagtac atgacaccgc cggggttgga gccgccgaag gcgatgtcct
    23041 gccggtcctg cgccagggcc ggagcggcaa cggccagcgc cgttgccgtt aatgtcgaga
    23101 tcgctatgcg cttcatggtt tcctccctgt tgtgatgata cgcatcggaa aactcaccgc
    23161 agcacggatc agctcgattc gcgccgcggc ggcagcccgg ccgcgcgggc ccgtgccaga
    23221 acgcgctcca tggcgagcac caccgggcgc tcgaccatct tcccgtcgag cagcagcacg
    23281 ccgtccggcg cgtccgccaa cgcccgcacc acgcgctggg cccgcgcaac ctcctcgggc
    23341 gtgggcgtgt aggtctcgtt gatgatcggc acctgggttg gatggatcgc catcttcgcc
    23401 gtgaagccga gcgccaaggc ccgccgcgcc tcgcttccgc aaccttccgg atcgccgaca
    23461 tcgacgaagg gcatgtcgat ggcggcgatg ccggcctgcg ccgcggcctg gaccaggcgc
    23521 gatcgggcga agagcagcga ctcccatgtt cccgacgccc ccagctccgc cgagagatcg
    23581 aggccaccca gcatcacagc ggtgaggcgc cgcgtggggc cagcggtcac gatggccggc
    23641 gcccgatcga tggcgcgggg cgtctcgagc agcgcgacga gggtaaggtc gagcgcggcc
    23701 tcggtcagaa gctggtcgat ccagcacagc tcctccgcgc tctcgacctt gggaatcagc
    23761 agcgtgccgc ccgacggccg tgcctcgatg acggcgagga gatcgcgcag ccccaccgcc
    23821 ttgcgcgggc tgttgatccg cagcacgcgg tcgggagaac cgccgccgcc cccatgcgcc
    23881 aaaaaggccg tcgccgccgc ccgcgcctga tccttatggg ccggcgcgac agcgtcctcg
    23941 agatcgacac agacggcatc ggcatcgctg gtgatggcct tgtcgaagcg gtccggccgc
    24001 gatccgggaa cgaacagcac gctgcgccag ccgatcccct tgcctacggt catggcttgg
    24061 cccctttacg acgccgccgg cgggtcgcgc cgcggcggca tggtcaggaa ggcatgcacg
    24121 gcgtgccaga aacggtgccg gttgatcccg agcggcgcga catgcgcctg ccccggcaag
    24181 atgacgaact gcttgtcctt gttcgacagc cgcatccaga acgcgaccag atcctcctcg
    24241 gtcgcgatac cgtcatactc gccccggacg atcaggaccg ggcaccggat cttttcaggg
    24301 tcgaccaccg gcagatgcgc gcacatgtcg agataagtcc ccgtcggcac ccggtcgccg
    24361 agcgcgagct cgagatcggc gagcttgtcc gcgacaaggg tttcggacgt gccgggcctg
    24421 tcccggttga agatcgagtg aaagaacggg cggtcgacgg ggcgggtatt gctggcccgg
    24481 aattcgtcga gcttttcgcg ccgcttgatc agcgtcgggg ccccttctcc ggtccagacg
    24541 aacgcgtcga gcaccaggcg ctccacccat tccggatgct tctcggcgaa gagggccgcc
    24601 cgcaatgcgc cggaggattg accatagaag gcgtagcggt tctgcccggt ctcccgccgg
    24661 accacctcca tgccggctcg cagatcctcg gcgcccatgg cgatgttgga attgctgccg
    24721 gttcgcgaag agcgcccata gccctcatgg tcgagcgccc atacgtcgaa cccgtactcg
    24781 gcgaacttgt ccatgaagga atagtcccgc ccctcgatct gcatgtcgaa gcccgacggc
    24841 cccgagaagg acgacccatg gacgagaaag aagacgggac gccgaacacc ttcggccgga
    24901 tcgtcgaggc gcttgcgcca catgaacaga tcgacttcgc ccttcttcgc ccagtattcc
    24961 ttgctccaca tactctgtct ttcctgaatg ccctgtcttt cctgaatgat tgggtgcgct
    25021 tacgcgaccg gattgctcag cacgccgacg ccatccacga gaatctcgac gacatcgccg
    25081 ggtttgagga acttcggcgg cttgaacccg attccgacgc ccgcgcaagt ccccgtcgcg
    25141 atcagctcgc cgggtttcag ggtcatggtg cgcgacagcg tcgcgatcag ggtcggaatg
    25201 tcgaagatca gcttcgaaac ggtcgtgtcc tggcgcaatt cgccgttcac gcgagtctgc
    25261 agacgcgagc gcgtgatgtc cgggatctcg tcacgcgtga ccacggcggg ccccatcggg
    25321 cagaacccgt cgacgctctt gccgaggaac cattggttgt gacgctgctg gagcgtgcgc
    25381 gcagtgacgt cattgatgat cgtgtaaccg aagatgtggt cggacgcctt ctccgccggg
    25441 atgtttcgcc cgccggcgcc gatgacgacg gtcaactccc cctcgtaatc ggtggaaccg
    25501 gtcggatcgt tatgcgcggg aatggcggcg tcgggaccga tcacgctgtt gggggccttc
    25561 gtgaagaaga tcggctcctc gggcaccgcg ctgcccgaag cgctcttgtt gaacccgctg
    25621 ctgtcgaact cgtgcgcatg ctcgtaataa ttcttgccga cgcagatgag gttgcggatc
    25681 ggccgcggga acggcgcttc cagccggacc tccgacaggg ggatcgccgc gccttctccg
    25741 ccccgctcga ccgccgcgcg ggcgcgggtc agcgcgtccg agcccgcctc gatgaaggcg
    25801 tacatatccc cggggagcgc cggatcctgc gcctgcaaat cgatgatcct gtcatcgccg
    25861 gcgtgcgctc cgagacgcag atgcccgttt cgagaaaacg tgaccagctt cacgcgtcca
    25921 tcctcctttt cttctaaagc ccgacccggc gcatttgcgt ctcgggatat cgcgtgcccc
    25981 gaacggttcc agaagcgaag gccgcatcca ggctcgcgat ctccgcctcg gccagtctca
    26041 ggtcgagcgc cgcgaggctg tccatcagcg tccgccgccg cgagcagccg ggaatcggaa
    26101 cgacgtcatc gccgcgcgcc aggagccaag ccaacgcgat cgccgccggg gagacgccgt
    26161 gacgaccggc cagatccgcg acgcgatcga cgagcgtcag gttatgtctc agattctcgc
    26221 cctggaaacg cggcagttgt cggcgccgat cgttctccgc caggtcgccc tcgccgcgaa
    26281 cgcggcccgc cagcaggccc cgcccgatcg gcgcataggc aacgaagccg atcccgagct
    26341 cacggcaggt gggcaggatc tccgcttcca cgtcgcgcgt gacgagcgag tactccgtct
    26401 gcaacgccgc caggggatgc acggcctgtg cgcgtcggag agtctgggct cccgcctcgg
    26461 acaagccgat ccagcggacc tttccctgct ggacgagacg ggccatcgcg cccacggtgt
    26521 cttcgatcgg caccgacggg tccacgcggt ggagatagta gaggtcgatg gtttcgaccc
    26581 cgagccgctt cagggacgcc tcgcaggcct gggcgacata ctcgggacgc ccgttgacgc
    26641 tccgcgtgcc gtcggggcgg cggatattgc cgaacttcgt ggccaggacc acccggtcac
    26701 gacgtcccgc aacggtgcgg ccgactagct cctcgttgcg cccgccgcca taggcgtcgg
    26761 cggtgtcgat caggataaca ccggcatcga tggcggcatg gatcgtccgc accgcctcct
    26821 ccggatcggg gggatcatag atcggcgtca tccccatgca gccgaggccg agcgccgaga
    26881 caaccggtcc agcggacccc aggcatcgtt cacgcatctg gcctccgtga accaattcgc
    26941 caccaagcgg gtttcatgca acgattgtcc cagtcaggga ggcgtgccac cctgcccccc
    27001 gaaatgcgac cgagaggacg agtccaatgg cgtttggcgg acgcgacgcg aaggtgctgg
    27061 ccgtaatcgg atcgggccat gcgatgagcc atttctatct gctggccttg ccgccgctgt
    27121 tcccgctgat ccgggacgat ctggacgtca gctacgcggc gctcggcctg ctcctgaccg
    27181 tcctgaacgt tgcgaccggc ctgttccagg tccctgccgg catcctcgtc gatcgtctcg
    27241 gcgcccgcac catcctggtg gccggtctga gcttgatggc cgccgccatg gccggcatcg
    27301 ggctcgccga cagctattgg accatgatcc cgctgatcgt cgccgccggc atcggcaaca
    27361 gcgttttcca ccctgccgac tacgcgatcc tggcgcgctc ggtcgatcag tcgcgcctcg
    27421 gccgcgcgtt cagcattcac accttcacgg gcaatatcgg cttcgccctg gcgccgcccg
    27481 tcatggtcgc gctgacggcg gccctgggat ggcgcgtcgc cttgatggcg gcgggcgcga
    27541 tctctcttct ggtccttatc ggattcctcg tctggggcgg cgtactcgcg gacgatgcgc
    27601 ccgacggggc cgcacaagcg aggcaggatc gcagcgtcga tcttcgcatt ctgctcgaca
    27661 gccgcattct tattctgttc ctgttcttcc tgtcggtcgc gatggtgacg agcggcgttc
    27721 aatcgttctc cgtcgccgcg ctgcaaggct tccaaggcct tgacttcacg gccgccaaca
    27781 ccgtcctgac cggctttctc gtagcatccg cggcaggcat cctgatcggc ggattcgtcg
    27841 ccgatcgcac gaagcgtcac ggcaccgtgg ccgtggccgc catgctgggc gcggccgccc
    27901 tgttcctgct gagcggcctc gccgcgctgc ccttcgctgt cctgttcgtc gccttcgtcg
    27961 ccatcggcct gctgcagggc gtcattcgac cggcacgcga catgatggtg aaggcggcca
    28021 cgccggaagg ggccacggga cgcgttttcg ccttcgtgag caccggtctc aatgtcggcg
    28081 gcgcgatcac gccgttgctg ttcggtctcg tgctcgatgc cggcgaaccc cggctcgtgt
    28141 tcctgctgtt gtccgtgttc gtgctcctgg gcatcggcac gattggcgtc gttgggcgta
    28201 agcggcccgc agcctccagc gtcccggcgg aatgagggtg accggccagg catcgctcag
    28261 ccctcgaact cgagaatatc gaagccgcgg ttgcggtcga tcaggtagac gaggccgcgt
    28321 tcgtcgacct caacgtcgtt ggtctgcggg cttgggttgc cgccgaccgg ctccgggatg
    28381 aagaaaccgg tctcggccgg ccgagcggga tcagcgatgt cgaccacgcg gaggccgccg
    28441 gaaaaccacg cgcagaacac caaattgccg tcgatatgct cctggaactg gtgcatgccg
    28501 aagcggcccg gcgcgcggct ccagggggag tccaactcgc tgacgtgaaa ggtcgcgacc
    28561 ggcgcgatcg cgctcaggtc ggtgacgtcg aacagccaca ggccggcatg gggctggccg
    28621 cgacggtgct cgtgctcctc gtcggccacc accgcgatcc gcctgccctt cgggtgcccc
    28681 gca
//
LOCUS       DASVDU010000246        13425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1169303, whole genome shotgun sequence.
ACCESSION   DASVDU010000246 DASVDU010000000
VERSION     DASVDU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13425
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169303"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF226_16100"
     CDS             complement(<1..345)
                     /locus_tag="VF226_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX7075561.1"
                     /translation="MGIALAGLSAICAVTAYGQIDTRSQYGGTTAAVVDDKGNLRVPS
                     DYRTTYQVLGSWAVAKDDGPGSKQLHVVYASPGTVAAYRKDGHFPDGAVLVKEVFNTT
                     TKDMTTGTVSSAG"
     gene            complement(410..1006)
                     /locus_tag="VF226_16105"
     CDS             complement(410..1006)
                     /locus_tag="VF226_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX7075562.1"
                     /translation="MRITPLIAAAVSSAVAITAAFAQSESNDGSAPTTKRYLPEYTES
                     GDLILPKNWRHWVFVGSPLTPNALNDGKANFPEFHNVYIEPGSYEIYKKTGEFPEGTI
                     FFKELQLTLKGENPDGSRTEPSGRGYFPGPFNGADVTVKDTKRYAATGGWGYYNFNHH
                     EPKAPTAKLQPKSECAFCHMASAKKDDVWTQFYPLLDK"
     gene            1434..2171
                     /locus_tag="VF226_16110"
     CDS             1434..2171
                     /locus_tag="VF226_16110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7075563.1"
                     /translation="MPISATLEFGTSGIETDLTIVAGLGIVGIAVYAFWERRSKHPMT
                     PPRLVENRAFLGLNVATLMVYTGLSIMFFLLPFDLVDRRVLPLTDAGVAFPPFALGLG
                     LLSRVFGRVADKNGARAMLIAGPAGAALAYVWMALGQKESSMLGVIVPMTLLGPSFAV
                     RVAPLTASVMSSVDQTDKGLASGINNAVSRIAQLAGVALAAGVASFESGYAVGLAVAA
                     ATSIGGAFTAAITPTPVAAKAGGSGRA"
     gene            2177..3052
                     /locus_tag="VF226_16115"
     CDS             2177..3052
                     /locus_tag="VF226_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7075564.1"
                     /translation="MEFNQIRYFLHLADTLNFTEAAMRSGVSQPTLTRAIQRLEQELG
                     GTLVYRDGKDSRLTALGRDIRSEFAAIAEREQRVRAISLNRVRGHRETLTLGVANTIA
                     PALITGFVRHALRQMPMLELVLQPITRDEGMERLLGGQIDGCFCSDPMAGNTKIATVE
                     MFHERLLLAMAEGHTLASHASVSLSDLSEQPYLDRLQCEFRTRVQNHLHEREIIMIPR
                     LRSEREDLIQQAVAEGAGVCMLPERSAIVGGLTLRPVETLNLSRTIAFQSISGSGTAA
                     ALRKLRMLIESHVWS"
     gene            3259..3705
                     /gene="msrB"
                     /locus_tag="VF226_16120"
     CDS             3259..3705
                     /gene="msrB"
                     /locus_tag="VF226_16120"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7075565.1"
                     /translation="MAAYKKTEAAIAKLSPEEYRVTQQSGTERPGSGALLFNKEPGIY
                     VDIVSGEPLFASSDKYESGCGWPSFTKPIEPANVNELRDTSHGMIRTEVRSTHGDSHL
                     GHVFTDGPSDRGGLRYCINSASLRFVPRDEMVEQGYGEYLNQVEDI"
     gene            3705..4217
                     /gene="msrA"
                     /locus_tag="VF226_16125"
     CDS             3705..4217
                     /gene="msrA"
                     /locus_tag="VF226_16125"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7075566.1"
                     /translation="MTTERAVLAGGCFWGMQDLIRKLPGVISTRVGYTGGDVPNATYR
                     NHGTHAEAIEIIFDPSQTTYRRLLEFFFQIHDPTTLNRQGNDIGMSYRSAIYFISDQQ
                     KAVALDTIADVDASGLWPGKVVTEVGPVGDFWEAEPEHQDYLERIPHGYTCHFPRPNW
                     VLPHRAGAAA"
     gene            4382..4915
                     /locus_tag="VF226_16130"
     CDS             4382..4915
                     /locus_tag="VF226_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEX7075567.1"
                     /translation="MEAGIVGLPAPPLEHVRWINENGDERSPLTLAELGQSFTILYFF
                     QDWCSGCHAHGFPTFVTLAEGLRDKGVGLAAIQTVFEGSEVNTFDRLRENQRRYGLRV
                     PFGHAVADSASGDAVPAIMEAYRSGGTPWFVVIAPNGRVVYDGFRLDAKDLVQALRPL
                     APMPTPVVARGVGQKTE"
     gene            complement(5491..6000)
                     /locus_tag="VF226_16135"
     CDS             complement(5491..6000)
                     /locus_tag="VF226_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX7075568.1"
                     /translation="MQIPIIERRRIEAQLLKIIHDELVTRLGEAAAEEIIAESVRKSA
                     IEQGRQFASAEPDGTSMSTFVKLYDLWTMNDALEIDVLHQDAERFAFNVTRCRYAEMY
                     REMGLGQIGHLLSCNRDGTFCQGYDPNIKLERSQTIMRGASHCDFRYRYEHQPPAEVR
                     DATAEDGDQ"
     gene            6075..6311
                     /locus_tag="VF226_16140"
     CDS             6075..6311
                     /locus_tag="VF226_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075569.1"
                     /translation="MARAARSLVEFQTAFPDDAACAALPFRRRWPHGFVCPGRDARKR
                     TVRLRSAAHLMAAHANGISARQLRSPLHVTYKTA"
     gene            complement(6387..7904)
                     /locus_tag="VF226_16145"
     CDS             complement(6387..7904)
                     /locus_tag="VF226_16145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX7075570.1"
                     /translation="MSNAGNLSSVLSGWPEPERTALVDLSAGRVREFSFGALSSLSDS
                     VARGLHSRFGSGRFRVGIVARNSAEFVALYFGTMRAGGVAVPISWKLPPEILEYVLTD
                     ASVDAVFSDGSCELPDSASAVRIDRDWTSFLDPGCFEPAPVSRDSLAQILYTSGSTGR
                     PKGVPLTHGGQFWAVEKAASLITDPSSYRVLVAAPLFHMNALFNVKRTLYCRSTVVLL
                     PHFTAQDYERAIEEFHCDWVTCVPAMMALLARHLGDRVLPPRFAAVRRVFMSSSSFSP
                     QLLATVQRMFPNAHIANSYGTTEAGPHVFQGHPNGLPTPPMSCGHPYSPDHVRLVGED
                     ERVIQGEGQGVLEMNTPATASGYLNLPETSAQLFRDGWYNSRDIMRRDANGFYYFMGR
                     VDDMFTCGGENIYPIEVEQILERHPDIEQAVVVPVPDEIKQSLPVAFVVPRSGANLDE
                     TPLKEWFLDRAPGFMCPRRIGFVKEIPLAQTNKVDRGHLSEMARKLMARSPQPQS"
     gene            complement(8134..9081)
                     /locus_tag="VF226_16150"
     CDS             complement(8134..9081)
                     /locus_tag="VF226_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7075571.1"
                     /translation="MTRPLSDDLRRRVIAAVDGVLSCRTAAKRFRVAASTAIKWVDRH
                     RQLGHVRPYPQGGDRRSHRLEAYAEEILSLIDARLDITLEEMATHLDEAHGVVVSQST
                     IWRLLDRRGITFKKTAHASEQQRADVLRLRRAWFEGQIDLEPERLVFIDETGASTKMA
                     RRYGCAPRGERCRAPVPHGHWKTTTFVGALRLEGMTAPMIVDGPMHGAAFIAYVEQVL
                     VPTLKPGEIVVMDNLPAHRPIAVRKAIETAGAELRFLPPYSPDFSPIEMAFAKLKAFL
                     KKIAARTVDALWSAIAEAIDLFTPSEGENYFAAAGYDRD"
     gene            complement(9418..9558)
                     /locus_tag="VF226_16155"
     CDS             complement(9418..9558)
                     /locus_tag="VF226_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075572.1"
                     /translation="MARRQRHKPERIIGMLREIGAGGRPDDWSGMPRVGITEQMYYFA
                     GA"
     gene            9862..11361
                     /locus_tag="VF226_16160"
     CDS             9862..11361
                     /locus_tag="VF226_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7075573.1"
                     /translation="MSPLTLAPEIADTRLYQSAVERLREAQVVLPTLTQLADPSLIPD
                     GIAAGLPSIDLDEPAAANLFRVHWYNDADRTGRTEVPGYLELPEALTGSKARIVVALG
                     RRFPMIGAHKVLAAYGCLMPRLVTGRFDPVNHKAIWPSTGNYCRGGVAISRILGCRGV
                     AVLPAGMSQERFDWLHRWIARPEDVIRTPGSESNVKEIYDKCAELSRDPSNVILNQFS
                     EFGNYLVHYHCTGRALESVFQDLRRTDPELRLAAFVSATGSAGTIAAGDYLKSAYGTK
                     IVALEATECPTMLFNGYGEHNIQGIGDKHIPLIHNVMNTDLVVGISDRSTDALNLLFN
                     TEIGRAYLEKRQHIDAELLRHLDGLGISGIANVLGAIKVAKHLELTESDLLVTVATDS
                     AALYESEREGYMARTYPEGFDEVNAAEIFAGHLQALSDDHLIELTHVDRRRIFNLGYY
                     TWVEQQGVSVEDFDRRRDPAFWRELQESIPAWDRLVEDFNERTGVAGRT"
     gene            11480..12364
                     /locus_tag="VF226_16165"
     CDS             11480..12364
                     /locus_tag="VF226_16165"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7075574.1"
                     /translation="MSLEEAASLVKDGDQVGIGGSTLSRTPMAMIWALIRAKRKNLSC
                     SRSIVSSEGDLLFASGASDHILTSWFSQGIVWGVSKVMRHYTESRKAWFEEWSHMAVG
                     LRFRAGAMGVPFLPMRSMLGSDVVRRIEDVHEVDCPFTGEKLVLVPALNPDVALIHVQ
                     RCDAYGNAQMDGLQFMDIDLAMAATRVILTTERIVSNDQIRRAPDQTKIPFFAVDAVV
                     EVPFGCAPHECYGVYEPLFKHMDYYTALVNKDPEAGTRAYLDRYVYGPKSWTEYLELL
                     GLEELLDAGRRGRSIYDD"
     gene            12357..13127
                     /locus_tag="VF226_16170"
     CDS             12357..13127
                     /locus_tag="VF226_16170"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7075575.1"
                     /translation="MTSRRSYTASELLAVMSARLLKDGQVVFAGVGIPLLAATLAQRL
                     HGPGLTILFEGGTIGAFVEPGKLPPSTNEQRCTRRANMVLGSTDVLILLQRGYVDVGF
                     MGGAQIDQYGNLNSSYIGDADDPRIRLPGTGGGNDISSLTQMIVAMKHEKRRFVEKVD
                     FITSPGFLHGRTSRKDSGLLAGGMYRVVTDLAILGFDDETKRMKVIALHPGVEVDQVQ
                     ENTGFELAIGSDVGATEPPTEQELAVLRLLDPERLYIA"
     gene            13179..>13425
                     /locus_tag="VF226_16175"
     CDS             13179..>13425
                     /locus_tag="VF226_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7075576.1"
                     /translation="MAGLSNTFGIAMRNFTAYPQLPDARGLIEYGVKMEQMGFDSLWV
                     WDHILLGVDPNFPIIDSLSLLTAVAARTKKIRLGTGIL"
BASE COUNT         2573 a         4131 c         4084 g         2637 t
ORIGIN      
        1 gccggcactg cttacggttc cggttgtcat atctttagta gtcgtattga acacttcctt
       61 caccagaacg gcgccgtcgg gaaagtgtcc gtccttgcga tacgcggcga cggttcccgg
      121 cgatgcatag accacgtgca gctgctttga gcccggaccg tcgtctttag ccacggccca
      181 gctgcccagc acctgataag tggtccgata gtcggaaggc acgcgcagat ttcccttgtc
      241 gtcgacaacg gctgcagtcg ttccgccgta ctgtgagcgc gtatcgatct gcccgtaggc
      301 agtgacggcg cagattgcgg acaatcctgc cagggcgatg cccaagacac caaggattct
      361 catagcgctg tcccttgtgt tggtgaacca gtagcgttcg ctgccgattt cacttgtcca
      421 gcagcgggta gaattgggtc cagacgtcgt ctttcttcgc gctcgccatg tggcagaacg
      481 cgcactcgga tttgggctgc agcttggccg tcggcgcctt cggctcgtgg tgattgaagt
      541 tgtagtaacc ccagcctccg gtggcggcat agcgtttggt atccttgacc gtaacgtcag
      601 cgccattgaa tggcccaggg aaatagcctc tcccggaggg ttcagtgcgc gatccgtccg
      661 gattctctcc tttcagcgtc aactgcagct ctttgaagaa aatcgttccc tctgggaact
      721 cacctgtctt cttgtagatt tcgtatgagc ctggttcgat gtagacgttg tgaaattcag
      781 gaaaattggc cttcccgtcg ttgagcgcgt tgggagtgag tggcgatccg acgaacaccc
      841 aatggcgcca attcttcggc aggatcagat cgcccgattc ggtatactcg gggagatagc
      901 gcttcgtcgt cggtgcgctg ccgtcattgg attcggactg ggcgaatgca gccgttatag
      961 caaccgcgct ggaaaccgct gcggcgatca gtggggtaat acgcatgctt tttctccacg
     1021 acatcgagta atatccgtcc caagtgccct cggcatgaat cgatgagatc tgcgacaatg
     1081 atgctcagtt gtggcaccgc gggaggaagt tttccaatgc actgtgggta tagtttccat
     1141 agcggcgggg aatgctgcga cggcattgat tgccgataca gcgactgaac gcggcctaga
     1201 cgtctccgca gaactaagaa cgtcggtttc gaggagcgat gcgggtcgcg gcattcctcg
     1261 ctacggtgtc acgccgcgcg gcgagcggta ataacgatcg tctatcgact ctatggcgag
     1321 aggtgaattc cctcgccggc gttgctaggt cactctccaa agcatggtca tacttcaagg
     1381 cagtactgcc tccagcatcg gcgggcggca gcacgctcgg cctatcgtgg cctatgccta
     1441 tctctgccac cctcgaattc ggcacgtccg gcatcgagac tgaccttacg atcgtagccg
     1501 ggctcggcat tgtcggaatc gccgtctatg cgttctggga acgcagaagc aaacacccga
     1561 tgacgccacc acgcctggtg gagaaccgcg ccttcctcgg gctgaacgtc gcgaccctga
     1621 tggtctacac ggggctttcg atcatgttct tcctgctacc ctttgatctc gtcgaccgcc
     1681 gcgtcctgcc attgaccgat gccggggtgg cattcccgcc cttcgcgctc ggcctcggac
     1741 tcctgtcgag ggttttcggg agggtggcgg acaagaatgg cgcgcgggcc atgctcatcg
     1801 cgggaccggc aggtgcggca ctcgcctatg tctggatggc gctcggtcag aaagaatcct
     1861 cgatgctcgg cgtgatcgta cccatgacgc tgcttggccc gtccttcgcc gtgcgtgtgg
     1921 ccccgctcac cgcgtccgtc atgtcgagcg ttgaccagac ggacaaaggg ctcgcctccg
     1981 gcatcaacaa tgcggtaagc cggattgcgc agcttgccgg ggtggcactg gccgccggcg
     2041 tcgcgtcttt cgagtccggc tacgcagtcg gcctcgccgt ggccgctgcg acctcgatcg
     2101 gcggcgcctt tacagccgca ataaccccga cgccggtcgc ggcgaaagcg ggcggctcgg
     2161 gaagggctta gaaagcatgg aattcaacca gattcgatat ttcctgcacc tggcagacac
     2221 ccttaacttc accgaagcgg ccatgcggag cggcgtgtcc cagcccacgc tcacccgcgc
     2281 catacagcgg ctcgaacaag agttgggcgg aacgttggtc tatcgcgatg ggaaagatag
     2341 tcgactaacc gcactcggcc gcgacatccg atccgaattc gctgccatcg cggagcgtga
     2401 acagcgggtt cgggcgatat ccctcaatcg ggttcgcggc caccgcgaga ctttgacgct
     2461 gggcgtcgca aacaccatcg cgccggcgct gattaccggg ttcgtcaggc acgcccttcg
     2521 ccagatgccg atgctcgaac tggttctcca gccgatcaca cgcgacgagg gaatggagcg
     2581 cctcctcggc ggtcaaattg atggatgctt ctgctcggat ccaatggccg gaaacaccaa
     2641 gatcgccacc gtcgaaatgt tccatgagag actgttgctg gcgatggcgg aagggcacac
     2701 cttggcaagc cacgcctcgg tttcgctttc ggacctgtcg gagcaaccgt acctcgatcg
     2761 gctccaatgc gaattccgca cgcgcgtgca gaaccatctg cacgagcgtg agattatcat
     2821 gatcccgcgg ctcagatccg aacgagagga tttgattcaa caggccgtgg cggagggcgc
     2881 cggcgtgtgc atgctacctg agcgctcggc aatcgtgggc ggtctgacgt tacgcccggt
     2941 cgaaacgctg aacttatcac gtacgatcgc attccaatcg atttcgggat cgggaacggc
     3001 agcggccttg cggaagcttc gcatgctgat cgagagtcat gtgtggtcgt gaattctgtg
     3061 gggtgccggc gcttatggtg cgtcgcaggc aatcgcgtgg ccaaagcgaa atccgcaacc
     3121 aagctagggg cggcacgatc gcgcatgccg gtgggtaaca tcgaaacttg ggtaacatcg
     3181 aaactatagt cgcggcgtat tggacacctt cggatcgagt gtccatcttc aatggcagga
     3241 attcgaagga gtttgaccat ggctgcctac aagaagacag aagctgcgat cgctaagctg
     3301 tcgcctgagg aatatcgggt gacgcagcag agtgggaccg aacgcccagg aagcggcgcc
     3361 cttctcttca acaaagaacc aggcatttat gtcgatatcg tctcaggtga accgctgttc
     3421 gcatcgtcgg acaaatatga gtcgggctgc ggatggccga gctttaccaa gccgatcgag
     3481 cctgcaaacg tgaatgaact gcgcgacacc agccatggca tgatccggac ggaagtgcgt
     3541 tcgacgcatg gcgatagcca tctcggccac gttttcactg acggaccgag cgatcgcggc
     3601 ggcctgcgct actgcatcaa ttcggcttcg ctgcgcttcg tgccgcgcga cgagatggtg
     3661 gagcagggct acggtgaata tctcaaccag gtggaggaca tctaatgacg accgaacgtg
     3721 ctgttctcgc cggtggctgc ttttggggca tgcaggacct catccgcaag ctgcccggtg
     3781 tgatctcgac ccgggtcggg tatacaggtg gcgacgtgcc gaatgcgacc tatcgcaatc
     3841 acggcaccca tgccgaggcg atcgaaatca tcttcgaccc ctcgcagacc acctatcgcc
     3901 gattgctcga gttctttttc cagatccatg atccgacgac gctcaaccgt caggggaacg
     3961 acattggcat gagctaccgc tcggcgatct atttcatcag cgatcagcag aaagccgttg
     4021 cgctcgatac gatcgcagac gttgacgcgt cgggattatg gcctggcaag gtcgtcaccg
     4081 aagtcgggcc agtcggtgat ttctgggagg ccgagcccga gcaccaggac tatctcgaac
     4141 gaattccgca cggatatacc tgccattttc cacgtccgaa ctgggtgttg ccgcataggg
     4201 ccggtgctgc cgcctagagt gcgatccgat caggttgggt caacctgatc ggtgaatcgc
     4261 cctctacttt aaatgtttag agactgattc accgatcaca tcggttcgat gtgatcggat
     4321 caggctctag caaacccgcg gctcgcggca cgacgccgcg agcccgctga tggaggattt
     4381 catggaggcg ggaattgtgg gtctgccggc ccctccgctc gagcatgtcc ggtggatcaa
     4441 tgaaaatggc gacgagcgta gccccttgac cctggccgag ttgggacaga gtttcactat
     4501 actgtacttc tttcaggatt ggtgttcggg ctgccatgca catggctttc ccacattcgt
     4561 gacacttgcc gagggacttc gcgataaggg agttggcttg gcagccatcc agaccgtgtt
     4621 cgagggttcg gaagtcaaca ccttcgatcg tctgcgggaa aatcagcgac gatatggcct
     4681 gcgtgttccc tttgggcatg ccgtcgctga ctcggcctca ggagacgctg tgcccgcaat
     4741 catggaggca taccgctccg gcggaacccc ctggttcgtt gtgatcgcgc ccaatggccg
     4801 cgtcgtgtac gacgggtttc ggctcgacgc gaaggacctc gtccaggcac ttcggccgct
     4861 cgccccgatg cccacgccgg tggtcgcaag aggagtcggc cagaaaaccg aatgaaaaat
     4921 aaaatgagcg attgtccgag atcgagcatc gccattgcag ggcgcaccat accttccagc
     4981 ggttcgcgtt cggggcggcg cgaacgaggg cgcgaatgcc aaccaagaca gccaacaggt
     5041 gccgggcaag gtcatcagcc acgccgggca ttacggcgcc gcccttgccc gggtccgccc
     5101 ctgttcatcg aagacgtccg ccatcggatc tgtctgtgct gccgtttgcc ggtacgggct
     5161 ttggccactc gtctggatcg gcaacgggat gatcgaaccg gcctcgttca gtccgttcca
     5221 tggtccctcg cttccggagt cccgttcacg gcgtcggttc cgtggaccat cgatccggtc
     5281 ggcatgccgt ggcccgccag cggcgatgag tccgggtcag aggttgccga ggcagtaacg
     5341 gaacctttat cgcaacaccc gcgaaacccg ccacggctgt tgacttgata aaaggatgaa
     5401 agatcgacaa acttggtcca tggggggcct ccacttagac tgacttcggc ggcgcggcga
     5461 acgcgagtgg ggagctgacc cttcaccttc tcattgatcc ccgtcctccg ccgtcgcatc
     5521 gcggacctcc gccggcggct gatgctcgta gcgatagcgg aaatcacagt ggctggcgcc
     5581 gcgcatgatg gtttgcgagc gctccagctt gatgttcgga tcatagcctt gacagaatgt
     5641 cccgtcgcgg ttgcacgaca gcagatgccc gatctgcccc agccccatct cccgatacat
     5701 ctcggcgtac cggcagcgcg tgacgttgaa ggcgaaccgc tccgcgtcct gatgcagcac
     5761 gtcgatttcg agggcgtcgt tcatggtcca gaggtcgtag agctttacga acgtgctcat
     5821 cgatgttccg tcgggttcgg cagacgcgaa ttggcggcct tgctcgatgg ccgacttccg
     5881 gaccgactcc gcgatgattt cttcggctgc cgcctcaccg agccgcgtta cgagctcatc
     5941 atggatgatc ttcaggagct gcgcctcgat gcggcgccgt tcgatgatcg ggatttgcat
     6001 tgcttcacct catgccattc ttcggtctca tccaataatg ctcggtgctc ggcggtagcg
     6061 aaggtgccgc gccgatggcc agagccgcca gatcccttgt cgagttccag acagcgttcc
     6121 cggacgatgc cgcctgcgcc gcgcttccgt tccggcgccg ctggccgcac gggttcgtct
     6181 gcccgggccg cgatgctcgc aagcgcaccg tccgtctaag gagcgccgcc cacctcatgg
     6241 cggctcacgc caacggcatc tcggcgcgcc agctccggtc cccgttgcac gtcacctaca
     6301 agaccgcctg actgctcatg cagaagtcgc ggcggaccat ggtcgatcca ggccgcgagc
     6361 catgacgttt ttctcccttg aatcacttat gattgaggct gcggtgaacg agccatcagc
     6421 ttgcgggcca tctccgacaa atggccgcgg tcgaccttgt tggtttgagc cagcggaatt
     6481 tcttttacga agccgattcg ccgcggacac atgaagcctg gcgctcgatc aagaaaccat
     6541 tccttgagcg gcgtttcgtc caaattcgcg ccgctgcgcg gaacgacgaa tgcaacgggg
     6601 aggctttgct tgatttcgtc gggaaccgga acgacgaccg cctgctcgat atcggggtga
     6661 cgctccagaa tctgctcgac ctcgatagga tagatgttct cgccgccgca agtgaacatg
     6721 tcatcgacgc gccccatgaa gtaatagaac ccattcgcat cccgccgcat gatgtctcgg
     6781 ctgttatacc agccatcacg gaagagctgc gcggatgttt cgggcaagtt gagatagccg
     6841 gacgccgtag cgggcgtatt catctcgagc acgccctgtc cttcgccctg gatcaccctt
     6901 tcatcctcgc cgacaagcct gacgtggtcg ggcgagtagg gatggccgca tgacatgggc
     6961 ggtgtgggaa gaccgttcgg atgtccctga aagacatgcg gccctgcctc ggtcgtcccg
     7021 taggagttcg cgatgtgagc attcggaaac attcgttgaa cggtcgccaa aagctgtgga
     7081 ctgaacgagg acgaactcat gaagacacgc ctgaccgcgg cgaagcgcgg cggcagcaca
     7141 cgatcaccga ggtgacgtgc gagaagggcc atcatggctg gcacacaggt gacccagtca
     7201 cagtgaaact cctcgatggc gcgctcatag tcttgcgcgg tgaaatgcgg cagcagcact
     7261 accgttgatc ggcagtagag tgtccgcttc acgttgaaca atgcgttcat gtgaaacagc
     7321 ggcgccgcca ccagcacacg ataggagctg gggtccgtga tcagcgaagc cgccttctcc
     7381 accgcccaga attgaccgcc gtgcgtcagc ggaacgcctt tcgggcgccc tgtcgagccg
     7441 gatgtgtaga gtatctgggc caggctgtcg cgcgagaccg gggccggctc gaagcatcca
     7501 ggatccaaga aactggtcca gtctcgatcg attcggaccg cagatgcgga atccggcaac
     7561 tcgcaagagc catccgagaa caccgcatcg acgcttgcgt cggtcagaac gtactcgagg
     7621 atttccggcg gaagtttcca gctgatgggc accgcaacgc cgccggcgcg catggtcccg
     7681 aagtaaagcg cgacaaattc ggcactgttt cgtgctacga tgccgacgcg gaatctgcct
     7741 gagccgaacc ggctatgtag gcccctagca accgaatcgg acaggcttga cagcgcccca
     7801 aagctaaatt cgcgcacgcg accggcggac aagtccacca acgccgttcg ctccggttcc
     7861 ggccaaccgc tgagcaccga gctgagattt ccagcgttcg acatcggcta tcgctcgagg
     7921 acactttttg atcgctgcac tggaggatct aaatgcagac tgacgctagg cttccagcct
     7981 cgtcaactgt aaaacaggcg gatattctgc aaaatgactg aacgtgcagc tgcattttgc
     8041 cgcgcgggcg tatttttgta tatattttca tctagtagta gagaatccgc gggcgatggc
     8101 agagcagatc gacggatcta gagcattttc cgatcagtcg cggtcatagc cggcagcggc
     8161 gaagtagttt tcgccctctg agggggtgaa gaggtcaatg gcttcggcga tggcgctcca
     8221 caaggcgtcg acggtccttg cggcaatctt cttgaggaag gccttgagct tggcgaaggc
     8281 catttcgatc gggctgaagt cggggctgta gggcggaagg aaccggagct cggcgccggc
     8341 cgtctcgatc gctttcctga cagcgatcgg cctgtgggcc ggcagattgt ccataacgac
     8401 gatttcgccc ggcttgagcg tcgggaccag aacctgttcg acataggcga tgaaggccgc
     8461 tccatgcatc gggccgtcca cgatcatggg tgcggtcatg ccttcaagcc ggagcgcgcc
     8521 gacgaaagtc gtcgtcttcc agtggccgtg cggcacaggc gcacgacaac gctcgcctct
     8581 cggcgcgcag ccatagcggc gggccatctt ggtcgaagcg ccggtttcat cgatgaagac
     8641 caggcgctcg ggctccagat caatctggcc ctcgaaccaa gcccgcctaa ggcggagcac
     8701 gtcagcccgc tgctgctcgg atgcgtgggc ggtttttttg aaggttatgc cccggcggtc
     8761 aagcaagcgc cagatcgtgc tctgagagac gacgacgccg tgggcctcat cgagatgcgt
     8821 cgccatctct tccaaggtga tgtccagccg ggcgtcaatc agtgacagga tctcttcggc
     8881 ataggcttcg agccggtgcg aacggcggtc gcctccctgt gggtacggcc gaacgtgtcc
     8941 gagctgccga tgccggtcta cccatttgat cgccgtcgag gctgctaccc tgaagcgctt
     9001 cgccgccgtt cggcaggaca gcacgccatc caccgccgca atcacacgcc gtctcagatc
     9061 gtccgaaaga ggtcgcgtca tgcaagctgg cctccttcac cagcctgcat cgtgaatcac
     9121 aattcgctcc tcgaaggaac ccctcgcgat tccgtcagat cggaaaatcc tctagtaggt
     9181 cgcacgtgct gccggatggc gggctggggg gcaataccgt tgcacggccg catggacgcg
     9241 cgtgacgaac ggttgcgtgc ggcgatccgt ggggcatccc cgacgatcac cacgcaccac
     9301 gcggccctgc gggcggacct gtgcaatgtc gataacgtgg cggcggtcgg gcggggttca
     9361 tcggtttgct gacgttgccc ttcggatcgc aaggtcgcct ctttgaggat cagctcgtca
     9421 agcaccagca aaatagtaca tctgttcggt gatgcccact cgcggcatgc ctgaccaatc
     9481 gtctggccgc ccgccagcgc cgatctcccg aagcatgccg atgatccgtt ccggcttgtg
     9541 cctctgcctc cttgccattt ctgcctcctc ctgatggccg cctctaacac agagcccgga
     9601 ccggcttcag gggaacaggt caccggcagg ccggcaaacc gttagatgca cctaccaccg
     9661 cgggtggggt gtctccgagc agactcttgc gcaaagcaga atacgtgtat atggtaccta
     9721 cattagtaca atatacagat acggcgttga gcgaggagtg acgattccga cctcgactcg
     9781 gaacggttgc cctcgttcct tgtcttgccg atctcggcgg cgaaacggat gcccaaatgg
     9841 gaaacacagc gaaagggagc catgagccct ctcaccctag cgccggagat tgccgatacc
     9901 agactttatc agagcgccgt cgaacgcttg cgcgaggcac aggtcgtcct gccgaccttg
     9961 acccagcttg ccgatccgtc gctgatccct gacgggatcg ccgccgggtt gccctcgatc
    10021 gatctggacg aaccggcggc cgccaatctc ttccgggtcc attggtacaa tgatgcggat
    10081 cggaccgggc gcacggaagt ccccggctat ctggaacttc cggaagctct gaccggctcc
    10141 aaggcacgaa tcgtcgtggc gctgggtcgc cgctttccga tgatcggcgc ccataaggtg
    10201 ctggcggcct atggctgcct gatgccgcgc ctcgttaccg gtcggttcga tcccgtgaat
    10261 cacaaggcga tctggccatc gaccggaaac tactgccgcg gcggtgtcgc gatttcgcgc
    10321 atcctcggct gccgcggcgt cgccgtgctg cccgccggca tgagccaaga gcgtttcgat
    10381 tggctgcacc gctggatcgc gagacccgag gatgtcatcc ggacccccgg ctccgaaagc
    10441 aacgtgaagg agatctacga caaatgcgct gaattgtcgc gcgatccctc gaacgtcatt
    10501 ctcaaccagt tctccgagtt cggtaattat ctcgtgcact atcactgcac cggacgcgcg
    10561 ctggagtccg tgttccagga tctccgccga accgatccgg agctgcgcct ggcggccttc
    10621 gtttcggcga cgggatcggc cggcaccatc gccgccggcg attacttgaa gtccgcctat
    10681 ggcacgaaga tcgtcgcgct tgaagcgacg gagtgcccga ccatgctctt caacggctat
    10741 ggcgagcaca acatccaggg catcggcgac aagcacattc cgctgatcca caacgtgatg
    10801 aacaccgatc tcgtcgtcgg gatctcggat cgcagtaccg atgcgctcaa tctgctcttc
    10861 aacaccgaga tcggtcgggc gtatctcgag aagcggcaac atatcgacgc cgaactgctg
    10921 cgccatctcg acggcctcgg gatttccggc atcgccaacg tcctcggcgc gatcaaggtc
    10981 gccaagcatc tggagctgac cgaatccgac ctgctcgtca ccgtggcgac ggacagcgcc
    11041 gcgctctacg agagcgagcg ggagggctat atggcacgca catacccgga gggcttcgac
    11101 gaggtcaacg cagcggagat cttcgccggg catctgcagg ccctcagtga cgaccatctg
    11161 atcgagctca cccatgtcga ccggcggcgc atcttcaatc tcggctacta cacctgggtc
    11221 gagcagcagg gcgtctccgt agaggacttc gaccgtcgca gggatcccgc gttctggcgc
    11281 gagttgcagg agtcgattcc cgcatgggac cggctcgtcg aagatttcaa cgagcgaacg
    11341 ggcgtcgctg gtcggacctg agtccggcgg caagccggca cgggccgaac gcagcgacag
    11401 gagggagaag ttgacccagt cctaccttga acttcgacgc aagacagccg agcgcgacag
    11461 aagcctacgc gagaaggtca tgtcccttga agaggcagcc tcgctcgtca aggacggcga
    11521 tcaggtcggc atcggcggta gcacgttgtc gcgaacgccc atggcgatga tctgggcgct
    11581 gatccgggcg aaaaggaaga atctgtcctg ctcgcggagc atcgtctcca gcgaaggcga
    11641 cctgctcttc gcgtccgggg cgagcgacca catcctgacc agctggttca gccaaggcat
    11701 cgtctggggc gtttcgaagg tcatgcgcca ctacacggaa tcgcggaagg cgtggttcga
    11761 ggagtggagc cacatggcgg tcgggcttcg ctttcgggcc ggcgccatgg gcgttccctt
    11821 cctgccgatg cgatccatgc tcggctccga cgtggtgcgg cgcatcgagg acgtgcacga
    11881 agtcgactgc ccctttaccg gcgagaagct cgtgctggta ccggcgctga atcccgatgt
    11941 ggcgctgatc catgtccaac gctgcgacgc ctacggcaac gcccagatgg acgggctgca
    12001 gttcatggac atcgatctcg ccatggccgc gacccgggtg atcctgacga ccgagcgcat
    12061 cgtctccaac gaccagattc gccgcgcgcc ggaccagacg aaaattccgt tcttcgcggt
    12121 cgatgcggtg gtcgaggtgc ccttcggctg cgcgccgcat gagtgctacg gcgtctacga
    12181 gccgctcttc aagcacatgg actattacac ggccctggtg aacaaggatc ccgaggcggg
    12241 gacacgggca tatctcgatc gctacgtcta cggcccgaaa tcctggaccg aatatctcga
    12301 gctccttggg cttgaggagc ttctggacgc cggccgacgc ggaaggagca tctacgatga
    12361 ctagcagacg aagctacacg gccagcgagc tgctcgcggt catgagcgcc cgcctcctga
    12421 aggatggcca agtcgtcttc gccggcgtcg gcatcccgct cctcgccgcc acgctcgccc
    12481 agcggctgca cggccccggc ctcaccattc tgttcgaagg cggcaccatc ggtgccttcg
    12541 tcgagccggg caagcttccg ccatcgacca atgagcagcg ctgcacgcgg cgcgcgaaca
    12601 tggtgctcgg cagcaccgac gtgctgattc tcctgcaacg cggctatgtc gacgtcgggt
    12661 tcatgggcgg cgcgcagatc gatcagtacg gcaacctcaa cagctcgtac atcggggacg
    12721 cggacgatcc gcggattcgc cttccgggaa cgggcggcgg caatgacatc tcgagcctga
    12781 cgcagatgat cgtcgccatg aagcacgaga aacgccgctt cgtcgagaag gtcgacttca
    12841 tcaccagccc cggcttcctg cacggccgca ccagtcgcaa ggacagcggt ctgcttgcag
    12901 gcggtatgta tcgggtcgtg accgaccttg cgattctggg cttcgacgac gagacaaagc
    12961 gcatgaaggt gatcgcgctc catccgggcg tcgaagtcga ccaggttcaa gagaacacgg
    13021 gcttcgagct tgcgatcggg tccgacgtcg gcgccaccga gccgccgacg gagcaggaac
    13081 tggcggtctt gcggctgcta gatccggagc gcctctacat cgcttaagcg atcggaccgc
    13141 ctcggaaacg gaccttcacc aagttcatgg ggatgcaaat ggctggactg tcgaatacct
    13201 tcggaatcgc gatgcggaac ttcaccgcgt atccccaact ccccgacgcc cgtgggctca
    13261 tcgaatacgg cgtcaagatg gagcagatgg gattcgactc gctttgggtg tgggaccata
    13321 tcctgctcgg cgtcgatccc aactttccga tcatcgactc cctgtccctt ctgaccgccg
    13381 tcgccgcccg caccaagaag atcaggctcg gcaccggcat cctgg
//
LOCUS       DASVDU010000247        22282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1217169, whole genome shotgun sequence.
ACCESSION   DASVDU010000247 DASVDU010000000
VERSION     DASVDU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 22282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22282
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217169"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1324
                     /gene="nagA"
                     /locus_tag="VF226_16180"
     CDS             146..1324
                     /gene="nagA"
                     /locus_tag="VF226_16180"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEX7075577.1"
                     /translation="MSMQPGTTARHEAHAVLADRVFDGRHWHQNAAVLIEGARIRGVA
                     DWGEVPPDWPQHRLPVGAILAPGFIDLQVNGGGGVLLNDDPTPEAMRAIARAHRRFGT
                     TGCLPTLITDTRARTQAAIAAARSTAGRDGVLGLHLEGPFISPARPGVHRPDRIARAT
                     ADDLEWLADLGKAGASLITLAPECVPPGFVRGLTEAGVRVSVGHSEATAEDVMRAMAD
                     GLSGVTHLFNAMPPLAGRAPGIVGAALAEPRLTAGLIVDGIHVDLINVRAAFAAKGAD
                     GIALVTDAMPTVAAQTDRFQLMGRTITLKDGRLTAENGTLAGAHLDMASAVRNAVRLA
                     GVPLEDALRAASLTPARFLGLDHERGAIRAGAYADLVALTPDFDVIATWVEGNFRSEE
                     "
     gene            complement(1337..2173)
                     /gene="blaOXA"
                     /locus_tag="VF226_16185"
     CDS             complement(1337..2173)
                     /gene="blaOXA"
                     /locus_tag="VF226_16185"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HEX7075578.1"
                     /translation="MIINRTNAVLTRRTALSLSISGVAAAALARPSARAETLERRQDL
                     YPIFTEHGAVGTFVLFDASADRLVLVNPERGPQRFVPASTFKIVNSLIALETGAVKDA
                     DEVVPYGGKPQQIKDWEKDMPMREAIKVSNVPVYQELARRIGLEQYRVWLDRLRYGNR
                     EIGTVVDRFWLDGPLRISAVEQARFVALLAQRKLDASARSQAITREILRQEEKDGAVL
                     FAKTGWYFSTTETSIGWWTGWVEREGRVLAFSLNIDMPKLSDAPKRIEIGKALLAKLG
                     AY"
     gene            2619..3857
                     /locus_tag="VF226_16190"
     CDS             2619..3857
                     /locus_tag="VF226_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075579.1"
                     /translation="MTHQTRRTVLKAGATVAGAAVLGFPAIVKAQSDKIKIGHLTPLT
                     GFLGALGEYAVMGIQMARDEINKAGGVMGRELDIMSEDSVNPATAATKAQRMLERDGA
                     TVLMGEISSASALAISQVANRNKKFFMQIGARSDVLRGKNCNRYTFHVDIPNTVMVNA
                     VGKALVRDNMVKGKRFFTLTADYVFGHDLLRAAKAFFAAHEGNLIGDELVATDVTDFS
                     PYLLKIRQANPDVVCSNLAGNQVTNLIKQYAEFGLPYPIVGFNLNTADAWAAGEGNLS
                     GTWPTVWHHDLDTPGSKAFVAAFREKHGKPPENHAWIEYVSLKMMAQAMEETKSTDTE
                     KLIAYFEKETEFDILKARKGYFRSWDHQLMQEAYPFTVKPKGQAKDQWDFLQLGQAVP
                     DPDEPLEVLAPTREQNECVM"
     gene            3955..4830
                     /locus_tag="VF226_16195"
     CDS             3955..4830
                     /locus_tag="VF226_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075580.1"
                     /translation="MQLVFLLEQVVNGLVLGGYYLLIALGLSLIFSVGGVVNLAHGAF
                     YAIGAYVSVELTRFLGFGPAVVLSPISVALLGILFERFLLRKFYTADPILGLLVTFGL
                     AMIAEQAIRIIWGAAPITVSMPAELKGSVILGNFLFSRYRLMLLAVVIVVLIGIWLLL
                     HKTSFGRVVRAGIQRPDMVAALGIRLQPYMTAIVMLGVAMAALAGALFAPIANVHPAM
                     GAEILTAAFVVVVIGGLGSFWGVVLAAVLVGVVRGVTIHFLPAAGEASIYALMALVLI
                     FRPRGLLGERIEKFE"
     gene            4842..7379
                     /locus_tag="VF226_16200"
     CDS             4842..7379
                     /locus_tag="VF226_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEX7075581.1"
                     /translation="MKPLSSISKYQPLLIGIAGVLVLPFLMAALGLTINSATAVLIFA
                     IAAMGLNVLVGFTGLVSFGHAAWFGIGAYAAALAQKHLLSGQILLPILLGVLFVALLS
                     TVLGVLILRRRGVYFALLTLALSALTYAVSFRWTEVTGGEDGLGGLERGSLGPIALDD
                     PLAYYGLVGLIGLGVLFCLLRVVRSPFGHVLVAIRENQLRTTFLGYPVDAYRLGAFVL
                     SATVTGLAGALFGFLHYLVSADATSIALSGELLAMVVIGGMHSLLGPALGAIFYVLFR
                     ELFSMWTGNWLLWFGMVFVGFVIFSPSGLIGIWGELKRRWRKPPSEAAAMSTRKIAET
                     IPLPSFLHPPLRSGTALEVEGVSKHFGGIRAVVDASLEVSPSEIHALIGPNGAGKTTL
                     FNLISGLYTPDRGTVRLYGDAIEKHTANRICEMGVARSFQITSLFRGLSIYESLRLAL
                     QAGHRARFDGWRDVDSYTDLHEQTVELIRFLGLEGIEHILGGDLSYGGQRLVDLGMAL
                     GARPQVLLLDEPLAGLAAAERERVSNLIKTISAHIPVLIVEHDIDRVLGFSDRVTVMN
                     HGQVLMAGSPEEVRSDKRVQEIYTGTGTPPVIGRSAVPVSDRREVLRFERVNAFYGKS
                     HILQDASLDVREGEIVALLGRNGAGKSTLLKTLAGLVRPASGSIKLDGREIAGLPAPD
                     IARLGIGYVPQGRGLFAGMSVADNLALGRLARKSDGSDGVVWSEEQIFEFFPRLKERM
                     NVPADYLSGGEQQMAAVARALSGNVRLLLLDEPFEGLAPSVVQELFSVFDRLRAYASI
                     IIVEHNLDLVLALADRVFALERGAVFHEGPAEPLLTNLDYRKQILWL"
     gene            7486..7812
                     /locus_tag="VF226_16205"
     CDS             7486..7812
                     /locus_tag="VF226_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075582.1"
                     /translation="MRAVGTFLLILFASTTALAQDAVMKKDSIACWSHSDFMALVGGE
                     YRMNYWGSHQQKRDYMAQGRCFALRRGERVKVERLMTSPYARIARVRKQGSARSFWTD
                     QGFSGR"
     gene            7809..7934
                     /locus_tag="VF226_16210"
     CDS             7809..7934
                     /locus_tag="VF226_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075583.1"
                     /translation="MTRRSAVALNQKLRRPFEATKDYEEGIRAFFERRPAVFKGE"
     gene            complement(7988..8290)
                     /locus_tag="VF226_16215"
     CDS             complement(7988..8290)
                     /locus_tag="VF226_16215"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX7075584.1"
                     /translation="MLASHLAMQWEVARSDGPRLPAALRGGSAGSSQLQREVPGMVER
                     DFDHPHGTVATMMKDPEIIRELAQNAGLAMPRSGLATSCAGCSCPRQRLARQHLDL"
     gene            8456..10222
                     /locus_tag="VF226_16220"
     CDS             8456..10222
                     /locus_tag="VF226_16220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7075585.1"
                     /translation="MPGQFLSRYDAGVGKGLAWCAARPFRASAVIVLLCLALFVPGVF
                     SRPPVDRTEVRYAISARTMLETGDWLIPRDRKGAEVGRPVGILWLQALSTKLLGPASY
                     DAIWTYRLPSLAGAVLAVLFLFFGMRSIAGDETAILAAGLTAHSLFLALQARLALPQA
                     AALAAAVVAQTSLARLYVGQSCLSPASRKRAGLTFWAAIGAGILLNSFIIPALAVLTI
                     IGLLIQDRSLRWLAGANHALGLVLAIAIASPWPLSFWLSIADPTPDPHLSLVAWLSLV
                     VDSQHMNHDAFPGSFILMAWFGLLPCILLAYPACQLVWRNRPRPLMRFLVAWLVPYIA
                     VLELISDKPPLYMVQYALPALALAVALLITKNPVTGPPQAVWAPSWARVIWALVGIGL
                     GAAALWLETWAGDPVTLPIIAAAAVTMICFAATAVVWPRCSTLAATTFSLAGTAVAYW
                     LFLALILPPTSTKWPSNRLADLRESLRTCYPDPPIVAGYSEPSVIFLMGFDTVTTTGS
                     RAPEYLEETPRALAIIEKSHLRRFEEAVQKRSAAAPVRVACLSGRNPFTSGRNQFFAI
                     YAKPPLPSDPTCRPPPRYRCPS"
     gene            complement(10259..10927)
                     /locus_tag="VF226_16225"
     CDS             complement(10259..10927)
                     /locus_tag="VF226_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7075586.1"
                     /translation="MYRVVVPLFLALMLPGCSLGSVPLSFGFGDSGTETPAPSSSLAS
                     LTGSTPRQPASSSAKGNGQKVASLQDSAPQNSFEKPQPGALSGRDYSSTRLDVEHARK
                     LINDYRKKHGLKPLQLSPELTNAAKEHSRDLARWDRISHYGSDGSNPWDRVKRAGYEA
                     RLAAENVGTGQASFEEVLKGWKQSPGHNKNLLMADAQHMGIALVRDPKTEFKTFWTLV
                     LAAR"
     gene            11346..12491
                     /locus_tag="VF226_16230"
     CDS             11346..12491
                     /locus_tag="VF226_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075587.1"
                     /translation="MSIAVLAEPQTDAVAPAIGLMVLARAATEQGATQAEIVRDLAPY
                     VGHKLAPAAWRETVSQAIAGLAVQRLATVTRNRVELTEAGRTAIQNELGGRPLPRAWG
                     EIRDVRLIARALGMETAANARIKRLAKPDGLRAAILERAYNLKLRGAPSPSRLRQALA
                     VVALERAFGNTLKGSIGSRTGLSAKAGRLLAGQLSRRPRDFGTDNRLVAALAAEQVGA
                     FQTDADALRTAILRNFVTQSMSAPSPAPGLAATDPPEAGLATSTARQTAVVSGPRQPR
                     PAAANRPDLEGFANIVLRVASAHAEGWSGNRKAFISRVWQGIAAQHPEWGLTEIEFKA
                     MLAEAHRTGHVVLASADLKDKKSLKDLQESAVAYKNTVWHFIRVDNE"
     gene            12691..16146
                     /locus_tag="VF226_16235"
     CDS             12691..16146
                     /locus_tag="VF226_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7075588.1"
                     /translation="MQPNAQTALPVDSLSAAFEHSITWEDDIWRADPVDVEQVHLKAR
                     RKFYDLLDAVTSTRGSSTQARILLFHGQSGAGKTHLLRALRTAAHRKGKAYFGYAQMT
                     PDVASYADYYLRRLIASLEKPYDPDNGGESALARLTHRLVSSADVLSESQLKKLREAN
                     LDELGLGKLILQLADDIISSPAFADQDLDVNVVRALLYMQRSDPRIDQRVRQYLQGRQ
                     LNELSRSAVAALDPNSGDGRAFEIIEALGKLMWTVDRAALVFCIDQVEDLRFFDDAEE
                     RFQKAVRDLIQIANRVPTSIILISCLEDFYGQVRGVLAQSYIDRIEKSGPVALLENRT
                     PEEARLIIAKRLEYGASKYAGGPTFPDPSAFFGPQFFEEFGGLSTRRLLEHAQNRLRQ
                     SNAVEDPEPEEVPEKAGFISTLAAALGFGASNDEPMAEPAVDLDYRDLWQRFTQASEA
                     EIPSNDQELMDVLVAALGLARDEWSNTIQLQVNRLEVADDVPAIDLSVTHSANGLTFD
                     SRVFLCNRPTQGGGLKRQLDKVLSHMGGRASFMLRASDFPPNRKNQTAQAFRKFRDGG
                     GRSLLVPIPDWERMLMVREFHAHHRYDPGFARWFEQAKLLSGMIPIVQLLRLDLLGRP
                     AGIAPTTAASHPSSRPSAAAPAPAANGSLVERLAKLDESHQQPAPAASQPSIPPVTEK
                     PPRRIWADWIGWGSSARTEPLPANDDLLPLSVILDENGGDGKSIYAGRETNSGKPITL
                     NRDVLKRHAAVLGGSGSGKTTLALSLIEQLLLSGIPAVLLDRKGDLCSYANPDVWRSS
                     NNDFQERRSEREKLANAIDVAVYTPGRASGRPISITLLPTGISELPEHEQQLLANLSA
                     AALGDMLHLKNSATHQKQSGTLSVALKILGSRSRTEVTLNDLIYLLEDEDPELTDLTQ
                     RMDPSGKIRRDLVAQLDSLRHRNSALFEGGGENLRMESLLGLGPYARKGRTRLSIIYT
                     GFLGDNENILFWVSQFLSEALRFCQRNPNDELQAVVMFDEADLYIPANSKPATAEPLQ
                     SLLKRARSAGLGLMLATQSPGDLDYKSRDQITSWFIGRVREDTALRKLKAAFQSESGL
                     DPAAVLPGQTVGEFHLVQEGVVRSLKAQRSLISAEQVPFDRIEQLAFETKDHDVNQLP
                     LFELK"
     gene            16781..18070
                     /gene="glp"
                     /locus_tag="VF226_16240"
     CDS             16781..18070
                     /gene="glp"
                     /locus_tag="VF226_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7075589.1"
                     /translation="MPGQARDDCFRPAQARMRHGEAIALLRARIEPVAASEPVSLKDA
                     LGRIIASPLAAPRPVPAHTNSAVDGYAFAFQDYDPGSGTSLEISARAAAGHAIEAPPP
                     PGTAVRIFTGAAMPPGLDTVAMQEDCELIGAVGDSPAHVRIPGGLKQGANVRRAGEDV
                     VAGEVLFRPGHVIRPQDLAALASIGLDGVDCFARLRLAIVSSGDELIRPGAAELAPGQ
                     VYDANAPMLAGLAALAGCATEDLGIWPDSAEEVRTRLARAAESFDVILTSGGASEGEE
                     DHMGAALAALGSRHFWQLDIKPGKPMMFGQIGRSIIVGLPGNPVAVFVCFLMYVWPML
                     RRLSGAPWPEPRRLPMKAAFDFTNRRVGRREFWRGMLVETAEGLAVDKFPRDGSGLIT
                     GLRVADGLIDVPEDVGDVRRGDTVAFIPFSEFGIISR"
     gene            18404..18853
                     /locus_tag="VF226_16245"
     CDS             18404..18853
                     /locus_tag="VF226_16245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075590.1"
                     /translation="MAKLLLLYGAAGSLKRFSVRAIFAFLGTAETVRIAEACRASEWS
                     LQVHWARDLRLLEAHEQLVLGSATCWIGDSMRREPLSHDACELYAPDCPELTHRAHRF
                     FERLWQASEPVFERQGFGADEAFNPALMQPVDRATTIGGRHGPRTSA"
     gene            19041..>22282
                     /locus_tag="VF226_16250"
     CDS             19041..>22282
                     /locus_tag="VF226_16250"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX7075591.1"
                     /translation="MTRKLCLAFLALTLLLPVHAIAQGKKFSHAGIARDAERYETTIK
                     ANVTGGTLEARNLIEAGQQALAAGDPRAATRAFAQAAGAAPDGAEAWILLARALLDTR
                     PDPNRPAERYDLPANASAAAYIGYQRASVPDLQANALALLGESLKRRQMWRPAIEAFK
                     ASLALEDVPQVREVYEALRSEHGFRLVDYSVEQDLAAPRVCLQFSENLQRGQDFSQYV
                     AVDGQDAQALVPEGKQLCIEGLSHGRRYQVLVRAGLPSEVAEVLENQAELAIYVRDRS
                     PSVRFTDRSYVLPSRGQSGIPVISVNTEKVAIEVYRIGDRSLISALMSGDLQRQLSSY
                     DLDQMRETSGARVYQGELAVAHRLNEEVTTAVPVGEAVPELKPGVYAMIAMPANSRDQ
                     YGPHATQWFIVSDLGIVTFTGDDGIHAFVRSLATTQPLPNVSVRLVARNNEVLGTATS
                     DARGYVRFEAGLTRGEGGAAPALLVAEAKNGDYAFLDLTSSAFDLTDRGVKGREPPGP
                     IDAYLFTERGVYRPGEDVHLTALVRDRAGQASSVPVTLVITRPDGVEHTRIALTNDEL
                     GGRTTTLRLAPSSMTGTWRARLHADPKDDPLANVAFLVEDFVPERLDLDIDPVSKVLA
                     PEETGIVKIAGRYLYGAPAANLAVEGDIVVKPAADGLSAFPGYHFGLADEAVEPVRAP
                     IENLTKTDAEGRAELSLMLPPVPRSARPLQAELLLRLREPGGRAIERSVALPVDLRIA
                     RLGLKPLFRDLQLRENSTAEFDVILVGSDGRRASVRGAKWELVRLEQRWQWYSRDGYW
                     TYEPMTLTRKVAAGVVDIGVDEPARVSAKVDWGRYRLEVASPEPGGPVSNIVFTAGWY
                     ADEGADSPEVLELALDKPTYTAGEIAKLKIADRQGGRVLVSVLGEGLLASQEMELPAG
                     GGEVSIPVERTWGPGAYVTAMLYRPMDEKAKRMPSRAIGVKWLPIDQSARTLSVALEA
                     PAQVRSGTTLSVPVKITGLSEGEEARVTVAAVDLGILNLTRFEAPAPEGWFYGQRRLG
                     TEIRDYYGRLIDGMRAERGRLRSGGDEGGGLAMQGSPPVEEIVSLFSGIVR"
BASE COUNT         3698 a         7208 c         7228 g         4148 t
ORIGIN      
        1 gaggatgcgt cgcggcgccc tcgcttgacg tgggttgtgc gtccattgca catgcaatca
       61 cagcgagcaa gcggggcggc tgcagacgta tgcgttgtgg cgccgcacca gagcgcgcgt
      121 cgcccaaacc ggctaagaga gtttcatgtc catgcagcca ggcacaaccg cgcgacacga
      181 agcgcacgcc gtccttgcgg atcgcgtgtt cgatggccgc cattggcatc agaacgccgc
      241 ggttctgatc gagggggcgc gcattcgcgg ggttgcggat tggggcgagg ttccgcccga
      301 ctggccacag catcggctgc cggtcggagc cattcttgcg cccggcttca tcgatttgca
      361 ggtgaatggc ggcgggggcg tgctgctcaa cgacgatccg acacccgagg ccatgcgggc
      421 catcgcccgc gcgcaccggc gcttcggcac gaccggctgc cttccgacct tgatcaccga
      481 cacgcgcgcc aggacgcagg ctgccattgc tgcggcccgc agcaccgcgg gtcgcgacgg
      541 cgtgcttggc ctgcatctcg agggtccgtt catcagcccg gcgcgtcccg gcgtccatcg
      601 gccggaccgc atcgctcgtg caaccgccga cgatctcgaa tggctggccg atttgggcaa
      661 ggcgggagcc tcgctgatca cgctggcgcc ggaatgtgtg ccccccggct tcgtgcgcgg
      721 cctcacggag gccggcgtgc gcgtgtcggt cgggcacagc gaggccacgg ccgaggacgt
      781 gatgcgcgcc atggcggacg gcctcagtgg cgtgacacat ctcttcaacg ccatgccgcc
      841 gctggccggt cgcgcgccgg gcattgtcgg tgcggcattg gccgagccgc ggctcacggc
      901 cggcctgatc gtcgacggta tccacgtgga tctgataaat gtgcgcgccg cttttgccgc
      961 caaaggagcc gacggcattg cgctcgtcac ggacgcgatg ccgacggtcg cggcgcagac
     1021 ggatcgcttt caactcatgg gacggacgat cacgctcaag gatggtcgac tgaccgctga
     1081 gaacggcacg ctggcgggcg cgcatctcga catggcttcg gcggttcgta atgccgtccg
     1141 gctggcaggc gtgccgttgg aagatgcgct gcgcgccgcg tcgctgacac ccgcgcgctt
     1201 ccttgggctc gatcacgagc gcggcgccat ccgtgccggc gcttacgccg atctcgtggc
     1261 cctcacgccc gatttcgatg tcatcgccac gtgggtggag gggaattttc ggtcggagga
     1321 atagcttccg atgccctcaa taggcgccaa gctttgcaag cagcgccttg ccgatttcga
     1381 tccgtttcgg ggcgtcggac agtttcggca tgtcgatgtt gagcgagaag gcgaggacac
     1441 gtccttcccg ctcgacccag cctgtccacc agccgatcga agtctccgtc gtcgagaagt
     1501 accagcccgt tttggcgaag aggacggcgc catccttttc ctcctgccgg agtatctccc
     1561 gggttatggc ttgcgagcgc gcgctggcgt cgagctttcg ctgggcaagc agcgcaacga
     1621 accgggcctg ctcgacggcg ctgatccgca gtggtccatc gagccagaac ctgtcgacca
     1681 ccgtaccgat ctcgcgattt ccgtaccgca ggcggtcgag ccacacccgg tactgctcga
     1741 gaccgatacg gcgcgccagc tcctgataaa ccggcacgtt cgagaccttg atggcctcgc
     1801 gcatcggcat gtccttttcc caatccttga tctgctgcgg cttgccgccg taggggacga
     1861 cctcgtcggc gtccttcacg gcgcccgttt cgagagcgat gaggctgttc acaatcttga
     1921 acgtcgaggc ggggacgaac cgctgcgggc cgcgctcggg attgacgagc acgaggcggt
     1981 ccgcactcgc gtcgaagagc acgaatgtgc ccacggcacc atgctcagtg aagatcggat
     2041 agaggtcctg acgccgctcc agcgtctcgg ccctggctga gggacgcgcg agtgccgccg
     2101 ctgccacgcc actgatcgaa aggctgagtg cggttcttct ggtgaggacg gcgttggttc
     2161 gattgatgat catgggactc cgggctcgca tctgaagttg cgcgcgggca tacgtgcgag
     2221 cttcgtcaac acttcggcag accgggattt cccacagcag cctactcgaa cgaggcggtg
     2281 atcgaaacct cttcccgacg ccggcgagct agtcgcgcgg tccattgagg cagatctcgc
     2341 cgagcggggg ccggggctca cgaagcaggc cagcgaagcc gctttagcca gtgttatccg
     2401 cgtcccgatt ctcccttggg tagaggcgat ccgaggcctt ctgcggaaac ttggttttca
     2461 gcatgtggga cgtcaattta taatggcaaa caagctgacc gcctgtaatg ttggagtgct
     2521 gggccgcgcc ggtgcagcaa accaagttgc cgggtccggt ctgcagtccg gccccggcgg
     2581 ataatcaaga gcaacggagg caaggaggaa aacgcttgat gacacatcag acccgccgaa
     2641 ccgtattgaa ggccggcgcc accgtcgctg gcgctgccgt gctcggcttt cccgccattg
     2701 tgaaggctca gtcggacaag atcaaaatcg gccacctcac gccattgacc ggctttctcg
     2761 gcgcactcgg cgagtatgcc gtgatgggca ttcagatggc ccgggatgag atcaacaagg
     2821 ctggcggcgt catgggccgc gagctcgaca tcatgtcgga ggattccgtc aacccggcga
     2881 cggctgcgac caaggcgcag cgtatgctgg agcgcgacgg cgcaaccgtg ctgatgggcg
     2941 agatctcatc ggcctcggcg ctcgccattt cgcaggtcgc caatcgcaat aagaaattct
     3001 tcatgcagat cggcgcccgc tccgacgtgc tgcgcggcaa gaactgcaat cgctacacct
     3061 tccacgtcga tattccgaac acggtcatgg tcaacgcggt cggcaaggcg ctcgttcgcg
     3121 acaacatggt gaagggcaag aggttcttca ccttgaccgc tgactatgtg tttggccacg
     3181 acctgctgcg cgccgccaaa gccttcttcg cggctcatga aggcaatctg atcggcgatg
     3241 agctggtcgc cacggacgtg accgatttca gcccgtatct attgaagatc cgccaggcca
     3301 atcccgacgt ggtctgctcg aacctggccg ggaatcaggt gacgaacctc atcaagcaat
     3361 acgctgagtt cggtctgcct tatcccattg tcggattcaa tctcaacacg gcggatgcct
     3421 gggccgcagg tgaaggcaac ctgagcggca cctggccgac ggtctggcac cacgacctcg
     3481 acactcccgg ctcgaaggcg ttcgttgccg ccttccgtga gaagcacggg aagccgccgg
     3541 aaaatcatgc ctggatcgag tacgtctcgc tcaagatgat ggcgcaggca atggaggaga
     3601 cgaagtcgac cgacacggag aagctgatcg cgtatttcga gaaggagacg gagttcgaca
     3661 tcctgaaggc ccgcaagggc tacttccgct cctgggacca tcagctcatg caggaggcct
     3721 atcccttcac ggtgaagccc aaggggcagg cgaaggacca atgggacttt ctgcagctcg
     3781 gccaggcggt gcccgatccg gatgagccgc tggaagtgct cgccccgacc cgcgagcaga
     3841 acgagtgcgt catgtgacgt gatccggctc gggcccactc gcccgagcct tcattaaagg
     3901 gcgccctgcg gtctcggcat cgtggggacg ccctccgata ttcacggatc gatgatgcag
     3961 ctcgtctttt tgctcgagca ggtcgtcaac ggcctcgtcc tcggcggcta ttacctgctg
     4021 atcgcgctcg gcctgtctct gattttcagc gtcggtggcg tggtgaacct cgcgcatggc
     4081 gcgttctacg ccatcggcgc ctatgtgtcg gtcgagctca cgcggtttct cggttttggc
     4141 ccagcggtcg tgctctcgcc gatatcggtg gcgctgctcg gcatcctttt cgagcgtttc
     4201 ttgctgcgca aattctacac cgccgacccg atcctcgggc ttctggtgac gttcgggctg
     4261 gcgatgatcg ccgagcaagc gatccgcatc atctggggcg cggcgccgat taccgtttcg
     4321 atgcccgcgg agctcaaagg aagtgtgatc ctggggaatt ttctgttttc ccgctaccgc
     4381 cttatgttgc tcgcggtggt gatcgtcgtc ctgatcggta tatggctgct tctgcacaaa
     4441 acttcattcg gccgcgtcgt gcgcgccggc attcagcgcc cggacatggt ggccgcgctc
     4501 gggatccggc tgcagcccta catgacggcc atcgtcatgc tgggtgtcgc gatggcggcg
     4561 ttggccggcg ccctttttgc gccgatcgcg aatgtgcacc cggccatggg cgcggagatc
     4621 ctgactgcgg cgttcgtcgt cgtagtcatc ggcggcctcg gcagcttctg gggcgtcgtg
     4681 ctggcggccg tgctcgtcgg cgtcgtccgt ggtgtcacga tccatttcct gcccgctgca
     4741 ggcgaggctt cgatctacgc gctcatggcg ctcgtcctga ttttccgacc gcgcggcctg
     4801 ctcggtgaac gcatcgagaa gttcgagtga ggagcggtcg catgaaacct ctgagctcga
     4861 tctcgaagta tcagccgctg ctgatcggca ttgcaggcgt tctcgtcctg ccgttcctga
     4921 tggcggcgct cggccttacg atcaacagtg ccacggcggt gctcatcttt gccatcgccg
     4981 cgatggggct gaacgtgctc gtcggcttca cggggctcgt ttctttcgga catgccgcct
     5041 ggttcggcat cggagcgtat gcggcggcgc ttgcccagaa gcacttgctg tccggacaaa
     5101 ttctgctccc cattctgctc ggagtgcttt tcgtcgcgct gctttcgacc gtgctcgggg
     5161 tcctcattct gcggcggcgc ggcgtgtact ttgcactcct gacgctggca ctttcggcgc
     5221 tgacctatgc cgtcagcttc cgctggacgg aggtgacggg cggggaggac ggcctcggcg
     5281 ggctcgagcg cggcagcctc ggccccatcg cgctcgacga tccgctcgcc tattacggtc
     5341 tcgtcggcct gatcggactc ggcgtgctgt tctgcctcct gcgcgtcgtg cgctcgccct
     5401 ttgggcatgt gctggtggca atccgtgaga accagctgcg cacgacgttt ctcggctatc
     5461 ccgttgacgc gtatcgcctc ggcgcgttcg tgctctccgc gacggtaacc ggtcttgccg
     5521 gcgcgctgtt cggtttcctc cactacctcg tctctgcgga tgccacatcg atcgcgctat
     5581 ccggcgagtt gctggcgatg gtcgttatcg gcggcatgca cagcctgctt ggcccggcgc
     5641 tcggcgcgat tttctatgtc ctgttccgcg agctgttttc catgtggacg ggaaactggc
     5701 tgctctggtt cggcatggtc ttcgtcggct ttgtgatctt ttcgccgagc ggcctcatcg
     5761 gcatctgggg cgaactgaaa cggcgctggc gcaagcctcc ttccgaagcc gcagccatga
     5821 gcacgcggaa gatcgcggaa acaattccgt tgccgtcatt tctgcacccg cctctgcgct
     5881 cgggcaccgc gctcgaagtc gagggcgtct caaagcactt cggcggtatc cgtgctgtcg
     5941 tcgacgccag cctggaagtc agccccagcg agattcacgc gctgatcggc ccgaacggcg
     6001 ctggcaagac gaccctgttc aacctcatct ccggtctcta cacgccggat cgcggcaccg
     6061 tgcggcttta tggggatgca atcgagaagc atacggcgaa ccgcatctgc gagatggggg
     6121 tcgcccgatc gttccagatc acgagccttt ttcgcgggct ctccatctac gaaagcctcc
     6181 gcctggcttt acaggccggc cacagagccc ggttcgacgg ctggcgcgat gttgacagtt
     6241 atacggatct ccacgagcaa acggtggagc tgatccgttt cctcgggctc gaagggatcg
     6301 agcacatcct gggtggcgac ctttcctatg gtggtcagcg gctggtcgat ctcggaatgg
     6361 cgcttggcgc gcggccgcag gtgttgctgc tcgatgaacc gctggccggt ctcgcggctg
     6421 ccgagcgcga acgcgtttcc aacctcatca agaccatttc ggcccacatc cctgttctca
     6481 tcgtcgagca cgacatcgac cgcgtcctcg gtttttccga ccgcgtcact gtcatgaacc
     6541 acgggcaggt gctgatggct ggctcaccag aggaggtgcg ttcggacaag cgtgtgcagg
     6601 agatctatac gggcaccggc acgccgcccg tgatcggccg cagtgccgtt ccggtgtcgg
     6661 atcgccgcga ggtgctgcgg ttcgagcggg tcaacgcctt ctacggcaag agccacatcc
     6721 ttcaggatgc gagcctggat gtccgggagg gcgagatcgt cgcgctgctc gggcgtaacg
     6781 gcgccggcaa gtcgacactg ctgaagacgc tggctggtct cgtccgcccg gcttccggca
     6841 gcatcaaact cgatggccgg gagatcgccg ggcttcccgc cccggatatc gcccgcctcg
     6901 gcatcggcta cgtgccgcag ggccgcgggc tgttcgccgg catgtccgtt gccgataacc
     6961 ttgcgctcgg ccgcctcgcc cgcaagtcgg acggcagcga tggcgtggtg tggagcgaag
     7021 agcagatctt cgagttcttc ccccgcctca aggaacgcat gaacgtgccc gcggactacc
     7081 tgtccggcgg tgagcaacag atggcggcgg tcgcgcgcgc cctttcaggt aacgtgcgcc
     7141 ttctgctcct cgacgaaccg ttcgagggcc tcgccccatc ggtggtccag gagttgttca
     7201 gcgtcttcga ccgcctgcgc gcgtatgcgt cgatcatcat cgtcgagcat aatctcgacc
     7261 tcgtcctcgc gctggcggac cgtgttttcg cgctcgagcg cggtgcggtg ttccacgaag
     7321 gcccggccga gccgcttctg acgaacctcg attaccgcaa gcaaatcctt tggctttgag
     7381 gctttggggc ctgggatccc gccggcgagc gatggatcga ttggcgcaac agagggtttt
     7441 ccagcctgaa catgcggctg agccggaaca gatcaggttg ggaatatgcg cgccgttggc
     7501 acatttctgc tcattctttt tgcctccacc acggccctgg cgcaggacgc cgtcatgaag
     7561 aaggactcga tcgcttgctg gagccacagc gatttcatgg cactggtcgg cggtgagtac
     7621 cggatgaact attggggcag ccaccagcag aagcgcgatt acatggccca aggccgctgc
     7681 ttcgctttga gacgaggcga gcgcgttaag gtcgagaggc tgatgacaag cccttatgcc
     7741 cgtattgcca gagtgcgcaa gcaaggcagc gcccgaagct tctggaccga ccaggggttc
     7801 agcgggcggt gactcgccgc tctgcggtgg cgctgaacca gaagctccgc cgtccgttcg
     7861 aggctaccaa ggactacgag gagggcatcc gcgccttctt cgagaggcgc ccggcggtat
     7921 tcaagggcga gtgaagcagc acgcccccga agatcttccg ccttattcgc gccgcccctc
     7981 aattccctca aagatcgaga tgctgtcgcg ccagccgttg ccgcgggcaa ctgcagccgg
     8041 cacagctcgt tgcgagtccg gaccgcggca tggcaagccc ggcattctgg gcgagctcac
     8101 gaatgatctc ggggtccttc atcatggttg cgaccgtgcc gtgcgggtga tcaaagtcgc
     8161 gctcaaccat gcccggcacc tcgcgctgca attggctgga acccgcggag ccgccgcgca
     8221 gcgccgccgg cagccgcggt ccgtctgacc gcgcgacctc ccattgcatg gctagatggc
     8281 tagcgagcat cttcgcgaag cgctcccgag cgtgtaagct cgtaagcgca gctctcacct
     8341 atgccaaagc ttccaaaagc ctgaccaatc ttactcttac gatgagtgtc gccgacgtgg
     8401 actgcgggga tgccagcgga ccggcccttc ggggccgggg atgtgagcgc ggctgatgcc
     8461 aggccagttc ctctcgagat atgacgccgg ggtaggcaag ggcctcgcct ggtgcgccgc
     8521 acgtccgttc cgcgcctcgg cggtgatcgt cctgctttgt ctggcgttgt tcgtaccggg
     8581 agtgttctct cgtccacctg tggatcgcac ggaagtccga tatgcgatct ctgccaggac
     8641 gatgttggag acgggcgact ggctcatccc gcgcgaccgg aagggggcgg aggtcggcag
     8701 gccggtcggc atcctgtggc tgcaggcgct ctcaaccaag ctgctcggcc cggcatcgta
     8761 tgacgcgatc tggacgtatc gcctcccctc cctggcagga gcggttctgg ccgttctgtt
     8821 tctgttcttt ggaatgcgct ccattgccgg cgatgagacg gcaatcttgg ccgccggtct
     8881 gacggcccac tctctgttcc tggctttgca ggctcggctg gccttgccgc aggcagcggc
     8941 actggcagct gcggtcgtcg cacagacaag cctcgcccgt ctctacgtgg ggcagtcctg
     9001 tctctcacct gcgtctcgaa agcgtgccgg gctgacgttc tgggcggcca tcggcgccgg
     9061 aatccttctc aactcgttca tcatcccggc cctcgccgtc ctcacgatca tcggcttgct
     9121 gatccaggac cgcagcctca ggtggctcgc cggggcgaac cacgcgctcg gtctcgtgtt
     9181 ggcgatcgcg atcgcatcgc catggccact ttcgttctgg ctttcgatcg ccgacccgac
     9241 gcccgacccg catttgtcgc tcgtcgcatg gctgtccctc gtggtggact cccagcacat
     9301 gaaccacgac gcctttccag gcagcttcat tctcatggcc tggttcggcc tgttgccgtg
     9361 cattctgctc gcctaccccg cctgccagct ggtctggcgg aacagaccgc ggcccctgat
     9421 gcgatttctg gtggcgtggc tcgtacctta catcgcggtg ctcgagctga tctcggacaa
     9481 gccgccgctc tacatggtgc aatacgcatt gccggcgctg gccttggcag tggcacttct
     9541 aataacgaag aatcccgtca cgggtccgcc tcaggctgtc tgggcgccct cgtgggcgcg
     9601 cgtgatctgg gccctcgtgg gcatcggtct gggtgcagcg gcgctctggt tggagacgtg
     9661 ggccggagac cccgtgaccc tgcccatcat cgcagcggcg gcggtgacga tgatctgctt
     9721 tgctgccacg gccgtcgtct ggccgcgttg cagcaccctc gcagcaacca ctttctcatt
     9781 ggccgggacc gccgttgcct attggttgtt cctcgctctc attttgccgc cgacgtcgac
     9841 gaagtggccg agcaatcggc tggcagacct gagagaaagc ctgcggacct gttatccgga
     9901 ccccccgatc gtcgccggat attcggagcc gagcgtcatt ttcctcatgg gcttcgacac
     9961 cgtcactacg acgggatcac gcgcgccaga gtacctggaa gagacgccgc gcgccttggc
    10021 gatcatcgag aagtctcatc tgcggcggtt cgaagaggcc gtgcaaaagc gcagtgccgc
    10081 cgcgccggtc cgcgtggcct gcctgtccgg ccgtaatccc tttacgtcgg gaagaaatca
    10141 gttcttcgcg atctacgcga agccaccact gccatctgat ccgacctgcc ggccgccgcc
    10201 gcgctatcgc tgcccgagct agcccgcgtc ggcttggcta gccttgggcc ggagcccgct
    10261 agcgtgcagc gagaaccagc gtccagaacg tcttgaactc ggtcttcgga tccctcacca
    10321 gggcgatgcc catgtgctgg gcgtccgcca tcagcaggtt cttgttgtgg cccgggctct
    10381 gcttccaacc cttcaacact tcctcgaagc tcgcctgtcc cgtgccgacg ttctccgccg
    10441 cgagccgcgc ttcatagccg gcgcgcttca cgcggtccca agggttggag ccgtccgagc
    10501 cgtaatggga gatgcggtcc caacgggcga ggtcgcgcga gtgctccttc gccgcgttgg
    10561 tcagctccgg gctaagctgc aacggcttga ggccatgctt cttgcgatag tcgttgatca
    10621 gcttgcgagc gtgctcgaca tcgagccgcg tgctcgagta gtcacggccg gaaagcgcgc
    10681 cgggctgggg cttttcgaat gagttctgcg gtgcactgtc ctggaggctg gcaaccttct
    10741 gtccgttccc tttcgccgaa gacgaagcag gttggcgcgg cgtgctgcct gtcagcgacg
    10801 cgagcgacga gctcggcgca ggagtctctg taccggagtc gccgaatccg aaggaaagtg
    10861 gtaccgagcc gaggctgcac cctggcagca tcaatgcgag gaagagaggg accacgacgc
    10921 gatacatccg gatactcccg ctcaaaagcg gaccagcttg caggatcgcc tagggcggtt
    10981 aagacagcgt taacgctaac ggctggtaag catgctcccg cggcggcccg gcgtggcgcc
    11041 aggtggtcca cggtggtcga tactcgtgtc cgtcagcttt cgcagttgac ttcaagacct
    11101 tgacggcagt ggggcaatcg cgcggcttgt tgatcgcggt tcgtgcgctg aggagctgcc
    11161 gcgaggaccg gatggagaag aaagcgaccg attagcactt ggcaccgcgc cgccgatccg
    11221 gagaattgag aaagtctgaa gtgattcgtg cgtctggcca acgagtttga cgctggcgct
    11281 gtcgcgcgcc gaaatcgaat tggcgggaca cggaagtctt agcccggccg ggaggtcgag
    11341 agaagatgag catagccgtc ttggcggaac cacaaaccga tgccgtcgct cctgcgatcg
    11401 gcctgatggt gctggcgcgg gcggcgacgg agcagggtgc gacgcaggcc gagatcgtac
    11461 gcgatctggc gccctacgtc ggccataagc tcgcacccgc tgcctggcgt gaaaccgttt
    11521 cccaggcgat cgccggcctc gcggtgcaac gcctcgcgac cgtgacacgg aaccgggtcg
    11581 agctgacgga agccggacgc acggccatac agaatgagct cggtggcagg ccgctgccgc
    11641 gtgcgtgggg agagatacgg gacgttcggc ttatcgcccg ggcgctcgga atggagacgg
    11701 cagcgaatgc ccgcatcaag cgcctggcca agcccgacgg cctgcgcgct gccattctgg
    11761 aacgtgctta caacttgaag ctccgtggtg cgcccagtcc gtcgcggctg cgccaggcct
    11821 tggcggtggt ggcgctcgag cgggccttcg gcaatacgct caagggcagc atcgggtcgc
    11881 gaacgggact ttcggccaag gcggggcggc tgctagcagg gcagctttca cgccggccgc
    11941 gcgatttcgg cacggataac cgcctcgtcg cggcgctcgc ggccgagcag gtgggtgctt
    12001 tccaaacgga tgccgacgcg ctgcggacag ccattctgcg caatttcgta acccagtcga
    12061 tgtcggcccc gtcgccagcg ccaggtcttg ctgcaacgga tccaccggaa gcagggctcg
    12121 ctacatcgac ggcgcgccag acggcagtgg tgtcgggccc gcgccagccg cgtccggccg
    12181 cagcaaaccg gcctgatctc gaaggcttcg cgaacatcgt gctgcgggtg gcgagcgcgc
    12241 acgcagaggg atggtcgggc aatcgcaagg cgttcatctc ccgcgtgtgg caaggcatcg
    12301 ccgctcagca cccggagtgg ggcctcaccg aaatcgagtt caaggccatg ctggcggagg
    12361 cgcaccggac cggtcacgtg gtcctggcaa gtgccgacct gaaggacaag aagagcctga
    12421 aggacctgca ggagtctgcc gtcgcctaca agaataccgt ctggcacttc atcagggtcg
    12481 ataacgaatg atgcggacag ggcagaccag atggcaacca tcccggtgct cctcaccggg
    12541 atcactcgtc cgctcgcacg ggcgcctgcg gtccgctgga tcccgggaac aaggcccggg
    12601 atgaccactg aattggtgca gcgagtcaac ttcgaataac cgggcacaac gacgagttcg
    12661 gatctgtccg cgtttgagga attggggatc atgcagccga atgcgcaaac cgcgcttcct
    12721 gtcgacagcc tttccgccgc gttcgagcac tcgatcacgt gggaggacga catctggcgt
    12781 gccgacccgg tcgacgtcga gcaggtgcac ctcaaggcgc gccgcaagtt ttacgacctg
    12841 ctggacgcgg tcacgtcgac gcggggatcg agcacgcagg cgcgcattct gctgttccat
    12901 ggccaatcgg gcgcgggcaa gacccacctg ctccgggcgc tgaggaccgc cgcgcatcgg
    12961 aagggcaagg cttatttcgg ctatgcccag atgacgcccg acgtggcgag ctacgccgat
    13021 tattacctcc gtcgtttgat cgcctccctc gagaagccct acgatccgga caacggcggc
    13081 gagagcgccc tggcgcggct cacgcaccgt ctcgtctcct ccgcggacgt gctgtcggaa
    13141 agccagctca agaagctgcg cgaggcgaac ctggacgagc tcggtctggg caagctgatc
    13201 ctgcagctcg cggacgacat catatcaagt cctgccttcg ccgatcagga cctcgacgta
    13261 aacgtggtgc gggcgctgct ctatatgcag cgcagcgatc cccgcatcga tcagcgggtg
    13321 cgtcaatacc tgcagggccg ccagctcaac gagttgtctc gctctgcggt ggctgcgctc
    13381 gatccgaaca gcggtgacgg ccgcgccttc gagatcatcg aggccctcgg caagctgatg
    13441 tggaccgtcg atcgggcagc gctcgtgttc tgcatcgatc aggtcgagga cttgcgcttc
    13501 ttcgacgacg cggaggagcg cttccagaag gcggtgcggg acctcatcca gatcgccaac
    13561 cgcgtgccga catcgatcat tctcatctcg tgcctggagg acttttacgg ccaggtccgc
    13621 ggcgtgcttg cgcagtccta catcgatcgc atcgagaagt ccggtccggt cgccctgctc
    13681 gagaaccgca cgccggagga ggcgcggctc atcatcgcca agcgcctcga gtacggggca
    13741 tcgaaatatg caggggggcc gaccttccct gatccgtccg cctttttcgg cccgcagttc
    13801 ttcgaggagt tcggtggcct ctcgacccgg cgcttgctgg agcacgccca gaaccgcctg
    13861 cggcagtcga acgccgtgga ggatcccgag ccggaggagg tgccggagaa ggctggcttc
    13921 atctcgacgc tggccgcggc gctcggtttc ggggccagca acgacgagcc gatggcggag
    13981 cctgcggtgg atctcgacta tcgcgacctg tggcagcggt tcacgcaggc ctccgaggcc
    14041 gagattccct cgaacgacca ggagctgatg gacgtcctcg tcgccgcgct ggggctcgcg
    14101 cgcgatgagt ggagcaacac gatccagctc caggtcaacc gcctcgaggt ggcagacgac
    14161 gtcccggcga tcgacctgtc ggtgacccac agcgcgaacg ggctgacatt tgattcgcgc
    14221 gtgttcctct gcaaccggcc gacccagggt ggcggcctca agcgacagct cgacaaggtg
    14281 ctgagccaca tggggggacg cgcctcgttc atgctgcgcg ccagcgattt cccaccgaac
    14341 aggaagaacc agacggcgca ggcgttccga aaattccgcg atggcggcgg ccgctcattg
    14401 ctcgtgccga tcccggattg ggagcgcatg ctcatggtgc gcgagttcca tgcgcatcac
    14461 cgttatgacc cgggtttcgc gcgctggttc gagcaggcga agctgctgtc cgggatgatc
    14521 ccgatcgtgc agcttctgcg cctcgatctc ctgggacggc cggcgggtat cgctccgaca
    14581 actgctgcat cgcatccgtc gagccggccg agtgctgccg cgccggcgcc tgccgcaaac
    14641 ggatcgctgg tcgagcggct ggccaagctg gacgaaagcc atcagcagcc cgctcctgcc
    14701 gcgtcccagc cctccattcc gcccgttacg gaaaagccgc cgcgtcggat ttgggccgac
    14761 tggatcggct ggggctcttc tgccaggacc gagccgcttc cggccaatga tgacctgctg
    14821 ccgctgtcgg tcatccttga tgaaaatggc ggcgacggaa aaagcatcta tgccgggcgg
    14881 gaaaccaaca gcgggaagcc gatcacgctc aatcgcgacg tgctgaaacg gcacgcggcc
    14941 gtgctcggcg gctccggctc gggtaagacg accttggcgc tgagtctcat cgagcaactg
    15001 ctgctgagcg gcattcccgc ggttctcctc gaccgcaaag gcgacctgtg cagctacgcc
    15061 aatccggacg tctggcgctc gagcaacaac gattttcagg aacgccgcag cgagcgcgag
    15121 aagctcgcca acgccatcga cgtcgcggtc tacacgccgg ggcgcgcctc cggccggccg
    15181 atctcgatca cgctgctgcc caccggcatc agcgagctgc ccgagcacga acagcagctt
    15241 ctggccaatc tctcggcagc cgcgctcggt gacatgctgc atctcaagaa ttctgccacc
    15301 caccagaagc agtccggcac gctatcggtg gcgctcaaga tcctgggcag ccggtcgcgc
    15361 accgaggtca cgctcaatga cctcatctat ctcctcgagg acgaagatcc ggagctgacg
    15421 gatctgaccc agcgcatgga cccctcgggc aagatccggc gcgacctggt ggcccagctc
    15481 gacagccttc gccacaggaa ctcggccctg ttcgaagggg gcggcgagaa cctccgcatg
    15541 gagtcgctgc ttgggctcgg gccctatgcc cgcaagggcc gcacgcggct gtcgatcatc
    15601 tacaccgggt tcctgggcga caacgagaac atcctgttct gggtgtcgca gttcctgtcg
    15661 gaagccctgc gcttctgcca gcgcaacccg aatgacgagc tgcaggccgt cgtcatgttc
    15721 gacgaggccg acctctacat cccggccaat tcgaagccgg ccacggccga gccgctgcag
    15781 agcctgctga agcgcgcgcg gtccgccggc ctaggcctga tgctggccac ccagtcgccg
    15841 ggtgatctcg actacaagag ccgagaccag atcacgtcct ggttcattgg ccgcgtgcgc
    15901 gaagatacgg cactgcgcaa gctcaaagcc gcctttcaga gtgagagcgg gctcgacccg
    15961 gcggccgtgc tgcctgggca gaccgtcggc gagttccacc tcgtgcagga gggcgtggtg
    16021 cgatcgctca aggcccagcg ctcgctcatc agcgccgagc aggttccgtt cgaccgcatc
    16081 gagcagctcg ccttcgagac caaggatcac gacgtcaatc agctgccgtt gttcgagctg
    16141 aagtagccgc ccgagagccg tggcgatcga cccggcttat gccatgttgc gcgcttgcaa
    16201 caaccttcgg ccactttgat ccgggggcga cttctcaaca ggcaggatgc tgccggtgtc
    16261 ttgtgatgaa cagcttgtac acgttctgtt aagcccagcg gttcacaatg gaacttgggc
    16321 attctcccgt ggagattgca ggtgacccta gagcagctca cttcaatgca ttgcagctgc
    16381 agcagtcctg caatttcgag gtgcattgtt tgggaaccgg gtgtattctg agtacgtgca
    16441 gcacgcgagg agggggcggg tgcggcacgc ggaaatggtg agctcggcgc aacaggggga
    16501 tttgccatcg agcggcgttg tttgttgcgg ggttgtgtca tgacggcgaa gccgttgctt
    16561 gtaatgttgc ttgccacgct tcttgggcct gccatcggcg tcggtctgtt cattctcatg
    16621 cagacgctgt agttccttac tttttcctgc ggatcgctca atccacgagg cgagctggcc
    16681 gaattgtcgc ccgccgcttc tcgtttcctc gacaccgtcc taatgtgatg cccagcggat
    16741 caagggaggc tcgctcctcc tgagtgcggg actttctcac atgcccggcc aggcgcgcga
    16801 cgactgcttc aggcctgccc aggcgcgtat gcgtcatggg gaggccatcg cgctactgcg
    16861 cgcgcggatc gaacctgtcg cagcctccga accggtttcg ctgaaagacg cactcgggcg
    16921 catcatcgca agcccgctgg cggcgccgcg gcccgtcccg gcgcatacga actccgccgt
    16981 cgacgggtat gccttcgcat tccaggacta tgaccccggc tctggcacat ctctggaaat
    17041 cagcgcgcgg gcggctgctg ggcatgccat tgaagcaccg ccgcctccgg gtacggccgt
    17101 gcgcatcttc accggagccg cgatgccgcc gggtctcgac accgtcgcga tgcaggagga
    17161 ctgcgagctg atcggagccg tcggcgactc accggcacac gttcgcatcc cgggcggcct
    17221 gaagcagggc gctaacgtac gccgcgccgg ggaggatgtc gtcgcaggcg aggtgctgtt
    17281 ccgccccggc cacgtgatac ggccacaaga cttggctgcc ctcgcctcga tcggtctgga
    17341 cggtgtcgac tgcttcgcgc ggttgcgcct tgccatcgtc tcctccggcg atgagctgat
    17401 ccggccagga gcagcggaat tggctcccgg ccaggtctac gacgccaacg cgccgatgct
    17461 ggccggtctt gcagcactcg cgggatgcgc gacggaggat ctcggcatat ggccggacag
    17521 cgcagaggag gtccgcaccc gtctcgcccg ggcagccgag tcgttcgatg tcatcctcac
    17581 gtcaggcggg gcgagcgagg gggaagagga tcacatgggc gccgcgctcg cagccctcgg
    17641 ctcacgccat ttctggcaac tcgacatcaa gccgggcaag ccgatgatgt tcggccagat
    17701 cggccgcagc atcatcgttg gtctgcccgg caatccggtc gccgtgttcg tctgtttcct
    17761 gatgtacgtt tggccgatgc tccgccgcct gtcgggggcg ccctggccgg agccgcggcg
    17821 tctgccgatg aaagccgcgt tcgactttac caatcgcagg gtcggccggc gcgaattctg
    17881 gcgcggcatg ctggtcgaga ccgccgaggg attggccgtc gacaagttcc cacgcgacgg
    17941 ctccggtctc atcacagggc tcagggtggc tgatggcctg atcgacgttc cggaggatgt
    18001 cggggatgtg aggcgcggcg ataccgtcgc cttcattccg ttctctgagt tcggtatcat
    18061 aagccgctga tggcagtggc atgacccccc ggtggccgta atgtgaagag cggccctgat
    18121 gatccacaga tgaaccgacc gctcatcaat cattaaccgc gttccctctt taattgtgtc
    18181 ccgaggcccc acaagcggcg cgtgcctgat gctccgaaag cgccagagag gatcgcatat
    18241 gtctcgacta gctgttcctc aatcccacct cgtcaaacgc gaagagaagg aagacaagct
    18301 cgaaaggctg ctccgtcgcc acctcagagc cctggctgaa agcacaccct gcgatgcagc
    18361 cgaaacgaat tgcctgctaa tcgctcgctc cgctgacagt ccggtggcca agctcctctt
    18421 gttgtacggt gccgccggca gcctgaagcg cttttccgtg cgcgcgatct tcgccttcct
    18481 cgggaccgcc gagacggtgc ggattgcgga agcctgccgt gcctccgagt ggtcgctgca
    18541 ggtgcactgg gcgcgggatc tgcgcctgct cgaagcgcat gagcaactcg tcctcggttc
    18601 cgcgacctgc tggatcggcg acagcatgcg ccgggagccg ctgagccacg acgcctgcga
    18661 gctctatgcg ccggactgcc cggaactgac gcaccgggcg caccggtttt tcgagcgtct
    18721 gtggcaggcg tccgagccgg ttttcgaacg ccagggattt ggcgccgacg aggcattcaa
    18781 tcccgcgctg atgcaacccg tcgatcgggc gaccaccatc ggcggcaggc acggccctcg
    18841 aacctccgcg tgaacctgcc gtcaggcagg agcggaaacg accggcaagg agaggcaagt
    18901 ccgccgaact cgggatacgt tccggcgcat tgcctctgtt cgatcactgc gacttgggcg
    18961 agtgttgggg tgttcgcctg cccgtcggag cctcggcgga gtacgatcag ggcaggtccc
    19021 ccgcgcgaag ggatgttgcc atgactcgca agctgtgcct cgccttcctc gccctcaccc
    19081 tcctcttgcc tgtccatgcg atcgcccagg ggaaaaagtt ctcgcacgcc gggattgccc
    19141 gggatgccga gcggtacgaa acgactatca aggccaatgt caccggcggt acgctggagg
    19201 cacgcaacct gatcgaggcc ggacagcagg ccctcgccgc aggcgatccg agggccgcca
    19261 cgcgcgcctt cgcgcaagcg gctggcgcag cgcccgacgg ggcagaggcc tggatcctgc
    19321 tcgcccgtgc cctgctcgac acgaggcctg atccgaaccg tccggccgaa cggtacgatc
    19381 tgcccgccaa tgcaagcgct gccgcctaca tcggctatca gcgggcatcg gtcccggacc
    19441 tgcaggcaaa cgcgctggca ttactgggcg agtcgctgaa gcgccgccag atgtggcggc
    19501 cggccatcga ggcgttcaag gccagtcttg ccctcgagga cgttccgcaa gtccgtgaag
    19561 tctacgaggc gctgaggagc gagcacggct tccggctcgt cgactacagc gtcgagcagg
    19621 atttagcggc cccgcgcgtg tgcctgcagt tctccgagaa tctgcaacgc ggccaggatt
    19681 tctcgcagta cgtcgccgtc gacggccagg atgcccaggc gctggtgccc gagggcaagc
    19741 agctttgcat cgaaggcctg tcgcatggtc ggcgctacca ggtgctcgtg cgcgcaggcc
    19801 tgccgtccga ggtcgctgaa gttctcgaaa atcaggctga gcttgcaatt tatgtccgcg
    19861 accgctctcc gtccgtacgc ttcacggatc gcagctatgt gctgccgagc cgcgggcaat
    19921 caggcatccc agttatatcg gtgaacactg agaaggtggc catcgaggtc tatcggatcg
    19981 gggaccgcag cctcatctct gcgctgatga gtggcgacct gcagcggcag ctctcgagct
    20041 acgacctgga tcagatgcgc gagacgtccg gcgcgcgggt ctatcaaggc gagctcgccg
    20101 tcgcccatcg gctgaatgaa gaggtgacga cggctgtgcc ggtcggcgag gcggtgccgg
    20161 agctgaagcc cggcgtctac gccatgatcg ccatgcccgc caattcgcgg gatcaatacg
    20221 gcccacatgc aacccaatgg ttcatcgttt ccgacctcgg catcgtgacg ttcacgggcg
    20281 acgatggcat ccatgcattc gtccgatcgc tcgcgaccac gcagcccctg ccgaacgtca
    20341 gcgtgcggct tgtggcgcgc aacaacgaag tgctcggcac ggctacgagc gatgcccggg
    20401 gctacgtccg cttcgaagcc gggctcacgc gtggtgaagg cggagctgcg ccggcccttc
    20461 tggtggcgga agcgaagaac ggtgactacg cctttctcga cctgacttca tctgccttcg
    20521 acctgaccga ccgcggcgtg aaggggcgcg agcctcctgg tccgatcgac gcttatctgt
    20581 tcaccgagcg cggtgtctac cggccgggcg aggacgtgca tctgaccgcg ctggtgcgtg
    20641 atcgcgcggg ccaggcctca agcgtgccgg taacgctcgt catcacgcgc cctgatgggg
    20701 tcgagcacac ccgtatcgcc ctgaccaatg acgagctcgg cgggcgcacg acgacgttgc
    20761 ggctcgcccc gtcgtcgatg acgggcacgt ggcgcgcgcg cttgcatgcc gacccgaaag
    20821 acgatccgtt ggccaacgtg gcgttcctgg tcgaggactt tgtgcccgag cgcctggacc
    20881 tcgacatcga tcccgtttcg aaggtcctgg caccggaaga aaccggcatc gtgaagattg
    20941 ccgggcgcta tctctacggt gcaccggccg ccaatctggc cgtcgagggc gacatcgtcg
    21001 tgaagcctgc agcggacggg ctgagcgctt ttcctggcta tcacttcggc ctcgcggatg
    21061 aagcagtgga gcccgtccgc gcaccaatcg aaaacctgac gaagacggat gccgaaggac
    21121 gcgccgagct gagcctcatg ctgccgccgg ttccacgcag cgcccggcct ctgcaggccg
    21181 agctgctgct gcgcttgcgc gagcctggcg gccgggccat cgagcgcagc gtcgcactcc
    21241 ctgttgatct caggattgcc cgcctcggtc tgaaaccgct gttccgtgat ctgcagttgc
    21301 gggagaattc gacggcggag ttcgacgtca ttctggttgg cagtgatggc cgccgcgcaa
    21361 gcgttcgtgg cgcgaaatgg gagctggtgc gcctggagca acgctggcaa tggtatagcc
    21421 gcgatggcta ttggacctac gagccgatga cgctcacgcg caaagtggct gccggcgtgg
    21481 tcgacatcgg cgtagacgaa cccgcccgcg tcagcgccaa ggtggattgg ggccgttatc
    21541 gcctggaggt ggcctcgccc gagccgggcg gccctgtgtc caacatcgtc ttcacggcgg
    21601 gctggtacgc ggacgaaggc gccgatagtc ccgaggtgct ggagcttgcg ctcgacaagc
    21661 cgacctacac ggcgggagag atcgcaaaac tgaaaatcgc cgaccggcag ggcgggcggg
    21721 tgctcgtctc ggtgctgggc gaaggtctgc tcgcgagtca ggagatggag ctgcctgccg
    21781 gcggcggcga ggtttcgatc cccgtcgagc gcacgtgggg gccaggcgct tacgtcaccg
    21841 ccatgctcta ccggcctatg gacgagaagg ccaagcgcat gccgagccgt gcgatcggcg
    21901 tgaaatggct tcccatcgac cagagcgcac ggacactgag cgtcgctctc gaagcgccag
    21961 cccaggtccg ctccgggacc acattaagcg tgcccgtcaa gatcactggc ctgagcgagg
    22021 gggaggaagc acgcgtgacc gtggctgcgg tcgaccttgg catcctgaac ctgacccgct
    22081 tcgaggcacc tgcgcccgaa ggctggttct acggccaacg tcgcctcggc accgagatcc
    22141 gcgactacta cggccgcctc atcgacggca tgcgggccga acgcggccgc ctgcgctccg
    22201 gaggcgacga gggcggcggc cttgccatgc agggcagccc gcctgtcgag gagatcgtgt
    22261 cgctgttctc cggcatcgtt cg
//
LOCUS       DASVDU010000248         9888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_526939, whole genome shotgun sequence.
ACCESSION   DASVDU010000248 DASVDU010000000
VERSION     DASVDU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526939"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..351)
                     /locus_tag="VF226_16255"
     CDS             complement(148..351)
                     /locus_tag="VF226_16255"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7075592.1"
                     /translation="MDEIEAAFDRVTSNPLHYQEVEPDIRRAVVHTFPYLVFYTFDEE
                     SVHVLAVIHGAQDPAYINERLGA"
     gene            complement(837..1769)
                     /locus_tag="VF226_16260"
     CDS             complement(837..1769)
                     /locus_tag="VF226_16260"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX7075593.1"
                     /translation="MTLILTLGCSDYIVQASDRRLVADGHLVDDESNKSGVLECMNAR
                     LAFGFTGLAKFGSFDTRRWLLDALHDAGPPDYAAKGIIDRLADKASRAFAIAPLNGIP
                     SQHKRLSIMFSGYLYHHSPPRMGYAIVTNYQDFIAGIERPGAKEQFHVQYWQERRPLD
                     DDVTLVQRIGTWRAMTREDEVQLRALLKARRPARALTGKAVDVMQSMADRASANGAIG
                     KQISTIQLPVSRGEPVQSGYYTRKLGYVSYMADYVIATSNENRTVMRDMEIRQLRGDT
                     PIAVAVPKVGRNVPCPCGSGRKYKFCHGHPRLLN"
     gene            1884..2384
                     /locus_tag="VF226_16265"
     CDS             1884..2384
                     /locus_tag="VF226_16265"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7075594.1"
                     /translation="MRGESSGQHEAFSYVSLEERIPADHPLRTIRARAEAALKRLSPV
                     FKEMYARGGRPSIPPERLLKAQLLIALYSVRSDRQFCESLSWNLLHRWFLGMTMDEPG
                     FVASTFSKNRQRLLEHEVAPRFFAEVVRQARAEDLLSEVSSFGVMEPNGFRHIGASRF
                     SAYWSQ"
     gene            2483..3901
                     /gene="istA"
                     /locus_tag="VF226_16270"
     CDS             2483..3901
                     /gene="istA"
                     /locus_tag="VF226_16270"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7075595.1"
                     /translation="MYQYRQVLVRLRQGDGDRDIARAKLMGRAKVARFRALAAARGWL
                     TEGERLPDDAEIAAAVGQARRARSTISSVEPYRALVERWRAQGVAGVAIHAALCREHG
                     YTGSYSSVHRLIAAIERTRPPKATVPLSFAPGEAAQVDFGAGPLLVDPADGRTKRTWC
                     FVMTLCFSRHQYAEFVFDQKVATWLGLHRRAFEWFGAVPGRLIIDNPRCAITRACSRD
                     PIVQRAYAECAEGYAFRIDPCPPADPAKKGIVEAGVKYVKGNFLPTRRFRDLADLNAQ
                     AREWVLTEAGERVHGTTRERPLERFTLEKPLMRPLPDIAPDLGVWARVRVHRDCHVQF
                     ERALYSVPFTLIGRELWLRATDTAVSVYQDHRLIALHLRARSAGTRRTVAAHLPPAAR
                     AFFTHDRQWCLAQAEKIGRRAWSSSARCSPIASSSGCGPPRRPASRRAVRSAAPGSGL
                     RPGARARFAALPYRQDDPRPRL"
     gene            complement(3950..4168)
                     /locus_tag="VF226_16275"
     CDS             complement(3950..4168)
                     /locus_tag="VF226_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075596.1"
                     /translation="MDKKASRRRHRAFELVSRKIDGAGSRRSDDLDVPLTLGTGKGEA
                     IALRDAHDGGSSTANSVPASRTKHAWAA"
     gene            4196..4981
                     /gene="istB"
                     /locus_tag="VF226_16280"
     CDS             4196..4981
                     /gene="istB"
                     /locus_tag="VF226_16280"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7075597.1"
                     /translation="MNPVPELSPHLKQLRLSGILDSLEARNRQAIESKLAYTEFLSLL
                     IQDEVARREQKKFALRLRRAAFRTTKTLEQFDFERLPKLNRALVHELATGRYLAERAP
                     VLIVGPCGTGKSHLAQALGHCAIRQGVDVLFTTCAQLTASLNAARAIGGYDRKLAALA
                     RVPLLCIDDFGLKPLRPPADEDLHDLIAERYEQTSTLVTSNLDFSEWDQAFPANRLLA
                     SATLDRLRHNAYCLVLDGESFRAPKLAPPSSKRLANGGKNAQA"
     gene            complement(4978..5391)
                     /locus_tag="VF226_16285"
     CDS             complement(4978..5391)
                     /locus_tag="VF226_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075598.1"
                     /translation="MRQLPQGSRPLTGQDVVHREIDNQPAAVLRHGIAREIELGGRRP
                     ALPEQPRLRVGNRGMRGVTPALAVEIHALVVRIDRSAVVIVLAAYAFERSQRLDQGPV
                     DREVLLLSLLGINEPGFFGVIEPIFGVRHGHPGVV"
     gene            5409..5735
                     /locus_tag="VF226_16290"
     CDS             5409..5735
                     /locus_tag="VF226_16290"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX7075599.1"
                     /translation="MQTVAGDKGYCTKDFVATLRERGIRPHIAFIDDGRRVPGLDHRT
                     MRHANYQLSQRKRKLIEQAFGWLKAIAGLRKSRFFGRARTELYALMAMSAYNLTRMAR
                     LRPLPT"
     gene            complement(5967..6197)
                     /locus_tag="VF226_16295"
     CDS             complement(5967..6197)
                     /locus_tag="VF226_16295"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEX7075600.1"
                     /translation="MSLSELDTVVLKTDLPQHGLRKGDVGAVVEVYGRAAVEVEFVTA
                     SGHTQALITLSMDQVRPVGPKDILAVRQLDAA"
     gene            complement(6670..6963)
                     /locus_tag="VF226_16300"
     CDS             complement(6670..6963)
                     /locus_tag="VF226_16300"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7075601.1"
                     /translation="MTEIEFRPAAAKDLEEAAEWYEAKQRGLGEEFLEAVRVASNAIV
                     ENPEQFPVLYRNTRRALVRRFPYGIYFRVVSNRALVVACMHGRRRPTRWRSRK"
     gene            complement(6960..7181)
                     /locus_tag="VF226_16305"
     CDS             complement(6960..7181)
                     /locus_tag="VF226_16305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX7075602.1"
                     /translation="MSTRAIDIEKLDAEQKLRLIEELWDSLNDDPSQVPLTQAHREEL
                     DRRLDEIEEGDDAGIPWDEVLSRIRKRLA"
     gene            complement(7355..7654)
                     /locus_tag="VF226_16310"
     CDS             complement(7355..7654)
                     /locus_tag="VF226_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7075603.1"
                     /translation="MGKKNVGSAFDDFLKEEGLFEEVTAVAIKRFIAYQLSEKMSEDN
                     LSKTEMARRMGTSRSALDRLLDPENASVTLQTLQSAARALGGSLKVELDFHRRAA"
     gene            complement(7657..8001)
                     /locus_tag="VF226_16315"
     CDS             complement(7657..8001)
                     /locus_tag="VF226_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7075604.1"
                     /translation="MSADRPLPVVFFRLDSGREPVREWLRSLDGSSRRSIGEDIKTLQ
                     FGWPIGMPLAQKMDRELWELRSTIPSGIARTFFTVHDARIVLLHGFVKKSQKTPAKEL
                     ALAKRRLTKLRS"
     gene            complement(8184..8465)
                     /locus_tag="VF226_16320"
     CDS             complement(8184..8465)
                     /locus_tag="VF226_16320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075605.1"
                     /translation="MTTSLEWFEVHLTTPKRFNRSTSKGYYRRNTKGIAWFRDTAKEH
                     ISGMYKIKRIAEANGHSVDVIREERVGYVVYEDEFQVIAEPYADTRTAP"
     gene            complement(8694..8924)
                     /locus_tag="VF226_16325"
     CDS             complement(8694..8924)
                     /locus_tag="VF226_16325"
                     /inference="COORDINATES: protein motif:HMM:NF027602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4926 domain-containing protein"
                     /protein_id="HEX7075606.1"
                     /translation="MCISELDTVVLKADLPHHGLKKGDVGAVVEVYAPDAFEVEFVTA
                     SGHTQALVTLGTDKIRPVGRKDILAVRQLDAA"
     gene            complement(8927..9259)
                     /locus_tag="VF226_16330"
     CDS             complement(8927..9259)
                     /locus_tag="VF226_16330"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075607.1"
                     /translation="MKLSHIESATIPPEKVRDYLLSQNHAIDRYKTVFFRSLGCDQAN
                     WEALADDLRSLLTSDAAQIGITEYGVKYEIRGAITGPKGRTANVITAWIILSGEDAPR
                     FVTAYPED"
     gene            complement(9389..>9888)
                     /locus_tag="VF226_16335"
     CDS             complement(9389..>9888)
                     /locus_tag="VF226_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075608.1"
                     /translation="RFAAFSLRRFAAYFLLGRRRRMSRSALVAAMVLASACTVFPSAE
                     DVVIVGRVLTVEGEPIPNVTLYYVDLKYRAFSMPGVGASGYVKTNESGEYRIELDEIH
                     DALDIGVNDDGCGGIDGTYALIEKGRFADGSTVVQNLRFCAVSRKQSAKQRHGSRKLG
                     SANAA"
BASE COUNT         1946 a         3065 c         2872 g         2005 t
ORIGIN      
        1 attctcaatc ttgccaacga tctcaaactt cacagcggat cgcgggcgag cagcctaacg
       61 accaagctca gcggcaaagg caagctggcg cggcccttgc gtacgcaagg ggcgtgacag
      121 cttgacgtgt ccgactgcag cgcattgtta ggcgccaagc cgctcattga tgtaggccgg
      181 atcttgagcc ccatggataa ccgccaatac atgcacggat tcctcgtcaa aggtgtaaaa
      241 caccaggtac gggaaggtat ggactacggc cctccggatg tccggctcta cttcttgata
      301 atggagtgga ttgctcgtga cgcgatcgaa agccgcctcg atctcgtcca agtacctcaa
      361 tcccagtccc cgttgctttc ttcgtaggat cgatatccat ggaggatatc cgcttctgct
      421 gccttcgtaa caatgagcgc acggctcatt cggaaagtat tctgtttctg acctcagccc
      481 acggggagcc ggcactcgga tttgctcgga aagcggccag tctcttccga agaaggcgac
      541 gtgtcgcgtc ggaaagctca atctgttccg ggcactccgc gatgctgtcc cagatttcgg
      601 caaccagctg aattcgctca gaaaccggca gttctaatgt gtcagcagct gagaatttca
      661 ttgcatgaaa ctaccacagg gcgccccatt ccgccacgag ccaaatgccg cagaaggggt
      721 cgaatttggc gcctaacgac caagctcagc ggcgcgtcaa gctggcgcga cccttgcgaa
      781 tgcaaggggc gtgacagttt gatgcgtccg tctgcagcgc cttgttaggc ggcgcgtcaa
      841 ttgagtagcc gtggatggcc atgacagaac ttatacttcc ttccgctacc gcacgggcat
      901 gggacattcc gtcctacttt tggaactgca acggctattg gcgtgtcccc tcggagctgt
      961 cggatctcca tgtcacgcat gacagttcta ttctcgttcg aggtggcaat aacgtaatcg
     1021 gccatgtacg acacgtagcc caacttgcga gtgtagtacc cactttgtac cggctcgcct
     1081 cgacttaccg gtagttgaat cgtcgatatc tgcttgccga tagctccatt agcgctggct
     1141 ctgtctgcca tactttgcat cacatcaacc gccttcccgg taagtgcacg ggcgggcctt
     1201 ctggccttca aaagtgcacg gagctgtacc tcatcttcgc gtgtcatggc ccgccaggtg
     1261 ccgatacgct gaactaacgt aacatcgtca tccaacggac ggcgctcctg ccaatactga
     1321 acgtggaatt gttccttcgc tccaggtctc tcgatgcccg caatgaagtc ttggtaatta
     1381 gtcactatcg cgtagcccat ccgcggaggg ctgtggtgat agaggtagcc cgagaacatg
     1441 atcgacagac gcttgtgctg cgagggtatg ccatttaacg gggctatcgc aaaggcgcgc
     1501 gatgccttat ccgccaaacg atctataatc ccttttgccg cgtagtctgg aggacctgca
     1561 tcatgaaggg cgtccagtaa ccagcgccga gtgtcgaaag acccaaattt ggcgagccca
     1621 gtaaacccga aggcaagcct tgcgttcatg cactctagca cgccagactt gttcgattcg
     1681 tcgtctacaa gatgaccatc ggcgaccaag cggcgatcgg acgcctgcac gatgtagtca
     1741 ctgcatccga gcgttaagat cagggtcatt cttgtgcaac agcctttgta tgcggaatcc
     1801 cgccgtgccg cctaacaggc cgctgaaaaa gtcgtcattt gatgacgtat gctacgcctg
     1861 ttggtgcgac gaggggattg gcgatgcggg gcgagtcgag tggtcagcat gaagcgttct
     1921 cgtatgtgag tctggaggag cggattccgg cggatcaccc gctgcggacg atccgcgcaa
     1981 gagccgaggc ggcactgaag cggctgtcgc cggtgttcaa ggagatgtat gcgcgcggtg
     2041 gccgtccttc gattccgccg gaacggctgc tgaaagcgca gctgctgatc gccctgtatt
     2101 cggtccgttc ggatcggcag ttctgcgaga gtctgtcctg gaacctgctg catcgttggt
     2161 ttctcggcat gacgatggat gagccgggtt tcgtggcctc gaccttcagc aaaaaccggc
     2221 agcggctgct cgagcatgag gtggcgccgc ggttcttcgc ggaagtggtg cgccaggcca
     2281 gggcagagga tctgttgtcg gaggtgtcat ctttcggcgt aatggagcca aacggttttc
     2341 ggcatattgg agccagccgg ttttcggcat attggagcca gtgaaggggt gagcgcgggc
     2401 atctcggatt gacggcggca gccgctaccc tcaaggcctc ttcggtcttg gggggcgggc
     2461 ggtgggtcgc aggagattcg agatgtatca gtaccggcag gtcctggtgc ggctgcgcca
     2521 gggagacggc gatcgggaca tcgcccgggc gaagctgatg ggccgcgcca aggtggcgag
     2581 gttccgggcg ctggccgccg cgcgagggtg gctcacggag ggcgagcggc tgcccgacga
     2641 tgccgagatt gccgcggccg ttggccaggc ccggcgagcc cgatcgacga tttccagcgt
     2701 cgagccttac cgcgcgctgg ttgagcgctg gcgcgcacaa ggggtggccg gggtggcgat
     2761 ccacgcggcg ctgtgccgcg aacacggcta caccggcagc tattcctcgg tgcaccggct
     2821 gattgccgcg atcgagcgga cccgcccgcc gaaggcgacg gtgccgttgt cgttcgcgcc
     2881 gggcgaggca gcgcaggtcg atttcggtgc cggcccactg ctcgtcgatc ccgccgacgg
     2941 gcgcacgaag cgcacctggt gcttcgtgat gacgctgtgc ttctcccggc atcagtacgc
     3001 ggagttcgtc ttcgaccaga aggtcgcgac ctggctcggg ctgcaccgac gggccttcga
     3061 gtggttcggc gcggttcccg ggcggctcat catcgacaac ccccgctgcg cgatcacccg
     3121 cgcctgcagc cgtgacccga ttgtgcagcg cgcctacgcc gagtgcgccg agggctacgc
     3181 ctttcgcatc gatccgtgtc cgccggcgga tccggcgaag aagggcatcg tcgaggcggg
     3241 cgtgaagtac gtgaagggca acttcctgcc gacccgccgc ttccgcgacc tcgccgacct
     3301 caacgctcag gcgcgcgagt gggttttgac cgaagccggc gaacgcgtcc acggcacgac
     3361 gcgcgagcga cccctcgaac gcttcaccct cgagaagccg ctgatgcgcc cgctgcccga
     3421 tatcgcgccg gacctcggtg tctgggcccg ggtgcgggtg catcgcgatt gccacgtgca
     3481 gttcgagcgg gccctctact cggtgccgtt cacgctcatc gggcgggagc tctggctgcg
     3541 cgccacggat accgccgtga gcgtgtacca agaccatcgc ctgatcgcgc tgcacctgcg
     3601 cgcccgttcg gccggcacgc gccgcaccgt cgctgcgcac ctgccgccgg cggcgcgggc
     3661 cttcttcacc cacgatcgac agtggtgcct cgcgcaggcc gagaagatcg gccggcgtgc
     3721 ctggagctca tccgcacgct gttcgccgat cgcatcctcg agcggctgcg ggccgcccag
     3781 gcgtcctgcg tctcgccgag cagttcgatc cgctgcgcct ggaagcggct tgcgcccggg
     3841 cgctcgcgca cgcttcgccg cactaccgta ccgtcaagac gatcctcgcc cgcggctttg
     3901 accgacgaga cgatccacac accctgccgg gggcacatgc cattggcgcc tatgccgccc
     3961 aggcgtgctt cgtgcgcgat gccggcacgc tgttcgccgt cgaggagccg ccgtcgtgag
     4021 cgtcccgaag cgcgatcgcc tcgcctttgc cggtacccaa ggtcaagggg acgtcgagat
     4081 cgtcgctcct gcgcgatcct gcgccgtcga ttttccgcga gacgagttcg aacgcccggt
     4141 ggcggcgtcg gctcgccttc ttatccatcc tgatgccacc gaaggagatg ctctcatgaa
     4201 ccctgtccct gagctcagcc cacacctgaa gcagctgcgc ctgtccggga tcctcgactc
     4261 gctcgaggcg cggaaccgtc aagccatcga gtccaagctc gcctacaccg agttcctctc
     4321 cctcctcatc caggacgagg tggcgcgacg cgagcagaag aagttcgcct tgcggctgcg
     4381 gcgcgccgcc ttccgtacca ccaagaccct cgagcagttc gacttcgaac gcctccccaa
     4441 gctcaaccgt gccctcgtgc acgagctcgc caccggccgc tatctggcgg aacgcgcccc
     4501 ggtgctcatc gtcggtccct gcggcaccgg caagtcgcac ctcgcccagg cgctcgggca
     4561 ctgcgccatc cgccagggcg tcgacgtgct cttcaccacc tgcgctcagc tcaccgccag
     4621 cttgaacgcc gcccgcgcga tcggcggcta cgatcgcaag ctcgccgctc ttgcacgcgt
     4681 gccgttgctg tgcatcgacg acttcggcct caagcccctg cggccgccgg ccgacgagga
     4741 cctgcacgac ctcatcgccg aacgttacga gcagaccagc acgctcgtga ccagcaatct
     4801 cgacttcagt gaatgggatc aggccttccc ggccaatcgg ctcctcgcct cggccaccct
     4861 cgatcggctg cgccacaatg cctattgcct cgtgctcgac ggcgagtcgt tccgcgcgcc
     4921 gaagctggcg cccccgagct cgaagcgcct tgccaacgga gggaagaacg ctcaggctta
     4981 aaccacgccg ggatgcccgt gcctcacacc aaaaatcggc tcgattacac cgaaaaatcc
     5041 tggctcattt atgccgagaa gtgacaggag gagcacttca cggtcgacgg gaccctgatc
     5101 gagtcgctgg cttcgctcaa aagcgtacgc cgcaaggacg atgacgacgg cggaccgatc
     5161 gatccggaca accagggcgt ggatttccac ggcgagcgcc ggagtaacgc cacgcatgcc
     5221 tcgattaccg acccggaggc gcggctgttc cggaagagcc gggcggcggc caccaagctc
     5281 tatttctcgg gcaatgccgt ggcggagaac cgctgcgggc tgattgtcga tttctcggtg
     5341 gacgacatcc tgaccggtga gcgggcggct gccctgaggc agctgacgca cgtcgcaagc
     5401 atcggcacat ccagaccgtg gccggtgaca agggatactg caccaaggac ttcgtcgcga
     5461 ccctccgcga gcgtggcatt cgtccgcaca ttgcgttcat cgacgacggc cgacgggttc
     5521 cagggctcga tcaccgcacg atgcgtcatg ccaactacca gctgtcgcaa cgcaagcgca
     5581 aactcatcga gcaggcgttc ggctggctca aagcgatcgc gggattgaga aagagccgct
     5641 tcttcggccg cgcacgcacc gagttgtacg cactgatggc gatgagcgcc tataacctga
     5701 cccgaatggc ccgattgcgg ccgttgccga cctgagtgtg agatctcgac atgtccgcca
     5761 cgccaacgag ccgatctgaa accgggaaag cactcttcag cccttcgcca acgctcgtct
     5821 cgtcaccaat ttcagactcg aatgccattt cgagccgttt ttcagcggcc tgctaacatc
     5881 caagctcagc ggcaaaggca agcgggcgtg gcttttgcca atgcaaaagg catgacagct
     5941 tgactttgtc cgtctgcagc gccgtgttag gccgcatcaa gctggcgaac ggccagaata
     6001 tccttcggtc cgaccggacg aacctggtcc atactaaggg ttatcagtgc ctgggtatga
     6061 cctgaggcgg ttacaaactc cacctccaca gcagcgcggc cgtatacctc gacaacagca
     6121 ccgacgtcgc ctttcctcag gccgtgctgc gggaggtcag ttttcagcac tacagtatca
     6181 agttcactaa ggctcatggg gctagtcctc cggatacgcg gtgacgaatc tcggcacttc
     6241 ctcaccggtc agtataatcc aagcggacag gacgttagca gtctgaccgt tcgggccagt
     6301 gatcaagcca cgaatctcgt atttgacgcc gtattctgta atgtcgatct gcttagcatc
     6361 tcccttcaac aactcctgta gggatatttg cgagagcttc ccagttggct tggtcgtacc
     6421 cgagagaccg gaagaaggca gctttgtagc ggccgatcgg atggctcgga gacagaagat
     6481 aatctcttac cttactggcc ggaaccgtcg cggtttcgat gtttgcgagt ttcacggcgc
     6541 tggaatagtg tcggcggtgg gatgcggcct aacaaccaag ctcagcggca aaggcaagct
     6601 ggcgtggctt ttgcgaatgc aaaagacatg acagattgtc tttgtccgtc tgcagcgcct
     6661 tgttatacgt catttgcgcg aacgccatcg ggtaggtcgc cgccgaccgt gcatgcatgc
     6721 gacaaccagt gctcgattgg acacgacgcg aaagtagatc ccgtatggga agcgccggac
     6781 cagcgcgcgg cgcgtattcc tatacagcac cgggaactgc tcaggatttt ccacaatcgc
     6841 gttcgaagcg acccgaactg cttccagaaa ttcctcgcct agaccgcgct gtttggcttc
     6901 gtaccattca gctgcctctt ccaagtcttt ggcagccgca ggccgaaact caatttcggt
     6961 cacgcgaggc gtttccggat ccggctcaga acctcatccc aaggtattcc ggcgtcgtcc
     7021 ccttcttcga tttcatcgag ccgccgatcc agctcttctc tatgggcctg ggtaagggga
     7081 acctgagacg gatcgtcatt caggctatcc caaagctcct caatgaggcg cagcttctgc
     7141 tcagcgtcca gcttctcaat atcgatggcg cgggtgctca ttgccgggat tatagcgccc
     7201 ctgcacgctg ccacgaacct cgaaatacgc ggttttcctt gagaaggacg tataacgact
     7261 aagctcagcg gcaaaggcaa gctggcgtgg cttttgcgaa tgcaaaaggc atgacagctt
     7321 gactttgtcc gtctgcagcg ccttgttaga cgacttatgc tgcacggcga tggaaatcaa
     7381 gctccacctt gagggaaccg cccagtgccc gtgcagcgct ctgcaacgtc tgcaatgtga
     7441 ccgaggcatt ctcaggatct aaaagacggt ccaaagccga tctgctcgtc cccatgcggc
     7501 gagccatctc cgttttgctc agattatctt cagacatttt ctccgatagt tgataggcaa
     7561 tgaaccgttt gatggccacc gccgtcacct cttcgaagag accttcctct ttgagaaaat
     7621 catcaaaggc actacctacg tttttcttac ccatctctat gatctcaact ttgtcagacg
     7681 ccgcttggca agcgccaact ccttcgccgg tgtcttctga gacttcttca cgaacccgtg
     7741 cagaaggacg atcctggcat catgaaccgt gaagaatgtc cgcgcgatgc ccgatggaat
     7801 cgttgagcgc agctcccaga gttcgcgatc catcttttga gctaatggca tcccgatcgg
     7861 ccagccgaac tgcagcgtct tgatgtcctc gccaattgaa cgccggctgc taccgtcgag
     7921 acttctaagc cactcacgaa ccggttcacg tcctgagtcg agccgaaaga agacgacggg
     7981 tagcggcctg tctgcggaca tagccatatc gtaccatttt cggtacggag gtcaaagctt
     8041 ggatttgagg tggggaagtc gtctaacaac caagctcagc ggcaaaggca agctggcgcg
     8101 gcttttgcga atgcaaaagg cgtgacagtt tgactttgtc cgtctgcagc gccttgttag
     8161 acggcacgaa caacggcata tccctaagga gcggttcgtg tatccgcata cggttctgcg
     8221 atcacctgga actcatcctc atacacgacg tatccaaccc tttcttctcg aatgacatcc
     8281 acggaatgtc cgttcgcttc cgcgatccgc ttgatcttgt acatgccgga aatgtgctcc
     8341 ttcgcagtat cgcggaacca ggctatcccc ttggtgttgc gacgatagta ccccttcgag
     8401 gtggatcggt tgaagcgctt cggcgtggtc agatgcacct cgaaccattc aagagaagtc
     8461 gtcaaagcct gccgatgctc ctccgagatt gtgtcagcgt cgcggagagc gtaggcaaga
     8521 gtgaacaggc cgtcgtttgc tccagaactc ggatgcctct gcgggacggt gaatctgacg
     8581 aaagccacgg ctttgccgtc taacaaccaa gctcagcggc aaaggcaagc tggcgcggct
     8641 tttgcgaatg caaaagacgt gacagcttga ctttgtccgc ctgcagcgcc ttgttaggcc
     8701 gcgtcaagct gacgaacggc aagaatgtcc ttgcgcccga ctggacggat cttatccgtg
     8761 cctagagtaa ccaatgcctg ggtatgacca gaggcagtga caaactccac ttcgaatgcg
     8821 tccggtgcgt aaacttcgac aacggcaccg acgtctcctt tcttcaagcc gtggtgaggg
     8881 agatcagcct tcagcactac agtatcgagt tcactaatgc acatgactag tcctcgggat
     8941 acgctgttac aaatcttggg gcgtcctccc cgctcagtat aatccaagcg gttatgacat
     9001 tcgcagttcg gcccttcggg cccgtaatcg cgccgcgaat ctcgtatttg acgccgtatt
     9061 ctgtaatgcc gatttgcgcc gcatcgctcg tcagcaacga ccgcagatcg tcagcaagcg
     9121 cttcccagtt ggcctggtcg cacccgagcg accggaagaa cacggtttta taccggtcga
     9181 ttgcgtgatt ctgtgacaga aggtagtccc tcaccttctc gggtggaatg gtcgccgatt
     9241 cgatgtgcga gagtttcaca gcgacgaatt ctgctccgag ggtgggtacg cggcctaacg
     9301 accaagctca gcggcaaagg caagctggcg cggctcttgc gaatgcaaga ggcgtgacag
     9361 cttgtctttg tccgtctgca gcgccttgtt aggccgcatt tgcactgccc agtttgcgtg
     9421 acccgtgcct ttgcttagca gattgcttcc gcgacaccgc gcagaaccta agattctgca
     9481 caaccgtcga accgtctgcg aagcgaccct tctcaatgag agcataagtt ccgtcgatgc
     9541 cgccgcagcc atcatcattg accccaatat ctagcgcgtc gtgaatctcg tctagctcta
     9601 tccggtattc tccagattca ttggtcttta cgtagccaga tgcaccaacg ccaggcatcg
     9661 agaatgcgcg atacttcaga tccacatagt agagagtcac attgggaatc ggttcgcctt
     9721 caacggtcaa tacgcgccca acgattacaa cgtcctcggc agacggaaaa accgtgcacg
     9781 cggaggcgag taccatcgcc gcaaccaagg ctgatcgaga catgcggcgc ctgcggccta
     9841 acaaaaagta ggcggcgaaa cgccgcagag aaaatgcggc aaaacgct
//
LOCUS       DASVDU010000249        25080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_444547, whole genome shotgun sequence.
ACCESSION   DASVDU010000249 DASVDU010000000
VERSION     DASVDU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 25080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25080
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444547"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1304)
                     /gene="fumC"
                     /locus_tag="VF226_16340"
     CDS             complement(<1..1304)
                     /gene="fumC"
                     /locus_tag="VF226_16340"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX7075609.1"
                     /translation="MTSADVRIETDTFGPIEVPADRYWGAQSQRSLGNFKIGWEKQPK
                     PIIRALGIVKRAAAETNMALGRLDPKIGEAIVKAAQEVIDGKLDEHFPLVVWQTGSGT
                     QTNMNANEVISNRAIEMLGGEKGSKKPVHPNDHVNMSQSSNDTYPTAMHIAAAEQVHH
                     ELLPALHHLHGALDAKAKAWAHIIKIGRTHTQDATPLTLGQEFSGYAKQVENGIKRIE
                     MTMPMLMELAQGGTAVGTGLNAPKGFAEKVAERIAAITGLPFTSAPNKFEALAAHDAM
                     VMTHGAINTVAVSLFKIANDIRFLGSGPRSGLGELALPENEPGSSIMPGKVNPTQCEA
                     LTMVCAQVFGNHTTMTVAGSQGHFQLNVFNPVMAYNFLQSVRLLADSARSFTDNCVVG
                     IQPREDNIKAALERSLMLVTALAPKIGYDNAAKIAKAAHKKGT"
     gene            1508..2632
                     /locus_tag="VF226_16345"
     CDS             1508..2632
                     /locus_tag="VF226_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX7075610.1"
                     /translation="MLQQWIDFHIYRFLAATNWSELSVFFIFILAIAFGLVVHAAIMW
                     LVRKFFPVKSDEVGGRILRRISRLTRLALILLAIKIALPAARFEPGIQQAALRVLDLG
                     LVALIGLALIAVINIGWDVVESHNRLDVEDNLRARKLRTQIGVLRRTAVIIIAILTCG
                     GMMMTFPSLREYGLSLLASAGAAGLVVGLSARPLLSNLLAGVQIALTQPIRIDDSVIV
                     EGEFGRVEEITTTYVVIRTWDWRRIVMPLSSFLEKPFQNWTLEDSSLIGSVFWHVDYS
                     APIEEMRRKLNDLLKESAYWDRRVAALQVVEAEERTLKIRALMSARNAAAAWDLRCQI
                     REQMITWLQKEHPNALPRVRLEEPHRVRANGTYAEIAQAE"
     gene            2873..3121
                     /locus_tag="VF226_16350"
     CDS             2873..3121
                     /locus_tag="VF226_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075611.1"
                     /translation="MTPKIVVMIIMLAMPNGDSGVHVKPYATAETCIEAANIEATDPF
                     VHSVECAELEDGLLTLRFDRPDVPESPPVTTGALDSAE"
     gene            complement(3158..3352)
                     /locus_tag="VF226_16355"
     CDS             complement(3158..3352)
                     /locus_tag="VF226_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075612.1"
                     /translation="MLRNGNSDNGISLLGLLLAVVIVLGLLWYIFGRQEAPTTTATPG
                     DGVEVPTTPPTTPITPRAPQ"
     gene            complement(3433..3759)
                     /locus_tag="VF226_16360"
     CDS             complement(3433..3759)
                     /locus_tag="VF226_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7075613.1"
                     /translation="MIYELRVYDCVPGRLPALLNRFENTTLKIWERHGIHQAGFWTTL
                     VGESNQQLNYLLRWESMAEREQKWNAFMADPEWQQARAASEKDGPIIAKVSNQFWQPT
                     SFSAVK"
     gene            complement(3859..4638)
                     /locus_tag="VF226_16365"
     CDS             complement(3859..4638)
                     /locus_tag="VF226_16365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075614.1"
                     /translation="MSTQGLFAGERAVVTGSASNIGRGIATALAREGAHVVLVDIDME
                     RNAAAAREIGDAGGSAETISVDLSQPDGWKAILEAVDGRLPEMLVHAACPRRIEGDIP
                     TVVSEATWDAMLNTNVRSGFFLGREIGKRMQEAGVRGRMLYITSLHAYAPRNLAHYSA
                     AKAGMTMVVRELARAFGPSGIRVNALAPGAVPGGGAANMDETFQPKRKIPLQRFGTAA
                     DMAGMAIALLSDRFSGYVTGATVPVDGGLALYNWIPMPGQD"
     gene            complement(4757..5452)
                     /locus_tag="VF226_16370"
     CDS             complement(4757..5452)
                     /locus_tag="VF226_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEX7075615.1"
                     /translation="MIDYEALAQDAMRGVVRAVLARTAKSGLPGDHHFYISFDTQHPG
                     VVLSKRLREKYPEEMTIVLQHRFWDLSVTEDRFEVKLTFDGIPERLVVPFAALKIFFD
                     PSVRYGLQFEAPDLGPEALEQSQSGLETGAEEEEAMPAPRPQPAPLRGAPRPAVERKP
                     TRSPRKARPDRPSDQPGMTEPSSAHPASSKPAPEVPSKSHSQVQQSADETEAERPPSG
                     GAQIVSLDAFRKK"
     gene            complement(5523..5887)
                     /gene="ssrA"
                     /locus_tag="VF226_16375"
     tmRNA           complement(5523..5887)
                     /gene="ssrA"
                     /locus_tag="VF226_16375"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            5910..6680
                     /locus_tag="VF226_16380"
     CDS             5910..6680
                     /locus_tag="VF226_16380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7075616.1"
                     /translation="MLISAIRELLTGQPAYHARSPWRPVPAVAAALIMVAIAAGVATY
                     LVGTLQLAPPIAIPMDLTPADENRIWRMTVWLLSMQASIIALVLVAAGMFGGRRAEVL
                     AFNRDTPKRIYVAAVIVMVIAQVAYNVIVFPFARDTMVEDVQPMLEPLHSDAFWLFAL
                     AIVVGAPLSEELLFRGFLLSALAQSRIGFLGAALVTTLGWTALHAGYSGVGLVEVFLA
                     GLLFSWLLWRTGNLWVPLVCHGFYNGMVLLALVTLPLT"
     gene            6733..7527
                     /locus_tag="VF226_16385"
     CDS             6733..7527
                     /locus_tag="VF226_16385"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX7075617.1"
                     /translation="MDQYLNLMRRVRTEGIRKTDRTGTGTLSVFGHQMRFDLARGFPL
                     VTTKKLHLKSIIHELIWFLRGDTNIAYLKANGVSIWDEWADEHGDLGPIYGKQWRSWA
                     APNGRTIDQIAEAVETLKTNPDSRRIIVTAWNPADIPDMALAPCHCLFQFYVADGRLS
                     CQLYQRSADVFLGVPFNIASYALLTLMMAQVTGLRPGEFIHTFGDAHLYLNHLDQADL
                     QLTRTPRPLPTMRLNPEVKDIFGFRYEDFELTGYNPHPHIKAPVAV"
     gene            7524..8090
                     /locus_tag="VF226_16390"
     CDS             7524..8090
                     /locus_tag="VF226_16390"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX7075618.1"
                     /translation="MSDRPQPDADPVIALVVAAAENDVIGRGGRLPWRIPSDLRLFRR
                     LTMGKPVVMGRKTFQSIGKPLDGRDNIVVSRDPAFDPAGVSVKRTLAEAIAEARTAAA
                     ARGQNEIMVIGGEAVFREALPLARRIYLTRVHGLPEGDTFLPGIPPGEWDEASREDLP
                     RDPTDEFACTLIVLERKPEPVRDTASGS"
     gene            8253..9389
                     /gene="hflK"
                     /locus_tag="VF226_16395"
     CDS             8253..9389
                     /gene="hflK"
                     /locus_tag="VF226_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX7075619.1"
                     /translation="MPWNNQSSGGGGWKSGGGGGPWGSGGGGGPWGQGPGGGNQPDLE
                     EILKRGQDKVKQVMHGSGVPGPLILLLSAVALAVVGFYAFTFTVRPDELGVVLRFGQF
                     HRQEPPGLHFRLPYPIEEVRLPKVTVANRTEIGFRGPTDNRARSIQSQEVLDESLMLT
                     GDENIVDIGFVVLWNIKDAAAYLFNIENPEKTVKEVAESAMREVVGKSRVQDVLTEKR
                     SQIEQEVLDLVQRTLDSYGAGINITQLQMQRAEAPSQVIDAFRDVAAAAIDQRRIENQ
                     AYAYANKVIPEARGEVQRILQEAQAYKERVVAEAQGEADRFLNVLEEYRKAPELMRQR
                     LYLETMERVLSSTDKVIIDSNGGQSVVPYLPLDQLQPRRQQQQQ"
     gene            9401..10339
                     /locus_tag="VF226_16400"
     CDS             9401..10339
                     /locus_tag="VF226_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEX7075620.1"
                     /translation="MRTTLITILVALGLAAVGAYLSMFIVKQHQQALVLEFGKPKRII
                     DTAGLHWKIPVVETVEYFDKRILDLDTSPQELFSSDQNKLIVDSFARYRIVDPLRYFQ
                     TVRNELGVRSRVGPILDSSLRRVLGSASFADAVRDRRAQLMNLIQKQVNAEARNFGIE
                     IVDVRTKRVDLPPQNSASIYERMKTDRQREAAEYRARGEEAARRIRAEADRQVAVMKA
                     EAERDARRLKGEGDAERARISNEAFKQDPEFYQFLRSMEAYETALKRGDTRLVISPES
                     PTFRDFFRFFSESVTPATKVPNVTGTTAPNIALPQR"
     gene            10336..10521
                     /locus_tag="VF226_16405"
     CDS             10336..10521
                     /locus_tag="VF226_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEX7075621.1"
                     /translation="MSDLAVALGLVLVIEGLLWAAAPDLAVRLLNAAAEAPPQALRTA
                     GLTAAAVGVFIVWLVRG"
     gene            10641..12125
                     /locus_tag="VF226_16410"
     CDS             10641..12125
                     /locus_tag="VF226_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX7075622.1"
                     /translation="MTALAQVRDVGIARTLAGLIFSLALVTFAGSSEARGPQSVADVA
                     EELIDAVVNISTTQTLKTPEGTPLPRVPDGSPFEDLFEDFFNKKGTNPHRDRRVSSLG
                     SGFVIDGKEGLIVTNTHVIEGADEIVVNFADGSRLKVDKVVGKDGKTDLALLKVTPKK
                     PLKEVKFGASEAIRVGDWVMAIGNPFGLGGSVTVGIISAKARDINSGPYDDYLQTDAA
                     INKGNSGGPLFNMAGEVIGINTAIISPTGGSIGIGFAVPSDIAMPVIEQLRLYGETRR
                     GWLGVRIQTVSEDVAESLGVPPNTGALVAGISPDSPAAKAGIEAGDVITKFDGKEVTS
                     MRGLPRIVAQTPIGKTVPVEVMRKGEKKTLEVVVGRLSEDDDAAAEEPAPPTTRGETP
                     ANPGQGLIGLKVTPLTDELRRKHGIEKKIKGLLVTEVDQDSPAAEKGVKAGDIIVEAA
                     QEQVATADDLTKSVDKVRKSGRKAVLLRLEDGKGDMRFVAVPID"
     gene            12290..13003
                     /locus_tag="VF226_16415"
     CDS             12290..13003
                     /locus_tag="VF226_16415"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEX7075623.1"
                     /translation="MSENRSLLSTIADSIPPVHRDGHKFVAIGLGITFLFFFLWPPLG
                     WVFAVVTAWVLYFFRDPDRVTPLREGLVIAPADGRISAIERVRPPQELGLGEELRTRI
                     SIFLSVFDVHVNRSPVAGRITRSVYTPGAFFNAELDKASEENERRGLVIMMPSGNEIG
                     VVQIAGLVARRIVTFVGEGDTVGVGQRFGLIRFGSRVDVYLPPGARPLVAVGQRTIAG
                     ETVLADLKSDEPEREARRS"
     gene            13058..13954
                     /locus_tag="VF226_16420"
     CDS             13058..13954
                     /locus_tag="VF226_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEX7075624.1"
                     /translation="MDPLLTRLEEETRRRRRRAIFKYRQVPVRLLIPNFFTLLGLCAG
                     LTAIRMAIESRYELALAAIVFAALLDGVDGRVARMLKASSRFGAEFDSLADFVNFGVA
                     PAIVLFTWGAWETPAMRGLAWIVVLLLAVACGLRLARFNTALDVERPKWQSNYFSGVP
                     APAGAIIALLPVYLDQLGLLQMRIGWPIYFVLAYALLIAVLMVSTIPTFSGKLLGERI
                     SREWVMPIFVLLGGLVALLVTYPFATLSIGTILYLAMIPFSIRRYRSQMLDMEAEERR
                     RPEDNIPKLVVDKPSHPDRMPR"
     gene            14038..15369
                     /locus_tag="VF226_16425"
     CDS             14038..15369
                     /locus_tag="VF226_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7075625.1"
                     /translation="MDANTSAADDVIEILIVLALFLLNGLFALSELAVVSSRHSRLKA
                     MAAAGRRGANRALELATNPSRFLSTVQIGITLVGIVSGAYSGEAFGMRAAQYLSSVGL
                     PTGAAITIGYGLVIVIVTYFSVIIGELVPKTLALRNAEGLACAVAPIMIIVDRMAAPA
                     VWLLDSSTRLVFRVLGLPTEREIRVTDEEIKALIAEAERAGVLETGEREMLSGVMRLA
                     DRSVVGLMTPRTEVDWIDITGSHEEIKERIISTPHSRLPVGEGSVDQMVGVIQTRELL
                     ATLLSGKSFDVRSLIRKAPIIPETTDALDALAILRDAEVPMALIHDEYGNFQGLVTPA
                     DVLEAIAGAFRSDTAGEVYAVEREDGSWLLSGAMPADEMADKLAIKLPEKRAYETVAG
                     FVLAHLQHLPETGEHIETGGWRFEVVDLDGRRIDKVLASRVVRRPRRGALA"
     gene            complement(15415..16344)
                     /locus_tag="VF226_16430"
     CDS             complement(15415..16344)
                     /locus_tag="VF226_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7075626.1"
                     /translation="MVFGLASQAGAAFPERPITLIVPWAAGGGTDAVARYIGAVLQEE
                     LGQPVNVVNRTGGNGVVGHSAVATAEPDGYTIGLITLEINMMHWMGLTDLTYASYTPL
                     ALMNADPAAIHVRADSEFKTLNELVEAIKKNPGKFKSSGTAQGGSWHLALAGMLHSLG
                     ADPNSTQWVPSTGAATAMTDLAAGGVDLVACSLPEARALVEAGRVRSLAVMAEERLAV
                     FPDVPTLKEATGSTWTKGVWRGMVGPKGLPPEIAKRYEELFKKIYDSEGFKEFMAKRG
                     FETVWANANDFAEFMKRDDAEIGQMMKLMGLAK"
     gene            complement(16578..17489)
                     /locus_tag="VF226_16435"
     CDS             complement(16578..17489)
                     /locus_tag="VF226_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7075627.1"
                     /translation="MEAFVRVVNAGSFSAAARAMKLSPSAVSKLMSRIEGQLGVRLID
                     RSTRQFRLTPEGELYYKRCVKIVADIEEAEHAVTQHLIQPRGRLRVNSTIGIGIHRIL
                     PLMPEFLSCFPHIELDLSLSDKVVDLVEEQVEVAIRVGPLQDSSLVARKICESRRVVV
                     ASPDYLARNGTPRHPKDLLSHNCLTYNLRPSLNEWPFMIDGNVRPIAVKGSFCGDNGE
                     TLRHMAVAGGGIARLGWFQVGEDVRQGRLVPLLEGFHAGELQGVYAVFFGARHTSARV
                     RCFVDFLVDKLGRSRPWLAYEYASAAA"
     gene            17665..18573
                     /locus_tag="VF226_16440"
     CDS             17665..18573
                     /locus_tag="VF226_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7075628.1"
                     /translation="MKIFVTGATGYIGGSVATRLLADGHHVIGLARSEEAATSLKQRG
                     IEPVIGTLNDYGLLQRVARTADAVINAAQADNPFVVLAILPQLNGTGKVFIQTSGSSV
                     ISTYDQGEYVDRVFHEDTPFTPMPEKATRAAIDEQVLRAALDGVRSVVIRPSLIYGRG
                     LGVNSCSVQIPKLIEQAQSGVARHIGRGLNVWSNVHISDVVELYALALEHAPAGSLFY
                     AENGEESMKTVVEAISRMLGLGGRTQEWPIEEAAAALGPGAHLSFGSNSRVRAVKARK
                     VLGWAPKGPPLLHEIEQGAYKELYGR"
     gene            18614..18907
                     /locus_tag="VF226_16445"
     CDS             18614..18907
                     /locus_tag="VF226_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX7075629.1"
                     /translation="MPAYVIVDLQELIDADKLNAYRPHGAAAVKKYGGRYIARGGETA
                     SLEGGWTSQRITILEFDSLDQARAWYDSPEYAAARKIREGASRTRILAVEGVA"
     gene            complement(18953..20398)
                     /locus_tag="VF226_16450"
     CDS             complement(18953..20398)
                     /locus_tag="VF226_16450"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEX7075630.1"
                     /translation="MSGLYPVILSGGSGTRLWPLSRAMYPKQFIRFNAGDTSLLGATL
                     KRLRPEAGFRPPTILCNADHRFLVKENLDACNAGEGSIILEPVARNTAPAIAVGALSV
                     ARQDPAGTIVVMPSDHVIADEAGFLEAVQLAGKVAASGRLVMFGIKPTSAHTGYGYIR
                     RGKALEDFDNTAFRVEAFAEKPDAATAQLYVDDGHYFWNSGIFVLKVRTFLDELQRLA
                     PEILEFAQLAFDRATMDIGFLRLDGQPFAQCPNISVDYAVMEKTSVAAMVPVDIGWND
                     VGSWSSLWEIAPRDDQGNYVSHGEAVLESSSGCYVHSEKALVSTLGVENLVIVDTPDA
                     LLVADKSRAQDVSKIVARLKLGNRKEHEQHLRNYRPWGYFETLSLGPRFQVKLLHVKP
                     GGKLSMQMHHHRSEHWVVVHGTAKVTIGEREKLVRENESVYIVATQWHRLENPGKVPL
                     ELIEVQIGSYLGEDDIIRADDVYHRAPGETK"
     gene            complement(20565..22583)
                     /locus_tag="VF226_16455"
     CDS             complement(20565..22583)
                     /locus_tag="VF226_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEX7075631.1"
                     /translation="MQSSTADRNRDIWMKDPSIGRIVSVNGSKAVVLLDSAQDPTRRG
                     PEHRPEMGTLLVVEQTNTLVLAIVSALSVPVPAQREGDTELWIAELGLVGELWKDASG
                     NPASFNRGVTIYPALGDRVRVASRVELEMAFCGDRETAVRVGSLRQDPGIAAMVKVDD
                     LLGKHFAILGTTGTGKSCSTALILRAILRKNPAAHIVLLDPHNEYATAFTEWAEVLSP
                     RNLQLPFWLLTFEEAIEVLIGDPEGRKAEVEILQELIPIAKARYAMGRAKEIQALRRG
                     VLDPGRFTVDTPVPYRISDLTALIDERMGKLENRRDLHPYRQIKTRIDTISQDARYAF
                     MFGSLTVYDGMAQILGQIFRVPVNNKPITILELTGVPTEVVNVVVSVLCRMTFDFALW
                     SEGQVPVTLVCEEAHRYVPANPNVGFEPCKRAIAKIAKEGRKYGASLCIVTQRPAEID
                     PTILSQCNTVFALRMSNDRDQQIVTSAVSDTGSGLLEFLPALGQREAIAFGDGVSLPV
                     RIKFDELPRSALPRSSTARFSEKWQKSVGDEGFLDMIVERWRSCGASADHVSASGEEA
                     HELEPAAVLEEHPAPGSPAANGHVGRTREIPDPAMPRADLTQTGSAGPAPLGSLRRDS
                     SRPEAMAARTHQHQIRTGATSPATDASQQAADALRSLRERLMRSNSAR"
     gene            complement(22603..23481)
                     /locus_tag="VF226_16460"
     CDS             complement(22603..23481)
                     /locus_tag="VF226_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7075632.1"
                     /translation="MLIDGIEASKSNTLLSQYSAMLSTAVLRQRALGAERAARIETEL
                     ANRIKSEFIANMSHELRTPLNTIIGFSKLLSEHLQRRLKDEDIVDYASLIRDAAGHLL
                     SIINDILDISKIQSGKYTLDAQELSLGEVLQAMIPGLQLAAQEAGVQLECRIDPSLQP
                     IRGDAAKIRRIISNLLSNAIKFTREGGLVTVEAVRLPDGGALLIVRDTGVGMSEDEIR
                     IALMPFGQVDGTRSRWREGTGLGLPIARALVELHGGRLEIRSVKSKGTEVAVRLPSKH
                     QVSIAEAREAVLGGRM"
     gene            23596..24237
                     /locus_tag="VF226_16465"
     CDS             23596..24237
                     /locus_tag="VF226_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7075633.1"
                     /translation="MSFLPDIPTLLAYTAACAVLFLTPGPDMSLFLAKTVSGGRRAGT
                     AAMLGANLGCLVHTLLAAIGLSALLAASATAFALVKLVGAAYLLWLAIDAVRSGSALN
                     LKAQRSAREPFVRTLLLGAGVNLTNPKVVLFFVTFLPQFVRAGDPDAAAKLVFLGVYY
                     VLFSLPFAALMILGAERLLAFLKGRRGVLRAIDWTFAGVFGFFAVHILATQTR"
     gene            24328..25029
                     /gene="pcaH"
                     /locus_tag="VF226_16470"
     CDS             24328..25029
                     /gene="pcaH"
                     /locus_tag="VF226_16470"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX7075634.1"
                     /translation="MTLTYKPRDWSSQPGYIDPGYKSTALRGPTKPLIPIKQSLSELT
                     GPVYGHDSLGPLDNDLTRNGRRTGEPLGERIIVTGRVLDEDGRPVPHTLVEVWQANAA
                     GRYSHKVDQHDAPLDPNFFGGGRCVTDAEGRYTFMTIKPGAYPWGNHHNAWRPNHIHF
                     SLFGPSFATRLVTQMYFPGDPLLDLDPIYQSVPAGARERLVSRFSIDVTKPGFALGYE
                     FDIVLRGRNETPMED"
BASE COUNT         4391 a         8133 c         7888 g         4668 t
ORIGIN      
        1 gtgcccttct tgtgggcggc tttggcgatc ttggcggcgt tgtcgtagcc gatcttcggc
       61 gccaaggccg tcaccagcat gagcgagcgc tcgagcgctg ccttgatgtt gtcctcccgc
      121 ggctggatgc cgacgacgca attgtccgta aagcttcgcg ccgaatcggc cagcaggcgc
      181 accgattgca ggaagttgta ggccatcacc ggattgaaca cgttgagctg gaaatggccc
      241 tggctgccgg cgacggtcat ggtcgtatga ttcccgaaca cctgggcgca taccatggtc
      301 agcgcctcgc actgggtcgg gttcaccttg cccggcatga tcgaggagcc gggctcgttc
      361 tccggcagcg ccagctcgcc aaggccggag cgcgggccag agcccaggaa gcggatgtcg
      421 ttggcgatct tgaacagcga caccgcaacg gtgttgatgg ccccgtgcgt catgaccatc
      481 gcgtcgtggg cggccagcgc ctcgaacttg ttcggcgccg aggtgaacgg cagcccggtt
      541 atcgcggcaa tccgctcggc gaccttctcg gcgaagccct tgggcgcgtt gaggccggtg
      601 ccgacagccg tgccgccctg ggccagctcc atcagcatcg gcatggtcat ctcgatgcgc
      661 ttgatgccat tctcgacctg cttggcgtag cccgagaact cctggccgag cgtcagcggc
      721 gtcgcatcct gcgtgtgcgt gcggccgatc ttgatgatgt gcgcccacgc tttcgccttg
      781 gcgtcgagcg ccccgtgcag gtggtgcagc gccggcagca gctcgtgatg aacctgctcg
      841 gccgccgcga tatgcatggc cgtcggatag gtgtcgttcg acgactggct catgttgacg
      901 tgatcgttgg gatgcaccgg cttcttcgag cccttctccc cgccgagcat ctcgatcgcc
      961 cggttggaga tcacctcgtt ggcattcatg ttcgtttggg tgccggagcc cgtctgccag
     1021 acgacgagcg gaaaatgctc atccagcttg ccgtcgatga cctcctgcgc cgccttgacg
     1081 atcgcctcgc cgatcttcgg atcgagccgg ccgagcgcca tgttcgtttc cgcagcagca
     1141 cgcttgacga tgccgagtgc ccggatgatc ggcttcggtt gcttctccca gccgatcttg
     1201 aagttgccga ggctgcgctg cgactgagcc ccccagtatc tatcagccgg cacttcgatg
     1261 gggccgaagg tgtctgtctc gatgcgcacg tcagcggaag tcatctcaga agctcatttt
     1321 ttggtcgaaa ctgaagtcta ggcctcacta gcacagggca gggcccggct tccagcttca
     1381 cgccgctcgg tccgaacaag cccaagctcc cggctcgacc gggccgttcg attcgagggt
     1441 gcgcaagtcg cccgccatga agtctttgcg ttcataacga ctgatcagca gcaaagggga
     1501 agggtcagtg ctgcagcaat ggattgactt tcacatctat cgctttctcg cggccacgaa
     1561 ttggtcggaa ctgtccgtct tcttcatctt catactggcg atcgcgttcg gcctcgtcgt
     1621 gcacgccgca atcatgtggc tcgtgcgcaa attcttcccg gtgaagtccg acgaggtggg
     1681 cggccgcatc ctgaggcgca tttcgcgact cacacgtctg gctctcattc tgctggccat
     1741 caaaatcgcg ctgcccgcgg cgcgcttcga accggggatt cagcaggcgg cgctccgcgt
     1801 tctcgacctg ggtctcgtcg ccctcatcgg gctggcgctg atcgctgtca tcaatatcgg
     1861 gtgggacgtc gtcgagagcc acaatcggct cgacgtggag gacaacctgc gtgcacgcaa
     1921 gctgcgcaca cagatcggcg tgctgaggcg tacggccgtc atcatcatcg ccattctcac
     1981 gtgcggcggg atgatgatga cgtttccctc gctgcgcgag tacggcttga gcctgctggc
     2041 ctccgcgggc gctgcgggcc tcgtcgtcgg cctctccgcg cgtcccctgc tctcgaacct
     2101 gctcgccggt gtgcagatcg ccctcacgca gccgatccgc atcgacgata gcgtgatcgt
     2161 ggagggggag ttcgggcgcg tcgaggagat cacgaccaca tacgtcgtca ttcgcacctg
     2221 ggactggcgc cggatcgtga tgccgctttc aagcttcctc gagaagccgt tccagaactg
     2281 gacgctagag gattcttcgc tcatcggctc tgtgttctgg cacgtcgact attcggcgcc
     2341 gatcgaggag atgcggcgca agctgaacga tctgctgaag gaaagcgcct actgggaccg
     2401 tcgcgtcgct gcgctgcagg tcgtggaggc cgaggagcgg acgctcaaga tccgtgcttt
     2461 gatgagtgcc agaaacgcag cggccgcctg ggatctccgt tgccagatcc gagagcagat
     2521 gatcacatgg ttgcagaagg agcatcccaa cgctctgccg cgcgtcaggc tcgaagaacc
     2581 acatcgcgtc agggccaacg gcacctacgc cgagatcgct caggcggaat gatcagttct
     2641 gcccacaatt atccgatgtg agcctaagtt gctcacgttc ctgccatttt tctcgcgcct
     2701 gctcagttgt gcttgtgagg tcgagtcgcg ccgggcgggt gttggggcat ccttcggcgt
     2761 cagtggagcc ggatcgaacg ggttcggccc cgtggggcaa cgatcacgtg acctgcgtga
     2821 acctgggcag ccttcgcctg tccgttgtca accacgctcg aggatgcgcc ccgtgacccc
     2881 caagatcgtt gtgatgatca tcatgctggc gatgccgaat ggcgacagtg gtgtccatgt
     2941 gaagccgtat gccaccgcgg aaacctgcat cgaggccgca aacatcgagg cgaccgatcc
     3001 gttcgtccac tccgtggagt gcgccgagct cgaggacggc ctgctgacgc tgcgcttcga
     3061 ccgtccggat gtgccggaat caccgcccgt cacgaccggc gccctcgact cggcggagta
     3121 gcctgaaagg accgggccgc ggagccgccg tcaacagcta ttgaggtgcg cggggtgtga
     3181 ttggagttgt cggtggtgtc gtcggtacct cgacgccgtc gcctggcgtt gctgtcgtcg
     3241 tcggagcttc ttgccggccg aagatgtacc agagcaaccc gaggacgatc accaccgcca
     3301 gcagcaagcc gagcaggctg atgccattgt ccgaattgcc gttcctgagc atggcaggtc
     3361 tccgccgcgc ggcatgcgcc cgaacgaacg ccacccggcg cgtgcagttc caccgccgcc
     3421 tgcgcaaccg gcctacttga ccgccgagaa ggacgtcggc tgccagaact gattggagac
     3481 tttggcaatg atcggcccgt ccttctccga tgcagcgcgt gcctgctgcc attcggggtc
     3541 ggccatgaag gcgttccatt tctgctcgcg ttcggccatc gactcccagc ggagcagata
     3601 gttgagctgc tggttcgatt cgccgaccag tgtcgtccag aacccggcct gatgaatgcc
     3661 gtgacgctcc cagattttca gcgtcgtatt ttcaaagcgg ttcagcagtg ccggcaggcg
     3721 ccccggaacg cagtcgtaca cgcgcaactc atagatcatg ggcgaatccc ctcgattaag
     3781 aaccgtcgca tctggctgag cacaatttgg cggcgctgcc ttgcgaagct cggcccagtc
     3841 gaactcatgg cgttttcctc agtcctgccc cggcatcgga atccaattgt agagtgcgag
     3901 cccgccgtcg acgggtacag tcgcgccggt cacatagcca gagaagcgat ccgacaacag
     3961 cgctatcgcc atgccggcca tatcagccgc cgtaccgaat cgctgcaagg ggattttgcg
     4021 cttgggctgg aaggtctcgt ccatattggc agcgccaccg cccggcacgg cccctggcgc
     4081 cagcgcattg acccggatgc cggagggccc gaaggcgcgc gccagctccc taacgaccat
     4141 ggtcatgccg gccttggccg cggagtaatg ggcgagattg cgcggagcat aggcgtgcag
     4201 cgaggtgatg tagagcatgc gtccgcgcac gcccgcctcc tgcatccgct tgccgatctc
     4261 ccggcccagg aagaaaccgg accggacatt cgtgttgagc atggcatccc atgtcgcctc
     4321 cgacacgacg gtgggtatgt cgccctcgat tcggcgcggg caggccgcgt gcacgagcat
     4381 ctccggcaag cggccatcga ctgcctcgag aatggctttc catccatcgg gctgggaaag
     4441 gtcgaccgag atggtctcgg ccgagccgcc tgcatcgccg atctcgcgcg ccgcagcggc
     4501 attgcgctcc atgtcgatat ccacaaggac cacgtgagcg ccttcgcgcg ccagagccgt
     4561 cgcaataccg cggccgatgt tcgaggccga gcccgtcaca acggcgcgct cgcctgcgaa
     4621 cagtccctga gtgctcatgg tgcggctcct tcagatcacg gagcccgcat cggaacacag
     4681 aacgggctgt ttttgaagcg gcttggcggt gtgcggaacg gtatccgaaa atgcgtgccg
     4741 gccgggaggg cgcagcctac ttcttgcgga aggcgtccag gctgacgatc tgggcgccgc
     4801 cggagggcgg ccgctccgcc tcggtctcgt cggcggactg ctggacctgg gaatgagatt
     4861 tggacggaac ctcaggcgcc ggcttggaag atgcaggatg tgcagaggaa ggctctgtca
     4921 tgcccggctg atcggacggc ctgtccggcc gcgcctttcg cggactgcgc gtcggcttgc
     4981 gctcgactgc cggacgcggt gcaccgcgaa gcggcgccgg ttgcggccgc ggtgccggca
     5041 tggcctcttc ctcctcggca cccgtctcca ggcccgattg ggactgctcg agtgcctccg
     5101 ggcccaagtc cggcgcctcg aactgcaggc catagcgcac gctcggatcg aagaagatct
     5161 tcagcgccgc gaagggcacg acgagccgct ctggaatgcc gtcgaacgtc agcttcacct
     5221 cgaagcggtc ctcagtgacg ctgagatccc agaagcgatg ctggagcacg atcgtcatct
     5281 cttcagggta tttctcccga agacgcttgg agaggacgac gcccgggtgt tgtgtatcga
     5341 aggagatgta gaaatgatgg tcccctggca acccgctctt ggccgtacgg gccagcactg
     5401 cgcgcacgac gccccgcatc gcgtcctgcg ccaaagcttc atagtcgatc atgcgcccgc
     5461 tgctcatgag ggaccagggc catttccaga ttcgcgagcc ccgagactcc gggccgcagg
     5521 gaagtggagg gcttctgttg cccggtgccc tccgaaccgc gcctgaccag gctagtgatc
     5581 agggcttggg ttcaggcctt tcgcgccgcg ttacgcagct agagcagcct cagcatagtt
     5641 gtcattggca actattctgg ttggcccgat aacggcggaa ccatgccggg cgaaagctaa
     5701 gtcctttaca ccctcgtcga tcctatttcg cccccatcag agaccgccgc tttccaagct
     5761 gcggcctttg gtggaggcgc cgggtaccgc ccccgggtcc gataggctta ttacgaccgc
     5821 gtttatcgcc atagccggac gaatccagca tgcctaatat aggccgtccg tggcaaaaaa
     5881 aaagaactct tgaaagggta tgatcgcgct tgctcatctc cgccatacgc gagctgctga
     5941 cgggccagcc ggcctatcac gcccgctccc cctggcgccc ggtgcctgcc gtggcggcag
     6001 ctttaatcat ggtcgcgatt gccgccgggg tggccaccta tctcgttggc acgctgcaac
     6061 ttgcgccgcc aatcgcgatc cccatggacc tgactccggc ggacgagaac cggatctgga
     6121 ggatgacggt ctggctcctc agcatgcagg cctcgatcat cgcgctcgtt cttgtcgctg
     6181 ccggcatgtt cggcggccgg cgggccgagg tcctggcctt caatcgggac acgccgaaac
     6241 ggatctacgt ggcggccgtg atcgtcatgg tcatcgccca ggtcgcctac aacgtgatcg
     6301 tatttccatt cgcgcgcgac accatggtcg aggacgtgca gccgatgctg gagccgctgc
     6361 attccgatgc cttctggctg ttcgcgcttg cgatcgtcgt cggagctccg ctttcggagg
     6421 agctgctgtt ccgcggcttc ctgctgagcg cgctggccca gtcgcgcatc ggtttcctcg
     6481 gagccgcgct cgtgaccacg ctcggctgga cggctctgca cgccggctac tcgggcgtag
     6541 ggctcgtcga ggtcttcctt gcgggtctgc tgttctcttg gctcttgtgg agaacgggca
     6601 acctttgggt cccgctcgtc tgccatgggt tctacaacgg gatggtgctg ctcgccctcg
     6661 tcacgctgcc gctcacgtga ttgcgcgcac atccgggcca atgacgggac ggacgaaagc
     6721 tggatcggaa ccatggacca gtatctcaac ctgatgcgcc gggtgcgcac cgagggcatc
     6781 cgcaagaccg atcgcacggg cacgggcacg ctcagcgttt tcggccatca gatgcgcttc
     6841 gacctcgcgc gcggcttccc gctggtcacc accaagaagc tgcacctgaa gtcgatcatc
     6901 cacgagctga tctggttctt gaggggcgac acgaacatcg cctacctgaa ggccaacggc
     6961 gtctcgatct gggacgagtg ggcggacgaa catggcgacc tcggcccgat ttacggcaaa
     7021 cagtggcgct cctgggccgc cccgaatggt cgcaccatcg accagatcgc ggaagcggtg
     7081 gagactctga agaccaatcc ggacagccgc cgcatcatcg tcacggcctg gaacccggcc
     7141 gatatcccgg atatggcgct ggcgccctgc cattgcctct tccagttcta cgtggctgat
     7201 ggccggctct cttgccagct ttaccagcgc tcggcggacg tgttcctcgg cgttcccttc
     7261 aatatcgcca gctatgcctt gctgacgctg atgatggcgc aggtgacggg cctgcgtccc
     7321 ggcgagttca tccacacgtt cggcgacgcg cacctctacc tcaaccacct cgatcaggcc
     7381 gacttgcagc tcacgcgcac cccgcggccg ctgccgacga tgcggctgaa ccccgaggtg
     7441 aaagacattt tcggcttccg ttatgaggat ttcgagctga ctggctacaa tccccatccg
     7501 catatcaaag cgccggtggc cgtatgagcg atcgccccca gccagacgca gatccggtga
     7561 tcgcactcgt cgtcgccgcc gccgagaacg acgtcatcgg tcgcggcggc aggttgccat
     7621 ggcgtatccc ttccgacctg cggctcttcc gccgcctcac catgggcaag ccggtcgtca
     7681 tggggcgaaa gacgttccaa tcgatcggca agccgctcga cggccgcgat aacatcgtcg
     7741 tgagtcggga tccggccttt gatcctgccg gcgtgagcgt caagcggact ctggccgagg
     7801 ccatcgccga ggcccgcacg gccgccgcgg cccggggcca gaatgaaatc atggttatcg
     7861 ggggtgaggc ggtgttccgc gaggcgctcc ccctggctcg gcgcatctat ctcacgcgcg
     7921 tccatgggct acccgaaggc gatacatttc tgcccggaat cccgccaggc gagtgggacg
     7981 aagcttctcg cgaggatctt ccgcgcgacc caaccgacga gtttgcctgc acacttatcg
     8041 tcctcgagcg taagcctgag ccggtgcgtg acacagcctc cggttcttga agccccattg
     8101 cgccgcacca cattgcaccg agtacggttt gcgcgggcaa ggggtgtccc ctataacgac
     8161 ggatacaaag gcggatgatg agcgccccgc ggtgcgcggc tggcgccctt ttgcataccc
     8221 aggacacaag cagaaagcgg aaaagctatt caatgccttg gaacaatcag agtagtggtg
     8281 gtggcggctg gaaaagcggc ggtggcggcg gcccgtgggg cagcggcggc ggtggtggcc
     8341 cgtggggcca aggtcctggc ggcggcaatc agcccgatct cgaggagatc ctgaagcgcg
     8401 gccaagacaa ggtgaagcag gtgatgcatg gctccggcgt gccgggcccg ctcatcctcc
     8461 tgctgtcggc cgtggccctc gctgtcgtcg gtttctacgc atttacattc accgtacgcc
     8521 ctgacgagct cggcgtcgtg cttcgcttcg gccagttcca tcggcaggag ccgccgggcc
     8581 tgcatttcag gctgccttac ccgatcgaag aggtccgcct acccaaggtg accgtcgcga
     8641 accgtaccga aatcggcttc cgcggcccta cggacaaccg cgccagatcg atccagtccc
     8701 aggaggttct cgacgagagc ctgatgctga ccggcgacga gaacatcgtc gacatcggct
     8761 tcgtggtgct gtggaacatc aaggatgctg cggcctacct cttcaacatc gagaacccgg
     8821 agaagacggt caaagaggtc gccgagagcg ccatgcgcga ggtcgtcggc aagagccggg
     8881 tgcaggacgt gctgacggag aagcgctcgc agattgagca ggaagtgctt gatcttgtcc
     8941 agcgcaccct ggattcctac ggggccggca tcaacatcac gcagctgcag atgcagcggg
     9001 ccgaggcacc ttcgcaggtc atcgatgcgt tccgcgatgt tgccgcggcc gcgatcgacc
     9061 agcggcgaat cgagaaccaa gcctacgcct atgcgaataa ggtcattccc gaggcccgcg
     9121 gtgaggtgca gcgcatcctg caggaggccc aggcctacaa ggagcgggtg gttgcagagg
     9181 cgcaaggtga ggcagaccgc ttcctcaacg tgctcgagga gtaccgcaag gcgcccgagc
     9241 tgatgcgcca gcggctctac ctcgagacga tggagcgcgt gctgtcatcc accgacaagg
     9301 tcatcatcga tagcaatggc ggccagagcg tcgtgccata ccttccgctc gaccaattgc
     9361 agcccagaag acaacaacag cagcagtgag ggctcggatc atgcgcacaa cgttgatcac
     9421 aattctcgtc gcgctcggcc tggctgcggt cggtgcatac ctttcgatgt tcatcgtgaa
     9481 gcagcaccag caggcgctcg tgctggagtt cggcaagcca aagcggatca tcgacacggc
     9541 ggggctccat tggaagattc ctgtcgtgga aacggtcgag tacttcgaca agcgcattct
     9601 ggatctcgac acctctccgc aggagctttt ctcgtcggac cagaacaagc tgatcgtcga
     9661 cagcttcgcc cggtatcgca tcgtcgaccc gctgcgctac ttccagaccg tgcgcaacga
     9721 gctcggcgtg cgctcgcgcg tgggtccgat tctggacagc tcactgcggc gcgtcctcgg
     9781 ctcggcctcc tttgctgacg ccgtgcgtga ccggcgtgct cagctcatga acctgatcca
     9841 gaagcaggtg aatgccgagg ccaggaactt cggtattgag atcgtcgacg tgcgcaccaa
     9901 gcgcgtggac ctgccgccgc agaactcggc ctcgatctac gagcgcatga agacggaccg
     9961 gcagcgtgag gctgccgaat accgcgctcg cggtgaggag gcggcgcggc gcatccgggc
    10021 cgaagccgac cggcaggtcg ctgtcatgaa ggcggaagcc gagcgcgacg cgcggcgcct
    10081 gaaaggtgag ggcgacgccg agcgggccag gatttccaac gaggccttca agcaggatcc
    10141 ggagttctac cagttcctgc gctccatgga ggcttatgag acggccctga agcgcggcga
    10201 cacacgcctc gtgatctcac cggaatcgcc gacattccgc gacttcttcc gattcttctc
    10261 ggagtcggtc acaccggcga ccaaagttcc gaacgtcacc ggaacgaccg cgccgaacat
    10321 cgcgttgcca caacgatgag cgatctggct gtggccctcg gtctcgtgct ggtgatcgag
    10381 ggccttctct gggctgccgc tcctgatttg gcggttcgcc tactcaatgc tgcggcggaa
    10441 gcgccgccgc aggcgttgag aacagcaggt ctgacggccg ccgcggtcgg cgtattcatc
    10501 gtctggcttg tgcgcggctg aacgcccggc tctgtgtgtt gacaggctgg gggcccggat
    10561 cgaacgatat ggtggcgccg tcgcgcaaac gcagcgaagc ggcggatcgt gtgcgcatgc
    10621 aggtcgaaag gatacgtgtg atgacggccc tggcgcaggt gagagatgtg gggatcgcgc
    10681 gcactctcgc ggggttgatc ttttcgctcg cgttggtgac ttttgcgggc agctccgaag
    10741 cccgcgggcc ccaatcggtt gcggacgtcg ccgaagagct gatcgatgcc gtcgtcaata
    10801 tttccactac ccagaccttg aagactcccg agggcacgcc gctgccgcgt gtgcccgacg
    10861 gctcgccgtt cgaggatctg ttcgaggact ttttcaacaa gaagggcacc aatccccatc
    10921 gcgaccgccg cgtgtcctcg ctcggctccg gctttgttat cgatgggaaa gaggggctga
    10981 tcgtcaccaa cacgcacgtg atcgagggcg cggacgagat cgtcgtcaac ttcgccgacg
    11041 gctcgcgcct caaggtcgac aaggtcgtcg gcaaggacgg caagacggac ctggcactcc
    11101 tcaaggtcac gccgaaaaag ccgctcaagg aagtgaaatt cggcgcgtcc gaggcgatcc
    11161 gggtgggcga ttgggtaatg gcgatcggca atccgttcgg gctcggcggc tccgtgacgg
    11221 tggggatcat ctcggccaag gcccgcgaca tcaattcggg gccatacgac gactatctcc
    11281 agacggatgc cgccatcaac aaggggaact cgggcggacc tctgttcaac atggccggcg
    11341 aggtcatcgg catcaacaca gccatcattt ccccgaccgg cggctcgatc ggcatcggct
    11401 tcgccgttcc gtcggacatt gccatgccgg tgatcgaaca gctgcgcctt tatggtgaga
    11461 cccgccgcgg ctggctcggc gttcgcattc agaccgtcag cgaggacgtc gccgagtcgc
    11521 tgggcgtgcc accgaacacc ggcgctctgg tggccggcat ttcacccgac agccccgcag
    11581 cgaaggcagg catcgaggcg ggtgacgtca tcaccaagtt cgatggcaaa gaggtgacga
    11641 gcatgcgggg gctgccgcgc atcgtggcgc agacgcccat cggcaagacc gtaccggtcg
    11701 aggtcatgcg taagggcgaa aagaagacac tcgaggtcgt ggtcgggcgg ctcagcgagg
    11761 acgatgatgc tgctgccgaa gagcctgcgc ctccgacgac gcgcggtgaa actccagcca
    11821 acccaggcca gggacttatc gggttgaagg tcaccccgtt gacggacgag ctccgtcgca
    11881 agcacggcat tgaaaagaag atcaagggcc tgctcgtcac cgaggtcgac caggacagcc
    11941 ccgccgccga gaaaggcgtc aaggccggag acatcatcgt cgaggcggcc caggagcaag
    12001 ttgcgacggc cgacgatctg acgaagagcg tcgacaaggt gcgcaagtcg gggcggaaag
    12061 ccgtgttgct gcggctcgag gacggcaagg gcgacatgcg cttcgttgcc gtcccaatcg
    12121 actgacgggg cttcgtccaa attctgcgca tccgagcggg tatagagact gactgccgca
    12181 gcggcgccgt gcctgcgctc gtcacgcggg gcgacatgcc actggcgctg cattgcggcc
    12241 ggcgctaaga catgcgaccc tttccaaccc gctggagaag acaagcttgt tgtccgaaaa
    12301 tcgcagcctt ttgagcacga ttgcggattc gatcccgccc gttcatcgcg acggccacaa
    12361 gttcgtggcg atcggcctgg gtataacgtt tctgttcttc ttcctctggc cgccgttggg
    12421 atgggtattc gccgtcgtca cggcctgggt gctctacttc ttccgcgatc ccgaccgcgt
    12481 cacgccgctg cgggaagggc tcgtcatcgc ccccgcagac ggccggatca gtgcgatcga
    12541 gcgggtacgc ccgccgcagg agctggggct cggcgaggag cttcggaccc ggatatcgat
    12601 tttcctgtcc gtgttcgatg tgcacgtgaa ccggtcgccg gtcgctggac gtatcacgcg
    12661 gtccgtctat accccggggg cctttttcaa tgcggagctc gataaggcca gcgaggaaaa
    12721 cgagcgccgt ggcctcgtca tcatgatgcc ttcgggcaac gaaatcggcg tggtgcagat
    12781 cgcggggctc gtggcaaggc gcatcgtgac cttcgtcggc gagggcgata cggttggtgt
    12841 cggccagcgg ttcggcctta ttcgtttcgg ctcacgcgtg gacgtctatc tgccgcctgg
    12901 cgcccgacct ctcgtggccg ttggccagcg gaccattgca ggcgagacgg tgcttgccga
    12961 cctcaagtcg gatgagccgg agcgcgaggc gcgccggagt tgagggctgg atggaacacg
    13021 cagaattgct gaccggccgc ggacgggagc agacccgatg gaccctctgt tgacgaggct
    13081 tgaagaagag acccgccggc gcaggcgccg cgcaatcttc aagtacaggc aagtcccggt
    13141 ccgcctgctc attccgaatt tcttcacgct gctcggactg tgcgcggggc tgaccgccat
    13201 ccgcatggcc atcgaaagcc gttatgagct cgctctcgcc gccatcgttt tcgcggctct
    13261 gctcgacggc gtcgatggcc gcgtcgcccg catgctgaaa gcctcgtcgc ggttcggcgc
    13321 cgagttcgat agcctagccg atttcgtcaa cttcggcgtc gctcctgcca tcgtgctctt
    13381 tacctggggc gcgtgggaaa cacccgctat gcggggtctg gcgtggatcg tggtcctgct
    13441 gctggcggtc gcctgcggcc tcaggcttgc gcgtttcaac accgcgctcg atgtggaacg
    13501 gccgaagtgg cagtccaatt acttttccgg agtgcccgcg ccggccggtg ccatcatcgc
    13561 gctgctgccg gtctacctgg atcagctcgg gcttctgcag atgcgcatcg gctggcccat
    13621 ctacttcgtg ctcgcctacg ctctgctcat tgccgtcctc atggtcagca cgatcccgac
    13681 cttctccggc aagctccttg gcgagcgcat ttctcgcgag tgggtgatgc ccatattcgt
    13741 gctcctgggt gggctggtgg cgctgctcgt gacctacccg ttcgccacgc tcagcatcgg
    13801 cacgatactc tatctggcaa tgatcccgtt cagcatccgc cgttatcgca gccagatgct
    13861 ggatatggag gcagaggaaa gacggcggcc cgaggacaac atcccgaaac tggtggtcga
    13921 caagccctct caccccgacc ggatgccacg ttgagccgcg gagcgggcca cggggtcgtg
    13981 cgttgaccgc tcgagcggtg ctacctagta tttcaccgac ctgcaagatg gagtggcttg
    14041 gacgcgaaca cgtccgccgc ggatgacgtg atcgaaatcc tcatcgtcct ggctcttttt
    14101 ctcctcaacg gcctcttcgc actttcggag ctggcggtcg tctcctcccg gcattcgaga
    14161 ctgaaggcca tggcagccgc aggccgccgc ggagccaacc gcgccctcga gcttgcgacc
    14221 aaccccagcc gctttctctc gaccgtccag atcggcatca cgctcgtcgg catcgtcagc
    14281 ggcgcttact cgggcgaggc cttcgggatg cgcgctgccc agtacctgag cagcgtcgga
    14341 ctgccaaccg gtgcagccat cacgatcggc tatggcctgg tgatcgtgat cgtgacctac
    14401 ttctcggtca tcatcggcga gctcgtgccg aagaccctgg cgctgcgcaa cgcggaaggc
    14461 cttgcctgcg cggtcgcacc gatcatgatc attgtcgaca ggatggcggc gccggccgtt
    14521 tggctgctcg acagctcgac ccgtctcgtc tttcgcgtgc tcggcctgcc gaccgaacgc
    14581 gagatccgcg tcaccgacga ggaaatcaag gccttgatcg cggaagccga acgcgccggc
    14641 gttctcgaga ccggcgagcg cgagatgctc tccggcgtca tgcggctggc cgaccggagc
    14701 gtcgtcggtc tgatgacgcc gcggacggag gtcgactgga tcgacatcac cggctcgcat
    14761 gaggagatca aggagcggat catcagcacc ccgcattcac gcttgcccgt cggcgagggc
    14821 tcggtcgacc agatggtggg cgtcatccag actcgtgagc tgcttgccac gctgctgtcc
    14881 ggaaaatcgt tcgacgtgcg ctcgctcatc cgcaaggcgc cgatcatacc cgagaccacc
    14941 gacgcgctgg atgcgcttgc catcttgcgt gatgcagagg tcccgatggc gctcattcac
    15001 gacgagtacg gcaatttcca gggactcgtg acaccggctg acgttctgga agccattgcc
    15061 ggggcgttcc ggtccgatac ggcaggggaa gtgtacgccg tcgagcgtga ggatggctcg
    15121 tggctgcttt cgggcgcaat gccagccgat gaaatggccg acaagctcgc gatcaaactt
    15181 cccgaaaaac gggcctacga gacagtggcc ggcttcgtgc tcgcccacct gcagcatctg
    15241 cccgagaccg gcgagcacat cgagaccggt ggctggcgct tcgaggtcgt cgacctcgac
    15301 ggccgccgaa tcgacaaggt tctcgccagc cgcgtcgtcc ggcgcccccg gcgaggggcg
    15361 ctggcgtgac gggcgcggca atccaggtgc cgcgctcgcc ggaaatcgga tgaactactt
    15421 cgcaaggccc atcagcttca tcatctggcc gatctcggcg tcatcgcgct tcatgaattc
    15481 agcgaaatca ttcgcattgg cccacacggt ctcgaacccg cgcttggcca tgaactcctt
    15541 gaagccttcg ctgtcgtaga tcttcttgaa cagctcctca tagcgcttgg cgatctccgg
    15601 cggcagtccc ttcggtccca ccatgccgcg ccatacgccc ttggtccagg tcgacccggt
    15661 cgcctccttg agcgttggca cgtccgggaa cacagctaaa cgctcctccg ccatgacagc
    15721 caggctgcgg actcgcccgg cctccaccag cgcacgcgcc tccggcagcg agcatgcgac
    15781 gagatccacg ccccccgccg ccagatcggt catggctgtc gccgctccgg tgctcggaac
    15841 ccactgcgtg gaattggggt cggcgccgag tgagtggagc attccggcca gcgccaggtg
    15901 ccagctgcca ccctgggccg ttcctgacga tttgaacttg cctgggttct tcttgatcgc
    15961 ctcgacgagc tcgttcagcg tcttgaattc cgagtcggct cgcacgtgaa tggcggcagg
    16021 gtcggcgttc atcagcgcca gcggcgtgta ggacgcatac gtgaggtcgg tcagccccat
    16081 ccagtgcatc atgttgatct cgagcgtgat caacccgatc gtgtagccat ctggctccgc
    16141 cgtcgcgacg gcggagtggc caacgacacc gttgccaccc gtgcggttga cgacattcac
    16201 cggttgcccg agctcttcct gcaggacggc gccgatgtac cgggccaccg catcggtccc
    16261 gccacccgca gcccacggca cgatcaacgt gatcggccgt tcggggaatg cggcgccggc
    16321 ctggcttgcc aaaccgaata ccattgcggt cgcgcagatc gccgaccgca gccacttcca
    16381 tgaggtcatc aacattctcc agatttcctc cccaacactg tcaggcgcca gctggtcgcg
    16441 tgcagcatcg acccgctgcc ctgatcggga cagccagagg atatccggac acctgctgat
    16501 tggtcgtcat agaacttttg ccgccggttc atattcagcc ggccgaggtc cgcctcgagc
    16561 cgtgtgccgt tccggtgtca agcggcggcg gaggcgtact cgtaggccag ccagggccgc
    16621 gaccgcccca gcttgtcgac aaggaagtcg acaaagcatc tgacgcgcgc agaggtgtgc
    16681 cgcgccccaa agaacacggc gtagactccc tgcaattcgc cggcgtgaaa gccttccagc
    16741 agtgggacca gccggccctg acgcacatcc tcaccgacct gaaaccagcc aaggcgcgcg
    16801 atgccccctc cagccaccgc catgtgccgg agcgtttcgc cgttgtcacc gcagaagctg
    16861 cccttgacgg cgatcgggcg gacgtttccg tcgatcatga aaggccattc gttcaggctg
    16921 ggccgcagat tgtacgtcaa gcagttatgg gaaaggaggt ccttcggatg ccggggagtc
    16981 ccgttccttg caaggtagtc gggggaggcg acgaccaccc gccggctctc gcagatcttg
    17041 cgcgccacca gcgagctgtc ctgcagcgga cccacgcgaa tggcgacctc cacctgctcc
    17101 tcgacgaggt cgaccacctt gtcgctcagc gacagatcca gctcgatgtg gggaaagcag
    17161 ctcaagaatt cgggcatcag cggcaggatc cgatgaatgc cgatgccgat cgtcgagttg
    17221 accctgagcc tccctctcgg ctgaatgaga tgttgcgtga ccgcgtgctc cgcttcctcg
    17281 atatcggcga cgatcttgac gcagcgcttg taatagagct cgccttcggg agtcagcctg
    17341 aactgccgtg tcgagcggtc gatcaaccgc acgcccagtt gaccctcgat gcgcgacatg
    17401 agcttgctga cggcagatgg agacagcttc atcgcgcgcg ctgccgcaga aaagctgcct
    17461 gcgttgacga cacgtacgaa tgcctccatc cgaccgaccc ggtcgtgttc cctttccgtg
    17521 ttgcgcgctc ctccccggtg aattcttttc acggccccta ttcccatttt acctctggca
    17581 tcagctcccc accttcagca gactccagca cgaaggcgcg gagcggaaaa gccttacgcg
    17641 gatctgaagg aggacagccg atccatgaag attttcgtca caggcgcgac cggctacatc
    17701 ggcggatccg tcgccacgcg tctgctggct gatggacatc atgtcatcgg gcttgcgcgc
    17761 agcgaagagg cagcaaccag cctcaagcaa cgggggatcg aaccggtcat cggcacgctc
    17821 aacgattatg gcctcctcca acgagtggcc cggacggcag atgctgtcat caatgcagct
    17881 caagcggaca acccttttgt cgtcctcgct attctgccgc agctcaacgg gacgggaaag
    17941 gttttcattc agacgagcgg ctcgagcgtc atcagcacct acgaccaggg tgagtatgtc
    18001 gatcgcgtct tccacgaaga tacacctttc accccgatgc ccgagaaggc gacgcgggcg
    18061 gcgatcgacg agcaggttct ccgtgcggcg ctcgacgggg tgcgttctgt cgtcatccgt
    18121 ccgtcgctga tctacgggcg cggcctgggg gtcaactcgt gcagcgtcca gatccccaag
    18181 ctcatcgagc aggcacaatc gggtgtcgcc cggcacattg gacgcgggct gaacgtctgg
    18241 tccaacgtcc atatctcgga cgttgtcgaa ctctatgccc tggctctgga gcacgcgccg
    18301 gccggctcgc tcttctacgc cgagaacggc gaggaatcga tgaaaacggt cgtcgaggcc
    18361 atcagccgca tgctcggcct tggtgggcgc acgcaagagt ggccgatcga agaagcggca
    18421 gccgccctcg ggccgggcgc gcacctttcg ttcggatcga acagccgggt gcgagcggtg
    18481 aaggcacgca aggtcctcgg ctgggcgccg aagggacctc cgcttctgca tgaaatcgag
    18541 cagggcgcct acaaggagct ttacggccgc tgaggctgtg ctgcgcaacg gccaagacta
    18601 cgaggaatga tgaatgccgg cttacgtcat cgtggatcta caggagctga tcgacgcgga
    18661 taagctgaac gcgtatcgcc cgcacggcgc ggcagctgtc aaaaaatatg gcgggcgtta
    18721 catcgcgcgt gggggcgaga ctgccagcct ggaaggcggc tggacgtctc aaaggatcac
    18781 catcctggaa ttcgactctc tggatcaggc ccgcgcctgg tacgactctc cggaatacgc
    18841 cgccgcgaga aaaatccgtg agggcgcctc gcgaactcgc attctggcag tggagggcgt
    18901 ggcgtagcac gcggatgatg atgggcgctt tgctggcaac cgaagcggcc gatcacttcg
    18961 tctcgcccgg cgcgcggtga tagacgtcat ccgcccgtat gatgtcgtcc tcgccgaggt
    19021 agctgccgat ctggacctcg atgagctcga gcggcacctt ccccgggttc tccaggcgat
    19081 gccattgcgt cgcgacgata tagacgctct cgttctcgcg cacgagcttt tcgcgctcac
    19141 cgatggtcac cttggccgtg ccgtggacga cgacccagtg ctccgagcga tgatgatgca
    19201 tttgcatgga gagcttgcca cccggcttga cgtgcagcag cttcacctgg aagcgcgggc
    19261 cgagcgacag ggtctcgaaa tacccccagg gtcggtagtt tcgtaaatgc tgctcatgct
    19321 ccttgcggtt gccgagcttc aagcgcgcga cgatcttgga gacatcctgg gcgcgagact
    19381 tgtcggcgac cagcagggcg tcgggcgtat cgacgatgac gaggttttcg acgccgagcg
    19441 tggacaccag cgccttctcg ctgtgaacgt agcagccgga tgagctctcc agcactgcct
    19501 cgccatggct cacgtaattg ccctggtcgt cgcggggcgc gatctcccat agcgacgacc
    19561 aggagccgac atcattccat ccgatgtcga ccggcaccat cgctgccacc gaggttttct
    19621 ccataacagc gtagtcgacc gaaatgttgg gacactgggc aaagggctgc ccgtcgagcc
    19681 tcaaaaagcc gatgtccatg gtggcccgat cgaatgcgag ctgcgcaaat tcgaggatct
    19741 cgggcgcaag ccgctgcagc tcatcgagaa atgtcctcac cttgaggacg aagatgccgc
    19801 tgttccagaa gtagtgtccg tcatcgacgt aaagctgcgc cgttgcggcg tcaggctttt
    19861 cggcgaaggc ctcgactctg aaagcagtgt tgtcgaagtc ctcgagggcc ttgccgcggc
    19921 ggatatagcc gtatcccgta tgagcgctgg tcggcttgat cccaaacatc acgagacggc
    19981 cgctggcagc aacctttccg gcgagctgaa cagcctcgag gaagcctgcc tcgtcggcaa
    20041 tgacgtggtc cgacggcatg acgactatgg tgccagcggg gtcctggcgg gcgacgctca
    20101 aagcaccgac ggcgatcgcc ggggccgtgt tgcgcgccac cggctccaga atgatgcttc
    20161 cctcaccggc attgcatgcg tccaggttct ccttgacaag aaaacggtgg tccgcgttgc
    20221 acaaaatagt cggcggccta aacccggctt cgggccgcag gcgtttcaag gtggcgccga
    20281 gcagactggt atccccagcg ttgaagcgaa tgaattgctt cgggtacatg gcccgggaga
    20341 gcggccacaa acgcgttccc gagcctccag acaaaataac gggataaagt ccgctcatgc
    20401 ccagtcctcc agcgccagcg gatccaccgc gcccgtagga ccaagtctag cggccggatg
    20461 ctagcagaac cagcgccatc cacgtgtaag ttgtgggata gggataacgc gacggtccga
    20521 cctgttctgc ctcgcaaacc ctgcacgagc ctcgtctcag ccgatcagcg ggctgaattg
    20581 gagcgcatga gccgttcacg cagcgagcgg agcgcatcgg cggcctgctg tgaggcatcc
    20641 gtggccggac tggttgcgcc agtgcggatc tggtgctgat gcgtgcgcgc cgccatcgcc
    20701 tcgggccgcg acgaatcccg gcgcaggctt ccgagcggag ccggcccggc gctcccggtc
    20761 tgcgtcaagt ctgcccttgg catcgccgga tcgggaattt cgcgcgtccg gccgacgtgt
    20821 ccgttcgcgg ccggcgagcc cggcgccggg tgctcttcca gcacggcggc cggttccagt
    20881 tcgtgggctt cctcaccgct tgcactgacg tggtccgcac tggcgccgca cgaccgccaa
    20941 cgctcgacga tcatgtcgag gaaaccttcg tcgcccacgg acttctgcca tttctcgctg
    21001 aaccgcgccg tggagctgcg cggcagggcc gagcgtggca gctcgtcgaa cttgatacgc
    21061 acgggcaggc tgacgccgtc gccaaacgca atcgcctccc gctgaccgag cgccggcagg
    21121 aactccaaca ggccagagcc tgtgtcggac accgccgatg tgacgatctg ctggtcgcga
    21181 tcgttggaca tccgcagagc gaaaacggtg ttgcactggg acaggatcgt cgggtcaatc
    21241 tccgccgggc gctgtgtgac gatgcacagc gaagcgccgt acttgcgtcc ttccttggca
    21301 atctttgcga tggcccgctt gcagggctcg aagccgacgt tgggattggc cggaacgtag
    21361 cggtgcgcct cctcgcacac gagcgtcacc ggaacctgcc cctcgctcca cagtgcgaaa
    21421 tcgaatgtca tccggcagag caccgacacg acaacgttaa cgacttccgt cggcacgccg
    21481 gtcagctcga gaatggtaat cggcttgttg ttgacaggga cacggaagat ctgtcccagg
    21541 atctgcgcca tgccgtcata aacggtgagg gaaccgaaca tgaaggcata ccgcgcatcc
    21601 tggctgatcg tgtcgatgcg cgtcttgatc tggcggtaag gatggagatc gcgcctgttc
    21661 tcgagcttgc ccatgcgctc gtcgatcagc gcggtcagat cggagatgcg gtagggaacg
    21721 ggcgtatcca ctgtaaaccg gcctggatcc aaaacgcccc gtcgcagcgc ctggatctcc
    21781 ttggcgcgcc ccatggcata gcgcgccttg gcgatcggga tgagctcctg caggatttcg
    21841 acctccgcct tacgtccctc cgggtcgcca atcaggacct cgatcgcctc ctcgaacgtc
    21901 agcagccaga acggcagctg gaggtttcgc ggcgagagca cctcggccca ctcggtgaag
    21961 gccgttgcgt actcgttgtg cggatcgaga agaacgatat gggcggcagg gttctttcgc
    22021 aggatggcac gcaggatcag cgccgtcgag caggacttgc cggtgcccgt cgtgcccagg
    22081 atggcgaagt gcttgcccag gaggtcatcg accttgacca tggccgcgat gccgggatcc
    22141 tggcgcagcg agccgacgcg gacagcagtt tcacggtctc cgcagaacgc catctccagc
    22201 tcgacgcggg aggcgacgcg gacgcgatcg cccagcgccg ggtagatcgt aacgcccctg
    22261 ttgaacgaag ccgggttgcc gctcgcatcc ttccacagct cgccgacgag gccgagctcg
    22321 gcaatccaca attcggtatc cccttcccgc tgcgcgggca caggcacgct cagtgccgag
    22381 acgatggcga ggaccaatgt gttcgtctgt tccacaacga gcagcgtgcc catttccggg
    22441 cgatgctcgg ggccgcggcg ggtcgggtcc tgtgccgaat ccagcagcac aaccgccttc
    22501 gagccgttga ccgaaacgat gcgtccgatc gacgggtcct tcatccagat gtcccggttc
    22561 cggtcggctg tactcgactg catggcgccc cctcgatccc tgctacattc ttccgccgag
    22621 cacggcctct cgcgcttcgg cgatcgaaac ctggtgcttg gacggcagtc tgaccgctac
    22681 ctcggtgccc ttcgacttca cgctgcggat ttccagccgg cctccgtgca gctccacgag
    22741 cgcccgggcg atcggcaatc cgagcccggt gccttcccgc cagcgcgacc gggtcccatc
    22801 aacctggccg aagggcatga gcgcaatgcg gatttcgtcc tcgctcattc ccacgcccgt
    22861 atcgcggacg atgagcaggg ctccgccatc gggcagacgg acagcctcga ccgtgacgag
    22921 gccgccttcc cgtgtgaact tgatcgcgtt gctgagaaga ttggagatga tccgccggat
    22981 cttggcggca tcgccgcgga tcggctgcag cgaggggtcg atgcggcact caagctgcac
    23041 gccggcctcc tgcgcggcca gctgcagtcc cggtatcatg gcctgcagca cctcacccag
    23101 gctcagttct tgagcatcca gcgtgtactt gccgctctgg atcttcgaga tgtcgaggat
    23161 gtcgttgatg atcgacagca gatgtccggc cgcatcacgg atgaggctgg cgtaatcgac
    23221 gatgtcctcg tccttgagcc ggcgctggag atgctcggaa agcagcttgg aaaagccgat
    23281 gatcgtattg agcggtgtcc gcagctcatg gctcatgttg gcgatgaact cggacttgat
    23341 ccggtttgcg agctccgtct cgatacgcgc cgcgcgctca gcgcccaagg cccgctgcct
    23401 caagacggcc gtactgagca ttgccgaata ctgactcagc agggtatttg acttgctcgc
    23461 ctcgatcccg tcgatcagca tgaacggcca caaacgcttt tacgctgcaa tgatcataag
    23521 gtagacggta ctttttaaag ccggggttaa ttgcgggcgt gcggccggca gcaaccgttg
    23581 catagagcac atcggatgtc gttcctgccc gatattccca cgctcctcgc ttacactgcc
    23641 gcctgcgctg tcctgttcct gacgcccgga ccggacatga gcctgttcct cgccaagacg
    23701 gtctcgggcg ggcggcgagc cgggacggca gccatgcttg gcgcgaacct gggttgcctc
    23761 gtccacacat tgctggcggc gatcggcctc tctgcgctgc tcgccgcctc ggcgaccgcc
    23821 ttcgcgctcg tcaagctggt cggcgcggcc tacctgctgt ggcttgccat cgatgccgtc
    23881 cgctccggct cggctctgaa cctgaaggcg caaaggtccg ctcgcgagcc gttcgtcagg
    23941 acgctgctgc tcggcgccgg ggtcaatctg acgaacccca aggtcgtcct gttcttcgtg
    24001 accttcctgc cccaattcgt gcgggcgggc gaccccgatg ccgcggcgaa gcttgttttc
    24061 cttggagtgt attacgtcct gttctctctg cccttcgccg ccctgatgat cctcggcgcc
    24121 gaacggctgc tcgcgttcct caagggacga cgcggtgtgc tgcgcgccat cgactggacc
    24181 ttcgccggcg tgttcggctt cttcgccgtg cacatcctcg cgacccaaac tcgctgattg
    24241 cggagcggcc ctgcgggatt agaattgacc cgacgcggag gggcgcggag gcagatcgac
    24301 ggcgcccgat acgaacggga ggagacagtg acgctcacct acaaaccgcg cgactggtcg
    24361 agccagcccg gctacatcga tccaggctac aagtcgacag ccctccgggg cccgacgaag
    24421 ccgctgattc ccatcaagca gtccctctcc gagttgaccg gccccgtcta cggccacgac
    24481 agccttggcc cgctcgacaa cgatctcacc cgcaacggcc ggcgcaccgg cgaacccctc
    24541 ggtgagcgga tcatcgtcac cggccgcgtc ctcgacgagg atggccggcc ggtgccccac
    24601 acgctggtag aagtctggca ggccaatgcc gctggccgct acagtcacaa ggtcgaccag
    24661 cacgacgcac cgctcgaccc gaactttttc ggcggcggcc gctgcgtcac ggatgcagag
    24721 ggccgctaca ccttcatgac gatcaagccc ggcgcctacc cgtggggcaa ccaccacaac
    24781 gcctggcggc cgaaccacat ccacttctcg ctgttcggac caagctttgc tacgcggctc
    24841 gtcacgcaga tgtattttcc cggcgatccg ctgctcgatc tcgaccccat ctaccagagc
    24901 gtgcccgccg gcgcccgcga gcggctcgtc tcccgctttt ccatcgacgt gacgaagccc
    24961 ggtttcgcgc tcggctacga attcgacatc gtcctgcgcg ggcgcaacga gacgccgatg
    25021 gaggactgac gccgtggccc tcaaacagac gccgtcgcag acggtcggcc ccttcttcgc
//
LOCUS       DASVDU010000250         4220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_56916, whole genome shotgun sequence.
ACCESSION   DASVDU010000250 DASVDU010000000
VERSION     DASVDU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56916"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF226_16475"
     CDS             complement(<1..256)
                     /locus_tag="VF226_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018072625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HEX7075635.1"
                     /translation="MQPSSRQIAAGKAAVFALALLPFGWLATGALFFPDWLTANPGEF
                     IDRETGRWTLRFLLITLALTPLRLITGWNWLLRFRRMLGLF"
     gene            complement(256..1215)
                     /gene="msrP"
                     /locus_tag="VF226_16480"
     CDS             complement(256..1215)
                     /gene="msrP"
                     /locus_tag="VF226_16480"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEX7075636.1"
                     /translation="MIIKQPNDVPPSEITPKGIYEHRRRFMKIAAGASVLGATGALWR
                     GAAAQEKTLGLQKLENVAMSRFSTSEPPTAYKHVTSYNNYYEFGTDKDDPADYARRLR
                     IRPWTVAVDGEVAKPRTFGIEDLLKFPLEERVYRLRCVEAWSMVIPWIGFEFNRIAKL
                     VEPTSKAKFVEFVTALQPETMPGLRRPVLDWPYVEGLRIDEAMHPLTILAVGLYGEVL
                     PNQNGAPVRLVVPWKYGFKSGKSLVRIRFVEHQPKTAWEKAAPHEYGFYSNVNPNVDH
                     PRWSQKRERRIPDFFARRETVIFNGYGEQVASLYAGMDLKKLY"
     gene            complement(1803..2363)
                     /locus_tag="VF226_16485"
     CDS             complement(1803..2363)
                     /locus_tag="VF226_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075637.1"
                     /translation="MDEELNLVTEWLLSASSEIPAEYFQLPVAGAEEPQYRERVYCYE
                     LYHRWRNRWPDGFPFSLSGEVDKQGHRLVRGGWKPDFLVHVPGRMSNLLIVEVKPANA
                     EPARMADDLKKLTHFRRELRDQYGNPGNYRGAYLWIYGLRAAEWPGLRAEVLGSLHGD
                     PQFDRDLVSCIIHETAQARATPAPWG"
     gene            complement(2392..3039)
                     /locus_tag="VF226_16490"
     CDS             complement(2392..3039)
                     /locus_tag="VF226_16490"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7075638.1"
                     /translation="MKLYGYRNGRTFRALWALEEIGAQYEYVEIDLMRGEGREPKFLE
                     INPAGKVPVLDDDGAIITESAAICMHLAEKHPQSRLLPPAGTVERSQCYKWMSYVLTE
                     LDAPLWAIAKHRFALPRDRRVPAVIETSSWEFRTAVKILATDLGNRSFLVGDRLTVAD
                     ILAGHTLSWAKSARLALGSEQLEGYLASLLARDAAIRARERAQPPLVKVASQSDA"
     gene            complement(3044..4132)
                     /gene="mnmH"
                     /locus_tag="VF226_16495"
     CDS             complement(3044..4132)
                     /gene="mnmH"
                     /locus_tag="VF226_16495"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HEX7075639.1"
                     /translation="MASPSNHPDPRRSFPELLERALEGQYELVIDARSEREYAEDHLP
                     CAVNLPVVDNDEYAEVGTTHRTDKHRAYLVGVSYALKNMSRSIEELVARYPKDARMLV
                     YCFRGGKRSKLWFDALSTIGFRVERLPGGWKAYRAWVREQLAELPEKFRYHVLCGPTG
                     CGKTRLLNALAETGAQVLDLEGLASHRGSLIGDIPGISQPTQKWFDSLLVDKLRRFDP
                     SRPVWVESESKKIGAIQLPGELLQAMHAGMIFSVTAPMSERVKLWREDYGHFERDLDA
                     MMERLRHLRPLVGGEEFLLWERLARERRAPELFQRLMEAHYDPAYARSIGRNYPEIER
                     ASKIELTRLDQESLLQAARQLQKEPATA"
BASE COUNT          813 a         1340 c         1291 g          776 t
ORIGIN      
        1 cgaacagtcc cagcatgcgc cggaatctga gcagccagtt ccagccggtg atcaggcgca
       61 gcggcgtcaa ggcgagcgtg ataaggagga agcgcagtgt ccagcggccc gtttctcggt
      121 cgatgaactc gcccgggttc gcggtcagcc agtcggggaa gaagagcgcg cccgttgcga
      181 gccacccgaa cgggaggagc gccagcgcga acacggccgc cttcccggcc gcgatctgcc
      241 tcgaagaggg ttgcatcaat agagcttctt gagatccatg ccggcgtaga ggctcgccac
      301 ctgctcgccg tagccgttga agatcacggt ttcgcgccgg gcgaagaagt cgggaatccg
      361 gcgctcccgc ttctggctcc atcgcgggtg gtcgacattc ggattgacgt tcgaataaaa
      421 cccgtactcg tgcggcgcag ccttctccca ggccgtcttc ggctggtgct cgacgaagcg
      481 gatgcgcacg agcgacttgc cggacttgaa tccgtacttc cacggcacga ccaggcggac
      541 cggcgcgccg ttctggttcg gcagcacctc cccgtagagg ccaacggcaa ggatcgtcag
      601 cggatgcatc gcctcatcga tcctcaagcc ctcgacatag ggccagtcga gcacgggccg
      661 gcgcaagccg ggcatcgttt ccggctgcag cgccgtgacg aactcgacga atttcgcctt
      721 cgaggtgggc tccacaagct tcgcgatgcg gttgaactcg aagccgatcc atgggatcac
      781 catcgaccag gcttccacgc agcgaagccg gtagacgcgc tcctcgagcg gaaacttgag
      841 caggtcctcg atgccgaagg tccgcggctt cgccacttcg ccgtcgaccg ccactgtcca
      901 cggacgaatg cggaggcgcc tggcgtagtc cgcggggtcg tccttgtcgg tgccgaactc
      961 gtagtagttg ttgtagctcg tgacgtgctt gtacgccgtg ggcggttcgc tcgtgctgaa
     1021 ccggctcatc gcaacgttct ccagtttctg caggccgagc gtcttctcct gcgctgctgc
     1081 cccacgccaa agcgcccccg tggcgccgag gacggacgcg ccggccgcaa tcttcataaa
     1141 acgacggcgg tgctcgtaaa tgcccttcgg cgtgatctcc gagggtggaa catcgttcgg
     1201 ctgcttgata atcatccagg tgagctcctg tcggggccgt aatctgtcgc cacacgagcc
     1261 cataacctta catcgatcca cgagggaacc gccctgcgtg tcctctgccg ggaggcgcca
     1321 gagaacaagt cccgggaacc acccctcttc gtcggcgtca cagcgggagc ggggctcccc
     1381 gtggcgtacc tggatcaccg ccaggagaga tacgtttacg ccgtcggcca aagccccgcg
     1441 atctaaagcg agaggcctcg ctgaactccg caatccgcaa aatcaagcgc catgtgaatg
     1501 agaaggccca cgccagccaa ccgagtggga cgcacgatgg agagaaccgc atatcccgca
     1561 atcgccgggg cggtgtgaag tggatggaat ccaataccgc agcggttggg gtcgtagaca
     1621 gggtcggcga gcagatgatc gaggtctacc agcattgtgg cgagcatgac gagccaggct
     1681 gtgcgccact gtttgcggaa gaagagccaa gctacggccc cgggcactac tgcgtgaaga
     1741 acaaggtgaa caatcgacgc gaacgtcatt acaccgacgt ctcgcaccgc atcatcgcgg
     1801 cgtcagcccc aaggggctgg cgtggcccgc gcctgcgccg tctcatgaat gatgcacgaa
     1861 acgagatcgc ggtcgaactg aggatcgcca tgaagagatc ccaagacctc cgcgcgcaaa
     1921 cccggccact cagccgccct taggccataa atccataggt acgctccacg gtaattgccg
     1981 ggattcccgt actgatctct cagctcgcgg cgaaagtggg tgagcttctt tagatcatca
     2041 gccattcggg ccggttcggc attggctggc ttcacctcca ctatcagaag gttcgacata
     2101 cgacccggta catgaaccag gaagtccggc ttccaaccgc ctcggaccag gcgatggccc
     2161 tgcttgtcca cctcgccgct cagcgagaac ggaaagccgt caggccaacg gttgcgccag
     2221 cgatggtaaa gctcgtagca gtacacccgc tcgcggtatt gaggctcctc cgcaccggca
     2281 accggaagct ggaaatactc cgcgggtatc tctgaggacg ccgaaagcaa ccactcggtg
     2341 accagattca gctcctcgtc cacggcgata gagaacccgg cttaccaaac cttaggcgtc
     2401 cgactgcgaa gcgactttca ccaatggggg ttgcgcccgt tcacgggcac gtatcgccgc
     2461 gtcgcgggcc aaaagcgaag cgaggtaacc ctccaactgc tcgctaccca gtgcgagccg
     2521 tgcgctcttt gcccatgaca gcgtgtggcc ggcgaggatg tccgccaccg taaggcggtc
     2581 tccaaccaga aaagagcgat ttcccaagtc cgtggcaaga atcttcacag ccgtgcggaa
     2641 ttcccagctg gaggtttcaa tcaccgccgg aactcggcgg tccctcggaa gcgcaaatcg
     2701 atgtttcgct attgcccaca gcggcgcgtc gagctcggtc aggacatagc tcatccactt
     2761 gtagcactgg cttcgttcga cggtgccggc cggcggcagc agtcggcttt gcggatgttt
     2821 ctcggccagg tgcatgcaga ttgcggccga ttcagtgatg atggctccat cgtcgtccaa
     2881 gacgggaacc ttcccggcgg ggttgatctc caggaacttc ggctcgcgcc cttcaccgcg
     2941 catgagatcg atctcgacat attcgtattg cgcgccgatc tcctcgagcg cccacaaggc
     3001 tcgaaaggtt cttccgtttc tgtatccgta gagcttcaca ggcctaagcg gtggccggct
     3061 ctttctggag ctgccgggcg gcctggagaa gcgactcctg gtcgagccga gtgagctcga
     3121 tcttcgaggc ccgctcgatt tcaggatagt ttctgccgat ggagcgcgca taggccgggt
     3181 cgtagtgcgc ctccatgagg cgctggaaca gctcgggggc tcgccgttca cgtgcgaggc
     3241 gctcccaaag caagaattcc tcgccgccga cgagtgggcg tagatgcctc aggcgctcca
     3301 tcatcgcgtc gagatcgcgc tcgaagtgcc cgtagtcttc acgccacagc ttgacgcgct
     3361 cgctcatcgg tgcagtgacg gagaagatca tgccggcatg catggcctgc agcagctcgc
     3421 ccggcagttg aatcgcgccg atcttcttgc tttccgattc cacccatact ggccggctgg
     3481 gatcaaagcg acggagcttg tcgacaagga gcgagtcgaa ccacttctgc gtcggctgtg
     3541 atatgccggg gatgtcaccg atgagcgagc cgcgatggct ggcgaggcct tcgagatcga
     3601 gcacttgggc gcctgtctcg gccaaggcat ttagcagtcg cgtcttgccg catcctgtcg
     3661 ggccgcaaag cacgtgatag cggaatttct cggggagctc ggcgagctgc tcgcgaaccc
     3721 aggccctgta cgccttccag ccgccaggca gccgctcgac gcggaatccg atcgtggaca
     3781 gcgcatcgaa ccagagcttg ctgcgcttgc cgccgcggaa gcagtacacg agcatgcgcg
     3841 cgtccttcgg atatcgcgcg accagctctt caatcgagcg tgacatgttc tttagcgcgt
     3901 acgagacgcc gacgaggtat gcgcgatgtt tgtcggtgcg atgcgtcgtg ccgacttccg
     3961 catattcatc gttgtccacg accggcaagt tcacggcaca cggcaaatga tcctcggcgt
     4021 actcgcgctc gctgcgcgcg tcgatcacga gctcgtactg gccctcgagt gcccgctcaa
     4081 ggagctccgg gaaagacctg cgtggatcgg ggtgattgga aggacttgcc atgtacctgc
     4141 ctagcacaaa ggcgcccgcc gctcaatagg aaaacagacg ccgtttttcc taatgtctgg
     4201 cttcgcgccg accgctatcc
//
LOCUS       DASVDU010000251        11059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_216733, whole genome shotgun sequence.
ACCESSION   DASVDU010000251 DASVDU010000000
VERSION     DASVDU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11059
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216733"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(432..632)
                     /locus_tag="VF226_16500"
     CDS             complement(432..632)
                     /locus_tag="VF226_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HEX7075640.1"
                     /translation="MARLLILAGIVLIAAGIIWLVAERVGLGRLPGDIVIERGNVRIY
                     IPLMTMLIISVVLSIVMWLINR"
     gene            complement(647..1270)
                     /locus_tag="VF226_16505"
     CDS             complement(647..1270)
                     /locus_tag="VF226_16505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX7075641.1"
                     /translation="MPAVVTNAEATLDSVAREAAHCRRCPLYKLGTQTVFGAGPADSP
                     VMLVGEQPGDVEDREGLPFVGPAGQMLDRALAAAGLRRDRMYVTNAVKHFKHVQSRGK
                     RRLHQKPNAYEIDRCKWWLDLELDIIDPELTVAMGATAARALAGRPLKVNTLRGEAMT
                     LSGRQMLVTIHPSALLRVRDSESRAAEFARFVDDLRLVGTLVPAVRL"
     gene            complement(1360..2547)
                     /locus_tag="VF226_16510"
     CDS             complement(1360..2547)
                     /locus_tag="VF226_16510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7075642.1"
                     /translation="MRVLDLSHVMAGPVCGLMLADMGAEVIKVEKIDGGDDTRRMLPP
                     DINGEPAAFMMMNRNKRGIALDLKHPRGKEVLKKLVAKADALIENYRHDTMQRLGLGY
                     EALRDINPGLIYCEISGFGRSGPWAEQGGFDLVAQGMSGLMAITGEGPGRAPVKVGAP
                     VTDITAGILAAMGVCAAYAHKLKTGEGQRVDTSLFEAGITHTYWQSAIAFATGVSPGP
                     LGSAHPLSAPYQAFPTKDGWINLGASNQRLWLKFVELLDDPELAADPRFKDNAGRMGN
                     LPALVEALTVHFKKRTTAEWLDLLEKAGIPAGPVLSIREMHAHPQTIAREMVPELEHP
                     VAGKVQTIGLPVKFSKTPGKVAAPAPLHGQHTREVLAELGYGEAEIDSLIAERAVGTA
                     PGH"
     gene            complement(2653..3723)
                     /locus_tag="VF226_16515"
     CDS             complement(2653..3723)
                     /locus_tag="VF226_16515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7075643.1"
                     /translation="MTGEASEDVIIRREGRAGRITMNRPKALNALTYSMTLAIDDALR
                     RWEQDPEVTVVILDGAGGRALCAGGDVRAIYDQGSKASAFARKFWADEYRLNAYISRY
                     PKPYVAFMDGIVMGGGIGLSAHASHRIVTERSQLAMPETGIGLIPDVGGTWLLAHAPG
                     ETGVYLGLVGERMRGAGTMFAGFADTFLPSARLPELAAALGETTDAAAVDSIIKGFSE
                     APPPSDLASQASEIDAAFRFDSVEAIRAALQDMDSDWARKTLSDLDHRSPLALKLTLA
                     AIRNARKLSSLEEALNVEYRLTVRLLEHGEFLEGVRALLVDKDKSPKWNPPRLEEVTD
                     EMVAQFLSPLPESEALALAPPA"
     gene            complement(3855..4409)
                     /locus_tag="VF226_16520"
     CDS             complement(3855..4409)
                     /locus_tag="VF226_16520"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEX7075644.1"
                     /translation="MRRLRGLIASVAFATGLCGAAADQSHADGFIRELRAGALAHDVD
                     GLWSGFSREDGTVDLNGEVIFSPSLPFLWGAIRPALGGTINFDGGTSHAYLDARWEIE
                     TAGGLFFAAGIGAAVHDGDLKLEAPDRKALGSRVLFHIPLELGLRFDQHNSLSVYFEH
                     LSNANLADTNEGMDLLGVRYGYRF"
     gene            complement(4499..5650)
                     /locus_tag="VF226_16525"
     CDS             complement(4499..5650)
                     /locus_tag="VF226_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7075645.1"
                     /translation="MHFALTEEQTAIQETALAFAKERIAPCAAQWDQDSHFPVDVIRE
                     TAPLGMAAIYVPAEKGGSGLSRLDGALIFEALSYGCPAIAAYLSIHNMVAWMVGRYGT
                     DEQIAAWMPKLASMEWLSSYCLTEPGAGSDAAALATRAERQGDHYVLNGTKQFISGAG
                     ATDFYFVFARTGGEGASGVSAFVVFKDTPGLSFGANERKMGWNAQPTRQVIMENVKVP
                     AFNLVGGVEGKGFKFAMSGLDGGRVNIGACSLGPAWAALDKARDYVHERRAFGRQIAD
                     FQALQFKIADMATDLEAARLMIYRAADALDRADPQATMYSAMAKRYATDLGFDIVNQA
                     LQIHGGYGYLKDYGLEKLVRDLRVHQILEGTNEIMRVVISRKVLGGNRG"
     gene            complement(5760..6362)
                     /locus_tag="VF226_16530"
     CDS             complement(5760..6362)
                     /locus_tag="VF226_16530"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075646.1"
                     /translation="MATQVAPLAALHRSRHSLIAVFSLFALLLTTAPGARAESAIEFM
                     KQTARELIAASRTGSQRDFMEVIQKRGHVSAIGLYALGNYKANLQPSDRDTYYGGMVR
                     FIARYAATEAPKYPVSHAEILSPVVREGDGVYVDSRVHLQNGTVYDVRWLLIPQGNSF
                     RVRDAQVMGFWMSPFLKNLFESYIGENGGNPQALVIALNR"
     gene            6564..7457
                     /locus_tag="VF226_16535"
     CDS             6564..7457
                     /locus_tag="VF226_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7075647.1"
                     /translation="MPYADAGGVRIYYEVTGRGTPLVFLHEFAGDHRSWREQVRALSR
                     NFRCITIAARGYPPSDVPEDDASYGQDIANRDVIAVLDAAGIDKAHVVGLSMGAYTTL
                     QLAMHFPDRLLSAVAAGAGTGSDKDTREQFLREAAATADGMEQATRIAAEQICVSSTR
                     VQLQNKDEIGWRMMVEHLADHPPKGSAKVLRNVQGKRPSLYDLEQQLAAVKIPVLLIV
                     GDEDEPCLNVNLFMKRKMPAAQLAMLPGCGHPINHEEPALFSWLVERFLTSVDRGTWQ
                     PRDPRASASLGSSFAAMLSGR"
     gene            8035..9063
                     /locus_tag="VF226_16540"
     CDS             8035..9063
                     /locus_tag="VF226_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7075648.1"
                     /translation="MSLAGELEIEEAVAGLKAAADQTRLRILLLLASGELNVKDLTRI
                     LGQSQPRISRHVKLLGEGGLIERVREGSWVFCRLADGPRGDLARAIVQSIDTTDSVYV
                     RDLQRAEALKAERAAAAQAYFRTHAADWDRIRALHIPEVEVETAMREALGDDRAELLI
                     DLGTGTGRTLEIFSDIYDRGVGIDLNQSMLAYARAKLLREGLDHAQVRHGDIYNLSFP
                     DGVADVIVMHQVLHFLSDPGRAIREAARVLSPGGRLLIVDFAPHQLEFLRDEFAHQRL
                     GFSSAQVRQWLGEAGLVLEHSRDLPPPAGREDGKLTVSLWLASRPKVRRGSASRSSEA
                     VQSLETSP"
     gene            9060..9974
                     /gene="metF"
                     /locus_tag="VF226_16545"
     CDS             9060..9974
                     /gene="metF"
                     /locus_tag="VF226_16545"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX7075649.1"
                     /translation="MTPERKSRLLGAGEISVSFEFFPPKNERMEEALWAAVRRLEPLS
                     PGFVSVTYGAGGSTRERTHATVARIVRETSLQPAAHLTCVGASRCEVDAVARAYWQAG
                     VRHIVALRGDPPAGVGARYEPHPGGYANAAALVAGIASIGAFEISVAGYPEKHPESPS
                     VAHDLDNLKAKVDAGASRVITQFFFDNTQYLRFVENARAHGIWCPIVPGIVPIHNFKQ
                     VAGFAAKTGASIPAWLARRFDGLDDDPATTHLVAAAVAAEQVMDLVDQGIRQFHFYTL
                     NRADLVFAICHLLGLRPRTAAVQQAVSA"
     gene            9971..11023
                     /locus_tag="VF226_16550"
                     /pseudo
     CDS             9971..11023
                     /locus_tag="VF226_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131456.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
BASE COUNT         1979 a         3514 c         3596 g         1970 t
ORIGIN      
        1 cctttggatg taagcccttg tcctgggcgt ggtggtggcg ttggcgtgca agctcagctc
       61 catgacccga ggcgctccct gacgaaggcg ataccttggg ctacaccgat tcggtcgatc
      121 caatcctccg ggactgaaca cctgcgcggg gatgacggta tggggagcgg tatcgccgat
      181 ccccgtcttc aagggcgctc gtcgccggga tccatctgac cgcaggtgaa ggccccgccg
      241 ctttgcactg gcggtgcgac aacccaaact gtcgagttgg ttcatgggtg gaccccgggg
      301 cataaagccc ggggtgacaa ggtataggga ggacgggcga cgttttctga caccgtcact
      361 gagccgatcg agtagccctg tcgtttacgc tcccccacca ctcgctattc gctattcgcc
      421 tttcgctcgc actaccgatt gatcagccac atcacaatgc tcaggacgac gctgatgatg
      481 agcatcgtca tgagcgggat gtagatgcgc acgttgccac gctcgatgac gatatcgccg
      541 ggcaggcggc cgaggccgac acgttcggcg acaagccaga tgatgccggc ggcgatcagg
      601 acgatgccgg ccagaatcag aagccttgcc atttcaggcc ctccgatcat aggcgcacgg
      661 ccggaacgag ggtgcccacg aggcgcaggt catcgacgaa gcgggcgaac tccgccgccc
      721 ggctctccga atcgcgcact ctcaatagcg cggacggatg aatggtcacg agcatttgcc
      781 gtccagagag ggtcatggcc tcacctctga gcgtgttcac cttgagtggc cggccggcca
      841 gcgcgcgggc ggctgtcgcc cccatcgcga ctgtcagctc gggatcgatg atatcgagct
      901 cgagatcgag ccaccacttg cagcggtcga tctcgtaggc gttgggtttc tggtgcagac
      961 gccgcttgcc gcggctctgc acgtgcttga agtgcttcac cgcattggtc acgtacatgc
     1021 gatcgcgccg caggcccgcg gcggcgagcg cgcgatcgag catttggcca gctggcccca
     1081 cgaagggcag accttcgcgg tcctccacgt ctccgggctg ctcgccgacg agcatgaccg
     1141 gcgaatcggc cggtccagcg ccgaacacgg tttgcgtgcc aagcttgtaa agcgggcagc
     1201 gccggcaatg cgcggcttcg cgcgccacgc tatccagtgt ggcttcggca ttggtgacaa
     1261 ccgcgggcac ggtcctgctc ccgccatcca gcctctccaa gtttcaaccc gtggccggtg
     1321 ggaatggttc agaacgggcg tcccgactta cccagggtat cagtgtcccg gcgctgtccc
     1381 gacggcgcgt tccgcgatca ggctgtcgat ctcggcctcg ccgtaaccca actcggccag
     1441 aacttcgcgc gtatgctgcc catgcagcgg agcgggtgcc gcaaccttgc ccggcgtctt
     1501 ggagaatttc accggcaggc caatggtctg caccttgccg gcgacgggat gctccagctc
     1561 cggaaccatc tcgcgcgcga tggtctgcgg gtgagcgtgc atttcccgga tcgagagcac
     1621 cggcccggcg gggatgcccg ctttctcgag caggtcgagc cattcggccg tggtgcgctt
     1681 cttgaagtga acggtgagcg cctcgaccag agcgggaaga ttccccatcc ggcctgcatt
     1741 gtccttgaat cgcggatcgg cagccagctc cggatcgtcc agcaattcga cgaacttgag
     1801 ccagagccgc tggttggagg ccccgagatt gatccagccg tccttggtcg ggaatgcctg
     1861 ataaggcgcg gacagcgggt gcgcagagcc gagcggaccc ggagaaacgc ccgtcgcgaa
     1921 ggcgatggcc gactgccagt aggtatgcgt gatgcccgct tcgaaaagtg aggtgtcaac
     1981 ccgctggcct tcacccgtct tgagcttgtg ggcgtaggcg gcacaaacac ccatcgccgc
     2041 aagaattccc gccgtgatgt ccgttacggg cgcgccgacc ttcaccgggg cacggcctgg
     2101 gccttcgccc gtaatggcca tcaggcccga catgccctgc gcgacgaggt cgaagccgcc
     2161 ctgctcagcc cacggcccgc tgcggccgaa cccggagatc tcgcagtaga tcaggccggg
     2221 attgatatcc cgcagcgcct cgtagcccag cccgagccgt tgcatcgtgt cgtggcggta
     2281 gttctcgatg agcgcatcgg ctttggccac gagcttcttc agcacctcct tgccccgcgg
     2341 gtgcttcaag tcgagcgcga taccccgctt gttgcgattc atcatcatga aggccgccgg
     2401 ctcgccattg atgtcgggcg gcagcatgcg gcgggtatcg tcaccaccgt cgatcttctc
     2461 gaccttgatg acctcggcgc ccatgtcggc cagcatcagc ccacagacgg gacccgccat
     2521 gacgtggctg aggtcgagca cccgcatgcc cttgagcggt cctgtgcgtt ctgtggtgtt
     2581 cacggcttat ctgctccccg aaacattgaa ggttaggcgg cgaaggaacc tagcgcgttc
     2641 ggtgcgcgtg atctaggcgg gtggcgcgag cgccagggcc tcggattccg gtaatgggct
     2701 gaggaactgc gcgaccattt cgtcggtgac ctcctccagc cgcggcgggt tccatttcgg
     2761 cgatttgtcc ttgtcgacga ggagggcgcg aacgccttcg aggaactcgc cgtgttcgag
     2821 aaggcgcacc gtcaggcgat actccacgtt cagcgcttct tccagcgagg agagtttgcg
     2881 tgcgttacgg atggccgcca gcgtcagctt gagggcgaga ggcgagcgat ggtcgaggtc
     2941 ggacagcgtc ttgcgcgccc agtccgaatc catgtcctga agcgcggcac ggatcgcctc
     3001 gacgctgtcg aacctgaacg cagcatcgat ttcagaagct tgggaggcaa gatcggaagg
     3061 cggcggcgct tcgctgaagc ctttgatgat gctgtcgacg gccgcagcat ccgtcgtctc
     3121 gccgagggcc gcagcaagct ccggcaggcg tgcggaaggc aggaaggtat cggcaaagcc
     3181 ggcgaacatc gttccggctc cgcgcatgcg ctcgccgacg aggcccagat agacacccgt
     3241 ctcccccggt gcgtgcgcca gcagccaggt gccgccaaca tccgggatga gaccgatgcc
     3301 cgtctccggc atggcgagct ggctgcgctc ggtgacgatg cgatggctcg cgtgggctga
     3361 aaggccgatt ccgccgccca tcacgatgcc gtccatgaag gcgacgtacg gcttcgggta
     3421 ccggctgatg taggcgttga ggcgatactc gtcggcccag aacttgcgtg caaaggcgga
     3481 tgccttcgag ccttgatcgt aaattgccct gacatcgcca cccgcacaca atgcgcggcc
     3541 gccggctcca tcgagaatga cgaccgtgac ctcgggatcc tgctcccagc gcctcagggc
     3601 atcgtcgatg gccaaggtca tcgaataggt gagcgcattg agcgccttcg gccggttcat
     3661 ggtgatgcgc ccggcgcgtc cttcgcgccg gatgatgacg tcctcggaag cctctccggt
     3721 catgcctcgt ccttcagcaa ttctggcagg cgccctcggc cctgcaaccc tcaggtcgtg
     3781 tgggcgatct cgcaactgaa tgcaagacaa gaggttgccg cagaaacgtc cgaagctgga
     3841 cctgagcggt cgcgctagaa cctgtagccg tagcggacgc cgagcaagtc catgccctcg
     3901 ttcgtgtcgg cgagattggc gttcgacagg tgctcgaaat agaccgaaag gctgttgtgc
     3961 tggtcgaaac ggaggccgag ctcgagcggg atgtggaaca gcacacggct gccgagcgcc
     4021 ttgcggtccg gcgcctcgag cttcagatcg ccgtcgtgca cggcagcgcc tatgccggct
     4081 gcgaagaaga gcccgccggc cgtctcgatt tcccagcggg cgtcgaggta ggcatggctg
     4141 gtgccgccgt cgaagttgat cgtgcccccg agagccggcc ggatggcgcc ccagagaaag
     4201 ggcagggatg gcgagaagat cacctcgcca ttgagatcga ccgtgccatc ttcgcggctg
     4261 aacccgctcc agaggccgtc gacatcgtgg gcgagggcac cagccctcaa ctctcgaatg
     4321 aatccatcgg cgtgcgattg atctgccgcc gcaccgcata agcctgtcgc gaacgcgaca
     4381 gacgcgatga gccccctcag ccgacgcatt tccaccagcc ccccaactgc gcagacgacg
     4441 tcacattggc gacggtgtcg ggccagggca agggcgaact ggaacgacct gagaaatttc
     4501 agcccctgtt accgccgagc accttccggg agatcacgac gcgcatgatc tcgttcgtcc
     4561 cctccaggat ctgatgcacg cggagatcgc ggacgagctt ctccagcccg tagtccttca
     4621 ggtagccata tccaccgtgg atctgcagcg cctggttgac gatgtcgaag ccgaggtcgg
     4681 tcgcgtagcg cttcgccatg gccgagtaca tggtggcctg cggatcggcg cggtccaggg
     4741 cgtcggcggc ccggtatatc attagccgcg cggcttcgag gtcggtggcc atgtcggcaa
     4801 tcttgaactg gagagcttga aaatctgcga tctggcggcc gaaggccctt ctctcgtgca
     4861 cataatcgcg cgccttgtcg agggcggccc aggccggacc cagtgagcag gcgccgatgt
     4921 tgacgcggcc gccgtcaaga ccgctcattg cgaacttgaa ccctttgccc tcgacgccgc
     4981 cgacgaggtt gaaagctgga accttcacgt tctccatgat gacttggcgg gtcggctgcg
     5041 cgttccagcc catcttgcgc tcattggcgc cgaaggagag gccaggggtg tccttgaaca
     5101 cgacgaacgc cgagacgccg gaagcgcctt cgccgccggt gcgcgcgaac acgaagtaga
     5161 agtcggtggc acctgcaccg gagatgaact gcttggtacc gttcagcacg tagtgatcgc
     5221 cctggcgttc ggcacgggtg gcgagcgccg cggcatccga gccggcgccc ggctccgtca
     5281 ggcagtagct cgacagccac tccatgctgg caagcttcgg catccaggcc gcgatctgtt
     5341 catcggtgcc atatcggccg accatccagg cgaccatgtt gtggatcgag aggtaggcgg
     5401 ctatggccgg gcaaccgtac gacagcgcct cgaaaatgag ggcgccgtcg agccgtgaca
     5461 gccccgagcc gcccttctcg gcgggaacgt agatggccgc catgccgagc ggcgccgtct
     5521 cccggatcac atcgacggga aaatgggaat cctggtccca ctgcgccgcg catggcgcaa
     5581 tccgttcctt ggcgaaagca agggcggtct cctggatcgc ggtctgttct tcggtcaggg
     5641 cgaaatgcat cgctaggcct cgtcgtgctt catgcgcgga agcctagcgc gatcagacgc
     5701 gcgatgaaac agagctcagg gcgacgagag ctcctgccgc accccatcgg gcgccagggt
     5761 cagcggttca gcgcaatcac cagcgcctgg gggttgccgc cgttctcgcc gatgtagctc
     5821 tcgaacaggt tcttgaggaa ggggctcatc cagaagccca tgacctgtgc gtcgcgcacg
     5881 cggaacgagt tgccctgggg tatgagcagc cagcggacat cgtagacggt gccgttctgc
     5941 aggtgcacac ggctgtcgac gtagaccccg tcaccttccc gaacgacagg cgagaggatc
     6001 tcggcatggc tgacggggta tttcggtgct tccgtcgccg cataccgcgc aatgaaacgg
     6061 accatgccgc cataataggt gtcgcgatcc gacggctgca ggttcgcctt gtagttgccg
     6121 agcgcgtaca gcccgatggc cgaaacgtgc ccacgtttct ggatcacctc catgaagtcc
     6181 cgctgcgagc cggttcgaga agcggcgatc agctcgcgtg ccgtctgctt catgaactcg
     6241 atggcagact ccgcccgggc gcccggagca gtggtcaaca gtaatgcaaa cagcgagaaa
     6301 actgcaatca agctgtgccg gctgcgatgc aacgctgcaa gcggtgcaac ttgagtggcc
     6361 attccatttc tcccgaacaa gaagcggact gacatgaaca tgtttcgggc cccgagcagc
     6421 caagcacgtg ccacaatcaa ttggcagccg tcgctctccc cgtcaactgc cgccggtgga
     6481 taccggtgga gcctcggcac acttcatgtc tccacggtca cggtgtacaa agcgttacca
     6541 gattgatgga agggaaacga gtcatgcctt atgcggacgc cggaggagtt cggatttact
     6601 atgaagtaac cggccgcggc acgccgctcg tcttcctgca tgagttcgct ggtgatcacc
     6661 ggagctggcg cgagcaggtt cgcgcgctca gccggaactt ccgctgcatc accattgcgg
     6721 cgcggggata tccgccgtcg gacgtgcccg aggatgatgc atcctacggc caggacatcg
     6781 caaatcgaga cgtgatcgcc gtgctggatg cggcgggaat cgacaaggcg cacgtggtcg
     6841 gcctcagcat gggcgcctac acgaccctcc agctcgccat gcatttcccg gaccggctgc
     6901 tgtcggcggt tgccgcgggg gcagggaccg gctccgacaa ggacacgcgc gagcagttcc
     6961 ttcgcgaggc agctgcaacc gcggacggca tggagcaagc gacccgcatc gctgccgaac
     7021 aaatctgcgt gagctcgacc cgggtgcagc tgcaaaacaa ggacgagatt ggctggcgca
     7081 tgatggtgga acatctggcc gatcatccgc cgaagggctc ggcaaaggtg ttgcgcaatg
     7141 tgcagggcaa gcggccgtcg ctctacgacc tcgagcagca gcttgcggcg gtcaagatcc
     7201 cggtgctgct gatcgtgggc gacgaggacg agccttgcct caatgtcaat ctgttcatga
     7261 agcggaagat gccggccgct cagctcgcga tgctgccggg ctgcggccac cccatcaatc
     7321 acgaggagcc ggcgctgttc tcgtggctcg tcgagcggtt cctcaccagt gtggatcggg
     7381 ggacctggca accgcgcgat ccgcgggcca gtgcctctct cggcagttcc ttcgcggcaa
     7441 tgctcagcgg tcgttgagtc ggcaatcggg cgtcgggtcg cgcgggctcg atcgcccggt
     7501 ggtagccgat cggggcagtc tgcggcccat ccgtcacaca ccgcacctgc actggcgggg
     7561 tcgcgagccg gacgaatggc agcccgcaga acagcagcga catgacgagc gccgccgcga
     7621 gctgcacggc aacgagcggc ttttcgacca ggaccttcag catgtccggt tgttccgcag
     7681 cggctgcggc aacgatcccg aatggtgtca agagaagcgc tgccgccggc aggacgagaa
     7741 ggctcgttgc caaccggccg accgcgggcg agcgttggtt ggaaaattgg ttccggttca
     7801 aactgttgga cgtcgtcatg ggcggcgttc ttgcaaaaga catcaaggaa tgcgtggggt
     7861 gagttggagg agatcggctc gaagccatca gcactggctc tcccatatca gatgtgctct
     7921 tgcccgcctg ttacgccggg aacaggattt tgccgctcag tgttaacccc tcgtaattca
     7981 tcttgacgca tataaagata tctttatatg ctcgccttga catggaggca gaggatgtcg
     8041 cttgcgggtg agctggagat cgaggaggcg gtggcggggc tgaaggccgc cgcggatcag
     8101 acgcgtctgc gcatcctcct gctgctcgcc tcgggcgagc tcaacgtgaa ggacctcacc
     8161 cgcattctcg ggcaaagcca gccgcgcatc agccgccacg tgaagctgct tggcgaaggc
     8221 gggctcatcg agcgtgtccg cgaggggagt tgggtgttct gccgactggc cgacggcccg
     8281 cgcggcgatc ttgcccgcgc gatcgtgcaa tcgatcgaca cgacggattc cgtctacgtc
     8341 cgcgatcttc agcgggcgga ggcgctcaaa gccgagcgcg cggctgcggc acaggcctac
     8401 ttccgcaccc acgccgcgga ctgggaccgc attcgcgcgc tgcacatccc agaggtcgag
     8461 gtcgagacgg ccatgcgcga ggcgctgggg gatgatcggg cggagctgct gatcgatctc
     8521 ggcaccggca ccgggcgcac gctcgagatc ttctccgata tctacgatcg cggcgtgggc
     8581 atcgacctca accaatcgat gctcgcctat gcgcgcgcga agctgctgcg cgaggggctg
     8641 gatcatgccc aggtccggca tggcgatatc tacaacctgt cgttcccgga cggtgtcgcc
     8701 gacgtcatcg tcatgcatca ggtgctgcat ttcttaagtg acccgggccg ggcgatcagg
     8761 gaggcggcgc gcgtgctgtc tcccgggggt cggctgctca tcgtcgattt cgccccgcat
     8821 cagctcgaat tcttgcggga tgagttcgca caccaacgat tgggcttctc ttccgcccag
     8881 gtccggcaat ggctgggaga ggcgggcctt gtgctcgagc acagtcgcga tctgccgccg
     8941 cccgcaggcc gggaggacgg caagctcacc gtatcactct ggctcgcaag ccgcccgaag
     9001 gtgcgcaggg ggagcgcttc tcgcagctcc gaagcagtac agtccctgga gacgtcgcca
     9061 tgacccctga gcgtaagagc cggctcctcg gcgccggcga gatttcggtc tccttcgagt
     9121 tcttcccccc caagaacgag cggatggaag aggcgctgtg ggcggccgtg cgccggctcg
     9181 agccgctgtc gcccgggttc gtctccgtca cctacggtgc gggtggctct acgcgcgagc
     9241 gcacccatgc caccgtcgcc cgcatcgtcc gcgagacgtc gctgcagccc gctgcgcacc
     9301 tcacgtgtgt cggcgcaagc cggtgtgagg tcgatgccgt ggcgcgggcc tattggcagg
     9361 ccggcgtgcg ccacatcgtg gcgctgaggg gcgatccgcc ggcgggcgtc ggcgcgcgct
     9421 acgagccgca tccagggggc tatgccaatg cggctgcgct ggtcgcaggc atcgcgagca
     9481 tcggcgcctt cgagatctcg gtggccggat atccggaaaa gcacccggaa agcccctcgg
     9541 tcgcgcacga tctcgataac ctgaaggcca aggtggatgc gggcgcgtcc cgcgtcatca
     9601 cgcaattctt cttcgacaac acccagtatc tgcgcttcgt cgaaaacgcc cgtgcccatg
     9661 gcatctggtg cccgatcgtg ccgggcatcg tgcccattca caatttcaag caggtcgccg
     9721 gatttgccgc gaaaacggga gcgagcattc cggcctggct tgcccggcgc ttcgatgggc
     9781 tcgacgatga tccggcgacg acccatctgg tcgcggcggc cgttgcggcc gagcaggtga
     9841 tggatctggt cgaccagggc atccgccaat tccatttcta tactttgaac cgcgcggacc
     9901 tcgtgttcgc catctgtcat cttctggggc ttcggccgcg tacggcggcg gtgcagcagg
     9961 cggtcagcgc atgagcaaac gacaaaagag aattgcggcg ctccaggccg ctgcggccga
    10021 gcgcatcctc atcatcgacg gtgcgatggg cacgatgatc cagcagcaca agctgggcga
    10081 ggcggactat cgcggcaagc ggttcgccaa tcaccatcgt gacgtgaagg gcaacaacga
    10141 cctgctggtc ctcacgcagc ccagcatcat tctcgacatc cacaatcaat atctcgctgc
    10201 cggcgccgat atcatcgaaa cgaatacgtt caacgcccag cgcatttcgc ttgcggatta
    10261 cggcatggaa gggctggcgt atgaaatcaa cgttgcggca gctcagctcg cgcgcgaggc
    10321 ggccgatatg tggacgttga aaacgccgaa ccgcccgcgt ttcgttgcgg gtgccgttgg
    10381 cccgaccaac cgcacggcat ccatttcacc cgacgtgaac aatccgggat accgcaatat
    10441 caccttcgac gagttgcgcg aggcttacgc agagcaggtc aggggcctga tcaagggtgg
    10501 cgtcgacatc atcctgatcg agaccatttt cgacacgctg aatgccaagg cggcaggatt
    10561 tgccacgctt gaagtgttcg acgaggtggg cttctcgctg ccgatcatga tctcgggcac
    10621 gattacagat cgctcgggcc gcacgctttc cgggcagacg accgaggcat tctggtattc
    10681 gatgcgccat ctcaagccat tcgcggtcgg cctgaactgc gcgctggggg ccgaggcgat
    10741 gcggccttat ctggccgagc tcgcggctgt ggccgatacg ctgatctccg cctatcccaa
    10801 cgctgggttg cccaatgcga tgggcgagta cgacgagacg ccggacgaga tggcctacca
    10861 gatcgagtcc tgggcccgcg acagcctcct caacatcgtc ggcggctgct gcggctcgac
    10921 gccggagcac atcaggcacg tccgcgagca cgtcgagaaa tacccgccgc ggaaagtgcc
    10981 aaagctcgag ccgcgaatgc gcctgtcggg cctcgagccg tttgtgcacg ggtgagcgga
    11041 tggaagccat ccgcgtgga
//
LOCUS       DASVDU010000252         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1603207, whole genome shotgun sequence.
ACCESSION   DASVDU010000252 DASVDU010000000
VERSION     DASVDU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1603207"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="dctP"
                     /locus_tag="VF226_16555"
     CDS             complement(<1..653)
                     /gene="dctP"
                     /locus_tag="VF226_16555"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7075650.1"
                     /translation="MNKAANLFRTTVFSVGALALSGVIALAQDGPPDKVTEWVFQPAF
                     DQTDAGWDKGLIPWVKAVEEATKGTVKIRVEPAGALTSASEAFAAAAAGQTDGYAGWA
                     TVYGGEMPEGSLAYGLPLGAANSEEAWKVMWGDPKYRIGELVQEAAHERNLHWVGWTN
                     QGPNAMFTKFRVDRLEDLAGKKMRAGGPQAIFHSTMGGSPVSMNAGEIYTALKLGTI"
     gene            complement(741..1238)
                     /locus_tag="VF226_16560"
     CDS             complement(741..1238)
                     /locus_tag="VF226_16560"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7075651.1"
                     /translation="MEKLIDAITEVMGRIGWLLILYCMIFGVTDVFLRYALNAPSLWI
                     GTTLQAAMVLIACTGGAYALKHDAFVKLDVFYASVSTRNKAVLDVLTAPFAILFLSVL
                     IWKGIDAALLSIKFNQVTPTAVRIPIYPIKSVIPLAAAVVLLLVVKQLVRNVRTIFSG
                     EQAAE"
     gene            1486..1698
                     /locus_tag="VF226_16565"
     CDS             1486..1698
                     /locus_tag="VF226_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075652.1"
                     /translation="MRPASSQSNDLESSSAPSVVKSANASSCAAAGKQLRPGRGALGR
                     HANISDDAADITGQPSAPLAGLTMAG"
     gene            1712..>2175
                     /locus_tag="VF226_16570"
     CDS             1712..>2175
                     /locus_tag="VF226_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7075653.1"
                     /translation="MQYEDILYEAADGIARITINRPNVMNAFRGKTCEELIHAFNRAG
                     WDSSIGVIVLAGAGDRAFCTGGDQSAHDGKYDGRGMIGLPVKELQTLIRDVPKPVIAR
                     VQGYAIGGGNVLATICDFTIASEKAIFGQVGPKVGSVDPGFGTAYLARIVGE"
BASE COUNT          435 a          686 c          626 g          428 t
ORIGIN      
        1 tcgatcgtgc caagcttgag ggcggtatag atttcgcctg cattcatcga caccggagag
       61 ccgcccatcg ttgagtggaa aatggcctgc ggcccgccgg cacgcatctt cttgcccgcc
      121 aagtcctcca gccggtccac gcggaacttg gtgaacatgg cattcgggcc ctgatttgtc
      181 cagccgaccc aatgcagatt gcgctcgtgt gcggcttcct ggaccagctc gccgatccgg
      241 tacttcggat caccccacat gaccttccag gcctcttcgg agttggcggc ccccaacggg
      301 agaccatagg ccagcgagcc ttcgggcatc tcgccgccgt agacggtggc ccagccggcg
      361 taaccgtccg tctgaccagc tgcagcggcg gcaaacgcct cggaagcgct cgtcagcgcg
      421 cccgcgggct cgacacggat cttcaccgtg cctttggtgg cttcctctac cgctttcacc
      481 cagggaatga gccctttgtc ccatccggca tcggtctggt cgaatgctgg ctgaaacacc
      541 cactccgtca ctttgtccgg cggtccgtcc tgggcgagcg caatcacacc ggataacgcc
      601 agagctccaa cggaaaatac tgtcgttctg aagagatttg ctgccttgtt cacgagacca
      661 ttcctcccta gctcgtcgaa tttgcgaatg tcgaatttgc gaatattgtt ctgccggccc
      721 ttttcctttc ttcctcgtcc tcactctgcc gcttgctcac cgctgaagat cgtccgaacg
      781 ttgcggacga gttgcttcac gaccaggagc agcacgaccg cggcggccaa tggaatgact
      841 gatttgattg gataaatagg aatccgcacc gctgtcggcg tgacctgatt gaacttgatg
      901 gacaggagcg ccgcatcgat ccccttccag atcaggacag agagaaagag gatcgcaaaa
      961 ggcgccgtca gaacgtccag caccgcctta ttccgcgtcg acacactggc atagaagacg
     1021 tcgagcttga cgaaggcatc gtgcttcagc gcgtacgccc cgccggtaca ggcgatcagc
     1081 accatggcgg cctggagcgt cgttccgatc cacagggagg gggcgttcag ggcgtagcgg
     1141 agaaaaacat ccgtcacgcc aaatatcatg cagtacaaga tcagaagcca gccgatcctg
     1201 cccatgacct cggtaatggc gtcgatcagc ttctccaatc cgcgccatag cttcatgggc
     1261 cgctggctcc tcccttatga gcgggacatc atatcttgaa aattatggtg cgggccactg
     1321 ctgcctgtca acgcccagca tgagctgcca aaccggcaac gtgcttgact cactttggat
     1381 tctctcctct gtcgattggc gagcacccct cgacagcagg acggaagcgc tcagccgctt
     1441 ggctcgtact ttgccgggag acgatccggg agctcgcaca agcgcatgag gccggccagc
     1501 agccagtcca acgacctgga gagcagcagt gctccgtccg tagtgaaatc cgccaatgcc
     1561 agctcatgcg cggctgcagg caagcagctt cgcccaggtc gcggcgccct tggccgtcat
     1621 gcgaacatca gcgatgatgc cgccgacatc accggccagc cgtcagcgcc tctggcgggg
     1681 ctgacgatgg ctggctgaga ggagctttca gatgcagtac gaggatattc tttacgaggc
     1741 cgccgacggt atcgcccgca tcaccatcaa tcgcccgaac gtcatgaacg ctttccgcgg
     1801 gaagacctgc gaagaactca ttcacgcgtt caaccgcgcg ggctgggact cgtctatcgg
     1861 cgtcatagta cttgccggcg ccggcgacag agcattctgc accggcggcg atcagtcagc
     1921 tcatgatggc aagtatgatg gccgcggcat gatcgggctg ccggtcaaag agctgcagac
     1981 cctgattcgc gatgtgccga agccagtcat tgcgcgcgtg caaggctatg ccatcggcgg
     2041 cggaaacgtg ctcgccacga tatgcgattt caccattgcc tccgaaaaag cgattttcgg
     2101 acaggttggc ccgaaagtcg gctcggtcga tccgggattc gggacggcat atctggcccg
     2161 tatcgtcggt gagaa
//
LOCUS       DASVDU010000253        13173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1221913, whole genome shotgun sequence.
ACCESSION   DASVDU010000253 DASVDU010000000
VERSION     DASVDU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221913"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF226_16575"
     CDS             <1..195
                     /locus_tag="VF226_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075654.1"
                     /translation="GREAVSIEPNVFARLTLAIVDLRIDLPEKKIRCCKGKCSQGNRN
                     RNIGPAAMHSLGQMKSWADE"
     gene            599..2695
                     /locus_tag="VF226_16580"
     CDS             599..2695
                     /locus_tag="VF226_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012559899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7075655.1"
                     /translation="MRAGSEVRVAVDVGGTFTDIALMADNGVLHESKISTTPDDPSIA
                     VVDGLIALLDELKIAPSAVAEVLHGTTVGSNTILQRKGARTGLITTRGFRDVLEIGRI
                     RMPDMFDLTWDKPKPLVPRRHRLEVTERIAADGSVIEPLSEGDVISAAEALLADGVEA
                     IAICFLNSYRNPAHEQQAEAILKARFPDLAVSASYAVLPEMKEYERTSTTVVNAYLLA
                     AMRSYLRRLEQRLAAAGIAAPILVMTSNGGMLPAATAGDKPVMVVASGPAGGVIGAAR
                     LGAALSETDIIVFDMGGTTAKAVIIEDGRPSMTSEYEFRDGMSTSSRFIKAGGYMLKV
                     PAIDIAEVGAGGGSIAGIDAGGLLKVGPESAGAVPGPACYSLGNTRPTVTDANVVLGM
                     VNPKSLAGGRLAIDRRLAEEAIREHVARPLGLSLEDAAHGIRAVANAAMARAIRAVTV
                     ERGRDPRDFTMMAFGGNGGNHGVDVARQLGVRKIIIPPFAGVFSAVGMLAADVEHTAL
                     ATVMRLLDTITPTELGEIMDGLAADVSARLAAEGYSGNKAELAWQAEVRHEGQATELP
                     VPFEATGDRFAQIRERFFAEYFKTYGYRDQTPLELVKVTVVGRGLRRNRLDFSNMRID
                     QRPGAARSGHRLVSFTRGEPAIETEIVPRAAVGSEPRRGPLIVEEFDATIVVPPDTSI
                     SRDRTGNIVLEIGGQA"
     gene            2692..4407
                     /locus_tag="VF226_16585"
     CDS             2692..4407
                     /locus_tag="VF226_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7075656.1"
                     /translation="MNVDPITFAVIKSGLDSIADDMAYTVVRIARSEIVKDVMDFSAA
                     ICAADGQMVAQAKTIAQHLGAIPQAMEAVLAKYGDDLHDGDVVIMNDPYHGGMHLPDI
                     FMFVPLFYEGRRRAFAVVICHHTDVGGRVPGSNASDSTEIYQEGLRLPPLKLYERGKL
                     NETLETLIKINVRVPDRVWGDLSAQYAAAQVGKRGLDRLFHRYGADEVEAYMQELLDY
                     AERLTRAEIRQWPKGTYRFTDHIDNDGFSDKPIPISVAITVRDDGTLLVDYTGSSPQV
                     KGALNSTLSFTHSLTYLSVRCVLGRDVPNNVGVFRCLEIKVPEASVLNPVMPGPCAAR
                     ALTGYRVFDTMLGALAQIVPDRVPAAGEGGNSVICISGLRPNRKPFIIVDMICGAWGG
                     RPDKDGLEAVTNASQNLSNMPVEVMEAEHPVRVEEYAFVPDSCGAGHWRGGIGLRRSY
                     RILAPEALLQMRTDRVAFRPYGLAGGEPGGPSRNSIEIDGEVKPLPGKVTMTVPQGAV
                     IVHDQAGGGGFGNPFTRDPELVLEDVKDGKITAEFARDRHGVVITAGRLDLEATQRLR
                     QERVA"
     gene            4404..5162
                     /locus_tag="VF226_16590"
     CDS             4404..5162
                     /locus_tag="VF226_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7075657.1"
                     /translation="MTRGRAHSMNASVELQPKRPVKATRSAEASRAKILDAARYEFVT
                     HGLSGARVDRIAERAGVNKNLIYHYFGSKDDLYLRILEEIYSDLRASQRDLQVRDLPP
                     IEGMRCLVGRTFDHFVATPALIRLMSIENIHYARYLKRSKLVKPLYTPLLDTIEHLLA
                     RGQADGVFRRDVDAVDLYISISGLAYFYLSNRHTLSWIFDQQFDSPKRLAQRRAHIVD
                     MVLGYLQHKGSVVDEAIAASRDQTSPHAKSTAER"
     gene            5189..6169
                     /locus_tag="VF226_16595"
     CDS             5189..6169
                     /locus_tag="VF226_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075658.1"
                     /translation="MRRRELLALAAAGAIALASPVGANAQEKLTVRLDFSPWGVQAGM
                     HLAKEKGWFAAEGLDVEIQDGRGSGNTVQLVNAGQVDVGQVQVGLVASAREQGGKIKS
                     IFNFQRKTDLCILVDRDSPIKTVADLKGKTVVVFAASPWAPFIDLYLKSGGMDRETVK
                     VEFVDPAALWGTYTARRADGLMSTVGSALPVAEKPRPSKCLLASDAGIGFPAYGLVAH
                     EDTIASKRDALKKLIKIQQRAWEHLRTNIDDGVKAMIAQRPDAKLDPEVLAGQIKLTI
                     EYFDTPATAGKPIGWQAKADWEQALKSMEQAGVVKPGWNVDDYFTNDLIE"
     gene            6176..6961
                     /locus_tag="VF226_16600"
     CDS             6176..6961
                     /locus_tag="VF226_16600"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075659.1"
                     /translation="MAAAAEAYVTMSRVGKVYPSQQGDVVALTDFDLEIRRGEFLSIV
                     GPSGCGKSTLLKCVAGLEPVSAGTMAVNGQPVQGPPDQLGVVFQRDVLLDWRTILDNV
                     LISAEFLGRRRADLKARAIELLQRFGLGGFEDRHPWELSGGMRQRAAICRALLCDPEL
                     LLMDEPFGALDAMTRDDLNVELTRIWQDSRKTVLFITHSIVEAVYLSDRVVMMSRAPG
                     RIVDIIDIDIPRPRPLSVRESKRFGELTSKIRHHFAELGIVKE"
     gene            6958..7761
                     /locus_tag="VF226_16605"
     CDS             6958..7761
                     /locus_tag="VF226_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075660.1"
                     /translation="MKRGIGDWFKTESRTRDVLLVLAASIAIWELGVQVLAPSPLILP
                     APSAIAREFMTTPGIFIRHLGFTLAVTVAGFLLALVMGVALAVGIVYSRFLERTVYTL
                     LVALNSIPKVALAPLFVIWLGTGTEPKLAVAVMLAIFPIVIDTVLGLRSVDPDMLNLA
                     RASRASAWQILFKIRFPCALPSLFAGMKVAVSFALVGAIVGEFVAGGRGLGFQILVAQ
                     GQFDTPRVFVSLLLLGIMGTILFYAVDFAERVCLPWHVSQRTQLARLDH"
     gene            complement(7971..10742)
                     /gene="ppc"
                     /locus_tag="VF226_16610"
     CDS             complement(7971..10742)
                     /gene="ppc"
                     /locus_tag="VF226_16610"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEX7075661.1"
                     /translation="MTSNHRHADISQMDRSLREDIRILGRILGDTVRDQEGQSVFDVI
                     EHIRRTSIQFHRTEDEAAQRDLFETLNGLTQRNAKQVIRAYTYFLHLANIAEDHNQIS
                     RWRAEALAGSPQAEGTMAKALVDCAAAGCPHSEVCAFFERALVEPVLTAHPTEVRRKS
                     IIDNEMRIGQLLVQRDCMLATPEEEQRREEAFRRQILTLWYTSLLRDVPLSIRDEVLN
                     ALSYFDSTFLREIPRVYTALEDALAAAYPNCRISWLPSFLRIGSWIGGDRDGNPFATG
                     AVLREALRLQSNVAFRFYLEELHRLGSELSLDQRLAGVSEELRALAERSPDPSPHRRN
                     EPYRRAISGMYARLAATASDLNGFQAPYRPVGEAPPYDDASELRRDLDVLEQSLSEHR
                     SGLLAQGRLRDLRRAVDVFRFHLAGIDLRQHSAEHERTVAELFERARPGTGYSRLDED
                     QRVRLLLEQLEAAEPLVSSAQPYTPETTRELQILQAAAEAHRRYGPASVPNYVISMTS
                     GISDVLEVALLLAQVGLLRPLEPSLDVGIVPLFETIADLRNCSHIMDELLSLPAYRRL
                     VDSRGQSQEVMIGYSDSNKDGGFLTSAWELYKAEVALVDVFRRHKVHLRFFHGRGGSI
                     GRGGGPAYDAILAQPPGAVQGTIRLTEQGEVIASKYSNPEVGRRNLEILAAATLKATL
                     VPPDSGPPPPEYTDAMEQLSGEAFRAYRELVYETEGFERYFRESTVVDEISSLNIGSR
                     PASRKPSHRIEDLRAIPWVFSWAQCRLMLPGWYGFGGAVKAWLARHPKSGLQLLQSMN
                     GNWPFFRTLLSRLDMVLAKTDIAVASRYASLVDDARLRSLVFSKVRSELQDTTDALFA
                     ITGQKVLLESNPTLRLSLRQRVPYLDPLNHMQVELLRRYRSGDKSDSTVEAIHLTING
                     IAAGLRSSG"
     gene            complement(11217..13019)
                     /locus_tag="VF226_16615"
     CDS             complement(11217..13019)
                     /locus_tag="VF226_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX7075662.1"
                     /translation="MALAIEDYALIGDCRTAALVGRNGSIDWLCVPRFDSPACFAALL
                     GTSDHGHWRIGPKRPTSVSRRGYRGDTLILDTDFETETGIVRITDFMPMAEGNSSVVR
                     IVSGLEGQVELRMDLVIRFDYGRLVPWVTRGDGDLLAVGGSHQLVLRCPVEHHGENFT
                     TVAEFAVKAGEEIPFILSHGPSHLPAPAAFDARAALIDTEQYWTDWASKCRYDGPYRP
                     AVIRSLIVLKALTHHPTGGIVAAPTTSLPEEPGGVRNWDYRYSWLRDASMVLLTLLQA
                     GYREEAEAWRSWLTRAVAGLPQQLQPVYSVSGDRWLDEWEMPWLPGYENSRPVRVGNA
                     AFAQLQLDSFGEVLDALHHGRRSRLAPSAASWSLQKALLVYLETLRDVPDRGIWEIRA
                     PAQHFTHSKVMIWVAFDRAVSAVEHYGLDGPLERWRAIRDSLHAEICSRGYNSDVGAF
                     VQSYESRQLDASALMLPLVGFLPADDARIVGTVDAIGKHLMHNGFIRRYDAREPQDGL
                     PPGEGVFLACTFWFADNLILQGKWAEATDIFERLLEIRNDVGLLPEEYDPENGRFLGN
                     FPQALSHLALVGTAYNLREFHGPARQRSKLRRED"
BASE COUNT         2535 a         3995 c         4142 g         2501 t
ORIGIN      
        1 ggccgtgaag ctgtcagcat cgaaccaaac gtcttcgccc gcctcacctt ggccatagta
       61 gatttgcgga tcgatctgcc cgaaaagaaa atacgctgct gcaagggcaa atgctcccag
      121 ggtaaccgca atagaaatat cggaccggcg gcaatgcatt cgttaggaca gatgaagagt
      181 tgggcggacg aatgactatg acgaccgcct gtacggcagt cgcgtgagcg caggaaaagc
      241 gagcggcggc caactcaggc gcatcgaacc aggaagcgcc tactcttcga atgtcagcat
      301 ggtagttgat tgcgatctgc tccgccatgt cggaaggccg tttttcgaca gttaaaagga
      361 tcatctcttt gtttagtcga gaaaccggcc aacactccga tatgcgcaat gaacgtacca
      421 aagctggtgc tggccggcga aattggcctg cctagcggcg gttcgcagct attcacgttg
      481 cggtgatcag gcgtcttgct gaccggaaac ttcgtgcgct ttccggggtg gccggggcat
      541 tgactggatt gtcgcctggc cttattaact aaccagttgg tcactctgag ggcgcgtcgt
      601 gcgagcaggt tccgaggtgc gggtggctgt cgatgtcggc ggcacgttca ccgacattgc
      661 tttgatggcc gacaatggcg tgctgcatga gagcaagatc tcgacgacgc cggatgatcc
      721 cagcattgcg gtcgtcgatg ggctcatcgc gctgctcgat gagctgaaga tcgcaccctc
      781 cgccgtcgcc gaagtgctgc acggcacgac cgtcgggtcg aatacgatcc tgcagcgcaa
      841 gggcgcgcga accgggctca tcacgacacg cggattccgc gatgtgctgg agatcgggcg
      901 catccgcatg cccgatatgt tcgatctgac ctgggacaag cccaagccgc tggtgccccg
      961 ccggcaccgg ctggaggtga ccgaacggat cgcggccgac ggatcggtca ttgagcctct
     1021 ctcggagggg gacgtgatct ccgcagccga agcgcttctg gccgatggcg tagaggcgat
     1081 cgcgatctgc tttctcaaca gctatcgcaa tcccgcccac gagcagcagg ccgaggcgat
     1141 cctgaaggct cgcttccctg atctcgcagt gtcggcctca tacgccgtgc tgccggagat
     1201 gaaggagtac gagcgcacca gcacgacggt ggtgaacgcc tacttgctgg cggcgatgcg
     1261 ctcctacctc aggcggctcg agcagcggct cgcggcggcg ggcatcgcgg ctccgatact
     1321 ggtgatgacc tccaacggag ggatgctgcc tgccgccacc gctggcgaca agccggtgat
     1381 ggtggtggcc tcgggcccgg ctggcggtgt catcggcgcc gcccggctgg gggctgcact
     1441 gagcgagacc gacatcatcg tcttcgacat gggtggcacg accgccaagg ccgtcatcat
     1501 cgaggacggg cggccgagca tgacgtcgga gtacgagttc cgcgacggca tgtcgacgtc
     1561 gagccgcttc atcaaagcgg gcggctacat gctgaaggtg ccggcgatcg atattgccga
     1621 ggtcggcgcg ggtggcgggt caattgcggg gatcgacgcc gggggcttac tcaaggtcgg
     1681 cccggaatcg gccggcgcag tgcctggtcc agcctgctac agcctcggca acacgcgccc
     1741 gaccgtgacc gatgccaacg tcgttctcgg aatggtcaat ccgaaatcgc ttgcgggagg
     1801 gcgcctcgcg atcgaccggc gactggccga ggaggcgata cgggagcacg tggcgaggcc
     1861 gctcggattg tccctcgaag acgccgcgca tggcatccgg gctgtcgcca acgcggccat
     1921 ggcgcgcgcg atccgcgccg tgaccgtcga gcgcggccgc gatccgcggg acttcaccat
     1981 gatggcgttc ggcggcaacg gcggcaatca cggtgtcgac gtggcgcggc agctcggtgt
     2041 acgcaaaatt atcattcctc cgtttgccgg agtattcagc gccgtgggga tgctggccgc
     2101 cgatgtcgag catacggcgc ttgcgacagt gatgcggctc ctcgacacga tcacgccgac
     2161 cgagctcgga gagatcatgg acggactggc cgctgacgtt tctgctcggc tcgccgccga
     2221 aggctattct ggaaacaagg ccgagctcgc ctggcaggcc gaggtgcgcc acgagggcca
     2281 agcaaccgag ttgccagttc ctttcgaagc gaccggcgac aggtttgcgc aaatccgcga
     2341 gcggttcttc gccgagtact tcaagacata cggctaccgg gaccagacgc cgctcgagct
     2401 ggtcaaggtc accgtcgtcg ggcgcggcct gcgccgaaac cggctcgact tcagcaacat
     2461 gcggatcgat cagcggccgg gtgcggcgcg ttcgggccat cgcctcgtct cgttcacgcg
     2521 cggagagccc gccatcgaaa cggagatcgt gccgcgggcg gcggtgggat ctgagccgcg
     2581 gcgcgggccg ctgatcgtcg aggagttcga cgccacaatc gtggtgccgc ccgacaccag
     2641 catcagccgg gacaggacgg gcaacattgt tctcgagatc ggaggacagg catgaacgtc
     2701 gatccgatca cgtttgcggt catcaagagc ggccttgatt cgattgccga cgacatggcc
     2761 tacacggtcg tgcgcatcgc ccgctcggag atcgtcaagg atgtgatgga cttttccgcc
     2821 gccatctgcg ccgccgacgg acagatggtc gcgcaggcga agacgattgc acagcacctg
     2881 ggcgccatcc cccaagccat ggaggcggtc ctcgccaagt atggcgacga cctgcacgat
     2941 ggcgacgtcg tcatcatgaa cgacccctat catggcggca tgcacctgcc cgacatcttc
     3001 atgttcgtgc cgctgttcta cgagggccgt cgccgcgcct tcgcggtcgt gatctgccat
     3061 cacacggacg tcggcgggcg cgtgccgggc tccaatgcct ccgattcgac cgagatctac
     3121 caggaaggcc tgcgcctgcc gccgctgaag ctctatgagc ggggcaagct caacgagacg
     3181 ctcgaaactt tgatcaagat caacgttcgg gtgcccgatc gtgtctgggg cgatctctcg
     3241 gcccagtacg ctgcggccca ggtcggcaag cgcggactcg accgattgtt ccatcgctac
     3301 ggcgccgacg aggtcgaggc ctacatgcag gagctgctcg actatgcgga gcggctgacg
     3361 cgggccgaga tccggcagtg gccgaagggc acctatcgct tcacggacca catcgacaac
     3421 gatgggttct cggacaagcc gatccccatc agtgtcgcca tcacggtacg cgacgacggc
     3481 acgctgttgg tcgactacac ggggtcctcg ccgcaggtca aaggggcact gaactctacg
     3541 ctgagcttca cacattcgct cacgtatctc agcgtccgct gcgtgctcgg ccgcgacgtg
     3601 ccgaacaacg tcggtgtgtt ccgctgtctc gagatcaagg tgccggaggc atcggtgctg
     3661 aacccggtga tgccggggcc gtgtgcggcg cgcgcgctga ccggctaccg cgtgttcgac
     3721 accatgctgg gcgccctggc gcagatcgtg ccggaccggg tgcctgctgc cggtgagggg
     3781 ggcaacagcg tcatctgcat cagcgggctg aggccgaacc gcaagccatt catcatcgtc
     3841 gacatgatct gcggcgcctg gggcggacgg cccgacaagg acggtctcga agccgtcact
     3901 aacgcctcgc agaacctctc caacatgccc gtcgaagtca tggaggcgga gcatcccgtc
     3961 cgggtcgagg agtatgcctt cgtgcccgac agttgcgggg cagggcactg gcgcggcggc
     4021 atcgggctgc gccgctccta tcgcatcctg gcgcccgagg cgctgctgca aatgcggacg
     4081 gatcgcgtcg cgttccggcc ttacggcctc gccggcggcg agccgggcgg gccgtcgcgg
     4141 aactcgatcg agatcgatgg tgaggtgaag ccgttgcctg gcaaggttac catgaccgtg
     4201 ccgcaaggcg cggtgatcgt ccacgatcag gccggaggcg gcgggttcgg caatccgttt
     4261 acgcgcgatc ctgaattggt gctcgaggac gtcaaggacg gcaagatcac cgcggaattt
     4321 gctcgcgacc gacatggcgt ggtgatcacc gccggtcgcc tcgatctgga agcgacgcag
     4381 cgcctgcgtc aagaaagggt tgcatgacac gcggccgggc ccactccatg aatgccagtg
     4441 tagagctgca gccgaagcgg ccggtcaagg ccacgcgtag cgcggaggcc agccgcgcga
     4501 agatcctgga tgcggcccgc tacgagttcg tcacccatgg actcagtggg gcccgcgtcg
     4561 accggattgc cgaacgagcg ggcgtcaata agaacctgat ctatcattac ttcggcagca
     4621 aggacgacct ctacctccga attctggaag agatctacag cgacctcaga gcgagccagc
     4681 gtgatctgca ggtgcgcgac ctgccgccta tcgagggtat gcgctgcctc gtcggtcgca
     4741 ccttcgatca cttcgttgcc acgcccgcgc tcatccgctt gatgagcatc gagaacatcc
     4801 actatgcgcg ctatctgaag cgctccaagc tggtgaagcc gctttacacg cccttgctcg
     4861 acaccatcga acacctactg gccagagggc aggccgacgg tgttttccgg cgcgatgtcg
     4921 atgctgttga cctttatatt tccatctcgg gtctggccta cttctatctg tcgaaccggc
     4981 acaccctatc ctggatcttc gaccagcagt tcgattcacc gaagcgcctg gcacagcgcc
     5041 gtgcgcacat cgtggacatg gtgcttggat atctccagca taagggatca gtcgttgacg
     5101 aggcgatcgc ggcgtcgcga gaccaaacct cgcctcacgc gaaatcaact gcagaacggt
     5161 aaacaccgaa gatcagggag gatggaagat gagacggcgt gaacttctcg cattggcggc
     5221 ggcaggtgct atcgccttgg cctcgccagt cggggcgaat gcacaggaaa agctgacggt
     5281 ccggctcgat ttctcgccat ggggcgtgca ggccgggatg catcttgcca aggaaaaggg
     5341 ctggtttgcg gccgaagggc tcgacgtcga aatccaggac ggtcggggat cgggcaatac
     5401 cgtccagctc gtcaacgctg ggcaggtgga tgtcggtcag gtccaggtcg ggctcgtcgc
     5461 cagcgcgcgc gagcagggcg gcaaaatcaa gtcgatcttc aatttccagc gcaaaacaga
     5521 tctttgcatc ctggtcgatc gtgattcacc gatcaaaacc gtagccgacc tgaagggtaa
     5581 gaccgtcgtc gtattcgccg ccagcccgtg ggcgccgttc atcgatctct atctgaaatc
     5641 gggcggcatg gaccgggaaa ccgtaaaggt cgagttcgtc gatccggcgg cattgtgggg
     5701 cacctatacg gcaaggcgcg ctgatggtct catgtcgacg gtcggctcgg cgctgcccgt
     5761 cgcggagaag cctcggccct ccaaatgtct gctggcatcc gacgccggca tcggattccc
     5821 ggcttatggg ctcgtcgccc acgaggacac cattgcgtcg aagcgcgatg ccctcaagaa
     5881 gctgatcaag atccaacagc gggcctggga gcatctgcgc accaacatcg acgatggcgt
     5941 gaaggccatg attgcgcagc gtccggatgc aaagctcgat ccggaagtgc tcgctggtca
     6001 gatcaagctg acgatcgagt acttcgacac gccggccacc gcgggtaagc cgatcggttg
     6061 gcaggccaag gccgactggg aacaggcctt gaaatccatg gagcaggccg gcgtggtcaa
     6121 gccgggttgg aatgtcgacg actacttcac gaacgacctc atcgaatagc cgaaaatggc
     6181 tgccgctgca gaggcttatg tcaccatgtc gcgtgttggg aaggtctatc cttcccaaca
     6241 gggcgatgtc gtcgctttga ccgatttcga tctcgagatc cggcgaggtg agtttctgag
     6301 catcgtgggg ccgtcaggct gcggtaagag cacattgctg aagtgtgtgg cgggcctgga
     6361 gcctgtcagc gcaggaacga tggcggtcaa tggtcagccg gtacaggggc cgccagatca
     6421 gctcggcgtc gtcttccagc gcgacgtgtt gctcgattgg cgcacgatcc tcgacaatgt
     6481 gctgatctct gccgaattcc tcggccgcag gcgggccgat ctcaaggccc gggccatcga
     6541 gctgctgcag cgtttcgggt tgggtggctt cgaggaccgg cacccctggg agctctcggg
     6601 gggcatgcgt cagcgagcgg ccatctgccg tgcgctgctg tgcgatcccg agctgctcct
     6661 catggacgag ccgtttggcg cgctcgacgc catgacgcgc gacgatctca acgtcgagct
     6721 gacgcgcatt tggcaagata gcaggaagac ggtgctgttc atcacccata gcatcgtcga
     6781 agcggtctat ctttccgatc gcgtcgttat gatgtcgcgc gcgccggggc gtattgtcga
     6841 catcatcgac atcgacatcc cccgcccccg cccgctttct gtccgggagt cgaaacggtt
     6901 cggagagctg acgagcaaaa tccgtcacca tttcgcagag cttgggatcg taaaggaatg
     6961 aagcgcggca tcggtgactg gttcaaaaca gaaagtcgca cgcgcgatgt tttgcttgtg
     7021 ctggccgcat ccatcgccat ttgggagctg ggtgttcagg ttctggcgcc gtcgccgctg
     7081 atcctgcccg ctccatcagc catcgcccgc gagttcatga ccacgccggg cattttcatc
     7141 cgccatctgg gcttcacgct ggccgtaacg gtcgcgggtt tcctgctggc gctggtcatg
     7201 ggtgtggcgc tggccgtggg catcgtctac tcgcgctttc tggaacggac ggtctacacg
     7261 ctgctggtcg ccctcaacag cattccgaaa gtggcgctgg cgccgctgtt cgtcatctgg
     7321 cttggcaccg gcactgagcc gaagctggcc gtggccgtga tgctggcgat tttcccgatc
     7381 gtgatcgata cggtgctcgg cctgcgctct gtcgatcccg atatgctgaa cctcgcccgc
     7441 gcctcccgcg cttcggcttg gcagatcctg ttcaagatcc gctttccgtg tgccttgccc
     7501 agtctctttg ccggaatgaa ggttgctgtc tcgttcgcgc tggtcggcgc tatcgtcggc
     7561 gagttcgtag cgggcgggcg tggactgggc ttccagatcc tcgtcgcgca aggccagttc
     7621 gacaccccgc gcgtattcgt gtcattgctg ttgctgggta taatggggac gatcctgttc
     7681 tatgcggtcg attttgccga gcgtgtttgc ctgccctggc acgtttccca gcgaacgcag
     7741 cttgccaggc tcgaccattg agctcttggt gacttgcgca tcagtggagg cctccctggg
     7801 atagcgctcc cggcgaatca tgaatacctc tcttctcggc ttggacatat ccttccgcca
     7861 agtcgcggag cttgtctgga tttgacgcag tttcggcatc gccgggcgcg gttgcccaaa
     7921 ggtcgtctcg gtctttgcga aggcccagat tggaaatcga tctgacttgg tcagccgctg
     7981 ctcctgaggc ccgcggctat cccgttgatc gtcaggtgaa ttgcttcgac cgtgctatcg
     8041 cttttgtcac cgcttctata gcgccgcagc agctcgacct gcatgtggtt gagcggatcg
     8101 agataaggaa ctctttgccg aagagacagc ctcagcgtgg gattgctctc gaggagcacc
     8161 ttctgaccgg tgatggcgaa cagagcgtct gtggtatcct gcaattctga acgaactttt
     8221 gagaatacga gactgcgtag ccgagcatcg tcgaccagag aggcatagcg cgatgcgact
     8281 gcgatatcgg ttttcgcgag gaccatatcc aatctcgaca gcagcgtcct gaagaacggc
     8341 caattcccgt tcatcgactg gagcaactgg aggcccgatt tgggatgacg ggcaagccaa
     8401 gccttgacag cgccaccgaa tccataccag ccgggcagca tcagccggca ctgcgcccag
     8461 ctgaacaccc acgggatggc ccgcagatcc tcgatgcgat gcgaaggttt gcgagaggcg
     8521 ggccggctgc cgatgttcag gctggagatc tcgtccacca ccgtcgattc ccggaaatag
     8581 cgttcgaaac cttcggtttc gtagacaagc tctcgatagg cgcgaaaagc ctcaccagaa
     8641 agctgctcca tggcatccgt gtattccggt ggcggaggcc cgctgtccgg aggaactaat
     8701 gtcgccttca atgtcgcggc ggccagaatt tcaagattcc gtcgaccgac ttcgggattg
     8761 gagtacttgc ttgcgatcac ctctccctgc tccgtgaggc ggatcgtccc ctgaacggcc
     8821 ccggggggct gggcgagaat ggcatcgtat gctgggccgc cgccccggcc gatggagccg
     8881 cctcggccgt gaaaaaaacg aaggtgtacc ttgtggcgac ggaagacatc gacgagtgcg
     8941 acttcggcct tgtagagctc ccacgccgac gtcaaaaagc ccccgtcctt gttgctgtcc
     9001 gaatagccga tcatcacttc ctgggactgg ccgcggctgt cgaccagacg cctataggcg
     9061 ggcagggata gaagctcgtc catgatatgg ctgcagttgc gcagatccgc gatggtctcg
     9121 aacaggggca cgatcccgac atcgagagac ggctcgagcg ggcgcagcag accgacctgc
     9181 gcgagcagga gagcgacttc gagcacatcg gaaatgccgc tcgtcatcga aatgacgtag
     9241 ttgggcaccg aagccggacc gtaacgccta tgcgcttccg ccgcggcttg caatatttgc
     9301 agctccctcg ttgtttcggg cgtatagggc tgagcggacg atacaagtgg ctcggctgct
     9361 tcgagctgct ccaggagaag ccgaacacgc tgatcttcat cgagccgcga atagccggtg
     9421 ccggggcggg cccgctcgaa aagctcggcg accgtgcgct catgctcagc cgagtgctgg
     9481 cgcaaatcga tgccggcaag gtgaaagcga aacacatcga ccgcacgccg caggtcacgc
     9541 agccgcccct gtgcgagcag cccagaacga tgctccgaca acgactgctc gagaacatcg
     9601 agatctctgc ggagctcgct tgcgtcgtcg taaggcggtg cttctcccac gggacggtag
     9661 ggggcctgaa atccattgag gtccgatgcc gttgccgcca gcctggcata catgcccgat
     9721 atggctcgtc gatagggctc gttgcggcgg tgaggcgagg gatccggcga tctctcagcg
     9781 agggcccgca attcctcgga tacgcccgcg agccgttgat caagcgaaag ctcggatccg
     9841 agccgatgca gctcctcgag gtaaaagcgg aaggcaacat tgctctgcag tctcagagct
     9901 tctcgtaaaa ccgctccggt agcgaatgga ttgccgtccc ggtcgcctcc gatccagctg
     9961 cctattctca agaaagacgg gagccaactg atgcggcaat tgggatacgc tgccgcaagc
    10021 gcgtcctcca gagcagtgta gacgcgcggg atctctcgca ggaatgtgct gtcgaaatag
    10081 gaaagcgcgt ttaaaacctc gtcccggatc gaaagcggca catcccgcag caggctcgtg
    10141 taccacaagg tcaaaatctg cctgcggaag gcttcttcgc ggcgttgctc ctcctcgggt
    10201 gtcgccagca tacaatcgcg ttggaccagc agctgcccaa ttctcatttc gttatcgatt
    10261 atgcttttac ggcgaacttc cgtcgggtgt gccgtcagca ccggctcaac gagagcccgt
    10321 tcgaaaaagg cgcacacctc cgagtgcggg cagccagccg ccgcgcaatc cacgagcgcc
    10381 ttcgccatcg tcccctcggc ttgcggtgaa ccggcgagcg cctcggctcg ccaacggctg
    10441 atctggttat gatcctcggc gatattggcg aggtggagaa aataggtgta tgcacgaatg
    10501 acctgctttg cattccgttg cgtcaagcca ttcaacgttt cgaaaaggtc gcgctgtgcg
    10561 gcctcgtcct cggtgcgatg aaattggatt gaggttcgtc gaatgtgttc aatgacgtcg
    10621 aaaaccgact gcccttcctg atcacgcacc gtgtctccta gaatgcggcc aaggatacgg
    10681 atgtcttcac gcagtgaacg atccatttga gagatatcgg cgtggcgatg attcgatgtc
    10741 atggcccttc ggaggtaatc aatggcattg attgcacgtt atgcagtgct gcgaccaaca
    10801 actccgccat ctggcacgtg accggccccg cgccatgtct cgatttgtgc gcggcaggag
    10861 aagccatccg ctggacggct cagatcagcc gattgcattc ggtcgaacca gggtgcacgc
    10921 tgcatcgttc ccatccaact tgccgcaatg ggtcacaacg ggccggcacc acggcaatcg
    10981 tttctgtctg gcagaagcta gactccgcga ccgacgacaa tcgatctgcg gatgctggtc
    11041 cgctgctgct tgctcgacca gggcttgtaa cacgtcggca gcgtgcaagc ggcggctcgc
    11101 tctgggtatc caaatgcggc ctttaggatc taacgtgact gcacttcgcg caagcgagcg
    11161 ccctaccgtt cattgaaacc cgattggaac cgaggtcgat gggggagggc attcgttcaa
    11221 tcttcacgcc tgagcttgct gcgctggcgg gcaggaccgt ggaattcacg cagattgtag
    11281 gccgtgccaa ccagggcaag atgcgaaagc gcctgcggga agttaccgag aaagcgaccg
    11341 ttctccggat catactcttc cgggagaagt ccaacgtcgt tacggatttc gagtaggcgc
    11401 tcgaaaatgt cggtggcctc cgcccatttg ccttgaagga tgagattgtc cgcgaaccag
    11461 aaggtgcaag cgaggaacac gccctctcca ggcggcaatc cgtcttgcgg ctcgcgtgca
    11521 tcgtagcggc gaatgaagcc attatgcatc aaatgtttgc cgatcgcatc gaccgttccg
    11581 acgatccgcg cgtcgtcggc aggcagaaaa ccgacaagcg gaagcatgag agccgatgcg
    11641 tcgagctggc gggattcata cgattggacg aatgcgccca cgtcggaatt gtaccccctg
    11701 ctgcagattt cagcgtgcag tgaatcgcgt atcgcgcgcc agcgctcgag tggcccgtcc
    11761 aggccataat gctcaaccgc cgagacagcg cgatcgaaag caacccagat catcaccttc
    11821 gagtgagtga aatgctgggc cggtgcacgg atctcccata tgcctcgatc cggcacatct
    11881 ctgagagttt caaggtaaac gagtagagcc ttctgcaacg accagcttgc tgcgctcggg
    11941 gcaagtcgcg atcgacgacc gtggtgcaaa gcatcgagca cctcaccgaa ggaatcgagc
    12001 tgaagctgtg cgaaggcggc gttcccgacg cgtaccggcc gggaattctc gtagccgggc
    12061 agccagggca tttcccattc gtccaaccac cggtcgccag aaacgctgta cacgggctgc
    12121 aattgttgcg gcaggccggc aacagcacgc gtcagccacg atcgccaggc ctcggcctct
    12181 tcccgatatc cggcctgcag caaggtcaat agaaccatgc tggcgtcgcg tagccagcta
    12241 tagcgatagt cccaattgcg cactccgcct ggttcctcag gcaaggaggt ggtcggcgcg
    12301 gcgacaattc ctcctgtagg atgatgcgtc agtgccttga gcacgatcag agatcgaatg
    12361 actgccggac ggtatggtcc gtcatagcga cacttcgagg cccagtccgt ccagtactgc
    12421 tcagtgtcga tcaacgcggc cctggcatcg aaagcggccg gggcgggcag atgggatgga
    12481 ccgtgggaca agatgaatgg aatttcctcg cccgccttta cggcgaactc cgccaccgtc
    12541 gtgaaatttt caccatggtg ctctaccgga cacctcagaa cgagctgatg ggagccgccg
    12601 acggcgagga ggtcgccatc ccctctcgtg acccagggca cgagccgccc gtaatcgaag
    12661 cggatcacga gatccatgcg cagctcgacc tgtccctcga gccctgatac gatgcgcacg
    12721 acgctcgagt tgccctcggc catgggcatg aagtccgtaa tccgcacgat ccctgtttca
    12781 gtctcgaagt cagtatcgag gatcagcgtg tctccccgat agcctcggcg gctcacgctc
    12841 gttggacgtt tgggccctat ccgccaatgc ccgtgatcgg aggtgccaag cagagcggca
    12901 aaacaggcgg gagagtcgaa cctcggcacg cagagccagt cgatcgagcc attcctgcca
    12961 accagtgccg ccgtgcgaca gtcaccgatg agagcataat cctcaatggc tagggccatg
    13021 gctatcaccc ggatggtttc gaatactgca atttgctgcc gatcagaaaa caacgccaga
    13081 gccatctact gaactcacgg gggccggtga ggcgcggcgt cgcggagctc gagatggagc
    13141 ccaaaccttt cgaaccagtg gaaaggatcc tag
//
LOCUS       DASVDU010000254        11123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_775601, whole genome shotgun sequence.
ACCESSION   DASVDU010000254 DASVDU010000000
VERSION     DASVDU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775601"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF226_16620"
     CDS             <1..375
                     /locus_tag="VF226_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7075663.1"
                     /translation="DFILRQPQGYATLVGERGVNLSGGERQRLAIARALLKNPPILIL
                     DEATSALDAATEARIQKALDALMQNRTTFIIAHRLATVRGADQILVLNQGRLVERGSF
                     DELVAQNGLFANLVRTQFAGAH"
     gene            complement(786..1328)
                     /locus_tag="VF226_16625"
     CDS             complement(786..1328)
                     /locus_tag="VF226_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7075664.1"
                     /translation="MSPNTSLKLDALDHAIMKCLLKDARSSVAQIAKTLKMNESTIRH
                     RLNRLLENDVVDFALITNPLRFGFQVWSMLELQVEQSKIRTVAERIAKEPEVHLVGIM
                     SGSYDVYAGAVFRSNEDLVDFITHRLSRIPGIIRTSNSTMLEILKRTVSFGFPDEVMG
                     ATSSPLPIRKRRPAAKAKRK"
     gene            1650..2699
                     /locus_tag="VF226_16630"
     CDS             1650..2699
                     /locus_tag="VF226_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase"
                     /protein_id="HEX7075665.1"
                     /translation="MNPIDLVDLFRRELALCKVQGQKVAVLTGPRSRSDYAAALMAAG
                     QQLGAEVFRIDLPAQVNATDGVPTTAQGALWGRTPLTGHRVGVELLKMADIVVDLIGL
                     LHSPEQLEVQRAGTRVLMIVEPPDVLSRMFPRPNLRARVEAGGKRLAKADTLRVANPA
                     GTDITYKLGQYSRTPILQYGYTDEPGRWDHFPGGFVYTWPNEGEANGTIVLREGDIIF
                     PFKEYVRSNIHIDVKDGAIWSIRGGFDADHLKHFMDSWKDPDAYAMAHIGWGLDEMAL
                     WNAISLMNKETAIGQDGRAFYGNVLWSTGPNTDVGGSRDTPAHLDIAMRGADLFLDNE
                     PIVLAGEVVPEDMRA"
     gene            2759..3967
                     /locus_tag="VF226_16635"
     CDS             2759..3967
                     /locus_tag="VF226_16635"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX7075666.1"
                     /translation="MVFPVDRPLHKLKETHYLDNRIFTDQAIFEEEQRKIFEKVWCFV
                     CHESEVAEPGDYRAVTVGRHPLIIVRDRTRKLRALHNTCPHRGQRIVQKESGNVRSFQ
                     CFYHLWNFGLDGTLRQVTRPSGYENTGFELKDFCLPEVRVDSLCGLIFVTLDPEIEPL
                     ADFLGAAADHFRETLGTEPMEVFFFHRAIFQTNWKLWNDNNSELYHEYMHYLNRNTNL
                     KGDSFFNRSWRLYRNGHNAVEEAEVAYENGGLGNRADNRLPGTSPNGFKLINIFPDTV
                     VNCRSTIVRMDRMVPIGPGRTLIEWRGIGVKGEPESIRSMRERHFTEIWGPCGRNITE
                     DQIAVETQWLSMSAGSAPHTILAREEGMKPMDDGNLRAYYQEWGRRLGRKPNSPFDRN
                     PADAALVERA"
     gene            3998..4486
                     /locus_tag="VF226_16640"
     CDS             3998..4486
                     /locus_tag="VF226_16640"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7075667.1"
                     /translation="MLATAESATDANSDRFAIEAMIKTACWHLDTGEFDKWIGLFAAD
                     GVYEIEVYSPEIQKSMRWQRLTRMEMATLLSEMPNHLTEDARRAHMVMTVQANVDRDA
                     ASSISRFVVFKTNARGESGAYAIGTYVDDLVRQAGVWLISRRKVVLETRMFDVFPHII
                     PL"
     gene            4535..5659
                     /locus_tag="VF226_16645"
     CDS             4535..5659
                     /locus_tag="VF226_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075668.1"
                     /translation="MSSIVQKPLYPLGGLTRRSVLALTAAAAASQISPFNARAAAAVK
                     LGYLNINAMTSVYVPAANPWSDKTLDIQFSKFQGGPTIIQAIQSGGLHAGELAASVAI
                     SLASRGIPIVCLLTCNHATSTRPYNRMMVAKDSKIRSLGDLDGKTIGVLALGTLDHVE
                     VLTLLKKADIDPRRVKIVPVPVPNQAQALAAGQVDAMMMPPPADSVAEMQFGARMLAD
                     ATEGLPYYPVEVLVVDAAWAEANTETVRALVSGWLTVGRWIESNQAKARQAARTTLGI
                     SAELSDVMHLPRWCLNGLPVMPGMWNLYNTMVANKLVDPSPDPAAMMKRYFIDPAMKY
                     VIPALQEIGRVDDKETDSLKSIPLPYLTEDLRNYSAPWEV"
     gene            5702..6661
                     /locus_tag="VF226_16650"
     CDS             5702..6661
                     /locus_tag="VF226_16650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEX7075669.1"
                     /translation="MQGDSLRVKVSSVSDHGAEIKAYDIVAEQGGLLPQFTAGAHVDV
                     VLGDRLVRSYSICSSPDDRSHYRLCVARDANGRGGSIYFHENVRRGDSLTISPPKNLF
                     RLNESAEHSVLIAGGIGITPLLSMAHRLHVIGRSWEIHYAARSRTAAALLDEVEDFLK
                     AGISVNVYFSQDVDGCRLDMARVVNAAAASTHLYCCGPARMLTAFAQASAGRPRETIH
                     VERFSPQAAPATAGGFTVVLRKRNQSFYIPPGETILQVLRKNGVHVSSACEEGTCGSC
                     ETVVIGGRPDHRDSILNETERMSGETMMICCSGSLDEELILDL"
     gene            6669..7844
                     /locus_tag="VF226_16655"
     CDS             6669..7844
                     /locus_tag="VF226_16655"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX7075670.1"
                     /translation="MTPADVDAFRRMTACTPSWTALRPAHEAMDLSAATLLHAGPPFD
                     GAEQIPAPVRNSAIAAILYEGWAGSPAEAASLLQSGQVRLKPAQDCQCVLPLAAILSP
                     SMQVQIVADTAAGGKLSYSPLNGGSGPAMRLGLPTAAVVDHLRWINGPFSEAIRSAID
                     TGIDLLSIADTALLAGDECHGRTSEGTRAIHGELRKRASAGLPDAVEAFFASSPSFFL
                     NLWMAASKCILSAANGVPGSTMVTGAGGNGSRFGLIISGNPTDWITSPAMAPVGNRDP
                     GISTADCLPAIGDSAIVEALGLGAMALSYSPVQQAALHPFMPLPSETLSELLLIGGHP
                     RMARSRARVGLLAGQVRAVQQAPAVALGILDAAGRRGRVGGGIYVMPLELFDKACGL"
     gene            8053..9330
                     /locus_tag="VF226_16660"
     CDS             8053..9330
                     /locus_tag="VF226_16660"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7075671.1"
                     /translation="MRPKSPELLTAVEAIAAIEGGELSCEALIGACFDRIEAREADIR
                     AWVVHDRSRALKAARERDAGAIRLPLMGIPLAMKDIIDTADYPTSYGSAIYAAHRPRV
                     NASCVSILESQGAIVAGKTVSTEFAYFSPGPTANPRNRLHTPGGSSSGSAAAVADYMV
                     PIGFGSQTAGSLIRPAAYCGVIGFKPSYGRIALTGVKAFAPSLDTLGWMARDFDDVEL
                     VRASLLGKGFRKLAAVDGRIRVGFCPTPEWEMASAETQVALCRAADMLKRHAEVAETG
                     LSDAGLAQVQKTIMAFEAARALWHEYHHHKELLSRPLCDLIEAGLTTSYEDYLAAHRR
                     ARLASAEVEQLFSDCDVLMAPSAPGEAPVGLHNTGDPIFSRAWTLLGLPCITFPLPRD
                     KRKMPIGIQLIGALDGDRKLLEVAKSVAKWIET"
     gene            9340..10131
                     /locus_tag="VF226_16665"
     CDS             9340..10131
                     /locus_tag="VF226_16665"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075672.1"
                     /translation="MPPAGSIEIQNVSKRYAGPSDAQETSILDDISLSVHPGDFIGII
                     GPSGCGKSTLLSMIAGYTPMSSGQIQVGGRSVRRAGPDRVMVFQRPMLFPWLRVADNI
                     AFGLRLKAKREHMGDPQKIESEVGRLLELVNLTHCADRYPHQLSGGMQQRVEIARALA
                     VRPEVLLMDEPFGALDAFTRQEMQVEFVRIHQAARFTTIFVTHDVSEALLMADKVVVM
                     QVNPGRIADVVAVDVDRAQRKNSQRIREMEQHLSDLLSVTKGQSQ"
     gene            10152..10871
                     /locus_tag="VF226_16670"
     CDS             10152..10871
                     /locus_tag="VF226_16670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075673.1"
                     /translation="MLLVIWEAASRLGFVNQHILPAPSAIAATLFELIWSLELLHHLA
                     ASMYRIFTGFAIAVVSSVVLGLLMARFHLIEMVFDPLVELLRPVSPLAIFPVAILWFG
                     IGDASKIFLVALACSFPIILNTYAGVRAIDVNYIRAARSLGANGWEIIYKVIWVSCLP
                     HVFTGLRISWGISLIVIVAAEMIGSDSGIGYLILDAQQTFRIERVYAGVFVIGVVGFA
                     TDQIFRYLRRALLPWHREMRE"
BASE COUNT         2137 a         3233 c         3546 g         2207 t
ORIGIN      
        1 gatttcatcc tgcgccagcc gcagggctac gccaccctgg tgggcgagcg cggcgtcaat
       61 ttgagtggcg gggagcgcca gcggctggcg attgcccgtg cgcttcttaa aaatccgccg
      121 atcctgatcc tcgatgaggc gacctctgct ctggacgcgg cgaccgaagc ccgcatccag
      181 aaggcgctgg atgcgctgat gcagaaccgc accaccttca tcatcgcgca ccggctcgcc
      241 accgtgcgcg gcgccgatca gatcctggtg ctaaaccagg gccgtctggt cgagcgcggc
      301 agtttcgacg aactcgtcgc ccagaacggc ctgttcgcca atctggtgcg cacgcaattc
      361 gccggcgccc attgatgagc tttagcgggt ctattcaagt taagcccgga aatccccggc
      421 gcggtagagg gcggttgccc ggagcggcat gctgctggca cgggcctgcc cgttgaatgc
      481 ggaattgtgc ggctccgaat cgaacgggct gccgcggcag ccctcgccag atcaagcgac
      541 tctgtcgctg ctcggcgctc acaacagcag gcgcaggcca gaaagcgctg gcctctcggc
      601 gccccgatct gaccgccgac gggatgtttg cgcaaagccg ggaacgggcc tcaatgccac
      661 gaatgtctcg tcggcaggcg agctggaacc atgccagatt aggcgcggct tcccgtcggc
      721 cgccacccgc gggcgccatt ccatctgcag gtatgtggcg gatccgcttt cgaaacgagc
      781 agatgctatt tccgctttgc tttggcggcc ggccgccgct tccggatagg gaggggggaa
      841 ctcgtggcac ccatgacttc gtccggaaat ccgaaactca cggtgcgctt gaggatttca
      901 agcatcgtcg agtttgacgt ccttatgatc cccgggatcc gcgacaggcg atgcgtgatg
      961 aaatcgacga gatcctcgtt cgagcggaac acggcaccgg catagacatc gtatgatccc
     1021 gacatgatcc cgacgaggtg gacctcgggt tccttggcta tccgctccgc caccgtccgg
     1081 atcttcgatt gctcgacttg gagttcgagc atggaccaga cctgaaatcc gaatcgtagc
     1141 ggatttgtga tcagcgcaaa gtcgaccaca tcgttctcca acagcctgtt gaggcggtgc
     1201 cggattgtcg attcattcat tttcagggtt tttgcgatct gcgcaaccga agaccgcgca
     1261 tccttgagca ggcatttcat gatcgcgtga tcgagtgcgt cgagcttgag agaggtattg
     1321 ggagacatga gtcaacctag ccagtggggc ggattgtgaa gggaggccat gcaatcttgc
     1381 gcgtaaggcg cgcccatata atccaggttt gcacaaatat caccacggtt gcttccggaa
     1441 tcggcagaaa ataccggcat atgattaaga cttactctga tcggcttgtc gttcgtgagc
     1501 ggatgatcgg tatatttagc attaataatc ataatataca attataattg cggattccgc
     1561 aaaatataaa cggtattata gcgtttttcg taattgactc gcattacgaa agcagcaatg
     1621 ctggccgcga attgatattg aggtatccga tgaatcccat cgatcttgtc gacctgttcc
     1681 ggcgcgagct cgcactctgc aaggtgcagg gccagaaagt ggccgtcctc actggtcctc
     1741 gaagcaggtc cgattatgcc gccgccctca tggccgccgg ccagcaactg ggtgccgagg
     1801 tgtttcggat cgacctgccg gcccaggtga atgcgacgga cggcgtgccg acgacagcgc
     1861 agggggcgct atggggacgt acccccctta ccggccaccg cgtcggggtc gagttgctga
     1921 agatggccga tatcgtggtg gatctgatcg gcctgttgca ttcgccggaa cagcttgagg
     1981 ttcagcgtgc gggcacgaga gtgctgatga tcgtggagcc gcccgacgtc ctgagccgga
     2041 tgttcccgag gccgaacctg cgtgcgcgtg tcgaggcggg cggaaagcgg ctggctaagg
     2101 ccgataccct gcgggttgcc aaccctgccg gcaccgacat cacctacaag ctggggcagt
     2161 actcgagaac gcccatcctg caatacggct acaccgatga gcccggccgc tgggaccatt
     2221 tccccggcgg gttcgtctat acatggccga acgaggggga agccaacggc accatcgtgc
     2281 tgcgcgaggg cgatatcatc tttcccttca aggaatatgt ccgcagcaac atccatatcg
     2341 acgtgaagga tggcgccata tggagcatcc gcggcggctt tgatgccgat cacctgaagc
     2401 atttcatgga tagctggaag gacccggatg cctatgccat ggcgcatatc ggctggggcc
     2461 tcgacgagat ggcgctttgg aatgcgatct ccctgatgaa caaggaaacc gccatcggtc
     2521 aggacggcag ggccttctac ggcaacgtcc tttggagcac gggcccgaat acggatgtcg
     2581 gaggctcccg cgacacgccg gcccatctgg acatcgccat gcgcggtgcg gacctgttcc
     2641 tcgacaacga gccgatcgtc ctggccggcg aggtggtgcc ggaagacatg cgtgcatgag
     2701 gccgccagga aaaggctcgg tccgcgcaga gttggaaaga ctgtaggcta ggagaagaat
     2761 ggtgtttccc gtagatcgtc cgctgcacaa gctgaaggaa acgcattatc tggacaaccg
     2821 tatctttacg gaccaggcga tcttcgaaga agaacagcgc aagatattcg aaaaggtatg
     2881 gtgcttcgtc tgtcatgaaa gcgaagtggc cgaacctggc gactaccggg cggtcacggt
     2941 cggaaggcat cccctcatca ttgtcaggga ccggacgcgg aagctgcgcg ctctgcataa
     3001 cacctgcccg catcgggggc agaggatcgt ccagaaggaa tcgggcaatg tcagatcctt
     3061 ccagtgtttc tatcatcttt ggaatttcgg gctggatggc acgctgcgtc aggtgacccg
     3121 tccatcgggc tacgagaata ccgggttcga gctcaaggat ttctgcctgc ccgaggtcag
     3181 ggtggatagc ctctgcggcc tcatcttcgt gacgctggat ccggagatcg agccgctcgc
     3241 agacttcctg ggggccgccg cggatcattt ccgggagacc ctcggtaccg agccgatgga
     3301 ggtgttcttc ttccatcgcg cgatcttcca gacgaactgg aaactctgga acgacaacaa
     3361 ttccgagctc tatcacgaat acatgcacta cctgaaccgc aacacgaatc tgaaggggga
     3421 ttccttcttc aaccggtcgt ggcggcttta tcgcaacggc cataacgcgg tcgaagaggc
     3481 ggaggtcgcc tacgagaatg gcggcctcgg taaccgggcc gacaataggc tgcccggcac
     3541 cagtccgaac gggttcaagc tgataaacat ctttccggac acggtcgtca attgcaggtc
     3601 gacgatcgtc cggatggacc ggatggtgcc gatcggtccc ggaaggacac tcatcgagtg
     3661 gcgcggcatc ggggtgaagg gagagccgga aagcatccgc tcgatgcgcg aacgccactt
     3721 cacggagatt tggggcccct gcggcagaaa tatcaccgag gatcagatcg cggtggaaac
     3781 ccagtggctt tcgatgtctg cgggatccgc gccccacacg atattggctc gtgaggaggg
     3841 catgaagccg atggatgatg gaaatctacg ggcctattac caggaatggg gccgtcggct
     3901 cggccgcaag ccgaattccc ccttcgatcg caatcccgcc gacgcggctc ttgtcgagcg
     3961 ggcgtaggtc cggcgcgtgc acggagagga tctgcatatg cttgctacag ccgaatcggc
     4021 gacagacgcg aattccgacc ggtttgcaat cgaggcgatg atcaagaccg cctgctggca
     4081 tcttgacacg ggggaattcg acaaatggat cgggttgttc gcagccgacg gggtctatga
     4141 aatcgaggta tacagtccgg aaatacaaaa gagcatgcgg tggcagcgcc ttacgcgcat
     4201 ggagatggcg acgctgctct cggagatgcc caatcacctc accgaagacg cgcggcgcgc
     4261 ccacatggtc atgaccgtac aggccaatgt cgatcgcgac gcggcgagtt cgatttcgcg
     4321 cttcgtcgta ttcaagacaa atgcacgcgg cgagagcggc gcatacgcga tcgggacgta
     4381 tgttgacgat ctggtgaggc aggccggggt atggctgatc agccgacgca aggtcgtatt
     4441 ggaaacgcgc atgttcgacg tctttccgca catcatcccc ctataggggg cggcgggcgc
     4501 aactcgtcaa tatctgcaga tcggagatca tagcatgtcc agcatcgttc agaagccctt
     4561 atacccgctt ggcggcctga cacgccggtc agtgttggcg ctgaccgctg cggcggcggc
     4621 cagccagatc agccccttca atgcacgcgc ggccgcggcc gtgaagctcg gctatctgaa
     4681 tatcaacgcg atgacatccg tctatgtgcc ggccgcaaat ccttggtcgg ataagacgct
     4741 cgacatccaa ttctcgaaat ttcaaggcgg gccgacgatc atacaggcca ttcagagcgg
     4801 cggcctgcat gccggcgaac tggccgcatc ggtcgccatc agcctggcga gccgcggcat
     4861 cccgatcgtc tgcctcctga catgcaacca tgccacctcg accaggccct acaaccggat
     4921 gatggttgcc aaggattcca agatccggtc gctgggcgac ctggacggaa agacgatcgg
     4981 cgtgctggcg ctcgggacac tggatcatgt ggaggtcctg acgctgctga aaaaggcgga
     5041 tatcgatccg cgccgggtca aaatcgtgcc cgtgccggtg cccaatcagg cgcaggcgct
     5101 ggcagccgga caggtggacg ccatgatgat gccgccgccg gcggacagcg ttgcggagat
     5161 gcagttcggc gcgcgcatgc tggccgatgc gacggagggg ctgccctatt accctgtgga
     5221 ggtgctcgtg gtcgacgccg cctgggcgga ggcgaatacc gagaccgtgc gggcgctggt
     5281 ctccggctgg cttacggttg gccggtggat cgagtcgaac caggccaagg caaggcaggc
     5341 cgccaggacg acgctgggca tctcggccga gttgagcgat gtcatgcatc tgccgcgttg
     5401 gtgcctgaat ggactgcccg tcatgccggg tatgtggaac ctctacaaca ccatggtggc
     5461 gaacaagctc gtggatccct ctcccgaccc ggcggctatg atgaagcggt acttcatcga
     5521 cccggccatg aaatacgtca ttccggcgtt gcaggagatt ggccgggtgg acgacaagga
     5581 gacggacagc ctgaaatcca ttcccttgcc ctacctgacg gaggatctcc gaaactattc
     5641 ggcgccttgg gaggtgtagt cggatcccct gcgtctaggc tgcgtatcgg gttggggtgg
     5701 aatgcaaggt gacagtcttc gggtcaaggt aagctccgtt tccgatcatg gggcggagat
     5761 caaggcgtac gatattgtcg ccgagcaggg tgggttgctt ccgcaattca cagccggggc
     5821 acatgtcgac gtcgtcctgg gcgatcggct ggtgcggtcc tattcgatct gcagcagccc
     5881 ggacgatcgc agccattacc ggttgtgtgt tgcccgagat gcgaatggcc gcggcggctc
     5941 gatttacttc cacgagaatg tccggcgcgg cgacagtctg acgatttccc cgccgaagaa
     6001 cctctttcgc ctgaacgaga gcgccgagca cagcgttctg atcgccggcg gaatcgggat
     6061 aacgcctctt ttgagcatgg cacaccggtt gcatgtcatc ggccgatcat gggagatcca
     6121 ctatgcggct cggtcgcgca cagccgccgc cttgctcgac gaggtggagg attttttgaa
     6181 agcaggcatc agcgtcaatg tgtacttcag ccaggatgtc gatggatgcc gcctggacat
     6241 ggcgcgagtc gtcaatgccg ccgctgcgag tacgcatctt tactgctgtg gtccggcaag
     6301 aatgctgacg gcgtttgcac aggcgtccgc aggccgcccc cgggaaacga tccatgtgga
     6361 gcggttttcg ccccaggcgg ctcccgccac tgcaggcggt ttcacggtcg tcctgcggaa
     6421 gcggaaccag tccttttaca tcccgccggg agaaacgata ttgcaggtgc tccgcaagaa
     6481 tggagtgcac gtttcttctg cctgcgaaga ggggacctgc ggttcctgcg agacggtcgt
     6541 catcggcggc cggccagacc atcgcgacag catcctgaac gagacggaaa ggatgagcgg
     6601 cgagacaatg atgatctgct gttccggctc cttggatgag gaattgatcc ttgacctgtg
     6661 agcaacgcat gacgccggcc gacgttgacg cattccggcg gatgacggcc tgcacgccgt
     6721 cctggacggc attgcggccg gcgcacgaag ccatggatct ttctgccgcg acattgctgc
     6781 atgccgggcc gccgtttgac ggcgccgagc aaatcccggc tccggtgagg aattcggcga
     6841 tcgccgccat cctgtacgag ggatgggccg gcagcccggc agaggccgca tctctcctgc
     6901 agagcgggca agttcgtctg aagccggcgc aagattgcca atgcgtcctc ccgctggccg
     6961 ccattctgtc gccgtcgatg caggtgcaga tcgtcgcgga tacggcggcc ggcggcaaat
     7021 tgagctactc gccgctgaac ggcggcagcg ggccggcgat gcggctgggc ctgccgacgg
     7081 cagcggtcgt ggaccatttg cgctggatca acggtccgtt ttcagaggcg atccgatcgg
     7141 cgatcgacac tggcatcgat cttctgtcga ttgccgatac ggccctgctg gccggcgatg
     7201 agtgccatgg gcgcacctcg gaaggaacac gggcgatcca tggcgagctt cgcaagaggg
     7261 cgtcggcagg cttaccggac gctgtcgagg ctttcttcgc ctcttcacca agtttcttcc
     7321 tgaatctatg gatggctgca agcaagtgca tcttgtctgc agcgaacggt gtcccgggaa
     7381 gcaccatggt gacgggcgcg ggcggaaatg gcagccgctt cggattgatc atatccggca
     7441 atccgacgga ctggatcacg tcgccggcca tggccccggt cggaaaccgg gatcccggca
     7501 tctccactgc cgattgcctt ccggccattg gcgacagcgc gatagtcgaa gccctgggcc
     7561 tcggcgccat ggcgctgtcc tattcaccgg tacagcaggc ggccctccat ccattcatgc
     7621 ctttgccgag cgaaacgctg tccgagcttc tgctgatcgg cggccacccg aggatggcga
     7681 gatcgcgtgc ccgggtcggc ctgctcgccg gacaggtccg ggccgtccag caggcaccgg
     7741 cggttgcgct gggcatcctc gatgcggccg gacgtcgcgg acgcgtcgga gggggcatat
     7801 acgtcatgcc gctggaactc ttcgacaagg cctgcggtct gtgagggaga tggaggaggg
     7861 gaatgggggt atgcggccag atctgccagg cttggaaggg cggcgaaacg ttgcccatgc
     7921 tgttgccgcc acgcgtccgg tctccgggat cgatggcagg tggggtatcg cccatttgcg
     7981 gctgccggtc tttgggatgc aggcgccccg cgtcgttgcg gctcgggttg ggtgcagatc
     8041 ggggcgcgtg aggtgaggcc aaaatcgccc gagcttttga cggccgtgga ggccatcgcc
     8101 gcgatcgagg ggggcgagct gtcatgcgag gccctgatcg gggcatgctt cgatcgcatt
     8161 gaagcgcgcg aggccgacat acgggcgtgg gtcgttcatg atcgcagtcg ggccctcaag
     8221 gcggcgcggg aaagggatgc cggcgcgata cgtctgccgc tgatgggaat tccgctggcg
     8281 atgaaggaca tcatcgacac ggcggattac ccgaccagct atggatctgc catatatgcc
     8341 gcacatcgcc cccgagtgaa cgcctcctgt gtttcgattc tcgaatcgca gggcgcgatc
     8401 gtcgcgggca agacggtcag caccgaattc gcctatttca gccccggacc gacagccaac
     8461 ccgcgcaaca ggctccatac gccgggcgga tcatcgagcg gttcggctgc cgctgtcgcg
     8521 gattatatgg tgccgatcgg cttcggctct cagaccgccg gatcgctcat ccgcccggcc
     8581 gcgtattgcg gcgtgatcgg attcaagccg agctacggca ggatcgccct taccggggtg
     8641 aaggcgtttg cgccgagcct ggacaccttg ggatggatgg cccgcgattt cgatgatgtc
     8701 gagctggtcc gagcatcgct tctagggaaa ggcttccgca agcttgcggc ggtagacggc
     8761 aggatccgcg tgggattctg ccctaccccg gaatgggaga tggcctcggc agaaacccag
     8821 gtggcacttt gtcgtgcggc ggacatgctc aagcggcatg ccgaggtggc cgagacaggt
     8881 ttatccgatg caggacttgc gcaggtacag aagaccataa tggcctttga ggccgctcgc
     8941 gcgctgtggc acgaatacca ccaccacaag gaattgctca gcaggccgct ttgcgacctg
     9001 atcgaggctg ggctgaccac ctcctatgag gattatctcg ccgctcaccg gagggcgcgc
     9061 ttggcaagcg ccgaagtcga gcagctattc tccgattgcg atgttctgat ggcgccgagt
     9121 gcgccgggcg aggcgccggt cggattgcac aataccggtg atccgatttt cagccgagcc
     9181 tggacccttc tgggtttgcc atgcatcaca ttcccgttgc cccgtgacaa gcggaagatg
     9241 cccattggaa tccagcttat tggagcattg gatggcgaca ggaagcttct ggaggtggcg
     9301 aagtcggtcg caaaatggat tgaaacctag gagtttccaa tgccccccgc tggttcgatc
     9361 gaaatacaga atgtctcaaa gcgctatgcc gggccaagtg atgcgcagga gacgtccatc
     9421 ttggacgata tatcgctctc ggtccatccc ggggatttca tcgggatcat cgggccgagc
     9481 ggctgcggga agagcacatt gctgtccatg atcgccggct acaccccgat gagcagcgga
     9541 cagatccaag tcggtggccg atcggtgcgc cgggccggtc ctgaccgggt catggttttc
     9601 cagcggccga tgctgtttcc ctggctgcgt gtggcggaca atattgcctt tggcctgagg
     9661 cttaaagcca agcgcgagca tatgggcgat ccgcagaaga tagagagtga agtgggccgc
     9721 cttctcgaac tcgtgaacct tacgcattgc gccgacaggt atccgcatca gttatcaggc
     9781 ggcatgcagc agcgcgttga aatcgccagg gcgctggcgg tccggccgga agtacttctc
     9841 atggatgagc ctttcggagc gctggacgct ttcacccggc aggaaatgca ggtcgagttc
     9901 gtccgcatcc accaggcggc aaggtttact acaattttcg taacgcacga tgtctctgag
     9961 gcattgctga tggccgacaa ggtcgtcgtg atgcaggtca atccgggccg catagccgat
    10021 gtcgtcgcgg tcgacgtgga ccgggcgcaa cgcaagaata gccagcggat tcgcgaaatg
    10081 gagcagcatc tttccgatct gttgagcgtg acaaaaggac aatcgcaatg aagcggctcg
    10141 tatcaccggt attgctgctg gtcatctggg aggccgcttc gaggctcggc ttcgtcaatc
    10201 aacatatcct tcccgcccca tcggctatcg cggcgacttt gttcgagctc atatggagcc
    10261 ttgagctgtt gcaccatctt gcagcgagca tgtaccggat tttcaccgga tttgccattg
    10321 ctgtcgtcag ctccgtcgtg cttgggctat tgatggctcg gtttcatctg atcgaaatgg
    10381 tctttgatcc tcttgtcgaa ttgctgcgac cggtttcgcc gctggcgatc tttcccgttg
    10441 cgatcctatg gttcggcatc ggagatgctt caaagatttt cctcgtcgcc ctggcctgtt
    10501 cctttccgat catcctgaac acctacgccg gagtgcgcgc gatcgacgtg aactatatcc
    10561 gggcggcgcg gtcgctcggc gcgaatggct gggaaatcat ttacaaggtg atttgggtaa
    10621 gctgcctgcc ccatgtcttt accggactgc gaatttcttg gggcatctcg ttgatcgtca
    10681 tcgtggctgc ggagatgatc ggcagcgatt ccggtatcgg ataccttatt ctcgatgcgc
    10741 agcagacctt ccggatcgaa cgcgtgtatg ccggcgtgtt cgtgattggc gtcgttggtt
    10801 ttgcaacgga tcaaattttc aggtatctgc ggcgtgcgct gctgccctgg caccgggaga
    10861 tgcgtgaata gcatcaggtg cgcctgcgcc ctccgatcgg catcgcacga ttttgacgga
    10921 aattccggcg attgccgttg aagccctatt tccgggccgc ggctatcggg ctgcatccgg
    10981 accgacggca ccgatatccg cgatgtcacg ctggagagcc tgcgcagcca tatcggcgtc
    11041 gtcttccagg acagcgccgt cttccaccgc agcatcgccg acaatatccg catcggccgg
    11101 ccggaggcga caaatgccga ggt
//
LOCUS       DASVDU010000255        42023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1604214, whole genome shotgun sequence.
ACCESSION   DASVDU010000255 DASVDU010000000
VERSION     DASVDU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 42023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42023
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604214"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VF226_16675"
     CDS             complement(<1..747)
                     /locus_tag="VF226_16675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7075674.1"
                     /translation="MPPYLTPLLVVLVPLVGMSALHLFLPVVAPEMMAAAGVDPTAYG
                     WIAGAAGIGSIWLYMSNHGITPALGPVRTLRVGILIGLAGAALAMTAYLPLMVIGAVL
                     LGFGYSTSTPAGSQILADQTPKANWSMLFSLRQAGVPLGGVVSGGLGGLLIAQFGWRL
                     AFALIGLICLTLALVLLFVPRSYNTTRPLKAFRLSAIFSPSNLQRPFRALGLAPGLIR
                     LALACAGFATVQSAFFSFYVIYLHAGLGYGL"
     gene            complement(781..918)
                     /locus_tag="VF226_16680"
     CDS             complement(781..918)
                     /locus_tag="VF226_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075675.1"
                     /translation="MAVLPSSVTNLGIKDRVAAILAYGEMTGAEKGTSCACPIASELK
                     D"
     gene            1034..1993
                     /locus_tag="VF226_16685"
     CDS             1034..1993
                     /locus_tag="VF226_16685"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX7075676.1"
                     /translation="MTSTAPIAEATSFPHRHLLTCETLTAAEITALLDLADNAAEINR
                     QVNKKRDVLRGRTLINLFFEASTRTQSSFELAGKRLGADVMNMNVSTSSVQKGETLID
                     TAMTLNAMRPDIITVRHYAAGAVELLAQKVDCSVINAGDGAHQHPTQALLDALTIRRA
                     KGRIAGLTVAICGDILHSRVARSNLNVLNTMGAHVRLIAPSTLLPAEPERFGAEVFTD
                     MWKGLEGADIIMMLRLQRERMHGSLVPSSREYFHFFGLDHEKLARAKPDALIMHPGPM
                     NRGVEIASTIADHSRSLIREQVEMGVAVRMAVLEALARNLPNR"
     gene            2002..3336
                     /locus_tag="VF226_16690"
     CDS             2002..3336
                     /locus_tag="VF226_16690"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7075677.1"
                     /translation="MRSANSKIAATTRRTVFINARLIDPASNRDEPGGLLVADGEIAD
                     LGGHLRRSAPEGADVIDCKGHVLAPGLIDMQVFIGEPGAEHRETLKTASQAAAAGGVT
                     TIVVMPDTNPVIDQVALVDFIQRRARDNAVVNVHTMAALTKGLEGQEITEIGLLKRAG
                     AIAFSNGKNTIANTRVMRNALLYGKDFNTLIVHHTEDPYLTEGTCMNAGPVAARLGLL
                     GAPKAAEAIVLERDVRLVEITGGRYHAATISCRESLDILRAAKARGLPVTCGASINHL
                     TLNENDIGSYRTFFKLRPPLRAEEDRAALVRGLAAGDIDVIVSSHDPQDADVKRRPFA
                     EAADGAIGLETLLSAALRLHYTDGVSLMGLLRAMTINPARLLGLPSGRLEKGAPADLV
                     LFDMAQPWVVDREALRSRSKNSPFDETKMQGRILLTMVEGETVYRYPESQRG"
     gene            3375..3974
                     /gene="plsY"
                     /locus_tag="VF226_16695"
     CDS             3375..3974
                     /gene="plsY"
                     /locus_tag="VF226_16695"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7075678.1"
                     /translation="MDIAPATLVLAIAIGYALGSIPFGLLLTRLAGLGDIRHIGSGNI
                     GATNVLRTGNKSLAAATLVLDAVKGTAGVLVGSALGGPLAALAGGLGAFLGHIFPVWL
                     RFKGGKGVATYIGILLALSWPACLVFCAIWLAVAVLSRYSSLSALVASIVAPLVLWLL
                     GEGQPAALVGVLSVLLIIKHHANIQRLIAGTESKIGARA"
     gene            3961..5145
                     /gene="dprA"
                     /locus_tag="VF226_16700"
     CDS             3961..5145
                     /gene="dprA"
                     /locus_tag="VF226_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX7075679.1"
                     /translation="MPGPDDKPRPKPPPLPTARLDDSQRLACLRLIRSENVGPATFRA
                     LINHFGGARAALEALPELSARGGRRTGIRICSQESAERELAAAERIGARPLFTIEPGY
                     PAALAVIDGAPPLIYVKGDDRLLARPAVGIVGSRASSAAGQQLARQLAAGLGQAGYVV
                     ASGLAIGIDAAAHQASLETGTIAVLAGGIDVVYPPQNAALHHAIGETGCLVSEQPPGF
                     QPRGKDFPRRNRIISGLSLGVVVVEAARRSGSLITARRAADQGREVFAVPGHPLDPRA
                     AGTNQLIKEGATLVTCAEDVVNALEPFGRRPYPMEQARTFEESASAPNATPEPLPDIA
                     HDERSLVVAALGPSPVHVDELARATGLPIRIVQTVLLELALAGRIEHHGSQLVSLIAG
                     RD"
     gene            complement(5307..5579)
                     /locus_tag="VF226_16705"
     CDS             complement(5307..5579)
                     /locus_tag="VF226_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075680.1"
                     /translation="MGVIVMGQARDGKCRLALVRGEREALQPAQTEPGPTREQFEYLA
                     DMIRHMQVLAWQTGCSRVAELLELAHREADQHRRALPRRLSWIGPG"
     gene            5800..8523
                     /gene="topA"
                     /locus_tag="VF226_16710"
     CDS             5800..8523
                     /gene="topA"
                     /locus_tag="VF226_16710"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX7075681.1"
                     /translation="MNIVIVESAAKAKTINKYLGSNYKVIPSFGHVRDLPSKDGSVEP
                     DRDFAMHWDVDPKAAKVMREIADAVKKADKLILATDPDREGEAISWHILQLLDQKRAL
                     KKGVPVERVTFNAVTRDAVLAAMKNPRQIDAPLVEAYLARRALDYLVGFTLSPVLWRK
                     LPGARSAGRVQSVALRLVCDREAEIEAFKSEEYWTIEALLATPKGEEVRARLVSISGR
                     LLKKLDIKDQGSAHAIKAAIEGGRFTVSSVEKKAVRRNPAAPFTTSTLQQEASRKLGF
                     SAKQTMQVAQRLYEGVDVGGETVGLITYMRTDGVQIVPEAVSEIRRVIEGEYGKRYTP
                     FAREYKSKAKNAQEAHEAIRPTDPSRRPRDVARYLEKDQAALYELIWCRTIASQMAAA
                     ELEQTTAEITVEGKDGKTYGLRATGSVVVFDGFMRLYSEGRDDRVSSKDQVDLEDEDD
                     SRRLPPLERGDVLKDKAVTATQHFTEPPPRYTEATLVKRMEELGIGRPSTYATTLAVL
                     RERGYVRLDKKRLIPEDKGRLVTAFLESFFRRYVEFDFTADLEEKLDLISDGKLEWKD
                     VLRDFWRDFTGAVDEIKDLRVSEVLEALNEILGPHIFPPRADGVDPRTCPVCGSGRLS
                     LKVGRFGAFIGCSNYPECRYTRQFTEPQGEAEATPPEGKLLGYDPDTGLPVSLRTGRF
                     GPYVQLGEAEDGEKPKRASIPRGLDAATIDLERALQLLALPREVGLHPETGKPITAGI
                     GRYGPFVLHEGTYANLESLEDVFTIGINRAVALLAEKAAGGKGKWQRPKAQVLKDLGE
                     HPEGGKVEVLSGRYGPYVKHGSVNATLPKGKDPAEVTLEEAVELLAARAANGGAKRGK
                     TKAPSKNAAAGKAGSEKPSANKTSAPAKKAPAKKSAKPRSTKTRVPEPAAE"
     gene            8652..10937
                     /gene="rnr"
                     /locus_tag="VF226_16715"
     CDS             8652..10937
                     /gene="rnr"
                     /locus_tag="VF226_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX7075682.1"
                     /translation="MARKRTAKGEVKTKTAGGLPTKGEILDFIKGAQEKVGKREIARA
                     FGIKGSARIELNRLLAELSREGAITGLRKEVRARGALPPVSVLEIVGRDEDGDLFAEP
                     VVWDRTEGVRPKVLLIKSRRFLDAGEGGLGIGDRILARVRQLEGADIEGFRFEGEPIK
                     KLPREKRRLLGVYRANKKDGGGLIAPIDRKQLKDWLVQPGDEGEAKDGDLVRFDLPRV
                     FRYGIPQARVLESLGNPQDQRQISLIAVHAHGIPDDFPEGVLAELNRLEPPRADGRVD
                     LRAVPLVTIDPKDARDHDDAVHAEPDPDPKNRGGFIVTVAIADVAHYVRPGTRLDREA
                     QIRGNSVYFPDRVVPMLPERISNDLCSLREHEERPCLAVRMVFDSRGEKRRHTFVRGI
                     MRSAAKLSYEEAQAAFDGKPSRKCAALMGTVLEPLWAAYQVLAAARARREPLDLDLPE
                     RKIVLDAEGRVERVVIPERLEAHRLIEEFMIQANVAAAETLEQKRTALIYRIHAPPSK
                     EKVTALRTFLDTLQLRLPPGELKPAHFNRVLAQAKALPTADLINEVVLRAQSQAEYSI
                     DNIGHFGLNLRRYAHFTSPIRRYADLVVHRALVRALHFGPDGLTDEEAERLAEVAQSI
                     SEAERRAMAAERETIDRLIAAHLADRVGAEFEARISGVTRSGLFVRLKDTGADGFVPV
                     SSMIGDFYHLVEEQHALVGSRSQEAYRLGDEVRVRLVEAIPTAGALRFEMLSKGKRGR
                     VLLTKGWRGARTIRGRRRAAL"
     gene            11054..11485
                     /locus_tag="VF226_16720"
     CDS             11054..11485
                     /locus_tag="VF226_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX7075683.1"
                     /translation="MTVTIGRKRNLYDAILRGARHKCPACGIGRLFRAYLKVADTCPH
                     CHEELYHHRADDAPPYLTIVIVGHVIVAGILALEQTVAPPQWLQLALWLPLTVVMSLV
                     LLPTIKGSVVGLQWANEMHGFGTHPDPDEIEHEVARSPSST"
     gene            11506..12312
                     /locus_tag="VF226_16725"
     CDS             11506..12312
                     /locus_tag="VF226_16725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7075684.1"
                     /translation="MTTPSTPPTGTDPDKSMRPRDAATLVIVDRVLGVPRVLMGQRRF
                     DQVFMPGKYVFPGGRVDESDRAVESADELHPVEVSKLLLDMKGHPSPVRARALGMAAL
                     RETFEEAGVVIGAPASSSAETRCEAPSWNAFFACGYLPKLSSLRLFARAITPPGRPRR
                     YDTRFFHISADEISHQVDTSDGELLGLHWLTIEDARSLDLPSITRVILEDLTDFLRDG
                     ASGSGDAPVPYYHHKNGIFRRDLLSVADASMQLDTEGGQGMVRPISNERA"
     gene            12333..12500
                     /gene="rpmG"
                     /locus_tag="VF226_16730"
     CDS             12333..12500
                     /gene="rpmG"
                     /locus_tag="VF226_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7075685.1"
                     /translation="MAKPITQKIKLLSTAGTGYFYVTKKNPRTSTEKLSFKKYDPVVR
                     KHVEFKEAKIK"
     gene            complement(13128..14501)
                     /locus_tag="VF226_16735"
     CDS             complement(13128..14501)
                     /locus_tag="VF226_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEX7075686.1"
                     /translation="MTARVLVVDDIPANVKLLEARLSAEYFDVLTACSGREALEVCSR
                     ERVDVVLLDVMMPGMDGFEVCRRLKSNPATQHIPVVMVTALDQSSDKIQGLEAGADDF
                     LTKPVDDIALITRVRNLGRLKMLNDEILMRAETRERMGLGPAECLADAVGPEGGHILL
                     VDDSRRTAQRMVDMLARAGHQTVVESDLQAALIAAAEQKFDLLIVSLSLANADGLRLC
                     SQVRSLDRTRHLPIIMIVDPSDEGRLLRGLDLGANDYLIRPIDRNEMLARVRTQIRRK
                     RHSEQLRTRLAESVELSIKDALTGLFNRRYMETHLKALVEQSTATGRPLSILVADIDL
                     FKRINDTHGHAAGDQVLQEFANRFRRNTRGVDLVCRLGGEEFVVVMPNTDIRRASMIG
                     ERLRGCIAGEPFRTESGHSLAITASVGVASVESAADTPATLFRRADSALYMAKRAGRN
                     RVVVEAA"
     gene            complement(14510..14911)
                     /locus_tag="VF226_16740"
     CDS             complement(14510..14911)
                     /locus_tag="VF226_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7075687.1"
                     /translation="MRRSAKLVLIVEDNELNMKLFHDLLDAHGYATLQTRHGMEALHL
                     AREHRPDLIIMDIQLPEVSGLEVTKWLKDDDDLRDIPIIAVTAFAMKGDEERIREGGC
                     EAYISKPISVSGFIDTIRRFIGESRLPSSSA"
     gene            15113..15403
                     /locus_tag="VF226_16745"
     CDS             15113..15403
                     /locus_tag="VF226_16745"
                     /inference="COORDINATES: protein motif:HMM:NF023518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HEX7075688.1"
                     /translation="MRSAPQMPLEEAEVIGLKALAFLAEDEARLSRFLALTGLRGDEL
                     RARAADAATLWAVLNHLAGDESMLLVFAASASLPPERIGPALQLLASQADGT"
     gene            15400..16326
                     /locus_tag="VF226_16750"
     CDS             15400..16326
                     /locus_tag="VF226_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7075689.1"
                     /translation="MTANLQERIRIGIDLGGTKIAAAALLPDGQMTEELRLPTPRNDY
                     EATLESIRQLVEQLEANTGERGTVGVGMPGSISPATGLVQNSNSTWLNMQPFDRDLSQ
                     RLGRRVRLANDANCFALSEATDGAAAGARTVFGVILGTGCGGGIVVNGELLNGPRGIA
                     GEWGHNPLPWPEPDEYPGPHCWCGRRGCIEKWLSGPALEEDHHRVTGERLTAVEIGRR
                     AAAGDSASRDTLARHASRLARGLAHVVNILDPDVIVLGGGLSQLKHLYDVVPRLMADH
                     VLAHNAAVDIRPPKWGDASGVRGAAWLWGHAR"
     gene            complement(16394..17263)
                     /locus_tag="VF226_16755"
     CDS             complement(16394..17263)
                     /locus_tag="VF226_16755"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEX7075690.1"
                     /translation="MGIGPRLVAAGLAWSALAAFVPAGAAEAIGLTPHRAVYDITLVD
                     ARPGAGISELTGRMVYEFTGAACSGFTQKMRFVTSTTNQEGETSITDMRTSSWEDAAG
                     DRFRFENSQYRNQQRVEQTVGEAVRRKTEIRVELKQPKKKTIKLPNAAIFPIQHSSRM
                     LEAAREGKHIFTTDLFDASEKGEKVYATNAYIGSRLPPGYNKQLPEVPGADVLNELPA
                     WPVALSYYEKGTEQEDALPAYELSFIYFDNGVSRRLLIDYGNFSIRGELKELTILEPE
                     PCASSQSSGRKTN"
     gene            17402..18178
                     /locus_tag="VF226_16760"
     CDS             17402..18178
                     /locus_tag="VF226_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX7075691.1"
                     /translation="MFERGGVSLGRGFLRPIAHRGLHDAAEGIIENTAPAFEAAISRG
                     FGIECDLRPASDGTPFVFHDSTLDRLIDAEGPIAAREPAQLSRLCYRGQSTRIISYSD
                     LLDLVAGNVPLLVEIKSDWDEPNPAFLSAVAELSMAYRGALALMSFDPAVITALRHLA
                     PTIPRGIVSGSYEGPGWCRDRIDAERAYRLSHCLEAGPAEPSFIAYHVDDLPTPVTRF
                     AREVIRLPVFAWTVRTAAQLRHAAAWADAPIFEGCDPCPA"
     gene            18310..18630
                     /gene="sugE"
                     /locus_tag="VF226_16765"
     CDS             18310..18630
                     /gene="sugE"
                     /locus_tag="VF226_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX7075692.1"
                     /translation="MAWVVLFIAAGLEVAWAIGLKYTHGFTRLWPSVLTLLAMATSMY
                     LLAIAARTLPIGTAYAVWVGIGATGTAILGMVLFSEPANAARIACLGLIIAGVLGLRL
                     LEGG"
     gene            18717..19907
                     /locus_tag="VF226_16770"
     CDS             18717..19907
                     /locus_tag="VF226_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7075693.1"
                     /translation="MKRDEAAVVRVTTSIAEIDGNAWDACANPDAKGFNPLVSHAFLK
                     ALEEAGTVGRRAGWLPQHLVLEGPAGHIAACMPLYLKSHSRGEYVFDWSWADAYERAG
                     GSYYPKLQSAVPFTPVPGPRLLVPPGPDRDERERTLALAAIALAERLRVSSLHITFPT
                     EGEWRRLPELGFLQRTDQQFHWHNEGYRSFEDFLASLASRKRKAIRKERAQALAPGIS
                     VELLTGSDIHERHWDAFFAFYMDTGSRKWGRPYLNRRFFSLLGEAMADRCLLVMAKRN
                     GRYVAGALNLIGGDCLYGRYWGAIEHHPCLHFEICYYQAIDFAIARGLPRIEAGAQGE
                     HKLARGYMPVPTYSLHWIADRNFRRAVARYLEEERREMAEHRELLAAYGPYRKAAAAS
                     EETF"
     gene            19937..20359
                     /locus_tag="VF226_16775"
     CDS             19937..20359
                     /locus_tag="VF226_16775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019565530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX7075694.1"
                     /translation="MTYDDNNVFAKILRGELPAHKVYEDEHTLAFMDIMPRCKGHTLV
                     IPKTPARNILDATPEQLATCMITVQRLAAAVMKAFDATGVTIQQFNEPAGGQVVFHLH
                     FHVLPRWEGVHLGPHTGKMEDNEVLAANAEKIRQALAS"
     gene            20444..21088
                     /locus_tag="VF226_16780"
     CDS             20444..21088
                     /locus_tag="VF226_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075695.1"
                     /translation="MACILVIGASRGTGLETVRKALAAGHHVRALARSAASIPIVDER
                     LEKITGDATDRTALLNALEGIDAVIVTLGVSPSLSTSLSGTRLFSTATRALVDTMKEK
                     GVRRLVAITGIGAGESRGHGGPLYDWVIFPLMLKRVYDDKDMQEQIIRRSGLDWTIVR
                     PGLLTNGPETGRYRALLDPAQWRADSISRADVADFLAKEVTERKYVGQAPVLVG"
     gene            complement(21180..23627)
                     /gene="clpA"
                     /locus_tag="VF226_16785"
     CDS             complement(21180..23627)
                     /gene="clpA"
                     /locus_tag="VF226_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX7075696.1"
                     /translation="MPSFSQSLEAALHRALEYANERKHEYATLEHLLLALIDDRDAAG
                     VMRACNVDIDALRNRVTEYLDTELGSLQVSGPSEAQPTTGFQRVIHRAVVHVQSSGRE
                     EVTGANVLVAIFAERESHAAFFLQEQEMTRFDAVQYISHGIAKRPGMSDPRPARGVDE
                     DTTEGDGKKPGADALNTYCVNLNKKAREGRIDPLIGREAEVLRTIQVLCRRQKNNPLF
                     VGDPGVGKTAIAEGLARKIIRGEVPDVLKNATIFALDMGALLAGTRYRGDFEERLKAV
                     MKEIENYPGAILFIDEIHTVIGAGATSGGAMDASNLLKPALQSGTVRCIGSTTYKEYR
                     QHFEKDRALVRRFQKIDVKEPTIEDSIEILKGLKPYFEEYHKLKYTNEAIKAAVELSA
                     KYIHDRKLPDKAIDVIDETGASQMLVPEGKRKKKITVKEVEATVATMARIPPKTITKS
                     DAEVLSNLERDLKRLVFGQDRAIEALASAIKLARAGLREPEKPIGCYLFSGPTGVGKT
                     EVAKQLANLMGVELLRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGLLTDGVDQHPHCV
                     LLLDEIEKAHPDLFNILLQVMDHGKLTDHNGKQVDFRNVILIMTTNAGASDLAKAPMG
                     FNRARREGEDQEAINKLFTPEFRNRLDAVIPFSGLPPEIIFKVVEKFIFQLEAQLADR
                     GVTIELSEAATKWLAEKGYDDKFGARPLARVIQEYIKKPLADELLFGKLEHGGTVKVL
                     VTGEGADQKLDFEIIPGEPKPPKAKDEDEEDEDLEDEAEKALIEAAVPRKALPGPKEK
                     RDRKSTGTVPTVPRKPD"
     gene            complement(23635..23970)
                     /gene="clpS"
                     /locus_tag="VF226_16790"
     CDS             complement(23635..23970)
                     /gene="clpS"
                     /locus_tag="VF226_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX7075697.1"
                     /translation="MAGNNGPDRGSEEDGRTGLVTKTRPKTKRPHLYKVLLLNDDYTP
                     MEFVVHVLERFFNKGREEATRIMLHVHHKGVGMCGVYTYEVAETKVTQVMDFSRQHGH
                     PLQCTMEKE"
     gene            complement(24243..24557)
                     /locus_tag="VF226_16795"
     CDS             complement(24243..24557)
                     /locus_tag="VF226_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX7075698.1"
                     /translation="MMNSVDVQKLGQENLDRAMRLLGDWNKNWQAIAAEMTDYSKRSF
                     EQSTKTFEKLLAAKSLEQAIEIQTSYARRVYDEYMQQMTKLGGMYANLAKEAYKPLDT
                     GR"
     gene            24953..26536
                     /locus_tag="VF226_16800"
     CDS             24953..26536
                     /locus_tag="VF226_16800"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7075699.1"
                     /translation="MRLDTFNLDLRRLHVGFCLLAAMLVALSWPGEASASNRRASMVI
                     DANTGKVLHASNADAPRHPASLTKMMTLYLVFEEIESGRLSLDSKIKMTKWGAMAPPS
                     KLGLKEGEEISVRDAIRALVTKSANDVARAIGEHIGGTEVNFAKMMTEKARLLGMSNT
                     TFRNASGLPDVEQVTTARDMLTLAMHLQDDFPQHYHFFSTRVFNFRGRSYRNHNALLR
                     TYPGTDGLKTGYIRMSGFNVVTSVRRGGRHVVAAVFGGKTAASRDAEMRALLNRTISK
                     GSTKKTRKPDAVSFYRATPRAPAKRPEPAAEPTVVAASTAPPVPTRAAEAPPVSADST
                     SKAPTISVAKVRSVPVAPMAAQENEPVLVQASAGRVATSRPPGAMALGRKPSTLDAQA
                     EALQQEQPAVERVAFVDASALSPAPSPAPVTRAAPSAEAAPKTASQEASKSAREGSYV
                     LQVGAYSTAAEAKKALVATLERAPALLAEASPVTVPVKAGAKQLYRARFTGFDPAAAR
                     STCEELRRRSIDCFVTKAD"
     gene            complement(26550..27236)
                     /locus_tag="VF226_16805"
     CDS             complement(26550..27236)
                     /locus_tag="VF226_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7075700.1"
                     /translation="MQYFLLGLAALLLVLLALRGFTRANVSAMARQIRIVTGVVALAA
                     AAALALRGLLSWAGPLAAFGTWMLFGGGGAPLRQPNKSPGQSSRVVTDYLEVELEHDT
                     GAMRGRVLKGTFAGREFETLAPAELAMLWQECRFSDMQSAQIVEAYLDSVYPSWRDDM
                     ARAGSAAAGGEDMTREQAYDVLGLAPGASEEEIRRAHRELMLKLHPDVGGSSYLAAKI
                     NLAKDILLGR"
     gene            complement(27252..27887)
                     /locus_tag="VF226_16810"
     CDS             complement(27252..27887)
                     /locus_tag="VF226_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7075701.1"
                     /translation="MKAMTPHATARGRLVFAMDATMSRQPTWDMALGLQADMFKAVKE
                     VGGLDVQLVYFRGAGECRASKWVSDPDALARLMTTVSCQGGYTQIRKVLSHARQETER
                     RKINALVYVGDCMEESIDDLCGRAGELALLGVPVFLFQEGHDARAERAFREIARLTRG
                     AYCRFDAGSAAQLRELLSAVAVYAAGGRMALERLSHRSAGRGAQLLLAQMK"
     gene            complement(28170..31391)
                     /gene="putA"
                     /locus_tag="VF226_16815"
     CDS             complement(28170..31391)
                     /gene="putA"
                     /locus_tag="VF226_16815"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEX7075702.1"
                     /translation="MANQLIALHSGKRDAEQVIPPRDEIAAYHLMDEQRLVGALIERA
                     IFAEDERRRIGDLARRFVHAARAGRHKYSGIDAFMLEYGLTSEEGVILMCLAEALLRI
                     PDTETADKLIAEKIGGGQWEKHLGRSDSLFVNASTWGLMLTGQIVKLRDAKSERPTNL
                     LKRMVVRSGEPVIRQALRHAMRILGNQFVLGRTIKEALGRAAPMEARGYRFSYDMLGE
                     AAKTARDAERYFERYMLALDEVGRHAGPLVSEHPDALMARPSLSVKLSAIHPRYEPGK
                     EARLERELLPRIVELAAAARARGIAVTIDAEEQDRLDLSLELFARVLADPKLDGWHGL
                     GLAVQAYGKRAIPVLRWLRRLALRTGKRLPVRLVKGAYWDSEIKWAQERGFADYPVFT
                     RKLHTDVSYLACMRLLLSDPQAFYPQFATHNAHTVAAAFVSAGDAPYEFQRLHGMGEA
                     LYEEVVGRGKLNRPCRVYAPVGGHEDLLAYLVRRLLENGANTSFVNRLADEEAPVADI
                     IRDPVEMAESERELPPEARPSRLPRPPEIYGPARRNSLGLPLSEPTVRSALQAEMAEA
                     LQQPLAVGPIIGGETPAGGEELIATCPHDHRRELGTVRLADADMVEEAITRACAAARD
                     WDLRGGPARAEILEKAADLLERDRARLMAVIVAEAGKTLENAQADIREAVDFLRYYAS
                     EARRLFSGPTELHGPTGERNTLELRGRGPFACISPWNFPIAIFTGQVSAALAAGNPVL
                     AKPAEQTPMTAYFTTRLLHEAGVPPDVLHLLPGAGDIGAMLVKDTRIQGVAFTGSNET
                     GWHIQRALADRRAAIVPFIAETGGMNAMIVDSSALPEQVVRDAVRSAFDSAGQRCSAA
                     RVLFVQEDVAKRVIDMLVGAVEELDVGDPFDYGTDLGPVIDETAQDTLDAHKARMYRE
                     AKVLADLKLPEKCRVGTYVTPAVFEIDHIARLEREVFGPILHVIRYQQGHLGEVIDAI
                     NATGYGLTLGLHSRIEAVADFVAERARVGNLYVNRNQIGAVVGVQPFGGEGLSGTGPK
                     AGGPHYLPRFATERVRSTDITATGGNVQLLGLGLDTSLATPRQ"
     gene            31607..32443
                     /gene="panC"
                     /locus_tag="VF226_16820"
     CDS             31607..32443
                     /gene="panC"
                     /locus_tag="VF226_16820"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7075703.1"
                     /translation="MVVRSLAPLREVVGGWRSGGERIGLVPTMGALHEGHLALVRTAK
                     ARAGRVVVSIFVNPTQFAPHEDFTTYPRDEAGDLEKLAAIGCDLVWAPDVNVMYPPGF
                     ATSIVPKGPAEGLETDHRPNFFVGVATVCTKLFTQVAPDLAVFGEKDYQQLCVIRQVV
                     KDLDLPLEIVAHPTVREPDGLAMSSRNAYMTAEERARAPIIHKVLLQTAEAVASGQGI
                     AAATSCGREELVAAGFKVDYLEVRDAETLAPVEDGSKRPLRVLAAAWLGKTRLIDNVA
                     VG"
     gene            32638..34653
                     /gene="parE"
                     /locus_tag="VF226_16825"
     CDS             32638..34653
                     /gene="parE"
                     /locus_tag="VF226_16825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX7075704.1"
                     /translation="MAQSQRDLFARLVDEMNHKTARARRVIDASDTYTAADIEVLEGL
                     EPVRRRPGMYIGGTDERALHHLFAEVIDNAMDEAVAGHASWIDVHLDADGFLTVTDNG
                     RGIPVDPHPKFKDKSALEVIMTTLHSGGKFESKAYNTSGGLHGVGVSVVNALSETLEV
                     EVARGQQLYRMVFSRGEPQGKLQRLGSIMNRRGTKVRFKPDEKIFGKGVAFKPARLFR
                     MARSKAYLFGGVEIRWSCDPSLIKDDTPAEAVFHFPGGLKDYMLATINGRTLVTKDMF
                     TGCVEKEGRHGSVEWAIAWMADEDGFSSSYCNTIPTPEGGTHEQGLRQALTKGLRAYG
                     ELVGNRRAAQITADDVMSTAASMLSVFIREPEFQGQTKDKLATQEAARIVETTVRDAF
                     DHWLAESPAQATRLLEWTIEQAEQRLKRKREKEVSRQSATRKLRLPGKLADCTIQSAK
                     GTEIFIVEGDSAGGSAKSARERETQAVLPLRGKILNVANASSAKLAQNQLLSDLILAL
                     GVGTGSKYRDEDLRYERVIVMTDADVDGAHIASLLITFFYQQMPQLIDNGHLFLAVPP
                     LYKITQGTQSAYARDEKHKEELLQTVFNGRGKIEISRFKGLGEMNAAQLKETTMDPKR
                     RTLLRVEIGDGDRSEIAEAVNNLMGSKPEARFRFIQERAAFAKDLDI"
     gene            34881..35144
                     /locus_tag="VF226_16830"
     CDS             34881..35144
                     /locus_tag="VF226_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075705.1"
                     /translation="MSRILAALAVALSLSLASTGVIAENAKAPAQKASTPKVLKAGKG
                     EKVCRYRFPNGEQKSWICKQEEPCCAWDLFSYVKCGSTITGCL"
     gene            35871..36782
                     /locus_tag="VF226_16835"
     CDS             35871..36782
                     /locus_tag="VF226_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7075706.1"
                     /translation="MHLSFHTLDVFTDRPFSGNPLAVVLDADRLTTEQMQSIAREFNL
                     SETVFVQKPANPAHTAKVRIFTPGAELPFAGHPTAGAAVLLAELRAPEAADHDAIIVL
                     EEAIGIVRVGVRLRRGEASFAEFDTPKLPQESGSMPPVEQIAAALNLLPREIGFANHR
                     PTCFAAGNSFGFVPVASLDAIARAAVNSQHWGAAFSNRGLTGAFLYCRDTVHTTSSFH
                     ARMFAPDFGVPEDPATGSAAVIFAGVIQRFDALPDGHHKRVLEQGFEMGRPSIISLTL
                     EVANGELHNVRIGGNAVRVTEGRISTP"
     gene            36798..37283
                     /locus_tag="VF226_16840"
     CDS             36798..37283
                     /locus_tag="VF226_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075707.1"
                     /translation="MDQKKLVTAAIALFSLAGLQLSPAAAEQVEWFGTRSEDGASLVY
                     GTPNSGYGKIVLSCAAGQEDLNFVYEHEPIDPKNGVQVEVLLSAGGVETSIPTTGTRL
                     EMDDVFILEGQTKLDDRLRKILTSPGSLILTVEDGTAEFPLDGAANAARDLLEVCSPE
                     G"
     gene            complement(37547..37915)
                     /locus_tag="VF226_16845"
     CDS             complement(37547..37915)
                     /locus_tag="VF226_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075708.1"
                     /translation="MNFQIPEWVKPAVWGAVVGAVAIAIVGFAAGWVVTSGAAEQMVS
                     KEKKQAVIAALTPVCVAQFKSLTQQQQQVQLAALKGESSWKRGDFVEEQGWATMPGTT
                     EPNGEVADACASELMKVAGN"
     gene            38691..40274
                     /locus_tag="VF226_16850"
     CDS             38691..40274
                     /locus_tag="VF226_16850"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX7075709.1"
                     /translation="MSQTTNPGDQVLIFDTTLRDGEQCPGATMTFEEKLEVADLLDQM
                     GVDIIEAGFPIASDGDFEAVAAIAKRVKRAVVAGLSRAGFKDIDRCGEAVKYARRPRI
                     HTFISTSPIHMKYKLQKEPEEVLEMVRASVERARNHVDDVEWSAEDGTRTEIDFLCRC
                     VEIAIKAGATTINIPDTVGYTTPEEYFQLFTTVRERVPNSDKAVFSVHCHNDLGMAVA
                     NSLAGVKAGARQVECTINGIGERAGNAALEEIVMAIKTRNDVLPYTTGIETTMLTRAS
                     KLVAAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGVSKTSLVM
                     GKHSGRNAFRTKLKELGYDLGENALEDAFNRFKALADRKKVIYDEDIEALVDEGIATA
                     TDRIKVVALTVIAGTSGPQSATLTLDIDGKHQTVQATGNGPVDATFNAIHALVPHTAV
                     LELYQVHAVTEGTDAQAEVSVRLHENGYTSSAKAADPDTLVASAKAYVAALNKLLIKR
                     QRGAPAQHFAEPAANLRGV"
     gene            40584..41627
                     /locus_tag="VF226_16855"
     CDS             40584..41627
                     /locus_tag="VF226_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX7075710.1"
                     /translation="MLPKFSGVFSEDIAIDLGTANTLVYVLGRGIILDEPSMVAIRSS
                     AGEREMVAVGAEARRIIGLTPEPLEALCPLRDGVIADFLATEFMLRQFIRRARSILNF
                     RRPRILISVPAGATPVERRAVVETARSAGARTVALIAEPVAALLGAGVPVYDATGSMV
                     VDIGGGTTDIAIVSYGKVITARSLRCAGNAMDEAIIRYVRRRHHLVIGEASAERIKIE
                     AGSASAVANGRDADIHIRGRDLRRGRLKSVVLGPPHIAEALDQPISEITEFAQRALEE
                     VPAEIAASIYERGIVLTGGGALLDRLDHELTRRLGIPFALAKDPMRCVIMGTAIVLEH
                     LADSGHLLLSPDF"
     gene            41754..41829
                     /locus_tag="VF226_16860"
     tRNA            41754..41829
                     /locus_tag="VF226_16860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41787..41789,aa:Lys,seq:ctt)
BASE COUNT         7491 a        14009 c        13225 g         7298 t
ORIGIN      
        1 cagtccgtag ccaaggcccg catgcagata aatgacgtag aatgagaaga acgcgctctg
       61 cacggtcgca aaaccggcgc aggcgagcgc gaggcggatg aggcccggcg ccagccccag
      121 cgcacggaac ggccgctgca agttggacgg cgagaagatt gccgagaggc ggaacgcctt
      181 cagcgggcgc gtcgtgttgt aggagcgcgg gacaaacagc agcaccaggg cgagcgtcag
      241 gcagatcagt ccgatcaatg cgaaggccag ccgccaaccg aactgcgcga tcagcaggcc
      301 gccgagcccg cccgagacga cgccgcccag cggaacgccc gcctgccgca gggaaaacag
      361 catcgaccag ttcgccttcg gcgtctggtc ggcgaggatc tggctcccgg caggcgtcga
      421 ggtcgagtag ccgaagccga gcaagacggc gccgatcacc atcagcggca gataggctgt
      481 catggcgagc gcagcaccgg caagtccgat gagaattccg accctcagcg tgcgcaccgg
      541 cccgagtgcc ggcgtgatgc cgtggttgga catatagagc cagatggagc cgatccccgc
      601 ggcccccgcg atccagccgt aagccgtcgg atcgacgccg gctgcggcca tcatctccgg
      661 ggcgacgacc ggaagaaaca gatggagcgc cgacatgccg acaagcggca ccagcaccac
      721 gagcagcggc gtcaaatagg gcggcatctg gggaaatgaa ctccggaaat tgaggcaacg
      781 tcagtctttc agctccgacg cgatcgggca agcgcacgag gtgccttttt ccgcccccgt
      841 catttcccca tacgccaaaa tcgcagccac gcgatctttg atgccgaggt tggtaaccga
      901 gctgggcaga accgccatct cggcttttcc cgaccggagc cggaaccggc ccctcacggt
      961 gatgttcccc tgtcgacggc ctcaccaaat ccgcttgccg ggttccccaa ctgctcctat
     1021 agaagcggcc ttgatgacca gcaccgcccc catcgctgaa gctacctcct tcccgcaccg
     1081 ccatcttttg acttgtgaga ccttgacggc agcggaaatc actgcgctcc tcgaccttgc
     1141 cgacaacgcc gccgagatca atcgccaggt caacaagaag cgggacgtgc tgaggggccg
     1201 aaccctcatc aacctgttct tcgaggcctc gacgcggacg cagagctcgt tcgagctagc
     1261 gggcaagcgg ctgggcgccg atgtcatgaa tatgaacgtc tccacctcct ccgtgcagaa
     1321 gggtgagacg ctgatcgaca cggcgatgac gctgaatgcc atgcggcccg acatcatcac
     1381 cgtgcgccac tacgcggccg gtgccgtcga gctgctggcg cagaaggtcg actgctcggt
     1441 catcaatgca ggcgacggcg cccatcagca tccgacgcag gccctgctcg atgcgctcac
     1501 catccgccgc gccaagggca ggatcgccgg gctgacggtc gccatctgcg gcgacattct
     1561 ccacagccgg gtcgcgcgtt cgaacctcaa cgtgctgaac acgatgggcg cccacgtgcg
     1621 gctgatcgcg ccctccacgc tgctgcccgc ggagccggag cgtttcggcg ccgaggtgtt
     1681 cacggacatg tggaagggcc tcgagggggc ggacatcatc atgatgctgc gcctgcagcg
     1741 ggagcgcatg cacggctcgc tggtgccttc gagccgcgag tacttccact tcttcggcct
     1801 cgaccacgag aagctggcgc gcgccaagcc cgacgctctc atcatgcatc ccggcccgat
     1861 gaaccgcggc gtcgagatcg catcgaccat cgccgaccac tcccgcagtc tgatacggga
     1921 gcaggtcgag atgggcgtgg ctgttcggat ggccgtgctc gaggcgctgg cccgcaacct
     1981 tcccaaccga tgagccggcc gatgcgctcc gccaattcga agatcgcagc cacgacccgg
     2041 cgtacggttt tcatcaacgc gcgcctgatc gatccggctt cgaaccgaga cgagccggga
     2101 gggttgctgg tcgccgatgg cgaaatcgca gacctcggcg ggcacctgcg ccgcagcgcg
     2161 ccggaaggcg ccgacgtgat cgactgcaag ggccatgtcc tggcccccgg tctgatcgac
     2221 atgcaggtgt tcatcggtga gcccggcgcc gagcaccggg aaacgctcaa aaccgcgagc
     2281 caagcggccg cggcgggcgg ggtcaccacg atcgtcgtga tgccggacac caatccggtg
     2341 atcgaccagg tcgccctcgt cgacttcatt cagcggcggg cccgcgacaa cgccgtcgtc
     2401 aacgtccaca ccatggcggc gctgacgaag gggctcgaag gccaggagat caccgagatc
     2461 ggcctcttga agcgcgccgg agcgatcgcc ttctccaacg gcaagaacac gatcgccaac
     2521 acccgggtga tgcgcaacgc cctgctctac ggcaaggact tcaacacgct catcgtgcat
     2581 cacacggagg atccctacct caccgagggc acgtgcatga atgcgggccc cgttgccgcg
     2641 cgcctcggcc tgctcggcgc acccaaggcg gccgaggcca tcgtgctgga gcgcgacgtg
     2701 cgcctcgtgg agatcaccgg cggccgctat cacgcggcga ccatctcctg ccgggagagc
     2761 ctggatatcc tgcgtgcggc caaggcccgc ggtttgccgg tcacctgcgg ggcctcgatc
     2821 aatcacctga cgctgaacga gaacgacatc gggtcctacc ggaccttctt caaactccgc
     2881 ccgccgctga gggccgagga ggaccgcgcg gcgctcgtgc gcggactggc ggccggcgac
     2941 attgacgtga tcgtatccag ccacgacccg caggacgcgg acgtgaagcg ccgcccgttc
     3001 gccgaagccg ccgacggcgc catcggcctc gaaacgctgc tgtcggccgc cctgcggctg
     3061 cattacacgg acggcgtgag cctgatgggg ctgctgcgcg ccatgaccat caatcctgcg
     3121 cggcttctgg gcctgccctc gggacgcctg gaaaagggcg ccccggctga cctcgtcctg
     3181 ttcgacatgg cccagccctg ggtggtcgat cgcgaggccc tgcgctcgcg ctccaagaac
     3241 tctccattcg acgaaacgaa gatgcaggga cgtatcctgc tgaccatggt tgaaggcgag
     3301 acagtgtatc gataccctga atcgcagcgg ggctgaggag gcgccgcaac gctccgcgca
     3361 gtgatcaccc catgatggac atcgcgcccg ccacgctcgt tctagcgatc gcaattggtt
     3421 atgccctggg ctccattccg ttcgggctgc tgctgacgcg gcttgccggc ctcggcgata
     3481 tccgtcacat cggctccggc aacatcggcg ccacgaacgt attgcggacc gggaacaaga
     3541 gtcttgcagc cgccacactg gtgctcgatg cggtgaaggg aacggcaggc gttctcgtcg
     3601 gcagtgcgct cggcggtccc ttggctgctc tggcaggggg cctcggcgcc ttccttggac
     3661 acatctttcc cgtctggctc aggttcaagg gcggcaaggg cgtcgccacc tacatcggca
     3721 tcctgctcgc gctcagttgg ccggcttgcc tggtgttctg cgccatctgg ctcgccgtcg
     3781 cggtgctgag ccgctactcc tcgctctccg cccttgtggc cagcatcgtc gcgccgctcg
     3841 tgctttggct cctcggcgag ggccagccag ccgcgctcgt cggggtgctg agcgtgctgc
     3901 tcatcatcaa gcatcatgcc aacattcagc ggctgatcgc cgggactgag agcaaaatcg
     3961 gtgcccgggc ctgacgacaa gccaaggccc aagccaccgc cgctgccgac ggcccgcctc
     4021 gacgacagcc agcgcctcgc ctgcctgcgc ctgatccgct cggaaaacgt cgggcccgcg
     4081 acgttccggg ccctcatcaa tcatttcggt ggggcgcgcg cggcgctgga ggccctgccc
     4141 gagctgtcgg cgcgcggcgg acgacggacc ggcatccgga tttgctccca ggagtcggct
     4201 gaacgcgagc ttgcagcggc agagcgcatc ggggcgcggc cgctctttac gatcgagccc
     4261 ggatatccgg cggctcttgc cgtcatcgac ggcgcaccgc ctctcattta cgtgaaggga
     4321 gatgaccggc tgctcgcccg cccagccgtc ggcatcgtcg gatcgcgcgc gtcctccgcc
     4381 gccggccagc agctcgcgcg ccaactcgcg gcggggcttg gacaggccgg ctacgtcgtg
     4441 gcctcagggc tcgccatcgg gatcgatgcg gctgcgcatc aggcatcgct ggagacaggc
     4501 accatcgccg tattggccgg tggaatagac gtcgtatatc cgccgcagaa tgcggctctt
     4561 caccacgcaa tcggcgaaac gggttgcctc gtgagcgagc agccgccggg tttccagcca
     4621 aggggcaagg atttcccgcg ccgcaaccgc atcatctcgg gcctctcgct cggcgtggtc
     4681 gtggtcgagg cggcgcgccg ctctggctcg ctcatcacgg cacgccgggc ggccgaccag
     4741 gggcgtgagg tgttcgccgt gcccggacac ccgctcgacc cgcgcgcggc cggcacgaac
     4801 caactcatca aggaaggcgc cacactcgtc acctgcgccg aggacgtggt gaatgcgctg
     4861 gagccgttcg gtcggcggcc gtatcctatg gagcaggcgc gaaccttcga ggagtcagcc
     4921 tcggccccca atgccacacc ggagccgctg ccggacatcg cgcatgatga gaggtccctt
     4981 gtcgtcgctg ccctcggccc ttcgccggtt cacgttgacg agcttgcgcg ggcaacgggg
     5041 cttccgatcc ggatcgtgca gaccgtgctg ctcgagctgg ctctcgccgg ccggatcgag
     5101 caccacggca gccaactggt ctcactgatc gccggacggg attgagccag ccgctgccag
     5161 tccggttcgc cttccaacgt catcccggtg cttgtcaccg ggatccacct ggttgctggc
     5221 tccggcgctc gctgtcggat ggatcccggg cacaaggccc gggatgacgt ctcgattcgc
     5281 aaggcaacct cggctcgaaa cccggctcag ccaggcccga tccagctcag gcgccgcgga
     5341 agagcccgcc tgtgctggtc ggcctcacgg tgcgccagct ccagcagctc ggccaccctg
     5401 gaacacccag tctgccaagc caggacctgc atatgtcgga tcatatctgc caggtactcg
     5461 aattgctccc gcgtgggtcc tggttcggtt tgagccggct ggagcgcctc ccgctcccca
     5521 cgaacgagcg caagcctgca cttgccgtcc cgggcctgtc ccatcacgat cacccccacg
     5581 tctcggctcc acgacccctc gtcgtgccgt gtttgccgac actaacgcat ctttgataga
     5641 aaatctacga aatttgcata ttgtcctaat ttgacttccc cacggccatc acccatcttc
     5701 ccccacctga atgtcaacga cggacctggc gcggccccgc gcacgtgcct gttgcgggcc
     5761 atgcaaagga tcgccgccgc tgggatcccc ggaaagcaca tgaacattgt cattgtagaa
     5821 tcggctgcga aggccaagac gatcaacaaa tacctgggct cgaactacaa ggtgatcccg
     5881 tccttcgggc acgtgcgcga tctgccttcg aaggacggct cggtcgagcc cgaccgcgac
     5941 ttcgccatgc actgggacgt cgatcccaag gcggccaagg tgatgcggga gatcgctgac
     6001 gcggtcaaaa aggcggacaa gctgatcctc gccaccgacc cggatcgcga aggcgaggcc
     6061 atctcttggc atatcctgca actgctcgat cagaagcggg ccctgaagaa gggcgtgccg
     6121 gtcgagcggg tcacgttcaa cgcggtcacc cgcgacgcgg tgctcgccgc catgaagaac
     6181 ccgcgtcaga tcgatgcgcc tctggtcgaa gcctatctgg cccgccgcgc actcgattac
     6241 ctcgtcgggt tcacgctctc gccggtgctc tggcgcaagc tgccgggcgc ccgctcggcg
     6301 ggccgggtcc agtcggtggc cctgcggctc gtctgcgatc gcgaagccga gatcgaggcc
     6361 ttcaagtccg aagagtactg gacgatcgag gcattgctcg ccacgcccaa gggcgaggag
     6421 gtgcgcgcgc ggctcgtctc catcagcggc cggcttctca agaagctcga catcaaggac
     6481 caggggagcg cgcacgcgat caaggctgcg atcgagggtg gccgcttcac cgtatcctcg
     6541 gtggagaaga aggcggtacg ccgcaaccct gccgcaccat tcaccacctc gaccttgcag
     6601 caggaggcct cgcgcaagct tggcttctcc gccaaacaga cgatgcaggt ggcacagcgc
     6661 ctttacgagg gcgtcgatgt cggcggggaa acggtcggcc tcatcaccta catgcgtacc
     6721 gacggcgtcc agatcgtgcc ggaagccgtt tccgagatcc gccgcgtgat cgagggcgag
     6781 tacggcaagc gctacacccc cttcgcccgc gagtacaaga gcaaggccaa gaacgcgcag
     6841 gaggcgcacg aggcgatccg gccgaccgat ccgagccggc ggccgcgcga tgtcgcgcgc
     6901 tatctcgaga aggatcaggc cgctctctac gagctcatct ggtgccggac gatcgcgagc
     6961 cagatggctg ccgccgagct cgagcagacg acggccgaga tcaccgtcga aggcaaggat
     7021 ggcaagacct acggcctgcg cgccaccggt tccgtcgtcg tgttcgacgg cttcatgcgg
     7081 ctctactcgg aaggccgcga cgaccgcgtc tcatccaaag accaggtgga cctggaggac
     7141 gaggacgaca gccgccgcct gccgccgctg gagcgcggcg atgtcttgaa ggacaaggcg
     7201 gtcacggcga cccagcattt taccgagccg ccgccgcgct acacggaagc cacgctcgtc
     7261 aagcgcatgg aggagcttgg catcgggcgt ccctcgacct atgccacgac gctcgcggtg
     7321 ctgcgcgagc ggggatacgt gcggctcgat aagaagcgcc tcatccccga ggacaagggc
     7381 cggctggtta ccgcgtttct cgagagcttc ttccgccgct acgtcgagtt cgatttcacg
     7441 gccgacctcg aggagaagct ggacctcatc tccgacggca agctcgagtg gaaggacgtg
     7501 ctgcgtgact tctggcgcga cttcaccggc gccgtcgacg agatcaagga tctccgcgtc
     7561 agcgaggttc tggaggcgct gaacgaaatc ctgggcccgc acatcttccc gccgcgggcc
     7621 gatggcgtcg atccgcgcac ctgccccgta tgcgggtccg gccggctcag cctgaaggtc
     7681 gggcgcttcg gcgccttcat cggctgctcc aactatccgg agtgccgcta cacgcgccag
     7741 ttcaccgagc cccagggcga ggcggaggcg acgccacccg aaggcaagct gttgggctac
     7801 gatcccgata cgggtctgcc ggtgtcgctg cgcacaggcc gcttcggtcc ctacgtgcag
     7861 ctcggcgaag cggaggacgg ggagaagccg aagcgggctt ccattccccg cggcctcgac
     7921 gcggccacga tcgacctgga acgcgcgctc cagctcctgg ccctgccgcg cgaggtcggt
     7981 ctccatcccg agacaggcaa gccgatcacc gccggcatcg gccgttacgg accgttcgtg
     8041 ctgcacgagg gaacctacgc caacctcgaa agtctcgaag acgtgttcac gatcggcatc
     8101 aaccgggcgg tggcgttgct cgccgagaag gcggccggcg gcaagggaaa atggcagcgt
     8161 cccaaggcgc aggtcctcaa agacctcggc gagcacccgg aaggcggcaa ggtcgaggtg
     8221 ctctcgggcc gctacggccc ttatgtcaag cacggctccg tcaatgcgac gctgcccaag
     8281 ggcaaggacc cggccgaggt gacgctcgaa gaggccgtgg agctcctggc ggcgcgggcc
     8341 gcgaatggtg gggccaagcg gggcaagacg aaggctcctt cgaagaacgc ggctgcgggg
     8401 aaagccggtt cggaaaagcc ttcggccaac aagacctcgg ctcccgcaaa gaaagcgccg
     8461 gcaaagaaat cggccaagcc gcgctccacc aagacccgcg tgccggagcc ggcggcggag
     8521 tgaaaggaat cgtgagatgg tgaagtcgcc aaatcgtgag atcgtgaaga tcgggcgcgg
     8581 ctgcgaacga gtctacaact tcacgacttc acgacttcac gacttcacca ccatctcacg
     8641 gacctgacca cgtggcccgc aagcgcacag caaaagggga agtaaagacc aaaaccgcgg
     8701 gcggcctccc gaccaagggc gagatcctgg acttcatcaa gggtgcgcag gagaaggtcg
     8761 gcaagcgcga gattgcgcgg gccttcggca tcaaagggag cgcgcggatc gagctcaacc
     8821 gcctgcttgc cgaactgagc cgcgaaggtg ccataacggg cctgcggaag gaggtgcgcg
     8881 ccaggggtgc gttgccgccc gtttcggtgc tggagatcgt cggccgcgac gaggacggcg
     8941 atctgttcgc cgagccggtg gtctgggacc gcacggaggg cgtccgcccc aaggtgctgc
     9001 tcatcaagag ccgcaggttc ctggatgctg gcgaaggtgg gttggggatc ggcgaccgga
     9061 tcctcgcccg tgtccggcag ctcgagggtg cggacatcga aggcttccgc ttcgagggcg
     9121 agccgatcaa gaagctgccg cgcgagaagc gccgcctgct cggcgtctac cgcgccaaca
     9181 agaaggatgg cggcgggctc atcgccccca tcgaccgcaa gcagctgaaa gactggctcg
     9241 ttcaaccggg cgatgaaggc gaagcgaagg acggcgacct cgtccggttc gatctgccgc
     9301 gcgtcttccg gtatggcatc ccgcaggcgc gggtgctcga atcgctcggc aacccgcagg
     9361 accagcgcca gatcagcctg atcgccgtgc atgcgcacgg cattcccgac gattttccgg
     9421 aaggcgtgct cgccgagctc aaccggctgg agccgccacg cgccgatggc cgggtcgatc
     9481 tccgcgccgt gcccctcgtc accatcgatc ccaaggatgc gcgcgaccac gacgacgctg
     9541 tgcacgcgga gccggacccc gatccgaaaa atcggggtgg cttcatcgtg acggtcgcga
     9601 tcgccgatgt cgctcactac gtccgcccgg ggaccaggct cgatcgcgag gcacagatcc
     9661 gcggcaactc ggtctacttt ccggaccgcg tcgtgccgat gcttcccgag cgtatctcca
     9721 acgacctctg ctcgctgcgg gagcatgagg agcgcccgtg cctcgccgtg cgcatggtgt
     9781 tcgacagccg cggcgagaag cgccgtcaca cgttcgtccg ggggatcatg cggtccgccg
     9841 ccaagctcag ctacgaggag gcacaggccg cattcgacgg caagccgtcg cggaaatgcg
     9901 ctgcactgat gggcaccgtg ctcgagccac tgtgggcggc ctatcaggtt cttgccgcag
     9961 cgcgggcgcg ccgtgagccg ctcgatctcg atctgcccga gcgaaagatc gtcctcgacg
    10021 ccgaaggccg cgtggagcgc gtggtcatcc cggagcggct ggaagcgcac cggctcatcg
    10081 aggagttcat gatccaggcc aacgtcgcgg ctgccgagac gttggagcag aagcggacgg
    10141 ccctcatcta ccgcatccac gcgccgccct cgaaggagaa ggttactgcg ctgcgcactt
    10201 tcctcgacac gctgcagctg cgcctgcccc cgggcgagtt gaagccggcc cacttcaatc
    10261 gcgtactggc gcaggccaag gctttgccca cggccgacct catcaacgag gtcgtgctgc
    10321 gcgcgcagtc acaggccgaa tacagcatcg acaacatcgg gcatttcggg ctcaatctgc
    10381 gccgctatgc ccatttcacc tcgccgatcc ggcgctatgc ggacctcgtc gtgcaccggg
    10441 cgctggtccg ggcgctgcat ttcggccccg acggattgac cgatgaggaa gccgaacgcc
    10501 ttgccgaggt agcccagtcc atctcggaag ccgagcgccg ggccatggcg gcggagcgcg
    10561 agaccatcga ccggctgatt gcggctcatc tggccgatcg ggtcggtgca gagtttgagg
    10621 cccgcatctc cggcgtcacc cgctccggcc tgttcgtgcg gctgaaggac acgggtgccg
    10681 acggcttcgt gccggtttcc tccatgatcg gagacttcta ccacctggta gaggagcaac
    10741 atgcactggt cggcagccgc agccaggaag cctatcggct cggcgacgaa gtccgcgtcc
    10801 ggctggtcga ggccattccc acggcgggcg cccttcgctt cgagatgctg tccaagggca
    10861 agcgcggccg cgtcctgctt acaaaaggct ggcgaggcgc ccgcacgatc cgcgggcggc
    10921 ggcgcgctgc actatgatgc tctccgcccg gagcgatgtt tggcggggtt gacgcacggg
    10981 ccaatacggt cccctattca gaacgaacaa gctcgcgagc agccgagcag gatcatgagg
    11041 acacgagcag atcatgacag tcaccatcgg gcgtaagcgc aatctgtatg acgcgattct
    11101 gcgtggcgcc cggcacaagt gcccggcctg cggcattggc cggctgttcc gcgcctacct
    11161 gaaggtggcg gatacctgcc cgcactgcca cgaggagctt tatcatcacc gcgccgatga
    11221 cgcgccaccc tacctgacga tcgtcatcgt cggacacgtc attgtcgccg gcatactggc
    11281 gttggagcag accgtcgctc cgccgcagtg gctgcaactg gcgctatggc tgccgctcac
    11341 ggtggtgatg tcgctcgtgc tgctgccgac catcaagggt tcggtcgtcg gcctgcagtg
    11401 ggccaacgag atgcacggat ttggtacgca tcccgacccg gacgagatcg agcatgaagt
    11461 ggctcgctcg ccgagctcca cctgacagac gccgaaacct gctccatgac cacgccaagc
    11521 acgccaccaa ccgggaccga tccggacaaa tcgatgcggc cgcgcgacgc agcgaccctc
    11581 gtcatcgtcg accgggtctt gggagtgcct cgcgtgctga tggggcaaag gcgtttcgat
    11641 caggtgttca tgccgggaaa gtacgtcttt cctggcgggc gcgtggacga aagcgatcga
    11701 gcggtcgaga gcgccgacga gctgcacccg gtcgaagtca gcaagctgct actcgacatg
    11761 aaaggccatc ccagccctgt gcgcgcgcgg gcactcggca tggccgccct gcgcgaaacc
    11821 ttcgaggaag ccggcgtcgt gatcggagca ccggccagct catcggccga aacgagatgc
    11881 gaggcgccca gttggaacgc gttcttcgcc tgcggctatc tgcccaagct gagctcgctg
    11941 aggctttttg cacgcgcgat cacaccgcca gggcgtcctc gccggtacga cacccgcttc
    12001 ttccatatct cggccgacga gatcagccac caggtcgaca cttccgatgg ggagctcctg
    12061 ggcctgcact ggctcaccat cgaggacgcg cgcagtctcg acctcccctc catcacgcgt
    12121 gtcatcctcg aggatttgac agatttcctc cgggatgggg ccagcggatc gggcgatgcc
    12181 ccggttccct attaccacca caagaacggc atctttcgcc gggacctcct gagcgtggct
    12241 gatgcctcga tgcaacttga caccgaaggc ggccaaggta tggtgcgccc gatttcaaat
    12301 gaacgcgcat gatgcgctga ggtctgcagg tcatggcgaa gccaatcacg cagaaaatca
    12361 agctgctctc gacggctggc accggctact tctacgtcac caagaagaac ccgcggacca
    12421 gtacggagaa gttgagcttc aagaagtacg atcccgtcgt gcgtaagcac gtcgaattca
    12481 aggaagccaa gatcaagtag tctggctcca cctcggcact ggtcgcgctc tcgcctttgc
    12541 ggagtcgcac caatccgccc ttcgatcaga agcggtcagc gatgctccgg gcctgagagg
    12601 ccatcggggt cgcgtcgttt tggtgtttca atggaatcag agcctcggtc agggcccgca
    12661 acgattctcg gcagcggggt agaagtccga gtacgtcgca gtccctgacc tgataaccaa
    12721 gcggctcgcc aaagagggcg ttggctcccc acttggtgag atcgaccaat tcgcagcctc
    12781 gaggaggcca actccagctg caggccgacc catgtccgag cgttcaggag atgcggcgga
    12841 cctgagacgc agcggacatt accgaactcg acgtggatct cagaggatgc cctatcgaaa
    12901 gatccaaacc gaccgagtgt aaccgtggta gattgtccca agtgcaatct tgacagatcg
    12961 ccaaatgctt tgcaagcagc tcgaaatgcg attcgctagc atcctcaaca ggctgcaaca
    13021 acgagtgaaa gttcgcctac aaccttaacg cgaatcttta atccagatcg cggctcacgg
    13081 gtagctccgc cgactggata actcgctcat atatctgcgt tttctgctca cgccgcttcg
    13141 acgacgaccc tgtttcgccc ggcgcgcttg gccatgtaga gcgcactgtc ggcccgtctg
    13201 aaaagcgtcg ccggtgtgtc tgccgcgctc tcgaccgacg caactccgac actcgccgtt
    13261 atcgcaaggc tatgcccgct ctcggtgcgg aagggctcgc cggcgatgca gccccgcagc
    13321 ctttccccga tcattgaagc ccggcggatg tcggtattcg gcatcaccac cacgaactcc
    13381 tcgccgccca gccggcagac gagatcgaca ccgcgcgtgt tgcggcggaa gcgattggcg
    13441 aactcctgca ggacctgatc gcccgctgca tgcccatgcg tgtcgttgat gcgcttgaac
    13501 aggtcgatat cagccaccag gatcgacaaa ggccgacccg tggccgtcga ttgctcgacc
    13561 agagccttga gatgagtctc catgtagcgg cggttgaaca gtccagtgag tgcgtccttg
    13621 atcgacagct cgacgctttc ggccaatcgc gtcctcagct gctcggaatg gcgcttgcgc
    13681 cggatctgcg tcctgacgcg cgccagcatt tcgttccggt cgatcggtct gatcagataa
    13741 tcgttggcgc cgagatccag tccccgcagc agccggccct catccgaggg gtccacgatc
    13801 atgatgatgg gcaggtgacg cgtcctgtcg agcgagcgga cctggctgca gagcctgagg
    13861 ccgtccgcat tcgccaggct gagactgacg atcagcagat cgaatttctg ctccgcggcg
    13921 gcgatgagcg ccgcctgcaa atcgctctcg acgacggttt gatgaccggc ccgggccagc
    13981 atgtcgacca tgcgctgcgc ggtgcggcgg ctgtcgtcga cgagcagaat gtgccctccc
    14041 tcggggccga cggcgtcggc gaggcattcg gcagggccga gccccatgcg ctcgcgcgtc
    14101 tcggcacgca tcaggatttc gtcgttgagc attttcaggc ggccgagatt gcgcacccgc
    14161 gtgatcagcg cgatgtcatc cacgggcttg gtcaaaaagt cgtcggcacc tgcctctagc
    14221 ccctggatct tgtcagagga ctggtcgagc gccgtcacca tcacgacggg gatatgctgg
    14281 gtcgccgggt tcgacttcag gcgcctgcag acctcgaacc cgtccatgcc cggcatcatc
    14341 acgtccagca gcacgacgtc gacacgctct cggctgcaca cctcgagcgc ctcccgcccg
    14401 gagcaggccg tgaggacgtc gaagtactcg gccgacagcc gcgcctcgag cagcttcaca
    14461 ttggctggaa tgtcgtcgac caccagaacg cgagcagtca tggtttaccc tacgcactac
    14521 tactgggcag acgcgattct ccaatgaacc ggcggatcgt gtcgatgaag ccactgacgg
    14581 aaatgggttt ggagatgtag gcctcgcatc cgccctcgcg aatgcgctcc tcgtcccctt
    14641 tcatggcgaa tgccgtgacg gctatgatcg ggatatcccg cagatcgtcg tcgtccttga
    14701 gccacttcgt gacctccagc cccgagacct cggggagctg gatgtccatg atgatcaggt
    14761 ccgggcggtg ctcgcgcgcg agatggagcg cttccatccc gtgccgcgtt tggagggttg
    14821 catagccgtg agcgtcgagc agatcgtgga agagcttcat gttgagctca ttgtcctcca
    14881 cgatcaggac aagcttcgct gatcggcgca tgagggttcg caggccattg atggtcgccc
    14941 ctcgatcctg ataattcatg gcgttggcca gcctgctctt cacccagccc actgttgcac
    15001 cgattcgaag aacccgcagc aggcgcaatg tgccccggga gccgtaacca acagtttaag
    15061 atatacagtc acggaaaatt aaatctccga agcgagcgaa gagcctgccg cgatgcgatc
    15121 cgcacctcag atgccccttg aagaagccga ggtgatcggc ctcaaggcct tggctttcct
    15181 ggccgaggac gaggcgcggc tgagccggtt tctggcgttg acgggcctga ggggggatga
    15241 gctcagagcg agggccgccg acgcggcgac gctatgggcc gttctgaatc atctggctgg
    15301 cgatgaatcg atgctcctcg ttttcgcagc ctcggcctcg ctgccgccgg agcggatcgg
    15361 gccggccttg cagctgcttg ccagccaggc ggacggcaca tgacggccaa tctgcaggag
    15421 cgcatccgga tcggcatcga cctcggcggc accaagattg ccgcggcggc gctgctgccg
    15481 gatgggcaaa tgaccgagga gctccggctg ccgaccccgc ggaacgacta tgaagcgacg
    15541 ctggaatcca ttcgccagct agtggagcag ttggaggcga acacggggga acgcggaacc
    15601 gtgggtgtcg gaatgccggg ctcgatctcg cctgcgaccg ggctcgtgca gaattccaac
    15661 tcgacctggc tcaacatgca accgttcgac cgtgacctct cccagcggct cgggcggcgg
    15721 gttcgtcttg ccaacgatgc gaactgcttc gcgctgtcgg aagcaacgga cggagcggct
    15781 gcgggagcgc gcaccgtctt cggcgtcatt cttggcaccg gctgcggcgg cgggatcgtc
    15841 gtcaacggtg agctgctgaa cggcccccgc ggcattgccg gcgagtgggg ccacaatccc
    15901 ctgccctggc cggagccgga cgagtacccc gggccgcact gctggtgcgg gcggcgaggt
    15961 tgcatcgaaa agtggctctc gggccccgct ctggaagagg atcaccatcg cgtcacaggc
    16021 gagcggctga ccgccgtcga gatcggccgt cgcgccgccg cgggcgattc agcaagccgc
    16081 gatacgctcg cgcgccacgc atcgcgcctg gcgcggggct tggcccatgt ggtgaacatc
    16141 ctcgacccgg atgtcatcgt tttgggcggc ggtctctctc agttgaaaca tctttacgac
    16201 gtggttccga ggctgatggc agaccacgtg ctcgctcaca acgcggcggt ggacatccgg
    16261 cccccgaaat ggggcgatgc cagcggcgtg cgcggcgccg cgtggctctg gggtcacgcg
    16321 agatagtctc ccgattccga agttccccac tcctaggcgg tcacgctcgg cggctccgag
    16381 atatgtgacg aaatcaattt gtcttccgtc cgctgctctg gctggatgcg catggctcgg
    16441 gctccagtat cgtgagctcc ttcagctcac cccggatgga gaaattccca taatcgatca
    16501 ggaggcgccg tgacacgccg ttgtcgaaat agatgaagga cagctcatag gccggcaaag
    16561 cgtcctcctg ctcggttccc ttttcgtagt agctcagagc cacgggccaa gcggggagct
    16621 cgttcaaaac gtcggcaccc ggaacttcgg gaagttgctt gttgtagccc ggtgggaggc
    16681 gggagccgat ataggcgttc gtcgcgtaga ccttctcgcc tttttcggag gcatcgaaca
    16741 gatcggtggt gaagatatgc ttgccctcgc gggccgcctc cagcatccgg ctcgagtgct
    16801 ggatcgggaa aatggctgca ttgggcagct ttatggtttt cttcttgggc tgcttcagct
    16861 cgacccggat ttccgtcttc ctgcgcacgg cttcgccgac ggtctgctcg acgcgctgct
    16921 ggttgcggta ttgactgttc tcgaagcgaa agcggtcacc ggctgcatcc tcccagctcg
    16981 aggtgcgcat gtcggtgatg ctcgtttcgc cctcctggtt cgtcgtgctg gtaacgaacc
    17041 gcatcttctg cgtgaaaccg ctgcaggccg ccccggtgaa ctcgtagacc attcggcccg
    17101 tgagctcgga aatcccggca cccggccgcg catccacgag tgtgatgtcg tacacggcgc
    17161 gatgcggcgt aagcccgatc gcttccgctg ctcctgcggg cacaaaagcg gccagtgcgc
    17221 tccatgcaag ccctgctgcg accagtctcg gtccaatgcc catcaagcct ctccagcgag
    17281 gatccgccgc acatctcgcc tgggccggaa gcctcacacg gtcgatgcgg cttgcaaagt
    17341 cagcactctc acgtctacca gaatgctgtt tcctgcaacg taaacgatct tgggccaagc
    17401 gatgttcgag cgcggcggtg tttccctcgg cagaggcttc ctgcggccga tcgcccatcg
    17461 cggcctgcac gacgcagccg agggaattat cgagaacacg gccccggcct tcgaagcggc
    17521 gatttcccgc ggcttcggta tcgagtgcga cttgcggccc gcgagcgatg gcacaccgtt
    17581 cgtgttccac gattcgacac tcgacagact gatcgatgcg gagggcccta ttgcggccag
    17641 ggaaccggcc cagctctctc gactttgcta ccgcggccag agcactcgca tcatctctta
    17701 tagcgacctg ctcgacctgg ttgccggaaa tgtgccgctg ctggtcgaga tcaagagcga
    17761 ttgggatgag ccgaacccgg cctttctctc ggccgttgca gagctctcca tggcctacag
    17821 gggcgcgctc gccctcatgt ccttcgatcc ggccgtcatc accgcattgc ggcatctggc
    17881 acccaccatt ccgcgtggca tcgtttccgg ttcttacgaa gggccaggct ggtgccgcga
    17941 cagaatagac gccgagcgtg cctatcgcct cagtcactgc ctcgaggcag gccctgccga
    18001 gccgagcttc atcgcttacc atgtcgacga cctgcccacg cccgttacac gctttgcacg
    18061 cgaggtgata cggctgcccg tctttgcgtg gaccgtccgc acggccgcac agctccggca
    18121 cgcggcggcc tgggcagatg caccgatctt cgaaggctgt gatccctgcc cggcataacc
    18181 ggcgcttgtg cttgtactct tgatcatgaa aaccaatgga ttaagtcagg tttgcgaatt
    18241 gcccgtgggg acgacctcac agttaagggg cctcggcagg gacgaccctg ggtcattcgg
    18301 gagggccgca tggcttgggt agtcctcttt atagcggccg ggctggaagt cgcatgggcc
    18361 atcggcttga agtacactca cggcttcacg cgattatggc catccgttct cacactactg
    18421 gcgatggcta caagcatgta cctgctggcg atcgcggcca gaacgctgcc catcggcacg
    18481 gcctacgccg tgtgggtcgg catcggcgcg accgggacgg caatcctcgg catggttctt
    18541 ttctctgaac ccgcgaatgc ggcgcggatc gcctgccttg ggctcatcat cgccggagtc
    18601 ctgggtttac gtctcctcga aggcggctga agcaaaaggc cgcgctcggc agccgtgccg
    18661 tcgacctggc cgggcggcct gatatgctga gcgaacggag cgcttccgga gcaatgatga
    18721 aacgggacga ggctgcagtc gtgcgtgtca ccacctcgat cgccgagatc gatgggaacg
    18781 cctgggacgc gtgcgccaac ccggatgcca aaggcttcaa cccgttagtc agtcacgcct
    18841 tcctgaaggc actggaagag gcaggcacag tcgggcgccg cgccggctgg ctgcctcagc
    18901 atctcgtgct cgaaggaccg gctggccaca tcgccgcctg catgcccctc tatctcaaga
    18961 gccacagccg cggagagtac gtcttcgact ggagctgggc cgatgcctac gagcgggccg
    19021 gcggcagcta ttacccgaag ctgcagagcg cagtaccgtt tacgcccgtg cctgggccgc
    19081 gcctgctcgt tccccctggt cccgaccggg acgagcgcga gcggacactg gccctggccg
    19141 ccatcgcatt ggccgagcgc ctgagagtat catcgctgca catcaccttc ccgaccgagg
    19201 gcgagtggcg gcgcttaccg gagctgggct tcctgcaacg gaccgaccag cagttccact
    19261 ggcataacga aggctacagg tccttcgagg atttcttggc gagcctcgct tcacgcaaac
    19321 gcaaggccat ccgcaaggag cgcgcccagg ccctcgcgcc aggcatctcg gtcgagctcc
    19381 tgaccggcag cgacatccac gagaggcact gggacgcgtt cttcgcgttc tacatggaca
    19441 cgggatcgcg caaatggggc cggccatacc tcaatcgccg gttcttttcg ctcctgggcg
    19501 aagccatggc cgaccgctgc cttctcgtca tggcgaagcg gaatgggcgc tacgtcgccg
    19561 gcgcgctgaa tttgatcgga ggtgactgcc tctatggccg ctactggggc gccatcgagc
    19621 atcatccgtg cctgcacttc gagatctgct actaccaggc gatcgacttc gccatcgccc
    19681 gtggccttcc ccgcatcgag gcgggcgccc agggcgagca caaactggcg cgcggctaca
    19741 tgcccgtgcc gacctactca ttgcactgga tcgccgatcg caacttccgc cgggctgtcg
    19801 cgcgctacct cgaggaggaa cgccgggaga tggccgagca tcgcgagctg ttggccgcct
    19861 atggccccta ccggaaagcc gcggccgcca gcgaggaaac gttctgatgt ccaacaggcg
    19921 atgaagagga ccgtgcatga cctacgatga caacaacgtg ttcgcgaaga tcctgagagg
    19981 cgagttgccg gcgcacaagg tctacgagga cgagcatacg ctcgccttca tggacatcat
    20041 gccccgctgc aaagggcaca cgctcgtcat tccgaagacc cctgcgcgta acatcctcga
    20101 cgccacgccc gagcagctcg ccacctgcat gattacggtc cagcgcctcg ccgctgcggt
    20161 catgaaggcg ttcgacgcaa ccggagtcac gatccagcag ttcaatgaac ctgcgggcgg
    20221 acaggtcgtt ttccaccttc acttccatgt gctgccccgc tgggaaggcg ttcacctcgg
    20281 gccgcatacg ggcaagatgg aggataacga agtgcttgcc gcgaacgcgg agaagatccg
    20341 gcaggccctt gcttcgtaat ccgatttgac gttcgcgctc cgcgccggaa ggcgcggcag
    20401 cgcgccccag ggggcggcaa ggcacactcg gaaggagatg cctatggcgt gcatcctggt
    20461 catcggagcc agccggggta ccgggctcga gacagtgcgc aaggcgctcg cggccgggca
    20521 tcatgtccgg gcgctcgcca ggtcggcggc gagcattccg atcgtggacg agcggctcga
    20581 gaagattacc ggcgatgcaa ccgatagaac ggcactgctc aatgcgctcg agggcatcga
    20641 cgcggtcatc gtgacgctcg gcgtttcgcc aagcctcagc accagcctgt cgggcacgcg
    20701 cctgttttcg acggccaccc gcgccctggt cgacacgatg aaggaaaagg gcgtgcgccg
    20761 tctcgtcgcc atcaccggca tcggcgccgg cgaaagccgc gggcatggcg gccccctcta
    20821 cgactgggtg atcttcccgc tcatgctcaa gcgcgtctat gacgacaagg acatgcagga
    20881 gcagatcatc cgccggagcg gcctcgactg gaccatcgtc cgccccggcc tgctgaccaa
    20941 cggacctgaa accggccgct atcgcgccct gctcgacccg gcccagtggc gcgccgacag
    21001 catcagccga gcggacgtgg cggattttct ggcaaaagag gtcacagaac ggaagtacgt
    21061 ggggcaagca cccgttctgg tggggtgatt tccgagcgaa tggcgaaata gcgaatggca
    21121 aataggggct tcctcattcc ctatccgcta ctcactattc gctattcgcc ctcagcaaat
    21181 caatccggct tccgcggcac cgtcgggaca gtgccggtgg atttccggtc gcgcttctcc
    21241 tttggccccg gcagcgcctt gcgcggtacc gccgcctcga tgagggcctt ctcggcctca
    21301 tcctcgagat cctcgtcctc ctcgtcctcg tccttggcct tcggaggctt gggctcgccg
    21361 gggatgatct cgaagtcgag tttctggtcg gcaccttcgc cggtaacgag caccttcacg
    21421 gtgccgccgt gctccagctt gccgaacagc agctcgtctg ccagcggctt cttgatgtac
    21481 tcctggatca cgcgggccag tggacgggcg ccgaacttgt cgtcgtagcc cttctcggcc
    21541 agccacttgg tcgccgcctc ggacagctcg atggtgacgc cgcgatccgc gagctgcgcc
    21601 tcgagctgga agatgaactt ctccaccacc ttgaagatga tctccggcgg cagacccgag
    21661 aacggaatga ccgcgtccag acggttgcgg aactcaggcg tgaacagctt gttgatcgcc
    21721 tcctggtcct cgccctcgcg cctcgcccgg ttgaagccca tcggcgcctt ggccagatcg
    21781 ctggcgcccg cattggtcgt catgatcagg atgacgttgc ggaagtcgac ctgcttgccg
    21841 ttgtggtcgg tgagcttgcc gtggtccatc acctgcagga ggatgttgaa gagatcggga
    21901 tgcgccttct cgatctcgtc cagcagcagg acgcaatggg gatgctgatc aacgccatcg
    21961 gtgagcagac cgccctggtc gaaaccgaca tagcccgggg gcgcaccgat gagccgcgag
    22021 accgtgtgct tctccatgta ctccgacatg tcgaagcgca gcagctcgac gcccatgagg
    22081 ttcgcgagtt gcttggcaac ctcggttttg ccgacacccg tcgggcctga gaacaggtag
    22141 cagccaatcg gcttctccgg ctccctgagc cccgcacggg cgagcttgat ggccgaggcc
    22201 aacgcctcga tggcgcggtc ctggccgaac accagccgct tcagatcgcg ctcgagattg
    22261 ctcagcacct cggcgtcgct cttggtgatc gtcttcggcg ggatgcgcgc catcgtggcg
    22321 acggtcgcct ccacctcctt gacggtgatc ttcttcttgc gcttgccctc cggcaccagc
    22381 atctgcgagg cgcccgtctc gtcgatcacg tcgatcgcct tgtcgggcag cttgcggtcg
    22441 tggatgtact tagccgacaa ctccaccgcg gccttgatgg cctcgttggt gtacttgagt
    22501 ttgtggtact cctcgaagta cggcttcagg cccttcagga tctcgatcga gtcctcgatc
    22561 gtcggctcct tcacgtcgat cttctggaag cgacggacca gcgctcgatc cttttcgaag
    22621 tgttgccggt actccttata ggtggtcgac ccgatgcaac gcactgtgcc cgactgcagg
    22681 gcgggcttta gcaggttcga tgcatccatg gcgccgcctg aggtggcgcc cgcaccaatc
    22741 acggtgtgga tctcgtcgat gaagaggatc gcaccggggt agttctcgat ctccttcatg
    22801 acggccttga gccgctcctc gaagtcgccc cggtagcggg tgccagcgag cagagcgccc
    22861 atgtcgagcg caaagatcgt cgcgttcttg agaacgtccg gcacctcgcc gcggatgatc
    22921 ttgcgggcga ggccctcggc gatcgccgtc ttgccgacgc ccggatcacc gacgaacagc
    22981 ggattgttct tctgccggcg gcagagcacc tggatcgtcc gcagcacctc ggcctcgcgg
    23041 ccgatgagcg gatcgatgcg gccctcgcgc gccttcttgt tcaggttgac gcagtaggtg
    23101 ttgagcgcat cggcccccgg cttcttgccg tcgccctcgg tcgtgtcctc gtcgacgcca
    23161 cgcgccgggc gcggatcgga catgccaggc cgcttggcga tgccatggct aatgtactgg
    23221 accgcgtcga accgcgtcat ctcctgctcc tgcaggaaga aggcggcatg gctctcgcgc
    23281 tcggcaaaga tggcaacgag cacattcgcc cccgtgacct cctcgcgtcc cgacgactgc
    23341 acgtgcacca cggcgcggtg gatcacgcgc tggaagccgg tggtcggctg cgcttcggat
    23401 ggtccgctca cctgcagcga gccgagctcg gtatccagat attcagtgac gcgattgcgc
    23461 agcgcgtcga tgtcgacgtt gcaggcccgc atcacgccgg ccgcgtcgcg gtcgtcgatc
    23521 agcgcgagca gaaggtgttc gagcgtggcg tactcgtgct tgcgctcgtt ggcgtattcc
    23581 agcgcccggt gaagcgctgc ttcgagactc tgtgagaacg atggcaaggg ggagctactc
    23641 cttctccatg gtgcattgaa gaggatggcc atgctgacgc gagaaatcca tgacctgcgt
    23701 caccttggtt tctgccactt catacgtata gacaccgcac atcccgacgc ccttgtggtg
    23761 gacgtgcagc ataatgcgtg tcgcttcttc ccgtccctta ttgaagaacc tctcgagcac
    23821 atgcacgacg aactccatcg gcgtgtaatc gtcgttgagc agcaacacct tgtacaggtg
    23881 cggccgcttc gtcttgggcc gggttttcgt aacgagacca gtgcgaccgt cttcctcgct
    23941 gcctctgtcc ggcccgttgt tccctgccat cgccatcaat ttcgtcatct gctccacacg
    24001 ctccgcgtct gaagcgtccc tacctagctc gccaatcaag tttccaagtt ccaggatttc
    24061 aagttacgca acctttgcgg cgcggcaacc cccggatcgc gaacattata cgcgatcttc
    24121 agccgaaacg atccactcgc ttgcaatcaa tcagcgccga tcggacgcaa aaaaggcccg
    24181 gtccacatgc ccgagcctgc cagccgttcg gcccagcact acggctgttc cacctggggt
    24241 gctcaccgac ccgtgtcaag gggcttgtaa gcctccttgg cgaggttggc atacatcccg
    24301 ccgagcttcg tcatctgctg catgtactcg tcgtacacgc gtctggcata gctggtctgt
    24361 atctcgatgg cctgctccag gctcttggcg gccagcagct tctcgaacgt ctttgtgctc
    24421 tgctcgaatg agcgcttgct gtaatcagtc atttcggcag caattgcctg ccagttcttg
    24481 ttccagtctc ccaacaggcg cattgcacga tccaggtttt cctggccgag cttttgcacg
    24541 tccacactgt tcatcatgac gcaatcctgt gtgcgagcgg ggctgcggag gggcagcttc
    24601 gcgagtttgc gctgcacaac atacgcctgt gagctaccac gtcaagtcag ccagtccctc
    24661 gaaattcaac cgtggctggt gccgccattc cgacgttcgc ccgagcccgt atcatccaag
    24721 cgtcggccat taaccagatt ctagtccccg cctcgtagag ttcgagcaac ccaaaggcgc
    24781 actgcttggg gggccgtcgg aacgccgcaa aggtgcccca aaccgctgtt tcggtgggaa
    24841 cgggtcagca actggggcgc ggccgggggg gtgtggcagt caccaatacc ccgccaacgc
    24901 tttcgagcgc cggagcggcc gcgaaggccc ggcatcgtgt ggtgagtaat tgatgcggct
    24961 cgacacattc aatctcgacc tgcggcgcct gcacgtcggt ttctgtctgc tcgccgcaat
    25021 gctcgtcgcg ttgtcctggc ctggtgaggc ttcggccagt aaccgccggg cctcgatggt
    25081 gatcgacgcc aacaccggca aggtgctgca cgcctccaat gcggacgcgc cgcgccatcc
    25141 agcctccctc acgaagatga tgacccttta cctcgttttc gaggagatcg agagcggccg
    25201 tctttcgctc gattccaaga tcaagatgac gaagtggggc gcgatggcgc cgccctccaa
    25261 gctcggtctt aaggaaggcg aggagatctc cgtccgcgac gcgatacggg ccctggtgac
    25321 caagtcggcc aatgacgtcg cgagggcgat tggcgagcac atcggcggga ccgaggtgaa
    25381 cttcgccaag atgatgaccg agaaggcgcg cctgctcggc atgtcgaata cgacgtttcg
    25441 caatgcatcc ggcctgccgg acgtcgagca ggtgacgacc gcgcgggaca tgctgacgct
    25501 ggcgatgcat ctgcaggacg atttcccgca gcattatcac ttcttctcga cgcgcgtctt
    25561 caatttccgt ggccggtcgt atcgcaacca caatgcgctg ctccggacct accctggaac
    25621 ggacggactg aagaccggct acatccgcat gtcgggcttc aacgtggtga cctccgtgcg
    25681 gcggggcggc cggcacgtgg tcgccgcagt gttcggcggc aagaccgcgg caagccgcga
    25741 tgccgagatg cgcgcgctgc tcaaccgcac gatctccaag ggctcgacca agaagacccg
    25801 caagccggac gcagtctcgt tctatcgcgc gacaccgcgg gctccggcga agcggcccga
    25861 gcctgcagcg gagccgaccg tcgtggcagc gagcaccgct cctccggttc caacccgagc
    25921 ggcggaggct ccacccgttt ccgccgactc cacctccaag gcaccgacga tctccgtcgc
    25981 gaaggtgcgg tcggtcccgg tggcgccgat ggcggctcag gagaacgagc cagtcctcgt
    26041 acaggcatcg gctggacgtg tcgcgacgag cagaccacct ggagcgatgg cgcttggccg
    26101 caaaccatcc acgcttgatg cccaggccga agccctgcag caggagcagc cggcggtcga
    26161 acgcgtcgcc ttcgtcgatg cttcggccct gtcgcctgct ccctctccgg ctccagtgac
    26221 ccgggcggcc ccttcagccg aagcggcacc aaagacggct tcgcaggaag cgtccaagtc
    26281 agcccgtgag ggcagctacg tgttgcaggt cggcgcctac tcgacggccg ccgaggcgaa
    26341 gaaggccctc gttgcgacgc tggaacgcgc tcccgccctg ctcgccgaag cctccccggt
    26401 gaccgttccg gtcaaggccg gcgccaagca gctctaccgc gcgcggttca caggtttcga
    26461 ccccgccgcg gcacgaagca cctgtgagga gctgcgccgc cggagcatcg actgcttcgt
    26521 caccaaagcc gactgatacg cgccagtgct caacgcccga gcagtatgtc ttttgccaga
    26581 ttgattttcg ccgcgagata gctcgagcca ccgacatcgg ggtgcagctt cagcatcagc
    26641 tcccggtgtg cccgccggat ctcctcctcg ctggcgccag gcgcgaggcc cagaacgtcg
    26701 taagcctgct cgcgcgtcat gtcctcgcca cccgcggcag ccgagccggc gcgagccatg
    26761 tcatcccgcc atgaggggta gacgctgtcg agataggcct cgacgatctg cgccgactgc
    26821 atgtcgctga accggcactc ctgccacagc atcgccagct ccgccggtgc cagggtttcg
    26881 aactcccgtc ccgcaaatgt gcccttcaga acgcggccgc gcatggcgcc ggtatcgtgc
    26941 tcgagctcca cctccagata gtccgtcacc acccgcgagc tctggcccgg gctcttgttg
    27001 ggctggcgca atggcgcgcc gccgcctccg aagagcatcc aggttccgaa cgctgcgagc
    27061 gggcccgccc aggacagcag ccctcgcaag gccagcgccg cggcggcggc gagcgccaca
    27121 acgcctgtga cgatgcggat ctggcgcgcc atggcgctga cgttggctcg cgtgaacccg
    27181 cgcagcgcca gcaggaccaa gagcaacgcc gccaggccga gcaggaagta ctgcatgccc
    27241 cctgttctct ctcacttcat ctgcgcaagc agcagctgtg cgccccggcc agcgctccga
    27301 tggctgagcc gctcgagcgc catccggcca ccagccgcat agacggcaac cgccgaaagc
    27361 agctcgcgga gctgcgcagc cgatcccgcg tcgaaccggc agtaggcgcc gcgcgtcagc
    27421 cgggcaattt cacggaaggc ccgttcggcg cgcgcatcat ggccctcctg aaacaggaac
    27481 accggcacgc ccagtaaggc cagctcgccc gcgcggccgc acaggtcatc gatgctctcc
    27541 tccatgcagt cgccgacgta gacgagcgcg ttgatcttcc gccgctcggt ctcttggcgc
    27601 gcatgcgaga ggaccttgcg gatctgcgtg tagccaccct ggcaggaaac cgtggtcatc
    27661 agccgggcca gcgcgtcggg gtccgacacc catttcgacg cccggcattc gccggcaccg
    27721 cggaagtaga cgagttgtac gtcgagcccg ccaacctcct tgaccgcctt gaacatgtcg
    27781 gcctgcaagc ccagcgccat gtcccaggtc ggctggcggc tcatggtggc atccatggcg
    27841 aagacgagcc ggccgcgtgc ggttgcgtgc ggcgtcatcg ccttcatgcg ggagatgaac
    27901 tgggccacat cccgatccga ggagcggctc gccggcgtgg tgtcctggcg ggctttgtcg
    27961 agaggcgcgc ccgatcccct cgtcgtaaac ggctccgtca cggacccgct ctccgttgca
    28021 ccagacgtcc tgcttccctt gcccatggcc taccctaact ccacgcgcac cggccgacag
    28081 attagccgga tccggcgctt aaccggctat tgacaggtgg ccttccggca ggctccgcgc
    28141 aataccaatc cccgcccgcc cacagagcct cactgccgcg gcgtggcgag agaggtgtcg
    28201 agaccgagcc ccagaagctg cacgttgccg ccagtcgcgg tgatgtcggt gctgcggact
    28261 cgctccgtcg caaagcgcgg cagatagtgc ggcccgccgg ccttggggcc ggtaccggac
    28321 agcccctcgc cgccaaacgg ctgcacaccc accacggcgc cgatctggtt gcgattgacg
    28381 tagagattgc cgacgcgggc gcgctcggca acgaaatcgg caacggcctc gatgcggctg
    28441 tgcaggccca gcgtcagacc gtagccagtc gcgttgatgg catcgatcac ctcccccagg
    28501 tgcccctgct ggtagcggat cacgtgcagg attggtccga acacctcgcg ttccagacgg
    28561 gcgatgtggt cgatctcgaa aaccgccgga gtgacgtagg tgccgacccg gcatttttcc
    28621 ggcagcttca gatccgccag caccttggcc tcgcggtaca tgcgcgcctt gtgcgcgtcg
    28681 agcgtgtcct gggccgtctc atcgataacg gggcccaaat ccgttccgta gtcgaacgga
    28741 tcgccgacat cgagctcctc gacggcgccg accagcatgt cgatcacgcg cttggcgacg
    28801 tcctcctgga cgaacagcac ccgggccgcc gagcagcgct gcccggcact gtcgaacgcc
    28861 gagcggacgg catcccgcac cacctgctcc ggcaaggcgc tggaatcgac gatcatggcg
    28921 ttcatgccgc ccgtctcggc gatgaacggc acgatggcgg cgcgccggtc ggccagcgcc
    28981 cgctggatgt gccaacccgt ctcgttggag ccggtgaaag ccacaccctg gatgcgcgtg
    29041 tccttgacga gcatggcgcc gatgtcgcct gcgcccggca acagatgcag cacatcgggc
    29101 ggcacgccgg cctcgtgcag gagcctggtg gtgaagtagg cggtcatggg cgtctgctcg
    29161 gccggtttcg ccagcacagg gttgccggcg gccaaagctg cggaaacctg ccccgtgaag
    29221 atggcaatcg gaaagttcca gggactgatg caggcgaacg ggccacggcc cctcagctcc
    29281 agcgtgttcc gctcgccggt cggcccatga agctccgtcg gccccgagaa cagccgccgc
    29341 gcctcgctcg cgtagtagcg cagaaaatcc actgcctcgc ggatgtcggc ctgggcgttc
    29401 tcgagggtct tgccggcttc ggccacgatc accgccatga gccgtgcccg gtcccgctcg
    29461 agcaggtccg cagccttctc aagaatttcg gcacgtgccg gtccaccgcg cagatcccag
    29521 tcgcgcgcgg ccgcacaggc gcgggtgatc gcctcctcga ccatgtcggc gtccgcgaga
    29581 cgcaccgtcc caagctcgcg gcgatgatca tgcggacagg ttgcaatcag ctcctcgccg
    29641 ccggcgggcg tctcgccgcc gatgatcggg ccgaccgcca gcggctgctg gagcgcttcg
    29701 gccatttcgg cctgcagggc cgaccgtacc gtcggctcgc tcagcggcag gccgagactg
    29761 ttgcgccggg cgggaccgta gatttcgggc ggccgcggca gcctggacgg ccgcgcctct
    29821 ggcggcagct cccgctcgct ctcggccatc tcgaccggat cgcggatgat atcggcgacc
    29881 ggagcctcct cgtccgccag ccggttcacg aaagaggtat tggcgccgtt ctcgagcagc
    29941 cggcgcacca gataagcgag caggtcctcg tgtccgccga ccggcgcgta cacgcggcac
    30001 ggccggttga gcttgccccg cccgacgacc tcctcgtaaa gcgcctcgcc catgccgtgc
    30061 aggcgctgga attcgtacgg cgcatcgccg gccgacacga aggccgccgc gaccgtgtgc
    30121 gcattatgcg tggcgaattg cggatagaac gcctgcggat cggagagcag aagccgcatg
    30181 caggcgagat atgaaacgtc ggtatgcagc ttgcgggtga agaccggata gtcggcaaac
    30241 cccctttcct gtgcccactt gatctcgctg tcccaatagg cgcccttcac gagccgtacc
    30301 ggcagacgct tgccggtacg gagcgccagc cgtctcagcc agcggagaac cggaatggcg
    30361 cgcttgccgt aggcctgcac ggcaaggccc agcccgtgcc agccatcgag cttcggatcg
    30421 gccagcaccc gcgcgaacag ctcgagggac aggtcgagcc ggtcctgctc ctcggcatcg
    30481 atcgtgaccg cgatgccgcg tgcccgtgcc gccgccgcca gctcgacgat ccgcggaagg
    30541 agctcgcgtt cgagccgcgc ctccttgccc ggctcgtagc gtggatggat tgcagagagc
    30601 ttgaccgaaa ggctgggccg cgccatcagc gcatcggggt gctcggaaac gagcggcccg
    30661 gcatgccggc ccacctcgtc gagcgcaagc atgtatctct cgaaatagcg ctccgcatcc
    30721 cgtgccgttt tcgccgcttc gcccagcatg tcgtaagaga agcgatagcc gcgcgcctcc
    30781 atcggcgcag cgcgccccag ggcttccttg atggtccggc cgagcacgaa ctggttgccc
    30841 aggatacgca tggcgtgccg cagcgcctgc cggatcaccg gctcgccgga gcgcaccacc
    30901 atgcgcttca gcaggttggt cgggcgctcg ctctttgcgt cccggagctt gacgatctgc
    30961 ccggtcagca tcagccccca ggtcgaggcg ttgacgaaca ggctgtccga ccggccgaga
    31021 tgcttctccc actggccgcc accgatcttc tcggcaatca gcttgtcggc ggtctcggtg
    31081 tctggaatgc gcagcagtgc ctccgcgagg cacatgagga tgacgccttc ctcgctggtc
    31141 agcccgtatt ccagcatgaa ggcatcgatc cccgagtatt tgtgccgtcc cgcgcgcgcc
    31201 gcgtgaacga agcgccgcgc caggtcgccg atgcgccgcc gctcgtcctc ggcgaagatg
    31261 gcccgctcga tcagagcccc caccagcctc tgctcatcca tgaggtgata ggcggcgatc
    31321 tcgtctcgcg gcggaatcac ctgttccgca tcgcgcttgc ccgagtgcag tgctatgagc
    31381 tggttggcca tgtcggtcgt ctcatcggac tcacattgct atttatttag cagattgcag
    31441 acccggctcc gatcaattcc gccgcgcctc gatgcgtcgg tggcctgctg caagatggct
    31501 gctatcgcga agaggccctt gacgaacaag ccatcccgca cgacgttgcg cgccgcaaca
    31561 taaggctgcg aaagatgggg taggtccggt gtcgccagga caggtcatgg tcgttcgctc
    31621 cctcgcccca ctgcgcgagg tcgtcggtgg ctggcgcagc ggcggcgagc gcatcggact
    31681 cgtgccgacg atgggcgcct tgcacgaggg ccacctcgcc ctggtgcgca ccgcgaaggc
    31741 ccgtgccggg cgggtggtgg tgtcgatttt cgtgaaccct acccagttcg cgccacacga
    31801 ggatttcacc acctatccca gggacgaggc cggcgatctc gaaaagctgg ccgcaatcgg
    31861 ctgcgacctc gtctgggcgc cagacgtgaa cgtaatgtat ccccccggct ttgccaccag
    31921 catcgtgccg aaggggccgg cggagggcct ggagaccgat catcggccca atttcttcgt
    31981 gggggtcgcc acggtctgca ccaagctgtt cacccaggtc gcgcccgatc tcgccgtgtt
    32041 cggggaaaag gactatcagc agctctgtgt catccggcag gtggtaaagg atctcgacct
    32101 gcccctggag atcgtcgccc accccacggt gcgcgagccg gacggcctcg cgatgagctc
    32161 gcgcaatgcc tatatgactg ccgaggagcg ggcacgcgcg ccgataatac ataaggtgct
    32221 gctgcagacg gcagaagcgg ttgccagcgg gcagggcata gccgcggcca cgagctgtgg
    32281 acgtgaggag cttgtggccg ccggcttcaa ggtcgactac ctggaagtgc gcgacgcaga
    32341 gacgcttgct cctgtcgagg acgggtcgaa acggccgctt cgggtgctcg ccgccgcttg
    32401 gctcggcaag acgcggctga tcgacaacgt ggcggtcggc taaccgaacc caaataccac
    32461 accgggattg ccgggatcgc ttcgaagcta ttccgcgacc ctggcgaaac ttgccaagcc
    32521 acggcggctt gtgcgaggca actgcccggt atccagcgtg agattcgccg gcacgggtcc
    32581 cggaaataca ctcgtgccgg gcgtgtgctt cgtccgctcg agcgaggtcc aacgcgcatg
    32641 gcccagtctc aacgcgatct gttcgcaagg cttgtagacg aaatgaacca caagacggcg
    32701 cgcgcccgca gggtcatcga cgcctcagac acctatacgg ctgcagatat cgaggtcctg
    32761 gaggggctcg agcctgtccg ccggcgcccc ggcatgtata tcggcggcac cgacgagagg
    32821 gcgctgcatc acctgttcgc cgaggtcatc gacaacgcca tggacgaggc ggtggcgggt
    32881 catgcgagct ggatcgacgt gcatctcgat gcggacggct ttctgacggt gacggacaac
    32941 ggccgcggca ttcccgtcga cccgcatccc aagttcaagg acaagtccgc cctcgaggtc
    33001 atcatgacca cgctgcactc gggcggcaaa ttcgagagca aggcctacaa cacctcgggc
    33061 ggactgcacg gtgtcggcgt ttccgtcgtc aacgcgctct cagagacttt ggaggtcgag
    33121 gtcgcgcgcg gccagcagct ctaccgcatg gtgttcagcc gcggcgagcc gcaaggcaag
    33181 ttgcagaggc ttggctcgat catgaaccgg cgcggcacga aggtgcgctt caagccggac
    33241 gagaagatct tcggcaaagg cgtggccttc aagccggcgc ggctcttccg catggcgcgc
    33301 tcgaaagcct acctgttcgg cggcgtcgag atccgctggt cgtgcgaccc gtccctgatc
    33361 aaggacgaca caccggccga ggcggtgttt cacttccccg gcgggctcaa ggactacatg
    33421 ctcgccacca tcaacggccg cacgctcgtg accaaggaca tgttcacggg ctgcgtcgag
    33481 aaggaaggcc ggcacggctc cgtcgagtgg gccatcgcct ggatggccga cgaggacggc
    33541 ttttccagct cgtactgcaa caccatcccg acacccgaag gcggaacgca cgagcagggc
    33601 ctgcgccagg cgctgaccaa gggcctccgc gcctacggtg agctcgtcgg caaccgccgc
    33661 gcggcacaga tcaccgccga tgacgtgatg agcacggccg cctcgatgct ttccgtgttc
    33721 atccgtgagc cagaattcca gggccagacg aaggacaagc tcgccacgca ggaggccgcc
    33781 cgcatcgtcg agaccacggt gcgcgacgcc ttcgaccact ggctcgccga atcgccggcg
    33841 caagccaccc gcctgctcga gtggaccatc gagcaggcgg agcagcgcct gaagcggaag
    33901 cgcgagaagg aggtcagccg gcagagcgcg acacgcaagc tgcgcctgcc cggcaagctc
    33961 gccgactgca cgatccagtc cgcaaaaggc accgagattt tcatcgtcga gggcgactcg
    34021 gccggcggct cggcgaaaag cgcgcgcgag cgcgaaacgc aggccgtcct gccactccgg
    34081 ggcaagatcc tgaacgtggc caacgcctca tcggccaagc tcgcgcagaa ccagctcctt
    34141 tcggacctca tactggcgct cggcgtcggt acgggctcca agtaccggga cgaggacttg
    34201 cgctacgagc gcgtcatcgt catgaccgac gccgacgtcg acggcgcgca tatcgcgtcg
    34261 ctgctgatca cgttcttcta ccagcagatg ccgcagctca tcgacaacgg tcatctcttc
    34321 ctggctgtgc caccgctcta caagatcacg cagggcacgc agtcggccta tgcgcgtgac
    34381 gagaagcaca aggaggagct gctgcagacc gtcttcaatg gccgcggcaa gatcgagatc
    34441 agccgcttca agggcctcgg tgagatgaat gccgctcagc tcaaggaaac caccatggat
    34501 ccgaagcggc gcacccttct gcgcgtcgaa atcggtgacg gcgaccgcag cgagattgcc
    34561 gaggcggtga acaatctcat gggctcgaag ccggaggcgc gtttccgctt catccaggag
    34621 cgtgcggcgt tcgccaagga tctggatatc tgacggtgcg gaccaacgac tccctttcag
    34681 ccgaatgaga gcgcaactcg agcgcgaccg attcactgga ataagcagag gagccgggcc
    34741 tttgggcccg gctttctgat tcccgtgcag gttctcgatc tcaggtataa aggctgtgtg
    34801 tgaggctttc cggtcatagc gcaccatcgg ctgcgatgcg catactactg acgtctacga
    34861 accttcagga gacattcgtc atgagccgca tcttggctgc gctcgccgtc gccctttcgc
    34921 tgtccctcgc ctcgaccggt gtgatcgctg agaacgcgaa ggcgccggca cagaaggctt
    34981 cgacaccgaa ggtgctcaag gccggcaagg gcgagaaggt ctgccgttac cgcttcccga
    35041 acggtgagca gaagagctgg atctgcaagc aggaagagcc ctgctgcgcc tgggacctct
    35101 tttcctacgt gaagtgcggg tcgacgatca ccgggtgcct ctaagtctcc ccgtttcgca
    35161 attcgccaga actccgcgat cggaggacct tggtcttcag ggccaaggtc gccggaaagc
    35221 ccataccacc gcgtcgggga gggaggtgtg tggcggtgcg gcagcacagg ccttccggcg
    35281 agtccaacgc ccgcgccgct ttcgcgactg cgagctgatg aagggccggg cggcggagaa
    35341 gcggggtttc cccaccgccc gactcgggag ccggcatttg caaccgaacc ccgagtttcg
    35401 acttgcgacc cccctgcagg tgcgtttcga gcgaagccgc acctgcgggg ccgtcgcggc
    35461 cgtcggtcac gtggaggcac acgacaacgc tccgacagcc cgtcaactgc gccggatgac
    35521 gttacgtttt tacgtccttt ttgcagcgat accgaccgga cccggcaatc cgatcgttga
    35581 ggttaatttc gcctgccgaa gttgggctga gcatggccac ccaggggcgt aattgtggct
    35641 ggtcggatca acttgcctgt aacatactga aacgattgtg atgcagccgt cgatgcggtt
    35701 tgagatccgg tcgaccgaac tgcggcagtt cagtgtcacg ccggcgcggc gagtgatatg
    35761 actgccaagg ggagccattt cgccgctcag tacgaaaacg aggatttccc gcttggaagc
    35821 tgccatgagc tgagccaatc tgtcggccag gctcgatgcg agtggctctg atgcatcttt
    35881 cgttccatac actcgacgtc ttcacggaca ggcccttcag tggcaatccg ctcgcggttg
    35941 tcctcgatgc cgatcggctc accacggagc agatgcaatc catcgcgcgc gagttcaacc
    36001 tgtccgaaac ggttttcgtg cagaagccgg ccaaccccgc acatacggcc aaggtgcgca
    36061 tcttcacgcc gggcgcggag cttcccttcg ccggccaccc gactgcggga gccgcagtcc
    36121 tcctggccga gctgcgtgca ccggaggccg cggaccatga cgcgatcatc gtcctcgagg
    36181 aagcgatcgg catcgtacgc gtcggcgtcc ggctgcggcg cggcgaggcg agctttgccg
    36241 agttcgacac gccgaagctt ccgcaggagt cgggcagcat gccgccggtc gagcaaattg
    36301 cggctgcgct gaacctgctg ccccgtgaaa tcggcttcgc caatcaccgt ccgacctgct
    36361 ttgctgccgg caacagcttc ggcttcgtac ctgtcgcctc gctggacgcc attgcgcgcg
    36421 cggccgtgaa tagccagcac tggggagctg ccttctccaa tcgggggctc acgggcgcct
    36481 ttctctactg ccgcgacacg gtgcacacca cgtcctcctt ccatgcccgc atgttcgcac
    36541 cggacttcgg cgtgcccgaa gatccggcca ccggctcggc ggccgtcatt tttgccggtg
    36601 tcatccagcg gttcgacgcg ctgcccgacg ggcatcacaa gcgggttctg gaacagggct
    36661 tcgagatggg acgcccgagc atcatctccc tcacgctcga ggtcgccaat ggcgagcttc
    36721 acaacgtgcg aatcggcggc aatgcagtgc gggtgaccga gggccgaatt tcgacccctt
    36781 gattgcggaa gagaccgatg gatcagaaaa agcttgtgac cgcagcgatc gcacttttca
    36841 gtcttgccgg cctccagctg tcgccagcgg ctgcggaaca ggtcgaatgg ttcggcaccc
    36901 gcagcgagga tggagcatcg ctggtctacg gcacgccgaa cagcggctac ggtaagatcg
    36961 tgctgagctg cgcggccgga caggaagacc tcaactttgt ctacgagcac gagccgattg
    37021 acccgaagaa cggcgtccaa gtcgaggtgc tgctctctgc gggaggcgta gagacctcca
    37081 ttccgaccac cgggacccgg ctggaaatgg acgacgtatt catcctcgag ggccagacca
    37141 agctggacga ccgcctgcgc aagatcctga cgtcgcccgg ctccctcatt ctgacagttg
    37201 aagacggcac ggccgaattt ccgctcgacg gcgctgcaaa cgctgcccgc gatctgctcg
    37261 aggtctgctc gccggaaggg tgatcctcgg gatgggatct ctggttggcg gaatcgcatc
    37321 cgtcgcgaaa acgatcgaaa ttcgaagagc catccccctc ttgtctgcga cagaaggggg
    37381 tgatcgctgc agcggttttc acctcattcg ggctgccgag gaatattcgc tcaaatttgc
    37441 ggtgcgaacg caatacgatc ctactcgacg cgtggctgca ggccgagaag agtcagtgag
    37501 agtggcaggc ccaaaagcaa ataagggcct gccgaaagtc aatcgatcag ttgccggcga
    37561 ccttcatcaa ctctgaagca cacgcgtcgg caacctcacc attgggttcc gtagtgccgg
    37621 gcattgtcgc ccaaccttgc tcttcaacga aatcaccacg cttccaggaa ctttctccct
    37681 taagcgcggc gagctgaacc tgttgctgct gctgtgtgag gctcttgaac tgtgcgacac
    37741 aaacgggagt gagtgcggcg atgacggctt gcttcttttc tttcgatacc atttgctctg
    37801 cggctccaga cgttaccacc caacccgccg caaaaccgac aatcgcgatg gcgaccgcgc
    37861 cgactacagc accccacacc gcgggcttca cccattctgg aatctgaaaa ttcatgattt
    37921 cctccctgtg ctggatttac ttcactgatt gattctgacg atttacatga atttgatgat
    37981 acagcgctca tgcctcgatg caacccgcgc tcgtggtatt ttttccgttc gcctcactgt
    38041 ctgcctcttc gccgtgaaat caaacgtagt catcgcggct caaaacagcg gcaatgagat
    38101 tgggatgaac ggttaaccgt tcagcattat gggcggttaa cccttcgcaa attatcagtt
    38161 catgcgaatt gcctttgttt cgcaaattcg gcactcgcaa gctcagagcg gtgatttagc
    38221 cgacagcagc cttaaggcgg ctcgttgtgc gatgagcagg gtattcgcga gaacagatcg
    38281 cccagctccg agaagccgac ggatccaacc aatcggatcg aggcagatgc ggggtgcaaa
    38341 cagcagcccc acatccgaag catcctgacg ggcttgcaat cggcgcgcgc atccgctagt
    38401 gacattgcgt gctcacgccc ggttcgccgg gccaggtaac cggaacccgc agactgccga
    38461 tgcccacgat gaccagtgga cctgccagag agacggacgt ggccgttgcc gcgccggcag
    38521 gtggctttcg tctgcacgcc ctccttcgca acctgctcct taccagcccc tgagcctcga
    38581 cggtctcggg acgtcccggg acgggcgctc aggggggtgt agtgaagcaa atccgatcct
    38641 gatatcactg tcggcctcct tgtgccggct tcgcgaagga aaatctcagc atgtctcaaa
    38701 cgaccaatcc cggcgaccag gtattgatct tcgacacgac gctgcgcgac ggcgagcaat
    38761 gccccggcgc gaccatgaca ttcgaagaga agctcgaggt cgccgacctg ctcgaccaga
    38821 tgggcgtcga catcatcgag gcgggctttc ccatcgcctc ggacggcgac ttcgaggcgg
    38881 tggcggccat tgccaaacgc gtgaagcgcg ccgtcgtggc cgggctgtcc cgcgccggat
    38941 tcaaggacat cgaccgctgc ggcgaggccg tcaaatacgc ccgccggccg cgcatccaca
    39001 ccttcatttc cacctccccg atccacatga agtacaagct tcagaaggag ccggaggagg
    39061 tgctggagat ggtccgcgca tccgtcgagc gtgcccgcaa tcatgtcgac gatgtcgaat
    39121 ggtcagccga ggacggcacg cgcacggaga tcgatttcct gtgccgatgc gtcgagatcg
    39181 ccatcaaggc cggtgccacg acgatcaata tccccgacac ggtcggctac acgactccgg
    39241 aggaatactt ccagctcttc acgacggttc gcgagcgcgt gccgaattcc gacaaggccg
    39301 tcttctcggt ccactgccac aacgatctcg gcatggcggt cgccaattcg ttggccggcg
    39361 tgaaggcggg tgcgcggcag gtcgaatgca ccatcaacgg catcggcgag cgcgccggca
    39421 atgccgcgct cgaagagatc gtgatggcga tcaagacgcg caacgacgtc ctgccgtaca
    39481 cgacaggtat cgagacgacg atgctgaccc gtgcctcgaa gctcgtcgcg gcggcgacat
    39541 cctttcccgt gcagtacaac aaagccatcg tcgggcggaa tgccttcgcg cacgagagcg
    39601 gcatccacca ggacgggatg ctgaagcaca cccagacata cgagatcatg acgccggaat
    39661 cggtcggcgt gagcaagacc tcgctggtga tgggcaagca ttcggggcgg aatgccttcc
    39721 gcaccaagct caaggagctg ggctacgatc tcggcgagaa tgccctggag gacgcgttca
    39781 accgcttcaa ggctctggcc gaccgcaaga aggtcatcta cgacgaggac atcgaagctt
    39841 tggtcgatga agggatcgca accgccaccg accgcatcaa ggtggtggct ctgacggtga
    39901 tcgccggcac ctccggcccg cagtcggcga cgctgacgct cgacatcgat ggcaagcatc
    39961 agaccgtcca ggcgaccggc aacggcccgg tcgatgccac cttcaacgcc atccatgccc
    40021 tcgtgccgca cacggccgtg ctggagctct accaggtcca tgccgtcacc gaggggacgg
    40081 atgctcaggc cgaggtgtcg gtgcgcctgc acgaaaacgg ctacacgtcc agcgccaagg
    40141 ctgctgaccc ggacaccctc gtcgcatctg caaaggcgta cgtggccgcg ctcaacaagc
    40201 tcctgatcaa gcgtcagcgc ggcgccccgg cccagcactt cgccgagccg gcggccaatc
    40261 tgcgcggggt ctgaacgcgc tcgtgccccg gccgtgcctt ccggaggccg gggtgctgcc
    40321 tgccttttgc tctagctggc gttggcggca aagccgaatt cgcatatgct tgccgccata
    40381 tgctgggccg cgcatttcgt ggctcgagac cagggatttg caacagtgtc accgtcggcg
    40441 gccgatcggg tggggcgtat tgtacggccc gtcggcacct gtgtacgacg ctgctaggtg
    40501 tcctcggagt tgaagggggc acctgggatc gtaaagtcgg tcccgtgatt cgtcacaccc
    40561 gccgacgccg aggcatcagc cccatgctgc cgaaattttc aggagttttt tccgaagaca
    40621 ttgcgatcga tctcggcacg gcgaacaccc tcgtctatgt gctcggccgc gggatcatcc
    40681 tcgacgagcc atcgatggtg gcgatccgct cgagcgccgg agagcgcgag atggttgccg
    40741 tcggggcgga ggcccgccgg atcatcggcc tgacgcccga accgctcgag gcgctctgtc
    40801 cgctccgcga cggggtgatc gccgatttcc tcgccaccga gttcatgttg cgccagttca
    40861 tccggcgcgc ccgctcgatc ctgaactttc gccgccctcg catcctgatt tccgtcccgg
    40921 ccggcgccac cccggtcgaa cgccgcgcgg tcgtcgagac cgcccgctcc gccggcgccc
    40981 gcaccgttgc cctcatcgcc gaaccggtcg ccgccctcct gggcgcgggc gttccggtct
    41041 acgatgcgac cggctcgatg gtcgtcgaca tcggcggcgg cacgaccgac atcgccatcg
    41101 tctcctacgg caaggtgatc accgcccgct cgctgcgctg cgccggcaac gcgatggacg
    41161 aagcgatcat tcgctatgtc cggcgccgtc atcatctcgt catcggcgag gcgagcgccg
    41221 aacggatcaa gatcgaggcc ggctcggcca gcgccgtcgc caatggacgc gacgcggata
    41281 ttcacatccg cggccgcgac ctgcgccgcg gccgtctgaa gagcgtcgtg ctcgggccac
    41341 cgcacatcgc cgaggcgctg gatcagccca tcagcgagat caccgagttc gcccagcgcg
    41401 cgctcgagga ggtgcctgcc gaaatcgccg cttcgattta cgagcgcggc atcgtgctga
    41461 ccggaggggg agccctcctc gaccggctcg atcacgagct cacccgccgc cttggcattc
    41521 ccttcgctct ggccaaggac ccgatgcgct gcgtcatcat gggcacggcc atcgtgctgg
    41581 agcacctcgc ggactccggc catctgctgc tgtcgccgga cttctgattt caattcgcgc
    41641 aagcatcatg gtgggctgtc gcgaccaatg ctgccagggt ctggacagcc tgcggcgacg
    41701 atgttagatc aacgtcaccg gggcccagca gggccccttt gccatcgtgg cgcgggtgta
    41761 tagctcagtt ggtagagcag ctgactctta atcagcgggt ccaaggttcg aatccttgta
    41821 cacccaccaa aatttccgcg atgaaacttg agagttagtc cggggcttgc cgcgcctgac
    41881 ccaagccggc gccgccgctc atcagccagg cggcgcagtc gggggtgagt tcgcgggccg
    41941 cgacgtcgtc cgccttggcg atctcggccg cgtccaggag gtcgcgccag cggccgttga
    42001 cggccttgtt gatgaaggtg gtc
//
LOCUS       DASVDU010000256         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1219639, whole genome shotgun sequence.
ACCESSION   DASVDU010000256 DASVDU010000000
VERSION     DASVDU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219639"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..494)
                     /locus_tag="VF226_16865"
     CDS             complement(6..494)
                     /locus_tag="VF226_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7075711.1"
                     /translation="MRVRRGKPADAQRLAEVFRESWQLAYRGIIPHLHLESMIRRRGP
                     EWWASAVRSGDSVYVLEVGGEIAGYATWGTARSRGPYQGEIYELYLAPIYQGLGFGEL
                     LFETCRHAMDQRRIKGLLCWALAENTPAINFYWRRGGRPIAKAFDRIGGAKLEKIAFG
                     WT"
     gene            complement(672..1127)
                     /locus_tag="VF226_16870"
     CDS             complement(672..1127)
                     /locus_tag="VF226_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX7075712.1"
                     /translation="MNEQFTRQAQEMFSAAKDARIPENVQAFAEESVAKTRDLYQKMN
                     AAAKDNVKAVEEVVMTAQSGARALSEKVLNNAAANADAAFEAAQAIARARTIPEAARL
                     QADFIQQQIAAASLQTKELFELSTKMAQQTFACMNSAAKRCYLNAKTDA"
     gene            complement(1297..2622)
                     /locus_tag="VF226_16875"
     CDS             complement(1297..2622)
                     /locus_tag="VF226_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7075713.1"
                     /translation="MTAPRPASSFPWPDGVQVTREHELAAEFPATGSGHLFVTGRAGT
                     GKSTLLRCLREIIAGEGVVLAPTGLAAVNVGGQTIHSFFGFPPRLIRPDDIRRSRNGG
                     IMRRLKFLIIDEVSMVRSDLMWAIDQALRVNRGRPREPFGGVRLLLFGDLHQLPPVVQ
                     EAEVADHLEASFGGPFFFSVPALQDGDGTAYIELRHVFRQSDKDLVGVLDRIREGEVG
                     PDDLELLNSRVCPIRTLAEGDPFVILTPTNAAANRINLAYLEALPGSVHTYEAGVTGD
                     FSDNAHPTDSTLVLKEGAKVILLRNDPDRRWVNGTIARISRLDERQVWVEIDGKEYEV
                     EPVAWENRRYAYDLSAQKIVETVAGTFKQFPLRLAWALTIHKSQGLTLDRVYIDLGRG
                     TFAHGQAYVAISRCRSLAGLAFARPLRPQDVLFDPAVMGYRDLFPALSS"
     gene            complement(2640..4469)
                     /gene="typA"
                     /locus_tag="VF226_16880"
     CDS             complement(2640..4469)
                     /gene="typA"
                     /locus_tag="VF226_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7075714.1"
                     /translation="MNLRNIAIIAHVDHGKTTLVDQLLKQSGAFRENQRVAERAMDSN
                     DLERERGITILAKCTSLVWRDTRINIVDTPGHADFGGEVERILNMVDGAIVLVDASEG
                     PLPQTKFVVSKALNRGLRPIVAINKIDRPDERHLDVLNEVFDLFASLDATDEQLDFPV
                     LYGSGRDGWMALDPAGPKESMAPLFELVLSHVPAPQVEDGPFRMLATTLEADPYLGRI
                     LTGRITSGSVRPNQTIHALHHDGRLIESARVSKVLAFRGLERQPIEVGEAGDIVAIAG
                     VTEATVADTLCDPSVEVPLPAQPIDPPTLSMTFRINDGPLAGQEGDKVQSRVIRDRLF
                     KEKERNIAIRVSESDEKDAFEVAGRGELQLAILIETMRREGFELTVSRPRVVYKNDPE
                     TGQRLEPIEEVIIDVDEQFSGVVVQKISERRGELIEMRPSGGGRQRLVFRVPTRGLLG
                     YQSELLSDTRGTAVMNRLFHGYAPYKGEIQGRRTGVLISNGDGEAVAYALFNLEDRGP
                     LMIGPQTRVYQGMIVGEHSRENDLEVNVLKGKKLTNIRAAGKDDNVILTPPMKMSLER
                     ALSYIADDELVEITPKSIRLRKRWLDPNMRKKMERLRGAEAVA"
     gene            complement(4572..5261)
                     /locus_tag="VF226_16885"
     CDS             complement(4572..5261)
                     /locus_tag="VF226_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075715.1"
                     /translation="MPLQNRVTPAGDIVAIAARGTMMGNRGGCLHRPDKTLGRRRWVT
                     KQWICCELQFRGRHRAVMAPNQYTELFFLDEATALAAGHRPCFECRRADALAFARLWA
                     QSEGRTGRASAAEMDSVLHAERLNAGGAKRVFQSRLADLPSGCIVRLGDRPYLVTGGE
                     LRPWTPEGYLAPQRAEPETVADVLTPKTVVELLRLGLQPRLHRSALEPERTDPAPRIL
                     AVDARPSRPSR"
     gene            complement(5387..>5768)
                     /locus_tag="VF226_16890"
     CDS             complement(5387..>5768)
                     /locus_tag="VF226_16890"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599695.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7075716.1"
                     /translation="GGTILLAAHRAIESITLDRGAAHLKDELMPRYAELIYNGFWFSP
                     EREMLQALIDKSQEHVEGEVRLKLYKGNVIVIGRTSPKSLYSPTLVTFEDDRGAYDQK
                     DAEGFIRLNALRLRTLAMRRKSTE"
BASE COUNT          966 a         1861 c         1868 g         1073 t
ORIGIN      
        1 ctggatcagg tccagccgaa cgcgatcttc tcgagcttgg ccccgccgat gcgatcgaat
       61 gccttggcga tcgggcgtcc gccacggcgc cagtagaagt tgatcgccgg cgtgttctca
      121 gccagagccc agcagaggag acctttgatc cgccgctgat ccatggcatg acggcaggtc
      181 tcgaacagca gctctccaaa gccgaggccc tggtagatcg gcgcgaggta aagctcgtag
      241 atctcgccct gatagggtcc gcggctgcgc gccgtacccc atgtggcgta cccggcaatc
      301 tccccgccaa cctcgagcac gtaaacgctg tcgccggagc gcacggcact cgcccaccac
      361 tcggggccgc gacgacggat catgctttcc agatgaaggt gtggaataat tccgcgatag
      421 gcaagctgcc aggactcgcg aaaaacctcg gccagccgct gagcgtcggc cggcttgccg
      481 cgtctgaccc gcacttgtga gctggacccc aacatgggtc cagtattaag cgcggagtcc
      541 aagaagatgc aacgctcttt tgtcggattc gttccgcccg cgttcacaag tcacatcctt
      601 tggttgtgaa gtgaagcaaa ggcccgacat tgctcgggcc ttgccataag ttcggggggc
      661 gtccgagcca atcaggcgtc ggtcttggcg ttcaaatagc aacgcttggc agccgagttc
      721 atgcaggcga acgtctgctg cgccatcttg gtcgaaagct cgaacagctc tttcgtctgg
      781 aggctggcgg ccgcaatctg ctgctggatg aagtcagcct gcaggcgggc ggcctcaggg
      841 atggtgcgcg cacgcgcaat ggcctgggcg gcctcgaagg cggcatccgc attggcggcg
      901 gcgttgttca gcaccttctc gcttagcgcg cgggcgccgg actgagcggt catcaccacc
      961 tcctcgacgg ccttcacgtt gtccttcgct gcggcattca tcttctggta gaggtcgcgg
     1021 gttttcgcca cgctctcctc ggcaaaggcc tgcacattct caggaatgcg cgcatctttg
     1081 gcggcgctga acatctcctg cgcctgacgg gtgaactgct cgttcatggg actctctcct
     1141 gaaattccga ttgcacggtg cgctctggga cctgggggtt cgatttccgc cacatccgat
     1201 gatcagtact tagacagttc tattgtgcaa tgcaataaaa tttgtgcgac gcagtaagtg
     1261 ggcaaggtgt cgcatgctcg ccgcaggggg tcggagctaa ctgctcagcg cggggaacag
     1321 gtcgcgatag cccatgacgg cggggtcgaa cagcacgtcc tgcggccgca gggggcgtgc
     1381 aaacgccaaa ccggcgagcg accgacaccg cgagatcgcc acgtaggcct gcccgtgggc
     1441 gaacgtgccg cgcccgagat cgatgtacac ccggtcgagt gtgagtccct ggctcttgtg
     1501 aatggtgagg gcccaggcga gccgcagcgg gaactgcttg aaggtgcctg cgaccgtctc
     1561 cacgattttt tgggcggaca ggtcgtaggc atagcgccga ttctcccacg cgacgggctc
     1621 gacctcgtac tccttgccgt cgatctcgac ccagacctgg cgctcgtcga gtctgctgat
     1681 gcgcgcaatg gtgccattga cccagcgccg gtcggggtcg ttacgcagca ggatcacctt
     1741 ggcgccctcc ttcagcacaa gcgtgctgtc agtcggatgg gcgttgtcgc tgaagtcgcc
     1801 ggtgacaccc gcctcatacg tgtgcaccga gccgggtagc gcttcgaggt aggcgaggtt
     1861 gatgcgattg gcggcggcgt tggtcggcgt caggatgacg aatggatcgc cttcggcgag
     1921 cgtgcggatt gggcagacac ggctgttgag caactccaga tcatccggtc cgacctctcc
     1981 ttcgcgaatg cgatcaagga ccccgaccag atctttatcg ctctggcgga acacgtgtcg
     2041 aagctcgatg taggctgtgc cgtcaccgtc ctgcagggcc gggacactga agaagaacgg
     2101 gccgccaaag ctcgcctcca ggtgatcggc aacttccgct tcctgcacga ccggcggaag
     2161 ctgatgcaag tccccgaaca gcagcagccg taccccgccg aacggctccc gtgggcgacc
     2221 gcggttgacg cgcagcgcct ggtcgatcgc ccacatcagg tccgaccgca ccatcgacac
     2281 ttcatcgatg atgaggaatt tgagccggcg catgatgccg ccgttgcggc tgcgccggat
     2341 gtcatcgggc cggatcaggc gcgggggaaa gccgaaaaag gaatggatcg tctgtccgcc
     2401 gacgttgacg gcggcgagcc cggtcggggc cagcacgacg ccctcgccgg caatgatctc
     2461 acgcaggcag cgcagcagcg tcgacttgcc ggttccggcg cggccggtga cgaacaggtg
     2521 gccacttccc gtagcgggaa attcagccgc cagctcgtgc tcgcgggtga cctgaacccc
     2581 atccggccat gggaaactgc tggctgggcg aggtgcggtc accgtgtccc ttctcctgct
     2641 caggcgacgg cctcagctcc tctcagccgc tccatcttct tgcgcatgtt ggggtcgagc
     2701 cagcgtttgc gcaagcggat cgatttgggc gtgatctcga cgagctcgtc atccgcgatg
     2761 taggagagcg cgcgttccag cgacatcttc atcggcgggg tgagaatgac gttgtcgtcc
     2821 ttgcccgcgg cccggatgtt ggtcagtttc ttgcccttga gcacgttcac ttcgaggtcg
     2881 ttttcgcgcg agtgctcgcc gacgatcatg ccctggtaga cgcgcgtctg cggcccgatc
     2941 atgagcggcc cgcggtcctc aaggttgaac aaggcgtatg ccaccgcctc gccgtcgccg
     3001 ttcgagatga ggacgccggt gcgccggccc tggatttcac ccttgtaagg cgcgtagccg
     3061 tgaaagagcc ggttcatcac ggccgtgccg cgcgtgtccg acaagagctc gctctggtag
     3121 ccgagcagcc cgcgcgttgg cacgcggaac accagccgct gccgtccgcc gcccgacggg
     3181 cgcatctcga tcagctcgcc gcggcgctct gagatcttct ggaccacgac gccgctgaac
     3241 tgctcgtcga cgtcgataat gacctcctcg atgggctcga ggcgttgtcc cgtctcagga
     3301 tcgttcttgt agacgacccg tggtcgggac accgtcagct cgaagccctc ccggcgcatg
     3361 gtttcgatca ggatcgcgag ctgcagctca ccgcggccag ccacctcgaa cgcgtccttc
     3421 tcatcggact cgctgacgcg aatggcgata ttgcgctctt tttccttgaa taagcggtcg
     3481 cggatgacgc ggctctgaac cttgtcgcct tcttggccgg cgagcggccc gtcgttaatg
     3541 cggaaggtca tcgacagcgt cggtgggtcg atgggctgcg ccggcagcgg cacctcgacg
     3601 gaagggtcgc acagcgtgtc ggcgaccgtc gcctcggtca cacccgcgat ggcgacgata
     3661 tcgcccgcct cgccgacctc gatcggctgg cgctcgagcc cgcggaaggc cagcaccttg
     3721 ctgacgcggg ccgactcgat gaggcggccg tcgtgatgca gcgcgtggat cgtctggttg
     3781 gggcgcacgc tccccgacgt gatgcggccc gtcaggatgc ggcccagata cgggtccgct
     3841 tccaatgtgg tcgccagcat ccgaaagggg ccgtcctcga cctgtggcgc cggtacgtgc
     3901 gacagcacca gttcgaacag cggcgccatg ctctcctttg ggccggccgg atcgagggcc
     3961 atccagccgt cacggccgga gccgtagagc accgggaaat cgagctgctc gtcggtcgca
     4021 tccaggctgg cgaagaggtc gaaaacttcg ttcagaacgt cgaggtgccg ctcgtcgggg
     4081 cggtcgatct tgttgatggc cacgatcggc cggagtccgc ggttcaacgc ctttgaaacc
     4141 acgaacttcg tctggggcaa cggcccctcc gaggcgtcga ccagcacgat tgcgccgtcg
     4201 accatgttga gaatgcgttc cacctcgccg ccgaagtcgg catggcccgg cgtgtcgacg
     4261 atgttgatgc gcgtgtcgcg ccacaccagc gaggtgcatt tcgcgaggat ggtgatgccg
     4321 cgctcgcgtt cgaggtcgtt gctgtccatc gcccgctcgg ccaccctctg gttctcccgg
     4381 aaggcgccag actgcttcaa gagctggtcg accagggtcg tcttgccgtg gtcgacgtgg
     4441 gcgatgatgg cgatgttacg aaggttcatg aagctcacgt gttgcggcgc ggaaaatcag
     4501 gcgccgaggt atcacagttg gtccgatctc gctaggagcg cacccgcagc cgtggcggat
     4561 tggccgcctc gtcatcgcga cggccgcgaa ggccgcgcgt ccacagccag gattcggggc
     4621 gctggatccg tcctttccgg ctctagggcg ctccggtgaa gacgcggctg caacccgagg
     4681 cgcaacagct ccaccaccgt cttcggggtc agcacgtccg cgactgtttc gggctcggca
     4741 cgttgaggcg ccaggtaacc ctcgggtgtc cagggccgaa gctccccacc tgtcaccaga
     4801 taagggcgat ctcctaaccg cacaatgcat cccgatggta ggtcggccaa ccggctctga
     4861 aagacgcgct tcgccccgcc tgcattcagc cgttccgcgt gcaggacgga atccatctcc
     4921 gccgcgctcg cccgcccagt ccggccctcg gattgtgccc acagccgggc gaacgcgagc
     4981 gcatcggccc ggcggcactc gaagcagggc cggtggccag ccgccagcgc cgtcgcctca
     5041 tccaggaaga acagttcggt gtattggttg ggcgccatga ccgcgcggtg acgaccgcga
     5101 aactgcagct cgcagcagat ccattgcttc gtaacccagc ggcggcgccc cagggtcttg
     5161 tccggccgat gcaggcatcc tccccggttg cccatcattg tcccgcgcgc ggcgatcgcg
     5221 acgatgtccc ctgccggcgt gacgcggttc tggaggggca tggcgctgca gctccaaaca
     5281 gacagtagtc atcccggtgc ttgtcaccgg gatccactca gccgcttgcg ccggagtctg
     5341 aggaaagctg gaccccgggc acaaggcccg ggatgaccgc tgttgcttat tccgtcgact
     5401 ttcgacgcat cgccagcgtc cgcagcctca acgcattgag gcggataaag ccttcggcat
     5461 ccttctggtc gtaggcgccg cggtcgtcct cgaaggtgac gagcgtcggg gagtagagcg
     5521 acttcgggct cgtacggccg atgacaatga cgttgccctt gtagagcttc agccgcacct
     5581 cgccttcgac gtgctcctgg ctcttgtcga tcagcgcctg cagcatctcg cgctcaggcg
     5641 agaaccagaa gccgttgtag atgagctccg cgtagcgcgg catcagttcg tccttgagat
     5701 gggcagcacc ccggtcgagc gttatcgatt cgatggcgcg atgggcggcg agcaggatgg
     5761 tgccgccg
//
LOCUS       DASVDU010000257        11001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1680917, whole genome shotgun sequence.
ACCESSION   DASVDU010000257 DASVDU010000000
VERSION     DASVDU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680917"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1452)
                     /locus_tag="VF226_16895"
     CDS             complement(64..1452)
                     /locus_tag="VF226_16895"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7075717.1"
                     /translation="MTQFTELGLAAPILKALAGEGYTTPTPIQAKAIPAVLAGSDLLG
                     IAQTGTGKTAAFALPTLHRLATDRRRAARKGCRVLVLTPTRELASQIADSFKTYGRHL
                     RLSVAVVFGGVGHRPQIQALASGVDILIATPGRLLDHMGARAVNLQDVEILVLDEADR
                     MLDMGFVRPIRQIVSHLRTDRQTLLFSATMPQEIAGLAGSFLRTPVKVAVTPVASTAE
                     RVSQRVIHVESQKKRALLAELLSDPSMSRTLVFTRTKRGADRVAKHLDEAGIRVAAIH
                     GNKSQKQRELALDAFRRARIRVLVATDIAARGLDVDGVSHVVNYELPDVPEDYVHRIG
                     RTARAGASGSAISLCDAGERALLRSIERLTHQAIESEDRRSDSSLTTEKSPERSDRER
                     LRPERSDVSSAGRHAARRAGTGGAPGRGTRCNGSAPEPTPIPPSRSSSIVRRQRVRGK
                     PARLRTRGLTFQ"
     gene            complement(1823..2917)
                     /locus_tag="VF226_16900"
     CDS             complement(1823..2917)
                     /locus_tag="VF226_16900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7075718.1"
                     /translation="MSALPKEEGVPFDAARSEAFAERFLKALNESAMVLMTSLGHRTG
                     LFDALDGAAPLTSEELARKAGLNERYVREWLAVMVMSHVVEYEPRSQRYRLPPEYAAF
                     LTRASVPNNLAATAQYISGIGSVETQLAECMRKGGGLPYTAYHRFHEVMAEDSGQTVV
                     AALFDAILPLVPGLTERLEAGISVADVGCGAGRAMIALAEHFPKSRFTGFDLCEEPIE
                     TANRQVRTKALTNISFEQRDLSRRPLEGPFDLITAFDAVHDQRDPAGLLRSIHDALAP
                     TGVFLMQDIAGSSHLEKNFDHPVAALLYAVSTAHCTCVSLGQGGPGLGTMWGEELAEK
                     MLREAGFGEITRERLPHDFMNVYFIARHRQ"
     gene            3021..4061
                     /locus_tag="VF226_16905"
     CDS             3021..4061
                     /locus_tag="VF226_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7075719.1"
                     /translation="MPDGDGRLSVALLAVPEATPSTIYGLYDLFASVGRDWDLVVKGR
                     PGRSLMRSEIVSAGGEPVSGANGSTIPAARAIEDCPRPDIVCVPEVLCPPDCLDTAVY
                     ESACAWLRRTYEAGSVIASVCSGALLLAQSGLLDGREATTHWGFCGTLRSRYPAVTVH
                     ESRALVATGEGQRIVTGGGGSSWHDLALYLVARFAGVEEAVRIAKLYLIEWHAHGQLP
                     FASLSLTRQVRDPLIAACQEWIADHYAEPRPVRAMIVRSGLPERSFKRRFTQATGMSP
                     IEYVHTLRLEEAKQILETTDTPVEAIAEEVGYEDGSFFRRLFRRKVGLAPAEYRRRFA
                     PFRRALNGNARQ"
     gene            complement(4137..5156)
                     /locus_tag="VF226_16910"
     CDS             complement(4137..5156)
                     /locus_tag="VF226_16910"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX7075720.1"
                     /translation="MKPKTFVITGLAAVATGIAAVAVYTTHNQWDPGRIGGEKLVPAL
                     ASGTADVGALVVTHGGKTLTIQSNADGQWTLKESDGYPADPDKVRALLVKLAQAELVE
                     AKTRMPDRHALLELEDPAGKDAKSRSLKMLDGRGRVIADLVIGKRRWEAFGSGKSGTY
                     VRKRDDAQTWLANAEINLQADTRYWIEPTIFNTQSDKIQRLTLHIPGEEMLKIERGSG
                     DQDKPAFVGFPENAKLKDSSAADGILRAAARIDVDDVRKLDGPPSGDVSTLSFTTKDG
                     LEVTFWVRKDGDDYWLSLAATGDGDAKKTAENIRKQTDGWEYKISSAKAGSLLKRRAD
                     LIESS"
     gene            complement(5187..7169)
                     /locus_tag="VF226_16915"
     CDS             complement(5187..7169)
                     /locus_tag="VF226_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX7075721.1"
                     /translation="MNSKLTAPFEKAAEWASRLPRHTMAWTGLVLAGVVFLSINLISS
                     TAFRSVKADFTKEGLYTISEGTRKALSSIDEPIDIRVYYSNKLGEAAPSFAKNFERVR
                     TLLEQYRSISGGKLQVSYIDPEPFSDAEDRAVAAGLRGIRLNPEGDLGYFGMVGANST
                     DNEETIGFFAPERERFIEYDVTKLIYTLAHPKKPTIGLVSTLPLDGGLDPMMGMRGPV
                     MQPQMVMQQIRDLFEVKTLGKDLKEVPADVDVLMVVQPDGLSPEAVYAIDQFALKGGR
                     VLAFVDPVPETLRPATPMAMSTSPNFEEFNKLLKAWGVEFDGKQVAGDIQHARRVQFG
                     GGPNNVVTEYVAWLGFDSSNLDKGDVLSAGIERLNLASSGYFTPVKGATSKLTPVLQT
                     SAEAEIIPAEKISPMPDAVALLREYKPGGKPLVIAARISGEVKSAYPDGRPKAEKSEE
                     KKDEKNASAAPSNTEEAKDADGKAAHRAEGKINVLVVADTDLLSDQFWVDVRDFLGQQ
                     IAMPHAHNSAFVIGALENLSGSDALLSLRGRGVVDRPFELVADIRREAERRYREKEQA
                     LTAKLKELQDQLAKLEKVGDGEAVILTDKDREAIERFRGEMLTVRRDLREVKRALRED
                     IDRLDAWLKIANIGAVPLLLGAGAIGWAAWRRRRRS"
     gene            complement(7186..7932)
                     /locus_tag="VF226_16920"
     CDS             complement(7186..7932)
                     /locus_tag="VF226_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX7075722.1"
                     /translation="MLDSLKRTWVIAKREFGAYFATPLALVFLIIFVALTGAFAFYVG
                     NFFDRNQADLTSFFAYHPWLFLLLVPAIAMRLWAEERKTGTIELLMTLPLSPAEAIVG
                     KFVAAWAVIGVALILTFPMWLTVNFLGSPDNGVILASYVGSFLTAGAFLAIGSCISAL
                     TKNQVIAFIVAAAVCFLLLMSGMDLVLNAFRGWAPQFVIAAIQSLSFLSYFEDFSKGI
                     VSLPALIFYASLIAFALFANRIVIDQKKAA"
     gene            complement(7925..8887)
                     /locus_tag="VF226_16925"
     CDS             complement(7925..8887)
                     /locus_tag="VF226_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075723.1"
                     /translation="MPALIEATELKKSFGSIKAVNGISLTVTSGEVLGFLGPNGAGKS
                     TTMKMITGFLEPDSGTARIAGYDVQEQPLAAKARLGYLPEGAPAYAEMSPASFLRFVA
                     AVRGFRGAEAQRRVDIAVEKTGLQSVLNQTIETLSKGFKRRVGIAQAILHDPDVLIMD
                     EPTDGLDPNQKHHVRELIREMARDKAIIVSTHILEEVEAVCSRAVVIDKGRIVADGTA
                     EDLRQRAPYHGAIAIRVSSADADLIKTALQRFTSIAAVETVGRVNGHLHLRALPKSGM
                     SIAAEVTQAIRESAIVVDEIYVERGKLDDVFRQITTSDQEAGHA"
     gene            complement(9047..10675)
                     /gene="groL"
                     /locus_tag="VF226_16930"
     CDS             complement(9047..10675)
                     /gene="groL"
                     /locus_tag="VF226_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602475.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7075724.1"
                     /translation="MSAKEVRFSHDAREGMLRGVDILANAVKVTLGPKGRNVVIEKSF
                     GAPRTTKDGVTVAKEIELDDKFENMGAQMLREVASKTNDLAGDGTTTATVLAQAIVRE
                     GARSVAAGANPMDLKRGVDLAVNAIVADLKKNSKAVTNNDEIAQVGTISANGDSEIGK
                     MIAEAMEKVGKEGVITVEEAKSLETELDVVEGMQFDRGYISPYFITNAEKMTVEFDDP
                     YVLIHEKKLSSLQPLLPVLEAVVQTGKPLLVIAEEVENEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTSGQVVSEDLGIKLENVTLDMLGRAKRVTIDKDNTTIVG
                     GAGKKENIDARINQIKQQIETTTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKER
                     KDRVDDALNATRAAVEEGIVPGGGVALLRALKALDSLNPANEDQKTGINIIRRAIQAP
                     ARQIAQNAGEDGSVIVGKVLENSSYAWGYDAQTGEYGDLVAKGIIDPTKVVRTALQDA
                     ASVAGLLITTEAMIAEKPKKADAAPAMPGGGMDF"
     gene            complement(10801..>11001)
                     /locus_tag="VF226_16935"
     CDS             complement(10801..>11001)
                     /locus_tag="VF226_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7075725.1"
                     /translation="EVLAVGPGTRDENGKITPLDVKPGDTVLFGKWSGTEVKIDGEEL
                     LIMKESDIMGVLEGRAKTKAAA"
BASE COUNT         1891 a         3511 c         3504 g         2095 t
ORIGIN      
        1 gaaatcatgt ttgagacatc agaaattgcc ctcggaacgt gagttcaggg ttatcagcct
       61 tcactactga aacgtcaagc ccctcgtgcg caggcgtgcc ggtttaccgc ggacgcgttg
      121 ccgtctgaca atgctcgatg atcgcgatgg aggaatgggg gtgggctcag gcgctgaccc
      181 gttgcaccgg gtccctcgcc ccggagctcc gccggtgcct gcgcggcgag cggcgtgacg
      241 tccagcgctg ctgacgtcgg agcgctccgg acggagcctt tcccgatcgc tccgttcagg
      301 actcttttcc gttgtaagac tggaatcgct gcgccggtcc tcggactcga tggcctgatg
      361 ggtcaggcgc tcgatgctac gcaggagagc gcgctcgccg gcgtcgcaga gcgagatggc
      421 gctgcccgag gcgcctgcgc gcgctgtgcg cccgatgcgg tgaacgtagt cctcgggaac
      481 atccggcagt tcatagttga cgacgtgcga cacgccgtcg acatcgaggc cgcgcgcggc
      541 gatatcggtt gcgacgagca cgcggatcct ggccctccgg aaggcatcga gcgccagctc
      601 gcgttgcttc tggctcttgt tgccgtggat cgcagcaacg cggatgccag cctcgtcgag
      661 atgcttcgcg acgcgatccg cgccccgttt tgtgcgggtg aaaacaaggg tgcgcgacat
      721 gctggggtcg gaaaggagct cggcgagcag cgcgcgcttc ttttggcttt cgacgtggat
      781 aacacgctgg ctgacgcgct cggccgtcga cgcgacgggc gtgactgcga cctttacggg
      841 cgtgcggagg aacgagccgg caaggccggc gatctcctgc ggcatcgtcg ccgagaaaag
      901 gagtgtttgc cggtctgtcc gcagatgcga gacgatctgc cggatgggcc tgacgaagcc
      961 catgtccagc atgcggtcgg cctcatcgag caccaggatt tcgacatcct gcaagttgac
     1021 cgcccgcgct cccatgtggt cgagcagccg ccccggcgtg gcgataagaa tgtcgacgcc
     1081 gctggcgagc gcctggattt ggggccggtg gccgacgccg ccgaacacga ccgccacgga
     1141 caggcgcaga tgacgcccgt aggtcttgaa gctgtcggcg atctgcgagg caagctcgcg
     1201 cgtcggcgta aggacgagga cacggcagcc cttgcgagcg gctcgccgac gatcggttgc
     1261 aagccggtgc agggtcggga gggcgaaagc cgccgtcttg ccagtgccgg tctgtgcgat
     1321 gccgagcagg tcggagcccg cgagtacggc cgggatcgcc ttggcctgga tgggcgtggg
     1381 cgtggtatag ccttcgccgg cgagcgcctt caggatgggc gcggcgaggc cgagttcagt
     1441 aaattgagtc acagatgtgc tttctcatgc ggccggactg cggccgcgac gcaatcgtcg
     1501 gcgggtctcg caaggcacgg ccttgttcgc aagacgcccc gcgtggcctg ggctgtcgga
     1561 tgggaagtcg ttcagggcgc tcaacagggg aacgaaaggc ggcaaaggcc ggagcctgag
     1621 atttcgaaac gtcccttcac gcggctggag cgccggaaac cgctttgacc agcggtgcgc
     1681 atgggatggc atgctgcagc gcaaaagtca actgttcagg gatcagaagc ttggagcggc
     1741 cgcagggaac atgagaccgg cagcaccgcg cggttgaggg cgcagcgctg ccggcgggga
     1801 cgcatagctc accccgcagc ggtcactggc gatgccgcgc gatgaaatag acgttcatga
     1861 aatcgtgcgg caggcgctcg cgcgtgatct cgccgaagcc ggcctcgcgc agcatttttt
     1921 cagctagctc ctcgccccac atggtgccga ggccggggcc gccctggccg agcgacacgc
     1981 aggtgcaatg cgccgtcgag acggcgtaga gcagggcggc gacggggtgg tcgaagttct
     2041 tctcgaggtg gctcgaccct gcgatgtcct gcatgaggaa cacgccagtt ggggccagtg
     2101 cgtcgtggat cgaccgcagc agcccggcag ggtcgcgctg gtcgtgcacg gcgtcgaaag
     2161 cggtgatgag gtcaaaggga ccttccagcg gccgccggct gaggtcgcgc tgctcgaagg
     2221 agatgttggt tagggctttc gtgcggacct gtcggttggc cgtctcgatc ggctcctcgc
     2281 agagatcgaa gcccgtgaag cggctcttcg ggaaatgctc cgccagagcg atcatagcgc
     2341 gacctgcacc gcaacccaca tcggccacgc tgatcccggc ctcgagcctc tccgtcaggc
     2401 ccggcaccag cggcaggatg gcgtcgaaca aggccgcgac gaccgtctgg ccgctgtcct
     2461 cggccatcac ctcgtggaag cggtgatagg ccgtgtacgg caggccgccg cctttgcgca
     2521 tgcactcggc cagttgcgtc tccacggagc cgattccgga gatgtactgt gccgtagcgg
     2581 caaggttgtt cggcaccgag gcacgcgtga ggaacgcagc atactccggc ggcaggcggt
     2641 agcgttggga gcgaggctcg tactcgacaa catgcgacat caccatgacg gcgagccact
     2701 cgcgcacgta gcgctcgttg agacccgcct tgcgcgccag ctcctcggag gtgagcggtg
     2761 ccgcgccgtc cagggcatcg aacaggcccg tgcggtggcc gagcgatgtc atcagaacca
     2821 tggcggactc gttgagtgcc ttcaggaagc gctcggcgaa ggcttcggag cgcgccgcat
     2881 cgaagggcac gccctcctct ttcggcaaag cggacatcgg ggttgacctt tctgctgtga
     2941 gctgtttttc cgcgcgcaaa ctacttgcgt gctggcccag cggctaaggt cgaataggct
     3001 gcaaacagga cggacatgcc atgcccgatg gagacggacg cctctcggtc gcgctgctcg
     3061 ccgtgcccga agcgacaccc tccacgatct acggccttta cgacctgttc gcgtctgtcg
     3121 ggcgggattg ggacctcgtg gtgaaaggca ggccggggcg ttcgctgatg cggtccgaga
     3181 tcgtcagcgc cggaggcgag cccgtttccg gggcgaacgg cagcaccata cctgcggcgc
     3241 gggcgatcga ggactgcccg cgacccgaca tcgtctgcgt tccggaagtc ctgtgtccac
     3301 cggactgtct cgatacggca gtctacgagt cggcttgcgc gtggctgcgg cgcacctacg
     3361 aggcgggctc ggtcattgca tcggtctgct cgggggcgct actgctcgcc cagtccggtc
     3421 tgctcgacgg ccgcgaggcg acgacccact ggggcttctg cggcaccctg cggagcagat
     3481 acccagccgt gacggtgcac gagtcccgag cgctcgtcgc gaccggcgaa ggccagcgca
     3541 tcgtcacggg tggcggcggc tcgtcctggc acgacctggc gctctacctc gtcgcgcggt
     3601 ttgccggggt cgaggaggcg gtgcgtatcg ccaagctcta cctcatcgag tggcacgccc
     3661 acggacagct gcccttcgcc tcactttcgc tgacgcggca ggttcgcgac ccgctgatcg
     3721 cagcctgcca ggaatggatt gccgaccatt acgccgagcc acgcccggtg agggccatga
     3781 tcgttcgcag cgggctcccc gagcgctcat tcaagcgtcg cttcacccag gccaccggca
     3841 tgtcgccgat cgagtacgtg cacacgctgc ggctcgagga ggccaagcag atcctggaga
     3901 cgacggacac gccggtcgag gcgatcgcgg aggaggtcgg ctatgaggac ggcagcttct
     3961 tccgccgcct gttccgccgc aaggtcggcc tggccccggc cgaataccgc cgccgcttcg
     4021 ccccgttccg gcgggcgctg aacgggaacg cgcggcaatg accgccacca aaagcgaagt
     4081 gccgaccgac gacggccggc gctcacgcag cttgcaggcg gcgagtcgcg cggaggtcaa
     4141 ctgctctcga tcagatcggc acgacgcttc agcagcgagc cggccttggc gctggagatc
     4201 ttgtactccc agccgtcggt ctgcttgcga atgttctccg ccgtcttctt ggcatcgccg
     4261 tcgcccgttg cggcaagcga cagccagtag tcatcgccgt ccttgcgcac ccagaacgtc
     4321 acctccaggc cgtccttggt cgtgaaggag agggtgctga cgtctcccga cggaggaccg
     4381 tccagcttgc ggacgtcgtc cacatcgatc ctcgcggcgg cgcgcaggat gccgtcggcg
     4441 gccgacgaat ccttgagctt ggcattttcc gggaatccga cgaaagccgg cttgtcctgg
     4501 tcacctgagc cgcgctcgat tttcagcatc tcctcgccgg ggatgtggag cgtgagccgc
     4561 tgaattttat cgctttgcgt gttgaagatc gtgggctcga tccagtagcg ggtgtcggcc
     4621 tgcagattga tttcggcatt ggccagccag gtttgagcat catccctctt ccgcacgtat
     4681 gtgccactct tgccactgcc gaaagcctcc caacgcctct tgccgatgac gagatcagcg
     4741 atcacgcgtc ctctgccatc gagcattttg agcgagcgtg acttggcgtc cttgcccgcc
     4801 gggtcctcca gctcgagcag cgcgtggcga tccggcatgc gcgtcttggc ttcgacaagc
     4861 tctgcctggg cgagcttaac gaggagtgca cgcaccttgt ccgggtcggc tggatagccg
     4921 tcgctttcct tcagcgtcca ttggccatca gcattgctct ggatcgtgag cgtctttccg
     4981 ccgtgggtaa cgaccagggc tccgacgtcg gccgtgccgg aagccaacgc cggtaccagc
     5041 ttctcgccgc caatgcgacc gggatcccat tggttatggg tggtatagac ggccactgca
     5101 gcaatgccgg tggcgacagc agccaatcca gttatcacga atgtcttggg cttcatgtcg
     5161 tgatctccag gcaagggcta ttgtcatcag gacctgcgcc ggcggcgcca cgcagcccag
     5221 ccgatggctc cggcaccgag cagcagcggg actgcaccga tgttggcgat tttgagccac
     5281 gcatcgagcc gatcgatatc ctcccggagc gcacgcttca cctcgcgcag atcgcgccga
     5341 acggtcagca tctctccgcg gaaccgctct atcgcctcgc ggtccttgtc ggtgaggatg
     5401 acggcctcgc cgtctccgac cttctcgagc ttcgcgagct gatcctgcag ctccttgagc
     5461 tttgctgtca gggcctgctc cttctcgcga taacggcgct cggcctcgcg gcggatgtcc
     5521 gccaccagct cgaacggccg gtcgaccacg ccgcgaccgc gaagcgagag cagcgcgtcg
     5581 gagccggaca ggttttccag cgcgccgatg acgaacgcag agttgtgcgc atgcggcatg
     5641 gcgatctgct ggccaaggaa gtcccgcacg tccacccaga actgatcact cagcaggtcc
     5701 gtgtcggcga ccacgaggac gttgatcttg ccctcggccc gatgggccgc cttgccatcg
     5761 gcatctttcg cttcctcggt gttcgaaggc gcggcgctgg cattcttttc gtcctttttc
     5821 tcctcggact tttcagcctt gggccggcca tccggatagg cgctcttgac ctctcccgaa
     5881 atgcgcgccg cgatcaccag cggcttgccg ccgggcttgt actcccggag cagggcgacg
     5941 gcgtccggca tcggcgaaat cttctccgcc gggatgatct ccgcctcggc gctggtttgc
     6001 agcaccgggg tcagcttgct cgtagcgcct ttgactggcg tgaagtagcc gctcgaagcc
     6061 aggttcaagc gctcgatccc ggccgagagc acgtcgcctt tgtccaagtt gctgctgtcg
     6121 aagcccagcc aggcaacata ctcggtcacg acgttattcg ggccgccgcc gaactggaca
     6181 cggcgcgcat gctggatatc cccggcgacc tgcttgccgt cgaactccac accccaggcc
     6241 ttgagcagct tgttgaactc ctcaaagttc ggcgatgtcg acatggccat cggggtcgct
     6301 ggccgcagcg tttccggcac cggatcgacg aaggccagca ccctgccgcc tttcagcgcg
     6361 aactggtcga tcgcatacac ggcctcgggc gaaaggccgt ccggctgtac gaccatcagg
     6421 acgtcgacgt cggcgggaac ttccttcaaa tccttgccga gcgtcttgac ctcgaagagg
     6481 tcgcggatct gctgcatcac catctgcggc tgcattaccg gtccgcgcat gcccatcatc
     6541 gggtcgagac caccatcgag cggtaacgtc gagacgagcc cgatcgtcgg cttcttcggg
     6601 tgtgccagcg tatagatcag cttcgtgacg tcgtactcga tgaagcgctc gcgttccggc
     6661 gcgaagaagc cgatcgtctc ctcgttgtca gtggagttgg cgccgaccat tccgaaatag
     6721 ccgaggtcgc cctccggatt gagccggatg ccgcgaagac cggcggccac ggcgcggtcc
     6781 tccgcatcag agaacggctc ggggtcgatg taggagacct gcagcttgcc acccgaaatc
     6841 gaccggtact gctcgagcag ggttcggaca cgctcgaaat tttttgcaaa cgagggagcc
     6901 gcttctccga gcttgttgga ataatagacc cggatgtcga tcggctcgtc gatgctgctc
     6961 agcgctttgc gcgtgccttc cgagatcgtg taaaggcctt ccttcgtgaa atcggccttt
     7021 accgatcgga acgcagtaga gctgatcaga ttgatcgaca ggaaaacgac gcccgcaaga
     7081 acgagacccg tccaggccat cgtgtggcgc ggcaggcgag aggcccactc ggcggctttc
     7141 tcgaatggtg ctgtcaattt cgagttcatg gcgcgctttc tcccttcaag cggccttttt
     7201 ctgatcgatc acgatgcggt tcgcgaacag cgcgaacgcg atcagagacg catagaagat
     7261 cagtgccggc aggctgacga tccccttcga gaagtcctcg aaataggaca ggaagctgag
     7321 gctctggatg gccgcgatca caaactgcgg cgcccatccg cggaatgcgt tgagcaccag
     7381 atccattccg ctcatcagca gcaggaagca aaccgctgcg gcgacgatga aggcgatgac
     7441 ttgattcttg gtcagcgccg agatgcacga gccgatggcc aggaacgcgc cggccgtgag
     7501 gaagctgccg acatagctgg cgagaatgac gccgttgtcc ggcgagccga ggaaattgac
     7561 ggtgagccac atcgggaaag tcaaaatcaa ggccaccccg atgacggccc aggccgccac
     7621 gaatttcccg acgatggcct cagcgggcga gagcggcagc gtcatcaaca gctcgatggt
     7681 gccagtctta cgctcctccg cccacagccg catggcgata gccggcacga gcagaagaaa
     7741 cagccacggg tggtaggcga agaagctcgt caggtccgcc tggtttcgat cgaagaagtt
     7801 gccgacgtaa aaggcgaatg cgccggtcag cgcgacgaag atgataagaa agacaagcgc
     7861 cagtggcgtc gcgaaataag ctccgaactc gcgcttggcg atcacccagg tgcgtttcag
     7921 gctgtcaagc atggccagcc tcctggtcgg acgtggtgat ctggcggaac acgtcgtcga
     7981 gcttgccgcg ctcgacatag atttcgtcga cgacgatcgc ggattcgcgg atggcttgcg
     8041 tcacctcggc ggcaatcgac atcccgctct tcggcagcgc ccgaagatgc agatgaccgt
     8101 tcaccctgcc gaccgtttcg accgcggcga tggacgtaaa tcgctgcagc gccgttttga
     8161 tcaagtcggc atcggcagag gaaacacgga ttgcaatcgc cccgtgataa ggtgcgcgct
     8221 ggcgcaaatc ctcggcggtg ccgtcggcga cgatgcggcc tttgtcaatc actaccgcgc
     8281 ggctgcaaac cgcttcgacc tcctccagaa tgtgcgtcga aacgatgatg gctttgtcac
     8341 gcgccatctc gcggatcaac tcgcgtacgt gatgcttctg gttcggatcc aatccgtccg
     8401 tcggctcatc catgatgagc acatccggat catgcaggat cgcctgggca attccgacgc
     8461 ggcgcttaaa tcctttcgaa agggtttcga tcgtctgatt gaggacggat tggaggccgg
     8521 ttttctcaac cgcaatatcg acacgacgtt gggcctccgc gccgcgaaag ccgcgcaccg
     8581 ccgcaacaaa gcgcaggaaa gatgctggcg acatttccgc gtacgccgga gcgccttccg
     8641 gcaggtaacc aaggcgcgcc ttcgctgcga gtggctgctc ctgcacatcg tagccggcaa
     8701 ttcttgccgt gccggagtcc ggctcgagaa agccggtgat catcttcatc gtcgtggatt
     8761 tgccggcgcc gttcggcccc agaaacccaa ggacctcgcc tgatgtcacg gtcagcgaaa
     8821 tgccgttgac cgctttgatg ctgccgaagc tcttcttcag ttcagtggct tcgatcaatg
     8881 caggcatgcg ccctccctcg agctagagtt gcgccattgc aggattagat ctcaatcaaa
     8941 tactccatca aaaccgttat cactttcaac ctcgaggtac gtcctctcga gtcgcacccc
     9001 cgcgaatcaa aaagggcacg cgcttttcgc gcgtgccctc catgggctag aaatccatgc
     9061 caccacccgg cattgccgga gcagcatctg cctttttcgg tttctcggcg atcatcgcct
     9121 cggtcgtgat cagcagaccg gctacggagg ccgcatcctg cagcgccgtg cgcaccacct
     9181 tcgtcggatc tatgatgccc ttggcaacga gatcgccgta ctcgcctgtt tgcgcgtcat
     9241 agccccaggc ataagagctg ttttccagca ccttgccgac gatgaccgag ccgtcctcgc
     9301 cggcattctg ggcaatctgg cgcgcgggag cctgaatggc ccggcggata atgttgatgc
     9361 ccgtcttctg gtcctcattt gccgggttca gtgaatcgag cgcctttagc gcccgcagca
     9421 gcgcgacgcc gccaccaggt acgatgcctt cctcgacggc ggcgcgggtc gcgttgaggg
     9481 cgtcgtcgac gcggtccttg cgctctttca cctcgacctc ggtggcgccg ccgaccttga
     9541 tcaccgcaac gccgccggcg agtttggcca gacgctcctg cagcttctca cggtcgtagt
     9601 cggaggtggt ggtctcgatc tgctgcttga tctggttgat gcgggcgtcg atgttctctt
     9661 tcttgccggc accgccgaca atggtggtgt tgtccttgtc gatggttacg cgcttggcgc
     9721 ggcccagcat gtcgagcgtg acgttctcga gcttgatgcc gagatcctcg gagacgacct
     9781 ggccgctggt caggatcgca atgtcctcca gcatggcctt gcgccggtcg ccgaagccag
     9841 gagctttcac ggccgcgacc ttcaagccgc cgcgcagctt gttgacgacg agcgtcgcca
     9901 gcgcctcgtt ctccacttcc tcggcgatga cgagcagcgg cttacccgtt tgcacgaccg
     9961 cctcgagcac cggcagcaac ggctgcaggc tcgacagctt cttctcgtgg atgagaacgt
    10021 agggatcgtc gaactcgacc gtcatctttt cggcgttagt aatgaaatat ggcgagatgt
    10081 agccacggtc gaactgcata ccctcgacca cgtcgagctc ggtctccagg ctcttggcct
    10141 cctcgaccgt gatcacgcct tccttgccga ctttctccat ggcctcggcg atcatcttgc
    10201 cgatttcgct gtcgccgttg gccgaaatgg tgcccacctg ggcgatctcg tcgttgttgg
    10261 tgacggcctt ggagttcttc ttcaggtcgg ccacgatggc gttgaccgca aggtcgacgc
    10321 cgcgcttcag gtccatcgga ttggcgcctg cggccacgga gcgggcaccc tcgcggacga
    10381 tggcctgggc gagaacggtg gcggtcgtgg tgccgtcgcc ggcgaggtcg ttggtcttgg
    10441 acgccacctc gcgcagcatc tgcgcgccca tgttttcgaa cttgtcgtcg agctcgattt
    10501 ccttggcgac ggtcacgccg tccttggtcg tgcgtggcgc accgaacgac ttctcgatga
    10561 ccacgttgcg acccttgggg ccaagcgtca ccttcacggc attggcgaga atgtcgacgc
    10621 cgcggagcat gccttcgcgg gcgtcatgag aaaaccttac ctctttggcg gacatctgct
    10681 ttcatcctcc ttcaatggcg ccttcagacg gcgccgaaga cgacagcgat caccagcgcg
    10741 agcgccatcg cggcgccggc tgcgctcaaa atctcagacc ttgtcacaag atttgatgga
    10801 tcaggctgcg gccttggtct tggcccggcc ttcgagaacg cccatgatgt cgctctcttt
    10861 catgatcaac agctcctcgc cgtcgatctt cacttcggtg ccggaccact ttccgaaaag
    10921 caccgtatca ccgggcttga catcgagtgg ggtgatcttg ccgttctcgt cgcgggtacc
    10981 gggcccgaca gcgagcacct c
//
LOCUS       DASVDU010000258         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1415711, whole genome shotgun sequence.
ACCESSION   DASVDU010000258 DASVDU010000000
VERSION     DASVDU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1415711"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF226_16940"
     CDS             <1..310
                     /locus_tag="VF226_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075726.1"
                     /translation="ADPAGSVVISTASPDQIRRYRFGLRTADYLLCATCGSYIGAVQA
                     EPDGRLISVINVGGLDVAEFSGVAADPVVYDGESIADRLARRRTYWMPAVVTEGPSRQ
                     "
     gene            397..1833
                     /locus_tag="VF226_16945"
     CDS             397..1833
                     /locus_tag="VF226_16945"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEX7075727.1"
                     /translation="MPTQILMPALSPTMEAGKLAKWLVKEGDKITAGKVVAEIETDKA
                     TMEVEAVDEGTVAKILVPDGTENVKVNTPIALIAGEGESVEEVSKAAAAAPAPQPAPQ
                     AAQPEPQAEPAPKPAAAAPQPTPSAAQAGPAVGGYGAAPTAQAQPTPARQGPAPQAEP
                     RTNGHDGRIFASPLARRLAKEAGIDLSLIRGSGPHGRIVKRDVEAAAATPGLGTAVAM
                     RPTAQPLPPSMAALQPMPDEKILALYDKNSYEIVPHDAMRRIIAERLTQAKLTIPHFY
                     LSVDCRIDELLKARERINASAPKEGPRAFRLSVNDFIIKAMALALQQVPAANATWTEQ
                     GTLRHKYSDVGVAVAIEGGLFTPVIRQAEMKSLSEISNEMKDLAERARRRRLAPHEYQ
                     GGSTSISNLGMYGVKSFDAVINPPHATILAVGSGEKRAIVVGDKIEIATMMSCTLSCD
                     HRVVDGALGAELLAAFKALIEDPVRMLV"
     gene            2125..2535
                     /locus_tag="VF226_16950"
     CDS             2125..2535
                     /locus_tag="VF226_16950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075728.1"
                     /translation="MLRAPTSMLIGAVLLAGCSGDGTFLGSPITTGSIQPQIDPACVS
                     LTAEIDGLMKEGIAEKVEKAAANKYRLKKADLAKADRLNKANAEFQNRCALNPAKPAS
                     TSAEAKPAEGQKEVAAASAKPAEASKAPAEQKSQ"
     gene            2732..3367
                     /locus_tag="VF226_16955"
     CDS             2732..3367
                     /locus_tag="VF226_16955"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7075729.1"
                     /translation="MKTVMVYGDSQTWGRKPGVPERMPFTRRWTSIMARRLGSEVHVI
                     VEALNGRTTCRDEPWKPWRNGAAHLDMLLACHAPLDLVIIALGCNDLQWHQHLGAYES
                     MLGVQVLLEKVLNWRGEPAGPPPRAFLLAPPRITKPAGPLAEKFRGAPEKSEEQQRLY
                     QQLAAELGVPFFSMAEVADPSPIDGIHLDEAGQAALGEAMAPRVAEVLGLN"
     gene            complement(3428..3862)
                     /locus_tag="VF226_16960"
     CDS             complement(3428..3862)
                     /locus_tag="VF226_16960"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7075730.1"
                     /translation="MGKLDINTATQEEIERIHGIGEARARQIVEYREQHGGIRHIDEL
                     LELPLFKIASEEDRRLLAESVTVNPETLPVSIGGKLNLNVADRDDLERIRGIGPQRAE
                     IILNHRRRYGHFRSLDEIDTLPSFDLMDKVELEAIKAHLTLE"
     gene            complement(4016..>4589)
                     /locus_tag="VF226_16965"
     CDS             complement(4016..>4589)
                     /locus_tag="VF226_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7075731.1"
                     /translation="AAVARAIERFGRIDILHNNVGTTHMDGPVELDEARFQAAVDINI
                     GSVYRTSKCVIPHMLRQGGGAIVNISSLAAIRWTGYPYFAYYATKAAVNHATVAIAMQ
                     YAKSGIRANCIMPGLINTPLIYKQIAGQYGSLEEMIEARNKLCPMGHMGEAWDIANAA
                     VFLASDEAKFITGVCLPVDGGQHCSVMAGA"
BASE COUNT          825 a         1445 c         1512 g          807 t
ORIGIN      
        1 cgccgatcca gcgggctcgg tggtgatctc gaccgcgtcg cctgatcaga tccgccgcta
       61 tcgctttggg ctgaggacgg cggattatct tctctgcgcc acttgcggca gctacatcgg
      121 cgcggtgcag gcggagcccg acggtcgact gatttcggtg atcaacgtcg gcggtctcga
      181 tgtggcggag ttctccggtg ttgcagctga tccggtcgtg tacgatgggg aaagcatcgc
      241 ggacaggctg gcgcggcgcc ggacgtactg gatgccggcg gtcgtcaccg aggggccgag
      301 ccgtcaatga ggcggtttca acctagtacg gattagtttg actgacttct atcgctggcc
      361 gctcatggcc gtagacaaca agggggccgc agatccatgc ccacccagat tttgatgccc
      421 gcgctttcgc cgaccatgga ggcgggcaag ctcgccaaat ggctcgtcaa ggaaggtgac
      481 aagatcaccg cgggcaaggt cgtcgcagag atcgagaccg acaaggcgac catggaggtc
      541 gaggctgtcg acgaggggac ggtcgccaag atcctcgtcc cggatggcac cgaaaatgtg
      601 aaggtgaaca caccgatcgc actcatcgcg ggggaagggg aatcggtcga ggaggtaagc
      661 aaggccgctg ccgctgctcc cgctccccag cctgcccccc aagcggccca accggagcca
      721 caggcagaac ccgcacccaa gccagcggcc gccgcaccgc agccgacgcc atccgcggct
      781 caggccgggc ctgctgtcgg tggttatgga gcggcgccaa cggctcaggc gcagccgaca
      841 cccgcgcgcc aaggccctgc accgcaggcc gagccgcgga cgaacggaca tgacggtcgg
      901 atctttgcct ccccgctcgc gcggcgcctt gcgaaagagg cagggatcga tctttcgcta
      961 atccgtggtt cgggcccgca cgggcgaatc gtcaagcgcg acgtcgaagc cgctgccgcc
     1021 actccggggc ttggaacggc ggtcgcgatg cgtccgacag cgcagccgct gccgccttcg
     1081 atggcggcgc tgcagccgat gccggacgag aaaatcctgg cgctctacga caagaactcc
     1141 tacgagatcg tgccgcatga cgcgatgcgc cgcatcattg cggagcggct gacgcaggcc
     1201 aagctgacga tcccgcactt ctacctgagc gtcgattgcc ggatcgacga gctgctgaag
     1261 gccagggagc gaatcaacgc atcggctccg aaggagggcc cgcgcgcgtt ccggctttcc
     1321 gtcaacgact tcatcatcaa ggccatggcg cttgcgctgc agcaggtgcc ggcggccaat
     1381 gccacgtgga cggagcaagg gacgcttcgc cacaagtatt ccgacgtcgg cgtcgcagtt
     1441 gccatcgagg gcggcctttt cacgccggtc atccggcaag ccgagatgaa gtcgctgtcg
     1501 gagatctcga acgagatgaa ggatctggcc gagcgtgccc gccggcgccg gctcgccccg
     1561 cacgagtacc agggcggctc gacctcaatc tcgaacctcg gcatgtatgg cgtgaagagc
     1621 ttcgatgccg tgatcaatcc tccgcacgcc accattctcg cggtcggcag cggcgagaag
     1681 cgggccatcg tggttggtga caagatcgaa atcgccacca tgatgagctg cacgttgtca
     1741 tgcgatcacc gcgtcgtcga cggagcgctc ggcgccgagc tgctcgccgc gttcaaggcg
     1801 ctgatcgaag acccggtgcg gatgctggtg tgaatctgag gcgactagtg agtagcgaat
     1861 ggcgaatggg gacttcgccg tctccacctg gcctattcgc cattggccat tcggtattcg
     1921 cccctctcca aaagtgtacc ctttgagaca cttgtaaccc gttctccaca gcgattgcgc
     1981 gggcgagccg gcctcgcctg tcggtaactc ggcatccgag cttgtcgggt tccgggtcgc
     2041 cgcataagca gaggcgcggg gacgattccg gtgagctgcg taacgtgcaa cggactctca
     2101 aacggggtat tcaagatgct taaaatgcta cgcgcgccga catccatgct catcggtgcc
     2161 gtgcttctcg ctggttgctc gggtgacggc acgttcctgg gcagcccgat cacgacgggc
     2221 tccatccagc cgcagatcga tccggcttgc gtcagcctca cggccgagat cgacggcctc
     2281 atgaaggagg gcatcgcgga aaaggtcgaa aaggccgcgg ccaacaagta ccgtctgaag
     2341 aaggctgatc tcgccaaggc cgatcgcctc aacaaggcca atgccgagtt ccagaaccgc
     2401 tgcgccctga atccggccaa gccggcctcg acgagcgccg aggcaaagcc cgccgaaggc
     2461 cagaaggaag tcgccgccgc cagcgcgaag cctgccgagg cctccaaggc gccagctgag
     2521 cagaagagcc agtaggccct gaggagaggt ctgccagtgc gcctattccg aagttcgcca
     2581 gagcctgcgg gttcgcgagc gggattcgga gccgaagtta tgagaagcgt aggatcggaa
     2641 tttctcattc gatgcattcc gcgaccggca gcggatttct agttcgggcg ggcggcgtcg
     2701 gccgcccgtt cgcgattgag gagttggcgt gatgaagacc gtcatggtct atggcgacag
     2761 ccagacctgg ggccgcaagc cgggtgtgcc tgagcgcatg cccttcacgc gacgctggac
     2821 gagcatcatg gcccgccggc tcggcagtga ggtgcatgtc atcgtcgagg cgctgaacgg
     2881 ccggacaacg tgccgggacg agccctggaa gccgtggcgc aacggtgccg cgcacctcga
     2941 catgctgctc gcctgtcacg caccgctgga cctcgtcatc atcgcgctcg gctgcaacga
     3001 tctgcagtgg caccagcact tgggtgctta cgaatcgatg ctcggggtgc aggttctgct
     3061 ggaaaaggtg ctcaactggc gcggtgagcc ggccggtccg cctccgcgcg ccttcttgct
     3121 ggcgccgccg cgaatcacga aaccggccgg ccctctcgcg gagaaattcc gtggcgcgcc
     3181 ggaaaaatcg gaggagcagc aacgcctcta tcagcagctc gctgccgagc ttggcgttcc
     3241 attcttttcg atggcagagg tggccgatcc gagcccgata gatggaatcc atctcgacga
     3301 ggcgggccaa gctgctttgg gggaggcgat ggcgccgcgt gtcgccgagg tgctcggcct
     3361 caactgacca cgtctattcg tcgatgacgt cgcttactgc gcgggcgggc agggagccgg
     3421 cgcgggctca ctcgagcgtc aggtgcgcct tgatggcctc cagttcgacc ttatccatga
     3481 ggtcgaagct cggcagcgtg tcgatctcgt ccaggctgcg gaagtggcca tagcggcggc
     3541 ggtggttgag aataatttcg gcccgctgcg ggccgatgcc cctgatccgc tcgagatcgt
     3601 cacgatcggc gacgttgaga ttgagcttgc ccccgatgct gaccggcagg gtttcgggat
     3661 tgacggttac gctctccgcg agaagtctcc tgtcctcctc agaagcaatc ttgaacagtg
     3721 gcagctcaag cagctcatcg atgtggcgga tgcccccgtg ttgctctcga tactccacga
     3781 tttgcctggc gcgtgcctcc ccgatgccgt gaatgcgctc gatctcttcc tgggtcgcgg
     3841 tgttgatgtc gagcttgccc atcgctgtgc tcatcctctc gggccggcag gcgacgccgc
     3901 ggagccgctc acatccatac aacattacgg atgccgggga cggcttcgca cctcctccac
     3961 gcggcatcaa cgatcgctgc gggcggcggt tccggggatg agctctgggg ggcgatcagg
     4021 cgcccgccat gacggagcag tgctgcccgc cgtcgaccgg caggcagacg cccgtgatga
     4081 acttcgcctc gtcggacgcc aggaaaacgg cagcgttggc gatgtcccag gcctcgccca
     4141 tatgccccat cgggcagagc ttgtttcgcg cctcgatcat ctcctcgagc gagccgtact
     4201 gaccggcgat ctgcttatag atgagtggcg tgttgatgag acccggcatg atgcagtttg
     4261 cgcggatgcc cgactttgca tactgcatcg cgatcgccac cgtggcgtga tttactgcgg
     4321 ccttggtggc gtagtaggcg aagtacggat acccggtcca gcggatggcg gcgagcgagg
     4381 agatgttgac gatcgccccg ccgccctgcc gcagcatatg cgggatcacg catttcgacg
     4441 tgcgatagac ggacccgatg ttgatgtcga cagccgcctg aaaacgggcc tcgtcgagct
     4501 cgaccgggcc gtccatatgc gtcgtgccga cattgttgtg cagaatgtcg atgcggccga
     4561 accgctcgat tgcgcgtgcg acggcagcc
//
LOCUS       DASVDU010000259        33255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_88284, whole genome shotgun sequence.
ACCESSION   DASVDU010000259 DASVDU010000000
VERSION     DASVDU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 33255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33255
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88284"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(517..1260)
                     /locus_tag="VF226_16970"
     CDS             complement(517..1260)
                     /locus_tag="VF226_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEX7075732.1"
                     /translation="MRQGQQNRRGRGRNRKAHHNPLTRNYESNGPDVKIRGTASHIAE
                     KYMSLARDALASGDPVLAENYLQHAEHYNRIIMAFREQTIQQSGEAMNGAGRHPGEPE
                     DFSDEDGDDGESYLLPPRGHELQQRLDGQQPRLDGQQQRFDERSRRPGLDQSGRPMQE
                     RYQESRNGNGAKAERNGHQPQAAEPPQVDEAAQQSLTEAPQRRRDRFGGAGQDQPEFL
                     KRPVRRARRDTNGGAGEASSVAAKDDSGE"
     gene            complement(1715..2620)
                     /gene="prmC"
                     /locus_tag="VF226_16975"
     CDS             complement(1715..2620)
                     /gene="prmC"
                     /locus_tag="VF226_16975"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7075733.1"
                     /translation="MPTTISDNELHRLTAAAAIKETTRRLRGSGIESAARDARLLVAA
                     ALGCDSERLISQPEARLDATQTDLLHSFVQRRLAREPVSRILGMRGFYGRDFEISPAV
                     LDPRPDSETLIEAALDLVGAQDGAETAPLRILDIGTGSGCLLVTLLAELPHATGLGTD
                     IDPQALDIAQRNARRHGVQSRASWMYARSLNGIGGPFDLVVANPPYVPTGIIETLEPE
                     VRDFDPRSALDGGADGLEIYREIAADLARVAPNGWAVFEVGKGQSDAVSELLSSIRVR
                     NEAPLVRVFRDLNGVERCVAWRALK"
     gene            complement(2601..3689)
                     /gene="prfA"
                     /locus_tag="VF226_16980"
     CDS             complement(2601..3689)
                     /gene="prfA"
                     /locus_tag="VF226_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX7075734.1"
                     /translation="MNSVPQEKLEKLIQRWDVIQAELSQGVNQATYAKLTKEFSDLKP
                     IVTTVQELRKAEKELAELTAIAEDAGSDREMAALAEQELAALRPKIEELEQKLRILLL
                     PKDAADDRSVILEVRAGTGGDEAALFAADLFRMYQRYADLHGWKTEIISMSENDLGGI
                     KEVIASISGDGVFARLKFESGVHRVQRVPETEASGRIHTSAATVAVLPEPEEVDVEIK
                     PEDIRIDTMRAGGAGGQHVNKTDSAVRITHLPTGIVVVSAEKSQHQNRRLAMQVLRSR
                     IYERERQRTADERAAQRRGQVGSGDRSQRIRTYNFPQGRVTDHRINLTLHKLDTILDG
                     SALDELIDALITDHQASQLAEANAHNHI"
     gene            complement(3861..6155)
                     /gene="ptsP"
                     /locus_tag="VF226_16985"
     CDS             complement(3861..6155)
                     /gene="ptsP"
                     /locus_tag="VF226_16985"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX7075735.1"
                     /translation="MQTRRGDEPRTLPSEPALRVILRRLREIMAEPGDGQSRLDKIVR
                     QISGLMVAEVCSIYLKRQDGSLELFATEGLKKEAVHNTRMKRGEGLVGRCAEIAVPIN
                     EPDAQLHPAFSYRPETGEEIYHSMLAVPILRGGVVLGVLAVQNRTRREYSDEDVEVLL
                     TTAMVVAEHIVSGAVAGVNAAAEISRSLAGVAHGEPISEGIALGHVVLHEPRVVVTKL
                     LAEDVATELKRLESAVHRLRASLDEMLSAEALAQAGDHRDVLEAYRMFANDRGWMRRM
                     EAAVREGLTAEAAVERVQNATRTRMLRQNDPYWRERLRDLDDLSDRLLRILAGRTTAA
                     REGLELPADTILVARTMGPAELLDYDREKLRGLVIEEGSGQSHVAIVARALGLAAVGQ
                     ARGIVELVSTGDPIIVDAERGDIHVRPSTDIVTAYSDKVRFRARRQRRYQALRELPTV
                     TKDGARIHLHINAGLLVDLPHLHESGAEGIGLFRTELQFMISSTFPRRDKQTETYRQI
                     LDGASGKPVVFRSLDIGGDKILPYIRHPKEENPALGWRAVRISLDRPGLFRTQIQALI
                     RAAGGRELSIMVPMVSTVSEIEAVKALIAREVDHLAKRGVPRPERLRLGAMVEVPSLL
                     YELDALMPQVDFVSVGSNDLMQFLFAADRSNAQVGHRYDPLSVSALRALKQVVRAGAR
                     HGVPVTLCGEMAGRTLDAMALIGLGYRTISMAPASIGPVKSMILSLDAAKVASVVDEL
                     LETKSGSIRSYLQDFAKAEGVELA"
     gene            complement(6271..7536)
                     /locus_tag="VF226_16990"
     CDS             complement(6271..7536)
                     /locus_tag="VF226_16990"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7075736.1"
                     /translation="MAVVVMKFGGTSVADINRIRNVARHVKREVDAKNQAAVVVSAMA
                     GHTNQLVAWVREMSLIHDAREYDAVVASGEQVTAGLLAIALQSIGVPARSWQGWQIPI
                     LTDAAHGAARIQEIQADEIRQRLAQGEVAVVTGFQGLEPQRNRISTLGRGGSDCSAVA
                     LAIALNADVCDIYTDVDGVYTTDPRIVPTARRLPRISYEEMLEMASLGSKVLQTRSVE
                     LAMVHRMRTRVLSSFADAETIEPVRGGAEWQNIGTIVCDEEEIVESQVVSGIAYAKDE
                     AKVTIRRVADKPGVAASIFGPLADASVNVDMIVQNLSSDGKHTDMTFTVAASELPKTL
                     EVLRKCQETIGYSEIDSSSDVVKVSVIGVGMRSHAGVAAQMFKALAEKGINILAITTS
                     EIKISVLIDAAYAELAVRTLHTLYGLDKD"
     gene            7733..8494
                     /gene="ubiG"
                     /locus_tag="VF226_16995"
     CDS             7733..8494
                     /gene="ubiG"
                     /locus_tag="VF226_16995"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX7075737.1"
                     /translation="MTSAHPSQATLDPEEIDRFARLAGKWWDPEGEFRPLHKLGPARL
                     SFIKSHLVRRFGLPERGLKPLAGLTILDIGCGGGLISEPLARLGGTVTGIDPATENIA
                     TAIQHAEPQGLVIDYRTARAEDLVEERTQFDAVVCLEVVEHVPDPQAFIRTCAALVRP
                     GGLFITSTINRTLKAFALAIVGAEYILRWLPVGTHQWDRFVTPEELARYCQAAGLQQP
                     VFEGLIYNPLTGRWSLGQDTDVNYMAAADKPGSTS"
     gene            complement(8517..9344)
                     /locus_tag="VF226_17000"
     CDS             complement(8517..9344)
                     /locus_tag="VF226_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7075738.1"
                     /translation="MTEIDYEAEYNNRRRVPEHTEIMARWAAHSARVRATLAHELDLS
                     YGSGPRNRFDLFAAPEGTADGPLVAYIHGGYWQRGDRVDYSFVAEALVGRGAVVALPS
                     YTLCPEATIAQIVDEMRQFLVALWRRTAKRPVIVGHSAGGHLAAAMMATDWSRHDAPA
                     DLVRAGYAISGVFDLTPLIPTSLNEALRLTPEAAREASPILWPPPPRDRTFVAAVGGA
                     ESQEFLRQSLDITAAWSAAGVKAECVVVPNANHFTVVDELTRPESAMVQRILQLAQS"
     gene            complement(9356..10987)
                     /locus_tag="VF226_17005"
     CDS             complement(9356..10987)
                     /locus_tag="VF226_17005"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX7075739.1"
                     /translation="MESDGGAETFDYVIIGAGSAGCVLANRLSADPGVRVALLEAGGR
                     DLNPWLHVPAGYFRTMFNPKLTWQFGAGPEPYLNGRIVPWPRGRVLGGTSAVNGLLYV
                     RGQAQDFDTWRQLGNAGWSYRDVLPYFKRAENQERGADEYHGAGGPLGVSDVRMKNPL
                     CEAYIAACVAAGIPRTTDFNGASQEGVGYYQLTTWKGRRCSTAVGYLNPVRSRKNLAI
                     ITNAEVKRISLDGRRATGVVYKRDGQTRTVAAAREVLLAAGAIGSPQLLQVSGIGPGA
                     VLKNAGVEVLHELPGVGENLQDHFQARFVYECSLPASLNDVWHSRLMQVRAGLEYAFS
                     RSGILTIGAGVVGVFARSRPELDVPDVQVHFMPLSAEGPGQGLHPFSGVTASVCQLRP
                     ESRGVISITSPDPAAPPSIVANYLASQTDCDVLLAGIRMLRRIAEQPAFKQYVKREFL
                     PGPEVESDEDLMAFAKAKGTTIFHPCGTCKMGRDPMAVVDERLRVHGIAGLRVVDASI
                     MPTMTSGNTNAPTIMIAEKASDMIREDARQGAQAA"
     gene            complement(11039..11947)
                     /locus_tag="VF226_17010"
     CDS             complement(11039..11947)
                     /locus_tag="VF226_17010"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075740.1"
                     /translation="MPTSKWAKMPETIAFIGLGAMGLPMAQNLVRKQFRVVGYDIRAE
                     AVANLVEAGGHGAKSAREAADGADHLILMVVNADQAEHVLFEQGTLDVLASDATVILM
                     ATCPPGRVEAMAKRVADTGRSFVDAPVSGGVAGATAGTLTIMVGAAQPAFTCSKPILD
                     AMGTNIYHLGEEPGQGAVMKTINQLLCGVHIATAAEGLALAEQAGVDPKVAQEILSGS
                     AASSWMLQNRGPRMVADDGQVTSALDIFVKDLGIVLEAGRSVKMGLPLAAAAHQLFVA
                     ASGLGLGNRDDSQVIEIYRALRPKES"
     gene            complement(12037..13128)
                     /gene="ugpC"
                     /locus_tag="VF226_17015"
     CDS             complement(12037..13128)
                     /gene="ugpC"
                     /locus_tag="VF226_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7075741.1"
                     /translation="MAQVVLKQINKFYDSVHAVKDVNLTIHDKEFMVFVGPSGCGKTT
                     TLRMVAGLEATSSGNILIGEQIVNDLPPMDRDIAMVFQNYALYPHMSVFDNMAFGLKM
                     RKFDKAEIRQRVKQAADILGIEQLLDRKPRQLSGGQRQRVALGRAIVRNPQVFLFDEP
                     LSNLDAKLRVQMRVELKKLHDRLGTTAIYVTHDQVEAMTLGDRVVVMRDGWVQQVGEP
                     LELYNSPANKFVAGFIGSPAMNFCNVTVQDEAGELWLQNSGMRLRVPDGSAARLRSHV
                     GREVTLGMRPEDLRVANSADPAGCCFDAVVEVVEQLGSEILLDVSVGNATMVAAVDPS
                     LRTKVDEKLRLAVNPDRLHFFDVETEAAI"
     gene            complement(13157..14008)
                     /locus_tag="VF226_17020"
     CDS             complement(13157..14008)
                     /locus_tag="VF226_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7075742.1"
                     /translation="MIEGAVWRRWVYFRLPLLLFILVLLFPFYWMLITSFRPDGELYR
                     PWNAVNYNPFWTWKPTLAHVRYLLQETMFLKWLWNTMFIAIVSTVISLICGVFAGYAL
                     ARLNFRFAGFLGTGIFITYLVPQTLLFIPLSEIIRNYRLGDTPWSLILTYPTFLIPFC
                     TWLMMGYFKAIPKELEECARIDGASRWQAMLYIVVPVAVPGILSAGIFAFTLSWNEFI
                     YALVFLSSPEQKTVPVGVVSELIRGDVFFWGPLMAGALLGSIPVALVYSFFVEHYVSG
                     LTGSVKG"
     gene            complement(14013..14939)
                     /locus_tag="VF226_17025"
     CDS             complement(14013..14939)
                     /locus_tag="VF226_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7075743.1"
                     /translation="MAILTADTVAAKTPPRRRLLEDERWLALVLLLPTAILLGLFIAY
                     PFFRGIILAVTNARVGVPGQFVGLENFQRLAGDPIFHRVVWNTVWYTAVTTVFKLGLG
                     LWLALLLNRSFKGKAYVRAFILLPFIIPTVLSTFAWKWMFDPTFSVLNWLMYHLSIID
                     RRINWLGDGDLAMISIIIVNIWRGVPFFAISLLAGLQTISPDLNEAAAIDGAKPLQRF
                     WYITWPLLLPVTMVVVLFSVIQTFADFQIVYVMTGGGPANATHLFATYAYQLGVGTGL
                     LSQGAAVSLAMFPILFLIVVVQLLYIRRVEIR"
     gene            complement(15046..16371)
                     /locus_tag="VF226_17030"
     CDS             complement(15046..16371)
                     /locus_tag="VF226_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7075744.1"
                     /translation="MARVHRRDFLKLSGAGAITAKAGGMAAILASGKAPAYAQTTTVH
                     WLRWNDFVPACDQLLRQKLLPEAEKALGIKVNFETVNGNDLQPRITSAIQAGSGPDII
                     MLFNNHPHLYKASLVDLNDVADVIGKEQGGFYDISKGNCSADGKWMGIPMAIIGAMNA
                     YRKSAFAEVGYNEFPKTWDEYRDAGKKLKAKGLPIGQALGQSFGDPPTFAYPLMWAYG
                     GVEIDESGKVALDSKETVEAVKYLTAAWKDAYDEGGLAWDDSNNNRAFLSGTICSTLN
                     GGSIYVEAVRKAEQYQTADGKPMKDDIRHAPLPAGPKGQFGFHTFTSHVMPSYSKNQS
                     AAKELLKFVHAKQNYEQWFTTAKGYYTPGTASWETHKMWDENPVMKPFAIAGKLGQTP
                     GYKGPPNAKAAEVLSKYLIVNMFAEAVKGASAEDIVKQTHAELKKIYES"
     gene            16668..17510
                     /locus_tag="VF226_17035"
     CDS             16668..17510
                     /locus_tag="VF226_17035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7075745.1"
                     /translation="MYESAVATLSREAVVFRTPCGSGDLVWRRWGTGAPVVLCHGGSG
                     SWTHWIRTIPALSRRYELWVPDLPGLGDSAMPPKPWTPATSASILTAGLRQLVSPDRK
                     VHLVGFSFGAHVATLAAVELGDWLADFTIIGSAALGLPWHSLELPKERSTMSAEERRA
                     VHRRTLEILMFADPRRIDEEAIAIQMENIAKARFRSREFAATDEIRTTLARVTAPLRA
                     IWGERDAIARPSVEACFDVLRLYHPELVTRTIPGAGHWVMYEAPDAFNAALMELLELP
                     PNRA"
     gene            complement(17523..17987)
                     /locus_tag="VF226_17040"
     CDS             complement(17523..17987)
                     /locus_tag="VF226_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX7075746.1"
                     /translation="MPASISLELAERIVDGALTAASRIGTAPMAAAVLDAGGHLVAFK
                     RQDGLAFLRCEIAQAKAWTALAMGTSSRGSRDRLAERPTFQAALSALSQGKFIPVPGG
                     VLILDADQRVIGAVGMSGDASDKDEACCIEAIRAAGLSSYPAEPAPGWDAKQ"
     gene            complement(18023..18706)
                     /locus_tag="VF226_17045"
     CDS             complement(18023..18706)
                     /locus_tag="VF226_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7075747.1"
                     /translation="MPFLFDPPPVPALPIIGSDKLFPVHRIYCVGRNYAEHTREMGFD
                     PDKEPPFFFMKAPDTLVTDGRFPYPSASQDVHWEIELVVALKSGGVNIPRAQAMSHVY
                     GYAVALDMTRRDLQGMAKKMGRPWEIGKSFEKSAPCSAIVPAEKIGHPTTGAIWLDVN
                     GERRQSGDLSQMIWDIPSQIEYLSGLWELKAGDLILTGTPAGVGAVKRGDRLLGHVDG
                     VGNLEVEVV"
     gene            18925..19842
                     /locus_tag="VF226_17050"
     CDS             18925..19842
                     /locus_tag="VF226_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX7075748.1"
                     /translation="MFLRIDRLQVEMPLPKDPDPAAAAAVNELMGGRFGEMTTLNTYM
                     YQSFNFRMRDKLRPFYALIANIATEELGHIELVGAAVNGLLYGADGKGSVAAGDAPFE
                     DMFKQAGAGANYHINGPHGILAANSLGQPWRGDYVFNSGNLVLDLLHNFFLECGARTV
                     KLRVYEMTKNPAARMMLGYLFTRGGVHAHAYALALEKLTGVEVRKMLPIPRILDAEIP
                     ECDKFIKEGSHRKLYRFSPDDYKEIAAIWNGPGLDASEILEVVDGPPQGGPQYQMDGV
                     PEAFIPEYHPEEIVDIAKKLYTAAGGSQP"
     gene            complement(19945..21045)
                     /locus_tag="VF226_17055"
     CDS             complement(19945..21045)
                     /locus_tag="VF226_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7075749.1"
                     /translation="MAEVTSREHFWRIAIVVALILATLFVGERYLRVFVFAATEPRET
                     TPRPDLTPGEQIATRVYAETAPSVAYIFTHQATQRPGGRALERAGAGSGFVWDRAGHI
                     VTNTHVVEGADEVGVVINQGKVHPARVVGTAPWVDLAVLQLIDPPPDLQPIKVGTSAD
                     LVVGQMVFAIGNPFGLSRTLTSGIISALDRTLPTESGREIAGVIQTDAAINPGNSGGP
                     LVDSAGRLIGVNTAILAPSGTFAGVGFAIPVDTVNRIVPELIRTGRAPLPGIGIRVFP
                     VELTARLGAPGVVIQGVTPGSAADRAGLKGMDELGRLGDIIIGVNGKRINELSDLTRE
                     LEKAGIGNTVTLTVLRNGSERDVQVQVEDINK"
     gene            complement(21197..22114)
                     /locus_tag="VF226_17060"
     CDS             complement(21197..22114)
                     /locus_tag="VF226_17060"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075750.1"
                     /translation="MSARDETIGFIGLGAMGRGMAANCVKKGFKLVVHDLRPEPVKEL
                     VSLGATAGKDIADVARQCSIIITVLPAGKEVTEIVMAPGGIFDHAKPGTLVMDLSTVE
                     PETTDRLHAEAKQRGFRAVDAPIGRLAWHAERGESLFMVGAEPEDFERVKPLLEAMGT
                     TIHHCGPGGTGTRTKLVNNYLAIISCQLNAEALALSQRFGLDLEKTLEVIHGTTATNG
                     QLKINWPNKVLAGDTTPGFTIDLAHKDLSLIVGAANAARLPVPIAAAAREAFSSARAR
                     GYGNQDFSAMVDVLCDLVQVERARLKKQS"
     gene            complement(22218..25676)
                     /locus_tag="VF226_17065"
     CDS             complement(22218..25676)
                     /locus_tag="VF226_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002963656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7075751.1"
                     /translation="MHIQRLRLLGFKSFVEPTDLLIEKGLTGVVGPNGCGKSNLLEAL
                     RWVMGETSHKSMRASAMEDVIFAGTTSRPARNSAEVTIFLDNRQRKAPAEFNDSDTIE
                     ITRRIERDAGSTYRINGREVRARDVKILFEDAATGARSPSLVRQGQIGEIVNAKPEQR
                     RRILEDAAGIAGLHSRRHEAELRLKAAEANLARLADILGQVSAQMESLKRQTRQVKRY
                     KELSSEIRRAEAMLLYLQWVEAQAQVEAEEAALTEAMARLGVAVKEESELVRREAELA
                     QSLQPLRDEEATRAAVLSRIKVQQETFEREAERMKERELELKGRIAQLASDLEREDGL
                     IREARHLISQLEQEEHALKGAIEQAVEQEEAARDHLASAQSELERIEATLQDATARTY
                     ESRARKQSLTAALTERRDEVAKLERQLESLAAQARDIAAKAPDTTRLAELQELVRELT
                     LETAELETNAVAAEERVAAAAEKARRTREASRQAELVARQLTTELATLSKLLTPAQST
                     PYPAVVDLLEVAPGYEQALGAALGEDLDAPIAPEAAAHWSLIEPAPDDAPLPEGAEPL
                     AQAVDGPPELTRRLLQIGIVPRELGARLQPALKPGQRLVSREGDLWRWDGFVAAADAP
                     TPAAQRLAERNRLVELRHKEAAAREAAAAAASEAVAAAERQRKAEEDERRLRQAWRET
                     QSRLVKLRDQLNALERAAREAEGRIAAVREAKARAEASVKEARARLVEVENELSDLSS
                     GEDYEPLMASAQQQARIARERVAQLRTALAALERERQMRAERKTKAAAERERWAQRAA
                     SAERQIGTLQARLAEAKAELDELAQLPPLIEEKRQKLLNTLAEAERERKAAADRLAAA
                     EAAQREAAQALRAAQATVSTERESRARVEARLEAVRQRRSEEVARIRDTFGVAPEGCL
                     DIAGIKPGAPLPKLADVDRVLSRLKADRERLGGVNLQAEEELQSMQEHYSKLDAERAD
                     VEAAIARLRGGIGQLNREARKRLQNAFDLVNGHFQRLFTTLFGGGEARLEMIESEDPL
                     EGGLEIIAKPPGKKPATLSLLSGGEQTLTALSLIFAVFLTNPSPICVLDEVDAPLDDA
                     NVDRFCTLMERMAEETETRFLVITHHPMTMARMNRLFGVTMAEKGVSQLVSVDLANAE
                     RFLEAV"
     gene            complement(25737..26531)
                     /locus_tag="VF226_17070"
     CDS             complement(25737..26531)
                     /locus_tag="VF226_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7075752.1"
                     /translation="MLPSISRQSAAAGVLLAAFALAACSGETSLGEALQATGAVPAGS
                     GASIDQPEFNPFGDAVSTPKPARQVIQNPSLAEVLKPGPLPEMALGRDDAPVTIVEYA
                     SLTCPYCRKFHLETFPKLKREYIDTGKVRFILREFPIGLQSGAATIALRCAPQERYFE
                     LFGKFLEQQARWVSQEVRHEPIFKIAAQVGMTRPQFDACYENQGMIDALKAIKDRGRT
                     LGVIGTPNFFINNRLVKTVIGMEEIREMVEPMLANSSGPGKVTATR"
     gene            complement(26538..27140)
                     /locus_tag="VF226_17075"
     CDS             complement(26538..27140)
                     /locus_tag="VF226_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7075753.1"
                     /translation="MATAPFITAALAQRRGPSEVPVEELMKPGPLPDLVLGNPDAKIT
                     IVEYASMTCPHCANFNNRVLPELKKKYIDTGQARLVFREFPLDDLAAAGSMLARCAGD
                     KALPMVDVLFEKQDEWVTRNDPVSKLFEIAKQAGFTKESFEKCLGDDKLLADIEATRR
                     RANEQFGVNSTPTFFINGKRLSGGNSISDFDKAIEPLLKS"
     gene            complement(27314..27931)
                     /locus_tag="VF226_17080"
     CDS             complement(27314..27931)
                     /locus_tag="VF226_17080"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX7075754.1"
                     /translation="MNSRTSRAQPLPPRAVLNPRMRAQPSARAVSSYIPQLTRKAFEK
                     YGFASLALLTDWANIVGSDIASFTAPERLKWPRGVDAFDETEPGNERRPGATLVLRVD
                     GPRAIELQHKTHQIIERINASFGYRAVAELRFVQAPIAGQSVPKWRPPVLPQDDAAAG
                     ETPPELAKIENPALRHALARLQASMHRERPGSPASKAAAQPLTQP"
     gene            28040..29119
                     /gene="mutY"
                     /locus_tag="VF226_17085"
     CDS             28040..29119
                     /gene="mutY"
                     /locus_tag="VF226_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7075755.1"
                     /translation="MQPERDRDAAAALLEWYDAERRDLPWRSPPGVRPDPYRVWLSEI
                     MLQQTTVKAVITYFDRFLSRWPTVEQLAAADLDDVLAEWAGLGYYSRARNLHRCARLI
                     VERYDGRFPSSEAELLKLPGIGPYTAAAIAAIAFDQRATPVDGNVERVVARLFAVTEP
                     VPPAKPLLRELAAKLTPNRRPGDFAQAMMDLGATICTPKRPSCLMCPLNSQCLAKARG
                     VQGELPVKMDKGERPIRFGVAFLALREDGCVLLRKRPETGLLARMMEVPSTDWLEDPR
                     VVSEALRAAPVRADWWRVPGQVAHTFTHFRLELIVYRAVVESDSTLTFWADANRCRWV
                     ARRELDRQALPSLMRKVIAHGLREM"
     gene            complement(29171..31195)
                     /locus_tag="VF226_17090"
     CDS             complement(29171..31195)
                     /locus_tag="VF226_17090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7075756.1"
                     /translation="MLNTQRHAPVGRARAIRLPSRTQRLPHVYRGRARRAVPDIYDRE
                     FNEPRRGGRFRWVLSTFLAAGVGAVAIGVVLFGSLDRMDTSGGVIPALQQFREARLPQ
                     PAIVRDSGLNWSIPKSDRLQVAVGSVGAKHIIHEQVQVRRDNRPFIQIRPYMRIVARL
                     SEVPPDKIEEIPPFNPLKLYAAEAESSEADGASGEGRSDVSVRVVELLGGILPGEDGQ
                     ELDTAEVLELVARAEEAAQNLAIRPSLQPDDPVAEQEAEPDLAAGDAPAAEDAPNTTV
                     LFKSVSDPDDAEDDLEASEVRVIRVARGDSLARILQRLGAEPWQINAMLQAARPTFPD
                     NALMPGQEVHVTLVPSLSDPQQMEPVRFSIFAEGHEHKVTVVRNTAGEFVASAQPFND
                     DKFARATLNDSDKAHSASLYSSIYYAALSEGVPPEKIMQILRVHAYETDFRRRVGHGD
                     MVDFFFDVKEDDGPDSTPGELLYTSITSGGEQQRFWRFRTPDGIVDYYDEHGNNSKRF
                     LMRRPVRGDFVRLTSGYGMRLHPVINVARMHTGIDWAAPVGTPILAAGNGVVEEAHRK
                     GGYGNYVRIRHANSYQTAYGHMSRIAPGIKEGVKVKQGQIIGYVGATGLASGPHLHYE
                     ILVNSRFVNPLQIQVPRERQLTGRQLAEFQKERARVDELMRRAPVMTASR"
     gene            32163..>33255
                     /locus_tag="VF226_17095"
     rRNA            32163..>33255
                     /locus_tag="VF226_17095"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         5954 a        10582 c        10384 g         6335 t
ORIGIN      
        1 acaaagccct tggaccggtc cgtgtactta tcgaaggtca taagtctctc ctcagcgcgc
       61 ccgctcgccc aaatcggcac gaatcgaacg ccgttccgtc aggaatgata cgacgtcgtc
      121 ggcaggcccc tacaggcacc ctccgacccg ccagcagcag atatagggtc gctgcggcgc
      181 ggcgaaaggg gcaggccggc cctcaactca gcacatcttg caggagataa acttgcacca
      241 ggtcaaaaag tcagcggctc cgggccaccc aaccgatgga gcccggtgag cgagcgcaca
      301 cgaccccacg cccccggcgg ggatgcggcg cgcctggggg caattgaatc tggaacttga
      361 gcaaacgcca tcggagccgg ccacgtcgcc accggcactt ccgcattctg gctcggctac
      421 tgattctcag acgcccgtct cgatcggcgg tcgtcaaatc ccccggacgg cgctatcggt
      481 gccgggttac ctcgccggct tgccccgaag cgtcaattac tcccccgaat cgtccttggc
      541 ggcaacgctg ctcgcctcac ctgcgccacc attcgtgtcg cgtcgagccc gccgcacagg
      601 ccgcttcagg aactcaggct ggtcctgtcc agcgcctccg aagcggtcac gccgacgctg
      661 cggagcttcg gtgagggact gctgagcagc ttcgtcgacc tgcggcggct cggctgcctg
      721 cggctgatgg ccattccgct ccgctttcgc accgttaccg ttgcggcttt cctggtagcg
      781 ctcctgcatg ggtcggccgc tctggtcgag ccccgggcgg cgtgaccgct cgtcaaagcg
      841 ctgctgttgt ccatccagcc gcggttgctg cccatccagc cgctgttgca gctcgtggcc
      901 gcgtggcggc agaagatagc tttcgccatc gtcgccatcc tcgtcggaaa agtcttccgg
      961 ttctcccggg tggcggccgg caccattcat ggcttcgccg gactgctgaa tcgtctgctc
     1021 ccggaacgcc atgatgatgc ggttatagtg ctccgcgtgc tggagataat tttcggccag
     1081 gacagggtcg ccggaagcca gcgcatcccg tgccaaggac atgtattttt cggcaatgtg
     1141 cgacgccgtc ccccggatct tcacatccgg accattcgat tcgtagttac gcgtcagcgg
     1201 attgtggtgg gccttgcggt tacggccgcg accccgccgg ttctgctgtc cctgcctcat
     1261 agtctcccaa tctcaactat gcagataact tctagttttg gtttgcttta cgaggggcac
     1321 tggcagcacc gctgccagat gcatctgctg caacagacgt gcaccatcgc cccccacgaa
     1381 gcagcgcgcg aaaaggcatc gctgcaatcg ttacgtcact cgcgcgcgca atcgcgccgg
     1441 gtaaattctt gtgctgttga cattcacgaa gcttcgccag tcaaccaatc gggaaaaggc
     1501 gcattagcga aacaccgccg ccaccgtggc cgctggtccg accgaccaac tggcaaccag
     1561 cacgtccacg ccgctcgttt cgctcggtct acttgcgctc gcaggtgaca gccgcaatga
     1621 gaacctcggc tgtactgttg cgctcacgct tctttccgcc tggcccgaca ttagcttgtg
     1681 accggttcgt ttccaagcat ttttctctcc gcgatcactt gagtgccctc caggccacac
     1741 accgctccac cccgttgagg tctcggaata cacggacgag aggcgcttcg tttcgaactc
     1801 ggatggaact cagcaattcg cttaccgcgt cggattgacc tttcccgacc tcgaacacgg
     1861 cccagccatt cggcgcgacc cgggccagat ctgctgcgat ctcgcgatag atctccagtc
     1921 cgtcggctcc gccgtcgagt gcggatcgcg gatcgaaatc gcgaacctcc ggctccaatg
     1981 tctctataat acctgtcgga acatacggcg gatttgcgac caccaggtca aagggcccgc
     2041 cgataccatt cagagagcgt gcatacatcc aagaggcacg cgattgaact ccatggcgac
     2101 gtgcattgcg ctgcgcaatg tccaacgcct gcgggtcgat gtcggttccg agtccggttg
     2161 cgtgggggag ctctgcgagc aacgtcacca acagacagcc agatccagtt ccgatgtcga
     2221 gaatccgaag cggggcagtc tccgcgccgt cttgagcgcc aaccaagtca agtgcagctt
     2281 cgataagcgt ctcgctatcc ggcctcgggt cgagcaccgc gggagagatc tcgaaatccc
     2341 ggccgtagaa cccgcgcatt ccaagaatgc gggagaccgg ctcgcgtgca aggcgccttt
     2401 gaacgaagct gtggaggaga tccgtttggg tcgcgtcaag ccgtgcttct ggctgagaga
     2461 tgagccgttc cgaatcgcag cccagcgccg cagctacgag aaggcgcgca tcgcgcgccg
     2521 ccgattcgat gccactaccg cgcaggcggc gcgtggtctc cttgatcgcc gccgctgccg
     2581 taagcctgtg caactcgtta tcagatatgg ttgtgggcat tggcctcagc aagctgactg
     2641 gcctgatggt cggtgatgag tgcgtcgatc agctcgtcca gcgcactgcc atcgagaatg
     2701 gtgtcgagct tgtgcagggt caggttgatc cggtgatccg tcacgcggcc ctgcgggaaa
     2761 ttataggtgc ggatccgttg cgaccggtcg cctgagccga cttggcctcg gcgttgggcc
     2821 gcccgctcat ccgccgtacg ctggcgctcc cgctcatata tgcgggaccg cagcacctgc
     2881 atggcaagcc ggcggttctg atgttgagat ttttccgccg acacgacgac gatgcccgtg
     2941 ggcagatgcg taatgcgcac ggccgaatcc gtcttgttga cgtgctggcc gccggcaccg
     3001 cccgcgcgca tcgtatcgat acggatgtcc tctggcttga tctcgacatc tacctcttcg
     3061 ggctccggca aaacggccac cgtcgcggct gaagtgtgaa tacggccgct cgcctccgtc
     3121 tcaggtacgc gctgcacgcg atggacgccg gactcgaatt tcaaacgcgc gaagacacca
     3181 tcgccggaga tcgatgcgat cacctccttg atgccgccga ggtcgttctc tgacatggag
     3241 atgatctcgg ttttccagcc gtgcaggtcg gcgtagcgct ggtacatgcg aaacaaatcg
     3301 gcggcgaaca gcgccgcctc gtcgccgccc gtgccggccc gcacctcgag gatgacgctg
     3361 cggtcgtcgg cagcatcctt cggcagcagc agaatacgca gtttctgctc gagctcctcg
     3421 atcttcgggc gaagcgccgc cagctcctgc tcggcaaggg cagccatctc ccggtccgat
     3481 ccagcgtcct ccgcgattgc agtcagctcg gccagctcct tttcggcctt gcgcagctcc
     3541 tgaaccgtcg tgacgatcgg tttcaggtcg gagaactcct tggtgagctt ggcgtaagtc
     3601 gcctggttga caccctgact cagctccgcc tggatcacat cccagcgctg gatgagcttt
     3661 tcgagctttt cttgcggaac ggagttcatt gcgaacgttg tcgtctcccc ctgtgcccgg
     3721 ccgtgaccgc cggctgccgt ctgctcaaca atgagaacaa ttcgcgagcg ggagcaacga
     3781 ccgagcacgc actctcaatt cacatgctca cagattttga aaccgcggcc tggccgctct
     3841 gttgctctaa aggctcgctt tcaggccagc tcgaccccct cggctttggc gaagtcctgc
     3901 aaatacgagc gaatgctgcc ggacttcgtc tctagcaatt catccacgac gctagcaacc
     3961 ttggctgcat cgagcgacaa gatcatcgat ttcacgggac cgatggaggc cggtgccatt
     4021 gagatcgtcc ggtagcccaa cccaatcaag gccatggcat ccaaagtgcg ccctgccatt
     4081 tcgccgcaga gcgtcaccgg caccccgtgt ctggcgcccg cgcgcacgac ctgcttgagc
     4141 gcgcgcagtg ccgagaccga cagcgggtcg taccgatgcc cgacctgggc gttggagcgg
     4201 tccgccgcga acaggaactg catcaggtca ttgctgccca ccgacacgaa gtcgacctgc
     4261 ggcatcaacg catcgagttc atagagcagg ctcggtacct cgaccatggc gccaaggcgg
     4321 agcctctcgg ggcgcggcac gccgcgcttc gcgagatgat cgacttcacg agcgatcagg
     4381 gctttgaccg cctcgatctc ggacactgtc gagaccatcg gcaccatgat gctgagctcc
     4441 cgccccccag cagcccggat caaggcctgg atctgcgtgc ggaacaggcc ggggcggtcg
     4501 agggagatac gaaccgcccg ccagccgagc gccgggttct cctccttggg atggcggata
     4561 taggggagaa tcttgtctcc gccgatgtcg aggctgcgga acaccaccgg cttgccagag
     4621 gctccatcca gaatctgccg gtaggtctcc gtctgcttgt ccctccgcgg aaaggtagag
     4681 gagatcatga actgcagctc ggtgcggaac aggccgatgc cctccgctcc cgattcgtgc
     4741 agatgcggaa gatcgaccag caaccccgca ttgatgtgca gatggatgcg ggccccgtcc
     4801 ttcgtcacgg tcggcagctc gcgaagggct tggtaccggc gctgccgcct ggcccggaac
     4861 cggaccttgt ccgaataggc cgtaacgata tccgtcgagg gccggacgtg gatatctccc
     4921 ctctcggcgt cgacgatgat cgggtcgccg gtcgaaacca gctcgacgat tccccgcgcc
     4981 tgaccgacgg ccgccagccc gagggctcgt gcgacgatgg cgacgtgact ttggccgctg
     5041 ccctcctcga tgacgaggcc ccgcagcttc tcacggtcgt agtcgaggag ctcggcgggg
     5101 cccattgtgc gggcaaccag aatcgtgtcg gccggaagct ccaatccttc ccgggctgcg
     5161 gtcgtacggc cggcgagaat gcgcaacagc cggtcggaca aatcatcgag atcgcgcagg
     5221 cgctcccgcc agtaagggtc gttctggcgc agcatccgcg tgcgcgttgc gttctgcacc
     5281 cgctcgacgg ccgcctcggc ggtcaggcct tcccgcacgg ctgcttccat gcgccgcatc
     5341 cagccgcgat cgttggcaaa catgcggtag gcttcgagga cgtcgcggtg gtctccggcc
     5401 tgtgcgagcg cctccgcact cagcatctcg tcgaggctgg cacgcaggcg atggaccgcc
     5461 gactcgaggc gcttcaattc tgtcgccacg tcctcggcga gcagcttcgt taccaccact
     5521 cggggctcat gcagcacgac atgtccgagc gcgatgccct cggagatggg ctcgccgtgg
     5581 gccacgccgg caagcgagcg actgatctcg gcagcggcat tcacgccggc aacggcgccg
     5641 gagacgatgt gctcggcgac gaccatcgcc gtcgtcaaca gcacctcgac gtcctcgtcc
     5701 gagtactccc gccgcgtcct gttctggacg gcgagcacgc cgagcacgac cccgcccctc
     5761 aggatcggga ccgcgagcat cgagtgatag atctcctcgc ccgtttccgg ccgatacgag
     5821 aacgcggggt gaagctgcgc gtccggctca ttgatcggca ctgcgatctc ggcgcatcgg
     5881 ccgacaaggc cttcgccccg cttcatgcgg gtattgtgaa cggcttcctt cttgagaccc
     5941 tcggtcgcga aaagctcgag cgagccgtcc tggcgcttga ggtagatgga gcagacctcc
     6001 gccaccatca gtccggagat ctggcgcacg atcttgtcga gccgcgactg cccgtcgccc
     6061 ggctcggcca tgatctcgcg caggcgcctc agaataactc tgagcgcagg ctccgacggt
     6121 aatgtgcgcg gctcatcccc gcgtctggtc tgcatggcgc ttgccgttca gtagtccgtt
     6181 gcggacggcg gatagcagga ggcacgaggc ttccgatacc ccgtggacct catcggcacc
     6241 ttactcaagt cccgcgccag gcgggcgcgc tcaatccttg tcgagtccgt aaagggtatg
     6301 caaggtgcgt acggcgagtt cggcgtaggc cgcgtcgatc aagacgctga tcttgatctc
     6361 cgacgtggtg atggccagga tgttgatgcc cttttcggcc aacgccttga acatctgggc
     6421 cgccacgccg gcatggcttc gcatgccgac tccgatcacc gaaaccttca ccacgtccga
     6481 cgagctgtct atttcggagt acccgatcgt ctcctgacat ttacgcagga cctccagcgt
     6541 cttgggcagc tccgaggcgg cgacggtgaa ggtcatgtcc gtatgctttc cgtcggagga
     6601 caggttctgc acgatcatgt cgacgttgac gctggcgtcc gccaggggcc cgaaaatgct
     6661 ggcagcgaca cccggtttat cggccactcg ccggatcgtg accttcgcct cgtcctttgc
     6721 gtaggcgata ccgctgacaa cctggctctc cacgatttct tcctcgtcgc aaacaatcgt
     6781 gccgatgttc tgccattccg caccgccgcg gaccggctcg atcgtctcgg catccgcaaa
     6841 gctggacagc acgcgcgtgc gcatccggtg gaccatcgcc agctcgacgg accgcgtctg
     6901 cagcaccttc gagcccagcg aggccatctc cagcatctcc tcgtaggaga tccgcggcag
     6961 acggcgcgcc gtcgggacga tgcgcgggtc ggtggtgtag accccgtcca cgtccgtgta
     7021 gatgtcgcag acatcggcgt tgagcgcgat cgccagcgcc acggcgctgc agtccgaccc
     7081 accgcggccc agcgtagaga tgcggttgcg ctgtggctcg agcccctgaa aacccgttac
     7141 cacggcaacc tcgccttgcg ccagccgctg ccggatttca tcggcctgga tctcctggat
     7201 tcgtgctgcg ccatgggccg cgtccgtgag gatcgggatc tgccagccct gccaggagcg
     7261 ggccggaacg ccaatcgact gcagcgcaat ggcaaggagc cctgcggtca cctgctcacc
     7321 ggaggcgacc acggcatcgt actcgcgcgc gtcgtggatc agcgacatct cccgcaccca
     7381 ggcgacgagc tggttggtgt gtccagccat tgccgagacg acgacggccg cctggttttt
     7441 ggcatcgacc tcgcgcttga cgtgacgggc gacgttgcgg atgcggttga tatctgcaac
     7501 ggacgtgccg ccgaatttca tcactacgac tgccatgcgt ctacccgttg cgccaatcac
     7561 ctcatgctcg gtgccgcggg gacgcggtac agagggcaag gcaaccggtg cttctcctcg
     7621 ttgtcagcgg aatggcccga tcgggccgta ctcatagcga taacgcacca gagctgcaac
     7681 caatgcggtg tatccttgac atgagtcaca agaatgacgc gagatcccgg tcatgacatc
     7741 cgcgcatcca agccaagcca ctctggatcc tgaggaaatc gatcgttttg cccgtttggc
     7801 gggcaagtgg tgggatcctg agggggagtt ccggccgttg cacaagcttg ggccggcgcg
     7861 cctttcgttc atcaagtcgc atctcgtccg ccgcttcggc ctgccggaac gggggcttaa
     7921 gcctctcgcg ggcctgacga tcctcgatat cggatgcggc ggcgggctga tctccgagcc
     7981 tctggcccgc cttggcggga ccgtgacggg aatcgatcct gcaacggaga acatcgcgac
     8041 ggcgatccag catgccgaac cgcaggggct cgtcatcgac taccggaccg cccgtgccga
     8101 ggatctggtt gaggagagga cccagttcga cgcggtcgtc tgcttggagg tcgtcgagca
     8161 cgtaccggat ccgcaggcct tcattcggac ctgtgccgct ctggtgcgtc ctggcggact
     8221 tttcatcacg tcgaccataa atcggacgct caaggccttc gccctcgcga tcgtcggcgc
     8281 ggagtacatc ctgcgctggc tccccgtcgg cacccaccag tgggatcggt tcgtaacacc
     8341 ggaagagctg gcgcgctatt gccaagcggc cgggctgcag cagcccgtgt tcgagggact
     8401 gatctacaat ccactcacgg ggcgttggtc cttgggccag gatacggacg tcaactacat
     8461 ggctgctgct gacaagccgg gctcgacgag ctgaggccgc ttggaaatcg cctgcatcag
     8521 ctttgggcga gctgaaggat ccgctgcacc atcgcactct ccgggcgcgt caactcgtca
     8581 accacggtga agtggtttgc attcggcacg accacgcact cggccttcac gccggccgct
     8641 gaccacgccg ccgtgatgtc gaggctctgg cgcaggaact cctggctttc cgcaccaccg
     8701 acagcagcca cgaaggtgcg gtcgcgaggc ggtggcggcc agagaatcgg gctcgcctcc
     8761 cgcgctgcct ccggcgtaag ccgcagcgcc tcattgaggc tcgtcgggat cagcggcgtg
     8821 aggtcgaaga cgccgctgat cgcataacct gcgcggacga gatcagccgg cgcatcgtgc
     8881 cgcgaccagt ccgtcgccat catggctgcc gccagatgcc cgccggcgga atggccgacg
     8941 atgacggggc gcttggcagt gcgccgccaa agcgcgacga gaaactggcg catctcatcc
     9001 acgatttgcg caatggtggc ctcggggcac agagtatagg agggaagtgc caccacagcg
     9061 cccctgccga ccagggcttc ggccacgaag ctgtagtcga cccggtctcc acgctgccaa
     9121 tagccgccgt ggatgtaggc gaccaaaggt ccatccgcgg tgccttccgg agctgcgaag
     9181 agatcaaacc ggttgcgagg ccccgatccg taggagaggt cgagctcgtg agccagcgtc
     9241 gcccgcaccc gcgcggagtg agcagcccat ctcgccatga tctccgtgtg ctccgggacc
     9301 cggcggcggt tgttgtattc ggcctcgtag tcgatctcag tcatgatttg ccccctcagg
     9361 cagcttgcgc gccctgccgc gcgtcttccc ggatcatgtc tgacgctttt tcggcaatca
     9421 tgatggtcgg cgcgttggta ttgcccgatg tcatggtcgg catgatcgag gcatcgacaa
     9481 cccgaagccc cgcgatgccg tgaacacgca ggcgctcatc gacgaccgcc atagggtctc
     9541 ggcccatctt gcaggttccg cacgggtgaa agatcgtcgt gcccttcgcc ttggcgaagg
     9601 ccatcagatc ttcatcgctc tcgacctccg gaccgggaag gaactcgcgc ttgacgtact
     9661 gcttgaacgc gggctgctcg gcgatccgcc gaagcatgcg gattccggcg agcaacacgt
     9721 cgcaatcggt ctgggacgcc aggtagttcg caacgatgct gggcggcgcc gcgggatcgg
     9781 gtgacgtgat cgagatcacg ccgcggcttt ccggcctgag ctggcaaacc gaggcggtta
     9841 cgcccgaaaa tgggtggagg ccctggcccg gcccttcagc agacagcggc atgaaatgga
     9901 cctgaacatc gggaacgtcg agctccgggc gcgagcgcgc gaaaacgccg accacgccgg
     9961 caccgatggt gagaatgccg ctccgcgaga aagcatattc caggccagcc cgaacctgca
    10021 tcagacggct gtgccagaca tcattgaggc tagcgggcaa ggagcactcg tagacgaagc
    10081 gggcctgaaa atggtcctgc agattttcgc cgacgccggg aagctcatgc aggacttcga
    10141 caccggcatt cttcagtacg gctcccggcc cgatgcctga gacctgcagc agttgaggac
    10201 tgccgatcgc gcccgctgcg agcaacacct cccgggcggc cgccactgtc cgcgtttgcc
    10261 cgtccctctt gtaaacgacg ccggtcgccc gccggccatc aagggaaatg cgcttcacct
    10321 cggcgttggt gatgatggcg aggtttttcc gcgagcgcac ggggttgaga tagcccaccg
    10381 ccgtcgagca gcgccggcct ttccacgtcg tgagctgata atagccgacg ccttcctggc
    10441 tggctccgtt gaagtcggtc gtccgcggta tgccggcggc gacgcaggcc gcgatgtagg
    10501 cctcgcacaa cggattcttc atgcgcacat cggagacgcc gagcggtccg ccggcgccgt
    10561 gatactcatc tgcgccccgc tcctgattct cggcacgctt gaagtagggc agcacgtccc
    10621 gatacgacca gccggcgttt ccgagctggc gccaggtgtc gaaatcctgc gcctgaccgc
    10681 gaacatagag cagcccgttc accgcgctgg tgccgccgag aacgcgcccg cgcggccatg
    10741 gaacgatgcg gccgttgagg taaggctccg gcccggcgcc gaactgccag gtgagcttcg
    10801 ggttgaacat ggtgcggaaa tagcccgcgg ggacgtgcag ccacggattg agatcccgtc
    10861 cgccggcttc aagcagagcc acccgcacgc ctgggtcggc gctcagccgg ttggcaagca
    10921 cgcaaccggc ggagcctgcc ccaatgatta cgtagtcgaa cgtttcggcg cccccatcag
    10981 actccatcgt aacctccgcg cacctgatgc ccccgatcaa tggtcagctt caggcttatt
    11041 acgactcctt cggccggagg gcacgataga tttcgatgac ctggctgtcg tcccggttgc
    11101 caaggccgag cccggatgcc gcaacgaaca gctgatgcgc ggcggcggcc agtgggagtc
    11161 ccatctttac ggaccggcct gcctccagaa cgatgccgag gtccttgacg aagatgtcga
    11221 gggcgctcgt cacctggcca tcatcggcca ccatccgggg tccgcggttc tgcagcatcc
    11281 agctcgaggc cgccgagccc gacaggattt cctgcgcgac cttcggatcg acacctgcct
    11341 gctcggcgag agccagtcct tcggctgcgg tggcgatgtg cacgccgcag aggagctggt
    11401 tgatggtctt catcacggcg ccttgcccgg gctcctcgcc gagatgatag atgttcgtgc
    11461 ccatcgcatc gaggatgggc ttggagcagg tgaaggctgg ctgcgctgca ccgaccatga
    11521 tggtgagcgt tccagccgtc gcgccggcaa cgccgcccga cacaggtgca tccacgaagc
    11581 tccggccggt gtccgccacg cgctttgcca tagcttcaac gcgacccggt gggcaggtcg
    11641 ccatcaggat caccgtcgcg tcggaagcca ggacatcaag cgtgccctgc tcaaagagca
    11701 cgtgctccgc ctgatccgca ttgaccacca tcagtataag gtgatccgcg ccatcggccg
    11761 cctcgcgcgc actcttggcg ccgtggccac cagcctcgac gaggttcgca accgcttcgg
    11821 cgcgaatatc gtatcccacc acccggaatt gtttccgcac gaggttctgg gccatcggca
    11881 gccccatggc tccgagcccg atgaaagcaa tcgtctccgg cattttcgcc cacttgctgg
    11941 ttggcattgc accattccgg ctcccgtggc agaggccgga tcaataccag gaacaatgca
    12001 ccgggcacgg cggctgcgct accctttgat catgggtcaa atcgcggctt ctgtctcgac
    12061 atcgaagaag tgcaggcggt cgggattgac ggcgagccgc aacttctcgt cgactttcgt
    12121 cctcaagctc gggtcgacgg ctgcgaccat ggtggcgttg ccaacggaga catcgagcag
    12181 gatctccgag cccagctgct cgacgacctc gacgacggca tcgaagcagc atcccgctgg
    12241 gtcggccgag ttggcgacgc gcaaatcctc agggcgcatc ccaagggtca cctcgcgccc
    12301 gacgtgactg cgcagtcttg cggcgctccc gtcaggcact ctgagccgca taccgctatt
    12361 ctgcagccag agctcgccgg cttcatcctg caccgtcaca ttacagaagt tcatcgcggg
    12421 tgagccgatg aagccggcga cgaacttgtt ggcgggtgaa ttgtaaagct ccagtggctc
    12481 accaacctgc tgcacccatc catcccgcat gaccacgacg cggtctccca aagtcatggc
    12541 ctcgacctgg tcgtgggtga cgtagatcgc cgtcgtgccg aggcgatcgt gaagcttctt
    12601 gagctcgaca cgcatttgca cgcgcagctt ggcgtcgagg ttcgacagcg gctcatcaaa
    12661 aagaaacacc tgcggattgc ggacgatcgc gcggccgagc gcaacacgct ggcgctggcc
    12721 gccggaaagc tggcgaggct tgcgatcgag gagttgctca atccccagga tatctgcggc
    12781 ttgcttcacg cgctgtctga tttcggcctt gtcgaatttc cgcattttca ggccgaaggc
    12841 catgttgtcg aaaacgctca tgtgtggata aagcgcgtaa ttttgaaaga ccattgcaat
    12901 gtcgcgatcc atcggcggca agtcgttaac gatctgctca ccgatcagga tgtttccgga
    12961 gctggtcgcc tcgaggccgg ccaccatcct cagcgtcgtc gtctttccgc agcccgacgg
    13021 gccgacgaag accatgaact ccttgtcgtg gatcgtgaga ttgacgtctt tgaccgcgtg
    13081 cacgctgtca tagaacttgt tgatctgctt caggacgacc tgcgccattt tccgattacc
    13141 tttacgcgac ccgctttcag cccttcactg agccggtcag acccgacacg taatgttcga
    13201 cgaagaatga gtaaacgagc gcgaccggta tcgagccgag cagcgcgccc gccatcaatg
    13261 gaccccagaa gaacacgtca ccacggatga gctccgaaac gacaccgacg gggaccgtct
    13321 tctgctccgg cgaggacaaa aagacgagcg cgtaaatgaa ttcgttccag gagagcgtga
    13381 acgcgaaaat tccggcggac aggatgcccg ggacggcgac tggcacgacg atgtagagca
    13441 tggcctgcca gcgcgaggcg ccatcgatgc gcgcacattc ctccagttcc ttcgggatgg
    13501 ctttgaagta gcccatcatc agccaggtgc aaaagggaat gaggaatgtg ggataggtca
    13561 ggatgagcga ccagggtgtg tcgcccagcc tgtaatttcg gatgatttcc gacagcggaa
    13621 tgaagagaag cgtctgtggc accagatagg tgatgaaaat gccggtgccg aggaatccgg
    13681 caaagcgaaa gttcaaacgg gcaagggcat agcccgcgaa aactccgcaa atgagcgaaa
    13741 tgaccgtgga tacgatggcg atgaacatcg tgttccacag ccatttcagg aacatggttt
    13801 cttgcagcag gtaccggacg tgagcgagcg tcggcttcca tgtccagaac gggttgtaat
    13861 ttacggcgtt ccaggggcga tagagctcgc catccggcct gaacgacgtg atcaacatcc
    13921 agtagaacgg gaacagaagg accagaatga acaggagcaa ggggagtcgg aagtagaccc
    13981 agcgtctcca aacggcacct tcgatcatga cttcagcgga tctccaccct gcggatgtat
    14041 aggagctgga caacgacgat caggaacagg atcgggaaca ttgcgagcga gaccgccgcg
    14101 ccttggctca gcaatcccgt tccgacgccg agttgatagg cgtaagtggc aaagaggtgc
    14161 gtcgcgttgg cgggtccacc gccggtcatc acgtagacga tctggaagtc ggcaaaagtc
    14221 tggatgacag agaacagcac caccaccatg gtgaccggca gcagcagggg ccaggtgatg
    14281 taccagaacc gctgcaacgg ttttgcccca tcgatcgcag ccgcctcatt caggtccggg
    14341 ctgatggttt gcagccccgc cagcaggctg attgcgaaga aaggaacgcc ccgccagata
    14401 ttgacgatga tgatggagat cattgccaaa tcgccatcac caagccagtt gatccgccgg
    14461 tcgatgatgg agaggtgata catcagccag ttcaaaacgc tgaaggtggg atcgaacatc
    14521 catttccagg cgaacgtgga aaggacagtc ggaatgatga acggcaacag gatgaacgcg
    14581 cggacgtagg ccttgccctt gaagctgcga ttgagcagca gcgccagcca caagcccaac
    14641 cccagtttga atacagtggt caccgccgtg taccagacgg tattccagac cacccggtga
    14701 aagatcgggt cgccggccag ccgctggaag ttctccaatc cgacgaactg gcccggaacg
    14761 ccgacccgag cgttcgtgac ggcgagaatg atcccccgaa agaaaggata agcaatgaag
    14821 agccccagga ggatggccgt cggcagcagc aaaacgagag ccagccagcg ctcatcctcc
    14881 aaaagccgcc ggcgtggtgg cgtcttggcg gccaccgtgt cggcagtgag tatcgccata
    14941 tttcgatctc ttacgaatac gtgctgacta tgatcgcctc accttcgaag atggggcgat
    15001 ccgagcccca atgcggggcc gcgcgcggca cccgtcaatg cagcatcacg actcgtagat
    15061 tttcttcagc tcggcgtggg tctgcttcac gatgtcttcc gcgcttgcgc ctttgaccgc
    15121 ctcggcgaac atgttgacga tgagatattt cgaaagcacc tcagccgctt ttgcattggg
    15181 cggacccttg tagcccgggg tctggcccag cttgcccgcg atggcgaaag gcttcatcac
    15241 cgggttctcg tcccacatct tgtgggtctc ccagctcgcg gtgcccggag tgtaatagcc
    15301 tttggccgtc gtgaaccact gctcatagtt ctgcttggcg tgcacgaact tgagcagctc
    15361 tttggccgcc gactggttct tggagtagga cggcatcacg tgcgaggtga acgtgtggaa
    15421 cccgaactgc cccttgggac ccgccggcag tggcgcatgc cggatgtcat ccttcatcgg
    15481 cttgccatcg gctgtctggt actgttcggc tttgcgcact gcttcgacgt aaatcgatcc
    15541 gccgttcaag gttgagcaga tcgtgccgga taggaaggcg cggttgttgt tggagtcatc
    15601 ccaagccagg ccgccttcat cgtaggcatc cttccaggct gcggtcaggt atttcaccgc
    15661 ttcgaccgtc tccttcgaat cgagagcgac ctttccgctt tcgtcgatct cgacaccgcc
    15721 ataggcccac atcagcggat aagcaaacgt tggaggatcg ccgaacgatt ggccgagcgc
    15781 ctggccgatc ggcagccctt tggctttcag ctttttcccg gcatcccggt attcgtccca
    15841 ggtcttggga aattcgttgt acccgacttc agcgaaggcc gacttgcgat aggcattcat
    15901 cgcgccaatg atggccattg ggatgcccat ccacttgcca tcggcgctgc agttgccctt
    15961 cgagatatcg taaaagccgc cttgctcctt cccgatcacg tccgcgacgt cattcagatc
    16021 gacaaggctc gccttgtaaa gatgcggatg gttgttgaag agcatgatga tgtcagggcc
    16081 tgagccggct tgaatggcgg acgtaatgcg cggctgcagg tcgttgccgt tcacggtctc
    16141 gaagttcacc ttgatgccaa gggctttttc tgcctccggc agcagcttct gccgcaggag
    16201 ctgatcgcag gccggcacga agtcattcca cctgagccag tgcacagtgg tggtctgcgc
    16261 ataggccggt gcctttccgg aagccaaaat ggcagccatt ccgcccgcct tggcagtgat
    16321 tgcgccggcg ccggacaatt tcaaaaaatc gcgacgatga actcgcgcca ttccatttcc
    16381 tccctggatt atgctcgcac gcactgaaag tgcgaagttt catgggtcgg gttagcctcc
    16441 cgccgcacaa tgtatacttc aatctgcgcc ccaatgacac agtaaatatc gccagataac
    16501 ttaggagaag aaatattagt cagcagatta tatgccaatc atctttgaga acgatttgac
    16561 tgggtttgca tattcggaat gtctgacttc caagctggcc ggttgtctga aaacgaaaag
    16621 ctggttatcg acggccccgt cagaaccgag ccgggatcgc agaaagaatg tacgagagtg
    16681 cagtcgccac cctctcacga gaagccgtag ttttccgaac gccgtgcgga agcggggatc
    16741 tcgtctggcg gagatgggga acgggtgcgc ccgtcgtcct ctgccatggc ggatccggct
    16801 cctggaccca ctggatcaga acaatcccgg cgctgtcgag gcgatacgag ctgtgggtgc
    16861 cagacctccc cggcctcggc gatagcgcca tgccgccgaa gccgtggacg cccgcgacat
    16921 cggcgagcat tctgaccgcg ggattgcgac agctcgtgtc gccggacagg aaggtccacc
    16981 tcgtcggctt ttcattcggc gctcacgtgg cgacgctcgc cgcagtggag ctgggggact
    17041 ggcttgcaga cttcacgatc atcggcagcg ccgccctcgg gctgccatgg cattcgctcg
    17101 aattgcccaa ggagcgttcg acgatgtcgg cggaggagcg ccgtgctgtc caccggagga
    17161 cgctggaaat tctgatgttc gccgatccgc gccggatcga tgaggaagcc atcgcgattc
    17221 agatggaaaa cattgccaag gcccggtttc gctcgcgcga gttcgctgcc accgacgaaa
    17281 tccgcacgac gctggcgcgt gtgacggcgc cgctgcgggc catctggggc gagcgcgatg
    17341 cgattgcacg cccgagcgtt gaagcatgct tcgacgttct ccgcctctac cacccggagt
    17401 tggtgacccg cacgattccc ggagcgggtc actgggtgat gtacgaggcg ccggacgcct
    17461 tcaatgccgc gctgatggag ctgctcgagc ttccgccaaa tcgagcctga gccgggatgg
    17521 cgctattgct tcgcatccca gcccggtgcc ggttccgccg gataagatga aagccccgca
    17581 gcgcgaatgg cctcgatgca gcaggcctcg tccttgtcgg atgcgtcccc gctcatacca
    17641 accgcgccga tcacgcgctg atcggcatcg aggatcagca cgccgcctgg caccggaatg
    17701 aacttgcctt gcgacaaagc cgacagcgcg gcctggaatg tcggccgctc ggcgagacga
    17761 tcccggctgc cgcgactgga cgtccccatt gcgagcgccg tccaggcttt tgcctgggca
    17821 atttcgcagc ggaggaatgc gaggccatcc tggcgcttga acgcgacgag atgaccaccg
    17881 gcatcgagca cagcggcagc catgggcgct gtcccgatcc ggctggctgc ggtgagcgca
    17941 ccatcgacga tccgctcggc aagctcgagc gagatggaag ccggcaggag atgcagctca
    18001 tggcttacca cgttatcctc cctcaaacga cttcgacttc caggttgccc acgccatcca
    18061 cgtgtcccag caggcggtcg cctctcttaa ccgccccgac gccggccggc gtgccggtga
    18121 gaatgaggtc tccggccttc agttcccaca ggcccgaaag gtactcgatc tggctcggaa
    18181 tatcccagat catctgggaa agatcgcccg actgccgccg ttcgccattg acgtcgagcc
    18241 agatggcccc cgtggtcgga tggccgattt tttcagcagg cacaatggcg gagcacggtg
    18301 ccgacttttc gaacgattta ccgatttccc aggggcggcc cattttcttc gccatgccct
    18361 gaagatcgcg ccgtgtcatg tccagagcca ccgcatagcc atagacatgg ctcatggcct
    18421 gcgcgcgcgg gatgttcacg ccgcctgatt tgagcgccac caccagctcg atctcccaat
    18481 gaacgtcctg cgaggcggac gggtagggaa acctgccgtc ggtcaccagc gtatcgggcg
    18541 ccttcatgaa gaagaacggc ggctccttgt ctggatcgaa ccccatctcg cgcgtgtgct
    18601 cggcgtagtt gcggcccaca cagtagatgc ggtggaccgg gaaaagcttg tccgagccaa
    18661 tgatgggcag cgcaggcacg ggcggcgggt cgaacaggaa aggcatcggc gtttgtcctc
    18721 aggcgtatgg cttgcttgta gcccggagca ttagcagatc gtcctgcccg gggcgagaca
    18781 tcctgcaccg cgcaactctg gcccgcgaat ggctttccgg catggatccc aggccgctcc
    18841 gctcccgttt gatgcctgcc ggcagggtgg agtgcctctg ccctgcgccg gtcaatccag
    18901 gcacagatca aaaggagagg acgcatgttt ctcagaattg atcggcttca ggtcgagatg
    18961 ccgctgccaa aagaccctga tccggctgca gcggcggcgg tgaacgagct gatgggcggg
    19021 cggttcggcg agatgaccac gctcaatacg tacatgtatc agagcttcaa tttccggatg
    19081 cgggacaagc tgaggccgtt ctatgccctg attgccaaca tcgccacgga agagctcgga
    19141 catatcgagc tggtcggcgc tgccgtgaat ggattgcttt acggagcgga tggaaaaggc
    19201 agcgttgctg cgggtgatgc tccgttcgag gatatgttta agcaggcggg tgcaggcgcg
    19261 aactatcaca tcaacggccc gcacggcatt ctggcggcca actccctcgg ccagccctgg
    19321 cgcggcgact atgtgttcaa ctccggcaat ctcgtgctgg atctgctaca caacttcttc
    19381 ctggagtgcg gtgcgcgcac ggtgaagctg cgcgtttacg aaatgaccaa gaaccccgct
    19441 gcgcgcatga tgctgggcta tcttttcacc cgcggcggcg tgcatgccca tgcctatgct
    19501 ctcgccctcg aaaagctcac cggcgtcgag gtgaggaaga tgctgccgat cccgcgcatc
    19561 cttgatgcag aaattccgga atgcgacaaa ttcatcaagg agggcagtca tcggaagctg
    19621 taccgcttca gccccgatga ttacaaggaa atcgccgcca tctggaatgg cccaggcctc
    19681 gacgcatcgg aaatcctcga ggtcgtcgat ggtccgccgc agggtggtcc gcagtaccag
    19741 atggacggtg tgcccgaagc gttcattccg gaatatcatc cggaggaaat cgtcgacatc
    19801 gccaagaagc tctacaccgc ggcgggtggc tcacagccct gatgaggctc cgtcggacat
    19861 agtccggtcg cggtgcgtta tacaagagca ggcgcagcgg gtcgggtcga aggcggcctg
    19921 ctgcgcatgc gaacatcaag agagctactt atttatgtcc tcgacctgga cctgcacgtc
    19981 tcgctccgac ccatttcgca gcacggtcaa ggtaacggta ttgccgatcc cggccttctc
    20041 gagctccctg gtcaagtcgg agagctcatt gatgcgcttg ccattcacac caatgatgat
    20101 gtcgccaaga cgcccgagct cgtccatgcc cttgaggccg gcgcgatcgg ctgccgaacc
    20161 cggcgtcact ccctgaatca cgacgccagg agcgcccagg cgggcagtca gctcgaccgg
    20221 gaagacgcga atgccgatgc ccggcagcgg cgcgcgtcct gtccggatca gctcaggcac
    20281 gattcgattg acggtgtcga ccgggatcgc gaagcctacg cccgcaaacg ttccggacgg
    20341 tgcgagaatg gccgtgttca cgccgatgag gcggccggcg ctgtcgacca aggggccacc
    20401 ggagttgccg ggattgatgg cggcatccgt ctgaatcacg ccggcgatct cgcggcccga
    20461 ttcggtgggg agcgtccggt cgagcgcgct gatgatgccg ctcgtcaaag tccgggacag
    20521 gccgaacggg tttccgatcg caaataccat ctgcccgacg accaggtccg cggaggtgcc
    20581 cactttgatc ggctgcaagt ctggcggcgg gtcgatcaac tggagcacgg caaggtcgac
    20641 ccatggggca gttccgacga cgcgcgcggg atgcaccttc ccctgattga tgacgacccc
    20701 gacctcatcg gcgccttcga cgacgtgggt gttggtgacg atgtgcccgg cgcggtccca
    20761 tacgaagccg gatccggccc ctgctctctc cagggctctc cctccgggcc gctgcgtcgc
    20821 ttggtgcgta aagatgtagg ccaccgaagg cgcggtttcc gcgtacaccc tggtcgcgat
    20881 ctgttcaccg ggcgtgagat cgggacgcgg cgtcgtttcc cttggctctg tggcggcgaa
    20941 gacaaatacg cgcaaatacc gttcgccgac gaacagggtc gcaagtatga gcgccaccac
    21001 gatggcaatg cgccagaagt gctctctcga cgtgacttct gccatgtggg ttcgccccta
    21061 ctgtgataac cgagaaattc tcacggctct ggcgaacttc gcaatcggag caccagcctg
    21121 cagtttcgct gtaaacgcgg gttgcagtcg ttcgttgctg cgatcaggcc cttacggccg
    21181 accggcggct tcagcttcag ctttgctttt ttagccttgc ccgctccact tggacgagat
    21241 cgcacagcac gtcgaccatc gctgagaagt cctggttgcc atagccgcgg gcgcgggcac
    21301 tggagaaggc ctctcgcgcc gccgctgcga tcggaacggg tagtcgcgca gcgtttgccg
    21361 ctccaacgat cagcgacaga tccttgtgcg cgagatcgat ggtgaagccg ggagtggtgt
    21421 cgccggccag gaccttgttc ggccagttga tcttcagctg gccgttggtc gccgtggttc
    21481 cgtgaatgac ttccagcgtc ttttcgagat cgagaccgaa cctctgagaa agggccaggg
    21541 cctccgcgtt gagctggcag gagatgatgg cgaggtagtt gttcacgagc ttggtccggg
    21601 tgccggtgcc cccgggaccg caatggtgga tggtggtacc catggcttcc agcagcggct
    21661 tcactcgctc gaagtcctca ggctccgcgc cgaccatgaa caggctttcg ccgcgttcgg
    21721 catgccaggc gaggcgaccg atcggcgcgt cgacggccct gaagccgcgc tgtttcgcct
    21781 cggcgtggag gcggtccgtg gtctccggct cgaccgtcga gaggtccatc accagcgtac
    21841 cgggctttgc atgatcgaag atgccgcccg gcgccatcac gatctcggtc acttccttgc
    21901 ccgcaggcaa tacggtgatg atgatcgagc actggcgggc gacgtcggcg atgtccttgc
    21961 ccgcggttgc gccgagcgac acgagctcct tcaccggctc gggtcgcaga tcatgcacaa
    22021 cgagcttgaa gcccttcttc acgcagttgg ccgccatgcc gcggcccatc gcgcccaacc
    22081 cgatgaagcc gatcgtttcg tctcgcgctg acattcctga ggttcccccg atcaatcgat
    22141 tgccgcacga tttctcgcag gatcatggca atcggacaag gtcctagccg gccttctggc
    22201 caacttgtcg tccaacatca gacggcttcg aggaagcgtt cggcgttggc gaggtcgacg
    22261 gagacgagct ggctcacgcc cttttcagcc atggtgacac cgaacaggcg gttcatgcgc
    22321 gccatggtca tcggatggtg cgtgatgacg aggaatcgag tctcggtctc ctcggccatc
    22381 cgctccataa gcgtgcagaa ccgatcgacg ttggcatcgt cgagcggcgc gtcgacctca
    22441 tcgagcacgc agatgggcga cgggttggtg agaaacaccg caaagatcag cgacagtgcc
    22501 gtgagagtct gctcgccgcc cgagagcagg gacagtgtcg ccggcttctt gccgggtggc
    22561 ttggcgatga tctcgagccc gccttccagc ggatcctcgc tttcgatcat ctcgagccgc
    22621 gcttcaccac cgccaaaaag agtcgtgaag agccgctgga aatgaccgtt gacgagatcg
    22681 aaggcattct gcagccgctt gcgggcctcg cggttgagct gcccgatgcc gccgcgcagg
    22741 cgtgcgatgg cagcctccac gtcggcccgt tccgcgtcga gcttggagta atgctcctgc
    22801 atcgactgca gctcttcctc ggcctgcagg ttgacgccgc caaggcgctc gcggtcggcc
    22861 ttgagacgtg acaagacgcg atcgacgtcg gccagcttgg gcaagggggc gcccggcttg
    22921 atcccagcga tatcgagaca tccctccggt gcgacgccga aggtgtcccg aattcgggcg
    22981 acttcctcgc tgcgccgctg gcggacggct tcgagcctcg cctcgacgcg ggcgcgggac
    23041 tctcgctccg tgctgaccgt agcttgggcc gcccgaagcg cctgggcggc ctcccgctgc
    23101 gccgcctcgg ctgcggcgag gcgatctgcg gccgctttgc gctcccgctc tgcctcagcc
    23161 agcgtattga gcagtttctg acgcttttcc tcgatcagcg gtggaagctg ggcgagctca
    23221 tcgagttcgg cctttgcttc cgcgaggcgg gcctgcagtg tcccgatctg tcgctcagcg
    23281 cttgccgccc gctgcgccca tcgctcacgc tctgccgccg ctttcgtctt gcgctctgca
    23341 cgcatctgcc gctcgcgctc gagtgcggcg agagccgtgc ggagctgggc gaccctttcg
    23401 cgcgcgatcc tggcttgctg ctgagccgag gccatgagcg gttcatagtc ctcgcccgag
    23461 ctgaggtcgc tgagttcgtt ttcgacctct acaagccgcg cgcgggcctc cttcacgctg
    23521 gcttcggcac gcgcttttgc ctccctgacg gcggcaatcc ggccctctgc ctcgcgagcg
    23581 gcgcgttcga gggcgttgag ctgatcgcgc aattttacca gccgcgactg ggtctcgcgc
    23641 catgcctgcc gcagccggcg ttcatcctcc tctgccttac gctggcgttc ggcagccgcg
    23701 acggcctccg atgcggccgc ggccgcggcc tcgcgcgcag cggcttcctt gtgtctcagc
    23761 tctaccagcc ggtttcgctc ggcaaggcgt tgagccgccg gcgtcggtgc atcggcagcg
    23821 gcgacgaacc cgtcccagcg ccacaggtcg ccttcccggg agacgagccg ctggccaggc
    23881 ttcaacgccg gctgcaatcg tgccccgagc tcgcgcggaa cgatgccgat ctgcagcaag
    23941 cgccgggtca gctcgggcgg gccatcgacc gcctgggcga gcggctccgc gccttcaggc
    24001 agcggcgcat catcgggcgc aggctctatc aggctccaat gcgcggcggc ctcgggtgcg
    24061 attggcgcat cgaggtcttc gcccaatgcc gcgccgaggg cctgttcgta gccaggggcc
    24121 acctccagca ggtcgacaac tgccggatag ggagtgctct gcgccggcgt cagcagcttg
    24181 gaaagggtcg ccagctccgt agtgagctga cgcgcgacga gctccgcctg ccggctcgct
    24241 tcgcgggtcc gccttgcttt ctctgccgcg gcggcgacgc gctcctcggc ggcgactgca
    24301 ttcgtctcga gctctgccgt ctccagtgtc agctctcgga ccagctcctg cagctccgcc
    24361 agccgggtcg tatcgggcgc tttcgctgcg atgtcgcgag cctgcgctgc gagcgactcc
    24421 agctgacgtt ccagcttggc aacctcatcg cgtcgttccg tcagcgcggc ggtgaggctc
    24481 tgcttcctgg ctcgtgattc gtaggtgcga gcggtagcgt cctgcagtgt agcctcgatg
    24541 cgctcgagct cgctttgagc gctggccaga tggtccctgg ctgcctcctc ctgctcgaca
    24601 gcttgctcga ttgcaccttt gagcgcgtgc tcctcctgct cgagctgact gatgaggtgc
    24661 cgcgcttcgc ggatcagacc atcctcgcgc tcgaggtcgg aggcaagctg cgcgatgcgg
    24721 cctttgagct ccagctcgcg ctccttcatg cgctccgctt cgcgctcgaa cgtttcctgc
    24781 tgcaccttga tgcgggacag cacggcagcg cgggtggcct cctcatcgcg cagcggctgc
    24841 aaggattggg cgagttccgc ctcgcggcgg acgagctcgg attcttcctt taccgcgacc
    24901 ccgagacggg ccatcgcctc cgtgagcgcc gcctcctcgg cttcgacctg agcctgcgcc
    24961 tccacccatt gcagatagag tagcattgcc tcggctctgc ggatctcgga cgacagctcc
    25021 ttgtatcgct tcacctgacg cgtttgccgc ttcaggctct ccatctgcgc actgacctgg
    25081 ccgaggatat cggcgaggcg tgccagattg gcctcggcgg ccttcaggcg aagctcagct
    25141 tcgtgtctgc ggctgtgcag gcctgcaatg ccggcagcgt cttcgagaat tcggcgccgt
    25201 tgctccggct tggcgttgac gatctctccg atctgtccct gccggacgag ggacggcgat
    25261 cgcgcacccg ttgcggcatc ctcgaacagg atcttcacgt cgcgcgcccg cacctcccgc
    25321 ccgttgatgc ggtaagtcga gccggcatct cgctcgatcc tacgggtgat ctcgatggtg
    25381 tcgctgtcgt tgaattcggc gggcgccttg cgctggcggt tgtcgaggaa aatcgtcacc
    25441 tcggccgagt tgcgcgcggg ccgcgaggtc gtgccggcga agatcacgtc ctccatggcc
    25501 gaagcacgca tggacttgtg cgaggtttcg cccatgaccc agcgcagcgc ttcgagcagg
    25561 ttcgacttgc cacagccgtt cgggccgacg acccccgtca ggcccttctc gatgagaagg
    25621 tccgtgggtt cgacgaacga cttgaagccg agcagccgaa gccgctgaat gtgcatctct
    25681 cgccttgtcc tcgcacagcc gccaaggctt cggtgagggt tccgcgaccg gtgcccctat
    25741 cgggtggcgg tgaccttacc cggtccggaa ctgttggcca gcatcggctc gaccatctcc
    25801 cgtatctctt ccatcccgat caccgtcttc acgagacggt tgttgatgaa gaagttgggc
    25861 gtaccgatca ccccgagcgt gcgcccccga tccttgatcg ctttgagcgc atcgatcatg
    25921 ccttgattct catagcaggc gtcaaactgc gggcgagtca tccccacctg agcggcgatc
    25981 ttgaagatgg gttcatgccg cacctcctgg ctcacccacc tggcctgctg ctccaggaat
    26041 ttgccgaaga gctcgaagta ccgctcctgt ggcgcgcagc ggagtgcgat cgtcgccgca
    26101 cccgattgga gcccgatcgg aaactcgcgc aagatgaacc gcaccttgcc ggtgtcgatg
    26161 tactcgcgct tgagtttcgg gaaggtctcc aggtggaatt tgcggcagta ggggcaggtg
    26221 agggaggcat actcgacgat cgtcaccggc gcgtcgtccc gaccgagcgc catctcgggc
    26281 aacgggcctg gtttcagcac ctcggcgagc gagggattct gaatgacttg ccgcgccggc
    26341 ttgggcgtgc tgaccgcatc gccgaacggg ttgaactcgg gctgatcgat gcttgcgccc
    26401 gagccggctg gcacggcgcc agtcgcctgc agggcctcgc cgagcgacgt ctcgcccgag
    26461 catgccgcca gggcaaaggc ggcgagcagt acgcccgccg ctgcgctctg acgtgagatc
    26521 gatggcagca cccgtgatca gctcttgagc aggggctcga tggccttgtc gaaatcggag
    26581 atcgagttgc caccggacag acgcttgccg ttgatgaaga acgtcggtgt cgagttcacg
    26641 ccgaactgct cgttcgcccg gcggcgcgtc gcttcgatat cggcgagcag cttgtcgtcg
    26701 ccgaggcact tttcgaagct ctccttcgtg aagccagcct gctttgcgat ttcgaaaagt
    26761 ttcgagacag gatcgttgcg ggtgacccat tcatcctgct tttcgaacag cacgtccacc
    26821 atcggcaagg ctttgtcacc agcgcaccgg gcgagcatcg aacccgccgc agcgagatcg
    26881 tcgagcggga actcgcggaa aaccaggcgt gcttggccgg tgtcgatgta cttcttcttg
    26941 agctccggca gcacgcgatt gttgaagttg gcacaatgcg ggcacgtcat cgaggcgtac
    27001 tcgacgatgg tgatcttggc gtcggggttg ccgagcacga ggtccggcaa tggacccggc
    27061 ttcatcagct cctcgacggg gacttccgag ggcccgcgcc gttgcgccag cgccgcagtg
    27121 ataaatggag ccgtcgccat ggcgctcagg ccgatgccgg cggaataaag catcagcgcg
    27181 cgacggctca tgaggttcga ggattcggac aaggcacaac tccttatgat cgacgctatg
    27241 acgagggccg ccaaaaaggc aattttgcga tcgacttaat gcaatctggc gctgaacgcc
    27301 aactgaatga ctgtcacggt tgcgtcaacg gttgggccgc ggctttggat gctgggctgc
    27361 cggggcgttc ccgatgcatg ctggcctgca gtcgagccaa tgcgtgccga agggccgggt
    27421 tctcgatctt cgccagctcc ggcggcgttt cgcccgcagc agcgtcatct tgcggcagca
    27481 ctggggggcg ccacttcggc accgattgtc ccgcgatagg cgcctgcacg aacctcagct
    27541 cggcgacggc gcgatagccg aagctggcgt tgatacgctc gatgatctga tgggtcttgt
    27601 gctgcagctc gatcgcgcgc ggcccgtcga cgcgcaggac gagcgtcgca cctgggcggc
    27661 gctcgttgcc gggctcggtc tcgtcgaaag catcgacgcc gcgcggccac ttcaaccgtt
    27721 caggggcggt gaagctcgcg atatcgctgc cgacgatgtt cgcccagtcc gtcaatagcg
    27781 caagggacgc gaagccatat ttttcgaatg ccttgcgtgt gagctgcgga atatagctcg
    27841 agaccgcgcg ggcagagggc tgcgcgcgca tccgcggatt gagcaccgcc cgcgggggca
    27901 gcggctgggc tcgggaggta cgactgttca cgaacgggac ctgcgcattt caatccgtac
    27961 tggccttgct taccatctgc aggatgattc gcagggcaga cggtgacttc acgtgaacgc
    28021 tgtggcggag gagcaacaga tgcagcccga gcgggaccgc gacgcggccg ctgctctgct
    28081 cgaatggtat gacgctgagc ggcgcgacct gccgtggcga tcacccccgg gcgtcaggcc
    28141 tgatccgtac cgtgtctggc tcagcgaaat catgctgcag cagacgacgg tcaaggccgt
    28201 tatcacttat ttcgatcggt tcctttcgcg gtggcccact gtcgagcaac tagccgctgc
    28261 ggacctcgac gatgtgctcg ctgagtgggc gggccttggc tattacagcc gtgcacgcaa
    28321 cctgcatcgc tgtgcccgtc tcatcgtgga gcgctacgac gggcgtttcc cttcctccga
    28381 agcggagcta ctgaagctcc ccggcatcgg cccttatacg gctgccgcga ttgctgcgat
    28441 cgccttcgat cagcgcgcaa caccggtcga tggcaacgtg gagcgggttg tggcgcggct
    28501 ctttgcggtg accgagccgg tgcctccagc caagcctttg cttcgggagc tcgcagccaa
    28561 gctgacaccg aatcggcgcc caggggattt cgcccaggcc atgatggatc tgggcgcaac
    28621 gatctgcact ccgaagcggc cgtcctgcct gatgtgcccg ctcaacagcc aatgcttggc
    28681 caaggcgagg ggagttcagg gagagcttcc tgtgaagatg gacaagggcg agcggcccat
    28741 tcgattcggc gttgcctttc tcgccttgcg cgaggacgga tgcgtgctcc tgcgcaagcg
    28801 ccccgagacg ggcctcctcg cccgcatgat ggaagtcccc tcgaccgatt ggctggagga
    28861 tccgcgcgtt gtcagcgagg ccctccgggc ggcccccgtg cgggccgact ggtggcgggt
    28921 gccggggcag gtcgcccaca cgttcacgca cttccggctc gagctcatcg tctatcgcgc
    28981 ggtcgtagaa agtgacagca cactcacctt ctgggccgat gccaaccgtt gccgctgggt
    29041 tgctcgccgg gagctggacc ggcaggcttt gccgagcctg atgcgcaagg tgatcgctca
    29101 cggcctgcgc gaaatgtagt gacggcgacc ctcgtcgagt cgccaagccg cagttcggcg
    29161 aaggatgaac ttagcggctc gccgtcatga ccggagcacg ccgcatgagc tcatcgacgc
    29221 gggcacgttc cttttgaaat tccgcaagct ggcgaccggt gagctgccgc tcccggggga
    29281 cctggatctg gagcggattg acgaaccggc tgttgaccag gatttcgtag tgcaggtggg
    29341 gccccgaggc gagacccgtg gctccgacat agccgatgat ttgcccctgc ttgaccttga
    29401 cgccctcctt tatacccggg gcaattcggg acatgtggcc gtaggcggtc tgatagctgt
    29461 tggcatggcg gatgcgcacg tagttgccat atccgccctt gcggtgggct tcctcgacga
    29521 caccattgcc ggccgccagg atcggcgtgc ccaccggggc agcccagtcg atgccggtat
    29581 gcatcctcgc gacgttaata acagggtgga ggcgcattcc gtaaccagaa gtgaggcgca
    29641 cgaaatcgcc ccggaccggc ctgcgcatca ggaagcgctt ggagttgttt ccgtgctcgt
    29701 cgtaatagtc cacgatgccg tccggcgtgc ggaagcgcca gaagcgctgt tgctcgccgc
    29761 ccgaggtgat ggatgtgtag agcagctctc ccggagtgct gtctgggccg tcatcctcct
    29821 tcacgtcgaa gaagaagtcg accatgtctc catgccctac acgccgacgg aagtccgtct
    29881 cgtaagcgtg gacacgcaga atttgcatga tcttctcggg cggcacgcct tccgacagtg
    29941 ccgcataata gatgctcgaa taaaggctgg ccgaatgggc cttgtcgctg tcgttcaggg
    30001 tcgcccgagc aaacttatcg tcattgaagg gttgcgcgct ggctacgaac tcacccgcgg
    30061 tattccggac gaccgtcact ttgtgctcgt gcccttcggc aaagatgctg aaccgaaccg
    30121 gctccatctg ctgcggatcc gacagcgaag gtacgagcgt gacatgcact tcctgacccg
    30181 gcatcaatgc attgtccggg aacgtcggcc gtgccgcctg cagcatcgca ttgatctgcc
    30241 acggctcggc gcctagccgc tgcagaattc gggcgaggct gtcgccacgg gcgacacgga
    30301 taacgcgcac ctcgcttgct tccaggtcat cttcggcgtc gtccggatcg gagaccgatt
    30361 tgaacagcac ggtcgtgttg ggagcgtcct ccgcagctgg ggcgtcgccc gcggcgaggt
    30421 ctggttctgc ctcttgctct gcgacgggat catcgggctg caggcttggc cggatcgcca
    30481 ggttctgcgc cgcttcctct gcacgtgcga caagctcgag aacttcagcc gtatcgagct
    30541 cctggccatc ctcgcccggg agaataccac cgagcagctc cacgaccctg acagagacgt
    30601 ccgatcggcc ctcgccggat gcgccgtccg cttcgcttga ttccgcctcg gcagcgtaga
    30661 gcttcagcgg gttgaacggg gggatctctt cgatcttatc cggaggcact tccgacaggc
    30721 gcgcgacgat ccgcatgtac ggccggatct ggatgaaggg tctgttgtcg cgccggacct
    30781 ggacctgctc atggatgatg tgcttggcgc caacgctgcc gaccgcaacc tgcagccggt
    30841 ccgatttcgg aatcgaccaa ttgaggccgg agtcgcggac gatggccggc tgcggcaggc
    30901 gcgcttctct gaactgctgg agcgccggaa tcacgccacc gctcgtgtcc atgcgatcga
    30961 gtgagccgaa caggacgacg ccgatggcca ccgctccaac gccggcagca aggaaggtgc
    31021 tgaggaccca gcggaaccgt cctccgcgcc gcggttcgtt gaactccctg tcatagatgt
    31081 cgggaacagc cctgcgcgca cggccccgat agacatgcgg cagccgctgc gtccggcttg
    31141 gaagccgtat tgcgcgggcg cgcccgacag gtgcatggcg ctgcgtgttc agcacgccgg
    31201 ttcaatcccc cttgccgcca tctccgcgag acccggtgcg gtcccgttcg atgctcgaca
    31261 acccctcagc aagcgcttgt ctgctgcaaa aaccttgttg atgtttcgag gcgggcaagt
    31321 caatcgactt gttccgacgt ccgcacagtg gcccgcttaa atggcggaac ggcggcagca
    31381 tgagcggcag catggcagag aggccagatt gcagcctgcc actgggggcc gcggactccc
    31441 tcgtggcggc ccagtcaggt gcccccgccg aacctgcggt gtctgaaccc tggggccagg
    31501 ttacccctta agacgccttg tttaccgcct cggagctact atctgagatt agcgccggga
    31561 ggccgttcgg ccaatcagcc gaaattccgg cgaagtctgt tgggctccct tgaatttcgg
    31621 cgcctcggtc ccagattatt gagtgggcga tgcgcctctg tgcgcgtcgg cgcctccgtc
    31681 tgcaccggat gacggctctt gatccctcgg agaaattttt cgagggggct gaatcgggtg
    31741 ttgacaggga cagggggttc cagtagaagc acccttgttg cgcggcgctg acgcctcgct
    31801 gccccggttg gggcgccaac ttccgtctaa ccggcgatct ctccaccggt accgtccgga
    31861 ggggaagtcg ttgtctcgga agacctgcca taggtgggag gcgcccgggg gaactttgct
    31921 ctttgacaag ttaatcgtga agaaagggaa acgcaggcgg cggagtcctg gcgggctcaa
    31981 agcgctcctc gtgggtgccg agagccaaac gagactctgg cgagaactgc gtttcaagcc
    32041 atcaccatgg atgcgcctgg tgggaggtaa ctcttgccat ggcagcgtct tgtgtgaagg
    32101 tgcccgttac gcagtgatcc ttggagtgat tccctccaag ccgtccagaa tctgaactct
    32161 tccaacttga gagtttgatc ctggctcaga acgaacgcgg gcggcagccc taacacatgc
    32221 aagtcgaacg ccccgcaagg ggagtggcag acgggtgagt aacgcgtggg aacctaccca
    32281 gtggtacgga atagcccagg gaaacctggg gtaataccgt atacgcccga gaggggaaag
    32341 atttatcgcc attggatggg cccgcgtcgg attagctagt tggtggggta atggctcacc
    32401 aaggcgacga tccgtagctg gtctgagagg atgatcagcc acactgggac tgagacacgg
    32461 cccagactcc tacgggaggc agcagtgggg aatcttggac aatgggcgca agcctgatcc
    32521 agccatgccg cgtgagtgac gaaggtcttc ggattgtaaa gctcttttgg cggggacgat
    32581 aatgacggta cccgcagaat aagccccggc taacttcgtg ccagcagccg cggtaatacg
    32641 aagggggcta gcgttgttcg gaattactgg gcgtaaaggg cgcgtaggcg gtcccgatcg
    32701 tcggatgtga aaggcctggg cttaacctgg gaacggcatt cgatacggcg gggctcgagt
    32761 gtgggagagg atggtggaat tcccagtgta gaggtgaaat tcgtagatat tgggaagaac
    32821 accggtggcg aaggcggcca tctggaccac aactgacgct gaggcgcgaa agcgtgggga
    32881 gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgcgtgct agacgttggc
    32941 aggcctagcc tgtcggtgtc gtcgctaacg cattaagcac gccgcctggg gagtacggcc
    33001 gcaaggttaa aactcaaagg aattgacggg ggcccgcaca agcggtggag catgcggttt
    33061 aattcgatgt tacgcgaaaa accttaccaa cccttgacat ggtggtcgcg gatcgcagag
    33121 atgctttcct tcagttcggc tggaccggtg acaggtgctg catggctgtc gtcagctcgt
    33181 gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc ctcgtcctta gttgccatca
    33241 tttagttggg cactc
//
LOCUS       DASVDU010000260         8615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_834318, whole genome shotgun sequence.
ACCESSION   DASVDU010000260 DASVDU010000000
VERSION     DASVDU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834318"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VF226_17100"
     CDS             complement(<1..895)
                     /locus_tag="VF226_17100"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX7075757.1"
                     /translation="MQIIAQPPTAPGTQAPPSFAVTASVSLQTRRLRTLKHEDTFAVL
                     NAAGDIAHASEAEGLYHRDTRHLSYFAVGINGTRPLLLSSMVRDDNSALTCEFSNPEI
                     RDETQVQLEQDIIHLSRAEFIWQGVFYQRIAVRNFSNSRKRLKLNFAFAADFVDLFEV
                     RGIHRSARGHFHPPVISSSYVQLAYTGLDNQTRSTQICFDPQPSRIDTNHAEFALDLE
                     ERGYSVVHVAVRCNQSGGRRRPSSAFLSGLRASRNALHQAASQAVSIETSNDIYNEAA
                     RRAIADLYMLMTHMPEGLVPYA"
     gene            complement(900..2015)
                     /locus_tag="VF226_17105"
     CDS             complement(900..2015)
                     /locus_tag="VF226_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7075758.1"
                     /translation="MKIALVSPLHEAVPPILYGGTERVVSTLADELVAQGHDVTLFAS
                     GDSVTAARLIPCCERALRLSPHVRDPLPYHLMMLDEVRRQADDFDIIHFHNDLLHYPL
                     LRPIGRPTVTTLHGRLDLPDLKPFFGRFSDAPLVAISQSQRSQLALGNFVATIYHGLR
                     PDRMKFSPVAKGGYLAFLGRISPEKRPDRAIEIAARVGLPLKIAAKVDRADQEYWEEV
                     IRPKLAGNRSVEYIGEISEREKSSFLGNALALLFPIDWPEPFGIAMIESMACGTPVLA
                     FRCGSVPEVIDHGVTGCIVDSMDEAIAAMDDLLKLDRAAVRRRFEERFTARRMALEYV
                     AVYKQLIESSEETAFLAEPEMSQAAFKQLSITARGNS"
     gene            complement(2261..3469)
                     /locus_tag="VF226_17110"
     CDS             complement(2261..3469)
                     /locus_tag="VF226_17110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7075759.1"
                     /translation="MQKVRSRLPVLKELVLVGAGHAHVTVLRDFGMRPEPGVRLTLVT
                     RQVETPYSGMLPGLIAGHYSFNDAHIDTRPLARFAGAELYRSEAVGIDLAGKRVICRD
                     RPPVRFDVLSIDIGSAPSACVRGVAEHAIPVKPIDGLLARLETARQRVLAAQGRAHVG
                     VVGAGAAGVELMLALHYRLMSEITAAGHPAGGLKFTLFSATDDILPTFPAAVRRYLRT
                     LLQERGIAVLTGSRVDAVEPGAVHIAGGGHVAVDEVFWTTEAEPARWLAETDLALDGR
                     GFIRVTPALQSVSHPDVFAAGDVAMIEGFDLPRSGVYAVRQGPPLARNLRHFLSGRPL
                     MRYKPQREALYLLSTGGKHAVGARNGITFRGRWVWNLKDWLDRRFMARFSTLPDAASC
                     SKLGLDRPRQ"
     gene            complement(3693..6188)
                     /locus_tag="VF226_17115"
     CDS             complement(3693..6188)
                     /locus_tag="VF226_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017268770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX7075760.1"
                     /translation="MVAKLLVGVAIAWSLFQLWYASPLPFIFGVGILNDTEARSVHLG
                     FALFLAYTFYPALRSSPRQRIPYLDWALALAGAFAGSYLFLFYRELALRPGQPTTIDL
                     ITAGAGILLLLEATRRSLGFPMVVVAIVFITYTFVGPYMPEVLQHKGASLSKFLNHQW
                     LVTEGVYGVALGVSTSFVFLFVLFGTLLDKVGAGNWMTQIAVALLGHLRGGPAKVAVV
                     SSALNGVVSGSSVSNVVSTGIFTIPLMKRTGISGVKAGAIEAASSVNGQIMPPVMGAA
                     AFLMVEYVGIPYSQIVKHALLPAVASYIALLYIVHLEALKLGATPLPREEVPTRIRLL
                     RSGIGITSIVITLCVLYYGVIAVQTMFGNNAGWPLAGLGALLYIGTVWFASRHPDLQL
                     DDPSSPLIRLPRAWHVTRTGLDFLLPLFVLLWCLMVEQMSPGLSAFWACAAIIGITLT
                     RKPLLALFRRTDIAEAVHTSIADLSDGLARGARNMISIAVATATAGIVVGTVTLTGLG
                     LMMTDFVEYISGGNVMLMLFLVAIVSLVLGMGIPTTANYILVATLMAPVIVELGAQAG
                     LAIPLIAVHLFVFYFGIMADITPPVGLASFAAAAISREDPIKTGLQGAVYAFRTAILP
                     FVFIFNPALLLIGIDHWWEGALSITTSILAVLVLSAAMMNWFVTRSRLWESAVLVLAC
                     FTLLRPDWWIDQFYPKFVERPAQEFMTRVAEAPANKRLSFVVSGYNIKGDEITKTVNI
                     PLGPVEPAAQRLRNIGLSVTALGDRVSITNVQFGSYAKRIGLEVGYDIVAVLEPADRP
                     SHIWPIGAGFILVGVVALIQRSRLVRAPQPQPA"
     gene            complement(6381..7349)
                     /locus_tag="VF226_17120"
     CDS             complement(6381..7349)
                     /locus_tag="VF226_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7075761.1"
                     /translation="MKRVSLVVSALAAVGLLAGPAAAQQKFVTIGTGGVTGVYYAAGG
                     AICRLLNKDRKSHGIRCSVESTGGSTYNINAIRTGDLDFGMVQSDVHYSALKGVGAFE
                     KDGAFKELRAVFSVHPEPFTVLARREANIKSFEDFKGKRFNIGNPGSGTRTSMEQLLA
                     AMGWTTSDFSLASELKADEHGPALCDNKIDGFFYAVGHPSANIQDPVTTCGAQLVPLT
                     GPVIDKLVEEHPYFAKATIPGGLYAGNPNDIETYGVLATLVTSTNASEETVYQLVKAV
                     FENFDEFKRLHPAFANLDPAKMVKDGLSAPLHDGALKYYKEKGWVK"
     gene            complement(7377..>8615)
                     /locus_tag="VF226_17125"
     CDS             complement(7377..>8615)
                     /locus_tag="VF226_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7075762.1"
                     /translation="ASGGAAVSCLGHGHPAVNEAIKNQLDKLAYAHSSFFTTEVAERL
                     ADNLVEHAPAGISHVYFVSGGSEAIEAALKMARQYFVERGEPQRRHFIARRQSYHGNT
                     LGALAVGGNAWRRQQFAPLLFESYHVSPPYAYREQREGETLEAYGQRLADELEAKIRE
                     LGPETVIAFVAETVGGATQGCTTPPPGYFRRVREICSRYGILLILDEVMSGMGRTGTL
                     HACEQEGIAPDLMAIAKGLGAGYQPIGAVLLSGDVYAAFRDGSGYFQHGHTYMGHPVA
                     CAAALAVQETIIKENLLDNVRQQGQHLERRLRERFANHHHVGDIRGRGLFWAIEYVKD
                     RSTKAPFDPSLGLNARVKREAMSRGLACYAMPGTIDGRHGDHNMLAPPFIVTSSDIDR
                     IVERFGDAVDAAIQSLPKID"
BASE COUNT         1747 a         2626 c         2714 g         1528 t
ORIGIN      
        1 cggcataggg gacaagcccc tccggcatat gcgtcatcag catgtagaga tctgcaatgg
       61 cgcgccgggc ggcttcgttg tagatgtcgt tggacgtctc gatgcttacg gcttgcgagg
      121 cggcctgatg gagagcattc ctcgaggcgc gcagtccgga tagaaatgca gagctgggac
      181 ggcgtcgacc tccggactga ttgcagcgca cggcgacatg gacgacgcta tagccgcgct
      241 cctcgagatc gagcgcgaac tccgcgtggt tggtgtctat tcgcgatggc tgcggatcga
      301 agcaaatctg ggttgaacgc gtctggttgt cgagcccggt gtaggcgagc tgtacgtacg
      361 agctcgagat gaccggaggg tggaaatgtc ctctcgccga ccgatgtatt ccgcgcactt
      421 cgaaaagatc gacgaagtcc gcggcaaacg cgaagttcag ctttaggcgc tttctggaat
      481 tggagaaatt ccggacagcg atgcgttgat aaaatacccc ctgccatatg aattcggcgc
      541 ggctcaggtg tatgatatct tgttccagtt ggacctgggt ttcgtcgcga atctccggat
      601 tggaaaactc acaggtcagc gccgaattgt catcgcgcac catcgagctc agcagcagcg
      661 gccgcgtacc gttgatgcca acggcaaaat acgacagatg gcgcgtatcc cggtgataaa
      721 ggccctccgc ttccgacgcg tgggcaatat caccggccgc attgagtaca gcgaacgtgt
      781 cttcgtgctt gagtgttcgg aggcgtcggg tctgaagcga caccgacgcg gttactgcga
      841 acgatggcgg tgcttgcgtg cccggagccg ttggcggctg tgcaatgatc tgcatatgct
      901 caactgttac cgcgggccgt tatcgacaac tgcttgaatg cggcttggct catctccggt
      961 tcggctagga acgccgtctc ctctgagctt tcgatcagct gcttgtagac tgcgacatac
     1021 tccagagcca tccgcctggc ggtgaaccgc tcctcaaacc tgcgacggac tgccgcccga
     1081 tccagcttca acaaatcgtc catagctgca atggcttcat ccatgctgtc aacaatgcag
     1141 ccggtcacgc cgtggtcaat gacctccgga accgagccgc accggaatgc gagcaccgga
     1201 gtgccgcaag ccatcgattc tatcatagcg atgccgaatg gctcgggcca atcgatcggg
     1261 aacagcagag ccagcgcgtt gccgaggaat gaggatttct cgcgctcact gatttccccg
     1321 atgtattcga cagagcggtt accggccagc tttgggcgga tgacctcctc ccagtactcc
     1381 tgatcggccc tgtcgacctt ggccgcgatt ttcagcggca agccgacgcg ggccgcgatc
     1441 tctatcgccc ggtcgggccg cttctcgggc gagatacggc ccaggaatgc caggtacccg
     1501 cctttcgcga ccggactgaa cttcatccgg tcaggccgca acccgtgata gatcgtggcc
     1561 acgaaattac ccagcgccag ctgcgaccgc tggctctgcg agatggccac cagcggagcg
     1621 tcggagaacc gcccgaagaa cggcttcagg tccggcagat ccaggcgacc gtgcaaggtc
     1681 gtgaccgtcg gcctgccgat cgggcgaagc aaggggtagt gcagaagatc attgtgaaaa
     1741 tgaatgatgt cgaagtcgtc cgcctgacga cggacttcgt cgagcatcat cagatgatat
     1801 gggagcggat cgcgaacatg aggactaagc cgcagagccc gttcgcagca cggaatgagc
     1861 ctcgcggccg tcaccgagtc tccgctcgca aagagggtca catcatgtcc ttgcgcgacg
     1921 agctcgtccg caagggtcga cactacacgc tcggtcccgc cataaaggat cggcggtaca
     1981 gcttcgtgaa ggggggaaac aagagcgatc ttcatgccgc ctcatagacg ccgttgacga
     2041 gttaaccagg ttgtgctggt cccggcgaac agaccgtgtc agccgctcga cacaacgccc
     2101 acgcatggcg aagggttcac ttcagttcgg tcgtaaattg ccacgggttc ttttgcgatc
     2161 gctacgggac cgctggcagg tcttggtgct gggcaccgcc gcgtcgcagc gccagccgct
     2221 cgcgagtcac ctgcctcgtg cagggccgta tttgaagggt ctactgacgc ggcctgtcaa
     2281 gtcccagctt ggagcaggaa gccgcatccg ggagcgtgct gaagcgcgcc atgaaccggc
     2341 ggtcgagcca gtccttgagg ttccacaccc agcggccgcg aaacgtgatg ccgttgcgcg
     2401 caccaaccgc atgcttgccg cctgtcgaca gcagataaag agcctcccgc tgtggcttgt
     2461 acctcatcag cggtctcccg gagaggaagt gacgtagatt gcgcgcgagt ggaggccctt
     2521 gacgaacggc ataaacgcct gagcggggga gatcgaatcc ctcgatcatg gccacgtcac
     2581 cagcggcaaa gacatccgga tgagagacag actggagtgc cggtgtgacg cgaatgaagc
     2641 cccggccgtc gagcgccagg tcggtttcgg cgagccagcg ggcgggctcg gcctcggtcg
     2701 tccagaagac ctcgtcgacg gcaacatgcc cgccgcccgc gatatggaca gcgccgggct
     2761 cgaccgcgtc aacgcgggag ccggtcagca ctgcaatgcc gcgctcttgc aacagcgtcc
     2821 tgaggtaacg ccgcactgcg gccgggaacg tcggcaggat gtcatccgtt gcgctgaaaa
     2881 gggtgaactt cagccctcca gcagggtggc ccgccgcggt gatctcggac atcagcctgt
     2941 aatgcagggc gagcatcagc tcgacgccgg cggcgcctgc tccgacgacc cccacatgag
     3001 ccctgccctg tgctgcgagc acacgctggc gggccgtctc gagccgggcg agcaagccat
     3061 cgataggctt caccgggatc gcgtgttctg cgaccccgcg gacgcaagca cttggcgccg
     3121 atccgatgtc gatcgacagc acgtcgaagc gtacgggcgg ccggtcgcgg cagatcaccc
     3181 gtttgccagc caggtcgatc ccaaccgcct cggaccgata gagctcggcg cccgcgaaac
     3241 gggcgagagg ccgcgtgtcg atgtgtgcat cgttgaagct gtaatggccc gcgatgagac
     3301 ccggaagcat accggagtac ggggtttcga cctgccgggt gacgagggtg aggcgcacgc
     3361 cgggctcagg cctcatgccg aaatcccgca gcacggtcac atgggcatgg ccggcgccga
     3421 ccagcacaag ctccttgagc accggcaatc gtgatcgcac cttctgcatg agcgaatcag
     3481 cgcgcaggaa ccgtctggcc tgtgcggttc ctgcaaaggg aatacgggtt tcctagcaat
     3541 aggttcaggt gcgttcgcaa gcacgcttca agggacgcca caatgtatta aagacacgca
     3601 tgtgccggct cgagagcgaa tggcgaatag gatttcgttc tattcgctat tcgctactca
     3661 ctattcgctc ccaagccggc acacgcggca cgttaggccg gctgaggctg tggcgcgcgc
     3721 acaagccgcg atcgctggat caaggcgacg acgccgacga gaatgaaccc ggccccgata
     3781 ggccagatat gggacggccg atcggccggc tccagcaccg ccacgatgtc atagcccacc
     3841 tcgagcccga tgcgcttggc gtaggagccg aactgtacgt tcgtgatgct gaccctgtcg
     3901 ccgagggccg tcacgctgag tccaatattt cgaagccgct gggcggccgg ctccactggc
     3961 ccgagcggga tgttgaccgt tttcgtgatc tcgtcacctt tgatgttgta gccggaaacc
     4021 acaaacgaca ggcgcttatt ggcgggtgcc tcggcaacgc gggtcataaa ttcctgagct
     4081 ggccgctcca cgaactttgg atagaactgg tcgatccacc agtcgggcct gagcagcgtg
     4141 aagcaagcca gaacgagaac agcagactcc catagccgcg agcgcgtgac gaaccaattc
     4201 atcatggccg ccgagagcac gagaacggcg aggatcgagg tcgtgatcga cagtgcgcct
     4261 tcccaccagt ggtcgatgcc gatcaacagc agtgccgggt tgaatatgaa cacgaagggc
     4321 aggatggccg tacggaacgc gtaaaccgcg ccctgcaggc cggtcttgat cgggtcctct
     4381 ctggaaatgg ccgccgcggc gaaggacgcg agtcccactg gtggcgtgat atcggccatg
     4441 atgccgaagt agaacacaaa gaggtgtacc gcgatgagcg gtatcgcaag ccccgcctgc
     4501 gcgccgagct cgacgatcac tggcgccatc aacgtcgcga ccagaatgta gttcgcggtc
     4561 gtcgggatac ccatgccgag cactaggctg acgatggcga ccaggaacaa catcagcatg
     4621 acgttgccgc cggagatgta ctcgacgaag tcggtcatca tcaggccgag tccagtcagc
     4681 gtcacggtgc cgacgacgat gcccgcggtc gcggtcgcga cggcgatgct gatcatattg
     4741 cgcgcaccgc gtgccagccc gtcggacagg tcggctatgc tcgtatgcac agcttcggcg
     4801 atatccgtcc ggcgaaacag tgcgaggagc ggcttgcgcg tgagcgtgat cccgatgatc
     4861 gccgcacaag cccagaaggc tgacagcccg ggcgacatct gctccaccat caaacaccag
     4921 agcagcacga acagcggcag caggaagtcg agaccggtac gcgtgacgtg ccacgcgcgt
     4981 ggcaatctga tgagcggcga gctgggatca tcgagctgga ggtccggatg acgagaggcg
     5041 aaccagaccg ttccgatgta aagcaaagct cccaggccgg cgagcggcca accggcatta
     5101 ttgccgaaca tggtctgtac agcgatgacg ccgtagtaca gcacgcaaag cgtgatgacg
     5161 atgctcgtga tgccgatgcc gctgcgcagc aggcggatcc tggttggcac ctcctcgcgc
     5221 ggcagcggcg tagcgccgag cttcagcgct tcaagatgca caatgtacag aagcgcgatg
     5281 tacgaggcga cagcgggcag cagcgcgtgc ttgacgatct gagagtacgg gatgccgacg
     5341 tactccacca tcaaaaatgc agccgcaccc atcacaggcg gcatgatctg cccattgacg
     5401 gaggaggcag cctcgatagc gccggctttg acgccggaga tgccggtccg cttcatcagc
     5461 ggaatggtga agatcccggt tgaaacgaca tttgacacgg acgagcccga tacgacgcca
     5521 ttcagcgccg atgacacgac ggcaaccttg gccgggccgc cacgcaagtg gccgagcagc
     5581 gcgacggcaa tctgcgtcat ccagtttccg gcgccgacct tgtcgagcag cgtcccgaat
     5641 agcacgaaca ggaacacgaa actcgtcgag actccaagcg caacgccgta cacgccttcg
     5701 gtgacgagcc actggtgatt gaggaacttg ctgagcgagg cccccttgtg ctggagcacc
     5761 tccggcatgt atggcccgac gaaagtgtat gtgatgaaca cgatcgcgac gacgaccatt
     5821 gggaagccaa gagagcggcg cgtggcttcc agcaggagga gtatgcccgc tcccgccgtg
     5881 atgagatcga tcgtggtcgg ctggccaggc cggagggcca actcgcggta gaacagaaac
     5941 agatacgagc cagcgaacgc ccccgcgagc gccaacgccc agtcaagata aggaatgcgc
     6001 tgccgcggcg aagacctcag ggctggatag aaggtgtagg ccagaaagag cgcaaagccg
     6061 aggtggacag agcgtgcctc ggtatcatta agaatgccga cgccgaagat gaacggcagc
     6121 ggcgacgcgt accaaagctg gaacagcgac caagcgatcg ctacgccaac gagcagcttg
     6181 gcgaccaacc cggagggttc ccggccgccc gtgtcggatt ctgcaaccag cttttcgagc
     6241 tcttccagct ctgcttgaga tttctggtcg tcaggcattg cagaatccgg tgccgttcgc
     6301 atgttcagcc tacgctttta tagcatccga ccggaaacgc gatagtgcgc ctccggtcgg
     6361 acagcatacc caaacgggac ttattttacc cagcccttct ccttgtagta cttgagcgcg
     6421 ccatcatgga gcggcgcaga gaggccatcc ttgaccatct ttgccgggtc caggttggca
     6481 aacgccggat gcagcctctt gaactcatcg aagttctcga acaccgcctt cacgagctgg
     6541 tagacggtct cctcggatgc gttcgtcgat gtaacgagcg tcgcgagcac accataggtt
     6601 tcgatgtcgt tcgggttgcc cgcgtagagg ccaccgggga tcgtcgcctt ggcgaaatac
     6661 gggtgctcct ccaccaactt gtcgatgaca gggcccgtca atggaacgag ctgcgcaccg
     6721 caagtcgtca ccggatcctg gatgttcgcg gacggatggc cgacggcata gaagaaccca
     6781 tcgatcttgt tgtcgcacag cgccggaccg tgctcgtcag ccttcagctc tgaagcgagt
     6841 gagaaatcgc tggtagtcca gcccattgcg gcgagcagct gctccatcga agtgcgggta
     6901 cccgagcccg ggttgccgat gttgaagcgc ttgcccttga aatcctcgaa cgacttgatg
     6961 tttgcttctc tcctggcgag cacggtaaac ggctcgggat gaaccgagaa cacggcccgc
     7021 agctccttga acgcgccgtc cttctcgaag gcaccgacgc ccttcagcgc gctgtagtgc
     7081 acgtcggact gaaccatgcc gaagtccaga tcgccggtgc ggatcgcgtt gatgttgtag
     7141 gtcgagccgc cggtgctctc gaccgagcag cggatgccgt gcgacttgcg atccttgttg
     7201 agcaggcggc agatcgcgcc gccggcggcg tagtagacgc ccgtgacgcc acctgttccg
     7261 atggtcacga acttctgctg tgccgcagcg gggccagcca gaaggccaac tgcggctaga
     7321 gcggatacga ctagactgac tcgtttcatg cgagctctcc cttggtttga cgcggatcag
     7381 tcaattttcg gcagcgattg gatcgctgca tcgacggcat cgccgaaacg ctcgacgatt
     7441 cggtcgatgt cgctgctcgt cacaatgaaa ggtggcgcga gcatgttatg atcaccgtgc
     7501 ctgccatcga tcgtcccggg cattgcatag caggccagtc cacgagacat ggcctctcgc
     7561 ttcactcgcg cattgaggcc gagagatgga tcgaacggcg ctttcgtaga tcggtccttc
     7621 acgtactcga tagcccagaa cagcccgcgc ccccggatgt cgccgacgtg gtggtggttg
     7681 gcgaaccgct cacgaaggcg gcgctcgaga tgctggccct gctgccgcac gttgtcgagt
     7741 aggttttcct tgatgatcgt ctcctgaacg gccagcgcgg cagcgcacgc gacggggtgg
     7801 cccatgtagg tgtgaccatg ctggaaatag ccgctgccat cgcggaacgc tgcatagaca
     7861 tcgcccgaga ggaggacggc cccgatgggc tgataaccgg ctccgaggcc ttttgcaatc
     7921 gccatcagat ccggcgcgat gccttcctgc tcacaggcgt gcaacgtgcc ggtgcggccc
     7981 atgccggaca tgacctcgtc gaggatcagc agaatgccgt agcggctgca gatctcgcgc
     8041 acgcggcgga aatagccggg cggcggtgtc gtgcaccctt gtgttgcacc gccgacggtc
     8101 tcggcgacga atgcgatgac ggtctcagga ccgagctcac ggattttcgc ctcgagctcg
     8161 tccgcgagcc tctgaccata ggcttccaga gtttcgccct cgcgctgctc gcggtaggca
     8221 tacggcgggg agacatgata gctttcgaac agcagcggtg cgaactgctg acggcgccag
     8281 gcattgccgc cgacggccag tgcaccgagc gtgttgccgt gatagctctg ccggcgtgcg
     8341 atgaagtggc gccgctgcgg ctcgccgcgc tccacgaaat actggcgggc catcttcagc
     8401 gccgcctcga tggcttccga tccgcccgag acgaaataga cgtgagaaat gcctgccgga
     8461 gcgtgctcga cgagattgtc cgccaaccgt tcagccactt ccgtcgtgaa gaaggacgag
     8521 tgcgcatagg ccaacttgtc gagctggttc ttgatggcct cgttgaccgc cgggtgccca
     8581 tgcccgaggc acgacactgc cgcgccgccg gaggc
//
LOCUS       DASVDU010000261         2100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_19810, whole genome shotgun sequence.
ACCESSION   DASVDU010000261 DASVDU010000000
VERSION     DASVDU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19810"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..638
                     /locus_tag="VF226_17130"
     CDS             195..638
                     /locus_tag="VF226_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075763.1"
                     /translation="MAAVMAVERESLEYAECELRLIAWGRWSRRMASNLGYPKSSGIA
                     RMIEMVKVFKPAEHSGRELTAKGKETRSMRPLTVEDAPAEVMEIDRLVANLPGWMHAA
                     VSRSYRFRQPDRIAARELRMSREQYTLRREAAVSYLAEKLALRRA"
     gene            complement(995..1189)
                     /locus_tag="VF226_17135"
     CDS             complement(995..1189)
                     /locus_tag="VF226_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075764.1"
                     /translation="MSNHPNRNWRARMRASADQWLTTTEAKVLVEVPLDHRYREQAMR
                     NRIRSAYEAGYRDGRKNDQP"
     gene            complement(1186..1473)
                     /locus_tag="VF226_17140"
     CDS             complement(1186..1473)
                     /locus_tag="VF226_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075765.1"
                     /translation="MNAQQLINRLEQIEITVAQPDERRVISEARQTLMSAVQQVEHAE
                     GAEARAERRQRMGGNVFGATGRVSAEDARAHLRSCMDDALRVARTWGHEIA"
     gene            complement(1486..1770)
                     /locus_tag="VF226_17145"
     CDS             complement(1486..1770)
                     /locus_tag="VF226_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075766.1"
                     /translation="MARKTQLDIVREAIEIQGYIEVYVNGQPRQCREIKVVNGEWVTD
                     ILTSDLCGHVRIKKTKRYVVDQAKRIVANADEIRANREAKEARATWSMMN"
     gene            1888..>2100
                     /locus_tag="VF226_17150"
     CDS             1888..>2100
                     /locus_tag="VF226_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075767.1"
                     /translation="MKRLFAVVLTLICAPALAQGTATLTWELPDQYTNGDPLDPATDL
                     AEIRVYANGALLATTEGTDTQFVTDEL"
BASE COUNT          357 a          636 c          680 g          427 t
ORIGIN      
        1 ctcgcgccga ggtgtcggcg tccgtcagcg ggaacgagtc ttcgggctcg ccggttccgt
       61 cgagcaggct tcaccgccgt cgcgagacag ggacagaagc ggcgaggtcg gcggcatgag
      121 caccggaagc agggcaccgt gatacgcaat cgggcgagcc atcagcgggg cgggcggcgg
      181 agtcggtgtg tgtgatggcg gcggtaatgg cagtagagcg tgaatcgctg gaatacgcgg
      241 aatgcgaact ccggcttatc gcctggggcc gctggtctcg ccgcatggcc tctaatctcg
      301 gatacccgaa aagcagcgga attgcccgca tgattgagat ggtcaaggtg ttcaagcctg
      361 ccgagcattc aggccgagaa ttgaccgcaa aaggcaaaga aacgcgctct atgcggcctt
      421 tgacggtgga ggacgcgccg gccgaggtta tggagattga ccgcctcgtg gccaatttac
      481 cgggctggat gcacgctgcg gtgtcacgga gctaccgatt cagacagccg gaccggattg
      541 cggcgaggga gctgcggatg agccgagagc aatacacctt gcgacgcgag gcggcagtat
      601 cgtatctcgc ggaaaagctt gcacttcgga gggcgtgagg taaaatgcta gtgcccacct
      661 agtggagact ctatactcgg cgggcggtgc ctccctagca cagcgcgacc ttcggcgcgt
      721 tgggagaccg aaggaatctc gctcaggccc aagtccgggc aatgactgcc tgagccccga
      781 gagccccgcc tagcgcgggg ctttcggttt tacgggctgg ccgcactcgc tgcacagatt
      841 gagatgttcg agccgcttca ccaatcggtg tacggtggac tgctctagtc catgagcttt
      901 agctgcagca tagacagtcg agccgtggac taggacgtca cgtgccgcac cctcggcctt
      961 ggatgggccg agggctttca tgcgacgagt gaactcacgg ctggtcattt ttgcggccat
     1021 cacggtatcc ggcctcatag gccgagcgga tacggttgcg catggcctgc tctcggtatc
     1081 tgtggtcgag cgggacttcg acgagcacct tggcttccgt ggtcgtcagc cattggtccg
     1141 ccgaggcgcg catccttgcg cgccagtttc ggttcggatg gttgctcatg cgatctcgtg
     1201 cccccacgtg cgggcaaccc gcaaggcgtc atccatgcac gagcgcagat gcgcgcgggc
     1261 gtcttcggcc gatacgcggc cggtcgcgcc aaagacgttg ccgcccatgc gctgccgacg
     1321 ctcggcccgc gcctcggcac cttcggcatg ctcgacctgc tggacggcgc tcatgagcgt
     1381 ctgacgcgcc tcgctgatga cccgccgctc gtcgggttga gcgactgtga tctcgatctg
     1441 ctccaatcga ttgatgagct gctgtgcgtt catggctgtc tccggttagt tcatcatcga
     1501 ccaggtggcg cgcgcctcct tggcttctcg attcgcccga atctcgtccg cgttggcgac
     1561 gatccgcttc gcctggtcga cgacgtagcg cttcgtcttc ttgatgcgca cgtggccgca
     1621 gaggtcgctt gtaaggatgt ccgtcaccca ctcgccgttg acgaccttga tttcgcggca
     1681 ctggcggggc tggccgttta cgtagacttc gatgtacccc tggatctcga tcgcttcgcg
     1741 gacgatgtcg agctgggtct tgcgtgccat tctggtctcc gcccctgatc cccgaggcgc
     1801 ggttggctgg tatcagccta tgagtccatt ctacacactt gcgtctctcg cgcaagtaga
     1861 tattcataca gtagttctga ggtgcctatg aaacgcctat tcgcggtcgt cctgaccctg
     1921 atttgcgcgc cggcccttgc tcaaggcacg gccacgctta cgtgggagct gccggaccaa
     1981 tacaccaacg gcgatccact tgaccccgca acggacctgg ccgagatccg tgtctacgcc
     2041 aacggtgcgc tgctggcgac gacggagggt acggatactc aattcgtgac cgatgagctg
//
LOCUS       DASVDU010000262         7668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_86183, whole genome shotgun sequence.
ACCESSION   DASVDU010000262 DASVDU010000000
VERSION     DASVDU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86183"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..897)
                     /locus_tag="VF226_17155"
     CDS             complement(154..897)
                     /locus_tag="VF226_17155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX7075768.1"
                     /translation="MARLSDRVALVTGGTRGIGLAIAKAYLDEGASVMIAGTTEKSLR
                     SADVATLGSSRMATVAGNLESSETPDALVSATVARFGSLDILVNNAGILGPSDLQELD
                     TEDWDRVLNVNLRSAFFTARAAAAHMRGRGGCIINLSSVAAQIGGTATSPAYVASKAG
                     LIGLTRSLARHLAPLGIRVNCIAPADIETDMTADWPTEVRQRLTAMTPLSRFGEPSEV
                     AGLALYLADASAAFITGQTFNVNGGLYMG"
     gene            complement(903..1760)
                     /locus_tag="VF226_17160"
     CDS             complement(903..1760)
                     /locus_tag="VF226_17160"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7075769.1"
                     /translation="MLLVRYSTERTGPTRPGMAVGDSVYDLSAKYGSISGFLAAHPDG
                     WTESSVPLASLRKFDRGAVRLGPPMDDSASIYLVGANYKKHAEEAGLDVPQTPVIFQK
                     PFTALVGPTDPIVIPPISKQMDFEGELAVVIGRMARRVSAEQAKRHVAGLTIVNDVTA
                     RDLQWVNLGKHRIVDWLSSKALDESTPVGPGIASRAAVSDPHRLRLTTRLNEDLMQDS
                     DTSLMVFSIWELIEFLSARVTLRPGDLLSTGTPVGVGGFRKIFLKHGDRVRVEIEGIG
                     LIDNPVKAE"
     gene            complement(1772..3997)
                     /locus_tag="VF226_17165"
     CDS             complement(1772..3997)
                     /locus_tag="VF226_17165"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7075770.1"
                     /translation="MQIDDAFTTEATPGVDPVTFEVLNHRLLSITEEMGIQYMRCSGS
                     NVLITGNDAATAIMRADGALVAVGPYIVTQGNVLPLIVEATKRLSGDVGINDGDIFIC
                     NDPYLGAIHAPDFATVAPIFDNGRIVAWIGASGHQLDTGGMDPGGFSVKAVDIHQEGL
                     RIPPVKIVDRGIVREDVMRWIINQVRDPLVGLDVKGQIAALNNGRQRVLELIKTWGIG
                     AVQGVMEQSIAYARDKLRQRLRELPDGQWRDIQYIDHDGHESKIYRIVCTMTKRGERV
                     TFDFRGTSPNARGLINCTYSGLQAAVLSSMYINLCWDIPWNRGVRDCVEIIAESGTVN
                     NCDYPAPCAMATISAVIVTIDVTWRCLSNLLLTSASHHAQAMAVWSGTSMAPIFAGRS
                     QHGFAFSATEMSHFGGGGGARTYADGVDTAGIVFNTTPNMPNIEDQEAEYPVLYLFRK
                     HLRDSGGPGLHRGGRSAELAYMVYDAPEEKLEGLFAGTGAEMPNAIGVSGGMPGSAIR
                     IARFVGTGLNRRVQQKLPLPASLPDVPGKFEILASKHERTPIDAGDVWYHSWQAGGGY
                     GDPLRRDPGRVAEDVSRGAVSVEAAREIYGVALGPDNSVDAAETQTLRAVHRKTRLDR
                     SREPRPGDAVMRYSGQASSRFGTALTLDLANGAIRCSHCDTALGAPGHDILPRLREWS
                     SPLNAAGPVRGEDYDQGRFQLRHLLCPQCGSAVDVHVAHEGAPWPSADVVIHGARPEI
                     S"
     gene            complement(4005..6074)
                     /locus_tag="VF226_17170"
     CDS             complement(4005..6074)
                     /locus_tag="VF226_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7075771.1"
                     /translation="MYTVAVDIGGTFTDVVAFDRDSKRMAIGKSLTTPDDLQRGVFDA
                     LDDAAQEFKIGVRDLIGGSERLVHATTQSSNAVFALTGARTAVLTTRGFEDTLIIMRA
                     TGRVAGLSVFERHHYRNTQKPTLLADERDIVGVPERTDYLGRIVTRLDEEAVREAARK
                     IRDGGYGAVAVAYLFSHKNPEHERRTAEIIAKEAPGLYISLSSDVAPVLGEYERSATA
                     LFNAYVGPVIVGYLDRLERTLFESGLRQKLLVVQANGGIATAGQVVPVFTIESGPAAG
                     VVGAAHLANELGHKNVIATDVGGTTFKVAIIRDGAWTYSKETVLNQYQLRLPMVDVAS
                     IGAGGGSIAWIDGSRLRVGPQSASSDPGPACYGLGGNAPTVTDADVVLGYVAPERFLA
                     GRMSLRKDLAEQAIKNQLASPLFAGDVVSAAAGIRQVIDSQMADLIRKSTLERGYDPR
                     DFVMMAYGGCGPVHAASYSAEVGVREIIVPFFATAHSAYGAAISDIRFSLAYSDPLTL
                     PVAPARLEDIYRAMEERGQVLLGEADVSPDARKYARWVEARYRRQVHYVRIPVPSRLD
                     PAAVDALSRSFHAEYERLFGEGSGLRDAGIELVNYGVEAIGTVDKPTTAEVQPGSLAE
                     PRAVRATYCPRRRGMVETSIYDGPALPAESLIEGPAVIEHPGTNIVVFDGQKARIDRY
                     GHTHILL"
     gene            6342..7100
                     /locus_tag="VF226_17175"
     CDS             6342..7100
                     /locus_tag="VF226_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075772.1"
                     /translation="MSATFTAECTSGNYRYIPGVFQYSAGVAADSGYEIVRVRLRGVV
                     PLADGFRLVEEYLKMVARPLASLCACELRSPAPFTDEGFKAFNELYVGTLESWGIYKD
                     GVNPVARSNVCPAVNAPATPSLYAFSYTLPSKQTVFNTFQVAGSAEAPEGKQNYRDHI
                     VRRGEHSVDALLDKARFVLGEMERRLGLLGRSWKDATGTQLYTVYDPHPAVMQEIAKR
                     GAMASGLTWHFCRPPVVDLDYEMDVRAIRSEVVI"
     gene            complement(7254..7643)
                     /locus_tag="VF226_17180"
     CDS             complement(7254..7643)
                     /locus_tag="VF226_17180"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7075773.1"
                     /translation="MQKRQEYMIEGLMPPISHYCDAVRWGDLLFVSGVAPVDAKGKVV
                     SDDPAAQTRQIFLNMKAILDAAGASFADVLKVTVYLTDVEDRKKINPVRQEFFGAARP
                     ASTLIGVKALAIPEMKVEIEAVVGLRA"
BASE COUNT         1441 a         2321 c         2390 g         1516 t
ORIGIN      
        1 ggtaaagggc tccgactttc acttgggcaa gcgctgggct tgccgcggcg accccaagca
       61 cacacgagat cgcgatgagc aacctgctga ctgacatatt gcacctcctc caagtggcgc
      121 caaaaatatt cgtacatgga ccgcggcgcg cggtcagccc atgtagagac ccccgttcac
      181 attgaaagtc tggccggtta tgaatgcggc gctcgcatcc gcgagataca gtgcgaggcc
      241 ggcaacctcg ctcggctcgc caaatcggga cagaggcgtc atggcggtca gacgctgtcg
      301 gacctctgtc ggccaatctg ccgtcatgtc ggtttcaata tccgccggcg caatgcaatt
      361 gacgcggatt ccaagcggag ccaagtgacg tgcaagggat ctggtcaaac ctatcaggcc
      421 ggccttcgac gcaacgtacg cgggactcgt cgcggtgccg ccgatctgcg cggcgactga
      481 cgaaagattg atgatgcagc ccccgcgccc gcgcatatgc gcggccgccg ctcgagcggt
      541 gaaaaacgcc gatcgaaggt ttacgttcaa gacgcggtcc caatcctcgg tatccagttc
      601 ctggagatcg gaagggccga ggattccggc attgttcaca aggatgtcca ggcttccaaa
      661 tcgggccacc gttgcgctca ccagggcatc cggggtttcg gaagattcca ggttgccggc
      721 gaccgtcgcc atccgggagg acccaagggt tgccacgtcc gccgagcgca gcgatttttc
      781 ggtcgtgccg gcgatcatca ccgatgcgcc ttcgtccagg tacgctttgg caatcgccag
      841 cccgatgccg cgagtgccac cggtaacgag tgcgacacga tctgacaggc gcgccatatg
      901 cgctattcag ccttgaccgg gttgtcgatc agcccgattc cttcgatctc cacccgcacg
      961 cggtcaccgt gcttcaggaa gatcttgcga aatcctccga cgccgaccgg ggttcccgtg
     1021 gatagaaggt cgcccggtcg gagcgttacc cgcgcagaga ggaactcgat gagctcccag
     1081 atcgagaaga ccatcaacga ggtatccgag tcctgcatca gatcctcgtt gagccgagtg
     1141 gtgagtctca ggcgatgagg atccgagacg gcagccctgc tggcgatgcc cggcccgacg
     1201 ggcgtggatt catcaagcgc cttggaggat agccagtcga cgatcctgtg cttgcccaga
     1261 ttcacccatt ggagatcgcg cgcggtcacg tcgttgacga tggtcaggcc tgcgacatgc
     1321 cgctttgcct gttccgccga cacccgtcgc gccatgcgac cgataacgac tgcgagttcg
     1381 ccttcgaagt ccatctgttt gctgatcgga gggatgacaa tcgggtccgt ggggccgaca
     1441 agcgcggtga aaggcttttg aaatatgacc ggtgtctggg gaacatcgag ccccgcctcc
     1501 tccgcgtgtt tcttgtagtt cgcgcccacc aggtagatcg aggcactgtc atccatgggt
     1561 gggccaagcc gaactgcgcc tcgatcaaat ttccgcaagg acgcaagcgg cacactgctt
     1621 tcggtccacc cgtccggatg ggccgccaga aatccggaaa tcgagccgta cttggcgctc
     1681 aggtcataga ccgaatcacc gaccgccatg cccggacgcg tgggacccgt gcgttcggtc
     1741 gagtatctga cgagcaacat gtttcctcca cctagcttat ttcaggtcgg gcgccatgga
     1801 ttacgacgtc tgcagatggc catggagcac cttcgtgcgc gacgtgcaca tcgactgcgc
     1861 tgccgcattg cgggcaaagc aagtgccgca gttgaaagcg gccctgatcg tagtcttcgc
     1921 cacgaacggg gccagcggcg ttaagcggcg agctccactc tcgaagtctg ggcagaatgt
     1981 cgtgtcccgg ggcaccgagc gcggtatcgc agtgcgaaca gcggatcgcg ccattggcga
     2041 gatcgagcgt cagcgcggtt ccgaagcggg agcttgcttg accggaatag cgcatgacgg
     2101 catcgccggg ccgaggttct cgcgatctgt cgaggcgcgt ctttcggtga acggccctga
     2161 gggtttgcgt ttcggctgcg tcgaccgaat tgtcgggacc gagtgcaacg ccgtagatct
     2221 cccgagcggc ctcgacggac accgcgccgc gggaaacgtc ttcggcgacc ctgcccggat
     2281 cccggcgcaa cgggtccccg tagccgcctc ccgcctgcca gctgtggtac cagacgtcgc
     2341 ctgcatcgat cggtgtgcgt tcgtgtttcg acgcgagtat ctcgaacttg ccggggacat
     2401 cgggcagcga agcgggaagg gggagcttct gttgcacacg cctattgagg cctgtgccga
     2461 cgaatcgggc gatgcggatt gccgagcccg gcatcccccc cgagacaccg attgcattgg
     2521 gcatttcagc gcctgtgccg gcaaaaaggc cctctagctt ttcctccggt gcgtcgtaca
     2581 ccatgtatgc cagctccgcg gaccgacctc ctcggtgcag accgggacct ccggaatcac
     2641 gcaaatgctt gcggaagaga tagaggaccg gatattccgc ctcctggtcc tcgatgttcg
     2701 gcatgttcgg cgtggtgttg aagacgatgc ctgcggtatc aacaccatct gcgtaagtac
     2761 gcgcgccgcc gccgcctcca aagtgactca tctcggtggc agagaacgcg aacccgtgct
     2821 gactgcgacc ggcgaagatg ggcgccatgg aagtacccga ccacactgcc atcgcctgcg
     2881 cgtgatgcga agcgcttgtg agcagcaggt tggagaggca tcgccaggtg acatcgatcg
     2941 tgacaattac cgcggagatc gtcgccatgg cgcaaggcgc agggtaatcg caattgttga
     3001 ccgtgcccga ttcggcgatg atttcgacgc agtctcgcac gcctcgattc catgggatgt
     3061 cccagcagag gttgatgtac atcgaggaca gcaccgccgc ctgcaggccg gaataggtgc
     3121 agttgatcag gcctcgcgca ttggggcttg tgcccctgaa atcgaaggtg acccgttcgc
     3181 cccgcttggt catcgtgcac acgatccgat atatcttcga ctcgtgacca tcatggtcga
     3241 tgtactgaat gtcgcgccat tgaccatccg gcagctcgcg caatctctgc cgcagtttgt
     3301 cgcgcgcgta ggcaatcgac tgctccatca cgccttgaac cgcgccgata ccccacgtct
     3361 tgatgagctc gagcacgcgc tgtcggccat tgttgagagc agcaatctga cccttcacat
     3421 ccagaccgac gagcggatcg cggacctgat tgatgatcca ccgcatgacg tcttcgcgga
     3481 cgataccgcg gtcgacgatc tttacgggag ggatacgcaa gccctcctgg tgaatatcga
     3541 cggcctttac cgagaagccg cctggatcca tgcctcccgt gtccaactga tgccccgacg
     3601 ctccgatcca ggcaacgatc ctgccattgt caaaaatcgg ggcgaccgtc gcgaagtcgg
     3661 gcgcgtgtat agcgccgagg tacggatcgt tgcagatgaa tatatcgccg tcgtttatgc
     3721 cgacatcgcc cgacaagcgc ttcgttgcct cgactatcag cggcagaaca ttgccctggg
     3781 tgacgatgta cggacccacc gcgaccaatg ccccgtccgc ccgcatgatc gcggtcgcag
     3841 catcgttgcc cgtaatgagg acgttcgagc ccgagcagcg catgtactgg atgcccattt
     3901 cttcggtgat gctgagcagg cgatggttga gcacttcgaa ggtgacgggg tcgacccccg
     3961 gtgttgcttc ggtcgtgaat gcgtcatcaa tttgcatggc ggcgctagag aagtatgtgg
     4021 gtgtgtccgt accggtcgat acgggctttc tggccgtcga acacgacgat gttggtgcct
     4081 ggatgctcga tgaccgcagg tccttcgatc aggctttccg cgggcagcgc ggggccgtcg
     4141 taaatcgagg tctcgaccat gccgcgtcga cgcggacagt acgtggcgcg cactgcgcgc
     4201 ggctctgcca gcgatccagg ctgtacttca gccgtggtcg gcttatcgac tgtgccgatg
     4261 gcttccactc cataattgac aagctcgatt cccgcatccc gcaggccgga gccctcgcca
     4321 aagaggcgtt catactcggc gtgaaaggag cgcgagagcg cgtcgacggc agccgggtcg
     4381 aggcgcgatg gcaccgggat gcgcacgtag tggacctggc ggcggtaacg ggcctcgacc
     4441 cagcgagcgt actttctcgc gtcgggcgat acgtctgctt cgcccagcag gacctggccg
     4501 cgctcttcca tggcccgata gatgtcctcg aggcgcgctg gggccacggg aagcgtcagc
     4561 ggatcggagt acgcaaggct gaatcggata tcggagatcg ctgcaccata ggcggagtgt
     4621 gccgtggcaa agaacggaac gatgatttcg cgcacgccca cctcggcgct gtacgaggcg
     4681 gcgtggaccg ggccacagcc gccataggcc atcatcacga aatctcgcgg atcgtatccg
     4741 cgctcgagtg tagatttgcg gatcaggtcg gccatctggc tgtcgattac ctggcgaatc
     4801 cctgccgcgg ccgacacgac gtcacccgcg aagagcggcg atgccagctg attcttgatg
     4861 gcttgctctg cgagatcctt ccgcagcgac atccgccccg caagaaaccg ttcgggtgcg
     4921 acgtaaccta gcaccacgtc ggcgtctgtc actgtcggcg cgttgcctcc aagcccatag
     4981 caggcgggac cggggtcgga ggaggcgctt tgaggcccca ctcgcaatcg agacccgtca
     5041 atccatgcga tcgagccgcc gccggcgccg atggacgcga catccaccat cggcagacgc
     5101 agctgatact gatttagcac cgtttccttg ctatacgtcc acgcgccatc gcgaatgatc
     5161 gcgactttga aggtcgtgcc tccgacgtct gtcgcgataa cgttcttgtg gccgagctca
     5221 ttggccaggt gcgccgcgcc cactacaccg gccgcagggc cggactcgat ggtgaatacg
     5281 ggtacaacct gtccagccgt ggcaattccg ccgtttgcct ggacaacgag gagtttctgg
     5341 cgaaggccac tttcgaaaag cgtgcgttcg agccgatcga ggtagccgac gatcacggga
     5401 ccgacgtagg cgttgaagag ggcggttgcc gagcgctcgt attcgcccaa taccggggcg
     5461 acatcggacg atagcgagat atacagtcca ggagcctctt tcgcgatgat ttccgcggtg
     5521 cgacgctcat gctccggatt cttgtgggaa aagaggtagg cgacggcgac cgccccgtat
     5581 ccaccgtcac ggatcttcct cgccgcctcg cgcaccgctt cctcgtccag tcgagtcacg
     5641 attcggccaa ggtagtcggt gcgctccgga acgccgacga tatcccgctc gtcggcaaga
     5701 agcgtcggct tttgagtatt tctgtagtga tgccgctcga agacactcag gcccgcaacc
     5761 cggccggtcg cgcgcatgat gatcagcgtg tcttcgaagc cacgggtggt gagtaccgcc
     5821 gtgcgtgcgc cggtcaacgc gaagaccgcg ttgctcgact gggtcgtagc gtgtaccaga
     5881 cgctcggaac cgccaatgag gtctctgacg ccaatcttga actcttgcgc ggcatcatcg
     5941 agcgcgtcga atacgccgcg ctgtagatcg tcaggcgtgg tcaacgactt tccgatcgcc
     6001 atccgcttgc tgtcgcgatc aaatgcgacg acgtcggtaa aggtcccccc aatgtcgacg
     6061 gcgacggtgt acacgctgtt atgtcctgtc caaggttcga tggagtttag tgtttttagt
     6121 gaaatcttcg tcactttgat gtgcagtgtc aaggacgggc gatctcaaga tgaagatccc
     6181 aactgcgtaa cgagcggcga gacacgcgct cgtgcgcgcg atattgcgct gcgcaacgaa
     6241 ggccggactt tcgatttgga gcgaggaggt tcgattgcgc gcgcgttcaa cgcgtcggct
     6301 tcgtctatac tcggcgccct tgaatcgctt cgaatttgga catgagcgcg accttcacgg
     6361 ctgaatgcac gagcggcaat taccggtaca ttcccggagt attccagtac tcggcgggtg
     6421 tcgcggccga ctcggggtac gaaatcgttc gggtgcgtct aagaggcgtt gtgccgctcg
     6481 cagatggatt tcgtcttgtc gaagagtatc tgaagatggt cgcgcgccca cttgcttcat
     6541 tgtgcgcgtg cgaacttcgt tccccggccc cgttcacgga cgaaggattc aaggctttta
     6601 acgaacttta cgtcggtact ttggagagct ggggcatcta caaagacggg gtcaatcctg
     6661 tggcgcgttc gaacgtgtgc cccgcggtaa atgcacccgc gacgccgagt ctctacgctt
     6721 tttcgtacac gctcccaagc aaacaaaccg tgttcaacac gttccaggtc gccggaagcg
     6781 cagaggcgcc cgaaggaaaa caaaattacc gggaccacat cgtgcggcgc ggcgagcact
     6841 ctgtggacgc gctgttggac aaggcccgct ttgtactcgg cgaaatggag cgacgactcg
     6901 gtcttctcgg tcgctcgtgg aaagatgcga ctggaacaca gctttacact gtctacgatc
     6961 cgcatcccgc ggttatgcag gagattgcta aacgtggcgc tatggcttct ggtctgacgt
     7021 ggcacttttg ccgaccgccg gttgtagatc ttgattacga aatggatgtg cgtgcaatcc
     7081 gaagcgaggt tgttatatag caggcgagcg cacatgccgg gtcgtcacgc ataggcacgc
     7141 caggaccacg ttgcggctca gacatgcgcc gctagtctgg gggcgttcct gcgaagctag
     7201 gtggtcgccc ttcggcggta tgacgtggat gaagatgtgc gtcccgggcg aggtcaggct
     7261 cgcaggccga ccaccgcctc gatctcgact ttcatttccg gaatcgcgag cgccttgacg
     7321 ccgatcaggg tgctcgcggg gcgggcggcg ccgaagaatt cctggcgcac ggggttgatc
     7381 ttcttgcggt cctcgacgtc ggtgaggtag acggtgacct tgagcacgtc ggcgaagctc
     7441 gcgccggcgg catccagaat ggctttcatg ttgaggaaga tctggcgcgt ctgcgcggcg
     7501 gggtcgtcgc tcacgacctt gcctttcgcg tcgaccgggg cgacgccgga gacgaacagc
     7561 aggtcgcccc agcgcacggc gtcgcagtag tggctgatgg ggggcatgag gccttcgatc
     7621 atgtattcct ggcgcttttg catggtgttt cctccttgtt atgcggac
//
LOCUS       DASVDU010000263         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_265937, whole genome shotgun sequence.
ACCESSION   DASVDU010000263 DASVDU010000000
VERSION     DASVDU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265937"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..1073)
                     /locus_tag="VF226_17185"
     CDS             complement(225..1073)
                     /locus_tag="VF226_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075774.1"
                     /translation="MSKHIVVSTLCAAVSLCLNFTAASAHESRWVRWPAAAGVVQHII
                     TNDSNQTSEMTVSSLLSGSCDRSARSTYPANDKRFSSRVSLELQPRAWVAIWALIAER
                     SIGCRAELVLAGAEGSSALTSTALKPEVEFVEVLPYEAGEVHIDTSVVLEDLLNAFEP
                     QGTNLQVQLRVRNISSDLRTYSVIGRHLECGAGTNFDWVVGPGEAPVQISSGPIVVKP
                     KAAVVFSQRLRGSGDSSVCTASFTIAEARRPYEAPHVEHEEPPVWHAIKNVSVRLSPT
                     VKAYYR"
     gene            complement(1542..2108)
                     /locus_tag="VF226_17190"
     CDS             complement(1542..2108)
                     /locus_tag="VF226_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075775.1"
                     /translation="MTTTNGTLVTSHQSRGFTDSERQLVSKLARPSLLGRLFPRSVFG
                     TRALQADLASARVDVWTVVADTFAVLDPPADFGFVVFIDLGNSTLMGLAGGDWVVDSS
                     ISSYPDKVAEVFATPGQFVRGFRLERLPASGMVLRFDVLDASTVAARYRVAKKDLDYF
                     GQSIVFPGRLESLRDDLNRHLASARMTA"
     gene            complement(2095..2631)
                     /locus_tag="VF226_17195"
     CDS             complement(2095..2631)
                     /locus_tag="VF226_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075776.1"
                     /translation="MTNLRGSINGYAENAVDYWIEQDAWYSPVMGTFAMSMTAEYDIQ
                     TVTSLAGGGFGSLAMRTGQLLRPLANAGPFPRLARAYWDRFGPAGGRFGRSLHHWFFR
                     QDNRWVPAAIKNGNWNLVELPGLVRTPFGGLNQWMGLSRSPWASVTEWAIRIGILSAP
                     AGGAYVGGTIGIEMHDDD"
BASE COUNT          609 a          828 c          794 g          544 t
ORIGIN      
        1 gcaaattgaa gggctttgcg catctggcaa aggtcatcgc tggcgtgcgc ttcgtcgatg
       61 gtgtcgagaa aatcgaggat caagaggtca gcagagccgc cgcctgatca gacttcgtac
      121 accagaattg acgatagctc accgccggag tgccaccacc tcacggtttc ggatatggca
      181 atggaattcc tcgactactg caccaccatt ccacgagatg gatgtcaccg atagtacgct
      241 ttgaccgtgg gcgagaggcg tactgataca ttcttgattg catgccaaac gggcggttct
      301 tcatgctcca catgtggagc ctcatagggt cgccgcgcct cagcaatcgt gaatgaagcc
      361 gtgcatacgc tagagtcgcc agagccacgt agccgctggg agaaaactac ggcggctttc
      421 ggtttcacga ctattggtcc ggacgaaatt tgcacaggtg cttcccctgg tccgaccacc
      481 cagtcgaagt tggttccggc tccgcattcg agatgacgcc caatgactga atacgttcgc
      541 agatcggatg aaatgtttcg aacacgaagt tgcacttgaa gatttgtccc ctgaggctcg
      601 aaagcgttca ataggtcctc aagtacaacc gatgtgtcga tgtgtacttc acctgcctcg
      661 tagggcagta cttcgacgaa ctcgacttca ggcttaagcg ccgttgatgt gagggcactt
      721 gacccctccg caccggcaag aaccagctcg gccctgcatc cgatactccg ctccgcaatc
      781 aacgcccaga tcgcgaccca cgcccgaggt tgaagttcaa gcgaaactct cgaggagaat
      841 cttttgtcgt tcgccggata tgttgatcga gctgatctgt cgcagcttcc gctaagtagc
      901 gatgaaaccg tcatctcgct cgtctggttg ctgtcattgg tgatgatgtg ctgcacgacg
      961 cccgccgcag ctggccagcg aacccagcga gactcgtgag cgctggctgc agtgaaattg
     1021 agacagagcg agaccgcggc acagagggtg ctgacgacga tgtgcttgct catcgcgaac
     1081 atcctcctcc gtaccttatg cattgattgt acgcgtgatt agtgtaatcg cgtccgggtt
     1141 caagtcggaa cctcggttcc atgcgcagat cccattggag cctatagacg acgctgtgcc
     1201 atcggtccaa atcggcctgg gtccaagtcg gcctgccatg ggcgcgaatg taccgtatgt
     1261 ctgcagttat gttcgcatgc ccgcccgaag ccccttgagg ggatgggggt gtagaacaac
     1321 tccagcacgc gcgtcgtcgc gcgctgaccg actcgttcca ctgagatcac cgaagctgca
     1381 acgtgtcgca tgccactgcc gcagcccggc gcgctgtttg cgttgcgggc ggcagccgca
     1441 gtggccaagc gaacgctgcg cagagcgaaa agcgacgcgc ggctcaagcg aagcgcaact
     1501 cacgcaagca gcgtggcggg cggtcggaga gccgcgcggg atcaagccgt catccgtgca
     1561 gaggccaagt gccgattcag gtcatctcgc agcgattcca gccgaccagg gaagacgatc
     1621 gattgaccga agtagtcgag atccttcttg gcaactcggt agcgcgcagc gacagtcgat
     1681 gcatcgagaa catcgaaccg caaaaccatc ccgctcgctg gcagccgctc gagacggaag
     1741 cctcgaacga actgccctgg tgtcgcgaag acctcggcaa ccttgtccgg gtaactgctg
     1801 atggagctgt caaccaccca atccccgcca gcgagtccca tcagtgtcga gttacccaag
     1861 tcgatgaaca cgacaaaccc aaagtcggcc ggtggatcca acacggcaaa ggtatcggca
     1921 accaccgtcc aaacatcaac acgggcacta gccagatctg cttgaagtgc ccgcgtgcca
     1981 aagacacttc gaggaaagag tcggcccagt aggctaggcc gagccagctt gctaacgagc
     2041 tgccgctcgc tatccgtgaa ccctctgctc tgatgactgg taaccagagt gccgttagtc
     2101 gtcgtcatgc atttcaattc cgatcgtgcc gccaacgtat gcgccgccag caggtgcgga
     2161 caatattccg attcggatag cccattcggt aactgatgcc caaggggaac ggctcagacc
     2221 catccactga ttgagtccgc caaatggcgt gcgaacgaga ccgggtagtt caaccaggtt
     2281 ccagttgcca ttcttgatgg cggccggaac ccaacggttg tcttgcctaa agaaccagtg
     2341 atgcaaactg cgcccgaagc gtccgccggc aggaccgaac cgatcccagt acgctctcgc
     2401 gaggcggggg aagggacctg cattcgccag tggacgtaag agctgacccg tcctcatagc
     2461 cagcgacccg aaaccgccgc ccgcaaggct tgtgacggtc tggatgtcgt actccgctgt
     2521 catggacatt gcgaacgtgc ccatcacggg tgagtaccac gcgtcctgct cgatccaata
     2581 atcgacagcg ttctcagcgt atccgttgat gctcccgcgc aggttcgtca tccaagaaga
     2641 cgggttcgcc cagctcccac ccgctgtgct aacaggtggg tccgaggcgg cgcggcagct
     2701 ttaaagagcg attttcctga agcgatcagg aagttgcggc gagtgtgccg cagcgctttt
     2761 gcatcggcaa ggctt
//
LOCUS       DASVDU010000264         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1041962, whole genome shotgun sequence.
ACCESSION   DASVDU010000264 DASVDU010000000
VERSION     DASVDU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041962"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..662)
                     /locus_tag="VF226_17200"
     CDS             complement(276..662)
                     /locus_tag="VF226_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEX7075777.1"
                     /translation="MAAMAEKPARYPYQAPIDAYGNGGFRFAEMSHRGSILCLPSGIY
                     AWDISGADAFSLDAFAQVFAEKERIDFLLLGTGRTQIFPSREIRNGFVAAGLGLEPMD
                     TGAACRTYNVLLAEHRRVAAALLAVD"
     gene            complement(684..1649)
                     /gene="secF"
                     /locus_tag="VF226_17205"
     CDS             complement(684..1649)
                     /gene="secF"
                     /locus_tag="VF226_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7075778.1"
                     /translation="MFLVPNFKGINLFPADARIPFMRFKGVCLIGSFIAMLLSIVLIA
                     TVGFNYGVDFKGGSMIEVQSKSGPVDLASVRSKLGSLGLGEVQIQEFGAPNDILIRVE
                     EQPGGEEAQPQAVNKVLAALGDDFVQRRVEIVGPAVSSELRTTGIIAVLVSIIGITAY
                     VWFRFEWQFAVGAVLALAHDVLLVAGVFALLQWDFDLSTVAALLTILGYSVNDTVVVS
                     DRIRENLRKFKKMELNELLDRSINETLSRTILTGLTTLIVLVVLVLIGGEVIRYFSLA
                     MLLGVIIGTYSSMFIAAPLLGYTGLKRDWSSVAKGKKSPAVASGA"
     gene            complement(1667..3286)
                     /gene="secD"
                     /locus_tag="VF226_17210"
     CDS             complement(1667..3286)
                     /gene="secD"
                     /locus_tag="VF226_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7075779.1"
                     /translation="MLHFQRWKIIAITLTCLAGVLLALPNLFSKETLQSWPDWLPKRQ
                     LSLGLDLRGGAHLLLAMDVNDVRKDWLDTIRDDARARLREARIGVTGLGVQGGAVHVR
                     IAQQDQVGEALTKLKGLVQPIGNALLGSAGDDISVERGAEPNLIILQPTEAGMQQRIS
                     NAIAASIETVRRRIDTLGTSEASVVRQGADRILVQFPGLQDTTQLKALIGETARLSFH
                     EVHPSMSGEEALASRPPPGYKVYPSAEQDGFSYLLRETPVVRGDQLVDAQPGFDQRTN
                     QPIITFRFDQSGARTFGRFTQEHVGRPFAIVLDDKVLSAPVIQEPILGGSGQISGNFT
                     VESANNLAIQLRSGALPAKLTIVEERTVGPSLGADSIESGTRAFIVGGLATFVLITVV
                     YGTFGFYAGLALIINAILILACMTLLGAALTLPGIAGLVLTLGMAVDANVLIYERIRE
                     EIRAGKSAIAAIDAGFTRAIVTIADSQLTTLVAAIIMFWLGSGPIRGFAVTLSIGIFT
                     SVFTAVTVTRLLIALWVRSIRHKSRTVEVPV"
     gene            complement(3400..3732)
                     /gene="yajC"
                     /locus_tag="VF226_17215"
     CDS             complement(3400..3732)
                     /gene="yajC"
                     /locus_tag="VF226_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7075780.1"
                     /translation="MFITPAFAQGAPGGGSDFMLQLVPILLMFVIFYFLLFRPQQQRA
                     KQHREMVKNIRRGDTVITAGGIIGKVTRVKDETEIEVEIAENTRVRVVKATVADVKAK
                     GEPVKESA"
BASE COUNT          772 a         1221 c         1200 g          675 t
ORIGIN      
        1 gaggatggca gcaccgacgt gaatccatcg gaggaggaat gagaggaagc cgagcaaatc
       61 ggcgcccgcc agcgccagcc acaggatcag gatggcaagc gtgagcacgg cgccggccgc
      121 cagcgcacgc ccggcggttt cagtaatggc atccatttcg ggctcctgaa ggcgtgagaa
      181 tcgtgaggtc gccagaatgc cggaccatga cacattcccg acttcacgac ctcacgattt
      241 cacgatttca cgatttcacg tctcacggga taaaatcagt ccaccgccag caatgcggct
      301 gccacgcggc ggtgctcggc cagcaggacg ttgtaggtgc ggcaggctgc gcccgtatcc
      361 atgggctcga gcccgagccc agccgcgacg aacccattgc ggatttcacg ggaggggaag
      421 atttgcgtcc gtcccgtgcc gaggaggaga aagtcgatcc tctccttctc tgcgaacacc
      481 tgggcgaacg cgtcgaggct gaatgcatcg gcgcccgaga tgtcccaggc atagatgccg
      541 ctcggcaggc acaggatcga gcccctgtgg ctcatttcgg cgaaccgaaa gccaccgttg
      601 ccgtaagcat cgatgggggc ttgataagga tagcgtgcag gcttttcggc catggctgcc
      661 atcggatcag acacccccaa tcctcaggcc ccgctcgcga cggccggtga cttcttgccc
      721 ttcgcgacac tcgaccagtc gcgcttgagg cccgtgtagc cgagcagcgg cgccgcgatg
      781 aacatcgacg agtacgtgcc gatgatcacg ccgagcagca tcgccagcga gaagtatcgg
      841 atcacctcac cgccgatcag caccagcacc accagcacga taagcgtcgt caggcccgta
      901 aggatcgttc gtgaaagagt ctcgttgatc gaccggtcga gaagctcgtt cagctccatc
      961 tttttgaact tgcgcaaatt ctcccggatg cggtccgata cgaccacggt gtcgttgacc
     1021 gaatagccga gaatggtcag caacgctgcc acggtggaca ggtcgaagtc ccattgcagc
     1081 agcgcgaaca cgccggcaac caacagcacg tcgtgagcca gtgccagcac cgctccgacc
     1141 gcaaactgcc attcgaagcg gaaccagaca taggcagtaa ttccgataat ggagacgagg
     1201 acggcaatga tgcccgtcgt ccgaagctcg ctggaaaccg cagggccgac gatttcgaca
     1261 cggcgctgca cgaaatcatc ccccagcgcg gccaggacct tgttcacggc ctgaggctgc
     1321 gcctcttccc cacctggctg ctcctccacg cggatcagaa tgtcgtttgg cgcgccgaac
     1381 tcctgaatct gcacctcgcc aaggccgagc gatcccaatt tggatcgcac gctcgccaga
     1441 tcgacgggtc cgctcttgga ctggacctcg atcatggagc cgcccttgaa gtccacgccg
     1501 taattgaacc cgacggtcgc aatcagaacg atcgacaaga gcatcgcgat aaacgagccg
     1561 atcaggcata cgcccttgaa acgcatgaac ggaatgcgtg cgtcggctgg gaagagattg
     1621 atgcccttga aattcggaac cagaaacatg cgaggattcc tgtgtatcag accgggactt
     1681 cgaccgtacg gctcttgtgc cggatcgacc tcacccacag cgctatgagg agccgcgtta
     1741 ccgtgacggc cgtgaagacc gacgtgaaga tgccgatgct gagggtcact gcgaagccgc
     1801 ggatcggtcc ggacccgagc cagaacatga tgatcgccgc taccagcgtc gtgagctgcg
     1861 agtccgcgat ggtcacgatt gcacgcgtga acccggcgtc tatggcagcg atagccgatt
     1921 tcccggccct gatttcttcg cgaatgcgct cgtagatcag cacgttcgcg tcaaccgcca
     1981 tgccgagcgt cagcacgagg ccagcgatgc cagggagcgt caaggccgcc ccaagcaggg
     2041 tcatacaggc caagatcagg atggcgttga tgatgagggc aagaccggca tagaagccga
     2101 acgtcccgta gaccaccgtg atcaggacaa aggtcgcaag gccgcccacg atgaaggccc
     2161 tggtccccga ctcgatcgag tcggcgccga gcgaaggccc gacggtgcgc tcctcgacga
     2221 tcgtcagctt ggcgggcagc gcaccggagc ggagctggat tgcgagattg ttggcgctct
     2281 ccaccgtgaa gttgcccgag atctgacctg aaccgcccag aatgggctcc tggatcacgg
     2341 gcgccgacag caccttgtcg tcgagcacga tggcgaacgg acggcctacg tgctcctgcg
     2401 tgaagcggcc gaaagtccgc gcgccggatt ggtcgaagcg gaaggtgatg atgggctggt
     2461 tggtgcgctg gtcgaaaccg ggctgggcat cgaccagctg gtcgccgcgc acgacagggg
     2521 tctcgcgcag taggtaagaa aaaccgtcct gctcggccga ggggtagacc ttgtagccag
     2581 gcggcggccg cgaggcgagc gcctcctcgc ccgacatgct cggatggacc tcgtggaagc
     2641 tcaggcgcgc ggtctcaccg atgagagcct tgagctgtgt cgtgtcctgc aggccgggaa
     2701 actggaccag gatgcgatcg gctccttgcc ggacgactga ggcctcggat gtgcccaacg
     2761 tgtcgatacg ccggcgcacc gtctcgatgg atgcagcgat ggcgttcgag atccgctgct
     2821 gcatccctgc ctcggtgggc tgcagaatga tgaggttcgg ctccgcgcca cgctcaacgc
     2881 tgatgtcgtc accggcgctg ccgagcagcg cattcccgat cggctgaacg aggcccttca
     2941 acttcgtcaa cgcctcgccg acctgatctt gctgagcgat ccgcacatga accgccccgc
     3001 cctgcacccc gaggcccgtg accccgatgc gtgcttcgcg caggcgcgcc cgggcatcgt
     3061 cgcggatcgt atccagccag tccttgcgga cgtcgttcac gtccatggcc agaaggagat
     3121 gtgcgccgcc ccgcaggtcg agaccgagac tgagctgccg cttcggtagc caatcgggcc
     3181 aggattgcaa ggtctctttc gaaaacaggt tgggcagcgc gaggaggacg cccgcgaggc
     3241 acgtcagcgt gatggcgatg atcttccagc gctggaaatg gagcatctgg tctcaaactc
     3301 ctcggctcac gacgtatcgg gccgctgctg ggatggggct cggatatcgg cgagcctcct
     3361 ggtgtaatcg acgttgacgg gcagcccgaa cccaaacggt caggcgcttt ccttcaccgg
     3421 ctcgcccttg gccttgacat cggcgacagt cgccttgacc accctcaccc tggtgttctc
     3481 ggcgatttcg acctcgatct cggtctcgtc cttgacgcgc gtaaccttgc cgatgatgcc
     3541 gccagcagtg atcacggtat cgccgcgccg gatgttcttc accatctccc ggtgctgctt
     3601 cgcgcgctgc tgctggggcc tgaacaacaa gaagtagaag attacgaaca tcaataatat
     3661 gggtacgagc tgcagcatga agtcgctgcc gcccccggga gcgccttgcg cgaaggctgg
     3721 agtgatgaac atgtggcttt cgtccttgag ccggctcagg taacgacaat ccggccccgc
     3781 ggcactatta cctgcgggcc tgtccgtctg actgcggcgc gactatagtg gtccgtttcc
     3841 gcttggcaag tgatgcctcg cccgcggc
//
LOCUS       DASVDU010000265        17230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_245345, whole genome shotgun sequence.
ACCESSION   DASVDU010000265 DASVDU010000000
VERSION     DASVDU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17230
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245345"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1353
                     /locus_tag="VF226_17220"
     CDS             7..1353
                     /locus_tag="VF226_17220"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX7075781.1"
                     /translation="MRLSRYFLPVLRETPKEAEIISHQLMLRAGMIRQASAGIYSWLP
                     IGFRVLKKIEQIVREEQNRAGAIEVLMPTIQSAEIWRESGRYEAYGKEMLRIIDRHER
                     EMLYGPTNEELITQIFRDSVKSYKDLPRNLYHIQWKFRDEIRPRFGVMRGREFLMKDA
                     YSFDLTPEAGRHAYNRMFVAYLRTFARMGLQSIPMAAETGPIGGDLSHEFIILAETGE
                     SQVFCHKDLLEGRIPPADTDFDADLTPIVKAWTSLYAATDEKHDPDRFDREVPKDSQV
                     TARGIEVGHIFFFGTKYSEPMNCRVQGPDGKMVPLQMGSYGVGVSRLVGGIIEACHDD
                     AGIVWPVPVAPFEVGLINLKAGDVATDEACNRAYQQLKNAGIEVLYDDTDERPGVKFT
                     TMDLIGLPYQVIVGPKGLKGGEVELKVRKGGVRESLTPDAAVQRVIDLVRSQRVLA"
     gene            1463..2731
                     /locus_tag="VF226_17225"
     CDS             1463..2731
                     /locus_tag="VF226_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEX7075782.1"
                     /translation="MSHSVDTRPFAPYEWLLALRYLRARRKEGFISVIAGFSFVGILL
                     GVATLIIVMAVMNGFRNQLIEKILGINGHIVVYKIAEPFDDYENVRKRVATAVGVRGA
                     VPLVEGQVMVSSPTQALGAYVRGISEEGLQSLPLVWNNVLSGSLGGFDEQTGIAVGSR
                     LASALGVQLGDTLTLVSPRGSSTPFGTAPRTKPYRIAAIFEMGMAEYDRTMLFMPLQE
                     AQKYFSRGNQVDVLEVMINDPERVDGTIAELQRVLGDGYSMVDWRQRNQTFFTVLEVE
                     RTVMFIILSLIVLVAALNIISGLMMLVKDKGRDIAILRTMGATRGAMMRVFLITGASI
                     GVVGTVAGLIIGIIFCWNIESIRQFVQWLTGTTLMDPTVYYLSRLPADIDVGETASIV
                     AMALILSLLATLYPSWRASRLDPVEALRYE"
     gene            2749..3459
                     /locus_tag="VF226_17230"
     CDS             2749..3459
                     /locus_tag="VF226_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075783.1"
                     /translation="MQELTQRPVLQLVGLSRTYRQGNRAVQVLAGAYADLYPGEAVAL
                     VGPSGAGKSTLLHMAGLLETPDTGRVIVDGRDCSQMSDDERTRVRRGKMGFVYQFHQL
                     LPEFSALENVVIPQMICGRPRRKAEARARDLLVAMGLGHRLDHRPAELSGGEQQRTAI
                     CRALANSPALLLADEPTGNLDPSTAEQVFAELIELVVRTGVAALIATHNMELAARMHR
                     VLRLEGGVLVEVAPGTVA"
     gene            complement(3493..4473)
                     /locus_tag="VF226_17235"
     CDS             complement(3493..4473)
                     /locus_tag="VF226_17235"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7075784.1"
                     /translation="MGLLVDGVWHDQWYDTKSTGGRFVRQDSAFRNWITPDGSPGPSG
                     VGGYKAEAGRYHLYVSLACPWAHRTLIFRKLKGLEAMIGVSVVHWLMLENGWTFADGP
                     GVVPDPIFGARYLYEIYARAEPHYTGRVTVPMLWDKKTGTIVNNESSEIIRMFNSAFD
                     GCGAAPGDYYPEDLRPEINALNDRIYDTVNNGVYKAGFATTQEAYDSAFAELFATLDV
                     LDERLATRRYLLGNRLTEADWRLFTTLVRFDPVYFGHFKCNLRRIKDYPNLSGYLRDL
                     YQKPGIAETVNMAHIKGHYYQSHRTINPTGIVPRGPELDLNRPSGRDRLQ"
     gene            complement(4475..4597)
                     /locus_tag="VF226_17240"
     CDS             complement(4475..4597)
                     /locus_tag="VF226_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075785.1"
                     /translation="MIDLKSRLNEADPSVARERTIVKSGCIDQIRRKSSSQKGN"
     gene            complement(4782..6101)
                     /locus_tag="VF226_17245"
     CDS             complement(4782..6101)
                     /locus_tag="VF226_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7075786.1"
                     /translation="MMGAVLSPGQAALILFGLFFLLMFMRVPVAFALGLACLPILAIE
                     PRLDTMTLMQETFNAYNSFILLAVPFYLLTANIMNIGGTTDRLMKLSRSMVGHFPGSL
                     AQINVVLSMFFAGISGSATADAASQSKLFIEAQTREGYDLSFSVAITAVSAVLAVVIP
                     PSIVMIVWGGILTVSIGGLFLAGILPGLLIGLAQMATVHFYAKSRGYKTYTRSTWREF
                     FCAAWVSIPALMTPAIIIGGKIFGLFTATEAACIAVLYATVLSVVVYREMDAKGLYGA
                     LLDTGKLSGITLFCVGTASTFGWLLAYYQIPQAILSGVTAWEMGFIATGFFIAAVFLV
                     VGCFLDAIPAIIICAPILEPLTKMVGMEPIHFAMISIISLAFGLVTPPYGLCLLIACA
                     IAGIPMRDALKDTCIMLLPMLTVLGLVIVWPDVALMVPRILAPEFLR"
     gene            complement(6098..6619)
                     /locus_tag="VF226_17250"
     CDS             complement(6098..6619)
                     /locus_tag="VF226_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7075787.1"
                     /translation="MAFSTLPPDRRSLWRRFTAWYANFLSWLMAISVGVLVLPVSLQI
                     FSRYTSLIPAYIWTEEMARFLFIWTIMIGAMIGVRETSHFEVDVWPRMGPRGEAIARF
                     TGRIGVLATAIIFVTAGIKFTEFAMNRISELAELPLWYIHVAWPVTGLTWIVFLGEQM
                     FDDLRVIFGRASA"
     gene            complement(6726..7724)
                     /locus_tag="VF226_17255"
     CDS             complement(6726..7724)
                     /locus_tag="VF226_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7075788.1"
                     /translation="MGRRTVLGGLGATAVAGAIGMPAIVRAQDPITLNGASQFNDDHA
                     FTKTMAKFGELVTEAWGKPINFVLHKNRALGLEKQYFEYMSQGRAVDYAVVSPAHMST
                     FSKAAPFLDAPFLFRDLDHWNKVLDSDVLKPIADEIAEKADVMLIGYAGGGVRNIFVN
                     EPMKSLEDIQGLKIRVQGAPIWSRAFQAVGMSPTVIAYDEVYNAIQNNVIKAGENEAA
                     GVEQMKFYEVGPNLLLTQHAITIRPLCFSGKTFRNLPKDLQDIVLKAGKEAGKYGREV
                     ESSEDAKKLEALEKAGKLKRIPFEGREEMKKRVDPVMEAYAKEIGAEEIYKKIMAM"
     gene            7854..9002
                     /locus_tag="VF226_17260"
     CDS             7854..9002
                     /locus_tag="VF226_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX7075789.1"
                     /translation="MKIVEVRTHVLEAKIETPFSWSFNRADTRASCIVEIVCEDGTTG
                     WGECFGPARLNAAVVQAFRPLLLGQDALATERIWQFLYNHFRDQGQKGLIITALSGVD
                     IALWDIKGKRFGAPVHQLLGGPLRTSVAAYATGTYRRDTGDPLDYITEEVAGYVREGF
                     GAVKLKIGFGLEQDIALIRAVRETIGPRTGLMLDANHGYDAIDAIALGRAVAPLDIGW
                     FEEPVVPDDLGSYEEVRRGQPIPVAGGECEFTRWGFREVLVRRAMDIIQPDTCAAGGL
                     SECKKIADMANAFGLRYLPHVWGTGIGLAAALQLLAVLPHNPPRHTPREPMLEFDRSE
                     HPFRQAVLTSPIEHEAGVVKVPDGPGLGIEVDRSALERFAARECSQQE"
     gene            9451..10470
                     /locus_tag="VF226_17265"
     CDS             9451..10470
                     /locus_tag="VF226_17265"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7075790.1"
                     /translation="MKKSLFAALAFGLVAGVASSATAQTDITWGTSAVGSSGHRALVN
                     LATILNRELKDYQVTVQPMPGAIISVKSYATGQTLGYYGADIAFYELANDTARFKGFK
                     AQMKRQPVQSFWTYTVEVGVAIHERDKDKIKSWSDLTGKQVFTGPLPWDTRAQLERGL
                     AALGVKHNYVEVDLSSAGSLMDSGRLDGFIIYTNAESTTAPWITEAGLSTKWIALNPT
                     PEEIAKLKEKNFSVVEVKSEAFKHDTGTETVVLLPFFYGFHVGMEIPEEDVYKMLKVI
                     EANVDELVKLDAGFAQLKDMANMQVRGVTASIEYVPVHPGLAKWMKEKGVWNSAWDDR
                     IAKKN"
     gene            10555..12501
                     /locus_tag="VF226_17270"
     CDS             10555..12501
                     /locus_tag="VF226_17270"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7075791.1"
                     /translation="MSTTYPSQVRVALTALYYATAVFFSVYLFNYYFTGEGGPTVLTV
                     LLLPVCFILFVLESLRSNDFYPRLGLIPNLVIAAIYIGLALTVLYYVSTEFYDIRTVR
                     AGFWNTTDLVMGAIMVLLIMEYTRKRYFVLFILNVVLVLYAVYGWIVPGAFGHPGLSW
                     TRIISAMSLETATGVYSRLPQLALTLIGSFLLVLSVLRAFGCVDSILLGADRITAWSP
                     YALPQAAVLGSFGVATVSGSSAANAATTGSATIPAMIQSGMPRETAASIETASSLGGQ
                     LMPPIMGISAFLMADFLGRSYFDVVARGYAPAIIYFISVSVAVYLLSIHFRPSRNVKL
                     PGDRFDWGDAINIAAFAAVVFALIGLMGVIHMAPMIAALKVFMYSAPPILVIYLVRRW
                     RSEGRPGFRDVVAPLGRMVDIFASMTADLTLLLATLSIMTGAFVITGIPTKVGAILIE
                     AAGVNLAAMGIVAFLFATLIGTGLPPAPTYILTALVIAPPMIRYGVDPWVVHFFAFFL
                     AVWGELTPPTSVVAAVTAKIADASFMSTLMRALVLCVSLFMLMAGVFARPELVLEPGL
                     SQVGAMLLILTASIGMTFSLQAHFAEARTADLALRGGLGAMSLVVLLYPDDTVAALFG
                     IPVIAMIVGWYLKRHPSRIATPAA"
     gene            12947..13594
                     /locus_tag="VF226_17275"
     CDS             12947..13594
                     /locus_tag="VF226_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075792.1"
                     /translation="MSFADIREMVEINEANRDSWIPLLPIFDYRKFTATPDNIFCIVA
                     RDPHGRVVGTHACRLYDWSGTNFHEEAVSLRLFYDDPDKMRRPGESCRVTAPSAKSVG
                     GLTVFSGAAWYHPDYRGRGLSTILPHVSKAYALTRWPAETIVSIMAESVHQKGFAKRF
                     GYTNVDWEIFLENSVVGTMRVAFLSVKREETFSILAEHLAGSSQIDAGILRRHAQ"
     gene            complement(13541..14152)
                     /locus_tag="VF226_17280"
     CDS             complement(13541..14152)
                     /locus_tag="VF226_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075793.1"
                     /translation="MNSYANGTWHPQIMGAAASHSYASLVAILASSPSPGLRENRVLA
                     RFIPLSRLPKNSPLWRATKLWQDSGGDWRKLAVVSGLGAAVAHRYTLFEVHGKRNFVL
                     QGYGSGLPKCAQSWMVKAMGSPIHDHPDQSYIGDCVSSYTKVVESGQPALQDVDAYVS
                     WPDTEPQRRRYKRLVLPLSDDNRLVYLLSVTAEDTSINLRATG"
     gene            complement(14835..15665)
                     /locus_tag="VF226_17285"
     CDS             complement(14835..15665)
                     /locus_tag="VF226_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7075794.1"
                     /translation="MDAFICTACGTQYPPSHTPPASCPICEDERQFIPQTGQSWTTQE
                     KLALSHVNGWLEYEPDILGIGTQPRFAIGQRALLLRTGHGNVLWDCISMLDAATVTLL
                     KALGGIQAMAISHPHFYTSMTDWSRAFSVPVHVHADDREWIMRSDGELALWEGETLEL
                     LPDVTLIRAGGHFAGSSMLHWARGAGGRGVLCASDTAYVVADRKHVSFMRSYPNLIPL
                     GAREVNAIAEALAPFEFDMMFGNFFDSVIRTGAKQAVERSVRRYLSAIGSADPGDPAG
                     "
     gene            complement(15734..16990)
                     /locus_tag="VF226_17290"
     CDS             complement(15734..16990)
                     /locus_tag="VF226_17290"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7075795.1"
                     /translation="MESNSRAIFIGRRFERGTLGDPVSLDADLLPRHIAIFAGSGSGK
                     TVLLRRIVEEAALLGIPSIVLDINNDLVRLGDRWPTPPEGFGDDDVAKAAAYSERANV
                     VVWTPGVSTGNPISLNLLPDFAAIGDSRDEQTEQERFEAIEMARATLSPYLGGTGQKA
                     SLKRGILADALRAFAYRGGGSLDDLVELLSDLPEDVSKISDAPRLANQIADQILAVVA
                     TNPLLQSIGPCLDPQRLFYGSNGKTRVSVINLAGLASDEARQTFVNQLQMALFTWIKQ
                     NPSPSGRLYVLDEAQNFAPSQKVTPCKGSTLSLVAQARKYGLGMIFATQVPKGIDNAI
                     VSNCTTHVYGRMSSPATIQATQELMSAKGGAADDIGRLTRGEFYFSTDSFNRPVKVRT
                     PMCLSWHPANPPTAQEVIQKARSMRP"
BASE COUNT         3278 a         5201 c         5350 g         3401 t
ORIGIN      
        1 gtccacatgc gcctctcccg ctacttcctc cccgtgctcc gcgagacgcc caaggaagcg
       61 gagatcatct cgcatcagct catgttgcgc gccggaatga tccggcaggc aagcgccggc
      121 atctactcct ggttgccgat cggcttccgg gtgctgaaga agatcgagca gatcgttcgg
      181 gaggagcaga accgcgccgg cgccatcgag gtcttgatgc cgacgatcca gtcggccgag
      241 atctggcgcg agtcggggcg ctacgaggct tacggcaagg agatgctgcg catcatcgac
      301 cgccacgagc gcgagatgct ctacggtccg acgaacgagg agctcatcac ccagatcttc
      361 cgcgacagcg tgaagagcta caaggacctg ccgcggaacc tctaccacat ccagtggaag
      421 ttccgggacg agatcaggcc gcggtttgga gtcatgcgcg gccgcgagtt cctgatgaag
      481 gacgcttact ccttcgacct gaccccggag gcgggccgcc acgcctacaa ccggatgttt
      541 gtcgcctatc tgcggacctt cgcgcgcatg ggcctgcagt cgattcccat ggcggccgag
      601 acggggccca tcggcggcga tctgagccac gagttcatca tcctggccga aacgggcgaa
      661 agccaggtgt tctgccacaa ggacctgctc gaaggcagga ttccgcctgc agacacggac
      721 ttcgacgcgg acctgacccc gatcgtcaag gcatggactt cgctttacgc tgccacggac
      781 gaaaagcatg atccggatcg tttcgatcgt gaggtcccca aggacagcca agtgactgcg
      841 cgcggaatcg aggtcgggca catcttcttt ttcggcacga aatattccga gcctatgaac
      901 tgccgcgtgc agggtccgga cggcaaaatg gtgccgctgc agatgggctc gtacggcgtt
      961 ggcgtgtcgc gactggtcgg cggcatcatc gaggcttgtc acgacgatgc cggaatcgtc
     1021 tggcctgtac ccgttgcccc gttcgaggtt gggctcatca acctgaaggc tggtgatgtt
     1081 gcgacggacg aggcctgcaa tagggcctac cagcaactca aaaacgccgg aatcgaggtc
     1141 ctttacgacg acacggacga gcggccggga gtgaagttca caaccatgga tctgatcggg
     1201 ctgccctacc aggtgattgt cggcccgaag gggctgaaag gcggtgaggt cgagctcaag
     1261 gttcgcaaag gcggtgtgcg agagagcctg acaccggatg ccgccgtcca gcgcgtcatc
     1321 gacttggttc gcagccagcg tgtgctggcc tgagccggag agcgttcgtg caagcccggc
     1381 ccgcctcctt ccgccaccgc ccggtaccgg cgatgggtca acgggtgcgg tcagagatcg
     1441 gggcgaaagg aacggcgaaa gcatgagcca ttcggtcgac acacggccct tcgcacctta
     1501 cgaatggctg ctggcgctgc gctatctgcg ggcccggcgc aaggaaggat tcatatccgt
     1561 catcgccggc ttttcgttcg tgggcattct gctcggggtc gcgacgctga tcatcgtcat
     1621 ggccgtgatg aacggcttcc gtaatcagct catcgagaag atcctcggca tcaacggtca
     1681 catcgtcgtc tacaagatcg cggagccgtt cgacgactac gagaacgtac gcaagcgcgt
     1741 cgccacggcc gttggcgtcc gcggggcggt gccactggtc gaagggcagg tgatggtctc
     1801 gtcaccgacg caggctctcg gagcctacgt gcgtggaatc tcggaagagg ggctccagtc
     1861 gctgccgctc gtctggaaca acgtcctctc gggcagcctg ggcggcttcg acgaacagac
     1921 gggtatcgcc gtcggcagcc ggctcgcgag cgcgctcggc gtgcagctcg gcgatactct
     1981 gacgctcgtc tcgccgcggg ggagctcgac accgttcggc accgcgccgc ggaccaagcc
     2041 ttaccgcatc gccgccatat tcgagatggg catggccgag tacgaccgca ccatgctgtt
     2101 catgccgctc caagaggcgc agaaatactt ctcgcgcggc aaccaggtcg atgttctcga
     2161 agtaatgatc aacgatcccg agagggtcga tgggaccatc gcggagctcc aacgcgtcct
     2221 cggtgacggc tactcgatgg tggactggcg gcaacgcaac cagaccttct tcaccgtgct
     2281 cgaggtggag cgtacggtga tgttcatcat tctgtcgctc atcgtgctgg tcgccgcgct
     2341 caacatcatc tcaggtctga tgatgctggt gaaggacaag gggcgcgaca tcgccatcct
     2401 gcggacgatg ggagccaccc gtggcgcgat gatgcgggtc tttctgatca ccggggcttc
     2461 cataggtgtc gtcgggaccg ttgccggtct gatcataggg atcatcttct gctggaacat
     2521 cgaatccatt cgccagttcg tgcagtggct caccggcacg acgctgatgg acccgacggt
     2581 ctattatctc tcccgtcttc cggccgatat cgatgtcggc gagacggcat ccatcgtcgc
     2641 catggctttg atattgtcgc tgctggcgac gctctatcca tcctggcggg cttcgcggct
     2701 cgatcccgtc gaggcgctca ggtatgaatg aatgaccggg gggcgggggt gcaggagctg
     2761 actcaaaggc ccgtgctgca gctcgtgggg ctgtcacgca cctatcggca gggcaacagg
     2821 gccgtgcagg tgctggccgg agcctacgcc gatctgtacc cgggcgaggc ggtcgctctt
     2881 gtcggcccct cgggcgcggg caagtcgacg ctactgcaca tggcggggct gctcgagacg
     2941 ccggacacgg gccgggtcat cgtggatggc cgggactgct ctcaaatgtc ggacgacgaa
     3001 cgcacgcgcg ttcgtcgtgg caaaatgggc ttcgtctatc agttccatca acttcttccg
     3061 gagttctccg cgttggagaa cgtcgtcatt ccgcaaatga tctgcggccg gccgcgccgt
     3121 aaggcggaag cccgtgcacg ggatctgctg gtcgcaatgg gccttggcca ccggctcgac
     3181 catcgtccgg cggagctgtc cggcggcgag cagcagcgga cggcgatctg ccgtgcactg
     3241 gccaattcgc ccgcgctgct ccttgccgac gagccgaccg gcaatttgga ccccagcacc
     3301 gctgagcagg tcttcgccga gctgatcgag ctcgtggtcc gcaccggagt cgccgctctc
     3361 atcgccactc acaacatgga gctggcggcc cgcatgcatc gtgtgctgcg gctcgaaggc
     3421 ggcgttctcg tcgaggtcgc gccggggacg gtggcctaga ctggcgcgtc tccgcgctca
     3481 gtccgatcgc gcttattgca atctgtctcg tcccgacggc cggttgaggt ccagctcggg
     3541 ccctcggggg acgatgccgg ttggatttat cgtgcggtga ctttggtagt agtgaccctt
     3601 gatgtgggcc atgttcacgg tctcagcaat ccccggcttc tggtagagat cccgcaggta
     3661 gcccgaaaga tttggataat ccttgatccg cctcaggttg catttgaagt ggccgaaata
     3721 gaccggatcg aaacgcacca gcgtggtgaa caaccgccag tctgcttcgg tcagacgatt
     3781 tccgaggaga tagcggcgcg tcgccaggcg ctcgtcgaga acgtcgaggg ttgcgaagag
     3841 ctcggcgaaa gccgagtcgt aggcttcctg ggtagtggcg aaccccgctt tgtagacgcc
     3901 gttgttcacc gtatcgtaaa tgcggtcgtt caaggcattg atttccggcc gtaggtcttc
     3961 cggatagtag tcgcccggcg ctgccccgca tccgtcgaag gccgagttga acatccgaat
     4021 gatttcggac gactcgttgt tcacgatcgt cccggtcttc ttgtcccaca gcatcgggac
     4081 cgtcacccgc cccgtgtagt ggggctcggc ccgtgcgtag atctcgtaca ggtagcgggc
     4141 gccgaagatt gggtccggca cgacgcccgg cccgtccgcg aaggtccagc cgttctccag
     4201 catcagccaa tggacgacag agacgccgat catagcctcg agccctttga gcttgcggaa
     4261 gatcagtgtc cgatgtgccc agggacacgc cagcgaaaca tagaggtgat agcgtccggc
     4321 ctcggctttg tagccaccga caccgctcgg tccgggagag ccatcgggcg tgatccagtt
     4381 cctgaaggct gagtcctgcc gcacgaagcg accgcctgtg cttttggtgt cgtaccactg
     4441 gtcgtgccag accccatcga cgagaagtcc catttcaatt ccctttctgc gagctggatt
     4501 tgcgtcgaat ctggtcgatg cagcccgact tcacgatggt tcgttcgcgc gctacgctgg
     4561 gatcggcctc gttcaagcgc gacttcagat cgatcacgaa atgatcgcct ctccccttgg
     4621 gagatggtgg gagggagcgc gatccgccat gccgcgaagg gacgcaagcc gctgcccccg
     4681 ccctcgcaag tgggagggga gcagatgcgg tgtcggcgcg ttgcccttcg cccggtgcag
     4741 tggccttgtt cagaggatga tgcgagccgt cgaggcgcgc atcaccgcag gaactccggc
     4801 gcgaggatgc gcggaaccat cagtgcgacg tcgggccaga cgatgaccag ccccagcaca
     4861 gtcagcatgg gtagcaacat gatgcaggta tccttcaggg catctcgcat gggaatgccg
     4921 gcaatggcgc acgcgatgag caggcagagg ccgtagggtg gcgtcaccag accgaaggcc
     4981 aacgatatga tgctgatcat cgcgaagtgg ataggttcca tgcccaccat tttcgtcagc
     5041 ggttccagga tcggtgcgca aatgatgata gccgggatgg catcgaggaa gcagccgacg
     5101 acaagaaaca cggcagcgat gaagaaaccc gtcgcaatga agcccatttc ccaggcggtc
     5161 acgcccgaca ggattgcctg cgggatctgg tagtaggcga gaagccagcc gaacgtgctt
     5221 gcggtgccca cgcagaatag cgtgatgccg gagagcttac ccgtatcgag aagtgctccg
     5281 taaaggccct tggcatccat ctcgcgatag acgaccacgg acagaaccgt cgcatagagc
     5341 acggcaatgc aggcggcctc cgtggcggtg aacagcccga agatctttcc gccgatgatg
     5401 atggcaggcg tcatcagcgc ggggatcgaa acccatgcgg cgcagaagaa ctcacgccag
     5461 gtcgagcgcg tgtaggtctt gtatccgcgt gacttcgcgt agaaatgcac ggtcgccatt
     5521 tgtgccaggc cgatgaggag gcccggcaga atgcctgcca ggaacaggcc gccgatcgac
     5581 accgtgagaa tcccgcccca gacgatcatg acaatcgaag gcggaatgac gacggcgaga
     5641 acggccgaga ccgccgtgat cgcgaccgag aaggacaagt cgtagccttc gcgcgtctgg
     5701 gcctcgatga acagcttgga ctggctcgcg gcatcagcgg tcgccgagcc ggaaatgccg
     5761 gcgaagaaca tcgacagcac gacgttgatc tgggccaggc tgcccgggaa gtgcccgacc
     5821 atggagcgtg acagcttcat cagccggtcg gtggttccgc cgatgttcat gatgttggcg
     5881 gtgagcaggt agaagggcac cgctagcagg atgaaggagt tgtaggcgtt gaacgtctcc
     5941 tgcatcagcg tcatcgtgtc gagccttggc tcgatcgcca ggatcggcag acatgcgagc
     6001 ccgagcgcga aggcgaccgg cacgcgcatg aacatcagca ggaaaaacag cccgaacagg
     6061 atgagtgccg cttgacccgg cgagaggacg gcgcccatca tgccgacgcc ctcccgaaaa
     6121 tgacgcgcag gtcatcgaac atctgctcgc cgaggaagac gatccaggtg aggcccgtga
     6181 ccggccaggc cacgtggatg taccacagcg gcagctcggc cagctccgag atgcggttca
     6241 tcgcgaactc ggtgaacttt attccggctg tgacaaagat gatggccgtc gcgagcacgc
     6301 cgatccggcc agtgaaacgg gcgatggctt cgccgcgcgg ccccatgcgc ggccagacgt
     6361 ccacctcgaa atgagatgtc tcccgcacac cgatcatggc gccgatcatg atcgtccaga
     6421 tgaagaggaa ccgcgccatt tcctccgtcc agatgtaggc cggaatgagc gaggtgtagc
     6481 gggagaagat ctgcagactg accggaagga ccagtacgcc gaccgaaatg gccatcagcc
     6541 aggagaggaa gttggcgtac caggccgtga agcgtcgcca gagagaacgg cggtccggcg
     6601 gaagcgtcga aaatgccatt gggtcatcac cgggatgtgc gggaacagga cggccggggt
     6661 atgcccccgg ccggaaggct gcacagtgac ggcgcgagat ggccccgcgc cgttcacctc
     6721 atccgctaca tggccatgat cttcttgtag atctcctcgg ccccgatttc cttggcgtag
     6781 gcctccatca ccggatcgac gcgcttcttc atctcctcgc gcccttcgaa ggggatgcgc
     6841 ttcagcttgc cggccttctc cagcgcctcg agcttcttgg cgtcctccga cgactcgacc
     6901 tcccggccat acttacctgc ttccttgccc gccttgagca cgatatcctg caggtccttc
     6961 ggcaggttcc ggaaggtttt tcccgagaag cagagcggcc ggatggtgat ggcgtgctgg
     7021 gtcagcagca ggttcggccc cacctcgtag aacttcatct gctcaacgcc cgcggcctcg
     7081 ttctcgccgg ccttgatgac gttgttctga atggcgttgt agacctcgtc gtaggcgatc
     7141 acggtcggcg acatgccaac tgcctggaac gcccgcgacc agatcggagc gccctgcacg
     7201 cgaatcttga gaccctggat gtcctcgagc gacttcatcg gctcgttgac aaagatgttg
     7261 cgcacgccgc cgcccgcgta gccgatgagc atgacgtcgg ccttctcggc gatctcgtcg
     7321 gcgatgggct tcaggacgtc ggaatcgagc accttgttcc agtggtcgag atcacggaac
     7381 aggaagggcg catcgaggaa gggggctgcc ttggagaagg tcgacatatg cgccggcgag
     7441 accaccgcat aatcgaccgc ccggccctgc gacatgtact cgaagtactg cttctcgaga
     7501 cccagagccc ggttcttgtg gaggacgaag ttgatcggct tgccccaggc ctcggttacc
     7561 agctcgccga acttggccat ggtcttcgtg aaagcatggt cgtcgttgaa ctgcgatgcg
     7621 ccgttgagcg tgatcgggtc ctgtgcgcgc acgatcgcgg gcatgccgat cgcgccggca
     7681 accgccgtcg cacccaatcc tccaagaacc gttctgcgtc caatcattcg cgtctcctcc
     7741 agttttggcc ccacgccgta tagtggggcg gtacgtccac ccggccgctc gcggccaaag
     7801 tcggggcacg ataacttagg tcattccgca gcactagccc tctggagcga ttgatgaaga
     7861 ttgtcgaggt gagaacccat gtgctcgagg cgaagatcga gactccgttc tcatggtcct
     7921 tcaatcgcgc cgatacacgc gccagttgca tcgtcgagat cgtatgcgag gacggcacga
     7981 ccggctgggg cgaatgcttc gggccggccc ggctcaatgc ggccgtcgtg caggccttcc
     8041 ggccgttgct gctgggccag gatgccctcg ccacggagcg gatctggcag ttcctctaca
     8101 accattttcg cgatcagggg cagaagggac tgatcatcac ggcgctgagc ggcgtcgaca
     8161 ttgccttgtg ggacatcaag ggaaagcggt tcggagcgcc ggtccaccag ctcctgggcg
     8221 gccccctgcg gaccagcgtg gcagcatatg cgacgggcac ctatcgccgc gacacgggcg
     8281 acccgctcga ctacatcacg gaggaggtgg cgggctacgt gcgtgaaggc ttcggtgcgg
     8341 taaagctcaa gatcgggttc gggctcgagc aggacatcgc gctcattcgg gcggtgcgcg
     8401 agaccatcgg cccgcggacc gggctgatgc tcgatgccaa tcacggctat gatgccatcg
     8461 acgccattgc ccttggccgc gccgtggcac cgctcgacat cggctggttc gaggagccgg
     8521 tggtcccgga cgacctcggg tcctatgagg aggtgcgccg tggccagccg atcccggtgg
     8581 caggcggcga gtgcgagttc acccgctggg gcttccgcga ggtgctggtg cggcgggcga
     8641 tggacatcat ccagccggac acctgcgccg ccggcgggct ttctgaatgc aagaagatcg
     8701 ccgacatggc gaatgcgttc ggcctccgct acctgccgca cgtctgggga acgggaatcg
     8761 ggctcgccgc tgcgctgcag ctcctggcgg tgctgccgca caatccgccc cgccatactc
     8821 cgcgcgagcc catgctcgag ttcgaccgct cggagcaccc tttccgacag gccgtgctga
     8881 catcccccat cgagcacgag gctggtgtcg tcaaggttcc ggacgggcca gggctcggaa
     8941 tcgaggtcga ccgttctgcg ctggaacggt ttgcagcgcg agagtgctct cagcaagagt
     9001 gaaagagtaa ataagcgtgc tgcccgcgtc aggtcgggca gcacgccggc tgtggagtaa
     9061 atgaagcgat ggcagccccg tttgggccga ttgcctgccc cccagccgcc atcgcccgcc
     9121 ctcggctgca acgctgggca catgcagcct gcggacggtt cggatcgtcg tacgccgggg
     9181 cacttgcagc aaccaacctg cggttgatct gccgattatg ctttccaggt tgtgagcgaa
     9241 cttggagatt gcacgttgca ggcacaggcg acgcgcgatg cagatcccgc tagggattgt
     9301 ttcggaattt tgacccgatt tggctttgcc actcggacat tcctcattaa gattttctta
     9361 gtccagaagg acaccagaag tgcgcgggcc ggtggcggca tgtcgcggct ggttcagcgc
     9421 aacccaaact ttgctaggga ggtgctggaa atgaaaaaga gcttgttcgc cgctcttgcc
     9481 ttcgggcttg ttgccggtgt ggcgtcatca gcaacagcgc agacggacat cacgtggggg
     9541 acttccgcgg tcggctcttc cggccaccgt gcacttgtca atctcgcgac cattctgaat
     9601 cgcgagctga aagactatca agtgaccgtt cagcccatgc cgggcgcgat catctcggtg
     9661 aagagctacg ccaccggtca gacgctcggc tactatggcg cggacatcgc gttctatgag
     9721 ctcgccaacg acacggcgcg gttcaagggc ttcaaggcgc agatgaaacg gcagcccgtg
     9781 cagtccttct ggacttacac agtcgaggtc ggtgtggcca ttcacgaacg tgacaaggac
     9841 aaaataaaaa gctggagcga tctgacggga aagcaggtct tcaccggccc actgccatgg
     9901 gacacgcgcg cccagctcga gcgcgggctg gcagcactcg gtgtcaagca taattatgtc
     9961 gaggtcgatc tctcgagcgc tggctcgctg atggattcgg gccgtctcga cggtttcatc
    10021 atctacacga atgccgagtc gacgaccgcg ccctggatca ccgaggcagg gctgtctacc
    10081 aagtggatcg cgctcaaccc gacgccggag gaaattgcga agctgaagga aaagaacttc
    10141 agcgtcgtcg aggtcaagtc agaggccttc aagcacgata ccggcaccga gacagtggtg
    10201 cttctcccgt tcttctacgg cttccatgtc ggaatggaga ttccggagga agacgtttac
    10261 aagatgctca aagtcatcga ggccaatgtc gacgagctcg tgaagctcga tgccggcttt
    10321 gcgcagctca aggacatggc caacatgcag gttcgtggcg tcaccgcctc gatcgagtac
    10381 gtgcctgtgc atccgggcct cgcgaagtgg atgaaggaga agggcgtgtg gaactccgcg
    10441 tgggacgacc gcattgccaa gaagaactga ttccggtcac ggcaaggcag tgaaaggcgg
    10501 cgcacccatg cgcctgcctt tcttcttttc agcacttaaa ccggctcgat ccttatgtcc
    10561 acgacctacc cttcgcaggt gcgcgtcgcc ctaaccgcgc tctactacgc tacggctgtc
    10621 ttcttttccg tctatctgtt caactactac ttcactggcg aaggcgggcc gaccgtgctg
    10681 acggtgctgc tgctgccggt ctgcttcatt ctttttgttc tcgaatcctt gcgcagcaat
    10741 gatttctatc cacgattggg gctgataccg aacctcgtga tcgcggccat ctatatcggg
    10801 ctggctctga cggttctcta ctacgtaagc accgaattct acgacattcg taccgtccgc
    10861 gccggcttct ggaacacgac cgacctcgtg atgggcgcca tcatggtcct gctcatcatg
    10921 gagtacacgc gcaaacgcta tttcgttctc ttcattctga atgtcgtcct ggttctatac
    10981 gccgtctatg gatggattgt gccgggcgcg ttcggtcatc cgggcctgag ctggacgcgg
    11041 atcatctcgg caatgagctt ggagacggca acgggcgtct attcacgcct gccgcaactc
    11101 gcactgacgc tgatcggatc ttttctgctg gttctaagcg ttctgcgcgc atttggttgc
    11161 gtcgattcca ttctcctcgg cgccgatcgc attaccgctt ggtcacccta tgctttgccc
    11221 caagcggcgg tcctcggctc cttcggcgtc gcaaccgtca gcggatcgag tgctgccaat
    11281 gccgcaacga ccggatccgc gacaattccg gcgatgatac aatccggaat gccgcgcgag
    11341 acggcggcat cgatagagac tgcctcatca cttggcggtc agctcatgcc accgattatg
    11401 ggcatctccg catttctcat ggcggacttt ctgggtcgga gctatttcga cgtggtggcc
    11461 cgcggttacg caccagctat aatctatttc atttctgttt ccgtggcggt ctatctgctg
    11521 tccatccact ttcgaccaag ccggaatgta aagcttccag gcgaccggtt cgattgggga
    11581 gacgcaatca acattgcggc gtttgcggcg gtggtattcg cgttgatcgg cttgatgggc
    11641 gtcatccaca tggcacccat gatagcggcg cttaaagttt tcatgtactc ggcaccgcca
    11701 atcctggtga tctacctcgt ccgccgctgg cgctccgagg gccgccccgg gttccgggat
    11761 gtcgtcgccc cgctcgggcg aatggtggac atcttcgctt ccatgaccgc cgacctgacg
    11821 ctgctgctgg caacgctctc catcatgacc ggcgctttcg tcattaccgg cattccgaca
    11881 aaggtcggcg ccatactgat tgaagctgcg ggcgtcaatc tggctgccat gggcatcgtc
    11941 gcattcctgt ttgcgacgct gatcggtacc ggcctgccgc ctgcgccgac ctacattctg
    12001 accgcactgg tgatcgcgcc accgatgatc agatacggcg tcgatccctg ggtcgtgcac
    12061 ttcttcgcgt ttttcctcgc cgtctggggc gagctcaccc cgccgacttc ggtggttgca
    12121 gcggtcaccg ccaagattgc cgatgcctcg ttcatgagca cactcatgcg ggcgctggtg
    12181 ctctgcgtgt cgttgttcat gctcatggca ggcgtgttcg cgcgtccgga gctggtgttg
    12241 gaaccgggac tatcccaggt tggcgccatg cttctgatcc tgacggcgag cataggcatg
    12301 acgttcagct tgcaggccca tttcgccgaa gctcgcaccg ccgatctggc attgcgcggc
    12361 gggctaggcg cgatgtcgct ggtggtcctg ctgtacccgg atgacacggt cgctgcgctg
    12421 ttcggcatcc ccgtcatcgc catgatagtc ggctggtatc tcaaacgcca tccaagccgg
    12481 atagcgacac ccgcagcctg aatggccaac aggcgtcgat ctgcggcgaa gaatgcatcg
    12541 caaaagtgat tcattctgtg cgcttgcaga atgaatccgt tccaaaatgg aacacccgtt
    12601 aagaattgat ttacattcgc tgcggcatga aagcttgcct gtcccaaatt gaaacagtca
    12661 gtaacacgaa tattgctctg tttcgtcata tgtgagacga tcatcaacga acatcgccgc
    12721 gccggcatct cgctggggag ctgctcgaat ccagtgccgg tttcggcggc aacagacgtc
    12781 gggggcaata tctatgaagc cgcagtctgc gagatcggct ccgctaccgc cagcagatca
    12841 aactggtgat ttcctcagtc gactggaggt ccgctatggc ccggtaggat tgctgggccg
    12901 cttcttcctg aaagcggatt cggaggcacg agcgcgaggg gtgtccttga gcttcgcgga
    12961 tatccgggag atggtcgaaa tcaatgaagc caaccgggac agctggatcc cgctgcttcc
    13021 gattttcgac taccgtaaat tcactgcaac tccggacaac atattctgca ttgtcgcgcg
    13081 cgatccgcac ggccgcgtcg tgggcacgca cgcctgtcgg ctttacgact ggtccggcac
    13141 gaatttccac gaagaggcgg tcagcctccg cttgttctac gatgatccgg acaaaatgcg
    13201 gcggcccggt gagtcctgtc gggtgacagc gccttcggca aagagtgtcg gcgggttgac
    13261 ggtattctcc ggtgcggctt ggtatcaccc cgattatcga gggcgcggac tatcgacgat
    13321 cctgccgcac gtgagcaagg cctatgcgct gacgcgctgg ccggcggaga cgatcgtcag
    13381 cataatggcg gaaagcgtgc accagaaagg cttcgccaag cgtttcggat acacgaatgt
    13441 agactgggag atctttctcg agaactccgt ggtgggcacc atgcgggtcg ccttcctatc
    13501 cgtcaagcgg gaagagacgt tcagcattct cgctgagcat ctagccggta gctcgcagat
    13561 tgatgctggt atcctccgcc gtcacgctca gtaggtagac gaggcggttg tcatccgaca
    13621 gcggtagcac gagacgctta tagcgccgcc gctgcggctc agtgtccggc cagctcacat
    13681 aggcatcaac gtcctgcagc gcgggctggc cgctttcgac gaccttggtg tagctgctga
    13741 cgcaatcgcc gatgtagctc tggtcaggat ggtcgtggat cggcgagccc attgccttca
    13801 ccatccagct ttgagcgcat ttcggcagcc cgctgccgta cccttgcagc acgaaattac
    13861 gtttgccatg gacctcgaac agtgtgtagc gatgcgcgac ggccgctccc aaccccgaca
    13921 cgaccgcaag cttgcgccaa tctccgccgg agtcctgcca gagcttcgtg gcgcgccaaa
    13981 gtggtgaatt cttcggcagc cgtgacaggg gaatgaagcg cgcgagaacg cgattttcac
    14041 gcaatcccgg tgacggactg gatgcaagaa tggcgaccag cgaagcgtac gagtgcgagg
    14101 ccgctgcccc catgatctgc ggatgccagg tgccgttcgc ataagagttc accagaaccc
    14161 gttcgattct ttcgcttgaa agccagtcga cagcttttgt cagggcggat ggcgcgaccg
    14221 tttcggggcg aagccacacg gtggcggatt gcccccgccg atcgatccgg acgaaatccc
    14281 atattcttca cgacgtaatc aggaaagttt cggactccga agccgtcgat ctcaaatctc
    14341 aattggctcg aatcactgct ccataggttt ccgtcatcgt caacaagaat agacgggttt
    14401 gtcatttctg cccgcagaat gtacctgcgc ccccagcagc tcgtgtacag tctagttggt
    14461 ttggagcata gcggcgccgt aatggcacgc aagatgtaaa ccactgaatg ggcggcgaaa
    14521 ttttgccatc cttagtttaa ggtgagcgag gaattgcgcg acctgaggaa atgcgagcag
    14581 ttctcgtaaa ggatcattaa ggagtcactg ctcgggcgtg catccgattg cgagcgatct
    14641 ggatatagca accgctgacc agttagggtc aacttggggg gcggcaaagt ttggaaacca
    14701 gtcgccgatt tggcgcgcat gtaattgccg tgccaagcca attcgacgga ctgacgcaac
    14761 agttaagatt gtgtttcgtg caggcccaaa ggcgccgtcg atgccgcctg gtcgctggtt
    14821 tagcaggtgg cggcctaacc cgctggatcg cctggatccg ccgacccaat tgcagacagg
    14881 tatcgcctga ccgacctctc gaccgcctgc ttcgcgcccg tgcggatcac gctgtcgaag
    14941 aagttgccaa acatcatgtc gaactcgaat ggcgcaagcg cttcggcaat cgcgttcacc
    15001 tcccgtgcgc caagcgggat caggttcgga tagctgcgca tgaaggacac gtgttttcga
    15061 tcggcgacca cataggcagt gtccgaagcg cacagaaccc cacgtccgcc agcgcctctt
    15121 gcccagtgca gcatgctgga accggcgaaa tgcccgccgg ctctgatcaa ggtcacgtcg
    15181 ggaagcaact ccaatgtctc gccctcccac agcgccagct caccatccga gcgcatgatc
    15241 cattcgcggt catccgcatg gacatggacg ggaacgctga aggcgcggct ccagtcggtc
    15301 atgctcgtgt agaagtgcgg atgtgaaatg gccatcgctt ggatgccacc cagcgccttg
    15361 agaagcgtca ccgtggcggc atcgagcatc gaaatgcaat cccacagaac gttgccgtgg
    15421 ccggtccgca ggagaagcgc ccgttgcccg atggcgaaac gcggttgcgt tccgatgccg
    15481 agaatatccg gctcgtactc gagccagccg ttcacatggc tgagagcgag cttttcttgc
    15541 gtagtccagg attgtccggt ttgcgggatg aactgtcttt cgtcctcgca gatcgggcag
    15601 cttgccggcg gagtatgcga tggtggatat tgcgtaccgc aagcagtgca gatgaaagcg
    15661 tccatcgaag gctcctccat cacagctggc gctcactgcc attcttaacg ctgcgcgatg
    15721 gcaattgcac ccactagggc cgcatggatc gggccttctg aatgacttcc tgggcagtcg
    15781 gcgggtttgc tgggtgccag ctcagacaca tcggcgtgcg aactttcacc ggccggttga
    15841 agctgtccgt cgaaaagtag aactcacccc tggtcaatcg accgatgtca tccgccgctc
    15901 cacccttagc cgacatcagc tcctgcgtcg cctgaatcgt cgcaggagag ctcatccgcc
    15961 cgtagacatg ggtcgtacag ttcgaaacta ttgcattatc tatgcccttg ggaacttgtg
    16021 tcgcgaaaat cattccaagg ccgtatttgc gcgcctgcgc gacaagcgac agggtgctgc
    16081 ccttgcatgg ggtgactttc tgcgaaggcg cgaaattctg cgcctcgtcg agcacgtaaa
    16141 gacggccgga cgggctggga ttctgcttga tccaggtgaa cagcgccatc tgcagctggt
    16201 tgacgaacgt ttgccgggcc tcgtcgctcg ctagccccgc aaggttgatg acggaaacac
    16261 gcgtcttgcc gttgctgccg taaaacaggc gttgcggatc gaggcagggg ccgatcgact
    16321 gcagcaacgg atttgtcgcg acgacggcga ggatctggtc cgcaatttgg tttgcaagcc
    16381 tcggcgcgtc actgatcttg ctgacgtcct ccggaaggtc cgagagtagt tcgacgaggt
    16441 cgtcgagtga gccgccgccg cggtatgcga aggccctgag cgcgtcagcc agaatacctc
    16501 gtttgagaga ggctttctgg cccgtccctc cgagataagg cgaaagtgtc gcccgcgcca
    16561 tctcgattgc ctcgaaccgc tcttgctcgg tttgctcgtc ccggctgtct ccgattgcag
    16621 caaagtcggg gagcagattg agcgaaatcg gatttccagt acttacgccc ggcgtccaaa
    16681 cgaccacatt ggcgcgctcg ctgtaagctg ctgctttggc gacgtcatcg tcgccgaaac
    16741 cttccggcgg cgttggccag cgatccccga ggcgcaccaa atcgttgttt atgtcgagca
    16801 caatagaggg tatgccgaga agtgcggcct cttcgacgat ccggcgcagc agcacggtct
    16861 taccggagcc cgaaccggcg aatattgcaa tgtgtcgcgg cagcagatcg gcgtcgaggc
    16921 tgacgggatc gccaagtgtg ccacgctcga atcggcggcc gatgaaaatc gcccgtgagt
    16981 tgctctccat tttagggccg ctcgacgcat tcccggctcc cgcggcggtt tcgaagtcat
    17041 gcgggcgcgt tgcaggtcgc gcgccatcgg gcgaggctgt cagaaagggc gggggacata
    17101 ggccagccgt ccggaaaagt gccgtgtcaa acaacggctt gcgagcccgt agccacccgt
    17161 caaatccggc aagatcttgt ttcgccatct tttgaagggc aatgaaggcg cgcaggtcct
    17221 cactcgtcgg
//
LOCUS       DASVDU010000266        95190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_270921, whole genome shotgun sequence.
ACCESSION   DASVDU010000266 DASVDU010000000
VERSION     DASVDU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 95190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95190
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270921"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VF226_17295"
     CDS             <1..850
                     /locus_tag="VF226_17295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7075796.1"
                     /translation="PAELLDRDAAARLLGSDFWYGGMFNPTGGHINPLALARGLAEAC
                     VRQGVVLHEASPVTGYTREGDGWRVRTAQGSVHARAVVLATNAYTDAIENRLEPRISR
                     TVVPVLSWQMATEPIGDNLRATIVPGRQAVSDTRADLRFFRYDARNRLITGGTVLGSH
                     NVEARVVARTTENITQAFPQLAGVRFTHVWSGWIGITEDRFPHFHRLGPDLWSWTGCN
                     GRGVALSVALGRELASAVDGVPAKELAIPLTEPEPIPFHPIARRIAPLILAWYKRRDL
                     AELKLD"
     gene            870..1352
                     /locus_tag="VF226_17300"
     CDS             870..1352
                     /locus_tag="VF226_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX7075797.1"
                     /translation="MQEMPILQRRRIEAMILKHVYEVIRERSGEQEAREVIGKAVSNA
                     AVEHGKGLAESLGRTPDFEDMLAIMPLWTKDDALEIEILEASKDKLDFNVKRCRYSEM
                     YKEMGLGHIGDLLSCNRDGDFCIGYNPNMELTRTQTIMKGASHCDFRYRMKSKTEQGQ
                     "
     gene            1357..2520
                     /locus_tag="VF226_17305"
     CDS             1357..2520
                     /locus_tag="VF226_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX7075798.1"
                     /translation="MAVENWVSRQLDELTEFRRDLHRNPELLYDVERTASKVADALRA
                     AGVDEVVTGMGRTGVVGVIRGNTDTRGRMIGLRADMDALPITEQTGAPWTSTKPGVMH
                     ACGHDGHTTMLLGAARQLAESRAFDGTVIVIFQPAEEGGAGAKAMIDDGLFERFPVQE
                     IYGLHNLPNLPLGQFAITPGATMASVDEIRISVEGIGGHAAFPHQTVDPITVTAALIQ
                     AVQTIVSRNVDPLKSAVVSITTINGGDAFNVIPPVITLTGTVRTLDEGIRDLVEKRLR
                     KAVAGIADAFDAKAELKYVRSYPVTVNNAAKLENAVRAAAEVVGAANVKTDLPPTMGG
                     EDFAFMLEAVPGAFIFAGNGPSANLHHPQYDFNDELIPFGCSYWTTLVRQLLG"
     gene            complement(2749..3963)
                     /locus_tag="VF226_17310"
     CDS             complement(2749..3963)
                     /locus_tag="VF226_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7075799.1"
                     /translation="MQDMTGEKKAAAAKPVFQWDDPLLLEAQLTEEERMVRDTARAYA
                     QEKLLPRVQEAFRHEKTDPSIFREMGELGLLGATIPDTYGGAGLSYVCYGLIAREIER
                     VDSGYRSMMSVQSSLVMLPIYEFGSEEQRRKYLPKLAKGEWIGCFGLTEPNHGSDPGS
                     METRARKVEGGYSLKGAKTWITNAPIADVFVVWAKTEDGVIRGFILEKGWKGLSAPAI
                     HGKVGLRASITGEIVLDDVFVPEENLLPNVSGLKGPFTCLNSARYGIAWGALGAAEAC
                     LETARQYVLDRKQFGRPLAANQLIQKKLADMVTEISLGLQGCLRLGRMKDEGVAPVEV
                     TSILKRNSCGKALDIARMARDMLGGNGISDEFCVARHLVNLEVVNTYEGTHDIHALIL
                     GRAITGIQAFTN"
     gene            complement(4204..5145)
                     /locus_tag="VF226_17315"
     CDS             complement(4204..5145)
                     /locus_tag="VF226_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7075800.1"
                     /translation="MARKRRSALGILIKLLALLLIAGGAALYLLTIPSTLPPSALPQR
                     TADLANGEVMFHAGGCASCHSTPGQEDKTRLGGGLELKTAFGTFHAPNISPDPQHGIG
                     GWSEIAFVNAMLRGVGRDGEHLYPSFPYTSYQRMTLDDVRDLFAFIKTLPAEAKPSIP
                     HELQFPFNVRRAVGLWKLIYLDGRSFEPDPAQDSVVNRGAYLVEGPGHCAECHSQRDA
                     LGGIIPERRFAGGPNPTGKGWVPNITPHEDGLASWSAGDIAYLLESGFTPDYDSVGSS
                     MADVVANTSKLSADDRAAMAAYLKSLPPRPGKRQSSN"
     gene            complement(5269..5709)
                     /locus_tag="VF226_17320"
     CDS             complement(5269..5709)
                     /locus_tag="VF226_17320"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7075801.1"
                     /translation="MMRILSVAVALAVGATIYTAANAETDVIKQRQQAMKAMAGAAKE
                     PGAVIKGEAKFDLDKIRQSLAVYQEKASDLKKLWPKGSNNGETAALPIIWEKQDDFLA
                     RFEKLAADAKAAAASIKDEASFKAEWPKVMSNCGGCHKQYRRPD"
     gene            5918..6832
                     /locus_tag="VF226_17325"
     CDS             5918..6832
                     /locus_tag="VF226_17325"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7075802.1"
                     /translation="MTLTASNTGPDRVFRLRDGRRISYAEFGAPDGRPVLALHGTPAS
                     RLMYAIAASSAARLGLRLIAPDRWGYAGTDPHPEPSLSAWAQDAAELADGLGLDRFSI
                     IGISGGSPYAAGTAAELGSRVIAMALAVPVGPLVSNGSKRRLPLLQRLSFFWVGQRPS
                     LTKSVFKAYGRFLNRWSRRAVSLAMIGQSRFDRELLRKPAINDYFGEISREGLRAGGA
                     GPAIDLQLFSSEWHVPLDRISAKTRIWIGKDDKLVPLAAARELARAIPGAILTELDDR
                     GHFWIAEEYGQVLDWLAAAGRDEGEVRQ"
     gene            complement(6822..7046)
                     /locus_tag="VF226_17330"
     CDS             complement(6822..7046)
                     /locus_tag="VF226_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075803.1"
                     /translation="MGIDKLIRAAEAAGYAMANSEDLLVPAPAPKSDPQPWRPAEPVT
                     QRLEQRDIAAAKPHGGIREWWFALSNRATA"
     gene            7513..9561
                     /locus_tag="VF226_17335"
     CDS             7513..9561
                     /locus_tag="VF226_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7075804.1"
                     /translation="MGQTAGSQGRSARCIALVGPYLSGKTTLLEAILARSGAISRQGR
                     VTEGSTVGDNTPEARAHGMSVELNVAEVEYLGDQFTFIDCPGSIEFQHESSAALTACD
                     AAVVVCEPDPKRVPALQLILKQLEDRAIPHLLFLNKIDTFDTRVRDIIPLLQPASATP
                     LVLRQIPIWENGIAIGFVDLALERAFVYREHAPSEIVEMPQGVQEREQEARFHMLEQL
                     ADYDDELMEQLLSDMQPPRDKVFDDLAKELREGRICPVLLGSAERGNGIFRLLKALRH
                     ESPFVERTAERLGFSGAQSAAYVFKTIYTAHGGKLSFARVLAGEIPDGSTVVGPDGED
                     RIAGLFTVMGQETRKRQNAVAGETVALGRLDHIKTGDTISLDKNGVQQIETPQAPQPV
                     YGIALALKDRKDEVKLSTALGKLIEEDGSLSVEQNPDTHQLVLLGQGEMHLRVALERL
                     NRKYGVAVDSRRRLVPYKETIRKPTTVRGRHKKQSGGHGQFGDVVLDIKPLPRGSGFQ
                     FSETITGGVVPRQFIPSVEIGVQDYLKQGPLGFPVVDIAVTLTDGSYHTVDSSDMAFR
                     QAGRIAMAEGLPQCSPVLLEPIMAVEIAIPSDATAKINALISQRRGQILGFDSREGWP
                     GWDVVQAQIPAAEMDDLIVELRSATAGVGSFTARFDHLAELTGRLAEQVLAQQKAAAE
                     "
     gene            9652..10329
                     /locus_tag="VF226_17340"
     CDS             9652..10329
                     /locus_tag="VF226_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX7075805.1"
                     /translation="MPRPSTDRQLHLSAPEFRRLARERLYPTPSEAIFDPRTGRSWAR
                     SDFDLNPELLADLALMKPPRPAAVLVPIVAREELTVLLTVRTDNLPTHAGQIAFPGGK
                     VEPVDSGPIATAIREAQEEIGLSARYVEPLGYLDSYRSGSGFHIMPVVALVEPSFSLK
                     PDPAEVADTFEVPLSFLMNVANHQQHSREWLGRQRYFYAIPFGDRYIWGATAGMIKNM
                     HERLFGE"
     gene            10326..10598
                     /locus_tag="VF226_17345"
     CDS             10326..10598
                     /locus_tag="VF226_17345"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEX7075806.1"
                     /translation="MIRLIIENTLLFLLPTAIYVAYVLLRRRIEGGNSNVNVLDDAPL
                     IWLVAAGAALVIATLIIFGSVSGGKPGQTYHPPVYRDGRIEPGEIK"
     gene            10606..11865
                     /locus_tag="VF226_17350"
     CDS             10606..11865
                     /locus_tag="VF226_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX7075807.1"
                     /translation="MTAGGIELPRLDQAEWLTNPKTQAVFRAIEAGGYPARAVGGVVR
                     NALLGEPVGDVDIATPAPPEEVMRLADAAGLKAVATGLQHGTVTIVSDHTPYEVTTLR
                     EDVETFGRHARVAFTDDWAADARRRDFTINALYCDARGNIHDPLGGFPDLLARRVRFI
                     GDPAERIREDYLRILRFFRLTAVYAAGPPDEEGLAAAVRERHGLTRLSSERIHQELVR
                     LLAARRASELIGVMRDYGLLTIVLPVAPRPELMRRLIELEQALGRDASAMLRLAALGV
                     EVFEDAVRLRDRLRLSTAEFETLVRAADAPRRGPHPGDDVHQAKVSLYRFGAKAMREQ
                     LLINWARSAAPPDDPQSRSLYGLADAWQPPTFPLSGADVLAAGVPPGPRVGEILGELE
                     NRWLDSDFRLDREQLRAELLRLGSEGD"
     gene            complement(11960..12175)
                     /locus_tag="VF226_17355"
     CDS             complement(11960..12175)
                     /locus_tag="VF226_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075808.1"
                     /translation="MFPLKTLVLAAILAIAFGPSLMACPAHNHMQTATAPAADATVTT
                     ASPASSQSAQAATEVATANPPKAPAAN"
     gene            complement(12409..14046)
                     /gene="groL"
                     /locus_tag="VF226_17360"
     CDS             complement(12409..14046)
                     /gene="groL"
                     /locus_tag="VF226_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602475.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7075809.1"
                     /translation="MPAKEVKFAQEARERLLRGVDILANAVRVTLGPKGRNVVIEKSF
                     GAPRISKDGVTVAKEIELEDKFENMGAQMVREVASKTADQAGDGTTTATVLAHAIVRE
                     GAKSVAAGANPMDLKRGVDLAVNRVVDELRSKSKKITSNEEIAQVGTISANGDAEIGQ
                     IIAEAMRKVGNEGVITVEEAKSLETELEVVEGMQFDRGYISPYFITNPEKMTVELEDP
                     YILIHEKKLSSLQAMLPMLEAVVQSSRPLLIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEDLGIKLENVTLDMLGRAKRVTIDKENTTIVD
                     GAGKKEDIRARTQQIKVQIEETTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKER
                     KDRVDDALNATRAAVEEGIVPGGGVALLRAAKILDELNAENEDQKFGINIVRKALAAP
                     ARQIFENAGEDGAVIVGKILENDKYSYGYNAQTNEYGDLMSQGVIDPTKVVRHALQDA
                     ASVAGLLITTEAMVAERPKKESAPATPAGAGMGGMDF"
     gene            complement(14157..14471)
                     /gene="groES"
                     /locus_tag="VF226_17365"
     CDS             complement(14157..14471)
                     /gene="groES"
                     /locus_tag="VF226_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7075810.1"
                     /translation="MKFRPLHDRVLVRRIEEENKTAGGIIIPDTAKEKPMQGEVLAVG
                     PGKRDESGKIVPLDIRVGDRVLFGKWSGTEVKIDGEDLLIMKESDVLGILEGEGSARA
                     AA"
     gene            complement(14821..15144)
                     /locus_tag="VF226_17370"
     CDS             complement(14821..15144)
                     /locus_tag="VF226_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEX7075811.1"
                     /translation="MASVNLDKMSLKELLDLEAKLEKAISNARERERTEVKQKIEAIV
                     QNAGFSVSELFGGRAPAAKGRQVAAKYVNPDNRSETWTGRGRKPRWLTAKLDKGAKLE
                     DFAVR"
     gene            15699..16988
                     /locus_tag="VF226_17375"
     CDS             15699..16988
                     /locus_tag="VF226_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004577064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX7075812.1"
                     /translation="MLQRYFDLDGHGTNVRTEIVAGVTTFMTMAYIIFVNPQILADAG
                     MDRGAVFVATCLAAALASAFMGLYANYPIALAPGMGLNAYFTYGVVLGLGYSWQTALG
                     AVFLSGILFLVVSLSRIREWVIDSIPLSLKLAIAAGIGMFLAIIGLKSAGIIQASPAT
                     LVTLGELKTLPVALAAAGFLLMVALDARRVPGAIIIGILAVTAAGIALGTNQFGGIIS
                     LPPSIDPVFLKLDIVGALEVGFVAIVFTFFFVDVFDNTGTLVGLAHKAGLVGPDGKLP
                     RVSKALTVDSTAAMAGAVLGTSTTTSYIESAAGVNAGGRTGLVAVTVAVLFLAALFFA
                     PLAGSIPAFATAPALVYVACMMMSAVKEIDFSDSTEYAPAVITIAAMPLTFSIAHGIA
                     FGFIAYAGIKLLAGRLSDVRPAVAILAALFVAKFAFL"
     gene            complement(17244..19364)
                     /locus_tag="VF226_17380"
     CDS             complement(17244..19364)
                     /locus_tag="VF226_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075813.1"
                     /translation="MNWSIDFAPILPWPFIIAGAIVALLLVGLLTFRRSRGTLLRALA
                     LAALVLALMNPTLRQEEREHLGNIALVLMDESTSNTLAGRPEQAKAIRADLEAKLGQI
                     PGLEVRWITAARPDGERTSGTNLFEELNKALANTPPDRLAGVIMVTDGQVHDVPKDAA
                     ALGFDAPVHTLLTGQPDEFDRRIEVLVAPKYAIVGQTRDVELAVRETGKAQSSRGPVT
                     LRVRREGRADEIRRAEINQRVRIPMQFPHAGQNILEVELEAAPGELTPANNRIVISAE
                     GVRENLRVLLVSGEPHAGERTWRNLLKSDAAVDLVHFTILRPPEKQDGTPINQLSLIA
                     FPTRELFSQKIRDFDLIIFDRYQHRGILQLLYYDNIARYVETYGGALLVAAGDDYAGQ
                     MSLVRTPLAPVLPATPTGRVLEQPFQAKLTEDGIKHPVTRGLPGGGDGEHPTWGRWFR
                     QVDVRPERGRIIMKGAEDKPLLVLDRKGKGRVALLTSDHAWLWARGFEGGGPHTDLLR
                     RLSHWLMKEPDLEEERLTASARGLKLTIERRSMEPTVPSVSVLAPSGETSEVTLDEAG
                     PGLWRATIDVREPGLYKMQTQSPTGMLTALAHAGAADPREMSEVTATDQKMRPISEAT
                     GGGVFWTRGGGLFSSRDPGSVDVPRVTMLSSARVLAGSGWMGLKDREAYVTRGVKLTP
                     MFTGFLALAALLVLIAMAWWREGR"
     gene            complement(19583..22468)
                     /locus_tag="VF226_17385"
     CDS             complement(19583..22468)
                     /locus_tag="VF226_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX7075814.1"
                     /translation="MSLGALAFLNPWLLAGLAALPIIYWLLRTVPPRPRQVPFPATRI
                     LVGLENREKTPAQTPWWLMLIRMLAAALVILALAEPVLNPNRQAGLTGSGPVAIVVDN
                     GWASASRWPLRTRMIDQLIDEAESQNRPVVVLPTAFSGRMPTARIEAPNVARTAAAAL
                     KPQPFAPDRQAAVSALEPVLAGQDQVSIVWLTDGIDHGEATSAFLQKLSALGTASLSV
                     IETSVGDEPMGVATNVGRGGRLEATVVRTGGGAREGVLHAFSARGQRLGEAPFRFAPG
                     ETTAKATFELPLELRNQVTRVEIAGEPSAGAVHLLDARSQWNRIGLLSGESSEQAQPL
                     LGPLYYIERALAPFSELVEPGGANLMSGVETVLQRHASILMLADIGTLTGEVAKHLED
                     WVKKGGVLVRFAGPRLEAGGDHLLPVGLRAGGRTLGGALSWSTPQPLAPFSDDSIFAG
                     LDVPKDVMVNRQVLADPTQLSPDVNVWARLADGTPLVTARSLGDGQLVLFHVTANSDW
                     SNLPLSGLFVEMLRRVSTLGRLGGGGEATAADTGAAGTTANDASVLPPVQLLDGWGVL
                     KPPPPMAQAISIAELDSVTPDAEHPPGYYGAQGAPRALNLIHPDTQLKPLPPLPASAQ
                     HRVYEGQVATPMKPWLLALALGLLFADIIAVILLQLGGLALRPVRQAARAAGAIALAI
                     LLIQGATGSAEAQPVSGSAAPSPPTRNNDALALRATSKVTFGYVLTGDPETDEISRAG
                     LSGLGKVLSLRTAVEPGDPIGVNITTDEIAFFPILYWPVPENARSLPEETLAKIDAYM
                     KQGGMIIFDTRDFGQGVPFGFSMRGSDGGTALQRLLGRLDIPRLEPVPEDHVLTKSFY
                     LLRSFPGRWDGGQLWVEAGMNDSLDEGRKARRADGVSSILVTSNDFAAAWAMDDRGRP
                     LYPTVPGGESQREMAYRTGINIVMYALTGNYKADQVHVPALLERLGQ"
     gene            complement(22465..23379)
                     /locus_tag="VF226_17390"
     CDS             complement(22465..23379)
                     /locus_tag="VF226_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7075815.1"
                     /translation="MLALEGEAHALADRLPDLLVEAIRVASTVAHGIHGRRRPGPGET
                     FWQFRQFQSGDAAAQIDWRRSASSDHLYVREREWEAAHTLWLWPDLSPSMYFRSQLAA
                     DAKADRAVVLTLAAAELLVRGGERVALLGLSRPTASRKATSKLAEALAQNIGSEALEA
                     SLPPAERLNRFSGLLLFSDFLEPIETISERLTHIAGDGVTGHMVQVLDPAEETLPYQG
                     RTEFLGMEGSERWLADRVEALRPEYQARLRAHRAALESLAARLGWSFLVHHTDRPAAE
                     PLLTLIMRLQNSGHDYRWAAAAGAGVAG"
     gene            complement(23437..24435)
                     /locus_tag="VF226_17395"
     CDS             complement(23437..24435)
                     /locus_tag="VF226_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7075816.1"
                     /translation="MTTLTHTDDDIVARIEAAGERMSRVHKATASVIFGQDLVIEQTL
                     ITILAGGHGLLIGVPGLAKTLLVETLGKVLGLEAKRIQFTPDLMPSDIIGSEVLEEIP
                     GERRSFRFIAGPIFTQLLMADEINRASPKTQSALLQAMQEHHVTVAGQRYDVPQPFHV
                     LATQNPLEQEGTYPLPEAQLDRFLLQIDVNYPDLVAERRMLFATTGTEERRLEPILTG
                     QELMAMQRLVRQIPVPDKVVEAILRLVRSARPGPDASEDTNRFIAWGPGPRASQALML
                     AVRARALLQGRLAPSVDDVIALAEPVLKHRMALSFVARTEGQEIGGIIARLASVLE"
     gene            24583..25191
                     /locus_tag="VF226_17400"
     CDS             24583..25191
                     /locus_tag="VF226_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX7075817.1"
                     /translation="MTQQASEGANVRIQGLDELIKAAQGDRLPPVQKWNPPYCGDIGM
                     RIRADGTWLYQNSPIGRKPLVKLFSRILRRDADGRTYLVTPVEKVDVTVDDAPFLAVE
                     MQVENRGRDQQLIFRTNVDDIVRCGPEHPLRFACEPGSNGLKPYLLVRGRLEALVTRA
                     LYYDLVELAEPGPGAEGGLGIWSGGAFFALEAEEEKDAGFSP"
     gene            complement(25260..26120)
                     /locus_tag="VF226_17405"
                     /pseudo
     CDS             complement(25260..26120)
                     /locus_tag="VF226_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(26261..26530)
                     /locus_tag="VF226_17410"
     CDS             complement(26261..26530)
                     /locus_tag="VF226_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEX7075818.1"
                     /translation="MVDASFAAQLKGFSLTTAEILYRMPDHPSLLQSFIWQDYDLHPE
                     FPKLAKFLAFWSKNLDGKLYRVRIAHKRLIHPSEVRFVEGEFRLH"
     gene            26832..27425
                     /locus_tag="VF226_17415"
     CDS             26832..27425
                     /locus_tag="VF226_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7075819.1"
                     /translation="MMNPEAVRERILAHAVTAASAHGLKSLSIGEMARDFALPKSRLF
                     TLFPSNEALQIAALEHAAAAFAKDVVEAAPESLPAKERVTTLFERWLDWSRSRRLTRG
                     CPFVHASAEGENLPESVNAKLMEILQHWSGVLRKAIDDAKAQGVVRGDVDTDQFVFEL
                     YGLYLSHHFWHWAMKDPDARARTMRAFDRLLGSALCR"
     regulatory      27491..27603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27697..29574
                     /gene="thiC"
                     /locus_tag="VF226_17420"
     CDS             27697..29574
                     /gene="thiC"
                     /locus_tag="VF226_17420"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7075820.1"
                     /translation="MNKITRQSELMVPKVTTGPINASTKVYDSPEGHPELRVPFREIA
                     LTDPDQPTFRVYDSSGPYTDASATIDVEKGLPRIREAWIRERGNVEQYDGREVRPEDN
                     GNVTGKALARDFPNKARPFRGVEGKPLTQLEYARAGIITKEMIYVAHRENLGRKAVLE
                     RAKSALADGQSFGASIPEHITPEFVRDEIARGRAIIPANINHTELEPMIIGRNFLVKI
                     NANIGNSAVTSSVEEEVEKMVWAIRWGADTVMDLSTGRNIHNTREWILRNSPVPIGTV
                     PIYQALEKCGGDPVKLTWELYRDTLIEQCEQGVDYFTIHAGVRLAYVPLTADRVTGIV
                     SRGGSIMAKWCLAHHKESFLYEHFEDICDIMRKYDVSFSLGDGLRPGSIADANDRAQF
                     AELETLGELTQIAWRKGCQVMIEGPGHVPMHKVKINVDKQLETCGEAPFYTLGPLVTD
                     IAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNRDDVKTGVITYKIAAHAADL
                     AKGHPAAQLRDDALSKARFEFRWQDQFNLSLDPDTATAYHDETLPKEAHKVAHFCSMC
                     GPKFCSMKITQDVRDYAAKQNAPIDVEAGMAEMSAKFRQMGGEVYVDAEKAKEANRAL
                     G"
     gene            complement(29713..30844)
                     /gene="prfB"
                     /locus_tag="VF226_17425"
     CDS             complement(join(29713..30777,30779..30844))
                     /gene="prfB"
                     /locus_tag="VF226_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531367.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX7075821.1"
                     /translation="MRAEPQKHVDQIRQSLALLRRHFDWDTAEARLAELNARAEDPTL
                     WNDARAAQKIMRERQTLERQISSYKQLEQELDDAITLIELGEAEGDEATIAEGEAALK
                     KLSEQAQKREIEALLSGEADSNDAYLEVHAGAGGTESQDWAEMLTRMYIRWAEDHGFK
                     VTMVEASPGEEAGLKSATLEIKGENAYGWLKTESGVHRLVRISPFDSNARRHTSFASV
                     WVYPVIDDNIDIDIKESDCRIDTYRASGAGGQHVNTTDSAVRITHLPTGIVVASQQER
                     SQIKNRAIAWAMLRARLYDLELKKREEKANAEAAAKTDIGWGHQIRSYVLQPYQLVKD
                     VRTGVESGNPQAVLDGDLDPFIEAALAQRLKDDRPAETTDAG"
     gene            complement(31030..33558)
                     /locus_tag="VF226_17430"
     CDS             complement(31030..33558)
                     /locus_tag="VF226_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX7075822.1"
                     /translation="MRAPILPPPATPKRKRKRRKSVLLSLLGWVFAAGVVAFVAGSAA
                     AGYLLWSVSRDLPSYESLAQYEPPVMTRIHAHDGQLIAEYARQRRIFVPINTIPKLVI
                     HAFLSAEDKRFYEHGGIDFFGIVRAFIRNLQNPGRRPEGASTITQQVAKNFLLSSEQK
                     YERKLKEAILAIRIERAFSKDKILELYLNEIYLGIGSYGVAAAALNYFNKELDELTPE
                     EAAYLAALPKAPNNYHPFRNTREATARRNWVLEQMAENGYITHEQMEAAKAKPLQVNL
                     RPFGAHIFAAEFFAEEVRRTLLAKYGEDKLYGGGLSVRTTLDPDLQGYARQALMDGLV
                     AFDRQRGWRGPVDKIDVTADWGATLAKMDVPSDIQPWRLGVVLQVERDKIIVGLRPDR
                     QPNGKIPEQREAVEIAFEEMKWAGPFRATDKPKGPAPKKPADVVAVGDVIYVAPKDPN
                     EPTGAWSLMQIPEVGGGIVAMDPHTGRVLAVVGGFSFSISQFDRAMQAKRQPGSAFKP
                     LVYAAALDNGYTPTSIVLDAPIEIEQGPGQEVWRPQNYNKEMSYGPSTLRIGIEKSRN
                     QMTARLAQELGMPLIIEYAKRFGAYDDLLPVLAMSLGAGETTLLRMAAAYSVFANGGK
                     KVRPTLIDRIQDRWGRTIWRHDQRECPDCDADAWRGQEEPEIPDDRKQVLDPQTAYQM
                     TSILEGVIQRGTATNVKRVLPGTPLAGKTGTTNESKDAWFVGYSPDLVVGVYVGYDTP
                     KPMGKSATGGQVAAPIFANFMKMALDDKKVPFRAPPGIRTVPVNRYTGLRASPGEKDV
                     IWEVFKPGEEPNDPASFLGYETSSYGDGVSPPPRNVSSGFGNVY"
     gene            complement(33869..35134)
                     /locus_tag="VF226_17435"
     CDS             complement(33869..35134)
                     /locus_tag="VF226_17435"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7075823.1"
                     /translation="MLAATMAALIGLNLVGAACVLAQAGRATGWTIEVNSEGPAGRPA
                     VTRAHLTANDKLTRLELELTREVAISVSTMTEPDRVIVDLPEVDFRIASTVGQNGKGL
                     IKAFRYGLFGPRQSRVVIDTDGPVHVRKARVETSGQTARLVIELAREAIRPAPDTPAV
                     DAAPVLRPSKFEEPPPAVTLRKGKPVVVVDAGHGGVDPGAIGPDGLPEKDIVLSVARR
                     LRNLLVRSGRYTVVMTRNRDMYISLEQRVRTSRRHGADLFISIHADSVAEESLAPHVR
                     GGSIYTLSDRASNEEARRLADKENAADLLAGLESTADQEEDEVRDILIDLLRRETENF
                     SADFSNLLTGELRKHIVLARDPQRSAAFQVLKQAETPSVLVELGYVSNPEDRKLLTSP
                     AWQDKVAGAMLAAIDSFFARRAMGMVHAP"
     gene            35963..38947
                     /locus_tag="VF226_17440"
     CDS             35963..38947
                     /locus_tag="VF226_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7075824.1"
                     /translation="MSSNKMLIDATHPEETRVVVLRGGRVEEFDFESAARKLLRGNIF
                     LAKITRVEPSLQAAFVDYGGNRHGFLAFNEIHPDYYQIPVADRQALIDEEAAAEAEDE
                     AAADRQAEERSQTHGDREEDHPDGEGPEEVGVDVTAFLPPEPEAPSEPQEAAPSAETA
                     GPPEEEAGSRTITEAVQPEERLTEAVPSGTAENGHVAEHSESREDDSGARADASGAHE
                     PRSDENGSDASEEALSDAPKPIGLVEEVGVEEVEENGNGNGGVEQVDNVVAEDALEEL
                     PSRPRRRQRSYKIQEVIKRRQIVLVQVVKEERGSKGAALTTYLSLAGRYTVLMPNTAR
                     GGGISRKITNVQDRKRLKAIAAELDVPEGMGLIIRTAGASRTKQEIKRDFEYLLRLWE
                     SVRELTLRSTAPALVYEEGDLIKRSIRDLYTKDIDEIIVAGEAAYKEARDFMRMLMPS
                     HVKNVVLYQDPEPLFSRYQIERQLNAMFSPYVHLKSGGYLVINQTEALVAIDVNSGKA
                     TREFSIEETALNTNLEAADEIARQLKLRDLAGLIVIDFIDMEEKRNNRAVERRLKEAL
                     RFDRARIQVGRISHFGLLEMSRQRLRTGVLEGSTSQCPHCQGTGIIRSTESVALAVLR
                     AMEDALMSGPPAPIVAKTTASVALYILNNKRSFINEMESRYKVPIYVQASDKAQGANF
                     FIEKAARSADAQRQPERSAVSMDWGFEGEEEPEPAAREEEVSHHEAEDGEGARRSRRR
                     RRRRGRRSERGESLGVTAVPGNGADEEIAYSSEGADTEDDVGAAEAAEDEAGAEVGAA
                     AVEEEGEAHEDRRNRRGRRRGRRGGRRDRGEGSRPGAEAAEAAGEVEPDEAEREAAEP
                     ELGLPVAEPVAERAPAAAVAAQPLATESAETVAEAAPQPPAAAAEAASEEPAPTSPVE
                     EPEAQPLAAAAEPAPAEDQIPELQAGEARPGRNARTEPVASEPVIERIVVGPEIGTAE
                     QPAPAEPVRRGWWQRRFGATTE"
     gene            complement(39053..40144)
                     /gene="dctP"
                     /locus_tag="VF226_17445"
     CDS             complement(39053..40144)
                     /gene="dctP"
                     /locus_tag="VF226_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7075825.1"
                     /translation="MKRRTFLKAGAVSAAASALASPAIAQSAPEIRWRLTSSFPRSLD
                     TIYGTAQTFAKYVAEATDNKFQIQTFPAGEIIPGLQALDAVSVGTVECAQTPVYFYIG
                     KEPTLAFGTGLPFGLNARHQHSWWHFGGGREIINEALGKFNVYGIVMGNSGTQMGGWF
                     RKEINTVEDLKGLKFRIGGMGGHVLARLGVVPQQIAPGDVYPALERGTIDATEFVGPY
                     DDEKLAFHRVAKYYYYPGWWEGGAMMHLIVNMEKWQSLPKTYQAIVNNAAEAANNWMI
                     AKYDLVNAPALKRLIAAGAELRAFPQPVMEACWKAANELYAEIAAENELFKRALESHH
                     AFRNEVLYFWQIAEHYYDSSLMAAMRSKG"
     gene            complement(40355..41029)
                     /gene="aat"
                     /locus_tag="VF226_17450"
     CDS             complement(40355..41029)
                     /gene="aat"
                     /locus_tag="VF226_17450"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX7075826.1"
                     /translation="MTARESPLLEITPQVLLKAYTCGIFPMAESADDPQLYWIEPQAR
                     GVLPLDRVLVPKRLQRTIRQAPYEIRIDTDFDGVIAGCAASRPGRRTTWINDRIRKLY
                     RELHRLGHCHTVEAWHQGQLVGGLYGVALGGAFFGESMFSEMRDASKIALVYLAARLI
                     FGGFVMLDTQFVTDHLRQFGTIEVDRSEFHRQLERALQTDGDFFRLEREPTPERVLDI
                     VLRRKG"
     gene            complement(41239..42582)
                     /gene="accC"
                     /locus_tag="VF226_17455"
     CDS             complement(41239..42582)
                     /gene="accC"
                     /locus_tag="VF226_17455"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7075827.1"
                     /translation="MFDKVLIANRGEIALRIQRACKELGIATVAVHSTADADAMHVRL
                     ADESVCIGPPPAAESYLNIPRLLAACEITGADAVHPGYGFLAENARFAEILEEHGITF
                     IGPTSEHIRIMGDKIEAKETAKRLGIPVVPGSDGAVTSEADAQRIARDMGFPVLIKAA
                     AGGGGRGMKVARSPDDLEVALATARSEAKAAFGDDSVYLEKYLSKPRHIEIQVFGDGK
                     GNAIHLGERDCSLQRRHQKIWEEAPSPALNAEARQKIGETVAAAMRKLKYRGAGTVEF
                     LYQDGEFYFIEMNTRLQVEHPVTEMITGVDLVIEQIRIAAGLPLSLRQEEVSFSGHAI
                     ECRINAENPRTFRPSPGKITYWHPPGGLGVRVDSGVYQGYRIPPYYDSLIGKLIVHGK
                     NRNECLMRLKRCLSEFVIDGIETTIPLFVDLVKQADIANGLYDIHWLEKYLGNAE"
     gene            complement(42632..43108)
                     /gene="accB"
                     /locus_tag="VF226_17460"
     CDS             complement(42632..43108)
                     /gene="accB"
                     /locus_tag="VF226_17460"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX7075828.1"
                     /translation="MAKDQRDRGGNSEQQMIRDLAALLVETGLSEIEIEKSGLKIRVA
                     RNLSVSAVAPQQAAPASPVSPAGQAGPAEPASDIANHPGCVKSPMVGTAYRAPEPGAA
                     PFVEIGSRVVQGQTLMIIEAMKTMNHIPAPRSGTVTHILVENGQPVEFGEPLVVIE"
     gene            complement(43158..43616)
                     /gene="aroQ"
                     /locus_tag="VF226_17465"
     CDS             complement(43158..43616)
                     /gene="aroQ"
                     /locus_tag="VF226_17465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX7075829.1"
                     /translation="MAKPVYVLNGPNLNMLGLREPSIYGAETLGDVQRRVEARAAQLG
                     LQIDFRQSNIEGELVTWIQQARNAASGIILNAGAYTHTSVALLDALSAAELPAIEVHL
                     TNVFRRESFRHHSYISPVARGVICGFGPKTYELALEAMADILEQQGQARP"
     gene            complement(43754..44599)
                     /locus_tag="VF226_17470"
     CDS             complement(43754..44599)
                     /locus_tag="VF226_17470"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX7075830.1"
                     /translation="MPAKSLIKIIDALTTRRGAVAGAIALLALGSALALGSAPAVSTG
                     ANIKVAQADTSGPFSAEQKQAIERIVRDYLVKNPEVLLEVGRELEKRQAEMQVAEHRR
                     IILENKDKIFTSPADFVLGNPNGDITIVEFFDYNCPWCKRAVGELMKLIEADSNVKVV
                     LKEFPIFGENSIFAAKAAMASRAQGKYLDFHIALMKERQVTKDNVFKIAQKVGLDVEK
                     LKADMDNPEFEAAIKQTSAIAQALAIEGTPGYVVDAKVNVGFLALDGLRQMIGEARKE
                     GCQVC"
     gene            complement(44739..46115)
                     /locus_tag="VF226_17475"
     CDS             complement(44739..46115)
                     /locus_tag="VF226_17475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7075831.1"
                     /translation="MLQNRKTGRQTPLSACLVATALVAVLAFTSVTSKPALAQGLPLI
                     RDTEIENLLNDYARPIFKAAGLGENRVAVRIVRSGMFNAFVLDGRNVFVHTGALMQSE
                     TPNEIIGVIAHEAGHIAGGHMAALRARIARDQTRALLMRMLGLGVAIATGAGEAVFAG
                     DELIMRSLLAERRSQEAAADQAGLRYLNATRQSGRGMLETFERFAQQEYISDAQKDPF
                     VRSHPVAADRLARLRELVQRSPYYDNRDSSELQLRHDMMRAKLAGYLERPQVVFNRYP
                     RSDTSLPARYARAIASFMQGGQSALPAALAQVDALIREVPGNPYFYELKGDFLTRSGK
                     NREAIAPLRQALRLSKGAPLIRVQLASALLASNDPKVVGEAADLLRKAVVEDDNPRAY
                     RSLADAYYRLGRQADADASIAQAYFLEGNIKQAQTFAQRAQRALKKGSPAWLRMDDII
                     RYKDASNL"
     gene            46469..47581
                     /locus_tag="VF226_17480"
     CDS             46469..47581
                     /locus_tag="VF226_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7075832.1"
                     /translation="MDVMRAAAAREAAGHRVIHMEVGQPGTPAPATARAAVERAMATD
                     NLGYTLALGMPSLRERIAKHYRDWYGVELSPERVVVTNGSSAAFVLTFLALFDAGERV
                     ALPSPGYPCYRHILTALGQQPVIIEADESSRWMPTPDQIEAASARDHIAGVLIASPAN
                     PTGTMLEPQRLKDILKVCRRRGLWFISDEIYHGLTYGMPAETALKHSDGVVVINSFSK
                     YFSMTGWRVGWMVVPEELVRPVERLAQNLYIAPPTVSQVAALAAFDGIEELEANRRVY
                     AANRDLLLRELARAGFEEIVPADGAFYLYADVSRFTDDSLAFAKLMLEETGVATTPGV
                     DFDEARGRHFIRFSYAGTTADMAEAAERLKGWTRLK"
     gene            complement(47591..48589)
                     /locus_tag="VF226_17485"
     CDS             complement(47591..48589)
                     /locus_tag="VF226_17485"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7075833.1"
                     /translation="MDIQDLRIFARVAAVQNLSSVGTELGLTPGTISKRIQALESELR
                     VRLFDRTTRSIRITEEGATFLTHVERVLAELESARAAVGDHATKPRGKLKISAPATLG
                     RLFVAPAISSFMRAYPDIEIQMNLTDRNVNLQEEGYDVAIRTGALTDSVLIAKRLCPD
                     RQVVVAAPSYLAANGTPQTPADLSAHNCFVLGDNWQWSFCRGKEDFTIRVNGRLRSNN
                     GEILHHAALDGHGLFQTSELRVREDLAAGRLVRVLKEFELASNSAVWAVYPSSKHVLP
                     KLRVLLDFLAVWFRDVSAERPRTPKRSHNKRPEAANNLQNAPSHLPPDRQPGTVLP"
     gene            complement(48810..51398)
                     /locus_tag="VF226_17490"
     CDS             complement(48810..51398)
                     /locus_tag="VF226_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEX7075834.1"
                     /translation="MSKDQQTPSAEGRAFNRKVRLSRLALLFEQLWPRLWLVIGVLGL
                     FVLVSFAGLWLWLPPVAHEIVLSAFGLALLAALIFAARVRWPTREEGIRRIERRSGIP
                     HRPASSYEDTLSASASGSATERIWQAHKARLAALLERLRVGPPQPRTDRKDPFALRAL
                     LVLGVVLLLGLVGDSAKDRLFAAFRFGPDLQLANARLDAWVTPPAYTGKPPVLLADGG
                     SEPSRPSHAEAGPIEVPDKSLLIIRASGVGQLSLELGEQNSEKPQIIEGRQPKQAGDI
                     SEVRAELRKSGTVRVLGGGSEVVVWRFQVTPDQAPRIVLTKEPERTIRGGMKLFYKVE
                     DDYGVASAEAHFARPRTVAGDPRTSWAREDILQGPRPPLERPPVLNLRLPRQNAKDSE
                     TSSFHQLGAHPYAGMKVIMTLVAKDHAGNVGKSQPIEIVLPQRRFTKPLARAVIEQRL
                     KLVEDPRYRELVSKALDALTFEPEGFIEDHNVYLGLRSVYHRLLQDSSREGLRSSVDQ
                     LWHVALRIEDGGELTDAERRLRDIQDQLSKALEEGASDEEIARLMQELRQAMAEFLQQ
                     LARQAQGQQMQLPEGFNPNQIMRPQDLQRMLDQLENMARQGSREMAQQMLDQLRELME
                     SLQAGRMMGQQGQQGQQMMQMMDQMGDIIGKQQQLYDDTFGEQQRQGQEGQRGQRGQR
                     GQQFGQRGQGQRGQQGRGQQGQRFGQGELTERQGQLRDRLGQLREGLQQFGMRPPSQL
                     DGAEQSMENARRALEQGDLDTATREQSQALEQLRQGAQSMAEQMLRQMPSRFGQGPAG
                     DAPRDPLGRPQRTEGPDLGTSVKVPDEIDVQRAREILEELRRRLGETTRPALELDYIE
                     RLLRRF"
     gene            complement(51527..52801)
                     /gene="lysA"
                     /locus_tag="VF226_17495"
     CDS             complement(51527..52801)
                     /gene="lysA"
                     /locus_tag="VF226_17495"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7075835.1"
                     /translation="MHHFSYRAGVLHAEGVSLARIADEVGTPFYCYSTATLERHYRVL
                     SEAFRGTDHLICFAVKANSNQAVLRTMARLGAGMDVVSEGELRRARAAGVPADKIIFA
                     GVGKTRDELTYAVSEGVLGFNVESEPELELLSEVASQQRKTARVAIRVNPDVDARTHA
                     KISTGKSENKFGIPFLEAPKLYAQAARLPGIRIAGIHMHIGSQITDLKPFRDAFRLMR
                     ELVLALRREGHELEHLDLGGGLGVPYRGSDDVPPHPDEYAATVRAALGDLGLKFILEP
                     GRMIAGNAGVLVSRVIYSKRGAAKTFTIVDAAMNDLIRPTLYEAYHDIWPVREAKLQL
                     ERQRQDVVGPVCETGDYLALGRELPPLETGDLIAVMTAGAYGAVMSSTYNSRLLVPEV
                     LVNGDDYAVVRPRPTYDDLIDLDRLPDWLTDA"
     gene            complement(52961..53446)
                     /locus_tag="VF226_17500"
     CDS             complement(52961..53446)
                     /locus_tag="VF226_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX7075836.1"
                     /translation="MDNAEKPFWKQKTLEEMSEAEWESLCDGCGRCCLLKLEDEDTGE
                     IYRTRLACRLLDLGTCRCSSYADRQSHVPDCVSLTPEKARTLSWLPETCAYRVLANGG
                     DLAWWHPLVSGDPETVHQAGISVRGWVMSEERATLETMHRYIIEDYDRPRRRARARQG
                     S"
     gene            53540..55762
                     /locus_tag="VF226_17505"
     CDS             53540..55762
                     /locus_tag="VF226_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7075837.1"
                     /translation="MYDWFFKQRGRDRLINWLGLDAWIDSTLAETWERIKDGYDAASS
                     FFARFRLTGWRRLLNEAACEALTLATGGFFIMYGLALPAFMEVEDGKWLTTGQYSVKF
                     LDVNGNEIGKRGINLDDAVPLEEIPDYMIKATLATEDRRFYEHFGVDVFGTFRAFIEN
                     LRANDVVQGGSSLTQQLAKNLFLSSERSLQRKIKEAFLALWLESRLTKDEILKLYLDR
                     SYMGGGAFGVEAAAQFYFGKSIRDVNLAEAAMLAGLFKAPTKYAPHVDLAAARARANV
                     VLNNLVEAGYYTAGQVQHARLNPARVVETRHADSPDWFLDYAFEEVQRLMAGKGHFNL
                     IARTTVDLSLQEAAEKALVSALRQRGRAVRADAGAFVVTETDGAVRALVGGPDYGESQ
                     FNRATKARRQPGSSFKIYVYATALENGYKPTTIVRDASRSCGHWHPQNYNGSHGSGAR
                     MPLWLALAKSLNTTAAELSFAVGREKVIEMTQRLGIQGVQKSCSMALGDTGITPLQHV
                     GGVATFANGGRLAKPYAITEIYTASGELVYSRERDEPPAPQVISREVVEQMNQMLFKV
                     VEEGTGQRAKLDFTHVAGKTGTSTGPKDVWFVGFTGKYVGGVWIGRDDNRPMSGGTTG
                     GQLAAPIWQQFMSVAHTDMNIPTIPGLSPHPIQVAEQQRVAAMQRNKVAASEPAAGPR
                     SQSLMSERTRKVLERIVKSLRTASGDQGTEEPAPGAPAADRRADAARWFEDLPIRGRP
                     "
     gene            55759..56391
                     /locus_tag="VF226_17510"
     CDS             55759..56391
                     /locus_tag="VF226_17510"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX7075838.1"
                     /translation="MTVAVASEAPARFARLRATLGAILGFIGNIGLFAGIALIGGIGS
                     SWYMIEVGSPLTTVKSGPWVSWPAAGTPTADPYTRARVVRHGSLPISGTVARIWEART
                     DNTGQRLHSSCDYLVTGRGLDDGWWTIAVYDDNGHVIPNPADRHAFNSSTIMRSADGS
                     FVVSLARDARPGNWLPTGNAGRLTLVLTLLEAGDGLGGAGLPEIQKVACR"
     gene            56388..56984
                     /locus_tag="VF226_17515"
     CDS             56388..56984
                     /locus_tag="VF226_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX7075839.1"
                     /translation="MSYGWRTGGLGTALLCGVVGAGIVHIVATFATPGLLRPNTYDRI
                     TEHLPANTLVVLGQPEADSQLIPYQEPDMQIAVCHYDVSGGPVSARVVLPSSGWTLAL
                     YSPAGDNFYVMPGRDQRVTMVNALLMLHQNEDAALPLSAGQTARNAPTYIDVPEPTGL
                     MVIRGPVKGESYKDEVANILARASCGPAPNRLQSSSAG"
     gene            complement(56997..57188)
                     /locus_tag="VF226_17520"
     CDS             complement(56997..57188)
                     /locus_tag="VF226_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075840.1"
                     /translation="MGTILSFSPRHDRSDRREEAESQPCRCEIVIFPGVRYERTVTCP
                     DQERATGKAKRRDFLLIPD"
     gene            complement(57301..57663)
                     /locus_tag="VF226_17525"
     CDS             complement(57301..57663)
                     /locus_tag="VF226_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEX7075841.1"
                     /translation="MRLKAEIWAKAYLRSCAAAGVAAAVVRHGDDDAGAIFIKVNRLD
                     GTARLFGPAPAGFDTGQFERQWIAHLDGPEATEAEVDAYLARQVAFDGDLWILELEDR
                     QGRHFLDPWLASSGADDN"
     gene            complement(57718..58395)
                     /locus_tag="VF226_17530"
     CDS             complement(57718..58395)
                     /locus_tag="VF226_17530"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX7075842.1"
                     /translation="MLARRAKIVLCACVAACAGVWWNVMYLQQGTRADILARTQRLGE
                     TARRPPPPALIEPGKAHPTVEIPSVASVIPEPEIDADVVRAVQRELALRGYDPGPADG
                     LIHPVTRAAVMAYEHDHGLPLTARPSEEILKSILFGVPADASGKVVGEPQPQAQEIIR
                     SVQESLAGLGYPISKVDGRMGEETVRAIRAFEAKNDLTPSGRVSGALLLKLRDVAVAR
                     QRRASAR"
     gene            58595..59047
                     /locus_tag="VF226_17535"
     CDS             58595..59047
                     /locus_tag="VF226_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEX7075843.1"
                     /translation="MSITDIADIRSDFALLDEWEDRYRYVIELGRSLPPLDESARTDA
                     NKVRGCTSQVWLITEVVPPADGTGQPRLRFQGDSDAHIVRGLVAILFSIYQNKTPEEI
                     LAIDARGILAELGLQEHLTPQRSNGFASMVERIRADARKAAEGALRTN"
     gene            complement(59462..60664)
                     /locus_tag="VF226_17540"
     CDS             complement(59462..60664)
                     /locus_tag="VF226_17540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7075844.1"
                     /translation="MGFFADSLNRIQPSATIAVSTKARQLKAQGRDIISLSAGEPDFD
                     TPVNIKEAAIRAMNEGKTKYTDVDGIPELKAAIVAKFKRENGLEYKTSQVSVGTGGKQ
                     VLYNALMATLNPGDEVVIPAPCWVSYADIVLLGGGKPVFAPCRIEDGYKLQPETLEKA
                     ITPQTKWFIFNAPSNPTGAAYSKEELKALTDVLMRHRHVWVLSDDMYEHLLYDGHTFW
                     TPAQVEPGLYERSLTLNGLSKAYCMTGWRLGYGAGPEPLINAMRKLQSQSTSNPNSIT
                     QWAAVEALNGPQDFIERNNKVFVQRRDLVVSMLNQARGIRCPKPEGAFYVYPSCEELI
                     GKTTPKGVKINNDEDFVTALLDEEGVACVHGAAFAMSPFFRISYATSTDALEEACRRI
                     QRFCASLT"
     gene            complement(60811..61785)
                     /locus_tag="VF226_17545"
     CDS             complement(60811..61785)
                     /locus_tag="VF226_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7075845.1"
                     /translation="MARPALLMTGHMMALIEEQLDQTFDVHRLKEPADLEAVVARVGE
                     SIEAICTGGHTGVKVDDALMGRLPKLRIIGNFGVGYDSVDAPAAGKRGIIVTNTPDVL
                     TEEVADTTIGLLLMTVRELAQAERWLREGKWAKHGDYRLTPASLRDRSVGILGMGRIG
                     MAIARRLEAFGLPISYCTRRRRTDLPYTYYPNAVELAKAVDTLIVITPGGPETKCIIN
                     AAVLEALGPRGVLINMARGSCVDEAALMKALRDGTILAAGLDVFVNEPNIDPELLTLP
                     NTVLLPHVGSASVHTRNKMGQLVVDNLKAYAERKPPLTPVAETPFKGW"
     gene            61899..63518
                     /locus_tag="VF226_17550"
     CDS             61899..63518
                     /locus_tag="VF226_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7075846.1"
                     /translation="MSEPQYDVAIIGGGHNGLTCAAYLAGAGLRVIVLEKNDVVGGAA
                     VTEEFHPGFRNSVASYTVSLLNPKVIADLELEKHGLKILERTAANFWPIDAERGLLMP
                     YGLANRQRAVAEFSARDAERLPAYDAALDRAANVLRELVLRTPPNAGGGIVELVKAAG
                     LGRRLLGLSLKDRRLLVDLFTKSAADYLASWFESEIIQGAFAFDGIVGAYASPFTPGT
                     AYVLLHHCFGEVNGKPGVWGHAVGGMGAITQAMCASAQARGAVIRTGATVAKINCEHD
                     RATGVVLQSGEEIRARVVAANVPPKLLFRDLVPETAVAPELRARFTHMKTGSGTFRMN
                     VALSELPDFTCRPGKDPQPHHGSGIIIGPTVSYLERAYLDARLYGWSREPVVEMLIPT
                     TLDPALAPPGKHVASLFVQHVAPRLPDGRSWDDAKDEFADLVIETVTRHAPNFKASII
                     ARQVLSPLDLERRFGMVDGDIFHGQLSLDQLFSTRPVLGHADYRMPVAGLYLCGSGAH
                     PGGGVTGAPGHNAAREILRDLKGWFGRAGFG"
     gene            63665..63892
                     /locus_tag="VF226_17555"
     CDS             63665..63892
                     /locus_tag="VF226_17555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075847.1"
                     /translation="MYGQITKFRPEIGTGVIRAEDGRKYRFVGSELMNRGRELVGQGV
                     DFVLVERRPREIIVLSGSPWTAFGANPRRPN"
     gene            63919..64344
                     /locus_tag="VF226_17560"
     CDS             63919..64344
                     /locus_tag="VF226_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075848.1"
                     /translation="MAITWDPTDVPTVAAEDLAAPMRRLIAEGRGLVLLRGLTAEDAK
                     IVEECLRQRLGKEPSVELAALMRFRALVDVFACRRLSELLLERGHEVIAPSLQCAAQM
                     RLSLKWGFNPRKFHRVVTELLDGGDAGIYFVSRELLDAA"
     gene            complement(64354..64662)
                     /locus_tag="VF226_17565"
     CDS             complement(64354..64662)
                     /locus_tag="VF226_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075849.1"
                     /translation="MSKRKSTSAQLRGDIDRGLTGDKVAAADPAAAPLGTDEEAAGTP
                     LSPEVIAEAHERELQIGRLARALPPARVVSVQTRGVAPWLAIAAFLLAILAVAWFASQ
                     "
     gene            complement(64798..65175)
                     /locus_tag="VF226_17570"
     CDS             complement(64798..65175)
                     /locus_tag="VF226_17570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075850.1"
                     /translation="MRSLVLLGGLVAALACASMAAQPSYAEGYRAWDEGRDYRPAYYP
                     APRPRTVSGYFVADPYAYRYEPRGYYPYYDAGYWRPICRGKTVCVPRPYQPPYWKAWG
                     YPKPWLNREFHWIYHGRIRPWHW"
     gene            65364..65439
                     /locus_tag="VF226_17575"
     tRNA            65364..65439
                     /locus_tag="VF226_17575"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:65397..65399,aa:Thr,seq:tgt)
     gene            65847..66215
                     /locus_tag="VF226_17580"
     CDS             65847..66215
                     /locus_tag="VF226_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7075851.1"
                     /translation="MIDILIAEDEPAILESLNFILGRCGWIVDSVRDGEEALDAVRRH
                     KPRLLLLDIMLPKRSGLDVLKSMRADPRLNHIPVLILTARGQQHDRQLAQELKADSFV
                     TKPYSNEEVIREVRRLLDRG"
     gene            66228..66521
                     /locus_tag="VF226_17585"
     CDS             66228..66521
                     /locus_tag="VF226_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075852.1"
                     /translation="MAKRPFVKEEDARKAAEAVFFAQICGALLLLPPIINLFNKRLSL
                     FGVPLEVLYLFLVWIVLIASAVVISASVPRASADEQLGEEPAQPTNESGKEGG"
     gene            66521..69271
                     /locus_tag="VF226_17590"
     CDS             66521..69271
                     /locus_tag="VF226_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7075853.1"
                     /translation="MLSPDFVILTAIAYVGFLFILAYVSDARAKRGKSGIFRSPFVYT
                     LSISVYCTSWTFYGAVGTAARDGLEFLAIYLGPTLVFVGWWLILRKLVRISHSQRITS
                     VADLLSSRFGKSSRLAVLVTLIAVVSVTPYIALQLKAITSSIQAVSSATGSSGGTLVG
                     LDDVRLAFGIATVMAIFTILFGTRNVDAREQHHGVVAAIAFEAAVKLVAFIAVGIFVV
                     YGLGGGFEKVYARALEAGIDIHEKGDFGTRWVAVLVLSSAAVICLPRQFQITIVENSD
                     ENHLRTAGWAFPTYLFLMSVFTLPIALYGLATRPADSNPDMFILALPMSAGNDALALF
                     AFIGGFSSATSMIIVASIALSIMVSNHVVMPIALRRSNNLEATGDRDIAQLLLRSRRI
                     SIAGMLFLGFVYFWLTGDSGALAPIGLISFAGVAQFLPSMLAALYWRHATVHGAISGT
                     LVGFITWAWTTFLPSFQSTSPAIATLMAEGPWGISILRPQALFGMEGFDPLVHSVFWT
                     LLFNAGTLVCVSLATSQSALERIQATLFVDIFRRHPGSQPQILRGSATTDELFFVAER
                     VLGWQRAKELFETSDVPHRGASLPFGVSSAFITKLEREMAGSIGAASAHVLLSKVVAG
                     DSVSLEEMMRIADETQQVIEYSHELEQKSIELRSTAQQLREANSLLQELHRQKDDFLS
                     QVSHEVRTPMTSIRSFSEILLMDEDLNADQRQRFVSIIHQESVRLTKLLDEILDLSAL
                     EHGESDWENTSVDAEQVLDRAIAVCEALARQKSVTVESRSRARAGRVYANADRLSQVF
                     INIIANAISHNNAEDPKVQITSWTTAETYVVEICDNGPGIPKEYRRKIFDKFVRASPA
                     ADVSTPTSGLGLGLNISYTIVKKMNGALELVDGHLPGACFRIVLPLEGVGVKAERARV
                     SG"
     gene            complement(69365..70909)
                     /locus_tag="VF226_17595"
     CDS             complement(69365..70909)
                     /locus_tag="VF226_17595"
                     /inference="COORDINATES: protein motif:HMM:NF021382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX7075854.1"
                     /translation="MRAPVGIQIRRRRNALNLSQAALARAVGISPSYLNLIENNKREV
                     GGRLLLRIAERLNLDVDRLSGDAEQRTIHALEELLSEPLLNGLEIDAASIRDLVARHP
                     EAAHAMARLHRAYIDSVAGMEAYANRLKSDPLLSEMLHQVLNRIAGIRSSAEILSGVP
                     DLTEADRDRFLRNINGEAEDLTGILKNLVSYFDQTASRQRIVSPLREVEDAIIAANNH
                     FPELEINADFLRREVERNGIISEPDLGDALRRRFGIECRSGQAFTGQHSFDPQSRTLW
                     FRVSTTPATRRFQMCRIYAEHAAPELLEDQVKRLSLTTKEASQLARRALSSYVAGAMV
                     MPYDAFLNDAESRRYDIDLLSHIYDASFEQVAHRFVTLRRKGAEGVPFGFLRADPAGR
                     LTKRFPIPGLILPILGHGCPLWPIYSAPSTGKVVRQISEFTNGARYLLIAKAVTKQIA
                     AYQEQPITFSIMLACDILHADRIVYGQGLDLADSRNRVLVGPSCLLCSRQDCEHRQEP
                     APATII"
     gene            71093..72433
                     /locus_tag="VF226_17600"
     CDS             71093..72433
                     /locus_tag="VF226_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HEX7075855.1"
                     /translation="MSKSKVSRRSVLKGAAGLAAITGSPYIFSKTAWAENYCNAPGKT
                     AIFGFNVPQTGSYTEEGLDQLKGYQLAVKHINGEGDGGLLNTLKPRALKGNGILGKKV
                     EYVTGDTQTNTDVARDNARRMIERDKAIMVTGCVTSAEAIAQQALCQEKGVIYMSGLT
                     HSNDTTGKDKRRYGFRHFFNAYQSGVAIAPILAKEYGKDRRAYHLTADYTWGWTQEES
                     IKNATEALGWQTVATVRTPLGLGDYSQYLTPVLNSGADVLILNHYGHDMVNSLPQAVQ
                     FGLRDRQANGKNFEIIVPMFSELMAIGAGDAVKGIIGTSNWHWALDDEGTKAFTKSYG
                     EEYGAPPSQSSHTGYVQAILYADAVERAGTFYPPEVIKALEGFEFDGMGNGPTLYRDS
                     DHQCFKEVLVVRGNENPSSKFDVLQVIGKTSREEVTYDPAIFGGELGPYEPNQC"
     gene            72523..73551
                     /locus_tag="VF226_17605"
     CDS             72523..73551
                     /locus_tag="VF226_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075856.1"
                     /translation="MDAFLAQLLNGLDKGGAYALIALGLTLVFGTLGVVNFAHGALFM
                     LGCFCAVTFRYLITIQTVTIDPTRLSPWGQPVEIRTPLVQNWFGDFGAVLVDYSVPIS
                     ILLAIPVMLAIGIAMERGLIKHFYKRSHAEQILVTFGLAIVLQEIVKSFFGANPIPQP
                     IPSALRSAADIGSWLGMAPHTLTYPWWRLIYLLFSLAVIGGVFAFLHFTTFGMVVRAG
                     MADRETVELLGIDVDRRFTIVFGLAAVVAGVAGVMYTPLLPPHYHLGMDFLVLSFVVV
                     VVGGMGSLPGAVAAGFLLGILQSFASMTEVKLLIPGIDQVIIYLVAVLVLLTRPRGLM
                     GRRGIMES"
     gene            73558..74700
                     /locus_tag="VF226_17610"
     CDS             73558..74700
                     /locus_tag="VF226_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7075857.1"
                     /translation="MFSLSRKDLLSLGIFSAVVLTMPIWLTPFGAAYPGLMQKFAIYA
                     VFAIGFNILFGLTGYLSFGHAAFLGVGSYTTVWSFKLLSMDIIPAIVLAVLTAGAFSA
                     AIGYFCLRRSGIYFAILTLAFAQMCYSLAYSVLTPITNGETGLLLARNDPRIIDAALG
                     LKEASAGLPKPSLLGFEVGAYFGFYVCAFVLIVSFYISQKIFASPFGLALRGIKSNQT
                     RMNYTGFNTKPYMLAAFIISGMFAGLAGALLSVTDPLAGAERMRWTESGNVVLMTILG
                     GVGTLFGPVLGAWLIKYFENIFSAINEQMLHSFFSFLPGGLEDVFVMVLSKFVGEGWH
                     LTLGLMFVLIVVFLPGGIVEGVQRLYHQWQQRHSKPEEALSAQPAE"
     gene            74706..75479
                     /locus_tag="VF226_17615"
     CDS             74706..75479
                     /locus_tag="VF226_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075858.1"
                     /translation="MLKNSANAVLHVADVKKNFGGLQALSDIDLQFEEGQTHAIIGPN
                     GAGKSTLLNVIVGRIPPSSGAVIFDGTVLTGKAPHQINQLGISRVFQTPEIFSDLPVV
                     QNVMAAAFARRDGAFKINLFRRVEGEKEIREQAEEVLRDVGLYEQRDAHSGSLSRGDK
                     RRLELAMCLIQKPRLLLLDEPTAGMSRHDTNKTIDLLRKIKSRGMTKVIIEHDMHVVF
                     SLADRITVLAHGRIIASGQPAEIKDDPRVKEAYLGSALE"
     gene            75476..76237
                     /locus_tag="VF226_17620"
     CDS             75476..76237
                     /locus_tag="VF226_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075859.1"
                     /translation="MTVAAIERPPAVTTAIDAAGDFFAMRNVHAYYGESYIVQGVSLE
                     LGKGQILALLGRNGAGKTSTLRTIARTGQPELRAGEIWLEGKPIHTMKSFQAAQNGIQ
                     LVPEDRRIIPGMTVEENLILAQVAPGHGWDLEKVYTHFPRLAERRNQEGVTLSGGEQQ
                     MLAVARALSREVKLLLLDEPYEGLAPVIVQEIERILYGIRDLGITTIIVEQNAVAALR
                     MSDRAAILDSGEIVFTGSAKELLDNPELRDEYLAI"
     gene            76572..77717
                     /locus_tag="VF226_17625"
     CDS             76572..77717
                     /locus_tag="VF226_17625"
                     /inference="COORDINATES: protein motif:HMM:NF028293.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HEX7075860.1"
                     /translation="MASRGQQFVFSVRELSGALGDLGTLLPLMLGAIAVAGLAPAPVL
                     LGFGLFYLATAIHYRLPIPVQPMKAVVAVMLTTEISPATLASSGVLIGLTLLVLGLTG
                     WISWLARLVPQSVLAGLQLGLGLALALVSLDLMATQPVVAAVTAGSLVALLFLPRYPS
                     AIIALTVALVLGYLLGAPGIELANATSVSIPVPSLPTFDEVERALSVLVLPQLPLTFT
                     NAILLTALVAGDYFEDRAKHVTPARLSLTSGLANLLLTPFGALPMCHGAGGLAAHYRF
                     GARTGAAPLSLGLVLIAAAFLPGAGGLAVLAAIPVAGLGALLLIASGELALTKRLFDS
                     KPSCWPVIATTAAVTLWADPFWGLLAGSGAELVRMVAVRAFRREAKS"
     gene            77865..78302
                     /locus_tag="VF226_17630"
     CDS             77865..78302
                     /locus_tag="VF226_17630"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075861.1"
                     /translation="MADQARRTKMNQPKRKTELRRDAGGRAAHDVGGLDFGPIDRTEH
                     DLALWEKRVDAMLVLLTGPKKGAFKVDALRRVIEDYGQQQYDSTAYYEKWIRAIRNLL
                     VEQEVLTRAEVESKVAAVEAAMRAAGRELVGGSVPWDAMESPI"
     gene            78302..78628
                     /locus_tag="VF226_17635"
     CDS             78302..78628
                     /locus_tag="VF226_17635"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX7075862.1"
                     /translation="MEIAPRFRPGDLVRVDDRMALGHCRTPWYLRGKTGVIASVHGAF
                     HDPEKLAYHKPGLPRQVLYKVRFRQSDLWAGYAGSPRDQLEADIYEHWLIRADGEARS
                     DSHAAA"
     gene            78615..79310
                     /gene="nthA"
                     /locus_tag="VF226_17640"
     CDS             78615..79310
                     /gene="nthA"
                     /locus_tag="VF226_17640"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX7075863.1"
                     /translation="MPPHDDHDHDHDHDHAHGDGQPHRHPPQRDHDADPASPYEVLER
                     AIRELLIEKGVLTADEISAQVDLMDSRTPALGAKVVARAWVDPAYKTRLLENTRAALA
                     ELGIDIGTLADFRTVENTPDVHNVVVCTLCSCYPKLLLGIPPAWYKSLAYRSRVVVDP
                     RGVLKEFGLVLPADTEVRVHDSTADLRYLVLPRRPDGTDGWSEEQLAEIVTRDCMIGT
                     AVPEVHRTRPAAA"
     gene            79457..80863
                     /locus_tag="VF226_17645"
     CDS             79457..80863
                     /locus_tag="VF226_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075864.1"
                     /translation="MPPTANQTSGLFGIAFLQAVRRLLPQLLLAMIAAGLATFLAQSW
                     ITPRYVAEARLLPDENSAAHSDLEAHLKALKDPIRLFEVATELGLKRVPEFSGKKRGS
                     IPILSQFWSDRSGPLQRLQDQDERVLAAVYEKYSVSMAGDGTIRVSFTASDPQLAARF
                     VNRLVEVYLAGLWSPQPSASESKPTSIHDVLWAEAPEKPVFPRKGPMAILAMVLMLLL
                     GLGGIALREAIHTTPRRGRQPVEDLTAAEENNAAALGFRKLRSPIAVADHLLALPAAD
                     RGCRTMVAGEEPDIDATAEALALADALTAGGRRVVLVRWSRTGGEVSGAPAMQGTLGF
                     NDLLEGRASFEEIISRLPGSPAHAIAAGSALRDEKAALDPNLLSLVLDTLDEVYDHIV
                     VLAEHGEARVLFEVLEGRFDACISVGEAGRPAGILTASFDRFLGFEVSDIHVLRVERP
                     RQKAQPITPSPALQTRLA"
     gene            complement(80872..81174)
                     /locus_tag="VF226_17650"
     CDS             complement(80872..81174)
                     /locus_tag="VF226_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEX7075865.1"
                     /translation="MRPVGAVIAETHGESIKVNIRTRKFIGAILLMIFIAIYALLVMA
                     VAIVLQVNSSKWVEVVFYVVGGLAWVVPAALLVRWMSRPDEAGEADGAPERAGRRA"
     gene            81237..82361
                     /locus_tag="VF226_17655"
     CDS             81237..82361
                     /locus_tag="VF226_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7075866.1"
                     /translation="MPEPQVAHTAAVIREREPDTRAVRAWLYFMAALIVAMVVVGGAT
                     RLTDSGLSITEWQPLLGAIPPLSEADWHEAFAKYQQIPQYQLVNKGMSLEEFQFIYWW
                     EWAHRFLGRFIGVAFAIPFLWFWAAGRLPKGTPMKLAGVLALGAVQGFVGWFMVKSGL
                     VDRIDVSPFRLALHLIVALIILACIVWLALDLRKESGSVRLRTVTRGQKRLAATLVGL
                     VLLQSLLGAFVAGHNAGLTYNTWPLMDGRFIPEGLGTLSPWWLNIFENITTIQFNHRM
                     VAYLLAGLALTQFVWILRTADDEALTRSAGILALGIFVQAGLGIWTLLEVVPLWLGLA
                     HQAGAAIVLILAVRHAHAVMRAPAVGKAVSGSYSASPSIS"
     gene            complement(82334..83308)
                     /gene="argC"
                     /locus_tag="VF226_17660"
     CDS             complement(82334..83308)
                     /gene="argC"
                     /locus_tag="VF226_17660"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7075867.1"
                     /translation="MSSASPAQTSSSHSRKPTVFIDGEAGTTGLGIAERLRAIPAIEL
                     RSIAPERRKDLEAKRALLREVDLVVLCLPDDAARETVAMADTLPDGGPRIVDASTAHR
                     VAPGWVYGFPEMDAGQRDAIRSARRVANPGCYPTGVIALIRPLVAAGLLPQDYPLTIN
                     AVSGYSGGGKTMIEAYESGRAPAFELYGLGLEHKHLPEMQAYSLLSRRPIFVPSVANF
                     RQGMLVSVPLHLDSLPGKPTPADIEAVLSAHFAGSGLVTVVPSSDGSVRSGRLEPQAL
                     NNTDKLELRVFGNEARGQAVLVARLDNLGKGASGAAVQNIQLMLGLAE"
     gene            complement(83618..84106)
                     /gene="rpsI"
                     /locus_tag="VF226_17665"
     CDS             complement(83618..84106)
                     /gene="rpsI"
                     /locus_tag="VF226_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX7075868.1"
                     /translation="MATDTTKSLEDLGTLTGSTPEAGAAPVHVQKLDQYGRAYATGKR
                     KDAIARVWLKPGHGKITVNDKDFAQYFARPVLQMLLKQPLNAANRADEYDIVATVSGG
                     GLSGQAGAVRHGISKALTYYEPELRSVLKKGGFLTRDSRTVERKKYGRAKARRSFQFS
                     KR"
     gene            complement(84112..84573)
                     /gene="rplM"
                     /locus_tag="VF226_17670"
     CDS             complement(84112..84573)
                     /gene="rplM"
                     /locus_tag="VF226_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX7075869.1"
                     /translation="MKTYVAKPGEVEKKWVLIDAEGLVVGRLAALIATRLKGKHKPTY
                     TPHVDMGDNVIVVNAEKAVFTGNKYNDKIYYRHTGYPGGIKARTPRMIMEGKFPERVI
                     ELAVGRMLARGPLQRKLMRNLRVYKGPEHPHVAQNPEVLDVAKLNRKNVRA"
     gene            complement(84799..85254)
                     /locus_tag="VF226_17675"
     CDS             complement(84799..85254)
                     /locus_tag="VF226_17675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7075870.1"
                     /translation="MANEAAARERLTKEQVMALVDEHFPQVHEGNGRVSLEFLGPMTA
                     HVRMAHDPRSIRPGGTVSGPAMFKLADFSVYAMILGELGAQALQAVTTSMTMNFLTRP
                     QPGDLIAKARLLKLGRRLAVAEAEIYSADAPEMVAHATATYALPPEKGV"
     gene            85344..85868
                     /locus_tag="VF226_17680"
     CDS             85344..85868
                     /locus_tag="VF226_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075871.1"
                     /translation="MSPRLVQMLRSVSILAFALAFLLSPAAAEEYEFKQIKLTEKQVQ
                     SFIKAQAELNKISDKLQSDSEEPDEKVQAELEAIAKKNGFANFDELDQVAANVSMVIA
                     GIDPETGEYTDPVESIKKEIEEIKADKSIPEKEKQQMLKEMEEALETTPALEHPENIE
                     IVKKYRAEIDKALQ"
     gene            85881..86699
                     /locus_tag="VF226_17685"
     CDS             85881..86699
                     /locus_tag="VF226_17685"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7075872.1"
                     /translation="MQERVEASKTAAILLEERKGAVAILTLNRPQARNSLSLELLRAL
                     GAAIERISADAEVRAVVLAANGPAFSAGHDLKELTAHRADTDGGLAFYRTAMDTCSGV
                     MQAILRSPKPFIAAVQGTATAAGCQLVATCDLAVAADEVQFATPGVNIGLFCSTPMVA
                     LSRNVPRKRAMEMLLLGEMLSAEAAAEYGLVNRVVPRGRVMDEALAMAGVIASKSSLT
                     VAIGKEAFYRQIELPTAEAYAYAADVMVKNMMARDAAEGICAFIEKRPPEWKGC"
     gene            86704..87156
                     /locus_tag="VF226_17690"
     CDS             86704..87156
                     /locus_tag="VF226_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7075873.1"
                     /translation="MQLRLSLVTLGVADVARARRFYEAMGLTASSVSEDSVTFFQLGN
                     VVLALFGRDALAADAGVSPDGSGFAGVALAWNVASEGEVDEALSQAVASGGRLVKPGQ
                     KVFWGGYCGYFADPDGHLWEVAYNPFFPLDAAGRVSLPDDADLPSRTA"
     gene            87156..87689
                     /locus_tag="VF226_17695"
     CDS             87156..87689
                     /locus_tag="VF226_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7075874.1"
                     /translation="MAHDSYSDDLIATILRENSSIAMVGASANTSRPSYFAMKYMLGK
                     GYRVVPVNVGLAGQQILGQTVYATLADVPPPIRIVDIFRNSEAALEITREAIAQKDRL
                     GVRVVWMQLGVRNDQAAAEAEAAGLQVIMNRCPKIEYGRLSGEIGWAGVTTGRISSVR
                     PRLSPHGVQNHLIGDKA"
     gene            complement(87704..87931)
                     /locus_tag="VF226_17700"
     CDS             complement(87704..87931)
                     /locus_tag="VF226_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012556073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075875.1"
                     /translation="MTMTREDVVRVLGPVDETLIVEIIGTGASPEELEEAFAWLNADD
                     VIGRELRRQPSGKVAELCEILEREEEEPDWM"
     gene            complement(88078..89367)
                     /locus_tag="VF226_17705"
     CDS             complement(88078..89367)
                     /locus_tag="VF226_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7075876.1"
                     /translation="MLLFIMFIAMLAMILINVPIAIALAISAVIGIAATNGTDNLISV
                     GIEMYRGATSFSLIAIPMFVLAGAIMNAGGISRRLISFVSSLIGFVRGGLAMVNVGVS
                     LIFAEISGSAVADVAALGSVMIPEMKRKGYSKEFAAAVTSSSASLAIIIPPSIPMILY
                     AISAGTSIEQLFIAGFIPGVIGGLLLMVVCYVFARKYDFPVEEAFGLANLKKTFIDAI
                     PTLLLPIIILGGIFSGLATATEAAGLAVLAAILVALWYGELEFKLLKSAMLDGAVQTA
                     VVMLLVAASILMGDFLTVARVPQDLAQWIASVSSNQYVILFMLNIFFLILGFFLHSAA
                     AIILVVPIIVPLMNTVGIDPVHFGLVVTLNLAIGQQTPPVASVLITACSIAKSDVWET
                     SKVNIWFVLVLLFVLFLCTYVPAIPMFLVDLFYPNRI"
     gene            complement(89372..89884)
                     /locus_tag="VF226_17710"
     CDS             complement(89372..89884)
                     /locus_tag="VF226_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7075877.1"
                     /translation="MNSLRRTLDTLLQWTVIFLMVMLTVIVILAVFARKLGASFVWYD
                     EVSSVMLAWVTYYAAAYAALKRAHIGFDGVLLRLPLTARKIAVVIGEVLVIGFFLLLT
                     WAGLEVISVVGGDTLVSLTWMPIAVTQSVIPIGGALFIICELVSLPDYWRNVISGRSS
                     EHPEYVPEST"
     gene            complement(90012..90989)
                     /locus_tag="VF226_17715"
     CDS             complement(90012..90989)
                     /locus_tag="VF226_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7075878.1"
                     /translation="MLFRTSLAALAAVLLSASGAVAQIELKLGHVAEPGSLIAQSADE
                     FARRANEKLGDKAKITVYPSSQLGSDRDMYQKLKLNQLQLWIPSTIMSSIADEFGVFE
                     LPYIIKSREHMKLVMEKLGDNYFIPAAKAKGVLVLGQWENGFRHITNNVRPIVKPEDL
                     KGIKLRVPAGEWRIKMFKAYGANPTPMAYSEVFTALKTGVMDGQENPYPQIWGGKFYE
                     VQKYISLSGHVFTPAWLVASPTHFAKLPEDVQKSISEAAKETEEYVRELAAKLDNELL
                     EKMTSASEMKANEVDKDAFIAQSGAVYEEFGKSVKNGAEMVKEIQALAQ"
     gene            complement(91212..92390)
                     /locus_tag="VF226_17720"
     CDS             complement(91212..92390)
                     /locus_tag="VF226_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7075879.1"
                     /translation="MPRRAGRHFLQIPGPTPVPDRILRAISLPTIDHRGPEFQALGKR
                     VLEGIKSIFKTTGPVFIYPASGTGAWEASLANTLSPGDQVLMFETGHFATLWKKMAEK
                     LGLKPEFIPSDWRSGVDAAAIEARLREDKAHAIKAVCVVHNETSTGVTSNIAQVRKAI
                     DAANHPALLMVDTISSLASIDYRHDEWGVDVTVSGSQKGLMLPPGLSFTAVSEKALKA
                     SESSRLPKLFWSWAEMAPHNKNGFFPYTPGTNLLYGLAEAIDMLHEEGLERVFARHDR
                     HAEATRRAVRAWGLEILCRDPACYSSSLTAVLMPEGHDADRFRQIVLEHFDMSLGTGL
                     SKLAGKVFRIGHLGDTNDLTIIGALAGVEMGLGLAGVPHKTGGVLAAMDYLRSTSRGV
                     "
     gene            92515..>95190
                     /locus_tag="VF226_17725"
     CDS             92515..>95190
                     /locus_tag="VF226_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7075880.1"
                     /translation="MNVHVPANRALAERLRREIQGEVLFDRFSRGRYATDASIYQIEP
                     VGVVVPKTDEDVRAALEIARESGVTVLPRGGGTSQSGQTVGRSLVIDYSKYLNRLVEL
                     DPEAGTCVVEPGIVLDELNRQLKATGWWFPVDVSTASRATLGGMTGNNSCGTRSLRYG
                     IMRDNVLAIDALLPDGTPTRFAEIDPSDLGGCDAEALFRRLIALGQREAAHIAQAFPE
                     VSRRVGGYLIDALVPGNKPLNLATLLVGSEGTLALSRRIAVKLARQPKNKALGICHFP
                     KFRQAMEAAQHIVKLGPVAVEVVDRTLIELARDIAMFRPVMDTYVRGEPDALLLTEFA
                     EDDQAENLRRLDRLEELMGDLGHPDSVVKVADAAGQKAVWGVRASGLNIMMSMKSEGK
                     PVSFIEDCAVPLENLADYTERLTAVFEKYGTRGTWYAHASVGCLHVRPVLNLKLEKDA
                     NTMRAIAEEAFALVKEYKGAHSGEHGDGLVRSEFHEMMYGRKTVELFEEVKDTFDPTG
                     LMNPGKIVRSPRMNDRTLFRYKPGYHVPEFETVLDWSAYPGAGKGFQGAVEMCNNNGE
                     CRKLDGSVMCPSYRVTRNERDVTRGRANTLRLAISGQLGPGALASDEMLETVKLCVSC
                     KACRRECPTGVDMARMKIEVLAAAAKRRGVSFRDRLIAYLPRYAPYAARLAPLMNARN
                     VIPGAAWLTERLIGFAAKRKLPRWTMRPFSASAHKRGGARGESRPQGQTVGEVALFAD
                     TFNTYFEPENLHAAIEVLERLGYRVHVLEPVGGGRPVCCGRTFLSAGLADGARAEVKR
                     LLAAVKPFTERGVPIVGLEPSCLLSLRDEIPALFPGEEAAKAAECAWLFEEFLAREID
                     AGRLAGPIARSSGHLLLHGHCHQKSFNAMPAVTKVL"
BASE COUNT        16908 a        29938 c        30669 g        17675 t
ORIGIN      
        1 gccggcggag ctccttgatc gcgacgcggc ggcgcggctg ctcgggtccg atttctggta
       61 cggcggcatg ttcaatccga ccggcgggca catcaacccg ctggcgctgg ccagggggct
      121 ggccgaggcc tgcgttcggc aaggtgtcgt tctgcacgag gcatctcccg tcacgggcta
      181 cacgcgcgaa ggcgatggct ggcgcgtgcg cacggcacag ggctcggtgc atgcgcgtgc
      241 cgtcgtgctc gcgacgaatg cctatacgga tgcgatcgaa aaccgattgg agccgcgcat
      301 ttcgcgaacg gtcgtcccgg tgctgtcctg gcaaatggcg accgagccga tcggggacaa
      361 tctgcgggcg accatcgttc cgggtcgaca ggccgtttcc gacacacggg cggatctccg
      421 cttcttccgg tatgatgccc gcaatcggct gattacaggc ggcaccgtgc tcgggtcgca
      481 taatgtcgag gcgcgcgtcg tcgcgcggac gaccgagaat atcacccagg catttccgca
      541 gttggcgggt gttcgcttca cgcatgtttg gagcggatgg atcggcatca cggaggaccg
      601 tttcccgcat ttccaccgtc tcgggccgga tctttggagc tggaccggct gcaacggccg
      661 cggcgtggcg ctttcggtgg cgctcgggcg cgagctggca tcggctgtcg acggcgttcc
      721 cgccaaagag cttgcgatcc cgctgaccga accggagccc attcccttcc acccgatcgc
      781 gaggcggatc gcgccattga ttctcgcctg gtacaaacgc cgcgatctcg ctgaattgaa
      841 gctcgattga gcaacgaaag gatcaagcca tgcaggagat gcctattctg caacgccgcc
      901 gtatcgaggc gatgattttg aaacacgtct acgaggtgat ccgtgagcgc tctggtgaac
      961 aggaagcgcg agaggtcatc ggcaaggctg tgagcaatgc ggccgtcgag cacggcaagg
     1021 gattggcgga gtcgctcggc cgcacgccgg acttcgagga catgctggcc atcatgccgc
     1081 tgtggaccaa ggacgatgcg ctcgaaatcg agattctcga ggcgagcaag gataagctcg
     1141 acttcaacgt gaagcgctgc cgctattcgg aaatgtacaa ggaaatgggc ctcggccaca
     1201 tcggcgacct gctgtcctgc aaccgcgatg gggatttctg catcggctat aatcccaata
     1261 tggagctcac gcgcacgcag accatcatga aaggggcgag ccactgcgat ttccgttatc
     1321 ggatgaaatc gaaaacggag caggggcagt aaggcgatgg ccgtcgagaa ctgggtgtcg
     1381 cgtcagcttg atgagctgac ggagtttcgc cgcgatctgc atcgcaatcc tgagctgctg
     1441 tacgacgtgg agcgtacggc ttcgaaagtg gccgacgcat tgcgcgcagc cggtgtcgac
     1501 gaggtggtca ccggcatggg ccgtaccggc gtcgtgggcg tcatccgcgg caacaccgat
     1561 acgcgcggca ggatgatcgg ccttcgcgca gacatggacg cgttgcccat caccgagcag
     1621 acgggtgcgc catggacttc gacgaagccg ggtgtgatgc acgcgtgcgg gcacgacggg
     1681 cacacgacga tgctcctcgg agccgcgcgc cagctcgccg aaagccgcgc attcgacggc
     1741 acggtgattg tcatcttcca gccagcagag gagggcggcg ccggcgcgaa ggccatgata
     1801 gatgacggcc tcttcgagcg ttttccggtt caggaaatct atgggctcca caacctgccg
     1861 aatttgcccc tcggccagtt tgccatcacg ccgggtgcaa caatggcctc ggtcgatgag
     1921 attcggatca gcgtcgaggg cattggcggg cacgccgctt tcccgcacca gaccgtcgat
     1981 ccgattacgg taacagcggc gctcattcag gcggtgcaga cgatcgtctc gcgcaacgtc
     2041 gatccattga agtcggcagt cgtctcgatc accacgatca acggcggcga tgccttcaac
     2101 gtcattccgc cggtgatcac gcttaccgga accgtgcgta cgctggacga gggcattcgc
     2161 gatctggtcg agaagcggtt gcggaaagcg gtggcaggca tcgcggatgc cttcgacgcc
     2221 aaggcagagc ttaagtatgt ccgcagctat ccggtaaccg tcaataacgc tgcaaaactc
     2281 gaaaacgcgg tccgggcggc tgccgaggtg gtcggtgcgg ccaacgtgaa aaccgacctt
     2341 ccgccaacca tgggaggcga agatttcgcc ttcatgctgg aggccgttcc cggcgcattc
     2401 atttttgccg gcaatggtcc ctcggccaac ctgcatcacc cgcaatacga cttcaacgac
     2461 gagctgatcc cgttcggctg ctcgtactgg acgacgctcg tccggcagct gctggggtga
     2521 agtgagcgat tcctagtgga aacgcagcca gtagcgcctc cttcacctct cccatgggga
     2581 gaggtcggcc cgcagggccg ggtgaggggt tataacaatt gccaccggcg gaaccccctc
     2641 accccgaccc tctccccgcc ggggagaggg agatgaggcg cagctcgtca tctatatcgg
     2701 aataaaactg aaaaaaggcc tgccaaagct gtgccttttt tcgttacttc aattcgtgaa
     2761 cgcctgaata cccgtaatcg cacgtccaag gataagggcg tgaatgtcgt gcgtaccctc
     2821 gtaggtgttg acgacctcga gattgacgag gtggcgggcg acgcagaact catccgaaat
     2881 gccgttgccg ccgagcatgt cgcgagccat gcgcgcgata tcgagtgcct tgccgcagga
     2941 attgcgcttg aggatggagg tgacctcgac aggggcaacg ccctcgtcct tcatccgccc
     3001 gagccgtagg cagccctgca agccgaggct gatctcggtg accatatcgg cgagcttttt
     3061 ctgaatgagc tgattggcag ccagcggccg gccgaactgc ttgcggtcga gtacgtactg
     3121 gcgcgccgtc tcgaggcagg cctccgccgc accgagtgcc ccccaggcaa tgccgtatct
     3181 tgccgaattt aggcacgtga acgggccctt gaggccggac acgttgggca ggagattttc
     3241 ctccggcacg aacacgtcat cgaggacgat ctcgccggtg atcgaggcac gcagcccgac
     3301 tttgccatga atggctggtg cactgaggcc cttccagcct ttctccagga tgaagccgcg
     3361 aatcacgcca tcctcggtct tggcccagac gacgaataca tcggcgatgg gcgcattggt
     3421 gatccaggtc ttggcgccct tgagcgaata gccgccctcg accttgcggg cgcgggtctc
     3481 catcgagccc ggatcggagc cgtggttggg ctccgtcagc ccgaagcagc cgatccactc
     3541 acccttggcg agcttcggca ggtatttgcg ccgctgctcc tcggagccga actcgtagat
     3601 gggcagcatg acgagcgagg actggacgct catcatcgag cgatagccag agtcgacgcg
     3661 ctcgatctca cgcgcaatga gaccgtagca gacgtagctg aggccggcgc cgccgtaggt
     3721 gtcggggatc gtcgcaccga ggaggcccag ctcgcccatc tcgcgaaaga tcgacggatc
     3781 cgtcttctcg tggcggaaag cctcctgaac ccgcggcagc agcttctcct gcgcgtaggc
     3841 gcgggccgtg tcgcgcacca tgcgctcctc ctcggtgagt tgcgcctcga gcaggagcgg
     3901 atcgtcccac tggaaaaccg gcttggcggc tgcggccttc ttctcgccag tcatgtcttg
     3961 catctcgatt aagtctccga tcggtgcggc gcgtttcccg cggcgtgacc ggcgcggggt
     4021 tcgggcggaa catagcgaaa ccgccacagg ctgccagccc ccataaggga cgcagcacat
     4081 cgctagtcga cggccgggag ggccgtgtcg agcgagaata ccagacacca gtgcggctcg
     4141 aaccgggctc ccatcggcca gatgtgggcc ttgcagtcct ccagcagcac ggtctggtag
     4201 acgctaattg ctgctctgcc gcttgcccgg acgcggcggc agcgatttca agtacgcggc
     4261 cattgccgcc cgatcatcgg ccgacagctt ggacgtattg gcaaccacgt cggccatcga
     4321 ggacccgacg gagtcgtaat ccggggtgaa tccggattcc agcagatagg ctatgtcgcc
     4381 ggccgaccag gacgcaagcc cgtcctcgtg cggagtgatg ttgggcaccc agcccttgcc
     4441 ggttggattc gggccgccgg caaaacgccg ctctgggatg atgccgccca aggcgtcacg
     4501 ctggctgtgg cactcggcgc aatggccagg cccttcgacg agataggcgc cgcggttcac
     4561 gacgctgtcc tgcgccgggt ccggctcgaa cgaccttccg tcgagataga tgagcttcca
     4621 gagccccact gcccgccgca cgttgaacgg aaactgcaac tcgtggggaa tggagggctt
     4681 ggcctccgcc ggtagtgttt tgatgaaggc gaagagatcg cgcacgtcgt ccagcgtcat
     4741 gcgctggtag gacgtatacg ggaacgacgg ataaaggtgc tcgccgtcgc gaccgacacc
     4801 gcggagcatg gcattgacga acgcgatttc gctccagccg ccgatgccgt gctgcggatc
     4861 gggcgaaatg ttgggtgcgt ggaacgtccc gaaagcggtc ttgagctcaa gtccgccacc
     4921 gagccgggtc ttgtcctcct ggccgggcgt gctgtggcag gacgcgcagc cccccgcgtg
     4981 gaacatgacc tcgccattgg cgagatcggc tgttcgttgc ggaagcgcag atggtggcag
     5041 ggttgacgga atagtgagca ggtagagcgc agcgccgccg gcgatcagca gcaatgccag
     5101 cagcttgatc aggataccga gtgcggaacg tcgctttcgg gccatcgcct cagatctctc
     5161 tcgagtagcg agccttccgg gccagcaaaa ttggccctgg tatgcatggc gcccggggat
     5221 cgctcaattc ccgagcgcca agcatacata tatgcgagaa ttgggcggtt agtcgggcct
     5281 gcgatactgc ttgtggcagc cgccgcagtt gctcatgacc tttggccatt cggctttgaa
     5341 cgacgcctcg tccttgatgc ttgctgctgc ggctttggca tcggccgcga gcttctcgaa
     5401 acgtgcaagg aaatcgtcct gcttttccca gatgattgga agtgcggctg tctcgccgtt
     5461 gttggagccc ttcggccaga gcttcttcag atcggaagcc ttctcctggt agacggcaag
     5521 tgactggcga atcttgtcga gatcgaattt cgcttcacct ttgatgacgg cgcctggctc
     5581 tttggctgcg cccgccatgg ccttcatggc ctgctggcgc tgtttgatga cgtctgtctc
     5641 ggcgttcgcc gccgtataga tggtcgcccc gacagccaga gccacagcga ccgagagaat
     5701 gcgcatcatc aacgaacctc cattcagaag tgccgggcgg agattgcagg gggccgccca
     5761 caagacggcg aaggctgtca gcgcttgccg ccttagtcaa ttgaagggcg cattcgaggc
     5821 tctcacctta ccgtgagcag tcctattcac ttgatcggtg gcgcggattg ggtgctgatg
     5881 ggaaggctca ccaattgcag ggcacttctt tcaccacatg acactcaccg ccagcaacac
     5941 cggcccggac agggtgtttc gtctgcggga cgggcgccgg atcagctatg cggagtttgg
     6001 cgctcccgac ggccgcccag tcctggcgct gcatggcacg ccggcgtcgc ggctcatgta
     6061 cgcgattgcc gcctcgagcg ctgcgcggct gggactgcgg ctcatcgcgc cggaccgatg
     6121 gggttacgcc ggcaccgatc cgcatcctga gccctcgctc tccgcatggg cgcaggacgc
     6181 ggccgagctg gctgacggcc tcggccttga tcgtttttcc atcatcggca tatcgggcgg
     6241 atcaccttac gcggcgggga cggcagccga gctcggcagc cgggtgatcg ccatggcgct
     6301 ggcggtaccc gtggggccgc tcgtgtcgaa tggctccaag aggcggcttc cgctgcttca
     6361 gcgcctctcc ttcttctggg ttggtcagcg tccctcactg acgaagtcgg tgttcaaagc
     6421 ctatggccgg ttcctcaacc ggtggtcccg gcgtgccgtc tctctcgcca tgataggcca
     6481 gtcgcgcttc gaccgtgagc tgctccgcaa gccggccatc aacgattact ttggcgagat
     6541 cagccgcgag ggactgcggg cggggggcgc cgggccggcg atagatcttc agttgttctc
     6601 gagtgagtgg cacgtcccgc tcgatagaat ttcggcaaag acgcggatct ggattggcaa
     6661 ggacgataag ctggtcccgc tcgccgcggc ccgcgagctt gcccgcgcaa ttccgggagc
     6721 gatcctgaca gagctcgacg acaggggtca cttctggatc gcagaagagt acgggcaggt
     6781 tctcgactgg ctcgcggcgg caggccgcga cgaaggtgag gtcaggcagt agccctgttg
     6841 gacagcgcga accaccactc gcggatgccg ccgtgcggct ttgccgcggc gatgtcccgt
     6901 tgctcaaggc gctgggttac gggctctgca gggcgccacg gttgcggatc gcttttgggc
     6961 gccggtgcgg ggacgagcag gtcttccgag ttcgccatgg cgtatccggc tgcctctgca
     7021 gctcttatga gtttgtcgat acccattgga accacgcccc tgctcaccgc ttggaccaag
     7081 cagataacac cgctttaata tcaaagttcc atgacagtta tgatgaggct caaataacgc
     7141 acgtcaagtt aattcttcag tggccctacc atcggcttag tctattgcac tgcactggca
     7201 gggatgctgc agtgcaggca ggctttcttt aggttttgaa gcttttggca ggctgtacgg
     7261 ccttggaccg gtagccgtgc gaacccggcg ccgagcgggg cgggcgccgc aaagagggca
     7321 ccggctgatc gatacggcat gagctcagag cgcgccatgc cgcgggccgc gatcccgcaa
     7381 acggccttcc tcccttacga atcccccctt gcgctcagga tcaggcggcg cgaacataac
     7441 gatcctgccg cgctcctctc gaggcgcggg gcgcgcggtg cgcgtcctga cgcctacaga
     7501 gaggaggatc ccatggggca aaccgcaggc tcgcaaggcc gctctgcccg gtgcatcgca
     7561 ctggtcggac cctatctcag cggcaaaacg acactactgg aagccatact cgcccgcagc
     7621 ggagcgattt cgcgacaagg ccgcgtcacg gaaggatcga cggtcggcga caacactccc
     7681 gaggcgcgcg ctcatggcat gagcgtcgag ctcaatgtcg cagaggtcga gtacctcggc
     7741 gaccagttca cgttcatcga ctgcccaggc tcgatcgaat tccagcatga aagctccgcc
     7801 gcgctgaccg cctgcgacgc agccgtggtc gtctgcgagc ccgatcccaa gcgcgtgccg
     7861 gcgctgcagc tcatcctcaa gcagctcgag gaccgcgcca ttccccacct gctgttcctc
     7921 aacaagatcg atacgttcga cacgagggtc cgcgacatca ttccactgct gcaacccgcg
     7981 agcgccacgc cgctcgtgct ccggcagatt cccatttggg agaacgggat cgcgatcggc
     8041 ttcgtcgatc tcgcgctcga gcgcgctttc gtctatcgcg agcatgcgcc gtccgaaatc
     8101 gtcgagatgc cgcaaggcgt gcaggagcgc gagcaggaag cccgcttcca catgctcgag
     8161 caactcgcgg actatgacga cgagctgatg gagcagcttt tgtcggatat gcagccgccg
     8221 cgcgacaagg tcttcgacga cctcgccaag gagctgcggg aagggcgcat ctgcccggtg
     8281 ctgctcggct cggcagagcg tggaaacgga attttcaggc tgctcaaagc gctgcgccac
     8341 gaatcgccat tcgtcgagcg gacggccgag cggctcggct tctccggcgc gcagtctgcg
     8401 gcctacgtgt tcaagaccat ctacacggcc catggcggca agctctcgtt tgcgcgcgtg
     8461 ctggccgggg aaattccgga cgggtcgacc gtggtcggtc ccgacggcga ggatcgcatc
     8521 gccggcctgt tcactgtcat gggccaggag acccgtaagc gccagaatgc cgtggctggg
     8581 gagacggtcg ccctcgggcg tctcgaccac atcaagaccg gcgacaccat cagcctcgat
     8641 aagaacggcg tccagcagat cgagacccct caggcgcccc agcccgtcta tggcatcgcg
     8701 ctcgcgctga aggatcggaa ggacgaggtc aagctctcga cggccctcgg caagctgatc
     8761 gaggaggacg ggtcgctgtc ggttgagcaa aaccccgata cgcaccaact cgtcctcctc
     8821 gggcagggcg agatgcacct tcgggtggca ctcgagcgtc tcaaccgaaa atatggcgtg
     8881 gcagtcgaca gccgacggcg cctcgtgccc tataaggaga cgattcgcaa gccgaccacg
     8941 gtgagggggc ggcacaagaa gcagtccggc gggcacggac agttcggcga cgtggtgctc
     9001 gacatcaagc cgttgccacg cgggagtggc ttccaattct ccgagaccat cacgggcggc
     9061 gtagtgcccc gacagttcat accctccgtc gagatcggcg tgcaggacta tctgaaacag
     9121 gggccgctcg gcttccccgt agtcgacatc gcggtgacgc tgacggatgg cagctatcat
     9181 acggtcgact cctcggacat ggccttccgc caggctgggc gcattgccat ggcagagggc
     9241 ctgccccagt gcagcccggt gctgctcgag ccgatcatgg cggtggagat tgcgatcccc
     9301 tcggacgcga cggcgaagat caacgccctg atttcgcagc ggcgcgggca gatcctcggg
     9361 ttcgacagcc gcgagggctg gccgggctgg gatgtggtgc aggcgcagat cccggcggcc
     9421 gagatggacg atctgatcgt agagctgcgt tcggcgacgg ccggtgtcgg atcgttcacc
     9481 gcccggttcg atcatctggc cgaactgacc ggccggctgg ccgaacaggt gctggcgcag
     9541 cagaaagcgg ctgccgagta aggacgtgcg tgtagggcga gggcgcggcc ctctgcagag
     9601 tcgctgcgcc ttcttgcaaa cgtgggcgcg aatgagcatg gttcacccgt catgccgcgc
     9661 ccttcgaccg atcgacagct ccatttgtct gccccggagt tccgcaggct cgcccgcgaa
     9721 cgcctctatc ccactccaag cgaagccatt ttcgaccctc gtaccgggcg gagctgggcc
     9781 cggagcgact tcgacctgaa cccggagctc ttggcagacc tcgctcttat gaagccgccc
     9841 cgtccggcgg ccgtgctggt gcccatcgtg gcccgcgagg agctgacggt gctactgacc
     9901 gtccgcacgg acaacttgcc gactcacgcc ggccagatcg cgtttcctgg cggtaaggtc
     9961 gaacccgtgg attccggacc gatcgctacg gccatccggg aggcgcagga ggaaatcggc
    10021 ctcagcgccc gctacgtcga gccgctcggg tatctggaca gctatcgctc ggggagcggc
    10081 ttccacatca tgcctgtcgt ggctttggtc gaaccttcgt tctcgctgaa gcccgatcct
    10141 gcggaggtcg ccgatacgtt cgaagtcccg ctgtccttcc tgatgaatgt ggccaaccac
    10201 cagcagcatt cgcgcgaatg gctcggccgc cagcgctact tctatgcaat tccgttcgga
    10261 gatcgctata tctggggcgc gactgccgga atgataaaga acatgcacga aaggcttttc
    10321 ggcgaatgat ccgtctcatc atcgagaaca cgctgctgtt tctgctgccg acggccatct
    10381 atgtcgcgta tgtgctgctg aggcgtcgga tcgagggcgg gaactcgaat gtcaatgtgc
    10441 tcgacgatgc accattgatc tggctggtgg cggcgggtgc tgcgctggtg atcgcgacct
    10501 tgatcatctt tggttcggtt tccggcggga aaccggggca gacctatcat ccccccgttt
    10561 accgggacgg caggatcgag cccggcgaga tcaagtgagg gctcgatgac ggcgggaggc
    10621 atcgagctgc cgcgcctcga ccaagccgag tggctgacaa atccgaagac gcaggcggtc
    10681 tttcgtgcga tcgaagcggg cggctatccc gcgcgggccg tcggcggcgt ggtgcggaac
    10741 gcgctgctcg gcgagcccgt aggtgacgtc gatatcgcaa cgccggcgcc ccccgaggag
    10801 gtgatgcggc tcgcagatgc cgcgggcctg aaggctgtgg cgacgggtct gcagcacggc
    10861 accgtgacga tcgtatccga ccatacgcct tacgaggtga cgacgctccg ggaggacgtg
    10921 gagaccttcg ggcgtcacgc ccgggtcgct ttcaccgacg actgggctgc ggatgcgcgt
    10981 cgccgggact tcacgatcaa cgcgctctat tgcgacgcac ggggcaacat ccacgatccg
    11041 ctgggcggct ttcccgatct gcttgcccgg cgcgtccggt tcatcggcga tcccgccgag
    11101 cgcattcggg aggattacct gcgcatcctg cgcttctttc ggttgactgc cgtttacgcg
    11161 gcggggcctc cagatgaaga aggtctggca gcggcggtgc gcgagcgcca tgggctgacg
    11221 cgactttcca gcgagcgaat ccaccaggag ctggtgcgtc tgctggcagc ccggcgcgca
    11281 tcggagctca tcggcgtgat gcgagactac ggtctcttga ccatcgtcct gcccgttgcg
    11341 cctcgtcccg agctaatgcg gcggctgatc gagctcgagc aggcgctggg gcgtgatgcg
    11401 agcgcgatgc tgcggctggc ggccctcggt gtcgaggtct tcgaggacgc ggtcaggttg
    11461 cgcgaccggc tgcggctgtc gactgctgag ttcgagaccc tcgttcgtgc cgccgacgca
    11521 ccgcgcaggg ggccccatcc gggcgacgat gtgcaccagg cgaaggtcag tctctaccgc
    11581 ttcggcgcaa aagcaatgcg cgagcaactg ctgatcaact gggcacggtc cgccgcaccg
    11641 cccgatgatc cgcagtcacg cagcctctat ggtctggcgg atgcctggca gccgccgacg
    11701 tttccgctca gtggtgccga tgtgctcgcc gccggcgttc cgcccggacc cagggtcggc
    11761 gagatccttg gagagctcga gaaccgttgg ctcgacagcg atttcagatt ggatcgggag
    11821 cagcttcgcg cggagctcct gcggctcggg tccgagggcg attgaaggag ctcgaaagct
    11881 ccgaaggcct tttttgagtc agctcaaacg aaaaaggcgg gccataggac ccgccgcgcg
    11941 aacatttctg aagaattgct caattggcag ctggagcctt tggcgggttc gcggtggcca
    12001 cctcggtggc tgcctgagca gattgactgc tcgccgggga agccgtcgtg accgtggcat
    12061 ccgcagcagg ggccgtagcc gtctgcatgt ggttatgcgc cgggcaagcc atcaggcttg
    12121 ggccgaatgc gatggcgaga atggcagcaa gaaccagagt cttcagtgga aacatcgaac
    12181 gtcctcctct gatcacctct cgctgagcgg gtatcctcga taggtctgca ccgactttgg
    12241 accaattcgc tgtccgctgg cgatcacagt ttggtgtcgg tcggattatt gcaggggaaa
    12301 acctgaactc caggtgaacg gcctgcctga tctggcccgc aagatgagaa gcgccccggt
    12361 ccagcaggga ccggggcgcc acattctcga gatgcctgcg gtgcgggatc agaagtccat
    12421 gccgcccatg ccggcgcccg ccggcgtagc cggcgccgat tcctttttcg gccgctcggc
    12481 gaccatcgct tcggtggtga tgagcagacc ggcgaccgaa gccgcatcct gcaacgcatg
    12541 gcgtacgacc ttcgtcggat cgatgacgcc ctggctcatg agatcgccgt actcattggt
    12601 ctgcgcgttg tagccataag agtacttgtc gttctcgaga atcttgccga cgattacggc
    12661 accgtcctcg ccggcattct cgaagatttg ccgggctgga gcggccaaag ccttgcgcac
    12721 gatattgatg ccgaacttct ggtcctcgtt ttcggcattg agctcatcga ggatcttcgc
    12781 ggcgcgcagg agagccacgc cgccgcccgg aacgatgcct tcctcgacgg cagcgcgcgt
    12841 cgcgttcagt gcgtcatcga cgcggtcctt gcgctccttc acctcgacct cggtggcgcc
    12901 gccgaccttg atcacggcca cgccgccggc gagcttcgct aggcgttcct gcagcttctc
    12961 gcgatcgtaa tcggaggtgg tttcctcgat ttgcaccttg atctgctggg ttcgcgcccg
    13021 gatgtcctcc ttcttgccgg cgccgtcgac aatggtcgtg ttttccttgt caatggtaac
    13081 cctcttggcg cggcccagca tgtcgagtgt gacgttctcg agcttgatgc cgagatcctc
    13141 ggagatgacg gttccgccgg tcaggatcgc gatgtcctcg agcatggcct tgcggcgatc
    13201 gccgaaacca ggggctttga cggctgcgac cttcagtcca ccgcgcaact tgttgacgac
    13261 gagtgtcgcc agagcctctc cttcgacatc ctccgcgatg atgagcagcg gccgggagga
    13321 ttgcaccaca gcctcgagca tgggcagcat cgcctggagc gaagacagtt tcttctcgtg
    13381 aatgagaatg taggggtctt ccagctcgac cgtcattttc tcggggttgg tgatgaaata
    13441 cggcgaaata tagccgcggt cgaactgcat gccctcgacg acctcgagct ccgtttcgag
    13501 gctcttggcc tcctcgacgg taatcacacc ctcgttgccg accttgcgca tcgcctcggc
    13561 gataatctgt ccgatctcgg catctccgtt ggcggaaatc gtgccgactt gcgcgatctc
    13621 ctcattggag gtgattttct tcgacttcga gcggagctcg tcgaccacac ggttcacggc
    13681 gagatcgaca ccgcgcttca ggtccatggg attggcgccg gccgcaaccg acttggcgcc
    13741 ctcgcgtacg atcgcgtgcg ccagcaccgt cgccgtcgtg gtgccgtcac ctgcctgatc
    13801 ggcggtcttc gaggcaacct cgcgcaccat ctgcgcgccc atgttctcga atttgtcctc
    13861 gagctcgatc tctttggcaa ccgtaacacc gtccttgctg atgcgcggcg caccgaagga
    13921 cttctcgatg acgacgttgc gacccttggg gccaagagtg actctcacgg cattcgccag
    13981 gatatccacc ccgcgcagca gacgctcacg ggcttcttgc gcgaatttca cttcctttgc
    14041 tggcatggaa gcggctcctt ggttaggggg acaggaccat caactcgata tcgaaatcca
    14101 cgcctggcgt ttgatcgccg ggggggcaaa agctttcttg ccgagagggc gaaagctcag
    14161 gcggcagccc gggccgaacc ctcaccttcg agaatgccga ggacgtcgct ctccttcatg
    14221 atcagcaggt cctcgccatc gatcttcact tcggtgccgg accacttgcc gaacaaaacg
    14281 cgatcgccga cgcgaatgtc caagggcacg attttcccgc tttcgtcccg cttaccgggg
    14341 ccaacggcaa ggacttctcc ctgcatcggc ttttccttcg cggtatcggg gatgatgatg
    14401 ccgcccgctg ttttgttttc ttcttcgatg cggcgaacaa gaacacggtc atgtagaggg
    14461 cgaaacttca tgtctccaac ctcattttgc ccgagttagg ttgcgcttgc ttgccaaggt
    14521 ccctgccggg aaactgtgcc cagaagtaat gggggctgtt aaagcgttcc caggggtata
    14581 tgttgcgaca cattagcact gtcaagggga gagtgctagt cgacccgagg tgcaagagcg
    14641 cctttagcga gcgggcacga gtaatccgct gtgatgaggt ttgatatact cggctgcgcc
    14701 agagataaac gcagaacagt tgccttctat tgccggtttg gcggatatcg atcggcaaaa
    14761 aagctaaggg cgctcgtgcc tgacacaagc gcccttggca gttgaggtat ttgcagctct
    14821 tcagcgcact gcaaagtctt ccagcttggc acctttgtcg agcttcgccg tcagccaccg
    14881 cggcttacgg ccacggcccg tccaagtctc cgagcggtta tccggattga cgtacttcgc
    14941 agcgacctgc cgccccttgg cagccggtgc acgtccgccg aacagctcgc tcaccgaaaa
    15001 tccagcgttc tgaacgattg cttcgatctt ctgctttacc tcggtgcgct cgcgctcgcg
    15061 agcgtttgaa atcgccttct cgagcttggc ctcgagatca agaagctcct tcagcgacat
    15121 cttatcgaga tttacgctgg ccattcgctt cggttccttt cgtcttgcgg tcctagtcac
    15181 gttacctcac atgttgggca gttgactggc cccccaactt cgcattgtga aaccagccgt
    15241 ttataggatc acgtcggcta aataaagaga gattcggtcg cagttgacta aaaaacattc
    15301 tcccgcgaac aaggagaggg aaatccattg ccggttaaca cttcgctaaa ccaattgact
    15361 aagccaatcc acaccatggc tataaggcaa cggagcgatt gggtgactca tacgtctgcc
    15421 ctttggttct agcgaaaatc tgtcgatacc ttcagatcag gtacttgtcc atattgccga
    15481 ccttccaaca cgtcaaccgg ttcccggcct tcgaaggaat acccgaccag tgtcaagccg
    15541 gcagatgcaa tcaacggctt tgggaaggcg ctggggattg ctgacataag cgcaggcgac
    15601 agcccatagg agcaggcgtt caatattccc gctgtttgtg ggcttcggca gcagatattg
    15661 tgccgcgact gatcgcgagt cgaggacaag aggggaacat gcttcagcgc tatttcgacc
    15721 tggacgggca cggaaccaac gtacgcaccg agatcgtggc aggcgtcacg accttcatga
    15781 cgatggccta tatcattttc gtcaacccgc agatcctcgc cgatgccggt atggatcgcg
    15841 gggcggtatt cgtggccacc tgccttgcgg cggcattggc gtccgccttc atgggtctct
    15901 atgccaatta tcccatagcg cttgcgcccg gcatggggct caacgcctat ttcacgtacg
    15961 gcgttgttct gggcttgggg tatagctggc aaactgccct cggcgccgta ttcctgtccg
    16021 gaatcctgtt tctggtggtc agcctctcgc gtatacgcga atgggtgatc gacagcatac
    16081 cgctttcgct gaagctcgcc atcgccgccg gtatcggcat gttcctcgcc attatcggcc
    16141 tcaaaagtgc cggcatcatt caggcgagcc cggcaacgct cgtcaccctg ggcgagctga
    16201 aaaccttgcc ggtcgcgctc gcggccgcag gctttcttct tatggttgcg ctcgatgccc
    16261 gccgtgtgcc cggcgccatc atcatcggta ttctggcagt gactgcggca ggtattgcgc
    16321 tcggtaccaa tcagtttggc gggatcattt cactgcctcc atcgatcgac ccggtgttcc
    16381 tgaaactcga catcgtcgga gccctcgagg tcgggtttgt tgccatcgtt ttcacattct
    16441 tttttgtcga cgtgttcgac aacaccggta cgctcgtcgg tctcgcgcac aaggcgggcc
    16501 tcgtcggccc ggatggaaag cttcctcggg tgagcaaggc actgacggtc gactcgacgg
    16561 ccgcaatggc cggcgccgtc ctcggcacct cgactaccac cagctatatc gaaagcgcgg
    16621 cgggcgtgaa tgctggaggg cgcacaggac tcgtggcagt gactgttgcc gtgctgttcc
    16681 tggcggcgct gttcttcgcg ccgctggccg gatccattcc ggccttcgcg acggcgccgg
    16741 cgctcgttta tgtcgcttgc atgatgatgt ccgccgtaaa ggagattgat ttctccgatt
    16801 cgacggagta cgcgccggcc gttatcacga tcgccgcgat gccgttgacc ttctcgatcg
    16861 cacacggcat cgctttcggg tttatcgcat atgccggcat aaagctgctg gcgggtcggc
    16921 tcagcgatgt caggccggcc gtggcgatcc tggctgctct gttcgtggcg aaattcgcat
    16981 tcctgtaagt tggcgcgccg gcagggcgac gagggatgaa gggtgagact ttcgcccaac
    17041 atctctcgcg gttcgggaaa gatccacaaa ggagcatgat ctgcggaggg agcccggctt
    17101 ctccaattct ctgacgaaat cgcgcagagg cgcgccagct cctccgcgct gatccggagc
    17161 tccgaagcgg agcgctcatt cctggagcgc ttcgccttgg tcttcagtga tgcagatacc
    17221 gaaaagccgg ttcaaggcgc gcgttagcgc ccttcgcgcc accaggccat cgcgatgagg
    17281 acgagcaggg cggccagagc aaggaaaccg gtgaacatcg gcgtcagctt tacgccgcgt
    17341 gtcacgtagg cctcacggtc cttgaggccc atccagcccg accctgcgag cacgcgggcc
    17401 gaggacagca tggtcacgcg ggggacgtcg acggaccccg ggtctctgct cgagaacagt
    17461 cccccgcctc gcgtccagaa cacgccgccg ccggtcgctt cggagatggg cctcatcttc
    17521 tggtccgtcg ccgtcacctc agacatctcg cgcgggtctg cggcgccggc atgggcgagc
    17581 gccgtcagca ttcctgtcgg agactgcgtc tgcatcttgt aaaggcccgg ctcgcgcaca
    17641 tcgatcgtgg cgcgccaaag cccaggtccg gcctcgtcca gggtgacctc tgacgtctcg
    17701 ccgctcggtg cgagcacgct cacggacgga acggtgggct ccatggaccg gcgctcgatc
    17761 gtgagcttca gcccgcgagc agaggccgtc agccgttcct cctccaaatc cggctctttc
    17821 atcagccagt gcgacagcct ccgcagcaga tcggtatgag gtccgccgcc ttcgaaaccg
    17881 cgcgcccaca accacgcatg atccgaggtg aggagcgcaa cacgaccctt gcccttgcgg
    17941 tcgagcacga gcagcggctt gtcctcggct cccttcatga tgattcggcc gcgctcgggg
    18001 cgcacatcga cctggcggaa ccaccgcccc caagtcggat gctccccatc tccgccgccg
    18061 ggcaggccgc gcgtgaccgg gtgcttgatg ccgtcttcgg tcagcttggc ctggaacggc
    18121 tgctccagca cacgccccgt cggcgttgcg ggcagcaccg gcgcaagcgg tgtgcgcacc
    18181 aggctcatct ggccggcgta gtcatcgcct gcggccacca gcagtgcccc gccgtacgtt
    18241 tcgacgtagc gggcgatgtt gtcgtagtag aggagctgca agatcccgcg atgctgatag
    18301 cgatcgaaga tgatcaggtc gaaatcgcgg attttctggc tgaacagctc ccgcgtcggg
    18361 aaggcgatca gcgacagctg gttgatcggc gttccgtcct gcttctctgg cgggcgcagg
    18421 atggtgaaat ggacgaggtc gacggccgcg tcggacttga gcaggttgcg ccacgtccgt
    18481 tcgccggcat gcggctcgcc ggaaaccagc agcacgcgca ggttttcgcg cacaccctcg
    18541 gcggagatca cgatgcgatt gttggccggg gtcagctcac ccggcgccgc ttcgagctcg
    18601 acttccagga tattctggcc ggcatgcggg aattgcatgg ggatgcgaac ccgctggttg
    18661 atctccgctc gccggatctc gtcggcgcgg ccctcgcggc gcaccctgag cgtcaccggc
    18721 ccgcgcgaac tctgggcttt tccggtctcc cgtacggcca gctcgacgtc gcgggtctgt
    18781 ccgacgatgg cgtatttggg cgcgaccagc acctcgatgc gccgatcgaa ctcgtcgggc
    18841 tggccggtca gcagcgtatg gaccggagca tcgaagccga gtgccgcggc atccttcggg
    18901 acgtcatgca cctgcccgtc tgtcaccatg atgacgccgg caagtcgatc cggcggcgtg
    18961 ttggcgagcg ccttgttgag ctcctcgaac aggtttgtgc ccgaagtcct ttccccgtcg
    19021 ggccgcgccg ccgtgatcca gcgcacctct agaccgggga tctggccgag ctttgcttcg
    19081 aggtccgcgc gtatcgcctt ggcctgctcc ggccggcctg caagggtatt gctcgtgctc
    19141 tcgtccatta ggacgagggc gatattgccc aggtgttcgc gctcctcctg gcgaagcgtg
    19201 gggttcataa gggccaggac gagcgcggcc agcgccagcg cgcgcaacag ggttccccgc
    19261 gaccggcgga atgtgagcag gccgacgagg agcaaggcga cgatcgcgcc tgcgataatg
    19321 aagggccacg gcagtatggg cgcgaagtcg atggaccagt tcatctaaat cttttccgat
    19381 gtagacggaa ccatgccagc gctcgcgccc aatctccctc tcccccaatg gggaaagaat
    19441 tgggtgaagg cgttccgcca gaggcaaccg tcacaaccct caccccgcgc cttgcgccgg
    19501 attttcccgg cgggagaggt ggagcaggag cgtcgagccg ccgcttcgag ctgatttgca
    19561 gactcatcca ggaccttgaa ccttattgtc cgagccgctc gagcagagcg ggcacgtgca
    19621 cctgatcggc cttgtagttg ccggtcagag cgtacatcac gatgttgatt ccggtgcggt
    19681 aggccatctc gcgctggctc tcgccgccag ggaccgtcgg gtagaggggc cggccccgat
    19741 catccatcgc ccatgccgcc gcgaagtcgt tggaggtcac caggatcgac gacaccccgt
    19801 cggcccgccg agccttgcgc ccttcatcga ggctgtcgtt catgccggcc tcgacccaga
    19861 gctggccgcc atcccagcgg cccgggaacg agcgcagaag gtagaaggat ttcgtgagca
    19921 cgtggtcttc gggcaccggc tcgagccgcg ggatgtccag tcgcccgagc agccgctgca
    19981 atgctgttcc gccgtcgctg ccacgcatgc tgaagccgaa agggacaccc tggccgaagt
    20041 cgcgggtatc gaagatgatc attccgccct gcttcatgta ggcgtcgatc ttggccagcg
    20101 tctcctcggg cagcgacctg gcattttcgg gtaccggcca gtagaggatc gggaagaacg
    20161 cgatctcgtc ggtggtgatg ttgaccccga tgggatcgcc gggctcgacg gcggtacgca
    20221 acgagaggac cttgcccaaa cctgaaaggc cggcacggct gatttcatcc gtctccggat
    20281 cacctgtcag cacgtagccg aaggttacct tgctcgtagc tcggagcgcg agggcgtcgt
    20341 tgtttcgggt cggcggcgat ggtgcggccg aaccggacac cggctgcgct tccgcgctgc
    20401 ccgttgcacc ctggatcagc agtattgcaa gagcaatggc tcctgctgcg cgtgcggcct
    20461 gccgcaccgg gcgcaaggcc agcccgccga gctgcaacag aatgactgcg atgatgtccg
    20521 cgaacagcag tccgagggcg agtgcgagca gccaaggctt catcggcgtt gcgacctgac
    20581 cctcatagac gcggtgctgg gcggacgccg gcaatggcgg caggggcttg agctgggtgt
    20641 cgggatgaat cagattgagc gccctcggag caccctgggc accatagtag ccgggtggat
    20701 gctcggcgtc aggcgtaacg ctgtcgagct cggcgatgga aatggcctgc gccatcggag
    20761 gaggtggttt caacacaccc cacccgtcca gaagctgcac cggcgggagg acgctcgcat
    20821 cgttcgcagt cgtgcccgct gcacccgtat cggcagcagt cgcctctccg ccgccgccca
    20881 gccgaccgag ggtgctcacc cggcgcaaca tctccacgaa caaaccggag agcggcaggt
    20941 tggaccagtc ggagttggcg gtcacatgga agaggacgag ctggccgtca cccagcgacc
    21001 gtgccgtcac cagcggcgtg ccgtccgcca accgcgccca gacgttgacg tcgggactaa
    21061 gctgggtcgg gtcggccagc acttggcggt tgaccatgac atccttggga acgtccagcc
    21121 ccgcgaagat gctgtcgtct gaaaacgggg ccagcggctg tggcgtcgac caggagagcg
    21181 cccctcccag cgtgcggccg cccgcgcgca gccccaccgg aagcagatgg tcgccaccgg
    21241 cttcgagccg tggtccggca aagcgcacca gcacgccgcc tttcttcacc cagtcttcga
    21301 gatgcttcgc gacctcgccc gtgagtgtgc cgatatcggc gagcatcagg atcgacgcat
    21361 ggcgctgcag caccgtttcg acgcccgaca tcaagttggc accgcctggc tcgacgagct
    21421 cggagaaggg cgcgagggcg cgctcgatgt agtagagcgg cccgaggagc ggctgagcct
    21481 gctcgctgct ctcgcccgag agaaggccta tccggttcca ctgcgagcgg gcatcgagca
    21541 ggtgcacggc ccccgctgaa ggctcgcccg cgatctcgac ccgggtgacc tggttgcgca
    21601 gctcgagcgg cagctcgaac gtcgccttgg ccgttgtctc gcccggcgcg aaccggaagg
    21661 gtgcttcgcc gagccgctgc ccacgggcgg agaacgcatg cagcacgccc tcccgggcac
    21721 cgccgccggt ccggacaacg gtggcctcga gccggccgcc cctgccgaca ttcgtcgcga
    21781 cgcccatcgg ctcgtcgccg acactggttt cgatgacgct gaggctggcg gttccgagcg
    21841 cagacagctt ctgcaggaag gcgctcgtcg cctctccgtg atcgatgccg tccgtcagcc
    21901 aaacgatgct gacctgatcc tgtccggcga gtaccggttc cagagctgac acggcggcct
    21961 gccggtcagg ggcgaacggc tgcggcttga gcgccgcagc ggccgtgcgc gccacattgg
    22021 gcgcctcgat gcgcgccgtc ggcatccggc cgctgaacgc ggtgggcagc acgacaaccg
    22081 gacggttctg gctctctgcc tcgtcgatga gctgatcgat catgcgggtg cgcaatggcc
    22141 agcgcgaggc ggaagcccag ccattgtcca cgacgatggc caccggcccg ctgccggtga
    22201 ggcccgcctg tcggttcgga ttgaggaccg gttcggccag cgcaaggatg accagagccg
    22261 ccgcgagcat tcggatcaac atcagccacc agggcgtctg ggccggtgtc ttctcgcggt
    22321 tctcgaggcc gaccaggata cgcgtggcgg gaaacgggac ctggcgcggc cgcggcggca
    22381 ccgtgcgcag cagccaatag atgatgggta gtgccgcaag ccctgccagc agccacggat
    22441 tcaggaatgc gagcgcgccc aggctcatcc ggcaactccc gcgcctgcgg cggccgccca
    22501 gcggtaatcg tggccactgt tctgcaggcg catgatgagg gtcagcagcg gctcggccgc
    22561 gggccgatcc gtgtgatgca cgaggaacga ccagccgagt cgcgccgcga gcgactcgag
    22621 ggccgcccga tgggccctca acctcgcctg atactcgggc cgcagcgcct cgacgcgatc
    22681 tgcgagccag cgctcgctgc cttccatgcc gagaaactcg gtgcggccct gatacggcag
    22741 ggtctcctcg gcgggatcca ggacctgaac catgtggccg gtgacgccgt cgccggcgat
    22801 atgggtcagg cgttcggaga tggtctcgat gggctcgagg aaatcactga acagcagcag
    22861 gcccgaaaaa cggttcagcc gctccgcagg cggcaggctg gcctccaggg cttcagagcc
    22921 gatgttttgg gccagagctt ccgccagctt ggaagtcgcc tttcggcttg cggttggccg
    22981 cgacagtccg aggagcgcca cgcgctcgcc gcctcgcacg agcagttcgg ccgcggcgag
    23041 cgtcagaacg accgcacggt ccgccttcgc gtctgctgca agctggctgc ggaaatacat
    23101 cgacggcgaa aggtccggcc agagccacaa cgtgtgagcc gcctcccatt cacgctcacg
    23161 cacgtaaaga tggtcggagc tcgcggaacg gcgccagtcg atctgggccg ccgcatcgcc
    23221 ggactggaac tgccggaact gccagaaggt ttcgcccggc cccggccgcc gccggccatg
    23281 aattccatgc gcaaccgtac tcgccacccg gatcgcctcc accagaaggt cggggagccg
    23341 gtcggcaagc gcgtgcgctt cgccttcgag cgcgagcatc ggcgcgccgc gacttgaatt
    23401 ggaagacgat ctagccgctg cgggcttggc cacaacttac tccagtaccg aggcgaggcg
    23461 tgcgattatg ccgccgatct cctggccttc ggtgcgcgcg acgaagctca gcgccatgcg
    23521 gtgcttcagc acaggctcgg cgagcgcgat gacatcgtcg accgacgggg cgagccggcc
    23581 ctggagcagg gctctggcgc ggactgcgag catcagggcc tggctggcgc gtgggcccgg
    23641 gccccaggcg atgaacctgt tcgtgtcctc gctcgcatca gggccggggc gggcagagcg
    23701 caccaatcga aggatagctt cgacaacctt gtccggcacg gggatctgcc gcaccaggcg
    23761 ctgcatcgcc atcaactcct ggcccgtgag gatcggctcg agccgccgct cctcggtgcc
    23821 ggtcgtcgcg aacagcatcc ggcgttcggc aacgagatcg ggatagttga cgtcgatctg
    23881 caacagaaag cggtcgagct gtgcctcggg aaggggataa gtgccttcct gctccagagg
    23941 attctgggtt gcgagcacgt ggaagggttg gggcacgtca tagcgctggc cggcgacggt
    24001 gacgtggtgc tcctgcatgg cctgcagcag ggcagactgc gtctttgggc tggcgcggtt
    24061 gatttcgtca gccatcagca gctgagtgaa gatcggcccg gcaatgaaac ggaagctgcg
    24121 gcgttcgccg ggaatttcct ccagcacctc cgagccgatg atgtcggaag gcatcaggtc
    24181 gggcgtgaac tggatgcgct tggcttcgag gccgagcacc ttccccagtg tctcgaccag
    24241 cagcgtcttg gcgaggcccg gcacaccgat gagcagcccg tggccgccag cgaggatcgt
    24301 gatcagcgtc tgctcgatca ctagatcctg gccgaagatc accgaggcgg tggctttgtg
    24361 cacgcgggac atgcgctcgc cggccgcttc gatgcgggcg acgatgtcgt catcagtgtg
    24421 ggtgagcgtc gtcatggcct ctactataga ttgggtctgg tattgcacaa actctgctgc
    24481 ggcgaaattg gtcacaatat ggagcgggcc aaacgaatat gaagagttcc gagtaacttc
    24541 tgccgctcgc aagaaattga tcgagtactg cacgagggaa tgatgacgca gcaggcaagc
    24601 gagggcgcaa acgtgcgcat tcagggcctg gatgaactca tcaaggcggc tcaaggagac
    24661 cggcttccgc ctgtgcagaa gtggaacccg ccctactgcg gggacattgg aatgcggatc
    24721 cgggccgacg gcacatggct ttatcagaac agcccgatcg ggcgcaaacc ccttgtgaag
    24781 ctcttttccc gcatcttgcg gcgggacgcg gacggtcgca cctacctcgt gaccccggtc
    24841 gagaaggtcg acgtcacggt cgacgatgct ccgttcctgg ccgtcgagat gcaggtcgag
    24901 aaccgcggcc gcgatcagca gctcattttt cgcaccaacg tggatgacat cgtgcgctgc
    24961 gggccggagc atccgctgcg ttttgcctgc gagcccggca gcaatggact gaagccatac
    25021 ttgctcgtgc ggggacggct ggaggcgctg gtcacgcgcg ccctctatta cgatctggtc
    25081 gagctggctg agcctgggcc tggggccgaa ggcgggctcg gcatctggag cggcggcgcc
    25141 ttctttgcac tggaagcgga ggaagagaag gatgctggtt tttcgccttg atgggcgcta
    25201 gtgctggagc tcgtggtggt gcgcaaccat ggaggcaccg gctggtacca ttccatggcg
    25261 aaatcgatga atgcccgtac cttggccgag agatgcatcc ggtatggata gacgaggtgc
    25321 acgccgaacg ggtagtccgg cttgtcctcg aacagctcga cgagctcgcc gcgggcaatg
    25381 gcgcgcgcaa ccacgaattc cgggcagatg ccgatgccta agcctgcgat caaggcctgc
    25441 ctgacggcgg ccgcgctgtt cacgctgagc aagggggaga tctttatttc gatccgctgg
    25501 ccattatgca cgaagcgcca gttttctcct cccggcaaat tcgtatccag gatgcaggcg
    25561 tggtttgcga gctcctcgat tgaggctggc cagccgtacg catcgaggta ggccggcgag
    25621 gcacagatca cgaaaggaag ctcgcagagg cggcgactga tcaacgacga gtcgatctgc
    25681 tttgcaatgc gaaaaccgat ttcgaatccc tcgcggatga gatcgacgtg gcgatccgtc
    25741 agcagaacgt cgaattcgac ctgcgggtgc acacggcgaa acgcactcgt catttccacg
    25801 agctcgagtt cgccaaagaa ctggggcgcc gtgagtctca acagcccgcg tggattgccg
    25861 ccgccggtgc gcacggcttc cgtcagcgca tcgagctccg ccagagtttc ctgcacccgt
    25921 tcacagtagg ccttgccgat ttccgtgaca gcgaccctgc gcgtggtacg gttgagcaga
    25981 atggcgccga gccgccgctc gagctctgcg atgtgcttcg agacaagtgc cttggacttc
    26041 ccgagctgct gaccagccgc cgtgaagctc cccgcctcca cgacggcgag gaatgtgcgg
    26101 atgaggtcca ggctgtccaa ggctgctccg ctcccagacg atttggctca ttgagagcat
    26161 tatcgctcga gaggttggag tcaaattagc cgaaagccta aacttgagtc attttctgtt
    26221 attcgagcga ggctcgcggc gacattcggc gcccgtggcc tcagtgtagt cgaaattcgc
    26281 cttcgacgaa gcgcacctca ctcgggtgga tcagccgctt gtgcgcgatg cgaacccgat
    26341 agagcttccc atccagattt ttcgaccaga aagccagaaa cttggcgagt ttcggaaatt
    26401 caggatgcaa gtcgtagtcc tgccagatga agctctgcag gagcgaagga tgatccggca
    26461 tgcgataaag gatctctgcc gtggtgagac tgaagccttt cagttgcgcg gcgaagcttg
    26521 cgtcgaccat cgcagaatgt ccttatttgc gtctccacaa ctgctaataa cgtatagctg
    26581 tttgaccggg tggcaagggg gattttcacg aaaaaacaac gtgttagcag caattgcttt
    26641 ttgctgctaa cagcttcgcc gaatgcgcct cacccggcgc ggccgccgaa atcccgtaag
    26701 ccgagcgtca gtacagaact cgaattccga ccagtcgccc tgccgcaccg cgtggcctca
    26761 ctctcaccaa agttgcccat gtgttttctc acaaaaggat gagaaacagg tctccccgga
    26821 gttgacgtgc gatgatgaat cctgaagcgg tgcgcgagcg catcctcgca catgcggtca
    26881 cagcggccag tgcacatggg ctgaaatcgc tgtcgatcgg cgagatggca cgcgactttg
    26941 cgctcccgaa aagccgcctc ttcaccctct ttccgtcgaa cgaggccctc cagatcgccg
    27001 ctctcgagca cgccgccgca gcgttcgcga aagatgtggt cgaggcggcg ccggagagcc
    27061 ttccggcgaa ggaacgtgtc acgacacttt tcgagcgttg gctggattgg tcgcgttcgc
    27121 gccgcctcac ccgcggctgc cccttcgtcc acgccagcgc cgaaggcgag aacctcccag
    27181 aatccgtgaa cgccaagctg atggagattc tccagcattg gtcaggcgtt ctgcgcaaag
    27241 ccatagatga cgccaaggcg cagggcgtcg tgcgcggcga tgtggatacc gaccagttcg
    27301 tgttcgagct ctacgggctt tacctgtccc accacttctg gcattgggcg atgaaggatc
    27361 cggacgcccg ggcgcgcacc atgcgggcat tcgatcgcct gctgggcagc gccctttgcc
    27421 gctgagccga tgaggttggg cacttgctct gggggccact tgccttttgc ggcggtccgt
    27481 cgcagactgc gcgcgttccg aggggggccg gcggcagccg gctgagatgg cgctgaagcc
    27541 gcgccgccac cctttgaacc tgatccgggt catgccggcg aagggacgga cacttgcgca
    27601 aaccctcctt ctccgtcgca ttccgggatt tcgagcgatg agcgacttgc gcgcgtcgtc
    27661 aatggagcga cctgcgcccg atccgggagt gaagtgatga acaagatcac ccgccagagc
    27721 gagctgatgg tgccgaaggt caccaccggg ccgatcaacg ccagcacgaa ggtctacgat
    27781 tcccccgagg gccatccgga gctgcgcgtg cccttccgcg agatcgcgct caccgatccg
    27841 gaccagccga cgttccgtgt ctatgattcc tccggcccgt acaccgacgc gagcgcgacg
    27901 atcgacgtcg agaagggtct gccgcgcatt cgcgaggctt ggatccgcga gcgcggcaac
    27961 gtcgagcagt acgacgggcg cgaggttagg ccggaggata acggcaatgt caccggcaag
    28021 gcgctcgccc gcgacttccc gaataaggcg aggcccttcc gcggcgtcga gggcaagcct
    28081 ctgacgcagc tagagtacgc ccgcgccggc atcatcacca aggagatgat ctacgtcgcc
    28141 catcgtgaga acctgggacg caaggcggtg ctcgagcgcg ccaagtcggc gctggccgat
    28201 ggccagagtt tcggcgcctc cattccggag cacattaccc cggagttcgt acgcgacgag
    28261 atcgcccgtg gccgcgccat cattccggcg aacatcaatc acaccgagct cgagccgatg
    28321 atcatcggcc ggaacttcct ggtgaaaatc aacgccaaca tcggcaactc ggcggtcacg
    28381 tcctccgtcg aggaggaggt cgagaagatg gtgtgggcga tccgctgggg cgccgacacg
    28441 gtgatggacc tctccaccgg acgcaacatc cacaatacgc gcgagtggat cctgcgcaat
    28501 tctcccgtgc cgatcggcac cgtgccgatc tatcaggcgc tggagaagtg cggtggtgat
    28561 ccggtgaagc tcacctggga gctttatcgc gatacgctaa tcgagcagtg cgagcagggc
    28621 gtcgactact tcaccatcca cgcgggcgtg cgccttgctt atgtgccgct gacggccgac
    28681 cgcgttaccg gcatcgtcag caggggcggc tcgatcatgg ccaagtggtg cctcgcgcat
    28741 cacaaggaga gcttcctcta cgagcacttc gaggacattt gcgacatcat gcgcaagtat
    28801 gatgtctcat tctccttggg cgacggcctg cgtcccggct cgatcgcgga tgccaatgac
    28861 cgggcgcaat tcgccgagct ggagaccttg ggtgagctga cccagatcgc ctggcgcaag
    28921 ggctgccagg tcatgatcga gggccctggc cacgtcccga tgcacaaggt caagatcaac
    28981 gtggacaagc agctcgagac gtgcggcgag gcgccgttct acacgctcgg gccgctcgtg
    29041 accgacatcg cgccaggcta tgaccacatc acgagcgcga tcggcgcggc gatgatcggc
    29101 tggttcggca cggccatgct ctgctacgtg acgccgaagg agcacctggg gctgcccaac
    29161 cgcgacgacg tgaagacggg cgtcatcact tacaagatcg ccgcccatgc cgccgatctt
    29221 gccaaggggc atcccgcggc acagctccgc gacgatgcgc tgtcgaaggc gcgtttcgag
    29281 ttccgctggc aggatcaatt caacctgtcg ctcgatcctg atacggcgac cgcctaccac
    29341 gacgagacgc tgcccaagga ggcgcacaag gttgcccact tctgctcgat gtgcggcccg
    29401 aagttctgct ccatgaagat cacgcaggac gtgcgcgact acgccgccaa gcagaatgcg
    29461 ccgatcgacg tcgaagccgg catggccgaa atgagcgcca agttccgcca gatgggcggc
    29521 gaggtgtacg tggacgccga gaaggccaag gaggcgaaca gggcgttggg gtagcgccgg
    29581 cgccgcagcc tctttgcctc gcatcctgaa cgcactgccg tgcacaatca gtgcagcgcg
    29641 gcatggagcg atgatattgc gggtcggcgc tcaccaaggc agcgcgccga tccgttatcg
    29701 caagatgtct cgtcagccgg cgtccgtcgt ctcagccggc cggtcgtcct tcaggcgctg
    29761 ggccagcgcc gcctcgatga acggatcgag gtcgccgtcg aggacggctt gcgggttgcc
    29821 gctctccacg cctgtccgca cgtccttcac gagctgatag ggctgcagga catacgagcg
    29881 gatctggtgg ccccagccga tgtccgtctt ggccgcggcc tcggcattgg ccttttcctc
    29941 gcgcttcttc agctcgaggt cgtagaggcg ggcgcggagc atggcccagg caatggcccg
    30001 gttcttgatc tgcgagcgtt cctgctggct tgccaccacg atgccggtcg gcagatgggt
    30061 gatgcgcacc gccgagtcgg tcgtgttgac gtgctggccg ccggcgcccg aggcacgata
    30121 cgtgtcgatc cggcagtcgc tctccttgat atcgatgtcg atgttgtcgt cgatgaccgg
    30181 atagacccag accgaggcga agctcgtatg ccgccgcgcg ttggagtcga aaggcgaaat
    30241 gcgcaccagc cggtgcacgc cggactcggt tttcagccat ccgtaagcgt tctcgccctt
    30301 gatctcgaga gtggctgact tgagccccgc ctcctcgccc gggctcgctt ccaccatggt
    30361 caccttgaac ccgtggtcct cggcccagcg gatatacatg cgcgtcagca tctcggccca
    30421 atcctggctc tccgtgccgc cggcgcccgc atggacttcg aggtaggcat cgttgctgtc
    30481 cgcctcgccc gagagcagcg cctcgatctc acgcttctgg gcttgttcgc tgagcttctt
    30541 cagcgccgcc tcgccttcgg cgatcgtcgc ttcgtcgccc tcggcctcgc cgagctcgat
    30601 gagcgtgatg gcgtcgtcca gctcctgctc gagctgcttg taagacgaga tctggcgttc
    30661 cagggtctgc cgctcacgca tgatcttctg cgcggcgcgc gcgtcattcc atagcgtcgg
    30721 gtcttcggcc cgggcgttca gctcggctag gcgtgcttcg gctgtatccc agtcaaagat
    30781 gcctccgtag gagggccagt gactggcgaa tttggtcaac gtgcttctgt ggctctgccc
    30841 gcatggcagc ctcctcgggt cgtgatgcgt tgtgattgga tggccctgat ataggggcgt
    30901 tgcagcctcg tgtaaacgcg tcggggccgg cagaaagggc tggcgctccg gcccccaaag
    30961 ggttgagggc tacgacaccg gccaccaccc gtgagtgggg gtgactcagt tttgatcaac
    31021 gcaaaaaatc tagtagacgt tgccgaaacc ggagctcacg ttgcgtggtg gtggggaaac
    31081 gccatcgcca taggagctcg tctcatatcc caagaaggaa gcgggatcgt tcggctcctc
    31141 gccgggcttg aatacttccc agatgacgtc cttctcgccc ggcgaggccc tgaggccggt
    31201 gtagcggttg accggaactg tacgaatgcc gggcggtgcg cggaacggca ctttcttgtc
    31261 gtcgagcgcc atcttcatga aattggcgaa gatcggcgca gcaacctgac cgccggtcgc
    31321 cgacttgccc attggtttgg gcgtatcgta accgacgtag acgccgacga cgaggtcggg
    31381 ggagtaaccc acgaaccaag cgtccttgga ttcgttggtc gtgcccgtct tgccggcgag
    31441 cggagtgccg ggaagaacgc gcttcacatt cgttgccgtg ccgcgctgga tcacgccttc
    31501 gaggatcgag gtcatctggt aggcggtctg aggatcgaga acctgcttgc ggtcgtccgg
    31561 gatctccggc tcttcctggc cccgccatgc atcggcatcg cagtcggggc attcgcgctg
    31621 gtcgtggcgc cagatcgtgc gaccccaacg gtcctggatg cgatcgatca gcgtgggtct
    31681 caccttcttg ccgccgttgg cgaagacgga gtaggcggcc gccatcctga gcagggtcgt
    31741 ctcgccggct cccaatgaca tcgccagcac cggcagcaga tcatcgtacg cgccgaaccg
    31801 cttcgcatat tcgatgatga gcggcattcc gagctcctgg gccaggcgcg ccgtcatctg
    31861 gtttcgcgac ttctcgatgc cgatccgcaa tgtcgacgga ccgtaggaca tctccttgtt
    31921 gtagttctgg gggcgccaga cctcctgacc cggcccctgc tcgatctcga tcggagcgtc
    31981 gagcacgatg ctggtcggcg tgtagccgtt gtcgagggcg gcggcgtaga ccaacggctt
    32041 gaacgcggag ccgggctgtc gctttgcctg catcgcccgg tcgaactggc tgatcgagaa
    32101 ggaaaagccg cctacgaccg cgagcacgcg gcccgtgtgg gggtccatcg cgacaatgcc
    32161 gccgccgacc tctgggatct gcatgaggct ccaggcgccg gtcggctcgt tcggatcctt
    32221 cggcgcgacg tagatcacgt cgccaacggc caccacgtcc gccggcttct tgggcgccgg
    32281 gcccttgggc ttgtcggtcg cccggaatgg ccccgcccac ttcatttcct cgaacgcgat
    32341 ctcgaccgct tccctctgct ccgggatctt cccattcggc tgacggtccg gcctgagccc
    32401 gacgatgatc ttgtcccgct ccacctggag cacgacgccg agacgccacg gctggatgtc
    32461 ggaaggcacg tccattttgg cgagcgttgc gccccaatca gccgtcacgt cgatcttgtc
    32521 gacggggccg cgccagcccc gctggcgatc gaaggcgacg aggccgtcca tcaacgcctg
    32581 gcgcgcatag ccctgcagat cggggtcgag cgtcgtgcgc accgacaggc cgccaccata
    32641 gagcttgtcc tcgccgtatt tggcgagaag cgtgcggcgg acctcctcgg cgaagaactc
    32701 cgcggcgaag atgtgggcgc cgaacgggcg caggttgacc tgcaacggct tggccttggc
    32761 cgcctccatc tgctcgtgcg tgatgtagcc gttctcggcc atttgctcca acacccagtt
    32821 ccggcgggcc gtcgcctccc gagtgttgcg gaacgggtgg tagttgttcg gtgccttggg
    32881 cagcgccgcg aggtaagcgg cctcctcggg cgtgagctca tccagctcct tgttgaaata
    32941 gttgagcgcg gccgcagcga caccgtagga gccgattccg agataaatct cgtttaggta
    33001 caactcgagg atcttgtcct ttgagaatgc tcgctcgatc cggatcgcga ggatcgcctc
    33061 tttgagctta cgctcgtatt tctgctcgct ggagagcagg aagttcttgg ccacctgctg
    33121 cgtaatggtc gacgctccct cagggcggcg gccgggattc tgcaaatttc tgatgaatgc
    33181 acggacgatg ccgaagaagt cgattccgcc gtgctcatag aagcgcttgt cctcagccga
    33241 gaggaaagcg tggatgacga gcttcgggat ggtgttgatc gggacgaaga tccgccgctg
    33301 acgggcatac tcggcaatca gctgtccgtc gtgcgcgtga atgcgcgtca tcaccggcgg
    33361 ctcgtactgg gcgaggcttt cgtagctcgg cagatcgcgc gagacagacc acaggagata
    33421 gccggcggcg gccgatcccg cgacaaaggc caccacgccg gcggcaaaca cccagccgag
    33481 caggctcaac agaacgctct tccggcgttt cctcttccgc ttcggcgtag cgggcggtgg
    33541 cagaatcggt gctcgcatca ggccttctag ctttcaggat cgctcgcggt tcctcacaca
    33601 gccaagcggc aacaaaacgg catcggaaca gaacggtgtc ccgggtccga agtccgccag
    33661 agctcgtagc cagcgtctgg agcatgcttc ggcaaacaag gacgaggcac gagaaactcg
    33721 agagctgcca gcgtgattca ggtcggccct cacactatca cgtattgccg gatctgttcc
    33781 ggaaaatgcg ggctcaacgc acgatattct tcagcgcgac cggcgagcga cagtgaaagg
    33841 agcagtccct gcgctatcca ctgacaaatt acggcgcatg caccatgccc atagcgcgcc
    33901 gtgcgaagaa cgaatcgatt gctgcaagca tggcgccggc gactttgtcc tgccatgccg
    33961 gcgacgtcag aagctttcga tcctccggat tgcttacata gccgagctcg accagaacgg
    34021 agggcgtttc ggcctgcttc agaacctgga aagcggcaga gcgctgaggg tcgcgggcga
    34081 gcacgatgtg cttgcgcagc tcgccggtca gcaggttgct gaaatcggcg gagaagttct
    34141 ccgtctcccg ccggagcagg tcgatgagga tgtcgcggac ctcgtcctcc tcctggtccg
    34201 ccgtcgattc aagcccggca aggagatcgg cagcgttctc cttgtccgcc agtctgcggg
    34261 cctcctcgtt ggaggcgcga tcggaaagcg tgtagatgct gccgcccctg acgtggggcg
    34321 ccaggctttc ctccgcgacc gagtccgcgt gaatggagat gaaaagatcg gcgccgtggc
    34381 gccgggacgt gcggacccgt tgctccaggg aaatgtacat gtccctgttc cgcgtcatga
    34441 cgacggtgta gcggcctgac ctgacgagca gattgcgcag gcggcgcgcg acggaaagga
    34501 cgatatcttt ctcgggcagc ccgtcggggc cgatcgcacc gggatcgacg ccgccatggc
    34561 cggcatccac gacgaccacc ggcttgcctt tgcgcagggt cacggcgggc ggcggttctt
    34621 cgaatttgct cggccgcaat acgggagcag cgtcgacggc cggggtgtcc ggggcagggc
    34681 gaatcgcttc ccgggcgagc tcgatcacga ggcgcgcggt ctggccagat gtttcaaccc
    34741 gcgccttgcg cacgtggacc ggcccatccg tgtcgatgac gacgcgcgac tgcctggggc
    34801 cgaacaggcc ataccggaag gctttgatca ggccctttcc gttttgtccc accgtcgatg
    34861 cgattcgaaa atcaacttcc ggcaggtcca cgatgacgcg gtccggctcc gtcatggtcg
    34921 agacgctgat cgcaacctcg cgcgtcagct cgagctccag gcgcgtcagc ttgtcgttcg
    34981 ccgtgaggtg tgcccgcgtc acggccggcc ttcccgccgg cccttccgag ttgacctcga
    35041 tggtccatcc cgtcgcgcgt ccggcttggg cgagcacgca tgcggccccg accaaattca
    35101 atccgatcaa agccgccatg gtggcagcga gcactcgccc cccgtttcgc atgtgctccc
    35161 gcgcgcctcg cgtcgttcac atttgttgtc gcaaaattgc gccgctggtt aacgggttat
    35221 tggccaatgc agcccggcac aagggggctt tgagcagctc gcaggcgtgc gtgacatcca
    35281 caacactctt gtctcggcgg gggggagcac ttaaaacaca attgcaaccg tgccggggct
    35341 cgaactttgg gcgcgaccgg cggcggagga cgagggagcg agcagcgcat gccgcgccac
    35401 ggaagttccg ccaggctcag cgtacacggc ccaaccggcg cgtcatgggt gtgcagtcgg
    35461 cttcgagcgg cggcttcctt tcccttggca ccatcacgtc cgggaatgga gcgagcgcgc
    35521 ggtcttgcag gcgaccaccc gagacttttc gccctgcttc tgctgcccgg gaaaggggtg
    35581 cggctggcag ggcataagcg gcgactttgc aaagaggcgc cactcatctt gcccggcgac
    35641 gggctcagct cttcatcgag cttcagaaat gagcttgcgc gaagagcggg ttcaccaagc
    35701 aaccgatgga aaacacgttc acaaaatcgc agtgcagtca ggagctttgc gtttccccac
    35761 tccggtcggg gttggtcctg ctgcggcatt gctcgagcgt gacgcacgcg ccgcagtcgc
    35821 agcgggcgtg ccctggaccc ctttccagat tttatgattc ccagcaactg aacacgcgcg
    35881 ccccaggccg gcgaacccct cgccgccatt cggcagccac gatcggtgtc acgcccggcc
    35941 ggcgcgccaa ggaacaaaac acatgtcaag caacaagatg ctcatcgatg ccacgcatcc
    36001 tgaagaaacc agggtcgtgg ttctccgagg aggccgtgta gaggaattcg acttcgagtc
    36061 cgcggctcgc aagcttctcc gcggcaacat ttttctcgcc aagatcaccc gcgtcgaacc
    36121 gtcgctgcaa gcggcgttcg tggactacgg cggaaatcgc cacggcttcc tcgccttcaa
    36181 cgagatccat cccgactact accagatccc cgtcgcggac cgtcaggcac tgatcgatga
    36241 ggaagcggcc gccgaggccg aggacgaggc tgccgccgac aggcaggccg aggaacgatc
    36301 acagacccat ggagatcggg aggaagacca ccccgacggc gaggggccgg aggaggtcgg
    36361 cgtagacgtt acggcgttcc tgccccctga gcccgaggca ccttccgagc cacaggaagc
    36421 cgctccatcg gccgaaaccg cagggcctcc cgaagaagag gccgggtcgc gcacgattac
    36481 ggaggctgtg cagcccgagg agcgtctgac cgaggccgta ccgagtggaa cggccgagaa
    36541 cggccacgtc gccgagcatt cggaatcccg cgaggacgat tccggcgcgc gtgcggacgc
    36601 atccggcgcg cacgagcctc ggtcggatga gaacggctct gatgcctccg aagaggcttt
    36661 gagcgatgcc ccgaagccga tcggtctcgt ggaggaggtc ggggtcgagg aagtcgagga
    36721 gaatgggaac ggcaacggcg gcgtcgagca ggtcgataac gtcgtcgcag aagacgcgct
    36781 cgaagagctt ccgtcgcgcc cgcgccgccg ccagcgcagc tacaagatcc aggaggtgat
    36841 caagcgccgg cagatcgtgc tggtgcaggt cgtcaaggag gagcgcggca gcaagggcgc
    36901 ggctctcacc acctacctct ccctcgccgg ccgctacacc gtcctgatgc cgaacaccgc
    36961 ccgcggcggc ggcattagcc gaaagatcac caacgttcag gaccgtaagc gcctcaaggc
    37021 catcgcggcc gagctcgacg tgccggaagg aatgggtctc atcatccgca ccgctggcgc
    37081 gtcacggacc aagcaggaga tcaagcgtga tttcgagtat ctgctgcggc tgtgggaaag
    37141 cgtgcgcgag ctgacactgc gctcaaccgc cccggcgctc gtctacgagg aaggcgacct
    37201 catcaagcgc tcgatccgcg acctctatac caaggacatc gacgagatca tcgtcgcggg
    37261 cgaggcggcc tacaaggaag cccgtgactt catgcgtatg ctgatgccaa gccacgtcaa
    37321 gaacgtggtg ctgtatcagg accccgagcc gctgttcagc cgctaccaga tcgagcggca
    37381 gctcaatgcg atgttcagcc cctacgtcca cctgaaatcc gggggctatc tggtcatcaa
    37441 ccagacggag gcgctggtcg ccatcgacgt caactcgggc aaggcgacgc gcgagttctc
    37501 catcgaggaa acggctctca atacgaacct cgaggcggcc gacgagatcg cccggcagct
    37561 taagctgcgc gacctggcgg gcctgatcgt gatcgacttc atcgacatgg aggagaagcg
    37621 caacaaccgg gcggtggagc gccgtctcaa ggaggcgctg cgcttcgacc gggcgcgaat
    37681 ccaggtcggc cggatcagcc acttcggatt gcttgagatg tcgcggcagc ggctcaggac
    37741 gggcgtgctc gaaggctcga cctcgcagtg cccgcactgc cagggcacgg gcatcatccg
    37801 ctcgaccgag tcggttgcgc ttgccgtgct gcgcgccatg gaggacgcgc tgatgtccgg
    37861 cccgccggca cccatcgtcg ccaagacgac ggcgagcgtc gcgctctaca tcctcaacaa
    37921 caagcgctcg ttcatcaacg agatggagtc ccgctacaag gtccccatct acgtgcaggc
    37981 gagcgacaag gcgcagggcg cgaacttctt catcgagaag gccgcgcgtt ctgccgacgc
    38041 tcagcgccag ccggagcgca gcgccgtcag catggactgg ggattcgagg gcgaggagga
    38101 gccagagccc gcggctcgcg aggaggaggt ttcgcatcac gaggccgagg acggcgaggg
    38161 ggcccgcagg tcccgccggc gccgccgtcg gaggggccgt cgcagtgaac gcggcgagtc
    38221 tttgggggtg acagcagtcc cgggcaacgg cgcggacgag gaaatcgcat attcgtccga
    38281 gggtgcggac actgaagatg acgtcggcgc ggccgaagcc gccgaagacg aggctggtgc
    38341 tgaggttggt gccgcggcgg tagaagagga gggcgaggct cacgaggatc gccggaaccg
    38401 ccgtggtcgc cgccgtggcc gccggggcgg ccgccgcgat cgtggtgaag gctcccgccc
    38461 tggagcggag gcagcagaag ctgccggcga ggtcgaaccg gacgaggcag agagggaggc
    38521 cgcagaaccc gagctcggcc tgcccgtagc ggagccggtt gcggaacggg ccccggccgc
    38581 agcggttgcc gcgcagcccc ttgcgacgga gtccgccgag acggtcgccg aagcggcgcc
    38641 gcagccgccg gcggctgctg ccgaggccgc ctctgaggag cctgcgccca cgagtccggt
    38701 ggaggagccg gaggctcagc ccttggctgc tgcagctgag ccggcgccag ccgaggatca
    38761 gatccccgag ctccaggccg gcgaggccag gccgggacga aacgcccgca ccgaaccggt
    38821 cgccagcgag ccggtgatcg agcggatcgt ggtcggaccg gaaatcggga ccgcggaaca
    38881 gccggcgcct gcagagccgg tgcgccgcgg ctggtggcag cgccgcttcg gcgccaccac
    38941 ggaatgagga ccgttcactc gaaccgcctg ccagttctaa tgacgaaacg ggggagcgcc
    39001 gatcagcgct cccccgtttc atccttgaat tgctcttgtc cgcggagcag attcagcctt
    39061 tgctccgcat cgccgccatg aggctcgagt cgtagtagtg ctccgcgatc tgccagaagt
    39121 agaggacctc gttgcggaat gcgtggtgtg actcgagcgc ccgtttgaac agctcgttct
    39181 cggccgcgat ctcggcgtag agctcatttg cagccttcca gcaggcttcc atcaccggct
    39241 gcgggaacgc acgcagctca gcaccggcgg caatgagccg cttcaaagcc ggagcgttca
    39301 cgaggtcgta tttcgcgatc atccagttgt tggcggcttc ggcggcgttg ttgacgatcg
    39361 cctgatacgt cttcggcagc gattgccatt tctccatgtt gacgatcagg tgcatcatgg
    39421 caccgccctc ccaccaaccg gggtagtagt agtatttggc cacccggtgg aaggcgagct
    39481 tctcgtcatc atagggtccg acgaactcgg tggcatcgat ggtaccgcgc tcgagcgcag
    39541 ggtagacatc gccaggcgcg atctgctgcg gaaccacgcc gagcctcgcg agcacatgtc
    39601 cgcccatgcc gccgatgcgg aacttcaggc ccttgaggtc ctcgaccgtg ttgatctcct
    39661 tacggaacca gccgcccatc tgggtgccgg agttgcccat cacgatgccg tagacattga
    39721 acttgccgag cgcctcgttg atgatctccc taccgccacc gaagtgccac caggagtgct
    39781 ggtgacgcgc attgaggccg aagggcaggc cggtgccgaa agcaagcgtc ggctccttgc
    39841 cgatgtagaa atagaccggc gtctgagcac attcgacggt cccgacggag accgcatcga
    39901 gcgcctgtag cccggggatg atctcaccag ccggaaacgt ctggatctgg aacttgttgt
    39961 cggtcgcctc cgccacgtac ttggcgaacg tctgtgcagt gccgtaaatg gtgtcgagcg
    40021 accgtgggaa gctcgaagtc aggcgccagc ggatttcagg cgcactctgc gcgatcgctg
    40081 gactcgcgag cgccgatgct gcggccgata ctgcaccggc cttgaggaag gttctgcgtt
    40141 tcatactggt acgtgcccca ctagagacgg tcagaagtcc gatgcctctg attgcagtgg
    40201 cggactcgcg cccgcaggcg cacagagaaa gacgcgccag cagccgttct tgatcagcca
    40261 cacgtctttg atgcgcggat tggctggcag aggtcaaggt gcatgtgccg cgagaaccga
    40321 cgagctggtc aagaaatgga cagctggtgg cccctcagcc tttccgccgg agaacgatgt
    40381 cgagcacgcg ctccggcgtc ggctcccgtt cgaggcggaa gaagtcgcca tcggtttgga
    40441 gagctcgttc gagctgccga tgaaactcgg atcggtccac ctcgatggtc ccgaactgcc
    40501 tgaggtgatc ggtcacgaac tgtgtgtcga gcatgacgaa gccgccgaaa atcaggcggg
    40561 cggccagata gacgagggcg attttcgacg catcgcgcat ctctgagaac atgctctcgc
    40621 cgaagaaggc gccgccgagc gccacgccgt agaggccgcc caccagctgg ccctggtgcc
    40681 aggcctccac cgtatggcaa tggcccaacc ggtgcagctc gcgatagagt ttgcggatac
    40741 ggtcgttgat ccaagtcgtc cggcggccgg ggcgcgaggc ggcgcaaccg gcgatgaccc
    40801 cgtcgaagtc cgtgtcgatg cggatctcgt acggcgcctg ccggatggtg cgctgcagtc
    40861 gcttcggtac caagactcgg tcgagcggaa ggacgccgcg ggcctgcggc tcgatccagt
    40921 aaagctgcgg atcatcggcg ctttcggcca tggggaagat gccgcacgtg taggccttca
    40981 gcaggacctg cggagtgatc tcgagcagcg ggctttcgcg agcagtcatc tgcctttcac
    41041 gcctgttaca gaggcttggc accggaatgg cgtcgaacgg taatcggcga aattatccgc
    41101 ggttccaacc gccctgggtc tcgctgaaaa tattgcggaa tatcttaagc gtgagcaatg
    41161 ccaatccacc ttccctgttc gcgggcagga tcctggcctg gtgcccgaag atcaagccgc
    41221 tctccccccg gggcgatctc actcggcatt gccgaggtac ttctcgagcc agtggatgtc
    41281 gtagagcccg ttggcgatgt ccgcctgctt caccaagtcg acgaacaagg gaatggtcgt
    41341 ctcgatgccg tcgatgacga actcggacag gcaacgtttc aggcgcatca ggcactcgtt
    41401 gcggttcttg ccgtgcacga tgagcttgcc gatgaggctg tcgtagtacg gcgggatccg
    41461 atagccctga tagacgccgc tgtcgacgcg cacgccaagt ccgccgggcg ggtgccagta
    41521 ggtgatcttg ccgggtgacg ggcggaaggt gcgcgggttc tcggcgttga tgcggcattc
    41581 gatggcgtgg cccgaaaagc tcacctcctc ctggcgaagg ctgagcggca agccggcggc
    41641 gatgcggatc tgctcgatga ccagatcgac gccggtgatc atttcggtga cggggtgctc
    41701 gacctgcagg cgcgtgttca tttcgatgaa atagaactcg ccgtcctggt agaggaattc
    41761 gaccgtccca gcgccgcggt acttcagctt tcgcatggcg gcggcgactg tctcgccgat
    41821 cttctggcgg gcctcggcgt tgagagccgg ggaaggggcc tcttcccaga tcttctgatg
    41881 ccgccgctgg agcgagcagt cgcgctcgcc aagatgaatg gcgttgccct tgccgtcgcc
    41941 gaagacctga atttcgatgt ggcgcggctt gctcaggtac ttctcgaggt agacgctgtc
    42001 gtcgccgaag gccgccttgg cttcagagcg ggccgttgcg agcgcgacct cgagatcgtc
    42061 tggagagcgc gccaccttca tgccgcggcc gccaccgcct gccgctgcct tgatgaggac
    42121 gggaaagccc atatccctgg cgatgcgctg tgcgtcggcc tcgctcgtca cggcaccgtc
    42181 cgacccgggg acgacgggaa tgccgaggcg cttggcggtc tccttcgcct cgatcttgtc
    42241 ccccatgatg cggatatgct ccgacgtcgg cccgatgaag gtgatgccgt gctcttccag
    42301 aatttcggcg aaccgcgcat tctccgccag gaaaccgtag ccagggtgaa cggcgtctgc
    42361 gcccgtaatc tcgcaggcgg ccaacaggcg cggaatgttc aagtagctct ccgcggcagg
    42421 cggcggtccg atgcaaacgc tttcgtcggc gagccgcacg tgcatggcat cggcatcggc
    42481 cgtcgaatgc actgcgacgg tggcgatgcc gagttccttg caggcccgct ggatgcgcag
    42541 cgcaatctca ccacggttcg cgatcaggac tttgtcgaac atgtgtttcg agcgagcagg
    42601 aaagtagctg agccccaccc gccatgcgct cctattcgat gacgacgagc ggctcgccga
    42661 actccacggg ctggccgttc tcgacaagaa tatgggtcac ggttcccgac cgcggggccg
    42721 gaatgtgatt catggtcttc atcgcctcta tgatcatcaa ggtctgcccc tgcaccacgc
    42781 gcgagccgat ttcgacgaac ggggcggcgc cgggctccgg cgcccggtag gcagtgccga
    42841 ccatcggcga tttcacgcag ccggggtgat tggcgatgtc ggaggcaggc tcggcaggtc
    42901 cggcttggcc cgctggagaa acggggctcg ccggagcagc ttgctgaggc gcgacagcgg
    42961 aaaccgacag attgcgggca acccggatct tgagccccga cttctcgatt tcgatttcgc
    43021 tcaaacccgt ctcgacgagc agcgccgcga gatcgcggat catctgctgc tcggaattgc
    43081 cgccccgatc tcgttggtct ttcgccatgc ttgttggttc cgccatcgtt ccagtcgtgc
    43141 cggcccggct ccactcctca gggtcgagcc tgcccctgct gctccagaat gtccgccatt
    43201 gcctccagtg cgagctcgta cgtcttcggg ccgaagccgc agatgacacc ccgtgcgact
    43261 ggcgagatat aagagtggtg gcggaagctc tcccgccgga acacgttcgt caggtgcacc
    43321 tcgatcgcgg gcagctccgc cgcactcagc gcatcaagca acgctacgga cgtatgcgtg
    43381 taggcgcctg cgttcagaat gatgccgctg gcggcgttgc gcgcctgctg gatccaggtg
    43441 acgagctcac cttcgatatt cgactgccgg aagtcgatct gcaacccgag ctgtgctgcc
    43501 cgcgcctcga cccgccgctg tacgtcgccc aaggtctcgg cgccgtagat cgagggctcg
    43561 cgcaggccga gcatattcag gttgggtcca ttgaggacgt aaacaggctt tgccattgat
    43621 cagtctcgag gtgtggtcgc ggggcgtcgg gcatgtcgcc gggccgcggc tcatgcggct
    43681 gcggcccgtt tatcagcttt ccggcagtgg cggaagcgca ccgcgaacaa tgtcgttcgc
    43741 atcgtcgtgc cgatcaacag acttgacacc cctccttgcg cgcctcgccg atcatctggc
    43801 gcaggccgtc cagcgccagg aagccgacgt tcactttggc atccaccacg tagcccgggg
    43861 tgccctcgat agccaaggcc tgcgcgatcg ccgatgtctg cttgatggct gcctcgaact
    43921 ccggattgtc catgtcggcc ttcagcttct ccacgtcgag cccgactttt tgggcaatct
    43981 tgaacacgtt gtctttcgtg acctgccgct ccttcatgag cgcgatgtga aagtccaggt
    44041 acttgccctg ggctcgcgac gccatcgccg ccttggccgc gaagatcgag ttctcaccga
    44101 aaatcgggaa ttccttcagc accactttca cgttggaatc ggcttcgatc agcttcatca
    44161 gctcgccgac ggcgcgcttg caccaggggc agttgtagtc gaagaactcc acgatagtga
    44221 tgtcgccgtt gggattgccc aggacgaaat ccgccggcga agtgaagatc ttgtccttgt
    44281 tctcgaggat gatgcgcctg tgctcggcga cctgcatttc ggcctggcgc ttctcgagct
    44341 cccgtccaac ctcgaggagc acctccggat ttttgaccag gtaatccctg acgatgcgct
    44401 cgatggcttg tttctgctcg gccgagaatg ggcctgaggt atcggcctgg gccaccttga
    44461 tgttcgcccc ggtcgagacg gcgggagcac tgcccaacgc caacgcggag ccgagcgcca
    44521 gcagtgcaat ggcaccggcc accgcgcccc tccgtgtcgt cagggcatca atgattttga
    44581 taaggctttt ggcaggcatc cggaagtgtt ccttggttcg tgagtactat tcgataatgc
    44641 cactttagca cagcacagcc gtcgacccag tggtcgagga gcgagatgat tgagaaattc
    44701 ttgtgacaat tttgtcagcg gatttctcga tcgggggact acaggttgct ggcatctttg
    44761 tagcgtatga tgtcatccat tctgagccag gcaggggatc ccttcttcaa agcgcgctgg
    44821 gcacgctggg cgaaggtctg cgcctgcttg atgttgcctt caaggaaata ggcctgcgca
    44881 attgacgcat ccgcatcggc ttgccgcccc aggcgatagt acgcatcggc tagcgaccgg
    44941 taggcgcgcg ggttatcgtc ctcgacaacg gccttgcgca gcaggtcggc cgcctcgccg
    45001 accaccttcg ggtcgttcga ggcgagcaat gcggaggcga gttggacgcg gatcagcggc
    45061 gctcccttgg acaggcggag tgcctgccgc agcggtgcga tggcttcccg gttcttgccg
    45121 gagcgggtca ggaagtcgcc cttgagttcg tagaagtagg ggttgcccgg cacttcgcgg
    45181 atgagggcgt ccacctgggc gagagcggcc ggcagcgcgc tctggccgcc ctgcatgaag
    45241 ctggcaatcg cgcgggcata gcgggcgggc agggatgtat ccgaacgcgg atagcggttg
    45301 aagaccactt gcgggcgctc gagataaccc gccagcttcg ctcgcatcat gtcatggcgg
    45361 agctgcagtt cactcgaatc ccggttgtcg tagtacgggc tccgctgaac cagctcgcga
    45421 agacgcgcca gccggtccgc tgcaacgggg tgactgcgca cgaacgggtc cttctgcgcg
    45481 tcggagatgt actcttgctg ggcgaagcgc tcgaaggtct ccagcatgcc gcggcccgat
    45541 tggcgcgtcg cgttgagata gcgcaggcca gcctggtcgg cggccgcctc ctgcgagcgc
    45601 cgctcggcga gcagggagcg catgatgagc tcgtcgccag caaacaccgc ctcgccggct
    45661 cctgtggcga tggcaacgcc aagcccgagc atgcgcatga gcagggcgcg ggtctgatcg
    45721 cgtgcaatcc tagcgcgcag cgcagccatg tgcccgccgg cgatgtggcc ggcctcgtgg
    45781 gcgatgactc cgataatctc gttcggcgtc tccgactgca tcagtgcgcc ggtgtggacg
    45841 aagacgttac gcccgtcgag gacgaaggcg ttgaacatcc cgctgcgcac gatgcgcacc
    45901 gctacgcgat tttccccgag ccctgccgcc ttgaagatcg ggcgggcgta gtcgttgagc
    45961 aggttttcga tctcggtgtc ccggatgagt ggcagcccct gggccaacgc cggtttgctg
    46021 gtgacgctcg taaaggccag gaccgcaacc agggccgtcg cgaccagaca ggcgctcaaa
    46081 ggcgtctgtc ggccggtctt tcggttctgc agcatgtctc cagaagctct cgctcgttcc
    46141 tcgatggcgc ttcggttgtt gttccgtccc cctcacatct gggtggaggg agcctagcaa
    46201 ctaagcccag caaatcaacg ccgggctggt agagcatccg gctcgcctct ataagcggcg
    46261 tcggcgacgt gcgcccaacg ggttgttgga gcgagccaat ccagttttgg tttcatcgtg
    46321 gcattgcggc ggcaccatgg cggcaggcag acggaaagtg gacctcgacg gaggagcaga
    46381 actgatgacg ggacgttgcg atcagacccg cgaggacggc gtaccgcgac cgccctccaa
    46441 gcgcagtgcg atcgcatcgt tcatcgtcat ggacgtgatg cgggccgctg ccgcccgcga
    46501 ggctgcgggc catcgcgtca tccacatgga ggtgggccag cccggcacgc cggcgccagc
    46561 cacggcccgc gcggcggtcg agcgcgccat ggcaaccgac aacctgggct acaccctggc
    46621 gctcggaatg ccgagcctgc gcgagcggat cgccaaacac tacagggact ggtacggcgt
    46681 cgagctgtcg ccggagcgcg tcgtggtgac gaacggctcg tcggcggctt tcgtgctgac
    46741 gttccttgct ctgttcgatg caggcgagcg ggtggcgctg ccgtcgccgg gatacccgtg
    46801 ctatcgccac atcctcaccg cactgggcca gcagcctgtc atcatcgagg cagacgagag
    46861 ctcgcgctgg atgccgacgc cggatcagat cgaagctgcg agcgcccgcg accacatcgc
    46921 cggtgtgctg atcgcgagcc ctgccaaccc gaccgggacg atgctcgagc cgcaacggct
    46981 gaaggacatt cttaaggtct gccgccgccg cggactgtgg ttcatctcgg atgagattta
    47041 tcacgggctc acctacggta tgccggccga aacggcgctg aagcattccg acggcgtggt
    47101 cgtcatcaac agtttctcga aatatttctc catgacgggc tggcgcgtcg gctggatggt
    47161 cgtgccggag gagctcgtgc gtccggtcga gcggctggcg caaaacctct acatcgctcc
    47221 gccgacggtg tcccaggtag cggcgctggc agccttcgac ggcatcgagg agctcgaggc
    47281 caacaggcgt gtctacgcgg ccaatcgcga cttgctgctg agggagcttg cgcgagccgg
    47341 gttcgaggaa atcgtgcctg ccgatggcgc cttctatctc tacgcagacg tcagccgctt
    47401 caccgacgac agcctcgcgt tcgccaagct gatgctggag gagaccggcg tcgcgacgac
    47461 gcccggggtc gacttcgacg aagcgcgcgg ccggcacttc atccgctttt cttacgccgg
    47521 cacgacggcg gacatggccg aggctgccga gcgcctgaag ggctggacac ggctcaagtg
    47581 agctgccggt tcaaggcaga accgtgccgg gctgccggtc aggtggaaga tggcttgggg
    47641 cattctgcag gttattggcg gcttcgggac gcttgttgtg cgagcgctta ggtgtccgcg
    47701 gccgctccgc ggaaacatcc cggaaccaga ccgccaggaa atcgaggagc accctgagct
    47761 tcggcaggac gtgcttggag ctcggataaa ccgcccagac ggcggagttg ctggccagct
    47821 caaactcctt cagtacgcgc acgagccggc ccgcggccaa atcctctcgc acacgcagct
    47881 ccgaggtctg aaagaggccg tgcccatcga gagcggcatg gtgcaggatc tcgccgttgt
    47941 tcgaccgcaa ccgtccattc acccggatcg tgaagtcttc tttgcctcgg cagaaggacc
    48001 attgccagtt gtcgccgagc acgaaacaat tgtgagcgct gaggtcggcc ggcgtttgcg
    48061 gagtgccatt ggcggcgagg tagcttggag ccgcgacgac gacctggcgg tcggggcata
    48121 ggcgctttgc aatcaggacg gagtccgtca gcgccccggt ccgaatggcg acgtcgtagc
    48181 cctcctcctg cagattgacg ttccgatccg tcaggttcat ctgaatttcg atatccggat
    48241 aagcgcgcat gaacgagctg atcgccggtg ccacgaagag gcgcccgagc gtggccggag
    48301 cggaaatctt cagcttgccc cggggcttcg ttgcgtgatc tcccaccgca gcacgcgcgc
    48361 tttccagctc cgcgaggaca cgctcgacat gggtgagaaa cgtcgcgcct tcctccgtga
    48421 tacggatcga gcgcgtcgtc cgatcgaaga gcctgacacg cagctcgctt tcgagcgcct
    48481 ggatgcgttt ggagatcgtt cccggcgtca agccgagctc ggtgccgacg gacgacagat
    48541 tttgcactgc ggcgacgcgc gcgaaaatcc gcagatcctg aatatccatg ctcaaccctc
    48601 agccaccgac ccgcaaactc agtttccagc gtcggaatga ctccccctgc tgaacaatgg
    48661 taagcccggt gatgtaagcg ctaacatcat caggccgcaa gccttgcgtc tagcagaatg
    48721 cgcacgtcgc aataaaaaaa tcgcccggcc ccaccaagct gttgtccgct cgcctcgccg
    48781 cccgagcgca gtcttgcacg gatatttcgt cagaaacgac gcagcagacg ctcgatatag
    48841 tcgagctcga gcgccgggcg ggtggtctcg ccgaggcgcc ggcggagctc ctccaggatc
    48901 tccctggccc gctgaacgtc gatctcgtcg gggactttca ccgaggtgcc gaggtccggc
    48961 ccctccgtgc gctgcggacg tccgagcgga tcgcgcgggg catcgccggc agggccctgg
    49021 ccgaagcgcg aaggcatctg ccgcagcatc tgctcggcca tggattgcgc accttggcgg
    49081 agctgctcca gcgcctggga ctgctcgcgc gttgcggtgt caaggtcgcc ctgctcgagg
    49141 gcccggcggg cattctccat ggactgctct gcgccgtcga gctggcttgg cgggcgcatg
    49201 ccaaattgct gcaagccctc acggagctgg ccgaggcgat ctctgagctg gccttgccgc
    49261 tcggtgagct cgccctgccc gaaccgctgg ccttgctggc cccggccctg ctgaccgcgc
    49321 tggccctgac cgcgctggcc gaattgctga ccgcgttggc cgcgctgccc gcgctggccc
    49381 tcctgcccct ggcgctgctg ctcgccgaag gtatcgtcgt agagctgttg ctgcttgccg
    49441 atgatgtccc ccatctggtc catcatctgc atcatctgct gcccttgctg accctgctgg
    49501 cccatcatgc ggccagcctg cagcgactcc atcaactcgc ggagctggtc gagcatctgc
    49561 tgcgccattt cccgcgagcc ctggcgcgcc atgttctcca gctgatccag catgcgctgg
    49621 agatcctgcg gccgcatgat ctggttcggg ttgaagcctt cgggcaactg catctgctgg
    49681 ccctgggctt ggcgggccag ctgctgcagg aactcggcca tcgcttgccg cagctcctgc
    49741 atcagccgcg cgatctcctc gtcggaggcg ccctcctcga gtgccttgga aagctgatcc
    49801 tggatgtccc tgagccgccg ctcggcatcg gtcagctcgc caccatcctc gatccgcaac
    49861 gcgacgtgcc agagttgatc cacagagctg cgcaatcctt cgcgcgagct gtcctgcaaa
    49921 agccggtgat aaacggagcg caggccaaga tagacgttgt ggtcctcgat gaagccctcg
    49981 ggctcgaacg tcagggcatc gagcgccttc gaaaccagct cacgatagcg tggatcctcg
    50041 acgagcttga gccgctgctc gatcacggcg cgggcgagcg gcttcgtgaa ccggcgctgt
    50101 ggcagcacga tttcgatcgg ctggcttttt ccgacattgc cggcgtgatc cttggcgacc
    50161 agagtcatga tcactttcat gccggcgtac ggatgtgcgc cgagctgatg gaagctcgag
    50221 gtctccgagt cttttgcgtt ctgccgcggc aagcgcagat tcagcactgg cgggcgctcg
    50281 agaggcgggc gaggcccctg cagaatgtcc tcacgcgccc agctcgtacg cggatcgcct
    50341 gcaaccgtac gcggccgcgc gaaatgcgcc tcggccgagg ccacaccgta gtcgtcctcg
    50401 accttgtaaa agagcttcat gccgccacgg atcgtacgct cgggctcctt ggtcagaacg
    50461 atgcgcggag cctggtcggg cgtgacctgg aacctccaga cgacaacctc gctaccgccc
    50521 ccgagcacgc gtacggtgcc cgatttgcgc agctcggcgc gcacctccga gatgtcgccg
    50581 gcctgcttcg gctgccgtcc ctcgatgatc tgcggcttct cgctgttttg ctcgcccaac
    50641 tcgagtgaga gctggccaac accgcttgca cgaatgatca gcaggctctt gtcgggcacc
    50701 tcgatcggtc cggcttcagc atgtgaaggg cgcgagggtt cgctgccgcc gtcggccagc
    50761 aggaccggcg gcttgccggt gtaggctggc ggcgtcaccc acgcatcgag ccgcgcgttg
    50821 gcgagctgca ggtcagggcc gaaccggaag gcggcaaaca gccggtcctt cgcgctgtca
    50881 ccgacgaggc ccagcagcag cacgacgccg agcaccaata gagcgcggag cgcaaatgga
    50941 tccttgcgat ccgtgcgcgg ttgcggcggc ccgacgcgaa ggcgctcgag cagggcggcg
    51001 agcctggcct tgtgcgcctg ccagatgcgt tcggtggccg agccgctggc cgaagcggac
    51061 agcgtatcct cgtaggacga ggcgggacgg tgcggtatgc cggatcggcg ctcgatccgg
    51121 cggatgccct cctctcgtgt cggccagcgc acgcgcgcgg cgaagatgag ggcggcgagg
    51181 agcgcgaggc cgaaggcgct cagcacgatc tcgtgtgcga ccgggggcag ccagagccac
    51241 aggccggcaa acgagaccag cacgaacagg ccaagcacgc cgatgacgag ccaaagccgt
    51301 ggccacagct gctcgaagag cagggccagt cggctcaggc ggaccttgcg gttgaaggca
    51361 cgcccttcgg cggagggtgt ctgctggtct ttggacatgc tcgacaaact agcacacatc
    51421 ctctgcgtgc atcaggaaat tacacgaccg tccgctccta acacccggac cgccgcaaca
    51481 gttgcatcgt gcggggcaaa aagcggctgc acgagggcag gcaggttcaa gcgtcggtga
    51541 gccagtccgg cagccggtcg agatcgatca ggtcgtcata ggtcgggcgc gggcgtacga
    51601 cggcgtagtc gtctccgttg accagcacct cgggcaccag gaggcgcgaa ttgtaggtcg
    51661 aggacatcac cgcgccgtag gcgcctgcgg tcatgacggc gatgaggtcg cccgtctcga
    51721 gcggcggaag ttcccggccc agcgcgagat aatcgcccgt ctcgcacacc ggaccgacga
    51781 cgtcctgccg ctggcgttcg agttgcaact tggcctcccg caccggccag atgtcgtggt
    51841 aggcctcata aagagtcggc cggatgaggt cgttcatggc ggcatcgacg atggtgaacg
    51901 tcttggccgc cccgcgcttg gagtagatca cccgggacac gagcacgcca gcgttgcccg
    51961 caatcatgcg gccgggctcc agaatgaact tcagcccgag gtcgcccagc gcggccctca
    52021 ccgtcgcggc atattcgtcg gggtgaggcg gcacgtcatc gctgccgcgg tagggcacgc
    52081 cgagcccgcc gccgagatcg aggtgctcga gctcgtgccc ttcccggcgc aaggcaagga
    52141 ccagttcccg catcagccgg aaggcgtcac ggaacggctt gaggtcggtg atctggctgc
    52201 cgatgtgcat gtggatgccg gcgatcctga tcccagggag ccgggcggcc tgcgcgtaga
    52261 gcttcggggc ctcgagaaac gggatgccga acttgttctc cgacttgccg gtcgagatct
    52321 tcgcatgcgt acgggcgtcg acgtcggggt tgacgcggat ggcgacgcgc gccgtcttgc
    52381 gctgctggga ggcgacctcg ctcagcagct cgagctccgg ctcgctctcg acgttgaagc
    52441 cgagcacgcc ctcgctcacc gcataggtca actcgtcgcg cgtctttccg acacccgcga
    52501 agatgatctt gtcggcaggc acgccggcgg cgcgcgcccg gcgcagttcg ccctcggaga
    52561 cgacgtccat gccggcgccc agccgggcca tggtgcgcag gacggcctga ttggagttcg
    52621 ccttcacggc gaagcagatg aggtgatcgg tgccccggaa cgcctccgac agcacgcgat
    52681 agtgccgctc cagggtggcg gtcgaatagc aatagaacgg cgttccgacc tcgtcggcga
    52741 tccgtgccag gctgaccccc tcggcgtgca ataccccggc acgatagctg aaatgatgca
    52801 tggaaatccg cgtgctgttg gcaggccgcc atcggcttgc cgccccccgc tatgcgctgg
    52861 cgtctacgga ctcgtcaagt gagggagcgc ggacggggtg ccgcggtcga gggcggtaga
    52921 agtcgagggc ggtagaagaa gcgttcaggc tggcgaaaga tcaggaccct tgccttgccc
    52981 ttgcgcgccg gcgggggcgg tcatagtcct ctatgatgta acgatgcatc gtctcgagcg
    53041 tcgcccgctc ctcgctcatc acccagccgc gcaccgagat gccagcctga tgcaccgtct
    53101 ccggatcacc agagacgagc ggatgccacc aggcgaggtc gccgccattg gccagcacgc
    53161 ggtaggcgca ggtctccggt agccaactca gcgtacgtgc tttttcgggt gtgagcgata
    53221 cgcaatcggg cacgtggctc tgccggtccg catagctcga gcagcggcat gttccgagat
    53281 cgagcagccg acaggcaagc cgggtacggt agatttcgcc cgtgtcctcg tcctcgagct
    53341 tcaacaggca gcagcggccg cacccgtcgc acagcgactc ccactcggcc tcgctcatct
    53401 cctcgagagt tttttgcttc cagaaaggtt tttctgcgtt atccactcgc ttctctcgcg
    53461 ttcgatcgtg gcccgatggt aagatggcgg cgaccctacg cgggcataac ccctcagtcg
    53521 cggcgaaaag gcattcgacg tgtacgactg gttcttcaag cagcgcggcc gagatcggct
    53581 catcaactgg ctgggtctgg atgcttggat cgactccaca ctggccgaga cgtgggagcg
    53641 gatcaaggac ggctatgacg cagcctcgag cttctttgcc cggtttcgcc tcacaggatg
    53701 gaggcggctg ctgaacgagg cagcctgcga ggcgctgacg ctggcgacgg gcggcttctt
    53761 catcatgtac ggactggccc tgccggcctt catggaggtg gaggacggaa aatggcttac
    53821 gacgggtcag tacagcgtca agttcctcga cgtgaacggc aacgagatcg gcaagcgcgg
    53881 catcaatctc gacgatgccg ttccgctcga ggaaatcccc gactacatga tcaaggcgac
    53941 gctggcgacc gaggacaggc gcttttacga gcacttcggc gtcgacgttt tcggcacgtt
    54001 ccgcgcgttc atcgaaaacc tgcgcgccaa cgatgtcgtg cagggcggat cgtccctcac
    54061 ccagcagctc gccaagaacc tgttcctgtc ctcggagcgc tccctccagc gcaagatcaa
    54121 ggaggctttc ctggcgctct ggctggaatc ccgattgacc aaggatgaaa tcctgaagct
    54181 ctacctcgac cgctcctaca tgggcggcgg cgctttcggc gtagaggcgg cggcccagtt
    54241 ctacttcggc aagtcgatac gggacgtcaa tctggccgag gccgcgatgc tcgccggcct
    54301 tttcaaggca ccgaccaagt atgcaccgca cgtggatctc gccgctgccc gcgcacgcgc
    54361 gaacgtcgtg ctcaacaacc ttgtcgaggc gggctactac accgcggggc aggtccagca
    54421 cgcgcggctc aaccccgctc gcgtcgtgga gacacgccat gccgatagcc ccgactggtt
    54481 cctcgactac gccttcgagg aagtccagcg cctgatggcg ggcaaagggc atttcaacct
    54541 gatcgcgcgg acgacggtcg acctgtcact ccaggaagcg gccgaaaagg ctctggtttc
    54601 ggcgcttcgg cagcgaggcc gcgccgtccg ggccgacgca ggagctttcg ttgtcacgga
    54661 gaccgacggt gccgtgcgcg ccctggtcgg cggccccgac tacggcgaga gccaattcaa
    54721 cagggccacc aaagcgcggc gccagcccgg atcctcgttc aagatctatg tctacgcgac
    54781 ggcgctcgag aacggctaca agccgaccac gatcgttcgc gatgcctccc gttcctgcgg
    54841 ccactggcat ccgcagaact acaacggtag ccacggcagc ggcgcacgta tgccgctgtg
    54901 gctggcgctc gccaaatcgc tcaacacgac ggctgccgag ctgtcgttcg cggtcggccg
    54961 cgagaaggtg atcgagatga cgcagcgcct cggcattcag ggcgtccaga agtcctgctc
    55021 gatggcgctc ggcgacacgg gcatcacgcc tctccagcat gtcggcggcg tcgcgacttt
    55081 cgcgaatggt ggccggctgg cgaagccgta cgccatcacg gagatataca ccgcgagcgg
    55141 tgagctggtt tacagccgcg agagggacga gccgccggcg ccccaggtca tatcccggga
    55201 agtcgtcgag caaatgaacc agatgttgtt caaggtggtg gaggagggca ccggccagcg
    55261 ggcaaagctc gatttcaccc atgtcgccgg caagactgga acgagcaccg gccccaagga
    55321 cgtgtggttc gtcggcttta ccggcaagta tgtcggcggt gtctggatcg gcagggatga
    55381 caacaggcct atgtccggcg gaacgaccgg cggtcagttg gcggcgccga tctggcagca
    55441 gttcatgtcc gtggcgcaca ccgatatgaa tattccgacc ataccgggtc tcagtccgca
    55501 ccccatccag gtggcggagc agcagcgcgt cgctgcgatg caacgcaaca aggtcgccgc
    55561 atccgagcca gcagcaggac cccgaagcca gtccctgatg tcggagcgga cgcgcaaggt
    55621 tctcgagcgg atcgtcaaat ccctgcgcac cgccagtggc gaccaaggaa cggaggagcc
    55681 agcccctggt gcgcctgcag ccgaccgccg cgccgacgca gcgcgctggt tcgaggatct
    55741 tccgatcagg ggacgtccgt gaccgtagcc gtcgcatctg aggcgcctgc ccgcttcgcg
    55801 cgtctaaggg ccaccttggg cgccatattg ggcttcatcg gcaacattgg gcttttcgcc
    55861 ggcatagccc tgatcggagg gatcgggtcg agctggtaca tgatcgaggt cggcagcccg
    55921 ctgaccacgg tcaaatcggg gccctgggtc tcttggccgg cggccggcac accgaccgcg
    55981 gatccctaca cgcgcgcccg ggtcgtccgg cacggcagtc tgccaatcag cggcaccgtc
    56041 gcccgcatat gggaggcacg tacagacaac acgggacagc ggctgcattc gagctgcgat
    56101 tatctggtga ccgggcgtgg tctcgatgac ggctggtgga cgatcgccgt ctatgatgac
    56161 aacggccatg tcattcccaa tcccgcggat cggcacgcgt tcaattccag cacgatcatg
    56221 cgaagtgcgg acggcagctt cgtggtctcg cttgctcgtg atgcgcggcc ggggaactgg
    56281 cttccgaccg gcaacgcggg ccggctgacg ttggtgctga cactgctcga ggcgggagac
    56341 gggctcggcg gagctggcct gccggagatc cagaaggtgg cgtgccgatg agctacggtt
    56401 ggcggaccgg cggattgggc accgccctcc tctgcggggt agtgggagcc ggaattgttc
    56461 acatcgtcgc caccttcgcg acgcctgggc tgctgcggcc aaacacctat gatcgcatca
    56521 cggagcatct gccggcaaat acgcttgtcg tgcttggaca gccggaggcc gattcacagc
    56581 tcatcccgta tcaggagccc gacatgcaga tcgccgtctg ccactatgac gtgagcggcg
    56641 gccccgtttc ggcgcgcgtc gtcctgccga gtagcggatg gacgcttgcg ctctactcgc
    56701 cagcgggaga caatttttac gtaatgccgg gccgcgacca gagggtgacg atggtcaacg
    56761 cgctgctgat gcttcatcag aacgaggatg cggctctgcc gctcagcgcc gggcagactg
    56821 cccgtaatgc accgacctat atcgatgtcc ctgaaccgac cggcttgatg gtcattcgcg
    56881 ggccggtgaa gggcgaaagc tacaaggacg aggtcgcgaa catcctggcg cgcgcgagct
    56941 gcggtccggc gccgaatcgc ttgcagtcct catctgccgg gtaacctgta gccggatcaa
    57001 tcggggatca ggagaaagtc gcgccgtttg gccttgccgg tcgccctttc ctgatcgggg
    57061 cacgtcaccg tgcgctcgta gcgcacaccc gggaagatca cgatctcgca ccggcatggc
    57121 tgcgactccg cttcttctcg gcggtcagac cggtcgtgcc gggggctgaa ggacagaatg
    57181 gttcccattg atttttcttc ggcgtggcgg gatgcgaata cgtctgaggg ttgttcatta
    57241 tttcgaacgc aatcagaacg ggaaacattc gccgcgagtc aggttaagaa acaattaaga
    57301 tcagttatcg tcagcgccgg aagatgccag ccagggatcc aggaaatggc ggccctggcg
    57361 atcctccagc tccaggatcc atagatcgcc atcgaacgcg acctggcggg cgaggtaggc
    57421 gtcgacctcc gcctcggtcg cttccggtcc gtcgaggtgg gctatccact ggcgctcgaa
    57481 ctggccggta tcgaagccgg caggcgccgg gccgaacagg cgcgcggtcc catcgaggcg
    57541 attgaccttg atgaagatgg cgcccgcgtc gtcgtcccca tgccgcacga cggccgccgc
    57601 cacgccggcg gcggcgcagc tgcgcaggta ggcctttgcc cagatttcag ccttgagtct
    57661 catggaccca caggtaaaag gcaggtgggt ggagcgcaag ccggagtcgc gtggctatca
    57721 gcgcgccgag gcgcggcgct gccgggcgac ggccacgtcc cgcagcttca gcagcagcgc
    57781 accggaaacg cgtcccgaag gagtgaggtc atttttcgct tcgaaggctc ggatcgcgcg
    57841 taccgtttcc tcgcccatgc ggccgtcgac cttggaaatc ggataaccga ggccggccag
    57901 cgactcctgg actgatcgga tgatctcctg cgcctggggc tgcggctccc ctacgacctt
    57961 gccgctcgcg tcggccggaa cgccgaacag gatggatttc aggatctctt ccgaagggcg
    58021 cgctgtgagc ggcaggccat gatcatgctc gtaagccatc acggcggcgc gtgtcacggg
    58081 atgaatgagc ccatccgcgg gccccggatc gtagccgcgg agcgccagtt cacgctggac
    58141 cgcgcgcaca acgtcggcgt cgatctcagg ctccggaatg acgctggcca cgctcggaat
    58201 ttcgacggtc gggtgggctt ttcccggctc gatcagcgcc ggaggagggg gacgcctggc
    58261 ggtttcgccg agccgctgcg tgcgggcgag aatgtccgcg cgggtaccct gctgaaggta
    58321 catcacgttc caccacacgc cggcacacgc ggcaacgcag gcgcacagca cgattttcgc
    58381 ccgccgagct agcatgtatc cgaccctggg gctggaacct ctgaggattg acgacactca
    58441 gggttaatat cgggaatgca tcgttaagat tggctgaacg ctcgcgtggt gcggcaatcc
    58501 gggcaggcat tggccttgca agcgcggcag ccaattgcct atttcatgaa tgcagcgctg
    58561 aggccgagac gggtgcggcc ggcggggagc cagaatgtcc atcacagaca tcgcagacat
    58621 acgatccgat ttcgccctgc tcgacgagtg ggaggatcgc tacaggtatg tcatcgagct
    58681 tggccgctcc ttgccgccgc tggacgagtc tgcccgcacc gatgccaaca aggtgcgtgg
    58741 ctgcaccagc caggtctggc tgataaccga ggtcgtgccg ccggccgacg ggacggggca
    58801 gccgcggctg cgttttcagg gcgacagcga tgcgcatatc gtgcgcgggc tggtcgccat
    58861 cttgttctcg atctaccaga acaagacgcc cgaggagatt ctcgcaatcg atgcccgcgg
    58921 gatcctggcc gagctcgggc tccaggagca cctcacgccg cagcgctcca acggcttcgc
    58981 ctcgatggtc gaacgtattc gggcggacgc ccgcaaagcc gccgaaggcg ccctccgtac
    59041 caactgatcc atccgaattg aaggaaacgg acctggcagt cgggctagcg caacagccca
    59101 tccaggatga agccccggtg cggcttgggt gctgcgcctg cgggtttgcc ctgaccggag
    59161 tcggcctgag cggtcgtgtc cgctttcggg ctcggcgggg cttcgagaga gccgcgtacg
    59221 ccgcaaccgg caacagccag agctgttgcc acagatatgc tcagcacgaa tgctctactc
    59281 attctcgccc gcccaccttc tggcccgcga ttcgcaaacg aaactcgagc cgtaacaggc
    59341 ccatacggcc ccggcgcccg actctagacc ggccgagccc cgccgaccaa tgtgatggtg
    59401 gccggcggca gcattttgtc gggaccctgt ggcccaaggc ccatagttcc gcagcggagc
    59461 gctacgtgag gctggcgcag aaccgctgga tgcgcctgca ggcctcctcc aacgcgtcgg
    59521 tggacgtcgc gtaggaaatc cggaagaacg gggacatcgc aaaggcagcg ccgtggacgc
    59581 aggccacgcc ttcctcgtcc aggagcgccg tgacgaaatc ctcgtcgttg ttgatcttga
    59641 cgcctttcgg ggtcgtcttg ccgatcagct cctcgcacga ggggtagacg tagaaggcgc
    59701 cttccggctt cgggcacctg atgccgcggg cctgattgag catcgacacc acgaggtcgc
    59761 gccgctgcac gaagaccttg ttgttccgct cgatgaagtc ctgcgggccg ttcagcgcct
    59821 cgacggcagc ccattgcgtg atcgagttcg ggttggaggt cgactgcgac tgcagcttgc
    59881 gcattgcatt gatgagcggc tcgggccccg cgccgtagcc gaggcgccag ccggtcatgc
    59941 aataggcctt ggagaggccg ttcagcgtta acgagcgctc gtagagaccg ggctcgacct
    60001 gtgccggcgt ccagaacgta tggccgtcgt agagcaggtg ctcgtacatg tcgtcagaca
    60061 ggacccagac gtggcgatgc cgcatcagca cgtcggtcag cgccttcagc tcctccttgg
    60121 agtaggcggc tccggtcggg ttgctgggcg cgttgaagat gaaccacttg gtctgcggcg
    60181 tgatcgcctt ctcgagggtt tcgggctgca gcttgtagcc atcctcgata cggcagggcg
    60241 cgaacacggg cttgccgcca cccagaagca cgatgtcggc gtaggacacc cagcacggcg
    60301 cgggaatgac gacctcgtcg cccgggttga gcgtggccat cagcgcattg tagagcacct
    60361 gcttgccgcc cgtgccgacc gagacctgcg aggtcttgta ctcgagcccg ttctcgcgct
    60421 tgaacttcgc cacgatcgcc gccttgagct cggggatgcc atcgacgtcc gtgtacttcg
    60481 tcttcccctc gttcatcgcc cggatggcgg cttccttgat gttgacgggt gtgtcgaaat
    60541 ccggctcgcc ggccgagagc gaaatgatgt cccgaccctg ggctttgagc tgccgcgcct
    60601 tggtcgagac cgcaatggtt gcggacggct ggatccggtt caggctgtca gcgaaaaagc
    60661 ccatggttgc ctcacgatcg aagtcctgcg gaagatgtat tgcgcttgaa actgcggctt
    60721 gcgtaagcgc gcaagtgtac taggcgcgct ggcctgcggg agcaagcgcg tactgcctct
    60781 ttagcaggtc cgaagtatcg ggcccgccaa tcaccagcct ttgaacggcg tctcagccac
    60841 cggggtgagc ggtggcttgc gctcggcgta ggccttgagg ttgtcgacca cgagctggcc
    60901 catcttgttg cgcgtatgga cggaggccga gcccacgtgt ggcagcagga ccgtgttcgg
    60961 caacgtcagc agctcgggat cgatattggg ctcgttcacg aagacatcga gacccgctgc
    61021 caggatcgtg ccgtctctca gcgccttcat gagagccgcc tcgtcgacgc agcttccacg
    61081 ggccatgttg atcaaaacac cgcgcggccc gagcgcctcg agcacggcag cattgatgat
    61141 gcacttcgtt tctggtccac cgggcgtgat gacgatgagg gtgtcgacgg ccttcgcgag
    61201 ctcgacggcg ttgggatagt acgtataggg caggtcggtc cgccgccggc gggtgcaata
    61261 cgagatcggc aaaccgaatg cctcgaggcg acgcgcgatg gccattccga tgcgccccat
    61321 gccgaggatg ccgacgctgc ggtcccgcag actggctggc gtcaggcgat agtcgccgtg
    61381 ctttgcccat ttgccctcgc gcagccagcg ctccgcttgg gcgagctccc gcacggtcat
    61441 gagcagaagt ccgatggtgg tatcagcgac ctcctcggtc agcacgtccg gcgtgttggt
    61501 gacgatgatg ccgcgcttgc ccgctgccgg cgcatcgacg ctgtcatagc cgacgccgaa
    61561 attgccgatg atcctgagct tgggcagccg ccccatcaaa gcgtcgtcga ccttcacgcc
    61621 ggtgtggccg cctgtgcaga tcgcctcgat gctctcgccg accctggcga ccaccgcttc
    61681 caggtccgcc ggctctttca gccggtgcac atcgaacgtc tggtcgagct gctcctcgat
    61741 cagcgccatc atgtgaccgg tcatcagcaa ggcaggtctg gccatcgtca gtctccagcg
    61801 ggtgttcgtt cttcgagacg ggtcgtacca cttgatggcg agtccttcaa cgatcccgcc
    61861 gcattgccaa gagcgaccga tcgcgggaca agcggcgcat gagcgaaccg caatacgacg
    61921 tcgcgatcat cggcgggggc cataacgggc ttacctgcgc ggcctatctc gccggggcgg
    61981 ggctgcgcgt catcgtgctg gaaaagaacg atgtggtcgg cggcgcggcg gtgaccgagg
    62041 agttccatcc ggggttccgc aactcggtcg cctcctacac cgtgagcctg ctcaacccga
    62101 aggtgatcgc cgatctcgag ctcgagaagc acgggctgaa aatcctcgag cgtacagcag
    62161 cgaacttctg gcccatcgat gccgagcgcg gtctgctgat gccctacggc ctcgccaatc
    62221 ggcagcgcgc ggttgcggag ttctcggcgc gcgatgccga acggctcccg gcctacgacg
    62281 cggcgctcga tcgggcggcg aatgtgctgc gcgagctcgt gctgcgcacg ccaccgaatg
    62341 ccggcggcgg gatcgtggag ctggtcaagg cagccggcct cggccgccgc ctgctcggac
    62401 tgtcccttaa ggatcggcgt ctgctggtgg acctcttcac caagagtgcc gccgattacc
    62461 tcgcctcgtg gttcgagagc gagattatcc agggcgcatt tgccttcgat ggcatcgtcg
    62521 gggcctatgc ctcgcccttt acgccgggaa cagcgtacgt gctgctccat cactgcttcg
    62581 gcgaggtcaa cggcaagccc ggcgtctggg gtcacgccgt cggcggcatg ggcgcgatca
    62641 cgcaagcgat gtgcgcttcg gctcaggctc gcggcgcggt gatccggaca ggggcaaccg
    62701 tcgcgaaaat caactgtgag cacgaccgcg ccaccggcgt ggtcctgcaa tccggcgagg
    62761 aaatcagggc gcgtgtcgtt gccgccaatg tgccgccgaa actgctgttc cgcgatctcg
    62821 tgcccgagac tgccgtcgcg ccggagctgc gggcccgttt cacgcacatg aagacaggct
    62881 ccggcacgtt caggatgaac gtcgcgctgt cggagctgcc ggacttcacc tgccgccctg
    62941 gaaaagaccc ccagccgcat cacggctccg gcatcatcat cggcccgacg gtctcgtacc
    63001 tggaacgcgc ctatctggat gcgcgccttt acggctggtc ccgcgagccg gtcgtcgaaa
    63061 tgctgatccc gaccacgctc gacccggcgc tggctccgcc gggcaagcac gtcgcgagcc
    63121 ttttcgtgca gcacgtcgcg ccgcgtctgc cggacggccg gtcctgggat gatgccaagg
    63181 atgaatttgc tgatctcgtg atcgagaccg tgacccggca tgccccgaat ttcaaggcga
    63241 gcatcatcgc ccggcaggtc ctgtcgccgc tcgatcttga gcggcgtttc ggcatggtcg
    63301 acggcgacat cttccacggc cagctgtcgc tggatcagct cttctcgacg cgccccgtgc
    63361 tgggccacgc cgattaccgc atgccggttg caggtctcta tctctgcggc tccggcgccc
    63421 atccgggagg cggcgtcacc ggggctcccg gccacaacgc cgcccgcgag atcttgcgcg
    63481 atctcaaggg ttggttcggc cgcgcgggat tcgggtaaag cggcgcaggc atccgcctgt
    63541 tcgagctgac atttcctgtc ccatcgggaa cagcgcgcaa agcacgaatg cagcatcctc
    63601 tctccaggtt cagtcgagaa gggccccgca gcgggctcgg caaaaaagca ctgtggagag
    63661 gatcatgtac ggacagatca ccaagttccg ccctgagatc gggaccgggg tcattcgcgc
    63721 cgaggacggc cgcaaatacc ggtttgtcgg ctcggagctc atgaacagag ggcgcgagct
    63781 tgtcggccag ggcgtcgact tcgtcctcgt cgagcggcgt ccgcgagaga tcatcgtgct
    63841 cagcggctcc ccctggaccg cattcggcgc caacccgcgc cgccccaatt gacgatcagc
    63901 cccgaaggag tgattgccat ggctatcacc tgggacccca ccgatgtccc aaccgtcgcc
    63961 gccgaggacc tcgctgcccc gatgcgccgg ctgatcgcgg aaggccgcgg gctcgtgctt
    64021 ctgcgcgggc tcactgcaga ggatgcgaag atcgtcgagg agtgcctgcg ccagcgtctc
    64081 ggcaaagagc cttcggtcga gctggcggcg ctgatgcgct tcagggcgct cgtggatgtt
    64141 tttgcctgtc gtcgccttag cgagttgcta ctggagcgag gccatgaggt gatcgcacca
    64201 tcgctgcagt gcgcggctca aatgaggctg agcctgaagt ggggcttcaa cccgcggaag
    64261 ttccatcgag tcgtcaccga gctgctcgat gggggcgatg cgggcatcta cttcgtgagt
    64321 cgtgagttgc tcgacgctgc ctgacggccg cacctactgc gacgcaaacc aggcgaccgc
    64381 caggatcgcc aggagaaatg cagcgattgc cagccatgga gctacgccgc gcgtttgcac
    64441 gctgactacc cgggcgggtg gcagggcacg tgcaagccgc ccgatctgca actcgcgttc
    64501 atgggcctct gcaatgactt ccgggctcag cggcgtgccc gcagcctcct cgtcggtgcc
    64561 gagcggggcg gccgccgggt ctgccgctgc aaccttgtcg ccggtcaggc cccgatcgat
    64621 atcgcccctg agttgggcgg aagttgattt tcgcttactc atcacgccgt ccaggtggtc
    64681 tgtcgcacct ggacgaccaa cggcgcatca gccgccaagt tccgtgacgc ccgctccgaa
    64741 cctcgcttgc gctcgcgggc agattcggag ccttgcgatc caggagagat tccggtctca
    64801 ccagtgccag ggccggatcc tgccgtgata gatccagtgg aactcgcgat tgagccacgg
    64861 cttcggatag ccccaggctt tccagtaggg cggttgatag ggccgcggca cgcagacggt
    64921 cttgccgcgg cagatcggcc gccagtagcc ggcgtcgtaa tagggatagt atccgcgggg
    64981 ctcatacctg taggcgtagg gatcggcgac gaaatagccg ctcaccgtcc tcggccgggg
    65041 ggcaggatag taggcgggtc ggtaatcccg gccctcatcc caggcccgat aaccttccgc
    65101 gtaggacggc tgtgctgcca tggaggcgca ggcgagggcg gcaaccaggc cgcccaacag
    65161 gacgagtgaa cgcatgacgg gtctcctcgc gcggcaatcg cggaccgttt ccgatcggat
    65221 tatatgtact tgtaaacgat gctgccgtcg cgccgcatcg accggtaggc tttatgaagt
    65281 ctgaagcccg acccccgcga cggcctgcat ctggccagat cccccatccc gggctataag
    65341 cgcgctcaat agcaatcgcg ccggccggca tagctcagtt ggtagagcaa ccgccttgta
    65401 agcggtaggt cgggggttca agtccctctg ccggcaccag ctcccaatag tgcctgactg
    65461 accaggagcg ccgagcggct gacggcatta gaaggcgtga gcttgggaag ccttggccac
    65521 tgccacggtt gccggaaggg ccagcggcgc aatcacctca gccgaagcag atcgctcttc
    65581 aagaccctgc cggcggttct cccttgcgga gagccgttga aattgctgat cttggctagc
    65641 tcaacgtcgg cttactcttc acaaaacggc gataaccaaa ggcaggcccg tgttcatccg
    65701 tgttgtagat gtattgcaga ctgtttcctt ccaaagtaaa gctcttggag cagccgatgc
    65761 cagactaggg gccgcccaat acaatcataa attgagaaat cgtctgcgca ttgtctggaa
    65821 ttccgcagtg ggaggagcgc ggaatcatga ttgacatact catcgcggaa gacgagccag
    65881 ccatcctcga atccttgaat ttcatactgg gtcgctgtgg ttggatcgtc gactcggtga
    65941 gagacggtga ggaggcgctc gatgccgtcc gccgtcacaa gccgcgcttg ctgctgctcg
    66001 acatcatgct gcccaagcgc agcggcctcg atgtcctcaa gtcaatgcgc gccgatcccc
    66061 gcctgaacca cattccggtc ctgatcctca ccgcgcgtgg ccagcagcat gaccgccaac
    66121 tggcgcagga gctcaaagcc gacagcttcg tcaccaagcc gtattcgaat gaagaagtca
    66181 tccgggaggt gcggcgcctt ctggatcggg gctgagacag gtttgcgatg gccaagcgcc
    66241 cgttcgtcaa ggaagaagat gcgcgaaaag cggctgaggc cgtcttcttt gcccagatct
    66301 gcggggcctt gctcctgctt cctccgatca tcaatctgtt caacaagcgg ttatcgctgt
    66361 tcggcgtgcc gctcgaagtg ctctatctct ttctggtctg gatagtgctg atcgcgagtg
    66421 cggtcgtcat ttcagccagc gtgccgaggg cgtctgccga tgaacagctg ggcgaggaac
    66481 cagcgcagcc gacgaacgag tccggaaaag agggtggata atgctctctc cggatttcgt
    66541 cattctgacg gccattgcct acgttggttt cctgttcatc ctggcctatg tttcggacgc
    66601 gcgcgccaaa cgcggcaagt cgggcatctt ccgctccccc ttcgtctaca cgctctcgat
    66661 ctcggtctat tgcacgagct ggacgttcta cggcgcggtc ggcacggcag cgcgcgacgg
    66721 cctcgaattc cttgccatct atctcgggcc gacgctggtc ttcgtcggct ggtggctcat
    66781 tctgcgcaag ctggttcgga tcagccactc gcaacgcatc acctcggtgg ccgacctcct
    66841 gtcgtcgcgt tttggcaagt cgagccggct cgccgtcctg gtgacgctga tcgccgttgt
    66901 cagcgtgacc ccctacatcg cgcttcagct caaggcgatt accagctcga ttcaagccgt
    66961 ctccagcgcg acaggctcga gcggcgggac gctcgtcgga ttggatgacg taaggctcgc
    67021 cttcggcatc gcgacggtga tggcgatctt caccatcctt ttcggcaccc gcaacgtcga
    67081 cgcgcgagag cagcatcacg gcgtggttgc ggcgatcgct ttcgaggcgg cggtgaagct
    67141 ggtcgcattc atcgctgtcg gcatcttcgt ggtctacggt ctggggggcg ggttcgagaa
    67201 ggtctacgcc cgcgctctgg aggccggcat agacattcac gagaagggcg acttcgggac
    67261 gcgctgggtt gccgtgcttg tcctttcctc cgctgccgtc atctgcctgc cgcgccagtt
    67321 ccagatcacg atcgtggaga actccgacga gaaccacctg cgcaccgccg gctgggcgtt
    67381 cccgacctac ctgttcctga tgagcgtgtt cactttgccg atcgcgctct atgggctggc
    67441 gacgcggcct gcggactcaa atcccgacat gttcatcctg gcgctgccca tgtccgcagg
    67501 aaatgacgcg ttggcgctct tcgccttcat cggcggcttt tccagcgcga cctcgatgat
    67561 catcgtggcg tcgatcgctt tgtccatcat ggtctcgaac catgtggtca tgccgatcgc
    67621 gctccgccgg tccaacaacc tcgaagcgac cggggacaga gatattgccc agctcctttt
    67681 gcgcagccgc cggatctcga tcgcaggcat gctgtttctc gggttcgtct acttctggct
    67741 cacgggcgac tccggcgccc tggcacccat cgggttgatc tcgttcgctg gggtcgcgca
    67801 attcctgcct tcgatgctcg ctgcgctcta ttggcggcat gcaacggtgc atggcgcgat
    67861 cagcggcacg ctggtcggct tcatcacctg ggcgtggacg acctttctgc ccagcttcca
    67921 atcgaccagc cctgccatag cgacgctgat ggcggaaggg ccctggggca tctccattct
    67981 gcggccgcag gctctgttcg ggatggaggg cttcgatccg ctcgtccact cggtgttctg
    68041 gaccctcctg ttcaatgcgg gaacgctggt gtgcgtctcg ctggcaacca gccagagcgc
    68101 gctggagcgg atccaggcaa cgctgttcgt cgacattttc agacgtcatc ccggcagcca
    68161 gccgcagatc ctgcgtgggt cggcgacgac cgacgagctg ttcttcgtgg ccgaacgtgt
    68221 tctgggctgg cagcgggcga aggagctttt cgaaacctcc gacgtcccgc acaggggtgc
    68281 atcactcccg ttcggggttt cgtctgcatt cattaccaag ctggagcggg agatggcggg
    68341 ctcgatcggt gccgcctccg cccacgttct gctgtccaag gtcgtggccg gcgattccgt
    68401 ttccctcgag gaaatgatgc gcatcgccga cgaaacccag caggtcatcg aatactcgca
    68461 tgagctcgag cagaaatcga tcgagcttcg ctccacggca cagcaattgc gcgaggccaa
    68521 cagcctgctt caggaattgc accgtcagaa ggacgacttc ctctcccagg tgagtcatga
    68581 ggtacgcacg cccatgacgt ccattcgttc tttttcagaa attcttttga tggatgaaga
    68641 tttgaatgct gatcagaggc agcgcttcgt ttcgatcatt caccaggaaa gcgtgcgcct
    68701 gaccaagctc ctggacgaaa tcctcgacct cagcgccctc gagcacgggg agagcgactg
    68761 ggagaacact tcggtcgacg cggagcaggt tctcgaccgg gcgatcgccg tgtgcgaggc
    68821 gctggctcgc cagaaatccg tgaccgtcga gagcagatcg cgcgccaggg caggtcgggt
    68881 ctacgcgaac gccgaccggc tgtcgcaggt gttcatcaat atcattgcca acgccatttc
    68941 gcacaacaat gccgaggatc ccaaggtcca gatcacatcg tggaccacgg ccgaaaccta
    69001 tgtcgtcgag atttgcgaca acgggccggg aattccgaaa gagtaccgga gaaagatttt
    69061 cgacaagttc gttcgcgcga gccccgcagc ggacgtatcg acgcccacct cgggattggg
    69121 attgggtctg aacatcagct acaccatcgt gaagaaaatg aatggggctc tcgagctggt
    69181 cgatgggcat ctgccgggcg cgtgttttcg cattgtcctc ccgctcgagg gagtcggagt
    69241 gaaagccgag agagcaagag tctcgggata ggaatgaact ttcggttcaa tccgatcttt
    69301 tcggtttcag aggcagtgcc ctcactttga aaatggctcg tgcgcgcgct cgaacggccc
    69361 ggcctcagat gatcgtcgcc ggggccggct cctgcctatg ctcgcaatcc tggcggctgc
    69421 agaggaggca ggatgggccg accaggacac gattgcgtga atcggcgaga tcgagaccct
    69481 gcccgtagac gatgcggtct gcatgcagga tgtcgcaggc aagcatgatg ctgaacgtga
    69541 tgggctgttc ctggtaggct gcaatctgtt tcgtcaccgc tttcgcaatc agcagatagc
    69601 gcgcgccatt cgtgaattca gagatttgcc ggacgacctt ccccgtgctc ggcgccgaat
    69661 aaatcggcca cagcggacag ccgtggccaa ggatcggcag gatgaggccg ggaatcggaa
    69721 agcgcttggt cagcctcccg gccggatcgg cacggaggaa gccaaacggc acgccctcgg
    69781 cacctttcct ccgcaaggtc acgaaccgat gggcgacctg ttcgaagctt gcgtcgtaaa
    69841 tatggctgag caggtcgatg tcatagcgcc gggactcggc atcgttgaga aaggcatcat
    69901 agggcatcac catcgcgccg gcgacgtagg acgacagcgc ccggcgcgcc agctgcgagg
    69961 cttccttggt cgtcagtgag agccttttga cctgatcctc cagcagctca ggcgcagcat
    70021 gctcggcata aatgcggcac atctggaaac gccgcgtggc gggggtcgtc gaaacccgga
    70081 accagagggt acgagactga ggatcgaaac tgtgctgacc ggtgaacgcc tgcccggaac
    70141 gacactcgat cccgaaccgc cgtctcaggg catcgcccaa atccggttcg ctgatgatgc
    70201 cgttccgctc cacctcgcgc cgcaggaaat cggcgtttat ctccagctca gggaaatgat
    70261 tgttggcagc gattatcgca tcctcgacct ctcgcaacgg cgagacgatc ctctgccgcg
    70321 aggcggtctg gtcgaagtac gagacgagat tcttgagaat gcccgtgaga tcctccgctt
    70381 cgccatttat gtttcggagg aaccggtctc gatctgcttc cgtcagatcc ggcacgcccg
    70441 acagaatttc ggcactggat cggatgccgg caatgcggtt gagcacctga tgcagcatct
    70501 cggacaggag cggatcggac ttgagccgat tcgcataagc ctccatgccc gcgacagaat
    70561 caatataggc ccgatgcagc cgcgccatgg cgtgcgcagc ctcggggtga cgggcgacga
    70621 gatcccggat gctcgcagcg tcgatctcga ggccattgag gagcggctcc gacagcagct
    70681 cctcgagggc atggatcgtc cgctgctcgg catcccccga tagccggtcg acatcgaggt
    70741 tcagccgctc ggcgatgcgc agcagaagcc gtccgccgac ctctctctta ttgttctcga
    70801 tgagattcag atagctcggc gagattccga ctgctcgggc gagcgccgcc tgcgagagat
    70861 tgagcgcatt tcttcttctg cgaatctgaa ttccgacggg agcgcgcatc gctgccggag
    70921 gctttctgtc tggtcgtcgt ctctgtcaag aattgacaaa attgacttta ctaatcgcgg
    70981 cgagtttaca aaaaacgccg gactaatcaa gcacgtattg tgttttactt cttttgagca
    71041 acactcatag gagggaacag gagccgttgc tgcgctcaaa ggaggaagcg agatgtccaa
    71101 gtcgaaagtc agcaggcgat cggtgctgaa gggcgctgcc ggattggcag cgataacggg
    71161 gtcgccgtac attttcagca agaccgcctg ggcagagaac tattgcaacg cccccggcaa
    71221 gaccgcgatt ttcggcttca atgtcccgca gaccggttcc tacacggagg aaggtctcga
    71281 ccagctcaag ggctatcagc tcgctgtcaa gcacatcaac ggcgagggcg acggcgggct
    71341 cctcaacaca ctgaagccca gggccctcaa gggcaacggc atcctcggca aaaaggtcga
    71401 gtacgttacc ggcgacaccc agacgaacac cgacgtcgcc cgcgacaacg cacgtcgcat
    71461 gatcgagcgc gacaaggcca tcatggtgac gggttgcgtc acatccgccg aggcgattgc
    71521 ccagcaggcc ctgtgtcagg aaaagggcgt catctacatg tcgggcctga cgcattccaa
    71581 cgatacgacg ggtaaggaca agcgccgtta cggcttcagg catttcttca atgcctacca
    71641 gtcgggcgtt gccattgcgc ctatcctggc caaggaatac ggcaaagatc gccgcgctta
    71701 ccacctcacg gccgactata cctggggttg gacgcaggaa gagtccatca agaatgcgac
    71761 cgaagcgctc ggctggcaga ccgttgcgac cgttcgcacg ccgctcggcc tcggtgacta
    71821 ctcgcagtat ctcaccccgg tcctgaattc cggcgccgac gtcctcattc tcaaccacta
    71881 cggccacgac atggtgaact ctctgccgca ggcggtgcag ttcggccttc gtgaccgtca
    71941 ggccaacggg aagaacttcg agatcatcgt cccgatgttc tccgaactga tggccatcgg
    72001 cgccggcgat gcggtgaagg gcatcatcgg gacctcgaac tggcactggg cgctcgatga
    72061 cgaagggacc aaggcattca ccaagtccta tggtgaggaa tatggtgcgc caccttcgca
    72121 gtcgtcgcac acgggctacg tgcaggcgat tctctatgcg gacgccgttg agcgtgccgg
    72181 caccttctat ccgcctgagg tcatcaaggc tttggaaggc tttgaattcg acggcatggg
    72241 caacggcccc acgctctacc gcgacagcga tcaccagtgc ttcaaggaag ttctggtcgt
    72301 ccgcggaaac gagaatccga gcagcaagtt cgacgtgctg caggtgatcg gaaagacatc
    72361 ccgcgaggaa gtcacatacg atccagccat tttcggtggc gagctcggac cgtacgagcc
    72421 caaccagtgc tgaattgaag gcgcccggcg tttcacaaaa atgccgggcg cctcttttca
    72481 ggccactttc gtttttcgga tctgtttccg ccggggaaac tcatggatgc gttcttggcg
    72541 cagctgctca atggcctcga taagggcggc gcctacgcgc tcatcgcgct cggcctcacg
    72601 ctcgtcttcg gcacactggg cgtcgtcaac ttcgcgcatg gcgcgttgtt catgctcggc
    72661 tgcttctgtg cggtcacttt ccgctatctg atcaccattc agacggtcac gatcgacccg
    72721 acgcggcttt ccccttgggg gcagcctgtc gaaatccgca ctccgctggt gcaaaactgg
    72781 ttcggcgact tcggggccgt gctggtcgac tactcggtgc cgatttcgat cctgctggcg
    72841 atccccgtca tgctcgccat cggcattgcc atggagcgtg gcctgatcaa gcacttctac
    72901 aagcgcagcc atgcggaaca gatcctcgtc acgttcggcc tggccatcgt gcttcaggaa
    72961 atcgtgaaga gctttttcgg cgccaatccg attccgcagc ccattccgtc tgctctgcgt
    73021 agcgcggccg atatcggatc atggctgggg atggcacctc atacgctgac gtatccgtgg
    73081 tggcgcctga tttatcttct cttttctctg gccgtcatcg gtggggtctt tgcgttcctc
    73141 cacttcacca cgttcggcat ggtcgtgcgc gccggtatgg cggaccgcga gactgtggag
    73201 ctgctcggga tcgacgtgga ccgtcgtttc acgatcgtat tcggcttagc ggccgttgtg
    73261 gccggcgtgg ccggtgtcat gtacacgccg ctgctgccac cgcattacca tctcggaatg
    73321 gacttcctgg tcttaagttt cgtcgtggtg gtcgtcggcg gcatgggctc ccttccgggt
    73381 gccgtggccg caggttttct tctcggaatt ctgcaatcct tcgcgtcgat gacagaggtg
    73441 aagttgctca tccccggcat tgaccaggtc atcatctatc tcgtggcagt gcttgtcctg
    73501 ttgacccgcc cacgcgggct gatgggtcgc cgcggcatca tggagagctg agccgccatg
    73561 ttttcactgt cgcgcaaaga tttgttgtcg ctcgggatct tttcggcggt cgtgctgacg
    73621 atgccgatat ggctgacacc attcggcgcc gcctacccgg gcctgatgca gaaattcgcc
    73681 atctacgcgg tcttcgcaat cggcttcaat atcctgttcg gattgaccgg gtacctgagc
    73741 ttcgggcacg ccgcgttcct gggggtgggg tcctacacga ccgtctggtc gttcaagctc
    73801 ctgagcatgg acatcatacc agccatcgtc ctcgccgtgc tgacggcggg cgcattctcc
    73861 gctgcgattg gctacttctg tttgaggcgc tccggaatct actttgccat tctgacgctc
    73921 gcttttgcgc agatgtgcta cagtctcgct tattccgtgc tgacaccgat cacgaacggc
    73981 gagaccggac tcctcctggc gcgcaatgat ccccgcatca tcgatgcggc gctcggcctg
    74041 aaggaggcga gcgcgggatt gccgaagccg agccttcttg gattcgaggt tggtgcttac
    74101 ttcggcttct acgtctgcgc gttcgtgttg atcgtctcat tctacatctc gcagaagatc
    74161 ttcgcctcgc ctttcgggtt ggcgcttcgg gggatcaagt cgaaccagac ccgcatgaac
    74221 tacaccgggt tcaataccaa accctacatg ttggccgcgt tcatcatctc cggcatgttc
    74281 gccgggctgg cgggcgcact actctccgtg accgacccgc ttgccggtgc ggagcgcatg
    74341 cggtggaccg aatccggcaa tgtcgtcctg atgacgatcc tgggcggagt cggcacgcta
    74401 ttcggcccgg tgctcggtgc ttggctgatc aagtacttcg aaaacatttt ctcggccatc
    74461 aacgagcaga tgctgcatag cttcttctcg ttcctccctg gcggcctcga ggatgtgttc
    74521 gtcatggttc tctccaagtt cgtcggagag ggctggcatc tgaccctcgg cctgatgttc
    74581 gtgctcatcg tggtcttcct gccgggcggg atcgtcgagg gtgtgcagcg cctttatcac
    74641 caatggcagc agcgccattc gaaacccgag gaagcgctca gcgctcaacc tgcggagtga
    74701 cttcgatgct caagaattcc gcaaatgcgg ttctgcatgt tgccgacgtg aagaaaaact
    74761 tcggcggcct ccaggctctg tcggacatag acctccagtt cgaagaaggc cagacgcacg
    74821 ccatcatcgg gccgaacggt gccggcaaat cgaccctgct caacgtcatc gtgggccgca
    74881 tcccgccgtc cagcggcgcc gtgattttcg acgggacagt gctgaccggc aaggcgccgc
    74941 accagatcaa ccagctcggc atttcacgcg tgtttcagac gcccgagatc ttctccgacc
    75001 tgcccgtggt gcagaacgtc atggctgcgg cgttcgcgcg gcgggacgga gcattcaaga
    75061 taaacctgtt ccgcagggtc gagggggaga aggaaatccg cgagcaggcg gaggaggtgc
    75121 tgcgcgacgt tggtctctat gagcagcgcg atgcgcactc aggatcgctg tcgcgcggcg
    75181 acaagagacg cctggagctc gccatgtgcc tgatccagaa gccccgtctc ctgctcctgg
    75241 acgagccgac ggccggcatg tcgcggcacg acacgaacaa gaccatagat ctcctccgga
    75301 agataaaatc ccgcggaatg acgaaggtga tcatcgagca cgacatgcac gtcgtcttct
    75361 cgctggcaga ccggataacc gtgctggcgc atgggcggat catcgcctcg gggcagccgg
    75421 cggagataaa ggatgatccg cgtgtgaagg aagcctactt ggggagtgct ctggaatgac
    75481 cgttgcagcc attgaaaggc cccctgcggt cacaaccgcc atcgatgccg ccggcgactt
    75541 cttcgcgatg cgcaatgtcc atgcgtatta cggcgagagc tacatcgtgc agggggtgtc
    75601 gctggagctg ggcaaaggcc aaatcctggc gctcctcggc agaaacggtg ccggcaagac
    75661 gtcgaccctg cgtacgatcg cccggaccgg ccagccggaa ttgcgtgcgg gcgaaatctg
    75721 gctcgaaggt aagccgatcc acacgatgaa gtcgttccag gcagcgcaga acgggattca
    75781 gctcgttccc gaggatcggc ggatcatccc tggcatgacg gtcgaggaaa acctgatcct
    75841 cgcccaggtc gccccgggcc acggctggga tctcgaaaag gtctatacgc attttccgcg
    75901 cctggcggag cggcgaaacc aggaaggagt caccctttcc gggggggagc agcagatgct
    75961 ggcagtcgca cgcgcgctgt cacgagaggt caagctcctg ttgctcgacg agccttacga
    76021 aggattggct ccagtcatcg tgcaggaaat cgagcggatt ctgtacggca tccgggatct
    76081 cggcatcacg accatcatcg tcgagcagaa cgccgtcgct gctttgcgga tgtccgatcg
    76141 cgccgccatt ctcgattcgg gcgagatcgt ctttacggga agtgcgaaag agctgctcga
    76201 caacccggag cttcgggacg aatacctcgc catctaggcg ggagcatatc gagcgcattt
    76261 ggcgggcaac ccgtctcgcg cggggctaga tgggggtcat ctcggttggg ccatgcggcc
    76321 aaacttccag gccgactgat ctcctttttc tgatttgcca gcgccggccg tccgaaaagt
    76381 aaggaagact gtcgggcggc ggctcgccga cagccagccg caggataagg acgctggcgg
    76441 caagggccgt tgcggggtgg actttgccgc aaggtgcagt catcctttcc cccgtcgcga
    76501 gcggttcggc aggcaaccgg cgttccgcat gcgacgccaa gggcgatgcc aggcagcaag
    76561 cagccggcga gatggcttcg agaggtcaac agttcgtctt cagtgtgcgg gagctgagcg
    76621 gcgcgctcgg cgatctcggt acgctgctgc ctctgatgct tggcgctatt gccgtggccg
    76681 gactggcgcc tgctcccgtg ctgctgggct tcggcctgtt ctatctggca accgccatcc
    76741 actaccggct gcccattcct gtgcagccca tgaaggccgt cgtcgcggtg atgctcacga
    76801 ccgagatcag ccccgcgacg ctcgcgtcga gcggtgtcct gatcggcttg acgctgctgg
    76861 tgctcgggtt gaccggatgg atcagctggc ttgctcgcct cgtgccccag tccgttcttg
    76921 ccgggctgca gctcgggctg ggcctggcgc tcgcgctcgt aagcctcgat ctgatggcga
    76981 cccagccggt cgttgcggcc gtgaccgccg gatccctcgt cgcactttta tttttgcccc
    77041 gctatccctc cgcgatcatc gcgctgacgg ttgccttggt cctgggatat ctcctcggcg
    77101 cccccggcat cgagctggca aatgcgactt ccgtttcgat cccggtcccg tcgctgccca
    77161 ccttcgacga agtggagcgc gctctttccg tgctcgttct accgcagctc cctttgacgt
    77221 tcaccaacgc catcctgctg acggcccttg tcgcgggtga ttacttcgag gaccgggcca
    77281 agcacgtgac gccagcgcgg ttgtctttga caagcggtct cgccaacctc cttctcacgc
    77341 cgttcggcgc tttgccgatg tgccatgggg caggggggct tgcagctcac taccgcttcg
    77401 gcgctcgcac cggcgcggca ccgctgagcc ttggccttgt cctcatcgca gctgcgttct
    77461 tgcccggcgc tggcggcttg gcggtgctcg ctgccatccc cgtcgccggg ctcggggccc
    77521 ttctgctgat cgcctcgggc gagctggcac ttaccaagcg gctcttcgat tcgaagccct
    77581 cctgctggcc ggtgatcgcc acgaccgccg ccgtcactct ttgggctgat cctttctggg
    77641 ggctgctggc aggctccggc gccgagctgg tcaggatggt ggctgttcgt gctttccgga
    77701 gagaggccaa gagttgagtt gcacgagggc agaggcggta agccgaattc ccgtcgttcc
    77761 gtccaggcga tccgcggcta gcacaggtgc aggtgggaag cttgacgcga cttctgagcc
    77821 gtagctaact cgaaaaggac agagctcttg ctgttgtgcc accaatggcc gaccaggcgc
    77881 ggagaaccaa gatgaatcaa ccgaagcgaa agaccgagct gcgccgcgac gcgggagggc
    77941 gtgccgcaca cgacgtcggt ggtttggatt tcggcccgat cgatcgcacc gagcacgatc
    78001 tggcgctctg ggaaaagcgg gtcgacgcca tgctggtcct gttgaccggc ccgaaaaaag
    78061 gcgcgttcaa ggtcgatgcc ctgcgccgcg tcatcgagga ttacggccag cagcaatacg
    78121 attcgaccgc ctactacgag aagtggatcc gggccatccg caacctgctc gtcgagcagg
    78181 aagtgctcac cagagccgaa gtcgagagca aggtcgcggc cgtcgaggcg gccatgcgtg
    78241 ccgccgggcg cgagctcgtc ggcggttccg tgccctggga tgcgatggag tcgccgatct
    78301 gatggagatt gctccgcgct tccggccggg cgatctggtc cgggtcgatg atagaatggc
    78361 gctcggccat tgccggacgc cttggtacct gcgcgggaaa acaggcgtca tcgccagcgt
    78421 gcacggcgcc tttcatgatc ccgagaaact tgcgtatcac aaaccgggac ttccgcggca
    78481 ggttctctac aaggtgcgct tcaggcaatc cgatctctgg gcgggatatg cggggagtcc
    78541 gcgagatcag ctcgaggccg acatttatga gcactggctc atcagggccg atggtgaggc
    78601 aaggagcgac agccatgccg ccgcatgacg accatgatca cgatcacgac cacgaccatg
    78661 cacatggcga cgggcaaccg catcggcacc cgccacaacg cgatcacgat gctgatccgg
    78721 cttcacctta cgaggtgctc gagcgggcga tccgcgagct gctgatcgaa aagggtgtgc
    78781 tgacggcgga tgagatcagc gcccaggtcg acctcatgga cagccgcacg ccggcgctcg
    78841 gggcgaaggt cgtcgcgcgc gcctgggtcg atccggccta caagacgcgc cttctggaaa
    78901 acacgcgcgc ggcgctggct gagctcggga tcgacatcgg caccctggcc gatttccgta
    78961 ccgtcgagaa cacgccggac gtgcacaatg tcgtggtctg cacgctctgc tcctgctatc
    79021 cgaagctgct gctgggcatt ccgccggcct ggtacaagag cttggcctat cgctcgcgcg
    79081 tggtggttga cccgcgaggc gtactcaagg agttcggcct cgtgcttcca gccgataccg
    79141 aggtgcgcgt ccatgattcg acggcggatt tgcgttatct cgtactgccg cgacggcctg
    79201 atgggactga cggctggagc gaagagcaac tcgcggagat cgtcacgcgc gactgcatga
    79261 tcggcacggc cgtgccagag gtgcaccgta cccgtcctgc ggcagcgtag ccgcatcaac
    79321 gcttaagtgc cataagattg catctgcgtc gcagcgagcc gggctaacct ctagaacttt
    79381 aacaaattcg ttacgcacgt ccgcctttat tgaacggcca acagtatcac ctctggccgc
    79441 gcggacgtgc tgcatcatgc cccccactgc caatcagacc tccggtctat tcggcatcgc
    79501 ctttctgcag gcggtgaggc gactgttgcc acagctcctg ctggcgatga tcgcggcggg
    79561 gctcgcgacg ttcctggcgc agtcctggat aaccccgcgc tacgtcgcgg aggcgcggct
    79621 cctgcccgac gagaactccg ctgcgcacag cgatcttgaa gctcatctca aggcgctgaa
    79681 ggacccgatc cggcttttcg aagtcgcgac cgagctcggg ctcaagagag tgccggaatt
    79741 cagtgggaag aagcgcggaa gcataccgat cctgtcgcag ttctggtccg acagatccgg
    79801 gcctctacag cggcttcagg accaggatga gcgcgtgctc gctgccgtct acgagaaata
    79861 ttccgtgagc atggccggtg acggcacgat ccgggtgagc ttcacggcgt ccgatccaca
    79921 acttgctgcg cgtttcgtga accgcctggt cgaagtctac ctcgccggcc tgtggtcgcc
    79981 tcagccctct gcgtcggaat cgaaaccgac cagcattcac gatgtgttgt gggcggaggc
    80041 gccggaaaag ccggttttcc cgcgcaaggg tccgatggcc atcctagcca tggtcctcat
    80101 gttgctcctg ggcctcggcg gcatagcgct gcgcgaggcg atccacacga cgccgcggcg
    80161 gggcaggcaa cctgtggagg acctaaccgc ggcagaggag aacaacgccg ccgccctcgg
    80221 cttccgcaag ctgcgttcgc cgattgcagt ggcggatcat ctgcttgccc ttcccgctgc
    80281 cgatcgcggt tgccgtacga tggttgcagg cgaggagccc gatatcgatg ccacagccga
    80341 ggctttggcg ctggcggacg ctctcaccgc cggcgggcgc cgcgtggtgc tcgtcagatg
    80401 gagcaggacc ggaggcgagg tgtcgggagc gccggcaatg caggggactt tggggttcaa
    80461 tgatctcctc gaagggcgtg cctcgttcga agagatcatc tcccgcctgc cgggcagccc
    80521 cgcccatgcc atcgcggcgg gctcggcgtt gcgtgatgag aaggcggcgc tcgatcccaa
    80581 tctcctcagc ctcgtgctcg acacgctgga tgaggtctac gatcacatcg tcgtcctggc
    80641 cgagcatggc gaagcgcggg ttctgttcga ggtgctcgaa ggccggttcg acgcatgcat
    80701 ctcggtcggc gaggccggac ggcctgccgg gattctgacg gcgagtttcg accggttcct
    80761 cgggttcgag gtgagcgaca tccatgtgct gcgcgtggag cggccgcgac agaaagcgca
    80821 gccaatcaca ccgtcgcccg cactgcagac gcggcttgcg tgaaagtggt ctcaggcccg
    80881 gcgtccggct cgttccgggg ctccatcagc ttccccggcc tcgtcagggc gcgacatcca
    80941 gcggaccagg agggcagctg gcacgaccca ggccaagccg ccgacgacgt agaacacgac
    81001 ctcgacccac ttgctggaat tgacctgcag cacgatcgcg accgccatga cgaggagcgc
    81061 gtagatcgca atgaagatca tgagcaggat agcgccgata aacttgcggg tgcggatatt
    81121 caccttgatg ctttctccgt gggtctctgc gatcaccgcg ccgactgggc gcattgcttg
    81181 agctgagggc ccctactatc agaaatcggc cagtcatttt cgggaaagat cgacacatgc
    81241 ccgagccgca ggtagcgcac acagccgcgg tgatccgaga gcgcgagcct gacacccgcg
    81301 ccgtcagggc gtggctttat ttcatggccg cgctgatcgt cgccatggtc gtggtcggcg
    81361 gcgcgacgcg gctcaccgac tccggcctgt cgatcaccga atggcaacct ctgctcggcg
    81421 ccattccgcc cctcagcgag gccgattggc acgaggcctt cgccaagtac cagcagattc
    81481 cccagtatca gctcgtcaac aaggggatga gcctcgagga gttccagttc atttactggt
    81541 gggagtgggc acatcgcttt ctgggacgct tcatcggagt cgcgtttgcg attccgtttc
    81601 tctggttctg ggcggcgggg cggctgccga aaggcacgcc gatgaaactt gcgggtgttc
    81661 tggcgctcgg cgctgtgcag ggtttcgtcg gctggttcat ggtgaagtcg ggcttggtcg
    81721 accggatcga tgtcagcccg ttccggctcg cgctgcattt gatcgtcgca ttgatcatcc
    81781 tggcctgcat cgtctggctg gcgctcgatc tgagaaagga gagcggatcg gtgcggctgc
    81841 gcacggtgac gcggggacag aagcggctgg cggcgaccct ggtcggtctc gttctcctgc
    81901 agtcgctgct tggggccttc gttgccggcc ataatgcggg cctcacctac aacacgtggc
    81961 cgctgatgga cgggcgattc attcccgaag ggctcggcac gctgtcgccc tggtggctga
    82021 acattttcga gaacatcacg accatacagt tcaaccaccg gatggtggcc tatctgctcg
    82081 cgggcttggc gctcacccag ttcgtctgga tcttgaggac agccgatgac gaggcgctga
    82141 cgcgctcggc cggaatcctg gcgctcggga tcttcgttca ggccgggctt ggcatctgga
    82201 cgcttctcga agtcgtgccg ctctggcttg gccttgcaca tcaggcaggc gcggcgatcg
    82261 tgctgatcct ggctgtgcgg cacgcacatg ctgtgatgcg cgcgccggcc gttggtaagg
    82321 cggtctccgg cagctactcg gcgagcccga gcatcagctg aatgttctga acggccgcac
    82381 ctgacgcgcc cttgccgagg ttgtcgaggc gggcgaccag cacggcttgc ccgcgtgcct
    82441 cgttgccgaa cacgcgaagc tccagcttat ccgtgttgtt gagcgcctgc ggttcgaggc
    82501 gcccgctccg caccgagccg tcggaggagg gcaccaccgt caccagaccg gatcccgcaa
    82561 agtgggcgct gagcacggcc tcgatgtcgg ccggggtcgg cttgcccggc aacgagtcca
    82621 ggtgaagcgg tacgctcacc agcattccct gccgaaaatt tgcgacgctc ggcacgaaga
    82681 tcggccgccg ggagagcagc gagtaggctt gcatttccgg cagatgcttg tgctcgaggc
    82741 cgaggccgta gagctcgaac gccggggcgc ggccgctttc gtaggcctcg atcatggtct
    82801 tgccgccacc cgagtatcca gagacggcgt ttatggtgag ggggtagtcc tgtggcagca
    82861 gccccgcggc gacgagcggc cgaatgagcg cgatgacccc tgtgggatag cagccgggat
    82921 tggcgacccg gcgcgcgctg cggatggcgt cccgctggcc ggcatccatc tcgggaaatc
    82981 cataaaccca gcctggcgcg acacggtgcg cggtcgaggc atcgacgatc cgcgggccgc
    83041 cgtcgggcag cgtatcggcc atggcgaccg tctcgcgggc cgcgtcatcg ggcagacaca
    83101 gcacgacgag gtccacttcc ctgagcaggg cacgcttggc ttcgaggtct ttgcgcctct
    83161 cgggcgcgat gctgcggagc tcgatggcgg gtatggcgcg gagccgctcg gcgatgccga
    83221 ggccggtggt gccggcctca ccgtcgatga acacggttgg cttgcggcta tggctgctgc
    83281 tggtctgcgc tggcgaagct gatgacatcg cgtgcacctc ctgtggggca tgcaatcctg
    83341 ggacaggaca caaaaaagcc gcgcggaacg gcgcggctca cgacaccgga cgaaagtcgg
    83401 ccgtcatcgc aaccgcggga ggagctcgcg gcggcgtcgg cgcaggttct cgaaatggcc
    83461 actcgtcatg cgcgacatat gtggcagggg gaggggaggt gtcaacggcc aactggccgg
    83521 cgctgatcgt gtcgcaatgc cagcttgggc gttggcggca agtcgaatcc cgggcgcagg
    83581 gcccgggatg acacggttgc aatgctgccg aggcggatca gcgcttcgag aactggaagc
    83641 tgcggcgcgc ctttgcccgg ccgtacttct tgcgctcgac ggtgcggctg tcgcgggtga
    83701 ggaagccgcc cttcttcagc acgctgcgca gctctggctc atagtaggtc agcgccttcg
    83761 agatgccatg gcggacggca ccagcctggc ccgacaaccc gccgccggag acggtggcga
    83821 cgatgtcgta ctcgtcggcg cggttggcgg cgttcagcgg ctgcttcagc agcatctgca
    83881 gcaccggacg ggcgaagtac tgcgcaaagt ccttgtcgtt gacggtgatc ttgccgtggc
    83941 caggtttcag ccaaacgcgc gcgatcgcgt ccttccgctt gccggtggcg taagcgcgac
    84001 catactgatc gagcttctgc acgtggacgg gagcggcgcc ggcctcgggc gtactgcccg
    84061 tgagtgttcc gagatcttcc agcgacttgg tggtatcggt ggccatggga atcaggccct
    84121 cacattcttt ctgttgagct tggcgacatc gagcacttcg gggttctggg ccacatgcgg
    84181 atgctcgggg cccttgtaga ccctgagatt acgcatgagc ttgcgctgca gcggcccgcg
    84241 ggcaagcatg cggccaacgg caagctcgat cacgcgctcg ggaaacttgc cttccatgat
    84301 catgcgcggc gtgcgggcct tgatgccgcc cggatagccg gtgtgccggt agtagatctt
    84361 gtcgttgtac ttgttgccgg tgaacacggc cttctcggcg ttgacgacga tcacgttgtc
    84421 gcccatgtcg acgtgcggcg tgtaggtcgg cttgtgcttg cctttgaggc gggtggcaat
    84481 gagcgccgcg agccggccca cgacgaggcc ctcagcgtcg atcaaaaccc attttttctc
    84541 cacctcgccg ggcttggcga cgtaggtttt catgtcggtg gctccaggga aaccggaatc
    84601 gtaaggcgtt cagctatcgc tggcacgcgt tcgacgcgtt gataggtgag cgcagaaccg
    84661 gcgtcaacat ctttctctgg tcgatcagaa aaaatcaatg ttttcagtgc tgtagcataa
    84721 gcggtaatat attaccgtaa atctcttctg tgcccagccc gacgacttca cgacctgacg
    84781 atttcacgag ttcacccctc acacgccctt ctccggcggc agtgcatacg tcgcggtcgc
    84841 atgagccacc atctccggtg cgtcggccga atagatctcg gcttctgcga cggccaggcg
    84901 ccggcccagt ttcagcaggc gcgccttggc gatcaagtcg ccgggctgcg gccgggtgag
    84961 gaagttcatt gtcatgctgg tcgtaaccgc ctgcagtgcc tgcgccccga gctcgccaag
    85021 tatcatcgcg tagacggaga aatccgccag cttgaacatg gcggggcccg acaccgtgcc
    85081 gcccggccgg atcgagcgcg gatcatgcgc catgcgaacg tgagcggtca tgggacccag
    85141 gaactccagg ctcacgcgtc cgttcccttc gtgcacctga ggaaaatgtt catccaccag
    85201 cgccatcacc tgttccttcg tcaggcgctc gcgggcagcc gcttcgtttg ccattcctaa
    85261 tatcgtctcg attctgctat caattcgccg aagccgacta ttagcctccg ctgaggttca
    85321 ggggaatggt gggagggatg ctcatgtcac cccgtttggt ccagatgctt cgttcagtgt
    85381 caattctcgc gtttgcgtta gccttcctgc tgagcccggc cgcggcagag gaatacgagt
    85441 tcaagcagat caagctcacc gagaagcagg tccagagctt cattaaggcg caggccgagc
    85501 tcaacaagat ttcggataaa ctgcagagcg attcggagga gccggatgag aaggtccagg
    85561 cggagctcga ggccatagcc aagaagaatg gtttcgccaa cttcgatgag ctcgaccagg
    85621 tagccgccaa cgtctcaatg gtgatcgccg gcatcgatcc ggagaccggc gagtacacgg
    85681 acccggtcga gtcgatcaag aaggagatcg aggagatcaa ggccgacaag tcgatccccg
    85741 agaaggagaa gcagcagatg ctcaaggaga tggaggaggc actggaaacg actccggccc
    85801 tggagcatcc tgagaacatc gagatcgtaa agaagtaccg cgccgagata gacaaggcgc
    85861 tgcagtagat cgaattcgga atgcaggagc gcgttgaggc gagcaagacc gcagccattc
    85921 tgctcgagga gcggaaaggg gccgttgcga tcctgactct caatcggccg caagcccgca
    85981 acagcctgtc gctggagctg ttgcgggctc tcggtgccgc catcgaacgg atctcggccg
    86041 acgctgaggt gcgtgccgtc gttctcgcgg cgaacgggcc ggcgttctct gcgggtcatg
    86101 atctgaagga gcttacggct catcgcgccg acaccgacgg cggattggcg ttctaccgaa
    86161 cggccatgga cacctgctcc ggggtcatgc aggcgatcct caggtcgccg aagcccttca
    86221 ttgcggccgt ccagggcacc gcgacggccg cgggatgcca gctcgtcgcc acgtgcgacc
    86281 tggctgtggc cgcagacgag gtccagttcg cgacaccagg cgtcaacatc ggccttttct
    86341 gctcgacgcc gatggtggct ttgtcgcgca acgtgccgcg gaagcgggcg atggagatgc
    86401 tgctgctcgg cgagatgctg tcggcggagg ccgcggcaga gtacgggctc gtcaaccgcg
    86461 tcgtgccgcg gggccgcgtg atggacgagg cgctcgccat ggcaggtgtg atcgcgtcga
    86521 agtcctccct caccgtcgcg atcggcaagg aggccttcta tcggcagatc gagctgccaa
    86581 cggccgaggc ttacgcctat gctgcggacg tgatggtcaa gaacatgatg gcgcgcgacg
    86641 cggccgaggg gatctgcgct ttcatcgaga agcggccccc agagtggaaa gggtgctgaa
    86701 ttcgtgcagc tccgattgag cctcgtgacg ctcggcgtcg ctgatgtcgc ccgtgcgcgg
    86761 cgcttttacg aggcgatggg cctcaccgca tccagcgtga gcgaggactc agtcaccttc
    86821 ttccagctcg gaaacgtcgt cctggctctt ttcggacgcg atgcgctcgc agccgatgcg
    86881 ggtgtttctc ccgacgggtc cggctttgcc ggcgtcgcac tcgcttggaa cgtcgccagc
    86941 gaaggcgagg tggatgaagc gctgtcccag gcggtcgcca gtggcggccg gctcgtgaag
    87001 ccgggacaga aggttttctg gggcggctat tgcggctatt ttgccgatcc ggacggacac
    87061 ctttgggaag tcgcctacaa tcctttcttt ccgctcgatg cggcagggcg tgtttctctg
    87121 ccggatgacg ccgatttgcc ttcgagaaca gcctgatggc ccacgacagc tattccgacg
    87181 acctcatcgc aaccattctg cgcgagaaca gctcgatcgc catggtgggc gccagcgcca
    87241 ataccagccg gccgagctac ttcgccatga agtacatgct gggtaagggc taccgcgtgg
    87301 ttccggtcaa cgtcgggctc gccggccagc agatcctggg ccagacggtg tatgcgaccc
    87361 tcgcggatgt gcccccgccg atccggatcg tcgatatctt ccgcaattcg gaggcggcgc
    87421 tcgagatcac gcgggaagcc atcgcgcaga aggatcgcct cggtgttcgc gtggtctgga
    87481 tgcagctcgg cgtgcgaaat gaccaggctg cggcggaggc ggaggctgcc gggctgcagg
    87541 tcatcatgaa ccggtgcccc aagatcgagt acgggcggct ttccggcgaa atcggctggg
    87601 cgggagtcac caccgggcgc atctccagcg tgcggccgag actgtcgccg cacggcgtgc
    87661 agaaccatct catcggcgac aaggcctgag acggacgcgc aactcacatc cagtcaggtt
    87721 cttcctcttc gcgctccagg atttcgcaca gctcggcaac cttgccgctc ggctggcggc
    87781 ggagctcgcg gccgatgaca tcgtcggcat tcagccaggc gaatgcctcc tccagctcct
    87841 cggggctggc cccggtgccg atgatctcga cgatcagggt ctcatcgaca ggtccgagga
    87901 cgcgcacgac gtcctctcgc gtcatggtca tcgtggcatt ctcctgatcc atcggaatag
    87961 gccaatgggc ctgtatgtcc gagacaacca tcgagaatga ggttggttcg cgcgcccggc
    88021 tgcaagagcc cggcgtgtcc gctgcttccc cggcatcggg gccgcgttga gccgacgtca
    88081 gatccggttg ggatagaaaa ggtcgaccag gaacatcggg atggccggca cataggtgca
    88141 caggaacagc acgaacagca gcactaggac gaaccagatg ttgaccttgg atgtctccca
    88201 gacgtcggat ttggcaatcg agcaggccgt gatcaggaca ctcgccaccg gcggggtctg
    88261 ctgaccgatg gccagattaa gagtcaccac caagccaaag tggaccgggt cgatgccgac
    88321 cgtgttcatg agcggcacga tgatcggcac gaccaggatg atcgccgcag ccgagtgcag
    88381 gaagaagccc aatatcagga agaaaatatt gagcatgaac aggatcacgt actgattgct
    88441 cgaaactgac gcgatccact gagcgaggtc ctgcggcacg cgggcgaccg tcaggaagtc
    88501 gcccatcaaa atcgaggctg cgaccagcag catgacgacc gccgtctgca cggcaccgtc
    88561 gagcatggcg gacttgagca gcttgaactc gagctccccg taccagagcg cgacgaggat
    88621 tgcggccaga acagcaaggc ctgcggcctc agtcgcggtg gcgaggcccg agaagatgcc
    88681 accgaggatg atgatgggca gcaacaaggt cgggatggcg tcgatgaacg tcttcttcag
    88741 gttcgcgaga ccaaacgctt cctcgacagg gaaatcgtac tttcgggcga agacatagca
    88801 gacgaccatc agcaacaggc cgccaatcac gccgggtatg aaaccagcaa taaagagctg
    88861 ctcgatcgag gtgcccgccg aaattgcgta caggatcatc gggatcgagg gcggaatgat
    88921 gatggcgagc gatgccgatg acgaagtcac cgcagcggcg aattccttgg aatagccctt
    88981 gcgcttcatt tccgggatca tgaccgatcc cagggcggcc acgtccgcga cggcgctgcc
    89041 ggagatctcg gcgaagatca gcgaaacgcc gacgttcacc atcgcgagcc cacccctgac
    89101 gaagccgatc agcgaagaaa cgaacgaaat cagccgccga gaaatcccac ccgcgttcat
    89161 gatggcgccc gccagcacga acatcggaat ggcaatgagt gagaagctcg ttgccccgcg
    89221 gtacatctcg atgccgaccg agatgaggtt atcggtgcca ttcgtcgcag cgatgccgat
    89281 gacggcagag atggctaggg caatggcaat cggtacgttg atgaggatca tcgccagcat
    89341 ggcgatgaac atgatgaaca gcagcatcgg attacgtgct ctcgggaacg tattcggggt
    89401 gttctgacga gcggccggat atgacgttcc gccagtaatc gggcagactc acgagctcgc
    89461 agatgatgaa aagtgctcca ccgatgggga tgaccgactg cgttacggca ataggcatcc
    89521 aggtgaggct tacgagggtg tcaccgccga cgaccgagat cacctcgagg ccagcccatg
    89581 tgagcagcag aaaaaagccg atgacgagaa cctccccgat cacgaccgcg atttttcgcg
    89641 ccgtgagcgg caaccgcaaa agcaccccat cgaacccgat atgcgcccgc ttcaatgcgg
    89701 cgtatgccgc agcgtaatag gtgacccatg cgagcatcac ggaggaaact tcgtcatacc
    89761 agacgaatga agccccgagc ttccgggcga agacagcaag aatgacgatc accgtgagca
    89821 tgaccatcag gaaaatgacg gtccattgca gcagtgtatc gagagttcgg cgcaggctgt
    89881 tcatcaggga ctgctttaat ctgatcggga gtggtggaat ggggagaggc gcgtgcgcgt
    89941 cctcaactac ttttccatag cagacggccc ctgccgcagt cctcagcagc aggggcccga
    90001 gctggccgaa cttattgcgc cagggcttgg atttccttga ccatctcggc gccgttcttg
    90061 accgacttgc cgaactcctc gtacaccgcg ccggactggg cgatgaaggc gtccttgtcg
    90121 acctcgtttg ccttcatctc cgaggcactg gtcatcttct cgagcagctc gttgtcgagc
    90181 ttggccgcaa gctcgcggac gtactcttcg gtttccttcg ccgcctcgga gatgctcttc
    90241 tgcacatcct caggcagctt ggcgaagtgg gtcggcgagg cgacgagcca ggccggcgtg
    90301 aagacgtggc cggagagcga gatgtatttc tgcacctcgt agaacttgcc gccccagatc
    90361 tgcgggtagg ggttctcctg gccgtccatt acgcccgtct tcaaagccgt gaacacctcg
    90421 ctgtaggcca ttggcgtcgg gttggcgccg taggccttga acatcttgat gcgccactcg
    90481 ccagcgggaa cacgcagctt gatgcccttg aggtcctcgg gcttgacgat gggacgtaca
    90541 ttgttggtga tgtgccggaa gccgttctcc cactgaccga gaacgagcac gcccttggcc
    90601 ttggcggcag ggatgaaata attgtcgccg agcttttcca tcaccagctt catgtgctcg
    90661 cggcttttga tgatgtaggg cagctcgaac acgccgaact cgtcggcgat ggacgacatg
    90721 atcgtcgacg ggatccagag ctgaagctga tttagcttca atttctggta catgtcccgg
    90781 tcgctgccga gctgactcga gggatagacc gtgatcttcg ccttgtcgcc gagcttctca
    90841 ttggcgcgcc tggcgaattc gtcggcggac tgcgcgatga gcgaaccggg ctctgccacg
    90901 tgaccgagct tcagctcgat ctgcgcgacc gcgccgctgg cggacagcag gacggctgcg
    90961 agcgcagcta gggatgttct gaaaagcatg cttggggtcc tcctccgatc cttgttgcac
    91021 cggcccgttg cagggctctg ctaggcatac tagcgagcat gagataagcc gtcacgaaaa
    91081 tccatgattt gacgactttc gcgtccacgt cccggctgcg aggcgcgcca gaattccagc
    91141 cacggtcgag aaattcgttc ggaacaactg cggcgggtat cgggggattt tcctatcacg
    91201 acgctaccgg attatacgcc ccggctcgtg ctgcgcagat agtccatcgc ggcgagcacg
    91261 ccccctgtct tgtgcggcac gcccgcaagc ccgaggccca tttcgactcc ggcgagtgcg
    91321 ccgatgatgg tcaggtcgtt ggtgtcgccg agatggccga ttcggaacac cttgcccgcg
    91381 agcttcgaca gccctgtgcc gagcgacatg tcgaaatgct cgagcacgat ctgccggaaa
    91441 cggtcggcat catgcccttc gggcatcagc acggcagtga gtgaggaaga gtagcaggcc
    91501 gggtcgcggc agaggatctc cagcccccac gcccgaacgg cccgccgtgt cgcctccgca
    91561 tgccgatcgt ggcgcgcgaa aacgcgttcg agaccttcct cgtgcagcat gtcgatggct
    91621 tctgcgaggc cgtagagcag attggtgccc ggcgtgtacg ggaagaagcc gttcttgttg
    91681 tgcggcgcca tctcggccca cgaccagaac agcttcggca gccgcgacga ttcggacgcc
    91741 ttcagcgcct tttccgaaac cgcggtgaac gacagccccg gcggcagcat caggcccttc
    91801 tgggagccgg acaccgtgac gtcgaccccc cactcgtcgt ggcggtagtc gatcgatgcc
    91861 agtgaggaaa tggtgtcgac catcagcagc gcaggatggt tcgcggcatc gatcgccttg
    91921 cgaacctgag cgatgttgga cgtgacgccc gtcgaggtct cgttgtgcac aacgcacacc
    91981 gctttgatgg cgtgcgcctt gtcctcgcgc agccgcgcct caatggcggc ggcatcgacg
    92041 ccggagcgcc agtcggacgg gatgaattcc ggcttgaggc cgagcttttc cgccatcttc
    92101 ttccagagcg tggcgaagtg gcccgtctcg aacatcagaa cctgatcacc cggagacagg
    92161 gtgttggcga gcgacgcctc ccacgcgccc gtgccggacg ccggatagat gaacaccggc
    92221 ccggtcgtct tgaagatgct cttgatgcct tccagcaccc gcttgccgag cgcctgaaac
    92281 tccgggccgc gatggtcgat ggtcggcagc gagattgccc gcaggatacg atcgggtacg
    92341 ggtgtcggtc cggggatctg cagaaaatgg cggccggcgc gacgcggcac attcgacatc
    92401 agacgatttc tcccgcccgc caatggggcg ctatgggttg ttgcgctttc agatgcttgt
    92461 atgcgatcga cggacccgag ttcaagccat atgggcccgt actgggaaaa cctgatgaac
    92521 gtgcacgtac ccgccaatcg cgccctcgcc gagcggctcc gccgcgagat ccagggagag
    92581 gtgctgttcg accgtttcag ccgcggacgg tacgcgacgg acgcctcgat ctatcagatc
    92641 gagccggtcg gcgtcgttgt cccgaaaacg gacgaggacg tccgggccgc cttggagatc
    92701 gcgcgggaaa gtggcgtgac ggtgcttccg cgcggcggcg gcacatcgca atcgggccag
    92761 acggtcgggc gatcgctggt catcgactac tcgaagtatc ttaaccggct cgtcgagctc
    92821 gatccggaag cgggcacgtg cgtcgtggag cccggcatcg tgctcgacga gctgaaccgg
    92881 caactcaagg cgaccggctg gtggttcccg gtcgacgtgt ccaccgcaag ccgcgccacg
    92941 ctcggtggca tgaccgggaa caactcctgc ggcacccgct cgctgcgcta cggcatcatg
    93001 cgggacaacg tgctcgccat cgacgcgctg ctccccgacg gtacgccgac gaggtttgca
    93061 gagatcgatc cttcggacct cggtggttgc gacgctgaag cgcttttccg gaggctgatt
    93121 gccctcgggc agcgggaagc cgcgcacatc gcgcaggcct ttcccgaggt ctcacgccgc
    93181 gttgggggct atctcatcga cgcgttggtg ccgggcaaca agccgctcaa cctggcgacg
    93241 cttctcgtgg gctctgaagg cacgctcgcc ctgtcgcggc gcatcgcggt gaagcttgcc
    93301 cgccagccga agaacaaggc gcttggcatc tgccatttcc ccaagttccg gcaggcgatg
    93361 gaggctgcgc agcacatcgt gaagctgggg ccggtggcgg tcgaggtcgt cgaccgtacg
    93421 ctgatcgagc tggcgcgcga catcgccatg ttccggccag tgatggacac ctacgtgcgc
    93481 ggcgagcccg atgcgctgct gctcaccgag ttcgccgagg acgaccaggc ggagaacctg
    93541 cgccgcctcg accgcctgga agagctgatg ggcgatctcg ggcatccgga cagcgtcgtg
    93601 aaggtggccg acgcggccgg ccagaaggcg gtgtggggtg tgcgtgcctc cggcctcaac
    93661 atcatgatgt cgatgaagag cgaggggaaa cccgtctcct ttatcgagga ctgtgcggtt
    93721 ccgctcgaga accttgccga ttacaccgag cggctgaccg cggtgttcga gaagtatggc
    93781 acgcgcggca cctggtacgc gcacgcatcc gtcggctgcc tgcatgtgcg gcctgtgctc
    93841 aatctcaagc tcgagaagga tgccaacacc atgcgcgcca tcgccgagga ggcgttcgcg
    93901 ctggtcaagg aatacaaggg cgcgcattcg ggcgagcacg gcgacggcct cgtcaggtcc
    93961 gagttccacg agatgatgta cggtcgaaag accgtcgagt tgttcgagga ggtgaaggac
    94021 acattcgacc cgacggggct catgaatccc ggcaaaatcg tgcgatctcc acgcatgaac
    94081 gaccgcacgc tgttccgcta caagccgggc taccacgtac cggagtttga gactgtcctc
    94141 gactggtcgg cctatcccgg cgccgggaag ggtttccagg gcgccgtcga gatgtgcaac
    94201 aacaacggcg agtgccgaaa gctcgacggc agcgtgatgt gcccgagcta tcgcgtgacg
    94261 cgcaacgagc gcgatgtcac gcgcggccgc gcgaatacat tgcgccttgc gatctcgggt
    94321 cagctggggc cgggcgcgct cgccagcgac gagatgctgg aaaccgtcaa gctctgcgtc
    94381 tcctgcaaag cctgtcggcg cgagtgcccg accggggtcg acatggcgcg catgaagatc
    94441 gaggtgctcg cggcggcggc gaagcgccgg ggcgtgtcgt tccgtgatcg gctgatcgcc
    94501 tatctgccgc gctatgcgcc ctatgcggcg cggcttgcgc ctctgatgaa cgcgcgcaat
    94561 gtcatccccg gtgctgcgtg gctgacggag cggttgatcg gctttgctgc aaaacgcaag
    94621 ctgccccgct ggacgatgcg gccgttctct gcatctgcgc acaagcgggg tggggcgagg
    94681 ggtgaaagtc ggccccaggg gcagaccgtc ggcgaggtgg cgctgtttgc ggatacgttc
    94741 aacacttact tcgagcccga aaacctccac gccgccatcg aggtgctgga acggctcggc
    94801 tacagggtgc acgttctgga gcctgtcggt ggcgggagac ctgtctgctg cgggcgaaca
    94861 ttcctctccg ccggccttgc ggacggggcc cgcgccgagg tgaagaggct gctcgccgcg
    94921 gtgaagccct tcaccgaacg cggcgttccg atcgtcggcc tcgaaccgtc ctgcctgctg
    94981 tcgctgaggg atgaaatccc cgccctgttt ccgggagagg aggccgcgaa ggcagccgaa
    95041 tgcgcctggc tgttcgagga gttccttgcg cgcgagatcg atgccggacg ccttgctggc
    95101 ccaatcgcaa ggtcgtccgg acacctgctg ctccacggcc actgccacca gaaatcgttc
    95161 aacgcgatgc cggccgtcac caaggtactt
//
LOCUS       DASVDU010000267        52206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_254834, whole genome shotgun sequence.
ACCESSION   DASVDU010000267 DASVDU010000000
VERSION     DASVDU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 52206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52206
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254834"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2452)
                     /locus_tag="VF226_17730"
     CDS             complement(<1..2452)
                     /locus_tag="VF226_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7075881.1"
                     /translation="MTGRTDVGQRDVRRLNQGRALWLSTLAFAISFAVWTIFAIIGIR
                     IKAELGLNETLFGLLVGLPIAVGSLIRVPVAIWSEKYGSRVVYSLVMLGGALGAWLLT
                     FAGSYPTILLAGVAVGIAGGSFGVGVTYLSKWYSPEQQISTLNIFGLGNVGAAVTMIL
                     GPFVLAAYGWHGVARIWALALAVMAVAFWVMARDEPPIAARERTGEPPPTLAAMLAPL
                     RRVQVWRFSLYYFFVFGAFVALALWLPRYLIGAYGVNLQTAGVLGSAFAIPASVFQIY
                     GGRLSETYGARRVMYWTFGVSTAVCLILSYPPTEYIVRSARGPISFHFEIGLTAFTAL
                     LVVLGFFMSLGKAAIYKHIPVYYPENVSAVGGVVGMVGGLGGFVLPIAFGILVDLTGI
                     WTTVFMLLFAISALALIWMHVSIRAMERGVLAPELRKLPELPEMQEVHEPRHIGVLGP
                     HLLTDWRPENEEFWEDKGRAIAKRNLWLSIPALLLSFAVWMIWSVIVARLPAVGFPLS
                     TNQLFWLAALPGLSGAVLRIFYAFMVPVFGGRLWTTLTTWSLMIPAIGIGLAVQNPET
                     PYWVLLLLSLLCGLGGGNFASSMANISFFFPRSEKGHALALNAGLGNLGVSVVQFVVP
                     LVITVGIFGWLGGGPQFVQTESGVMPIWLQNAGYIWVPFIALAAFACWFGMNDIETAQ
                     SSFAEQSIIFQRKHNWIMCWLYTGTFGSFIGFSAGFPLLAQSQFPDVDALRIAFLGPL
                     IGALVRSGTGWLADRYGGARVTFWVFVLMSVGVLGVLYALSIGSFTLFLLSFLFLFFM
                     SGVGNASTFQMIPVIMRLE"
     gene            complement(2515..3375)
                     /locus_tag="VF226_17735"
     CDS             complement(2515..3375)
                     /locus_tag="VF226_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7075882.1"
                     /translation="MALRVNGVEITEERIREEMQYHPAPSPDDARREAVNALVVRTLL
                     LSEVRRLGISAPRPLTDATKKLVEAPEEALIRALVDREIEVAEPSEEDCRAYYLENPQ
                     RFKSPDLLQAAHILFSANPNDRESMAKAKARAEAALKQLRDDPARFGELAREHSDCSS
                     REHGGDLGQITRGSTIPEFETFLFALEPGQICPVPIRTRYGYHVVRLNQRLNGRQLPY
                     ESVKDRIKAYLREKTWREAVHNYIARLKATAIIEETDEPADREFSGCGPRRGCGAAPR
                     VPPNFGVEPT"
     gene            complement(3379..4143)
                     /gene="narI"
                     /locus_tag="VF226_17740"
     CDS             complement(3379..4143)
                     /gene="narI"
                     /locus_tag="VF226_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX7075883.1"
                     /translation="MRAQILQVNPRVKSSVEVIPMSWMDQFFFGFYPYLCMVAYFLGA
                     WARFDRSQFTWRSQSSQFLQKRYLFWGSYLFHIGIIGLFFGHFFGLLTPPWVYHALGL
                     STKAKQMLAIIVGAVLAIICFIGATMLIYRRISESRIYVTSKPTDLFIILFIYVQLII
                     GIAGIPVSYQNIDGSYMLLLAEWSRSILSFQPGSAALLENVPWIYKIHLIFGMTLFLI
                     TPFTRLVHIFSAPIWMLGRPGWQIVRRNQNLSKRGV"
     gene            complement(4094..4810)
                     /gene="narJ"
                     /locus_tag="VF226_17745"
     CDS             complement(4094..4810)
                     /gene="narJ"
                     /locus_tag="VF226_17745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEX7075884.1"
                     /translation="MLTFKVLSGLLLYPTSELNQARAEMLAILEREGLVNESLIEGLR
                     SLMDRRAVASPLDAEAEYVALFDCSRSLSLHLFEHIHGDSRERGAAMSDLIEQYRRRG
                     LEITADELPDYLPLFLEFLSMQPLEDARAMLAEVADILALLQARLSERSSQYAVVFRV
                     LCAIAKAHVDPAAVAAAVAAEQPVDLDKEWEEAPVRFDLPLSDQAAGECRIAAAMVER
                     MEREIAGADPPGKSTGEIER"
     gene            complement(4825..6348)
                     /gene="narH"
                     /locus_tag="VF226_17750"
     CDS             complement(4825..6348)
                     /gene="narH"
                     /locus_tag="VF226_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEX7075885.1"
                     /translation="MKIRAQIGKVLNLDKCIGCHTCSITCKQVWTTRQGVEYAWWNNV
                     ESKPGIGYPKDWENQRRWQGGWRYKPDGTIEPRIGGKWRILAKIFANPHVPQIDDFYE
                     PFDFEYSYIHHAPESEAAPTSRPVSMITGERMQKIRWGPNWEESLGTEFSKRSEDYNF
                     EGVEKEIYGQFENTFLTFLPRLCEHCLNPSCLAACPTGAIYKREEDGIVLIDQEKCRG
                     WTMCVTGCPYKKIYYNWHTGKSEKCIFCFPRIEAGLPTVCSETCVGRLRYLSVMLYDA
                     DRILEAASVADEKDLYQAQLDIFLDPYDPDVQEQARRDGVPESWIEAAQRSPVYKMAK
                     EWQVAFPLHPEYRTLPMVWYVPPLSPIQHAAEMGAIASLHGIPDVSSLRIPIRYLANL
                     MTAGDEAPIRRCLERMLAMRAYMRRKQIDGMLDLGVLERVGLTQHQVEEMYRYMAIAN
                     YEDRFVIPTSHKETALRNAYDQRGVCGFNFTQNCSEGNSSFSLFGRDTRRTRGERFE"
     gene            complement(6348..10142)
                     /locus_tag="VF226_17755"
     CDS             complement(6348..10142)
                     /locus_tag="VF226_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEX7075886.1"
                     /translation="MSQFIDRLLFFRKYVGQFADGHGVVTNENRAWEDNYRTRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLITWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     IYSSNRIKYPLIRARLLQLWREARATLPPVEAWASIVEYPERANHYKSVRGLGGLVRA
                     DWSEINELIAAANVYTVRTWGPDRIAGFSPIPAMSMVSYAAGTRYLSLIGGTCLSFYD
                     WYCDLPPSSPQTWGEQTDVPESADWFNAGFLLVWGSNISMTRSPDTHFYTEARYRGQK
                     SVVIAPDFIECAKFADLWLAPKQGTDAALALAMGHVILKEFYIEKTTPYFDDYVRRFS
                     DLPLLVRLEKRGDRYVPGALIRASDFADKLGQDIYPEWKMVGIDESSSDVVVPQGSIG
                     FRWERSAKQAAQGEAPEPQTEPGRWNLEEKDGRDGRPIRLRLSLANSADEIVPVAFPY
                     FGSRAPGRFGLSDHDEIQVRNVPARKLSLNGGEELVATVFDLMVANYGVDRGLGGDIA
                     SSYDDNAPYTPAWAEAITGLPRQKIIDTARGFAANAEATEGRSMVVLGAGLNHWYHMD
                     MTYRGIINMLVLCGCVGKSGGGWAHYVGQEKLRPLAGWFPIAFATDWSRPPRLMNGTS
                     FMYAHTDQWRYERLKVSEMLSPLADPEEFKGTFIDCNVRAERMGWLPSAPQLNENPLK
                     VCRDAREAGKDPVAYTVEALKSGQLSMACEDPDNPKNFPRNLFVWRANLLGASGKGHE
                     YFLKHLLGTRNNVLNEDIGTTGGLKPVEVTWRDPVPEGKLDLLVTLDFRMNTTCLFSD
                     IVLPTATWYEKNDLNTSDMHPFIHPLCSAVDPVWESRSDFAIFKGFAENFSRVAQGVL
                     GEVEDIVLTPLMHDSPSELAQPFSGKDWRHGGSDPVPGKTMPQMTVVKRDYTAIAEKF
                     KSLGPLLERNGNSLKGILWSTADEVALLRSLNSTQAHGVGEGQPRLETDVDMIESILT
                     LAPETNGHVAVKAWEALEKSTGREHTHLAVFRKDEHIRFRGLVAQPRKIITSPIWSGI
                     ESEQVSYNAGWTNVHELIPWRTLSGRQQLYQDHAWMRAFGESLATYKPPINTRSLDDV
                     MERYDNGNPQIVLNFLTPHQKWGIHSTYTENLIMLTLARGGPIVWISEKDAQKAGIED
                     NDWIEAYNLNGALVARTVVSQRIPEGAAFMYHAAERMANVPGSEITGNRGGIHNSVTR
                     VVMNPVHMIGGYAQLSWGFNYYGTVGSNRDEFIVLRKIRELDWRDRPLAEKRPLMAEE
                     LR"
     gene            complement(10362..11792)
                     /locus_tag="VF226_17760"
     CDS             complement(10362..11792)
                     /locus_tag="VF226_17760"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX7075887.1"
                     /translation="MIPIRSGASISRRTLLGAMAGAGGYALLPRPLHAEMPGRPISLS
                     AEPGAARFLPQGEPTRIWGYGGTVPGPEIRIRQGGEVFARLSNGIGEPTTIHWHGVRI
                     DNAMDGVAHLTQPPVEPGQSFDYRFKAPDAGTFWYHPHAHQVMQQDRGLYGLLIVEEP
                     KPVAVDRDVSLIIDDWRLNADGSIETQSLAHLFDAAHAGRIGNLLTANGQVAPTLPAR
                     SGERLRVRLCSACNSRILELQFENARAQVIAVDGQPISPELMDGGASFVLPPSGRADV
                     VLDLTGEPGSQVSVREVTREPISIARFVLADDVRARERPLDTPVTLPRNPVPVPNLSQ
                     DPLVFPMLMEGGAMSSMAGAEYEGKYFSLRDLAQKHRMVWAFNGKAGNHISPLFTVER
                     GRPVVIKMTNATAWPHAMHIHGHHMLTISRTRGTLKPWFRDTVLMQPREEMSVAFLAD
                     NPGKWMLHCHMLEHQMAGMSAYFEVI"
     gene            11989..12804
                     /locus_tag="VF226_17765"
     CDS             11989..12804
                     /locus_tag="VF226_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7075888.1"
                     /translation="MPGKRGKIRIGVGGWTFEPWRGSFYPPGLPHKRELEYASRKLTS
                     IEINGTYYGSQKPEIFARWYEETPDDFVFAVKGPRFATNRRVLAEAGPSIERFFASGV
                     MELKEKLGPINWQFMPTKKFDPEDFAAFLKLLPTRIDGRAIQHAVEVRHESFCTPDFI
                     ALLRAHGVAAVIAGDSKYPRIADLTAPFVYARIMGTSEAVETGYKPAELDQWSKRARA
                     WAEGWVPPGLHTVGPAKGDRRPRDVFLYVISGAKVRNPAAALALIERIGKAGS"
     gene            complement(13998..15152)
                     /locus_tag="VF226_17770"
     CDS             complement(13998..15152)
                     /locus_tag="VF226_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX7075889.1"
                     /translation="MSRDLRPQTILAQAGGAVDAETGAIVPPVHITTTYLRDPDNQYR
                     RGYSYGRPDNPTVRQAEAVLTALEGAASSLLFSSGMAAATSLFLALPRPAHVIAPRVM
                     YWGLRKWLIEDAPNYGLSVSVVDSSDLDAIAAAVRPGETRLVWIETPANPLWTITDIR
                     GAAEIAHRAGAWVAVDSTVATPILTRPLAEGADVVMHSATKYLNGHSDTIAGVLSFAA
                     SETLFERSVAVRSRHGAILGPMEAALLLRGLRTLHLRVNAQCAAAMTIAQHFARHPQI
                     ADVLYPGLETHPGHAIAARQMRGGFGGMLSVRVKGGEQAAIAAAARVTVWKRATSLGG
                     VESLIEHRASVEGEGSPCPPDLLRLSVGIEAVEDLISDLEQALTANRGLQ"
     gene            15254..16081
                     /locus_tag="VF226_17775"
     CDS             15254..16081
                     /locus_tag="VF226_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075890.1"
                     /translation="MADRTVLITGCSSGIGLASARMMKARGWRVLATARKPEDLKRLE
                     ADLGLEALHLELADPNAVAACAQEALNRTGGRLYAVFNNAGYSQPGAVEDITAEALRR
                     QLEVNVIGPHDLTRRLIPAMRANGEGRIVTCSSVLGLVSAPWRGAYCASKFALEALTD
                     ALRLELQGSGIQVSLIEPGPIRTRFVENARAAAVANVDIEKSVHRERYHRMLAALERG
                     GRQAFKLEPESVARRLVHALESPRPKARYYVTTPTYAASILKRILPTKLLDRVAVRA"
     gene            complement(16088..19270)
                     /locus_tag="VF226_17780"
     CDS             complement(16088..19270)
                     /locus_tag="VF226_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEX7075891.1"
                     /translation="MRLSHFFIARPIFAAVISLFITLLGAIAYFGLAVTQFPEIIPPT
                     ITVTTNYPGASAQTVADTVASVIEEEVNGVDNMLYMYSNSTSDGAMSLTVTFDIGTDI
                     DQAQVQVQNRVSAAEPRLPEEVRRNGILTRKNNPDQLLAVHLISPDSTYDQVYISNYA
                     LLNVQDVLARIPGVGSVLLYGSREYAMRIWLEPERIASLGLSAEDVLRALRAQNVQVA
                     GGAIGEPPISGERAFQMPLQVKGRLREPDEFEKIIVKSGDDGRIVRLSDVARVELGAL
                     SYNSFGYMNHYPATVLFMTQAPGTDALKAANEIKRTMEELSQSFPKGLEYRIVYNPTE
                     FIEVSIKELYKSIFEAVGLVVLVMLLFLQTWRASLIPVLAIPVSLIGTFAVMQAFGFS
                     INMLTLFGLVLAVGIVVDDAIVVVENVERRLREGMPPVEAARLSMNEVGTALIAIAIV
                     LSAVFIPTAFVGGITGQFYRQFAVTIASATIISAFVSLTLSPALAAIMFRRHETAEGT
                     RSRLGRIVSMPIRALFRGFNRGFDALSRGYGRLVRRLAGLAASVLVLYAALIITTLWM
                     IQRTPTGFIPPLDRGFAIISMQLPPGASLERTNQVLQRANAIALDTPGVASTNVAVGR
                     SGATFTTSSSVGAIFVVLDTFENRSRQGLTVERVADELRRRLNDEIQEAQSIVIVPPP
                     VRGIGSSGGFAMRLQDRAGIGSVELANVAQKLIRAANERPEIVNTFTTFNTLTPQVFV
                     DVDRTKAQMLGVPIENIFEALRIYMGSVYVNDFNMFGRPFRVMAQAEGAFRLDRENVG
                     RIRVSNQDGQMVPLNSVVTFREMSGPDRVPRYNLFPTVEITGTGAPGISSGQALSVMH
                     QVAAEVLPEGVTYEWTDLSYQEVRAGHTGYYIFLLSVVFVFLALAAQYESWSLPLAIM
                     LIVPMCLLSALIGVWLHGQDLNILTQIAFIVLIGLAAKNAILIVEFARQIEEQGKDSV
                     TAAVEAGQLRLRPILMTSLAFTVGVLPLYFASGAGAEMRIALGTAVFWGMIGVTLFGL
                     MFTPVFYVVIRKLTAHRRPAANVNAAQPAE"
     gene            complement(19296..20327)
                     /locus_tag="VF226_17785"
     CDS             complement(19296..20327)
                     /locus_tag="VF226_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7075892.1"
                     /translation="MVREVTDWDEYTGRIEAVETVEVRARVSGYLEKVHFTDGQLVSE
                     GDLLYTIDPRPFERVLETARAELHEARVKAENAAKDVERGRPLVEKGWLSQRQFDERD
                     NLLREAQAAVAVAEARVKAAELDLSFTRITAPITGRTSRSELTAGNYVAVGGSENSPV
                     LTTIVRQDPVYVYFDIGENKALKYLRLHQVGQGTAVRVGLPDETGYPHQARLDFTDNR
                     LDTGTGTLRMRAVLDNKSGLFRPGMFARIQIAGSGSYSAIMIPDEAIGTDQVSKFVYV
                     VEDDGTAIRKWIEPGPLIDGLRVVRSGLSADEWTIVKGIQRVRHQEKVAVRKEPLQVS
                     AAPSKPVDQ"
     gene            complement(20779..21324)
                     /locus_tag="VF226_17790"
     CDS             complement(20779..21324)
                     /locus_tag="VF226_17790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7075893.1"
                     /translation="MPAAALMPLPERNFMKPEVTLKAVIDLSSQRMTVMTGDDRVLHQ
                     WPISSGRRGYHTPNGIYYPQWRSRMWRSRQYDGAPMPYSVFFHRGYAVHGTYATGLLG
                     RPASHGCIRLSTKNAATFYKLVEEHGMQSTQIIVKGQTRSAPARVARGRSSTPRRRIV
                     RQRVPRGYANYEYGTPSWWTW"
     gene            21968..23293
                     /locus_tag="VF226_17795"
     CDS             21968..23293
                     /locus_tag="VF226_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7075894.1"
                     /translation="MTAEARKRNISLGAAVADVEARYIAANPRSKARFEAAARTMPGG
                     NTRTILHYDPFPLGIVKGEGARLTDLDGHVYVDFLGEYTAGLYGHSEPAIAEAIREAL
                     SGGLVLGGPNAYEARLAELMCARFPAVDLVRFCNSGTEANLMCLSLARAATGRDAVMV
                     FNGAYHGGFLSFTKGHSRVNAPFDYVMGEYNDPEGVRRLIGEHASRLAAVIIEPMMGA
                     GGCIAADPEFLSMLREETRRHGIVFILDEVMTSRLAPGGLHGALGLTPDLVSFGKYLG
                     GGVTFGAFGGSRELMGLLDPTRPDGFMHSGTYNNNVLTMAAGVAGLEKVFTPERAEAL
                     NASGDRLRERLSAAIAKHDAPLQVLGRGSMLCIHPQRAPIRRPADHAAASADARKLLH
                     LDLKLRGLYTAPRGFMSLSLPLTEADHDALVAAFEGWLEDYAGALAQLD"
     gene            complement(23321..24256)
                     /locus_tag="VF226_17800"
     CDS             complement(23321..24256)
                     /locus_tag="VF226_17800"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7075895.1"
                     /translation="MVGDGRPGPGGSPASNVAAANLAQTNNTAGIIAMLAAMASFACG
                     DSLMKLSSTEMPTGQLVFLRATGMVGAAFTYAAFSGALRNAWKLLSRPMLLRAIGDVG
                     GALAFQSALARMAFADLVAIIQLNPLTVTAASAVFLNERVGWRRWTAAVVGLAGVMLI
                     IRPGTSAFTWWSILAIVSVLFATLRDVATKSIDRAVPAALIMLYSSGVVAIGSLFLVF
                     FEDWVLPGPGLLLQVAGASAFSFTGHICIISAVRKGELSAVAPFRYSILIWALILGLL
                     IWGDLPDTLTLLGMAIVTCAGIYTFRREMIVRRKG"
     gene            complement(24308..25405)
                     /gene="ruvB"
                     /locus_tag="VF226_17805"
     CDS             complement(24308..25405)
                     /gene="ruvB"
                     /locus_tag="VF226_17805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7075896.1"
                     /translation="MTDRLVTGAKRETDAFEATIRPQSLDEFIGQEQARANLKVFIQA
                     ARERGDALDHVLFAGPPGLGKTTLAQIMAKELGVGFRATSGPVISKAGDLAALLTNLE
                     ERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPAARSVRIDLSKFTLVGATTR
                     TGLLTTPLRDRFGIPIRLNFYSVDELELVVSRGARVLGAAISPEGAREIARRSRGTPR
                     IAGRLLRRVRDFAAVAGAKVIDADIADKALRALEVDTAGLDALDHRYLMCIATNYEGG
                     PVGIETIAAALSEPRDALEEIVEPYLLQQGFIGRTPRGRIVTLKAYRHLGLAGPARSS
                     SPQMGLFSSDVDDGEDIDSDPADRDRGARLR"
     gene            25595..25948
                     /locus_tag="VF226_17810"
     CDS             25595..25948
                     /locus_tag="VF226_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7075897.1"
                     /translation="MNWTRTRRAIALAVLTVAQPAGYLAGAAWAQEPALDPVHGRDVA
                     QRVCVGCHAIEPGVQQRQADVPSFPEIANRPGRTIQTIIGAMYYPHPEMPGVPLTTKE
                     TRDVAAYILTFRKAQ"
     gene            complement(25942..26376)
                     /locus_tag="VF226_17815"
     CDS             complement(25942..26376)
                     /locus_tag="VF226_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075898.1"
                     /translation="MTKAQRALWTFLFYTLVGPFIGALLLSVLMPLALVAGLLPEIAN
                     LEAAGEVAFVGWAAFFTYVWAAPASALAALGLLPLVLRNATFGWIAAAIAGVIAFAVT
                     AVLFALPVPEAIPYLAFLAGVVSVICRTILDRIGVLEPLPAH"
     gene            complement(26399..27028)
                     /gene="ruvA"
                     /locus_tag="VF226_17820"
     CDS             complement(26399..27028)
                     /gene="ruvA"
                     /locus_tag="VF226_17820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7075899.1"
                     /translation="MIGRLRGKVDAIGESYLIIDVNGVGYEVQASARTLRNLTLGDEV
                     TLSIETHVREDAIRLFGFTSEVERAWFRALQSVQGVGSKVALAILGTLSTQDLANAIA
                     LSNWAAVEQSPGVGKKLAQRVVAELKEKAPQLTVASVSVPAGLRVNGLAGPASQQDRS
                     TSAEAISALTNLGYSPQQAAAAIASAMGELGNTADIAQLIKRGLKELSR"
     gene            complement(27043..27570)
                     /gene="ruvC"
                     /locus_tag="VF226_17825"
     CDS             complement(27043..27570)
                     /gene="ruvC"
                     /locus_tag="VF226_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7075900.1"
                     /translation="MRGKSIRIIGIDPGLRRTGWGVIESDGVRLGYVASGQIASDRED
                     DLAYRLREIFEGLSSVIASFSPQEAAVEETFVNENARATLKLGQARGMALLAPAMKGL
                     RVAEYPPNLIKKSITGNGHGEKRQIQAMIGFLLPKAKFDNADEADALAIAICHAHHRA
                     ARQVVSARAGRQFGG"
     gene            complement(27652..28401)
                     /locus_tag="VF226_17830"
     CDS             complement(27652..28401)
                     /locus_tag="VF226_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7075901.1"
                     /translation="MAGHSQFKNIMHRKGRQDAVRAKLFAKLAREITVAAKSGLPDPA
                     MNPRLRLAVQAARAENMPKDNIERAIKKAQGNDSENFESVRYEGYAPGGVAIIVEALT
                     DNRNRTGGAVRAAFTKYGGNLGSTGSVAHMFDRIGEVVYAPGAASADAMLEAAIEAGA
                     EDVQSDENGHVVTCAFEDLGSVANALEEKFGQATSVKAIWRPNVTTQVGEDNAQTIMK
                     LISALEDDDDVQNVFSNFEVDEEVMARLTAA"
     gene            complement(28703..29533)
                     /locus_tag="VF226_17835"
     CDS             complement(28703..29533)
                     /locus_tag="VF226_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX7075902.1"
                     /translation="MRLLFLGDIVGRSGRKAVVDALPGLRRRYKLDFVVINGENSAGG
                     FGITEQILEELIGAGADVVTLGNHSFDQKEALVFIERHPRLLRPINLPPGTPGKGSGL
                     FRAANGAEVLVMNALGRVFMSQMDCPFRAIDHEVNACGLKRGADAIIVDFHAEATSEK
                     QSLAHFLDGRVSGVIGTHTHVPTSDYRVLPGGTAYMSDVGMCGDYNSVLGMDPTEPIN
                     RFLTRISRERFEPALGPATISGVAIEIDDASGLAVRVDVLRLGGVLAPAEPLSWVEED
                     "
     gene            complement(29533..30126)
                     /locus_tag="VF226_17840"
     CDS             complement(29533..30126)
                     /locus_tag="VF226_17840"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX7075903.1"
                     /translation="MVHPSAVSAAKQELRRTARRIRAEISKRERRWAAEQLAAYGIDF
                     VAPPAGAVVSGFFPVGDEIDPQPLMLRLAREGFQLALPVMDGKATPLRFRLWTPGDPL
                     EEKAWGIREPLPSAPEVISAVMLVPLLAFDAAGFRLGYGGGFYDRTLAAARSQGKITA
                     IGIAHDVQEVPAVPRLDFDQRLDWVLTPTGPRRCVRD"
     gene            complement(30202..30363)
                     /gene="ssrS"
                     /locus_tag="VF226_17845"
     ncRNA           complement(30202..30363)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF226_17845"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(30443..30697)
                     /locus_tag="VF226_17850"
     CDS             complement(30443..30697)
                     /locus_tag="VF226_17850"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7075904.1"
                     /translation="MGQVSVTLNGRTYRLRCEDGEEPRLFSLADHVKSKIEGLVAQFG
                     QIGDDRLMLMAALLVTDELFEAREQLGLMRSARTARAGVA"
     gene            complement(30740..31003)
                     /locus_tag="VF226_17855"
     CDS             complement(30740..31003)
                     /locus_tag="VF226_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075905.1"
                     /translation="MTQRDRQKKRRTAAPRASSRGARTSTPAASKSRASEVEQLLRER
                     DELRTRLDVAEARIKLLESQRLEVLNRIDWVIDSIHNVIERQN"
     gene            31257..32267
                     /gene="gap"
                     /locus_tag="VF226_17860"
     CDS             31257..32267
                     /gene="gap"
                     /locus_tag="VF226_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7075906.1"
                     /translation="MTIKIAISGFGRIGRNVLRAIAEQKRSEVEVVAINDLGSPEDNA
                     HLFKYDSVHGTYQGEVKVDGDLMDIGYGKPIKVLAIKDPAQLPWKDLGIDIVMECTGI
                     FTDKEKAKAHLEAGAKRVLVSAPSKGADRTVVYGVNHDKLTADDKVVSNASCTTNCLA
                     PVAKVLHELCGIKSGFMTTVHAYTNDQRILDQAHKDRRRARAAAMSMIPTSTGAAAAV
                     GLVLPELKGKLDGTAIRVPTPNVSVVDLKVIPERETTVEEINQAMKKAASQELKGVLA
                     INDKELVSIDFLHSPASSTFDVTQTQIVDGKLVRVLSWYDNEWGFSNRMVDTAVAMGK
                     LL"
     gene            32492..33526
                     /locus_tag="VF226_17865"
     CDS             32492..33526
                     /locus_tag="VF226_17865"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7075907.1"
                     /translation="MTERLEDIAQAMVTPGKGILAADESTGTIKKRFDSIGVENNETN
                     RRDYREMLFRATEAMRDYVSGVILFDETIRQKAKDGTPLVDLIKAAGSIPGIKVDAGA
                     KPLAGPTSRIEKVTEGLDGLRERLAEYYKLGARFAKWRAVITIADGCPTWNCVHANAH
                     ALARYAALCQEANIVPIVEPEVLMDGEHSSHDIDRCYEVTEWTLKTVFHELYVANVNL
                     EGMVLKPNMVIAGQKSPKQASPQEVAERTIKCLKACVPAAVPGIAFLSGGQSDEQATE
                     HLSLMNAMGNLPWKLTFSYGRALQAAALKAWGGKPENVAAGQRAFAHRAKMNGLASLG
                     RWERSLEQAA"
     gene            33957..34598
                     /locus_tag="VF226_17870"
     CDS             33957..34598
                     /locus_tag="VF226_17870"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7075908.1"
                     /translation="MEQEAGCQLFLVVEAGESAPDRLAAALEVVPIASVLIVPPAGQC
                     LAEASAKPLVEQAQAQGAAALVLDDVDLARKLRADGVHLQHSKDIESRYRAARQALGR
                     GAIIGVHAGKSRHDAMVVAEEGAEYVAFGLPEGVTDSASARERRLDLVAWWAEIFEVP
                     CVALDVAKPEDAAELARAGADFIGLTLRAGEPLSNIEQRMRAMAEAAQSALAE"
     gene            34612..35739
                     /locus_tag="VF226_17875"
     CDS             34612..35739
                     /locus_tag="VF226_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7075909.1"
                     /translation="MRTATKVLAGWLGLLIAFPAAFAETSSWSTETAPSVPKAKAQAK
                     PKPQSKPMARPQPKGPKPLQNNQAADPLSRFTPGKLESSLSRYLVPATGEDAAYIAFE
                     QGQYLTALNLAERAANEGDPQAHTLVGRIYAEGLGVPQNEAVAAQWYARGAELGDIES
                     AFALGLLFAEGKGVEKDRAAAARMFEMAAAKGHPYANYNLGVLFLNGDGKPENPTRGA
                     LHIRYAAEQGVAAAQYDLAVLYQKGYGVEPDAYQAALWMKRAADQGLVAAEYDYAVML
                     LRGFGFNADVPKAVDYLRSAAEKGFAGAQNRLAHVLADDSHGPPNLLEAAKWRILARD
                     SGLEDKELDELVANLSESDRLAAEKAAQEWRERAAIGVLTN"
     gene            35883..36683
                     /locus_tag="VF226_17880"
     CDS             35883..36683
                     /locus_tag="VF226_17880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7075910.1"
                     /translation="MPASALMNVMIAAARKAARSLARDFGEVEQLQVSLKGPANFVSA
                     ADRRAEEILFRELSRARPGYSFLMEESGEIEGADKSHRWIVDPLDGTTNFLHSIPLFA
                     ISIGLEREGQLVAGLIYNPISDEMYTAEKGKGAFLNDRRIRVAARQTLSDAVVATGIP
                     HRGRTGHLRFLREAEALMREVAGIRRTGSAALDLAWTAAGRFDGYWEHGLQAWDLAAG
                     VLIVREAGGLVTDTEGGSRIFENGDVLAGNPAISRALISVLNAAKRAA"
     gene            36782..37819
                     /locus_tag="VF226_17885"
     CDS             36782..37819
                     /locus_tag="VF226_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEX7075911.1"
                     /translation="MARKRQAEISAKAVHRTLSSPGGFLLNMIFFLTIVGFIAAIAMP
                     MLVTAFLNNPFLNSLIIFVLFIGILYSFRQVIRLYPEIRWVNAFRIADPGLAISHRPV
                     LLAPMATMLRDRTGTLSLSTVSMRSIMDSIGSRLDEARDTGRYMVGLLVFLGLLGTFW
                     GLLETITSVGTTISALDTSASESSMVFEELKTGLAAPLKGMGTAFSSSLLGLAGSLVL
                     GFLELQASHAQNRFYNELEEWLSGITELTPGGSGADQLNRQLYSAVFEMQRSITELTD
                     RLQAALPLDANGSGNDAVRDLAQGVNELVAQMRAEQNVVRQWVDEQAAQQNQVTGMLR
                     DLAMNFKRRGA"
     gene            37823..38851
                     /locus_tag="VF226_17890"
     CDS             37823..38851
                     /locus_tag="VF226_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEX7075912.1"
                     /translation="MARGRSRHGGQYGEFWPGYVDVLSTLLMVFTFLLSIFMLAQYFV
                     SQEASGKDAALRRLTRQIAELTNLLSLEKGKAKTVEDDLAALQATLATLRDENARLSG
                     LVAERDEQLRSGQGRISTLTASLDEQTKISNEALAKVDLLNQQLLALRRQIAALNEAL
                     EASERKDQEAQDRIKDLGQRLNAALARQVQELQRYRSDFFGRLRELLKDRKDIRIVGD
                     RFVFQSEVLFPSGQAEMTPEGLAAIDQVATAILELERSIPSEIDWALQVDGHTDSRPI
                     SSFQFPSNWELSTARAISVVRYLISRGVSPKRLIAAGHGEFQPLEEGDSEEALRRNRR
                     IELRLTNR"
     gene            complement(38852..40666)
                     /locus_tag="VF226_17895"
     CDS             complement(38852..40666)
                     /locus_tag="VF226_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX7075913.1"
                     /translation="MRRPRRSKAELAEPGEDTAEARRRSLRPLLKLKPYVFKHPGMLV
                     LAAIALLMAALATLVLPIAVRRVIDYGFGGADGLFIHRYFSMLMFIGFVLAVASAARF
                     YFVNWLGERVVADLRTDVFRHLTRLGPEFYDTTHSGEVTSRLTADTTQIKAAAGTAIS
                     QALRNAIMLVGALAMMFVTSAQLSFLVLVAIPVILLPLIAYGRLVRRLSRKAQDTLAE
                     ASALATENLGAVRTMQAFTSERTVSAKFAGAVETAFEAARARLKARAGLTAMTMFLVF
                     ASVVGVLWFGADAVVKGYITGGRLSQFVLYAVFAAAALAQLSEVWGEVTQAAGAAERL
                     AELLAIEPQIRTPDNPIPLPVPGRGEVAFHHVSFRYPSRDESALEDVTFEVGAGETVA
                     LVGPSGAGKSTIFHLLLRFFDPTAGVIRVDGVPIAEADLEQLRARMALVPQDVALFAD
                     TVAGNISYGSPDAGMDAVVRAAKLAQAHEFISALPQGYDTLLGERGVLLSGGQRQRIA
                     IARAILRDAPILLLDEATSALDAESEVSVQKALEQVMQGRTTLVIAHRLATIQKADRI
                     LVLDEGRLVEEGTHATLVRKGGLYARLAELQFAGEAAQ"
     gene            40896..41120
                     /gene="rpmE"
                     /locus_tag="VF226_17900"
     CDS             40896..41120
                     /gene="rpmE"
                     /locus_tag="VF226_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX7075914.1"
                     /translation="MKRDPDLHPDYHQIKVVMTDGTEFTTYSTYGKEGDTLTLDIDPK
                     THPAWTGGDQKLVDRGGRLSRFKKKFEGLF"
     gene            complement(41251..41802)
                     /locus_tag="VF226_17905"
     CDS             complement(41251..41802)
                     /locus_tag="VF226_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX7075915.1"
                     /translation="MGSKSSLRDEPSACVTISFGERFAASEQFEAVYAQGMGLVERTA
                     AYLDGAGRQEAKQLKPNVALTYSTESMRLTARLLDLASWLLIRRSLRDGDITQEEARR
                     KRARLKLRTSGRPSHIPNFNELPEQLQQLINESFALSDRIIRLDRSLEQVTATGDAAP
                     RPSKNPVAAQIAELQAAFNAAIR"
     gene            complement(42268..42456)
                     /locus_tag="VF226_17910"
     CDS             complement(42268..42456)
                     /locus_tag="VF226_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX7075916.1"
                     /translation="MDWDEIRPKPGSTITVGEPLHALSLSDLQERLTALREEIERVEQ
                     EIAAKRAHEQAASALFKR"
     gene            complement(42574..43434)
                     /locus_tag="VF226_17915"
     CDS             complement(42574..43434)
                     /locus_tag="VF226_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX7075917.1"
                     /translation="MTKAPRPPRLLSSIAPLVDGYDAWICDVWGVMHNGVRAFSGAVE
                     ACRRFREGGGTVLFLTNAPRPAHAVAAQLDGLGVPREAYDGIVTSGDLTRKLISERAQ
                     LKLFHLGPARDKPMFEGLKLRFTEAAEADVIICSGLYNDETETPADYTEMLEPLARRG
                     VPMICANPDLTVERGSRIVYCAGSLAAEYERLGGEVTYAGKPHLPIYEMAFERLGELR
                     GEPVPRRRILAIGDGLRTDIRGAANAEIDAVFIASAIHVPGELDAVAVAKLFDNTSLQ
                     PIAALPALVW"
     gene            complement(43467..44561)
                     /locus_tag="VF226_17920"
     CDS             complement(43467..44561)
                     /locus_tag="VF226_17920"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX7075918.1"
                     /translation="MSEDDAEPAGVEGDVEVIQDMIRMFSEEIAHPRKPSSDEAADER
                     TGSGEPAEADEAAISASVDALRAAAEEMRRPVEGGRASIEKGVLPPLRVDPNSSKPQP
                     PPVGPEHEDMAAMADAIATGKLDVFLEPIVGLADRKARHFEISLRLRNGDAGSFGPED
                     YIPMARNAGLLPQVDATRAARAAMVARHMDERGSGGCLFSSITAESLTSPRFLEEFAD
                     ACRQAPKLRERLVFSISQSELQGLSAAQWSTIRQLATSGFRFAIEDVTSLDLDLPDLK
                     AAGFAFMRINAQSMLDGLASLEGVLPISELCQRISEIGLTLIAVGLGGEDQLESVLAA
                     GVPLGQGQLFGVPRPIRAEALRPAASSAAA"
     gene            complement(45587..46915)
                     /locus_tag="VF226_17925"
     CDS             complement(45587..46915)
                     /locus_tag="VF226_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7075919.1"
                     /translation="MAPLDMKPNELESYWLPFTPNRSFKKKPRLFSGAKDLHFLTPDG
                     RKVIDASSGLFCVNAGHGREPIVKAIQEAAARLDYAPPFQYGHPRVFELSSRLSLLAP
                     GDLEYTLFANSGSEAVDTALKVALAYHRSRGEGSRTRFIGRERGYHGVGFGGISVGGM
                     VANRKMFGNMLTGVDHLPTTYNRSKQAFSVGEPEWGAETADELERLIGLHDASTIAAV
                     IVEPVAGSTGCLPPPKGYLERLRQITEKHGILLIFDEVITGFGRLGYPFAAERYGIVP
                     DMITFAKGVTNGSVPMSGVMVRKPIYETHMQGPEHIIELFHGYTYSGHPLAVAAGLAT
                     LDVYKDEGLFEKARANEKAFADAMMSLRGLPNVVDIRPIGLICGIDLDPIPGKPGLRG
                     YNAIESMYHEHDLYVRVSGDTICVAPPLIATESDFGQIRDKIATVIKAVA"
     gene            47124..47675
                     /locus_tag="VF226_17930"
     CDS             47124..47675
                     /locus_tag="VF226_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7075920.1"
                     /translation="MTSVDVGGRLRYLRSRHNLSQRELAKRAGVTNSTISLIEANRVN
                     PSIGALKRILDGVPIGLAEFFALEPEKPRQVFYMAEELSEIGKGGISYRQVGENLVGR
                     ALQILKERYEPGADTGRVPLVHEGEEGGIIISGRLEVTVGSERRILGPGDAYYFESRR
                     PHRFRAVGPVPCEVVSACTPPSF"
     gene            47893..48645
                     /locus_tag="VF226_17935"
     CDS             47893..48645
                     /locus_tag="VF226_17935"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7075921.1"
                     /translation="MLTSVLESGVCVGSYPELAGKRVLITGLNLKAGVDIARAFAEQG
                     CRIVLHTTASGTEADAVLEMLAQTAAELQVYSDPLTDVAATTKFAQTAARTFSGLDAV
                     INLLHADVAAVVAQAADADIESKIASHLQNARLITRIAANRMRLTWSEGLILNALVAA
                     PPVTGAEAMVLGITRSMLASMTRAEAQQWADQAIRINAIAPSTDGPDGCEHLASEPDI
                     ALLAMFMASPRGREFSGLVFDTALAGNAREAA"
     gene            complement(48697..49854)
                     /locus_tag="VF226_17940"
     CDS             complement(48697..49854)
                     /locus_tag="VF226_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX7075922.1"
                     /translation="MKFTGNWNYPTSIRFGAGRIAELADAVKSAGISRPLLVTDPVLA
                     KLPITTRALEVLEAGGVPAKVFSDVKPNPVAANVEAGVAALKAGDHDGVVAFGGGSGL
                     DAGKVIAFMAGQTRPMWDFEDIGDWWTRADPKGILPIVAVPTTAGTGSEVGRAGVITD
                     EATHTKKVIFHPLMMPKVTICDPELTVGMPKAITVGTGMDALAHCLEAYCGPFYHPMA
                     DGIAVEGMRLVKENLVRAATNGQDIEARAHMMSAAAMGATAFQKALGAIHALSHPVGS
                     LYDTHHGMTNAVFMPYVLMFNRPAIEDKIARLAAYLGLKADFNAFLDWVLQIREETGV
                     PHTLAGLGVDGSKFDIMSRMAPKDPTAGGNPVPLDEAASRELYEKALSGKL"
     gene            complement(49974..51356)
                     /locus_tag="VF226_17945"
     CDS             complement(49974..51356)
                     /locus_tag="VF226_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7075923.1"
                     /translation="MPEIVCISPIDGREVARRPTASAAEIERALDAARTARQEWVRVP
                     VRERAALMLRFLEAMRAINPQVTRELALQMGRPVRYGGELGSFSERVAYMAEIAEEAL
                     APIVPEPKAGFKRYIRRDPLGLVLVIAPWNYPYLTAVNTVVPALIAGNAVLLKAASQT
                     ILTGERFQQAFDQVGLPKGLFQNLVLGHDDTARILGDGEVDHVTFTGSVSGGRAIERA
                     AAGSFTSIALELGGKDPAYVRSDAKLDHAIENLVDGAFFNSGQSCCGVERIYVDERIY
                     DPFVEGFVALTKQYVLDDPLDEATTLGPMAATRFADVVRQQTAEALRKGAKAHIDPAS
                     FARNKDGTPWLAPQVLTNVDHGMQVMKEESFGPVVGIMKVSGEAEAIHLMNDSPYGLT
                     ASVWTTDLEAAERIGQQIETGTVYMNRCDYLDPALAWTGVKDTGRGAGLSRIGYEMLT
                     RPKSFHLREV"
     gene            51486..51881
                     /locus_tag="VF226_17950"
     CDS             51486..51881
                     /locus_tag="VF226_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7075924.1"
                     /translation="MKFLAPYEPQLLSVLRFMSGLLLLQHGTTKYLNIPVGPMNNASP
                     FTMSGAAGVIELIGGVLLVIGLFTRPAAFIASGMTAVAYFYAHAPRGFFPILNGGELA
                     VLYCFVFLYLAAAGGGRWSIDWMLNRKDR"
     gene            complement(51931..>52206)
                     /locus_tag="VF226_17955"
     CDS             complement(51931..>52206)
                     /locus_tag="VF226_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX7075925.1"
                     /translation="FAALLAAGLAGIDEKLELDPPFSGDAYVRDGIPEVPKTLREAVD
                     CLDKSEFMREALGSAVVDHYVHTGRWEQFEYDRRVTDWELMRGFERY"
BASE COUNT         9900 a        16614 c        16231 g         9461 t
ORIGIN      
        1 tttccagccg catgatgacg ggaatcatct ggaacgtcga tgcattgccg acgccggaca
       61 tgaaaaacaa gaaaagaaaa ctcagcagaa acagggtgaa gctgccaatg ctcaaggcat
      121 acaagacccc aagcacgccg acgctcatca gaacgaaaac ccagaacgta acgcgtgcgc
      181 cgccataccg atctgcgagc cagccagtgc ccgagcggac gagagccccg atgagcggcc
      241 cgaggaaggc aatccgtaat gcgtcgacgt ctggaaactg gctttgcgcg agcagcggga
      301 agccggcgga aaatccgatg aacgagccga atgtgccagt gtaaagccag cacatgatcc
      361 agttgtgctt gcgttggaaa atgatcgact gctcggcaaa cgacgactgc gccgtttcga
      421 tgtcattcat tccgaaccag caggcgaacg ctgccaaggc aatgaatggt acccagatat
      481 agccggcatt ctgcagccag atcggcatca ccccgctttc ggtttgcacg aactgcggtc
      541 ctccgccgag ccaaccgaaa atgcccaccg tgatgacgag tgggacgacg aactgcacga
      601 cagatacgcc aagatttcca agccctgcgt tgagcgccag cgcgtggccc ttctcgcttc
      661 gcgggaaaaa gaacgaaata ttcgccatgg acgaggcaaa gttgccgccg ccgaggccgc
      721 aaagcaacga caacagcagc agaacccaat agggcgtctc agggttttga acggcgagcc
      781 cgatgccgat cgcgggaatc atcagcgacc acgtcgtgag agtggtccaa agccggcccc
      841 cgaacaccgg gaccatgaag gcatagaaga tccgtaagac cgctcccgac aaacccggca
      901 gcgctgccag ccagaacaac tggttcgtcg acagcgggaa acccacagcc ggcaatctcg
      961 cgacgatgac cgaccagatc atccagacgg caaacgacag gagcagcgcg ggtatcgaca
     1021 gccaaagatt gcgcttcgcg atggcccgcc ctttgtcctc ccaaaattcc tcgttctccg
     1081 ggcgccagtc cgtcagcagg tgtggcccca gaacgccgat atgtcgaggc tcgtgcacct
     1141 cctgcatctc gggcaattcg ggcagcttcc ggagctccgg cgcaagcacc ccgcgctcca
     1201 tcgccctgat ggagacgtgc atccagatca aggcgagcgc cgaaatggcg aagagcaaca
     1261 tgaagacggt ggtccaaata cccgtgaggt cgaccaggat gccaaaagcg atcggcagga
     1321 cgaaaccgcc caggccaccc accatgccga ccacgccccc gaccgcgctg acgttctcag
     1381 ggtaataaac gggaatgtgc ttgtagatgg ccgccttgcc gaggctcatg aaaaacccga
     1441 ggacgacaag caatgccgtg aaagcagtca acccgatttc aaaatggaat gagattggtc
     1501 ctctcgcgct cctcacgatg tattctgtcg gcgggtagct gaggatcaga cacaccgctg
     1561 tcgacacgcc gaacgtccag tacatgacgc gccgggcgcc ataggtttca gacagccgcc
     1621 cgccgtagat ctgaaacacg ctggcaggga tcgcaaaagc cgaaccgagg acgcccgcag
     1681 tctggagatt gaccccgtag gcaccgatca gatagcgcgg tagccacaag gcgagtgcga
     1741 cgaaggcccc gaacacaaag aagtagtaga gcgaaaaacg ccagacttgc acgcggcgca
     1801 gcggcgccag cattgcggcc agcgtcggcg gcggctcacc ggttctctca cgcgccgcaa
     1861 tcggtggctc atcccgagcc atgacccaga acgcgaccgc catgacagcg agggccaacg
     1921 cccagatgcg cgcgacgccg tgccagccgt aggccgccaa cacgaacggc ccaagaatca
     1981 tcgtgactgc ggcgccgaca ttcccgagac cgaaaatatt caacgttgaa atctgctgtt
     2041 caggcgaata ccatttcgac agatatgtca cgccgacgcc gaacgacccg cctgcgattc
     2101 cgacagcgac acccgcgagc agtatcgtcg gatagctgcc tgcaaatgtg agtagccagg
     2161 ctcccagcgc tcccccgagc atgaccaggg agtacacgac gcggctgcca tatttctcac
     2221 tccaaatggc gaccggaacc cgaatgaggc tgccaacagc gatcggcaaa ccgacaagca
     2281 aaccgaaaag tgtttcgtta aggccaagct cggccttgat tcgaatgccg atgatggcga
     2341 agatcgtcca gactgcgaac gaaatcgcga aggccagggt cgaaagccaa agagcgcggc
     2401 cttgattgag ccggcgcacg tctcgttgcc ctacgtccgt cctaccagtc atcacagttg
     2461 gcgaagttga atgtcgaggc ctcgcgtgaa ttgctggagc ctacgtctcc agcgctaagt
     2521 cggctcaacg ccaaaattcg gtggaactcg cggtgccgcg ccgcagccgc gtcgcgggcc
     2581 gcaaccggag aattctcggt cggcgggctc gtcggtctcc tcgatgatcg cagtggcctt
     2641 gagcctcgca atgtagttgt ggactgcctc gcgccaagtc ttttcgcgga ggtaggcttt
     2701 gatccggtct ttcacgcttt catagggaag ctgccggccg ttgagccgct gattcaaccg
     2761 cacaacatga tatccatagc gggttctgat cggcacaggg cagatctggc cgggctccag
     2821 cgcgaagagg aaggtttcaa actcgggaat tgtggagcca cgcgtgatct ggccgaggtc
     2881 accgccgtgt tcccgcgagg agcaatcaga atgctcacgc gcgagctcgc caaatctggc
     2941 aggatcatca cgaagctgct tcagcgccgc ctcggctcgc gccttggcct ttgccatgga
     3001 ctctcggtcg tttggatttg ccgagaacaa aatgtgagcc gcttgcagca gatccgggct
     3061 tttgaatcgt tgcgggtttt caagataata ggcgcggcag tcctcctcgc ttggctcggc
     3121 tacttcgatc tctctatcaa ccaatgcacg gatgagcgcc tcttcaggcg cttctaccaa
     3181 ctttttcgtc gcatccgtca gtggacgcgg agcggaaatt cccaggcgcc gcacttccga
     3241 aagcagaagt gtccgaacca ccagggcatt aactgcttcg cgtcgcgcgt cgtccggcga
     3301 gggcgccggg tggtactgca tttcctcccg gatgcgctcc tctgtaatct cgacgccgtt
     3361 gacgcgcagg gccattcgtc agactccccg cttggaaaga ttctggtttc gcctcacgat
     3421 ctgccatcca ggcctgccca gcatccaaat gggtgcgctg aagatatgca cgagccgcgt
     3481 aaagggcgtg atcagaaaca gcgtcatccc gaagataagg tggatcttat agatccaggg
     3541 cacattctcg agcagagctg cacttccagg ctggaaactc aaaatgctgc gcgaccactc
     3601 cgccagaagc agcatgtagc tcccgtcaat gttctggtaa gagacaggga tgcccgcgat
     3661 acctatgata agttgcacgt agatgaagag tattatgaac agatcggtcg gtttactggt
     3721 cacgtaaatg cgcgactccg aaattcggcg gtaaatcagc atcgtggccc caatgaagca
     3781 tatgatggcc agcacggcac caacgatgat ggcaagcatc tgctttgcct tcgtacttag
     3841 ccccaatgcg tgataaaccc acggtggtgt gagcagcccg aaaaagtgac cgaaaaacag
     3901 ccctatgatg ccgatgtgga agagatagct gccccagaac agatagcgct tttgcaggaa
     3961 ctggctggac tggctgcgcc aagtgaattg gctgcggtcg aagcgcgccc aagcgccgag
     4021 aaagtaggcg accatacaaa gataggggta gaacccaaaa aagaattgat ccatccaact
     4081 catcgggatc acctcaacgc tcgatttcac ccgtggattt acctggagga tctgcgcccg
     4141 caatctcgcg ctccatgcgc tccaccatcg ccgcggcaat ccggcattcg ccggcagctt
     4201 gatcactgag cggcaagtcg aaccggaccg gcgcctcctc ccattccttg tcgagatcga
     4261 cgggctgctc tgccgccacg gctgcagcga cagcagccgg atcgacatga gctttcgcta
     4321 tggcgcagag aacccggaat accaccgcat actggctgga ccgttcggaa aggcgcgcct
     4381 gcagcagcgc aaggatgtct gcaacttccg ccagcattgc tcgcgcgtcc tccagcggct
     4441 gcatcgacag gaattccagg aacagtggca ggtaatccgg cagctcgtcg gcggtgattt
     4501 ccagaccacg cctcctgtac tgctcgatca ggtccgacat cgcagcgccc cgttcccgcg
     4561 aatccccatg gatgtgctcg aacaggtgca gggaaagaga gcgcgagcag tcaaacagcg
     4621 caacgtattc tgcctcggca tccagcggac tggccactgc ccgccggtcc atcagactgc
     4681 gcaacccttc gataagagat tcgttgacaa gaccttcgcg ctcgagaatg gcgagcattt
     4741 cggcgcgcgc ctgattcagc tcggacgtcg gatagaggag tagtcccgac aataccttga
     4801 acgtgagcat ctgctttctc ccccctactc gaagcgctcg ccgcgcgttc ttcgcgtatc
     4861 ccggccgaaa aggctgaagc tggagtttcc ttccgaacag ttctgggtga aattgaagcc
     4921 gcaaaccccg cgttgatcgt aggcgtttct caaggcggtt tccttgtggc tggttggaat
     4981 gacgaagcgg tcctcgtaat tcgcaatcgc catatagcgg tacatttcct ccacctgatg
     5041 ttgcgtcagt ccgacccgct ccagcacgcc gaggtcgagc atgccgtcga tctgcttgcg
     5101 gcgcatgtag gcacgcatcg ccagcatgcg ctccagacag cggcggatgg gtgcctcgtc
     5161 gcccgccgtc atcaggttgg cgagataccg aatgggaata cgtaagctcg agacgtccgg
     5221 aatgccgtgc agcgatgcga tagcgcccat ttcagcggca tgctggatcg gcgaaagcgg
     5281 cggcacgtac cagaccatcg gcagcgtgcg gtattccggg tgcagtggaa atgccacctg
     5341 ccattccttg gccattttgt aaaccggcga gcgttgcgct gcttcgatcc acgattccgg
     5401 tacgccgtca cggcgcgcct gctcctgcac atcgggatcg tacggatcga ggaaaatatc
     5461 gagctgtgcc tggtaaagat ccttctcatc cgccaccgac gccgcctcga ggatgcggtc
     5521 ggcatcgtag agcattacgc tcaaataccg gagacggccg acgcatgtct cagagcacac
     5581 cgtcggcagg cctgcctcga tgcgcgggaa acagaagatg catttttcgg atttcccggt
     5641 atgccagttg tagtaaatct tcttgtaagg gcaccccgtg acgcacatcg tccatccgcg
     5701 gcatttctcc tggtcgatga ggacgatgcc gtcttcctcg cgcttgtaaa ttgctccggt
     5761 cgggcaggcc gcgaggcagg acggattgag gcagtgctcg cacaggcggg ggagaaacgt
     5821 caggaacgta ttttcgaact ggccgtagat ctctttttcc accccctcga agttgtagtc
     5881 ctcggagcgc ttcgaaaact ccgttccaag gctctcctcc cagttcggtc cccatcttat
     5941 tttttgcatt cgctcgccgg tgatcataga caccggccgg gacgtggggg cggcttccga
     6001 ttcgggcgca tggtggatgt aggagtactc gaaatcgaac ggctcgtaga agtcgtcgat
     6061 ctgcggcacg tgcggattgg cgaagatctt ggctagaatg cgccatttcc cgccgatacg
     6121 cggctcgatc gtcccatccg gcttgtagcg ccagcccccc tgccaacgac gctgattttc
     6181 ccagtcctta ggatagccga tacccggctt cgattcgacg ttgttccacc aggcgtattc
     6241 gacgccctgg cgcgtggtcc agacctgctt gcaggtgatc gagcaggtgt ggcagccgat
     6301 gcacttgtcg agatttagaa ccttgccgat ctgggcccga attttcatta cctgagctcc
     6361 tccgccataa gcgggcgctt ctcggcgagc gggcggtcgc gccaatcgag ctcacggatc
     6421 ttgcgcagga cgatgaactc atcgcggttc gatccgaccg ttccgtagta gttgaacccc
     6481 caggaaagct gcgcatagcc gccgatcatg tgaaccgggt tcatgaccac gcgcgttacc
     6541 gaattgtgaa tgccgccgcg atttcccgta atctcgctgc caggcacatt ggccatgcgc
     6601 tcggccgcgt ggtacatgaa ggccgcccct tccggaatgc gctggcttac gaccgtacgg
     6661 gcgaccagcg ctccgttgag gttgtaggct tcgatccagt cgttgtcctc gataccagcc
     6721 ttttgcgcat ccttttcact gatccacaca atcggcccgc cgcgtgccag agtcagcatg
     6781 atcaggtttt cggtgtaggt ggaatgaata ccccatttct gatgcggggt gagaaagttc
     6841 aaaacgattt gcggattgcc gttgtcgtag cgctccatca catcatcgag ggagcgcgta
     6901 ttgatgggcg gcttgtaggt cgccaggctt tccccgaagg cgcgcatcca ggcgtgatct
     6961 tgataaagct gctggcggcc ggacaaagtc cgccagggga tcagctcgtg cacattggtc
     7021 cagcctgcat tatagctgac ctgctcggat tcgattcccg accagatcgg ggacgtgatg
     7081 atcttgcgcg gttgtgccac taggccgcga aaccgaatgt gttcgtcctt acggaaaacg
     7141 gcgagatggg tatgttcccg ccccgtcgat ttctcgaggg cctcccaggc cttcaccgca
     7201 acgtgaccat ttgtttccgg cgccagcgtc aggatggact cgatcatgtc cacgtctgtt
     7261 tcgaggcgcg gctgaccttc tccgactcca tgagcctgtg tcgaattcaa ggagcgcagg
     7321 agcgcaacct catcagccgt gctccacaga attcctttca gcgaattccc attgcgttcg
     7381 agcagcggac ccaacgattt gaatttctct gcgatggccg tgtagtcgcg tttgacgacg
     7441 gtcatctgag gcatggtctt gccgggaacg ggatcgctgc cgccgtgccg ccagtccttc
     7501 ccgctgaacg gctgcgcaag ctcactcggc gaatcatgca tcaatggggt cagcacgatg
     7561 tcctcgacct cgccgaggac gccctgggcg acgcgcgaga aattttcggc aaatcccttg
     7621 aagattgcga agtccgagcg cgattcccag accggatcca ccgcggagca gagcgggtgg
     7681 atgaaaggat gcatatcgct ggtgttcagg tcgttctttt cataccaggt tgctgtcggc
     7741 agcacgatgt ccgagaaaag gcacgtcgtg ttcatccgga agtcgagcgt gacaagaaga
     7801 tcgagcttgc cttcgggcac cggatcgcgc cacgtcacct ccactggctt gagtccgccc
     7861 gtcgtgccga tgtcctcgtt cagaacatta ttccgcgtgc caagaaggtg cttcagaaaa
     7921 tattcgtgcc ccttgccgct tgccccgaga agattggcac gccagacgaa aagattgcgc
     7981 ggaaagtttt tgggattgtc cggatcctca caggccatcg agagctgacc ggatttcaag
     8041 gcttcgaccg tataagcaac gggatccttg cctgcctcac gggcatcgcg gcacaccttc
     8101 agcgggtttt cgttgagttg cggggcggac ggcagccagc ccatgcgttc ggcacgcacg
     8161 ttgcagtcga tgaacgtccc cttgaactct tccgggtcgg caagtggtga gagcatctcc
     8221 gagaccttca ggcgctcata tcgccattgg tccgtatgcg cgtacatgaa tgaggtgccg
     8281 ttcatcagcc gaggtgggcg gctccaatcg gtcgcaaacg cgatcggaaa ccagccggca
     8341 aggggccgca gtttctcctg gccgacataa tgcgcccagc cgccgccgct tttgccgacg
     8401 catccgcaaa gcaccagcat attgatgatg ccgcggtagg tcatatccat gtggtaccag
     8461 tgattgagac cggcaccgag gaccaccatc gaccggcctt ccgtcgcctc ggcattggcc
     8521 gcaaagccac gggccgtatc gatgatcttc tggcgtggca gtccggtgat cgcttcggcc
     8581 caggccgggg tgtagggcgc attgtcgtcg taggacgatg cgatgtctcc ccctagtcct
     8641 cgatccaccc cgtaatttgc caccatcaaa tcgaagacgg tggcgaccag ctcctcgccg
     8701 ccgttcagcg atagctttcg cgcagggacg tttcggacct gaatttcgtc gtgatcactc
     8761 aatccgaatc ggcctggtgc gcggctgccg aaatagggaa atgcgacggg aacgatctcg
     8821 tccgcgctgt tggcaagtga caagcgcagc ctgatgggac ggccatcgcg gccgtccttc
     8881 tcctccaggt tccatcggcc aggttctgtc tggggctctg gcgcttcccc ctgcgcggct
     8941 tgttttgccg acctttccca tcgaaagccg atcgagcctt gcggcacgac aacgtcgctg
     9001 ctgctctcgt cgatgccgac cattttccac tcgggataga tgtcttggcc cagcttgtca
     9061 gcaaaatcgg aggctctgat cagggcgcct ggaacgtatc tatcgccccg cttttccagc
     9121 cttaccagca aaggcaggtc gctgaagcgg cgcacatagt cgtcgaaata aggtgtggtc
     9181 ttttcgatat agaattcttt cagaatgacg tggcccatgg cgagcgcgag agcggcgtcc
     9241 gtcccctgct tcggcgcgag ccaaaggtct gcgaactttg cacactcgat gaaatccggg
     9301 gcgatgacca cgctcttttg gccgcgataa cgcgcctctg tatagaagtg cgtgtccggc
     9361 gagcgagtca tggaaatatt ggacccccag acgagcagga aaccggcatt gaaccagtcg
     9421 gcgctttcgg ggacatcggt ctgctcaccc caggtctggg gcgaggatgg cggcaggtcg
     9481 caataccaat cgtagaagct gaggcaagtg ccgccaatga gcgacaggta ccgcgtcccg
     9541 gccgcatagc tcaccatcga catggccggt atcggcgaaa acccggcaat gcggtcggga
     9601 ccccaggttc tcaccgtgta aacattggcc gctgcaatca gttcgttgat ttccgaccaa
     9661 tccgctcgca cgagcccccc aagtccgcgg acgcttttgt agtgatttgc ccgctcggga
     9721 tattcaacga tcgaagccca ggcctcgacg ggcggtaatg tcgctcgggc ctcgcgccag
     9781 agctggagca acctggcgcg gatcagcggg tattttatgc ggttcgagga atagatgtac
     9841 cagctgtagc tggcgccgcg aggacagcct ctcggctcgt ggttgggcag atcgggtcgc
     9901 gtgcggggat aatcggtttg ctgggtttcc caggtgatca gtccgttctt gacgtaaatt
     9961 ttccacgagc acgagccggt gcagttcacg ccatgtgtcg aacgaacgat cttgtcgtgc
    10021 tgccagcgcg tccggtaatt gtcctcccag gccctgttct cgttggtaac gaccccgtgc
    10081 ccatcagcaa attggcccac atacttccgg aagaacagga gccggtctat gaactgactc
    10141 atgagcgtcc ccgtgctgga gcgaattcgc gatcgcctgc cttggggcta acacgggcgg
    10201 cagcgattcg ttcctgaagg gaacggtcga cggtgtctgg ccatcgcaat cagatccaag
    10261 cgccgacgtc ctcgaatgaa aatgaaactg gtaaatgcct gtttcggctc gcgccaacgc
    10321 tcgacttgat gggcgccgtc caggggccag ccgacggagc ctcaaatgac ctcgaaatat
    10381 gcactcatgc cggccatctg atgctccagc atgtggcagt ggagcatcca tttgccggga
    10441 ttgtctgcga ggaatgcaac tgacatttcc tcccgcggct gcatgagcac ggtatcgcgg
    10501 aaccagggct tgagcgtgcc gcgagtccgc gagatcgtca gcatgtggtg gccgtgaatg
    10561 tgcatggcgt gaggccaagc cgtagcgttg gtcatcttga taacgactgg gcggccgcgt
    10621 tcgacggtaa agagcgggct tatgtggttt ccggccttgc cgttgaaagc ccaaaccatg
    10681 cggtgttttt gggcgaggtc gcggaggctg aagtatttgc cctcgtattc cgcgcctgcc
    10741 atgctgctca tggcaccacc ctccatcagc atcggaaaga cgagcggatc ctgtgagagg
    10801 tttggtacgg gaacaggatt gcgtggtagg gtgacaggcg tgtcaagagg cctttcgcga
    10861 gcgcgcacgt catccgccaa tacgaatctc gcgattgaaa taggctcacg cgtcacttcc
    10921 ctgaccgaga cctgtgagcc gggctcacct gtcaggtcga ggacgacgtc ggcgcggccg
    10981 gacgggggta ggacgaaact ggcaccgccg tccatcagct caggactgat aggctgccca
    11041 tcgacggcga tgacttgtgc acgcgcattc tcgaactgca gctcgagaat tcgcgaattg
    11101 catgcgctgc aaagccggac ccgaagccgt tctccggaac gcgccggcag cgtgggcgcg
    11161 acttggccgt tggcggtcag cagattgccg atgcggccgg cgtgggcggc gtcgaacaga
    11221 tgggccaggc tctgcgtctc gattgagccg tcagcattca gccgccaatc gtcaatgatg
    11281 agcgaaacgt cgcgatcgac cgcaaccggc ttcggttcct cgacgatgag caggccgtaa
    11341 aggccgcggt cctgctgcat gacctgatgc gcgtgcgggt gataccagaa tgtgcctgcg
    11401 tccggtgctt tgaaacggta gtcgaacgac tggcccggct cgacgggagg ctgcgtgaga
    11461 tgagctacgc cgtccatggc attgtcgatg cggacgccgt gccaatggat cgtcgtcggc
    11521 tcgccgatgc cgttgctcag ccgcgcgaag acctcgccac cctgccggat gcggatttcc
    11581 gggccgggaa ccgtgcctcc ataaccccag atacgcgttg gttcaccttg tgggagaaac
    11641 cgggcggcgc caggctcagc actcagcgaa atggggcggc ccggcatctc cgcgtgcaaa
    11701 ggtcgaggca gcagcgcata tccgcccgcg ccggccatcg caccgagcaa tgtgcgacgc
    11761 gagatcgagg cacccgaccg gattgggatc atgttcagct ccgcggcttg ccgttcgctg
    11821 gcatcttccc catggtcgac atttcagcct atgagttcaa gcggcatagg ctgtaaggtc
    11881 gggtgagctg ccaattgaca ttcgctgatc cggcaccggc tcgtcgacag ggggcgcgcc
    11941 tctgctttcg ccttcggacc atcgatagat ctgtggaggc ctgccctcat gcccggaaag
    12001 cggggcaaga tccggattgg tgttggggga tggacgttcg agccctggcg cggctcgttc
    12061 tatccgcccg gcctacctca caagcgcgag ctcgagtatg ccagccgcaa gctcacctcg
    12121 attgagatca acggcacgta ctacgggtcg caaaagccag aaattttcgc caggtggtac
    12181 gaggaaactc ccgacgactt cgtgttcgca gtgaaaggcc cgaggttcgc gacgaatcgg
    12241 cgcgtgctgg cggaggccgg tccgtcgatc gagcgcttct tcgccagcgg agtcatggag
    12301 ctgaaggaga aactcggccc gatcaactgg cagttcatgc cgaccaagaa gttcgatccc
    12361 gaggatttcg cagcctttct caagctgctg cccacgcgca tcgacggtcg cgcgatccaa
    12421 cacgccgttg aggtgcggca cgagagcttc tgcacgcccg atttcattgc cttgcttaga
    12481 gcgcatggcg tagcggccgt catagccgga gactcaaaat atccgcggat tgccgatctc
    12541 acggctccgt tcgtctacgc ccggatcatg ggaacgagtg aagccgtgga aaccggctat
    12601 aagccagcgg agctcgatca atggtcgaag cgcgcgaggg cctgggcgga gggctgggtt
    12661 cccccggggc tccacactgt cggccccgcc aagggcgacc gcaggccacg cgatgtcttt
    12721 ctctacgtca tcagcggtgc caaggtccgt aatcccgccg ccgcgctcgc actgatcgag
    12781 cggatcggca aggcgggaag ctgaaacggt tgcgattcag ctcaagtcag ccgtggattc
    12841 gtgggacgcc acaaagacct tatagacgag ggcctcggct tcgcgcttcg cgacaggccc
    12901 tgacatcggc ggcgcccctg cagcgtcccc tcccaccccg cctcgccttg gtggactggc
    12961 tagtggactg ctgccgacca tcgctacacc ttcggccgtt gcaatagcgt tcggggggtg
    13021 gcaccaacgc gtttcgcgaa cacttgtaag gccagacgat tttggcttcg ggcggtatga
    13081 cctcaccgct gtccaacagc acagcgatat cgacttcctt ctgcgacgtg ggcagcccct
    13141 tcatggaatg agagtcgatc ggtgtcagtc ctcgataaag ggccacggga tctgcccatc
    13201 ggccatccac cggaaccacg tgggtacccc tgacaatagt atactggctg ctctccgtaa
    13261 aaatggccgc aaaatgaagc gctggccggc ggggccgatt caccctcttc tgtcgctctc
    13321 ccctgctccc ggaattgcca gtctcgccaa ggacttcacc catggccacc cgttggccga
    13381 cctccacttg tggttcggaa cgaaagtgcc cgtacgacgt gtagatccag agcggcaaac
    13441 ccgtgtcgtc aggactgtgc tggattataa cttcaattcc acgctgggtt cggtccgggt
    13501 cggtgacttt ggccaccacc gttcccgctg cggcagcaac gatcgcctca ccccatggtg
    13561 ccggcatgtc gattccgccg tgataggatt cgcgactacg cttgtgcgtg taggaaatgg
    13621 cccagtgctc gtcgatagtt cgacaattga tgccggttgg gaaccgcggc cgaagacctg
    13681 tttcgaaaag gccccgcttg acagccgcat cgatgctgca gtcccgtgac cgagtgatcg
    13741 cacagatcgt ttcctcgtcg gggctttcat tcgccggctc cgattgcgcc cccgcgggat
    13801 cgctcaacaa aaggatgagc agcacgccaa taaggaacgt tgcacaccgc atcgcttccc
    13861 ccctgaaccc atcacggtga aactccccag caatcccggc attatctatg ggcagtacac
    13921 gttgtaaatg gcggatccgg cgcagctgag gtcaaggaga gtgctgaagc cctggcggca
    13981 ccttcatcgt cgtttggtca ctgcaaaccc ctattcgcgg tcaacgcttg ctccaggtcg
    14041 gagatgagat cctcgaccgc ttcgatccca accgaaagcc gcagcaagtc cggagggcac
    14101 gggctgccct cgccctccac actggcgcgg tgctcgatca ggctctcgac gccgcccagc
    14161 gaggtggcac gtttccagac cgtaaccctg gccgccgccg caatcgccgc ctgctcgccg
    14221 cctttgacgc ggaccgacag cattccgccg aacccacccc gcatctggcg cgcggcaatg
    14281 gcgtggccgg gatgcgtttc aaggcccgga tagagcacgt ccgcgatctg cggatgcctc
    14341 gcaaagtgct gagctatggt catcgccgca gcgcattgcg cattgacccg caaatgcagc
    14401 gtgcgcaacc cgcgcagcag taatgcggcc tccatcggac caagtatggc gccgtggcgg
    14461 ctgcgtacgg cgacggagcg ttcgaaaagc gtctccgaag cggcaaagga caggacgccc
    14521 gcgatcgtat cggagtggcc attgaggtat ttggttgcgg aatgcatgac cacatcggcg
    14581 ccttcagcga gcgggcgcgt cagaatcggc gtcgcgaccg tcgagtcgac cgcgacccag
    14641 gctcctgccc gatgagcgat ttcggcagca cccctgatat ccgtgatcgt ccagagcgga
    14701 ttggctggcg tttcgatcca aaccagtctg gtttcgcccg ggcgcacagc cgcggcaatg
    14761 gcgtcgagat cggagctgtc gacgacgctc acggataagc cgtagttcgg cgcatcctcg
    14821 atgagccact ttctcaggcc ccagtacatc acgcgcggtg cgatgacgtg cgccggccgg
    14881 ggcagggcta gaaaaagcga tgtcgccgct gccataccgg aggaaaacag caggctggat
    14941 gccgctcctt ccagcgcggt cagcacggct tccgcttgac gcaccgtcgg attgtccggg
    15001 cggccatatg agtagcctct gcggtattgg ttgtccggat cacgcagata cgtcgtcgtg
    15061 atgtggactg gcggcacgat ggcgccggtt tcggcatcca ctgcgccccc tgcctgtgcg
    15121 agtatggttt gcggcctcaa gtctcgggac attcaagagc ccttgcgtga gatcgtgaaa
    15181 tagtgcggtg gtcaatgcac gagagcgagc ctatcacgag ttcaccactt cacgagttgg
    15241 cgacttcacg aaaatggctg accgaaccgt tctgatcacc ggttgctcat ccggcatcgg
    15301 gctcgcatcg gcgcggatga tgaaggcgcg cggctggcgc gtgcttgcga ccgcacgcaa
    15361 gccggaggac ctgaagcgac tcgaagcgga tctcgggctc gaggcgctcc atctggagct
    15421 tgcggatccg aacgccgttg ctgcctgtgc ccaagaggcg ctgaaccgga cgggcgggcg
    15481 gctttatgcg gtgttcaaca acgccggcta cagccagcct ggggcggtcg aggacatcac
    15541 ggcagaggcc ctgcgccggc agctcgaggt gaacgtgatc ggcccacatg atctgacacg
    15601 ccgtctgatc ccggccatgc gggccaatgg cgaaggccgg atcgttacct gctcgtccgt
    15661 gctcggtctt gtttctgcgc catggcgcgg tgcttactgc gcctccaagt tcgcgctcga
    15721 agccctcacg gacgcgctga ggctggagct tcagggcagc ggcatccagg tctcgctgat
    15781 cgagccgggg ccgatccgca cgcgcttcgt cgagaatgcg cgcgccgccg ccgtcgccaa
    15841 cgtcgatatc gagaagtccg ttcaccgcga gcgctaccac cggatgctcg cggcgctgga
    15901 gcgcgggggg cgtcaggcgt tcaagctgga gcctgaaagc gtcgcacgca ggctcgtgca
    15961 cgcgctcgaa agtccgcggc cgaaggcgcg gtactacgtc accacgccaa cctacgcggc
    16021 gtcgatcctg aagcggatcc tgccgacgaa attgctcgat cgggttgctg tccgcgcctg
    16081 agatttttca ctcggccggc tgcgcagcat tgacgttcgc cgcgggccga cgatgtgccg
    16141 tcagtttgcg tatgacgacg tagaagaccg gcgtgaacat caggccgaat agcgtgacac
    16201 cgatcatgcc ccaaaagact gccgtgccga gcgcgatccg catctccgcg cctgccccgc
    16261 tcgcaaagta gagcggcagc acgccaacgg tgaaagcgag cgaggtcatg aggatcgggc
    16321 gcaggcggag ctgccctgcc tcgacggcgg cggttacgct atccttcccc tgctcctcga
    16381 tctggcgcgc aaactctacg atcagaatgg cgttctttgc tgccagtccg atcagcacga
    16441 tgaaggcgat ctgggtcagg atgttgaggt cctgtccgtg cagccacact ccgatgagcg
    16501 ccgaaagcag gcacatcggc acgatcagca tgatggcgag cggcagcgac cagctctcgt
    16561 actgtgccgc cagcgccagg aacacgaaca cgacggacag gagaaagatg tagtagcccg
    16621 tatggccggc ccggacctcc tgataggaaa gatccgtcca ctcgtaggtc acgccctcgg
    16681 ggagcacttc ggcggccacc tggtgcatca cgctcagggc ctgccccgac gagatgcccg
    16741 gcgcgcctgt tccggtgatt tcgacggtcg ggaacaggtt gtagcggggc acacggtccg
    16801 ggccggacat ctcacggaac gtcacgaccg agttcagcgg caccatctgc ccatcttgat
    16861 tgctgacgcg aatgcggcca acgttctccc tgtcgaggcg gaaggcgccc tcggcctgcg
    16921 ccatgacccg gaacgggcgg ccgaacatgt tgaaatcgtt gacatagacc gatcccatgt
    16981 agatgcgcag ggcctcgaag atgttctcta tcggcacgcc gagcatctgt gccttggtgc
    17041 ggtcgacatc gacgaacacc tgcggcgtca gcgtattgaa cgtggtgaac gtattgacga
    17101 tctccggccg ttcgttcgcc gcgcgaatca gtttctgagc cacgttcgcc agctcgaccg
    17161 agccaatccc tgcgcgatcc tgcaagcgca tcgcaaatcc gccgctcgat ccgatccccc
    17221 gaaccggagg cggcacgatc acgatcgact gcgcctcctg aatttcgtcg ttgagccttc
    17281 gacgcagctc gtcagcgacg cgctcgacgg tgagcccctg cctgctgcgg ttttcgaacg
    17341 tgtcgagcac gacaaatatg gctccgacgc tcgaggatgt cgtgaacgtc gccccggacc
    17401 ggccgaccgc gacattggtg gaagcgacac ccggcgtgtc caacgcgatg gcgttggcac
    17461 gctgcaggac ctggttggtg cgctcgagag aggcgccggg cggaagttgc atggaaatga
    17521 tcgcaaatcc gcgatcgagc ggcgggatga agccggtcgg cgtcctttgg atcatccaca
    17581 gtgttgtgat gatcagagcg gcgtagagca cgagcactga tgcggcgagg cccgccaggc
    17641 gccgcacgag ccggccatat ccccgcgaca gcgcatcgaa gccacggttg aagccgcgaa
    17701 acaaggcccg gataggcatt gagacgatcc ggcccaggcg cgacctggtc ccctctgccg
    17761 tctcatgccg ccggaacatg atggccgcca gtgccgggct gagggtcagc gacacgaagg
    17821 cagagatgat ggttgcgctc gcaatggtga ccgcaaactg gcgatagaac tgccccgtga
    17881 tgccgcccac gaatgcggtc gggatgaaaa cggcagacag cacgatggcg attgcgatga
    17941 gcgcggtgcc gacctcgttc atgctgagcc gcgccgcctc gaccggcggc atgccctcgc
    18001 gcagccggcg ttcgacgttc tcgacgacga cgatcgcgtc gtcgaccacg atgccgaccg
    18061 cgagcaccag cccgaacagc gtcagcatgt tgatcgagaa gccgaaggct tgcattaccg
    18121 cgaacgtgcc gatgagcgac accggaatgg ccaacaccgg tatcagcgag gcccgccagg
    18181 tctgcaggaa cagcagcatg acgaggacga cgagcccgac ggcctcgaag atggacttat
    18241 agagctcctt gatcgagacc tcgatgaact ccgtcgggtt gtagacgatg cggtattcga
    18301 gccccttggg gaagctctgc gacagctcct ccatggtgcg cttgatctcg ttggcggcct
    18361 tgagcgcgtc cgtgcccggc gcctgggtca tgaatagcac agtcgccgga tagtggttca
    18421 tgtagccgaa cgagttgtag ctcagcgcgc cgagctccac gcgggccacg tcgctgagcc
    18481 gcacaattcg gccgtcatcg cccgatttca cgatgatctt ctcgaattcg tccggctcgc
    18541 gcaggcggcc cttgacctgg agaggcatct ggaaagcgcg ctcaccgctg atcggcggct
    18601 cgccgatggc accaccggcc acctgcacgt tctgggcgcg caacgccctc agcacatctt
    18661 cggcgctgag gccgaggctg gcaatgcgct caggctcgag ccagatccgc atggcgtatt
    18721 cgcgcgagcc gtacagcaga accgagccta cgcccggaat gcgggccagc acatcctgaa
    18781 cgttcagcag cgcgtagttg gaaatgtaga cctggtcgta ggtgctgtcc ggtgagatca
    18841 agtggaccgc gaggagctgg tccggattgt tcttgcgtgt gagaatgccg ttgcgcctga
    18901 cctcctccgg cagccgtggc tccgccgcag agacccggtt ctggacctgc acctgagcct
    18961 ggtcgatgtc agtgccgatg tcgaacgtga cggtgaggct catcgccccg tcgctcgtcg
    19021 agttcgagta catgtagagc atgttgtcga cgccgttcac ctcctcttcg atgacggaag
    19081 caacggtatc ggcgacggtc tgcgcgctgg cgccgggata attggtggtg acggttatgg
    19141 tcggcggtat gatttccgga aactgcgtga ctgcgagccc gaagtaggcg atggcgccca
    19201 agagggtgat gaacagcgag atgaccgctg cgaagatcgg gcgggcgatg aagaagtggc
    19261 tgagccgcat ggccgtctgc cgccctggcg tattcctact ggtcgacggg ctttgaaggc
    19321 gccgctgaaa cctgcagcgg ctctttcctg accgctacct tctcctgatg ccgaacgcgc
    19381 tggataccct tgacgatggt ccactcgtcg gcgctcaatc cgctcctcac gacgcgcaga
    19441 ccgtcgatca gcggcccggg ctcgatccat ttgcggatgg ctgtcccgtc atcttcgacc
    19501 acgtagacga acttgctcac ctgatccgtg ccgatcgcct catccgggat catgatggcc
    19561 gaatagctgc ccgagccggc gatctggatg cgcgcaaaca tgcccggccg gaacaggccg
    19621 gacttgttgt cgagcacggc ccgcatgcgg agcgtgccag tcccggtgtc gaggcgattg
    19681 tcggtgaagt cgagcctcgc ctggtgtggg tagccggtct cgtcagggag gccgacacgg
    19741 accgcggtgc cctgtccaac ttggtgaagt ctcaagtact tcagcgcctt gttctctccg
    19801 atatcaaaat agacgtagac cggatcctgg cgaacgatgg tggtcagcac gggtgagttt
    19861 tccgagccgc caaccgccac gtagttgccg gcggtcagct cgctgcggct ggtgcggccg
    19921 gtgatcggcg ccgtgatacg ggtgaatgac agatcgagct ctgctgcctt gacccgcgct
    19981 tcggccacag caacggccgc ctgcgcctcc cgaaggagat tgtcgcgttc gtcgaattgc
    20041 cgctgcgaca gccacccctt ttccaccaga ggccggccac gctcgacgtc cttggctgcg
    20101 ttttccgctt tcacacgagc ttcatgcagc tcagcccggg ccgtttccag cacgcgctcg
    20161 aacgggcgcg ggtcgatggt gtagagcagg tctccttcgg aaacgagttg gccatcggtg
    20221 aagtggacct tctccaggta gccggatacg cgcgcccgaa cctcgaccgt ctcgacggcc
    20281 tcgatgcgac cggtgtactc gtcccagtcg gttacctccc gaaccacggg ccggctggcc
    20341 gtgacggtcg gaacaggcgc ttgcttctgg tctgacctgc ggggctcggt accggtgggc
    20401 gtagaggcag aaaaaaggag tcgcttagcc cagtcgagct ggcgctcagc ccagctttga
    20461 tcggcagcat catcagatgg cgcgctggac gaagccggcg gctcctgggc ccaggcggcc
    20521 gacggaactg acagaacggt ggcgataaaa gcaagcgcga gcgcactgcc aatctgaaat
    20581 ctgccggcaa ccttcaccga cgtgccaagc acacaccgcc ctcgcctgct acgcatacat
    20641 gctcctgggg tgaagccgag gtcttcaccc caagatgaaa cgcaattcta gcaagtcttc
    20701 agcggcttgt aacggcacct cttcgggatc atcctgcgta ttttcttgcg aaaatacatg
    20761 gataagccat cgaaagcctc accatgtcca ccaggacggc gtgccgtatt catagttcgc
    20821 atagcccctc ggcacgcgct ggcgaacgat acgacggcgc ggagtcgatg acctgcccct
    20881 tgccactctc gcgggggctg acctggtctg gcccttgacg atgatctggg tggactgcat
    20941 gccgtgctct tcgacgagct tgtagaacgt agcagcgttc ttggtcgaca ggcggatgca
    21001 gccatgggag gcagggcgcc cgagcaggcc ggtcgcgtag gtcccgtgta cggcgtagcc
    21061 ccgatggaag aagaccgagt agggcatggg cgcgccatcg tactgcctgg agcgccacat
    21121 ccgcgagcgc cactgcgggt agtaaatccc attaggcgta tgatagcccc tgcggccaga
    21181 agaaatcggc cattgatgca gcaccctatc gtcgccggtc atgacggtca tccgctgact
    21241 gctgagatcg atgacggcct tcagcgtcac ttcgggcttc atgaagttgc gctcggggag
    21301 cggcatcagc gcggctgcgg gcatgacggg cgctgcgggc tcggcggcct catcgttgcc
    21361 cgcaactgga ggttgagttg acccgttccc ttcctcgaag atgtcccctg ggctgacagc
    21421 ggtggggaca acagctccgc cggctggact gcttgctggc agctccgcgg tggaaatcgc
    21481 cgtcttcggc ctcggcggct caatgggagg ttgagaggag tcggacgggg ccgtagcaaa
    21541 cgccggccga accatcagaa gtatgccggt gagtacgagc caccccgcat gacgcaactt
    21601 catcgcaact tcctccacgc tcatttcacg caactggcac ggcaggggac aaatcaaacg
    21661 tctaccactg acgatcggca tccatggata gaactgctgg aatatgcaca atcaggtggc
    21721 ccatcgactt cggacgacct ccgtgctcat gccccccact ggctgcgcaa cgcaagaggt
    21781 cggaaatggg atccatcctt caccagacta atttcaaaga tagtgtgaat atcgggccac
    21841 aggtcgcccg ccctcgtact cagccttgca agaacaggca ctctgcccag tgcagcgatc
    21901 gaattgacaa ccgttcccag gtgggcagac ttcctgaaaa tcctatttct ttcaggaggt
    21961 aatcgccgtg accgcagaag cccggaagcg caatatcagc ctcggcgccg ccgtggccga
    22021 tgtggaagcg cgttacattg cagccaatcc gaggagcaag gcacgcttcg aggcggcggc
    22081 ccgcaccatg ccaggcggca acacacggac cattctgcat tacgatccct tccctctcgg
    22141 aattgtgaag ggagagggag ctcgcctgac ggatctggat gggcacgtgt acgtcgattt
    22201 ccttggcgag tacacggcag gcctttacgg ccattccgag ccggccatcg ccgaagcgat
    22261 ccgtgaggcg ctctcgggcg gcctcgttct cggcgggccc aatgcctacg aggcacggct
    22321 ggccgagctc atgtgcgctc gctttccggc ggtcgacttg gttcgcttct gcaattccgg
    22381 aactgaggcc aacctgatgt gcctcagcct ggcgcgcgcc gccaccggcc gggatgccgt
    22441 catggtcttc aatggcgcct accacggcgg cttcctgtcg ttcaccaagg gtcactcccg
    22501 cgtgaacgcg ccgttcgact acgtgatggg cgagtacaat gacccagaag gcgtccgtcg
    22561 cctgatcggc gagcacgcca gcaggctcgc cgcggtcatc atcgagccga tgatgggcgc
    22621 cggcggctgc atcgctgccg accccgagtt cctgtccatg ctgcgggagg agacccggcg
    22681 gcacggaatc gtgttcatcc tcgatgaagt gatgacctcg cgactggcac ccggcgggct
    22741 gcatggcgcg ctcgggctga caccggacct cgtctcgttc gggaaatatc tcggcggcgg
    22801 agttaccttc ggagccttcg gcggcagccg tgagctgatg ggtctgcttg accccacacg
    22861 tcccgacgga ttcatgcatt ctggcactta caacaacaac gtgctgacga tggcagccgg
    22921 cgtcgcggga ttggagaagg tcttcacccc ggaaagggcg gaagcgctca atgcctccgg
    22981 cgatcgcctg cgtgagcgcc tcagcgcagc aatcgccaag cacgacgctc ccctgcaagt
    23041 tctgggccgc ggctcgatgc tctgtatcca tccgcaaagg gcacccatcc gtcgccctgc
    23101 cgaccacgcc gcggcgtcgg ccgacgcgcg caagctgctg cacctcgacc tcaaattgcg
    23161 cggcctctac accgcgccgc gcggtttcat gagcctgtcc ctgccactga ccgaagccga
    23221 ccacgacgcg ctcgtcgccg cgttcgaggg gtggctggag gactatgcgg gtgcgctggc
    23281 gcaactcgac tgagcaagtg ccgcgttttc tctcgagaga ctaccctttc cggcgcacga
    23341 tcatctcgcg ccggaacgta tagatgccgg cacaggttac aatcgccatt ccgagcaggg
    23401 tcagcgtgtc gggcagatcg ccccagatca ggaggccgag gatcagcgcc cagatcagaa
    23461 tcgagtagcg gaagggggcg acagccgaca gctcgccctt gcgcacagcc gagatgatgc
    23521 agatatgccc cgtgaacgag aaggcgcttg ctccggccac ttgcagcagc aggcccggcc
    23581 ccggcaacac ccagtcctcg aaaaacacca gaaacagcga accgatcgcc accacgcctg
    23641 atgagtaaag catgatcagg gcggccggaa ccgcccggtc gatgcttttc gtcgccacgt
    23701 cgcgcaaggt cgcaaacagc accgaaacga tggcgagaat gctccaccac gtaaaagcgc
    23761 tcgttcccgg gcggatgatg agcatgacgc cggcgagccc gacaacggca gccgtccatc
    23821 gccgccagcc gacgcgctcg ttgaggaaca ccgccgaggc cgccgtcacc gtcagggggt
    23881 tgagctgaat gatggcgacg agatcggcga aggccatgcg cgccagtgcc gactggaacg
    23941 ccagtgcgcc gccgacatcg ccgatcgcgc gcagcagcat cggccgcgat agaagcttcc
    24001 aggcgttgcg gagggctccg gagaaggcgg cgtaggtaaa cgccgcgccg accatgcccg
    24061 tcgcgcgcaa aaaaacgagc tgtccggtcg gcatctcggt cgaggaaagc ttcatcagcg
    24121 agtcgccgca ggcgaagctc gccattgcgg cgagcatcgc gatgatgcca gccgtattgt
    24181 tcgtctgtgc gagatttgcg gccgcaacat tactggccgg actgccgccg gggcccggtc
    24241 tgccatctcc gaccaccgca cactccttga tacccggcaa accgggcgcg actctcaacc
    24301 cgtcagatta gcgcaatcgt gccccgcggt ctcggtctgc cggatcggaa tcgatgtcct
    24361 caccgtcgtc cacgtcagag gaaaaaagcc ccatttgcgg ggacgacgaa cgcgcaggtc
    24421 cggcgagccc caggtggcga taggccttga gcgtcacgat ccgcccgcgc ggcgtgcggc
    24481 cgatgaagcc ctgctgcagc aggtacggct cgacgatctc ctcgagcgcg tcgcgcggct
    24541 cggacaaggc cgccgcgatc gtctcgatac cgaccggacc gccttcgtag ttggtcgcga
    24601 tgcacatgag atagcggtga tcgagcgcat cgagacccgc ggtgtccacc tccagcgcgc
    24661 gcagcgcctt gtccgcaatg tcggcgtcga tcacctttgc tcccgccacg gctgcgaagt
    24721 cccgcacacg gcggagcagc cgtccagcaa tgcgcggtgt tccccgcgaa cggcgcgcga
    24781 tctcgcgagc tccttccgga ctgatcgcgg cacccagcac ccgggcaccg cggctgacga
    24841 caagctcgag ctcgtccaca gaatagaagt tgagccggat cgggatgccg aagcgatcgc
    24901 gcaggggggt cgtcagcagg ccggtgcggg tcgtggcgcc gaccagggtg aacttggaga
    24961 gatcgatccg caccgagcgt gccgcaggcc cctcgccgat gatcaggtcg agctgaaagt
    25021 cctccatcgc cggataaagg atctcctcga ctgctggatt gaggcggtgg atctcgtcga
    25081 tgaagaggac gtcgcgctcc tccagattgg tgagcagcgc cgcaaggtcg cccgccttgg
    25141 aaatgaccgg gccggaggtg gcgcgaaacc cgacccccag ctccttggcc atgatctggg
    25201 cgagcgtcgt cttgccgagc cccggcggac cggcgaacag gacgtggtcc aacgcatcgc
    25261 cacgctcccg cgccgcctga atgaagacct tgagattggc ccgcgcctgt tcctggccga
    25321 tgaactcgtc gagcgactgc gggcggatcg tcgcctcgaa tgcatccgtc tcgcgcttag
    25381 cgcctgtgac gaggcggtcc gtcatcgcac ctgtcccccg ctcctgttcg tgattcgtgc
    25441 ccgcctgaac gtctaccaca aatcgccgtc cctcgccctg cggactgttg aacatcgcga
    25501 gcatcattct cgcgaaatcc tgtcggcaac gtcacacgtc gtcgggtagg gtgaggagat
    25561 ctgcgaagct cgcgaacagg gaggacgcca gcagatgaat tggacccgaa cgaggcgggc
    25621 gatagcgctc gccgtcctga ccgtcgccca acctgcggga tatttggccg gcgcagcgtg
    25681 ggcgcaggag cccgcactcg atccggtcca cggccgcgac gtcgcccagc gcgtttgcgt
    25741 cggctgtcac gcgatcgagc ccggagtgca acagaggcag gcggacgtcc cctccttccc
    25801 cgagatcgcc aaccggccgg gccgcacgat ccaaacgatc atcggcgcaa tgtactatcc
    25861 gcacccggag atgccgggcg tacctctgac caccaaggag acgcgcgacg tcgccgccta
    25921 catcctgact ttccgcaagg ctcagtgagc cggcaaaggc tccaggacgc cgatcctgtc
    25981 gaggatggtc cggcagatga cggacacgac gccggcgagg aaggcgaggt aagggatcgc
    26041 ctcgggcacg ggcaacgcga acagaaccgc cgtgaccgcg aaggcgatca cgcccgcgat
    26101 ggccgcagcg atccagccga acgtcgcatt gcgaagaaca agaggtagaa ggccgagcgc
    26161 ggcaagggcg gaagccggcg cggcccagac ataagtgaaa aaggcggccc atccgacaaa
    26221 ggcgacctca ccggcggctt cgaggttggc aatctctggc agcaagccgg ccaccaatgc
    26281 caagggcatc agaacgctga gcagaagcgc gccgatgaac ggcccgacaa gggtgtagaa
    26341 cagaaacgtc cacagcgcgc gctgggcctt cgtcatgcct tccatcggat ccgacccgtt
    26401 atctcgagag ctccttgagg ccccgcttga tgagctgggc gatgtccgcc gtgttgccga
    26461 gctcgcccat ggccgatgcg atcgcggctg cggcctgctg cggcgaatac cccagatttg
    26521 tcagagcgga aatggcttcc gcagaggtgc tgcgatcttg ctgcgacgcc ggcccggcga
    26581 gcccattgac ccgcagtccg gccggcacgc tgacgctcgc cacggtgagt tgcggcgcct
    26641 tctccttgag ctccgcgacc acgcgctgtg cgagcttctt gccgacccct ggcgactgct
    26701 ccacggccgc ccagttcgaa agcgcgatgg cgttggcgag gtcctgcgtg gacaaggtcc
    26761 cgaggatggc aagcgccacc ttcgagccga ccccctgcac cgactgcagc gcccggaacc
    26821 aggccctctc gacctcgctg gtgaacccga acagtcggat cgcgtcctcg cggacgtgcg
    26881 tctcgatcga cagtgtgacc tcatcgccga gggtgaggtt tcgcagcgtc cgtgccgagg
    26941 cctgcacctc gtagccgacg ccgttcacat cgatgatcaa gtagctctcg ccgatggcgt
    27001 ccaccttgcc ccgaagacga ccgatcataa tgggggcctc cctcagccgc cgaactgccg
    27061 tcccgcgcgc gccgagacga cttgccgtgc cgcccgatga tgcgcatgac aaatggcgat
    27121 ggccagggca tccgcctcgt cggcgttatc gaacttcgcc ttgggcagca gaaagccgat
    27181 catcgcctgg atctggcgct tttccccgtg cccgttgccc gtgatggatt tcttgatcag
    27241 gttcggcgga tattcggcca cgcgcagccc tttcatcgcg ggggccagca gtgccattcc
    27301 acgcgcctgg ccgagcttga gcgttgcgcg cgcgttctcg ttgacgaagg tctcctcgac
    27361 ggcagcctcc tgcggggaaa agctggcgat cacgctcgac agcccctcga agatctcgcg
    27421 caggcgatag gccaggtcat cttcgcgatc ggacgcgatc tggcccgacg ccacatagcc
    27481 aagccgcacc ccgtccgact cgatgacgcc ccagcccgtc cgccggagcc ccgggtctat
    27541 gccgatgatc cgaatcgatt tcccgcgcat ggggtgtcag tagcacagat cggactgcgc
    27601 tgccccgatg cgagcaggag aaaggtcgcg cgagaggtcc agccaccccc ctcaagccgc
    27661 cgtgagacgg gccatcacct cctcgtccac ctcgaagttc gagaagacgt tctgcacgtc
    27721 gtcgtcgtcc tcgagggcgg agatgagctt catgatggtc tgggcgttgt cctcgccgac
    27781 ctgcgtggtg acgttcggtc gccagatcgc tttcaccgag gtggcttggc cgaacttctc
    27841 ctcgagcgcg ttggcaaccg agccaaggtc ctcgaaggcg cacgttacga catggccgtt
    27901 ctcgtcggat tggacgtcct cggcgccagc ttcgatggcc gcttccagca tcgcgtcggc
    27961 agatgcggcg cccggcgcgt agaccacctc gccgatgcgg tcgaacatgt gcgcaaccga
    28021 gccggtcgag cccaggttgc cgccgtattt cgtgaacgcc gcccgcactg ccccgcctgt
    28081 gcggttgcgg ttgtccgtca aggcctcgac gatgatcgcc acgccgcccg gcgcgtagcc
    28141 ctcatatctc actgactcga aattctcgct gtcgttgccc tgggccttct tgatggcgcg
    28201 ctcgatgttg tccttcggca tgttctccgc gcgcgccgcc tgaaccgcga gcctcagccg
    28261 cgggttcatc gccgggtccg gcaggccgga tttcgcggcc accgtgatct cgcgggcgag
    28321 cttcgcgaac agtttcgcgc ggaccgcatc ctggcggccc ttgcggtgca tgatgttctt
    28381 gaactgtgaa tgtcctgcca tcggaatgtt cgtgtcgact gcttgagact gcagcgggcg
    28441 ccagtaactg cgcccgtctg tgaaaagcgt tcgagatgga tctcctccct tatagttcgg
    28501 gagcgctgct ggaatccatc cctcggcgag ccggaggagg cactaacctt ttgttaaccc
    28561 ttgacgcgga gcctcgacgc aggtgattag cacctgttgc cgcgggctgc ccacccgtgg
    28621 ctcgacctga ggcgcgttcg tgagcctctg accgaccgtg agggcgcgat gatccgtcct
    28681 caccggtcca gtacttgcca aatcaatctt cctcgaccca tgacagcggc tcggccggtg
    28741 ccaagacacc accgagccgc aacacgtcta cgcgcaccgc caacccggag gcgtcatcga
    28801 tctcgatggc aacgccggag atggtggcgg gtccgagcgc tggctcgaag cgctcgcggg
    28861 aaatgcgggt gaggaagcgg ttgatgggct cggtgggatc catgccgagc accgagttgt
    28921 aatccccgca catgcccaca tccgacatgt aggccgtacc gcccggcaga accctgtagt
    28981 cagaggtcgg cacgtgcgta tgggtgccga taacgccgct cacccggccg tcgaggaaat
    29041 gagcgagaga ctgcttctcc gacgtcgcct ccgcgtggaa gtcgacgatg atcgcatcgg
    29101 cgccacgttt gaggccgcag gcgttgacct cgtggtcgat cgcgcggaac ggacagtcca
    29161 tctggctcat gaagacgcgg ccgagcgcat tcatcaccag cacctcggcg ccgttggccg
    29221 cgcggaacag cccggagccc ttgcccgggg tgccgggagg caaatttatc gggcgcagca
    29281 gacgggggtg gcgctcgatg aagaccagcg cctccttctg atcgaaggag tgattgccga
    29341 gcgtcaccac atcggcgccg gcaccgatca gctcctccaa gatctgttct gtgatgccga
    29401 agccaccggc ggagttctcg ccgttgatga ccacgaaatc gagcttgtag cggcggcgca
    29461 ggcccggcag ggcatcgacc acggccttgc ggccggatcg gccgacgatg tcaccgagaa
    29521 aaagtagtcg catcagtctc taacacatct gcgcgggccg gtaggggtca acacccaatc
    29581 gagccgctgg tcaaaatcaa gccgtggtac ggccggcact tcttggacat cgtgggcgat
    29641 gccgatagct gtgatctttc cctggctgcg cgctgctgca agtgtacgat catagaaacc
    29701 gcccccgtag cccagacgga accccgccgc gtcgaaagcg agcagcggaa ccagcatgac
    29761 ggccgaaatc acttccggtg cagacggcag cggctcgcga atgccccagg ctttctcctc
    29821 cagagggtcc cctggagtcc ataggcgaaa ccgcaacgga gtagccttgc catccatgac
    29881 cggcaaggcc agctggaagc cctcgcgcgc gagcctgagc atcagcggct gcgggtcgat
    29941 ctcgtctccg acggggaaga accctgacac gacagcgcca gccggtggcg cgacaaaatc
    30001 gatgccgtat gccgcaagct gctcggcggc ccagcgccgt tccctcttgg aaatctctgc
    30061 gcgaatgcgc cgggcggtcc tgcggagctc ctgcttcgcc gcactcactg cggaaggatg
    30121 gaccaccgcg catcgcacct cgctatttgg ttccggccgc gagtgccgct ggggagcggc
    30181 tgggccggag cgaaccagaa gacgaatgcc gcgaaggccg tcggagcgaa tgatcccgcc
    30241 aggtccctac agaagtaggt gggcgccgtg tgcccgagac cacggtcaag ggcagggaca
    30301 gctcccaagc gagtcttatc ggcccagggg aaaacgtgct cctaacgagc cccgcagcag
    30361 atacgaatgt atggaggtcg cgcgcgaatt ccaaccgtga tcttcatagt gcgacggcgc
    30421 ggccgcgacg tggccctaaa agtcaggcaa cccccgcccg tgcagtgcgc gctgaccgca
    30481 tcaggcccaa ctgctctcgg gcctcgaaaa gctcgtcggt gaccaacaag gcggccatca
    30541 gcatcagccg atcatcgccg atctgcccga actgagccac aagcccttcg atcttggatt
    30601 tcacgtgatc cgccaaagag aacaggcgtg gctcctcgcc gtcctcacag cgcaggcggt
    30661 aggtacgccc gttcagcgtc accgaaacct gccccatccc cccatcggca acccgcgcat
    30721 ggctgccgcc tcatgcgaat cagttttgac gctcaatgac gttatgaatc gaatcaataa
    30781 cccaatcaat acgattaagc acttcgagcc gctggctttc aagcagcttg atccgagcct
    30841 cggcaacatc aagtcgcgtg cgcaattcat cccgctcgcg caacagttgc tcgacctcgc
    30901 tcgcgcgtga tttggaagct gctggcgtgc ttgtgcgcgc cccccggctg cttgctcgtg
    30961 gcgcagccgt tctcctcttt ttttgccgat ccctctgcgt cattcgacgt cgtcctggcg
    31021 tgcgacgcag agcccccctc tggccatgag catcgccgca tagaccttaa gtcgcgataa
    31081 gctggcgcgt caactcgacc gacgtcgtac aattcacact cctatacaac tgctgcatcc
    31141 gggccgcaca cattgacatg gcggcaggcg cggaacatgt aaacgcggga taaatttagg
    31201 ccagcaggca gttttcattg cctgagggtt ggcaaccgct cgccaggaga aagcagatga
    31261 caatcaagat cgcgatttcg ggtttcgggc gcatcggccg caacgtgctg cgcgccatcg
    31321 ccgagcagaa gcgctccgag gttgaggtgg tcgcgatcaa cgacctgggg tcgcctgagg
    31381 ataatgccca cttgttcaag tacgacagcg tgcacggcac ctaccagggc gaggtgaagg
    31441 tcgatggcga cctgatggac atcggctacg gcaagccgat caaggtcctg gcgatcaagg
    31501 accctgccca gctcccctgg aaggacctcg gcatcgacat cgtcatggaa tgcacgggca
    31561 tcttcaccga caaggagaag gccaaggcgc acctcgaggc gggcgctaag cgcgtgctcg
    31621 tctcggcccc ctcgaaaggc gccgaccgga cggtcgtcta tggggtcaac cacgacaagc
    31681 tcacggccga tgacaaggtc gtctcgaacg cctcctgcac cacgaactgc ctcgcgccgg
    31741 tggccaaggt tttgcacgag ctgtgcggga tcaagtcggg cttcatgacc acggttcacg
    31801 cctacaccaa cgaccagagg atccttgatc aggcccacaa ggatcgccgc cgggcgcgcg
    31861 ccgcggccat gtccatgatc ccgacctcca cgggcgccgc cgcggccgtc ggactcgtgc
    31921 tgccggagct caagggcaag ctcgacggga ccgcgatccg cgtgccgacc cccaacgtgt
    31981 ctgtggtcga cctgaaggtc atccccgagc gcgagacgac cgtggaggag atcaaccagg
    32041 caatgaagaa ggcggcctcg caggaattga agggcgtgct cgccatcaac gacaaggagc
    32101 tggtctccat cgacttcctg cactcgcctg cctcctcgac cttcgatgtc acgcaaacgc
    32161 agatcgtcga cggcaagctc gtccgcgtgc tctcctggta cgataacgaa tggggctttt
    32221 cgaaccggat ggtcgacacg gccgtcgcga tgggcaagct gctctgaggg aaaagtcatg
    32281 tgtgcaagca ggagcgccgc tggtcctttc gtgaacgagt ccacaggacc cggcgctggg
    32341 gcatgccctg ctcgctccgg ttccatttgc tttcgcaagc gctcgaggaa tcgagcgctt
    32401 gttttctgcc gaaggccgct cccgctcgtt ggcgaacggc cggccttctt gaggtgttga
    32461 tggtcgagtt gtccccgagg agacctgact catgactgaa cgactggaag atatcgccca
    32521 agccatggtc acccccggca agggcatact ggccgccgac gaaagcaccg gcacgatcaa
    32581 gaagcgcttc gactcgatcg gggtcgagaa caacgaaacc aaccgccgcg actatcgcga
    32641 gatgctgttc cgcgccaccg aggcgatgcg ggactatgtc tccggcgtga tcctcttcga
    32701 cgagacgatc cggcaaaagg cgaaggatgg cacgccgctc gtcgacctca tcaaggcggc
    32761 cgggtcgatc cccggcatca aggtggacgc cggcgccaag cctttggccg gcccgacctc
    32821 ccgcatcgag aaggtcacgg aaggtctcga cggccttcgt gagcggctcg ccgagtacta
    32881 caagctcggt gcccgtttcg cgaagtggcg cgcggttatc accatcgccg acggctgccc
    32941 gacctggaac tgcgtgcatg ccaacgcgca tgcgctcgcg cgctatgccg cgctgtgcca
    33001 ggaagccaac atcgtgccga tcgtcgagcc tgaggtgctg atggacggcg agcattcttc
    33061 acacgacatc gaccgctgct atgaggtgac ggagtggacg ctgaagaccg tgttccatga
    33121 gctctatgtc gccaacgtca atctggaagg catggtgctc aagccgaaca tggtgatcgc
    33181 cggtcagaag tcgccgaagc aggcaagccc gcaggaggtt gcagagcgca cgatcaagtg
    33241 cctcaaggcc tgcgtgccgg ccgccgttcc cggcatcgcc ttcctttcgg gcggccagtc
    33301 ggacgagcag gcaacggagc acctgtcgct gatgaacgcg atgggcaatc tgccctggaa
    33361 gctgacgttc tcctacggcc gcgccctgca ggctgccgct ctgaaagcct ggggcggcaa
    33421 gccggaaaac gtcgccgccg gccagcgggc gttcgcgcat cgtgccaaga tgaacgggct
    33481 ggcctcgctc ggccgctggg agcgctcgct ggagcaggcg gcgtaagtgt tcgctcggcc
    33541 gctgatctac aggaccggag ccgatcggct ccggttttgt gttcggtggt cggccagtcg
    33601 cggcagcacc cgactccact gctatcttac tggtaccgat tgcgaacgac ccaaggcatc
    33661 ggataagcgc gttccagtcg agcggtgtca ccgtttggcc ggatttgcgg ccgatagcgc
    33721 tgccgctgga gctgtttcga ttgaggtgga aacgccaaca gcaggcactc cattaacctc
    33781 tgccacaggg agaggtcggc ccgaaaaccg ggtgagggat ggcaacagcc gccaccggcg
    33841 aaacgccttc accccgaccc gctccccact ggggagattg ggcgcgagct tgacttggcg
    33901 tgcttctgtc taatcggaaa tgctctcgtg cgaagaatgc ctggagcagc cggatcatgg
    33961 aacaggaagc aggctgtcag ctcttcctgg tcgtggaggc cggggagtcg gccccggatc
    34021 gtctggcggc cgcgctggaa gttgtgccga ttgcttccgt cctcatcgtc cctcccgccg
    34081 gccagtgcct cgctgaagca tccgccaagc cgctcgtgga gcaagctcag gcccaagggg
    34141 ccgccgctct ggtcctggac gacgttgatc ttgcccggaa actgcgtgcc gacggcgttc
    34201 acctccagca cagcaaggac atcgaaagcc gctaccgcgc tgcgcgccaa gcccttggcc
    34261 gcggtgcgat catcggcgtt catgccggca agtcccgtca tgatgccatg gtcgtcgccg
    34321 aggagggcgc cgaatacgtc gcattcggtc ttcccgaggg ggtgacagac agtgcatccg
    34381 cacgggagcg ccggttggat ctcgtcgcct ggtgggccga aatcttcgag gtgccctgcg
    34441 tcgcactcga cgtcgcgaag ccggaggatg cggcagagct ggcgcgggcc ggtgccgact
    34501 tcatcgggtt gacccttcgc gctggcgaac cgctgtccaa catcgagcaa cggatgcgag
    34561 cgatggctga ggcggcccaa tcggcgctgg cggagtaaag gggcggaccg catgcgaacc
    34621 gcgacgaagg tgttggccgg ctggctcggg ctgctgatcg cgtttcctgc ggcgtttgcc
    34681 gaaacgagct cctggtcgac cgaaaccgcg cccagtgttc ccaaggcgaa ggcacaggcc
    34741 aagccgaaac cacaatcgaa gccgatggcg cgcccgcagc cgaagggccc caagccgctg
    34801 cagaacaatc aggccgccga ccccctcagc aggttcacgc cgggcaaact cgaaagctcg
    34861 ttgtcacgct accttgtccc ggcgacgggc gaggacgccg catacatcgc cttcgagcag
    34921 ggccaatacc tcacagccct caaccttgca gagcgcgcgg ccaatgaggg tgacccccag
    34981 gcccacacgc tggtcgggcg catctacgcg gaaggcttgg gagtgcctca gaatgaagcc
    35041 gttgcagccc aatggtacgc tcggggtgcc gagctcggcg acattgagtc ggccttcgcc
    35101 ctcggcctgc tgttcgccga aggcaagggc gtggagaagg atcgggcagc agccgcgcgc
    35161 atgttcgaga tggccgccgc caagggtcac ccgtatgcca attacaacct cggcgtcctg
    35221 ttcctgaacg gcgacgggaa acccgagaac ccgacccgcg gcgccttaca tattcgatac
    35281 gctgcggagc agggcgtggc cgccgcgcag tacgacctcg ccgtcctcta ccagaagggt
    35341 tacggcgtgg agcccgacgc ttatcaggcg gctctctgga tgaagcgtgc cgcggatcag
    35401 ggcctggtcg cagccgaata tgactacgcc gtcatgctgc tgcgtggctt cggtttcaac
    35461 gcggatgtgc caaaggccgt cgactatctg cggtccgccg ccgagaaggg ctttgccgga
    35521 gcgcagaacc gtctcgcgca tgttctggcg gacgacagcc acggcccgcc gaatctgttg
    35581 gaagcagcca agtggcgcat ccttgcgcgc gacagcgggc tcgaggacaa ggagctcgac
    35641 gagctcgtgg ccaacctctc ggagtcggac cggctggcgg ccgaaaaggc agcccaggag
    35701 tggcgggaac gggcggcgat cggcgtcctc acgaactgag ccaaggctgg ctcgcccttc
    35761 gcggcagggt cctccccttg tcattcggag ctggagccgc tagtcgaacg gccaactctt
    35821 aaaggcccgc acgaggtgca ggtctatttt gtcttgctca gccgtgtcga ggcgctgttc
    35881 acatgccagc atctgctctg atgaacgtca tgatcgccgc tgcgcgcaag gccgcgcgca
    35941 gcctggcgcg cgacttcggc gaggttgaac aactgcaggt gtccctcaaa ggtccggcga
    36001 atttcgtttc ggcggccgac cgacgcgcag aggaaatcct cttcagggag ctgtcgcgcg
    36061 cgcggccagg ctacagcttc ctgatggagg agagcggcga gatcgagggc gcggacaaga
    36121 gccatcgctg gatcgtcgat ccgctcgacg gcaccaccaa cttcctgcac agcattccgc
    36181 tgttcgcgat ctcgatcggg ctggagcggg agggtcagct cgttgcagga ttgatctata
    36241 atcctatctc agatgagatg tatacggcag aaaagggcaa gggtgcattc ctgaacgacc
    36301 gtcggatccg agtagctgca cgccagactc tttcggatgc ggtggttgcc accggcattc
    36361 ctcaccgcgg ccggacagga cacctgcgct ttttgcggga ggcggaagca ctcatgcggg
    36421 aggttgccgg gatccgcaga acgggttcgg cggcgctcga tctggcgtgg acggcagcgg
    36481 ggcgcttcga cggctactgg gagcatggcc tgcaggcgtg ggacctggca gccggcgtac
    36541 tgatcgtgcg agaggctggc ggattggtta ccgatacgga gggcggcagc cgcattttcg
    36601 agaacggcga tgttcttgca ggcaatcctg caatctcgag agcgttgata tcggtcctca
    36661 acgcggccaa gcgggctgca tgatggcgct tgcaggccgc catcgggcac gatattgccg
    36721 gttttccctg ccaccagatt ttcgctaagg tcgctgtcgc gtccgtcatg aggctcaaat
    36781 aatggccaga aagcgacaag ctgaaatctc ggcaaaagcc gtccaccgca ccttgagttc
    36841 gccgggcggc tttctgctca atatgatttt cttcctgacg atcgtcggct tcattgccgc
    36901 gatcgccatg ccgatgcttg ttacggcgtt cctcaacaat cccttcctca acagtctgat
    36961 catcttcgtt ctgttcatcg gcatcctcta ttcgttccgg caggtgatcc gtctctaccc
    37021 ggaaattcgc tgggtgaacg cctttcgcat cgccgacccg ggactcgcca tttcgcacag
    37081 gcccgtcctg ctggcgccca tggccaccat gctgcgcgat cggacgggta cgctgtcgct
    37141 gtcgaccgtg tcgatgcgct cgatcatgga ctcgatcggc tcgcgcctcg acgaggcccg
    37201 cgataccgga cgctatatgg tcgggctgct cgtgttcctc ggcctgctcg gtacgttctg
    37261 gggtctgctc gagacgatta cttccgtggg taccaccatc agcgcgctcg ataccagcgc
    37321 aagcgagagc tcgatggttt tcgaggagct gaaaaccggc ttggcagcgc ctctgaaagg
    37381 catgggcacg gcgttctcat catcgctgct gggccttgcg ggatcgctgg tgctggggtt
    37441 cctggagctg caggcgagcc acgcgcagaa ccgtttctac aacgagcttg aggagtggct
    37501 gtcgggcatc accgagctca cgccgggtgg ctcgggcgcg gaccagctga accgccagct
    37561 ctattcggcc gtgttcgaga tgcagcgctc gatcaccgag ctcaccgacc gtcttcaggc
    37621 ggcattgcca ctggatgcca acggcagcgg caacgacgcc gtgcgtgatc tggcgcaagg
    37681 ggtcaacgag ctggtggcac agatgcgtgc cgaacagaac gtcgtgcgcc agtgggtgga
    37741 cgagcaggcc gcccagcaga accaagtaac gggcatgctt cgtgatctgg cgatgaactt
    37801 caagcggcgc ggggcgtaag tcatggcgcg cgggcgcagc cggcacgggg gtcagtacgg
    37861 cgaattctgg ccgggttacg tcgacgtgtt gtcgaccctc ctgatggtct tcaccttcct
    37921 gctctcgatc ttcatgctgg cgcagtactt cgtgagccag gaagcgagcg gcaaggatgc
    37981 ggccctgcgg cggctgacgc gccagatcgc ggagctcacc aacctgctct cgctcgaaaa
    38041 aggcaaggcg aagactgtcg aggacgatct cgccgccctg caggcgacgc tcgcaacctt
    38101 acgcgacgaa aacgcccggc tgtcaggtct cgtcgccgag cgcgacgagc agctgaggag
    38161 cggccagggg cgcatcagca cgctgaccgc tagtctcgac gagcagacca agatctccaa
    38221 cgaggcgctg gccaaggtcg acctgctcaa tcagcagctc ctcgcgttgc gccgccagat
    38281 cgcggccctc aacgaggccc tcgaggcctc cgagcgcaag gatcaggagg cgcaggatcg
    38341 gatcaaggat ttgggccagc ggctcaacgc ggcacttgcg cgccaggtac aggagctgca
    38401 gcgctatcgt tcggacttct tcggccggct gcgtgagctg ctgaaggacc ggaaggatat
    38461 ccgcatcgtc ggcgaccggt tcgtgttcca gtccgaggtc ctctttccgt ccggtcaagc
    38521 tgagatgacg cccgagggtc ttgccgccat cgaccaggtg gccacggcga tcctggaact
    38581 cgagcgttcg atcccaagcg agatcgattg ggctctgcag gtggatggcc acaccgacag
    38641 ccggccgatc agcagcttcc aattcccttc caactgggag ctctccaccg cccgcgccat
    38701 ctccgtggtc cgttatctca tctcgcgcgg cgtctcgccc aagcgcctca tcgcggcggg
    38761 ccatggtgag ttccagccgc tggaggaggg cgacagcgaa gaagcccttc gccgcaatcg
    38821 ccgcatcgag ctgaggctga cgaacagata gctactgcgc cgcctcccct gcgaactgca
    38881 gctcggccag tcgggcgtac aagccaccct tgcgcactag cgtcgcgtgc gtcccttcct
    38941 cgaccaagcg gccctcgtcg agcaccagga tgcggtcggc cttctggatc gtggctagcc
    39001 ggtgggcgat gaccagtgtc gtgcggccct gcatcacctg ctcgagcgcc ttctggaccg
    39061 agacctcgct ttcggcatcg agcgcgctgg tggcttcgtc gagaagcagg atgggagcat
    39121 cgcgcaagat ggcgcgggcg atcgcgatgc gctggcgctg tccacccgag agcaacacgc
    39181 cgcgctcgcc aagaagcgtg tcatagccct gaggcagcgc cgaaatgaac tcatgggcct
    39241 gcgccagctt tgctgcacgc accactgcat ccatgcctgc gtccgggctg ccatagctta
    39301 tgttcccggc aacggtgtcc gcgaagagcg caacgtcctg cggcaccagc gccatccgcg
    39361 cgcgcagttg ctccaggtcc gcttcagcaa tcggaacacc gtcgacgcgg atcacgcccg
    39421 cggtcggatc gaagaagcgc agcagcaggt ggaagatcgt gcttttgccg gcgcccgacg
    39481 gcccgacgag ggctaccgtc tcgccggctc caacctcgaa cgtcacgtcc tccagcgccg
    39541 attcatcgcg cgacgggtaa cggaagctga cgtggtgaaa tgcgacctca ccccggccgg
    39601 ggaccggcag cgggatggga ttgtccggcg tgcggatctg cggctcgatg gcgagcaact
    39661 cggccaagcg ctcggccgcg cctgctgcct gggtgacctc gccccagacc tctgaaagct
    39721 gggccagcgc ggccgccgcg aacacggcgt aaagcacgaa ctggctcaat cgcccaccgg
    39781 tgatatagcc tttgacgacg gcatcggcgc cgaaccagag aacgccgacg acgctggcga
    39841 agacgaggaa catggtcatc gccgtgaggc cggcccgcgc cttgagccgc gccctcgccg
    39901 cctcgaacgc cgtctcgacc gctcccgcaa acttcgccga tacggtccgc tcgctggtga
    39961 aggcctgcat cgtgcgcacg gcgccgaggt tctcggtcgc gagggccgag gcctccgcca
    40021 gtgtatcctg agccttccgc gacagccgcc gcaccagccg cccataggcg atgagcggca
    40081 gcaggatcac cggtatggca accaacacca ggaacgacag ctgcgcgctc gtcacgaaca
    40141 tcatcgccag cgcaccgacc agcatgatgg cattgcgcag cgcctggctg atcgctgtgc
    40201 ccgctgccgc cttgatctgc gtcgtatccg ccgtcaggcg gctcgtcacc tcgccggagt
    40261 gggtggtgtc gtagaactcc gggccgagcc gcgtcagatg ccggaacacg tccgtacgca
    40321 ggtcggcgac caccctctcg ccgagccagt tgacgaagta gaaccgggcc gcgctcgcca
    40381 cagcgagcac gaacccgatg aacatcagca tcgagaagta gcgatggatg aagaggccat
    40441 cggctccacc aaagccgtag tcgatcacac ggcgtacggc gatgggcaga accagcgtgg
    40501 cgagagccgc catcaacagc gcgatggcgg cgagcaccag catcccggga tgcttgaaga
    40561 cgtagggctt gagcttcagc aggggtcgca gcgaccggcg gcgggcctcg gcggtgtcct
    40621 cacctggctc tgccagctca gccttgctcc ttcttggcct tctcacaact cgtaccccgc
    40681 tggtcggctg aatcgtgact ttgctgatcc gtgttaccgg tcgccacaga tatgccagat
    40741 tacggagccg gttcgggctg cagcgatgcc gcacatgccg ggacggcttg tgtcaggcgg
    40801 cgctttcaac tatgtaggcc cctcgacgca tcccccaatc gatcgaccgt gtccgctctc
    40861 ggcggagagc gggacctttg ttgctgagag ccgccatgaa gagagatcct gaccttcatc
    40921 ccgattacca ccagatcaag gtggtcatga ccgatgggac cgagttcacc acctactcga
    40981 cctacggcaa ggaaggcgac acgctgacgc tcgacatcga cccgaagacg catcccgcgt
    41041 ggactggtgg cgatcagaag ctggtcgacc gcggcggccg actgtcgcgc ttcaagaaga
    41101 agttcgaagg cctgttctga gcctcgaagg cccccgccgc cggagcagcc ctccggcgcg
    41161 gattggccgc aaccggtggg ctgcggctca tgaggctgtg cccgcttgta ttgggcgtcc
    41221 cgtagtggcg cgatgccgac gggagcctcc tcagcgaatg gcggcgttga aggcggcttg
    41281 cagctccgcg atctgcgcgg ccaccgggtt cttggagggg cgcggagccg catcgccggt
    41341 tgcggttacc tgctccaacg agcggtccaa acgtatgatg cgatcgctca acgcgaagct
    41401 ttcgttgata agctgctgca gctgctccgg cagctcgttg aaatttggga tgtgactcgg
    41461 ccgacctgac gtgcgcagtt tcaggcgtgc gcgcttgcgg cgggcctcct cctgcgtgat
    41521 gtcgccatcc cgcaatgagc gccggatcaa aagccaagac gccagatcga gcaggcgcgc
    41581 ggtgaggcgc atgctttccg tggagtacgt cagcgccaca ttcggcttga gctgctttgc
    41641 ttcctggcgg ccggcaccgt cgagataggc tgcagtgcgc tcgaccagac ccatgccctg
    41701 cgcatagacg gcctcgaact gctcggacgc cgcgaagcgc tcaccgaacg agatggtgac
    41761 acaggcgctg ggctcgtccc tcagactcga tttgctaccc atgacctact cacgccacaa
    41821 ctgaaccaac ttcgatacga cactatcttt caggcgaggg caaatcatct ctgcacgatt
    41881 gagcacattt cagggcagca ccagctttct ccctgagaaa aatggagccg cccgaaagcg
    41941 gctccgaagt aacagggagg cgtcaaacag agtgggcggg ccactcgaca tccagtaacc
    42001 tggataggtt gaagcctagc agagctgtcc taacaagaag taaatccacg gtcgggaata
    42061 acacacctca gcacccgccg aagcccgtga ggccctgccc agagcatttc cgagttagac
    42121 agaaacacgc caagtcgagc tcgcgcccca tctccctctc ccccgtgggg agaggtaaac
    42181 ggagtgctgt tggcgtttcc gttaaagtcg ggaaagctct agacgagagc catcggctgc
    42241 cgattgcgcc cggggcgcgg catgtggtca gcgcttgaag agagctgaag ctgcttgctc
    42301 gtgtgcccgc ttcgccgcga tctcctgctc gaccctctcg atctcttccc gcagagccgt
    42361 gagccgctcc tgcagatcac tcaacgataa cgcatggagg ggctcaccga cggtgatcgt
    42421 gctgcccggc ttcggcctga tctcgtccca atccatgtcg caactcccac tgctcgtcat
    42481 acgcgctggc ggccagcggt gcacctcagc cacaacctaa catgatgacc ttcggcagtg
    42541 gcggcaagat gggtggagcc ggtcgagagg tcgctaccag accagcgccg gaagcgcggc
    42601 aatcggctgc aggctggtgt tgtcgaagag cttggcgacg gccacagcat caagctcgcc
    42661 tggcacatgg atggcgctgg cgatgaagac ggcatcgatc tcggcattcg ctgcgccgcg
    42721 aatgtccgtt ctgaggccat cgccgatcgc caggatccgg cgtcgcggca cgggctcgcc
    42781 ccgcagctct ccgagcctct cgaaggccat ctcatagatc ggcagatgag gtttgccggc
    42841 gtaggtcacc tcgccaccca ggcgctcgta ctccgcggcg agcgacccgg cacagtagac
    42901 gatgcgagag ccacgctcga cggtcagatc gggattggca cagatcatcg gcacgccgcg
    42961 gcgggccagc ggctcgagca tttccgtgta gtcggccggg gtctcggtct cgtcgttgta
    43021 aaggccagag caaatgatga catctgcttc cgccgcctct gtgaaccgca gcttaaggcc
    43081 ctcgaacatc ggcttgtctc tggctggccc gagatggaag agcttcaact gcgcccgctc
    43141 gctgatgagt ttccgcgtga ggtctccaga ggtcacgatg ccgtcataag cctcgcgtgg
    43201 cacgcctaag ccgtcgagct gtgctgcaac ggcgtgggcc ggtcgaggcg cattggtgag
    43261 gaacagcacg gttcccccgc cttcgcgaaa gcgcctgcag gcctcgacgg cgcctgagaa
    43321 cgcacgcacg ccattgtgca tcactcccca gacatcacag atccaggcat catagccgtc
    43381 gacgagcggt gcgatcgagg aaaggagtcg aggggggcgg ggcgctttgg tcatgccgcc
    43441 cccattagcc gcgccacgac tctggatcaa gcggctgcgc tactcgccgc aggacgcagg
    43501 gcctccgccc ggatcgggcg cggcacgccg aagagttgcc cctgacccag cggaacgccg
    43561 gcagctagca cgctttcgag ctgatcctcc ccgccgagac cgacggcgat caaggtcagt
    43621 ccaatctcgg agatgcgctg gcagagctcg gaaatcggca gcaccccttc aaggcttgcg
    43681 agcccgtcga gcatagactg cgcattgatg cgcatgaacg cgaagcccgc ggccttgaga
    43741 tccggcagat cgagatccag actcgtcaca tcctctattg cgaagcgaaa accgctcgtg
    43801 gccagttgcc gaatggtgct ccattgggct gcactcagtc cttgaagctc gctttgcgaa
    43861 atcgagaaga cgagcctctc gcgcagcttc ggtgcctggc ggcacgcgtc ggcaaactct
    43921 tcgagaaaac gcggactggt caaggactcg gccgtgatcg aggagaacag gcaccctcca
    43981 ctgccgcgct cgtccatgtg gcgtgcgacc atggccgccc gcgctgccct ggtggcatcg
    44041 acctgcggca gaaggccggc attgcgagcc atcggaatgt aatcctcggg gccgaagctc
    44101 cccgcgtcgc cattgcgtag acggagcgat atctcgaagt ggcgcgcctt tctgtcggcc
    44161 aacccgacga tcggctccaa gaatacgtcg agcttccccg tcgcgatggc atcggccatc
    44221 gccgccatgt cctcatgctc cggaccgacc ggcgggggtt gcggcttgga agagttggga
    44281 tcgacgcgca gaggcggcag cacgcccttc tcgatcgacg cacgcccgcc ctcgactggc
    44341 cgccgcattt cctctgcggc tgcccggagc gcatccactg aggcagaaat cgccgcttca
    44401 tcggcttcag caggttcacc ggagccagtg cgctcgtccg cagcttcatc actgcttggc
    44461 ttccgcggat gggcgatctc ctcggagaac atccggatca tatcctggat gacctccacg
    44521 tccccctcga cgcctgccgg ctcggcgtcg tcctcgctca cccggccagc cgctacggac
    44581 aaatcatcgg gcttcgagat gctgggccgt gactgtcgag cagctgcacg cgggctctga
    44641 gaaggtccga ccggtgccgc catggtattg gcgcttgccc catgacgagc cggcgcacgc
    44701 ggagccggga gcgtaccggc cgcggggtct tcctgtttgc ttggcgcgac atcggtcggg
    44761 cgaacggacc agaagccaga gatcggatca ccttcgcgcg cgccggaagg ctgaccgccc
    44821 gagctttgat gggaagccaa atgcggggcc cctgccgctg ccgcttttgg ttcgattggg
    44881 tgctgaggcc gcggcgggat gtggttgggt gctgcgggtc tcccctgcat ctgcggcgct
    44941 gggggagctg cgccgcgacg cgcgggagca cctgccgcca tcagctgcgg cggccgcgat
    45001 ccagtggcgc cttcgactgg cgcaggactt tggtatggga acggcaactg ctgagccgaa
    45061 tccactggct gctgctggct tgcaacccga gcaagccgct tcacctcgtt ttcgagacgg
    45121 tcaacttcct gactcaacgc gcgtatggcc tcagaccgac gcaccagaac gtgcgcgcaa
    45181 atgagcccga catagaacgc catcgcgatg agggcggcgt tcaacaaccc tagaccgacc
    45241 tgcaaatgga gcccgaggcc gaccgctagt gaaaccagtg tgatcgccat gaggacgaac
    45301 gcctcgcgcg cggtcccccg acccgcgcga cgacgattcg gtttggtgtc tgctgtcatt
    45361 gcccggcggc cccgaaagga ttgattcctc gactcgtttg aggggaagca ttccgtccgg
    45421 gaagtagcca atgcaatgca tgggcggccg cttctcacca gcttttgcac cagaggcggg
    45481 caccccggcc gaaccagtgg ccaaaaagca tcgcggcggg gagctatccc cgccgcgtgt
    45541 gaactctcac ccgccgttcg cgggctgatt caggcgacct ggcacatcag gcgaccgcct
    45601 tgatcaccgt ggcaatcttg tcacggatct ggccgaaatc gctctcggtc gcgatcagcg
    45661 gcggcgccac gcaaatcgta tctccggaca cccgtacgta caggtcgtgc tcgtgataca
    45721 tgctctcgat cgcgttgtag ccgcgcaagc ccggcttgcc ggggatcggg tcgaggtcga
    45781 tgccgcagat caggccgatg ggccggatgt cgaccacgtt cggcaagccc ctgaggctca
    45841 tcatcgcgtc agcgaacgcc ttctcgttgg cccgcgcctt ctcgaacaat ccctcgtcct
    45901 tgtagacatc gagggtggcg agaccggccg cgacggccag cgggtgaccc gaataagtgt
    45961 acccgtggaa gagctcgatg atgtgctccg gcccctgcat gtgggtctcg tagatcggct
    46021 tgcgcaccat gacgccggac atgggcaccg agccgttggt gacgcccttg gcgaaggtga
    46081 tcatgtcggg aacgattccg tagcgctcgg cggcgaacgg gtagccaagg cgcccgaagc
    46141 cggtgatcac ctcgtcgaag atcagcagaa tcccgtgctt ctccgtgatc tggcgcagcc
    46201 gctccaggta gcccttgggc ggcggcaggc aacccgtcga gccggccacc ggctccacga
    46261 tgacggccgc tatggtcgaa gcgtcatgca gtccgatcag ccgctccagc tcgtccgccg
    46321 tctcggcacc ccattccggc tcaccgaccg agaacgcctg cttggagcgg ttgtaggtcg
    46381 tcggcagatg gtccacaccg gtcagcatgt tgccgaacat cttgcggttg gcgaccatgc
    46441 cgcccaccga gatgccgccg aagccgacgc cgtggtagcc gcgctcacgc ccgatgaagc
    46501 gggtgcggct gccctcaccg cgggagcgat gataagcgag cgccaccttg agggccgtat
    46561 cgacggcctc ggacccggag ttggcaaaca acgtgtactc gaggtcaccc ggcgccagca
    46621 gggacaggcg cgaggacagc tcgaacaccc gcggatggcc gtattggaac ggcggggcat
    46681 agtcgagccg cgccgcggcc tcctggatcg ccttgacgat gggctcgcgg ccatgaccgg
    46741 cgttgacgca gaacaggccg gaggacgcgt cgatgacctt gcgaccgtcc ggcgtcagga
    46801 aatgaagatc cttggcgccg gagaacagcc gcggcttctt cttgaacgag cggttgggcg
    46861 tgaagggcag ccagtaggac tcgagttcgt tgggcttcat atcgagtggc gccattgaat
    46921 tcctccaaag acgattgcgc gggggttgac ggccttcggc gggccattcc ggccacgatc
    46981 tcgcacgccc gcctgcttac aagtagaggc ccgatcagca tagcccatca agcccttttc
    47041 tcgtctttgg tagacccttg attcgtcgac gtccatccgg caaggttttc gatatattca
    47101 acatctgaaa catggagcga tggatgacgt cggtcgatgt cggtgggcgg ctgcgctatt
    47161 tgcgttctcg tcataacctc tcgcagcgag agcttgccaa gcgggcgggt gtgacgaatt
    47221 ccacgatttc gctgatcgaa gcgaacaggg tcaatccgtc cataggcgcg ctgaagcgca
    47281 ttctggacgg tgttccgatc ggcctcgccg agttcttcgc gctcgagccc gaaaagccgc
    47341 gccaggtttt ctacatggcc gaggagctga gcgagatcgg aaagggcggc atctcctatc
    47401 ggcaggtcgg cgagaacctg gtcggccgcg cgttgcagat tttgaaagag cgctacgagc
    47461 ccggtgcaga tacggggcgc gtgccgctgg tgcatgaggg cgaggagggc ggcatcatca
    47521 tcagcggacg cctggaggtg accgtcggct ccgagcgccg tatcctgggt ccgggcgatg
    47581 cctactattt cgaaagccgc cgccctcacc gcttccgcgc tgtcggcccc gttccatgcg
    47641 aagtggtcag cgcctgtacg ccgccatcct tttagcagtg cccgaccgga cagcctatgc
    47701 cgggcaaatc atcgttccag ggtctctgct ctgcgcctag tctctcggtc ggctgtgcac
    47761 ggcctgaatt gcgccgtcct ttggtcccgg cctgacccga atccgggtga catttaggag
    47821 tcgttagtcg aatcggcgtg gagtggcatc gctcgggcgg ggaggcccgg cggtgcgagg
    47881 aggcgatgcc ctatgttgac gagtgtactc gaaagtggcg tatgcgttgg atcctaccct
    47941 gagctggccg gcaaacgcgt tctcatcaca ggattgaatc tcaaggcggg cgtggacata
    48001 gctcgcgcct ttgccgaaca gggttgccgt atcgtactgc acaccaccgc aagcggtacg
    48061 gaagcggatg ccgtcctgga gatgctggcc cagacggcgg cggagttgca ggtctactcc
    48121 gaccctctga cagatgttgc agccaccacg aaattcgcac agacggccgc gcgcaccttc
    48181 agcgggctcg atgctgtcat caacctcttg catgccgacg ttgccgcggt ggtcgcgcaa
    48241 gctgctgacg ccgacatcga gagcaagatc gcaagtcatt tgcagaacgc ccgtctgatt
    48301 acccggattg cggccaatcg gatgcggctg acctggtccg aagggctgat cctcaacgct
    48361 ctggtcgccg caccgcccgt gacgggggcc gaggccatgg tgctcggcat cacgcggtcg
    48421 atgcttgcga gcatgacgag ggccgaggct cagcagtggg ctgaccaggc gatccgcatc
    48481 aacgccatcg caccgtcgac cgacggtccc gacggttgcg agcatctggc ctcggaaccg
    48541 gacatcgcgc tgctcgccat gttcatggcc agcccacggg gccgggaatt ctcaggcttg
    48601 gtgttcgaca ccgccctggc cggcaacgcc cgggaagcag cctgattgaa gtaggtgggg
    48661 gcagccaaga aagctgccct cgtcacgccc ttcaggtcac agctttccgg agagtgcttt
    48721 ctcgtaaagc tcgcggcttg ccgcttcgtc gagcggcaca ggattgccgc cggcggtcgg
    48781 atccttgggc gccatccggc tcatgatgtc gaacttcgag ccgtcgacac cgagccccgc
    48841 taatgtatgc ggcacgcctg tctcctctcg gatttgaagc acccaatcga ggaacgcatt
    48901 gaagtccgcc ttgaggccga gatatgcggc caggcgcgcg atcttgtcct cgatcgccgg
    48961 ccgattgaac atcagcacgt acggcatgaa cacggcattc gtcatgccgt ggtgcgtgtc
    49021 ataaagcgat cccaccgggt gagagagtgc atgaatggcg cccaaggcct tctggaatgc
    49081 cgttgcgccc atggcggcag ccgacatcat gtgggcgcgg gcctcgatgt cctggccgtt
    49141 ggtggcggcg cgcacgaggt tttccttcac gagccgcatg ccctcgaccg caatgccgtc
    49201 cgccatcgga tgatagaacg gcccgcagta ggcctccagg cagtgggcga gcgcatccat
    49261 gcccgtgccg accgtgatcg ccttcggcat gccgaccgtc agctccggat cgcagatcgt
    49321 cactttcggc atcatgagcg ggtggaagat caccttcttg gtatgcgtgg cctcatccgt
    49381 aatcacgccg gcgcggccga cctccgatcc cgtacccgca gttgtcggaa cggcaacgat
    49441 gggcaggatt cctttcgggt cggcgcgggt ccaccaatcg ccgatatcct cgaaatccca
    49501 catcgggcgg gtttgcccgg ccatgaacgc gatcaccttg ccggcgtcga ggcccgagcc
    49561 gccgccgaag gcgacgacgc catcatgatc tcccgctttg agggcagcta cgcccgcctc
    49621 gacattcgcc gcgaccgggt tcggcttcac gtcgctgaaa acctttgcag ggacgccgcc
    49681 agcttccaga acttcgaggg cacgggtcgt gatgggcagc ttcgccagca ccggatcagt
    49741 cacgagcagc ggtcgagaga tgccggccga ttttacggca tcggccagtt cggcgatgcg
    49801 gccagcgccg aaacgtatgg aggtcggata gttccaattg ccagtgaatt tcatggggtt
    49861 cgggcccggt ttgcgttgga taggtgaaat cgccaagtcg agaaatggtg aagtcgtaaa
    49921 atcgtgggaa tattccgatt tcacgacttg gctatttcac gatttcacga gcttcatacc
    49981 tctctcagat gaaaactctt cggccgcgtc agcatctcgt agccgatgcg ggataacccc
    50041 gcgccgcgcc cggtgtcctt gaccccagtc caggccagtg ctggatcgag ataatcgcag
    50101 cggttcatgt agacggtgcc cgtctcgatc tgctggccga tgcgttcggc cgcttcgaga
    50161 tcggttgtcc agaccgaggc tgtcagaccg tagggtgagt cgttcatcag gtgaatggct
    50221 tccgcttcgc cggacacctt catgatcccg accaccggcc cgaagctctc ctccttcatg
    50281 acctgcatgc cgtgatcgac attggtcagc acctgtggtg cgagccaggg cgtgccgtcc
    50341 ttgttgcgcg cgaaagacgc cggatcgata tgggctttgg cacccttccg cagcgcctcg
    50401 gcggtctgct gccggacgac atcggcgaac cgcgtggccg ccatgggacc cagcgtggtg
    50461 gcctcatcga gcggatcgtc gagcacgtac tgcttcgtca gcgcgacgaa gccttccacg
    50521 aacggatcat agatgcgttc gtcgacatag atgcgctcga caccgcagca gctctggccc
    50581 gagttgaaaa acgcgccgtc gacgaggttt tcgatggcgt gatcgagctt cgcatcgctc
    50641 cgcacatagg ccgggtcctt gcctccaagc tcgagcgcga tcgacgtgaa ggagccggcg
    50701 gctgccctct cgatcgcgcg gccgcctgag acgctgccgg tgaaggtcac gtggtcgact
    50761 tcgccatcgc ctaggatgcg cgccgtgtca tcgtggccga gcacgaggtt ttggaacagc
    50821 cccttgggca gcccgacctg atcgaaagcc tgctggaacc gctcgcccgt gaggatggtc
    50881 tgcgaggccg ccttgaggag gacggcgttg ccggcaatga gcgcaggcac gaccgtgttg
    50941 acggcggtca aatagggata gttccagggc gcgatgacca gcacgagccc aagcggatcg
    51001 cggcggatat atcgcttgaa ccccgccttc ggctccggaa cgatcggggc cagcgcctcc
    51061 tcggcaattt cggccatgta ggcgacgcgc tcgctgaagg agcccagctc tccgccgtaa
    51121 cggaccggcc gccccatctg cagcgccagc tcgcgcgtga cctgcgggtt aatggcgcgc
    51181 atggcttcca ggaaccgcag catcagtgcc gcgcgttccc tgaccggcac gcgaacccac
    51241 tcctgccggg ccgtgcgcgc cgcatcgagc gccctctcga tctcagctgc gcttgcggtc
    51301 ggccgccgcg ctacttcccg gccgtcgatg ggcgaaatgc aaacgatttc aggcatgttc
    51361 tttccaatgg gtggggcaaa gactcgcgcc cttgtgactt gccgttgcaa aaaatccggt
    51421 tagacgcagg gacatgttgc taggctaatc aacgcgacga gagtcgatca gcgagaggta
    51481 actgaatgaa gtttcttgct ccatacgaac ctcagcttct gagcgtgctt cggttcatgt
    51541 cgggtctcct actccttcag cacgggacca caaagtatct gaacattccg gttggcccga
    51601 tgaacaacgc atctcccttc acgatgagtg gcgccgcagg cgtcatcgag ctgatcggcg
    51661 gcgtgctcct cgtcatcggc ctgttcacta ggcccgctgc attcattgct tccggcatga
    51721 ccgcagttgc ctacttctat gcccatgccc ctcgcgggtt tttccccatc ctcaacggcg
    51781 gcgagctcgc ggtgctctac tgctttgtgt tcctgtatct cgctgcggcg ggtgggggca
    51841 gatggagcat cgactggatg ttgaaccgca aggatcgctg agaaaagcct ccctttcccc
    51901 attgcacctg cgcaatgggg aaagccgctc tcagtaccgc tcgaagccgc gcatcagctc
    51961 ccagtccgtc acgcggcgat cgtactcgaa ctgctcccag cggccggtgt ggacatagtg
    52021 gtcgaccacg gcgctgccga gcgcctcgcg catgaactcg gacttgtcca aacagtcgac
    52081 cgcctcgcgc agcgtcttcg gcacctcggg gatgccgtcg cggacatagg catcgcctga
    52141 aaacggcgga tcgagctcga gcttctcgtc gatgcccgcg agccccgccg ccagcagtgc
    52201 tgcgaa
//
LOCUS       DASVDU010000268        26416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_200012, whole genome shotgun sequence.
ACCESSION   DASVDU010000268 DASVDU010000000
VERSION     DASVDU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26416
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200012"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF226_17960"
     CDS             <1..328
                     /locus_tag="VF226_17960"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEX7075926.1"
                     /translation="FRHIPAGKQDLFADHVVESMADALNALDGPVLAHCYSGLRSAIL
                     WAAASARSQPVDCVLAAVRKAGFDLDFLRDELEAQADRKRWLGSSPALDCREAARVQD
                     RERVAA"
     gene            complement(372..1025)
                     /locus_tag="VF226_17965"
     CDS             complement(372..1025)
                     /locus_tag="VF226_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX7075927.1"
                     /translation="MVSSSTSKLRSPEADLEALVAQIERCRVCLDAPKGKPLPHEPRP
                     VLRVSATARIAVCGQAPGTRVHASGMPFTDPSGDRLRAWMGVTPEEFYDVSRVAIIPM
                     GFCFPGLDEKGGDLPPRRECAPLWRHELFSRLHHLDLVLLIGQYALRWHLGQLGRRGL
                     TDTVRDWRAVMAYSKRPRYIPLPHPSWRNNAWIKKNPWFEAELLPVLRNEVRRALTH"
     gene            complement(1003..1746)
                     /locus_tag="VF226_17970"
     CDS             complement(1003..1746)
                     /locus_tag="VF226_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7075928.1"
                     /translation="MKLMFIGSGDAFGSGGRLQTCFHVDTGEDAFLIDCGASAMIGLN
                     RFGIDPNTISTIYISHLHGDHFSGLVWFLMHAYYVSKRTEPLTIAGPQGIEERYRAAT
                     EVLFPGSSGIKRKHDVTFVEYGIEQPTRIGPATLTGYEVSHPSGSMSCALRLEVGEKI
                     LSYSGDTQWVDNLIPCAEGADLFIAECYGYDKPVPYHTNWATLSANLPRLGAKRVLIT
                     HMNNDMLARQEEARAAGVLLAEDGLVVDI"
     gene            complement(1747..2373)
                     /locus_tag="VF226_17975"
     CDS             complement(1747..2373)
                     /locus_tag="VF226_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX7075929.1"
                     /translation="MKLIDQPLAPNPRRVRIFLAEKGLTIPAEKVDLAKLEHRSPDFI
                     AKNPFQQVPVLILDDGTAISESVAICRYFEEQHPEPPLFGRDAMERVMVEMWQRRMEL
                     GLLAPVTHCFRHSHPAMAELEKPQVKEWAEVNRPRVLKLLSWLDQELAGRRFIAGDTY
                     TIADITALVAIDFMKPAKIERPADLENVARWYAEVSSRPSAKAGKEAS"
     gene            2754..4331
                     /locus_tag="VF226_17980"
     CDS             2754..4331
                     /locus_tag="VF226_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7075930.1"
                     /translation="MTSSGRVPRSSAADARLRDVRERLARGNTQPSFEYDLLLMFVRN
                     ELSAQATILLLAAIFSLASMFWAPWTQAIMWLFLVIAAKVVLLECCCRFLSTPSAELD
                     TTVWRRRLILAEAINGLAWAGFALVGFNAAEQISQDNLVSANVFVFASLIVVLAIRMT
                     FASTVMPILYVGTIPMTLAVVLRLVLIEDIFYLALAMMAVGVHIYFIFLAKGLRRTTL
                     AMLELRAEKDDLIAELEEAKAISDEARRRAEAANAAKSRFLATMSHELRTPLNAILGF
                     SEVMKSEVMGPLTSPLYKEYATNIHQSGTHLLKLINEILDLSRIEAGRYELNEEPIRI
                     VDIVDDCHRLLKLRAESKGQQIIEQLDEDLPPVWADERALRQVCLNLLSNALKFTPHG
                     GQITLSSGTTEDGGQFFSVRDTGPGIPKEEIPKVLQAFGQGSLAHQTAEGGTGLGLSI
                     VQSLVDLHGGELEIRSELRKGTEAIVRLPRKRVLQAGATMPNSADLKEGANETVHRPS
                     RPPRLKRPAPKLAAGAR"
     gene            4518..5222
                     /locus_tag="VF226_17985"
     CDS             4518..5222
                     /locus_tag="VF226_17985"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX7075931.1"
                     /translation="MLWLLLLLLGAGGLILVLAEDQGTIAGLDPASFAGLIAGGALLL
                     YFSASLLGSYRGRFGQAFRDFAVWVLIAFVLVVAYSFRDEVTYLTKRVAGELLPPGET
                     VVYNGNESGERAVRIRRRPDGHFSIRMQVNGAPVVMMVDTGASTIVLTPADARLAGID
                     VDRLNYSVPVQTANGTAYAAAVRLRDVAVGPIKLDRVDALVAKPGVLNESLLGMNFLR
                     RLRSYEFSGEFLTLRS"
     gene            5272..5838
                     /locus_tag="VF226_17990"
     CDS             5272..5838
                     /locus_tag="VF226_17990"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEX7075932.1"
                     /translation="MRLSSGVRLALREAGKWLLVSLVGIVAIVWFDELKAGISHALAI
                     YLPDRPPPQRITTAAETAPSSPGKVELRAGALGHFSTTAHINGRAVPVLVDTGASMVA
                     LSYEDAERAGIFVHPGDFTAQVRTANGVAKVAPVMLDSVSIGDITIYDVRAAVSEPGR
                     LGTSLLGMTFIGRLSRAEMRGDVLILQE"
     gene            5955..7460
                     /locus_tag="VF226_17995"
     CDS             5955..7460
                     /locus_tag="VF226_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7075933.1"
                     /translation="MTKLPLPRADLKPNTADFERLPLVKPTGFREYDARWLFPDEINL
                     MGLNTIGLGLTTLAAERGVPRRFVVGHDYRWYSSSVKQALITGLLAGGAEVYDIGLAL
                     SPMAYFAQFALDVEGVAMVTASHNDNGWTGIKMGIQRPLTFGPDEMARLKAIVLEGQY
                     QAPGGGKYVFVPDMAERYMKALTDRPKLKRRLKVVAACGNGTAGAFAPQVLEAVGCEV
                     IPLDTELDHSFPRYNPNPEDMKMLHAMAEAVRRHEADVGFGFDGDGDRCGVVDNEGEE
                     IFADKVGVMLARDISAIHPNARFVADVKSTGLFKTDPVLKQNGAQTDYWKTGHSYMKR
                     YSFETKALVGFEKSGHFFFNPPLGRGYDDGLVAALAVCDMLDRAPDKSLADLRRALPR
                     TWQSPTMSPHCDDEKKYEVVDRVVAHYQKLKADGAPVAGQKIAELITVNGVRVMLEDG
                     TWGLVRASSNKPELVVVVESPTSEENMRAIFADIDGVLSKMPEVGEYNQKI"
     gene            complement(7615..8061)
                     /locus_tag="VF226_18000"
     CDS             complement(7615..8061)
                     /locus_tag="VF226_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7075934.1"
                     /translation="MYQHILVPTDGTELSKRAVERSLDLAKALGAKVTVVRVSGKPAH
                     VVVFGVDITELPEETRKRISKEIEEHFAWVRSEAAARGVECEAIRVESEFPWKGIIET
                     AEARGCDLIAMAAHGRAGVTAKLLGSETQRVLTHSRLPVLVYRDGR"
     gene            8333..9100
                     /locus_tag="VF226_18005"
     CDS             8333..9100
                     /locus_tag="VF226_18005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7075935.1"
                     /translation="MDPLPDHEKHLVLQLLDFTRRDKEHNSPEAILNDLHDITFRTCN
                     LNVLGALQFPVRWGDWAKIEKGKNVFLHESAPEGWWREWKAFNRQHPGPSLALAQFAM
                     APFTLTELMQMLEPIGHDRWPFELALKYGIRDALTCPVGGRWAVGFWSRHVLSQRLSP
                     EARAMLFMGATLAAIGLQRLVAPQADRLGNTASLTPRELAVLRALSFGKPFAEIARSL
                     ELGEETVRSHIKKAQAKMQAHNRTHAVAQALRLRLIP"
     gene            9182..9592
                     /locus_tag="VF226_18010"
     CDS             9182..9592
                     /locus_tag="VF226_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7075936.1"
                     /translation="MQHTTWAGRRVLIVEDNTIVVMPLAYFLEDCGAEVIGPAPSLDA
                     AFAAIDDAEQIDGAVLDVELGREMVWPVAFALRERGIPFVFATGSADGSIYPPAFLHA
                     RRFAKPYSEEAVAEGLHALIEDATTADSGLVASE"
     gene            complement(9607..11058)
                     /locus_tag="VF226_18015"
     CDS             complement(9607..11058)
                     /locus_tag="VF226_18015"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7075937.1"
                     /translation="MLDMPFRSARELAAEIKRKSISASELLELFLKRVEVFNPKLNAI
                     VATLPEARERAKAADEALARGEDWGPLHGVPMTVKESFDVVGLPTTWGVPELKENYPT
                     RNALAVDRFLNAGAVLFGKTNVPLYLADWQSFNAVYGTTNNPWDLARAPGGSSGGSAA
                     ALAAGLTGLEAGSDIGASIRNPAHYCGVYGHKPTWGICPPRGQALPGIVAQTDISVIG
                     PMARSADDLELGLSIMAGPDEIDGAGWRLELPASRRSRLGAFKVAVMLDDPCSEVDLA
                     VQDRIAAVADFLAKQGATVSHTARPAVDTREAHALYIALLRAATSARQTDEMVAKNKE
                     IAAHIDPADESYYARMVRANVMLHRDWLAYNNRRHQMRLAWAEFFREWDVLICPAAAS
                     AAFPHDHEGERHERTITVNGKQVPTTDQLFWAGYSGVVYLPSTVAPAGFTPEGLPVGV
                     QIVGPQYGDLTCIALARMLEREYLGFTPPPGFR"
     gene            11263..11751
                     /locus_tag="VF226_18020"
     CDS             11263..11751
                     /locus_tag="VF226_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX7075938.1"
                     /translation="MAKQSRPQKKTVERVMHEYKHGELKSGGRRKVKNPKQAIAIALS
                     EAGASKYQSPAEKRRSLQRTKTKEARGETAQARTEGRGRKTARGGTAGRSGAARRGSA
                     RTSTARSTSARGKTQRSGAARRSTGQERTKAQLYQEAKRRKIPGRSKMSKGELAKAVG
                     QR"
     gene            11872..13344
                     /locus_tag="VF226_18025"
     CDS             11872..13344
                     /locus_tag="VF226_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEX7075939.1"
                     /translation="MRLSDLADGTLDALRNAGEYLADLATPSIRLGVTGLARAGKTVF
                     ITALIRNLTDGGRLPFFDAYAEGRILRAYLQPQPDDDVPRFDYEAHLAAILGDPPRWP
                     ESTRHISELRVTVEYVPSSMLKRAMTTGRLHIDIVDYPGEWLLDLGLIDQSYADWSAL
                     SIALAAKPARSAVSAEWLSLLKSIDPAAPQDEQLALEAARVFTAFLAAARQAEPALST
                     LGPGRFLLPGDLAGSPLLTFVPLQMPEGFTPPRNSMAAMMERRFESYKTHVVKPFFRD
                     HFSRLDRQIVLVDVLAALNAGSAAVDELSSALEASLRAFRPGARSWLASILPRRIDRV
                     LFAASKADHLPSSSHDRLEAVLQLMTEQAAKRVTFAGAMVGVIALSALRATREVEAHQ
                     DGERLACIRGVPMAGEQVGEKVFDGSTEVALFPGDLPADPRQALAPSGRDLTGQVKFV
                     RFRPPRLGGEPGSGVQVWSSAWPHVRLDRALQFLIGDRLQ"
     gene            13341..14360
                     /locus_tag="VF226_18030"
     CDS             13341..14360
                     /locus_tag="VF226_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HEX7075940.1"
                     /translation="MSEPTRPRPPRVFDPDDPSLLQVPEPAADAGGAAEAELAEPELA
                     AGPAPAPRGGIRWGAILGSALLGLAMLAVSLWLTQLATEALSRNDVIGWIAIALVAIA
                     GLAASAIVLRETIGIMRLNRLGRVRREIQDALDARDARREKALLRELLGTFSGRADVR
                     WSVARFREHEADVRDPGDLARLADREVFGPLDARAKRIVLTAAKRISVVTAISPLPIV
                     DVVFVLVENLRMLRGIATLYGGRPGIMAALKLGRMVVTHLVATGGIALTDDLVGQVLG
                     HDLLRRLSQRLGEGAINGALTARIGAAAIQVIRPLPFLETSPVRVRDFLGELFRSTTP
                     VRRRA"
     gene            complement(14289..16304)
                     /gene="mdoH"
                     /locus_tag="VF226_18035"
     CDS             complement(14289..16304)
                     /gene="mdoH"
                     /locus_tag="VF226_18035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX7075941.1"
                     /translation="MSKTSTRRAAQMRTERDAGRSFLPPEQPLDMPAQDFGAPPCPRA
                     QAETQVHWATRAALIAGTLLLTAGFARELYAVLSFVQITPLQIVFLVLSTAAFGWIAL
                     GSLSAAIGLMPLFAGERPDTLEIPATSEPLYHRTALLFPVYHEDCARIAGTVEAMAMD
                     LREMGRAQNFDVFILSDSRSLEAGLREEEVFSALADRLDGTIAVYYRRRLQNTAKKAG
                     NVADWVQRFGGAYDSFVVLDADSVMSGETLVRLAIAMQNRPCAGLIQTVPRLTGGCTI
                     FQRLQQFAAGVYGPAVAAGLAAWHGPQGNYWGHNAIIRTVAFASAAGLPELPGRPPFG
                     GHIQSHDFVEAALLQRAGWEVHMAPSLEGSYEGAPPALPELVARDRRWAQGNLQHLAI
                     VGRRGLTLMARAHLAMGACAYLVSAIWAASLAVGLVLALQGTHVIPSYFLDSKTLFPI
                     WPTIDPGAAFRLFLATMVVVLMPKGIGLILELRRTAARRERFAAPRVIAGVTVETILS
                     MLFAPILMVTQTTAVAQIFAGIDSGWKAQRRDEGDMPFMAAFGYHVWHMAGGLVLAVL
                     TYLVSYSLMAWMAPVLLGLSLSVLLSWWSARTAGPLLSLILATKEDREPPPILELARR
                     RAAAWARYVPGQPLVKLVPLPACASGASADRGGGAEQLAQEIAHAHR"
     gene            complement(16312..17874)
                     /locus_tag="VF226_18040"
     CDS             complement(16312..17874)
                     /locus_tag="VF226_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEX7075942.1"
                     /translation="MANRGVNRRTVLRDAGAWALAAGVISLAMRADWAKAQGPAQPAD
                     SRQPFSPDDVKKIAMELSERPFQRPAMELPEPFDKLTSEQYRDIRFRPEQAIWRGERL
                     DYEIQLFALGYLYDVPVEIWLVEQGIPRQLKADGTLFSIGPRIPDPPQEAPFGFSGFR
                     IHGPINRADMMDEYVVFQGASYFRAVGREEVYGLSARGLAINTAKPPGEEFPFFRSFW
                     IEKPEPGAPHIVVHALLDSPSTTGAYRFTIERGPSTIMDVQATLYPRSEMPHVGLAPL
                     TSMFIHGPAHRRTPNDFRPAVHDSEGLAILNGNGERIWRPLTNPRLLQVSAFVGKDPK
                     GFGLVQRDRAFSSYEDLDAHYERRPTAWVEPAGDWGEGYVELIEIPADEEIHDNIVAY
                     WKPGRPIPAGKPFAFAYRLYWGESVPAAWAGARVRKTRRGSNGRKGTVLFVLDFDGPA
                     LQELADLPVADVSASAGTVSNVVIRRNPMINGVRCSFQLDPGSADLIELRLVLKNLER
                     VISESWLYRWTR"
     gene            complement(18127..18642)
                     /locus_tag="VF226_18045"
     CDS             complement(18127..18642)
                     /locus_tag="VF226_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7075943.1"
                     /translation="MTLRTETYLIRDDGTIPNNDRLPLVVYRSVFGGLARSDNERRFK
                     DLFARHDWGGTWVDGIYPFHHYHARSHEVLGIAAGRAEVQFGGASGPILTLEAGDAVV
                     IPAGVGHCRRSATSDLIVVGAYPRGQEDWDLKRATEAERSQALDEIPKVPLPITDPIT
                     GQSGGLLDLWR"
     gene            18642..19184
                     /locus_tag="VF226_18050"
     CDS             18642..19184
                     /locus_tag="VF226_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075944.1"
                     /translation="MFKARTLRSPGIYVIRRRWFARAGRDCVTGWGMPIEEQTLIAAI
                     MDVRGQVDFLWQFFMTVQIAIFALLFIYDEAVEQLNSFAKFLAIGAVALFDWINSEAL
                     KGAYTLLDALHRQYRADFGQPERFQSVLYDHFVLANFEGRVTMITITHSLAFGVVLMA
                     LLWRRFIQNGEPKNKAATGA"
     gene            19400..20077
                     /locus_tag="VF226_18055"
     CDS             19400..20077
                     /locus_tag="VF226_18055"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEX7075945.1"
                     /translation="MDRARNAALVKRSFQKLIEQLGFSFAICARLSVGQSLEPLSALM
                     CTWPAEFAQAYQAREYWRHDPILQEIIRIRCAVDWPKVFERRRLSPEENKVLEHAAQH
                     GMRAGLAIPITGAGGNVGFVSLCGPQPRCDATCRSVLTLVSIYVFHRLRALAGANGTG
                     QRLSRRELEVLRWIAEGKSDWQIGRILAISAKTVNYHTENVKRKFGVATRMQAVVSAI
                     QRGELTR"
     gene            complement(20318..20734)
                     /locus_tag="VF226_18060"
     CDS             complement(20318..20734)
                     /locus_tag="VF226_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7075946.1"
                     /translation="MRVLVFVKATADSEAGIMPSTELIEAMGKYNEELVKAGIMLGGD
                     GLKPSSQGKRVAFDGANRTVIDGPFAETKELVAGYWIWQVKDMDEAVEWVKRCPNPMP
                     GPSEIEIRPVYELEDFGESEAMERHRKLREQMSKSA"
     gene            20959..22242
                     /gene="egtB"
                     /locus_tag="VF226_18065"
     CDS             20959..22242
                     /gene="egtB"
                     /locus_tag="VF226_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX7075947.1"
                     /translation="MVRQHSVQRESVGGALENRLFATRQLTLELAAPLSAEDMTVQAM
                     DDASPTKWHLAHVTWFFETLILAEHLPGYHLFDEAFAYCFNSYYESLGPRQPRPRRGI
                     LTRPSSERVLAYRMHVDEALSTLFARDIPSDPRVSHLIELGINHEQQHQELLLTDILA
                     LFAANPLRPAYCPRKERAPRPEPDALGWIDYPGGIREVGHAGDGFAWDNETPRHKVFI
                     QPFRLADRLVTNAEWLAFMDDGGYETPSLWLSDGWATVNREGWKAPCYWEQRDGEWLA
                     MSLEGLHPIDPSAPVCHVSYFEADAFARWAGKRLPTEFEWEIAASGLPVRGNTLETRS
                     FRTLPAEPPNGQPRQMFGDVWEWTQSAYLPYPGYRPPAGALGEYNGKFMVSQQVLRGA
                     SCATPPGHTRATYRNFFYPHQRWQFMGLRLASELS"
     gene            22242..23207
                     /gene="egtD"
                     /locus_tag="VF226_18070"
     CDS             22242..23207
                     /gene="egtD"
                     /locus_tag="VF226_18070"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX7075948.1"
                     /translation="MLGQREDRSSEHLAGGNDFRASVLEGLSRPHKSLSSRFLYDAKG
                     SELFEEITRLPEYYPTRTETAILEAHAAEIVDGVPDGSVLVEFGSGSSCKTEILLGKL
                     PRLSAYVAIDVSQSALRAAKLRLASHFPHLEIYPILGDFSDRLTLPASIANRRKIGFF
                     PGSTIGNFEPAEAVRLLNHFRTILSPGGRLVVGVDLKKDPAMLVRAYDDACGVTAAFD
                     LNLLARINREVGGTFDLASFRHKAIYNADEGRIEMHLESKRDQDVRVSDRVFHFRAGE
                     TIHTENAYKYAIAEFRDLARAAQWQPRRVWTDKNNLFSVHELIAA"
     gene            23523..24194
                     /locus_tag="VF226_18075"
     CDS             23523..24194
                     /locus_tag="VF226_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7075949.1"
                     /translation="MEFLADYLTTGALTAFGQVVFIDLVLAGDNAIVIGLAAAGLPAG
                     QRNKAILVGIMAATILRIGFAAITVQLLAIVGLLLAGGLLLLWVCWKMWRELRVSAEV
                     AHSGQEALANADLDKSGGIAEGAPTKTFAQAALQILIADVTMSLDNVLAVAGASREYP
                     LVLVFGLVLSIALMGVAAAYIAKLLHRHRWIAFVGLAVILYVALDMIWRGANDVWPHI
                     EAAIM"
     gene            complement(24243..25271)
                     /locus_tag="VF226_18080"
     CDS             complement(24243..25271)
                     /locus_tag="VF226_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7075950.1"
                     /translation="MPGQNRPLHVSLVAIPDAVVSTLTGIYDVLGAFRMLGRVDPSLP
                     TEPPFKVEIVSTRAGSVSLASGVPVEAPRAAAEIEATDIVIVPSILLGPDGWQVGRYP
                     ELVDWVSRMHARGAVLCSACSGVFLLAETGLFDGRNTTIHWGYADAFARVFPNIPLQP
                     ERVLVVSGEREELITSGASMTWHDLVLYLIVRYVGATAAQAIARSFALQWHQDGLAPF
                     IVFEGRKDHGDAAIAAAQEWLATHFSVASPVEEMVRRSGLSERTFKRRFRQATGLAPI
                     EYVQRLRIEDAKRRLERTDASADEISWKVGYEDAAFFRRLFKRTTGLTPGAYRRRFQL
                     PEFARREG"
     gene            25396..>26416
                     /locus_tag="VF226_18085"
     CDS             25396..>26416
                     /locus_tag="VF226_18085"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7075951.1"
                     /translation="MTVNQQKLNMLVERLFGDLSAGYGGVMVSIGDKLGLYKAMAGAG
                     PLSSQEVARRAGCAERYVREWLNSQVAGGYIDYHPTSATYELTPEQAAVLADDSSPVF
                     LPHAWQVVASMWADEPKSRAAIKTGRGMSWGEHDERLFCGVAAFYRNAYSASLVSEWL
                     PALTGVTEKLEKGAMVADVGCGHGHSTVLMAKAFPKSRFWGFDAHEGSVAAAQEIARE
                     AGVGDRVTFEVAKADTYPARGYDLICFFDCLHDMGRPVDAVRHAMKAVARDGTLMLIE
                     PFARDRVEENINPIGRLYYAASTTMCCAHAISENGTHVLGAQAGQRRLEDVLKEAGLK
                     TIRKAV"
BASE COUNT         4658 a         8578 c         8489 g         4691 t
ORIGIN      
        1 gttccggcat atccccgccg gcaagcagga tctgttcgcg gaccacgtgg tcgagtcgat
       61 ggcagatgcg ctgaacgccc tggacggccc ggtcctcgcc cactgctatt cggggctgcg
      121 ctcggccatt ctctgggcgg cagccagcgc gcgcagccag cccgtggact gcgtgctcgc
      181 agcggtcaga aaggccgggt tcgatctcga cttcctgcgg gatgagctcg aagcgcaagc
      241 cgatcgcaag cgctggctgg ggtcgagccc tgctctcgac tgccgggagg ccgctcgagt
      301 tcaggatcgc gaacgggttg cagcctgacg gtccgcaggc ccgcgcatcc gtggaacctg
      361 ggcttccaaa atcaatgggt gagcgcgcgc cggacctcgt tccgtaagac gggcagcagc
      421 tcggcctcga accacgggtt cttcttgatc caggcgttgt tccgccacga cgggtgcggc
      481 agcgggatgt agcgcggccg cttcgagtag gccatcaccg cccgccagtc acgcaccgtg
      541 tccgtcaggc cacggcggcc gagctgcccc agatgccagc gcagcgcgta ctggccgatg
      601 aggagaacaa gatcgaggtg atggagccta gagaacagct cgtgccgcca gagcggtgcg
      661 cactcccggc gcggcggcag gtcgccgcct ttttcatcaa gtccgggaaa gcaaaaaccc
      721 atcgggatga tggccactcg gctgacgtcg tagaactcct cgggcgtcac gcccatccat
      781 gcccgcaggc ggtcgccgga agggtccgtg aacggcatgc ccgaggcatg cacccgcgtc
      841 cccggcgcct gtccgcagac ggcgatccgc gccgttgcgc tgacgcgtaa caccggccgc
      901 ggctcgtgcg ggagcggctt gcccttgggc gcgtcgagac agacgcggca gcgctcgatt
      961 tgcgcgacaa gggcctccag gtcggcttcc ggcgaccgca gcttagatgt cgacgacgag
     1021 accatcctct gccagcagta cgcctgccgc ccgggcttct tcctgcctgg ccagcatgtc
     1081 gttgttcatg tgcgtgatga gcacgcgctt cgctccaagc cgcgggaggt tggccgacaa
     1141 cgtggcccag ttcgtgtggt agggcacggg cttgtcgtag ccgtagcatt ccgcgatgaa
     1201 caggtcggcg ccctcggcac acgggatgag attatccacc cactgcgtgt cgcccgagta
     1261 ggacaggatc ttctcaccta cttccaggcg aagcgcgcac gacatggaac cggacgggtg
     1321 agacacctcg taacccgtca gagtggccgg gccgatccgc gtgggctgct cgatcccgta
     1381 ctcgacgaac gtcacgtcgt gcttgcgctt gatgccgctc gagcctggga acagaacctc
     1441 ggtggctgcc cgataccgct cctcgattcc ctgcggcccg gcgatggtca gcggctccgt
     1501 tcgcttgctc acgtagtatg cgtgcatgag gaaccatacg agcccgctga aatggtcccc
     1561 gtgcaggtgc gagatgtaga tggtgctgat cgtattggga tcgatgccga agcggttcag
     1621 cccgatcatg gccgaagcgc cgcagtcgat cagaaaggca tcctcgccgg tatcgacgtg
     1681 gaagcaagtc tgcagccgcc cgcccgaccc gaacgcatcg ccactgccga tgaacatgag
     1741 tttcattcag gaagcctcct tccccgcctt ggcgctcggc cgcgatgaaa cctccgcgta
     1801 ccagcgggcg acattctcca gatctgcggg acgttcgatc tttgccggct tcatgaagtc
     1861 gatggccacc agcgcggtga tgtcggcaat ggtgtacgtg tcgccggcga tgaagcgtct
     1921 gccggcgagc tcctggtcga gccaggacag cagcttgagc acgcgcggcc ggttgacttc
     1981 ggcccactcc ttcacctggg gcttctccag ctcggccatc gcgggatgcg agtgacggaa
     2041 gcagtgcgtg accggcgcaa gcaagccaag ctccatccgg cgctgccaca tctcgaccat
     2101 gacgcgttcc atggcatccc ggccgaacag cggcggctcg gggtgttgtt cctcgaaata
     2161 gcggcagata gcgacgcttt cggaaatggc ggtgccgtca tcgaggatga gcaccggcac
     2221 ctgctgaaac ggattcttcg caatgaagtc cggtgaccgg tgctcgagct tggccagatc
     2281 caccttctct gccgggatcg tgaggccctt ctcggccagg aagatgcgga cgcgccgcgg
     2341 attgggcgcc agcggctgat cgatgagttt caaagggtcc tcctcggacg acggtaatgg
     2401 aacgggacgc tgcacgcgcc agcgcgaaat ctttcacggc gggctcgccg cgccaatccc
     2461 cctcggcgcg gcattgcagc ccatggccac ggacacctgg cctgccgcgc cacaccgggg
     2521 caagggaatc ggctcctttc tccaaccctc tctgcggcaa tccttcctca atctcccgat
     2581 gaaataagtg ccaaccactc tcaagacgaa ctcgaagtaa acggggaatt agtaacttcc
     2641 gtggacagtt gtaacccggg gctgcggatg ggttgcaacg tgccggaagg tgaccaccct
     2701 ccagcccttc ggcgagtcgc ccacgattgg cggactaaat ggccagtgat gcgatgacaa
     2761 gttcgggccg tgtgccccgc agcagcgccg cggatgcacg gctccgcgac gtgcgcgagc
     2821 ggctggcacg gggcaataca caaccgagct tcgagtacga cctgctgctg atgttcgtcc
     2881 gcaacgaact gtcggctcag gcgaccattc tgcttcttgc ggccatcttc tccctggcgt
     2941 cgatgttctg ggcgccctgg acacaggcca tcatgtggct gttcctggtc atcgccgcca
     3001 aggtggtgct tctggaatgc tgctgccgct ttctgtcgac gccgagcgcg gaactcgaca
     3061 cgaccgtatg gcgccgccgt ttgatcctgg cggaggcgat caacggcctg gcctgggccg
     3121 gcttcgcgct cgtcggcttc aatgcggcgg agcagatctc gcaggacaac ctcgtttcag
     3181 cgaatgtctt cgtgttcgcg tcactgatcg tcgtgctggc gatccggatg acgttcgcct
     3241 cgacggtgat gccgattctt tatgtcggca ccatcccgat gacgctcgcc gtcgtcctgc
     3301 ggctcgtgct gatcgaggac atcttctatc tggcgctggc gatgatggct gtcggcgtcc
     3361 acatctattt catcttcctg gcgaaaggcc tccgccgcac gacgcttgcc atgctcgagc
     3421 tgcgcgcgga aaaggacgac ctcatcgccg agctggagga agcgaaggcc atctcggacg
     3481 aggcccgccg gcgcgccgag gccgccaacg ccgccaagtc ccggttcctg gccacgatga
     3541 gccacgagtt gcgcacgcca ctgaacgcaa ttctcggctt ctcggaggtc atgaaatccg
     3601 aggtgatggg gccgctgacc agccctctct acaaggaata cgcgaccaac atccatcaga
     3661 gcggcacgca cctgctcaag ctgatcaacg agattctcga tctgtcgcgc atcgaggccg
     3721 gccgctacga gctgaacgag gagccgatcc ggatcgtcga catcgtcgac gactgccacc
     3781 gcctgctgaa gctcagggcg gaaagcaaag gccagcagat catcgagcaa ctcgacgagg
     3841 acctgccgcc ggtgtgggcc gacgagcgtg ctttgcgtca ggtctgcctg aacctgctgt
     3901 ccaatgcgtt gaagttcaca ccccacggcg gccagatcac gctgtcgagc ggcacgacgg
     3961 aggacggcgg gcaatttttc agcgtccgcg atacagggcc ggggattccc aaggaggaga
     4021 tcccgaaagt gctgcaggcg tttggccagg gatcgctcgc gcaccagacc gcagagggcg
     4081 gcaccggcct gggtctatct attgtgcaga gcctggtcga cctccacggc ggtgagcttg
     4141 aaatccgttc cgagcttcgc aaaggcaccg aagcgatcgt gcgtctgccg cgcaaacgcg
     4201 tgctgcaggc gggggccacc atgcccaata gcgcggatct caaagaaggc gccaatgaga
     4261 ctgtgcaccg gccgtccaga ccgccgcgtc tcaagcggcc cgcccccaag ctcgcggccg
     4321 gcgcacgcta gctcaacaca tttgcctctc cagagtaccg gcacgagaag gctgcgcatt
     4381 gcgctcgctt gcccggcccc ctagattcct gggccagatg aacactcgtg aaagcgacgg
     4441 cacgtgggcc tcttgtggca cgagctgccc ggcatcggcc gctcccgagc gcgcgcggcc
     4501 aagggcgggg tgatcgaatg ctgtggcttc ttctgctgct gctcggggcc ggtgggctca
     4561 ttctggtcct cgccgaggat cagggcacca tcgcgggcct cgacccggcg agcttcgccg
     4621 gactgattgc cggcggggcg ctgcttctct acttctccgc ctcgctcctg ggctcctacc
     4681 ggggccgctt cggacaggca ttcagggatt tcgccgtctg ggttttgatc gcgttcgtgc
     4741 tcgtcgtcgc gtacagcttc cgtgacgagg tcacgtacct gaccaagcgc gtcgccggcg
     4801 agctgctgcc gcccggcgag acggtcgtct acaatggcaa tgagagcggg gaacgcgccg
     4861 tccgcatccg ccgccggccg gatgggcatt tctccattcg catgcaggtc aacggcgcac
     4921 ccgtggtgat gatggtcgat acgggtgcct cgaccatcgt gttgacgccg gcggatgcac
     4981 ggctcgccgg gatcgacgtc gaccggttga attactcggt gcccgtgcag accgcgaacg
     5041 gcaccgccta cgccgcggcc gtgcgcttgc gggacgtcgc cgtcggaccg atcaagctcg
     5101 atcgcgtcga cgcgctggtg gccaagcccg gtgtgctcaa cgagagtctt ctcggtatga
     5161 acttcttaag gcggctgcgc tcttatgagt tctctggaga gtttctgacg ctgcgcagct
     5221 gaccggttcc ggcatccgca gcggcgtctc gaacggctgc ggatcttggt catgcgtctg
     5281 tcgtccggcg tgaggctcgc attgagagag gcgggaaaat ggctgctcgt ctcgctcgtc
     5341 ggcatcgttg cgatcgtctg gttcgacgag ctcaaagccg gcatctcgca cgcgctcgcg
     5401 atctacttgc ctgaccgccc cccgccacag cgtatcacaa cagctgcaga gaccgcgcca
     5461 agctctcccg gcaaggtgga gctgcgtgcc ggcgcactcg gccatttcag tacgacggcc
     5521 cacatcaatg gccgcgccgt ccccgtgctc gtcgacaccg gcgcgtccat ggtggcgctg
     5581 agctatgagg atgcggagcg ggccggcatt tttgtgcacc ccggggactt cacggcacag
     5641 gttcgcaccg ccaacggggt cgcgaaagtc gcacccgtca tgctcgacag cgtcagcatc
     5701 ggcgacatta cgatctacga cgtgcgggct gccgtgagcg agcccggccg gctgggaacc
     5761 agccttctcg gcatgacgtt catcggtcga ctcagccgcg ccgagatgcg gggcgacgtc
     5821 ctgatcctgc aggaatagga cccggccttc ggctcgccta taatctttat tcttgatatg
     5881 ccgacctcgc ccaatccgtg cgggccgccc ggcggcctgc attcccgcgc agagatgact
     5941 ggagaagagt tacgatgacg aagcttccct tgccgcgcgc cgacctcaag cccaatacgg
     6001 ccgatttcga gcgcctgccg ctcgtgaaac ccaccggatt ccgcgagtat gacgcacgtt
     6061 ggctgttccc cgacgagatc aacctcatgg gtctcaacac catcgggctg gggctgacga
     6121 cgctggccgc cgagcggggc gtgccgaggc gtttcgtcgt cggtcacgac taccgctggt
     6181 attcctcctc ggtgaagcag gccctgatca cggggctgct ggccggcggc gcggaggtct
     6241 acgacatcgg cctcgcgctc tcaccgatgg cctacttcgc ccagtttgcc ctcgacgtcg
     6301 aaggcgtggc catggtcacg gccagccaca acgacaacgg ctggaccggc atcaagatgg
     6361 gcattcagcg cccactgacg ttcggacccg acgagatggc acggctgaaa gccatcgtgc
     6421 tggagggtca ataccaggcg ccgggcggcg gcaagtacgt gttcgtccct gacatggccg
     6481 agcgctacat gaaggcgctg acggaccgtc cgaagctgaa gcggcggctc aaggtcgtcg
     6541 ctgcctgcgg caacggcacg gcaggcgcgt tcgcgccgca ggtgctcgag gcggtgggct
     6601 gcgaggtgat ccctctcgac accgagctcg accactcctt tccgcggtat aatccgaacc
     6661 cggaagatat gaagatgctg cacgcgatgg ccgaagcggt gcggcggcac gaggcggatg
     6721 tcggtttcgg cttcgacggc gatggcgacc gctgcggcgt cgtcgacaac gagggcgagg
     6781 agatttttgc cgacaaggtg ggggtcatgc tcgcgcgcga catctcggcc atccatccca
     6841 atgcgcggtt cgtggccgac gtgaagtcga caggtctgtt caagaccgat cccgtcctca
     6901 agcagaacgg cgcccagacg gactactgga agaccggcca ctcctacatg aagcgctaca
     6961 gcttcgagac gaaggcgctg gtcggcttcg agaagtcggg ccacttcttc ttcaacccgc
     7021 cgctcgggcg gggatatgac gacgggctcg tcgcggcgct cgccgtctgc gacatgctgg
     7081 atcgcgcacc ggacaaatca ctcgccgatc ttcggagggc actgccgagg acgtggcagt
     7141 cgcccaccat gtcaccccac tgcgacgacg agaagaagta cgaggtggtc gaccgggtgg
     7201 tcgcgcatta tcagaagctg aaggccgacg gcgcgccggt agcgggtcag aagattgccg
     7261 aattgatcac cgtcaacggc gtccgcgtca tgctcgagga cgggacttgg ggcctcgttc
     7321 gcgcgtcgtc gaacaagccg gagctcgtcg tcgtcgtcga aagcccgacg tcggaagaaa
     7381 acatgcgcgc catcttcgcc gacatcgatg gggtgctgtc gaagatgcct gaggtaggcg
     7441 agtacaatca gaagatctga tcaggagtct gcgatgccgg cggactagcc gctcctacca
     7501 tcctgagggt ccgaccgttt actgatgttg ccgctcctca ccggcccttc agatcgcctt
     7561 cgatcaggtt cgggacaggc tctctcacgc ggagaggcag aggttccaac gatctcaccg
     7621 cccgtcacga tagaccagca cggggaggcg gctgtgggtt aggaccctct gcgtctcgct
     7681 gccgagcagc ttcgcggtca cgccggcgcg gccgtgagct gccatcgcga tgaggtcaca
     7741 gccgcgcgcc tcggcagtct cgatgattcc cttccaggga aactcgcttt cgacgcggat
     7801 tgcctcgcac tcaacgcctc ttgccgccgc ctcgctgcgc acccatgcga aatgctcttc
     7861 gatctccttg gaaatccgtt ttctcgtttc ctctggaagc tccgtgatgt cgacgccgaa
     7921 cacgacaacg tgggctggct tgcccgagac gcgaacaacc gtgactttcg ccccgagcgc
     7981 cttcgcgaga tccaggctgc gctcgacggc gcgcttagac agctcggtgc cgtcggtagg
     8041 aacgagaatg tgttggtaca tgtcttctcc tgccatctgg cctctctggc gagactattt
     8101 cgaccccaac tgcgtttcca ggcgaaactc ggctcgcggc cgcagagcct ggatgatcgc
     8161 cgggatcaat gggcgctttg atcggcatca agcggcgggt cgcagcatca tctggtccgg
     8221 gcatcagcca acccgggcgt taaccgggca cagctcgtcc tgttgttgct gagtcgatct
     8281 tgcatcagca tccacgtgcc acgcctgctt agtcaaagac ggggccgacc caatggatcc
     8341 ccttccagac cacgagaaac acctcgtcct gcagctcctt gacttcaccc ggcgagacaa
     8401 ggagcacaac agccccgaag caatcctcaa cgacctccac gatatcacat tccggacctg
     8461 caatctgaat gtcctcggcg ccctccaatt tcccgtgcgc tggggagatt gggcgaagat
     8521 cgagaagggc aaaaacgtct ttctccacga aagtgctccc gagggctggt ggcgcgagtg
     8581 gaaggcgttc aacagacagc atcctggtcc gtcgctggcc ctcgctcaat tcgccatggc
     8641 tccctttaca ttgaccgagt tgatgcagat gctggagccg atcggccacg acagatggcc
     8701 tttcgagctc gcgctgaaat acggcatccg cgacgcgctc acctgtccgg tgggcggccg
     8761 ttgggccgtt ggcttttggt cgcgccacgt tctctcgcag cgcctcagcc cggaagcacg
     8821 cgcaatgctc ttcatgggag cgacgctcgc ggccattggc ctgcagcggc tcgttgcgcc
     8881 gcaggccgac cggttgggaa atacggcgtc ccttacgccg cgtgagctcg ctgtcctgcg
     8941 cgcattgtcg ttcggcaagc ctttcgccga gatcgccagg tcactggagc tcggtgagga
     9001 gacggtccgc tcgcatatca agaaggcgca ggccaagatg caggcgcaca accgcaccca
     9061 tgctgtcgcg caggcgctga ggctccgcct gatcccctag cgatgcgggt ctccccccga
     9121 tcggggatgg tatcggccat caaaacgcgc atcttgcccg atgaactggt gggagaagct
     9181 gatgcagcat actacctggg caggtcggcg ggtgctcatt gtcgaggaca atacgatcgt
     9241 tgtgatgccg ctcgcctatt ttctcgagga ctgcggcgcc gaagtcattg gccccgctcc
     9301 gtcccttgat gcggctttcg cggcgatcga cgacgccgaa cagatcgatg gcgcagtgct
     9361 cgatgtggag ctcggcaggg aaatggtctg gccggtcgcg tttgcactcc gtgagcgcgg
     9421 gatcccgttc gtattcgcca cgggatcggc cgacggttcc atttatccgc cggctttctt
     9481 gcatgcccga cggtttgcca agccttactc cgaggaagcg gtggcagaag ggctgcacgc
     9541 gctgatcgag gatgcgacca ccgcggacag cgggctcgtc gcatctgagt gagctgctgg
     9601 cacccgtcac cggaatcccg gcggcggagt gaagcccaga tactccctct cgagcatccg
     9661 ggccagcgcg atgcacgtca ggtcgccgta ctgcggcccg acgatctgca cgccgaccgg
     9721 caggccctcc ggcgtaaatc ctgccggcgc gaccgtcgac ggcagataga ccacgcccga
     9781 gtagcccgcc cagaagagct ggtccgtggt cggtacctgc ttgccgttga cggtgatcgt
     9841 gcgctcgtgg cgctcacctt cgtggtcatg ggggaacgcc gcggaggcag cggcggggca
     9901 gatgagcacg tcccactcgc ggaagaactc ggcccaggcg agccgcatct gatggcgcct
     9961 gttgttgtag gcgagccaat cacggtgcag catgacgttg gcccgcacca tgcgcgcata
    10021 gtagctctcg tctgcaggat cgatgtgggc ggcgatctcc ttgttcttcg ccaccatctc
    10081 gtcggtctgg cgcgctgagg tggcagcacg cagcagcgcg atgtagaggg cgtgcgcttc
    10141 ccgtgtgtcg acggcaggcc gggcggtatg gctgacggtc gccccctgct tagccagaaa
    10201 atccgcgacg gcagcgatgc ggtcctgcac ggcgagatcc acctcggagc aaggatcgtc
    10261 gagcatcacg gcaactttga acgcgccgag gcggctccgg cgcgaggccg gcagctcgag
    10321 ccgccagccc gcgccatcga tctcgtccgg ccccgccatg atcgagaggc caagctccag
    10381 atcatctgcg ctgcgcgcca tcggcccgat gacggaaatg tcggtctgcg cgacgatgcc
    10441 gggcagggcc tggccgcgcg gcgggcagat gccccaggtc ggcttgtggc cgtagacacc
    10501 gcagtaatga gccggattgc ggatggacgc gccaatgtcg ctgccggctt ccagtccggt
    10561 gaggccggca gcaagggctg ctgccgaccc gccggatgag ccgccgggcg cgcgcgcgag
    10621 atcccaggga ttgttcgtgg tgccgtagac ggcgttgaac gactgccagt ctgccagata
    10681 gagcggcacg ttcgtcttgc cgaacaggac cgcacctgcg ttgaggaagc gatcgacggc
    10741 gagcgcattg cgcgtgggat aattctcttt cagctccggc acgccccagg tcgtcggtag
    10801 ccccacgaca tcgaagcttt ctttgaccgt catcggcacg ccatgcagcg ggccccaatc
    10861 ctcgccgcgc gcgagtgcct cgtcggcggc tttcgcgcgt tcgcgcgcct cgggcagcgt
    10921 cgcgacgatc gcgttgagtt tcggattaaa cacctcgacg cgcttcaaaa acagctcgag
    10981 aagctcgctc gcgctgatcg atttgcgctt gatctctgcg gcaagctcac gcgccgagcg
    11041 gaacggcatg tcgagcatct catatctcca tcgcgactga gacttccatc atcggcgact
    11101 tccatcatcg gccgaaccgg cgatcgcggg caagccatct ccttcagcag acgtcggacg
    11161 atctcgatac gtatcccgcg ggtcgagcga tagggaacgc tattgtgcgg cgcacgttgc
    11221 agccacacgc attggcatcc gcaaacacga gcgagtgata cgatggcgaa acagagcagg
    11281 ccgcagaaga aaacagtcga gcgcgtcatg cacgagtaca agcatggcga actcaagagc
    11341 ggtggccgcc gcaaggtgaa gaatccgaag caggcgattg cgatcgcgct gagcgaggcc
    11401 ggggcatcga aataccagag tcccgccgag aagcgccgca gcctgcagcg gacaaagacg
    11461 aaggaagcac gcggtgaaac ggcccaggcc cgcacggaag gccgtggtcg caagaccgcc
    11521 cgcggtggca ccgccggtcg aagtggggcg gcgcgccgtg gcagtgcacg caccagcacc
    11581 gcacgcagca cgtctgcacg cggcaagaca cagcgtagcg gagctgcccg caggagcacc
    11641 ggccaggaac ggacgaaggc gcagctctac caggaagcga agcgccgcaa aatcccgggc
    11701 cggtcgaaga tgtcgaaggg cgagctggcc aaagccgtcg ggcagcgttg atacagattt
    11761 ccgcccgcga tttcgcgcgt cgccactgac gcgaggcatc ttgcagcctt gtgctgatgc
    11821 cgcgatcggg ccagtttgcg ccacgcggca accacaggtg agcacgacgc cttgcgcctg
    11881 agcgatcttg cagacggtac actcgacgct ctgagaaatg ccggcgagta cctcgccgat
    11941 ctcgcaacgc cctccattcg gctcggcgtg acgggtctcg cccgcgcggg caagaccgtt
    12001 ttcatcaccg cgctcatccg caatcttacc gacggcggcc ggctgccgtt cttcgatgcc
    12061 tacgccgagg gccgcattct gcgcgcctac ctgcagccgc agcccgacga cgatgtccct
    12121 cgcttcgatt acgaagccca tctcgccgcg attctcggcg acccgccgcg ctggcccgaa
    12181 agcacgcggc atatcagcga gctgcgggtc actgtcgagt acgtcccgtc gagcatgctg
    12241 aagcgggcga tgacgacggg gcggctgcac atcgatatcg tcgactatcc cggtgaatgg
    12301 ctgctcgacc tcggcctgat cgaccagagc tatgcggact ggtcggccct gtcgatcgcg
    12361 ctcgccgcaa agcccgcgcg cagcgccgtc agcgcagagt ggctaagcct tctcaagagc
    12421 atcgatccgg cagcaccgca ggacgagcag ctggccctcg aagcggcgcg cgtcttcacc
    12481 gccttcctcg ccgctgcccg ccaggctgag cccgcgctct cgacgctggg acccggtcgc
    12541 ttccttctgc caggcgacct tgccggctcg ccgctgctga cgttcgtgcc gctccagatg
    12601 ccggagggat tcacgccgcc gcgcaattcc atggccgcga tgatggagcg gcggttcgaa
    12661 agctacaaga cgcacgtcgt gaagccgttt ttccgcgacc acttcagcag gctcgaccgg
    12721 cagatcgtgc tcgtggacgt tctggcggcg ctcaacgctg gcagtgctgc ggtcgacgag
    12781 ctcagcagcg cattggaggc atcgctgcgc gccttccgcc ccggtgcacg aagctggctc
    12841 gcgagcatcc tgcctcggcg gatcgatcgc gtgctgttcg cggcgagcaa ggccgatcat
    12901 ctgccgagct ccagtcacga ccggctcgag gccgtcctgc agctcatgac ggagcaggcc
    12961 gccaagcggg tcacgtttgc gggggccatg gtcggcgtca ttgcgctttc cgcgctgcgg
    13021 gcgacgcgcg aggtcgaagc ccatcaggac ggcgagcgac tggcctgcat ccgcggcgtg
    13081 ccgatggccg gggagcaagt cggcgagaaa gtgttcgacg gctcgaccga ggtcgcactg
    13141 tttcccgggg atctgccggc cgatccgcgt caggcgcttg cgccgtcggg gcgggatctc
    13201 accgggcagg tgaagttcgt gcgtttccgc ccaccgcgcc tcggcggcga gccgggcagc
    13261 ggcgttcagg tgtggtcgag cgcctggccg cacgtgcggc tcgaccgggc gctccaattc
    13321 ctcatcgggg atcggctgca atgagcgagc ccaccagacc gcgcccgccg agagtgttcg
    13381 atccggacga tccgtcgctt ctgcaggtgc cggagcccgc cgccgatgcg gggggcgccg
    13441 ccgaagcgga gctggccgag ccggagctcg cagccggccc ggcgccggcg ccgcgcggtg
    13501 ggatccgctg gggcgccatc ctgggctccg cgcttctcgg tctggcgatg ctcgccgtca
    13561 gcctctggct gacacagctc gcgacggagg ctctgtcgcg caacgacgtc atcggctgga
    13621 ttgccattgc gctggtcgcc atcgccgggc tcgcggcaag cgccatcgtg cttcgggaga
    13681 caatcgggat catgcggctc aaccgcctgg gtcgcgtgcg ccgggagatc caggacgcgt
    13741 tggacgcgcg ggatgcaagg cgcgaaaagg ctctgctgcg cgagctcctc ggtacgtttt
    13801 cagggcgggc ggacgttcgc tggagcgttg cccgcttccg cgagcacgag gctgatgtgc
    13861 gcgatcctgg cgatctcgcc cggctcgccg accgcgaggt cttcgggcca ctcgacgcac
    13921 gcgccaagcg catcgtgctc acggcggcga agcggatcag cgtcgtcacg gcgatcagcc
    13981 ccttgccgat cgtcgatgtc gtgttcgtcc tcgtcgagaa cctgcgcatg ttgcggggca
    14041 ttgccaccct ctacggcggc cggcccggca tcatggcagc gttgaaactc gggcggatgg
    14101 tggtcacgca cctcgtcgcg accggcggga tcgcgctgac cgacgacctc gtgggtcagg
    14161 tcctggggca cgacctgctg cgccgtctct cgcaacggct cggagagggc gccatcaatg
    14221 gagcgttaac ggcgcgcatc ggcgcggcgg cgatccaggt gatccgtccc ctgcccttcc
    14281 tcgaaacgtc acctgtgcgc gtgcgcgatt tcctgggcga gctgttccgc tccaccaccc
    14341 cggtccgccg acgcgcctga agcacaggcc gggagcggga cgagcttcac gagcggttgg
    14401 ccgggcacgt agcgcgccca ggcggccgca cgccgacgcg ccagctccag aatgggggga
    14461 ggctcgcgat cctctttcgt ggcgagaatg agcgacagca gcggccctgc cgtgcgcgcc
    14521 gaccaccagc tcagcaaaac cgatagggac agcccgagca gaacaggcgc catccaggcc
    14581 atcaggctgt aggagacgag ataggtcagc acggcgagga ctaggccacc ggccatgtgc
    14641 cagacgtgat agccgaaagc ggccatgaac ggcatgtcgc cctcgtcacg gcgctgcgcc
    14701 ttccagcccg aatcgatgcc ggcgaaaatc tgggcaaccg ccgtcgtctg cgtcaccata
    14761 aggatgggcg cgaagagcat cgaaaggatc gtctcgacag tgaccccggc gatgacgcgc
    14821 ggcgccgcaa accgctcccg ccgcgccgca gtccggcgca attccaggat cagcccgatg
    14881 cccttcggca tcagcaccac gaccatggtg gcgaggaaca ggcggaacgc ggcgccggga
    14941 tcgatggtcg gccagatcgg aaacagcgtc ttgctgtcga ggaagtagga cgggatgacg
    15001 tgcgtcccct gcaacgcgag cacgaggccg acggcgagcg aggctgccca gatcgcagaa
    15061 accaggtagg cacacgcgcc catggccagg tgcgcacgcg ccatcagcgt cagcccgcgc
    15121 cggccgacga ttgccaggtg ctgcaggttt ccttgcgccc agcgccggtc tctggcgacc
    15181 agctccggca aggcgggcgg cgcgccttcg taggaccctt cgagcgaagg cgccatgtgc
    15241 acctcccagc ccgcacgctg cagaagcgcg gcctcgacga agtcgtggct ctgaatgtgc
    15301 ccgccgaacg gcggccggcc aggaagctcg ggcagaccgg cggcagaggc gaaagccacc
    15361 gtgcggatga tggcgttgtg gccccagtag ttgccctgcg gcccatgcca cgcggccagg
    15421 cctgccgcta cagcggggcc atagacgccg gcagcgaact gctgcaaccg ctggaagatc
    15481 gtgcagccgc cggtgagccg cggcaccgtc tggatgagcc cggcgcacgg ccggttctgc
    15541 atggcgatgg caaggcggac cagcgtctcg cccgacatca cactgtctgc atccagtacg
    15601 acgaagctgt cgtacgcacc gccgaaccgc tgtacccagt cggcgacatt gccggccttc
    15661 ttcgcggtat tctgcaggcg ccggcggtaa tagacggcga tcgtgccatc gagccggtcg
    15721 gccagcgccg agaaaacctc ctcctcacgc aatcccgctt cgaggctgcg gctgtcgctc
    15781 aagatgaaaa cgtcgaaatt ctgcgcccgg cccatttcgc gcaggtccat ggccatcgcc
    15841 tcgacggtac ctgctattcg cgcgcagtcc tcatgataga cgggaaacag cagcgccgtt
    15901 ctatggtaga gcggctcgct cgtcgccggg atctccaagg tatcgggccg ctcccccgca
    15961 aacagcggca tgaggccgat agcggcggag agcgagccca atgcgatcca gccgaatgct
    16021 gccgtcgata gcaccaggaa gacgatctga agcggcgtga tctggacgaa cgacagcacc
    16081 gcgtagagct cgcgggcaaa cccggcggtc aacagcaagg tcccggctat gagtgcggca
    16141 cgcgtcgccc aatggacctg cgtctcggcc tgagccctcg gacacggcgg cgcaccgaag
    16201 tcctgcgccg gcatgtcgag gggctgctca gggggcagga aggagcggcc agcatcgcgc
    16261 tcggtcctca tctgagctgc ccggcgggta ctcgtcttgc tcatggtcgt ttcaacgtgt
    16321 ccagcgatag agccagcttt ccgatatgac ccgttccagg tttttgagaa cgagccgcaa
    16381 ttcgatgaga tccgccgacc cggggtcgag ctggaagctg cacctcaccc cgttgatcat
    16441 gggattgcga cggatcacga cgttcgacac cgttccagcg ctcgcagaga catcagcaac
    16501 cggaagatcg gccagttcct gcagcgccgg gccatcgaag tccaaaacga acagaaccgt
    16561 gcctttgcgc ccgttgctgc cgcgccgcgt ctttcggacg cgggctcccg cccaagccgc
    16621 cggaaccgac tcaccccagt agagccggta ggcaaaggcg aagggcttgc ccgcagggat
    16681 cggccgtccg ggcttccagt aggcgacgat gttgtcgtgg atctcctcgt ccgccgggat
    16741 ctcgatgagc tcgacgtagc cctcgcccca atctcccgca ggctccaccc aggccgtggg
    16801 gcggcgctcg tagtgcgcat cgagatcctc gtagctgctg aaggcgcggt cgcgctgcac
    16861 cagaccgaat cccttcggat ccttgcctac gaatgcgctc acttgaagaa gtcttggatt
    16921 ggtcagcggg cgccagattc gctcgccatt gccgttcagt atcgcgagtc cctcgctgtc
    16981 atgcacggcc ggacggaaat cgtttggcgt acgtcgatgc gcaggtccgt ggatgaacat
    17041 actcgtgagc ggggctagcc ccacatgcgg catctcgctc cgcggataga gcgttgcttg
    17101 cacgtccatg atcgtggaag ggccccgctc gatcgtgaac cggtaagctc cggtggtcga
    17161 agggctgtcg agcagggcgt ggacgacgat gtgcggtgcg ccgggctccg gcttctcgat
    17221 ccagaagcta cgaaaaaagg gaaactcttc accaggcggc ttggctgtgt tgatcgcaag
    17281 cccgcgcgcg gagaggccgt aaacctcttc ccggcccacg gcgcgaaaat agctggcccc
    17341 ctggaagacg acatattcgt ccatcatgtc cgcccgattg atcgggccgt gaatacggaa
    17401 gccagagaag ccgaacggcg cctcctgggg tggatcgggg attcgcggtc cgatcgagaa
    17461 cagcgtgccg tcggccttga gctggcgcgg aatgccctgc tccacgagcc agatctcgac
    17521 cggcacgtcg tagagatacc cgagcgcgaa gagctgtatc tcgtagtcga gccgctctcc
    17581 tcgccagata gcctgctccg gacggaaacg gatgtcccgg tactgttcag aggtcagctt
    17641 gtcgaacggc tccggcagct ccatcgccgg tcgctggaac ggccgctccg acagctccat
    17701 ggcgatcttc tttacgtcgt cgggagaaaa cggctgccga ctgtccgccg gctgggcggg
    17761 gccttgcgcc tttgcccaat ccgctcgcat ggcgagcgag atcacaccgg cggccagtgc
    17821 ccatgcgcca gcgtcccgga gaaccgtacg gcggttcacc ccacgattgg ccatttcacc
    17881 tgcagtcccg actctttggt agttgctgtc ggatcacgcg ttcacatcac ctctgcccaa
    17941 gggcacaacc ttcccgtgct acggacacgt gcttgcgccg cagggcttca gccaactcgt
    18001 tgaactcatg ccgattctgc cgaccccata tcgacgcatc cggtgccaaa tcaatgtggc
    18061 ggttttgctg acgaatgtgg tccagcgaaa cggggcagcc atgccgctgg gcatgtctca
    18121 gtttcgtcac cgccacaaat ccaagagtcc gcctgattgg cccgtgatcg ggtccgttat
    18181 cggcagcgga acctttggaa tttcatccaa ggcctgtgac cgctccgctt cagttgcccg
    18241 tttgaggtcc cagtcctcct gaccgcgcgg ataggctccg acgacgatga gatcggaggt
    18301 ggcgctgcgc cggcagtgcc cgaccccggc gggaatgacg accgcatcac ctgcttcaag
    18361 ggtcaggatg ggaccgctgg cgccgccgaa ctgaacctcc gcccgtcctg cggcgatccc
    18421 aaggacctcg tgcgagcgcg cgtggtagtg gtggaaggga tatattccgt cgacccacgt
    18481 gccgccccaa tcatgccggg cgaacaggtc cttgaagcgc ctttcattgt cgctccgggc
    18541 aagccctccg aagacagagc gatagacgac gagaggcaag cggtcattgt tcggaatagt
    18601 gccatcatct ctgatcaggt atgtctcggt tcggagcgtc attgttcaag gcccgtacgt
    18661 tgcgatcacc cggcatctat gtcatcaggc gccgctggtt cgcccgggcg ggccgggatt
    18721 gcgtcacggg gtggggaatg ccgatcgagg agcagacact tatcgctgcc atcatggacg
    18781 tccgcggaca ggtagacttt ctctggcagt tcttcatgac cgtgcagatc gccattttcg
    18841 cgctgctgtt catttatgac gaagcggtcg agcagctgaa ttcgttcgcg aagtttctgg
    18901 cgatcggtgc ggtggcgctg ttcgactgga tcaattccga ggcgctcaag ggcgcctaca
    18961 cattgctcga tgctctgcac cggcagtacc gggcggattt cggccagccg gaacggttcc
    19021 aatcggtgct ttacgatcat tttgtgctcg caaacttcga gggccgcgtc acgatgatca
    19081 ccatcacgca tagcctcgca ttcggtgtcg ttctgatggc tttgctctgg cgccgcttca
    19141 tccagaatgg agagccgaag aacaaggccg caaccggggc ctgaagcagg cctttgctta
    19201 caacaatgtg ttaggctcac ggttccgcgc gcgagttgca ggtcttgtcg cgcactacct
    19261 ttgaacactg ttcaggttaa ccaattttaa cttatgctcc gccccgcgct ggcaaaagtt
    19321 ggggggagcc atggcgctga agaggacaag ggcacgttgc gagacgccgg aagctttggc
    19381 cgtgaggttc gtccaagcaa tggatcgggc gagaaacgcg gctctcgtca aacgatcctt
    19441 ccagaagctc atcgagcaac ttgggttttc attcgcgatc tgcgccagac tgtcagttgg
    19501 tcagtccctc gagccactgt ccgctctgat gtgcacttgg cccgcggaat tcgcacaagc
    19561 ttaccaggcc cgcgagtatt ggcggcacga cccgatcctt caggagatca ttcgcattcg
    19621 ttgcgcggtg gattggccca aggtgttcga gcgccggcgc ttgtcacccg aggagaacaa
    19681 ggtattggag cacgcggccc aacacggcat gcgggctggt ctggccatac ccatcacggg
    19741 cgcaggcggc aatgtcggct tcgtcagcct ttgcggcccg caaccgcgat gtgatgcgac
    19801 atgccgaagc gtgctcacgc tggtctcgat ctacgtcttt caccggcttc gcgctctggc
    19861 gggggcaaac gggaccggcc agcgtctcag ccgccgcgag ctggaggtcc tgcgctggat
    19921 cgccgagggc aagtccgact ggcagatcgg ccgcatcctc gccatctcgg cgaagacggt
    19981 gaattaccac accgaaaacg tgaagcgaaa attcggcgtc gccacgcgga tgcaagccgt
    20041 ggtgagtgcc attcagcgcg gcgagctgac ccggtgacgc cagtggaatg gaagggagga
    20101 tcggcgatcc tccccgaaag gcagctgtcg atcctccccg aggggcagct aatgaaaggc
    20161 tgggtccggc taatcacttc gctggcacgg taggacgtgt gggctatgct ggccctgctg
    20221 ttccgattgc tcggatgccg tcagacgctc ttgcgcgagt tcgcacgtca ttccactttt
    20281 gggtgaggag aggtgcatcg ctcctgacgt gagctgatca cgccgacttc gacatctgct
    20341 cacgcaattt ccggtggcgc tccatcgcct cgctctcgcc gaagtcttcg agctcgtaca
    20401 cggggcgaat ctcgatctcg ctcggaccgg gcatgggatt ggggcagcgc ttcacccatt
    20461 caacggcctc atccatgtcc ttgacttgcc agatccagta gccggcgacg agctccttcg
    20521 tttctgcaaa ggggccgtcg atgacggtgc ggttcgcgcc gtcgaaggcc accctcttgc
    20581 cctgcgatga cggcttgagg ccatcgccgc ccagcatgat gccggccttg accagctcct
    20641 cgttgtactt gcccatcgcc tcgatgagct cggtcgaggg catgatgccc gcctcgctgt
    20701 ccgccgtcgc cttgacgaat accaacacgc gcataggtca tctcctctca ttggccgagg
    20761 cccattcgcc tcctgctctt acgacgaacg agtggcagcg gaaccgacac cgccccaaag
    20821 ctttttcctt agttcatggg tgcacaatgt ccggaatcgc acctacccaa ggccggcagt
    20881 gcaggaattc gctgcattga cgtgcatttg gtttaggccc acagatgccc tacgactctg
    20941 tcttgagagg ttagtcccat ggtgcgtcag cattctgttc agcgtgagag tgttggggga
    21001 gcgctcgaga accgcttgtt cgcgacgcgg caactcactc tcgagcttgc tgcaccgttg
    21061 agtgcggagg acatgaccgt ccaggccatg gatgatgcga gccccaccaa atggcatctc
    21121 gcacacgtga cctggttctt cgagaccctt attctcgccg agcatctgcc cggatatcac
    21181 ctgttcgacg aggccttcgc ctactgcttc aattcctatt acgagagcct tggtccacgg
    21241 cagccgcgcc ccaggcgagg cattctcaca cggccgtcga gcgagcgcgt gcttgcttac
    21301 cggatgcatg tcgacgaggc gctttcgaca cttttcgctc gcgatatccc gtccgatccc
    21361 cgcgtttccc acttgatcga actcggcatc aatcatgagc agcaacacca ggagctgttg
    21421 ctgaccgaca tcttggcgct tttcgctgcc aacccgctgc gcccggccta ctgtccgagg
    21481 aaggaacgcg cgccacggcc ggagccggat gctttgggct ggatcgacta cccgggcggc
    21541 atccgtgagg tcggccatgc cggcgacggc ttcgcgtggg acaacgagac gccgcgccac
    21601 aaggttttca ttcagccgtt ccggctggcg gatcgcctcg tcaccaatgc cgagtggctt
    21661 gcgttcatgg acgacggagg atacgagacc ccttcgctct ggctctcgga cggatgggcc
    21721 acggtcaatc gcgagggttg gaaggctcca tgctattggg agcagcgcga cggcgagtgg
    21781 ctggccatgt cgctggaggg cctgcatccc atagacccca gcgcgcccgt ctgtcatgtc
    21841 agctacttcg aggccgacgc gttcgcgcgc tgggcgggga agcgtctgcc cacggagttc
    21901 gaatgggaaa tcgcggcgag cggcttaccc gttcgcggca acacgctgga aacgcggtca
    21961 tttcgcacct tgccagctga accgccgaac ggccagccgc gtcagatgtt cggcgacgtg
    22021 tgggagtgga cccagagcgc ctacctcccc taccccggct atcggccacc tgccggtgcg
    22081 cttggcgagt acaacggcaa gttcatggtg agccagcagg tgctgcgcgg cgcctcctgc
    22141 gcaacgccac cgggccacac ccgcgcgacc taccgcaatt tcttctatcc gcatcagcgc
    22201 tggcagttca tgggcctgcg gctcgcctcg gagctcagct gatgctgggt caacgcgaag
    22261 atcggtcgtc ggagcacttg gccggcggca acgattttcg ggccagcgtg ctcgaagggc
    22321 tttccaggcc gcacaagtcg ttatcgagcc gcttccttta tgacgcaaag ggaagtgagc
    22381 tgttcgagga aatcacgcgg ctgccggagt actacccgac gcgcacggaa accgcgattc
    22441 tggaagcgca cgccgcagag atcgtcgacg gcgtgcctga cgggagcgtg ctcgtcgagt
    22501 tcggctcagg ctcgagctgc aaaacggaaa tcctgctcgg aaaattgccg aggctcagcg
    22561 cctatgtggc catagatgtt tcgcagagcg ccttgagagc cgccaagctg cggctagcct
    22621 cgcactttcc tcacctcgag atctatccga tcctcggtga tttttcggac cgtctcactc
    22681 ttcctgccag catcgcaaac cggagaaaga taggcttttt tccgggctcg acgatcggaa
    22741 atttcgagcc tgccgaagcc gttcggttat tgaaccattt ccggaccatc ctgtcgcccg
    22801 gcggacggct ggtcgtgggg gtcgacctca aaaaggatcc cgcaatgctc gtccgcgcct
    22861 acgatgacgc atgcggcgtt acggccgcct tcgatctcaa cctgctggcg cgcatcaacc
    22921 gtgaggtggg cggcaccttc gatctcgcgt cgttcaggca caaggccatc tacaatgcgg
    22981 acgaaggccg gatcgaaatg catctcgaaa gcaagcggga tcaggatgtc cgcgtatcgg
    23041 atcgcgtttt ccacttccgg gccggcgaga cgatccacac ggaaaacgcc tacaagtacg
    23101 caatcgctga attccgggat ctggcgcgcg ccgcacaatg gcaaccccgg cgggtgtgga
    23161 cggataagaa caatctattc agcgtgcatg agctgatcgc tgcttgaccg ggactcccac
    23221 caaaggcacg cgtctcggga cgcgtcgcca aatggcttcg agccgccgat gccgggtcgg
    23281 cagcagggac acaccgcccc aagtctccgc gcctccccct tgaacgcacc aaggggtccc
    23341 gactaactat aggccaccag gccgggcccc tctggcagct caggtgagct gtgatgtcgg
    23401 actggacatc atcggaagcg gcctgacgtg atgatctcct gccagctcca cgttgctgac
    23461 cgccgcatga ggtcgacgct tggccgctcc tacgtttcca tcgagaccaa gggcgagcca
    23521 aaatggagtt tcttgccgat tatctgacca ccggggcgct gacggccttc ggccaggtcg
    23581 ttttcatcga cctcgtgctg gctggcgaca acgccattgt catcggcctc gcagcagccg
    23641 ggcttcccgc agggcagcgc aacaaggcca tcctggtcgg catcatggcc gcgacaatcc
    23701 tgcgcatcgg gttcgcggcc attacggtgc agctgctggc tatcgtcggg ctgctcctcg
    23761 ccggcggact cttgctgctg tgggtctgct ggaagatgtg gcgcgagttg cgcgtctcgg
    23821 ccgaggttgc gcactccggc caggaagcct tggcgaatgc ggatctggac aagagcgggg
    23881 gtatcgccga aggcgccccg acgaaaacat tcgctcaggc cgcgctgcag attctcatcg
    23941 ctgacgtaac gatgtcgctc gacaatgtgc tggccgtcgc gggggcttca cgtgagtacc
    24001 cgctggtgct cgtgttcggt ctggtgctct ccattgcgct gatgggcgtg gcggcggcct
    24061 acatcgccaa gctcctgcac cggcatcgct ggatcgcctt cgtcggcctg gccgtcatcc
    24121 tctacgtggc cctcgacatg atctggcgcg gcgcaaacga cgtgtggccg catatcgaag
    24181 cggcgatcat gtaagcggcg gcgaaggagg cagggtgagc ggcggatcct tcttcggcca
    24241 cctcacccct cccgccgcgc gaactccggc aactggaacc gccgccggta ggcgcccggt
    24301 gtcaggcccg tcgtgcgctt gaacagccgg cggaagaagg cggcgtcctc gtagccgact
    24361 ttccagctga tctcgtcagc ggaggcatcg gtgcgctcga gacggcgctt ggcatcctcg
    24421 atgcgcaggc gttggacata ctcgatgggc gcaaggcccg tcgcctggcg gaagcgccgc
    24481 ttgaacgtcc gctcggacaa gccagagcgg cgaaccatct cctcgacggg gctggcgacg
    24541 gagaaatggg tcgcgagcca ttcctgagcg gcggcaatcg cggcgtcgcc atggtccttc
    24601 cggccctcga agacaatgaa cggggccaat ccatcctgat gccattgcag cgcaaacgag
    24661 cgcgcaatcg cctgcgccgc cgtcgcgccg acataccgga cgatgagata gagaacgagg
    24721 tcgtgccacg tcatcgacgc gccggacgtg atcagctcct cacgctctcc ggagacgacc
    24781 agaacgcgtt cgggctgtag agggatgttc gggaagaccc tcgcgaacgc atcggcatag
    24841 ccccaatgga tcgtcgtgtt acggccgtcg aacagaccgg tctcagcgag caggaaaaca
    24901 cctgagcagg ccgagcagag caccgcgccc cgcgcatgca tccggctcac ccagtcgacc
    24961 aactccgggt agcggccgac ctgccagcca tcagggccga gcagaatcga cggcacgatc
    25021 acgatgtccg tcgcctcgat ctccgcggcc gcgcgagggg cctcgacggg tacgccgctc
    25081 gccagcgaaa cgctgcccgc gcgggttgag acgatctcca ctttgaatgg cggctcggtg
    25141 ggaagggagg ggtcgactcg gccgagcatg cggaacgcgc ccagcacgtc gtagataccc
    25201 gtgagagtcg agacgaccgc atcagggatc gccacgagac tgacgtgcaa tggccggttc
    25261 tgccccggca tgggtccgct ccctggcacg aatgaaccgc ttttgaccag cctgcctctg
    25321 accgatcgtg ttccgagccg tcatgttccc ggccacttcg gcgcatgggg cgctggacgc
    25381 ggaacggaga gtacgatgac ggtcaatcag caaaagctca atatgcttgt cgagcgcctt
    25441 ttcggggatc tttcagcggg atatgggggc gtgatggtaa gcatcggcga caagctcggg
    25501 ctctataagg ccatggccgg cgccggtccc ctgagctcgc aggaggtggc gaggcgcgcc
    25561 ggctgcgccg agcggtacgt gcgcgagtgg ctcaattcgc aggttgccgg cgggtacatc
    25621 gattatcacc cgacatcggc gacgtacgag ctgacgcccg agcaggcggc cgtgttggcc
    25681 gacgactcga gcccggtgtt cctgccgcac gcctggcagg tagtggcgtc catgtgggct
    25741 gatgagccga agtcgcgtgc cgccatcaag accggccggg gcatgagctg gggcgagcat
    25801 gatgagcggc tgttctgcgg ggtcgctgcg ttctaccgca atgcctacag cgccagcttg
    25861 gtgagtgagt ggctgccggc gctgaccggt gtcaccgaga agctcgagaa gggcgccatg
    25921 gtggccgacg tcggttgcgg acacggacac tcgaccgtgc tgatggccaa ggccttcccc
    25981 aagagccgct tctggggctt cgatgcccac gaggggtcgg ttgcagcagc ccaagagatc
    26041 gcgcgcgagg ccggcgttgg ggaccgtgtg accttcgagg tggcgaaggc cgacacatac
    26101 cccgctcgcg gctacgatct gatctgcttc ttcgactgcc tgcacgatat gggtcggccg
    26161 gtcgacgccg tgcggcacgc gatgaaagcg gtggccaggg acggtactct catgctgatc
    26221 gagcccttcg cccgcgatag ggtcgaggag aacatcaatc ccatcggccg gctctactac
    26281 gctgcctcga cgacgatgtg ctgcgcccac gccatatccg agaacggcac ccacgtgctg
    26341 ggcgcgcagg ctggccagcg gcgcctcgag gacgtgctca aggaggccgg cctcaagacg
    26401 atccgcaagg ccgtgg
//
LOCUS       DASVDU010000269        28727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_978461, whole genome shotgun sequence.
ACCESSION   DASVDU010000269 DASVDU010000000
VERSION     DASVDU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28727
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978461"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF226_18090"
     CDS             <1..819
                     /locus_tag="VF226_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7075952.1"
                     /translation="LALILGGLLGAVLVTSTTANADQLADIKQKGTLVVGVKTDYPPW
                     GMRDANGNIVGMEPDMAAEVAKRLGVKLELVPVVSSNRMQFLQQGKIDLMIATMSVTP
                     ERRKAVGIVDPLYYAAGVALLTNKDANIKSVDDLKGKPVCALQGAYYNNDMQSKYTQT
                     DLVAFKGIPEAEQALLDGRCVGFIYDDVVLVWKKAQDKQKWANFDVLPLPQWPVVPWG
                     LAVRSEELDGPWGKTMSEMVTDWLKSGFLLELEKKWVGENTQWLKDASAKAKAN"
     gene            967..1716
                     /locus_tag="VF226_18095"
     CDS             967..1716
                     /locus_tag="VF226_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7075953.1"
                     /translation="MMETLWDALRSLHDAGGINLVQFYNDFERDRLLRGMWTTVELAT
                     ICIVLSVVIGVVGAWLQGSRNRLLKSIVQGYIQFFRNTPPLVQMYFFYFALGPQLPRI
                     VNEYGIAQPMLGSFEWAVISLSFFAGAFNVEIFRSGIEAVQRATVEAADSLGFNRLQI
                     YRYVVLPLAVRFCLPALNNNLVNLAKTTSQAYAIAVPEALFVASQIWSDHINVLEMMI
                     TLLTFYLLLVGVIVWLMTWLERSLAVPGFGR"
     gene            1731..2573
                     /locus_tag="VF226_18100"
     CDS             1731..2573
                     /locus_tag="VF226_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7075954.1"
                     /translation="MPQPARGESGFTATAFNRRSLAFVLAAFLLLVAGSEALAQIRTT
                     GGSGSIIDALVKWTPLLLLGPPGEFGGFVLNIAVSFLAMAIGTVAGLVLGLGQISLFP
                     IVRKISWAITQFFRNSPWLVLLFFVMLLTPFQIRVAGVTIPLPDWIKATVGLALPIMA
                     NMAEIVRGAVNSIPTAQWECAESLAFTRMQTLWRIILPQCLKRMIPPWMNWYAILTMA
                     TPLISIVGVNDAMTLAQDALAAEQRTELLMPMYGMLLVFFFIYCYPIARWTIYLERKY
                     AVKI"
     gene            2586..3371
                     /locus_tag="VF226_18105"
     CDS             2586..3371
                     /locus_tag="VF226_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7075955.1"
                     /translation="MPLATTRATTLAANGWTPDQPIISVRDVHKSFGKVEVLKGVTFD
                     VRKGEVVCIIGPSGSGKSTLLRCINALVPIDSGSIKVEGQEVHDPALDKLELRKKVGM
                     VFQSYNLFPHKTALENIMMAPVLVLKRPRDEVEAQARRLMKKVRLEGKENAYPGELSG
                     GQQQRVAIARSLAMNPDVMLFDEVTAALDPETVKEVLVTIRELAEEGMTCILVTHEMG
                     FAREIGDHIYFTDRGVIVEHGPPATFFNEASDPRTREFLSQIL"
     gene            3431..4738
                     /locus_tag="VF226_18110"
     CDS             3431..4738
                     /locus_tag="VF226_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7075956.1"
                     /translation="MQRSTTTEDQALWERAERHLVRYGHFFQPAIIAAAHGSFMTTTD
                     GRRLLDFASGQMSTILGHSHPEITEVIRTQAGELVHLYSQLLSRPVIDLAEALAKIAP
                     GNLERVMLLSTGGESNEAALRMAKTASGRYEVLALSRSWHGVTHGAASVTYNSNRFGH
                     GPAAPGSFVLPAPTPYRPAFTRDGKYDWEAELDYGFELFDRQSSGNPAALIIEPILSS
                     GGVIVPPAGYLRAAAERARQRGMLVIVDEAQTGLGRTGRLFDCEYDGIVPDFITLSKT
                     LGAGLALSAVLTTPEIEQKCFENKFHFYTTHASDPLPAAVGLKVIEIILRDRLADRAR
                     IAGERLRSGFETLMQRFECIGDVRGRGLLMGVELVKDRHSKAPAPELAQRVMNRCLDL
                     GLSTSIIRGGWGVFRIAPPITITDDEIDLGLSIFEQAFKDCAG"
     gene            complement(4801..5946)
                     /locus_tag="VF226_18115"
     CDS             complement(4801..5946)
                     /locus_tag="VF226_18115"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075957.1"
                     /translation="MTRRVFFYVQHLLGVGHLFRAVRVAHGLKEAGFEVDLVMGGVPV
                     ADLDFDGLNVTQLLPLKAGPGGFSDLVTMTGESAGPEIRARRITELLSAFARSRPDII
                     LIEAFPFGRRQMRFELMPLLEAAHRRDPRPRVFSSVRDILQESRQPQRVQGTIETLRR
                     WFDGIIVHGDPSIVRLDETFPIPDDLKKIVFYSGMVGPPPLLSQRNSPSAAVVVSAGG
                     GAVGRRLLETAIAAKPLTSLASERWLVLTGPNLADRDFNEIRKFGERNAITVRRFVPD
                     LAATLASAELSISQAGYNTVADVLSARCRAVFVPFSEAGETEQARRASLLQAHGFGVK
                     LDEAALSPASLAAAAEQALALPPPSLRIELNGAARTGALLASLLAET"
     gene            complement(5943..7214)
                     /locus_tag="VF226_18120"
     CDS             complement(5943..7214)
                     /locus_tag="VF226_18120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7075958.1"
                     /translation="MGSGLIIVVVKGYPRLSETFIAQELHGLERAGFRLQIASLRLPT
                     DGARHPVHQAIDAPVLYLPEYLYYAPLRVMRALIRSARLPGFWQAFWTWLADLRRDFS
                     PNRFRRFGQAAVLASEMPPEAEWLHAHFIHTPSAVTRYCSLMTGLPWSCSAHAKDIWT
                     SPDWELRDNLKSAQWTVTCTRLGYERLADLAADRAVVRLVYHGLDLRTFGRNPTPASL
                     RDGSDASDPVRLLTVGRAVEKKGIDDLLDALARLRPSLHWSLTHIGGGNLLKQLKRRA
                     RKLGIARRIVWLGPQPQSAVLVAYRSADIFVLPCRTARNGDRDGLPNVLMEAQSQRLV
                     CLSTKVGGVPELIRHGETGLLVDPNDCAALAKALDYLIRNPEVRERLGRAGEKHVREH
                     FDVQAGLGELCKLFQSSLALKHSQQSVEAEA"
     gene            complement(7229..8407)
                     /locus_tag="VF226_18125"
     CDS             complement(7229..8407)
                     /locus_tag="VF226_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075959.1"
                     /translation="MYSHDTFGLGHLRRCREIAHALVERYKGLHVLIISGSSIAGAFD
                     FRARVDFVKIPSVIKLHNGEYTSLADHIDLAETLQMRRAIIRQTAELYRPNIFIVDKE
                     PLGLRGELEQTLVMLKSLGATLVLGLREVLDSPEQVRAEWQRSDALRKIEALYDAVWV
                     YGCERFWDPLTGLDVPRSVRCRMTFTGHLGRSAPRLQNPTRQELPKDYILVTAGGGGD
                     GGPLMQQVLAAREYDRSLEFPLVMVLGPFMSSETRAEIHARAKRVPNVTVLDFERQIE
                     SIVLGARAVVSMGGYNTFCEILSFDKRSLLVPRSQPRREQLIRATRAAELGVVDVLEP
                     ALADDPQVMARALRRLPTRPLPSALGTPIDLGGLESVQEMVGAYLDGSVHSLKTSVAM
                     "
     gene            complement(8613..9791)
                     /locus_tag="VF226_18130"
     CDS             complement(8613..9791)
                     /locus_tag="VF226_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018209807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075960.1"
                     /translation="MTSMLLPPPGSPLQHYVSEKPFDPKSIDRLSAAQERIYLASQWR
                     LMWWKFKRHRVAVVSGIFLGILYISILFCELLAPYHYQSRHIDHIYSPPQRVHLFHEG
                     QLIGPFVYGQKYSLNMETLKRQYEPDRSVVHPIRFFCRGDPYNFWGIFESDVHLFCPA
                     ENGTLFLLGTDRLGRDVLSRIIYGARVSLTIGLIGVAISFMLGLIFGGLAGYYGGIID
                     LVIQRIIEVLQSLPTIPLWLALAAIMPVTWSPMVVYFAITVILGLIDWTGLARAVRSK
                     LLSLREEDYVLAAQLMGASPVRIIGRHLVPGFMSHLIASATLAIPSMILGETALSFLG
                     LGLRPPITSWGVLLTEAQNINIVALYPWLLTPAIPVILVVLAFNFLGDGLRDAADPYK
                     "
     gene            complement(9788..10786)
                     /locus_tag="VF226_18135"
     CDS             complement(9788..10786)
                     /locus_tag="VF226_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003595069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7075961.1"
                     /translation="MLLYIFRRILIMIPTLLLTSALVFTIMELPPGDYFESYMAELQA
                     QGEGVDKARIEFLKKEYGFDKSPVERYFIWLGGLLQGDLGYSFEYQMPVSELVGSRIF
                     LTVLVSFATILLTWLIAFPIGIYSATHQYSWGDYGLTFLGLMGIAVPNFLLALILMYF
                     ANVWFGTSIGHLMAPEYLGQPMSWAKFKSILEHMWIPVIVIGLAGTASMIRKIRANLL
                     DELQKQYTITAKAKGLHPFKALVKYPLRSSLNFFVGGIGGILPEIVSGAEIVAIVLSL
                     ETTGPLLIKALQSQDMYLAGSFLMFLALLTVIGVLISDIALAFLDPRIRLQGRSTR"
     gene            complement(10799..12730)
                     /locus_tag="VF226_18140"
     CDS             complement(10799..12730)
                     /locus_tag="VF226_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003492295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7075962.1"
                     /translation="MTNAIDRRRFNCILLSAPALGVAGYLPASARDFIEPPYLKDQIE
                     AKRLPPLQERIPVKPRVIDLERMERKPGRYGGQIRMLMGDPKDVRMVVVYGYARLVGY
                     DENLNLVPDILENFEVEDGRIFTLRIRPGHRWSDGHPFTAEDFRYFWEDIANNEKLSL
                     TGPPAEMLVDGKPPVFEVLDECTVRYTWPAPNPAFIPALAGALPLYIYAPSHYLKQFH
                     VKYAHPAELELKVTSARVRDWTSLHERMGRMYRPQNPDLPCLDAWCLSTAPPSTRFVF
                     KRNPYFHRIDTEGRQLPYADEIILNIGSSALVAAQTATGEADLQARYIRFDDYTFLKS
                     AEKRGGFNVELWGRGEGSRMALLPNLNAEDPVWRKLWRDVRVRRALSLAIDRREINQA
                     IFYGLGRESANTMLPQSPLHRLEYQTAWTRFDLDQANKLLDEAGLAKRDWDGIRYLPD
                     GRRAELVVETAGESTEQADVLALIRDNWLKIGIKIYTRPTQRDLFRKRVYAGSVVMSV
                     WSGLDNALATPDMSPHELAPTGQAQLQWPKWGQHYEEKGAVGEPPDLPEARRLLELYL
                     AWRQSRSTAERAVIWHEMLKIHADQVFTIGTVNGTRQPVVVSKRLVNVPEEGIWAFDP
                     GGYFGRYLPDTFWFRDTAE"
     gene            complement(12727..14640)
                     /locus_tag="VF226_18145"
     CDS             complement(12727..14640)
                     /locus_tag="VF226_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7075963.1"
                     /translation="MSDLLRIEDLRISFSLHGIWHEVVKGVSFRVRRGSVVALVGESG
                     SGKSVIAQSIMGILPQVGAITGGRILLASEGNEKPADLAALDPDGPEMRKLRGTAMSM
                     IFQEPMTSLAPLHTIGNQISEALTTHFPVGKKEARARSEEMLGLVGFPDPKRAFDMYP
                     FELSGGLRQRAMIAMALICRPALLIADEPTTALDVTIQAQVLSLLKELQQRLNMGMLL
                     ITHDLGVVANMADEVIVIYHGEIMEAGTADAIFRTPRHPYLKALLKAVPHFDMSPGER
                     LISLRGSETKKKASPPAPPAVRLRPPSKGDAPLLKARNLKKSFETRKQGWFGRSGVEK
                     IAAVDGVSFDVRRGECLGLVGESGCGKSTLSKILMRALTPDSGTLVYTGPGGESVDLL
                     KLEGSALKAFRPRIQMVFQDPVSSLSPRMTVLNIIREPLQIHNRGSAREQIDRCVELM
                     EAVGLDPRFLNRYPHSFSGGQRQRIGIARALVLNPDMLILDEPVSALDVSVQAQILNL
                     LKDLKAELGLTYLFISHNLAVVDYMADRIAVMARGRLVETAPRHILFRKPVHPYTRAL
                     LNAVPFPDLDRPLDFKLLNIGGASDEASWPPQFRVNGEGARLEYIDLGGGHRVLANPN
                     ADAGELEAVEVGV"
     gene            complement(15198..16499)
                     /locus_tag="VF226_18150"
     CDS             complement(15198..16499)
                     /locus_tag="VF226_18150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7075964.1"
                     /translation="MLQHEPIWWLDAPPQSLPHGPVDKRSDVVIIGGGYTGLSAALTL
                     ARSGRSVQVFDRMRPGEGASTRNGGIASGNLRSGYRELAAKFGQERAGSILAEAKAAR
                     EYLETFIRNEGIACDLAMTGRFTGAATPADYEALARDADILRRTFGIEAHAVPPAEQR
                     RVLGTDFYEGGVLRMDIGGLHPGKLHRELLRLVLEAGVKVHGETAVSGVRSDGEAFEV
                     ETVRGRVRATHVITGTNGYTDGVDPWLRRRLVPVRSRIIATAPLSGNLMRQLMPQGVM
                     CSETRKLHYYYRPSPDGARILFGGRDGSIAGDPQWPVDNLRRALADIFPELEGVEITH
                     TWFGHVAMNRDGVPRIFSHKGVRYAAGYCGSGVVWANWAGRKAALQIIGEEDGKSALD
                     FRPPPPIPLYNGRPWFLPVVFGWKTAQDRLAAQRRKRARKA"
     gene            16703..17065
                     /locus_tag="VF226_18155"
     CDS             16703..17065
                     /locus_tag="VF226_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075965.1"
                     /translation="MLKIAALAAASFGLAVSAHAQAPNQGQQMCQQIWSKSDTQKQGF
                     VTGQQAMRLNQAIRTAQATPGTSGKRGATGGAAGGTTQGMPKSGSAGGGMQGQRVTQQ
                     EFMSACQRNPQAFQHLRS"
     gene            complement(17126..17536)
                     /locus_tag="VF226_18160"
     CDS             complement(17126..17536)
                     /locus_tag="VF226_18160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075966.1"
                     /translation="MVESPPGVTAVCVTVAQLEDVVSAITRAPSTDADTLQGCVKAII
                     DVLIAHGLEEQDELIETGSAICARLIAFAALLKSAEIGPWIASVGVQASSLQRPLLQA
                     MATAPLQVVQGHFRFDPKDFLDIALENTPPAGNG"
     gene            17704..18033
                     /locus_tag="VF226_18165"
     CDS             17704..18033
                     /locus_tag="VF226_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075967.1"
                     /translation="MNDNGKQTGGQSGRGESARDLVAQSMYQLVRLARLGIQAAASGM
                     ERLEESMARKQRERAETRPMGEAPPTTGTTRTSAAEEPRPPPGHADDQMGGSNRPSEH
                     DSGQSKH"
     gene            18152..18892
                     /locus_tag="VF226_18170"
     CDS             18152..18892
                     /locus_tag="VF226_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075968.1"
                     /translation="MKSEDPEVLRVKRKGRGSLLCRRVEFDAGTAGVSQSEQLVCFLP
                     WLLSYGNCRKARLLPPNLLAAYEMPHGIVSSSPETCVAALQVVVDDFLELMLERSLQP
                     AKLTLLGLSIGNFAATYIANMIGARLWAVAPGDRGEVLLWNSSIARGLRSQAEARGFG
                     YADFEATLKAFNPVNNLRHIGAGSTFIVGRFDTVVPYRSAMNVATEAKANNPTARNLV
                     LPLGHSSTLFTGVQLLRFNLWNRKCRAA"
     gene            19349..19693
                     /locus_tag="VF226_18175"
     CDS             19349..19693
                     /locus_tag="VF226_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075969.1"
                     /translation="MRDLYAQGDLLIERVDEVQPSGTLSAANSDGSFVLAEGELTGHH
                     HSIFGNVTMFRDDSLARDIPGGLYIGHINVDGPAARVQHQEHAPLTLPKGTYRVRRQR
                     ELEPKDARVVAD"
     gene            19680..20786
                     /locus_tag="VF226_18180"
     CDS             19680..20786
                     /locus_tag="VF226_18180"
                     /inference="COORDINATES: protein motif:HMM:NF042824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075970.1"
                     /translation="MSQTNSRTRLERLSPLQTQEVAEYRSRWAAIGVETRPADRDAAE
                     DGVRIAYRAAGLPSPKIVWCEGPLEFAHEWRNAVRSAIGQSVKREIYDHPLARVITYA
                     ESRVSLGVRHTVINGTRLPAATSLAVNEVVHAAVEDARPRLWTRLKQSWRMRRIARFR
                     DSGWGHPDFAWLAACDYLASLCGTADADALEGILKIAKNSGWIVPHEHVCWLGERPSI
                     LRVDDKARLHSREGPALQYRDGLSIYMWKGVQVPDWMIEQPNLITPSFIDRHPDFLLR
                     RCMIEIMTPAQYVASGGAIPIARDETGILWRKQWWNNDAWAAVEVVNGTAEPDGTYRH
                     YFLQVPPNVRSPREAVAWTYGLTETEYTRLRLRT"
     gene            complement(20840..21403)
                     /locus_tag="VF226_18185"
     CDS             complement(20840..21403)
                     /locus_tag="VF226_18185"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEX7075971.1"
                     /translation="MSLGRNFLQSITFCEEMKRKITQNARFPWRRYVLAAVVVGLTFL
                     PAHARNAQYSQNEKTVVGVLTCTAFEGHDERSATERLAISCRFQAKAGGYTEDYVGTL
                     RRLAGKLPLEKPIALMWTVSADPAVLSPGVLGQSYLSSAPADAPESRQLQGQKNESIV
                     LSPFSGNGNKAEHTVTVLDLDLKRTGA"
     gene            21588..21809
                     /locus_tag="VF226_18190"
     CDS             21588..21809
                     /locus_tag="VF226_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075972.1"
                     /translation="MELRTLAYGLAAFLISAVLMTLLGLGDAAGNTTGLLFWALLAIL
                     AIAYAVSRFRNGEHEPVRVARWLKHRDGR"
     gene            21948..22244
                     /locus_tag="VF226_18195"
     CDS             21948..22244
                     /locus_tag="VF226_18195"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HEX7075973.1"
                     /translation="MADPFTRNDPTAIPGASPQRTAEAGESLGVKDTADYQAEIEKLR
                     SDMTRLAQSIGGTISDSMKPITRDLEAAVARNPTVSVAIAAGVGLVLGFLMSRR"
     gene            22558..22968
                     /locus_tag="VF226_18200"
     CDS             22558..22968
                     /locus_tag="VF226_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX7075974.1"
                     /translation="MVEPKGTIPASDRGFAGMDPAKQRDIARKGGESVPAAKRSFAQN
                     PQLASMAGRKGGASVPAAKRSFSQNHALASQAGRKGGESVPREKRSFSQNPELASQAG
                     RKGGESVPPEKRSFSQNRGLAAEAGRKGGENRSR"
     gene            23268..24137
                     /locus_tag="VF226_18205"
     CDS             23268..24137
                     /locus_tag="VF226_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX7075975.1"
                     /translation="MPRPTWKGYLKLSLVSCPVALYPATTTRSRISFHIMNRKTGDRT
                     RYLVVDSETGEPVEPEDRVRGFEISKNNYVFIEDEEIDAVKIESTHTIDIDSFVPRKE
                     VDEIYLDSPYYLAPDGKVGEDAFLVIREAMRQKQMVGIARVVMSRRERMLLIEPRGKG
                     LLATTLRNADEVRSEAAYFEGISDMELPEEMLAIAKDIIARMSGKFDPSKFEDRYEKA
                     LKELIEAKRTGAPVHATEAPEPRPTPNLMDALRRSLELGPPAPRARGRRAQAATQAAR
                     RRGNGKAAARRAS"
     gene            24134..26623
                     /gene="ligD"
                     /locus_tag="VF226_18210"
     CDS             24134..26623
                     /gene="ligD"
                     /locus_tag="VF226_18210"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEX7075976.1"
                     /translation="MSADLRDYAAKRDFSRTREPRGRKGESTRTDLQFVVQKHAARRL
                     HYDLRLELDGVLKSWAVTKGPSLVPGEKRLAVHVEDHPLEYGDFEGRIPEGEYGAGEV
                     IVWDRGTWHPEGDPHKGYAKGHLDFTLKGAKLKGRWHLVRMRRRAGEKQEGWLLIKSD
                     DDAARSPGDPDILEEQPESVISGRTIEDIAEQETDLPDFIEPCLAKLADSPPSGSKWV
                     HEVKFDGYRIQVRKSGDKVELLTRKGLDWTEKFGSLPKAFNRISARSAVIDGELVVEE
                     ASGASSFAALQAALKEGRFGELRYYAFDLLFRDGADLRDRPLLERKKQLSEILAGLPE
                     ASPIRLSEHFETEGATLLKHACRMGLEGIVSKRIDKPYRSGRGTDWIKTKCVERREFV
                     VIGFSPSTTTRRAIGALAIGDYDRGKLRYRGRVGTGFTEETSRMLFQLLDPLRRSQPP
                     LEEIPAGERRRGIRWVEPKVVVEVEFRTWTEAGLVRHAVYGGLREDKPAEEVTAEKPA
                     RSKTADPPAHDFDESKLTHPERLLWPDAGITKLGLAEYYADIWQWIAPHIVGRPLSLV
                     RCPEGIGQSCFFQKHAWAGIDSAIRRFRLEGEDEDSLYIEDLDGLIGLVQASVLEIHP
                     WGSTVADVERADRMIFDLDPGPGLSWGDVIDAASELRDLLRETGNLQSFVKLTGGKGL
                     HVVVPLRPAADWDAVKAYSQSVAEHMARRNPDRFTASIAMRTRNGRIFVDYLRNGRGA
                     TAVAAYSTRARAGAPVAAPVEWNELRAGVGPAAYTLINLPARIRHLDRDPWAGIGKIE
                     QTLPEPMEKGSARAAGRQVKATKRKPRNTGT"
     gene            26746..27120
                     /locus_tag="VF226_18215"
     CDS             26746..27120
                     /locus_tag="VF226_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7075977.1"
                     /translation="MFESEITVMTPRQFAVLAALDGLEGASQAELTKHTGIDRSTLSE
                     VVQRILAKGFVVRRRSTTDRRAYTVKLTRSGRSKLSAVRPAVERVDEQILSMIPARQR
                     DAFVTALTALTEACEKRMRGEE"
     gene            27402..27989
                     /locus_tag="VF226_18220"
     CDS             27402..27989
                     /locus_tag="VF226_18220"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7075978.1"
                     /translation="MPMRRNIFDPLQDIKRELDQVFDRFLGEGFAPSPAQTGEMGMIS
                     PNVDVCVEGDTLRIDADLPGVEPEDVECTLKEGVLTIKGERRPKRAGEGDGSRRRERK
                     FGRFERVIELPEGVDEESMEARFDKGVLTITAHMKPGFGRPKRIRIAGKGASQGTGTQ
                     QQSATAQPGSSQQTQSDKPDQGAQAGQQTRPANGQ"
     gene            complement(28044..28223)
                     /locus_tag="VF226_18225"
     CDS             complement(28044..28223)
                     /locus_tag="VF226_18225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075979.1"
                     /translation="MANHSPHHDDEERAVQRTPVEARQGSRNRANLRVLILSFGIAVL
                     VLAILYLSFFPSSYE"
     gene            complement(28323..>28727)
                     /locus_tag="VF226_18230"
     CDS             complement(28323..>28727)
                     /locus_tag="VF226_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7075980.1"
                     /translation="GEEIARQVATDALIIEDEPLIVMDLTQILEELGHRVIAVARTRD
                     EARKAIAKQRPGLVLADIQLADGSSGLDAVNDLLGSFEVPVIFITAYPEKLLTGEKPE
                     PTFLITKPYQPETLRVTISQALFFDQRSRRAA"
BASE COUNT         5883 a         8798 c         8790 g         5256 t
ORIGIN      
        1 cttgcactta ttcttggcgg cctgctcggt gccgtgctgg tgacgagcac caccgccaac
       61 gctgaccagc ttgcggacat caagcagaag ggcacgttgg ttgtcggtgt aaagaccgat
      121 tatcctccct ggggcatgcg agacgcgaat ggaaatatcg tcggcatgga acccgacatg
      181 gcggctgaag tcgccaagcg cctcggtgtc aagctggaac tcgtgcccgt cgtctcatcg
      241 aaccgcatgc agttcctgca gcaaggcaaa atcgacctga tgatcgccac gatgagcgtg
      301 acgcctgagc ggcgcaaggc cgtcggcatc gtcgatccac tctactacgc cgcgggcgtg
      361 gcgctgctca ccaacaagga tgcgaacatc aagtccgtcg acgacctgaa gggcaagccg
      421 gtctgtgccc tacagggggc ctattacaac aacgacatgc aatcgaagta cacgcagacg
      481 gacctggtcg ccttcaaggg aattcccgag gccgagcagg ctctgctcga tggccgttgc
      541 gtgggattca tctatgacga cgtcgtgctt gtctggaaaa aggcccagga caagcagaag
      601 tgggcaaatt tcgatgttct cccgcttccg caatggccgg tggtgccgtg gggtctcgcc
      661 gttcggagcg aggagctcga tggtccctgg ggcaagacca tgtcggaaat ggtgaccgac
      721 tggctgaagt ccggcttcct tctcgaactc gagaagaaat gggtcggcga aaatacgcag
      781 tggctgaagg acgcctccgc gaaagccaag gcaaactgac caagacttcg ggccgcagtg
      841 aatgggcaga tctgccccgc tgcggccccg taattctcgg gcttcaagac gtgccggggc
      901 gccgcccttt cgacggaggg gacgcatcga tttcagggca ctcatcagaa gcacccagac
      961 ggaagaatga tggaaaccct gtgggacgcg ctgcgtagcc tgcatgatgc cggcggcatc
     1021 aatctcgtac agttctacaa tgatttcgag cgcgaccggc tgctgcgggg catgtggacc
     1081 acggtcgagc tggccaccat ctgcattgtt ctaagcgttg tcattggggt cgtcggggct
     1141 tggctgcagg gcagccgcaa tcgcctgctc aagtcgatcg tacagggtta cattcagttc
     1201 ttccgcaaca cgccgccgct cgtgcagatg tattttttct attttgcact cggcccgcag
     1261 ctcccccgga tcgtcaacga gtatggaatc gctcagccga tgctcggcag cttcgagtgg
     1321 gcggtcatat cgctgtcatt ctttgccggt gctttcaatg tcgaaatttt ccggtcggga
     1381 atcgaggccg ttcagcgggc cacggtcgaa gcggccgact ctctcgggtt caacaggctg
     1441 cagatctatc gctacgtcgt cctcccgctc gccgtgcgct tttgcctgcc ggccctgaac
     1501 aacaatctgg tcaacctcgc gaagacgacg agccaggcct atgccatcgc cgttcccgag
     1561 gcgctgttcg tcgccagtca gatctggtcg gatcacatca acgtgctcga gatgatgatc
     1621 acgctgctga ccttctacct cctgctcgtc ggcgtgatcg tctggctcat gacgtggctc
     1681 gaaaggtctc tggccgtccc agggttcggc cgatgagcgc gggcttacag atgcctcagc
     1741 cagcccgggg tgaaagcggg ttcaccgcga cagccttcaa tcggcgctcg ctggcgttcg
     1801 ttctcgccgc atttctcctg ctcgtcgccg gcagcgaggc cttggcccag atcaggacca
     1861 cggggggtag cggcagcatc atcgatgcgc tggtcaagtg gacgccgctg ctgctgctcg
     1921 ggccgccagg cgaattcggc gggttcgtgc tcaatattgc ggtgagcttc ctcgccatgg
     1981 ccatcgggac ggttgcgggg ctcgtcctgg gacttggcca gatctcgctc ttccccatcg
     2041 tgcgcaaaat ttcttgggcg atcacacaat tcttccgcaa ctcgccctgg ctggtcctgc
     2101 tgttcttcgt gatgctgctg acgccgtttc agatcagggt tgccggcgtg acgatcccgt
     2161 tgccggattg gatcaaagcc acggttggac tggccctgcc gatcatggcc aacatggcgg
     2221 agatcgtcag gggagccgtc aattccatcc cgaccgcaca gtgggaatgt gccgaaagcc
     2281 tcgccttcac ccggatgcaa accctctggc gcatcatcct gccccaatgc ctgaagcgga
     2341 tgatcccgcc ctggatgaac tggtacgcca tactcaccat ggcgaccccg ctgatctcga
     2401 tcgtcggtgt caatgacgcg atgaccctgg cgcaggacgc actcgccgcc gaacagcgga
     2461 ccgagttgct gatgccgatg tatggtatgc tcctggtttt ctttttcatc tactgctacc
     2521 cgatcgcgcg ctggaccatt tacctggagc gcaaatacgc cgtgaagatc tgaggagtcc
     2581 gtccgatgcc gctcgccacg acccgcgcca ctacactggc cgcgaacggc tggacaccgg
     2641 atcagccgat catctccgtt cgtgacgtgc acaagtcgtt cggcaaggtg gaggtgctga
     2701 aaggcgtcac attcgacgtg cgcaagggcg aggtcgtatg cattatcggc ccgtccggct
     2761 ccggaaaatc gacgcttctc cgctgcatca acgcgctcgt gccgatcgat agcgggtcga
     2821 tcaaggtcga gggccaggag gtgcatgatc cagcgctcga caagctggag ctgcggaaaa
     2881 aagtcgggat ggtttttcag tcctacaatc tttttccgca caagaccgcg ctcgagaaca
     2941 tcatgatggc gccggtgctc gtcctcaagc gcccgaggga tgaagtcgaa gcccaggccc
     3001 ggcgcctgat gaaaaaggta cggctcgaag gcaaggagaa cgcttacccg ggcgagctgt
     3061 cgggcggcca gcagcagcgg gtcgccattg cgcggtcgct cgcgatgaac ccggacgtca
     3121 tgctgttcga cgaggtgacg gcggccctcg atccggagac ggtgaaagaa gtgctggtta
     3181 cgatccgcga gcttgccgaa gaaggtatga cctgcattct ggtcacccat gaaatgggct
     3241 tcgcccgcga aataggcgat cacatctact tcacggaccg cggcgtcatc gtggagcacg
     3301 ggccacctgc cacgttcttc aacgaagcgt ccgatccgcg cactcgcgag tttctgagcc
     3361 aaatcctatg agcaatttca actggacctg acctcacagg cccgcggcac aggctgaagg
     3421 atcacgcacg atgcaaagga gcacgaccac cgaggatcag gcgctttggg agcgggccga
     3481 acgccatctc gttcgctacg gtcatttctt tcaaccggcc atcatcgcgg cggcgcacgg
     3541 ctcgttcatg acgacgacgg acggccgacg gcttctggat ttcgcctcgg ggcagatgag
     3601 caccattctc ggccatagcc accccgagat cacggaggtt atccgcaccc aggcgggcga
     3661 gctcgtccat ctctacagcc agctcctgtc acgcccagtg atcgatctcg ccgaagcgct
     3721 ggcgaaaatc gctccgggca atctcgagcg ggtcatgctg ctctcgacgg ggggagagtc
     3781 caacgaagca gccctgcgca tggccaagac ggcgagcggt cgctacgagg tgctggcgct
     3841 gtcccgctcc tggcatggcg tgacgcacgg ggctgcgtcc gtcacctaca acagcaaccg
     3901 ctttggtcac gggcccgccg cgccgggctc cttcgtgctg cccgcgccga cgccctacag
     3961 gccggctttc acgcgcgacg gcaagtatga ttgggaggcc gagctcgact atggcttcga
     4021 gcttttcgac cggcagtcga gcggcaatcc agcagcgctc atcatcgagc ccattctttc
     4081 atccggcggc gtgatcgtgc caccggctgg ttacctgaga gccgccgcgg agcgcgctcg
     4141 ccagcgtggg atgctggtca tcgtcgacga agctcagaca ggattgggcc gaaccggtcg
     4201 gctgttcgat tgcgagtacg acggcatcgt gccggacttc atcacccttt ccaagacgct
     4261 cggagccggt ctcgcccttt cggccgttct gacgacccct gaaatcgagc aaaaatgctt
     4321 cgagaataaa tttcatttct acacgaccca tgcgtccgat cccctccccg ccgctgtcgg
     4381 gctgaaggtg atcgaaatca ttctgcgcga ccggctcgcc gatcgtgcac ggatcgctgg
     4441 tgagcggctg cgcagcggct tcgagacgct gatgcagcgc ttcgaatgca ttggcgacgt
     4501 acgcggtcgc ggcctgctga tgggcgtcga gctggtgaag gaccggcaca gcaaagcgcc
     4561 ggccccggag ctcgcgcagc gtgtcatgaa ccgctgcctc gacctcggcc tgtcgacgag
     4621 catcatccgc ggtggctggg gtgtgttccg gatcgcgccg ccgatcacga ttacggacga
     4681 cgaaatcgac ctgggccttt ctattttcga gcaggccttc aaggattgcg caggatagat
     4741 gcaggataga ttctatactg tgtccagctt tgcaaccata cgtattggga cagctgccta
     4801 ctaggtctca gccaacaggc tggcgaggag cgcccctgta cgtgcggctc cgtttagctc
     4861 aatgcgcaag gagggcggcg gaagtgcgag agcctgctcg gccgcggccg ccagggaagc
     4921 aggtgacagc gcagcttcat ccagcttcac tccgaagccg tgcgcctgga gcagactggc
     4981 acggcgggcc tgctcggttt cgccggcttc agaaaacggc acgaaaaccg cccggcaccg
     5041 agccgaaagg acgtcggcga ccgtgttgta tccggcttgt gaaatcgaga gttcggcact
     5101 cgccaaggtc gccgcaagat ccggaacgaa gcgccgaacc gtaatagcgt tcctctctcc
     5161 aaatttgcga atctcgttga aatcgcgatc ggccaaattc gggccggtga ggacaagcca
     5221 acgttccgat gcaagtgaag tgaggggctt ggcggcaatt gccgtctcga gcagcctgcg
     5281 cccaaccgct ccgccgcctg ctgaaacgac gacggctgca gatgggctgt ttctttgcga
     5341 cagcagcggc ggaggtccga ccatgccgga gtagaaaacg atctttttaa gatcatcggg
     5401 aatagggaat gtctcatcaa gccgcacgat gctggggtcc ccgtgcacaa tgattccgtc
     5461 gaaccaccgt cttagggttt cgatcgttcc ctggacccgc tgcggctgcc ggctctcttg
     5521 gagaatatct cggacagagg agaatacgcg gggtcgcgga tctctcctgt gggcggcctc
     5581 caatagcggc atcaattcaa aacgcatttg ccgccggccg aacggaaatg cctcgatcag
     5641 aatgatgtcc ggccggctgc gggcgaaagc cgagaggagc tcagttatcc ggcgcgctct
     5701 tatttcggga ccagcggact cgcccgtcat cgtcaccaag tcgctgaagc ctcccggtcc
     5761 ggctttgagc ggcagaagct gagtgacatt cagcccgtcg aaatcgagat ccgcaacagg
     5821 cacgccgccc atcactaggt ccacctcgaa accggcttcc ttaagtccgt gcgcgacgcg
     5881 gacggctcga aagagatgcc ccacgccgag gaggtgctgc acgtaaaaga agacgcgtcg
     5941 cgtcacgctt cggcttccac tgattgctgg ctgtgcttta aagcaagtga ggactggaac
     6001 agcttgcaca attccccaag gcccgcctgc acatcgaaat gctctcgtac gtgcttttct
     6061 ccagcgcgcc ccagccgttc ccgcacctcc ggattccgga tcagataatc caacgctttc
     6121 gcgagagcag cacaatcatt cggatccacc agcaggccgg tttccccgtg gcggatcagc
     6181 tccggaacgc cgccgacctt cgtcgatagg cacaccagcc gctggctctg ggcctccatc
     6241 agcacgttcg gcaagccgtc ccgatcgccg ttacgcgctg tgcggcaggg caacacaaaa
     6301 atgtcggctg aacgataggc tacgagcacg gcggattgcg gctgagggcc aagccagaca
     6361 atgcggcgcg caatcccaag cttccgcgct cgtctcttga gctgtttcag cagattgcct
     6421 ccgccgatat gcgtcaagct ccaatggaga gagggccgaa gccgcgccaa cgcgtcgagc
     6481 aggtcgtcga tacccttttt ttcaactgcg cgtccgaccg tcagcagacg aacgggatcg
     6541 gatgcgtcgc tgccatctct cagtgaggct ggcgtcgggt ttctaccgaa tgttcgaaga
     6601 tcgagcccgt gataaacgag cctaacaacg gcccgatccg cggcgagatc cgcaagacgt
     6661 tcgtagccga gccgcgtgca agtgaccgtc cattgtgccg atttgagatt gtcgcgtaat
     6721 tcccaatccg gcgaggtcca gatatcctta gcgtgtgccg agcagctcca cggaaggccc
     6781 gtcatcaggc tgcagtagcg cgtcaccgcc gatggcgtgt gaatgaagtg cgcatgaagc
     6841 cactccgctt ccggtggcat ttccgaagcc agaacggcgg cctggccaaa ccggcgaaag
     6901 cgattggggc tgaaatcgcg gcgaaggtcg gcaagccagg tccaaaacgc ttgccaaaag
     6961 cctggcaggc gcgccgagcg gataagggca cgcatgacgc gcaggggcgc gtaataaagg
     7021 tattcgggaa ggtaaagaac aggagcgtca atcgcctggt ggacgggatg acgagcgcca
     7081 tccgtcggaa gacgcagcga ggcgatctgc agccggaagc ctgcgcgctc cagcccatgc
     7141 aattcctgtg cgatgaacgt ttcggataac cgggggtagc cctttacgac gacgatgatc
     7201 aatccggacc ccacttgagc aggctccgtc acatcgcaac agaggttttc agagaatgga
     7261 cgctgccgtc caaataagcg cccaccattt cctgcacact ctccagaccc cctaggtcaa
     7321 tgggtgtgcc aagcgcggag ggcaaaggcc gggtcggcag gcggcgcaga gcgcgcgcca
     7381 tcacctgcgg atcgtccgcc aaggctggct ccagcacgtc gacgactccc agctcggcag
     7441 cgcgcgtggc ccggatcaac tgctcgcgcc ggggctggct gcgcggaacc agcagcgaac
     7501 gcttatcgaa cgagaggatt tcgcaaaacg tgttgtaacc acccatgctg acgacagcgc
     7561 gcgcgccgag gacaattgac tcgatctgcc tttcgaagtc gagaaccgtg acattgggga
     7621 cgcgcttcgc tcgcgcgtgg atctccgctc gcgtttccga tgacatgaac ggcccgagaa
     7681 ccatgaccaa tggaaactcg agcgagcgat catattcgcg ggccgccaga acctgctgca
     7741 tcaatggccc gccatcgcca ccgccgccgg ccgtgacgag aatatagtcc tttgggagct
     7801 cttgccgtgt cggattctga agccgaggtg cgctgcgccc gagatggccc gtgaatgtca
     7861 tccggcatcg cacggaccgc ggcacatcca aaccggtgag cgggtcccag aagcgctcgc
     7921 agccataaac ccagacagca tcgtaaaggg cttcgatttt ccgcagggca tcactgcgct
     7981 gccattccgc ccgcacctgc tccggggaat ccagcacctc gcgcagacct agcacaagcg
     8041 tcgcaccaag cgacttgagc atgacgaggg tctgctccaa ctcacccctg aggcccaatg
     8101 gttccttgtc gacgatgaaa atattgggcc gatagagctc cgcagtctgc cggataatcg
     8161 cacgtcgcat ctgcagcgtt tcggcaagat caatgtgatc tgccaaggaa gtatattcgc
     8221 cgttgtgtaa cttgatgacg cttgggatct ttacgaaatc gacgcgcgca cggaaatcaa
     8281 aggcaccggc aatggacgag ccggatatga ttaagacatg cagcccctta tatcgttcga
     8341 cgagcgcatg agcgatttcc cggcaccgtc gcaggtgacc gagcccgaac gtgtcgtggc
     8401 tgtacatgag caagcgtgca tcgccatgtc gtagcataga cctgccgcga gcaaaccact
     8461 cctcgtccgg ccccgatccc aataactcgg ttgccaggcc agcgttccgg ctcggagctt
     8521 gctcccctgc tgcctctggt ctgcaacatt cggttgcaaa accgctcaac cgtcaaccga
     8581 aatctcttcc gaaaaggtaa ccggatcgtt ggtcacttat agggatcggc cgcatcacgc
     8641 aggccgtccc ctaagaagtt gaacgcgagg acgacgagaa ttaccgggat cgccggcgtt
     8701 agcagccagg gataaagcgc cacgatattt atgttttgcg cttcggtgag cagaacgccc
     8761 cagctcgtaa ttggaggacg cagcccaaga ccgagaaagc tcagcgccgt ctcgccgagg
     8821 atcatgctcg ggatggcaag cgttgcactt gcaatcaggt ggctcataaa gcccggcacg
     8881 agatgccgtc cgatgatccg gaccggactc gcccccatca gttgtgccgc gagcacataa
     8941 tcttcttctc gcaacgagag cagtttggat ctgaccgccc gggcaaggcc cgtccagtcg
     9001 atgagcccaa gaatcactgt gatagcaaaa tagaccacca tagggctcca ggtgaccggc
     9061 atgatggccg caagcgccag ccacaggggg atcgtgggca atgattgcag gacttcgatg
     9121 atcctctgga tgacgagatc gataatgcct ccgtaatatc ctgccagccc accgaaaatg
     9181 agcccgagca tgaaactgat tgcgacaccg atcaggccaa tggttaacga gacgcgcgcg
     9241 ccataaatga tgcgagacag cacgtcccgc ccaagccggt ccgttccgag caggaacagc
     9301 gtcccgttct cggcggggca gaaaaggtga acgtcgcttt cgaagatgcc ccagaaatta
     9361 tagggatcac cacggcagaa gaagcggatc ggatgaacaa cggaacggtc cggttcatac
     9421 tgccgcttca gcgtctccat attgagagag tatttctgcc catagacgaa cggccctatc
     9481 aactgtcctt cgtgaaaaag gtgaacacgc tgcgggggtg aataaatatg gtcgatgtgg
     9541 cgcgactgat agtgataggg cgccaggagc tcgcagaaga gtatagagat gtaaaggatg
     9601 ccaagaaaaa tccccgaaac gacggcgacg cggtgccgct tgaacttcca ccacatcagc
     9661 cgccattgcg aggcgagata gatgcgttcc tgcgccgcgg ataggcggtc gatcgacttc
     9721 ggatcgaacg gcttttcgga gacgtagtgc tgcaatggcg aacccggtgg cggcaggagc
     9781 atgctcgtca tcgcgtgctc ctaccctgca gtcgaatgcg tggatcgaga aacgccaaag
     9841 caatgtcgga aatcagcacc ccgatgaccg tcagcagcgc cagaaacatc aggaacgagc
     9901 cggcgagata catatcctga ctctgcaggg ccttgatgag aagcggtccg gtcgtctcga
     9961 gcgatagcac gatggcgacg atctcggcgc ccgagacgat ctccggcagg atccctccga
    10021 tgccgccgac aaagaagttg agggatgaac ggagagggta cttcacgagt gccttgaagg
    10081 gatgcagacc cttggccttg gcagtgatcg tgtactgctt ctgcagctcg tcgagcaagt
    10141 tggcgcggat cttccggatc atgctggccg tgccggcaag cccgatcacg atcaccggga
    10201 tccacatgtg ctccaggata gacttgaact ttgcccaact catcggttgg ccgagatatt
    10261 ccggcgccat caagtgcccg atggaggttc caaaccacac atttgcaaag tacatgagga
    10321 tgagcgccag aagaaagttt ggcaccgcaa tccccatcaa gccgaggaac gtcaggccat
    10381 agtcgcccca gctatactga tgagtggccg aatagatgcc gatcgggaac gcaatgagcc
    10441 atgtcagcag gatcgtggcg aacgagacca gaacagtgag gaaaatgcgg ctgccgacga
    10501 gctccgaaac gggcatctgg tattcgaatg aatatccgag atctccctgc agaaggcctc
    10561 caagccagat gaaatagcgc tcgaccggag acttatcgaa gccgtattcc ttcttgagaa
    10621 actcgattct cgccttgtcg acgccctctc cctgggcctg cagctcggcc atgtagcttt
    10681 cgaagtagtc gccagggggc agctccatga tcgtaaacac gagcgcgctg gtgagcagca
    10741 gcgtcggaat catgatcaga atgcgacgga aaatgtagag gagcatggat aagctcggct
    10801 actctgcggt gtctcgaaac cagaaggtat caggcaggta ccggccgaaa tagccacccg
    10861 gatcaaaagc ccaaattcct tcttcaggca cattgacaag ccgctttgaa acgacgaccg
    10921 gctgccgagt tccgttaacg gttccgatgg taaagacctg atcagcgtga atcttcagca
    10981 tctcgtgcca tatgaccgcc cgttccgccg tcgagcgcga ctggcgccac gccagataga
    11041 gttccagcag ccggcgcgct tctggcaggt ccggcggctc cccgacggcg cctttttctt
    11101 cataatgctg accccatttc ggccactgga gctgcgcctg ccccgtcgga gctaactcgt
    11161 gcgggctcat gtccggagtc gccagcgcat tatcgaggcc ggaccagacg gacatgacga
    11221 cgctccctgc ataaacgcgc ttacggaaca ggtctcgttg tgtcgggcgc gtgtaaatct
    11281 taattccgat tttcagccag ttgtcacgga tcaatgcaag aacgtcggcc tgctcggtgc
    11341 tttcgcccgc cgtctcgacg accagttctg ctcgccggcc atcgggcaga taccggatac
    11401 cgtcccaatc ccgcttggcc aggcccgcct catcgagcag cttgttcgcc tggtccagat
    11461 cgaagcgtgt ccaagccgtt tggtattcca gccggtgcaa cgggctttgg ggcaacatcg
    11521 tgttggcact ctcccgcccg agaccataaa agatggcctg attgatttct cgccgatcga
    11581 tcgcgagcga cagggcccgg cggacccgca cgtcccgcca aagttttctc caaacggggt
    11641 cctccgcatt caaattgggc agcagcgcca tgcgcgagcc ctcgccgcgt ccccaaagct
    11701 cgacgttgaa cccgccgcgc ttctcagcgc ttttcaggaa cgtatagtcg tcgaagcgga
    11761 tatagcgcgc ctgaaggtcc gcctccccgg tcgctgtctg tgccgcgacg agcgcgctcg
    11821 agccgatgtt aaggatgatc tcgtctgcat agggcagctg ccggccctcg gtgtcaatgc
    11881 ggtggaagta cggattgcgc ttgaagacaa accgtgtgct gggcggcgcc gtactgagac
    11941 accaggcatc caggcatggc aaatcggggt tctgtgggcg gtacatgcgg cccatgcgtt
    12001 catgcagcga ggtccagtcg cgcacgcggg cagacgttac tttcagctcg agctctgccg
    12061 gatgggcgta tttgacatgg aactgtttca gataatggct tggcgcatag atatagagcg
    12121 gcagcgcacc ggccagcgcg gggatgaaag ccgggttggg cgcaggccat gtgtagcgca
    12181 cagtgcattc atcgagcacc tcgaaaactg gaggcttacc atcgaccagc atttccgcag
    12241 gcggaccggt caatgacagc ttctcattgt tggcaatgtc ctcccaaaaa taccggaagt
    12301 cctccgcggt gaaaggatgc ccgtcggacc agcggtgacc aggcctgatc cgcagcgtga
    12361 agattcggcc atcttcgacc tcgaaattct ccagaatgtc gggcacgagg ttcaggtttt
    12421 catcgtatcc gaccagacgc gcatagccgt aaacgacgac catgcgaaca tccttcggat
    12481 cccccatgag catcctgatc tggcccccgt accgacccgg cttccgctcc atgcgttcaa
    12541 gatctatgac gcggggcttg accggtatgc gctcttgcag cggaggcagc cgcttcgctt
    12601 cgatctgatc tttcaggtat ggcggttcga taaaatcgcg ggcggatgcc ggaagatatc
    12661 cagccactcc cagcgcagga gccgacagca gaatgcagtt gaaacgcctc ctatcgatcg
    12721 cgttcgtcac acacccacct cgactgcttc gagctccccg gcgtcggcat tcgggttcgc
    12781 caacacacga tggcctccgc cgaggtcgat atattcgagg cgcgcgcctt cgccatttac
    12841 ccggaactgc ggcggccagc tcgcctcgtc cgaagcgcct ccgatgttga gaagcttgaa
    12901 atcgagcggc cgatcgaggt cagggaaggg cacagcattc aacaatgccc gcgtataggg
    12961 atgcacaggc ttacggaaca ggatatgacg cggcgcagtt tcgaccaaac ggccgcgcgc
    13021 catcacggct atgcggtctg ccatgtagtc caccacggca aggttatggg agatgaacag
    13081 gtaggtcagc cccagttccg ccttcaggtc tttcaataga ttcaagatct gcgcctgtac
    13141 ggagacatcg agcgccgata cgggctcgtc caggatgagc atatcgggat tgagcaccag
    13201 cgcacgcgca atgcctatcc tctgccgctg accgccggaa aagctatgcg gatagcggtt
    13261 caggaagcgt ggatcgagcc ccacggcctc catcagctcc acacaccgat cgatctgctc
    13321 ccgggcgctc ccgcggttat gaatttggag cggctcccga atgatgttga ggacggtcat
    13381 gcgcggcgaa agggagctga cggggtcctg gaacaccatc tgaatgcgcg ggcggaacgc
    13441 cttgagagcc gacccttcga gtttcagaag atcaaccgac tcgccgccgg gacctgtata
    13501 aacgagagtg ccggaatcgg gcgtgagagc gcgcatgagt atcttgctga gcgtcgactt
    13561 tccgcagccg gattccccca cgagccccag gcattccccg cgcctgacat cgaagctgac
    13621 accgtcgaca gctgctattt tctccacgcc ggaacgaccg aaccagccct gcttgcgggt
    13681 ctcgaaggat tttttcaggt ttcgcgcctt gagcagcggc gcgtcgccct tgctcggagg
    13741 cctgagccgc acagccgggg gggccggcgg ggaagccttt ttcttcgttt cggagccccg
    13801 caacgagatc aaacgctcac cggggctcat atcgaaatgc ggcaccgcct tgagcaatgc
    13861 tttcaaatag gggtgccgag gcgtgcgaaa gatcgcgtct gcggtgcccg cttccatgat
    13921 ctcgccgtga taaatcacta tgacttcgtc agccatattg gcgacaacgc ccaggtcgtg
    13981 cgtgatgagc agcatgccca tattgagcct ctgctgcagc tccttgagca ggctgagcac
    14041 ctgggcctga atggtgacgt cgagagctgt cgtcggctcg tcggcgatca acagcgccgg
    14101 tcggcagatg agcgccatag cgatcatcgc cctctggcgc agtccgccgg aaagctcgaa
    14161 cgggtacatg tcgaatgcac gcttgggatc ggggaacccg accagcccga gcatttcctc
    14221 gcttcgcgcg cgggcttcct tttttccgac cggaaaatgc gtcgtcaggg cttcggaaat
    14281 ctggttgccg atggtgtgaa gcggagccag cgacgtcata ggctcctgaa agatcatcga
    14341 catggccgtg ccgcgcaatt tgcgcatttc aggcccgtcc ggatcgaggg cggccaggtc
    14401 ggcgggcttc tcgtttccct ctgatgcaag cagaatgcgc ccgccggtaa tcgctccgac
    14461 ctgcggaagg atgcccatga tggactgggc gatgaccgat ttgcccgagc ccgactcgcc
    14521 taccagggcc acgacgctgc cgcgccgaac ccgaaagctc acgcctttga cgacctcgtg
    14581 ccaaatgcca tgcagcgaaa acgagatcct caggtcttcg atacgcagca ggtcactcat
    14641 ctgatacggc gatcgtctcg ggtgccagaa tcatcaaccg gttccgggtt atgaccccgg
    14701 catccaaaat ggcgagcgca gtcagcttgg gcgcatacag ccgatgatgc acgggtgcgg
    14761 cccctgctcg cagcagcccc ggaataagaa tcgcgatcag cgactattcc gcggcacgtt
    14821 taccatatca aagtcggaga gctttccaag acaaagccct taccgctcga cacagtacat
    14881 cattgtgaca gcatcgataa gccccgctgc ggaccatcac cgcagcaggg tttgttttaa
    14941 ccacgaacca ggctgaaaaa gaatggcgca cgtttgccgc actccgcggc aaaacttcac
    15001 ctgtggcgga atggatggtt ccgcccgacc aaaggccggt ggagatcctt tgcgcgaccc
    15061 gcacaacgag cgcgttttac cctgctgccg cggctcacct ccgcggcttt accctttttt
    15121 tcccgcatcg tttcgaatag tgggtaaaac tgcctgcggc cgctccggac ctcctgcttg
    15181 ggcgatgaat tcagaagtca ggcctttctt gcgcgcttgc gccgctgggc cgctaggcgg
    15241 tcctgtgcgg tcttccagcc gaacacgaca ggaaggaacc agggtctgcc attgtaaagc
    15301 gggatgggcg gcggcgggcg gaaatcgagg gccgatttgc catcctcttc gccgatgatc
    15361 tgcagcgctg ccttgcggcc ggcccaattc gcccagacga cgcccgagcc acaataaccg
    15421 gccgcataac ggacgccttt gtgcgaaaag atccgcggaa cgccgtcgcg attcatggcc
    15481 acgtgtccga accacgtgtg cgtgatctcg acgccctcga gctctggaaa gatgtcggcc
    15541 agtgcgcgcc gcagattgtc caccggccat tgcggatcac cggcaatcga gccgtcgcgt
    15601 ccgccgaaca gaatgcgagc accgtcgggc gagggtctat aataatagtg tagcttgcgc
    15661 gtctccgagc acatcacgcc ctgcggcatg agctggcgca tcaggttgcc ggacagcggc
    15721 gccgtcgcaa tgatgcggct acgaaccggc accagccgcc ggcgcagcca cgggtcgacg
    15781 ccatccgtgt agccattcgt tcccgtgatg acgtgtgttg cccgcacccg gccgcgcacc
    15841 gtttctactt cgaaggcctc accgtctgag cggacgccgc tgacggccgt ctccccatgc
    15901 accttgacac cggcctcgag gacgagccgg agcagctcgc gatgcagctt gcccggatgc
    15961 aagccaccga tatccatgcg gagcacgccg ccttcgtaaa agtccgtccc gaggacgcgg
    16021 cgctgctcgg cgggaggcac tgcatgagcc tcaatgccga acgtcctgcg gagtatatcg
    16081 gcatcgcgtg caagcgcttc gtaatctgcg ggggtggctg cgcccgtaaa gcgcccggtc
    16141 atcgccaggt cgcaggcaat tccttcgttt cggatgaaag tctccaggta ttcccgggcg
    16201 gccttcgctt ctgcgagaat agatccggcg cgctcctggc cgaatttcgc ggcaagctcc
    16261 cggtagcccg aacgcaggtt gccgctcgcg atgccgccgt tccgggtgga ggcgccctcg
    16321 cccggacgca tgcgatcgaa gacctgcacc gaccggccgg aacgcgccag ggtcagcgca
    16381 gcgctcagtc ccgtataccc gccgccgatg atcaccacgt ccgatctttt gtcgaccggg
    16441 ccgtgcggca gcgattgcgg tggcgcgtcg agccaccaga tcggttcatg ttgaagcact
    16501 ctggccccct gggttcaatg gcttttgtgc tgcttgcaaa gtgcaccagg caatcgccgc
    16561 cgtccagaaa agcaaggtat tgccgtcgcc tcgaggcagg agaaagcaag gaacagaact
    16621 tctcgtgttg cgttgcaggt cgtcccaaaa ctgcgcgtgc aggcgggaca aatgaaaaca
    16681 tcgaggagtt catccgatgc gaatgctcaa gatcgcagcg ctggccgctg catccttcgg
    16741 gctagcagtg tctgcccacg cccaagcccc caatcagggt cagcagatgt gtcagcaaat
    16801 ctggtcgaag tccgatactc agaagcaggg gttcgtcacc ggccagcaag ccatgcgatt
    16861 gaaccaggcc attcggacag cacaggccac acctggcaca tcgggcaaac gcggcgccac
    16921 cggcggcgca gccggcggta ccacccaagg catgcccaaa tccggctcgg ctggtggcgg
    16981 tatgcagggg cagcgcgtga cccagcaaga attcatgtcg gcatgtcagc gtaatccaca
    17041 ggcattccag cacctgagga gctgaggcgc cgtattcgcc ggcggggaag ctcgccccgc
    17101 cggtttcgcg gcaccagagc gctcctcagc cgttgccagc cgggggcgtg ttctcgagag
    17161 caatatccag aaagtctttc gggtcgaacc gaaagtgccc ctgcactacc tggagcgggg
    17221 ccgttgccat cgcctgcagc aaaggccttt ggagcgatga agcctgaaca ccgacagagg
    17281 caatccaggg accaatctcc gccgatttca gcaatgccgc gaacgcaatc aaccgcgcgc
    17341 agattgccga acccgtttca atgagctcgt cctgctcctc cagcccatgt gcgatcagga
    17401 catcgatgat cgccttgaca cagccctgaa gcgtgtccgc gtcggtggac ggcgccctgg
    17461 taattgcgct gaccacatcc tcgagctggg caaccgttac gcatacggcc gtgacgccgg
    17521 gaggagattc gaccacatgg gcctctattg ctgatcatcg tcgggcagag aacattcaac
    17581 ctttttcgtg gaaggcgggt tccaaggacg tggggcaggc aagcgcctct tccgggaacc
    17641 gtggcccagt cggggcgtta ggcggggcaa gcgcgttgag atgcccacta tgagggcccg
    17701 acaatgaacg acaacggcaa gcagacaggc ggccagtccg gccggggcga atctgcccgc
    17761 gatctcgtcg ctcaatcgat gtaccaactg gtgcgcctgg cgaggcttgg gatccaggcg
    17821 gccgcatccg gaatggagcg gctcgaggag tccatggccc gcaagcagcg tgagcgcgcc
    17881 gaaactcgcc ccatgggaga agcgccgccc acaaccggca cgacccgcac gagcgcagcg
    17941 gaagagccac gccccccgcc cgggcacgcc gatgatcaaa tgggcgggtc caatcggcca
    18001 agtgagcacg actcgggtca aagcaaacac tagagcggac ttcgaagcag aaattcgcac
    18061 cgggtcgcca tttggggcag cgaatttctc atcagataaa ggctggcttg aactggctgg
    18121 caatggacgg caattcgcag gggcacgtat cgtgaagagc gaagatccgg aagtcctccg
    18181 cgtcaagcgg aaggggcgag gcagcttgct ctgtcgccgc gtcgagttcg acgccggcac
    18241 ggcaggcgtt tcgcaatccg agcagctggt ctgctttctg ccctggttgc tgagctatgg
    18301 aaattgtcgc aaggcacggc tgttgccacc caatctgctg gccgcatacg agatgcctca
    18361 tggcatcgtg agctcatcgc ctgagacatg cgttgctgca ctgcaggtcg tcgtcgatga
    18421 ctttctggaa ttgatgctgg agcgcagcct gcaacccgcc aagctcacgt tgctgggact
    18481 gagcatcggc aatttcgcag cgacctacat cgcgaacatg atcggtgcac gcctttgggc
    18541 ggtcgcgccc ggagaccgtg gcgaggtcct gctctggaac agctcgatcg ccagggggct
    18601 gcgcagtcaa gcggaggcaa ggggcttcgg ctacgccgac ttcgaggcga ccctgaaggc
    18661 gtttaatccc gtcaacaact tgcgccacat cggcgccggt tcgactttca tcgtcgggcg
    18721 cttcgatacc gtcgttccct accggtccgc gatgaacgtg gccaccgagg ccaaggcgaa
    18781 caatccaacc gcccgcaacc tcgtgctgcc gcttggacat tccagcactc tgttcactgg
    18841 agttcagctc ctccgcttca acctgtggaa ccgaaaatgt cgcgctgctt gatcctttcc
    18901 aggcaagcgc cttccaggcg aactcgtcag caatacccag ggtgccgcgc cagcagccgc
    18961 gctcaagatt acgcccgcga gagagtgagt tccgtgaccg gcgcgccaag cacgcaagcg
    19021 cccgaatcgg gcggtgtcga cacaatccgc accagacgta aagcataaaa tcgtaagtgc
    19081 agagaaaaaa tcaacagaag actggaacca gttggcaatg ttcacgttag ttggttgcga
    19141 ccgcatcaac aaactcccta cgcccccccc ccgtggcgag agatgtggtc ggtgcagggg
    19201 gcgccctccc tcggcgcccc ctgttttttg ctgctgcaga cggagctggt ggcgtgttta
    19261 tagtggtctg tgatctccat cagtgccggg aacctaagaa agcttttgcg gttgatcctg
    19321 cagccaacag gaaactggga gccctaagat gagagaccta tacgcacaag gtgacttgtt
    19381 gatcgaacga gttgacgagg tgcagccatc cggcaccctg tccgcagcga attccgatgg
    19441 ctcgttcgtc ctggcggaag gcgagttgac cggtcaccat cattcgatct tcggcaacgt
    19501 cacgatgttc cgtgatgata gcctggcgag agacattccc ggcgggctct acatcggtca
    19561 catcaatgtc gatggtcccg ccgcgcgcgt gcagcaccag gagcatgcgc ccctgacact
    19621 gccgaaaggc acctaccgcg tccgccgtca acgcgagttg gagcccaagg acgcccgtgt
    19681 tgtcgcagac taacagccgt actcgcctgg agcggctgtc gccgctccaa acgcaggaag
    19741 tcgcggagta tcgaagccgc tgggccgcca tcggcgtaga gacgcgcccg gcggaccgcg
    19801 acgctgcgga ggacggtgtt cgaatcgcct accgcgcagc aggcttaccc tcccccaaga
    19861 tcgtctggtg cgaggggccg ctcgagtttg cgcacgaatg gcggaatgcc gtccggagcg
    19921 ccataggcca gagcgtcaag cgcgaaatct acgatcaccc cctcgcccgc gtcataacgt
    19981 atgccgagtc ccgggtcagc ctgggcgtgc gccacaccgt catcaacgga acacggcttc
    20041 ctgcggcgac gagcctcgct gtcaatgaag tggtgcacgc ggctgtcgag gatgccagac
    20101 cgcgcctgtg gacgcggctg aagcagagct ggcggatgcg ccgcatcgcc cgattccggg
    20161 acagcggatg gggtcatccg gacttcgcct ggttggcggc ctgcgattat ctcgcatccc
    20221 tctgtggcac tgccgatgcg gacgctctgg aaggcatcct gaagattgcc aagaactccg
    20281 ggtggatcgt tccccatgag catgtttgct ggctgggcga gcggccaagc atcctccgtg
    20341 tggatgacaa ggcgcggctg cacagtcgcg aggggccggc ccttcagtac cgggacgggc
    20401 tctccatcta catgtggaag ggtgtgcagg tccccgactg gatgatcgag cagccgaacc
    20461 tcatcacgcc gtcgttcata gaccggcatc ccgatttcct gttgcgccgt tgcatgatcg
    20521 agatcatgac gccggcacaa tacgttgcaa gtggcggcgc gatcccgatc gcgcgggacg
    20581 agaccggcat cctgtggcga aagcagtggt ggaacaacga cgcgtgggcc gccgtggagg
    20641 tggtcaacgg aacggcagag cctgatggca cctaccggca ttacttcctt caggtgcccc
    20701 cgaacgtccg ctctccgcgc gaggcggtgg cctggacgta cggcttgact gaaacggaat
    20761 acacgcggct tcgcctgcgt acgtagcttc ctgcggggcg cgatcgcatc gcttcctatc
    20821 tcacgccgac aatcgcgttt caggcacccg ttcgcttcag atcgagatcg agcacggtaa
    20881 cagtgtgctc ggccttattt ccgtttccgg agaaagggct cagcacgatg gactcgttct
    20941 tctggccctg caactggcgg ctttccgggg cgtccgcggg cgcgctgctc agatagctct
    21001 gccccaggac gcccggcgaa agcaccgctg ggtcggctga taccgtccac atcagcgcga
    21061 tgggcttttc cagcggcagc ttgcccgcga gccgccttag cgttcccacg taatcttccg
    21121 tgtaaccgcc tgctttggct tggaagcggc acgagatggc gagtcgctcg gtcgccgaac
    21181 gctcgtcgtg cccctcgaag gccgtgcagg tcagaacgcc cacaacggtc ttctcattct
    21241 gagaatattg agcatttcgt gcatgggcag gcaaaaacgt aagaccgact acaacggcag
    21301 caagcacata ccgtctccag ggaaaccggg cgttctgggt gatttttctt ttcatctctt
    21361 cacaaaacgt gatgctctgg agaaagtttc gccccaagga catgctgggg tgaggaacgg
    21421 atcgaagggg cacttgttct aacgaacatg aggatgcacc aatcagaact agtatgaatg
    21481 cggcggacct gagacccaat caacaacgaa gaatatagcc acataaggat tgggaaggac
    21541 gacggcgggc ggtcggggct catcgccgca agaaaggcat ggtgatcatg gagcttcgca
    21601 cactcgctta tgggttggcg gcctttctga tttcggccgt gctaatgacg ttgctcggct
    21661 tgggcgacgc ggcgggcaac actacgggtt tgctcttctg ggcgctgctg gcaattctcg
    21721 cgattgccta tgcggtatcc cgattccgga acggcgaaca tgagcccgtg cgggttgcac
    21781 gctggctgaa gcaccgtgac gggagatagg cccggctccc ttattgagtc tcggagccgt
    21841 ggcctatccc ggaactgatg caacggcttg cccgttctaa tttccaactc caactccgcg
    21901 cgagcattgt cgctcccgcg ttcagcccaa cagcgaggtg aggaacgatg gccgaccctt
    21961 tcacccggaa cgatccgacg gcaattccag gtgcgtcccc gcagcgcacc gctgaggctg
    22021 gggaatccct cggtgttaag gacacggcgg attaccaggc ggagatcgaa aaactgcgct
    22081 ccgatatgac ccgcctcgcg caatcgatcg gcggcacgat cagtgacagc atgaagccga
    22141 ttacgcggga cctcgaggca gcggtcgccc gcaatccgac cgtttccgtc gccatcgccg
    22201 caggcgtggg tctggtattg ggttttctga tgtccagaag gtgagcggca attgcgataa
    22261 gtagagcttt ttgacgtccc ggtgccgaat ggtgacggga ctttttgtgc gccacaagcc
    22321 ggttcggagg cgaggaccaa ggacgcgcgc cgggcatgcg gcaatgttga gcgagggaac
    22381 ctccgcgcgc agcctcagtt cttgttcaag aaatggcgct gaggagcatg gccgaacgcg
    22441 gcgcccctca cactaaaagc gtcgccgata gaggctggct cagcatccag ggaagagggc
    22501 ggcgaggcgg acctattccg ccgtgaagtt taccgattgc cttgggaggc attccatatg
    22561 gttgaaccaa agggcacgat tccagcatcg gatcgcggct ttgccggaat ggacccggcg
    22621 aagcagcggg atatcgcccg caaaggcggc gagagcgtgc cggccgcaaa gcgcagcttc
    22681 gcacagaacc cgcaactcgc atccatggcc gggcgcaaag gcggcgccag tgtcccggcc
    22741 gcgaaacgca gtttctcgca gaaccacgca cttgcctcac aggccgggcg caagggcggc
    22801 gaaagcgtcc cgcgagaaaa gcgcagcttc tcgcaaaatc ccgagcttgc ttcccaggcg
    22861 ggtcgcaagg gtggggagag cgtgccgccg gaaaagcgca gcttctctca gaaccgcggc
    22921 ttggccgcag aagccggccg caaaggaggc gaaaaccgct ccaggtgacg atcggtcgcc
    22981 gggctcggga gaaaattctc aaatacagga ggcagcgttt gcctcatccg aaacccaatc
    23041 cgcccaagcc tggtgacttg cccggcgaag tgcccgatcc gatgccagtt ccgcccccgg
    23101 tggaccccac gcgccagccc ggcgggccag agcgcgatcc gaccggcgag ccgaagaaac
    23161 gtggtccccg cagggacggt tagtcaagac gttcggaaag ctacggcccg tccgtgaacc
    23221 ctggcggttc tccggcgtcc atccgcagtt acgcccggag ttcgctcatg ccaagaccaa
    23281 cctggaaagg ctacctcaag ctttccctcg tctcgtgccc cgtggccctc tacccggcga
    23341 cgaccacacg cagccgaatt tcgtttcaca tcatgaatcg caagacgggc gacaggaccc
    23401 gctaccttgt cgtcgacagc gaaactggcg agccggtcga gccagaggac cgcgttcgcg
    23461 gattcgaaat cagcaagaac aactatgttt tcatcgagga cgaggagatc gatgccgtca
    23521 agatcgaaag tacccacacg atcgacatcg acagcttcgt tccgcgcaaa gaggtcgacg
    23581 aaatctatct cgattccccc tactatctgg caccggacgg caaggtcgga gaggatgcat
    23641 ttctggtcat tcgggaagcc atgcggcaga agcagatggt gggcattgcg cgggtcgtga
    23701 tgtcccgccg cgaacgcatg ctgctgatcg agccgcgcgg gaagggtctg ctggcgacga
    23761 cgttgcggaa cgccgatgag gtccgcagcg aggccgctta cttcgagggc atttccgata
    23821 tggagttgcc cgaagaaatg ctcgccattg ccaaggacat catcgcccgc atgtccggca
    23881 aattcgatcc aagcaaattc gaggatcgtt acgaaaaggc gctcaaggag ctcatcgagg
    23941 ccaagcgtac cggcgccccg gtgcacgcga ccgaggctcc cgagccgagg ccgacgccca
    24001 atctcatgga cgccctgcgc cgcagcctcg agctcgggcc acctgcccca cgggcgcgtg
    24061 gccgccgcgc gcaggctgca actcaggcgg cccggcggcg cggtaacggc aaagcggcgg
    24121 cacggagggc ttcgtgagcg ctgacctgcg cgattacgct gccaaacggg atttttccag
    24181 gacgcgcgag ccgcggggcc ggaaaggcga gtcgactcga accgacctgc agttcgtggt
    24241 tcagaaacac gcagcacggc ggctgcacta tgatttgcgg ctggagcttg atggcgttct
    24301 gaagagctgg gccgtcacaa agggcccgag cctcgtgcct ggcgaaaagc ggctcgccgt
    24361 tcacgtcgag gaccatcctc tggaatacgg cgatttcgag gggagaattc cggagggtga
    24421 atacggtgcc ggggaggtca tcgtgtggga ccgcggtacc tggcacccag aaggcgaccc
    24481 gcacaaaggc tatgccaagg gccacctcga cttcacgctc aaaggagcca agctcaaagg
    24541 tcgttggcat ctggtccgga tgcgcaggcg tgccggtgag aagcaggaag gctggctgct
    24601 gatcaagtcc gacgacgatg ccgcacgcag tcccggagat ccggacattc tcgaagagca
    24661 gcccgagtcg gtcatcagcg gcagaacgat cgaggatatc gctgagcagg aaaccgatct
    24721 gccggacttc atcgagccat gcctcgcaaa gctcgccgac agcccaccat ccggcagcaa
    24781 atgggtgcac gaggtcaaat tcgacggtta tcgcatccag gtgcgcaaga gcggcgacaa
    24841 ggtcgagctc ctgacccgga agggtctcga ttggacggag aaattcggct cccttccgaa
    24901 agccttcaat cgcatttcgg cccgatcggc cgttatcgac ggcgagctcg tcgtcgagga
    24961 ggcatccggc gcatcgagct tcgcagccct gcaggccgca ctgaaggaag gccgcttcgg
    25021 ggagctcagg tattacgcgt tcgacctgct ttttcgcgat ggagcagatc tgcgcgatcg
    25081 cccattgctg gaacgcaaga agcagctgtc ggagattctc gcgggcctcc ccgaggccag
    25141 tcccatcagg ctgagcgagc atttcgagac ggaaggcgcc actctgctca agcacgcctg
    25201 ccgcatgggg ctcgaaggca tcgtctcgaa gcggatcgac aaaccttatc gctccgggcg
    25261 gggcacggat tggatcaaaa cgaaatgcgt tgaacggcgg gagttcgtgg tgatcggctt
    25321 ttcgccgtcg accacgaccc ggcgcgccat cggcgccctc gccatcggcg actacgatcg
    25381 cggcaaactt cgctatcggg gccgggtcgg aacagggttt actgaagaga caagccgcat
    25441 gctgtttcag ctgctggatc ccttgcggcg ctctcaacct cctcttgaag aaataccggc
    25501 aggcgagcgg cggcgcggga tcagatgggt cgagcccaag gtcgtggtcg aggtcgagtt
    25561 ccggacatgg accgaagcag ggctcgtcag gcacgcggtt tacggcggat tgcgcgagga
    25621 caagcccgcc gaggaggtga ccgccgaaaa accggctcga tcgaaaacgg cggaccctcc
    25681 cgcccatgat ttcgacgaga gcaaactcac gcacccggaa cgcttgttgt ggcccgacgc
    25741 cggaatcacc aagctgggtc tggccgaata ttatgccgac atctggcaat ggattgctcc
    25801 gcatatcgtg ggacgccccc tcagcctggt gcgctgcccg gaagggatcg ggcagagctg
    25861 tttttttcag aagcacgcct gggccggcat tgattccgcc atccgccggt tccgtctgga
    25921 gggggaagat gaggatagtc tctacatcga agatctcgac ggtctcatcg gtttggtgca
    25981 ggcaagcgtt ctcgaaatcc acccctgggg cagtacggtt gcagatgtcg agcgcgctga
    26041 tcggatgatc ttcgatctcg atccgggccc cggcctttcg tggggcgatg tcatcgatgc
    26101 cgcgtcagag cttcgggatc tgctgcggga aactggaaat ctccaaagct ttgtcaaact
    26161 gactggtggc aagggattgc acgtcgtcgt cccgctccgt ccggcggcgg actgggatgc
    26221 cgtgaaagca tattcacaaa gtgtcgcgga gcacatggct cggcggaacc cggaccgctt
    26281 tactgcgtcc atagccatgc gcacgaggaa cggacgcatt ttcgtcgact atcttcgcaa
    26341 tggccgtggt gccacggcgg tcgcggcgta ctcgacgagg gctcgtgcgg gagcgcctgt
    26401 tgcggctccg gtcgagtgga acgaacttcg tgccggggtc ggaccagccg cctacacgct
    26461 gatcaatctg ccggcccgta tccggcacct cgaccgcgat ccttgggccg gaattgggaa
    26521 gatcgaacaa acactccctg aaccgatgga aaagggaagc gcacgtgcgg ctggccggca
    26581 agtcaaggct acgaaacgca agccgcgcaa tactggaacc tgatgagatc taccgggttt
    26641 cgacaattaa aatgacagtg gtgttcagaa aagatgaccc aagagaaaag cttaaaccgc
    26701 tccgtcctgc acttgctcta tagggcgatg cagtgtgcct cgaacatgtt cgagagcgaa
    26761 atcacggtga tgacgcctcg gcagttcgcg gtcttggcgg cactggacgg cctcgaaggg
    26821 gcctcccaag ccgagctgac caagcatacg ggcatcgacc ggtccacgct ttcggaggtc
    26881 gtgcagcgca tcctggccaa gggcttcgtt gtccgtcgca ggagcactac cgaccgtcgc
    26941 gcctacacgg tcaagctgac gcgctccggc cgttccaaat tgtcggccgt ccggccggcc
    27001 gtggagcggg tcgatgagca gatcctcagc atgataccag cccggcagcg ggacgcgttc
    27061 gtaacggcgc tgacggctct gaccgaggct tgtgaaaagc ggatgcgggg cgaagagtaa
    27121 gacgtctcaa gtcgcttgct cgggcgagct agcgccgacg ccggcgcgcg cccccatccg
    27181 atgagcccct cccatcgccc cgagctccat accaggcccc tctttctgat cttccagaag
    27241 agttccgggc aacctccgcc gcccttggag catttcggaa cattttcaac tcgggcgcac
    27301 aaggaggacc gggcggcgat cgggaaggtc cggccggaac attgcgctcc agacacgcgt
    27361 tccatttcgc gaaccggtcc attgtcaagg gagagtcgtc gatgcctatg cgccggaaca
    27421 ttttcgaccc gcttcaggac atcaaacggg agctcgacca ggtcttcgat cgctttctgg
    27481 gcgagggctt tgcaccgtcg cccgcgcaga cgggcgagat gggaatgatt tctcccaacg
    27541 tcgacgtctg cgtggagggc gatacccttc ggatcgatgc agacctaccc ggtgtcgagc
    27601 ccgaggatgt ggaatgcacg ctcaaggaag gcgtgctgac gatcaagggc gagcgccgcc
    27661 cgaaacgggc aggggagggg gatggaagcc gccggcgtga gcgcaagttc gggcggttcg
    27721 agcgggtaat tgagctgccc gaaggcgtcg acgaggagag catggaagcg cgcttcgaca
    27781 agggcgtgct gacgatcacc gctcacatga agcccggctt tggccgaccg aagcggatcc
    27841 ggatcgcggg aaagggagcg agccagggga ctggcaccca gcagcaatcg gcaacggctc
    27901 agccgggttc gagccagcag acccagagcg ataagcctga ccaaggagcg caggctggtc
    27961 aacagacccg tccggccaac gggcaatgac gcggatgccg acggcgtcgc ctgccgtgct
    28021 cgaatcgcgg ctgggccggg atgttactcg taagaactgg ggaagaagga gagataaagg
    28081 atcgccagca cgaggacggc gatcccaaac gacaggatga ggacacgcag gttggcacgg
    28141 ttgcggctgc cctggcgagc ttcgacgggc gttcgctgaa ccgcccgctc ctcgtcgtca
    28201 tgatgtggac tgtgatttgc catgcttgtt gaagcacttc cgagtgaaag cgggcgacta
    28261 ccgccgctgg gacacaggcc cacggcctct ggcggatcat cgatgctgat gaccagtcct
    28321 ggtcaggctg cccggcggga tctctgatcg aagaaaagcg cctggcttat cgtcacacgc
    28381 agggtttcag gctggtacgg cttggtgatc aggaatgtgg gttccggctt ctcgccggtc
    28441 agaagcttct ctggatatgc cgtgatgaag atcaccggta cctcgaacga tccgagcaga
    28501 tcattgaccg cgtccagccc ggagctcccg tccgccagct gaatgtcggc cagcactaga
    28561 cccgggcgct gcttggcaat tgctttgcgc gcctcgtccc tcgtacgtgc cacggcgatg
    28621 acacgatggc caagctcctc gagaatctgc gtcagatcca ttacgatcag cggctcgtcc
    28681 tcgatgatca aggcgtccgt tgcgacctga cgggcgatct cctcgcc
//
LOCUS       DASVDU010000270         4262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1047200, whole genome shotgun sequence.
ACCESSION   DASVDU010000270 DASVDU010000000
VERSION     DASVDU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047200"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VF226_18235"
     CDS             <1..832
                     /locus_tag="VF226_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7075981.1"
                     /translation="GWTGKLWAMQQGLQHATERGTSPDYILFVDADIVLAPDIVQQLV
                     NLAERRRTALASLMAKLRCESLPERMLIPAFVFFFQKLYPFSWVNRADCPTAGAAGGC
                     MLVRRAALEDAGGLRAIRSALIDDCALARLLKRQGPIWLGLSRDVVSLRPYPGFNDIR
                     RMVTRTAFAQLGYSHWQLAAAVLAMSVAYLAPPIAAFAGHGATQFVGAAAWALMAFMF
                     LPTLRSYGVSLLWAPALPLIAALYTAFTLESAAQSWRGHGGSWKGRFQSIPIRGHHSP
                     "
     gene            832..1713
                     /gene="hpnC"
                     /locus_tag="VF226_18240"
     CDS             832..1713
                     /gene="hpnC"
                     /locus_tag="VF226_18240"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX7075982.1"
                     /translation="MNATAPKPSKTHRDENFPVASWVVRAEHRPIILAFYRFVRTADD
                     VADNPNLQPAEKLAMLDRLEAALLGRSTETDASAEPLRIILRERELSPAHALELIEAF
                     RLDVRKSRYASWSELMDYCRLSAMPVGRFVLDVHGAPAALWAYSDPLCAALQVINHLQ
                     DCAADYKALDRVYIPLDVLSRHGASVADLAAPRASTGLQSCLRELAARAGELVDASAP
                     LPRGLDWRLGIEVATIQQAATRLVALLSRRDPLCQRVHLRKANFAAAFLSALFGEAAA
                     RLRRRPAAAARETEPAR"
     gene            1710..2543
                     /gene="hpnD"
                     /locus_tag="VF226_18245"
     CDS             1710..2543
                     /gene="hpnD"
                     /locus_tag="VF226_18245"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEX7075983.1"
                     /translation="MTIAVGYQPHLGNSPNNSSFYLALRVLPAAKREAMYSVYGFCRA
                     VDDVADSDTDPERRLTVLARWREEIDHLYGRERHSRLETLRNAIVHFGLERRDFLAII
                     DGMEMDVRGELWAPDWPQLELYCDRVASAVGRLSVRIFGLDDDHGLPLAHHLGRALQL
                     TNILRDLDEDASLGRLYLPREALEAASIRSPYADALDHPCLDDACQLVAQRALHHFEA
                     ADAVMERCPRSLVRTPLIMATVYRSILAQLLARGWQPPRTRISTSRVRAIAAVLRYGL
                     F"
     gene            2543..3829
                     /gene="hpnE"
                     /locus_tag="VF226_18250"
     CDS             2543..3829
                     /gene="hpnE"
                     /locus_tag="VF226_18250"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HEX7075984.1"
                     /translation="MSGTVHIVGAGLAGLSAAVRLAGSGQRVKVYEGAPQAGGRCRSY
                     FDLLLQRTIDNGNHLLLSGNSLALDYLARIGAAHELKGPADAVFDFADIGTGKRWTLR
                     LDPGLFPRWILDPDRRVPGSLLREYIAPIRLIGAGPSARLSDVMTCSGPLYDRLWRPL
                     LLAALNTEPAEASARLAWQVLRRTIGAGGRACLPLIAQHGLSAAFIDPAREFLAKHNA
                     DVAFGKALRAIAVGSGRAQALAFDRARVTLNAEDAVILAVPPRVAAALLPDLDTPNAF
                     SAIVNAHFAITPPEHLPPVLGIVGGLSQWLFAYDDRLSVTISDAGHLLAQDRAELAAA
                     IWHEVARLTGLPEQLPPWQIVKERRATIRATPEQEAKRPAQQTPWANLVLAGDWTATG
                     LPGTIEGAIQSGYQAAAIIAKRQQCRSMHVLEEADG"
     gene            3822..>4262
                     /locus_tag="VF226_18255"
     CDS             3822..>4262
                     /locus_tag="VF226_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX7075985.1"
                     /translation="MDRRAISRGDVSGPPLLVDVSAAVEKAAQALIELQQADGHWCFQ
                     LEADATIPSEYVLLTHYLGEEPKPGLEGKIASYLRRIQESHGGWPLFHGGKLDVSASV
                     KAYFALKMIGDPPDAPHMVRAREAILAAGGAARSNVFTRFLLALY"
BASE COUNT          665 a         1466 c         1351 g          780 t
ORIGIN      
        1 gggctggaca ggcaagctct gggccatgca acagggcctc cagcacgcga cggaacgggg
       61 gacctcgccc gattacatcc tgttcgtcga cgccgacatc gtgctcgccc ccgacatcgt
      121 gcaacagctt gtgaacctcg ccgagcgccg tcgtactgct ctcgcgtcat taatggccaa
      181 gctgcgctgc gagagcctgc ctgagcggat gctcatcccg gccttcgtct tctttttcca
      241 gaaactctat ccattttcct gggtcaaccg cgccgactgt ccgaccgccg gcgccgcggg
      301 cggctgtatg ctggtccgcc gcgctgcgct ggaagacgcg ggcgggctgc gagccatccg
      361 ctcggctctg atcgacgact gcgcgctcgc tcgtctcttg aagcggcaag gcccgatctg
      421 gctcggcctg tcgcgcgacg tggtcagcct gcgcccctat cccggcttca acgatatccg
      481 gcgcatggtc acgcggaccg cgttcgcgca gctcggctat tcgcactggc agctggcggc
      541 cgctgttctg gcaatgtcgg tggcttattt ggcgcctccc attgcggctt tcgctggcca
      601 cggcgcgacg cagtttgtcg gcgcggcagc ctgggcgctg atggccttca tgtttctgcc
      661 gacgctccgc tcttatggcg tgagccttct ctgggcaccc gcgttacccc tcatagcagc
      721 gctctatacg gccttcacct tggaatcggc agcccagagc tggcgagggc acggcggctc
      781 ctggaagggc cgcttccagt caattccgat caggggtcat cactcaccat gatgaatgcg
      841 acggccccta agccaagcaa aacccaccgc gacgagaatt ttcccgtggc gtcatgggtc
      901 gtccgcgccg agcatcgccc gatcatcctt gccttctatc gtttcgtccg gacggccgac
      961 gacgtcgccg acaatcctaa cctgcagccg gccgaaaagc tggccatgct cgaccggctc
     1021 gaagccgcac tcctcggacg gagcacggaa acggacgcga gcgccgagcc gctgcgcatc
     1081 atcttacgcg agcgtgagct gtcccctgct catgccctcg agctgatcga agcctttcgc
     1141 ctcgacgtcc gcaaatcgcg gtatgcgagc tggtccgagc tcatggacta ctgccggctt
     1201 tcagcgatgc ccgtcggccg cttcgtgctc gacgtgcacg gcgcgccggc cgcgctctgg
     1261 gcctactccg atcctctgtg tgcggccttg caggtgatca accacctgca ggattgcgct
     1321 gccgactaca aggcgctcga ccgggtctac atccccctcg atgtcctgag ccggcacggg
     1381 gcctctgttg ccgatctcgc cgccccgcgt gcaagcacag gcttgcagag ctgcttgcgg
     1441 gagcttgccg cgcgcgccgg tgagctcgtt gacgcgagcg cgcccctccc gcgcggcctg
     1501 gactggcggc tcggcattga ggtcgcgacg attcagcagg ccgcgaccag gctcgtcgcg
     1561 ctgttgagcc gccgcgatcc gctctgtcag cgcgtgcacc tgcgcaaggc gaacttcgca
     1621 gccgcatttc tatcggcact gttcggagag gccgcagctc gcctgcgtcg ccgtccggcc
     1681 gccgccgcgc gagaaacgga gccggcccga tgaccatcgc cgtcggctat cagccgcacc
     1741 tcgggaattc gcccaacaac agctcgttct atcttgccct gcgggtgctg cccgccgcga
     1801 aacgggaggc catgtattcc gtctacggct tctgccgggc cgtggacgac gtggcggaca
     1861 gcgatacgga cccggagcgc cgattgacgg tcctcgcgcg ctggcgcgag gaaatcgacc
     1921 acctctacgg ccgcgagcgc cactcccgtc tcgaaaccct gcggaatgcg atcgtgcatt
     1981 tcggtctgga gcgccgggat ttcctcgcga tcatcgacgg gatggaaatg gatgtcagag
     2041 gcgagctgtg ggcacctgac tggccccagc tcgagcttta ttgcgaccgg gttgcgagcg
     2101 ccgtcggccg gctgtcggtg cgcatcttcg gcttggatga tgaccacggg ctgccgctcg
     2161 ctcaccatct cggacgcgcc ctgcagttga ccaacatcct ccgtgatctc gatgaggacg
     2221 cgagccttgg gcggctctac ctgccgcggg aggcgctcga ggcagcaagc atccgcagtc
     2281 cctacgcgga tgcgttggac catccgtgcc tggatgatgc ttgccaactg gttgcccaac
     2341 gtgcccttca ccacttcgag gcagcggacg ccgtgatgga gcgctgcccg cgctcccttg
     2401 tccgcacacc gctgatcatg gcgacggtct accgctcgat cctggctcag ctcttggcgc
     2461 ggggctggca gccgccgcgg acacgaatct cgaccagccg ggtgcgagcg atcgccgcgg
     2521 tcctgcgcta cgggctgttt tgatgtcggg cacggttcac atcgtcggcg cagggttggc
     2581 cgggttgagc gccgccgtcc ggctcgccgg cagtggccag cgcgtcaaag tctacgaggg
     2641 tgcgccacaa gcgggcgggc gctgccggtc ctatttcgac ctcctccttc aacgcaccat
     2701 cgataacggc aaccatcttc tgctgtcggg caatagcttg gcgctcgact atctcgcccg
     2761 tatcggtgcc gcgcatgagc tgaagggccc ggcagatgct gttttcgatt tcgccgacat
     2821 cggaacgggg aagcgctgga cgctgcggct tgatccaggc ctcttcccgc gctggattct
     2881 ggacccggac cggcgagtcc ccggcagcct gctccgcgaa tatatcgcgc cgatcaggct
     2941 catcggtgcg ggcccgtcgg cccggctatc ggacgtgatg acctgctcgg gtccgctcta
     3001 tgatcggctc tggcggccgc tgctgctcgc tgcactcaat accgagccgg cagaggcttc
     3061 agcccggctc gcttggcaag tgctccgccg gaccatcggt gcgggcgggc gcgcctgcct
     3121 gcccctcatc gcgcagcatg gattgtcggc ggcattcatc gatcccgcac gcgagttcct
     3181 ggcaaagcac aacgccgacg tggctttcgg aaaggcgctt cgcgccattg cagtcgggtc
     3241 aggacgggcg caagccctcg cgttcgatcg ggcgcgcgtc actctgaatg cggaggacgc
     3301 cgtcattctg gcggtgccgc cgcgcgtggc cgcggccctt ctgcccgacc tcgacacgcc
     3361 gaatgccttc agcgccatcg tgaatgcgca tttcgcgatc actccgccgg agcaccttcc
     3421 acccgttctc ggaatcgtcg gtggcttgag ccaatggctc tttgcctacg acgaccggct
     3481 ttcggtgacc atcagtgatg ccggccacct cctggcacag gatcgcgccg agctggccgc
     3541 cgcaatctgg cacgaggtgg cgcgcttgac cggcctgcca gagcaactgc cgccgtggca
     3601 gatcgtgaag gagcggcggg caacgatcag ggctacgccg gagcaggaag caaaacgccc
     3661 ggcccagcaa acgccctggg ccaatctcgt tctcgccggc gactggacgg cgacggggct
     3721 gcccgggacc atcgagggcg caattcagtc aggctaccag gcagccgcaa tcattgcgaa
     3781 gcgtcagcaa tgccgctcca tgcatgtgct ggaggaggcc gatggataga cgagcgatca
     3841 gccgaggtga tgtttcgggg ccgccactgc tcgtcgatgt gagcgcagca gtggaaaagg
     3901 cggcgcaggc gcttatcgag ctgcagcagg cagacggcca ttggtgcttc cagctcgaag
     3961 cggacgcgac catcccatcc gaatacgtgc ttcttacgca ctacctcggc gaggagccga
     4021 agcccgggct cgagggaaaa atcgcctcgt atcttcgtcg catccaggag agccacggcg
     4081 gctggccgct gtttcatggc ggcaagctcg acgtcagcgc cagcgtgaag gcctacttcg
     4141 cgctcaagat gatcggggat ccgccagatg ccccccacat ggtccgcgcc cgtgaagcca
     4201 ttctcgccgc gggtggagcc gcaagaagca acgtctttac gcgctttctg ctcgcactct
     4261 ac
//
LOCUS       DASVDU010000271         7944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1211821, whole genome shotgun sequence.
ACCESSION   DASVDU010000271 DASVDU010000000
VERSION     DASVDU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211821"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF226_18260"
     CDS             <1..334
                     /locus_tag="VF226_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX7075986.1"
                     /translation="LLLHGHCHQKSFNAMPAVTKVLSQVEGLTVDVVQSSCCGMAGAF
                     GYAAETYDISMAMGELSLLPAVRKAEADCAIAADGFSCRHQIKDGTGRQARHVALVLR
                     DVLAASEG"
     gene            complement(540..1040)
                     /locus_tag="VF226_18265"
     CDS             complement(540..1040)
                     /locus_tag="VF226_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075987.1"
                     /translation="MGLEGYLSAAEAFDGASAAPAAAMPMPIVKARAQFFICRLLQPN
                     AGPIRGAAPRRPAESGLPQGVPVSLSARTPQTAMDAEPFRMPKASTLCAISGTWRDDV
                     NASMRNLMIEKICCALDARIRAKFGMGHCQLSFGAYFAAREYELFAGSTAYSASACAK
                     TLTTQM"
     gene            931..1896
                     /locus_tag="VF226_18270"
     CDS             931..1896
                     /locus_tag="VF226_18270"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7075988.1"
                     /translation="MKNWARALTIGIGIAAAGAALAPSNASAADKYPSRPIEFIVPWG
                     PGGGADQLARLTGKLLEPEIGTSVPVVNVPGATGGTGMAKLLAAPADGYSMAIYIADS
                     HALLAGPEPRWQMDDITPVAVMIQGPSFIFVADNSRFKSWADFEKEAKEKPGTLKVAT
                     LGFGSVDDYTLRYLEGKGIKVVQVPFSKPSERYVSILGGHADALYEQAGDVGQFLKSK
                     QMRPLIIFGEKRHPDFPDTPSSYELGYEVALPQFRSIVVRSGTPEEHVKVLSEGLAKV
                     AATEEFKKFLSDQFADPNSFIDASHAGEFIKKQLEDMKKLAGAKS"
     gene            2017..2574
                     /locus_tag="VF226_18275"
     CDS             2017..2574
                     /locus_tag="VF226_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7075989.1"
                     /translation="MAQRVKAALPYVVGLAIAAVLYHMAGTISYPARPGQLGPDFWPR
                     LAIAIIAIVCIYEISRLIIVGSAHNSVQGIAEHLEAEELDEEPGRANASSRIYMLLGG
                     LALTFAYAVLVPILGFLLASYIFLVVFMYLGGNRNHLAIWASSTIGILVFAFIFLKVV
                     YVSLPRGVPPFEQVTQFIMDLFQIS"
     gene            2759..4279
                     /locus_tag="VF226_18280"
     CDS             2759..4279
                     /locus_tag="VF226_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7075990.1"
                     /translation="MLEIFTQLGAGFINCMSPANLLMLVVGIVVGLLVGVLPGLTLVM
                     GVILVLPFTYQMDITAAIVLLTAMYVSGTYGGAFTAILFRIPGEPLDVPMLWDGYPMA
                     QKGRAADALGWTLMAALGGGFVSAIVMVLLTQPVATFALKFSTPEYFAIVLFGLASVI
                     ALGRGALPNAFLSLGIGLLIASVGTDVIYGSYRFTFGSPILADGIEFLVVMVGAYGVG
                     EVFSRLEQGFATKPVEQISETSTRLPSFRELFALRWMFLRSSLLGNIIGVVPGAGATI
                     ASFVSYGVEAQYGKRRKEMGTGIPEGIVAPQAASTASVGGALVPLLALGIPGSGATAV
                     MLGAFMLQGVQPGPQILLTSSGMVYTIFASVFLGIALMCVLGFYFIRMLVKVLDFPEA
                     IVSAFVMLLCFIGAMSIRNNLTDLWLMLAFGVIGYLLERFRFPIAPMVLGVILGPIAE
                     EAFMNTMISFGNDWTVFFTRPISGSIMAAVIITLLLPFLGRLLRRSQARTASSEAS"
     gene            complement(4612..5496)
                     /locus_tag="VF226_18285"
     CDS             complement(4612..5496)
                     /locus_tag="VF226_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075991.1"
                     /translation="MASLGNAPKDQRTTGTGLKQFVLAMTMIGLLGLPAVRAPVDAQT
                     KPIINVASVVLAEPAIETPFPIQIGPAEAIPKQTFLRIRGLPSTATLSEGHAIAAGAW
                     AIPLSGLPRLKIATPAGASGKADITISLVAIDGRILSEVKATLLIAPASAITPAASQA
                     AGGQAGANVAALSPAGDALGSVPARLPLQPPVPPPPDIPAEDREQALIYMDRGDESLA
                     NGNVTVARLFYEKAAELNWGPAALALAATYDPEELANWNVLGGVEPDPEEARKWYERA
                     RELGTAEAVERLQRLGAR"
     gene            complement(5567..6583)
                     /locus_tag="VF226_18290"
     CDS             complement(5567..6583)
                     /locus_tag="VF226_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7075992.1"
                     /translation="MARTNAWTVSIASGLLEGTFSRFAAELAKALDDGDNLRVLPIIT
                     YGAAENLSDLLYLKGIDMAITHADVFDEFKRNRRAANLEQRINYISQMYVGELHVFAR
                     HDIKSLKDLEGKKVGFHTRGAGPTVTGPILFSRLGIKVEPVFINNAIAIEKMKTGEIA
                     AILHTVGKPNILFANLTSDVGHFLPVEYGEKFADYYVPSTLGYEDYPNLFEPGEKIDT
                     IGIPVVLAVYNWPEGHDRHRRVERFIERYFTHFDTLRKPPYHPKWKEINLAANVPGWN
                     RYFVAQRLLDQMQPATAAAGIDPDLARSQAARVAPNDRAEQERLFQEFLAWSRQRARR
                     QARQ"
     gene            complement(6962..7255)
                     /locus_tag="VF226_18295"
     CDS             complement(6962..7255)
                     /locus_tag="VF226_18295"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX7075993.1"
                     /translation="MAADQSASQPTQEERIQRAKERVSEIKGFYIHFAIFVVVLSVLF
                     IVDATTSGGWWVQWVFLGWGIGVLAHAAAIFGSTPNFVRRWEERKVRQYLDES"
     gene            complement(7352..>7944)
                     /locus_tag="VF226_18300"
     CDS             complement(7352..>7944)
                     /locus_tag="VF226_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7075994.1"
                     /translation="GGGGFGGGFGGGGGGLGGGGGGGRGIGGGGFGGGGPSFGGGGPR
                     FGGGGPSFGGGRGGGGFPGRPGLRGGFPGGGGGPSMGRSPGFQSGPIGRGPGAGRSFE
                     RGPGRGFGGAPIQREFRGRAERGPSPRVGRREFRRGDRRFARRGHWRPGYFWGGVWIG
                     PGLYYANCEWLRRRAYLTGSRYWWRRYNECVAINYS"
BASE COUNT         1432 a         2452 c         2443 g         1617 t
ORIGIN      
        1 cctgctgctc cacggccact gccaccagaa atcgttcaac gcgatgccgg ccgtcaccaa
       61 ggtactttcc caggtggaag ggcttacggt cgatgtggtc caatcgagct gctgcggcat
      121 ggctggtgcg ttcggctatg cggcggagac gtacgatatc tcaatggcga tgggtgagct
      181 gtcattgctg ccggcggtgc gcaaggcgga ggccgattgc gccatcgctg ccgacggctt
      241 ctcctgccgg catcagatca aggacggcac tggccgccag gcacgccacg ttgcgctggt
      301 gctccgcgat gtgctcgccg catcagaagg ctgagagagc tggcgcccag gttcaaaagg
      361 gggatgactc agaggacgcc aaagagatac aagagtaaaa tcaccgagat cggcacgcca
      421 aagagccata ggacaacgcc tttcatgcag cctctcccgg ttgtgagtcc tggatgctga
      481 atcctcgccc agcattgcca agacgaactc cgtggcaggc aagtggttcc ccgcttcatt
      541 tacatttgcg ttgtgagcgt cttcgcgcat gcagaggctg agtacgccgt gctgcccgca
      601 aacagctcgt attctcgggc cgcaaagtag gcgccgaaac tcaattggca gtgccccatt
      661 ccaaattttg ctcggatgcg cgcgtcgaga gcacaacaga ttttctcgat catcaaattg
      721 cgcatcgaag cgttgacatc gtcgcgccac gtgccactga tagcgcacag cgtgcttgct
      781 ttcggcatgc ggaacggctc ggcgtccatg gcggtttgcg gcgtacgagc agacaaggag
      841 acgggcacgc cctgtgggag gcccgattca gccggacggc ggggagctgc cccgcgaatt
      901 gggccggcat ttggctggag gagacgacaa atgaaaaact gggcacgagc cttgacgatc
      961 ggcattggca tcgccgccgc tggggcggcg ctcgcaccat caaatgcttc cgccgccgac
     1021 aaatatcctt cgagacccat cgagttcatc gtgccgtggg ggccgggcgg tggcgccgat
     1081 cagctggcgc ggctgacagg caagctgctg gagccggaaa tcggcacctc ggtgcccgtc
     1141 gtcaatgttc cgggcgcgac aggcggcacc gggatggcga agctgctcgc ggcgcccgcc
     1201 gatggttatt cgatggccat ctacatcgct gacagccatg cattgctggc gggccccgag
     1261 ccgcgctggc agatggatga catcacgccg gttgccgtga tgatccaggg gccgtcattc
     1321 attttcgtgg ctgataatag tcgcttcaag agctgggctg atttcgagaa agaagccaaa
     1381 gagaaaccgg gaacgctgaa agttgcaacg ctcggcttcg gcagtgtcga tgactacacg
     1441 ctgcgttatc tcgagggaaa aggcatcaaa gtggtccagg tgcctttctc caagcccagc
     1501 gagcgttacg tttccattct cggcgggcat gccgatgctc tctacgagca ggcaggtgac
     1561 gtcgggcagt tcctcaagag caagcagatg cggccgctga taatattcgg cgagaaaagg
     1621 catccggact tccccgatac gccgagttca tacgagttgg gttacgaggt ggccctgccg
     1681 cagttccgct cgatcgtcgt tcgctcgggc acgccggagg aacacgtcaa agttctctcc
     1741 gaagggctgg caaaggttgc ggcgacggaa gaattcaaaa agtttttatc ggaccagttt
     1801 gccgacccga acagcttcat cgatgcgtcc catgcgggtg agttcatcaa gaagcagctt
     1861 gaggacatga agaagctcgc gggcgccaaa tcctgatcgg gtccgatgtg gccgctgacc
     1921 gtgctcgctg ctcctgggcg gcgggcacag tcatgaattg aggtcgcgtg aaatgcgcgc
     1981 gaccggcgga cgattcttca aacgggattt cttggaatgg cgcagcgagt gaaggccgcg
     2041 ctcccctatg ttgtggggct cgccattgcg gcagtgcttt atcacatggc cggcacgatc
     2101 agctatccgg ccagaccggg ccagctcggt cccgatttct ggccgcggct tgccatcgcg
     2161 atcatcgcga tcgtctgcat atatgaaatt tcccggctga tcatcgtcgg gtcggcccac
     2221 aacagcgtgc agggaatcgc agagcatctg gaagccgagg agctcgatga ggaaccagga
     2281 cgtgcgaacg cgagctcgcg catttacatg cttcttggtg ggttggcgct gacgtttgct
     2341 tatgccgtcc tggttccgat cctcgggttt ctgctcgctt cctacatctt cctggtcgtg
     2401 ttcatgtacc tcggcggcaa tcgcaatcat ctcgccattt gggcctcgag cacgatcgga
     2461 attctggtct tcgccttcat tttcctgaag gttgtctacg tttccctgcc caggggcgtg
     2521 ccaccgttcg aacaggtcac gcaattcatc atggacctgt ttcaaatcag ctagtggccc
     2581 gatcgagaaa gctcgccgaa gcgtgatgag cttgaagcca ttttcggaca ctgaaaggcg
     2641 aaactggaat tttgcagggt cgctggttgg ttgggatcga caaacgggct gaaagagatg
     2701 acacgcgcac acgcaatttt cttcgttggc aaatcaaacg gcgcctgagg atacgcgaat
     2761 gctcgaaatt ttcactcagc tcggtgccgg tttcatcaac tgcatgagcc ccgccaacct
     2821 gctgatgctg gtggtcggca ttgtcgtcgg cctgctggtg ggggtgctgc cggggctgac
     2881 gctcgtgatg ggcgttattc ttgtgctgcc gttcacgtac caaatggaca tcacggcagc
     2941 catcgtgctg ctgacggcca tgtacgtgtc cggcacctac ggcggcgcct tcacggccat
     3001 tcttttccgc attcccggtg agccgctcga tgttcccatg ctgtgggacg gctatcccat
     3061 ggcgcaaaag ggccgcgcgg ccgatgccct cggctggaca ctgatggcgg cgcttggtgg
     3121 cggcttcgtc tcggcgatcg tgatggtgct cctgacgcag ccggtagcga cgtttgctct
     3181 caagttctcg acgccagagt atttcgccat cgtcctgttc gggctggcga gcgtcatcgc
     3241 gctcggccgc ggggcgctgc cgaatgcgtt cctatcgctc ggcatcgggc tgctgatcgc
     3301 gagcgtcgga acggacgtca tttacggcag ctaccgcttc accttcggct cgccgatcct
     3361 ggcggacggc atcgagttcc tcgtcgtcat ggtcggcgcc tacggcgtcg gtgaggtctt
     3421 cagccggctg gagcaggggt ttgccaccaa gcccgtcgag cagatttccg aaacctcgac
     3481 aaggctccct tcgttccgcg agctcttcgc gttgcgctgg atgttcctgc gctcaagtct
     3541 tctcgggaac atcatcggcg tggtgcccgg agctggagcg accatcgcct ccttcgtttc
     3601 ctacggggtc gaggcacaat acgggaagcg gcggaaggaa atgggcactg gcatccccga
     3661 aggcatcgtt gcgccgcagg ccgcctcgac ggcgtcggtg ggcggcgcgc tcgtgccgct
     3721 tctggcgctc ggcatcccgg gcagcggcgc gacggccgtc atgctcggcg cgttcatgct
     3781 gcagggggtg cagccggggc cgcagatcct gcttacgtct tcggggatgg tctacacgat
     3841 tttcgcctcg gtctttctcg gcatcgcgct gatgtgcgtg ctcgggttct atttcatccg
     3901 catgctcgtg aaggttctcg attttccgga ggcgatcgtt tcggccttcg tcatgctgct
     3961 gtgcttcatc ggcgccatgt cgatccgcaa caacctgacg gatctctggc tgatgctcgc
     4021 gttcggagtg atcggctacc tgctcgagcg atttcgcttc ccgattgcac cgatggtgct
     4081 cggcgtgatc cttgggccga ttgccgagga ggcgttcatg aacacgatga tcagcttcgg
     4141 caacgactgg acggtcttct tcacccggcc catttcgggc agcatcatgg ccgccgtgat
     4201 catcacactt ctgcttccct tcctcggccg gctcctgcgg cggtcgcagg cgcgcacggc
     4261 gtccagcgag gcgagttgag tgaagcttcg aaatttctgg agatttttcg atttaggctg
     4321 aagcacaccg ccgcggccga aatcttcctt cccggagggg aggcggcgtt ggccgtcgcg
     4381 ccagccgtgc aagccttgac ccgggctcgg aagaagagcg gggacggcag tcttgcctta
     4441 tctcacgctt atagcttttt ccgaatttgg gtgagcacgg cagcagcagg gccgggtgag
     4501 gggttgtgac agctgccact ggcagaaccc ctctccccac cgcgagaggg agagaggacg
     4561 caagcttggc ctagtgcttc catttacatc ggtaatgatc aggtccgggg ttcagcgcgc
     4621 gcccaagcgc tgcaatcgct cgacggcttc ggcagtgccg agctcacggg cgcgctcgta
     4681 ccacttgcgc gcttcttcag gatcgggctc gacgccgccc aggacgttcc agttcgcaag
     4741 ctcttccgga tcataggtgg ccgcgagcgc caaagcggcg gggccccaat tgagctcggc
     4801 agccttctcg tagaagaggc gtgcgactgt cacattaccg ttggccaggc tttcgtcgcc
     4861 ccgatccatg taaatgagag cctgctcccg gtcctcggcg gggatgtcag gcggtggcgg
     4921 aacgggtggc tgcagaggaa gccgcgcggg taccgatccg agggcatctc ccgccgggct
     4981 gagcgcggca acattggccc ccgcttgacc gcccgccgcc tggctcgccg ccggcgtgat
     5041 cgcggaagcc ggcgcaatca gcagggtcgc tttcacctcg ctcaggatac ggccgtcgat
     5101 tgcgacgaga gaaatcgtga tgtcagcctt gccagatgca ccagctggcg ttgcgatttt
     5161 cagccgcggc aggccactca gcggaatggc ccaggccccg gcggctatgg cgtgaccctc
     5221 ggatagcgtg gccgtgctcg gcaggccgcg gattctcagg aaagtctgct tcggaatggc
     5281 ctcggccggg ccgatctgaa ttgggaaagg cgtctcgatg gcgggctccg caaggaccac
     5341 cgaggcgacg ttgatgatcg gcttcgtctg cgcatcgacg ggagctctga ccgccggaag
     5401 acccaacagt ccgatcattg tcattgccag aacgaactgc ttcagcccag tgccagtcgt
     5461 ccgttggtcc ttcggcgcat tccccagtga cgccatcggt tccctcccgc cgagttggcc
     5521 gttttacctc ctcaaccgcg cttattcttg ttctgtagcg gcggccttat tgccgggctt
     5581 gtcgcctggc cctttgcctg ctccacgcga gaaactcctg gaatagacgc tcctgctcgg
     5641 cgcgatcatt tggtgcgacg cgcgcggctt gggaccgggc aaggtccgga tcgatgcctg
     5701 ctgcggcagt cgccggctgc atctgatcga gcagcctttg cgcaacgaaa tagcggttcc
     5761 agcccggcac gttggctgcc agattgatct ctttccattt cggatggtac ggcggtttcc
     5821 tcagcgtgtc gaaatgcgtg aaatagcgct cgatgaagcg ttcgacgcgg cgatgccggt
     5881 cgtgcccctc gggccagttg tagaccgcga ggacgacggg aatgccgatc gtatcgatct
     5941 tctcacccgg ctcgaacaga ttggggtagt cttcgtatcc caaggtcgag ggcacgtaat
     6001 aatcggcgaa tttttctccg tactcgaccg gcaggaagtg cccgacatcg gacgtcaggt
     6061 tcgcgaacag gatgttcggc ttgcccacgg tatgcaaaat cgcggcaatc tcgcctgtct
     6121 tcatcttctc gattgcgatt gcgttgttga tgaagacagg ctcgaccttg attccaagcc
     6181 gcgagaacag aatggggccc gtcaccgtcg ggcctgcgcc cctcgtatgg aagccgacct
     6241 tttttccctc gagatccttg agtgacttta tgtcgtggcg cgcgaacaca tgcagctcgc
     6301 cgacgtacat ctgcgagatg taattgatgc gctgctcgag attggcggcc cttctgttcc
     6361 gcttgaactc gtcgaagaca tcggcatgcg tgatcgccat gtcgatgccc ttgaggtaga
     6421 gcagatcgct caggttctcc gcggcaccgt atgtgattat cgggagcaca cggagattgt
     6481 cgccgtcgtc caatgccttt gcgagctcgg cggcaaaacg ggagaaggtc ccttcgagca
     6541 ggccgcttgc aatggagacc gtccaggcat tggtgcgggc cactatcgca gcctcacctg
     6601 atgccgcagc tgtcggcacc gtgcgcgctt gcgcaacatc aaacggttgc ggcgtttgcg
     6661 cccaggcatt tccggcaacg aggggcgcta aactgatcgc tcccgcaacc cacactagca
     6721 agccacgcac ggacacgtct cccgtctgaa tgtttaggtg ctgccttcag gcataagaga
     6781 ggtctgaatt gggccatatt atagcagacg aggcactggc gtgaaatcaa agcttgccga
     6841 tcgtccctca acggagatgg cggagtagga tcaaaggctt atgtccgtta tcggacattg
     6901 ccattattgc gtccgaatgt agttggtcgg cttgtgtcgc tacttggaat tgaactaagc
     6961 ctcagctctc atccaagtac tggcggacct tgcgctcttc ccaccttctg acgaaattcg
     7021 gtgtgctgcc gaagatggca gccgcatggg cgaggacgcc gataccccag cccaggaaca
     7081 cccattgcac ccaccagcca ccgctcgtcg tggcatcgac gataaagagc acagaaagca
     7141 ccacgacgaa gatcgcgaaa tgaatataga agcccttgat ttcggaaacg cgctccttcg
     7201 cacgctgaat ccgctcttcc tgcgtcggct ggcttgcgga ttggtcggca gccatcgtcc
     7261 tgtacctccg cgcctggtca tgcctgcagc cggacgatgt cgcgccggct gcatcgtgcc
     7321 tgttcgacct gagcctagta cgaggtcggc gtcaggaata gttgatggcg acgcactcat
     7381 tatagcgacg ccaccagtag cgagagccgg tgaggtacgc ccgccgcctc agccattcgc
     7441 aattggcgta gtaaaggcca gggccgatcc agactccgcc ccagaaatag ccgggacgcc
     7501 agtgtccgcg ccgggcaaat cgccggtcgc ctctccggaa ctccctgcga ccaacgcgag
     7561 gtgacgggcc acgttccgct ctgccgcgaa actcgcgctg gatcggcgca ccgccgaagc
     7621 cccggccagg accgcgctcg aagctccggc ccgctcctgg tccccggccg atcggcccgc
     7681 tctggaaacc ggggctgcgg cccatcgagg gaccaccacc ccctcctggg aagccgcccc
     7741 gcaggccagg gcggccaggg aacccgccgc cgccacgacc tccaccgaag ctcggacccc
     7801 ctccgccaaa gcgggggcct cccccgccga agctcggacc tcccccaccg aagcctcctc
     7861 cgccgatgcc gcgtccgcct ccgccgccac cgccgagccc accaccaccc cctccaaatc
     7921 cgcctccaaa tccacccccg ccac
//
LOCUS       DASVDU010000272         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_453087, whole genome shotgun sequence.
ACCESSION   DASVDU010000272 DASVDU010000000
VERSION     DASVDU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453087"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..430
                     /locus_tag="VF226_18305"
     CDS             86..430
                     /locus_tag="VF226_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX7075995.1"
                     /translation="MELVISITIGILAGSGLWLILRPRTFQVIIGLSLLSYAVNLFIF
                     GMGRLRVGKAPVLDATVPPYLAGYADPLPQALVLTAIVISFAMTALFLVVLLAARGLS
                     GSDHVDRVERGS"
     gene            427..2064
                     /locus_tag="VF226_18310"
     CDS             427..2064
                     /locus_tag="VF226_18310"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX7075996.1"
                     /translation="MIPADHLVIAPIIIPLVAGGLMLLFEGRRREIVATINIVATLAL
                     LAVSIVLLVGSMGEDPARAVKVYLLGNWPAEFGIVLVADRLAALMLLLTAILGCAALL
                     FALASWHRAGSYFHPLFQFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLALHGSGRA
                     RVRSSLHYIVINLAASSFFLIGVSVIYGTAGTLNMAAVAERVAALDPSERGLFEIGAS
                     ILGIAFLVKAAAWPLGFWLPPLYAAASPPVAAVFAIMTKVGLYVILRVGSITAEAGGG
                     TSLFFAEGGLFIIGIATISFAAIGMLASQDMGRLAGYSVLVSSGTVLAAIGVGGAGMT
                     SAALFYLVSSTLTVAAFFLLVELVERGRTPADDVLAVTLEVYGADDEEDAEVESEVGI
                     AVPAIMGVLGVAFLACGLLLAGLPPLSGFVAKFAILSNLLDQHWLNVGGSVSIQGWLL
                     LAVILISGLTTVIAVTRAGIRTFWTFTDRELPHVRATELSAVGLLLVLCVGLTIQAGP
                     VMRFMQTTANGLELGRPYIGAVTGKAQTTPEQPEQTQ"
     gene            2061..>2164
                     /locus_tag="VF226_18315"
     CDS             2061..>2164
                     /locus_tag="VF226_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067024.1"
                     /note="subunit E of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX7075997.1"
                     /translation="MKAWLPYPLISVLLLAIWLLLNQSLALAHILLGGA"
BASE COUNT          303 a          667 c          685 g          509 t
ORIGIN      
        1 tggtcctcat cgctctcgcg caccaggccc tccgcatccc acgtcccgtc agcgaggcga
       61 cgcaacccgt cggagaaaca acctgatgga actcgtcatt tccataacca tcggcatcct
      121 ggccggatcg ggtttgtggc tgatcctgcg gccacggacg ttccaggtga tcatcgggct
      181 ctcgctgctc tcttatgcag tgaatctctt catcttcggc atgggacggc tgcgtgtggg
      241 caaggcgccg gtgctcgatg cgacggttcc gccttacctc gcgggctatg cggatccgtt
      301 gccgcaggcg ctggtcctga cggccatcgt catcagcttc gcgatgacgg ctctgtttct
      361 cgtcgtcttg cttgccgctc gggggctttc tggtagcgat cacgtcgaca gagtggagcg
      421 gggctcgtga ttccggcgga tcacctcgtc atagccccga tcattattcc gctcgtggca
      481 ggcggattga tgctgctgtt cgaggggcgt cgtcgtgaga tcgttgcaac aatcaatatt
      541 gttgcgacct tggcgctgct cgcagtctct atcgtcctgc tcgtcggaag catgggggag
      601 gacccggccc gcgccgtcaa agtctatctt ctcggcaact ggccggccga attcggcatc
      661 gttctggtgg ccgacaggct cgccgcgctc atgctgctgc tcacggcaat actcggatgt
      721 gcggcgttgc tgttcgcctt ggcctcctgg caccgggctg gcagctactt tcacccgttg
      781 ttccagttcc ttctcatggg gctgaatggc gccttcctga cgggcgatct cttcaatctc
      841 ttcgtgttct tcgaggtgct gctggcggca tcctatggtc tggcactgca cggctcgggg
      901 cgcgcacgtg tgcgctcgag cctgcactac atcgtgatca acctggctgc ttcatcgttc
      961 ttcctcatag gcgtcagcgt gatctatggc accgcgggga cgctcaacat ggccgcggtc
     1021 gctgagcgtg ttgccgcgct cgatccctcc gagcgggggc tcttcgagat cggggcttcg
     1081 atcctcggca tcgccttcct ggtgaaggct gcggcatggc ccctcggctt ttggttgccg
     1141 cccttgtatg cggcagcaag ccctccggtg gccgcggtgt ttgcgatcat gaccaaggtc
     1201 ggcctctacg tgatcctgag ggtggggtcg atcaccgccg aggccggtgg cggcacgtcg
     1261 ctgttctttg cggaaggcgg gctgttcatc atcggtatcg cgacgatatc ctttgccgcc
     1321 atcgggatgc ttgcatcgca ggacatgggg cgtcttgccg gatattccgt tctggtctcg
     1381 tccggaacgg tattggcggc catcggcgtc ggcggtgcgg gcatgaccag cgcggcgctg
     1441 ttctatctcg tgagctcgac gctgacggtc gctgccttct tcctgctcgt cgagctcgtg
     1501 gaacgtggcc gcacgcctgc tgacgacgtc ttggccgtga ccttggaagt ctacggtgcg
     1561 gacgacgaag aagatgcgga agtggagagc gaggtcggca tcgcggttcc cgccatcatg
     1621 ggtgtgctgg gtgtcgcgtt tctcgcctgt gggttgctgc ttgccggcct tccgccgctt
     1681 tccgggttcg tcgcgaagtt cgccatcttg agcaacctgc tcgaccagca ctggctgaat
     1741 gttggcggca gcgtttcaat tcagggatgg ctgcttcttg cggtgatcct gatctccggt
     1801 cttacgaccg ttatcgccgt gacacgtgcc gggatacgga ccttctggac gttcaccgat
     1861 cgcgagctgc cgcatgtccg cgccacggag cttagcgccg tcgggttgct gcttgtcctc
     1921 tgcgttggcc tcacgatcca ggccggaccc gtaatgcggt tcatgcagac gacagccaat
     1981 gggctggagc tgggacggcc ttatatcgga gcggtgacgg ggaaagccca aaccacgcct
     2041 gagcagccgg agcagacaca atgaaagcct ggctgccgta tccactcatt tcagtcttgc
     2101 tgctcgcgat ctggctgctg ctcaaccaga gcctcgctct cgcgcacatt ctgctgggcg
     2161 gagc
//
LOCUS       DASVDU010000273         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_453089, whole genome shotgun sequence.
ACCESSION   DASVDU010000273 DASVDU010000000
VERSION     DASVDU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453089"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..868
                     /locus_tag="VF226_18320"
     CDS             104..868
                     /locus_tag="VF226_18320"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX7075998.1"
                     /translation="MVAQIQIRRIPFDAPWDWLAAGWRDLCSVPQVSLAYGAAFAVLA
                     LAIAAGLLQFGMQSLILALAGGFLLIGPLVAVGLYETSWRLETGKPISLREVAFAGLK
                     APGQLGFFGVILTFAFAVWVQLAFLMFMLFMGSRVLPPANEFVPTLLFTPHGLGLLVA
                     GTVVGGVLATIIFSISAISVPLLLTRPVDAVTAMAASVEAVVKNPKPMALWAALIASF
                     MALGTASLFVGLAIAFPLIGHATWHAFRSLVVQNEP"
     gene            complement(1000..1368)
                     /gene="mnhG"
                     /locus_tag="VF226_18325"
     CDS             complement(1000..1368)
                     /gene="mnhG"
                     /locus_tag="VF226_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HEX7075999.1"
                     /translation="MTIEELPLWAALLISVLLILGSGLALIGSIGLLHFKTFYERVHA
                     PTLGSTLGLFSILTGSMLYFSMTEMRFVLHEFLIGAFLTVTTPVTFLLLVQATLYRDR
                     FEGNDPLADLSRNIRQAIER"
     gene            complement(1365..1646)
                     /locus_tag="VF226_18330"
     CDS             complement(1365..1646)
                     /locus_tag="VF226_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEX7076000.1"
                     /translation="MSGLILEISLLVAKIFLTLAMAVTAYRMLRGPRAQDRVLALDTM
                     YVNAMLLLLVFGIGSGATIYFEMALVIGLLGFVATVAMAKFLMRGEVIE"
     gene            complement(1643..2128)
                     /locus_tag="VF226_18335"
     CDS             complement(1643..2128)
                     /locus_tag="VF226_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX7076001.1"
                     /translation="MKAWLPYPLISVLLLATWLLLNQSLALAHILLGGALSVIGPWFL
                     VRLDTPRLIVRRPMAVLQLCCELVVDIVRSNYRVASLILSDRPGRTPGFVWIPLRMHS
                     QYGLAALACIITATPGTSWVAYDPETNILVIHVLDLVDDDDWGKIIKMRYERLLMEIF
                     E"
     gene            complement(2125..3762)
                     /locus_tag="VF226_18340"
     CDS             complement(2125..3762)
                     /locus_tag="VF226_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX7076002.1"
                     /translation="MISADHLVIAPIIIPLVAGGLMLLFEGRRREVVATIDIVATLAL
                     LAVSIVLLVQSMGEDQARAVRVYLLGNWPAEFGIVLVADRLAALMLLLTAILGCAALL
                     FALASWHRAGSYFHPLFQFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLALHGSGRA
                     RVRSSLHYIVINLAASSFFLIGVSVIYGTAGTLNMAALAERVAALDPSERGLFEIGAS
                     ILGIAFLVKAAAWPLGFWLPPLYAAASAPVAAVFAIMTKVGLYIILRLGSITAEAGGG
                     ASLFFAEGGLFTIGIATVSFAAIGMLASQDMGRLAGYSVLVSSGTVLAAIGVGGAGMT
                     SAALFYLVSSTLTVAAFFLLVELVERGRTPADDVLAVTLEVYGADDEEDAEVESEVGI
                     AVPAIMGVLGVAFLACALLLAGLPPLSGFVAKFAILSNLLDQHWLNVGGSVSIQGWLL
                     LAVILVSGLTTVIAVTRAGIRTFWTFTDRELPHVRATELGAVGLLLVLCVGLTIQAGP
                     VMRFMQTTANGLELGRPYISAVTGKAQTAPEQPERTQ"
     gene            complement(3759..4103)
                     /locus_tag="VF226_18345"
     CDS             complement(3759..4103)
                     /locus_tag="VF226_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX7076003.1"
                     /translation="MELVISITIGILAGSGLWLILRPRTFQVIIGLSLLSYAVNLFIF
                     GMGRLRVGKAPVLDATVPPYLAGYADPVPQALVLTAIVISFAMTALFLVVLLAARGLS
                     GSDHVDRVERGS"
BASE COUNT          897 a         1314 c         1297 g          680 t
ORIGIN      
        1 cctccgggct gtcagatgac cgtggctcgt ggccagtgca tgggggcgat tacaacagat
       61 gttcagcaca cgcgatgttg ccggaggctg caacagcagg ccgatggtag cgcagatcca
      121 gatccgacgg attccgttcg atgcgccatg ggattggctg gcggccggtt ggcgcgacct
      181 gtgtagcgtg ccgcaggtga gcctcgccta tggcgcggct ttcgctgtcc ttgccctcgc
      241 gatcgcggct ggattgctgc agttcggtat gcaatcgctg attctcgcgc tcgctggcgg
      301 gtttctgctg atcggtccct tggtggcagt cggattgtac gaaacgagct ggcggctgga
      361 gaccggcaag cccatttcgc tgcgggaggt tgccttcgca ggcttgaagg cgcctggaca
      421 gctcggcttt ttcggcgtga ttctgacatt cgcgttcgcc gtctgggtcc agctcgcatt
      481 cttgatgttc atgttgttca tgggcagccg cgtcctgccc ccggcaaacg aattcgtgcc
      541 gacgctgctg ttcacgcccc acggactcgg gttgttggtt gcaggcaccg tggtcggcgg
      601 cgtgcttgcc accatcatct tttccatttc cgccatttcc gtaccgctcc tgctgacgcg
      661 cccggtcgat gcggtgacgg ccatggcggc gagtgtcgaa gccgtcgtga agaacccgaa
      721 gcccatggcc ctctgggccg ccctgattgc aagcttcatg gcgctcggga ccgcgtcgct
      781 cttcgtaggt ctcgcgatcg ccttcccgct catcggccat gcgacctggc acgccttccg
      841 cagcctcgtg gtgcagaacg aaccttgagg aaaaggtccc gaggccggac acctcatcag
      901 caattttccc ggtgcgaaac cgggcgcatc ccggcccgcg gtggttaccg gcaccgttcg
      961 gtgagggccc agtggtgaga cgcaaagcgc cccaggcgct catcgctcga tcgcttgccg
     1021 tatattcctg ctcagatccg caagcggatc attcccctcg aagcgatcgc gataaagcgt
     1081 cgcctgaacc agcagcagga aagtgaccgg ggtcgtcacc gtcaagaaag caccaatcag
     1141 aaattcgtgc aggacgaaac gcatctccgt catcgagaaa tacaacatcg accccgtcag
     1201 gatgctgaaa aggccgagcg tcgagccgag ggtcggcgca tggacgcgct cgtagaaggt
     1261 tttgaaatgc aataggccga ttgatccgat cagggccaga cccgagccga ggatcagcaa
     1321 gacagaaatc agcaacgctg cccagagcgg cagttcctcg atcgtcattc gatgacctcg
     1381 ccgcgcatga gaaacttggc catggccacc gtggctacga acccgagcag gccgatgacc
     1441 agtgccatct cgaaatagat cgtcgccccg gaaccgatac cgaaaaccag gagaagcagc
     1501 atggcgttga cgtacatcgt gtccaaggcg agcacgcgat cctgtgcccg cggtccccgc
     1561 agcatgcgat aggccgtgac tgccatcgcc agcgtcagga agatcttcgc tacgagcaac
     1621 gatatctcca ggattaatcc gctcattcga agatctccat cagtagccgt tcgtagcgca
     1681 tcttgatgat cttcccccaa tcgtcatcat cgaccaggtc cagcacgtgg atcacgagga
     1741 tattcgtctc ggggtcgtag gcgacccagc tcgtccctgg cgtagcggtg atgatgcacg
     1801 cgagggccgc aaggccgtat tgcgagtgca tcctgagcgg gatccaaacg aatccgggcg
     1861 tgcggccggg tctgtccgac aggatcagcg aggccacacg atagttggaa cgaacgatat
     1921 ccaccacgag ctcgcagcat agctgcagga cggccattgg ccgcctgacg atcaaccgtg
     1981 gcgtatcgag ccgcaccagg aaccacggac cgatgacgga cagcgctccg cccagcagaa
     2041 tgtgcgcgag agcgaggctc tgattgagca gcagccaggt cgcgagcagc aaaactgaaa
     2101 tgagcgggta aggcagccag gctttcattg tgtccgctcc ggctgctcag gtgcggtttg
     2161 ggccttcccg gtgaccgcgc tgatataagg ccgccccagc tccaacccat tggctgtcgt
     2221 ctgcatgaac cgcatcacgg gtcctgcttg gatggtaagg ccaacgcaga ggacaagcag
     2281 caacccgacg gcgccaagct cagtggcgcg cacatgcggc agctcgcggt cggtgaacgt
     2341 ccagaaggtc cgtatcccgg cacgcgtcac ggcgataacg gtcgtaagac cggagaccag
     2401 gatcaccgca agaagcagcc atccttgaat ggaaacgctg ccgccgacat tcagccagtg
     2461 ctggtcgagc aggttgctca agatggcgaa cttcgcgacg aacccggaga gcggcggaag
     2521 gccggcaagc agcaacgcgc aggcgagaaa agcgacgccc agcacaccca tgatggcggg
     2581 aaccgcgatg ccgacctcgc tttccacttc cgcatcctcc tcgtcgtccg caccgtagac
     2641 ctccaaggtc acggccaaga cgtcgtcagc gggcgtgcgg ccacgttcca cgagctcgac
     2701 gagcaggaag aaggcagcca ccgtcagcgt ggagctgacc agatagaaca gcgccgcgct
     2761 ggtcatgccc gcaccgccga cgccgatggc cgccaacacc gttccggacg agaccagaac
     2821 ggaatatccg gcaagacgcc ccatgtcctg cgatgccagc atcccgatgg cggcaaagga
     2881 tacggtcgcg ataccaatgg tgaacagccc gccttcggca aagaagagcg acgcgccgcc
     2941 gccggcctcg gccgtgatcg atcccagcct caggatgatg tagaggccga ccttggtcat
     3001 gatcgcaaac accgcggcca ccggagcgct tgctgccgca tacaagggcg gcagccagaa
     3061 gccgagcggc cacgccgcag ctttcaccag gaaggcgatg ccgaggatcg aggccccgat
     3121 ctcaaagagc ccccgctcgg aaggatcgag cgcggcaaca cgctccgcga gcgcggccat
     3181 gttgagcgtc cccgcggtgc catagatcac gctgacgcct atgaggaaga acgatgaagc
     3241 agcgaggttg atcacgatgt agtgcaggct cgagcgcaca cgtgcgcgcc ccgagccgtg
     3301 cagcgccaga ccataggacg ccgccaggag cacctcgaaa aacacgaaga gattgaacag
     3361 atcgcccgtg aggaacgcgc cattcagccc catgagaagg aactggaaca acgggtggaa
     3421 atagctcccg gcccggtgcc aggaggccaa ggcgaacagc agcgctgcac atccgagtat
     3481 tgccgtgagc agcaacatga gcgcggcgag cctgtcagcc accaggacga tgccgaactc
     3541 ggccggccag ttgccgagaa gatagaccct gacggcgcgg gcctggtcct cccccatact
     3601 ttggacgagc agcacgatag agaccgcgag cagcgccaag gtcgcaacga tgtcgatcgt
     3661 tgcaacgacc tcacgacgcc gcccctcgaa cagcagcatc aatccgcctg ccacgagcgg
     3721 aatgatgatc ggggctatga ccaggtgatc cgctgaaatc acgagccccg ctccactctg
     3781 tcgacgtgat cgctaccgga aagcccccga gcggcaagca agacgacgag aaacagtgcc
     3841 gtcatcgcga aactgatgac gatggccgtc aggaccagcg cctgcggtac cggatccgca
     3901 taacccgcga ggtaaggcgg gaccgtcgca tcgagcacag gcgccttgcc cacgcgcaga
     3961 cgtcccatac cgaagatgaa gagattgacc gcataggaga gcagcgagag cccgatgatc
     4021 acctggaacg tccgtggtcg cagaatgagc cacaaacccg atccggccag gatgccgatg
     4081 gttatggaaa tgacgagttc catcaggttg tttctccgac gggttgcgtc gcctcgctga
     4141 cgggacgtgg gatgcggagg gcctggtgcg cgagagcgat gaggacca
//
LOCUS       DASVDU010000274         3836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1032930, whole genome shotgun sequence.
ACCESSION   DASVDU010000274 DASVDU010000000
VERSION     DASVDU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032930"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..1707)
                     /locus_tag="VF226_18350"
     CDS             complement(367..1707)
                     /locus_tag="VF226_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023760734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEX7076004.1"
                     /translation="MRQERTVQGTLFDIFAEHEIGRELKAMSRWLDEHREVLSLAVAD
                     LRRHGLKDTGRHGLAAETVLRCALLKQHRQLSYEELAFHLEDSASFRAFARLPLGWSP
                     KKSVLQRTISGLGAVLWETINRAMMDSARRHNVEDGSLVRIDSTVTAALMHAPTDSSL
                     LWDAVRVMVRILKKADELLGGTLPRWRNCRRLAKKRAVAIQYIRGQRQRAELYRDLVA
                     ATRATMTSLRCAVQHLLVHPSLEAVALRCEAEHYLPLIARIIDQTERRVFAGEAVPAD
                     EKLVSLFETHADIIVKGGRDTHYGHKLNLTTGRSGLILDVVIEAGNPADSSRFLTMLE
                     RHVAHHDAPPRQTSADGGYASTANLKRAKALGVEDVAFHKKCRLAVEDMVKNRQMYRK
                     LRNFRAGIEANISCLKRAFGLRRCTWRGLAHFHSYVWASVVAYNLALFTTLAQP"
     gene            complement(1864..2142)
                     /locus_tag="VF226_18355"
     CDS             complement(1864..2142)
                     /locus_tag="VF226_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076005.1"
                     /translation="MSRADKGFTEKQGQYLAFIYTYSHMFRRAPAETDMQRHFQVSPP
                     SVHQMVVTLERAGLIRRQPGVARSIELLVAPEDLPILKWLEINPSKSL"
     gene            2197..3315
                     /locus_tag="VF226_18360"
     CDS             2197..3315
                     /locus_tag="VF226_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7076006.1"
                     /translation="MNVRYRVELSQAERCELSAMLSKGKQAARKLKRAQILLAADAGN
                     SDEAIADTLSVSGSTVYRTKRRFVEGNLERALSEEPRPGAERKLTVKEEALLVATACA
                     KPPEGRARWTLELLAGEFVKLTDHDSLSGETVRRRLAENGLKPWRRDMWCIPKVDGEY
                     VARMEDVLDLYAEAPDPKRPVVCFDESPVQLIGEVRQPIPATPGQLERYDYEYRRNGT
                     VNLFVCLDVNRPWRKVKVTARRAAEDYAQCLRELVDVHYPHAECIRVVQDNLSTHSAG
                     ALYEAFAPAEARRILRRLEFHYTPRHASWLNMVEVEIGVLRGQCLDRRIDDPTWLAKE
                     VAAWEQQRNTAGARIKWMFTTDKARAKMSRAYPALKES"
     gene            complement(3446..>3836)
                     /locus_tag="VF226_18365"
     CDS             complement(3446..>3836)
                     /locus_tag="VF226_18365"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7076007.1"
                     /translation="HVSGNKTLIPDDGVKRFRVLTLMALGQAIAEAGVQQRYETAMRP
                     QGLQWREWMDRQGLRVSAACDDLETTWEKDLDEVTAGSIAVAVALAYLDFRVPDWQWR
                     KGRPKLHAFHEGFSARPSMQATLLKAA"
BASE COUNT          772 a         1172 c         1184 g          708 t
ORIGIN      
        1 ccggcacacg acgatcgaga acagccaata ttggatcgga ccgcagatca tcgtggcgca
       61 agcgatcaag agccgcttcc gcggccacgg ccgcaattgg gatttcaagg gccgctcggc
      121 cgagatctgc gccctcgatt ggaaggggcc ttagttagtg tctgtccata aacgaagatt
      181 ttttggcgag cgttgtaacg gatgcagtgt tttcaggggt tagcgcggca tgctggagtc
      241 agggtcgccg atggaatgag ctgatttttt gaagggaacg aacgtaagag gagtttcgcc
      301 gggagcggag acgtgaggag gcattcgccg gatggggcgc tggcggcgct cgacgatttt
      361 gttgcttcag ggctgagcga gcgtggtgaa caaggcgaga ttgtaggcca ccaccgatgc
      421 ccagacatag gagtggaagt gcgcgagccc ccgccatgtg cagcgccgca atccgaatgc
      481 gcgcttcagg cacgagatgt tggcctcgat gccggcgcgg aagttccgca gcttgcgata
      541 catctgccgg ttcttgacca tgtcctcgac cgcaaggcga cactttttgt ggaaggcgac
      601 atcctccacg ccgagcgctt tggcccgctt gagattggcg gtgctggcat agccgccgtc
      661 ggccgaagtt tgcctcggcg gcgcatcatg gtgcgcaaca tggcgctcca gcatcgtcag
      721 gaaccgagag gaatcggctg gattgccagc ctcgataacg acgtcgagga tcagtccact
      781 gcggccggtc gtgaggttga gcttatggcc atagtgagta tcacggccac ccttgacgat
      841 gatgtcggcg tgcgtctcga acaggctgac cagtttctca tccgccggca ccgcctcgcc
      901 ggcgaacacg cggcgctccg tctgatcgat gatgcgggcg atcaaaggca aataatgctc
      961 ggcctcgcaa cgcaacgcca cagcttcgag gctgggatgc accaataaat gctgcacggc
     1021 gcagcgcagc gacgtcatgg tggcacgggt ggcagcgacc agatcacgat aaagctcggc
     1081 cctttgcctt tggccacgga tgtattggat cgcgacggca cgcttcttcg ccagacggcg
     1141 gcagttacgc cagcgcggca gcgtgccacc caacagctcg tcggctttct tcagtatccg
     1201 taccatcacc cgaaccgcgt cccacagcag cgagctgtcg gtcggggcat gcatcaacgc
     1261 cgccgtcacc gtgctgtcga tccgcacaag cgatccatct tcaacgttgt gacgccgtgc
     1321 gctgtccatc atcgcgcggt tgatcgtctc ccaaagaacc gcgccgagcc cactgatggt
     1381 tcgctgcagc acggacttct tcggcgacca gcccagcggc agccgggcaa aagcgcggaa
     1441 cgacgccgaa tcctccagat gaaacgccaa ctcttcataa ctcagttgcc gatgctgctt
     1501 gagcagagcg cagcgcagca ccgtctccgc cgcaaggcca tgccgccccg tatccttgag
     1561 gccatgtctg cgaagatcgg caaccgccaa actcagcact tcacgatgct catccagcca
     1621 ccgcgacatc gccttcagtt cgcgaccgat ctcgtgttcg gcaaaaatat cgaatagagt
     1681 gccttggacg gtgcgttcct gacgcattgt tggctccggc gattgagggg tgatttgctt
     1741 cggtggtacg atccgaatca tacccgaaat cgccgggctc aacgccgcca aaatcagatt
     1801 ccacaaataa tatcagtcgt ttgttgaacc ctcagtcctt tatggacaga cactagtacc
     1861 tcgtcacagt gattttgacg ggttgatttc gagccatttt aggatgggca ggtcttcagg
     1921 agcaacgagg agttcgatgc ttcgggcgac gccgggttgg cggcgaatga ggccggcgcg
     1981 ctcgagcgtg acaaccattt ggtggaccga aggcgggctg acctggaagt ggcgttgcat
     2041 gtcggtttcg gctggggcgc gccggaacat gtgcgaatag gtgtagatga aggccagata
     2101 ctggccttgc ttttcggtga agcctttatc ggctcgactc attcctgaca tccgattcag
     2161 tcgtttgttt gtgcctcgaa cctgaaggag gcacggatga atgtacgtta tcgggtcgag
     2221 ctgagccaag ccgaacgctg cgagctcagc gcgatgctga gcaaaggcaa gcaagccgcc
     2281 cgcaagctca agcgcgccca gatcttgctg gcggcggatg ccggcaacag cgacgaggcg
     2341 attgccgaca ccttatcggt gagcggttcc accgtgtacc ggaccaagcg gcgcttcgtg
     2401 gaaggcaatc tggagcgggc gctgagcgag gaaccgcgtc cgggagcgga gcgcaagctg
     2461 acggtcaaag aggaagccct gctcgtggcg acggcctgtg cgaagccacc ggaaggccgc
     2521 gcccgttgga cgctggagct cttggccggt gagttcgtca agcttaccga ccacgacagc
     2581 ctgtcaggcg agacggtgcg ccggcggctg gccgagaacg gcctcaagcc gtggcgccgg
     2641 gacatgtggt gcatccccaa agtcgacggc gaatacgtcg cgcgcatgga agacgtgctc
     2701 gatctctatg ccgaagcgcc cgatcccaag cggcccgtag tatgcttcga cgagagcccg
     2761 gtccagctca tcggggaagt gcgccagccc atcccggcca cgccgggcca gctcgaacgc
     2821 tacgattacg aataccgccg taacggcacc gtcaatctct tcgtctgtct ggatgtcaac
     2881 cggccttggc gcaaggtcaa agtcaccgcg cgccgggccg ccgaggacta tgctcaatgc
     2941 ctgcgcgaac tcgtcgatgt ccactatccc catgccgaat gcatccgggt cgttcaggac
     3001 aacctgtcga cccactcggc gggcgccctg tatgaagcct ttgcgcctgc cgaagcccgc
     3061 cgtattctgc gccgcctgga attccactac acacccaggc acgccagctg gctcaatatg
     3121 gtcgaggtcg agatcggggt gctgcgcggc caatgcctcg accgcaggat cgacgaccca
     3181 acatggctcg ccaaagaagt ggccgcctgg gaacaacagc gaaacaccgc cggcgctcgc
     3241 atcaagtgga tgttcactac cgacaaagcc cgcgccaaaa tgagccgcgc ctatccggcg
     3301 ctcaaagagt cataatcact gtgacgaggt actagttagg gcgcatcgcg gcgaagtgga
     3361 atcacttcgc cgaaaaggat ccgcgccaat caatatgttg gaacaatcct catcgccaag
     3421 gtcttcaact ctggcgggat ctgccctaag cggccttcaa cagggtggcc tgcatcgacg
     3481 ggcgcgccga gaatccctca tggaaggcgt gtagcttcgg ccggcccttg cgccactgcc
     3541 agtcggggac gcggaaatcg agataggcga gggcgacggc gacggcgatc gagcccgcag
     3601 tcacctcgtc gaggtccttc tcccaggttg tctcgagatc gtcgcatgcg gcgctcaccc
     3661 gcaagccctg gcggtccatc cattcacgcc actgcagccc ttgcgggcgc atcgccgtct
     3721 cgtagcgctg ctggaccccg gcttccgcaa tggcctggcc cagcgccatg agggtcagca
     3781 cccggaaacg cttcacgccg tcatcgggga tcagcgtctt gttgccagac acatgg
//
LOCUS       DASVDU010000275         6192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1225688, whole genome shotgun sequence.
ACCESSION   DASVDU010000275 DASVDU010000000
VERSION     DASVDU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225688"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /gene="lptD"
                     /locus_tag="VF226_18370"
     CDS             complement(<1..1590)
                     /gene="lptD"
                     /locus_tag="VF226_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX7076008.1"
                     /translation="MRRARTPQDRSAQRRATIRRLLAGGGFCALAISLLLVLQSPAGA
                     QDTSVVAPSIAAMPNTLQQPKAPSAFSKNKNGNPLFGPVAPVDAKQPLYLQGDELIYD
                     SEGNRIIARGNVEIYFNNYILLADEVIYDQGANTLSAVGNVTLKEPNGLITRGERITL
                     TDDFRDGFVQSLSVTARDDTRIAAERAIRREGNITEFQNGRFTPCKPQGDMPPLWCIS
                     AARVVHDQAAATISYQDAWFELFGVPIIGLPYFQHADPSVKQKSGFLTPSYLNSEDLG
                     FGIETPYYFALAPNYDFTFHPMYLTRQGILWQGDWRHRLSFGSISGMYTVKFAAIDQD
                     ASQLPGTAADNERFDGWRGSIETKGRFSLSSWWNFGWDVTLESDDYFRRFYKLDNILQ
                     SDRINQVYLTGLSERNYFGAYLYHFGGLMFEDTSVSEGRVHPVIDYNYIFADPILGGE
                     LSFNANALSFSRDYSKVEGPGESAMNRVVAEVNWRRRFTDPLGQTYTPFANLRGDIYT
                     LHDVVDPVTLDVIEDEAVTRGV"
     gene            complement(1595..2683)
                     /gene="lptG"
                     /locus_tag="VF226_18375"
     CDS             complement(1595..2683)
                     /gene="lptG"
                     /locus_tag="VF226_18375"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX7076009.1"
                     /translation="MRGLTLKLYIARHFLIGILGTFAVCALLIFMIDFVEVLRQSGKH
                     GSVPATTLVWLTLLRLPAYTEILLAFAVLVGSIACLIHLSRRSELAVIRAAGMSVWQF
                     ISPGIVVAFILGVLAVAFYNPLAAKARAEAEELFAEVFGKESNFLKTRAGSWLRQDGA
                     DGSSVMNAGGVADGGLKLFTVTVFQFDREGRFVERIEAKRATLRDGYWELEGVRVAGA
                     GRAPETYDRYLISTYLTPERVQDALGSVISLSFWDLPGLIEVAERAGLSAAPYRVQYE
                     LLLSRPLLLICMVLFAATVSLRSFRSGGIQTMVATGMIGGLGFFLLAEVSRQVGVAGL
                     VSPKVAVWAPVAVAALVSLTVLLHQEDG"
     gene            complement(2690..3901)
                     /gene="lptF"
                     /locus_tag="VF226_18380"
     CDS             complement(2690..3901)
                     /gene="lptF"
                     /locus_tag="VF226_18380"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX7076010.1"
                     /translation="MSTFSRYLFRQVAGALLLILLSLSGVVWIALALRELDLVTSEGQ
                     DALVFVIMTTLALPNLIALIAPIALLIATIHTLNRLNGDSELIVLTASGATVWTIARP
                     LLLLALLVSIGVAFVNHIGMPWSLRLLREYVVQVRADLISQVLKPGQFSSPERNLTFH
                     IRDRTYDGELLGILMHDARNKEQTMSYLAERGTLVKQGEATFLVMSDGHIVRQPQNGD
                     PPQIVTFESYAVDLDRLEPKGSGSTVLKPRERYFSELVRPDPEDPYLKHRPGEIRAEL
                     HERLSNPLYPVAFVLIAVAFIGQARSTRQNRVEALVLAFVLSAVSRIGGLAANNLAVI
                     HASAVPLLYLMPLGGIGLAIALMSLNARPRGGPGVTERLRQAATFVLSGAAPLLPARL
                     GPRASRREETG"
     gene            4248..5777
                     /locus_tag="VF226_18385"
     CDS             4248..5777
                     /locus_tag="VF226_18385"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7076011.1"
                     /translation="MSERLAIAFAPFKAPLQPVAALLCAEELALGPETRSLDRKANGM
                     VLRAAEAAAFKGKAKTSFELLAPAGLDVSRLIVIGAGQPDQAKETDWVNLGGYLYAQI
                     AARKSEEASVVADLPDPRSRSAADIAADIAFGALLRSYRFNKYTTRQKDENGEEKKND
                     GLARLVVHTQDPEAAEAAFARRKAVANGMFLARDLVNEPANMLGPVDFAERVKELTSL
                     GLEVEVLDVDALKELKMNALLAVGQGSARPPRVVVLQWNGARSRRSKPLCFIGKGVCF
                     DTGGLSMKPAQGMEDMKGDMGGAACVAGLMQALAERRANVNAVGILGLTENMPSGTAQ
                     RPGDIVTSMSGQTIEVLNTDAEGRLVLADVLWYAQERFKPRFMVDLATLTGAIMIALG
                     KEYAGLFANDDKLAADLIEAGTATGEQLWRMPLARAYDKLIDSRIADMKNIGGRHGGA
                     ITAAQFLQRFVKDTPWAHIDVAGVAMDSPKTDISQSWASGWGVRLLDRMIADKYERTE
                     K"
BASE COUNT         1171 a         1926 c         2025 g         1070 t
ORIGIN      
        1 aacgccacgc gtcacggcct cgtcctcgat cacgtcgagc gtgacggggt cgacgacgtc
       61 gtgcagcgtg tagatgtcgc cccgtaggtt ggcgaacggt gtgtaggtct gcccgagcgg
      121 atcggtgaag cggcgccgcc agttcacttc ggcgacgaca cggttcatgg cggactcccc
      181 aggtccttcc actttcgagt agtcgcgcga gaagctcaga gcattggcat tgaacgacag
      241 ctcgccgcca aggatcgggt ccgcaaagat gtagttgtaa tcgatgacgg ggtgtacacg
      301 cccttccgaa actgacgtgt cctcgaacat caaaccaccg aagtggtaga gataggcgcc
      361 gaagtaattc cgctcgctga gacccgtcag gtagacttgg ttgatccggt cagactgcag
      421 gatgttgtcg agcttgtaga accggcggaa gtagtcgtcg ctctccagcg tcacatccca
      481 cccgaagttc caccaggagg agagcgagaa ccggcctttc gtctcgatgc tgccacgcca
      541 cccgtcaaac cgctcgttgt cggcagccgt gcccggaagc tggctggcat cctgatcgat
      601 cgcggcgaac ttcaccgtgt acattccact gatgcttccg aacgacagcc ggtggcgcca
      661 atcgccctgc caaaggatgc cctgtcgcgt caggtacatc gggtggaagg tgaagtcgta
      721 gttcggcgcc agcgcgaagt agtagggtgt ctcgatgccg aacccgagat cctccgagtt
      781 gaggtaggac ggcgtcagga agccggactt ctgcttcacc gagggatcgg cgtgctgaaa
      841 atagggaagc ccgatgatcg gaacgccaaa gagctcgaac caggcgtcct ggtaggagat
      901 cgtcgcagcg gcctggtcgt gaacgacgcg tgcggcgctg atgcaccata gcggcggcat
      961 gtcgccctgc ggcttgcagg gggtaaatcg gccgttctgg aattccgtga tgttcccttc
     1021 ccggcgaatc gcgcgctcgg ccgcgatccg cgtgtcgtcg cgcgcagtga cgctcagcga
     1081 ctgcacgaag ccgtcccgga agtcgtcggt gagcgtgatg cgctcgccgc gagtgatgag
     1141 gccgttcggc tccttcaggg tgacgttgcc gaccgcactc agcgtgttgg cgccctggtc
     1201 gtagatgacc tcgtccgcca ggagaatgta gttgttgaag tagatctcga cgttgccgcg
     1261 ggcgatgatg cggttgcctt cactgtcgta gatcagctca tcgccttgga gatagagggg
     1321 ctgcttggcg tcgaccggcg ccaccgggcc gaagagcgga ttgccattct tattctttga
     1381 gaatgcggac ggcgctttgg gctgctgcag ggtgttgggc atggccgcaa tcgatggggc
     1441 caccacgctc gtatcctggg cgccagcggg actttgcagg accaagagca ggctaatcgc
     1501 gagcgcgcaa aagccaccgc ccgccaacag gcggcggatc gtggcccgtc gctgggcact
     1561 acgatcttgc ggcgtccgcg ctcgacgcat cacgtcaacc gtcctcctgg tgcagcagca
     1621 ccgtaagtga caccagggcg gctacagcaa ccggcgccca caccgccacc ttcggcgata
     1681 cgagtccagc aacaccaacc tgtcgggata cctctgccag cagaaagaac cccagccccc
     1741 caatcattcc tgtcgcaacc atagtctgaa tgccgcccga ccggaatgac cgcaacgaca
     1801 cagtggcggc aaaaaggacc atgcatatca atagcagagg ccgagacagc aggagctcgt
     1861 actggacgcg atagggcgca gcggacaggc ccgcccgttc ggccacctcg atcagaccag
     1921 ggaggtccca gaatgacagc gagatgacgc tgccgagcgc atcctgcaca cgctcagggg
     1981 tcagataggt gctgatgagg tagcggtcgt aggtttcggg cgcgcgaccc gcaccagcca
     2041 cacgcacacc ctcgagctcc cagtatccgt cgcgcaacgt ggcccgcttg gcctcgatgc
     2101 gctcgacgaa ccggccctcg cggtcgaact ggaacacggt gaccgtgaac agcttgaggc
     2161 cgccatcggc gacaccgccg gcgttcatca ccgaggagcc gtccgcaccg tcctggcgca
     2221 accaggaccc ggcacgggtc ttcaggaagt tcgactcctt gccgaagacc tccgcgaata
     2281 gctcttcggc ctcggcgcgc gcttttgccg ccaaggggtt gtagaaggcg accgcaagca
     2341 cgccgaggat gaaggcgacg acgatacctg ggctgatgaa ctgccacacc gacatgccgg
     2401 ccgcgcggat cacggcaagc tcgctgcggc ggctgaggtg gatgaggcat gcgatgctgc
     2461 cgacgagcac ggcgaaggcg agcaggatct cggtgtaggc aggcaacctc agcaaggtga
     2521 gccagacgag cgtggtggcc ggtacgctgc catgcttgcc ggactggcgc aggacctcga
     2581 cgaagtcgat catgaagatc agtagcgcgc agacggcgaa cgtgcccagg atgccgatga
     2641 ggaaatggcg cgcgatgtag agcttgagcg taagtccccg catgtgaggc taccccgttt
     2701 cctcgcggcg cgaggcacgc ggtcccaatc tggccggcag cagcggcgct gcgccgctca
     2761 gaacgaatgt agcggcctgc ctgagccgct ccgtcacccc cggcccgcca cgcggacgcg
     2821 cattgaggct catcagcgcg atcgccaggc cgatccctcc gagcggcatt agatagagca
     2881 ggggcacggc gctggcgtgg attaccgcca gattgttggc cgccagaccg ccgatccggc
     2941 tcaccgccga gaggacaaag gccagcacca gcgcttcgac ccggttctga cgcgtggagc
     3001 gtgcctgccc gatgaatgcc accgcgatca gcacgaacgc gaccgggtag agcggatttg
     3061 acaaccgctc atgcaactcg gcccggatct cacccgggcg atgcttcaag taagggtctt
     3121 ccgggtcagg cctgaccaac tcgctgaaat accgctcgcg cggcttcagg acggttgacc
     3181 cgctgccttt cggctcgagc cggtcgaggt cgacggcgta gctttcgaat gtgacgatct
     3241 ggggcgggtc gccgttctgc ggctggcgga cgatatgacc gtccgacatg acgaggaaag
     3301 tggcttcgcc ctgcttcacg agtgtgccgc gctcggccag gtacgacatc gtctgctctt
     3361 tgttgcgggc atcgtgcatg aggatgccga gcagctcgcc gtcgtaggtg cggtcccgaa
     3421 tatggaacgt gaggttgcgt tccgggctcg agaactgccc cggcttcaac acctgtgaaa
     3481 tcaggtcggc ccgcacctgg accacatact ccctgagcaa ccggaggctc cagggcatgc
     3541 caatgtgatt gacgaaggcg acgccgatgc tgaccagcag cgccaggagg agcagcggcc
     3601 gcgcgatcgt ccacacggtg gcgccggagg cggtcagcac gatcagctcg ctgtcgccgt
     3661 tgagccggtt gagcgtgtgg atcgtggcga tcagcagcgc gatgggggcg atgagcgcaa
     3721 tcagattggg cagcgccagg gtcgtcatga tcacgaagac caaggcgtcc tggccctcgc
     3781 tggtcaccag gtcgagctcg cggagcgcaa gcgcgatcca cacgacgccc gaaagcgaca
     3841 ggaggatcag cagcagggca ccggcaacct gacggaagag gtaacggctg aaggtgctca
     3901 tggctcgccg gaatcccgag gctgctcaac gtccttgccg gaatcgatca ccgccgtcct
     3961 gctctttccg tgctccgccc gttcgctcgc cgtctcatag ttcggtggcc aaaattcggc
     4021 tcccccggcg aagcccgcgc tcggagtcct cgcttgcact tcctgcgcga gcggctaatt
     4081 tcgcacagga attgcgccag cgccgatctg ctgccagccg ttatgagcgg ggctctaggg
     4141 attcgcaacc tcgggttcgg atcggggttg tgcgtcgtcc atccagccca agcaaaagcc
     4201 ggttggcgaa ttctcgatca tcgcattgga tcagcccagg atcacacatg tcggaacgac
     4261 ttgccatcgc gttcgcgccg ttcaaggcgc ccttgcaacc tgtcgcggct ctgctctgcg
     4321 cagaggagct cgcgttggga cctgagacgc gctccctcga caggaaggcc aatggcatgg
     4381 tgctgagggc ggcggaggcg gctgcgttca agggcaaggc gaagacatcg ttcgagctgc
     4441 tggctccggc cggtctcgac gtcagtcgtc tgatcgtgat cggcgccgga cagccggacc
     4501 aggccaagga gaccgactgg gtcaacctcg gcggctatct ttatgctcag attgctgcac
     4561 gcaagtctga ggaggccagt gtggtcgccg atctgcccga tccgagaagc cggtcggctg
     4621 cggacattgc ggcagacatc gcattcggcg ctctgttgag gagctatcgc ttcaacaagt
     4681 acacgacccg ccaaaaggac gagaacggag aggagaagaa gaacgacggc ctcgcccggc
     4741 tcgtcgttca tactcaggac cccgaagcgg ccgaggctgc ctttgcccgc cgcaaggccg
     4801 tcgccaacgg gatgttcctg gcgcgcgatc tcgtgaacga gccggcgaac atgctggggc
     4861 cggtggactt cgcagaacgg gtcaaggagt tgaccagcct cggcctcgag gttgaggtcc
     4921 tcgacgtcga tgcgctcaag gagctgaaga tgaacgcgct ccttgccgtc gggcagggga
     4981 gcgcgcggcc tccgcgcgtc gtggtcctgc aatggaacgg cgccaggtcg cggcggtcga
     5041 agccgctgtg cttcatcggc aagggcgtct gcttcgacac cggcggtctc tccatgaagc
     5101 ctgctcaggg catggaggac atgaaggggg acatgggcgg cgctgcctgt gtcgccgggc
     5161 tgatgcaggc ccttgccgag cggcgggcga acgtcaatgc cgtcggaatc cttgggctca
     5221 cggagaacat gccgtccggc acggcacagc ggcctggtga tatcgtcacc tcgatgtccg
     5281 gccagacgat agaggtgctg aataccgacg ccgaaggccg gctggtgctg gccgatgtgc
     5341 tctggtatgc gcaggagcgc ttcaagccgc gcttcatggt cgacctcgcg acgctgaccg
     5401 gcgcgatcat gattgcgctg ggcaaggagt atgcaggact cttcgccaac gacgacaagc
     5461 tggccgcgga tctgatcgag gccggcaccg ctacgggcga gcagctctgg cggatgccgc
     5521 tggcacgggc ctacgacaag ctgatcgact cgcgcatcgc cgacatgaag aacatcggtg
     5581 gccgacacgg cggggcgatc acggctgcac agttcctcca gcgcttcgtc aaggatacgc
     5641 cttgggcgca catcgacgtg gcaggtgtcg cgatggattc gcccaagacc gacatcagcc
     5701 agagctgggc ttccggctgg ggcgtccggc tgctggatcg gatgatcgcg gacaagtacg
     5761 agcgtacgga gaagtaaggc ggctgacgcg cgcccgggct tagggcgcgc cgatctttat
     5821 tgtatcgctt tgagttgtct cacaaatttc tctctatttt ccataatata gattatgcga
     5881 atgagagcga ttggcgggaa attcctcagg gctccccacg aacgccaacc ctgggcggat
     5941 ccgcatcgcc cagcctccgc ctgtcaaccg cctgtaaact gaggtcggtg agctgaaggc
     6001 ggctcactga cgccgtcgcg ccgcggccgc cggcgcaccg caactcggtc gctctttccc
     6061 gcggctcatc ttggttaatg gcccacggac gagtcgcgtc gacctcctgc gatcggacga
     6121 tcacgcgctg ctctttgtgg caggctgcca aaggagcgca tgcaactttt ttcgcgttca
     6181 ccccggacca tc
//
LOCUS       DASVDU010000276        20838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1041971, whole genome shotgun sequence.
ACCESSION   DASVDU010000276 DASVDU010000000
VERSION     DASVDU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20838
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041971"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF226_18390"
     CDS             <1..243
                     /locus_tag="VF226_18390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7076012.1"
                     /translation="VVPHDELDAEVARWCAEILEHSPTAIAIAKRSFNADTEHIRGIG
                     AMGMQTLKMYYQTEESKEGVRAFIEKRKPDFRKYVK"
     gene            283..1443
                     /locus_tag="VF226_18395"
     CDS             283..1443
                     /locus_tag="VF226_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7076013.1"
                     /translation="MPIDKDSFDEFLGTIRRFVRERLIPNEARVAEDDAIPDDIVADM
                     KSLGLFGLAIPEDYGGLGLSMEEEVLVIFEIARASPAFRSYFATNVGIGSQAIVMDGT
                     EEQKRRYLPRLASGEIIGSFALTEPNSGSDAASLQTTARRDGDFYVLNGTKRFITNAP
                     EAGVFTVMARTDPDKKGADGISAFVVDRDTAGLTVGKPDRKMGQRGAHTADVIFENCR
                     VPRDAVIGGVEGQGFKTAMKVLDKGRLNISAACTGAAERILSDALAYALEREQFGKPI
                     AEFQLVQAMLADCQTEILAARSMILEAARKRDRGGDVTMEASCCKLFASEMVGRVADR
                     AVQIHGGAGYMAEYAVERFYRDVRLFRIYEGTSQIQQLVVARNMIRKARAEA"
     gene            1517..2254
                     /locus_tag="VF226_18400"
     CDS             1517..2254
                     /locus_tag="VF226_18400"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7076014.1"
                     /translation="MNDSQQQPAEHDLFGRLRSLKGVRLEWPILLIVLIVASAIWGFI
                     ELADEVSEGSTDAIDRRLLLLLRDPNDPFKMLGPSWLGELMRDFTSLGGTGVLTFVTL
                     SATGFLVLERKRHAAALLLVSVVGGVILSNVLKLTFARPRPEFSDYEPLIATASFPSA
                     HSMMSAVVYLTIGALIARMRPDPLVRAYVLSLAIFVAVIVGISRIYLGVHWPTDVLAG
                     WVVGSAWALFCWLVALLLQRRGDVENP"
     gene            complement(2284..2700)
                     /locus_tag="VF226_18405"
     CDS             complement(2284..2700)
                     /locus_tag="VF226_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7076015.1"
                     /translation="MVAFLAASDAYAESLYPPESNHMVDLAALMAPNTVFFVARQGEK
                     ALGCGALIIGEDGSGEVKRMWVDPEARGQGIGHKILDAIEGAAREKGLTILRLETGIR
                     QPEAIALYRKRGFNECAPFGSYLADPLSLFMEKRLA"
     gene            complement(2890..3267)
                     /locus_tag="VF226_18410"
                     /pseudo
     CDS             complement(2890..3267)
                     /locus_tag="VF226_18410"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
     gene            complement(3260..3820)
                     /locus_tag="VF226_18415"
     CDS             complement(3260..3820)
                     /locus_tag="VF226_18415"
                     /inference="COORDINATES: protein motif:HMM:NF023177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HEX7076016.1"
                     /translation="MSSAISVPVGVVVAREKIDHPWQEYVWRAVDVFLHPPEISGWRE
                     LRRDERSVYYHAATLPIELHRKETPGYKTNLETGEPAVYVVLRNDPGADSDWPVTVHM
                     VTASPHDAQAYAESGLETIELLPMPQLLIDLVREFVDAHHVEERFVKRQRQKFEIEEE
                     HRFGQEPIFVLRERMRARRRGEGGDD"
     gene            3950..5998
                     /locus_tag="VF226_18420"
     CDS             3950..5998
                     /locus_tag="VF226_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7076017.1"
                     /translation="MSGTKPKLLVCNCQRTMDIDGARLAKMLGLEEPLKVHRELCRAE
                     IASFESALEAGPVHVACTQEAPLFRDLAEDKGLAERALRFTNIRERAGWSEAKSAALP
                     KMAALLAESAFVPKPAGLITLKSEGVCLVYGPGQIALDVAQELGSRLSVSVLLMTDAA
                     DAIPPSIADVPIHRGRIRKATGYLGAFEIEVDGYAPMLPSSRSTLEFVMARDGAKSQC
                     DIILDLSGGQPLFSDGGRRDGYVRADPSNPAAVAKAMFTVSDLVGEFEKPRYVEVDTA
                     ICAHSRSQKIGCTNCLDSCQTGAISPAGDHVVIDAGVCAGCGTCSAVCPTGAVSYKFP
                     TREDLITRAQVLLKAYRTAGGTRPILLLHDEKHGSPIISAIARFGRGLPANVIPLSLY
                     SVFQLGHDVLASMLASGAEQLVILASGQAPGELPPLESQVELLGAFLEALGHQGPRAR
                     LVVEQDPDAVESLLYDLPALVELTPRFFSTAGSKREVARTALATLNELAPQPATVIPL
                     PKGAPYGRVHIDVEGCTLCLSCVSACPANALSDHPDRPQLSFTEAACVQCGVCVATCP
                     ENVIRLEPRYNFTPSAMTPEVLKGEEPFECISCGKPFGARATIEKVIERLKGHSMFRD
                     EEQLRVIQMCDTCRVVAVAESSDDPFKFGERPRMRTTDDDIAEAERARAMRKPEDFLS
                     "
     gene            complement(6083..7093)
                     /locus_tag="VF226_18425"
     CDS             complement(6083..7093)
                     /locus_tag="VF226_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HEX7076018.1"
                     /translation="MRDFWKSAGFHLVEIGAEGWLKVTPQLLLAYWTRPEVHPIETSC
                     DEEIRLHEELLADPLMPVDEARLARLADPDTADNYRLVLAFRELLMSAGTIEGAYLRI
                     ARGTNTTVPPVFVDQMVHLILRNILKDVRDPMRLRAAELFFREQIVSTEGGRLLLADE
                     EIVEMHGSSGETGLAQLLAETGTPMRTVELDVLSEDNKEIYWERSDRFDTVIDFRFEQ
                     PALDAFARVLEAWLQHMMRLEVRVEPRPKVEDSDWRWHIGLDREATQILNALYEGKPV
                     GADDLARIVALFRMHVRDDRTVIDRVKGRPIYLGLAMTPANRLKMKPQNLLVNMPLRM
                     AA"
     gene            complement(7093..7575)
                     /locus_tag="VF226_18430"
     CDS             complement(7093..7575)
                     /locus_tag="VF226_18430"
                     /inference="COORDINATES: protein motif:HMM:NF040448.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6505 family protein"
                     /protein_id="HEX7076019.1"
                     /translation="MKLLRTIRFDSSDDRVFEHAAASDEWAVSGAFAFAHLAPADVTG
                     KVKQAFANGFLGVESFGRSTFATVAEADQAAADEIIFRLACHFVEAYGAPDIDAAWPA
                     AQEELAFILDLCRVAPINTVFTVRRTFDPEGQIKEEFRTIKAPTGEPMHARIWTVEPD
                     "
     gene            complement(7572..8342)
                     /locus_tag="VF226_18435"
     CDS             complement(7572..8342)
                     /locus_tag="VF226_18435"
                     /inference="COORDINATES: protein motif:HMM:NF028227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HEX7076020.1"
                     /translation="MAEAPYMHLPEPVFPPLLAGHDVKAPLRPFSHACRQASRGTLGA
                     GDLVWSRNTSRAECAIVLEPEVPLGTALQMNALAQVALADSLGALLPPAVCVWHRWPG
                     TILVNGAECGGVVVGLPATEAGQVPDWMVVGMSLRLVHDDPGKEPGELPGQTALAEEG
                     GGEITRTDLLQCYASYLLSWLNTWQEDGFRPIHDSWMARAEADGKAMEVEHEGTRQVV
                     QVLGLDEEGGLLIRPEAGPARVLPLIEHIEVYGRELAR"
     gene            complement(8335..8541)
                     /locus_tag="VF226_18440"
     CDS             complement(8335..8541)
                     /locus_tag="VF226_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX7076021.1"
                     /translation="MTDKEPEDRFNISLRKFLKHVGVTSQREIERIVREKQLSGQGPL
                     KVRMELTAEGTDLKHVVEGEIEVG"
     gene            complement(8559..8681)
                     /locus_tag="VF226_18445"
     CDS             complement(8559..8681)
                     /locus_tag="VF226_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076022.1"
                     /translation="MRAFLGSLALLVVISALAAVGLNYMSMSVQDTYTTQNVRL"
     gene            complement(8695..10224)
                     /locus_tag="VF226_18450"
     CDS             complement(8695..10224)
                     /locus_tag="VF226_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7076023.1"
                     /translation="MAQAVRAGRYAADRASFACRPQRAAPPTGPIGCLAILLLILAGA
                     IGTAAAQDARALPPGGSPAAGQKSADERRAAAGNLVGHGGPIKAVRIDTASNRVLTGS
                     FDYAMMAWDISGEQPRQLARFEDHDGAVNAVAFVPGTSRVLSAGDDGTVSIWDISTGA
                     LVHRFSGHQGKIVGLAVTGDGHWAASASWDHTARLWDLEQLAPGPVLRGHAGPVNAVA
                     FSADGSRVYTASYDGSIGVFRREDGSFERPVYRHGWGINVLERLPDSERLVFGAINGA
                     AGVIDPGNGKVVVQLKQHERPVLAVAVLDKPSLIATGSADGVIRVSRSGDGAVLEEYQ
                     NTYGPIWALAFTREGRSLYYGGLDDFATLWHIRPRQPFEPVQSEFPRRFQQVGESDDV
                     LEQGRIQFARKCSICHTLTPDGGNRAGPTLYNLFGRRIGTLPGYPYSEALKQLDIVWT
                     EETVSKLFELGPDEFTPGSKMPLQKMTDKAQRDALVAYLKVATAPPSVDGGTGTGDGS
                     K"
     gene            complement(10234..11400)
                     /locus_tag="VF226_18455"
     CDS             complement(10234..11400)
                     /locus_tag="VF226_18455"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX7076024.1"
                     /translation="MKPFFRAMWPVRALRMAPLAGIVAAVLIASAVPTTAQQPNPNVR
                     PPAGAVQSSQPDAPNAPGAVRPWNYDAEMWHQLRQGIEGKVSIQDGKAGRLVDSSGEA
                     WRNFRNGPLPTYGAWFLAATLGVIALFFLIRGRIKIEHGWAGRTIERFNSLERMGHWL
                     LATSFILLALTGLNILYGRYWLLPVIGKEAFAAITLAGKLVHNYVSFAFMFGLLWVFV
                     AWVRHNIPHWRDLQWLAMGGGMLVKGVHPPSWKFNAGQKILFWLIILGGISISLSGLS
                     LMFPYELPMFAKTFSAINSIFGTDLPTALSVNQEMQYSSLWHAMMALFLTAVILGHIY
                     IGTLGMQGAFSAMGSGQVDIEWAKQHHSLWAEEVLKGEGERPVDTGEVRPQPAE"
     gene            complement(11845..12435)
                     /gene="fdh3B"
                     /locus_tag="VF226_18460"
     CDS             complement(11845..12435)
                     /gene="fdh3B"
                     /locus_tag="VF226_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HEX7076025.1"
                     /translation="MARMKFLCDAERCVECNACVTACKNEHEVPWGINRRRVVTINDG
                     KPGERSISVACMHCSDAPCAAVCPVDCFYQTEEGVVLHNKDLCIGCGYCFYACPFGAP
                     QYPQASNFGSRGKMDKCTFCAGGPETNQDVMFKKYGRNRLAEGKLPLCAEMCSTKALL
                     AGDGDVVSDIYRQRVVARGFGSGAWGWGKAYPGTPG"
     gene            complement(12439..15381)
                     /locus_tag="VF226_18465"
     CDS             complement(12439..15381)
                     /locus_tag="VF226_18465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX7076026.1"
                     /translation="MLRKKANGAAGRARLSSTLHVESHGTVDRRAFLRNSGLAIGGLA
                     AASTLVTGRVQKAEAQTSDASGNNVEIRKTVCTHCSVGCTILAEVQNGVWIGQEPGFD
                     SPLNLGSHCAKGASAREHAHGERRLKYPMKLVAGKWQRISWDQAINEIGDKMLQIRET
                     SGPDSVYFLGSAKFSNEQAYLFRKFYAFWGSNNGDHQARICHSTTVAGVAQTWGYGAM
                     TNSYNDIQNSKAIFVIGSNAAEAHPVAMQFLLRSKEQNNAQLIVCDPRFTRTAAHANE
                     YVRFRPGTDIPLIWGILYHIFENGWEDKEFIRQRVWGMEWVREEVKKWTPEEVENVTG
                     VPGSQLRRVARTLASNKPGTLVWCMGSTQHTVANANVRAYCILQLALGNMGVAGGGAN
                     IFRGHDNVQGATDFGVEPGTLPGYYGLSEGSWKHWARVWDVPHDYLVSRFASKKLMET
                     AGIPLSRWVDGVLEDKANVDQPDLLKAVFFWGHAPNSQTRGPDMKKAMEKLDLLVVVD
                     PYPTATAVMHDRTDNTYVLPAATQYETVGSVTASNRSLQWREKVFEPFFEAKTDHEIM
                     YLFAKKLGFDKEMFKHIKVENNEPLVEDITREFNRGMWTIGYTGQSPERLKLHMANQH
                     TFDRVTLRANGGPCDGDYYGLPWPCWGNPDMELPSGKKGHPGTPNLYDTSKHVQDGGL
                     NFRALWGATVKGQSDKYTRNDENLDNLLAVDSYPVGNELKSGHPAVSLGLLKKLGWDG
                     DLTPEELATIQRVGGDKADAVTFTTDLSGGLQRVAIKHGLAPFGNGKARAVVWNFPDP
                     VPLHREPLYTPRRDLVAKYPTHTDRKMNRLPVLFESIQKNDFSKEYPIVLTTGRLVEY
                     EGGGEESRSNPWLAELQQNMFVEIHPTDANNLGIKDGQMVWVEGPEKGKVLVMAMVTE
                     RVGKGVAFMPFHFAGHFQGKDLRSKYPPGCDPYVLGEAANTATTYGYDVVTQMQETKT
                     TLCKIWRA"
     gene            complement(15452..15643)
                     /locus_tag="VF226_18470"
     CDS             complement(15452..15643)
                     /locus_tag="VF226_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX7076027.1"
                     /translation="MKRQDDKAVTDRRSFLKLAGAGMAASGAAVAAGSPASAKESKPR
                     EGLYRETAHVKRYYELARF"
     gene            complement(15680..16276)
                     /locus_tag="VF226_18475"
     CDS             complement(15680..16276)
                     /locus_tag="VF226_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEX7076028.1"
                     /translation="MDEAVSAVAAEDLLRAQLYRLLARFLSAPPSQSDLEAAAALQGD
                     DSDLGRAISTFAHIAGRSSATDVADEYQVLFIGLGRGELVPFGSYYLTGFLNEKPLAK
                     LRQDMARLGIARDPDVRDPEDHIASLAEMMAGLIDGTFGKPLPLDEQKRFFDTHIGCW
                     ASYFFRDLQQAKSSVLYSALGSVGSIFLEIEESAFKLD"
     gene            16773..17798
                     /gene="moaA"
                     /locus_tag="VF226_18480"
     CDS             16773..17798
                     /gene="moaA"
                     /locus_tag="VF226_18480"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7076029.1"
                     /translation="MLGQNARTADAGMVDPFGRRVTYLRVSVTDRCDFRCVYCMSEHM
                     TFLPKKDLLTLEELDRLCSAFVAKGVTKLRITGGEPLVRKNIMWLFRALSRHLETGAL
                     EELTLTTNGSQLPRYATELADCGVKRINVSLDTLRPDRFKAITRWGDFSRVMEGLEAA
                     DKAGLAVKINAVALKGVNEDEIEDLLRFAHGRGYDLTLIETMPLGDIDGDRTDQYLPL
                     SIVRARLMDRFTLEDIPYRTGGPARYVRVAETGGRLGFITPLTHNFCESCNRVRLTCT
                     GTLYMCLGQEDAADLRTPLRASPDDALLSAAIDEAISRKPKGHDFVIDRRTRRPAVSR
                     HMSVTGG"
     gene            complement(17812..18114)
                     /locus_tag="VF226_18485"
     CDS             complement(17812..18114)
                     /locus_tag="VF226_18485"
                     /inference="COORDINATES: protein motif:HMM:NF022786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HEX7076030.1"
                     /translation="MKEPNRQERRAMRAAGHLDTMAFLKVADKFIDLANRENTRVKAT
                     ELHMAFLYAAARYNAYVAKVVLDVDNHEEFVKKMTGEYAEMLRQHLGDPSLEPPEK"
     gene            complement(18128..18826)
                     /locus_tag="VF226_18490"
     CDS             complement(18128..18826)
                     /locus_tag="VF226_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076031.1"
                     /translation="MMLVRRCLIALVILYGALLYGAPDVWAREKDPRLPPGTDPGGVA
                     LALISTGIDYTLPEIAGRLARDGEGQLIGRDFVDDDPLPYASAEGDQGGRGAVIARAI
                     LAEAPSTRLIPVRIDPQKPGSVPEALAFVARTPARAALVDPVGSDGEGDLMRKAAQEA
                     RHLLIVIPAAGALADLENVLAVASRNGGTSSEPRPDTDVAIQSMRLAAEIAESDPSLD
                     GAGLKRAVLARLAR"
     gene            complement(18823..19404)
                     /gene="mobB"
                     /locus_tag="VF226_18495"
     CDS             complement(18823..19404)
                     /gene="mobB"
                     /locus_tag="VF226_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX7076032.1"
                     /translation="MRSTTKPQRTPVIGIAGWKKSGKTTLTVRLVEEFTRRGLKVATV
                     KHAHHAFQIDDGETDSARHRRAGAKQVAIVSSRRWAIVNELDGAPEPSFDEVISWLEP
                     CDLVVVEGYKSAPIPKIEARRLQSLTKRPLAPEDPMIVAIASDHAVEGATVPVFSLDD
                     IASIADFIDRTIGPLGKRESGLAAHSAATPSEE"
     gene            complement(19401..20045)
                     /gene="mobA"
                     /locus_tag="VF226_18500"
     CDS             complement(19401..20045)
                     /gene="mobA"
                     /locus_tag="VF226_18500"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX7076033.1"
                     /translation="MRGDPVELVTGVLLAGGLSRRMGGGDKCLRDLAGKPMLAHVIER
                     LRPQVGAMVINANGDPERFAAFGLDVVPDTVGGFAGPLAGVLAGMRWSATRAPEAQWI
                     VTVSTDAPFLPLDLTRRFLEAIGDAPRTIAMAASGEDVHPVIGLWPVALADDLEEQLN
                     AGVRKVLHWTGRHASVVVNFPFLCFNGRKVDPFFNANTPEELDEARDLIESRSP"
     gene            complement(20050..>20838)
                     /gene="fdhD"
                     /locus_tag="VF226_18505"
     CDS             complement(20050..>20838)
                     /gene="fdhD"
                     /locus_tag="VF226_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX7076034.1"
                     /translation="SVVTERPLTLFLNAREIVTMMTIGDRPDLLAVGYLLNQNMLRAD
                     DEITGIEYEEDIETVVVRTRRQTDFEEKLKKKIQTSGCAQGTIFGDIMEEFDRAKLNP
                     DARIRTSWLYQLTKKINTEPSLYLKAGAIHGCVLCQEDKPLIYMEDVGRHNAVDKIAG
                     YMFLNKIKPDDKTFYTTGRLTSEMVIKTVKMGIPILMSRSGFTAWGVDLARRAGLTLI
                     GRARGKRFVALSGIERIIYDADVTRVGDEDRRHARKGSLAAAED"
BASE COUNT         3711 a         6778 c         6509 g         3840 t
ORIGIN      
        1 gtcgttccgc acgatgagct cgatgccgag gtcgcacgct ggtgcgctga gatcctcgag
       61 cattcgccga cggcgatcgc catcgccaag cgctccttca atgccgacac ggagcacatc
      121 cgaggcattg gcgccatggg aatgcaaacg ctcaaaatgt attatcaaac ggaagagtcc
      181 aaggaaggcg ttcgcgcctt cattgagaag cggaaaccgg atttccgcaa atatgtaaaa
      241 tgacgggcgg aaataaacgc accgcaaagc cggaaattct gcatgccgat agacaaagac
      301 tccttcgacg agttcctcgg cacgatccga cggttcgtgc gcgaacggct catcccgaac
      361 gaggcccgcg tggccgagga cgacgccatc cccgacgaca tcgtcgcaga catgaaatcg
      421 ctcggcctgt tcgggctcgc cattccggag gactacggcg ggctcggcct ctcgatggag
      481 gaagaggttc tggtcatctt cgagatcgca agggcatcgc ctgccttccg gtcctacttc
      541 gcaacgaatg tcggcatcgg ctcgcaggcc atcgtcatgg acggaacgga agagcagaag
      601 cggcggtatc tgccccgact ggcgtccgga gagatcatcg gctccttcgc gctgacggag
      661 cccaactccg gctcggatgc tgcttccctg caaaccaccg cacggcgtga cggcgatttc
      721 tacgtgctca acggcacgaa acggttcatc acgaatgcgc cggaagcagg cgtgttcacc
      781 gtcatggcgc ggacggatcc ggacaagaag ggtgccgatg ggatctcggc ttttgtcgtc
      841 gatagagata cggcgggcct gaccgtcggc aagccggacc ggaaaatggg ccagcggggc
      901 gcgcacaccg ccgacgtcat cttcgagaac tgccgcgtgc ccagggatgc ggtgatcggc
      961 ggcgtcgaag gccaaggctt caagaccgcc atgaaggtcc tcgacaaggg ccggctgaat
     1021 atctctgcag cgtgcaccgg tgcggcagag cgcatcctgt cggatgccct tgcctacgct
     1081 ctcgagcgcg agcagttcgg caagcccatt gccgagttcc agctcgtgca ggccatgctt
     1141 gccgattgcc agaccgaaat cctggcggcc cgctcgatga tcctggaggc agcccgcaag
     1201 cgcgatcgcg gcggcgacgt caccatggag gcttcgtgct gcaagctctt cgcgagcgag
     1261 atggtcggtc gcgtcgctga tcgcgctgtc cagatccacg gcggcgccgg ttacatggcc
     1321 gagtacgctg tcgaacggtt ctaccgcgac gtccgccttt tccgcatcta tgaagggacg
     1381 agccagatcc agcagctcgt cgttgcccgc aatatgatcc gcaaagcgcg ggccgaagct
     1441 tagcagcgat gctgcggctt cggccagatg ttcggacgat ccggaaccgc ccttcggact
     1501 tatgccttcc tgcctcatga atgactcgca gcagcagccg gcagaacatg atcttttcgg
     1561 gcgcctgcgc tcgttaaaag gcgtgcggct cgaatggccc atccttctca tcgtgctgat
     1621 cgtcgcctcg gcgatatggg gcttcatcga gcttgccgac gaggtctctg aaggcagcac
     1681 ggacgccatc gaccgcaggc ttttgctgtt gctccgtgac cccaatgacc cgttcaagat
     1741 gcttggcccg agctggctcg gagagctgat gcgggatttc acgagcctcg gcgggactgg
     1801 cgtgctcaca ttcgtgacgc tcagcgcaac ggggtttctg gtgctcgagc gcaaacggca
     1861 cgcggccgcc ttgctcctcg tttccgtcgt cggcggcgtc atcctgagca atgtgctgaa
     1921 actcacgttt gcccgcccga gaccggagtt ttccgactac gagccgctga tcgctacggc
     1981 cagctttcca agcgcacact cgatgatgtc ggcggtggtt tacctgacga tcggcgcctt
     2041 gattgcgcgt atgcgccccg atccgctggt cagggcctat gttctgagtc tggcgatttt
     2101 cgtcgctgtg atcgtcggga tcagccggat ttatctcggt gtccactggc cgaccgacgt
     2161 gctcgcgggc tgggtcgtcg gctccgcctg ggcgctcttc tgctggctgg tcgctctgct
     2221 cttgcagagg cgcggcgatg tcgagaatcc ctgagacgtt gggctgcgac ggcggctcgg
     2281 cgactacgcc agccgctttt ccataaacaa gctcagcggg tctgcgaggt agctgccgaa
     2341 cggcgcgcac tcgttgaagc cgcgcttgcg ataaagtgcg atggcttccg gctgtctgat
     2401 cccggtctcg aggcgcaaga tcgtcagtcc cttctcccgg gccgctccct cgattgcatc
     2461 cagtattttg tggccgatgc cctgaccccg cgcttccgga tcgacccaca tccgcttcac
     2521 ctcgccggag ccatcctcgc cgatgatgag ggctccgcag ccaagcgcct tttctccctg
     2581 ccgggcaacg aagaaaacgg tgttgggcgc catcaacgcg gcgagatcca ccatgtggtt
     2641 gctttcaggc ggatagaggc tctcagcgta ggcatccgag gcagccagaa aggcgacaat
     2701 ctcggcctgg tccggacttt ccgtgcgaac ggcgacctcg gatgtctcct gcgccggctg
     2761 cggagccttc ggcgctgtac ggccgagccg gtcccactcc gcggcttcgt catccgagag
     2821 gaagccttga ccaaccttgt aggccgtcct cagaagtcct gacggaaccg cgcgggccgc
     2881 gtccgtgaaa tcatccatat aatcgctgag ctgatcgggg ccggaaagca ccgggtccga
     2941 cagccataac ttgttgagcg ccttgttccg aacctcgtcc ggcaccttgg cgctcatgaa
     3001 cggcttgtag tcggaatgag catcgagctt gtcgaaatcg atgctctcga tgtcgagggc
     3061 ggattgccgg tcctccgttt gggcttcggc accatccaca gtttcagacg ggtccgcctg
     3121 ggaatgctga acctgcccgg acgaattcgc tgcggacgca gccccttctt ccgtggccgc
     3181 gacctcggtc ggcctctgcg cgcctttctc cgcctcgcgc ttcagcctcg accagcgact
     3241 gaggaacggc tcgtccttgt cagtcatcgc caccttcgcc cctgcgcctt gcgcgcattc
     3301 tttcccgaag cacgaagatc ggctcctgcc cgaatctgtg ctcctcctcg atctcgaact
     3361 tttgccgctg ccgcttgacg aagcgctcct cgacatgatg ggcgtcgacg aactcccgga
     3421 ccagatcgat cagcagctgc ggcatcggca gcagctcgat ggtctcgaga cctgattccg
     3481 catacgcttg ggcatcgtgc ggtgaagcgg tcaccatgtg aaccgtcacc ggccaatcgg
     3541 agtcggcccc gggatcgttg cgcagcacga cgtagacggc aggctcgccg gtctcgaggt
     3601 tggtcttgta gccgggggtc tccttgcgat gcagctcgat gggcagagtg gccgcgtgat
     3661 agtagacaga cctctcgtcg cgacgcagct cccgccagcc gctgatctct ggcgggtgca
     3721 gaaaaacgtc gacagcgcgc cagacgtact cctgccaagg gtggtctatt ttctcacgag
     3781 cgactacaac cccgacagga actgaaatgg ccgaactcat gcagtgagac tctttcttcg
     3841 ctgccagccc gcgtattcaa ggtctatact cgggtttcca taagcggtcc atcatacggc
     3901 cggacaggat cacgcgacag gtgcacgcgg catgtcagga acagagagaa tgagcgggac
     3961 gaagccgaag cttctggtct gcaactgcca gcgcacgatg gatatcgacg gtgcgcggct
     4021 ggcgaagatg ctcggcctgg aggaaccgct caaagtgcat cgcgagcttt gccgggccga
     4081 gatcgcgagc ttcgagtcgg ctctcgaagc gggacccgta catgttgcct gcacgcagga
     4141 ggctccgctc ttccgcgatc tcgccgagga caaggggctc gccgagcggg cgttgcggtt
     4201 caccaacatc cgcgagcggg ccggctggag cgaggccaaa tcggctgcgc tgcccaaaat
     4261 ggccgccctg ctcgccgagt cggcgttcgt gccgaagcca gcgggtctga tcacgctgaa
     4321 atccgagggt gtgtgcctcg tctacgggcc gggacagatc gccctcgacg tcgcacagga
     4381 gctcggcagc cggctcagcg tgagcgtcct gctgatgacg gatgccgccg acgccattcc
     4441 gccgagcatc gccgacgtcc cgatccaccg cggccgcatt cggaaagcga cgggttacct
     4501 cggtgcgttc gagatcgagg tggacggcta tgcgcccatg ctgccgtcct cacgctcgac
     4561 gctcgaattc gtgatggcgc gcgacggcgc gaaatcccag tgcgacatca ttctcgactt
     4621 gagcgggggc cagccgctct tctcggacgg cgggcggcgc gacggatacg tgcgggccga
     4681 cccgtccaat ccggcggccg tcgccaaagc gatgttcacc gtttcggacc tggtcggcga
     4741 gttcgagaag ccgcgctacg ttgaggtgga caccgccatc tgcgcgcact cccggagcca
     4801 gaagatcggg tgcaccaact gcctggattc ctgccaaacg ggcgcgattt cgccggctgg
     4861 cgaccacgtc gtcatcgatg cgggcgtttg cgccgggtgc ggcacgtgca gtgcggtgtg
     4921 cccgaccgga gcggtcagct acaagttccc gacccgagag gatctcatca cgcgcgcaca
     4981 agtgctgctc aaggcttacc gcacggccgg gggcacgcga cccatcctgc tgctgcatga
     5041 cgaaaagcac ggcagcccga tcatttctgc catcgcacgg ttcggccgcg gtctgccggc
     5101 aaacgtgatt ccgctgagtc tctattcggt gttccagctc ggccacgacg tgcttgcgtc
     5161 catgctggcc tcgggagccg agcagctcgt cattctggcc tccggccagg ctccgggaga
     5221 gctgccgccg ctcgaaagcc aggtcgagct actgggcgcc ttcctcgagg cgctgggcca
     5281 tcaggggccg cgggcgcgcc tcgtcgtcga gcaggatccc gatgcggtcg aaagcctgct
     5341 ctatgatctg ccggctttgg ttgagctcac gccgcgcttc ttctccaccg cgggcagcaa
     5401 gcgcgaggtg gcccgcacgg ccctcgccac tctgaacgaa ttggcgcccc agccagcgac
     5461 cgttatcccg ttgccgaaag gagcgccgta cggccgcgtc cacatcgacg tcgagggctg
     5521 cacgctctgc ctgtcgtgcg tcagcgcctg ccccgccaat gcgctgtccg accatccgga
     5581 tcgcccacag ctcagcttca cggaggcggc gtgcgtgcag tgcggtgtgt gcgttgcgac
     5641 ctgccccgaa aacgtcatcc ggctggagcc gcgctataac ttcacgccat ccgcgatgac
     5701 gcccgaggtc ctcaagggcg aagagccgtt cgagtgcatc agctgcggca aaccgttcgg
     5761 cgcccgggcg acgatcgaga aagtcatcga gcggctcaag ggccactcga tgttccgcga
     5821 cgaagagcag cttcgcgtga tccagatgtg cgacacctgc agggtggtcg ccgtcgccga
     5881 gtcgagcgac gacccgttca agttcggcga acgcccccgc atgcgcacca ccgatgacga
     5941 catcgccgaa gccgaacggg cgcgagcaat gcgcaagccg gaagactttt tgagctgaac
     6001 ggaatgcggc gatggccgtt cggggattgc cgcattttcc cgataggaac gacgcgagac
     6061 ctgctgctcg agcaggcgag gctcaggcag ccatgcgcag cggcatgttc accagcaggt
     6121 tctgcggctt catcttcagt cggttggccg gtgtcatggc aaggccgaga tagatcggac
     6181 ggcctttcac acggtcgatg acggtgcggt catcgcgaac gtgcatgcga aagagagcga
     6241 cgatccgggc cagatcatcg gcgccgaccg gcttgccctc gtagagcgcg tttaggatct
     6301 gtgtcgcttc gcggtcgagc ccgatgtgcc agcgccagtc gctgtcctcg acctttgggc
     6361 gcggctcgac gcgcacttca agtcgcatca tgtgttggag ccaggcctcc aggacccgcg
     6421 caaaagcatc cagcgccggc tgctcgaagc ggaagtcgat caccgtatcg aagcggtccg
     6481 atcgctccca gtagatctct ttgttatcct cgctcaggac gtcgagctcg accgtacgca
     6541 tcggcgtgcc ggtctcggcg agaagctgcg ccaggccggt ctcgcccgag ctcccgtgca
     6601 tttcgacgat ctcctcgtca gccagcagca gccggccgcc ttcggtcgac acgatctgct
     6661 cccgaaagaa cagctcggca gccctgagcc gcatgggatc gcgcacgtct ttcaggatgt
     6721 tgcgcagaat caggtgcacc atctggtcga cgaagacagg gggtaccgtc gtgttggtgc
     6781 cgcgcgcgat ccggagatag gcgccctcga tcgtgccggc cgacatcagc agctcgcgaa
     6841 atgcaaggac aagccgataa ttgtcggcgg tatcagggtc ggccagacgc gccagccgcg
     6901 cctcgtcgac cggcatgagc ggatcggcga gcagttcctc gtgcagcctg atttcctcgt
     6961 cacaggaggt ctcgatcggg tgaacctccg gccgcgtcca gtaggccagc aggagttgcg
     7021 gcgtaacctt cagccagccc tccgcgccta tttcgacgag atggaaaccc gcagacttcc
     7081 agaagtcgcg catcaatcgg gctccaccgt ccagatccgc gcgtgcatgg gctcaccggt
     7141 cggcgctttg atcgtgcgaa attcctcctt gatctggcct tccgggtcga aggtgcggcg
     7201 gaccgtgaaa accgtattga tcggagcgac ccggcaaaga tcgagaatga aggccagctc
     7261 ttcctgcgcg gctggccaag cggcatcgat atcaggggcg ccgtaggcct cgacgaagtg
     7321 gcaagcgagg cggaagatga tctcgtcggc ggcggcctga tccgcctccg ccactgtcgc
     7381 aaacgtgctc cgcccgaagc tttccacgcc caagaaaccg ttggcgaaag cctgcttcac
     7441 ctttcccgtg acatcggcgg gggcgaggtg cgcgaacgcg aatgctcccg aaacggccca
     7501 ttcgtccgaa gccgcggcat gctcgaatac gcgatcatcg gagctgtcga agcggatcgt
     7561 ccgcaggagt ttcaccgcgc gagctcccgc ccataaactt cgatatgctc gatcagcggc
     7621 agaacgcgcg ccggccctgc ctcgggccga atgagcagtc caccttcctc atcgagcccg
     7681 agcacctgca cgacctgccg cgtgccctcg tgctccacct ccatggcctt gccgtccgcc
     7741 tccgcgcgag ccatccagct atcgtggatc gggcggaagc cgtcctcctg ccatgtgttg
     7801 agccagctca gcagatagga agcgtaacac tggagcaggt ccgtgcgggt gatctcgccg
     7861 ccgccttcct cggcgagcgc tgtctggccc ggcagctcgc ccggctcttt gccgggatca
     7921 tcgtggacga ggcggaggct catgccgacg accatccaat cgggaacctg gcccgcctcg
     7981 gtcgccggca gcccgactac cacgccgcca cactctgcac cattgacgag tatcgtccca
     8041 ggccagcggt gccagacgca aacagcaggc ggcagcagag cacccaggct gtcggcgagc
     8101 gcgacctgcg cgagggcgtt catctgcagc gccgtcccga gcggtacctc aggctcgagg
     8161 acgatggcgc attcggcccg gctcgtgttc cggctccaga cgaggtcgcc ggcgcccaac
     8221 gtaccccggg aagcctggcg acacgcgtgc gaaaacgggc gtagcggcgc cttgacgtcg
     8281 tggccggcga gcagcggcgg aaaaacaggt tccggaaggt gcatgtaagg tgcttcagcc
     8341 aacctcgatt tcgccctcga cgacgtgctt caggtcggtg ccctcggcgg tcagctccat
     8401 ccggaccttc agcggcccct gaccagaaag ctgcttctcg cgaacgatgc gctcgatctc
     8461 cctctgcgag gtcacgccga cgtgcttcag gaatttgcgc agtgaaatat tgaacctgtc
     8521 ttcaggctcc ttatcagtca tcgcgacctc ctgacggctc agagccgtac gttttgcgta
     8581 gtgtaggtgt cctgaaccga catggacatg taattcagtc ctaccgcggc caaggcggat
     8641 atcaccacga gcagcgcgag gctgcccagg aaggctctca ttgctctcct cctatcattt
     8701 cgatccatcg cccgtaccag tgccgccgtc aacagatggc ggcgccgttg cgaccttgag
     8761 atacgccacc agcgcgtccc gctgcgcttt gtccgtcatt ttctgcagtg gcatcttgga
     8821 gccgggagtg aattcatcgg ggccgagctc gaagagcttc gatacggttt cctccgtcca
     8881 gacgatgtcg agttgtttga gcgcctcgga gtagggatag cccggcagcg tcccgatgcg
     8941 gcgaccgaac aggttgtaga gcgtcggccc ggcgcggttc ccgccatccg gcgtcaacgt
     9001 atggcatatg ctgcacttcc gggcgaactg gattcgcccc tgctccagaa cgtcatcgct
     9061 ttcaccgacc tgctgaaagc ggcgcgggaa ttcgctttgc acgggttcga acggctgccg
     9121 cgggcggata tgccacagcg tagcgaaatc atcgagcccg ccgtagtaga ggctgcggcc
     9181 ttctctggtg aaggccagcg cccagatcgg tccgtaggtg ttctgatatt cttcgagaac
     9241 cgcaccatct cccgaccgcg agacgcggat cacgccgtcc gcgctgccgg tggcgatgag
     9301 ggatggcttg tccagcacgg cgaccgccag caccggccgc tcgtgctgct tgagctgcac
     9361 gacgaccttg ccgttgccgg gatcaatcac accggccgct ccgttgatcg cgccgaaaac
     9421 gagccgctcg ctatcgggaa gccgctcaag aacgttgatg ccccagccgt gccgatagac
     9481 tggccgctcg aagctgccgt cctcccggcg gaagacgccg atgctgccgt cgtagctcgc
     9541 agtgtaaacc cggctcccat ctgcggagaa ggcgacggca ttgaccgggc ccgcgtggcc
     9601 cctcagaacg gggccaggcg cgagctgttc caaatcccac agccgggccg tgtggtccca
     9661 actggcagat gcagcccagt ggccgtcacc cgtcaccgcc agtccgacga tcttgccctg
     9721 gtgaccgctg aagcgatgca cgagcgcacc cgtcgagatg tcccaaatgg agacggtgcc
     9781 atcgtcgccg gccgaaagca cccggctggt gccagggacg aaggccaccg cattcaccgc
     9841 accgtcgtgg tcctcgaacc gtgcgagctg ccggggctgc tcgcctgaga tgtcccacgc
     9901 catcatggcg tagtcgaatg accccgttag gacacggttt gaagcggtat cgatgcgcac
     9961 cgctttgatc gggccgccgt gtccgacgag gttgccagct gcggcgcgcc gttcgtctgc
    10021 tgacttctga ccagcagcag gcgagccgcc cggcggcagc gcccgcgcat cctgggcagc
    10081 ggcagtgcca attgccccag ccaaaatgag cagcaggatc gccaggcacc cgatcggccc
    10141 ggtcggcggg gcggcgcgct gtgggcggca agcgaaacta gccctgtccg cagcgtatcg
    10201 ccccgcccga accgcctgag ccatgacaga gcatcactcc gccggttgcg gccgtacctc
    10261 gcccgtatcc acagggcgct cgccttcacc cttgagcacc tcctcggccc aaaggctgtg
    10321 gtgctgcttg gcccattcga tatcgacctg gcccgatccc atggccgaga atgcaccctg
    10381 catacccagc gtgccgatgt agatatgacc gaggatcacc gccgtcagga agagcgccat
    10441 catcgcgtgc cacagcgagg agtattgcat ctcctgatta acgctcagag ccgtcggaag
    10501 gtcggttccg aagatgctgt tgatggcgct gaaggtcttc gcgaacatcg gcagctcata
    10561 cgggaacatc agcgacaggc cggacaggct gatcgagatg ccgcccagaa taatgagcca
    10621 gaacaggatc ttctggccgg cattgaactt ccagctcggc gggtgcacgc ccttgaccag
    10681 catgccgccg cccatcgcga gccactgcag atcgcgccag tgtgggatgt tgtgccgaac
    10741 ccaggcgacg aacacccaaa gcagcccgaa catgaaggcg aacgacacgt agttgtgcac
    10801 caacttgccg gccagggtga tggcggcaaa ggcctccttg ccgatgaccg gcagcagcca
    10861 atagcgtccg taaaggatat tgaggccggt cagggcgagc aggatgaacg aggtggccag
    10921 cagccaatgg cccatacgct cgaggctgtt gaaccgctcg atggttcggc cagcccagcc
    10981 gtgctcgatc ttgatgcggc cgcggatcag gaagaacagc gcgatcacac cgagggtggc
    11041 agccagaaac cacgcaccgt aggtcggcag cgggccgttg cggaagttgc gccaggcctc
    11101 accggaggag tcgaccagcc ggccggcctt gccgtcctgg atcgagacct tgccctcgat
    11161 accctgacgc aactgatgcc acatttcggc atcgtagttc cagggccgaa ccgcaccggg
    11221 tgcattgggc gcatcgggct gcgaggattg tactgcgccc gccggcgggc ggacgttggg
    11281 gttgggttgc tgcgccgttg tcggcacggc cgaggcaatg agcactgcgg ccacaatgcc
    11341 ggcgagcggc gccatacgca gcgcacgcac tggccacatt gcacggaaga acggtttcat
    11401 ctcgtgtcct ctcggttccc tcgaggcgcc tgtcccgcag ggcgagtcta gcgcatgatc
    11461 ctgctgcggt cttgtaaagc ccggagttgg tcgtcactca atggctgaag cttctcaccc
    11521 tggtacacgc cgggttcgaa tgacaacggc cggccctgct cctgctcgcg gcagccggcc
    11581 agcacaagca ccgcccccaa gaccagagtt gttattatcc ctcggtgcat ctcatttcct
    11641 ttcgacaggc tggcgcaacc ggtcgccgcc cgtcagggct catgaaggcc ttctcataga
    11701 aaggccttct catagctcaa gcctcgccca gaaggggtgc acaggcgaat tcaagcacat
    11761 ttgcaaggcc aaagacgggg cggtcgatga ccgccccgtt gcaatgtcgt ccgtatcaac
    11821 gaaccggaga caacacccct cagatcagcc cggcgtgcca gggtaggcct tgccccagcc
    11881 ccaggcgccc gaaccgaaac cgcgcgccac aacgcgctgg cgatagatgt ccgacaccac
    11941 atcgccgtcg ccggcgagca gcgccttggt cgagcacatc tcggcgcaaa gcggcagctt
    12001 gccttcggcg agccggttac ggccgtactt cttgaacatg acgtcctggt tcgtctccgg
    12061 gccgccggcg cagaaggtgc acttgtccat cttgccgcgc gagccgaagt tggacgcctg
    12121 cgggtactgc ggcgcgccga acgggcacgc gtagaagcag tagccgcagc cgatgcacag
    12181 gtccttgttg tgcaggacca cgccttcctc ggtctgatag aagcagtcca ccgggcagac
    12241 cgccgcgcac ggcgcgtccg agcagtgcat gcaggcgacc gagatcgagc gctcacccgg
    12301 cttgccgtcg ttgatcgtga cgacgcgccg acgattgatg ccccacggca cctcgtgctc
    12361 gttcttgcag gcggtgacgc aggcgttgca ctccacgcag cgctctgcgt cgcagaggaa
    12421 cttcatgcgt gccatggttc aggccctcca gatcttgcag agcgtcgtct tcgtctcctg
    12481 catctgcgtg acgacgtcgt agccataggt cgttgcggtg ttggcggcct cgccgaggac
    12541 gtaagggtca catcccggcg ggtacttcga gcgcagatcc ttgccctgga agtgaccggc
    12601 gaagtggaac ggcatgaagg cgacgccctt gccgacccgc tccgtgacca tcgccatcac
    12661 gaggaccttg cccttttcag ggccctccac ccagaccatc tggccgtcct tgatgccgag
    12721 attgttggcg tccgtcgggt ggatctcgac gaacatgttc tgctgtagct cggcaagcca
    12781 cgggttcgac cggctctcct cgccgccgcc ctcgtactcg acgaggcgac ccgtcgtcag
    12841 aacgatcgga tactctttcg agaaatcgtt cttctggatc gactcgaaca gcaccggcaa
    12901 gcggttcatc ttgcgatcgg tgtgggtcgg atacttcgcc acaagatcgc gccgcggcgt
    12961 gtagagcggc tcgcggtgga gcggcaccgg gtcggggaag ttccagacca ctgcacgcgc
    13021 cttgccattg ccgaacggtg cgaggccatg cttgatcgcc acgcgctgca aaccgcccga
    13081 caggtccgtg gtgaacgtca ccgcgtcggc cttgtcgccg ccgacccgct ggatcgtcgc
    13141 cagctcctcc ggagtcaagt ctccatccca gccgagcttc ttgagaagcc cgagcgaaac
    13201 cgcgggatgg ccacttttga gctcgttgcc gaccgggtag ctgtcgacgg cgagcaggtt
    13261 gtcgaggttc tcgtcgttac gcgtgtactt gtccgactgg cccttgaccg tagcacccca
    13321 cagcgcacgg aagttcaggc cgccatcctg cacgtgcttg gacgtgtcgt acaggttggg
    13381 cgtgccgggg tggcccttct tgccgctcgg cagctccatg tcgggattgc cccagcacgg
    13441 ccacggcaga ccgtagtagt cgccgtcgca cggaccgccg ttggccctga gagtcacgcg
    13501 gtcgaaggtg tgctggttgg ccatgtgaag cttcagccgc tccggcgact ggccggtata
    13561 gccgatggtc cacatgcccc tgttgaactc acgggtgatg tcctcgacca acggctcgtt
    13621 gttctcgacc ttgatgtgct tgaacatctc cttgtcgaaa ccgagcttct tggcgaacag
    13681 gtacatgatc tcgtgatcag tcttcgcctc gaagaacggc tcgaacacct tctcgcgcca
    13741 ctgcagcgag cggttcgatg cggtcactga gccaacggtc tcgtactggg tcgccgccgg
    13801 cagcacgtag gtgttgtcgg tgcgatcgtg catgacggcc gtagccgtcg ggtaaggatc
    13861 gaccacgacc agcaggtcga gcttctccat cgccttcttc atgtccggtc cgcgcgtctg
    13921 cgagttcggc gcgtggcccc agaagaagac ggctttcaga aggtcgggct gatcgacgtt
    13981 cgccttatcc tcgagcacgc cgtcaaccca gcgcgacagc ggaataccag ccgtctccat
    14041 cagcttcttg ctggcgaagc ggctcaccag gtagtcgtgc ggaacgtccc acacgcgcgc
    14101 ccagtgcttc caggagccct ctgacaggcc gtagtagccc ggcagcgtgc ccggctcgac
    14161 gccaaagtcg gtcgcgccct gcacgttgtc gtggccacgg aaaatgttgg cgccaccgcc
    14221 ggccacaccc atgttgccga gcgcgagctg caggatgcag taggcacgca cgttggcgtt
    14281 cgcgacggtg tgctgggtcg agcccatgca ccagacgagc gtaccaggct tgttggaggc
    14341 cagcgtgcgg gcgacacggc ggagctgcga gcccggaacg ccggtgacgt tctcgacctc
    14401 ctcaggcgtc cacttcttca cctcctcgcg cacccactcc atgccccaga cgcgctggcg
    14461 gatgaactcc ttgtcctccc agccgttctc gaagatgtgg tagagaatgc cccagatcag
    14521 cgggatgtcc gtgcccggac ggaagcggac gtactcgttc gcgtgggcag cggtgcgcgt
    14581 gaagcgcgga tcacagacga taagctgcgc gttgttctgc tccttggagc gaagcaggaa
    14641 ctgcatcgcg accggatgcg cctcggccgc gttgctgccg atgacgaaga tcgccttgga
    14701 gttctggata tcgttgtagg agttcgtcat cgcgccgtag ccccaggtct gggcgacgcc
    14761 ggcgacggtg gtcgagtgac agatacgggc ctggtgatct ccgttgttgg agccccagaa
    14821 cgcgtagaac ttgcggaaca ggtaggcctg ctcgttcgag aacttggcag agccgaggaa
    14881 gtacaccgaa tcgggacccg aggtctcgcg gatctgcaac atcttgtcgc cgatctcgtt
    14941 gatggcctga tcccaggaaa tgcgctgcca cttaccggca acgagcttca tcgggtactt
    15001 gagacgccgc tcgccatgcg cgtgctcgcg cgcggaagcg cccttcgcac agtgagaacc
    15061 gagattgagc gggctgtcga acccgggctc ctgcccgatc cacacgccgt tttgcacctc
    15121 ggcgagaatc gtgcagccca cggaacagtg cgtacagacc gtcttcctga tctcgacatt
    15181 gttgccgctc gcatccgacg tctgagcctc ggccttctga acacgaccgg tgacgagcgt
    15241 cgatgccgct gccagaccgc cgatggccag ccccgaattg cgcaggaatg cgcgccggtc
    15301 gaccgtcccg tgcgattcga cgtggagggt cgacgacagg cgggcccgcc ctgcggctcc
    15361 gttcgccttc ttcctcagca taatcttctc cctttaaacg tcatgcggtc agcccggcct
    15421 tcgccacgtg ctgctgccgc aggggtggaa gtcagaacct agcgagctcg tagtagcgct
    15481 tgacatgagc cgtctcgcgg taaagccctt cgcgaggctt gctttccttt gccgatgccg
    15541 gactaccggc tgcaaccgcc gcaccgctcg cagccattcc agctcctgcg agcttcagga
    15601 agctgcgacg gtcggtgacc gccttgtcat cctgtctttt catttccagc tcctttccta
    15661 acggcccaca aacaccggct cagtcgagct tgaatgcact ttcctcgatc tcgaggaaga
    15721 tgcttccgac tgaacccagc gctgaataca ggacagacga cttggcctgc tgcagatcac
    15781 ggaagaaata agacgcccag cagccgatgt gcgtatcgaa gaagcgcttc tgctcgtcga
    15841 gcggcaatgg cttgccgaag gtgccgtcta ttaagcccgc catcatctcg gccagtgaag
    15901 cgatgtgatc ctccggatcg cgaacatccg gatcccgcgc gatcccgagt cgcgccatgt
    15961 cctgtcgcaa tttggccaga ggcttctcgt tgaggaaacc cgtcaaatag tatgagccga
    16021 agggtacgag ctcgccgcgg cccagcccga tgaacagaac ctgatactcg tcggccacgt
    16081 ccgtcgcgct gcttcgcccg gcgatgtggg cgaaggtcga aatcgcgcga ccgaggtcac
    16141 tgtcatcgcc ctgcagggcg gcggcggcct cgagatcgga ctggctgggg ggagccgaca
    16201 gaaagcgcgc gagcaagcga tagagttgtg cacgaagcaa atcttcggct gcaaccgccg
    16261 acacggcttc atccattggc gccgcactcg gcattcccaa agcccccttg aagcaattcc
    16321 gtttcccctg tcttggcggg cacgcagcga gcgacctgcc acgacagcgt ccttatgatg
    16381 catctgccgc acaattttgc aatatgggaa agggcacatt cctgaaagaa caccaaaatg
    16441 tgctcagaaa ttccatagag attgtatggg tttaaaattc gcgcatcaga acatcatgga
    16501 tcagacattc aaaattcagc agttacagag gccaaaggcg acaccatttg cttttcgtca
    16561 attttgagcg ccgtccgatt tgtgcagtgc gcaatcgcga tcgtatatcg gcaatactgc
    16621 ccctggtcga gacaccactt gatccacggc ggtgctgcgc tagtgtggcg gcgagacatt
    16681 cgtgctggaa cttcggaatc cggatactag attgggctgc aaggggcctg ctccaggcca
    16741 ttgcgccccc acgtgctgac aaggttcacc tgatgcttgg gcaaaacgca cggacggctg
    16801 acgccggtat ggtcgatcca ttcggccgtc gcgtgaccta tctgcgggtt tcggtgacgg
    16861 accgctgcga tttccgctgc gtctactgca tgtccgagca catgaccttc ctgcccaaga
    16921 aggatctact gacgctcgaa gagctcgacc ggctgtgctc ggcgttcgtg gcaaagggcg
    16981 tcaccaagct gcgaatcacc ggcggggagc cgctggtgcg caagaatatc atgtggctgt
    17041 tccgtgccct ctcgcggcat ctggagacgg gcgcgctgga agagctgacg ctcacgacca
    17101 atggcagcca gctcccgagg tacgcgaccg agctcgccga ttgcggtgtc aagcggatca
    17161 acgtctcgct ggacacgctc aggcccgacc gcttcaaggc gatcacccgc tggggtgatt
    17221 tctcccgggt gatggaaggc ctcgaggccg cggacaaggc cgggttggcc gtcaagatca
    17281 acgccgtggc cctcaagggc gtcaacgagg atgagatcga ggacctgctg cggttcgccc
    17341 atggccgggg ttatgacctg acgctcatcg agaccatgcc gctcggcgat atcgacggcg
    17401 accggacgga tcagtacctg ccgctgtcca tcgtgcgcgc gcggctcatg gaccgcttca
    17461 cgctggagga catcccatac cggacggggg gaccggcacg ttacgtgcgg gtagccgaga
    17521 ccggcggacg cctcggcttc atcacgccgc tgacccacaa cttctgcgaa agctgcaatc
    17581 gcgtgcggct cacctgcacc ggcacactct atatgtgtct tggccaggag gacgccgccg
    17641 atctgcgaac acccctgcgc gccagccccg atgatgccct gctgtcagcc gcgatcgacg
    17701 aggcgatcag ccggaagccg aaggggcatg acttcgtcat cgaccggcga acgcgccggc
    17761 cggcggtctc acggcacatg agcgtgaccg gcggatagcg gcggcggcgc gttatttctc
    17821 aggcggttcg aggctcggat cgccaaggtg ctggcggagc atttccgcgt actcgcccgt
    17881 catcttcttc acgaactcct cgtgattatc gacatcgagg acgaccttgg cgacgtaggc
    17941 gttgtagcgc gctgccgcgt aaaggaaggc catgtgcagc tcggtggcct tcacgcgggt
    18001 gttttcgcgg ttggcgagat cgatgaactt gtcggccact ttcaggaaag ccatggtgtc
    18061 cagatgacct gcggcacgca tggcccggcg ctcctgccgg ttgggttctt tcattgggct
    18121 ctccacacta tctggcgagc cgcgcgagca cggcgcgctt caggccggcg ccgtcgagcg
    18181 acggatcact ttcggcgatc tcggcggcaa gtctcatgga ttgtatcgca acgtcggtat
    18241 ccggccgagg ctccgaactg gtgccgccat ttcggcttgc gacggccagc acgttttcaa
    18301 gatcagcgag cgcacctgcc gcgggtatga cgatcagcag atggcgtgcc tcctgcgcag
    18361 ctttgcgcat gaggtcgccc tcgccgtcgg acccgaccgg atccacgagg gcagcacggg
    18421 ccggcgtcct tgcgacgaat gcaagcgcct ccggcaccga gccgggcttc tgcggatcga
    18481 tccgcacggg aatgagccgc gtggacggcg cttcggccag gattgccctt gcaataacgg
    18541 ctcccctccc gccctggtcg ccttcggccg acgcgtatgg cagggggtcg tcatcgacaa
    18601 aatcacggcc aatgagctgg ccttcgccgt cgcgggcaag gcgacccgcg atctcaggaa
    18661 gcgtgtagtc gatcccggtg gaaatgagcg ccaatgcgac cccgccgggg tccgtcccgg
    18721 gcggaaggcg cgggtccttt tcgcgggccc acacatcggg agcgccgtag agaagagcgc
    18781 cgtagagaat cacgagtgcg atcaggcagc gccggacgag catcactcct cgctcggtgt
    18841 cgcggcggaa tgagcagcca acccgctctc ccgtttgccg agcgggccaa tcgtgcgatc
    18901 gatgaaatcc gctatgcttg cgatgtcgtc cagggaaaac accggcaccg ttgcgccctc
    18961 gaccgcgtga tccgacgcga tcgcgacgat catcgggtcc tctggcgcca gcgggcgttt
    19021 ggtcagcgac tgcaggcggc gcgcttcgat cttcgggatc ggcgctgact tgtagccctc
    19081 gacgacgacc aggtcacacg gctcaagcca ggagatcacc tcatcgaagc tcggctccgg
    19141 cgcaccgtcg agctcgttga cgatggccca gcgccggctg gacacaatgg ccacctgctt
    19201 ggcccccgcg cggcgatggc gtgcgctgtc cgtctcgccg tcatcgatct ggaacgcatg
    19261 gtgtgcgtgc ttgacagtcg ccactttgag accgcgccgg gtaaattcct cgacgaggcg
    19321 cacggtcagc gtcgtcttgc cggacttttt ccagcccgca attccgatga cgggggttct
    19381 ctgtggcttc gtcgtcgatc tcaagggctt ctgctctcaa tcagatcacg cgcttcgtcc
    19441 agctcttcgg gtgtgttcgc gttgaagaac gggtcgacct tgcgcccgtt gaagcacagg
    19501 aacgggaaat tgacgacaac gctggcgtgc cggcccgtcc agtgcagcac cttgcgcacg
    19561 ccggcgttga gttgctcctc cagatcatcg gccagcgcga cgggccacaa gccgatgacg
    19621 gggtgaacat cctcccccga agccgccatc gcgatcgtcc gcggcgcgtc cccgatcgct
    19681 tcaagaaagc gccgcgtgag gtcgagcggc agaaacggcg cgtccgttga gacggtgacg
    19741 atccattgtg cctcgggagc acgggttgcc gaccaccgca tcccggccaa tacgccagcg
    19801 agcggtccgg caaatccgcc gaccgtgtcc ggcacgacat cgagcccgaa ggcggcaaac
    19861 cgctctggat cgccgttggc gttgatgacc atggcgccca cctgtggccg gagccgttcg
    19921 atcacgtgcg ccagcatcgg cttgccggcc aggtcgcgca ggcacttgtc gccgccgccc
    19981 atacggcgcg acaggccacc cgccagcaaa acccctgtca ccagctccac cgggtctcct
    20041 ctcattccat caatcctcgg cggcggcgag gctgcccttg cgggcgtgcc tgcggtcctc
    20101 gtccccgacc cgcgtgacat ccgcatcgta gatgatgcgc tcgatgccgg acagggccac
    20161 aaaccgcttg ccgcgtgcgc ggccgataag cgtcaggccc gcccggcgcg caaggtcgac
    20221 tccccaggcc gtgaagcccg atcgggacat caggatgggg atgcccatct tgaccgtctt
    20281 gatgaccatt tcggatgtca gacggccggt cgtgtagaag gttttgtcgt ccggcttgat
    20341 cttgttgagg aacatgtagc cggcgatctt gtcgacggcg ttgtggcggc cgacgtcctc
    20401 catatagatg agcggcttgt cctcctggca gagcacgcag ccgtgaatgg cgcctgcctt
    20461 gaggtaaagg ctcggctcgg tgttgatctt cttggtgagc tgatagagcc agctcgtcct
    20521 gatccgggca tcggggttga gctttgcccg gtcgaactct tccatgatgt cgccgaagat
    20581 ggtcccctgg gcgcagccgg acgtctggat cttcttcttg agcttttcct cgaagtcggt
    20641 ctggcggcgg gtccttacga ccacagtctc gatgtcttcc tcgtactcga tgccggtgat
    20701 ctcgtcgtcg gctcgcagca tgttctggtt gaggagatag ccgacggcga gcaggtccgg
    20761 ccggtcgccg atcgtcatca tggtcacgat ctcgcgcgcg ttgaggaaca acgtcagggg
    20821 ccgctccgtc acgacgga
//
LOCUS       DASVDU010000277        16400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_455082, whole genome shotgun sequence.
ACCESSION   DASVDU010000277 DASVDU010000000
VERSION     DASVDU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455082"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /gene="acnA"
                     /locus_tag="VF226_18510"
     CDS             complement(<1..1681)
                     /gene="acnA"
                     /locus_tag="VF226_18510"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX7076035.1"
                     /translation="MDSFGTASELRVGDKTFRFFSLRKLGQHGFDVDRLPFSLRILLE
                     NLLRNEDGTTVNKADIEALAGWKPYADGQKEIAFTPARVLMQDFTGVPAIVDLAAMRD
                     AMARLGGDPAKINPLQPLELVIDHSIQADAFGRPDAMRINTELDYKRNGERYAFLKWG
                     QQAFDNFRVGPPNAGICHQVNLEYLARVVFGAREGDVPAGTEKPMAYPDTLVGTDSHT
                     PMVNGLGVLGWGVGGIEAEAAMLGQPISMLIPDVVGFRLHGELREGATATDLVLTVTE
                     MLRKKGVVGRFVEFYGPGVRNVPVTDRATIGNMSPEYGSTCSIFPVDELTLDYLRFTG
                     RTGEHVALVEAYMKEQGLFHDLNAPEPVFSDTLELDLSTVEPSIAGPGRPQDRIPLRK
                     AKQAFHDALPSLMPPKPPAAQKQAAAKGNDGSRVGVWGEGSPEDVPLERPQEDAIKGL
                     RHGSVAIAAITSCTNTSNPSVMIAAGLLAKKAVERGLRRQPWVKTSLAPGSRVVTEYY
                     DQAGLTPFLEALGFDLVGYGCTTCIGNSGPLLPEVSAAVQRDGLVVASVLSG"
     gene            1933..2928
                     /locus_tag="VF226_18515"
     CDS             1933..2928
                     /locus_tag="VF226_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7076036.1"
                     /translation="MMKRRLGANGPLVSAIGLGCWSFAGAYGPTTEAESHATLSRAIE
                     LGVDFLDTADVYGHGTSEEVIGRFLKQNPSAKVVIATKGGIKRRPGSAERTFDNSPEY
                     LRSALEGSLRRLGRDYVDLYYVHRRDQSRPVEEVADTLAGFVKEGKIGGFGFSEISPA
                     TLRRAHAVHPVMAVQSEYSLWSRLPDLGMIEACRELGVAFVPFSPLGRGFLTGTVTRT
                     DDFGPTDFRKPNPRFLEPNFSLNRKTLEPFLALASRYDASPGQLALAWVLAQGDHLVP
                     IPGTRSVKHLEENAAAASIALSREQVAAIERALPRGFAHGDRYSDQQWIGAERYG"
     gene            complement(2944..3939)
                     /locus_tag="VF226_18520"
     CDS             complement(2944..3939)
                     /locus_tag="VF226_18520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7076037.1"
                     /translation="MSSFGGNIGGLVARLWASGKPGKRNDPIAGAIWVTIAMATFAGL
                     GTFGKFAMHEGVDPLQVIFFRNFFCLVLMLPLLYWRGPSLAVSAQFRLYGVRVGLAFL
                     SMMAWFSALSLIPLGELTAVGFLAPLFATIFAVFWLGEVVRARRWTALVIGFIGALII
                     LRPGGVGFGLGQMLALFSALTAGIVGPLLKQMTAQDDPDKIVFITNLWATPASLIPAL
                     FVWEWPALAVWPYLIGLGLCAVLGHVALLRGFASMDASLVFTFEFSRLPFAVAAGYLL
                     LGESTDLWTWVGALIIFSSAAYITRREAQLAKERTGVRIRDVSDPLSLTPVRLRL"
     gene            4121..5551
                     /locus_tag="VF226_18525"
     CDS             4121..5551
                     /locus_tag="VF226_18525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018493087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX7076038.1"
                     /translation="MSGKTSAGTRSGAIGRARSYLENGQFEADLARRVAYRTESQLLP
                     ESLPQLERYLREEMEPAFARMGFTCRIYQNPIAGQGPVLLATRIEDPSLPTVLGYGHG
                     DVIRGLDEQWTKGNGPWVTAREGDRLYGRGTADNKGQHTINMAAMQSVMDERGGRLGF
                     NAKFMIEMGEEAGSKGLKELVEAHREDFAADVLIASDGPRTRPERPSLNLGCRGTINF
                     DLVCELRDGGHHSGNWGGLLANPAIILANALATIVGPTGALLVKELKAPAMTKAVREA
                     LADVEIDGGDKGPKVDEWWGEPGHTPAERVYGSNVFNVLAIGAGMPGRPVNAIPPRAV
                     ANCQLRFVAGTDVAGVRPAVERHLRENGFDTVRVADPPAANDGSFPATRTEPDDPWVV
                     WAVNSFQRTSNIKPAVVPQMGGSICNDIFTDVLGMPTLWVPHSYGSCSQHAPDEHILM
                     SVSQSALELMAGLYWDLGEAGVPARS"
     gene            5604..7037
                     /locus_tag="VF226_18530"
     CDS             5604..7037
                     /locus_tag="VF226_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX7076039.1"
                     /translation="MTEPCDLSAVEARRLIGNKKLSPVELLESCLKRIEKVNPTLNAI
                     VAMDEKAAKDAAKAAEEAVMRGDDLGLLHGLPIGIKDLQATAGLRTTWGSLIYKDHVP
                     EKDEATVANLRDQGAVILAKTNTPEFGAGANTKNRVYGATGNPFDPNKTCAGSSGGSA
                     VALATGMLPLATGSDYGGSLRTPAAFCGVSGFRPSPGVVPGPERSSAFTPFSVNGPMA
                     RSIADLHLLLKAQIDLDKQDPFSSDDALRIPEELPQLDLGSLRVAISTDLGCAPVDKD
                     IRRIFQERVSTFRHVFAETQDRDPDLGPVHDVFEILRGVYFVGAHRERLEKHRDLLGP
                     NVIDNTERGLKWTAADIAWANVEQSKIYRRFLDFFDDADVLICPAASVTPFPHSELSV
                     TEINGEKMPTYMRWLAITYALTTAVPSAAVIPCGVDHVGMPFGIQVVGPNGSDALVLA
                     VANALERVLAENPATSRPVPDIAALTG"
     gene            complement(7058..8263)
                     /locus_tag="VF226_18535"
     CDS             complement(7058..8263)
                     /locus_tag="VF226_18535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7076040.1"
                     /translation="MSFFGIPNPRNVALLAFSQGFFMCVQSMAIATTPLAGYALLGVD
                     KTLSTFPLFLNHAGVMMTTIPASFLMARIGRRGGFSVGAMCGVTSGLISLAAIFLQSF
                     VLLCVGSLFQGMAAAFAWYFRFAAADATEPELRPKAISLVMLGGVIAGFLGPQTAKWS
                     VHWLDPFVFGGVYVMVAVYSAFVLVLVQGLRIPGLTAAEKAQGGRPMRQIVRQPAYIV
                     ALTSSMFGYGVMTLVMSATPLAMLACGFEFQDSATVIQAHVIAMFLPSFFTGHLIARF
                     GVLPIIATGALIQMACAIINLAGIAFWNFFLANALVGLGWNFTFVGGTSLLTTTYTPA
                     ERAKVQASHDFAVYGTTATAAGLSGVLQANVGWTVVNLAALPMMGVVLLGALWLSRTE
                     SGRATAPVS"
     gene            8533..8916
                     /locus_tag="VF226_18540"
     CDS             8533..8916
                     /locus_tag="VF226_18540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008567285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7076041.1"
                     /translation="MDRKIWETGLAKRKAVLGEDYVNKSMSNADDFNRPFQDMLNEYC
                     WGTIWGDERIPAKTRSMINLGLIAGLNRMEEWELHLRGAIRNGVSREEIQSILHQIAV
                     YAGMPAGVECFRIARRVFAELDGKA"
     gene            9010..10086
                     /locus_tag="VF226_18545"
     CDS             9010..10086
                     /locus_tag="VF226_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7076042.1"
                     /translation="MAAKMKAAVFVEPNRIVLDEKPVPDIGPLDALLRITTTTICGTD
                     IHILKGEYPVERGLTIGHEPVGVIEKLGSAVKGYHEGQRVIAGAITPSGYSYACLAGD
                     CAQDGADTAHGWKPMGGWKFGNTIDGCQAEFVRVPDAMANLAPVPDGLSDIEVLMCPD
                     IMSTGIAGAESGNIKIGDLVAIFAQGPIGLCATVGARLMGATAIFAVDMLPERLEMAR
                     KLGADHCINSANTDPVDEIMRLTSGRGVDVAIEALGKQTTFEAALRVLRPGGTLSSLG
                     VYSKDLSIPLGPFAAGLGDHRIITTLCPGGKERMRRMMNVIGSGRADLKPLVTHSFKL
                     DDIEAAYDLFGHQRDGVLKVAITP"
     gene            complement(10319..10771)
                     /locus_tag="VF226_18550"
     CDS             complement(10319..10771)
                     /locus_tag="VF226_18550"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7076043.1"
                     /translation="MPENSVHERRSRADYNHWTTVTIRYCDQDPIGHINNAAMAAFLE
                     QARVALVYPTLKRFGGPDLELVIARLVIDYLNELHFPGSVDVGTRIARLGTKSFKLQH
                     GVFMAGDETCAALAECTMVFFDVRNRASVLPPPEVRAALEDFMRRQPA"
     gene            complement(10915..11595)
                     /locus_tag="VF226_18555"
     CDS             complement(10915..11595)
                     /locus_tag="VF226_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7076044.1"
                     /translation="MTTSTSSLRTVHLKLPASTEELRKLEIGTVAYLSGRIFTAREGV
                     YKRAVEDGYGLPAAPAELGSANFHCSPAASIAPDGSYTVGAVTATASFRFAKWLDGWF
                     KLSGINIVIGKGGMAGETYRSSFLPNNAIYLTTVGYGTGALLGRGIKRVAGVHWLEEL
                     GLAQAIWVLEVENFGPFLVESDLDGNSLFERENAKIASGLDQLYAGTRPATLRRYGET
                     DDKTEEVI"
     gene            complement(11663..12760)
                     /locus_tag="VF226_18560"
     CDS             complement(11663..12760)
                     /locus_tag="VF226_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7076045.1"
                     /translation="MRAAVLTEVNKPLEILDLEQEGPKQFEARVKVKAAGVCMSDWHI
                     MNGDWPLPLPMVLGHEAAGEVVECGPGVTNVKVGDHVIFSFRPHCGYCPYCSQGRTVL
                     CTGHNDSPRWRMHDGSSRVKLNGEPVNVMARIGTFSEYVVCPAEQLVSVRKDLPWTHA
                     AIIGCSVATGVGAVIRHAQVPAGSTVLVVGCGGVGLNVVQGAKLAGARMIIAADLLDN
                     KLQYAKQFGATHTINAREDDVVKRVRDLTGGLGADYAFDAIGTSTTAVQIVDAVAPAG
                     HAVLVGIPAYSATAPIGLANMVYMEKKLTGTYYGSIRPEIDFGILADLDLDKRLDLDG
                     LISRTYRFEEINEGFKLLTSGSVARGVIVFD"
     gene            complement(12847..13809)
                     /locus_tag="VF226_18565"
     CDS             complement(12847..13809)
                     /locus_tag="VF226_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX7076046.1"
                     /translation="MIPLTSIQSTAEQLMAKAAIEIPEDYLSGLRKCADTETGELSAF
                     VIKAMLENYEAAKEDRRAMCGDTGCPRWYVKIGNEARIEGGPIALEAALRRATAKATQ
                     DVPLRPNRVHPLWRTDHNNNVGINAPEIEYAFHPDADWIDLTTVHKGGLFGSDYRMLF
                     PGDGIDGIKRFYLDTLIAFGKRGLACQPAIVGIGLGGSKDTCMVLGKQASCLRTVGSR
                     NPDPKIAALEDELKTLGNSIGMGAMGFMGSSMVVACHIEVGYCHTGGMPMSVHMFCLS
                     SRRATARIHADGRAEFRTDPMWFTDYSRRSTVEWEQPHQVEAAE"
     gene            complement(13826..14059)
                     /locus_tag="VF226_18570"
     CDS             complement(13826..14059)
                     /locus_tag="VF226_18570"
                     /inference="COORDINATES: protein motif:HMM:NF019540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEX7076047.1"
                     /translation="MSSRLPALKARDILKALEKAGFVVVRSKGSHRICEHVQDPSRRT
                     VIPDHGPRDLPRGTVRSIIEQAGLSVEEFLDLL"
     gene            complement(14056..14298)
                     /locus_tag="VF226_18575"
     CDS             complement(14056..14298)
                     /locus_tag="VF226_18575"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX7076048.1"
                     /translation="MSQTQTYTVVLQPEPEGGFTVRVPALPEIVTYGADEEEALRMAK
                     EAIELSLEHRLSRGEQLPESGQAPVREVTVVIATAA"
     gene            complement(14324..16042)
                     /locus_tag="VF226_18580"
     CDS             complement(14324..16042)
                     /locus_tag="VF226_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7076049.1"
                     /translation="MRTDILILGSGGAGLFAALHAHQAAPHATITVATKGLLGKCGCT
                     RMVQGGYNVALHKGDSVERHFMDTIEGGKWLPNQALAWTLVTRAIERVRELENEIGCF
                     FDRNPDGSLHGKAFAGQTFDRTVHKGDLTGIEIINRLMEQVWARPIQRLEEHRAVALI
                     KDKAGEAIAGVLMIDIRTGTFRFVEAKAVLLATGGGPTMYRYHTPSGDKSMDGLAMAL
                     RAGLPLRDMEMVQFHPTGLLAGPDTRMTGTVLEEGLRGAGGHLLNGNMERFMERIDPK
                     AERATRDKVSQAIYAEMRAGRTTRNGGVYIKMSHLGPDKVRKQFKGMVERCADCGFDL
                     AGGLVEVVPTAHYHMGGVICDTDTSTELPGLFVAGEDAGGVHGANRLGGNGVANSTVF
                     GGIAGDTMPGFIARNPHWRAPDEEAIAAEMARATRPFEKRPGDLNRIREQLLDVMWDD
                     VGVIRDRAGLERAVARLDVIDEELHATGLRDGDRAFNLTWHDWLNLESLVAVSKVIAK
                     AALKRENSRGAHCREDFPEEGDLATSTFTVVRQRDGQVDVTDAPVAFTIVQPGETLLE
                     EMRAAE"
     gene            complement(16062..>16400)
                     /locus_tag="VF226_18585"
     CDS             complement(16062..>16400)
                     /locus_tag="VF226_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b subunit"
                     /protein_id="HEX7076050.1"
                     /translation="TTAHRRQGLWIAALVHRLSGIALACFLPLHFLALGLAIEGETRL
                     DGFLAWTEQPVVKLAESSLVFLLTIHLLGGLRILYVEAIGWRPVQKVLAVAIALAAAV
                     LAFIFLVRLV"
BASE COUNT         3067 a         5339 c         5166 g         2828 t
ORIGIN      
        1 tgccggacag caccgatgcc acgacgaggc catcgcgctg gacggccgcg gacacttcgg
       61 gcagcagcgg tccggaattt ccgatgcagg tggtgcaacc atagccgacg agatcgaagc
      121 cgagcgcttc caggaacgga gtgagcccgg cctgatcgta gtattccgtc accacccgcg
      181 agcccggggc gagtgaagtc ttcacccatg gctgccgccg gagcccgcgc tcgacagctt
      241 tttttgcaag caggccagcg gcgatcatca cggatgggtt ggaggtgttc gtgcagctcg
      301 taattgccgc gatggccacc gatccgtggc gcaggccctt gatggcgtcc tcctgcggtc
      361 gctccagggg cacatcctct ggactgccct ccccccagac gccgactctc gaaccgtcgt
      421 tgcccttggc ggcagcttgc ttctgcgcag ccggcggctt gggcggcatc agcgacggca
      481 gtgcgtcgtg aaaagcctgc ttcgccttgc gcaacggaat gcggtcctgc ggccgccctg
      541 gacccgcgat gctaggctcg acggtcgaca ggtccagctc gagcgtgtcg ctgaacacag
      601 gctccggcgc attgagatcg tggaagaggc cctgctcctt catgtaggct tcgacgagcg
      661 cgacatgctc gccggtgcgc cccgtaaaac gcagataatc cagcgtcagc tcgtcgacgg
      721 ggaatatcga gcaggtcgat ccgtactccg gggacatgtt gccgatggtg gcgcggtccg
      781 tgacaggcac gttgcggacg ccggggccgt aaaactcgac gaagcgcccg acgacgccct
      841 tcttgcgaag catctccgtg accgtcagca ccaggtcggt cgccgtcgca ccctcgcgca
      901 actcaccgtg gagccggaat ccgaccacat cggggatgag catcgaaatc ggttgcccca
      961 gcatggccgc ttcggcctcg atgccgccga cgccccaacc cagaacgccc aggccattga
     1021 ccatcggcgt gtgcgagtcg gtgccgacca gcgtgtcggg ataggccatc ggcttctccg
     1081 tccctgcggg cacgtcgccc tcgcgcgcac cgaacacgac gcgcgccaga tactccagat
     1141 tgacctgatg gcagatgccg gcattgggcg ggccgacgcg gaaattgtcg aaggcttgct
     1201 gcccccactt caggaaggcg taccgctcgc cgttacgttt gtagtcgagc tcggtgttga
     1261 tgcgcatggc atccgggcgg ccgaacgcgt cggcctggat cgaatggtcg atcaccagct
     1321 ccagcggctg cagcggatta atcttcgccg gatcgccgcc gagccgagcc atcgcgtccc
     1381 gcatagcggc gaggtcgacg atggccggca cgccggtgaa gtcctgcatc aacacgcgcg
     1441 ccggcgtgaa ggcgatctcc ttctggccgt ctgcgtaggg tttccagccc gccaacgcct
     1501 cgatatccgc tttgttgacg gttgtgccgt cttcgttgcg cagcaggttt tcgagcagga
     1561 tgcgcagcga aaagggcagc cggtctacat cgaaaccgtg ctggcccagt ttcctgaggc
     1621 tgaagaacct gaacgtctta tccccaacgc gaagctcgga agcagtgccg aagctgtcca
     1681 tggcttgctc ctctcgggcg gctcgtgccg catcaaaaat tcggtgccgc agccgtcatc
     1741 cactctgaat ggcacggctc gacggatcaa cgggacgcga cctgaggggt tccgcggcca
     1801 gccgccgctt gcacaggtga ctcgcgtgcc gcccgctcac agtagctgaa aacactttgc
     1861 gtgtagaaat tcagggtgtg ggcggatatc ccttatgccc ttgccgctgc cagcttgagg
     1921 gagtccaaca caatgatgaa gcgtcgcctc ggtgcgaatg gtccgctcgt ctctgcaatc
     1981 ggacttgggt gctggagctt tgccggcgcc tacggaccaa cgacggaggc cgagagccac
     2041 gccacgctga gccgtgccat cgagctcggc gtcgacttcc tggatactgc cgacgtctac
     2101 ggccatggga ccagcgagga ggtgatcggc cgcttcctca agcaaaaccc ttcggcaaag
     2161 gtcgtgattg ctaccaaggg cggcatcaag cgccggccag gctcggcgga acggaccttc
     2221 gacaacagcc cagaatatct gcgatcggcg ctcgaggggt cgctgcgccg gctagggcgg
     2281 gactacgtcg atctctacta cgttcatcgc cgggaccaga gccgtcctgt cgaagaggtc
     2341 gccgacacgc tcgccggttt cgtgaaggaa ggcaaaatcg gagggttcgg cttctccgag
     2401 atctcgcccg ccacgctgag gcgtgctcac gcggtgcatc cggtcatggc ggtgcagagc
     2461 gagtactcgc tgtggtcgcg cttgccggat ctcggaatga tcgaagcctg ccgcgagctg
     2521 ggcgtcgcct tcgttccgtt cagcccgctc ggacgcggat tcctgaccgg aacggtcact
     2581 cgaacggacg acttcgggcc gaccgacttc cgcaaaccca atccgcgttt tctggagccc
     2641 aatttctcgc tcaaccggaa aacgctggag ccattcctgg ccctcgcgag ccgctatgac
     2701 gcctcccccg ggcagcttgc gctggcgtgg gtgctggcgc aaggcgacca ccttgtcccg
     2761 atcccgggca cacggtcggt gaagcatctg gaagagaacg ccgccgcagc ctcgatcgcg
     2821 ctcagcagag agcaggttgc cgccatcgag cgcgcgctgc cccgcgggtt tgcgcatggc
     2881 gatcgttatt cagatcagca atggatcggc gccgagcgat acggctgaac ggagccggcg
     2941 gctttagaga cgcagcctga ccggggtcag gctcaacggg tccgagacat cgcggatgcg
     3001 cacgccggtc cgctcctttg ccagctgggc ctcacggcgg gtgatgtagg ccgccgagct
     3061 gaagatgatg agggcgccga cccaggtcca caggtcggtg ctctcgccga gcagcaggta
     3121 gcctgccgcg acggcgaacg gcagccgcga gaactcgaag gtgaagacca gcgaggcgtc
     3181 catcgacgca aatccgcgaa gcagtgcgac gtgaccgagc acggcgcaca ggcccaggcc
     3241 gatgaggtac ggccacacgg ccagggccgg ccattcccac acgaacaatg ccgggatcaa
     3301 ggaggccgga gtcgcccaca aattcgtgat gaacacgatt ttgtcggggt cgtcctgggc
     3361 ggtcatctgc ttcaacaggg gtccgacgat accggccgtc agtgcggaga agagcgccag
     3421 catctggcca aggccgaaac ccacgccgcc cggccgcagg atgatgagcg cgccgatgaa
     3481 gccgatgacg agcgcggtcc agcggcgcgc ccgaaccact tcaccaagcc agaacacggc
     3541 gaaaatggtc gcgaacaagg gcgcgagaaa gcccaccgcc gtcaactcgc ccaatgggat
     3601 gagcgaaagc gccgaaaacc acgccatcat ggaaaggaac gcgagcccaa cgcgcacgcc
     3661 ataaaggcgg aactgcgcgg agacggccaa cgatggccct cgccagtaca gcagcggcag
     3721 catcaggacc aggcagaaga agttgcggaa gaagatcacc tgcagcggat cgaccccctc
     3781 gtgcatggcg aatttgccaa aggtgccgag accagcgaag gtcgccatgg cgatcgtgac
     3841 ccagatcgcg cctgcaatcg ggtcgttgcg cttccccggc ttgccactgg cccacagccg
     3901 ggcaaccagc ccaccgatgt ttcctccgaa cgacgacaac tgattatgtc cttctgttcg
     3961 ccctctgccg gggcaactga agatgcgctg caaagccgtt tcatgacaag gctgttggcc
     4021 atacgcacat gtcggttatg ctaccccgtg ccccccgctg gactgcggcg caggcgtagg
     4081 ccaatgttcg gcccccaatt catggaactc gaggcaggcg atgagcggaa agacttcggc
     4141 gggaaccaga tcgggtgcca tcgggcgtgc acgcagctac ctcgagaatg gacagttcga
     4201 agccgatctg gcgcgccggg ttgcttatcg gaccgaaagc cagctcctgc ccgaaagcct
     4261 tccgcagctc gaacgctacc tccgtgaaga aatggagccg gcgttcgccc gaatgggctt
     4321 tacctgccgc atttaccaga accccattgc cggccagggc ccggtcctgc tcgccacgcg
     4381 catcgaggac ccgagcctgc caaccgtact ggggtacggc catggcgatg tcatccgcgg
     4441 cctcgatgag caatggacca agggcaatgg cccctgggtg acggcgcggg agggagaccg
     4501 gctctatggc cgtggcacgg cggacaacaa gggtcagcac acgatcaaca tggcggcgat
     4561 gcagtccgtc atggacgagc gcgggggccg gctcggcttc aatgccaaat tcatgatcga
     4621 aatgggcgag gaagcaggct ccaaaggcct gaaggagctg gtcgaggcac accgcgagga
     4681 tttcgcggcc gacgtgttga ttgcctcgga tggaccgcgg acgcgccctg agcgcccgtc
     4741 actcaacctc ggctgccgcg gcaccatcaa cttcgatctg gtctgcgagc tgcgggacgg
     4801 cgggcatcac tccggaaact ggggcggcct gctggccaac cccgccatca ttctcgccaa
     4861 tgccctcgcg acgatcgtcg gcccaaccgg cgcgcttctg gtgaaagagc tgaaagcgcc
     4921 ggccatgacg aaggccgtgc gcgaagcttt ggcagacgtc gagatcgatg gcggcgacaa
     4981 gggccccaag gtcgacgagt ggtggggaga gccgggccac acgccggccg agagggtcta
     5041 cggatcgaac gtattcaacg tgctggccat cggggccggg atgccgggcc ggccggtcaa
     5101 tgccattccg ccccgggccg tcgccaactg ccagcttcgc ttcgtcgccg gtacggatgt
     5161 tgcgggcgtc cgccccgcgg tcgagcggca tctgcgcgag aatggcttcg ataccgttcg
     5221 cgtcgccgat ccacctgccg ccaatgacgg cagcttcccg gcaacccgca ccgagcccga
     5281 cgacccctgg gtggtctggg cggtcaattc cttccagcgg acgtcgaaca tcaagcccgc
     5341 cgtggttccc cagatgggcg gctcgatctg caacgacatt ttcaccgacg tgctcggtat
     5401 gccgacactc tgggtgccgc attcctatgg ctcctgctcc cagcacgcgc cggatgagca
     5461 catcctcatg tcggtctcgc agagcgcgct cgagctgatg gcggggctct attgggatct
     5521 cggcgaggcc ggagttccag cgcgatcctg aaggccccgc gccagtcggc aatagcaacc
     5581 cacttttgaa aggcacctcc ccgatgaccg aaccctgcga cctgtccgcc gtcgaggccc
     5641 gccggctgat cggcaacaag aagctctcgc ccgtcgagct gctggagagc tgcctgaagc
     5701 gcattgagaa ggtcaatccg accctcaacg ccatcgttgc gatggacgag aaggctgcaa
     5761 aggacgccgc gaaagccgcc gaggaggcgg tcatgcgggg cgacgacctg gggctgctcc
     5821 atggcctgcc gatcggcatc aaggatcttc aggccacggc tggactgcgg accacgtggg
     5881 gctcgctgat ctacaaggat catgttccgg aaaaggacga agcgacagtc gccaatctga
     5941 gggatcaggg cgccgtcatc ctggccaaga cgaacacgcc ggagttcggc gccggcgcca
     6001 acacgaaaaa ccgggtctat ggcgcgacgg gcaacccctt cgatccgaac aagacctgcg
     6061 ccggctcgtc aggcggatct gctgtggcgc tcgcgaccgg aatgctgccg ctggctaccg
     6121 gctccgacta cggcggcagc ctgcgcacgc ctgccgcatt ctgcggcgtc agcgggttcc
     6181 ggccttcacc gggcgtcgtg ccgggtccgg agcggtcgag tgccttcacc ccattctcgg
     6241 tcaacggtcc catggcccgc tccatcgccg acctgcatct gctgctcaag gcgcagatcg
     6301 atctcgacaa gcaggacccg ttttcgagcg acgatgcact gcgcatcccc gaggagctgc
     6361 cccagctcga cctcggcagc ctgcgcgtgg cgatctccac cgacctcggc tgcgcgccgg
     6421 tcgacaagga catccggcgc attttccagg agcgggtgag cacgtttcgg cacgtcttcg
     6481 cggaaaccca ggaccgcgat ccggatctcg gcccggtgca tgacgtgttc gagatcctgc
     6541 gcggggtcta cttcgtcggc gcgcatcgcg agcggctgga gaagcaccgg gatctgctcg
     6601 gccccaacgt gatcgacaat accgagcgcg ggctcaaatg gaccgcagcg gacatcgcct
     6661 gggccaatgt cgagcagtcg aagatctatc gccggttcct cgatttcttc gacgatgccg
     6721 acgtgctcat ctgccccgca gcctcggtca ctccgtttcc gcacagcgag ctgtccgtca
     6781 cggagatcaa cggcgagaaa atgccgacct acatgcgctg gctcgcaatc acctacgcac
     6841 tgacaaccgc cgttccttcg gctgccgtca tcccctgtgg cgtcgaccat gtcggcatgc
     6901 cgttcggcat ccaggtcgtg ggcccgaacg gctcggatgc gctcgtcctg gcagtggcca
     6961 acgcgctgga gcgggtgctc gccgagaacc cggcaacctc gcggcctgtg cccgacatcg
     7021 cggcgctgac tggctgacct gttcagctca cgggagttca gctcacggga gcagtggcgc
     7081 ggccgctttc ggtgcggctg agccacaggg caccgagcag tacgactccc atcatcggca
     7141 gcgccgcgag gttgaccacc gtccagccga cattcgcctg caggactccg gaaaggccgg
     7201 ccgcggtcgc cgtggtgccg taaaccgcga agtcgtggct cgcctgcacc ttggcccgct
     7261 cggcgggtgt ataggtcgtc gtgagcagcg acgtgccgcc gacgaacgtg aaattccagc
     7321 caaggccgac gagggcgttc gccaggaaga agttccagaa cgcgatgccg gcgagattga
     7381 ttatggcgca cgccatctgg atcagcgcgc ccgtggcgat gatcggtagc acgccgaacc
     7441 gcgcaatgag atggccggtg aagaacgagg gcaggaacat cgcgatcacg tgtgcctgga
     7501 tgaccgtcgc gctgtcctgg aattcgaagc cgcaagcgag catggcgagc ggcgtggcgg
     7561 acatgacgag cgtcatcacg ccgtaaccga acatcgatga cgtgagcgcc acgatatagg
     7621 ctggttgccg cacgatctgg cgcatcggcc ggccgccttg ggccttctcg gcagctgtta
     7681 accccggaat acggagtccc tgcacaagca ccagcacgaa cgcggagtag accgcgacca
     7741 tcacgtagac gccaccgaac acgaacggat cgagccagtg cacggaccac ttcgcggtct
     7801 gcggaccgag gaacccggcg atcacaccgc cgagcatgac gagcgagatg gccttgggac
     7861 gcagttcagg ctcggtggca tcggccgctg caaaacggaa ataccacgcg aaagcggctg
     7921 ccattccctg gaacagagag ccgacgcaga gcaggacgaa gctttgcagg aaaatggccg
     7981 cgagagagat gaggccggaa gtgacgccgc acatcgcgcc gaccgagaac ccgccgcgtc
     8041 gtccgatccg ggccatcagg aacgacgcgg ggatggtcgt catcatcacc ccggcgtggt
     8101 tcaggaacag agggaaggtc gacagcgtct tgtcgacgcc gagcagcgcg tatcccgcga
     8161 gcggcgtcgt agcaatggcc atcgactgca cgcacatgaa gaagccctgg ctgaacgcca
     8221 gcagcgctac gttgcgcggg ttcggtatgc cgaaaaagga catgcgctct tgcgggtgtg
     8281 aagacggctg cgacaccaaa ggtccatcaa gcgcgccaca atgccttgtt tcgccgcccg
     8341 cgcaaaggag ggaatcgcga ccgcttcttc cagctgcccg tgcccagagc gggtaccagg
     8401 agtttgcctc tgcctcgccg gcgtgtaatt tccgcgtcgg ctggccggca atccgagcgc
     8461 ggcaccggtt ttttaccttc gctcttgccc gcccgtggcc gcgtttcagc agcgtaacca
     8521 ggaggttcac gaatggatcg gaaaatctgg gagacgggcc tcgccaagag gaaggcggtc
     8581 ctcggcgagg actacgtgaa caaatccatg tcgaatgcgg acgacttcaa tcgtccgttt
     8641 caggacatgc tcaatgagta ctgctggggc accatctggg gagacgagcg cattccggcc
     8701 aagacccgct ccatgatcaa cctcggcctg atcgccggcc tcaaccgtat ggaggaatgg
     8761 gagcttcacc tgcgcggcgc gatccgcaac ggtgtctcgc gggaggagat ccagtccatc
     8821 ctgcatcaga tcgccgtcta tgccggcatg ccggctgggg tcgagtgctt ccgtatcgcg
     8881 cggcgcgtct tcgcagagct cgacggcaag gcctgaacga gccctcgcgc ggcagcctgt
     8941 ccctgctctc gccaggtccg gaacgatccg cccggccgat ggttcagcgg cgaaaaggga
     9001 gcttggcgaa tggctgcgaa gatgaaggct gcggttttcg tcgagccgaa tcgtatcgtg
     9061 ctcgacgaga agcctgtccc ggacatcggt ccgctcgatg ccctgctgcg gatcacgacc
     9121 acgaccatct gcggcaccga catccacatt ctcaaaggcg aataccccgt cgagcggggc
     9181 ctgaccattg gccacgagcc ggtcggcgtc atcgagaagc tcggctcggc ggtcaagggc
     9241 taccatgaag gccagcgggt gatcgccggc gcgatcacgc cgagcggcta tagctatgcc
     9301 tgcctcgcgg gtgattgcgc acaggatggc gccgacaccg cccacggctg gaaacccatg
     9361 ggcggctgga aattcggcaa caccatcgac ggctgccagg ccgagttcgt gcgagtgccg
     9421 gacgcgatgg cgaacctcgc gccggttccg gacggtcttt ccgatatcga ggtgttgatg
     9481 tgcccggaca tcatgtcgac tggcatcgcc ggtgcggaat cggggaacat caagatcggg
     9541 gatctcgttg cgatcttcgc ccaggggccg atcgggctct gcgcgaccgt cggcgcgcgg
     9601 ttgatgggcg cgacggcgat ctttgcggtc gacatgctgc ccgagcgcct ggagatggcc
     9661 cgcaagcttg gagccgatca ctgcatcaat tcggcgaaca ccgatccggt cgacgagatc
     9721 atgcggctga cgagcggccg cggcgtcgac gtcgccatcg aagcccttgg caagcagacg
     9781 accttcgagg ccgcactgcg tgtgctgagg cccggcggga cactttcgag tctcggtgtg
     9841 tacagcaagg acctgagtat cccgctcggc cccttcgctg ccggacttgg agatcaccgg
     9901 atcatcacga cgctctgccc cggcggcaag gaacgcatgc ggcgcatgat gaacgtcatc
     9961 ggctcgggcc gcgcggatct caagcctctc gtcacacaca gcttcaagct cgacgacatc
    10021 gaagcggcct atgatctttt cggccatcag cgggacggtg ttttgaaagt cgcgatcacc
    10081 ccgtgatttg cggcagagct gcagcttgac tgctgcgggt caaggtcgat cgtcgtaaga
    10141 catggatgtg aatggaagcg ccccggagag ctgcgccgcg ccaccccccg gggctttcgc
    10201 cccgctgcct gaagctcgca atcacgcggc tggcaaaacg taaatgctcg agcgcttgct
    10261 tggttccggt ctcgcgcgga aagtgtattt tgcgcggctg atccgcaaca cattcttctc
    10321 acgcgggctg gcgtcgcatg aagtcttcca gagcggcccg aacctctggg ggagggagca
    10381 ccgaggcccg gtttcgcacg tcgaaaaaga ccatcgtgca ctcggccaaa gccgcgcacg
    10441 tctcatcgcc ggccatgaac acgccatgct gcagcttgaa cgacttggtg ccgagccggg
    10501 cgatgcgcgt cccgacatcc acgctgcccg gaaaatgcag ctcgttgagg tagtcgatga
    10561 cgagccgtgc gatgacgagc tccagatcag ggccgccgaa acgcttcaac gtcggataca
    10621 cgagagcaac gcgggcctgc tcgagaaaag cggccatggc tgcgttgttg atgtgtccga
    10681 tcggatcctg gtcgcaatag cggatcgtga ccgtggtcca gtgattgtaa tcggcgcgac
    10741 tgcgccgttc gtgcaccgag ttctccggca tgtgcttccg atgggctcga accgacttct
    10801 ggtgaccgca tgatgcgttc ggcacgcgat gcgcaagaga cctacgtggg cgaggcgcgc
    10861 gctgtcaaag acgtacagca gatctgaggc aattcgccga acgacgccgc cgattcagat
    10921 gacttcttcc gtcttgtcat cggtctcgcc ataacggcgc aacgtcgccg ggcgggtgcc
    10981 tgcatacagc tggtcgagcc ccgatgcgat cttcgcgttt tcacgctcga acaggctgtt
    11041 gccgtcgaga tcactttcca cgaggaacgg gccgaagttc tcgacctcga gcacccagat
    11101 ggcctgggca agtcccagct cttccagcca atgcacgccc gcaacccgct tgatcccgcg
    11161 cccgaggagc gcgcccgtgc cgtaaccgac tgtcgtcagg tagatagcgt tgttgggcaa
    11221 gaacgagctc ctgtaggtct cgcccgccat gccgcccttg ccgatcacaa tgttgatgcc
    11281 ggagagcttg aaccagccgt ccagccattt ggcaaaacgg aacgaagccg tcgcggttac
    11341 ggcgcctacg gtataactgc cgtccggcgc gatgctagcc gcgggcgagc aatggaaatt
    11401 ggcgctgccc agttcagccg gagcggcggg cagcccgtat ccgtcctcga cagcccgctt
    11461 gtagacgccc tcgcgcgcgg tgaagatgcg gcccgagaga taggcgaccg tgccgatttc
    11521 gagcttgcgc agctcctctg tcgaggccgg aagcttgaga tgcacggtcc tgaggctgga
    11581 tgtgctggtc gtcatcggta cgccttcgct ctcttgggcc gttcgacagc agatctcgct
    11641 ctctcgaagc gccgctcagc gctcaatcga aaacgatgac cccgcgcgcg acactccccg
    11701 atgtcagcag cttgaagcct tcgttgattt cctcgaaccg gtaagtccgg ctgatcaggc
    11761 catcgagatc gaggcgcttg tcgaggtcta gatcggccag gatgccgaaa tctatctctg
    11821 gacggatcga gccgtagtag gtcccggtga gctttttctc catgtagacc atgttcgcca
    11881 agccgatggg cgccgttgcg ctgtaggccg gaatgccgac caacacggca tgacccgctg
    11941 gcgcgacggc atcgacgatt tgaacagccg tcgtggaggt gccgatggca tcgaatgcgt
    12001 aatcggcacc gaggccgccc gtcagatcgc gcacgcgctt caccacatcg tcctcgcgcg
    12061 cgttgatcgt gtgcgtggcg ccgaactgct tcgcatactg cagcttgttg tcgagcaggt
    12121 cggccgcgat gatcatccgt gcgccggcaa gctttgcgcc ctggaccaca ttcaatccca
    12181 caccgccgca gccgacgacg agcaccgtcg agcccgcggg aacttgcgca tgacggatca
    12241 cggcgccaac gccggttgca acactgcagc cgatgatcgc cgcatgcgtc cacggaaggt
    12301 ccttgcgcac cgagacgagc tgctcggccg ggcagacgac atactcggag aatgtgccga
    12361 tccgcgccat cacgttgacc ggctcgccgt tgagcttcac gcgcgaactg ccgtcgtgca
    12421 tgcgccagcg tggcgagtcg ttatggcctg tgcaaagcac cgtgcgccct tgcgagcagt
    12481 aggggcaata cccgcaatgc ggacggaacg agaagatgac gtggtcaccg accttcacat
    12541 tggtcacgcc gggcccgcat tccaccacct cgccggccgc ctcgtggccc agcaccatcg
    12601 ggagaggcaa tggccaatcg ccgttcataa tatgccagtc gctcatgcaa acgccggctg
    12661 ccttgacttt gacgcgggct tcgaactgct ttgggccctc ctgctccaag tcgaggattt
    12721 caagcggctt gttgacttcg gtcaggacag cggcgcgcat agtcgtttct cccgaaagtt
    12781 cgattcattc accatctgaa atcgcgcgat cgaaatcaat cgcatcgaac cgctcatctc
    12841 cgcgacttat tccgccgctt ccacctggtg cggctgctcc cactcgacgg tcgaccggcg
    12901 cgagtaatcc gtgaaccaca tcggatcggt gcggaactca gcgcgcccgt ccgcatggat
    12961 ccgcgcggtg gcgcgccgtg atgacaggca gaacatgtgc acgctcatcg gcatgccacc
    13021 ggtgtggcag tagccgacct cgatatggca ggcgacgacc atggatgaac ccatgaagcc
    13081 catcgccccc atgccgatcg agtttccaag cgtcttcagc tcgtcctcga gcgccgcaat
    13141 tttcggatcg gggttgcggg agccgaccgt ccgcaggcat gaggcctgct tgccgagcac
    13201 catgcaggtg tccttggacc cgccgaggcc gatgccgacg attgccggct ggcaggcgag
    13261 gccgcgcttc ccgaaggcaa tgagcgtatc cagataaaag cgcttgatgc cgtcgatccc
    13321 atcaccggga aagagcatcc ggtagtccga gccgaacagg ccacccttgt ggactgtcgt
    13381 caggtcgatc cagtccgcgt cgggatgaaa cgcatattcg atctccggcg cattgatgcc
    13441 gacgttgtta ttgtgatcgg tccgccagag cggatgcacg cgattaggcc gcagcggcac
    13501 atcttgcgtg gctttggcag tggcccggcg caaagccgct tcgagcgcaa tcggcccgcc
    13561 ttcgatgcgc gcctcgttgc cgatcttcac gtaccagcgc ggacagccgg tatcgccgca
    13621 cattgcgcgc cgatcctcct tcgccgcctc gtaattctcg agcatagcct tgatgacgaa
    13681 cgccgacagc tcgcccgttt cggtatcggc gcatttccga aggcccgaaa gataatcctc
    13741 cggaatctcg atggccgcct tggccatgag ctgctccgcg gtggattgaa tggaggtgag
    13801 ggggatcatg gaacctgccg ttgggctaaa gcaaatccag aaactcttct acgctcaatc
    13861 ctgcttgctc gataatgctc cggaccgttc ccctcggcag atctctcggc ccgtggtcag
    13921 gaatgacggt tcgacgcgaa ggatcctgga cgtgttcaca tatgcgatga ctgcctttcg
    13981 aacgaacaac cacgaatcca gccttctcga gagccttcag gatatcacgc gccttgagcg
    14041 cgggaagacg actgctcatg cagccgtcgc gataacgacc gtgacctccc gaacgggtgc
    14101 ctgtccggat tcgggcagct gctcgccacg gctcaagcga tgctcgagtg acagctctat
    14161 cgcttccttc gccatcctca aggcttcttc ctcgtcggcg ccataggtca caatctcagg
    14221 gagtgctggc acgcgaaccg taaaccctcc ttcgggctcg ggctgcagca ccacagtata
    14281 cgtttgagtt tgactcatag ctcaatgccc agtgtcagag ctcctattcc gcagcccgca
    14341 tctcctcaag cagcgtttcg cccggctgga cgatcgtgaa cgccacgggc gcgtcggtca
    14401 cgtcgacctg accgtcgcgc tggcggacga ccgtgaaagt ggatgtggca aggtcgcctt
    14461 cctcagggaa gtcctcgcgg caatgggcgc cgcgtgaatt ctcgcgcttc aatgcggcct
    14521 tcgcaatgac tttggaaacg gcaacgaggc tttccaaatt cagccagtcg tgccaggtca
    14581 gattgaaagc gcggtcgccg tcgcgcaatc cggtcgcgtg gagttcctcg tcgatgacgt
    14641 cgagacgggc cacagcgcgt tccagcccgg cccgatcacg gatgacaccg acatcgtccc
    14701 acatgacgtc gagaagctgc tcccgaatcc ggttgagatc gccggggcgt ttctcgaagg
    14761 gtctggtggc ccgcgccatt tctgcagcga tcgcctcctc atccggtgcc cgccaatgcg
    14821 gattgcgggc gatgaagccc ggcatggtgt cgccggcaat gccaccgaac acggtggagt
    14881 tcgcaacgcc gttaccgccg aggcggttgg ccccgtggac gccgccggcg tcctctcccg
    14941 ctacgaacaa ccccggcagt tccgtcgagg tgtcggtgtc acagatgacc ccgcccatat
    15001 gatagtgcgc cgtcggcacc acctccacga ggccgccggc gagatcgaat ccgcagtcgg
    15061 cgcatcgctc gaccatgccc ttgaactgct tgcgtacctt gtccggcccg agatggctca
    15121 tcttgatgta aacgccgccg ttccgcgtcg tgcggcccgc acgcatctcg gcatagatcg
    15181 cctggctgac cttatcgcgc gtcgcccgct cggccttcgg gtcgatgcgc tccataaagc
    15241 gttccatgtt gccgttgagc agatggccgc cggcaccgcg caggccctcc tcgagaaccg
    15301 ttcccgtcat gcgcgtgtca gggccggcga gcaatcccgt cggatggaac tgcaccattt
    15361 ccatgtcgcg cagcggcagc ccggcccgca gggccatggc gagcccgtcc atgctcttgt
    15421 cgcctgacgg ggtgtgatat cgatacatcg tcggcccgcc gccggtcgcc agcagcacgg
    15481 ccttggcctc gacgaagcgg aatgtcccgg tgcggatgtc gatcatcagc acaccggcga
    15541 tcgcctcgcc cgccttgtcc ttgatgagcg cgacggcgcg gtgctcctcg agccgctgga
    15601 tcggccgcgc ccacacctgc tccatcagcc ggttgatgat ctctatgccg gtgagatcgc
    15661 ccttgtgcac cgtacggtcg aaggtctggc ctgcaaaggc cttgccgtgc agcgagccat
    15721 cgggattgcg gtcgaagaag cagccgatct cgttctccag ctcacgaact cgttcgatgg
    15781 cgcgcgtcac cagcgtccag gcgagggcct ggttcggcag ccacttcccg ccctcgatgg
    15841 tgtccatgaa gtgccgctcg accgaatcgc ctttgtgaag cgcgacgttg tagccgcctt
    15901 gcaccatgcg cgtgcagccg cacttaccga gcaacccctt ggtcgccaca gtgatggtcg
    15961 catggggcgc cgcctgatgc gcgtgaaggg ccgcgaacag tcctgccccg ccggagccga
    16021 ggatcaggat gtcggttctc attcgttcga gcatgctggc ctcagacaag gcgaacgaga
    16081 aagatgaaag cgagcaccgc agccgcaagc gcgatggcca cagcaaggac cttctggacc
    16141 ggccgccaac cgatggcctc gacgtaaaga atgcgcaggc ccccgagcag atggatcgtc
    16201 agcagaaaca cgaggcttga ctccgcgagc ttgacgaccg gctgttccgt ccatgcgaga
    16261 aagccgtcga gacgcgtttc gccctcgatc gcaagtccga gcgccaggaa gtgcagcggc
    16321 aggaaacagg cgagcgcgat tcccgacagg cgatgcacca gggcggcaat ccaaagccct
    16381 tgacgccggt gagccgtcgt
//
LOCUS       DASVDU010000278         4916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1219622, whole genome shotgun sequence.
ACCESSION   DASVDU010000278 DASVDU010000000
VERSION     DASVDU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219622"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF226_18590"
     CDS             <1..283
                     /locus_tag="VF226_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX7076051.1"
                     /translation="LDNEHFGETGRQAGLTGNKVDLTAVARGFGIERTMTVTEEGKVG
                     ELAEFLFQGPGPAFAVAKIALSEDPWRLPVKDGGTIAHRFRVALGVETA"
     gene            503..991
                     /locus_tag="VF226_18595"
     CDS             503..991
                     /locus_tag="VF226_18595"
                     /inference="COORDINATES: protein motif:HMM:NF024671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HEX7076052.1"
                     /translation="MTQYYICKVLYFADKWHCLDWGRTITGDRYVAMDHGPVPSRVYE
                     MLKPASGEEDDLLDAVSSRLRFEVSGNKIQVFSRQLNDFSALAQSDKEYLDKAISFCR
                     PLRFGELREIAHQELSWIRATEHATSNNPPMDLADWFEQEEMDRDMAAQELLENVRYG
                     VA"
     gene            complement(1500..2930)
                     /locus_tag="VF226_18600"
     CDS             complement(1500..2930)
                     /locus_tag="VF226_18600"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7076053.1"
                     /translation="MYENLELYIDGKFTKGDSGVTSDVVNPSKDTVLGKLPHAKKAEL
                     DAALAAADKGFKEWKRVSAYERAKILRKAADLIRARADDIAKVLTQEQGKILIEARLE
                     VLGAADITDWFAEEGRRAYGRIIPARADGVRNMVIMEPIGPVAGFAPWNFPVTQAVRK
                     IAAALAAGCSIIIKCPEETPGSPIGLVRCFHDAGVPAGVINLVYGTPAEISEYLIPSP
                     IIRKISFTGSVPVGKHLNALAASHMKRATMELGGHAPVLVYDDVDTEHVAKLMAAMKY
                     RNAGQVCISPTRFFLQEKIYDKFVAKFTDIAKNLKVGDGFDPESKMGPLANPRRVNAI
                     EQFVQDAQEKGAKVATGGKRIGNQGNFFEPTVLTDVPDNARIMNEEPFGPVAVMLRFK
                     DTEEVLPRANKLPYGLASYAFTRNGKTATQIADALDSGMVTINHHGLALPETPFGGVK
                     DSGFGYEGGTEGLLVYMTGKFVSYMG"
     gene            complement(2994..4109)
                     /locus_tag="VF226_18605"
     CDS             complement(2994..4109)
                     /locus_tag="VF226_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX7076054.1"
                     /translation="MSDSALFSPARLRDLKLDNRIVVSPMCQYSAEDGSATDWHMMHL
                     GMLSNSGAGLLIIEATGVERAGRISPGCLGLYSDENEQALKRVVDACRKYGSAKIGIQ
                     LGHAGRKASTHRPWQGTPPGGPLKEGAWQTKAPSAIPFNEGWHVPQALTPQGIEELIE
                     AFVAAARRADRIGLDLIELHGAHGYLLNQFLSPLSNKRDDEWGGSLENRMRFPLEVFK
                     ATRAAWPESKPLGMRISAVEWVEGGWTIEDSITLAKRLKDLGCDYIDVSSGGNHPGQR
                     IQVGPGYQVPFAEQIKKAVGIPVMAVGMITEAEQAEGIIRSGQADFVAIARAFLDDPH
                     WAWHAAYRLGTEPKWPDQYRRVGPQLWPPAKRYQANA"
     gene            4413..>4916
                     /locus_tag="VF226_18610"
     CDS             4413..>4916
                     /locus_tag="VF226_18610"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076055.1"
                     /translation="MTESTETSLHGRSDGRDHGPDAGSATASSESTQLGAMGKAFTIL
                     EAIAAAKQPMTMSELVRACRLTKPTAHRITTMLVEMGYLEKEPLKRGLIEGPRLISLA
                     LTTLAAAAPRSRRHAILRSISEQTGETCNFGVLAGSEVIYLDRVEAKWPLALRFEAGS
                     RVPAHCTA"
BASE COUNT          931 a         1511 c         1480 g          994 t
ORIGIN      
        1 gctcgacaac gagcacttcg gcgagacagg tcggcaggcc gggttgacag gcaacaaagt
       61 cgacctcacc gccgtcgccc gcggcttcgg catcgagcgc acgatgacgg tcacggagga
      121 ggggaaggtc ggcgagctgg ctgagttcct gtttcagggc ccgggaccgg cattcgcggt
      181 ggccaagatc gcgctgtcgg aggacccgtg gcggctgccg gtgaaggacg gcggcaccat
      241 cgcccaccgg ttcagggtgg cgttgggggt ggagacggcg tgagtcgctt gcttgctgtt
      301 actttcagtg accgacgttc ttgtgtcgat aatggccctg ccctacattt tgcacttgac
      361 tttgtgatag aatgagactc agacgagtcg atcatttagc ctgggggctc ttatgagcac
      421 aaggcaacgc gtctttcgtc tccgattgga ccgtgacaag gcagtccaag cggtagacta
      481 tattgcgcag caatggcctg gggtgacaca gtactacatc tgcaaggtct tatactttgc
      541 agacaagtgg cattgcttag attggggccg caccatcacc ggtgatcgct acgtcgcgat
      601 ggatcatggc ccggtaccgt cgcgcgtcta cgaaatgctt aagcccgcct ctggcgaaga
      661 agacgatctg cttgatgcgg tttcgtctcg attaagattc gaagtttctg gaaataaaat
      721 ccaggttttc tctcggcagc tgaacgattt ttctgctctc gctcagagcg acaaggagta
      781 tctggataag gcgataagtt tctgccgccc tctgcggttt ggcgagctca gagagatcgc
      841 acatcaagaa ctctcttgga tccgggccac cgaacacgcg acgagcaaca atccgccaat
      901 ggatcttgcg gattggttcg agcaggagga gatggacagg gacatggcgg cccaagagtt
      961 gctagagaat gtcaggtacg gagtcgccta gacttcaaaa gtgggccgcc taccatgtct
     1021 ggtgcgcgga ttacagaccg ccacaccatc aaaaatgcat ctgcgtttgc ccacaccgcg
     1081 aatggttttt gttcataaat tccaatcccc ccatcggtcg tagggcgcga cagttcgcaa
     1141 ttgctgtagc cagttttgag gctagctttc tcaccaatcc tgagtcgtac atttatcccg
     1201 caatgaggac ttttcccgac gaccgcgttg cgaaatccat ttcagatcct agaaattgtt
     1261 tcggtttgat aagcccatct ctcatcgaac gaatcaaatc catgataagg gacaacccga
     1321 ctctgaaaga tgagcagaag aacattatta taaacgacgt acctataaga ccttaaattc
     1381 tttctgtcga taattcaact ttcgctttta caaataaaac atacttgttg tgctcgcctc
     1441 aaagaaatag gcaggcccaa cggacctgcc tctcatctca gacgctgtaa cccgcgccct
     1501 taccccatgt agctgacgaa cttgcccgtc atgtagacga gcagcccctc cgtgccgccc
     1561 tcgtagccga agccggagtc cttcacgccg ccgaaaggcg tctcaggcag cgcaaggccg
     1621 tggtggttga tggtgaccat gccgctgtcg agcgcatcag cgatctgcgt ggccgtcttg
     1681 ccgttacgcg tgaaggcata gctggcaaga ccatagggca gcttgttggc gcgtggcagc
     1741 acctcctcgg tgtccttgaa gcgcagcatg acagcgaccg ggccgaacgg ctcctcgttc
     1801 atgatgcgcg cgttgtcggg cacgtcggtc agaaccgtcg gctcgaagaa gttgccctgg
     1861 ttgccgatcc gcttgccgcc cgtggcgacc ttggcgccct tctcctgcgc atcctgcacg
     1921 aactgctcga tcgcgttcac acgccgcgga ttggcgagcg ggcccatctt gctctccgga
     1981 tcgaagccat cgcccacctt caggttcttg gcgatgtccg tgaacttggc gacgaacttg
     2041 tcgtagatct tctcctgcag gaagaagcgg gtcggcgaga tgcaaacctg gccggcgttg
     2101 cgatacttca tcgccgccat cagcttggcg acgtgctccg tatcgacgtc atcgtagacg
     2161 agcaccggcg catgcccgcc aagctccatg gtcgcccgct tcatatggct ggccgcgagc
     2221 gcgttgaggt gcttgccgac cgggaccgag ccggtgaacg agatcttgcg gatgatcggc
     2281 gacgggatca ggtactcgga gatctccgca ggggtgccat agacgaggtt gatgacgccc
     2341 gccggaacgc ctgcatcgtg gaagcagcgc acgaggccga tgggcgagcc tggcgtctcc
     2401 tcggggcact tgatgatgat ggagcagccg gccgccagcg ccgccgcgat cttgcgcacg
     2461 gcctgtgtca ccgggaagtt ccacggcgcg aagccggcca cggggccgat cggctccatg
     2521 atgaccatgt tgcgcacgcc atccgcgcgg gccgggatga tgcgcccata agcgcggcgg
     2581 ccttcctcgg cgaaccaatc cgtgatgtct gccgcgccca ggacctcgag gcgtgcctcg
     2641 atgaggatct tgccctgctc ctgcgtgagc actttggcga tatcgtccgc gcgtgcccgg
     2701 atcagatcgg ccgccttgcg caggatcttg gcgcgctcat aggccgagac gcgcttccat
     2761 tccttgaagc ccttgtccgc cgcagcgagc gccgcatcga gctcggcctt cttcgcgtgc
     2821 ggaagcttgc ccagaaccgt atccttcgag gggttcacga catccgacgt gacgccgctg
     2881 tcccccttcg tgaacttgcc gtcgatatag agttcgaggt tctcgtacat gggtgctctt
     2941 tccggtggtt ggtctggtag tcggagaagc ttcgcgaggg ggtgttcctc tcgtcaggca
     3001 tttgcctgat agcgtttagc aggaggccat agttgaggtc caacacggcg atattgatca
     3061 ggccattttg gctcagttcc gagccggtag gcagcgtgcc acgcccaatg cggatcgtcc
     3121 aggaatgcac gggcaatggc cacgaaatcc gcctggccgc tgcggatgat gccttcggcc
     3181 tgctcggcct cggtgatcat gccgacagcc atcaccggaa tgccgacggc cttcttgatc
     3241 tgctccgcga acggcacctg atagcccggg ccgacctgaa tgcgctgacc tgggtggttg
     3301 ccgcctgagg acacgtcgat gtaatcgcaa ccgaggtcct tgaggcgctt ggccagcgtg
     3361 atcgagtcct caatagtcca gccgccctct acccattcca cagccgagat acgcatgccg
     3421 agcggcttgc tctccggcca tgccgcccgc gtcgccttga acacttcgag cggaaagcgc
     3481 atccggttct cgaggctgcc gccccattcg tcatctcgct tgttcgacag tggcgacagg
     3541 aactgattca gcagataccc atgcgcgccg tgcagctcga tgagatcgag gccgatacgg
     3601 tccgcccggc gtgctgccgc tacgaaggct tcgatcagct cctcgatgcc ctgcggtgtc
     3661 agggcctgcg gcacgtgcca gccttcgttg aacgggatcg ccgacggagc tttggtctgc
     3721 catgccccct ctttcaacgg accgcccggc ggcgtgccct gccacggccg gtgggtcgac
     3781 gccttgcgcc ccgcatgccc gagctggatg ccgattttcg ccgatccgta cttgcggcag
     3841 gcatccacga cccgcttcag agcctgctcg ttctcgtccg agtagaggcc gagacaaccc
     3901 gggctgatgc gcccggctcg ctccacgccc gtcgcctcga tgatcagcag gcccgcgccg
     3961 gagttcgaca gcattccgag atgcatcatg tgccagtccg tcgccgagcc gtcctcggcc
     4021 gagtattggc acatcggcga aacgacgatc cggttgtcga gcttaaggtc gcgcagccgc
     4081 gccggggaga acagagcact gtctgacata ggccattcct ggataggggt ggaggagtga
     4141 tttcggaaaa agcgtagacc tcatagttca cgagatcaag acttccgaac catcgggtcg
     4201 gccagcgcgg aaatcgagcg ccctcgcggc ccgcctcgac tggaacgata gccggccatt
     4261 tcgccgcccg gcgccagctt ggactggggc ctcacgccgc tcttaccgtt cacggttcta
     4321 tacccatcag acgaccgatc ttagggttgc gttgatttgc acgtcattcc ggtcgcatag
     4381 ttggcgcaac cagcatacgg aaggctccta tcatgaccga gagcaccgag accagcctgc
     4441 acgggagatc ggacgggcgc gatcatgggc cggacgctgg cagtgcgacg gcatcgagtg
     4501 aatctacgca gctcggagcc atgggcaaag cgttcacaat tctcgaggct atcgccgccg
     4561 ccaagcaacc gatgaccatg tcggagctcg tgcgcgcctg tcgccttacc aaacccacgg
     4621 cacatcgcat cacgacgatg ctggtcgaga tgggctatct cgagaaggag ccgctgaagc
     4681 gtggcctgat cgaaggaccg cggctgattt cgctggctct gacgacgctc gccgcagccg
     4741 ccccccgcag ccgccgccat gccattctcc ggtcgatctc cgaacagacg ggggagacct
     4801 gcaatttcgg tgtcctggcc ggctcggagg tcatctatct cgatcgggtg gaggccaagt
     4861 ggccactggc gctcaggttc gaggcgggca gccgcgtgcc cgcacactgc acggcg
//
LOCUS       DASVDU010000279         9635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_840547, whole genome shotgun sequence.
ACCESSION   DASVDU010000279 DASVDU010000000
VERSION     DASVDU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840547"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..151
                     /locus_tag="VF226_18615"
     CDS             14..151
                     /locus_tag="VF226_18615"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7076056.1"
                     /translation="MRSSVGIGLLWNTPLGPLRLDYAYALTKEDYDETQSIRFGASTK
                     F"
     gene            complement(427..1104)
                     /locus_tag="VF226_18620"
     CDS             complement(427..1104)
                     /locus_tag="VF226_18620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7076057.1"
                     /translation="MRDLTLVFDLDGTLIDTAPDLIRATNHVLANRGLEPLPPERLLS
                     EISFGARHMIATGLALRGISYSDSELDELFEAFQVYYEQNIAVESRPFEGLTDLLQDC
                     LSEGAKLAICTNKLESLSRQLLDQLGLTRLFRALAGRDTFPVCKPHPDHLLGAIRLAG
                     GNPAHAVMVGDSEVDISTAKAARVPVIGVTFGYTRIPVTELGADAIIDSYAEFRAALD
                     RVLPRPS"
     gene            1270..2712
                     /locus_tag="VF226_18625"
     CDS             1270..2712
                     /locus_tag="VF226_18625"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7076058.1"
                     /translation="MDKPVISLLRTQCYVDGRWVGEPSLPVIDPATGEEIAKVPMMSA
                     EDTRAAIDAAARAQPEWAGMLAKERSKILRRWYELQLEHADELALLMTREQGKPLAEA
                     KGEVMYAASFVEFYAEEAKRVYGETIPTFKKDARIVCIKQPVGVVAAITPWNFPAAMI
                     TRKVAPALAVGCTVVCKPASETPLTALALAELAERAGVPKGVLNMVTGKASVIGGELT
                     SNPTVRMLTFTGSTEIGKILIEQCAKTVKKVGMELGGNAPFIVFDDADLDKAVQGAMA
                     SKYRNAGQTCVCANRIYVQDGVYDAFAQKLAAEVAKLKVGRGTEAGVTTGPLINKAGV
                     EKVEEHIADATAKGAKVILGGKRHSLGGNFFEPTVLTEVTTDMKVAREETFGPVAPLF
                     RFKTDEEAIKLANDTEFGLAAYFYSRDIGRVWRVGEGLEYGIIGINEGIISSELAPFG
                     GVKESGLGREGSHHGIEEFLEIKYMLMGGV"
     gene            2712..3419
                     /gene="rpiA"
                     /locus_tag="VF226_18630"
     CDS             2712..3419
                     /gene="rpiA"
                     /locus_tag="VF226_18630"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX7076059.1"
                     /translation="MSDALKLKAAEKAIEFVQPGMKLGLGTGSTAAKFIDLLGARVKE
                     GLDVIGVPTSEATRMQAAALGIRLTTLDDDPELDLTIDGADEIDDALQLIKGGGGALL
                     REKIVAAASNRMIAIADSSKRVPVLGAYPLPVEVVRFGFGATRRMIEKVAADVGCTGH
                     ILLRMGLDGQPFVTDGGNYIFDCAFGRIEDANALDEALRLIPGVIEHGLFLDLADAAI
                     IAGPDGVEVLEAENKRD"
     gene            3586..4968
                     /gene="gor"
                     /locus_tag="VF226_18635"
     CDS             3586..4968
                     /gene="gor"
                     /locus_tag="VF226_18635"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX7076060.1"
                     /translation="MTKYDFDLFVIGGGSGGVRAARIAAGHGARVALAEGFRLGGTCV
                     IRGCVPKKLLVYASRFADEFEDAAGFGWSVGETQFDWARLIAAKDREIARLENIYRNN
                     LEKAGVTIFEEWAEVASPNSVRLVREDRIVTARYILVATGSSTDIDTRVIGSEEVITS
                     NDAFELKELPKRIAIAGGGYIAVEFAGIFAGLGAETTLVYRGEKLLRGFDDDLRDGLM
                     EEYAKRGIRVLTNATFWQIERTEKGLVGSLSDGTVLEADQIMFAIGRSPNTAALGLKK
                     VGVALGERGEIIVDADNRSNVPSIYAIGDVTNRVNLTPVAIREGHAFADTVFGDKPWR
                     VDYDNIPTAVFSTPEIGTVGLTELDAISAGYTIDIYKSRFRPMKHTLSGRDERTIMKL
                     VVDRPTQKVLGVHMLGPDAAEIAQMAAIALRLGATKADFDATMPLHPSAAEELVTMRE
                     KWVPQRAAAE"
     gene            5103..5828
                     /locus_tag="VF226_18640"
     CDS             5103..5828
                     /locus_tag="VF226_18640"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7076061.1"
                     /translation="MTANAIARASAALLFCLAALASGQARAQNYDGAGLLRFGVYGQV
                     ANTNFDIERPIVARGSASDTAGGGGATFGYDYLFQSGLILGIESDIGFDTLQTEHRGR
                     EFSTNFMASVRGRLGGYVSPNLLLYGTAGVAFLGVEYDGLTSPLTGLQFGDSGTVTGW
                     TAGAGAEYDWNGIVFFGEYLFADYGTFSSDEVVESLDETGLFVIETPFRNEIDIDQHL
                     FRFGVKFIIGHDYRVGEVYAPLK"
     gene            5912..7147
                     /locus_tag="VF226_18645"
     CDS             5912..7147
                     /locus_tag="VF226_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7076062.1"
                     /translation="MRQVTVIGAGIIGMCVADYLQRLGASVTVLDPIEPGRSCSFGNA
                     GALSPGSCVPVAMPGVLKQVPKWLTDPSAPLVLRWSYLPWALPWLTRFLRASSKKQVE
                     RSASALSALLRPVFENYAPLLEEANAADLIRRQGQLYVYETEAAYRGDAFGWELRRRH
                     GARIEVLSADELRQHEPALAPFFTKGVLLPEHGYCLDPFGLVQALADSVRRKGGTILG
                     QKVLDVEMDADRPRALVTAAGRHQVDAVVVAAGAWSHQLARMFGHKVPLETQRGYHAM
                     LRDPSVSPRTSIMWAEKKFLATPMRMGLRFAGTVELAGLHAPPDYRRAATLLEFGKRM
                     FPGLTGGEVSRWMGHRPCLPDSVPVIGPSTKVRNVYFAFGHGHLGLSCASTTGRLVAE
                     LVMGAKPCIDPEPYRIDRF"
     gene            complement(7171..7956)
                     /locus_tag="VF226_18650"
     CDS             complement(7171..7956)
                     /locus_tag="VF226_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX7076063.1"
                     /translation="MTDKMLARRDGHIGTLIFNNPARHNAVSFDMWEAAERILDDFEA
                     DPEVRVIVLTGAGEKAFVSGADISKFEDERAAEDAVRRYNAQVERIYARIYTMPKPTI
                     AKIRGYCIGGGLNLAISCDLRFATRGSLFALPAARLGLGYGYPGLRRYIQTLGPAVTK
                     EIFFTARQFSADEALAWGVVNRVVGDEELDEVVRTTAETIAQNAPLTIRCIKLAATEA
                     LRDPEDRDLDACEAAVQACFNSEDYVEGRRAFLEKRKPVFQGK"
     gene            complement(7966..9207)
                     /locus_tag="VF226_18655"
     CDS             complement(7966..9207)
                     /locus_tag="VF226_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX7076064.1"
                     /translation="MHLEHPHEPPAGIRHLLADLSASNVSNALVGFVFSATGPVAIIL
                     AIGTRGGLSEAQIASWLFGAYFLNSIITIAFSLAYRQPLVFFWTIPGGVLVGPALAHL
                     TFPEVVGAFVVTGIGTAILGMTGLVRRVMQAVPMPIVMSMVAGVFLQFGLDWVRAFDQ
                     AFWISAPMTVAYLMLTRIPRFSRMVPPLIGALAVGIFISAMTGEFAPAIGTVTAVAAP
                     QIVWPEFSWQAIAELSIPLAITVLVVQNGQGVAVLTAAGHKPPVNSIAAISGVGGFVT
                     AFLGAVSTCLTGPVSAILTTAGERERQYAGAVVVAVLAMAFGLFSPLFTRLLLAAPAA
                     FIATLAGLAMLRVLQASFATAFEGRFGFGALVTFLITVSGISVANIGAPFWGLVFGYI
                     ASQLLEPSDFRIQPAPDIQGK"
     gene            complement(9243..>9635)
                     /locus_tag="VF226_18660"
     CDS             complement(9243..>9635)
                     /locus_tag="VF226_18660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7076065.1"
                     /translation="GPELLLSAQAAQNTSLAIHELATNASKYGALSQPNGHVTIEWRI
                     EGEATEPMLVFEWIERGGPPATPPSRQGFGHTLLQTVLSDAEFKPEIRFEPDGLHYRA
                     TVRLSAVLADSSQVIPQSLSGVGPSGKH"
BASE COUNT         1726 a         2954 c         3280 g         1675 t
ORIGIN      
        1 cgacgacgcc agcatccggt cttctgtcgg tataggtctg ctctggaaca cgccgctggg
       61 tccgttgcgc ctcgactacg cctacgctct gacgaaagag gactacgacg agacgcagtc
      121 gatccgcttc ggcgcttcca cgaagttctg acgctgacgc ggccggggct caatgctgag
      181 cctcggctgc acttgcgatg cggtcagggg ttaacccgac gcgctatcgc taaaggtctt
      241 ccgaatagct agtaccggtc gcagcgattg cttcttcacc tctcccatgg gggagaggtc
      301 ggcccgcagg gccgggtgag gggctgcaac agacgccact tgcggaacct cctctccccg
      361 ccggggagag ggagatgggg cgcgagcttg gtcgccgtcc cgcctgcgcg aaattgccct
      421 cgtgcgctaa cttggccgcg gcagaactcg gtccagcgct gcgcggaatt cggcatagct
      481 gtcgatgatg gcgtccgcgc caagctccgt cacgggaatg cgcgtgtagc cgaacgtgac
      541 cccgatgacc ggaacgcggg ctgcttttgc cgtgctgata tcgacctcgc tgtcgcccac
      601 catcacggca tgcgccggat tgccgccggc aagccggatt gcaccgagca ggtggtcggg
      661 atggggcttg cagacgggga aggtgtcgcg gccggcgagg gcgcggaaca ggcgcgtcag
      721 gccaagctga tccagaagct gccgggagag gctttccagt ttgttggtgc aaatggcaag
      781 cttcgcgccc tcgctgaggc aatcctgcag aaggtcggtc agtccctcga acgggcggct
      841 ttcgacggca atgttctgtt cgtagtagac ctggaacgcc tcgaacagct cgtcgagctc
      901 gctatccgaa taggagatac cgcggagcgc caggcctgtc gcgatcatgt gccgcgcgcc
      961 gaagctgatc tcggacagca gccgctccgg tggcagcggc tcgaggcccc ggttcgccag
     1021 cacgtgattt gtcgcacgga tcaggtcggg tgccgtgtcg atgagcgttc cgtcgaggtc
     1081 gaagacgagg gtcaggtcgc gcatgatcat ccgtcagtgg tgtgcgttcc ggtaaggctt
     1141 aggccatccc gccggccctg tcaccgctga cgaccatgct tgccagcgcc ttttcccgga
     1201 cttagatcaa ggcagccaga acggctgagg ctgtagtggc ctggttttcc ccgaagaagg
     1261 agtgctccca tggacaagcc cgtcataagt cttctcagga cgcaatgtta tgtcgacggc
     1321 cgctgggttg gtgagccgtc ccttcccgtg atcgatcccg cgacgggcga ggagattgcc
     1381 aaggttccca tgatgagcgc agaggatacg cgcgcggcga tcgacgcggc ggcccgtgcg
     1441 cagcctgagt gggccggcat gctggccaag gagcgttcga agatcctgcg tcgctggtac
     1501 gagctgcagc tcgagcatgc cgacgagctg gcgctgctca tgaccaggga gcagggcaag
     1561 ccgctcgccg aggcaaaagg cgaggtgatg tatgcggctt cgttcgtgga gttctacgcc
     1621 gaggaagcca aacgcgttta cggcgagacc atcccgacct tcaagaagga cgcacgcatc
     1681 gtctgcatca agcagcccgt cggcgtggtc gcggccatca ccccgtggaa cttccctgct
     1741 gcgatgatca cccgcaaggt ggccccggct ctggccgtcg gctgtacggt ggtctgcaag
     1801 ccggcctccg aaacgccgct gaccgcgctc gcgctggccg agctggcgga gcgtgccggc
     1861 gtgccgaagg gcgtgctcaa catggtcacc ggcaaggcga gcgtgatcgg cggcgagctg
     1921 acctcgaatc cgaccgttcg gatgctgacc ttcaccggct cgactgagat cggcaagatc
     1981 ctcatcgagc agtgcgccaa gaccgtgaag aaggtcggca tggagctcgg cggcaacgcg
     2041 cccttcatcg tgttcgacga tgccgatctc gacaaggccg tgcagggggc gatggcctcc
     2101 aagtaccgca atgccggcca gacctgcgtg tgcgccaacc gcatttatgt ccaggatggc
     2161 gtctatgacg cgttcgcgca aaagctggcg gccgaggtgg ccaagctcaa agtgggccgc
     2221 ggcaccgagg cgggcgtgac gacgggcccg ctcatcaaca aggccggcgt cgagaaggtc
     2281 gaggagcaca tcgccgatgc gacggcaaag ggcgccaagg tgatcctcgg cggcaagcgt
     2341 cactcgctcg gcggcaactt cttcgagccg acggtgctga cggaggtcac caccgacatg
     2401 aaggtggcgc gagaggagac gttcggacca gtggcgccgc tattccgctt caagacggac
     2461 gaggaggcga tcaagctcgc caatgacacc gagttcggtc tcgcggccta cttctactct
     2521 cgtgacatcg gccgcgtgtg gcgcgtcggc gaggggctcg agtacggcat catcggcatc
     2581 aacgagggca tcatctcgtc cgagttggct cccttcggcg gcgtgaagga atcgggcctc
     2641 ggccgcgagg ggtcgcatca cggcatcgag gagttcctgg agatcaagta catgctgatg
     2701 ggcggcgtct gatgtcggac gccctgaagc tgaaggcggc ggagaaagcg atcgagttcg
     2761 tccagcccgg catgaagctc gggctcggca cgggctcgac cgctgccaag ttcatcgatc
     2821 tgctcggggc cagggtgaag gagggcctcg acgtgatcgg ggtccccacg tctgaggcga
     2881 cgcggatgca ggcggcggcg ctcggcatcc ggctgacgac gctcgacgac gaccccgagc
     2941 tcgatctgac catcgacggc gcggacgaga tcgatgacgc cttgcagctc atcaaaggcg
     3001 gcggcggcgc gctgctgcgc gaaaagatcg ttgcggccgc gtccaaccgc atgatcgcga
     3061 ttgcggattc ctccaagcgc gtgccggtgc tcggtgccta tcccttgccc gtggaggtcg
     3121 tccgtttcgg tttcggtgcg acccgcagaa tgatcgagaa ggtggccgcc gacgtcggct
     3181 gcacgggcca tattcttttg cgcatggggt tggatggcca gccgttcgtg acggatggcg
     3241 gcaactacat ttttgactgt gctttcggcc gcattgagga tgccaatgcg ctggacgagg
     3301 ctttgaggct catccccggg gtcatcgagc acggactttt cctcgacctt gccgatgccg
     3361 ccatcatcgc cgggccggac ggcgtagagg tcctcgaggc ggagaacaag cgcgattgaa
     3421 gactgtccct aactttggat cggtggagtg gatcggtgcg cggccaagct tccacctgcc
     3481 gtcatctcgg tgcttgtcac cgggatccac cttacgactt gctccggcgc acgcgaatgt
     3541 ttggatccca agcacaaggc ccggaatgtc tcagcagggc aagatatgac caagtatgac
     3601 ttcgacctgt tcgtgatcgg cggcggctcg ggtggggtgc gggcggcgcg gatcgcggcg
     3661 ggccatggtg cgcgcgtggc tctcgcggag gggttccggc tcggcggaac gtgcgtcatc
     3721 cgcggctgcg tgccgaaaaa gctgctggtc tatgccagcc ggtttgcgga cgagttcgag
     3781 gatgcggccg gtttcggctg gtcggtgggc gagacgcagt tcgactgggc tcggctcatc
     3841 gccgccaagg atcgcgagat cgcccggctg gagaacatct accggaacaa cctggagaag
     3901 gccggtgtca ccatcttcga ggagtgggcg gaggttgcga gcccgaactc ggtccgcctc
     3961 gtcagggagg accgcatcgt cacggcccgg tacattctgg tcgcgaccgg gagcagcacg
     4021 gacatcgaca cgcgcgtcat cggctccgaa gaggtcatca cgtcgaatga cgcattcgag
     4081 ctgaaagagc ttccgaagcg aatcgctatc gccggcgggg gctatatcgc ggtcgagttc
     4141 gccggcattt tcgcgggact gggcgcggag accacattgg tttatcgcgg cgagaagctg
     4201 ctgcgcggct tcgatgacga tctgcgcgac ggcctcatgg aggagtacgc caagcgcggt
     4261 atccgcgtgc tgaccaacgc caccttctgg cagatcgaac ggacggagaa ggggctcgtc
     4321 ggcagtctca gcgatgggac cgtgctcgag gccgaccaga tcatgttcgc catcggccgg
     4381 agtccgaaca cggcagcgct tgggttgaaa aaggtcggcg tggccttggg tgagcgcggg
     4441 gagatcatag tggatgccga caaccgttca aacgtcccca gcatctacgc catcggcgac
     4501 gtcaccaacc gcgtgaacct gacgcctgtc gcaatccgcg aaggccatgc gttcgccgac
     4561 accgtgttcg gcgacaagcc atggcgggtc gactacgaca acatcccgac ggccgtcttc
     4621 tccaccccgg aaatcggaac ggtcggcctg accgagctgg atgccatctc cgccggctac
     4681 acgatcgata tctacaagtc gcgcttccgg ccgatgaagc acacgctgtc gggacgtgac
     4741 gagcgcacca tcatgaaact cgtcgtcgat cggccgacgc aaaaggtgct gggtgtgcac
     4801 atgctgggcc ccgacgcggc ggagatcgcc cagatggcag ccatcgcgct gcggctcggt
     4861 gcgacgaagg ccgatttcga cgcgaccatg ccccttcacc ccagtgctgc cgaggagctg
     4921 gtcacgatgc gggagaaatg ggtgccgcaa cgggccgccg ccgagtagct ggagccacac
     4981 cgactttggt ttttgcggcc tggttaacag caggtaaatg ctcattacca caacctgtgg
     5041 tagtgtttgc tgacgagtcg cctgcgcgtt ccctgcacca gcgcacatga atgaggacgg
     5101 ccatgacggc aaacgccatc gcccgtgcct cggctgcact cctgttctgc ttggctgcct
     5161 tggcctcggg acaagctcga gcacagaatt atgacggcgc cggcctgctg aggttcggag
     5221 tctacggcca agtcgcgaac accaacttcg acatcgagcg gcccatcgtg gcaagaggct
     5281 cggcgtcgga tactgccggc ggcggcggcg cgacgttcgg gtacgattac ctgttccaaa
     5341 gcggactgat cctcgggatc gaatccgata tcggtttcga tacgcttcag acggagcatc
     5401 gcggacggga gttcagcacc aacttcatgg cgagtgtccg tggccgtctc ggcggctatg
     5461 ttagccccaa cctgctgctc tacggcacag cgggcgtcgc atttctcggt gtcgagtatg
     5521 atgggctgac aagcccgctg acggggctgc aattcggcga cagtggcacc gtcacgggct
     5581 ggacggcggg tgcgggcgct gaatacgact ggaacggaat cgtcttcttc ggtgagtacc
     5641 tgttcgccga ttacgggacg ttctcgtccg atgaagtcgt cgagagcctc gacgagacgg
     5701 ggctgttcgt gatcgagacg cctttccgga atgagatcga catcgaccag cacttgttcc
     5761 ggttcggcgt caagttcatc atcggccacg actaccgcgt cggcgaggtc tacgctccgc
     5821 tgaagtagct gacaatccag ccgggatcgg tcaagctggc attcgcaggc gggaccggcg
     5881 tccggctgcg agggttgcga agcagaagtc tatgcgtcaa gtcactgtca tcggggccgg
     5941 catcatcggc atgtgcgttg cagactacct gcagcgcctt ggcgcgagcg tcacggttct
     6001 cgatcccatc gagccgggac gaagctgctc ctttgggaat gccggcgcgc tcagccccgg
     6061 gtcctgcgtg ccggtggcca tgccgggtgt gctgaagcag gtgccgaagt ggctcacgga
     6121 tccatcggcg ccgcttgttc tgcgctggtc ctatctgccc tgggcgctgc cgtggctgac
     6181 acggtttctg cgggcgagca gcaagaagca ggtcgagcgc agcgcgtctg ctctgagcgc
     6241 cttgctgcgt ccggtgttcg agaattacgc gccgttgctc gaggaagcca acgccgcgga
     6301 tctcatccgg cggcagggcc agctctacgt ctacgagacc gaggcggcct atcggggcga
     6361 tgcgttcggt tgggagctgc gccggcgtca tggcgcccgc atcgaggtgc tctcggctga
     6421 cgagctccgg caacacgagc cggcactggc gccatttttt acgaaaggcg tcctccttcc
     6481 cgagcacggc tattgcctcg atccgttcgg gctggtccag gcgctggccg acagcgtgcg
     6541 gcgcaagggc gggaccatcc tcggccagaa ggtgctcgat gtcgaaatgg atgccgatcg
     6601 cccgcgggcg ctcgttacgg cggcgggccg ccatcaggtg gatgcagtcg tcgtcgctgc
     6661 cggcgcctgg tcgcaccagc tcgcacgaat gttcgggcac aaggtcccgc tcgagaccca
     6721 gcgcggctat cacgccatgc tgcgcgaccc gagcgtgagc ccacggacca gcatcatgtg
     6781 ggcggaaaag aagtttctgg cgacgccgat gcgaatgggc ctgaggttcg caggcacggt
     6841 cgagctggcg ggccttcacg ccccgcccga ttaccgccgc gccgccacgc tgctggagtt
     6901 cggcaagcgc atgttccctg gtctgaccgg tggcgaagtg agccgctgga tgggccatcg
     6961 gccctgcctt ccggattcgg tgccggtgat cggcccctcg acgaaggtga ggaatgtcta
     7021 tttcgccttc ggccatggcc atctcgggct gtcgtgcgct tcgacgaccg gacggctggt
     7081 ggccgagctg gtcatggggg caaagccctg catcgatccg gagccttatc ggatagacag
     7141 gttctgaagg cccggggagc ctggcaccca tcatttgccc tggaagacgg gcttgcgctt
     7201 ctccaggaat gcgcggcggc cttccacgta gtcctcggaa ttgaagcagg cctgcacagc
     7261 cgcttcgcac gcgtcgaggt cgcggtcctc cggatcacgc agcgcttccg ttgccgcgag
     7321 cttgatgcag cggatcgtca gcggcgcatt ctgcgcaatg gtttccgccg tggtccgcac
     7381 gacctcgtcg agctcttcgt cgccgaccac gcgattgacg acgccccagg ccaatgcctc
     7441 gtcggcactg aactgccgcg cggtgaagaa gatctccttg gtgaccgccg ggccgagggt
     7501 ctggatgtag cgcctgagcc cgggatagcc gtagccgagc ccaagtcgcg ccgccgggag
     7561 ggcaaagagg gagccacgcg tcgcaaacct gagatcgcaa gagatggcga ggttgagacc
     7621 gcccccgatg cagtagccgc ggattttcgc gatggtcggc ttcggcatcg tgtagatgcg
     7681 cgcgtagatg cgctcgacct gggcattgta gcgccggacc gcatcttccg cggcgcgctc
     7741 gtcctcgaac ttggaaatgt cggcgccaga gacgaaggcc ttttcgcccg cgccggtcag
     7801 gacgatgacg cgcacctcgg ggtccgcctc gaagtcgtcg aggattcgtt cggccgcctc
     7861 ccacatgtcg aacgagacgg cgttgtggcg ggccggattg ttgaagatca gggtgccgat
     7921 gtgcccgtcg cgccgggcca gcatcttgtc ggtcatgagc gtgctctatt tcccctgaat
     7981 gtcaggcgct ggctgtatgc ggaaatcgga cggctccagc agctgggagg cgatgtagcc
     8041 gaagacgagg ccccagaacg gggcgccgat gttggccacg gatataccgc tcacggttat
     8101 caggaacgtg accaacgcgc cgaagccgaa gcgaccctcg aaagcggttg cgaacgaagc
     8161 ctgcaatacg cgcagcatgg caaggccggc gagggtggcg atgaaggctg ccggggccgc
     8221 cagcagcagc cgcgtgaaca ggggagagaa gaggccgaag gccatagcca gcacggcgac
     8281 cacgacagcg ccggcgtact ggcgctcacg ttcgccggcg gtcgtcagga tcgcgctgac
     8341 cggtccggtc aggcaggttg agaccgctcc caagaaggcg gtgacgaagc cgcccacccc
     8401 ggagatggcg gcgatgctgt tgaccggcgg cttatggccg gcggccgtca aaacggcaac
     8461 gccctggccg ttctggacga cgagcaccgt gatcgcgagt ggaatcgaga gctccgcgat
     8521 ggcctgccag gaaaactcgg gccatacgat ctgcggtgcc gcgacggcgg tgacagtgcc
     8581 gatggccggc gcaaactcgc ccgtcatggc cgagatgaaa atgccgacag ccagtgctcc
     8641 gatgagtggc ggcaccatcc gcgaaaatcg ggggatgcga gtgagcatca ggtaagccac
     8701 cgtcatcggt gccgagatcc agaatgcctg atcgaacgcc cgcacccagt cgaggccgaa
     8761 ttgcaggaaa acaccggcca ccatgctcat gacgatcggc atgggcaccg cctgcatgac
     8821 ccggcggacg aggcccgtca tgccgaggat cgccgtgccg attccggtga cgacgaaggc
     8881 gccgacaacc tcgggaaagg tcagatgcgc cagtgcgggc ccgacgagca cgccgcccgg
     8941 tatggtccag aaaaatacga gcggctgacg ataggcgaga ctgaaagcga tggtgatgat
     9001 gctgttgagg aagtacgcgc cgaacagcca tgacgcgatc tgcgcttcgc tcaggccgcc
     9061 ccgggttccg atggccagga tgatcgcgac cgggccggtt gccgagaaga cgaacccgac
     9121 cagggcattg ctgacgttcg acgccgaaag atccgcgagc agatggcgga tgccggccgg
     9181 gggctcatgg ggatgctcga ggtgcatgag gcctactgct caacggaaag gaagatcagc
     9241 ggtcaatgtt tcccagaggg gccgacaccc gaaaggctct gcggaatgac ctggctgctg
     9301 tctgccagaa ctgccgaaag gcggacggtt gcgcgataat ggaggccatc cggctcgaac
     9361 ctgatttccg gcttgaactc ggcatcgctc agcacggtct ggagcagggt gtgaccaaat
     9421 ccctgccgcg agggaggggt ggctgggggc ccgccgcgct cgatccactc gaacaccagc
     9481 atcggctcgg tcgcctcgcc ctcgatgcgc cattcgatcg tgacgtggcc gttcggctgg
     9541 gacagcgccc cgtatttgga cgcattggtc gccagctcgt ggatcgcgag agacgtattc
     9601 tgcgccgcct gcgcggacag cagcagctcg ggtcc
//
LOCUS       DASVDU010000280        23747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_904842, whole genome shotgun sequence.
ACCESSION   DASVDU010000280 DASVDU010000000
VERSION     DASVDU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23747
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904842"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(811..1323)
                     /locus_tag="VF226_18665"
     CDS             complement(811..1323)
                     /locus_tag="VF226_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076066.1"
                     /translation="MSIANPAVCDIPRRPARLTIVAARTVAGATTRPGVRAYSFLTAC
                     RARSSCEGLVPSRQIREEGGEYDIPDPTEDEHYEPRSEAFDAADAHTEEQREAMALLD
                     DAENLLAVLMAAIEDDGDSRAMQTEAVLKVVKEKLREAHTRIDRQESQYRNLFLAYFE
                     LKAESEREVE"
     gene            1543..1821
                     /locus_tag="VF226_18670"
     CDS             1543..1821
                     /locus_tag="VF226_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076067.1"
                     /translation="MAPSAVRYRSEAEGLAQAVARTRPRVCFFGHHHTRLDAEVAGVR
                     CIGLNKVAMPGNLVALGAGPRLRDLGRMVASPGHRFSNAFVQRSRETR"
     gene            1818..2921
                     /locus_tag="VF226_18675"
     CDS             1818..2921
                     /locus_tag="VF226_18675"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7076068.1"
                     /translation="MTVLTGPRQCGKTTLARELLSEDSPNYFDLEDPVSLTRLEEPRT
                     ALELLGGLIVIDEVQRRPDLFPVLRVLADRRQRKTKFLILGSASGELLRQSAESLAGR
                     MERIEISGFTLAELGADAARKLWRRGGFPRAYLAANERDSMTWRKQFIQTLLERDFPQ
                     WGVRVPAAALLPFWTMLAHYHGQIWNASEPARALGVNPSTTRRYLNLLTDAMMLRQLK
                     PWYANLGKRQVKSPKVYVRDSGLLHQLLGIDSEKALLSHPKIGASWEGFVIEQVLASE
                     PHDEAWFWATHQGAEIDLLLRRGEKLLGIECKRADAPRLTPSIRTAIEDLGLERVAIV
                     YPGSKRFALHDRVEVVPLASLAEPGQLFVPAAA"
     gene            2989..4116
                     /locus_tag="VF226_18680"
     CDS             2989..4116
                     /locus_tag="VF226_18680"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7076069.1"
                     /translation="MTENFPSSMHLGTDSYSPMRKEPLHQNEGTSASIRSGRSLIGAT
                     MPFTVESVTRSWWHVGSTFVMLVGSLAGAALAPAWPVRLILSVLAALLMVRAFITYHD
                     YMHGSILARSRLAWILFRIYSVLFLTPPRSWKTSHNYHHGHVGQTSAATDGAFPIMTT
                     TTWRSASWATRARYRAVRHPLTVLTGYLTIFFFSLTLMPLFRNPARHWGALPVLLAHG
                     ALIATLWMLGGLSMAFYVVLLPMTMASALGSYLFFAQHSFKRMHIMPSETWTYYRAAM
                     ESSSYMRLNKLMQWFTGNIGFHHIHHLNVRIPFYRLAEAMDAIPELQSPMTTSLTPRD
                     IAGCFRSCLWDEDRQRMVSYREASPSGGLCESARRSSGCPG"
     gene            complement(4342..4611)
                     /locus_tag="VF226_18685"
     CDS             complement(4342..4611)
                     /locus_tag="VF226_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7076070.1"
                     /translation="MNIILWLVAGGLIGWLASMFTGTDGLQGIILNVVVGIVGAALGG
                     WLLGGLVGAGSINQGDFSVPGLFVSFLGAVILLIFFKFARGGALR"
     gene            complement(5099..5233)
                     /locus_tag="VF226_18690"
     CDS             complement(5099..5233)
                     /locus_tag="VF226_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076071.1"
                     /translation="MGIGLIGLIIVVLIVAALVKSIAIGGIGGLLLLILLILLLTGRL
                     "
     gene            complement(5235..6242)
                     /locus_tag="VF226_18695"
     CDS             complement(5235..6242)
                     /locus_tag="VF226_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076072.1"
                     /translation="MATIHEETLVADTERRPLISWGAVLAGLAFVVAATWLLFMLGSA
                     IGLSVADATDTEAMGKGLGIGATVWMLVTAFLAYFLGSLLTARLAGKSERAIGMLHGV
                     TLWSVGTALMLVLGAWGIRGLIQTGTSVVSTAASAGASIGGAGAEAGGGEAGRLADSP
                     LMTTVQARIKREIAAALTEAQRPDAAEPAPARDTPATAAAPSTAAPPAASQQEIRRAL
                     DEIDADTLMAAARPILAGEPERAKDVLAVNTSLSEREIDAVVDGVSQEMSQQIEEAKQ
                     QMDQAVETASTYAQAVTWAAFIAGALSLIVAIAGGWLGARTVHRLYAVDVSRRTTAGR
                     G"
     gene            complement(6395..6676)
                     /locus_tag="VF226_18700"
     CDS             complement(6395..6676)
                     /locus_tag="VF226_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076073.1"
                     /translation="MEHGAPHVLRSVRWSTQRYIHYSEYLMHTVDDGRAIESLLELAH
                     EPCPARLVKQDRVTASASVSGDMKSASSVCRSLRMRSFMVVRPASPAEA"
     gene            6815..7027
                     /locus_tag="VF226_18705"
     CDS             6815..7027
                     /locus_tag="VF226_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076074.1"
                     /translation="MNIAKLLLLLTVASFTVTACDMDDSAAENTGEAIDEVGEKIDDA
                     ATDAGNAIEDACEEVKESADAEDTDC"
     gene            7281..7709
                     /locus_tag="VF226_18710"
     CDS             7281..7709
                     /locus_tag="VF226_18710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076075.1"
                     /translation="MFRVKQLIVGAALLATAAGCASIRGTDTIADTTRTGAIHDVSFE
                     ERMTPVNLRVQPGDEVRWVNKRSTPVTVEFLGDALADVTCQSGFSSLLRRQQETATIE
                     PNESASLCFGRAGTVTYNARMESPVAGGQMIESGTIRVGQ"
     gene            complement(7992..8663)
                     /locus_tag="VF226_18715"
     CDS             complement(7992..8663)
                     /locus_tag="VF226_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076076.1"
                     /translation="MQAGLYRTICLSAARFDGFFGNARFHDEYQATHGSLAVGALWDE
                     RDHWDPSVRFRVHVRLPQLSERFNAFVGRLDPDEYVTELRDDFDTLPRQFARRDDDAV
                     LFGLGYRRPGRMGGHFDADIGAKLRWPLEPYVKGTYRLGVPFGDQNLLRLDETIFWRR
                     GEGFGATTRFDLERLLAEDFLVRWTGSGTFTGESEGVRWFSSLTLYQNLGGGHALAYE
                     AGISG"
     gene            complement(8983..9690)
                     /locus_tag="VF226_18720"
     CDS             complement(8983..9690)
                     /locus_tag="VF226_18720"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7076077.1"
                     /translation="MGTEERVPMVSYRNPRQRRYTPGTRRPLARLWRTFLTGLVAIIP
                     LGVTAYVVYWLFTAADSLFSGIGQALLPPDWYFPGLGLIGAVLAVLALGAVLNAWVFG
                     RVMLTLGTRLLERIPLVKTVYSGVRDLMLFVSRAPDDDRRHVVLVTLPGDIRLIGFIT
                     DTAPAQAVPELTLEDEPLLAVYLPMSYQIGGYALYLPERCLQRLEMSMEEGMRMVLTA
                     GMNRPRTHKPADAKRMT"
     gene            complement(9805..10239)
                     /locus_tag="VF226_18725"
     CDS             complement(9805..10239)
                     /locus_tag="VF226_18725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7076078.1"
                     /translation="MARATRQRRQVVLLFLDLNGFKAINDSLGHVEADRLLQQVGGRL
                     TSCIRRSDTACRFGGDEFVVLLTGIERRNQAMAVINTVIVELAAPYQIDGIPVSMTAS
                     LGVATFPADGQNYHELLQAADATMYRDKASCCTVSALGPEAV"
     gene            10253..10420
                     /locus_tag="VF226_18730"
     CDS             10253..10420
                     /locus_tag="VF226_18730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076079.1"
                     /translation="MQQAAVRQAGQLVVIGNATRPDQSFCKPCHFLPERPILSQQCVE
                     QADERFEALHE"
     gene            10784..11035
                     /locus_tag="VF226_18735"
     CDS             10784..11035
                     /locus_tag="VF226_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076080.1"
                     /translation="MNQSARTAKQIGAIVRRARRNVGLTQADLGKRVGLRQATISRLE
                     KGEEETKLGTLLDVLSALGLEITIARRGKTSARNVEDIF"
     gene            11035..12351
                     /locus_tag="VF226_18740"
     CDS             11035..12351
                     /locus_tag="VF226_18740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEX7076081.1"
                     /translation="MARKRTRIPLNVYINGRLVGRLRRETSGAIDFHYDESWLTWEHT
                     FPVSLSLPLREDRYVGDPVIAVFDNLLPDSEDIRRRVAARSDADGIDPYSLLSAIGRD
                     CVGALRFLPDGKDPGKADEIDARTIGYEEIADRLANLANAPLGMGDDAEFRISIAGAQ
                     DKTALLYWQDGWHVPHGGTATTHILKPQIGQRGNYDLSHSVENEHMCLEIMRALGLPA
                     ARTEIADFAGTRALVIERFDREWTKDGRLLRVPQEDCCQALSVPPTRKYQSDGGPGMV
                     EVFALLKASDDPAHDQLVFMKAQIAFWLLGATDGHAKNFSIFLYPGGGFELTPLYDVM
                     SAQDLHDKKQIQRKGMKLAMSVGDNRHYHIHEIHPRHYRQTADRAGLPDGMADMALEQ
                     VADALPDAIEAVCAALPVGFPAKLRDSVAKGALKRRDIIAAATTPN"
     gene            complement(12508..12813)
                     /locus_tag="VF226_18745"
     CDS             complement(12508..12813)
                     /locus_tag="VF226_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076082.1"
                     /translation="MPPKDPEYPYHDRTVRVIRCGRICFGRLKVNLSNVFAGQLMGLR
                     EVEDQFWLVSFLGYNLGFFDTQKGWMEPASNPFVPDRVLTMCPACWPVFSSFRTCFG"
     gene            12946..13362
                     /locus_tag="VF226_18750"
     CDS             12946..13362
                     /locus_tag="VF226_18750"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HEX7076083.1"
                     /translation="MTPSSHTSSVKAIRASPFCAAAAIAGFLALAACAATPQPPNRAL
                     QAAELAITDAERTRAAEYAPAELNQARDKFAAARTAVRNEQMVEAETLAEESRVHADL
                     ASAQAEMIKARAVNVEMQKSIDTLKQEMQRSSGERP"
     gene            13437..14243
                     /locus_tag="VF226_18755"
     CDS             13437..14243
                     /locus_tag="VF226_18755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7076084.1"
                     /translation="MTAPQGADTARSKLTELKADSELASRAQLEIKEAETAVKAAEVP
                     REDGAHARHLVLMADRKVDIARARAQSRLYEDQREGLSEQSERARLDSRTREADRAHS
                     NAESARADAATARTEADAARLKAEAMKRESDDLQRQIADLNARETDRGLIVTLGDLLF
                     ATGKSELKGSGAANLEKLAAFLHEYEDRSVMIEGHTDNVGSEESNLDLSQRRADSVRS
                     YLIRRGIGAARLSTSGLGEGSPIADNDSVTGRQQNRRVEVIISNAATASR"
     gene            complement(14401..15483)
                     /locus_tag="VF226_18760"
     CDS             complement(14401..15483)
                     /locus_tag="VF226_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7076085.1"
                     /translation="MGRSAAEIVLSESEEAELKLWRRRRKTSTGLHVRAGIVLDCARG
                     YSGEEIAERHATSQQTVTKWRRRFVTDRLAGLSDAPRSGQPRHYGDDRVQEVLEATLN
                     RRPKNATHWSIRSLGAELGVSSNFVHRVWRAFGLKPHLSKSFKLSNDPHFVEKVRDVV
                     GLYLDPPDKALVLCVDEKSQIQALDRTQPSLPMNYGMPETHTHDYRRHGTTTLFAALD
                     VATGEVIGKLKRRHRSTEYLSFLRHIDQTVPDDLDIHLIVDNYGTHKSPKVNQWLLRH
                     PRFHCHFTPTYSSWLNLVERFFATLTEQQLRRGTHRSVPALEKAIRDYLEIYNQDPKP
                     FRWSKSADEIIDSVNSVLKRINGSGH"
     gene            complement(15561..16175)
                     /locus_tag="VF226_18765"
     CDS             complement(15561..16175)
                     /locus_tag="VF226_18765"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7076086.1"
                     /translation="MPLGLVLYESGGALAYVYFPTDSIISLLYVMEDGASAEIAVTGC
                     EGLVGISLFMGGETTPSRAVVQSAGSGYRLKAEFLKKEFQNGGALQHLALRYTQALIT
                     QMAQTAVCNRHHAVEQQLCRWLLLSLDRLRSNELTMTQELIANMLGVRREGVTEAAGR
                     LQKAGLIQYSRGRITVLDRAKLERRVCECYAVVKKEYDRLLGSS"
     gene            complement(16598..17329)
                     /locus_tag="VF226_18770"
     CDS             complement(16598..17329)
                     /locus_tag="VF226_18770"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7076087.1"
                     /translation="MFSAAGSSMSPTTSLPATNRLIAGLPRKERDRLLRSSDTVALSF
                     GQILCEPRQVFRYAYFPLTSFISLVAIVGGRQPLEMGLIGSEGLLGASLGLGVGTAPL
                     RAVVQGEGTALRITAAELRRQLRLSPRLMKTLHRYQYVLMEQLAKSAACTHFHRVDTR
                     LARWLLMTHDRAHADHFHLTHEYLADMLGVRRSGVTVAAGDMQRRKLLRYARGEISIL
                     DRKGLEAASCDCYEAMQADYARLLP"
     gene            17709..18881
                     /locus_tag="VF226_18775"
     CDS             17709..18881
                     /locus_tag="VF226_18775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7076088.1"
                     /translation="MLHEFLTLHRDELIERCGSKAVLRLASASANEAVLPNGVPLILD
                     QLIETLRAEHVTAMRGHIRIWGQFNSSQARQPRIDEAASQHGLELLRNGFPVEEVVYG
                     YGDLCQAITDLAVERNATIQAHEFRALNYCLDKAIADALISYSARPYVSCAADKLVQE
                     LHERLGVLAHELRNHLQTATAALAAIRAGNVGVAGATAAVLDRSITGMRNLVHRSLTD
                     VQAIAELPVRRQMIALADFMAEIQLSASLEAKARECAFVVEHVDRTLGICADRDMLFS
                     ALDNLLQNAFKFTRCHTQVTLKAFAAGDRVLIEVEDNCGGLPAGDVEQMFWPFTQNGH
                     DRSGVGLGLSICRRSVDANGGALSVRNIPGTGCVFTIDLPRQAVAKSRVESSLLVG"
     gene            complement(19048..19191)
                     /locus_tag="VF226_18780"
     CDS             complement(19048..19191)
                     /locus_tag="VF226_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076089.1"
                     /translation="MMQAYGIKSARKDVYSYKEFKYERLDDAIRYAEIDRKRDQEKDG
                     SEV"
     gene            20570..20758
                     /locus_tag="VF226_18785"
                     /pseudo
     CDS             20570..20758
                     /locus_tag="VF226_18785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(21065..22414)
                     /gene="nhaA"
                     /locus_tag="VF226_18790"
     CDS             complement(21065..22414)
                     /gene="nhaA"
                     /locus_tag="VF226_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX7076090.1"
                     /translation="MNRPRKRGKTSRLTSAELGRLPSELADRWTKPFARFLRIETLGG
                     GVLLLSAAVALALANSPWADRYEGFWEIPFGFRLGSFELARSLRGWINDGGMTLFFFV
                     VALELKRELVLGALRQARSAALSIAAALGGMLVPAALYLALQWGQPGERGWGTVMATD
                     TAFVIGCLALLGSRIPLSLRIFMLSLAIVDDIGAILVVAIGYSSGIDWWLVAAAAAGV
                     AVIRAMAVVGLRGFPIYFLVGALVWVSVDASGIHATITGVVLGLLTPARRWVSDERLH
                     AILDRVVAHPTSAEGRGHTPDRHTLRLAERAARETLPPAERLEMALHPWVGFLIMPVF
                     ALANAGMPLQFGDLDNSVTVAVVVGFTLGKPIGILSFTWLSLRLRIAARPPDLDWPLI
                     AGGSLLGGIGFTMALFIANLAFEGSLLDSAKLGILLASTASALTGVALLGWSARVAR"
     gene            complement(22590..22868)
                     /locus_tag="VF226_18795"
     CDS             complement(22590..22868)
                     /locus_tag="VF226_18795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7076091.1"
                     /translation="MVAVHPGRILKRELAVRDLSANKLALALHVPSGRITSILNGKRA
                     ITPETALRLSRYFGNSAQFWMNLQTRYDLLEAERELGKRIDSEVGHVA"
     gene            complement(22876..23163)
                     /locus_tag="VF226_18800"
     CDS             complement(22876..23163)
                     /locus_tag="VF226_18800"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7076092.1"
                     /translation="MIRAWANRSSQQFYEQGKSSRFRGLDIDAANELLAVLDAATSLN
                     DLSPLKSVGLHKLTGNRKGQWAMTVNGPWRICFVFRKGDAYNVEIVDYHKG"
BASE COUNT         4728 a         7399 c         7130 g         4490 t
ORIGIN      
        1 gtccacgcaa cactcggttc cggctggtgg ccaacctttg ccggctcagg acttgcacct
       61 gttgggtcgc aaagaaagtt tccgtcatat gcatccgtct atatgacttc ctccttcacc
      121 aagctttgcc tggcgcaata aataattgat tcagctggca attaccgcag ggcgctcgtt
      181 acaattagag taccgtgcac tacggtgcat gactcaatcc cgcaaaaatc ccgcacggat
      241 ctcagaatcc aaatcgtcgc gggggcgctc gttgcgtgta tcgccgtact acgcacagag
      301 acgcgtcatt gagtcacgca gattcccgca gtccaccctg gctgctaaag gtccgacggg
      361 gtgaacctga gtcattcctc gccaccggac ggctctatct cctttatgac ttctataatc
      421 cctggattgt tctggtctgc gcagacgcga actcgatagc tgtgatgtgc acgaaggagg
      481 tactcggaaa agatcacgtc cacctttcct gagcaagggc tatcggacgg gagtacttca
      541 ttgagtaatt cccggatcag cgcgcaagcg caattgccca tcaagatcct ctcgtcacct
      601 cgtaacgacg ccttcgcgcc gttcgttcga cctgctacaa agaactaagg tcttcgccgc
      661 aaactcacgg aaggtcgaac gggccggcat tcggatagcg attcacgcta tgaatgataa
      721 tcatccagcc gtcaagatct tccgcgtttc gagcgtggcg cgtcgacccg ccgtgaagcg
      781 gtgagcccgt tccacggcgg gtcggctctg ttactccacc tcccgctccg attccgcctt
      841 cagctcgaaa tacgccagga aaagattccg gtattgggac tcctgccggt cgatgcgcgt
      901 gtgcgcctcc cgcagcttct ctttcacgac cttcaggacg gcttccgtct gcatcgcccg
      961 gctgtcaccg tcgtcctcga tggcggccat gagcaccgcc agcaggttct cggcgtcgtc
     1021 gagcagggcc atcgcctcac gctgctcttc ggtgtgcgcg tcggcggcat cgaaggcctc
     1081 cgacctgggc tcgtagtgct cgtcctctgt cgggtctgga atatcgtact cgcccccttc
     1141 ctcccggatc tgccggctgg ggaccagacc ttcgcacgaa gacctcgcgc ggcaggcggt
     1201 tagaaaggaa taggcgcgga ctcccggccg cgtcgtcgcg ccggcgacgg ttcgagcggc
     1261 cacgatggtt agacgggccg ggcggcgggg aatgtcacag actgcagggt tggcaatgct
     1321 catgggtctg gtcctccggt tgaggccagc tcagatgagt ggtgacgtca tcgacattgg
     1381 caaaaaccgc gagcggatcc gcatcggcgg tatcggcggc tgtcacggtc cgtccgacta
     1441 cgagcatcgg tctcgtgatc tacagggact ggcgcagcgt cacttcacgc aggacgagtg
     1501 tgagcgtctg agcagccaag gggggtgtct ggagacgcaa ggatggctcc gtccgccgtt
     1561 cggtaccgga gcgaagctga aggccttgcc caggcggttg cccgcacccg cccgcgggtc
     1621 tgcttttttg gccaccacca cacccggctc gacgcggagg tcgccggcgt tcgttgcatc
     1681 ggactcaaca aggtggcgat gccgggcaac ctcgtggcgc tcggcgcagg accgcggcta
     1741 cgagatcttg ggcgaatggt cgcgtcgccc ggacatcgct tctcgaacgc gttcgtacag
     1801 cgctcgcgag aaacccggtg accgtcctca cgggtcctcg ccaatgcggc aaaacgacgc
     1861 tcgcgcggga gctgctcagc gaggattcac cgaactattt cgacctcgag gatccggtca
     1921 gcctgactcg tctcgaggaa cctcgcactg ctctcgagct ccttggcggc ctgatcgtca
     1981 tcgacgaggt gcagcgacgt ccggatctgt tccctgtact ccgcgtgctg gccgaccggc
     2041 ggcaacgcaa gacgaaattc ctgatactgg gcagcgcatc gggcgaactt cttcggcaaa
     2101 gcgccgaatc gctcgctggc cgaatggaac gcatcgagat cagtggcttt acgctcgctg
     2161 agctcggcgc cgatgcggcc cgcaagctct ggcgccgcgg cggcttccct cgcgcgtacc
     2221 tcgcggccaa cgagcgcgac agcatgacct ggcgcaagca gttcatccag actttgcttg
     2281 aacgtgattt cccgcaatgg ggcgtgcgcg ttccagcagc agcgttgctt cctttctgga
     2341 ccatgcttgc ccattaccac gggcagatct ggaacgcttc cgagcctgcg cgtgcactgg
     2401 gcgtgaaccc gtcgacgaca cgccgctacc tcaacctcct gaccgatgcc atgatgctgc
     2461 gccagttgaa gccctggtat gccaatctcg gtaaacgcca ggtcaagtcg ccgaaagtct
     2521 atgtgcgcga cagcgggctt ttgcaccagc tcctggggat cgacagcgaa aaggcgttgt
     2581 tgtcgcatcc gaagatcggg gcttcgtggg agggattcgt catcgagcag gtgctggcga
     2641 gtgagcccca tgacgaggcc tggttctggg caacgcacca gggcgccgaa atcgatctcc
     2701 tgctcaggcg aggcgaaaag ctgctcggaa tcgagtgcaa gcgagccgat gcgccgaggc
     2761 taacgccgtc gatccggacc gccatcgagg acctcggcct cgagcgggtc gctatcgtct
     2821 atccaggcag caagcgcttt gcgctacacg atcgtgtaga agtggtgccc ttggcgtcgc
     2881 tggccgagcc gggccagcta ttcgtcccgg cggcagcgtg aattcgaacg ccacagtacg
     2941 ctccgggtat cgacgtatgt acgccagcga acagaagcgt cgtaagaatt gactgagaat
     3001 tttccttcgt ccatgcatct cggcaccgac tcctattcac cgatgcgcaa agaaccactg
     3061 caccagaacg aaggaaccag cgcctcgatc cggtcaggcc gcagcctcat cggggcaacc
     3121 atgccattta cggtcgaatc cgtaaccagg agctggtggc acgtcggttc cactttcgtc
     3181 atgctggtcg gttcgcttgc cggcgccgcg ttggcacccg cctggccggt gcggttgatt
     3241 ctgtccgttc tggctgcgct cttgatggtg cgtgccttca tcacttacca cgactacatg
     3301 cacgggtcca tcctggcgcg ctcccgcctg gcgtggatac tgttccgaat ctattcggta
     3361 ctgtttctca cgccaccccg ttcgtggaag acgagccaca actatcatca cggacacgtc
     3421 ggccagacct cggcggcaac cgacggcgcg ttcccgatca tgactaccac gacgtggcgc
     3481 agtgcttcgt gggcgacgcg cgcgagatac cgcgcggtac gccatccgct gaccgtgctg
     3541 accggctatc ttacgatctt cttctttagc ctgaccttga tgccactttt caggaatccg
     3601 gcccggcact ggggcgccct cccggtactg ctcgcccatg gcgccctgat cgcgactttg
     3661 tggatgctcg gcggcctctc catggcgttc tatgtcgtat tgttaccgat gactatggcc
     3721 tcggcgctcg gcagctatct cttcttcgcg cagcatagct tcaagcgcat gcatatcatg
     3781 ccttcggaga cctggactta ctacagggca gcgatggaat cttccagcta catgcggctc
     3841 aacaagctca tgcagtggtt cacgggcaat atcggctttc accacatcca tcacctgaac
     3901 gtgcgtattc cgttctatag actggccgag gcgatggatg cgatcccgga gctccaatcg
     3961 ccgatgacga cgtcgctcac accgcgtgac atcgccgggt gcttccggag ttgtctctgg
     4021 gacgaggacc ggcagcgaat ggtctcatat cgcgaagcgt ctccgagcgg gggtctctgc
     4081 gagagcgcca ggcgttcgtc aggatgcccg ggataggccg gaccggaagc gctcagatct
     4141 cgcttccacc cttcggccca tcacgcctgg tggggtagcg gatcgcattg tcgagccgtt
     4201 cgtaccgaga tccggcgagc aaggcgcact cggccttgag cacgtattga gcttcaggag
     4261 atcgtcaatc tggcgcagtg cggctgacag ttgacagtcg ttgctcgcgc gaccactgcg
     4321 ctgtattgac gaaccggctg ctcaccgcag cgcgccgcct cgagcgaact tgaagaaaat
     4381 cagcaggatg acggcgccga ggaaggatac gaacaatcct ggcacgctga agtcgccctg
     4441 gttgatcgat ccggcaccga ccagtccgcc cagaagccat ccgccgagcg cagcgccaac
     4501 gatgccgacc acgacgttga ggatgatgcc ctggagtccg tccgttcccg tgaacatgct
     4561 ggccagccaa ccgatcaggc cgcctgccac gagccaaagg atgatattca ttgattgttc
     4621 ctcgccgcgc tgtcgcgcgt catgagctgt ttcgccaatc gtccattcac cgaaaccgat
     4681 tatcggctgt ttccggcgat ggctctgtgc gacggcgtac acaagaggcg cagtcacaag
     4741 ggggatgctg cgcaggtcgt caggaaagca tggcaaggta gcgggcgggc cagcggcgcg
     4801 cgcatcggga atcacagaaa cttgcaggtc gtcgatactg cggcttcgcg aggtataacc
     4861 atttcctgcg cagactcgaa caacaactca ggcgatttga cgcagagcgt gacgcctcgt
     4921 cgcggggcgt gcgtttgaat tctgtgccaa aagctacttg cctccaaggc cagcttcccg
     4981 ttcagctcga cttccaaggc aaaacttggt cgcagcatgg cgtccgcccg aatgcgtcac
     5041 cgccgcattg aacagatctc cggcatttgc cgcggacatc tctccccggc gccgctcatc
     5101 acagccggcc ggtgagtaga agaatcaaca gaatcagcaa caggaggccg ccgatgccgc
     5161 cgatggcgat gctcttcacc aacgccgcaa cgatgagcac gacgatgatg aggccgatca
     5221 gtccgatgcc catgtcagcc tctccccgcg gtcgtccgcc gggacacgtc cacggcgtag
     5281 agcctgtgca cggtgcgagc gcccagccaa ccgccggcaa tggcgacgat caggctcagg
     5341 gcgccggcga tgaatgcggc ccacgtcacc gcttgcgcgt aggtcgacgc ggtctccacc
     5401 gcctggtcca tctgctgctt tgcctcttcg atctgctggc tcatctcctg cgacacgccg
     5461 tcgacaacgg catctatttc acgctcgctg aggctggtgt tcacggccag cacatccttt
     5521 gcgcgttccg gctccccggc aagaatcggc cgcgcggcag ccatcagcgt gtcggcgtcg
     5581 atctcgtcga gcgcgcgccg gatctcctgc tgcgaagccg cgggcggcgc ggccgtggat
     5641 ggggctgcgg ccgtcgccgg ggtgtctcgg gccggcgcag gctccgcggc atccggccgc
     5701 tgggcctcgg tcagggcagc tgcgatttcc cgcttgatgc gcgcctgcac cgtggtcatc
     5761 agcggtgaat cggcaagccg gccggcttcg ccgccgcctg cttccgcgcc cgcgcccccg
     5821 atgctcgcgc cggccgaggc cgccgtgctg accaccgacg tgccggtctg gatcagcccg
     5881 cggatgcccc aggcgccgag caccagcatc agtgccgtgc cgacactcca caaggtgact
     5941 ccgtggagca tgccgattgc ccgctccgac ttgccggcca ggcgcgccgt gagcaacgac
     6001 ccgaggaaat aggcgagaaa ggccgtaacg agcatccaca cggtcgcgcc gatgccgagg
     6061 cccttgccca tcgcctccgt gtccgttgcg tcggcgacgc tcaagccgat cgcggagccg
     6121 agcatgaaca ggagccaggt cgccgccacg acgaaggcca ggccggccag gacggcgccc
     6181 cagctgatca ggggccgacg ctccgtatct gctaccagtg tctcttcgtg gattgtggcc
     6241 atggcaattc tccggtgagg tcctgttgcc gtgcaagctg catgccatcc cgcatgggcc
     6301 gagcgccaat cctgccccgc gccggtcccg gacgggcgcc gcgcgtgaat ccttacaagt
     6361 acgcggccgc ttgcagtacg gcgttggccg ggtgttacgc ttcagccggc gatgccgggc
     6421 gcactaccat gaaactccgc atcctgagcg atctgcacac cgagctcgcg gacttcatgt
     6481 caccggaaac ggatgccgaa gcggtgacgc ggtcctgctt cacgaggcga gccgggcatg
     6541 gctcgtgcgc caactcgagc agactttcga tggcccgacc gtcgtcgacc gtgtgcatca
     6601 gatactcgga gtagtggata tagcgctgtg tgctccagcg caccgaccgc agaacgtgcg
     6661 gcgcgccatg ctccatgcgt ggccgtgctg tgaatgattc caggatccca tggaaatgaa
     6721 gaatcggtga cgcacacgga gagcgtagcg tcgtgcggcc gtacagcaca cgtcatgcga
     6781 caaccagtta cttgatacca aggtggagcc ttttatgaac attgcaaaac tgctgctgct
     6841 tctgacagtg gcttcgttca cggtcacagc ctgtgatatg gatgacagcg cggcggagaa
     6901 taccggcgag gctatagacg aggtgggaga aaagatcgac gatgcggcga cagatgccgg
     6961 aaacgcaatt gaagatgcct gtgaagaagt caaagagtct gctgacgcag aggataccga
     7021 ttgctaaaga gtaggccgct tccagagttt ccaaagcgtc tgattggcag tgtgaaaccg
     7081 agccgcgacc agctcgtctc tgcttccccc gggtcgccgg ccacgtgaga tcgatcttta
     7141 ggatcactgc agcggaacgc accggagaag gccacttccg aaaccgtccg acggaaccag
     7201 ctaagtgcgc cagcgtacag acttgtgccc cgcctggatg gcaggctgcc tttttagata
     7261 accagcaaaa taacatcagc atgttcagag taaagcaatt gatagtcggc gccgcgctgc
     7321 tggcgactgc cgctggctgc gccagcatcc ggggcacgga caccatcgcg gacaccacca
     7381 ggaccggcgc gattcacgac gtgagttttg aagagcgtat gacgccggtc aacctgagag
     7441 tgcagccggg agacgaagtg cgctgggtaa ataagcggag cacaccggtg acggtcgagt
     7501 tcttgggcga cgctctggcc gatgtgacct gtcagagtgg attttcgagt ctcctccgaa
     7561 gacagcagga aacggccacg atagaaccga acgagagtgc cagcctctgc ttcgggagag
     7621 caggtacggt gacgtacaat gcccgtatgg agtcccccgt agccggcggt cagatgatcg
     7681 agtcgggcac gattcgcgtc ggccagtaat tcccgtccat tgcagcgcgc gcagtgacgc
     7741 taatgggagg gttcccgacg gcgtagacgt ttccaggtcg tcacgcagcg cgctgttcat
     7801 ctgttcgtgc gttcgccgaa cagcatctca aacgcaatgc ccgcgctcca attgggctcg
     7861 cggcgctcca tcaaggtctc gcgcggccag gtgatgctcg atcgcagttc gagaaacagc
     7921 cactcgaggt aaatgcggcg ccggtagatg gcccgcagcc catagtcggc aatggtgact
     7981 tctcggtcgg tctaaccgga gatcccagcc tcataggcca aggcgtgtcc gccgccgagg
     8041 ttctgataga gcgtcaggct cgagaaccag cgtacgccct cgctttcccc cgtgaatgta
     8101 cccgagccgg tccagcgcac gagaaagtcc tcggcgagca agcgctccag gtcgaatcgc
     8161 gtagtcgcac caaatccctc gccccttcgc cagaagatcg tttcgtccag gcgaagaagg
     8221 ttctggtcgc cgaacggtac gcccaggcgg taagtacctt tgacgtaggg ttccaggggc
     8281 cagcggagct tggcgccgat gtcggcgtcg aagtgccccc ccatccgccc gggccgacga
     8341 taccccaggc cgaacagcac tgcgtcgtcg tcccttcgtg caaattgccg gggcagcgtg
     8401 tcgaagtcat cacgcagctc ggtcacgtat tcgtcggggt cgagccgccc gacgaatgca
     8461 ttgaaacgct cactcagttg cggcagtcgt acgtgtacac ggaaccgaac gctgggatcc
     8521 cagtgatcac gctcgtccca aagcgctccc actgcgagac tgccatgcgt ggcctggtat
     8581 tcgtcatgga aacgcgcgtt gccgaaaaaa ccgtcgaacc gggcggccga caggcaaatc
     8641 gtcctgtaaa gccccgcctg cactcgatca atccaaggca gcccaccagg cttgtcctcg
     8701 caaaggtccg gagcgtcttc aggcggaagg cccgtgggct ggatgggcat cggtatgcgc
     8761 tcaacccacg acggcgtgac gtttgcgggc tcgaatggcg gtgtttcctg ccgcggcgac
     8821 tgagcagcga caacgcccgc aatcgcattc attccgagtg cggccactgc gaggacccac
     8881 gggtgtccga tcaagcagct ctgatccgat ggttgcatgc cagcctaccg ggatgcaccc
     8941 gggattgtat gcgcaaatct caacagcacg caccagacac tttcaggtca tgcgctttgc
     9001 gtcggcgggc ttgtgcgtgc ggggacggtt catgccggcg gtcagcacca tacgcatgcc
     9061 ttcctccatc gacatctcca gtcgctggag gcagcgctcc ggaagataca acgcgtagcc
     9121 gccgatctgg tagctcatgg gcaggtacac cgccagcagc ggttcgtcct cgagggtcag
     9181 ttccggtacc gcctgcgctg gtgccgtgtc ggtgatgaag ccgatcagac ggatatctcc
     9241 cggaagcgtc acaagaacga catgccggcg atcgtcgtcc ggtgcgcgag aaacgaacag
     9301 catcaaatcg cggacaccgc tgtacacggt cttgacgagc ggtatccgct caagaagccg
     9361 ggttccgagc gtgagcatca cgcggccgaa gacccaggca ttgagaacag cgcccagggc
     9421 cagcactgcc agcacggcgc cgataaggcc gagtccaggg aagtaccagt ccggtggcag
     9481 cagtgcctgc ccgattccgc tgaacaggga atcggcagcg gtaaacagcc agtacaccac
     9541 ataggcggtg accccgagcg ggattatcgc gaccaggccg gtcaggaacg tgcgccacag
     9601 acgggcgagg ggacgacggg tgcccggcgt gtatcgccgc tggcgcgggt ttcggtaaga
     9661 caccatgggg acgcgctcct ccgtgcccat tagcctctac ggctgacagg gtacccggaa
     9721 tgcccgaatc cgctcagctt gctggcaggc cttgtcagcg gctgtgccgg gccagccgtc
     9781 gagcaaccct acattccgcc agtgtcacac ggcttccggg ccaagagctg ataccgtgca
     9841 acacgatgcc ttgtcgcggt acatcgtggc gtccgccgcc tgcagcaact catggtagtt
     9901 ctgaccgtct gccgggaaag tggcgacacc gaggctcgcc gtcatgctga ccggtatgcc
     9961 atcgatctgg tatggagcag caagctcgac gatgaccgtg ttgatcaccg ccatcgcctg
    10021 gttcctgcgc tcgatgccgg tcaggagcac cacgaactcg tcgccgccga agcggcaggc
    10081 tgtgtcagac ctgcgaatac acgacgtcag gcgtcctcct acctgctgga gcagccggtc
    10141 cgcttccacg tgccctaacg aatcattgat ggccttgaac ccgttcaagt cgaggaacaa
    10201 caacacgacc tgcctgcgct gccgtgttgc acgcgccacc gcggaagcgg tcttgcagca
    10261 ggcggcggtt cggcaagccg gtcagctcgt cgtaattggc aacgcgactc gccctgatca
    10321 gagcttctgc aagccctgcc atttcctgcc ggagcgcccg attctgtctc agcagtgcgt
    10381 cgaacaggcg gatgaacgct tcgaggcgct gcacgagtga cccctgatcg ggctggacgc
    10441 ggctctggca cgcgtcgctc tcgccactga ccccattcat ttcggctgcc ccgttttcta
    10501 tttgcacggg agccgacgat accggccctc cgctacaacg tcggttcgct gccgcacata
    10561 cataaaagcg cagtcgttcg catccgaaaa caccttgaga tgccggtaac tttcgggtaa
    10621 ccggtttacg ccgaactgga attcgactgc cgctcaatgg gttgcatgag tggtgcggtt
    10681 ccgcccttct tatacgctat agcgtatacg tgcatgttat tcgctgtaac gtatattgac
    10741 gtgctatacg gtgaagcgta taaccagtcg caagaagttt gcgatgaatc aaagcgcgcg
    10801 gacagccaaa cagatcgggg cgattgttcg ccgcgcgcgc cgcaatgtcg gtctgacgca
    10861 ggccgacctt ggcaagcggg tcggcttgcg ccaggccacg atatcccggc tcgagaaggg
    10921 cgaagaagag accaaactcg gcacgctgct ggatgtgctg agcgcgctcg gcctggagat
    10981 cacaatcgcc aggcgcggca agacctcggc ccggaacgtt gaggacatct tctgatggcc
    11041 aggaagcgca cgcgcatacc gctgaatgtc tatatcaacg gtcggctcgt ggggcggctc
    11101 cggcgcgaaa cttcgggcgc cattgacttc cactacgacg agagctggct gacttgggaa
    11161 cacacgtttc cggtctcgct gtccttgccg ctgcgcgagg atcgctacgt cggcgacccg
    11221 gtgatcgccg tcttcgacaa cctgctcccc gatagcgagg acattcggcg ccgggttgcc
    11281 gcacgcagcg atgctgacgg catcgaccct tacagcctgc tttcagccat cggccgggac
    11341 tgcgtcggcg cgctccggtt tctgccggac ggcaaagatc ccggcaaggc cgatgaaatc
    11401 gacgcccgga cgatcggtta tgaagaaatc gccgatcgcc ttgccaatct tgccaacgcg
    11461 ccgctcggca tgggtgacga cgccgaattt cgcatttcca tcgcgggcgc acaggacaag
    11521 accgcgttgc tctactggca ggatggctgg cacgtgccgc acggcggtac ggcgaccaca
    11581 catatactga agccgcagat cggccaacgc gggaactacg atctcagcca cagcgtcgag
    11641 aacgagcaca tgtgcctcga aatcatgcgc gcgctcggtt tgccggcggc caggacggaa
    11701 atcgccgact tcgccgggac acgtgccctc gttatcgaac gcttcgaccg tgaatggacg
    11761 aaagacggac ggttgttgcg cgtgccccag gaagattgct gtcaggcctt gtcagtgccg
    11821 cctacccgca agtaccaaag cgacggcggc ccgggcatgg tcgaagtctt cgcgcttctg
    11881 aaggcaagtg acgatcctgc ccacgaccag ctcgtcttca tgaaggccca aatcgctttc
    11941 tggctgcttg gcgccaccga cggtcacgcc aagaacttca gtatctttct ctatccgggc
    12001 ggcggcttcg agctcacgcc gctctacgat gtcatgtcgg cgcaggacct tcacgacaag
    12061 aagcaaatcc agcgcaaggg catgaagctt gccatgtcgg tcggggacaa tcgccactac
    12121 cacatccatg aaatccaccc gcgccattat cggcagacag cggatcgggc agggttgccc
    12181 gacggcatgg ccgacatggc gctcgagcag gtggccgacg cgctgcccga tgccatcgaa
    12241 gcggtttgcg ctgcgcttcc cgtcgggttt ccggccaagc tgcgcgattc cgtcgccaag
    12301 ggcgccttga agcggcgcga catcatcgcc gccgccacga ctccaaactg aacgcgcgcg
    12361 tgtcggttct ctgtgatcgt ccgcaggaag cggagccctt tttcgcagac gtgccactcc
    12421 ggcatcttca agcccgaatg ccggaccgtt agccaaggag ttgcggattg agtcgccaaa
    12481 cggagaagtt gagcgccaac gcgaagctca cccaaagcag gtacggaatg aggagaacac
    12541 cggccaacag gccggacaca tggttaacac tcggtccggc acgaacggat tagaggccgg
    12601 ttccatccag cccttttgcg tatcaaagaa gcctaaatta tagccgagga agctgaccag
    12661 ccaaaactga tcctcgacct cgcggaggcc catcagctgg ccggcgaaga cgttcgagag
    12721 gttgaccttc agacgtccga agcagatgcg accgcagcgg atcacgcgga cggtgcgatc
    12781 gtgatacggg tactcgggat ctttgggtgg cacctatcct ggcctgcacg ggttacggaa
    12841 tcggctggta agtacgccag cgcacggaac tatcggcgtc agagggcgat actctccgcc
    12901 aatcaggtgc aatccgcatc tgcgtttcca accaggacac ttaccatgac tccttcatct
    12961 cacacttcca gcgtcaaggc gatccgcgca tcgccttttt gcgctgctgc cgccatcgcg
    13021 ggctttcttg cgcttgccgc gtgcgcagcg acgccccagc ctcccaaccg cgccctgcag
    13081 gccgcagaac tggcaattac cgatgccgag cggacgcgag cggccgagta cgctccggcg
    13141 gagctgaacc aggcacgcga caaatttgcc gccgcccgga cggcggtccg caacgaacag
    13201 atggtcgagg ccgaaaccct cgccgaagag tcgcgcgttc acgccgatct tgcctccgcc
    13261 caggcggaaa tgatcaaggc aagggcagtc aacgtcgaaa tgcagaaaag catcgatacg
    13321 ttgaaacagg aaatgcaacg cagctcagga gagcgaccat gaacgcttgg gtacaccgat
    13381 ccggaatcac cgttactgcc gccgcattac tcgtaatcgc agcgtgcgcg acgccattga
    13441 ccgcaccgca aggtgccgac acggcccgca gcaagctgac ggagctcaag gccgattcgg
    13501 aactggccag ccgcgcgcag ctggagatca aggaagccga aaccgcggta aaggctgccg
    13561 aggtgccccg cgaggatgga gcgcacgcca ggcatctcgt gctgatggcc gaccgcaagg
    13621 tggacatcgc ccgggcccgc gcgcagagcc gcctctatga ggaccagcgt gaggggctga
    13681 gcgagcagag cgaaagggct cgcctcgact cgcgcacacg cgaagcagat cgggcccata
    13741 gcaatgccga gtccgcacgc gcggatgcgg ccaccgcccg caccgaagcc gacgccgcgc
    13801 gcctcaaagc ggaagcaatg aaacgcgagt ccgacgactt gcagcggcag attgccgacc
    13861 tgaacgcacg cgagaccgat cgtggtctga tagtgacgct cggcgacctg ctgtttgcaa
    13921 ccggcaagtc cgagctcaag ggcagtggcg ccgcgaacct cgaaaaactt gctgccttcc
    13981 tccacgagta tgaggatcgt tccgtcatga tcgagggcca caccgacaat gtcggcagcg
    14041 aggaatcgaa cttagacctg tcgcaacgcc gcgccgactc ggtacggtcg tatctgatca
    14101 gacgggggat cggggctgct cgtctctcca cctcgggtct gggcgaaggc tctccgatag
    14161 cggacaacga ctccgtcacc gggcgccagc agaaccggcg cgtggaggtc attatttcga
    14221 acgcggccac ggcctcgagg taaattgcaa gatcaagccc ctgccggccg acgaacctgc
    14281 aggaacgtga acccgcgcaa ctggctgcgc gggttttttg ttgtgcggga gcgcagcgag
    14341 ccagtcgcgc gcatgtcaga cgggcagcgt ggggaaggga ttggcgattg aaggctctgc
    14401 ctagtgtcct gatccgttaa ttcgtttaag aacgctattg acagagtcaa tgatttcgtc
    14461 ggccgatttt gaccatcgga atggctttgg atcctggtta tagatttcga ggtaatcgcg
    14521 gattgctttc tccagtgctg gcactgatcg atgagtgccg cgtctgagtt gctgttccgt
    14581 caaggtggcg aaaaagcgct cgaccaggtt taaccaggaa ctgtaggtgg gcgtgaagtg
    14641 gcagtgaaac cgcggatgtc ggagtagcca ttggttcact ttcggcgact tgtgggttcc
    14701 gtagttatca acgatcaagt gaatgtcgag atcgtccgga accgtctgat caatgtggcg
    14761 taagaaactg aggtattccg tactgcgatg acgacgcttg agcttgccga tgacttcacc
    14821 ggtagccaca tccaatgccg cgaacagcgt cgtcgtaccg tggcgccgat agtcatgtgt
    14881 gtgcgtttct ggcataccgt agttcatcgg caacgacggt tgggttcgat ccagcgcctg
    14941 aatctggctt ttctcatcca cacacaaaac cagcgcttta tcgggaggat caagatacag
    15001 gccgacaacg tcgcggactt tctctacaaa gtgcgggtcg ttcgacagct tgaagctttt
    15061 cgacaaatgc ggcttgagcc cgaaagcacg ccagactcga tgaacgaaat tgctgctaac
    15121 gcccagctcc gctcccaggc tgcgaatgct ccagtgggtc gcattcttcg gccgccgatt
    15181 gagagtggct tccagcacct cctgaacccg gtcgtctcca tagtgacgcg gctggccgct
    15241 gcgaggcgca tcagacagac cagcaagtcg gtcggtgacg aatcggcgcc gccacttcgt
    15301 caccgtctgt tggctggtcg cgtgtcgttc agcgatctct tcaccggagt aaccccgcgc
    15361 gcagtccaac acgataccgg ctctgacgtg caaaccggtc gaagtcttgc gacggcgacg
    15421 ccagagcttc aactccgctt cttccgactc gctcagtaca atttctgctg cactacggcc
    15481 catgctgtga ctcccattat ggaactcaca atacggcaaa ttctgcgtaa aatacttcaa
    15541 ttaattaacg gaacaggaca ttagctgctc ccgagcaggc gatcgtattc ctttttgacc
    15601 accgcgtagc attcacacac gcgccgctcc agtttcgccc ggtcaagcac agtgatgcgg
    15661 ccgcggctgt actggatcaa cccggctttc tgcaggcgtc cggcggcttc cgtcacgcct
    15721 tcgcggcgca cgcccagcat gttggcgatc aattcctgag tcatggtgag ctcgttcgac
    15781 ctcaggcgat cgaggctcag caacagccag cggcacagtt gctgctctac ggcatggtgg
    15841 cggttgcaca ctgccgtctg tgccatctgc gtgatgagcg cctgggtgta gcgcaaggcc
    15901 aaatgctgaa gtgctccgcc gttctggaat tccttcttga ggaactcggc cttgagccga
    15961 tagcccgaac ccgcgctttg cacgactgcc cggctgggcg tggtttcgcc gcccatgaaa
    16021 agcgaaatgc cgacgagccc ttcgcagcct gtgacggcta tttcggccga tgcaccgtcc
    16081 tccatcacgt acagcaggga aatgatgctg tcggtcggaa agtacacata ggccaacgcg
    16141 ccgccggact cgtacaggac caggccgagc ggcaaatcga cgggttcgag gttcggcaac
    16201 agccggtcgt aatccgtggc aggaaggcct gccagcaagc ggttctgccg ggggctcgga
    16261 tcggtaggca cttggacaac ctccaactgc cggaaggcca gaaggccaga aggccagaag
    16321 gccagaaggc atagatttta tgccgccaat tataccggcc atgtacgctg gcgtacattc
    16381 gtttccgcgg ctcttccagg gggcggcgcg cgcctctgtc gccgaagaaa agcccgatca
    16441 acgcatgttt gcgccgatcg ggcctgaagc gaactcacac cgggtttgtg cgtttgggga
    16501 catcacaaac gagtcttcgt gtgagcattc ttcgccagaa gtcagtcttt cacattaacg
    16561 gagttcgccg attggtccgt acgtcggcgc aagagactta gggaagcaag cgggcgtaat
    16621 cggcttgcat cgcttcgtaa cagtcgcagg acgctgcttc cagacccttg cggtccagga
    16681 tgctgatttc gccgcgcgca tatcgaagca gcttccttct ttgcatgtcg ccggcggcga
    16741 cggtcacccc gcttcggcgc actcccagca tgtctgcgag atactcgtgc gtcagatgga
    16801 aatgatcggc gtgcgcgcga tcgtgcgtca tgagcaacca gcgcgccagg cgcgtgtcga
    16861 ccctgtggaa atgggtgcag gcagccgatt tggccaattg ctccatcagc acgtactggt
    16921 accggtgcag tgtcttcatc aaacgggggc tcagtcgcag ctgacgcctc agttccgctg
    16981 cggtgattcg cagcgcggtg ccctcgccct gcacgacggc gcgcagtggg gcggtgccca
    17041 cccccagccc caggctagca ccgagcaagc cttcactgcc gatcaggccc atttccaaag
    17101 gttggcggcc gccgacgatt gccaccagcg atatgaagct cgtcagcgga aaatacgcgt
    17161 accggaagac ctgccgcggc tcgcaaagaa tctgcccgaa ggacagcgca accgtgtcac
    17221 tgctccgcag gagccggtcc cgttccttgc ggggcagacc tgcgatcaga cgattggtgg
    17281 ctggcaaaga cgttgtcggc gacattgatg atcccgcagc actgaacaac cagtattggt
    17341 gcgaggcagg tgcatggaca atctcattgc agtgttacct tcccctgtgc ggcggtctgt
    17401 gcgcccgggc acatagggag atcgccctat tccgcctcaa gatgtcgctc caaccacttg
    17461 gagaccttct caccccaatg gatggaatag ttcggggaca gcacccagcg actctcgtcc
    17521 ggaaagaggt cgaaggcctg cgcccagcgg ccgctcagcc tctatgttcg agaccgcacg
    17581 gaactgcccg agggaccgtg gcaacattcc ggaacgaatg agcgatccga tggcgaatgt
    17641 gacagacggg attctcgagc gagggcctac ggggtggaac ggctaagagc tgcctgtggg
    17701 gaggagcgat gctacatgaa tttcttacgc tgcaccggga cgaactgatc gagcgctgcg
    17761 ggtcgaaggc cgttcttaga ctcgcctccg cgagcgctaa cgaggcggtg ctgccgaacg
    17821 gcgtaccgct gatcctcgac cagctgatcg aaacacttcg cgcggagcat gtgacggcga
    17881 tgcgtggtca tatccggatc tggggtcaat tcaactcgag tcaggcgcga cagccacgga
    17941 tcgatgaagc ggcatcgcaa cacgggctcg agttattgcg gaacggcttc ccggtcgagg
    18001 aagtcgtcta cggctatggc gatctctgcc aagccatcac ggatctggcg gtggagcgta
    18061 acgcaacgat tcaggcgcac gagttccggg ccctcaatta ctgcctcgac aaggccatcg
    18121 ccgatgcgct gatcagctac tccgccaggc cgtacgtttc gtgtgctgcc gacaagttgg
    18181 ttcaagaact gcacgaacga ctgggggttc tcgctcacga gttgcgcaat cacctgcaga
    18241 cggccaccgc agccctcgcc gccatcagag ccggaaatgt gggtgtggcg ggagcgaccg
    18301 ccgctgtgct ggatcgcagc attaccggca tgcgcaatct cgtacaccgc tcgctgaccg
    18361 acgtgcaggc gattgcggaa ctgcctgtgc gccgacagat gattgctctc gcggatttca
    18421 tggcggagat ccagctctcc gcctcactcg aggcaaaagc gcgggagtgc gcgttcgttg
    18481 tcgagcacgt cgacaggacg cttggtatct gcgccgacag ggacatgctg ttctcggcgc
    18541 ttgacaactt gctgcaaaac gcgttcaagt tcacacgctg tcacacccaa gtgaccctca
    18601 aggcctttgc ggccggcgat cgggtgctta tcgaggtcga ggataactgc ggcggactgc
    18661 ctgccggcga cgtggaacag atgttctggc cgttcacgca aaacggccac gacagatccg
    18721 gtgttggact gggactgtcg atctgtcggc gcagcgtcga cgcaaacggc ggcgctctca
    18781 gcgtccgcaa catacctggc acaggctgcg tctttaccat cgatctgcct cggcaagccg
    18841 tggcgaaatc gcgggttgag agcagcttgc tggtcggcta gcgcgtcagc gctgcggatc
    18901 gtccgccggc tggtcgtgcg ggacagacct gcgatcagac gattggagac cggcagaggc
    18961 gatgccggcg acattaatga tcccgaagca ccgaaatagc ccggattggt gcgagactgg
    19021 gcgcaggaca accccgcgca gttgacttca aacctcgctt ccgtccttct cttgatcgcg
    19081 tttcctgtcg atctcggcgt agcggattgc atcgtcaagc ctttcgtact tgaactcctt
    19141 gtacgagtac acgtccttac gcgcggattt tattccatag gcttgcatca tttttttctc
    19201 gtcgtcattc atttcggctg tccattacac accctctctc ttgccttcgg tagggttcgt
    19261 tacagttcca gtcattgccg gaaaaattga gatgggcatt ctgcggcagc tcaacagcga
    19321 cgcacgatag cccgtccact cgataccctc gatcgcaagc ccagccaggg ccatacgtcg
    19381 agttagacag atagccatgt gctggaaccg caattgcgac gcaggcgcct gcgagtgccc
    19441 gatatcctcg atcgcatctc cagccggagt ctgcgtagga gtccgttata tatccgttgg
    19501 caggcactga aatggctgag caagcgccat cgaccgcccg gtatccccgc tcgcacgtcc
    19561 agccggatcc gtaagtggat tccgtcaggt atccgttggc aggtactgcg ataacctcgc
    19621 atccgtcatt gatctcgcgg tatccgcgtt cgcatttcca aatgtcgcct gttgaagcgc
    19681 tcaggtaggc atttgccggc agcacgactg cgacacaggt ttcgccgttc ttcttgtaac
    19741 cacggttgca ctgccaacca acgccgtagg acgaatttgt cgagtacgcg ttgtctggca
    19801 cgaacacggc agtacaagca cgatcaactt cacgaaaact gcgatcacat tcccacccgg
    19861 tcccatatct cttggcggtt gcgttcggtg gaatctcagg gctgacgccc tccgcgtgcg
    19921 aaatttccgg ggcgaccata gaaagcgcac attgaaacag gatgcaaagt gagatgttgg
    19981 ttcctgtaaa tatcttggcc atcagttctc gctccttcga ggttcagaac ctccgtactc
    20041 gatcattagg tatttcaact agcgctgctt acggcatgcc cttgcgctgt aaggcaaggc
    20101 aaatgttccc gcttcgctgg ttctgccagg ccttcgtttt cggcccgttc aagcgcttta
    20161 cgatctgtgt cctcactgtg ccgatcggcc gtcctgctcc gtttctgttc gtcggttccc
    20221 ggagtatcct gagtcgttga gggccgctga ggccccggag ttgccggcgg caacgcgttg
    20281 caacccgatc cctgttccat tgatggagtt gcagcaggcg gatatctggt attcatactg
    20341 tcactctgcg ccctgtcgcg aaattcctct gtgcggcagc atacacagcg tcagcgaatt
    20401 gttatcgact gcagggatgg ccggcaagaa ggcacgctga cacacgtcag ctacatcggg
    20461 agggggtcaa ttttatttcg acgatttcgg tcaaaaaggg ggcaattttc aaccgacgtt
    20521 gacatggaag aatcgatcgc cgtcgaagcg agcgcgggag gacatcgcct tgacggccaa
    20581 aaatccacgc gatccatcga cgttccgacg aagcctacgg tgcccccaac ttgctgacga
    20641 tcacggcatc cgggtcggtc gcaagcgggt cgcccggtta atgtgggccg cgggcctcag
    20701 gggcctgcag ctaccgaagt tcgtgaccac caccgtcgcg aatccggcgg ccgagcgcgc
    20761 actcgacaca gtcgacgggg agttcagcgc agtgggtcgc gcgtgtcaac ggtgatccaa
    20821 tattcccggt tgtggtcatt gaaaattccc cacccgggtt gaatcactga gttcgccggg
    20881 ctcctccgcc cgaaccaggc cagccttgag cttgtccttg aggcacggga tttcgattgt
    20941 gctcgtgcac tctgtgccag ccatggccgt gcggccgatc cgccgcttcc cctactcgga
    21001 tcaactgcgt tttggagccg tgcttcacgg ccgacgttcg gccatcgaca acggccatgc
    21061 atcatcagcg cgccacgcgt gcggaccatc cgagtagcgc gacgccggtg agcgccgaag
    21121 ccgtggaagc cagcagaatg ccgagcttgg cgctgtccag aagactcccc tcgaacgcca
    21181 agtttgcgat gaacaatgcc atcgtgaacc cgattccccc cagcaggctg ccgccggcga
    21241 tgagcggcca atcgagatcc ggcggacgcg ccgcgatgcg cagtcgaagc gagagccagg
    21301 tgaacgagag gattccgatc ggtttcccga gcgtgaatcc gacgaccacc gcaaccgtga
    21361 cagagttgtc gagatcgccg aattgcagcg gcattccggc gttggccagc gcaaataccg
    21421 gcatgatcag gaatccgacc cagggatgca gagccatctc caagcgctcg gccggcggca
    21481 gcgtctcgcg tgcggcccgc tccgccagcc gcagcgtatg ccggtccggc gtgtgaccgc
    21541 ggccctcggc gctggtcgga tgggcgacga cgcgatccag gatggcgtgc aggcgctcgt
    21601 cgctgaccca gcggcgcgcg ggcgtcaaca agccgagcac gacgccggta atcgtcgcat
    21661 gaatgcctga ggcgtccacg ctcacccaca cgagcgcacc gacgaggaaa tagatcggga
    21721 acccgcgcag gccaacgacg gccatagccc gaatgacggc gacgccggct gccgctgcgg
    21781 caacgagcca ccagtcgatg ccgctgctgt aaccgatcgc gacaacaagg atcgcgccga
    21841 tatcgtcgac gattgcgagc gacaacatga agatgcgcag gctcaacggg atgcgagatc
    21901 ccagcagcgc gaggcagccg atgacgaacg ccgtgtcggt ggccatcacg gtgccccagc
    21961 cgcgctcgcc cggctgcccc cactgcagcg caagatacaa cgccgcgggc acgagcatcc
    22021 cgccgagggc cgcggcgatc gagagcgcgg ccgagcgcgc ctgtcggagc gcgcctagca
    22081 ccagctcccg cttgagctcg agtgccacca caaagaagaa cagcgtcatg ccgccatcgt
    22141 tgatccagcc gcgcaacgag cgcgcgagct cgaacgagcc gagccggaac ccgaagggga
    22201 tctcccagaa gccctcgtag cggtcggccc agggcgagtt cgcgagcgca agcgcgacgg
    22261 cggcggacag caagagcacg ccgccgccga gcgtctcgat gcgcaggaag cgggcgaacg
    22321 gcttcgtcca gcggtcggcg agctcgctcg ggagccgccc gagctctgcg ctcgtaagtc
    22381 ggctggtctt accccgcttc cgtggacggt tcaggacttg ggacctaagc ggctaataag
    22441 gacgtttgaa ggcttacgtt gatagcagca ataatgaaaa gaagaatgca cttgctatga
    22501 cgaatgaaat tacgtgcggg ttaggtttgt atacagtggc acgcagtaac acatcgcttt
    22561 cgtcgcgccg ccagcccgtg aatgttcctt catgcaacgt gcccaacctc agagtcgatt
    22621 cgctttccta gctcacgttc agcctcgagc aagtcatacc gagtctgcag attcatccaa
    22681 aactgcgcgc tgttaccaaa gtaacgactc aggcggagcg cagtctcagg cgtgattgcc
    22741 cgcttgccat tgagtattga cgtaatccgg cctgagggaa cgtgcagtgc aagtgcaagc
    22801 ttattggccg acaggtcgcg tacggcaagt tcccgcttca aaatacgacc tggatgaaca
    22861 gctaccatct cttcatcacc ccttgtgata atcgacgatt tcgacgttgt acgcgtcgcc
    22921 ctttctgaat acaaagcaga tccgccatgg accgttaaca gtcatcgccc actgaccctt
    22981 acgattgccc gtaagcttgt gcagaccgac actcttcaaa ggactcaagt cgttgagtga
    23041 cgttgcggca tcaagcacag cgagcaattc gttcgctgca tcgatgtcca ggccacggaa
    23101 ccgcgatgat ttgccctgct cgtagaattg ttgactcgaa cgattggccc aagcccttat
    23161 catcccccga gagtactacg tcttgcatac tacgggaagc gtcgaagttg atctgctgcg
    23221 agccgcgcct gtcacaatgc gtgggtcgat ctgaacggtt aagccattga tgagtaagga
    23281 attgcgaaat tgcaactacc aggccgatgg ctgcttttcc agcagtggcg ttcggtttgc
    23341 aatggcaagt agttgattct cggaaactct gacatccgga gaacaccgat aaagaggcaa
    23401 gaactcccgc aaaaatcccg cagagatgct cagcaataac tcagaaagta atagtaatcc
    23461 gttgatatcg ttggtgccgg agagaggact cgaacctccg acccacgcat tacgaatgcc
    23521 ttttgagacc cttgccccgc ctcgtcttcc gcgtaacccc ttgttttatt gtgcgacctt
    23581 gtgcgacctt gtgcgggaat ctaacatcgc tcggcactta cagaggcact tacggcccga
    23641 acgggggagc tgcaaatggc aatgcgccgc accgactatc gccctcaggc gatcaccgtc
    23701 gcgctgatca ggcggctgga caaggcgcca ccgccgacga cgccacg
//
LOCUS       DASVDU010000281        11520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_742838, whole genome shotgun sequence.
ACCESSION   DASVDU010000281 DASVDU010000000
VERSION     DASVDU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11520
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742838"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF226_18805"
     CDS             <1..451
                     /locus_tag="VF226_18805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076093.1"
                     /translation="EGWTTAERMAKTTWEQRVEVLDRAHYVRYDYSTATYLGDTANLL
                     IERYDGDLRKLREAAERKPDQERRLLKEFKGIGDVGCDIFFREAQAVWSELFPFADKR
                     ALRAAEQLDLGKTPQELSKLVRRDQFVRLVGALARCDLERGYPEVML"
     gene            complement(487..1350)
                     /locus_tag="VF226_18810"
     CDS             complement(487..1350)
                     /locus_tag="VF226_18810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX7076094.1"
                     /translation="MASIAVAFAVMGLKYVAYWLTGSVALYSDALESIVNVVTAIAAL
                     IAVRVAERPADRSHQFGHHKAEYFSAGLEGALIVIAALLILIQAYDALMNPRAITAPA
                     EGLAVNALAGVLNAGWAWHLIRRGRALRSPAIAADGWHLLSDVMTSVGVIIGLLLALA
                     TGWQILDPLLAAAVAVHILWVGWRIVSQSMGGLMDAAVAGDMMDRIRQVIAANGDGAL
                     QIHDLRTRMAGRATFIEFHLVVPGTMPVTKAHEICDRLEESLTNEIEGAEVLIHVEPE
                     AEAKAKGALTF"
     gene            1601..2401
                     /locus_tag="VF226_18815"
     CDS             1601..2401
                     /locus_tag="VF226_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7076095.1"
                     /translation="MANFTTHIAVGTVVSGGLATLTLAADVIAPENLIAVTLAGVLGS
                     VLPDIDLKDSRPSRALFSGLAIFLSFCVLFAVAYRYSIAEMWLLWLGSFVLFRYGGEA
                     VFHRFSYHRGIWHSILAGLLFWFLTAIVFKNVLGRHEGVAWLAGGFLFVGYVTHLVLD
                     ELFSVDVLDTRFKSSFGSALKLYDGRKPGDTAVVAILAAILFTLTPSAKPFVDGISSR
                     ELWVGLQQRLLPQDKWFGLELLDKMADQPQPGSEMATGSIESKSEPGQ"
     gene            complement(2429..3052)
                     /locus_tag="VF226_18820"
     CDS             complement(2429..3052)
                     /locus_tag="VF226_18820"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7076096.1"
                     /translation="MGRRSTHTPEQLRELIIRSAHDIIAENGLGGMSAREIARRIGYS
                     PGTLYNLFDSLDDLVLQVEALLLDSLDQRLSELPNENSSDTQLNRIARTYLSFGRDHP
                     NLWNLISQHEIPPDMPIPSWYTERLERLVSRIEEALALQLPTGRDNPDSLKRSARVLW
                     AGLHGLTSLSTTKKLSSLAGNSADALVDELVNTYLAGLQKRGTGRQP"
     gene            complement(3289..6063)
                     /locus_tag="VF226_18825"
     CDS             complement(3289..6063)
                     /locus_tag="VF226_18825"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017273847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX7076097.1"
                     /translation="MSTEDVLSALGADPRQGLSTAEAQARLGLYGPNELAEKKPIPGW
                     LKFLAQFANLLIVLLLIAAVISTALWYYQDNAPLPYEALAIFAIVLLNAIMGYVQQAR
                     AEQALTALRRMSAAVATVLRDGEKQSIPASQIVPGDIILLEEGDRIPADARLIESISL
                     HTIEAALTGESQSVSKDTAPVREASSLGDQRDMVFGGTAVAFGRGRAVVTATGMRTEI
                     GQIAGMLGETPEEPTPLQRELDRVAKVLGVVVVAIALVIIATILLVGDVRGAAIFDVL
                     ILGVALAVAAVPEGLPAVVTAVLSIGVQRMARRRAIVRRLAAVETLGSADVIASDKTG
                     TLTRNEMSVRAVVTASGRITLFGTGYAPAGEICPENGASLDGSLKLELEKALTVAERA
                     NNATLRQVEGRWTIQGDPTEGALVVAARKAGFNSVELSRRYPRIAEIPFSSVRKLMST
                     IHDDAENPDHRILMVKGAPDVLLARCTHELVGEEVRPLTDERRAAIQEANEKLAGEAL
                     RTLAVAFRYLPKNAEEDFGENAERDLTFLALFGLIDPPRLEARDAVLRAKGAGIRPIM
                     ITGDHPKTAAIIASEVGIAPDARTVTGVEISQMSDDQLDRTLRDVSVYARVNPEHKLR
                     IVKALQRQGLTVAVTGDGVNDAPALKAADIGVAMGLSGTDVSREAADIVLADDNFATI
                     VAAIEEGRTIFANIRKFLRYLLSSNVGEVMTMFFGVLLADVLGLTAVGANGLVLPLVA
                     TQILWINLVTDGAPALALGLDPAAPDSMRHPPRPAGEGVLTLEMWLGIAFVGAVMAAG
                     TLLVLDWSLPHGLIEGSGTMAYGQTMAFTTLVLYQLFNVFNARSDSQSAFRELFANRW
                     LWAAVALSLGLQAVVIYVPPLQQAFSTTALSFGDWLICAAVASSVLWLRELSKSVKRA
                     WPRTTPKVTQ"
     gene            6678..6851
                     /locus_tag="VF226_18830"
     CDS             6678..6851
                     /locus_tag="VF226_18830"
                     /inference="COORDINATES: protein motif:HMM:NF025203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX7076098.1"
                     /translation="MRQHLMKVMFAVADQDGNGALSFNEVTEIHRRIFNAIDANKDGQ
                     VTMDELRNFMPGP"
     gene            7112..8119
                     /locus_tag="VF226_18835"
     CDS             7112..8119
                     /locus_tag="VF226_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX7076099.1"
                     /translation="MSLPETMAYIRLDGFGGPEVLKPDATTVPQPGEGEVLVKVAAAG
                     VNRPDVQQRLGGYNPPPGASPIPGLEISGEIVAVGPGCSRYRIGDKVCALVAGGGYAE
                     YCVAPEPQVLPVPRGLSMVEAAAVPETFFTVWTNVFDRGRLQPGETLLIHGGSSGIGT
                     TAIQLGREFGARVFCTVGNDEKARACEKLGAERAINYKTEDFVTVAKELTDGKGVDVI
                     LDMVGGAYVPRNIRALAVEGRLVQIAWLGGSKVEADFMMLMIKRLTWTGSTLRPRSVE
                     QKGEIARALEAKVWPLIEAGKVKPVMFKTFPLAEAAEAHRLMESSTHIGKIVLVTGEG
                     Q"
     gene            complement(8135..8593)
                     /locus_tag="VF226_18840"
     CDS             complement(8135..8593)
                     /locus_tag="VF226_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7076100.1"
                     /translation="MQLTKFSDFALRVLMYTHAAGDRIVTIEEIASSYCVSRAHLMKV
                     VNALTREGYLKAVRGRSGGLRLGKPAEEIRLGEVVKKTEPDFALVECFSTGNQCVISG
                     CCRLPRIMHEALDEFIATLNKYTLASIALRPQDFRPPVRPEGKVPSVVQT"
     gene            8703..9143
                     /locus_tag="VF226_18845"
     CDS             8703..9143
                     /locus_tag="VF226_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076101.1"
                     /translation="MQQKVELSKQFQVSSKDASGASGTSTELYDYDDGLLATGGPIMI
                     AAIAVALGIAATTFLASGEALFAVIISGFYTVMFFGIPAVMSRIRNGHDTRWQRAHRR
                     VDQVSILTGIMGRREAVLQMVMVPIGVAFAFAAFSTIWVLARPW"
     gene            complement(9156..10154)
                     /locus_tag="VF226_18850"
     CDS             complement(9156..10154)
                     /locus_tag="VF226_18850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7076102.1"
                     /translation="MAREIAEIPEAARRLIERADRIDALVDRILTAHPRTVIVCGRGS
                     SGHVGVYLRYLFEARVGQLVSSAAPSIVTAYQRPPDMRDALFILVSQSGRSPDLVTAT
                     EVARASGALTLAIVNDEHSPAASESDLVLPIAAGPEHAVAATKTVALSMLAGALIVAK
                     LTQDEDLLDALNRLPDRLQNALGCDWSQWGDSLVPAAAAFVAARGYGLGTAREIALKM
                     LESLRLPALGYSAAELRHGPRAAITPETPVLVLRQNDESALATDDLVHDLRQASETVF
                     VAGGPESTLPWIGDDHPVCDPIAMLIPAYNAIEAAARRRGLDPDRPEHLSKVTRTL"
     gene            complement(10184..11071)
                     /locus_tag="VF226_18855"
     CDS             complement(10184..11071)
                     /locus_tag="VF226_18855"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX7076103.1"
                     /translation="MHTEQFSPRYLDLDAWPTAEMIASMYEGQLVAAAAVQGALGAIA
                     AAVDDAVPALRRGGRIAYVGAGTSGRIGVQDGTELPPTYGWPADRLIFAMAGGLGALV
                     RSVEGAEDNEAAGAGAMADAGINGDDVVIGLAASGTTPYTIGALKAAGDVGAVTIAIA
                     NNPGAPLLEVARHPILVETGPEVIAGSTRMKAGTAQKIVLNLFSTAVMVRLGRVYRGL
                     MVHMRASNAKLKRRAERMVREIVGCSPDQASLLVEQADGDVKTAILLGFGLDPAQAAG
                     LLARHGGNLRSAIGEMMSS"
     gene            11292..>11520
                     /locus_tag="VF226_18860"
     CDS             11292..>11520
                     /locus_tag="VF226_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076104.1"
                     /translation="MSMQPGTTARHEAHAVLADRVFDGRHWHRNAAVLIEGARIRGVA
                     DWGEVPSDWPQHRLPVGANLAPGFIDLQVNGG"
BASE COUNT         2098 a         3575 c         3721 g         2126 t
ORIGIN      
        1 ggaggggtgg accacggccg agcgcatggc caagaccacc tgggagcagc gcgtcgaggt
       61 cctggaccgg gcacactacg ttcgctacga ctacagcacg gccacatacc tcggcgacac
      121 ggccaatctt ttgatcgagc gctacgacgg agatctgcgc aagctccgcg aggcagcgga
      181 gcggaaaccc gaccaggagc gccggctcct gaaagagttc aaaggcatcg gcgacgtcgg
      241 ctgcgacatc ttcttcaggg aagcccaggc cgtctggagc gagctcttcc ccttcgctga
      301 caaaagggca ttgcgggcag cggagcagct tgacctcggc aagacaccgc aggagctttc
      361 gaagctggtc cgccgagacc agttcgtgcg tctcgttggc gccctcgccc ggtgcgacct
      421 ggagcgcggg tatccggagg tgatgcttta ggtgcgagcc gcgaatcggg tcttcacgaa
      481 ttcgccctag aacgtcagcg cccctttcgc cttcgcctcg gcctccggct cgacatggat
      541 cagcacctcg gcgccctcga tttcattggt cagcgactcc tccagccggt cgcagatctc
      601 gtgcgctttc gtcacgggca tggtgccggg tacgaccagg tgaaactcga tgaacgtggc
      661 gcggccggcc atgcgggtcc gcaggtcatg gatctgcagg gcgccatcgc cgttggcagc
      721 gattacctgc ctgatccggt ccatcatgtc gccggcgacc gctgcatcca tgaggccgcc
      781 catggactgc gagacgatgc gccagccaac ccagaggatg tgcacggcga ccgctgccgc
      841 aagcaaggga tcgaggatct gccagccggt cgcgagcgca agcagcaggc ctatgatgac
      901 ccccaccgag gtcatgacgt cggaaagcaa gtgccagcca tcggcagcga ttgccggcga
      961 gcgcagcgcc cggccgcggc ggatgagatg ccaagcccat ccagcattga ggacaccggc
     1021 cagcgcgttg accgccagac cttccgccgg cgcggtgatg gcccgcgggt tcatgagcgc
     1081 atcataggcc tggatcagaa tgagtagcgc agcgatcacg atcagcgccc cttcgagccc
     1141 tgccgagaaa tactcggcct tgtggtgccc gaactggtga ctgcggtccg ccgggcgttc
     1201 ggcgacccgg acagcaatga gggcggcgat tgccgtgacc acgttgacga tgctttcgag
     1261 cgcatcggag tagagggcga cactgccggt gagccaatag gcgacgtact tcagccccat
     1321 cacggcaaac gcgaccgcga tgctggccat ggcgagccgg ccgagactgc ccacggctgg
     1381 tccttgcgat ggcagatgag ctgacgtcac aatcccgaaa ttcctgcaga aaggcccttt
     1441 atcgatcgct cggagcgccg atcaacgcgg cgaatccccg ctgctttctc cacagccgcc
     1501 gctccggagg tgttcacgga ctcaaccgaa tcagtgctta gctgccctgc cttggccgga
     1561 cgcgtattgc cgcaccaatc ggccgccctg ggggtgaggc atggcgaact tcacgacaca
     1621 catcgcggtc ggcaccgtgg tcagcggcgg gttggcgacg ctcaccctgg ccgcggacgt
     1681 gatcgccccg gaaaacctga ttgccgtgac gcttgcaggc gttttgggca gcgtgttgcc
     1741 ggacatcgac ctcaaggact cgcgtccgag ccgtgccctg ttttccgggc tggcgatttt
     1801 cctgtcgttc tgcgtgctgt tcgccgtcgc ctacaggtat tcaatcgctg aaatgtggct
     1861 gctgtggctc ggatcgttcg tgctgttccg gtacggcggg gaggccgttt tccacaggtt
     1921 ctcctatcac cgcggaatat ggcactcgat cctggccgga ctgctgttct ggtttctcac
     1981 cgcgatcgtc ttcaagaacg tgctcggccg gcacgagggg gtcgcgtggc ttgcgggagg
     2041 tttcctgttc gtcgggtatg tcacccatct cgttctggac gagctcttca gcgtcgacgt
     2101 tctggatacg cgattcaaat cctcgttcgg ctcggcgctg aagctttacg acggtcgaaa
     2161 gcccggtgat acggccgtcg tcgccattct tgcagccatc ctgttcaccc tgacgccctc
     2221 cgccaaacct tttgtcgacg gcatttcctc gcgcgagctg tgggtcggct tgcagcagag
     2281 attgctgcct caggacaaat ggttcggatt ggagctgctc gacaagatgg ctgaccagcc
     2341 tcaacccgga tcggagatgg ccaccggatc tatcgagtcg aaatccgagc cgggacaata
     2401 accttcaata gattaaagtt gtcggcaatt agggttggcg gccagttccc cttttctgta
     2461 acccggccaa atacgtgttc acgagctcgt caaccagggc gtctgcagaa ttgccggcga
     2521 ggctggaaag cttcttggtt gtcgataacg aggtcaatcc gtgcagtccc gcccatagga
     2581 cacgggccga tcgtttcagc gaatccgggt tgtctcgacc cgtcggcaat tgcaaagcca
     2641 gcgcttcctc gatccggctc acgagccgct cgagccgttc tgtataccag gacgggatgg
     2701 gcatgtctgg agggatctcg tgctgcgaga tcaggttcca aaggttcgga tggtcgcgac
     2761 cgaagctcag atatgttcga gcgatcctgt tcagctgagt atcggaggag ttttcatttg
     2821 gcagctcgga cagtctttgg tcgagcgaat cgagcagcag cgcttccacc tgaagcacca
     2881 gatcatcgag gctgtcgaaa agattataga gcgtgccggg agaatagccg atccgccgcg
     2941 cgatttctcg cgcggacatt ccgccaagtc cgttctccgc gatgatatcg tgcgcagacc
     3001 ggatgatgag ctcgcgaagc tgctcgggag tgtgcgtaga tcggcgcccc atgaatatgc
     3061 ctgaaccgat gtctttcagc tattcgctga tagataagtg cgaaatcgaa gtacgcaagg
     3121 aaatgatccg tataaagcag gattgggctc tgtggaagac gtaatccgct ggatgctttc
     3181 cagattcctg cgcattcatt tcggaaagcg cactttttcg ccccaatttt atcaaaatcc
     3241 ggcagttctg atggccagcg tttacgcaaa tgccacgctc gctttggatc attgggtgac
     3301 cttcggcgtg gtccggggcc atgcgcgttt gaccgatttg ctgagctcac gcagccagag
     3361 cacggagctc gccaccgccg cgcagatgag ccagtctccg aagctcaagg cagtcgtgga
     3421 aaaggcctgc tgcagtggcg gcacatagat cacgacggct tgcaagccta gcgacaaggc
     3481 cacggcggcc caaagccagc gattggcgaa cagctcacga aacgcgctct ggctgtcgga
     3541 gcgcgcgttg aaaacattga agagctgata gagaacgagg gtcgtgaaag ccatggtctg
     3601 gccgtaggcc atcgtcccgc tgccttcgat caaaccgtgc ggcaacgacc agtcgagcac
     3661 aagaagcgtt ccggctgcca tgacggctcc gacgaaggcg atgccgagcc acatttcgag
     3721 ggtcagaaca ccttcgcctg ccgggcgcgg cgggtggcgc atggagtcgg gagctgccgg
     3781 gtccagtccg agagcgagcg caggagcgcc gtccgtcacg agattgatcc agaggatctg
     3841 agtggcgacc aggggcagaa cgagcccgtt ggcgccgacg gcggtcagcc cgaggacgtc
     3901 ggcaaggagc acgccgaaga acatcgtcat gacctcaccg acgttcgaag agagcagata
     3961 ccgcagaaat ttgcgaatgt tcgcgaagat cgtgcggcct tcctcgatgg cggcgacgat
     4021 ggtcgcgaaa ttgtcgtcgg cgaggacgat gtcggccgcc tcccgggaga cgtcggtgcc
     4081 gctgagcccc attgccaccc cgatatcggc cgccttgagg gcaggtgcgt cgttcacgcc
     4141 gtcgcccgtc acggcgaccg tcaggccctg acgctgcaag gctttcacga tcctgagctt
     4201 atgctccgga ttgacgcggg cgtacacgga gacgtcccgc agcgtccggt cgagctggtc
     4261 gtccgacatt tgcgaaattt cgacgcccgt aaccgtgcgg gcgtccggtg cgatcccgac
     4321 ctctgacgca atgatggccg ctgttttcgg gtgatctccc gtgatcatta tcggccggat
     4381 gccggcgcct ttggcgcgca gcacggcatc cctggcctca aggcgcggcg gatcgattag
     4441 cccgaagaga gcaagaaatg taaggtcgcg ctcggcgttc tctccgaagt cttcctcggc
     4501 atttttggga agatatcgga atgctacggc tagggtgcgc agggcttcac ctgccaattt
     4561 ctcattcgcc tcctggatcg cggcgcggcg ctcgtccgtg agtggccgga cttcctctcc
     4621 gacaagctcg tgcgtgcacc gggccaggag cacatccggc gcgcctttga ccatcagaat
     4681 gcgatggtcg gggttctccg catcgtcatg gatcgtgctc atcagcttac gcacggacga
     4741 gaacgggatt tccgcaatgc gggggtagcg tcggctcaac tcgacggagt tgaaacccgc
     4801 tttccgggcc gcaaccacca gggcgccttc tgtcggatcg ccctgaatgg tccagcggcc
     4861 ttcgacctgc cgaagcgtgg cattgttggc ccgttcggcg accgtgaggg ccttctccaa
     4921 ctcgagcttc agcgagccgt ccagactggc gccattttcg ggacaaattt ctccagcggg
     4981 tgcatatcct gtgccgaata atgttatgcg gccgctggcc gttacgaccg cgcggaccga
     5041 catctcgtta cgggtcagcg tcccggtctt gtcagaggcg atcacgtcgg cagagcccag
     5101 cgtctcgacg gctgcgaggc gtcgcacgat ggcgcggcgc cgtgccatgc gctgcacgcc
     5161 gatggacagc accgccgtca cgaccgctgg caaaccctcc ggaacggccg cgacggccag
     5221 cgcgacgccg agaatgagca cgtcgaagat cgctgcccct cgcacgtccc ccaccagcaa
     5281 gatggtcgca atgatgacca gggcgatggc gacgaccacc accccgagca ccttggcgac
     5341 gcggtcgagc tcccgctgca gcggcgtcgg ttcctcgggc gtttcgccga gcatgccggc
     5401 aatctggccg atctccgtgc gcatgcctgt cgccgtcacc actgctcgtc cgcgtccgaa
     5461 cgcgacggct gttccgccga agaccatgtc ccgctggtct cccaggctgc tcgcctccct
     5521 gaccggggcg gtgtccttgg agacggactg actttcgccg gtgagcgctg cttcgatcgt
     5581 gtgcagcgag atggactcga tcaggcgggc atccgcaggg atgcgatcgc cttcctcgag
     5641 caggatgatg tcgccaggga cgatctggct agcgggaatg ctctgcttct cgccatcgcg
     5701 gaggacggtc gcgactgcag ccgacatgcg gcgcagcgcg gtcagcgcct gctctgcgcg
     5761 ggcctgctgg acgtagccca tgatcgcgtt gaggagcacg atggcgaaga tcgccagcgc
     5821 ctcgtatggc agcggtgcat tatcctggta ataccagagc gccgtcgaga tgaccgctgc
     5881 tatgaggagc aaaacaataa gaagattggc gaactgcgcg agaaacttca gccaaccggg
     5941 aatgggcttt ttttctgcaa gctcgttggg tccatagagg cccagtcgtg cttgcgcctc
     6001 cgccgtgctc aatccctggc gtggatcggc gccgagcgca gacaggacat cctcagtgga
     6061 catccgatga ggctcgaatc cttgattttt gcgcttttct aaaaccatcc cttgatcgcc
     6121 ttatcgctcg ctgacccttc gggccttcgg tcaaatgagc ggctcagatg aggcaatcgc
     6181 tccccaggtg cattgatcta gatcctcggt gccatccggt tcgatgtttc aaggcggtcc
     6241 agttgtccgg aactcgcgag agtcctgcag agttcgtgcc aaggcaggat ctacggttcg
     6301 acactgagga gagtcacatg aacatgcgat caggggcgtc agctgccgca atcatcctcg
     6361 ctgcgagcct tacatgggct ggcgcagcgc aggctcaaga gaactcgaat tcagcttcta
     6421 cgaccttggc tcaaacctcc caaatgccag ggatgaccgg gcagcagcag ggaacgcaac
     6481 ccggccagcc aggaatgatg ggaccgggta tgggtcaagg ccagggaatg gggccgggtc
     6541 aaggccaggg acagggaatg atgggccaag ggatgggccc ggggatgggt caaggaatga
     6601 tgggtcgggg tactggccag agcatggccg gcagggacgg gagagggcat ggcagggggt
     6661 ggaggaaacg gcgcggcatg cgtcagcatc tgatgaaggt catgttcgcc gttgccgacc
     6721 aggacggcaa tggcgctctg tccttcaacg aggtgaccga aatccaccgt cgcatcttca
     6781 acgcgatcga tgccaacaag gacggccagg tcacgatgga cgagctacgc aacttcatgc
     6841 caggcccgta gcccgccgcc ctctcggtcg gagagaaatt ccgaggattg ctggaactgc
     6901 caagtcgctt gtgcgttatg gaaacaagtc cgaaattcga gagatcgaag agtaggaccc
     6961 gggagccttc ggcgtaaaaa ccgagggctc ttgctttttg tgccccaaga tccgcctccc
     7021 cccaacagtc ctttccggca ctccttcgtg acgcgcgtgc ctcgttgcgc taagcaacgc
     7081 tgatggagag atcaagccgg aggatctacc gatgagcttg cccgagacaa tggcctacat
     7141 ccgtctcgac gggttcggcg ggccggaggt gttgaagccg gatgccacga ccgtaccgca
     7201 gcccggagaa ggcgaggtgc tggtgaaggt cgccgcggct ggcgtcaacc gtcccgacgt
     7261 gcagcagcgt ctgggaggat acaacccgcc gccgggcgca agcccgatcc ccgggctcga
     7321 aatctccggc gagatcgtag cagtgggccc agggtgcagc cgctacagga tcggcgacaa
     7381 ggtctgcgcg ctcgtggcgg gcgggggtta tgccgagtac tgtgtcgcgc cggaaccgca
     7441 ggtgctgccc gtccctaggg gtctttccat ggtcgaggcg gcggctgtgc cggaaacgtt
     7501 cttcaccgtc tggaccaacg tgttcgatcg tggccgcctg cagcccggag agacgcttct
     7561 gattcacggt ggctcgagcg gcatcggcac gaccgcaatt cagctcggcc gtgagttcgg
     7621 cgcgcgcgtg ttctgcacgg tcggcaatga tgaaaaggcc agggcctgcg agaaactcgg
     7681 cgccgagcgg gcgatcaatt acaagaccga ggacttcgtc actgtcgcca aggagttgac
     7741 ggacggcaag ggcgtcgacg tcatcctcga catggtcggc ggcgcttatg ttccgcgcaa
     7801 catccgcgcc ctggccgtcg agggccggct cgttcagatc gcctggctcg gcggctcgaa
     7861 agtcgaggcc gacttcatga tgctgatgat caagcggctg acctggacgg gctcgacgct
     7921 gcgcccgcgc tcggtcgagc agaagggcga gatcgcccgc gcgctcgagg cgaaggtttg
     7981 gcccttgatc gaggcaggca aggtgaagcc ggtcatgttc aagacctttc cgctggcgga
     8041 agccgccgag gcgcacaggc tcatggaaag cagcacgcac atcggcaaga tcgtcctggt
     8101 gaccggcgag ggccagtaag tcctcgatcc agcttcaggt ctgcacgacc gaggggactt
     8161 tcccctcggg acgaacgggc ggccggaaat cctgcggccg tagggctatg ctggccaacg
     8221 tgtacttgtt gagcgtggcg atgaactcgt ccaaggcctc gtgcatgatg cgaggcagcc
     8281 ggcagcaacc gcttatcacg cactgattgc ccgtactgaa gcattcgacc agcgcgaagt
     8341 cgggctccgt cttcttcacc acctctccga ggcgaatctc ctctgcgggc ttgccgagcc
     8401 gcaggccgcc cgaccgtccg cgcaccgcct tgagatagcc ctcgcgggtc agcgcattga
     8461 cgaccttcat caggtgcgcg cgcgacacgc aatacgagct ggcgatctcc tcgatggtaa
     8521 cgatccgatc gcctgccgcg tgggtgtaca tcagcacgcg gagggcgaag tcggagaatt
     8581 tggtgagctg catgtgacgc ttttatccgg ctgccctgaa tagattcagt cttgacacac
     8641 cttatccaga ggcacaacct ccaaatcgat gcatcctgca tgtatcatat aggagggtca
     8701 ctatgcagca gaaggtcgaa ctttcaaaac aatttcaggt ctcttcgaag gatgcatcag
     8761 gtgcttccgg cacctccacg gagctttatg actacgacga cggcctcctt gcgacgggcg
     8821 ggccgatcat gatcgcggcc atcgcggttg cactcggcat cgctgcgacg acgtttctgg
     8881 caagcggcga ggccctgttc gccgtcatca tctcgggctt ctacacggtc atgttctttg
     8941 ggatcccagc ggtgatgtcg cggatcagga atggccatga cacgcgctgg cagcgggcgc
     9001 accggagagt cgatcaggtc tcgattctca cgggaattat gggccgccgc gaggccgtac
     9061 tgcagatggt gatggtcccc atcggagttg ctttcgcgtt tgcagccttc tccacgatct
     9121 gggtgctggc ccggccgtgg tgacggcata cggccttaca gcgtgcgggt caccttgctg
     9181 aggtgctccg gccggtcggg atccaggccg cggcggcgcg cagcggcctc gatcgcattg
     9241 taggccggga tcagcatcgc aatcgggtcg cagaccgggt ggtcatcccc gatccagggc
     9301 agagtgctct cgggcccgcc ggccacgaaa accgtctcgc tggcttggcg caggtcgtgg
     9361 accaagtcgt cggtggccag tgccgactcg tcattctgcc ggagcaccag gacaggtgtt
     9421 tccggcgtaa tcgccgcccg cgggccgtgt cgcaactcgg cggcgctgta gccgagggcc
     9481 ggcaggcgaa ggctctcgag catcttcaag gcgatttcgc gggccgttcc gagaccatag
     9541 cctcgcgcgg caacaaaagc ggcggcggcc gggacgaggc tgtcgcccca ttgcgaccag
     9601 tcacagccaa gcgcgttctg cagccggtcg ggaagccggt tcagcgcgtc aagcaaatct
     9661 tcatcctgcg tgagctttgc cacgatcagc gcgccggcga gcatcgagag tgcgacggtc
     9721 ttggtggcgg cgacggcgtg ctcggggccg gccgcgatcg gcagcaccag gtccgattcc
     9781 gaagccgcag gtgaatgctc gtcattgacg atcgcgagcg tcagcgctcc gctcgctcga
     9841 gccacttctg tcgcggtcac cagatcaggg ctgcggccgg attgtgaaac cagaatgaac
     9901 aatgcatccc gcatgtccgg cggccgctgg taggcggtga cgatcgacgg agctgcggac
     9961 gagacgagct gccctacccg cgcctcgaac aggtatcgca gatagacgcc aacatgtccg
    10021 gagctgccgc gcccgcacac gatcacggtc ctcggatgcg cggtgaggat gcggtcgaca
    10081 agcgcgtcga tccgatcggc gcgttcgatg agccgccgtg ccgcttcggg gatctcggca
    10141 atctcccgcg ccattgccgt gggggctggg ttgtcagcaa tcgtcacgac gacatcattt
    10201 ctccaatggc ggaccgcagg ttgcctccgt gccgggccag gaggcctgcc gcctgggccg
    10261 ggtcgagccc gaagcccagc aggatcgcgg tcttgacgtc tccatctgcc tgctcgacca
    10321 acagggaggc ttggtccggc gagcagccga cgatctcgcg caccatccgc tcggcccggc
    10381 gcttcagctt ggcattgctg gcgcgcatat ggaccatgag gccgcgatag acgcgcccga
    10441 gcctgaccat cacagccgtc gagaagaggt tcagcacgat tttttgcgcc gttcccgcct
    10501 tcatgcgggt ggagccggcg atcacttccg gtcccgtctc gaccaggatc gggtggcggg
    10561 cgacctccag cagcggcgcg ccggggttgt tggcgatggc gatggtgacg gcgccgacat
    10621 cgcctgcggc cttgagcgcg ccgatggtgt agggcgtcgt tccgctcgcg gcgaggccga
    10681 tgaccacgtc gtccccgttg atgccggcat cggccattgc tcctgcgccc gccgcttcgt
    10741 tgtcctccgc gccttcgacg ctccgcacga gcgcccccaa gccgccggcc atcgcaaata
    10801 tcagccggtc ggctggccac ccgtaggtcg ggggcagctc ggttccgtcc tggacaccga
    10861 tgcggcccga ggtccctgcg ccaacgtacg cgatgcgtcc accccggcgg agggcaggca
    10921 cggcgtcgtc gacagccgct gcaatcgcgc cgagcgcacc ctgaaccgct gccgctgcca
    10981 caagctggcc ctcgtacatg gacgcgatca tctcggcggt cggccacgca tcgagatcga
    11041 gatagcgggg actgaactgc tcggtgtgca tctgctcctc tgcagggaat gaagcggcgg
    11101 ccacccgcac acggactccg tgcgccgtta acgcgtcatg cgtcgcgagg atgcgtcgcg
    11161 gcgccctcgc ttgacgtggg ttgtgcgtcc attgcacatg caattacagc gagcaagcgg
    11221 ggcggctgca gacgtatgcg ttgtggcgcc gcaccggagc acgcatcgct caaaccggct
    11281 aagagaatcc catgtcgatg cagccaggca caaccgcgcg acacgaagcg catgccgtcc
    11341 ttgcggatcg cgtgttcgat ggccgccatt ggcatcggaa cgccgcggtt ctgatcgagg
    11401 gggcgcgcat tcgcggggtt gcggattggg gcgaggttcc gtccgactgg ccgcagcatc
    11461 ggctgccggt cggagccaat cttgcgcccg gctttatcga tttgcaggtg aatggcggcg
//
LOCUS       DASVDU010000282        15151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1429665, whole genome shotgun sequence.
ACCESSION   DASVDU010000282 DASVDU010000000
VERSION     DASVDU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429665"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VF226_18865"
     CDS             complement(<1..317)
                     /locus_tag="VF226_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase PUA domain-containing
                     protein"
                     /protein_id="HEX7076105.1"
                     /translation="MAVHDFTAQRLFVEAGLRAGVEVPCTAEQANYLRNVLRLGAGAE
                     ILIFNGRDGEWRAQLAVEGKRSCTLRPLEQVREQEAGPDIHYLFAPLKRARLDYMVQK
                     ATEL"
     gene            464..1420
                     /gene="ubiA"
                     /locus_tag="VF226_18870"
     CDS             464..1420
                     /gene="ubiA"
                     /locus_tag="VF226_18870"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEX7076106.1"
                     /translation="MNSTTDTPNVIADAPPDNWVDRYAPVALQPYLKLGRFDRPIGSW
                     LLLFPCWWSLALAELSRGRPYPNLWYVLLFLIGAFVMRGAGCTWNDIVDRDFDGRVAR
                     TRGRPIPSGQVSVTQALLFGVALSLVGLAVLVQFNAFTIWLGIASLGLVAIYPFAKRF
                     TFWPQVVLGLTFKWGALVGWAAVFGQLSAAPIVLYAGCVLWTIGYDTIYAHQDKEDDS
                     ILGLKSTALRFGDASQNWIAAFYAGALVLWAAAAFLAGAHLIFYTALALVGVQLAWQV
                     ASLDIDDPANCLRRFRSNRDVGWALFLGLIADMAISALAGLA"
     gene            complement(1585..3450)
                     /locus_tag="VF226_18875"
     CDS             complement(1585..3450)
                     /locus_tag="VF226_18875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7076107.1"
                     /translation="MTFVHSISNSWPKQAAQAGASSASAAVDLGAMPEWKLSDLYAAP
                     DAPEVERDFDRAAAEAKRIKETYQGRLVAMGRDGAALARAVKDYEQLGDLMGRLGSYA
                     GLLYAADQSDPVRAKFFGDTSERLTNISNDLIFFELELNKIEDADLAEALKHPDLARY
                     KPWFDDLRKEKPHQLDEKLEQLFHEKSQTSRGAWNRLFNETMAALRFPVEGEPEPLAL
                     EPTLNLLTSADEGRRRAGAEALAKVFKENLRLFTLITNTLAKDKEISDRWRNFKDVAD
                     SRHLANRVEGPVVDALVSAVRASYPKLSHRYYKMKARWLGKDRLAHWDRNAPLPEKPE
                     RIVPWSEAQEIVLTAYNDFAPEMASIARKFFENGWIDAPVRPGKAPGAFAHPTVPSAH
                     PYVLLNYQGKSRDVMTLAHELGHGVHQVMAAPLGPLLSQTPLTLAETASVFGEMLTFR
                     ALIAEAKDARERKALMASKVEDMLNTVVRQIAFYTFERKVHEERRKGELTPDRLNEFW
                     LEVQGESLGPAIELKPGYEVFWTYIPHFIHSPFYVYAYAFGDCLVNSLYALYEEAHPD
                     FVTKYFDMLKAGGSKHHSELLAPFGLDARQPEFWQKGLNVIERMIDELEAMDRAA"
     gene            3699..5201
                     /locus_tag="VF226_18880"
     CDS             3699..5201
                     /locus_tag="VF226_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7076108.1"
                     /translation="MAKRVLIVDDDPAQRRILEECIKRFGFECKTAASGDQALHILAG
                     PEKDGIAIVLLDLVMPGTNGMTVLEQLRGKPGIPPIIVQTSHGSIDTAISAMRAGAVD
                     FVVKPASPERLEVSINSALKLQALSGEIARLKKKSEGTLTFADLIAGQAMQRVVSLGK
                     RAAGSQIPVLIEGESGVGKELIARAIQGESDRAGKPFVTVNCGAIPENLVESILFGHE
                     KGAFTGAVDKRIGKFQEADGGTLFLDEVGELPLEAQVKLLRALQDGEIDPVGAKRPVK
                     VDIRLISATNRDMIELVKSGRFREDLFYRLNVFPIWVPPLRQRLDDVPELAHHFLARF
                     AAEEGKHVTSISEQAMQLLTRYSWPGNVRQLENAVFRAVVLADGPELTVAEFPQIAMH
                     VEGFSAEIPAAPPPQKPAVYTGPAMIGADDKVPRTIELKGTDGGKAIGIPAVTESGDV
                     RTLEAIEADMIRLALGRYRGHMSEVARRLGIGRSTLYRKMQEYGLESRPN"
     gene            5372..7618
                     /locus_tag="VF226_18885"
     CDS             5372..7618
                     /locus_tag="VF226_18885"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7076109.1"
                     /translation="MMRPARTLLTGLALSTLLASAALASGEPDPGEASPAPAAVAEPV
                     SGNSEAVSATDAAAAPVAETSNSAEQAEDTADPAGPSAASGQQTGSDVTARHDADEAG
                     SSQTADVQPAASGAASEAEPDASIEPATAAASEPEKPVVEQDGSTVSTASTTTADPVA
                     GEAAEEQPEQATAGQEPAAQASDSLAITDPGVDDQAEPEPEQASTPDAPTEPQSPASA
                     EADPTNEAPETAPAAAAAQSGQPEQSSDTASAEQTIEVSQFEPELLRLISEAQDLSDA
                     EREALTEFYKGRGDDFLWVADGTFTPRAEAAMAEIRRADDWGLEASAFELPDAVESSE
                     PADVAAAELKLSLAVLKYAKHARGGRMDPRQLSNYIDREPPLLPPAEVLAGIAAADAA
                     DGYLRGLHPQHPQFEKLRQAWLDLRNGKLPAEDAEEDVTSKSRAKRKSRETNSAESKM
                     RKLLYNMEQWRWMPEDLGQFYVWANIPEFTLRVVKDGKIVFTERLVVGKADTQTPIFS
                     DEMETIVFHPFWGVPNSIKVKEILPGLIRGTNVLARNDLRIQYRGRDIDPWSVDWTRT
                     DIRNFHVYQPPGGKNVLGVVKFLFPNKHAVYMHDTPTKNLFSASQRTFSHGCMRVRNP
                     LKFAEVLLAYDKGWSEGRVLSLVRNGPRNNHVNVESKIPVHITYFTAWVDEDGKVRTW
                     PDVYGHEKRIQMGLEGKAHLIAKTKEDLGAVQAEVVGRLAETRPTWSSPSYSSSRQPD
                     WARRAFGH"
     gene            7911..9434
                     /locus_tag="VF226_18890"
     CDS             7911..9434
                     /locus_tag="VF226_18890"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX7076110.1"
                     /translation="MYKRGLSLLAGVAALVFVAVYGAPRLTAGLPQTRTISLYNIHTK
                     ETITVEYKKDGKYVPAAMEKINWILRDWRKNEATQMDPALIDLLWEIHTELGSEEPIH
                     IISGYRSRATNDMLRKTRGGQAKQSRHILGKAADVHFPDVPLRRLRYSALVRERGGVG
                     YYPTSAIPFVHVDTDRVRAWPRLPRYELALLFPDGRTKHIPADGRPLTPEDVHEARKH
                     HSELAAQVAAYQELRKAALSPTRIAKAEPVARDIPRPGSRIAAAWQQTEPQQPSHEPA
                     NAQEKPLPKLVAGPRPAIRPPRVANQPSDEDRMKLAQLAALASLSSVPSGARLMQASL
                     KADEPLPEERLTGSVPGTDQSSNTRLAALDFEAGGQPAPGRFHWGVGWTTERKEEIEP
                     TTPTGWAVAPEYDEEHPEELSYRPFPIIPYLTETPSPDDPALARMEHPDVAKTLELLD
                     NAGDLPVMQLRAEPQTAQLLWAQQFQGEAVPLASVFGARPPAAPSGLNERHVRLSAR"
     gene            9580..11709
                     /gene="ligA"
                     /locus_tag="VF226_18895"
     CDS             9580..11709
                     /gene="ligA"
                     /locus_tag="VF226_18895"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7076111.1"
                     /translation="MTTSAVSHKPVSALTPKEAEEELARLAAEIAHHDRLYYQLAQPE
                     ISDAEYDALVRRNAEIETRFPELIRPDSPSRRVGAPPVEAFGKVRHRVPMLSLSNAFS
                     EEEIIEFVDRVRRFLGMKPSETIEFTAEPKIDGLSISIRYEHGRLVEAATRGDGTEGE
                     NVTANVRTVRDIPHRITGPGCPDIIDVRGEIYLAHADFQKLNEAQAASGQKIFANPRN
                     AAAGSLRQLDPKVTASRPLKFFAYAWGEVSHLPARTQWEMYEVYRRWGFPTNPLARLC
                     RGPEEMLAFYREMSTRRATLGYDIDGIVYKVNRLDLQDRLGFVSRSPRWAVAHKFPAE
                     QAMTILRDIDIQVGRTGALTPVGKLEPVTVGGVVVANATLHNEDEIKRKDVRIGDTVI
                     VQRAGDVIPQIVGVVKEKRPKGAKEYVFPTRCPVCGSHAVREVNPRTGEMDAVRRCTG
                     GLICPAQAKERLRHFVSRNAFDIEGLGEKQIEAFYDEGRIRQPADIFKLARREEQRAD
                     KLIDKKGYGRKSLENLFKAIESRRSVPLDRFIFALGIRHVGETTARDLANAYHTWEAF
                     RAAVEAAVKGGHGSDAYRDIDNIEGIGETVVNALIDFFSEPHNVRALDDLLAEVTVQP
                     FTRVVSAKSSVVGKTVVFTGTLERMTRSEAKAQAERLGAKVAGSVSKKTDYVVAGADA
                     GSKLSKARELGVTVLTEDEWLELINQG"
     gene            11682..12398
                     /locus_tag="VF226_18900"
     CDS             11682..12398
                     /locus_tag="VF226_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX7076112.1"
                     /translation="MAGTHQSGLTAVSAWDEFRQGTIAVLPAAVAVVPFGLLLGALAA
                     QKGLSTLEIGLMSGLVFAGSAQMVAVDIWREPAPWVLLGVAAFTINLRHLMMGASLSF
                     HLGGFRTWQRILGVAFLADEIWAMAERRAATGQLHPAYYAGLAFLLYANFVWATVAGA
                     IIGPAIEDPTAWGFDFAFTAIFIGLIVAFWRGSGTALVVAASAVTATLTQIQFGGVWH
                     VLAGGAAGMLAAAVLAIIRE"
     gene            12406..12708
                     /locus_tag="VF226_18905"
     CDS             12406..12708
                     /locus_tag="VF226_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX7076113.1"
                     /translation="MTVDPLNLAAILAMAAATYATRIGGLWLLRFVHVTPRVRASLDA
                     LPVAVLTAVIAPSLMKGGPADMIAAAITILAASRLPLLAVVLIGVGSAVLLRHVLN"
     gene            complement(12722..14026)
                     /locus_tag="VF226_18910"
     CDS             complement(12722..14026)
                     /locus_tag="VF226_18910"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7076114.1"
                     /translation="MTQTLRRVAVLGGVRIPFCRSNTFYADLSNLEMMTGALNGLVEK
                     FRLQGVHIDEVVGGAVVTHSRDWNLAREAVLGTELAPSTPGITLMQACGTSLQAALMI
                     AGKIASGQIESGIAVGSDTTSDAPIVVSKRLAKRLTEMAQQKTVWDKINVFKGFSPAE
                     LIPQPPSVAEPQTGLSMGQHCELMAQEFQIPRAEQDRLAYESHKKAAEAYASGYMDDL
                     VVPFAGVFRDNNIREDIDLDKLATLRTVFDKSGRGTLTAANSTPLTDGAAAVLLASEE
                     WAASRGLPVLAWLTAYQTAANNFVEGEGLLMAPTIAVSKLLDRTGLKLQDFDFYEIHE
                     AFAAQVLATLKAWEDPEYCRRVLGKDQPLGSIDRSKLNVKGSSIAYGHPFAATGARIM
                     ANLAKLLYEHGGRGLISICTAGGMGVAAIMESARAAEMRAAA"
     gene            complement(14045..>15151)
                     /locus_tag="VF226_18915"
     CDS             complement(14045..>15151)
                     /locus_tag="VF226_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7076115.1"
                     /translation="MEMLKDQAPKDLGWKPLRVHVIGAGTMGADIAGWCVASGMEVSL
                     QDISPEQIKKGIEAQEKLFARKFKTKAQRDAAKARLIADPQGRNISRADVVIEAVVEK
                     LEVKQEVFKSIEPKLKPGAVLTTNTSSIMIEDIAQPLSDPGRLIGLHFFNPVSRMPLV
                     EVVRGEASREEELKKGAAFVTAIDKLPLIVKSVPGFLVNRVLAPYLTGAMQRLQAGEE
                     KEKIDEAARTFGMPMGPIELADTVGLDVCAHVGRILNFGAGGTELDRMVSQGKLGKKS
                     GEGFYVWKDGKPQKADKTYDKAELERLGRELVAPLIAECERCRDEGVVASADLIDAGV
                     IFGTGFAPFRGGPLHYKATEAAAEAGEQHRAAAE"
BASE COUNT         2694 a         4903 c         4772 g         2782 t
ORIGIN      
        1 agctccgtcg ccttctggac catgtagtcg agccgcgcgc gcttcagcgg cgcgaagagg
       61 taatggatgt ccgggccggc ctcctgctcg cgcacctgtt ccagcggccg aagggtgcag
      121 ctccgtttgc cttcgaccgc aagctgcgcc cgccactcgc catcccggcc gttgaagatc
      181 agtatctcgg cgcctgcccc caggcgcaag acgttgcgca gatagttcgc ctgctcggcg
      241 gtgcagggga cctccacgcc tgcgcggagc ccggcttcga caaagagacg ctgggcggtg
      301 aaatcgtgaa cggccatgat gcctgatagc gcacgaccag aacactttgt agcccgctca
      361 ctccgaaatc tatcctgcgc gtgacctcga gagtgtcgga ttctgttgac actcccctgg
      421 cacgctgatt gaccgatcca gaactcctgc caagttgcga cggatgaact cgacgaccga
      481 cacgccaaac gtcattgcag atgccccgcc cgacaattgg gttgacaggt atgcgccggt
      541 ggcgctgcag ccttacttga agctcggccg cttcgaccgt ccgatcgggt cgtggctgct
      601 gttgtttccg tgctggtggt cgctggcgct cgccgagctc tctcgcgggc ggccctatcc
      661 caacctctgg tacgtgctgc tgttcctgat cggcgccttc gtgatgcgcg gggcaggctg
      721 cacctggaac gacatcgtcg atcgcgattt cgacggcagg gtcgcccgca ctaggggacg
      781 tccgattccc tcgggccagg tgagcgtcac ccaggcgctg ctcttcggtg tcgcactgtc
      841 gctcgtcggt ctcgcggtcc tcgtccagtt caacgcgttc acgatctggc ttggcatcgc
      901 ctcgctgggg cttgtggcca tctacccctt tgccaagcgg ttcaccttct ggccgcaggt
      961 ggtgctggga ctgacattca agtggggcgc tctggtcgga tgggccgctg ttttcggcca
     1021 gctcagcgcg gcgccgatcg tgctttacgc tggatgcgtc ctctggacga tcggatacga
     1081 cacgatctac gcccatcagg acaaggagga cgacagtatc ctcggcctga agtcgacggc
     1141 cctgcggttc ggcgatgcga gccagaactg gattgccgcc ttctacgccg gggctctcgt
     1201 gctttgggca gctgccgctt ttctcgctgg cgcgcacctg atcttctata cggcgctggc
     1261 tctcgtcggc gtgcagctcg cctggcaggt tgcgagcctc gacatcgatg atcccgccaa
     1321 ctgtcttcgc cgcttccgtt ccaaccgcga tgtcggctgg gcgctgtttc ttggcctgat
     1381 cgccgacatg gccatttcgg cgctggcggg actcgcctga gcctcggagg cttcggccat
     1441 cttcgtcatc ccggcgtttg tcgccgggat ccagctatgc acgcgtcagg acctgcgaat
     1501 tgttgcttac gtcagcccgc tgatgagtgg accccgggca tgaagcccgg ggtgactgga
     1561 agtgggctgc tgctgtcgtt cgcgtcacgc cgccctgtcc atcgcctcga gctcgtcgat
     1621 catgcgctcg atgacgttga ggcccttctg ccagaactcc ggttgccgcg catcgaggcc
     1681 gaatggtgcg agcagctcgg aatggtgctt cgagcccccc gccttcagca tgtcgaagta
     1741 cttcgtcacg aagtcggggt gagcctcctc gtagagcgca tagagcgagt tcacgaggca
     1801 gtcgccgaac gcataggcgt agacgtagaa cggcgaatgg atgaagtggg ggatataggt
     1861 ccagaagacc tcgtagccag gcttcagctc gatggccggc ccaaggcttt cgccctgcac
     1921 ctccaaccag aattcgttga gccgatcggg cgtgagctcg cccttgcgcc gctcctcgtg
     1981 caccttccgc tcgaacgtgt agaacgcgat ctggcggacg accgtgttca gcatgtcctc
     2041 caccttggag gccatgagcg ccttgcgctc gcgcgcgtcc ttggcctcgg cgatgagcgc
     2101 ccggaaggtc agcatctcgc cgaaaaccga ggcggtctca gccagcgtca gcggcgtttg
     2161 cgacagcagc ggcccgaggg gagccgccat gacctggtgc acgccgtgac ccagctcgtg
     2221 agcgagcgtc atcacatcac gtgacttgcc ctggtagttg agcagcacat aggggtgggc
     2281 cgagggcact gtcggatggg cgaaggcgcc cggcgccttg ccgggccgga cgggcgcgtc
     2341 gatccagcca ttctcgaaga acttgcgcgc aatgctggcc atctctggag cgaagtcgtt
     2401 gtaagcggtc agaacgatct cttgcgcctc cgaccacggc acgatgcgct cgggcttttc
     2461 ggggagcggc gcattgcggt cccagtgagc gagcctgtct ttaccaagcc agcgcgcttt
     2521 catcttgtag tagcgatgcg acagcttcgg gtagctggcc cgcacggctg aaaccagcgc
     2581 atcgaccact ggcccctcga cgcggttcgc gagatggcgg gaatccgcca catccttgaa
     2641 attgcgccag cggtcggaga tctccttgtc cttggcgagc gtgttcgtga tcagcgtgaa
     2701 aagccgcagg ttttccttga aaaccttcgc cagggcctcg gcgcctgcgc ggcggcgccc
     2761 ctcgtcggcg ctggtcaaca gattgagcgt cggctcgagc gccagcggct ccggctcccc
     2821 ttcgaccgga aaacgcagcg cagccattgt ctcgttgaaa agacggttcc aggcgccgcg
     2881 cgatgtctgc gatttctcgt ggaagagctg ctccagcttt tcgtcgagct gatgcggctt
     2941 ctcctttcgc agatcgtcga accagggctt gtagcgcgcg aggtcagggt gcttcagcgc
     3001 ctcagcgaga tccgcgtcct cgatcttgtt gagctccagc tcgaaaaaga tgaggtcgtt
     3061 cgaaatattg gtcaggcgct cgctcgtgtc tccgaagaac ttggcgcgca ccgggtccga
     3121 ctgatcggcg gcatagagca gccccgcgta agacccgagc cggcccatga ggtcgccgag
     3181 ctgctcgtaa tccttcacgg ccctggcaag agccgcgcca tctcttccca ttgcgacgag
     3241 ccggccctgg taggtctcct tgatccgttt cgcctcggca gcggccctat cgaaatcccg
     3301 ctcgacctcg ggtgcgtcgg gagccgcgta gagatcactg agcttccatt ccggcatcgc
     3361 gccgagatcg acggccgcgc tcgccgagga cgctccggcc tgcgccgcct gcttcggcca
     3421 gctgttcgag atggagtgca cgaaggtcat tcacgggctc ccgttgttct gtattccaag
     3481 tcaggtggga cttaggacct aatgtgcgag cctggggcag cgggtgcaag gaaaccctgc
     3541 atcccgggct ccacacgcct gggccactga cccggcgagg ctcagcatgg ttccagcagc
     3601 aaccagttat ttacgcctgc tgtgcactga tgcgagagcc ggttgggcgc tggtgtgcct
     3661 gcccggcctg ttgagttgcg tccttgttcg agttgatcat ggctaagcgc gtattgatcg
     3721 tcgatgacga tccggcccag cgccgtattc ttgaagagtg catcaagcgg ttcggattcg
     3781 agtgcaaaac tgcagcttcg ggcgatcagg ccctgcatat tctcgccggc cctgaaaagg
     3841 acggcatcgc tatcgttctc ctcgatctgg tgatgcctgg cacgaacggc atgacggttc
     3901 tggagcagct ccgcggcaag ccaggcattc cgcccatcat cgtccagacg tcgcacggtt
     3961 ccattgacac tgcgatcagc gccatgcggg ccggcgcggt cgacttcgtc gtgaagcccg
     4021 ccagccccga gcggctggaa gtttcgatca acagcgcgct gaagctccag gccctcagcg
     4081 gtgagatcgc ccggctcaag aagaagagcg aaggcacgct gacgttcgcc gacctcatcg
     4141 ccggtcaggc gatgcagcgg gtcgtctcgc tcggcaagcg cgccgccggg tcgcagatcc
     4201 cagtgctcat cgaaggggaa tccggtgtcg gcaaagagct catcgcgcgc gcgatccagg
     4261 gcgaaagcga ccgcgccggc aagccgttcg tgaccgtcaa ctgcggcgcc attccggaga
     4321 atctggtcga gagcatccta ttcggccacg agaagggcgc tttcacggga gccgtcgaca
     4381 agcgcatcgg caagtttcag gaagctgacg gcggcacgct gttcctcgat gaggtcggtg
     4441 agctgcccct cgaagcgcag gtgaagctgc tgcgggcgct gcaggatggt gaaatcgatc
     4501 cggtcggcgc caagcggccg gtgaaagtgg acatccggct catctcggca acgaaccgcg
     4561 acatgatcga gctggtgaag tccggccggt tccgtgagga cctgttctac aggctcaacg
     4621 tcttcccgat ctgggtgccg ccgctgcgcc agcgattgga cgatgtgccg gagcttgccc
     4681 atcactttct cgcgaggttt gccgccgagg aaggcaagca cgtcacctcg atcagcgagc
     4741 aagccatgca gctgctgacc cgctactcgt ggccgggcaa tgtgcgccag ctcgagaacg
     4801 cggtgttccg tgccgtggtc ctcgcggacg gcccagagct gacggttgcg gaattccctc
     4861 agatcgccat gcacgtcgag ggcttctcgg cggagattcc cgctgccccg ccgccgcaga
     4921 aaccggcggt ctacaccggg ccggcgatga tcggcgctga tgacaaggtg cctcgcacga
     4981 tcgagttgaa ggggactgac ggcggcaagg cgatcggcat tcctgccgtc acggaatcag
     5041 gcgacgtgcg cacgctggag gccatcgagg ccgacatgat tcggctggct ctcgggcgct
     5101 acaggggcca catgtcggag gttgcaaggc gactgggtat cggccggtcc acgctctatc
     5161 ggaagatgca ggagtatggg ctcgagtcgc gccccaactg agatctgatt gtcggagacc
     5221 agagcgaccg gcgagcagcc gattgctgag ctgccttgcc atttcggcca cagttccgac
     5281 aaaagaagat tctcaggaaa gatcagcctt gtaactgttg ctggctgcca cgtggcgcat
     5341 taggtaacgt gccaagccgc atggagaatg aatgatgcga cctgcacgga cactgctcac
     5401 cgggctagcc ctttccactc tcctcgccag cgcagcgctg gcatccggcg agccggatcc
     5461 gggggaagcg agcccggcgc cggcagccgt ggctgagccg gtgtccggca attcggaggc
     5521 cgtgtccgcc accgatgccg cggctgcgcc ggtcgcggag acgagcaatt ctgcggaaca
     5581 ggcagaagac actgccgatc cggccgggcc gagcgctgca tctgggcagc agaccggatc
     5641 ggacgtgacc gccaggcatg atgccgatga ggctgggtca tcacagaccg ccgatgttca
     5701 acccgcagcc tcgggcgcag cgagcgaggc cgagcccgat gcctcgatcg agccagctac
     5761 tgcagctgct tccgaacccg agaagccagt cgtggagcag gatggctcga ccgtaagcac
     5821 tgcctccacc acgactgccg atccggttgc gggcgaagcg gcggaggagc aacctgagca
     5881 ggcgaccgct gggcaagagc cggccgctca agcttcagac tccctcgcca tcacggatcc
     5941 aggcgtggac gatcaggcgg agcctgaacc cgaacaggcc tcaacgccgg atgcgcccac
     6001 tgaaccccaa tcaccagcat ccgctgaagc tgatccaacc aatgaagcgc ctgaaacggc
     6061 cccggccgct gcagcagcgc agtccgggca gcctgagcaa tcgtcggata ctgcatccgc
     6121 tgagcagacc atcgaggtca gccagttcga gccggagctt ctgcggctga tttccgaagc
     6181 ccaagacctc tcggatgccg agcgtgaggc gctgaccgag ttctacaagg ggcgcggcga
     6241 cgatttcctt tgggtcgctg atgggacgtt caccccgcgg gccgaagctg ccatggcgga
     6301 aatccgccgc gctgacgact ggggtttgga ggcatccgcc tttgagctac cggatgccgt
     6361 ggagtccagc gagccggccg atgtcgcggc tgccgagctg aagctttccc tggccgtcct
     6421 gaaatatgcc aagcacgcgc gcggcgggcg gatggatccc cggcagctct cgaactacat
     6481 cgatcgggag ccgccactgc tgccgcctgc cgaagtgctg gccggcatcg ctgcagccga
     6541 tgctgcggac ggatatctgc gcggcctgca cccgcagcat ccgcagttcg agaagcttcg
     6601 ccaggcgtgg ctcgacctca ggaatggcaa gctgccggcc gaggatgccg aagaggatgt
     6661 gacctccaaa agccgtgcca aaaggaagag ccgtgagacg aactccgctg aaagcaagat
     6721 gcgcaagctc ctgtacaaca tggagcagtg gcgctggatg ccggaggacc tcggccagtt
     6781 ctatgtctgg gccaatattc ctgaattcac actacgcgtc gtgaaggatg ggaaaatcgt
     6841 cttcacggag cggctggtcg tcggcaaagc cgatacgcag actcccatct tctccgacga
     6901 gatggagacg atcgtcttcc atccattctg gggcgtgccg aacagcatca aggtgaagga
     6961 gatcctccct ggtctgatac gcggcaccaa cgtgcttgcc cgcaacgatc tgcggattca
     7021 gtaccgcggc cgggacatcg acccatggag cgtcgattgg acccgcacgg atatccggaa
     7081 cttccacgtc taccagccgc ccggcggcaa gaacgtgctt ggcgtggtga aattcctgtt
     7141 cccgaacaag cacgccgtct acatgcatga cacgccgacg aagaatctct tcagcgcctc
     7201 gcagcgcacg ttcagccacg gctgcatgcg tgttcgcaat ccgctgaaat tcgccgaggt
     7261 cctgctagct tacgacaagg gctggagcga gggccgcgtt ctgtctctgg tgagaaacgg
     7321 ccccagaaac aatcacgtga acgtcgagag caagatcccg gttcacatca cctacttcac
     7381 ggcgtgggta gacgaagacg ggaaggttag aacctggcct gatgtgtatg gccacgagaa
     7441 gcgcatccag atgggcctgg agggcaaagc gcatctcatc gccaagacca aggaggatct
     7501 cggcgccgtg caggctgagg tcgtcgggcg gttggcagag acgcggccca cctggtcctc
     7561 gccctcgtac tcatcttctc gtcagccgga ctgggcacgc cgcgccttcg gccactgagc
     7621 gcgccaaccc ggttaataaa atcgtcactt gaataaagcc ccgcgtgatg ccgagcgggg
     7681 ctttattttt gcccgcttcc gagctccaac cggtcgctca tttagttcga tcacgccctt
     7741 ctgcctcttt gtcgcccgag tgcaccttta ggagcacctc tgaggggaga cttaacggga
     7801 ggttaacaat ccagcgttaa cgctaagaag cggcccgaag ccgtgtcaca ccgggacatt
     7861 gccggcaggc ttgagaggca gtcagcgttc gatcaggggt ggggctagca ttgtataagc
     7921 gtgggctgag tctcctcgca ggtgtcgccg cattggtatt tgttgcggtt tacggagcac
     7981 cgcggctaac tgccggactg ccgcaaacgc ggactatttc gctgtacaac atccatacga
     8041 aggagaccat cacggtcgag tacaagaagg acggcaagta cgtcccggcc gcgatggaaa
     8101 agatcaactg gatcctgcgc gactggcgta agaacgaagc gacccagatg gatccggcgc
     8161 tcatcgatct gctctgggaa attcacaccg agctcggctc ggaagagccc atccacatca
     8221 tctccggcta tcgctcgcgc gcgacgaacg acatgctgcg gaaaacccgg ggcggacagg
     8281 ccaagcagag ccgtcacatc ctcggcaagg ccgccgatgt ccatttcccg gatgtgccgc
     8341 tccgccgctt gcggtattcg gcgctcgtgc gggagcgcgg cggtgtcggc tattatccga
     8401 cctcggctat tccgttcgtt cacgtcgata ccgaccgggt ccgcgcatgg ccccgtttgc
     8461 cgcgctacga gctggcgctg ctcttcccgg acggccgaac caagcatatc cctgccgacg
     8521 gacggccgct gacgccggag gacgtgcacg aggcgcggaa gcaccattct gaattggcgg
     8581 cccaggtcgc cgcctatcag gagctgcgca aggcagcact gagcccgacc cggatcgcaa
     8641 aggcggaacc agtcgcccga gatatacctc gcccgggatc gcggatcgca gcagcctggc
     8701 aacagacgga gccgcagcag ccatcgcatg agccagcgaa cgctcaggaa aagccattgc
     8761 ccaagctcgt tgccggtccg cgcccggcaa tccggcctcc cagggtcgcc aatcaaccgt
     8821 ccgacgagga ccgcatgaag ctggcgcagc tggcggcgct tgcgagcctg tcaagcgttc
     8881 cctcgggggc gcggctcatg caggcctcgt tgaaagctga tgagcccttg ccggaagagc
     8941 gcctgacggg atcggtgcca ggtactgatc aatcctcgaa tacccgcttg gcggctctag
     9001 atttcgaagc gggcggtcaa cccgctcctg gtcgtttcca ctggggcgtc ggctggacga
     9061 cagagcgcaa agaggagatc gagcccacca cgcctactgg atgggccgtc gcaccggaat
     9121 acgatgagga gcatcccgag gagctgtcct accggccatt ccccataatc ccatatctga
     9181 cggaaacgcc gtctccggac gatcctgcgc ttgcgcgcat ggagcacccg gatgtggcca
     9241 agacgctcga gcttctggac aacgccggtg acctgcccgt gatgcagctg agggccgagc
     9301 cgcagacggc acagctgctg tgggcgcagc agtttcaggg cgaggccgtg ccgctcgcca
     9361 gcgtattcgg cgcgaggcca cccgcggccc ccagcgggct caacgagcgc cacgtgcgtc
     9421 tcagcgcacg ctgagcgcga ctctggcgag caaagccgga caggttctca tgctcgcccg
     9481 cgccccacag gccccgatca ctgttccaac cttacgcttg atccaacaga acgcgcgtgc
     9541 cacctaacgc agggttccct tttgttgtgg cccgctctca tgacgacttc ggctgtctcc
     9601 cataagcctg tttcggcgct gacccccaag gaagcagagg aggagcttgc ccggctggcc
     9661 gccgagatag cgcaccacga tcggctctac tatcagctcg cgcagccgga aatctcagat
     9721 gccgaatacg atgccctggt ccgccgcaac gccgagatcg agacgcgttt tcccgagctg
     9781 atccggcccg acagtccgtc ccgccgtgtc ggggcgccgc cggtcgaggc cttcggcaag
     9841 gtccggcatc gcgtgccgat gctgtcgctt tcgaacgcct tctctgaaga ggaaatcatc
     9901 gagttcgtcg atcgtgtgcg ccgtttcctc ggcatgaagc ccagcgagac gatcgagttc
     9961 acggccgagc cgaagatcga cggcctgtcc atctcgatcc gctacgagca cgggcggctg
    10021 gtcgaggccg ccacccgggg cgacggcacc gagggcgaaa acgtcacggc caatgtccgc
    10081 accgtccgag acattcctca tcggatcacc gggcccggat gccccgacat catcgatgtt
    10141 cgtggcgaga tctatctggc gcacgccgat ttccagaagc tcaacgaggc acaggcagcc
    10201 agcggccaga agattttcgc gaatccgcga aacgcggcgg ccggctcgct ccgccagctc
    10261 gacccgaagg tgacggcgag ccggccgctc aagttcttcg cctatgcctg gggggaggtc
    10321 tcacaccttc ccgcgcgcac gcagtgggag atgtacgagg tctatcggcg ctggggcttc
    10381 ccgaccaatc cgctggcccg gctgtgccgc gggccagagg agatgctcgc cttctaccgg
    10441 gagatgagca cccggcgggc caccctcggt tatgacatcg atggcatcgt ctacaaggtg
    10501 aaccggctcg acctgcagga tcgcctcggc ttcgtatcgc gctctccgcg ctgggccgtc
    10561 gctcacaagt ttccggccga gcaggccatg accatcctgc gtgacatcga catccaggtc
    10621 ggccgcaccg gagcgctgac acctgtcggc aagctcgagc cggtgaccgt cggcggcgtg
    10681 gtggtggcaa acgccacgct ccacaacgag gacgagatca agcgcaagga cgtgcgcatc
    10741 ggcgacaccg tcatcgtcca gcgcgcaggc gacgtcatcc cgcaaatcgt cggggtggtg
    10801 aaagagaaac ggcccaaagg ggcgaaggag tacgtgttcc ccacgcgctg cccggtttgc
    10861 ggcagccatg cggtgcgcga ggtcaacccg cgaacaggcg agatggacgc ggtgcgccgc
    10921 tgcacgggcg gcctcatctg cccagcgcag gcgaaagagc ggctccgcca cttcgtctcg
    10981 cgtaacgcgt tcgacatcga gggattgggc gagaagcaga tcgaggcttt ctatgatgaa
    11041 ggccggatca gacagcctgc ggacattttc aaactggcgc ggcgagagga gcagcgcgcc
    11101 gacaagctca tcgacaagaa gggttacggc cgcaagtccc tcgaaaacct gttcaaggcc
    11161 atcgagtcgc ggcgcagcgt gccgctcgac cgcttcatct tcgcgctcgg cataaggcac
    11221 gtcggcgaga cgacggcgcg cgacctcgcc aacgcctatc acacatggga ggccttccgc
    11281 gccgctgtcg aggcggcggt gaaaggcggt cacggcagcg atgcctaccg cgatatcgac
    11341 aacatcgagg gcatcggcga aaccgtcgtc aacgcgctga tcgacttctt ctccgagccg
    11401 cacaacgtgc gcgccctcga tgacctgctt gcggaagtga cggtccagcc attcacccgc
    11461 gtggtgtcgg ccaagtcgtc cgtggtcggc aaaaccgtcg ttttcaccgg cacgctcgag
    11521 cgcatgacgc gcagtgaagc aaaggcgcag gctgaaaggc taggggccaa ggtggcgggc
    11581 tcggtctcca agaagacgga ctatgtggtc gcgggcgcag atgccggctc caagctgtcg
    11641 aaggcgcgcg agctcggagt cactgtgctg actgaggacg aatggctgga actcatcaat
    11701 cagggctgac agccgtctca gcctgggacg aattccggca ggggacgatt gcggtgttgc
    11761 ctgcggcggt cgctgtcgtg ccgttcgggc tgctcctggg cgcgcttgct gcgcagaaag
    11821 ggctgtccac gctcgagatc ggcttgatga gcggcctcgt atttgccggc agcgcgcaga
    11881 tggtggcggt cgatatctgg cgcgaacctg caccctgggt gctgctcggc gttgcggcct
    11941 tcacgatcaa tttgcgtcat ctcatgatgg gcgcgtcgct cagttttcat ctcggcggtt
    12001 tccgcacctg gcagcgcatt ctcggcgtcg cattccttgc tgatgagatc tgggccatgg
    12061 ccgagcgccg ggcggcaacg ggacagctcc atccggccta ttacgccggg cttgccttcc
    12121 tgctttatgc gaacttcgtc tgggcgacgg tcgccggcgc aattatcggt ccggcgatcg
    12181 aggaccccac ggcctggggg tttgattttg cgttcacggc aatcttcatc ggcctcatcg
    12241 tcgccttctg gcgcgggtcg ggcacagctc tcgttgtcgc cgccagcgcg gtcacggcga
    12301 ccctgactca gatccaattc ggcggggtct ggcacgtact ggccggcggc gcagccggca
    12361 tgctggcggc ggctgtgctg gcgatcatcc gcgaatgagg gccttatgac ggtcgatccg
    12421 ctaaacctcg ccgccatcct tgccatggcc gccgcgacat acgcgactcg catcgggggg
    12481 ctctggttgc tccgcttcgt ccacgtcacc ccgcgcgtgc gggcctcact cgatgcgctg
    12541 cctgtggcgg tgctgaccgc cgtgatcgca ccctcgctga tgaagggcgg gcctgccgac
    12601 atgatcgcgg cggccataac gatccttgcc gcctcgcgcc tgccgttgct cgccgtcgtg
    12661 ctgatcggcg tcgggtcagc cgtgctgctg cggcacgtct tgaactgaaa cgcgctgcgg
    12721 cttacgccgc cgcccgcatt tctgccgccc gtgcgctctc catgatggcc gcgacgccca
    12781 tgccgcccgc cgtgcaaatg gagatgaggc cgcgcccgcc atgctcgtag agcagctttg
    12841 cgaggttggc catgatgcgc gcgcccgtgg ccgcgaatgg atggccgtag gcgatcgagg
    12901 agcctttcac gttcagcttc gagcgatcga tcgatccgag cggctgatcc ttgccaagca
    12961 cacggcggca atattcggga tcttcccacg ccttcagcgt ggcgagcacc tgggccgcga
    13021 aggcttcgtg gatctcgtag aaatcgaaat cctgcagctt gaggccagtg cgatcgagca
    13081 gcttggacac tgcgatcgtc ggcgccatca acaaaccttc gccctcgacg aaattgttgg
    13141 cagctgtctg ataggccgtc agccaggcga gcaccggcag tccgcgtgaa gccgcccatt
    13201 cctcggacgc gagcagcaca gcagcggcac catcggtgag cggcgtcgaa ttggcagccg
    13261 tcagagttcc gcgtccggat ttgtcgaaaa ccgtgcgcag tgtcgccagt ttatcgaggt
    13321 cgatgtcctc gcggatgttg ttgtcgcgga acacgcccgc gaatggcacg acgagatcgt
    13381 ccatgtaacc ggaagcgtag gcttcggcgg ccttcttgtg gctttcgtag gcaagccggt
    13441 cctgctccgc gcgcggaatc tggaattcct gcgccatcag ctcgcaatgc tggcccatgg
    13501 agaggcccgt ctgcggctca gcgacggaag gcggctgcgg aatcagctca gcgggcgaaa
    13561 accctttgaa cacattgatc ttgtcccaca ccgtcttctg ctgcgccatt tccgtgaggc
    13621 gcttggcgag ccgcttcgac acgacaatcg gcgcgtcgga cgtcgtatcc gaacccacgg
    13681 caatgccgct ctcgatctgc cccgacgcga ttttgccggc gatcatcagg gctgcttgga
    13741 ggctcgtgcc gcacgcctgc atcaaggtga tgcctggggt tgagggagca agctcggtgc
    13801 cgagcaccgc ctcgcgcgcg aggttccaat cgcgcgagtg cgtgacgacc gcgccgccga
    13861 ccacctcgtc gatatgcacg ccctgcaggc ggaatttctc caccaagcca ttcagcgcac
    13921 cggtcatcat ctcgaggttc gagagatcgg cgtaaaaggt attcgagcgg cagaagggaa
    13981 tccgcactcc gccgagcacg gctacacgtc gaagcgtttg ggtcatcctt ttcctccgcc
    14041 ctctttattc tgccgccgcg cggtgttgtt cgccggcttc ggccgcagcc tctgtcgcct
    14101 tgtaatgcag cggcccgccg cggaatggcg cgaacccagt gccgaaaatg acgcccgcat
    14161 cgatcagatc ggcgctcgca accacgcctt cgtcgcggca gcgctcacat tctgcaatca
    14221 gtggagccac cagctcgcgg ccaaggcgct ccaattcggc cttgtcatag gtcttgtccg
    14281 ctttctgcgg cttgccgtcc ttccacacgt agaagccttc gccggatttc ttgccgagct
    14341 tgccctgact caccatccgg tcgagctcag tgccgcccgc gccgaaattc aggatgcgcc
    14401 ccacatgggc gcacacgtcg aggccaaccg tatcggccaa ctcgatcggc cccatcggca
    14461 tgccgaaagt gcgcgcggct tcgtcgatct tttccttttc ctcgcccgcc tgaagccgct
    14521 gcatggcgcc ggtgagatac ggcgccagca cgcggttcac gaggaaaccc ggtacgctct
    14581 tcacgatcag cgggagcttg tcgatggccg tgacaaacgc cgcgcccttt ttcagttcct
    14641 cctcgcggct cgcctcgccg cgcaccactt cgaccagcgg catgcgcgac accggattga
    14701 agaaatgcag gccgatcagc cggcccggat cgctcagcgg ttgtgcaata tcctcgatca
    14761 tgattgagga ggtgttcgtt gtgagcacgg cacccggctt gagcttgggc tcaatggatt
    14821 tgaagacctc ctgcttgacc tcgagttttt cgacgacggc ttcaatcacc acgtccgccc
    14881 ggctgatgtt gcgcccctgc gggtccgcga tgaggcgcgc cttagccgcg tcccgttgcg
    14941 ctttcgtctt gaacttgcgg gcaaacagct tctcctgggc ttcaatgccc ttcttgatct
    15001 gctccggtga aatgtcctgc agcgacacct ccatgccgga agccacgcac cagcccgcga
    15061 tatccgcgcc catggtgccc gcgccaatca catgcacgcg cagcggcttc cagccgagat
    15121 ccttcggcgc ctgatctttc agcatctcca t
//
LOCUS       DASVDU010000283        10739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_585091, whole genome shotgun sequence.
ACCESSION   DASVDU010000283 DASVDU010000000
VERSION     DASVDU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585091"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VF226_18920"
     CDS             complement(<1..867)
                     /locus_tag="VF226_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX7076116.1"
                     /translation="MKEDLFPLSIARTLHHNNSNIELIEASIARGESRFASTGALVAE
                     TGSHTGRAAQDKFMVRDERTEKAVWWDANKAMSREKFDVLHRDFLEHAKGRELFVQDL
                     FAGAEPASRLNVRVVCEYAWHALFIHYLLRRPVASELAGFEQGLTVVCLPSFRADPER
                     HGCRTETVIACDFTRGLVLIGGTSYAGEMKKSVFSFLNFVLPERGVMPMHCSANAGPN
                     GDAAIFFGLSGTGKTTLSADVKRTLLGDDEHGWSSEGIFNFEGGCYAKTIRLSPEAEP
                     AIWAASNRFGSLL"
     gene            complement(1199..1936)
                     /locus_tag="VF226_18925"
     CDS             complement(1199..1936)
                     /locus_tag="VF226_18925"
                     /inference="COORDINATES: protein motif:HMM:NF025252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7076117.1"
                     /translation="MPNAGANPFPHQDLAPATAVRHLLRRARKAALSTIDRQSGYPYA
                     SLVAMATEPDGAPILLLSKLALHTKNIEGDARASLLIEEGSGRGDPLTGSRVTLTGRV
                     QPALSNTARRRFLAVHPTAAGYAHFADFSFFVLHLERAHFVGGFGRIFPLDRGDILIG
                     TSATEDLVSTEPELIASINRDEAERIGRLAERLCGGRSGAWRLTGIDPHGCDLALGAE
                     RLRLDFPEPATTADTARRMLSDILERE"
     gene            2101..2808
                     /locus_tag="VF226_18930"
     CDS             2101..2808
                     /locus_tag="VF226_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7076118.1"
                     /translation="MKEKSTIALVDDERNILTSLRMALEAEGYKVRTYSDGASALEAL
                     TEDPADLGIFDIKMPRMDGMELLRRVRQQSDMPVIFLTSKDEEIDELFGLKMGADDYI
                     SKPFSQRLVVERVKAVLRRFQPKDGSNSQSETARVIERGQLRLDPERHTCHWDGKPVT
                     LTVTEFLILQALAARPGVVKTRDALMDAAYDDQVYVDDRTIDSHIKRLRKKFKQVDEN
                     FDVIETLYGVGYRFKEV"
     gene            2823..4679
                     /locus_tag="VF226_18935"
     CDS             2823..4679
                     /locus_tag="VF226_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEX7076119.1"
                     /translation="MAIELERSGPIAGPWRAARESFLRVHRAARPMREFLAAVGNRLL
                     RSRAFRLVTGSLLRRIFVSNLLGLLILVGGILYLSKHNVWLIDAKRESLRSQSEIIAA
                     AIAANATVDTERIVLDPDKLPEAEDALIPFRDDGFAALQLSIRPERVTPILRRLVQPT
                     NNRARIYSRDGTLIADSAQLLSRGLIAKPPPPKARPDTKNFWTRLTKWLLNADLPVYK
                     EIGNANGNFYPEVRMAMTGTTTPMLLLNEDGEQIVSVAAPIQRARSVQGVLLLSSKPG
                     EIDQALAQERWTILKIALMALAATLAASILLARTVAGPMRRLSEAAEHVSRNISARHD
                     LPSYGDRSDEVGQMATAFRNMTTALIRRVEASEKFAADVAHELKNPLTAARSTAEAIA
                     YARDDAERRQLISQMQDELKRLNRLITDVSNASRLDAELALQQTEPVALCKLLDGIVN
                     TFSDILSGTGKSIAFEVDPRCSEDSLVVIGHEGRLSQVITNLVDNAISFSPEGGTVLV
                     RAGRFGNLIEIRVEDDGPGIPPENLEKIFRRFYTYRPTAESSRGNNSGLGLSISREIV
                     TAHGGEIWAENRMADGAGPDDRPLGARFVIQLPAKPVSGPLARSFGRGHRRG"
     gene            4660..5136
                     /locus_tag="VF226_18940"
     CDS             4660..5136
                     /locus_tag="VF226_18940"
                     /inference="COORDINATES: protein motif:HMM:NF019118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7076120.1"
                     /translation="MATAGARLIHGTAIALAGRAALIRGAPGSGKSDLALRCLAQASS
                     DLISGQALLVADDQVYAEVLDGKVQLGCPEPIRGLIEVRGIGIVAVPAQGSAVLELLV
                     DLVPRHEVERLPDPEAAAELFGIRIRRLGLYPFDASAPLKLLLALQGKGAITNGSQ"
     gene            5281..5685
                     /locus_tag="VF226_18945"
     CDS             5281..5685
                     /locus_tag="VF226_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7076121.1"
                     /translation="MIGLLIVTHGGLAGEFRAALEHVVGPQEQIETISIGPDDDMEAR
                     RQEILTAARRLDTGEGVILLTDMFGGTPSNLSISILDEANVEVIAGVNLPMLVRLARV
                     RSDTPIEKAVDLAKEAGRKYISVARQVLSGEP"
     gene            5701..5997
                     /locus_tag="VF226_18950"
     CDS             5701..5997
                     /locus_tag="VF226_18950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX7076122.1"
                     /translation="MLDRRKPSAVVTIRNRKGLHARASAKFVKCAESFNATVSVTRDG
                     NTVGGTSIMGLMMLAAGPGSTIHLEAEGPEGPEALEALVSLIEDGFGEDCVDEC"
     gene            6239..7642
                     /gene="ahcY"
                     /locus_tag="VF226_18955"
     CDS             6239..7642
                     /gene="ahcY"
                     /locus_tag="VF226_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7076123.1"
                     /translation="MPIAREYAVKDIGLAEWGRKEISIAETEMPGLMATRAEFGPGQP
                     LKGARIAGSLHMTIQTAVLIETLVALGAEVRWASCNIYSTQDHAAAAIAAAGVPVFAI
                     KGESLEDYWNYTRKIMEWGDGGTPNMILDDGGDATLLIHLGVRAENGDTAFLDRPGSE
                     EEEILFALIKRTLQEKKGWFNEVARNIRGVSEETTTGVHRLYEMQKKGTLLFPAINVN
                     DSVTKSKFDNLYGCRESLVDGIRRGTDVMMAGKVAMVCGFGDVGKGSAASLRNAGCRV
                     MVSEVDPICALQAAMEGYEVVTMEDAAPRADIFVTATGNRDVITIDHMRAMKDRAIVC
                     NIGHFDNEIQVAALRNFKWNNIKPQVDEIEFPDGKRIILLSEGRLVNLGNATGHPSFV
                     MSASFTNQTLAQIELFTNPGKYERKVYTLPKHLDEKVAMLHLAKVGAKLTKLTPAQAE
                     YIGVSPEGPFKSDHYRY"
     gene            7773..8726
                     /locus_tag="VF226_18960"
     CDS             7773..8726
                     /locus_tag="VF226_18960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX7076124.1"
                     /translation="MTLGRRFTLGLAAIATAAFTATAVAYPTKSIDYIIAFGPGGESD
                     ITARLQQPFFKQKFGQDLVVSYKPGGGGAVAWAELNSHPGDGHTIVGVNLPHIVLQPA
                     QKDVGYKTEDLTVVYWFHFTPDAIVVRADSPYKTLQDLIDDAKKNPGKVTFSGSGKGT
                     ANHLAQVRFDKMAGIKTTYVAFAGTGPSNTALLGGQTSAAWGYTTSAAAIGDQARILA
                     VAMEERHPKYPDVPTFKELGFDLVSGAYRGIAVPKSTPEDIRKKLSDMIGEINADPEF
                     KKKMLDGGYAMLDVPYEKNAEFMATQSKAFIEAAKEAGILK"
     gene            8819..10327
                     /locus_tag="VF226_18965"
     CDS             8819..10327
                     /locus_tag="VF226_18965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7076125.1"
                     /translation="MSGLETLLAALTPINLLLALAGVIAGTAIGAIPGLTATMAVAVL
                     VPFTFGMPPAPALILMGAIYTGAIYGGAYSAILLNTPGTPSAIATTLDGYPMARRGDG
                     DLAMTLACLASVFGGLIGALALLSLAPPLSRVALAFGPVEYFWLAIFGLSLISALSEG
                     SLLKGLAGGAFGLLISCVGVAEVSADVRLTFGSQTLIGGINVIAALIGLFCIPVLIDL
                     VATPGRHLNVVAEPRGFRLAEAVRVLIRKKFNLIRSSVIGTIVGILPGAGGSVAGIVA
                     YSEARRAARPEEEYGKGEPSGVIASESANNATVGGGFIPTLVLGIPGTPPDAVILGAL
                     LVQGLRTGPSLFTVHADIIYTFIWGLLIATILMLPIGLLLGRYAYKSIITLPKALLVP
                     SVAFMTMIGTYAIRNNISDVVIMVVLGVIGWILGRFGFAAAPIVLGLILGPIAEQGFV
                     QAWTIGSAQEDLVGMFFGRPISIAIIFFTIFSLASPILMRRWKVKPEAVHVE"
     gene            10317..>10739
                     /locus_tag="VF226_18970"
     CDS             10317..>10739
                     /locus_tag="VF226_18970"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7076126.1"
                     /translation="MSNERMPAQSADVGTAIIASLLIIFGAAAIWDTFSYADFDSAVF
                     PRMVAGVLILIAVGVLVRWVAQRIGAIAMTPTEVEESGGSWPRRILLVVVMLAAALAM
                     PWLGFLVAAIISFAALLLIAMHDPWTPSRIVVYPLVGLA"
BASE COUNT         1999 a         3443 c         3378 g         1919 t
ORIGIN      
        1 cagcagagag ccgaagcggt tcgaagcggc ccaaatcgca ggctcggcct ccggcgacag
       61 ccggatcgtc ttggcatagc agccgccctc gaaattgaag atgccttcgc tcgaccagcc
      121 atgctcgtca tcgccgagca gcgtgcgctt gacatcggcc gacagagtcg tcttgcccgt
      181 gccggagagg ccgaagaaga tggccgcgtc cccgttcggc cccgcgttgg ccgagcagtg
      241 catcggcatg acgccgcgct ccggcagcac gaagttgagg aacgagaaca ccgatttctt
      301 catctcgccc gcatacgagg tgccaccgat cagcaccaga ccccgggtga aatcgcatgc
      361 gatcaccgtc tccgtgcggc agccgtggcg ctccggatcg gcgcggaagc ttggcaggca
      421 taccaccgtc aacccctgct cgaaccctgc gagctcgctc gcaacgggcc gcctcagcag
      481 gtagtggatg aacaacgcgt gccaagcgta ctcgcagaca acgcgaacgt tgagacggct
      541 cgccggctcc gcccccgcga acaggtcttg cacgaagagc tcgcgcccct tggcatgctc
      601 caggaagtcc ctgtggagca cgtcgaactt ttcgcgcgac atcgccttgt tcgcatccca
      661 ccagaccgct ttttcggtgc gctcatcgcg taccatgaac ttgtcctggg cagcgcggcc
      721 cgtatgcgaa cccgtttccg ccaccagggc gccggtcgaa gcgaaccggc tttcaccccg
      781 cgcgatggat gcctcgatca gctcgatgtt ggagttgttg tggtgcagcg tacgcgcgat
      841 cgagagcgga aacaggtctt ctttcatttt catccttcaa ccgtccgttg gaggggccaa
      901 ggtccttgct tcgagttccg aacatccagg atagcagcca tcgatcacga aacttgggca
      961 gggtgcatcc gggtcgacac tcgcaggaac gagcggcgcc cgagcttggc ctcaagccgc
     1021 gtcaacgtgg ctttcaaggt tagggcataa accggccaaa gggtgaagat ctgtcgtata
     1081 cgttgacgcc ctggacttgc gccaagtcaa gccaatcgca gtgtcgacgg gctctgcggg
     1141 acggatcgcg ccggcgtcag gcctcgctgt tcgccaaggc cgctgccagg aacccggatc
     1201 actcccgttc gaggatgtcg ctcaacatgc gccgggccgt gtccgcggtg gtggcgggct
     1261 ccgggaaatc caggcggagg cgctccgcgc cgagtgcgag atcgcacccg tgcgggtcga
     1321 tcccggtgag acgccaggcg ccgctcctgc cgccgcacag acgttcggcc aatcggccaa
     1381 tccgctcggc ctcgtcgcga ttgatgcttg cgatcagctc cggctcggtc gagacgagat
     1441 cttccgtagc gcttgtcccg atcaggatgt caccgcggtc cagcggaaaa atccgcccga
     1501 agccgccgac gaagtgcgcg cgctcgagat gcaatacgaa gaagctgaaa tctgcgaaat
     1561 gggcgtatcc cgccgcggtc gggtgcacgg caagaaagcg ccgtcgggcc gtgttgctca
     1621 aggccggctg gacgcggccg gtcagcgtca cgcggcttcc ggtaagggga tcgcctcgtc
     1681 cgctgccctc ctcgatcagc aggcttgccc gcgcatcacc ctcgatattc ttggtatgca
     1741 gcgccagctt cgaaagcagc agaatgggtg cgccatcagg ttcagttgcc atcgcaacca
     1801 atgacgcgta tggatacccg gattggcggt cgatcgttga aagggccgcc tttcgcgcgc
     1861 ggcgcagtag gtgccgcacc gcagtagcgg gggcaagatc ctgatgggga aacggattcg
     1921 cgccagcatt cggcatatgc acaaccccag attgttgtca gcgaaaacct tgcctctaaa
     1981 ggcctactgg cctattcagc aataagttaa tcaaggggcc gcacctgaac gcgcgccaca
     2041 tttctcacaa gaacgggaca tagagcccgc aagcagcgac gaccgtccag ggcaagagac
     2101 atgaaagaaa agagtacgat tgctctcgtc gacgacgagc gaaacattct gacctcgctc
     2161 aggatggctc tcgaagccga aggctacaag gtgcggacgt acagcgacgg tgcttctgcg
     2221 ctcgaggcct tgaccgagga cccggccgat cttggcattt tcgacatcaa gatgccgcgt
     2281 atggacggca tggagctgct tcgccgcgtg cgccagcagt cggacatgcc ggtgatcttc
     2341 ctcacctcca aggacgagga gatcgacgag ttgttcggcc tgaaaatggg cgcggacgac
     2401 tacatctcca agccgttctc gcagcggctg gtcgttgagc gggtcaaggc ggtgctgcgg
     2461 cggttccaac cgaaggacgg cagcaattca caatcggaaa ctgcgcgcgt catcgagcgc
     2521 ggccagctgc gtctcgatcc cgagcgccac acgtgccact gggacggcaa gcccgtgacg
     2581 ctgaccgtca ccgagttcct cattcttcaa gcccttgcgg cgcggcccgg cgtggtcaag
     2641 acgcgcgacg cgctcatgga tgcggcctat gacgatcagg tttacgtcga cgaccgcacc
     2701 atcgacagcc acatcaagag attgcgtaag aaattcaagc aggtcgacga gaattttgac
     2761 gtgatcgaga cgctttatgg tgtagggtat cgcttcaagg aggtttgagc cccggggggt
     2821 tcatggcgat tgaactggag cgctccggac cgatcgccgg tccgtggcgg gctgcgagag
     2881 agagcttcct gcgcgtgcat cgcgctgcga ggcccatgag ggagttcctt gcggccgtgg
     2941 gcaatcgcct gctgcgcagc cgagcctttc ggctggtcac gggcagcctg ctgcggcgca
     3001 tcttcgtctc caacctgctc ggcctgctga tcctcgtcgg cgggatactt tacctcagca
     3061 agcacaacgt ctggctgatc gacgccaagc gtgagagctt gcgcagccag agcgagatca
     3121 tcgcggccgc catcgccgcc aatgcgaccg tcgacaccga gcggatcgtg ctcgatcccg
     3181 acaagctgcc ggaggccgaa gacgctctca tcccgttccg ggacgacggc ttcgccgcct
     3241 tgcagctctc gatccggccc gaacgggtca caccgatcct gcgccgcctg gtccagccga
     3301 cgaacaaccg cgcgcgcatc tacagtcgcg acggcacact gatcgccgac tcggcgcaac
     3361 tcctgtcccg tggcctcatc gccaagccgc cgccgccaaa agcccggccc gacaccaaga
     3421 atttctggac ccgcctgacg aaatggctgc tgaatgccga tcttccggtc tacaaggaaa
     3481 tcggcaacgc caacggcaac ttctatcccg aagtccgtat ggccatgacc ggcaccacga
     3541 cgccgatgct gctgctcaac gaggacggcg agcagatcgt gtcggtcgcc gctccgatcc
     3601 agcgcgcccg ctcggtgcag ggcgtccttc tgctgtccag caagccgggc gagatcgatc
     3661 aggcgctggc ccaggaacgc tggacgatct tgaagatcgc gctgatggcg ctcgccgcga
     3721 ctctggccgc ctcgattctg ctggcgcgta ccgtcgccgg gccgatgcgg cgactgtcgg
     3781 aagcggccga gcacgtcagc cgcaacatct cggcgcggca cgatctgccg agctacggcg
     3841 atcgcagcga cgaggtcggc cagatggcga cggccttccg caacatgacg acggcgctca
     3901 tccggcgcgt ggaggcgagc gagaagttcg ccgccgatgt cgcccacgag ctgaagaacc
     3961 cgctgactgc ggcgcgctcg accgcggaag ctatcgctta cgcacgcgac gatgcggagc
     4021 gccgccagct catctcacag atgcaggatg agctgaagcg cctcaaccgg ctcatcacgg
     4081 acgtgtcgaa cgcctccagg ctcgatgccg agctcgccct ccagcagacc gagccggtcg
     4141 cgttgtgcaa gctcctcgac ggcatcgtaa acacgttctc ggacatcctc agcggcaccg
     4201 gcaagagcat cgcattcgag gtcgacccga ggtgctcgga ggattcgctc gtggtgattg
     4261 ggcacgaggg gcggctgtcg caggtcatca ccaaccttgt cgacaacgcc atttccttct
     4321 cgccggaggg cggcacggtg ctggtgcgcg ccgggcgttt cggcaacttg atcgagattc
     4381 gggtggagga cgatggtcca ggcatcccgc ccgagaacct ggagaagatc ttccggcgct
     4441 tctacaccta ccggcccacg gcagagagca gccgcggcaa caactcaggc ctcgggctct
     4501 cgatctcacg cgagatcgtg accgcgcacg gaggtgagat ctgggcagag aaccggatgg
     4561 ccgatggcgc cggcccggat gatcggccgc tcggtgcgcg tttcgtcatc cagttgcccg
     4621 cgaaaccggt ttctggaccg ctggcgagga gcttcggccg tggccaccgc cggggctaga
     4681 ctgatccatg ggaccgcgat tgcgctcgcg ggacgcgctg ctttgatccg tggcgcaccg
     4741 ggctccggaa aatccgacct tgctctccgt tgcctggcac aggcctcctc cgatctgatc
     4801 tcaggtcagg cactgctggt cgcggacgac caggtgtatg ctgaagtgct cgacggcaag
     4861 gttcaacttg gttgcccgga gcccatcagg ggtttgatcg aggtgcgcgg catcggcatc
     4921 gtcgccgtgc cggcgcaggg gagcgccgtg ctcgagttgc ttgtggatct cgtcccccga
     4981 cacgaggtgg aacggttgcc tgatcccgag gcggccgcgg agctgttcgg catccgcatc
     5041 cggcggctgg gcctatatcc cttcgacgca tccgcaccgc tcaagttgct gcttgcgctg
     5101 cagggaaaag gggccattac aaatggctcg cagtagccaa gatgggcaaa agcgcttgct
     5161 tcgcatcatg acacttgcca aacatgtcgc ggctcgaaac cgtcaattgc ctgctagtga
     5221 catgcgagag ccggtcggcc gccaagccgc caccggcctg cacggaactg ctggaaagcg
     5281 atgattggac ttttgatcgt cacccatggc ggactggccg gtgagttccg ggccgcactg
     5341 gagcatgttg tcgggccgca agagcaaatc gaaaccatct cgatcgggcc ggacgatgac
     5401 atggaggctc gcaggcagga gatccttact gcggcccgtc gcctcgacac cggagaggga
     5461 gtcattctcc tgaccgacat gttcggcggc acaccttcga acttgtcgat atcgatcctg
     5521 gacgaagcca acgtggaggt gattgccggg gtcaatctcc ccatgctcgt ccgcctcgcg
     5581 cgggtgcgca gcgatacgcc gatcgaaaag gcggtggatc tggcgaagga agccggtcgc
     5641 aagtacatta gcgtcgccag acaggtcctt tcaggtgaac cctgaggcag aggaacgcac
     5701 gtgctggaca gacgcaagcc ttcggctgtc gtgaccattc gcaatcgcaa gggattgcat
     5761 gcgcgcgctt ccgcgaagtt cgtgaagtgc gccgagtcgt tcaatgcaac cgtctccgtg
     5821 acccgcgatg gcaacaccgt cgggggcacc tccatcatgg ggctcatgat gctcgccgcg
     5881 ggtcccggct cgactattca tctcgaagca gaaggacctg aggggccgga agccctcgag
     5941 gcgctggttt cactcatcga ggacggcttc ggcgaggatt gcgtggacga atgctagcgc
     6001 gcgcgtgacc tcatcagatc cgcgcgcacg ggattgacgg tcaccatggg cggtacccct
     6061 cgcatttcag ccgagtcaaa gacataacaa tgtctttatg tgcggttgcg tgccaggcga
     6121 taggccagta tagacggccg cgggcgagcg cgtgcgggag accttccccg ccatccggcg
     6181 ttcgccattt caccgccgcc cgaggcggga caaccgatcc gatgacgagg agcccagaat
     6241 gccgatcgcc cgcgaatacg ccgtcaagga catcggtctc gccgagtggg gacgcaagga
     6301 gatatcgatc gccgagaccg agatgccggg cctcatggcg acgcgcgcgg agttcggccc
     6361 gggccagccg ctcaaaggcg cccgcatcgc cggctcgctg cacatgacga tccagacggc
     6421 cgtgctcatc gagacgctgg ttgcgctcgg tgcggaggtt cgctgggcgt cctgcaacat
     6481 ctactcgacg caggaccacg ccgccgccgc catcgctgcg gccggggtgc ccgtgttcgc
     6541 gatcaaaggc gagtcgctcg aagactactg gaactacacc cgcaagatca tggagtgggg
     6601 cgacggcggc acgcccaaca tgatcctcga cgacggcggc gacgccaccc tcctgatcca
     6661 cttgggcgtg cgggccgaaa acggcgatac ggccttcctc gacaggcccg gctccgagga
     6721 ggaggagatc ctgttcgcgc tcatcaagcg gacgctgcag gagaagaagg gctggttcaa
     6781 cgaggtcgcc cgcaacatcc gcggcgtctc cgaggagacg acgaccggcg tgcaccgtct
     6841 ctacgagatg cagaagaagg gcacgctgct gttcccggcc atcaacgtca acgactcggt
     6901 gaccaagtcg aagttcgaca acctctacgg ctgccgcgaa agcctcgtcg acggcatccg
     6961 ccgcggcacg gatgtgatga tggccggcaa ggtggccatg gtctgcggct tcggcgatgt
     7021 agggaagggc tcggccgcca gcctcaggaa cgcgggctgc cgcgtcatgg tctccgaggt
     7081 cgacccgatc tgcgccctgc aggcggccat ggaaggctac gaggtcgtca ccatggaaga
     7141 tgcggcgccc cgcgccgaca tcttcgtgac ggccaccggc aacagggacg tgatcacgat
     7201 cgaccacatg cgcgccatga aggaccgcgc catcgtctgc aacatcggcc acttcgacaa
     7261 cgagatccag gtcgcggcgc tgcgcaactt caagtggaac aacatcaagc cgcaggtcga
     7321 tgagatcgag ttcccggatg gcaagcgcat catcctgctg tcggaaggtc ggctggtgaa
     7381 cctcggcaac gccactggcc acccctcgtt cgtcatgtct gcgtcgttca ccaaccagac
     7441 gctggcgcag atcgagctct tcacgaatcc gggcaagtac gagcgcaagg tttacacgtt
     7501 gcccaagcac ctcgacgaga aggtcgcgat gctgcatctg gccaaggtcg gggcaaagct
     7561 gacgaagctg acgccggcac aggcggagta tatcggcgtc tcaccggagg gcccgttcaa
     7621 gtcggaccac taccgctact gacggctgac ggctggcgcg gcggtcttca aagccttgtc
     7681 gtgatgtgat cacgtgatac tctgcgcgcc gaggggcacc gcggcaccgc gagctccctc
     7741 gaaggaaaag gcaattcacg ggaggtcacg atatgacgct agggcggcgg ttcacactgg
     7801 gattagcggc catcgccacc gccgcattca cagcaacggc ggtggcatat ccgacgaagt
     7861 cgatcgacta catcatcgcg ttcggaccgg gtggggaatc cgacatcacg gcgcgcctgc
     7921 agcagccgtt tttcaagcaa aagttcggcc aggatcttgt ggtctcctac aagccgggcg
     7981 gcggtggcgc cgtggcctgg gcggagctga actctcatcc cggcgacggc cacaccattg
     8041 tgggtgtgaa cctgccgcac atcgtgctgc agcccgcaca gaaggacgtc ggctacaaga
     8101 ccgaggatct gaccgtcgtc tactggttcc acttcacgcc ggacgccatc gtggtgaggg
     8161 cagacagtcc gtacaagacg ctccaggatc tcatcgacga cgccaagaag aaccctggga
     8221 aggtgacgtt ctctggcagc ggcaagggga ctgcgaacca cttggcgcaa gtccgcttcg
     8281 acaagatggc cggcatcaag accacttatg tggcttttgc gggaaccggt ccttccaata
     8341 ccgccctgct cggcggccag acctcggctg cctggggata cacgaccagc gccgcagcta
     8401 tcggcgatca ggcgcgcata cttgccgtgg ccatggagga gcgtcatccg aagtatccgg
     8461 acgtgcccac gttcaaggag ctgggcttcg atctggtctc gggcgcctac cgcggcattg
     8521 cggtcccgaa atccacgccg gaggacatcc gcaagaagct ctccgacatg attggcgaaa
     8581 tcaacgccga tcccgagttc aagaagaaga tgctggatgg cggctacgcc atgctcgacg
     8641 tcccctacga aaagaacgcc gagttcatgg cgacccagtc caaagccttc atcgaggccg
     8701 ccaaggaagc cgggattctg aagtaggccg tctgacggta tcgctatgcg ccggcccgtt
     8761 cagggccggc gcattttgcc tcctgcttcg agaaccgaga gccatcgaga actcctgaat
     8821 gtcaggcctt gagacgctgc tcgcggcgct aactccgatc aatctgctac tggcactcgc
     8881 tggtgtgatc gccgggacgg cgattggcgc tattccaggg ctaacggcga ccatggcggt
     8941 cgccgtactg gttcctttca cattcggtat gccgcctgcg ccagccctaa ttttgatggg
     9001 cgccatctac accggtgcga tttatggcgg cgcctactcc gcaattctct tgaatactcc
     9061 cggcacaccc tccgccattg ccacgacgct cgacggctat cccatggccc ggcgcggaga
     9121 tggcgacctc gccatgacac ttgcctgcct cgcaagcgtc ttcggcggac taatcggcgc
     9181 attggcgctg ctgagcctag cacctcccct gtcgcgcgtg gccctcgcct tcggcccggt
     9241 cgagtacttc tggttggcca tcttcggtct ctctctgatc tcggcgctct cggaggggag
     9301 cctgctgaaa gggcttgcgg gcggcgcctt tggtctcctc atctcgtgcg ttggcgtggc
     9361 ggaagtgtcg gccgacgtgc gactcacatt cggctctcag acgctgatcg gaggcataaa
     9421 cgtcattgcg gcactgatcg gcctcttttg tatcccggtg ttgatcgacc tcgtcgcgac
     9481 gcccggccgc cacctcaacg tggtcgccga accgcgtggc ttccggttgg cggaggccgt
     9541 gcgggtctta atccgaaaga aattcaatct catacgttcc tcggtcatcg gcacgatcgt
     9601 cggcatcctg ccaggcgctg gtggctcggt ggcaggaatc gtcgcctatt ccgaggcccg
     9661 gcgcgcggcg agaccggagg aggaatacgg caaaggtgag ccaagcgggg tgatcgccag
     9721 cgaatccgcc aacaatgcca ccgtcggcgg cggcttcatc ccgacacttg tcctcggcat
     9781 cccaggtaca ccgccggatg cggtcatcct gggcgccctg cttgtgcaag gcttacgcac
     9841 ggggcccagc ctgttcaccg tccacgccga tatcatctac acgttcatct ggggactact
     9901 gatcgccacg atcctgatgt tgccgatcgg cctcctgctc gggcgctacg catacaagag
     9961 catcatcaca ttgccgaagg ccttgctggt cccctcggtg gcattcatga ccatgatcgg
    10021 cacctacgcg atccgcaaca atatctctga cgtcgtgatc atggtcgtcc taggcgtgat
    10081 cggctggatc ctcgggcggt tcgggtttgc ggcggctccg atcgtgctgg ggctcatcct
    10141 cggccctatc gccgagcagg gtttcgtcca ggcctggacg atcggcagcg cccaggagga
    10201 cctcgtcggc atgttcttcg gccgcccgat ctcgatcgcg attatctttt tcacgatctt
    10261 ctcgctggcc agcccgatcc tgatgcgccg ctggaaagtc aagccggagg ccgttcatgt
    10321 cgaatgaaag gatgccagcg cagtcggccg acgtgggcac tgcgatcatc gcatccctgc
    10381 tgatcatctt cggcgccgca gcgatctggg acaccttcag ctatgccgac ttcgattcgg
    10441 cggtgtttcc ccggatggtg gcaggcgtgc tgatcctgat tgcggtcggc gttctggtac
    10501 ggtgggtcgc gcagcggatt ggcgccatcg cgatgacgcc aacggaagtt gaggagagcg
    10561 gcggatcctg gccgcgccgc attttgctag tcgtggtaat gttggcggcc gcgctggcga
    10621 tgccctggct cggtttcctg gtggcggcca tcatcagctt cgcagccctg ctgctgatcg
    10681 ccatgcacga cccatggact ccgtcgcgaa tcgtcgtcta tcccctcgtc gggctggcc
//
LOCUS       DASVDU010000284        10472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_876401, whole genome shotgun sequence.
ACCESSION   DASVDU010000284 DASVDU010000000
VERSION     DASVDU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876401"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF226_18975"
     CDS             complement(<1..288)
                     /locus_tag="VF226_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076127.1"
                     /translation="MLGRVTVFAVVMAVLVAVPPFLGIGSQNALTNMLIASLFAMAFN
                     LLIGQAGLLSFGHAAFFGVGAFAALHLMLAIEDRVVSFPTPLLPLAGAAAGF"
     gene            complement(299..1240)
                     /locus_tag="VF226_18980"
     CDS             complement(299..1240)
                     /locus_tag="VF226_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076128.1"
                     /translation="MEFLIISTLNGVIYGLLLFMVSSGLTLIFGMMGVLNFAHASFYM
                     LGAYIAYTVTTTVNFWAGLIIAPILVGLLGVFIERHFLRRVHVHGHAHELLLTFGLAF
                     IFEELIKLFYGDFPVNYAMPDNLRFSAFTIYGTDYPFYRLFIGLVAIAMFVALYLLLT
                     RTRAGLVVRAAERLPTMAESLGHNVPFVFMGVFGTGAAFAGLAGAVAGAFFPTNPNMA
                     LELGVIVFVVVVVGGLGSLEGSLVASLLIGLFSSFAVGLDWSLAGLLSWVGLGDWARS
                     VGGLFTLTLSSVSGTVPFIVMLIVLLVRPAGLMGERN"
     gene            complement(1425..2663)
                     /locus_tag="VF226_18985"
     CDS             complement(1425..2663)
                     /locus_tag="VF226_18985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7076129.1"
                     /translation="MRGKSLSLAIGAAVALGLSAGAGHAEALKIGIIESLSGAQTSTG
                     RLFAAAAQFVVDDINKNGGFNGAPIEVIEYDNAGGTTGAADKFKQAVADGVHIVLQGA
                     SSAIAGQVTEDVRKHNIRNPGNEVMFINLGAEALELTGDKCHFYHFRFTTTAPMRVNA
                     LVNAMKKAGELGKRVYAINQNYSWGRDMEEATKAAAKVGGYEVVETVLHEVNKIQDFS
                     PFVAKIKAANPDTVITGNWSNDLLLLMKATGDAGLKVRFATAFLDQVGNIANAGKTAL
                     GHYVANPYNIEADNTGFPEAYKAATGHLPVYIEPQTVNAIKTLREALKTVDFKGGEIS
                     VTEIAKALEKASIETPIGKISVRAEDHQAVIPVVVSKVVENPKYPVDDTNLGFGVVEV
                     IPGPDAIYPVQSSCKMDRPS"
     gene            complement(2686..3501)
                     /locus_tag="VF226_18990"
     CDS             complement(2686..3501)
                     /locus_tag="VF226_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002968880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7076130.1"
                     /translation="MITGTTRILGIIADPVAHVRTPQIMNAYFAEHGVDAVIVPMQVS
                     GAGLTTVLDAVRQLRNFAGLIVTVPHKTAVVGLCDDALDEARLVGSANAIRRDDDGRL
                     VCDMFDGKGFLGGLLAEGIEPEGRKVLLLGAGGAASAIAVSLAGRGVSTLTIVNRTKD
                     KARALAARVAAAFPACQVAEGPPDPSGHDLVVNATSLGLRPDDPLPLDPDLLRPDMTV
                     AEVVMKPERTAFIEAAQARGCRVHLGRHMLDAQVRLLAGFLGVAAGAADLRQN"
     gene            3750..4490
                     /locus_tag="VF226_18995"
     CDS             3750..4490
                     /locus_tag="VF226_18995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076131.1"
                     /translation="MFDFAGKVLVLTGANGAIPRAVARTFFDLGAGLVLTDIETEGLA
                     AFARTLDPAGDRIVTLRQDAREPADAARLADACKERFGGADFLVTGAGLYRDQLVATM
                     SDAQWRESIGINLDGVFYTCRAIAPLLRDGGAIVNIASMAGHRGSYQHSHYAAAKGAV
                     LTFSRSLALELAPRIRVNAVSPGLIDTPLVQPLLAARGDELIAATPLKRLGTPDEVAR
                     AIAFLCSDWASFIDGETLHINGGLYIAS"
     gene            4500..5273
                     /locus_tag="VF226_19000"
     CDS             4500..5273
                     /locus_tag="VF226_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX7076132.1"
                     /translation="MADRKSMDLEGSGSVPLVRPSGLPDEIAKLLRNEILKGTLKPGD
                     KLPSEQQLCRMFGVSRPVVREAISRLKYDGVLESFQGRGVFVRGDGSGPSFRIDEPNL
                     ADQSELAHIIELLVAIEGAATGLAAARRSQQELEAIKRALNAMARAIDEGLNGVDEDV
                     AFHRTIVEATRNPFFIALVAFLESRVRNLIRAARTNTARYEGLAYKVQEEHEAIYEAI
                     AAGDPDAAKAAAERHLRNAAARLKLYQEERSSKARVDKA"
     gene            5427..7727
                     /locus_tag="VF226_19005"
     CDS             5427..7727
                     /locus_tag="VF226_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEX7076133.1"
                     /translation="MDKINQRIAAELGVGVAQVAAAVALLGEGATVPFIARYRKEKTG
                     GLDDTQLRKLEERLGYLRELEDRRATVLKSIEEQGKLTPELARSIAAAETKVELEDLY
                     APYKPKRRTRAQIAREAGLEPLADLLLKTPTLVPESEAAKFVDTEKGVADTKAALDGA
                     RHILIERIGEDAKLVGALREWLWTEGVLTSKVLKGKDTEGAKFADYFDFSQPIKDVPS
                     HRALAILRGRKEGILDIDLDVPHEADKPHPAELKIRAAFSIADRGRPADRWLADTVRL
                     AWKAKLAISLSVDLLVRLKERADTEAISVFSKNLKDLLLAAPAGPRVTMGLDPGYRTG
                     VKVAVVDQTGKLVDTATIYPHEPQRDWQGALATLAALCMKHKVDLVSIGNGTASRETD
                     RLVADLMAKMPQLKLTKVVVSEAGASVYSASELAAKEFPDLDVSLRGAVSIARRLQDP
                     LAELVKIEPKAIGVGQYQHDVDQTGLARSLDAVVEDCVNSVGVDVNTASVPLLARVSG
                     LNRTLAANIVAFRDENGAFKSRSALKKVPRLGPKVFEQAAGFLRIMNGKNPLDASAVH
                     PEAYPVVERIAERTGRPVKSLIGDRALLKTLKPADFADETFGIPTVTDILAELEKPGR
                     DPRPEFKTASFREGVEKISDLKPGMQLEGVVTNVAAFGAFVDIGVHQDGLVHISELSD
                     RFVKDPREVVKAGDVVKVRVKDVDVERKRIALTMKSGAIDRSTRDNARSSSVPHRARN
                     GAPQRSPDAPGESALAAALRRAQERK"
     gene            7897..8031
                     /locus_tag="VF226_19010"
     CDS             7897..8031
                     /locus_tag="VF226_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076134.1"
                     /translation="MRAYEKPDLIKREPLTQITAHDFFSPPVVIEKEVDGPPITATAE
                     "
     gene            complement(8073..9236)
                     /locus_tag="VF226_19015"
     CDS             complement(8073..9236)
                     /locus_tag="VF226_19015"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7076135.1"
                     /translation="MPVLLVIALGCFSGALSMRAIDPVVPEIARDLDTTAATVAMLAS
                     AFAFPYALGQPLLGPLSDALGKARIIKVCLAVLAATLFASAMATSIEALFVARVLSGI
                     AAGGIIPVGMAMVGDRFSIAERQVAISRIILAAVAGQLTSSIGAGFLASFFGWRFVLA
                     FTGAVAAAVLIASIISLAPRPKAERQQFTMAGLKAGYKAIFSNPRAAICYGAVAIEGI
                     VVYGMSPYVATLFEAQGIGGMREAGVVIAGIGIGGVVYAALVRVLLKRLGGPFNVMRG
                     GAIAAALGFSTIALQPAWPVQLIGFMLVGLGFFMIHNALQVQATELAPEARGSAVALH
                     AFFFFLGQAAGPVCYGLGFASIGVWATIMVSAATMLILGFLSAMGLERLAARP"
     gene            9553..>10472
                     /locus_tag="VF226_19020"
     CDS             9553..>10472
                     /locus_tag="VF226_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076136.1"
                     /translation="MGKTYDILILGASYGSLLATKLLLAGHNAKLVCLPNEVELFNAE
                     GAVVRMPVKGRDGLVEVRSRDLPGKLSADGPDSINPGDYDLVALAMQEPQFRAQGVRD
                     LLDRIAKAGVPCMSIMNMPPLTYLKRIPGLDVEACRTAFTDPSVWDSLDPALMTLCSP
                     DPQAFRPPEEKVNVLQVRLPTNFKAARFERDEHTAILRQLESDIEAARFEVDGSKIEL
                     PVKLRVHDSVFVPLAKWAMLIAGNYRCVLEDSVRPIKEAVHSNLDESRAVYNWVVELC
                     VKLGADEKDMVPFEKYANAALSLGSPSSAAR"
BASE COUNT         2024 a         3382 c         3367 g         1699 t
ORIGIN      
        1 gaaaccggcg gcggcgccgg cgagcggcag cagcggcgtc gggaacgaca ccaccctgtc
       61 ctcgatggcg agcatcagat gcagtgccgc aaacgcaccg acgccgaaga aggcggcatg
      121 tccgaaggac agaagccctg cctggccgat cagcaagttg aatgccatcg cgaacagcga
      181 cgcgatgagc atgttggtca aggcattttg cgagccgatg ccgaggaatg gcggcaccgc
      241 taccagcacg gccatgacga cggcgaagac ggtaacccgg cccagcacgg cgcccccctc
      301 agttccgctc gcccatgagc cccgccggtc gtaccagcag gacgatgagc atcacgatga
      361 agggaacggt cccggagacc gaggacaggg tcagcgtgaa gagcccgccg actgagcggg
      421 cccaatcgcc gagcccgacc cacgacagca gccctgcgag cgaccagtcg agccccacgg
      481 caaacgagct gaaaagcccg atcagcaggg atgcaaccag cgatccttcc agcgagccga
      541 ggccgccgac cacgacgacc acgaacacga tgacgccgag ctcgagcgcc atgttcggat
      601 tggtggggaa gaatgcgccc gccaccgcgc cggcgagccc agcgaaggcg gcacctgtcc
      661 cgaaaacccc catgaagacg aacggcacgt tgtgcccgag cgactcggcc atcgtcggca
      721 gccgctcggc ggcgcgcacc accagcccgg cgcgggtgcg cgtcagcagg agatagagcg
      781 cgacgaacat cgcgatggcg acgaggccga tgaacagccg gtagaagggg tagtcggtgc
      841 cgtagatggt gaacgcggag aaccgcagat tgtccggcat cgcgtaattc acgggaaaat
      901 cgccgtagaa gagcttgatc agctcctcga agatgaaggc gagcccgaag gtcaggagca
      961 gctcatgggc gtggccgtgg acatggacgc ggcgcaggaa atgacgctcg atgaacacgc
     1021 cgagcaaccc caccaggatc ggcgcgatga tgagcccggc ccagaaattg accgtggtcg
     1081 tgaccgtgta ggcgatgtag gcgccgagca tgtaaaagct cgcgtgcgca aaattcagca
     1141 cgcccatcat gccgaagatg agcgtcaggc ccgaggacac catgaacagc agcagtccgt
     1201 agatgacgcc gttcagagtc gagatgatca ggaattccat ggtgcgagcc ttgagagtcg
     1261 tcccggttca gatcggagcg gcagccacac gcacctcatg aacctctcct cggagaggtc
     1321 tgatttgcag caaccgagca cggatttcac ccagtcatat cagaagcata aacgggatgc
     1381 ggggctttcc tcccgcatcc cttgatctct cacaagcctt gttcttagga cgggcggtcc
     1441 atcttgcagg acgactggac cggatagatc gcgtcgggac ccgggatgac ctcgacgacg
     1501 ccaaatccga gattggtgtc gtcgaccgga tacttcgggt tctcgacgac cttcgatacc
     1561 acgaccggga tgaccgcctg atggtcctcc gcgcgaacgc tgatcttgcc gatcggcgtc
     1621 tcgatcgagg ccttctcaag cgccttggcg atctcggtca cgctgatctc gccgcccttg
     1681 aaatcgacgg ttttcagcgc ctcgcgtagc gtcttgatgg cattgaccgt ctgcggctcg
     1741 atgtagacgg gcagatggcc ggtggcggct ttgtaggcct ccgggaaccc cgtgttgtcg
     1801 gcctcgatgt tgtagggatt agcaacatag tggccgagtg ccgtcttgcc ggcattggcg
     1861 atgttgccca cctggtccag gaatgccgtc gcaaaccgca ccttgaggcc ggcatcgccc
     1921 gtcgccttca tcagaagaag caggtcgttc gaccagttgc cggtgatcac cgtgtccggg
     1981 tttgccgcct tgatcttggc gacgaagggc gagaagtcct ggatcttgtt cacctcgtgc
     2041 agaacggtct cgaccacctc atagccaccg accttggcgg ccgccttggt ggcctcctcc
     2101 atgtcgcggc cccaggagta attctggttg atcgcgtaga cacgcttgcc gagctccccc
     2161 gccttcttca tggcgttgac gagtgcgttg acgcgcatcg gcgcggtggt ggtgaagcgg
     2221 aagtgataga agtggcactt gtcgccggtg agctccagcg cctctgcgcc gaggttgatg
     2281 aacatcacct cgttgcccgg attgcggatg ttgtgcttgc gcacatcctc cgtgacctgt
     2341 ccggcgatgg cagaggacgc gccctgcagc acgatgtgga caccgtcggc cacggcctgc
     2401 ttgaacttgt ccgctgcacc tgtcgtaccg ccggcgttgt cgtactcgat gacctcgatc
     2461 ggcgcgccgt tgaagccgcc gttcttgttg atgtcgtcca cgacgaactg tgcggcggcc
     2521 gcgaacagcc ggccggtcga tgtctgcgcg ccggacagcg actcgatgat gccgatcttg
     2581 agcgcctccg cgtgccccgc gccggcggac aacccgagcg ccactgccgc accgatggcg
     2641 aggctaagcg attttcctct catgcgattc tcccgtttct ccctgctagt tttgcctcaa
     2701 atcggcggct ccggccgcca cccccagaaa gcccgccaac aggcggacct gcgcatcgag
     2761 catgtggcgg ccgagatgca cccggcagcc cctggcttgt gcggcttcga tgaatgctgt
     2821 gcgctccggc ttcatgacga cctcggctac ggtcatgtcc ggccgcagca ggtcggggtc
     2881 gagcggcagc ggatcgtcgg gcctgagccc gagcgaggtg gcgttcacca cgagatcgtg
     2941 accgctcgga tcgggtggcc cttccgccac ctgacaggca gggaaggcag cggcgacgcg
     3001 agcggccaat gcccgggcct tgtccttcgt ccggttaact atggtgagcg tgctgacccc
     3061 ccggccggcg agcgagacgg cgatggccga ggcggcgccg cccgcaccga gcagcagcac
     3121 cttgcggccc tcgggctcga tgccttcagc gaggagcccg cccaggaagc ctttgccatc
     3181 gaacatgtcg cagacgaggc gcccatcgtc atcgcggcgg atggcattgg ccgaaccgac
     3241 gaggcgggcc tcgtcgagcg cgtcatcgca caagcccact accgccgtct tgtgcggcac
     3301 ggtgacgatg aggccagcga aattacgcag ttgtcgtacg gcgtcgagaa cggtggtgag
     3361 ccccgcaccg gagacctgca tcggtacgat gaccgcatca actccgtgct cggcgaaata
     3421 ggcgttcatg atctggggcg tacggacgtg ggccaccggg tccgcgatga tacccaggat
     3481 ccgcgtcgtg cccgtgatca ttggccctcc ctcacacatc tccgcggtat tgccgcggct
     3541 gtcctcgatc cggctgttca cacctcaagc acgatgttgc cacttatatg cttatctgac
     3601 aagttacttg cgtatagact caggcagcga tttggctaca gcgcttcgcc gccggaagtc
     3661 ggcgccgaac ggtagaccac cgccgcgccc gaggccatcg aggaaaggcg ctcttgccgc
     3721 aaatgccggc ggcggcaatg gaggatggga tgttcgattt cgcgggcaag gtgctggtcc
     3781 tgacgggcgc gaacggagcc attccgcgcg ccgtggcaag aaccttcttc gatctcggcg
     3841 ccggcctcgt tctcaccgac atcgaaactg agggacttgc cgcatttgcg agaacgctcg
     3901 acccagccgg cgaccgtatc gtcacgctgc gccaggatgc gcgcgagccg gcggatgcag
     3961 cgcggcttgc cgatgcgtgc aaggagcgct tcggcggagc ggacttcctg gtgacaggcg
     4021 ccgggctcta tcgcgatcag ctcgtcgcaa ccatgagcga tgcgcaatgg cgcgaatcga
     4081 tcggcatcaa tctcgacggc gtcttctaca cctgccgcgc gatcgcaccg ctgcttcgcg
     4141 acggtggggc catcgtcaac atcgcctcga tggcaggcca ccgcggcagt tatcagcata
     4201 gccattacgc agcggccaag ggcgcggttc tgaccttctc gcgcagcctc gcgctcgagc
     4261 tcgccccacg catccgtgtc aacgcggtat cgcccgggct catcgacacg ccactcgtcc
     4321 agccgctgct tgccgcgcga ggcgacgagc tgatcgccgc gacgccgctc aagcggctgg
     4381 gcaccccgga tgaggtcgcg cgcgccatcg cctttttgtg cagcgactgg gccagcttca
     4441 tcgacggcga gacgctgcac atcaatggtg gtctctacat cgcgagttga caaccggtca
     4501 tggcggacag gaaatcgatg gacctcgaag gatcaggttc cgtcccgctc gtccggcctt
     4561 cgggcctgcc ggacgaaatt gccaagctcc tgcgcaacga gatcctgaag ggcacgctga
     4621 agcccggcga caagctgccg tcggagcagc agctctgccg catgttcggc gtctcgcggc
     4681 cggtggtccg ggaggcgatc tccagactca agtacgacgg cgtgctcgag tcattccagg
     4741 ggcggggcgt gttcgtccgg ggagatggca gcggaccatc attccgcatc gacgagccga
     4801 acctcgccga tcagagcgag cttgcccaca tcatcgagct tctggtcgcg atcgaaggtg
     4861 cagccacggg ccttgccgca gcccgcagga gccagcagga gctcgaggcg atcaagcggg
     4921 cgctcaacgc catggccaga gccatcgatg aagggctgaa cggcgtcgat gaggacgtcg
     4981 ccttccatcg cacgatcgtc gaggcgaccc gaaatccctt cttcattgcc ctggtcgcct
     5041 ttctcgagag ccgcgtgcgc aacctcattc gcgctgcgcg caccaacacc gcccgctacg
     5101 aaggcctggc ctacaaggtg caggaggagc atgaggcaat ctacgaggcg atcgccgcag
     5161 gcgaccccga cgcggccaag gcggctgcgg aacgccatct gcgcaatgcg gccgcgcgat
     5221 tgaagctcta tcaggaggag cggagcagca aggctcgggt cgacaaggca taaggccgac
     5281 caagagttgg ctgctcggcc atccttgctc caaatgaacc cgatagatcg tcccgacccg
     5341 gcggcccggc catccacacg gcttcgcagg ggaagagaat cgcgccattt accggcgctc
     5401 tgcgtgagcg acatcgcgag gacttcatgg acaaaatcaa ccagcgcatc gctgcagagc
     5461 tcggcgtcgg cgtggcgcag gtcgcggctg cggtggcgct tctcggcgag ggcgcgacgg
     5521 tgccgttcat cgcccgctac cgtaaggaga agaccggcgg cctcgacgac acccagctga
     5581 ggaagctgga ggaacgcctg ggctacttgc gcgagctgga ggatcgccgc gcaaccgtgc
     5641 tcaagagcat cgaagaacag ggcaagctga cccccgagct cgcccgctcg atcgccgccg
     5701 ccgagaccaa ggtcgagctg gaggaccttt acgcgccgta caagcccaag cggcggacgc
     5761 gggcgcagat cgcgcgggaa gctgggctgg agcctttagc cgaccttctc ctcaagaccc
     5821 caacactggt gccggagagc gaagccgcaa agttcgtcga tactgaaaaa ggagtggcgg
     5881 acaccaaagc ggcgctcgac ggagcccgcc acatcctcat cgagcggatc ggcgaggatg
     5941 caaagctcgt cggcgcactg cgcgaatggc tgtggaccga aggcgtgctc acctcgaagg
     6001 tgctcaaggg caaggatacg gaaggggcca agttcgccga ctatttcgac ttcagccaac
     6061 ccatcaagga cgtgccctcc caccgggcgc tcgccattct ccgcggccgc aaggaaggca
     6121 ttctcgacat cgatctcgac gtcccgcacg aggccgacaa gccgcatcca gccgagctca
     6181 agatccgggc cgccttcagc attgccgatc gcggccggcc ggcggaccgg tggctcgccg
     6241 atacggtccg cctggcgtgg aaggcgaagc tcgccatttc gctcagcgtc gacttgctgg
     6301 tgcgcctcaa agagcgggcc gacaccgaag cgatctcggt cttctccaag aacctcaagg
     6361 atctgctgct tgcggcgccc gccggcccgc gcgtcaccat ggggctcgac cctggctatc
     6421 gcaccggagt caaggtcgcg gtggtggacc agacgggcaa gctcgtcgac acggcgacca
     6481 tctacccgca cgagccgcaa cgtgactggc agggcgcgct tgccacgctg gcggcgctct
     6541 gcatgaagca taaggtcgat ctcgtcagca tcggcaatgg tacggcaagc cgcgagacgg
     6601 acaggctcgt ggccgatctg atggccaaga tgccccagct gaagctcact aaagtggtgg
     6661 tttcggaggc gggggcttcg gtctactcgg cttccgagct ggccgccaag gaattccccg
     6721 acctggatgt gagtctgcgc ggcgcggtct cgattgcccg tcgtctgcag gatcctctcg
     6781 ccgagctggt caagatcgag cccaaggcca tcggcgttgg tcagtatcag cacgacgtag
     6841 atcagacggg gcttgcccgc tctcttgatg ccgtggtcga ggattgcgtc aactccgtcg
     6901 gcgtcgacgt caacacggca tccgtcccct tgttggcgcg cgtgtccggc ctcaaccgga
     6961 cgctggctgc caatatcgtt gccttccgcg atgaaaacgg tgccttcaaa tcgcgaagcg
     7021 ccttgaaaaa ggtgccgcgc ctggggccga aggttttcga gcaggcggcg gggtttctgc
     7081 ggatcatgaa cggcaagaac ccgctcgatg cttccgcagt ccatcccgaa gcctatcccg
     7141 tggtcgagag gatcgccgag aggactgggc gtcctgtgaa atccctcatc ggtgaccggg
     7201 cgctgctcaa gacgctgaag cccgctgact tcgcagatga gacgttcggc attcctacgg
     7261 tgacagacat cctggccgag ctggagaaac caggacgcga cccgcgcccc gaattcaaga
     7321 cagcctcctt ccgggagggc gtcgagaaaa tctcggacct gaagcccggg atgcagctcg
     7381 aaggggtcgt caccaacgtc gctgcgttcg gcgccttcgt cgatatcggc gtgcatcagg
     7441 acgggctggt gcacatctcc gagctgtcgg atcgcttcgt caaggacccg cgcgaggtgg
     7501 tgaaggccgg cgatgtcgta aaggtgcggg tcaaggatgt ggatgtcgag cgcaagcgca
     7561 tcgcgctcac catgaaatcg ggcgcgatcg atcgatccac gcgcgacaac gctcgctcgt
     7621 cgagcgtgcc acaccgcgcg cgcaacggcg ccccgcaacg atcccccgac gctccaggtg
     7681 aaagcgcgct tgccgccgcg ctgcgccgtg cgcaggagcg gaaatagaac cgatggggcg
     7741 atggcggccc agctcgaacc catcggaagg aggtcggggc tgatacggtg gcgcttcggc
     7801 ttcatgcatc cacaggttgc gaaaacttga atgttctccc gaacattgtt caactaatct
     7861 ggcctccctc gatacgagag aggaggtcac cgcccgatga gagcgtacga gaagcctgat
     7921 ctgatcaagc gcgagccgct cacccagatc acggcccatg actttttcag tccgccagtc
     7981 gtcatcgaga aagaggtgga cgggcccccc attacggcca cagccgaatg accacgctct
     8041 ccggtgggtc cgcctgccgg agaggcagct gatcacggcc gcgcggcgag ccgctcgagc
     8101 cccatcgcag agaggaagcc gagtatcagc atcgtcgccg cgctgaccat gatggtggcc
     8161 caaactccga tcgaggcaaa ccccaggcca tagcagacgg gccccgccgc ctgtcccaga
     8221 aagaagaaga atgcgtgcag cgcgacggcg gagccgcggg cttccggcgc cagctccgtc
     8281 gcctgcacct gcagcgcgtt gtggatcatg aagaatccga gcccgaccag catgaagcct
     8341 atgagttgaa cgggccaggc cggctgcagc gcaatcgtgc tgaacccgag cgcagcagct
     8401 attgcaccgc ctcgcatgac attgaacggt cctccgaggc gcttcaaaag cacgcggacg
     8461 agcgccgcat agacaacccc gccaatgccg atgccggcga tcaccacgcc cgcctcgcgc
     8521 atgccaccga tgccttgcgc ctcgaacagc gtggcgacgt acggggacat tccgtagacg
     8581 actatgcctt cgatcgcgac agctccgtag cagattgcgg cccgcgggtt cgagaagatc
     8641 gccttatagc cagccttcaa tcccgccatt gtgaactgct gccgttccgc cttcggccgc
     8701 ggagcgagac tgatgatgct cgcgatcaac accgccgcgg ccacggcgcc ggtgaacgcc
     8761 agaacgaatc gccagccgaa gaacgaggcg agaaatccag cgccgatgga gctcgtgagc
     8821 tggccggcga cggccgccag gatgatgcga gagattgcga cctgccgctc ggcgatggag
     8881 aaacgatccc cgaccattgc catcccgacg gggatgatgc cgccggccgc aataccggaa
     8941 agcacacgcg ctacgaagag cgcctcgatg gaggtcgcca tggccgaggc gaaaagggtg
     9001 gccgccagaa cggcgaggca gaccttgatg atccgtgcct tgcccagcgc gtctgacaac
     9061 ggcccgagca aaggctggcc gagggcatag ggaaacgcga acgccgacgc cagcatcgca
     9121 acggttgcag ccgtcgtgtc aagatcgcgg gcaatctccg gcaccacagg gtcgattgcg
     9181 cgcatcgaca gcgctccgga aaagcagccg agcgcgatga cgagaagaac aggcattgcc
     9241 gcgtcggagc ccgcaaagaa cagctgaaga tgtgcccggc ggacgacgaa gtctgcagga
     9301 aggcgcaacc gtaaggcgca acctccccac cgtcgagagg ggaacctgcg ccgatgccga
     9361 tgagctggta tgcgcttgcc gagcgatcgt aggctcacgg gttggcgctc gtgatgccgg
     9421 ccgacgccga acaggcccaa aaatggactg gtgattgcct cacgagccct gactctttag
     9481 catgttcggc ggaccgggcg ggctgccgca ccggagtcat cctcgcatag ggagcggaaa
     9541 gagcgccgtc acatgggcaa gacatacgat attctgatcc tcggagcctc ctacgggtcg
     9601 ttgctggcaa cgaagctgct gctcgctggc cataacgcga agctggtctg ccttcccaac
     9661 gaggtcgagc tgttcaatgc cgagggcgcc gtcgtgcgca tgcccgtcaa ggggcgcgac
     9721 ggcttggtcg aggtcaggtc gcgcgacctt ccgggcaagc tctctgccga cgggcccgac
     9781 tcgatcaatc cgggcgatta cgatctggtg gcgctcgcga tgcaggagcc gcagtttcga
     9841 gcccaaggcg tgcgcgacct gctcgatcgg atcgcgaagg ccggtgtgcc ctgcatgtcc
     9901 atcatgaaca tgccgccgct cacctatctg aagcgcatcc cagggctcga cgtggaagcc
     9961 tgccgtaccg cgttcaccga tccgtcggtc tgggacagcc tcgaccctgc gctcatgacc
    10021 ctgtgcagcc cggacccgca ggccttccgt ccgccggagg agaaggtcaa cgtcctgcag
    10081 gtgcgcctgc cgaccaactt caaggccgca cgctttgaaa gggatgagca tacggccatt
    10141 ctgcgccagc tcgaatccga catcgaagcg gcgcgattcg aggtggacgg cagcaagatc
    10201 gagctgccgg taaagctcag ggtgcacgac tccgttttcg tgccgctggc gaagtgggcc
    10261 atgctgatcg ccggcaacta tcgttgtgtg ctggaggatt ccgtgcggcc gatcaaggag
    10321 gccgttcact ccaacctcga tgagtcgcgg gcggtttata actgggtcgt ggagctgtgc
    10381 gtcaagctcg gcgcggacga gaaggacatg gtgcctttcg agaaatacgc caacgccgcc
    10441 ctgtccctcg ggagtccttc gtccgcggcc cg
//
LOCUS       DASVDU010000285        16407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_278649, whole genome shotgun sequence.
ACCESSION   DASVDU010000285 DASVDU010000000
VERSION     DASVDU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16407
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278649"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VF226_19025"
     CDS             complement(<1..1005)
                     /locus_tag="VF226_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7076137.1"
                     /translation="MSASPAANPLLVPWSTPFGAPPFAEIAPEHFRPAFERALAEHRS
                     EIDRIAGDPAPPTFDNTIAALENAGRLLDRVSAVFWNLASADTNDALQAIEREISPKL
                     AQHWNAITSNAALFARIDAVMQGAESADLNPEQRRVLERTHLAFVRSGARLGPEEKKR
                     LGEIMERLAALGTQFSQNVLADEKSYELPLTEEDLAGLPDFLIDAARRTAEERGSAAK
                     FVITLSRSLIEPFLTFSPRRDLREKAFTAWTRRGENGGATDNRGIIAEMLKLRRERAR
                     LLGYETFADYKLDNTMAKTAANVRALLNEVWPAAKRRALQEREKLQAIAQREGANITI
                     E"
     gene            complement(1085..2056)
                     /locus_tag="VF226_19030"
     CDS             complement(1085..2056)
                     /locus_tag="VF226_19030"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7076138.1"
                     /translation="MDTQLRSFHTVATHGGFTAAANVLNIGQPTISSQVKALEERYRV
                     ELFHRRGRRVELTETGRRLLELTQRIASLESEAVDLLNAVGGLRSGHLRVSAVGPYHV
                     TEMLSAFRDRYPGVEFSVRISNSREALRQLMDYKADVAVLAHTEDDPRIHAVPYSRHP
                     VIAFVNSDHPWAGRTSVSIHEFAEQHVILREVGSTTRLAFEAGLASAGARMGSFTEIG
                     SREAIWLAVERGMGVGIVSEFELIPHPRLHPLPFRDAEIYTYAHVVCLAERRQGPLVR
                     AFFGVVESLLAARRNVTETGRALIRQPPGKSGRREKGRKTSGLGARV"
     gene            2155..3249
                     /gene="psrA"
                     /locus_tag="VF226_19035"
     CDS             2155..3249
                     /gene="psrA"
                     /locus_tag="VF226_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase PsrA"
                     /protein_id="HEX7076139.1"
                     /translation="MWTYHNPVRILFGEGTFDKLGSLIAGRRYVLVTYGEPLFRELAA
                     RLARSAGAPALVVDDVAPNPDRADLSRQCQRWNELAEAPQLVVAVGGGSVIDTAKVIA
                     AARGNFGPVWEVLITGRGAERLEPLPIIAVPTTAGTGSEVTSWATVWDGEGGRKYSLA
                     AHALHPVHAVLDPELTLSCPKGLTISTGLDALSHALESIWNRNANPVSAVHAVAAARE
                     ILEVLPALSLRLEDRALRARMLRAAMFSGLAFSNTKTAIAHSMSYAVTLRHGTVHGIA
                     CSFTLPMVLRSVIGLNADCDAVLARIFGADLAAGAARLQGFLEDLGVSTRPADHGVGG
                     EEWRSIVEDALGGERGLNFLGSKEGLLAQI"
     gene            3356..4255
                     /locus_tag="VF226_19040"
     CDS             3356..4255
                     /locus_tag="VF226_19040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7076140.1"
                     /translation="MKLRTLFAAAISALLAMTGAASAQEKCENPEALRFSIIPTEETM
                     QELALYKPVIDILSRNTGKKIEFYMPTSYASVIEAMLGNWVDIAVHGPNSYVIGNEKS
                     KNIEVFATYAKAKGIFQEEGPGYHAILIVRKDSKFKDIASLKGAVVALADPASTSGNL
                     MPRVEFAKEIGGGELESYFSKVVYSGSHDKSAVAVSEGKADAAFVATHRLDNVIERGL
                     VKAEDFAVLWKSRLVPQDPFTYRGTLCEDLKAKIRDTFLNLHNEPGAKKFLENLNSVK
                     FVAMKSEDYDLIRDLRAAQAKKK"
     gene            4341..5093
                     /gene="phnC"
                     /locus_tag="VF226_19045"
     CDS             4341..5093
                     /gene="phnC"
                     /locus_tag="VF226_19045"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HEX7076141.1"
                     /translation="MPILDVQDLSVRYSRNGAEILKGVSLQVDAGDFCAVIGPSGAGK
                     STLIRCINRLVEPRAGRILLYGEDVVTMSTRRLRETRRHIGMIFQEFNLVNRMSVMDN
                     VLSGRLGSAGTLRSLLKLFSREDIERALALLDRVGLSDHVDKRADQLSGGQRQRVGIA
                     RALMQDPKLLLVDEPTSSLDPKIAREVMTLIRDVAREFNVPALVNIHDVQLATEFCNK
                     IIGLQDGVKMFDGPTEQLDRKMLDTIYAMEVL"
     gene            5096..5932
                     /gene="phnE"
                     /locus_tag="VF226_19050"
     CDS             5096..5932
                     /gene="phnE"
                     /locus_tag="VF226_19050"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX7076142.1"
                     /translation="MAVASATGGSEFDDRVFAVEHRRRRARWRSASVVALFVLLAAVC
                     LYNILVIDTDWARMGSFGEVLRSIGRFLAIDFELVPHLLEPIIETLMMSMLGTALGAL
                     FCIPVIWLGAHNITPSRWIAYPIGRFAMTLSRSIHEIVWALVFVSAVGLGSLAGILAL
                     AMRSIGFIAKIIAEAIENVDPKPIEAIRAVGGNRLQVLRYGIVPQVLPVAIGVIIFEW
                     DINIRRSAIMGLVGAGGLGLTFHRQMVMFNYPGVTTVIVAILLLIILGEALSYFARKA
                     VI"
     gene            5946..6788
                     /gene="phnE"
                     /locus_tag="VF226_19055"
     CDS             5946..6788
                     /gene="phnE"
                     /locus_tag="VF226_19055"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HEX7076143.1"
                     /translation="MATYGGDGGQPVERRTWSRFRYPDTLIAFAVQLGVLVLLGVSLV
                     YLDVRLERLLEFGQRFGALIVDRYYPPELSLLSEPLYIRAIFDTMQMAYLGTLFGLLL
                     AAPLAWFAAFNLSPNKRFLYPFGRLAIMGCRAVHEMIWTILLVSILGFGMLAGTLALT
                     LFCIGFAGKLMSEEIEAIDKGQVEAIRATGANELQVFAYAVLPQVRVAWTGIAIYTWD
                     VAFRAATVVGFFGAGGMGWYLQRYVQQLESQRVAAVLISIVLLVVISEVVSAWARARV
                     ARAK"
     gene            6992..7321
                     /locus_tag="VF226_19060"
     CDS             6992..7321
                     /locus_tag="VF226_19060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076144.1"
                     /translation="MRKWMIGVLALAGSAFAVVPGAIAQSEWGDDDADWVWNEFDTRT
                     YYTPPTVGMAPPPATVTPGPQPGMSYFSPPTAAPAPEAPAVGPGQCGTYAFWSETLGR
                     CVDARSR"
     gene            complement(7404..7589)
                     /locus_tag="VF226_19065"
     CDS             complement(7404..7589)
                     /locus_tag="VF226_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076145.1"
                     /translation="MGGSSDGSNDSAVAVRAASRVASERARGVGKKRRSGSLALTALI
                     VVAVLGAMLWAMIALAP"
     gene            complement(7621..8061)
                     /locus_tag="VF226_19070"
     CDS             complement(7621..8061)
                     /locus_tag="VF226_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7076146.1"
                     /translation="MRYLHTMVRVSNIDQSLDFYCNKLGLEEVRRVENEAGRYTLIFL
                     AAPGDKDRAVSERAPLIELTYNWDPEEYGSGRNFGHLAYEVDDIYATCDRLMKSGVTI
                     NRPPRDGRMAFIRSPDGISIELLQKGEAQPKQEPWASMPNTGTW"
     gene            8638..9261
                     /locus_tag="VF226_19075"
     CDS             8638..9261
                     /locus_tag="VF226_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7076147.1"
                     /translation="MGDNKLPPDHPDSARQLLDSSGTLDQVQSLDEIGGLDVFEVAGS
                     IKWFDASKGYGFIVPDNGHADVLLHVTCLRAGGYQTAYEGARVHCQVLRRPKGLQAFR
                     ILSMDESTAIHPSQLPQRTHVVVQAESDWERAMVKWFNRVRGFGFLTRGEGTPDIFVH
                     METLRRFGFTELRPGQIVQVRWGHGCKGCMAAELRPDGPVSGLPSQH"
     gene            9350..9772
                     /locus_tag="VF226_19080"
     CDS             9350..9772
                     /locus_tag="VF226_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX7076148.1"
                     /translation="MLVLVPSPDAKMRQERLTIIAATGAHEILVEVAETPEEKSVGLM
                     FRRSVPEGTGMLFPYPAPQEVTMWMRNTYVPLDMIFIRADGVIHRIAERTEPFSERTI
                     ASQGPVTAVLELAGGTAHRLGLKPGDRVIHPHFKSARQ"
     gene            complement(9808..10038)
                     /locus_tag="VF226_19085"
     CDS             complement(9808..10038)
                     /locus_tag="VF226_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076149.1"
                     /translation="MASETRGATAVRVDEGPITLFQLAYAIVLVGAGLLSVAAFTYGL
                     EHSSASGGLLWALIGLVCYLAVWAWGILLAQG"
     gene            10328..10522
                     /locus_tag="VF226_19090"
     CDS             10328..10522
                     /locus_tag="VF226_19090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX7076150.1"
                     /translation="MLEWAFIFLIIALVAGALGFTGVARGAARIAIILFWVFLAIFVV
                     LVILALLGVTAVSTSPPAAG"
     gene            complement(10519..11313)
                     /locus_tag="VF226_19095"
     CDS             complement(10519..11313)
                     /locus_tag="VF226_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076151.1"
                     /translation="MLFLLKITITPVLVALMSLIARRWGPVVGGLIMGLPWMTGPILY
                     FLGLERGEAYAANTAVGVLLGTVGIAGYLVVYALTARRARWPVSLGLASLTYGAIGYI
                     LSGLNLGLWLAAASGVSSLCAAFLLIPRAPDPGGIRYLPWWDIPMRMLATAVLVTIIT
                     FSADHLGPELSGVVATFPVIVSVIGTFTHSQWGWAAVVQLDRGVALSLQSFVVFFLAV
                     GWLAETVGLIWSFLLATAAALLLSAALLASTRRQSKRSREIKATSP"
     gene            complement(11477..11920)
                     /locus_tag="VF226_19100"
     CDS             complement(11477..11920)
                     /locus_tag="VF226_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076152.1"
                     /translation="MASLCPVAAHAETCTREDFEAVVDEAAAALNELNAKHKPAFQAQ
                     LRELKEKRGWDHDTFMREAVPFVQDEQIAKYDQETADYLARISSLGEEGATAVKPDCK
                     LLVEVRATMEGLVAAQKAKWSYMFEKIGQALGTQAGTETSAKQPH"
     gene            complement(12058..14310)
                     /gene="parC"
                     /locus_tag="VF226_19105"
     CDS             complement(12058..14310)
                     /gene="parC"
                     /locus_tag="VF226_19105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX7076153.1"
                     /translation="MTTKPILPGPGPIEPINLKEALEERYLSYALSTIMGRALPDVRD
                     GLKPVHRRILYAMRELRLDPDAAYKKCARVVGDVIGKYHPHGDQAVYDALVRLAQDFA
                     QRYPLVDGQGNFGNIDGDNAAAYRYTEARLSETALRLLEGIDEETVDFRPTFNGEDEE
                     PVVLPANFPNVLANGSSGIAVGMATNIPPHNVEELCNALLHLIKHPNASIEKLVEFIP
                     GPDFPTGGVLVEPRESIIEAYKTGRGGFRLRAKWHREDTGRGTYQVVVTEIPYQVPKS
                     KLVEKIAELLQERKLPLVADVRDESAEEVRLVIEPKSRSIDPVLMMESLFRLTELEIR
                     FGLNMNVLSHGQIPNVLSLRDVLRQWLDHRKEVLIRRSQFRLRKIEHRLEVLDGYLIA
                     YLNIDEVIRIIRFEDEPKEKLIARFGLTDVQAEAILNLRLRALSKLEEIEIKAEHDRL
                     SKERRDLQKLLKSEDLQWSKIADEVRETRDHYSKKTELGRRRTSFAEPPEIEVDLEQA
                     MVEKEPITVILSEKGWIRAMKGHLDDVSKLDFKQGDKLKRAVKAFTTDKLLVLATNGR
                     FFTLSGNELPGGRGHGEPVRLMVDLEENHDVLEIFVHQPGRKLLVAATSGHGFIVPED
                     EVVASTRKGRQVLNVTEPDEAKICVPAEGDMVATVGDNRKMLVFPLDEVHEMNRGKGV
                     ILQRIKGGKLADARVFNKTDGLTWLDAAGRTFTLSMKELGDWVGQRAHAGKAAPKGFP
                     RSNRFGPAFG"
     gene            14561..14875
                     /locus_tag="VF226_19110"
     CDS             14561..14875
                     /locus_tag="VF226_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076154.1"
                     /translation="MFRKFAHPIMALSLMFAIAAAAAQPAEAGRGGRAAAFVGGTLLG
                     LGIAGAIAHGHGPVYYEDYGRCYKGRLRCKWVEGPCYYNKYGDYVCRRGHERCWRPTY
                     CD"
     gene            complement(14949..15716)
                     /gene="rlmB"
                     /locus_tag="VF226_19115"
     CDS             complement(14949..15716)
                     /gene="rlmB"
                     /locus_tag="VF226_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX7076155.1"
                     /translation="MPEPRRDSRREPQDGPVLLYGIHAVEAALRNPRRAIRRLLLTEN
                     AERRLAEAIAARGVQAERVIPRDLDRRLGPDTVHQGALLETEPLPESSLAELASRTDR
                     GPIVVLDQVTDPHNVGAVLRSAAAFGAAGLVMTRRHSPPLAGALAKSASGALDLVPVA
                     LVQNLARSLAELGELGLTRIGLDGTASEAIEDQRLTGPIALVLGAEGKGLRQLTKESC
                     DRLCRITTPGPLSSLNVSNAAAVVLHAAALHRQGRAG"
     gene            15903..15987
                     /locus_tag="VF226_19120"
     tRNA            15903..15987
                     /locus_tag="VF226_19120"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15937..15939,aa:Tyr,seq:gta)
     gene            16014..16087
                     /locus_tag="VF226_19125"
     tRNA            16014..16087
                     /locus_tag="VF226_19125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16046..16048,aa:Gly,seq:tcc)
     gene            16271..>16407
                     /locus_tag="VF226_19130"
     CDS             16271..>16407
                     /locus_tag="VF226_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7076156.1"
                     /translation="MAKQKFERTKPHVNVGTIGHVDHGKTTLTAALTKVSADQGWTTE
                     AV"
BASE COUNT         2894 a         5089 c         5285 g         3139 t
ORIGIN      
        1 ctcgatggtg atgttggcgc cctcgcgctg ggcgatggcc tgcagcttct cgcgctcttg
       61 cagggctcgc cttttcgccg ccggccagac ctcgttcaag agcgcccgca cattggcggc
      121 cgtcttggcc atggtgttgt cgagcttgta gtcggcgaag gtttcgtagc cgagcagcct
      181 tgcccgttcg cgccgcagct tcagcatttc cgcgatgatg ccgcgattat cggttgctcc
      241 gccgttctcg ccgcggcggg tccaggccgt gaacgccttc tcacgcaggt cgcggcgcgg
      301 ggagaacgtc agaaacggct cgatcagcga tcgggacagc gtgatgacga acttggccgc
      361 actgccgcgc tcctcggccg tcctgcgggc ggcatcgatc aggaagtccg gcaggccggc
      421 gagatcctcc tctgtgagcg gcagctcgta gctcttctcg tcagcgagga cgttctggct
      481 gaattgcgtg ccgagggcgg ccagccgctc catgatttcg cccagacgct tcttctcctc
      541 gggcccgagc cgcgcacccg acctcacgaa cgcgaggtgc gtccgttcca gaactcgcct
      601 ctgctcgggg ttgagatccg ccgactccgc gccctgcatt acggcatcga tgcgcgcgaa
      661 cagagctgcg ttcgaggtga tcgcattcca gtgctgagcg agcttcggcg agatctcgcg
      721 ctcgatagct tgcagcgcgt cgttggtatc cgcgctggca aggttccaga aaacggcaga
      781 aacgcggtcc aggaggcgac cggcgttctc gagcgccgcg atcgtgttgt cgaaagtggg
      841 gggcgctggg tcgccggcaa tgcggtcgat ctccgagcgg tgctcggcca aggctcgctc
      901 gaacgccggc cggaagtgct ccggcgcgat ctcggcgaac ggcggagcgc cgaacggcgt
      961 ggaccagggg acgagcaaag gattggctgc cggactggcg ctcatggtgc agggacctcg
     1021 ggggatctgg atcgtgcgat ttcgagaacc gtatagttac tgcaacgtaa agtggaagca
     1081 acctctaaac acgcgctccc aacccgctgg ttttacgtcc tttctctctc cggcctgact
     1141 tgccgggtgg ctgacgtatc aaggcgcgcc cagtctcggt cacattgcgt cgagcggcaa
     1201 gcaagctctc cacgacgccg aaaaaggcgc gcacgagggg accctgccgg cgctctgcga
     1261 ggcagaccac gtgggcgtac gtgtagattt cggcatcccg gaacgggagt ggatgcaaac
     1321 gagggtgcgg gatcagctcg aactccgaca cgatgccgac gcccatgccg cgctcgactg
     1381 ccagccagat cgcctcgcgg ctgccgattt cagtgaagct gcccatgcgg gcgccggcgc
     1441 tggccagacc cgcctcgaac gccagccgcg tcgtcgagcc gacctcgcgc agaatgacgt
     1501 gctgttccgc gaactcgtgg atggagaccg aggtgcgccc ggcccaagga tgatcggaat
     1561 tgacgaaggc gatcaccgga tgccggctgt aggggacagc gtggattcgc gggtcgtctt
     1621 ccgtatgggc caggaccgcg acgtcggcct tgtagtccat gagctgccgc agcgcctcgc
     1681 gggaattgct gatgcgtacg gagaattcga cgccgggata ccgatcccgg aaggcgctca
     1741 gcatctccgt gacgtgatac ggcccgacgg cacttactct cagatggccg gagcgtagcc
     1801 cgcccacggc attcaacaga tcgacggctt cgctttccag gctggcgatc cgctgcgtca
     1861 gctccagcaa gcggcgaccg gtttcggtga gctcgacgcg gcggccccgc ctatgaaaca
     1921 gctcgactct gtagcgctcc tccagcgctt tcacctgact gctgatcgtc ggctgtccga
     1981 tgttgagcac gttggcggca gcagtgaatc cgccgtgcgt tgcgaccgtg tgaaaggagc
     2041 ggagctgggt atccatctat cgatccaatg aatgggagtc atggcgaatt tggattggat
     2101 caatgatacg gtccgtgaca acctgtacgc aataacgagc ggcaggggct tgacgtgtgg
     2161 acgtatcaca acccggttcg catcctgttc ggtgagggaa cgttcgataa gctcgggagt
     2221 ctgatcgccg gccggcgcta cgtgctcgtc acctacggcg agccgctgtt ccgtgaactt
     2281 gcggcaagac tcgctcgatc agccggcgcc cccgccctcg tcgtcgacga tgtcgcgccg
     2341 aacccggacc gtgccgatct cagccggcaa tgccagcgat ggaacgagct ggcggaggcg
     2401 ccgcaactcg tcgttgccgt cggcggcgga tcggttatcg atacggccaa ggtgatcgcc
     2461 gctgcacgcg ggaacttcgg gcccgtgtgg gaagtgctga tcaccggtcg cggcgccgaa
     2521 cggctcgagc cccttccgat catcgctgtg ccgacgacgg cgggaacggg aagcgaagtg
     2581 acgtcgtggg cgacggtgtg ggacggcgaa ggtggccgta agtattcact cgcagcgcat
     2641 gcactgcatc cggtccatgc cgtcctcgat cccgagctta ccttgagctg ccccaagggg
     2701 ctcacgatca gcaccgggtt ggacgcgctc tcacatgctc tggagagcat ctggaatcgc
     2761 aacgccaatc cggtctctgc tgttcacgcc gtggccgcgg cgcgggaaat cctggaggtg
     2821 ctgccggctc tgtccctgcg gctcgaagat cgcgcgctac gcgcacgcat gctgcgggcg
     2881 gccatgttct cgggcctcgc attctcgaac accaagacgg caatcgccca ctcgatgtcc
     2941 tatgcggtga cgctgcgcca tggaacggtt catggcattg cctgctcgtt cacgctaccc
     3001 atggtcttgc gcagcgtcat cggcctcaat gctgattgcg acgcggtgct tgcgaggatc
     3061 ttcggcgcgg atctcgctgc cggagcggcc aggctgcagg gctttctgga ggacttgggc
     3121 gtctcgacgc gcccggccga tcacggcgtc ggcggcgagg agtggcgctc gatcgtcgaa
     3181 gatgcgctcg ggggtgagcg aggattgaac ttcctaggca gcaaagaggg cttgctggca
     3241 caaatctgag caacaacgac tgtcacagag cttctacgca aggaggccat agaaggaccg
     3301 gtgtctcgca ggcgatctgc gagtgcggga aacaagcagt aaatggagga acgtcatgaa
     3361 gcttcgaact ttatttgccg ccgcgatcag cgccctgttg gccatgacgg gcgctgcttc
     3421 tgcacaggaa aagtgcgaga atccagaagc cctgcgcttc tcgatcattc cgaccgaaga
     3481 gaccatgcag gagctggcgc tctacaagcc ggtgatcgat attctatcga ggaatacggg
     3541 caaaaagatc gagttctaca tgccgacgtc ctacgcctcg gtgatcgagg cgatgctcgg
     3601 caactgggtc gacatcgccg tgcatgggcc gaactcgtac gtgatcggca acgagaagag
     3661 caagaacatc gaagtttttg cgacttatgc gaaagcgaag ggcattttcc aggaggaggg
     3721 gcccgggtat cacgcaatcc tcatcgtcag gaaggacagc aagttcaaag acatcgcatc
     3781 tctcaaaggg gctgtggttg cgctcgccga tccggcttcg acatctggca acttgatgcc
     3841 gcgggtggag tttgccaagg agatcggcgg tggcgagctg gaaagctatt tttccaaagt
     3901 cgtttatagc ggtagccatg ataagtccgc ggtggccgtg agcgagggta aggcggatgc
     3961 ggcattcgtc gcgacgcacc ggctcgacaa cgtcatcgag cgcgggctcg tcaaggccga
     4021 ggattttgcg gtgctctgga agtcgcgcct cgtgccgcag gatccattca cctatcgcgg
     4081 cacgctctgt gaggatttga aggccaagat ccgggatact ttcctcaacc tgcataacga
     4141 gcccggtgcc aagaaatttc tcgaaaacct caactccgtg aaattcgtgg cgatgaagtc
     4201 cgaggattac gatctgatcc gggatctgcg ggctgcccag gcgaagaaga aataggagcc
     4261 ctgggcggtt gccggagcgg cgggtcaacg cgcctgccgc cccttccgtt tagactccaa
     4321 ctgatggaag cacgaacgca gtgcctatct tagacgtcca ggacctcagc gtacgctact
     4381 cgcgcaatgg agccgagatc ctcaaagggg tcagcctcca ggtcgatgcc ggcgacttct
     4441 gtgccgtcat cggtccgagc ggcgcaggca aatcgactct gatccgctgc atcaatcgcc
     4501 ttgtcgagcc gagggccggc cgtatcttgc tctacggcga ggacgtggtc accatgtcca
     4561 cccgccggct gcgtgaaacc cggcgtcaca tcggcatgat cttccaggaa ttcaacctgg
     4621 tgaaccgcat gtcggtgatg gataacgtcc tgtcgggccg gcttggctcg gccgggacct
     4681 tgcgcagcct gctgaagctg ttcagccgcg aggatatcga gcgcgcattg gcactgttgg
     4741 accgggtggg cctttccgat cacgtcgaca agagggcgga tcagctctcc ggtggtcagc
     4801 gtcagcgcgt cggcatcgcc cgggcattga tgcaggatcc caagttgctg ctcgtcgacg
     4861 agccgacctc gagcctcgat cccaagatcg cccgcgaggt gatgaccctc atccgtgacg
     4921 tggcccggga gttcaacgtg ccggcgctgg tcaatatcca cgacgtgcag ctcgcgaccg
     4981 agttctgcaa taagatcatc gggctgcagg acggggtgaa gatgttcgac ggcccgaccg
     5041 agcagctcga ccgcaagatg ctcgacacga tctacgcgat ggaggtgctg tgaccatggc
     5101 cgtcgcgagt gcaaccggcg gcagcgagtt cgatgaccgc gtatttgccg tggagcaccg
     5161 gcgccgaaga gcacgctggc gatccgcttc tgttgtcgct ctgtttgtgc ttctcgccgc
     5221 ggtctgcctt tacaatattc tcgtcatcga caccgattgg gcccggatgg gctctttcgg
     5281 ggaggtgctg cggtccatag gccggtttct ggcgatcgat ttcgagctcg tccctcacct
     5341 tctcgagccg atcatcgaga cgctgatgat gtcgatgctc ggcaccgcgc tcggcgccct
     5401 tttctgcatc cccgtcatct ggcttggcgc ccacaacatt acgccgtcgc gctggatcgc
     5461 ctatccgatc ggtcgcttcg cgatgacgct cagccggtcg atccacgaga tcgtctgggc
     5521 actggtgttc gtgtctgctg tcgggctcgg gtcgctcgcg ggcatccttg cccttgccat
     5581 gcggtccatc ggcttcattg caaagatcat cgctgaagct atcgagaacg tggatccgaa
     5641 gccgatcgag gccattcgcg ccgtaggcgg aaatcgcctg caggtgctgc gctatggcat
     5701 cgtcccgcag gtcctcccgg ttgccatcgg cgtgatcatc ttcgagtggg atatcaacat
     5761 ccggcgctcc gccatcatgg gtctcgtcgg tgccggtggt ctcggcctga cgttccatcg
     5821 gcagatggtg atgttcaact acccaggtgt caccactgtc atcgtggcga tcctgctgct
     5881 catcatcctg ggcgaagcgc tgtcttattt cgcacgcaag gcggtcatct gacgatatgc
     5941 gggacgtggc gacatacgga ggagatggcg ggcagccggt cgaacggcgg acatggtcgc
     6001 gtttccgcta tccggatacg ctcatcgcgt ttgcagtcca gctcggcgtt ctcgtccttc
     6061 tcggcgtttc gctcgtctat ctggacgtgc ggctcgagcg attgctcgag ttcggccagc
     6121 gattcggcgc gctcatcgtc gatcgctatt atcctcctga gctcagcctg ctcagcgagc
     6181 cgctctatat ccgggccatt ttcgacacga tgcagatggc ctatctgggc actttgttcg
     6241 gtctgctgct cgccgcgccg ctcgcctggt tcgctgcatt caatctgtcc ccgaacaagc
     6301 gctttctcta tccgtttggc cggcttgcga tcatgggttg ccgggccgtg cacgagatga
     6361 tctggacgat cctgctcgtc agcattctcg gattcggcat gctcgccggc acgctggcgc
     6421 tgaccctctt ttgcattggc tttgccggaa agctgatgtc ggaagaaatc gaggccatcg
     6481 acaagggaca ggtcgaggcc atccgcgcaa cgggcgcgaa cgagctccag gtcttcgctt
     6541 acgccgtgtt gccgcaagtg cgcgtcgcct ggacgggcat tgccatctac acctgggatg
     6601 ttgctttccg ggcagcgaca gtcgtcggat ttttcggggc cggcggcatg ggctggtatc
     6661 tccagcgcta cgtccagcag ctcgaaagcc agcgcgtggc ggcagttctc atttccatcg
     6721 tgctgcttgt ggtgatttcc gaggtggtat cggcctgggc acgggcacgc gtcgcccggg
     6781 cgaaatagcg tgccggcgcg gcgcggctcc tagctcccag tccacccgca tgccatgcaa
     6841 gccggcgctt cctccgacga cgcaggtctc acttaatcgg cagcggaccg cttcttcgca
     6901 tgctcgcgtc atctgatctc cgccttgaat gagcgcggaa ccactcctct gagctgtcgt
     6961 taactggcga ctcaaagagg aggttactca gatgcggaaa tggatgatcg gcgtgctggc
     7021 cctggccggg tcggcatttg cggttgtgcc tggagccatc gcgcaaagcg agtggggtga
     7081 cgatgacgcc gactgggtgt ggaacgagtt tgatacccgg acctattaca caccgccgac
     7141 ggtcggcatg gcgccgccgc cggccacggt aacgcctggg ccacagcccg gcatgtcgta
     7201 tttcagcccg ccgaccgctg caccggcacc ggaagctccg gccgtcggtc ctggtcaatg
     7261 cggcacatac gccttttggt cggagacact gggccgctgc gtggatgcgc gcagccggta
     7321 gattgcatcc gccaaagccc gaggcgacgg caggatattg ccccgcctcg gctgccgact
     7381 tgctacgacg acggtagccc tcgtcagggc gccagcgcaa tcatagccca aagcatggcg
     7441 ccgagcacgg cgacgacgat gagcgccgtc agcgccaacg agcctgagcg gcgttttttc
     7501 cctacgccac gcgcccgctc agacgcgacc cgggaagcgg cgcgcacggc gaccgccgaa
     7561 tcgttcgacc catcgctcga cccacccaca attggccccc ttcggcctta tggttgtccc
     7621 ctaccaggta cccgtattcg gcatcgaggc ccacggctct tgcttcggtt gagcttcgcc
     7681 cttctgcaac agctcgattg agatgccgtc cggtgatcgg atgaaggcca tgcgcccgtc
     7741 cctgggaggc cgattgatcg tcaccccgga tttcatcagc cggtcgcagg tcgcatagat
     7801 gtcgtcgacc tcgtaggcga ggtggccgaa gttgcggccc gacccgtact cctcgggatc
     7861 ccaattgtac gtgagctcga tgagcggcgc acgctcgctt acggcccggt ccttgtctcc
     7921 cggagcggcg aggaagatca gcgtgtagcg cccggcctcg ttctccaccc gccggacctc
     7981 ctccaggccc agcttgttgc agtagaaatc caggctctgg tcgatgttgc tgactcgcac
     8041 catagtgtga agatatcgca tgtcgcctcc gctctgggtg agtgccgctc ccgttgacat
     8101 acgctgcgca aatggcgcgc acaagcctgt cctgccaacg gtttccgtct tgtcaagccg
     8161 tcgatacggc gcagaacggc cgccgatgtc agcgactgct cgcacaaatc ggggaaagcc
     8221 agtggacgca ttgttggtcg aacgacgggc gagagcgacg gaagtggcct cgcggccgca
     8281 ggtttcgacg cgaatcactc cgcactgatt cgccgtgcac aaagccatca acagctcgtt
     8341 cgaagccgtc cgaggcggta tgacacccac agggaaaacg atgggataga gaggaattcg
     8401 taagaaacaa ttctggagga atatcatcgt attacggatg tgtattgaaa cggaatgtgt
     8461 gcttcgtatc cccgggcaga tgttaatcga tggtaaattc cttatggccc tcgcgggctg
     8521 cgatggtaca ttttgcaacg cttgccagtg ccgagtcgcc ggagagggcc tctgacgcga
     8581 ttcgctcggc aggcgcatca agtaggtcga cgtaaaccca agtagcgggg tgtgctaatg
     8641 ggggataaca aactacctcc cgatcatccg gattcggctc gacaactgct cgacagcagc
     8701 ggcactctcg atcaggtcca gtccctggat gaaatcggtg gcctcgacgt gttcgaggtt
     8761 gccgggtcaa tcaagtggtt cgatgcatcc aagggctacg gcttcatcgt tcccgacaac
     8821 ggccacgcag acgtgctcct gcacgtcacc tgcctgaggg ccggcggcta ccagaccgcg
     8881 tacgaggggg cccgcgtgca ctgtcaggtg ttgcgccgtc ccaagggtct gcaagctttc
     8941 cgcatcctca gcatggatga gagcacggcc atccatccct cccagctccc gcagcgtacc
     9001 catgtcgtgg tacaagcgga gagtgactgg gagcgggcga tggtgaagtg gttcaaccgg
     9061 gtccgcgggt ttggctttct gacgcgtgga gagggtaccc ctgacatttt cgtccacatg
     9121 gagacgctgc gccggtttgg attcactgag ctcaggccgg gtcagatcgt gcaggtccgg
     9181 tggggacacg gctgcaaggg ctgcatggcg gccgagctgc ggccggatgg gcctgtttcg
     9241 ggcctcccgt cccagcattg agaggttgcc aattctgcca atgtgcgcca ttgcggacag
     9301 gaccgctctt cgggcggtcc tgtttgcttt cttgtccgca gtggttgcaa tgctcgtcct
     9361 cgtcccctct cccgacgcca agatgcgcca ggaacgcctc accatcatag cggcgaccgg
     9421 cgcgcacgag atcttggtgg aggttgcgga aacgcccgag gagaagtcgg tcgggctgat
     9481 gttccggcga agtgttccgg aggggacggg gatgctgttc ccctatccgg cgccgcagga
     9541 agtgacgatg tggatgcgga acacctatgt gccgctcgac atgatcttca tccgcgctga
     9601 cggtgtgatt caccgcatag ccgagcggac ggagcccttt tccgagcgca cgatcgcatc
     9661 acaggggccg gtgacggccg tcctggagct ggccggaggg acagctcatc gcctcggcct
     9721 gaagcctggc gacagggtca tccatccgca cttcaagtcg gcacgccagt gaggatcagc
     9781 ggctgacctt cgtcgcgcga aggactatta gccctgtgcc agcagaatgc cccaggccca
     9841 tacggcgagg tagcatacga ggccgatgag cgcccaaagc aagccgccag aggccgagct
     9901 atgctccagc ccataggtga acgcggccac cgaaagcaga cctgccccga ccaggacaat
     9961 ggcataggca agctgaaata gggtaatggg cccttcgtcg acacgaacgg cagttgctcc
    10021 gcgagtttcc gaggccatct gatccctccg gcatcgagag gtgcagtcgc cagctttatg
    10081 gtgccgcgtc gcaggcctat gtcagccgca tcgttcacgg ccccacgtca gcataactcg
    10141 aatcgccagc aagtcgatac gcaggagctc cgagatcgct gatgcgatcc gtgtgccgcg
    10201 ccagggagcg cacgttacgg tctggtgcgg tgtctccgcc gacgccccgg ggaaccgggc
    10261 ttggtcggga ccgttctgct gctgtcactt cgatcgggcg cgcgacccag aacggagccg
    10321 atgccgtatg ctggaatggg ccttcatttt tctgatcatc gcgctggttg ccggagcact
    10381 cggcttcact ggcgtcgcgc gcggagcagc gcgcatcgcg atcatcctgt tctgggtttt
    10441 cctcgcgatc ttcgtcgttc tcgtcatact ggcgctgctc ggggttactg cagtctccac
    10501 cagtcctccc gctgccggct aagggctggt cgccttgatc tcccgggacc gtttgctctg
    10561 gcgtcgtgtc gaggcgagca gggctgcgct gaggagaagc gccgcggcgg tggccagcag
    10621 gaatgaccag atcagtccca cggtttcggc cagccatccg acagccagga agaagacgac
    10681 gaaagactgc agcgacagcg cgacgccgcg gtcaagctga acgacggcgg cccagcccca
    10741 ttgcgaatgc gtgaaggtgc cgatgaccga tacgatgaca gggaaggtcg ccaccacgcc
    10801 gctgagctcg ggtccgagat ggtcggcgga gaaggtgatg atggtcacga ggaccgccgt
    10861 ggcaagcatt ctcatcggaa tgtcccacca gggcaggtag cgaataccac cagggtctgg
    10921 cgcgcgcggg atcaggagga aagctgcgca caggctcgaa acaccgctcg ccgcagcgag
    10981 ccagaggcca aggttgaggc cgctgagtat atatccgatg gctccatagg tgagagaagc
    11041 gagaccgagg ctgaccggcc agcgggcacg gcgggccgtg agagcgtaga ccacgaggta
    11101 accagcgatg ccgacggtgc cgaggaggac acccaccgct gtgtttgctg cgtaggcctc
    11161 cccgcgctcg agcccgagaa agtacaggat cggccctgtc atccacggca ggcccatgat
    11221 caagccgccg acgaccggcc cccagcggcg cgcgataagg ctcatcagcg ccacgagcac
    11281 cggcgtaatg gtgatcttca gcaggaaaag catctgaggc ccgctaggcg cttggaagta
    11341 cgaggggtca cggctccgca ggagcgagga gcaagggcgg catagcttcg tcggatggct
    11401 cttgcaagtt gggcgcaacc gccgagcacc ggtctgccgg taaccggtgt cggcgcgtcg
    11461 gttctgccag cgtagcctag tgtggctgct tggcgctcgt ttcggtgccg gcttgggttc
    11521 cgagcgcctg tccgatcttc tcgaacatgt agctccattt ggccttttgc gcggcaacga
    11581 gaccttccat ggtcgcgcgc acctcgacga gcagcttgca atccggcttg accgcggttg
    11641 cgccttcctc gcccaggctg ctgatacggg cgagataatc ggccgtttcc tgatcatact
    11701 tggcgatctg ctcgtcctgc acgaaaggga cggcctcgcg catgaaagtg tcgtgatccc
    11761 agccgcgctt ctccttcagc tcgcgaagct gggcctgaaa cgccggcttg tgcttggcgt
    11821 tgagctcgtt gagtgctgcg gccgcctcgt caacgaccgc ctcgaaatcc tcccgcgtgc
    11881 aggtttccgc gtgcgcggcg accgggcaca gactggccat cagcaaagca atcgcaaccg
    11941 agcgccacat ctggctgaac accccgtcgc cggcgagcag acgttaccca tggcccagag
    12001 caggccgaag cgcaagtgcc tcatcggcac tctgatgaac ccttgttccg gttgcgatca
    12061 gccgaaagca gggccgaacc tgttcgagcg cgggaacccc ttgggcgcgg ccttgccggc
    12121 gtgcgcacgc tgacccaccc agtcgccgag ctccttcatg ctcagcgtga aggtacgccc
    12181 ggcggcatcg agccaggtca gcccgtcagt cttattgaaa acgcgggcat cggccagctt
    12241 gccgcccttg atccgctgca ggatcacgcc ctttccgcgg ttcatctcgt ggacctcgtc
    12301 gagcgggaaa acgagcatct tccgattgtc gccgacggtg gcgaccatgt cgccttccgc
    12361 cggaacgcag atcttcgcct cgtcgggctc ggtgacgttc aagacctggc ggcccttgcg
    12421 ggtggaggcg accacctcgt cctcaggcac gatgaagcca tgtccgctgg tcgcagcgac
    12481 aagcagcttg cgcccgggct gatggacgaa gatttcgagc acatcgtggt tttcctcgag
    12541 gtcgaccatc agccgaaccg gctcgccatg gccgcggccg cccggcagct cgttgcccga
    12601 gagcgtgaag aagcgcccgt tggtcgcaag gacgagcagc ttgtcggtgg tgaaggcctt
    12661 cacggcgcgt ttcagcttgt cgccctgctt gaagtcgagc ttcgagacat cgtcgagatg
    12721 ccccttcatc gcgcggatcc agcccttctc cgagagaatg accgtgatcg gctctttctc
    12781 gaccatcgcc tgctcgagat ccacctcgat ctccggtggc tcggcgaacg aggtgcggcg
    12841 gcggccgagc tcggtcttct tggaatagtg atcgcgcgtc tcgcgcacct catccgcgat
    12901 tttcgaccac tgcaggtcct cggacttgag aagcttctgc aggtcgcgcc gttccttcga
    12961 cagcctgtcg tgctcggcct tgatctcgat ctcctcgagc ttggacagcg cgcgcaagcg
    13021 caggttcagg atcgcttcgg cctgcacatc cgtgagcccg aacctggcaa tgagcttctc
    13081 cttcggctcg tcctcgaacc gaatgatccg gatcacctcg tcgatgttga ggtacgcgat
    13141 gagatagccg tcgagcactt ccagccggtg ctcgatcttc ctgaggcgga actgcgagcg
    13201 ccggatcagc acctctttgc ggtgatccag ccactgccgc agcacgtccc gcaacgacag
    13261 gacgttcggg atctggccgt gcgagagcac gttcatgttg agaccgaagc ggatctcgag
    13321 ctcggtgagc cggaacaggc tctccatcat cagcacggga tcgatgctcc ggctctttgg
    13381 ctcgatgacg aggcggacct cctcggccga ttcgtcacgg acgtcggcca ccaaaggcag
    13441 cttccgctcc tgcagcagct cggcgatctt ctcgacgagc ttggatttcg gcacctggta
    13501 ggggatctcg gtaacgacga cctgataggt gccccggccg gtatcctcgc gatgccactt
    13561 ggcacgcagc cggaacccgc cgcggccggt cttgtaggct tcgatgattg actcacgcgg
    13621 ctcgaccaac acgccgcccg tcgggaaatc cggcccgggg atgaactcga ccagcttctc
    13681 gatcgaggca ttcgggtgct tgatgaggtg caggagcgcg ttgcacagct cctcgacgtt
    13741 gtgcggcgga atgttggtcg ccatgccaac ggcgatgccc gacgagccgt tcgccaggac
    13801 attggggaag ttggccggca ggacgacggg ctcctcgtcc tcgccgttga aggtcgggcg
    13861 gaagtcgacc gtctcctcgt cgatgccctc gagcagccga agggccgttt cggagagccg
    13921 cgcctcggtg tagcggtagg ccgcagcgtt gtcgccgtcg atgttgccga agttgccctg
    13981 cccatcgacc agtggataac gctgggcgaa gtcctgcgcg agcctgacca gcgcatcgta
    14041 gaccgcctgg tcgccgtgcg ggtggtactt accgatgacg tcgccgacca cgcgggcgca
    14101 cttcttgtac gcggcatcgg ggtcgagccg cagctcgcgc atggcgtaga ggatacgccg
    14161 gtgcaccggt ttcaggccgt cacgcacgtc cggcagcgcg cggcccatga tggtggacag
    14221 cgcataagac aggtagcgct cctcgagcgc ctccttgaga ttgatgggct cgatggggcc
    14281 cgggccgggc agaatcggtt ttgtggtcat gaaagctcgt tagcggagcg cctattcggc
    14341 ctcaagtgcc gcagagccac accctgttgc gcggctgcag gcgcatcccg ttcttcaatt
    14401 ttgatccgct cgagccagat ttgggacgcg ttcacgtcaa attcatctgg caggcgatag
    14461 gaagctgacc gattcgtcaa gaatcgaacg cgccgggctg cttccggcag ccagtcatct
    14521 tgcccgactg ggcggtcaac ttcgcgagat ggtgatcacc atgttcagga agttcgctca
    14581 tccgatcatg gcgctgtcgc tgatgtttgc cattgcggcc gccgctgcgc aaccggccga
    14641 agccggccgc ggagggcgcg ccgcagcctt cgttggcggc acgctcttgg gcctcggtat
    14701 cgccggcgcc atcgcgcacg ggcatgggcc cgtctactac gaggattacg gccggtgcta
    14761 caaaggccgc ctgcgctgca agtgggtcga gggaccgtgc tactacaaca agtacggtga
    14821 ttacgtttgc cgccgcggcc acgagaggtg ctggcggccg acgtactgcg attgagctct
    14881 cgtcgggctg gagcatcggg cgccgcaccc cacggtcggc gccgtaaatt tgatgcttgg
    14941 gcacagcttc agcccgctcg gccctgccgg tgcagagccg cggcgtgaag tacgacggca
    15001 gccgcgttgg agacgttcag gctcgacagc ggccctggcg tggttatccg gcacaggcga
    15061 tcgcagcttt ccttcgtaag ctgacgcaga cccttcccct cggctccaag gacgagggcg
    15121 atcggcccgg tcagccgctg gtcctcgatc gcttccgaag cggtgccgtc gaggccgata
    15181 cgcgtcaagc cgagctctcc cagttctgcc agagaccgtg ccaaattctg gaccagcgcc
    15241 accggcacga gatcgagcgc gcccgaggcg ctcttggcaa gcgcgcccgc gagcggcggg
    15301 ctgtggcggc gagtcatgac gaggccagcg gcaccgaatg ccgcagcaga gcgcaggacc
    15361 gccccgacgt tgtggggatc ggtgacctgg tcgagcacga caataggccc tcgatcggtt
    15421 ctggatgcca gctccgcgag ggaggactcc ggcagcggtt ccgtctcgag cagggcgccc
    15481 tgatgcacgg tgtcaggccc caggcggcgg tcgaggtcgc gggggatcac gcgctcggcc
    15541 tgcaccccac gcgcggcgat ggcctctgcg agccggcgct cggcgttctc cgtaaggagc
    15601 agccgacgga ttgcgcgcct ggggttgcgc agagccgctt ccacggcgtg aattccatag
    15661 agcaggacag gaccgtcctg cggttcgcgc cggctatccc gacgaggctc tggcataatc
    15721 ccgtctcagt gcttacctga cttgcatgat ctgctttccc gcggtatgtc gggcgggccg
    15781 ccggaagcta cgcgcaacgc tacgtttttc aattgacatt tggcacagcc atcacgataa
    15841 agcgcagcgt tgccctgctg gagacggcgg ggcaatttcg tttgactcca tggccgggtc
    15901 ccggaggggt gcccgagtgg ctaaagggga cggactgtaa atccgttggc tatgcctacg
    15961 ctggttcgaa tccagccccc tccaccagct cggacgtggg tcgtgagtcg ggtgcgggtg
    16021 tagctcaatg gtagagcagc agccttccaa gctgaatacg ggggttcgat tcccctcacc
    16081 cgctccattt tcttcgcaat acgcgggcga tgccgtgagg ttttgctgcg gagagcgtgc
    16141 gaaccgtgtc gcagcacggc ggctctggtg agctgaatca aggttcaaag tgttcttgag
    16201 aacgcatgat gacgcggtgg tgcgcaacag gcgcgagtta gggttctgag tagaggcagg
    16261 caggcaaccg atggccaaac aaaaatttga gcggacgaag ccgcacgtga acgtgggcac
    16321 gatcggtcac gttgaccatg ggaagacgac gctgacggcg gctctgacga aggtctcagc
    16381 ggatcagggc tggacgacgg aggcggt
//
LOCUS       DASVDU010000286         6403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_605988, whole genome shotgun sequence.
ACCESSION   DASVDU010000286 DASVDU010000000
VERSION     DASVDU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605988"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..368)
                     /locus_tag="VF226_19135"
     tRNA            complement(292..368)
                     /locus_tag="VF226_19135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(332..334),aa:Arg,seq:tct)
     gene            complement(418..723)
                     /locus_tag="VF226_19140"
     CDS             complement(418..723)
                     /locus_tag="VF226_19140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX7076157.1"
                     /translation="MVARIYKPARSAMQQGTARTRDWVLEFEPQRPRTIDPLMGWTGS
                     QDPREQVQLQFETKEEAIAYAERNGIPYTVQDPDPRRPVPKSYADNFRYGRIGAWTH"
     gene            934..1248
                     /locus_tag="VF226_19145"
     CDS             934..1248
                     /locus_tag="VF226_19145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX7076158.1"
                     /translation="MIVEERDYRVKAGKLGQFVSLYEQHGLPIQLELLGKFIGYFTTE
                     LGELNHVVALWSYDSLDDRQQRRDRMMADPRWQAYLQMVDGLLDVQTTRILRPTSFSP
                     LK"
     gene            1269..2069
                     /locus_tag="VF226_19150"
     CDS             1269..2069
                     /locus_tag="VF226_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7076159.1"
                     /translation="MSTAMVNLPDGAGLAVTTSGAGRDLVLVTGLGGTAAFWDPVVPL
                     LEPHFRVTRFDQRGIGASDRGTAEVSIGLLAEDTRAVLEHLDARDAVLLGHSTGGVIL
                     QELALRDSSRVGGLVLSGTWAKPNRYMTELFRSRSVLLEKAPQEYAAMGAFLGYTPDW
                     LEANWTRFEAVVAAAPTSRAAQKVVAERIAALLAFDRSKDVGSISPPVLIQGAEDDLI
                     VPAFLQRELAELIPQAKLDILPSGGHFFPLTRPAEFLKILTRWTGTLD"
     gene            complement(2141..3484)
                     /locus_tag="VF226_19155"
     CDS             complement(2141..3484)
                     /locus_tag="VF226_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7076160.1"
                     /translation="MPNGLLILMMAGLFLGLGYLGVPVAFAIMAGVFFATSFTPVSLS
                     SIVGQLFHGIDSEALLAVPFFLLVGELMSSARITSQLITLAQTMVGHLRGGLAQVVTL
                     FSMFFAGISGSSTADVAVLARTIGPEMDREGYDRAFTAALIAAASTIANMIPPSIMAV
                     IYGATGNVSIGGLFLGGIAPGIMVGVGLMIYSYFVGPVGVLRPRASFGQFSDAVKSSA
                     LPLMIPIIIMGGILSGFFTPTEAGLIAVAYIVFVLIPLVQRGHIRNLPRDFANAGLLY
                     SIPIAAIAAASAFGWMLAYLKAPDVVAGWIADFAGPNPLVIMFLLVALFVIIGDFIDA
                     VPAIIIFMPIISALTELGNVNPVHMGVVIVVTLAFGLITPPYGLSLLMSSKFVGVSFM
                     TAVVRSLPTYLIFFLTITFCIFFPEVVLWLPKMLLPESVGCFKSPAGTGYICPQL"
     gene            complement(3477..4016)
                     /locus_tag="VF226_19160"
     CDS             complement(3477..4016)
                     /locus_tag="VF226_19160"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7076161.1"
                     /translation="MASLVAPQERHLKWPALDWLEALLFIACGVCIVGFSVSVLCDVI
                     TREIGHPWLWLQQATTGFFIHGSFLGMALAARRNEHMYLAEIVQTMSGNKRRNLEVFS
                     RLVVLVVALCMVVFGYQNFLLDMGSFRMPSLIPLGYYSISVPISGALIALFQVEQIVN
                     GLKNGFPVPLDSRQESSDA"
     gene            complement(4102..5112)
                     /locus_tag="VF226_19165"
     CDS             complement(4102..5112)
                     /locus_tag="VF226_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7076162.1"
                     /translation="MQISKRLLILGSAIAGAAIALLAGTDSYAQERKFSFGYDQPKST
                     GYGLAADIFNAKLMEVSGGKLGIDQFPGAQLGQEPVMLQKLRSGDIDFIITSSANAAT
                     VAPESGVMSLHYLFRDEDHLKKAIADPKVIEAVKTMISDSVQGAHVLCIETLGLRNMY
                     AKREFKKVEDLKGVKVRVQATKTEDTHFPAYGAQTVHMPFGEVYTSLQTGVVEAAENG
                     INVYMSNKHYEVAPIMSLTEHEANNGFIWVSDKTWNSLNDDEKKWVQAAADEVARVQP
                     AKSLQLEKDSAAKLEKLGVKIVRDVDKSGFMAAAKPIQDQLAKELGPHAVKILELIRN
                     VN"
     gene            5296..6081
                     /locus_tag="VF226_19170"
     CDS             5296..6081
                     /locus_tag="VF226_19170"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7076163.1"
                     /translation="MAADPVLCSIEDGVALVTLNNPPLNLVTLELTRQLNSLVTKLAV
                     DPSVRVMVLTGSGSKAFCAGSDIKEFPDMMAAGAVVPKKLALENEAYSRVDDFPKPTI
                     AALNGLAFGGGLELAVCCDLVVGEAGNQVALPEIKLGVFPGSGGTIRVTRRIGEARAK
                     EMMFFGDPITAETALAWGLINRVVPKGEALKTALDMARRLAAQPNVALQMCKQSVDMA
                     FDTAEEAAVRASLALSDKVFASEDCSEGVRAFFAKEQPKFKHR"
     gene            complement(6068..>6403)
                     /locus_tag="VF226_19175"
     CDS             complement(6068..>6403)
                     /locus_tag="VF226_19175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7076164.1"
                     /translation="GDLRQRLARHHGENVDAMFRGWVEAWQHPTMRDWRMTDRLPAIR
                     CPVLAIQGENDDHGSPGQVEAIVSGVSGPVESWFVPGCGHAPHLEAADAVLERSAAFL
                     RRAFEISDA"
BASE COUNT         1292 a         1975 c         1950 g         1186 t
ORIGIN      
        1 attcgcgcac aggcaagatc acggaggaag gcatgaaggg cgccgctgcg ctgctttcct
       61 tcgacgaaaa gctggacctc agcaaggtcg acctgtcgaa gacgttcgac agccgattca
      121 tcgatgcagc cgcgagcaag aaatagcttt aagagctctc aagtgatcca ggtgcccgcc
      181 atcaatggcg ggcattctgg ttcagcgctc gacaaccggt tgggtacgag gtgtcattca
      241 taccctaggt acgttctgtt acctatgtct ccgggctgga cacttctttg atggcgatcc
      301 cggcaggact cgaacctgcg accctctgct tagaaggcag atgctctatc cggctgagct
      361 acgggacctt tgcctcacat catagccgac gcggggaccc acgtctcgtc ggtggcctca
      421 atgggtccag gcgccgatgc ggccgtatct gaagttgtcg gcgtaggact tgggcaccgg
      481 cctgcgcgga tccggatctt ggaccgtgta ggggatgccg ttgcgctcgg cgtaggcgat
      541 cgcttcttcc ttggtttcga actgcagctg cacctgctcg cgggggtcct gggagcccgt
      601 ccagcccatc agcggatcga tagtccgcgg tcgctgaggt tcgaactcga gcacccagtc
      661 cctggtccgg gcggtgccct gctgcatggc tgaccttgcc ggcttgtaga tccgtgcaac
      721 catatccggt cccactcgtc attcctgctt cgcggccgca acatacgcag tccggggcgg
      781 ccgttcaatg ctgcacatcg gcgccgcagc aaggctaccg cggccgcctt tcccgcgctc
      841 cctgcccggc tcgttagagg gtggttgacc gccagtcgtg ctgctcacta acgtagcagt
      901 cctacccatc cgcatggccg gagacacacg caaatgatcg tggaagaacg cgactatcgc
      961 gtgaaggccg ggaagcttgg ccagttcgtg agcctctacg agcagcacgg cctgccgatc
     1021 cagctcgagc tgctgggcaa gttcatcggt tacttcacga ccgagctcgg ggagctcaac
     1081 catgtcgtgg cgctgtggag ctacgatagc ctcgacgaca ggcagcagcg ccgggaccgc
     1141 atgatggcag acccgcgctg gcaggcgtat ctccagatgg tcgacggtct cctcgacgtg
     1201 cagaccacac gcattctgcg accgacctcg ttctcgcccc tgaagtaatc gtccgaacgg
     1261 agcatcccat gagcacggcg atggtcaacc tccccgacgg cgctgggctc gccgtgacga
     1321 cgagcggcgc agggcgcgat ctcgtgctcg tcaccggcct cggcggaacg gctgcgttct
     1381 gggatcccgt cgtgccgttg ttggagccgc atttccgcgt gacccgcttc gatcagcgcg
     1441 gcatcggcgc cagcgaccgc ggcacggccg aggtgtccat cgggctgctc gctgaggata
     1501 cgcgagccgt gctcgagcac ctggacgcgc gcgatgccgt gctcctcggc cactccaccg
     1561 gcggggtcat cctgcaggaa ctggcgcttc gggattcgtc gcgggtcggc ggcctcgtcc
     1621 tcagcgggac ctgggcgaag cccaaccggt acatgacgga gctgttccgc tcccggtcag
     1681 tgctgctcga gaaggcaccc caggaatacg cagcgatggg tgccttcctc ggctatacac
     1741 cggactggct ggaggccaac tggacgaggt tcgaggccgt cgtcgctgct gcgccaacct
     1801 cacgagctgc gcagaaggtc gtcgcggaaa ggatcgccgc cctgctcgcc ttcgaccgca
     1861 gcaaggacgt tggatccatc agcccgccgg ttctgatcca gggcgccgag gatgacctca
     1921 tcgtcccggc gttcctgcag cgcgaattgg ctgagctgat tccgcaggcc aagctggata
     1981 tccttcccag cggcggccac ttcttcccac tgacgcggcc ggctgaattc ctgaagatcc
     2041 tgacacgttg gaccggcacg ctcgactgag agagcgtgta caagggtttg gagccgcgcg
     2101 gccacgccac gcggctcttt tcacaggaaa agcgccagcc ctatagctgc gggcagatgt
     2161 agcccgtacc agcgggactc ttgaagcagc cgaccgattc cggaagcagc attttcggca
     2221 gccacagcac cacctccgga aagaagatac agaaggtgat cgtgagaaaa aagatgaggt
     2281 aggtcggcag cgaacgcacg acggctgtca tgaagcttac gccaacgaac ttcgaggaca
     2341 tcagaagcga caggccgtac ggtggcgtga tcaggccgaa cgcgagcgtc acgacgatca
     2401 ccacccccat gtgcaccgga ttgacgtttc caagctctgt caatgcggag atgatcggca
     2461 tgaagatgat gattgccggc accgcatcga tgaagtcgcc gatgatgacg aacagggcca
     2521 ccagcaggaa catgatgacc agcggattcg gcccggcaaa gtcggcaatc caccctgcga
     2581 cgacgtccgg cgccttgagg taggctagca tccagccgaa agcggaagcg gcagcaatcg
     2641 cggcgattgg gatcgaataa agcagccctg cattcgcgaa atcgcgtggc aggttgcgga
     2701 tatggccacg ctgcaccagc ggaatcagca cgaacacgat gtaggccaca gcgatgaggc
     2761 cggcctcggt cggcgtgaag aagcccgaca ggatcccgcc catgatgatg atcgggatca
     2821 tcagcggcag agctgaactt ttaaccgcgt cgctgaactg gccgaagctc gcgcgtggtc
     2881 gcaggacccc cacgggtcca acaaaataac tataaatcat gaggccgacg ccgaccatga
     2941 tcccaggagc aatgccgccg agaaagagac cgccgatcga aacattgccg gttgccccat
     3001 agatgacagc catgatgctc ggcgggatca tgttggcgat tgtcgaggcc gcggcgatga
     3061 gggccgcggt gaaggcgcgg tcatagccct cccggtccat ttccggcccg atcgtacgtg
     3121 ccagcacggc aacatcagcg gtcgacgagc ccgaaattcc cgcgaagaac atgctgaaca
     3181 gcgtcacgac ctgggcaagc ccgccgcgaa gatggccgac catcgtctgg gcgagcgtga
     3241 tcaactgcga ggtaatgcgt gcagaggaca taagctcgcc gaccagcagg aaaaatggga
     3301 cggcgagcaa cgcctccgag tcgatgccgt ggaaaagctg gcccactatc gaggagagac
     3361 tgaccggagt gaaactggtc gcgaagaaca cgcccgccat gatggcaaaa gcgaccggta
     3421 cgccgagata gccgaggcca aggaacagcc ccgccatcat gagaatgagg aggccgttag
     3481 gcatcgctgc tctcctggcg agagtcgagc ggcacgggga acccgttctt cagaccattg
     3541 acaatctgct cgacttgaaa caaggcgatg agcgcacccg agatcggcac gctgatggag
     3601 tagtagccga gcggaatgag ggacggcatg cggaaactgc ccatatcgag aaggaagttc
     3661 tgatacccga acacgaccat gcacagtgca actacgagta cgacgagacg cgagaacacc
     3721 tccagatttc gccgcttatt cccgctcatc gtctgaacga tttcggccag atacatgtgc
     3781 tcgttgcggc gagcggcaag cgccatgcca aggaacgagc cgtggatgaa gaaccccgtg
     3841 gtcgcttgtt ggagccagag ccacggatgc ccgatctcgc gcgtgatgac atcacacagc
     3901 actgaaaccg agaagccgac gatgcatacg ccgcacgcga tgaatagaag agcctccagc
     3961 caatcgaggg caggccactt cagatgccgc tcctgcggcg ctaccaaaga tgccattcgt
     4021 ccctgcgtgg atacttcaat ttctcagatg aatgtgcggc agcgcgcagc gttatccgcg
     4081 ctgccctgat gcccgttgga cttaattcac attacgtatc aactcgagga tcttgaccgc
     4141 gtgcggcccg agctcttttg cgagctgatc ctggatcggc ttggccgctg ccatgaagcc
     4201 tgacttgtcc acgtcgcgca ctatcttgac gccgagcttt tcaagcttcg ccgccgaatc
     4261 cttttcgagc tgcaaggact tcgcaggctg cacgcgggcc acctcgtcgg ctgccgcctg
     4321 aacccacttc ttctcgtcgt cgttgaggct attccaggtc ttgtcgctca cccaaatgaa
     4381 gccgttgttt gcctcatgct cggtcaaaga catgatcggc gcgacttcgt aatgcttgtt
     4441 cgacatgtag acgttgatgc cgttctcggc agcttcgacg acaccggttt gcagggaggt
     4501 atagacctca ccgaacggca tatgcacggt ttgtgcgcca taggccggga aatgcgtatc
     4561 ctcggtcttg gtggcctgta cgcgaacctt tacacccttg aggtcttcca ccttcttgaa
     4621 ctcgcgcttg gcgtacatgt tgcgcaagcc gagcgtctcg atacacagaa cgtgcgcacc
     4681 ctgcaccgag tcggaaatca tggtctttac cgcctcgata accttggggt cggcgatggc
     4741 tttcttgaga tggtcttcgt cgcggaaaag gtaatgcagc gacattacgc cagactccgg
     4801 cgccacggtc gcggcattgg ccgatgacgt aataatgaag tcgatgtcgc ccgagcgcag
     4861 cttctgcagc atcacgggct cctgcccgag ctgtgcgccg gggaactggt cgatccccaa
     4921 cttacccccg ctgacctcca tcagcttggc attgaatatg tccgcagcga ggccgtaacc
     4981 agttgatttc ggctgatcat atccaaagct gaacttgcgc tcctgcgcgt agctgtcggt
     5041 cccggcgagg agcgcgatcg ctgcaccggc aatagccgac ccgagaatca aaagcctttt
     5101 tgaaatttgc attcttgtgt cctcccacat agccgccggc aactgaaccg accgcctgct
     5161 ctcttccctt ttggtgctcc gatgatcgaa cttgggcaca attcacgtca agtgttttcg
     5221 ggtaagcgct ttcaattttt tgcagcttgc ggcatgatgt gacgcgacaa gagacagggg
     5281 aaaggacggt cggaaatggc tgcagatccg gtgctgtgct cgatcgagga cggcgttgcg
     5341 ctggtcacgc tcaacaaccc accactcaac ctcgtcacgc tcgagctgac gaggcagttg
     5401 aacagcctgg tcacaaagct cgcggttgat ccctctgtcc gcgtgatggt gctcaccggt
     5461 tccggttcca aggccttctg cgccggatcc gacatcaagg aattccccga catgatggcc
     5521 gctggggctg tcgtgccgaa gaagctggcg ctcgagaacg aggcctacag ccgggtggac
     5581 gacttcccga agccgacgat cgccgcactc aacggcttgg ccttcggcgg tgggctcgag
     5641 cttgcggtgt gctgtgatct ggttgttggc gaagccggta atcaggtcgc gctgccggaa
     5701 atcaagctcg gcgtattccc gggaagtggc ggaacgatcc gcgtgacgcg ccgcatcggc
     5761 gaggcgcgcg cgaaggagat gatgttcttc ggagacccaa taacggccga gactgccctc
     5821 gcttgggggc tgatcaaccg cgtggtgccg aagggcgaag cactcaagac ggcactcgac
     5881 atggcccgca gacttgcggc ccagccgaac gtcgccctgc agatgtgcaa gcagtccgtg
     5941 gacatggcct tcgatacggc agaggaagct gccgtgcgcg cctcgcttgc tctcagcgac
     6001 aaggttttcg cttccgaaga ctgcagcgaa ggcgtccgcg cattttttgc caaggagcag
     6061 ccgaagttca agcatcgctg atctcgaacg cacgccgcag aaatgcggcg ctgcgctcca
     6121 ggaccgcatc ggccgcctcc aaatgagggg catgtccgca gcccggcacg aaccagcttt
     6181 cgaccgggcc gcttacgccg ctgacgatgg cttcgacctg ccccggcgag ccgtggtcgt
     6241 cgttctctcc ctgaattgcg agaacgggac agcggatcgc gggcagccgg tccgtcattc
     6301 gccagtcgcg catggtcggg tgttgccaag cctcgaccca gccgcggaac atggcgtcga
     6361 cgttttcacc gtggtgcctg gccagccgct gccgcaaatc gcc
//
LOCUS       DASVDU010000287         3958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_14772, whole genome shotgun sequence.
ACCESSION   DASVDU010000287 DASVDU010000000
VERSION     DASVDU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14772"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1629
                     /locus_tag="VF226_19180"
     CDS             430..1629
                     /locus_tag="VF226_19180"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7076165.1"
                     /translation="MGYVFKEFTDVDIGMIGQRIPVPYLGAGLPMAYYFKKDVSPQTE
                     KIGFGALAMADLMLVSKSRAGLFRGYIRASFDGRSEYDDDGKLGLSLPKIDDSYYYGA
                     INEAWVQFNGLKVGIQPSLFGFNRVPSILNPTYTSEITTAAASFTIGYAPNFSISVSA
                     EDGGRRNYSEGILSRERSELDKPDWVGLVRYAKGNSLYHFSVARHKATDTVMRDFIGG
                     EATDVSGWAAAAGFQTKIDWSKYLGDAYADNPGRLSLTVAAAEGAMGYLGVPFFAPDY
                     VVSSSGTVERSTGWSAVAAYEHIWAPRWKSTLSYSMFSVNMGSPGEAIIPPEFNMPAP
                     MVDFDITVTGSVLQGGVEHLLSPGLTAGLEVGYTWTKAEGSYAGDKARDLEVSYPHVG
                     IYLRRSF"
     gene            complement(1685..2002)
                     /locus_tag="VF226_19185"
     CDS             complement(1685..2002)
                     /locus_tag="VF226_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076166.1"
                     /translation="MIGANKIIFAMAFGVGAILAAMEPASAKIICRGAFQIVNGQPIA
                     TPYCEDSNVAEVAREYGMHVSAREVRSNPGIKARVCRFIGHDNRVRSACAGYIDSVPR
                     RWF"
     gene            2482..3474
                     /locus_tag="VF226_19190"
     CDS             2482..3474
                     /locus_tag="VF226_19190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HEX7076167.1"
                     /translation="MRHARLPLIGTLLATAALLVSGSPAPAQTTLKWAHVYEVNEPYH
                     TEALWAAEEIKKRTGGKYEIQVFPASQLGNENQINEALGLGTVDIIYTGVAFAGSIHK
                     PIAITNAPFVLRDFNHWKAYRDSPLFRELAKGYEDKTGHKVIALTYYGQRMLTANREI
                     KVPEDMKGMKLRVPPAPLFLMFTKSVGANATPIAFAEVYLALQQGTVDGQENPLPTIM
                     AKKFYEVQSHIMLTGHITESLLTIVGSHVWPKLTDEEKATFNEVLMQAASKATDAIRA
                     SEQKLADEFRKLGKTVVEVDRGPFIAAAQPLHNDPNAGAGWTKEQYDALQALAK"
BASE COUNT          841 a         1208 c         1161 g          748 t
ORIGIN      
        1 gcctacgacg tcgtcgaaac cgatcagggc tatctctcca ccttcacgag cggtgaggtc
       61 agcatcgtca ccctcacctc gcccaacatg actttcgtca gccagttcgg tcgccgccat
      121 tgacgaggtc cgcctcaagc cgccgatccg tcattttcga aaaaagaaat catgatcaga
      181 tataatccga cgatggatca ccggaacctg tttcggtgca aagtccttca tgtcctcgcg
      241 atcggagcgg catctatggc gctattcgct tccgatgcca tcgcccgaga ggctcgcgga
      301 gcggatccgc caacccccag agtggaagag gcaaagaaga ccagtgtaaa gggaccatgc
      361 gccagatacg gtccaaaggc cagggagctg ggaaattcag gaatttgcgc cttcgccggc
      421 ggcgtcgtga tgggttacgt gttcaaggag ttcacggacg tcgatatcgg catgattggg
      481 cagaggatcc ccgtgcccta cctcggtgcg ggtctgccga tggcgtacta cttcaagaag
      541 gacgtcagtc ctcaaacaga aaagatcggc ttcggtgccc ttgccatggc agacttgatg
      601 ctggtgtcga aatccagagc gggtctgttc cgcggataca tcagggcctc tttcgacggc
      661 agaagcgaat atgacgacga cggcaagctg ggcctgagtt tgcccaaaat tgacgattcc
      721 tactactatg gcgccatcaa cgaggcctgg gtacagttca acgggttgaa ggtcggcatt
      781 cagccctcgt tgttcggctt caaccgcgtc ccctccattc tcaatccgac ctacacgtcc
      841 gagatcacca cggccgcggc gtcctttacg atcgggtatg cgccgaactt ctcgatcagc
      901 gtctcggccg aggatggagg ccggcggaac tacagcgagg gcatcctctc acgcgagagg
      961 agcgagctgg ataaaccgga ctgggttgga ctcgtccgct acgccaaagg caattcactc
     1021 tatcatttct cagtcgctag acataaggcc accgatacgg tgatgcggga cttcatcgga
     1081 ggcgaagcga ccgacgtttc cggctgggcg gccgctgccg ggttccagac caagatcgac
     1141 tggagcaagt atctcggcga cgcctacgcc gataatcccg gccggctgag cctcacggta
     1201 gccgccgccg agggagccat gggctactta ggcgtcccct tcttcgctcc ggactatgtg
     1261 gtctcctcgt ccggaaccgt cgagcggtcg accggctggt cagccgtcgc tgcctacgag
     1321 cacatctggg caccgaggtg gaagtcgacg ctgagctatt caatgttcag tgtgaacatg
     1381 ggctcgccgg gcgaagcgat aattccgccg gaattcaaca tgccagcgcc gatggtcgac
     1441 ttcgacatta ccgttaccgg gtccgtcctc cagggtggag tggagcactt gctctctccc
     1501 ggtctcaccg ccggcctcga ggtgggctat acttggacca aggcggaagg aagctatgcc
     1561 ggcgacaagg cgcgcgacct cgaggtcagc tatcctcacg ttggcatcta ccttcggcgc
     1621 tcgttctgag cacgtgcgct ctatgaaagc ctatgcagct tgaacgctcc gcggcggtga
     1681 tgtctcagaa ccaccgcctc ggaaccgaat cgatataacc ggcacacgcg gaccgaacgc
     1741 ggttgtcgtg tccaatgaac cggcataccc gtgccttgat cccgggattg gagcgcacct
     1801 cgcgtgcact gacgtgcatt ccatactcgc gggccacttc cgcaacattg gaatcctcgc
     1861 agtagggcgt tgcgatcggc tggccgttga cgatctgaaa cgcgcctcgg cagatgatct
     1921 tggcacttgc gggctccata gcggccagaa tcgcgccaac tccgaaggcc atcgcaaaaa
     1981 ttatcttgtt cgccccaatc attttctgaa ctccctgcga ccgtaccttc tcgcctacgg
     2041 aacaatagca cagttctggc tcttgcgtga acgcgatcga tcgaaacgag cgaggctgat
     2101 gaattaggtc gtcctcgtca tctggccaga tgtgtttcaa caggcctgaa gcgcagaagc
     2161 tcgcaattgg gaggggagtt gcgctgcacg caacctccca ctcacatcga aaagcaacgt
     2221 ctcgctccgg cgtgctcggc tgggaatgct acgccggcat gctgccaagg ctgctaaggc
     2281 atttacgtgt ccccgctcat cggttcgggc agccactcga cttccgtagg acttgccgct
     2341 gcagcaaagt catcgtatga ctgacaaccg ataacctgat aagtcgccgt gcccgctcgg
     2401 gcgaattccg tggccgtcag gttcatccgt cgaaagagcc ggacaacaag agccggcacg
     2461 aaaccgggag gaaagcacac gatgagacat gcccgactgc cgctgatcgg caccctactc
     2521 gcgactgctg ccctattggt ctccggctcg cccgcgccgg cgcaaacaac gctcaagtgg
     2581 gcgcacgtct acgaggtgaa cgagccctat cacaccgagg ccctgtgggc ggccgaggag
     2641 atcaagaagc gcacgggcgg caagtacgag atccaggtgt tccccgcctc gcagctcggc
     2701 aacgagaatc agatcaacga ggcgctgggc ctcggcaccg tcgacatcat ctacacgggc
     2761 gtcgctttcg ccgggtcgat ccacaagccg atcgccatca ccaacgcacc gttcgtgctc
     2821 cgcgatttca atcactggaa agcctatcgc gactcgccgc tcttccgtga gctggcgaag
     2881 ggctacgagg acaagaccgg gcacaaggtg atcgcgctca cctattacgg gcagcggatg
     2941 ctgacggcta accgcgagat caaggttccg gaggacatga agggcatgaa gctgcgcgtg
     3001 ccgccggcgc cgctgttcct gatgttcacg aagtccgtcg gcgccaacgc cacgccgatc
     3061 gcctttgccg aggtctatct cgccctgcag caggggaccg tcgacggcca ggaaaacccg
     3121 ctgccgacca tcatggcgaa gaagttctac gaggtgcaaa gccatatcat gctgaccggc
     3181 cacatcaccg agtcgctgct gacgatcgtc ggcagccacg tgtggccgaa gctcaccgat
     3241 gaggagaagg ccacgttcaa cgaggtcctg atgcaggccg ccagcaaggc tacggacgcc
     3301 atccgcgcct ctgagcagaa gctggcggac gagttccgca agctgggcaa gacggtggtc
     3361 gaggtcgatc gcgggccgtt catcgccgcg gcgcagcccc tgcacaacga tccgaacgcc
     3421 ggcgcgggct ggacgaaaga gcagtacgac gcgctccagg ctttggcgaa gtaagtatcg
     3481 agaatctccg agccagatgc ccggcggccg tggtgtcgcc gggcgtgacg tacgcagctc
     3541 gaggctttcc gcccttccca ccgaacgatt gcgtttgggc gcgcgctcaa acggtttgtt
     3601 tccatccaaa tcgcaaatgc gctcccggtc agacaaaaac gttcgggatg agtttcttac
     3661 ttttcccgtg aggagaaatg cgaaaaatta aaaccattca tccgcacacg caggagcaag
     3721 ttgtaaggtg gatcccggca actagcgccg ggatgactac ggaaatcgtt tgattggtgt
     3781 ggagcactcg ctatctttca gatgccataa aacaaaaagg gaggaacgtc gtgcagcagc
     3841 aggggcaccc tgaggagcac ctgatccacg tcgaggatgc cgaggtaacg atcgagcatc
     3901 acgccgagga ttgggtatca ttcgcgctat tctgggcgct cgcgctcatt gtcttcct
//
LOCUS       DASVDU010000288        23477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_453528, whole genome shotgun sequence.
ACCESSION   DASVDU010000288 DASVDU010000000
VERSION     DASVDU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 23477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23477
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453528"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1432)
                     /locus_tag="VF226_19195"
     CDS             complement(200..1432)
                     /locus_tag="VF226_19195"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX7076168.1"
                     /translation="MAAKTGDKVKKVVLAYSGGLDTSIILKWLQETYGCEVVTFTADL
                     GQGEELEPARQKAELLGIKPENIFIEDLREEFVRDYVFPMFRANALYEGVYLLGTSIA
                     RPLIAKKQIEIARKTGADAVCHGATGKGNDQVRFELSYYALEPNIRIVAPWREWTFKG
                     RNELLDFARKHQIPIAKDKEGEAPFSVDANLLHSSSEGKVLEDPGVEPPSIVYQRTIS
                     PEDAPDKPTVISIGFERGDAVSLNGERLSPATLLGRLNDLGRDNGIGRVDLVENRFVG
                     MKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDELMPRYAELIYNGFWFAPEREM
                     LQALIDKSQEHVEGEVRLKLYKGNVIVIGRTSPKSLYSSTLVTFEDDRGAYDQKDAEG
                     FIRLNALRLRTLAMRNRG"
     gene            1591..2229
                     /locus_tag="VF226_19200"
     CDS             1591..2229
                     /locus_tag="VF226_19200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX7076169.1"
                     /translation="MPRLEFWYEFASSYSYLSVMRISDLAREAGVEVVWRPFLLGPIF
                     KAQGWETSPFNLYPAKGRYMVRDMQRLAADRGLPFVMPPTFPANSLKAARLALIGADE
                     GWVEPFTRKVFEAQFGRGEEISDVGVISAILAGLGLDASEMLGRAEDPIVKQRLKEQT
                     AEAASRGLFGAPSFVTEAGELFWGDDRLEQALDWVLRPRTGVLPKGNKISGG"
     gene            2277..2525
                     /locus_tag="VF226_19205"
     CDS             2277..2525
                     /locus_tag="VF226_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076170.1"
                     /translation="MRMVSWATALGIAVLAAGFAALGAEAESAKASPCKGLEQKACAA
                     KADCSWVPATTRKDGRKVKAYCRLKAKSSTSAKNAPKT"
     gene            complement(2562..3236)
                     /locus_tag="VF226_19210"
     CDS             complement(2562..3236)
                     /locus_tag="VF226_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076171.1"
                     /translation="MFNLDDQLEWAKIWGDMVLSCMSVATQCTTTMLAPWQQIDALFS
                     SAKPGEPSSSPGQALASWSNMLAPSAWHRPPERSPFEPSWLGLAWVLPLAVPGLALAP
                     QSAIGPLAPMQAWTKLLEASAPMAALGLPTHLLPAGPASFLRSLGWPMAGSPFAIGAA
                     VSIFGPAPARPASVEAPQFAAYRSDSGHAVAQITFPNKVVAAVAVPESAASMIDAIFP
                     WQRLLH"
     gene            complement(3298..3756)
                     /locus_tag="VF226_19215"
     CDS             complement(3298..3756)
                     /locus_tag="VF226_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076172.1"
                     /translation="MLAALLRCFAGYLVACFTAGLVQVFFVLPPHELVFAGADLLSAA
                     GIWLLLATVHSAIFGAPFSIIALAVAEWRRLKSPIYYAAAGFGIAMLGFLTQITGHGF
                     TQPLPVTLYLLAAFTCTGVAAGTAYWLVAGRLAGRRGRRKLEHELDAATQ"
     gene            complement(3794..4255)
                     /locus_tag="VF226_19220"
     CDS             complement(3794..4255)
                     /locus_tag="VF226_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076173.1"
                     /translation="MRYARPQRLEAMFWSTLWRIIVVPVAFLISALIAGLVLVTLGME
                     RITHAFHGQHMDDPDSMVAIFDLVTEGAILASGLTILPALLIVLIGEVARIRSSLYYI
                     IGGGLALVAVPMLAQFGSAGLVLPSPVVWQVFATAGFAGGFVYWLLAGRRA"
     gene            complement(4227..5429)
                     /gene="rlmN"
                     /locus_tag="VF226_19225"
     CDS             complement(4227..5429)
                     /gene="rlmN"
                     /locus_tag="VF226_19225"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7076174.1"
                     /translation="MVLLLDKTPHASPSAAQAESRPSLVGLGRDELREALAAIGVPPN
                     HLRMRVSQLWSWIYVRGVTSFDQMTDVSKDLRARLAAAYSLARPRIVTEQVSVDGTRK
                     WLLRLPKRGREAQAPEIETVYIPETDRGTLCISSQVGCTLNCSFCHTGTQRLVRNLEA
                     QEIVGQIMLARDRIGDWPGAARPNDGRLLPSTERKITNIVLMGMGEPLYNFDNVKAAM
                     DIAADGEGLSISKRRITLSTSGVVPEIPRWGAESGTMLAISLHATRDDLRDELVPINR
                     KYPIAELMAACRAYPGLSNAKRITFEYVMLKGVNDSLADAKALVKLLQGIPAKINLIP
                     FNPWPGTRYECSDWEQIERFAELVNRAGYASPVRTPRGRDIFAACGQLKSETTRLRAS
                     ALRPAAEA"
     gene            complement(5682..6377)
                     /locus_tag="VF226_19230"
     CDS             complement(5682..6377)
                     /locus_tag="VF226_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEX7076175.1"
                     /translation="MKRRTAARLSTQVSMLAVLVNACLLVAIGASHVRAEPGPIRIVA
                     FGDSLTAGYGLNPGEAFPAQLEKALRDKGYAVEVINAGVSGDTTAAGLQRFDWAFPED
                     ANAAIVELGANDALRGIDPAEARQNLDEILTRLEARGIPVLIAGMKAPRNWGDDYVRK
                     FDAIFPELARKHDALLYPFFLEPVAMRPDLNLDDGMHPNGRGVAEIVKDILPEVEKLI
                     ARVDAKRETAPAN"
     gene            6463..7167
                     /locus_tag="VF226_19235"
     CDS             6463..7167
                     /locus_tag="VF226_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076176.1"
                     /translation="MPATAAPIISLDDVRLRLVSRAGPVDILRGVSLEIGAGEAVAIV
                     GPSGSGKTSLLMIIAGLERATSGSVRVAGHEFTELGEDALALIRGREIGIVFQSFHLV
                     PTMTALENVALPLEFAGNPDAFRIAEEMLREVGLGHRTSHFPAQLSGGEQQRVAIARA
                     IAPRPRLILADEPTGNLDGNTGNQIIELLFDLHRRRDATLVLITHDPALAERCARILR
                     MADGRVVADSAKIEAV"
     gene            7164..9746
                     /locus_tag="VF226_19240"
     CDS             7164..9746
                     /locus_tag="VF226_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7076177.1"
                     /translation="MTSAETAVAANIRPGRPAVPQMVRLAVRELRSGFSGFHVFIACV
                     ALGVMVITAVGALTDSLRRGFEQQGELLLGGDVVLSRAHRPADPTERTWINTQGRAAE
                     MATMRTIVRRTDGQDQFLVELKGVDAAYPLVGAVQMQGGADFSDAVHKNGGAVVAPIL
                     LERLGLKVGDSLSLGTITLPITGVVEDEPDSITDNLTYGPRVFVSLETLQRTGLVQLG
                     SLVRWRYALKLTDGRASDERLARFRAQLASALPETGFTVRDRRDPSPRVTRSLDRLRQ
                     FLTLLGLTALLVGGVGVANAVAAFIDRRRKVIATFKSLGATSGMIFGVFLTQVLLIAA
                     IGVVIGLVLGYLVPVIANALYGDALPIRAAVTISPWSIISAAAYGFLVALVFTLWPLG
                     RAELVRAGVLFRDEVAPEPVRPRPRVIALTAATIAALVLFVVVSSEVKWIAISFCLGL
                     VAVFALFAGLGALVTAAARRVPRSRFPEVALAIGNLGAPGGLTRSIVLSLGAGLSLLV
                     AVALTDVSMVRELTTRLPETSPTYFVLDIRKDQAADFRQLVDRVAPGSEVRDAPMLRG
                     RIVELGGRPVDQIKPPPEAEWVLTGDRGLTYSEDVPEGSRVVAGEWWPADYDGEPLVS
                     FEAELAGHLGIGLGDTVTVNVLGRNITARIASLREVKWESLAINFVMVFSPNALRAAP
                     HNLLATITLPRDASLSEDAALARSISNTFPSVTPIRVKDALDAFSDLFEKVMTAVRVA
                     GSITLIAGALVLAGALATAQRRRIKQAVILKTLGAIRRRILVSHFVEYAVLAAVTAGV
                     AAVLGTVSAWIVATRVMEIEFTFSSTAVASALALALILVMGFGGLGTWQVLRAPAVPY
                     LRTD"
     gene            9923..10702
                     /locus_tag="VF226_19245"
     CDS             9923..10702
                     /locus_tag="VF226_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX7076178.1"
                     /translation="MAQYDNRYAQAGTAARTGVAVDEGLRAYMLSVYNYMAAGVALTG
                     LAAYLVFSAAVTGDSGSAVARLGNGTMLTEFGRMMYVSPLRWVIMLAPLGFVFFLSMR
                     VYQMSVSAAQITFWLFAAVMGASLSTIFLVYTGASITRVFFITAAAFGALSLWGYTTR
                     QDISGWGTFLFMGLIGIIIAALVNLFLASSALQFAISVIGVLVFAGLTAYDTQRIKDG
                     YFAVAGNAELAAKTAVMGALSLYLDFINMFTSLLYLFGERE"
     gene            10841..11395
                     /locus_tag="VF226_19250"
     CDS             10841..11395
                     /locus_tag="VF226_19250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7076179.1"
                     /translation="MADIELRSATPDDIPAITAIYRPAVLFGTASFELEPPTEDEMRR
                     RFEAITGNGYPYLVAEISDAVVGYGYANAFRPRPAYRSTVENSIYVAPDHQGKGVGRA
                     LLARLIEVCTEQGFRQMIAIIGDSGQRASIGLHRSLGFIFAGTAHSVGFKHGRWLDTV
                     MMQRALGEGDSTPPRDVRSRATRE"
     gene            complement(11446..11838)
                     /locus_tag="VF226_19255"
     CDS             complement(11446..11838)
                     /locus_tag="VF226_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX7076180.1"
                     /translation="MSDSEPIVFRPRQAGSSSPGPLQSQQGGLAPATISFDRRELDDI
                     LAVYGRKVAAGEWRDYAIDMGRDKAVFSVFRKASECPLYRIEKNPKLARKQGAYSVIA
                     AGGMVLKRGHDLRRVLHVLESKLRLVEH"
     gene            12384..14270
                     /gene="htpG"
                     /locus_tag="VF226_19260"
     CDS             12384..14270
                     /gene="htpG"
                     /locus_tag="VF226_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX7076181.1"
                     /translation="MMTETTSAESHPFLAEVSRLLHLMVNAVYSDREIFLRELISNAS
                     DACDKLRYEAIAHPESLGADDRLHITIIPDKAAGTLTVADNGIGMSRQELMENLGTIA
                     KSGTRAFLEQMQQAEEGSGLIGQFGVGFYSAFMVAESIEVTSRRASSDEAWVWRSDGV
                     SGFSISPPQSNGALPPRGTSVKLKLKKDAFIFLEPDEIERIVRAYSDHILFPIELKLP
                     GEPARQINTASALWHRAKSEVTDEQYTQAYRSITNTIDEPALTVHYKAEGRQSYAVLL
                     FVPKLRPYDLYDPNRKGRVKLYVRRVFITEDAGLLPAYLRFVRGVVDSEDVPLNISRE
                     MLQKNPLVGQIRTAVTGRLLTELERFASRDAKAYEAFFETFGSVLKEGIYEDPTRRDQ
                     LLKLARFRSTAGSGWRSLADYIASMRPNQNEIYFLAGDDPTRLAASPQLEAARARGVE
                     VLLLTDPIDHFWTSLSLKFEGKPFRSLSQGEVDLTAIPLLDESKAAEKEAAGADIKGL
                     IDALRTALGEAVTDVRASKRLTESAVCLVAPRGGPDLGLDRLLQRQQTGVTSRPVLEI
                     NPNHPLITAVARRVADKGKEEAAEWAHLLFDEARILEGDTPADPASFASRMNQMMLKA
                     LGVQ"
     gene            complement(14382..15143)
                     /locus_tag="VF226_19265"
     CDS             complement(14382..15143)
                     /locus_tag="VF226_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX7076182.1"
                     /translation="MTMTRWLTALLILSLPVLVIGSASAGPAGAQHAVSVLELFTSQG
                     CSSCPTADALLRSYASRPDIVALSLPVDYWDYLGWKDTLASPKFTHRQRAYAEERGDG
                     RVYTPQVVVNGLVHVVGSRKDEIDRAIAETSQQLASHRIELTARTDGNTINIEVGAMP
                     AGSDMKGGTIWLAVVQPSVEVEIRRGENSGKKVTYANVVRDLTAIGMWSEKPTTIDLQ
                     QSAVLQEEGQRCAVLLQEGTVGPMVGASWVVGPMH"
     gene            complement(15271..17988)
                     /gene="acnA"
                     /locus_tag="VF226_19270"
     CDS             complement(15271..17988)
                     /gene="acnA"
                     /locus_tag="VF226_19270"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX7076183.1"
                     /translation="MTAQESVSLDSFKSRKTLSVEGKTYSYFSLPDAEKNGLAGISRL
                     PYAMKVLLENLLRHENGRSVSKADISAISAWLENRGKVEKEIGFRPARVLMQDFTGVP
                     AVVDLAAMRDGMNTLGGDPRKINPLVPVDLVIDHSVIVDEFGSPRAFARNVELEYERN
                     LERYEFLKWGQGAFANFRVVPPDTGICHQVNLEYLAQTVWTREIGGETYAFPDTLVGT
                     DSHTTMVNGLAVLGWGVGGIEAEAAMLGQALSMLIPEVVGFKLTGRLREGVTATDLVL
                     TITQMLRKHGVVGRFVEFYGPGLDHMTIADRATIANMAPEYGATCGFFPVDQATLDYL
                     ALTGRESHRIALVEAYAKAQGLFRTADSPDPVFTSTLELDLGTVEPSMAGPKRPEGRI
                     ALDKVGSGFAAALETEYRKTSGATARYKVPGKDFDLGHGDVVIAAITSCTNTSNPSVL
                     IGAGLLARNAVEKGLTVKPWVKTSLAPGSQVVAAYLEKSGLQSYLDQLGFNVVGFGCT
                     TCIGNSGPLAPEISQTINENGLVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAYAL
                     AGSVTKNLAAEPLGIGKDGQPVYLRDIWPSNQEIQNYIAENISREMFRSRYADVFKGD
                     EHWQKIKANPGLTYSWNSGSTYVQNPPYFQGITKEPQPISDISGARILALLGDKITTD
                     HISPAGSIKKDSPAGKYLIERQVRPENFNQYGTRRGNHEVMMRGTFANIRIKNHMVKG
                     ADGNVREGGYTVHYPSGETMFIYDAAMRYREEGVPLVVFAGVEYGNGSSRDWAAKGTN
                     LLGVKAVIAQSFERIHRSNLVGMGVLPLVLEEGLSWQSLGLKGDETVSIRGLADVKPR
                     AKLTAVITAADGRAQEVPLLCRIDTVDELEYYRHGGILHYVLRNLAAAA"
     gene            18172..18828
                     /gene="ccmA"
                     /locus_tag="VF226_19275"
     CDS             18172..18828
                     /gene="ccmA"
                     /locus_tag="VF226_19275"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX7076184.1"
                     /translation="MTDENTIAPDRASRPRLTVEGLAVERGGRRVLTELSFSVEAGEA
                     LLLTGPNGAGKTTLLKTIAGFIRPLSGRVAYSGAAEDRPLAERAHYVGHLNGLRATLT
                     VEENARFWAEYLGGSTGHVLAALERFGLASLSAIPAGYLSAGQKRRLALARLLFAPRP
                     LWLLDEPSSSLDARSTAVLASVLDEHVEAGGIVVAATHLPLGLARSRELRLGFAAGPA
                     "
     gene            18825..19493
                     /gene="ccmB"
                     /locus_tag="VF226_19280"
     CDS             18825..19493
                     /gene="ccmB"
                     /locus_tag="VF226_19280"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX7076185.1"
                     /translation="MSAFWTLVKRDLLIAAREGGATGTALGFYLIVIALMPLGLGPDM
                     QLLSRIAPGVLWIGLLLAALLSLGRMFDTDQDEGVLEILATGPLPMELVAAAKALAHW
                     ISSCLPLAILAPVLGLLLNLELAAYPVLVGTVLAGSPAISFLGSIGAALTLRSRRGGL
                     LIALLVLPLYIPTLIFGISAISAVVTPPGSLGSSLLLLSAVTIASLVIGPISAAAALR
                     MQLS"
     gene            19626..20390
                     /locus_tag="VF226_19285"
     CDS             19626..20390
                     /locus_tag="VF226_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX7076186.1"
                     /translation="MQTLTQRLANPTRFMELSRQLLPWISGLAAVVLAYGLYLSFFAS
                     PPDYQQGETVKIMYIHVPAAWLSMMAYLIIAVSSFGLLVFRHPLADVSAKAAAPIGAA
                     FTLLALITGSLWGKPMWGAYWVWDARLTSVLILFFLYIGLIALRSALDEDPALAGKLT
                     AVLGLVGVVILPVIRFSVDWWNTLHQPASVLKLGGPAIHPSILIPLLVMAAGFTLLFV
                     AMHLKAMRNEILRRRVRSLTMAEVERAARLELRAAE"
     gene            20395..20583
                     /gene="ccmD"
                     /locus_tag="VF226_19290"
     CDS             20395..20583
                     /gene="ccmD"
                     /locus_tag="VF226_19290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX7076187.1"
                     /translation="MDLGPHAAFIWASYAVVALVIALLIVWLIADGKQQERRLEELEA
                     RGVRRRSARASGSGGHAA"
     gene            20588..21226
                     /locus_tag="VF226_19295"
     CDS             20588..21226
                     /locus_tag="VF226_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX7076188.1"
                     /translation="MSGATVQQKESEEVAEVARPRRRRFGWVFAPLVIFGALALMFFY
                     ALQTGDPTRLPSALIGKPVPQFSLEPLPGLVDNGQPVPGFGNRELARGDVSVVNFWAS
                     WCVPCAQEHPVLVELVRRTGVDLYGINYKDQTTAARRFLGRYGNPYRAVGVDGNGRTA
                     IEWGVYGMPETFVVDGNGRIVYKHVGPISMEALETKVIPAIEEAGKARPASG"
     gene            complement(21288..22277)
                     /locus_tag="VF226_19300"
     CDS             complement(21288..22277)
                     /locus_tag="VF226_19300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX7076189.1"
                     /translation="MTLTPHRTTNRWSRGPKAGVLPQGLMALGSAARQRMPRFAISPR
                     KPPEKVYGRIGDLEVRLARSWSDVKRAQRLRYRVFYDEMSAQGSFATHMRGRDEDPYD
                     AVCDHLLVVDTSGIKPSRREWGRQRKPHVVGTYRVLRQEIAERKIGFYTQGEYDIAPL
                     IASKPNYRFMELGRSCVLKPYRTKRTVELLWHGLWTYVREHKVDVMIGCASFEGTDPD
                     SHAMALSFLHHHARAPEEWRVRAHDHLFVPMDRLPPDQVDARAALRAMPPLIKGYLRL
                     GAVVGDGAVIDRQFGTTDVLIILPVERIDPRYFEHFGSPDEQGCRVAQERNVA"
     gene            complement(22405..23286)
                     /locus_tag="VF226_19305"
     CDS             complement(22405..23286)
                     /locus_tag="VF226_19305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7076190.1"
                     /translation="MSGQFSVLAAVLRALLLAGGLMFLFGPVLFPDQFGSAYLSYVGA
                     GLLMGLILDAGLRPGTRRTASAAKGTPEQFAPDPEGVEAERLFREELQASEAHTPRAD
                     RAAPAWSGEQMLAPRTFPSEIDGRPGYSSSAAETPQSAEPIGARVLVAEDNPINARLA
                     QCMLQRAGCIPVLVETGRAAIDAVRKSVEGTGPQIDLVLMDVHMPDLDGLTAAREIRL
                     LDQVTGQTETGRRSPPLIAVTANAFAEDRAMCLAAGMDDYLSKPFSWPELHAMLTRWL
                     PRSDDTGSEQGRAEDAA"
BASE COUNT         4008 a         7433 c         7647 g         4389 t
ORIGIN      
        1 gcatatctga aaagtaaagg atcttgtgca gcttgacgtt gccaagttcc tcgggggagc
       61 agttcgcaca gatgtaatga acgacttcga tcaacttgtc gcgatccagc cggtaaacgg
      121 gtgctttcat ctccatcgtc tgactcctcc cgcatagagt ctgtcacgaa cgaatgggga
      181 acgcaatggt atgccgcctc tatccacgat tccgcatcgc cagagttcgc aacctcaacg
      241 catttaggcg gatgaagccc tcggcgtcct tctgatcgta agccccgcgg tcgtcctcga
      301 aggtgacgag ggtcgaggag tagagcgact tcgggctcgt acggccgatg acaatgacgt
      361 tgcccttata aagcttcagc cgcacctcgc cttcgacgtg ctcctggctc ttgtcgatca
      421 gcgcctgcag catctcgcgc tcgggcgcga accagaagcc gttgtagatc agctcggcgt
      481 agcgcggcat cagctcgtcc ttgaggtggg cggcgccgcg gtcgagcgtg atcgactcga
      541 tcgcccggtg ggcggcgagc aggatggtgc caccgggggt ctcgtagacg ccgcgcgatt
      601 tcatgccgac gaagcggttc tcgacgagat cgacacggcc gatgccgttg tcccggccga
      661 gatcgttgag gcggcccagc agcgtggccg gcgacaaccg ctcgccgttg aggctcaccg
      721 catcgccccg ttcgaatccg atgctaatga cagtcggctt gtccggcgcg tcctctggac
      781 tgatcgtacg ctggtaaacg atggagggcg gctcgacgcc ggggtcttcc agaactttgc
      841 cttccgacga ggagtggagc aggttggcat cgacggagaa cggcgcttcg ccctccttgt
      901 ccttggcgat cgggatctgg tgcttgcggg cgaagtccag aagctcgttg cggcctttga
      961 aagtccattc gcgccagggc gccacgatgc gaatattggg ctcgagagcg tagtagctga
     1021 gctcgaaccg aacctggtcg ttgcctttgc cggtggcgcc gtggcagacg gcatccgcgc
     1081 cggtcttccg ggcgatctca atctgctttt tggcgatcaa cggtcgcgcg atggatgtgc
     1141 cgagcaggta gacgccctcg taaagcgcat tggcgcggaa catcgggaag acgtagtcgc
     1201 gaacgaactc ctcccggaga tcctcgatga agatgttctc gggcttgatc ccgagcagct
     1261 cggccttttg ccgggccggc tccagctcct cgccctggcc gaggtcggct gtgaacgtca
     1321 ccacctcgca gccgtaggtc tcctggagcc acttgaggat gatcgacgta tcgagcccgc
     1381 ccgaataggc aagcaccacc ttcttgacct tgtctccggt cttcgcagcc atcgtaagtc
     1441 ctgctcatgt gctgaaattc gcgcgcacta taggccgcgg tttcgtcgcg ggccaagagg
     1501 cgcctgcgca gcgggtgtcc gcatcgttgt ctcagggcgg atcggaagtg ataagctcgc
     1561 aagcggaacc ggctacctga gaggcccacg atgccgcgcc tggaattctg gtacgagttc
     1621 gcttccagct actcctatct ttccgtgatg cgcatttccg atctggcgcg agaggcggga
     1681 gtcgaggtgg tctggcggcc tttcctgctg gggccgatct tcaaggcgca gggctgggag
     1741 acttctccgt tcaatctcta cccggccaaa ggccgctata tggtccgcga catgcagcgg
     1801 ctggccgccg accgcggact gccgttcgtc atgccgccga cgtttcccgc aaacagcctc
     1861 aaggccgccc ggctggcctt gatcggcgcc gacgaaggct gggtcgagcc attcacgcgc
     1921 aaggttttcg aggcccagtt cgggcgtggc gaggagatct ccgacgtcgg ggtgatttcg
     1981 gccatcctcg ccggcctggg tctggacgcc tcagaaatgc tgggtcgggc cgaagacccc
     2041 atcgtcaagc agaggctcaa ggagcagacc gccgaggcgg catcgcgagg cctattcggt
     2101 gcgccgagct tcgttacgga ggctggcgag ctgttctggg gtgatgaccg gctggagcag
     2161 gccctcgatt gggttttacg tccgcgaaca ggagttcttc cgaaagggaa caagatcagc
     2221 ggcggatgat gttgctaaaa tgttcctgcc gcaattttcg tcggggggaa agtatcgtga
     2281 gaatggtgag ttgggctacg gcccttggca tagcagtgct tgccgcaggg tttgccgcat
     2341 tgggcgccga ggctgagagc gcgaaggcat cgccctgcaa aggtctcgag cagaaagcct
     2401 gcgccgcaaa ggcggactgt agttgggtgc cggcgacgac gcgaaaggac ggccggaagg
     2461 tgaaggccta ttgccgcctc aaggcgaagt cctccacgtc tgcgaaaaac gcgccgaaga
     2521 cttgaggctt taccctcgcg cctgctcgcc gtcctattgg gtcagtggag cagccgctgc
     2581 cagggaaaga tcgcgtcgat catcgaggct gcgctttccg gcacggcaac cgcagcgacg
     2641 accttgttcg ggaaggtgat ctgcgccacg gcgtggccgc tatcggagcg ataggccgcg
     2701 aactgcgggg cttcaaccga agcggggcga gccggagcag gaccgaatat cgatacggcc
     2761 gcgccaatcg cgaacggaga gcccgccatc ggccaaccca gtgaccgcaa gaagctggct
     2821 ggcccggcgg gcaacaaatg cgtgggcagg cccaaggccg ccatcggggc agacgcctcg
     2881 agtaatttcg tccaagcctg catcggcgcc agcggaccaa tcgccgattg cggagctagc
     2941 gccaatccgg gcaccgcaag agggaggacc caggcgagcc ccagccacga gggctcgaat
     3001 gggctgcgtt caggcgggcg atgccaggcc gacggagcca gcatgttgga ccagctagcc
     3061 aaggcctgtc cggggctgga ggagggctca cccggcttgg cggaggaaaa cagggcgtcg
     3121 atctgctgcc agggcgcaag catcgtcgtc gtgcactggg tggcaaccga catgcaggag
     3181 agcaccatgt ctccccaaat tttcgcccac tcgagctgat cgtcgagatt gaacatcgct
     3241 ccgtcctccg gaagctaagg agagacgaat accgcctatt ttgcgatgca gcaagcctta
     3301 ttgcgtagca gcgtcaagct cgtgctccag cttccgccgt ccacgccgtc cggcaaggcg
     3361 gccagccacc agccaatacg cggtcccagc ggctacgccg gtacaggtga atgcggcgag
     3421 cagataaagg gttactggca acggttgagt aaacccgtgg cccgtgatct gcgtgaggaa
     3481 gcccagcatc gcgatcccga accccgcagc ggcatagtag atcgggctct tgagccgccg
     3541 ccattccgcg acagcgagtg cgatgatcga aaacggcgcc ccaaagattg cgctgtgaac
     3601 cgtggcgagc agcagccaga tgccggcagc gctcaggagg tctgccccag cgaaaacgag
     3661 ctcgtgagga ggcagaacga agaagacctg gacaagaccg gctgtgaagc aggcgacgag
     3721 atagccggcg aagcatcgca acagggccgc cagcatagtt ctcccccgct ctggcctatc
     3781 gaactctttg tgttcaggcg cgccgtccag ccagcagcca gtagacgaag cccccggcaa
     3841 agcctgccgt cgcgaagacc tgccagacga ccggactcgg caagaccagg cctgccgaac
     3901 cgaactgcgc cagcattggc accgcgacga gcgcgaggcc gccgccgatg atgtaataaa
     3961 gcgatgacct gatgcgcgcg acctcgccga tcaaaacgat cagcaacgct ggcaggatcg
     4021 tgaggccgga agcgaggatg gcgccctctg ttacgaggtc gaatatggcc accatggagt
     4081 ccggatcgtc catgtgctga ccatggaaag catgcgtgat ccgctccatc ccgagcgtga
     4141 cgaggacgag cccggcgatc agggccgaaa tcaggaatgc caccggaacg acgatgatcc
     4201 gccagagcgt cgaccagaac atcgcctcaa gcctctgcgg ccgggcgtag cgcactcgcc
     4261 ctcaatctgg tggtctcgga tttgagctgg ccgcaggcgg cgaaaatgtc gcgtccgcgc
     4321 ggcgtgcgca cggggctcgc gtagcccgcg cggttcacga gctcggcgaa ccgctcgatc
     4381 tgctcccagt ccgagcactc gtacctggtg cccggccagg ggttgaacgg gatcaggttg
     4441 attttggccg gaatgccctg cagcagcttc accagtgcct tggcatcagc cagggagtcg
     4501 ttgacgccct tgagcatcac gtactcgaag gtgatgcgct tggcgttcga gaggcccggg
     4561 taggcgcggc acgcggccat gagctcggcg atcgggtact tgcggttgat gggcaccagc
     4621 tcgtcccgca gatcgtcgcg tgtggcgtgc agcgagatgg cgagcatcgt cccgctctca
     4681 gcgccccacc tcgggatctc cggcaccacg cccgaggtcg acagcgtgat gcgccgtttg
     4741 gagatggaga gcccctcgcc gtcggcggcg atgtccatgg ccgctttcac gttgtcgaaa
     4801 ttgtagagcg gctcgcccat gcccatcagg acgatgttgg tgatcttgcg ttctgtgctg
     4861 ggcagcaggc ggccgtcatt gggtctcgcc gcgccgggcc agtcgccgat gcggtcgcgc
     4921 gccagcatga tctggcccac gatctcctgc gcttcgagat tgcgcaccag ccgctgcgtg
     4981 cccgtgtggc agaaggagca gttgagcgta cagccgacct gactagagat acagagcgtg
     5041 ccgcggtcgg tttcaggaat gtagaccgtc tcgatttccg gggcctgcgc ctcacgcccc
     5101 cgcttgggca gccgcagcag ccacttgcgg gtgccgtcaa ccgagacctg ctcggtgacg
     5161 atccggggcc gcgcgagaga ataggcggcc gcgagccgcg cccggagatc cttcgagacg
     5221 tccgtcatct ggtcgaagga ggtgacaccg cggacgtaga tccagctcca gagctgcgaa
     5281 acgcgcatgc ggagatggtt cggcgggacg ccgatcgcgg ccagcgcttc ccgcaattca
     5341 tcgcgtccca ggccgacgag cgacggccga ctctccgcct gtgcagcgga cggactcgcg
     5401 tgcggtgtct tatcgagcaa aaggaccatt catcaacctg cgcggggact tgagagcaga
     5461 catttagtcc gtatcgccca cgaggcaaag cccgccgtcg tgcggcccgc ggtcatggca
     5521 gctcgcgtcg gcgagtgtgt cttcacgcca acgccgcacc accgccatac cggggcttat
     5581 cccggtaccc agccatcccc aagcgttgag cccagcagaa cgctggatcc cggcgacgag
     5641 cgccgggatg acgtttagga gttgggctta ggtgcggagc gctaattcgc aggagctgtt
     5701 tctcgcttag catcaacccg cgcgatcagc ttctcgacct cgggcaggat atccttgacg
     5761 atttcggcga cgcccctgcc gttcggatgc atgccgtcgt ccaggttgag atcaggccgc
     5821 atcgcaacgg gctcgaggaa aaacggatag agcagcgcgt catgctttcg agcgagctcc
     5881 ggaaatatcg catcgaactt tcggacgtaa tcgtcgcccc aattgcgcgg cgccttcata
     5941 ccggcgatca gaacgggaat gccacgagct tcgagacggg tgagaatctc atccaggttc
     6001 tgccgtgcct cggcagggtc tatcccgcgg agtgcgtcat tcgcgccaag ctcgacgatc
     6061 gccgcgttgg catcctccgg aaaagcccag tcgaagcgtt gcaggccagc cgcggtggtg
     6121 tcgccggaga caccggcgtt gatcacctcg acggcatatc ccttgtcgcg caaggccttt
     6181 tcgagctgtg ctggaaatgc ttcgccggga ttaagtccat agccggccgt caggctgtcc
     6241 ccgaaggcaa cgatgcgaat gggacccggc tcagcccgga cgtggctcgc cccgatcgcc
     6301 acaagcaggc aggcattgac gaggacagcg agcatgctca cctgtgtcga taggcgggcc
     6361 gcagtccggc gcttcatgat atcgcctcct gtcgcgttca ctgtccaaac caacccccaa
     6421 gtagtagcac gatcgagaga cacaagcttc cggaggccct tcgtgcctgc aaccgcagcg
     6481 cccatcattt ctcttgacga cgtgagattg aggctcgtca gccgcgccgg gccggttgat
     6541 atcctgcgcg gtgtcagtct cgagatcggc gcgggtgagg cggtcgccat cgtcgggccc
     6601 tccggttcgg gcaagacctc gctcctgatg atcatcgccg gcctcgagcg tgcgaccagc
     6661 gggtcggtga gggtcgccgg gcatgagttc accgagcttg gcgaggacgc actggcgctg
     6721 atccgggggc gcgagatcgg gatcgtgttc cagtcgttcc acctggttcc gacgatgacg
     6781 gcgctggaga atgtggcgtt gccgctcgag ttcgcgggca acccggatgc tttccggatc
     6841 gcggaggaaa tgctgcggga agtcgggctg gggcatcgca ccagccattt tccagctcag
     6901 ctctccgggg gtgagcagca gcgggtggcg atcgcgcgcg ccattgcccc ccgaccgcgg
     6961 ctcattctgg ccgacgagcc caccggcaat ctcgacggga acacaggcaa tcagatcata
     7021 gagctgctgt tcgacctcca ccggcggcgc gatgcaacgc tggtgctcat cacgcatgat
     7081 ccggctctgg cggagagatg cgcacgcatc ctgcgcatgg ccgacggccg ggtcgtggcc
     7141 gacagcgcaa agatcgaggc cgtatgacca gcgccgagac ggctgtcgcc gcaaatatcc
     7201 ggcccgggcg cccggccgtg ccgcaaatgg tgcggctggc cgtccgagag ctgcgcagcg
     7261 gcttttccgg ctttcatgtc ttcatcgcct gcgtcgctct cggcgtgatg gtgatcacgg
     7321 ccgtcggcgc cctgacggac tcgctgcggc gcggcttcga gcagcagggc gagctgttgc
     7381 tgggtggcga tgtcgtctta tcgcgcgcgc accgcccggc tgaccccacc gagcggacct
     7441 ggatcaacac gcaagggcgc gctgccgaaa tggcaacgat gcggacgatc gtgcggcgca
     7501 cggacggcca ggatcagttc ctggtggagc tgaagggtgt cgatgcggcc tatccgctgg
     7561 tcggggcggt gcagatgcag ggtggcgccg acttctccga cgctgtccat aagaacggcg
     7621 gtgccgtcgt cgccccgatt cttctggagc ggctgggcct caaggtcggg gacagcttgt
     7681 ccctcggcac catcacattg ccgatcaccg gggtcgtcga agacgagccg gacagcatca
     7741 ccgacaatct gacctacggc ccgcgggttt tcgtgtcgct cgaaacattg cagcggaccg
     7801 gccttgtcca gcttggcagc ctcgtgcgct ggcggtacgc cctgaagctg accgacggcc
     7861 gcgcgagcga tgagcggctg gcgcgatttc gcgctcagct cgcctcggcc cttcccgaga
     7921 ccggcttcac cgtgcgggac cggcgcgatc cgtcgccgcg cgtcacacgc tccctcgatc
     7981 gcctgcgcca attcctgacc ctgctcgggc tgacggcgtt gctggttggc ggcgtaggcg
     8041 tcgcgaatgc cgtcgcggcc ttcatcgacc gccggcgcaa ggtcatcgcg accttcaaga
     8101 gcctcggggc gaccagcggc atgatcttcg gcgtcttcct gacccaggtg ctgctgattg
     8161 ccgccatcgg ggtcgtcatc ggccttgtgc tgggctatct cgtcccggtg atcgccaatg
     8221 cgctctacgg cgatgctctg ccgatccgcg ccgcggtgac gatcagcccg tggagcatca
     8281 tctcggcggc cgcgtacggc ttcctcgtgg ctctggtctt taccctctgg ccgctcggac
     8341 gggcggagct ggttcgcgcg ggcgtgctgt tccgggatga agtggctccc gaaccggtgc
     8401 ggccccggcc acgcgtgatt gccctcacgg ctgcgacgat cgccgcgctc gtgctgttcg
     8461 tggtggtgtc gtcagaggtc aagtggatcg cgatcagctt ctgcctgggg ctggtggcgg
     8521 tattcgcact gttcgccgga ctgggcgctc tcgtgacggc agcagcgcgg agggtgccgc
     8581 gctcgcgctt cccggaagtc gcactggcga tcggtaatct gggggcgccc ggtggcctga
     8641 cgcgatccat cgtgctttcg ctcggtgcgg ggctgtcgct actggtggcc gtcgcactga
     8701 cggacgtctc gatggtacgg gagctgacca cgcgccttcc cgaaacgagc cccacgtact
     8761 tcgtactcga cattcgtaag gaccaggcgg ccgacttccg ccagctcgtc gaccgcgtcg
     8821 cgcccggctc cgaggttcgc gatgcgccga tgctgcgcgg gcggatcgtt gagctcggcg
     8881 gcaggcccgt cgaccagatc aagccgccac ccgaggccga atgggtgctg acgggcgatc
     8941 gcgggctgac ttattcggaa gatgtgccgg agggctcacg cgtggtcgcg ggagagtggt
     9001 ggcctgccga ctatgatggc gagccgctcg tctcgttcga ggccgaattg gccggacatc
     9061 taggcatcgg cctcggagat accgtcaccg tcaacgtgct cggccggaac atcactgcac
     9121 ggatcgccag cctgcgcgag gtgaaatggg agtcgctcgc catcaacttc gtgatggtgt
     9181 tctcgccaaa cgcgttgagg gcagctccac acaacctgct ggcgacgatc accctgccgc
     9241 gggatgcgag cctttccgag gacgcggcgc tcgcgcggtc gatttcgaac acgttcccgt
     9301 ccgtgacgcc catccgcgtg aaggacgcgc tcgacgcatt cagcgacctg ttcgagaagg
     9361 tgatgacagc cgtccgggtg gccggaagca ttacgctcat tgcgggcgct ctcgtgctcg
     9421 ccggggccct ggcaaccgct cagcgtcggc gcatcaagca ggccgtcatt ctgaagactt
     9481 tgggcgccat taggcgccgg attctcgtct ctcactttgt ggaatacgcc gttctggccg
     9541 cggtcacggc cggggtggcc gcggtgctcg ggacggtgtc cgcatggatc gtcgcgactc
     9601 gcgtgatgga aatcgagttc acattttcgt caacagccgt cgccagcgcc ctggcgcttg
     9661 cgctgatcct ggtcatggga tttggcggat tgggcacatg gcaagttctg agggcgccgg
     9721 ctgtgccgta tttgcgaacc gattaagggt taatgacgca cggctgaagt cgaagcccgt
     9781 gatcttgcct cctcgggccc gacttcataa gttctgtcat tgagatatga tcggcgctcc
     9841 gcgccttgaa agcagtgcaa gcgcgggtca tattgcaaac cactggtttt gggtgctcgc
     9901 agcagcggtc aagggatcga aaatggctca atacgacaac aggtacgcgc aagctggcac
     9961 cgccgcccga acgggcgtag cggtcgatga gggcctccgc gcgtacatgc tatcggtata
    10021 caactacatg gcggcgggcg tggccctcac tggtcttgcc gcctacctgg tgttcagcgc
    10081 tgcggtcacg ggcgattctg gctctgccgt cgcccgtctg ggcaatggca ccatgctcac
    10141 cgagttcggc cggatgatgt atgtgagccc gctccgctgg gtgatcatgc tggcgccgct
    10201 cggcttcgtg ttcttcctgt cgatgcgcgt ctaccagatg agcgtcagcg cggcgcagat
    10261 taccttctgg ctgtttgccg cagtgatggg tgcgtctctg tcgacgattt tcctcgtcta
    10321 tacgggcgct tcgatcacgc gcgtgttctt catcaccgcg gcggcgtttg gcgcgctgag
    10381 cctgtggggc tacacgaccc ggcaggatat ctcgggctgg ggcacgttcc tgttcatggg
    10441 cttgatcggc atcatcatcg cggcgctggt caacctgttc ctggcctcga gtgcgcttca
    10501 gttcgccatc tccgtcattg gcgtcctggt gttcgctggc ctcaccgcct atgacacgca
    10561 gcggatcaag gacggctatt tcgcggtcgc tggcaacgcg gagctcgccg caaagacggc
    10621 ggtcatgggg gcgctgagcc tctatctcga cttcatcaac atgttcacct cgctgctgta
    10681 cctgttcggc gagcgcgagt aaggaactga tggccgatct ggccacgtgg caagggcccc
    10741 gctcagtcgg ggcccttttc gttcggggat atcgcgttct gcttttctgt caaaagctgc
    10801 caagctaaaa tgacgcggtc agctgtttga ggcctcgtcc atggcggaca tcgaactaag
    10861 atcagcgacc cccgacgata ttccggccat aacggccatc taccgccccg ccgttctttt
    10921 cggcactgcc agcttcgagc tggagccacc caccgaagac gagatgcgcc ggcgcttcga
    10981 ggcgatcacc ggcaacgggt atccctactt ggtcgccgaa atcagcgatg cagtggtcgg
    11041 ctacggctac gccaacgcct tccggccgcg gcccgcctat cgctccaccg tcgagaattc
    11101 gatctacgtg gcgccggatc atcagggcaa aggcgtggga agagcgctgc tcgccagatt
    11161 gatcgaggtc tgcacggagc aaggcttccg ccagatgatc gcgatcatcg gtgattccgg
    11221 gcagcgtgca tcgatcggat tgcaccgttc gctcgggttc atattcgctg gcacggcgca
    11281 ctcggtcggc tttaagcacg ggcgctggct cgatacggtg atgatgcagc gggcgctcgg
    11341 ggagggtgac agcacgcctc cacgcgacgt gcgcagccgg gccacgcgcg agtagaaccg
    11401 catttgctcg ccccggggct gggataatgc tcgcccgagc tgccttcagt gctccaccag
    11461 ccgcagcttg ctttccagca cgtgcagaac acgccgcagg tcgtggccgc gcttcagcac
    11521 catgcctccg gccgcgatga cgctataggc gccctgcttt ctggcgagct taggattctt
    11581 ctcgattcgg tagagcggac actcgctcgc tttccggaaa acggagaaca cggccttgtc
    11641 gcgtcccatg tcgatggcgt aatcacgcca ctccccggcg gcgacttttc gaccgtaaac
    11701 ggcgaggatg tcatcgagct cgcggcgatc aaaagagatc gttgccggag cgagcccccc
    11761 ttgctgggat tgtagtgggc caggagacga gctgcccgcc tggcgcgggc gaaaaactat
    11821 cggctcggaa tcgctcaaca cgcgcctcct tttgtttccc ctcaatgtcg cttggggctc
    11881 cgggaaattc aagcgcgacc ttcccggatc tggatgtaac aaactgcaat gaaaatcacg
    11941 aagtagccac actcctaccc acgggaggcc caattcatga gcatactgtc caatattgcc
    12001 cctggcccgg tccaaacgct cggagctgtt gccccccata gtccccccga gcttccctct
    12061 ggaccgggcc acctttccct ctcgcaagtt ttcctgccct caggtggccg caggaatgcc
    12121 gcggtatcgc cgcgaatcag aaacagcttg aaagcgttgc cccggcccgg tcctcaggct
    12181 tggcttcgga atgtcagccc ccaagctgcc gaagacgcgt acctgaaccg ggccattttc
    12241 gttgtctcga ggcttaagga accgggcagc gtttgggtaa ccgcgacgat gacctacact
    12301 tgcaacaggc catggcgccg ctttagaaag cgtgccgctc aaaaaaatca taacgcgttg
    12361 aagtcgccgc gagatatgcc ttcatgatga cagagacgac gtcagcagaa tcgcatccat
    12421 tcctggccga ggtgagccgt ttgcttcacc tgatggtcaa cgctgtctat tcggatcgcg
    12481 agatcttctt gcgagagctt atctcgaacg catccgatgc ttgcgacaag ctgcggtatg
    12541 aagccatcgc tcatccggaa tcgctcggcg ccgacgaccg actgcacatc acgatcattc
    12601 cggacaaggc cgccggcaca ctgaccgtgg ccgacaacgg catcggcatg agtcgccagg
    12661 agctcatgga gaacctgggg accatcgcca agtccggcac cagggccttc ctcgaacaga
    12721 tgcaacaggc cgaggagggc agcgggctca tcggccaatt cggcgtgggg ttctactcgg
    12781 ccttcatggt ggccgagagc atcgaggtga ccagccgcag ggcgagctcc gacgaagctt
    12841 gggtctggcg atcggacggg gtgtccgggt tttcgatttc gccaccccaa tccaatggcg
    12901 cgctgcctcc tcgcggtacc tcggtaaagc tgaagctcaa gaaggacgcg ttcatcttcc
    12961 tcgagcccga cgagatagag cgcatcgtcc gcgcctactc cgaccacatt ctgtttccga
    13021 tcgagctgaa gctgcctggt gagcccgcac gccagatcaa cacggcgagt gcgctgtggc
    13081 accgggcgaa gtccgaggtg acggacgagc agtacacgca ggcctaccgc tcgatcacca
    13141 acaccatcga tgagccggcg ttgacggttc actacaaggc tgaagggcgg caatcgtatg
    13201 ccgtgctgct tttcgtgcct aagctgcggc cctacgatct ctacgatccg aaccgcaaag
    13261 gccgcgtgaa gctttacgtc cggcgcgtct tcattaccga ggacgccggg cttctcccgg
    13321 cttatctccg gttcgtccgt ggtgtcgtgg acagcgagga cgtccccctc aacatctccc
    13381 gggagatgct gcagaagaac ccgctggtcg gccagatccg gacggcggtg acaggccggc
    13441 tgctcaccga gctggaacgg ttcgcctcgc gcgatgccaa ggcctacgaa gcctttttcg
    13501 agaccttcgg gtccgtgctg aaggagggta tctacgagga tcccacgcgg cgggatcagc
    13561 tcctcaagtt ggcccgcttc cgcagcacgg cggggtcggg atggcggtcc ctcgccgact
    13621 acatcgccag catgcggccg aaccagaacg aaatctactt cctcgcgggc gacgatccga
    13681 cccgtctggc cgccagcccg cagctcgagg ccgcgcgtgc ccgcggagtc gaggtgctct
    13741 tgctcaccga cccgatcgac cacttctgga cgtctctgag cctgaagttc gagggcaagc
    13801 cgttccgctc attgagccag ggcgaagtcg atctcaccgc catccctctg ctggacgaga
    13861 gcaaggccgc ggagaaagag gcggcgggcg cagacatcaa agggcttatc gatgccctgc
    13921 ggacagctct cggcgaggcg gtcaccgacg tgcgtgcttc gaaacgattg accgagagtg
    13981 cagtgtgcct ggttgcgccc cgcggcgggc cggatcttgg tctagacaga ttgcttcagc
    14041 gccagcagac gggcgtgaca tcgcggccgg tgctcgagat caacccgaac catccactca
    14101 tcacagccgt cgcccggcgt gtcgctgaca agggcaagga agaggctgcc gagtgggcgc
    14161 acctgctgtt cgacgaggcg cgcatcctcg aaggcgatac ccctgccgat cccgcgagct
    14221 ttgctagccg catgaaccag atgatgctga aagcgcttgg cgtgcagtga aggggagctg
    14281 ctagcgtcgt agcccctcac ccggccctcc gggccgacct ctccccatgg gagaggttaa
    14341 gggagtgtct gctgctaact ggggttccag cttagtcgga tttagtgcat gggccccacg
    14401 acccagctcg ccccgaccat gggtccgacg gtgccttcct ggaggagcac ggcgcagcgc
    14461 tgcccttctt cctgaaggac ggccgattgc tgcaggtcga tcgtggtggg tttttccgac
    14521 cacatgccga tagcggtgag gtcgcggacg acgttggcgt aggtgacttt cttgccgctg
    14581 ttctcgccgc gcctgatctc cacctccacg ctgggttgca cgacggccag ccagatcgtg
    14641 ccgcccttca tatcgctccc ggccggcatg gcaccgactt cgatgttgat cgtgtttccg
    14701 tcggttcggg ctgtcagctc gatgcggtgc gatgcgagct gctgggaggt ctcggcgatg
    14761 gcccggtcga tctcgtcctt ccggctgccg acgacgtgaa cgaggccatt cacgacgact
    14821 tgcggggtgt agactcgacc atcaccacgc tcttcggcat aggcccgttg ccggtgagtg
    14881 aacttcgggc tggcgagcgt gtctttccag ccgagataat cccagtagtc gacaggcagc
    14941 gagagcgcga cgatgtccgg tcgcgacgcg tagctgcgca gcagggcgtc ggcagtgggg
    15001 caggacgagc agccctggct cgtgaacagc tcgaggacgg aaacggcatg ctgcgctcct
    15061 gccgggccgg ccgatgccga cccgattacg aggaccggca gggaaagaat gagaagcgct
    15121 gtcagccagc gagtcatagt catccttgag cggtttcgag cgaaccttat ccgcgcgtcg
    15181 gcctccaaat gccagtcacg aatgggtgag ttgcccgctt accttcaagt ggaagtggaa
    15241 aggccggcta aggggccggc ctttcccgga tcaggccgcg gcagcaaggt tgcgcagcac
    15301 gtaatgcagg atgccgccgt ggcgatagta ctccagctca tccacagtat cgatccggca
    15361 gagcagcggg acttcttgcg ccctaccgtc agccgctgtg atgacggcgg tcaacttcgc
    15421 ccgcggcttg acgtccgcga gtccgcggat gctgaccgtc tcgtcgccct tgagcccgag
    15481 tgattgccag ctcagcccct cttctaagac gagcggcagc acgcccatgc cgacgaggtt
    15541 cgaccggtgg atgcgctcga aggattgcgc gatcaccgcc ttcacaccaa ggaggttggt
    15601 gcccttggcc gcccagtcgc gggacgagcc gttgccgtac tcgacgccgg caaacacgac
    15661 cagcggcacg ccctcctcgc ggtagcgcat cgccgcgtcg tagataaaca tcgtctcgcc
    15721 cgaggggtaa tggacggtgt agccaccttc gcggacgttg ccatctgcgc ccttcaccat
    15781 gtggttcttg atgcggatgt tggcgaaggt gccccgcatc atcacctcat gattgccgcg
    15841 ccgtgtgccg tactggttga agttttcagg ccgcacctgc ctctcgatca gatactttcc
    15901 ggccgggctg tccttcttga tcgagccggc gggcgaaatg tggtccgtgg tgatcttgtc
    15961 gcccagcagc gccaggatgc gcgcgccgga aatatcggag atcggctgcg gctctttcgt
    16021 gatgccctgg aagtagggcg ggttctgcac gtaggtggag ccgctgttcc aggagtacgt
    16081 caggcctggg ttggccttga tcttctgcca gtgctcgtcg cctttgaaca cgtccgcgta
    16141 gcgcgacctg aacatctcgc gtgagatgtt ctcggcgatg taattctgga tctcctggtt
    16201 gctcggccag atgtcgcgga ggtagaccgg ctggccgtcc ttgccgatgc cgagcggctc
    16261 ggcggcgaga tttttcgtca ccgatccagc cagcgcgtac gcgacgacga gcggtggcga
    16321 ggcgagatag ttcgcctgca catcggggct gacgcggccc tcgaaattgc ggttgccgga
    16381 gagcacggcc gccgcgacga ggccgttctc gttgatcgtc tgcgagatct cgggcgcgag
    16441 tggccccgag tttccaatgc aggtcgtgca gccaaatccc accacgttga agccgagctg
    16501 gtccagatag gactgcagcc cagacttctc gaggtaagct gcaacgactt gcgagccagg
    16561 cgccagcgag gtcttcaccc acggcttgac cgtcaggccc ttttcgacgg catttcgcgc
    16621 gagcagcccg gcgccgatga gcacgctcgg gttggaggtg ttcgtgcagc tcgtgatcgc
    16681 cgcgatgacg acgtcgccgt ggccaagatc gaaatccttg ccgggcacct tgtagcgtgc
    16741 ggtggcgcca ctggtcttgc ggtactcggt ttcgagcgcg gcagcgaagc cgctccccac
    16801 cttgtcgagc gcgatacgcc cttccggacg ctttgggcct gccatcgacg gctcgaccgt
    16861 gccgaggtcc agctccagtg tggacgtgaa gaccggatca ggagagtcgg ccgtgcggaa
    16921 cagtccctgc gccttggcat aggcttcgac cagcgcaatg cggtgcgact ctcgacccgt
    16981 aagggcgaga tagtcgagcg tcgcctggtc gacggggaag aagccgcacg tcgcaccata
    17041 ctcgggcgcc atgttggcga tcgtcgcccg gtcggcgatg gtcatgtggt cgaggcccgg
    17101 cccgtagaac tcgacaaacc gcccgacgac gccgtgcttg cgcagcatct gcgtgatggt
    17161 cagcacgagg tcggtggcgg tgacgccctc tctaagccgg ccggtcagct tgaagccgac
    17221 gacctccgga atgagcatcg acaacgcctg gccgagcatg gctgcctcgg cctcgatacc
    17281 gcccacgccc cagcccagca ccgcgaggcc gttgaccatc gtggtgtggc tgtcagtgcc
    17341 gacgagcgtg tcagggaagg cgtacgtctc gccgccgatc tcgcgggtcc agaccgtctg
    17401 ggcgagatat tccaggttca cctgatggca gatgccggtg tccggcggaa cgacgcggaa
    17461 gttggcgaac gcgccctggc cccatttcag gaattcgtag cgctcgaggt tccgctcgta
    17521 ttcgagctcg acattgcgag cgaacgcccg ggggctgccg aactcgtcca cgatcaccga
    17581 gtgatcgatg accaggtcca ccggcacgag cgggttgatc ttgcgcggat cgccgccgag
    17641 cgtgttcatg ccatcgcgca tggcggccaa gtccacaacg gccggcacgc cggtgaagtc
    17701 ctgcatcagc acgcgggctg ggcgaaaccc gatttccttc tctaccttgc cgcgattttc
    17761 gagccaggcc gagatagcgc tgatatccgc cttgctcacc gagcggccgt tctcgtgccg
    17821 cagcaggttt tccagcagca ccttcatcgc gtaaggcagg cgggaaatac cggcaaggcc
    17881 gttcttctcg gcatccggca atgagaaata ggagtaggtc ttaccttcga ctgagagtgt
    17941 cttgcggctc ttgaagctgt ccaacgacac ggattcttgc gcggtcacga tttgtcctcc
    18001 atccgagcaa ctgaagccgc gccgccggag cgccggcgga gggatcaacc ccgtttttgc
    18061 gctagcgtcg atagaagcgt gctccttata taaccgcgct cgcgcgaggg ccagctcacg
    18121 gttgtcccac ttggggcgcg caggacgtac gcggtcggaa gacaggttgc ggtgacggac
    18181 gagaatacga ttgcgcctga tcgggcctcc cgtcccaggc tcacggtcga ggggcttgcg
    18241 gtggagcgcg gcgggcggcg cgtgctcacg gagctgtcat tcagcgtgga agccggcgag
    18301 gccctgcttc tgaccggccc caatggcgcg ggcaagacga cactcctgaa gacgatcgcc
    18361 ggcttcatcc ggccgttgtc aggccgcgtc gcgtattcag gcgctgcgga agaccggccg
    18421 ctggcagagc gggcgcatta tgtcggccac ctgaatggcc tgcgcgcgac ccttaccgtc
    18481 gaggagaatg cccggttctg ggcggagtac ctcggcggat cgaccgggca cgtccttgct
    18541 gcgctcgagc ggttcggcct tgcctctctt tcggcgatac cagcgggcta tctctcggca
    18601 gggcagaagc ggcggctggc attggcccgg ctgctgttcg cgccgcgccc gctctggttg
    18661 ctcgacgagc cgtcatcctc cctcgatgcg cggtcgacag cggtgctggc cagtgtcctc
    18721 gacgagcatg tcgaggcggg gggcatcgtg gtcgctgcga cgcatctccc cctcgggctg
    18781 gcccgctcgc gcgagctgcg gctcggcttt gccgcggggc cggcatgagc gccttctgga
    18841 cgctcgtgaa gcgcgacctg ctcattgcag cgcgcgaggg aggcgcgacg ggaacggccc
    18901 tcggcttcta cctgatcgtc atcgccctga tgccgctggg cctcgggccc gacatgcagc
    18961 tcctgtcgcg gatcgcgccg ggcgtgctgt ggatcggcct gctgctggct gcgctactgt
    19021 cgctcggccg catgttcgac acggaccagg acgagggcgt gctggaaatc ctggcgaccg
    19081 gcccgctccc gatggagctg gtggccgcag ccaaggcgct cgcgcactgg atatcgagct
    19141 gtttgccgct cgcgatcctc gcgccggtat tgggattgtt gctcaatctc gagctggccg
    19201 cctatcccgt gctcgtcggg accgtgctcg ctggctcgcc cgccatcagc tttctcggca
    19261 gcatcggtgc ggcgttgacg ctcaggagcc gccgcggcgg cctgttgatc gcgctgctgg
    19321 tgctgcctct ttacattccg accctgattt tcgggatctc cgcgatcagc gccgtcgtaa
    19381 ccccgcctgg cagcctgggg tcatcgctgc ttctgctttc ggcagtcacg attgcgtcgc
    19441 tggtcatagg cccgatttct gccgcggccg cacttagaat gcagctcagt taaacgaccg
    19501 agctcgttgc gctacggctg gtgcaccggc caaggcggcc atgacggacg agcaaatagc
    19561 gttgttcatg ccccgcgacc tctaagcggt ttgcgcgcgg cgcttggacg gcctagatca
    19621 gtgccatgca gacgctcacc caaaggctgg ccaatccgac gcggttcatg gagctgtcac
    19681 gacagctcct gccctggatc tccggccttg cagccgttgt gctggcctac gggctctatc
    19741 tcagcttttt cgcctcgcct cctgactatc agcagggcga gacggtcaag atcatgtaca
    19801 tccatgtccc cgcggcgtgg ctgtcgatga tggcctacct gatcatcgct gtgtcgagct
    19861 tcggcctgct cgtcttccgg catccgctgg ccgacgtctc cgccaaagcg gcggcgccaa
    19921 tcggagcggc ctttaccttg ctggcgctga tcaccgggtc gctgtggggc aaaccgatgt
    19981 ggggcgccta ctgggtctgg gacgcccggc tgacgtcggt gttgatcctc ttcttcctct
    20041 atatcggcct gatcgcactg cgctccgcgc tcgacgagga ccctgccctc gctggcaagc
    20101 tgacggccgt gctggggctg gtcggcgtcg tgattttgcc ggtgatccgc ttttcggtgg
    20161 actggtggaa cacgctgcat cagccggcga gcgtgctcaa gctcggtgga ccggcgattc
    20221 acccttccat tctgatcccg ctcctggtga tggctgccgg ctttaccctg ctgttcgtgg
    20281 ccatgcatct caaggccatg cgtaacgaaa tcctccggcg gcgggtgaga tcgctcacga
    20341 tggccgaggt tgaacgggcg gcccgcctcg aattgcgcgc tgcggagtag cgtcatggat
    20401 cttggacccc acgccgcgtt catctgggcc tcttacgccg tggtggcgct cgtcatcgcg
    20461 ctgcttatcg tctggctcat cgcggacggg aagcaacagg agcgccggct ggaggagctg
    20521 gaagcgcgcg gagttcgccg tcgctcggcg cgcgcatctg gctctggcgg ccacgcggct
    20581 tgaacagatg tcgggcgcaa cggtgcagca gaaggagtca gaagaggtag cagaggtagc
    20641 gcgacccagg aggcgccgct tcggctgggt cttcgcgccg ctcgtcatct ttggcgctct
    20701 cgcgctgatg ttcttctatg cgctgcagac cggtgatccg acacggctgc cgtcggcgct
    20761 gatcggcaag cctgtccctc agttctcgtt ggagccgctc ccagggctcg tcgacaacgg
    20821 ccagccggta cccggtttcg gtaatcgcga gctcgcacgc ggcgatgttt cggtcgtgaa
    20881 cttttgggcg tcctggtgcg tgccgtgcgc gcaggagcat ccggtcctgg tcgagctggt
    20941 ccgccggacc ggggtcgatc tctacgggat caactataag gaccaaacga ccgcggcacg
    21001 ccgcttcctt ggtcgttacg gcaatcctta ccgggctgtc ggcgtcgacg gcaacgggcg
    21061 cacggcgatc gagtggggcg tctacggcat gcccgagaca ttcgtcgtcg atggcaacgg
    21121 ccgcatcgtc tacaagcacg tcggcccgat ttcgatggag gcgctggaga ccaaggtcat
    21181 cccggctatc gaggaagctg gaaaggcgcg ccctgcttcc ggatgagttc cggcggttag
    21241 actagggaga tttccgtggc tgccgtcggc gaggcccggt atgccggtca agcgacattc
    21301 cgctcctgag cgacgcggca gccctgctca tcgggcgatc cgaagtgctc gaagtagcgg
    21361 gggtcgatcc gctccacagg caagatgatc agcacgtccg tcgtgccgaa ctgccggtct
    21421 atgacggccc cgtcgccaac gactgcgccg agccgaagat agcccttgat cagtggcggc
    21481 atggcgcgca gagcggcccg ggcgtcgacc tggtccgggg gtagccggtc catgggcacg
    21541 aacaggtgat catgtgcccg gacacgccat tcttccggcg cgcgtgcatg gtggtgcagg
    21601 aacgacaggg ccatcgcgtg cgagtccggg tccgtgcctt cgaagctggc gcagccaatc
    21661 atcacgtcga ccttgtgctc gcgcacgtag gtccacagtc catgccacag caactcgacg
    21721 gtgcgtttgg tgcggtacgg cttcagcacg cagctccgcc ccagctccat gaagcggtag
    21781 ttgggcttcg aggcaatcag cggagcaatg tcgtactcgc cctgcgtgta gaacccgatc
    21841 ttccgctccg cgatctcctg acgcaggaca cgataggtgc cgacgacatg cggcttgcgc
    21901 tgccggcccc attctcgcct gctcggcttg atgccggagg tgtcgacgac cagaagatgg
    21961 tcgcaaacgg cgtcgtaggg gtcctcgtcg cggccgcgca tatgggtcgc gaagctcccc
    22021 tgagccgaca tctcgtcata gaacacgcga tagcgtagcc gctgcgcgcg tttcacgtcg
    22081 gaccacgagc gggcgagccg cacctcgagg tcgccgatgc ggccataaac cttttcaggt
    22141 ggttttcgcg gggagatagc gaaccgcggc atgcgttgcc gggccgcgga gccaagggcc
    22201 atcaggccct gaggcagaac acccgccttg ggcccgcgcg accagcggtt cgtcgtgcga
    22261 tgcggagtga gcgtcatgca gccttcgcag cccccagttg cctcgggtca cctcggactc
    22321 gggcaaggcg ctccatgccc tcttaaccat tagcataaaa aaacgacgaa tcggaaacaa
    22381 acgcgtgtgg gagcgtcgtg ggaattaggc ggcgtcctcg gcgcggccct gctcagagcc
    22441 ggtatcatcc gatcgcggca gccatcgcgt gagcatcgca tgaagctctg gccaggagaa
    22501 gggcttggac aagtagtcgt ccatccctgc tgcaagacac atcgcgcggt cctccgcaaa
    22561 ggcattggcg gtgacggcga tgagcggcgg tgacctccgg ccggtttcgg tctggccggt
    22621 gacttggtcg agcagccgaa tctcgcgtgc tgcggtaagc ccgtcgaggt ccggcatgtg
    22681 aacatccatc aggaccagat cgatttgcgg gcccgttccc tcgacactct tgcggactgc
    22741 gtcgatggcg gcgcggccgg tctcgaccaa taccgggatg catccggcgc gctgcagcat
    22801 gcactgcgcg agcctggcgt tgatgggatt gtcctcggcg acgaggacgc gcgcgccgat
    22861 gggttcagcc gactgtggcg tttcggccgc gcttgacgag tagcctgggc gcccatcgat
    22921 ctcgctcggg aatgtgcggg gcgcgagcat ctgctcgccg gaccaagcag gggcggctcg
    22981 atctgcacgc ggcgtgtgtg cttcggaagc ctggagctcc tcgcggaaga gccgctctgc
    23041 ttcgacaccc tcggggtccg gcgcgaattg ttctggagtg cctttcgcgg cgctcgcggt
    23101 ccgacgggtg ccggggcgca gacctgcgtc caggatcagc cccatcagca atccggcccc
    23161 gacgtagctc agatacgcgg atccgaactg gtcaggaaac agaacagggc cgaagaggaa
    23221 catcaaacct cccgcgagaa gcaaggcccg caggactgcg gcgagcacgg agaattgccc
    23281 actcatacag atcctcctcg agaccgtcga agcgtgttcg cctgccgccc caagcgaagc
    23341 tgtcggtccg acgacggatg atcgatagcg tcggactgac cgcgcccgct tataaggcaa
    23401 tcagcccggc gttgaggaag ccttcaagaa gctggttaac cgctggtgcc gtgccgcttc
    23461 gttcgcggat cgaccgt
//
LOCUS       DASVDU010000289         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_76095, whole genome shotgun sequence.
ACCESSION   DASVDU010000289 DASVDU010000000
VERSION     DASVDU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76095"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..947
                     /locus_tag="VF226_19310"
     CDS             261..947
                     /locus_tag="VF226_19310"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEX7076191.1"
                     /translation="MASHRTLSVPSKTAYLQAMSGQRPAILLTGFGPFPGVPENASSR
                     LVPELAAEARKRFPDYHVEAAILPTEWRGGPAAASDLLDALNPAVALHFGVSSQARAI
                     TIESRAHNHARPVPDAAGLLPPSEKLSMDGPELLAARFPAYHIAGRLRRRGLPVILSR
                     DAGGYLCNAVLYHSLERAWRADRQMRSGFIHLPKALGEMKVSDETATQLDWDQAIEGG
                     LEIIAATLRR"
     gene            complement(944..1954)
                     /locus_tag="VF226_19315"
     CDS             complement(944..1954)
                     /locus_tag="VF226_19315"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX7076192.1"
                     /translation="MANPALVELLRGGRVESRHRGSVAIAGPDGRLALAIGEVHRPVF
                     PRSAIKAIQCLPLIETGAADHFGFGAAEIALACASHSGTERHVAVAAGMLAKAGLDAG
                     ALACGAHEPLSPTAARRLIQEGRPLSPLHNNCSGKHAGMLATAVHMAEPVADYWRPDH
                     PVQVRIAEALASLTGTPIGKDQLGIDGCSAPNWAIPLANLARAFARIVSGEGLDAKRR
                     DAAARIVQACWAEPELVAGPERLDSVLMTQFRGSLFLKTGAEGVYCGAVPARGLGFAL
                     KIDDGAKRASEMALKALVSRLVPDAHDLAAEETVPNWRGLPAARLRCASAWLQSIADA
                     GF"
     gene            2052..2681
                     /locus_tag="VF226_19320"
     CDS             2052..2681
                     /locus_tag="VF226_19320"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7076193.1"
                     /translation="MYKLISRPGWGSIMAEAQLDWYGLDYTIEDVDDLFKSAEARESV
                     QRLNPLAQLPTLILPSGAVMTESAAITLYLADVTRSNELVPAPDELLRPAFLRWLVFI
                     VANIYPTFTYADDPSRFVPGEEAQKGFRENVDAYARRLWGILESEAGQPWFLGERLSA
                     LDLYISAMSRWRPKREWFAANAPRLHAIGTAFEALPQVQSVWQRNFPAA"
     gene            complement(2926..>3515)
                     /locus_tag="VF226_19325"
     CDS             complement(2926..>3515)
                     /locus_tag="VF226_19325"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7076194.1"
                     /translation="ELQQFRDKWPVGTKPRLAFEILYWTGCRVSDLAQLGRQHVRSNR
                     IVFTAHKNRNRSPVRIDIPAMPELLAVIAKSPCGDLTFLTTDWGPPYTSHKGLSRRFV
                     EWCREAGLPAGCSAHGIRKAAACELALKGANLNQLQAVFGWKTAKEALRYTQAADRAR
                     LSDEAFVLRSKAESRTLCSHFPLTTGEVRTFRPKK"
BASE COUNT          634 a         1174 c         1075 g          632 t
ORIGIN      
        1 gtcgcgcgat cacgcggcac agcggattgc ggagctggag gaggaagtcc gtcacggccg
       61 gatgacggcg ccgcgggcgg tcgacgaagc tcttcgtctc ctcgagaagt gaccagtccg
      121 gcatggactg gccacctctt ggcttggacc agtgggacag gaagccgctc gtttccgcaa
      181 gccaggctgc tggccctgca agccccatgt gcccccggcc gagctgaccg ccacgaccgg
      241 actgttgcta gagaggtgtt ttggctagcc accgcaccct ttccgtaccc agcaaaacgg
      301 cttatctcca agccatgtcc gggcagcgtc cagccattct tcttaccggc ttcggcccct
      361 tccccggggt tccggagaac gcctcgtctc ggctcgttcc cgagctggct gccgaagcgc
      421 ggaagcgctt tcctgattat cacgtcgagg cggccatact ccccaccgag tggcgtggcg
      481 ggccggctgc cgcgtccgat ttgttggatg cgctgaatcc ggccgttgcc ctgcacttcg
      541 gcgtgtcgag ccaggcacgc gccatcacga tcgaatcccg cgcgcacaat catgcgcggc
      601 cggttcccga tgcggcaggc ctgctgccgc cgtctgaaaa gttgtcgatg gatggcccgg
      661 agctgctcgc ggcacgcttt ccggcctatc acatcgccgg acggctccgg cggaggggat
      721 tacctgtcat cctgtcccgc gatgcgggtg gttatctctg taacgccgtg ctttatcatt
      781 cgctcgagcg agcgtggcgg gcggaccggc agatgcggag cggctttatc cacctgccga
      841 aagcgctcgg cgaaatgaag gtttcggatg aaaccgcaac ccagctcgac tgggatcagg
      901 ccatcgaggg cggccttgaa atcattgccg ccacactcag acgctagaag ccggcgtccg
      961 caatcgactg gagccaggcg gaagcacagc gtagccgtgc agcaggaagc ccacgccagt
     1021 tgggaacggt ttcctcagcg gccagatcgt gcgcatcggg cacgaggcgt gaaacaagtg
     1081 ccttgagcgc catttccgag gcgcgcttcg cgccgtcgtc gatcttcagc gcaaatccca
     1141 gaccgcgggc cggaacggcc ccgcagtaga ccccctctgc gccggttttc agaaacaggg
     1201 atccgcgaaa ctgcgtcatc agaaccgaat ccagccgctc gggtccggcc accagctccg
     1261 gttcggccca gcacgcttgc actatcctcg ccgccgcatc gcgccgtttc gcatcgagcc
     1321 cctcgcccga gacgatccgg gcaaaggcgc gcgccaggtt ggccagcggt atggcccagt
     1381 tcggggccga gcaaccatcg atacccagct ggtcctttcc gattggggtg cccgtcaggc
     1441 tggccagcgc ttcggcaatg cgcacctgaa ccggatggtc cggacgccag taatcggcga
     1501 cgggctcggc catgtgcacc gctgtcgcca gcatccctgc gtgcttgccc gaacagttgt
     1561 tgtggagcgg cgacagcggc cggccctcct ggatcagcct gcgtgcagcg gtgggcgaca
     1621 gcggctcgtg cgctccacag gccagcgccc ccgcgtccag ccccgccttc gccagcatcc
     1681 ccgcagccac ggcgacgtgg cgctccgttc cgctatgaga ggcgcaggca agcgcgatct
     1741 cggcggcgcc gaacccgaaa tgatcggcgg cgcccgtctc gatcagcggc aggcactgga
     1801 tcgccttgat ggcagagcgc gggaagacgg gccggtggac ttcgcctatg gcaagcgcca
     1861 aacggccatc cgggccggca atggcgaccg agccgcggtg gcgactttcg acgcgcccgc
     1921 cgcgcaacaa ttccaccaga gcaggatttg ccatctcgcc ctctgccgct cacatcgagg
     1981 gtggagcctt gcgcatcgcc cctcgtttcc tgcctattga cgatccgtcc gcaaccgttg
     2041 ggatctcgct catgtacaag ctcatcagcc gccccggatg gggctcgatc atggcagagg
     2101 cgcagctcga ctggtacggg ctcgactaca cgatcgagga tgtcgacgac ctgttcaagt
     2161 ctgccgaggc cagggaaagc gttcagcgac tcaatcccct tgcccagctg ccgaccctta
     2221 tcctgccgag cggcgcggtg atgaccgaga gcgcggccat cacgctttat ctcgccgacg
     2281 tgacgagaag caacgaactc gtgcccgcgc ccgatgaact attaaggcca gccttcctgc
     2341 gctggctcgt gttcatcgtt gcgaacatct acccgacctt cacctacgcc gacgacccga
     2401 gccgttttgt gcctggcgag gaggcacaga aaggttttcg ggaaaatgtg gacgcctatg
     2461 cccgcaggct ctggggcatt ctcgaaagcg aggccggcca gccatggttc ctgggcgagc
     2521 gcctttctgc actcgatctt tacatctccg ccatgagccg ctggcggcca aaacgcgagt
     2581 ggttcgcggc gaatgccccg cgcctccacg caatcggaac ggcgttcgaa gccctgccgc
     2641 aagtgcagtc cgtctggcag cggaatttcc ccgcagctta aaccaacttc ggaatcggga
     2701 cacattgggc aggcccggtg tttatggatc aggcaagctg ccgatcggca aggaagcacc
     2761 tgatgcgcgc aagaaccata agcacccgcg gccgcaaggc ggaaggcctc tcggccctgc
     2821 cgctgccgcc ggatctgctg cccatggaag ccaagctcgt cgatcgcctg ccaacgggag
     2881 gcggctggca atacgagccg aaatggacga gcccattgat tttcctcatt tttttggccg
     2941 aaatgttctc acttctccag ttgttaatgg aaagtgagaa caaagcgttc tcgattccgc
     3001 cttggaccgc aggacgaatg cctcgtcgga aagccgtgcc cgatcagccg cctgggtata
     3061 ccgcagagcc tctttggcag ttttccatcc gaacacagct tgaagctgat tgaggttcgc
     3121 gcccttcaat gccaactcgc atgcggcagc cttcctgatg ccgtgtgccg agcaccccgc
     3181 aggcaagccg gcctcacggc accactccac aaagcgcctg ctgagtccct tatggctcgt
     3241 gtacggtggc ccccaatcgg ttgtcaggaa cgtcaggtcg ccgcacgggc ttttagcgat
     3301 caccgccagc agctcgggca tcgccggaat atcaattcga accggtgaac ggttacgatt
     3361 cttgtgagca gtgaaaacga tccgattgct gcgcacgtgc tgccgaccga gctgggcgag
     3421 gtcgctgacc cgacagccgg tccagtagag aatttcgaac gcgagccttg gtttcgtgcc
     3481 aacaggccac ttgtcgcgga attgctgcag ctcct
//
LOCUS       DASVDU010000290        17286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_655618, whole genome shotgun sequence.
ACCESSION   DASVDU010000290 DASVDU010000000
VERSION     DASVDU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17286
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655618"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2158
                     /locus_tag="VF226_19330"
     CDS             <1..2158
                     /locus_tag="VF226_19330"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX7076195.1"
                     /translation="DYYGRLIDGMRAERGRLRSGGDEGGGLAMQGSPPVEEIVSLFSG
                     IVRVGSDGLASVTFDLPEFNGTVRLMAVAWSADKLGHATADIIVRDPVAVLAAGPRFL
                     TLGDEARLQIDLHNVEGAAGPLSLTVEQESSSGLWSTIHQREVTLGLNERTAEHVSIK
                     PQSIGRFTYHIAVKGSDLDVQRTLTFDVKPPAGDIRRVTVSELAPGGSITLGPDLLAD
                     FIDGSARVSVSIGPAARLDVPGLLAQLDRYPYGCAEQTTSRALPLLYANQLAVQSGLP
                     RDAELKERIENAIARVFEMQDASGAFGTWGPGGGDMWLTSYVAEFLTRAREQGFAVKP
                     QGFELVLDRLQNFVSYAQDFERGGEARAYALYVLARNGRAPIGEVRYYADTRLDRFAT
                     PLAKAHLAAALAMLGDRERAESVFRQAAASVSQAEDALRRDFGSDLRDRAALVTLASE
                     TRVARNEAARLTATLAEAFARRAYTSTQEQAWLLLAARALADESRGTKLSVNGTPHEG
                     GLSRGLTSADLEKGQLVIRNEGAAAVDAVISVLGAAMTPEPAVARNFTIERAFYTLEG
                     TPIDLASADGGTGRLNQTDRLVVVLTVKAQANGHILIVDRLPAGLEIENPRLVDGGDL
                     TNFGWLKTAVQPEHTEFRDDRLVAAFDLTGRSNRDGQATNGSSGQRETTLTVAYVVRA
                     VTPGTFVHPAATVEDMYRPERFARTESGRLEVLAKD"
     gene            2193..4277
                     /gene="pbpC"
                     /locus_tag="VF226_19335"
     CDS             2193..4277
                     /gene="pbpC"
                     /locus_tag="VF226_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX7076196.1"
                     /translation="MRRWLLGAVSLCLIIVVGVFAAIRVTMIAIGPPPLEAAEQMSRT
                     VVDRDGNLLRAFTTPEGRWRLPVDPDDVDPRYLALLFAYEDGRYFLHGGVDPLAVVRA
                     GFQFLRNGRIVSGASTITMQVARLLEGRHERTLAGKWRQAVRAIQLENELSKDEILTL
                     YLRLAPFGGNLEGVRAASLAYFGKEPRRLSLGEAALLVALPQSPEWRRPDRHPQAARW
                     ARDRVLDRAVAEGVITKTEADRAREEPVPTKRREFPRFAPHISESEALERPNLQLHQL
                     TLSRELQGQLETLARDHARALGRRLSAAMLVVDNRTGEILAHVGSSDYLDSSRFGAID
                     MTGAIRSPGSTLKPIIYGLGFEAGLIHPETLIEDRPTRFGLYAPKNFDEDFRGSVTIR
                     EALAHSLNIPAVKVLNAVGPARLVVRFQKLGLQPVLPKHEQPTLAIALGGIGFSLSDL
                     ARLYTALARGGEAIALKHRLEPDDGGAASPAQTTRLLSPVAAWYVGDILKDAPPPAHA
                     AGGRFAYKTGTSYGYRDAWAIGYDGAHTIAVWVGRADGASTPGLTGRAAAAPLLFDAF
                     RRLGAKREPLPAPPAGVLQAGGAQLPPPLKRFAEPGDGRVATGPWLEPPVLIAFPPDR
                     SDLDVADADGIVILKAEGGALPLTWMVNGQPIPSPSHRREVVWEPDGRGFVKLTVTDA
                     KGRVDRSTIRLR"
     gene            4397..5470
                     /locus_tag="VF226_19340"
     CDS             4397..5470
                     /locus_tag="VF226_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HEX7076197.1"
                     /translation="MSQAHLTRLLAILLCLTLLAGCEKESSSRLPAVGARIDETSVSG
                     LSAGAYMAGQFQLAHGRDVVGVGIVAGGPWGCAESLYADMMPGPGTAFLNLSRAMNGC
                     MLNALQAWGIPDPRWLASRAGQLASSGRIAPIASVRQDRIYLFTGKEDHTVAPAIVVA
                     ARDFYLAIGVKDDHIRLVDDLPAGHAFVTEEHGLACDETGSPYIVDCNYDMAGAMLAH
                     FYGELRPKAEEPAGEVITFDQGEFLEGLTSHGMADTGAVYIAPDCRETAGCRVHIAFH
                     GCNQNVAAVGNAFINDSGFLAWADTNRLIVLFPQVSSTPANPQACWDWWGYTGRQFLT
                     RDAPQIVAVRRMLMRLAASPSMI"
     gene            5589..6554
                     /locus_tag="VF226_19345"
     CDS             5589..6554
                     /locus_tag="VF226_19345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076198.1"
                     /translation="MSAAHIALGRARVWLNWIDKDRLTALSALAALIVWISPNPFPAT
                     VQADLETRERARPAAPATSPASERATRREHRVGGYGGVSYTLPSAVVIQKPGSTDLTV
                     TDFQWIGRPFKAPIYYGLRTQHWLPGALTGTMVDFTHAKAIAQRDDVATFSGTLNGQP
                     VAPNAKIGDVFRHLEFSHGHNILTLNGLFRMPFSSWLPVRPYFGIGGGASFPHTEVGL
                     TSEPKRTYEYQFAGFAGQVLAGIEVPLGRVSLFFEYKFTYAPYSVPLSHEPNGWVFVT
                     DIWRQFRAWWTGEEPPGGILTTTLATHHAISGILVRTNTVQPAKK"
     gene            complement(6585..7400)
                     /locus_tag="VF226_19350"
     CDS             complement(6585..7400)
                     /locus_tag="VF226_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6456 domain-containing protein"
                     /protein_id="HEX7076199.1"
                     /translation="MHLQDGAAVPAWLRVLARRGSFLKLTPAGAEAFSPRNHFAKPIA
                     SVDRKRLAAAIEAGLLRDIGENRLGPTPRGIAALRAAFSEATAAKAAQPVEPKPAAPM
                     MNESESPLAWLRRRRDKEGKPLISGEQFDAGERLRRDFWFAQMTPKVTSSWDSPMSSR
                     RERRVAPGAGIELQDRVIAARERVRLALKAVGPELCGILIDVCCHLKGLEEAERSAGW
                     PQRSGKVVLQLALTRLARHYGILPAEEEPRRSPVRHWGAPDYRPSIDGGSPSS"
     gene            complement(7466..7756)
                     /locus_tag="VF226_19355"
     CDS             complement(7466..7756)
                     /locus_tag="VF226_19355"
                     /inference="COORDINATES: protein motif:HMM:NF019903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076200.1"
                     /translation="MLEETVARVFDVDLDLIYLPTRGRAKVAMARQVAMYLAHVSCGL
                     NLTEAGELFGRDRTTAAHACHVVEAKRENADFDRAIQLLENVTKVLMYTPPQ"
     gene            complement(8594..9004)
                     /locus_tag="VF226_19360"
     CDS             complement(8594..9004)
                     /locus_tag="VF226_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX7076201.1"
                     /translation="MDEMAKTELVGLTAEIVSAYVSNNTVVASDLPALIHDIHQALSR
                     ATSNSPPTEREELRPAIPVKRSVTPDYIICLEDGKKFKSLKRHLRTHYNLTPEEYREK
                     WGLPHDYPMVAPNYAAARSALAKKMGLGTRRDEG"
     gene            9622..10059
                     /gene="msrB"
                     /locus_tag="VF226_19365"
     CDS             9622..10059
                     /gene="msrB"
                     /locus_tag="VF226_19365"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7076202.1"
                     /translation="MQEKTTTRPEPTIVKSETEWRQQLTPEQFYVTRQHGTERAFTHP
                     YYGEKRRGMYHCVCCGAPLFSSEDKFDSGTGWPSFTRPINEGVVSEHEDRTLFMRRTE
                     VRCARCDAHLGHVFPDGPEPTGLRYCLNGVALQFRPEEEKPSK"
     gene            10139..12292
                     /locus_tag="VF226_19370"
     CDS             10139..12292
                     /locus_tag="VF226_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7076203.1"
                     /translation="MDAARAQKAALAADAQTTTPTPPRARKQPVRSKWHNVELVDEFA
                     WLRADNWQEVMRDPSVLPADIREYLEAENAYTEAVLADTRELQEELFREMKGRIKEDD
                     SSVPAPDGPWEYYTSYVTGGQYPLICRRPRGGGPEQVLLDGNKEAEGKAYWQLGGTGR
                     SPDHKLLAYAVDDKGSELYTVRIRDLDTGKDLPDIIVNTRGGMVWANDNRSLFYIRLD
                     EHHRPRFVCRHLVGTASEADATIYTEPDAGYYVGIGSTQSRKFIVIDAHDHQTNEVYL
                     IDSDKPDDEPRLIAARQTGHEYGVDHHDDQLIVTTNSGGAEDFRICVAPLSDPRMENW
                     RDLVPHKPGRLILEAIAFKDHLVRLEREDGLPRVVIRRFADGAEHAIAFDEEAYSLGI
                     TPGYEYDTTTLRFTYSSMTTPAQVYDYGMETRDRVLRKTQEVPSGHNPDDYVTKRLYA
                     PAPDGETVPISLLYRKGTPIDGSAPLLLYGYGSYGISIPANFSITRLSLVDRGFIYAI
                     AHIRGGKEKGYRWYKQGKHKTKRNTFTDFIAAGEYLASTGYTRRGRIVANGGSAGGML
                     VGAVANMAPDLFLGIIADVPFVDVLNTMLDKDLPLTPPEWPEWGNPIESAEDFELIRS
                     YSPYDNVERKVYPHIFAYGGLTDPRVTYWEPAKWVARLRDRKLGDNLVLLKINMDAGH
                     GGASGRFHQLREVALDYAFALKIAGLAGTKTPSSA"
     gene            complement(12384..12884)
                     /locus_tag="VF226_19375"
     CDS             complement(12384..12884)
                     /locus_tag="VF226_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEX7076204.1"
                     /translation="MDRADRRQRAARGRVAGCVLLAPFLLLAASKPASSERLENEVAV
                     FAALDKVTAQIDKLEVGLNETKQFGSLKITPRVCYSRPPTETPKTTTFVEIDEVQLNG
                     TEKRIFTGWMFAESPGLHPLEHPVFDVWLTGCKKPTTSVSQGGPAAGETPPGEFLEPP
                     RRRVRR"
     gene            complement(12974..13372)
                     /locus_tag="VF226_19380"
     CDS             complement(12974..13372)
                     /locus_tag="VF226_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEX7076205.1"
                     /translation="MGMLKEIFSWWSGNTWGTRLAIWTQGQFVGEDEFGNKYYVQKSG
                     VGPTGKPRRWVVYPSLSEPTQVPPEWFSWLHYTTDIPPTQESYQKRPWQKPHRMNMTG
                     TPQAYRPSGSIVGAGRPQKTRDDYKAWRPQ"
     gene            14103..>17286
                     /locus_tag="VF226_19385"
     CDS             14103..>17286
                     /locus_tag="VF226_19385"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX7076206.1"
                     /translation="MKITRRFTQAGRSPYENIPFRRANSEIKNPDGSIVFRLEGFMVP
                     EHWSQVAADILAQKYFRKAGVPSRLKRVEETQVPSWLWRSVPDEAALSELPADERFGG
                     ERDARQVFDRLAGTWTYWGWKGGYFTTEDDARAFFDEHRYMLAMQMGAPNSPQWFNTG
                     LHWAYGIDGPGQGHYYVDHETGRLTQSSSAYEHPQPHACFIQSIEDDLVNENGIMDLW
                     VREARLFKYGSGTGSNFSKLRAANEKLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVVVDVDHPDIEEYINWKVREEQKVAALVTGSKICQKRLKAVMKACVNCEAEGDA
                     CFDPAKNPALKRAIKEARRDQVPASYIARVIQFARQGFKDIEFETYDTDWDSEAYLTV
                     SGQNSNNSVRVTDDFLRAVLEDKEWGLTARTTGEVIKTVKARDLWDQIGYAAWASADP
                     GIQFHTTINDWHTCPQSGPIRASNPCSEYMFLDDTACNLASLNLMMFRQSADRQVGSR
                     APTADHQLPTALFDTDAFEHACRLWTIVLEISVLMAQFPSRQIAELSYRYRTLGLGFA
                     NIGGLLMASGIPYDSPEGRAICGAISAIMTGVAYATSAEMAKELGPFPGYFKNAEDML
                     RVIRNHRRAAYGEKDGYERLSTPPVPLDHVACPDKGLIEAAVRAWDRALELGEAHGYR
                     NAQATVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINQAVPQALRALGY
                     RESEIAEMEAYAVGHGSLRQAPAINHATLKAKGFTDEALQKIEDALATAFDIKFVFNR
                     WTLGDQFLTGTLKIPAEKLNDPGFELLTHLGFSKKDIEAANEHACGAMTLEGAPHLKP
                     EHLPVFDCANPCGRRGKRFLSVESHIRMMAAAQPFISGAISKTINMPNEATVEDCKQA
                     YLLSWRLGLKANALYRDGSKLSQPLNAQLLGDDEDEQEEVAEQLTADKPMAARSQVAA
                     ERIVERIVERARQREKLPNRRKSYTQKAIVGGHKVYLHTGEYDDGRLGEIFIDMHKEG
                     AAFRSLMNNFAIAVSLGLQYGVPLEEYVDAF"
BASE COUNT         3040 a         5600 c         5641 g         3005 t
ORIGIN      
        1 cgactactac ggccgcctca tcgacggcat gcgggccgaa cgcggccgcc tgcgctccgg
       61 aggcgacgag ggcggcggcc ttgccatgca gggcagcccg cctgtcgagg agatcgtgtc
      121 gctgttctcc ggcatcgttc gggtcggttc ggacggtttg gcctcggtca cgtttgatct
      181 gcccgagttc aacggcaccg tccggctgat ggcggttgcc tggagcgcgg acaagctcgg
      241 gcatgccacg gcggatatca tcgtgcgtga tccggtggcg gtgcttgccg ccgggccacg
      301 gttcctgacg ctcggcgacg aggcgcgcct gcagatcgac ctgcacaatg tcgaaggcgc
      361 ggctggcccg ctgagcctca cggtcgagca ggagagctcg tcaggtctct ggtctaccat
      421 tcatcagcgg gaggtgacgc tgggcctcaa cgagcgcacg gcagaacatg tcagcatcaa
      481 gccgcagagc atcgggcggt tcacctacca cattgccgtg aaggggagcg atctcgatgt
      541 gcagcggacg ctgacgttcg atgtcaagcc gcctgcgggc gacatccggc gcgtcacggt
      601 cagcgagctg gcgccaggcg gcagcatcac gctcggcccg gatcttcttg ccgacttcat
      661 cgacggctcg gcccgtgtct cggtcagcat cggaccggca gcgcgcctcg acgtccctgg
      721 cctgctggca caactcgatc gctatccata cggctgcgcc gagcagacga cgagccgcgc
      781 attgccgctg ctctatgcca accagctcgc cgtccagtcg ggcctgccac gtgatgccga
      841 gctcaaggag cgcatcgaga acgcgatcgc gcgcgtcttc gaaatgcagg atgcgtccgg
      901 cgcgttcggc acatgggggc caggcggcgg cgacatgtgg ctgacgagct atgtggcgga
      961 gttcctgacg cgggctcgcg agcaagggtt cgccgtgaag ccgcagggct tcgagttggt
     1021 tctcgaccgg ctgcagaact tcgtcagcta cgcgcaggat ttcgagcgtg gtggcgaggc
     1081 gcgggcttac gcgctctacg tcctggcgcg caacggccgc gcaccgatcg gcgaggtccg
     1141 ctattacgcc gacacccggc ttgaccggtt tgccactccg ctcgcaaagg cgcatctggc
     1201 cgctgcgctt gccatgctgg gtgatcggga gcgcgccgag tcggtgttcc ggcaggccgc
     1261 ggcctccgtg tctcaggccg aggacgcctt gcggcgggat ttcggctcgg atcttcgtga
     1321 cagggcagcg ctggtgacgc tcgcatctga aacccgcgtg gcgcgcaacg aggccgcgcg
     1381 gctcaccgcg actctcgccg aggcgttcgc ccggcgggct tacacctcca cgcaggagca
     1441 ggcctggctt ctgctcgccg cccgggcgct tgccgatgag agccgcggca ccaagctctc
     1501 cgtcaacggc acgccacatg aaggtggact cagccgaggc cttacttcgg ctgatctcga
     1561 aaagggccag ctcgtcattc gcaacgaggg cgcggcagcc gtcgacgccg tgatctcggt
     1621 cctcggcgcc gccatgactc cggagccggc ggttgcgcgc aacttcacaa tcgagcgcgc
     1681 gttctacacc ctggagggca caccgattga tctggcaagt gccgacggcg gaacaggccg
     1741 gctcaaccag acagatcgtc tcgtcgttgt cctcacggtc aaagctcagg caaacggtca
     1801 catactgatc gttgatcgct tgccggcggg cctcgagatc gagaatccac ggctcgtgga
     1861 tggcggcgac ctgacgaact tcggctggct gaagacggca gtccagcccg agcataccga
     1921 gttccgggac gaccggctgg tcgcggcgtt cgacctgacc gggcggagta atcgagacgg
     1981 ccaagcgacg aacggctcgt ctggccagcg ggaaacgaca ctgaccgtcg cttacgtggt
     2041 gcgcgccgtc acgccgggga cgttcgtgca tccggcggcc acggtcgagg acatgtaccg
     2101 gcccgagcgg ttcgcccgta cggaatcggg aagactggag gttctggcga aggattgaag
     2161 gcgtggagaa ggcgacgaaa gctaggggga ggttgagacg ctggctgctc ggcgcggtgt
     2221 ccctctgctt gatcatcgtg gtcggggtct ttgctgccat ccgagtgacc atgatcgcca
     2281 ttggcccgcc gccgctcgag gcagccgagc agatgtcccg aaccgtcgtc gatcgcgatg
     2341 gcaacctgct gcgggccttc accacccccg aaggccgttg gcgcctgccg gtagaccccg
     2401 acgacgtcga tccacgctat ctcgccctgc tgttcgccta cgaggacggc cgatattttc
     2461 ttcatggcgg cgtcgatccg ctggccgtcg tcagggctgg ctttcagttc ctccgcaatg
     2521 gccgcatcgt gtccggcgcc tcgacgatca ccatgcaagt ggcgcgcctc ctcgagggca
     2581 ggcacgagcg gacgctcgcg ggcaagtggc ggcaggccgt ccgcgccatc caactcgaga
     2641 atgagctttc gaaggacgag atcctgaccc tttatctgcg gctggcgcca ttcggcggca
     2701 atctggaggg agtgcgcgcc gcctcgctcg cctacttcgg caaggagcca cgccggttgt
     2761 cgctcggcga ggcagcactg ctggtcgccc tgccgcagtc gcccgagtgg cggcggcccg
     2821 atcggcatcc gcaggcggca cgctgggcgc gtgatcgtgt tcttgaccgg gccgtcgccg
     2881 aaggcgtcat caccaaaacg gaggccgacc gcgcccgcga ggagcccgtg ccgacaaagc
     2941 ggcgcgagtt cccgcggttc gcgccgcaca tttcggagag cgaagctcta gagcggccca
     3001 atcttcagct gcatcagctc accttgtcgc gcgagctaca gggccagctc gagacgcttg
     3061 cccgcgacca tgcgcgggcg ctgggacggc ggctttcagc ggcgatgctg gtggtcgaca
     3121 acaggaccgg cgagatcctg gcccatgtcg gctcgtcgga ttacctcgac tcctcccgct
     3181 tcggggcaat cgacatgacc ggtgcgatcc gctctccggg gtccaccctg aagccgatca
     3241 tctacggcct cggcttcgag gccggcctca tccatccgga aaccctgatc gaggaccggc
     3301 cgacccgctt cggactttat gccccgaaga atttcgatga agacttccgc ggttccgtaa
     3361 cgatacgcga ggcgctggcg cactcgctga acattccggc ggtcaaggtg ctgaacgccg
     3421 tcggtccggc gcgtctggtg gtgcggttcc agaagctcgg gcttcagccg gtgctgccga
     3481 aacacgagca accgacgctt gcgatcgccc tcggcgggat cggcttcagc ctgagtgatc
     3541 tcgcacgcct ttacacggcg ctcgcccggg gcggagaggc gattgcgctg aaacatcgtc
     3601 tggagccgga cgacggtggc gccgcctcgc cggcgcagac cacgcggctg ctgtcgccgg
     3661 tggcggcctg gtacgttggt gacatcctca aggatgctcc gccgccggcc cacgccgcgg
     3721 gcgggcgttt tgcctacaag acgggcacgt cctacggcta ccgcgatgcg tgggccatcg
     3781 gctacgacgg tgcgcacacc atcgcggtat gggtcgggcg tgcggatggc gcctccacac
     3841 caggacttac gggccgcgcg gcggccgccc cccttctgtt cgatgccttc cgtcggctcg
     3901 gggccaagcg cgagccgctg ccggcgcctc cggcgggcgt tctgcaggct ggaggggcgc
     3961 agctgccgcc gccgctcaag cggttcgccg agccggggga cgggcgcgta gcgacgggac
     4021 cctggctcga gccgccggtg ctgatcgcgt ttcctccgga tcgttcggac ctcgacgtcg
     4081 ctgacgccga cggcatcgtg atcctcaagg cagagggtgg cgcacttcct ctgacgtgga
     4141 tggtgaacgg ccagcccatt ccgtccccct cgcaccggcg cgaggtggtc tgggaacctg
     4201 acggccgggg gttcgtgaag ctcaccgtga ccgacgccaa gggccgggtc gacaggtcaa
     4261 ccatccgcct gcgttgacgg cgaaggcgcg agactgtttc attttctcaa ctctgcaatg
     4321 caaatccaag aggtgcggtc tggccaatcc gcagctcgcc acggtaggct ctacgcaggg
     4381 cttagagagg caccgcatga gccaagcgca cctcacccgc ctgcttgcta ttctgctctg
     4441 tctgacactg ctcgccggct gcgagaagga aagcagcagc agattgccag cggtgggcgc
     4501 gcgcatcgac gagacgtccg tatctggcct gtcggccggt gcctacatgg ccgggcagtt
     4561 tcagctcgcc cacggccgcg atgtcgtggg cgtcggtatc gttgctggcg gaccctgggg
     4621 gtgtgcggaa agcctctacg ccgacatgat gccggggccc ggcacggcat tcctcaacct
     4681 ctcccgggcg atgaacggct gcatgctcaa tgccctgcag gcctggggca ttcccgatcc
     4741 gcgctggctc gcaagccgcg ccggccagct cgcctcatcg ggccggatcg ctccgatcgc
     4801 ctccgttcgc caggaccgta tctatctctt taccggcaag gaggatcaca ctgtcgcgcc
     4861 ggccatcgtg gttgctgcaa gggatttcta tctggccatc ggcgtaaagg acgaccacat
     4921 caggcttgtc gacgacctgc ccgcgggcca cgcgttcgtg accgaggagc atggcctcgc
     4981 ctgcgatgaa accggctcgc cctatatcgt cgactgcaat tacgacatgg ccggagcaat
     5041 gctcgcgcac ttttatgggg agctcaggcc gaaagcggag gaaccggctg gggaggtcat
     5101 caccttcgac cagggtgaat tccttgaagg tctgaccagc cacggcatgg ctgacaccgg
     5161 ggcggtgtac attgctcctg actgccgcga gacggccgga tgccgggtcc acatcgcgtt
     5221 ccacggctgc aaccagaacg tcgcggctgt gggcaatgcg ttcatcaacg acagcggttt
     5281 cctcgcctgg gccgacacga accggctaat cgtgctcttc ccccaggtca gcagcacgcc
     5341 ggccaacccc caggcctgct gggactggtg gggctacacg ggccgccagt tcctcacgcg
     5401 ggatgctcca cagattgtcg cagtgcgacg catgctgatg cgtctggcgg cgtcgccctc
     5461 gatgatttag agcaaagccc acgacgcccg cgcgtgcgca gcgaaattgc ccagccgtac
     5521 gggacgagtg agggaatgtt cgcggcatcc aatgccgggg ctaatgggga tcggaggggg
     5581 accagatcat gagcgctgcg catatcgcgc tgggccgcgc cagggtatgg ctcaactgga
     5641 tcgataaaga tcggttgacg gcgctctctg cgctcgccgc gctgatcgtt tggatctcac
     5701 ccaatccatt cccggcaact gtccaggcag acctcgagac ccgggagcgg gcgcgcccgg
     5761 cggcgccggc cacgtcgcct gcctctgagc gcgcgacgcg gcgcgagcac agggtcggcg
     5821 gctatggcgg tgtttcctat acgcttccga gcgccgtcgt tatccaaaaa ccgggctcta
     5881 cggatctgac cgtaacggat ttccagtgga tcgggcggcc gttcaaggct cccatctact
     5941 atggcttgcg cacccagcac tggctgccag gcgcgctgac cggcacgatg gtcgacttca
     6001 cgcacgccaa ggcgattgcc cagagggacg atgtcgcgac cttctccggc acgctgaacg
     6061 gtcagcccgt ggctccgaat gcgaagatcg gcgatgtctt ccggcatctg gagttcagtc
     6121 acggccacaa tattctgacc ctcaatggcc tcttccggat gccgttctcc tcgtggctgc
     6181 ccgtgcggcc ctatttcggg atcggcggcg gggcttcgtt cccgcatacg gaggtcggcc
     6241 tgacgtccga gcccaagcgc acctacgagt atcagtttgc cggtttcgct ggccaggtgc
     6301 tggccgggat cgaggtcccg ctggggcgcg tctccctgtt cttcgagtac aagttcacct
     6361 acgcgcccta cagcgttccg ctctcgcatg aaccgaacgg ctgggtgttc gtcacggata
     6421 tctggcgcca gttccgggcg tggtggaccg gcgaggagcc gccgggcggt atactgacga
     6481 cgacgctcgc gacccatcac gccatcagcg gtattctcgt gcggaccaac accgttcagc
     6541 cggcgaagaa gtagtccgcg agactaccgc agcctgccac cccgctacga tgacggcgag
     6601 ccaccgtcga tgctcggcct gtagtctggc gcaccccaat ggcgcacggg cgaacgcctg
     6661 ggttcttcct cggctgggag aatgccgtag tggcgcgcca gccgggtcaa tgcgagctgc
     6721 aggaccacct tgcctgagcg ctgcggccag ccggctgatc gctccgcttc ttccagccct
     6781 ttcaggtgac agcagacgtc tatcaggatg ccgcagagct ccgggccgac tgccttcagt
     6841 gccagtcgca ctcgctcgcg cgcggcgatc acgcgatcct gcagctctat tcccgctccc
     6901 ggtgctactc gcctctcccg gcggctggac attggtgaat cccagctcga cgtcaccttc
     6961 ggggtcatct gagcgaacca gaaatcgcgc cgcagccgct cgcccgcatc gaactgctcc
     7021 ccggagatga ggggcttgcc ttccttgtcg cggcgccggc gcagccaggc gagcgggctt
     7081 tccgactcgt tcatcatcgg cgcagccggc ttcggctcga ccggttgcgc cgcttttgcg
     7141 gcggttgcct cgctgaaagc ggcacgcagg gctgcgatac cccgaggtgt ggggccgagg
     7201 cggttctcgc cgatatcgcg caggagtccc gcttcgatcg cagccgcaag ccgcttgcga
     7261 tcgacgctgg cgatcggctt ggcgaagtga tttcgcggcg agaaagcctc ggcaccggca
     7321 ggggtcagct tgaggaacga gccccggcgc gcgagcacgc gcaaccatgc ggggacggcg
     7381 gcgccatcct gcaaatgcat tctggaaacc tcatcagtaa cgggaatgtc ggtctgacgg
     7441 caacgagtgc tcggtgcgag ctcgctcact gcggcggcgt gtacatcaga accttcgtca
     7501 cgttctcgag cagctggatc gcccgatcga agtccgcgtt ttcgcgcttt gcttcaacga
     7561 catggcaggc atgcgccgcg gtcgtgcgat cacggccgaa cagctcgccg gcttcggtca
     7621 ggttcaagcc gcacgagacg tgcgcgaggt acatggccac ttggcgcgcc atggcaacct
     7681 tggcgcggcc tcgggtcggc aggtaaatca ggtcgaggtc cacgtcgaag acgcgggcca
     7741 cggtctcctc gagcatcact ctcagctcct gcgtggccga gcggctcctg gatcgctttc
     7801 gcgaccggcg ctcggtcgga caggattgcg gcgatgatgc ggtaacgtac atgtcagccc
     7861 ccaaactgca cttggccagg gtgcgccccg gctcattgga ggccaaagga tattggctcc
     7921 acctccgggg caccgggacc atgttaaggt ttaggtgaac ggcggggata agtcgggcta
     7981 gggttaacgg ccggaccgca gcctttaccg tccgacaaaa gccgactcgc ctctgcgccc
     8041 aaaaaagtcc ggctactgaa cagctgaaaa gttatccccg ccagcccgag cggtgcgaag
     8101 ctgtcctctc attcaggtga ggagaggacc atgccagaag cagcgacgtc agtgcccgcc
     8161 gggcggcgag gcggaagaag cgcgagaacc gtccgtaggc cgggggggtc cgcgccacgc
     8221 gatcgcactg tcaaaccagc ttcctacgac cggaggaagg acttgccggg cctgctggcg
     8281 ctgtggccct gggagatcga ggacacgacc tgcgaaggac atcgccgatt ggtcgctctc
     8341 ttgcgcaggg cgttgcgcct cgagaggcag cgcggcatgt ctgggcactg gacctatgac
     8401 gtcgcgcgcc atgcgcgcct gctcagggcc tacaagtgcg aagccgagag tctgagatct
     8461 cggcttcggg acaacgccaa gaagaacaag aatgtcgtct tgcccggtga gtttctgccg
     8521 atcgatggca cttggtccgg ttcgagggcg gcgccggcgc cagttgagga gaagccgttt
     8581 gaggagaaac cctctagcct tcgtcccgcc tcgtgccgag gcccatcttc ttggccaggg
     8641 cagaacgggc ggccgcgtag ttcggcgcta ccatcgggta gtcgtgcggc aggccccact
     8701 tctcccggta ttcctccggg gtcagattgt agtgggtacg cagatggcgc ttcagcgact
     8761 tgaatttctt accgtcctcg aggcagatga tgtagtcggg tgtcaccgag cgcttgacgg
     8821 ggattgcggg ccgcaactcc tcccgctccg tcgggggaga attggacgtg gcgcggctga
     8881 gggcctgatg gatgtcgtgg atcagagctg gcagatctga agcgacaacc gtgttgttgc
     8941 tcacataagc cgagacgatc tcagccgtca gcccgacgag ttcggttttc gccatctcgt
     9001 ccattctctt ctccatagtc aaacccccgg aagtctgctt ggcagcagag gacatggctc
     9061 taaggcaggt cgatctccat gaccctcaag gctgaccaag tctacttgtc cccgaattcc
     9121 tgcaacggca gtgcaggttg cagatcatgg acaacagaaa tgccgaattg tcaatactgc
     9181 ccgaagctga aacaggacat ttcccaacta aactgataac ggttgcacgg tcataattcg
     9241 agggataata agtattactc gcatccaagc cgcagatgag acaccatact gacgagttgt
     9301 cgccggcggc ggtctcgcag aactgcagaa cttgcgatcg gcgccgtaaa cgattgtcga
     9361 gactcgggcc aaatcaaggc aggggcttgc ctgctggcgc accctctccg ggcggaattg
     9421 aacccggtgg gcttgtctgc cgttgatgca tcgggtgcgg gccccaggcg agaaaagcga
     9481 actggagaga agggccagct gctgtgttcc gccatcggga gcatatgtgt aacccgtcgc
     9541 cgggtgcata tgtgtagttt gtgccgatgt gccccgcgcc gtgccgaaag gccggctgaa
     9601 gaggagacag ggagtttgca gatgcaggag aagacaacca cccggcccga acccacgatc
     9661 gtcaaatccg aaaccgagtg gcgccagcaa cttactcccg aacagttcta cgtaactcgc
     9721 cagcacggca ctgagcgcgc cttcacgcat ccctactatg gcgagaagcg ccgaggcatg
     9781 tatcattgcg tctgctgcgg agctccgctc ttctcgtccg aagacaagtt cgactccggt
     9841 acgggttggc ccagtttcac ccggcccatc aacgaggggg tcgtcagcga gcacgaggac
     9901 cggacgctct tcatgcggcg gaccgaggtg cgctgtgcgc gctgcgatgc gcatcttggg
     9961 cacgttttcc cggacggccc ggagccgaca ggactacgct actgcctcaa cggcgtggcg
    10021 cttcaattca ggccagaaga agaaaaaccg agcaaatagc acgcgatcgc accgccggct
    10081 tgacttgcgt ttgaactgcg gtagcgggcg gaaacatcac aaccggagta ctttgcacat
    10141 ggatgctgct cgcgcccaga aagcagccct ggctgctgac gcccagacga caactcccac
    10201 cccgccacgc gccagaaagc agcctgtcag gtcgaagtgg cacaacgtcg agcttgtcga
    10261 cgagttcgcc tggctgcgcg ccgacaactg gcaggaggtg atgcgggacc catccgtgct
    10321 gccggccgat atccgcgagt acctcgaggc ggagaacgcc tatacggagg ctgttcttgc
    10381 ggacacacgc gagctgcagg aggaactttt ccgggagatg aagggccgca tcaaggagga
    10441 cgacagctcc gtcccggcgc cggatggacc ctgggagtac tacacgagct atgtgacggg
    10501 cgggcagtat ccgctgatat gccgccggcc ccgcggtggt gggcccgagc aggtgctcct
    10561 cgacggcaac aaggaagcgg aggggaaggc ctattggcag ctcggcggca cggggcgcag
    10621 tccggaccac aagctgctgg cctatgcggt cgacgacaag ggctccgagc tctacacggt
    10681 ccggattcgc gacctcgata ccggcaagga cctgcccgac atcatcgtca acacgcgcgg
    10741 cggcatggtc tgggcgaacg acaatcgctc gctgttctac attcggctcg acgagcacca
    10801 tcgtccgcgc ttcgtctgcc gccatctcgt cggcaccgcg agcgaggcgg acgccacgat
    10861 ctacacggag ccggatgcgg gctattacgt cggcataggc tccacgcagt cgcgcaagtt
    10921 catcgtcatc gatgctcacg atcatcagac gaacgaggtc tatctcatcg acagcgacaa
    10981 gcccgatgat gagccgcggc tcattgcggc ccgccagacc ggtcacgaat acggcgtcga
    11041 ccatcatgac gatcaactga tcgtcacgac caattccggg ggtgcggagg acttccgcat
    11101 ctgcgtcgct ccgctcagtg acccgcgcat ggagaactgg cgggatctcg tcccgcacaa
    11161 gccgggacgt ctcatcctgg aggcgatcgc attcaaggat cacctcgtgc gactcgagcg
    11221 ggaggacggg ctgcctcggg tcgtgatccg ccggttcgcc gatggcgcgg agcatgccat
    11281 cgctttcgac gaggaggctt actccctcgg catcactcct ggctacgagt acgatacgac
    11341 gacgttgcgc ttcacgtact cgtccatgac cacgccggcc caggtctacg actacggcat
    11401 ggagacccgc gaccgggtgc tgcgcaagac acaggaggtg ccaagcgggc acaacccgga
    11461 cgactatgtc acaaagcgcc tttatgcgcc cgcgccggac ggcgagacgg tgccgatcag
    11521 cctgctttac cgcaagggca ccccgatcga cggatcggcg cccttgctgc tttacggata
    11581 cggcagctac ggcatttcga tcccggccaa tttctcgata acacggctgt cactggtcga
    11641 ccgcggcttc atctatgcca ttgcccatat ccgcggcggc aaggagaagg ggtaccgctg
    11701 gtacaagcag ggcaagcaca agacgaagcg caacacgttc accgatttca tcgccgccgg
    11761 cgagtatctc gcgtcgacgg gctatacccg gcgcgggcgg atcgtcgcca acggcggctc
    11821 ggcgggcggc atgctggtcg gcgccgtggc gaacatggcg cccgacctgt tcctcggcat
    11881 catcgccgac gtgccgtttg tcgatgtgct caatacgatg ctcgacaagg acctgccgct
    11941 gaccccgccc gaatggcccg agtggggcaa tccgatcgag tcggcggagg acttcgagct
    12001 gatccgttcc tacagcccgt acgacaacgt cgagcgtaag gtctacccgc acatcttcgc
    12061 ctatggcggc ctgaccgatc cgcgcgtcac ctactgggag ccggccaagt gggtcgcccg
    12121 cctgcgtgac cgcaagctcg gcgacaatct cgtgctgctc aagatcaaca tggacgccgg
    12181 ccacggcggc gcttccggcc gcttccacca gctgcgcgag gtggcgctcg actacgcgtt
    12241 cgcgctgaag atcgccggac tcgcaggcac gaagacccca tcctcggcat aggctctgcc
    12301 cgagcaatcg tccgggtgcg agcctcgtgt cgctctggcg cgctgcgaga cggcgggccc
    12361 gccaaagggc cgcgtcccaa tgttcagcgg cgcacgcggc ggcggggcgg ttcaaggaac
    12421 tcgcctggag gtgtttcgcc cgcggcgggg ccgccctggg aaaccgatgt ggtcggcttt
    12481 ttgcagcccg tcagccatac gtcgaaaacc gggtgctcga gggggtgcaa gccgggactc
    12541 tcggcgaaca tccagccggt gaagatgcgc ttctccgtac cgttgagctg cacctcgtcg
    12601 atttcgacga atgtcgtcgt cttgggcgtt tcggtcggcg gccgcgagta gcacacccgc
    12661 ggtgtgatct tcagcgatcc gaactgcttg gtctcgttga ggcccacctc gagcttgtcg
    12721 atctgagcgg tgaccttgtc gagcgcagcg aagacggcga cctcgttctc gaggcgttcc
    12781 gagcttgccg gttttgatgc cgccaagagg agaaacggcg cgagcagcac gcacccggca
    12841 acccggccgc gcgcggctcg ctgccggcga tccgcacgat ccatgccctc tcacccgcta
    12901 ttatgagcca acacgcggaa ggagtgcaca gccaaacttg gcgtcaccat gacaatggcc
    12961 ggcagatccc gggttattgc ggccgccacg ccttgtagtc gtccctcgtc ttctggggtc
    13021 ggcccgcacc aacgatggag cctgacggcc gataggcctg tggcgtgccg gtcatgttca
    13081 tgcgatgcgg cttctgccag ggccgtttct gatagctttc ctgggtgggc ggtatgtcgg
    13141 tcgtgtagtg cagccagctg aaccactcgg gcggcacctg cgtcggctcg gagagcgagg
    13201 gatagaccac ccagcgccgc ggcttgccgg tggggccgac gcccgatttc tgcacgtaat
    13261 acttgttgcc gaactcgtcc tcgcccacga actggccttg ggtccagatg gcgagcctgg
    13321 tgccccaggt attcccgctc caccaactga agatctcttt cagcatgccc atgtcagtca
    13381 gcccgtgacg aatgccgtgt gcctgatgcc tcgctccagc gatcccgcgg cataacgtac
    13441 gctccaaggc gtgtccagtc ggcggattgg cgctgcggtt tggccacacc aggtgtgccc
    13501 tgaatcggca tagcatccgg ggacggcgcc gatcccgcgg ccctcgagag aatctgggga
    13561 gatcggtgcg ccgggcgcag atgcggtatg gacgctccgt cgtttccggc cgtcgcatga
    13621 tcgtgagccc gctttgcacc cggaccatcc accggttgtc caccagcctt gcacaacgct
    13681 tactacatgt agcggcgctt gcatgtacgc cccactacat cgtgacaaat cagctcgaat
    13741 caacgaaggt ttgctcaagt cggtcgcggt gctgtcacct gcggcaccgc ggcaggaggg
    13801 acataggggc ccgctcgggg agagtttggt gagcgggccc ttctcgttga aagcgttgcc
    13861 tgtcggactg cgggggtaga ggtcttcgcg tgctttcgaa gccgcgcttc ctagtttgcg
    13921 ttcccgagta atccacaagc ccggagcgta gtccgggtcc cacggccggt gcctgatcgc
    13981 gctggctgac gaattcgctt gcgccggcgc atccgcgtgc ggcgctccga agaaacgatg
    14041 gtgccggcgc gatcgacagg gggtcgcggg gcgtcgagag cagatgagcg ggggctataa
    14101 gaatgaagat cacgcggcga ttcacgcagg ccggtcggtc gccttacgaa aacatcccat
    14161 ttcgtcgtgc caacagtgag atcaagaacc cggacggctc catcgtcttc cggctcgagg
    14221 gcttcatggt acccgagcac tggagccagg ttgcggcgga cattctcgcg cagaagtact
    14281 tccgcaaggc gggcgtcccg agccgactga agcgggtcga ggagacacag gtcccgtcct
    14341 ggctctggcg gtcggtgccg gatgaggcag cgctcagcga gctgcccgct gacgagcgct
    14401 tcggcggcga gagggatgcc cggcaggtgt tcgaccggct ggccggcacg tggacctact
    14461 ggggctggaa gggcggctac ttcaccaccg aggacgacgc ccgcgccttt ttcgacgagc
    14521 accgctacat gctcgccatg cagatgggcg cgccgaacag cccgcagtgg ttcaacacgg
    14581 ggctgcattg ggcctacggc atcgacgggc cggggcaggg gcactactac gtcgatcatg
    14641 agaccggcag gctgacgcag tccagttccg cctacgagca tccccagccg cacgcctgct
    14701 tcatccagtc gatcgaggac gatctcgtca acgagaacgg gatcatggac ctctgggtgc
    14761 gtgaggcgcg gctcttcaag tacggctcgg gcaccggcag caacttctcc aagctgcgcg
    14821 cggcgaacga gaagctgtcc ggcggcggac gttcgtccgg cctcatgagc ttcctcaaga
    14881 tcggtgaccg agcggcgggc gccatcaagt cgggcggcac aacgcggcgc gcggcgaaga
    14941 tggtcgtcgt cgacgtcgat caccccgaca tcgaggaata catcaactgg aaggtacgcg
    15001 aggagcagaa ggtcgccgcc ctcgtcaccg gctccaagat ctgccagaag cggctgaaag
    15061 ccgtgatgaa ggcctgcgtc aactgcgagg ccgagggcga cgcctgcttt gatccggcaa
    15121 agaacccggc gctgaagcgc gccatcaagg aggcgcgccg cgaccaggtg ccggcgagct
    15181 acatcgcccg cgtgatccag ttcgcgcgcc aaggcttcaa ggacatcgag ttcgagacct
    15241 acgacacgga ctgggacagc gaggcttacc tcacggtttc cggccagaac tcgaacaatt
    15301 ccgtccgcgt caccgacgac ttcctgcgcg ccgtgctgga ggacaaggag tggggtctga
    15361 cggcgcgcac cacgggcgag gttatcaaga ccgtcaaggc ccgcgatctg tgggatcaga
    15421 tcggttatgc cgcctgggcc tcggccgatc ccggcatcca gttccatacc accatcaacg
    15481 actggcacac gtgcccgcag tccgggccga tccgcgcgtc caatccgtgc tcggagtaca
    15541 tgttcctcga tgatacggcg tgcaatctgg cgtcgttgaa cttgatgatg tttcggcagt
    15601 cggcagatcg gcaggtcggc agtcgggctc ccactgccga tcaccaactg ccgactgcgc
    15661 tattcgacac ggatgccttc gagcatgcgt gccgcctctg gaccatcgtg ctcgagattt
    15721 cggtgctgat ggcgcaattt ccgagccggc agatcgcgga gctgtcctat cgctaccgca
    15781 cgctgggtct cggcttcgcc aacatcggcg gtctgctgat ggcctccggc attccctatg
    15841 actcgcccga gggccgcgcc atctgcggtg ccatctcggc gatcatgacg ggcgtggcct
    15901 atgccacctc ggcggaaatg gccaaggagc tcgggccgtt ccccggctac ttcaagaacg
    15961 ccgaggacat gctgcgcgtc atccgcaatc atcgccgcgc ggcttatggc gagaaggacg
    16021 gctatgagcg actttcgacg ccgccggtgc cgctcgacca tgttgcgtgt ccggataagg
    16081 ggctgatcga ggccgccgtg cgcgcctggg accgggcact ggagcttggc gaagcgcacg
    16141 gctaccgcaa tgcgcaggcg accgtcatcg cgccgacggg caccatcggt ctcgtcatgg
    16201 attgcgacac gacaggcatc gagcccgact tcgcgctggt gaagttcaag aagttggccg
    16261 gcggcggcta cttcaagatc atcaatcagg cagttccgca ggcgcttcgc gcgctgggct
    16321 accgcgaatc cgagatcgcc gaaatggaag cctacgcggt cggtcacggc tcgctcaggc
    16381 aggccccggc catcaaccac gccacgctca aggcgaaggg cttcaccgac gaagcgctgc
    16441 agaagatcga ggacgcgctg gcgacggcgt tcgacatcaa gttcgtgttc aaccgctgga
    16501 cgctgggcga ccagttcctg acgggcacgc tcaagatccc ggccgaaaag ctcaacgatc
    16561 ctggcttcga gctgctcacg cacctcggct tctccaagaa ggacatcgaa gccgccaacg
    16621 agcacgcttg cggggccatg actctcgagg gcgcgccgca cctcaagccg gagcatctgc
    16681 ccgtcttcga ctgcgccaac ccgtgcgggc gccgcggcaa gcgattcctg tcggttgaga
    16741 gccacatccg catgatggcg gcggcgcagc cgttcatctc gggcgccatc tccaagacca
    16801 tcaacatgcc gaacgaggcg acggtcgagg attgcaagca ggcctacctg ctgtcgtggc
    16861 ggctggggct caaggccaac gcgctctacc gcgacggctc gaagctgtcg cagccgctca
    16921 atgcgcagct ccttggcgat gacgaggacg agcaggagga ggttgccgag cagctcacgg
    16981 ccgacaaacc gatggcggcg cgcagccaag tggcggccga gcgcatcgtg gagcgcatcg
    17041 tcgagcgggc ccgccagcgc gagaagctgc ccaaccgccg caagagctat acgcagaagg
    17101 cgatcgtcgg ggggcacaag gtttatctgc acaccggcga gtacgacgat gggcgtctcg
    17161 gcgagatttt catcgacatg cacaaggagg gcgccgcctt ccgctcgctg atgaacaact
    17221 tcgccatcgc ggtcagcctc ggcctgcagt acggcgtgcc gctggaggag tacgtcgacg
    17281 cgttca
//
LOCUS       DASVDU010000291        30427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_464934, whole genome shotgun sequence.
ACCESSION   DASVDU010000291 DASVDU010000000
VERSION     DASVDU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 30427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30427
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464934"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..956)
                     /locus_tag="VF226_19390"
     CDS             complement(45..956)
                     /locus_tag="VF226_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076207.1"
                     /translation="MTIFVGLLAGLLIAPAVLYPVFLMKVLCFALFALAFNLLIGYVG
                     LLSFGHAMFFGFAAYISAYTVKAWGWPTELGILAGTGVAMLLGVVAGFIAIRRQGIYF
                     AMVTLALAQMVFFFCLQAPFTGGEDGIQAVPRRPVFGVIDITDDLTFYYVVLAIFAAG
                     FLIIYRAIYSPFGQVLKAIRENEQRAVSLGYHVDQYKLLAFVLSATLSGLAGATKAIV
                     FQLASLTDVMWQMSGEVVLMTLLGGMGTVFGPVVGAFTIISLEHYLADRGQWVTVITG
                     AIFVVCVLAFRRGIVGELAAWWARRRG"
     gene            complement(1051..1932)
                     /locus_tag="VF226_19395"
     CDS             complement(1051..1932)
                     /locus_tag="VF226_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076208.1"
                     /translation="MFELIGIPPQALFGQLLLGLINGAFYALLSLGLAVIFGMLNVIN
                     FAHGAQYMMGAFTAYMLLQYLGVGYWGALILTPIIIGAFGILLERLFLSRLYHLDHLY
                     GLLLTFGLALIIEGVFRQQYGSSGLPYTNPIPGGTNLGFMFLPWYRGWIVVLSLVVCL
                     ATWFMIEKTKLGSYLRAATENPTLVRAFGINVPRMMTLTYGFGVGLAALAGVLAAPIY
                     SVNPVMGANLIIVVFAVVVIGGMGSIMGSILTGFGLGVIEGLTKVFYPEASNTVIFII
                     MAIVLMLRPAGLFGTPR"
     gene            complement(2039..3232)
                     /locus_tag="VF226_19400"
     CDS             complement(2039..3232)
                     /locus_tag="VF226_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076209.1"
                     /translation="MAAAASVLATAAHAQFTDGVIKIGVLNDQSGVYADLSGQGSVWA
                     ARKAVEDYCKANKCHDKIEVIFGDHQNKPDIGANIARQWYDVEGVDVIVDVPTSSVAL
                     AVHNITREKNKVHLNSTGATSDLTGKDCSPNTIHWTYDTWSLANGTGKAVVKTGGDTW
                     FFLTADYAFGHALERDTAAVVEANGGKVLGKVRHPFPGQDFSSFLLQAQSSGAKIIGL
                     ANAGGDTINSVKQAAEFGITQGGQNLAALLMFITDVHALGLKTAQGLIFTEAWYWDLN
                     DNNRAFAKEFEVDNKGHKPTMVQAGVYSAVTHYLKAVHALGSDADGAKVVAKMKEMPT
                     EDRLFGKGIIRPDGRKIHDMYLFEVKKPEESKGPWDYYKLRATIPANEAFRPLSEGGC
                     PLVKS"
     gene            complement(3286..3999)
                     /locus_tag="VF226_19405"
     CDS             complement(3286..3999)
                     /locus_tag="VF226_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076210.1"
                     /translation="MPEPTAPLLSVRDLHAWYGESHVLHGMTFDVSKGELVTLLGRNG
                     VGKTTTMRAIMGIVRSRTGSVVYDGNETIDWPSERIARLGVAYVPEERGIFSSLNVLE
                     NLLLPPVVKPGGLSLDEIYELFPALRERSASQGTKLSGGEQQMLAIARILRTGADLLL
                     LDEPTEGLAPVIVQQIGRILRKLKEKGFTILLVEQNFHFAATVADRHYIVEHGQVVDM
                     LENVELTANKAKLESYLGV"
     gene            complement(3992..4792)
                     /locus_tag="VF226_19410"
     CDS             complement(3992..4792)
                     /locus_tag="VF226_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076211.1"
                     /translation="MTNDNIIETEGLSINFKGLWAVKDVNLNVRRGTIHALIGPNGAG
                     KTTCFNLITKFLTPTAGVIRYNGQDITRLQPADIARLGLVRSFQISATFPHLTLLENI
                     RVALQRKLGTSFHFWRSERSLDVLNDRALQLLDDVGLGAHAHERAVELPYGRKRALEI
                     ATTLALDPEMLLLDEPMAGMGREDIGRISELIRRVAKNRTVLMVEHNLSVVADLSDFI
                     TVLARGEILAEGPYEEVSRNPEVIEAYMGSGDGTGDSMRITANGAGHA"
     gene            complement(4943..5815)
                     /locus_tag="VF226_19415"
     CDS             complement(4943..5815)
                     /locus_tag="VF226_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7076212.1"
                     /translation="MTAAIWDFFALPLSFPFMQRALLVAVLVGAVAAVLSCYLVLKGW
                     SLMGDAISHAVLPGIVLAYVVGLPLSAGAFLAGLTCATLTGYLKENSRVREDTVMGIV
                     FSGMFGFGLVLFTKVETEQHLNHVLFGNLLGVTDSELTEIIVVAAVVLVAVLIRRRDL
                     LLYCFDQQHARAIGLRVGWLYYGLLALLSLAIVASLKAVGIILVVAMLVAPGATAYLV
                     SRRFERMLVIAILVAVFSSVAGTLLSFHLDAATGPSIVLLQAALFVLALIHTWVQRTR
                     TSAEHATTDQLATG"
     gene            complement(5812..6672)
                     /locus_tag="VF226_19420"
     CDS             complement(5812..6672)
                     /locus_tag="VF226_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7076213.1"
                     /translation="MSELLVPFEYNYMLRAMWVSALVGGLGAFLSAYLMLKGWSLMGD
                     ALSHAVVPGVAIAYLMQLPYAAGAFFAGIIAAFGMWGIRHVTRLKEDAVIGVVFTAFF
                     AAGLFIASLYPIPVSIQSIVLGNILGIADEDVVQVVVVAAVALAILLVTWKDLMLVFF
                     DESHARAVGLNPVALKVLFFALLSASAVTALQTVGAALVVATVITPGATAYLLTDRFG
                     RLLALSVAIGVSTSFVGAYVSFFLDGATGGVIVTLQTLVFLTAFVFAPKHGILAARRR
                     TRAAGEAPQP"
     gene            complement(6669..7592)
                     /locus_tag="VF226_19425"
     CDS             complement(6669..7592)
                     /locus_tag="VF226_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7076214.1"
                     /translation="MLARSQKDAEVGQAALEPSIDVNGLTVVYPNGHTALIDVSFSLG
                     PGTICGLAGPNGSGKSTLFKALMGFVRPARGEVRICGLPVSEALKRRMVAYVPQSEEV
                     DWNFPVLVEDVVMMGRYGHMNLLRIPRAADRREVDVALERVGMSELRNRQIGELSGGQ
                     RKRVFLARAIAQGAGIILLDEPFTGVDVKTETSIVALLRELREAGNLMLVSTHNLGSV
                     PDFCDQVVLLNRRLLAAGPTAETFTQANLERTFGGVLRHFRLGGPELHEDDDRRAVTV
                     LTDDERPLVFYGERGDESHVPSDQRLPRGKP"
     gene            complement(7598..8500)
                     /locus_tag="VF226_19430"
     CDS             complement(7598..8500)
                     /locus_tag="VF226_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7076215.1"
                     /translation="MASFLAVLALGLLPGGAAEAQGASRRAGKPLRVITTFTVIQDIA
                     QNVAGTAAVVESITKPGAEIHGYQPTPGDIVKAQQAELVLWNGLNLERWFERFFENIK
                     EVPSVVVSEGVEPMGITEGPYTGKPNPHAWMSPSNALIYVENIRKALAEHDPANAEVY
                     RRNAEAYAAKIKALDQPLRKRLSAIPEEKRWLVTSEGAFSYLARDYGLREAYLWPINA
                     DEQGTPQQVRRVIDLVRKYNIPVVFSESTISDKPARQVARETGARYGGVLYVDSLSEE
                     SGPVPTYLKLLEVTVDTIAKGFGL"
     gene            8735..10912
                     /locus_tag="VF226_19435"
     CDS             8735..10912
                     /locus_tag="VF226_19435"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7076216.1"
                     /translation="MKRSPAPEPSRLSAPRPTGANAPPDLDSDGRDKNDASRVDALIT
                     GALGLHAGDFVVHADHGIGRFEGLTRLDADGTRDFFTIAFQDGKVLVPVENADLLSRY
                     GSADSTVRLDRLGGKAWVSRKGRLKKRIGAIARRLIGIAAARELATAPVLAADPQDYA
                     RFCAGFPYQETPDQADAIAATIRDLVSGKPMDRLICGDVGSGKTEVALRAAFHAAMAG
                     WQVAVIAPTTLLSRQHTETFRKRFADFPVTIAQASRLTGSKRLAEVKAGLKSGEIRIV
                     IGTHALLSESVSFAKLGLIIVDEEQRFGVRHKERLKQLREGVHVLTLTATPIPRTLQL
                     ALSMVRSLSLIMTPPAGRRPVRTTVCEMEPARIGEALIAEKQRGGQSFYVCPRIRDLA
                     LVARMLEREVPEVSFVRAHGRMSAAELDRIMEQFLARHIDVLLSTTIIESGLDIPSVN
                     TMIVHDAHRLGLSQLYQLRGRIGRSGVEAIALLTVPPGAELTEAAKRRLDVLTRLEPV
                     GAGFSVASHDMDIRGAGNLLGEEQSGHIRDVGFDLFQTMLKRAVDALRKGEQPDLDIW
                     TPRISLGLPVLIPESYIPDPVERAEVYWKIAGIESEDELAAYARELRETRGPLPRLAQ
                     RMFILLRLKQLCREARVAELHAGPRGAILKLRPETPKPRNLPLPRGFAGRLTWRKGGE
                     LAVRAQWAEPRRRLKGLAILLSRLAHAISEPAQANVTPLRRAA"
     gene            complement(10920..13184)
                     /locus_tag="VF226_19440"
     CDS             complement(10920..13184)
                     /locus_tag="VF226_19440"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX7076217.1"
                     /translation="MSNIRVLPQPPASDEPKSAEPESTVEQESEPVSPHNQPEEPVGA
                     PLEEIRVDLLRHGERLAGLLADPGAAFVRDAMRVLEHQICRIAVIGQVKSRQSAFIGA
                     LIERPDLLPAEVSPWATAVVRLHFGSAAAPEHTEAEFQLFDKEDWQQLAAGADRIREL
                     TSQLVPGFEPELLRRHLEAMRQRAEQRFAGEFETLLGAKHAYETVSRDTLARYIGAGS
                     SAADASASPGQYADLTKSADIWLPSGPFRFPVTLIATPGINDPFMVRDEITRRTLEPA
                     ELYLFVVSARQPLSTAELANLRILRGLHRERILVFIDNIDELRDPAVEVRSIISDVRK
                     TLAREFPSVDIPVIAGSSKWALQALAPDKLRLSDGLVPSLLAYAKQKGALRPMEVKGG
                     GQAAPVEELPSGQLARILTICSGYAELRRELTGLLHRSHSAHLIRQIAAYFAELSQVN
                     EAAVREDMVKLAIVAENGNLGEAAKSDRQLKEVQAELARIQSVAGELERNLAGFQNLL
                     SEVVASHRTRLYEGLAALLEDYARGEANGLLQAFAEGQARRHWRCHTKPLRQRVEAEL
                     TAARRAVEEQLAATPHNVFPALRETMLRRSPDLGLPEAPHARLRPTSVRVPIEDSIAL
                     DLAPPGSIIGWQRSRTPEEHAAELERMLIEEFMPAIDEVIRLASAELQRETSDISDRA
                     TAISVVVIDALRNQSESYIARAKSLLDARETQTPSQEDHTQRLEELRERLTVAEALSR
                     RLADLNTRCGDVVT"
     gene            complement(13181..13393)
                     /locus_tag="VF226_19445"
     CDS             complement(13181..13393)
                     /locus_tag="VF226_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076218.1"
                     /translation="MSPDPLRSAGRHLLSEQRFVSTRITTPGATTLDLLNSSHLARGV
                     ITRPTTQIAVALRGAGLRQTREAEQK"
     gene            13754..14935
                     /gene="pobA"
                     /locus_tag="VF226_19450"
     CDS             13754..14935
                     /gene="pobA"
                     /locus_tag="VF226_19450"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012495335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEX7076219.1"
                     /translation="MRTQVGIIGAGPAGLLLARLLSRQGIESVVLERRSAAHVLGRVR
                     AGVLERGTVELLKEADCADRMQREGLVHHGLQINFDGRSLRIDLDGLTGNPVVVYGQT
                     EMTRDLMAAREAEGGTTIYEAEAVGCSHLETDKPQIIFRRNEQQEVLICDFIAGCDGF
                     HGISRTMIPASVLKTYARAYAFGWLGVLADVPPCSEELIYASHPDGFALASMRSPTRS
                     RYYIQCALDVRLEDWSDDRFWTELKRRLGPEAADRVTSAPSIEKSIAPLRSFVAEPMR
                     FGRLFLAGDAAHIVPPTGAKGLNLAAADVRVLARALTAYYKQSRTDLIDSYSAACLAR
                     VWKAERFSWWMTSLLHRFPDADSFTRKLQEAEFYYLRRSRAAQLSLAENYVGLAFDLQ
                     G"
     gene            15633..16673
                     /locus_tag="VF226_19455"
     CDS             15633..16673
                     /locus_tag="VF226_19455"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEX7076220.1"
                     /translation="MVGAPNAVCLCADKNMLVPALFVADSVRRAASSGVQNFDVMIFT
                     PPGDAADDFRQWAAGRGISLRDDLDLSQLQDVKILQRRLSRAALMRLLVPVSVAGQYR
                     RILHLDADLTIHDDVGVLFELDMADTAVAAVPAGRVPAAERLEVWDWWRSHFEALGMT
                     YPYRYFNAGVMLIDTEKWVRDDLTTRALEFIMQNGPICFYLEEDALNAVLDGAITELS
                     PIWNFRPKEQTLRRLIQPVIVHYAGRSKPWKRFGRSKRLFENLDAFRLYEAFIRESPW
                     PDWLATQWTVRDLVASLRAEAKYHLAAFSRSRANMDTLVETLFREYFEGAAFADVEQG
                     IAVRENGTLRLA"
     gene            complement(16685..17725)
                     /locus_tag="VF226_19460"
     CDS             complement(16685..17725)
                     /locus_tag="VF226_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076221.1"
                     /translation="MALVDSRAADISLYVEASRSGVLLEADCLVFLVGGFTFDSSPAA
                     NEQALILGQFYRDWGISAVPVVVRWGMELEAVDGARGQAGPNATLYVMPSGEGNGPDV
                     GRVPFVAPSKGIAIHLAGPHLFTSMSADRASIVLDLLLDMVADRDPTYAAALYGTCLE
                     TPLHGLSEYWKGILGSACYLSRCGGEALEIPDGATGISSYGDLRLLPATALADMGLRT
                     GGEPDVCPRWGLSIDDMRHIVRIHQLMAEAAAKAQLSQIRDTFHDTLSQLSELTGRYR
                     ELEEALTASKQRAAEFRVRERKSVLKRAKASLRKRLAAWRVPPRQAEGVLSVQQGMDP
                     AEWMRGDQVRRP"
     gene            complement(17712..20234)
                     /locus_tag="VF226_19465"
     CDS             complement(17712..20234)
                     /locus_tag="VF226_19465"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7076222.1"
                     /translation="MSELMHAIHADRVGTAQPRIAVIIPVYRQPQFLIESVYSVLNQT
                     LPGVVGVIVNDGCPYETSHRLASSLAALHPERIHYIYQRNRGLAAARNRGIRYSLARW
                     PEIAAIFPLDCDNVLDSEALEKLYAMLLANRDAGWVYPYLERFGASTAVWRNAPSPTI
                     TRMLFENQCDAGSLISRRVFESGIYYDETMREGYEDWEFFLRALEHGFRGVSAGAVGF
                     RYRTRRASMLTEAAAKHEHLVRQLHARHPNVIEPRRRTELENAELPRFVLLDIETGRK
                     LMFTDPEHACEHNGHRHLSRYVPPYSIIGSWSAFEAIKKMGLGPGLLMFVQHAAQRRN
                     VKIVLKGGELPIAFRTYHRKRDDTAFALFCGDTRRLVKKSGLRRFPVMVIEIASCNFS
                     KTCGRQFGISDVAETVSQFGIRELIEQEWRDENDSARLARQVIKQRPARVFMRDLHSD
                     LSRSTFPIGPSTGKDILIAAPWIKLGGVDQCIFKLSGAIRELEPTSRLHLLLSEQGEV
                     EIPSILNQGLFDTITSLGGLGRPSKAQIAASMMRSMDLVINAHSLAGYDAAASRCVSG
                     AASSLPPMISYLHVYDLDKGGYPVGYPFMAAGLEPFIAKYAVISEKMKYLLINSGVPP
                     EKVFLARNAPVVSPGLERGMELAAQKAARINSGEPLRLLYAGRLDYQKGGTRLSRIAH
                     LLSAAGLKFKLRVVGKATAGFPHEFVDLPPGIAEFHPPEYDYERLAECYSEADVLLLP
                     SRWEGVPLAMLDAMAHACIVIATDVGAIGEVLESGRNGYLIANGEDGAVAEMAARRII
                     EIASSGGKTRKLREAAVETAAAFSWQDTARQILMEGGVHGFS"
     gene            complement(20670..22124)
                     /locus_tag="VF226_19470"
     CDS             complement(20670..22124)
                     /locus_tag="VF226_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076223.1"
                     /translation="MDINSRRLVIFSGALAFVIISKILIFYPSLQTDDIARLFDDTPY
                     FYFQQSRFAEAALSAILAQSGNAMIDIYPGAAVLYFVLLCALVALVVDRLTPQDIHFV
                     MPLLATGLIVSSPFSTTLLMFRSAIPAQVLGLTGVILFIRPILYPTSPARDLIISAFG
                     VVLALSSYQPYISLLSVVLVFCLASGMSGGTVCGRLGLREGVMAFLLGAISYGTLMVL
                     VPRLLGTPPAAAMRGGLIGPEELIPRIRMLIDSLSIPYGADDPMTRGGYLWCVGLCLL
                     CAALVQAVQIRDQPISVVATRLLMGTGILLVCSVTAVAPLLLFNTVWIDSRTQFAGAF
                     AVAFAVLYWAAAAPRPMQMVLAALLTGITLFGSAASNRMVIDQITLNRLDIRTATEIA
                     RRADDEAGGRHAGQFVLHDPPYSYGIIERSSLRDRNKSALSAPWSAPAVFSLATGKLH
                     AVEISRDDGFCTAWPRWPAKDAVRILGNTAHVCF"
     gene            complement(22190..23182)
                     /locus_tag="VF226_19475"
     CDS             complement(22190..23182)
                     /locus_tag="VF226_19475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7076224.1"
                     /translation="MPQRKVQDAAITIVVPVKDEEEAIIPFIIALDRAVAGCPARFSF
                     LFIDDGSIDRTISMLRELRERDPRVGYLKLSRSFGKEAAMTAGLDAAKGDAVIVMDVD
                     LQDPPELIPEFVRLWSAGFDTVYGARSSRSADGFLKRNTAEWFYWLFNKTAETKIPPN
                     AGDFRLMDRCVVNALVRMRETNRFMKGMFAWPGFSSTCVEYVRPERFAGTTKFNFRKL
                     WRFAIDGIVSYSTVPLRIWTYIGALIALLSFMYLGKVVLAKMIWGTVVPGYSSLMTAI
                     LFFGGVQLISIGIMGEYLGRLYMETKRRPLYIIEECAADVASANTTDLMCADLV"
     gene            complement(23247..23489)
                     /locus_tag="VF226_19480"
     CDS             complement(23247..23489)
                     /locus_tag="VF226_19480"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7076225.1"
                     /translation="MTLLDEARMIVASALERPLEDVAPDDALESAPGWDSLGHMRIIL
                     GLEARLVRMLEAHEIVGIKSVSDIAALLQRQSTTGN"
     gene            complement(23486..24070)
                     /locus_tag="VF226_19485"
     CDS             complement(23486..24070)
                     /locus_tag="VF226_19485"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX7076226.1"
                     /translation="MTTEWKVGRPVRLETSRFQISSLTPLQALWHTYPWTFDSEVMHS
                     FGLPAGTWTRRSWYRRFRKSNNRRKFVLGIRAKHDNELIGLEIANVNSAGVAALSVLI
                     GHRDWWGKGVVQEVRSAVIDFLFDQVGCSRVWGTPSSRNFPSIFNYQKLGFTCEGILR
                     QHGLDPATKQHVDFVIFSMLRRDWKRKCGERVAA"
     gene            complement(24067..25527)
                     /locus_tag="VF226_19490"
     CDS             complement(24067..25527)
                     /locus_tag="VF226_19490"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX7076227.1"
                     /translation="MQLDLVPPTARIIDVRSGRALDYAAFRRAVGARAAVLREAVLRG
                     DTVAMCHRDGMVLLVDLFAVWQTGAAALVLSPSLTASERSRIAAWAKPACWIGQPVSP
                     EGIPRIAPELEDWADTQAGFCPSAQIGPSSNDAALILLTSGSTGMPKGVVLGHRALSI
                     RLSGNIRQIGKPTLARTLVPLPLHFGHGLIGNALSALFAGGDVLLWPEPGVNGLAHLG
                     AVLDEHAVTFMSSVPSMWRMALKLSPPPRQKMLKRVHVGSEPLPAKLWQDICDWAGTR
                     KVYNMYGMTEAANWISGASVEDAGFIEGAVGKPWTGEWRILREDGCLAESGHGEILLH
                     DPALMNGYANDPEATAAAFHGSWFRTGDIGELDSDGTLRIVGRRKNQINRGGIKIAAE
                     EVELILEQHPDVQQTCAFALPDVVSGEIVAAAVVLKNGSNTSQAALQAWCAERLRQEA
                     VPSRIIFLADIPRNDRGKKVRNAVREFAWAELGRSP"
     gene            25987..26268
                     /locus_tag="VF226_19495"
     CDS             25987..26268
                     /locus_tag="VF226_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX7076228.1"
                     /translation="MVQDSSVLALSENVSYQPLGEGEGAVVLIIDSGQLFTCNDTTSA
                     FLAAVDGQRSFGDVVRSLLSTFDVSHDELRNDLASLAEALQQEGIVRIQ"
     gene            26279..26728
                     /locus_tag="VF226_19500"
     CDS             26279..26728
                     /locus_tag="VF226_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HEX7076229.1"
                     /translation="MLRRIRFRIALWCWARLVPFLAWGGRKLSALLALTKPRETTPYR
                     GLAADYIVRRVKKATRRPWVMRDRPCLREGLLAVRFLRLAGYDPSLHFGVDRESVMRD
                     ALSAHCWVVLDEKTLLNPPSPGMVEILIYAGDRLMPSAPRSASATAA"
     gene            26739..27749
                     /locus_tag="VF226_19505"
     CDS             26739..27749
                     /locus_tag="VF226_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076230.1"
                     /translation="MISWFDLNGSLLRIDAADPELLRPLLIYLDELQTDPRGPAAFTF
                     IVERGSPMSEPASARLLFEGPLPEAPTSRLYAGVDNRSLIIPDRLSMEYSIPDRIARF
                     RVAAGDENLVGGTAAIYAIHAALFATGQTLVHAAALRLPDRNEALVLFAPSGAGKTTT
                     SLALALQGFGLLTDDATVLAAGSGSRGRTNVWGLPRPPKVHQRTAQMLPAIGGLLGSK
                     WTPEGEQSVSIEGLKSVIEVIPGRAYPLCALVLLGERVTGPHRLRPMRKADLFMHFAT
                     DNVTRSPEGVLHEELSRFQKFIGLVATTPAYQLNVGSDLSTLGEMVAAELGGAGQAIL
                     SA"
     gene            complement(27728..28771)
                     /locus_tag="VF226_19510"
     CDS             complement(27728..28771)
                     /locus_tag="VF226_19510"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7076231.1"
                     /translation="MSGPKPHIPLDRNGERLLIHIADPAQTVPTPPVPLPDAEGLSLL
                     LTAAEKHGVLPTAIRALGKLIEAPSDAPGAEASKAACEVLEQARLKLAYQTGFQILMS
                     HHARRIMAAFTAAGIQGAVVKGAAFAQRLYPDSALRTFTDVDVLICEPHRRKTLDVMP
                     ALGFELFEFEDRKGKDYHEQKWLLAGKQDLMIEVHSNLVHSPKLRGAMSIGYEDVLEA
                     GGGNCSDATALLFVAATHGAIGHQFDRLQHLVDVMQAARGAAGPVDLDRLARVSHRCG
                     VRLAIAGALELAGKTFNEPRCLMLAKQLVPSALSRLPGQLLSPELVVRGQSFNRARGS
                     WRRNLFRQALRIA"
     gene            complement(28898..29950)
                     /gene="speB"
                     /locus_tag="VF226_19515"
     CDS             complement(28898..29950)
                     /gene="speB"
                     /locus_tag="VF226_19515"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020049963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7076232.1"
                     /translation="MDHENLERLRAKYGGAAAGDVHDPEFKKVVDILFSGADRRAKPY
                     EGVSTFLDAPLALDAPEQADFGGLDIALIGVPMDLGVTNRPGARHGPRAVRQVERIGP
                     YHEFHRVVPASLAKIADVGDVPFRSRYSLADSHEDIAAFYEKVCRAGVAPVSVGGDHS
                     IVLPILKELGRDRPLGLIHIDAHCDTGGPFEGSKFTHAGPFREAVLAGVLDPQRTIQI
                     GIRGNAEYLWEFSYDSGMTVIHAHEFMQLGVESTIARMREVVGSGPVYISFDIDSIEP
                     GIAPGTGTPEVGGLTPREVMALIRGAAGLDIVGGDVVEVAPAYDPTTATAQIAAQMLF
                     EIVTVTAMARARSRAA"
     gene            complement(30070..>30427)
                     /locus_tag="VF226_19520"
     CDS             complement(30070..>30427)
                     /locus_tag="VF226_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7076233.1"
                     /translation="VLAILLTAYIVLGSVMDELAMILLTLPVFFPVVSALDFGLPAEE
                     VAIWFGILVLIVVGVGLVAPPIGLNVFVVSALAKDVSIISIYRRVLPFLAMDVVRLVL
                     VTAFPALALVLVRLLN"
BASE COUNT         5959 a         9669 c         9236 g         5563 t
ORIGIN      
        1 ggcccccgac ggtgcggcga gcaccgcgag ggcgagcgcc gcgatcagcc gcgccgccgc
       61 gcccaccatg ccgccagctc ccccacgatg ccgcgccgga acgcgagcac gcacacgacg
      121 aagatcgcgc cggtgatcac ggtcacccac tggccgcggt cggcgaggta gtgctcgagc
      181 gagatgatcg tgaacgcgcc gaccaccggc ccgaatacgg tgcccatccc gcccaggagc
      241 gtcatcagca cgacctcgcc cgacatctgc cacatgacgt ccgtcaggga ggcgagctgg
      301 aagacgatgg ccttcgtcgc gccggccaat ccggacaggg tcgccgacag cacgaaagca
      361 agcagcttgt actgatccac gtgatagccg agcgagacgg cacgctgctc attctcgcgg
      421 atagccttca gcacttgccc gaacggcgaa taaatcgcgc ggtaaatgat gaggaagcca
      481 gccgcgaaga tcgcgaggac gacgtaatag aaggtcaggt cgtcggtgat gtcgataacg
      541 ccgaagacag ggcgccgcgg taccgcctgg ataccgtcct caccgccagt gaacggcgcc
      601 tgcaggcaga agaaaaagac catctgcgcc agcgccagtg tcaccatggc gaagtagatg
      661 ccctgccggc gaatggcgat gaagcccgcc acgacaccca gcagcatcgc gacgccggtg
      721 cccgcgagaa ttcccagctc cgttggccag ccccaggcct tcaccgtgta ggcggagatg
      781 taggcggcga agccgaagaa catggcatgc ccgaacgaca gcaggcccac gtatccgatc
      841 agcaggttga aggcgagtgc gaagagggca aagcacagca ccttcatcag gaacaccgga
      901 tagagcaccg cgggcgcgat cagcagcccc gccagcagcc cgacgaatat ggtcatgtcg
      961 taatttttca tcgcaggctc ccgatcgcct gcccgcaagc aggaagggtg tgggtcgcgt
     1021 gtccgtgcat cgaaaccatc cctgccctcc tcaccgcggc gttccgaaca gcccggcggg
     1081 gcggagcatc aggacgatgg ccatgatgat gaaaatgaca gtgttggacg cttccggata
     1141 aaagaccttc gtcaacccct cgatcactcc gaggccgaag ccggtcagga tcgaccccat
     1201 gatggagccc atgccgccga tcaccacaac ggcgaacacg acgatgatga gattggcccc
     1261 catcacaggg ttaaccgaat agatcggtgc ggccagcaca ccagcgagtg cggcgagccc
     1321 tacgccaaag ccgtaagtca gcgtcatcat gcgtggcacg ttgatgccga aggcgcgcac
     1381 cagcgtcggg ttttccgtgg cggcgcgaag atagctgccg agcttggtct tctcgatcat
     1441 gaaccatgtg gcgaggcaca cgacgagcga cagcaccacg atccacccgc gataccaggg
     1501 caggaacatg aagccgagat tggtgccgcc gggaatggga ttcgtatagg gcagtccgga
     1561 cgacccgtac tgctgccgga acacgccttc gatgatcagc gccaggccga atgtcagcag
     1621 cagtccgtaa aggtggtcga ggtgatagag ccgtgacaga aagagccgtt cgagcagaat
     1681 gccgaatgcg ccgatgatga tcggagtcag gatcagcgcg ccccagtagc cgacgcccaa
     1741 gtactgaagc agcatgtagg ccgtgaacgc gcccatcatg tactgggccc cgtgggcgaa
     1801 attgatcacg ttcagcatgc cgaaaatgac agccaagccg aggctcagga gtgcatagaa
     1861 ggcaccgttg atgagcccca gcagaagctg gccgaaaagc gcctgcggcg gtatgccgat
     1921 gagctcgaac atggattgat ggctccgcgc cctacctcca tccgggagaa agggccaatg
     1981 cgcggggcga cgcgctctcg cgcgccgccc tcggtcagtc tcgatcagcg ataacccgtc
     2041 agctcttcac gagcgggcat ccgccctctg aaaggggccg gaaggcctcg ttggcgggga
     2101 tggtggcacg cagcttgtag tagtcccacg gccccttgct ctcctcgggt ttcttcacct
     2161 cgaagaggta catgtcgtgg atcttgcggc catcaggacg gatgatgccc ttgccgaaca
     2221 gacggtcctc ggtcggcatc tccttcatct tggcgaccac cttggcgcca tcggcatcgc
     2281 tgcccagcgc atgcaccgcc ttgagatagt gcgtcaccgc cgagtagacg ccggcctgca
     2341 ccatcgtcgg cttgtggccc ttgttgtcca cctcgaattc cttggcgaag gcgcggttgt
     2401 tgtcgttcag gtcccagtac caggcctcgg tgaagatcag gccctgggcg gtcttcaggc
     2461 cgagcgcatg cacgtccgtg atgaacatga gcagagccgc gaggttctgg ccgccctgcg
     2521 tgatgccgaa ctcggccgcc tgcttgacgg agttaatcgt atcgccgccg gcattggcga
     2581 ggccgatgat cttggcgccc gatgactgcg cctgcaggag gaaggacgag aagtcctggc
     2641 cggggaacgg atggcgcacc ttgccgagaa ccttgccgcc gttcgcttcg acgaccgccg
     2701 ccgtgtcacg ctcgagcgca tggccgaagg cgtagtccgc ggtcaggaag aaccaggtat
     2761 cgccgcccgt cttgacgacg gccttccccg tcccgttggc gagcgaccac gtatcatagg
     2821 tccagtggat cgtgttggga gagcagtcct tgccggtcag gtccgacgtg gcgccggtcg
     2881 agttgaggtg aaccttgttc ttctctctgg tgatattgtg gactgcgagc gccaccgaag
     2941 aggtcggcac gtcgacgatg acgtccactc cctccacgtc gtaccactgg cgagcgatgt
     3001 tggcgccgat atccggcttg ttctgatggt caccgaagat gacctcgatc ttgtcgtggc
     3061 acttgttggc cttgcagtaa tcctcgaccg ccttacgggc cgcccacacc gagccctggc
     3121 ccgagaggtc ggcgtacaca ccggactggt cgttgagcac gccaatcttg atgacgccgt
     3181 ccgtgaattg cgcgtgcgcc gctgtcgcga gcacgctggc agcagctgcc agaacgaagg
     3241 tcgttgctct cacactatcc tcctattgac gcttcctttg acgacttaga caccgaggta
     3301 gctttccagc tttgccttgt tcgcagtgag ctcgacgttc tcgagcatgt ccaccacctg
     3361 cccgtgctcg acgatgtaat gccggtcggc gacggtggcg gcaaagtgga agttctgctc
     3421 gacgagcaga atggtgaagc ccttctcctt gagcttccgg agaatgcggc cgatctgctg
     3481 gacaatgacc ggcgccagcc cttcggtcgg ctcgtcgagc agcagcaggt ccgcgccggt
     3541 gcgcagaata cgcgcgatcg ccagcatttg ctgctcgccg ccggagagct tcgtgccctg
     3601 gctggcgctg cgctcccgaa gcgccggaaa cagctcgtag atctcgtcca ggctgaggcc
     3661 gcccggcttg acgaccggcg gcaggagcag gttctcgagc acgttgaggc tcgaaaagat
     3721 gccccgctcc tctggaacgt aggcgacgcc caatcgtgcg atgcgctcgg agggccaatc
     3781 gatggtttcg ttgccgtcgt agacgaccga gccggtgcgg gatcgcacga tgcccatgat
     3841 ggcgcgcatg gtcgtcgttt tgccgacgcc gttgcggccg agcagcgtca cgagctctcc
     3901 cttggagaca tcgaaggtca tgccgtgcag gacgtggctt tcgccgtacc aggcgtggag
     3961 atcgcgcacg gacagaagcg gtgcggtcgg ctcaggcatg gcctgccccg tttgccgtga
     4021 tgcgcatgct gtcccctgtg ccatctcccg agcccatgta ggcctcgatc acctccggat
     4081 tgcgcgagac ctcctcatag gggccttcgg ccaggatctc gccgcgcgcc agaacggtga
     4141 tgaaatccga caggtccgcc accaccgaca ggttgtgctc gaccatcagc accgtcctgt
     4201 tcttggcgac gcgccggatc agttcggaga tgcggccgat gtcctcgcgg cccatgcccg
     4261 ccatcggctc gtcgagcagc agcatctcgg gatcgagcgc gagtgtcgtg gcgatctcga
     4321 gggcgcgctt gcgtccgtat ggcagctcga ccgcgcgctc atgtgcatgg gccccgagcc
     4381 ccacgtcatc gagcagttgc agggcgcggt cgttgagcac gtcgagcgat cgctccgagc
     4441 gccagaaatg aaaggaggtg ccgagcttgc gctgcaaggc aacgcggatg ttctcgagca
     4501 acgtcaggtg cggaaaggtg gccgagatct ggaagctgcg gacaagcccc aggcgcgcga
     4561 tgtccgccgg ttgcaggcgc gtgatatcct ggccgttata ccggatgacg ccggccgtcg
     4621 gggtcagaaa cttggttata agattgaagc acgtcgtctt gccggcaccg tttggcccga
     4681 tcaacgcgtg aatcgtgccg cggcgcacgt tcagattgac gtccttgacg gcccaaagcc
     4741 ccttgaaatt gatggacagg ccttcagtct cgataatgtt gtcgttggtc atacggggct
     4801 tcagccctgt ccttattctt tcccttcccc ggcctatagc acaggaccct tttgcgtcca
     4861 tactgcattg gcgcgctgaa ataaacaaca acccggcgtg cggcagaata agcctttggt
     4921 tctagtgtga gattgacttc ggtcagccgg tggcgagttg gtccgtggtg gcatgctcgg
     4981 cactggtccg cgtgcgctgc acccaggtgt ggatcagggc gaggacgaaa agtgccgcct
     5041 gcagcaggac gatgctgggg ccggttgcag catccaggtg gaagctcaga agcgttccgg
     5101 ccacgctgga gaagacggcg accaggatgg ctatcaccag catccgctcg aaccgccgcg
     5161 acacgaggta ggccgtggcg cccggcgcca ccagcatcgc caccaccagg atgatcccga
     5221 ccgccttcag cgaggcgacg atcgcgaggg acagcagtgc gagcaggccg tagtacagcc
     5281 atccgacccg caagccgatg gcacgggcgt gctgctggtc gaagcagtag agcagcagat
     5341 cccggcgccg gatcagcaca gcaaccagca ccaccgccgc aacgacgatg atttccgtca
     5401 gctcgctgtc ggtgaccccc agcaggttgc cgaagaggac gtggttcaga tgctgctcgg
     5461 tctcgacctt cgtgaagagc acgaggccga acccgaacat ccccgagaac acgatgccca
     5521 tgaccgtgtc ctcgcggacc ctgctgttct ctttgagata gcctgtcagc gtcgcgcagg
     5581 tgagccccgc aaggaaggca ccggccgaca gcggcaggcc cacgacgtag gcgagcacga
     5641 ttccgggcag cacggcgtgg gagatggcat cgcccatcag cgaccagccc ttgagcacga
     5701 gatagcacga cagcaccgcc gcgacggcac ccaccagcac cgccacgagc aacgcccgct
     5761 gcatgaacgg gaacgacaga ggcagggcga agaagtccca gatcgcagcg gtcatggctg
     5821 cggcgcctcc cctgctgcgc gggtccggcg ccgggccgcc aggatcccgt gtttcggtgc
     5881 gaagacgaat gccgtcagga agaccagcgt ctgcaaagtc acgatgaccc cgccggtcgc
     5941 gccgtcgagg aaaaagctga cgtatgcgcc gacgaagctg gtcgaaacac cgatcgccac
     6001 gctcagcgca aggaggcggc cgaagcggtc cgtcaggaga taggccgtgg cgcccggcgt
     6061 gatcacggtc gccaccacga gtgccgcgcc gaccgtctgc agcgcggtga cggcggaggc
     6121 gctgagcagc gcgaaaaaaa gcaccttgag cgcaaccggg ttcagcccca ccgcccgcgc
     6181 atggctctcg tcgaaaaaga cgagcatcag gtccttccag gtgacgagca gaatggcgag
     6241 cgcgactgca gccaccacga cgacctgcac cacgtcctca tcggcaatgc ccagaatgtt
     6301 gccgagcacg atgctctgga tgctgaccgg gatggggtag agcgaagcga tgaacagccc
     6361 tgcggcgaag aaggcggtga acaccacgcc gatcaccgcg tcctccttca gccgcgtcac
     6421 gtggcggatc ccccacatgc cgaacgcggc tatgatgccg gcgaagaacg ctcccgccgc
     6481 ataggggagt tgcatcaggt aggcaatcgc gacccccggg acgacggcgt gcgacagcgc
     6541 atcgcccatc agcgaccagc ccttgagcat caggtaggcc gacaggaatg cgccgaggcc
     6601 gcccacgagc gcactgaccc acatcgcgcg cagcatgtag ttgtattcga acgggacgag
     6661 gagctcgctc atggcttgcc gcgtggcaac cgctgatccg acggcacgtg gctttcgtcc
     6721 cctctctcgc cgtagaacac gagggggcgc tcgtcgtcgg tgagaacggt gacggctcgg
     6781 cggtcgtcat cttcgtgcag ctccggacct cccagacgga agtggcgcag cacgcccccg
     6841 aacgtgcgct cgagattggc ttgggtgaac gtctcggccg tcgggccggc ggccaatagc
     6901 ctgcgattga gcagcacaac ctgatcgcag aagtccggca cactgcccag gttatgggtc
     6961 gagaccagca tcagattgcc ggcctccctc aactcccgca acagcgcgac gatggacgtc
     7021 tccgtcttga catccactcc ggtgaacggc tcgtccaaca ggatgatccc ggcgccctgc
     7081 gcgatggcgc gggcgagaaa cacgcgcttg cgctggccgc cgctcagctc gccgatctgg
     7141 cggttgcgaa gctcgctcat gcccacccgc tccagcgcga cgtcgacctc ccggcggtcc
     7201 gcggcgcgcg ggatacggag caggttcatg tgaccgtagc gccccatcat caccacgtcc
     7261 tccacgagca cggggaagtt ccagtcgacc tcctcgctct gcgggacgta ggccaccatt
     7321 cggcgcttca aggcctccga caccggcaat ccgcagatgc gcacctcccc gcgggccggc
     7381 cgcacgaagc ccatgagcgc cttgaagagc gtggacttgc cgctgccgtt cggcccggcg
     7441 agcccgcaga tcgtccctgg gccgagcgag aagctgacgt cgatgagagc cgtgtggcca
     7501 ttggggtaga ccaccgtgag gccgttgaca tcgatgctgg gctccagcgc ggcctggcca
     7561 acttccgcgt ccttctggga ccgtgccagc atgagcatca gagtccgaag cctttcgcta
     7621 tggtgtcgac ggtgacttcg agcagcttga gataggtcgg cacgggcccg ctttcttcgc
     7681 tgagcgagtc gacgtacaga acgccgccat agcgcgcgcc agtctcccgc gcgacctgac
     7741 gcgcgggctt gtcggaaatg gtgctttcgc tgaacacgac cgggatattg tatttgcgca
     7801 cgaggtcgat cacgcggcgg acctgctgcg gcgtgccttg ctcgtcggca ttgatgggcc
     7861 acaggtaggc ttcgcgcagg ccgtagtcgc gtgcgagata gctgaacgca ccctcgctgg
     7921 tgaccagcca gcgcttctcc tcggggattg ccgaaagccg cttgcgcagc ggctggtcaa
     7981 gcgccttgat cttcgccgca taggcctcgg cgttcctgcg gtaaacctcg gcgttcgccg
     8041 gatcgtgctc cgcgagagcc ttgcggatgt tctcgacgta gatcaatgcg ttcgacgggg
     8101 acatccaggc gtgcgggttg ggcttgccgg tgtatggtcc ttccgtgatg cccatgggct
     8161 cgacgccctc gctgacgacc acgctcggca cttccttgat gttctcgaaa aagcgctcga
     8221 accaccgctc caggttcaag ccgttccaga gcacgagctc ggcttgctgc gctttcacga
     8281 tgtcgcccgg tgtcggttga tagccgtgaa tttccgcacc cggcttggtg atgctttcca
     8341 ccaccgccgc cgtgccggct acgttctggg cgatgtcctg aatgacggtg aacgtggtga
     8401 tgacgcgaag cggcttgccc gctcgccggc tggcgccttg cgcctcggca gcgcctcccg
     8461 gcagcaagcc taacgcgagc acagccagga acgaagccat gagatgccgg cgccctgtgt
     8521 tgacccgctt caacctcaat tcctcacgaa ggacgggcca caggcatcca ggaggcatac
     8581 gtttcggtct ccagttgcaa ctgatttgca attgcaagat cagattagga gcgttttgcg
     8641 tcttgtcaac ggcaggagtc cgttccccgt gcgatttaat gtgaggagaa catcggtagc
     8701 aggaacgcag gaaagcgggt ttcggttgag tggcatgaag cgatcgccgg caccggagcc
     8761 ctcgcgccta tcagccccga ggcctacggg tgcaaacgcg ccgccggacc tcgacagcga
     8821 tggacgtgat aagaacgacg cttctcgggt cgacgcgctg atcacgggcg cactcggcct
     8881 gcatgccggc gattttgtcg tccatgccga ccacggcatc ggccgtttcg aagggctcac
     8941 caggcttgac gcggatggca cgcgcgactt cttcaccatc gcctttcagg acggcaaggt
     9001 gctcgtcccg gtcgagaacg cggacctgct ctcccggtac ggttcggcag actcaaccgt
     9061 tcggctggat cggctcggcg gcaaggcatg ggtgagccgc aaggggcggc tcaagaagcg
     9121 catcggcgcg attgcccggc ggcttatcgg aatcgcggcg gcccgcgagc tggcgaccgc
     9181 gcccgttctc gccgccgacc ctcaagatta cgcccgcttc tgcgcaggct tcccgtacca
     9241 ggagacgccc gatcaagcgg acgcgatcgc ggctacgatc cgggacctcg tcagcggcaa
     9301 gccgatggac cggctgatct gcggggatgt cggctcaggc aagaccgagg tggcgctccg
     9361 cgccgccttc cacgcggcga tggccggctg gcaggtggcc gtgatcgcgc ccaccaccct
     9421 gctctcgcgc cagcacacgg aaactttccg caagcgcttc gccgatttcc cggtcaccat
     9481 tgcccaggcc tcgcgactga cgggttccaa acgcttggcc gaggtcaagg ccggattgaa
     9541 atccggcgag atccgcatcg tcatcggcac ccacgcactg ctttccgaga gcgtcagttt
     9601 cgccaagctc ggcctgatca ttgtcgatga ggagcagcgg ttcggtgtcc ggcacaagga
     9661 gcgcctgaag cagctgcgcg aaggggtcca cgtcttgacg ctcaccgcga cgccgatccc
     9721 tcgcaccctg cagcttgccc tgtcgatggt gcgctccctg tcactgatta tgactccgcc
     9781 cgccggccgg cgcccggtgc gcacgaccgt ctgcgaaatg gagccggcgc gcatcggcga
     9841 agcgctcatc gccgagaagc agcggggcgg ccagagcttc tacgtttgtc cacggatcag
     9901 agatttggcg ctcgtcgccc gcatgctcga gcgggaggtg cccgaggtga gcttcgtgcg
     9961 cgcccacggg cgcatgtcag ccgccgagct cgaccgcatc atggagcagt ttctggcccg
    10021 gcatatcgat gtgctcttgt cgacgacgat catcgagtcg ggcctcgaca tcccgtccgt
    10081 aaacacgatg atcgttcacg acgcacaccg gctcggtctt tcgcagctgt atcagctgcg
    10141 gggccggatc gggcgctcgg gcgtcgaggc catcgcgcta ttgactgtgc ccccgggtgc
    10201 cgagctcacg gaagctgcca agcggcgcct ggacgtgctc accaggctgg agcctgtggg
    10261 agccgggttc agcgtcgcaa gccacgacat ggacattcgc ggcgccggca acctgctggg
    10321 agaggagcag tccggtcaca tcagggacgt cggcttcgac ctgttccaga ccatgctgaa
    10381 gcgggcggtg gatgcgctcc gcaagggcga gcagccggac ctcgatatat ggacgccgcg
    10441 gatctcgctc ggcctgccgg tcctgatccc cgaaagctat atccccgatc cggtcgagcg
    10501 cgccgaggtc tactggaaga tcgccggcat cgagagcgag gacgagctcg cagcctatgc
    10561 ccgggagctt cgggagaccc gcggaccact gccgcgcctc gcgcagcgga tgttcatcct
    10621 gctgaggctg aagcagctct gccgcgaggc acgcgttgcc gagctgcacg ccggccccag
    10681 aggggcaatc ctcaagctga gacctgaaac gccgaagcca cgaaacctgc cactcccgcg
    10741 cggcttcgct ggtcgcctca cctggcgcaa ggggggcgag ctcgcggtac gcgcccaatg
    10801 ggcggagccc cggcgacgcc tgaaagggct ggcgatcctt ctcagccggc tcgcgcatgc
    10861 catctcggag ccggcgcaag cgaacgtcac gccactgcgc cgggctgcct gattggccat
    10921 caggtcacaa catctccgca gcgggtgttg aggtcggcaa ggcgccggct caaggcttcg
    10981 gcaacggtca atcgctcgcg cagctcttcc agacgttggg tgtggtcctc ctgacttggg
    11041 gtctgggtct cgcgggcatc gagcaacgac ttggcacgcg cgatgtagct ttcgctttgg
    11101 ttgcggaggg catcgatcac cacgaccgag atggctgtcg cacggtccga gatgtcactc
    11161 gtctcccgct gcagctcggc gcttgcgagg cggatcacct cgtcgatggc cggcatgaac
    11221 tcttcgatca gcatgcgctc cagctcggcc gcgtgctcct cgggcgtgcg gctgcgctgc
    11281 cagccgatga tcgacccggg cggggcgagg tcgagcgcga tcgaatcttc gatcggtacc
    11341 cggaccgagg tcggccgcag cctggcatgc ggcgcctcag gtaacccgag atccggggag
    11401 cgccgcaaca tcgtctcgcg gagcgccggg aagacgttgt gcggtgtcgc cgcgagctgc
    11461 tcctcgactg cacggcgggc ggcagtgagc tcggcctcga cccgctgacg caaaggcttg
    11521 gtgtggcagc gccaatgccg ccgcgcctgc ccctcggcaa atgcctgcag cagaccgttg
    11581 gcctcgccac gcgcatagtc ctcaagcagc gcggccagcc cctcgtacag ccgtgtgcgg
    11641 tggctagcga cgacctcgga aagcaggttc tgaaagcccg ccaggttccg ctccagctcg
    11701 ccggcgacgg attgaatacg cgccagctca gcctggactt ccttgagttg ccgatcgctc
    11761 ttggcagcct ctccaaggtt gccattctcg gctacgatgg cgagcttcac catgtcttcg
    11821 cggacggcag cctcgttcac ctgcgaaagc tcggcgaaat aggcggcaat ctgccggatg
    11881 agatgggcgc tgtggctgcg gtgcagaagc ccggtcagct cacgccgcag ctcggcatag
    11941 cctgagcaga tcgtcaggat gcgggcaagc tgtccactgg gcaactcctc gaccggagcc
    12001 gcctgcccgc cgcctttgac ctccatcggc cgcagcgctc ccttctgctt ggcataggcc
    12061 agcagcgacg ggaccaggcc gtcgctcagc cgcagcttgt ccggggcgag cgcctgaagg
    12121 gcccatttcg agctccccgc aatgaccggg atatcgacgg acggaaattc gcgcgcaagc
    12181 gtcttgcgca cgtctgagat tatgctccgc acctcgacag cgggatcgcg cagctcgtcg
    12241 atgttgtcga tgaacacgag aatgcgctcg cggtgcagcc cgcggaggat acgcaggttt
    12301 gcaagctccg ccgtcgacag cggctggcgc gcactgacga cgaagaggta gagctcggcc
    12361 ggctccaggg tgcgccgcgt gatctcgtcc cgaaccatga acggatcgtt gatgccgggc
    12421 gtcgcaatga gcgtcaccgg aaacctgaag gggccgctcg gcagccagat atcggcggat
    12481 ttcgtgagat cggcgtactg gccgggcgat gccgacgcgt cggcagcgct gctccccgct
    12541 ccgatatagc gggcgagcgt gtctcttgag accgtctcat aggcgtgctt cgccccgagc
    12601 aaggtttcga attcgccggc aaagcgctgc tcggcccgtt gccgcattgc ctcgagatgc
    12661 cgccgcagca gctccggctc aaaccctggc acgagctggg acgtcagctc acggatacgg
    12721 tcggcgccgg cggcaagctg ctgccagtcc tccttgtcga aaagctggaa ctcggcttcg
    12781 gtgtgctcag gggcggctgc ggagccgaag tgcagccgaa ccacggccgt cgcccagggg
    12841 ctgacctcgg ccggcagcag gtccggacgc tcgatcagcg caccaatgaa cgcgctctgg
    12901 cgcgacttca cctggccgat aaccgcgatc cggcagatct gatgctccaa aacccgcatg
    12961 gcatcgcgca cgaacgcggc accgggatcg gcgagcagtc cggcaagccg ctcgccatgg
    13021 cgcaggagat cgaccctgat ctcctcgagc ggcgccccga ccggctcctc gggctggttg
    13081 tggggcgaaa caggctcgga ctcctgctcc acggtggact ctggttccgc cgatttgggc
    13141 tcatcggacg ctggtggctg aggcagtacc cggatgttgc tcatttctgc tccgcctctc
    13201 ttgtctggcg cagtccggcg cctcgcaacg cgaccgcgat ttgcgtcgtc gggcgagtga
    13261 tgacgcctcg cgcaagatgc gaactgttca gaaggtcgag cgtggtggcg ccaggtgtgg
    13321 tgatccgcgt cgagacgaac cgctgctctg aaagcagatg ccgaccggcc gatctcagag
    13381 gatccggact catacgaacg ccacccccgg acgaaacttt cgtttgtcgg tcaaaggaaa
    13441 attgccgact gtcccgcgct gggaccggtt acgcccaagg ttgttaatga actgttaatt
    13501 gtggcgtggg catggagaat gattgcggtt gcggggcacc cgcgcgctcg aacgtctgtt
    13561 gacgggatat tctcgccaga atatggcctc cccgcgcgcg caccggccat ggcgagagcc
    13621 tgggccaatt cgctgagctc aggaagggag cacggccgct ctcctcaccc tcacagcagc
    13681 gaacagcccc ttttcccctg cccgaaaaag cgtcactctg ccggaaaaca ttaacctgat
    13741 ctcgagggca ggagtgcgca cccaggtagg aatcatcggc gccgggccgg ccggattgct
    13801 gctggcacgg ctgctctcac ggcagggcat cgaaagcgtc gtcctcgaac ggcgcagcgc
    13861 ggcacacgtg ctcgggcgcg ttcgtgccgg cgtgctggag cgcggcacgg tcgagctgct
    13921 gaaggaagcc gattgcgccg accgcatgca gcgtgagggc ctcgtccacc acggacttca
    13981 gatcaacttc gacggtcggt ctctccggat cgatctcgac ggcctcaccg gcaacccggt
    14041 cgtggtgtac ggccagacgg agatgacgcg cgacctgatg gcggcacgtg aggcggaagg
    14101 cggaacaacg atctacgaag ccgaggccgt gggttgcagc catctcgaaa ccgacaaacc
    14161 acagatcatt ttccgtcgaa acgaacagca ggaggtgctg atctgcgatt tcatcgccgg
    14221 ctgcgacggc ttccacggca tcagccgcac gatgatcccc gcctctgtcc tcaaaaccta
    14281 cgcgcgagcc tacgccttcg gctggctggg ggtgctggcc gatgttccgc cgtgctcgga
    14341 ggagctgatc tacgcgagtc accccgacgg cttcgcgctc gccagcatgc gctcgcccac
    14401 gcgcagccgc tattacatcc agtgcgcgct cgacgtgcgt ctcgaggact ggtccgacga
    14461 ccgcttctgg acggagctca agcgccggct cggtcccgaa gctgccgatc gcgtcacctc
    14521 agcaccctcg atcgagaaga gcattgcgcc gctgcgcagc ttcgtggccg agcccatgcg
    14581 tttcggccgc ctgttccttg ccggcgatgc cgcccacatc gtgccgccga cgggagccaa
    14641 gggtctgaac cttgccgctg ccgacgtgcg cgtcctcgcc cgcgcgttga ctgcctacta
    14701 caagcagagc cgaacggatc tgatcgactc ctactccgcc gcgtgcctcg cccgcgtctg
    14761 gaaggcggaa cggttttcgt ggtggatgac gtcgctgctg caccgcttcc cggacgcgga
    14821 cagcttcacc cgcaagctgc aagaggcgga gttctactat ctccggagat cgcgagcggc
    14881 ccaactctca ctggcggaga actacgtggg cttggcgttc gatctgcagg gttaataacc
    14941 ctgcaggacg cgaactaaaa aataagcgcc cccacggctc atacaagaga aggcgccaag
    15001 cgtcgattga gccgctgttg caaagacagc gttattctgg agttcagtgc gaacttacct
    15061 cagatagccg tcgaagaacg ggctgacaaa acggccgtca gccgtgatgg tggcaagctg
    15121 ctcccgcttg gtgagaacgg gcttctcgta attacgcttc attcgtgcct cctgatttcc
    15181 ggttgaagca attgcactca tatccgagtc tcctcattgg ttgaagaggc gctctattgc
    15241 aatccttggt tgtttttgag ggttggtgtt ggtttcggcg tttctgtagc aagagggtca
    15301 cacatcgaaa ccggggccgc cctgcacgat gcgtttgcta cgacatacgc tggctgcgtg
    15361 acatggctcg gccgaaaacg ccaccacaag aatccccgcg acagacatca cttcgtgccg
    15421 tagatgcgca ttctgctcca gggcacgcga tctgttgggg caaggctcaa cgccccgctg
    15481 tggacaccag cgagctccgc agcagcgatg catcgacagg cgactcaatc cgcactagta
    15541 gattcgttta cacactactt aacattgatc caccagcccc gactttctgc tggtgtcctg
    15601 tgcttgtccc acttggccag tgagcgaaca tcatggttgg tgcacccaac gcggtctgtc
    15661 tctgtgccga taagaatatg ctcgtccccg ccttgttcgt cgcggatagc gtcaggaggg
    15721 cagccagctc cggcgtgcag aacttcgatg tcatgatatt cacgcctccg ggggacgccg
    15781 ccgacgattt ccggcagtgg gctgcgggac gaggaataag tctccgggac gatctcgatc
    15841 tttcgcaatt gcaggatgtc aaaatcttgc agcggcgcct ttcacgagcc gccctcatgc
    15901 gccttctcgt gccagtgtct gtagctggcc aatacagacg gatcctacac cttgatgccg
    15961 atctgacgat ccacgacgat gtgggcgtcc tcttcgagct cgatatggcc gatacggccg
    16021 tggcggctgt gccggcaggc cgggtgcctg ctgcggaacg cctggaggtt tgggattggt
    16081 ggcggtcaca tttcgaagcg ctgggaatga cctacccgta ccgctacttc aatgcgggcg
    16141 tgatgttgat cgacacggaa aaatgggttc gcgacgatct tacgacccgc gcgctcgagt
    16201 tcatcatgca gaacgggcca atctgctttt atcttgagga ggacgccctc aatgccgtcc
    16261 tcgacggcgc aatcaccgaa ctctcgccca tctggaattt ccgaccgaaa gaacagactt
    16321 tgaggagatt gatccagccg gtgatcgtgc attatgccgg acggtcgaaa ccctggaagc
    16381 gcttcggcag gtcgaagcga ttgttcgaaa acctcgacgc cttccgactc tacgaggctt
    16441 tcatccgaga aagcccttgg cccgattggc tcgccacaca atggactgtc cgagacctcg
    16501 tggcaagtct tcgtgcggaa gcgaaatatc atctcgccgc attctcacgc agccgcgcga
    16561 atatggacac ccttgtcgag actttgttcc gagaatactt cgaaggtgca gcattcgcgg
    16621 atgtcgaaca agggatcgca gtgagggaga acggaaccct gaggcttgca taatgcggtc
    16681 tcgctcacgg ccgtcgcacc tgatcgcccc tcatccattc cgcaggatcc atcccctgtt
    16741 ggactgagag tactccttct gcttgcctcg gcgggacacg ccaggccgcg agacgcttac
    16801 ggagcgacgc tttagctctc ttcagaacag atttccgttc tcgtactcga aactcggcag
    16861 cacgttgctt cgaagcggta agggcttcct cgagctcgcg atatcggcct gtcagctcgc
    16921 tcaactggga caacgtatcg tggaatgtat ccctgatctg agaaagctgg gctttcgctg
    16981 ccgcctcagc catcaattga tggatgcgca caatatggcg catatcgtcg atggacaatc
    17041 cccagcgggg gcaaacgtct ggctcccctc ccgtccgcaa ccccatgtcc gccagggccg
    17101 ttgcaggaag gagccgcaga tcgccatacg atgaaattcc ggtcgcgccg tccggaattt
    17161 caagcgcttc cccgccgcac cggctcaaat agcaagcaga tccgagaatt cccttccaat
    17221 actccgaaag accgtgaagc ggcgtttcca agcacgttcc atacagggca gcagcatagg
    17281 ttgggtctct gtccgcgacc atatcgagaa gaaggtccaa tactatcgaa gcgcggtctg
    17341 cagacattga ggtgaagaga tgaggccctg ccaagtgaat ggctattccc ttcgaaggcg
    17401 cgacgaatgg aacacgtccg acgtcagggc cattaccctc ccctgagggc atgacgtaca
    17461 gcgtagcatt tggaccagct tgacctcggg cgccatcgac ggcttccagc tccattcccc
    17521 acctcaccac gacaggcacg gcgctgatgc cccagtcacg ataaaattga ccgaggatca
    17581 gcgcctgctc attcgctgct ggactggagt cgaaggtgaa gcctccgacg agaaacacca
    17641 agcaatcggc ctcgagcaac actccagatc gagacgcctc gacatacaac gagatgtctg
    17701 ccgcccgact gtcaactaaa gccatggacg ccaccttcca tgagaatctg tctcgccgtg
    17761 tcctgccagc tgaaagccgc ggccgtttca acggcagctt ctcgaagctt gcgcgtcttc
    17821 ccgccggagc ttgcaatctc gatgattctt cgagcagcca tttccgctac ggctccgtct
    17881 tcgccattcg caatgagata gccgttgcgg ccgctctcca gcacctcgcc gatagccccg
    17941 acgtcggtgg cgatcacgat gcaggcgtgg gccatggcgt cgagcatcgc cagcggtacc
    18001 ccctcccatc gcgaaggaag gagcaagaca tccgcttcgg agtaacattc cgcgagccgc
    18061 tcgtagtcat actcgggcgg atgaaactcc gcgatacccg gtggcagatc gacgaattcg
    18121 tgaggaaagc cggctgtggc ttttccgacg acccttagct taaatttcaa ccccgccgcc
    18181 gacagaagat gcgctatccg tgacagcctg gtaccccctt tttggtaatc caagcggcct
    18241 gcatagagga gccgcagcgg ctcgccgctg ttgatgcgcg ccgctttctg tgctgccaat
    18301 tccatcccgc gctcgagacc tgggctcacc accggtgcat tgcgcgccag aaaaaccttc
    18361 tcaggtggaa cgccagagtt gatcagcaga tatttcatct tttccgatat gacggcgtac
    18421 ttggctatga acggctcgag accagctgcc atgaacggat acccgaccgg atagccaccc
    18481 ttatcgaggt catacacgtg caggtaagaa atcatcggcg gcaatgatga cgcggcgccg
    18541 ctcacgcatc tcgaggctgc agcatcgtag cctgccaggg aatgcgcgtt tatcaccaga
    18601 tccatgcttc gcatcatcga cgccgcgatc tgagccttgc ttggccgtcc cagccccccg
    18661 agcgacgtta tggtatcgaa caatccctga ttgagtatac ttgggatttc cacctcaccc
    18721 tgctcgctga gcaggagatg taaccgactc gtaggctcca gctctcggat ggcgccactc
    18781 aatttgaaga tgcattgatc cacgcctccc agcttgatcc acggggccgc tatcagaatg
    18841 tctttcccgg tggatggacc aattggaaag gtggaccttg acagatcaga gtggagatcc
    18901 cgcatgaaaa ccctggccgg acgctgcttg atgacctgcc gggccaagcg cgcggaatcg
    18961 ttttcatctc gccactcctg ttctatgagc tcgcgtattc caaactggga cacagtctct
    19021 gccacgtcgc tgattccgaa ttgtcggccg caagttttgc tgaaattgca tgacgcgatc
    19081 tcgatgacca taacgggaaa ccgcctgagg ccgctcttct tcacgagacg gcgggtgtcg
    19141 ccacaaaaca aggcgaacgc tgtgtcgtcg cgcttcctat gatatgttcg gaacgctatc
    19201 ggtagctcgc ctcccttgag gacgatcttt acgttgcggc gctgcgcagc gtgctggacg
    19261 aacatgagca gacccggacc caatcccatt tttttgatcg cctcgaaggc agaccatgag
    19321 ccaataatcg aatatggagg tacgtaccga gacagatgac gatgcccatt atgctcgcaa
    19381 gcatgttccg ggtccgtgaa catcagcttg cgccctgttt cgatatccag aagcacgaag
    19441 cggggtaatt ccgcattctc cagttctgtt ctccggcgcg gctcgatgac gtttggatga
    19501 cgcgcatgca gctgccggac aaggtgttca tgcttcgccg ccgcttcggt aagcatggat
    19561 gcccggcgag tccgatatcg aaatccgaca gccccggccg atacgcctcg gaacccgtgt
    19621 tcgagggctc ggaggaaaaa ctcccagtcc tcataaccct ccctcatggt ctcatcataa
    19681 tagatgccgc tctcgaacac gcgccttgag atcaagctgc ccgcatcaca ctggttctca
    19741 aacagcattc tcgtgatcgt aggcgacgga gcattgcgcc atacggccgt cgacgctcca
    19801 aaccgctcca aatagggata aacccagccc gcatcgcggt tcgcgagaag catcgcgtag
    19861 agtttctcca gtgcctcgga atcgagaaca ttatcgcagt cgagggggaa aatcgctgcg
    19921 atttccggcc atctggcaag agaataccga atgccgcggt ttctagcagc ggccagcccg
    19981 cgattgcgct gataaatgta atgaattcgc tccggatgca gcgctgccag cgagcttgcc
    20041 agtcgatggc tggtctcata agggcaaccg tcgttgacga tgacccctac cacgcctggt
    20101 aaggtctgat ttagcactga ataaacggat tcgatgagga actgtggttg acgatagacg
    20161 ggaatgatca cagcaattcg cggttgcgcc gtgcctactc gatcggcgtg gatcgcatgc
    20221 atcaactcac tcatggcgtc gcgatttttc tacgagctga actgtattac cggctttctc
    20281 gaattgcagt tgaccaaggc gtccggacga ctgcgggcgg tagggggcaa gattttccgc
    20341 ccccgcatga gaatggccac cgcataagtt cgagttcaaa gccaacctcc actgcgggcg
    20401 cggggacagc caccgggcgc gcaagcgccc agcggctctg gcatcagttt cttagtcact
    20461 ctacttctgg ttgtagtctc tcattcatgc tctggccgcg tcaacgcttc ggcgccgggg
    20521 gagcgctcca cgtcccccac tatcaacaac tttgacgact gctggcgaaa tcagagggat
    20581 cggctgatgt gcggtcgggt caaaaatgcg gacccggcca ataatgcttt cctgcgtccg
    20641 cggcgcttca aagttcgcct gctcccgcct cagaagcaga catgcgctgt attgcccaat
    20701 attctcaccg catcctttgc gggccaccgg ggccaggccg tgcagaagcc gtcatcgcgg
    20761 ctgatctcca ccgcgtggag cttgcctgta gctaagctga acactgcagg cgcggaccat
    20821 ggggcgctga gtgctgattt gttcctgtcc cttaaggacg aacgctcgat gatgccataa
    20881 ctataaggcg gatcatgtag gacgaactgc cctgcatggc gccctcctgc ctcatcgtcg
    20941 gcacggcgag caatttcagt ggcggtcctg atgtcgagtc gatttagagt aatctgatcg
    21001 atgaccattc ggttgctggc agcagaacca aacagagtaa tcccagtcaa gagtgcggcc
    21061 agcaccatct gcatcggcct cggcgccgct gcggcccagt aaagcactgc gaaggcgacg
    21121 gcgaaggctc cagcgaactg ggtccgggaa tcaatccaaa ccgtattgaa cagtagcagc
    21181 ggagcgacgg cggtgacaga gcagaccagc agaatacctg ttcccattag aaggcgggtc
    21241 gccacaacgg atattggctg gtcccgaatt tggactgcct gcacgagtgc agcacagagc
    21301 agacaaaggc cgacgcacca cagatagccg ccccgggtca ttggatcatc ggcgccgtaa
    21361 gggatcgaca ggctgtcgat cagcatgcga atgcgaggaa tcaattcctc tgggccgata
    21421 aggccgcccc gcatggcggc agcaggcgga gtccctaaca gtcgcggaac caaaaccatt
    21481 aacgttccgt aagagattgc acccagcagg aaggccatta cgccctcccg tagacccaac
    21541 cgcccgcaaa cggtcccgcc agacatcccc gaggcgaggc agaaaaccaa taccaccgat
    21601 aacagcgaga tatagggctg atacgacgac agcgcgagca caacgccgaa agcagatata
    21661 attagatccc gcgccggtga ggtggggtag agaatcgggc ggatgaacag gatcactcca
    21721 gtcaacccga gtacctgcgc aggaatggcc gaacggaaca tcagtaatgt cgtgctgaaa
    21781 ggcgaggaga cgatcagccc tgtagcgagc aacggcatta cgaagtgaat atcttgcggg
    21841 gttaagcggt cgacaacgag cgcgacaagt gcgcagagca gcacgaagta cagcaccgca
    21901 gccccgggat agatgtcgat catcgcgttg ccggattgcg ctagaatcgc cgaaagcgcc
    21961 gcttcggcaa aacgactttg ctgaaaatag aaatacggcg tatcgtcaaa aagtcttgcg
    22021 atgtcgtccg tctgcaacga ggggtagaaa attaaaatct tagatataat gacaaaagca
    22081 agtgcccccg agaagatcac aaggcgtcgt gaattgatgt ccaatttagg catcttaaaa
    22141 gaactttgtg caaagattgg ctatccagtc gccaccatcc atcttacact taaactaggt
    22201 cagcacacat taagtcagtc gtattcgccg acgcaacatc agcggcgcat tcctcgatga
    22261 tgtataaggg gcggcgcttg gtctccatat aaaggcgacc aaggtactcc cccataattc
    22321 cgatggagat gagctgcacg cccccgaaaa acaggatcgc cgtcatcagc gacgaatacc
    22381 ctggcactac agtgccccag atcatcttcg ccaggacgac cttgccaaga tacataaacg
    22441 acagcagggc gatcaacgca ccgatgtagg tccagatccg cagcggcacg gtgctataac
    22501 ttacgattcc atctattgca aatcgccata gcttgcggaa gttgaacttc gttgtgcctg
    22561 cgaaccgctc cggacgcaca tactctacgc aggtggacga aaatccgggc caggcgaaca
    22621 tccctttcat gaaacgattg gtttcccgca tcctgaccag tgcgttcacc acgcagcgat
    22681 ccatgagacg gaaatcgcca gcgttcggcg gaatcttcgt ttcagccgtt ttgttgaaaa
    22741 gccaatagaa ccactctgca gtatttcgtt tcagaaatcc gtccgcggag cggctcgacc
    22801 gcgcgccgta aacggtatcg aagccggcgg accacaaacg aacgaactca ggaatgagct
    22861 cgggtgggtc ctgcagatcg acgtccatga cgattacggc atcgcccttt gcagcatcga
    22921 ggcccgccgt catcgccgcc tcttttccga aactccgact gagcttcagg tatcccactc
    22981 gcgggtcccg ctcccgcaat tcacgaagca tggagatagt acggtcaatt gatccgtcat
    23041 caatgaagag aaatgaaaaa cgcgctgggc atcccgccac ggccctgtca agtgcaataa
    23101 taaatgggat gatcgcctct tcctcgtcct tcaccggtac gacgattgtt atggctgcat
    23161 cctgcacctt gcgttgaggc atccctcttc tccagcctgg caagtagcca gatttcccgc
    23221 cgcatcctac agaagcttac atcggctcaa ttgccggtcg tactctggcg ctgcagcagc
    23281 gctgcgatgt ctgaaacgct cttgattccg acaatctcat gcgcctccag catacggacg
    23341 agcctcgcct cgaggccgag aatgatgcgc atgtgtccga ggctgtccca gcccggcgcc
    23401 gactccaatg catcgtcggg cgcgacatcc tcgaggggcc gctcgagcgc gcttgcaacg
    23461 atcatccgtg cttcatcgag cagtgtcatg ccgcaaccct ttcaccgcac ttccttttcc
    23521 aatcacgacg cagcatggag aaaatcacga aatccacgtg ctgtttcgtg gcgggatcca
    23581 ggccatgctg gcgaaggatt ccctcacagg tgaatcccaa cttttgataa ttgaaaatcg
    23641 acggaaaatt gcgagacgag ggcgtccccc atacgcgcga gcatccgacc tgatcgaaaa
    23701 ggaaatcgat cacggcgctg cgcacctcct ggaccacacc cttgccccac cagtccctgt
    23761 ggccgatcaa cacggagagc gccgccaccc cggccgaatt gacgttagca atctcgagcc
    23821 cgatcagctc gttgtcgtgt tttgctctga tccccaggac gaatttgcgc cgattgttcg
    23881 acttgcggaa tcggcggtac caggagcgcc tcgtccatgt ccccgccggc agaccaaacg
    23941 aatgcatgac ctcagaatcg aaggtccaag ggtatgtgtg ccaaagtgcc tggagcggcg
    24001 tcaggctcga aatctggaat ctggatgttt ccagccgtac cggccggccg actttccatt
    24061 cggtggtcat ggactccgcc ccagctcggc ccaggcgaac tcgcgaaccg cgtttcgaac
    24121 cttcttgccg cgatcgttgc gaggtatatc ggccaggaaa atgatgcggg acggcaccgc
    24181 ctcctgccgc aggcgctccg cgcaccaggc ctgcagcgcc gcctgacttg tattgctccc
    24241 gttcttcagc acgacggcgg cagccacgat ctctccggaa acaacatcgg gcagcgcgaa
    24301 agcacatgtc tgctgcacat ccgggtgctg ctcgagaatg agttcgactt cctccgctgc
    24361 aattttgatc ccaccacgat tgatttgatt ttttcgtcta cccacaattc gcagggttcc
    24421 gtccgaatcc aactcgccga tatctccggt acgaaaccag ctcccgtgga acgccgcggc
    24481 cgtggcctcg ggatcgttgg cgtagccatt catcaacgcg ggatcatgga gaagaatttc
    24541 gccgtggccg ctttccgcaa gacagccgtc ctcgcgcagg atgcgccatt cccccgtcca
    24601 cggctttccg acagcgccct caatgaaccc cgcgtcttcc acgctggcgc cggaaatcca
    24661 attcgccgcc tctgtcatgc cgtacatgtt atagactttt cgggtgccgg cccaatcgca
    24721 gatatcttgc cacagcttcg ccggcagcgg ttcggagcca acgtgcacgc gcttcagcat
    24781 tttctggcgc ggcggcggcg acaatttcaa agccatgcgc cacatggacg gaacggagct
    24841 catgaatgtg actgcatgct catccagcac cgcgccaaga tgcgccaggc cgttcacacc
    24901 gggctccggc cagaggagga catcaccgcc cgcgaaaaga gctgatagcg catttcctat
    24961 cagtccgtgc ccaaaatgca gtggcagcgg aacaagtgtg cgagcgagcg tgggctttcc
    25021 gatttggcga atgttgccac tcagtcgaat agaaagagcg cggtggccga gtacgacgcc
    25081 tttgggcatc cccgtgctgc cggacgtgag caggatcagc gccgcatcgt tactcgaggg
    25141 gccgatctga gcggagggac aaaaaccagc ctgtgtgtcg gcccaatctt ctaactcggg
    25201 tgcgatgcgg ggaattcctt cgggagaaac cggttgtcca atccagcagg ctggcttggc
    25261 ccaggcggcg atacggctgc gctcggaggc cgtcagagat ggcgacagca cgagtgcggc
    25321 ggctccggtc tgccatactg cgaacaggtc caccagcaga accatgccgt cgcgatggca
    25381 catcgcgacg gtgtcgcctc tgagaacggc ttcgcgcaac actgcggcgc gcgcgcctac
    25441 cgccctgcgg aaagccgcgt aatcgagcgc gcgccccgag cgtacatcga tgatacgcgc
    25501 ggtcggagga acgaggtcga gttgcatatc gtgaccggtg cgtggcgcgc aacgagacaa
    25561 cttttagttg cgcatcactt ctgacagcat caacccggca gatcagtcaa cggtgtgtga
    25621 tggcaaggcc tcaagaagct gtggatttgc aaaacctgga gggcgactgc ttcggcgctc
    25681 gatagcctcg gacaccgacc gacctcccgg acgagcattg ctccgactga cgcgaaatct
    25741 actcagccac aaggcctccg cagcttgagg aaccctcgtt cgaccgctcg tttcggagcc
    25801 ttcagtcagg ccggttcctc agtgctccgc gaacgctcag tcagctcttc tgagggaatt
    25861 ggagtggcct gcagtccaca tgttctgcac aacggaaacc ctggcccatc caaccaccgt
    25921 agcaatctct ggttgttgtc gatattgttc cacaccggat tcgggcaaca ttgaaggagc
    25981 gttgctgtgg ttcaggattc cagcgtgctg gcactctcag agaatgtttc ataccagcct
    26041 ctcggcgagg gagaaggcgc agtcgttttg atcatcgact ccgggcagct cttcacttgc
    26101 aacgatacga catccgcctt cctcgccgct gtggacggac aacgcagctt cggcgacgtc
    26161 gtgcgcagcc ttctcagcac attcgacgta tcgcatgacg aattgcgcaa cgacctcgca
    26221 tccctcgcgg aagcattgca acaggaaggc atcgttcgca ttcagtgagg gccgatcggt
    26281 gctccggcga attcggttca ggattgcgct ctggtgctgg gcgcggctgg ttccctttct
    26341 agcctggggg ggccgtaaac tgtcagcgct cctcgcgctg accaagccgc gtgaaacgac
    26401 gccttatcga gggcttgcgg cggactacat cgtccgccgc gtcaagaagg cgacccgtcg
    26461 gccgtgggtc atgcgtgatc gcccctgcct gcgcgagggc ctgctggccg tgcgctttct
    26521 gcgcctcgcc ggatacgatc cctcacttca tttcggcgtc gatcgcgaga gcgtgatgcg
    26581 cgatgcgctg tccgcccact gctgggtggt gctggacgag aaaaccctgc tcaatccgcc
    26641 gagccccggc atggtcgaaa tcctcatcta cgccggcgac cgcctgatgc cctccgcgcc
    26701 gcgctctgct tccgcaactg ctgcttgatg gatctcggat gataagctgg ttcgatctca
    26761 acggctccct tctgcgcatc gatgcggcgg atccggagct cttgcggccc cttctgattt
    26821 atctcgacga gctccaaacc gacccgcgag gccctgcggc gttcacgttc atcgtggaac
    26881 gtggatcgcc aatgtccgag cctgcatctg cccggctcct gttcgaaggt ccgctgcctg
    26941 aagcgccaac ctcgcggcta tatgccggcg tcgacaaccg gagcctgatc atccccgacc
    27001 ggctctccat ggaatattcc attccagacc gcatcgcacg gttccgcgtt gcggccggcg
    27061 atgaaaacct cgttggcggg acggctgcga tctacgcgat ccatgccgca ctttttgcta
    27121 ccgggcaaac acttgtgcac gcggctgctc ttcgcctgcc cgaccgcaac gaggcgctcg
    27181 tgctgttcgc acccagcggc gccgggaaga cgacgacatc gcttgcattg gccctacagg
    27241 gcttcggact cctgacggac gatgcgacgg ttctcgctgc cggtagcgga tcgaggggtc
    27301 gaacgaacgt ctggggcctt ccccggccac ccaaggttca tcagcgcacg gcgcagatgc
    27361 tgccggccat cggcgggctc ttgggctcca aatggacacc ggagggtgag cagagcgtca
    27421 gcatcgaagg gctgaagtca gtgatagaag tcattcccgg gcgcgcctac ccactttgcg
    27481 cgctggttct gctgggtgag cgcgtcacag gtccgcatcg cctcaggccc atgcgcaagg
    27541 ccgacctgtt catgcatttc gcaaccgaca atgtcacccg ctcgcccgag ggtgtgctgc
    27601 acgaggagct ttcgcgtttt cagaagttca ttggcctcgt cgccaccacc cccgcttatc
    27661 aactcaacgt cggcagcgac ctttccaccc tgggcgagat ggtcgctgcc gagctcggtg
    27721 gcgccggtca ggcgatcctc agcgcctgac ggaacaggtt tctgcgccag gagccgcggg
    27781 cgcgattgaa actctgtcca cggacgacga gctccggtga caggagttga ccggggaggc
    27841 gggaaagagc cgagggcacg agctgctttg ccaacataag gcagcgcggc tcgttgaagg
    27901 tcttgccggc aagctccaat gcgcccgcga tggcgagcct gacgccacag cgatgcgaaa
    27961 cgcgcgccag ccggtccaga tcgacagggc cggcagcacc tcgcgccgcc tgcatgacat
    28021 cgacgagatg ctgcagccga tcgaactggt gtccgatggc accgtgggtc gcagccacga
    28081 acagcagcgc cgtcgcgtcg gagcagttgc cgccgccggc ttcgagcacg tcctcgtagc
    28141 cgatcgacat cgcaccgcga agcttcggtg aatgaacgag gttgctgtgg acctcgatca
    28201 tcaggtcctg cttgccggcg agcagccatt tctgctcgtg gtagtccttg cccttgcggt
    28261 cctcgaactc gaacagctcg aagcccaacg ccggcatgac atcgagggtc ttgcgccggt
    28321 gcggttcgca gatcaggacg tcgacgtccg tgaaggtcct gagcgccgag tcagggtaga
    28381 gccgctgggc aaaagccgcc cccttgacga cggcgccctg aatgccggca gccgtgaatg
    28441 ccgccatgat tcgacgcgcg tgatgcgaca tcaggatctg gaagccggtc tgataagcga
    28501 gcttgaggcg ggcctgctcc agcacctcgc aagctgcctt gctcgcctct gcgcccggcg
    28561 catccgacgg agcctcaatg agcttgccca acgcgcggat ggccgtgggg agaacgccgt
    28621 gcttttcggc agccgtcagc agaaggctca gcccttcggc atcaggcaac gggaccggcg
    28681 gcgtcggtac agtctgggcg ggatcggcaa tgtggatgag aagccgttca ccgttccggt
    28741 cgagtgggat atggggcttt ggtcctgaca tgatctcgtt tccctccttg ccccctgccg
    28801 gaagctacag gaacgaatcc atgctgacca actttcccac acgttctgcg cgaagaatct
    28861 gttgattgcg cgctatagga gagcatccgc gctcctccta agctgcccgt gatcgggccc
    28921 tcgccatcgc cgtcacggtg acgatctcga acagcatctg agcggcgatc tgcgccgttg
    28981 ccgtggtcgg atcgtaggcg ggcgccacct cgacaacgtc acccccgacg atgtcgagac
    29041 cggcagcgcc gcggatcagg gccatgacct cccgtggcgt cagtccaccg acctcgggcg
    29101 taccggtccc gggcgcgata ccaggctcga tgctatcgat gtcgaatgag atgtagacgg
    29161 gtccgctgcc cacgacctca cgcatgcgcg caatggtcga ttcgacgccg agctgcatga
    29221 actcgtgggc gtggatcacc gtcatgccgc tgtcgtagga aaactcccaa agatattcgg
    29281 cattgccgcg gatcccgatc tggatcgtgc gctgcggatc gagcacgcct gccagcacgg
    29341 cctcgcggaa tggtccggcg tgggtgaatt tcgagccttc gaacggcccg cccgtgtcgc
    29401 aatgggcgtc aatgtggatg aggccgagcg ggcggtcgcg cccgagctct ttgagtatcg
    29461 gcaaaacgat cgagtggtcg ccgccgaccg acaccggagc cacgccggcc ctgcaaacct
    29521 tctcgtagaa cgcggcgatg tcctcgtggc tgtcagcgag gctgtagcgg ctgcggaacg
    29581 gcacgtcgcc gacatcggcg atcttcgcga ggctagccgg cacaacccgg tggaactcgt
    29641 ggtagggccc gatccgctcg acctgacgca ccgcccgtgg accgtgccgg gcgcccggcc
    29701 gattggtgac gccgaggtcc atcggcaccc cgatcagcgc gatatcgagc ccgccgaaat
    29761 ccgcctgctc gggagcgtcg agcgcgagcg gcgcatcgag gaaggtggag acgccctcgt
    29821 agggcttcgc gcgccggtcg gcaccggaaa agagaatgtc gacgaccttc ttgaactcgg
    29881 ggtcgtgcac gtcaccagcg gccgcaccgc cgtacttggc acgcagtctt tcgaggtttt
    29941 cgtggtccat gactttcaat cccgattgcc cgatcctggg gctgccctgg atcagcgaac
    30001 gcttgagtcg ataattcaag cccatcccga gatatttggc ctgtcatcca cgccaacaag
    30061 ccagtccgct cagttgagca ggcgcacgag gacgagtgcg agcgcaggga atgccgtgac
    30121 caggacgagg cgcacgacgt ccattgccag gaagggcagc acccgccggt aaatcgatat
    30181 gatggagacg tccttcgcca gcgcgctcac cacgaacacg ttgaggccga tcggcggcgc
    30241 gactagtccg acgcccacca cgatcagcac gaggatgccg aaccagatcg ccacctcttc
    30301 tgcgggcagc ccgaaatcga gcgccgacac caccgggaag aacaccggaa gggtgagcag
    30361 aatcatggcg agctcgtcca tcaccgagcc caacacgata taggcggtga gcaggatggc
    30421 gaggaca
//
LOCUS       DASVDU010000292        20951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_514823, whole genome shotgun sequence.
ACCESSION   DASVDU010000292 DASVDU010000000
VERSION     DASVDU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 20951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20951
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514823"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /gene="aroC"
                     /locus_tag="VF226_19525"
     CDS             <1..565
                     /gene="aroC"
                     /locus_tag="VF226_19525"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX7076234.1"
                     /translation="SNPFFCPDHGVVQTWASYLDQIRKSGSSVGAVIEVVAEGVPPGL
                     GAPIYGKLDQDLASALMSINAVKGVEIGAGMAAAQLTGEENADEMRIGPDGRPVFSSN
                     HAGGILGGISTGQPIVCRFAVKPTSSILRPRKTIDRFGNETEIMTKGRHDPCVGIRAV
                     PVGEAMVAIVLADHALRHRAQTGKWGL"
     gene            567..1286
                     /locus_tag="VF226_19530"
     CDS             567..1286
                     /locus_tag="VF226_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX7076235.1"
                     /translation="MGDVSQVKALVFDVFGTVVDWRTSIIRELEAFGARKGISADWAA
                     FTDEWRGLYQPSMEEVRSGRRPWTILDVLHRESLEKLLTKFGITELDEAEKDHLNKVW
                     HRLAPWPDSVPGLQRLKSRYVIGTLSNGNVGLLTWMAKNAGLPWDVILGAEVARAYKP
                     LPQAYLRTAEILGLRPEQMMLVAAHNDDLAAAAALGYATAFVARPTEYGPHQVKDFTA
                     DRAWDVVADSFLGLADALGCP"
     gene            complement(1294..1758)
                     /locus_tag="VF226_19535"
     CDS             complement(1294..1758)
                     /locus_tag="VF226_19535"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX7076236.1"
                     /translation="MRLALYQPDIPQNTGTILRLSACMNVAVDVIGPAGFDLSDRMLR
                     RAALDYLHYVDLTRHVSWEEYVASRRKATSPGRLILLSARGEHPYTKFRFASDDTLLL
                     GRESAGVPESVRALADASVRIPMRTGLRSLNVAVSAAMVLGEALRQTQLFPE"
     gene            2069..2605
                     /gene="petA"
                     /locus_tag="VF226_19540"
     CDS             2069..2605
                     /gene="petA"
                     /locus_tag="VF226_19540"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX7076237.1"
                     /translation="MSSHAEEPTRRDFLTIAGSAFAAVGGAMALWPFIAQMNPDASTQ
                     ALASVEVDLTPVKEGQAITVMWRGKPVFIRNRTPEEIQKAVATPVNDLPDPEPDNARV
                     KKGHENWLVLVGICTHLGCIPKGQSLNDVKGDYDGWFCPCHGSHYDTSGRIRKGPAPR
                     NLEVPTYEFVSDTKIRIG"
     gene            2620..3903
                     /locus_tag="VF226_19545"
     CDS             2620..3903
                     /locus_tag="VF226_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7076238.1"
                     /translation="MTTHQSTYEPKSSAEKWLDERLPIMRMMHAQFVDYPTPRNLNYL
                     WTFGGILTVVLGLQILTGVVLAMHYIPSATEAFDSVERIRRDVNYGWLIRNLHAVGAS
                     MFFLAVYVHLFRGLYYGSYKAPREVLWIIGVIIYLVMMATAFLGYTLPWGQMSFWGAT
                     VITNFFSAIPLVGESIVQWLWGGYAVSGVTLNRFYSLHYLLPFVLAGLVVLHIWALHV
                     AGQNNPTGIEPQTKSETVPMSPYAVAKDFVGMAVFAILFAWFVFFVPDYLGHADNYNQ
                     ANPLVTPPHIVPEWYFLPFYAILRAIPSKLGGVVAMFGAIAILAFIPWLDTSRVRSTK
                     YRPLYRWFFWLFVFTCLALGYLGSKPAEGTYVTWARVFTFYYFAHFLIVMPIIGVIET
                     PSKMPRSISEAVLGKKGNGVSAVSPASATAAAEKR"
     gene            3929..4789
                     /locus_tag="VF226_19550"
     CDS             3929..4789
                     /locus_tag="VF226_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEX7076239.1"
                     /translation="MTTMRKSLQVLMAAGLLAITAQATPAAEAVQIERQSWPFSGFFG
                     TFDKAQLQRGFQVYTEVCASCHELRRISYRNLAQPGGPEFPEDAVKALAAAAEVEDGP
                     DDTGAMFKRPGLLSDPLPKLYANEKEARSIHNGAYPPDLSLMARARNTENVAPWYIHP
                     FLMLRDIAVGYQEGGADYIYSLLTGYEDPPAGVQVMEGMNYNVAFPGHQIAMANPFAG
                     GDGQVEYQDGTPSTVDNYARDVAAFLSWTADPRLEERKRIGWVVILYLIVTAGLLYVG
                     KKRLWSRLEH"
     gene            4913..5794
                     /locus_tag="VF226_19555"
     CDS             4913..5794
                     /locus_tag="VF226_19555"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEX7076240.1"
                     /translation="MTRAILGIIGGSGLYHLPNIKNPHWEKVSSPWGEPSDEILFAEV
                     DGLPIRFLPRHGRGHPRAPSGINYRANIDALKRTGVTDLVSVSACGSLREDLPPGKFV
                     LVDQFIDRTFAREKSFFGNGLVAHVSMADPVSPGLVDEIESALKAEQIDYRRGGTYLV
                     MEGPQFSTRAESNLYRSWGCDVIGMTNMPEAKLAREAEMCYATVAMVTDYDCWHDGHE
                     AVDVAAVLEVMKANTETAQRMLARLTASFPREHKPCPIGSDRALEVAVITAPEARDPE
                     LVAKLDAVAGRILKPQA"
     gene            complement(5804..6151)
                     /locus_tag="VF226_19560"
     CDS             complement(5804..6151)
                     /locus_tag="VF226_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7076241.1"
                     /translation="MSDAVYPPSTPQLVQKRRQLAPDTEAAFRAFSQQVFSDGALPAK
                     MKQIIAVAVAHVTQCPYCIKGHTKAALRHGATEQEIMEAIWVASEMRAGGAYAHSLVA
                     LAEMEKVAEGKSE"
     gene            complement(6300..6686)
                     /locus_tag="VF226_19565"
     CDS             complement(6300..6686)
                     /locus_tag="VF226_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076242.1"
                     /translation="MAISDPPFSIPVADGGPDDLPRTLRREREARERQARERQAREMR
                     ERAAAMAYHGFGPHVSEGTSAPPGTITRLDLPFLHLTAFFLKAVFAAVPALIVLGLLL
                     WGVGHVLLNWFPDLVKMQILILFPPG"
     gene            6818..7363
                     /locus_tag="VF226_19570"
     CDS             6818..7363
                     /locus_tag="VF226_19570"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7076243.1"
                     /translation="MEEVTVDELKQLIRTIPDYPKPGIMFRDVTTLMRDADGFERAII
                     ALAKPFEAQKVDAVAGIEARGFILGGAVADRLGCGFIPIRKKGKLPWKTIGQEYTLEY
                     GVDAIEIHEDAIRQGERILIIDDLIATGGTADAAAKLVRRSGGEVVGAAFVIDLPELG
                     GLSKLEASGVKCHALVAFEGH"
     gene            7364..7609
                     /locus_tag="VF226_19575"
     CDS             7364..7609
                     /locus_tag="VF226_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076244.1"
                     /translation="MSEMNGNSLPPGRIREALAAVNAVRLKPDSAVPCPVCGNPGFSV
                     EDRSARPHAEWYALSCTSCGLHDNLHIPMGSTPGSFS"
     gene            7816..8913
                     /gene="mtnA"
                     /locus_tag="VF226_19580"
     CDS             7816..8913
                     /gene="mtnA"
                     /locus_tag="VF226_19580"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7076245.1"
                     /translation="MKIGGRHYRTIWLNPDGWSVEIIDQTKLPHRFETVTLTSCEDAA
                     RAILTMQVRGAPLIGVTAAYGLCLALREDASDEAMERAIEHLARQRPTAINLRWALEQ
                     VRAAVRNLPREKRVEAAYKCAAALADEDVETCRRIGVNGLALIKDIAARKKPGESVNV
                     LTHCNAGWLACVDWGTATSPIYQAHDAGIPVHVWVDETRPRNQGASLTAYELGSHGVP
                     HAIIVDNAGGHLMQSGKVDICIVGTDRVTANGDVANKIGTYLKALAAKDNGVPFYVAL
                     PHSTIDWTLDDGLSIPIEERSEDEVLKMPGRLPDGSVVTVEIAAPGSPAGNPAFDVTP
                     ARLVTGFITERGVAPASREGLLSLYPERKDA"
     gene            8996..9346
                     /locus_tag="VF226_19585"
     CDS             8996..9346
                     /locus_tag="VF226_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HEX7076246.1"
                     /translation="MLSRVGLAAALLLSLLVPAAAHHGWGSYDASKLVTVEGRILTSQ
                     YANPHVTLTVQGKDKTWTLTLAPVSRMRSRGAEQELLAPGKTITAVGYVSTRDPDEMR
                     AERITVDGKTIEMR"
     gene            9347..9838
                     /locus_tag="VF226_19590"
     CDS             9347..9838
                     /locus_tag="VF226_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEX7076247.1"
                     /translation="MLPEAPAIFIALEQSGVGAAIRQSVWIYPIANVTHILALTVLAA
                     SVAVMDLRLLGAFRETVPAAVIVPARRISMLALTIMVLTGLVLFTAEASHVVMNPVFQ
                     TKMGLIALAILNALIVQRTLPAVLIKTPAMTPLPPRFRVAAVTSLALWFCIAAAGRLI
                     AYF"
     gene            complement(9909..10952)
                     /locus_tag="VF226_19595"
     CDS             complement(9909..10952)
                     /locus_tag="VF226_19595"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7076248.1"
                     /translation="MNLPIPLTVLTGYLGSGKTTLLKAMLKRPELGGTAVIVNEFGEV
                     GLDDALIEAVQEDTVLLPSGCVCCAVRGDLINALVKLHDDMMRGAIPDLTRVVLETTG
                     LADPAPIAQTLVNDRDLFRIYRLDGIITTVDAQHGLDQLDRTFESARQIAMADRVVLT
                     KTDLVDADGITQLTAQVRVLNPAAPVLQAVKGAIEPSQIFDLNAYEIRDGTDVSRWLA
                     ADRYATHDEGCDHPHDNHDHDHDHDHGHLHGIRSFCLTFNEPLEASKLEALIELLRAT
                     QGSKLLRIKGLLNVREADQPFVIHGVQHVFYPVEMLERWPTDDRRSRIVFITRDLEEA
                     AVREHFAPLNPAA"
     gene            complement(11013..11582)
                     /locus_tag="VF226_19600"
     CDS             complement(11013..11582)
                     /locus_tag="VF226_19600"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX7076249.1"
                     /translation="MLRYLVAYVSREGQTEKVAHFVARRLEDDGALVRLVDIKAHETE
                     AGVDDCDAMVIAGSVHRGQFDGELSSFIMRHGEALRRCPSAFLPVSLSAASHEESERS
                     AIDEIAQRFVAEHGWMPDIVCHVAGAVHDRRLNPFERLILHAIVDSKGVERHPSGDTE
                     LTDWGSLKRFVREFEALVGSKVQAHPNET"
     gene            complement(11648..12136)
                     /locus_tag="VF226_19605"
     CDS             complement(11648..12136)
                     /locus_tag="VF226_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7076250.1"
                     /translation="MSGYFEDIEIGATVHLGSYTFTREAIIDFARKYDPQPFHLDEEA
                     ARHTLFGGLCASGWHTSAIWLRHLIDHRNREADLMRFQGQRPPRYGPSPGFEKLRWLK
                     PVYVGDTLTFTTRAKEKIDSRSRPDVGLVLSDNEAVNQNNELVFSVVSKVFVERRRPF
                     EG"
     gene            complement(12133..12618)
                     /locus_tag="VF226_19610"
     CDS             complement(12133..12618)
                     /locus_tag="VF226_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7076251.1"
                     /translation="MSRGDPTTAGSLHWEDVIVGQTVAFGRKRVTKDEIIGFARAFDP
                     QPFHIDEAAAEASMIGRLCASGWHSCAMLMRMLVDDVLANAAGLGSPGLDEVKWLKPV
                     FPGDELTARYTCTSKRVLKSRPGVGICQMLFEMLNQNGEVVMTWDVAQLFRLRAPEVA
                     Q"
     gene            12916..13638
                     /locus_tag="VF226_19615"
     CDS             12916..13638
                     /locus_tag="VF226_19615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076252.1"
                     /translation="MAAITPTYAFPKLSNPTSIPTEQPNDQAEPETKNEAKETQSAID
                     EYLHKLMMAESAGKAHAKNPRSSALGPYQFINSTFIEVVDRHFSDEVEGMSQSEILAL
                     RTDMEFARRAAIAFNNDNAKHLRENGVKPTFAHLRLSHLLGPNAAVKVLRASPSTKLT
                     KLLPSAVIKANPFMRGMTVANLVRWSEGEVGVGVWATPTEIAKSGESKAPAATEVKTE
                     TVPPLPEKKVEADPVEGTNRTL"
     gene            complement(13766..14863)
                     /gene="ychF"
                     /locus_tag="VF226_19620"
     CDS             complement(13766..14863)
                     /gene="ychF"
                     /locus_tag="VF226_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX7076253.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAEAANYPFCTIEPNVGE
                     VGVPDPRLDTLARIGKSAQKVPTRLTFVDIAGLVRGASKGEGLGNQFLAHIREVDAVA
                     YVLRCFEDPDVTHVEGRIDPLADAEVVETELMLADLESLERRRIPLEKKAKSGEKEAK
                     AQLAIIDKALALLREGKPARLAALTPEEEPLYRQLQLLTAKPVLYVCNVDEASAATGN
                     AYSRQVEERARQECAGCVIISAKIEAELASLSPEDREVYLAELGLKEPGLNRLIREGY
                     RLLGLVTYFTVGPKEARAWTVPEGTKAPQAAGVIHTDFEKGFIRAETIAYDDYVAYGG
                     EVGAREAGKMRLEGKDYVVKDGDVLHFRFAT"
     gene            15088..15702
                     /locus_tag="VF226_19625"
     CDS             15088..15702
                     /locus_tag="VF226_19625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX7076254.1"
                     /translation="MDLSSLLIFAGALLVAAASPGPGVAAIVARVLGRGMQGAFAFTA
                     GMALGDLIWLSCAVLGLAAIAQTFGGVFLVIKYLGAAYLLYLAWKLWTGPADALEVKV
                     DRSDAKPLRLFLGGLAVTMGNPKVMIFYLALLPTIIEMARVSWLGWLELSIVTLAVLA
                     IVFSSYVLLAVRTRKLIANTRAMRLLNRFTGAVMASAAAAVASR"
     gene            complement(15862..16026)
                     /locus_tag="VF226_19630"
     CDS             complement(15862..16026)
                     /locus_tag="VF226_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076255.1"
                     /translation="MSARSLRNTLARKKQASRKIARPKVTRIQGNTAGVRKRLKKEAG
                     KARSASAPAS"
     gene            complement(16106..16636)
                     /locus_tag="VF226_19635"
     CDS             complement(16106..16636)
                     /locus_tag="VF226_19635"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7076256.1"
                     /translation="MLSFFAAGGWTEYLLTFASEHARFIALLVFVLGFTESIVLISLF
                     VPSSVLFLGIGAIHSAAGGEFWPIWVAGATGAWAGDCVSYALGRYFKDDAGKVWPMRK
                     FPGFIPKGQMFCERWGVLGIVLGKFIGGLRPFVPVVAGMLSMPWALFLPSSGLSCLIW
                     AGAFLAPGYGISFIVN"
     gene            complement(16748..17272)
                     /locus_tag="VF226_19640"
     CDS             complement(16748..17272)
                     /locus_tag="VF226_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7076257.1"
                     /translation="MDLTQYVEPTVAFIRANQIWAGPIVFLLAFGESVAFLSLVLPST
                     VFLVAIGGLLGASGIPFWPVWLAAGIGGSLGYAISYWIGLYFKDSIHKVWPFKRYPQM
                     IPKGQRFFDRYGVFGVFLGHFFGPIRAVIPVVAGMYAMRQLPFQIANVSSAFLWATGV
                     LAPSTFGARWLMGA"
     gene            complement(17362..19185)
                     /locus_tag="VF226_19645"
     CDS             complement(17362..19185)
                     /locus_tag="VF226_19645"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7076258.1"
                     /translation="MTAPIRRPAAKQDWVALATRAFRSGDLKGARAAYIEALRHDRRN
                     PQLHFEQAAVLAALGEIEESVVHLTQALRFKPQHEEAAKRLSRLLARFAITDASRLEP
                     FGLKAALGISGVDTQPIAEAALKYLVAVTPELARALEAVSDGHADETARKLILNRTAD
                     VLRSDLLLSALQAGVVMEAGLERLLTALRRILLLEVPAERFEDRTLCGFALGLLAQAW
                     NNDHVWAESQAETEALARMEIDTAALLKGDLEMARRLLLVALYRPLETILSSLNAEDY
                     GDVKPKPLREVVQKELERRAEEKHAAERIPRLAPLTDETSRRVARQYESSPYPRWQSL
                     HVSQPGAMKATLSRYFEPQRLAFMDGEFDVLIAGAGTGKQALQSAFAYGPKARLLATD
                     LSAPSLGYAHCAAKRYGVDNIEFMVADILDLDRLDRQFDIIECVGVLHHMADPWAGWR
                     KLVSRLKPSGLMYVGLYSATARANFGILREQPDYPGPDCDDATARAWRAQLLDRPAGS
                     PGTELVVSRDFYTLHEFRDLALHASEQHVTLDEISRFLDENGLAFRGFTLDPLVLAAF
                     ARKYPESQWPGSLADWQAFERDRPQVFEAMYRFWCDRAPAG"
     gene            complement(19220..19666)
                     /locus_tag="VF226_19650"
     CDS             complement(19220..19666)
                     /locus_tag="VF226_19650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076259.1"
                     /translation="MIRRRAARRATSRLTCALMGMAAAACSAGFNSASQVGMGCVDDS
                     SHCIEQRRIALNAIMSDRSRAWVREPPTSEAYASGVRLFAFKTRKAELSCDELAIGRR
                     EAENAPAVLRGPGASHLTPAQVSRGLMLGAEVSRELAAEMKRRCRA"
     gene            19820..20791
                     /locus_tag="VF226_19655"
     CDS             19820..20791
                     /locus_tag="VF226_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX7076260.1"
                     /translation="MVHAIRIHQHGGPEVMTWEEIEVGEPGEGQVRLKHRAIGLNYID
                     TYHRSGLYKISLPAVIGTEGAGEVTAVGPGVTDLKVGDRVAYASPMGAYTEERLIPAD
                     RVVKIPDGIDDQTAAAMMLQGMTVRYLLKETYKVGPQTTMLLHAAAGGIGLIACQWAK
                     HLGATIIGTAGSPEKCKLAQDHGATYTINYRTEDFVARTKELTNGKGVDVVYDSVGKD
                     TFPGSLDCLKPRGLWVSFGNASGAVPPFDILMLSAKGSLYATRPTLATYTATREDLVA
                     TANDLFDVVSRGIVKINVNQTYALKDAAQAHRDLESRKTTGSTILLP"
     gene            20824..>20951
                     /locus_tag="VF226_19660"
     CDS             20824..>20951
                     /locus_tag="VF226_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX7076261.1"
                     /translation="MLKIYGRTNSINVRKVLWTAEEIGLTYTREDWGRGFRSTSEP"
BASE COUNT         3882 a         6767 c         6577 g         3725 t
ORIGIN      
        1 cagcaatccg ttcttctgcc cggaccatgg cgtggtgcaa acgtgggcgt cctatctcga
       61 tcagatccgg aaatcgggat cctccgtggg cgcagtcatc gaggtggttg ccgaaggggt
      121 gccgccgggc ctcggcgcgc cgatctacgg caagctcgac caggacctcg cctcggcgct
      181 gatgagcatc aatgcggtga agggagtgga aatcggtgcg ggcatggctg cggcccaatt
      241 gaccggtgag gagaacgccg atgaaatgcg catcggcccc gacggccgac cggtattctc
      301 gtcgaaccac gccggcggca ttctcggcgg catctcgacg ggtcagccga tcgtctgccg
      361 cttcgcggtc aagcccacgt cctcgattct cagaccccgc aaaaccatcg accgcttcgg
      421 caacgagacc gagatcatga ccaagggccg gcacgacccg tgcgtcggca tccgtgccgt
      481 gccggtgggc gaggccatgg tggccatcgt gctggccgac cacgcgttgc ggcaccgggc
      541 gcaaacagga aagtgggggc tctgagatgg gcgatgtctc gcaggtgaag gcgctcgtgt
      601 tcgacgtatt cggcaccgtg gtcgattggc gaacgagcat catccgtgag ctcgaagcct
      661 tcggcgccag gaagggcatc tccgccgact gggccgcctt caccgatgag tggcgtgggc
      721 tttatcagcc ctcgatggag gaggtgcgct ccggacgccg accctggacg attctcgacg
      781 tgctgcatcg ggagagcctg gagaagctgc tcaccaagtt cggcatcacg gagctcgacg
      841 aggccgagaa ggaccacctc aacaaggtct ggcatcgcct cgcgccgtgg ccggacagcg
      901 tgccaggact gcagcggctc aagtcgcgct acgtgatcgg aacgctgtcg aacggtaacg
      961 tcggcctgct gacgtggatg gctaagaatg ccggcttgcc gtgggacgtg attctcggtg
     1021 cagaggtggc tcgcgcctat aagccgctgc cgcaagccta tctccgcacg gcggaaatcc
     1081 tcgggctgag acctgagcag atgatgctgg tcgcagccca taacgacgac ctcgccgctg
     1141 cggcagccct gggctacgcc acggcattcg ttgcgcggcc gacggagtac gggccgcatc
     1201 aggtgaagga cttcacggcg gaccgcgcgt gggatgtcgt cgccgacagc ttccttggcc
     1261 tcgccgatgc cctggggtgc ccctgagcat cgttcactcc ggaaagagct gcgtctgccg
     1321 caaggcttcg cccagcacca tggcagccga tacggcgaca ttgagcgagc gcagaccggt
     1381 tcgcatggga atgcgcacac tcgcatcggc caaggcgcgc acactctccg gcacgcccgc
     1441 gctctcgcgt cctaacagga gggtgtcgtc ggaggcgaac ctgaactttg tgtagggatg
     1501 ctcgccgcgg gcgctgagca ggatgaggcg gcctggcgaa gtcgccttgc gacgcgatgc
     1561 aacgtactcc tcccagctta cgtggcgcgt gagatcgacg tagtgcaggt aatcgagggc
     1621 agcccgccgc agcatgcgat ccgaaaggtc gaaccctgcc ggaccgatga cgtcgactgc
     1681 gacattcatg cacgccgata gccgaaggat cgtgccggtg ttctgcggaa tgtcaggctg
     1741 atagagggcc agccgcatga gcctgctccc ggaaaccccg tctgtagtcc tccggccgcg
     1801 cttgaacggt atgcgccaaa ttgcctcttt gccgcagggc cccggcgcct tgagaccatg
     1861 aacaaacggt gccaaaaggt cagcgtggca aaaagcgccg ttctgagcgg gggatttgcc
     1921 gacttatagg ttgcgacggt cgaaaccgtc acgtcacgag cgcggatccg gttttctggc
     1981 cgccgcgggc gagtggtggt gcgggccaaa tccaagacgg tgctcggatc gctgcgagtt
     2041 cgagacattt cggagaaggg aggctcaatt gagctcacac gccgaggagc caacgcgtcg
     2101 ggattttctg acgatcgcgg ggagtgcctt cgcggccgtc ggcggcgcca tggcgctgtg
     2161 gccgttcatc gctcagatga accctgatgc atccactcag gcgctcgcct ctgtcgaggt
     2221 cgacctgacg ccggttaagg aagggcaggc gattacggtc atgtggcgcg gcaagccggt
     2281 gttcatccgc aaccgcaccc ccgaagagat ccagaaggcc gttgccacgc ccgtgaacga
     2341 ccttcccgac cccgagcccg acaatgcccg cgtgaagaag ggccacgaga actggctcgt
     2401 gctcgttggc atctgcaccc acctgggctg catccccaag ggccagagcc tcaatgacgt
     2461 gaagggagat tacgacggct ggttctgccc ctgccacggc tcccactacg acacttcggg
     2521 gcgcatccgc aaaggcccgg cgccgcgtaa tctcgaggtg cccacctacg agttcgtttc
     2581 cgatacgaag atcaggatcg gctgaggatt acgaacgcca tgactacgca tcagtcgacc
     2641 tacgagccga agtccagcgc agagaaatgg ctggatgaac gtctgcccat catgcgcatg
     2701 atgcatgcgc agttcgtgga ttatccgacg ccgcgcaacc tgaactatct ctggacgttc
     2761 gggggcatcc tcaccgtcgt gctgggcctg cagatcctga ctggcgtcgt gctggccatg
     2821 cactacatcc cgagcgcgac cgaggccttc gattccgttg agcgcatccg ccgcgatgtg
     2881 aactacggct ggctgatccg caacctgcac gccgtcggcg catcgatgtt cttcctggcc
     2941 gtctatgttc accttttccg cggcctttat tatggctcgt acaaagcgcc gcgtgaggtg
     3001 ctgtggatca tcggcgtcat catctacctc gtgatgatgg ccactgcctt cctcggctac
     3061 acgctccctt ggggccagat gagcttctgg ggcgccaccg tcatcaccaa cttcttctcc
     3121 gccatcccac tggttggtga gagcatcgtg caatggctgt ggggcggcta cgctgtgtcg
     3181 ggcgttacgc tcaaccgctt ctacagcctg cactatctgc tgcccttcgt gcttgccggc
     3241 ctcgtcgtcc tgcacatctg ggccctccac gtggctggcc agaacaatcc gaccggcatc
     3301 gagccgcaga ccaagtccga aaccgtgccg atgagcccgt acgcggtcgc caaggacttc
     3361 gtcggcatgg cggtgttcgc gatcctgttc gcttggttcg tgttcttcgt gccggactac
     3421 ctcggccacg cggacaacta caatcaagcg aatccgctgg tgacgccgcc gcacatcgtg
     3481 ccggagtggt acttcctgcc gttctacgcg atcctgcgcg cgatcccgtc gaagctcggc
     3541 ggcgtggtgg ccatgttcgg cgccatcgcc atcctggcgt ttattccctg gctcgacacc
     3601 tcgcgcgtgc gttcgaccaa gtaccggccg ctttatcgct ggttcttctg gctgttcgta
     3661 ttcacctgcc tcgcgctggg ctatctcggc tccaagccgg ctgagggcac gtatgtgacc
     3721 tgggcccggg tgttcacttt ctactacttc gcgcacttcc tgatcgtcat gccgatcatc
     3781 ggtgtgatcg aaacgccatc aaagatgccc cgctcgatca gcgaagccgt tcttggcaag
     3841 aagggcaatg gggtaagtgc ggtttcgccc gcttcggcga ctgctgcggc cgagaagcgg
     3901 taaggctcgg ggaggcgagg tgaaaagaat gaccaccatg aggaaatctc tgcaggtgct
     3961 gatggcggcg ggtctgctgg ccatcacggc ccaggccacc ccggccgccg aagccgtcca
     4021 gatcgagcgg cagagctggc cgttcagcgg cttcttcggc accttcgaca aggcgcagct
     4081 gcagcgcggg ttccaggtct ataccgaggt ctgcgcgagc tgccacgagc tgagacgcat
     4141 atcctaccgc aacctcgccc agccgggcgg tccggagttc ccggaggatg ccgtcaaggc
     4201 cctggctgcg gcagccgagg tcgaggacgg gccggatgac acgggcgcca tgttcaagcg
     4261 cccggggctc ctttcggatc cgctgcccaa gctctacgcc aacgagaagg aggcgcgctc
     4321 gatccacaac ggcgcctacc cgccagacct ctcgctgatg gcccgcgcgc ggaatacgga
     4381 gaacgttgct ccttggtaca tccatccctt cctgatgctg cgtgacatcg ccgtcggcta
     4441 tcaggaaggc ggggccgatt acatttattc tctgctgacc ggctacgagg atcctccggc
     4501 cggcgtgcag gtcatggagg gcatgaacta caacgtcgct ttcccggggc accagatcgc
     4561 gatggcaaat ccgtttgccg ggggcgacgg tcaggtggaa taccaggacg gcaccccctc
     4621 gacggtcgac aactatgcgc gcgacgtggc ggcattcctt tcctggacgg ctgacccgcg
     4681 tctcgaggag cgcaaacgga tcggctgggt ggtgatcctc tacctcatcg tcaccgcggg
     4741 cctgctctac gtcggcaaga agcggctctg gtcaaggctg gagcactagg ctaaaggcta
     4801 ggcactcgcc tggaattgac ttgaaagggc ctcctcagcg gggccttttc tgttcagggc
     4861 tggccaggga caagcatttc gggccaggcg cagcatcacg aggcatggga tcatgacgcg
     4921 ggcaattctc ggcatcatcg gcggcagcgg gctctatcat ctgccgaaca tcaagaaccc
     4981 gcattgggag aaggtttcca gcccttgggg cgagccatcc gacgaaattc tgttcgccga
     5041 ggtcgacggc ctgccgatcc gtttcctacc ccgccacggc cgcggtcatc cgcgtgcgcc
     5101 atccggcatc aactaccgcg ccaacatcga tgccttgaag cgaaccggcg tcaccgatct
     5161 ggtctcggta tcggcatgcg gttccctgcg cgaggatctg ccgccgggca aattcgtgct
     5221 cgtcgaccag ttcatcgacc gtacgttcgc acgcgagaag agcttcttcg gcaatggcct
     5281 tgtcgcacac gtctccatgg ccgacccagt cagccctggc ctggtggatg aaatcgagtc
     5341 ggcgctgaaa gcagagcaga tcgattaccg tcgcggcggc acctacctcg tcatggaggg
     5401 cccgcagttc tcgacccgcg ccgaatcgaa cctctatcgt tcctggggct gcgacgtgat
     5461 cggcatgacc aacatgccgg aggccaagct cgcccgcgag gccgagatgt gctacgccac
     5521 cgtcgccatg gtcacggatt acgactgctg gcacgacgga cacgaggccg tcgacgtcgc
     5581 cgccgtgctc gaggtcatga aggcgaacac ggagacggcc cagcgcatgc ttgcccgcct
     5641 caccgcctca ttcccgcgcg agcacaagcc atgcccgatc ggctccgacc gggcgctcga
     5701 ggtggcggtc atcaccgcgc cggaagcgcg cgatccggaa ctcgtcgcca agctcgacgc
     5761 ggtagcgggc cgaatcctca aaccgcaggc ctagcgccgt ggcctactcg gacttgccct
     5821 cagccacttt ttccatctcg gcgagtgcga cgagcgagtg cgcataggcc ccgcccgcac
     5881 gcatctcgga cgcgacccag atcgcttcca tgatttcctg ctccgtcgcg ccgtgacgga
     5941 gcgccgcctt ggtgtggccc ttgatgcagt acgggcactg cgtgacgtgg gcgacggcta
     6001 cggcgatgat ttgcttcatc ttggctggaa gggcgccgtc tgagaagacc tgctggctga
     6061 aggcacggaa ggccgcctcc gtatcagggg cgagttgccg gcgcttttga accaactgcg
     6121 gcgtcgatgg gggatatacg gcatcggaca tcttacacct cccggctggg ttcgaagtcg
     6181 tccggcacgt taacatgtat cgggcggaga tcatatgggc acgaagcaag gcagccgctc
     6241 atagaacgcg cgataagggc gccagaatca gagctggcaa aattgcgcgc ccgggttgcc
     6301 tagccgggtg gaaacagaat aaggatctgc atcttcacga gatcggggaa ccaattcagc
     6361 aacacgtgcc ctacgcccca gagcagcaaa ccgagcacga taagcgccgg caccgcggca
     6421 aataccgcct tcaggaagaa ggccgtcagg tgaaggaatg gaagatcgag ccgggtaatg
     6481 gtgcccggag gtgcgcttgt tccttccgac acatgcgggc cgaatccgtg gtaggccatt
     6541 gcggcagcgc gctcccgcat ttcgcgggcc tgccgctctc gtgcttggcg ctcgcgggct
     6601 tcgcgctccc ggcgcaacgt gcggggcagg tcatccgggc cgccatccgc gacggggatc
     6661 gaaaaaggag ggtcagaaat ggccatatgc aattacctcg taccgccggc ttgcgagcgg
     6721 acaatcggcc accatccccg gcaacgtggc accgcgccct cgcgctactc cctaccgtca
     6781 gagaaccgat aatgacgatt tgcgccgcca atagggcatc gaggaggtaa cggtggacga
     6841 gctgaagcag ctgatccgga ccatcccgga ttatcccaag cccgggatca tgttccgtga
     6901 cgtcaccacg ctgatgcgcg atgccgatgg tttcgagcgg gcgatcatcg cgctcgccaa
     6961 gccgttcgaa gcgcagaagg tcgacgcggt ggcaggtatc gaggcccgcg gcttcattct
     7021 cggcggcgcc gtagcggacc ggctcgggtg cggattcatc cccattcgga agaagggcaa
     7081 gctgccgtgg aagaccatcg gacaggaata cacgctcgag tacggcgtag acgcgatcga
     7141 aattcacgag gatgcgatcc gccaaggtga gcgcattctc atcatcgacg acctgattgc
     7201 caccggcggc actgccgatg ctgccgcgaa gctcgtccgc cgctcaggcg gcgaggtggt
     7261 cggcgccgct ttcgtcatcg acctgccgga gctcggcggt ctcagcaaat tggaagccag
     7321 cggcgtgaag tgtcacgcgc tggtcgcctt cgaaggacac tgaatgagcg agatgaatgg
     7381 aaacagcctg ccgccgggca ggattcgcga ggcattggcc gccgtgaacg ccgtccgcct
     7441 gaagccggac tccgccgtgc cctgcccggt ttgcggcaat cccgggtttt cggtcgagga
     7501 tcgctccgcc cggccgcatg ccgaatggta tgcgctgtcg tgcacctctt gcggtctcca
     7561 cgacaacctc cacattccga tgggatcgac gccgggcagc ttcagctgat cgcgttaatg
     7621 tgagctgggc cgtcatcctg ggccgtgtgc ctgggatcat accagccacg aacgccggag
     7681 taagcgtcga gctggatccc gggaccagca ccgggatgac gacctgagtc gagagccgat
     7741 gcgttccaac cactggcgga gtgcgttttg gctcggctgg attcgaaaac ccggataacg
     7801 agcggacggg tcgggatgaa gatcggcggg cggcactacc ggaccatatg gctgaaccca
     7861 gatggatggt cggtcgagat catcgatcag acgaagctgc cgcatcggtt cgagacggtc
     7921 acgctcacgt cttgtgagga tgccgcgcgc gccatcctga ccatgcaggt gcgcggcgcg
     7981 ccgctgatcg gtgtcactgc tgcctatggc ctctgcctcg cgctccgcga ggacgcctcc
     8041 gacgaggcga tggagcgcgc gatcgaacac cttgcccggc aacgaccgac ggcgatcaac
     8101 ttgcgctggg cactggagca ggtgcgcgcc gccgtccgta acctgccgcg cgaaaagcgg
     8161 gtcgaggctg cttacaaatg cgccgccgcc ctggccgacg aggacgtcga gacctgtcgt
     8221 cgtatcggcg tcaacggcct cgcgctcatc aaggatatcg ccgcgaggaa aaagcccggc
     8281 gagagcgtca acgtgctcac gcactgcaat gccggctggc ttgcgtgcgt ggactggggc
     8341 acagccacct caccgatcta tcaggcgcac gatgcgggca tccccgtcca cgtgtgggtc
     8401 gacgaaacgc ggccgcgcaa tcagggcgcg tcgctcacgg cctatgagct cggcagccac
     8461 ggcgtgccgc acgccatcat cgtcgacaac gcgggcgggc acctgatgca gtccggcaag
     8521 gtcgacattt gcatcgtggg gaccgatcgt gtgaccgcga acggcgatgt cgccaataag
     8581 atcggcacgt atctgaaggc gctcgcagcc aaggacaatg gcgtgccctt ttacgtggcc
     8641 ctgccgcatt cgacgatcga ctggacgctc gatgatggcc tttccattcc catcgaggag
     8701 cgctctgagg acgaggtcct caagatgccg ggccgcctgc cagacggatc ggtcgtgacc
     8761 gtcgagatcg ctgcaccagg ctcacctgcg ggaaatccgg ccttcgatgt gacgcccgcg
     8821 cggcttgtca ccggcttcat cacggagcgt ggcgtcgccc cggcttcacg cgaagggctg
     8881 ctctcgctct accctgagcg caaagacgca tagcgcgttt ccattctcgc gcccagttga
     8941 cttgaccgtg gcgtccgatg gcctttgatg accgcagccg tatggagaca tgcccatgct
     9001 cagtcgagtt ggtcttgccg ccgctctcct cctctccctc ctcgtgcccg ccgcggcgca
     9061 tcacggatgg ggtagctatg atgcgagcaa gctggtcacc gtcgaaggca gaatcctcac
     9121 ctcgcaatac gccaatccgc acgtgacgct gacagtccag ggcaaggaca agacgtggac
     9181 tctgaccctc gcccccgtct cacgcatgcg cagccgcggt gcggagcagg agttgttggc
     9241 gcccggaaag acgatcacgg cagtcggtta cgtctcgacc cgcgatcctg acgagatgcg
     9301 ggccgaacgg atcacggtcg acggcaagac gatcgagatg cgctagatgc tgcccgaagc
     9361 gccagccatt ttcatagcgc tggagcaatc aggcgtggga gcggcgatcc ggcaatcggt
     9421 gtggatctat cccatcgcca acgtcacaca catcctggcg ctgaccgtgc tggcggcgtc
     9481 ggtcgccgtg atggatctgc gcctgcttgg ggcattccgc gagacggtac ctgccgccgt
     9541 gatcgtcccc gcgcgacgta tctcaatgct ggccctgacc atcatggtgt tgactgggct
     9601 cgtgctgttc acggccgagg ccagccacgt ggtcatgaat ccggtgtttc agaccaaaat
     9661 gggcctgatt gcactcgcca tcctcaatgc cctgatcgtg cagcgcacgt tgcccgccgt
     9721 gctgataaaa acgccggcaa tgacacctct gccgccgcgc ttccgcgttg cggccgtcac
     9781 atcgctcgca ctctggttct gcatcgccgc cgcgggacgg ctgattgcgt acttctagat
     9841 tgcgctgtgc attgatggga gaggcagctt ttccccttac caaattgggc gaggactgac
     9901 ggggcaacct aagccgccgg gttcagcggc gcgaagtgct cgcggacggc ggcttcctcc
     9961 agatcgcgcg taatgaagac gatccgtgaa cggcggtcgt ccgtaggcca gcgttccagc
    10021 atttcgacgg gatagaacac atgctgcacg ccatggatga cgaacggctg gtccgcctcc
    10081 cgtacgttca gtagtccctt gatccgcagc agcttgctcc cctgcgtcgc acgcagaagc
    10141 tcgatgaggg cttcgagctt gctggcctcc agcggttcgt tgaatgtcag gcagaacgag
    10201 cggatcccgt gcagatggcc gtgatcgtgg tcgtggtcat ggtcatggtt atcgtgcgga
    10261 tgatcgcagc cctcatcgtg agttgcatag cgatctgccg ccagccagcg actgacatca
    10321 gtgccgtcgc gaatttcata agcgttgaga tcgaagattt gagacggctc gatagcgcct
    10381 ttcactgcct gcaggacggg cgcggcagga ttgagaaccc gcacctgcgc cgtcaattgg
    10441 gtgatcccat cagcatcgac gaggtcggtc ttggtgagca cgacgcgatc cgccatcgcg
    10501 atctggcggg cgctttcgaa ggtcctgtcg agctgatcga ggccgtgctg cgcatcgacc
    10561 gtcgtgatga tgccgtcgag gcggtagatg cgaaagaggt cccgatcgtt gaccagcgtc
    10621 tgcgcgatcg gcgccggatc agcgagccct gtcgtttcca gcacgacgcg cgtcaggtcg
    10681 gggatggcgc cgcgcatcat atcatcgtgc agcttcacga gggcattgat gagatcgccg
    10741 cgtacagcgc agcacacgca ccccgacggc agcaggaccg tatcctcctg gacggcctcg
    10801 atcagcgcat cgtcgaggcc gacctcgccg aactcattga cgatcaccgc ggtgccgcca
    10861 agctccggcc gtttcagcat tgccttgagg agggtcgtct tgccggagcc gagatagccg
    10921 gtcagcacgg tgagcggaat ggggaggttc aaggcggcgt actcgcgatg tctgagcttg
    10981 ggtgccaggg ctcgcacacg cctgtgtgga gttcaagtct cgtttgggtg cgcttgcacc
    11041 ttcgatccca ccagcgcttc aaactcgcgg acgaaccgct tcaggctgcc ccagtccgtc
    11101 agctccgtat caccagaagg gtggcgctcg acgcctttcg agtcgacgat ggcatggagg
    11161 atcaagcgct cgaacgggtt gagcctcctg tcgtggaccg caccggccac gtgacagacg
    11221 atgtcgggca tccaaccgtg ctcggcaacg aaccgttgcg caatctcgtc gattgctgat
    11281 cgctcgctct cttcatgcga ggcagcagac aggctgaccg gcagaaaggc cgaagggcaa
    11341 cgccgcaaag cctccccgtg ccgcatgatg aaggaggaca gctcgccgtc gaactggcca
    11401 cggtgcaccg acccggcgat caccatcgcg tcgcagtcgt caacgccggc ttccgtctcg
    11461 tgcgccttga tgtcgacgag ccgcaccagc gcgccatcat cctcgaggcg gcgggcgacg
    11521 aagtgggcga ccttttcggt ctgcccctcg cgtgagacgt acgcgacaag ataacgcagc
    11581 atggggcgag gtccttacct gatggcgaca gcccacccca gagacattgg agccgctttg
    11641 actgcaatca accttcgaat ggcctgcgcc gctcgacgaa aaccttgctg acgaccgaga
    11701 agacgagctc gttgttctgg ttgacggctt cgttgtccga cagaaccagc ccgacatccg
    11761 gccgggagcg cgagtcgatc ttctccttcg cacgcgtggt gaaggtgagc gtgtcgccga
    11821 catacaccgg tttcagccag cgcagcttct cgaagcccgg ggacgggccg tagcgcggcg
    11881 gccgctgacc ctgaaacctc atgaggtccg cctcgcggtt ccggtgatca atgaggtgcc
    11941 gcagccagat ggcgctcgtg tgccagccgc tcgcgcacag cccaccgaag agcgtgtggc
    12001 gcgccgcctc ctcgtccaag tggaagggct gcgggtcata tttgcgggcg aaatcgatga
    12061 ttgcctcccg cgtgaaagta tatgagccga gatggacggt agcgccgatc tcgatatcct
    12121 cgaaatagcc gctcattggg ccacctccgg cgcacgcaga cggaagagtt gcgccacatc
    12181 ccaggtcatg accacctcgc cgttctggtt gagcatctcg aacagcatct ggcagatccc
    12241 cacgccgggg cgcgatttca gcacccgctt ggacgtgcag gtatagcgcg cagtcagctc
    12301 gtctcctgga aagacgggct tcagccattt cacctcgtcc agaccgggcg agccgagccc
    12361 cgcggcattc gccagcacgt catccaccag catgcgcatc agcatggcac aggagtgcca
    12421 tcctgacgca cacaggcggc caatcatcga ggcctccgcc gccgcctcgt cgatgtgaaa
    12481 gggttgcgga tcgaaggccc gcgcgaagcc gatgatctcg tccttcgtga cccgcttgcg
    12541 gccgaaggcg acggtctggc cgacgattac gtcctcccaa tgaagggagc ctgcggtggt
    12601 tggatcgcct ctgctcatgc gcctcaaccc actccgatgc tgtcaagact tgcccgtctg
    12661 atagcggcga gcggccacgg gggaaagatg ccgcgcgcga ttttgccggg attcgccgcg
    12721 ccggaacgcc gcaaaccgac caccagatag gctcattcgc gccataaact cgccaatctc
    12781 cagtgttcga tgagcaacgt gatgcagaag taagaaaagg ggcttcacgt gttcagtaac
    12841 actaaagcat tacgtcagtg gtcaaccatt cgacggtggg gatgttcggg ggcagcttcc
    12901 gtgatcatct tatccatggc cgcaattact ccgacgtacg cctttccgaa gctcagtaat
    12961 ccaacatcta ttccaactga gcagccgaac gaccaggccg agccggagac gaaaaacgag
    13021 gccaaggaaa cccagtcggc aattgacgaa tacttgcaca agctgatgat ggcggagtcg
    13081 gccggtaagg ctcacgcgaa gaacccgcgc tcgtcggctc tgggtcctta tcagttcatc
    13141 aactcgacgt tcatcgaagt ggtcgatcgg catttctccg acgaagtcga ggggatgagc
    13201 cagtccgaga tcctggcgct cagaaccgac atggagttcg cccgccgggc tgccatcgcc
    13261 ttcaacaacg acaatgcgaa gcaccttcgc gagaacggcg tgaaaccgac cttcgctcac
    13321 ctccggttga gccatctgct tgggcccaac gccgccgtga aggtgctgcg cgcaagcccg
    13381 agcaccaagc tgacgaagct gctgccttca gcggtgatca aggccaaccc gttcatgcgg
    13441 ggcatgacgg tcgccaatct ggtccgctgg tctgagggtg aggtcggcgt cggcgtgtgg
    13501 gctaccccga ccgagatcgc caagtcgggt gagagcaagg ccccggcggc cacagaggtc
    13561 aagaccgaaa cggtgccgcc gctgcctgaa aagaaggttg aagcggaccc tgtcgagggc
    13621 accaaccgga cactctaggc cgttacgcca ctctcccccc aaaaggtgtc acggttccgc
    13681 cctcctcccg atgcgaggag ggcggtttgc gttcagggcc ttgcgtgcgg gaccttgcga
    13741 aggcgaagag ccgcccgacc ctggctcaag tcgcgaagcg gaagtgcaac acgtcgccat
    13801 ccttgacgac gtaatcctta ccttcgagcc gcatcttgcc ggcctctcgc gcgccgacct
    13861 cgccgccgta ggccacgtag tcgtcgtagg cgatcgtttc ggcgcgaatg aaacccttct
    13921 cgaagtcggt gtgaatgaca cctgcggcct gcggcgcctt cgtcccctcg ggaacggtcc
    13981 aggcccgcgc ctccttgggc ccgacggtga aataggtgac cagccccagc agccggtaac
    14041 cctcccggat cagccggttg agcccgggct ccttcagccc aagctcggcg agatagacct
    14101 cccgatcctc cggcgaaagg ctcgcgagct ccgcctcgat cttggcggag atgatgacgc
    14161 aacctgcgca ttcctgcctg gcgcgctcct ccacctggcg cgagtaggca ttgccggtcg
    14221 ccgccgatgc ctcgtcgacg ttgcagacgt aaagcacggg tttagcggtc aacagctgga
    14281 gctgccggta gagcggctcc tcctcaggcg tcagcgcggc cagccgggcg ggcttgcctt
    14341 cgcgcagcag cgcgagagcc ttgtcgatga tcgcaagctg cgccttggcc tccttttccc
    14401 cggacttggc tttcttttcg agcgggatgc gccgccgctc caggctttcg aggtcggcca
    14461 gcatcagctc ggtctcgacc acctctgcat cggccaacgg atcgatgcgg ccctcgacgt
    14521 gcgtgacgtc gggatcctcg aagcagcgca gaacataggc gaccgcgtcg acctcgcgga
    14581 tgtgcgccag gaactggttg ccgagcccct ctcccttcga agcgccacgg acaagacccg
    14641 cgatgtcgac gaacgtcagc cgcgtcggca ctttctgggc gctcttgccg atccgcgcga
    14701 gcgtatcgag ccgcgggtca ggcacgccga cctcgcccac gttcggctcg atcgtgcaga
    14761 acggatagtt ggccgcctcg gcagccgccg tctgcgtcag cgcgttgaag agtgtagact
    14821 tgccgacgtt cggcaggccc acgatgccgc atttgaagcc catgaagtcc aatcctgtcc
    14881 gggaaggccg gccgggagaa ccccggtctc gacacccgag gcatctaagc gcttgcggcc
    14941 ggattgaaaa ccccttgcgg tgaggcacga agcccgcggc gcgccagggc tcctggtgtc
    15001 agggtgtgcg gcaagccgct gctctattcc caggccccgc cgaaactggt atgggagcaa
    15061 ggatctcatt gctcgctctg acctctcatg gacctcagct cgctcttgat attcgccggg
    15121 gcactgctgg ttgccgccgc gtcccctggc ccgggtgtcg ccgccatcgt cgcccgcgtg
    15181 ctcggacgcg gcatgcaggg cgcctttgca ttcactgccg gcatggccct tggcgatctg
    15241 atctggctgt catgcgctgt gctgggactg gcggcgattg cgcagacctt cggaggcgta
    15301 ttcctcgtca tcaaatatct tggtgccgcc tacctactct atctggcctg gaagctgtgg
    15361 acaggcccgg ccgacgcgct ggaagtgaag gtcgatcggt ccgacgcaaa gccgctgcgc
    15421 ctgttcctcg ggggcctggc ggtgacgatg ggcaatccga aggtgatgat cttctacctg
    15481 gctctgctgc cgaccatcat cgagatggca cgcgtaagct ggctgggctg gctcgagctg
    15541 tcgatcgtga cactggccgt gctggccatc gtgttcagca gctacgtact gctcgcagtc
    15601 cgcacacgaa aactcatcgc caacacacga gccatgcggc tcctcaaccg cttcacggga
    15661 gccgtcatgg cctccgccgc cgcggccgtc gccagccgct gaaggctact tcaaagcagg
    15721 ctgaccttaa gaggcatgcc gcccttgggc cgcagcgtga cgcggctcac cggctctggc
    15781 agatgcttgc cgtcccactc gaaacgggct cctcgcacca gcgcggtcca gcctgcctgc
    15841 ttggtgggga aagcttgcgc ctcagctggc cggagccgat gcgctgcggg cctttcccgc
    15901 ctccttcttg agacgcttgc ggactcccgc cgtattgccc tgaatgcgcg tgaccttcgg
    15961 ccgggcgatc ttgcggctgg cctgcttctt acgggccaat gtgtttctca gcgatcttgc
    16021 ggacatgggc tcctccacga atgcatcccg cagcctgctc ttcacgcatt ctgcgccagt
    16081 tctccgggca acaagccccg acaccttagt tgacaatgaa gctaatgcca tatccgggcg
    16141 ccaggaaggc gcccgcccag atcaggcagg atagcccact cgagggtagg aacagggccc
    16201 acggcatgct cagcataccc gcgacgacgg gaacgaatgg ccgcaacccg ccaatgaact
    16261 tgcccagcac gatgccgaga acgccccatc gctcgcagaa catttgcccc ttggggatga
    16321 agccggggaa cttgcgcatg ggccagacct taccggcatc gtccttgaag tagcgcccca
    16381 gagcgtacga gacgcagtct ccagcccatg cgcccgtcgc cccggcgacc caaatgggcc
    16441 agaactcgcc gccggcagcg ctgtggatgg cgccgatgcc caagaacaaa accgatgatg
    16501 gaacgaacag cgaaatcagt acgatgctct cggtgaagcc caaaacgaag accagcagcg
    16561 cgatgaaccg cgcatgctcg ctggcaaaag tgagcaggta ctcggtccag ccgccggccg
    16621 caaaaaagct tagcacttgg ggataccact gattgaagat gcttcgacta ttcgaacgac
    16681 tggacggagc ctgtcggctt ataaactgct actacatcga aatgacgcca acccgtatgc
    16741 attcgggtca cgcccccatc agccatctcg cgccaaaggt gctgggcgcc agaacgccgg
    16801 tggcccagag gaaagcggag ctcacgttcg cgatctgaaa cgggagctgg cgcatggcgt
    16861 acatgccggc cacgaccgga atgactgcac ggatcgggcc gaagaagtgg ccgaggaaca
    16921 cgccgaagac gccgtatctg tcgaagaatc gctgcccctt cgggatcatc tgcgggtatc
    16981 gtttgaatgg ccagaccttg tgaatggaat ccttgaaata gagaccgatc cagtaggaaa
    17041 tggcatagcc cagcgagccg ccaatcccgg cagcaagcca gaccggccag aagggaatac
    17101 cactggcgcc gagaaggccc ccgatggcaa ccaggaaaac cgtcgagggg agcacgagtg
    17161 acaggaacgc gacgctttca ccgaatgcga gcaggaagac gatcggaccg gcccagatct
    17221 ggttcgcgcg aatgaacgcg accgtcggct ccacatactg cgtaagatcc atcgtctggt
    17281 gccctgtccc acccctccaa cttgtgctca gctaggttcc tttgccgccc gacgcaagtg
    17341 ccgtttctgc gcgcaggcct gtcaccccgc cggcgcgcga tcgcaccaga agcgatacat
    17401 cgcctcgaaa acctggggcc ggtcgcgttc aaacgcctgc caatcggcca gcgatcccgg
    17461 ccactggctt tcgggatact ttctggcgaa cgccgccagg acgagcggat cgagcgtgaa
    17521 gccgcgaaag gcgagcccgt tctcgtcaag gaagcggctg atctcgtcga gcgtcacgtg
    17581 ctgctcggag gcgtggaggg ccaagtctcg gaactcgtgc aaggtataga agtcccgcga
    17641 aacaacaagc tcggtgccag gtgagcccgc gggacggtcg aggagctgcg ctcgccaggc
    17701 ccgtgccgtc gcatcatcgc agtcgggtcc gggataatcg ggctgctcgc gcaaaatgcc
    17761 gaagttcgcg cgcgcggtcg cgctgtagag cccgacgtac atcagcccgc ttggcttcag
    17821 ccgcgaaacg agcttgcgcc agccggccca tggatcggcc atatgatgca ggacgccgac
    17881 gcactcgatg atgtcgaact ggcggtcgag gcgatcgaga tcgagaatgt cggccaccat
    17941 gaactcgata ttgtccaccc cataccgttt ggccgcgcag tgcgcatagc cgaggctcgg
    18001 ggccgaaagg tctgtcgcaa gcagccgcgc ctttgggccg taggcgaaag cggactgcag
    18061 ggcctgcttt cctgtccctg cgcccgcgat caggacatcg aattccccgt ccatgaacgc
    18121 caggcgctgc ggctcgaagt agcgcgacag cgtcgccttc atcgctccgg gttgcgagac
    18181 atggaggctc tgccagcgcg gataagggct cgactcgtac tggcgcgcga cgcggcggga
    18241 ggtttcgtcc gtcagtggcg cgagccgggg aatgcgttct gcggcgtgct tttcctccgc
    18301 gcggcgctca agctctttct gtaccacctc gcgcagcggc ttgggcttca catcgccgta
    18361 gtcctccgcg ttcaacgagg acagaatggt ctcgagcggg cggtagagcg cgaccagcag
    18421 caaccgccgc gccatttcca ggtcgccctt cagcagagcg gcggtgtcga tttccatccg
    18481 ggcgagggct tcggtctcgg cctgcgattc cgcccacacg tgatcgttgt tccaggcttg
    18541 tgcgagcagc cccagggcaa agccacagag cgtgcgatcc tcgaagcgct ccgccggcac
    18601 ctcgagcagc aaaatgcgcc gcagcgccgt caacagccgc tcgagccccg cttccatgac
    18661 gacaccggcc tgcagcgccg atagcagcag atccgacctc agcacgtccg cagtgcgatt
    18721 gaggatcaac ttccgcgctg tctcatccgc atgaccgtcg cttacagctt ccagtgccct
    18781 ggccagctcc ggcgtcaccg cgacgagata cttcagcgcg gcctcggcaa tcggctgcgt
    18841 gtcgaccccg ctgattccga gcgctgcctt gagcccgaac ggctcgaggc ggctcgcatc
    18901 cgtgatcgcg aaccgggcca gaaggcgcga caggcgcttc gccgcttcct catgctgcgg
    18961 tttgaagcgc agggcttggg tcagatgaac gaccgactcc tcgatctcac ccagggccgc
    19021 cagcacggcc gcctgctcga agtgcaactg cggattgcgt cggtcatgac gcagcgcctc
    19081 gatgtaagca gctcttgcgc ccttgaggtc accggagcga aaggcgcgcg tcgccagcgc
    19141 cacccagtcc tgttttgcgg ccggcctgcg gatcggagct gtcatcgtgg gcacgtcatg
    19201 ctggctgggg tttcaaggtc taagctctgc agcgccgctt catttcggcc gcaagctcac
    19261 gcgagacctc ggcgccgagc atgaggccac gggaaacctg cgccggcgtc agatgcgagg
    19321 ctccaggccc gcgaagcact gccggggcgt tctccgcttc gcggcggccg atggccagct
    19381 catcgcacga gagctccgcc ttgcgcgttt tgaatgcgaa gagtcgcacg cccgaggcat
    19441 acgcttccga tgttggcggc tcgcgtaccc aggcgcggct cctgtccgac atgatggcat
    19501 tgagcgcgat gcggcgctgc tcgatgcagt gactggaatc gtccacgcac cccatgccca
    19561 cctgtgacgc ggaattgaag ccggcgctgc acgccgcagc cgccattccc atcagggcgc
    19621 aagtcagcct ggatgtcgcc cgccgcgcgg ctcgcctgcg gatcatggtt cgccccttgt
    19681 gaagatcccg ccaagcccat agaaggcagg gatggcggcg gtgtggcgtg gtctttgcgc
    19741 attcgataca gcatcgccgg ccccgcgcaa gatttccgac gcgcagttca cggcccttta
    19801 tctcaagagg aaactgctca tggtgcacgc aatccggatt caccagcatg gcggccccga
    19861 ggtcatgact tgggaggaga tcgaggtcgg cgagcccggc gagggccagg tacgcctgaa
    19921 gcatcgcgct atcggactca attacatcga cacctatcac cgctcgggcc tctacaagat
    19981 cagccttccg gcggtgatcg gaacggaagg tgccggtgag gtgacggccg tcggccccgg
    20041 cgtgaccgac ctcaaggtcg gcgaccgggt ggcttatgcg agcccgatgg gcgcctacac
    20101 ggaggagcgg ctcatccctg ccgatcgcgt cgtgaaaatc ccggacggca tcgacgatca
    20161 gacggcggcg gccatgatgc tacagggcat gaccgtccgc tacctgctca aggaaaccta
    20221 taaagtcggc ccgcagacga ccatgctgct gcatgctgct gccggcggta tcggtctcat
    20281 cgcctgtcag tgggcaaaac acctcggcgc caccatcatc gggacagccg gatcgcccga
    20341 gaaatgcaag ctcgcccagg accatggcgc tacctacacg atcaactacc ggaccgagga
    20401 tttcgtcgcg cgaaccaagg agctcacgaa cggcaagggc gttgacgtcg tctatgactc
    20461 tgtcggcaag gacaccttcc cgggctcatt ggactgcctg aagccgcgcg gtttgtgggt
    20521 cagcttcggt aacgcgtcgg gcgccgttcc gccgttcgac atcctcatgc tttcggcgaa
    20581 gggctcgctc tacgcgaccc ggccgacgct cgccacctac acggcaacgc gtgaggatct
    20641 ggtggcgacc gccaacgatc tgttcgatgt ggtgagccgc ggcattgtga agatcaacgt
    20701 caaccagacg tatgcgctga aggacgccgc acaggcgcat cgcgatctgg agtcgcgcaa
    20761 gacgaccggc tcgaccatcc tgctgccctg agcccgaaac tctcagtcga ggagaagcaa
    20821 gtcatgctga agatctacgg acggacgaac tccatcaacg tccgcaaggt gctatggacg
    20881 gccgaggaga tcgggcttac ctacacccgg gaggattggg gccgcgggtt ccgttccacc
    20941 tcggagccgg a
//
LOCUS       DASVDU010000293        10408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_962298, whole genome shotgun sequence.
ACCESSION   DASVDU010000293 DASVDU010000000
VERSION     DASVDU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962298"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3819)
                     /gene="gltB"
                     /locus_tag="VF226_19665"
     CDS             complement(<1..3819)
                     /gene="gltB"
                     /locus_tag="VF226_19665"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX7076262.1"
                     /translation="MTESRFNLDALNDRFDDDEAPKGLFDPAREHDACGVGFIVDLKS
                     RASHGIIEDALSILENLEHRGAVGADPIAGDGAGILIQIPHAFLAEECAKLGFKLPPP
                     GHYAVGHIFMPRDERLRRHCETVWARIVREEGLQLIGWRDVPIDNSCLSDGVKATEPV
                     HRQVFIARPKAIRDDDAFERRLYIVRKVVSNAIYDAYKARDIGHYTVSLSSRTLVYKG
                     MFLSYQLKAYYKDLSDRRMASALALVHQRFSTNTFPSWKLAHPYRMVAHNGEINTLRG
                     NVNWMAARQASVSSPLFGNDISKLWPISYEGQSDTACFDNALEFLVMGGYSLAHAAMM
                     LIPEAWAGNPLMDSRRRAFYEYHACLMEPWDGPAAMAFTDGRQIGATLDRNGLRPARY
                     LVTKDDRVIMSSESGVLPIPEDQIIAKWRLQPGKMLLIDLEQGRIISDDELKSELAAR
                     NPYEQWLRRTQIQVSDLPQPKMPKRRKSNIDLLDLQQAFGYTQESLKFLMAPMAQTGQ
                     EAVGSMGTDTPISALSNKPKLLHTYFKQNFAQVTNPPIDSIREELVMSLVSFIGPRPN
                     LLDLEGTSKLKRLEVGQPILTNDELERIRQIGDVPDNHFTSITIDITYPQEQGAAGMG
                     PALDVICAEAEEAVRSGDYNIIILSDRATGPDRIPVPSLLATSAVHHHLIRQGLRTSV
                     GLVVETGEAHEVHQYAVLAGYGAEAINPYLAFDTIEQLASELGEDLSPKEAVKRYIKA
                     IDKGLLKVMSKMGISTYQSYCGAQIFDAVGLKSSFVKKFFTGTHTTIEGVGLRQIARE
                     TVERHRQAFGDVPTLQNALDVGGEYAYRVRGEAHVWRPTVVADLQHAVRGNLPEKYRS
                     YAKQINEQSEQLMTIRGLFRIKSAAELGRKPVPLEEVEPAASIVKRFSTGAMSFGSIS
                     REAHTTLAIAMNRIGGKSNTGEGGEEADRYKPLPNGDSMRSAIKQVASGRFGVTTEYL
                     VNSDVMQIKMAQGAKPGEGGQLPGHKVDATIAKVRHSTPGVGLISPPPHHDIYSIEDL
                     AQLIFDLKNVNPNAAVSVKLVSEVGVGTVAAGVAKARADHVTISGFEGGTGASPLTSI
                     KHAGSPWEIGLAETHQTLVANRLRGRIAVQVDGGIRTGRDVLVGALLGADEFGFATAP
                     LISLGCIMMRKCHLNTCPTGIATQDPVLRAKFRGKPEHVINFFFFVAEEVREYMAALG
                     FRTFNEMIGQSDVLDKDRAIRHWKARGLDFSKLFHKPEVDSSVAIYNSERQDHGLDKV
                     LDRKLIELAKP"
     gene            complement(4417..4872)
                     /locus_tag="VF226_19670"
     CDS             complement(4417..4872)
                     /locus_tag="VF226_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX7076263.1"
                     /translation="MRLDFAPLYRSTIGFDRLVQLLDSVSGFSDEAVSYPPYNIERTG
                     ENEYRITMAVAGFRSDEINVEVKDAALTIRGEKKPEEKQREYLHRGIAQRSFERRFQL
                     ADYVEVTGASLTDGLLHIELVRNLPEQMRPRVIPIGTPERAIEADVSKA"
     gene            5121..5684
                     /locus_tag="VF226_19675"
     CDS             5121..5684
                     /locus_tag="VF226_19675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076264.1"
                     /translation="MSLRLLTAGLVAALPLAAIPAASAADLGPPSDYGYSESPYDDPR
                     YADIYRDPKPPYAPPFAKPCAERDYGCYDEGYDPPAHGKWRGEYLEPLPPPPEFDEYR
                     PRKAYLPPACLPRHEIRRALIEDGWSDLHDFEFGKRKAFLTARRPNGSLYRLEVDRCD
                     GEVVRAERVEHAHAHSWSSRRKGYPTF"
     gene            5914..6870
                     /locus_tag="VF226_19680"
     CDS             5914..6870
                     /locus_tag="VF226_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7076265.1"
                     /translation="MELVAIARNPVPSGAIVGAYEGYDGNPMRFARWEPTRGQARGTV
                     SVFTGRSEFIEKYFEVVADLRRRGFAVTVMDWRGQGGSYRPLHNQRKGWVRSFAEYDK
                     DLACFMEQVVQPQCPAPYYALAHSMGGHILLRVASQPGCPFERITVLAPMLRFHDSKV
                     GTSQRLARIYAALGTICGFGRAYVRGGSDDPADPVVFEGNPLTSDEARFARNRALIEA
                     APQLLLGAPTIGWLNAAYRSMDMLNDPAYAERIRVPLMIGIAGRDVIVDPRATEAFAN
                     RVKLCTTVMLPLARHEILQEADDIRGRFWAAFDAYFGVEPVA"
     gene            complement(6901..7698)
                     /gene="hisN"
                     /locus_tag="VF226_19685"
     CDS             complement(6901..7698)
                     /gene="hisN"
                     /locus_tag="VF226_19685"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX7076266.1"
                     /translation="MSARSEIADYISTAHDFADLAGAAILPHFRRRIAIENKAAAGGF
                     DPVTVADRAAERAISRELARRFPDHGLEGEEYGQRNPDARMRWIVDPIDGTRAFILGM
                     PLWGTLIALMDGSAPVIGMMDQPYTRERFWSGQRSSHFRGPDGKERRIKTRPCPSLAE
                     AMFTTTDPHLFSSPKDQRGLKAIKERVRSVRYGGDCYAYCLLAAGMIDIVVETGLKSY
                     DVAALIPIIERAGGRITTWEGGSAASGGRIVAAGDPRVHEEALSLLA"
     gene            complement(8458..9357)
                     /locus_tag="VF226_19690"
     CDS             complement(8458..9357)
                     /locus_tag="VF226_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX7076267.1"
                     /translation="MSQRERASILAELTPPFVVREPVEQTTPFVFCSPHSGNVYPSVL
                     LERSRLDAHTLRKSEDCFVDELFGDVATLGAPLIAARFPRAYLDVNREPYELDPELFE
                     EPLPHFANTQTMRVVGGLGTLARIVADGEEIYDEPLPLSAAIERIERLYKPFHARVAQ
                     LLERTRRRFGIAILVDCHSMPSSSIHQPSGSRPDFVIGDRFGASCDAKVTRLFREVIT
                     QRGYQVQLNRPYAGGFITEHYGQPFKGVHALQIEINRGLYLNESRLTRTANFGRLRHD
                     LVQMIARIFSELPPIIAPRAAAE"
     gene            9611..9997
                     /locus_tag="VF226_19695"
     CDS             9611..9997
                     /locus_tag="VF226_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7076268.1"
                     /translation="MPAKPSRLPIVRILLAEDDESMRGFLERALTKAGYDVISFANGA
                     DAYQRLKEEPFTLLLTDIVMPVMDGIELARRASELDPELKIMFITGFAAVTLNTDPGA
                     FKDTRVLSKPFHLKDLVREVDRLLAA"
     gene            complement(10061..>10408)
                     /locus_tag="VF226_19700"
     CDS             complement(10061..>10408)
                     /locus_tag="VF226_19700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076269.1"
                     /translation="PAESFKCVVTYIPQGSVTRLQQNLRCKSANYKLDAATHLELRGD
                     KVVGRWQDNIYVGLKGTVAGVVKEGGFDVVLSGQFFTAKMTVVGSGCKQSVTVTPARA
                     DYIREVSASLSKC"
BASE COUNT         1754 a         3272 c         3382 g         2000 t
ORIGIN      
        1 gggcttcgcc agctcgatga gtttccggtc gagcaccttg tcgaggccgt gatcctggcg
       61 ctcgctgtta tagattgcga cgctgctgtc gacctccggc ttgtggaaca gcttcgagaa
      121 atcgaggccg cgcgccttcc agtgccggat ggcgcgatcc ttgtcgagca cgtcggactg
      181 cccgatcatc tcgttgaagg tccggaagcc gagcgccgcc atgtactcgc gcacctcctc
      241 ggcgacgaag aagaagaagt tgatgacgtg ctcgggcttg cccctgaact tggcgcgcag
      301 cacgggatcc tgcgtggcga tgcccgtcgg gcaggtgttc aggtggcact tgcgcatcat
      361 gatacagccg agggagatca gcggcgcagt cgcgaagccg aactcatccg cgccgagcag
      421 ggcgccaacc agcacgtccc gacccgtgcg gatcccgccg tcgacctgca ccgcgatacg
      481 gccccgcagg cggttcgcaa cgagcgtttg atgcgtctct gcgagcccga tctcccacgg
      541 cgagcccgcg tgcttgatgg aggtgagcgg tgacgcgccg gtgccgccct cgaaaccgga
      601 gatggtgacg tgatcggcgc gcgccttggc gacgccagcc gcgaccgtgc cgacgcccac
      661 ctcggagacg agcttcacgg agacagcggc gttcggattg acgttcttca gatcgaagat
      721 gagctgcgcc aggtcttcga tcgagtagat gtcatggtgc ggcggcggtg aaatgaggcc
      781 gacgcccggc gtcgagtgac gcaccttggc gatcgtcgcg tccaccttgt ggccgggaag
      841 ctggccgccc tcgccgggct tggcgccctg tgccatcttg atctgcatga cgtccgaatt
      901 gacgaggtac tccgtcgtca cgccgaaccg ccccgaggcc acctgcttga tcgccgagcg
      961 catggaatcg ccgttgggca gcggcttgta gcggtcggcc tcctcgccgc cttcgccggt
     1021 attcgacttc ccgccgatac ggttcatggc gatggcgagc gtcgtgtgcg cctcgcggct
     1081 gatggagccg aagctcatgg cacccgtcga gaagcgcttc acgatgctgg ccgcgggctc
     1141 gacctcttcg agcggcaccg gcttgcggcc gagctcggcg gcagacttga tgcggaacag
     1201 cccgcgtatc gtcatgagct gttcggactg ctcgttgatc tgctttgcat aggagcggta
     1261 cttctccggc aggttgccgc gcaccgcgtg ctgcaggtcg gcgacgaccg tcggccgcca
     1321 cacgtgcgcc tcgccacgga cgcggtaggc atactcgccg ccgacatcga gcgcattctg
     1381 caaggtcggc acgtcaccga aagcctgccg gtgccgctcg accgtttccc gtgcgatctg
     1441 gcgcaggccg acgccttcga tcgtggtgtg cgtgccagtg aagaactttt tcacgaagct
     1501 cgacttgaga cccaccgcat cgaaaatctg ggcgccgcag taggactggt aagtcgagat
     1561 gcccatcttc gacatgacct tcagcaggcc cttgtcgatc gctttgatat agcgcttgac
     1621 cgcctccttg ggcgacaggt cctcgccgag ttcgctcgca agctgctcga tggtgtcgaa
     1681 ggcgaggtaa gggttgatcg cctccgcacc gtagccggcc agcaccgcat actggtggac
     1741 ctcgtgcgct tcgcccgttt cgaccacgag gccaacggac gtccgcagac cttgccggat
     1801 caggtgatgg tgcacggcag aggtggccag cagggacggc acgggaatgc ggtccggccc
     1861 ggtcgcgcga tccgacagga tgatgatgtt gtaatcgccg gaacggaccg cttcctcggc
     1921 ctcggcacag atcacgtcga gcgcagggcc catgccggca gcgccttgct cctgcggata
     1981 cgtgatgtcg atggtgatgg acgtgaaatg gttgtccggc acgtcgccga tctggcgaat
     2041 gcgctccagc tcgtcgttcg tgaggatcgg ctggcccacc tcgagccgct tcagcttcga
     2101 ggtgccctcc aggtcgagca ggttcgggcg cggcccaatg aacgagacga ggctcatgac
     2161 gagctcctcg cggatcgagt cgatcggcgg gttcgtcacc tgcgcgaagt tctgcttgaa
     2221 gtaggtgtgc agcagcttgg gcttgttgga cagcgccgag atcggcgtat ccgtgcccat
     2281 cgagccgacg gcttcctggc cggtctgggc catgggggcc atcaggaact tgaggctttc
     2341 ctgcgtgtag ccgaacgcct gctgcaaatc gagcaggtcg atgttggact tccgcctctt
     2401 cggcatcttc ggctgcggca ggtcgctgac ctggatctgt gtgcgccgca gccattgctc
     2461 ataaggattg cgagcagcaa gctcggactt cagctcgtcg tcggaaataa tgcggccctg
     2521 ctccagatcg atgagcagca tcttgcccgg ctgcagccgc cacttggcga tgatctggtc
     2581 ctcggggatc ggcagcacgc cggactcgga cgacatgatc acccggtcgt ccttggtgac
     2641 aaggtagcgc gcaggtctca gcccgttgcg gtcgagcgtg gcgccgatct gccggccatc
     2701 ggtgaaggcc atggcggcgg ggccgtccca cggctccatg aggcaggcgt ggtactcgta
     2761 gaaggcgcgg cggcgcgaat ccatcagcgg attgccggcc cacgcctccg ggatcagcat
     2821 catggcggcg tgggcgaggc tgtagccacc catcaccagg aattcgagcg cgttgtcgaa
     2881 gcaggccgtg tccgactggc cctcgtagga aatcggccag agcttgctga tatcgttgcc
     2941 gaacagcggc gaggacacgg aggcctgccg cgcggccatc cagttcacgt tgccgcgcaa
     3001 cgtgttgatc tcgccgttgt gcgcgaccat gcggtagggg tgcgccagct tccaggatgg
     3061 gaaggtgttg gtcgagaacc gctggtggac gagggccagg gccgacgcca tccgcctgtc
     3121 cgacaggtcc ttgtagtagg ccttgagctg ataggacagg aacatgccct tgtagacgag
     3181 ggtgcggctc gacagcgaca cggtgtaatg gccgatgtcg cgggccttgt aagcatcgta
     3241 gatcgcgttc gagacgacct tgcggacaat gtacagccgc cgctcgaagg catcgtcgtc
     3301 cctgattgcc ttcggccgcg caatgaatac ctgacggtgc acgggttccg tcgctttcac
     3361 gccgtccgac aggcaggagt tgtcgatggg cacgtcgcgc cagccaatga gctgaaggcc
     3421 ttcctcgcgt acaatgcgcg cccacacggt ctcgcagtgg cggcgcagcc tctcatcgcg
     3481 cggcatgaag atgtgcccga cggcataatg cccgggcggc ggcagcttga agccgagctt
     3541 tgcgcactcc tcggcgagga acgcgtgcgg aatctggatc agaatgcccg cgccatcacc
     3601 cgcgatggga tcggcgccca cggccccgcg gtgctcgagg ttctccagga tgctgagcgc
     3661 atcctcgatg atcccatgac tggcgcggga tttgagatcg acgatgaacc cgaccccgca
     3721 ggcatcgtgc tcgcgtgccg gatcgaacag acctttcggg gcctcgtcgt catcgaacct
     3781 gtcgttcaac gcgtcaaggt tgaagcggct ctcggtcatg ggtcggcctc tcaaccgggg
     3841 accccccggg gctcgagttt atcacaaggg actcgtcgat tgtgtgacgg ctcggggctg
     3901 gattgcgggg gcacagacgg cccgctcgtc atctgccgga aatcgagacg ctcagtctcg
     3961 cgtacgggtc cgttttcggg ttcccgtggc ggtcgagatg cgttttcggt ttgcgaccgc
     4021 gcgcacggtg ccggactgcg tccgcaccat tgccatcgcc tgatcgaacc gaaccgtaag
     4081 cactgtcgcc tctagcgggg tcttgggcca gcttctcata aaggacagga ttgctgacct
     4141 ttcgcaacac ataagcagat ttcagattgc cgcacaagcg cccctttcgg agcatcgcca
     4201 attttctgca tgcgacgtgc atgcagcgcc tgaagctgct ttcgctccca aaccaggatg
     4261 acccgggtct ggttcttgcg tcaaaagtcc cggctggacg ctgcttgccg atcgcgttcg
     4321 ttgcgacgta accgccagtt ctcgtggcca ccagaaacgg gaagcgggcg cttccggaga
     4381 agcacccgct ccctttgcat cagaccgtgt ttgggatcag gccttgctta cgtcggcctc
     4441 gatggcccgc tcgggcgtcc cgatcggaat gacccgcggg cgcatctgct cgggcaggtt
     4501 acgcacgagc tcgatgtgca gcagcccgtc ggtcaggctc gctccggtga cctcgacgta
     4561 atcggcgagc tggaagcggc gctcgaaact gcgctgcgcg atgccgcggt gcaggtactc
     4621 gcgctgtttc tcctcgggct tcttctcgcc gcggatcgtc aaggcggcgt ctttcacctc
     4681 gacattgatc tcgtcggagc ggaagcctgc gacggccatc gtgatccggt attcgttctc
     4741 gcccgtccgc tcgatattgt aagggggata gctgacggcc tcgtcgctga agcccgacac
     4801 gctgtccaaa agctggacga gacgatcaaa gccgatggtg gaacggtaaa ggggagcaaa
     4861 gtcgagtcgc atcacaagtc ctcctgacaa gcgacatgtg gtctgcctgg ccaagtggcc
     4921 caggcgctga agcggtgacc atcctcgtag gcatggacac cacggatgac ttggtgcgtg
     4981 ccctccgact ttcaagccct tcgcactcga agctgccgtg gcgctgctaa aggtaaacaa
     5041 cgccttcatc tcccgttcat cttcgcgccg cgatgctgcg ctccagtggg ggtctcacgg
     5101 agtttccggg aggatcagtg atgtccttgc gattgctgac cgccggcctt gtcgccgcgt
     5161 tacctttggc agctattcca gcagcttcgg cggccgacct cggtccgccg tccgattacg
     5221 gatactcgga atctccctac gacgatccga gatatgcgga tatctaccgt gaccccaagc
     5281 cgccctacgc gccgccattt gcgaagccgt gtgccgagcg tgactacggt tgctacgacg
     5341 aggggtatga cccaccggct cacggtaagt ggcgcggcga gtacctcgag ccattaccgc
     5401 ctccgccgga attcgatgag taccgcccgc ggaaggccta tctgcccccg gcgtgcctgc
     5461 cgcgacacga gatccggcgc gcgctgatcg aggacggctg gtccgacctg cacgacttcg
     5521 agttcgggaa acggaaggcc tttctcactg cgcggcggcc gaacggctcg ctttaccggc
     5581 tcgaggtcga ccgctgtgat ggcgaggtgg tgcgcgcgga gcgcgtcgag catgcccatg
     5641 cgcattcctg gtcatcgcgg cggaaaggtt accccacctt ctgaaccggc ggctacgaag
     5701 cgcctccggc agtgcttgga ggcgcgtttg ccgctggtcg aggattgccg ccctgcgatt
     5761 gaacttgcct tcttgccgct cattggtgaa gcctagcgcg atcgggaaat tcgcctacgc
     5821 tcgcggcagg acgcgggcga atttctcgat ccgactgggc cgccaactct ggcgaagatc
     5881 ggaatcgggg ctctgggaag gcagccgaac ggcatggaac tggtagccat cgcaagaaat
     5941 cccgtgccca gcggggcgat cgttggagcc tacgaaggct acgacggcaa tcccatgcgc
     6001 ttcgcccgct gggagcccac gcgggggcag gcgcgcggca cggtctcggt attcaccgga
     6061 cgcagcgagt tcatcgagaa gtatttcgag gtcgtcgccg acctgcgccg gcgcggcttc
     6121 gccgtgaccg tcatggactg gcgcgggcag gggggctcct accggccgct gcacaatcag
     6181 cgcaaggggt gggtgcgcag cttcgccgag tacgacaagg acctcgcctg cttcatggag
     6241 caggtggtgc agccacaatg cccggcaccg tactatgcct tggcgcattc gatgggcggc
     6301 cacatcctgc tcagggtcgc cagccaaccc ggctgtccct tcgagcggat cacggtgctg
     6361 gcgccgatgc tgcgctttca cgacagcaag gtcggcacct cgcagcgtct tgcccgcatc
     6421 tatgccgccc tgggcacgat ttgcggcttc ggccgggcct acgtccgcgg tggctctgat
     6481 gaccccgccg atccggtagt tttcgagggc aatccgctca cctccgacga ggcccgcttc
     6541 gcacgcaatc gcgcgctgat cgaagcggcc cctcaactcc tcctcggcgc gccgaccatc
     6601 ggctggctca acgccgccta ccgctcgatg gacatgctca acgatccggc ttacgccgag
     6661 cgcattaggg tgccgctcat gatagggatc gccggccggg atgtgatcgt cgatccgcgc
     6721 gccacggagg cgtttgcgaa cagggtgaag ctgtgcacca cggtcatgct tccgttggcc
     6781 cggcatgaaa tcctgcagga agccgacgac atccgcggcc gcttctgggc tgcgttcgac
     6841 gcctacttcg gcgtcgagcc ggtcgcttga gcgtgcgagg tcaggacatg agtgatgagg
     6901 tcaggcgagc agtgacaggg cttcctcgtg gactcgggga tcgccggccg ccacgatgcg
     6961 tccgccgctg gccgccgatc caccctccca ggtggtgatg cgcccaccgg cacgctcgat
     7021 gatcggaatg agggccgcca cgtcgtagga cttgaggccg gtctcgacga cgatgtcgat
     7081 cattccggcc gcgagcaggc agtaggcata gcagtcgccg ccgtagcgca cggagcggac
     7141 ccgctccttg atcgccttga gcccccgctg atccttgggc gacgagaaca agtgtgggtc
     7201 ggtggtggtg aacatcgcct cggcgaggga agggcagggc cgggtcttga tccgccgctc
     7261 cttcccgtcg ggtccgcgga aatggctgga gcgctgcccc gaccagaagc gctcgcgcgt
     7321 gtagggctgg tccatcatgc cgatgaccgg cgcactcccg tccatcagcg cgatgagcgt
     7381 gccccacagc ggcatgccga gaatgaaggc gcgggtgccg tcgatgggat cgacaatcca
     7441 gcgcatccgc gcatccgggt tgcgctgccc gtactcctcg ccttccagtc catggtcggg
     7501 gaagcgcctg gcaagttccc tgctgatggc gcgctccgcg gcccggtctg cgaccgtcac
     7561 cggatcgaag ccgccagcgg ccgccttgtt ctcgatcgcg atgcgacggc ggaagtgcgg
     7621 gaggatggct gcgccggcga gatcggcgaa atcgtgtgcc gtcgagatgt agtctgcaat
     7681 ctctgatcga gcgctcatga tcgttgcccc cgctagccgt acctcgagat cgctgcttgc
     7741 tagccgtcaa aacagccgtg tgcaatcggc tcgaggtcga aaaacggagg tattgccgat
     7801 tgcgtgtgtt gccggcgggt tacaatcgca agaatatctt cgtgcttttc ggccggacat
     7861 cttgagacga ctcgtgaatg tcagcatacc gttgatgtga gcctgcggcg gatttccgca
     7921 acgctcacgc ccaccttggg cgattcctcc ctagacttgg gccgttccga aatgagcggc
     7981 cttctttttt gtcaggcctt gacgagatct ccggcattcg ctaggccgac agaggtacac
     8041 gcctatgtcg ttgctgctct gcaaaaaacg cagttgtttt ttgtgcgctg cacctctagt
     8101 tattgcggtg cggcgggcga gcacttcgct cgtctttcgc agccctcctt gggcgtttcc
     8161 tccctagact tgggccgttc ggaaccccgg acggcctctt tttttgcaag tcgggacggc
     8221 tgaggaaggc tttgatcctg tatcggaatt gtcagggcct ggggcgttcc atcaattgcg
     8281 acgtttcgtt ccgttcttgg cacttgaaac gagttaaccc acaccacata cttatgcgcg
     8341 tgagttcggt gcttccaccg ggctcacagc cctcctttgg gcgtttcctc cctagacttg
     8401 ggccgttcgg taacccggac ggcctctttt tttgcgcgcg ctgtgagccc gggtcgccta
     8461 ttcggctgcg gcccgcggcg cgatgatggg cggcagctcc gagaagatgc gcgcgatcat
     8521 ctggacgaga tcgtgtctga gccggccaaa gttcgccgtt ctcgtcagcc gactctcgtt
     8581 caggtagaga ccccggttga tctcgatctg caaagcgtgc acgcccttga atggctgccc
     8641 gtaatgctcg gtgatgaatc cgccggcata gggccggttg agctgcactt gatagccgcg
     8701 ctgtgtgatc acctcgcgga acaggcgcgt cacctttgca tcacatgaag cgccgaagcg
     8761 gtcgccgatc acgaagtcgg ggcgcgatcc gctgggctgg tggatcgagg acgacggcat
     8821 cgaatggcaa tcgacgagaa tggcgatgcc gaagcggcgc ctggtccgct ccaggagctg
     8881 tgcaacccgg gcatgaaacg gcttatagag cctctcgatg cgctcgatgg ccgccgataa
     8941 cggcagcggc tcgtcgtaga tctcctcgcc gtcggcaacg atccgggcca gggtgccgag
     9001 cccgccgacg acccgcatcg tctgcgtgtt ggcgaaatgc ggcaggggct cctcgaagag
     9061 ctccgggtca agctcgtaag gctcgcgatt gacgtcgagg taagcgcgcg ggaaccgggc
     9121 cgcaatcagc ggcgccccaa gagtggcaac gtcgccgaac agctcgtcga cgaaacagtc
     9181 ttcggacttg cgcagggtgt gcgcgtcgag gcgcgagcgt tccaacagga cgctggggta
     9241 gacgttgccc gagtggggtg agcagaaaac gaacggcgtc gtctgctcga ccggctcgcg
     9301 gacgacgaac ggcggtgtca gctccgccag gatagacgct ctttcgcgct gggacatggg
     9361 tccatgaagt gatcagagtt gaggctcact gtgccgcaag ccggggcgca gtgtctatga
     9421 ggcaagtagc attgccccca tcggcagtgc ggctccgtag accctcggcc cggctctgcc
     9481 tggcgtctca tcggttggtg tgaagtgtgg tcggttcatt cctcgtttac ccaaatcgcg
     9541 ccagattccg ctggcacgtg gcctttgacg cccatattct gcgctcctta gcaaagcata
     9601 acgcgtaacc atgccagcta aaccttcgcg tctgccgatc gtacgcatcc tgctcgccga
     9661 ggacgacgag tcgatgcgcg gctttctcga gcgggcgctg acgaaggccg gctacgacgt
     9721 catctcgttc gccaatggag ctgatgcgta tcagcggctg aaggaggagc cgttcacgct
     9781 gctgctgacg gacatcgtga tgccggtgat ggacggcatc gagctggcgc gccgcgcgag
     9841 tgagctcgat ccggagctga agatcatgtt catcaccggg ttcgcggccg tgacgctgaa
     9901 cacggacccg ggcgcgttca aggatacgcg tgtcctgtcg aagccattcc acctcaaaga
     9961 cctcgtcaga gaggtggacc ggctgcttgc ggcgtagggt tcgccaaggc agcggaagcg
    10021 ctggacgaac gcttccgggt gggcaatcag gcccgatcgc tcagcatttg ctgagagagg
    10081 ccgacacctc gcggatgtaa tcggcccggg ccggcgtcac cgtgaccgac tgtttgcagc
    10141 ccgagccaac gaccgtcatc ttcgccgtga agaactggcc gctcagcacg acgtcgaagc
    10201 ctccttcctt cacaacgccg gccaccgtgc ctttgaggcc gacgtagatg ttgtcctgcc
    10261 agcgcccgac aaccttatcc ccgcgcaact cgagatgggt cgccgcgtcg agcttgtagt
    10321 tggcgctctt gcagcgcagg ttctgctgaa gccgcgtgac actgccttgg gggatgtagg
    10381 tcacaacgca tttgaagctc tcggccgg
//
LOCUS       DASVDU010000294         4554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_798997, whole genome shotgun sequence.
ACCESSION   DASVDU010000294 DASVDU010000000
VERSION     DASVDU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798997"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VF226_19705"
     CDS             complement(<1..579)
                     /locus_tag="VF226_19705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7076270.1"
                     /translation="MQNRWGVLALLFAVRTTMAVQFQSVAAMSPVIMREFGIGLADIG
                     LLIGLYLAPGMIIALPGGEIGRRFGDKQVVLFGLALMIGGGAMTALLPEWRWQVTGRL
                     ISGTGGVLLNVLMSKMVTDWFAGKEIATAMGIFVNSWPIGIAFALFVLPWVAVSWGLA
                     ESYLFVLLMVLVASVLLALLYRPPPVDAVAPAS"
     gene            748..1755
                     /locus_tag="VF226_19710"
     CDS             748..1755
                     /locus_tag="VF226_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX7076271.1"
                     /translation="MSNANRQVKLARRPGLGPLTRDVFAIEDGPIPEPGPGEFRVKIE
                     YVSLDPAMRGWVAEGRSYIEPVPVGAVMRGYAVGHVEASNHPDFKPGDAVQGLFGVQQ
                     YALSKGDRVVKVDTSLAPLQRWIGGLGMPGWTAYFGLLEVGQPKPGETVVVSAASGAV
                     GSVVAQIAKLKGCRAVGIAGGPQKCQYLIDELGLDAAVDYKAGNLAADLKAACPQGVD
                     VYFENVGGEILDAVLPLMNTFGRIPLCGLISAYNATTPPPGPRNLRYVLTQRLKMQGL
                     IVFDWADRIHEAIRDLGKWHAEGKLKIREDVREGGLDAFPDVLNLLYTGGNQGKLVLK
                     V"
     gene            1760..2347
                     /locus_tag="VF226_19715"
     CDS             1760..2347
                     /locus_tag="VF226_19715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7076272.1"
                     /translation="MRGARTIYFVRHGETDWNAARRLQGQTDICLNDVGRRQAARNGH
                     VLKKLVSEIAAFDFVASPLARTRETMEILREAIGLDRPGYRTDSRLMEVNFGHWEGLT
                     WAELPDRDPEGYQARLADPFGWRPRNGESYRDLMERVAGWLESLEGDAVVVSHGGVSR
                     VLRGHLLGIDPREVPYLDVPQDKVLVLRPGHMAWH"
     gene            2924..4060
                     /locus_tag="VF226_19720"
     CDS             2924..4060
                     /locus_tag="VF226_19720"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HEX7076273.1"
                     /translation="MGLLLSAAQRFVTPQLIGRMATALGIDPKLAESAASAGIPAILA
                     GLSQIAGSTSGTQRLAGATAKVPAGMLDDIGSRLSNPDGIIEQGQSLLSSLLGSGASN
                     MLMSSLARYVGSTEGSMRSLVGLLVPVIMGVLGRHQQTMGGDASGLAQQLRSEASDFK
                     AAMPSGLSSMLDSSGFFDQVGAPQAAAAGSGATREDFREGTAGERAARPTPTPTPRAQ
                     PARSDWSWAYWALPLAALIGGLIGYMLGGDQRVGDMTTALRTPDISNARPMNSYMRQP
                     VFSQAGEPLGTVEDILIARDGQVMAVLSTAQPLGLGEKRIAVPMPNIEIATRDGSTQL
                     VFKGSRDTLADFPAVDGTTGSSGVTGVAPTPPSTPGTTTTQPQQ"
BASE COUNT          890 a         1432 c         1456 g          776 t
ORIGIN      
        1 cgacgcaggc gcaaccgcat ctaccggagg tgggcgatag agcagcgcca gcagtacaga
       61 ggcgacgagc accatcaaca ggacgaagag atagctctca gcaagccccc agctcacggc
      121 cacccacggc agcacgaaca gcgcgaacgc aattccgatc ggccaggagt tgacgaatat
      181 gcccatggcc gttgcgatct ccttcccggc aaaccagtcg gtgaccatct tcgacatcag
      241 gacgttgagc agcacgccgc ccgttccgga gatcagccgg ccggtgacct gccagcgcca
      301 ctcgggcagc agcgcggtca tcgccccgcc gccgatcatc agcgcgagac cgaacaggac
      361 gacctgcttg tcaccgaacc gccgcccgat ttccccgccg ggaagtgcga tgatcatgcc
      421 aggagccaaa tagaggccga tcagcagccc gatgtctgcg agcccgatgc cgaattcgcg
      481 catgatgacg ggcgacatgg ccgcgacgga ttggaactgg acggccatgg tcgtgcgcac
      541 cgcgaacagg agcgcaagca cgccccaacg gttctgcatg atcgcctccc ccgaattgcg
      601 tcgagagagg ctaaccgcct gatactttca gcacaaggag tacctggcgg acgaactcag
      661 tgttttacag gcaggatcga gcagaagcgt gcaccaatcg aaacccagcc tatattgccc
      721 ccagcacgat ggaattggga gttgccgatg tccaacgcca atcgccaggt caagctcgca
      781 cgccgccccg gtctagggcc gctgacgcgg gatgtcttcg ccatcgagga tgggccaatt
      841 ccggaaccag gccccggcga gtttcgggtg aaaatcgaat atgtctctct cgacccggcg
      901 atgcgcgggt gggtcgccga agggcgctcc tacatcgagc cggtgccagt cggcgcggtg
      961 atgcgcggct acgccgtggg gcacgtcgag gcatcgaacc acccggactt caagccgggc
     1021 gatgccgtcc agggattgtt cggcgtccag cagtatgccc tctcgaaggg cgatcgggtg
     1081 gtcaaggtcg acaccagcct cgctccattg cagcgctgga tcggcggact ggggatgcct
     1141 ggctggactg cgtatttcgg cctgctcgag gtcggccagc cgaagccggg tgaaaccgtc
     1201 gtggtatcgg ccgcgtcggg cgccgtcggc tcggttgttg cgcaaatcgc gaagctcaaa
     1261 ggttgccgtg ccgtcggcat cgcaggcggg ccgcagaagt gccaatatct gatcgacgaa
     1321 ctcgggctcg atgctgccgt tgactacaag gcgggcaatc tggcggccga cctcaaggcg
     1381 gcgtgcccgc agggcgtcga tgtctacttc gagaacgtcg gcggagagat tctcgatgcc
     1441 gtcctgccgc tgatgaacac ctttggccgc atcccgctct gcggactgat ctccgcgtac
     1501 aacgcgacga cgccaccgcc tggcccgcgg aacctcagat atgtgctgac gcagcgcctg
     1561 aagatgcaag gcctcatcgt tttcgactgg gccgaccgca tccatgaggc catccgcgac
     1621 ctcggcaagt ggcatgcgga aggcaagctc aagatccgcg aggacgtgcg cgagggcggt
     1681 ctcgacgcat tcccggacgt gctcaacctg ctctacacgg gcgggaacca aggcaagctg
     1741 gttctgaagg tgtgagggaa tgcggggggc gaggacaatc tatttcgtgc gccacggcga
     1801 gacggactgg aacgcagcgc ggcggctgca aggccagacc gacatctgcc tgaatgacgt
     1861 cggccgccgg caggcggcgc ggaacggcca tgtgctgaag aagctcgttt ccgagatcgc
     1921 agcattcgat ttcgtcgcca gcccgctggc ccgcacgcga gagacgatgg agatcctgcg
     1981 cgaggcgatc ggcctcgacc ggccaggcta ccggaccgat tcgcgcctca tggaggtgaa
     2041 cttcggccac tgggaaggcc tcacctgggc cgagctgccg gatcgcgatc cggaaggcta
     2101 ccaggcccgt ctcgccgatc ccttcggctg gcgcccgcga aacggtgaaa gctatcgcga
     2161 cctgatggag cgcgttgcag gctggctcga aagtctagaa ggcgacgctg tggtggtctc
     2221 gcacggcggc gtcagccgcg tcctgcgcgg tcatctgctc ggcatcgacc cacgtgaagt
     2281 gccgtatctc gacgtgcccc aggacaaggt gctggtactg aggccggggc atatggcctg
     2341 gcattagcga gcttcggccg ccctgagcgg gatcaggcgc ggccgaagac gtgccaggaa
     2401 tcgccgtcag aagcagcggc ggtccgcagg atagcagcta cccttgcgga atacggggtc
     2461 gtagcagcat ttctcgctgg cattgcatat ccagctcacc tgcgctccgt cccgggtcgg
     2521 ggcgatgcac cttttgcggg taaaagcaat cggactgtac gtgcgaacgg ggcgataaat
     2581 gccgtcagcc cgtgcttctt ggctcaaagg catggcaaac accagggcga gcgcgcaccc
     2641 gaaagccgcg atccaacact tcattctttc ctccacgaaa agtcacgcga cgcccccatt
     2701 gtcgcaaatg cgtgagacaa gcgcttgctt caacttgaag ttgccttaat gcaaggtttg
     2761 aggcactgac ccgaaacggt tcgagagctg acgcagcgcg agcgacctgc atctcatcaa
     2821 gcgctaagtt gccggaactc ttggggtctc atggaacctg ctgattagcg ccgagttgcc
     2881 cgcatgacgg ggaagtggcg gtgcaattta caaagggtgc ctcatgggac tccttctttc
     2941 agcggctcaa cggttcgtca ccccgcaact catcgggcgg atggccacag cgttaggtat
     3001 tgatccgaag ctggcggaaa gcgccgcctc cgccggcatc ccagccattc tcgccggctt
     3061 gtcgcaaatc gccggcagca cgagcggcac gcagcgactg gcaggcgcga ctgcgaaagt
     3121 gccggcagga atgctcgacg acattggctc tcgcctgtcg aaccccgacg ggataatcga
     3181 gcagggtcag tcgttgctct cctcgctgct cggtagcggc gcatcgaaca tgttgatgtc
     3241 gagcctcgcc cgatacgtcg gcagtactga aggatccatg cgctccctcg tcggactgct
     3301 ggtgccagtt atcatggggg tgctcgggcg ccatcagcag acaatgggcg gcgatgcctc
     3361 agggttggcg caacagttgc gctccgaagc cagtgacttc aaagcggcca tgccctccgg
     3421 gctttcgagc atgctcgact ccagcggctt cttcgatcag gtcggcgcgc cgcaggcggc
     3481 tgctgccggt tcgggagcca cccgagaaga tttcagagaa ggcactgccg gagaacgggc
     3541 ggcgcggcca acacctaccc caacgccgag agcgcaacct gcgcgcagcg attggagctg
     3601 ggcttattgg gctttgccac tggccgccct catcggtggt ctcatcgggt acatgctcgg
     3661 cggcgaccag cgggtaggcg atatgacgac tgctctccgg acgcccgaca tatcgaacgc
     3721 caggccgatg aacagctaca tgcgtcagcc ggtcttcagt caggcgggcg agccgctcgg
     3781 cacggtcgag gacattctca ttgctcggga tgggcaggtg atggctgttc tgagcaccgc
     3841 gcaaccgctc ggattgggcg agaagcgcat cgccgtgccg atgcccaaca tcgagatcgc
     3901 cacgcgtgat ggaagcacgc agctcgtctt caaaggttcg agggacactc tggccgactt
     3961 cccggccgtc gacggaacca cggggagcag tggtgtaact ggtgtcgcgc cgactccacc
     4021 cagcaccccg ggcaccacga cgacgcagcc tcagcagtag gggcagggca gccggtagtc
     4081 ggccgagccc aggcaatcaa taacggggca aaagctcggc actgaatatt tctaaaccgc
     4141 ctcccgctat gggtgcctga ggcggttcat ttttggttcg cgagaccgcc ggcggtctcg
     4201 gtttcgtgaa ttcgaacagc ggtaggtggg accgcgctct gaaaatgtcg agagccgaag
     4261 cagcggcagc ttcggctctc tcatgcgtat gacgtgacaa gcaaaacggt tgctcaggcc
     4321 gtcctgcggc cacggcgacc agctcgcgcg tgcttcgagc cggattcgct gcgacgaaga
     4381 ccttgggcgt gcgatgcccg ccgtcctcag ccgcccacca atacttgata ctggtgcacc
     4441 atcgctccgt tagctcccgc gcgctacggg accaacgaat cacaatagtg caatccctgc
     4501 ttaagcctgt cgtcgttcgc tgtcatcaga agttcgcggg ttcgcctaaa ggca
//
LOCUS       DASVDU010000295        12027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_273552, whole genome shotgun sequence.
ACCESSION   DASVDU010000295 DASVDU010000000
VERSION     DASVDU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273552"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VF226_19725"
     CDS             complement(<1..198)
                     /locus_tag="VF226_19725"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517228.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HEX7076274.1"
                     /translation="MSPLDDKLGPIFTDVLRRNAGEVEFHQAVREVLESIGLVIAKRP
                     EYADQALIERICEPERQVIFRV"
     gene            complement(465..1364)
                     /locus_tag="VF226_19730"
     CDS             complement(465..1364)
                     /locus_tag="VF226_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076275.1"
                     /translation="MAIAEETPQITYKVPKKRHPLLQFAFTQPLGAAGLVFIIALFIA
                     GIFAEYVAPYDPLALDYGAMLAPPSLDHPMGTDAFGRDVFSRIIYGARTALAVGFLSS
                     LLGSGIGAVIGVVSAYFGGRIDMLIQRFMDILLSFPIIVLALAVVAVLGKWPVGGLDL
                     NLIVAIAIPMVPKVARVVRSAALAIREMPYVDAARAAGYGHARIIFRHIAPNVVAPFL
                     IMFTAFIAQAILLEASLSFLGLGVTEPTAAWGLMLSGSSEEFYQQAPWMIIFPGLAIS
                     FAVFAFNLFGDSLRDWLDPKIKL"
     gene            complement(1366..2319)
                     /locus_tag="VF226_19735"
     CDS             complement(1366..2319)
                     /locus_tag="VF226_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076276.1"
                     /translation="MLRYTINRLLLMIPTLLGVAVLVFLLLRMMPGDIVELRFLMEGG
                     QVSPEVLAQERARLGLDQPLWQQFIDWMTGLAVGDFGVSMWTGRPVWEEISSRLELSM
                     QVAIMATILATLIAIPLGTLAALFKDTWIDRIIQVFAIAGLAVPSFWLGMLMILGLLL
                     TFNWIPPLTFTPIYEDPWTNLSQLIWPAMAVGYRYSAVATRMTRSTILEVLQEDYIRT
                     ARAKGVFERLVVARHAMRNAMLPVVTVIGLEFAFLIGGLVVTEQVFNLNGIGKLFVET
                     VTRADYTMVQALVMLVATFFIVINFLVDLLYAALDPRIRYR"
     gene            complement(2408..3991)
                     /locus_tag="VF226_19740"
     CDS             complement(2408..3991)
                     /locus_tag="VF226_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076277.1"
                     /translation="MPRKLLLSVALTTAVALLPAVADAQTPKTGGELRFAVSAEPPDY
                     DCHAATSFAFIHPVRPHYNTLLKFDPENYPKVVGDLAESWEQSADGLTYTFKLKKNVK
                     FHDGTPFTSEDVKATYERIRNPQGGARSIRKAAYADIDSIETPDEYTVVFKLKERNAS
                     MLTNFASPWDCIYSAAKLKEDPRFPERNVLGTGPFKFVEHAAGSHWIGERFEDYHVEG
                     RPYLDSYRAIFITNTAARVNALQAGEVLAEFRGHSPADRDKLVSALGDKIEVHESPWI
                     CSLVVTFNTEKKPFDDARVRRALSLAVDRWQGAEALSKIALVRHVGGVLRPGYELATP
                     PEELEKLPGFSRDIKASRAEAKKLLKEAGVSDLKFKLMNRNVPMPYTPVGVFLIDQWR
                     QIGVSVEHDQPETKAYIANLRGGNYEAGLDFNCDAVDDPNLQLAKYISSDRSPINYSR
                     AIDRKLDELYDAQKGELDVEKRKQILREFEKHALTEAYTIPTIWWHRIVVTWKQLKGW
                     HMSPSHYLNQDLQDVWLDQ"
     gene            complement(4034..6121)
                     /locus_tag="VF226_19745"
     CDS             complement(4034..6121)
                     /locus_tag="VF226_19745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076278.1"
                     /translation="MTAQLVAEAVSDRDAAPSPASAPWLLEVDNLHVHFVTSAGVVRA
                     VEGLSYKVKPGEVMALVGESGCGKSVSAMTVMRLLAKPAGRIVAGRIMFQGRDLLKLS
                     EEEMRAIRGREISMIFQEPMTSLNPVLTIGFQIMEPLMIHLGMSEEQARARAAELMRQ
                     VGIPDGERRLDQYPHQFSGGMRQRVMIAIGLACNPKLIIADEPTTALDVTIQAQILQL
                     MKDLSRELGIAMVIITHNLGVVARYADRVNVMYAARMVEQGTADDVFERPRHPYTYGL
                     LRSVPRLDRPREAKLETIEGLPPNLVSPPEGCRFAPRCAAAIDACKAKQPPLIEVEDG
                     HFSACIRATELAEKGPQAIGLSTAIGAAKQDKDLGDAEPLVRIERLTKHFVVSQGSKL
                     FGREPSVVRAVNDVSFSIAPGQTLGLVGESGCGKTTVGRLVLKLEEATSGAILFEGKD
                     LTTAAHDTLRDVRRRIQVIFQDPYSSLNPRMTIGQIISEPLLVYRMVPSRKAARERVE
                     NLLTQVGLYPYMADRYPHELSGGQRQRVGIARALAMEPKFIVCDEPVSALDVSIQAQV
                     INLLEELQEKYGLTYLFIAHDLAVVRHISHRIVVMYLGRVMEIADRDSLYENPLHPYT
                     QALLEAVPVPDPKAERARKHTILSGEVPSPLNPPSGCVFRTRCPRATAECKVAIPELR
                     KFGKDHYAACINI"
     gene            complement(6582..6992)
                     /locus_tag="VF226_19750"
     CDS             complement(6582..6992)
                     /locus_tag="VF226_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076279.1"
                     /translation="MKRTFVATLLCGAMMVQPALAGVAMPAPVTAVPASGAVVPAVAG
                     DQYAGGAETAKVIEVRYWRHRHHHHHHHHGGAGAFGAGLAVGIIGALIAKGISESSAR
                     ERFDRCARDFRSFDYETGTIITRSGEERLCPYLR"
     gene            complement(7408..7701)
                     /locus_tag="VF226_19755"
     CDS             complement(7408..7701)
                     /locus_tag="VF226_19755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076280.1"
                     /translation="MNDREDEATRRLEDLEARIEKARKDLEQHDVITGELRREWDDML
                     RRHAELRRRLQSGEAKKSEAAATLNEDIDVLRHAFFRWASQVEERFKGPPDVQ"
     gene            7866..8804
                     /locus_tag="VF226_19760"
     CDS             7866..8804
                     /locus_tag="VF226_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX7076281.1"
                     /translation="MISLDDVIAARNRIGPYIRSTPMLRAAALKQALPYQNVSLKLEC
                     LQVTGSFKARGAMNRVLGAAPEELKAGLVTASGGNHGLAVARTAHVTGVPATVFLPSN
                     VSPAKVEKLKGWGARIEIGGKVFDEANEAALAHASQSGALYVHPYADPLVVAGQGTLG
                     LDIVDSMPDLDAILIAIGGGGLIAGMATAIKSLRPSVRIIGVEPQGAPTLQASLAAGR
                     VVMLPEITTSVATMAAQRTDERVFEIARRHVAEVVLVSDDEMRAAARWLWFEMGIAAD
                     LSGAAAIAALQSGRLDLPPEANICALVCGAGPDGIA"
     gene            complement(8823..9191)
                     /locus_tag="VF226_19765"
     CDS             complement(8823..9191)
                     /locus_tag="VF226_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076282.1"
                     /translation="MRTSSPGRRRLAASLIAAVSLLPLTTGSPGVAAGEGVHGCLMSE
                     ADQFFTRLAAAIGASEVEAASIDDAYIERASQPILAKCANEADPSADAGVAAFRAHMA
                     RWSYHLDSKLSSINARGAPD"
     gene            complement(9303..10550)
                     /locus_tag="VF226_19770"
     CDS             complement(9303..10550)
                     /locus_tag="VF226_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEX7076283.1"
                     /translation="MNEIGDRPDQSRASRLGGPALPTSFGEFVALMALLMALTALSID
                     IMLPALPQIGSFFGLADANDSQLVVTSYMIGFAAGQIVFGPLSDRFGRKSMLMVGLVI
                     FAVGSFAAATVDSFLLLFLARTVQGFGAASSRVVSIAVIRDLFSGWEMARVTSLVMIV
                     FITVPVLAPSLGQLLLYLGDWTLIFEFLLCVGIACLVWSGYRLPETHATNASRRRIKL
                     ATALRWTFTSLQTVGYSVAIGFVFGCLMAYINSAQQVFVDVYGLGAAFPLVFGAVAGV
                     QAIAAFTNAQLVERHGMRRVSHTALVAFCASALLLMILAPWKPPLLVFAVLVAALFFA
                     FGLIMPNFNAIAMQPMGAVAGTASSFVGFYTTAAGALLGWGIGGFFDGTIQPLATGFA
                     VLSIAAFLTVLAVEGPRNLFRGE"
     gene            11135..11914
                     /gene="bcsS"
                     /locus_tag="VF226_19775"
     CDS             11135..11914
                     /gene="bcsS"
                     /locus_tag="VF226_19775"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HEX7076284.1"
                     /translation="MVQGRWAISAVAALSGAIAFASGAYAGSLKDYSEPEPISTPKST
                     VTFSGSDWAKDSSYFYSGVIYALNRDLGRDGVVLRGFAGVPMFEYDGGAETIDGDGIQ
                     GDLMIGYMWQRPFATFSAFVGVEYQDYDLTPDDPTNKVRGSETGFKVAADLASAYESP
                     LYFNLSGSYSTAFDSYWAQGRIGYNAGRFVIGPEVIAVGSEGFDARRVGGFATLRFEL
                     SPAISSEITVYAGHQFVDDEGGESSRGGEGTYGGVGFTLVF"
BASE COUNT         2338 a         3750 c         3807 g         2132 t
ORIGIN      
        1 aacgcggaaa ataacctggc gctcaggctc gcagatccgc tcgatcaatg cttgatcggc
       61 gtattcgggg cgcttggcga tcacgaggcc gatgctttcg agcacctccc tcacagcctg
      121 gtggaactcg acctcgccgg cattgcgccg cagaacatcg gtgaagatcg gccctagctt
      181 atcgtccaaa ggagacaccg tcgtaatcct cgacaatatg cgaacgcagc aaagtggttc
      241 caggcactgc gcccgggcgc aatggaccga ctactcagtc tgcgtgcaaa cccccgacag
      301 aaaaccccga gctggtgcat cgggaagatg agcttcctta cagcaatgac cgttttttga
      361 gcaaggtggt tagtagcgca tagaacaatt cgccacggcc ccaacgaaag ttggaggccg
      421 cagcggaagg caaagtgcgc cccgtagcgt tgtcaaaatg gcagctaaag cttgatcttc
      481 gggtccagcc agtccctcag cgaatcgccg aacaggttga acgcgaacac ggcgaagctg
      541 atggcgaggc ccggaaagat gatcatccag ggcgcctgct gatagaactc ctccgagctg
      601 ccggagagca tcaggcccca tgccgccgtc ggctcggtca cgcccaggcc caggaacgag
      661 agcgaggcct cgagcaggat cgcctgggcg atgaacgccg tgaacatgat caggaacggc
      721 gccaccacgt tgggcgcgat atgccggaag atgatgcgag cgtggccgta gccggccgcg
      781 cgggctgcat ccacgtaagg catctcccgg atggcgagtg cggcggacct caccacgcgc
      841 gcgaccttcg gcaccatcgg gatggcgatg gcgacgatga ggttgagatc cagcccgccg
      901 accggccatt ttcccaggac ggcgacgacg gcgagagcca gcacgatgat ggggaaggac
      961 aacaggatgt ccatgaagcg ctggatcaac atgtcgatcc gtccgccgaa gtaagcggag
     1021 acgacgccga tcacggcgcc gatgcccgac cccagcagcg aggacaggaa gccaacggcc
     1081 aatgccgtgc gcgcgccata gatgatgcgc gagaatacgt cgcgtccaaa ggcatctgtc
     1141 cccatcggat gatccaatga cggcggtgcc agcatggcac cgtagtcgag cgccagagga
     1201 tcgtacggcg cgacgtattc ggcgaagatg ccggcgatga agagcgcgat gatgaacacg
     1261 agcccggcgg caccgagcgg ctgcgtgaag gcgaactgca gcagcggatg ccgcttcttc
     1321 ggcaccttgt aggtgatctg cggggtttct tcggcaatgg ccatatcagc ggtaccggat
     1381 gcggggatcg agtgcggcgt agagcagatc gaccaggaag ttgatgacga tgaagaacgt
     1441 cgcgaccagc atcaccaggg cctgaaccat ggtgtagtcg gcgcgcgtca ccgtctcgac
     1501 gaacagcttg ccgatgccat tgaggttgaa gacctgctcg gtgacgacca ggccaccgat
     1561 caggaacgcg aactccaggc cgatcacggt gacaaccggc agcatggcgt tgcgcatcgc
     1621 gtggcgggcc acgacgagcc gctcgaaaac gcctttggcc cgcgcggtgc ggatgtagtc
     1681 ctcctgcagc acctccagaa tcgtcgagcg ggtcatgcgc gtcgcgacgg ccgagtatcg
     1741 ataaccgacc gccatcgccg gccagatgag ctgcgaaaga ttggtccagg ggtcctcgta
     1801 gatcggcgtg aaggtcaagg gcgggatcca attgaaagtg agcaacaggc ccaggatcat
     1861 cagcatgccg agccagaagg acggtaccgc gagacccgcg attgcgaaga cctggatgat
     1921 gcggtcgatc caggtatcct tgaagagcgc ggcgagcgta cccagcggaa tggcgatcag
     1981 cgtcgccagg atggtcgcca tgatggccac ctgcatggac agctccagcc ggcttgaaat
     2041 ttcctcccag acgggacggc ccgtccacat cgagacgccg aagtcaccga cggcgaggcc
     2101 cgtcatccag tcgatgaact gctgccatag cggctggtcg agaccgaggc gagcgcgttc
     2161 ctgcgccagc acctccgggc tcacctgccc gccttccatc aggaatcgca gctcgacgat
     2221 atcgccgggc atcatgcgta gcagcagaaa gacgagaacc gccacgccca gcagggtcgg
     2281 tatcatcagg agcaggcggt tgatggtgta gcgaagcatc gcgagccgat cgaaaactca
     2341 aaggcgaaaa gatggaccgg gcagtccggc atagagccgg accgcccgct ctattgctga
     2401 ccacccgtca ctgatcgagc cagacgtcct gaaggtcctg gttcaggtaa tggcttggcg
     2461 acatgtgcca gcccttcaac tgcttccagg tcaccacgat gcggtgccac cagatggtcg
     2521 ggatcgtgta ggcctccgtc agagcgtgct tctcgaactc gcgcaggatc tgcttgcgct
     2581 tctcgacgtc gagctcgccc ttttgcgcgt cgtagagctc gtcgagcttc cggtctatgg
     2641 cgcgcgaata gttgatgggc gacctgtcgg acgagatata cttggcgagc tgcaaattcg
     2701 gatcgtcgac cgcgtcgcag ttgaagtcga ggcccgcctc gtagttaccg ccacgcagat
     2761 tggcgatata ggccttcgtc tccggctggt catgctcgac ggaaacaccg atctgccgcc
     2821 actggtcgat caggaaaaca ccgaccggcg tatagggcat cggcacgttg cggttcatga
     2881 gcttgaactt caggtcgctc acgccagcct ccttcagaag cttcttggct tctgcccggg
     2941 aggccttgat gtcacgcgag aacccgggaa gcttctccag ctcctccgga ggcgtggcca
     3001 gctcgtagcc gggccgcagc acaccaccaa cgtggcgcac aagggcgatc ttggacagag
     3061 cttcggctcc ctgccagcga tccaccgcca gagacaacgc cctgcggacg cgcgcgtcgt
     3121 cgaacggttt cttctccgtg ttgaaggtca cgacgaggct gcagatccag gggctttcgt
     3181 gcacctcgat cttgtccccc agcgcgctca caagcttatc gcgatcagcc ggcgaatggc
     3241 cgcggaactc ggcgagcact tcgcccgcct gcagcgcgtt cacgcgcgcc gcagtgttgg
     3301 tgatgaagat agcgcggtaa ctatcgagat atggccggcc ctcgacgtgg tagtcctcga
     3361 accgctcacc gatccagtgc gagccggcag catgctcgac gaacttgaag ggcccggtgc
     3421 cgagcacgtt ccgctccggg aacctcgggt cctccttcag cttcgcagcg ctatagatgc
     3481 agtcccacgg cgaagcgaaa ttcgtcagca tcgacgcgtt ccgctccttc agcttgaaaa
     3541 cgacggtgta ttcgtccggc gtttcgatgg aatcgatgtc cgcgtaagcc gccttgcgga
     3601 tcgatcttgc gccgccctgg ggattgcgga tgcgttcata cgttgccttg acgtcctccg
     3661 acgtgaatgg cgtgccgtcg tggaacttca cgttcttctt cagtttgaaa gtgtacgtta
     3721 gaccatccgc agactgctcc caggactccg caaggtcacc gacgaccttc gggtaattct
     3781 ccggatcgaa cttgagcagt gtgttgtaat gcgggcgcac cggatggatg aatgcgaacg
     3841 aggttgccgc gtggcagtcg taatccggcg gctcggcgct cacagcaaag cgcaactcac
     3901 cgccagtttt tggcgtctgc gcatcggcaa ccgccggcaa cagagcgacc gcagtagtga
     3961 gtgccacgga taataggagc tttctcggca tcagttcctc ccagccaagg tgaacgtgaa
     4021 agtcagcacc tgtttagatg ttgatgcagg cggcgtagtg atccttcccg aatttgcgca
     4081 gttccggaat ggcgacctta cattccgcgg tcgctcttgg gcaacgggtg cggaaaacgc
     4141 acccggatgg cgggttgagg gggctcggca cctcaccgct caagatcgta tgcttgcgcg
     4201 ctcgttcggc cttcggatca ggcaccggca cggcctcgag cagcgcctgc gtatagggat
     4261 ggagcgggtt ttcgtagagg gagtcgcggt ccgcgatctc catgacccgg ccaagataca
     4321 tgaccacgat ccggtgcgag atgtgccgca ccacggcaag gtcgtgagca atgaagaggt
     4381 acgtgagccc gtacttctcc tgcaactcct cgaggagatt gatgacctgt gcctggatcg
     4441 agacatcgag cgctgagacc ggctcgtcgc agacgatgaa cttcggctcc atcgccagcg
     4501 cacgcgcgat gccgacgcgc tggcgttgtc ctccggaaag ctcgtgcgga tagcggtcgg
     4561 ccatgtacgg atagagaccg acctgggtca gcagattttc gacgcgttcc cgcgcagcct
     4621 tcctgctcgg aaccatgcgg tagaccagga gcggctcgga aatgatctgg ccgatggtca
     4681 tgcgcgggtt gagcgacgaa tagggatcct ggaagatgac ctggatacgc cggcggacat
     4741 cgcgcagggt gtcgtgagcc gccgtggtca gatccttgcc ctcgaacagg atcgcgccgg
     4801 acgtggcctc ctcgagcttc agcacgaggc ggccgaccgt cgtctttccg cagcccgact
     4861 cacccacgag gcccagcgtc tgcccgggcg cgatggaaaa gctgacgtcg ttgaccgcgc
     4921 gcacgaccga aggttcccgg ccgaacagct tcgagccctg cgaaacgaca aaatgtttcg
     4981 tgaggcgctc gatgcggacc agcggctcgg catcaccaag atccttgtcc tgcttggcgg
     5041 cgccgatcgc ggtggacagc ccgatggctt gcggcccctt ctcggcgagc tcggtcgccc
     5101 ggatgcaggc ggagaaatgg ccgtcctcga cctcgatgag cggcggctgc ttagccttgc
     5161 aggcgtcgat cgcagcggcg caacgcggtg caaaccggca accctcgggt ggcgaaacga
     5221 ggttcggcgg cagcccttcg atggtctcca gcttggcttc gcgcggcctg tccagccgcg
     5281 gcaccgaacg cagcaaaccg taggtgtaag ggtgacgcgg acgctcgaag acatcgtcgg
     5341 ccgtcccctg ctccaccatg cgcgccgcgt acatcacatt cacgcgatct gcgtagcgcg
     5401 ccaccacgcc gaggttgtgc gtgatgatga ccatggcgat gccaagctcg cgcgacagat
     5461 ccttcatgag ctgcaggatc tgcgcctgga tggtcacgtc gagcgcggtg gtgggctcgt
     5521 ctgcgatgat gagcttcggg ttacaggcga gcccgatggc gatcatcacg cgctggcgca
     5581 tgccgccgga gaactggtgc gggtactggt cgagccgccg ctcgccatcg gggattccga
     5641 cctgacgcat cagctcggcc gcgcgggctc gcgcctgctc ctcgctcatg ccgagatgga
     5701 tcatcagcgg ctccatgatc tggaagccga tggtgagcac cggattgagc gaggtcatcg
     5761 gctcctggaa gatcatggag atctcccggc cgcggatcgc gcgcatctcc tcttcgctga
     5821 gcttcagcag gtcacggccc tggaacatga tccggccggc gacgatgcgt cctgccggct
     5881 tggccagcag ccgcataacg gtcatagccg aaacggattt tccgcagccg gattcgccga
     5941 ccagcgccat cacctcgccg ggcttgacct tgtaggaaag gccctccacc gcgcgcacga
     6001 ccccggccga agtgacgaag tggacgtgca gattgtccac ctccagaagc cagggtgcgg
     6061 atgcgggaga gggtgccgcg tccctgtccg acacggcctc ggcaaccagt tgtgcagtca
     6121 cggttgggcc aaacccggcc gctcagaacc gcggctcctc gagcatatgg caacgatgac
     6181 gaagccgctg gaccgacggc attgggcgat cgcgtctgta ctactggcca tcaatactct
     6241 cgacctggcg cgcacttgcg tgcccgccgt cctcccattt cgttcgcaga tcaaaggaaa
     6301 caaacagcgg cgagcagtcc cggcggacta cctgcacgcg ttacgctttg atcttgcttg
     6361 gcgattacct ccgaaggtgt ggaggtcgcc tccctgccca tgcatagcgc cctatcggcc
     6421 acacgacaac ccgttcgtcc ttaaacttga ggcggagtgt cgttgcggcc atgagaggtc
     6481 tgcagccatc gctcgcgcaa tcgatgtccg gtttcaggaa tcgatcaggc cgcagcgggg
     6541 atgcgccgcc gcggcccgat agcattgctg cggaattggg ctcaacgcag ataggggcag
     6601 agccgctctt cgccggaacg cgtgatgatc gtgccggtct cgtaatcgaa ggagcggaag
     6661 tcccttgcgc agcggtcgaa gcgctcccgc gccgaggatt cgctgatccc cttggcgatc
     6721 aacgccccga tgatcccgac cgccagcccg gcgccaaagg cgcctgcgcc accatgatga
     6781 tgatggtggt ggtggtggcg atgccgccag taacgcacct cgatcacctt ggccgtttcg
     6841 gctccgcccg cgtactgatc ccctgcaacg gccggaacga cggcacccga agccggcacc
     6901 gcagtcaccg gagcaggcat cgccacgcct gctaaagcgg gttgcaccat catggcaccg
     6961 cacagcagtg tcgcaacgaa agtcctcttc atcgaaaggc tctcctctaa gccgataagg
     7021 agcagcagag ctcgtgttgc atctatcgcc tcattccgga gctcagatgg caactttaag
     7081 tcttctcctt gcgccagcgt ttcggctccg gtctccttcg tggagaccag cgctaacacg
     7141 gcagtggttt ccggcagatg taggaaaagc caacatttct gctatggcgg tatcgttctg
     7201 gcgctggtgc aatgcgccgc tttggcgcgc tgccgcgcgg gggggaagag tttggcggac
     7261 acgggcgtcc gcgacagtcc gacccttctg caatccgtgc agcgcgcttt ccggtgagcg
     7321 ggcgtccgaa caattcggaa tatcaggccg aagcagccga agggcgaacg ccgcattatt
     7381 gtgtctgcac gtctcttgcg tctgctatca ctgcacgtcg ggaggtccct tgaagcgctc
     7441 ctcgacctgg gaagcccagc ggaagaacgc gtgccggagc acgtctatgt cttcattcag
     7501 ggtggcagcc gcctcgctct tcttcgcctc gcctgactgg agcctacggc gcaattccgc
     7561 atgcctgcgc agcatgtcgt cccactcccg ccggagctcg ccggtgatca catcgtgctg
     7621 ctcgagatct ttgcgcgcct tctcgatgcg agcctcgaga tcctccagcc ggcgagttgc
     7681 ttcatcctcc cggtcgttca tgtgtcactc cagtccgttc tttgcacccg aagtcggtaa
     7741 tttgaagacg cggcagcccg gcccgacgtt cctgagacga tgattccctc gccgacggcc
     7801 tgttgacggg ggtcgtccgg cagccccact gtgggcgccg tgcctgcaac gctggagtga
     7861 tctgcttgat ctcgctcgac gacgtcattg ctgcccgcaa ccggatcggg ccctacatcc
     7921 gcagtacgcc gatgctgcgg gccgcagccc tcaagcaggc gttgccgtac caaaacgtgt
     7981 cgctgaagct cgagtgcctg caagtcaccg gctcattcaa ggcccgaggc gccatgaacc
     8041 gggtgctcgg cgcagcgccg gaggaactga aggccgggct ggtcacggcc tccggcggca
     8101 accacgggct cgcggtggcg cgaacggcgc acgtgaccgg cgttcccgcc acggtgtttt
     8161 tgccaagcaa cgtctctccg gccaaggtcg agaagctgaa gggctggggc gctcggatcg
     8221 agatcggggg caaggtcttc gacgaggcca acgaggcagc cctggcccat gccagccaat
     8281 cgggcgcgct ttatgtgcac ccctacgccg acccgctcgt cgttgcaggc cagggcacgc
     8341 tgggactcga catcgtcgat tccatgccgg acctcgatgc gatcctgatc gccatcggcg
     8401 gcggcggcct catcgctggg atggcgacgg ccatcaaatc gcttcgacca tcggttcgta
     8461 tcatcggcgt cgagccgcaa ggcgccccga cgctccaggc cagtcttgct gcgggccgcg
     8521 tggtgatgtt gccggagatc accacgtccg ttgcaacgat ggcggcgcag cgaacggatg
     8581 agcgcgtctt cgagatcgcc cggcggcacg ttgccgaggt ggtgctggtg agcgacgacg
     8641 aaatgcgggc ggccgcacgc tggttgtggt tcgaaatggg catcgctgct gacctgtccg
     8701 gcgccgccgc gatcgcggcc ctgcaatcag gccgtctcga cctcccgccc gaagccaaca
     8761 tttgcgcgct cgtctgcggt gctgggccgg acggaatcgc ctgaccggcg tcccgtggct
     8821 cctcagtccg gggcgccccg cgcgttgatg ctggagagct tgctgtcgag atgatagctc
     8881 caccgggcca tatgcgcccg gaaggctgcg acccctgcgt cggcggacgg gtctgcctcg
     8941 ttggcgcact tggccaagat cggctgcgac gcgcgttcga tgtaggcatc gtcgatgctc
     9001 gccgcttcga cctcggatgc gccgatagcc gccgcgagcc tggtgaaaaa ttgatccgcc
     9061 tcgctcatca ggcagccatg cacaccttcg ccggcggcca cgccggggct acctgtcgtg
     9121 agcggcagca gcgaaacggc agcaatgagc gaggccgcca gtcgccggcg gcccggagag
     9181 gatgtgcgca tgatcatccc tggtctgtgc aagttccggc gtggctctaa ttggcgatta
     9241 tggctttttc ggaaatcgag gcgtgtcgtc ccgatttgcg cgcggaaatt gcatcgaaca
     9301 gattactcgc cgcgaaacag gttccgcgga ccctctacag ccaggaccgt caggaaggct
     9361 gcgatgctaa gcaccgcgaa gccggttgcc agcggctgga tcgtcccgtc gaaaaatccg
     9421 ccgatccccc atccgagcag ggccccggcc gcggtcgtat agaagcccac gaaggatgac
     9481 gccgtacccg cgacggcccc catcggctgc atggcaatgg cattgaaatt cggcatgatc
     9541 agcccgaagg caaagaacag ggccgccacc agaacggcaa agaccagcaa cggtggcttc
     9601 catggcgcca ggatcatgag cagaagcgcg cttgcgcaga aggcgaccag cgccgtgtgc
     9661 gagacccgcc gcatcccgtg ccgctcgacg agctgcgcat tggtaaaggc agcgattgcc
     9721 tgcacgcccg cgaccgcccc gaagacgagc ggaaaagctg cgccaaggcc gtagacgtcg
     9781 acgaaaacct gctgagcgct attgatgtag gccatcaggc agccgaacac gaacccgatc
     9841 gcaacggagt atccgaccgt ctgcagcgac gtgaacgtcc agcgcagcgc cgttgcgagc
     9901 ttgatccgcc gccgcgacgc gttggtcgcg tgcgtctcgg gcagccggta gcccgaccag
     9961 acgaggcagg ctatgccgac gcagagcaga aactcgaaga tcagggtcca gtcgccaagg
    10021 tagagcagca gttggccgag actgggcgcc agcactggaa cagtgatgaa gacgatcatc
    10081 acaagggagg tgacgcgcgc catctcccag ccgctgaaga ggtctcggat gacggcaatg
    10141 gagacgacac gcgaggacgc ggccccgaag ccctgcacgg tgcgtgcgag aaagagaagc
    10201 aagaagctgt cgacggtggc tgcggcaaag gagccgacgg cgaaaatgac gaggcccacc
    10261 atcagcatgg acttgcggcc aaagcggtcg gacagcggcc cgaagacgat ctgcccggca
    10321 gcaaagccga tcatgtagct cgtcacgacg agctggctgt cattcgcatc ggccagcccg
    10381 aaaaagctgc cgatctgcgg cagggccgga agcatgatat cgatcgacag cgcggtgagc
    10441 gccatcagca gggccatcag tgccacgaac tcgccgaagg acgtcggcaa cgccggtccg
    10501 cccaagcggc tcgcgcgcga ctgatctggt ctatcgccga tctcgttcat gcagtttccg
    10561 catcctcacc gtgcacggtg cctctcctcg agtaaggtca aatcggccag ttccccgccc
    10621 gcaaggggac gatcagcatg tcagcattgt gggaaacgcc ggagcgcaat catggcagcg
    10681 cccggcgggg aggagtaccg gaagcgaatg ctcgtctgat aagcggccta tctggccagc
    10741 ttggctggga tgtggccaaa cccgccgcct ctatgcacat ttacctaaca gctacataac
    10801 aactctcgaa cggcagatcg ggcaaccttc ccccgcgaaa attgatcgag atcagaagaa
    10861 cctgcggtct cacgacacca gtcaatctgc ctccttgtcc tggatgggct tcattcgggt
    10921 tggaggatcg cagcacttgc gaaacatctc cctttccacc gagctcggct gtccttaatc
    10981 tgtatacgat tttgattcgc gtaatcggct cgtctcagcg gcaagcctaa agcaagtgtc
    11041 tgcgcgtatg gatcaagagg agactgcatc aacgatcgat taaccgcaac tgagcattgt
    11101 cagtctcggc agactgccaa gggggagaat tctgatggtg caggggagat gggccatcag
    11161 cgctgttgct gcgctgagcg gggccattgc gtttgcgagt ggggcatacg ctggtagtct
    11221 gaaggactac tctgagccgg aaccaatatc gacaccgaaa tcgacggtga ctttctccgg
    11281 ttcggactgg gcgaaggact catcctactt ctactccggc gttatctacg cgctcaatcg
    11341 ggatctcggg cgcgatgggg ttgtgctgcg cgggttcgcc ggcgtgccga tgttcgaata
    11401 cgatggtggc gccgaaacaa tcgacggcga tggcatccag ggcgatctga tgatcggcta
    11461 catgtggcag cgcccgtttg ccaccttcag tgctttcgtg ggcgtggaat accaggacta
    11521 tgatctcacg cccgatgatc cgaccaacaa ggtgaggggc agcgagacag ggttcaaggt
    11581 ggccgctgat ctcgcctcag cttatgaatc accgctctac ttcaacctga gcggctcata
    11641 ctcgacggcc ttcgacagct actgggcaca gggccgtatc ggctacaacg ccggacggtt
    11701 cgtgatcggc cccgaggtca tcgcggtggg cagcgaaggc ttcgatgctc gtcgggtcgg
    11761 cggattcgcc acgctgcgct tcgagctgtc gccggcgatt tcgagcgaga tcaccgtata
    11821 cgccggtcat cagttcgtgg acgacgaagg cggggaatcg tccaggggcg gcgaaggaac
    11881 ctacggcggc gtcggtttca ctctcgtgtt ctgacgcagg atatcttctg ccataggata
    11941 tccgctggcc agccctgggc gaggccagcg gattttcatt tactcgccga cgtgcacgcg
    12001 ctggggcggc ggcagactca aggtcgt
//
LOCUS       DASVDU010000296        28121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_388115, whole genome shotgun sequence.
ACCESSION   DASVDU010000296 DASVDU010000000
VERSION     DASVDU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28121
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388115"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VF226_19780"
     CDS             <1..628
                     /locus_tag="VF226_19780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7076285.1"
                     /translation="TGAKIGIGEHIKEVQRIFRPVFNATDLKPDGGDFDHLFADGEDF
                     HIGELEVEVIYTPGHTPADISYKIGDAVFVGDTLFMPDYGTARADFPGGDARKLYRSI
                     QKLLALPPETRLFMCHDYKAPGRDTYAWETTVKEQRENNVHLKGGVTEDAFVAMRTAR
                     DATLSAPRLLLPSIQVNIRAGRFPPAEDNGVRYLTIPVKLKGGAEAAV"
     gene            651..1274
                     /locus_tag="VF226_19785"
     CDS             651..1274
                     /locus_tag="VF226_19785"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7076286.1"
                     /translation="MSVEHQSHWERVYSSNAEDQVSWFEAVPETSLSLIRACQLPSDA
                     LILDVGAGDSRLPDALLRQGFANVAVLDVSAGALEKTKARLGPLAKKVRMIAADVVDW
                     RPDFQVDLWHDRATLHFLREPGERAAYAASVRSAVKLGGFVIISGFAPSGPERCSGLA
                     VQRASQSELAALLGPEFELLDAFEKDHTTPRGALQRFLYTRWRRRAD"
     gene            complement(1448..2059)
                     /locus_tag="VF226_19790"
     CDS             complement(1448..2059)
                     /locus_tag="VF226_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076287.1"
                     /translation="MADPIVFAGEVEWSGENPGISLKEDPNGPFVTLASFFRVVLSPH
                     GRGHALVLLQSPGEANPPADRTNYCLYDNEALARYLVSGFVSHFGTFKGLPGLDNLTY
                     RRLDKVETSGDPTSTYTEAVKAGDLDVRLTWSGLGKPFCFVLPPDKSATGKHHMPSLF
                     VGCNDATVTVNGRVLPGKPVPREIAGHTITTAMLAFSETWIRA"
     gene            complement(2199..3743)
                     /locus_tag="VF226_19795"
     CDS             complement(2199..3743)
                     /locus_tag="VF226_19795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX7076288.1"
                     /translation="MTTVLTRKPLFEGAEWNFELLKKAYDAIEEIGVGEMGLNIYTNQ
                     IEVITAEQMLDAYASIGMPNMYRHWSFGKHFAREEVLYRKGARALAYELVINSDPCIN
                     YVMEENTMTMQLLVMAHAAMGHNHFFKNNYLFQQWTDATAILDYLRFAKEYVAECEEK
                     YGLENVEAVLDSAHALMAQGISRHPVRRQSRTDKQERELRRREHEEATYNELWRTVPR
                     SQLDEKPATPEAKEAEAESNALGLPQENLLYFLEKHAPKLDDWKRELLRIVRMLAQYF
                     YPQRQTKVMNEGCATFVHYEIMSRLYDKGLITEGSMLEFMHSHSSVVYQPRFEDKHFN
                     GFNPYALGFAMMRDIKRIAEEPTPEDREWFPEIAGNGDALGTLKEAWANFRDDSFIMQ
                     YLSPKLIRDFRLFHIHDNTGSSFVEVKSIHDEMGYRRVRKVLAEQYDTAARDPDIQVV
                     NADLSGGRRLTLQHRVRRGVLLDKDQCDRTLQHVAHLWGYRVKMLEVDAETGKVLKEH
                     EALPLP"
     gene            complement(3740..5041)
                     /locus_tag="VF226_19800"
     CDS             complement(3740..5041)
                     /locus_tag="VF226_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HEX7076289.1"
                     /translation="MHIVDRRLNPKAKSLGNRQRFLRRAKAEIREAVRDALRRRKVSE
                     VDGGENIRIRSKGLREPSFSLGSNTGERDFVLPGNREYQVGDVIRKPEGGAGGGGQKG
                     SPDGEGEDDFVFSLTREEFLDLFFDDLKLPNLVKAKLKDLRSPEPVRAGLTTEGPPAK
                     LNRLRTMRNSLARRIALRRPRLAELKELEAEIAAEEARDEPDEDRLAQLRARLSRLMH
                     MRKVVAYIDPIDLQYNRHERRPKPKTQAVMFCLMDVSASMTESLKDLAKRFFMLLHLF
                     LSRHYAEVDVVFIRHTTTAQEVDEETFFRDTESGGTVISTALEEMMRVVSERYPPDDW
                     NIYAAQASDGHNFDDDMERCITMLEQDILPICQYFAYIEVMPEAQIAPMQSVAWAGYS
                     EVAEAHPHFAMRRVSSPGEIYPVFRELFSGSASGSEERAAR"
     gene            complement(5060..7006)
                     /locus_tag="VF226_19805"
     CDS             complement(5060..7006)
                     /locus_tag="VF226_19805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX7076290.1"
                     /translation="MTSADEIFSIYAQSYDRQKQHEMPLRQYLEACRDDPMLYAGAAE
                     RMMKAIGEPNIVDTSKDPRLGRVFLNRTIKIYPAFKDFFGLEETIERIVGFFRHAAQG
                     LEERKQILYLLGPVGGGKSSLAERLKELMEQCPVYVLKAGDEISPIFESPLGLFDPVT
                     MGPVLEEKYGIPQRKLTGRMSPWAVKRLDEFGGDISRFNVVKLYPSRLRQICVAKTEP
                     GDENNQDISSLVGKVDIRKLEFHGQNDADAYSYSGGLNRTTQGLLEFVEMFKAPIKML
                     HPLLTATQDGTYVGTENVGALPYQGIIIAHSNESEWQSFRSNKNNEAFLDRICVIQVP
                     YCLRVSEEKKIYEKLLAQSEVSDAPCAPGTLDMLAQFSVLTRLKEHENSSLFSKMRVY
                     DGQSLKDTDPHAKTIQEYRDAAGMNEGMDGTSTRFAYKVLSETFNYDTHEVAADPVHL
                     MFVLEQAVRREQLPEETENRYIEFIKEELAPRYAEFIGNEIQKAYLESYHDFGQNLFD
                     RYVAYADAWIEDQDFKDPDTGQLMNRQLLDQELSKIEKPAGIANPKDFRYEVVKFSLR
                     ARAKNGGKNPNWTSYEKIREVIEKRMFSQVEDLLPVISFGAKKDGETEKKHEEFVNRM
                     VSRGYTHRQVRRLVEWYMRVKKSG"
     gene            complement(7129..7509)
                     /locus_tag="VF226_19810"
     CDS             complement(7129..7509)
                     /locus_tag="VF226_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076291.1"
                     /translation="MIVTDDMIGTATFAAGCRTLALDGVHRRYRSEPIDALAEPKSAS
                     VRFIGCVHFAFKAKLKAVHNEDRPLRCGTIAAATHFRRKLASLSVTSMRAECKNFDIR
                     SGRNRDPGRWVKVRCVSMQGSGEK"
     gene            complement(7661..10675)
                     /locus_tag="VF226_19815"
     CDS             complement(7661..10675)
                     /locus_tag="VF226_19815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7076292.1"
                     /translation="MSGHAQPGWRRAQEKALAAERTASLFSVRWIGQQLGHAHRRNVT
                     VLVCGSAEPGISDARDLGRPPIDFSSYRRIITNHGGRILELSRHGISACWGFPFSGEE
                     DARRAARAALLIIEENSGGAPIACALQTGIAIADGQWSIELASSAFETAQRLLMAGGP
                     ASVVVSGDMRPLVERFFDLQPIQTADCVAWRIAGAEAAYSEAGRVTGALIGHVAERRQ
                     LDEVWLRVVDGQPQCVSISGEAGIGKSRLLRYLELKVKAAGGFWLECSCLPEGRRVPL
                     LPLRQAMQRLLNADEAGLRQFIDGLEHSDRNLISFFLTHRAELLAGEHGMEHLPGERI
                     LTLILDWIAGLAGTGPVGLAFEDMHWADRSTCDFLAKGAGRIAKMGPVCLALTSRDPG
                     VRGLRPTPHLPHTAIRLDKLSVAEIQQLLACNPCGSALPPEKRRQIALRSEGIPLFAM
                     ELARLCTGMRVGEDDLELLLRPGPLNAILSARLDSLRNLKPLAQAAAVIGREFDPGIL
                     ALVLQMEQARLDASLDELVISGILEPTPGADTRRTLRFTHVLLRDAAYASVLEARRRE
                     LHGRIADVLTHDARVAEQEPEIIAGHFSAAGDPKGAFTWWYRAGVRAAELSSTRAAVH
                     HLSQALAARRANPDAGSPREEVDILRLLGIQLAALKGNGAPEAVSTLERCLSLSRKVL
                     GPEGDFDALWALHSCHLVRGEIGRALGMGDKLTACADRSGITERRLRAHRMQGLAKLL
                     GGLIEEAFAHYRIVLDLYDEHRHAPLRLRHASDQGAVALAQLAWGEAIAGRLESSRRH
                     ADAALRLASRLQHPHTSAHVTCVLAARAQTLGEEQAASALAFAGRTLGERHEFPYWIA
                     WADIILGWTGGGGRVTGGAAGTDIERIESAIAAYRRTGALQAVPYALLLVADKALACD
                     RPQRALKAAMEGWQIAWQQGLMLYASELLRVRALAEKKLGSDPAHIANLVDRAVHLAA
                     SQGAETFSSRAAGFRIHPAPQCLDMVLEAPKI"
     gene            complement(10923..11342)
                     /locus_tag="VF226_19820"
     CDS             complement(10923..11342)
                     /locus_tag="VF226_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014764946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7076293.1"
                     /translation="MKVSEVMHPGVHWVEPGTPILEIAKLMRDQDIGSLPVGENDRLV
                     GMVTDRDIVCRGLAEGFDCSTMTARDVMSSGIIYCRDSEELEDAVRIMEQHEVRRLPV
                     INDQKRMVGILSLGDISHAAPRQVSGEVLQSVATPHA"
     gene            complement(11487..12293)
                     /locus_tag="VF226_19825"
     CDS             complement(11487..12293)
                     /locus_tag="VF226_19825"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX7076294.1"
                     /translation="MKHWLGLEQRTALVTGASSGIGRAIAEEFGRCGVAVGVNYHSGR
                     DAAQEVVKSIESEGGKVIALGGDVADPEACRSMVDALVERFGRLDILVANAGVQRDAP
                     FTELSLSDWRRVIDTNLTGAFLCAQEAVRRFRAQPPLPQAAARGNIIFINSVHQVIPW
                     AGHVNYAAAKGGMKLLMESLAQEVAHEKIRVNAIAPGAIKTAINRDAWAEQEAHAALL
                     ELIPYGRVGEPEDVARAAAWLASDLSDYVVGATLFIDGGMTLYPAFREGG"
     gene            complement(12458..14383)
                     /locus_tag="VF226_19830"
     CDS             complement(12458..14383)
                     /locus_tag="VF226_19830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HEX7076295.1"
                     /translation="MSRYHEVYASWKKDPEAFWGEAARDIGWYKLWDRVFDPYMDEYG
                     RWFPGAECNTAYNCLDRHVESGRGEQKALIYDSPVTNTKRSYTYAELTDEVATFAAAL
                     KDLGVGKGDRVVIYMPMVPEAAIAMLACARIGAIHSVVFGGFAASELATRINDAQPVA
                     VVSASCGVEPNRIVPYKPLLDKAIELSEHKPKHCVILQRPMQDAPLVPGRDHDWAALV
                     RDAKARGRKASCVSVAATDPLYILYTSGTTGQPKGVVRDNGGHMVALKWSMKNHYGVE
                     PGEVFWAASDVGWVVGHSYIVYAPLLHGCTTVLYEGKPVGTPDAGAFWRVISEYRVSA
                     MFTAPTAFRAIKKEDPQGEHIKKYDLSAFRTLFLAGERADPETIKWAQERLGVPVIDH
                     WWQTETGWAICGNPVGLGMLPVKPGSPTVVMPGYELCVLDDKGQPLPPGQTGTLAIKL
                     PLPPSCLPTLWNNNERFRKSYLSEFPGYYTTSDAGYQDEDGYFFVMARTDDVINVAGH
                     RLSTGQMEEVVAQHKDVAECAVIGVYDETKGQVPVGFVVINAGVDRDPGEIEAEVIQL
                     VRDTIGPVAAFKNVMVVKRLPKTRSGKILRGTMRQIADGEPWKTPATIDDPAILDEIK
                     EVLAQRGALRRAPAAAV"
     gene            complement(14633..14941)
                     /locus_tag="VF226_19835"
     CDS             complement(14633..14941)
                     /locus_tag="VF226_19835"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX7076296.1"
                     /translation="MAASQLVDKVIYGAQGEQLGTINEVLADQSGRQYIVVGRGGFLG
                     LGEDQFIVPMEQLQMRDGNFYAPQLSKQQLRTMQPYRMGDQNYTIVQRQQQVQVPQAQ
                     "
     gene            14945..16075
                     /locus_tag="VF226_19840"
     CDS             14945..16075
                     /locus_tag="VF226_19840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076297.1"
                     /translation="MPLGHLLLLLLGLPLLGLLLLSLPLLCLSFLLCLLGLLALIGLL
                     LLLRLLGELLLVGVALLLLLGLLSELLLSRLTFRLLRLLTRRDLLELDLRLLLCLLVL
                     DARLTHLLDVHLWLLGALDGRLDTALRRQHLNLRLCGLDLDLRLLPRLGHGNLRLRHL
                     HLNLRLRHLHVHLRLRHSNGNLRLRLLDRYGRRWNANENLRLWLLHRNLRLRLLDPDA
                     GCRRCDLHLWRLLLHDDARLLLPLLPLLLLLSGLLLCLLTLLHLLTLLALLLLLQSRL
                     LHLLKGVLAGPMVPLRRVLACLLQANRSTLGPLLEVLDKLVTWILLLGLELLLYGGAL
                     LALCLRRLPRLHHWLRLLLPLLRRLHLCGCWQNERNRSQQCR"
     gene            16304..17059
                     /locus_tag="VF226_19845"
     CDS             16304..17059
                     /locus_tag="VF226_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7076298.1"
                     /translation="MITDPWFFAVAIPAIVLTGLSKGGFLGGIGGMAVPLMSLVISPV
                     QAAGIMLPILIAMDWIGVWAYRRDWSGPNLKILLPAATGGIIVGWATAAYVTESHVRL
                     LVGVIGVLFTLDHWLKLRPWSVAPGPDLVKGTFWGAVSGFTSFVSHTGGPPFAIYMLP
                     QRLPNAVYAGTAVMMFTAVNLIKVPPYFLLGQFNPENLKTVAVLLPLAPLAMGAGVWL
                     TRRVPQGPFYKILYGCLFVISVKLIWDGLRALV"
     gene            17135..18043
                     /locus_tag="VF226_19850"
     CDS             17135..18043
                     /locus_tag="VF226_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7076299.1"
                     /translation="MESYDVIIVGGGAAGLSAALLLGRARRDVLVCDAGKPRNWASNA
                     LHGYLSRDGMPPAELLETARQELRQYPSVRLIGDTVTGAWPRGDRFEVLLANNGRMQA
                     RKLLLATGIADELPALEGIEAFYGKSVHHCPYCDGWEHRDKAIAVYGSRNKGAKFALM
                     MKQWSQDIVLCTDGRAPPTAETRAQLEKHGIPIRTEKIKRLEGTGAQLERIVFANGDA
                     LPRQALFFTIGQHQRSSLFQHLGVEQGAKGGLNARWPSCQTCVPGVYVAGDASRDVQL
                     LIVAAAEGACAALAINKALLAEDGLG"
     gene            18138..19760
                     /locus_tag="VF226_19855"
     CDS             18138..19760
                     /locus_tag="VF226_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX7076300.1"
                     /translation="MTAYDQHLDRNPANYQPLTPLTLLERAATVHPDHLAVIHGKQRT
                     TYRQLYGRSRQLASALAAQGIGRGDTVSVVLANTPPMLEAHYGVPMTGAVLHAINTRL
                     DAGVIAFQLDHADSKVLIADREFAPTVRAALDRAKVRPLIIEYDDKEFPQPGDPLAGT
                     DYEVFVSTGDPEFAWRMPDDEWDAITLNYTSGTTGNPKGVVYHHRGAYLMCFANVVAA
                     GMGRHPVYLWTLPMFHCNGWCFPWTLSAVAGTHVCLRWVRAKAMYDAIAEHKVTHLCG
                     APIVMSTLLNATPEERQPLPHKVQFVTAAAPPPASVLAAMAEAGFDVTHVYGLTETYG
                     PAVVNDWHDDWDRLPAAERAAQKSRQGVRYPTLEGLKVMDPKTMTEVPADGQTLGEVM
                     FRGNIVMKGYLKNPQATAEAFAGGWFHSGDLGVLYPDGYIQLKDRSKDIIISGGENIS
                     SIEVEDTIYKHPAVAAVAVVAKPDEKWGETPFAFVELKPGAEATAEEIIEWCRKHLAR
                     YKCPRHVAFQTIPKTSTGKIQKFALRELAKSL"
     gene            19776..20588
                     /locus_tag="VF226_19860"
     CDS             19776..20588
                     /locus_tag="VF226_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX7076301.1"
                     /translation="MEDLRLAIAGLGAIGLKVARRVDAGEVEGLTLTAVAARDQAKAE
                     RNISSFRNPPKLVPLDRLADEADVVVECVPAAHFVSVAEPVLQAGRTLMPLSVGALLG
                     HMHLVDLARQTGGRIVVPSGAILGLDAVRAVAEGNVQSVRLITRKPPAGLAGAPLLVE
                     KGISLEGLSEPLMVYSGSAREAAKGFPANLNVSVALSLAGIGPDRTEVELWADPTVTH
                     NTHTILVKSDSSNLTMTIENIPSAENPRTGMVTALSVVAALRRLTAPLVVGT"
     gene            complement(20773..20946)
                     /locus_tag="VF226_19865"
     CDS             complement(20773..20946)
                     /locus_tag="VF226_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX7076302.1"
                     /translation="MSASPHLAELREKHRLLEQKIEEELSRPAVDSIRVNSLKLEKLR
                     LKDEIARLSRRQS"
     gene            21186..21392
                     /locus_tag="VF226_19870"
     CDS             21186..21392
                     /locus_tag="VF226_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX7076303.1"
                     /translation="MQQQDERDIREQLIRLRAEHRELDTQIAALEAQAHADQLQITRL
                     KKKKLQLKDRITALEDRLLPDIIA"
     gene            21503..22006
                     /gene="purE"
                     /locus_tag="VF226_19875"
     CDS             21503..22006
                     /gene="purE"
                     /locus_tag="VF226_19875"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7076304.1"
                     /translation="MSANSQTAPAVGIIMGSQSDWPTMRRAAEALERLGVPFETRIVS
                     AHRTPDRLFAYARSAKDRGLKVIIAGAGGAAHLPGMVASLTPLPVLGVPIESRALKGQ
                     DSLYSIVQMPAGIPVGTLAIGGAGAENAGLLAAQILALNDPTLAARVEEHRARQTAAV
                     AEAPEDA"
     gene            21999..23102
                     /locus_tag="VF226_19880"
     CDS             21999..23102
                     /locus_tag="VF226_19880"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7076305.1"
                     /translation="MPEPARRPEPLPPGSTIGILGGGQLGRMLAMAAAKLGLRTHVYC
                     NEPKCPAFDVSALHTVGDYTDLDRLSAFAATVDAVTYEFENVPVDAAHHLAKVVPVRP
                     GPRALEVAQDRLVEKRFISGLGVPVAPFAQVDDARDLAARLEEFASPAILKTRRLGYD
                     GKGQVSLAPGDDPAAALAAIGHAPAILEQRLSFACEVSVLVVRGCDGRTAFYDIPQNT
                     HSGGILRRSVVPSPLRPAEADLARDMARRIAEALDYVGVLAVEMFYMGQGSDTPLCVN
                     EIAPRVHNSGHWTMDACAIDQFENHIRAVACWPLGTPDRHSDAEMINLIGRDVQSWAE
                     IVAEPGALLHLYGKREPRNGRKMGHVNRLRPKT"
     gene            23271..23516
                     /gene="rpsU"
                     /locus_tag="VF226_19885"
     CDS             23271..23516
                     /gene="rpsU"
                     /locus_tag="VF226_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX7076306.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRNYYEKPSERKA
                     REKAEAVRRARKLARKRAQREGLLPGKGKSKAARGHI"
     gene            complement(23935..24753)
                     /locus_tag="VF226_19890"
     CDS             complement(23935..24753)
                     /locus_tag="VF226_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7076307.1"
                     /translation="MAYSAVKLALILALAYGLILLVANVVQRKLMYDPSAERVAPAEV
                     GLANVEERMIETPDGERVVAWYGRAAPGEPTILYFHGNGGNLALRSERIRNYLNRGRG
                     MLMMSYRGYSGSTGRPSEQANIADARLAYELLIAEGVAPADIIIYGESLGTGVAARIA
                     SEKPIGGLILDSPFTSAVDVGAWHYPYLPVRLLMTDRYEVKSLIGQVRAPVLVVHGEL
                     DRVVPYEMGQAVFEAANEPKKLVSLPFAGHNNHALHGSFDAIQEWIDQIRSRAD"
     gene            complement(24792..25445)
                     /locus_tag="VF226_19895"
     CDS             complement(24792..25445)
                     /locus_tag="VF226_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076308.1"
                     /translation="MNDYGHAHPLNRMETAGRVNGRVGALLIIAAMLLINAVVLAAWW
                     AGHSVVLSKEHSLLEETQLIILIPAFVLFWLGWRYGHDAEKTASGALAMLVAAAFVRE
                     LDVKTLGGPDWFRWLSHHGLQEILLVAMTLPILWYLARRRHHWWGLIRLALAPAAIPL
                     YIAGILLVVAVEFDRDIAVNAELRFWEEFFELNGYLLLVLTGWNHWSIVRNHMAPAA"
     gene            complement(25587..26996)
                     /locus_tag="VF226_19900"
     CDS             complement(25587..26996)
                     /locus_tag="VF226_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX7076309.1"
                     /translation="MSANLVALLYLLSGVLFILALRGLSSPESSRRGNIFGMVGMAIA
                     VLTTLFQISAPDSVLTWLLIIGGIAIGGSIGAYIARSIPMTAMPELVAAFHSLVGLAA
                     VFVAAAALYAPEAFGIADTFGQLRMGSLIEMSLGTAIGAITFTGSVIAFAKLSGRMSG
                     KPILLPARHAINLGLGVLLIVLIAGFAQSGGSAWLFWLIAIAALVFGVLIIIPIGGAD
                     MPVVVSMLNSYSGWAAAGIGFTLGNIALIITGALVGSSGAILSYIMCKGMNRSFISVI
                     LGGFGGEVAGPAAGAEQRPVKQGSAEDAAFMLKNASKVIIVPGYGMAVAQAQHALREM
                     ADALKKAGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINHEFSQADVAYV
                     IGANDVTNPAAKEDPSSPIYGMPVLEVWKAGTVIFNKRSLSSGYAGIDNTLFYRDNTM
                     MLFGDAKKMTEEIVKALEH"
     gene            complement(27009..27386)
                     /locus_tag="VF226_19905"
     CDS             complement(27009..27386)
                     /locus_tag="VF226_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX7076310.1"
                     /translation="MTALLIAILLTPSAAFAAADGGTAVDPFIYQFMVFVIAIFVGYY
                     VVWSVTPALHTPLMSVTNAISSVIVVGALLAVGVALVVSGAALAKLFGFLALVMASVN
                     IFGGFLVTQRMLAMYKKKDAKQR"
     gene            complement(27464..>28121)
                     /locus_tag="VF226_19910"
     CDS             complement(27464..>28121)
                     /locus_tag="VF226_19910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX7076311.1"
                     /translation="AAGTVPAARVFVMGVGVAGLQAIATARRLGAVVTATDVRPATKE
                     QVLSLGAKFVAVEDEEFKQAETQAGYAKPMSAEYQKKQAELIASHIRTQDVVITTALI
                     PGRPAPRLISAAMVESMKPGAVIIDLAAERGGNCELTKPGEIVEVNGVRISGPLNLAG
                     EVAVNASSLYARNLYAFLELMIDKKERAIKMDWDDEIIKGTLVARDGKIVHPNLQQSA
                     "
BASE COUNT         4895 a         8883 c         9010 g         5333 t
ORIGIN      
        1 gacgggcgcg aagatcggca tcggcgagca catcaaggag gtgcaacgca ttttccgccc
       61 ggtcttcaac gcgaccgatc tcaagccgga cggcggcgat ttcgaccatc tgttcgccga
      121 tggagaggac ttccacatcg gtgagctcga agtcgaggta atctacaccc ctgggcatac
      181 tcctgccgac atctcctaca agatcggtga cgcggtgttc gtcggagaca cgctgttcat
      241 gccggactac ggcacggcgc gggcagactt tccgggcggg gatgcgcgca agctctacag
      301 gtcgatccag aagctgcttg cgcttccgcc cgagacgcgg ctgttcatgt gccatgacta
      361 caaggcgccg ggccgcgaca cctacgcctg ggaaacgacc gtcaaggagc agcgcgagaa
      421 caatgttcac ctcaagggtg gggtgaccga ggacgcgttc gtcgcgatgc gcaccgcccg
      481 tgacgcgacg ctgtctgctc cgagactgct gctgccgtcc atccaggtca acatccgcgc
      541 gggccgattc ccgccggcgg aggacaacgg cgtgcgctat ctcactattc cggtgaagtt
      601 gaagggtggc gcggaagcgg cggtctgatc cttaggatgg acggccgggc atgtcggtgg
      661 agcaccagtc gcattgggag cgggtctatt cgagcaacgc cgaggaccag gtgagctggt
      721 tcgaggcggt ccccgagacc tcgctctcgt tgatccgcgc ctgccagctg ccgtccgatg
      781 ccctcattct cgatgtcggc gcgggcgact ctcgcctgcc cgacgccctg ctcaggcaag
      841 gttttgcgaa cgtcgccgtg ctggatgttt cggcaggcgc gctggagaag acgaaggcgc
      901 ggcttgggcc actcgccaaa aaggttcgca tgatcgccgc agacgtcgtc gactggcggc
      961 cggactttca ggtcgacctc tggcacgacc gcgccacact gcatttcctg agggagccgg
     1021 gagagagggc cgcctatgcc gcttcggtgc gctcggcggt caagctcggc ggcttcgtca
     1081 tcatttccgg gttcgcccca tcgggacctg agcgctgcag cggcttggcg gtgcagcggg
     1141 cgagccaatc cgagcttgca gcgctgctcg ggcctgagtt cgagctcctc gacgccttcg
     1201 agaaggatca caccacgccc aggggcgcgc ttcagcgctt cctctacacc cgatggaggc
     1261 ggcgcgcgga ctagagcttt tccgaattca ggtgaaaacg gcggcacaag gcacccgctt
     1321 cacctctccc atggggaaag gtcggcccga agggccgggt ggggggtggc aacagtagcc
     1381 accggcagag tccccttgca actttccccg ctcgccgcga cccttccccc actggagaac
     1441 ctgaagctta cgcgcggatc caggtctctg agaacgccag catagccgtg gtgatggtat
     1501 ggccggcaat ttcgcgtggc accggcttgc ccggcagcac gcggccgttg accgtaacgg
     1561 tcgcatcatt gcagccgacg aagaggcttg gcatgtgatg cttgccggtc gctgatttgt
     1621 ctggcggaag tacaaagcag aacggcttgc cgagcccact ccaagtcagc cgcacgtcga
     1681 gatcccccgc cttgacggcc tcggtgtatg tgctggtcgg gtcgcctgat gtttcgactt
     1741 tgtccagacg acggtaggtc aggttgtcca gtccggggag ccccttgaac gtgccgaaat
     1801 gcgacacgaa cccgctcacc aggtatctcg cgagggcctc gttgtcgtag agacagtagt
     1861 tggtgcgatc ggctggcggg ttggcctcac ccggagactg caacagcaca agggcgtgtc
     1921 cgcggccgtg aggtgagagc acgacgcgga agaagctcgc cagcgtgacg aaggggccgt
     1981 tcggatcctc cttgagagat atgccgggat tctcgccgga ccattcgacc tcgccggcga
     2041 acacgatcgg atctgccatt tgttcctccc aagtctgaac ttcattgtac cgcgcggagg
     2101 ccctatgggc cagccgtcgg cgccaatggc cgaggtcgtg atctgagaga agtcggcggt
     2161 ggtgcgaggg tcagggcgca cggcgtccga ctaccggttc aaggcagagg caatgcctca
     2221 tgctctttca gcactttccc ggtttctgca tccacctcga gcatcttcac ccggtagccc
     2281 cacagatgcg cgacgtgctg aagggtgcgg tcgcactgat ccttgtcgag cagaactccg
     2341 cggcgcacgc ggtgctgcag cgtgaggcgg cgtccgccgg acaggtcggc attcacgacc
     2401 tggatgtcag gatcgcgcgc ggcggtatcg tattgctccg ccagcacctt gcggacccgc
     2461 cgatagccca tctcgtcgtg aatggacttg acctccacga acgacgatcc ggtgttgtcg
     2521 tgaatatgga acagccggaa gtcacgaatg agcttcggcg acaggtactg catgatgaag
     2581 ctgtcgtcgc ggaaattcgc ccacgcttcc ttcagagtcc ccagggcatc gccattgccc
     2641 gcgatttcgg gaaaccactc gcggtcctcg ggcgtcggct cctccgcgat gcgcttgata
     2701 tcgcgcatca tcgcgaagcc cagtgcgtaa ggattgaagc cgttgaaatg cttgtcctcg
     2761 aaccggggct gatagaccac tgacgagtgg ctgtgcatga actcgagcat cgagccttcg
     2821 gtgatgagcc ccttgtcgta gagccggctc atgatctcgt agtgcacgaa ggttgcgcag
     2881 ccctcgttca tcaccttcgt ctgacgctgg gggtagaagt actgcgcgag catgcggacg
     2941 atccgcagca gctcgcgctt ccagtcatcg agcttgggcg cgtgcttttc gaggaaatag
     3001 agcagattct cctgcggcag tcccagagcg ttgctctcag cctcggcttc cttcgcttcg
     3061 ggcgttgccg gcttttcatc gagctgcgag cgcgggacgg ttcgccaaag ctcgttgtac
     3121 gtggcttcct cgtgctcccg ccgcctcaac tcccgttcct gcttgtcggt gcgcgactgc
     3181 cggcggacgg gatggcggct gataccctgc gccatcagcg catgcgcgct gtcgagcaca
     3241 gcctcgacgt tctcgaggcc gtacttctcc tcgcattcgg caacgtactc cttggcaaag
     3301 cgcaggtagt cgaggatcgc ggtggcgtcg gtccactgct ggaacaggta gttgttcttg
     3361 aagaagtgat tatggcccat ggccgcatgc gccatgacga gcagctgcat cgtcatggtg
     3421 ttctcttcca tgacgtagtt gatgcaggga tcggagttga tcaccagctc gtaggcgagc
     3481 gcgcgggcgc ccttgcggta aagcacttct tcccgcgcga aatgcttccc gaacgaccag
     3541 tgccggtaca tgttcggcat gccgatggag gcgtaggcat cgagcatctg ctcggccgtg
     3601 atcacctcga tctggttcgt gtagatattg agccccattt cgccgacgcc gatctcctcg
     3661 atggcgtcgt aggccttctt gagcagctcg aaattccatt ccgccccctc gaaaaggggc
     3721 ttgcgtgtca gcacggtcgt catctcgcgg ctctctcctc gctgccggaa gcggagcccg
     3781 agaacagctc gcggaatacg gggtagattt cacccgggga ggatacgcgc cgcattgcaa
     3841 agtgcggatg agcctccgcg acctcgctgt agcctgccca ggcgacgctc tgcatcgggg
     3901 cgatttgcgc ttcgggcatc acctcgatgt aggcgaagta ctggcagatg ggcaggatgt
     3961 cctgctccag catcgtgatg cagcgctcca tgtcgtcatc gaagttgtgc ccatccgacg
     4021 cctgcgcggc gtagatattc cagtcgtcgg gaggatagcg ctcggagacg acccgcatca
     4081 tctcttcgag agcggtggaa atgacggtgc cgcccgactc ggtatcccgg aaaaaggtct
     4141 cctcgtcgac ctcctgcgcg gtcgtcgtgt ggcggatgaa gaccacgtcc acctcggcgt
     4201 agtgccgcga gaggaacagg tgcagcagca tgaagaaccg cttcgccaag tccttcagcg
     4261 attccgtcat ggaggccgaa acgtccatga ggcagaacat gactgcctgc gtcttgggct
     4321 tcggccggcg ctcgtggcga ttgtactgca ggtcgatggg gtcgatgtaa gcaacgacct
     4381 tgcgcatgtg catcagacgg gaaagccgcg cacgcaactg cgcgaggcga tcctcgtccg
     4441 gctcgtcgcg cgcctcctcg gccgcgatct ccgcctcgag ctccttcagc tccgccagcc
     4501 gcggccgcct gagggcgatg cgtcgggcga ggctattgcg catcgtgcgc aaccggttga
     4561 gctttgccgg cggaccctcg gtggtgaggc cggcgcgcac gggctcgggc gagcgcaggt
     4621 ctttcagctt cgccttgacg aggttcggca gcttgaggtc gtcgaaaaag aggtcgagga
     4681 actcctcccg cgtcaggctg aaaacgaaat catcctcgcc ttccccgtcg gggctgcctt
     4741 tctggccccc gcctccggcg ccgccttccg gtttccggat gacgtctccg acctggtact
     4801 cccggttgcc ggggagcacg aagtcgcgct cgcccgtgtt cgagccgagc gagaagctcg
     4861 gctcacgcag tcccttcgat ctgatgcgga tattttcgcc cccatcgacc tcggagacct
     4921 tgcggcgcct cagggcgtcg cggacggcct cacgaatctc agccttcgcg cgacgaagaa
     4981 agcgctgacg gttcccaaga ctcttggcct tgggattgag acgacggtcg acgatgtgca
     5041 tgccccgctc tcgacctcct caccccgact tcttgacccg catgtaccac tcgacgagcc
     5101 ggcgaacctg ccgatgggta tacccgcggg ataccatgcg gttcacgaat tcctcgtgct
     5161 ttttctcggt ctcgccatct ttcttcgcgc cgaagctgat caccggcagt aggtcctcga
     5221 cctggctgaa catccgcttc tcgatgacct ctcggatttt ctcgtacgat gtccagttcg
     5281 ggttcttccc gccgttcttc gcacgggccc gcagcgagaa tttcacgacc tcgtaacgga
     5341 aatccttcgg gttcgcgatg ccggccggct tctcgatctt tgaaagttcc tggtcgagga
     5401 gctgccggtt catgagctgg ccggtgtcag gatccttgaa gtcctgatcc tctatccagg
     5461 catcggcata ggccacgtac cgatcgaaca ggttttgccc gaaatcgtga taagattcga
     5521 ggtatgcctt ctggatttca ttaccgatga attcggcgta gcgcggcgca agctcttcct
     5581 tgatgaactc gatgtagcgg ttctctgtct cctcgggcag ctgctcacgc cggaccgcct
     5641 gctccagcac gaacatcagg tgcacgggat cggccgccac ttcgtgcgtg tcgtagttga
     5701 aggtttccga cagcaccttg taggcaaagc gggtggaggt gccgtccatg ccttcgttca
     5761 tgccagcggc atcgcgatat tcctggatcg ttttcgcatg cggatcggtg tccttcaggc
     5821 tctggccgtc gtagacccgc atcttcgaga acaggctgga attctcgtgc tccttgagac
     5881 gggtgagcac cgagaactgt gccagcatgt ccagcgtgcc aggggcgcat ggggcatcgg
     5941 acacttcact ctgcgcgagc agcttttcgt aaattttctt ctcttctgaa acgcgcaggc
     6001 aatagggcac ctggatgacg cagatacggt ccaggaacgc ctcgttgttc ttgttcgagc
     6061 ggaagctctg ccactcgctt tcgttggagt gggcgatgat gatgccctgg taggggagcg
     6121 cgccaacgtt ctcggtgccg acgtacgtgc cgtcctgcgt cgccgtcagc agcggatgca
     6181 gcatcttgat gggcgccttg aacatttcga cgaactcgag gagcccttgc gtggtgcggt
     6241 tcaggccgcc cgagtaggaa tacgcgtcgg cgtcgttctg cccgtggaac tcgagtttcc
     6301 gaatatcgac cttgccgacc agcgaggaga tgtcctggtt gttctcgtcc cccggctcgg
     6361 ttttggcgac gcagatctgg cggagccggg acgggtagag cttgacgacg ttgaacctgg
     6421 agatgtcgcc cccgaactcg tccaaccgct tgaccgccca cggcgacatg cgccccgtca
     6481 gcttgcgctg ggggatgcca tacttctcct cgagcaccgg tcccatggtc acggggtcga
     6541 agaggccgag cgggctctcg aaaatcgggc tgatctcgtc acccgccttg agcacgtaga
     6601 ccgggcattg ctccatcagc tccttgagcc gctccgcgag cgaggacttg ccgcctccga
     6661 cggggcccag caggtacaga atctgcttac gttcctccag gccctgggcg gcgtgccgga
     6721 agaagccgac gatccgctcg atggtctcct ccagcccgaa gaagtccttg aaagcgggat
     6781 aaattttgat ggttctgttg aggaacacgc gaccgaggcg cgggtccttc gacgtgtcga
     6841 cgatgtttgg ctcgccgatc gctttcatca tgcgctcagc cgcgccggca tacagcatgg
     6901 gatcgtcgcg gcaggcttcg aggtactgcc gaagcggcat ctcatgctgc ttctggcgat
     6961 cgtagctctg cgcgtagatg ctgaagatct cgtctgcgga ggtcatgctt gttctcgctc
     7021 tcgcgatctc accactgtcg tgacagccac ccttcgagcc atcacccttt catttgaagt
     7081 gggttgcctg ctggcctttg gcaatgtgat tgcccgcttc aaatgcgatc atttctcgcc
     7141 gcttccttgc atactaacac aacgaacttt aacccaacga cctgggtctc gattgcggcc
     7201 gctgcgaata tcgaaatttt tgcattcagc ccgcattgaa gtaacggaca gtgacgccaa
     7261 cttccggcgg aagtgcgtcg ctgcagcgat cgtgccgcac cgcagcggcc gatcttcatt
     7321 atgaacggct ttcagctttg ctttgaacgc gaaatggacg cagccaataa aacgcacgct
     7381 cgcgcttttt ggttcggcca aagcgtcgat cggctccgat cggtatcgtc gatgcacccc
     7441 gtccagtgcc aaggtccgac accctgctgc gaacgtcgca gtcccgatca tatcgtccgt
     7501 tacgatcata gactgattcc ggagggccga atgccgcgca actgcggata aaactaagga
     7561 cgcggggagc ctgtcggagt tcccgccagc gctcgtgtgc gagctatcgc caggcggcgt
     7621 gccatattct tgcgccattt ccggaacttg atgtccggca tcagattttt ggtgcctcca
     7681 gcaccatatc gagacattgt ggcgcggggt gtatccgaaa tcccgcagct cgtgaggaaa
     7741 atgtctcggc tccctggctg gcggcgagat gaaccgcgcg atcgacgaga ttggcgatgt
     7801 gcgccgggtc ggatccgagt ttcttttcag ccaacgcgcg cactctcagc agctctgatg
     7861 cgtagagcat cagtccttgt tgccaggcga tctgccaacc ctccatggcc gccttgaggg
     7921 cccgctgggg gcggtcgcat gcgagggcct tgtcggcaac gagcagcaaa gcgtagggca
     7981 ctgcctggag ggcgcctgtg cgccggtagg ccgcaatcgc gctctcgatt cgctcgatgt
     8041 ccgtccctgc cgccccgccc gtcacgcgtc cgccaccacc ggtccagccg aggatgatgt
     8101 ccgcccaggc gatccagtag ggaaattcgt gccgctcgcc gagcgttctg ccggcgaacg
     8161 ccagagccga ggcagcctgc tcctcgccga gtgtctgcgc ccgggcagcg agtacgcacg
     8221 tcacatgcgc gctggtgtgg ggatgctgta gccgagatgc cagcctcagc gccgcgtcgg
     8281 catgacggcg ggaactctcc agccggccgg cgatcgcctc accccaggcg agctgggcga
     8341 gggcaacggc gccctggtcg gacgcgtgcc gcaggcggag gggggcgtgg cggtgttcgt
     8401 cgtagaggtc cagcacgatc ctgtaatggg cgaaggcttc ttcgatgagg ccgcccagca
     8461 gcttagctag cccctgcatg cgatgggcac gcaggcgccg ctcggtgatg ccgctccggt
     8521 cggcgcaggc cgtcagcttg tcgcccatgc cgagcgcacg accgatctcc ccgcgcacca
     8581 gatggcagga gtgcagcgcc cagagtgcat cgaagtcgcc ctccggcccg aggaccttgc
     8641 gcgacagact taggcatcgt tccagcgtgc tgaccgcctc gggcgctccg ttgcccttga
     8701 gcgctgcgag ctgaatgcca aggagacgca gaatatccac ttcctcccgt ggactgcccg
     8761 catccgggtt ggcccgccgg gccgccagcg cctgactgag gtgatgcacg gccgcccgcg
     8821 tggaggagag ctctgcagcg cgcacgccag ctctgtacca ccacgtgaac gctcctttcg
     8881 ggtcgcctgc agcggagaag tgccccgcta tgatctccgg ctcctgttcc gccacccgcg
     8941 catcatgcgt caggacatcc gcgatacgcc cgtgcagctc acgccgcctt gcctcgagaa
     9001 cggacgcata agcggcatct cgcagcagca cgtgggtgaa gcggagcgtg cgccgggtgt
     9061 cagcaccggg ggtgggctcc agaatgcccg agatgaccag ctcgtcgagg ctcgcatcga
     9121 ggcgcgcttg ttccatctgc agcaccagcg ccagaatgcc gggatcgaac tcccgcccga
     9181 tcacggccgc ggcctgggcc aacggcttca agttccgaag agagtcgagg cgggcagaca
     9241 gaatggcatt gagcggcccc ggccgaagca ggagctcgag atcatcctcg ccgacccgca
     9301 tgccggtgca aagccgcgcc aactccatcg cgaacagggg aatcccctcc gagcgaaggg
     9361 cgatctgcct gcgtttctcg ggaggcagtg ccgagccgca gggattgcag gccagcagct
     9421 gctggatctc tgccaccgac aatttgtcga gccggatcgc tgtgtgcggc agatgcggag
     9481 tgggccggag gccgcgcacg ccgggatcgc gggacgtcaa ggcgaggcag accggcccca
     9541 tcttggcgat ccgtccggcc cccttcgcca gaaaatcgca ggtggatcga tcggcccagt
     9601 gcatgtcctc gaaggccagt ccgacggggc ctgttcctgc caatccggcg atccagtcga
     9661 gtatcagtgt gaggatccgc tcgcctggca agtgctccat gccatgctcg ccagcaagca
     9721 attcggcgcg gtgcgtgaga aagaaagaaa tcaggttgcg atcggagtgc tccagcccgt
     9781 cgatgaactg cctcagcccg gcctcatcgg cgttcaatag gcgctgcatg gcctgtcgta
     9841 gcgggagcag ggggacacgc cggccctcgg gcaggcagct gcactcgagc caaaagccgc
     9901 ccgcggcttt gaccttcaac tcgaggtatc gcaacaggcg cgatttgccg atgccggctt
     9961 cgcctgaaat cgagacgcac tgtggctgac catccacgac gcgcagccac acctcgtcga
    10021 gttgccggcg ctcggcaacg tgcccgatca atgcgccggt gacccggccg gcctcggaat
    10081 aagctgcttc ggccccagcg atgcgccaag cgacgcaatc ggctgtttgg atcggctgaa
    10141 ggtcgaagaa gcgctccacc agagggcgca tgtcaccgct gacgacgaca gacgctggtc
    10201 cgcccgccat caggagtcgc tgtgcggtct cgaaggcgga gctggcaagc tcaatcgacc
    10261 attggccgtc cgcgattgcg atgcccgttt gaagcgcgca tgcaatcggc gcgccgccgg
    10321 agttttcctc gatgatcaga agggcagctc gcgctgcgcg acgcgcgtcc tcctcgccgg
    10381 aaaagggaaa gccccagcag gccgatatac cgtgcctgga cagttcgagg atccgcccgc
    10441 catggttggt gatgatccgg cggtacgatg agaaatcgat tggcggccgc ccgaggtcgc
    10501 gcgcatcaga gatgccgggc tcagccgatc cgcaaaccag caccgtcaca ttacggcggt
    10561 gggcatgccc aagctgctgt ccgatccagc ggaccgagaa taggctggcg gttcgctctg
    10621 cagccagcgc cttttcctgt gctctgcgcc agccgggttg cgcgtggcca gacatgcttc
    10681 aaatcgcacg cgaattgtac tcgtttcggt aataattcat cggaattggt agacccgcgg
    10741 tttggcaagc tcccgcgcgc gccgaggtga acgggagctt aaggtgtcca acaacgcggc
    10801 agaaatgtgc tcgcgtctaa cgttttcgag cggcaggatc gccttgctgg cgcaagaaat
    10861 agagagcgtg ccgccgacct ccggcgacac gctcactgcc gctcaggaaa ctaagacgcg
    10921 ccttatgcgt gcggggtggc cacggactgc aggacctcgc ccgaaacctg acgcggagca
    10981 gcgtgagaga tatcgccgag actcagaatt ccgaccatcc gcttttggtc attgatcacg
    11041 ggcagcctcc ggacttcatg ctgctccatg atacgaacgg catcttccag ctcctcgctg
    11101 tcgcggcagt agatgatgcc gctcgacatc acatcgcgcg cagtcatcgt ggagcagtcg
    11161 aatccctccg caagtccgcg gcagacaata tcgcgatccg tgaccatgcc caccagccgg
    11221 tcgttctcgc cgacgggaag cgagccgatg tcctgatctc gcatcagttt ggcgatttcc
    11281 aaaattggcg tacccggttc cacccagtgc acgcctgggt gcatgacctc actcactttc
    11341 attgttcgct ctcctccaac ggcccgacta ggaatcgatt tgctgggcca agagcttggc
    11401 gccaacactt gctcaacgcc cgagagcgca cggagttcgc cagagctgcc gcgcaaaact
    11461 gccggccgcc gggtccaatc gcgtgatcac ccgccttccc ggaacgcggg atagagcgtc
    11521 atgccgccgt cgatgaagag tgtcgcgccg accacatagt ccgacaggtc ggacgcgagc
    11581 cacgctgcgg cgcgtgccac gtcctcaggc tcgccgacgc ggccgtaggg gatcagctcg
    11641 agcagggcgg catgagcttc ttgctcggcc caggcatcgc ggttgatcgc agtcttgatc
    11701 gcgcctgggg cgatcgcgtt cacgcggatt ttctcgtgcg cgacttcctg tgccaggctc
    11761 tccatcagca gcttcattcc gcccttggct gctgcgtagt tcacgtgccc cgcccacggg
    11821 atcacctgat ggaccgagtt gatgaagatg atgttgccgc gcgcggcagc ctgcggcagc
    11881 ggcggctggg cgcgaaagcg gcggacagcc tcctgcgcgc acaggaatgc tccggtcagg
    11941 ttcgtgtcga tgacccgccg ccaatcgctg agcgaaagct cggtgaacgg tgcgtcgcgc
    12001 tggacgccgg cgtttgcgac aaggatgtcg agacggccaa agcgctcgac gagcgcatcg
    12061 accatggacc ggcaggcctc gggatcggcg acgtcaccgc cgagggcgat gaccttgccg
    12121 ccctcgcttt cgatcgattt cacgacctct tgggcggcat cccgtcccga gtggtagttg
    12181 acgccgacgg caaccccgca cctgccgaac tcttcggcga tcgctctgcc gattccggag
    12241 ctggcgcccg tcaccagggc ggtccgctgc tccaatccca gccagtgctt cacgctcacc
    12301 ctcctcttcg gcgatggagg atgagcacgg tcgcgcccat cctccttgat cccgaggtga
    12361 acgccgccgg cgagggccgg ttcgctggct gcaggcgcga tgagccagca tttggaaccg
    12421 atgttgcccc cagttcccgg cgaggtgatg ctgcgagcta gacggctgcg gcgggcgccc
    12481 gtctcaaggc gccgcgctgc gccagcactt ccttgatctc gtccaggatg gccgggtcgt
    12541 cgatcgtggc cggcgtcttc cagggttcgc cgtcggcgat ctggcgcatc gtcccgcgaa
    12601 ggatcttgcc cgaacgcgtc ttcggcagtc gcttcaccac catgacgttc ttgaaggcgg
    12661 cgaccggccc aatggtgtcg cgcacgagct gaatgacctc cgcttcgatt tcgccgggat
    12721 cgcggtcgac gccggcattg atcacgacga agccgaccgg gacctgcccc ttggtctcgt
    12781 catacacgcc gatcacggcg cactccgcga cgtccttgtg ctgggcgacg acctcctcca
    12841 tctgccccgt ggacaggcgg tgtccggcga cgttgatgac gtcgtccgtg cgcgccatca
    12901 cgaagaagta gccatcctcg tcctgatatc ctgcgtccga ggtggtgtag tagccgggaa
    12961 actccgagag atagctcttt cggaaccgct cgttgttgtt ccacagcgtc ggcaggcacg
    13021 agggcggcag cggcagcttt atggccagtg tgccggtctg ccctggcggc agtggctggc
    13081 ccttgtcgtc gagaacgcag agttcgtagc ccggcatcac gactgtgggg gagccgggct
    13141 tgaccggcag cattccgagc ccgaccggat tgccgcagat cgcccagccc gtctccgtct
    13201 gccaccagtg gtcgatcacc ggcacgccga gccgctcctg tgcccacttg atcgtctcgg
    13261 ggtcggcccg ctctcccgcg agaaacagcg tccggaatgc gctgaggtca tacttcttga
    13321 tgtgctcgcc ctgcgggtct tccttcttga tggcgcggaa ggcggtcggc gcggtgaaca
    13381 tggccgatac gcggtactcg gagatcaccc gccagaatgc acccgcatcc ggagttccga
    13441 ccggcttgcc ctcgtaaagc acggtcgtgc agccatgcag caggggcgcg tagacgatgt
    13501 aggagtgccc cacgacccac ccgacatcgg aggctgccca gaacacctcg cccggctcga
    13561 cgccgtagtg gttcttcatc gaccacttca gcgcaaccat gtggccgcca ttgtcgcgca
    13621 ccacgccctt gggctggcca gtggtgccgg atgtgtagag gatatagagc gggtccgtgg
    13681 cggccacaga gacgcagctc gccttgcgtc cgcgcgcctt ggcgtcgcga acgagggctg
    13741 cccagtcatg atcgcggccc ggcacgaggg gggcgtcctg catcggccgc tgcaggatca
    13801 cgcaatgctt cggcttgtgc tcgctgagct cgatcgcttt gtcgagcagc ggcttgtagg
    13861 ggacgatgcg gttcggctcc acgccgcagg aggccgagac gaccgcgacc ggttgggcat
    13921 cgttgatgcg ggtggcaagc tcggaggcgg caaagccgcc gaaaaccacc gagtggatgg
    13981 caccgatgcg ggcgcaggcg agcatggcga tggccgcctc cggaaccatc ggcatgtaga
    14041 tgacgacgcg gtcgcccttg ccgacgccga ggtctttcag tgcggctgcg aaggtcgcaa
    14101 cctcatccgt gagctcggcg taagtgtagc ttcttttcgt gttcgtgacc gggctgtcgt
    14161 agatgagagc cttctgctcg ccgcggccgc tctcgacgtg gcgatcgagg caattgtatg
    14221 cggtgttgca ctctgcgccc ggaaaccagc ggccatattc gtccatgtag ggatcgaaga
    14281 cccgatccca gagcttgtac cagccgatgt ctcgcgctgc ctctccccag aaggcttcgg
    14341 ggtccttctt ccagctggcg tagacctcat gatatcggct catttcagct tcactccgca
    14401 tgccgctccc gtcccgagaa aatcgcttga gacacatgcc gggacctcag gcgagtttct
    14461 ggatcgtcac actacgtgcg tccgatgcta tgaaacggct gaactgcgcc gagtgcaata
    14521 ggaggttagg ctcatgccga atggcttcct cccggtgccg ggcagcaagg agccggcgaa
    14581 ggtggctccg ccggctctgg cacttgtctc ttggtcagct tcggggtgag gtttactgtg
    14641 cctgggggac ctgcacctgc tgctgcctct gaacgatagt gtagttctgg tctcccatcc
    14701 ggtagggctg catcgtccgg agttgctgct tgctgagctg cggggcatag aaattgccat
    14761 cccgcatctg cagctgctcc atgggtacga tgaattggtc ttcgccgagg ccgaggaatc
    14821 cgccacgccc gacgacgatg tactgccggc ctgattgatc cgcgagcacc tcattgatgg
    14881 ttccgagctg ctcgccctga gcaccgtaga tgactttgtc tacgagctga ctggcagcca
    14941 tcgtttgcct ctggggcatc tgctgctgtt gctgttgggc ctgcctctgc tgggcctgct
    15001 gctgttgagc ctgccgctgc tgtgcctctc gttcttgctg tgcctgctgg gcttgttggc
    15061 tctgatcggc ttgttgctgc tgctgaggct gttgggcgag ctgctgctgg tcggcgtggc
    15121 cctgttgctg ctgctggggc tgctgagtga attgctgctg tccaggctga ctttccgctt
    15181 gctgcgcctg ctgaccagac gggacctgct cgaactggac ctgcggttgc tcctgtgcct
    15241 gctggtactc gatgcgaggc tgacccatct gctcgacgtg cacttgtggc tgctgggcgc
    15301 gctcgatggt cggctggata ccgccctgcg gcggcagcac ctgaacctgc ggctgtgcgg
    15361 gctggacctg gacttgcggc tgctgccgag gctcggtcac ggcaacctgc ggctcaggca
    15421 tctgcacctg aacctgcggt tgcggcatct gcacgtgcac ctgcggctca ggcattcgaa
    15481 cggtaacctc cggctgcggc tgctggaccg ttatggtcgg cgctggaatg cgaacgaaaa
    15541 tctgcggctg tggctgttgc accgtaacct gcggctgagg ctgctggacc cagatgcggg
    15601 gtgccgccgg tgcgacctgc acctctggcg cttgttgctg cacgatgatg cgagactgtt
    15661 gctgcccctc ttgcccttgc tgctcttgtt gtccgggctg ctgctgtgcc tgctgacctt
    15721 gctgcatctg ctgaccttgc tggccctgct gctgctgttg cagagccgcc tgctgcatct
    15781 gctgaagggc gtgctggcag gcccgatggt tcccctgcgt cgagtactgg cgtgcctgct
    15841 ccaggctaac cggagcaccc tgggcccctt gctggaggtg ctggataagc tggtcacatg
    15901 gattttgctg ctgggcctgg aactgctgct gtacggtgga gccctgctgg ccctgtgcct
    15961 gcggcggctg ccgaggctgc accattggct gcggttgctg ttgccgttgt tgcggcgatt
    16021 gcatctgtgc ggatgctggc agaacgagcg caatcgttcc caacagtgcc gctgacacga
    16081 tcattttcat tgctggggcc ttccatttga gaggaggttg acgagatagc cgcctaacct
    16141 gccgccgatg cggttcgttc cgctgcggtt aacgatggcg ggcgctggca ggtgtcatcg
    16201 agtacgggcg gcgttcgtgg cggcgatggc gtccgtaccg gagacggtgt aagaaggtgg
    16261 cccgcgctcg cgcgctcagc cgcttcccac catttctggc cacatgatca ccgacccctg
    16321 gttcttcgcc gtcgcaattc cggcgatcgt tctcactggc ttgtcgaagg gcgggtttct
    16381 cggcggcatt ggcggcatgg ccgtgcccct catgtccctg gtgatctcac cggttcaggc
    16441 cgccggcatc atgttgccga ttctcatcgc catggactgg atcggcgtct gggcctaccg
    16501 gcgcgactgg agcggcccga atctcaagat cctgctgccg gccgcgaccg gcggcatcat
    16561 tgtcggctgg gcgacggcgg cctacgtgac cgagtcccac gtccgcctgt tggtcggagt
    16621 gatcggcgtc ctgttcaccc tggaccactg gctgaagctc aggccatggt ccgtggcgcc
    16681 cggccccgac ctcgtgaagg ggacgttctg gggagcggtg tccggcttca ccagcttcgt
    16741 cagccacacc ggcggaccgc cgttcgcgat ctacatgctg ccgcagcgct taccgaacgc
    16801 ggtctacgct ggaacggcgg tcatgatgtt cacggccgtc aatctcatca aggtgccgcc
    16861 ctatttcctg ctcgggcaat tcaaccctga gaacctgaag accgtggccg tgctgttgcc
    16921 gctcgcgccg ctcgccatgg gagccggcgt gtggctgacg cggcgggttc cccaggggcc
    16981 cttctacaag atcctgtacg gttgcctgtt cgtcatctcg gtcaagctga tctgggatgg
    17041 actccgggct ctcgtctgag actgccgtaa acggtctcgg aaccgcgcct ctgagacggc
    17101 gttgcgctgc aacacgcaat agctttggcg taccatggaa agctacgatg tcatcatcgt
    17161 ggggggcggc gcggctggcc tcagcgcagc cctgctgctt ggccgtgcac gccgcgacgt
    17221 gctcgtctgc gatgcgggca agccgcgcaa ttgggcttcc aacgcgctgc acggctatct
    17281 gagccgcgat ggaatgccgc cggcggagct tcttgagacg gcccggcagg agctgcggca
    17341 gtatccgagc gtgcggctga tcggcgacac agtcactggg gcttggccgc gaggcgaccg
    17401 gttcgaggtc ttgctcgcca acaacggacg catgcaggcc cgcaagcttc tgcttgcgac
    17461 cggcatcgca gacgagctgc cggcgctcga aggcatcgag gccttctatg gcaagtccgt
    17521 gcaccactgc ccctactgtg atggctggga gcaccgcgac aaggcgatcg cggtctatgg
    17581 cagtcgcaac aagggtgcaa agttcgcgct gatgatgaag caatggagcc aggacatcgt
    17641 gctgtgcacg gatggtcggg cgccgccgac tgctgagacg cgggctcagc ttgaaaagca
    17701 cgggatccca attcgcaccg aaaagataaa gcggctggaa ggcaccggcg cgcagctcga
    17761 gcgcatcgtg ttcgccaacg gggatgcgtt gccgcggcag gcgctctttt tcacgatcgg
    17821 ccagcaccag cgctccagct tgttccagca cctcggcgtc gagcaggggg cgaaaggcgg
    17881 cctcaatgca cgttggccgt cctgccaaac gtgtgtcccg ggggtctacg tggcaggtga
    17941 tgcctcgcgg gacgtgcagc tgctgatcgt ggctgcagca gagggggcgt gcgcggcgct
    18001 cgccatcaac aaggcgctgc tggccgagga tgggctcggc tgaaacgccc aagccgcaat
    18061 cggtgaattg cgacgatctc ggtgctgctg tagcgtccgc tgaaaccttc cggagcctgc
    18121 gatccgcatt tcatgccatg accgcttacg accagcacct cgaccgcaat cccgcgaatt
    18181 accagccgtt gaccccgctg acgctgctcg agcgggccgc gaccgtccac ccggatcacc
    18241 ttgcggtgat ccacggtaag cagaggacga cctaccggca actctacggg cgctcgcgtc
    18301 agctcgcctc tgcgctggcc gcgcagggca tcggccgggg cgatacggtt tcggtggtgt
    18361 tggccaacac gccgccgatg ctcgaggcgc actacggtgt gccgatgacg ggtgccgtgc
    18421 tgcacgcaat caacacgcgg ctcgacgccg gcgtgatcgc attccaactc gaccatgccg
    18481 actccaaggt gctgattgcg gaccgggagt ttgcacccac cgttcgggcc gcgctcgacc
    18541 gggcgaaagt ccggccgctg atcatcgagt atgacgacaa ggaatttccc caaccaggcg
    18601 atccgctcgc cggcacggat tacgaggtgt tcgtgtccac aggcgatccc gagttcgcct
    18661 ggcgtatgcc ggacgacgag tgggatgcca tcacgctcaa ctacacctcc gggacaacgg
    18721 gtaacccgaa gggcgtcgtc tatcaccacc gcggcgcgta tctgatgtgc ttcgccaacg
    18781 tcgtcgcggc ggggatggga cggcatccgg tctatctctg gacgctgccc atgttccact
    18841 gcaacggctg gtgctttccc tggactctgt cggccgtagc cggcacgcac gtctgcctgc
    18901 gctgggtccg cgccaaggcg atgtacgacg cgatcgccga gcacaaggtc acgcacctgt
    18961 gcggcgcacc gatcgtcatg tcgacgctgc tcaatgcgac gcctgaggag cggcagccgc
    19021 tgccccacaa ggtgcagttc gtgactgcgg ccgcaccgcc gcctgcgtcc gttctcgcgg
    19081 cgatggcaga ggcaggcttc gatgtcacgc acgtctacgg cctcaccgag acctacgggc
    19141 ccgctgtcgt caatgactgg cacgacgact gggatcgact tcctgcggcc gagcgcgcgg
    19201 cccagaagtc gcgtcagggt gtgcgctacc cgacgctcga agggctcaaa gtgatggacc
    19261 cgaagacgat gaccgaagtc cctgccgatg gccagacgct gggcgaggtc atgttccgcg
    19321 gcaacatcgt catgaagggc tatctcaaga acccgcaggc gacggcggaa gcctttgcgg
    19381 gcggctggtt ccactcgggt gacctcggcg tgctgtaccc tgacggctac attcagctca
    19441 aggatcgctc caaggacatc atcatctcgg gtggcgagaa catctcgtcc atcgaggtcg
    19501 aggacacgat ctacaagcac ccggcggtcg ccgctgttgc agtcgttgcc aagcctgacg
    19561 agaagtgggg tgaaacgccc tttgccttcg tcgagctgaa gcctggcgcg gaggccaccg
    19621 ccgaggaaat tatcgagtgg tgccggaagc atctggcgcg ctacaagtgt ccccggcacg
    19681 tcgcttttca gacgataccc aagacgtcga cgggcaagat ccagaagttc gcattgcgcg
    19741 agcttgcgaa atccttgtga aaattgaggg tagggatgga agacctccgg ctggcaatag
    19801 cgggacttgg cgccatcggg ctgaaggtgg cccgtcgtgt ggatgccggc gaggtcgagg
    19861 ggctcaccct cacggcagtt gcggcgaggg atcaagccaa ggcggagcgc aacatctcca
    19921 gcttccgcaa cccgccgaag ctcgtcccac tcgatcggct tgcggacgag gccgacgtgg
    19981 tcgtggagtg cgtgccagcc gcgcatttcg tatcggtcgc agagccggtg cttcaggcgg
    20041 gccgtacgct gatgccgctg tcggtcggag cgctgctcgg gcacatgcat ctcgtcgatc
    20101 tcgcccggca aacgggcggg cgcatcgtcg tgccgagcgg cgcgatcctc gggcttgatg
    20161 cagtccgtgc ggtcgccgaa ggcaacgtgc agtccgtccg gctcatcacc cggaagccgc
    20221 cggccggtct tgccggtgcg ccgctgctgg tcgagaaagg gatctcgctg gaaggccttt
    20281 ccgagccgct gatggtctat tccggcagcg cacgcgaggc cgcgaagggc ttcccagcca
    20341 acctcaatgt atcggtggcg ctgtctctcg cgggcatcgg gcccgacagg accgaggtcg
    20401 agctatgggc agacccgaca gtgacccaca acacacatac gattctggtc aagtcggact
    20461 cgtccaatct gacgatgacg atcgagaaca tcccatcggc tgaaaatccg cggacgggta
    20521 tggtcaccgc attgagcgtc gtcgcggcgc tgcgccggct caccgccccg ctcgtcgtag
    20581 gcacctgagc tgggcgaaat agcagctttg ccgcaagttt ggaaaaaaac cttcaaggtt
    20641 tgtggcagtg tgccttccac caagtcagtt tggcgcgcaa attacccgtt ccggccgcaa
    20701 cgcctgctcg gccgaaacgt gggccgcgcc gcggcccgat gggtcaggcc gcccctggag
    20761 ccggctcggg ctttagcttt gcctccggct caggcgagcg atttcgtcct tcagcctgag
    20821 tttctccagc ttcaagctgt tgaccctgat ggaatccacc gcgggtctgg acagctcttc
    20881 ctcgatcttc tgctcaagca accgatgctt ctccctcagt tcagcgagat gtggggatgc
    20941 cgacatcttg ccctccgatc gagtttagac ggtataagct tgccagaatt ggtggatgtt
    21001 gtcgaatcaa cgagtgtttc tacaatcaat gcgcaagcac aatcatggac ttagcggttt
    21061 gtcctaacat gaaacattta caaaatgttg tttagatttg tttgcggcac tattgccagc
    21121 atgcgcgttc ccgaagatat tgcccccggg ttcgaagatc aggatccggt gcaccgaatt
    21181 cggccatgca gcagcaggac gaacgtgaca tccgcgagca gttgatcagg ctccgcgccg
    21241 agcaccggga gctcgatacg cagattgctg ccctcgaggc gcaggcgcac gccgaccagc
    21301 tgcagatcac gcggctgaag aaaaagaagc tacaactcaa ggaccggatc acggcgctcg
    21361 aggaccggct cctgccggat attatcgcct gatcgggacg gcgcggttgc aagcggcgcc
    21421 atgacacttg tcaacgggcg gccgggtcag ctatttgccc ctcttttcag gagagcaggg
    21481 cgaagcgcct gatgattcgg acatgagcgc gaacagccag acggcgccgg cggtcggcat
    21541 tatcatgggc agtcagtccg actggccgac catgcggcgc gccgccgaag cgctggagcg
    21601 gcttggagtg ccgttcgaga ccaggatcgt atcggcccat cgcacgccgg acaggctgtt
    21661 tgcctacgca aggtcggcca aggatcgcgg cctcaaggta atcattgccg gagccggcgg
    21721 agcggcgcac ctgccaggca tggtggcttc gctcacgcca ctgccggtgc ttggcgtgcc
    21781 gatcgaaagc cgtgcgctca aggggcagga cagcctctac tccatcgtgc agatgccggc
    21841 aggcattccg gttggaacgc tggcgatcgg cggtgcgggt gccgagaacg ctggccttct
    21901 cgctgcgcag attctcgcgc tcaatgatcc tacactcgcg gcccgcgtcg aggaacatcg
    21961 cgcacgccag accgcggccg tggcagaggc gccggaagat gcctgagccg gcgaggcgtc
    22021 ccgagcccct gccgccgggc agcactatag gtattttggg cggcgggcag ctgggtcgga
    22081 tgctggcgat ggctgcagcc aagctggggc tgcgcacgca tgtttattgc aacgagccca
    22141 agtgtcctgc cttcgacgtc tcggccctgc atacggtcgg cgattacacc gacctcgatc
    22201 ggctgtccgc gttcgctgcg accgtggatg cggtgacgta cgaattcgag aacgtcccgg
    22261 ttgatgccgc ccaccacctc gcgaaagtgg ttcccgtgcg accggggccg cgcgcgctcg
    22321 aggtcgcgca ggataggctg gtggaaaaac ggttcatctc gggcctcggt gtgccggtcg
    22381 ccccgttcgc ccaggtcgat gatgcgcgcg atctcgcggc tcgattggag gaattcgctt
    22441 cgcccgccat cttgaaaaca cgacggctcg gatacgacgg caaggggcag gtcagcctcg
    22501 cgccggggga cgatccggca gcggcgctcg ccgcgatcgg ccacgcaccc gcgatcctgg
    22561 agcagcgcct gtcgttcgcc tgcgaggtct ccgtcctggt ggtccgtggt tgcgacgggc
    22621 gcacagcctt ctatgacata ccgcagaaca cccattccgg cggcattctc cgccgctccg
    22681 tcgtgccgtc accgctccgc ccggcggagg ctgatctggc gcgcgatatg gcccggcgca
    22741 tcgccgaggc gctcgactac gtgggcgtgc tcgccgtcga aatgttctac atgggccagg
    22801 gttccgatac gccgctctgc gtcaacgaga tcgccccgag agtgcacaac agcggccact
    22861 ggaccatgga tgcctgcgcg atcgaccagt tcgagaatca tatccgcgca gtcgcatgct
    22921 ggccgctggg aacgccggat cgccactccg atgccgagat gatcaacctc attggcaggg
    22981 acgttcaatc ctgggcggag attgtcgcag agccgggcgc tctgttgcat ctctacggta
    23041 agcgcgagcc gcgcaacggc cgcaagatgg gtcacgtgaa ccggcttcgc cccaagactt
    23101 gagcatcgct gcaatgtgct gacgtcgaac ctccccatgg acttgacgag gcgtgtttgc
    23161 tatatcgagt gcaacaacta gcgaggtgac caccccttgg tgcggccaag tgggtgtggt
    23221 tgctcgtggt tagaggccag gactattgga cagacgagga taggaaccgc gtgcaggtac
    23281 tcgttcgcga caacaacgtc gatcaagccc tcaaggcgct caagaagaag atgcagcgcg
    23341 agggtatttt ccgggagatg aagctccgga actattacga gaagccctcc gagagaaagg
    23401 cccgcgagaa ggcggaggct gtgcgtcgcg cccgtaagct tgctcgtaag cgcgcgcagc
    23461 gtgagggtct gctgcctggc aagggcaagt cgaaggctgc ccgcgggcac atctgaagtc
    23521 ctttcctttt tcagttttag agctttttcg tttttgggca gaagcacgcc aggccaaggc
    23581 cgcgccccat ctccccgatg ggggagaggg tctgggcgag ggggggcagc cggtggcggc
    23641 tgttgcagcc ccttggccgt ccgatcggga aaacacctcc ccggaggctc tgctgttggc
    23701 gttttcgcct gaactcggaa aagctcttcc ggtgctcctc cctggtgaag ggttgaaaat
    23761 cttcggagcg gggcactcgc gccggggagt tgccctatcg tgcccggctc cggtattcga
    23821 cgagcgggca ccttcgcttc tcctctcgtt tcgcagcgct atcgttccgc cagaatggag
    23881 tcccgatctg aaatcggctt ccggaggggc ggaggcaggc cgctttgttc cgcgtcagtc
    23941 tgcgcgggac cgaatctggt cgatccactc ctgaatggca tcgaacgagc catgcagcgc
    24001 atgattgttg tggccggcga aggggagtga aacgagcttc ttgggctcgt tggcggcctc
    24061 gaacaccgct tgccccattt cgtagggtac gacgcggtcc agctcgccgt gcaccacgag
    24121 caccggcgcg cgcacctgtc cgatcaggga tttcacctcg tagcgatccg tcatcagcaa
    24181 tcgcacgggc aaatacggat agtgccaggc gccgacgtcg acggctgacg tgaacggcga
    24241 atcgaggatc aggccaccga tcggcttttc ggatgcgatc ctggcggcaa cgcccgttcc
    24301 aagcgactct ccgtagatga tgatgtcggc cggggcgacc ccttccgcga tcagcagctc
    24361 gtaggcaagc cgcgcatccg cgatgttcgc ctgctcggat gggcggccgg tcgagccgct
    24421 gtatccgcgg tagctcatca tgagcatgcc tcggccgcga ttaagatagt tgcggatgcg
    24481 ctcggagcgg agcgcgagat taccgccgtt gccgtggaag tagaggatgg tgggctcacc
    24541 cggagcagca cgcccgtacc aggccacgac acgctcgccg tcaggcgttt cgatcatccg
    24601 ctcctccacg ttcgccaggc ccacctcagc cggggcaacg cgctcggcgg aggggtcgta
    24661 catcagcttg cgctgcacga cgttggcgac gagcaggatc agcccgtacg caagcgcaag
    24721 gatcagcgcg agcttaactg ccgaatacgc catcaaggtc ctggatcgtt gtgcccacgc
    24781 gcagtcgagt atcaggccgc cggggccatg tggttgcgga ctatcgacca gtggttccag
    24841 ccggtgagca ccaggagaag atagccgttg agctcgaaga actcctccca gaaccggagc
    24901 tccgcattga cggcgatgtc ccgatcgaat tcaacggcaa cgaccagcag aatgccggcg
    24961 atgtagagcg gtatggcagc cggggcgagc gccaagcgga tcagccccca ccagtgatgc
    25021 ctgcgacgcg cgagatacca gaggatcggc aacgtcatgg cgaccagcag aatctcctgc
    25081 agaccgtgat gcgagagcca cctgaaccag tcgggaccac cgagcgtctt gacgtcgagt
    25141 tcgcggacga acgccgccgc aaccagcatc gcgagggcac cggaggccgt tttctcggcg
    25201 tcatggccat accgccagcc aagccaaaag aggacgaacg ccggtatcag gatgatgagc
    25261 tgggtttcct cgagaaggct gtgctccttg ctgagcacca ccgagtgacc tgcccaccag
    25321 gcggcaagca ctacggcgtt gatcagcagc atggctgcaa tgatgagaag ggcgccgacg
    25381 cggccattga cgcgtccggc ggtttccatc cggttcagcg gatgggcatg gccatagtca
    25441 ttcatcgacc cctcgcccct tcgtgacggc gtggcgaagt gttaaaccat cgcggcctgc
    25501 aaagccatgg caggacagcg atttccggtg agggatcaca caacgcgggg gaaagccggc
    25561 ggtaatcgag ccacagtttg tgttgttcag tgctccagcg ctttgacgat ctcctcggtc
    25621 atcttcttcg cgtcgccgaa cagcatcatc gtgttgtcgc gatagaacag cgtgttgtcg
    25681 atgccggcgt agcctgacga cagggagcgc ttgttgaaga tcaccgtgcc cgccttccag
    25741 acctcgagca ccggcatgcc gtagatcggc gaggacgggt cctccttggc ggccgggttg
    25801 gtgacgtcat tggcgccgat cacgtacgcg acgtcggcct ggctgaactc gtgattgatg
    25861 tcctccagct cgaaaacctc gtcgtagggc acgttcgcct cggccagcag cacgttcatg
    25921 tgtccaggca tacggccggc aacggggtgg atcgcgtact tcacctcgac gcccgccttc
    25981 ttcagggcat cagccatttc gcgaagcgca tgctgggcct gcgcgaccgc catgccgtag
    26041 ccgggcacga tgatgacctt gctcgcgttc ttcagcatga aggccgcgtc ttccgccgag
    26101 ccttgtttga ccggacgctg ctccgctccc gccgccgggc ctgcaacctc gccgccgaac
    26161 ccgccaagga tgacggagat gaacgagcgg ttcatgccct tgcacatgat gtaggacagg
    26221 atcgcgcccg aggagccgac cagtgccccg gtgatgatca gcgcgatgtt gccgagtgtg
    26281 aagccgatgc cggcggcagc ccagcccgaa taggagttca gcatcgacac gacgacgggc
    26341 atgtccgcgc cgccgatcgg gatgatgatc agcacgccga agacgagcgc ggcgatcgca
    26401 atgagccaga acagccacgc cgagccgccg gattgggcga acccggcgat cagcacgatc
    26461 agcagcacgc cgaggccgag attgatggca tgccgcgccg gcagcaagat cggcttgccg
    26521 gacatacggc cggacagctt ggcaaaagca atgacgctgc ccgtgaacgt gatggcgccg
    26581 attgcagtgc cgagtgacat ctcaatcagg ctgcccatgc ggagctgacc gaacgtatcg
    26641 gcaatcccga aggcctcggg cgcatagaga gctgctgcag ccacgaacac cgccgccagg
    26701 ccgaccagtg aatggaaggc agccacgagc tccggcatcg cagtcatcgg gatcgagcgg
    26761 gcgatgtagg caccgatgct gccgccgatg gcgataccgc cgatgatgag cagccaggtc
    26821 agaaccgagt cgggtgccga gatctggaac agggtcgtga ggacggcaat ggccatgccg
    26881 accatgccga agatgttgcc ccggcgtgag ctttccgggc tcgacagtcc gcgcagcgcg
    26941 agaatgaaga ggacgcccga aaggagatag agaagtgcga cgagattggc cgacatgaag
    27001 ggagtcctct agcgctgctt ggcgtccttt ttcttgtaca tggccagcat gcgctgggtg
    27061 acgaggaagc cgccgaagat gttcacggag gccatgacga gagcgaggaa gccgaaaagc
    27121 ttggcgagcg cggctccgga aacgacgaga gccaccccga ccgccagcag cgcgccgacg
    27181 acgatcacgg acgaaatggc gttcgtcacc gacatcagcg gcgtgtgcag ggccggggtg
    27241 acgctccaca cgacgtagta gccgacgaag atcgcgatca cgaacaccat gaactggtag
    27301 atgaacggat cgacagccgt tccgccgtcc gccgccgcga aggctgcaga aggtgttagc
    27361 agtattgcga tcagcaatgc tgtcatgccc tggatccacc cgcctgcacg ccgaacgcgg
    27421 gccaggaggg aatggtcagc cggtctcttg tccaccatgg ctatcatgcg ctctgctgca
    27481 ggttggggtg cacgatcttc ccgtcgcgag cgacgagcgt gcccttgatg atctcgtcgt
    27541 cccaatccat cttgatcgcc cgttccttct tgtcgatcat cagctcgagg aaagcataca
    27601 gattgcgggc ataaaggctc gaggcgttga cggccacctc gccggcgaga ttgagcgggc
    27661 cggaaatccg gacgccattc acctcgacga tctcgcccgg cttcgtcagc tcgcagttgc
    27721 cgccgcgctc ggccgcgagg tcgatgatga ccgcgcccgg cttcatgctc tcgaccatcg
    27781 cggcggagat cagccgcggt gccgggcggc cggggataag cgccgtcgtg atgacgacgt
    27841 cctgcgtgcg gatgtggctg gcgatgagtt ccgcctgctt cttctggtac tcggccgaca
    27901 tgggcttggc gtagccggcc tgggtttcag cctgcttgaa ttcctcgtcc tcgacggcga
    27961 cgaacttcgc gccgagcgaa agcacctgct ccttggtggc cgggcggacg tctgtcgcgg
    28021 tgactacagc gcccaagcgg cgcgcggtcg cgatggcctg cagccccgca acaccgaccc
    28081 ccatgacgaa gacgcgcgcc gcgggcacgg taccggccgc g
//
LOCUS       DASVDU010000297        12838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_453508, whole genome shotgun sequence.
ACCESSION   DASVDU010000297 DASVDU010000000
VERSION     DASVDU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453508"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF226_19915"
     CDS             <1..447
                     /locus_tag="VF226_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX7076312.1"
                     /translation="TAEAMLVPTRIYVKPVLSAIRATGAIKAVAHITGGGLVENLPRV
                     LPAGLAAHVDLSTWRAPAVFGWLARAGQLEPVELLRTFNTGIGLVVVAAKSKADDVIA
                     HLAAQGEAPRIIGELAAGRGEKTQAKGSGTAEAVHFTGQLTFADAI"
     gene            460..1110
                     /gene="purN"
                     /locus_tag="VF226_19920"
     CDS             460..1110
                     /gene="purN"
                     /locus_tag="VF226_19920"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX7076313.1"
                     /translation="MRKKRVGILISGRGSNMRALVEATREPGYPAEIVTVLSNRPKAP
                     GLTWASAEGLPTAVIDHTSYSCREDFESALHEALTSAGVDLVCCAGFMRLMTAGFVEK
                     WRGRMLNIHPSLLPAFKGLDAQAQALAAGVKIAGCTVHFVEPEMDSGPIIAQAAVPVA
                     DDDTPSTLAARILTAEHKLYPHALHLVASGSARLEGGRVINGVSVNQTAQLFSPAL"
     gene            1355..2719
                     /locus_tag="VF226_19925"
     CDS             1355..2719
                     /locus_tag="VF226_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX7076314.1"
                     /translation="MSAPLPSHVQQLGEQSSIWFKLTPLLKGDPDNPMRTLMQMAERY
                     GSVIAVNMANQRVVLLTEPEHFKHVLVTKADNYTKYFDGMKPIFGKSMITVDGQLWQK
                     IRAPQQPAFHPDMFAEYIPYFLEAIRTKMENWRRLAQSGETIEMVEQTWTLAADMICK
                     ALFDRDMPFNPHFVFKCVKTYTDVMNHKAIRLKKVAGEEFEITEEDTAKALEVWGGVP
                     DIVLGADPREHRERTLLTMIEQAVADPNVPEFDRAQAIDEMKQYLWAGTETTALTLAW
                     SLYLTSRYPEAAERIRREGEETYGDRDPTAADYSGLAYTRAVIQETMRIYPPVWGLIR
                     VAAQHDEIGGREIRPGDRVVMFAYGAHHSPKYWDAPEEFHPERWMGDAAKKRVKYTYL
                     PFGAGKRSCIGGAMSQVENTLALSILLRRFRPEYVGREPPRINATVTLTPKGGLPFRI
                     RELS"
     gene            2800..3378
                     /locus_tag="VF226_19930"
     CDS             2800..3378
                     /locus_tag="VF226_19930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076315.1"
                     /translation="MVTSARSTLAISVLALAISASGASGQQNEITTQEAEAYFRQLQQ
                     EATEIVGQQDFDRLDEWLEENIADGAVWQASLTIVADGKRKGFAEMTFDKSDLSRLSP
                     FFASAFQQRISDYSLEVTVNDVVPHGSDTATATVEWTERFSIDVAGDDSETEQDERQF
                     TFEAVATCTHLLQRTEDSFALGLSTCTGEVRL"
     gene            3526..4374
                     /locus_tag="VF226_19935"
     CDS             3526..4374
                     /locus_tag="VF226_19935"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7076316.1"
                     /translation="MPWGHGRAALGQSSTHYGILLSLSVSLLLGACAQAGAPQLTLDQ
                     STDRTPAQSADSRSELDRAIAYWGDQFAKNPRDKTAAISYAMNLKAAGQKKAALSVLQ
                     QSAIYHANDRDFASEYGRLALEFGQIGLAQKLLANAYDPQNPDWRVISAQGTVLAKQG
                     DYKGAIPYYERALQLAPDQPSVINNLAMALAADGQPARAEPLLRRAASHPEADVKVHQ
                     NLALVLSLQGKSAEAEQIRFAMSRPAEFPNGETSLALRSSARDIPARDDVTDGWTTRV
                     AAAQSH"
     gene            complement(4447..5427)
                     /locus_tag="VF226_19940"
     CDS             complement(4447..5427)
                     /locus_tag="VF226_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7076317.1"
                     /translation="MMDLIETVMQPQLMVMTAAAVAAFATALTIIMPILNRDRMSQRM
                     KVMALERDKMRAARIADLNMSRESHSRLRQAPKGFMQELVDRLNLRSVFETDELKAKL
                     KMAGLRGQAPLVTYMFFRVVVPVILTILALFYMFVVLTNLSYPPVVKIAIALGVGIGG
                     FYLPNMMLENMIQRRQQSIKEAFPDALDMLLICVQAGMSIEAAFGKIAKEISSQSIEL
                     AEEFSLTTAELSYLHERRQAYENLGKRTGIAGIKAVATALIQAERYGTPVGQALRVMA
                     KENRDMRMSEAEKKAAALPPKLTVPMIVFFLPVLFVVILGPAIIQVMKLG"
     gene            complement(5493..6491)
                     /locus_tag="VF226_19945"
     CDS             complement(5493..6491)
                     /locus_tag="VF226_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7076318.1"
                     /translation="MFSDELTQLGVLVLVAVAIGAIVFSLLFPYFSGEKQVEKRRHKV
                     VESRAERAARASQAEQASNRRKAVAETLKEIEDRQKARRKLTLRLRLQRAGLDITPRA
                     FWIASAISGAVCAIIVLLAFPGVPMITAAAGAFVGTFGVPRWVLNRLTKRRQNKFLDE
                     FANAIDVIVRGVKSGLPLNDCLGIIARESPQPICGEFRELVEQQRVGVPLSDCFDRMM
                     MRMPLPEVKFFAIVVAIQQQAGGSLSEALGNLSGVLRDRKKLQAKVKALSAEAKASAA
                     VLGSLPFIVMLMVYISTPEYIALLWSTKSGQFMLAFAAVWMTMGLLVMRKMINFKY"
     gene            complement(6494..7945)
                     /locus_tag="VF226_19950"
     CDS             complement(6494..7945)
                     /locus_tag="VF226_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX7076319.1"
                     /translation="MFGKRSSDPAPRKIVTPQPAVAAVAELPAPAATASRRPEPISEP
                     QRRHSEEYYDVKTTVFNALIDTIDLTQLAKLEAASAREEIRDIVSEIIQLKNVVMSIA
                     EQEELLEDICNDVLGYGPLEPLLARDDIADIMVNGAETTYIEVGGKVVRTGVRFADNA
                     QLMNICQRIVSQVGRRVDESSPICDARLPDGSRVNVIVPPLAIDGPALTIRKFKKDRL
                     TLDQLTKLGSISPEGRILLEIIGRVRCNVIVSGGTGSGKTTLLNCLSSCIDHDERIIT
                     CEDSAELQLQQPHVVRLETRPPNLEGEGQITMRDLVRNCLRMRPERIIVGEVRGAEAF
                     DLLQAMNTGHDGSMGTLHANSPREAISRMESMIMMGGFSLPTKTIREMITGAVDIIVQ
                     ASRLRDGSRKITHITEVLGMEGDVVTMQDIMVYEILGEDPQTGKIIGRHRSTGIARPR
                     FWERAEYYGEQHRLAAALAAAEVSDEHGNPLGA"
     gene            complement(7957..9201)
                     /locus_tag="VF226_19955"
     CDS             complement(7957..9201)
                     /locus_tag="VF226_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE family protein"
                     /protein_id="HEX7076320.1"
                     /translation="MDHGELEALRMRARPVPRISIQAFCELPETARALQSAAQDRRLA
                     KTHVHIQMGGVQAAAQYFAANPTPNLILVESTLDGASMLAELDRLAECCDAGTKVIVV
                     GHVNDVLLYRELLKRGVSEYLVAPVVPLQLMESISNLYSDPESDPIGHVVAFVGAKGG
                     VGSSTICHNVAWTMSEVLKTDVVIADLDLAFGTTGLDFNQDPVQGIAEALSSPERLDE
                     VLLDRLLTKCSDHLSIFAAPVVLDRDYDISPDACDIVLDVVRQSVPYVAVDLPHTWTA
                     WTKRILLSADEVVVTAAPDLANLRNAKNLLDLLKQSRHHDGPPHLVLNMVGMPKRPEI
                     SVREFCQNLDLKPAATIEFDAENFGQAANNGQMVEELNKKARAVQPFRELAMTLAHRK
                     ELAQQAPKTALGPLLQKLKIKL"
     gene            complement(9284..10021)
                     /locus_tag="VF226_19960"
     CDS             complement(9284..10021)
                     /locus_tag="VF226_19960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEX7076321.1"
                     /translation="MSKIFDRMPGRSPRRPIVLNALAVSVTALAVGACMHDQGPMVAG
                     WAITDPSQRHPIMVSQEPATIAFKVRRGAYGLTPQQRAQLMRFIDRYQAEDAGNSRLF
                     IHAPSGAANEVDTMQAVAEIRDLLREAGFDEASVMVTAYQDSRSYEPPIRISYMKYTA
                     EAPECGYWGSNLAEDPLNVGTPNLGCAAQANFAAMVANPADLLGPRNASPRASERRDV
                     IWQKYTKGESTVAQKSADERAKVDIGQ"
     gene            complement(10045..11562)
                     /locus_tag="VF226_19965"
     CDS             complement(10045..11562)
                     /locus_tag="VF226_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEX7076322.1"
                     /translation="MAAPLLSPHVIPSASANGYQAIDERAPASHRGHNSVLRIEPDEL
                     GQSRAVQLGRSKSMLVELPRELRDVIVSNPDVMDAVVQSSRRVYLIGKANGQANAFFF
                     DSNGNQILTLEVLIEHDTAALDALLNRLLPGADIKSEILNETVILTGTVRSPVDATRA
                     ADIASRFIVGPTSDANTRHPAKVINMLSVQGEEQVMLRVTVAEVQRSALKQLGINLGA
                     VINSGNFVTSLLTENALPLTAARGLGRLPVVGWTPSETPPLDVWNPVPGNMFGNSGVA
                     GMWSRGNDAVAGAIRALERDGLLKTLAEPNLTAVSGESAKFLAGGKFPIPVPDGNGGY
                     GIQFEEFGVRLTFTPQVLSESRISLKIETTVSELTNEGAIVMESIAVPALKSREAQST
                     VELPSGGSLAIAGLLRDEVRQNIDGFPGLKDLPVLGTLFRSRDFIKEETELVVIVTPY
                     TVRSVSRQQLVRPDDGLAPATDMKANFLGHLNRIYGKTVPLPEGSLKDDYGFIVD"
     gene            complement(11682..12461)
                     /gene="cpaB"
                     /locus_tag="VF226_19970"
     CDS             complement(11682..12461)
                     /gene="cpaB"
                     /locus_tag="VF226_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX7076323.1"
                     /translation="MRRSQVLGVSVALGAGLLAFLGMRGLLSKPAAVVQKNTVELTKV
                     LVARTDIPLGQLVNEGSFRWQDWPKDAVGPSFVSKASKPDAIGHFKGAIARAPLIAGE
                     PITENKLIKPGTGGVLAAILPSGMRAVSTKISEHTAAGKLILPNDHVDVILIRRTRGG
                     EGHTSEILFRNVRVLAIGQQIEAREGKKGSEGNVATLELTPGQAEQLALANAMGEITL
                     SLRSIADIAEASSGSETLGKSKERDAISVLRYGVKSRAYVN"
     gene            complement(12571..>12838)
                     /locus_tag="VF226_19975"
     CDS             complement(12571..>12838)
                     /locus_tag="VF226_19975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEX7076324.1"
                     /translation="DAKLLAAAALWIGLEHLLPFLFWTALLGGALSVILLAYRSILPP
                     LWLLGQSWAMRLHDPKEGIPYGVAIAGAALIVYPSTGWMTGFAG"
BASE COUNT         2489 a         4003 c         3888 g         2458 t
ORIGIN      
        1 acggccgagg cgatgctcgt gccgacgcgg atctatgtga agcccgtgct ctcagccatt
       61 cgcgcgacag gggccatcaa ggccgtcgcg catatcacgg gtggcggtct cgtcgaaaac
      121 ctgccgcgcg tgctgccggc aggccttgcg gcacatgtgg acctgtcgac ttggagggcg
      181 cccgccgtgt tcggatggct cgcgcgtgcc ggacagctgg agccggttga gctgctgcgg
      241 accttcaata ccggcatcgg attggtcgtc gtcgcggcga agtccaaggc agatgacgtg
      301 atcgcgcatc ttgcggccca gggcgaagca ccacggatca tcggagagct cgctgccggc
      361 cgcggggaga agacccaggc caagggcagc ggcacggccg aggcggtgca tttcacggga
      421 caactgacct tcgcagatgc gatctgaacg gacggcacca tgcgcaagaa gcgcgtcggc
      481 attctgatct ccggccgggg ctcaaacatg agggcgctgg tcgaggccac gcgtgagccg
      541 ggctatccgg ccgagatcgt gaccgtcctc tcgaaccggc caaaggcgcc aggcctcacg
      601 tgggcttctg cagagggcct cccaacggca gtcatagacc acaccagtta ttcttgccgc
      661 gaggatttcg agtcggcgct acacgaagcg ttgacctcgg ctggcgtcga tctcgtgtgc
      721 tgcgcagggt tcatgcgcct gatgacggcc ggcttcgtcg agaagtggcg cggccgcatg
      781 ctcaacattc atccgtctct gctgcccgcg ttcaaaggcc ttgatgcgca ggcacaggct
      841 ctggcggccg gcgtcaagat cgccggctgc accgtccact tcgtcgagcc cgagatggac
      901 tcgggtccga tcatcgctca agccgccgtg ccggtggcag atgacgacac gcctagcacg
      961 ctcgcagcca gaatactcac tgctgagcat aagctatatc cgcacgctct ccacctcgtc
     1021 gcctcgggct cggcgcgcct cgaaggcggg cgcgtcatca acggtgttag cgttaaccag
     1081 acggctcagc tcttctcgcc agccctttga tccgcaggta acagcacgct cgcgaacggc
     1141 gcgccaatgt gaagcgggcc gattgcgaaa tgtggaccat gcgaagcgct ggtggtacga
     1201 ggcaggaata tctctggccg gcgttcgcct gcccatgccg tcctcggtcg attgatgtaa
     1261 aagaagacta tggtcgtcag aaccgattcg gtcggatcag catcccaaat agtacccgtg
     1321 ttcggatgac cagactcgcg gagtgaactc gaccatgagc gcaccgttgc cgtcgcatgt
     1381 tcaacaactg ggcgagcaga gctcgatctg gttcaagctg acacctctgc tcaaaggtga
     1441 tcccgacaat cccatgcgca cgctgatgca aatggcggag cgttacggca gcgttattgc
     1501 cgtgaatatg gccaatcagc gtgtcgttct tctgacagag cctgaacact tcaagcatgt
     1561 tctggtgacg aaggccgata attacacgaa atacttcgac ggcatgaagc cgatcttcgg
     1621 caaatcgatg atcaccgtgg atggtcagct ttggcagaag atccgggctc cccagcagcc
     1681 ggcgttccat ccggatatgt tcgccgagta cattccgtat ttcctggagg ccatccgcac
     1741 caagatggag aactggcgcc ggttggccca gtccggcgag acgatcgaga tggtcgagca
     1801 gacttggacg ctggccgcgg acatgatctg caaggcgctg ttcgaccgcg acatgccgtt
     1861 caacccgcac ttcgtcttca agtgcgtgaa gacttacacc gacgtgatga accacaaggc
     1921 catccgcctg aagaaggtgg ccggcgagga gttcgagatc accgaggagg ataccgccaa
     1981 ggccctcgag gtgtggggtg gcgtgcccga tatcgtcctg ggggccgatc cgcgagagca
     2041 tcgcgagcgc acgctcttga ccatgatcga gcaggcggtc gccgacccca acgtgccgga
     2101 gttcgaccgg gctcaggcca tcgacgagat gaagcagtac ctgtgggccg gtaccgagac
     2161 gacggcgctg acgctggcct ggtccctcta tctcacgtcg cggtatccgg aagcggcaga
     2221 gcggatccgt cgcgagggtg aggagacata cggcgaccgc gacccgacgg ccgccgacta
     2281 ttcggggctc gcctacaccc gcgccgtgat ccaggagacg atgcgcattt atcctccggt
     2341 ctggggtctc atccgcgtcg ccgcccagca cgacgagatc ggcgggcgag agatccggcc
     2401 gggcgaccgc gttgtgatgt tcgcctatgg cgcgcaccac agcccgaagt actgggatgc
     2461 accggaggag ttccatcccg agcgctggat gggcgacgcc gcgaagaagc gcgtgaagta
     2521 cacgtatctg ccgttcggcg cgggcaagcg ctcctgcatt ggcggcgcca tgagccaggt
     2581 ggagaacacg ctggccctgt cgatcctgct gcgccgcttc cggcccgagt acgtcggcag
     2641 ggagccgcct cgcatcaacg caaccgtcac gctcacgccg aagggcggcc tgccattccg
     2701 gattcgcgag ctgagctagg ccaggcagtc tctccttgtg aaccgcatcc ggccagagcg
     2761 gttttgcgaa aggggtccag tctgacctgg gagagcatca tggttacctc cgcccgctcg
     2821 acgctcgcca tttcggtgtt ggcgctggcc atttcggcaa gcggagcaag cggccagcaa
     2881 aacgagatca cgacgcaaga ggccgaggcc tacttccggc aactgcagca agaagccacg
     2941 gaaatcgtcg gccagcagga tttcgaccgt ttggatgaat ggctcgagga aaatatcgcc
     3001 gacggtgccg tctggcaggc cagtctcacg atcgtagcag acggcaagcg gaagggcttt
     3061 gccgagatga ctttcgacaa gagcgacctt agtcgcctca gtccgttttt cgcgagcgca
     3121 tttcagcaga ggatcagcga ctattccctg gaagtgaccg tcaacgacgt cgtgccccac
     3181 ggatcagaca cagcgaccgc cacagtcgag tggacggagc gattcagcat cgatgtcgcc
     3241 ggggacgaca gcgagacgga gcaggacgag cggcagttta cgttcgaggc ggtcgcgacc
     3301 tgcacccatc tgcttcagcg caccgaagac agcttcgcac tcggtctgtc cacctgcacc
     3361 ggggaggtcc gcttgtgatt tccggcgggc tttgcctctc cgagccggag cgctctcaat
     3421 tcggcacggc gattaatcct tttttgactc ctgttcatca cattggaccg ttggggaccc
     3481 gagccgcgca atgagcgccg ctcgctggga tcaggggaga tgtcaatgcc gtgggggcac
     3541 gggcgcgccg cgctcggaca gtcgtcgaca cattacggaa ttttgctttc cctctcggtc
     3601 agtcttttgt tgggcgcatg cgcgcaagcg ggcgcaccgc aactcacgct cgaccagagc
     3661 actgatcgca cgccggccca gagtgcggat tcccgctcgg agctcgatcg cgcgatcgca
     3721 tactgggggg atcaatttgc gaaaaatccg cgcgacaaga cggcggccat cagctacgcg
     3781 atgaacttga aggccgccgg ccagaagaag gccgcactga gcgttcttca gcagtccgcg
     3841 atctatcacg ccaacgaccg ggatttcgcc tcggagtacg gacggcttgc cctcgagttc
     3901 ggccagatcg ggctggcgca gaagctgctg gccaatgctt atgacccgca aaatccggat
     3961 tggcgtgtaa tttccgccca gggcaccgtg ttggccaagc agggtgatta caaaggcgcc
     4021 atcccctatt acgagcgcgc cctgcagctc gccccggatc agccgtccgt gatcaacaat
     4081 ctggcgatgg cgttggcagc cgacggacag ccggcgcgag cggaaccctt gctgcggcgc
     4141 gccgcatccc accccgaggc ggatgtgaag gtgcaccaaa acctcgccct ggtcctgagc
     4201 ctccaaggca agtcggccga agctgagcaa atcaggttcg ccatgagccg ccctgcggag
     4261 ttccccaacg gggaaaccag cttggccctg cgttcatcag cacgcgacat ccctgcccga
     4321 gacgacgtca ccgacggctg gacaacgcgc gttgctgctg cccagagcca ctgatgagcg
     4381 cccaatctcg attagaccgt ttcccgccgc ggatggagcc aattggagca atagcgagct
     4441 tagtgatcaa cccagcttca tgacctgaat aatggccgga cccagaatta cgacgaacaa
     4501 aacgggcagg aagaaaacga tcatcgggac ggtcagcttc ggcggcagtg cggccgcttt
     4561 cttttcggct tccgacatcc gcatatcgcg gttctctttt gccatgacgc gcagcgcctg
     4621 cccgaccggc gtaccgtaac gctctgcctg gatgagtgcc gtcgccaccg ccttgatgcc
     4681 agcaatcccc gtgcgttttc cgaggttctc ataggcttgg cgtcgctcgt gcagatagga
     4741 gagctcagcc gtcgtcagac tgaattcttc tgcaagctcg atcgactggc ttgaaatctc
     4801 cttggcgatc ttcccgaatg ccgcttcgat ggacatgccg gcctggacgc agatcagcag
     4861 catgtcgagc gcgtcaggga atgcctcctt tatcgactgc tgcctgcgct ggatcatgtt
     4921 ctccagcatc atgttgggca gataaaagcc gcctattccc acaccgagcg cgatggcgat
     4981 tttcacgacc ggcggatagc tcagattggt gagcaccacg aacatgtaaa acagcgcgag
     5041 gatcgtaagg atgaccggga cgacgacgcg gaagaacata taggtcacga gcggcgcctg
     5101 ccccctgagg cctgccatct tcagcttcgc cttcagctca tcggtttcga aaaccgatcg
     5161 caaattgagc cgatcgacga gctcctgcat gaagcctttc ggcgcctgcc gcagccggct
     5221 gtgactctcc cggctcatgt tgagatcggc aatgcgcgcg gctcgcatct tgtcacgctc
     5281 cagcgccatc accttcatgc gctggctcat ccggtccctg ttgaggatcg gcatgatgat
     5341 cgtgagcgca gtggcgaacg cggcaacagc ggcggccgtc atgaccatga gctgcggttg
     5401 catcaccgtc tcaataagat ccatcatgac agcaaactca ccacccctat aactcgctga
     5461 accttgagcg gccggccccg cgtgaactgc ctttagtatt tgaaattgat catcttgcgc
     5521 atgacgagca ggcccatcgt catccagacc gcggcgaacg ccagcatgaa ctggcccgat
     5581 ttcgtgctcc aaaggagcgc gatgtattcc ggcgtcgaaa tatagaccat caacatgacg
     5641 atgaacggca gtgagcccag caccgcggct gaggctttgg cctctgcgga aagtgccttc
     5701 accttggctt gcaatttttt gcggtcccgc agaacgccgg aaagattgcc gagcgcttcg
     5761 gaaagactgc cgccggcctg ctgctgaatc gcgaccacga tggcgaaaaa cttcacctcg
     5821 gggagcggca tgcgcatcat catgcgatcg aagcagtcgc tcagcggaac gccgacacgc
     5881 tgctgttcca ccagttcgcg aaactccccg cagatcggct gcgggctttc acgcgcgatg
     5941 atccccaagc agtcgttgag cggaagccct gatttgacgc cccgaacgat gacatcgatc
     6001 gcattggcga actcgtcgag aaacttgttt tggcggcgct tcgtgagccg attgaggacc
     6061 cagcgcggca ctccgaacgt gcctacgaaa gcgccagccg cagcggtgat cattgggacg
     6121 cccgggaaag caaggagcac gatgatcgcg cagaccgcgc cggagatcgc actcgctatc
     6181 cagaatgccc gtggcgtgat gtcgagcccc gccctttgga gtcgcagccg cagggtcagt
     6241 tttcgcctcg ccttctgccg atcctcgatt tccttcaaag tctcggcgac ggctttccgg
     6301 cggttcgacg cctgctccgc ctggctcgcg cgcgcggccc gctcggccct gctctcgacg
     6361 actttgtgcc gccgtttttc gacctgcttc tctcccgaga aatacggaaa cagcaggctg
     6421 aatacgatcg cgccgatggc gacggcgacc agcaccagaa cacccagttg cgtgagctcg
     6481 tcactgaaca tgcctacgcc ccgagtggat tgccgtgctc gtcgctaacc tccgcggcgg
     6541 caagcgctgc ggcaagacga tgctgctcgc cgtagtattc agcacgttcc cagaaccgtg
     6601 gacgcgcaat tcccgtcgag cgatgccggc cgatgatctt gcctgtctgg ggatcctctc
     6661 cgaggatttc ataaaccatg atgtcctgca tggtgacgac atcgccttcc atgccaagga
     6721 cctcggtgat gtgtgtaatt ttgcgcgaac cgtcacgcag tcgcgatgcc tgcacgatga
     6781 tatcgactgc gccggtgatc atttcccgaa tggtttttgt cggcagcgag aagccgccca
     6841 tcatgatcat cgattccatg cggctgatgg cctcgcgcgg gctgttcgcg tgcagagtgc
     6901 ccatggagcc gtcgtggccc gtattcatcg cctgcagcag gtcgaaggcc tcggcgccac
     6961 gcacctcgcc cacgatgatc cgctccggac gcatgcgcag gcaattccgg acgagatcgc
     7021 gcatcgtgat ctgtccttcg ccctccaggt tgggcggacg ggtctcgagg cgaaccacgt
     7081 gcggctgctg aagctgaagc tcggccgaat cctcgcacgt gataatgcgc tcgtcgtgat
     7141 cgatgcagct cgaaaggcaa ttgagcaggg tcgtcttccc tgagccggta ccgccggaaa
     7201 cgatgacgtt gcagcggacg cgaccgatga tctccaataa aatcctgccc tctggggaga
     7261 tcgagccgag tttcgtcagt tgatcgagcg tcagtcgatc ttttttgaat ttgcgaattg
     7321 taagggcagg cccatcgatc gccagcggcg gtacaatgac attcacacgc gatccgtccg
     7381 gcaaacgcgc atcgcaaatc gggctcgact cgtcgactcg ccgaccgact tggctgacga
     7441 tccgctgaca aatgttcatc aactgagcgt tgtccgcaaa tctcacgccc gttcggacca
     7501 ccttgccgcc gacctcgatg tatgtcgtct cggctccgtt caccatgatg tcggcgatgt
     7561 cgtcgcgcgc gagaagcggc tctaggggac catagcccag aacgtcgttg cagatgtcct
     7621 cgagcagctc ctcctgctcg gcaatcgaca tgacgacatt cttgagctgg atgatctcgc
     7681 tgacgatgtc gcggatttcc tcgcgcgccg aagccgcctc gagcttcgca agctgcgtca
     7741 ggtcgatcgt gtcgatcagc gcattgaaca ccgtcgtctt gacgtcgtag tactcttccg
     7801 agtgccggcg ttgcggctcg gaaatcggct ctggccggcg cgatgctgtt gcggccggag
     7861 cgggcagctc agcgaccgcg gcaacggcgg gctgtggcgt tacgattttt cgcggagcgg
     7921 gatcactcga acgcttacca aacatctgac gatccctcag agcttgattt tcagtttctg
     7981 gagtagcgga ccgagcgccg ttttcggggc ttgctgcgcc aattccttgc ggtgggcaag
     8041 agtcatggcg agctcgcgga acggctgcac cgctcttgcc ttcttgttca gctcctcaac
     8101 catttgtccg ttgtttgcgg cttgcccgaa gttctcggca tcgaattcga tcgtcgcagc
     8161 cggcttcaga tcgagatttt gacagaactc tcttaccgaa atttccggcc gtttcggcat
     8221 gccgaccatg ttcagcacga ggtgcggcgg cccgtcgtga tgacgtgact gcttcagcag
     8281 gtcgagcagg tttttggcgt tgcgcaagtt ggcgagatcc ggggccgccg tgacgacgac
     8341 ctcatcagca ctcagcagga tgcgtttcgt ccaggccgtc cacgtgtgcg ggaggtcgac
     8401 cgccacatac gggacgcttt ggcgcacgac gtcgagaacg atgtcgcaag cgtcagggga
     8461 aatgtcgtag tcgcggtcca gcacgacggg cgcagcgaaa atgctcaaat ggtccgagca
     8521 tttcgtcagc aggcggtcga gaaggacctc gtcgagacgc tccggactgg agagcgcttc
     8581 ggcaatcccc tgcaccggat cctggttgaa atcgagcccg gtcgtgccga atgcgagatc
     8641 tagatccgca atgaccacat cggttttcag cacttccgac atggtccaag ccacgttgtg
     8701 gcagatcgtg gatgatccaa cgccgccttt ggcaccgacg aacgccacga cgtgcccgat
     8761 tggatccgat tctggatcgc tataaagatt ggagatgctt tccatgagct gcagcggcac
     8821 gaccggcgca acgagatatt cgctgacgcc gcgcttcagc aactcccgat agagcagcac
     8881 gtcgttgacg tgacctacca cgatcacctt cgtgcctgca tcgcagcatt cggcaagcct
     8941 gtcgagctcg gccagcatgc tggcgccatc gagcgtggat tccaccagaa tgagattggg
     9001 tgtcgggttc gcagcgaaat actgagcggc cgcctgcacc ccgcccattt ggatgtggac
     9061 gtgagtcttg gcgagccgcc gatcctgggc cgcgctctgc aaggcgcgcg cggtttcggg
     9121 caattcgcag aaagcctgaa tggaaatgcg cgggacgggt ctcgcgcgca tacgcagagc
     9181 ctcgagctcg ccatgatcca tgacagcttc cgctgcaact gggccaggag ggcccattgg
     9241 ctcgtccaaa agctgcgact gcgcgaactc actcatactg tcctcactga ccaatatcga
     9301 ccttcgctcg ttcgtccgcc gacttctgcg cgaccgtcga ctcgcccttg gtgtacttct
     9361 gccagatcac gtcgcgacgc tcggatgcgc gcggcgaagc gttgcgaggc ccaaggagat
     9421 cggcagggtt cgccaccatg gccgcaaaat tcgcctgcgc agcacacccg agattgggcg
     9481 tccctacgtt cagcggatcc tctgcgagat tgctgcccca gtagccgcat tccggagcct
     9541 ctgccgtgta cttcatgtac gaaatgcgga tcggcggctc gtagctgcgg ctatcctggt
     9601 atgccgtcac catgacggaa gcttcgtcaa agccagcctc gcgcagcaaa tctcggattt
     9661 ccgccaccgc ttgcatcgtg tcgacctcgt tcgccgctcc gctgggcgca tgaatgaaca
     9721 gtctgctatt gccggcatct tcggcctggt agcgatcgat gaacctcatg agctgcgccc
     9781 gctgctgagg cgtgaggccg taagcgccgc gccgcacctt gaacgcaata gtcgccggtt
     9841 cctgagaaac cattatcgga tggcgttgcg acgggtcggt tatcgcccat cccgcaacca
     9901 tggggccctg gtcatgcatg caggcgccaa ccgccagcgc ggtcaccgag acagcgagcg
     9961 cattcagaac aatcggccga cgcggcgagc ggcccggcat cctgtcgaat atcttggaca
    10021 tccttgttcc ccaatgcctg accgctagtc gacgatgaac ccgtagtcgt ccttcaggct
    10081 gccctccggc aacgggacgg ttttgccgta gatccggttc aggtggccga gaaagttggc
    10141 cttcatatcc gttgccggag ccaatccatc atcggggcgg acgagctgct ggcgcgatac
    10201 cgacctcacg gtgtaaggcg tcacgatgac gacgagctcc gtttcctctt tgatgaaatc
    10261 gcggctgcgg aagagggtgc cgagcacggg caaatctttc agccccggaa atccatcgat
    10321 attctggcga acctcatcac ggagcaggcc cgcgatggcg agggagccgc ccgaaggtag
    10381 ctctaccgtt gactgggctt cgcgtgactt caatgcggga actgcgatgc tctccatcac
    10441 gatcgctccc tcgttggtca gctcgcttac cgtcgtttcg attttgaggc tgatgcggct
    10501 ctcggagagc acctgtggcg taaaggtgag acggacgcca aactcctcga actggattcc
    10561 atagccgccg ttgccgtcag gcacgggaat cgggaacttg ccgccggcga ggaacttggc
    10621 gctttcgccc gaaacagccg tcaggttggg ctctgccaat gtcttgagta ggccgtctct
    10681 ttcgagtgcc ctgatggcac cggcgaccgc gtcattgcct ctgctccaca ttccggctac
    10741 cccggaattt ccaaacatat tcccgggaac cggattccaa acatcgagcg gcggcgtctc
    10801 acttggagtc catcccacca ccggcagccg gccaagacca cgtgccgccg ttaggggcag
    10861 tgcgttttca gtgagcaacg aagtaacaaa attcccggag ttgatcaccg cgccgagatt
    10921 gattcccaac tgcttcaaag ccgacctctg cacctcggcc acggtcacgc gcagcatcac
    10981 ctgctcctcg ccctggacgg agagcatgtt gatgaccttg gccgggtgcc gggtgttcgc
    11041 atccgatgtc ggacccacga tgaagcgcga ggctatgtcg gcggcgcggg tcgcatcgac
    11101 ggggctgcga acggtgcctg tcagaatgac ggtctcgttc agaatttccg atttgatatc
    11161 cgcgccgggc agtaagcggt tgagcagagc atcgagtgcg gctgtatcgt gctcgatcag
    11221 aacctcgagc gtcaggatct gattgccgtt gctgtcgaag aagaaggcat tggcctggcc
    11281 gttggccttg ccgatcaggt aaacgcgcct cgagctttgg acgaccgcat ccatgacgtc
    11341 cggattggaa acgatgacgt cgcgcagctc ccgcggcagc tccacaagca tcgatttgct
    11401 gcggccgagc tgcaccgccc ggctttgacc aagttcatcg ggctcgatcc gcagcacgga
    11461 attgtgcccc cgatggctcg cgggagccct ctcgtcaatg gcctggtagc cgttcgccga
    11521 ggccgacgga atgacgtggg gtgataacaa cggagcagcc atcaagaggg ctgcaaccaa
    11581 aaaacgcctg aatttcgtcc gcatggatcg agacctctga ccggagcacc gactcggaga
    11641 cgcacatgac tcacgcgaac tgaacgcgac gaaaactgac ctcaattgac gtaggcgcgg
    11701 gatttcacgc cgtagcgaag cacgctgatc gcatcgcgct ccttggattt gcccagtgtc
    11761 tcggaaccgc tcgacgcctc cgcgatatcc gcgatactgc gcagcgacag agtgatctca
    11821 cccatggcat tggccagcgc gagctgctcg gcttgccccg gtgtgagctc gagtgtcgcg
    11881 acgttgccct cggacccctt tttgccttcc cgcgcctcga tctgctgccc gatcgccagc
    11941 acgcgcacat tgcggaacag gatctcgctg gtgtgtccct cgccgccgcg cgtgcgccgg
    12001 atcaaaatga cgtcgacatg gtcgttggga agaatgagct tgccggccgc ggtatgctcg
    12061 gagatcttcg tcgagacggc gcgcatgccg gacggcagaa tggctgccaa aacgccgcca
    12121 gtgccgggtt tgatcagctt gttctcggtg atcggctcac cggcgatgag cggggcgcgc
    12181 gcgattgcgc ctttgaaatg cccgatcgcg tcgggcttgc tcgctttgga aacgaagctc
    12241 gggccgacgg cgtccttcgg ccagtcctgc cagcggaagc tgccttcatt gacgagttgg
    12301 ccgagaggga tgtcggtgcg tgcgacgagg accttggtca gctcgaccgt gttcttctga
    12361 acgaccgctg ccggcttgct gagaagcccg cgcatgccca ggaacgccag cagtcccgcg
    12421 ccgagcgcga cggaaactcc gagtacctga gaacgtctca tcacacactg cccccaccag
    12481 agcgggtctc cctccgctca acggtgactc tgcaggataa ttctcaatgt gcggttaact
    12541 ttccggcagg cgcccggggg cggcgccgac tcagccagcg aagccagtca tccagccggt
    12601 cgagggataa acgatcagcg cggcgccagc gatcgcgacg ccgtacggga tgccctcctt
    12661 cggatcgtgc agccgcatgg cccaggactg gccaagaagc cagaggggcg ggagaatact
    12721 ccgataagca agtagaatta ctgatagggc accgcccagc aaggctgtcc agaagagaaa
    12781 tggaaggaga tgctccaagc caatccaaag tgccgctgct gccagcaatt tcgcatct
//
LOCUS       DASVDU010000298        10122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_275186, whole genome shotgun sequence.
ACCESSION   DASVDU010000298 DASVDU010000000
VERSION     DASVDU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275186"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VF226_19980"
     CDS             complement(<1..564)
                     /locus_tag="VF226_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7076325.1"
                     /translation="MSQPTTHILHRRIHASIPVAVRGHGIEIFDSSGKSYIDASGGAA
                     VSCLGHGHPAVNEAIKNQLDKLAYTHSSFFTTEVAERLADNLVEHAPAGISHVYFVSG
                     GSEAIEAALKMARQYFVERGEPQRRHFIARRQSYHGNTLGALAVGGNAWRRQQFAPLL
                     FESYHVSPPYAYREQREGETLEAYGQRL"
     gene            complement(565..1455)
                     /locus_tag="VF226_19985"
     CDS             complement(565..1455)
                     /locus_tag="VF226_19985"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEX7076326.1"
                     /translation="MRVRAVEDQLLSAFDGLPTQLQIAARWLLDNGNDVAFLSMREAA
                     RRANVTPATMTRLAQRLGYDGFASLRTRFTDAVRASTRPFRLRAEGLRARRDLDGDAA
                     LITDTLAVVSGHLKALCEPDTLAAMGRAADIIQSSRQVCWVGARSGFAPVYLGSYLMS
                     LIGGPTRLLDQAGGIGLDALGELTPSDALIAVSIAPYTTQTVEAVAYAAERGVQVIAV
                     TDSRAAPISRPASVSIIVPTETPSFLQTMTPAFIVVECLAALVAARRGQAAVDAIARA
                     EVLFDRLGSYASPTLPKRSR"
     gene            1658..2350
                     /locus_tag="VF226_19990"
     CDS             1658..2350
                     /locus_tag="VF226_19990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase"
                     /protein_id="HEX7076327.1"
                     /translation="MDFIFMLTRNDCTVEAPLRVLERVRPLGLRHIGFKDIGASRSEM
                     AALVAGIKASGATSYMEVVSTDAEDCLRSARLARDLEIDCLLGGTQVGKILSILGGSR
                     TRYFPFPGRPLGCPTRLAGTPIEVEAQCRSFMEKGCAGADVLAYRATEADPLDLVAAA
                     RRGLGEKGFLIVAGSVSTPEQVRLLRSAGANAFTIGSAVFDLTFKPGADSIEEQLKAI
                     LSVCSDDAVESA"
     gene            2428..3225
                     /locus_tag="VF226_19995"
     CDS             2428..3225
                     /locus_tag="VF226_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX7076328.1"
                     /translation="MRAVAEFPRHARRNIRAVLTDIDDTLTEEGRLPPIAYEAMDRLK
                     RAGLAVVPVTGRPAGWCDLIARQWPVDGVVGENGAFYFRYDDAQRIMIRRYAKTAEER
                     ERDRRKLEAIREAVLAEVPGAAVAADQSYREADLAIDFCEDVPPLPDESIQRIVSIFH
                     RFGATAKVSSIHVNGWFGTYDKLTTSARFLRDVLDLDVEAAADSVVFVGDSPNDAPMF
                     GFFPHAVGVANVLRFKDVLSAAPRWVTSQPSARGFAELTDLLLEARA"
     gene            3222..4766
                     /locus_tag="VF226_20000"
     CDS             3222..4766
                     /locus_tag="VF226_20000"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEX7076329.1"
                     /translation="MSSDAAAPVQPFDLAIIGGGINGCGIGRDAAGRGLRVVLFEKDD
                     LASGTSSASTKLIHGGLRYLEHYEFRLVREALIEREVLWAIAPHIIRPMRFVLPHHPA
                     MRPAWFLRLGLFIYDHLGGRKRLPGTRTLDLATDPAGAPLKVGFRTRAFEYSDCWVDD
                     ARLVVLNARDAADRGAVIRTRCIVESADRSAEGWTIRVKDRRTGRTEAVAARVLVNAA
                     GPWVDAVDAQLRRGRARHRTRLVRGAHIVVPRLFDHDRAYILQNVDKRIVFAIPYESR
                     FTLIGTTDCDHSGTPDGVEASPEEISYLCDSISAYFKSQVRPGDVVWSFAGVRALADD
                     SAGAAQAATRDYRLELDADGGAPLLSVLGGKITTYRRLAEEALELIGPHLPTVKGREW
                     TRTAPLPGGDFAIDGFRELVSSLRSRYSGLTEPLLDRLARAYGTRASRILGDARRPED
                     LGADFGGGLHEAEVRYLVREEWAESAADIVWRRSKLGLGMSPAEIARLDEWLAQETAA
                     AGTQSG"
     gene            complement(4792..6174)
                     /locus_tag="VF226_20005"
     CDS             complement(4792..6174)
                     /locus_tag="VF226_20005"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX7076330.1"
                     /translation="MTRAGEDGSAWRANLMPPDTGKMPDAPRRRWQTRGRRLAEELSR
                     YPVIGWLGSGVKWLGELGTAPYPPDIRRRLLIVNLIAYLIAVTTAGYALQHVQLDYQT
                     YKPIVFINLALLAMALTVPAMHRFSSIAGGMLIVVSEYVALMAFTMYLGRSSGIHLQY
                     IVAAAAPFVVFGLERLKLVTVVVILGLVLHIVAWFQFPPEKALIPADQELLDSLYTQA
                     AITTVGLIAASIWYAFRLVERARAETDALLRNILPDSVADRLKSRPGELIADTHDEVS
                     VLFADISGFVGLARSLGASKVVHLLNEIVSEFDKLAAKHGVEKIKTIGDAYMAVAGLP
                     DPTHDHAERLARMALDMLAVVERFRAETGLSLQMRIGMAAGPVLAGVIGTRKFSYDVW
                     GDAVNLAARLENLGEPGRIHLCPICRERLVHAFEIESRGLTEIRGVGQQETWFLVGIR
                     RCDSASAGQA"
     gene            complement(6211..6903)
                     /locus_tag="VF226_20010"
     CDS             complement(6211..6903)
                     /locus_tag="VF226_20010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7076331.1"
                     /translation="MSQASVAQLLGSAKLFGKVDEDCRLEIARQMRETSFSAGQSIFA
                     RGDTGKEVYLVLEGRVRLSVLSSEGRELSFTHAERGDVFGEIAALDGGPRTADATAVT
                     AVRAMTLSHTALKRLVETSPAFAEAAISFLCSRIRETDLQLEGVALHRIEVRLARFLL
                     GMLRQRQGNLSGRAKIDLGMSQGELALLLGASRPKVNAALMLLEDMDAIRRNESIIEC
                     NVDELMRMAELD"
     gene            7300..7968
                     /locus_tag="VF226_20015"
     CDS             7300..7968
                     /locus_tag="VF226_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HEX7076332.1"
                     /translation="MAKSRDGSRNRLERPKLVLASASPRRLMLLSQVGITPDALRPAS
                     LDETPHKGEVPRRLAMRLARAKAEAAREQIANDRDLADAFILAADTVVAVGRRTVLKP
                     ETVDEAVAALQLLSGRAHRVLTALCLITPEDRERVKLVDTRVRFKRLSREEIEAYIAS
                     REWRGKAGGYAIQGLAGSFVQKIVGSYTSVVGLPLTETVNLLAGEGYPVHFAWVKSAD
                     TEID"
     gene            7987..8238
                     /gene="yacG"
                     /locus_tag="VF226_20020"
     CDS             7987..8238
                     /gene="yacG"
                     /locus_tag="VF226_20020"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX7076333.1"
                     /translation="MTERNDEESTLRGRCPICGAPTEPEFRPFCSRRCADVDLSRWLR
                     GAYVIPAQSADDEEDELGQAGGGEARPGQGAGQSDRDEL"
     gene            8282..8357
                     /locus_tag="VF226_20025"
     tRNA            8282..8357
                     /locus_tag="VF226_20025"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8315..8317,aa:Phe,seq:gaa)
     gene            complement(8544..9296)
                     /locus_tag="VF226_20030"
     CDS             complement(8544..9296)
                     /locus_tag="VF226_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076334.1"
                     /translation="MASKLEEATMALLGSGVLAIWNGVEEGADQDFIEWHVLEHIPER
                     VGIPGFLRGRRYIAVDGYPRYFNFYETENPDVLTSSPYRDRLNAPTDWTRQNIARFRD
                     TCRTICSVAATQGRGEGAWIETLRFTNVREPEAFRSSVVSSVVPSILVQPGIVGVHLL
                     EGEAAASQGASTEKTLRGQPDEIADWVLLIEAVEEASLRRVREGTASDRLFREAASTA
                     DIVRGFYRLQFALTKSELDRGSVAPKRWVQTG"
     gene            complement(9508..9622)
                     /gene="rrf"
                     /locus_tag="VF226_20035"
     rRNA            complement(9508..9622)
                     /gene="rrf"
                     /locus_tag="VF226_20035"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(9723..>10122)
                     /locus_tag="VF226_20040"
     rRNA            complement(9723..>10122)
                     /locus_tag="VF226_20040"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1884 a         3214 c         3152 g         1872 t
ORIGIN      
        1 gagcctctga ccataggctt ccagagtttc gccctcgcgc tgctcgcggt aggcatacgg
       61 cggggagaca tgatagcttt cgaacagcag cggtgcgaac tgctgacgcc gccaggcatt
      121 gccgccgacg gccagtgcgc cgagcgtgtt gccgtgatag ctctgccggc gtgcgatgaa
      181 gtggcgccgc tgcggctcgc cacgctccac gaaatactgg cgggccattt tcagcgccgc
      241 ctcgatggcc tccgagccgc ccgagacgaa atagacatga gaaatgccgg ccggggcatg
      301 ctcgacgaga ttgtccgcca ggcgctcagc cacttccgtc gtgaagaatg acgagtgcgt
      361 ataggccagc ttgtcgagct ggttcttgat ggcctcgttg accgccgggt gcccatgccc
      421 gaggcacgac actgccgcgc cgccggaggc gtcgatgtac gacttgccgc tgctatcgaa
      481 gatttcgatg ccgtgacctc tgacggcgac agggatactg gcgtggatcc gccggtgcag
      541 aatgtgagtg gttggttggc tcatttacct gcttcgcttg ggtagggttg gactggcata
      601 gctgcccagc cgatcgaaaa gaacctcggc tcgggcgatg gcatcgacag cggcctgtcc
      661 gcgccgcgcg gcgaccagag ctgccaggca ttcgaccacg atgaaggccg gcgtcatggt
      721 ctgcaggaac gacggcgttt cggtcggaac gatgatggac acgctcgcag ggcgtgagat
      781 gggcgctgca cggctgtcgg tgaccgcgat cacctgaacg ccgcgctcgg cggcgtaggc
      841 cacggcttcg acggtctgag tcgtatacgg ggcaatcgag acggctatca gggcatcgga
      901 tggggtcagc tcgcccagag catcgagccc tatgccgccc gcctgatcga gcaggcgggt
      961 gggccctccg atgagcgaca tgagatagga gccgaggtat acgggcgcga agccggagcg
     1021 cgcaccgacc cagcatacct gacggctgct ttggatgatg tcagcggccc ggcccattgc
     1081 ggccagcgta tcgggctcgc acaacgcctt cagatggccg gagaccaccg ccagcgtatc
     1141 cgtgatcagc gcggcgtctc cgtccagatc acgacgcgcg cgtaagcctt cggcgcgcag
     1201 ccgaaagggc ctggtgctgg cgcgtaccgc atcggtgaat cgggtgcgca ggctggcgaa
     1261 accgtcatag ccgaggcgct gggcgagccg tgtcatcgtt gccggcgtga cgttcgcgcg
     1321 gcgagccgct tcgcgcatag ataggaaggc gacgtcgttg ccattgtcga gcagccagcg
     1381 cgcggcaatc tggagctgcg ttggcagccc atcgaacgca gacagcagct ggtcctcgac
     1441 ggcacgtact ctcataacgg gactagatca cgaagttttg ccgtctgcaa cagtcgtttc
     1501 acgagaggca tagaggtact atctgttaca tccgcatacg aactacgtgc gttggcgcca
     1561 agtctcgctc ccaagttgta gctggcctac ggctttgttc aggcatatgt agccatgtca
     1621 gaacggatcg ggcctgagtg gctcgggagc ggagccgatg gacttcattt tcatgctgac
     1681 tcgcaacgac tgcaccgtcg aggcgccgct cagggtgctc gagcgggtga ggccgctcgg
     1741 gttgcgccac atcggcttca aggatatcgg cgcgtcccga tccgagatgg cagccctcgt
     1801 tgcaggcata aaggcttcag gtgccacctc gtacatggag gtcgtctcga cagacgcaga
     1861 ggattgcttg cgctccgcac ggcttgcccg cgatctggag atcgactgcc tcctcggcgg
     1921 aacacaggtc ggtaagatcc tttctattct cggcggtagc cgcacccgtt attttccctt
     1981 cccgggtcga ccactcggct gcccaacccg actcgccggt acgccgatcg aggtcgaggc
     2041 gcagtgccgc tcgttcatgg agaaggggtg cgccggagcc gacgtccttg cgtaccgcgc
     2101 aacggaagcc gacccgctcg atctcgttgc ggccgcccgg cgcgggttag gcgaaaaagg
     2161 ctttctgatc gtcgccggat cggtgagtac gccggagcag gtccgcctgt tgcggagcgc
     2221 tggcgccaat gcattcacga tcggatccgc cgttttcgat ctgacgttca aaccgggtgc
     2281 cgactcaatc gaggagcagc taaaggctat tctctccgtc tgcagcgacg atgcggtcga
     2341 aagcgcttga cctttcgccc ttggaccgcc ttcaagatgc ggaccctacg cagcttcaac
     2401 caactcgacc gagggacccg ccatcagatg cgcgcggtgg cagagtttcc gaggcatgcg
     2461 cgccgcaata tccgcgctgt cctcaccgat atcgatgata cgttgaccga ggaggggcgc
     2521 cttccgccga tcgcctacga ggcgatggac cggctcaagc gtgccggact cgccgtggtg
     2581 ccggtcaccg gccggccggc ggggtggtgc gatctcatcg cgcggcaatg gcccgtcgat
     2641 ggtgtcgtcg gggagaacgg cgcattctac ttccgatacg atgatgcgca gcggatcatg
     2701 atccggcgct acgccaagac cgctgaagag cgggagcggg atcgacggaa gctcgaggcc
     2761 atacgcgagg ctgttctggc tgaagttcct ggagccgcag tggccgccga ccagagctat
     2821 cgcgaggccg atctcgccat cgatttttgc gaggatgtcc cgccgctacc ggacgaaagc
     2881 atccaaagga tcgtctcgat cttccaccgg ttcggcgcaa ccgccaaggt ctcctccatt
     2941 cacgtgaacg gctggttcgg cacctacgac aagctgacca caagcgcccg cttcctgcgg
     3001 gatgttcttg atctcgatgt cgaggccgct gcggacagtg tcgtgttcgt gggcgacagt
     3061 ccgaacgatg caccgatgtt cgggttcttt ccgcacgccg ttggcgtcgc gaatgtgctg
     3121 aggttcaagg acgtcttgag tgcagcaccc cgctgggtga cgtcgcagcc atccgcacgc
     3181 ggattcgccg agctcaccga cctgctcctg gaggcgcggg catgagttcc gatgctgcag
     3241 ccccagtgca gcctttcgat ctggcgatca tcggcggcgg catcaacggc tgcgggatcg
     3301 gccgagacgc ggcggggcgg ggtttgcgcg ttgtcctgtt cgagaaggat gatctggcga
     3361 gcgggacctc gagcgcctcg accaagctga tccatggcgg cctgcgctat ctggagcatt
     3421 acgaattccg cctcgtgcgc gaggcgctga tcgagcgcga ggttctgtgg gcgattgcac
     3481 cccacattat ccggccgatg cgcttcgtgc tgccgcacca tccggcgatg cggcccgcgt
     3541 ggttcctgag gcttggcctg ttcatctacg accacctcgg tggacggaaa cggctgcccg
     3601 gaacgcggac gttggatctc gcaaccgatc ctgcgggcgc gccgctgaag gtgggcttcc
     3661 gtacacgcgc cttcgagtac tccgattgct gggtggacga tgcccggctc gtcgtcctca
     3721 atgcccgcga tgctgccgac cggggcgccg tcatccgaac cagatgcatc gtcgagagcg
     3781 cggaccgatc ggccgaaggc tggaccatca gggtcaaaga ccggcggacc gggcgaacgg
     3841 aagcggtcgc ggcccgcgtg ctcgtcaacg ccgccgggcc ttgggtggat gccgtcgacg
     3901 cacagctgcg ccgaggcaga gcccgccacc gcacccggct cgtccgcggc gcccatatcg
     3961 tcgtaccgag actattcgat cacgaccgcg cctacatcct gcagaatgtg gacaagcgca
     4021 tcgtctttgc cattccctac gaaagccgct tcacgctgat cggcaccacc gattgcgacc
     4081 actcgggcac gccggatggc gttgaagcct cgcccgagga aatctcctac ctctgcgatt
     4141 ccatttccgc ctacttcaag agccaggtgc ggccaggcga tgtggtctgg agcttcgccg
     4201 gcgtgcgtgc tctcgccgat gattccgctg gagcggcgca ggccgctacc cgtgactatc
     4261 gtctggagct cgacgctgat ggcggtgctc cactgctgtc ggtgttgggt gggaagatca
     4321 cgacgtaccg gcgccttgcg gaggaggcac tggagctcat cggtccgcac ctgccgacgg
     4381 taaagggccg cgaatggacc cggaccgcac cgctccccgg tggtgatttc gcaatcgatg
     4441 gatttcggga gctggtctcc tcgctgcgct ctcgatactc tggcctgacc gagccacttc
     4501 tcgatcgcct cgcacgtgcc tacggcaccc gcgcctcacg tatcctcggt gatgcccgca
     4561 gacctgagga tctcggggcc gattttggtg gtggcttgca cgaagccgag gtgcgctatc
     4621 tcgtgcgcga ggaatgggct gaatcggccg ccgatatcgt ctggcgccgc tcgaagttgg
     4681 ggcttggcat gagccccgcc gagatcgccc gcctggatga gtggcttgcg caggaaacgg
     4741 ctgcggcggg aacgcaatcc gggtaatccg ctcgcccgcg ccccgcctcc atcacgcctg
     4801 gcccgcggat gcggaatcac agcgccgaat gccgaccagg aaccacgtct cctgctggcc
     4861 gacgcccctg atttccgtca ggccgcgcga ctcgatctcg aaggcgtgca ccagccgctc
     4921 gcggcagatc gggcacaggt ggatacggcc gggctccccc aggttttcaa gccgcgccgc
     4981 gaggttcacc gcatctcccc acacgtcata gctgaacttg cgggttccga tgaccccggc
     5041 gaggacgggc ccggcagcca tgccgatgcg catctgcagg ctgagccccg tttccgcgcg
     5101 gaagcgctcg acgactgcca gcatgtcgag cgccattcgc gcaaggcgct cggcgtgatc
     5161 atgagtgggg tccggtagac cagcgaccgc catgtaggcg tcgccgatcg tcttgatctt
     5221 ctcgacgccg tgcttggctg ccagcttgtc gaactcgctc acgatctcgt tgagcagatg
     5281 gacgaccttc gacgctccga gactgcgcgc caggccgacg aacccggaga tgtcggcaaa
     5341 gaggacgctc acttcgtcat gggtgtcagc aatgagctca ccgggccgcg acttcagccg
     5401 gtccgccacg ctgtccggca ggatgttgcg caagagcgcg tcggtttccg cccgggcacg
     5461 ctcgaccagc cggaacgcat accagatcga cgctgcgatc aggccgacag tcgtgatcgc
     5521 cgcctgcgtg tagagcgaat cgagcagctc ctgatcggct gggatcagcg ccttctcggg
     5581 cgggaactgg aaccaggcga cgatgtgcag aacgagcccg aggatgacga cgacggtcac
     5641 cagcttcagc cgctccaggc caaacaccac gaagggcgcg gcggcggcaa caatgtactg
     5701 cagatgaata ccggaggacc gaccaagata catcgtgaac gccatgagcg cgacgtattc
     5761 gctgacgacg atgagcatgc cgcccgctat cgagctgaag cggtgcatgg ctggaaccgt
     5821 cagcgccatc gcgagcaggg ccaggttgat gaaaacaatc ggcttgtagg tctggtaatc
     5881 gagctggaca tgctgcagcg catagcccgc cgtcgtgacg gcgatcaggt atgcgatcag
     5941 gttcacgatc agcagtcgcc gccggatgtc ggggggataa ggagcggtgc cgagctcgcc
     6001 cagccacttc acgcctgaac cgagccaacc gatcaccgga tatcgagaca attcctcggc
     6061 gagccttctg cctcgggtct gccatctcct ccgcggcgca tcgggcatct tccccgtatc
     6121 gggcggcatc aggttcgccc gccatgccga tccgtcttca ccggcacgtg tcacgggatg
     6181 tctcctgcgt gcagtcgact actgcggcga tcaatcgagc tcggccatgc gcatcagctc
     6241 atccacattg cactcgataa tgctctcgtt gcgccgaatg gcgtccatgt cctccaacag
     6301 catgagagcc gcattgacct tcggccggct cgctcccagc agcagcgcga gctccccctg
     6361 cgacatgccg agatcaatct tggcccgccc ggagaggttt ccctggcgct ggcgaagcat
     6421 gccgagcaga aaccgcgcca gcctgacctc gatacggtgc agggcgacgc cctcgagttg
     6481 caggtccgtt tcccgaatcc gcgagcaaag gaaggagatg gctgcctcgg caaacgccgg
     6541 cgatgtctca acgagccgct tcagggcggt atgtgaaagt gtcatggccc tgacagccgt
     6601 aacagctgtt gcgtcggccg tacgtggccc gccgtcgagc gcggcgatct cgccgaacac
     6661 gtcgccccgc tcggcgtgcg taaatgagag ctcacggcct tcgctcgaga ggaccgacag
     6721 ccgcacgcgg ccttcgagca ccaggtagac ctccttgccg gtatcgccgc gggcgaagat
     6781 cgactgcccc gcgctgaacg aggtctcgcg catctgcctg gcgatctcca gtcggcaatc
     6841 ctcgtcgact ttgccgaata gtttcgcact cccaagcaac tgggcgacac tcgcctgtga
     6901 catcgtggca gtatccctaa aaagccgaac ggccttccac tgtggtctgc ctgcagagta
     6961 accgacttgg gccgagactg cgagcaatag ccgcaggcta tagggctcat tgctaatgtt
     7021 cgtggcaacg gtgtttcgca aaacacgcgc gaacacttgt cttgacgttg ccgaccgcgc
     7081 attcttgtcg tcgtagctga tgagatccgg cagcccctct aacgaccgtg gaaaccgtca
     7141 tctggattgc cgctgcctga tctgttccat gcttcccaat ccgctccata cggtccctgc
     7201 catcagccaa tgagtcgctg gatgatatca accaggatag gtcacctagc cgggcttggt
     7261 cgcgctgagg ggcatggccg caatacgttg cggcgtgcga tggccaagtc gcgggatgga
     7321 agccggaata ggctcgagcg gccgaaactg gttctggcca gcgcttcacc ccgccggctg
     7381 atgctgctgt cgcaggtcgg catcacgccc gacgccttac gtcccgcctc gctcgacgag
     7441 accccccata agggcgaggt gccaaggcgg ctggcaatgc gcctcgcgcg tgccaaggcc
     7501 gaggctgcac gcgagcagat cgccaatgac cgcgaccttg ccgacgcgtt catcctggca
     7561 gcagatacgg tggtcgccgt gggccgccgc acggtgttga agccagagac ggtcgacgag
     7621 gccgttgctg ccttgcagct cctgtcggga cgcgcccatc gggtgctgac ggcgctctgc
     7681 ctcattaccc ctgaagatcg cgagcgcgtg aagctcgtgg acacgcgtgt gcgcttcaag
     7741 cgcctttcac gagaggagat cgaggcctat atcgcatcaa gagaatggcg cggaaaggct
     7801 ggcggatatg ccatccaggg cctggccggc tcattcgtgc agaagatcgt cggctcctat
     7861 acgagcgtcg tcgggctgcc gctgaccgaa actgtgaatc tgctggcagg cgaagggtac
     7921 cctgtccact tcgcctgggt gaaatccgct gataccgaaa tcgactgacg aaaggcgcat
     7981 cgcctcatga ccgagcgcaa tgacgaggag agcacattgc gcggccgctg ccccatctgc
     8041 ggtgctccca ccgagcccga attccggcca ttttgctccc gcaggtgtgc tgacgtcgat
     8101 ctctcccgct ggctgcgcgg agcctatgtc atcccagcac aatcggcaga tgacgaggaa
     8161 gacgagttgg gccaggccgg tggtggcgag gctcgaccgg gccaaggcgc tggacagtca
     8221 gacagggacg aactataacg cccccacctc gcagccggtc ccgccggcat gcctgagtca
     8281 agcccaggta gctcagttgg tagagcacgt gactgaaaat cacggtgtcg gtggttcgat
     8341 tccgcccctg ggcaccattt tcccgcaaga atgaaaagtt ggccgctatt ttcggctttc
     8401 ggcgcaagcc cacgcgagga cggtctttga tcctcaatat tcggaagtca gcgaggaatt
     8461 tcggcactgg ctcacaccag cactatcgag aagtgccttc gatcatcacg ctgaaggccg
     8521 caccgagaaa caaattggat gggctatcca gtttgcaccc aacgcttcgg cgctactgag
     8581 ccgcgatcga gctccgattt cgtcagcgca aactgcaggc ggtagaagcc gcggactata
     8641 tccgccgtgc tagccgcctc gcgaaataga cggtccgatg ctgtcccttc gcgaactcgg
     8701 cggaggcttg cttcctcgac cgcctcgatc aacagaaccc aatcggcaat ctcgtcgggc
     8761 tgacctcgaa gggtcttttc tgtcgaggca ccctggctcg cggccgcctc gccctccaac
     8821 aaatgcactc cgacgatacc gggttgcacc agtatcgaag gaacgacaga gcttacaacg
     8881 ctcgaacgga atgcttcggg ctcacggaca ttcgtgaacc ggagcgtttc gatccaggcg
     8941 ccctcgcctc ggccttgcgt tgctgctacg gaacaaatgg tgcggcaagt atcgcgaaac
     9001 cgcgcaatat tctgcctcgt ccagtcggtc ggcgcattga gcctgtcacg atagggcgat
     9061 gaagtgagga cgtctgggtt ctcggtttca tagaagttga aatagcgcgg atagccgtca
     9121 acagcgatat atcggcggcc gcgaagaaaa cctgggatcc ctacccgctc cgggatgtgc
     9181 tcaaggacat gccactcgat gaaatcctga tccgcgccct cctcgacgcc gttccagatc
     9241 gccagcaccc cagaacccag caaagccatc gtcgcttcct ccagtttgga tgccatccat
     9301 ttttgcccac ctcagtcggg cagcacaata agaggagcga aagccgagct cagctgctgt
     9361 gaagctccgg atgagcgggg cccaaattct atcggggaga acggagcaat agagatacaa
     9421 aaacaccccg cccaaaattt tgggcggggt gctctgtcat gaatttggaa gaggggatct
     9481 cgattacctt acttcacccc tttgcagacc tggcaacgac ctactctccc acgtcttgag
     9541 acgaagtacc atcggcgctg gggcgtttca cggccgtgtt cggaatggga acgggtgcag
     9601 ccaccccgct attatcacca ggtcggcgaa agggtgaaga aagtgacttc tggatttttc
     9661 tggaattctc accgcccgtc ccgaacgctt tcgcgtccag aatgagcatt gcttaatgag
     9721 agcgattcaa gccattcgag ctattagtac cggtaagctg cgtgcattgc tgcacttcca
     9781 cacccggcct atcaacgtgg tcgtcttcca cggctctcaa gggagtactc gtttcgaggt
     9841 gggtttcccg cttagatgcc ttcagcggtt atcccgtccg cacatagcta ccctgctgtg
     9901 ccgctggcgc gacaacaggt ccaccagagg tgcgtccatc ccggtcctct cgtactaggg
     9961 acagctcctc tcaatactcc gacacccacg gcagataggg accgaactgt ctcacgacgt
    10021 tctgaaccca gctcacgtac ctctttaaat ggcgaacagc catacccttg ggacctgctc
    10081 cagccccagg atgagatgag ccgacatcga ggtgccaaac ga
//
LOCUS       DASVDU010000299        28568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1050997, whole genome shotgun sequence.
ACCESSION   DASVDU010000299 DASVDU010000000
VERSION     DASVDU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 28568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28568
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050997"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VF226_20045"
     CDS             <1..846
                     /locus_tag="VF226_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7076335.1"
                     /translation="DGPKLVDVHAGPLPEQPSLIVLMLQQRTMAQMIERQLTHRAAAR
                     SISGMAATLAHEIKNPLSGIRGAAQLLEPSLSPADRSLAQLICTETDRIRNLVDRMEV
                     FGDERPLAKEPVNIHDVLDHVCQLAGTGFGRGVTIIPRYDPSLPPVPANRDKLIQAFL
                     NLVKNAVEAVEGNGESGRLILTTAFRPGVRLSVPGSGARVSLPLMIEVEDNGPGVPDH
                     LKPYLFDPFVTTKRSGSGLGLALVAKIIGDHGGIIECESEPRRTIFRVLLPMQDRPPQ
                     TSQSD"
     gene            851..2305
                     /gene="ntrC"
                     /locus_tag="VF226_20050"
     CDS             851..2305
                     /gene="ntrC"
                     /locus_tag="VF226_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEX7076336.1"
                     /translation="MARGTILIADDDSAIRTVLNQALARAGYLPRATGNAATLWRWVS
                     QGEGDAVITDVVMPDENAFDLIPRIKKLRPELPIIVMSAQNTLMTALSAAEKGAFEYL
                     PKPFDLNELVSIVGRALAEPSRSAAKSLPENEPDDLPLIGRSPAMQEIYRIIARLTQT
                     DLTVMISGESGTGKELVARVLHDFGRRRSGPFVAINMAAIPRELIESELFGHEKGAFT
                     GAHTRMPGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQEGEYTTVGGRIPLRTDVRI
                     IAATHRELKSQIQQGLFREDLYYRLNVVPLRLPPLRERLGDIDDLVRHFLRQAARSGH
                     GQKVVEAAALERLKRHTWPGNVRELENLVRRLVALYPQDTITAEIVDAELGQALTSPR
                     IDEGRPDENASLSELVENYLGRYFARHGRDLPPPGLYDRVIREVEKPLITAALAATRG
                     NQIRAAELLGLNRNTLRTRIKDLDIQVFKTPGMA"
     gene            2531..4807
                     /locus_tag="VF226_20055"
     CDS             2531..4807
                     /locus_tag="VF226_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX7076337.1"
                     /translation="MTSADPRRAPGDIVDEGRLPFPEANPLAFWIGFVVVILSLVSGF
                     ATYLILTGLTPIVPRDDIVLTVLFINVVLILAMFAVIGWQAIGLAKAWRDKEAGARLH
                     VRIVGMFSIIAALPAILLAIGATTTFSRSLDNWFSTRTRQIIANSLDVANAYLEEHGQ
                     VIRTDIVNMAKDLDDAADTLRDDPEQFRTQVIAQAGLRDLPAAYVIDAEGRPIISAVE
                     DSRLPYVPPPPQVIAHAEAGQVPLLMPSNSYRVAAITKLNAYPGSYLYVLRGVSPKVM
                     GYLRQTQAGVAEYEELRKRRGGLKIAHGLMYFMISMTALLAAIWAGLWFAGRFVAPIR
                     RLIAGAQEVSRGNLNVTLPVRRGEGDLRRLSQTFNTMTAELKMQRDALVSANEQLLER
                     RRFIEAVLTGVSAGVLGLDREGRIRLANRSAEQLLGAGAENLVGRRLEDAVPEIGELL
                     TKAEEKRLAYRLQHQVKLINNNGDERILAVRFTREVEGGEEQGTVVTVDDITELVSAQ
                     RTSAWADVARRIAHEIKNPLTPIILSAERIRRKYGKVITDDRETFDKLTATIERQAGD
                     IKSMVDEFASFARVPKPVMEPGDLRDVVQEPVILFRESHPHIAYELDLPAEPLMGLFD
                     RRLITQAVTNLIKNATEAVESAAEAPEPDWSGRIEAILRSVDGRIEIEVIDNGIGLPK
                     QNRSRLLEPYVTNKGNKGTGLGLAIVQKIVEQHDGMLTLEDAPPGPQRSRGALVRITL
                     PVRNEFTSKTDVKSEAAE"
     gene            4852..6252
                     /locus_tag="VF226_20060"
     CDS             4852..6252
                     /locus_tag="VF226_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7076338.1"
                     /translation="MASDILVVDDEADIRELVSGILEDEGHRCRLARDSDEALRAIEE
                     RRPHLVILDIWLQGSRLDGLEVLSIIKKVHPDLPVVIISGHGNIETAVTAIKRGAYDY
                     IEKPFKADRLILVTMRALEAYNLRREVKHLKERSTQSAEMIGRSPAINQLRHQIDRVA
                     PTNSRVLIRGASGAGKELAARVLHSKSHRAKGPFVVLNAAAMVPDRVEEELFGTEDRT
                     GEARKVGALEEAHTGTLYIDEVADMPLETQGKILRVLVEQKFLRLGGTHKVSVDVRII
                     SSTSRDLEREMAEGRFREDLYHRLNVVPLRVPGLAERREDIPELVNYFVQQIAQSSGL
                     APRRIGEDAIAVLQAHDWPGNVRELRNNIERLMILCGGDPDAVITADMLPEEIGSNVP
                     LPVNGGAEHLMSLPLREAREIFEREYLLAQINRFGGNISRTAEFVGMERSALHRKLRA
                     LGVSSDHRSGVTAGQP"
     gene            6487..7863
                     /gene="trkA"
                     /locus_tag="VF226_20065"
     CDS             6487..7863
                     /gene="trkA"
                     /locus_tag="VF226_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEX7076339.1"
                     /translation="MKVIICGAGVVGLGIAERLAAEGNDVSVIDIDPALVQRANDVLE
                     VRATLGNGAHPDVLERAGARDADMLIAVTLADEVNMIACQVAHSLFDIPTKIARVRAQ
                     SYLAKEYAALFARNCLAIDFIISPEVEVGNMVLRRLQLPGAFEALSFADGRVLAVGIA
                     CEEDCPVVDTPIRQLSELFPDLPAVIVAVVRKGNLFVPRLDEQLKAGDDVYIVAPADQ
                     VARTLKIFGHEEQVARRVIIAGGGNIGLYVAREIEAREHMVRAKIIEANRGRAMQIAE
                     ELNRTVVLHGSSLSEELLREAEAQSADTMVAVTNDDQVNILTSVLAKHLGCKRSLALV
                     NSAGYTPMVRSLGIDAQINPRTITVSRILQFVRRGRIRIVHSIHDGAGELIEAEAIET
                     APIIGKSIREIDMSEGVRFGAIVRNGQVFAPTGSTELEPKDRVILFVRADHVRQVEQL
                     FRVSPDYF"
     gene            8065..8316
                     /gene="hfq"
                     /locus_tag="VF226_20070"
     CDS             8065..8316
                     /gene="hfq"
                     /locus_tag="VF226_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX7076340.1"
                     /translation="MAERAQSLQDTFLNHVRKNKTPLTIFLVNGVKLQGIVTWFDNFC
                     VLLRRDGHSQLVYKHAISTIMPGQPVHLMEQEPAEAQQG"
     gene            8420..9739
                     /gene="hflX"
                     /locus_tag="VF226_20075"
     CDS             8420..9739
                     /gene="hflX"
                     /locus_tag="VF226_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7076341.1"
                     /translation="MLQTTGSPGTAKRSRAGNGTRGATLHSPQDRLAEAAGLAEAIGL
                     EVRAKLIVPVARPQPATLFGSGKVEELKQLVAEHEAGLVVIDHAVSPIQQRNLERAWN
                     AKVLDRTGLILEIFGRRARTREGRLQVELAHLTYQKSRLVRSWTHLERQRGGYGFLGG
                     PGETQIETDRRLIQERIDAIRKELDVVVRTRELHRSGRRRVPYPLVAIVGYTNAGKST
                     LFNKLTGAGVVAMDQVFATLDPTMRELRLPSGRRVILSDTVGFISDLPTSLVAAFRAT
                     LEEVIEADLILHVRDISHPETQAQRADVERVLRELGIEVDRPDGLILEVWNKIDLLPP
                     ESLEELQHAAERAQQKPLLVSAVEERGLDALLEAIDAQLGRADEVLTIAVPATAGNLL
                     AWLHENAQVLARETAETGQTICQVRIARAKKGRLFSRLRQNGLAVAS"
     gene            complement(9997..10932)
                     /locus_tag="VF226_20080"
     CDS             complement(9997..10932)
                     /locus_tag="VF226_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7076342.1"
                     /translation="MGAVNRLILDKAVSDVELIPQLAQHLIDSGGKRMRPMLTIAAAK
                     LCGYTGDGHVPLAAAVEFMHTATLLHDDVVDESDFRRGKKAARLVWGNQASVLVGDFL
                     LGQAFRMMVEVGSLQALKILSNAAAVIAEGEVMQLAAAKNTATTEDEYLAIINAKTAA
                     LFSAATEVGGAIANRPADEQAALKSYGKTLGIAFQLVDDALDYSGDSARLGKSVGDDF
                     REGKITLPVILAFRRGSDEERAFWRRTLGEGDIREGDFEHAMSLMRRHKAIEATLERA
                     RYYGSMAEDALAIFRNCQAKRALLGAVAFCVNRGH"
     gene            11135..11368
                     /locus_tag="VF226_20085"
     CDS             11135..11368
                     /locus_tag="VF226_20085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7076343.1"
                     /translation="MREILRSNNPVLLSFVEVLLRDAGVQPLVADGNMSIMEGSIGVL
                     PRRILVSTDDLTRARKVLQEAGLGHWVNDDDPN"
     gene            11352..12140
                     /locus_tag="VF226_20090"
     CDS             11352..12140
                     /locus_tag="VF226_20090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7076344.1"
                     /translation="MTTRTDDVGTSEITEDAFLGGALTVLQPRQGYRAGIDAVLLAAA
                     APVGGKPGERVLDAGAGVGVVGLCIAQRAPGTRVTLVENEPELIVLARENVIRNGLEE
                     RVSVIAADITAPAAVLAAAGLSADSFDHVVANPPFHIEGHGRRPRHVIKAAAHAMPAG
                     SLAQWVRFLTRVAAPGGSLTMVHRPDVLPDLLGLLRSRFGGLKILPLHPRVGEPAVRI
                     LVQGRKGSRAPLTLLSGLVLHEKDNRFRPEVARILRDGAALPLD"
     gene            12229..13080
                     /locus_tag="VF226_20095"
     CDS             12229..13080
                     /locus_tag="VF226_20095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX7076345.1"
                     /translation="MRFFKRRPVVPVIRFSGPIGFATPLRPGLSIGAVASGLEKAFSL
                     SKLPCVAVTVNSPGGSPVQSNLIFKRVRQLAEEKKKKVYVFCEDVAASGGYYLAVAGD
                     EIYADPSSIVGSIGVISASFGFNKAIERLGIERRVYTAGPAKGLLDPFQPEKPDEVER
                     LKAIQRDVHDVFIGVVKDRRGARLRGHDAELFSGAFWSAAKALELGLIDGVSDLRTKM
                     RELHGDDVQLKVVPFGPGGWLSRLLRFPRLAPAGMDSLANGLPLSLGDDLLSAIETRS
                     LWSRYGL"
     gene            13128..13328
                     /locus_tag="VF226_20100"
     CDS             13128..13328
                     /locus_tag="VF226_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076346.1"
                     /translation="MPQVIALLLAGAGLYAGCRWVSREVRRALATAQEAEERLRQQSA
                     PKDLGSLEWDAEAGVYRPSKQA"
     gene            13446..14408
                     /locus_tag="VF226_20105"
     CDS             13446..14408
                     /locus_tag="VF226_20105"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX7076347.1"
                     /translation="MATRTAIRPATEPLGQTVSGLQPTRSFQDLILTLQQYWSEQGCA
                     ILQPYDMEVGAGTFHPATTLRALGPKSWNVAYVQPSRRPKDGRYGENPNRLQHYYQFQ
                     VIMKPSPPDIIDLYLESLARIGLSREIHDIRFVEDDWESPTLGAWGLGWEVWCDGMEV
                     TQFTYFQQVGGFDCNPVSVEITYGLERLAMYVQGVDRVFDVSFNGREDERKLTYGDVF
                     LQAEQEYSRYNFEYADTDMLFQHFRDAEKECLALLEKGESEDGRHLLALPAYDQCIKA
                     SHIFNLLDARGVISVTERQSYILRVRELAKGCCAAWLKTEGGGA"
     gene            14405..15004
                     /locus_tag="VF226_20110"
     CDS             14405..15004
                     /locus_tag="VF226_20110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX7076348.1"
                     /translation="MSRPKTLLEMAGADLSPGALAQSVVIIIDAQNEYLDGKLALPGI
                     KPALDAISTLLAKARDAGTPVIHIAHKGRPGGLFDREGHGGAIIAAAAPATGEPVIEK
                     PLPNAFAQTALDSELKALGRKSLIMAGFMTHMCVSSTARAALDLGYRVTIAGDAAATR
                     DLPDPFGGVVPADALHRAALAELGDRFAIIATVDQIPKD"
     gene            complement(15014..15454)
                     /locus_tag="VF226_20115"
     CDS             complement(15014..15454)
                     /locus_tag="VF226_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7076349.1"
                     /translation="MDTVIRAAVIYAFLFLVFRVAGKRSLADITTFDFILLLIIAETT
                     QQALIGDDFSIITAILLISTLFLIEVVLTLLKQRYPGVDRLMEGEPVVLVENGRPLQE
                     RLDLERVDLDDILEAARRWRGLERIDQIKFAVLERDGNITIVPR"
     gene            complement(15467..15973)
                     /locus_tag="VF226_20120"
     CDS             complement(15467..15973)
                     /locus_tag="VF226_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076350.1"
                     /translation="MDKARTEDLHGLTAASQMARMRWKWRLQHLGWAVIGLLVLAGAV
                     GLFGSGPLSSTTRAANELTLEYDRFIRRGTPFTLKATIPVPPGSTRAELSLPRDYLGS
                     LRVLSIVPEPIQVVSGAELTKFLFAADPASDLLVVDLHAEPIKAGMLDAELIAGGARL
                     QFTQFVWP"
     gene            16119..18218
                     /gene="glyS"
                     /locus_tag="VF226_20125"
     CDS             16119..18218
                     /gene="glyS"
                     /locus_tag="VF226_20125"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX7076351.1"
                     /translation="MPELLLELFSEEIPARMQPRAAEELKRLVLGALKDQGLAVGEAQ
                     AFATPRRLTLIARDVPARSPDQSEERKGPRVNAPEQAIQGFLKAAGLSSIGEASVVSD
                     PKKGDYYVARIERPGRSAYEVIAEAVPDVLHRFPWPKSMRWGRYSFSWVRPLHGVVCL
                     LDGEIVPFEVAGVASGNTTRGHRFHGNVPFRVRGIDDYLQKLSANRVILDATERMQRI
                     EHQAKELAAKHKLQLVPDHGLLAENAGLTEWPVVLMGHFDEAFLELPPEVIITSLRTH
                     QKCFALRDPKTGNLANRFLLVANLEASDGGATIVAGNEKVIRARLSDARFFWEQDLKR
                     PLDEMAHALRGVTFHEKLGTQWDRVERIAELSYQIAGAVDAAPEAARRAAQLAKADLV
                     SGMVGEFPELQGLMGRYYAEAAGAKPEIARAIELHYKPKGPNDTVPSAEDGDCVAIAV
                     ALADKIDTLVGFWRINEKPTGSGDPYQLRRAALGVIRIILENDLRVSLIKRISAAHAE
                     YDKQGQRAVKGSEEEITADLLDFFADRLKVHLREKGARHDLIDAVFALPGQDDLALIV
                     KRVEALGKFLETDDGANLLAGVKRAASILAIEEKKDKTSYDGDYDLKLLEAPEELSLA
                     AAIEATKQDTRAAIAVENFEGAMRALADLRRPVDAFFDKVTVNVEDQRLRQNRLRLLS
                     QIRAATMTVADFSKIAG"
     gene            18225..18638
                     /locus_tag="VF226_20130"
     CDS             18225..18638
                     /locus_tag="VF226_20130"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX7076352.1"
                     /translation="MLQHLFVYGTLMSTTGHRMALRLAREGRLVGPAAVRGRLYDLGA
                     YPALVEAEAEDGEVFGEVHALTDPAASFRWLDAYEGIVPGRDCEYVRVQRSVRLETGA
                     ELTAWLYLYRAPVAGRRLIESGRWMSRFASAGGVR"
     gene            complement(18961..19737)
                     /locus_tag="VF226_20135"
     CDS             complement(18961..19737)
                     /locus_tag="VF226_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7076353.1"
                     /translation="MTTFDPKAAAAFIAEAHRSRAPYRNLPAEIAPPTVADAYAAQEA
                     LCELWSPIYGPVRGLKIATTTKVMQQLMGINHPCGGMIYERRIHQSPARLRREDFINL
                     MVEFELAVRLGADLPRRDVPYTAADVRPAVAEAMPAFELIEDRGADYKSTSALSLIAD
                     NAWNGGIVLGAPQRVPADMELDGIRGHLTVNGAERGTGVTDDPMGALAWIANLAADRG
                     RPLKEGMVVITGSVLPTIPIAAGDTFVFSLDGIGSTELSV"
     gene            complement(19748..20356)
                     /locus_tag="VF226_20140"
     CDS             complement(19748..20356)
                     /locus_tag="VF226_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7076354.1"
                     /translation="MMKLLSSPASPYGRKVSIVAAMKGVADRIEKRRVDTYPLINEEL
                     RRENPLSKIPILILEDGTHVYDSPVICEYLDSLTPDPRLFPAAGPERFRTLTLAAMAD
                     GILDAALLLVYEKRYRPEDKWVDTWMQRQQLKVDTTLDWFERSLSELAPKPDYAHVTL
                     ACALGYLDFRHGGTWRAGHPNLVAWLDAFDKAVPAFAETRPD"
     gene            complement(20463..20828)
                     /locus_tag="VF226_20145"
     CDS             complement(20463..20828)
                     /locus_tag="VF226_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076355.1"
                     /translation="MADQLLWLETLLRFSSGLVLLIMPLTTARILGLPLPQALLWPRL
                     LGALLIGMAAAILLEGSIAGGKGLGLGGLVLINLIGAAVIITLLVMERGSQTRRGKLV
                     LWGLAVGLIMLGLLEIAAA"
     gene            complement(20861..21427)
                     /locus_tag="VF226_20150"
     CDS             complement(20861..21427)
                     /locus_tag="VF226_20150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076356.1"
                     /translation="MPNRMPFSMLAATALLLAGCSGSGSLTTAALTGGGGQETAQAAA
                     ASSAPAGPPPSDPTSRAVQVGATSARAARCGFYFDPEKLKANFLAAEMAQGAGPEQLQ
                     KIEREYEYVRLSVAKKIADEADYCDKLKTKEIKEDLNRHLAGDFTPRPKKEEPQQGLF
                     AGLIDDAPKKKWKPDEFFDDLGRSPAQR"
     gene            complement(21565..22323)
                     /locus_tag="VF226_20155"
     CDS             complement(21565..22323)
                     /locus_tag="VF226_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020812142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7076357.1"
                     /translation="MDTILPLLTDPAAWVALITLIAMEVVLGIDNLIFIAILTNKLPE
                     NRRDMARKIGISAALVLRLALLFTIAAIIRLTTPVVSIFGLDFSWRDMILIAGGLFLV
                     WKATKEIHHRVDPDPAPDIFDRRTVALGTGSAIVQILLLDIVFSIDSIITAVGMTPHV
                     PIMVVAVIVAVMVMLLAATPLANFINANPTIVMLALSFLLMIGMVLIADGFGVHVPKG
                     YVYAAMAFSALVEALNMMARRARQREKAAGESAH"
     gene            22628..23725
                     /locus_tag="VF226_20160"
     CDS             22628..23725
                     /locus_tag="VF226_20160"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7076358.1"
                     /translation="MKSSNLWLVASCVVVAGASAAAVLALDHAGIISLTPSAEQPAEQ
                     VPVRLVETVPVAPARQTIPVEPAPEPEERLAYLQPETAVLPPPPAPEEKSEADELPSS
                     EPAEPEVPDSWSAEVQPEETEISDEAQLLDGPSPASPPRFSKRRLTSHSAALTKRLAE
                     ISPGANARLAKKFAAAEAPWPPHEIALVAIKDEKSLELFARPEDGEWKFIHRYPVLAA
                     SGGPGPKLRQGDRQVPEGIYKISFLNPNSRYHVSLRVNYPNAFDRQMAEAEGRTRLGG
                     DIMIHGKAVSAGCLAVGDPAAEELFVLAAQVGLENIRLIIAPTDFRRRGLPAIEPGQP
                     NWLPKLYTEVAAAMSEFKSPPRIGLLSFFGN"
     gene            23813..24847
                     /locus_tag="VF226_20165"
     CDS             23813..24847
                     /locus_tag="VF226_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7076359.1"
                     /translation="MLTRRTGDGPLLVDLALQGGGAHGAFTWGVLDRLLEEPDIQIDG
                     ISGTSAGAMNAAVLADGYAAGWREGARTALEEFWNQVSCAAMLSPLRRSPMDILMGRW
                     TMDTSPAFITMDLMARLFSPYDLNPHGANPLREILTRCIDPEHLVNGPIRLFVTATNV
                     STGRGRVFRNAELTPDVLLASACLPTLFQAVEIDGEAYWDGGFSGNPTITPLVRECAA
                     SDTVIVQVNPIERSGTPRSAREIINRVNEVSFNATLIKELRMIALLRQVADPGDCEGK
                     LWAEMRIHRIASPAMAELGYSSKLNAEWDFLLALRDEGRRSADAFLKTHGAALGKRST
                     LDLDPLLEQV"
     gene            24854..26308
                     /locus_tag="VF226_20170"
     CDS             24854..26308
                     /locus_tag="VF226_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEX7076360.1"
                     /translation="MGLLGILVGLIFLVWLAYRGWSVLLLAPAASMIAAVAAGEPLLA
                     HWTQTFMGAAAQFLAQYFPLFLLGALLGKLMDDSGSIAAIARYLQERFGPERAVLAVV
                     IAGAVVTYGGVSLFVAFFVLVPMAIALFRAGDLPHRLIPAAIILGTATFTMSALPGTP
                     AIQNAIPMPFFGTTPFAAPGLGLIASAIMLLFGLWWLGLKVAAARRAGEGYDAPVEEI
                     AKGAADDEFIRERATVAREFDPAEIHHGCHSASEPPVAVAVAPLVVVIAVNVLMSFVV
                     LPRLDLAFLAEERWGATSPAGVAGVWSVITALTAAVATVILLNRQRLPGLRETMDAGA
                     NASVLPALSVASLVGFGAVVAALPAFDAVRDWVLAIEGGPLVSLAVSTNILASLTGSA
                     SGGLSIALGALGENFMRLAADYGIEPALMHRVSVIGAGTLDVLPHNGAVVSLLALCGA
                     THRTSYLDLAIVAVGGALLGLIAIIVLGSRFGAF"
     gene            26419..27021
                     /locus_tag="VF226_20175"
     CDS             26419..27021
                     /locus_tag="VF226_20175"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEX7076361.1"
                     /translation="MAQQATQADFDALLGETRRDVARHWGWFLALGILLVIVGMVAIA
                     FPFLSTIAAKIALGWILLIAGIGAVIHSFYVKRWGGFLWNLLIGLLYLIVGFWLAFFP
                     LTGILTLTIVLAALFIADGIMEIIMGFRVRPHEGWGWLIFSGVIAILAGLLIALNLPS
                     SAVWALGLLAGVKLLVSGWSFIFLALTGKSMSEQTATTAT"
     gene            27105..28121
                     /locus_tag="VF226_20180"
     CDS             27105..28121
                     /locus_tag="VF226_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX7076362.1"
                     /translation="MSSLRLLLKPLGWLGAGLAILIRVLLVAWATLAIYYSNLPWPAL
                     RVVLAIAFAAFATWAIWFSRQRRAHGALAVLYLGVILWWLAIPPSHDRHWRPEVAVMP
                     RAIVDGDRVHLTNVRNFVFRTADDFTVRYEERDVRLSMLTGIDFYISFWAKGPVGHTF
                     LSFTFADAPPLSISIETRPEVGEDFHPLASLFKQFELIYVVGDERDIVGLRTNHRDES
                     VFLYRLNTSPENARRLFLVYLDRINELADQPEWYHLFSNNCTLNIIRYANAAGRRGRF
                     DIRHLLNGLIDSYLYYSGRLDTSLPLDELRRLSRIDEAAQVADNSPDFSERIRAALPS
                     VTRR"
     gene            complement(28167..>28568)
                     /locus_tag="VF226_20185"
     CDS             complement(28167..>28568)
                     /locus_tag="VF226_20185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7076363.1"
                     /translation="GVGDRVTFEVAKADTYPARGYDLICFFDCLHDMGRPVDAVRHAI
                     KAVAKDGTLMLIEPFARDRVEENINPIGRLYYAASTTMCCAHAISENGTHVLGAQAGQ
                     RRLEDVLKEAGLKTIRKAVETPFNLILEARL"
BASE COUNT         5011 a         9112 c         9328 g         5117 t
ORIGIN      
        1 gacgggccga agctcgtcga tgtccacgcc gggccgctgc ctgaacagcc gtccctcatc
       61 gtcctgatgc ttcagcagcg caccatggcg cagatgatcg agcggcagct gacccatcgg
      121 gccgcggcaa gatccatctc gggcatggcg gcgacgctgg cccacgagat caagaatcca
      181 ctgtcaggaa ttcggggcgc cgcgcaactg ctggagccga gccttagtcc tgcagacaga
      241 tcgctggcgc agctgatctg cacggagacc gatcgcatcc gcaatctggt cgaccggatg
      301 gaggtgttcg gagacgagcg cccgctggcc aaggagccgg tcaacatcca cgacgtgctc
      361 gatcacgttt gccagcttgc cggcaccggc ttcggccgcg gcgttacgat cataccgcgc
      421 tacgacccct ccctgccgcc cgttccggcc aatcgcgaca agctgatcca ggcctttctc
      481 aacctcgtga agaatgccgt ggaagccgtc gagggaaacg gcgagagcgg gcggctcatt
      541 ctgacgacgg cctttcgccc gggagtccgg ctttcggtgc cgggaagcgg cgcccgggtc
      601 agcctccccc tgatgatcga ggtggaggac aacgggccgg gcgtgccgga ccacctcaag
      661 ccatatctgt tcgacccgtt cgtcaccacg aagcgcagcg gctccggatt gggcctcgcc
      721 ctggtcgcca agatcatcgg tgaccacggc ggcatcatcg aatgcgagtc cgagccgcgc
      781 cgcaccattt tccgcgttct gctgccgatg caggacaggc ctccccagac cagtcaatcg
      841 gattagagcc atggcccgcg gcaccattct cattgccgat gacgacagcg cgatccgcac
      901 cgtgctcaac caggcactcg cccgcgccgg atacctcccg cgcgcgaccg gcaacgcggc
      961 cacgctctgg cgctgggtgt cgcagggaga aggcgatgcg gtcatcaccg acgtcgtgat
     1021 gccggacgag aacgccttcg acctgatccc gcgaatcaag aagctgcggc cggagctgcc
     1081 gatcatcgtc atgagcgccc agaacacgct gatgacggcg ctgtcggctg ccgagaaggg
     1141 ggctttcgaa tacctgccga agcccttcga cctgaacgag ctcgtcagca tcgtcggccg
     1201 ggctctggcg gagccgagtc ggagtgcggc caagagtttg cccgagaacg agcccgacga
     1261 tctgccgctg atcggccggt cgcccgcgat gcaggagatc taccggatca tcgcgcgcct
     1321 gacccagacg gacctcaccg tgatgatctc cggcgaatcg gggaccggta aggagctggt
     1381 cgcacgcgtg ctgcacgact tcgggcgccg gcgcagcggg ccgttcgtcg ccatcaacat
     1441 ggctgccatt ccccgggagc tgatcgagag cgagctgttc ggccacgaga agggcgcgtt
     1501 cacgggcgct catacgcgta tgccggggcg cttcgagcag gcggaaggcg gcacgctgtt
     1561 tctcgacgag atcggcgaca tgcctatgga agcgcagacg cggttgctgc gcgtgttgca
     1621 ggagggtgag tacacgaccg tcggcggacg tattccgctg cgcaccgacg tgcgcatcat
     1681 cgccgcgacg caccgcgagc tgaaaagcca gatccagcag gggctgttcc gcgaggacct
     1741 ctactaccgc ctcaacgtcg tcccgctgag attgccgccc ttgcgcgagc ggctgggcga
     1801 catcgacgac ctcgtgcgcc attttctccg gcaggcggcc cgcagcggcc atggccagaa
     1861 ggtcgtggaa gcggcggcgt tggagcggct gaagcggcac acctggcccg gtaacgtcag
     1921 ggagctggag aacctggtac gccggctcgt cgccctctat ccgcaggata cgatcaccgc
     1981 ggaaatcgtg gacgctgaac tcggccaggc gctcacgtcg ccgcgcatcg acgaagggcg
     2041 ccccgacgaa aacgcttcgc tttcggagct cgtggaaaac taccttggcc gctacttcgc
     2101 ccggcatggg cgcgatctgc cgccgcctgg gctctatgac cgcgtcatcc gcgaagtgga
     2161 gaagccgctg atcacggcag ctttggcggc gacccgcggg aatcagattc gggctgcaga
     2221 gctcctcggc ctcaatcgca acaccttgcg cacgcgcatc aaggacttgg acattcaggt
     2281 attcaagaca cccggcatgg catgagaaac gcccacgcac agggccaagc gattgccttg
     2341 acttgagcgg aggatatcga cgatcatgtc gcgaaaatgt cacagtcttg tagctgatct
     2401 gtgatcgcat cgcttcacgc cgatgccacg aatgtaaccg aatagctacg tatctggcta
     2461 aagagcagac cgaccggcgg ccacggcggg ttcatgcgag tgcgcttgag gccgatgaga
     2521 ggcgttgtgg atgacgtccg ctgacccgcg aagggcgcca ggcgacatcg tggatgaggg
     2581 ccggttgccg tttcccgagg cgaacccgct cgccttctgg atcggcttcg tcgtcgtcat
     2641 cctgtcgctc gtctcggggt ttgcgaccta tctcatcctg accgggctca cgccgatcgt
     2701 gcctcgggac gacatcgtcc tgacggtgct gttcatcaac gtcgttctga tcctggccat
     2761 gttcgccgtc atcggctggc aggcgatcgg gcttgccaag gcgtggcggg acaaggaggc
     2821 cggcgccagg cttcacgtcc gcatcgtcgg tatgttcagc atcattgcgg cgctgccggc
     2881 cattttgctc gcgatcggag caacgacgac cttctcgcgc tctctggaca actggttctc
     2941 gacgcggact cgccagatca tcgccaactc gctcgacgtg gccaacgcct acctcgagga
     3001 gcatgggcag gtcatccgca ctgacatcgt caacatggcg aaggatctcg acgatgcggc
     3061 cgatacgctc cgtgatgatc ccgagcagtt ccgtacccag gtgattgcgc aagcaggctt
     3121 gcgtgatctt ccggccgcgt acgtcattga tgctgaggga cggccgatca tcagcgccgt
     3181 cgaggattcg cgcttgccct acgtcccgcc gcccccgcag gtcatcgccc acgccgaggc
     3241 ggggcaggtg ccgctgctca tgccgtccaa cagctaccgg gtggcggcga tcaccaagct
     3301 caacgcgtat ccgggctcct atctttacgt tctgcggggt gtcagcccca aggtgatggg
     3361 ttatctgcgg caaacgcagg cgggcgttgc cgagtacgag gagctgcgaa aacggcgcgg
     3421 cgggctcaag atcgcgcacg ggctcatgta cttcatgatc tcgatgacgg cgctgctggc
     3481 cgccatctgg gccgggctgt ggttcgccgg ccgcttcgtg gcgcccatcc gccgcctgat
     3541 cgccggtgct caggaggtct cccgcggcaa cctcaacgtc acgctgccgg tccggcgcgg
     3601 cgaaggcgat ctgaggcgcc tgtcgcagac cttcaatacg atgaccgctg agctgaagat
     3661 gcagcgcgat gcgctcgtct cggccaacga gcagcttctc gagcggcggc gtttcatcga
     3721 ggccgtgctg acgggtgtgt cggccggcgt cctggggctt gatcgcgagg gccgtatcag
     3781 gctcgccaat cggtccgcgg agcagttgct tggggcgggc gcggagaacc ttgtggggcg
     3841 ccgtctcgag gatgccgtgc ccgagatcgg cgagctgctg accaaggcgg aggaaaagcg
     3901 cctcgcgtac aggcttcagc atcaagtcaa gctcatcaac aacaacggtg atgagcgcat
     3961 cttggccgta cggttcacgc gtgaggtgga gggcggagaa gagcagggca cggtcgtaac
     4021 cgtggacgac atcacggagc tcgtttccgc ccagcgtacg tcggcctggg ccgatgtcgc
     4081 ccgccgtatc gcccacgaga tcaagaaccc gctgaccccg atcatccttt ccgccgagcg
     4141 catccgccgc aagtacggca aggtgattac ggacgaccgg gagaccttcg acaagctcac
     4201 cgcgaccatc gagcgccagg ccggcgacat caagagcatg gtggacgagt tcgcctcctt
     4261 cgcccgcgtg ccgaagcccg tgatggagcc cggcgatttg cgcgacgtgg tccaggagcc
     4321 ggtgatcctg tttcgtgaaa gccacccgca tatcgcctac gagctcgatt tgccggcgga
     4381 gccgctgatg ggcctcttcg accggcgtct catcacccag gcggtgacga acctcatcaa
     4441 gaatgcgacc gaagcagtcg aatcggccgc cgaggcgccc gagccggact ggagcggccg
     4501 catcgaagcc atcttgcgct cagtcgacgg gcgaatcgaa atcgaggtca tagacaacgg
     4561 catcggcctg ccgaagcaga accgatcgcg tctgctggag ccctatgtca ccaacaaggg
     4621 caacaaaggc accggcctcg gcctcgctat tgtccagaag atcgtcgagc agcatgacgg
     4681 tatgctgacc ctggaggatg ctccgccagg tccgcagcgc agccggggtg cgctcgtgcg
     4741 catcacgctg cctgtccgca acgagttcac aagcaagacc gatgtcaaat ccgaagcagc
     4801 cgagtaggtc tgcctcggtg gcatcgagcc gccaagggaa gggagtgacc gatggcgtct
     4861 gacatccttg ttgtcgacga cgaagccgac attcgcgagc tcgtttccgg gatcctcgag
     4921 gacgagggcc accgctgccg gctcgcgagg gatagcgacg aggccctgcg cgcaatcgag
     4981 gagcgccggc cgcacctcgt tattctcgac atctggctgc agggcagccg gctcgacggg
     5041 ctcgaggtgc tgtcgatcat caagaaggtg caccccgacc tgccggtggt catcatctcc
     5101 ggccacggca acatcgagac ggccgtcacc gccatcaagc gcggcgccta tgactacatc
     5161 gagaagccgt tcaaggccga ccggctgatc ctggtgacga tgcgcgcgct ggaggcctac
     5221 aaccttcgcc gcgaggtgaa gcatctgaag gagcggtcga cgcagagcgc ggagatgatc
     5281 ggccgctcgc cggccatcaa ccagctccga catcagatcg atcgcgtcgc gccgaccaac
     5341 agccgtgtgc tgatccgcgg cgcatcgggt gccggcaagg agctcgccgc acgggtcctg
     5401 cactccaagt cgcaccgcgc caagggtccc ttcgtcgtgc tcaatgcagc ggccatggtg
     5461 cccgaccgcg tcgaggaaga gctgttcggc accgaggacc gcaccggcga ggcgcgcaag
     5521 gtcggcgccc tcgaggaggc ccataccggc acgctctaca tcgacgaggt ggcagacatg
     5581 ccgctcgaga cgcagggcaa gatcctgcgc gtgctggtcg agcagaagtt tctgcggctc
     5641 ggcgggacgc acaaggtttc cgtcgatgtg cgcatcatct cctcgacgag ccgcgatctc
     5701 gagcgcgaaa tggccgaggg gcgcttccgc gaggacctct atcatcgcct caacgtggtg
     5761 ccgttgagag tgccgggcct cgccgagcgg cgcgaggaca ttcccgagct cgtcaactac
     5821 ttcgtgcagc agatcgcgca gagctcggga ctcgcgccgc gccgtatcgg cgaggatgcc
     5881 atcgccgtcc tgcaggccca cgactggccg ggcaacgtgc gcgagctgcg caacaacatc
     5941 gagcgcctga tgatcctctg cggcggggat cccgacgcgg tcatcacggc cgacatgctg
     6001 ccggaggaga tcggctccaa cgtgccgctg cccgtgaatg ggggcgcgga gcacctgatg
     6061 tcgctgccgc tgcgcgaggc gcgcgagatc ttcgagcgcg agtacctgct ggcccagatc
     6121 aaccgcttcg gcggcaacat ctcgcggacg gccgagttcg tcggtatgga gcgctcggcc
     6181 cttcaccgga aattgagggc gcttggcgtc agctccgatc accgcagcgg ggtgacagcc
     6241 ggccagccct gatgcggtga tcgagaatta gacggcgttg actgctgagg tccgccgagt
     6301 tttcttggtc agatcgtatt ggcgacgtct gtgtcgcgtt gcccgttctg ctttcgaggc
     6361 ttccccgcga ggggagcttc ggaacgggga cgccagggcg ccagcgtgcg ttgacaggga
     6421 acgatagcgg ggcagccgag cgcgattccg ttcgacccgc ccttatccga acgaggccct
     6481 atcgacatga aagtgatcat ctgcggtgca ggtgtcgtgg gtctcggcat cgccgagcgg
     6541 ctcgctgccg agggcaacga cgtctcggtc atcgatatcg atccggccct cgtgcagcgc
     6601 gccaacgacg tgctggaggt gcgcgcgacg ctcggcaatg gcgctcaccc ggacgtgctc
     6661 gagcgtgccg gcgcgcgtga cgccgacatg ctcatcgccg tcaccctggc cgacgaagtg
     6721 aacatgatcg cctgccaggt ggcacacagc ctgttcgaca tccccaccaa aatcgcccgt
     6781 gtccgcgcgc agagctacct ggccaaggaa tatgccgcgc tgttcgcgcg caattgcctg
     6841 gccatcgact tcatcatctc gcctgaggtg gaggtcggca acatggtgct gcgccggctg
     6901 cagcttcctg gtgcgttcga ggcgctgagc ttcgccgatg ggcgcgtgct tgcggtcggc
     6961 atcgcctgcg aggaggactg cccggtcgtc gatacgccca tccggcagtt gagcgagctc
     7021 tttcccgacc tgcccgccgt catcgtggcc gtcgtccgta agggcaattt gttcgtgccg
     7081 cgcctggacg aacagctcaa ggccggcgat gacgtctaca ttgttgcccc cgccgaccag
     7141 gttgcgcgca cgctcaagat cttcggtcac gaggagcagg ttgcgcggcg ggtgatcatc
     7201 gccggtggcg ggaacatcgg cctctatgtc gcccgcgaga tcgaggcgcg cgagcatatg
     7261 gtgcgggcca agatcatcga ggcgaaccgc ggccgagcga tgcagatcgc agaggagctc
     7321 aaccgcaccg tggttctgca cgggagctcg ctttccgagg agctactccg cgaggccgag
     7381 gcccaatcgg ccgacacgat ggtagccgtc accaacgacg accaggtcaa catcctgacg
     7441 tcggtgctgg cgaagcatct cggctgcaaa cgcagcctgg ctctcgtcaa ctcggccggc
     7501 tatacgccta tggtgcgctc gcttggcatc gacgcgcaaa tcaacccccg cacgatcacc
     7561 gtctcgcgca tccttcagtt cgtccggcgc ggccgtatcc gtatcgtcca ttcgatccac
     7621 gacggggcgg gggagctcat cgaggccgag gcgatcgaga cggctccgat catcggcaag
     7681 tcgatccgcg agatcgacat gtcggagggc gtgcgtttcg gcgccatcgt gcgcaatggc
     7741 caggttttcg cgccgacagg cagcacggag ctggagccca aggatcgcgt cattctgttc
     7801 gtgcgcgcgg atcacgtccg gcaggtcgag cagctgttcc gcgtcagccc cgattacttt
     7861 tgacaggggg tacagcggtt cccattgcgc tctccgatgc acctgctaaa acccgcatgt
     7921 gcgcacttcg cagccggcag agatgattca tgatggccca ggcgccatcg gagtgctgat
     7981 tccaccaagg gtttcaggta agctgatccg aggtggacgg agcgccaccg aaaaacataa
     8041 ccgaacaaaa acggataaga agaaatggct gaacgagcac aaagcctgca ggacaccttc
     8101 ctgaaccacg tccgcaagaa caagacgccc ctgacgatct tcctcgtcaa cggcgtgaag
     8161 ctgcagggta tcgtgacctg gttcgataat ttctgtgtcc tgctgcggcg cgatggacac
     8221 tcgcaactgg tctacaagca cgccatatcg acgatcatgc cgggccagcc cgtccacctg
     8281 atggagcagg agccggcgga agcgcagcag ggatagacgt tcgattagcc gcgcagacga
     8341 gaccaagacg agacgcaggc gccggggcgg caatggttcc gaaccaacgg cgatcacgcg
     8401 tgctgtcgtc ctcgttccag tgctacagac gacaggcagc ccggggacgg ccaagcggtc
     8461 ccgggctggc aatggcacgc gcggcgccac gctccattct ccccaggatc ggctggccga
     8521 ggctgccggg cttgccgaag ccatcggtct cgaagtccgg gccaagctca tcgttccggt
     8581 ggcgaggccg cagccggcga cgctgttcgg ctccggtaag gtcgaggagc tgaagcaact
     8641 cgttgccgag cacgaggccg gtctcgtcgt catcgaccac gcggtctcgc ccatccagca
     8701 gcgcaacctc gagcgggcgt ggaacgcaaa ggttctggac cgcacggggc tcattctgga
     8761 gatcttcggg cgtcgggccc ggacccggga agggcgcctg caggtggagc tcgcccacct
     8821 cacctaccag aagagccgcc tggtgcggag ctggacccac ctcgagcgcc agcgtggcgg
     8881 ctacgggttc ctcggcggtc ctggtgagac ccagatagag acggaccggc ggctcatcca
     8941 ggagcgcatc gacgccatcc gcaaggaact ggacgtcgtc gtgcgcacgc gcgaattgca
     9001 ccgctccgga cggcgtcggg tgccatatcc gctggtcgcc atcgtcggct acacgaacgc
     9061 tggcaagtcc acgctcttca acaagctgac cggcgccggc gtggtcgcga tggatcaggt
     9121 cttcgccacg ctggacccga ccatgcggga gctgcggctg ccgagcggtc gcagggtcat
     9181 cctgtcggac actgtcgggt tcatttccga cctgccgacc tcgctcgtcg ccgccttccg
     9241 cgctacgctc gaggaagtga tcgaggccga tctcatcctg catgtccgcg atatcagcca
     9301 tcctgaaacg caagcgcaaa gggcggacgt ggaaagggtt ctgcgcgagc tcgggatcga
     9361 ggtggatcgg ccggacgggc tcatcctcga ggtctggaac aagatcgact tgctgccgcc
     9421 ggaatcgctg gaggagctgc agcatgcggc cgagcgcgcc cagcagaagc cgctgctcgt
     9481 ttcggccgtc gaggagaggg gcctcgacgc cctgctggaa gccatcgatg cccagctcgg
     9541 ccgcgccgac gaggtgctga ccatcgccgt tcccgcgacg gcaggcaatc tgcttgcctg
     9601 gctgcacgag aacgcccagg ttctcgcccg agagacggcc gaaactggcc agaccatctg
     9661 ccaggtgcgc atcgcgcgcg cgaaaaaagg ccgcctgttc agccggttgc ggcagaacgg
     9721 gctggccgtt gcgagctgac gggcagggtt gccgaggctg tcatcaagga tctaaccgcg
     9781 agctcgtggt tacgcatcgt ggatgcccac cttcgtgggc atgacggtct atgtatatcg
     9841 gccgcccgga aagccatcga tcaagggaag aatcgcagac ctcctcagcg tcatacccgc
     9901 gcaggcgggt atccgcgatg cgcagcagca aaattgccgc tgtagctgtg ctcggcctct
     9961 cagggacgac aggcgggggt gatggctcag cgccgctcag tgcccccggt tcacacagaa
    10021 cgccacggca cccaggaggg cgcgcttggc ctggcaattg cggaaaatgg cgagcgcgtc
    10081 ctcggccatc gagccgtaat agcgagcccg ctcgagcgtc gcctcgatcg ccttatgccg
    10141 gcgcatcaag ctcatggcgt gctcgaaatc gccttcgcgg atatctcctt cgcctaatgt
    10201 tcgccgccag aacgcccgtt cctcatctga accgcggcgg aaggctagga tgacgggcag
    10261 cgtaatcttc ccctcgcgga aatcgtcacc caccgacttg ccgagccgcg cgctgtcgcc
    10321 cgaataatcg agcgcgtcgt cgacgagctg gaaagcgatg ccgagggtct tgccgtagga
    10381 cttgagggct gcctgctcgt ctgccggtcg gttggcgatg gcgccgccga cctcggtcgc
    10441 agcggaaaac agggctgccg tcttggcatt gatgatcgcc aggtattcgt cctccgtcgt
    10501 ggcggtgttc ttggccgcgg cgagctgcat cacttcgcct tcggcgatca cggcggccgc
    10561 gttggaaagg attttcaacg cctgcagaga gccgacctcg accatcatgc ggaaggcctg
    10621 ccccagcagg aaatctccga ccagcacgct cgcctgattg ccccagacga gccgcgcggc
    10681 ctttttgccg cggcggaagt cgctctcgtc gacgacgtcg tcatgtagta gcgtcgccgt
    10741 gtgcatgaac tctacggcag cggccagcgg cacgtgacca tcacccgtgt agccgcacag
    10801 cttggcggcg gcgatggtga gcatcgggcg catacgcttg ccgcccgaat cgatgagatg
    10861 ttgggcaagc tgcggaataa gctcgacgtc ggagactgcc ttgtccagaa tgagccggtt
    10921 caccgcgccc atgtcctcgg ctgcgagctc gagaataggg agcagactgt gcgtcgggtc
    10981 acgcgtttca tcgattggaa caacgacccc cactgggctc ttctcctgga tgattctctg
    11041 atcgcggtga tcttaaccac gggttccgga accattttca cggcagattg ccgcatggtt
    11101 aactccggaa aagactgcag aaaaggtgtc catcatgcgg gagattctgc gctcgaacaa
    11161 cccggtgttg ctgagcttcg ttgaggttct gctgcgcgat gccggggttc agccactcgt
    11221 cgcagacgga aacatgagca ttatggaagg ctcgatcggc gtactgccgc gacgtattct
    11281 cgtaagcact gacgatctta cgcgtgcgcg caaggtgctg caggaagcgg gcctcggcca
    11341 ctgggtcaat gatgacgacc cgaactgacg atgtcggcac gagtgagatt actgaggatg
    11401 cgtttctcgg cggtgctctg accgtcctgc agccgcggca aggctatcgt gccggcatcg
    11461 atgcagttct tctggcagcc gctgccccgg tcgggggaaa gcccggtgag cgagtgctcg
    11521 atgcgggcgc gggtgtcggc gtcgtcggac tatgcatcgc ccagcgggct cctggcacgc
    11581 gcgttacgct ggtcgaaaac gagccggagc tcattgtgct cgcgcgcgag aacgtgatcc
    11641 gtaacgggct cgaagagcgg gtcagcgtca tcgcagcgga catcacggcg ccggctgcag
    11701 tgttggccgc tgcggggctt tcggcagatt cgttcgacca cgtcgtagcc aatccgccct
    11761 ttcatatcga agggcacggg cgccggcccc gccatgtgat caaggcggca gcccatgcca
    11821 tgccggcggg cagtcttgcc cagtgggtcc ggtttctgac gcgcgtggca gcccccggcg
    11881 gcagcctgac catggtgcac cggccggatg tcctgcccga cctcctgggg ctgctgagga
    11941 gccggttcgg tgggctgaag attttgccgc tccacccgcg cgttggggag ccggccgtac
    12001 gcattctcgt gcaggggcgg aaagggagcc gtgccccgct cacccttctg tcggggcttg
    12061 tgctccatga aaaggacaac cgcttccgac ccgaggtcgc gcgcattctt cgggatgggg
    12121 cggccttgcc gctcgactga aggtctagcc gggctccttc tggtcgacta agagttgagg
    12181 cgttcccttc accagcaatc ttgtcaccag caaagaagca cgtcgcgcat gcggtttttc
    12241 aagcgtcgcc ccgtcgttcc ggtcattcgc ttttcaggac cgattggatt tgccaccccg
    12301 ctcaggcccg ggctttcgat cggggcagtc gccagcgggc tcgagaaggc gttcagcctg
    12361 tcgaagctgc cgtgcgtggc cgtgaccgtg aattctcccg gcggctcgcc ggttcagtcg
    12421 aacctcatct tcaagcgagt ccgccagctg gcggaggaaa agaagaagaa agtctacgtc
    12481 ttctgcgagg acgtggcggc ctccggcggg tactatctgg ctgtggctgg cgacgaaatc
    12541 tacgccgacc cctcgtccat tgtcggctcc atcggcgtga tctcggcgag cttcggcttt
    12601 aacaaggcga tcgaacggct ggggatcgag cgccgtgtct ataccgcggg cccggcgaag
    12661 ggcctgctcg accccttcca acccgagaag cccgatgagg tcgaacggct gaaggcgatc
    12721 cagagggacg tgcacgacgt cttcatcggc gtcgtcaagg atcggcgcgg cgcgcgtctc
    12781 cgcggccacg atgcggagct cttctcgggg gccttctggt cggcggccaa ggcgctggag
    12841 ctcgggctca tcgacggcgt ctccgatctg cggaccaaga tgcgggagtt gcacggggat
    12901 gacgtgcaac tcaaggtcgt accgttcggt ccgggcggtt ggctgtcgcg cctgctgcgg
    12961 tttccgcggc tcgcgccggc cggcatggac tccctggcca acggcttgcc actttcgctc
    13021 ggcgatgatc tcctgtccgc tatcgagacc cgctcgctat ggtcgcgcta cgggctgtaa
    13081 ctcttgccgg tcatacgtaa accgttcggc gagcttggag ggggacgatg ccgcaggtca
    13141 tcgcgctgct tcttgccgga gccggtctct atgccggttg ccgctgggtg agccgcgagg
    13201 ttcgtcgggc gctggcaacc gcgcaagagg ccgaggaaag attgcgccag caaagcgcgc
    13261 cgaaagacct tggctcgctc gaatgggatg cggaggcggg tgtctaccgg ccgtcgaagc
    13321 aggcctgagg gcaggcgcgg agccgcgcaa caaattcctc gcgatggcga cagtgaaccg
    13381 tctcgccatt ggcgacccgc tcccctatag tccgcgccga tcccgacctt caggcgcaag
    13441 tcattatggc gacacgaacc gcaatcagac cggcaaccga gcccctcggg caaacggtat
    13501 ccgggctgca gccgacgcga tccttccagg acctgatcct cacgcttcag cagtactgga
    13561 gtgagcaggg ttgcgccatc ctgcagcctt acgacatgga agtcggcgcg gggacgttcc
    13621 atccggccac gaccttgcgt gcgctcgggc ccaagtcctg gaacgtagcc tacgtgcagc
    13681 cctcgcgccg gccgaaagac ggccgctacg gcgagaaccc gaaccgcctg cagcactatt
    13741 accagttcca ggtgatcatg aagccgtcgc cgccggacat catcgacctc tatctggaaa
    13801 gcctcgcgcg tatcggcctg tcccgcgaaa tccacgacat ccgcttcgtc gaggacgact
    13861 gggagagccc gacgctgggc gcctggggcc tcggctggga ggtctggtgc gatggcatgg
    13921 aggtgacgca gttcacctat ttccagcagg tcggcggctt cgactgcaat cccgtatcgg
    13981 tcgagatcac ctacggcctg gaacggttgg cgatgtacgt gcagggtgtc gaccgcgtgt
    14041 tcgacgtcag cttcaacggc cgcgaggacg agcggaagct cacttacggc gatgtcttcc
    14101 tccaggcgga gcaggagtat tcccgctaca acttcgagta cgccgacacc gacatgctct
    14161 tccagcactt ccgcgatgct gagaaggaat gcctggcgtt gctggagaag ggtgagagcg
    14221 aggacggccg ccacctgttg gctctgccgg cctacgacca gtgcatcaag gcctcgcaca
    14281 tcttcaacct gctcgacgcc cgtggcgtga tatcggtgac ggagcggcag agctacatcc
    14341 tgcgggtgcg cgagctcgcg aaaggctgct gcgcggcttg gctgaagacc gagggagggg
    14401 gcgcatgagc aggccgaaga cactgctgga aatggccggg gccgatctca gccctggtgc
    14461 gctggcgcag tcggtcgtca tcatcatcga tgcgcagaat gaatatcttg acggcaagct
    14521 cgcgctgccg gggatcaagc cggcgctcga tgcgatctcg accctcctcg ccaaggcgcg
    14581 cgatgccggc acgccggtca ttcatatcgc gcacaagggc cggccgggcg gattgttcga
    14641 tcgcgagggg cacgggggcg ccatcatcgc agccgcggcg ccggcgacgg gtgagccggt
    14701 catcgagaag ccgctgccca acgcattcgc gcaaacggcg ctcgattccg agttgaaggc
    14761 gctgggacgc aagtctctga tcatggccgg cttcatgacc cacatgtgcg tcagctccac
    14821 ggcgcgcgcc gccctcgacc tcggctaccg ggtgacgatc gccggtgatg ctgccgcaac
    14881 acgggatctg cccgacccgt tcggcggtgt cgttccagcc gatgccttgc atcgggcagc
    14941 gctcgccgag ctgggggatc gcttcgcaat catcgcgaca gtcgatcaga tcccaaaaga
    15001 ttgagctgcg tgcttagcgg ggaacgatgg tgatattgcc gtcccgctcg aggacggcaa
    15061 acttgatttg atcgatgcgc tccagccccc gccagcggcg tgcggcctcg agaatatcgt
    15121 caaggtcgac gcgctccagg tccaggcgct cctgcagcgg ccgcccgttt tcgacgagca
    15181 cgaccggctc gccctccatg agcctgtcga cgcccgggta gcgctgcttc aggagggtga
    15241 gcacgacctc gatgaggaac agcgtcgaga tgagcaggat cgcggtgatg atcgagaaat
    15301 catcgccgat gagcgcctgc tgcgtcgttt ccgcgatgat gagcagcagg atgaagtcga
    15361 aagtggttat gtcggcgagg gatcgcttgc ccgccaccct gaacaccagg aacaagaacg
    15421 catagatcac tgcggctctg atgactgtat ccattgtttg cggtcctcac ggccagacga
    15481 attgggtgaa ctgcagccgc gcgccgcctg cgatcagctc ggcatcgagc atgcccgcct
    15541 ttatcggctc tgcgtggagg tcgacgacga gcaggtcaga agcgggatcg gccgcgaaca
    15601 ggaatttggt gagctccgcc ccgctcacga cctgtatggg ctccggcacg atggataaga
    15661 cccgcagcga tcccaaatag tcacgcggca gtgacaactc tgctcgtgtg cttcccggtg
    15721 gaacgggaat cgttgccttt agcgtgaatg gtgttccacg ccggatgaag cgatcgtatt
    15781 cgagcgtcag ctcgtttgcc gcgcgcgtcg tcgaacttag cgggccgctt ccgaacaggc
    15841 cgacggcgcc cgccaggacg agcaatccta tcacggccca gccgaggtgc tgcaggcgcc
    15901 acttccagcg catccgcgcc atttggctgg ctgccgtcag cccgtgcaga tcctcggtcc
    15961 ttgccttgtc catcgctcat tcctgggctg gcaacgccga ggccatgggc cgggttcgaa
    16021 ggagcgattg cgccgcggcc atgcacggag taggccttcg ctccatcacg tgatgccgct
    16081 aaaaggatcg gctcgcgatc ctgccctcga ggaccaccat gcctgaactt ctgctcgagc
    16141 tgttttccga ggaaatcccc gcgcgcatgc agccccgcgc tgccgaggag ctgaagcggc
    16201 tcgtccttgg cgcgctgaaa gaccagggcc tcgccgtggg tgaagctcag gcctttgcca
    16261 cgccccgccg cctgacgttg atcgcgcgcg atgtgccggc acgttcgccc gaccagtccg
    16321 aggagcgcaa aggtccgcgg gtgaacgcgc cggagcaggc catccagggc tttctcaaag
    16381 cggcaggcct gtcctccatc ggcgaggcga gcgtcgtctc agatcccaag aagggcgact
    16441 actacgtggc gcggatcgaa cggccgggcc gcagcgccta cgaggtgatt gcggaggccg
    16501 tgcccgatgt cctccatcgt ttcccctggc ccaaatccat gcgctggggc cgatacagct
    16561 tctcttgggt tcggccgctg catggggtcg tctgcttgct cgatggggag atcgtgcctt
    16621 tcgaggtcgc cggcgtggcg agcgggaaca cgacacgcgg ccaccgcttc cacggcaacg
    16681 tgccgttccg ggtacgtggg atcgacgact atctccagaa gctgagcgcc aaccgggtga
    16741 tcctcgatgc gaccgagcgc atgcaacgca tcgaacatca ggccaaggag cttgcagcga
    16801 agcacaagct gcaactcgtg ccagaccacg ggctgctcgc cgagaatgcc ggcctgacag
    16861 aatggccggt cgtgctgatg gggcattttg acgaggcgtt cctggagttg ccgcccgagg
    16921 tcatcatcac ctcgcttcgc acccaccaga agtgcttcgc gctgcgcgat ccgaagacgg
    16981 gcaatctcgc caaccggttt cttctggtcg ccaacctgga ggccagcgac ggcggcgcaa
    17041 cgatcgtcgc gggcaacgag aaggtcatcc gggcgagatt gtccgatgcc cgctttttct
    17101 gggagcagga cctgaagcga ccgctcgacg agatggcaca tgcgctgcgt ggcgtcacct
    17161 tccacgaaaa gctcggcacg cagtgggacc gcgtggaacg gatcgcggag ctcagctacc
    17221 agatcgccgg ggccgtcgac gccgcacctg aggccgcccg acgcgccgcc cagctagcga
    17281 aggctgacct ggtgtccggc atggtgggcg agttcccgga attgcaaggt ttgatgggcc
    17341 gctactacgc cgaggctgcc ggcgctaagc ccgagatcgc ccgcgcgatc gagctgcact
    17401 acaagcccaa ggggccgaac gacaccgtgc cgtccgcgga ggacggcgac tgtgtcgcca
    17461 tcgccgtggc tttggctgac aagatcgaca cgctggtcgg attctggcgt atcaacgaga
    17521 aacctacggg atcgggtgat ccctatcagc tgcggcgcgc cgcgctgggc gtcattcgga
    17581 tcattctcga gaacgaccta cgtgtttctt tgatcaagcg gatttcggcg gcgcacgcgg
    17641 agtatgacaa gcaagggcaa cgggccgtca aagggagcga ggaggaaatc acggccgacc
    17701 tccttgattt cttcgccgac cggctgaagg tccacttgcg cgagaagggc gcgcgccacg
    17761 atctcatcga tgccgtgttc gcgctgcccg gccaggacga cctggcgctc atcgtcaagc
    17821 gggtcgaggc tctggggaag ttcctggaaa ccgacgacgg cgccaatctc ctggcgggcg
    17881 tcaagcgcgc ggccagcatc ctcgccatcg aggagaagaa ggacaagacg agctacgacg
    17941 gcgattacga cctcaagctg ctcgaagcac ccgaggagct ttccctggcg gcagcgatcg
    18001 aagccaccaa gcaggacacg cgggcggcga tcgcggtgga gaacttcgag ggcgcgatgc
    18061 gggcgctcgc ggacctgcgc cgtcctgtcg acgccttctt cgacaaggtg acagtcaatg
    18121 tcgaggatca gcggctgagg cagaacaggc tgcgcctgct gtcccagatc cgcgcggcga
    18181 ccatgacggt cgcggatttc tcgaagatcg ccgggtaacc tgtgatgctg cagcacctgt
    18241 tcgtctacgg cacgctgatg tcgacgacag gccatcgcat ggccctgcgc ctcgcccgag
    18301 agggccggct cgtcggcccg gccgcggtgc gtggccgctt gtacgacctc ggggcctatc
    18361 cggccctggt cgaggcggag gcggaagacg gggaggtatt tggagaagtg cacgccctga
    18421 cagatcctgc cgcatccttc cgttggctcg atgcctacga gggcatcgtg ccagggcggg
    18481 actgcgagta cgtgcgcgtg cagcgatccg tgcgcctcga gaccggcgcc gagctgacgg
    18541 cgtggctcta cctctaccgg gcgcccgttg caggcaggcg gctcatagag agtggacgct
    18601 ggatgtcacg gttcgcatcg gcgggaggcg tgcgctgatc cggccttaaa cgcgtgattc
    18661 tgccgcaatc tcccatgtca tctgggtgct tgtcaccggg atgcagctgg agttggcaga
    18721 agaataggaa ccgtcttcac gagttcacca acactagagc atttccgatt tagacagaag
    18781 cacgccgagt caatcgcgcc caaaactccc tctccccggt ggggacaggg tcggggtgag
    18841 gggggtccgc cggtggcagc tgttgcaacc cctcacccgg cccttcgggc cgacctctcc
    18901 cccatgggag aggttaacgg agtgcttgct gttcacgttt ccacctggat cggaaaagct
    18961 ctaaaccgaa agctccgtgc tgccgatgcc gtcgagcgag aacacgaacg tatcgccagc
    19021 ggcaatcggg atcgtcggca ggacgctgcc ggtgatgacg accatgccct ccttgagtgg
    19081 gcggccgcga tcggcggcaa gattggcgat ccacgcaagc gcacccattg gatcgtcggt
    19141 gacgcccgtc ccgcgctcgg cgccgttgac ggtcaagtgt ccgcggatgc catcgagctc
    19201 catgtccgcc ggcacacgct gcggggcacc gaggacgatg ccgccgttcc aggcgttgtc
    19261 tgcaatgagc gacagcgcgc tcgtgctctt gtagtccgcc ccgcgatcct cgatcagctc
    19321 gaaggcgggc atcgcctcgg caacggctgg gcggacatcg gcagcggtgt atgggacgtc
    19381 acggcgcggc agatcagcgc cgaggcgcac ggccagctcg aactccacca tcaggttgat
    19441 gaaatcctcg cgcctgagcc gcgcgggcga ctggtgaatg cgccgctcat agatcatccc
    19501 gccgcaaggg tgattgatac ccatgagctg ctgcatgacc ttcgtcgtgg tcgcgatctt
    19561 gagcccgcgg acggggccgt aaatcgggga ccacagctcg cacagcgcct cctgcgcagc
    19621 gtaggcatcc gcaacggtcg gcggggcgat ctcggcgggc aggttacggt aaggtgcgcg
    19681 cgagcggtgt gcttcggcga tgaacgcggc agccgctttc ggatcgaacg tagtcatccc
    19741 aactccctca atcaggacgt gtctcggcaa aagccggcac cgctttgtcg aaagcatcga
    19801 gccacgcgac caggttcggg tgcccggcac gccacgtgcc gccatggcga aaatcgagat
    19861 agccgagagc gcaggcgagc gtcacgtggg cataatccgg ttttggggcc aactcggaaa
    19921 gcgatcgttc gaaccagtcg agggtcgtat cgaccttgag ttgctggcgc tgcatccagg
    19981 tgtcgaccca cttgtcctcc ggccggtagc gcttctcgta gacgagcagc aacgcggcat
    20041 cgagaatgcc atcggccatg gcggcgagcg tgagcgtacg gaagcgctct ggcccggcag
    20101 ccggaaaaag ccgcgggtcc ggagtcaggc tatccaggta ctcgcagatg acagggctgt
    20161 cgtaaacgtg cgtgccgtcc tcgagaatga ggatcgggat cttggacagc gggttctccc
    20221 ggcgcagctc ctcgttgatc agcggatagg tatcgacgcg ccgcttctcg atccgatccg
    20281 ctacgccttt catcgccgcg acgatggaca ccttgcgccc gtaaggggag gccggagagg
    20341 acagcagctt catcataggc ggaatcctgg aactgaaacg cgagggttga gcgcacgacg
    20401 gtgcagcatc gtgccgggtc ggctgacgga tcgcaagcgc agatgcgtat aacgacggcc
    20461 ggttaagcag ctgcaatctc cagcaggccg agcatgatca gccccaccgc aaggccccag
    20521 agcacgagct tgccgcggcg ggtctggctg ccgcgctcca tgaccagcag cgttatgatc
    20581 acggccgcgc cgatcaggtt gatcagcacg agcccgccga ggccgagccc tttcccgccc
    20641 gcgatgctgc cttcgagcag aatggcggcc gccatgccga tcagcagtgc accgagcagc
    20701 cgcggccaga gcaacgcctg cggcaacggc aagcccagaa tgcgtgctgt tgtcagtggc
    20761 atgatcagca gcacgagccc ggatgagaac cggagcagcg tctcgagcca gagcagctgg
    20821 tcggccatgc aacggccgct cccgtcgcgt cgcgggcgcc tcagcgttgg gcaggcgatc
    20881 ggcccagatc gtcgaagaat tcgtcaggct tccacttctt cttgggggcg tcgtcgatga
    20941 gcccggcgaa cagcccctgc tgcggctcct ccttcttggg tctcggcgtg aagtcgcctg
    21001 cgagatggcg attgagatcc tccttgatct ccttggtctt gagcttgtcg caatagtcgg
    21061 cttcgtcggc gatcttcttc gcgacggaca ggcgaacgta ctcgtactcg cgctcgatct
    21121 tctgcaactg ctcggggcca gcgccttgcg ccatctcggc ggccaggaag ttcgccttga
    21181 gcttctccgg gtcgaaatag aagccgcagc gggcggcgcg agcggacgtg gcgcccacct
    21241 gcacggcgcg cgaggtcgga tcgctcggcg gcggtccggc cggagccgat gacgcggctg
    21301 ccgcttgtgc agtctcttgc ccgcctccac ccgtcagcgc tgccgtggtg aggcttcccg
    21361 agccgctaca gccggcgagc agcagggcgg tggcggcgag catcgaaaac ggcatgcgat
    21421 tcggcacggg tgcccccctt ttgctgcatc ctccctgtgg aggactgaag gtcccagaac
    21481 attccgtgga cagtgctccc cctccattgg agcgtcaata gcttgaaggc cacgacttgg
    21541 tggctttctg ttgaggatcg acctctaatg ggcactctcg ccggcagctt tctcccgctg
    21601 ccgggcccgg cgggccatca tgttcagagc ttcgacaagg gcggagaacg ccatggcggc
    21661 gtagacgtag cccttcggca cgtgaacgcc gaagccgtcg gcgatcagca ccatgccgat
    21721 catcagcagg aagctcagcg ccagcatgac gatcgtcggg ttggcgttga tgaaattcgc
    21781 cagcggcgta gccgcgagca gcatcaccat gaccgcgacg atcaccgcca ctaccattat
    21841 gggaacgtgc ggcgtcatgc ccacagccgt gatgatgctg tcgatcgaga acacgatgtc
    21901 gagaagcaaa atctgcacga tggccgaccc ggtgccgagc gcaaccgtgc gccggtcgaa
    21961 gatgtcgggt gcggggtcgg ggtcgacccg gtgatggatt tctttcgtcg ccttccagac
    22021 caggaacagg ccgcccgcaa tgaggatcat atcgcgccag gagaagtcga gcccgaagat
    22081 cgaaacgacc ggcgtcgtga gccgaatgat ggcggcgatc gtgaacagca gggccagccg
    22141 gagcacgagt gctgccgaga tgccgatctt gcgcgccatg tcccgccggt tctcgggcag
    22201 cttgttcgtg aggatggcga tgaagatgag attgtcgatg ccgagcacca cctccatcgc
    22261 gatgagcgtg atgagcgcaa cccaggcagc cgggtcggtg aggaggggga ggatggtgtc
    22321 catgggccgg aatgatcctg ctgtggcggt attcaattca cgccgagcga cgtggtggag
    22381 caggtaagcg cgatcaggag ggagcgcaac cggaacaggg gatgaatttc atgatcggca
    22441 gcgatctgcg ggagagcttg cctcggcgct gcaccacgct tcttccggcg cctgcaaccc
    22501 atcacaatgc aaacctgcct tttgccgcaa tgtgccggct aaatgcgact cgggacgaac
    22561 tgcgacagca cgtgccgaat cggcgatgct gtgcatcggt cgacgtgctg aggaaggttt
    22621 cggggggatg aagtcgagca atctttggct cgtggcgtct tgcgtcgtgg tcgccggtgc
    22681 cagcgcggcg gctgtgcttg cgctcgatca cgccgggatc atcagcctta caccgtccgc
    22741 tgagcaaccc gccgagcagg ttccggtgag actggttgaa accgttcctg tggcgcccgc
    22801 tcgccagacg atccccgtcg agcccgctcc cgaaccggaa gagcgattgg cctacttgca
    22861 acccgagaca gcggtgctcc cgccgccgcc cgcgcccgag gaaaagtccg aggcggacga
    22921 gctgccaagc agcgagccgg cggagcccga ggttccggac tcctggagtg cggaagttca
    22981 gcccgaagag acggaaatct ctgacgaagc gcagcttctg gacggacctt cccctgcctc
    23041 gccgccgcgg ttttcgaagc gccgcctgac atcgcacagc gctgccctga caaagcgtct
    23101 ggcggagatc tcccctggag caaacgcgcg gcttgcaaag aaattcgcgg cggccgaggc
    23161 accctggccg ccgcacgaga tcgccctcgt cgcgatcaag gacgagaagt cgctcgagct
    23221 ctttgcgcgc ccggaggacg gcgagtggaa attcatccac cgctatccgg tgctcgcggc
    23281 cagcggcggg cccggtccca agttgagaca gggtgacagg caggtccccg agggcatcta
    23341 caaaatttca ttcctcaatc cgaacagccg ctaccacgtc tccctgcgcg tcaactatcc
    23401 gaacgccttc gaccgtcaga tggcggaagc ggaagggcgc actcggctcg gcggggacat
    23461 catgatccac ggcaaggccg tatctgccgg ctgtctggct gtcggcgatc ccgccgccga
    23521 ggagctcttc gtcctggcgg cccaggtcgg gctcgagaac atcaggctca tcattgcacc
    23581 gaccgacttc cggcggcgcg gattgccggc gatcgaaccc ggacagccga attggctgcc
    23641 gaaactctac accgaggtcg cagccgccat gtcggagttc aagtcgccgc cgcgcatcgg
    23701 cctgttgtcg ttcttcggca actaaggtcg ccgccagccc ggaacgtttt cgcgcgatgg
    23761 ttcgttgccg ccgagctggc gttggccgcg aagcgcgctg gaaggcgttt ccatgctcac
    23821 ccgacggacg ggcgacggtc ctctcctcgt cgaccttgcg ctgcagggcg gcggcgcgca
    23881 tggcgcgttt acttggggag ttctggatcg cctcctggaa gagccggata tccagatcga
    23941 tggtatttca ggaacatccg ccggagccat gaatgcggcc gtcctggcag acggctacgc
    24001 cgctggatgg cgcgagggtg cgcgcacggc actcgaagaa ttctggaacc aagtttcttg
    24061 cgcggccatg ctcagcccgc tccggcgcag cccgatggat atcctgatgg gccgctggac
    24121 gatggacacg tcgcccgcgt tcatcactat ggatctcatg gcgaggctgt tctctcccta
    24181 tgatctcaat ccccacggcg ccaatccgct ccgcgagatc ctgacccggt gcattgatcc
    24241 agagcacttg gtgaacgggc cgatccggct gttcgtcacc gccacgaacg tctcgacggg
    24301 ccgcggccgt gtatttcgca atgccgagct gacgccggat gtcctgctcg cctcggcctg
    24361 cctgccgacg ttgttccagg cggtcgaaat cgacggtgag gcttactggg acggcggctt
    24421 ttccggtaat cccaccatta caccgttggt tcgcgagtgc gccgcgagcg atacggtaat
    24481 cgtgcaggtc aacccgatcg agcgcagcgg cacaccgcgc tcggcgcgcg agatcatcaa
    24541 ccgcgtcaac gaggtctcct tcaatgccac gctgatcaag gagctgcgga tgatcgcgct
    24601 gctgcggcag gtggccgatc ccggcgattg cgagggaaag ctgtgggccg agatgcgcat
    24661 tcaccgaatc gccagcccgg cgatggcgga gctcggctac tcctcaaagc tgaatgccga
    24721 gtgggacttc ctccttgctc tgcgtgatga ggggcgccgc tccgctgacg catttctcaa
    24781 gacccacggc gccgctctgg gaaaacgctc gacgctggac cttgatccgc tgctcgagca
    24841 ggtgtgagcc ggcatgggac tcctcggcat cctggttggg ctcatctttc tcgtctggct
    24901 cgcctatcgc ggctggagcg tcctgctgct ggcacctgct gcgagcatga tcgcggccgt
    24961 tgccgcgggc gagccgctgc tcgcacactg gacgcagacg ttcatgggag ccgccgcgca
    25021 atttctggct caatattttc ctctgtttct gctcggcgca cttcttggca agctgatgga
    25081 cgacagcggc tccattgcag ccatcgcccg ctacctgcag gagcgcttcg ggccggagcg
    25141 cgcggtgctc gccgtcgtca tcgcaggcgc ggtcgtgacc tatggcgggg tgagcctgtt
    25201 cgtagcgttc ttcgtgcttg tgccgatggc catcgcgctg ttccgcgccg gcgaccttcc
    25261 tcaccgtctg atcccggcgg cgatcattct cggaacggcg acgttcacca tgtcggcgct
    25321 gcctggaaca cccgcgatcc agaatgcgat ccccatgccg ttcttcggga cgacgccgtt
    25381 tgcggcgccg gggctcggac tgatcgcatc ggcgatcatg ctgctctttg gactctggtg
    25441 gctcggacta aaggttgcgg cggcgcgcag ggccggggag ggctacgacg ccccggtcga
    25501 ggagattgcg aaaggagcgg ccgacgacga gttcatccga gagcgcgcta cggtcgcccg
    25561 cgagttcgat ccggcggaga tccatcacgg ctgccacagc gcaagtgagc cgcccgtggc
    25621 cgtggcggtt gcgccgcttg tggttgtcat cgcggtcaac gtcctgatgt ccttcgtcgt
    25681 gctgccgcgg ctcgatcttg cgttcctggc cgaggagagg tggggcgcga cgtcgccggc
    25741 cggcgtcgcc ggcgtctggt ctgtcatcac ggcgctaaca gctgccgtcg cgacggtcat
    25801 cctgctaaac cgccagcgac tgccgggtct acgcgagacc atggatgccg gtgcgaacgc
    25861 atcggtgctc ccggccctca gcgtggcaag cctggtcggc ttcggcgccg tcgtcgccgc
    25921 attgccggcc ttcgatgccg tgcgtgactg ggtgcttgcg atcgagggtg gccccctcgt
    25981 gtccctggcc gtttccacca acatcctggc gtcgctgacc ggctccgcga gcggcggcct
    26041 ttcgatcgcg ctcggtgcgc tgggcgagaa cttcatgcgg cttgccgctg attacggcat
    26101 cgaacctgcc ctcatgcatc gggtctccgt aatcggtgcg ggaacgctcg acgtgctgcc
    26161 gcacaatggc gccgtcgtca gcctgctggc cctgtgcggc gcgacgcacc ggacaagcta
    26221 tctggatctc gccatcgtcg ccgtcggagg ggccctgtta gggctgatcg cgattattgt
    26281 gctgggctcg cgttttgggg ctttctgaat cgatatgacg cgcgtttatc aactgaagag
    26341 ggagccggat gacaggcgtt tccggcggaa ctaagctgag agatcggggg gtgcagcttc
    26401 gaaggcatga ggtgcaccat ggcccaacaa gcgacacagg ctgatttcga cgcactcctt
    26461 ggtgaaaccc ggcgagatgt tgcgaggcac tggggctggt tcctagcgct cggcattctc
    26521 cttgtcatcg tcgggatggt ggcgatcgcg tttcccttcc tctcgaccat agccgcgaaa
    26581 atcgccctcg gctggatttt gctgatcgcc ggcatcggcg cggtcattca ttccttctac
    26641 gtcaagcggt ggggaggatt tctctggaat ctactcatcg gcctgcttta cctgattgta
    26701 ggcttttggc tcgccttctt tccgctcacc ggaatactga ccctgaccat cgtgctcgcc
    26761 gcgctgttca tcgccgacgg catcatggag atcatcatgg gcttccgggt gcggcctcac
    26821 gaaggttggg gatggttgat cttcagcggc gtgattgcca tcctcgctgg cctcttgatc
    26881 gccctgaacc tgccgagctc ggccgtctgg gctttggggc tgcttgccgg ggtgaagctg
    26941 ctggtctcgg gctggagctt cattttcctg gcactcaccg gcaagagcat gagcgagcag
    27001 acagcgacga cggcaacctg acaatacgag ccattgatgg ctcgtagcta ctcaggtggg
    27061 ctccgtttcg ctcggcccga cgatcgtcag cggcggggag ccgcgtgagt tcgcttcgac
    27121 tgctgctgaa gccgcttggc tggctcggcg ccggtttggc aattctcatt cgggtgctcc
    27181 tcgtcgcgtg ggctaccctc gcgatctatt attcgaacct gccgtggcca gcgctgcggg
    27241 tggtcctcgc catcgcgttt gcggcgtttg cgacgtgggc catctggttt tcgcgtcagc
    27301 ggcgcgcgca tggggcgctg gccgttcttt atcttggcgt gatcctgtgg tggcttgcca
    27361 ttccgccgtc gcacgatcgg cattggcggc cggaagttgc cgtcatgccg cgcgcaattg
    27421 tcgatggcga ccgcgttcat ctcacgaatg tgcggaattt cgtttttcgg acggccgatg
    27481 atttcacggt gcgttacgag gagcgcgacg tgcgtctgtc gatgctgacg ggcatcgatt
    27541 tctacatttc attctgggcg aaaggccccg tagggcacac gttcctaagc ttcacctttg
    27601 ccgatgcgcc accgctcagc atttcgatcg aaacgcggcc tgaggtgggg gaggatttcc
    27661 atccactcgc ctccctcttc aagcagttcg agctaatcta cgtcgtcggc gacgagcggg
    27721 acatcgtggg cttgcgcacc aatcaccgcg acgaaagcgt gtttctctac cggctcaata
    27781 catcgcccga aaatgcccgc cggcttttcc tggtctacct cgatcggatc aacgagctcg
    27841 ccgatcagcc cgaatggtac catctcttca gcaacaactg cacgctgaac attattcgat
    27901 atgccaacgc cgccgggcgg cgcggccggt tcgacatacg gcacctgctc aatggcttga
    27961 tcgacagcta cctctattac tcgggccgat tggacacgtc ccttccgctc gacgagctgc
    28021 ggcggctctc gcggatcgac gaagcggccc aggtagccga taactcccct gatttttcag
    28081 agcgcattcg ggctgcgctg ccgtcggtga cccgccgcta atcgatgcag cagggcaggc
    28141 ggggatctgc cctgctgcgc agccgtttac aacctcgctt ctaaaatcag gttgaacggc
    28201 gtctccacgg ccttgcggat cgtcttgagg ccggcctcct tgagcacgtc ctcgaggcgc
    28261 cgctgaccgg cctgcgcgcc cagcacgtgc gtgccgttct cggagatggc gtgggcacag
    28321 cacatggtgg tcgaggcggc gtaatagagg cggccgatgg ggttgatgtt ctcctcgaca
    28381 cggtcgcggg cgaatggctc gatcagcatg agcgtgccgt ctttggccac ggcttttatc
    28441 gcgtgccgca cggcatcgac cggccgaccc atatcgtgca ggcagtcgaa gaagcagatc
    28501 agatcgtagc cgcgagcggg gtatgtgtcg gccttcgcca cctcgaaggt cacacggtcc
    28561 ccaacgcc
//
LOCUS       DASVDU010000300        10377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_305776, whole genome shotgun sequence.
ACCESSION   DASVDU010000300 DASVDU010000000
VERSION     DASVDU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10377
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305776"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..397)
                     /locus_tag="VF226_20190"
     CDS             complement(80..397)
                     /locus_tag="VF226_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7076364.1"
                     /translation="MAGKREAELNAPPHPGEYLREDILPALGMTIADLARHLGVTRTS
                     LSQLVNEKRDVSLEMAQRLGQAFGNGARFWLALQMQHDAWKAERNSKIRVQRIRWQGS
                     KAA"
     gene            complement(387..677)
                     /locus_tag="VF226_20195"
     CDS             complement(387..677)
                     /locus_tag="VF226_20195"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7076365.1"
                     /translation="MLDPKRITNRALRRYWEAGDGSKINAQWRRKVWRVLNALDVAAA
                     PQELDIPGFGFHELKGNRAGTYAVSVSGNWRMTFRWSNEGPYEVELEDYHGR"
     gene            complement(734..1663)
                     /locus_tag="VF226_20200"
     CDS             complement(734..1663)
                     /locus_tag="VF226_20200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEX7076366.1"
                     /translation="MAEDPYALLGVPKNASDEDIRRAFRKLAKELHPDLNPGDKAAVE
                     RFKKVSAAYELLGDPEKRKRYDRGEIDASGEPRHAYQRQGAAGAGPFGWRGARPGGPP
                     PDFEDIGFSDIFSEIFGGARGARGTRFTGGIRGSDVRYTLEIDFLEAVTGARKRVTMP
                     EGGMLDLTVPEGVQDGQVLRLKGKGGPGFRGGEPGDALVEIKVRPHPQFKRVGDDITL
                     DLPISIDEAILGAKVEVPTISGRVQLTIPKGTSSGRVFRLRGKGVRNVTAGTTGDQLV
                     TVRIVLPDKIDDSLAYFMSEWRQRHAYNPRERG"
     gene            complement(1760..3286)
                     /locus_tag="VF226_20205"
     CDS             complement(1760..3286)
                     /locus_tag="VF226_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7076367.1"
                     /translation="MEGPKRLGSPPRDGAPGPVGVIDIGSNSVRLVVYEGAMRAPTPI
                     FNEKVLCGLGRSVATTGMLGGEAVERALAALRRFGAIARILDVKQLSAVATAAVREAR
                     DGPAFIARAEQACGCAVQVLSGSREAELAAQGILMGFGAVNGVAGDLGGGSLELIDIA
                     NAHLRQAETLPLGGLRLLDLTRNRMDEALALVEAEIGKLAWLGAEPSRTFYAVGGTWR
                     TLAHLHMKARDYPLHVMQGYAIPTDEAIAFCRDVRSRKEIEGFERIARARREVLPLGA
                     LVMERLLKRLQPEKVVFSVFGIREGLLYDLLPEDERSKDPLLSFCEDFARLRSRSAEH
                     GRELATWTDAIFTEPGPAETPEERRLRHAACLISDVGWRAHPDYRGEQSLNLIAHSAL
                     TAIDHPGRLFLAMAIYFRHVSGGGGNSDELSSRIKRMMDKRALRRARVLGAAIRTAHM
                     LSVGRPGIIVQTPLSYERGRLVLSIPPTYADLDGERLRRRFAALADLLDMPGEVRLTT
                     "
     gene            complement(3405..5582)
                     /locus_tag="VF226_20210"
     CDS             complement(3405..5582)
                     /locus_tag="VF226_20210"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEX7076368.1"
                     /translation="MSAVEQAADIRREPSEPPKGPARFYNRELSWLQFNRRVLEEALN
                     RRHPLLERLRFLSISASNLDEFYMVRVAGLYGQVTAGVATISADGLTASQQIEEINRF
                     ASSLVADQQACWAALRDELSEAGIRLVEAKDLKPAERSWLDGQFMERLFPILTPMNVD
                     PAHPFPFIQNRALTLAVKMLHPEGKVMKGLIPIPAQLDRFIRLPTDPEDQRIIRFMRL
                     ETVIGLYLEELYPTFTITAQGAFRVLRDSDVEFQEEAEDLTRAYESQLKRRRRGDVIR
                     LEVDAEMPEDLRQFVTDELEVADAAMLLNEGLLGLADTSQLIVRDRPDLLFKPFNIRF
                     PERIREFSGDVFAAIRAKDFVVHHPYESFDAVLQYLRQAVADPNVMAIKWTLYRTLRE
                     KSPIVEALKEAADAGKSVTAVVELKARFDETTNISLARDMEKAGVHVVYGFVELKTHA
                     KLGLVVRREGNDIATYCHIGTGNYHPQTARVYTDLSFFTADKAIGRDVARIFNFVTGY
                     GKPQDLEVMAASPHGIRARILEHIREEIRHAKAGRPAAIWAKMNALVDPQIIASLYEA
                     SAAGVEIDLVVRGICCLRPGIPGLSENIRVKSLIGRFLEHSRIYCFGRGEGLPSPNAA
                     VYISSADMMPRNLDRRVEAMVPITNPTVHEQVLDQIMLANLKDNQQSWQVLADGSSVR
                     IKPAPGEEPFNAHQYFMTNPSLSGRGQALKENMPPRVRLTLRD"
     gene            complement(5656..6348)
                     /locus_tag="VF226_20215"
     CDS             complement(5656..6348)
                     /locus_tag="VF226_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX7076369.1"
                     /translation="MSDIPEQLTLELPHRAAYGAEDFLVSGCNEAAVAGIDRWPDWLH
                     PAMAVVGPAGSGKSHLVNVWRTRSGAVVLRAAELDEASAARFEAHRAIAIEDVDRGVA
                     DERVLFHILNMAAEQKKSVLLTSRHALGELAITLPDLRSRLRAVPLLSIANPDEALLR
                     ALLVKLFTDRQLVVEPHVISYLALRIERSTEAVARTVAEVDRAALASRRKVTRALVAE
                     VLARLESDEAGV"
     gene            complement(6345..7448)
                     /locus_tag="VF226_20220"
     CDS             complement(6345..7448)
                     /locus_tag="VF226_20220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7076370.1"
                     /translation="MRVERQVLFWLAALILLILAALVLRSVLLPFVTGMVIAYALNPV
                     ADRLCAIGLPRIWASAIVVTLLMMALVAAIVFLVPILAKQIQQFIEVLPGELERLRKT
                     IEASARERLGSRFTDFQIWLDSAAAELSRNWASVVGILAQSVWAQGRALVSFASLLLI
                     TPVVVFYLLVDWHPMLDKIDGWLPRDHAPTIRRLALEINAAVAAFIRGQGLVCLVLGS
                     LYAVGLTWAEIPYGLVIGLGAGLLSFVPFVGFAVGLITALVVSLVEYWPETLPILKVL
                     AVFGVGQALDSSLLSPRIVGPKIGLHPVWLIFALFVFGYLFGFVGVLVAVPVAAAIAV
                     LVRFALEVYLSSSIYKGRSGDVAVPLEPVEKRP"
     gene            complement(7565..8119)
                     /locus_tag="VF226_20225"
     CDS             complement(7565..8119)
                     /locus_tag="VF226_20225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7076371.1"
                     /translation="MSIPNLITMARAILVPIVFWLLVSGRIQAAFVLFVIAGISDAVD
                     GYLAKRFNWQTQLGAYLDPLADKMLVVSIFIALGVLKELPSWLVIAVVSRDILIVMAV
                     VLSWLLNHPVHIKPLIVSKINTVTQLVLAAAVLADAGFGLGIERIRVALVWATAASTL
                     ASLIAYMRAWLAHMAGYETGRPGA"
     gene            complement(8234..9382)
                     /locus_tag="VF226_20230"
     CDS             complement(8234..9382)
                     /locus_tag="VF226_20230"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX7076372.1"
                     /translation="MSLLGKHGVGGFIAGLLAAVALGTATAVPVAADEPAYTVAKYPV
                     DAVAENAVAAKQKALADGQKAAFRSLMKRLVPVTSYDQLDRVMDAKVADLIDSVAVRQ
                     EQTSATQYIASLDFSFRPDAVRNLLERERMPFTDEQAPKVTVVPIYAAPSPGQGPVPA
                     ALSLPRGTAMWREVWAGLDLEHALTPVVIGEMKPELTSEVIQKLINGDGTALRNLYKA
                     YDSSLVLLAVAQPDLAKERLNVSLTGRDAVGAFVLNRSYRMSLDDAAYTSELAAVVSL
                     GIIEGRWKETKVSRGTGPVAGYGAGASTQVQLLVEFRSMRQWQEMRDWIARLPGIEGI
                     QVGGLTARSADIVADYPGGPEGLARALAAQGLDVRQIGNDTLQVRPAL"
     gene            9562..>10377
                     /gene="purM"
                     /locus_tag="VF226_20235"
     CDS             9562..>10377
                     /gene="purM"
                     /locus_tag="VF226_20235"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7076373.1"
                     /translation="MTEPGQPPKAPVTYRDAGVDIDAGNALVDAIKPLAKATRRLGAD
                     ADLGGFGALFDLKRTGFVDPLLVASTDGVGTKLKIAIDTGRHETIGIDLVAMCVNDLL
                     AQGAEPLFFLDYFACGKLDVAAARTVIAGIAEGCRQAGCALIGGETAEMPGMYAAKDY
                     DLAGFAVGAVERGALLPRTDIAAGDVLIGLASSGPHSNGYSLIRRLVASEGLNWDAPA
                     PFAPSRSTAEAMLVPTRIYVKPVLSAIRATGAIKAVAHITGGGLVENLPRVLPA"
BASE COUNT         1905 a         3447 c         3271 g         1754 t
ORIGIN      
        1 cccggccgct gttgtgtgac gggttgcgaa gtgccggagg tgccgaaggc agcagagtga
       61 gccgcgcaag ctgtcggctt cacgccgcct tcgagccttg ccaacgaatg cgttgcactc
      121 ggatcttgct gtttcgttcc gccttccaag cgtcgtgctg catctgcagc gccagccaga
      181 accgggcgcc gttgccgaag gcctgcccca gacgctgagc catttccaga gaaacatccc
      241 gcttttcgtt cacaagctgg gacaggctgg tgcgtgttac gcccaagtgc ctggccaagt
      301 cggcaatggt catgcccaga gctgggagga tgtcctcgcg caggtactct cccggatggg
      361 gtggcgcgtt cagttcggcc tcgcgcttac cggccatgat aatcctccag ttctacctcg
      421 taaggtcctt cgttgctcca ccggaacgtc atccgccaat tacctgagac gctgacagcg
      481 taggtcccgg cccgattccc cttgagttcg tggaaaccga agcccgggat atcgagctct
      541 tgcggcgcgg cggcaacatc aagcgcgttc agcacacgcc agaccttgcg tcgccactgc
      601 gcgttgattt tgctgccgtc gcccgcctcc caataccgcc gcagcgcgcg gttcgtgatc
      661 ctcttcgggt cgagcatgga tgcgttacgt gccacgtaac gcgcaacgcg tcaatgagtg
      721 cattgacctc ggatcacccc cgctcccgag ggttatacgc gtggcgctgg cgccattcgg
      781 acatgaagta ggcaaggctg tcgtcgatct tgtccggcag gacaatgcgg acggtgacga
      841 gctgatcgcc cgtcgtgccg gcggtgacgt tccgcacgcc cttgccgcgc agccggaaaa
      901 cccggccgga gctggtgccc ttcggaatgg tgagctggac gcgcccactg atggtcggca
      961 cctcaacctt ggcgccaagt atggcctcgt cgatgctgat cgggagatcc aaggtaatat
     1021 cgtcgccgac acgcttgaac tgcggatgcg gacgcacctt gatctcgacc agggcatctc
     1081 ccggctcgcc gccgcggaag ccggggccac ccttgccttt cagtcgcagc acctgaccat
     1141 cctgcacgcc ctcgggcacc gtcaggtcga gcatgccgcc ttcgggcatg gtcacgcgct
     1201 tgcgggcgcc cgtgacggct tcgaggaagt cgatctctag ggtatagcgg acgtcgctgc
     1261 cgcgaatgcc gcccgtaaag cgtgtgccac gagcaccgcg cgcgccgccg aagatctcgg
     1321 aaaagatgtc ggaaaagccg atatcctcga aatccggcgg cggccctccc ggccgggcac
     1381 cacgccagcc gaacggaccg gcccctgccg cgccctgccg ctgataggcg tgccgcggct
     1441 cacccgaagc atcgatctcc cctctgtcat agcgcttgcg cttctcggga tcgccaagca
     1501 gctcgtaggc tgccgaaacc ttcttgaagc gctcgacggc agccttgtcc ccaggattga
     1561 gatccggatg cagttccttc gccagcttgc gaaacgcgcg gcgtatgtct tcgtcggagg
     1621 cgttctttgg gacgcccaga agcgcatagg gatcttctgc catggcttac aagactagcg
     1681 ctgtctcatg tcatctcatg agagcttaac atcatttgca gggactggcg cagtgcgcct
     1741 tctgctgtgc accgtcgcct cacgttgtca accgtacttc accaggcatg tcgagcagat
     1801 cggcaagtgc cgcaaagcga cgccgcaggc gctcaccgtc gaggtccgca taggtcggcg
     1861 gaatgctgag aaccaggcgg ccccgctcgt acgagagcgg cgtttggacg atgatgccgg
     1921 gcctgccgac ggagagcatg tgcgccgtcc ggatcgctgc acccagtacg cgagcgcgcc
     1981 gcagggcgcg cttgtccatc atgcgcttga tgcgggaaga cagctcatcc gagttgcccc
     2041 cgccgccact gacgtgccgg aaataaatcg ccatggcgag gaacaggcgg ccgggatgat
     2101 cgatcgcggt aagcgccgag tgcgcaatca agttgaggct ctgctcaccg cgataatccg
     2161 ggtgcgcacg ccagccgaca tcggagatga ggcaagcggc atgccgcagc cggcgctcct
     2221 cgggggtttc ggctggcccc ggctcggtga agatcgcatc cgtccaggtg gcaagctccc
     2281 ggccatgctc tgcggagcgc gaccgcagcc gcgcaaagtc ctcgcaaaag ctgagcagcg
     2341 ggtccttgct ccgctcgtcc tccggaagca ggtcatagag caggccctcg cggattccga
     2401 agacggagaa cacgaccttt tccggctgca aacgcttcaa cagccgctcc atgacgagcg
     2461 cacctagggg aagcacctct cgccgggcac gtgctatcct ttcgaagccc tcgatttcct
     2521 tccggctgcg cacgtccctg cagaaggcga tcgcctcatc tgtcgggatg gcatagccct
     2581 gcatcacgtg cagtggatag tcacgcgcct tcatatgcaa atgggcgagc gtgcgccacg
     2641 tcccgccgac agcatagaac gtgcgggacg gctctgcgcc caaccacgcg agcttgccga
     2701 tttcagcctc cacgagggcc agcgcctcgt ccatcctgtt gcgggtgagg tccagcaggc
     2761 gcaagccgcc gagcggcagc gtctccgcct ggcgaaggtg ggcgttcgca atgtcgatga
     2821 gctccaggct gccgccgccg agatcaccgg caaccccgtt cacggctccg aagcccatca
     2881 ggatgccctg tgccgcaagc tcggcctccc ggcttcccga caggacctgc acggcgcagc
     2941 cacaggcttg ctccgcgcgg gcgatgaagg ccggaccgtc ccgggcctcg cgcacggcgg
     3001 ccgttgcaac tgcgctgagc tgcttcacat cgaggatgcg cgcgattgcc ccaaagcggc
     3061 ggagggccgc caaggcgcgc tccacggcct ccccgccaag cattccggtg gtcgcgaccg
     3121 agcggccgag gccgcacagc accttctcgt tgaagatcgg cgtcggcgcc cgcatcgcac
     3181 cctcgtagac cacgagccgc accgagttgg aaccgatgtc gatcaccccg acgggccccg
     3241 gggcaccgtc gcgcggcgga cttccgaggc gttttggtcc ttccattccg ccgccctgcc
     3301 gaatctaggg tccgaagcgc gaagcttgcc cgggtccggt gcaaagcccg tttatgaagt
     3361 tcgaaaacat gacgggagca agcatcgtga tgctgctcat cgcactaatc cctgagggtg
     3421 agcctcaccc gcggcggcat gttctccttg agagcctggc cgcgacccga caggctcggg
     3481 ttggtcatga aatactggtg tgcgttgaat ggttcctcgc ccggcgccgg cttgatccgc
     3541 accgaggagc cgtcggccag gacctgccag ctctgctggt tgtccttgag attggccagc
     3601 atgatctgat ccaaaacctg ctcgtgcacg gtcggattgg tgatcggcac catggcctcg
     3661 acgcgccggt cgaggttgcg gggcatcatg tcggcggagc tgatgtagac ggcagcattg
     3721 ggggacggca agccttcccc gcggccgaag caatagatgc gcgaatgttc gaggaaacgc
     3781 ccgatcaggc tcttcacgcg gatgttctcc gacaggcccg ggatgccggg cctcaggcag
     3841 cagatgccac gcacgacaag atcgatttca acgcccgcgg cgctcgcttc gtagagagag
     3901 gcgatgatct ggggatcgac gagggcgttc atcttcgccc agatggcggc aggccggccc
     3961 gccttggcgt gccggatctc ctcgcggata tgctcgagga tgcgtgcccg gatgccgtgc
     4021 gggcttgccg ccatgacctc gaggtcctgc ggcttgccgt agccggtgac gaagttgaag
     4081 atccgagcca cgtcacggcc gatcgccttg tcggcggtga agaacgaaag gtcggtgtaa
     4141 acgcgcgccg tctgcgggtg atagttgccc gtgccgatat ggcaatacgt ggcgatgtcg
     4201 ttgccttcgc ggcgcacgac gagccccagc ttggcgtggg ttttgagctc gacgaagccg
     4261 tagacgacgt ggacgcccgc cttttccatg tcgcgggcga gactgatgtt ggtggtctcg
     4321 tcgaagcgcg ctttcaactc cacgacggcg gtgaccgact tgccggcgtc cgccgcctcc
     4381 ttcagggcct cgacgatcgg gctcttctcc ctgagcgtcc ggtagagcgt ccacttgatc
     4441 gccatgacgt tgggatccgc cacggcctgc cgtagatact gcagcacagc gtcgaaggac
     4501 tcgtagggat ggtggaccac gaagtccttc gcccggatgg ccgcaaacac gtcaccgctg
     4561 aactcgcgga tgcgctcagg aaagcggatg ttgaaaggct tgaacaacag gtcggggcgg
     4621 tcgcggacga tcagctggct ggtatcggcc agccccagca gaccttcgtt caagagcatg
     4681 gccgcgtctg cgacctccag ctcgtcggtc acgaactggc ggagatcctc cggcatttcg
     4741 gcatcgacct cgagccggat cacatcgccc cgccgccggc gcttgagctg gctctcatag
     4801 gcgcgcgtca ggtcctcggc ctcttcctgg aactccacgt cgctgtcgcg cagcacgcgg
     4861 aatgcgcctt gcgccgtgat cgtgaaggtc gggtagagtt cctccaggta aagcccgatg
     4921 acggtttcca gccgcatgaa gcggatgatc cgctgatcct caggatcggt gggcagacgg
     4981 atgaaacgat cgagctgcgc cggaatcggg atgaggccct tcatcacctt gccttcgggg
     5041 tgcagcatct tgacggccag cgtcagcgcc cggttctgga tgaagggaaa tgggtgggct
     5101 gggtcgacgt tcatcggcgt caggatcgga aacagccgct ccatgaactg gccgtcgagc
     5161 cacgagcgtt ccgccggctt caggtccttc gcctccacca gccggatacc ggcttctgac
     5221 agctcgtctc gcagcgccgc ccaacacgcc tgctgatccg cgacgagact gctcgcgaag
     5281 cggttgatct cctcgatctg ctgcgaggcc gtcagcccgt ccgcgctgat ggtggcaacg
     5341 ccggcggtga cctgcccgta aaggcctgcc acacgcacca tgtagaattc gtcgagattg
     5401 gacgccgaaa tcgatagaaa gcgcagccgc tcgagcagcg ggtggcggcg gttcagcgcc
     5461 tcctccagca cgcggcggtt gaactgcagc caggagagct cacgattgta gaaccgggcc
     5521 ggccccttcg gcggctcgga aggttctctc ctgatgtcag cggcctgttc caccgcgctc
     5581 atcgatgtct cctcgaccga gatatatggc gtgttcgtta cgatgcagtg atggctggtc
     5641 accaggcaag cgccgtcaca ctccagcttc atccgactcg agccgcgcca gcacctccgc
     5701 caccagggca cgggtcacct tgcggcggct tgcgagcgcc gctctgtcga cctccgccac
     5761 cgtgcgcgca accgcttccg tcgaccgttc gatgcgcagc gcgagatagg agatcacgtg
     5821 cggctccacg acgagctgcc tatcggtgaa cagcttcacc agcagcgccc tcagcagcgc
     5881 ctcgtccgga ttcgcgatgc tgagcagcgg gaccgctctg agccgcgatc gcaggtccgg
     5941 aagcgtgatc gccagctcgc cgagtgcgtg ccggctggtc agcagcacgg acttcttctg
     6001 ctcggcggcc atgttcagaa tatggaaaag cacccgctcg tcggcaacgc ctcggtcaac
     6061 gtcctcgatc gcgatggcgc ggtgcgcctc gaaacgagcc gcacttgcct cgtcgagctc
     6121 cgctgccctg agcacgaccg ccccggatct ggtccgccac acgttgacga gatggctttt
     6181 gcccgacccc gccgggccga ccacggccat tgccggatgc agccagtccg gccagcgatc
     6241 gatgccggcc acggccgcct cgttgcagcc gctcaccagg aagtcctcgg cgccataggc
     6301 ggcccggtgt ggaagctcca gcgtaagctg ctcgggaata tcgctcatgg ccttttctcg
     6361 accggctcca acggcacggc cacgtcccca gatcgaccct tatagatgga gctcgacagg
     6421 tagacctcga gcgcaaaccg caccagcacc gcgatggccg ccgcaaccgg caccgccacc
     6481 agcactccga cgaagccgaa cagataaccg aacacgaaca gcgcgaagat gagccagacc
     6541 gggtgcagcc cgatcttggg cccgacgatc cgcggcgaga ggaggctgga atcgagcgcc
     6601 tgcccgaccc cgaacaccgc caagactttc aggatcggca atgtctccgg ccaatactcg
     6661 accagagaca cgaccagggc ggttatcaga ccgaccgcga atccgacgaa cggtacgaag
     6721 cttaacagac cggcgccgag gccgatgacg aggccgtacg ggatttccgc ccaggtcagc
     6781 cccacggcat agaggctccc caacaccagg cagacgagcc cctgcccgcg aatgaaagct
     6841 gcaaccgcag cgttgatctc caaggcaagg cgtcggatgg tcggagcgtg gtcgcgtggc
     6901 aaccagccgt cgatcttgtc cagcatggga tgccaatcga cgagcagata gaacacgaca
     6961 acgggcgtga tcagcagcag cgaggcgaag ctcaccagcg cccgcccttg cgcccagacg
     7021 gattgagcga gaatgccgac gacgctggcc caattgcggg agagctcggc tgcggcgctg
     7081 tcgagccaga tctggaaatc ggtgaagcgg ctgccgagac gttcgcgcgc tgaggcttcg
     7141 atggtcttgc gcagacgctc cagctcacct ggcagaacct cgatgaactg ctggatctgc
     7201 ttggccagaa tgggaaccag gaaaacgatc gccgcgacca gcgccatcat cagcagcgtg
     7261 acaacgatgg ccgatgccca aattcgcggc agcccgatgg cgcagagccg gtcggcgacc
     7321 gggttgagcg cgtaggcgat caccatgccg gtcacgaatg gcagcagcac cgatcgcagg
     7381 acgagagccg cgaggatcag caggatcagc gccgcgagcc agaagagcac ctgccgttcc
     7441 acacgcatga gatgcttccc tcagcctcaa atgaccccgc ctgacgatgc cgtcggtcgc
     7501 aatatagcgc agttgtggcg cttggtggcc cttgcccacg gcagattaag tcagccagcg
     7561 gagttcatgc gccgggccgg cccgtttcgt agccggccat gtgggccagc caggcacgca
     7621 tgtaggcgat gagcgatgcg agcgtcgagg cggccgtagc ccataccagg gcgacacgga
     7681 tgcgctcgat cccgagacca aacccggcat ctgccaggac ggcagccgcc agcacgagct
     7741 gcgtcaccgt gttgatcttg ctgacgatca acggtttgat gtgcaccggg tgattgagca
     7801 accacgagag caccaccgcc attacgatca ggatgtcgcg cgagacgacc gcgatcacga
     7861 gccaggacgg cagctccttc agcacgccga gagcgatgaa gatggacacc accagcatct
     7921 tgtcggcgag gggatcgaga taggcgccga gctgtgtctg ccagttgaac cgcttcgcga
     7981 gatagccatc gacggcgtca ctgatgccgg caatcacgaa cagcacgaat gccgcctgga
     8041 tccggcccga gacgagcaac cagaagacga taggcacgag aatcgcccgt gccattgtga
     8101 tgagattggg tatgctcaag gtgagatccg accggtctcg gaaatgcttc agttcagttc
     8161 atcgcagccg accgggcacg ctcgctcaaa gccggcgcgc aataatggct gatcgagcac
     8221 atttccagcg atttcacaag gcggggcgta cttgtaaggt gtcgttgccg atctggcgca
     8281 catccagtcc ttgcgccgcc agcgcgcggg ccaagccttc aggaccgccc ggataatctg
     8341 caacgatgtc ggccgagcgg gccgtcaatc cgccgacctg gatcccctcg atgcccggca
     8401 gccgggcgat ccagtcccgc atttcctgcc attggcgcat gctgcgaaac tcgaccagca
     8461 actgcacttg ggtcgaagcc cctgcgccgt atccagcaac gggcccagtc ccgcgactga
     8521 ccttcgtctc cttccagcgc ccttcaatga tacccaggga gacgactgcg gcgagttcac
     8581 tcgtgtaggc cgcatcgtcg agcgacatgc gataggagcg gttgagcacg aacgcaccga
     8641 cggcatcccg ccccgtcaag gacacgttga ggcgctcctt cgccagatcc ggctgtgcca
     8701 cggcgagcag gaccaggctc gaatcatagg ccttatataa attccgcagg gcggtgccgt
     8761 caccgttgat gagcttctgg atgacctcgc tcgtcaattc cggcttcatc tcgccgatga
     8821 cgaccggtgt cagggcgtgc tcgaggtcca gcccagccca gacctctcgc cacatggcgg
     8881 ttccgcgagg cagcgacaat gcggcaggaa ccggaccctg acccggtgat ggcgcggcat
     8941 agatgggaac gactgtgacc ttcggtgcct gctcatccgt gaacggcatg cgctcccgct
     9001 cgagcaggtt gcgcacggca tccggtcgga aggagaagtc gaggctcgca atatactgag
     9061 tggccgacgt ctgctcctgg cgaacggcga cactgtcgat caggtcggcg accttggcgt
     9121 ccatgacgcg atcgagctga tcataggacg tcacgggaac cagccgcttc atgagagagc
     9181 ggaaagcagc cttctggccg tccgccagcg ccttctgctt ggccgccacg gcattttccg
     9241 cgaccgcgtc gaccggatac ttcgccacgg tgtaggccgg ttcgtccgca gcgacaggca
     9301 cggcagtcgc cgttccaagc gccactgccg caagcaggcc agcgatgaag ccgccgacac
     9361 catgctttcc gagcaagctc acgaaccccg ccctcctcaa accagttcac cgatcgcgca
     9421 agttgcccgc agccttggaa gtctgctagg acgcccatct cgcggggagc aatggggcca
     9481 aaatggggtc cggtcaatgc cccggccgct gagttcccgt gcgcggcacg caccccgccg
     9541 gaccatattg cgaaggatac gatgaccgag ccaggccagc ccccgaaggc gcccgtcacc
     9601 tatcgcgacg cgggggtcga catcgacgcc ggcaacgcct tggtcgatgc gatcaagccg
     9661 ctggcaaagg ccacgcgccg gctcggcgcc gatgcggacc tgggcgggtt cggcgcgctg
     9721 ttcgacctga agcgcacggg cttcgtcgac cccctgctgg tagcgagcac cgatggtgtc
     9781 ggcaccaagc tcaagatcgc catcgacacg ggccggcacg agaccatcgg catcgacctc
     9841 gtcgccatgt gcgtgaacga ccttctcgca caaggcgccg agccattgtt ctttctcgat
     9901 tatttcgcgt gcgggaagct cgacgtcgcg gcggcacgca ccgtaatcgc cggcatcgcc
     9961 gagggctgcc ggcaagccgg ctgcgcactg atcggcggcg agaccgccga gatgccggga
    10021 atgtacgcag ccaaggacta cgacctcgcg ggattcgcgg ttggggccgt cgaacggggc
    10081 gcgctgctac cccgaacgga catcgcggct ggggacgtcc tgatcggcct cgcatcgtcc
    10141 ggtccgcact ccaacggata ctccctgatc cggcgcttgg tcgccagcga gggcctgaac
    10201 tgggatgcgc ccgccccgtt tgcgccgagc cggtcgacgg ccgaggcgat gctcgtgccg
    10261 acgcggatct atgtgaagcc cgtgctctca gccattcgcg cgacaggggc catcaaggcc
    10321 gtcgcgcata tcacgggtgg cggtctcgtc gaaaacctgc cgcgcgtgct gccggca
//
LOCUS       DASVDU010000301         9017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1172277, whole genome shotgun sequence.
ACCESSION   DASVDU010000301 DASVDU010000000
VERSION     DASVDU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9017
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172277"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..714
                     /gene="msrB"
                     /locus_tag="VF226_20240"
     CDS             265..714
                     /gene="msrB"
                     /locus_tag="VF226_20240"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006468626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7076374.1"
                     /translation="MPTYRKDPDAIAKLTREQYRVTQENATERPGTGEHLHNKEPGIY
                     VDIVSGEPLFASSDKYESGCGWPSFTKPIEPLNINERRDTSYGMIRTEVRSAHGDSHL
                     GHVFPDGPPDRGGLRYCINSASLRFIHRDDMEAEGYGAYLDQVEDVR"
     gene            complement(735..1307)
                     /locus_tag="VF226_20245"
     CDS             complement(735..1307)
                     /locus_tag="VF226_20245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX7076375.1"
                     /translation="MAQFKVGYLVGSLAKESINRKLAKALVRLAPERLELSEISFKEL
                     PFYSYDYDSDYPEVARQFKKAIESSDAILFVTPEYNRSVPGALKNAIDWASRPYGTNS
                     FANKPSAVIGASIGAIGTALSQRDLRAILSYCNSPQLTAPEAYIHFKPGLITDDGEVT
                     VPSTEEFLRGYMQAFDAFLERVLPALPRKT"
     gene            1551..2651
                     /gene="nirK"
                     /locus_tag="VF226_20250"
     CDS             1551..2651
                     /gene="nirK"
                     /locus_tag="VF226_20250"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEX7076376.1"
                     /translation="MFTRRETLVGVAGAAILAAMPKTPVAAEQIDVARLPRKKVTLLP
                     PPFVHAHDQVAKGGPMIVEFTMTIQEKPVVIDDDGTTMQAMTFNGSIPGPMMVVHEGD
                     YVELTLINPAGNEMPHNIDFHAATGALGGAALTHVNPGEQVTLRFKATRSGTFVYHCA
                     PEGMIPWHVVSGMSGTLMVLPRDGLKDERGRPIRYDTVYYIGENEFYVPRDENGNFKT
                     FESLADSYADTIEAMRGLIPTHVVFNGRVGALTGEGALKAKVGETVLFVHSQANRDTR
                     PHLIGGHGDYVWEEGKFANPPAKDLETWFIRGGSAGAAIYTFRQPGIYAYVNHNLIEA
                     AELGATAHIVVEGEWDDDLMTQVKAPGPITTN"
     gene            2736..3596
                     /locus_tag="VF226_20255"
     CDS             2736..3596
                     /locus_tag="VF226_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX7076377.1"
                     /translation="MKLSVPIAAAAAASVLAAGLILQAPPRRATTDLAIKTITIEPEP
                     FQYRLPGEFLKNGRIADGPKQSTALSRPLEIMAYQVSAAEYAACVADGACRPADGPGT
                     PSDLPVTGVSFHDAEAYAGWLSEKTGERWRLPTDEEWAFAAAERFSDDSLEIVETDAA
                     NPAARWIAAYRERSVGKSRDTTPKPRGTFGANGRGLIDVAGNVWEWTSTCYTRAHIDA
                     DGEIVQTTENCGVRVTGGRHRGYMTSFLRDGKSGGCAAGIAPDNLGFRLVKDPAPTIA
                     QRLLGRFSRS"
     gene            complement(3893..4330)
                     /locus_tag="VF226_20260"
     CDS             complement(3893..4330)
                     /locus_tag="VF226_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HEX7076378.1"
                     /translation="MKRMIAFAALTTMLLAGGAQAAEHEIHMLNKGQKGAMVFEPDLV
                     KAAPGDTIRFVPTDKSHNAEAIKGMIPEGAEGFKSKINEEFTVTLTKEGVYGVKCMPH
                     YGMGMVALIVVGDPVNLDAAKAVSHPGKAKKVFEELFAELPAD"
     gene            4507..5211
                     /locus_tag="VF226_20265"
     CDS             4507..5211
                     /locus_tag="VF226_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7076379.1"
                     /translation="MSRLDRSLIAGLPLFSGMSPADLDKMLATATSRRVPKDGYVFEQ
                     DAEAKSFFVLLNGRVRVVKTTPDGHQVIVRYIGAGELLGIAHAIGRTTYPASAVAVVD
                     CVVLAWPSRLWAEFATAFPSFGANTYKTVGERLQDVQSQVVELATQQVERRVAHALLR
                     LVQQTGKKSDEGLLIDFPISRQDIAEMTGATLHTVSRLLSAWEQQGLVKSGRQRITVV
                     EPHRLLLIAEGRSEKS"
     gene            complement(5223..5423)
                     /locus_tag="VF226_20270"
     CDS             complement(5223..5423)
                     /locus_tag="VF226_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEX7076380.1"
                     /translation="MTGRYRDDMTMDEIMRRWPATIRVVLRHQLLCVGCPIAGFHTVA
                     DAIREHGLDSERFRRELMAAIN"
     gene            5770..6729
                     /locus_tag="VF226_20275"
     CDS             5770..6729
                     /locus_tag="VF226_20275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7076381.1"
                     /translation="MLENSSNRLPQLDGGLFLTDGGIETTLIFHEGLDLPYFAAFHLM
                     KSQEGRAALVRYFEQYVAIARADGTGFIFESPTWRASSDWGALLGYSRNDIAAVNRES
                     IALMRELQAKHGVPQLPMAVSGCVGPRGDGYDPGQVMSADEAESYHAHQIGAFAEAEA
                     DMVTAITMTNTNEAIGITRAAMKAGMPVAISFTTETDGRLPTGQSLADAIAEVDEATA
                     AGPAYYMINCAHPNHFMSALPAGENWTKRVRGLRCNASKRSHQELNDSPDLDPGDPVE
                     LGDQYRDLLQRHPQINVLGGCCGTDHRHIACISRACRTATAAA"
     gene            6811..7410
                     /locus_tag="VF226_20280"
     CDS             6811..7410
                     /locus_tag="VF226_20280"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7076382.1"
                     /translation="MIVSSSAPTLTIRPARRDEAALLARLVNYAGEGLPLYLWSKLAQ
                     PGESAWDVGRKRAERDEGSFSWRNAIMVEVDGKAAGCLVGYETPDQPEPIPADMPAMF
                     RPLQELENLAPGTWYVNVLAVLPEHRGKGRGTKLLALADETGRKLGKRGMSVIVSDAN
                     FGARRLYERCGYRETARREMVKESWTNPWREWVLLTKPL"
     gene            complement(7501..8271)
                     /locus_tag="VF226_20285"
     CDS             complement(7501..8271)
                     /locus_tag="VF226_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076383.1"
                     /translation="MRLELRDLAFGYPEHPVGRDVNLTVEPGEVLCLLGPNGCGKTTL
                     FKTMLGLLRAQGGEVLLDGKPLNLFTRAEVARRIAYVPQAQTSPFPYTALDLVLMGRV
                     AHRGVFAGPTQEDRELAMAALDELGIGDLAHRDVTRLSGGQRQLVIIARAVAQAAPLM
                     VMDEPTASLDFGNQVVVLSDVKRLAARGVGVVLSTHQPDQAFAVADRVCLMRDGRIVA
                     MGPPVETLTSERLEDVYGVAVSIERTRSGQIVCAPRYG"
     gene            complement(8271..>9017)
                     /locus_tag="VF226_20290"
     CDS             complement(8271..>9017)
                     /locus_tag="VF226_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7076384.1"
                     /translation="PLVSPDVLGVSAGAGLGAVIGIFLSLPALMIQVLAFAIGLATVA
                     LVYLIAVAVRGRDPTLVLVLGGVVVGALAGAGISLLKILADPYDQLPAITFWLLGSLA
                     SIRGTELWNVLPAVLLGLLPMVLLRWRINVLSLGDEEAKALGVEAGRLRAIIIAAATL
                     MTASVVAIAGVIGWVGLVIPHIARMLVGPNFERLLPAAMLIGASYLVLVDTLARSMAQ
                     IEVPLGILTAVVGAPFFLWLLWRGRHGWSS"
BASE COUNT         1734 a         2952 c         2797 g         1534 t
ORIGIN      
        1 gggcctcagc gtgatgctgt tccaaaccgg cccgcttgcc tgacagccac gtcgaatccg
       61 gattgaagcc ggactcgtgc gtcgcgacgc tgccaaaacg attgcttgcc gaaccgcttt
      121 tttctcctct ccaggcacac ccgtccgcgg ccactgtgaa cgagccgacc caatggcgcc
      181 cggatcatcg gcctgttgat ttgccgagca tgggcgcgct caccaaatgt cattccggac
      241 gccgaaccct aacggagatg aacaatgcca acgtaccgca aggaccctga cgccattgcg
      301 aagctcaccc gggagcagta cagggtgacc caggagaacg ccactgagcg tcctggaacc
      361 ggcgaacatc ttcataacaa ggagccgggc atctacgtcg atatcgtatc gggcgagccc
      421 ctctttgctt cctctgacaa atatgagtca ggctgtggct ggccgagctt caccaagccg
      481 atcgagcccc tcaacatcaa tgaacggcgg gatacgtcgt acggcatgat ccgcaccgaa
      541 gtgcgctccg cacacggcga cagtcatttg gggcatgtct tcccggatgg cccgccggat
      601 cgcggcggcc tgcgctattg catcaactcg gcatcgttgc gcttcatcca ccgcgacgat
      661 atggaagccg agggctacgg ggcgtatctc gatcaagtcg aggatgtccg atgaggacgg
      721 acggtgcgcg ctgatcaagt tttgcgcggc agcgccggca atacccgctc gagaaatgcg
      781 tcgaatgcct gcatatagcc tcgcagaaat tcttctgtcg agggaacggt cacctcaccg
      841 tcatcagtga tcagaccagg cttgaagtgg atgtaagcct cgggtgcagt caattggggc
      901 gaattacagt agctcagaat ggcgcgcagg tcccgttgtg agagagcagt gcctatcgca
      961 ccgatcgacg ccccgatgac cgccgatggc ttgtttgcga aggaattcgt accgtaggga
     1021 cggctcgccc aatcgatggc attcttcagc gcgccgggga cggaccgatt gtactccggg
     1081 gtcacgaaca ggatggcgtc cgaagactca atcgcttttt tgaactggcg cgcgacttca
     1141 ggataatcgc tgtcgtagtc gtagctgtag aatggcagct ctttaaaaga gatctccgat
     1201 agctccagcc gctcgggcgc aagccgaacc aacgccttgg caagctttct gttgatcgat
     1261 tccttggcga ggctgccaac cagatagccg accttgaatt gcgccattgt cgtctccttg
     1321 cttcgggccc gcattcggaa atctagcact ttcggtaaat cacgattgga gcggcttcgt
     1381 tccgaccgtt agccttgggc gatggccgtt gtggccacgt cggagcgagc gtccgaggcg
     1441 ctgcgaccgc ctatcgcagg tgatgtttgt ggcagcgcaa agatgaaatt gtgcggtgca
     1501 cctattttcc ttcgtaacaa gacggccaaa ggtcacgaag gagacaggtc atgttcacca
     1561 ggcgcgagac actcgtaggc gttgccggcg cggcaatcct tgcggcgatg ccgaagacgc
     1621 cagttgcggc agagcagatc gacgtcgctc gcctgccccg caagaaggtg acactgctcc
     1681 caccgccgtt cgtgcacgca cacgatcagg tggcaaaggg cggaccgatg atcgtcgagt
     1741 tcacgatgac cattcaggag aagccggtgg tcatcgacga tgacggcacg accatgcagg
     1801 ccatgacctt caatggctcg atcccaggcc cgatgatggt cgtccacgaa ggcgactacg
     1861 tcgagctgac gctcatcaac ccggcgggaa acgagatgcc gcacaacatc gacttccacg
     1921 ctgcgaccgg cgcgctgggc ggtgctgcgc tcacgcacgt caatcccggc gagcaggtca
     1981 cgctgcggtt caaggcgacc cggagcggca ccttcgtcta tcactgcgcg ccggaaggca
     2041 tgatcccctg gcacgtcgtc tccggcatga gcggcacgct catggtgctg ccgcgggacg
     2101 gcttgaagga cgaaagggga cggccgatcc gctacgacac cgtttattac atcggcgaaa
     2161 acgagttcta cgtcccgcgg gacgagaatg gaaacttcaa gaccttcgag agcctcgccg
     2221 acagctatgc cgataccatc gaggctatgc gcggcctgat cccgacccat gtcgttttca
     2281 atggccgggt cggcgcgctg acgggtgagg gtgcgctgaa ggccaaagtc ggagagacgg
     2341 tgctgttcgt ccactctcag gccaaccgcg acacgcggcc tcacctgatc ggcggacatg
     2401 gcgactacgt ctgggaggag ggcaagttcg ccaacccgcc ggccaaggac ctcgagacct
     2461 ggttcatccg tggcggatcc gcaggcgcgg cgatctacac gttccggcag ccgggcattt
     2521 acgcctacgt gaatcataac ctgatcgagg cggccgagct gggtgccacg gcgcacatcg
     2581 tggtcgaggg cgagtgggac gacgacctga tgacccaggt caaagcacct ggtccgatca
     2641 ccaccaacta gcctgattgg gcgggacgcc cacggctttg cggcggcccg ccttccactc
     2701 gaaggcgggc cgtttccatc gaggaaacca gcgctatgaa gctctctgtt cccattgccg
     2761 cagcggcagc ggcctcggtc ctcgcggcag gcttaattct gcaggcgccg ccccgccggg
     2821 caacgacgga tctggcgatc aagacgatca cgatcgagcc cgaacccttc caataccggc
     2881 tcccgggcga gttcctgaaa aacggtcgga tcgccgacgg ccccaagcag agcacggcct
     2941 tgtcccggcc gctggaaatc atggcctatc aggtgagcgc agcagagtac gcggcctgtg
     3001 ttgccgatgg tgcctgccgg cccgcagatg gacccggcac gccaagcgat ctgcccgtaa
     3061 ccggcgtcag cttccacgac gccgaggcct atgccggctg gctttcggaa aagacggggg
     3121 aacgctggcg cctgccgacg gatgaggaat gggcctttgc cgctgcggag cgcttcagcg
     3181 acgattcgct cgagatcgtc gagaccgacg cggccaatcc ggcagcccgc tggatcgccg
     3241 cctatcgcga gcgcagcgtg ggcaagagcc gggatacgac gccgaaaccg cggggcactt
     3301 tcggcgccaa cggccgcggg ctgatcgacg tcgccggcaa cgtctgggaa tggaccagca
     3361 cctgctatac acgcgcccac atcgatgcag acggcgagat cgtccaaacc accgagaatt
     3421 gcggtgtgcg cgtcacgggc ggccgccatc gcggctatat gacgagcttc ctccgcgacg
     3481 gcaaaagcgg cggctgtgcc gccggcatcg cgccggacaa cctgggcttc aggcttgtca
     3541 aggatcccgc gccgaccatc gcgcagcgcc tgctcgggcg attttccaga agctgactta
     3601 attcgcggtc taacctgacc gcgaattcgc attatccttg cttctccgtt cacccgaccg
     3661 ccatgttcgc gcaatggatc cggcgcgccg cagccggaat gccgtgtcag gttttgcagg
     3721 cgtgcccttt accagacatg ccgcgccaat cggctcgtga tgccgatgcc ctccttgcgt
     3781 gaccgtggct aaaggcgcgg gttgtgaggt taccagcgcg accgccagct taaaagaaaa
     3841 ccgagcggct caatcaagtc gcccggtacg gtagctcgtg cgtccagaat tgttaatcag
     3901 cgggcagctc tgcaaagagc tcctcgaaaa ccttcttggc cttgccgggg tgggataccg
     3961 cctttgcagc gtcgagattg acgggatcgc cgaccacgat cagcgccacc atgcccatcc
     4021 cgtaatgggg catgcatttg acgccgtaaa cgccttcctt cgtcagcgtg acggtgaact
     4081 cctcgttgat cttgcttttg aacccctccg cgccttcggg aatcatgcct ttgatggctt
     4141 ctgcgttgtg ggatttgtcg gtgggcacga accggatcgt gtcgccggga gcggctttca
     4201 ccagatccgg ctcgaatacc atggcgccct tttggccctt gttgagcatg tggatctcgt
     4261 gctccgccgc ctgggccccg cccgcgagca gcatcgtcgt gagcgccgcg aaggcaatca
     4321 tccgcttcat gatcagctcc cttttttcct catgaaagat cgctctcgtg ccatggtcct
     4381 gatagggctg gagcgatggc aggacgttgc ggaagcacaa acaaaaacca aattgcctgc
     4441 gccacgacgc tacttgcgac cagcatcatc gcatggaaca ttcgcgtgag atgaggagga
     4501 cgccgcttga gtcgccttga ccgctccctg atcgcgggac tgcccctgtt ttcaggcatg
     4561 tcgccggccg atctcgacaa gatgctcgcc acggccacgt cccgccgcgt gccgaaggat
     4621 ggttacgttt tcgagcagga cgccgaggcg aaatccttct tcgtcctcct caatggccgg
     4681 gtgcgcgtcg tcaagaccac gccggacggc catcaggtga tcgtgcgcta catcggggcc
     4741 ggagagctgc tcgggatcgc tcacgcgatc ggccgcacga cctatccggc aagcgccgtc
     4801 gctgtcgtcg attgcgtcgt gctcgcatgg ccgagccgcc tgtgggcgga gttcgccacg
     4861 gccttcccga gcttcggtgc caatacctac aagaccgtcg gcgagcggct tcaggatgtc
     4921 cagtcgcagg tggtcgagct ggcaacccag caggtggagc ggcgggtggc ccacgcgctt
     4981 ttgcggctcg tccagcagac aggcaagaag tccgacgaag ggttgctgat cgactttccg
     5041 atttcgcgcc aggacatcgc cgaaatgacc ggggcgacct tgcacacggt cagccgcctg
     5101 ctgtcggcgt gggagcagca gggtctcgtc aaaagcggcc ggcagcggat caccgtggtg
     5161 gaaccccatc gcctgctgct gatcgctgag ggacggtcgg aaaagagctg acgggcgtcc
     5221 ggctagttga tcgcggccat aagctcgcgg cggaacctct cactgtcgag tccatgctcg
     5281 cggatggcat ccgcgaccgt gtgaaaaccg gcaatcgggc aacccacaca aagcagctga
     5341 tggcgcagca cgacgcggat cgtcgccggc caccgccgca tgatttcatc cattgtcatg
     5401 tcgtcgcggt atcgtcccgt catccaagcg cctccggtga tgggccgaca accatgatcc
     5461 cggtccggcg ctccttcttt gcgcagcgac aaacaatttt tgcgtacggc ggcgcacagg
     5521 cgatctggcc gtttctcgtc tgttcgatcg ggcggcgacg atgcggcagc cccgcataag
     5581 acagatccgg tctggggctt cgaaggcctg atcccggctg ccgatagaag cgatcaccag
     5641 caacgacgaa agagcctgtt ccctgcggaa cagcaccagc tccgccgatc tgcttttggt
     5701 ccgcccgcga atttggcgcc ggccaaatcg cgttgattga tatcgatcat gagacagcaa
     5761 ggaaaaccca tgttggagaa ctccagcaat cgcctaccgc agctcgacgg cggcttgttc
     5821 cttactgacg gcggcatcga gacgacactg atcttccacg aggggctcga cttgccctat
     5881 ttcgccgcct tccacctgat gaaatcgcaa gaaggccgcg ccgccctcgt ccgctatttc
     5941 gagcaatacg ttgccatcgc ccgcgccgac ggaacgggct tcatcttcga gagcccgacc
     6001 tggcgcgcga gcagcgactg gggcgcgctc ctcggctact cccgcaatga catcgcggca
     6061 gtgaaccgcg agtccatcgc gctcatgcgg gagctgcagg cgaagcacgg cgttccgcag
     6121 ctccccatgg cggtgagcgg ctgcgtcggt ccgcgcggcg atggttacga tcccgggcag
     6181 gtaatgtccg ccgacgaggc ggagagctat cacgcacacc agatcggcgc ctttgccgag
     6241 gctgaggccg acatggtgac cgccatcacc atgacgaaca ccaacgaggc catcggcatc
     6301 acgcgcgccg ccatgaaagc tggcatgccg gtcgccatct cgttcacgac ggagacggat
     6361 ggccgcctac cgacaggcca gagcctcgcc gatgcgattg ccgaggtgga tgaggccacc
     6421 gcggcaggcc ctgcctacta catgatcaac tgcgctcacc ccaatcattt catgagcgcg
     6481 ctgcctgcag gcgagaattg gacgaaacgg gtccgcggcc tccggtgcaa cgcgtcgaag
     6541 cgcagccacc aggagctcaa cgactcgcca gatctcgatc cgggtgatcc ggtcgagctc
     6601 ggtgatcagt atcgcgatct gctgcaacgc catccgcaga tcaatgtcct gggtggctgc
     6661 tgtggaacgg atcaccgcca catcgcgtgc atcagcagag cctgccggac ggccacggca
     6721 gccgcgtaag cttcgcccgg ggcccggtcc ccactacccc ccgctcgacc ccgtggtcgg
     6781 gctccgggcg atccttcctg ggaaattccg atgatcgtca gcagttcagc accaaccctt
     6841 acgatccgcc ccgcccgccg cgacgaggcc gctcttcttg cgaggctcgt caactacgcg
     6901 ggcgaaggac tgccgctcta tctctggtcc aagctcgccc aaccagggga gtcggcctgg
     6961 gacgtcggcc gcaagcgcgc ggaacgcgac gaaggcagct tctcgtggcg caatgcgatc
     7021 atggtcgagg tcgatggcaa agcggccggc tgcctggtcg gctatgaaac tcccgatcag
     7081 ccggagccca tccccgcaga catgccggcg atgttcaggc ccttgcagga gctggaaaac
     7141 ctcgcaccag gcacttggta cgtaaacgtt ctggctgtgc tacccgagca tcgcggcaag
     7201 gggcgcggaa caaaattgct cgcgctcgcc gacgagacgg gccgcaagct tggcaagagg
     7261 ggcatgagcg tgatcgtctc cgatgccaat ttcggcgccc gccggctcta cgaacgctgc
     7321 ggctatcgcg agaccgcacg gcgggagatg gtcaaggaga gctggaccaa tccctggcgg
     7381 gagtgggtgt tgctgacaaa gccgctctga ccccccggtc gagcgcacca catccccctg
     7441 ctcgcgcggc ggcctcgcgt ttcgcgtacc gccgcgcggg caccgcaaaa cggctcaacc
     7501 tcacccgtac cgcggcgcgc agacaatctg gccactgcgc gtgcgctcga tcgagacggc
     7561 tacgccatag acatcctcga ggcgctcgct cgtaagcgtt tcgaccggcg gccccatggc
     7621 gacgatcctg ccgtcccgca tcaggcagac gcgatcggcg acggcaaagg cctgatccgg
     7681 ctgatgggtc gacagcacca cgccgacgcc ccgcgccgcc agccgcttca cgtcgctcag
     7741 cacgacgact tggttaccga aatcgaggct cgccgtcggc tcgtccatga ccattagcgg
     7801 tgcggcctgg gccactgcac gcgcaatgat gacgagctgg cgctgcccgc cggaaaggcg
     7861 cgtcacatcg cgatgtgcga gatcgccgat gccgagctca tcgagcgccg ccatcgccaa
     7921 ctcgcgatct tcttgcgtcg gaccggcgaa gaccccgcga tgcgcgaccc ggcccatcag
     7981 gacgaggtcg agcgcggtat agggaaacgg gctcgtctgc gcctgcggaa cgtaggcgat
     8041 cctccgcgcc acctcggccc gcgtaaacag gttgagcggc ttgccgtcaa ggagcacctc
     8101 cccgccctgt gcccgaagca agcccagcat ggtcttgaac agcgtcgtct ttccgcagcc
     8161 gttaggtccg aggaggcaca gcacctcgcc cggctcgacg gtgaggttca cgtcgcggcc
     8221 gaccgggtgc tccgggtagc cgaacgccag atcgcgcagc tcgagccgca tcacgacgac
     8281 cacccgtgcc ggccgcgcca tagcagccac aggaagaagg gtgctccaac caccgccgtc
     8341 agtatcccga gcggcacctc gatctgggcc atggagcgcg ccagagtatc gacgaggacc
     8401 aggtagctgg cgccgatcag catggccgcg ggcaacagtc gctcgaagtt cgggccgacg
     8461 agcatgcgcg cgatgtgcgg gatgacgaga ccgacccagc cgatcacccc ggcaatggcc
     8521 acgacgctcg ccgtcatgag tgtcgccgcg gcgatgatga tggcgcgcag gcggccagcc
     8581 tccaccccga gcgccttagc ctcctcgtcg ccgagagaca gcacgttgat ccgccagcgc
     8641 agcagcacca tcggcagcag cccgagcagc accgctggca gcacgttcca cagctcggtg
     8701 ccgcggatgc ttgcgaggct gccgaggagc cagaaggtga tcgcggggag ctggtcgtaa
     8761 ggatcggcga ggatcttcag cagcgagatc ccggctcctg ccagggcacc gacgacgacg
     8821 ccgccgagca caagcaccag cgtggggtcg cgcccgcgca cggcgacggc aatcaaatag
     8881 accagcgcca cggtcgcgag cccaatcgcg aatgccagga cctggatcat cagcgctggc
     8941 agcgacagaa aaattccgat gaccgcaccg aggccggcac cagccgagac accgagcaca
     9001 tccggcgaga ccagcgg
//
LOCUS       DASVDU010000302         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_996655, whole genome shotgun sequence.
ACCESSION   DASVDU010000302 DASVDU010000000
VERSION     DASVDU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996655"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..909)
                     /gene="ligD"
                     /locus_tag="VF226_20295"
     CDS             complement(7..909)
                     /gene="ligD"
                     /locus_tag="VF226_20295"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX7076385.1"
                     /translation="MAPGTANEIALKGVRLTSPEKVLYPEQGVSKRELADYYCTIADD
                     ILPHVARRPISLVRCPDGRQRQCFFQRHAGSGVPPEVSEVDIPGFEDSGAYLYIKDVR
                     GLIALVQMGVLEIHPWGSRIDRPDRPDRIVFDLDPGEGLGFRDVVAGAHDLRAVLRDR
                     SLESFAKTTGGKGAHVVVPIARRYEWPEVKAFAKAVAEGMAADAPDRFLTKIPKAQRK
                     GRIFIDYLRNDPTSTAIAPYSTRARAGAPVALPLEWDELRADLDPAQFNIHTVPERIA
                     RRNSDPWAEIGRLHQSLPKSVLRQ"
     gene            complement(911..1864)
                     /locus_tag="VF226_20300"
                     /pseudo
     CDS             complement(911..1864)
                     /locus_tag="VF226_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
     gene            2137..>2677
                     /locus_tag="VF226_20305"
     CDS             2137..>2677
                     /locus_tag="VF226_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076386.1"
                     /translation="MKYATIAGSRASINTRPQIITSRWSGRRQTIFRAAGDGKPSTTL
                     SNVALSDTRLSCEALGALARIMKLPDDWTFNVAWLCRTFGMGKDRAWRILRELETNGY
                     MKRAQQRDANGRLGRAEYLFTDDPATWHDLAELPHPEKPCTVNPPPTKNRDLQDRSAP
                     DAPEVSEEAITEAAAMAPHV"
BASE COUNT          496 a          853 c          849 g          479 t
ORIGIN      
        1 cgcacgtcat tgacgcagca cactcttcgg cagcgattgg tggagccggc cgatctccgc
       61 ccaggggtcg ctgtttcgcc gcgctatccg ttccggcaca gtgtggatgt tgaactgcgc
      121 gggatcgagg tcggctcgca gctcgtccca ttccagcggc aacgcgacgg gcgctcccgc
      181 ccgggctctc gtcgagtaag gcgcgatggc agtggaggtg ggatcgttgc gcagatagtc
      241 gatgaagatc cgccccttgc gctgcgcctt cggaattttg gtcaagaacc gatccggcgc
      301 atcggcggcc attccttctg ccacggcctt ggcgaacgcc ttgacctccg gccattcgta
      361 gcgcctcgcg atgggcacaa cgacatgcgc gcctttgccg ccagttgttt tggcaaatga
      421 ctccaggcta cgatcgcgga gcacggcgcg gagatcgtgg gctccggcta cgacatcgcg
      481 gaagccgagg ccctcgcccg gatcgagatc gaacacgatg cggtctggcc ggtccggccg
      541 gtcgatgcgc gaaccccacg ggtgaatctc caggacaccc atctgcacca acgcgatcag
      601 ccccctcaca tctttgatgt agagataagc gcccgagtcc tcgaagcctg gaatatcgac
      661 ctcgctgact tccggcggca cgcccgagcc ggcatggcgc tgaaagaagc attgcctttg
      721 gcgcccgtcc ggacagcgga cgaggctgat cgggcggcgg gcgacatgcg ggagaatatc
      781 atccgcaatg gtgcagtagt agtcggcgag ctcgcgcttg ctgaccccct gctcggggta
      841 aagcaccttt tcgggactgg tcaggcgcac gcccttgagc gcgatctcgt tcgcggttcc
      901 tggtgccacg tcagtgctcc agtagctcgg caatgacctg cgggctcggc tcgcgctcga
      961 gctggtcgaa cgtgcattgc tggggttcct tgtccggccg ccatcggaga aggcgcgttc
     1021 cgtggcggaa ccggttgccg gtcgtgtgat cgtagcagac ctcgaccaca agctctggcc
     1081 gcagcggctc ccattcgccc gaccgctcgg tgctccagcg gctcggtccg ccgggcgcat
     1141 cgccggtgaa gcccggagac tcgaccaggg cttcaagccg gcgtgtcagc tccgggcgct
     1201 cggcatggct gatgccggac gtgaagccga cgtgatggag caacccattc tcgtcgtaga
     1261 gcccgagcag cagagagccg accagacgcg aacccgtctc gtaccggaag ccaccgacga
     1321 cgcagtctgc cgtgcgcatg cgcttcacct tcagcattgc ccgttcgccc ggccggtacg
     1381 gcgcatcctc tcgctttgcc acgaccccgt cgagtgcgcc gccgcttttt tcgagccagc
     1441 gtcgggcttc cgatcggtcg agcgtatagg gcgagagcca cagccattcc tggtgcggaa
     1501 ccgaccggta gaaggcttcc agggcctgcc gccgttcagg gagcggagag tcgagcagac
     1561 tgcggccact ggcatcgagc aggcaatcga agatgatgaa ggtggccggc gtttcggcgg
     1621 caagccgctt gatcctgctt tccgccggat gcaaacgcag ctgcagtgcg tcgaaggaaa
     1681 gtgtgccgtc ccgagcaatc gcgagttctc cgtcaatcgc gaacctgggc tcgggcaagc
     1741 cttgcagcag tgctctgatc tcaggaaaaa accttgtcag gtcgcgtccc gatttcgacc
     1801 gcaaagtgac ctcggaaccg ttgcgaaagg ccaggcagcg gaagccatcc catttcggct
     1861 cgaaaagtaa agaaccaatc acatcaaaag gttagcggat ggtgtgaaca aatttattcc
     1921 ccccaatatg gtggggccgc cccggagcgt gttcccactg ttgcagggct agtcgtctct
     1981 gaaaacgacg agggcgaggc accgttcccg cggtcctcgc ccatttcgtt gcgtcggccc
     2041 gccttggcga gcgtgccgat gatctgtgtg cggttctaac tcaacctgcg gccacctgtt
     2101 ggcgcaggtt ggccgcgtac agaaggcatt tctgctatga agtacgcaac tatagctggt
     2161 tcgcgggctt cgatcaacac gcgcccgcag atcatcacca gtcgctggtc tggacgtcgg
     2221 caaacgatat tccgcgcggc cggcgacggg aagccgagca ctacgctcag caacgtggct
     2281 ctgtccgata cgcggctgag ctgcgaggcg ctgggggcgc tcgcgcgaat aatgaagctg
     2341 ccggacgact ggacgttcaa cgtcgcttgg ttatgccgca cgttcggcat gggcaaggat
     2401 cgcgcctggc gcatcctccg cgaactcgaa accaacggct acatgaagcg agcccaacag
     2461 cgggacgcca atgggcgcct aggccgtgct gagtaccttt tcaccgacga tccagcaacg
     2521 tggcacgacc tggccgagtt accgcacccg gaaaaaccgt gcacggtaaa cccgccccct
     2581 acaaagaata gagatctaca agatcgatca gcgcctgacg cgccggaagt gtcggaggag
     2641 gcgatcaccg aggcagcggc gatggcgcct catgtcg
//
LOCUS       DASVDU010000303         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_190951, whole genome shotgun sequence.
ACCESSION   DASVDU010000303 DASVDU010000000
VERSION     DASVDU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190951"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1643)
                     /locus_tag="VF226_20310"
     CDS             complement(213..1643)
                     /locus_tag="VF226_20310"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7076387.1"
                     /translation="MDISRFERLSDTAWQIPPTGAMRVPGVIYATEELIRDMDDKVYD
                     QVTNVATLPGIVKASFAMPDAHWGYGFPIGGVAAFDPDDDGVVSAGGVGFDISCGVRT
                     HLTGLSRSDIMKVQGELADALYAQVPVGVGSEGKITLDDAEMTAMLAGGAHWAVARGY
                     GRPDDLERIEENGRQEDAVPSAVSQQARKRQRRAMGTLGSGNHYLEVQEVAEVFDAGA
                     AEAFGLKEGECVVTIHCGSRGLGHQIGTEFLREMAVAAATYGITLPDRELACAPIKSE
                     VGQRYLGAMRSAINCALANRQIIGHLARQVFRRIFGEPDLPLLFDVSHNTCKVEPHAV
                     GSVARHLYVHRKGATRAFGPDHPNLPEALRRWGQPVFIGGSMGTESYVLVGTRESEEL
                     AFSSACHGAGRQMSRSEALRTWSGREVLDRLKARGILVRSPSLRGVAEEAPGAYKDVR
                     AVVDAAENAGLARKVARLAPLICIKG"
     gene            complement(1665..2114)
                     /locus_tag="VF226_20315"
     CDS             complement(1665..2114)
                     /locus_tag="VF226_20315"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX7076388.1"
                     /translation="MSEAGDGSSTGLAHWEHFQHGADIGVRGIGPSLASAFEQAALAL
                     TAVLVNPGTLQPSETVEIELEAPNADLLLYDWLNALVFEMGTRGLIFGAFDVAIEGNK
                     LVARAHGERVDRDRHQPAVEIKGATMTELSAGEVHPGQWRAQCVVDV"
     gene            2539..3504
                     /gene="glk"
                     /locus_tag="VF226_20320"
     CDS             2539..3504
                     /gene="glk"
                     /locus_tag="VF226_20320"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX7076389.1"
                     /translation="MASREVLVADIGGTNARFSVADQDTLQLRYFATYRCAEFPSLTE
                     VARKYLSGLPKPPKLAAVAVAGPTGGEVVQLTNSTWKFRVSELRAALGLHELLVLNDF
                     EALAYALPCLEASDLHQLGGTEPLEHAAKAVLGPGTGLGVAGLAWSPSGWLAVPSEGG
                     HSTFAAKDARELAMVERLIAGRGHLSVERVVSGPGIVDVYRALAEMHGHTAPQLQPAE
                     VVTRARSEADPLAREAIELFVTWLGRFAGDVALFFGARGGVYLGGGIAPKLIDFLSAG
                     EFRRSFEAKGRMASYLTPIPVYVILTGDAALKGAAAALATRFQEA"
     gene            3573..>4151
                     /locus_tag="VF226_20325"
     CDS             3573..>4151
                     /locus_tag="VF226_20325"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX7076390.1"
                     /translation="MCNLYSMTRAPEAVRRLFRVAHNRAAAFEPLRAIFPGHLAPIVR
                     KAADGEREIAILSWGFVLPQNGRAPRRVTNVRDDKVLSSSFWRESFELRRCLVPASSF
                     CEPKGERPATWHWFALRGEEPRPLFAFPGLWRRWRGPLKSGGPIVELDVFSFLTTRPN
                     ELVATINHERMPVLLNTQDQFETWLSGSPQEAL"
BASE COUNT          741 a         1375 c         1287 g          748 t
ORIGIN      
        1 gcgcgaactc gtcgatgaag cttgcaacgt ccgccacgcg catgaccagc acattgaaaa
       61 acggggtccg ctccacatcg ccgaacttgc gcaacgcacg tttggcttca cggcgcccca
      121 tcgcgtcgta aaccgtcaca acgacgttcc aatctctcat tggcgacgct tccggcagag
      181 ctgctgcaaa cgcccccgcc gtcaaatcgg cctcaacctt tgatgcagat gagcggcgcc
      241 agccgcgcca ccttgcgggc aagtccggca ttctcggcgg catcgacaac agcgcgcaca
      301 tccttgtagg caccgggtgc ttcctcagcg acgccgcgaa gcgacgggct gcggacgagg
      361 atgcctcgag ccttcagtct gtccaggacc tcacgtccac tccatgtccg cagcgcttca
      421 ctgcggctca tctgccgccc ggcgccgtgg caggccgagg aaaatgcgag ctcctcgctc
      481 tcgcgcgtgc cgacgagcac gtaggactct gttcccatgc tgccgccgat aaacaccggc
      541 tggccccacc gccgcagcgc ttcgggcagg ttggggtgat ccggaccgaa cgcgcgcgtg
      601 gcgcccttgc ggtgaacgta gagatgacgc gccacggatc cgacggcatg cggctcgacc
      661 ttgcaggtat tgtgcgacac gtcgaacagc agcggcagat ccggctcacc gaatatgcgg
      721 cggaacacct gccgtgccag atggccgatg atctgtcggt ttgcgagcgc gcagttgatc
      781 gccgaccgca tggcgccgag gtaacgctgg ccgacctcgg acttgatcgg cgcgcaggcg
      841 agctcccggt cgggcaatgt tatgccgtag gtcgcagccg cgactgccat ctctctcaag
      901 aattccgttc cgatctggtg gccgaggccg cgtgagccgc agtggatggt gacgacgcac
      961 tcgccctcct tcagtccgaa ggcctccgca gcaccggcgt cgaagacttc agcgacctcc
     1021 tgcacctcca ggtaatgatt gccagatccg agcgtgccca ttgcgcgccg ctggcgcttt
     1081 cgcgcctgct gggagacggc agacggcacg gcgtcctcct gcctcccatt ctcctcgata
     1141 cgctccagat cgtccggccg gccgtagccg cgcgcaacag cccagtgggc gcccccggcc
     1201 agcatggccg tcatttcggc gtcgtcgagc gtgatcttgc cctccgagcc gaccccgacc
     1261 ggcacctgcg cataaagcgc atcggcaagc tcgccctgca ccttcatgat gtcggagcgg
     1321 gacaggccgg tgagatgtgt gcgcacgccg cacgaaatat cgaatccaac tccgccggca
     1381 gagacaacgc cgtcatcatc cgggtcgaac gccgcaacgc ctccgatggg gaaaccgtag
     1441 ccccagtgcg catccggcat cgcaaaagaa gctttgacga tgccaggtag cgtcgccacg
     1501 ttcgtcacct ggtcatagac cttgtcgtcc atgtcccgga tgagctcttc ggttgcatag
     1561 atcacgccag gaacgcgcat cgcgcccgta ggcgggatct gccaggcggt atccgaaagc
     1621 cgttcgaaac gcgaaatatc catatcagct ccttccgctg gagctcaaac gtcgacaacg
     1681 cactgggccc gccattggcc gggatgaacc tcaccggcag aaagctctgt catggtggcg
     1741 cccttgatct cgacggcggg ctgatgccgg tcacggtcga cgcgctcccc atgggcgcgc
     1801 gcgacgagct tgtttccctc gatcgcaacg tcgaaggcgc caaaaatcag tccccgcgtg
     1861 cccatctcga aaacgagcgc attcagccag tcgtagagca gcagatcggc attcggcgcc
     1921 tcgagctcga tctcgacggt ttcagacggt tgaagggttc cgggattgac gaggacggcc
     1981 gtgagcgcca aggccgcctg ctcgaaggct gaggcaagcg atggcccgat cccgcgcacg
     2041 ccgatatctg cgccgtgctg gaaatgctcc caatgtgcca gacccgtgct ggacccgtcg
     2101 cccgcctcgc tcacgtcgca attcctcagg cttcaaaacc tgccatccgc aactgcacaa
     2161 ccggagaaaa ctgcttaagg ttctccgcga ataacggtca caatcccaga ggcctggctg
     2221 aagtggggcg cgcgtcgcac tcaggctacg gaacggatga tgacgggcac ccaggccgca
     2281 tctacgcaag gtgcccgatc tggggctacg ccaagagtga gggaggagca cccctgccgt
     2341 ggccaacttg gcattccaac gaattccggg acaactggtt ccaattattc gccgaatcca
     2401 atagcccatg ttcggagctt acatcgggcc aatgcttcga gtgatgtagc gcggttggac
     2461 attctgaacc gggtccgcct cgatggggct cggcgatcgt tatctctgag gagtagtcca
     2521 tcgaaacgga ggttcggcat ggcttcgcgt gaggtgctcg tcgccgacat cggcggaacc
     2581 aatgcgcgct tttccgtggc tgatcaggat acgctgcagc tcagatattt cgccacctac
     2641 cggtgcgcgg agtttccatc gctcaccgag gtcgcccgca aatatctgag cggcctgccc
     2701 aagccgccca aattggcagc cgtggcggtc gccgggccga caggcggaga agtggttcag
     2761 ctcacaaatt ccacttggaa attccgagtg agcgagctcc gcgccgcttt gggattgcac
     2821 gagctcctgg ttctcaacga cttcgaagca ttggcttatg cgctgccctg cctcgaagcc
     2881 tccgatctcc atcagctcgg cggcaccgag ccgctggaac atgctgcgaa ggcggtgctc
     2941 ggtccaggaa caggactcgg agtcgcgggt cttgcctggt cgccgagcgg gtggctggcg
     3001 gtgccgagcg aaggcggtca ctccacgttc gcggcgaagg atgcccgcga gctcgccatg
     3061 gtcgagcgcc tgatcgccgg caggggtcac ctctcggtcg agcgtgtcgt ctcaggcccc
     3121 ggcatcgtcg atgtctaccg cgccctggca gaaatgcacg ggcataccgc accgcagctg
     3181 caaccggccg aagtggtcac acgcgcgcgc tcggaggcgg acccgctggc ccgagaagcg
     3241 atcgagttgt tcgtgacctg gcttggccgc ttcgcaggcg acgtggcgct gttcttcggg
     3301 gccaggggtg gtgtctacct cggcggcggg attgcgccca agctcatcga ctttctttcc
     3361 gcgggagaat tccgccggtc gttcgaggca aagggtcgga tggcgtctta tctgacccca
     3421 attccagtct atgtcatcct aaccggcgat gcggctctca aaggggctgc cgcagcgctc
     3481 gccacgcggt ttcaagaggc ctagatcggg ggcagctttg cgagaccagc cagagccttg
     3541 cgccaccgtc cgttgcgcag aatattgccg ctatgtgcaa tctctactcc atgactcgag
     3601 cgccggaggc tgttcggcgg ctatttcgcg tcgcgcacaa ccgggctgcg gcattcgagc
     3661 cgctgcgggc gatcttccct ggccatctcg cgccgatcgt acgaaaggcg gccgacggcg
     3721 agcgggaaat tgcgattctg tcctggggct tcgtgctccc gcagaacggc cgggctccaa
     3781 ggcgtgtcac gaacgttcga gacgacaagg tgctctcgag ctccttctgg cgggaatcgt
     3841 tcgagctgcg gcgctgcctc gtgccggctt cctccttttg cgagcccaag ggggagcggc
     3901 ccgccacctg gcattggttc gcgctgcgcg gagaggagcc gcggccgctg ttcgccttcc
     3961 ccggcctgtg gcggcgctgg aggggaccgc tcaagtctgg tggccccatc gtcgagctgg
     4021 atgtcttcag ctttctgacg acgcgaccga acgagctcgt cgccacgatc aatcacgaac
     4081 gcatgcccgt gctgctcaat acgcaagatc agttcgagac ctggctcagc ggctcaccgc
     4141 aggaggcgct c
//
LOCUS       DASVDU010000304        16832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1246400, whole genome shotgun sequence.
ACCESSION   DASVDU010000304 DASVDU010000000
VERSION     DASVDU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16832
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246400"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF226_20330"
     CDS             <1..207
                     /locus_tag="VF226_20330"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX7076391.1"
                     /translation="LAKINEHLQNIRPLGADEAARAVFRVLDKNIPAGEMNEVKQMLP
                     PEIRTLFPGSAGPSAAQQQVPLDR"
     gene            complement(210..1334)
                     /locus_tag="VF226_20335"
     CDS             complement(210..1334)
                     /locus_tag="VF226_20335"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEX7076392.1"
                     /translation="MTDTTIAALKTTLLRIPWAGEPPANGIMPPTHRELLVLEISTRG
                     GITGMAYLQPLAGGLETIDACLKELIAPKVLGRDATEVEGIWQTLWTSTYWVGRAGIS
                     VFALSAVDIALWDIVGKRAGLPLYRLWGKCRDDVPAYGSGCWRGLGRDGMIARAKHFT
                     GMGMQAIKMQVAHIRPWREDVANVRAMREALGPDIEIMIDVNMGWSADTAIQAGRYFD
                     DLDVYWLEEPVVCEDFEGYQRVAAALKTRVVGGESHFTRYDLRPFFTRPSTPILQPDP
                     MRGGLTELRKIAAVAETWGIAIAPHLFHELNVHLLASIPNASWLEYMDWNDDLWIEPA
                     IPVDGKLRPPERPGHGLAFKPEVLTEHRVGGFEVTLSRAS"
     gene            1565..2542
                     /locus_tag="VF226_20340"
     CDS             1565..2542
                     /locus_tag="VF226_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7076393.1"
                     /translation="MSFVKFAGLAISALGLVAAGTASAQTTFKMNISVPQDSHYGVAV
                     DTFAAEVEKRTNGRYKIQNFYASALGNEREAIEAVQLGTLDMAMSSTGPVPNFVPELA
                     ILDIPFLFRDYAHARATLDGPIGQEMLKKFEPIGIKALAWGENGFRHMTNSQRPVRTP
                     EDLKGLKMRTMENPIHMQAYKGFGIIPTPMAISEVFTALQQGTVDGQENPISVIQSWK
                     FDQVQKHLTLTGHVYSPAFLMMNKGLWDGLSDEDKQHFLDAAKEAVKANRARIDQDEK
                     NGVAYLRSKGMEVIDDVDRTQFQKALEPVFAELAKKFGEENIRRLREGK"
     gene            2646..3140
                     /locus_tag="VF226_20345"
     CDS             2646..3140
                     /locus_tag="VF226_20345"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX7076394.1"
                     /translation="MRDIILTIERHTTGFAMWCAGLMLVIASLAGFYQIISRFVLEQP
                     AEWSEVTVRFSLIWMVFLGIPTAFRQGAMLSVDLLYRKSGPRLRRALDWVIFLASLVL
                     MLAILGWGYDYTYRTRFQTIPGLEFLTMVWAYSAMPVGAAFSLLAILGQLLDPRHREL
                     ETAQ"
     gene            3260..4531
                     /locus_tag="VF226_20350"
     CDS             3260..4531
                     /locus_tag="VF226_20350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7076395.1"
                     /translation="MSIAMLITMCLGFALSISIAVSIGLAALLGTWLSNVNMLLSVKE
                     IYTAINKFPLAAIPFFILAGNIMESGGISRRLVEFAKSVVGGVQGGLSATCVLTCMIF
                     AAVSGSSVATTFAIGSILVPALVRHGYPVNYAAALQATSAELGVVIPPSIPMIIFGVS
                     AEVSIGELFIAGIGPGILIAGGLISFVLIYALIKGYGKQDREGRLPLLTATRQAMFAL
                     FMPVIVLGGIYGGVFTPTEASVAAVFYALLVGMVVYREIGLKDLGLILRKSVISSSVI
                     LFIIANAGLFSYLITRAGVPDQIGEFLRANLGTPFMFLLGVNTALFFIGMFIETSAAI
                     IVLAPILVPVAAGFGIDPVHLGVIVVVNLALGMITPPFGVNLFAACAVAKISVDQIIL
                     RLVPFVLVVLVCLMIITYIPPISLFLRDLMY"
     gene            4595..5497
                     /locus_tag="VF226_20355"
     CDS             4595..5497
                     /locus_tag="VF226_20355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7076396.1"
                     /translation="MDNQAWMEALRDFAATSRFREGGGIVTDLDGTAVHEHEGRIVIP
                     HNVSHALQRLNEQGHPIAINTLRFPMSVIGTFGQEWYAITNAPLPLISLNGSLIGNLK
                     ESASGSILFEETEAHVLSNDDIEEVLTGVEGLISNGVNQLLVFYYPRDWRLGELVWTP
                     DCDRVEHVRHKYLSASDAFCGDVDRLRRRLAECEICMMFLLIEQPFDHLMAYQHIKPS
                     SFITSNGIDKLDGLQRFAARNGIDPADWLGAGDSPMDNFLRDVGLAVHVGATPLEFDG
                     QRKTVKVRDSLEFGDVLAALSQLP"
     gene            complement(5534..6019)
                     /locus_tag="VF226_20360"
     CDS             complement(5534..6019)
                     /locus_tag="VF226_20360"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7076397.1"
                     /translation="MSLQSSPKDLWSLVREIPVCMLTTRDGEMLRSRPMATRLDEDRQ
                     EFLFLTRSSSPKAHEIGERTQVNLSFSDPEKDLFISVSGEGRLAPDHDIARQLWNSYA
                     AAYFPEGPDSDDVAVLHVSPKKAEYWVGGKPQQVECEELRKAADTGTQPDLGLNEKVE
                     L"
     gene            complement(6259..6459)
                     /locus_tag="VF226_20365"
     CDS             complement(6259..6459)
                     /locus_tag="VF226_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020810978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX7076398.1"
                     /translation="MDWDRIAGNWKQMTGKLKEQWGKLTDDDLQQIAGNREQLEGKIQ
                     ERYGYTKDQARQEIDNWFNRQA"
     gene            6859..8238
                     /locus_tag="VF226_20370"
     CDS             6859..8238
                     /locus_tag="VF226_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEX7076399.1"
                     /translation="MTIPAALDAADGNDHWRVFFASAVFTVFIGGLMVLATMSSEPFR
                     FGIKEGFVLTTFSWVATAAFAAVPFIGVGLSYTDAFFESMSGLTTTGATVIEGLDHLP
                     RGLLLWRSLLQGIGGLGIVVTAIIILPFLRVGGMQLFQAERPDRSEKVLPRAAELVTA
                     AAAIYLALLLACTIAYTVFGMTLFDAVCHALTTVSTAGFSTHDQSFEFFAAPSLEVVA
                     IVFMILGALPFVVFIRMMHGDLKAPWKDAQVRGLLTFLAVVILLMAVWLSSSRNFSFI
                     EALRLTAFNVTSIVTTTGFIITDYTQWGTFAFGMYFLLMFVGGCSGSTAGSIKIYRLQ
                     VAGMLTRSYLLHLISPNRVVSLVYNGRRLPEDVPFSVIAFLAIYMATIGAFTVVLAAF
                     GLEFVTALSAATSAIGNIGPGLGELVGPSGSYAGLPALAKWALCMAMLLGRLELFTVL
                     VLFRPEFWR"
     gene            complement(8266..9450)
                     /locus_tag="VF226_20375"
     CDS             complement(8266..9450)
                     /locus_tag="VF226_20375"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7076400.1"
                     /translation="MSGDRPILIAGAGIGGLSLAIALARQGLPSRVLERRGELAEAGA
                     GIQLGPNAVSALSRLGVADRLKPSIGAPDCLRVHDGRSGQCLAELPLGDWIAKRHQAP
                     YWVAHRADLHAALLETASALPQIELVTASEVTSFTPADGGVVAATADGGRHHGVLLVG
                     ADGIWSRIRKELWPDFSLTYSGKTASRAVVSRADAPAAFRDMATGVWLSPVGHVVHYP
                     VRGGQEIALVVILKEEWPGEGWGLPVDRDRLIGRLGAFSDRLTAFLSSAREWRRWPLY
                     DPEPLPRWSKGRVTLLGDAAHPVLPFLAQGGALAIEDAETLAAAIRMWPSQPAMAIDA
                     YERTRRPRAIRMQAASRRNGVIYHLSNPASLARDLTLRAVPGQRLMALYDWVYGWRPE
                     TL"
     gene            complement(9466..9714)
                     /locus_tag="VF226_20380"
     CDS             complement(9466..9714)
                     /locus_tag="VF226_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX7076401.1"
                     /translation="MADRIVPHFANDKGVEKIFIGVKEFNCMGARPPFDHPHVFLDMG
                     ADHQVICPYCSTLYVYDPRIRADDSDPPGCLVPSAPTV"
     gene            9830..10597
                     /locus_tag="VF226_20385"
     CDS             9830..10597
                     /locus_tag="VF226_20385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7076402.1"
                     /translation="MSEGAVQFFQSDGVEIAYLDEGAGDPILLIHGFASNTNVNWVDT
                     GWVRFLTSNGFRVISIDNRGHGQSQKLYDVEDYGAPLMAEDARRLLDHLGIGRADVMG
                     YSMGARIAAFLALAHPDRVRSCIFGGLGINMVRGVAGTGPIAHALEADSIDQVTNPTA
                     RTFRAFAEQTKSDLKALAACIRASRSPLTAEDVGRISCPVLIAVGSDDVVGGSAEALA
                     DLIPGAEALVIQGRDHMKAVGDRRYKEGVLAFLKRRR"
     gene            10665..11486
                     /locus_tag="VF226_20390"
     CDS             10665..11486
                     /locus_tag="VF226_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX7076403.1"
                     /translation="MLCLSRIGAVCAALLIALPAGPAARADTNVDLELVLAVDVSLSM
                     DYEEQRLQRDGYVAALRDPQVLSAIRSGLHGKIALTYIEWAGAFSQATVLSWTVIDGQ
                     ESAEEVAQKLEQAPISRLRMTSISSALSYAGQQLDASPYRGIRRVIDISGDGPNNSGG
                     PVKPVRDGLVENGVIINGLPIMLRPSQSSIFDISYLDRYYADCVIGGPGAFMIPIRDK
                     SEFATATRQKLILEISGYEPPPQLIPAQFTSDDEATDCTIGEQLWRRYMDGQYRN"
     gene            11726..12445
                     /locus_tag="VF226_20395"
     CDS             11726..12445
                     /locus_tag="VF226_20395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX7076404.1"
                     /translation="MAASVSCSSAVNPTALSFPLMLRTCVIGVLLAAGLLLVWGCSRT
                     PQFIAEPEPWRAEEERACLLSGYVRHSPYLQARSSLGGPSVCGALQPFEMSAAAGGRV
                     SLRPAAMLRCPMITPVDRWVEDTVDPAARRHFGMPVVEVRVAASYGCRPMNNQWGAKL
                     SEHGHANALDVSAFVLADGRTIEVKSGWWGSPRERAFLRAAHNGACAHFTTVLGPDYN
                     SLHRDHFHLDLARRRNGNVCK"
     gene            12515..13018
                     /gene="tsaA"
                     /locus_tag="VF226_20400"
     CDS             12515..13018
                     /gene="tsaA"
                     /locus_tag="VF226_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEX7076405.1"
                     /translation="MGRAVDQDAKRAGELEVPLPDRTDARIVFIGRIRTPFATRDDCP
                     RQGSPDGPVCRIEIDEPYRPGLKGLDRYERVEVLYWLDRARRDLVQQSPKSDGSATGV
                     FALRSPLRPNPIGTSLVTVVGIEPDAILVRGLDCIDGTPLIDIKPDRCLYSPPAASRP
                     GKVVQDA"
     gene            complement(13077..13727)
                     /locus_tag="VF226_20405"
     CDS             complement(13077..13727)
                     /locus_tag="VF226_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076406.1"
                     /translation="MSVSSIAYRLRARAALILACTIALAALLAGCSYGGSGPPAPHYS
                     QFETEAPEGNTVTVCSAHGCRHKSRFTFSRRDIAEIREVMNKAGGSSSPAAERRAIRK
                     AIAWIENRVGPETGTAHDRASLDFLGSGDPGQMDCVDEATNTTSYLTVLAANGLIRHH
                     EVLRPMAKDAPLLRWTHYFAVIRDKHSGQRWAVDSFMRPNGHEPIIMEAEKWYIQT"
     gene            13963..14157
                     /locus_tag="VF226_20410"
     CDS             13963..14157
                     /locus_tag="VF226_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076407.1"
                     /translation="MVLLNLGVEAETSVEAIKNNRKQGVDMWATFLSELREIALLVSI
                     VGSLLAVSVGLAAALAVIVA"
     gene            complement(14184..14744)
                     /locus_tag="VF226_20415"
     CDS             complement(14184..14744)
                     /locus_tag="VF226_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX7076408.1"
                     /translation="MRIFRHLRALALSLMAVLAVVGTTPNANAASAAEIDAAVQDTLE
                     ELFYQVRGARALVNKSAAVLVFPTVYKAGFGIGGEYGEGALITRRRTIDYYNLISASF
                     GFQLGAQARSVVIVFTTPEALESFRRVDGWKVGVDGSVALINVGVGASIDTTRIRSPI
                     VGFIFDNKGLMYNLTLEGSKISRIYR"
     gene            complement(14934..15686)
                     /locus_tag="VF226_20420"
     CDS             complement(14934..15686)
                     /locus_tag="VF226_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076409.1"
                     /translation="MSETVDFTEGGYRYIPGVFQYSAGVAALPGFELVHARFLKPVPL
                     AEGFKRIAGHLASVGRPLTAFCACELRSPAPFTEAGFRAFNETYVGTLAEWGIFKDGK
                     NPVARSNVCPEIAPPSEPSLYAFAYTIPAPNPKPTAVIAGSGEVPEGQSNYRDHVVRP
                     GDTSPEGLAEKARFVLAEMERRLGAVGMTWADVTATQVYTVHDLYPFLADEIVRRGAA
                     THGLVWHLCRPPVVDLEYEMDCRSVRRELVID"
     gene            complement(15700..16728)
                     /locus_tag="VF226_20425"
     CDS             complement(15700..16728)
                     /locus_tag="VF226_20425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076410.1"
                     /translation="MTKPRFHPGYAIGLAGLIALPFVVTALFPSSEKYYLHLAIQVLL
                     WAFIYTGWSLMGRFGLTSLGHGAFTGVGAYVTVLLWNYAGLTPLIGIPLAVLLAAALG
                     LIVGYPCFRQRITGHYFALLTLALAEFVRLCIIGLRDLTGGSLGTQPQRYGNGISLYA
                     VQFEPDRTLAYFIALAIWVIGLYIWHRVDRSMDRYALEAASQDEDAASSVGIDVTREK
                     LKITALSSAMCAFGGAMFGQYQMYIGPDTIAGLGVSLNIVFAAIAGGVYVMLGPTFGA
                     FFTQMLAETLRVSIQGSEALKSWLGSSALALDSAIYGALLVFFIIYMPKGIVGTFLEW
                     VEKRKSGP"
BASE COUNT         3004 a         5303 c         5263 g         3262 t
ORIGIN      
        1 ctggcgaaga tcaacgagca tctgcaaaac atccggccgc tcggagccga cgaggcggcg
       61 cgcgccgtct tccgtgtgct cgacaagaat atcccggccg gggagatgaa cgaggtcaag
      121 cagatgctgc cgccggagat ccggacgctg ttccccggct cggccggtcc ctcggcggca
      181 cagcaacagg tcccgctcga cagataaggt caacttgcgc gagacagcgt cacctcgaag
      241 cctccgacgc gatgctcggt caggacctcg ggcttgaacg caaggccgtg acccggccgc
      301 tcgggaggtc tgagcttgcc gtccaccggg atcgccggct cgatccaaag gtcatcgttc
      361 cagtccatgt attcgagcca gctcgcattt ggaatggacg cgagcaggtg cacgttgagc
      421 tcgtggaaca ggtgcggggc gattgcgatg ccccaggtct cggcgacggc ggcgatcttg
      481 cgcaactcgg tgaggccgcc ccgcatcggg tccggctgca ggatgggcgt gctgggcctg
      541 gtgaagaagg gcctcagatc gtagcgggtg aagtggcttt cgccgccgac gacgcgggtt
      601 ttcagcgccg cagcgacccg ctggtaacct tcgaaatctt cgcagacgac cggctcttcc
      661 agccagtaga cgtcgaggtc gtcgaagtag cggccggcct ggatggccgt atcggcgctc
      721 cagcccatgt tgacgtcgat catgatctcg atgtcgggac cgagtgcctc gcgcatggca
      781 cggacgttcg cgacgtcctc ccgccagggg cggatgtggg caacctgcat cttgattgcc
      841 tgcatcccca tgcccgtgaa gtgcttcgcc ctggcgatca tgccgtctcg gccgaggccg
      901 cgccagcagc ccgagccata ggccggcaca tcatcgcgac acttgcccca gagccggtag
      961 agcggcagcc cggcgcgctt gccgacgatg tcccacagcg cgatgtccac cgcggacagg
     1021 gcaaagacgg aaatccccgc acggcccacc cagtaggtcg atgtccacag cgtctgccag
     1081 atcccttcca cctccgtcgc atcgcggccg agcaccttcg gtgcgatcag ttccttgagg
     1141 caggcatcga tcgtctccag accgccggcg agcggctgca ggtaggccat gcccgtaatt
     1201 ccgccgcggg tggagatctc cagcaccagc agctcccggt gcgtcggcgg catgatgccg
     1261 ttggcgggcg gctcgccggc ccagggaatg cggagaaggg tggtcttgag cgcggcgatg
     1321 gtcgtgtcgg tcacgggcgg cgatcctttt tcggacgatc acagtacatc gtggcacgag
     1381 gcccaggaat cacgcaactt gcatgtcgtt cgatcatcag ggaataatcc ggccggcaac
     1441 tcgcgaatga cctcgccgat caaacccgct gcaacagcgc gcctctagca cgaccgcggt
     1501 ggttctgtgg gttcaatcgc gctgcgatag cccaaggtgg gaattcaagt ctatcggagg
     1561 agaaatgagc ttcgtaaaat ttgctggcct tgccatctcg gcgcttggcc tcgttgcggc
     1621 tggcacggca tcggcacaga cgaccttcaa gatgaacatc tcggtgccgc aggattcgca
     1681 ctacggcgtc gccgtcgata cgttcgcagc cgaggtcgag aagcgcacca acggccgcta
     1741 caagatccag aatttctatg ccagcgcgct cggtaacgag cgtgaggcga tcgaggccgt
     1801 tcagctcggc acgctcgata tggccatgag ctcgacgggg ccggtgccga acttcgtgcc
     1861 ggagctggcg atcctggaca ttccattctt gtttcgggat tacgctcacg cccgggcgac
     1921 gctcgacggt ccgatcgggc aggaaatgct gaagaagttc gagccgatcg gcatcaaggc
     1981 tctggcctgg ggggagaacg gcttccggca catgacgaac tctcagcgtc ccgtgcgcac
     2041 cccagaggac ctcaagggcc tcaagatgcg cacgatggag aaccccatcc acatgcaggc
     2101 ctacaagggc ttcggcatca tcccgacgcc aatggccatc agcgaggtgt tcaccgctct
     2161 gcagcagggc acggttgacg gacaggagaa tccgatctcg gtcatccagt cctggaaatt
     2221 cgaccaagtc cagaagcatt tgacgctgac cggtcacgtc tattccccgg ccttcctgat
     2281 gatgaacaag ggcctctggg acggcctgtc cgatgaggac aagcagcact tccttgacgc
     2341 agccaaggaa gccgtgaagg ccaatcgtgc ccgcatcgat caggatgaga agaacggcgt
     2401 cgcgtatctg cgttcgaagg gcatggaagt gatcgacgac gtcgatcgca cccaattcca
     2461 gaaggccctt gagccggtat ttgcggagct tgccaagaag ttcggcgagg aaaacatccg
     2521 tcgtcttcgt gagggcaagt gatctcataa gcgaatggcg aatagcgaat ggcgaataga
     2581 cggcatgaaa cttggccggt tcactattcg ccagttcgcc attcgctagc ctaggacctc
     2641 agccgatgag agacatcatc ctcaccatcg agcggcacac gacgggcttt gcgatgtggt
     2701 gcgccggcct gatgctggtg atcgcgtcgc tcgccggctt ctaccagatc atttcccggt
     2761 tcgtgctcga gcagccggcg gagtggtccg aggtgaccgt tcgcttttcg ctgatctgga
     2821 tggtgtttct cggcattccg acggcatttc gccagggggc gatgctgtcc gtcgatctgc
     2881 tttatcgaaa gtcgggaccg cgtctcaggc gcgctctgga ctgggtcatt ttcctagcca
     2941 gcctggtcct catgctcgcc attctggggt ggggttacga ctacacgtat cgcacccgct
     3001 tccagaccat tccgggcctg gagttcctca cgatggtctg ggcctattcg gccatgccgg
     3061 tgggcgctgc cttctccttg ctcgcgatcc tagggcagct tctcgatccg cgccatcgcg
     3121 agcttgaaac cgcacagtaa tgtcccgatt ccgaagctcc tagtgtggtt caggtcgaga
     3181 acttcgttct aaggccagtt tctcgatcat cacaccagcg acacctttca accgatcaca
     3241 tcatcaaggg cgctacctca tgtcgatcgc gatgctcata accatgtgcc tgggcttcgc
     3301 gctgtcgatt tcaatcgcgg tgtcgatcgg attggccgcg ctgctcggaa cgtggctgtc
     3361 gaacgtcaac atgctgctgt cggtgaagga gatctatacc gccatcaaca agtttccctt
     3421 ggcggcgatc ccctttttca tcctcgccgg caacatcatg gagagcgggg gcatctccag
     3481 gcgcctcgtc gaatttgcta agtccgtggt cggcggtgtt cagggaggct tgtcggccac
     3541 ctgcgtcctg acctgcatga tcttcgccgc ggtgtccggc tcaagtgtgg cgaccacgtt
     3601 tgccattgga tcaattctcg ttccggcatt ggttcgccac ggctatcccg tcaactacgc
     3661 ggccgcgttg caggcaacct ccgcggagct cggcgtcgtc atcccaccgt cgatcccgat
     3721 gatcatcttc ggtgtatcgg ccgaggtctc gatcggtgaa ttgttcatag ccggaatcgg
     3781 tccgggcatt ctcatcgccg gcgggttgat atcgtttgtt ctgatctatg ctcttatcaa
     3841 aggatacggc aagcaggatc gcgagggcag gcttccgctc ttgaccgcga cgcgccaggc
     3901 gatgtttgcc ctgttcatgc cggtgatcgt tctcggcggc atctacggcg gcgtgttcac
     3961 gccgactgaa gcctccgttg ctgctgtttt ctatgcgctc ctcgttggca tggtcgttta
     4021 tcgagaaatt ggattgaagg atctggggct gattctgcgg aagtccgtca tctcttcgtc
     4081 ggttatcctg ttcatcatcg ccaacgccgg cctgttcagc tacctgatca cccgggccgg
     4141 tgtgcccgat cagatcggcg agtttttgcg cgcgaatctc ggaacgccat tcatgttcct
     4201 gctcggcgtc aacacggctc tcttcttcat cggcatgttc atcgagacgt cggccgccat
     4261 tatcgtgctg gctccaattc tcgtgccggt cgcggcaggg ttcggcatag atccggtgca
     4321 ccttggggtc atcgtcgtcg tcaatctcgc gcttggcatg atcacgccgc cattcggggt
     4381 gaatcttttt gccgcctgcg ccgtagcaaa gatttcggtc gatcagatca tcttgagact
     4441 tgtcccattc gtgctcgtgg tgctcgtctg cctgatgatc atcacttaca tcccgcccat
     4501 ttcactcttc cttcgggatc ttatgtatta ggcggaacga tcgatccggg ctgcggttgt
     4561 ccggctgtcg attgcctggg aagaccattg acggatggac aatcaggctt ggatggaagc
     4621 gctgcgggat ttcgcggcga cctcacggtt tcgcgagggc ggcggcatcg tcacggacct
     4681 cgacgggacg gcggtgcatg agcacgaagg ccgcatcgtc attcctcaca atgtctcgca
     4741 cgcgctgcaa cgcttgaacg agcagggcca cccgatcgcg atcaacacgc tgcgcttccc
     4801 gatgagcgtc atcggcacct tcggccagga atggtacgcg atcaccaacg cgccgctccc
     4861 gctcatctca ctcaacggca gcttgatcgg aaacctaaag gaaagcgcca gcgggtccat
     4921 cctcttcgag gaaaccgaag cgcatgtgct gtcgaatgac gatatcgaag aagtcctgac
     4981 cggggtcgaa gggctgatca gcaacggcgt gaaccagctc ctcgtcttct actatccgcg
     5041 tgactggcgg ctgggggaat tggtgtggac gccggactgc gatcgcgtcg agcatgtcag
     5101 gcacaagtac ctgagcgcct ctgacgcgtt ctgcggggat gtcgatcggc tgcggcggcg
     5161 gctcgcggaa tgtgaaatct gcatgatgtt cctgctcatc gagcagccgt tcgatcacct
     5221 gatggcctat cagcacatca agcctagcag cttcatcacc agcaacggga tagacaagct
     5281 cgacggcctt cagcgcttcg cagcgcgcaa cgggatcgac ccggccgatt ggctcggcgc
     5341 cggcgattcg ccgatggaca atttcctgcg ggatgtcggg ctcgccgtgc acgtcggggc
     5401 gacgccactc gagttcgacg gccagcgaaa aaccgtgaag gtccgagact cgctggagtt
     5461 tggagacgtc ctcgcggcgc tgtcacagct cccctaggtt cgcggccggg aatgctccga
     5521 gaccccgatg gcgtcaaagc tctactttct cgttgaggcc gagatcgggc tgggttccgg
     5581 tgtccgccgc cttgcgaagc tcttcgcatt ccacttgctg tggcttgccg cccacccagt
     5641 attctgcctt ctttggcgag acgtgcagca cggcgacgtc gtcgctgtcc gggccttccg
     5701 ggaaataggc cgcggcatag gagttccaga gttggcgtgc aatgtcgtga tccggggcga
     5761 gccggccctc accggacacc gatatgaaca ggtccttctc cgggtcggaa aacgacagat
     5821 tcacctgggt ccgctccccg atctcatggg ccttcgggga tgacgacctg gtcagaaaca
     5881 ggaactcctg cctgtcctcg tcgaggcgcg tcgccatggg gcgggagcgc agcatttcgc
     5941 cgtcccgcgt cgtgagcatg cagacgggga tctcgcgcac cagcgaccag aggtccttcg
     6001 ggctggattg cagggacatg ctggggtcct ccgctgctgt cgattgcttt cagggcaaac
     6061 tttcggcaaa gcctgcgagt tccgcgattg cgctgatcgg ccatgccgtc acgcgagccc
     6121 cagcacgttg atgatgaaaa gaatgatgac gacgaggccg acgagataga tgatggtgtt
     6181 catgagggtc ctctcaatgt tggcctgccg gcagccaagg agctggccgc cggcagctgc
     6241 aaggggtggc ggggaccctc aggcctggcg attgaaccag ttgtcgatct cctggcgcgc
     6301 ctgatctttg gtgtatccgt agcgctcctg gattttgcct tcgagctgct cacggttccc
     6361 ggcaatctgc tgaaggtcgt cgtcggtgag cttgccccat tgctccttga gcttgccggt
     6421 catctgcttc cagttaccgg cgatgcgatc ccaatccatg atgaactccg atctgtgatt
     6481 tctgacttgc aactggcgcc acctggcgac gcctcgctgg gtcaacgcgc tttcatggct
     6541 ggtgttccag cccaagctga atgcttccag gggcgtgctc agaacaaacg atgaacatgg
     6601 ctccgagcga caggctcagg atagagggac cgttgcggtg gcgacctatg cgcgcggaaa
     6661 gaagcagcgc tccgcccgac agggttgctg cattgacttg acgttcagct ttaaagacca
     6721 caatccggcc ggccagctca tgtgccgtgc gggcgcacgg tgcgggcgca atgtggcact
     6781 cacagcgggg cgggcttgct tcagcttcgg ccaatcatct tcacgttggg cctgctgctc
     6841 tgtgcggtag cggccgcaat gaccattcct gccgctctcg acgcggcgga cgggaatgat
     6901 cattggcggg tcttctttgc ctccgcggtg ttcacggtct tcatcggcgg cctgatggtc
     6961 ctcgccacga tgtcgagcga gcccttcagg ttcggcatca aggaggggtt cgtcctcacc
     7021 acgttcagct gggtcgcgac ggccgcgttc gcggccgtgc cattcatcgg cgtcgggctg
     7081 agttatacgg atgccttctt cgaatcgatg tcaggcttga ccacgaccgg cgccacggtg
     7141 atcgaagggc tcgaccacct gccgcgcggc cttctgcttt ggcgctcgtt gctacaagga
     7201 attggcggcc tggggatcgt cgtcacggcc atcatcattc tgccgttcct gcgcgtcggc
     7261 gggatgcagc tcttccaggc cgagcgtccg gaccgttccg aaaaggtcct gccgcgtgcc
     7321 gccgagctcg taacggccgc tgcggccatc tacctggcgc tgctgttggc ctgcacgatt
     7381 gcttacactg tgttcggcat gacgctcttc gacgccgtct gccatgccct gacgaccgtc
     7441 tcgacggccg gcttttcgac ccacgaccag agcttcgaat tctttgctgc gccgtcgctc
     7501 gaggtggtcg cgatcgtatt catgatcctc ggcgcgttgc cgttcgtcgt gttcatcagg
     7561 atgatgcacg gcgacctcaa agcgccttgg aaggacgctc aggtccgggg cctgctgacg
     7621 ttcctggcgg tcgtgatcct gctcatggcc gtgtggctgt cgtcgtcgcg gaatttcagc
     7681 ttcatcgagg cactgcgcct gacagccttc aacgtaacct cgatcgttac gaccactggc
     7741 tttatcatta ccgactacac ccagtggggc accttcgcgt tcgggatgta cttcctattg
     7801 atgttcgtcg gcggctgttc gggctccact gcgggctcga taaaaatcta tcgtctgcag
     7861 gttgccggca tgctgaccag atcctacctg ctgcacctca tcagcccgaa ccgggtcgtc
     7921 agcctcgtct acaacggccg ccgtcttccc gaggacgtgc ccttttcggt cattgcattc
     7981 ctcgcaatct acatggcgac catcggcgcg ttcacggtcg tgctggcggc ctttggtctc
     8041 gagttcgtga ccgcgctgtc ggcggcaacc agcgccatcg gcaacatcgg accgggcctc
     8101 ggagagcttg tcggcccctc gggcagctat gccggcctgc cggcactggc gaaatgggcg
     8161 ctgtgcatgg ccatgctgct cggacgattg gagctgttca cggttcttgt gctgttccgt
     8221 ccagagttct ggcggtagcg ggggaatctt cggcgcgact tggaatcaga gtgtctccgg
     8281 ccgccagccg taaacccaat cgtagagcgc catcagccgt tgccccggca cagcgcgcag
     8341 cgtcagatcg cgcgcgaggg acgcagggtt ggagaggtgg taaatcacgc cgttgcgtcg
     8401 cgaagcagcc tgcatgcgga tggcgcgggg gcggcgtgtg cgttcatagg catcaatggc
     8461 catggccggt tgggagggcc acatcctgat ggcggccgcc aacgtctccg catcctctat
     8521 ggccagcgca ccgccttggg cgagaaacgg cagcacgggg tgggccgcat ctccgaggag
     8581 cgtaacgcgt cccttcgacc aacgcggcag cggctcggga tcgtacagcg gccagcgccg
     8641 ccactcgcgg gcagatgaca ggaacgcggt gagcctatcg gaaaatgcgc cgagcctgcc
     8701 gatcaggcgg tcccgatcga cgggcaggcc ccatccttcg ccgggccatt cctccttcag
     8761 aatgacgacc agcgcgatct cctgcccgcc gcgaaccggg tagtggacga catgcccgac
     8821 aggtgagagc cagacgccgg tcgccatgtc ccgaaacgcg gcaggggcgt cggcccggct
     8881 tacgacggca cgcgaggcgg tcttgccgga gtaggtgagg ctgaaatccg gccagagctc
     8941 cttgcggatt cgggaccaga tcccgtccgc tccgacaagc aggacgccgt gatgcctgcc
     9001 gccatcggcc gtggctgcaa cgacgccgcc gtccgccggc gtgaaagacg tgacttcaga
     9061 cgcggtgacg agctcgatct gaggaagtgc cgaggcggtt tccaatagcg cagcatgcaa
     9121 atcggcgcga tgtgcaaccc aatagggggc ttgatgacgt ttggcgatcc agtcgccgag
     9181 cggcagctcg gcgaggcatt gacccgatcg gccgtcatgc acccgcaggc aatcgggcgc
     9241 tccaatagac ggcttcagcc gatcggcgac acccaaccgg gagagggcgc tgacggcatt
     9301 gggtccgagc tgtatgccgg cgccggcctc cgcaagctca cctcgtcgct cgagcacccg
     9361 gctgggcaat ccttgccggg ccagggcaat ggcaagggaa aggccgccga tgccggctcc
     9421 ggcgatcaga atgggccgat cgcctgacac gtgaagtgtc cggtctcaga cggtcggcgc
     9481 tgacggcaca aggcagcccg gcgggtcgga atcatcggct ctgatacgtg gatcgtagac
     9541 gtaaagcgtc gagcagtacg ggcagatcac ctgatgatcc gcgcccatgt cgaggaagac
     9601 atgcgggtga tcgaagggcg ggcgcgcgcc catgcaattg aactccttga cgccgatgaa
     9661 aatcttctcg acgcccttgt cgttcgcgaa atgggggacg attctgtcgg ccatccggct
     9721 tgcccttgca cctctggtta ccagctagcg tgatgaactt ggaactttgt cgggaccata
     9781 cccgtccgac cggcgaaatg atagggaagc ctctgtcgca atcgacggaa tgagcgaggg
     9841 tgccgtgcag ttcttccaat cggatggtgt cgagatcgct tacctcgacg aaggagcagg
     9901 ggatccgatc ctcctcatcc atggcttcgc ctccaacacc aacgtgaact gggtcgacac
     9961 cggctgggtg cggttcctga cctccaacgg cttccgcgtc atctcgatcg acaaccgggg
    10021 acatgggcaa agccagaagc tctacgatgt cgaggactac ggcgcacccc tgatggccga
    10081 ggacgcccgc cggctcctcg atcatctcgg cattgggcgt gcagacgtca tgggctactc
    10141 gatgggtgcg cgtattgctg cctttctggc gctggcccat cccgaccggg tcagaagctg
    10201 catcttcggc gggcttggaa tcaacatggt ccgcggcgtg gccggcaccg gccccatcgc
    10261 ccatgcgctg gaggccgata gcatcgatca ggttacgaac ccgacggcgc ggacattccg
    10321 cgccttcgcc gagcagacga aaagcgacct gaaggcgctc gcagcgtgca tcagagcctc
    10381 gcgatccccg ctcacggcgg aagatgtcgg ccgcatctcg tgtcctgttt tgatcgccgt
    10441 cggcagcgac gacgtggtgg gcgggtcggc ggaggcgctc gccgacctca ttcccggtgc
    10501 cgaggctctc gtcattcagg gtcgcgatca catgaaggcg gtcggcgatc ggcgctacaa
    10561 ggagggcgtg ctcgccttcc tgaagcggcg acgctgaagc tcacattccg tttacctgcc
    10621 gcgccgccat tgcaactttt ccgctgtgcg acgatttatt tcgtatgctg tgcctttcgc
    10681 gtatcggcgc agtctgcgcc gcactcctca tcgcgttgcc ggctggacct gccgcgcgcg
    10741 ccgacaccaa tgtcgacctc gagctcgtgc tcgctgtcga tgtttccctt tccatggatt
    10801 acgaggagca gcgcctccag agggatggct acgttgcggc tttgcgggac ccgcaagtcc
    10861 tctcggccat caggtcgggg ctccacggaa agattgcgct tacttacatc gagtgggcag
    10921 gagcgttttc ccaggccacg gttctctcct ggaccgtcat cgatgggcag gagagcgcgg
    10981 aggaagtggc gcagaagctg gagcaggcgc ccatctcccg gctccgcatg acgtcgatct
    11041 cgtcggccct ttcctatgca ggccagcagc tcgatgccag tccctatcgc ggcatccgcc
    11101 gcgtcatcga catctcgggc gacgggccca acaactcggg cggcccggtc aagcccgtgc
    11161 gtgatggcct ggtcgaaaac ggggtcatca tcaacgggtt gccgatcatg ctgcgtccca
    11221 gtcagtcgag cattttcgat atcagctatc tcgaccgcta ctacgccgac tgtgtcatcg
    11281 gtgggccggg cgccttcatg atcccgatcc gcgacaaatc cgagttcgcg accgccacgc
    11341 ggcagaagct cattctggaa atctccggct acgagccgcc gcctcagctg atcccggcgc
    11401 agttcaccag cgacgacgag gcgaccgact gcacgatcgg cgagcagctc tggcgccgct
    11461 acatggacgg gcagtaccgg aattgaagcg ccttccgatg ccatggatga cggacggtca
    11521 cttgctgacc gccccgttcc aacggcggca ggtgttcaac ctcatccggc ccttcgcgcc
    11581 gatctccctc catggcgagg tgaggaaagt gcctgctccg tcgcttccag cttcgtctga
    11641 aattttcaag gccaagcctc gcccaaatcc gccgctctgg cgccccgacc acgtctgatt
    11701 tgcgccgcga aagcagccaa cggcaatggc agcttccgtc agctgctcat ctgccgttaa
    11761 ccctaccgca cttagctttc cgctcatgtt gcgtacctgc gtcattggtg tgctgctcgc
    11821 agccggactt ctgctggtct gggggtgcag ccgcacgcct caattcattg ctgagccaga
    11881 gccgtggcgg gcggaggagg agcgtgcgtg cctgctctcc ggctacgtgc ggcactcgcc
    11941 ctatctgcag gcccgctcct cgctcggcgg gccaagcgtc tgcggcgcgc tgcaaccgtt
    12001 cgagatgtcg gcggctgccg gcgggcgtgt cagcttgcgg ccggcggcga tgctgcggtg
    12061 tcccatgatc acgcccgtgg accgctgggt cgaagacact gtcgatcccg ctgcccgccg
    12121 ccacttcggc atgcccgtcg tagaggtgag agtggccgca tcctacggct gccgccccat
    12181 gaacaatcag tggggggcca agctctcgga gcacggccac gctaacgcgc tcgacgtctc
    12241 tgcgttcgtc ctcgccgatg gacggaccat cgaggtgaaa tcgggctggt ggggcagccc
    12301 tcgtgagcgc gccttcctcc gggcagctca taacggcgcc tgcgcccact tcacgaccgt
    12361 gctcggtccc gactacaaca gcctccaccg ggatcatttt cacctcgatc tggcgcgccg
    12421 gcgaaatggc aacgtctgca agtgagggcg ggcgcggatt cgcctgttgc ctcgtggcgt
    12481 ctgtcagatc atcttgggtt gcgagcgaaa ctggatgggg cgagccgtgg atcaggatgc
    12541 aaaacgcgcg ggcgagctgg aagtcccgct gccggaccgc accgatgcac gcatcgtctt
    12601 catcgggcgc atccgcacac ctttcgcgac gcgggacgac tgcccgcggc agggctcacc
    12661 tgacgggccg gtctgccgca tcgagatcga cgagccgtat cgtccagggc tcaaggggct
    12721 cgatcgctac gagcgggtcg aggtgcttta ctggctcgat cgcgcccgcc gcgacctggt
    12781 gcagcaaagc ccgaagtccg acgggtcggc aacgggtgtc ttcgcgctcc gctcgccgct
    12841 ccgcccaaat ccgatcggga catcgctggt gaccgtggtc ggcatcgagc ctgatgcgat
    12901 cctggtccgc ggcctcgact gtatcgacgg cacgccgctg atcgacatca agcccgatcg
    12961 ctgcctctat tcgccgcccg cagcaagccg ccctggcaag gttgtccaag acgcctgacc
    13021 tatggaggcg cgacctcaac tggcgcaggc tttacgcacc aacggcagcg ttttcgtcag
    13081 gtctggatgt accacttctc cgcttccatg atgatcggct cgtggccgtt cgggcgcatg
    13141 aagctgtcga cagcccaccg ttggccggaa tgcttgtcac ggatcaccgc gaagtagtgg
    13201 gtccagcgta gaagcggggc atccttggcc atgggacgca aaacctcgtg gtgccggatg
    13261 agcccgttcg ccgcgagtac cgtgaggtag ctggtcgtat tcgtcgcctc gtcaacgcaa
    13321 tccatctggc ccggatcgcc actcccaaga aaatcaaggc tggcgcggtc atgcgccgta
    13381 cctgtctcgg ggccgacccg gttctcgatc cacgcgatcg ccttgcggat cgcgcggcgc
    13441 tcagccgctg gactggagct gccaccagcc ttattcatga cctctctgat ttcagcgata
    13501 tccctgcggc tgaacgtgaa ccgtgacttg tgccggcagc cgtgcgcgct gcatacggtg
    13561 accgtgtttc cttccggcgc ttcggtttcg aactgagaat agtgaggcgc cggcggcccc
    13621 gagccgccgt agctgcagcc cgccagaaga gcggccagtg caatagtgca ggcaagaatg
    13681 agagccgctc gcgcgcgcaa gcggtacgca atactggaaa ccgacatgga tactcgtgga
    13741 cactgaccgc cccccgctgg acggcgagca tcccagccgt tctgggcccg agcacggctg
    13801 actctaggcg ttgttggagc aacttatggc gcacgtgcgg caccggccgg ggtcggatca
    13861 cctgggcgca gcgatttgtc gaggtcggac aacagtgccg ccgaaactta ccgtcagttc
    13921 agcacacaat gcattttacg gaactatttc gaggaatgtt cattggtact gttgaacctt
    13981 ggcgtcgagg ctgagacttc ggtcgaggct atcaagaata acaggaaaca gggcgtagac
    14041 atgtgggcca cattcctcag cgagctgcgc gagatcgctc ttctggtatc gatcgtgggc
    14101 agccttctgg cagtcagcgt cggactggct gccgccctgg ccgtcatcgt cgcctgaccg
    14161 atgaactcgg gccacttgcc cgctcaacga tagatgcgac tgatcttgga gccttccagc
    14221 gtcaggttat acatcaggcc cttgttgtcg aagatgaacc cgacgatggg gctgcggatg
    14281 cgcgtcgtat cgatggaggc accgacgccg acattgatca gggcgacgga tccgtcgacc
    14341 ccaactttcc agccatccac ccgccggaag ctctccagcg cctcgggcgt cgtgaacacg
    14401 atgacgacgg agcgagcctg cgcgccaagc tggaaaccga acgatgctga aatgagattg
    14461 tagtaatcga tggtcctgcg gcgcgtgatc aacgcgcctt cgccatactc cccaccaatt
    14521 ccgaagccgg ccttgtaaac ggtcgggaat accagaaccg ccgcggactt gttgaccagc
    14581 gcgcgtgctc cccggacctg ataaaagagt tcttcgagcg tatcctggac ggcagcgtcg
    14641 atttccgctg ccgaagcggc attcgcgttc ggagtcgtgc cgaccacggc cagcactgcc
    14701 atcaacgaga gcgccagagc cctcaggtgc cgaaaaatcc gcatgttttt acccttccga
    14761 atgcgagctc cctcagcccc gactcgccac acagggaccg agatagcacc ttccggcggc
    14821 cacattctgg cctggttgca tcttctgcgt gcaccgttgt gcatcggcca cggttgcggc
    14881 ctgccgcgca gatgccggga tcgccatccg gcgagcgcca gcattctcgg atctcagtcg
    14941 atcaccagct cgcggcggac agagcggcag tccatctcgt actcgagatc gacgaccggc
    15001 gggcggcata ggtgccacac gaggccgtgg gtggccgctc ctcgccggac gatctcgtcg
    15061 gcgaggaacg gataaaggtc gtgcacggtg tagacttggg tcgccgtgac gtcggcccag
    15121 gtcatgccga ccgccccgag gcgccgctcc atttcggcca gcacgaaacg cgccttttcg
    15181 gcaagacctt ccggtgaggt atcgccgggc ctcacgacgt gatcccggta attggactgg
    15241 ccctcgggaa cctcgccgct gccggcgatc actgccgtcg gtttggggtt cggcgccggt
    15301 attgtgtagg cgaacgcata aagagacggc tcgctaggcg gtgcgatctc ggggcagacg
    15361 ttcgaccgcg cgaccgggtt cttgccatcc ttgaagattc cccactcggc cagcgtgccc
    15421 acataggtct cgttgaacgc gcgaaacccc gcctctgtga agggcgcggg cgatcgcagc
    15481 tcgcaggcgc agaaggcggt gagcgggcgg ccgacggaag caaggtgccc ggcaatgcgc
    15541 ttgaagcctt ccgccagcgg aacgggtttc aagaaccggg catgcacgag ctcgaagccg
    15601 ggcagggcgg caacaccagc cgaatactgg aagacgccgg gaatgtagcg atacccacct
    15661 tcggtgaaat cgaccgtttc gctcaatgcg tatcctccgt catgggcctg atttgcgctt
    15721 ctcgacccac tcgagaaacg tgccgacgat tcctttcggc atatagatga tgaagaacac
    15781 gagcagcgcg ccgtagatgg ccgaatcgag cgcgagcgca ctactgccga gccagctctt
    15841 cagggcctcc gagccctgga tcgagacgcg caaggtctcg gccagcatct gcgtgaagaa
    15901 ggcgccgaat gtcgggccga gcatcacgta tacgccgccg gcaatggctg cgaacacgat
    15961 gttgagcgac acgccaagtc cggcgatcgt gtccggcccg atgtacatct gatactggcc
    16021 gaacatcgcc ccgccgaacg cgcacatcgc cgacgacagc gccgtgatct tgagcttttc
    16081 ccgcgtcacg tcgatgccga cggacgaggc cgcgtcctcg tcctggctgg cggcttcgag
    16141 tgcgtagcgg tccatgctgc ggtcgacccg atgccagatg taaaggccga tcacccagat
    16201 cgcgagcgcg atgaaatatg caagcgttcg gtcgggctcg aactgcacgg cgtaaagcga
    16261 aatgccgttc ccgtagcgtt gcggctgcgt gccgagtgag ccgcccgtca gatcgcgcag
    16321 cccgatgatg cacagccgca cgaactcggc gagcgccagc gtcagcaagg cgaagtagtg
    16381 gccggtgatc cgctgccgaa agcacggata gccgacgatc agcccgagtg cggctgcgag
    16441 caggacggcc agcgggattc cgatgagcgg cgtcaggccg gcgtagttcc ataagaggac
    16501 ggtcacgtag gcgccgacgc cggtaaaagc gccgtggccc agcgaggtca gtccgaaccg
    16561 gcccatcagc gaccagccgg tgtaaatgaa cgcccacagc aggacctgga tggcaaggtg
    16621 caggtagtat ttctcagacg acgggaacag cgcggtgacg acgaacggca gagcgatcag
    16681 ccctgcgagc ccgatcgcat atcccggatg gaagcgaggc ttcgtcatgc cttgctcccg
    16741 aacaggccac gcgggcgcag gaacatcatg accatgaaga acaggaaggc gatggcgtag
    16801 ccccattcgg tcgcgaagca ggagccgccg at
//
LOCUS       DASVDU010000305        15853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_86383, whole genome shotgun sequence.
ACCESSION   DASVDU010000305 DASVDU010000000
VERSION     DASVDU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15853
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86383"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF226_20430"
     CDS             <1..502
                     /locus_tag="VF226_20430"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX7076411.1"
                     /translation="TGRLECLKPSQTAELISAIAETSATSAGGGEATERLLLLERKSG
                     KRPFLAEVAPLRDITGELERGFAGAVVFLIDPENAKAISTERLSRYFGLSQAESEVAR
                     AMVDGLSTGEIADLRGTKVETVRSQFKSIYSKTGIRRRADLVRLAVSVDPPIVRSANA
                     TEGASA"
     gene            499..1281
                     /locus_tag="VF226_20435"
     CDS             499..1281
                     /locus_tag="VF226_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7076412.1"
                     /translation="MSSGSGCRVERQGAVLWITIDRPQVLNALDAATHAALADAFDTY
                     AADPELRVAVLTGAGERAFCVGSDLKQRAMTNRDHHPPTGFGGLTHRFDLFKPVICAV
                     NGLALGGGVELMLACDLVIAADHAEFALPEPRVGLTALGGGGLQRLARQLPLKQAMWL
                     ALTGRRINAHEAREMGLVNEVVPQADLKARAMALSGELLDMAPLALEASKQVMLQSLA
                     CPDLATAMNAEYPAAKRMLASEDAVEGQGAFMEKRKPVWKGR"
     gene            complement(1290..1724)
                     /locus_tag="VF226_20440"
     CDS             complement(1290..1724)
                     /locus_tag="VF226_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX7076413.1"
                     /translation="MSEFYVKVGDGATFSKTVGETDVYLFAGITGDLSPNHVNKSYME
                     KSKFGRLQAHGALMVGFMSTASTLAIAHVRDTADATPMSLGYDRVRFLAPVYFGDTVT
                     VDYRISSIDLERQRTTADISIKNQDGVLVAVGTHILKWVKNE"
     gene            1819..2559
                     /locus_tag="VF226_20445"
     CDS             1819..2559
                     /locus_tag="VF226_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076414.1"
                     /translation="MRETLTGQVAIVTGAARGIGLGIAERLAAEGAKVVIWDRDLAPL
                     ERSRKSFSPAAAEIVDVADYASVERAFDAAVRSFGKVDILVNNAGVNGPVAPTWDYPL
                     EEWDRVVAIDMTAVFYCCRVAARHMRERRQGRIITVASIAGKEGMPYIPAYSAAKAGV
                     IGFSKALARELCDVGVTVNCIAPAITETELMREMTPEHIERARAKIPMGRFVKIEEIA
                     AMCAFIASPDCSFTTGFVFDVTGGRATY"
     gene            2659..3483
                     /locus_tag="VF226_20450"
     CDS             2659..3483
                     /locus_tag="VF226_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEX7076415.1"
                     /translation="MLDAGLLSINQATLMQCSFEESIAVLARHGVRATSIWRDKLREY
                     GLSKAQQLLKSEGLAVSGFCSGGLITSPDAREAAEAMDEVRRAIDETAAVEARCLVFV
                     AGGVDPRDKDIKATRARVLDRIAELVPYARAANVTIALEPLHPMTCATRSVLTTVGLA
                     NDWCDVLNAEDVVGIAVDTYAVWWDPNLEAEIARAGKRICAFHINDWLPDTQDLRLDR
                     GMMGDGLIDIPRIRRMVEEAGYKGHCEVEIFSARNWWKRDPDEVVRTILERCQTAV"
     gene            complement(3638..4474)
                     /locus_tag="VF226_20455"
     CDS             complement(3638..4474)
                     /locus_tag="VF226_20455"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX7076416.1"
                     /translation="MNATRAACVIGWPAKHSRSPKMHGWWLKHYGIDGDYRIEEVPPE
                     DFPDFIGNLRGHGYVGANITLPHKEAAFRLTEPDERARAVGASNTLWYDNGVLRSTNT
                     DVEGFIGALDAGAPGWDQSGGHAVVIGAGGAGRAVVWGLLERGVTRIHVVNRTYSRAV
                     DMAERYGDRVIPEHWEDRNKVLEGATLAANATSLGLKGNADLDVDLSRMAPGGVVADI
                     NYVPLETKLLAQARRLGLKTANGLDMLLYQAGTGFELWFGIRPEVTPELRELLARDIP
                     RS"
     gene            complement(4479..5543)
                     /locus_tag="VF226_20460"
     CDS             complement(4479..5543)
                     /locus_tag="VF226_20460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7076417.1"
                     /translation="MAPVRIAVIGSGMIGRRHIEVLKNDPDRHVLAAIADPAPAAAQL
                     AGELGVPHFTSHDILLDREKPDGVIIATPNQLHASVGLACVERKIPVLVEKPIADSIA
                     AALDLVEAAERARVPVLVGHHRRHNPIMQKAAEVIRSGGIGKVLAVAGLWLSHKPKDY
                     FNVSWRGQPGGGPVLINGIHDIDCLRMLCGEIETVQAKTARTARELPVEDTAAAVLTF
                     ASGALGTLLISDSASAPWSWEWTSRENPFYPNEPENCYLISGTRGSLTVPSLELRWHE
                     EGRENWGLPLKMERIPVRPADPYVEQMRNFVDVICGRSEPVLSGADGTRTLAATLAIS
                     ISAESGQPVRVADLLKRERS"
     gene            complement(5553..7082)
                     /locus_tag="VF226_20465"
     CDS             complement(5553..7082)
                     /locus_tag="VF226_20465"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX7076418.1"
                     /translation="MAFNKFTDAATAVGLIKDGDTVALVGGGGGLVEASCLFAAVEKR
                     FLETGHPRDLTVVHALGIGDRKTRGMNCFAHEGMLKRVIGGHWVWSPRMQALARENKI
                     EAYVLPGGVTMQLFREIGAGRPGLFTHVGLGTFVDPRIEGGRMNSRAKDDLVEVVEID
                     GRELLRYRTFPVNIAVIRGSFADAHGNISLDQEAANLDIYAAALAAHNSGGKVIAQVR
                     TAVDVGVLPARSVRVPGAIVDAVVVDPTQSITYDVVHDPTLSGEIKGPLPPAPEPPFS
                     VRQVIARRAADELYDGAVLNYGFGIPDGVAKLVAARGHLDRYYQTIEHGTYGGALLDG
                     TLFGFARNATAMIDGPSQFDFYSGGGLDIAFLGFGEIDASGNVNVSKLAGTTVGPGGF
                     IDIAQNARKVVFCGTFDTKGTELELGGGRLRIARNGDVCKLVKQVEQITFSGAISRRR
                     GQEVVYVTERAVFRLSDDGLVLTEVAPGIDVRRDVLERLEFAPQVPRDPALINPAHFN
                     P"
     gene            complement(7096..8271)
                     /locus_tag="VF226_20470"
     CDS             complement(7096..8271)
                     /locus_tag="VF226_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7076419.1"
                     /translation="MDFALSDEQRMLIETARRFVYDELVPLEEEVEAKRQLDPAIARA
                     IFEKSRALGLYAVNIPAEYGGGGLSAVDTCLAEEQFGHTTDILVRRAFGNVYEVLLVA
                     TEAQRERWLKPAVRGERTFSVAFTEPEAGSDAAGIKTSAVRRGNGWVLNGHKHFISDG
                     LYSDFFVVSAVTDPAAGARGISLFLVDKGMPGFTIGRDQPMMGLGGTSHVEMFFEDVE
                     LGPEHLLGGEGEGLKLALETLGRIRLAHIGARCVGKATRLLRMMTDYARERRQFGKSI
                     GEFQMVQQMLADSAMEINAARLMVLHAASEIDEGRDPRERISMVKAYASETLGRVADR
                     AVQVFGGMGYCKDMPIERLYRDARIFRIFDGTSEIHRMVIARSMLKHGAGRFADVIE"
     gene            complement(8291..9079)
                     /locus_tag="VF226_20475"
     CDS             complement(8291..9079)
                     /locus_tag="VF226_20475"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7076420.1"
                     /translation="MSREFEGKVVVVTGGSRGIGRAIGAAFADRGAQVVLAASSAANL
                     DKAAEAIAERGAKRPITVAADLREQQGCEAVYNKVQAELGRCDVLVNSAGATKAGAFL
                     EQPDEDWHDGFALKFFAAVRLSRLFWPMLKRSQGFVVNIIGGAARIPDPEFLVGGAVN
                     AAFANFSKGLSGIGMRDGVNVNAILPGLTETERVEQLFQQRAVALKTTPDEVRARAMA
                     KNGLRRLGTPEDVAELAVFLASERARHIQGTSIAVDGGATSGVY"
     gene            complement(9090..10040)
                     /locus_tag="VF226_20480"
     CDS             complement(9090..10040)
                     /locus_tag="VF226_20480"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7076421.1"
                     /translation="MPFEQPQIVVAGAGAMGGLFGGLLAEHGLKVTLLDPWAEHVAAI
                     NDRGLRIVGVGGDRTIPVKATTDAASLTEADVVLFQCKSFANEAAAKSVKHLFGRGAV
                     AISFQNGLGNEDVLGAILGAENVLGGLTAQAGLVEAPGVVRNFGNLPTYIGELQGRLS
                     DRAISIANTFTRHGLPTSASADIKREKWKKLLGNVALGAISAVTDLRSCEIMAVPELR
                     EVVFRSVDEAAAVAAAEGYKLDTGEAREILMRLVDTTGGGTGAAKSSMREDIVRKRRT
                     EIDTIHGAVARLGRAHGVPTPTIDAMVALVKGLESQYLNG"
     gene            complement(10083..10883)
                     /locus_tag="VF226_20485"
     CDS             complement(10083..10883)
                     /locus_tag="VF226_20485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076422.1"
                     /translation="MLLETKSLTKSFGGLVAVNNLDLAIREGEVRGIIGPNGSGKSTL
                     FNLISGVYRSDPGSKVVFDGRDITNEEPHVIARLGIARTFQLLRIFGGMTVLENLLIG
                     HHAHIRYAITSAILGTRRVQAEERRVREEMMELLSFIGLADYADMPAAEMSIGQRRLL
                     ALGRAIAMRPKLLMLDEPAAGLSPVNVDKLLETVLALKSRYGLTVIIIEHILKVVMES
                     CETITVLDHGEKIAEGPPAAIKEDHRVIEAYLGKEMGDAEVREFLRAS"
     gene            complement(10873..11904)
                     /locus_tag="VF226_20490"
     CDS             complement(10873..11904)
                     /locus_tag="VF226_20490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076423.1"
                     /translation="MSVENIAEPSVVRPSVFSRLGAMHPIVPWIIGFVVLLPLPLLMP
                     NSYQQYIVNVICINIILAVGLNIVKGFAGQVTVGHIAIAAIGAYSSAVLSTKFGLPFW
                     LAFPSAILIAGLAGAIVGIPSFRLEGAYLALATLGLAESVRIFISATDYLGAAIGYEN
                     IPPPYFFGHALNTHASYFYIVMPLALIGMYFSFSILRSDIGRAFKALREDPLAAAATG
                     IDVRKYKVIAFVISALYAGCAGSLLAHMTPGFIHPNNYTIMEMVTVLLMIVFGGIGHI
                     WGGVVGAILVTIIHDFTRDYYQYQMLMFGGVIVLTVLYMPKGIGGLIDQYLVRRRFNA
                     IRQVKTHAA"
     gene            complement(12173..13387)
                     /locus_tag="VF226_20495"
     CDS             complement(12173..13387)
                     /locus_tag="VF226_20495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076424.1"
                     /translation="MFKAMRHAVPLAALALGVAAVPALAEDNIRIGITLRMVVENGLK
                     YGQMTKDELEAVNASGGINGKKVEVILLDDECKPDKGIANVNRFIHQHKVHLVIGSTC
                     SSVSLPMVDITAKEEVPQIVPHSTNSSITQKGSAWVFRTAVAERFYASVHAKYLSENV
                     GKKVAYLYTTDGAGIGFAKDYMNFMRRTYQVEPVYEAQMQETDLDFRSHLLKIKSLNP
                     DVLAIGGQLEAIARITQQSYEVGIPSRVRRVAASAASNAPVPELAGEAAKGLTFAAAF
                     SCKDERPIAQEFVKMVQEKYKVRCPDHDFSQAYETAQLVKLALSRANLKLTEESLAED
                     RRAIRDALASIKDYHGLASGPINFCADPTPQCRDGNRTGILVEYTKGGKDYDMRVLAR
                     VSFDPDFGLEKK"
     gene            13513..14253
                     /locus_tag="VF226_20500"
     CDS             13513..14253
                     /locus_tag="VF226_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076425.1"
                     /translation="MLRVEGVVGGYGSVQILNGATLKVDEGRIVALLGGNGTGKSTLL
                     KCISGLIKPWAGTISFDGERIDGLRPDLIVRRGLVQVTQGKEAYPAMTVEENLRLGAF
                     VVRNRAKISRALERVYTYFPILKEKRRQLSGTLSGGQLQMLCIGRGLMSEPKMIMLDE
                     PSAALAPQVVLDIFRTISRIARDGMTVLMVEQNVRMALLLASYGYVIKDGVIHIEGPA
                     ESLIHDDNVRLSYLGGTVASQDATLSMN"
     gene            14422..15312
                     /locus_tag="VF226_20505"
     CDS             14422..15312
                     /locus_tag="VF226_20505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076426.1"
                     /translation="MTYFIDLVIVGLSIGMIYALVAIGISLIYSGLDILHFAHGEIYM
                     LGAFIGLMVFHNLGLDYTMTLIVAMILTGLVGVVIERLFYRRLTEAGGGYTVAGMGMI
                     ISGFGMSIVLQNVAFLIWGPKAVPFPVPFGMPVEFGDLSLPPSYIWIVSIGLVLMIGL
                     HVFMKYTRLGLAVRAVAHNKDIAYLMGINVPLMISVIFGIGCALGAAAGVLVGPINYV
                     QILMGLGVIIKAFAAAVVGGFGSLPGAVLGGLLVGVTESLGAGYVSGNYKDIYAFLLL
                     ILVLMIKPSGILGVEAKVKA"
     gene            complement(15431..>15853)
                     /locus_tag="VF226_20510"
     CDS             complement(15431..>15853)
                     /locus_tag="VF226_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018856595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7076427.1"
                     /translation="LGATDVFNAADSDVADSIREATKGGVDVAAELAGSVQALTLAYR
                     ITRRGGTTVTAGLPPPSAMLSIPAVSLVAEERTIKGSYIGTSVPSRDLPRYLGLFRRG
                     RLPIDRLLTHRLPLDDINAGFDRLREGQAVRQIVQFGA"
BASE COUNT         2875 a         4966 c         5046 g         2966 t
ORIGIN      
        1 gaccgggcgg ttggagtgct tgaagccgag ccagaccgcc gagctgatat ctgccattgc
       61 tgagacgtcc gccacctctg ccggcggcgg cgaagcgacc gagcgcctgc ttttgctgga
      121 gcgcaagagc ggcaaacgtc cctttctcgc cgaggtcgcg cccctgcgcg atatcaccgg
      181 cgagctggaa cgcggcttcg ccggcgcggt ggtgttcctg atcgatcctg agaacgcgaa
      241 ggccatttcg acggagcgcc tgtcgcggta ctttgggctc tcgcaagcgg aaagtgaggt
      301 cgcgcgcgcc atggtcgatg ggctttcgac tggcgaaata gccgatctgc gcggtaccaa
      361 ggtcgagacg gtgcgttcgc agttcaaatc catctactcc aagacgggaa tccgccgccg
      421 ggccgatctc gtccgtctcg cggtcagcgt ggatcctccg atcgtacgca gcgccaatgc
      481 aacggaagga gcatcagcgt gagcagcggc tcaggctgca gggtcgagcg ccagggcgcc
      541 gttctgtgga tcaccatcga ccgcccgcag gtgctcaatg cgttggatgc cgcaacgcat
      601 gccgcacttg cggatgcctt cgacacctac gcggctgacc cggagctgcg cgtcgccgta
      661 ctgaccgggg cgggtgagcg cgcattttgc gtgggcagcg atctcaagca gcgtgcgatg
      721 accaatcgcg accatcatcc gccgaccgga tttggcgggc tgacgcaccg cttcgatctc
      781 ttcaaacccg tcatatgcgc ggtgaacgga cttgcgcttg gtggcggggt cgaattgatg
      841 ctggcctgcg acctcgtgat cgctgcggat cacgcggagt tcgccctgcc ggagccgcgt
      901 gtcgggctga ctgccttggg cggaggcggg ctgcagcgat tggcgcgcca gctcccgctg
      961 aagcaggcca tgtggctcgc attgactggc cggcgcatca acgcccatga ggcgagggaa
     1021 atgggtctcg tcaatgaggt ggtgccgcag gccgacctca aggcccgcgc catggcgctc
     1081 agcggcgagc ttctggatat ggcgccgctg gcgctggaag cctccaagca ggtgatgctg
     1141 caaagcctgg cctgcccgga tcttgccact gcgatgaacg cggaatatcc cgcagccaag
     1201 cgcatgttgg cgagtgaaga tgccgtcgag gggcaaggtg cgttcatgga gaagaggaag
     1261 ccggtctgga aagggcggta ggctgccctt cactcgttct tcacccattt caagatgtgc
     1321 gtgccaacgg caaccagcac gccgtcctgg ttcttgatgc tgatgtcagc cgtcgtgcgt
     1381 tggcgctcga gatcgatgct gctgatgcgg taatccacgg tgacggtatc gccaaagtag
     1441 acgggagcca ggaaacggac gcggtcatat ccgagcgaca tcggcgtggc atccgccgta
     1501 tcgcggacgt gagcaatggc aagcgtcgag gctgtcgaca tgaagccgac catcagcgcg
     1561 ccgtgcgctt gaagccgccc gaacttcgat ttctccatgt aggacttgtt gacgtggttc
     1621 ggcgatagat cgccggttat gccagcaaaa aggtatacgt ccgtttcccc gaccgttttg
     1681 gagaatgtcg ctccatcgcc gaccttgacg taaaactcgc tcatctcgaa gatctcccat
     1741 ttgcgtttcc accccgatgc gtcagggcgc gaacgctgtc gtagcgaaag cccgccagga
     1801 acgaaaggca gcaagagaat gcgcgaaacc ctcactggac aggtggccat cgtaaccggt
     1861 gcagcgcgcg gcatcggcct cggcatcgcc gagcggctcg cggccgaggg cgcaaaggtg
     1921 gtgatctggg atcgcgatct tgcgccgctc gagaggtcgc gaaagtcgtt ctcgccggcc
     1981 gctgccgaaa tcgtcgacgt ggcggattac gcatcggtcg agcgcgcttt cgacgcggct
     2041 gttcggtcct tcgggaaagt cgacatcctg gtcaacaatg ccggcgtcaa cgggcctgtc
     2101 gcaccgacct gggactatcc cctcgaagag tgggaccggg tcgtggcgat cgacatgacc
     2161 gccgtttttt attgctgtcg cgtcgcggcc cggcacatgc gcgagcggcg ccaggggcgc
     2221 atcatcacgg tcgcctcgat cgcgggcaag gagggaatgc cctacattcc ggcctattcc
     2281 gctgcgaaag ccggggtcat cggcttctcc aaagcactgg caagggaatt gtgcgacgtc
     2341 ggcgttaccg tcaactgcat cgcgccggcc atcaccgaga cggagctcat gcgcgagatg
     2401 accccggagc atatcgagcg cgcacgcgcc aaaatcccga tggggcgctt cgtgaagatc
     2461 gaggaaatcg ccgccatgtg cgcgttcatc gcgagtcccg actgcagctt cacgactggc
     2521 ttcgtattcg acgtgacggg cggccgggcg acgtactgag cccgccctcg tcagcccccc
     2581 ggaaacagcg aaaatgcttg cttatcgacc ttaaacggcc aatgacttgg gatgcgccgc
     2641 atcacgatgg ggggtcaaat gctcgacgca gggcttttgt cgataaacca ggctacgctg
     2701 atgcaatgct ccttcgagga gagcattgcg gtgctggcac ggcatggagt tcgcgcgacc
     2761 agcatctggc gggacaagct ccgcgaatat ggcttgtcga aggcccagca gctcctcaaa
     2821 agcgaaggtc ttgccgtatc cggattctgc tctggcggcc tgatcacctc gccggatgcc
     2881 cgcgaggcgg ccgaggcgat ggacgaagtg cggcgcgcga tcgacgagac ggcggcggtc
     2941 gaggcgcgct gcctcgtgtt cgtggcgggc ggggtcgatc cgcgcgacaa ggacatcaag
     3001 gcgacacggg cgcgggtcct cgaccggatc gccgagctcg ttccgtacgc gagagcggcg
     3061 aatgtgacga tcgcgctgga gccgctgcac ccgatgacgt gcgcgacgcg ctcggtactg
     3121 accaccgtcg ggctggccaa cgactggtgt gatgtgctta acgcggagga tgtcgtcggc
     3181 atcgcggtcg acacgtacgc cgtgtggtgg gatccgaatc tcgaagctga gatcgcccgc
     3241 gcgggtaagc gcatctgtgc gttccatatc aacgactggc tgcccgatac ccaggatctg
     3301 cggctcgatc gcggtatgat gggcgatggg ctgatcgata ttccccgcat tcgccgcatg
     3361 gtcgaggagg cgggctacaa agggcattgc gaggtcgaga tcttttcggc gcgaaactgg
     3421 tggaagcgcg atcccgatga ggtcgttcgc acgatcctcg agcgctgtca gacggcggtc
     3481 tgacgcacgg catcggcgat cgctggtgtc ctaacccttc gcccggccgt tcgggccgat
     3541 tccagatcaa gcccggcgca ggctctctgc ccatggggag aggtgaaggg agggcctgtt
     3601 gcagccgttt tcgcccaatt cgggaacgct acattcttca ggatctcgga atatcgcgcg
     3661 caagcagctc gcgcagctct ggcgttacct ccggacggat gccgaaccaa agctcgaatc
     3721 ccgtcccggc ctgataaagc agcatgtcga ggccattggc cgtcttcaag ccaagccggc
     3781 gcgcctgcgc gagcaacttc gtttcgagcg gaacgtaatt gatatctgca acgacgccgc
     3841 ccggcgccat cctcgagaga tcgacgtcga ggtctgcgtt acccttgagg ccgagagagg
     3901 tggcgttggc ggcgagagtt gcgccttcga gcaccttgtt ccggtcctcc cagtgctcgg
     3961 ggatgacgcg atccccatag cgctcggcca tatcgacagc tcgactgtac gtgcgattga
     4021 cgacatgaat gcgcgtgacg ccccgctcga ggaggcccca gacgaccgcg cggccagctc
     4081 cgcccgcgcc gatcaccacg gcatggccgc cggactggtc ccagcctggg gcgccggcgt
     4141 cgagcgcgcc gatgaagcct tccacgtccg tattggtcga gcgcagcacg ccgttatcgt
     4201 accagagcgt atttgaggcg ccgacggcgc gggcgcgctc gtcgggttcc gtcagcctga
     4261 acgcggcctc cttgtgcggc aacgtgatgt tggcgccgac gtagccatgg ccgcgcagat
     4321 tgccgatgaa gtcggggaaa tcctcaggcg gcacttcctc gatgcggtag tccccgtcga
     4381 taccgtaatg cttcagccac cagccgtgca ttttgggcga gcgcgagtgc tttgccggcc
     4441 agccgatgac acaggcggct ctggtcgcgt tcatgatgtc aactcctctc gcgcttcagg
     4501 agatcggcca cgcggaccgg ctgcccgctc tcggcggaga tgctgatggc gagcgttgcc
     4561 gccaacgtgc gggtcccatc ggcaccgctc agcacgggtt cggatcgacc acagatgacg
     4621 tccacgaaat tgcgcatttg ctccacgtaa ggatccgccg gcctcacagg aatgcgttcc
     4681 attttcagcg gcagtcccca attctcccgc ccttcctcat gccatctcaa ttcgagtgaa
     4741 ggcacggtga gggagccgcg cgtgccggag atgagatagc agttttcagg ctcgttcgga
     4801 tagaacgggt tctcgcgtga tgtccactcc cagctccagg gcgccgatgc actgtcggag
     4861 atgagcagcg tgccgagggc cccgctggca aaggtgagca cggccgcggc cgtgtcttcg
     4921 acgggaagct cacgagccgt ccgggcggtt tttgcctgga cggtttcgat ttcgccgcag
     4981 agcattcgca ggcaatcgat gtcgtggatg ccgttgatca acacaggtcc gccgcccggc
     5041 tgcccacgcc agctcacgtt gaagtagtcc tttggtttat ggctgagcca cagaccggca
     5101 acggcaagga ccttgccgat tccgccggag cggatcactt cggctgcctt ttgcataatg
     5161 ggattatggc ggcggtggtg gccgacgaga acaggcacgc gcgccctttc agcggcctcg
     5221 acgaggtcca gcgcggctgc aatggagtct gcgatcggtt tctcgaccag caccggaatc
     5281 ttgcgctcga cgcatgcaag gccaacgctc gcatggagct gattgggcgt cgcaataatg
     5341 acgccgtccg gcttctcgcg atccaggagg atgtcgtggc tcgtaaagtg cggcactccg
     5401 agctcgcccg cgagctgcgc ggccgcaggc gccgggtcgg cgatggcagc caatacgtgc
     5461 cgatcgggat cgttcttcag cacctcgatg tgccgcctcc cgatcatacc cgagccgatg
     5521 acggcgatgc gcaccggagc cataggggtc cctcagggat tgaaatgggc tgggttgatg
     5581 agagcggggt cgcgaggcac ctgcggtgcg aactccaggc gttcgagcac gtcgcgacgc
     5641 acgtcgatgc ctggtgccac ctccgtcagg acgagcccat cgtcggagag ccggaatacg
     5701 gctcgctctg tgacatagac gacctcttga ccgcgtcggc gcgagatggc gcctgaaaat
     5761 gtgatctgct cgacctgctt gaccagcttg cagacatcgc cattgcgcgc gatgcgcagc
     5821 ctcccgccgc cgagctccag ctctgttcct ttggtgtcga atgttccgca gaacaccacc
     5881 ttgcgggcat tttgcgcgat gtctatgaag ccgcctggac cgacagtggt gccggcgagc
     5941 ttcgaaacgt tgacgtttcc tgacgcgtcg atctcgccga agccgaggaa tgcaatgtcg
     6001 aggccaccac cggagtagaa gtcgaactgc gaaggcccgt cgatcatggc cgttgcattg
     6061 cgcgcaaagc cgaacagcgt gccgtcgagc aatgcgccac cataggttcc gtgctcgatg
     6121 gtctgatagt agcggtcgag atggccacgc gcggccacca gcttggcgac gccgtccggg
     6181 atgccgaagc catagttcag aacggcaccg tcatatagtt catccgcagc gcgacgggcg
     6241 atcacctggc gcacgctgaa cggcggctcg ggagcgggcg gcagcggacc cttgatctcg
     6301 cctgagagcg tcgggtcgtg gacaacatcg taggtgatgg actgggtcgg atcgaccacg
     6361 accgcatcga cgatggcccc tggtacgcgc accgagcggg ccggcagaac gccgacgtcg
     6421 acggcggtgc gcacctgtgc gatgaccttg ccgccggaat tgtgagccgc gagcgcggcc
     6481 gcgtagatgt cgaggttcgc cgcctcctgg tcgaggctga tgttgccgtg cgcgtcggcg
     6541 aaagacccgc ggatgacggc gatattcaca ggaaaggtcc ggtagcgcag caattcgcgg
     6601 ccatcgatct cgacgacttc gacgaggtcg tccttggcgc gactgttcat ccgcccgccc
     6661 tcgatgcgcg ggtcgacgaa ggtgccgagg cccacgtgcg tgaagagccc cggccgtccg
     6721 gcgccgatct cgcgaaagag ctgcatggtc accccgcccg gcaggacgta ggcttcgatc
     6781 ttgttctctc gagccagggc ctgcatgcgc ggtgaccaga cccagtggcc gccgatgacg
     6841 cgcttcagca tgccctcgtg cgcaaaacaa ttcatgccgc gcgtcttgcg atcgccgatc
     6901 ccgagggcgt gaacgacggt gaggtcgcgt ggatgtccgg tttcgagaaa tcgtttctcg
     6961 acggcagcga acaggcatga ggcttcgaca agtccaccac cgccgccgac gagtgcgacg
     7021 gtgtcgccgt ccttgatcag gccgaccgcc gtcgcagcat cggtgaattt gttgaaagcc
     7081 attcgactgc tcgccctact cgatgacgtc tgcgaagcgc cccgctccgt gtttcagcat
     7141 gctgcgtgcg atcaccatgc gatgaatttc ggaagtgccg tcgaaaatac ggaaaatgcg
     7201 cgcgtcgcgg taaagccgct cgatgggcat gtccttgcaa tagcccatcc cgccgaacac
     7261 ctgcacggcg cggtcggcaa cacggcccag cgtttcggac gcataggcct tcaccatcga
     7321 aatgcgctcg cgggggtcgc ggccttcgtc gatctccgag gcggcgtgga gcaccatgag
     7381 gcgcgcagcg ttgatctcca tggcgctgtc ggcgagcatc tgctggacca tctggaactc
     7441 gccgatcgat ttcccgaact gccggcgctc gcgcgcgtaa tcggtcatca tgcgcagcag
     7501 gcgtgtcgcc ttgccgacgc agcgcgcgcc gatatgggca agacgaatcc tgccaagtgt
     7561 ttccagcgcg agcttgagcc cttctccctc accgccgagc agatgctccg gtccgagctc
     7621 gacgtcttca aaaaacatct cgacgtggct ggtgccgccg agccccatca tcggctggtc
     7681 acggccgatg gtgaagcccg gcattccctt gtccacgagg aacagagaga tgccgcgcgc
     7741 ccctgctgca ggatcggtga cggcggaaac aacgaaaaaa tccgagtaga gcccgtcgct
     7801 gatgaagtgc ttgtggccgt tgaggaccca gccgttgccg cggcgaacgg cgctggtttt
     7861 gatgccggcc gcatccgagc cggcctccgg ctccgtgaat gcgacggaga atgtgcgctc
     7921 accgcggacg gccggtttga gccagcgttc ccgttgtgct tccgtggcga ccagcagcac
     7981 ctcgtagaca ttgccgaaag cacggcgcac cagaatgtcc gtggtgtgcc cgaattgctc
     8041 ctcggcgagg caggtatcga cggccgacaa gccgccgccg ccgtattccg ccgggatgtt
     8101 cacggcataa agcccgagcg cgcgggactt ctcgaaaatt gcacgcgcga ttgcgggatc
     8161 gagctgccgc tttgcctcga cctcctcctc gagcggcacc agctcgtcgt agacgaaacg
     8221 gcgcgccgtt tcgatcagca tgcgctgctc gtcactcaac gcgaagtcca tgggggatct
     8281 gtctcctgct tcagtacacg ccggacgttg cgccgccgtc gaccgcgata ctggtcccct
     8341 ggatgtggcg cgcccgctcg cttgcaagga agacggcgag ctcggcaaca tcctcgggcg
     8401 tgccgaggcg gcgcaggcca ttcttggcca tcgcgcgtgc ccgtacttca tccggcgtgg
     8461 ttttcaaggc tacggcgcgc tgttggaaga gctgttcgac ccgctccgtc tcggtgagcc
     8521 cgggcaggat ggcattgacg ttcacgccgt cgcgcatacc gatgccggac agccccttgc
     8581 tgaagttggc gaaggccgcg ttgacggcgc cgccgaccag gaactcaggg tccgggatgc
     8641 gcgctgcgcc accgatgatg ttgacgacga agccctggga ccgcttcagc atcggccaga
     8701 acagccggct cagccgaacc gcggcgaaga acttcagggc gaagccgtcg tgccagtctt
     8761 cgtcaggctg ctcgagaaac gcgcccgcct tggttgcacc cgccgagttg accagcacat
     8821 cgcaacgccc gagctctgct tggaccttat tgtaaacggc ttcgcacccc tgctgctcgc
     8881 gcaggtcggc agcgacggtg atgggccgtt tcgcgcccct ctcagcgatc gcctcggcag
     8941 ccttgtcgag gttcgccgcg gaggaggccg cgagcaccac ctgtgcaccg cgatctgcga
     9001 atgcggcacc tatggcacga ccgatgccgc ggctgccacc ggtgacaacc acgaccttgc
     9061 cttcaaattc acgtgacatc gattgaccct cagccgttca ggtattgcga ctccagcccc
     9121 ttcacgagag ccaccatggc gtcgatcgtg ggggtcggca cgccgtgcgc gcggccgagg
     9181 cgcgcgacgg cgccgtggat cgtatcaatt tccgtgcgcc gtttgcgtac aatatcctcg
     9241 cgcatcgagg acttggcggc cccggtgccc ccgccggtcg tgtcgacgag cctcatcagg
     9301 atctcccgcg cctcgccggt gtcgagcttg tatccctccg ccgccgcaac cgcggccgcc
     9361 tcgtcgaccg agcggaagac gacctcgcga agctcaggaa cggccatgat ctcgcatgag
     9421 cgcaggtcgg tcacggcgga aatggcgcca agggcgacgt tgccgagaag cttcttccac
     9481 ttttcgcgtt tgatatcggc gctggccgag gtgggcaggc cgtgtcgcgt gaatgtgtta
     9541 gcgatagaga tggcgcgatc agacaatctg ccctgcagct cgccgatata ggtcggcagg
     9601 ttgccaaagt tccgcacaac accgggtgcc tcgacgagac cggcctgtgc cgtcagaccg
     9661 ccgagcacgt tttccgcacc caggatggca cccaggacgt cctcgttgcc gagcccgttc
     9721 tggaagctga tcgccaccgc accgcggccg aacagatgct tgacactctt cgcggccgcc
     9781 tcgttggcga aggatttgca ttggaacagc accacgtcgg cctcggtgag cgacgcagcg
     9841 tccgtcgtag ccttcaccgg gatcgtccgg tccccgccga cgccgacgat gcgcagacct
     9901 cggtcgttga tcgccgcgac gtgctcggcc caggggtcga gcaacgtcac cttgagccca
     9961 tgctccgcaa gcagcccgcc gaacaggccg cccatggcac cggcaccagc gaccacgatc
    10021 tggggctgct cgaatggcat tctgttctct ctccgtgatg tctgctttgc cgcctgtagg
    10081 gctcaggatg cccgcaggaa ttcgcgtact tcggcgtcgc ccatctcttt gccgaggtag
    10141 gcctctatga cgcggtggtc ttccttgatg gctgccggcg gcccttcggc gatcttctcg
    10201 ccgtgatcga gcacggtgat cgtctcgcag gattccatga cgactttcaa aatgtgctcg
    10261 atgatgatga cggtcagccc gtagcggctc ttgagcgcca gcaccgtctc gagaagcttg
    10321 tcgacgttga ccggcgacag gccggcagca ggctcgtcga gcatcagcaa tttcggccgc
    10381 atggcgatgg cgcggcccag ggcgagcaaa cggcgctgtc cgatcgacat ctccgcagcc
    10441 ggcatgtcgg cgtaatcggc aagaccgatg aaggagagca gctccatcat ctcttcccgc
    10501 acgcgcctct cctcggcctg cacccggcgc gtaccgagga tcgcggaggt gatggcgtag
    10561 cggatgtgcg cgtgatgacc gatgagcagg ttctccagca ccgtcatgcc gccgaaaatg
    10621 cgcaggagct ggaatgttcg cgcaataccg agccgcgcaa tcacgtgcgg ctcttcgttg
    10681 gtgatgtccc gcccgtcgaa gacgactttg ctgccgggat cggaccggta aacacccgaa
    10741 atgagattga acagcgtgct tttgccggag ccgttcgggc caatgatgcc gcgcacctcg
    10801 ccttcgcgga tcgcgagatc gaggttgttg accgcgacaa ggccaccgaa gctcttggtc
    10861 aggcttttcg tctcaagcag catgcgtctt cacctgccgg atggcgttga agcgccgccg
    10921 cacgagatac tggtcgatca gcccgccgat ccccttgggc atgtagagca cggtcagcac
    10981 gatcacgccg ccgaacatca gcatctggta ctgatagtag tcgcgcgtga agtcgtggat
    11041 gatggtgacg aggatcgcgc cgacgacccc gccccaaatg tggccgatac cgccgaaaac
    11101 gatcatcaac agcacggtca ccatctccat gatcgtgtaa ttgttcggat ggatgaagcc
    11161 cggcgtcatg tgtgccagca ggctgcccgc gcatcccgcg taaagcgcgc tgatgacgaa
    11221 tgcgatgacc ttgtacttgc ggacatcgat gccggtggcc gccgccgcaa gcggatcttc
    11281 gcgcagggcc ttgaaggcac gcccgatgtc cgaacgcaaa atgctgaatg agaaatacat
    11341 gccgataagg gcgaggggca tcacgatgta gaagtaggac gcatgcgtat tgagcgcatg
    11401 tccgaagaaa tagggcgggg gaatgttctc gtacccgatg gctgcaccga gataatcggt
    11461 ggcagaaatg aaaatgcgca ccgactctgc gagcccgagc gttgcaagcg ccagataggc
    11521 accctcgagc cgaaacgagg gaatgcccac gatggccccc gccagtcccg caatgaggat
    11581 ggccgatggg aacgcgagcc agaacggcag gccgaatttc gtcgagagca cggccgagct
    11641 gtacgctccg atggcggcaa tggcgatgtg gccgaccgtg acctggccgg caaacccctt
    11701 cacgatgttc agcccaacgg caaggatgat gtttatgcag atgacgttga caatgtactg
    11761 ctggtaggag ttgggcatca gcaaaggtaa aggcaggagt acgacgaacc cgatgatcca
    11821 ggggacgatc gggtgcatcg cgcctagtcg gctgaatacc gacggacgca ccacggacgg
    11881 ctccgcaata ttttcgacgc tcaaatcgga agcctcccct tctcatccgt catgccggaa
    11941 ccctgccgaa cgcaccatac gtgcacctca ccctctgtca tcccggggtt ttgccccgaa
    12001 acaacccatt caattcaaac ggcggaaccc cggctttcgc tttcgttctc cccctagtcc
    12061 gcggccaggg atcccggcga cgcgcgccgg gatgccgttg caggtttatg gccgaagatg
    12121 gcgtacagtg gcccttccgg gatgatgagc ccgtggctca tcatgccgac ctctacttct
    12181 tctcgagccc gaaatccgga tcgaacgaca cccgggcaag gacgcgcatg tcgtaatcct
    12241 ttccgccctt cgtgtattcg accaggatgc ccgtgcgatt gccgtcgcgg cattgcggcg
    12301 tcggatcggc gcagaaattg atcgggcccg aggccaggcc atggtaatcc ttgatcgacg
    12361 ccagagcatc cctgatcgca cggcgatcct cggcgagcga ctcctccgtg agcttcaggt
    12421 tcgccctgct cagtgccagc ttgacgagct gcgcggtctc gtaagcctgg ctgaagtcgt
    12481 ggtccgggca gcggaccttg tacttctcct ggaccatctt cacgaactcc tgcgcgatgg
    12541 gccgctcatc cttgcaggag aaagctgcag caaaggtaag gcccttcgcg gcctcgcccg
    12601 cgagctcggg cacaggcgca ttcgacgctg cggaggcagc aacgcggcgc acccgcgagg
    12661 gtatcccgac ctcgtaggac tgctgcgtga tgcgggcgat ggcctcgagc tggccgccga
    12721 tcgccaacac atccggattg agggacttga tcttgagcag gtgagagcgg aaatcgagat
    12781 ccgtctcctg catctgcgcc tcatacaccg gctcgacctg gtaggtccgg cgcatgaagt
    12841 tcatgtaatc cttggcaaaa ccgatgccgg cgccgtccgt cgtgtagagg taggcgacct
    12901 tcttgccgac gttctcagaa agatactttg cgtgcacgga cgcgtaaaag cgctcggcaa
    12961 cggccgtgcg aaacacccag gcgctgccct tctgggtgat gctggagttg gtggagtgcg
    13021 ggacgatctg cggcacttcc tccttggccg tgatgtcgac catcggcaag gacacggagc
    13081 tgcaggtcga gccgatgacg aggtgaacct tgtgctggtg tatgaagcga ttgacgttgg
    13141 ctatgccctt gtccggcttg cactcgtcgt cgagcaggat cacctcgacc ttcttgccgt
    13201 tgatcccgcc actggcgttc acggcctcca gctcatcctt cgtcatctgg ccgtacttga
    13261 ggccgttctc gacaaccatc cggagcgtaa tgccgatccg gatattgtcc tcggccagcg
    13321 ccggaaccgc cgccacgccc aacgcgagcg ccgcaagggg caccgcatgc ctcatcgcct
    13381 tgaacatcgc ttcctccctg gccggttttg gaaccatgcc aattctttgc cgattgcgaa
    13441 tgggctttac ctagccgcac cattcccctc taattagcag actttacgcc cggataggca
    13501 agaggaaaca agatgctgcg tgtcgaaggc gtcgttggcg gatatggcag tgtacagatc
    13561 ctcaatggcg cgacgttgaa ggtcgacgag ggtcggatcg ttgccttgct cggcggtaac
    13621 ggcaccggca aatcgaccct gctgaagtgc atctccgggc tgatcaaacc ctgggccggg
    13681 actatcagtt tcgacggaga gaggatcgac ggcctcaggc cggacctcat cgttcggcgc
    13741 ggtctcgtac aagtgacgca gggaaaggag gcctatccgg ccatgacggt cgaggagaac
    13801 ctgcgtctcg gtgccttcgt ggttcggaac agggccaaga tttcaagagc cctcgaacgc
    13861 gtctacacgt attttccgat cctgaaggaa aagcgccggc agctttccgg cacgctttcc
    13921 ggggggcagc tccaaatgct ctgcatcggg cgcgggctca tgtccgagcc gaagatgatc
    13981 atgctggacg agccgagcgc cgctcttgcc ccgcaggtcg ttctcgacat cttccgcacc
    14041 atctcgcgca tcgcgcggga cggcatgacg gtgctgatgg tggagcagaa cgtgcgcatg
    14101 gcgctgctgc tcgcgagtta tggctacgtg atcaaggatg gcgtcattca catcgagggg
    14161 ccggccgagt cgctcatcca tgacgacaat gtacggctgt cctacctcgg cggcacggtg
    14221 gcgagccagg atgctacgct cagcatgaac tagcgtgtcc gactgccagc gcgcgcaggg
    14281 acggacttcg gagccaaatg aacactagag gatcgagttg cttgcgtgat tgagatcgag
    14341 aaattcgctc acggccggac cagcacaggc ggaatttctt gattatcaca ctgagaacaa
    14401 aaagtccttt tgggaggacg cgtgacctac ttcatcgatc tcgtgatcgt cgggctgtcc
    14461 atcggcatga tctatgccct cgtggcgatc ggcatttccc tgatctattc cgggctcgat
    14521 attctccatt tcgcccacgg cgagatttat atgctgggcg cgttcatcgg gctcatggtg
    14581 ttccacaatc tgggcctcga ctacacgatg acgcttatcg tggcgatgat cctgaccggc
    14641 ctcgtcgggg tcgtcatcga gcggctgttc tatcgccgac tgacggaggc tggcggtggc
    14701 tacaccgtcg ctggcatggg catgatcatc tccgggttcg gcatgtccat cgtgctgcag
    14761 aatgtggcgt ttctcatctg gggaccgaag gcggtcccat tcccggtgcc gttcggcatg
    14821 ccagtcgagt tcggcgacct gtcgctgccg ccctcctaca tctggatcgt atcgatcgga
    14881 ctcgtgctga tgatcggtct gcacgtgttc atgaaataca ctcgcctcgg cctggcggta
    14941 cgcgccgtcg cccacaacaa ggacatcgcc tacctgatgg gcatcaacgt gccgctgatg
    15001 atctcggtga tcttcggcat tggttgcgcg cttggcgctg cggcgggggt gctcgtcggc
    15061 ccgatcaatt acgtccagat cctcatgggc ctcggcgtca tcatcaaagc gttcgcagcc
    15121 gccgtcgtgg gcggcttcgg cagcttgcct ggcgccgtgc ttggcggtct gctggtaggc
    15181 gtcaccgaga gccttggagc cggatatgtc tcgggcaact acaaggacat ctacgcgttc
    15241 ctgctgctca tcctggttct gatgatcaag ccgtcgggga ttctaggcgt cgaagcgaag
    15301 gtcaaggcct gaatcaagaa caccgatcat gtgttcccgc ttgcgtcgct cccaaacgtc
    15361 tcccgcccgg atgggtttgt caggcccgcc ccggagagcc cagcacgctc attaaggtga
    15421 gggcagatgc tcacgctccg aactggacga tctgtcgtac ggcctgacct tcgcggaggc
    15481 ggtcgaagcc tgcgttgatg tcgtcgagcg gcagtcgatg cgtcagcagg cgatcgatgg
    15541 gcagacgacc gcgcctgaac agcccgagat agcggggcag atcgcgcgac ggcacggagg
    15601 tgccgatgta gctccctttg atcgtgcgct cctctgcaac gaggctgacc gccgggatcg
    15661 acagcatggc gctcggcggc ggcaatcccg ccgtcaccgt cgtgccgccg cggcgcgtga
    15721 tgcggtaggc gagcgtcagc gcctgaacgg agcctgccag ctccgcagcc acgtcaacac
    15781 cgcccttcgt tgcctcgcgg atgctgtctg ccacgtcaga atccgcggca ttgaaaacgt
    15841 cagtggcacc gag
//
LOCUS       DASVDU010000306         6278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_475358, whole genome shotgun sequence.
ACCESSION   DASVDU010000306 DASVDU010000000
VERSION     DASVDU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475358"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1017)
                     /locus_tag="VF226_20515"
     CDS             complement(4..1017)
                     /locus_tag="VF226_20515"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7076428.1"
                     /translation="MTTKAAPDKPASDRVETLTVKRDEAGMRLDRWFRTHFPEVTYTY
                     LQKLLRTGQVRVDSRRAQANDRLEAGAQVRVPKAARTPAKRPPSVAPPLGLSKADRDF
                     IERMILYEDDDVLVLNKPFGIAVQGGTGTKRHIDGLLAGMVDRFGDRPRLVHRLDRDT
                     TGVLLVAKRRDIAAKLGRVFQTRSAAKTYWALVKGVPKPPQGKVEAALVKAAGPEGDR
                     VRKALPGEQDKAMHATTHYSVIDRVGTKAAWVSLKPVTGRQHQLRAHMALIGHPIVGD
                     NKYEGGLDLPLEDIEPKLHLHARRLVLPHPRGGKIDVTAPLPDHMRRTWELLGLDEGK
                     WDR"
     gene            complement(1014..1394)
                     /gene="crcB"
                     /locus_tag="VF226_20520"
     CDS             complement(1014..1394)
                     /gene="crcB"
                     /locus_tag="VF226_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX7076429.1"
                     /translation="MKLLLLACAGGALGAGARYLVYVEIARRWSSGFPWPTLSVNVAG
                     SLLIGVLAALFLERWPETVGLRVFLITGVLGGFTTFSAFSLDFMSLMERGQAGVAGMY
                     AAASVVLSIAACWAGLSIARSLMP"
     gene            complement(1473..1706)
                     /locus_tag="VF226_20525"
     CDS             complement(1473..1706)
                     /locus_tag="VF226_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076430.1"
                     /translation="MRYLIAMIFAIVAAAAATVFVSSPIATWVVDRMAFESPDEVADL
                     HSMVFMGVNLVALAIGWTIGWWLGNFEGSQSET"
     gene            complement(1792..2340)
                     /locus_tag="VF226_20530"
     CDS             complement(1792..2340)
                     /locus_tag="VF226_20530"
                     /inference="COORDINATES: protein motif:HMM:NF025478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector amidase Tae4 family protein"
                     /protein_id="HEX7076431.1"
                     /translation="MVAFRDLWRGHPINESVQSPCIAPHDLTNLEGTPVAGGFPVFSN
                     QCAIRMGVALKRAGVTASQLPGAAHCSVHSREEMHFINATQLANAIARANLPGVGPRE
                     IISGPDAAKFYPKIFGRTGIIFIQDYWRRSSDPIGRPTGDHIDVWNGYRSSAKWLMEW
                     FSWLGYYSNYAEAREIWFWEVK"
     gene            complement(2449..3762)
                     /locus_tag="VF226_20535"
     CDS             complement(2449..3762)
                     /locus_tag="VF226_20535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX7076432.1"
                     /translation="MDNLFQAAGLEKRAPRPLADRLRPKSLSEVVGQDHLVGPEGTLT
                     RMVRSGRLQSMILWGPPGTGKTTIARLLAHETALEFEQLSAIFSGVQDLRKAFERAKS
                     RRAVGKGTLLFIDEIHRFNRSQQDSFLPHVEDGTITLVGATTENPSFELNAALLSRAA
                     VLVFHRLDEAALGELLRRAEAEEGRPLPVDEEARAALIAMADGDGRSVLNLAEEVFAA
                     VPVGGAPLSREALTSLVQRRAPLYDKSRDGHYNLISALHKAVRGSDPDAALYWLARML
                     DGGEDPLYLARRLVRMAIEDVGLADPQAVVQALAAKDVYDFLGSPEGELALAQATIYL
                     AVAPKSNGAYVAFKRAMRAAKEGGSLSPPKHILNAPTRLMAEEGYGAGYEYDHDTPEG
                     FSGQDYFPEGMGRQQFYDPPERGFEREIRKRLAYFAELRRRRQKE"
     gene            complement(3771..5159)
                     /locus_tag="VF226_20540"
     CDS             complement(3771..5159)
                     /locus_tag="VF226_20540"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEX7076433.1"
                     /translation="MTISLLAAASLAFDAAEAEQRIPPPSRQAIQYSYAPIVKRAAPA
                     VVNVYVRSRVQTGLPPLFDDPFFRRFFGDSFGMPRERIQNSLGSGVIVSPDGIVVTNN
                     HVIQGGQLTEIRVALADRREFDAKVILQDEKTDIAVLRLEANGETFPTLELADSDALE
                     VGDLVLAIGNPFGVGQTVTSGIISALARTEVARSDAQVFIQTDAAINPGNSGGALVDM
                     HGRLVGINTAIYSRSGGSLGIGFAIPTNLVRLYVESALTGRKVERPWLGARLEAVTRD
                     IAESLGLGRVAGALVMRVYANGPAAEAGIEPGDVIVAVDGFDVADPRAVYYRLVTRGI
                     GKTARVEVIRKRRRISFNLPLRPAPAPTQDDVRNLSGPHPFDGARVANMLPSMAEELG
                     LEDQEGVVVLSVRSGSTAANIFRPGDIILRIGDQPIDDVRDLDRILQLHPRVWQITIK
                     REGRILQFQFMG"
     gene            complement(5572..5991)
                     /gene="rplQ"
                     /locus_tag="VF226_20545"
     CDS             complement(5572..5991)
                     /gene="rplQ"
                     /locus_tag="VF226_20545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7076434.1"
                     /translation="MRHRKLGRKFSRDSGHRQAMLSNMAAALIRHEQIVTTLPKAKDL
                     RRVVDKYITLAKRGDLNSRRLAASRLRDEEMTKKLFDVLGPRYKDRAGGYTRVLKAGF
                     RFGDSAPRAVIELVDRDTDAKGKDDRARVAASEQQES"
     gene            complement(6122..>6278)
                     /locus_tag="VF226_20550"
     CDS             complement(6122..>6278)
                     /locus_tag="VF226_20550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HEX7076435.1"
                     /translation="AEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPNWPPDNIEELA
                     KRFEDQY"
BASE COUNT         1116 a         2056 c         2010 g         1096 t
ORIGIN      
        1 ccctcacctg tcccacttcc cctcgtccag ccccaggagc tcccaggtcc gccgcatatg
       61 gtccggcagc ggagcggtca catcgatttt gccgccgcgc ggatggggga ggacgaggcg
      121 gcgggcgtgg aggtggagct tgggctcgat gtcctccagc ggcaggtcga gaccgccttc
      181 gtacttgttg tcgccgacga tgggatggcc tatgagcgcc atgtgcgcgc ggagctgatg
      241 ctggcgtccg gtgacgggtt tcagcgagac ccacgcggcc ttggtgccga cgcgatcgat
      301 caccgaataa tgtgtcgtcg cgtgcatggc cttgtcctgt tccccgggca acgccttgcg
      361 cacgcgatcg ccttccggac cggccgcctt cacgagggcg gcctcgacct tgccctgcgg
      421 cggcttcggc acccctttca ccagcgccca gtaggtcttg gcggcggagc gggtctggaa
      481 cacgcgcccc agcttggcgg caatgtcgcg gcgcttggcg acgagcagca cgccggtcgt
      541 atcacggtcg aggcggtgga cgagccgcgg gcgatcgccg aagcgatcga ccatcccagc
      601 cagcaggccg tcgatgtgcc gcttcgtgcc cgtgccgccc tgcaccgcaa tgccgaacgg
      661 cttgttgagc acgaggacgt cgtcgtcctc gtaaaggatc atgcgctcga tgaaatcgcg
      721 atcggccttg gaaagcccaa gcggcggcgc caccgagggc ggccgtttcg ccggcgtgcg
      781 ggccgctttc ggcacgcgca cctgggcgcc tgcttcgagc cggtcattgg cctgcgcgcg
      841 gcggctgtcg accctcacct gaccggtgcg taacagcttt tgaaggtagg tataggtgac
      901 ctcggggaag tgggtgcgga accagcggtc gagccgcatg cccgcctcgt cacgcttcac
      961 cgtgagcgtc tcgacgcggt cgctcgcggg cttgtctggc gcggctttcg tcgtcatggc
     1021 atcagcgacc gcgcgatgga gaggcccgcc caacaggcgg cgatcgagag gacgacggag
     1081 gcggccgcgt acatgcctgc cacgccggcc tgcccacgct ccatcagcga catgaaatcc
     1141 agcgagaatg ccgagaacgt ggtgaacccg ccgagaacac cggtgatgag aaaaacccgc
     1201 aatccgaccg tttccggcca acgctcgagg aacagagcgg cgagcacgcc gatcagcaag
     1261 gagcccgcca cgttcacgct gagcgtgggc cagggaaaac cgctgctcca cctgcgcgcg
     1321 atttcgacat agacgagata acgtgcaccc gcgccgaggg cacccccggc acaggcgagc
     1381 agtaacagct tcatgaggtc cggtcccgat tgcccgggcg agatataagc ggcccggcct
     1441 gcgagagcaa aggcgtgttc tgctgtggcc gatcaggtct cgctctgcga cccctcgaag
     1501 ttgccaagcc accagccgat cgtccaaccg atggcgaggg caacaaggtt cacgcccatg
     1561 aacaccatcg aatgcaggtc ggccacttcg tccgggcttt cgaacgccat ccggtcgacg
     1621 acccacgtgg caatcggcga gctcacgaag actgtggctg ctgccgcagc gacgatcgcg
     1681 aagatcatcg cgatgagata tcgcattcgc tggtctccta gagcgcctct tctcgcttcg
     1741 ctggccctct ccctgggaga ggtcaacgga tcagctcatc tcctgccgct ctcacttcac
     1801 ctcccagaac cagatctccc gcgcctcggc gtagttcgag tagtagccga gccacgagaa
     1861 ccactccatc agccacttcg cggatgagcg atagccgttc cagacatcga tgtgatctcc
     1921 ggtgggacgg ccgatgggat cggagctgcg gcgccagtag tcctggatga aaatgatgcc
     1981 ggtgcggccg aatatcttgg gatagaactt cgccgcatcc gggccggaga tgatctcgcg
     2041 agggcccacg ccggggaggt tcgcgcgagc gatggcgttg gcgagctgag tggcgttgat
     2101 gaagtgcatc tcctcgcgcg agtggaccga gcagtgtgcg gccccgggca gctggctcgc
     2161 cgtcacgcca gcccgcttca atgccacgcc catgcggatc gcgcactgat tggaaaagac
     2221 gggaaagccc ccggcaaccg gcgtgccttc gaggttcgtg aggtcatgag gcgcaatgca
     2281 cggcgactgg accgattcgt tgatcggatg accccgccat aggtctcgga acgcgaccac
     2341 ggtgcccctc cctttcaagg tcaggcgcac actacaccag ataggctggc gacgaacagt
     2401 cccgtccgcc cggctgggaa atgagatcag ccctgggtgt ggcgcggcct actccttttg
     2461 ccgccgacgg cgcagctcgg caaagtaggc gaggcgcttg cggatctcgc gctcgaagcc
     2521 gcgctcgggc ggatcgtaga actgctgccg gcccatgcct tccggaaagt agtcctgccc
     2581 cgagaagcct tccggcgtgt cgtggtcgta ctcgtagccg gcgccgtatc cttcctcggc
     2641 catcaatcgg gtcggcgcat tcaggatgtg cttcggcggg ctcaagctgc cgccttcctt
     2701 cgccgcgcgc atggctcgct tgaacgcaac ataggcgccg ttcgatttcg gcgcgaccgc
     2761 caggtagatg gtcgcctgcg ccaacgccag ctcgccctcg gggctgccga ggaaatcgta
     2821 gacgtccttc gccgcgagcg cctgtaccac cgcctgcgga tcagcgaggc cgacatcctc
     2881 gatggccatc cgcaccagac ggcgggcgag gtacaacggg tcctcgccac catcgagcat
     2941 tcgcgcgagc cagtagagcg ctgcatctgg atccgagccg cgcaccgctt tatgcagagc
     3001 actgatgaga ttgtaatggc cgtcgcggga cttgtcgtag aggggggcgc ggcgctgcac
     3061 gagactggtc agcgcctctc tcgacagagg cgcgccaccg accggaacgg ccgcgaagac
     3121 ctcctcggca aggttcagca cggaccggcc gtcgccatca gccatggcga tgagtgcagc
     3181 gcgcgcctcc tcgtcgaccg gcaacggccg gccttcctcg gcctctgcgc ggcgcagcag
     3241 ctctccgagc gctgcctcat cgagccgatg gaagacgaga acggccgcgc gtgacagcag
     3301 ggccgcgttg agctcgaatg acgggttctc cgtcgtcgcg ccgaccaatg tgatcgtgcc
     3361 gtcctccacg tgcggcagga agctgtcctg ctgagaccgg ttgaaccggt ggatctcgtc
     3421 gatgaacagc aacgtgccct tcccgacggc acggcgcgac ttcgccctct cgaaggcctt
     3481 tctgagatcc tgaacgcccg aaaagattgc cgagagctgc tcgaactcga gcgccgtctc
     3541 atgcgccaac aggcgcgcta tggtcgtttt tcctgtgcca ggcggccccc acaggatcat
     3601 gctctgcaga cggcctgagc ggaccatgcg cgtcagcgtg ccctcggggc cgacaaggtg
     3661 atcctggccg acgacctcgc tcagcgattt ggggcgcagc ctgtcggcca gcgggcgcgg
     3721 cgcacgcttt tccagtcctg ccgcctggaa gagattgtcc aaaggcttgt tcaccccatg
     3781 aactgaaact gcagtatacg tccttcccgc ttgatcgtga tctgccagac gcgagggtgc
     3841 aactgcaaga tgcggtcgag atcccgaacg tcgtcgatcg gctgatcgcc gatgcgcagg
     3901 atgatgtcgc ccggccggaa gatgttggcc gccgtcgagc cgctgcgcac cgagagcacg
     3961 acaaccccct cctggtcctc gagccccagc tcctccgcca tcgaaggcag catattggcc
     4021 acccgcgcgc catcaaacgg atgcgggccg gaaaggttgc gcacatcgtc ctgcgttggc
     4081 gccggcgctg gcctgagcgg cagattgaag gagatgcgcc gcctcttccg tatgacttcg
     4141 acgcgtgccg tcttgccgat gccgcgcgtc acgagccggt agtagacggc acgcgggtcg
     4201 gcaacgtcga acccgtccac ggccacgatc acgtcccccg gctcgatgcc cgcttcagcc
     4261 gccggtccgt tcgcgtaaac ccgcatgacc agcgcgcccg cgacacggcc gaggcccaag
     4321 ctttcggcga tatcgcgcgt gacggcctcg agccgcgcgc cgagccatgg ccgctcgacc
     4381 ttgcgacccg ttaatgcgct ttcgacgtaa aggcgcacca gatttgtcgg gatggcaaac
     4441 ccgatgccga gcgagccacc cgatcgcgaa tagatggccg tgttgatgcc gaccagccgg
     4501 ccatgcatgt cgaccagcgc gccacccgag ttaccggggt tgatggcggc atcggtctgg
     4561 atgaagacct gtgcgtcaga acgggcgacc tccgttcgcg ccagcgccga gatgatgccg
     4621 ctcgttaccg tctgcccgac cccgaagggg ttgccgatgg ccagcaccag gtcaccgacc
     4681 tccaaggcat cggagtcggc gagctcgagg gtggggaatg tctcaccatt ggcctccagc
     4741 cggagcacgg caatatcggt cttctcatcc tgaaggatga ccttggcgtc gaactcgcgc
     4801 cggtcagcga gagcgacgcg aatctccgtc agctgaccgc cctggatgac gtgattgttg
     4861 gtgacgacga tgccgtcagg gctcacgatg acaccggagc ccaacgaatt ctggatccgc
     4921 tcgcgcggca ttccgaagct gtcaccgaag aagcggcgaa agaacgggtc atcgaacagg
     4981 ggtgggagcc ccgtctgcac gcgactacgc acgtagacgt tcacgaccgc cggtgcagcg
     5041 cgcttcacga tcggcgcata ggagtactga atcgcttggc gggatggcgg aggaatgcgc
     5101 tgctcagctt cggcggcatc gaatgcaagt gacgctgctg ccagaagcga aatcgtcata
     5161 gcagcgatcc gcggccaggc agcgcagtag cgacatgtca tgaagcccac tccgcgtggt
     5221 gatttccgca atgcagccag ggcgtggaga gatcctcgca tgggtgctag atataaccag
     5281 caggggcgaa ccattgaagc aaacacgccg caatcccggc aactgtgagt gaaagatcgc
     5341 gttcagcgca acgacggcat tagaggcttg ttgcatcacg ctcggaatga gtcgaatgag
     5401 ccgaggctgc gctgatgctt cgcatggcaa gccgcatcgg cgcgccgtcc gcgcgtcgat
     5461 caatatgcct agccgacaaa ttcgaaaaat ggcaacagcc ggcggggtct gatgccgagg
     5521 acacctccag ttgcttcaac ctggaggttg ggcggggcga tcggcgcccc gctacgactc
     5581 ctgctgctcg ctggcagcga cgcgggctct gtcgtccttg cccttggcat ccgtatcgcg
     5641 atcgacgagc tcgatgacgg ctcgcggtgc gctgtcgccg aaacggaatc ccgccttcag
     5701 cacgcgggta tatccgccag ctcgatcctt gtaacgggga ccgagcacat cgaacagctt
     5761 cttggtcatc tcttcatcgc ggagccgcga cgccgcaagc cggcgcgagt tgagatcgcc
     5821 gcgctttgcg agcgtgatgt acttgtccac cacgcgccgc aagtccttgg ccttcggcag
     5881 ggtcgtcacg atctgctcgt ggcggatgag ggctgcagcc atgttggaga gcatcgcctg
     5941 gcgatgcccg ctgtcgcggc tgaacttccg gccgagtttc cggtgtctca ttgtcgtcgt
     6001 ccttcgctgt ggttgttacg gggaggttct ttggcctcgc ctcttgggag cgaatagtga
     6061 gtagcgaata gcgaatagaa cgaaatccta ttcgccattc gccatttcgc cattcgctca
     6121 atcaatactg atcctcgaac cgcttcgcca gctcctcgat gttgtccggc ggccagttgg
     6181 gcacttccat gccgaggtgc aggcccatgg aggccagcac ttccttgatc tcgttcagcg
     6241 acttgcggcc gaagttcggc gtgcgcagca tctccgcc
//
LOCUS       DASVDU010000307        34166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1443508, whole genome shotgun sequence.
ACCESSION   DASVDU010000307 DASVDU010000000
VERSION     DASVDU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 34166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34166
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443508"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1605)
                     /locus_tag="VF226_20555"
     CDS             complement(82..1605)
                     /locus_tag="VF226_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7076436.1"
                     /translation="MLMSAAEYRESLRRYRPRVFVNGERVESVADEPRLAPGINAIGI
                     TYDFALKEQHKPLMLARQGTSGKVVNRMLHINETSQDLLYKLEAVRLVCRESGCAMRY
                     LSHDSLNAVFQATRRTDLNHGTDYHQRFLAYLHRIQDEDLTVAVAMTDAKGDRSKRPG
                     AQSNPDVYVHIKERRPDGIVIRGTKAIVTGAPYVHEFLVMPCRTMTAEDRDFAVCCAV
                     PVDAEGVTIVARPAGRPGEAAAKFSAKYGQSTGVVMFDDVFVPWDRVFLAGEHEEGGF
                     LTTSYATHHRHSCIGARAGFGDLLIGAGVLMLESNGLDPDRHGHIRDAMVELITITES
                     FFACGVAASVYGMKDPAGSIMPDAVFSNIGKLLLATKIYDMHRLAHYVSGGLVVALPG
                     PDEDHNPETKASLSAVLAGRPDIPAEKRMEVARLIEDLTASHQGGWYSLISLHGGGSP
                     EAMRREIWRNYPLMDKRELVERLLDRGVLDVGQRVSKQPGRCCVTGCEVPEVPKAAE"
     gene            1694..2227
                     /locus_tag="VF226_20560"
     CDS             1694..2227
                     /locus_tag="VF226_20560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7076437.1"
                     /translation="MRFAPETSVLLLIDFQARLMPVICDGDIAVANARRLAEAARLLG
                     VPIRATEQNPAGLGRNVGQLAPFAERTLTKRYFDATREAEWAGFIPEGRPHIVVAGCE
                     THVCVMQTVLGMLEKGLQVRLVRDAVGSRTPENREAALHRAERHGAELVTTEMVVFEW
                     LATSDHPRFREALALVK"
     gene            2315..2974
                     /locus_tag="VF226_20565"
     CDS             2315..2974
                     /locus_tag="VF226_20565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7076438.1"
                     /translation="MVANEGVLIIGEDTFVTGKIRNCRRIEIFGRVHGDVTADTVHIH
                     EGGRLDGKARAENADVHGVLQGNVTVRNLINIRSSGSVSGNVQYGQLAMEVGGSLSAE
                     VRNVPPSIGGDLDLSVYRGRSVGVTLEDLTAIDPDDEAQDLIYGVSNAKNGFVALTHA
                     PAEAVTSFTQADLEAGKVIFRHDGSATDWASFDVVVTDASGANSGSPQTVKVTVHSRS
                     G"
     gene            complement(3019..3855)
                     /gene="panB"
                     /locus_tag="VF226_20570"
     CDS             complement(3019..3855)
                     /gene="panB"
                     /locus_tag="VF226_20570"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX7076439.1"
                     /translation="MSSQTVVKRTTSVDIRARKGGTPIVSLTAYSANIAAIADSHVDF
                     LLVGDSVGMVVHGFDTTVPVPLELMIMHGSAVVRGTRRALVVVDLPFGSYEESPEMAF
                     RNAARILKETGCGAVKLEGGRQMAETIRFLVERGIPVMGHVGLTPQAINTLGGFRAQG
                     RTEEQWREIEADAIAVSDAGAFAVVVEAVAEPLAAKLTNTIPVPTIGIGASAACDGQI
                     LVMNDMLGMSARVPKFVRQFGQIGAAIDRAIADYAASVRDRSFPAPEHTYGMKSASKP
                     QP"
     gene            3997..5262
                     /gene="otnK"
                     /locus_tag="VF226_20575"
     CDS             3997..5262
                     /gene="otnK"
                     /locus_tag="VF226_20575"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX7076440.1"
                     /translation="MLLGCIADDLTGATDLALMLTRNGMRTVQVMGVPGPEAPLDNAD
                     AVVVALKSRTNPVDEAVRWSLASADALLAHGARQLYFKYCSTFDSTEKGNIGPVAEAL
                     LEKVKGEIALVCPAFPANKRTVYMGHLFVGTVPLHESPMKDHPLTPMRDSNLVRVLQR
                     QTRLNVGLVPYDVVDQGADATRAAFNEAAKEGTRFAVVDAISDRHLIAMGTAAADHVL
                     ITGGSGVAMGLPDNFARKGLLEKTEPMGHISAPAGRSLIIAGSCSEATRGQVRAAEAA
                     GLPAFKVDPLAIARGKLAAPDMVSWALKQPGDRPVLIYSSDDPANVRAVQTELGREAA
                     GALVERELAAAAKELVDKGFTRLIVAGGETSGAVVGALGVKAIEIGPEIDPGVPWTRS
                     IGGRGLVLALKSGNFGAPDFFLKAWNLIE"
     gene            5259..5897
                     /gene="otnC"
                     /locus_tag="VF226_20580"
     CDS             5259..5897
                     /gene="otnC"
                     /locus_tag="VF226_20580"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HEX7076441.1"
                     /translation="MSAESKLRERICAAGASLFDRGLTHGSTGNISIRLEDGGWLMTP
                     TGSSLGTLDPARLSKLDPAGQLVSGDPPTKEAFLHTTMYSERPKAGAVVHLHSTYSVA
                     VSCLADIDPSNCLPPITAYYVMRIGTLPLVPYFPPGDHSLAEAVGKLAGRHHAVLLAN
                     HGPVVAGSSLSAAMDAIEELEETARLFLLLRDRPVRTLSEAQVAELRRRFPN"
     gene            5944..6693
                     /locus_tag="VF226_20585"
     CDS             5944..6693
                     /locus_tag="VF226_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076442.1"
                     /translation="MANKIDLENRVAVITGGAQGFGRASAERFIASGAKVAIWDRDFA
                     LAEKTAAELGSNVKAFNCDVTDAKSVDAARDATLSAFGKIDILVNNAGIAGPTAKVWE
                     YSLEELQMTMRVNLEGPWLCCKAIVPGMIAQNYGRIVNIASIAGKEGNPNASAYSASK
                     AALIALTKSLAKEVATYDISVNAIAPAAARTAILNQVTEEHINYMLSKIPRGRLVTVE
                     EVAALVAWAASSDCSFTTGQVLDISGGRATY"
     gene            6718..7446
                     /locus_tag="VF226_20590"
     CDS             6718..7446
                     /locus_tag="VF226_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7076443.1"
                     /translation="MATQTIFILLGAFAGGFVNGMTGFGTGLTALAFWLHVIGPALAA
                     PLVLTCSVIAQLQTLPHIWRELDWRRLMPFILGGLAGVPIGTQLLTEIDPKVFKFVMG
                     IVLICYSSSMLVLKPRFGAKWGGRVADGLVGLSGGILGGFAGLSGVLPTIWAAIRGWS
                     KPERRGVFQAFNLTILGTALIAYAFAGLLTAEVGSLILTAVPGTLIGSWTGYLAYRRL
                     SDQSFHVIVLLLLFFAGVMLIATG"
     gene            7541..8749
                     /locus_tag="VF226_20595"
     CDS             7541..8749
                     /locus_tag="VF226_20595"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEX7076444.1"
                     /translation="MTEPENGKPVAEPSLESTGSEGRGVDELAYRIRQQGLLAEFGVL
                     ALRDQSLDALLSMAAERSAQGLNAEFSKVLHVLPDQSGLRIVAACGWSDDIIGSVIGA
                     DTGSPAGYALMTGRPVISNHLENEPRFSTPDFMVKHGIKRAINVILQGQGPPFGVLEV
                     DSRGPGEFSERDLAFLQGAANLLGMAIEQRRYEEQLKKLLEHQQSLLKEVNHRVKNSL
                     QLAASVLRLQAANAKDEAVRQVLGDAQARVMAIARAHERLYKTTEFTQIDICAYLQEV
                     CADLDGGELAFEGPEGIRIATDRAIPLAMLVSELITNSMKHAYPGREHGPVYVRVAPA
                     ADNMLRLSVSDEGVGLPEGFDPETTKGLGMRLVRAFAQRLDATLEFHRMAPGTEVVLH
                     LARDEPSGSG"
     gene            complement(8822..9610)
                     /locus_tag="VF226_20600"
     CDS             complement(8822..9610)
                     /locus_tag="VF226_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX7076445.1"
                     /translation="MRKNAIRKTLAEGGRVVNAWCSIPSSYSAELMAHQGWDSVTIDM
                     QHGPVDYQVAFTMLQAISTTNAVPMARIPWNDPAIIMKLLDAGAYGLICPMINSKAEA
                     EAFVGAARYAPQGFRSFGPNRAVQYGGADYWQHANEEVLLLAMIETTKALENLDEILS
                     VPGLDGVYIGPSDLSLSMGRTPTLDPTDSDVLAAMTTILTRTRAKGLIAGAHTDSAKT
                     ALKRFNEGFQLCTILNDARLLAMAAQAAVREAKGEAPAQAAKTY"
     gene            complement(9625..10605)
                     /locus_tag="VF226_20605"
     CDS             complement(9625..10605)
                     /locus_tag="VF226_20605"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX7076446.1"
                     /translation="MKHKVICIQPIHPEGMRILHAREDLEIIVPESPEPQHWEQHLTD
                     AEAIVVRISRIERWMIEKAPRLRIISRHGVGVDNIDVEAATERGVVVATIGTANAPSV
                     VEHTLAMMFALAKRLREFDRAVRAGDYMIKMNLDAIDLAGRTILIVGLGRIGSRLAIA
                     CNALGLRCIGIDPAFTADEIRAMGCEPASSLHDALPLADFVSLHCPLQADTRNLIGAR
                     ELALMKPTAYLINCARGGIVDEAALLAALEAGRLNGAGLDVQVTEPPRADDPLLKSDK
                     VILTPHAAATTAEGVVRVSKIVAQNVLDHFDGRLPAAHIFNLEALSRAKS"
     gene            complement(10602..11300)
                     /locus_tag="VF226_20610"
     CDS             complement(10602..11300)
                     /locus_tag="VF226_20610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX7076447.1"
                     /translation="MIDPDLLEQLKTYDTPTICNALEVIDSSRRLTGFTTKPLVAPFP
                     DLPPMVGYARTATIRSTHLHELDAKAARAQRIGYYEYVASQPGPQIVVIQDLDGPDCG
                     FGAFWGEVQSTVHKALGCVGVITDGCIRDIPQWAPGLNVLAGSIKPSHAHVHLADFGK
                     QVRVAGMVVRSGDLIHADRHGAVVIPHALAEKVPAACELMARKEAVILEMARKPGFTV
                     EMLKEALAKQDEIH"
     gene            complement(11504..12592)
                     /locus_tag="VF226_20615"
     CDS             complement(11504..12592)
                     /locus_tag="VF226_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEX7076448.1"
                     /translation="MVTVAAENLRELVRDIFIAAGCSREEGERLGKSLVSANLAGHDS
                     HGVVRVPRYLQWMAEGDFIADQKVEIVRETPVLAVVDGKYGFGQTAAPQAVEIGIAKC
                     RAMGLSAVALRNSGHVGRVGEWAEMAAEAGLVSIHFVNAASSLLVAPFGGVDRRLSTA
                     PYAIGVPRPGEDPLILDFATSVVAEGKVLVASQGGKKLPDNALVGPDGTISGEPALLY
                     GPLEPNGPRDYRKGQGAIRAFGEHKGSGLAFMVELLGGSLTGTGAAQEGRRWANGMLS
                     IYIDPKQLDPEEFFPADVKRYVAFFKSSRPIEAGGEVLVPGEPEIRTRRKRLAEGIPL
                     PEDTWARIVAAARSVGLDEARIERAGAR"
     gene            12796..13977
                     /locus_tag="VF226_20620"
     CDS             12796..13977
                     /locus_tag="VF226_20620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7076449.1"
                     /translation="MDFALPKDLEALGERVLTFIKEEIIPFETDPRWTAHGPTEELRR
                     ELNDRARRAGLLSVHVPKEYGGLGLDHVGKSVVLEAAGYSMLGPTALHCQAPDEGNIH
                     LLEQVANPEQKERYLRPLATAEVRSCFAMTEPHPGAGSDPGMLATRAEKDGNGFVIRG
                     KKWLITGAEGAAFAIIMARTGDGPNDATMFIADLPNPAFKITRTLDTMDSSFIGGHAV
                     VEIEDLRLAGDAVLGEIGQGFRYAQVRLAPARLTHCMRWLGSAQRAHDIASEYARTRQ
                     AFGQRLGAHEGVSFMLADNVMDMHVSRLAIRHCAWLLDQGNRASSESSMAKVICSEAI
                     MRVVDRSLQILGGTGLTRDTVVERIFRDVRAFRIYDGPSEVHRWALGRRVVERKWSNK
                     G"
     gene            complement(13993..14388)
                     /locus_tag="VF226_20625"
     CDS             complement(13993..14388)
                     /locus_tag="VF226_20625"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEX7076450.1"
                     /translation="MTKKYALVAHDARKPLMVDWVGRNLDIFRQLDLIATGTTGKVLL
                     EHYPELRLERLESGPRGGDQQVGALIVAKAIDVLFFFIDPLTPMPHDVDVKALLRLSI
                     LYDVPCACSVRSADIILDSVRKSRAAASA"
     gene            14803..15711
                     /locus_tag="VF226_20630"
     CDS             14803..15711
                     /locus_tag="VF226_20630"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7076451.1"
                     /translation="MPKLASRAQLPEAGEIFLDHVAWMVPDIAKAATVFEQLGFPLTP
                     YAVHGNRDPVTGVLDPVGTANRLAILERGYLEILTAVDGLDTPSTRHMKKAIDHHTGV
                     HLLAFSVPDATEAAREIAARSVELQPTVHLRRTVETDSGEQAEASFTVVRAAFEQFPE
                     VRVQVLTHHTAEHVWQRRFLSRESGLVGLLEAAIVTANPHEAALRFTAFLGRPVEKDG
                     SSLLIRLDRGALRIVDARGAAERYGRISRPPMPAVCAITLTSSNLDQTRYFFLSQGLR
                     PGAINPGHLLIDESEALGVHLVVVPD"
     gene            complement(15912..18083)
                     /locus_tag="VF226_20635"
     CDS             complement(15912..18083)
                     /locus_tag="VF226_20635"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX7076452.1"
                     /translation="MNLALWVIIAAGLISIIYAGVTIKSVMSADPGNQKMQEIAGAIR
                     EGASAYLNRQYTTIAIAGVVIFILALVLLGVWTAVGFAIGAILSGAAGYIGMNVSVRA
                     NVRTAQAATVSLASGLDIAFKAGAVTGMLVAGLALLGVAVYYTFLVVGLGFGTADRVV
                     VDSLVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYAVTVVATMVLAAIFFLGQPNLTSLMIYPLAIGAACLITSI
                     VGTYFVKLGANNSIMGALYRGFIASAVLSIAGLFVATQYVLGTWGEVGSAAGQVISGT
                     DLFICGLVGLAVTGLIVWITEYYTGIGYRPVRSISQASVTGHGTNVIQGLAISLEATA
                     LPTLVIVAGIIITYNLAGLFGTAIATTTMLGLAGMVVALDAFGPVTDNAGGIAEMSGL
                     PSEVRRSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYSYDLQYFISEANKAGSA
                     AYQYFQGVQPDFQLSNPYVVVGLLIGGLIPFLFGGIAMTAVGRAGGAIVLEVRRQFRE
                     KPGIMKGTERPDYAAAVDLLTRAAIKEMVIPSLLPVLSPIVLFLVIYLVAGGGAAGKS
                     AAFSAVGAMLLGVIVTGLFVAISMTSGGGAWDNAKKSFEDGFVDKDGTKHIKGSEAHK
                     ASVTGDTVGDPYKDTAGPAVNPLIKITNIVALLLLAVLAHW"
     gene            complement(18309..19298)
                     /gene="thiL"
                     /locus_tag="VF226_20640"
     CDS             complement(18309..19298)
                     /gene="thiL"
                     /locus_tag="VF226_20640"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX7076453.1"
                     /translation="MSDNDGTAGEDALIRSYFAPLAAGFPGAFGLEEDCAALTPEPGH
                     DLVLKTDAIAEGVHFRATDRPEDIAWKAVAVNVSDLAAKAARPVGYLLSLAFPRMPDR
                     SWLEQFSGGLKAAQEHFGLVLMGGDTDRRSNAPLSVTIMAIGSVPSGRMVRRGTAQAG
                     DFVYVSGTLGDSAIGLSIPAGLTAADRAYLEKRYLRPEPRLALRDALLGYANAAMDIS
                     DGLVKDLGRLCRASGLAAEIEAALVPVSEAARRAIELQAELRLLPLTGGDDYEILAAV
                     SPERATDFEASASAAGVPVTRIGSLQAGSGVQVIGPDGKPIELHRLGYDHFQR"
     gene            complement(19457..19954)
                     /gene="nusB"
                     /locus_tag="VF226_20645"
     CDS             complement(19457..19954)
                     /gene="nusB"
                     /locus_tag="VF226_20645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7076454.1"
                     /translation="MTSEDYTVKRNPRSAARVAAVQGLYQMDMAQTDLNAVIAEFQDL
                     RFRQAEPGDAIVGADPTFFAELLRGVVRRQRDIDPLVNQQLAAGWNLGRVDSILRAIL
                     RSGVYELLERPDVPARVVINEYIDIAHAFFSEDEPKVVNGVLDKIARRLRPGEFPDRP
                     RGGAR"
     gene            complement(19951..20475)
                     /gene="ribH"
                     /locus_tag="VF226_20650"
     CDS             complement(19951..20475)
                     /gene="ribH"
                     /locus_tag="VF226_20650"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7076455.1"
                     /translation="MVGRGSRTTVSTAGGATAGRVRCRVLIIEGRFYDHICDALLEGA
                     VAELDAAGAAYDRITVPGALEIPQVLAQAVSAGLIPATAPTSWYCGAIALGCVIRGET
                     AHYDIVCNNANHWLMDVAIRHNVPVGNGILTVDTEAQALERAKGGRDGKGGAAARACL
                     QLIEIARQFQGQGA"
     gene            complement(20483..21643)
                     /gene="ribB"
                     /locus_tag="VF226_20655"
     CDS             complement(20483..21643)
                     /gene="ribB"
                     /locus_tag="VF226_20655"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX7076456.1"
                     /translation="MNQSLDTVQYSEGQGVLSTTPELLDDLRNGRMVILVDAENRENE
                     GDLIIPAQMATPDAINFMAKHGRGLICLALTKARAEQLRLESMVRANASRTGTAFTVS
                     IEAREGITTGISAHDRARTIATAIDPTKDHDDIVSPGHVFPLVAREGGVLVRAGHTEA
                     SVDLARLAGLYPAAVICEIMNEDGTMARMPDLVPFAQRHGLKIGTIEDLIAFRLRNDR
                     IVKPVARRQVESAFGGSFELYVYETTVEPVEHLALVKGDLTKPGPVLVRVHAVNMLSD
                     MLGIGTSTEAAGDSLIAQSMRAIAQEGRGVLVLIRDLRPKSVSNWIGERSGLAPRKTE
                     GEERRLVEIGVGSQILRDLGVRDMVLLTNSPASVYVGLEGYGLKIVEQRRIG"
     gene            complement(21640..22233)
                     /locus_tag="VF226_20660"
     CDS             complement(21640..22233)
                     /locus_tag="VF226_20660"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7076457.1"
                     /translation="MFTGIVTDIGEIAARDGGRFSIRSSYAADSIKLGASIACDGCCL
                     TVTAVEPAGQGSVFSVDTSNETRSKTTIESWQPGRRINLERSLAAGDELGGHIVTGHV
                     DGVARIVDIQPDGESRRFTFEAPEHLALYIAPKGSVALDGTSLTVNEVAGNRFGVNLI
                     PHTLTVTTWGAKTAGHLVNLEIDLFARYVARLLEFRR"
     gene            complement(22294..23421)
                     /gene="ribD"
                     /locus_tag="VF226_20665"
     CDS             complement(22294..23421)
                     /gene="ribD"
                     /locus_tag="VF226_20665"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX7076458.1"
                     /translation="MARTIASDTQSERDGRFMDIALRLARRGLGNTAPNPAVGAVIVD
                     EASGEVIARGWTQPGGRPHAETEALRRAGARARGATMYVSLEPCSHHGGTPPCADALV
                     EAGLARVVVGIEDPDARVAGRGLDRLRAAGISVTRGVRSEEADWVARGHILRVSERRP
                     FIQLKLALSADGSVPRGTEGKPVWVTGPEARAQGHLLRAMSDAILVGSRTVRDDDPEL
                     TCRLPGLADRSPVRVVLARDLDIPIESKLIRSARDVPVMVFCGADADARKRAALEKHG
                     CEVIPVRVVEGMLWLPAVTEGLAARSITRLLVEGGPAVWRSFARAGFVDEAVVFQARG
                     RAGIVRRPPAAYLPGVDLMLTAQREFGPDDMMIFRRPNRPA"
     gene            complement(23399..23893)
                     /gene="nrdR"
                     /locus_tag="VF226_20670"
     CDS             complement(23399..23893)
                     /gene="nrdR"
                     /locus_tag="VF226_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7076459.1"
                     /translation="MRCPFCGSGDTQVKDSRPTEEGAAIRRRRVCPDCGGRFTTFERV
                     QLREITVIKRSGRKVPFDRDKLARSIEIALRKRPVEAERIERMVSGIVRQLESTGESE
                     VTSSAIGETVCEALRGLDPVAYVRFASVYRDFREASDFEEVLTEIAKGNGAGQESHGQ
                     NDRE"
     gene            complement(24045..25349)
                     /gene="glyA"
                     /locus_tag="VF226_20675"
     CDS             complement(24045..25349)
                     /gene="glyA"
                     /locus_tag="VF226_20675"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7076460.1"
                     /translation="MSTASPGRPNASPGFFTEALAERDPELFGAVANELERQQNEIEL
                     IASENIVSRAVLEAAGSVLTNKYAEGYPGRRYYGGCQFVDVAEQLAIDRAKRLFGCAF
                     ANVQPHSGSQANQGVFMALAKPGDTILGLSLAAGGHLTHGSPVNMSGKWFKPVHYGVR
                     RQDHRIDHDEVRSLAKEHRPRIIIAGGSAYPRTIDFAAFREIADELGAYLMVDMAHFA
                     GLVAGGAHPNPVPHAHVVTTTTHKTLRGPRGGMILTNDPEIAKKVNSAIFPGLQGGPL
                     MHIIAAKAVAFGEALRPEFKAYAKAVVENAAALGDVLKASGYDLVSGGTDTHLMLVDL
                     RPKKLTGNISERALGRAHITCNKNGVPFDPEKPTVTSGIRLGSPAGTTRGFGIDEFKA
                     IGRMIADVLDGLAKNGEEGNAAVEARVREEAIELCRKFPIYN"
     gene            complement(25514..26743)
                     /gene="hemA"
                     /locus_tag="VF226_20680"
     CDS             complement(25514..26743)
                     /gene="hemA"
                     /locus_tag="VF226_20680"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEX7076461.1"
                     /translation="MDYTARFQNVLEEIQAEGRYRVFADLRRNCGAFPAASYYGSGSK
                     QAITVWCSNDYLGMGQHPLVLSAMHEALDTTGAGSGGTRNISGTTHYHVELEAELAEL
                     HRKEAALLFTSAYIANDATLSTLVKLLPGAVIFSDEKNHASMIEGIRRGGGPKRIWRH
                     NDLADLEAHLKEFDRETPKIIAFESVYSMDGTIAPIAAICDLADRYGALTYLDEVHAV
                     GMYGPRGGGIAERDGVLDRVDIINATLAKGFGVMGGYIAASRPCIDAIRSCAPGFIFT
                     TSLAPVIVAGALASVRHLKASAVERERHQERARTLKRRLTAAGLPVLDNPSHIVPVMV
                     GDPVLCKKISDALLARFGIYVQPINYPTVPRGTERLRFTPTPLHSDRDIDHLVSALTV
                     LWGEFGLELGPVRVAAE"
     gene            complement(26922..28208)
                     /locus_tag="VF226_20685"
     CDS             complement(26922..28208)
                     /locus_tag="VF226_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7076462.1"
                     /translation="MVAVRAFSGRRLANLIGFAAAGITASIVTGTPAAAYDSASWNPF
                     ADTVSSYTQPYDRAFIREWEANPPRGYPTVSPANIGPMKAAIKRYAAIVEQGGWKPVP
                     NVRLQAGMTDRAVRILRERLLMSGDLREGSNYPDYFDYYLERAVKRFQASNGLAPTGI
                     VDHRTIGALNVPAAARLRQLRTNLDRVSELARTAGKRYVLVNIPAAQIEAIENDRVVS
                     RHAGVVGKIDRPTPVLRSTIRELNFNPIWTLPPTVIAKDLIPKGRELARSNKDVLQVY
                     GIDAYGPDGRKLDSSKIDWSSEMVHHLTYRQQPGKDNPLGFLKINFHNGYSVYLHDTP
                     SESIFGRNFRAATSGCVRVQGIEQLAAWLLHDNGWTLAHVMALKESGERKDVSLKKPI
                     PIYMVYITAWATEDGVVQFRRDLYQKDGVGAVAAAY"
     gene            complement(28543..29049)
                     /locus_tag="VF226_20690"
     CDS             complement(28543..29049)
                     /locus_tag="VF226_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX7076463.1"
                     /translation="MRFLHVEAQPVPIAKNDLEGEYLEALRLIERLHRLLLDVIKDEF
                     DRNGGCDLNPVQALLLHNLGDEELTAGELKTRGYYLGSNVSYNLKKLVELGYIHYKRS
                     DHDRRSVRVRLTSKGEAICETVRRLYRRQLRSLGSVGEVGFDDLQDLNRVLMRLERFW
                     SDQIRYRL"
     gene            complement(29183..30286)
                     /locus_tag="VF226_20695"
     CDS             complement(29183..30286)
                     /locus_tag="VF226_20695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7076464.1"
                     /translation="MPEGSAKESCEELTIAVEGAAAIVTLDRPQSGNALSGAIRSRIA
                     ESIPRFARDPGVYALIMRSGTQGAFSVGSDLRELGSAAKEQPERARQLLADTYRLSWI
                     LECFSKPTISLIDGLIMGSAAGLTAYGTHRVAGETFSFALPQTGFGFFADAGLSWVCA
                     RMPDEIGVYLGLTGRRIGRADAYRLGLVTHCIPASRFSEIEAGLRSADPVDPLLDDRH
                     EEPGPGEIESYREVLQRCFAAETVEEICSRLRAEAQRGGAAGGWCEDVLQDLSRRSPT
                     ALKITLRHIREAAFRDLRQTLMIDCRLACRLVGSHDFREGVRVLLANQDRPRWIPEHL
                     SEVSEATVDRIFAFQPGRELILPTRQEMQAARF"
     gene            30458..31621
                     /locus_tag="VF226_20700"
     CDS             30458..31621
                     /locus_tag="VF226_20700"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX7076465.1"
                     /translation="MPLKVLATGARQAGFALFAAALLCTHPAWSKGAASSGKPAEAQA
                     PSVPKNAAGVGTASEGSKPAEADASEANPAPDVWSDQEIAEAKAYCDQILHGVEAVIE
                     PADPIKEGECGAPAPVRLLSIGRNPEVTLSPPVTVTCDLVVRLAEWLKDDLQPLARKH
                     LGGEIVRVNTMSSYSCRNAYGRKKTKLSEHGRANAVDLGSFVTGDGVEADLLKGWGLT
                     ARDVQKIVARSKAAKTAALMANGQSGDPKPAPERNGKGQPALMVASMASMGSMVPLPI
                     RKPPADVAQWQVQLKLRSGKGSIRPSPELPTRRPQAVAMNEASHLGGPEAEEADVAPE
                     PFAPDDQVALFLRAAHTAACRRFGTVLGPEANEAHRNHFHLDMAKRKHSNYCE"
     gene            31788..32774
                     /gene="hemB"
                     /locus_tag="VF226_20705"
     CDS             31788..32774
                     /gene="hemB"
                     /locus_tag="VF226_20705"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7076466.1"
                     /translation="MFPAVRMRRNRRADWARRLVAENALTASDLIWPMFVVEGTNTRV
                     PVESMPGVERLSVDLVVAAAEEALSLGIPAIALFPYTDPALRTEDAREAFNPENLVCR
                     ATRAVREAGLNIGVVLDVALDPYTSHGHDGLMQGNTILNDETLEALVRQSLVQVEAGC
                     DILAPSDMMDGRIGAIRQALESAGHQSTQIMAYAAKYASAFYGPFRDAVGSTGALKGD
                     KRTYQMDPANTDEALREVALDLAEGADMVMVKPGMPYLDVLRRVRDTFGVPTFAYQVS
                     GEYAMLMAASERGWLDGDKVIMESLIGFKRAGAAGILTYFAPRAARLLASTR"
     gene            33119..33859
                     /locus_tag="VF226_20710"
     CDS             33119..33859
                     /locus_tag="VF226_20710"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEX7076467.1"
                     /translation="MTEQQKNFPEFYVTAPQPCPYLPGKLERKLFTHLTHDKPAALID
                     NLLKGGFRRSQNIAYMPYCEGCQSCVSVRVIVDEFKLNRSFRRVLDANRDLAVRRVPP
                     RPTAEQYALFRDYIDARHADGGMADMTVLDYAMMVEDSVVDTFLTEYRVRTDSGLDSA
                     TAGTLTAVALCDRLSDGVSMVYSFYDTEMPRRSLGTHMILEHIEYTRTLGLPYLYLGY
                     WIEGSRKMSYKTRFQPQERLTSSGWVRV"
BASE COUNT         6027 a        10819 c        11164 g         6156 t
ORIGIN      
        1 cggaacgaaa cagcaagatc cgagtgcaac gcattcgttg gcaaggctcg aaggcggcgt
       61 gaagccgaca gcttgcgcgg ctcactctgc tgccttcggc acctccggca cttcgcaacc
      121 cgtcacacaa cagcggccgg gttgtttgga gacgcgctgc ccaacgtcca acacaccgcg
      181 gtccagcagc cgctcgacga gctctcgctt gtccatcagc ggataattgc gccagatctc
      241 gcgcctcatg gcctcgggtg agccaccgcc gtgcagcgaa atcagcgagt accagccgcc
      301 ctgatgggag gccgtcagat cctcgatgag ccgggcgact tccatgcgtt tttccgccgg
      361 gatgtcgggc cggcctgcga gaacggcgct gagggaagcc ttggtttccg gattgtgatc
      421 ctcgtccggc cccggtagcg ccacgacgag gcccccggag acgtaatggg caaggcggtg
      481 catgtcgtag atcttggtcg cgagcagcag cttgccgatg ttcgagaaca ccgcgtccgg
      541 catgatcgag ccggcaggat ccttcatgcc gtagaccgac gcggccaccc cgcacgcgaa
      601 gaagctttcc gtgatggtga tgagctccac catcgcatcg cgaatgtggc catgccgatc
      661 gggatcgagc ccattcgatt cgagcatcaa cacgcctgcg ccgatcagga gatcgccgaa
      721 gccggcgcgg gcgccgatgc aggaatggcg gtggtgcgtg gcgtaagagg tcgtgaggaa
      781 gccgccttcc tcgtgctcgc cggccaaaaa cacccgatcc catggcacga acacgtcatc
      841 gaacatgacg acgccggtcg actggccata cttggctgag aacttcgccg cggcctctcc
      901 cggcctcccg gctgggcggg cgacgatggt cacgccttcg gcgtcaacgg gaacggcgca
      961 acagacggcg aagtcgcggt cctcggccgt catcgtgcgg cagggcatga cgagaaactc
     1021 gtgcacgtaa ggtgccccgg tgacgattgc cttcgtgccg cgaatgacga tgccgtccgg
     1081 ccggcgctcc ttgatgtgca catagacgtc cgggttgctc tgcgcccccg ggcgcttcga
     1141 gcggtcaccc ttcgcgtcgg tcatcgcaac ggcgacggtc agatcctcgt cctgtatgcg
     1201 gtggaggtag gccagaaagc gctgatggta gtccgtgccg tggttgagat ccgtccgccg
     1261 cgtcgcctgg aaaaccgcat tgagcgagtc gtggctcaga taccgcatcg cgcagccgga
     1321 ttcgcggcac acgagtcgca cggcctccag cttgtaaagc aggtcctgcg aggtctcgtt
     1381 gatgtgcagc atccggttga cgaccttccc cgaagtgcct tggcgcgcca gcatcagcgg
     1441 tttgtgctgc tccttgagcg caaagtcgta ggtgatgccg atggcgttga tgccgggggc
     1501 aagccgcggc tcgtcggcga cactttcgac ccgctcgcca ttgacgaaca cgcgcggtct
     1561 gtagcggcgc aggctctcgc gatattcggc tgccgacatg agcatggggc gtcctcctcc
     1621 gggcgggcag tcgcgaagtg tttcatcagt gctagagtac tgcaaccacc ggcagccggc
     1681 tcaaggacct ttcatgcgat ttgcccccga gacgagcgta ctattgctca tcgactttca
     1741 ggcccgcctg atgcccgtca tctgcgatgg tgacatcgcc gtggccaatg cccggcgcct
     1801 cgccgaggct gccaggttgc tcggcgtgcc gatccgcgcc acggagcaga atccggctgg
     1861 actcggtcgc aacgtgggcc agcttgctcc atttgcagag cggacgctca ccaagcgcta
     1921 tttcgatgca acccgagagg cagaatgggc agggttcatt cccgagggca ggcctcacat
     1981 cgttgtcgcc ggctgcgaga cccacgtctg tgtgatgcaa acggttctcg gaatgctcga
     2041 aaaggggctg caggttcggc tggtgcgcga tgccgtcggc tcgcggaccc cggaaaaccg
     2101 ggaggcggcg ctgcatcggg ccgagcgcca cggcgccgag ctggtcacta ccgagatggt
     2161 cgtcttcgag tggcttgcga cttcggacca tccgcggttc cgggaggccc ttgctctcgt
     2221 gaagtaacta tggcctgcga tcacgtgttg gcttagtctc agtgatgccg aaattccgga
     2281 ctaagccgat ccggcattgt tggggggcgg aaaagtggtt gcgaatgaag gcgtactcat
     2341 tatcggtgaa gacacgttcg ttaccggtaa gatccgcaat tgccgccgga ttgagatttt
     2401 cggccgagtc catggcgatg tgacggcgga taccgtccat atccacgagg gtggacggtt
     2461 ggacggaaag gcccgagccg agaacgccga tgtgcacggc gtattgcagg gcaacgtcac
     2521 tgtacggaat ttgataaaca tccgcagctc tggctccgtc agcggcaacg tccagtacgg
     2581 ccagcttgcg atggaggtcg ggggcagcct cagcgccgag gtgcgcaacg tacccccatc
     2641 cataggcggc gacctggacc tttccgtcta ccgcgggcgg tccgtcggcg tcacactcga
     2701 ggacctgacc gccatcgatc cggacgacga ggcccaggat ctgatctacg gcgtctccaa
     2761 cgcgaagaac ggcttcgtcg cattgaccca tgcgccggcg gaagcggtga caagcttcac
     2821 ccaggcggac ctcgaggccg gcaaggtgat tttccggcat gatggatccg caacggattg
     2881 ggcgagcttc gacgtggtgg tgaccgatgc gtccggcgcc aattccggca gtccccagac
     2941 ggtgaaggtc acggtccaca gccgcagcgg atgaaatctc gcgtgcgccc gaatccatta
     3001 ttcggggcct gaactcgtct acggctgcgg cttggacgct gatttcatgc cataggtgtg
     3061 ctcgggcgcc ggaaaggacc gatcacggac gctggcggcg tagtctgcga ttgcgcgatc
     3121 gatcgccgcg ccgatctggc cgaattgcct gacgaatttc ggcacgcggg ccgacattcc
     3181 aagcatgtcg ttcatcacca ggatctgacc gtcgcacgcc gcggaagcgc cgatgccgat
     3241 ggtcgggacg gggatggtat tggtcagttt cgcggccaga ggctctgcca cagcctccac
     3301 gacgacggcg aaggcgcctg catcgctaac ggcaatcgcg tcggcttcaa tctcgcgcca
     3361 ctgttcctcg gttcgaccct gggcgcggaa gcccccgagc gtgttgatcg cctgtggcgt
     3421 gagcccgaca tgacccatca ccggtatccc gcgctcgacg agaaagcgga tcgtctcggc
     3481 catctggcgg ccaccctcga gcttgaccgc accgcatccc gtctccttca ggatgcgggc
     3541 ggcgttgcgg aaggccattt ccgggctttc ctcgtatgag ccgaacggca ggtcgacgac
     3601 gaccagcgcg cgcctggtgc cgcgcacgac agcgctgccg tgcatgatca tcagctcgag
     3661 cggaaccggc acggtggtgt cgaacccgtg cacgaccatg ccgacgctgt cgccgaccag
     3721 caggaaatcc acgtggctat ctgcaattgc ggcgatgttg gcgctgtagg cggtcagcga
     3781 gacgatcggc gtgccgccct tgcgagcgcg gatatcgaca gatgtggttc ttttgacgac
     3841 ggtttggctg gacacggctg cctccagtgc ggaacgtgct tagctcgctc aactgtcgac
     3901 tacctaccca tcctggtccg caaggccaag gtggtgctgt tctccgtcgc gtcctggggt
     3961 atagaacggg gaatctgatc cgcaggagaa ttccacgtgc ttctgggctg cattgccgat
     4021 gatctgaccg gcgcgacaga cctcgcgctc atgctgaccc gcaacggcat gcgcacggtg
     4081 caggtcatgg gcgtgccggg acccgaggcg ccgctcgaca atgccgatgc cgtagtggtg
     4141 gcgctgaaat cgcgcaccaa cccggtcgat gaagccgtcc gctggtcgct ggcgagcgcg
     4201 gacgcgctac tcgctcacgg cgcaaggcag ctttacttca agtactgctc cacgttcgat
     4261 tcgacggaga agggcaacat cgggcccgtc gccgaagcgc tgctcgagaa ggtgaagggc
     4321 gaaatcgcgc tcgtctgccc ggcattcccg gccaacaagc gcaccgtcta catggggcat
     4381 ctgttcgtcg gcacggttcc tttgcatgaa agcccgatga aggatcaccc actgacgccg
     4441 atgcgggact ccaatctcgt ccgcgtgctg cagcggcaga cgcggctgaa cgtcgggctc
     4501 gttccttatg acgtggtcga tcaaggggcg gatgcgaccc gtgcggcttt caatgaggct
     4561 gcaaaggagg gaacgcgctt cgcagtcgtc gacgcgatca gcgatcgcca tttgattgcg
     4621 atgggcaccg cggcggccga tcatgtgctg atcaccggcg gctctggtgt ggccatgggc
     4681 cttccggaca acttcgcccg caaggggctg ctcgagaaaa cggagccgat gggacacatc
     4741 tcggcgccgg cggggcgctc gttgatcatc gcggggagct gctcggaagc gacgcgcggc
     4801 caggtgcgtg ctgcggaagc cgccgggttg cctgccttca aggtcgaccc tctcgccatc
     4861 gcacgcggca agctcgctgc tccggacatg gtctcctggg cattgaagca gccgggggat
     4921 cgacccgttt tgatctactc gagcgatgat cctgccaacg tacgcgccgt gcagacggag
     4981 ctcggacgtg aggccgccgg tgcgctcgtc gagcgggagc ttgcggcagc cgcaaaggag
     5041 ctcgtcgaca aaggcttcac acgcctcatc gtagcgggcg gcgagacctc cggcgcggtc
     5101 gtcggcgctc ttggcgtcaa ggccatcgag attggcccag agatcgaccc gggcgtccca
     5161 tggacgcgca gcatcggcgg ccgcgggctg gtgctggcgc tgaaatccgg taatttcgga
     5221 gcgccggact tttttctcaa ggcctggaat cttatcgaat gagcgcagaa tcgaagcttc
     5281 gcgagcggat ctgtgccgcc ggcgcttcgc tcttcgatcg cgggctgacg cacgggtcga
     5341 cgggcaacat ctccatccgg ctcgaggatg gcggctggct gatgaccccg acgggctcct
     5401 cgctcggcac gctcgatccg gcgagattgt ccaagctcga tccggcgggc cagctcgttt
     5461 caggcgaccc gccgacgaag gaagcgtttc tgcacaccac gatgtacagc gagcgcccca
     5521 aggccggcgc ggtcgtgcac ctgcattcga cctattcggt ggccgtgagc tgtcttgcgg
     5581 acatcgaccc cagcaactgc cttccgccaa tcaccgccta ctacgtgatg cggatcggca
     5641 cgttgccgct ggtgccttat ttcccgcccg gagatcacag cctcgccgag gcggtcggaa
     5701 aactcgctgg ccggcatcat gccgtcttgc ttgcgaacca cgggcccgtt gtcgccggat
     5761 cgagcttgtc ggcggccatg gatgccatcg aggagctgga ggaaacggcg cggctatttc
     5821 tgctgctccg cgatcgcccg gtccgtactt tgagcgaagc tcaggttgcc gagttgcgcc
     5881 ggcgctttcc aaactagggc tagacctgga aactcatcaa atctgaaaac caaggggaag
     5941 aacatggcga acaagatcga tctcgaaaac cgcgtggccg tcattacggg cggcgcgcag
     6001 gggttcgggc gggcgtcggc ggagcggttc atcgcatcgg gagcgaaggt ggcgatctgg
     6061 gatcgcgatt tcgcccttgc ggaaaagacc gcagccgagc tcggcagcaa tgtgaaggcg
     6121 ttcaattgcg acgtcactga tgcgaagtct gtcgacgcgg cccgcgatgc gacgctctct
     6181 gccttcggca agatcgatat tctcgtgaac aacgccggta tcgcagggcc cacggcgaag
     6241 gtatgggaat attcgctcga agagctgcag atgaccatgc gggtcaatct cgagggtccg
     6301 tggctctgct gcaaggcaat cgtgcccggg atgattgcgc agaactacgg gcgcatcgtg
     6361 aatatcgcgt cgattgccgg caaggaaggt aatccgaacg ccagcgccta ttccgcctcg
     6421 aaagcggcct tgatcgcgct gacgaaatcg ctcgcaaaag aagtggcgac ctacgacatt
     6481 tctgtgaatg ccattgcacc cgcggcagcg cgcaccgcga tcctcaatca ggtcacggaa
     6541 gagcacatca actacatgct ctccaagatt ccgcggggcc ggctggtcac ggtggaggaa
     6601 gttgccgccc tcgtcgcctg ggccgcgtcg tccgactgct cgttcacgac cggtcaggtt
     6661 ctggatatct ccggcggccg cgcaacctac tgatcgtctc cggaacggcg gcgccgagtg
     6721 gcaacccaaa cgatattcat cctgctcggc gcattcgctg gcggtttcgt gaacgggatg
     6781 accggtttcg ggaccggtct gacggcgctc gccttctggc ttcacgtcat cggcccggcg
     6841 ctcgcggcgc cgcttgtgtt gacatgctca gtcatcgcac aattgcagac gctgccgcat
     6901 atctggcgcg agcttgattg gcggcggctg atgcccttca tcttgggcgg cctcgccggg
     6961 gttccgatcg gcacacagct tctgaccgag atcgatccca aggtcttcaa gttcgtcatg
     7021 gggatcgtgc tgatttgcta ttccagcagc atgctcgtgc tgaagccgcg cttcggcgca
     7081 aagtggggtg ggcgtgtcgc cgatggtctt gtcggcttga gcggcggtat tctcggcggg
     7141 tttgctggcc tttcgggcgt gcttccgacg atctgggctg ccatccgcgg atggagcaag
     7201 cccgagcgcc gtggggtatt tcaggctttc aacctcacga tactcggtac ggcgctgatc
     7261 gcatatgcgt ttgccgggct acttaccgcc gaggtcggca gcctgatcct gaccgccgtt
     7321 ccagggacgc tgattggaag ctggacagga tatctcgctt accggcggct cagcgaccag
     7381 agctttcatg tcattgtcct gcttcttcta tttttcgctg gcgtaatgct gatcgcgacg
     7441 ggttgatgcg cttgccgggg acgcccgcaa accccacgta gggggaattg cgaaagccgc
     7501 agtctgcggt cgtgccctag tgtttcttga gtaggcgtgc ttgacagagc ctgagaacgg
     7561 caaaccggtc gcggagccca gcctcgaatc caccggatca gagggacggg gcgtcgacga
     7621 gctggcctat cgcatccggc agcaggggct gctcgctgaa ttcggcgtgc tcgcgttgcg
     7681 ggaccaatcg ctcgatgctc tgttgagcat ggcagcggag agatcagcac agggcttgaa
     7741 cgccgagttc tccaaagttc ttcacgtcct gccagaccag agcgggctca gaatcgtggc
     7801 cgcttgtgga tggagcgacg acattatcgg ctccgtcatc ggtgcggaca ccggctcgcc
     7861 cgcaggttat gcgctcatga cgggcaggcc cgtcatctcc aatcaccttg agaatgaacc
     7921 tcgcttcagc acgccggatt tcatggtgaa gcatggcatc aagcgcgcga tcaatgtcat
     7981 cctccaaggc cagggaccgc cattcggggt gctggaggtc gacagccgcg gccccggcga
     8041 gttcagcgaa cgtgatctcg ccttcctgca aggggcagcc aacctgctcg gcatggccat
     8101 cgagcagcgg cgatacgaag agcagctcaa aaagctcctc gagcatcagc aaagcctgct
     8161 gaaggaggtc aatcaccgcg tcaagaacag cctgcagctt gcggccagcg ttctacgtct
     8221 gcaggcggca aacgcgaagg atgaggccgt ccgccaggtt ctgggagatg cgcaggcgag
     8281 ggtgatggcg atcgcccggg cgcacgagcg gctctacaag acaaccgaat tcactcagat
     8341 cgacatttgc gcctacctcc aggaggtctg cgcggatctg gatgggggcg agctcgcatt
     8401 cgaaggtccg gagggcattc gtatcgcgac ggatcgcgct attccgctgg cgatgctcgt
     8461 ctccgagctc atcacgaaca gcatgaagca tgcctatccg ggtcgggagc acggacccgt
     8521 ctacgtgagg gtcgcccccg ctgcggataa catgctgcgg ctatccgttt cagatgaggg
     8581 cgtgggcctg ccggaagggt tcgatccgga gacgaccaag gggctcggca tgcggctcgt
     8641 cagggccttt gcgcagcggc tcgatgccac cctggagttt caccgcatgg ctcccggcac
     8701 cgaagtggtg ctgcatttgg cacgcgacga gccgagcggc agcgggtaaa ggtacctcat
     8761 gtaaacggcc cggcacacct gatgccgagg cgtgacgtgg atcgctcgag cagttccgcc
     8821 ctcaatatgt ttttgccgcc tgagccggtg cctcgccctt ggcttcgcgg acagcggcct
     8881 gcgccgccat cgccagcagg cgcgcgtcgt tcaggatcgt acagagctgg aacccttcgt
     8941 tgaagcgctt gagcgcggtc ttggcgctat ccgtatgtgc gccggcgatc agccccttgg
     9001 cgcgcgtccg cgtgaggatc gtcgtcatgg ctgccagcac gtcggaatcg gtcgggtcaa
     9061 gggtcggcgt gcgccccatc gagagcgaca gatccgaagg accgatatag acgccgtcga
     9121 gacccggtac ggagagaatc tcgtccaggt tctccagcgc tttcgtggtt tcgatcatcg
     9181 ccaacagcag cacttcctcg ttggcgtgct gccagtagtc ggcgccgccg tactgaacgg
     9241 cacggttcgg gccgaaggac cggaagccct gtggcgcgta acgtgccgcg ccgacaaacg
     9301 cctcggcttc ggccttgctg ttgatcatcg ggcagatgag cccgtaagcc ccggcgtcga
     9361 gcagcttcat gatgatggcc ggatcgttcc acgggatgcg cgccatcggc acggcattcg
     9421 tcgtggagat ggcctgcagc atcgtgaagg cgacctgata gtccaccggc ccgtgctgca
     9481 tgtcgatggt gacgctgtcc cagccctgat gcgccatgag ctcggccgaa taggagctcg
     9541 ggatcgagca ccaggcgttc accacgcgac cgccttcggc cagcgttttc cggatggcgt
     9601 tcttgcgcat cgcttcaccc ccactcagct cttggcgcgc gagagcgcct ctagattgaa
     9661 aatgtgtgcg gccggcagac ggccatcgaa atggtcgaga acgttctgcg ccacgatctt
     9721 ggacacgcgc accacgcctt ccgccgtcgt cgcggcagcg tgtggcgtga gaatgacctt
     9781 gtcagatttc agcagcgggt catcggcacg cggcggttcc gtgacctgaa cgtcgaggcc
     9841 ggcgccattc aagcgtcctg cttccagcgc cgccagcaag gctgcctcgt cgacgatgcc
     9901 gccgcgggcg cagttgatga gatacgcggt cggcttcatc agggcgagct cgcgcgcgcc
     9961 gatcaggttg cgtgtatcgg cctgcagcgg gcagtgaagc gagacgaagt cggccagcgg
    10021 caacgcatca tggaggctcg aggcgggctc gcaccccatc gcgcggatct catccgcagt
    10081 gaatgctgga tcgatgccga tgcagcgcag gccgagggca ttacaggcga tggcgaggcg
    10141 gctgccgatg cgccccaggc cgacgatgag gatcgttcgt ccggcgaggt cgatcgcatc
    10201 gaggttcatc ttgatcatgt agtcgcccgc gcgcaccgca cggtcgaact cgcgcaggcg
    10261 tttggcgagc gcgaacatca tggccagcgt gtgctcgacg acggagggcg cgttggcggt
    10321 gccaatggtg gcgacgacaa cgccccgctc agtcgcagcc tcgacgtcga tgttgtcgac
    10381 gccgacaccg tggcgcgaga tgatcctgag ccgcggcgcc ttctcgatca tccagcgctc
    10441 gatccgcgat atgcggacca cgatcgcttc ggcgtcggtc agatgctgct cccaatgctg
    10501 cggctccggg ctctcaggga caatgatctc tagatcctcg cgcgcgtgga ggatcctcat
    10561 gccctccgga tggatcggct ggatgcagat gaccttgtgc ttcaatggat ttcgtcctgt
    10621 ttcgccagcg cttccttgag catttcgacc gtgaagcccg gcttccgcgc catctcgagg
    10681 attacggctt ccttgcgtgc catcagctcg caggccgcag gcaccttctc agcgagcgca
    10741 tgcgggatga cgacggcgcc gtgccgatcg gcgtggatga gatcgccgga gcggaccacc
    10801 atgccggcga cccggacctg cttgccgaag tcggccaggt gaacgtgcgc gtggctcggc
    10861 ttgatggagc cggccagcac gttcagtccc ggcgcccact ggggaatatc ccggatgcag
    10921 ccgtcggtga tgacgccgac gcagcccagc gctttatgca cggtgctctg cacctcgccc
    10981 cagaaagcgc cgaagccgca gtccgggccg tcgaggtcct ggatgacgac gatctgcgga
    11041 ccgggctggg aggcgacata ttcataatac ccaatccgct gcgcacgggc tgccttggcg
    11101 tcgagctcgt ggaggtgggt cgaacggatg gtcgccgtgc gcgcatagcc gaccatgggc
    11161 ggcaggtccg ggaacggtgc cacgagcggc ttcgttgtga agccggtgag gcgtcgtgag
    11221 ctgtcgatga cctccagggc gttgcagatg gtgggtgtgt cgtaggtctt gagttgttcc
    11281 aggagatcag gatcgatcat cgatatgccc tcggcagttg gcgccaatcg tcggtccgtg
    11341 tccgatcact ttttcgcact cccttggtca tcccggcgct cgtcgccggg atccacccag
    11401 cccctggtag cgcctgcgga tggatggata ccggggacaa gccccggtat gacattttgc
    11461 ggtatttccg ctctcctcac ttcacttcga tctcgacctc agcttagcga gccccggccc
    11521 gttcgatcct tgcttcatcg agaccgacgc tgcgggcggc ggcgacgatg cgcgcccatg
    11581 tatcctcggg aagcggaata ccttcggcga gacgcttgcg gcgggtacgg atttccggct
    11641 ctcccggcac cagcacctcg ccgccagcct cgatcgggcg cgagctcttg aagaaggcaa
    11701 catagcgctt gacatcggca gggaagaact cctccggatc gagctgcttg ggatcgatgt
    11761 agatcgacag catgccgttg gcccagcggc ggccttcctg agcggcgcca gtgccggtca
    11821 gtgagccgcc gagcagctcc accatgaacg cgaggccgga gcccttgtgc tcgccgaacg
    11881 cgcgtatcgc gccctggccc ttccggtaat cgcgcggacc gttcggctcg agcggaccat
    11941 agagcagtgc tggttcgccg ctgatcgtgc catcgggtcc gacgagcgca ttgtcgggaa
    12001 gcttcttgcc gccctggctc gcgaccagca ccttgccctc ggcgacgacg gacgtcgcga
    12061 aatcgaggat cagcggatcc tcgcccggcc gcggcacgcc gatcgcgtaa ggcgcggtcg
    12121 acagccggcg gtcgacacca ccgaaaggag cgacgagcag gctggaggct gcgttgacga
    12181 aatggatcga tacgaggcca gcttccgctg ccatttcggc ccactcacca acgcgaccga
    12241 cgtgaccgga gttacgcagt gcgacagcgg aaaggcccat ggcgcggcac ttggcgatgc
    12301 cgatctcgac cgcctgcggc gcagccgtct gaccgaatcc gtacttgcca tcgacgacgg
    12361 cgagaaccgg cgtctcgcgc acgatctcga ccttctggtc ggcgatgaag tcgccttccg
    12421 ccatccattg cagatagcgg gggacccgaa cgacgccatg gctgtcgtgc cccgcgagat
    12481 tggcggaaac gagagatttg ccgagccgct ccccttcctc tcgcgagcag cccgctgcaa
    12541 tgaagatgtc gcgcacaagc tcgcgcaggt tctccgcagc cactgtgacc atgcgtaacc
    12601 cccaggtctt atctccccgg ctcgcatagc cggctttgaa ggcgaagcta aagcagatca
    12661 ggcccgcttg tcaccaaggc tgaagtgaat ggccgctcca ataggcgcga agcgcacctg
    12721 gcattgatct tgatcacttg cgtccgcttt cgaagccgct agcttgcgag cccaaacggc
    12781 agaaagggct cctcaatgga ttttgctttg cccaaggatc tcgaggcgct gggcgagcgg
    12841 gtgctcacgt tcatcaagga agagatcatt ccgttcgaaa cggacccgcg atggaccgcg
    12901 cacggcccca ccgaagagct gcgccgcgag ctcaatgacc gggcacgcag ggctggtctg
    12961 ctgtctgtgc atgttccaaa ggaatatggc ggcctcggcc tcgatcacgt cggcaagtcc
    13021 gtggtgctgg aggcggccgg ctactccatg ctgggcccca cggcgctcca ttgtcaggcg
    13081 cctgatgaag gcaacatcca tctccttgag caggtggcca atcccgagca gaaggagcgc
    13141 tatctgcgcc cgctggccac ggcggaggtt cgttcctgct tcgcgatgac ggagccccac
    13201 ccgggagcgg gctccgaccc cggcatgctg gcgacacgag ccgagaagga cgggaacggc
    13261 ttcgtcatcc gcggcaagaa gtggctcatc accggggcgg agggggccgc gttcgcgata
    13321 atcatggctc ggacgggcga tggcccgaac gacgcgacca tgttcatcgc cgacctgccg
    13381 aacccggcgt tcaagatcac ccgcacgctg gacacgatgg attcctcgtt catcggcggc
    13441 catgccgtgg tggagattga ggatttgcgg ctggcaggtg atgccgtgct gggcgagatc
    13501 ggtcaggggt tccggtatgc gcaggtccgg ctggcgccgg cccgccttac gcattgcatg
    13561 cgttggcttg gatcggctca gcgggcccac gacatcgcct ccgaatatgc gcggactcgt
    13621 caggccttcg ggcagcggct cggcgcgcac gagggcgtca gcttcatgct cgccgacaac
    13681 gtgatggaca tgcacgtgag ccgactggcc atccggcatt gcgcctggtt gctcgaccag
    13741 gggaaccgcg catcctcgga gagctcgatg gccaaggtta tctgctcgga ggcgatcatg
    13801 cgcgtcgtgg accgttcgct gcagatcctt ggcggcacgg gcctgacgcg cgataccgtc
    13861 gtcgagcgga tattccgcga tgtgcgcgcg ttccgcatct atgatggacc gtcggaggtg
    13921 caccgctggg cgctgggccg gcgcgtcgtc gagcggaagt ggtcgaacaa gggttgagac
    13981 ggcccatgcc tttcaggcgg aagccgctgc cctcgacttg cggacgctgt ccaggatgat
    14041 atccgctgag cggacggagc aggcgcaggg cacgtcataa aggatcgaca gcctcagcag
    14101 cgccttgaca tcgacatcgt gcggcatggg cgtcagcggg tcgatgaaga agaacagcac
    14161 gtctatggcc ttcgcaacga tgagcgcgcc gacctgctga tcgccgccgc gcgggccact
    14221 ctcgagccgc tccagccgca gctctgggta gtgctccagc agcacctttc ctgtcgttcc
    14281 cgttgcgatg aggtcgagct gccggaagat gtcgagattt ctgccgaccc aatcgaccat
    14341 gagcggcttg cgcgcatcat gcgcgaccag agcgtacttt tttgtcattg caaccaagta
    14401 tcccgcgatc ggaggctcga caagctaacg catatcagct cgaggtgtcg cgggggccgc
    14461 cggcagttgt gcgccgcaaa aacgggaacg acgccgtaac gccgtccccg gttccatgtc
    14521 tccccagact tggaccgtca cattgattgc cgtttggttc ggactgtcga acggcacggc
    14581 tatgtgatcg actgcggcag gatgtcgtag gccgaagccg ctgtcaagga tgtcgcacgc
    14641 gcattggaag gaggttcgcc tctggcctaa gcaccccgat ctccaagccg gttgcacatc
    14701 ggcgacgccg agggtatgcg caggagaaga tcatctgttc acatcttccc cgggtcagtg
    14761 cgcccgccct ctcgtccgtc gctagcctaa ggatctcgac ctgtgccgaa acttgcatcg
    14821 cgtgctcagc tgcctgaagc gggggagatc ttcctcgacc acgtagcctg gatggtgccg
    14881 gacattgcga aggcggcgac ggttttcgag caactcgggt ttccgctcac gccctacgcc
    14941 gtgcatggca atcgcgatcc tgtgaccggg gtgctcgatc cggtggggac cgcaaaccgg
    15001 ttggccatcc tcgagcgggg atatctggaa atcctaaccg ccgtggatgg cctcgatacg
    15061 ccctcgacac ggcatatgaa gaaagccatc gaccatcaca cgggggtgca cctcctcgcc
    15121 ttctctgtcc ccgatgcaac cgaggctgcg cgcgagatcg cggcgcgcag tgtcgaactg
    15181 caacctaccg tacacctgag gcgcacagtc gagacagaca gcggggagca ggccgaggcg
    15241 tcattcacgg tggtgcgggc agctttcgag cagtttcccg aggtccgggt tcaggtcctg
    15301 acccaccata ccgccgagca tgtgtggcag cgccggtttc tgtcgcgcga gagcgggctc
    15361 gtggggctgc tggaggccgc gatcgtcacg gccaatccgc acgaggcggc gttgcggttc
    15421 actgccttcc tgggccggcc ggtcgagaag gacggcagct cgctgctaat aagactggat
    15481 cgcggtgctc tgcggatcgt cgatgcacgg ggcgctgccg agcggtacgg ccgaatctcc
    15541 aggccgccga tgccggccgt ctgcgccatc acgctcacct ccagcaatct tgaccagacc
    15601 cgctacttct tcctgagcca ggggctgcgg cctggagcga tcaacccggg tcatctgctg
    15661 atcgacgagt cggaggccct gggcgtgcat ctcgtcgtcg ttccggattg agccaagccc
    15721 tctccggcgt gaacggtcaa ggggcgaggc ccgccagtgg aggtcgtcaa ccctcaccgg
    15781 cccttcggaa aacgcaatgt cccgaagttc ggaagcgaac ttcgggacat tggatggtcg
    15841 tatgtgattg aggccgagag agtcgccgga cgcgcaagtt gcgagcacca gcgacattct
    15901 cgatcgtcgc actaccagtg ggccagcacc gcaagcagca acagtgctac gatgttggtg
    15961 atcttgatga gagggttcac cgcggggccg gccgtatcct tgtaggggtc gccgaccgtg
    16021 tcgccggtga ccgacgcctt gtgcgcctcg gagcctttga tgtgcttcgt gccatccttg
    16081 tcgacgaagc catcctcgaa ggatttcttg gcgttgtccc aggctccgcc gcccgaggtc
    16141 atcgagatgg ccacgaacag gccggtcacg atgacgccga gcagcatggc gccgactgcg
    16201 gagaaggctg cgctcttgcc ggctgcgccg ccaccagcga ccaggtagat caccaggaag
    16261 aggacgatcg gcgacagcac gggcagtagc gacgggatca ccatctcctt gatggcggcc
    16321 cgggtcagca ggtccacggc cgcggcatag tcaggccgct ccgtgccctt catgatgccg
    16381 ggcttctccc ggaattgccg gcgaacctcc agcacgatcg cgccacccgc gcggccgacg
    16441 gccgtcatgg cgatgccacc gaaaaggaac ggaatgagac cgccgatcag caggcccacg
    16501 acgacatacg ggttggagag ctggaagtcc ggctgcacgc cctggaagta ctgataggcg
    16561 gcagagcctg ccttgttcgc ctcggagatg aagtactgga ggtcgtagct gtaggctgcg
    16621 aacaatacca gagcaccgag gccggccgag ccgatagcgt agcctttcgt gaccgccttg
    16681 gtcgtgttcc cgaccgcgtc gagtgcgtcc gtcgagcggc ggacctcact aggcaggccc
    16741 gacatctcgg cgatgccgcc ggcgttgtcg gtaaccgggc cgaaggcgtc gagtgcgacc
    16801 accatgccgg caaggcccaa catcgtcgtc gtcgcgatgg cggtgccgaa cagaccggca
    16861 aggttatagg tgatgatgat gccggcaacg atcacgaggg tcggcagtgc ggtggcttcg
    16921 agcgagatgg ccaggccctg gatcacgttc gtgccgtggc ccgtgacgga ggcctgcgag
    16981 atcgagcgca ccgggcggta gccgatgccc gtgtagtact ccgtgatcca gacgatcagg
    17041 cccgtgaccg caaggccgac gagaccgcag atgaacaggt ccgtccccga aatgacctgc
    17101 ccggcggcgc tgccgacctc accccacgtg cccagcacgt actgcgtcgc aacgaaaaga
    17161 ccggcgatcg acagcactgc cgaggcgatg aagccgcgat agagcgcgcc catgattgag
    17221 ttgttggcgc cgagcttcac gaagtacgtg ccgacgatcg aggtgatgag gcacgctgcg
    17281 ccgatggcga gcgggtagat catcaggctc gtcaggttgg gctgcccgag gaagaagatg
    17341 gccgccagaa ccattgtggc gaccacggtc actgcgtagg tctcgaacaa gtcggccgcc
    17401 ataccggcgc agtcaccgac gttgtcgccg acgttgtccg caatggtggc cggattgcgc
    17461 ggatcgtcct ctggaatgcc ggcctcgacc ttgccgacaa ggtcgccgcc gacgtcagca
    17521 cccttggtga agatgccgcc gccaagacgg gcgaagatcg agatcagcga ggcgccgaag
    17581 ccgagcgcca ccagcgagtc gacgacgacc cggtcggcgg tgccgaagcc gaggcccacg
    17641 acgaggaagg tgtagtagac ggcgacgccg agcagcgcga ggccggcgac aagcatgccc
    17701 gtgacggcgc ccgccttgaa ggcgatgtcg agcccgctcg ccagcgacac cgtagcggcc
    17761 tgtgccgtgc gcacgttggc gcgcaccgac acgttcatgc cgatgtagcc ggccgctccg
    17821 gacaggatcg caccgatggc gaacccgacg gcggtccaca cgccgagaag aaccaaagcg
    17881 aggatgaaga tcacgacgcc cgcgatggcg atggtggtgt attgccggtt gagataggcg
    17941 ctcgcgcctt cacggatcgc gccggcaatc tcctgcatct tctgattgcc ggggtcggcg
    18001 ctcatcaccg acttgatggt aactcctgcg tagatgatcg agatcagccc agcggcgatg
    18061 atcacccaaa gcgctagatt catgagatgt ttccttcgtg ccgaagtctg ttcgcggcga
    18121 cgctggcgca gcagatccgc cgtcgccgag catggccccc ttggagagag agtggtgcga
    18181 gattcgtgcg gggcggacca tgccaaaact gctctgccgt tgcaacatga acacaaggct
    18241 actttagcta taggcggggc tctgcagtgc ggcctggtgc cgccgcagcg caccgatgtt
    18301 gcagaaaatc accgctggaa gtggtcgtag cccagcctat ggagctcgat cggctttcca
    18361 tcggggccga tgacctgtac gccgctgccc gcctgaagcg atcctattcg ggtaacgggt
    18421 acgccagccg cacttgcgct tgcttcgaaa tctgtggctc gctcggggga caccgcggcc
    18481 aggatctcgt aatcatcgcc gccggtcagc gggagaagtc gtagttctgc ctgcaactct
    18541 atcgcgcggc gggcagcctc ggacacggga accagagcgg cttcgatctc ggctgcaagt
    18601 cctgaggcgc ggcacaggcg ccctaggtcc ttcacgagac cgtcggagat gtccattgcc
    18661 gcgttcgcgt agccgaggag ggcgtcccgc agcgcaaggc ggggttctgg ccgaagatat
    18721 cgcttctcga gataagccct gtctgcagcg gtgagccctg ccggaatgct gagcccaatc
    18781 gcggagtctc cgagggtccc ggatacgtag acgaaatcgc cagcttgagc cgttccgcgc
    18841 cggaccatgc ggccgctcgg aacgctgccg atcgccatta tggtcaccga caggggggca
    18901 ttcgaccgcc ggtctgtatc gccgcccatg agcacgaggc cgaagtgttc ctgtgcggct
    18961 ttcaggccac cgctgaactg ctccagccag cttcgatcgg gcattctggg gaaggcgagc
    19021 gagagcagat acccgaccgg gcgcgccgcc ttggccgcaa ggtccgatac gtttactgcg
    19081 acggccttcc aggcgatgtc ttcgggccga tcggtggcgc ggaaatgcac gccctcagca
    19141 attgcatcgg tcttcagaac gagatcatgc cccggctccg gcgtgagcgc ggcgcaatcc
    19201 tcctcgagcc cgaacgctcc gggaaagccg gcggcgagcg gggcgaaata gctgcggatc
    19261 agggcatctt cgcctgcggt accgtcgtta tcagacatga tttcctggcc tggccgttga
    19321 gcgggaagta gcgcgccccg ccacttggct cactctgtca gcccggggcc cgccaccgac
    19381 ccagctcact cgccgcttgc cgcaggcggg ttggaatccg agcatagggc acgcgagcac
    19441 aaggcacgac tgaagtctag cgggcgccgc cgcgaggcct gtcggggaac tccccggggc
    19501 gcagccgcct tgcaatcttg tccagaacgc cattcaccac cttcggctcg tcctcggaga
    19561 agaaggcgtg ggcgatgtcg atgtattcat tgatgacgac ccgggccggc acgtctggcc
    19621 gctccaaaag ctcatacacg ccggaccgca gaatggccct cagtatggaa tcgacgcggc
    19681 ccaggttcca accggctgcg agctgctggt tgacgagcgg gtcgatgtcg cgctggcgcc
    19741 ggacgacacc gcgcagcagc tcggcaaaga aggtcgggtc ggcgccgacg atggcgtccc
    19801 ccggctcggc ctgccggaag cgcaggtcct ggaactctgc aatgacggcg ttcaggtccg
    19861 tctgggccat gtccatctgg tagaggccct gcacggcggc aacccgggcc gcgctacgcg
    19921 gattccgctt cacggtgtaa tcttcggatg tcatgcgccc tggccttgaa actgccgggc
    19981 gatctcgatg agctgcaggc aggcgcgcgc tgcagcgccg cccttgccat cgcggccgcc
    20041 tttggcgcgc tcgagtgcct gcgcttcggt atcgacggtc aggatgccgt tgccgacggg
    20101 cacgttgtgg cggatggcca cgtccatcag ccagtggttc gcgttgttgc agacgatgtc
    20161 gtagtgcgcc gtctcacccc ggatcacgca gccgagcgca atggcgccgc agtaccagga
    20221 cgtgggggcc gttgcgggga tgaggccggc actgacggcc tgagcgagaa cctgcgggat
    20281 ttcgagcgct cccggcacgg tgatcctgtc ataggctgca ccggctgcat ccagctctgc
    20341 gacggcgccc tccagaagtg catcgcagat gtggtcatag aaccggccct cgatgatgag
    20401 gacgcggcag cggacccgtc ccgccgttgc gccgcccgcg gtgctgaccg tcgtgcgtga
    20461 accccgtccg accatgaatt cctcagccga tcctgcgttg ctcgacgatc ttcagtccgt
    20521 agccctcgag cccgacatag accgaagccg gggagttcgt aagaagcacc atgtcgcgga
    20581 cgccgagatc gcgcaggatt tgcgagccga cgccgatttc aaccagtcgg cgctcctcgc
    20641 cttccgtctt tcgcggcgcg aggcccgaac gctccccgat ccaattggat acggacttcg
    20701 gcctgagatc gcgtatgagc accaggacgc cgcggccttc ctgcgcaatg gcgcgcatgg
    20761 actgcgcgat gagcgagtcg ccggccgcct ccgtactggt tccgatcccc agcatgtcgg
    20821 acagcatgtt gacggcgtgc acgcgcacca gcaccggtcc gggtttcgtc aggtcgccct
    20881 tcaccagagc caagtgctcg accggctcga ccgtggtttc gtagacgtag agctcgaagc
    20941 tgccgccgaa tgcgctctcc acctgccggc gcgcgacagg tttcacgatc cgatcgttgc
    21001 ggaggcggaa tgcgatgagg tcctcgatgg ttccgatctt gaggccatgg cgctgggcga
    21061 acggcacgag atccggcatc cgcgccatgg tgccgtcctc gttcatgatc tcgcagatga
    21121 cggctgccgg gtagagcccg gcaaggcgcg caagatcgac cgaggcttcc gtatgcccgg
    21181 cacggacgag aacgccccct tcgcgcgcga ccagcgggaa cacgtgcccc ggcgagacga
    21241 tatcgtcatg atctttggtc ggatcgatcg ccgtcgcaat cgtccgtgca cggtcgtgcg
    21301 cggaaatgcc ggtggtgatg ccttcgcgcg cctcgatcga cacggtgaag gcggtgccgg
    21361 tccgggaggc attggcccgc accatcgatt ccagccggag ctgctccgcc cgggccttcg
    21421 tcagcgcgag gcagatcagg ccgcggccat gcttggccat gaaattgatc gcatcgggtg
    21481 tcgccatctg cgccgggatg atgagatcgc cttcgttctc gcggttctcg gcatccacca
    21541 gaatgaccat gcgtccgttg cgcaggtcat ccagcaactc cggcgtggtg gagaggacgc
    21601 cctgcccctc cgagtactgg acggtatcga gggactggtt caccggcgaa actccaaaag
    21661 gcgcgccaca tagcgcgcga aaagatcaat ttcgaggttg acgagatggc ccgccgtttt
    21721 ggctccccag gtcgtcaccg tcaaggtatg cgggatgagg ttcactccga agcggttgcc
    21781 tgcgacctcg ttgaccgtca gcgaggttcc atcgagagcc accgagcctt tcggtgcgat
    21841 atagagcgca agatgttccg gggcctcgaa cgtgaaccgc cggctttcgc cgtcgggctg
    21901 gatgtcgacg atccgggcga cgccatcgac gtgacccgtg acgatgtggc cgccgagctc
    21961 gtcgcctgcc gcgagcgagc gctccaagtt gatccgccgg ccgggctgcc agctctcgat
    22021 cgtcgtcttg gagcgggtct cgttcgaggt atcgacgctg aacacgctgc cctggccggc
    22081 cggctcgaca gcggtcacgg tcaggcaaca gccgtcacag gcgatagagg cgccgagctt
    22141 gatcgaatcc gccgcatagg acgagcggat cgagaaccgg ccgccgtcgc gcgcagcgat
    22201 ctcgccgata tccgtgacaa tgccggtaaa catggtgctc acgccttctt gagttttcgt
    22261 tcgtttctcg tttcggggct gtgtggggct cactcacgcg ggccgattgg ggcggcggaa
    22321 tatcatcata tcatcgggac cgaattcgcg ctgcgccgtc agcatcaaat cgacgcccgg
    22381 gaggtaggca gcgggcggcc gtctgacaat gcccgcccgg ccgcgcgcct ggaataccac
    22441 ggcttcgtcg acaaagcctg cccgggcgaa cgagcgccac accgccggac ccccttcaac
    22501 gaggagccgc gtgatgcttc gagcggccaa accctcggtg accgccggaa gccagagcat
    22561 gccctcgacc acgcgaacgg gtatcacctc acagccatgc ttctccagtg ccgcccgctt
    22621 cctcgcatct gcgtccgctc cgcagaacac catcaccggt acatcgcggg ccgagcggat
    22681 aagcttgctt tcgatgggaa tgtccaggtc gcgcgccagc acgacacgaa caggcgagcg
    22741 gtcagccagg ccgggcagcc ggcaggtgag ctcggggtcg tcgtctcgca cggtccggct
    22801 gcccacgagg atcgcatcgc tcatcgcccg cagcagatgc ccctgggcac gtgcctccgg
    22861 cccggtgacc cagaccggtt tgccttccgt gccgcggggc acggagccgt cggcggaaag
    22921 ggccagcttg agctggatga acggtcgccg ttcgctgacg cgcagaatgt gcccgcgcgc
    22981 gacccaatcc gcctcctcgc tccggacgcc gcgggtaacg gaaatgccag cggcccgcaa
    23041 acggtcgagc ccgcgcccgg caactctcgc gtcgggatcc tcgatgccga cgacgacacg
    23101 ggccaatcct gcctcgacca gcgcgtcggc gcagggcggc gtgccgccgt gatgtgagca
    23161 tggctcgagg gagacgtaca tcgttgcccc ccgcgcgcgc gctccggccc ggcgcagtgc
    23221 ctccgtctcc gcatggggcc gcccgccggg ctgggtccag ccgcgcgcga tcacctcgcc
    23281 cgaggcttcg tcgacgatca ccgcacctac cgccgggttt ggagccgtgt ttccgagccc
    23341 gcgccgtgcg agcctgaggg cgatgtccat gaagcggcca tcccgctcgg actgggtgtc
    23401 actcgcgatc gttctggcca tgggactcct ggccggcgcc atttcctttc gcgatctcgg
    23461 tcagaacctc ctcgaagtcc gacgcctcgc gaaaatcgcg gtacacggaa gcaaatcgaa
    23521 cgtaagccac cggatcgagg ccgcgcaggg cttcgcagac cgtctcgcca attgcggagg
    23581 atgtcacctc cgattctcca gtgctttcga gctgacggac gatacctgac accatgcgct
    23641 cgatccgctc ggcttcgacc ggtcgcttgc gcagggcgat ctcgatggac cgggccagct
    23701 tgtcgcgatc gaacggcacc ttgcgacccg atcgcttgat gaccgtgatt tcccggagtt
    23761 ggacgcgctc gaaggtggta aacctgccgc cgcaatccgg acagacccgc cgccggcgga
    23821 tcgccgcgcc ctcttccgtg ggccggctgt ccttcacctg cgtatcgccg ctgccgcaga
    23881 acgggcaacg catgggtgat ctctcctcat cggaacgact gagtatgccg ctgccgggct
    23941 attcagttaa ggtcatcccg gtgcttgtca ccgggatcca gatttccgct tgctccggcg
    24001 cttgcggtga gatggatccc gggcacaagg cccgggatga cgattcaatt atagatcgga
    24061 aacttccggc agagctcgat cgcttcttcc cgcacgcgcg cctcgacggc tgcatttccc
    24121 tcctcgccgt tcttcgccaa gccgtccagc acgtccgcga tcatgcggcc gatggccttg
    24181 aactcatcga tcccgaatcc gcgggtcgtg cccgccggag agccgagacg gatgcccgat
    24241 gtgacggtcg gcttctccgg atcgaacggg acgccgttct tgttgcacgt gatatgagcg
    24301 cggccgaggg ccctttccga gatattgccg gtcagtttct tcggtctgag atcgaccagc
    24361 atcaggtgcg tatccgtacc gccggatacc agatcatagc cggaagcctt gagcacgtcg
    24421 ccgagcgccg cggcgttctc gacaacggcc ttggcgtagg ccttgaattc cggcctcagc
    24481 gcctcgccga aggcgactgc ctttgcggca atgatgtgca tcagcggccc gccctgcagg
    24541 cccgggaaga tggccgagtt gaccttcttg gcaatctccg ggtcgttggt cagaatcatg
    24601 ccaccgcgcg ggccgcgcag cgtcttgtgc gtggtggtgg tcaccacgtg ggcgtgcggg
    24661 acgggattgg gatgggcgcc accggcgacg agtcctgcaa aatgggccat gtcgaccatc
    24721 aggtacgcgc cgagctcgtc cgcgatctcc cggaacgcgg caaagtcgat ggtgcgcggg
    24781 taggccgatc cacctgcgat gatgatgcgt ggccggtgct ccttggcgag cgagcggacc
    24841 tcgtcgtggt cgatacgatg gtcctggcgg cggacgccgt aatgcaccgg cttgaaccat
    24901 ttgccggaca tgttgacggg tgagccgtgc gtgaggtgtc cgccggccgc gagcgacagc
    24961 ccgagaatgg tgtcgcccgg cttcgccaga gccatgaaca cgccttggtt ggcctggctc
    25021 ccggagtgcg gttgcacgtt ggcgaatgcg caaccgaaaa gccgcttggc ccggtcgatg
    25081 gcgagctgtt cggcgacgtc gacaaactgg catccgccat agtagcgacg ccctggatag
    25141 ccttccgcgt acttgttcgt gagcaccgat ccggcagcct cgagcaccgc ccgcgagacg
    25201 atgttctccg aggcgatcag ctcgatctcg ttctgctggc gttccagctc gttcgcgacg
    25261 gcgccgaaga gctcgggatc acgctcggca agggcctcgg tgaaaaagcc tggcgaggca
    25321 tttggtcggc cgggactggc ggtgctcata gtcatgctct ctcgcgggaa gacggcgtag
    25381 cctttgccga gcggttctgg ctgggccccg agcgacataa cacgagcgtc agccggccaa
    25441 caaccgcgcg ggctggcttg ggacgtgatg gcgcggggca tctctctctg agataatgcc
    25501 tgacggcgga cgactactcg gccgcgaccc ggacggggcc caactccagc ccgaattcac
    25561 cccaaaggac cgtaagcgcc gatacgaggt gatcgatgtc gcgatcggaa tgcagtggcg
    25621 tcggcgtgaa tcgcagacgc tccgttccgc gtggcaccgt aggatagttg atcggctgga
    25681 cgtagatgcc gaagcgggcg agcagggcgt ccgaaatttt cttgcacagg accgggtcgc
    25741 ccaccatcac gggcacaatg tgactcgggt tatccagcac gggaagaccg gcggccgtga
    25801 gccgacgctt cagcgtgcgg gcccgttcct ggtggcgctc ccgctccacg gcgcttgctt
    25861 tcaggtgccg gacgctggcg agcgccccgg cgacgatgac aggagccagc gaggtggtga
    25921 atatgaagcc tggcgcgcag cttcggatgg cgtcgatgca ggggcggctg gccgcgatgt
    25981 agccgcccat cacaccgaag cccttggcga gcgtcgcgtt tatgatgtcg acccggtcga
    26041 ggacgccatc gcgctcggcg atgcctccgc cgcgtggtcc gtacatgccg accgcgtgca
    26101 cttcatcgag ataggtgagg gcgccatacc gatcggccag atcgcagatc gccgcaatcg
    26161 gggcgatggt cccgtccatg gagtagacgg actcgaaggc aatgatcttc ggcgtctcgc
    26221 gatcgaactc cttgaggtgg gcctccaggt cggcgagatc gttgtgccgc cagatccgct
    26281 tcggcccgcc gccacgccgg atgccttcga tcatcgaggc atggttcttc tcgtcggaga
    26341 agatgacggc gccgggaagc agcttgacca gcgtcgagag ggtcgcatcg ttggcgatgt
    26401 aggccgacgt gaagagcagg gcggcttcct tgcgatgaag ctctgcgagc tctgcctcca
    26461 gctccacatg gtagtgggtc gtcccggaga tattgcgcgt gccgccggac ccggcccctg
    26521 tcgtgtccag cgcctcgtgc atggctgaca gcaccaaggg atgctgcccc atccccaggt
    26581 agtcgttgga gcaccagacc gtgatcgcct gcttcgagcc ggagccgtag tagctggccg
    26641 ccgggaacgc cccgcaattg cgcctcaggt cagcgaatac gcgataccgc ccctcggcct
    26701 ggatctcctc cagaacattc tggaatcttg ctgtgtaatc cactgatccc gtccttgcac
    26761 gagcctctat gagctttgac ccggcggaga ccgtgagatc aacttccccg atgtcgcggc
    26821 tcaggtccag gcattcgtgt gggaatcaaa gtcgcgcccg accaccttcc tcaataggaa
    26881 gcattcgggc caaaaatgcg ccttgagctc aatcaaggac gtcagtaggc cgcagccacc
    26941 gccccgacgc catccttctg atagaggtcc cgccggaact ggacgacacc gtcctcggta
    27001 gcccaggccg tgatgtagac catgtagatg ggaatgggct tcttgaggct cacgtctttc
    27061 cgctcaccgg actccttcag cgccatcacg tgcgcgaggg tccatccatt gtcatgcaac
    27121 agccatgcag cgagctgctc gatgccctgg acgcggacgc agccggaggt cgccgcccgg
    27181 aagttgcgcc cgaagatgct ctccgaaggc gtatcgtgga ggtagacgga gtagccattg
    27241 tggaagttga tcttcaggaa cccgagcggg ttgtccttcc ccggctgctg tcgataggtc
    27301 aggtggtgta ccatctctga agaccagtcg atcttcgagc tgtcgagctt gcgcccgtcc
    27361 gggccgtagg catcgatccc gtagacttgc aggacatcct tgttgctccg tgccagctcg
    27421 cggcccttgg ggatcaggtc tttcgcgatg acggtcggcg gcaaggtcca gattggattg
    27481 aagttcagct cgcgaatggt cgagcggagc accggggtag gccggtcgat cttgcccacc
    27541 acgcccgcgt gccgggacac gacgcggtcg ttctcgatcg cttcgatctg cgcagccggg
    27601 atgttgacca gtacataacg tttgccggcc gtgcgcgcca gctcgctgac ccggtccagg
    27661 ttcgtccgga gctgacggag cctggccgcc gccggcacat tcaaggcgcc gatggtgcgg
    27721 tggtcgacga tccccgtcgg cgcgaggccg ttggatgctt ggaaccgctt caccgcgcgc
    27781 tcgagatagt agtcgaagta gtcggggtag ttggagcctt cccgcaggtc tcccgacatg
    27841 agcaggcgct cacgaaggat ccgcacggcc cgatcggtca ttccggcctg cagcctgacg
    27901 ttggggaccg gcttccagcc gccttgctcg acgatagcgg catagcgctt gatggcggcc
    27961 ttcatcggcc cgatgttggc cggtgagacg gtggggtatc cgcgtggcgg gtttgcctcc
    28021 cattcgcgga tgaaggcccg gtcatagggc tgcgtgtagc tggatacggt gtctgcaaag
    28081 gggttccagc tcgcgctgtc gtaagccgcg gccggcgtgc cggtcacgat cgatgccgtg
    28141 atccccgctg cagcgaagcc aatcaggttg gccaacctcc ggccgctgaa tgcccgtact
    28201 gccaccacgt cgcgcactcc tcgcctctgc tcaaccttcg cccagaatct cacgccagtt
    28261 agtgacaaca gcgttaacga attctgagcg tacgtcgcag ctcagaccac gtatgaattc
    28321 acgagcacga cggagcgttc ccgacggttg atgacgggac cgccgcgcac cgtttggttc
    28381 aggctgtcct gcaggggctc aacattccct caacgcaata aggcggggaa gacacaattt
    28441 tgttcggttt gcctcaattc gaggccagcg atgccgcttc gcaacagaaa accccggcgc
    28501 gagccggggc attgaacggc gagcttgcaa attcggcggt cgtcagagcc gatagcggat
    28561 ctggtcggac cagaaccgct cgagacgcat gagcacgcgg ttcagatcct ggagatcatc
    28621 gaagccgacc tcgcccacgc ttcccaagga gcggagctga cggcggtaga ggcggcgcac
    28681 ggtttcgcag atcgcctcgc ccttgctcgt cagtcggact cgtaccgagc ggcggtcgtg
    28741 atcggaccgc ttgtagtgaa tgtagcccag ctcgaccagc ttcttcagat tataggagac
    28801 gttcgagccg agatagtagc cgcgcgtctt gagctcacct gccgtgagct cctcatcacc
    28861 gaggttgtgc agcagcaaag cttgaacggg gttgagatca cacccgccgt tgcggtcgaa
    28921 ctcgtccttg atgacatcga gcagcaatcg atgcaggcgc tcgatgagcc gtagcgcctc
    28981 caggtactcc ccctcgaggt cattcttggc gatcggcacc ggctgcgcct cgacgtgaag
    29041 gaatctcatg tctgcccccg caaatttccg tccaacggcg gccaggtcgg ccgctatcga
    29101 gccgaagcta aagggggcac gctgaatcac cgcctaataa atctggtgag caaatcctaa
    29161 agggttgctc cgcctcgcct cactagaagc gggccgcctg catctcctga cgggtcggca
    29221 ggatgagctc gcgtccgggt tgaaaggcga agatgcggtc gacggttgcc tcagagactt
    29281 cggacagatg ctcaggaatc cagcgaggtc ggtcctggtt ggcgagcaga acccgcactc
    29341 cttcacgaaa atcgtgagat ccaacgaggc gacaggccag ccggcagtcg atcatcagcg
    29401 tctgccgaag atcgcggaag gctgcttcgc ggatgtgccg cagcgtgatc ttgagcgcgg
    29461 tcggagagcg ccggctgaga tcctggagca catcttcgca ccagccgccc gcagcgccgc
    29521 cacgctgtgc ctcggccctc agccgactgc agatttcctc caccgtttca gctgcgaagc
    29581 aacgctgcag gacctcgcga taggactcga tctcgcccgg ccccggctcc tcgtggcggt
    29641 cgtcgagaag cggatcgaca ggatcggcgc tccgcaagcc cgcttcgatc tcgctgaagc
    29701 ggctggccgg aatgcaatgc gtgaccagcc cgagccgata ggcatccgca cggccgatgc
    29761 ggcgccccgt caggccgaga tagacgccga tctcgtccgg cattcgggcg caaacccatg
    29821 agaggccagc gtcggcaaaa aagccaaaac cggtctgtgg caaggcgaag gagaaggtct
    29881 caccagcgac ccggtgcgtt ccgtaggcgg tgagacccgc tgccgatccc atgatgaggc
    29941 cgtcgatgag cgagatggtc ggcttggaaa agcattcgag aatccagctc agacggtaag
    30001 tgtcggcgag gagctggcgt gcgcgttcgg gctgctcttt cgcggcgctg ccaagctccc
    30061 gcaggtcgct gccgaccgaa aaggctccct gtgtcccaga gcgcatgatc agcgcgtaaa
    30121 cgcccgggtc gcgggcaaac ctcgggatcg actcggcgat ccgggaccgt atggcccctg
    30181 aaagggcgtt gccggactgc ggacggtcga gcgtaacgat ggcggcggca ccttccaccg
    30241 cgatcgtaag ctcttcgcaa gattccttgg ctgatccttc aggcatgtgg ctttcgattc
    30301 gggctcaccg ggtcaggaca tgcgctcagg ccgcgcctgc catactttcg cggccgactg
    30361 tgacctgttc acagcgggtc tggccgccgt gcttgacgcg tgcgccgcaa cggtgaacgc
    30421 tgaggcacgc ttgatgtcaa gggagcgggg gactgctatg ccgctgaagg ttctggcgac
    30481 tggcgcgagg caggccggct tcgcgctgtt tgcggcggca ttgctctgca cgcaccctgc
    30541 atggtccaag ggagctgctt ccagcgggaa gccggccgag gcgcaggcgc cctctgtgcc
    30601 gaagaacgct gccggagtcg ggaccgctag cgaagggagc aagccggcag aagcggatgc
    30661 cagcgaggcc aatccggccc ccgatgtctg gtcggatcag gagatcgccg aagccaaggc
    30721 ctactgcgac cagattctgc atggcgtgga ggccgtaatc gagccggcag atcccatcaa
    30781 ggagggcgag tgtggcgcac cggctccggt ccggctgctc agtatcggcc gcaaccccga
    30841 agtgacgctg tcgccgcccg tcaccgtcac ttgcgatctg gtcgtgaggc ttgccgagtg
    30901 gctgaaggac gacctgcagc cgctcgcaag gaagcacctc ggcggggaga tcgtgcgcgt
    30961 aaacacaatg agctcgtact cctgccggaa tgcctatggc cgcaagaaga cgaagctgag
    31021 cgagcacggg cgggccaatg cggtggacct cggcagcttc gtcacgggag atggtgtgga
    31081 ggccgacctg ctgaagggct ggggcctgac ggcgcgcgac gtgcagaaaa tcgtcgccag
    31141 gagcaaggct gcgaagacgg cagccctgat ggccaacggg cagtccggtg acccgaaacc
    31201 agcgcccgag cgtaatggaa aaggtcaacc agctctcatg gtggcttcca tggcttcgat
    31261 gggatccatg gttcctcttc cgatcaggaa gccgccggcg gatgtggcgc agtggcaggt
    31321 gcagctgaag ttacggagcg gcaagggcag catccgtccc tcgccagagc tgccgacccg
    31381 gcggccgcag gccgttgcga tgaatgaggc gagccacctc ggcggacctg aggcggagga
    31441 agccgacgtc gcaccggagc ccttcgctcc cgatgatcag gttgcgctct tcctgcgcgc
    31501 ggcccacacc gcggcttgcc gccgcttcgg caccgtgctg ggcccggagg ccaacgaggc
    31561 gcaccggaac catttccacc tggatatggc aaagcgcaag cacagcaact actgcgaatg
    31621 attgttccac cggctgcacg cgcacttgcc ttcggcgcct gagccgacga tggtgatggc
    31681 agaccgatca gcttttgcca gggtccgcca tgacgccgcg aaccggccaa tcaccatccg
    31741 agacactgtc cacgcttgcg ggcggtggca cgtcctttcg gcccggcatg ttcccggccg
    31801 tacgcatgcg cagaaaccgc cgggcggatt gggcgcgtcg cctcgttgcc gagaatgccc
    31861 tgaccgcttc ggacctcatc tggccgatgt tcgtggtgga gggcaccaac actcgggtgc
    31921 ccgtcgagtc catgccgggc gtcgagcggc tctcggtgga cctcgtggtg gccgctgccg
    31981 aggaagcgct ctcgctcggg ataccggcga tcgcgctctt tccctatacc gatccggcac
    32041 tgcgcaccga ggatgcgcgc gaggccttca atccggagaa cctcgtgtgc cgggccacgc
    32101 gcgccgtgcg cgaggccggc ctgaacatcg gcgtggttct ggacgtggcc ctcgatcctt
    32161 acacgagcca cggccacgac ggtctgatgc agggaaacac gatcctgaac gacgaaacgc
    32221 tggaggcact cgtccgccag tccctggtgc aagtcgaggc tggctgcgac atcctcgccc
    32281 cgtcggacat gatggacggc cgtatcggcg cgatccgcca ggcgctcgaa agcgctgggc
    32341 atcagtccac gcagatcatg gcctatgcgg ccaagtacgc ctcggcgttt tacggcccgt
    32401 tccgggatgc cgtcggctcg acaggggccc tcaagggcga caagcggacc tatcagatgg
    32461 acccggcgaa cacggacgag gcgctgcgcg aggtggcgct cgacctcgcc gaaggcgccg
    32521 acatggtgat ggtgaagccg gggatgcctt accttgacgt gctccgcagg gttcgggaca
    32581 cgttcggtgt gccgacgttc gcctaccagg tctcgggaga gtatgcgatg ctgatggccg
    32641 catcggagcg gggttggctg gatggcgata aggtcatcat ggagagcctg ataggcttca
    32701 aacgggctgg cgcggcgggg atcctgacct atttcgcgcc gcgggcagcc aggctgctgg
    32761 cgtcgacacg ctgactttcg cccccgcgtc ggctgcggag gatggccgtc ggctagggag
    32821 gcggtgcgtc gctctcctcg agagatttcg ggtgggctta caggaggttc ggtcggtcga
    32881 gcctgcgtct tttttggttt gatgttgctc gggccgattg cgtggggcag gattcgggca
    32941 ctgctggcac gcttgcgcgg cccggtggtt tccttggagc tcgcggcgcg ttttcccggt
    33001 gatttagcgt ctcaggtgtc acagagcttg attgcgcgca ggtcgctcac ttacttttct
    33061 tgaaggcgag ggccgggagg ccgcatgcgc tcgcccttgt cgccggaaca ccacccgaat
    33121 gacagagcag cagaagaatt tcccagagtt ctacgtgacg gctccgcagc catgcccgta
    33181 tctgccgggc aagctcgaac ggaagctttt cacgcatctg acgcacgaca agccggcggc
    33241 actcatagac aacctgctga agggcggttt ccggcgcagc cagaacatcg cctacatgcc
    33301 ctactgcgag ggctgccagt catgcgtgtc ggtgcgcgtg atcgtggacg agttcaagct
    33361 caatcgcagc ttccggcggg tgctggatgc caacagggat ctggccgtac ggcgtgttcc
    33421 gccgcggccc acggccgagc agtacgcgct cttcagggac tatatagatg cccggcatgc
    33481 ggacggaggc atggccgaca tgaccgttct cgactacgcc atgatggtcg aggacagcgt
    33541 cgtggatacc ttcctcaccg agtaccgtgt ccgcaccgat tccgggctcg attcagccac
    33601 ggcggggacg ctgaccgcgg tagcgctttg cgatcgcctc tccgatggcg tttccatggt
    33661 ctatagcttt tacgatacgg aaatgccgcg tcgcagcctc ggcacgcaca tgatcctcga
    33721 gcacatcgag tacacacgta cgctcggtct gccctatctc tacctgggct attggatcga
    33781 gggctcgagg aagatgagct acaagaccag atttcagccc caggagcggc tgaccagcag
    33841 cggttgggtc agagtttgaa gcactccagc acggggcctt ctccggggcc atttcgatag
    33901 attgccgccc tacttgttcg caaagcgggc tcgtcggcgc agtcatttcc gcggatactg
    33961 gctgcaaacg taccgcgcca cgcattgctg caacgtttcc cccgtcaatg cccgttggac
    34021 gtcgcagaag cgcacacaat cctcataggt gcgacgaggc cgcgggtagc gattgcagtt
    34081 cctttcgaga atgcatcgct tgagagtcat ggattccggc gcattgtccc ggcaatcctg
    34141 cacgcattcg taataggtcc aggttt
//
LOCUS       DASVDU010000308        12196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_917689, whole genome shotgun sequence.
ACCESSION   DASVDU010000308 DASVDU010000000
VERSION     DASVDU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917689"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1432)
                     /locus_tag="VF226_20715"
     CDS             complement(71..1432)
                     /locus_tag="VF226_20715"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX7076468.1"
                     /translation="MTLHNADREQATRLLCEFVAASNPGDLPQAVRREARRAFLNILG
                     CAIGGSRHEIVSLTKDALRDYWGPPQATLLAQGEKTDILHAAYINCLSSSVDSFDDSH
                     AEAIIHPSGPVASVLLALSERRKTTGAEFLHAFALGIEVICRVTKAVCVAPARGGLAW
                     VQTPIVGGLGAAAAAGRLLGLDSRRLGWALGIAGSQASGYRGNHGTMNMPLMPAQAAR
                     SGLFGALLAERGITSSDATLEGRYGFCEVFSEQPNVAAITDRLGESFDLLGTTYKPYP
                     CGIVVNPTLEACLRLKERCSIVPDAVERIEVHANPTAAAIANRRHPADPLAAQVSLQH
                     WAAVALAYGMATKGELSPERIADPGVVRLRDRTEVAEAPPLAEDAAEVVVLLSSGERL
                     NESVDHCIGSANRPMTEAELEGKFRGLSKGLKSDEETDRLIERCWNIDDLDDMEAITR
                     LAS"
     gene            complement(1488..2303)
                     /locus_tag="VF226_20720"
     CDS             complement(1488..2303)
                     /locus_tag="VF226_20720"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF009093.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX7076469.1"
                     /translation="MTYRSIEIEAGIAGKCALVTGSTQGMGWAIARALASQGCHVMLN
                     GLANPEDVQDKIRILRDDYGAKVSFSGADLAEYEQVKAMVRQAEEELGPIEILVNNAA
                     TRNAAKIEDIPKERWDNALAVNLSAPFHLIQFTLPGMKERQWGRIINIASNLGLTAIP
                     NRSDYVANKHGLVGLTRAVAVEALPYKVTANAICPGSTLTPHAERQINERMAQGKKSR
                     EEAVKDFLETKQPSRRFVDPAQIGDLVLFLCSDAASEITGTPISIDGGWMASA"
     gene            complement(2447..4039)
                     /locus_tag="VF226_20725"
     CDS             complement(2447..4039)
                     /locus_tag="VF226_20725"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX7076470.1"
                     /translation="MEYITEGLLSVLTVTSITVLLCGLIIGFITGAMPGFDAVNAAAI
                     VLPFTVHLDAQNALVAMAGIYAGTAYAGAIPAILLNVPGTAGAAATALDGYPMARKGE
                     AARAISIARGASMIGGVMACLLVLPLLGPISQFALEFTSPEMFLVGLIAMTIIGSLIG
                     DSIPKGMLALFLGMLLATMAASGETGRPRFNFGLLELYESFPFVPVTLGVFALAQMFI
                     YVAEDLKTFRLGTSDQEAGAEQNQKSLLQSWREASAGMTEPFKRLAVTTRSSLVGLGI
                     GILPGTGPTIANFVAYGIAKRRAKRPEEYGQGTPDGIIAPEAADNSCVVGTLVPTFTL
                     GIPGSATAAVMMGAAMMQGWAPGPAIMRDHAVEVYTVLWGLLIAALAIGPTTVVLAAP
                     LAKIATTRREVLTPCVILLSLVGAFAVRNSLFDIGLAILFGFIGVLMRRTGFPVVPMV
                     LGLILAPIIESNFIRSMRLSHYSPTIFFDSTLALILWAVLLLVIAASLWGAVKRARLQ
                     KEVDQSLLGRRGEGASVLSDAR"
     gene            complement(4050..4550)
                     /locus_tag="VF226_20730"
     CDS             complement(4050..4550)
                     /locus_tag="VF226_20730"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX7076471.1"
                     /translation="MTQRLLIAGLRLLFPVGAIVFSIAFYFNIRHMGRSETIFPDLLI
                     AGLLLIAALVLAQEFRALWAVRQISEAEAVGRLDVDGLRSSGWWRSLFVALSCLLYLL
                     SINRLGLLPAAFLAYVTCAVPLFPDRSAWQSWLRCLVGAAVAALLLDTVFRVWLGLPL
                     PAPLPS"
     gene            complement(4778..5863)
                     /locus_tag="VF226_20735"
     CDS             complement(4778..5863)
                     /locus_tag="VF226_20735"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX7076472.1"
                     /translation="MELSRTALGSGAMPGKRFGVSKQALKGAAAATAFALALIQGGGF
                     QAAAADDWPNETLRMVIPSGPGGGTDTIARGLQKFLSEELGTSIRAENLPGANHALGA
                     QAVAKSGSDCNTILAIYEPSLVTMAVADAGLDFSYDDFYPLGGIIVEPFAILVPNNSR
                     FKDWQSLWNEIVKHPGEVRVAITSWFDVTTVHLLEMEEQLGVKFNIIRYDGGSAGRAA
                     LLAGETDFGYHILFGSRGVWDDTRTLAHFNATKLHPRGDISTLVDNSPDLKDLVDAKL
                     TDTVLTFSLYVTSGCHSENPERYNKLLNALRNVIDDPAYHETLKELNMESGLLGVLGD
                     DFFNIYKSMRPELEETINRLVVPRLGN"
     gene            6374..7810
                     /gene="leuC"
                     /locus_tag="VF226_20740"
     CDS             6374..7810
                     /gene="leuC"
                     /locus_tag="VF226_20740"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX7076473.1"
                     /translation="MSAARTLAEKIWGAHLVREGDGLEPDLLYVDMHLIYEVTSPQAF
                     SGLRLAGRRVRRPDLMLATVDHNVPTIDRDLPIADEKAARLLRILEENCAEHGIELLR
                     MTSPHQGIVHTIGPEMGVTQPGMTIVCGDSHTSTHGAFGALAIGIGTSEVEHVMATQT
                     LAQRRQKTLAIEVFGALPFGVYAKDMALNIIRRIGVDGGIGHIIEYRGEAVRSLSMEG
                     RMTLCNMSIESGARAGMVAPDETTFRWLKGRRYAPKGEAWEQACEWWESCRSDEDARF
                     DRVVTLDAPDFEPTVTWGTTPAMSAAVTGRIPELSEVRDRRQAERSLTYMGLEPGMRI
                     TDIRVDRAFIGSCTNGRLSDLRAAARVVAGRHVADGVRAMVVPGSTQVRLAAQAEGLD
                     RIFKDAGFEFREAGCSMCNAMNGDLLLPGERCASTSNRNFEGRQGKGGRTHLVSPAMA
                     AAAALYGHFVDVRELDLAPLADAEGEAH"
     gene            7811..8401
                     /gene="leuD"
                     /locus_tag="VF226_20745"
     CDS             7811..8401
                     /gene="leuD"
                     /locus_tag="VF226_20745"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX7076474.1"
                     /translation="MRPLQTVEGRMVPLDKADIDTDQIMPVQFLKHPERRGFAQFLFH
                     NWMEEPDFVLNDSRYEGANILVCGPNFGCGSSREHAPWGLQEYGFDVVVAPSFGDIFR
                     MNCAKIGLLCAATDSETSRELIALASTSPETRAKVDLSGQTITAGGITRPFKIDAYSK
                     KMLIEGLDHIEQTLRHVGEIDAYEASRPPWLPSTHR"
     gene            8510..9505
                     /locus_tag="VF226_20750"
     CDS             8510..9505
                     /locus_tag="VF226_20750"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX7076475.1"
                     /translation="MLTDSLRPAEQRERALRLTEPLRVHWETSGDATRLSDALRDADF
                     LVTENEIPITREILAGAERLRLIQINGHRCLSVDLEAARALGVAVGTLPLVTSQSVAE
                     HTLALIFALARRILPSDRVVRAGSPASAHEPVTVVPGRSFYNWPGLGGQRLLGGTTLS
                     ILGLGDIGVGVAMRAKALGMEVCYWQRYRISAAEEAALGLRYVDFEEAFAVADWLSVH
                     VRFTDETVGLIGEKELALLRPTAFLINTARGPIVDENALVTALRDGRLAGASLDVYWV
                     EPPPANHALHELENVVLTPHCAAGDFETALLEVKAVFDELRDVWQGHPPSYPVTA"
     gene            complement(9574..10527)
                     /locus_tag="VF226_20755"
     CDS             complement(9574..10527)
                     /locus_tag="VF226_20755"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEX7076476.1"
                     /translation="MTLNNEASPSASRSETPAKAVGPGTRLRQAHRAARPLVAPLAHD
                     ALSARMIQAAGFPAINIGGSTLLAARYALPDIGLAALGEMAPAIRDVVDAVPDMPCLV
                     DGDDGYGDARQVARTVAVLERIGVGGLILEDQHRLEKQPGATAARQVVPLETMLEKLA
                     VALATRADPELVIIARTDALGAGGMEEALRRGERYLKQGADGVFVAGAKGREQLERVG
                     QAFRGQWNMAALFEQPDGALLTPGELSGLGFSHIVYPMAMMLRVVGAIEQFLTRLRDF
                     AEGRSATLGAPDAGAPECFSRAVKSAEWNELAERIAKHRLR"
     gene            10802..11650
                     /locus_tag="VF226_20760"
     CDS             10802..11650
                     /locus_tag="VF226_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7076477.1"
                     /translation="MQDLQTHGISLPRLGLGTFRMANDVCQPAVESALALGYRHIDTA
                     EMYGNEEAVGAAVAASGIARGALHVTTKVWHENLAPGAIRRALDASLNRLKLDYVDLY
                     LVHWPSRTMDLPTIFETLCRLQAEGRTRAIGVANFTVNLLRTAIEDIGAPIACNQIEY
                     HVFLDQTPVRSYLASKSIPLVAYTPLARGEVAESPELQAMGRKHGASAAQIALKWLLD
                     QDGVAAIPKAQRTESQRANLEALNLVLDDEDRRVIAALPKNRRLISPPYAPTWDPLPA
                     GVSAIR"
     gene            complement(11764..>12196)
                     /locus_tag="VF226_20765"
     CDS             complement(11764..>12196)
                     /locus_tag="VF226_20765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076478.1"
                     /translation="HKRILYGSGGSLESQGERNGKPLTLWRMGSEAISDGRILDFAPD
                     YRLSPLASQLFGGERVWRYDMPFDEADWRLIALEHHEFASCIATRAAPEVTGEIGRRA
                     LAIVYALFESGKLGRPVGITEVEQGKVDAYQREIDAHLQLA"
BASE COUNT         2259 a         3958 c         3836 g         2143 t
ORIGIN      
        1 gctgcggtca aggcgggcac gcaaatgctc cgcgcggctc aggtcatgcc cgcaaccgct
       61 gcggggctta tcaggaggcc agacgggtta tcgcttccat gtcgtcgagg tcgtcgatgt
      121 tccagcaccg ctcgatcagc ctgtccgtct cttcatcgct cttcaggcct ttcgataagc
      181 ctcggaactt gccctcgagc tctgcctcgg tcatgggacg gttggcgctg ccgatgcagt
      241 ggtcgacgct ctcgttcagc ctttccccgc tgctcaacag aaccacgact tcagcggcgt
      301 cttcggcgag cggtggcgcc tccgccacct cggtgcggtc tcgcaggcgg accacgcccg
      361 gatccgcgat ccgttcaggg gacagctcgc cctttgtcgc catcccgtag gcgagggcca
      421 ccgcagccca atgctggaga ctgacttggg ccgccagggg atcggccggg tgccgccggt
      481 tcgcgatggc agcggcggtt ggattggcgt ggacctcgat ccgctcgacc gcgtccggca
      541 cgatcgagca acgctccttc aagcgcagac aggcctcaag cgtggggttg accacgatcc
      601 cgcaaggata tggcttgtag gtcgtaccga gcaggtcgaa ggattcgccg aggcggtcgg
      661 ttatggcggc gacattgggt tgctccgaaa agacctcgca aaagccgtat ctgccctcca
      721 gcgtggcgtc ggagctcgtg atgccgcgct cggcaaggag cgccccgaag aggccggagc
      781 gggcagcctg ggccggcatc agtggcatgt tcatggttcc gtggttcccg cgatagccgg
      841 aggcctgcga gcctgcaatg cccaaggccc atcccagccg ccggctgtcc agtccgagca
      901 accggcctgc cgccgcggcc gcccccaagc cgccgacgat gggcgtctgc acccaggcga
      961 gaccgccgcg cgccggcgca acgcagaccg ccttggtgac cctgcagatc acctcgatgc
     1021 ctagggcgaa ggcatgaaga aactcggcac ccgtcgtctt ccgccgctcg gacagcgcga
     1081 gcagcacgct ggccacgggg ccgctggggt ggatgatggc ctccgcgtga ctgtcgtcga
     1141 agctgtcgac cgatgagctc aggcagttga tataggcggc gtgcaggatg tccgtcttct
     1201 ccccctgcgc caggagcgtg gcttgtggtg gaccccaata gtcccgcagg gcgtctttcg
     1261 tcagactgac gatctcgtgc cgggacccgc cgatggcaca gcccaggata ttgaggaatg
     1321 cccggcgcgc ttcgcggcgc acggcctggg gaagatcgcc cggattggac gccgcgacga
     1381 actcacaaag gaggcgcgtc gcctgctctc gatctgcgtt atgcaacgtc atttccgaac
     1441 cggctccgca cttcccgcct cccactccgt ctccttcagg accgcgtcta agcgctggcc
     1501 atccaaccgc cgtcgatgct gatcggcgtt ccggtgatct cgctggcggc gtcgctgcac
     1561 aggaacagga cgaggtcgcc gatctgggcc gggtccacga agcggcggga cggctgcttg
     1621 gtctcgagaa agtccttgac cgcttcttcg cgggacttct tgccttgagc catcctctca
     1681 ttgatctggc gctcggcgtg gggcgtcagg gtcgagccgg ggcagatcgc gttggccgtc
     1741 accttgtagg gcaaggcctc gaccgcgact gcccgggtca ggccgaccag gccatgcttg
     1801 ttggccacgt agtcggagcg attgggaatc gccgtgaggc ccaagttgga agcaatattg
     1861 atgatcctcc cccactgccg ctccttcatg cccggcagcg tgaactggat gaggtggaag
     1921 ggggccgaaa gattgaccgc cagagcattg tcccaccgtt ccttcggaat atcctcgatc
     1981 ttggcggcgt tccgggtcgc ggcattgttg accaggatct ctatggggcc gagttcttcc
     2041 tcggcctgcc ggaccatcgc cttgacctgt tcgtactcgg ccagatccgc cccggagaag
     2101 ctgaccttgg cgccataatc gtccctcagg attcggatct tgtcctgaac atcctcagga
     2161 ttcgccaagc cgttcagcat aacgtggcat ccctgggatg ccagggcacg cgcgatggcc
     2221 cagcccattc cctgggtcga gccggttacg agcgcgcact ttcccgcgat ccctgcctct
     2281 atttcgatac tgcgataggt catcagctat ccccgtttca taagtcgatg gttggtgacg
     2341 tgcagccggt aagaggcatc gaaagatgcc tcggcagccg cgtggaacgt tcgtgccgcc
     2401 aagggcgcgg cgccagggat cgtggccggc cggcaaagag ttcaggtcaa cgcgcatccg
     2461 aaaggacgct ggccccctcg cctctccggc ccaataggga ttgatccact tccttctgga
     2521 gtcgcgcccg cttcactgct ccccagaggc tcgcagcgat caccagaagg agcaccgccc
     2581 aaaggatgag cgccagtgtg ctgtcaaaga agatggtcgg tgagtagtgc gacaaccgca
     2641 tcgaccgtat gaagttggac tcgatgatgg gcgcgagaat caatcccagc accatcggca
     2701 cgaccggaaa gcctgtccgc cgcatcagca cgccgatgaa accgaaaagg atggccagcc
     2761 cgatatcgaa aagcgagttg cgcaccgcaa aggcgccgac cagcgacagg aggatgacgc
     2821 agggcgtcag cacctcccgc ctggtggtgg cgatcttcgc aagaggcgcc gccagaacca
     2881 ccgtggtcgg tccgatcgcc agggcggcga tcagcagtcc ccacaggact gtataaacct
     2941 cgaccgcatg gtcccgcatg atggccggac cgggggccca gccctgcatc atggccgcgc
     3001 ccatcatgac cgccgccgtt gcgcttcccg gtatacccag tgtgaaagtc ggcacgagcg
     3061 tcccaacgac acagctgttg tcggctgcct ccggggcgat gattccatct ggggtgccct
     3121 ggccgtattc ctcgggcctc ttggcgcgcc tcttggcgat tccgtacgcg acgaagttgg
     3181 caatcgtcgg accggtgccg ggcagaatgc cgataccgag acccacgaga ctcgagcggg
     3241 tcgtgactgc cagccgcttg aacggctctg tcatgccggc cgaggcttcg cgccaggact
     3301 gcaggagcga cttttgattc tgctcggcgc cggcctcctg gtccgatgtg ccgagccgga
     3361 acgtcttcaa gtcttccgcg acatagatga acatctgagc gagcgcaaag acgcccaacg
     3421 tgacaggcac gaaggggaag gattcgtata gctccagaag tccgaagttg aaccgcggcc
     3481 gtcctgtctc gccgctcgcg gccatggtcg ccagcagcat cccgaggaac agggccagca
     3541 ttcctttcgg aatgctatcg ccgatcaagg acccaataat cgtcatcgcg atcaggccga
     3601 ccaggaacat ctcgggagag gtgaattcca gggcgaattg ggatatcggg ccaagcagcg
     3661 ggaggaccag caagcaggcc atcacgcccc cgatcatcga ggcgccgcgc gcgatggaga
     3721 tggcgcgcgc cgcctcaccc ttcctggcca tcggatagcc gtccagcgcc gtagccgcgg
     3781 cgcctgccgt tcccgggacg ttgagtagta tggccggaat ggcgccggca taggccgtgc
     3841 ccgcatagat gcccgccatg gccaccagag cgttctgcgc atccagatgc accgtaaagg
     3901 gcagcacgat cgccgccgcg ttaacggcgt cgaagcccgg catcgcgccc gtgatgaagc
     3961 cgataatcag cccgcacagg aggacggtaa tgctggtcac ggtgaggacg gaaagcagac
     4021 cctctgtgat atattccatc gcgagtccct cagctgggca ggggagcagg aagcggcaat
     4081 ccgagccaca cccggaacac ggtatcgagc aaaagcgcgg caaccgcggc ccccaccagg
     4141 cagcggagcc agctttgcca agccgatctg tccggaaaca gcgggaccgc gcaggtcacg
     4201 taggccagga aggccgccgg gagcagtccc agccggttga tgctgagcag atagagaaga
     4261 caggaaagcg caacgaaaag cgaccgccac cacccgctgg accgcaggcc gtcaacatcc
     4321 agccggccaa cggcctcggc ctcgcttatc tgtcgaactg cccagagcgc tcggaattcc
     4381 tgcgccagca ccagcgccgc gatgagcaga agcccggcaa tcaggaggtc ggggaagatc
     4441 gtctcgcttc gccccatgtg ccgtatgttg aaataaaagg ctatgctgaa gacgatcgca
     4501 ccaacgggaa acaagagtct caaaccggcg atcaaaagcc gctgcgtcat ggctatgcct
     4561 cccgaaccgg ctcgccgtca ccgatgtcgc cctctcttgg acgagacgag ctcgaaaggc
     4621 tgagcgggtt ccgcaagagg cagcccggca gggggctgga aggcagggag ggcatggccg
     4681 ttcaagctcc ggggtgggat tgctctggtt ccgatcgagc aacggcagac tgacgcaccg
     4741 ccatcgggtc catcagtctg ctctggcgtt cagcgctcta gttgcccagt cgcggcacca
     4801 caagccggtt gatcgtctcc tccagctcag ggcgcatgct cttgtagatg ttaaagaagt
     4861 cgtcgcccag aaccccgagg aggccggatt ccatgttcag ctctttcagt gtctcgtgat
     4921 aagccggatc gtcgatcacg ttgcgaagag cgttcaggag cttgttatag cgctccgggt
     4981 tttccgagtg acagccgctg gtcacataaa gcgagaaggt cagcacggtg tcggtcagct
     5041 tcgcgtccac cagatctttg aggtccggcg aattatccac cagggtcgag atgtcgcccc
     5101 gaggatgcag cttcgtggca ttgaagtgcg ccaaggtccg ggtgtcgtcc cagacgcccc
     5161 ggctgccgaa gagaatgtgg tagccgaagt cggtttcgcc ggccaggaga gccgcccgtc
     5221 cggccgagcc gccgtcatag cggatgatgt tgaattttac gccgagttgc tcctccatct
     5281 cgagcagatg gacggtcgtg acgtcgaacc acgaggtgat cgccacgcgc acctcgccgg
     5341 ggtgttttac aatctcgttc caaagacttt gccagtcctt gaagcgtgag ttgttcggca
     5401 ccaggatggc aaagggctca acgatgattc cgccgagcgg atagaaatcg tcataagaga
     5461 aatccaggcc ggcatctgcc acagccatgg tgacgagaga cggctcgtag attgcgagga
     5521 tcgtattgca gtccgatccc gacttggcga cggcctgcgc ccccagggca tggttcgcgc
     5581 cggggagatt ctcggcccgt atcgaggtcc cgagttcctc cgagaggaac ttctgcagcc
     5641 cccgcgcaat ggtatccgta ccgcctccgg gcccactggg aatgaccatg cgaagcgttt
     5701 cgttcggcca gtcatcggct gccgcggcct ggaagccgcc gccttgtatg agcgccagag
     5761 cgaacgctgt cgcagcggcg gctcctttca gcgcttgctt gcttacgcca aacctcttgc
     5821 ccggcattgc accggagccg agcgccgtcc tcgacagttc cattgttctt tcctcccatg
     5881 gatcgagcgt ttcggttatt tttttatcgg ttcaatagtg cgcggcagtt tatcggttca
     5941 acaccctgtt tgcaagatcg catcaggacc agccaggaag cggcgtcatc tcgaaaaaat
     6001 gcccggctgc attccagctt cacattccgg aatcaagcga cgcatcagac cacccatgac
     6061 ccgattcaaa tccaatatct gccgaaggtc gcctcaaagg acagcccaag ctccatatca
     6121 agatactgat atatatatgg attttcctga catgcgcccg aacccgatcc ggctgatctg
     6181 acccgctgcg gagagcatga cgaatctgtg cgggtgaccg caatttcccc gtaggaatgc
     6241 ggcttgaggg cagagctcca cttggatcct ataccgtaaa cgcacatgcc gttgactcac
     6301 ccgaaacctt ctgcgagcat tttgtttgaa ccgatcaaag aacgaaaatt gaccgcgagc
     6361 gggaggaacg gagatttcgg cagccaggac attggccgag aagatctggg gtgcccacct
     6421 cgtccgtgaa ggagacgggc tggagccgga cctgctgtat gtcgatatgc atctcatcta
     6481 tgaagtgacc agtccccagg ccttctccgg cctgcgtctg gccgggaggc gggtgcggcg
     6541 gcccgatctg atgctggcga cggtcgatca caacgttccg acgatcgacc gggacctgcc
     6601 gatcgcggat gagaaggcgg cccgcctgct tcgaatcctc gaagagaact gcgcggagca
     6661 cggcatcgaa ctcctgcgga tgacctcgcc gcaccaggga atcgtccata ccatcgggcc
     6721 cgagatgggg gtgacccagc cgggcatgac cattgtctgc ggcgattccc acacctcgac
     6781 ccatggggcc tttggagccc tggccatcgg catcggcacg agcgaggtcg agcatgtgat
     6841 ggccacccag accctcgccc aacgacgcca gaagaccctg gcgatcgagg tcttcggcgc
     6901 gctgccgttc ggcgtttatg ccaaggacat ggcgctgaac atcatcaggc ggatcggtgt
     6961 ggatggcggg atcggccata tcatcgagta ccgcggcgag gcggtgcgca gcctttcgat
     7021 ggaaggccgg atgactctct gcaacatgtc catcgagagc ggcgcccgcg ccgggatggt
     7081 ggcgcctgac gagacgacct tccgctggct caagggccgg cgctacgcac cgaagggcga
     7141 ggcctgggag caggcgtgcg agtggtggga aagctgccgg tccgacgagg atgccaggtt
     7201 cgaccgtgtc gtgacattgg acgcgccgga cttcgagccg acggtgacct ggggaaccac
     7261 tccggcgatg agcgcggcgg tgaccggccg gatcccggaa ctcagcgaag tccgtgacag
     7321 gcgccaggcc gagcggtccc tcacctatat gggcctcgag cccggcatga ggatcaccga
     7381 tatccgggtg gatcgggcct tcatcggctc ctgcaccaac ggccggctgt ccgatctcag
     7441 agccgcggct cgggtggtgg cgggccgtca cgtggcggac ggtgtccgcg ccatggtggt
     7501 cccgggctcc acgcaggtga ggctggccgc gcaggccgag gggctcgacc gcatcttcaa
     7561 ggatgccggc ttcgagttcc gcgaggcggg atgctcgatg tgcaatgcca tgaacggtga
     7621 tcttctgctt cctggcgagc gctgcgcgtc gacatcgaac cgcaatttcg agggacggca
     7681 aggaaagggc gggcgcaccc atctcgtgtc gccggcgatg gccgcggcag ccgccctcta
     7741 cgggcacttc gtggatgtca gggaactcga tctggccccc ctggccgacg ccgaagggga
     7801 agcgcactga atgcgaccgc tgcagaccgt cgaaggccgc atggtgccgc tcgacaaggc
     7861 cgatatcgac accgaccaga tcatgccggt ccagttcctc aaacatccgg agcgcagggg
     7921 ctttgcgcag ttcctttttc acaactggat ggaggagcct gacttcgtcc tgaacgacag
     7981 ccggtacgag ggcgcgaaca ttctggtttg cggtcccaat ttcggctgcg gctcatcccg
     8041 tgagcatgcc ccctggggcc tccaggaata tggcttcgac gtcgtcgttg cgccgagctt
     8101 cggcgacatc ttccgcatga actgcgcgaa gatcgggctg ctttgcgcgg cgaccgacag
     8161 cgagacgagc agagagctga tagcgctggc cagtacctca cccgaaacgc gcgccaaggt
     8221 cgacctgtcg ggtcagacca tcaccgccgg cgggatcacc cgccccttca agatcgatgc
     8281 ctatagcaag aagatgctga tcgagggcct ggatcatatc gagcagacgc tgcggcatgt
     8341 cggcgagatc gacgcctacg aagcctcccg ccctccctgg ctgccctcga cgcaccggta
     8401 acgtgctctt tctcgcagcg aggaccaaaa cctgtccact caaccgcaat ccggaaagcc
     8461 ggcgtcggcc gcccccaagg tggtgatcac cagccagggg gcgctccgca tgctgacgga
     8521 cagcctgcgc cctgcggagc agcgcgagcg cgcgctgcgg ctcaccgagc cgctccgggt
     8581 ccactgggaa acaagcggcg atgcaacgcg cctttcggat gccttgcgcg atgccgactt
     8641 cctcgttacc gagaacgaga tcccgatcac gcgcgagatc ctggccggcg cggaacggct
     8701 gcgcttgatc cagatcaacg gccatcgctg tctctcggtc gatctggagg ccgcgcgggc
     8761 tctgggggtt gcggtcggga cgctgccgct cgtcactagt cagagcgtag cggagcacac
     8821 ccttgctctc atattcgcgc tggcgcgccg gatactgccg agcgaccgcg tcgtgcgggc
     8881 gggcagcccc gcatcggcac acgagccggt gaccgtcgtt cccggccgct ccttctacaa
     8941 ctggcccggc cttggcgggc agcgcctgct cggcggcacg accctgagca tcctgggact
     9001 cggggatatc ggcgtcggcg tcgccatgcg ggcgaaggca ctcggtatgg aggtctgcta
     9061 ctggcagcga taccggattt ccgcggcgga ggaggccgcc ttgggccttc gctacgtcga
     9121 tttcgaagag gccttcgcgg tcgccgattg gctgagcgtt cacgtccggt ttaccgacga
     9181 aacggtcgga ctgatcggcg agaaggaact ggccctgctg cgaccgacgg ccttcctgat
     9241 caacacggcc cgcgggccga tcgtcgacga aaacgcgctc gtcaccgcac tgcgcgacgg
     9301 ccgccttgcc ggtgcgagtc tcgacgtcta ctgggtcgag ccccctccgg ccaatcatgc
     9361 tctccacgaa ctggagaacg tcgtgctgac cccgcactgc gcggcgggcg atttcgagac
     9421 cgccctgctc gaggtgaagg ccgtgttcga cgagctccgg gacgtgtggc aggggcatcc
     9481 gccgagctac ccggtaacag cctgagcgag acccaccttc ccggaccttt tgccctgacg
     9541 atcaggcgga atcgtgacgc tacatccagc cgctcagcgg agccgatgtt tcgctattcg
     9601 ctccgccagc tcgttccatt cagcggattt cacggcacgg gagaaacact ccggcgcgcc
     9661 cgcatccgga gcgccgagcg tcgccgacct gccctccgca aagtcccgca accgggtcag
     9721 gaactgttcg atggctccga ccacgcgcag catcatcgcc atcggataga cgatatgcga
     9781 aaagccgaga ccgctcagct cgcccggcgt cagcagggcg ccgtcgggtt gctcgaagag
     9841 cgccgccatg ttccactggc cccggaaggc ctgccctacc cgttcgagct gttccctccc
     9901 cttcgctcca gcaacgaaga cgccatcggc cccctgtttc aggtagcgct cgccacgcct
     9961 cagcgcttcc tccatgccgc ctgctccgag cgcatcggtc cgggcgatga tcacgagctc
    10021 cggatccgct cttgtcgcca gggcgaccgc aagcttttcc aacatggtct cgaggggcac
    10081 gacctgccgg gccgcggtcg cgccgggctg cttctcgagg cggtgctggt cctcgagtat
    10141 gaggccgccg accccgatgc gctccagcac ggcgacggtt cgcgccacct gcctggcatc
    10201 cccgtaacca tcatcgccgt cgaccaggca gggcatatct ggcacggcat ccaccacgtc
    10261 cctgatagcc ggcgccatct ctcccagcgc ggcaaggcca atatccggca gcgcgtagcg
    10321 ggcagccaag agggtggaac cgccgatgtt gattgccggg aagccggcag cctggatcat
    10381 tcgggcgctc agcgcgtcat gggcaagagg cgcgacgagc ggcctcgcgg ccctgtgcgc
    10441 ttgcctgagc ctggtcccgg gcccaacggc cttagccggt gtctccgacc tcgatgcaga
    10501 cggggaagct tcgttgttca aggtcaatgg aacccacctg agacgttatg ccgggaggtg
    10561 cgggccggtt cgatgccgaa tcatcggata ggccggcacc ttgagagccg gtggaacggg
    10621 tctgcgttgt ttttgggact gagaccgaac caaatcaatt tcaggctttt cgcatccccc
    10681 ctcccgtttt tccgggacat accaaccata tcatatcgaa cgacaggccg aacagacggc
    10741 gttccccgac caaggcgggc cttcaccgat ccccgaatca agaccttatg aggagaccat
    10801 gatgcaagat ttgcagacac atggaatcag tctgccacgg cttggcctcg gcacctttcg
    10861 catggcgaac gacgtctgcc agccggccgt cgagagcgca ctggccctgg gctaccgcca
    10921 catcgacacg gccgagatgt acggcaacga agaggctgtc ggggctgccg tcgccgcttc
    10981 gggaatcgct cgcggcgcgc ttcacgtcac cactaaggtc tggcacgaga atctggcgcc
    11041 cggagcgatt cggcgcgcgc tcgacgccag cctcaatagg ctgaagctcg actatgtcga
    11101 tctctatctc gtgcactggc cgtcgcggac gatggacctg ccgacgatct tcgagacgct
    11161 ctgccgcttg caggcggaag ggcgcacccg ggcgatcgga gtcgccaatt tcacggtgaa
    11221 tctgttgcgc accgccatcg aggacatcgg cgcgccgatc gcctgcaatc agatcgaata
    11281 ccacgtgttc ctcgatcaga cgccggtccg gtcatatttg gcgagcaaat ccattcctct
    11341 cgtcgcctat actccgctgg cccggggaga ggtcgccgag agcccggaac ttcaggcgat
    11401 gggacgcaag catggggcgt cggcggcaca gatcgccctc aagtggctgc tcgaccagga
    11461 cggcgtcgcg gcgatcccga aagcgcagcg gaccgaaagc cagagggcga acctcgaagc
    11521 gctgaacctg gtcctcgacg acgaagaccg gcgcgtcatc gcggctttgc cgaagaaccg
    11581 tcgcctgatc agtccgccct acgcaccgac ctgggatccg cttccggcgg gggtctcggc
    11641 gatcagataa tcgagattca aaaccccgaa ggttgcgcag gccaatcgcc cttgcgccag
    11701 ctttcggcta tcccgtccag cggttcatac tcattgctgc ccggggtcgg ctttgtcggc
    11761 ggattaggcc aattgcaggt gtgcatcgat ttcccgctga taggcgtcga ccttgccctg
    11821 ctccacctcg gtgataccga cggggcgccc cagcttgccc gattcgaaca gagcatagac
    11881 gatcgcgaga gcccgccggc cgatttctcc ggtaacctcc ggcgcggccc gcgtggcgat
    11941 gcagctggca aattcgtggt gctcaagagc gatcagccgc caatccgcct cgtcgaaggg
    12001 catgtcgtag cgccacactc gttcgccgcc aaacagctgg gaggccaacg gcgataggcg
    12061 gtagtccggt gcgaaatcga gaatccgtcc gtccgaaatg gcttcggagc ccattcgcca
    12121 tagcgtaagc ggcttcccgt tgcgctcacc ttgcgattcg agcgaaccgc cgctgccata
    12181 aagaatgcgc ttgtga
//
LOCUS       DASVDU010000309         6337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1055296, whole genome shotgun sequence.
ACCESSION   DASVDU010000309 DASVDU010000000
VERSION     DASVDU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055296"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..476)
                     /locus_tag="VF226_20770"
     CDS             complement(15..476)
                     /locus_tag="VF226_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7076479.1"
                     /translation="MKSRLDYWKVSPQGFAALRHLNDYVEGCGLERSLLELPKTRASQ
                     INGCAYCLDMHTKDARAAGESEQRLYTLSAWRETPFFSERERAALAWTEAITKISEHP
                     IEDALFADLQQHFSEKEIVDLTLAIFTINGWNRLAIPFRTPAGSYKPRTSG"
     gene            complement(828..2438)
                     /locus_tag="VF226_20775"
     CDS             complement(828..2438)
                     /locus_tag="VF226_20775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX7076480.1"
                     /translation="MADETQAGAAYAASGATNPWLVALVVAFAAFMEVLDTTIVNVAL
                     PHVAGSLGVSVDEASWVVTTYLISNAAALVASPFFGRWLGRKTFFITCLALFTAASVA
                     CGFAWNLEVLLLSRILQGLGGGGMVPVSQSILAETFPPENRGQGFAMFGVAVVVAPVI
                     GPILGGWLTENWSWHWCFLINGPVGLIAIALIASTLRQSAAAVAEARRRLRQEGGFDL
                     VGFLLVATFLGSLEIMLDRGLIDDWFDSSFIVTVAIICCLAFALMVPWELSRRSPMID
                     LRMVGTRQFGSCFIIMLAAGGTLLATTQFLPLLVQNEFGYTAVWAGLILTPGGLVAMG
                     MMFVVGYLSGRIQPKYLIAIGTLIVAFSMHILTAVYGDLDFWFFAISRMVLSVGLPLV
                     FLPIITASYEGIAPDKTDQASALINSARNTGGSIGISLTANIVAHREQFHHSRLVENV
                     VPSNISFQDTVQQVMSYFIGRGSSPQEAQGQVIAWIGDQVQTQATFLAYVDAFWVLML
                     MSLAVIPLALMLRNTPLGTRRGAGSHPA"
     gene            complement(2438..3697)
                     /locus_tag="VF226_20780"
     CDS             complement(2438..3697)
                     /locus_tag="VF226_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX7076481.1"
                     /translation="MALEANNGNPRSKTPDEHPTGDISEQTAKGEHQAPRPVQEERET
                     PHEGFLRRHPFLSVIGLVLLAMMIAGGYLYWDHSAHFESTDDAFIASRPFSIAPKVSG
                     YITEVPVTDNEHVPAGGVIARIDDREFRIALEQAEAQVASAEAGIQNVDAQLEVQQAE
                     IASLKAQVDKAQAALDLARVTWERDRPLVEKGWATAQQGTIDVQNVKTQEATVKSAQA
                     DLELAQRQLDSLKAQRKTAVSNLEQARALRDQAKLNLSYTTITADQPGHVVQLSAAVG
                     GLAQPGTAIAMFVPDEIWVAANFKETQLDAIRPGQPVTIQIDAYPERRIAGRVVSVQP
                     GSGTAFSLLPPQNATGNFVKIVQRVPVKIIMENPPTDVALGPGMSVQPTVRIDPEPSF
                     YEQLLSDQIPSIRDHLASLTEWLTGWF"
     gene            4569..5987
                     /locus_tag="VF226_20785"
     CDS             4569..5987
                     /locus_tag="VF226_20785"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7076482.1"
                     /translation="MLPMFLAVMDQTIVATALPAIAGELGEVERTSWIVVSYLIAATI
                     AAPIYGRLGDSFGRRRMMLVALSVFVVASLLCAAAPSTTLLSAARFLQGLGGGGLMTL
                     SQALIGEAIPPRERARYQGYLAAIAVTANTFGPVVGGFLTEHFGWRSIFFISVPIGSA
                     ALALTLRLPTRVAAERLKWRADPGGLLFFAVFVATTLLALEQVQQVKLSALPLGAGLL
                     SVGIIALVLLVWQEKRAESPLLPLSILRMPAIWKSDALAACHGGALVSLITFLPVYLQ
                     VVRGASPAESGFLLVPLTVGIGAGSLVTGRLVSRTGRTTIFPVIGLALLTFNILCLAL
                     FAEQLSTGAFVTVLLFNGLFMGTVMGVVQVLVQSAAGIRRLGEAAASVQLSRSLGAAF
                     GTALVGTVLFAFLQIRNPEAARVFSMLFEHATYAVPAMSPEQRAALQTDIADAFRAAF
                     LLIAGFTTCGFFLALTNPLRRI"
     gene            complement(6112..>6337)
                     /locus_tag="VF226_20790"
     CDS             complement(6112..>6337)
                     /locus_tag="VF226_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076483.1"
                     /translation="WFRILQFAADQKTTPEPSTELTGAGEARRRGARDGAQTFRTSGK
                     DPRRSGPLASMNAQELSEIQDLSSSKVNRA"
BASE COUNT         1203 a         1950 c         1926 g         1258 t
ORIGIN      
        1 gccgcgaacc agcctcagcc gctggtgcgc ggtttatagc tgcccgcagg cgttcggaaa
       61 gggatggcca atctgttcca gccattgatg gtgaagattg ccaacgtcag atcgactatt
      121 tccttttccg aaaaatgctg ctgaaggtcc gcgaagaggg catcttcgat gggatgctcg
      181 gagatcttgg taatcgcctc ggtccaggca agtgccgcgc gctcgcgctc gctgaagaac
      241 ggagtctcac gccaagctga cagcgtgtag agccgttgct ccgactcgcc cgccgcgcga
      301 gcatccttgg tgtgcatatc gaggcagtag gcgcatccgt tgatctggga ggcgcgagtc
      361 ttgggaagct cgagcagcga acgctcaagg ccgcatccct cgacgtagtc attcagatga
      421 cggagcgccg cgaagccctg tggcgatacc ttccaatagt ccagtctcga tttcattatg
      481 ctcctccgtt cgtttcctgc aggctggggc ctgtcgacct cgatagctat agggtcgctg
      541 gccggaaagg tctcggcgag cgctctatcc agaagttcat cgagatggga tcgcgaagag
      601 gtgttgctgg cgagtggctc catcctgctc ttgtcgtcga gcatccgcac ccttgtaggt
      661 tctgtaagct gaagcggcta ctccgccgca tgcatataga cccattcgcc tgcccgtagt
      721 tccaatgtgg aaccaacgag tagcgcgccg ctccgggcaa tgcgcctccg ccccggttcg
      781 gcaaacgcaa gtgccatcaa gatcgacata ggcgccagga ggcctgctca agcagggtga
      841 gaaccggcac ctcttctggt gccaagcggg gtattccgca acataagcgc caggggaatg
      901 accgcaagcg acatcaacat gagcacccag aaggcgtcga cgtaagcgag gaatgttgcc
      961 tgtgtttgga cctgatctcc gatccaggct atcacctggc cctgcgcttc ctgaggcgag
     1021 ctgccgcggc cgatgaagta gctcatgacc tgttgaacgg tgtcctgaaa ggagatgttc
     1081 gacggcacca cgttctcgac gagccggctg tggtgaaatt gctcgcgatg cgccactatg
     1141 tttgcagtga gggaaattcc gatcgacccg ccggtattgc gagctgagtt gatcagcgcc
     1201 gaggcctggt cggtcttgtc aggcgcgatg ccttcgtacg atgccgttat gatcggcaga
     1261 aagaccaatg gcagcccgac gctcaaaacc atccgcgaaa tggcaaagaa ccagaaatcg
     1321 agatcaccgt acacggccgt gaggatatgc atcgagaacg cgacgatgag tgtgccaatc
     1381 gcaatcagat atttcggctg gatccttccc gacaaatagc ccacgacgaa catcatgccc
     1441 atggcgacca agccacctgg cgtcagaatg aggccggccc acacggccgt atagccgaac
     1501 tcattctgga ccagtagcgg cagaaactgt gtcgtagcca gcaaggtacc tccagcggcc
     1561 aacatgatta taaagcagga gccgaattgc cgggtcccca ccattcgcaa atcgatcatc
     1621 ggactgcggc ggctgagctc ccaagggacc atgagcgcaa acgcgagaca gcagatgatt
     1681 gcgaccgtga cgatgaagct cgagtcgaac cagtcgtcga tgagcccacg atcgagcatg
     1741 atctcgagcg agccgagaaa ggtcgccacg agcaagaagc caaccagatc gaacccaccc
     1801 tcttgccgca gacgccgcct cgcctccgcg acggctgccg ccgactggcg tagggtcgat
     1861 gcaatcagcg caatcgcgat gaggccgaca ggtccattta tgagaaagca ccaatgccag
     1921 gaccagtttt cagtgagcca gccgccgagt atcggaccga tcaccggggc aacgaccacg
     1981 gccacaccga acatcgcgaa gccctggcct cggttttccg gcggaaaggt ctccgccaga
     2041 atcgattggg agaccggtac catgccgccg ccgccgagcc cctgcaggat gcgcgacagg
     2101 agcagtacct ctagattcca ggcaaagccg caggccacag aagccgcggt gaacagcgcc
     2161 agacatgtga tgaaaaaggt tttgcggcca agccaacggc caaaaaaggg actggccacc
     2221 aaagctgcgg catttgaaat cagataagtg gtcacaaccc aggaagcctc gtccacgctc
     2281 accccgaggc tgccggcgac atggggcagc gccacattga cgatggtggt atcaagcacc
     2341 tccatgaatg cagcgaacgc gaccaccaga gcgacgagcc agggattcgt cgcgccactc
     2401 gctgcgtaag ctgctcctgc ctgagtctcg tcagccatca aaaccacccc gtcagccact
     2461 cggtcaggga cgcaagatga tcgcggatcg acggaatttg atcggaaagg agttgctcgt
     2521 agaacgacgg ctcagggtca attcgcacgg ttggttggac tgacatgccg ggacccaagg
     2581 cgacgtcggt cggcggattt tccattatga ttttcacggg aacgcgctgg acgatcttga
     2641 cgaaattgcc ggtggcgttc tggggcggca atagcgagaa cgctgtgccg gagccaggct
     2701 ggacacttac gacccggccg gcaatccggc gctccgggta ggcatcgatc tggattgtca
     2761 caggctggcc aggccgtatg gcatcgagct gggtctcttt gaaattcgcc gccacccaga
     2821 tctcgtctgg cacaaacatg gcgatggccg tgcctggttg cgcgagcccg ccaactgcgg
     2881 cgctgagctg gacgacgtgc cccggctgat ctgccgtgat ggtggtgtag gataggttca
     2941 gtttagcctg atcacgcaaa gccctggctt gctccaggtt cgagaccgcg gtcttgcgtt
     3001 gcgcctttag ggagtcgagc tgccgctgcg ccagctcgag atctgcctgc gcactcttta
     3061 ccgtcgcctc ctgggtcttg acattctgga cgtcgatcgt gccctgctga gccgtggccc
     3121 aacccttttc gacgagtggc ctgtcgcgtt cccaagtgac ccgagccaga tcgagagcgg
     3181 cctgagcctt gtcgacctga gccttcaaag aggcaatctc cgcctgctga acctcgagct
     3241 gtgcatcaac gttctggatg cccgcctctg cgctcgcaac ctgcgcctcg gcctgctcga
     3301 gcgcgatgcg aaactcgcga tcatcgatgc gcgcgatcac tccgcccgcc gggacgtgct
     3361 cattgtcggt caccggcact tcggtgatgt atccggagac tttaggggca atggagaacg
     3421 ggcgggacgc gatgaacgcg tcgtcggtgc tctcgaagtg ggcgctgtgg tcccaataaa
     3481 ggtagccacc ggcaatcatc atggccagaa ggacgaggcc gatcaccgag aggaacggat
     3541 gccgccgcag gaatccctcg tgcggtgttt cccgttcctc ctggaccggt ctcggggcct
     3601 ggtgttcacc tttcgctgtc tgttccgaga tgtcacccgt ggggtgctca tccggagtct
     3661 tggatcgtgg attgccgttg tttgcctcca gggccactgt cgaaactcct gccagctgga
     3721 gtaaccacaa aaaatcgcaa cagtgccgtt tcgttccttg cctcgtccta ctgcgacacc
     3781 tcgggcccgg aacggcatgc gcacggaaaa gcgtgtggcg tctttcgtgc accttgcccg
     3841 cgatgcaagg gccgacttcg gagggattat ctgtccgata agcaatggat cgtcctcatc
     3901 tccgctggaa cggtgcaagc atcaatgcaa ctgcatcgat atcgaggaac accgtatggc
     3961 ggcagcgccg gaccgaccga cggctccagc gccgaaagta catgtcacct aggccaaccg
     4021 catgatctat gacttccaat gcgccgcggt gccggccctg taggcagctg gcggcctgtg
     4081 ctgagcgtct cgcgctcggc gccgctcagg ccgggggcgc aataaagtag gcctggaacg
     4141 gcagtaccgg cagctaggtc cggatgtctt cgccttgccg ctcttgagga tcgccgagca
     4201 ggtgaccgtg ttgacttgga ggaggccacc aggtcggcgc gtcagtagcc tctaacggcg
     4261 ctccacctgc gccggaacgg ggtcgatgcg gtggagttgt cgctcaagct gggccgcatc
     4321 cctgggctgt cacttctggt caagagtgcc gatgcgcagt cccgcttgag ccagatgatc
     4381 ttcgcatgcg caacgcgaca catcatgctc atgccatctt gccggtcctg atgacgcact
     4441 gctaaaccac cacgccggaa gtgatcccag cacggtagac attgtttgaa acacccgaac
     4501 aaaaccaaag ccccggagct ttcgggagca gaaaaccgca agtcttcctc gccatctttc
     4561 cctcgatcat gctgccgatg ttcctggcgg tcatggatca aaccattgtc gccacggcgc
     4621 tgccagcgat cgcaggggag ctgggcgagg tcgagcgcac gtcctggatc gtggtttcct
     4681 atctgatcgc ggcgacgatt gccgcgccga tttatgggcg gctcggcgat tcctttggcc
     4741 ggcggcgcat gatgctcgtc gccctcagcg tattcgtcgt cgcatccctg ctatgcgcgg
     4801 cggcgccctc gacgacactg ttgagtgccg cacgctttct gcaagggctg ggcggcggcg
     4861 ggttgatgac gctgtcccag gcactgatcg gcgaagcgat cccaccgcgc gagcgcgcgc
     4921 gctatcaggg gtatctggcc gcgatagccg tgaccgccaa tacgttcggc cccgtcgtgg
     4981 gtggctttct gacggagcac ttcggctggc gttcgatctt cttcatcagt gtgccgattg
     5041 gatcggctgc gctggccctg acgctgcggc taccgacgcg agtggcggcg gaacggttga
     5101 aatggcgcgc cgatcccggc ggtctcctgt ttttcgcagt gttcgtcgct acgacgctgc
     5161 tcgcactgga gcaggtgcaa caggtcaagc tgtctgctct gccgcttgga gcgggcctgc
     5221 tgtcggtcgg catcatcgct cttgtgctgc tggtctggca ggaaaagcgc gccgaatcgc
     5281 cgctccttcc cttaagcatc ctgcgcatgc ccgcaatctg gaagagcgat gcgctggccg
     5341 cctgccacgg cggcgcgctg gtttcgctga tcacgtttct gccggtctat ctgcaggtgg
     5401 tccgcggcgc ctcgcctgcc gagtccggct tcctgctggt gccgctcacc gtcggcatcg
     5461 gtgcgggctc gcttgttacg ggtcggctgg tcagcaggac ggggcggacc acgatctttc
     5521 ccgtcattgg cctggcgttg ctcaccttca acatcctgtg cctcgccctg ttcgccgagc
     5581 aattgagcac gggcgcgttc gtcaccgtac tgctgttcaa tggattgttc atgggtacgg
     5641 tgatgggcgt ggttcaggtc ctggtgcaga gcgcggccgg cattcgacgg ctcggcgagg
     5701 cggcagcttc ggttcagctc tcgcgctcgc tcggcgccgc cttcggcacg gcgctggtcg
     5761 gcaccgtgct gttcgccttt ctgcagatcc gcaatccgga agcggcgcgc gtcttctcca
     5821 tgctgttcga gcatgccacg tatgcggtgc cggcgatgag cccggagcag cgcgccgcgc
     5881 tccagacgga catcgctgac gccttccgcg ccgcgttcct gctgatcgcc gggttcacga
     5941 cctgcggttt tttcctcgcg ctgaccaacc cgttgcggag gatttgagcg catatttgcc
     6001 ccgcgaaaac cggcaaatcg cagaggcatc ccctgcatgg caggggcggc ctgcttccca
     6061 cttgcaggcg acacacaaat ggattcctca agagcctcac acgagtctct gctaggccct
     6121 gttcacttta gatgaactca aatcctgaat ttctgaaagt tcctgcgcat tcatcgaggc
     6181 caacggcccg gatcttctag gatcctttcc actggttcga aaggtttggg ctccatctcg
     6241 agctccgcga cgccgcgcct caccggcccc cgtgagttca gtagatggct ctggcgttgt
     6301 tttctgatcg gcagcaaatt gcagtattcg aaaccat
//
LOCUS       DASVDU010000310        26924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1283569, whole genome shotgun sequence.
ACCESSION   DASVDU010000310 DASVDU010000000
VERSION     DASVDU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26924
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283569"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VF226_20795"
     CDS             complement(<1..495)
                     /locus_tag="VF226_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333449.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HEX7076484.1"
                     /translation="MQQRTDEPVTALDLGPDAEPTPEIAAYFAKCKEKLGFVPNVLRA
                     YAFDNKKLQAFIDMVDDLMLGDSGLSKLEREMIAVAVSSVNHCHYCLTAHGAAVRQRS
                     GNPELGELIAQNYRAARLDKRQKAMLDFAVKLTEHPDKIEEADRKALRQAGFSDRDIW
                     DIAAI"
     gene            714..5531
                     /locus_tag="VF226_20800"
     CDS             714..5531
                     /locus_tag="VF226_20800"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX7076485.1"
                     /translation="MALHAPQTAGDLLSSIELHLDASAELKTYCGLLFGEHSDDLERY
                     APESLAAIARSNFSFAREKPGGRHAIRWQSVRLETITGTHPFTLVEIVNDDMPFLVDS
                     VLNELQARGLSALLVLHPILKVQRSDEGELERVLGPGDRNWGDGTQESWMTLLLDAMT
                     ADMASGLTEALSAVLDDVRVAVTDWHSMTARLERAIQALQSSPPSVAPGLLAESLAFC
                     HWLLDGQFTFLGLREYKLVGKEETSELVAVPKSGLGILREPNLHVLSGRGDPLGMTQE
                     ARRQFFKPHPLIITKSNIVSRVHRRVFMDYVGLKTYNPDGSLAGELRLVGLFTSHAYT
                     QPPQQIPFLRHKVETVMARSGFPAGSHDAKALANVLATFPRDELFQIDEQQLLDWARD
                     IVDLDLRPRVRVFARFERFDRFVSALVFVPRDRFSTSVRERIAAILSEAYEGHLQAFT
                     PFFTEGPLVRVHFIIERREGPRPKVSEHDLEARITEATRTWDDQLSEALHSGGEALAA
                     LAPKYQHAFPAGYTEMFSVTRAIDDIARIERLGPDRPLAIDFHAEGRTGGRKIRAAIY
                     RFDRPIPLSERVPVLENLGFFVIDERSYRVIPHFDGTAREVILHDMVLETADGSALDL
                     SSCDRRMEDCFIAVCRGEAENDGFNRLVITAGADWREVAALRAYATYLRQIGAPFGFR
                     YIADTLNRHAGMARDFIELFHTRFDPDRELTFSERAEAEELIRERIENALALVASLDE
                     DRILRYYANLIKATVRTNFFQRDSNGRRPATIAYKLASSLIEGLPEPKPYREIWVYSP
                     RVEGVHLRFAPIARGGIRWSDRAQDFRTEVLGLAKAQQVKNTVIVPQGAKGGFVPKLL
                     PRTGSREEIQKEGIAAYRAFVSSLLDITDNIVAGKIVPPDRVVRHDGDDPYLVVAADK
                     GTASFSDIANEISLDHDFWLGDAFASGGSSGYDHKRMAITARGAWECVKRHFREMDID
                     IETQPIRVVGVGDMSGDVFGNGMLLSRAIKLVAAFDHRDIFIDPDPDPEISYAERKRL
                     FDLPRSSWQDYDKSLISRGGGVFSRSAKSIPLSPEMRELLGVDAEAMTPTALMNAILK
                     CETDLLWFGGIGTFVRASSETPEQVGDRANDAIRVTGQEVRAKVIGEGANLGVTQLGR
                     TEFARKGGRINTDFIDNSAGVNSSDLEVNIKIALGPAVASGRLDPEDRRELLASMTED
                     VAAACLVNNYQQSLALSLAERSAARDIAYLSRLMRALEKRGLLDRKLHGLPSRQEMAQ
                     REATGSGLTRPELAVLLSFAKIALSEDLIHSSVPDDPVCEPLLTNYFPEALRHSFLED
                     IKAHRLRREIIVTGLTNSMLNRGGPAMAVRLADESGRGPDDVAAAFLAVSAIFQLPDL
                     WRAVDQLDGKMPGQAQLGLYARIQDFLLEQSANMLRIGMGEGLAPTIATHRAGVEELA
                     AAFEDCLTPHQHEHLATLREALQQAGATPEVAARIAALDILSHAMIITQLAHATDRTI
                     AEAARIAFAASSYFRLDDLKELAASLQLRDYYDRLAINGAVHTLDAARRALSREILSA
                     SKKGAADFAAWESENGGRLARAKAALDEMATTGDVTVSRLTVAASQVRELTSG"
     gene            complement(5675..6166)
                     /locus_tag="VF226_20805"
     CDS             complement(5675..6166)
                     /locus_tag="VF226_20805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7076486.1"
                     /translation="MALRPATHGDWPLIRSWLARPDIQRWWGPQASTEAEVLMALDSA
                     HAICRMIEVEGEPVGYAHAVDATLWGESLPDDLSPGTWDLDIFIAAEAHRGKGVGVAA
                     LELLKQEVFETTLAIAVCVFASIENEAAVRAYEKAGFRWRRVWHDPVSGPEWFMIAER
                     PTR"
     gene            complement(6198..7784)
                     /gene="purH"
                     /locus_tag="VF226_20810"
     CDS             complement(6198..7784)
                     /gene="purH"
                     /locus_tag="VF226_20810"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX7076487.1"
                     /translation="MSDPVIRRALLSVSDKTGLEELARHLHESGVELISTGGTAAALK
                     RAGLPVKDVSEHTGFPEMMDGRLKTLHPKVHGGLLAIRGRPDHDRAAEEHGIAPIDLL
                     VVNLYPFEATVARGASPEECVENIDIGGPAMIRAAAKNHASVAVLVDPEDYPRIIETM
                     RNLGGATSLELRKSLAAKAFARTAAYDAAISGWFARELGDTAPAWRAFAGKLAQPLRY
                     GENPHQRAAFYRGPDHRPGVATATQHQGKELSYNNLNDTDAAFELVAEFDPAETAAVA
                     IIKHANPCGVACAATLKEAYLAALRCDPVSAFGGIIALNRPIDAEAAEEITRIFTEVV
                     IAPDVTPEAKALFSAKKNLRLLTTGALPDPRAPGVAFRSVAGGFLVQSRDAASVDDME
                     LKVVTKRQPTEQELADLKFAFRVAKHVKSNTIVYAKNCATVGIGAGQMSRVDSARIAA
                     LKAEEASRAAGLAESLAKGSVVASDAFFPFADGLIAAAEAGATAVIQPGGSVRDDEVI
                     KAADERGLAMVFTGIRHFRH"
     gene            complement(7897..9597)
                     /locus_tag="VF226_20815"
     CDS             complement(7897..9597)
                     /locus_tag="VF226_20815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX7076488.1"
                     /translation="MSLTLAEQTRLARLATDRVRRAALARLLSSRLLRWRYGAPVADE
                     LLIVPQELRTADPSFADEIAIGQFGLGGAVAYIGDGSPFDLKPPSLEWARELHGFGWL
                     RHLRAAETEEAQEQAIDLVTEWIRKHGSRGGIAWEPPVIGRRLISWICSASLLLDGVD
                     QATYDRIADSLGDQLIRLSATWRDAPAGYPRLLSLTALVFGDLCIAGHDQHLREIQDA
                     FAEELETQILPDGGHISRNPGVPVELLLDFLPLRQCFSVRERPVPPALDTAIERMMPH
                     LRFMRLGDGSLARFNGMGAPNLDLVATVLAYGDPPSSLPVHAPQSGYARLACGDVVVI
                     ADVGSPPPLELAGQAHAGCLSFEMSAGKCPVFVNCGAPGAADQDWRAASRATAGHNTL
                     CLGGKSSSRLVLHDLLETLVGSAPIRFPSRVSARVRHLADGGISLTARHDGYHRQFGL
                     IHRRSLVLDPDGNKLTGTDSLGPLRGSLRLKTDLPFAIHFHLHPEVGCNGASRGGPVE
                     IVLPNGERWAFTAKGAHVSIEDSFHCADLSGPRRALQIVLRGACYGESEVRWEVERRG
                     "
     gene            complement(9791..11164)
                     /locus_tag="VF226_20820"
     CDS             complement(9791..11164)
                     /locus_tag="VF226_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7076489.1"
                     /translation="MAAGQRRTKERQTSEPRQCPPGLLARELAVELIAGVLDRGRTFD
                     EALAYGMARQEYRELAGNDRGLARLIAATVLRRHGQLTNIIGTFIERPLPRDRGRLTS
                     ILLAAAAQLVFLGSAPHAVISLAVEQARRDPRARRFDRLTNAVLRRVSERGAEIAAAQ
                     DPVRLNVPDWLRNRWSAAYGEATARLIGEASLREAPLDITAKSEPAVWAKRLGGILLP
                     TGSVRLRAEGRIENLDGFDEGAWWVQDAAAALPAKLVGNVEGQQIADLCAAPGGKTAE
                     LAAAGGHVSAVEVDERRIERLKANLARLRLEAEVVHADAAEWNPGRTFDAILLDAPCL
                     STGTIRRHPDILHLKRESDIDRLCAGQARLLANAARLVKPGGLIVYCTCSLEPEEGPQ
                     QIAQFLARTGEAFERVPIEPGEAGIAQEWITPDGDLRTFPFHLRLDDQAMSGMDGFYA
                     ARLTRRC"
     gene            complement(11171..12091)
                     /gene="htpX"
                     /locus_tag="VF226_20825"
     CDS             complement(11171..12091)
                     /gene="htpX"
                     /locus_tag="VF226_20825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX7076490.1"
                     /translation="MTSNYIKTGVLLALMTAILVSLGGLVGGKTGLVIAFIIALGMNL
                     FSLWNSDRMVLRMYGAEEVDQRTAPEFYALVRDLADRAGLPMPRVYIMHSDQPNAFAT
                     GRSPSRAAVCASTGLLNMLDREEVAGVIAHELAHIRNRDTLIMTVAATVAGAITMFAN
                     MMQFSMLFGGRRDGEGGGPLGIIGALAAIILAPLAAMLVQMAISRSREYEADRIGAHI
                     CGEPLWLASALQKIDHYARRIMNPVAEEAPTTAHLFIINPLSGQGMDNLFSTHPNTEN
                     RIAELQRLAAEMGRGASLRSASGPARGPWG"
     gene            12210..12404
                     /locus_tag="VF226_20830"
     CDS             12210..12404
                     /locus_tag="VF226_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEX7076491.1"
                     /translation="MTDQTEPTPPKRRLTPAAERALKEAEERRRQAGSDQRPQEIGGR
                     KGPDPVRYGDWEKGGIASDF"
     gene            complement(13195..13851)
                     /locus_tag="VF226_20835"
     CDS             complement(13195..13851)
                     /locus_tag="VF226_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX7076492.1"
                     /translation="MQYAAYVFDAYGTLFDVHSAVRRHAQAIGPDAQKLSEIWRTKQL
                     EYSWVRTLMGAYADFWQLTEQALDYAFDCVPSANRALRADLLHAYQQLDCYPEVPSVL
                     ESLKKAGARLAILSNGSPDMLDAAVRSAGLESVLDEVFSVDQVKRFKTDSTTYGIVTG
                     AFRLPPDAISFQSSNRWDIAGATRAGFRTVWINRFAAPNEYLDLPPARVLTSLEELQG
                     "
     gene            complement(14266..14402)
                     /locus_tag="VF226_20840"
                     /pseudo
     CDS             complement(14266..14402)
                     /locus_tag="VF226_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551728.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(14792..15268)
                     /locus_tag="VF226_20845"
     CDS             complement(14792..15268)
                     /locus_tag="VF226_20845"
                     /inference="COORDINATES: protein motif:HMM:NF037707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7076493.1"
                     /translation="MAALLGTSDENAQRFLVCPVALHDVGKFSRDFQAKCPDLWAETA
                     GPQLGDWRPSPPMSRHDQDGYAVRELLGLRELLRPATEGWSATTFNSLWAAVAGHHGQ
                     PRTRKMGGRNDAAGALMRNVGAAPERFKILRFGVGIAHRSTNVEGCTALDGRAIGG"
     gene            complement(15744..16220)
                     /locus_tag="VF226_20850"
     CDS             complement(15744..16220)
                     /locus_tag="VF226_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076494.1"
                     /translation="MNRFAALISREAQRITGHCCLVVQCEGVVDHPTTLAEQKGPEKT
                     VLERLKLDGRIAVVTGGAAGLDQVSALALAEAGAKVAIIDRAARNNWYAMWLARPILG
                     SSAVGDQEQRRHMRHDAVARRARVCELHFTEAIDVNTTPRIEPARPFEEPGGNEHG"
     gene            complement(16217..16924)
                     /locus_tag="VF226_20855"
     CDS             complement(16217..16924)
                     /locus_tag="VF226_20855"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEX7076495.1"
                     /translation="MLQGIDEAVEYVSSFAGEPRGHIKISAGIGFGVNILSELLPVFL
                     DRYPKIRLTLDLSTRLADMVTEGVDCAIRFGALPDSTLVSTQLGTMHRYLCASPAYLE
                     RRGTPVSIGDLDHHDTIEMLGADGRTLAWEFRKDDETRRIIPRPRVHLDEVLTIYRLI
                     RNGAGIGVVSAYICGPDIAEGRLIRLFPDWSMPSLPVSLVYPSRRELAPAVRAFADFM
                     KEAGTRGQAWRIDPLME"
     gene            17309..18394
                     /locus_tag="VF226_20860"
     CDS             17309..18394
                     /locus_tag="VF226_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX7076496.1"
                     /translation="MTDLFTPFELHGTTLSNRAVMAPMTRTRATEDGVPTALMRDYYA
                     QRTDAGLIITECTQISDQAHGIIRAPGIHRDEQIDGWRMITKSVHDKGSRIFNQIWHP
                     GRVSHPEILGGDAPVAPSAIPAEGDFFLPSGRVPFTVPRALRLEEIPAIVADFARATR
                     NARDAGFDGIEIHGANGYLLQQFLEDGSNTRSDAYGGSIENRARFTLEVVDAAIAQWD
                     AEHVGIRLSPAGVHYGMRDSDRPALYDYLIRELATRSIAYIHVTRPNKASYDAGPVAI
                     EDVAGFTRARFSGPIIVNGGYDREKAERVLVRGDADLVAFGVPFLTTPDLLTRMKVGA
                     ELNRPDPATFYGEGPVGYTDYPFLTPA"
     gene            complement(18832..19314)
                     /locus_tag="VF226_20865"
     CDS             complement(18832..19314)
                     /locus_tag="VF226_20865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HEX7076497.1"
                     /translation="MLYLLVKYLHVLGATVLLGTGAGIAFFMLLAHRTGDPAVVAGVA
                     RIVVIADFLFTATAVIAQPMTGVWLAHLAGYSLWEGWIALSIVLYIVTGAFWLPVVWM
                     QMRMRDLAIAAKQQGRALPPAYHRLFQIWFAFGFPAFAAVLAIFWLMIARPPVPWFDA
                     "
     gene            complement(19314..20600)
                     /locus_tag="VF226_20870"
     CDS             complement(19314..20600)
                     /locus_tag="VF226_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7076498.1"
                     /translation="MRVLLTGATGLVGSAVLAALSSKGHELVAIAHGQPPPAGLGAVQ
                     WARVDLARQDVESWLPLLDGVDAVVNCAGVLQDSPRDSTAAVHVKGPAALFEACLRKG
                     VRRIIHFSAIGVDRGALTAFSASKLAGDEKLMAMDLDWVILRPSVIVGGPVYGASALF
                     RGLAALPILPAMPETGPLQVVQLDDVTRTVLYFLRDDVPTRLALEIAGPQKLSFTEIV
                     LLYRRWLGWRAPYVVQLPSWAAAVAYRAGDLVAWLGWRPPIRSTARREISRGAVGDPS
                     PWMHLTNIIPRTLEASLLAEPASVQQRWFARLYLLKPLVIGIFALFWLATGLISVGPG
                     YATGVAYLNAGGITKWAGLSVIAGGLADMVIGIGVAIRRTARIALYAALLVTVFYLVA
                     GTLVLPSLWADPLGPMLKVWPILALNLVALAILEDR"
     gene            complement(20881..21597)
                     /locus_tag="VF226_20875"
     CDS             complement(20881..21597)
                     /locus_tag="VF226_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX7076499.1"
                     /translation="MLDKSNNIRKTAKVAARWQPVYDSLREAITSRRLAPGTKLPEDE
                     LASIYSVSRTVVRSALQALAHDRVVRLEPNRGAFVAQPSIKEAREVFDARKLIEPRLA
                     ALAAKAARPEHIRHLRQHLEREREAIRLDQSSEAIRLSAHFHITLAEIADHSIFTEII
                     RDLVSRSSLIIALYWRHRDTTCKCDAHRDLVDAIADGNAAEASALMKRHIADLLSGLD
                     LTRTEKKQERLADILGRPKS"
     gene            complement(21678..22406)
                     /locus_tag="VF226_20880"
     CDS             complement(21678..22406)
                     /locus_tag="VF226_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7076500.1"
                     /translation="MRILVINPNTTPSMTAKIGAAARSAAASGTEITAVNPADGPPSI
                     EGYFDEVFAIPGLIGEMQKAPDMDAYVIACFDDTGLDAARCLTPAPVIGIGEAAFHVA
                     SLVAGKFSVITTLARSIPAIEHNLVRYGLVSRCARVRAADVPVLDLEVAGSDAQSKIS
                     TEIGRAVAEDRAEAIVLGCAGMTDLAERLSADHGLPVIDGVACAVKLAENLVDLGLRT
                     SRIGGYAPPRAKRFSGHFSRFSPG"
     gene            complement(22410..23132)
                     /locus_tag="VF226_20885"
     CDS             complement(22410..23132)
                     /locus_tag="VF226_20885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076501.1"
                     /translation="MTDLLTLNDVELYYDHVYALKGVSLSVSEGETVALIGANGAGKS
                     SILRAITGLRRIRSGEIHYQGKRIDGLPSDEIVKLGISMVPEGRRVFPYMSVRDNLLM
                     GAFTRTDKAEIGRSLELVLTRFPRLKERYSQAAGTMSGGEQQMLVIGRALMSKPRLLL
                     LDEPSLGIAPKLVQDIARSIVAINRDDKVSVLLVEQNSRMALRISQRAYALSTGRVAL
                     SGASADLLSDERVRKLYLGAEL"
     gene            complement(23135..23914)
                     /locus_tag="VF226_20890"
     CDS             complement(23135..23914)
                     /locus_tag="VF226_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076502.1"
                     /translation="MPDILEISGLTKRFGGLVANNDISFTVKEREILSVIGPNGAGKS
                     TLFKLIASFISPTAGEVRFRGQRISGLAPHKVARMGVVRTFQETTIFKTMTVRDNVII
                     AHHLRSRANLAGFFFGTRAAQQDEAEFARSTDEIIDFLGLGGYRHELASNLPHGHLRA
                     LGIAIGLATNPQVLLLDEPFAGMNHEETRRAIEIVRGVRERGVTVLLVEHDMPAVMNI
                     SDRIVVLNFGQKIAEGTPQEIQENPTVIEAYLGAEDEALGI"
     gene            complement(23918..24892)
                     /locus_tag="VF226_20895"
     CDS             complement(23918..24892)
                     /locus_tag="VF226_20895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076503.1"
                     /translation="MTTQQKIASALAFLAVLLVGVPLLISATGRSDLYYTLTSVALLS
                     IISAGVWLTFYIGRINIGQGAFALMGGYVSAILVTQYGASFWLTLPIAGLLCAAASVL
                     IGLPILRLRGVYFAMVTLTLSEVARLTALAVPITNGAKGIVNIPLPSGLTIFGITLIP
                     DFATLANPRLAFYVTSVLLMALCYLALYRLVNSRIGRICQSLQQNEELASSIGVNISY
                     IRILAFAISSFLGGIGGAMFAAITQSMYPSSFGVADSINFMLNCFLGGLGYVLGPMLG
                     TFVLYFGWDLLFQTGVFQLLIYSSILIILMLVLPNGLLSIRLARQKQS"
     gene            complement(24897..25763)
                     /locus_tag="VF226_20900"
     CDS             complement(24897..25763)
                     /locus_tag="VF226_20900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076504.1"
                     /translation="MEQVIANGLYLGAQYALIALGLTLIFALMNVLNFAHGQMYVLGG
                     FVTYTVYGQLGLPFPVAVLCSGVTLAAVGAIIEKFLFGPVIKRSAREESTMLLAAAIA
                     FFLDAIILLLFGEKQRGVPKIINGVFVSDIIIMPYDRIAVGVVAAAMIAAFVLYMQYS
                     RPGRAMRALAQDRVAAQLMGVNVDRYSMIGFALGAMLAGLVGGLLVSITGVNSGIGGP
                     ISIKAFLMVMIGGAGVVGGAIAGGFILGMMESVGLTLLRNFGDVTYLVIFVLLMIFLS
                     IRPNGLMGKPWG"
     gene            complement(25835..>26924)
                     /locus_tag="VF226_20905"
     CDS             complement(25835..>26924)
                     /locus_tag="VF226_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076505.1"
                     /translation="IGFVGVTSGPAAAWGISNVRSMQTRADWLNEKGGVEIGGETYKI
                     TVVTFDDQKDPRRAIAGMEKMAQEGVRYVVGPNVDDGAAAVRPVAESKGIVYFPYAFP
                     KELYAPPASNAVLGMVASYQSAPAIYKYLKENKGIKTVAFVAANESDPLSQRDAGVAA
                     AKQLGLEVVAQNDTYQTDTRDFTPVLTPVVRLKPDLLVLSGVSPGNAPLLIRAARELG
                     YEGLMSAETAHDATVLQEGAGDLANGFISVGGASTPEIRTAEMEEFIERYTKKYGEYN
                     DESNTKVYALEYIIETLKANPKAIDDVEEFKKTMETFSAPNPFVKDGTLKYVGMTSFG
                     QKRQLSVPMVVNEFRDGKFETLFVGEVD"
BASE COUNT         5083 a         8735 c         8414 g         4692 t
ORIGIN      
        1 tatggcagca atgtcccaga tgtcacgatc gctgaacccg gcctgacgca aagccttccg
       61 atctgcttcc tcgatcttgt ccggatgctc ggtgagcttt acggcaaaat cgagcatcgc
      121 cttctggcgc ttgtcgaggc gggcggcgcg atagttctgt gcaatcagct cgcccagctc
      181 cggatttccg gaccgctggc gcaccgccgc tccatgcgcc gtcaggcagt agtggcagtg
      241 attgaccgag ctcaccgcga ccgcgatcat ttcgcgttcg agcttgctga gaccgctgtc
      301 acccagcatc aggtcatcga ccatgtcgat gaaggcctgc agcttcttgt tgtcgaatgc
      361 gtaggcacgc agcacgttcg gcacgaagcc gagcttctcc ttgcacttcg cgaagtacgc
      421 ggcgatttcc ggcgtcggct cggcatccgg cccgaggtcg agcgcggtaa ccggttcatc
      481 cgttcgttgc tgcatggatc tcatcctcct cgccgacgta cggcgctcca ggtcttgatt
      541 gcaaagctcc ctggagttcg ccgtcaggct ccaaaatttc gcgatcattg cacgagaggg
      601 tagcgctgga gcgctgtcct gtcattcggc acaagccagc cactatatta tgttctagcc
      661 gccccagact ggaggatccg gcctgcaacg ttgatgatcg gagctctcat tccatggcac
      721 ttcacgcgcc acaaaccgct ggcgacctcc tgagcagcat cgagctgcac ctcgacgctt
      781 ccgccgagct caaaacctac tgcggcctgc tcttcgggga gcattccgac gacctcgagc
      841 ggtacgcccc ggagtcgctg gcagctatcg cgcggtcgaa cttctccttt gcacgggaga
      901 agccgggcgg gaggcacgcc atccggtggc aatcggttcg cctggagacg atcaccggca
      961 cgcacccgtt cacgctcgtc gagatcgtga acgacgacat gccgttcctc gtcgacagcg
     1021 tcctcaacga gctgcaggcg cgagggctct cggcactgct cgtcctgcat ccgatcctca
     1081 aagtgcagcg ctccgacgaa ggagagctgg aacgcgtcct cgggccgggc gatcgcaact
     1141 ggggcgatgg cacgcaggaa agctggatga cgctcctgct cgacgcgatg acggccgata
     1201 tggcgagcgg cctcaccgaa gcgctgtccg ccgtgctgga cgacgtccgt gtcgcggtga
     1261 cagactggca ctcgatgacg gcccgcttag agcgggccat ccaggccctc cagtcgtcac
     1321 ctccatctgt cgcgccaggg ctgctcgccg aatcgctcgc cttctgccac tggctgctcg
     1381 acggtcaatt cacgtttctt ggcctccgtg agtacaagct cgtcggcaaa gaggagacca
     1441 gcgagctcgt tgcggtcccg aaatccgggc ttgggatttt gagggagccg aacctacacg
     1501 tcctcagcgg ccgtggtgac ccgctcggga tgacgcagga ggcgcgccgg caattcttca
     1561 agccccatcc gctcatcatc acgaagtcca acatcgtatc gcgcgtccac aggcgcgtgt
     1621 tcatggatta cgttggcctc aagacctaca atccggatgg ctccttggcc ggtgagttgc
     1681 ggctcgtcgg gctcttcacg tcccacgcct acacacagcc gccgcagcag atcccttttc
     1741 tcaggcacaa ggtcgaaacc gtcatggcgc gctcgggctt tccggcgggc agccacgacg
     1801 ccaaggccct cgccaatgtg ctcgccactt tcccgcgcga cgagctgttt cagatcgacg
     1861 agcagcagct gctcgactgg gcgcgggaca tcgtcgatct cgacctgcgg ccgcgcgtac
     1921 gcgtgttcgc acgcttcgag cgcttcgatc gcttcgtctc cgccctcgtg ttcgtgccgc
     1981 gcgaccgctt ctccacgagc gtccgggagc ggatcgctgc catcctgtcc gaagcctacg
     2041 aggggcacct gcaggcgttc acgccctttt tcaccgaagg cccactcgtt cgcgtccatt
     2101 tcatcatcga gcggcgggaa ggcccacgcc cgaaggtctc ggagcacgac ctcgaggcac
     2161 gcatcacgga ggcgacgcgc acctgggacg atcagctttc cgaagcgctc cactccggcg
     2221 gtgaagcgct ggcagccctc gccccgaaat accagcatgc tttcccggcc ggctacacgg
     2281 agatgttcag cgtcacgcgg gccatcgacg acatcgcccg catcgagcgc ctgggtcccg
     2341 atcggccgct ggcgatcgac ttccatgcgg aggggcggac gggcgggcgc aagatacggg
     2401 cagccatata ccgcttcgat cggcctattc cgctatcgga gcgcgtgccc gtcctggaga
     2461 acctcgggtt cttcgtcatc gacgagcgca gctaccgcgt aataccgcat ttcgacggca
     2521 cggcccgcga agtcatcctg cacgacatgg tgctcgaaac ggctgatggt tcagctctcg
     2581 acctgtcgag ctgcgaccgt cgaatggaag actgcttcat cgccgtctgc cgcggggaag
     2641 cggaaaacga tggcttcaac cggctcgtaa tcactgccgg ggcggactgg cgcgaggtgg
     2701 ccgcactcag ggcgtacgcc acgtacctgc ggcagattgg ggcgccgttc ggattccgct
     2761 acatcgccga tacgttgaac cggcacgccg gcatggcgcg cgacttcatc gagctgttcc
     2821 atacgcgctt cgatccggac cgggagctca cgttcagcga gcgagccgag gccgaggagc
     2881 tgatccgaga gcgcatcgag aacgcactgg ctctcgtggc gagcctggat gaggaccgca
     2941 tcctgcgcta ctacgcaaac ctcatcaagg cgaccgtacg caccaatttc ttccagcgcg
     3001 acagcaacgg acgccggccc gccacgatcg cctacaagct ggcctcttca ttgatcgaag
     3061 ggctgcctga gccgaagcct tatcgggaga tctgggtcta cagtcccagg gtcgaagggg
     3121 ttcatctgcg cttcgcgccg atcgcgcgcg gcggcatccg ctggtccgac cgggcacagg
     3181 atttccggac ggaggtgctg gggctcgcca aggcccagca agtgaagaac acggtcatcg
     3241 ttccgcaagg cgccaaaggc ggcttcgtgc cgaagctgct tccgcggacg ggatcgcgcg
     3301 aggaaatcca gaaggaaggc atcgccgcct atcgcgcgtt cgtatcgagc ttgctcgaca
     3361 tcaccgacaa catcgttgcc ggtaagatcg tgcctccgga ccgcgtcgtg cgccacgacg
     3421 gtgacgaccc ctatctggtc gtcgctgccg acaaggggac ggcgagcttt tctgatatcg
     3481 cgaacgaaat ttcactggat catgatttct ggcttggcga tgccttcgcc tcgggcggct
     3541 cttcaggcta cgaccacaag cgcatggcga tcaccgcccg aggtgcgtgg gagtgcgtca
     3601 agcggcactt ccgcgaaatg gacatcgaca tcgagacgca gccaatccgc gtggtcggcg
     3661 tgggcgacat gtccggcgat gtgttcggga acggcatgct gctgtcacga gccatcaagc
     3721 tcgtcgctgc gttcgaccat cgcgatattt tcatcgatcc cgatccggac ccggaaatca
     3781 gctacgccga gcgcaagcgt ctgttcgacc tgccgcgatc gagctggcag gactacgaca
     3841 agtcgttgat ctcgcgcggc ggtggcgtgt tctcgcgctc cgccaagtcg atcccgctgt
     3901 cgccggaaat gcgcgagctg ctcggcgtcg atgcggaggc gatgacaccg accgccctga
     3961 tgaatgccat cctgaagtgc gaaacggatc tgctctggtt cggtgggatc gggacgttcg
     4021 tgcgcgcctc gtccgagacg ccggagcagg tgggcgatcg cgcaaacgat gcaatccgtg
     4081 tcaccgggca ggaggtgcgc gcaaaggtca tcggcgaagg tgccaatctc ggcgtcaccc
     4141 agctcggccg aaccgagttc gcgcgcaagg gcggccggat caacacggac ttcatcgaca
     4201 attcggcggg cgtcaactcc tccgatctcg aggtcaacat caagatcgca ctcgggccgg
     4261 ccgtggcgtc cggccggctc gatccggaag accgccgcga gctgctggcc tccatgaccg
     4321 aggacgtggc cgccgcatgc ctcgtcaata attatcagca gtcgctggcg ctgagcctgg
     4381 ccgaacgcag cgctgcgcgg gacatcgcct atctctcccg gctgatgcgg gcgctcgaaa
     4441 aacgcggcct gctcgaccgc aagctgcatg ggctgccgag ccggcaggag atggcgcagc
     4501 gggaggccac gggatcgggc ctcacacggc cggagctcgc tgtgctgttg agcttcgcca
     4561 agatcgcgct gtccgaggac ctgatccaca gctcggtgcc ggacgatccg gtctgcgaac
     4621 cgctgctgac gaactacttc ccggaggcgc tgcggcacag cttcctcgag gacatcaagg
     4681 cccaccgcct ccgccgcgag atcatcgtca ccggtcttac caattccatg ctcaatcggg
     4741 gcggaccggc catggccgtg cggcttgccg acgaaagcgg acgcgggccc gacgatgtgg
     4801 cggcggcctt ccttgcggtc agtgccatct ttcaactgcc cgacctgtgg cgcgcagtcg
     4861 atcagctcga cggcaagatg ccggggcagg cgcagctcgg gctctacgcg cggatccagg
     4921 acttcctgct ggagcagtcc gcgaacatgc tccgcatcgg catgggcgaa gggcttgcac
     4981 ccacgattgc aacgcatcgc gccggcgtcg aggagcttgc cgccgcgttc gaggattgcc
     5041 tcacgccgca tcagcatgag catctcgcta ctttgcgcga agcattgcag caggccgggg
     5101 caacgcccga ggtcgccgcg cgcatcgctg cgctcgacat cctgtcccat gcgatgatca
     5161 taacgcagct cgcccatgcg accgaccgga ccatcgccga agcggcgcgg atagcctttg
     5221 ccgccagcag ctatttccgc ctcgatgacc tgaaggagct cgcagccagc ctgcagctcc
     5281 gggattacta cgaccgtctg gcgataaacg gcgccgtcca cacactggac gcggcgcgcc
     5341 gagccttgtc ccgcgagatc ctgagcgcct cgaagaaggg cgctgccgat ttcgcggctt
     5401 gggagagcga gaacggcggg aggctggcgc gcgcgaaggc agcccttgac gaaatggcga
     5461 cgacaggcga cgtcacggtt tcccggctga cggtcgccgc atcgcaggtg cgtgaactga
     5521 cgtccgggtg agaacgaaaa gcgccgcaag cgggagacga cgcctttgac agcttcacag
     5581 ccggacgagg ccgctctccc ggcaggccca gacgtggccg cgcacgtgcg tgtgttcgcc
     5641 ggatgggtcc tcgtcgattg ggcgctgcag gccctcaccg ggtcgggcgc tcggcgatca
     5701 tgaaccattc agggccggaa acgggatcgt gccagactcg ccgccagcgg aacccggcct
     5761 tttcatacgc gcggacggcc gcttcattct cgatcgacgc aaagacacag acggcaatcg
     5821 ccagcgtcgt ctcgaatacc tcttgcttaa ggagctccag cgccgccacg ccaacgccct
     5881 tgccgcgatg agcctcggcg gcgatgaaga tgtcgagatc ccacgtgccg gggctcaggt
     5941 cgtcgggcaa cgactcgccc caaagggtcg cgtcgaccgc atgtgcgtaa ccgaccggct
     6001 ccccttctac ctcgatcatg cggcagatgg catgcgcgct gtcgagcgcc atcagcacct
     6061 cggcctcggt acttgcctgc ggaccccacc agcgctggat atccggccgg gcgagccagc
     6121 tgcggatgag gggccagtcg ccatgcgtgg cgggtcgcag cgccacacca gcattggccc
     6181 cctccccgcg catgagatca gtgccggaag tggcggatgc ccgtgaagac catggcgagt
     6241 ccgcgctcgt cggccgcctt gatcacctcg tcgtcgcgca cggagccgcc cggctggatg
     6301 accgcggtag ccccggcctc ggcggccgcg atgagaccat cggcaaacgg gaagaaggca
     6361 tcggaggcca ccacggagcc tttcgcaagc gactcggcaa gtccagcggc gcgcgatgct
     6421 tcctctgcct tcaacgccgc gatgcgcgcc gagtccaccc gactcatctg gcctgcgcca
     6481 attcctacgg tcgcgcagtt cttcgcgtag acgatcgtgt tcgacttgac gtgcttggcc
     6541 acccggaaag cgaacttgag gtcggcaagc tcctgctctg tcggctgccg tttcgtcacg
     6601 accttcagct ccatgtcgtc gacgctcgcc gcatcacgcg actgaaccaa gaagccaccc
     6661 gcgactgaac ggaacgccac ccccggcgcg cgggggtccg gcaaggcgcc ggtcgtcaac
     6721 agacgcaggt tcttcttggc ggagaacagt gccttcgcct caggcgtcac atccggcgcg
     6781 atgaccactt cggtgaagat gcgcgtgatc tcctccgccg cctccgcatc gatcggccgg
     6841 ttcagggcga tgataccgcc gaatgcgctc acgggatcac agcggagggc cgcgagatag
     6901 gcctccttga gcgttgccgc gcaggccacg ccacacgggt tggcatgctt gatgatggcg
     6961 acggctgccg tttccgcagg gtcgaactcg gccaccagct cgaaggcggc atcggtgtcg
     7021 ttcaggttgt tgtaggacag ctccttgccc tgatgctgcg tggcggtcgc cacgccgggg
     7081 cgatgatcgg gcccgcggta gaatgcggcc cgctggtgcg ggttctcgcc gtagcggagc
     7141 ggctgtgcga gcttgcctgc gaatgcgcgc caagctggcg ccgtgtcgcc cagctcacgg
     7201 gcgaaccagc cgctgatcgc cgcatcgtag gccgccgtgc gcgcgaatgc cttggctgcc
     7261 aacgatttgc gcagctccag gctggtggcg ccaccaagat tgcgcatcgt ttcgatgatg
     7321 cggggataat cctcgggatc gaccagcacg gccacgctgg cgtggttctt ggctgctgcc
     7381 cggatcatcg ccggcccgcc gatgtcgata ttctcgacgc attcctccgg gctcgcgcca
     7441 cgcgcaaccg tcgcctcgaa cggatacagg ttcaccacca gcaggtcgat cggcgcgatg
     7501 ccatgctctt cggcggcccg gtcgtggtct ggccgcccgc ggatggccag caggccccca
     7561 tgcaccttgg ggtgcaacgt cttgaggcgt ccgtccatca tctcggggaa gccggtgtgc
     7621 tccgagacat ccttcaccgg gaggcctgcc cgcttcagcg cggcagccgt gccgccggtg
     7681 gagatcagct ccacgccgct ttcgtggaga tgccgggcaa gctcctcgag cccggtcttg
     7741 tcggagacgg acaacaacgc gcggcggatt acgggatcgc tcatgtcaat cggtcctgaa
     7801 gggctgggcc tgcgccctga cgagaaaatg ccgccggccg cctaccacaa aacgcggctg
     7861 ccctcaaaat ggccgatcgc ccctaggaca gccgggtcag ccgcgccgct cgacttccca
     7921 ccgcacctcg ctctcgccgt agcaggcgcc acgcagcacg atctgcagcg cgcggcgcgg
     7981 accggagaga tcagcacagt ggaagctgtc ctcgatcgag acatgcgccc ccttggccgt
     8041 gaaggcccaa cgttccccgt tgggcaacac gatctcgact ggacctccgc gtgaggcgcc
     8101 attgcacccg acctccggat ggaggtggaa gtggatggca aacggcagat cggtcttcaa
     8161 ccgcaagctc ccgcggaggg gcccgagcga atccgttccc gtcaatttgt tgccgtccgg
     8221 atcgagcacc aggctgcgcc ggtggatcag cccgaattgt cggtgatacc cgtcatgccg
     8281 tgccgtgagc gaaattccgc catcggccaa atgcctgacg cgcgcgctga cccggctggg
     8341 aaaccggatc ggtgccgagc cgacgagggt ttcgaggagg tcatgcagaa ccagtctcga
     8401 cgacgacttg ccgccgaggc agagcgtgtt gtggcccgcc gtggcgcgcg aggcggcacg
     8461 ccagtcctgg tctgccgcgc cgggcgcgcc gcagttgacg aagaccggac atttccccgc
     8521 gctcatctcg aacgacaggc agcccgcgtg agcctgtccc gccagctcca gcggtggcgg
     8581 cgagccgacg tccgcaatga cgaccacgtc tccgcaagca agccgcgcgt agcccgactg
     8641 gggtgcgtgc accggcaggc tcgagggtgg atcaccgtag gcgagaaccg tggcaacaag
     8701 atccaggttg ggcgcgccca tgccattgaa gcgggcgagt gagccatcgc cgaggcgcat
     8761 gaaccgcagg tgcggcatca tccgctcgat cgcggtatcg agggccggcg gcaccgggcg
     8821 ctcgcggacg gagaaacact gccgcagcgg caggaagtcg aggagcaact ccaccgggac
     8881 gccggggttg cggctgatgt ggccgccatc cggcaggatc tgtgtctcga gctcctcggc
     8941 gaacgcgtcc tgaatctcac gcagatgctg gtcgtgcccc gcgatgcaga ggtcgccaaa
     9001 caccagcgcc gtcagggaga gcagccgcgg atagcccgcc ggtgcatctc gccacgtggc
     9061 ggagagacgg atcagctgat cgccgaggct gtctgcgatc cggtcgtagg tcgcctgatc
     9121 gacgccgtcg agcagcaggg atgcgctgca gatccaggag atgagcctgc ggccgatgac
     9181 tggcggctcc caggcaatgc cgccgcgtga gccgtgcttc cgaatccact ccgtgacgag
     9241 atcgatcgcc tgctcctggg cctcctccgt ctcggccgcc cgcaaatgcc tgagccagcc
     9301 gaacccgtgc agctcgcgcg cccactccag gctcggcggc ttgagatcga atggcgagcc
     9361 gtcgccgatg taggcgacag caccccccag cccgaactgc ccgatggcga tctcgtcggc
     9421 gaagctcgga tcagcggtcc tgagctcctg cggcacgatc agcagctcgt cggcaaccgg
     9481 cgctccgtac cgccagcgta ggagcctgga gctcagcaga cgcgcaagcg cggcccgccg
     9541 gacccggtcc gttgccaagc gagcaagacg tgtctgttca gcgagcgtga gcgacacgag
     9601 tgtctccggg ccggcaacgg ccagacattt gcaactaagc tctcggggca gatcgaagag
     9661 tgatcatgcc atccgatagc gccgttgcga gaggtgaaca agcaggccgc ggaaaggctg
     9721 tgcgccgtct gtgggcgatc tgtggatcgt cctgcgggcg tgcgctctgg cgcggatcag
     9781 ccgagcacgc tcaacagcgc cgcgtcagcc gcgccgcata gaagccgtcc atgccggaca
     9841 tcgcttggtc atccaggcgc agatggaacg ggaacgtccg caagtcccca tcgggcgtga
     9901 tccattcctg agcgataccc gcctcgcccg gctcgatcgg gacacgctcg aaggcctccc
     9961 cggtccgagc aaggaactgc gcgatctgct gcggtccctc ttctggctcc agcgagcagg
    10021 tgcagtagac gatgagcccg ccgggtttga cgagcctggc agcgttcgcc agcagccgcg
    10081 cctgcccggc gcatagccgg tcgatgtcgc tctcccgctt caggtgcagg atgtccggat
    10141 ggcggcggat ggttccggtc gagaggcacg gggcatcgag cagaatggca tcgaatgtcc
    10201 tgccagggtt ccattctgca gcgtcggcat ggacgacctc ggcctcgagt cgcaggcgtg
    10261 ccaggttggc cttcaggcgc tcgatccgcc tttcatccac ctccacggcc gacacgtgtc
    10321 cgcccgctgc cgccagttcc gcagtcttgc cgccgggagc cgcgcagaga tcggcgatct
    10381 gctggccctc gacgttgcca acgagctttg ccggcaatgc agcagcggca tcctgcaccc
    10441 accaagcacc ctcgtcgaaa ccgtccagat tctcgatgcg gccttcggcg cgcaggcgga
    10501 cggagcccgt cgggagaagg attccgccca ggcgctttgc ccacaccgct ggttcagact
    10561 tcgccgtgat gtcgagaggc gcctcgcgca acgaggcctc tccgatgagc cttgcagttg
    10621 cctctccgta agccgcgctc cagcggtttc gcagccagtc cggaacattc agccgcacgg
    10681 ggtcctgagc ggcagcaatc tccgcacctc gctcggaaac gcgccgcagg accgcgttgg
    10741 tcaggcgatc gaagcgacgt gcacgcggat cgcggcgggc ctgctcgacg gccaggctga
    10801 tcacagcatg cggagcgcta cccaggaaca cgagctgcgc cgctgccgcc agaagaatgg
    10861 acgtcagccg tccgcggtcc cgcggcagcg gccgctcgat gaaggtgcca atgatattcg
    10921 tgagctgtcc gtgtcgccgc aggacggtgg cagcgatgag ccgcgccagg ccgcgatcat
    10981 tcccggcaag ctcgcggtac tcctgacgag ccatgccata ggcgagcgcc tcgtcgaacg
    11041 tccggcctct gtcgagcaca cctgcaatca gctcgactgc aagctctcgc gccagtagcc
    11101 cggggggaca ctggcgcggt tcagacgtct ggcgttcctt cgttcgacgc tggccggctg
    11161 ccatgcccgg tcagccccag gggccgcggg ctgggccact tgctgacctg aggctcgccc
    11221 cccgccccat ctcagcggcc aacctctgca gctcggcgat cctgttctcc gtgttggggt
    11281 gggtcgagaa caggttgtcc atgccttggc ctgagagcgg gttgatgatg aacaggtgcg
    11341 ccgtcgtcgg tgcctcctcg gcgacagggt tcatgatccg gcgggcataa tggtcgatct
    11401 tctgcagcgc cgacgccagc caaagcggct cgccgcaaat gtgggcaccg atgcgatcgg
    11461 cctcgtactc ccgcgaccgg ctgatcgcca tctgcaccag catggccgcc agtggcgcga
    11521 gaatgatcgc cgccagcgcg ccgatgatgc cgagcggccc gccaccctcg ccgtcgcgcc
    11581 ggccgccgaa cagcatgctg aactgcatca tgttggcgaa catggtgatc gcgcccgcca
    11641 cggtggcggc caccgtcatg atgagggtgt cgcggttcct gatgtgcgcc agctcgtgtg
    11701 caatgacgcc agccacctcc tcccgatcca gcatatttag caggccggtc gacgcgcaca
    11761 cagccgcccg cgacggactg cggcccgtgg cgaaggcgtt cggctggtcg ctgtgcatga
    11821 tgtagacccg aggcatcggc aggccggcgc ggtctgcgag gtcgcggacg agggcgtaga
    11881 actccggcgc cgtccgctgg tcgacctcct cggccccgta catccgaagg accatgcggt
    11941 cggagttcca gaggctgaag aggttcatgc cgagcgcgat gatgaacgca atgacgaggc
    12001 ccgtcttgcc gccgacaagg cccccgagcg aaaccaggat ggccgtcatc agcgccagca
    12061 gaacgcctgt cttgatataa ttgctcgtca tcaacatctc cctgacggac acgcccgccg
    12121 gccgcgcccg tatgccgggc tcggcgcggc acaactacat ggtatatagg aaggcgctcg
    12181 ttcaagatcc gcaccactgg caggacgcca tgaccgacca gaccgaaccg acgcccccga
    12241 agcggcggct cactccggcc gccgagcgcg cgctcaagga agcggaggag cgccgtcgcc
    12301 aggccggctc tgaccagcgc ccgcaggaga tcggtggccg caagggtcct gaccccgtcc
    12361 ggtacggcga ctgggagaag ggcggcatcg cctcggattt ctgagctgca accggcccga
    12421 gatggagcgc ggggcgccgc gtgatctgcc ttgaaccggc aagcaatggc cgctatagca
    12481 acgcggccgc gcgcacgcgg aactcatgtc ctgcaaaggg ttttgattgc aggccgaacg
    12541 cgcgccctgg agaggtggct gagtggttga aagcaccgca ctcgaaattc gtttgcagcc
    12601 atcccacaag gttccgacct gtcccatcac gtcacgtgtt ttcaaaggga taggtcgcca
    12661 cgttcaacat ccagtgccat attatcccga cgcgtgctgc cgagttcggt agcatatggt
    12721 agtagcggcc cagcttacgc gtggacggcg gccgtagcgt ggggccgcat tgccggctct
    12781 tgagcagccc gacagcgacg gtcatggccg gcgggcttcg acggggcaag cgccaacggc
    12841 cgtcctgggg tctgagagcg gcgcgttgaa gcgcgcggcc ggtcctgaga accgcccgcg
    12901 gcagcaaaaa accgcgttgc cgagcgctgg gcggccgtga gccgccgggc agctgcagta
    12961 agccggcgga ggccagcttt ggccggtcgg accttggaag cgaccttcgc caataccttg
    13021 gcggatgttc gcgatcgtgc gagacgcaac aaagcattcc gacttaccgc aaagtcttgc
    13081 gccaagtaga aaggggttgg aaaagtcagg caaaatcttg gcagaatttt tcacacgtcc
    13141 gccaagctag actaagggcc acgcatttcc gtcgacaagt cgccagccaa ctacttaacc
    13201 ctgcaattct tcgagtgatg tcaaaacgcg cgcaggtggt aagtccagat attcgttggg
    13261 cgcggcgaag cgattgatcc acactgttct gaaacccgcc cgcgtcgcac cagcaatgtc
    13321 ccagcgattc gaggactgga acgaaatcgc gtcaggtggc agccgaaaag cgccggtcac
    13381 gatcccatag gtggtggagt cagtcttgaa gcgcttgacc tgatcaaccg aaaaaacctc
    13441 gtccagcacg gactccagac cggcggaacg cacggcggca tcaagcatgt ccggcgaacc
    13501 atttgaaagg atggccagcc ttgcaccggc tttcttgagc gactcgagta ccgaaggaac
    13561 ctccgggtag cagtccagct gctggtaagc atggagcaag tccgcgcgta gcgcccggtt
    13621 cgcagaaggc acgcaatcga aggcgtagtc cagcgcttgc tccgtcagtt gccagaaatc
    13681 agcgtacgcc cccatgagtg tccggaccca ggagtactcc agctgcttgg tgcgccagat
    13741 ctcggaaagc ttctgcgcat ccggcccgat ggcctgagcg tgccttctta ccgcggaatg
    13801 gacgtcgaac agcgtgccat aagcgtcgaa aacgtacgcg gcgtattgca tgtaagctcc
    13861 acttcggcga gagcgcagat tggccgcatt gctaagccaa cctgtcattg ctcggtggtc
    13921 agactatcgt ttgccaactg ccttccgcaa gctcactacc gctccgcggc gtctcgcacc
    13981 gatagtaggc agcctgcgct tgtatctggc gcgccgcatt gacaggcgca cgatcgcagg
    14041 ctgagtcgcc gcgtccttaa cgcgtcactg atcgatcgca ctcctgtaac tgagcttttg
    14101 aaatgggtcc attgaaatgc gtcagctggc agcaacgtac gcgagaccgg atttcgacgc
    14161 cggagggatc aggaaggctg taccgatgtg gcaaccttct cgaagcttgg tctggccgct
    14221 agctatagcc cacctttgct gccggacgac ccgtcagccg agttcagtca ggataaccgg
    14281 catcgccttc gtgtggatcg gcgccaccac ggcgttggac tcgcaggtga gaaagccaat
    14341 atctcgaagt ctcgctcacc gtcgcgcacc agcttgacgc cgcgccaggg cgtccacagg
    14401 ccggtagcgg taggcataaa tcgtctgacc cgatccggcc tgcggccaca ttgccgtttt
    14461 ggacatgcta accctgcgcg atcggctcgg tccggatcac ggcgccctgc gcgccgacgt
    14521 ggtaccgaag ctcaggtgca cgaagtgcgg cggccggcaa gtcggcatca tctcgactcc
    14581 cggaacgaag gagtacggcg gcaatcctta tctgaaacga aggatgagcg gtgccacgat
    14641 ggcggcgcgg cttagacgcg gcgcttgcgc cggaaggttg tcacctgagc accggcctgc
    14701 ctacggagct tcgaggcttg gccctcaaag ctgcccggcg ccttggctgt tccgcgccag
    14761 aaatgcgatc gattccctca gtcgcggcaa ctcagcctcc aatcgcgcgt ccgtcaagag
    14821 cagtgcaacc ttctacattg gtactgcgat gcgcgattcc tactccgaat ctcagaattt
    14881 tgaagcgctc cggtgctgct ccgacatttc gcataagcgc gccagcagca tcgtttcggc
    14941 cacccatttt tctggtacgc ggctggccgt ggtggcccgc aaccgccgcc cacagtgagt
    15001 tgaatgtcgt tgcgctccag ccctcggtcg ccggcctcaa cagctcgcgg agaccaagca
    15061 gctcgcggac ggcatagcca tcctgatcat gccggctcat tggcggagat ggccgccagt
    15121 caccaagctg cggaccagcc gtttcggccc acaaatccgg acatttcgcc tgaaagtcgc
    15181 gcgaaaactt gccaacgtcg tgcagagcca cgggacagac gagaaagcgc tgcgcgtttt
    15241 cgtcagacgt gccgagcagc gcggccaaag tggccggcgc cgcgggttta gatcgagcag
    15301 cgcctcggcg acggcggcga catcgaggct gtgatgagca acggggtgcc acgccagctg
    15361 gccactcgag gctccaccag atttgtccca gaagctgaga taagccgcca cctctgagga
    15421 ccctccaaaa atgcgtcgac ttctattgtc caactcgcca cagatcgagt gctggtcttc
    15481 cgtacccatc aagccaccat ccgaggcctc ggtctcaatc gccatgcctc tttggcggcc
    15541 ccagcggttc aatcgtcctc tgccgcttgc aaacaccaga tcctcgcgtg aagtcttcaa
    15601 tgggcttgcc ggcgccatct ttccgcttcg cttcggcctc ttcgaggacc caagcccaac
    15661 cgtcagcggt gaggtttggc atgagatcat caatgccttg cgtcgaaatg ggacccttgg
    15721 tggggcggac tggacgcgat ttgtcagcca tgctcattgc ctccaggttc ttcaaatggc
    15781 ctcgctggct caatgcgtgg tgtcgtgttt acatcaatgg cttcagtgaa gtgcaactcg
    15841 cacaccctcg cccgtcgcgc cacagcatca tgacgcatat gacgccgctg ctcctgatcg
    15901 ccgacggctg aactgcccag tatggggcgc gcaagccaca ttgcgtacca attattccgg
    15961 gcagcgcgat caatgattgc cacctttgcg cctgcttcag cgagtgccag ggcagatacc
    16021 tgatccaggc ccgccgcgcc gccggtgaca acggctatcc gtccatcgag tttcaatcgt
    16081 tcgagtaccg tcttttcagg ccctttttgt tcggcgagcg ttgtcgggtg atcgacaaca
    16141 ccttcgcatt ggacaacaag gcaacaatga ccggttatgc gttgagcctc acgtgatatc
    16201 agcgcagcaa accgattcat tccataagcg gatcaatccg ccaggcctga cctcgtgttc
    16261 ctgcttcctt catgaaatcg gcgaaggcgc gaacggccgg ggcaagctca cgtcggctcg
    16321 gatagaccag acttaccgga agagacggca tggaccagtc gggaaaaagg cggatcaatc
    16381 gcccctccgc aatgtccgga ccgcaaatat aggctgaaac cacgccgatc ccggctccgt
    16441 tgcgtatcag ccgatagatc gtcagcacct cgtccagatg cacgcgcggt cgaggtatga
    16501 tgcgccgtgt ctcgtcgtct tttcggaact cccaggcaag ggtgcggcca tcagctccca
    16561 acatttcgat cgtgtcatga tggtcgagat cgcctatgga aaccggagtg ccacgcctct
    16621 cgagataggc aggcgatgcg cagagatagc ggtgcatggt accaagctgg gtggatacca
    16681 gcgtggagtc cggcagggcg ccgaacctga ttgcgcaatc gacgccttcg gtgaccatgt
    16741 ccgccagacg cgtcgacagg tcgagcgtta gccgaatctt gggatagcga tcaagaaaca
    16801 ccggcaacag ctcgctaaga atgttcacgc caaagccaat cccggcgctg atcttgatat
    16861 gaccgcgcgg ctcgccggca aagctgctca catattcaac ggcttcatcg atcccctgca
    16921 gcatgggagc gcatctttcg agcagcgccg caccggcttc cgtgaggacg acgcttcttg
    16981 tggtgcgctg aaagagccgg gttcccaacg cagcctcaag aagcgccacg gcccggctga
    17041 cattggactt gggcagcccg agcgcgcgtg cggcggccga aaacccaccg agagcggcaa
    17101 cgcgttcgaa tagccgcaac tgattgagat ccagcgcctc tattgtccct atcatggaac
    17161 ggtgtttcca atcggagtgt cttctgtgcc ttctgatgta cggtcatatt cggcctatca
    17221 caaccggata ccggcggcat tgtgcgttct gtcggcacaa tgaggggtca tggtcatgaa
    17281 tgcccgtgcc ggaggaaaga ggaacgcaat gactgatcta ttcacacctt tcgagctgca
    17341 tggcaccaca ctgtcgaatc gtgccgtcat ggctccgatg acccgcaccc gcgccaccga
    17401 agacggcgta cctaccgcgc tgatgcgcga ctattacgct cagcgtacag atgcagggct
    17461 gatcatcacc gagtgcacgc agatctcgga tcaggcccac gggatcatcc gggcgcccgg
    17521 gatccatcgt gacgaacaga tcgatggctg gcggatgatc acaaagagcg tccacgacaa
    17581 gggcagccgg atttttaacc agatctggca ccccggccgg gtctcccatc ctgagatcct
    17641 gggcggagac gccccggtcg cgccctcggc aatcccggct gagggggatt tcttccttcc
    17701 ctcggggcgg gtcccgttca ccgtgccgcg cgcgctgcgg ctggaggaaa tccccgctat
    17761 cgtggccgac ttcgcccggg ccacgcgcaa tgcgcgtgac gccggttttg acgggatcga
    17821 aattcacggg gccaacggct atctgctgca gcagtttctg gaggacggtt ccaacacccg
    17881 gtccgatgcc tatggcggaa gcatcgagaa ccgcgcacgg ttcacgctcg aagtggtcga
    17941 cgccgcaatc gcgcagtggg atgccgaaca cgtaggaatc cgcctgtccc cggccggggt
    18001 ccattacggg atgcgcgaca gcgaccgtcc cgcgctttac gactatctga tccgcgagct
    18061 cgccacgcgg tccatcgcct acattcacgt cacgaggccg aacaaggcct catatgacgc
    18121 cgggccggtg gccattgaag acgttgccgg cttcaccagg gccaggttct caggcccgat
    18181 catcgtcaat ggcgggtatg accgggagaa ggccgagcgc gtcctcgtca ggggtgatgc
    18241 cgatctcgtg gctttcggcg tccccttcct caccaccccg gatcttctca ctcgcatgaa
    18301 ggtcggagca gaactgaacc gtcccgatcc agcgaccttc tatggggaag gacctgtcgg
    18361 ctacaccgac tacccgtttc tgacaccggc atgaagtctg aaatgacatt caggccggcc
    18421 aggaaagcgg tcagcgagcg atccgaagag gtcgcccgcg ctggccccag tcgcgccggc
    18481 aacgcacaag ccacggcccc tgttcgcctt cgcaggcagc tggcaccggt ggcaaggacc
    18541 gatccggaaa gacgggccgc ccgtggacat cgagctctac ggtttcctca cgacctttcc
    18601 gaacgccctg acgcagaccg tagcggtcgc cggtcatcct gactgaggat gagcagttcg
    18661 agcaatggct gtgcggaaca ccggcggagg ctttcagcct ggtccagccc ttcgatgccg
    18721 agcgcatgcg gatcgtgcag gagggcttcg agaagcggta tctcctcgcg gcttgatcgc
    18781 ccttccgtag gtcccggcca atcggggcgt ctcagccctg acatagctcg ctcaagcgtc
    18841 aaaccacggg acgggcggcc gggcgatcat cagccagaag atggccagta cggccgcgaa
    18901 cgccggaaag ccgaaggcaa accatatctg gaacagacga tggtaggccg gtggcagagc
    18961 gcgtccctgc tgcttggcgg cgatggccag atcccgcatg cgcatctgca tccagaccac
    19021 cggaagccaa aaggccccgg ttacgatata gaggacgatg gacaaagcga tccacccctc
    19081 ccacagggaa tagccggcca gatgagcgag ccaaacgccg gtcatgggct gggcgataac
    19141 cgccgtcgcg gtgaacagga agtccgcgat cacgacgatc cgggccacgc cggcgacgac
    19201 ggcgggatca ccggtccgat gcgcgagcag catgaagaac gcgatgcctg ccccggtgcc
    19261 gagcaggacg gtcgctccga ggacgtgcag gtacttcacg aggaggtaaa gcatcagcgg
    19321 tcttcgagga tggccaaggc gaccaggttc agcgcgagaa tgggccacac cttcagcatc
    19381 ggcccaagcg ggtcggccca caggctcggc agcaccagcg tgccggcaac gaggtagaac
    19441 acggtgacca gcagcgcggc gtagagcgcg atccgggcgg tgcggcggat ggcaactccg
    19501 atgccgatca ccatgtccgc cagtcctccg gcgatgaccg aaaggcctgc ccattttgtg
    19561 atgccgcccg cattcaggta agcgacaccg gtggcgtaac ccggccccac ggagatgagg
    19621 ccggtggcca accagaacag ggcgaaaatg ccgatcacca gcggcttgag caggtacagc
    19681 ctggcgaacc agcgctgctg caccgaggcc ggctcggcca gcaggctcgc ttccagcgtt
    19741 cgtgggatga tgttcgtcaa gtgcatccag ggcgacggat cgccgaccgc gccccggctg
    19801 atctcacggc gggccgtgct gcggatcggc ggccgccaac ccagccacgc gacaagatcg
    19861 ccggcccgat aggctaccgc cgcggcccac gatggcagtt gcaccacata cggcgctctc
    19921 catcccaacc agcggcgata gaggagaacg atttcggtga acgacagctt ctgcggcccg
    19981 gcgatctcga gggccagccg ggtcggaaca tcatcccgca gaaagtaaag aacggtccgc
    20041 gttacgtcat cgagctgaac gacctgcagc gggccggtct ccggcattgc gggcaggatc
    20101 ggcaaggcgg cgagcccgcg gaacagcgcg ctcgccccat agaccggtcc gccgacgatc
    20161 accgacggcc tgaggatcac ccagtcgagg tccatggcca tcagcttctc atcgccggcc
    20221 agtttgctgg ccgaaaacgc cgtcaaggct ccacgatcga caccgatcgc ggagaagtgg
    20281 atgatcctgc gcaccccttt gcgcaggcag gcctcgaaca gcgcggccgg gcctttcacg
    20341 tgcacggccg cggtcgaatc ccgggggctg tcctgcagaa ctccggcgca gttcaccacg
    20401 gcgtcgacac cgtcgagcag tggcaaccag ctttcgacgt cctgtcgggc cagatcgact
    20461 ctggcccatt gcaccgcgcc gagaccggcc ggcgggggct ggccgtgggc gatggccacg
    20521 agctcatgcc ctttggatga gagtgcggcc aggacggccg atccgaccag tccggtcgcg
    20581 cctgtgagca acaccctcaa ggttctagcc tcgattcaga cgacgcaacg aacagaaaat
    20641 tccaggcacg taagctgcaa ccacgcccca acgcagattg ttcccggcat cagcggccca
    20701 attgcgcgga atactcttcg catcctcctg tcttggctta tagaagaacg ggccaattgg
    20761 ggggcgagtg atgcctgaag tggtgggcgc attccgacaa ttacttccct tcaccctact
    20821 cagccgttca tccggccgat cagtgattgc gcgcgtcgga cctcaatcgg acttcccttg
    20881 tcaggacttt ggcctcccga gaatatccgc gagcctctcc tgcttcttct cggtccgcgt
    20941 cagatccagg ccggacagca ggtccgcgat atgtcttttc atcagcgccg acgcctctgc
    21001 tgcgttgccg tcagctatcg cgtcgacaag gtcacggtga gcgtcgcact tgcaggtcgt
    21061 atcacggtgg cgccaataaa gggcaatgat cagcgaggaa cgggaaacaa gatcccggat
    21121 gatctcagtg aaaatcgaat ggtcagcgat ttcggccagc gtaatatgaa aatgcgccga
    21181 aagcctgatc gcctcgctgc tttgatctaa tctgatcgcc tcgcgctctc tttccaaatg
    21241 ctggcgcaag tgccggatgt gctcgggcct cgctgccttc gcagccaacg cagcaagcct
    21301 cggctcgatc agcttgcgcg cgtcgaaaac ctcgcgtgcc tccttgatcg aaggctgcgc
    21361 gacgaaagct ccgcgattgg gctccagccg caccacgcga tcatgggcaa gcgcctgcaa
    21421 tgcggaccgg accaccgttc ggctcaccga gtagatggag gccagctcgt cctcgggtag
    21481 cttggttccc ggcgcgaggc ggcggctcgt aatggcctcc cgcagggaat cgtagacagg
    21541 ctgccagcgg gcagccacct tcgcagtctt tcttatgtta ttggacttat cgagcataag
    21601 actaggattc ccgcagcttc agcggcggct gttttctgga atgaggctct tgcttcaatt
    21661 ctgaggtcgc ttgcctctca accaggagaa aagcggctga aatgtccact gaagcgtttc
    21721 gcacgcggag gcgcatagcc gccgatcctc gacgtcctca atcccaaatc aaccaaattc
    21781 tcggcgagct tgaccgcaca tgctacgcca tcgatgaccg gcaggccgtg atcggccgat
    21841 agtcgttcgg ccagatccgt catgcctgcg caaccgagca cgatggcttc ggcccgatcc
    21901 tcggctacgg cgcggccgat ctcggttgag attttcgact gggcgtccga gcctgcgact
    21961 tcaaggtcga gaaccgggac atcagccgca cgcacccgtg cacagcggct gaccaggcca
    22021 tacctgacga gattgtgctc gatcgcggga attgagcggg ccagggtcgt gatcacgctg
    22081 aacttcccgg ccaccaggct tgcaacgtgg aatgccgcct cgccgatccc gatgaccggt
    22141 gccggggtga ggcaacgcgc tgcgtccagg ccggtatcgt cgaaacaggc gatgacatag
    22201 gcgtccatat cgggagcctt ctgcatttcg ccaatgaggc ctggaatggc gaacacctcg
    22261 tcgaaatagc cttcgatgct cggcgggccg tccgcgggat tgacggcagt gatctcggtt
    22321 cccgaagccg ccgcggaccg cgccgccgct ccgattttag cggtcatgga gggagtggta
    22381 ttcgggttga tgaccagaat gcgcatgatc tagagctcgg ctccgagata gagcttccga
    22441 acgcgttcat cgctcaaaag gtcggcggag gcgcccgaga gggccaccct accggtggat
    22501 agtgcatagg cgcgctgcga gatgcgcagc gccatgcggg agttctgctc gacgaggagc
    22561 acgctgacct tgtcgtccct gttgatcgcg acgatggagc gggcgatgtc ctgcacgagc
    22621 ttcggcgcga tccccagcga gggctcatcg agcagaagca atctcggctt cgacatcagc
    22681 gcccggccga tgacgagcat ctgctgctcg ccaccgctca tcgtgcccgc tgcctgtgag
    22741 tagcgctcct tcagccgagg aaagcgcgtc aggaccaatt cgagcgatcg gccaatctcg
    22801 gctttgtcgg tgcgcgtgaa ggcgcccatc agcaggttgt cgcggaccga catgtaggga
    22861 aaaacccgcc ggccctccgg caccatggag atgccgagct taacaatttc atcagagggc
    22921 aggccgtcaa tgcgcttgcc ctggtaatgg atttctccgc tcctgatccg ccgcagcccg
    22981 gtgatcgcac ggaggatgga ggacttccca gccccattgg cgccaatgag cgccaccgtc
    23041 tccccttcac tgacgctgag ggatacaccc ttcaaggcgt atacgtggtc gtagtagagc
    23101 tcgacatcgt tgagggtgag caggtcggtc atggctagat cccgagcgcc tcgtcttccg
    23161 cgccgagata ggcctcgatc acggtcgggt tctcctggat ctcctgcggc gtgccctcgg
    23221 cgatcttctg gccgaaattg aggacgacga tccggtccga gatgttcatc accgcaggca
    23281 tgtcgtgctc caccagcagc acggtgacgc ctcgctcgcg cacgccacgg acgatctcga
    23341 tggcacgcct ggtctcttca tggttcatgc cggcgaacgg ctcatcgagc agcaggacct
    23401 gcgggttcgt ggcgagcccg atcgcgatgc cgagagcccg gaggtgtccg tgcggcaggt
    23461 tgctcgccag ctcgtgccga tagcccccga ggccgaggaa gtcgatgatc tcgtccgtcg
    23521 agcgtgcgaa ctccgcctcg tcctgctgcg ccgccctggt gccgaagaag aagcccgcaa
    23581 gattggcgcg cgaccggagg tggtgggcga tgatgacgtt gtcgcgcacg gtcatcgtct
    23641 tgaagatcgt ggtctcctgg aacgtgcgca cgacgcccat tcgggcgacc ttgtgcgggg
    23701 cgaggccgga tatccgctgc ccccggaagc gcacctcccc ggccgtcggt gaaatgaagg
    23761 aggcgatcag cttgaaaagg gtcgatttgc cggctccgtt cggaccgatg accgacagga
    23821 tctcccgctc cttcacagtg aaggagatat cattgttcgc aacaagaccg ccgaagcgtt
    23881 ttgtcaaacc agatatttcg aggatatccg gcatggacta gctctgcttc tgcctggcta
    23941 agcggatgct caagaggcca ttcggcagca cgagcatcag aatgatcagg atggacgagt
    24001 agatcaaaag ctggaaaacg cccgtctgga acaggaggtc ccagccgaag taaaggacga
    24061 acgtgccgag catcggcccc aggacgtagc cgagcccgcc aagaaagcaa ttgagcatga
    24121 agttgatgga atcggcgacg ccgaagctcg agggatacat ggattgcgtg atcgcggcga
    24181 acatggctcc tccgatgccg ccgaggaacg aggatatggc gaacgccagg atgcgaatgt
    24241 agctgatgtt gacgccgatt gaggatgcga gctcctcgtt ctgctggagg gactggcaga
    24301 tcctgccgat ccgcgaattg acgaggcggt agagggcgag gtagcagagc gccatcagca
    24361 ggaccgacgt gacgtagaat gccagcctcg gattggcaag cgtcgcaaaa tccgggatta
    24421 aagtgatgcc gaagatcgtc agcccgctgg ggaggggaat gttcacgatc cctttggcgc
    24481 cgttggtaat gggaacggcg agcgcagtga gccgcgccac ctcggacaat gtcaacgtga
    24541 ccattgcgaa atagacgccg cgcagcctca gaatcggcag gccgatcagg acgctggccg
    24601 cggcgcagag caggcctgcg atcggcagcg tgagccagaa cgaagctccg tattgcgtga
    24661 cgaggatcgc ggatacatag ccgcccatca gcgcaaaagc gccttgtccg atattgatgc
    24721 ggccgatgta gaacgtcagc catacgccgg cgctgatgat gctgagcagc gcgacggagg
    24781 tgagcgtata ataaagatcg ctgcgacccg tggccgagat cagcagtggc acaccgacca
    24841 ggagaacggc caggaaggcc aaagccgagg cgatcttctg ctgcgtggtc atccgatcag
    24901 ccccacggct ttcccatcag cccgttgggc cggatgctga ggaagatcat gagcaggacg
    24961 aagatgacga gataggtgac gtcgccgaag ttgcgcaaca gcgtcaagcc gaccgactcc
    25021 atcatgccga ggatgaagcc tccggcgatc gcaccaccga ccacgcctgc accgcctatc
    25081 atgaccatca gaaaggcttt gatcgaaatc ggaccgccga tgccggaatt gacgccggtg
    25141 atgctgacga gaagcccgcc gacgagcccc gccaacatgg ccccgagagc aaacccgatc
    25201 atcgaatagc ggtcgacgtt gacgcccatg agctgtgcag cgacccggtc ctgggcgaga
    25261 gcgcgcatgg cgcggcccgg ccggctgtac tgcatgtaga gcacgaacgc agcgatcatc
    25321 gccgccgcca ccacgccgac ggcgatgcgg tcatagggca tgatgatgat atcggagacg
    25381 aatacgccgt tgatgatctt cggcacgccc ctctgcttct cgccgaacaa aagcaagatg
    25441 atcgcgtcga gaaaaaacgc aatcgcagcc gcgagcagca tcgtgctttc ctcgcgggcg
    25501 cttcgcttga tgacgggccc gaacaggaac ttctcgatga tcgcgccgac tgcggcgagc
    25561 gttacgcccg agcagagcac cgcaactgga aagggaagcc caagctggcc atagaccgta
    25621 taggtcacga atccgccgag cacgtacatc tgcccgtggg caaaattcag tacgttcatc
    25681 agcgcaaaaa tcaacgtcag gccaagcgcg atcagagcat actgggctcc gagatagagg
    25741 ccgttcgcga tgacctgttc catgtcgact gtccgtccat tgtggggtgg gcccggagcc
    25801 gccgcactcc gggcctcgaa catgaaccag attgtcagtc tacttcgccg acgaacagcg
    25861 tttcgaactt cccgtcgcgg aactcattga cgaccatcgg cacggaaagc tggcgcttct
    25921 gaccgaacga ggtcatgccg acatacttca atgtgccgtc tttcacgaag gggttcggcg
    25981 ccgaaaaggt ctccatcgtc ttcttgaatt cctcgacgtc gtcgatcgcc ttcggattcg
    26041 ccttcagcgt ttcgatgatg tattcgagcg cgtagacctt cgtattcgac tcgtcgttat
    26101 attccccgta tttcttggta tagcgctcga tgaactcttc catctccgcc gtccggattt
    26161 cgggagtcga tgcgccgccg accgagatga agccgttcgc gagatcgcct gcgccctcct
    26221 gcagcacggt ggcgtcgtgc gccgtttcgg cagacatcag accttcatac ccgagctcgc
    26281 gcgccgcgcg gatcagcaaa ggcgcgttgc ccggcgagac acccgaaagg accagcaggt
    26341 cgggcttcaa ccgcacgacg ggggtcagga caggcgtgaa gtcccgcgtg tcggtctggt
    26401 aggtgtcgtt ctgcgcgacg acctcaagcc caagctgttt ggcagcggca acgcccgcat
    26461 cccgctgact cagcggatcg gactcgttgg cggcaacgaa cgcaaccgtt ttgatgccct
    26521 tgttctcctt cagatacttg taaatggctg gcgcggattg gtagctcgcg accatgccga
    26581 gcacggcatt ggaggccgga ggcgcataga gctccttggg aaaggcgtag gggaaataaa
    26641 caattccttt tgactctgcg accggacgga cggcggctgc cccgtcgtca acgttcgggc
    26701 cgaccacgta gcgaacgccc tcctgcgcca tcttctccat cccggcgatg gcacgcctcg
    26761 gatccttctg atcgtcgaag gtcacgaccg tgatcttgta ggtttcgccg ccgatctcga
    26821 caccgccttt ttcgttcagc cagtcggcgc gggtctgcat ggagcgcacg ttcgagatgc
    26881 cccaggcggc cgccggacca ctcgtgacgc cgacgaaacc gatt
//
LOCUS       DASVDU010000311         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1446783, whole genome shotgun sequence.
ACCESSION   DASVDU010000311 DASVDU010000000
VERSION     DASVDU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446783"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..679
                     /locus_tag="VF226_20910"
     CDS             485..679
                     /locus_tag="VF226_20910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076506.1"
                     /translation="MPDFEQADIFTTNATVLPRSFWKYLLPFQRVIRIINDRGAHHDL
                     TRALFAREFEAVALKAAASA"
     gene            complement(708..1619)
                     /locus_tag="VF226_20915"
     CDS             complement(708..1619)
                     /locus_tag="VF226_20915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076507.1"
                     /translation="MPDGTAHSIRTTESANHRARHAWRISAVWRSSILRAVFGLVLLA
                     SGIAMVGHGILWNIPVFQGEYVRPPSVPVRDLLSVERRPGEGDLAFVERATQTVHRAF
                     GYCPGQVSTRYMWLYTGAMSIARITPKSILTSLSLPRSLRQVPFNFGLISADLIGFFD
                     CGLCHQHDALLREVLAKAGIHSDLLTLRNHVVLRVSIDGEFWYADPLYGVGPIQVDFR
                     DAGAARATLVEAYRDNPLLVGIERLEPDVMADRYLAIESHRLWGADLLAFMRVAQKVL
                     LIAQKIIELAVAVIGSVLLVGAFRKPG"
     gene            complement(1866..2645)
                     /locus_tag="VF226_20920"
     CDS             complement(1866..2645)
                     /locus_tag="VF226_20920"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7076508.1"
                     /translation="MALWYGNAAKNRIIGEIVARANQSPEGQSPLVFDYGAGTGGSWP
                     DILAKHPNIRLVCYEPHRKSAAILANRLRGRATVYEGNIADLNIQADYIVSFSVLEHV
                     ADRPAYMANAARLLAPHGRFYLNYDDGHFRAPIDLLNPRTWKRGITHALIRLAAPIRY
                     RLGDEGQYLGRVTQQEADDLATRAGFRIVSERYENLEIFKALSKLIPPNRRDEFAVFW
                     ASVEDTLNARFDDPAEERKGDRSALWSIMASRTLELVRATR"
     gene            3100..>3969
                     /locus_tag="VF226_20925"
     CDS             3100..>3969
                     /locus_tag="VF226_20925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7076509.1"
                     /translation="MHVVQLGYDDSVFRIGAPSDTLKRQLDYGRVLAALRPGAQMTFL
                     AFSRNRGARAFQRENVRFVPVPGGRVAQPIALIRALAAIHRRQPIDVVSSQSPFEDGW
                     ATLAFGKANRIPTVAQIHFDIFSPFAARGGSFPLLSRARTALGIRSLRHFTAIRCVGN
                     GIKRAILDRGLHHAVSVIPVPVTMTPKERDANPSRVLYVGRLDSEKRMRDWLHVAAAV
                     LERRPETAFDIVGNGADRADLEHLARSLGIADRVRFHGFVPYERLPDFYRRAAVFLLT
                     SSYEGFGRVLVEAQ"
BASE COUNT          796 a         1269 c         1162 g          742 t
ORIGIN      
        1 tccagccgcg tgcgcgtctc cgtcgaaaag gccgacgccc accagaccga tgcggcgatc
       61 tgcatgagca ccgtcgcgat cagggcgatc gggacacggc ggacgatgtc gtgcggttcc
      121 ttggggatgt ccgtcatgcc aaccttcccc ccgcacggcg cggcacaaac aagaaagccc
      181 cctggcgggg gcggtaggac ttcgtcattc gagactcctt ctctcttcgt cgaacacgaa
      241 tcgacagcat cagggcggcc acgaccgggc gtgacggcgc cggtcggggc gcactcttaa
      301 aagacacgca gctgcgagcg tcagaatgct gggacaggtc ggttggtgaa cacggcgtcg
      361 tgtgatcgac agccagcgcc catagcggcg atcaagtctc gaatggctct ggcccttggt
      421 tccatcctta cgtcagaagg acgagcctgt gcgcactcac agccgagcga tggcgcgaga
      481 tgcgttgccg gatttcgagc aggccgacat cttcacgacc aacgccaccg tcctaccacg
      541 cagcttttgg aagtatctgc tgccgtttca acgggtgatc aggatcatca acgaccgtgg
      601 cgcacatcac gacctcactc gcgcactgtt tgcgcgcgag ttcgaggctg ttgccctgaa
      661 ggccgcagca tctgcctgat gaaaggcctc ggaaaacgga gatgggatca tcccggcttg
      721 cggaatgctc cgaccagaag aacgctgccg atgaccgcga ctgccaactc gatgatcttc
      781 tgggcgatca gcaggacctt ctgtgccacc ctcatgaaag ccagcaggtc cgcgccccaa
      841 agcctatgcg actcgatggc aagatatcgg tccgccatga cgtccggctc aaggcgctcg
      901 atgccgacca gcagcggatt gtcgcgatat gcctcgacca acgtcgccct cgccgcccca
      961 gcatcgcgga aatcgacctg tatcggcccc acgccataga gcgggtctgc gtaccagaat
     1021 tcgccgtcga tcgaaacgcg cagaacaacg tgattgcgaa gggtgagaag atcgctgtga
     1081 attcccgctt tggccagcac ctcccgcagc agcgcatcat gctgatggca caagccacaa
     1141 tcgaaaaagc cgatgaggtc cgcggaaatc agcccgaagt taaacggaac ctgacgcaaa
     1201 gagcggggta aagacaggga agtcagaatg ctcttcgggg tgattcgggc gatcgacatc
     1261 gcgcccgtgt aaagccacat atagcgcgtg ctcacctgac cggggcagta gccgaacgca
     1321 cgatgcaccg tctgagttgc ccgctccacg aatgccagat cgccctcacc cggcctccgc
     1381 tcgacggaca gcagatctcg cactggcaca gaaggagggc ggacatactc gccctggaac
     1441 acggggatgt tccacaggat gccgtgaccg accatcgcga taccggaagc gagcagcacc
     1501 aagccgaaga cggcgcgtag aatcgacgag cgccaaactg ctgaaatccg ccaggcgtga
     1561 cgggcgcggt ggtttgccga ctcggtagtg cgaatgctat gcgcggtgcc gtcgggcatg
     1621 tccatgtcat tggaaaaccg acagagttgc ccccgaaaac tatcaggggt tgtcttacca
     1681 tgcaacaacc ctagataaat tgcgggatgg cgtccctctc cttagattgc tagacggcaa
     1741 ccaagacaaa gctacgaggc gcgatgtcct caaccacagg catcaaacgc gcaacgcttc
     1801 gggatcaggt gcggacctgt gcccccctac atgaggcaag cttagtcaga gattgcttgc
     1861 cccctctacc gggttgccct gaccagttcc agcgtgcgag acgccatgat cgaccaaagc
     1921 gcggaccggt cgcccttcct ttcttccgcc gggtcgtcga atctcgcgtt cagcgtgtct
     1981 tcgaccgatg cccaaaagac cgcaaactcg tcacgccgat tgggcggaat cagcttcgac
     2041 agcgctttga atatctcaag gttttcgtat cgctcagaga cgatgcgaaa cccggcccgc
     2101 gtcgcgagat cgtcggcttc ctgctgggtc acgcggccaa gatactgccc ttcatctccg
     2161 agcctgtatc ggatcggagc ggcaagacgg atgagtgcgt gcgtaattcc tcgcttccac
     2221 gtgcgtgggt tcagcaggtc tatcggtgcg cgaaagtgcc cgtcgtcgta gttgagatag
     2281 aaccgcccgt gtggcgccaa gagccgcgcc gcatttgcca tgtaggccgg ccgatccgcg
     2341 acgtgctcga gaaccgaaaa actgacgatg taatcggcct ggatgttcag gtccgcaata
     2401 ttgccttcgt aaaccgtcgc ccgtccgcgc agccggtttg ccaaaatcgc ggcggatttc
     2461 cggtgtggct cgtaacaaac cagccgtatg ttcggatgtt tcgccagaat gtccggccaa
     2521 ctcccgcccg tgccggcgcc gtagtcgaat acgagcggtg actgaccctc aggcgactga
     2581 ttcgcacgag cgacaatctc gccaatgatc cgattctttg ccgcgtttcc gtaccagagc
     2641 gccatgtgcg gcttatattc gacgtagcac ctttcatgga aggccctaag gcacgtggga
     2701 cgaaagctca cgttgtaatg gaatttcgga gtgccgcgcg ccgtctgacg ttggagccgg
     2761 gaaaatcaca atctgcgaga ctccagttcc ggacaggcct ttcccgacac ccccatttga
     2821 ttacctggga tggcggctac cgccaggatc ggcgaggcac ttcccaaacg tccagttccc
     2881 ttcctgatcg cccacacaga cgctgtcacg ggctcagttc agtctggctg aggcggacat
     2941 caagcattcg accatttgtt aatgccatta ccattaggac cacagcggct aagagtcgtg
     3001 acgctatgat ctcaccccac tatcaaggtg gggctatcgg aagtaccctt gacctcggtt
     3061 ccacagggga ttgggacgct ctcgtgttgt gggattgcga tgcacgtcgt tcagctcgga
     3121 tacgatgaca gcgtgtttcg gatcggtgcg ccgtccgaca cgctcaagag gcagctcgac
     3181 tacggccgcg tgctcgcggc gctgcgtccg ggcgcgcaaa tgacgttcct cgcgttctcg
     3241 cggaaccggg gcgccagggc atttcagcgc gagaacgtcc gcttcgtccc tgtgcccggc
     3301 ggccgggtcg cgcagccgat tgcgttgatc cgcgcgcttg ccgcaatcca ccgccgtcag
     3361 ccgatcgacg tcgtatcgtc ccaatccccg ttcgaggacg gatgggcgac gctcgccttc
     3421 ggcaaggcga atcgcatacc gaccgtcgcc cagatccact tcgacatctt ctccccgttc
     3481 gccgcgcgcg gcggaagctt cccgcttctg agccgcgcca gaaccgcgct cggcatccgg
     3541 tcgctcaggc acttcaccgc gatccgctgc gtcggcaatg gcatcaagcg cgcgatcctg
     3601 gatcgaggcc tgcaccatgc cgtcagcgtg atcccggtcc ccgtgaccat gacaccgaag
     3661 gaacgggacg cgaacccctc gcgtgttctt tacgtcggaa gactggactc agagaagcgg
     3721 atgcgcgact ggctgcacgt ggccgccgcc gtcctcgagc ggcggccgga aaccgcgttc
     3781 gacatcgtgg gcaacggcgc cgaccgtgcc gatctggagc atcttgcccg ctcgctcggc
     3841 atcgccgacc gcgtccggtt tcacggcttc gtaccctacg aacgtttacc cgatttctac
     3901 cgccgcgccg ccgtatttct cttgacctcc agttatgagg gtttcggccg cgttctcgtt
     3961 gaagcgcaa
//
LOCUS       DASVDU010000312         7247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1671244, whole genome shotgun sequence.
ACCESSION   DASVDU010000312 DASVDU010000000
VERSION     DASVDU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671244"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF226_20930"
     CDS             <1..616
                     /locus_tag="VF226_20930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7076510.1"
                     /translation="PKGISTVVVEKGTPGLSFGKKEKKLGWNSQPTAMVIFEDCRVPV
                     ANRIGAEGDGFKIAMKGLDGGRINIAACSLGAARACLEAARAHLDVRMAFGRKLAEFQ
                     ALQFKLADMATELEAARLMVHRAAGALDAGDPNATLYCAMAKRFATDVGFNVCNEALQ
                     LFGGYGYLKDYPVERYLRDTRVHQILEGTNEIMRVIIARRLLGL"
     gene            complement(658..1404)
                     /locus_tag="VF226_20935"
     CDS             complement(658..1404)
                     /locus_tag="VF226_20935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076511.1"
                     /translation="MNKIDLAGDVAIVTGGAQGIGRAIVERFAASDATVEIWDLDGKL
                     AEEAARSIGGAVHARAVDVADAAAVAQAAKSAEATHGPIDILVTSAGIAGPNAKTWEY
                     PLDDWARIMRINVDGTFHCCRAVVPGMIAQGYGRIVTIASIAGKEGNPNAAAYSSSKA
                     AVIALTKSLGKELAGYDIAVNTITPAAARTRIFDQMSEEHINFMLSKIPRGRFVEPRE
                     IAAMVAWLASAENSFTTGAVFDLSGGRATY"
     gene            complement(1422..2216)
                     /locus_tag="VF226_20940"
     CDS             complement(1422..2216)
                     /locus_tag="VF226_20940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7076512.1"
                     /translation="MNSASDKARGAIVVGGGTGLGLASAKALAEAGWRVFLTGRRADV
                     IDRASASMPPGVSSALAGDATREADVERVVAAAQAFLGDIHALVVSSGATAVGSVVDA
                     AYDDVVHVLHTNLLPLFLFGKHTVRNMPAGGAIVAIASVAGTVPHPERLAYCASKAGL
                     IGMVRQMALDLAPRRIRVNAVSPSLVLTDLARSAIAQSPDPEATLANRLSRHPLGRLG
                     TVEEVGAAVAYLCSDEAAWITGQNLILDGGLSLAAMQATLHDKSFR"
     gene            complement(2200..3384)
                     /locus_tag="VF226_20945"
     CDS             complement(2200..3384)
                     /locus_tag="VF226_20945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX7076513.1"
                     /translation="MSIITEVDVIPFTFKVENLALGAHRAMGVSNLRFEKGGTLEVMR
                     YAVRIATKDGLEGHYVTHWVGTSAALGQTVMLAPHLLGRNAEHREAIYDDLKRELRAY
                     DHMGHGPLDIALWDLVGKKYGMSVATMLGAYKERLPTYASTHHGQDEPGGLDSVEAFA
                     EFARACREKGFPGFKIHGWHDGNKHREAKVVLKVREAVGDAMEVMVDPGCELRTWADA
                     LYVGRACDEAECLWYEDPYRDASVSVTGQRLLREKLKTPLLVSEHIRGPEQKAAFALA
                     GGCDMIHTDPEYDMGITGAMKIAHMAESLGMDVQVHACGPAHRACVAAIRNTHFYELA
                     LVGPNMPNLVPPVYLCGYSDELSAVGPDGCVPTPTGPGLGVTYDWKFIRKQATAVHEF
                     RL"
     gene            complement(3416..4690)
                     /locus_tag="VF226_20950"
     CDS             complement(3416..4690)
                     /locus_tag="VF226_20950"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX7076514.1"
                     /translation="MILTLTILLCLFLALGMPIAFSMGLAAAGALALDGTVPLIVVPQ
                     KFYSSLDSFPLLAIPLFILAGAVMNVGGITEILIRLSQALVGHVRGGLGQVTVFTSLL
                     FATISGSAAAGAAAVGGMLIPAMERDGYRREDAAVITAAAAILGPIVPPSILMVIYGS
                     MTGLSIGTLFLAGVGPGLLITVILMITVYRMAGRMNVRLYPRSSLPQIASAFRQSLPA
                     LVMPAIIVGGIVSGVFTATEAGAIAVAYGIAYALVTRRAGLKTFMANVTRAAVASSSI
                     MVILGGAALFGWIIARAGVPAMLAAYLQGLTDSSAVVMFLILVVLLVVGIFIEPIPAL
                     IMLVPVLQPIAQAYGFDPYHFATVVTYAVLLGSLSPPVAVLVLITCKIANIDYTRTTR
                     PLLPVFAMLVSALFIIAFVPAITLAVPRLTGS"
     gene            complement(4687..5160)
                     /locus_tag="VF226_20955"
     CDS             complement(4687..5160)
                     /locus_tag="VF226_20955"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7076515.1"
                     /translation="MLVRLVGHLIGMAKAAMVAAFVAMIALTLVQVVNRYAIGFTIFW
                     TEELIVLLLVWSMLLGLPVQLWMREEIVVDVIPLRNETLARGKALLAAGCSIAFCGLL
                     LVTGYDFARRGWPVGSPALGLSRFWFFVPIPISAALSILALVARARGPAREAFDS"
     gene            complement(5186..6250)
                     /locus_tag="VF226_20960"
     CDS             complement(5186..6250)
                     /locus_tag="VF226_20960"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX7076516.1"
                     /translation="MPDRACQPAMQGGTSMSESGRFTINRRALGKLAIGAAVSAALPG
                     SAAIAQAGARTIRLAHHVTTQSEQHAAAENFAKKVAEYSGGSLRVQILPAAQMGGQRE
                     IIESVSLGTLEMGYGESGLYANYLPSFGIIALPYLYRDFDHWQKVVDGEIGQSLARDL
                     EQRANIKIVNWMTAGYRNTFLRTKPVTKPSDFRGVKIRLPEAPVFVRTFATLGAQPTP
                     IPAPEMYTALQTGVVDAMEGSAEVGYTFKIFEVTKFLSKTRHILLDGSFAINSGFFNS
                     LNKTQQDAIMRAAQEAAATQRTEHFERDRQWLDKLAAAGTLQINEVDLPAFAQALAGL
                     QNEFAANARATDLLEKIRKL"
     gene            6343..7074
                     /locus_tag="VF226_20965"
     CDS             6343..7074
                     /locus_tag="VF226_20965"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7076517.1"
                     /translation="MSSLANGLRLLALLKSPHVTLRVSEAARELRLPKSTVSRLLKEL
                     TQAGLLERDGEGPGFRSGPELFRLGSLYRARLPVENRIDEALNALVHRYPATAYVGVL
                     RGVDLIVLRRHEGVHPVRFIQEPGSCLPAYATAVGKALLARRPFNEIVEALPERLVCE
                     TRDVNMTRDALLDELETARRRGYAELDDRLLGIGAVGVAIGLAPGRDIGFAICFSRDI
                     APSDKEAIAQELCELAREIGRLCDD"
BASE COUNT         1265 a         2320 c         2444 g         1218 t
ORIGIN      
        1 acccaagggc atctccacgg tcgtggtgga gaagggcacg cccggcctgt ccttcggcaa
       61 gaaggagaag aagctcggtt ggaacagcca gccgacggcg atggtgattt tcgaggactg
      121 ccgcgtgccg gtcgcgaacc gcatcggcgc cgagggcgat ggattcaaga tcgcgatgaa
      181 gggtcttgac ggcgggcgca tcaacatcgc ggcgtgctcg ttgggcgcgg cgcgggcctg
      241 tctcgaggcg gcgcgggcgc atctcgatgt ccgcatggcg ttcggccgca agctcgccga
      301 gtttcaggcg ctccagttca agctcgccga catggcgacg gagctcgagg ccgcgcgcct
      361 gatggtgcac cgtgccgccg gcgcgctcga cgccggcgac cccaacgcga cgctttactg
      421 cgccatggcc aagcgtttcg cgaccgatgt cggcttcaac gtgtgcaacg aggcgctcca
      481 gctcttcggc ggctacggct atctcaagga ctatcccgtg gagcgttacc tgcgggacac
      541 acgcgtccac cagatcctcg aaggcaccaa cgagatcatg cgcgtgatca tcgcgcggcg
      601 cctgctcggc ctctaggcct cctgatccgt gagagccggc ctgtcggcgg gcggtggcta
      661 gtacgtcgcc cggccgcccg agaggtcgaa gaccgcaccg gtcgtgaagg agttctcggc
      721 cgaggcaagc caggcgacca tggcggcgat ctcccgcggc tcgacgaagc gtccgcgcgg
      781 gatcttggag agcatgaagt tgatgtgctc ttcgctcatc tggtcaaaga tgcgggtgcg
      841 tgcagcggcg ggcgtgatgg tgttgaccgc aatatcgtag cccgccagtt cctttccgag
      901 cgacttcgtc agtgcgatca cggcggcctt cgagctcgaa taggccgccg cgttcgggtt
      961 gccctccttg ccggcgatcg aggcgatggt gacgatgcgg ccgtagcctt gcgcgatcat
     1021 gccgggaacg acggcccggc agcaatggaa cgtgccgtcg acgttgatgc gcatgatgcg
     1081 cgcccagtcg tcgagtggat attcccacgt ctttgcgttc gggccggcga tgccggcgct
     1141 ggtaaccagg atgtcgatcg ggccgtgcgt cgcctcggcg ctcttggcgg cttgcgccac
     1201 ggcagcggcg tcggcgacat cgaccgcgcg ggcatggacg gcgccgccga tgctgcgcgc
     1261 cgcttcctcg gccagctttc cgtcgagatc ccagatctcg acggtcgcat cgctcgccgc
     1321 gaaacgctcg acgatggccc gtccgattcc ctgtgcaccg cccgtcacga tggcgacgtc
     1381 gccggccaga tcgatcttgt tcatgctggt ttcacccacg ttcatcggaa actcttgtcg
     1441 tgcaaggttg cctgcatcgc ggccagggac agtcccccgt cgaggatcag attctgcccc
     1501 gtgatccatg cggcttcgtc cgagcacaga taggcgacgg cggcgcctac ctcctcgacc
     1561 gtgccgaggc ggccgagcgg atgccggctc aggcgattcg cgagcgtcgc ttcgggatcg
     1621 ggcgactgcg cgatggcgct gcgggcgaga tccgtcagca cgaggcttgg cgagacagcg
     1681 ttcacccgga tgcggcgggg cgcgagatcg agcgccatct gccggaccat cccgatcaat
     1741 ccggccttcg acgcgcaata tgccagccgc tcggggtgcg gcacggttcc cgcgacggaa
     1801 gcgatcgcga cgatggcacc ccccgccggc atgttcctta cggtgtgctt gccgaaaagg
     1861 aacaacggga gcaggttcgt gtgaaggacg tgcacgacgt cgtcgtaggc cgcatcgacg
     1921 acggatccga ccgccgtcgc ccccgaactg acgacgagcg cgtgaatgtc gccgagaaac
     1981 gcctgcgcgg cagcgacgac ccgctcgaca tccgcctctc gggtcgcgtc gccggcaagc
     2041 gcgctgctca cgcccggcgg catcgaggca gaggcccggt cgatgacatc cgcccggcgg
     2101 ccggtcagga agacgcgcca gccggcctcg gcaagggcct ttgcggaggc gagcccgagc
     2161 cctgtgccgc cgccgacgac aatggcgccc cgcgccttgt cagaggcgga attcatgaac
     2221 cgccgtcgct tgcttgcgga tgaacttcca gtcgtaggtg acgccgagtc ccggcccggt
     2281 gggtgtcggg acacatccgt ccggtccaac ggcactcagc tcgtcggagt atccgcagag
     2341 atagaccggc gggacgaggt tcggcatgtt cggcccgacc agggcaagct cgtagaaatg
     2401 cgtgttgcgg atggccgcca cgcaggcccg atgcgccgga ccgcaggcat ggacctggac
     2461 atccattccg agcgactccg ccatatgggc gatcttcatg gcgcccgtga tgcccatgtc
     2521 gtactccggg tcagtgtgga tcatgtcgca accgccggcg agcgcgaagg ccgctttctg
     2581 ctcgggaccg cggatatgct cgctgacgag aaggggcgtc ttcagcttct cgcgcagtag
     2641 ccgctgtccc gtgaccgaga cgctggcatc ccggtagggg tcttcgtacc agaggcattc
     2701 ggcctcgtcg caggcgcgtc cgacgtagag ggcatccgcc caggtcctca gctcgcatcc
     2761 cggatcgacc atgacctcca tcgcgtcgcc caccgcctcg cggaccttga ggacgacctt
     2821 cgcctcgcgg tgcttgttgc cgtcgtgcca tccatggatc ttgaatccgg ggaagccttt
     2881 ttcgcggcag gcacgcgcga actcggcgaa ggcctcaacg ctgtccaggc cgcccggctc
     2941 atcttgtccg tgatgcgtgc tggcataggt cggaagcctc tccttgtagg cgccgagcat
     3001 ggtcgcgacg gacatgccgt acttcttgcc cacgaggtcc cacagcgcga tgtcgagcgg
     3061 gccgtgcccc atgtgatcgt aggcccggag ctcccgcttc agatcgtcat aaatggcctc
     3121 gcgatgttcg gcgtttcgtc ccaggagatg cggggccagc atgaccgtct gtcccaacgc
     3181 tgcgctcgtg ccgacccagt gggtcacgta atggccttcg agaccgtcct tcgtcgcgat
     3241 gcggacggca tagcgcatca cctccagcgt cccgcctttc tcgaaacgca gattgctcac
     3301 acccatggcc cgatgcgcac cgagtgcgag gttttccacc ttgaaggtga aggggatgac
     3361 gtcgacttcg gtgatgatgc tcatgatgcg gcgaactcct gtctgtgtgt ctggctcagg
     3421 aaccggtgag gcggggcacg gcgagcgtga tggcggggac gaacgcgatg atgaaaaggg
     3481 cggagacgag catcgcgaac acgggcagca gcggccgcgt cgtgcgcgtg tagtcgatat
     3541 tggcgatctt gcaggtgatg agcacgagca ccgccaccgg cgggctcagc gagcccagaa
     3601 gcacggcgta ggtcacgacg gtggcgaagt ggtaagggtc gaagccgtag gcctgggcga
     3661 tcggctgaag gaccggaacc agcatgatca gggccggaat cggttcgatg aagatgccga
     3721 ccacgagcag cacgaccaga atcaggaaca tcaccacggc gctgctgtcg gtgagtccct
     3781 gcagataggc cgcgagcatg gcgggaacgc cggcgcgggc aatgatccag ccgaacaacg
     3841 cagccccacc caggatgacc atgatgctcg atgacgcgac cgcggcgcgc gtcacgttcg
     3901 ccatgaatgt cttgagcccg gcgcgccggg tcaccagggc gtaggcgatg ccgtaggcga
     3961 cggcgatggc gcccgcctcg gtagcggtga acacgccgga tacgatgccg ccgacgatga
     4021 tggcgggcat gacgagggcc ggcagcgact ggcgaaacgc actcgcgatc tgaggcaggg
     4081 acgagcgggg atagagccgc acgttcatcc gtcccgccat cctgtagacg gtgatcatca
     4141 ggatcacggt gatcagcagg ccgggcccca cgcccgcgag aaaaagcgtg ccgatcgaaa
     4201 gtcccgtcat cgagccgtag atgaccatca ggatgctcgg cggaacgatc gggcccagga
     4261 tggccgcggc tgccgtgatg acggcggcgt cctcccgtcg gtacccgtcg cgctccatag
     4321 ccggaatgag catgcccccg accgcggccg cacccgccgc cgccgacccc gagatcgtcg
     4381 cgaacagcag gctcgtgaag accgtgactt ggcccaggcc gccccgaaca tgtccgacca
     4441 gcgcctggga cagcctgatc aatatttcgg tgatcccgcc cacattcatg acggcgccag
     4501 ctaggatgaa tagtgggatc gcgagcagcg gaaacgagtc gagcgaggag tagaacttct
     4561 gcgggacgac gatgagggga accgtgccgt ccagcgcaag ggcaccggcc gcggcgagcc
     4621 ccatcgagaa ggcgatgggc atcccgagcg caaggaacag gcacagaagg atcgtcagag
     4681 tcagaatcac gaatcgaacg cctcacgggc cggcccgcgc gcccgcgcga cgagcgccag
     4741 gatcgaaaga gcggcggaga tcgggatcgg cacgaagaac cagaagcgtg aaaggccgag
     4801 ggcgggcgac ccgaccggcc agccgcgtcg cgcgaagtcg tatcccgtca ccaggagcag
     4861 gccgcagaat gcgatcgagc accccgccgc aaggagcgcc ttgccccggg cgagcgtttc
     4921 gttccgcagg gggatgacat cgacgacgat ctcctcgcgc atccagagct ggaccggcag
     4981 gccgagcagc atggaccaca ccaggaggag cacgatgagc tcctccgtcc agaagatcgt
     5041 gaagccgatg gcgtaacggt tgacgacctg tacgagcgtc agggcgatca tggcgacgaa
     5101 ggccgccacc atggcggcct tcgccatccc gatgaggtgc ccgacgaggc ggacgagcat
     5161 cgtgtcggcg tcgccggcgc gtccgtcaga gtttacggat cttttcgaga agatccgtgg
     5221 cccgcgcgtt cgccgcgaac tcgttctgca gtcccgcgag ggcctgggcg aaggccggca
     5281 ggtcgacctc gttgatctgc aaggttccgg cggccgcgag tttgtcgagc cactgcctgt
     5341 cgcgttcgaa gtgttcggtc ctctgggttg cggccgcctc ttgcgcggcc cgcatgatcg
     5401 cgtcctgctg cgtcttgttc aggctgttga agaagcctga attgatggcg aagctgccgt
     5461 cgagcaggat gtgccgcgtc ttggagagga acttcgtcac ctcgaagatc ttgaacgtgt
     5521 acccgacctc ggcggacccc tccatcgcat cgacgacgcc agtctgaagg gccgtataca
     5581 tttcgggcgc agggatcggc gtgggctgcg cgcccagcgt ggcgaaggtg cgcacgaaca
     5641 ccggcgcttc cggcaggcga attttcacgc cgcggaagtc ggacggcttg gtcaccggct
     5701 tcgtgcgcag gaaggtgttg cgatagccgg ccgtcatcca gttgacgatc ttgatgttcg
     5761 cccgctgttc gagatcgcgt gccagggatt ggccgatttc gccgtcgacg accttctgcc
     5821 agtggtcgaa atcccgatag agatagggaa gggcgatgat gccgaacgac ggcaggtaat
     5881 tcgcatagag gccggattcg ccgtaaccca tttcgagcgt gccgagcgac actgactcaa
     5941 tgatctcccg ctgaccgccc atttgggcgg cgggcaggat ttggacccgc agcgagccgc
     6001 ccgagtattc cgcgaccttc ttggcgaaat tctcggccgc cgcgtgctgc tcggactggg
     6061 tggtcacgtg gtgggccagc cggatggttc gggcgcccgc ctgggctatc gccgcgctgc
     6121 cgggcaaagc cgcggagacc gcggcgccga tggcaagttt ccccaacgcg cgcctgttga
     6181 tcgtgaaacg accgctctcg ctcatgctcg ttcctccttg catcgccggt tggcaggccc
     6241 gatccggcat cgatcccgat tttggcgact acgctaggtg agcttgacac cgaaatcaac
     6301 ttttgtccca aatggacaaa cgttattgct gtatatggaa caatgagttc cctcgcgaac
     6361 ggcctccggc tgcttgccct gctcaagtcg ccgcacgtca cgctccgcgt cagcgaagcc
     6421 gcacgggagc ttcggctgcc gaagagcacc gtctcgcgcc ttctgaagga gctcacccag
     6481 gctggcctac tcgagcgcga tggcgagggg ccgggtttcc gctcggggcc ggaacttttc
     6541 aggctgggca gcctctaccg ggcccgtctg ccggtcgaga accgcatcga cgaggcgctg
     6601 aatgcgctcg ttcatcgcta tccagcgacg gcatacgtgg gcgtgctgcg cggcgtcgat
     6661 ctcatcgtgc ttcgtcgcca cgaaggggtt catccggttc gcttcataca ggaaccggga
     6721 tcgtgcctgc cggcctacgc gaccgcggtc gggaaggcat tgctggcgcg gcgccctttc
     6781 aacgagatcg tggaggctct gcccgaacgg ctcgtctgcg agacgcgcga cgtgaatatg
     6841 acccgggacg ctttgctcga cgagctcgag accgcgcgcc ggcgcggata cgccgagctc
     6901 gacgaccgtc ttctcggcat cggcgccgtc ggcgtcgcca tcggtctcgc gccggggcgc
     6961 gatatcggat ttgccatctg cttcagccgg gacattgcac cgtccgataa ggaagcgatc
     7021 gcgcaagagc tgtgcgagct cgcgcgcgag atcggccgcc tttgcgacga ttaattttgg
     7081 ttgacggact gatcggaagc gtcacgccgg tcgcggcggg accgggatgt ccgtaacggc
     7141 ggtccggcgc cgaatcatct ttgatgtgcc ggacgatttt gatacgtcac agggccggcc
     7201 gagtgcctgg accaagctct ggcagaacga aacgatgaac gacacga
//
LOCUS       DASVDU010000313         3956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_479153, whole genome shotgun sequence.
ACCESSION   DASVDU010000313 DASVDU010000000
VERSION     DASVDU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479153"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1110)
                     /locus_tag="VF226_20970"
     CDS             complement(37..1110)
                     /locus_tag="VF226_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX7076518.1"
                     /translation="MTVQYPRFKAAACHIASVFLDSARSVDKAVSWIGEAAGQGASLV
                     AFPESYVPGFPIWAALRAPIVNHDYFRALAREALSVDGPEIARIRTAARRHGVHVSLG
                     FTEGTTASVGCLWNSNVLIGPDGSILNLHRKLVPTFYEKLVWANGDARGLRVVQTDIG
                     RLGVLICGENTNPLSRFTLMAEGEQVHISTYPPFWPTRPPSEAGAYDLRRAIEIRAGA
                     HAFEAKVFNIVSSGFADQALKDALAGEDPQVRDVVENSPRSVSMILDSSGNLIGQALS
                     EDEGILYAEIDTALCVEPKQFHDVVGYYNRFDIFKLKVDRSKRDPATFTEQPMEVTEA
                     ANGHLEPDEGRQGKPAPSGALPV"
     gene            complement(1127..1921)
                     /locus_tag="VF226_20975"
     CDS             complement(1127..1921)
                     /locus_tag="VF226_20975"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076519.1"
                     /translation="MELIRGLMALGLTETESRFYLAALELGQASVREIAEKAGLTRTG
                     AYDIYARLLHQKLVAEVANGSGRAILVTAEPPQQLRSMFEQRRQRLSDLLPELESLHN
                     RSRSKPRIRFYQGLEGIKAVMEETLTCRSKKLLGILSMRDLYQVPGRAWMDDLVRRRI
                     EAGVSLRVIRSPSNDVHPHWSESRRDLRELRYAPAGFACSMTTYIYDEKVAFISSQRE
                     HFAMTIESADFVAMQTQLFEVLWQACTVPEPPPSARNRRGRYQMTQ"
     gene            2153..3013
                     /locus_tag="VF226_20980"
     CDS             2153..3013
                     /locus_tag="VF226_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076520.1"
                     /translation="MAEVVFALAINGLVWGLIIALIALGLSIIFGLLDVINIAHGDFF
                     MLGTVGGLVLSLWTGSFWIALLLVPLLGFALGLVFERLVIRPTIRQAALTIVTTFGLS
                     MMIQESVRALYGPQPRRMQAPIDGTIPLFGIDYDIYRVVAAVFAAVCLAGFFLFLKHT
                     KIGTWIRAVRHDPETATALGVPVVRICAITFGLGTAIAFLGGAIAAPITTVEFRTGVD
                     ILPFCFMAVIIGGLGNLEGTVAAAVMLAVLEGVVTSVTDPTIARIFSLAFMSLVLLLR
                     PHGLFTGAKR"
     gene            3010..>3956
                     /locus_tag="VF226_20985"
     CDS             3010..>3956
                     /locus_tag="VF226_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076521.1"
                     /translation="MTRKLNTVALGLLAAGLISVPFVLPAFGASFWVNIIAEILIWSL
                     LASSVNLLLGYVGLLSFGQALFFGFGMYGATLSVLHLGAGFWTGMAAGVLAATLMALI
                     AGVLAVRLTWHYFAIITIVFSLIFYFAAMSAKPLTGGDDGLSFTAPTVFDFGGTTLTL
                     ADPTAQYLIILAIVAGCYVLKGLLVNSPLGLAFAAVRENDRRAALIGINVYRVRLTAF
                     VLAGALAGISGALFAFYGRYASASYMFYHVSGEAVVWAIVGGTGSLLGPLFGTGALIA
                     FRELVSTAWENYLLAVGAITILVVMFAPRGLAGIWNDLVT"
BASE COUNT          686 a         1205 c         1186 g          879 t
ORIGIN      
        1 caccccaacc cttgagtatg cgaggcgtaa ctcaatctaa accggaaggg ccccagacgg
       61 ggcaggctta ccctgtcgtc cttcgtccgg ttccaggtgg ccgttggccg cttcagtcac
      121 ctccatcggc tgctccgtga acgtcgcggg atcacgcttc gaccggtcga ctttcagctt
      181 gaagatgtcg aagcgattgt aatagccgac cacatcgtgg aactgcttgg gttccacaca
      241 gagtgccgtg tcgatctccg cgtaaagaat accttcatcc tccgacaggg cctgcccgat
      301 gagattcccg gacgaatcga gaatcatcga gacactcctt ggcgagttct ctacgacatc
      361 gcgaacctgc ggatcttcgc ccgccagagc gtccttgagc gcctgatccg cgaatccaga
      421 ggagacgatg ttgaagacct tggcctcgaa cgcgtgcgct ccggcccgaa tctcgatcgc
      481 gcggcgcaga tcgtaggcgc cggcctccga cggcggacgc gtcggccaga acggcggata
      541 ggtcgagatg tgcacctgct cgccctcggc catgagcgtg aatcgggaca gcggattcgt
      601 attctcgccg cagatcagca cgccgaggcg gccgatgtcg gtctgcacga cgcgcaaccc
      661 gcgagcatct ccgttcgccc agacgagctt ctcatagaag gtcggcacga gtttgcgatg
      721 caaattgagg atcgagccat cgggaccgat cagcacgttg gagttccaca ggcatccgac
      781 gctggcggtc gtgccctcgg tgaacccgag tgaaacatgg acgccgtgcc gccgggcggc
      841 ggtccggatg cgggcgatct ctgggccgtc gacactcagc gcctcgcgtg ccagggcgcg
      901 aaagtaatca tggttgacga taggcgcccg cagagccgcc caaatcggga aacccggcac
      961 gtagctttcg ggaaatgcca ccaaactggc cccctgaccg gcagcttcac cgatccagga
     1021 cactgccttg tcaacggagc gggcactgtc gaggaaaacg gacgcgatat ggcaggcggc
     1081 agccttgaac cggggatact gaaccgtcat cggaacatcc tcaacgtcat tgtgtcattt
     1141 gataccggcc ccgtcggttc cgcgcggagg gcggcggctc ggggactgtg cacgcctgcc
     1201 agagcacctc gaacagctgc gtctgcatgg caacgaagtc cgcgctctcg atcgtcattg
     1261 cgaagtgctc tcgctgggac gagatgaagg cgaccttctc gtcgtagatg taggtcgtca
     1321 tgctgcatgc gaatcccgca ggcgcatatc tgagctcccg cagatcgcgg cggctttccg
     1381 accagtgcgg gtggacatcg ttgctcggag atctgatcac ccggagcgac acgcccgctt
     1441 cgatccggcg ccggaccaga tcgtccatcc acgcccgccc cggcacctga tagagatccc
     1501 gcatcgacaa gatgcccagg agctttttgc tgcggcaggt cagcgtctct tccatcacgg
     1561 ctttgatgcc ttcgaggccc tgatagaagc ggatgcgtgg cttggaccgc gagcggttgt
     1621 gaagcgattc aagctccggt agcagatcgc tcagccgctg gcggcgctgc tcgaacatcg
     1681 aacgaagttg ctgtggcggc tctgcggtca ccaggatggc gcggccgcta ccgttggcga
     1741 cctcggcgac gagcttctga tggagaagcc gcgcatagat gtcataagca ccggtgcggg
     1801 tcagtcccgc cttctccgcg atctcgcgta cagaggcctg accgagctcg agcgccgcaa
     1861 gatagaaccg actctccgtc tcagtcagcc ccagcgccat cagccctcgt atcaactcca
     1921 ttggtccgcc tgctttgaga ttcggccgag gccggttggc ttgcacgata gctgcgatgt
     1981 cggacgtcgt ctacaatccg tccgtatcga cgcgatcacg gcgccccgaa tgcacataat
     2041 tgcagcactt tccgaatgga tgcgcctgtc gagtattacc gacttgacac atctgtccgt
     2101 cgctagtcag acttatggtc tgtgacacat ggtccagggt cgggggagca gattggccga
     2161 agtcgttttt gcattggcca tcaatggtct tgtatggggc ctgatcatcg cgctcatagc
     2221 gctcgggctt tcgatcatct ttggcctctt ggatgtcatc aatatcgccc acggcgattt
     2281 ctttatgctg ggcaccgtgg gcggtctggt gctctcgctt tggactggat cattctggat
     2341 cgcactgctg ctggtgccgc tgctcggatt tgcgctgggc ttggtgttcg aaagactggt
     2401 cattcgacca accatccggc aggccgccct gacgatcgtt acgacgttcg ggctttccat
     2461 gatgatccag gagtcggtgc gtgccctcta cgggccgcaa ccgcgccgca tgcaggcgcc
     2521 gattgatggc acgatcccct tgtttggcat cgactatgac atttatcgcg ttgtcgccgc
     2581 agttttcgcg gcagtctgcc tggcgggatt cttcctgttc ctgaaacata cgaaaatcgg
     2641 cacctggatc agggcagtcc gacatgaccc cgagaccgct acggcccttg gggttccggt
     2701 tgtccggatt tgcgcgatca ccttcgggtt gggcacggcc atcgcatttc tgggcggggc
     2761 aattgccgcg ccaatcacga ccgtcgaatt ccgtaccggc gtcgacatcc tgccattctg
     2821 cttcatggcg gtgatcatcg gggggctggg caatctcgag ggtacggtgg cggcggccgt
     2881 gatgctcgcg gttctggaag gcgtcgtgac gagcgtgacg gatccgacca ttgccaggat
     2941 cttctcgctg gccttcatga gcctcgttct tcttcttcgc ccacacggat tgtttacagg
     3001 agcgaagcgg tgaccagaaa actcaacacc gttgcgctgg ggctcctggc cgccggactc
     3061 atatcggtcc cattcgttct acccgcattc ggtgcgagct tctgggtgaa catcattgcg
     3121 gagatcctga tttggtcgct cctggcttcg agcgtcaatc tcctgctcgg ctacgttggc
     3181 ctcctctcgt ttggacaggc tctctttttt ggtttcggca tgtacggagc gacgctctcc
     3241 gttcttcatc tgggtgcagg cttctggacc gggatggctg ccggtgtgct cgcggcaacc
     3301 ctgatggccc tgattgcggg cgtattggca gtccgcctga catggcacta tttcgccatc
     3361 atcactattg ttttttctct catcttttat tttgcggcga tgagcgcgaa gccgctgacc
     3421 ggaggcgatg acggcctgtc attcactgct ccgactgtgt tcgatttcgg cgggacaact
     3481 ctgaccctcg ctgacccgac ggcccagtat ttgatcattc tggcgatcgt cgccggatgc
     3541 tatgttctga agggactgct cgtaaacagt ccgctcggac tggcattcgc ggccgtgcgt
     3601 gagaatgacc gccgtgctgc gctcatcggc ataaatgtct atcgggtgcg tttgacggca
     3661 ttcgttctgg ccggagccct tgccggaatt tcgggagcac tgttcgcctt ctatgggcga
     3721 tacgcctctg cttcgtatat gttttatcat gtctcaggag aagcagtggt ttgggcaatc
     3781 gtcgggggaa ccggctcact cctcggcccg ctgttcggca ccggagcact gatcgccttc
     3841 cgcgagctgg tctcgacggc gtgggagaat tacctgctcg ctgtcggcgc catcacaatt
     3901 ctggtcgtca tgttcgctcc ccgtggcctt gcaggcatct ggaatgacct cgtcac
//
LOCUS       DASVDU010000314        17254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1060320, whole genome shotgun sequence.
ACCESSION   DASVDU010000314 DASVDU010000000
VERSION     DASVDU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060320"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VF226_20990"
     CDS             complement(<1..1445)
                     /locus_tag="VF226_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7076522.1"
                     /translation="MAQVLTEAAPHETAAAGSSQGNSACESTTLVVENMHCGGCMRKV
                     EKALAEFPGVISARANLSARRVTVTSSKGRVTTDDLVDVLGKAGYKAAPLLEDAASDK
                     SADKEFLRRVGVAGFAAMNIMLLSVAVWSGQAGDMEQSVQTLFHWISALIALPAVAYA
                     GQPFFRSAMQALRSRRLNMDVPISLAVTLATAMSLFQTIRGSEHVYFDAAVSLLFFLL
                     IGRFLDQSMRTRAAGAAANLLGLRGSTATVLFPDGSTERLSARALTPGMRILVATGER
                     IGADGKIVEGRGAIDESLITGEAEPVTAEPGRQVFAGTLNMGAPLVIEATATNESTLL
                     AEIGRLMTAAEQGRGRYVRLADRAASYYAPAVHSLGLMTFVGWLIMGHGWEPALTAAI
                     SVLIITCPCALALAVPAVQVAATSRLFGRGVIVKAADGLERLAETDTIVFDKTGTLTL
                     GEPRLIEPQSCSDDLLIRAAALAAASRHPYAR"
     gene            complement(1430..1951)
                     /locus_tag="VF226_20995"
     CDS             complement(1430..1951)
                     /locus_tag="VF226_20995"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX7076523.1"
                     /translation="MRTKGAEMSARQVRQGLNGRHVLIAVLAFFGTVFLANGIFIYAA
                     LSTYTGVVADEPYRKGLRYNDRIAAAERQEQLGWTAETSLDPAAGLTLVLRKADGGPV
                     SGLRVSGILGRPSTVEYDRQLVLQEVEPGRYVAGVEALSPGAWLVDLKAIWPGTGETE
                     PVYRLRKRLWLKS"
     gene            complement(1954..3471)
                     /gene="ccoG"
                     /locus_tag="VF226_21000"
     CDS             complement(1954..3471)
                     /gene="ccoG"
                     /locus_tag="VF226_21000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX7076524.1"
                     /translation="MTSMPQDLKPADFISEAAPGVEKLDVAAINRKDRRQLYATRQKI
                     YPRLARGRFRTVKWAVMAITLSIYYALPWLRWDRGPNLPDQAVLLDFANGRLFLFFLE
                     IWPQELYYITGILVISALSLFLVTAAAGRVWCGYTCPQTVWTDLMLAVERFWQGDRNA
                     RIRLDKEPWSATKIFKKVMTHLSWLAIGLATGGALVFYFRDAPTLAAELVTGTAPAIA
                     YLFLGIFTFTTYLLGGIAREQVCIYMCPWPRIQGAMVDRHTLLVSYRPERGEPRGPLR
                     KGQGWEGRGDCIDCKACVAVCPMGIDIRDGAQFECIQCALCIDACNEIMDKIGRPRGL
                     ISYDTVAKQEAKAKGVVHEPLKLVRPRTILYSGLIALVAVVMALGLVSRTTLEVNVLH
                     DRGVLFVPLSDGGVRNGYTVKVLNKQHVPVDVVLSIDGLQGAAMRIVGHDAGDGALSI
                     PADNLREVRVLVTVPPEHLGQLTPPSTRFDFVVKDVATGTEMRRDAIFQSASGRS"
     gene            complement(3883..4749)
                     /gene="ccoP"
                     /locus_tag="VF226_21005"
     CDS             complement(3883..4749)
                     /gene="ccoP"
                     /locus_tag="VF226_21005"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX7076525.1"
                     /translation="MSRKEVDEVTGIETTGHEWDGIKELNKPLPKWWVWTFYATIIWS
                     IGYWILYPAWPTMADYTKGVLGYSQRQAVANQVAEARAAQGKFREALASTPLERVKDD
                     SELLRFAMAGGAAAFATNCAPCHGRGAQGFVGYPNLNDDDWLWGGSIEEIHNTIRFGI
                     RSGHADGHDSQMPRFGIDGLLERKQINDVAEYVLSLTGRATDQDAVARGSEIFAEQCV
                     SCHGEDAKGNREFGAPNLTDAIWLYGNSKEAVVESIRTGRGGQMPVWEGRLDPVTIKS
                     LAVYVHSLGGGQ"
     gene            complement(4746..4925)
                     /locus_tag="VF226_21010"
     CDS             complement(4746..4925)
                     /locus_tag="VF226_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX7076526.1"
                     /translation="MTYETVASFSQVSSLLLFIGIFLGVVVYACWPGNRNRFEIAQKQ
                     ALDLDKDHNSRGGCK"
     gene            complement(4936..5664)
                     /gene="ccoO"
                     /locus_tag="VF226_21015"
     CDS             complement(4936..5664)
                     /gene="ccoO"
                     /locus_tag="VF226_21015"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEX7076527.1"
                     /translation="MNHAVLEKNSILLLIGILIVVSIGGIVQIAPLFWIDSTIEKVQG
                     MRPYTPLEQAGRDIYVREGCYTCHSQMIRTLRDEVERYGHYSLAAESMYDHPFQWGSK
                     RTGPDLARVGGKYSDQWHVEHMMNPRSVVPESIMPAYPWLAENKLDASTIADRLRTLR
                     AVGVPYTDEMIEKAEADLKAQVDPDSREARELLQRYPKAKVAKFDGKPGGQVTELDAL
                     VAYLQMLGTLVDFTTFDAAGPNLR"
     gene            complement(5680..7338)
                     /gene="ccoN"
                     /locus_tag="VF226_21020"
     CDS             complement(5680..7338)
                     /gene="ccoN"
                     /locus_tag="VF226_21020"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEX7076528.1"
                     /translation="MADTAVERRAFSDVVAVLGCGLGILLGLLLATGTHDAVMSFHGA
                     LLALACAAGAIYVLKDTFGLEDKPETEEGYFDGPIKVAIVAAVFWAVAAFAVGDLIAW
                     QLAFPALNFDLPWTSFGRMRPLHTSAAIFAFGGNVLIGTSLYVVQRTCHARLAGRWAP
                     YFVVWGYQLFILIAGTGYLLGVTQSKEYAEPEWYADLWLTIVWVVYLLVFLGTIARRR
                     EPHIYVANWFYLAFIVTIAMLHIVNNLALPVSLTGTQSYIVFSGVQDAMTQWWYGHNA
                     VGFFLTAGFLGIMYYFIPKRVERPVYSYRLSIVHFWSLIFIYIWAGPHHLHYTALPDW
                     AQTLGMTFSIMLWMPSWGGMINGLMTLSGAWDKLRTDPVVRLLVVSVAFYGMSTFEGP
                     LMSIKAVNSLSHYTDWTIGHVHSGAIGWVAMVSFGAIYCLAPWLWGRRGLYSLRLVEW
                     HFWISTIGILLYITAMWVSGILQGLMWRAYDSLGFLQYSFVETVEAMKPFYMIRATGG
                     LLFLIGSLIMAYNIWRTIRGDEPVDAMEQPRVAAAPELRLVPAE"
     gene            complement(7511..8002)
                     /locus_tag="VF226_21025"
     CDS             complement(7511..8002)
                     /locus_tag="VF226_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076529.1"
                     /translation="MTKSGEAKARYALGLFVSADQLERALTELRETGLPLAQIKVIMP
                     PLSSGANPSWSERAAALGADTWVASESANRRGDSGVHCPWDFAPVHLSQRESATKRDV
                     MPDFHIWALERHAQQLDRHLRRGGAIAIVEVRNDGEERAAYSALLRHATAGVQTHEVS
                     RHS"
     gene            complement(8074..8775)
                     /locus_tag="VF226_21030"
     CDS             complement(8074..8775)
                     /locus_tag="VF226_21030"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7076530.1"
                     /translation="MLALVSPSENISRDFDQPSHHRPAHADFVDGWPQLGSVQSVPAR
                     EEIFFDGDEARNVFEVLDGIVCAYRILPDGERHIVSFYYPGDVIGNCTLGTYAYSAQA
                     LTPARVRRIPRTVLERSMAQRPELAQRFLRLAAIELAATRDHLLCLAAKSAEAKLAAF
                     LLALSRRNQEQGEDPDRITLPMTRLDIADYLGLTIETVSRTLSKFRRNGLIDLPRATT
                     VDLKDADRLAFLADA"
     gene            8912..9664
                     /locus_tag="VF226_21035"
     CDS             8912..9664
                     /locus_tag="VF226_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7076531.1"
                     /translation="MPEIAMNKVPAHILVVDDEPQVRRLLRRCFEGEGYKVTEAGSSA
                     EVIEHLKRASFDLVTLDLNLPDIDGLTLAREIRSRSPVPIIMVTGKGDTIDRVVGLEL
                     GADDYITKPFHLREVLARVRAVLRRSAAASGAAADAPAPDGDTLTFDGWELSPAKREL
                     KKPCGTPCDLTTSEFDLLVLLANNANRVLSRDQIMDRLKGHDWTPYDRTIDNLIVRLR
                     RKIEPDPERPTLIKTVRGVGYTLTADVTRGRG"
     gene            9859..10611
                     /locus_tag="VF226_21040"
     CDS             9859..10611
                     /locus_tag="VF226_21040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076532.1"
                     /translation="MRFQIALLCILSMTACAPQKDSISLTAAPGQQAIIRDGVPALVS
                     SKKNIVFLRPLDARQESGGRPKFVVAMYNRGNAPATFRMSDLRVEMLQPRHASIKVYT
                     HAELADEVETERNTRLFLAALSGAAGAVSAASAGTTYTSGSYGYSTPYGTGSGFYTGS
                     SYNPGLAQAAINANNDRTLNTMASIESSAQNALTTLRATILKDHTLMPNEWHGGVIVL
                     DPPEMSKEGAQYQISLWLDGEEHVFTVSQRSS"
     gene            complement(10704..11762)
                     /locus_tag="VF226_21045"
     CDS             complement(10704..11762)
                     /locus_tag="VF226_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7076533.1"
                     /translation="MQQDATNYGSFGPEPIETDCVIIGAGPVGLFAVFELGLLDIRAH
                     VVDILDKPGGQCAELYPDKPIYDIPGLPVVTGQQLTDQLLEQIKPFNPTFHLGERVDE
                     LKRLPDSRFLLKTDAGTTFLTRVVVIAAGGGSFTPKRPPLDGIEEFENKSVFYAVRRM
                     QDFAGTDLLIVGGGDSALDWTLNLQPLAKSLTLVHRRDEFRAAPHSVERMRALVAEGA
                     MQLKIGQLQALHGTDGWLEAVTIRTKAGEERLACNRLLPFFGLTMKLGPIADWGLNLN
                     ENLIPVDTEKFETSEKGIFAIGDINTYPGKLKLILSGFHEAALMAQQAHRYVYPDRKL
                     LFQYTTSSTSLQKKLGVR"
     gene            complement(11880..12200)
                     /locus_tag="VF226_21050"
     CDS             complement(11880..12200)
                     /locus_tag="VF226_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7076534.1"
                     /translation="MVKITFVQPDGSKQVVDATPGMTVMEAAKLALVPGIEAECGGAC
                     ACATCHVYVDSEWRDKTGEPSQMEEDMLDFAFDVREESRLSCQIKVTEELEGLVVYVP
                     EKQF"
     gene            complement(12464..12895)
                     /locus_tag="VF226_21055"
     CDS             complement(12464..12895)
                     /locus_tag="VF226_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX7076535.1"
                     /translation="MAEPARSNIDLDPRATKSDAQRESAALPAGDSEDRLAPIDFDHL
                     RRYTLGDARLEREVLELFCSHAPVLVAELRKAVSDRAWRDAAHSLKGSARAIGAWEVA
                     RAAELAELAAVPQRERPELVSRLEAAVQRVQRFMMARRAIS"
     gene            13214..16438
                     /locus_tag="VF226_21060"
     CDS             13214..16438
                     /locus_tag="VF226_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076536.1"
                     /translation="MAQDPKVKRDKAPDLSLVGSTPATPKGVASDKDKATPPGPPPLP
                     PSASVGTSAAAKAVHVERDRNEPIPLPQSPSSDAKRDTPPNPKLADKPEATKESGRKL
                     TPPPPPPPSGPPPASADSAKVVRPLKLSELQRRHEPPRPPENATPAKPQAGPAPAKAP
                     EPQGQAAKEPESKVPSDAQRASGPSAAAGAQNAGTEDVAERRPARRRPAGPSRARIAA
                     NDDMPSIGGLIYALNERPSNKPFVYAAAASGVWAAVAIAFAWAFISRELADATGFIDA
                     IGRPATITAFATVVGPVALFWFLALLVWRTEDLRLRSTAMTEVAVRLAEPDRMAEQSI
                     ASLGQAVRRQVSFMNEAVSRALGRAGELEALVHNEVTALERSYEDNERKIRSLIQELS
                     GERNALLNTTDRVNEALKSIASEVPVLIEKLSNQQVKLATIIEGAGQNLTALESALSE
                     QTGRLEHTLGSRTQDMQAVLHEYTAALGSALGSRTEQLQAVLEQYTASFGEALSGRAE
                     HMQKLFEDYKKSIESSMANRTENLQTVFEEYARALDTTLANRAQALDMQLVQRTAALD
                     QAFNERLKAFDESILRSTMAIDSAIGEKTEALTKALENHAHGLRDTLGQQAIELDETL
                     MQGIAAVRRTSENITRQSVKAIEGLSSQSEMLKNISENLLAQIHSVTNRFESQGQSIM
                     MAANSLEAANYKIDATLQSRHEQLSETLERLTGKAEEMSQLMYGASSSLEGSLTQAEQ
                     RARQLTQELTKGAEARSRSAIAEIERMKLAANVEADRAFDELISRFSRVSRQVTEQLA
                     QLQTQFNATSSEVRMQAQRAAAELASEQARLRKMIESLPHATREGSEIMRKALSDQVR
                     ALEELTNLTAREAARRDAMPAREPIPGGALTPISPPQERQRTLSSLTSTLADELGQRQ
                     RKAAAINSASGAESSPADSRDGWSLGDLLARASREDESGLRHQQPPPAPAPLSIDVDL
                     LSRALDQATASAIWSRFRAGQRGIMVRSIYSNEGRAAFDEVSRRYRTDPAFQNMVDRY
                     LDEFEQSLRETERQEGSARLLASQLVSDTGRVYLFLAHASGRLS"
     gene            16471..>17254
                     /locus_tag="VF226_21065"
     CDS             16471..>17254
                     /locus_tag="VF226_21065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEX7076537.1"
                     /translation="MEKLKIEAAIESWPLASDFVISRGAKREARVVVVRLSDGRVTGR
                     GECVPYGRYGQTPEATLAQIRSAPADLDRCSLQHSWPPGAARNALDCALWDLEAKRSG
                     RSAAELAGLAPMRPVLTCYTLSLDTPEAMAAAARSVPHLPLLKLKLGAPGDADRMRAV
                     RQARPDARLVADANEGWQPSDLGHLMEVAAEAGLELIEQPLPAGDDGALATVARAVPV
                     CADESAHTTDGLLALRDRYDAVNIKLDKTGGLTEALRMAREAR"
BASE COUNT         3226 a         5683 c         5440 g         2905 t
ORIGIN      
        1 cgtgcgtagg gatggcggct cgcagcagca agggccgccg cgcggatcaa taggtcatcg
       61 ctgcaggatt gcggctcgat gagccgtggc tcgcccagcg tgagcgtacc cgtcttgtcg
      121 aaaacgatgg tgtcggtttc agccaatcgc tccagcccgt ctgccgcttt cacgatgaca
      181 ccgcggccga acagacggct tgtcgccgcc acctgcacgg ccggcacggc gagcgccagc
      241 gcgcaggggc aggtgatgat gagcacggag atggcagccg tcagcgcagg ctcccagcca
      301 tggcccatta tcagccatcc gacgaacgtc atgaggccca gcgaatgcac tgcgggcgcg
      361 taatagctcg cggctcgatc ggccagccgc acgtagcgtc ccctgccctg ctcggcggcg
      421 gtcatcagcc ggccgatctc ggccagcagc gtgctttcgt tggtggccgt cgcctcgatg
      481 acgagcggcg cgcccatgtt cagcgttccc gcgaacactt ggcgccctgg ttccgccgta
      541 accggctcgg cctcgccggt aataaggctc tcgtcgatgg ctcctctgcc ctcgacgatc
      601 ttcccgtcgg cgccgatccg ctcgcccgtc gcaacgagaa tgcgcatgcc gggggtcagc
      661 gcacgcgccg acagacgttc cgtgctgcca tccgggaaca gcacggtcgc cgtgcttccg
      721 cgtagcccca ggagattggc ggcagcaccg gccgcccgcg tgcgcatgct ctgatcgaga
      781 aagcggccga tgagcaggaa gaacagcagc gacacggcgg catcgaaata gacgtgctcg
      841 ctgccgcgga tcgtctggaa gaggctcata gcggtcgcca gggtcacggc gagcgagatc
      901 ggcacgtcca tattgagccg gcgtgatctc agagcctgca tggccgaacg gaagaacggc
      961 tggccggcgt aagcgactgc aggcagcgcg atgagcgcgg aaatccagtg gaagagcgtc
     1021 tgaaccgact gctccatgtc gccggcctgg cccgaccaga cggcgacgga cagcagcatg
     1081 atgttcatcg ccgcgaagcc cgccacgccg acgcggcgca ggaactcctt gtcggcggac
     1141 ttgtcgctcg cagcgtcttc gagcaacggc gcggccttgt agccggcctt gccgagaaca
     1201 tcgaccaggt cgtcggtcgt gacccggccc ttgctgctcg tcacggtcac gcgccgggcg
     1261 gagagattgg cacgtgccga aatcacgccg gggaattcgg cgagcgcctt ctcgaccttc
     1321 cgcatgcaac cgccgcagtg catgttctcg acgaccagcg tcgttgattc acatgcggaa
     1381 ttgccttgcg agctgccagc agccgccgtc tcgtgcgggg ccgcttccgt caggacttga
     1441 gccacagcct tttcctcaac ctgtaaactg gctccgtttc acccgtgccg ggccaaatcg
     1501 ctttgaggtc gaccagccac gcgccagggg acagcgcttc gacgcctgcc acatagcgcc
     1561 ccggctccac ctcctggagt acgagctgcc tgtcgtattc gacggtggag gggcgaccca
     1621 ggatgccgga gacgcgcagg ccggaaaccg gaccgccgtc cgccttgcgc agcaccagcg
     1681 tcaatcctgc agcgggatcg agcgacgtct ccgccgtcca gccgagctgc tcctgccgtt
     1741 cagcggcagc gatgcgatca ttgtaccgca gccccttccg gtaaggctcg tcggcgacca
     1801 cgccagtata ggttgacaga gcggcgtaga tgaaaatgcc gttggcgaga aacaccgttc
     1861 cgaaaaatgc gagcaccgcg atcagcacat ggcggccgtt gagaccctgc ctcacttgcc
     1921 gggctgacat ctctgcacct ttcgtcctca tcactagctc cgccccgatg cgctctggaa
     1981 gatggcatca cggcgcatct cggtgcccgt tgcgacgtcc ttcacgacga aatcgaaccg
     2041 ggtggatggc ggcgtgagct gccccagatg ctcgggcggc acggtcacca ggacacggac
     2101 ctcgcgcaga ttgtctgccg gaatgctgag cgccccgtcg ccagcatcat gcccgacgat
     2161 ccgcatggct gcgccctgca gcccatcgat gctcagcacg acgtccaccg gcacatgctg
     2221 cttgttgagc accttcaccg tgtaaccatt gcgcacgccc ccatccgaca atggcacgaa
     2281 cagcacgccg cgatcgtgca gcacgttcac ctcaagcgtc gtccgggaca cgaggccgag
     2341 ggccatgacg acggcgacca gcgcgatcag cccggagtag aggatcgtgc gcgggcggac
     2401 cagcttcagc ggctcatgga cgacgccctt agccttcgcc tcctgcttgg caaccgtgtc
     2461 gtagctgatg agaccgcgcg gacggccgat cttgtccatg atctcgttgc aggcgtcgat
     2521 gcacagcgca cattggatgc actcgaactg cgcgccatcg cggatatcga tgcccatcgg
     2581 gcacacagcg acgcaggcct tgcagtcgat gcagtcgccg cggccttccc agccctgacc
     2641 tttgcggagg ggcccgcgcg gctcgccgcg ctcgggccgg tagctgacga gcagtgtgtg
     2701 ccggtcgacc atcgcgccct gaatgcgcgg ccacgggcac atatagatgc agacctgttc
     2761 gcgcgcgatg ccgccgagga gataggtcgt gaacgtaaag atgccgagga acaggtaagc
     2821 gatggccggc gcggttccgg tcaccagctc cgccgcgagc gtcggggcat cgcgaaagta
     2881 gaagacgagc gcaccaccgg tcgcaaggcc gattgccagc cagctcaggt gcgtcatcac
     2941 cttcttgaag atcttggtcg cgctccacgg ctccttgtcg agccggatgc gcgcattacg
     3001 gtcaccctgc cagaaccgct ccacggccag catgagatcc gtccacaccg tctgcggaca
     3061 ggtatagccg caccagacgc gacccgcggc cgccgtgacg aggaaaagac tgagggcgga
     3121 gatcacgaga atgccggtga tatagtaaag ttcctgcggc cagatctcga ggaaaaacag
     3181 gaacagccgg ccattggcga aatcgagcaa tacggcttga tcgggcaaat tcggaccgcg
     3241 atcccagcgc agccacggca gcgcgtagta gatggagagc gtgatcgcca tcaccgccca
     3301 tttcaccgtg cgaaagcggc cccgagcgag acgcggatag atcttctgcc gtgtcgcata
     3361 gagctggcgg cgatccttgc ggttgatcgc ggctacatcg agcttctcga cgccaggcgc
     3421 ggcctcgctg atgaagtcgg ctggcttcag atcctgcggc atagacgtca tcgctctccc
     3481 ttgcctcctt agccggagga gtggtggtga gggacagctt ccttcgggcg ctgcatcatc
     3541 cgcccctcac ctgccctcgc cagctgacca cgagcgaggg aactactctt cgtgctcgga
     3601 cagtgcgcct tccgggattg ccaggttctc ggataacgcg ccttccgtgt tgccagtaag
     3661 gggagcgagt atcgactttg acgcactatt cgatcccgtt gatcccctca ccctaaccct
     3721 ctccccatgg gagaggggac tatgccttac gtgcggcaag cgcgacgcca agtcgagagc
     3781 gctctgttgc cgcttgcgct ttctccctct ccccgatgcg aagagggtcc gcgtgcgggg
     3841 gaactgcgcc agctgtccgt gcagaccctc tcacccgcgc tgtcactgcc ctcctccgag
     3901 cgaatggacg tacacggcca gcgacttgat cgtcaccgga tcgaggcggc cttcccagac
     3961 cggcatctga ccgccgcgac cggtgcggat gctctcgacg acggcttcct tcgaattgcc
     4021 gtagagccag atcgcgtccg tcaggttggg cgccccaaac tcgcggttgc ccttggcgtc
     4081 ctctccatgg caggagacgc actgctcggc gaatatctca ctcccgcgtg cgacagcgtc
     4141 ctggtccgtc gcccgaccag tgagcgacag cacgtactcc gcaacatcgt tgatctgctt
     4201 gcgctcgagg aggccatcga taccgaacct cggcatctgg ctgtcatggc cgtccgcgtg
     4261 gcctgagcgg attccgaacc ggatcgtgtt gtggatctcc tcgatgctgc cgccccacag
     4321 ccaatcgtcg tcattgaggt tcgggtagcc gacgaaccct tgtgcgccgc ggccgtggca
     4381 gggcgcgcag ttggtcgcga aggctgccgc tcctccggcc atcgcgaagc gcaggagctc
     4441 cgaatcgtcc ttcacccgct ccagaggcgt cgaagcgagc gcctcgcgga atttgccttg
     4501 cgctgcgcgc gcttcggcga cctgattggc cacggcctga cgttggctgt agccgagcac
     4561 gcccttggta tagtcggcca tggtcggcca cgcgggatag aggatccaat agccgatcga
     4621 ccagatgatg gtcgcgtaga acgtccagac ccaccacttc ggcagcggct tgttgagctc
     4681 ctttatgccg tcccactcgt gacccgtggt ctcgatgccg gtgacctcgt cgacttcctt
     4741 cctgctcatt tgcagccccc gcgggaattg tggtccttat cgagatcgag cgcctgtttc
     4801 tgcgcgatct cgaagcgatt gcggttgccc ggccagcagg catagacgac gacgccgagg
     4861 aagatcccga tgaagaggag gagcgagctc acctggctga agctcgcgac ggtttcgtag
     4921 gtcatcgctc agccctcacc tcagattggg accggcagcg tcgaacgtcg tgaagtcaac
     4981 gagcgtgccg agcatctgca agtacgcgac gagcgcatcg agctctgtga cctggccacc
     5041 gggctttccg tcgaacttgg caaccttggc cttggggtag cgttgcaaaa gctctcgcgc
     5101 ttctcgcgaa tccggatcga cctgggcctt cagatcagcc tcggccttct cgatcatctc
     5161 gtccgtgtaa ggcacgccca ccgccctcag tgtcctcagc cggtcggcga tggtgcttgc
     5221 atcgagcttg ttctcggcga gccatgggta agctggcatg atgctctcgg gcacaacgga
     5281 ccgtgggttc atcatgtgct cgacgtgcca ctggtcggag tacttgccgc cgacgcgggc
     5341 cagatcagga ccggtgcgct ttgagcccca ctggaacggg tggtcgtaca tgctctccgc
     5401 ggcgaggctg taatgcccgt accgctcgac ctcgtcgcgc aaagtccgga tcatctggct
     5461 atggcaggtg taacagcctt cccggacgta gatatcccgg ccggcctgct cgagcggggt
     5521 gtagggccgc atgccctgca ctttctcgat cgtgctgtcg atccagaaca gcggcgcgat
     5581 ctgcacgatg ccgccgatcg agacgacgat caaaatgccg atcagcagaa ggatcgagtt
     5641 cttctcgaga actgcgtggt tcatgtcgcg ggctccccct tattccgcag gaaccaggcg
     5701 caactcgggc gcggccgcga cgcgcggctg ctccatcgca tccaccggct cgtcgccgcg
     5761 aatggtgcgc cagatgttgt aggccatgat cagcgaaccg atcaggaaca gcaggccgcc
     5821 ggtcgcgcgg atcatgtaga agggcttcat cgcctcgacg gtctcgacga acgagtactg
     5881 caggaagccg aggctatcgt aggcgcgcca catcagaccc tgcaggatgc cggacaccca
     5941 catggcagtg atgtagagca ggatgccgat cgtcgagatc cagaagtgcc attcgacgag
     6001 gcggagcgag tagagacccc gacgccccca aagccagggt gccaggcagt agatggcgcc
     6061 gaaggacacc atggcgaccc agccaatcgc gcccgagtgc acatggccga tcgtccagtc
     6121 ggtgtagtgg ctgagggagt tcaccgcctt gatggacatc agcggcccct cgaaggtcga
     6181 catcccgtag aaggcgacgg agaccacgag cagccggacg acggggtccg tgcgcagctt
     6241 gtcccatgcg ccggagagcg tcatcaggcc gttgatcatg ccgccccagg agggcatcca
     6301 cagcatgatc gagaaggtca tgccgagcgt ctgcgcccag tccggcagcg ccgtgtagtg
     6361 caggtggtgc gggccggccc agatgtagat gaagatcagc gaccagaagt ggacgatcga
     6421 cagccggtag gagtagaccg gccgctccac gcgcttcggg atgaagtagt acatgatccc
     6481 gaggaagccc gccgtcagga agaagccgac ggcgttatgg ccgtaccacc attgcgtcat
     6541 cgcatcctgc acacccgaga agacgatgta gctctgagtg ccggtcagag agacgggcag
     6601 cgccaggttg ttgacgatgt gcagcatcgc aatcgtcacg atgaacgcga ggtagaacca
     6661 gttggcgacg tagatgtgcg gctccctgcg ccgcgcgatc gtgccgagga agacgagcag
     6721 gtagaccacc cagacgatcg tcagccagag atcggcgtac cactcaggct cggcgtactc
     6781 cttgctctgc gtgacgccga gcagatagcc ggtgccggca attaggatga aaagctggta
     6841 gccccagacg acgaagtagg gcgcccaccg cccggccaga cgcgcatgac aggtgcgctg
     6901 aaccacgtac aaggacgtgc cgatgagcac gttgccgccg aacgcgaaaa tcgccgccga
     6961 cgtgtgcagc gggcgcatgc gcccgaagct cgtccacggc aggtcgaaat tcagggccgg
     7021 gaaggcgagc tgccaggcga tgaggtcacc gacggcgaag gccgcgaccg cccagaaaac
     7081 cgcagcgaca atcgccacct tgatcggccc gtcgaagtaa ccttcctcgg tctcgggctt
     7141 gtcctccaag ccgaatgtgt ccttcaggac atagatcgcg ccggccgcac aggcgagggc
     7201 cagaagagcg ccgtgaaacg acatcacggc atcgtgggtg cctgttgcca gcaaaaggcc
     7261 aagcaggatg cccaatccgc accccagaac tgcgaccaca tcggaaaatg cgcgccgctc
     7321 gactgccgta tctgccatct ccgtatccct cgtctcggcg atgatccatc agttccgtcc
     7381 acctacccgc tcgagccttg attttcgtca agacgcattg ccttaggtca accggtcgca
     7441 ccggacacga acatgtgcca tcgcagcgtg gtcgaggggc ttggtcttcc ttggccctgg
     7501 gttcgcggat ttatgaatga cggctgactt cgtgggtctg gacgccagcc gtcgcatgac
     7561 ggagcagtgc ggaataagcc gcacgctcct cgccgtcgtt tctgacctcg acgatggcga
     7621 tcgccccacc cctgcggaga tggcgatcga gctgctgggc gtggcgctcc agtgcccaga
     7681 tgtggaagtc gggcatcacg tcgcgcttcg tcgcgctctc gcgttgcgag agatggacag
     7741 gggcgaaatc ccacgggcag tgtacgccgg agtccccccg cctgttcgcg gattccgagg
     7801 cgacccaggt atccgctccg agcgccgctg cccgctcgct ccaggatgga tttgctccgc
     7861 tgctcagcgg gggcatgatc accttgatct gagcgagcgg cagtccagtc tcgcggagct
     7921 ccgtcagggc acgctccagc tggtcggcgc tgacaaagag gccgagcgca tagcgcgcct
     7981 tggcttcgcc gctcttggtc atgctcgttt catccccgcg gaccaccccg tcgggagcct
     8041 cgcccaaaga ctcgaaccac gcattgatct ggatcaggca tctgccagga acgccaggcg
     8101 atcggcatcc ttcagatcga cggtcgtcgc gcgcggcagg tcgatgagac cgttccgccg
     8161 gaacttgctc agcgtgcggc tgacggtctc gatggtcagg ccgaggtagt ctgctatatc
     8221 aagcctggtc atcggcaacg tgatccgatc cggatcctcg ccttgctctt gattgcgccg
     8281 ggacagggcc agcaggaagg ccgcgagctt tgcctcggcc gacttcgccg caagacacag
     8341 gagatgatcg cgggtcgcgg ccaactcgat cgcggcaagg cggaggaagc gctgggccag
     8401 ctcgggccgc tgagccatgg agcgctcgag aactgtgcgg ggaatgcggc ggacgcgagc
     8461 tggcgtaagg gcctgtgcgc tgtaggcgta tgtccccagg gtgcagtttc cgatcacgtc
     8521 gccggggtag tagaaggaga cgatatggcg ctcgccgtcc ggcagaatgc ggtaggcgca
     8581 gacaatgccg tcaagaacct cgaatacgtt gcgcgcctcg tcgccatcga agaatatttc
     8641 ctcacgagcc ggaacactct gaaccgagcc gagctgcggc cagccatcca cgaagtcggc
     8701 gtgcgcagga cgatgatggg agggctggtc gaaatcgcgg gagatgttct ctgaaggtga
     8761 tactaaggca agcatcgaac cgatcctctc gtcgaccttg ttgataggat gcagttcagc
     8821 aggggcatgt catacccgca tgggaactgt gtaagagttt gtaagagcgt gtcgaacgat
     8881 gctgcgtccg ggcgacaaaa ggggccccga gatgcctgaa atcgcgatga acaaggtccc
     8941 agcgcacatc ttggtcgtcg atgacgagcc tcaggtccgg cggctcttgc gccgctgctt
     9001 cgagggcgaa ggctacaagg tgaccgaggc cggcagcagc gcggaggtga tcgagcacct
     9061 gaaacgggcg agctttgacc tcgtgacgct cgacctcaac ctgccggata tcgacggctt
     9121 gacactcgcg cgcgagatcc gctcacgctc gccggttccg atcatcatgg tgactggaaa
     9181 gggcgacacc atcgaccggg tggtggggct cgagctcggc gccgacgact acatcaccaa
     9241 gccgttccat cttcgggagg tcctggcgcg cgtccgcgcg gtcctgcgcc ggtccgcggc
     9301 cgcatcggga gccgcggctg atgccccggc gccggacggg gacacgctta ccttcgatgg
     9361 ctgggagctc agccctgcca agcgagagct gaagaagccc tgcggcacgc cctgcgacct
     9421 gacgaccagc gagttcgacc tgctggtgct ccttgcgaac aacgccaacc gggtgctgtc
     9481 gcgggatcag atcatggaca ggttgaaggg gcacgactgg acgccctacg accgcaccat
     9541 cgacaatctc atcgtgcggc tgcggcgtaa gatcgagccc gatcccgagc gcccgacgct
     9601 catcaagacc gtgcgcggcg tgggttatac cctgacggcc gatgtcacgc gcgggcgcgg
     9661 ctaagatgag gcggaaaagc gattacgaaa tgaaatcaac ctacgcgtga ggcttgttgc
     9721 acggccatca gccagcgata gcgatccgct aattttgcgt gaattgctca atcatcatca
     9781 aatttgctgc tgggctgccg cttgcctgac gcatacagac tcgtgatctg tatacggaat
     9841 cggggctggg gggtagatat gcgatttcag atcgccttgt tgtgcatttt gagcatgaca
     9901 gcttgtgcgc cgcaaaagga cagcatttcg ctgacggcgg cccccggcca acaagccatc
     9961 attcgggacg gggttccggc gctcgtctcc tcgaaaaaga atatcgtctt tctccgcccg
    10021 ctcgatgctc gccaggaatc tggtggccga cctaagttcg tggtggcaat gtacaatcgc
    10081 ggcaatgcac cagccacgtt ccgaatgagc gatttgcgag ttgaaatgct tcagccgcgt
    10141 catgccagca tcaaggtcta tacccatgcg gagctggccg acgaggtcga aaccgagcgc
    10201 aatacgcgcc tgtttctggc cgctctgtca ggtgcagctg gcgccgtttc cgcggcatca
    10261 gccggaacga cttacacaag cggctcatac ggctatagca cgccttacgg aactggctcc
    10321 ggattctaca caggcagctc ctacaatcct ggactcgcgc aggcagcaat caacgccaac
    10381 aacgaccgaa ccttgaacac gatggcctcg atcgagtcga gcgcccagaa tgcactcacc
    10441 accttaaggg caacaatcct gaaagatcac acactcatgc ctaatgagtg gcatggcggc
    10501 gtgattgtgc tcgatccgcc agagatgagt aaggaggggg cccagtacca aatttccctc
    10561 tggcttgatg gcgaagagca tgtcttcacc gtcagccagc gcagctcatg agcaggcggc
    10621 ggtgtcgctc gcgagtcaac aaagtagcct gctgttgcgc tttagccgga agtcgccgat
    10681 agacccgttc cttaggcccg aattcagcgc accccgagct tcttctgcag gctcgtggac
    10741 gaggtcgtgt actggaacag cagcttccgg tccgggtaga cgtacctgtg cgcctgctgg
    10801 gccatcagcg ccgcctcgtg gaagcccgac agaatgagct tcagcttgcc cggataggtg
    10861 ttgatgtcgc cgatggcgaa gatgcccttc tcgctggtct cgaacttctc cgtgtcgacc
    10921 gggatcaggt tctcattcag gttcaaaccc caatcagcga tcggcccgag cttcatggtc
    10981 aagccgaaga acggcagcag ccggttgcag gcgagccgct cctcgccggc tttcgtgcgg
    11041 atcgtgacgg cctccagcca gccgtccgtg ccgtgcagcg cctggagctg gccgatcttg
    11101 agttgcatgg cgccttcagc caccagcgcc cgcatccgct ccactgaatg gggcgcagcg
    11161 cgaaactcgt cacgccgatg aaccagcgtc aggctcttgg ccagcggctg caggttcagt
    11221 gtccagtcga gcgccgaatc gccgccgccg acgatcagca ggtcggtgcc ggcaaagtcc
    11281 tgcatccggc gcaccgcgta gaacaccgac ttgttctcga attcttcaat gccgtcgagc
    11341 ggcggacgct tgggcgtgaa ggagccgccg ccggcagcga tgaccaccac ccgcgtcagg
    11401 aacgtcgtcc cggcgtcagt cttcagcaga aaccgactgt ccggcagccg cttcagctca
    11461 tcgactcgct cgccgaggtg gaacgtgggg ttgaacggct tgatctgctc gagtagctga
    11521 tctgtcagct gctggccggt aacgaccggc agccccggga tgtcatagat cggcttgtcg
    11581 ggataaagct cggcgcattg gccaccgggc ttgtcgagaa tgtcgacgac gtgcgccctg
    11641 atgtcgagga ggcccagctc gaacacggcg aagagcccga caggccctgc tccgatgatg
    11701 acgcaatccg tctcgatcgg ctccgggccg aagctgccat agttcgtcgc gtcctgttgc
    11761 acgtcgctag ctcccgatca tctgcgttcg atgcaaaccg gcctcgcgtg catcagaacg
    11821 cccctacgca ccgctcgcca ttgcctccgt gacaaccgta cccgagtgtg cggtgagcgt
    11881 cagaactgct tctcgggcac gtacacgacc aagccttcca gctcctcggt caccttgatc
    11941 tggcagctca ggcggctttc ctcgcggacg tcgaaggcaa agtcgagcat gtcctcctcc
    12001 atttgggagg gctcgcccgt tttgtcacgc cattcgctat caacgtaaac gtggcaagtg
    12061 gcgcaggcgc aggcaccgcc gcactcggcc tcgatgccgg gaaccaatgc gagcttggcc
    12121 gcttccatga cggtcatgcc aggcgtcgca tcaacgacct gcttagaccc atccggctgg
    12181 acgaatgtga tcttgaccat gcgtgcgccg tgctccctgg cagcgtgtat ccaagtgcgt
    12241 cagctgaagt tcgggaacgg tggtgacgac gcactccccg gctttccagc gtggagccgc
    12301 ctcgcgccct gactcgcgac catcgtaagc ggcgaattga tagacacccg gggtcatgaa
    12361 ttcaagcgca ctgccgacgc agggccgctc ccatgacggc aggagagccc caagaaggaa
    12421 gccagaatgg cggtccgcca ccgccgccac cacgacggct cggtcaggag atcgcgcggc
    12481 gcgccatcat gaaccgctgg acgcgctgga cggctgcttc cagccggctg accagctcgg
    12541 gccgctcacg ttggggcacc gccgcgagct ccgcgagctc tgcggccctc gccacctccc
    12601 acgccccaat cgcccgcgcc gagcctttca ggctgtgcgc ggcatcacgc catgccctgt
    12661 cgctgaccgc cttgcggagc tctgcgacga gcacgggcgc atgactgcag aacagctcca
    12721 gcacctcccg ctccagccgt gcgtcgccaa gggtgtaccg gcgcagatgg tcgaaatcga
    12781 tgggcgccag ccgatcctct gaatcgccgg ccggcaaagc cgctgattcg cgttgtgcat
    12841 ccgacttggt tgccctggga tccaggtcga tgttcgaccg agcaggttca gccaccacgg
    12901 cctcccctca agaagagaga tactcatcca gaacacatcg agatcttatc agtgtggcga
    12961 ataagcccag gtattcgtaa cactgtgcta tttacaggat atctcaacca cttgattacc
    13021 atctcatcat cggtcgtcca tcgttttgcc caaaatgcgg actaccgacg gctcgagggc
    13081 gagactgtgg cgaatgtgta acgcctcaaa acggcacgcg ttgagaaaat gccatatagg
    13141 tcttgtccgc cgtatgcact agacaagtgc aggtgggggc gccttcgggc gcgaggggac
    13201 gtcggacaat tgaatggctc aggacccgaa ggtcaaaagg gacaaggcac ctgacttgtc
    13261 cctggtcggc tcgacaccgg ctacgccgaa aggagttgcc tcggacaagg acaaagcaac
    13321 tccgcctggt ccgcctccgc tgccgccatc cgcctccgtt ggaaccagcg ccgctgcgaa
    13381 ggcggtgcac gtcgagcggg accgcaacga gccaattccg ctgcctcaat cgccgtcttc
    13441 agacgcaaag cgcgacacgc ccccgaaccc gaagctggcg gacaaacctg aagcgaccaa
    13501 ggagagtggt cgcaagctca ccccgcctcc gccgccacca ccgtccggcc cgcccccggc
    13561 gtcggccgat tcggccaagg tcgtacggcc gctgaagctt tcagagttgc agaggcggca
    13621 tgagccgcct cggccgccag agaacgcgac accggcaaag ccgcaagccg ggcccgcccc
    13681 tgcaaaggcg cccgagccgc aaggccaagc ggcgaaggag ccggagagca aggtcccctc
    13741 ggacgcacag cgcgcgtccg gtccgtcggc tgcggctggc gctcagaacg ccgggaccga
    13801 agacgtcgcc gagcgccggc cggcccggcg gcgccccgcc ggtccctccc gcgcgcgcat
    13861 cgccgccaac gacgacatgc cgtcgatcgg tggcctgatc tatgcgctca acgaacggcc
    13921 gtccaacaag ccgttcgtct acgcggcggc ggcgagcggc gtatgggctg ccgtcgccat
    13981 cgcgtttgcc tgggccttca tcagccgtga gctggccgat gcgaccgggt tcatcgacgc
    14041 catcggccgc ccggcaacga tcacggcgtt cgcaacggtc gtcggacctg tcgcgctgtt
    14101 ctggttcctg gcgctcctcg tctggcgcac ggaggacttg cgcctgcgct cgaccgcgat
    14161 gacggaggtc gccgtccgcc ttgccgagcc ggaccgcatg gccgagcagt cgatcgcctc
    14221 tctgggccag gcggtacggc ggcaggtgtc gttcatgaac gaggccgttt ctcgcgccct
    14281 cggccgcgcc ggggagctcg aggccctggt tcataacgag gtgacggcgc tcgagcggtc
    14341 ttacgaggac aacgagcgca agatccgcag cctcattcag gagttgtcgg gtgagcggaa
    14401 cgcgctgctc aacaccaccg accgtgtcaa cgaggccctc aagagcatcg ccagcgaggt
    14461 tccggtcctc atcgagaagc tgtcgaacca gcaggtcaag ctcgccacta tcatcgaggg
    14521 cgcgggtcag aacctgacgg cgcttgagtc ggcgctttcc gagcagaccg gcaggctgga
    14581 gcacacgctc ggctcccgaa cccaggatat gcaggccgtc ctgcacgagt acaccgccgc
    14641 actcggctcg gccctcggca gccgcacgga gcagttgcag gcagtgctcg agcagtacac
    14701 cgcgtccttt ggagaggcgc tgtcaggccg cgcagagcat atgcagaagc tcttcgagga
    14761 ctacaagaag tccatcgaga gctcgatggc caatcggacc gagaacttgc agaccgtgtt
    14821 cgaggagtac gcgcgtgcgc tcgatacgac gctggccaac cgcgcacagg ccctcgacat
    14881 gcagctcgtc cagcgcacgg cggccctcga tcaggccttc aacgagcgcc tcaaggcctt
    14941 cgacgaatcg atcctgcgct ccacgatggc catcgacagt gccattggcg agaagacgga
    15001 ggcgctcacg aaggccctcg agaaccatgc tcacggtttg cgcgacacac tgggccagca
    15061 ggcgatcgag ctcgacgaga cgctgatgca gggcatcgcc gccgtgcgcc gtaccagcga
    15121 gaacatcacg cgccagtcgg tgaaggccat cgaagggctg tcgagccagt ccgagatgct
    15181 gaagaacatc tcggagaacc tgctcgcgca aatccacagc gtcaccaaca ggttcgagag
    15241 ccaggggcag tccatcatga tggctgccaa ttcgctcgag gcggccaact acaagatcga
    15301 cgccaccctc cagagccggc acgagcagct cagcgagaca ttggagcggc tgacgggcaa
    15361 agccgaggag atgagccagc tcatgtatgg cgcctcctcg tcactggaag gctccctcac
    15421 ccaggcggag cagcgtgcgc gccagttgac ccaggagctg accaagggcg cggaagcgcg
    15481 ctcgcgctcg gccatcgccg agatcgagcg catgaagctc gccgccaatg tcgaggcgga
    15541 tcgcgccttc gacgagctga tctccagatt ctcgcgagtc tcgcgacagg tcaccgagca
    15601 gctcgctcag ctccagaccc agttcaacgc gacctcttcg gaagtgcgca tgcaggcgca
    15661 gcgggcggcc gcggagctag catcagagca ggcgcgcctg cgcaagatga tcgagagcct
    15721 gccgcatgcg acgcgcgaag ggtcggaaat catgcgcaag gcgctcagcg accaggtccg
    15781 cgcactggag gaactgacca atctcaccgc ccgcgaggcg gcgcgccgcg atgccatgcc
    15841 cgcgcgcgag cccatcccgg gaggcgcact cactccgatc tccccgcccc aggaacgcca
    15901 gcggactctc agctcgctca cctccaccct cgccgacgag ctggggcagc gccagcgcaa
    15961 ggcggccgcg atcaattccg cgagtggcgc cgaaagctcg cctgccgaca gtcgcgacgg
    16021 ctggtcgctc ggcgatctac tggcccgcgc ctcgcgcgag gatgaaagcg gcctgcggca
    16081 tcagcagccg ccgccggctc cagcgccgct ttccatcgac gtcgacctcc tgtcgagggc
    16141 gctcgaccag gccacggcat ccgccatctg gtcccggttc cgggccgggc agcgcgggat
    16201 catggtgcgc agcatctatt cgaacgaggg ccgggccgca tttgacgagg tcagccgccg
    16261 ctacaggacc gacccggcct tccagaacat ggtggaccgc tacctcgacg agttcgagca
    16321 gtcgctgcgc gagacggaac gccaggaggg cagtgccagg ctgctcgcca gccagctcgt
    16381 atcggacacc gggcgcgtct acctgtttct cgcccacgcg agcggccgtc tctcttgacg
    16441 gccgccctgc ctgcgcgcaa gtgcgtcggc atggaaaagc tgaaaatcga agccgccatc
    16501 gagagttggc cgctggccag cgacttcgtc atctcccgcg gggccaagcg ggaggctcgg
    16561 gtcgtggtcg tgaggctctc ggatgggcgt gtcaccggcc gtggcgaatg cgtgccttat
    16621 gggcgctacg ggcagacgcc cgaagcaacc ttggcgcaaa ttcgctctgc gcctgctgat
    16681 ctcgaccgct gtagcctcca acacagctgg ccccctggtg cagcgcgcaa cgcgctcgac
    16741 tgcgcgctct gggacctcga ggcgaagcga tccggccgat ccgctgcgga gctcgcgggc
    16801 ctcgcgccaa tgcgccctgt cctcacatgc tacacgctga gcctcgacac cccggaggcg
    16861 atggcagcgg ccgcgcgcag cgtcccgcat ttgccgctct tgaagctgaa gctcggcgcg
    16921 cccggagacg ccgatcgcat gcgcgccgtg cgacaggccc gtccggatgc acgtctcgta
    16981 gccgatgcca atgaaggctg gcagccgtcg gacctggggc acctgatgga agtcgccgcc
    17041 gaggccgggc tcgagctcat cgagcagccg ctgccggccg gtgacgacgg cgcgctcgcg
    17101 actgttgctc gggccgttcc ggtctgtgcc gacgagtcgg cccacacgac agacggtctt
    17161 ctcgcgctcc gggaccggta cgacgcggtc aacatcaagc tcgacaagac cggcgggctg
    17221 acggaggcgc tgcgcatggc ccgggaagcc cggc
//
LOCUS       DASVDU010000315        11783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_787306, whole genome shotgun sequence.
ACCESSION   DASVDU010000315 DASVDU010000000
VERSION     DASVDU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787306"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="VF226_21070"
     CDS             complement(<1..1698)
                     /locus_tag="VF226_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEX7076538.1"
                     /translation="MKFEFTHAGIVRLAVGVLGVSMALYHMWHIWVGAPEAILYRGTH
                     LFFAMSLCFLVYRRKSAQAGQTPTLLDYAFLVLSIVPIFYLFVEYDYVVNRIYYVDDL
                     STLDMVLGTVLTLMILEATRRALGAALPLTALVFLAYGLLVAQVEPMRLLDQLYMTTE
                     GIFGIPLAVSASYVLIFVMFGAFMERTGTGQLFMDFAMSLTGHTAGGPGKVSVFSSSL
                     FGTISGSAVANVMVDGPIAIPLMKRTGFRPPFAAGVEAVASTGGQIMPPIMGAAAFVM
                     AEFLQVGYGQVIIWAFIPALLYYLACFSAVHFEAKRHGIMGLPRSELPSLPRVMAERG
                     HLFIPVLTILAVMYSGYSAPLAALAGTLACFPVAFLRASTRGNVRLGIIIEAMIEGSR
                     NAVGIALACATAGIIIGVVTLTGLGIVFTQVVVGLAQETLLLALVLTMIAGIVLGMGM
                     PTTPAYIIMTSLLVPAIIKLGVIEPAAHMFAFYFAILSAITPPVALAVFAAAGLAKSE
                     LWASGIAAVKIGAAGFIVPFMFVYEPALLMIGDWPTILWSTVTASIGCVLLAAGLHGY
                     "
     gene            complement(1780..2727)
                     /locus_tag="VF226_21075"
     CDS             complement(1780..2727)
                     /locus_tag="VF226_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX7076539.1"
                     /translation="MLDKLKYALLAAGAAFALTSGAAQAEQIRLMTGPQGGSWIPLGG
                     QLKDIWEKAIPGLQVQSLPGAGVANVRAIETDKAEVGFGNTITTADAIRGNPPFKEPH
                     KKVCNVATLYPQYFQVVVPADAGINSVKDIKGKALTTQPRGNTAEMITQQLLKVYGLS
                     YSDMKTSFVSYTDSATQLKDNQAQMFTLGTLIPASAIMDIAAARDIKLLDLSDAIDGM
                     KKLNPGYTLIEVPAGTYPKQDKAVNVIGYATHIVAACSLPEETVYQMTKGIAENVPSL
                     AATSKAMASLTPKQMAQDVGVPFHPGAAKFYKEQGVEVK"
     gene            complement(2781..3716)
                     /locus_tag="VF226_21080"
     CDS             complement(2781..3716)
                     /locus_tag="VF226_21080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX7076540.1"
                     /translation="MKTTQVTPADIARSVIAVPPLARDSELRLNRPANLAIIKHLESA
                     GIRSLMYGGNAVFFNISIGEYAEILDFLAESVGPDTWLLPSAGPDFGKLRDQAAILKS
                     RPFPAVMVLPVTGPSVPTGIARGIRLFAEAMGKAIVLYVKDDRTLTPELIAKLVDDGL
                     IVSVKYAVVRADPTQDDFLRAILDKVDRSIVASGIGERPAIAHMRHFGLPSFTTGSGC
                     IAPRASLQLLSLLKNKDYETAEKVRQAFLPLEDLRDAINPIRVLHDAVTAAGIADTGP
                     MLPGMDNLEPADRARVAEAAQALFAYDQQVCRQAA"
     gene            complement(3863..4918)
                     /locus_tag="VF226_21085"
     CDS             complement(3863..4918)
                     /locus_tag="VF226_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX7076541.1"
                     /translation="MSYIQKPSAYHPALKRNALGLTRRDYEGAMSTLCAGCGHDSITA
                     AIIQACFELDLPAHRIAKVSGIGCSSKAPTYFLGRSHGFNSVHGRMPSITTGANLANR
                     DLVYIGVSGDGDTASIGLGQFAHIMRRRLNMVYIVMNNGCYGLTKGQFSATNDKSSPS
                     KKGEPNPFDPIDLCQMAIQLGAGFVARSFSGDKRQLVPLIQAAISYRGFAFLDVISPC
                     VTFNNHSASTKSYDYVRAHNRAVDKLDFVPDEAEITVDYEEGTTTSVTLHDGSRLMLH
                     KLDQTHDPRDADAALMTIRKRAAQGELATGLLYVNEEQYDLHDVLRTSEKPLNAIPMS
                     DLCPGSAKLDSINARFR"
     gene            complement(5067..5369)
                     /locus_tag="VF226_21090"
     CDS             complement(5067..5369)
                     /locus_tag="VF226_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076542.1"
                     /translation="MAHVVKRKMVEVRKRGFFGWIFLIVFWVANGLMALWLFATLANW
                     GEMATPKSEAEQVGAGLGMVVGLGVIFSIWLCVAGVTGLLAFMTRGRKEIVEMEID"
     gene            complement(5380..7185)
                     /locus_tag="VF226_21095"
     CDS             complement(5380..7185)
                     /locus_tag="VF226_21095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX7076543.1"
                     /translation="MSGVNDFVCKFATVNGSGSASANTMFAKTIFRMGVPVSPKNIFP
                     SNIQGLPTWYEVRVSEKGYLARRDGIDLMVAMNPQTYKEDLAEIVPGGWLLYDSTMPR
                     NWPREDIHVLPIPIAAMCAEKWTDPRQRQLFKNMVYVGALTALLDMDMEIVKGVIVDQ
                     LRSKEKLVSANLDAVALGRDYALANFECPLPIRVRKADGARGKIMVTGNNAAALGAIY
                     AGATVCSWYPITPSTSVAEAFESYLKRLRTDKDGKVLGAVVQAEDELAAIGIAIGAGW
                     NGARSFTATSGPGISLMSEFLGLAYFAEIPVVLFNIQRGGPSTGMPTRTQQSDIIACA
                     YASHGDTKHVLLFPSDPNECFTLGATAFDLAERLQTPVMVLSDLDIGMNDWMTEPFAW
                     DDSHQHDRGKVLDAAGLDAAKDWGRYRDVDGDGIPYRTLPGTHPSKGAFFTRGTSHDP
                     YARYTEDGRINAENMLRIERKFKTAAALVPKPEILTRDPRSRVGVVYFGSTRPAVLEA
                     LDELEAEGFRINAMRIKAFPFPPEVKAFCDAHDRIFVVEQNRDAQMRSLLILEAGVDG
                     EKLVAATNFDGMPLTADFVRKSIRTSLGPIKVAAE"
     gene            complement(7202..9001)
                     /locus_tag="VF226_21100"
     CDS             complement(7202..9001)
                     /locus_tag="VF226_21100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7076544.1"
                     /translation="MAPTDVSNPQYFHKVVDCQWACPSHTPVPEYIRLIAQGRYAAAY
                     MLNWESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADYKGEIDAFLPEI
                     PKEKNGKRIALLGAGPASLTVARDLLPLGYHCVLFEKDLNGGGLMRTNIPSFRLPANV
                     LDEEVNRILDFGVETRFGHEVTSLKAVLDEGFDAVFVGTGAPKGKDLNIPGRKEGAAN
                     IHIGIDFLTSVAFEHINSIGRRVVVIGGGNTAMDCCRTALRLGGEDVKVTVRSPRKDM
                     KASDWEIEDAEREGVPILDNHNPKEFIIEGGRLAGVRFEIMRAEYTDGRRKLIPTGEE
                     VVIPCDDVLMAIGQENAFPWIESDLGLEFDQWHMPKVDRMTMMSTRPGVFFGGDAAWG
                     PENIIWAVAHGHQAAISIDAYCQGRPLTERPPPGWTLVSQKMGIHEWSYSNAYVPERR
                     VAVPHADKATALRNLKVEVELGYDDKLAAKEVERCLNCDVQTVFTRSLCIECDACMDI
                     CPVDCINFIENAEEPELRESLRVPARNTAQDLYVSDVLKTGRVMVKDEDLCVHCGLCA
                     ERCPTGAWDMQKFFYVEAQASSACLAHHNAYIR"
     gene            complement(9332..9988)
                     /locus_tag="VF226_21105"
     CDS             complement(9332..9988)
                     /locus_tag="VF226_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEX7076545.1"
                     /translation="MSVSLSEIREKLFTSVLSDCLDAVGVMNQALPSRIRPLDDNLVM
                     AGRARTAAFMEVYHQDAGGNPYELEIQLVDSLEPGDIPVFACSNPNRVAPWGELLSTA
                     AQVRGAAGALMDGCTRDVKAIRAMGFPVFHCGIAPLDSKGRARIMALDVPIECAGVRV
                     EPGDLVFGDADGVVVIPKRVEAEVLELAFEKVKGESSTLEALRAGEKLADVFARHGIL
                     "
     gene            complement(10029..11024)
                     /locus_tag="VF226_21110"
     CDS             complement(10029..11024)
                     /locus_tag="VF226_21110"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076546.1"
                     /translation="MTFRVGVFSDLRDSRGEPIFGRKPLEVLDQPGIEWEWVDGGYPE
                     ITPDIAAKYDALHINLQRVTEASVSGPDRRVKIIARNGVGYDTVDVAACTRKGVLVTN
                     TPLAVRRPVAVAALTLVFALAGRLFEKDELVRTDRWNDRNDFMGYGLMTRTLGLVGAG
                     SIGQEILRLARPFFGRMIAADPYVDAGTISKLGAELLPFDDVMRQSDFVVVCCLLTPE
                     TQKLIDARALSLMKPTAFFVNIGRGPIHDEAALVAALREGRIAGAGLDVTELEPLPAD
                     SPLIGLKNTILTPHALCWTDECFEDIATTALTSIVDALNGRRPKHLVDQAAWKNG"
     gene            complement(11021..>11783)
                     /locus_tag="VF226_21115"
     CDS             complement(11021..>11783)
                     /locus_tag="VF226_21115"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX7076547.1"
                     /translation="TLRQRAMQGDQVLGAMVFEFFSPGIAQILKLAGCEFVLYDMEHT
                     GMSLETLKVQVAACRGIGVAPMVRVPRGEYHFLARALDVGAQGLMIPMVESEAQAKAI
                     VEATHYPPKGRRGAAFGFPHDDYAPGNPLDKIRIADDRNLVIAQIETERGLEAVDEIA
                     AVDGIDVLWVGHFDLTNFLGIPGQFDHPKFLEAIKQVVAAARKHDKGLGFMPTDERWA
                     QEFKGHGFNMLAAGTDQAILLEGYRKILQAVGGKA"
BASE COUNT         2245 a         3711 c         3652 g         2175 t
ORIGIN      
        1 gtagccgtgc agtcccgcgg caagcaaaac gcacccgatc gacgcagtca ccgtcgacca
       61 gagaatggtc ggccagtcgc cgatcatcaa caaagccggc tcgtaaacga acatgaacgg
      121 cacgatgaag ccggcggcgc caatcttcac ggcggctatt ccgctggccc aaagctctga
      181 ttttgcgagg ccagcggcag cgaacaccgc cagcgcgacg ggcggcgtga tcgctgacag
      241 aattgcgaaa tagaaagcga acatatgggc agccggctcg atcacaccga gcttgatgat
      301 cgccggcacc agcagcgagg tcatgatgat gtaggctggc gtcgtcggca tccccatgcc
      361 gagaacgatc cctgcgatca tcgtcagcac caacgcaaga agtagcgttt cctgcgcgag
      421 gccgacgacg acctgcgtga agacgatgcc caagccagtc agtgtcacca cgccaatgat
      481 gatgccggcc gtggcgcagg ccagcgcgat gccgacagca ttgcgggagc cctcgatcat
      541 cgcttcgatg atgatcccga gacgcacgtt gccgcgcgtc gatgctcgaa ggaatgcgac
      601 tgggaagcag gcaagtgtac cggccagcgc tgcgagtggc gcgctgtagc ccgagtacat
      661 gacggccaag atcgtcagca cgggtatgaa caggtgcccg cgttccgcca tcacgcgcgg
      721 gagcgacggc agctcggagc gcggcagacc cattatgccg tggcgcttgg cctcgaaatg
      781 caccgccgag aagcaggcga gatagtagag aagcgccgga atgaacgccc agatgatgac
      841 ctggccgtag ccaacctgca ggaactcggc catgacgaaa gcggccgcac ccatgatcgg
      901 aggcatgatc tgcccgccgg tggacgccac tgcctcgact ccggcggcaa agggcggtcg
      961 gaacccggta cgcttcatca gtgggatcgc aatcggcccg tcaaccatga cgttggccac
     1021 cgccgagccc gagatcgttc cgaacaggct cgacgagaag acggaaactt tgccgggccc
     1081 accggccgtg tggccagtca ggctcatggc gaagtccata aagagctgcc cggtgccggt
     1141 ccgctccatg aacgcgccga acatgacgaa aatcaaaacg tatgaggcag acactgccag
     1201 cgggatgccg aaaattccct ctgtcgtcat gtagagctga tcgagcagcc gcatcggctc
     1261 aacctgcgcg accagcagtc cataggcgag aaacacgagt gccgtcagcg gcagtgcagc
     1321 gccgagcgcg cggcgcgtcg cctcgaggat catcagagtg agaacggtcc cgagcaccat
     1381 atcgagagtg ctcaggtcgt cgacgtagta gatgcggttg acgacgtaat cgtattcaac
     1441 gaacagatag aagatcggca ctatggacag caccaggaac gcgtagtcga gaagcgtcgg
     1501 tgtctgacca gcctgggccg actttctacg gtagaccagg aagcacagcg acatcgcgaa
     1561 gaagaggtga gtgccgcgat agaggatcgc ctccggcgca cccacccaga tgtgccacat
     1621 atggtagagc gccatcgaga cgcccagaac gccaacggcg agacgcacga tgccggcgtg
     1681 agtgaattcg aatttcatat gtcgtccaaa agataggtgg gtctgcccgc gacctcatcg
     1741 ggcaaatggg gccgcctcct cggcggcccc gaatgcgggt cacttcacct cgacgccctg
     1801 ctccttatag aacttggcgg cgcccgggtg gaacggcacg ccgacgtcct gcgccatctg
     1861 cttcggtgtc aggctggcca tggccttgct cgtggccgcc agtgatggga cgttttcggc
     1921 gatacccttg gtcatctggt agaccgtttc ctcaggcagg ctgcaggcgg cgacgatgtg
     1981 cgtggcatag ccgatcacat tcaccgcctt gtcctgcttc ggataggtgc cggccgggac
     2041 ctcgatcaat gtatatcccg gattcagctt cttcataccg tcgatggcat cggagagatc
     2101 gagcagcttg atgtcacgcg ccgcggcgat gtccatgatg gcggaggccg gaatgagggt
     2161 gcccagcgtg aacatctgcg cctggttgtc cttaagctgc gtcgccgagt ccgtgtagga
     2221 cacgaagctc gtcttcatgt ccgagtagct gaggccgtac accttcagaa gctgctgggt
     2281 gatcatctct gcagtgttgc ccctgggctg cgtcgtcagt gccttgccct tgatgtcctt
     2341 cacggagttg atgccagcgt ctgccggcac gacgacctgg aagtactgcg ggtagagcgt
     2401 ggcaacgttg caaaccttct tatgcggctc cttgaacggc ggattgcccc tgatcgcgtc
     2461 ggcggtcgtg atggtgttgc cgaaaccgac ctcggccttg tcagtctcga tggcccgcac
     2521 gttggcgaca ccggcgccag ggagcgactg gacctgcagg cccggaatcg ccttctccca
     2581 gatatccttg agctgtccac caagcgggat ccagctgccg ccctgcgggc cggtcatgag
     2641 acggatctgt tccgcctgag ccgccccgct tgtaagagcg aatgccgcac ccgcggcaag
     2701 cagcgcatac ttcagtttgt ccaacatcct gggcttcctt tcatagtcgt tctggttggc
     2761 tggaaaatgt cgcggccgtc tcaagcggcc tggcgacaaa cctgctggtc atacgcgaag
     2821 agcgcctgcg ccgcttcagc gacgcgcgct cggtccgccg gctcgaggtt gtccattcca
     2881 ggcagcatcg gacccgtatc ggcgatgccg gctgcagtga ccgcgtcgtg cagcacccgg
     2941 atgggattga ttgcatcgcg cagatcctcc agcgggagaa aggcctggcg gactttctcg
     3001 gcggtttcgt aatccttgtt cttcagcaga gagaggagct gcagggaagc tcgcggcgca
     3061 atgcatcccg agcctgtcgt gaacgagggc agaccgaaat ggcgcatatg cgcgatggcc
     3121 gggcgctcac cgatgccgct ggcgacaatc gagcggtcga ccttgtcgag gatagcccgg
     3181 aggaaatcgt cctgggtggg gtcggcgcga acgacggcgt acttcacgga tacgatgagt
     3241 ccgtcatcga cgagtttcgc gatgagctcc ggagtgagcg tccgatcatc cttcacgtaa
     3301 aggacgatgg ccttgcccat ggcttcggcg aatagacgga tcccacgcgc aattccagtc
     3361 ggaacggatg gtccggtcac cggtagcacc ataacagcgg ggaacggccg cgacttcaga
     3421 attgcagcct ggtcccgcag cttgccgaag tccggtccgg cggacgggag cagccacgta
     3481 tccggcccga cgctttcggc cagaaaatca agaatttcgg catactcgcc gatgctgata
     3541 ttgaagaaaa cggcgttgcc cccatacatg agggaacgaa taccggccga ctccagatgt
     3601 ttgatgatgg caagattcgc cggccggttc agccgcaact cgctatcacg agcaagcggc
     3661 ggaacagcga tgaccgagcg cgcgatatcc gccggcgtga cctgcgtcgt cttcatcctg
     3721 agatcctccg atgcgccccg atagctgccc ctgcggcgcc tgtcgattaa tgcagcaacg
     3781 gtttaggaac tcaggctcaa agtcaagtat cgaccgtcca gatattgcga aacgaaacgg
     3841 gccgattacc gagacctctg gatcagcgga acctggcatt gatgctgtcg agcttggccg
     3901 agcccgggca gaggtccgac atcgggatgg cgttgagagg cttctccgat gtgcgcagga
     3961 cgtcgtgcaa atcgtactgc tcttcattga catagagcag gccggtcgcc agctcgccct
     4021 gtgcggcgcg cttgcgtatg gtcatcagcg cggcgtcggc atcacgcggg tcgtgcgtct
     4081 ggtcgagctt gtgcagcatg aggcgcgagc cgtcgtgcag ggtgaccgag gtcgtcgtgc
     4141 cctcttcgta atccaccgtt atctcggcct catcggggac gaagtcgagc ttgtcgacgg
     4201 cgcgattatg ggcccgcacg taatcgtagc tcttggtcga cgccgaatgg ttgttgaagg
     4261 tcacgcaggg gctgatcacg tcgagaaatg cgaagccccg atagctgatc gcggcctgga
     4321 tcagcgggac gagctggcgc ttgtcgccgg agaacgagcg ggcgacgaac ccggcgccca
     4381 gttggatcgc catctggcag aggtcaatcg gatcgaaggg attgggctcc cccttcttcg
     4441 atggcgatga tttgtcgttg gtggctgaga actggccctt ggtcaaacca tagcagccgt
     4501 tgttcatgac gatgtaaacc atgttgagcc gccgccgcat gatatgggcg aactgcccga
     4561 gcccgatcga ggccgtgtca ccgtcacccg agacgccgat atagacgaga tcccggttgg
     4621 cgaggttggc ccccgtcgtg atcgagggca tgcggccatg cacgctgttg aagccgtggc
     4681 tgcgccccag gaagtaggtc ggcgccttcg aggagcagcc gatgcctgag accttggcga
     4741 tcctgtgcgc cggcagatcg agctcgaagc atgcctggat gatggccgcc gtgatcgagt
     4801 cgtgtccgca cccggcgcac aacgtcgaca tcgccccttc gtaatcgcgg cgtgtcaggc
     4861 cgagggcgtt ccttttgagc gccgggtgat aggcgctggg cttttgaatg tagctcatct
     4921 cgaatgcatc actttctgaa cgtgaggttc ctgagcgctg tggccgatct cagctcttcc
     4981 tgaatgcaat ccggcaaact cggttggact aaggtaaagc cactcgccac cagtgatcaa
     5041 aagcaaatgc ccccaatcta aaaagcttaa tctatctcca tctccacgat ttccttgcga
     5101 cctcgagtca tgaatgcgag caggccagta acacctgcca cacatagcca aatggagaaa
     5161 attaccccga ggccaactac cattcctagg ccagcaccaa cttgttccgc ttcgcttttg
     5221 ggggtcgcca tttcgcccca gttagccaac gtggcaaaca accagagcgc cattaagcca
     5281 tttgcgaccc aaaatactat gaggaatatc cagccaaaaa atcccctctt cctaacctct
     5341 accattttgc gcttaacaac atgtgccatt gcgccctcct cattccgccg ccactttgat
     5401 cggacccaag ctggtccgga tcgacttgcg gacgaagtcc gcggtcaagg gcatgccgtc
     5461 gaaattggtt gcagcaacga gcttctcgcc gtcgaccccc gcctcgagga tcagaagcga
     5521 gcgcatctgt gcatcgcggt tctgctcgac gacgaagatg cggtcatgcg catcgcagaa
     5581 ggccttcacc tcgggcggga acgggaaggc cttgattcgc atcgcgttga tacgaaagcc
     5641 ctcggcttcg agctcgtcga gcgcttccag caccgccggc cgtgtcgagc cgaaatagac
     5701 cacgccgacg cggctgcgcg gatcgcgcgt gaggatttcg ggcttgggca ccagcgccgc
     5761 cgccgttttg aacttccgtt cgatacggag catgttctcc gcattgatgc ggccgtcctc
     5821 cgtatagcgt gcataaggat cgtgcgacgt accgcgggtg aagaaggcgc ctttcgatgg
     5881 atgcgtgcca ggcagcgtgc ggtagggaat gccgtcgcca tcgacatccc gatagcggcc
     5941 ccagtccttg gcagcatcga gacccgccgc gtcgagcacc ttgccgcgat cgtgctggtg
     6001 ggagtcgtcc caagcgaatg gctcggtcat ccagtcgttc atgccgatgt cgaggtcgct
     6061 cagcaccatg accggtgtct gcagccgttc ggccagatca aacgcggtgg cgccgagggt
     6121 gaagcactcg ttgggatcgg aagggaacag caggacgtgc ttggtgtcgc cgtgggacgc
     6181 gtaggcgcag gcgatgatat cggactgctg ggtccgcgtc ggcatgccgg ttgaggggcc
     6241 gccgcgctgg atgttgaaca gcaccaccgg gatctcggcg aaataggcaa gacccaggaa
     6301 ctcgctcatg agcgaaatgc ccggcccgga ggtggctgtg aatgagcgcg cgccattcca
     6361 gccggcgccg atcgcgatgc cgatcgcagc caattcgtcc tccgcctgga cgacggcgcc
     6421 aagcaccttg ccgtccttgt cggtgcgcag ccgcttcaag tagctctcga aggcttccgc
     6481 taccgatgtc gagggcgtga tcggatacca cgagcagacc gtcgcaccgg cgtagatggc
     6541 cccgagcgcc gccgcattgt tgcccgtcac catgatcttg ccgcgcgcgc cgtccgcctt
     6601 ccgaacacgg atcgggagcg ggcattcgaa gttcgcgagc gcgtaatccc gcccgagcgc
     6661 caccgcgtcg aggttggcgg acacgagctt ctccttgctg cggagctggt ccacgatgac
     6721 gcccttgacg atctccatgt ccatgtcgag cagcgcggtc agggcgccga cgtagaccat
     6781 gttcttgaac agctgccgct ggcgcgggtc cgtccatttc tccgcgcaca tggccgcgat
     6841 cgggatcggc agcacatgga tgtcctcgcg cggccaattg cgcggcatgg tggaatcgta
     6901 gagcagccac ccgccgggga cgatctccgc caagtcctcc ttgtaggtct gcgggttcat
     6961 cgcaaccatc agatcgatgc cgtcgcggcg ggcgaggtag cccttctcgc tgacccgcac
     7021 ctcgtaccag gtcggcaggc cttggatgtt cgacggaaag atattcttcg gactgaccgg
     7081 cacgcccatt cggaagatgg tcttggcgaa catcgtgttg gcgctcgccg agccggagcc
     7141 gttcacggtc gcgaacttgc agacgaaatc attaaccccg ctcatggctc aagccctgcc
     7201 atcatcgaat gtaagcgttg tgatgagcga ggcaggcgga tgaggcctgc gcttccacgt
     7261 aaaagaattt ctgcatatcc catgctcccg tcgggcagcg ctcggcgcat agcccacaat
     7321 gaacgcagag gtcctcgtcc ttgaccatta cgcggccagt cttgaggacg tctgaaacgt
     7381 aaagatcctg tgccgtattc cgagccggca cccgcaggct ttccctgagc tccggctcct
     7441 cggcgttctc gatgaagttg atgcagtcga ccgggcagat gtccatgcag gcatcgcact
     7501 cgatgcacag cgagcgcgtg aagacggtct gcacgtcgca attgaggcag cgctcgacct
     7561 ccttggcggc gagcttgtcg tcgtagccga gctcgacctc gaccttcagg ttgcgcagcg
     7621 ccgtggcctt gtccgcatga ggaacggcca cacgccgctc cgggacatag gcgttcgagt
     7681 acgaccactc gtgaatgccc atcttctggc tgacgagggt ccagccgggc ggtggccgct
     7741 cggtgagcgg tcggccctgg cagtaggcat cgatcgagat cgccgcctga tggccgtggg
     7801 ccacagccca gatgatgttc tccggacccc acgccgcgtc gccgccgaaa aagacaccag
     7861 gacgggtcga catcatcgtc atgcggtcga ccttcggcat gtgccactgg tcgaactcga
     7921 gcccgagatc ggactcgatc caggggaaag cgttctcctg gccgatggcc atcagaacgt
     7981 cgtcgcaggg gatcacgacc tcctccccgg tcgggatcag cttgcgccgg ccgtccgtat
     8041 actcggcgcg catgatctcg aagcgcacgc cggcgagccg gccgccctcg atgatgaact
     8101 ccttcgggtt gtggttgtcc aggatcggaa cgccttcgcg ctcagcgtcc tcgatctccc
     8161 agtccgacgc cttcatgtcc ttgcggggag agcggaccgt caccttgacg tcctcgccgc
     8221 cgagcctgag ggccgtacgg cagcagtcca tggccgtgtt gccgccgcca atgaccacga
     8281 cccgccggcc gatgctgttg atgtgctcga aggcgaccga ggtcaggaag tcgatgccga
     8341 tgtggatatt ggcggcgcct tccttacggc cagggatgtt gaggtccttc cccttggggg
     8401 ctccggtgcc gacgaatacg gcgtcgaacc cctcgtcgag aacggccttg aggctcgtca
     8461 cctcgtgacc gaagcgcgtc tcgaccccga agtcgagaat gcggttcact tcctcgtcga
     8521 gaacattcgc cggcaagcgg aaggatggga tgttcgtccg catcaggccg ccgccgttca
     8581 ggtccttctc gaacagcacg caatggtatc cgagaggcag caggtcccgt gcgaccgtca
     8641 gcgaggcggg gccagccccg agaagcgcaa tacgtttgcc gttcttctcc ttgggaattt
     8701 ccggaaggaa ggcatcgatt tcgcccttgt agtcggcggc cacgcgcttc agccggcaga
     8761 tcgccaccgg cttctcctcg acgcgaccgc ggcggcaggc cggctcgcat gggcggtcac
     8821 agacgcgccc cagaatgccc ggaaagacgt tcgattccca attcaacata taggccgctg
     8881 cgtagcggcc ttgtgcgatc aaccggatgt actctggaac aggtgtatgg ctggggcacg
     8941 cccactgaca gtctacgact ttgtggaaat actgcggatt ggaaacatcc gtcggagcca
     9001 tgccccggct ccgagtgcgc acagcggcgt tgccttgcgg cataggaact ccattctcca
     9061 gtcgttcgaa cccagagcca tgcccatggg tgcggccccg attggtacag gctcaactta
     9121 gtgacccgag cctaccgatg gcaagctctg gcaggcatga cagccatcaa aattaggatg
     9181 atttgtcccc tcaaatgggt gtcatcagtc gtcagagcgt atgggcgtgt tcgcctatat
     9241 tgcatccgcg cggtttttgc agttttcagc ctggccattt cgctcttgca gcaaaattcg
     9301 tacccgctcg cttatgtgcg gcgcaccata atcagagaat accatgccgc gcgaacacat
     9361 cggccagctt ctcaccggct cgtaaggctt cgagcgtgct gctttcccct ttgaccttct
     9421 cgaacgcaag ttccagcact tccgcctcca cgcgcttcgg gatcaccacc acgccgtccg
     9481 catcgccgaa gacgagatcg ccgggctcga cgcgtacacc ggcgcattcg atcggcacat
     9541 ccagggccat gatccttgcc cgccccttgc tgtcgagcgg agcgatgcca caatggaaga
     9601 ctggaaagcc catcgcccgg atcgccttca cgtcgcgcgt gcagccgtcc atcaaggcgc
     9661 cagccgcccc acggacctgc gcagcggtcg aaagcagctc gccccacggc gcgacgcggt
     9721 tgggattgga gcaggcaaac actgggatgt cgcccggctc caggctgtcg acgagctgga
     9781 tctcgagctc atatggattg cctccggcgt cctggtggta gacctccatg aacgccgccg
     9841 tgcgggcccg cccggccatg acgagattgt cgtcgagcgg ccgaatgcgg gaagggagcg
     9901 cctggttcat gacgcccacc gcatcgagac agtcggagag cacggaggtg aacagcttct
     9961 cgcggatttc gctcaatgat accgacattg cgccgttgcc tcctcagcca acatgagggc
    10021 catttcgatc atccgttctt ccaggccgct tgatcgacca gatgcttggg ccgcctgcca
    10081 ttgagcgcat cgacgatcga cgtcaacgcc gtcgtcgcga tgtcctcgaa gcactcatcc
    10141 gtccagcaga gtgcgtgcgg ggtgaggatg gtgttcttca gcccgatcag agggctgtcc
    10201 gcgggcagcg gctccagctc ggttacgtcg agtcccgccc cggcgatcct gccctcacgc
    10261 aaggcggcca ccagcgctgc ctcgtcgtgg atcggaccgc ggccaatgtt cacgaagaag
    10321 gcggtcggct tcatcaggct cagcgcacgc gcatcgatca gcttttgggt ctcgggcgtc
    10381 agcaggcagc agacaaccac gaagtcggac tggcgcataa cgtcgtcgaa cggcagcagc
    10441 tccgcaccga gcttggagat cgttccggca tcaacatacg ggtcggccgc gatcatccgg
    10501 ccgaagaacg gcctcgccag ccgcaggatc tcttggccga tgctgcccgc cccgacgagt
    10561 cccagcgtgc gcgtcatcag gccgtagccc atgaagtcgt tacgatcgtt ccagcggtcg
    10621 gtgcggacga gctcatcctt ctcgaacagc cggccggcca gggcgaacac gagcgtgagc
    10681 gcggcgaccg caacggggcg gcgtacggcc agcggcgtat tggtgacgag cacgcccttg
    10741 cgggtgcagg ctgccacgtc gacggtgtcg taacccacgc cgttccgcgc aatgatcttc
    10801 acccggcgat ccggaccgga aacggaggcc tccgtcacgc gctgcaagtt gatgtgtagc
    10861 gcatcgtact tcgcggcgat gtccggggtg atctcgggat agccgccgtc gacccactcc
    10921 cactcgattc cgggctgatc caggacctcg agcggcttgc gtccgaagat cggctccccg
    10981 cgggaatcgc gcaggtcgct gaaaacgccg accctgaacg tcatgccttg cccccgaccg
    11041 cctgcaggat cttcctgtag ccttccagca gaatagcttg gtctgtgccg gcggccagca
    11101 tgttgaagcc gtgccccttg aactcctggg cccagcgctc gtcggtcggc atgaagccga
    11161 ggcccttgtc gtgcttgcgc gcggcagcga cgacctgctt gatggcctcc aggaacttcg
    11221 gatgatcgaa ctggccgggg atgccgagga agttcgtcag atcgaagtgg ccgacccaga
    11281 gcacgtcgat cccgtccacc gccgcgatct cgtccacggc ttcaaggccc cgttccgtct
    11341 cgatctgggc gatgacgaga ttgcgatcgt cggcaatcct gatcttgtcg agtggattgc
    11401 ccggcgcata gtcgtcatgc gggaagccga atgcggcccc gcgccgtcct ttgggcggat
    11461 agtgcgtcgc ctcgacgatg gccttggctt gcgcctcgct ctcgaccatc gggatcatca
    11521 gcccttgcgc cccgacatcg agcgcccgcg ccagaaagtg gtactcgccg cgcggcacgc
    11581 ggaccatcgg agcgacgccg atgccgcggc aggccgcaac ctggaccttg agcgtctcga
    11641 ggctcatgcc ggtgtgctcc atgtcgtaga gcacgaactc gcagccggcg agcttcagga
    11701 tctgggcgat gcccggcgag aagaactcga acaccatggc gcccagcacc tggtcgccct
    11761 gcatggcccg ctgcctcagc gtt
//
LOCUS       DASVDU010000316         3874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1254665, whole genome shotgun sequence.
ACCESSION   DASVDU010000316 DASVDU010000000
VERSION     DASVDU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254665"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF226_21120"
     CDS             <1..281
                     /locus_tag="VF226_21120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7076548.1"
                     /translation="AQQGLKDPEEAGAAASDYLRFFALVALGWMWLRMARVATDRLAA
                     GTDEPEFYQAKLAVAKFYMARILPQHTALAVAMTSGKAPIMELPEAAY"
     gene            complement(455..610)
                     /locus_tag="VF226_21125"
     CDS             complement(455..610)
                     /locus_tag="VF226_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076549.1"
                     /translation="MALLLLKKGWFVVVAVLAGGWAAVKRFFGRRPVAAPLAPERTDG
                     PDSGGAG"
     gene            786..1734
                     /locus_tag="VF226_21130"
     CDS             join(786..1121,1123..1734)
                     /locus_tag="VF226_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612137.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7076550.1"
                     /translation="MARPLSIDLRERVVAAVLAGESCRSVAARFGVSVSSAVKWSQRH
                     RATGSVAPGKMGGHRKPVLEPHRDFILEQIARTPHLTLHRLKDELAAHGVSVSHTAVW
                     TFLRREGLRFKKTLLAIEQARADVARRRQRWRSWQPRLDPRRLVFIDETWIKTNMAPL
                     RGWGPKGKRLRGFTPHGHWRTLTFLAALRCDRLTAPCVFDGPINGQCFRAYVEQQLVP
                     VLEPGDIVVMDNLGSHKAAAVRQMIQAAGARLWYLPPYSPDLNPIEQTFAKIKHWMRL
                     AQERTIDDTCRHLGRLVPTIEPAECASYFTNAGYASVKT"
     gene            complement(1741..2517)
                     /locus_tag="VF226_21135"
     CDS             complement(1741..2517)
                     /locus_tag="VF226_21135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX7076551.1"
                     /translation="MAQSAAQPGMQVSDEVMIEVSGLHKWYGQFHVLKNIDLTVYKGE
                     RIVICGPSGSGKSTLIRCINRLEEHQRGRIVVDGTELSNDLKHIEQIRREVGMVFQHF
                     NLFPHLTVLQNLTLAPIWVRKMPKAEAERTAMHYLERVKIPEQAGKYPGQLSGGQQQR
                     VAIARSLCMNPKVMLFDEPTSALDPEMIKEVLDVMIELAESGMTMLCVTHEMGFARTV
                     ANRVIFMDGGEIIEQNDPQGFFNNPKSERTKLFLSQILHH"
     gene            complement(2530..3651)
                     /locus_tag="VF226_21140"
     CDS             complement(2530..3651)
                     /locus_tag="VF226_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7076552.1"
                     /translation="MTDETLTTPERYEPGRHPDLPPPASTVGVLGWLRTNLFSSPLNT
                     ALTIGSLYLIVPPVLDWAIFSANWNDGTSRADCVKDGACWTMVRARFGQFMYGRFPID
                     ERWRVDLAFILFLVTAVPLLFDRTPGKKYLAGFVFFVLPVIAYFLFYGGLGLRIVDTS
                     LWGGLMLTLILGIVGIVFSLPLGIMLAIGRRSDLPIIRTLCIAFIEFVRAVPLITVLF
                     MASVMLPLFLPAGVNFDKLLRAMIGIALFGAAYMAEVVRGGLQAIPRGQYEGAMALGL
                     GYWQMMGLIILPQALRISIPGIVNTFIGLFKDTTLVIVIGLFELLGTVQQATRDANWA
                     GLSNEGFVFAAVIYFIFCFSMSRYSVRLERKLHTGHKRT"
     gene            complement(3653..>3874)
                     /locus_tag="VF226_21145"
     CDS             complement(3653..>3874)
                     /locus_tag="VF226_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX7076553.1"
                     /translation="PLTSQYLNLTKNSSLAVVIGYPDLVSVFAGTVLNQTGQAVEVIA
                     ITMAVYMTISLIISLFMNWYNRRVRLVER"
BASE COUNT          707 a         1270 c         1236 g          661 t
ORIGIN      
        1 tcgcccagca gggactcaag gatccggagg aagcgggcgc cgcggcgagc gattacctgc
       61 gcttcttcgc gctggtcgcg ctcggctgga tgtggctgcg catggcgcgc gtcgcgacag
      121 acaggctcgc cgccggcacc gacgagcccg agttctacca ggccaagctc gccgtcgcga
      181 aattctacat ggcgcggatc ctgccgcagc acaccgcgct cgccgtggcg atgaccagcg
      241 gcaaggcccc gatcatggag ctgcccgagg cggcgtattg aaccacaccg ttaaacgcca
      301 acaatgcttc tgcttcctca ttgtcatgcg ggctcgaccc gcgcatccat tcatccgccc
      361 gcgcaagcgg ctggatgctc gccgggccga gcccggcgat gacaatcggc gatagccaga
      421 cggcaccttg acgccgctcg gcttcggact cgcatcaacc ggctccgccg ctgtctggtc
      481 cgtcggtccg ttcgggcgcg agcggcgcgg ccaccggacg gcgcccgaag aatcgcttca
      541 ccgctgccca gccgccggcg agaacggcga cgaccacgaa ccaccccttc ttcaggagaa
      601 gtaacgccac cgcgaccagg ccggccagca ggcccttggc tttctcgccg ccagccctga
      661 ccgcgactag cgaggcgatg ccgaagcccg ctagagcgtt tcatggctta gctgaatcgg
      721 aatgggattc cggtttggct gaatttgtga ttcaagatgc tggctggagg ggaggccagc
      781 atcgaatggc tcgacctttg tcgatagatc ttcgtgagcg tgtggttgcg gcggttctgg
      841 ccggagagag ctgccggtct gttgcggctc ggttcggtgt ttccgtctcc tcggctgtga
      901 aatggtcgca gcgtcatcgt gcgaccggct cggtagcgcc gggcaagatg ggcgggcatc
      961 gcaagccggt gctcgagccg caccgcgact tcatcctgga gcagattgct cggacgcctc
     1021 acctgacgct gcaccggctg aaggacgagc tggccgccca cggcgtgagc gtctcgcaca
     1081 ctgcggtatg gacgttcctg cgacgcgagg ggcttcggtt caaaaaaaac cctgctcgcc
     1141 attgagcagg cccgcgccga cgtcgcgcgc cggcgtcagc gctggcgatc ctggcagccc
     1201 cggctcgacc cgcggcggct ggtgttcatc gacgagacct ggatcaagac caacatggcg
     1261 ccgctgcgcg gatgggggcc gaagggcaag cgcctgcgcg gctttacccc gcatggccac
     1321 tggcgcacgc tgaccttcct cgccgcgctg cgctgcgacc ggctcaccgc cccttgtgtc
     1381 ttcgacgggc cgatcaatgg gcaatgcttc cgagcctatg tcgagcagca gctcgtgccg
     1441 gtgctggagc cgggcgacat cgttgtcatg gataatctcg gctcccacaa ggcagccgcc
     1501 gtccgacaga tgatccaggc cgccggcgcc aggctctggt acctgccgcc ttactccccc
     1561 gacctcaatc cgatcgagca gacctttgcc aagatcaagc actggatgcg gctggcccag
     1621 gagcggacca tcgacgacac ctgccgccac ctcggacgcc tcgtcccaac aatcgagccg
     1681 gccgagtgcg ccagctactt caccaacgcc ggctacgctt ccgtcaaaac atgaaacgct
     1741 ctagtggtgc aggatctggc tcaggaagag cttggtccgc tccgacttcg gattgttgaa
     1801 gaacccctgc gggtcgttct gctcgatgat ctcgccgccg tccatgaaga tcacccggtt
     1861 ggcgaccgtc cgggcgaagc ccatctcgtg cgtgacgcag agcatggtca tgccgctctc
     1921 ggccagctcg atcatcacgt cgagcacctc cttgatcatc tccgggtcga gcgccgaggt
     1981 cggctcgtcg aacagcatga ccttcgggtt catgcagagc gagcgggcga tggccacacg
     2041 ctgctgctgg ccgccggaca gctggccagg atattttccg gcctgttccg ggatcttcac
     2101 ccgctccagg tagtgcatgg cggtgcgctc ggcctccgcc ttcggcatct tgcgcaccca
     2161 gatcggggcc agggtcaggt tctgcagcac ggtcagatgc gggaacaggt tgaagtgctg
     2221 gaacaccatc ccgacctcgc gccggatctg ctcgatatgc ttgagatcgt tcgaaagctc
     2281 ggtcccgtcg acgacgatgc ggccccgctg atgctcctcc agccggttga tgcagcgaat
     2341 cagcgtcgat ttgcccgagc cggacgggcc gcagattacg atgcgttcgc ccttgtagac
     2401 cgtcaggtcg atgttcttca gaacgtggaa ctggccatac cacttgtgga ggccgctcac
     2461 ctcgatcatg acttcgtcgg aaacctgcat gccgggctgt gcggctgact gcgccatgtg
     2521 tctttctcct caggtcctct tgtggccggt atgcagcttg cgctccagcc gcacgctgta
     2581 gcgcgacatg ctgaagcaga agatgaaata gatcaccgcc gcgaacacga atccttcgtt
     2641 cgacagcccc gcccagttcg catcgcgcgt ggcctgctgc accgtgccca gcagctcgaa
     2701 caacccgatc acgatcacca gcgtggtgtc cttgaacagg ccgatgaagg tgttgacgat
     2761 cccgggaatt gagatccgca gcgcctgagg caggatgata agccccatca tctgccagta
     2821 gccgaggccg agcgccatgg cgccttcgta ctgcccccgg ggaatcgcct gcaggccgcc
     2881 gcgcaccact tcggccatat aggcggcgcc gaacagcgcg atgccgatca tcgcgcgcag
     2941 cagcttgtcg aagttcacgc cggcgggaag gaacagcggc agcatcaccg aggccatgaa
     3001 cagcacggtg atgagcggaa ccgcccggac gaactcgatg aatgcgatgc agagcgtacg
     3061 gatgatcggc agatcggatc gtcggccgat cgccagcatg atcccgagcg gcagcgagaa
     3121 cacgatgccg acgatgccga ggatgagcgt cagcatcagc ccgccccaga gcgaggtgtc
     3181 gacgatccgg aggccgaggc cgccatagaa caggaaatat gcgatcacgg gcaggacgaa
     3241 gaacacgaac ccggcgagat acttctttcc cggcgtacgg tcgaacagaa gcggcacggc
     3301 cgtcaccagg aacaagatga aggccagatc gacgcgccag cgctcgtcga tcgggaaccg
     3361 gccgtacatg aactgcccga agcgcgcgcg caccatggtc cagcaggcgc cgtccttcac
     3421 gcagtcggcg cgcgacgtgc cgtcgttcca gttcgccgag aagatcgccc agtccagcac
     3481 cgggggcacg atcagataga gggaccctat ggtgagcgcg gtgttcaacg gcgaggagaa
     3541 cagattggtc cgcagccatc cgagcacgcc gaccgtgctg gccggcggcg gcaggtccgg
     3601 atggcgtccg ggctcgtagc gttccggcgt ggtaagcgtc tcgtccgtca tgtcaccgct
     3661 ccaccagccg gacgcgccgg ttgtaccagt tcatgaacag cgagatgatc aggctgatcg
     3721 tcatgtagac cgccatggtg atggcgatga cctcgacggc ctggccggtc tggttgagga
     3781 cggtccccgc gaacacgctg accaggtcgg gatagccgat caccacggcg agggacgagt
     3841 tcttcgtgag gttgagatat tggctggtga gcgg
//
LOCUS       DASVDU010000317        10081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1271440, whole genome shotgun sequence.
ACCESSION   DASVDU010000317 DASVDU010000000
VERSION     DASVDU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271440"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VF226_21150"
     CDS             complement(<1..876)
                     /locus_tag="VF226_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7076554.1"
                     /translation="MTTLSNYAMRDVETILHPATNLALHRTTGPLVLERAEGVYVWDK
                     EGKRYIEGLAGLWCTGLGYGNQELVEAAREQMSKLSFTHLFGGRSHEPAIALAEKLKE
                     LVPAPASKIFFTSSGSEANDTQIKLAWYYNNARGLTKKKKIISRMKAYHGVTIASGSL
                     TGLPVFHADFDLPMQRVLHTDCPHYWRYAEEGESEEEYATRLASNLEELIQREDPETI
                     AAFIAEPVMGAGGVIVPPRTYFEKIQAVLRRYDILMIADEVICGFARTGNWFGSQTFG
                     IQPDTITMAKAITSAY"
     gene            990..1895
                     /locus_tag="VF226_21155"
     CDS             990..1895
                     /locus_tag="VF226_21155"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEX7076555.1"
                     /translation="MTSLDLSWHAVHRMIDATRRRPVFAAVWSARLALFSGGLAIVGI
                     LLHRLLGLPTPILLNVLKVAFAGAALALVLGLMGAVQVWFTGRTGAGAATVGLLASLL
                     LFAWPAYYYPAMRDLPAINDVTTDPDAPPPMMALAKLRGPGANPVAYPGERFAELQAA
                     AYPDLKPFLIPRPADEAFEIAAEAVRQLRYKVVSETPPGESFEEPGYIEAVERTFVMG
                     FYDDIVIRVMGDSEASQIDIRSASRYGTHDLGQNASRIRTILAELRKVMDSTVPAEAN
                     PRNARSKQKATQQKRGGRGRAGRRN"
     gene            complement(1846..2754)
                     /locus_tag="VF226_21160"
     CDS             complement(1846..2754)
                     /locus_tag="VF226_21160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7076556.1"
                     /translation="MTAEIPFNKTMEFAYGVPRELAPGIVRLVANNPSPFTFHGTNTY
                     LIGSRELALIDPGPEDEAHFEAIMRTAAGRPITHIFITHTHRDHTDGLDRIREATGAK
                     ICARGRSLRGPGSLKTSPSGAEFIDIDFKPDIVMGDGDVVAGPDWRLEALFTPGHAPD
                     HLCFAEASSNVLFCGDHVMGWNTSVVAPPEGNMTDYMRSLEKLLERSEPVFLPGHGGR
                     IEQPSRMVKAYIVHRRWREQAILDAVRQGHITIDRIVAAVYQGLDPRLVRAASLSVLA
                     HIESLVDRGLVNCDGPPDLDRPVSAA"
     gene            complement(3015..3986)
                     /locus_tag="VF226_21165"
     CDS             complement(3015..3986)
                     /locus_tag="VF226_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7076557.1"
                     /translation="MSALIEVLGLSKTFSVSRGMFARRATLNAVTGVDLDVQKGEVLG
                     IVGESGCGKSTLARMILGLLPPTTGSVRLGGKDIRTIPRREVARRMQPVFQDPYSSLN
                     PRRRIASIVALPLEVQGIGSAEERRAKAIAALDKVGLPSRYADNYPSQLSGGQRQRVA
                     IARALVTQPEIVICDEPTSALDVSVQSQILNLLMDLKQNLGLTYVFISHNLAVVEHLA
                     TRVAVMYLGRVVELAPTAELFRNPRHPYTQALLASVLTPEPGLGIPEALLGTASPDPL
                     NIPSGCPFHPRCPQAMEMCRSIVPKPVTTASGFAACHLVDNAASRAA"
     gene            complement(3983..4999)
                     /locus_tag="VF226_21170"
     CDS             complement(3983..4999)
                     /locus_tag="VF226_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076558.1"
                     /translation="MMTGVSPEPVLDVQDLSVTIRTPAGPLHAVRGVSFDVRRGETVC
                     LVGESGCGKSMTSLAIMDLLPANATRRAGRLVFAGTDLTRASRAEINALRGNRMAMIF
                     QEPMTALNPAWTIGDQLIEVYRRHKRSGFAEARDRAIDLLAKVGIASPAERLGQYPHQ
                     LSGGLRQRVMIAMALMCGPDLLIADEPTTALDVTIQAQILRLLAGLQRELGIALLLIT
                     HDLGIVARIADRVHVMYAGAIVESATTQELFARPCHPYTRGLLSCLPRSGITEAGTRL
                     GTIPGVVPSLIGGIEGCAFRSRCSYARAACAVAIPIRSPSATHRYVCVEEFGATSELG
                     EAIA"
     gene            complement(4996..5823)
                     /locus_tag="VF226_21175"
     CDS             complement(4996..5823)
                     /locus_tag="VF226_21175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076559.1"
                     /translation="MAGNPSVVTGLILVGAVVAIALLAPLLAPHDPYAQDLMRRLVPP
                     IWHDKGIWSNPLGTDNLGRDYLSRILYGARISLLIGLSVMVISGLIGTALGLAAGYFG
                     GRTDLVISFFITARLAMPVVLVALAVVAMIGGSLPVVILTLGLLKWDRFAVVMRSATQ
                     QVRSLDYVTAAEAAGASRLRILLGEVLPNVFPHLIVVATVEAGSAILLEAALSFLGLG
                     VQPPLPSWGLMIAEAKNFMFFSFWLIAIPGTALALLVLGINLLGDGLRDVFAPDERA"
     gene            complement(5889..6806)
                     /locus_tag="VF226_21180"
     CDS             complement(5889..6806)
                     /locus_tag="VF226_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076560.1"
                     /translation="MLLYTLKRLGLALLVATFVSIVAFMLLRLSGDAAIAIAGEGARD
                     EDIELVRQTYGLDRPLIVQYADWLLTTLSGDFGQSLYFKTDVTGLIADKLPVTFYIGS
                     FSLLFALALSVPLGVLAAVYAGSWIDRAALAIAVAGQALPNFFFALVLIMLFSITLRW
                     LPVSGSDTWAHFVMPTIALGYYVTPPMMRLVRAGMIEVLSSDYIRTARAKGLSPASVI
                     FKHGLRNAVVPVVALAAVQLGYLLGGSIVIETIFALDGLGYLAYQSITHKDFPVTQAV
                     LLLLSLIYVLLTLVSDLLNAFLDPRMRVA"
     gene            complement(6977..8491)
                     /locus_tag="VF226_21185"
     CDS             complement(6977..8491)
                     /locus_tag="VF226_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076561.1"
                     /translation="MLGRSMLAAVLLASTAVMPASAAKKDNSIRFAYDQVLENIDPYF
                     NNVRLGVIMSHAVWDTLIYRNPNTNQYEPGLATSWKWIDDRTLELELRQGVKFHNGEP
                     FDADDVVYTLNFASDPANKATAQQNISWVEKAEKVDQYRVRIKTKRPFPAAIEYLAGP
                     IPMHPNEYYAKVGPKGMNEKPVGTGPYKVVEHDIGKLIRLEANKDYWEGAPKPKPTIE
                     KIEVRMIPDRQTQVAEMLSGGLDLIMNVPVEQAEQLKAAPHLAVTSGLTMRIVFILFN
                     AGENAPNKAIKDVRVRQAIAHAIDKETMAKQLVGEGARVIETICFPSQFGCTDEGAKR
                     YPYDPEKAKALLAEAGYKDGFDLELLGYRERAQTEAMVNYLRAVGIRANLKFMQYAAM
                     REQHRANKAAVTHQTWGSFSINDVSAGPSAYFKGIDDDMTKDPEVIALFEKGDTSVDP
                     EVRKEAYKRGLQLVNERAYAVPLYALTTYYVSDKDLEFKPYPDELPRFWEMKWK"
     gene            8703..8927
                     /locus_tag="VF226_21190"
     CDS             8703..8927
                     /locus_tag="VF226_21190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076562.1"
                     /translation="MTDCGTEDIAMAENGTRALKLPKSLEGHTLPQERLEVILPFVEA
                     LSETALKVGDTLPLEADPADFIRVLESEGR"
     gene            8933..>10081
                     /locus_tag="VF226_21195"
     CDS             8933..>10081
                     /locus_tag="VF226_21195"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7076563.1"
                     /translation="MDLKSLSAVELAEAIRTRKISSAEVVSSALDRLKAVNEACNAVV
                     STDAEEALAAAEKTDAAIAAGEPTGPLAGVPLAHKDMFDRVGKIPSWGARIRRDKPCN
                     EDSTLIARLKAAGSIQIAALHMTEFAFGPTGHNYVIGHARNPWDTSRIPGGSSSGSAI
                     CVATGVVPAALGSDTAGSLRIPAAACNVASMKPTWSRVSRAGAMPLSPSLDCIGPIAR
                     DVRDLALLLGLIAGHDPRDGFSSHRPVPDYLGLIDQPVDGIRIGVDERLIGETHPEVQ
                     QRFEGTLKILEKAGAKRIAVRFQDWKTLDHLGQLLQLPEVASAHGPFLRKRGADYGPQ
                     VRARMEFGHFIPGADYATALRARGTILAKVLTETFGNCDMVILPTLADP"
BASE COUNT         1730 a         3224 c         3384 g         1743 t
ORIGIN      
        1 gtaggcggag gtgatggcct tggccatcgt gatcgtgtcc ggctggatcc cgaacgtctg
       61 cgagccgaac cagttgcccg tgcgcgcgaa cccgcagatc acctcgtcgg cgatcatcag
      121 gatgtcgtag cgccgcagga cagcctggat cttctcgaag tacgtgcggg gcgggacgat
      181 cacgccgccg gcgcccatca ccggctcggc aatgaaggcg gcgatcgtct cgggatcctc
      241 gcgctggatc agctcctcca gattgctggc gaggcgggtt gcatactctt cctcgctctc
      301 gccttcctca gcataccgcc agtagtgcgg gcaatcggtg tgcagcacac gctgcatcgg
      361 caggtcgaag tcggcgtgga aaacgggcag gccggtgaga ctgcccgagg cgatggtcac
      421 gccgtggtag gctttcatac ggctgatgat cttcttcttt ttcgtcagcc cgcgcgcgtt
      481 gttgtagtac caggcgagct tgatctgcgt gtcgttcgcc tccgagcccg agctggtgaa
      541 gaagatcttg gaggctggcg cgggcaccag ctccttgagc ttctcggcca gggcgatcgc
      601 cggctcgtgg ctgcggccgc cgaacaagtg cgtgaacgac agcttgctca tctgctcgcg
      661 cgcggcttcg acgagctcct ggttgccgta gccaagaccg gtgcaccaca ggccggcaag
      721 cccttcgatg tagcgctttc cttccttgtc ccagacatag accccctcag cacgctcgag
      781 caccagaggg cctgtcgtgc gatgcagcgc aaggttcgtt gccgggtgca agatcgtctc
      841 aacgtctcgc atggcatagt tggagagggt cgtcatcgtg cctttcctgt gttggtatgg
      901 agcccgccgt cattaaccac ggcgcaagtg tctgataaag agttcgtggg cgtcctggtt
      961 cgccgggcat ctatgcaggt tttatgagca tgaccagcct ggacctttcc tggcacgctg
     1021 tccaccggat gatagacgca acccggcggc gccctgtgtt tgccgccgtc tggagcgctc
     1081 gcctggcgct gttcagcggc ggcctggcga tcgtgggcat cctccttcat cggctgctgg
     1141 gcctgccgac gccgatcttg ctcaacgtgc tgaaggtggc gttcgcgggc gccgctctgg
     1201 ccctggtgct cggcctgatg ggcgctgtgc aggtctggtt caccggcagg accggcgcag
     1261 gggccgcgac cgtcggtctg ctagccagcc tcctgctgtt cgcatggccc gcgtactatt
     1321 atcctgccat gcgggatttg ccggcgatca acgacgtcac cacggatccg gacgcaccgc
     1381 cgccgatgat ggcgctggcg aagctccgtg gtcctggcgc caatccggtg gcctaccctg
     1441 gtgagcgctt cgcagaattg caggccgccg cctatccgga cttgaagccg ttcctgatcc
     1501 cccggcctgc cgacgaggcg ttcgagatcg cagccgaggc ggtgaggcaa ttgagataca
     1561 aggtcgtttc ggaaacgcct cccggcgaga gcttcgagga gccgggctac atcgaggcgg
     1621 tggagcggac cttcgtcatg ggcttctacg acgacatagt gatccgcgtc atgggcgact
     1681 cggaggcgtc gcagatcgac atccgctcag cctctcgata cggcacgcat gatctcggac
     1741 agaacgcgag ccgaatccgc acgatcctcg ccgagcttcg gaaggtgatg gatagcaccg
     1801 taccggcaga ggcgaacccc aggaacgcgc gcagcaagca gaaggctacg cagcagaaac
     1861 ggggcggtcg aggtcgggcg ggccgtcgca attgacgagc ccgcggtcga cgaggctctc
     1921 gatgtgggcg agcacggaga gcgaggcagc cctgacgagc cggggatcaa gcccctggta
     1981 caccgcggcc acgatgcggt cgatcgtgat gtgcccttgc cggacggcat ccaggatggc
     2041 ctgctcgcgc cagcgccggt ggacgatgta ggccttcacc atccggctcg gctgctcgat
     2101 gcgcccgcca tgcccgggca gaaacaccgg ctcgctccgc tcgagcagct tttcgagcga
     2161 gcgcatgtaa tccgtcatgt tgccctctgg cggggcaacg acgctcgtgt tccagcccat
     2221 cacgtgatcg ccgcagaaga gcacgttact gctggcctcc gcgaagcaga ggtgatcggg
     2281 ggcgtgtccc ggcgtgaaca gtgcctcgag gcgccagtcc ggccccgcga ccacgtcgcc
     2341 atcccccatg acgatgtcgg gcttgaagtc gatgtcgatg aactcggcac cggacgggct
     2401 cgtcttcagg ctgcccggac cgcgcagcga ccgtccgcgg gcgcagatct tggcgccggt
     2461 cgcctcccgg atgcggtcca gcccatccgt atggtcgcga tgcgtatggg tgatgaagat
     2521 gtgcgtgatc gggcgcccgg cggcggtgcg catgatcgcc tcgaagtgcg cctcgtcctc
     2581 cggaccgggg tcgatcagcg ccagctcccg ggagccgatc agataggtgt tggtgccgtg
     2641 gaaggtgaac ggactcggat tgttggcgac gagccgcacg atgccgggcg ccagctctcg
     2701 cggcacgccg taggcgaact ccatcgtctt gttgaacggg atctcggcag tcatgcgcgc
     2761 cttgcttggc ttccgatgca gatggaaaca agccaagccc aacctccctc tccccggcgg
     2821 ggagagagtc ggggtgaggg ggttccgcca gtggcaactc ttgcaacccc tcacccggcc
     2881 cttcgggccg acctctcccc atgggagagg tgaagggggt acccgctgct gccgtttcca
     2941 cctaattcgc aagaaacgat aacacggacg gccgcacgcg aatagggggc gaggcgagac
     3001 ttcgcgggcg acggtcaggc agccctgctc gccgcattgt ccacgagatg acaggccgcg
     3061 aagccggagg cggtcgtgac tggcttcggg acgatgctcc ggcacatctc catcgcctgc
     3121 gggcagcgcg ggtggaatgg gcaacccgag ggaatgttca gcgggtcggg cgaggccgtc
     3181 ccaagcagcg cctcggggat gccgagcccg ggttccggcg tcagcaccga ggcgagcagc
     3241 gcttgtgtgt agggatggcg cgggttgcgg aacagctctg ccgtcggcgc cagctcgacg
     3301 acccggccga ggtacatgac ggccacgcgc gtcgcgagat gctcgaccac cgcgagattg
     3361 tggctgatga agacgtacgt cagccccagg ttctgcttga ggtccatcag caggttgaga
     3421 atctgcgact gcaccgagac gtcgagcgcc gacgtcggct cgtcgcagat gacgatctcg
     3481 ggttgggtca cgagggcgcg ggcgatggcg acgcgctggc gctggccgcc cgaaagctgg
     3541 ctcgggtagt tgtcggcgta gcgggaaggc aggccgacct tgtcgagcgc ggcgatcgcc
     3601 ttggcacggc gctcctcggc cgatccgatg ccctgcacct cgagcggcag cgccacgatg
     3661 gaggcgatgc gtcggcgtgg attgagggac gagtaggggt cctggaagac aggctgcatc
     3721 cgccgggcaa cctcgcggcg ggggatcgtc cgaatgtcct tcccgccgag gcgaacgctt
     3781 cctgtcgttg gcggcagcag gccgaggatc atgcgggcga gcgtgctttt cccgcagccg
     3841 gactcgccga cgatcccgag cacctcgccc ttctgcacat cgagatcgac tccggtcacc
     3901 gcattgaggg tcgcgcgccg ggcgaacatg cctcggctga cgctgaatgt cttggacagc
     3961 ccgaggacct cgatcagcgc gctcatgcga tggcctcccc gagctcggac gtggccccga
     4021 attcctcgac gcagacgtat cgatgcgtcg cggatggcga gcggattgga attgccaccg
     4081 cacacgcggc acgcgcgtag ctgcagcgcg aacggaaggc gcagccttcg atgccgccga
     4141 tcagactcgg gacgacccct gggatggtgc cgaggcgcgt gccggcctct gtgatgccgg
     4201 agcgaggcag gcacgagagc agcccccgcg tgtaggggtg gcagggcctt gcgaacagct
     4261 cctgggtcgt tgcgctttcc acgatggcgc cggcgtacat cacgtgcacg cggtcggcga
     4321 tgcgcgccac gatgccaagg tcgtgcgtga tgagcaggag cgcgatcccg agctcgcgct
     4381 gcagcccggc gagcaggcga aggatctgcg cttgaatggt gacgtcgaga gccgtggtcg
     4441 gctcgtctgc gataaggaga tccgggccgc acatcagcgc catggcgatc atcacgcgct
     4501 ggcgcaggcc gccggagagc tggtgcggat attggcctag ccgttccgcc ggcgaggcga
     4561 tgccgacctt ggcgagcaga tcgatggcgc gatcgcgtgc ctccgcaaac ccggagcgct
     4621 tatgccgccg gtaaacctcg atcagctggt cgccgatggt ccaagccggg ttcaatgccg
     4681 tcatcggctc ctggaagatc atcgccatcc gattgccgcg cagcgcattg atctcggcgc
     4741 gggaagcgcg ggtgagatcg gtgccggcga acacaaggcg gccggcgcgc cgggtcgcgt
     4801 ttgccggcag caggtccatg atggcgaggg atgtcatgga cttgccgcag ccggactcgc
     4861 ccacgagaca gacggtctcg ccgcggcgca cgtcgaagct cacgccccgc acggcatgga
     4921 gcggccccgc aggcgtgcgg atcgtcacgg aaagatcctg gacgtcgagc acgggttcgg
     4981 gtgacacgcc tgtcatcatg ccctttcatc cggcgcaaac acgtctcgga ggccgtcgcc
     5041 cagcaggttg atgccgagga cgagcaacgc gagggccgtg ccagggatgg caatgagcca
     5101 gaacgagaag aacatgaagt tcttcgcttc tgcgatcatc agcccccagg acggaagtgg
     5161 cggctgcact ccgaggccca ggaacgagag cgccgcctcg agcaggatcg cgctgcccgc
     5221 ttcgacggtc gctacgacga tcaggtgcgg aaaaacattg ggcaggacct cgccgagcag
     5281 gattctgagc cgcgaagccc ctgccgcttc ggcagccgtg acatagtcga gggagcggac
     5341 ctgctgggtc gcgctgcgca tgacgacggc gaagcggtcc catttgagca ggccgagtgt
     5401 caggatcacc acgggaagcg agccgccgat catggcgacg acggcgagcg ccaccagcac
     5461 caccggcatg gcgagccgcg cggtgatgaa gaagctgatg acgaggtccg tcctgccgcc
     5521 gaaatagccc gccgcaagcc cgagcgccgt tccgatcagc cctgagatga ccatcaccga
     5581 caggccgatc agcagcgaga tgcgggcgcc gtacaggatg cgcgaaaggt agtcacgtcc
     5641 cagattgtcg gtacccagcg ggttcgacca gatgcctttg tcgtgccaga tcggcggaac
     5701 gagcctgcgc atcagatcct gtgcataggg atcgtgcggc gcgagcagcg gtgccagcag
     5761 ggcgatcgcc acgacagcgc caacgaggat cagcccagtc acgaccgaag ggttgccggc
     5821 catgcgggcc gccgccgagc cggccttcgg cacgacggtc tcgacaggga ggatgactgt
     5881 gtcgctcatc aggccaccct catcctggga tcgaggaatg cgttgagcag gtcggaaacg
     5941 agtgtcagca gcacatagat cagcgacagc agcaggagga ccgcctgcgt caccggaaag
     6001 tccttgtgcg tgatcgactg gtaagcgagg tagcccaacc cgtcgagcgc aaagatcgtt
     6061 tcgatgacga tggagccgcc gagcaggtag ccgagctgga cagcagcaag cgccaccacc
     6121 gggacgaccg cgttgcgcag gccatgcttg aagatgacgc ttgccggcga caggcccttg
     6181 gcgcgcgcgg tgcggatgta gtccgagctc agcacctcga tcatgccggc gcgcacgagg
     6241 cgcatcatcg gtggcgtcac gtagtagccg agcgcgattg tgggcatcac gaaatgggcc
     6301 cacgtatcgc tccccgagac cggcagccat ctgagcgtga tcgagaacag catgatcagc
     6361 acgagggcaa agaagaagtt cggcaacgcc tgtccggcca ccgcgatggc gagtgcagcg
     6421 cggtcgatcc agcttccggc atagaccgcc gccaggactc cgagcggcac ggagagtgcc
     6481 agcgcgaaga ggagggagaa gctgccgatg tagaaggtga ctgggagctt atccgcaatc
     6541 agccctgtga cgtccgtctt gaagtagagc gactgtccga aatcgccgga aagggtcgtc
     6601 agcaaccagt ccgcgtattg cacgatgagc ggccggtcga ggccgtaggt ctggcgaacg
     6661 agctcgatgt cttcatcccg tgcgccttcg ccggcaatgg cgatcgccgc gtcgcccgac
     6721 aagcgcagca gcatgaatgc cacgatcgag acgaaggtgg caacgagtag ggcgaggccg
     6781 agccgcttga gcgtgtagag cagcatcaaa ccctcccgtg cagcgcttca gcctgcactt
     6841 tggcaaccca ctgtgaaagc cgtccgcgcg actctcaccg caaatcccgg atctgcccgc
     6901 cttctcgact tttcgtttcc gggatgacgg aaaatgcttc gcggtggccc gccctggagg
     6961 ccaaccgcga tcgacctcac ttccacttca tctcccagaa ccgggggagc tcgtcggggt
     7021 atggcttgaa ctccaaatcc ttgtcggaaa cgtagtaggt ggtgagcgca tagagcggca
     7081 cggcgtaagc ccgctcgttc acgagctgca ggccgcgctt gtaggcctcc ttgcgcacct
     7141 ccggatctac gctggtgtcg cccttctcga agagcgcgat cacctccggg tccttggtca
     7201 tgtcatcgtc gatgcccttg aagtaggcgg acgggccggc ggagacgtcg ttgatgctga
     7261 acgagcccca ggtctggtgc gtgactgcgg ccttgttggc gcgatgctgc tcgcgcatgg
     7321 cggcgtactg catgaacttg aggttcgccc gaatgccgac ggcgcgcaga tagttcacca
     7381 tcgcctcggt ctgtgcccgc tcacggtagc cgagcagctc caggtcgaaa ccatccttgt
     7441 agccggcctc cgccaggaga gccttggctt tctccggatc ataaggatag cgcttcgcgc
     7501 cctcgtccgt gcagccgaac tggctcggga agcagatcgt ttcgatcacc cgcgcgcctt
     7561 cgccgacgag ctgcttggcc atggtctcct tgtcgatggc atgcgcaatc gcctggcgca
     7621 cacgcacgtc cttgatcgcc ttgttgggcg cattctcgcc ggcattgaac aggatgaaga
     7681 cgatgcgcat cgtcaggcct gacgtgaccg ccagatgggg cgccgccttg agctgctcgg
     7741 cctgctcgac gggcacgttc atgatcaggt caagcccgcc ggaaagcatt tcggcgacct
     7801 gggtctgccg gtccgggatc atgcgcacct cgatcttctc gatcgtcggc ttcggcttcg
     7861 gcgcgccttc ccagtaatcc ttgttcgctt cgagccggat caacttgccg atgtcgtgct
     7921 ccacgacctt gtacgggccc gtgccgaccg gcttctcgtt catgccctta gggccgacct
     7981 tggcgtagta ctcgttcgga tgcatcggga tgggcccggc gaggtactcg atggccgccg
     8041 ggaatggccg cttggtcttg atccgcactc tgtactggtc gaccttctcc gccttctcca
     8101 cccagctgat gttctgctgc gctgtcgctt tgttggcggg atcggaggcg aagttcagcg
     8161 tgtagacgac gtcgtcggcg tcgaacggct cgccgttatg gaacttgacg ccctgcctca
     8221 gctcgagctc gagggtgcgg tcatcgatcc atttccagga tgtggccaat cccggctcgt
     8281 actggttggt attgggattg cggtagatga gcgtgtccca gaccgcatgg ctcatgatca
     8341 cgccgagccg gacgttgttg aagtagggat cgatgttctc gagcacctgg tcgtaggcga
     8401 agcggatcga gttgtccttc ttcgcggcgc tcgcgggcat caccgccgta gaggcgagca
     8461 ggaccgccgc gagcattgag cgtccgagca tttccgattt cctcccgatc tcaattgcct
     8521 tctttggttg gccgatttga gcacgctcga atgggcgggc gtcaactgct ttttccgggc
     8581 gcggcaacac catgcgcgca gcgcccccgg ccagacccta gccttcccaa gttaaccggt
     8641 cgttctgacc caggccagtt gcacgttatc ggccttctcg ggcatgctgg ctccgaacgg
     8701 gaatgactga ttgcgggacg gaggacatcg ccatggccga gaacgggacc cgggcgctca
     8761 agctgccaaa gagcctggag ggtcacacgc tgccgcagga gcggctggag gtcatcctgc
     8821 cgttcgtcga ggcgctgtcc gaaacggcgc tgaaggtggg cgatacgctg ccgctcgagg
     8881 ccgatcccgc cgatttcatc cgcgtgctcg aaagcgaggg gaggtaggca ccatggatct
     8941 caagtctctc tccgccgtcg agctcgccga agccatccgc accaggaaaa tctcgagcgc
     9001 cgaggtcgtc agcagcgcgc tggaccggct gaaagccgtc aatgaagcct gcaatgccgt
     9061 cgtctccacc gatgccgagg aggcgctcgc cgccgccgag aagaccgatg ccgccatcgc
     9121 tgccggcgag ccgacggggc cgctggcggg cgtgccgctc gcgcacaagg atatgttcga
     9181 tcgcgtcggc aagatcccga gctggggggc ccgcatccgc cgcgataagc cgtgcaacga
     9241 ggacagcacg ctgattgccc ggctcaaggc cgcgggcagc atccagatcg cggcgctgca
     9301 catgaccgag ttcgctttcg ggccgacggg gcacaactac gtcatcggcc acgcgcgcaa
     9361 tccgtgggac acctcccgca ttccgggcgg ctcgtccagc ggctcagcga tctgtgtggc
     9421 gacgggcgtc gtgccggccg cgctcggctc cgacacggcg ggctcgctgc gcatcccggc
     9481 ggccgcctgc aacgtcgctt ccatgaagcc gacctggagc cgcgtgtcgc gcgccggcgc
     9541 catgccgctg tcgccctcgc tcgactgcat cggcccgatt gcgcgcgatg tccgcgatct
     9601 cgcgctgctg ctggggctga tcgcaggaca tgatccgcgg gatggcttca gctcgcaccg
     9661 tccggtgccg gactatctcg gcctcatcga ccagccggtg gacggcatcc gcatcggcgt
     9721 cgatgagcgg ctgatcggcg agacgcatcc cgaggtgcag cagcggttcg aaggaaccct
     9781 caagatcctg gagaaggctg gtgcgaagcg cattgccgtg cgcttccagg actggaagac
     9841 cctcgaccac ctgggccagc tcctgcagct acccgaggtc gcctcggcgc acggaccatt
     9901 tctgcgcaag cgcggcgccg attacggccc gcaggtgcgt gcgcgcatgg agttcggcca
     9961 cttcatccct ggcgcggatt acgcgacggc gctgcgcgcc cgcggcacca ttctcgccaa
    10021 ggtgctgacg gagaccttcg gcaattgcga catggtcatt ctgccgaccc tcgccgatcc
    10081 g
//
LOCUS       DASVDU010000318         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_62671, whole genome shotgun sequence.
ACCESSION   DASVDU010000318 DASVDU010000000
VERSION     DASVDU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62671"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..379
                     /locus_tag="VF226_21200"
     CDS             134..379
                     /locus_tag="VF226_21200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076564.1"
                     /translation="MISAAQLRAARALLGIDQRTLAEMAGLSLPTIQRMEASEGVIRG
                     NVDSLTKLVSALNAAGIELIGENAPSVGKGRGVRLME"
     gene            457..2463
                     /gene="hyfB"
                     /locus_tag="VF226_21205"
     CDS             457..2463
                     /gene="hyfB"
                     /locus_tag="VF226_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX7076565.1"
                     /translation="MAIGAVLTCMAALLALAAVGVVTHNRPIATRVIYSASLALSSVI
                     LIVAIVRLLTWSGPDPAITLPIGLPWIGARFRIDALSAFFLIVVNLGAAAASLYALGY
                     GEHEKQPGRVLPFYPAFLAGMNIVVLADDAFSFLVAWEFMSVASWALVVAHHQEAENT
                     RAGYIYLLMASFGTLTLLLTFGLLAGPAGGYGFDDIRAAQHASWLPGFVLALALIGAG
                     SKAGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRIVFDLLGGPGWWSGMIV
                     LALGGITAVMGVLYALMQHDLKRLLAYHTVENIGIIFIGLGLALAFGANGMQGAAALA
                     LTAALFHVLNHSLFKSLLFFGAGAVLTATGERDMEHLGGLIHRMPLTSFAFLAGCVAI
                     SALPPLNGFVSEWLTFQAILQSPDLPQWGLKILVPAVGGLLALSAALAAACFVKAFGV
                     TFLGRPRSEAAVKAAEVDQFSLSTMLGLAVLCLVLGILPGLVIDALAPVTLSLTGGRM
                     PAQLSVAWLSIVPIAESRSSYNGLLVFLFIAFSAWAAVLVIHRYASHALRRGPAWDCG
                     FPDFRPATQYTASSFAQPIRRVFGTLVFRAREQVEMPPPGSPEPARLTVKLRDLVWDG
                     TYVPIADAVSLAADRLNVLQFLTIRRYLSLVFLALVALLLVLAL"
     gene            2460..3413
                     /locus_tag="VF226_21210"
     CDS             2460..3413
                     /locus_tag="VF226_21210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX7076566.1"
                     /translation="MILLELFIQGVQMALVLALAPLLTGFVRKVKARLLRRRGPPLLQ
                     PYRDLLRLLRKEVVLAENASWLFRVAPYMIFAATWVAAALVPTFATGLVFNWTADLIA
                     IIALLASARFFLALAGMDVGTSFGGIGSSREVMIASLAEPAMLLIVFTLALVAGSTQL
                     STVAAYMTLPEVGLRVSLALALFGLIMVAIAENARIPVDNPATHLELTMVHEAMVLEY
                     SGRHLAMIEFAAFLKLLLYVSLIACLFVPWGMASPGAGPTAYAVGVVTYVAKLAAGGF
                     LLAVFETAIAKMRVFRVPEFLGAALMLGLLGTLLLFVSRSL"
     gene            3413..4075
                     /locus_tag="VF226_21215"
     CDS             3413..4075
                     /locus_tag="VF226_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component E"
                     /protein_id="HEX7076567.1"
                     /translation="MDRLAFDIAHLLAGGLVLVSLTMLYQDRLNALLNVYALHALVLA
                     LSVAWQAFIQDAPHLYVTAAIALLFKAIVIPVALHRIIQRLGIHREIEPVIGIGLTML
                     VGIGLVALSIVVMLRVTENAAPLARQDLAFALSVVLLGLLVMVTRRNAVGQVVGFMSL
                     ENGLVLAATGAKGMPLVVEISVAFSILIAFIVIGIFLFRIRERFDTVDIRALDQFRGE
                     RR"
     gene            4072..>4936
                     /locus_tag="VF226_21220"
     CDS             4072..>4936
                     /locus_tag="VF226_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017992149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7076568.1"
                     /translation="MSEFLMPDLAFEPVTALLLIPGISAIILALLPGYKLTARLNVLA
                     AGLTFLASLWLLVVRPDTGDYLLVDDLNVVFVVLGTFVGFTTSVFSASYIGHELNIGR
                     LTPANLRFYHAMYQALMFAMNLALLANNIGLMWVAVELATLTTVLMVGIYRTHEALEA
                     AWKYFILGSVGIALALFGTILVYMAAQPVMGAGQDAMVWTSLITKAAEFSPDLLNLAF
                     IFLMLGYGTKVGLAPLHAWLPDAHAEGPTPISAVLSGLLLNVALYAVLRFKMLLAANP
                     EAISPGPLMVVL"
BASE COUNT          686 a         1653 c         1540 g         1057 t
ORIGIN      
        1 tcatcgagca ttgtcagacg gcaacgcgtc cgcggtaaac cggcacgcct gcgcacgagg
       61 ggcttgacgt ttcagtagtg aaggctgata accctgaact cacgttccga gggcaatttc
      121 tgatgtctca aacatgattt ctgccgcgca gcttcgtgcg gcgcgggctc tgctcgggat
      181 cgaccagcgc acgctggccg agatggcggg tctgtcactg ccgacaattc agcgcatgga
      241 agcgagcgaa ggcgtgatcc gcggcaacgt ggattccctc accaagctcg tctctgcact
      301 gaatgccgcg ggcatcgagc tgatcggtga gaacgctccg agcgtgggca agggtcgagg
      361 cgttcgcctc atggaataag tgtcatacgg gggacgatgg ccaggcgtaa cgccctcctg
      421 ccggccacag cctgaaagat gcggagataa gtgcaaatgg cgatcggtgc ggtgctcacg
      481 tgcatggcgg cactgcttgc gctcgcggcg gtcggtgtgg tcacacacaa caggcccatc
      541 gccactcgcg tgatttactc cgcttctctc gctctctcct cggtcattct gatcgttgcg
      601 atcgtccggc ttttgacgtg gtccggcccg gatcccgcga tcactctgcc cattggcctg
      661 ccctggatcg gtgcgcgttt ccgcatcgat gcgctgtcag ctttctttct catcgtcgtt
      721 aatctcggcg ccgcagccgc gagcctttat gcgctgggat acggtgagca tgagaagcaa
      781 cccggacggg tgctgccgtt ctatccggca ttcctcgccg gcatgaacat cgtcgtcctc
      841 gcggacgatg ccttcagctt cctcgtcgca tgggagttca tgtccgtcgc gtcctgggcg
      901 ctcgtcgtgg cacaccatca ggaggcggag aacacgcggg ctggttacat ctatctcttg
      961 atggcgagct tcggcacgct gacgctgctc ctcaccttcg gcctgctcgc cggtcctgcg
     1021 ggcggctacg ggtttgacga catccgcgcg gctcagcacg cctcctggtt gccggggttt
     1081 gtcctcgcgc tggcgctgat cggcgctgga tcgaaggcgg gtctcgtgcc gctgcacgtc
     1141 tggctgcccc tcgcccaccc cgccgctcca agccacgtct cggcattgat gagcggcgtt
     1201 atgacgaagg tggcagtcta cggcttcgtg cgcatcgtct tcgacctgct cgggggcccg
     1261 ggctggtggt cgggcatgat cgtgcttgct ctcggaggca tcaccgccgt gatgggtgtg
     1321 ctctacgccc tcatgcagca cgacctcaag cgcctgctcg cctatcacac cgtcgagaat
     1381 atcgggatca tcttcatcgg gctggggctt gcgcttgcct tcggcgccaa cggcatgcaa
     1441 ggggcggcgg cattggcgct caccgcggcg ctcttccacg tcctcaatca ctcgctgttc
     1501 aagagcctgc tgttcttcgg cgcaggcgcc gtgctgacgg ccacgggcga gcgcgacatg
     1561 gagcaccttg ggggccttat tcaccgcatg ccgctgacga gcttcgcttt cctggcgggc
     1621 tgcgtcgcga tctcggcgct gccgccgctc aacggcttcg tctcggagtg gctgacattc
     1681 caggctattc tgcagagccc cgacttgccg cagtggggcc tcaagatact ggtgcccgcg
     1741 gtgggcgggc tgctcgcgct ctccgcagct ctggccgccg cctgcttcgt caaggctttc
     1801 ggcgtgacct ttctcggccg gccacgctca gaggcggcgg tgaaggcggc ggaggtcgac
     1861 cagttctcgc tcagcaccat gctcggcctc gcggtgctct gcctcgtcct cggcattctg
     1921 cccggccttg tcatcgacgc gctggcgccg gttacgctgt ccctgaccgg cgggcggatg
     1981 cccgcgcagt tgagcgtggc atggctctca atcgtaccta ttgccgaaag ccggagctcg
     2041 tataacggac tactggtgtt cctcttcatc gccttttcag cctgggcggc agtgctggtg
     2101 atccaccgct acgcctccca cgcgctgcgg cgagggcccg cctgggactg cggctttccc
     2161 gacttccgtc cggcgacgca gtacacggcc agcagcttcg cccaacccat ccgccgcgtc
     2221 ttcggcacgc tcgtcttccg cgcccgcgag caggtcgaga tgccgccgcc gggcagtcct
     2281 gagccggcgc gcctcaccgt caaattgcgc gatctcgttt gggacggcac ctacgtgccg
     2341 atcgccgacg ccgtcagctt ggctgcggac cgcctcaacg tcctgcagtt cctcaccatc
     2401 cggcgctatc tcagcctcgt cttcctcgcg ctcgtcgcgc tgctcctggt gctggcgcta
     2461 tgatcctcct cgagcttttc atccaaggcg tgcagatggc gctggtgctc gcgctggcgc
     2521 cgctgctcac cggcttcgtg cgcaaggtga aggcacgttt gctgcggcgc cggggcccgc
     2581 cgctgctaca gccctaccgc gatctcctgc gtctcctgcg caaggaggtg gtgctggcgg
     2641 agaatgcctc ctggctcttc cgcgtcgcgc cgtacatgat cttcgcggca acctgggtcg
     2701 cggctgcgct ggtgccgacc ttcgcgacgg gcctcgtatt caactggaca gcggacctga
     2761 tcgcgatcat cgcactcctc gccagcgcgc gattcttcct ggcgctggcc ggcatggacg
     2821 tcggcaccag cttcggcggc atcggttcga gccgcgaggt catgatcgca tcgctcgccg
     2881 agcccgcgat gctgctcatc gtctttaccc tggcgctggt tgccggctcg acccagctgt
     2941 cgacggtggc cgcctacatg accttgcccg aggtcggcct gcgcgtctcg ctggcgctgg
     3001 cactgttcgg tctcatcatg gtggcgatcg ccgagaatgc ccggatcccg gtcgacaatc
     3061 cggcgaccca tctcgaactg accatggtgc acgaggcgat ggtgctggaa tactccggcc
     3121 gccacctggc gatgatcgag ttcgcggcct tcctcaagct cctgctctac gtctcgctga
     3181 ttgcctgcct gttcgtgcct tggggaatgg cgtcaccggg cgcgggtccc accgcctacg
     3241 ctgtgggcgt ggtcacctat gtggcgaagc tagcggcggg cggattcctg ctcgctgtgt
     3301 tcgagacagc catcgccaag atgcgggtgt ttcgcgtgcc cgaattcctc ggtgctgcgc
     3361 tgatgctggg cctccttggc acgcttctgc tgttcgtctc gcggagtctc tgatggaccg
     3421 tctcgccttc gacatcgcgc atctcctcgc cggcggactc gtgctcgtca gcctgacgat
     3481 gctctaccag gaccgtctca acgcgctcct caacgtctac gcgctacacg cactggtgct
     3541 ggcgctctcg gtcgcctggc aagccttcat ccaagatgcg ccccaccttt atgtcacggc
     3601 ggcgatcgcc ctcttgttca aggcgatcgt catccccgtg gccctgcatc gcatcatcca
     3661 gcgcctcggc atccaccgcg aaatcgagcc ggtgatcggc attgggctga cgatgctcgt
     3721 cgggatcggg ctcgtcgcgc tttcgatcgt cgtgatgctg cgggtgacgg agaatgccgc
     3781 ccccctggca cgccaggacc tcgccttcgc cctgtccgtg gtgctcctcg ggctcctcgt
     3841 catggtgaca cggcgcaatg cggtaggcca ggtggtgggg ttcatgtcgc tcgagaatgg
     3901 cctcgtgctg gccgccacgg gtgcaaaggg catgccgctg gtcgtcgaga tcagcgtcgc
     3961 cttctcgatc ctgatcgcct tcatcgtgat cggcatcttc ctattccgta ttcgcgagcg
     4021 cttcgacacg gtcgatatcc gggcgctcga tcagttccgg ggtgagcgca gatgagcgag
     4081 tttctcatgc ccgatctcgc gttcgaaccg gtgacggcgc tgctgttgat tcccggtatt
     4141 tccgcgatca tcctggcgct gctgccggga tataagttga cggcacgcct caacgtgctc
     4201 gcggccgggc tgacattcct cgcctcgctg tggttgctgg tggtgcgccc agacaccggc
     4261 gattatctgc tggttgacga tctcaacgtt gttttcgtcg tgctcggaac attcgtcggc
     4321 ttcacgacga gcgtgttcag cgccagctac atcggccacg agctgaacat cggacgactg
     4381 actccggcga acttgcgctt ctaccacgcg atgtatcagg cgctgatgtt cgccatgaac
     4441 ctcgcgctgc tggccaacaa catcggcctc atgtgggtgg ccgtggagct ggcgacactc
     4501 acgacggtgc tgatggtcgg catctatcgc acgcacgagg cgctcgaggc ggcctggaag
     4561 tatttcatcc tcggcagcgt cggcattgcg ctcgcgctct ttggcaccat cctcgtctac
     4621 atggcggcgc agcccgtcat gggcgcaggt caggacgcga tggtttggac atcgctgatc
     4681 acgaaggcgg ccgagttcag cccggacctg ctgaacctcg cattcatctt cctgatgctc
     4741 ggctacggca ccaaggttgg cctcgcgcca ctgcacgcgt ggctgccaga cgcgcatgcg
     4801 gaaggtccga cgccgatctc ggcagtcttg tcaggcctgc tgctgaacgt ggcgctctac
     4861 gccgtgttgc gcttcaagat gctgctggcc gccaaccccg aggcgatctc tcccgggccg
     4921 ctgatggtgg tgctcg
//
LOCUS       DASVDU010000319        26231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1255640, whole genome shotgun sequence.
ACCESSION   DASVDU010000319 DASVDU010000000
VERSION     DASVDU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 26231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26231
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1255640"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VF226_21225"
     CDS             <1..799
                     /locus_tag="VF226_21225"
                     /inference="COORDINATES: protein motif:HMM:NF013548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7076569.1"
                     /translation="LADGMVVRAPAPPPPPPVVDRCAPGPWTGFYVGGNLGWAHLDGE
                     FNDHDHFFSDRRWFDDDEDAFTAGVQTGYNLQCGNVVFGVEGDINWAGFDDDNDHFGH
                     FRDGKLKRERFKRDRSMDWFGTIRGRLGWASDRIMVYATAGAAYTELDRGHRGPGRDW
                     NSDLSIWGWNDHHDDSDDVNWGWTAGGGVEWLWSPNLSFKAEALWIDFEDGDNDRDIK
                     FHANHKGTLVTWHDRKRFDHDDDMVVVRVGLNYKFGYRAPPPYEPLK"
     gene            complement(874..2004)
                     /locus_tag="VF226_21230"
     CDS             complement(874..2004)
                     /locus_tag="VF226_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX7076570.1"
                     /translation="MVLRHRARPGFDLDCIIVGDCLEELSKLPTASVDLVFADPPYNL
                     QLDGDLLRPNNTRVDGVDDDWDKFSDFAEYDRFSRAWLAECRRVLKRDGAIWVIGSYH
                     NIFRLGAALQDLGFWIQNDIIWRKTNPMPNFRGKRFTNAHETLIWAGRDQKSRVTFNY
                     EAMKAGNDDLQMRSDWLFPICAGPERLKDASGRKAHPTQKPEALLHRILLATTNPGDV
                     VLDPFFGTGTTGAVAKRLGRRWIGIERDPEYARAASERIERVRPVSPTALETARSKRT
                     EPRVPFGTIVELGILEPGSLLFDERRRVRAEVRADGTLAWQGEQGSIHRLGAAVQGKS
                     ACNGWTFWHFEAEGQLKPIDALREMAKRQLGLSGRPVVIAAE"
     gene            complement(2105..2800)
                     /locus_tag="VF226_21235"
     CDS             complement(2105..2800)
                     /locus_tag="VF226_21235"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7076571.1"
                     /translation="MAGDDSKPRATFEVEAVSIRLTGGPVAGIDEAGRGPLAGPVVAA
                     AVILDPERIPSGIADSKTLEPEVREELFAQIMATSRVGIGVASVARIDRYNILNATLW
                     AMRQAFRQLDPKPRVALVDGNRPPKLSCDTQCIVEGDAKCLSIAAASIIAKVTRDRMM
                     VELGRAFPGYGFERHKGYSTPEHFAAINRLGVTKHHRRSFRPVQVALGLVPPDELEVA
                     QLLPGFDPISDQP"
     gene            complement(2833..3948)
                     /locus_tag="VF226_21240"
     CDS             complement(2833..3948)
                     /locus_tag="VF226_21240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX7076572.1"
                     /translation="MSAAPNNPAHPAAAQLVDADRRRGRGARSNRVGRYETELREEFD
                     DGWESLAELEPFKTQVRIEVARSIIASNDSPDIGFDRSINPYRGCEHGCIYCYARPTH
                     CYLGHSAGLDFETKLYAKTNAAELLEKELSRPGYRPSTIALGAVTDPYQPIEREYCIT
                     RSILEVLDRTNHPVGIVTKSALVTRDIDILAPMAERGLVKVALSVTTLDRYVARKMEP
                     RASTPARRLEAIRQLSSAGIPTQVMVAPIVPALNDSEIERILQAAHEAGARDAGYVLL
                     RLPLELKELFREWLATEVPDRAKRVIGVLQSMHGGRDYTSQFGIRQRGSGPYADQIAT
                     RFRLALRRLGMNERGLKLRTDLFRKPTGSYKGQLQLL"
     gene            4067..4756
                     /locus_tag="VF226_21245"
     CDS             4067..4756
                     /locus_tag="VF226_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX7076573.1"
                     /translation="MISVIIPTLNAEATLASTLASLVPAAVDGVVRQVIVADGGSTDR
                     TLEIADAAGADIVQGEAGRGRQLARGAKAARSAWLLFLHADTVLEPGWHTEAAAFMDG
                     IETDHRPVSAAAFRFTLDDAGMAPRLIEAAVWLRCALARLPYGDQGLLLPARLYREIG
                     GFRPMPIMEDVDIVRRLGWRRLTMLSSRAVTSASRYRRDGYGRRTARNLSCLALYALR
                     VPPDTIARLYR"
     gene            4878..5846
                     /locus_tag="VF226_21250"
     CDS             4878..5846
                     /locus_tag="VF226_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7076574.1"
                     /translation="MTLLSRRSFVVSAATASAAFGLKGRLAFINPVQAQSTDLPRFAR
                     FAIGDIEVTQLYDGVWHKAHDPNFIAGVSVEETKKALRDANLTDAHVPITFTITVLRM
                     GDKLVMFDSGTGGQMAPTAGLLMKESMQAAGIDPKSISTIIMTHFHPDHIFGLMEKGT
                     NAQVFPDAELVVPASEYKYWTDEGVIATLPEARQGLAQRIQATFPKWKNVRQVENDAE
                     VVPGVRSVASNGHTPGHTSYLVSSGSDQLMVLGDVTNLPALFVRNPGWHASFDQDPEM
                     AESTRRKMFDRVIADKITITGYHYGMPGAGTIEKDGSGYAYKPISA"
     gene            5944..6564
                     /locus_tag="VF226_21255"
     CDS             5944..6564
                     /locus_tag="VF226_21255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX7076575.1"
                     /translation="MAKLPVAGSVKTRLAREIGTVRATWFYRHAAAAVIARMARSRIW
                     ETVLAITPDSSVAHPTWPRGLTRMGQGRGDLGRRMQRAIRLQPPGPVVIIGTDVPGIR
                     PSDVAAAFKSLGSHDAVFGPASDGGYWLVGLRRRPREIAMFKDVRWSSPHALQDTLRN
                     LRCYRIGFVAEKSDVDDAADLRRVTTCFGRRVLPALADELTSPKNL"
     gene            6628..7287
                     /locus_tag="VF226_21260"
     CDS             6628..7287
                     /locus_tag="VF226_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076576.1"
                     /translation="MVQASPQVTAAQVLDAGRRAEADGRVEYAVQFYRHLTDHHSGAP
                     EAAAAREALSRLKRQGNLSDQVSRMRPANPPPLRSDHRGADEMKRVGPKVGRGPIRIA
                     PVGAGQAPVRMELPVPPADYFTGRLIAYAFAGVGGLLFLAGLLLAIAVALMPAEVAAR
                     IPAWMPVFHPLTGTGAAMLGLVLVFSGQVGRALFDLAIASRDIAAIERAKLEHAIAEA
                     R"
     gene            complement(7290..7838)
                     /gene="moaB"
                     /locus_tag="VF226_21265"
     CDS             complement(7290..7838)
                     /gene="moaB"
                     /locus_tag="VF226_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX7076577.1"
                     /translation="MARIDETRPFIPVSIAVLTMSDTRALEDDKSGATLAELIEKAGH
                     KLAARALVRDDIPQIRAQVLRWVADPDIDVVITTGGTGFTGRDVTPDALKPLFEKEID
                     GFSTVFHLISYEKVGTSTIQSRACAGLIGETYVFCLPGSPGACRDAWNGILQYQLDNR
                     HRPCNLVEIMPRLGEHRKKAGA"
     gene            complement(7868..8779)
                     /locus_tag="VF226_21270"
     CDS             complement(7868..8779)
                     /locus_tag="VF226_21270"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7076578.1"
                     /translation="MEHSPDPRLIALLAWYREAGVTDALSDEPLNWFARGDAAVLTPE
                     PAEPAAARSVSQLPAPETTFSESFQIPSRGPSRGPTAVPARSFATVPATEAEDSAKAA
                     AGSARSLDELRAALETFEGCALRQTAKNLCFYRGAPQARVMIIGEAPGREEDLEGKPF
                     VGRAGQLLDRMLNAIGLSEADTHITNIVYWRPPGNRTPTPQEAIVCRPFLVRQIELVR
                     PDFLLLLGAAATRHILGGTEGILRIRGKWREVEIGSRRIPVMATLHPAYLLRTPASKR
                     LAWRDLLALRARLGEGNSPDAADEGSA"
     gene            8921..10603
                     /locus_tag="VF226_21275"
     CDS             8921..10603
                     /locus_tag="VF226_21275"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX7076579.1"
                     /translation="MAREPEQLPPREAMDFDVVIVGAGPAGLAAAIRLKQLALADGNE
                     ISVVVLEKGSEVGAHILSGAVIDPVGLDALIPDWKERGAPVDTPVTEDRFLYLGPAGE
                     LKLPNFLMPPLMNNHGNYIVSLGNLVRWLGEQAVELGVEVYPGFAATEVLRDESGAVI
                     GVATGDMGVGRDGKPKDNFTRGMELRGKYTLIAEGAHGSLAKQLIKDFNLREGREPQK
                     YGIGIKELWEVSPERHRPGLVQHTFGWPLDNRTGGGSFLYHYGENLVAVGFVVHLNYE
                     NPYLSPYHEFQRFKTHPVIRPHLEGGKRIGYGARAITEGGWQSLPKLVFPGGALLGCS
                     AGMVNVPRIKGSHNATLSGMHAADAVSDALKHGRSHDTLDAYERLVRTGPIARDLRPV
                     RNVKPMWSRFGSFAGIALGGIDMWLNTILPGVGLGYTLRHGKPDYATLNKAAKAKPID
                     YPKPDGVLTFDRLTNVAFSGTNHEEDQPVHLVLQDPSIPISVNLPEYDEPAQRYCPAA
                     VYEVVMDEDGRPRFQINAQNCVHCKTCDIKDPSQNINWVPPEGGGGPNYQGM"
     gene            complement(10644..12560)
                     /locus_tag="VF226_21280"
     CDS             complement(10644..12560)
                     /locus_tag="VF226_21280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7076580.1"
                     /translation="MFGKPGRSEAEPMCTCDPDMRHTGDRTLELERAVHVLRQQLAKA
                     ESALASYKKGTGGQAPWPTDALPADHQSSLEAFRLLVEHMSDGVLMLTADGAIRYCNE
                     AVARLLGLPKNRILGRSFGEFVLGEPSHFLQRLVLSDSDSMQTSLRTLSGTSVPVQIS
                     RTRSLTIDGQTLYGLVVTDLSRHVLRLRHEAVVQASKDAIYVLSPSLQIETWNRGAET
                     IFGYSPSEIIGKSERDLCPRSELPALDDLVRRVQESGAAVTVDATRRRKDGSKIRVIL
                     SLTPIRELVGRTIGYAVVAHDVTERDRAARQLREAMARTSLALAAGRMGTYELDFATN
                     RCSWSDHVYAIYGLDKATFEPTIENTSALIHPDDRELIWQNLEAAKLSGSLELEARVR
                     HQDGRYIWIHSRGLVRYDAQGNPQAIFGVIADITERKEAERRQQLLVGELNHRVRNTL
                     ATVQAIASQTLRRAKSPDDFVPSFSERIQALSRAHTLLTRTAWQGTELDALIREQLML
                     GSADRISCEGPQVALDPQTALQLALVLHELGTNARKYGSLSVPQGTVDVHWTLEQAGG
                     SSVLHLTWRERGGPPVTPPASTGFGTLLIERSLQQAQGGSSRISYDPEGVTCAIRLPL
                     SPSDAAASAHHPSA"
     gene            complement(12915..13553)
                     /locus_tag="VF226_21285"
     CDS             complement(12915..13553)
                     /locus_tag="VF226_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX7076581.1"
                     /translation="MSQFPEHLADRFRRFKFRHFAPNFDRYEELAQYGQHPDVMVVSC
                     CDSRVDPETLFSAMPGELFVVRNVANLVPPFETSGTYHGVSAALEFAALNLRVKHIVV
                     LGHSGCGGISACLEESAARQSEAQFISNWMAILEDARRKVLAARGDQSIPELRSRLER
                     LGIKTSLQNLRSFPCIKSLESKGRLHLHGAHFEIATGQLAVLNQGTGEFVSI"
     gene            complement(13641..14471)
                     /gene="pdxY"
                     /locus_tag="VF226_21290"
     CDS             complement(13641..14471)
                     /gene="pdxY"
                     /locus_tag="VF226_21290"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HEX7076582.1"
                     /translation="MARVLAFSSQVVRGHVGLSAIVPALQRLGHEVWPLPSIILSNHP
                     GHPHAAGMRVETAVLEEVVDALDRNGWLGEIDAAITGYLPSAEHACLAASVIRRLRAN
                     GKLTYLCDPVLGDEPKGLYIEAAAAAAIRDELVPLADIITPNRFELSWLSDIPIKTDD
                     VLPNAARGLAPATVLVTSARRRGPMLENLLIDEDGVKACEVPYRESVPHGTGDLFSAL
                     VLGHHLNGATETSAMAFAAAGVEQAILASAGRDELALVASQEAWVAAKPLEVSQWHAG
                     "
     gene            complement(14483..15670)
                     /locus_tag="VF226_21295"
     CDS             complement(14483..15670)
                     /locus_tag="VF226_21295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7076583.1"
                     /translation="MADLSTDIVIVGGSVIGCAAAYYLAQTGVLDERRVVIVERDPSF
                     ATCSTARSAGGLRQQFSTPENIAMSQVMLDLVRNLKERFGPEADVSFHEQGYLLLASE
                     EGADVLAANVETQRAHGADIHLLDANGLKARFPWLATEGVACGSFGASGEGWLDPVSL
                     TTLFRTAARAAGVEYLHDTVTAMEVNGQAVETVQLASGRRIACGAVVNAAGPSGGIVA
                     ALAGIHLPVEPRKRYVYVVDCRDAPEELRRGPLTADASGVWFRPEGRLFLTGVSPDEE
                     NEPPAEDLDAIDYSLFQEEVWPRLATRVPVFESLKVVNAWAGFYDYNTLDQNAIIGPH
                     PEIRNFYFANGFSGHGLQQSAAAGRAIAELIAYGEFRTLDLTRFGYERIAERRPIREL
                     NVI"
     gene            15825..16703
                     /locus_tag="VF226_21300"
     CDS             15825..16703
                     /locus_tag="VF226_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX7076584.1"
                     /translation="MPVRPRRSALYMPGSNARALEKAKTLAADVVIFDLEDAVAPEAK
                     ASAREQVMAAVTGGGYGQRELVVRINGLDTPWGREDLARAAAAAPDAILVPKVSSAAQ
                     LAEVGRALASAPERVSVWAMIETTLGILNLREIAGAARDPATRLTCFVLGTNDLVKET
                     RAELDENRTAALYWLSAAVTAARAHGIDVLDGVYNSFKDIEGFRRECIQGRRLGMDGK
                     TLIHPDQIAPANEIFSPAAAEVEWARKVIAAFAEPQNQGKGVITVDGRMVELLHAEIA
                     RRTVEIAEAIAARENG"
     gene            16639..17784
                     /locus_tag="VF226_21305"
     CDS             16639..17784
                     /locus_tag="VF226_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7076585.1"
                     /translation="MLKSRGGRSRSRRRSPPAKTADTAIWEALMTSSKTNPGNFFEDF
                     RLGQEIHHATPRTLTEGDVAVYASLYGPRFAVQSSDMFARAIGYPRAPIDDLLVFHTV
                     FGKTVPDISLNAVANLGYADCRFLAPVYPGDTLSAQSEVIGLKENSSRETGVVYVRSK
                     GLNQDGETVLEYVRWVMVRKRDKSSPAPQAHVPDLPERVAPDALGHALPLLARADYDM
                     QMAGSPFLWGDYEPGERIDHSDGITIEEAEHQLATRLYQNTARVHFNQHTESAGRFGR
                     RLIYGGVVISMARALSFNGLANAYHIAAINGGRHVAPCFAGDTIYAWTEVLEKEELPH
                     RSDLGALRVRLVALKNRAASDFPYKTDLGDYEEGVVLDLDAWLLIPR"
     gene            18042..18458
                     /gene="sdhC"
                     /locus_tag="VF226_21310"
     CDS             18042..18458
                     /gene="sdhC"
                     /locus_tag="VF226_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEX7076586.1"
                     /translation="MAEAKARELPRIERPLSPHLQVFSPLINMVMSIVHRITGAALYF
                     GSLLLVWWLLAAASGPEQFATVNWFFGTFIGKIILFGYTWALIHHALGGIRHLIWDTG
                     LAFDLKSVDILCWGTIIGSVTLTVLIWAVVLLGGGA"
     gene            18463..18843
                     /gene="sdhD"
                     /locus_tag="VF226_21315"
     CDS             18463..18843
                     /gene="sdhD"
                     /locus_tag="VF226_21315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEX7076587.1"
                     /translation="MRTPLKNVRRLGSAKDGTDHFWRQRLTAVGALLLLPFMLWFAIT
                     LFGADYQTVRQTLASPIVAILLVVFIVTSAVHMRLGMQVIIEDYVHTDGTKIALLMLN
                     TFFAVLIGLAGTYAVLKMSFGVAM"
     gene            18843..20693
                     /gene="sdhA"
                     /locus_tag="VF226_21320"
     CDS             18843..20693
                     /gene="sdhA"
                     /locus_tag="VF226_21320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX7076588.1"
                     /translation="MADASGKRGESGVAKPAVTGRAYPLTDHTFDVVVVGAGGAGLRA
                     TLGAAQAGLKTACVTKVFPTRSHTVAAQGGVAAALGNMGPDDWRWHMYDTVKGSDWLG
                     DQDAIEYLCRNAPEAVYELEHFGVPFSRTEDGRIYQRPFGGMTTEFGEGPPAQRTCAA
                     ADRTGHAMLHTLYGQSLRYSAEFFVEYFAIDLLMNQEGACCGVVALSLEDGTVHRFRA
                     KKTILATGGYGRAYFSCTSAHTCTGDGNAMVLRAGLPLQDMEFVQFHPTGVYGAGVLI
                     TEGARGEGGYLTNSEGERFMERYAPHAKDLASRDVVSRSMTIEIREGRGVGPNKDHIY
                     LHLDHLDPKILAERLPGITESAKIFAGVDLRRQPIPVLPTVHYNMGGIPTNYHGEVLT
                     LKDGNPDYVVPGLMAIGEAACVSVHGANRLGSNSLIDLVVFGRAAGLRCAETVEPGGA
                     LPDLPADAADLALSRLDRFRHASGSTPTANLRLQMQKTMQNYCAVFRDGPVLAEGVEK
                     ITEVWKLSEDIKVSDRSLIWNSDLIETLEYDNLIAQAAVTVVAAENRKESRGAHARED
                     YPNRDDVNWMKHTLVWADYDTKTVRIDYRPVHNYTLTNEVSYIEPKARVY"
     gene            20929..21516
                     /locus_tag="VF226_21325"
     CDS             20929..21516
                     /locus_tag="VF226_21325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076589.1"
                     /translation="MKAASKPGDDTRLTPDPFSAVLPALAALGALTSIATVNWVAQER
                     TSERTGVRRKANAVLRDLESCCVGLQEIFRRFERHPKLFAGEGKAAASPLKFGVHGLR
                     ITPDEARLYQKSMDDIASMLVLATQNSFDVMSAIEDGEIDAPEELFFGFGEQQERLNG
                     LLTQRATLKTCVETGLSVAEQLTALVRGLRRHRVE"
     gene            21646..22425
                     /locus_tag="VF226_21330"
     CDS             21646..22425
                     /locus_tag="VF226_21330"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7076590.1"
                     /translation="MVQLRLPKNSTIEQGKTWNRPSGKGDWKEIKVYRWNPDDSHNPR
                     MDTYWIDRETCGPMVLDALIKIKNEIDATLTFRRSCREGVCGSCAMNIDGINTLACTK
                     ALDDVKGAVSIYPLPHMRVVKDLVPDLTNFYAQHRSIEPWLKTSSPTPGEEWRQSRED
                     RAKLDGLYECILCACCSAACPSYWWNSDRYLGPAILLQAYRWVIDSRDEATGERLDNL
                     EDPFRLYRCHTILNCAKTCPKGLNPAKAIAELKKLMVERRV"
     gene            23243..23416
                     /locus_tag="VF226_21335"
     CDS             23243..23416
                     /locus_tag="VF226_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076591.1"
                     /translation="MAELKTKVSKEEALYQDEPRAGEKCEACKHFIPEQSACEIVAGR
                     ISPQGWSKYFEPA"
     gene            complement(23462..24037)
                     /locus_tag="VF226_21340"
     CDS             complement(23462..24037)
                     /locus_tag="VF226_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7076592.1"
                     /translation="MSDHQIWGGAIRKLWPTEGDKFRDHLLRLDAESRRMRFAHAVSD
                     AFIVDYASRVHHMGSIIYCFLDGGDVRAAAELRKLSAVWGEEAEAAFSVERPWQDKGI
                     GTELMGRVIRAARNRGIKRLYLSCLAENGKMQAIARKYDAALRFEYGDVIGEIVPNGP
                     DYFSILAEEMDDRMGLMMAVLDLQNWRVKAA"
     gene            24240..25364
                     /gene="zapE"
                     /locus_tag="VF226_21345"
     CDS             24240..25364
                     /gene="zapE"
                     /locus_tag="VF226_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEX7076593.1"
                     /translation="MLKGPLESYGALITAGGIEPDAAQRHAAERLNALAQALARWRPK
                     RSLFSLFAGASSPPPRGLYIHGAVGRGKTMLMDLFHASVAFEPKRRVHFHDFMADVHE
                     RIAQARKSEPGDPIPAVARRIADEAKLLCFDELHVTDIADAMILGRLFKVLFESGVVV
                     VATSNSPPQELYRNGLNRQLFLPFVDLIEDHMDVLELAAAKDFRLEKLNGQQLYFTPA
                     DDAARAQLDAIWLSLTGNARGERKDLVIKGRTLRVPQAAMGVARFSFSDLCEKPLGSL
                     DYLHLARTFHTLLIERIPILGPQRRNEARRFINLIDTLYDNRVCLVASAEAEPDQLYV
                     SGDGADLFERTASRLMEMRSEAYLMDRNSRRAREQVSFGS"
     gene            25548..>26231
                     /locus_tag="VF226_21350"
     CDS             25548..>26231
                     /locus_tag="VF226_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7076594.1"
                     /translation="MARNKIALIGAGMIGGTLAHLIGLKELGDVVLFDIMEGIPQGKA
                     LDIAQAGGVEDFDCVLKGTNSYADIVGAQVCIVTAGVPRKPGMSRDDLLEINLKVMQQ
                     VGEGIKQYAPDAFVIVITNPLDAMVWVMQQKSGLPANKVVGMAGVLDSSRFRLFLAQE
                     FGVSVEDVTAFVLGGHGDSMVPLTRYSTVAGIPVPDLIKMGWTTQEKIDAIVARTANG
                     GGEIVKLLER"
BASE COUNT         4895 a         8285 c         8216 g         4835 t
ORIGIN      
        1 gctggccgac ggaatggtgg tccgcgcacc tgcgccgcct ccgccgcctc ctgttgtcga
       61 ccgctgcgcg ccaggtccgt ggacgggctt ctatgtcggc ggcaatctgg gctgggccca
      121 cctggatggc gagttcaacg atcacgacca cttcttctcg gaccgcaggt ggttcgatga
      181 tgacgaggac gcgttcaccg ctggcgtgca gaccggctac aacctgcagt gcgggaacgt
      241 cgttttcggt gtcgagggcg acatcaactg ggccggcttc gacgatgaca acgatcattt
      301 cggccacttc agagatggca agcttaagcg cgagcggttc aagcgcgaca ggtcaatgga
      361 ctggttcggc accatccggg gccggcttgg ttgggcgagc gaccgaatca tggtctatgc
      421 gactgccggc gcggcatata cggagctgga ccgcgggcac cggggtccgg ggcgcgattg
      481 gaatagtgac ctcagcatct ggggttggaa cgatcaccac gacgactcag acgatgtaaa
      541 ttggggctgg acagctggtg gcggtgtcga gtggctgtgg tcacccaatc tcagcttcaa
      601 ggcagaggcg ctttggatcg acttcgagga cggagacaac gaccgggata tcaagttcca
      661 cgccaaccac aaaggcactt tggtgacctg gcacgacagg aagcgttttg atcacgatga
      721 cgacatggtc gtcgtgcggg tcggcctgaa ctacaagttc gggtatcgcg ctccgccgcc
      781 ctacgagccc ctgaagtaac cctctcagtc cccgagaaca caaaggccgc ctcgctcgag
      841 gcggccttct tcatatttaa tcggttccgg cgatcactct gccgcgatca ctaccggcct
      901 gccggaaagt cccagttgtc gcttcgccat ctcacgaagt gcgtcgatcg gcttcagctg
      961 accctctgct tcaaaatgcc agaaggtcca gccgttgcag gcacttttcc cctgcacggc
     1021 agcgccgagg cgatggatcg agccctgctc accctgccaa gccagggtgc cgtcggccct
     1081 gacctcggcc ctcactctcc gacgttcgtc aaagagtagc gagccgggct cgagaattcc
     1141 aagctcgacg atggtgccga acggcacgcg cggttccgtt ctcttcgagc gcgccgtctc
     1201 gagtgcggtg ggactgacgg ggcggacccg ctcgatgcgc tcgctggcgg ctcgcgcgta
     1261 ctcagggtcg cgctcgatac cgatccaccg gcggccgagc cgcttggcca cggcacccgt
     1321 cgtaccggta ccgaagaacg gatcgagcac cacgtcaccg ggattggtcg tggcgagcag
     1381 aatgcgatgc agcagcgcct cgggcttttg cgtcggatgg gcctttcgcc cactggcgtc
     1441 cttcagccgc tccggccccg cgcagatcgg gaacagccag tcggagcgca tctgcaggtc
     1501 gtcgttgccg gccttcatgg cctcgtagtt gaacgtcacc cgggatttct gatccctccc
     1561 ggcccagatc aatgtctcgt gcgcgttggt gaaccgcttg ccgcggaagt tcggcatcgg
     1621 gttcgtcttg cgccagatga tgtcgttctg gatccagaag ccgagatcct gcagggccgc
     1681 gccgagccgg aaaatgttgt gataggagcc gatgacccag atggcgccgt cgcgtttcag
     1741 cacccgccgg cactcggcca gccaggcgcg cgaaaaccgg tcgtactcgg cgaaatcgga
     1801 aaacttgtcc cagtcgtcat cgacgccgtc gacccgcgtg ttgttgggac gcagcagatc
     1861 gccatcgagc tgaaggttat agggcggatc ggcgaaaacg agatcgacgc tggccgtcgg
     1921 cagcttggac agttcctcca ggcaatctcc gacgatgatg caatcaagat cgaagccggg
     1981 gcgtgcccgg tgacgcaaca ccatgactca actcgcacaa aacaacaccg cacgcaggca
     2041 aagccaactt aaagcccgcg gagacatcct caggagacaa ggttaacagg cggttaaggc
     2101 gagttcacgg ctgatccgag atcggatcaa acccgggtag cagctgcgcg acctccagct
     2161 cgtccggcgg aacgaggccg agcgcgacct ggacggggcg aaacgagcgg cggtggtgct
     2221 tcgttacgcc gaggcggttg atcgccgcga agtgctccgg ggtcgaatag cccttgtggc
     2281 gctcgaagcc gtagccggga aatgcccggc ccaactcgac catcatgcga tcacgagtca
     2341 ccttcgcgat gatggacgcc gcggcgatcg aaaggcattt ggcgtcaccc tctacgatgc
     2401 attgcgtatc gcaggagagc ttgggcggcc ggttgccatc gacgagcgca acgcgcggct
     2461 tcggatcaag ctgccggaag gcttgccgca tcgcccagag cgtcgcgttg aggatgttgt
     2521 atctgtcgat gcgggcaacg ctggcaacgc caatacctac tctcgacgtt gccatgatct
     2581 gagcgaacag ctcttcgcga acctctggtt ccaatgtctt ggaatcggca atgccggaag
     2641 gaatgcgttc gggatcgagg atcactgcgg cagcgacgac gggaccggca agcggaccgc
     2701 gcccggcctc atcaatgcct gccacgggcc ctccggtcag ccgtatcgac acggcttcca
     2761 cctcgaacgt cgccctcggc tttgaatcgt cccccgccat gagccaagct tctacggctc
     2821 aacgcggcgg tgtcaaagga gttgcagctg ccctttgtaa ctgcctgtgg gttttcggaa
     2881 caggtccgtg cgaagcttca gtccgcgctc attcatgcca agccgtctga gagcaagccg
     2941 aaaacgggtg gcgatctgat cggcataggg cccgctgccc cgctggcgga tgccgaattg
     3001 cgatgtgtaa tcccggccac catgcatcga ctgcaagacc ccgatcaccc gctttgcgcg
     3061 gtcggggacc tccgttgcca gccattcgcg gaaaagctcc ttgagctcca agggcagccg
     3121 caacagcacg tagccggcat cccgcgcgcc ggcctcatgg gcggcttgca ggatgcgctc
     3181 gatttcgctg tcattgaggg ccggcacgat gggggcgacc atcacctggg tcggaatgcc
     3241 tgcgctgctg agctgccgta tagcctccag ccgcctcgct ggagtgctgg cccgcggctc
     3301 catcttgcgc gcgacgtagc ggtcgagcgt cgtgaccgac aatgcgacct tgacgagccc
     3361 gcgctcggcc ataggagcca ggatatcgat atcccgcgtc acgagagccg acttggtgac
     3421 gatgccgacc gggtggttgg tcctgtcgag gacctccagg atggaccgcg taatgcagta
     3481 ctcgcgctcg atgggctggt agggatccgt cacggcgccc agcgcgatcg tgctcggccg
     3541 gtagccggga cgcgacagct ccttttcgag cagctcggcg gcattcgttt tggcgtagag
     3601 cttcgtttcg aagtcgagcc ccgccgaatg cccgagatag cagtgggtcg gtcgggcgta
     3661 gcagtaaatg cagccgtgct cgcacccacg ataagggttt atggagcggt cgaaaccgat
     3721 gtcgggactg tcgttgctgg caatgatgct gcgggcaacc tcgatccgca cctgggtctt
     3781 gaagggctcc agctcggcca ggctttccca gccgtcgtcg aattcctcgc gaagctcggt
     3841 ttcgtatcgg cccacgcgat tggagcgcgc accacgccct cggcgccgat cggcgtcaac
     3901 gagctgagct gcagccggat gagcggggtt gttgggagca gccgacattt acgcatcgtc
     3961 caacggaacg gtgtcttgga acgcgactat atccaaccaa tcgaacaaag caagaacaaa
     4021 gccgtgcttt ggcggcagcg ggctcccggg ctatcaaaga actcccatga tttcggtcat
     4081 catcccgacg ctgaatgcag aggccacgct tgcgtccaca ttggcgtccc tcgtgccggc
     4141 ggcggtggac ggggtggttc ggcaggtcat cgttgccgat ggaggatcga ccgatcggac
     4201 cctcgaaatc gccgatgcag cgggcgcaga catcgtgcaa ggggaagcgg ggcgtggtcg
     4261 ccagctggcg cggggagcaa aagcagcgcg ctcggcatgg ctgctcttcc tgcacgcaga
     4321 cacggtgctc gagcccggct ggcacacgga agcagcagcg ttcatggacg gcatcgaaac
     4381 ggatcatcgc cctgtttcgg ctgcagcatt ccgcttcacg ctcgatgatg caggaatggc
     4441 acctcggctc attgaagccg ccgtgtggct gcgttgcgcg ctggccagac ttccttacgg
     4501 cgaccaggga ttgctcctcc cggctcggct ttatcgggag atcggcggct ttcgtcccat
     4561 gcccatcatg gaggatgtcg acatcgtccg gcggcttggt tggagacgtt tgacaatgct
     4621 cagctcgcgc gctgttacga gcgcgtcacg ctaccgccgg gacggatatg gccgacgaac
     4681 agcgcgcaac ctcagctgcc ttgctcttta cgcgctgagg gtcccgccgg acaccattgc
     4741 ccggctatac cgatagatca cgcgccttca aaactgcgtg agacggcatc gacgacttct
     4801 cctcgcctgc ataggtgcgt acgctcctat tatcgtgatc caagtaaatg cgcggtcagt
     4861 tcgtttaagg agcagccatg accctgttgt ctcgccgcag tttcgtcgtc tcggccgcga
     4921 ccgcttcagc agccttcggc ctcaaaggcc ggcttgcctt cataaatccg gtccaagccc
     4981 agagcaccga tctcccgagg ttcgcgcggt tcgcaatcgg cgatatcgag gtaacgcagc
     5041 tctatgacgg cgtttggcat aaggcgcacg acccgaactt catcgcgggt gtcagcgtgg
     5101 aggaaaccaa gaaggcgcta cgggatgcaa acctgactga tgcgcacgtg ccgatcacct
     5161 tcacgatcac cgtgctccgg atgggcgaca agctggtgat gttcgactcc gggactggcg
     5221 gacagatggc gcctacggcc ggcctgctga tgaaggaaag catgcaggcc gccggcatcg
     5281 acccgaagag catctcgacc atcatcatga cgcacttcca tcccgaccac attttcggcc
     5341 tgatggagaa gggtacgaac gcgcaggtct tccccgatgc ggagctcgtg gtgcctgcga
     5401 gcgagtacaa gtactggacg gatgaaggcg tgattgcaac tctgcccgaa gcccggcagg
     5461 gtctggcgca gcgcattcag gccaccttcc ccaaatggaa gaacgtcagg caggtcgaaa
     5521 acgatgccga agtcgtacca ggggtccgct ccgtcgcgag caatggccat acacccggcc
     5581 acacgagcta tctggtgagc tccggaagcg atcagctcat ggttctggga gatgtgacca
     5641 acctccccgc cctgttcgtc cgcaaccccg gctggcatgc ctcgtttgac caggaccctg
     5701 agatggcgga gagcacgcgg cggaagatgt tcgaccgggt gatcgccgac aaaatcacca
     5761 tcaccggcta ccactatggc atgccgggcg ccggcaccat cgaaaaggac ggtagcggct
     5821 acgcctacaa gccaatttcc gcctgacttc gggccggcct gaccccgcat gcgccgctcg
     5881 agaatgttgc ctgcagaggc gccgcgaaga ccagcggcgc cttttcgctg ccggttggtg
     5941 atcatggcga aactgccggt ggccggtagc gtgaagacga ggctggcgcg cgaaattggt
     6001 acggtgcgtg caacgtggtt ctaccggcac gctgcagcag ccgtgatcgc acggatggcg
     6061 cgatcgagga tctgggaaac cgtactggcc ataacgcctg attcctcagt cgctcatccg
     6121 acttggccgc gcggtctgac gcgaatgggg cagggtagag gagatctggg gcggcgcatg
     6181 caacgggcga tccgtctgca gccgcccggc cccgtcgtca tcatcggtac tgacgttccg
     6241 ggcattcgcc cctcggacgt cgccgcggct ttcaagtcac ttggcagcca tgacgccgtc
     6301 tttggcccgg catccgacgg cggctattgg ctcgtcgggc tgaggcgccg gccacgcgag
     6361 atcgccatgt tcaaggacgt gcgctggtcg agcccccatg ccctccagga tacattgcgc
     6421 aacctgcgct gctatcgcat tggctttgtt gccgaaaaaa gcgacgtaga cgacgcagcc
     6481 gacctgcgcc gtgtcaccac ttgttttggt cgccgagtcc tccccgcact tgctgacgag
     6541 ctcacctctc cgaaaaacct ctgaatcatt gcagattctc tgttgacgtg tcttattatg
     6601 gtcacgggcg gagtaagggg ttgtaggatg gtacaagcat cgccgcaggt gaccgcggcg
     6661 caggtcctcg atgcgggccg gcgcgccgaa gcggatggac gcgtagagta cgccgtccag
     6721 ttctatcgcc atttgaccga tcatcactct ggtgcgccgg aagccgccgc ggctcgggag
     6781 gcgctttccc ggctcaagcg gcagggcaat ctttccgatc aagtatcacg aatgcggccc
     6841 gcaaatcctc cgccgctgcg cagcgatcat cgcggcgccg atgaaatgaa gcgagttggc
     6901 cccaaggtcg ggcgcggtcc gatccgcatt gcgccggtcg gggccgggca ggcgccggtg
     6961 agaatggagc tgcccgtgcc gccagcggac tacttcactg gacggctgat tgcgtacgcc
     7021 ttcgccggag ttggtgggct gctcttcctg gcaggattac tactggccat cgcggttgct
     7081 ctaatgccgg ctgaagtcgc tgcccgcatc cccgcatgga tgccggtgtt tcatccactc
     7141 accgggacag gcgccgcgat gcttggcctt gttctcgtgt tctcgggaca ggttggccgc
     7201 gccctcttcg acctcgccat tgcgagccgg gacatcgcag ctatcgagcg ggccaagctg
     7261 gagcacgcca tcgcggaggc ccgctaaggt caggcgccag ctttcttccg gtgctcaccg
     7321 aggcgcggca tgatctcgac gaggttgcaa ggacggtgcc ggttgtcgag ctggtactgc
     7381 aaaatcccgt tccacgcatc gcggcaggcg ccgggcgagc ccggaaggca gaagacgtag
     7441 gtctccccga tcagaccggc gcatgctcgg gactggatgg tcgacgtgcc gaccttctcg
     7501 tatgagatca aatgaaagac ggtcgaaaag ccgtcgatct ccttctcgaa gagcggcttt
     7561 agagcgtccg gagtgacgtc gcgcccggtg aagccggtcc cgcccgtcgt aatcaccacg
     7621 tcgatgtccg gatcggcgac ccaccgcagc acctgggcgc ggatctgtgg aatgtcgtcc
     7681 ctgacgagcg cgcgggccgc gagcttgtga ccagccttct cgatcagctc ggccaaggtc
     7741 gccccggact tatcgtcctc gagcgcgcgg gtgtcggaca tcgtgaggac ggcgatggag
     7801 accgggatga acgggcgcgt ctcgtcgatt ctggccatgg ctcgaacagc tcctttgcag
     7861 agccagtcta cgccgatccc tcgtccgctg catcgggcga attgccctcg cccagccgtg
     7921 cacgcaaagc gagcaggtcg cgccaggcaa gtcgtttcga ggctggcgta cgaagcaggt
     7981 aggccgggtg cagcgtcgcc atcactggga ttctccggct gccgatctcc acctcacgcc
     8041 acttgccccg gatgcggaga atgccttccg tgccgccgag gatgtgcctc gtcgcggctg
     8101 ctcccagcag caaaaggaag tccggccgga ccagctcgat ctgccgcacc agaaatgggc
     8161 gacacacgat cgcctcctga ggagtggggg tgcggttacc gggcggccgc caatagacga
     8221 tgttggtgat gtgcgtgtcg gcctcgctaa ggccgatcgc gttgagcata cgatcgagga
     8281 gctgcccggc gcggccgacg aatggcttgc cttcgaggtc ctcctcccgg cccggcgctt
     8341 cgccgatgat catcacgcgg gcctggggcg cgccgcggta gaagcacaag ttcttggccg
     8401 tctgccgcag cgcgcaccct tcgaacgtct cgagcgccgc ccgcagctcg tcgagtgacc
     8461 tggcagatcc cgctgcggct ttggccgagt cttcagcctc ggtcgcgggc accgtcgcaa
     8521 agctgcgggc aggaactgcc gtcggcccac gtgaggggcc tcgcgagggg atctggaagc
     8581 tctccgaaaa ggtggtttcg ggtgctggca actggctcac tgacctggca gcagccggct
     8641 ctgccggttc gggcgtgagc acggcagcat cccctcgcgc gaaccagttc agcggctcgt
     8701 cgctgagcgc gtctgtcacg cccgcctcgc gataccacgc gaggagcgcg atcaggcggg
     8761 gatctgggga atgctccatg gctgcaatct agcccaaacc ggcacgaatt cgagggttct
     8821 tgtcgcgcag cgatcgaatt taccgccaaa cccttgacgt agcgaataaa caagggccgt
     8881 aatcgcgccc gtcgaggcat ttgctgggat accttgaagc atggcacgcg agccagagca
     8941 gctaccgccg cgcgaggcga tggacttcga tgtcgtgatc gtgggggccg ggccggccgg
     9001 tttggcggcg gctatccgtc tgaagcaact cgcgctcgcc gacggcaacg agatttcggt
     9061 cgtcgtgctg gaaaagggct cggaggtcgg cgcccatatc ctctctggcg cggtgatcga
     9121 tccggtcggc ctcgacgcct tgatccctga ctggaaggag cgcggtgcgc cggtcgacac
     9181 gccggtgacc gaggaccgct tcctttatct cggccctgcg ggcgagttga agctgcccaa
     9241 cttcctcatg ccgccgctga tgaacaacca cggcaattac atcgtcagcc tcggcaatct
     9301 cgtgcgctgg cttggtgagc aggctgtcga gctgggcgtc gaagtctatc cgggtttcgc
     9361 tgcaaccgaa gtactgcggg acgagagcgg cgcggtaatc ggtgtggcca cgggggacat
     9421 gggcgtcggc cgggacggca agcccaagga caacttcacc cgcggcatgg agctaagggg
     9481 caagtacacg ctcattgccg aaggcgcaca cggctctctc gccaagcagc tcatcaagga
     9541 cttcaatttg cgggaaggcc gggagccgca gaaatacggc atcggcatca aggagctttg
     9601 ggaggtcagc ccggagcggc atcggcccgg cctcgtgcag cacaccttcg gctggccgct
     9661 cgacaatcgc acgggcggcg gatcgtttct ctaccactac ggggagaatc tggtcgcggt
     9721 tggcttcgtc gttcacctga actacgagaa tccatatctg tcgccctacc acgagttcca
     9781 gcgcttcaaa actcaccccg tcatccgccc gcacctcgaa ggcggcaagc ggatcggtta
     9841 cggagcgcga gccatcacgg aaggcggctg gcaatccctg ccgaagctcg tcttccctgg
     9901 cggcgcgctg ctggggtgct ccgccggcat ggtgaacgtg ccgcgcatca agggcagcca
     9961 caatgcgacc ctgtccggca tgcatgcggc ggacgccgtc tcggatgcgt tgaagcatgg
    10021 ccgctcccac gacacgctgg acgcctacga gcgtctggtc cgtacgggac cgatcgcgcg
    10081 cgacctgcgg ccggtgcgca acgtcaagcc gatgtggtcg cggttcggat cgtttgccgg
    10141 catcgcgctc ggcggcatag acatgtggct gaatacgatc ctgccgggcg tcggcctcgg
    10201 ctacacgctc cgccatggca agccggacta cgcgaccctg aacaaggctg cgaaagcgaa
    10261 gcccatcgat taccccaagc cggatggcgt gctcacattc gacaggctca ccaatgttgc
    10321 tttctcgggg accaatcacg aagaggatca accggtgcac ctggtcctgc aggacccgag
    10381 cattccgatt tccgtgaacc tgccggaata cgacgagccg gcccagcgct actgcccggc
    10441 cgcggtttac gaggtggtca tggacgagga cggcaggccg cggttccaga tcaacgcgca
    10501 gaactgcgtg cactgcaaga cctgcgatat caaggacccc agccagaaca tcaactgggt
    10561 gcctcccgaa ggcggcggcg ggcccaacta tcagggaatg taaagccgac gcgaaaaccc
    10621 ggctcgggaa tcgcctgatt ccgtcaagcc gaagggtgat gagcgctcgc ggcggcatcc
    10681 gatggggaca gcggcaggcg gatcgcgcac gtcacgcctt cggggtcgta ggagatccgc
    10741 gacgagccgc cttgggcctg ctgcaacgag cgctcgatca gcagagtacc gaagccggtc
    10801 gacgccggtg gggtgacggg tggtccgccc cgttccctcc aggtgagatg gagaacggac
    10861 gatccgcccg cctgctccaa tgtccagtgc acgtccaccg tgccctgcgg cacggacagc
    10921 gagccgtatt tccgcgcgtt ggtgccgagc tcgtgcagaa ccagcgcgag ttgaagcgcc
    10981 gtctgcggat cgagcgcgac ctgcgggcct tcgcaggaga tgcggtctgc actgcccagc
    11041 atcaattgct cgcggatgag agcatcgagc tcggtcccct gccaggccgt tcgggtcagt
    11101 agcgtatggg cgcgggacag cgcctgaatg cgctcgctga agcttgggac gaagtcgtcg
    11161 gggcttttgg cccgcctcag cgtttggctg gcgatggcct gcacagtggc gagcgtattc
    11221 ctcacccggt gattgagctc gccgacgagg agctgttgcc tccgctcggc ctccttgcgc
    11281 tccgtgatgt ctgcgatgac tccgaagatg gcctgcggat ttccctgggc atcgtagcgc
    11341 acgaggccgc ggctatgaat ccagatgtac ctcccatcct gatgacggac acgcgcctcg
    11401 agctccaatg agccgctcaa cttcgccgct tcgaggttct gccaaatgag ctcgcggtca
    11461 tctgggtgga tgagggccga ggtgttttcg attgtcggct cgaacgtcgc cttgtcgagg
    11521 ccgtagatcg cgtagacatg gtccgaccaa ctgcagcggt tggtggcgaa atccaattcg
    11581 tacgtgccca tgcgtcccgc ggcgagcgcc agggacgttc ttgccatggc ttccctgagc
    11641 tgccgcgccg ccctgtcccg ctcggtcacg tcatgcgcga cgacagcata gccgatcgtc
    11701 cggccgacga gctcccgaat gggcgtcaat gaaagaatga cgcggatctt ggagccgtcc
    11761 tttcgccgac gggtcgcgtc caccgtcacc gcggcaccgc tctcctgaac gcggcggaca
    11821 aggtcatcca gcgctggaag ctccgagcgc gggcacaggt cgcgctcaga ctttccgatg
    11881 atctcagacg gtgaatagcc gaagatcgtc tccgcgccgc gattccaggt ctcgatctgc
    11941 aggctcggcg agagcacata gatcgcatcc ttcgaggcct ggacgacggc ctcgtggcgc
    12001 agacgcagca catggcggct gaggtcggtg acgaccaggc cgtagagggt ctgaccatcg
    12061 atcgtcagcg atctggtccg cgagatctgg acgggaacgg atgtgccgga aagcgtacgc
    12121 aagctcgttt gcatgctgtc gctgtccgaa agcacgagcc gctgcaggaa atggctcggc
    12181 tcacccaaga cgaactcgcc gaaactacgg cccagaatac ggttcttcgg taagcctagg
    12241 agccgggcca ctgcctcatt gcagtaacgg atcgcaccgt cggccgtcag catcagtacg
    12301 ccgtcgctca tgtgctcgac aaggagccga aatgcctcga gcgaagactg gtgatctgcc
    12361 gggagagcat cggttggcca tggcgcctgc ccaccggtgc ccttcttgta gcttgcgagc
    12421 gccgactcgg ctttggccag ctgctggcgc agcacgtgca cggctcgttc cagctccagc
    12481 gtccgatcgc cggtatgcct catatcggga tcacaggtgc acatcggctc ggcttcgctt
    12541 ctcccaggct taccgaacat tttcactttc cgcgctcgca gaagggatcc tccagtccgc
    12601 cactgatcaa gcgcaggcca gcaggccact gcgtatggat actggcgctg acatcgatga
    12661 gatcaggcga aaatcttcat ctcgataggt gacaagatta tgccccttac tcagctcccc
    12721 tgctcgaata cttaggcaaa aacacgaccg ctgtggcgat atgctatacc gcatctgacc
    12781 gcgatcgacc gctcggcatg aactaatctt aacgtgacct gggtcgaact gaacatgcct
    12841 taacgcaaat ctggtcatca tcagggagct gcgacatatt gcacctcccg accgatctcc
    12901 cccgccggaa catgtcagat cgagacgaac tcgcccgtac cctggttcaa caccgcaagc
    12961 tgccctgtcg cgatctcgaa atgcgcacca tgcagatgaa gacggccctt gctttccagg
    13021 ctcttgatgc acggaaagct gcgcaggttc tgcagtgatg tcttgatgcc aaggcgctcg
    13081 agacgggagc gcaactccgg aatgctttga tctccacgcg ctgcgagcac tttgcggcgc
    13141 gcgtcctcca gaatagccat ccaattcgaa atgaactgcg cttccgactg acgggccgca
    13201 gactcctcaa gacaggcgct gatgccgccg cagcccgaat gcccgagcac gacgatgtgc
    13261 ttcacgcgca gattgagagc ggcaaactcg agcgccgcac tgacaccatg atatgtgccc
    13321 gaggtctcga acggcggcac gaggttggcg acattgcgca ccacgaacag ctcgcccggc
    13381 atcgcgctga acagcgtctc tggatccacg cggctgtcgc agcagctcac caccatcaca
    13441 tccggatgct gaccatattg ggccagctct tcgtagcggt cgaaattggg cgcgaaatgc
    13501 cgaaacttga agcgacggaa ccggtctgcc aagtgctcgg gaaattggct catgacgatc
    13561 cgccccttca aggttgatca ggcttgtgcg gcgataatca gtatgagcgc cggcttcagc
    13621 gttcaactgc cgccggagct tcatcctgca tgccattgcg agacttcaag cggtttggcg
    13681 gcaacccagg cctcctgaga agccaccagc gccagttcgt cgcggccggc gctcgccaga
    13741 atggcctgct ccacgcccgc cgcagcaaag gccatcgctg atgtctcggt cgccccattc
    13801 aggtgatgcc ccaggacgag ggctgaaaag agatcacccg tcccatgggg gacggattcg
    13861 cggtaaggca cctcgcaggc tttcacccca tcctcgtcga tcagcaggtt ctcgagcatt
    13921 gggcccctgc gcctcgcgga ggtcaccagc actgtcgcgg gcgccagtcc ccgcgcggca
    13981 ttgggtagaa cgtcatccgt ctttatcggg atatccgaca gccagctcag ctcgaaccgg
    14041 tttggagtga tgatgtctgc taagggaacc agttcgtcac gaatggccgc tgcggcggca
    14101 gcctctatgt agaggccctt cggctcgtct ccgaggaccg gatcgcaaag gtacgttagc
    14161 ttgccgttag ccctgagccg ccgaatcacg cttgccgcca gacaggcatg ttcggcgctc
    14221 ggcagatagc cggtgatggc agcgtcgatt tcaccgagcc agccgttccg gtcgagcgca
    14281 tcgaccactt cttccagaac agcggtctcg acgcgcatcc ctgcggcgtg cgggtggccg
    14341 ggatggttcg aaagaataat gctcggcagc ggccacacct cgtgcccgag ccgctgcagc
    14401 gccggcacga ttgcggaaag gccaacatgc ccgcgaacca cctgcgacga gaaggcgagc
    14461 acgcgggcca ttcgagcctc attcagatta cattgagttc ccgtatcggc cggcgttcag
    14521 caattcgctc gtagccgaag cgggtcagat ccaatgtgcg gaactcgcca taggcgatca
    14581 actccgcgat cgcccggccg gcagccgccg actgctggag accatgtccg gagaagccat
    14641 tcgcaaagta gaagttacga atttccggat ggggcccgat gatcgcgttc tggtcgagcg
    14701 tgttgtaatc gtaaaatccc gcccaggcat tcacgacctt gagcgactcg aataccggta
    14761 cccgggttgc aagacgcggc cagacctcct cttggaacag gctgtaatcg atcgcgtcga
    14821 ggtcctccgc tggtggctcg ttctcctcat ccggcgagac tcccgtgagg aacagccggc
    14881 cttctgggcg gaaccagacc ccgctggcat ccgccgtgag agggcctcgc cgaagctcct
    14941 ccggcgcgtc ccggcaatca acgacataca cgtaccgctt gcgaggctcg accggcaggt
    15001 gaatgcccgc cagagcggcc acgatgccgc cggaagggcc ggcagcattg acgacagcac
    15061 cgcacgcgat gcgccggccg cttgccagct gaaccgtctc gaccgcctga ccgttcacct
    15121 ccattgcggt gactgtgtca tggaggtact ccacccctgc tgcgcgcgcg gccgtccgga
    15181 aaagcgtcgt gaggctcacc ggatcaagcc agccttcgcc ggatgcgccg aagctgccgc
    15241 aggccacccc ttcagtggcg agccaaggaa agcgcgcctt caagccgttt gcatcgagca
    15301 ggtgaatgtc ggcgccgtgg gcgcgctggg tctcgacatt cgccgcgaga acatccgcgc
    15361 cctcttcgga agcgagaagg agatagccct gctcgtgaaa gctgacatcg gcctccggac
    15421 cgaagcgctc cttcaaattg cgaaccaggt cgagcatgac ctgggacatg gcgatgtttt
    15481 ccggagtaga gaactgttgg cgcagcccgc cggcagaccg tgcggtgctg caggtggcga
    15541 aacttggatc cctctcgacg ataacgaccc ggcgctcgtc caagacgccc gtctgcgcca
    15601 agtagtaggc tgccgcacag ccgatgacgc tgccgccgac gatcacgatg tctgttgata
    15661 ggtccgccat accttctcca cacatccagc cgaactcgca ttgtgccgga gcgtgctccg
    15721 ggtgccaagc ggacttgccc gcgcaaaagg aaattctggc tttcaaaatc tgacgccgct
    15781 gcactagctt acggcttgga atagacatca cgcgagtaga agcgatgccc gttcgccccc
    15841 gccgtagtgc gctctatatg ccgggctcaa atgcccgcgc actggagaaa gcaaagacgc
    15901 tcgccgccga tgtggtcatc ttcgacctgg aggatgccgt cgcgcccgaa gcgaaggcat
    15961 ctgcgcgcga gcaggtcatg gctgccgtca cgggcggggg ctacggccaa cgtgagctgg
    16021 tggtgcggat caatggcctc gatacgccgt ggggtcgtga ggaccttgcc agggctgcag
    16081 cggccgcacc ggatgccatt ctggtgccga aggtttcgtc cgcggcgcag ctcgcggagg
    16141 tcgggagggc tctcgcctcg gcacccgaga gggtcagcgt atgggcgatg atcgagacga
    16201 cgctcggcat tctcaatctg cgggagatcg ccggcgccgc ccgcgatccg gcgacacgct
    16261 tgacctgctt cgtcctgggc accaatgacc tggtcaagga gacccgggcg gagctcgacg
    16321 agaaccggac cgctgcgctc tactggctgt ccgccgcagt caccgcggcg cgcgctcatg
    16381 gaatcgacgt cctcgacggc gtctacaatt ccttcaagga catcgagggc ttccgccggg
    16441 aatgcatcca gggccgccgg ctcggcatgg acggcaagac gctgatccac cccgatcaga
    16501 ttgcgccggc caacgaaatc ttttccccgg cggccgccga ggtggagtgg gctcggaagg
    16561 tcatcgcggc attcgcagag ccgcaaaacc agggcaaggg cgtcatcacc gtcgatggcc
    16621 gcatggtcga gctgctgcat gctgaaatcg cgcggcggac ggtcgagatc gcggaggcga
    16681 tcgccgcccg cgaaaacggc tgacaccgcc atctgggaag ctctcatgac atcaagcaag
    16741 acgaaccccg gcaacttctt cgaggacttc cggctcggcc aggagatcca tcacgcgacc
    16801 ccgcgcacgc tgaccgaagg ggatgttgcc gtttacgcca gtctctacgg cccgcgtttc
    16861 gccgtgcaat cgtccgacat gttcgcccgg gcgatcggct atccgcgcgc acccatcgac
    16921 gacctgctcg tgttccacac cgtattcggc aagacggtgc cggacatctc gctcaatgcc
    16981 gtcgccaatc ttgggtatgc agactgccgc tttctggcgc cggtctatcc gggggatact
    17041 ctgagcgccc aatccgaggt gatcgggctg aaggagaact cgagccgcga gacgggtgtc
    17101 gtatacgtcc gttcaaaagg gctcaatcag gatggcgaga cggtcctcga atacgtccgc
    17161 tgggtcatgg tgcgcaagcg ggacaaatct tcaccggcgc cgcaagcgca cgtgcctgat
    17221 ctgcccgagc gcgtcgcgcc cgatgcgcta ggccacgcac tgccgctgct ggcgcgagcc
    17281 gactacgaca tgcagatggc gggctcgccg ttcctgtggg gggactatga accaggggag
    17341 cgcatcgacc actccgacgg gatcaccatc gaagaggcgg agcaccagct cgcgacgcgc
    17401 ctctaccaga acaccgccag ggtgcacttc aaccagcaca cggagagcgc gggtcgcttc
    17461 gggcggcgcc tgatctacgg cggcgtggtg atttcgatgg cccgtgcgct gtcgttcaac
    17521 ggtcttgcca acgcctatca catcgcagcg atcaacggcg gccggcacgt ggccccatgc
    17581 ttcgcgggcg acacgatcta cgcctggacg gaagttctgg aaaaggagga gctgccccat
    17641 cgcagcgatc tcggcgccct gcgcgtccga ctggtcgccc tcaagaaccg cgccgcaagc
    17701 gatttcccct acaaaaccga tttgggggac tacgaggagg gtgtggtgct cgatctcgac
    17761 gcctggctgc tcattccgcg ctagcaactc ggctcggaac caggacttgc ggcgcggttc
    17821 tgcgagcaaa ggacactcgg tcgccagcct tgatcgcccc tctcacgccg agaaattcgc
    17881 ttgacgtcgg acccgcgggc gctggcgaag ttccagatca tcacaccaga tcaggtattc
    17941 ccgtgccgcg gagctggcat gcgtccgttc gagtaattgc tgcggtggcg aacgcgcgct
    18001 acaagacccc ccgacgttat ctgcgccacg aggagagccc catggccgaa gcgaaggcac
    18061 gggagctgcc gcggatcgag cgaccgctct cgccgcacct gcaagtcttt tcgccgctca
    18121 tcaacatggt gatgtcgatc gtccaccgta tcaccggggc ggcgctctac tttgggagcc
    18181 ttttgctcgt ctggtggctg ctcgctgcgg cgagcggacc ggagcagttc gcgacggtga
    18241 actggttctt cggcaccttc atcggcaaga tcatcttgtt cggctatacg tgggccctca
    18301 ttcatcacgc acttggcggc atccggcacc tcatctggga caccggcctc gcgttcgatc
    18361 tgaagtccgt ggatatcctg tgttggggca ccatcatcgg ctcggtgaca ctcactgtgt
    18421 tgatctgggc tgtggtgctg ctgggcggag gggcatgaca cgatgcgtac accattgaag
    18481 aatgtccgcc ggctcggatc ggctaaggac ggcacagacc atttctggcg ccagcgcctc
    18541 acagctgtcg gagccctgct gctcctgccc ttcatgctgt ggttcgcgat cacgcttttc
    18601 ggcgccgact accaaacagt caggcaaacg ctggcgagcc cgatcgtggc gattctgctc
    18661 gtggtcttca tcgtgacgag cgccgtacat atgcggctcg gcatgcaggt gatcatcgag
    18721 gactacgtgc atacggacgg caccaagatc gccctgctga tgctgaacac gttttttgcg
    18781 gttctgattg gacttgcggg cacgtatgcg gtgctcaaga tgagctttgg ggtagcaatg
    18841 tgatggctga tgcgagcggc aagagaggtg aatccggggt tgccaaaccc gcagtgaccg
    18901 gacgggccta cccccttaca gaccacacgt tcgacgtcgt ggtggtcggc gcgggcggcg
    18961 caggcttaag ggccacgctc ggagcggcgc aagccgggtt gaagacggcc tgcgtgacga
    19021 aggtgtttcc aacccgctcc cacacggttg ccgcccaggg cggcgtcgcg gcggctctcg
    19081 gcaacatggg gccggacgac tggcgctggc acatgtacga caccgtgaag ggcagcgact
    19141 ggctcggcga ccaggacgcc atcgaatatc tctgccggaa tgccccggaa gcggtatacg
    19201 agctcgagca tttcggcgtg cccttcagcc ggacggagga cggccgcatc tatcagcgcc
    19261 cgttcggtgg catgacgacc gagttcggcg agggaccgcc ggcccagcgc acgtgcgcag
    19321 ccgccgaccg taccgggcac gcgatgctgc acacgctcta cggtcagtcg ctgcgctact
    19381 cggccgagtt tttcgtcgag tacttcgcca tcgatttgct gatgaaccag gaaggggcgt
    19441 gctgcggtgt cgtcgcgctg tccctcgaag acgggaccgt ccatcgcttc cgcgccaaaa
    19501 agaccattct ggcgaccggc ggttatggcc gtgcctactt ctcttgcact tcggcgcaca
    19561 cctgcaccgg cgacggcaac gcgatggtgc tgcgtgccgg cctgccgctg caggacatgg
    19621 aattcgtgca gttccacccg acaggcgtct acggcgccgg tgttctgatc accgaaggcg
    19681 cgcgcggcga gggcgggtac ctgacgaact ccgaaggcga gcgcttcatg gagcggtacg
    19741 cgccgcatgc caaggacctg gcctcgcgtg atgtcgtctc ccgctcgatg acgatcgaaa
    19801 tccgtgaggg ccgcggtgtc gggccgaaca aggatcacat ctatctccac ctcgatcatc
    19861 tcgacccgaa gatcctggcg gagcgcctgc ccggcatcac cgaaagcgcg aagatcttcg
    19921 ccggcgtgga tcttcgccgg caaccgatcc cggtgttgcc gacggtgcat tacaacatgg
    19981 gcggcatccc cacgaactat cacggcgagg tcctgaccct gaaggacggc aatccggatt
    20041 acgtggtgcc tggattgatg gccatcggcg aagcggcctg cgtgtcggtc cacggcgcca
    20101 atcgcctcgg atcgaactcg ctgatcgatc tcgttgtgtt cggacgtgcc gccggcctgc
    20161 gttgtgccga gacggtcgag ccgggcggag ccctgccgga tctgccagca gacgcagcag
    20221 atctggcgtt gtcacggctc gatcgcttcc gtcatgcatc gggctccacg ccgacggcaa
    20281 acctgcggct ccagatgcag aagacgatgc agaattactg cgccgttttc cgcgacgggc
    20341 cggtgctcgc cgaaggggtc gagaagatca ccgaagtctg gaagctgtcg gaagacatca
    20401 aggtcagcga tcgatcgctg atctggaact ccgacctcat cgagacgctc gagtacgaca
    20461 acctgattgc ccaggccgcc gttacggtcg tcgcggccga aaaccgaaag gaaagccgcg
    20521 gcgctcatgc gcgggaggat tacccgaacc gggacgatgt aaactggatg aagcacacgc
    20581 tggtctgggc ggactacgat accaagacgg tacggatcga ttaccggccg gtccacaact
    20641 acacgctcac gaacgaggtg agctacatcg aaccgaaggc tcgcgtgtac tgagggcgac
    20701 ccggcacgtt tcgaacagaa tggcggcttc ctgtcaggac gccgccattt ttcaagcgcc
    20761 tgtacttgcc gtgatggtgt caccgcgcgg ccgtcgtggg cgcttcagcc gatcagcacg
    20821 ctttccgcgc gcctccatca ccacccggtt tttacttttc gtgctgtgca ttcagcagaa
    20881 atgtgaatcc ctcggctgat ctgaaccatg ataccggcgc aggaacgcat gaaggcagct
    20941 tcgaagcccg gcgacgacac ccgcctcacc cccgacccgt tttccgccgt gctgccggcc
    21001 ttggccgcct tgggagccct cacgtcaatc gcgaccgtga actgggtggc ccaggagcga
    21061 acctccgagc gcaccggggt gagacgcaag gcaaacgccg tgctgcgtga tctcgagagc
    21121 tgctgtgtgg gcctgcagga gatcttcagg agattcgagc gccatccgaa gctgttcgct
    21181 ggcgaaggca aggcggcggc atcgccgctc aagttcggcg tccatggcct gcgcatcact
    21241 cccgatgagg caaggctcta tcaaaaatcg atggacgaca tcgcgtccat gctggtgctc
    21301 gccacgcaaa acagcttcga cgtcatgtcg gccatcgagg acggcgagat cgatgcgccc
    21361 gaggagctgt tctttggttt cggcgagcag caagaacgcc tcaacggatt gctcacgcag
    21421 cgcgccaccc tgaagacgtg cgtcgagacc ggtttgtctg tcgccgaaca actcaccgcg
    21481 cttgttcgcg gcctgcgccg gcatagggtg gaatgacgac aagctccgta tcgacgagcg
    21541 atcggcgtgc agcgaataga cctgttgaca ggcgcccttc aagtctcgca tgtcaggcgc
    21601 cgggtgcgag gcccgatgtc tcgccgagca aaaggactag gggcaatggt ccagcttaga
    21661 ctgccaaaaa actcgacgat cgagcagggg aagacctgga acaggccgag cggaaaaggc
    21721 gactggaaag agatcaaggt ctaccgctgg aacccggacg actcccataa tccgcggatg
    21781 gacacctact ggatcgatcg cgagacgtgc gggccgatgg tgctcgatgc cctcatcaag
    21841 atcaagaacg agatcgacgc caccctgaca ttccgccgtt cctgccgcga aggtgtgtgc
    21901 ggctcgtgtg ccatgaacat cgatggcatc aatacactcg cgtgcaccaa ggctctcgac
    21961 gatgtgaaag gcgcggtctc catttacccg ctgccgcaca tgcgggtggt gaaagacctc
    22021 gtccccgact tgacgaactt ttatgcgcag caccgttcga tcgagccatg gcttaagacg
    22081 tcgagtccga caccgggcga ggaatggaga cagtcccgtg aggatcgggc gaagctcgat
    22141 ggactgtacg agtgcattct gtgcgcttgc tgttcggcag cgtgtccgag ctactggtgg
    22201 aattccgatc gctacctcgg ccccgccatc ttgctgcaag cctaccgctg ggtcatcgat
    22261 tcacgcgatg aggcaaccgg cgagcgcctc gacaatctcg aggatccgtt ccggctctac
    22321 cgttgccaca caatcctcaa ctgcgccaag acgtgcccga agggcctcaa tccggcgaag
    22381 gcgattgcgg agctgaagaa gctgatggtg gagcggcgcg tctgataggc taacgagcgc
    22441 gtcctcgcac gcagacactt agcgccgcga accctccaca aaaaatggac cggagacgct
    22501 ccagcgacct ccggccgatg cgtgcagcac atcatgtgcc gcaggaaaac cagtccgttc
    22561 gtcatcctga tgcccggtca ggacgagaag cagaccgggt caggctgctt tcttcttttt
    22621 cgtagcggtc ttcttggcag tcgctttctt cgcggcaggc tttttcgcag gagcccggcg
    22681 cttcgcaggc gctttcacgg ccgtcttctt ggaaggagcc ttcgcagctg gtgcctttgc
    22741 ggttttcttg gtcggggcct tcgcgcgagc cttgcttgcg ggcctccccg cagttttggc
    22801 cggcgccttg gatgaagttg atggtgtcct tgccataatc ctttctccct cagattgatt
    22861 cgaatcgatt cgaactctgc gagcatgtgc aatcgatttt aatttgttga catcgatcat
    22921 aagttatacg gcgctattca tagtatttct tcggcagtgt tgcgcagaat catcaatttt
    22981 tcgaagcatg aacgggttat cggcggtcga ggagagttcc tgcgagcatg atcggctaac
    23041 tcgatcaatt gccagggcga cccggcggtt gctaaacgct gatgccccgg tctgcgttcg
    23101 gaatggttcc agcctcttcc cgattttccc ggtactgttt catcagctcg cgccattagc
    23161 tgccccgtag cgatgggcat agaacctcgc ggccactgcc gcgtttcact tttcgaagca
    23221 aacgccacct ttggagagat cgatggcaga gctgaagacg aaggtgtcca aggaagaggc
    23281 gctctaccag gatgaaccgc gcgccggcga gaagtgcgag gcgtgtaagc acttcatacc
    23341 ggagcagagc gcatgcgaga tcgtcgccgg ccgtatttca cctcagggat ggtcgaaata
    23401 cttcgagcca gcctgacgaa tgatgtgtgc ggccgcagct gatttctgca gccgcatccg
    23461 ttcaggccgc cttgacgcgc cagttctgaa gatcgagcac ggccatcatc aggcccatgc
    23521 gatcatccat ttcctcggcg aggatcgaga agtagtccgg tccattcggc acgatctcgc
    23581 cgatgacgtc gccgtactcg aagcgaagcg ctgcatcata cttgcgcgca atcgcctgca
    23641 tctttccgtt ttcggcgaga cagctcaggt agaggcgctt gatgccgcgg ttgcgggctg
    23701 cgcggatcac gcggcccatg agctcggtgc caatgccttt gtcttgccat ggccgctcaa
    23761 cggaaaatgc cgcttctgcc tcttcgcccc aaacggcgct gagctttctc aattccgccg
    23821 ctgcgcgaac gtctccccca tcgaggaagc aatagatgat gcttcccata tgatgcaccc
    23881 ggctcgcgta atccacgatg aacgcgtccg agactgcgtg tgcgaagcgc atccgccggc
    23941 tctccgcatc gagccgcagg agatgatccc gaaacttgtc cccctccgtc ggccagagct
    24001 tcctgatcgc ccctccccag atctggtgat cgctcatgat cgcacctcca tctggcagtg
    24061 acccgcgacc gcccggtctt tcgccgggtc tgttgcggtg cacaaatcaa atggatgaat
    24121 gatggcaaaa cgacaagtca ggcttccgct gaccttccct gcagcgattg caaagcatgg
    24181 gcttgcatga gccttcgtct gcttgaccgc tgcatccaag caggctagcc cgagcgccga
    24241 tgttgaaagg acctctggag agttacggtg ctctgatcac tgcgggcggc atcgagccgg
    24301 acgcggcaca gcgccatgct gccgagcgtc tcaatgcact ggcgcaagcg ctcgcccgct
    24361 ggcggccgaa gcgcagtctc ttctcactct tcgccggcgc ttcatctccg cctccgcgcg
    24421 ggctctacat ccacggcgcc gtcggccgcg gcaagaccat gctcatggac ctgttccatg
    24481 ccagcgtcgc attcgagccg aaacgccggg tgcactttca tgatttcatg gccgacgttc
    24541 acgaacggat cgcgcaagcg cgcaagagcg agcccggcga tccgatcccg gccgtcgcgc
    24601 gccggatagc ggacgaggcg aagcttctct gcttcgacga gctgcacgtc accgacatcg
    24661 cggatgcgat gattctcggg cgcctgttca aagtgctatt cgagagcgga gtcgtggtgg
    24721 tcgcaacctc gaattcgccg ccacaggagc tctaccggaa cggcctcaat cggcagctct
    24781 tcctgccgtt cgtggacctc atagaggacc acatggacgt gctggagctg gctgctgcca
    24841 aggatttccg gctcgaaaag ctcaatgggc agcagcttta tttcacccct gccgatgatg
    24901 cagcccgggc tcagctcgat gcgatctggc tatcactgac ggggaacgcc aggggtgaac
    24961 gcaaggacct ggtcatcaag ggccgcacgc tgcgggtgcc gcaggcggcc atgggcgtcg
    25021 cgcgcttcag cttctcggac ctttgcgaaa aaccgctcgg gtcgctcgac tatttgcatc
    25081 tcgcgcgcac ctttcacacg cttctcatcg agcgaatccc catccttgga ccgcagcggc
    25141 gcaacgaggc gcgccgtttc atcaacctca tcgacacgct ctacgacaac cgcgtgtgcc
    25201 tggtggcaag tgccgaagcc gagcccgatc agctctacgt cagcggcgat ggtgccgacc
    25261 tgttcgagcg aacggcatct cgactgatgg agatgcgctc tgaagcctat ctcatggatc
    25321 gcaacagtcg ccgggcgcgc gagcaggtct cgttcggcag ctgagcgccg gctgctggcc
    25381 tgcagctccg ctgctgatct gcggtctacc ttgaacagcc cgcggtttcc ggctaaccac
    25441 ctcgaaacag gatgctgacc gacttggatg cctgactgct tgggggcggc ccgtgttttc
    25501 gagtcggcag gctcaggcga catcgcaggt tcagggagac aagacccatg gcgcgtaaca
    25561 agatcgcgct gatcggcgca ggcatgatcg gcggcacgct ggcccatctc atcggcctca
    25621 aggagctggg ggatgtcgtc ctgttcgaca tcatggaggg cattccgcag ggcaaggctc
    25681 tggacattgc ccaggctggc ggtgtcgagg acttcgattg tgtgctcaag ggcaccaaca
    25741 gctatgccga tatcgttggc gcccaggtct gcattgtcac cgcgggcgta ccgcgcaagc
    25801 cgggcatgag ccgcgacgac ctgctcgaga tcaatctcaa ggtcatgcag caggtcggcg
    25861 agggcatcaa gcagtacgcg cccgatgctt tcgtgatcgt catcaccaac ccgctggacg
    25921 cgatggtctg ggtgatgcag cagaagtctg gcctgcccgc caacaaggtg gtggggatgg
    25981 cgggtgtgct cgacagctcc cgcttccgcc tgttcctggc gcaggaattc ggcgtctcgg
    26041 tcgaggacgt gacggccttc gtgctgggcg gtcatggcga ttcgatggtg ccgctgaccc
    26101 gctattccac agtcgccggc attcctgtgc cagacctcat caagatgggc tggaccaccc
    26161 aggaaaagat cgacgcgatc gtcgcgcgga ccgcgaatgg cggcggcgag atcgtgaaac
    26221 tgcttgagcg t
//
LOCUS       DASVDU010000320        15151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_508837, whole genome shotgun sequence.
ACCESSION   DASVDU010000320 DASVDU010000000
VERSION     DASVDU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508837"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..909
                     /locus_tag="VF226_21355"
     CDS             148..909
                     /locus_tag="VF226_21355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076595.1"
                     /translation="MSVPLGYQLAKRDMQAKYRQTLLGYVWALLPTVATTLAFTFMRS
                     ANLLNTGDTQIPYPLYVLLGAMFWQLFTNALLQPMSIVQSSKSILTKMQFPREALLVT
                     AVLLVLVDFLIKLTLLPVVFLVFGAWPSWDIFLMPVISAAIVVAGMAIGILLVPINLI
                     FQDTKMAVQLILSFMILATPIGYVTPAEGWLRVLVTLNPMSGLIEAARASMTALPMPA
                     LASWGLTTGSSCILLFIALAFYLIALPIVVERQSA"
     gene            1026..2375
                     /locus_tag="VF226_21360"
     CDS             1026..2375
                     /locus_tag="VF226_21360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076596.1"
                     /translation="MFYAVRDLGSEVTGRSVSPVLRPDEFWALQGLSFTLRRGEALGI
                     IGPNGAGKSTLLKIITGIIKPTTGFVRTRGRVQALIELSSGMNPTLTGRENIYIRATL
                     LGMPKSLIKERFDDIVCFAELEDDIDMPVQNYSSGMRVKLGFSIAINVDPDVLILDEV
                     LAVGDQRFRAKAREAMSALLRRDVALIFISHNMGQVLSITDRVLWLDRGKMKEVGPSR
                     EVCSRYLTHQQKAAVDQSGDQPVLINRSIDTFRLEGIQLSHGGQPLPRDFVIPSSETD
                     RIYDLDLEFSAQDDAEEDEFYYGPFVGRNDTDWQAYCYIQDRIRVRSGERFKRRFRID
                     LSRFNPGYYQFGLLILPERDVSVVSFGAYAILRFTVEPREVLQGGWEAGQIHHRRMID
                     NSLGANLLPVEIQGRADSRHPDPSQRKHDMEPRQANNVVHMSASEGSARERQIERKQ"
     gene            2430..3176
                     /locus_tag="VF226_21365"
     CDS             2430..3176
                     /locus_tag="VF226_21365"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7076597.1"
                     /translation="MRERLYDGALALHKQLGYWLGQGFGGRRTLRAEAAAARRFLPSS
                     GAIILDVGAHRGEWSRALLDIAGPQLASIYAFEPAPENHDFIRKIADPRLELVPLAVS
                     DRPEQLVLYSHVPGASISSVYRRKLDHYGIEFDKERSVECTTVDAFLSERDVARVDFL
                     KLDVEGHEMKVLEGAQKGLASGKIRALSFEFGGCNIDSRTYLRDFWFFLNDLGFAIYI
                     INPLSGIHAIEKYHERQESFLTTNYIAVRS"
     gene            3386..4339
                     /locus_tag="VF226_21370"
     CDS             3386..4339
                     /locus_tag="VF226_21370"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7076598.1"
                     /translation="MLASERGAMLMEVLMQRRCLTDLLELNRRKNQWLLNHRRDVTSQ
                     SGEDGVIEKILEFIPDQTEWCVEFGALNGELYSNTYNLIVNRGWSSIQIEGESSYHKR
                     LQQTHAGRENVHCLNQFIGLKKGSTLDEILAKFPIPKDFDLLSIDIDGCDYHVWQSLK
                     NYRPKIVVIEFNPAIPPHITFVQAPDLRLREGSSLRAMWELGKELGYTLVCNTFANAI
                     FVRTDYAKPFGVETDDPEPFFRSRELLFDLFQLFDGTLVVEGNRTLNWSKVPFGSRDL
                     QVMPSFCRGPYWTRFPFIRTKWLQLFHPNRMRNRKRAPKDY"
     gene            4421..5146
                     /locus_tag="VF226_21375"
     CDS             4421..5146
                     /locus_tag="VF226_21375"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX7076599.1"
                     /translation="MPFLNKISAKRKRIKTWMRDIKKARASRRINFFLKRSRGVIHIG
                     ASSGQERDLYAEHDLKVLWLEPIPDVFDQLTENIRSYAEQRALQALLLDQDDVEVALH
                     IASNSGKSSSVLPMKDHKRMWPHVHYDGQLSMRSITLDTLIQREGIDGRSYDTIVMDT
                     QGSELRVLMGARSTLQGITYVKVEAADFESYEGGCTAEDLSAFFGEAGFVEERRQCFS
                     SMPGVGSYYDLVFRRLEKAQQPT"
     gene            5143..5772
                     /locus_tag="VF226_21380"
     CDS             5143..5772
                     /locus_tag="VF226_21380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7076600.1"
                     /translation="MSRRIGAVKTLARVVRNRRLIKRVVAAPTVKLNLGCGSNRQDGY
                     VNVDVRPLPSVDLVAGPKNCVRYFSGRCDEIYISHVLEHFASPGKAKRDHDETVLGFL
                     KTVHMLLKPGGVIRVAVPDFGAIARLYVEGRLPLYPRLLGRLCGEQDYRENLHKCLFD
                     RSFLEQCLTETGFVNVQEWDPKTTGLCRDSSFDELDGVRTSLNLIAEKG"
     gene            5853..6983
                     /locus_tag="VF226_21385"
     CDS             5853..6983
                     /locus_tag="VF226_21385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7076601.1"
                     /translation="MAQEFFYGLPQLRKRGLEVGLLETTELAPDPETWRYRWAARKDR
                     RRSGELGMEHCERLFVEERHRLRAYDLLLAGTEYVALGLADHLRGWRGRARVPMLFFV
                     MGMLSKPLRRFPDGSRQRQRALNRYRNLLQVSRGALFLGEGEYRAALNLFPELHLRLH
                     FVPFGVDTDFWTPGQEAPNASARYVLFIGNDANRDIDMLLQIARAMPEQRFVALSSLL
                     GDRAVPPNLEVIASHWKKAVLTDAEVRAYYRNAAAVLLPIRETLQPSGQSVALQAMAC
                     GCPVVISGFEGFWESGVFSNGEDILFVQPNTPETFIRILSSLTRNPDSARRIGLNGRA
                     RALERYRLDHFADRLAYIVAAAEAESISRKDRPVNENAGSFV"
     gene            7033..8694
                     /locus_tag="VF226_21390"
     CDS             7033..8694
                     /locus_tag="VF226_21390"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7076602.1"
                     /translation="MKKILGVRFGHDAAACLVYEGRIIADVAEERFTRKKNDGSFPVH
                     AIRFCLESAGIKSSDLDAVAVASEYVAPEFAHFFPGLIPLQTTAPVSQDPLPIYMDRF
                     PVAPECRLVPVEHHRAHAASAYYTAGLGRTERALVAVLDGMGDRSSVSIWRGHHNRLE
                     PLASWDVGGSIGRFYSAATEALGWRHGSDEWKLMGLAPYGTARPGLFDRFYPVYGNGE
                     LIEARHYAKVHRFPDHGTFHYHMDEAVALRNLLHDVTREDFAAEAQRVAEEQAQALIL
                     PWLDREDTRTLCCAGGFFLNVKLNGKLWQSGRIDHHWVYPNPGDSGLPAGAALAVYYE
                     AHPEAEMERLEHLYHGPEFSNEEIRAALEARHIPYTRPADLPGTAARLLAENRILGWF
                     QGRMESGPRALGGRSILMSPLRAENKDIINASVKYREAFRPFCPALLDEKKDDYLVQP
                     RSEPFMITGFDAAPGRRDNIPAVVHVDGSLRPQTVRSEVNPRFYALINAFGNLTNEYV
                     VLNTSFNVKGEPIVCTPSDAIKCFFDTGLEYLAIGDFLVAKPGIA"
     gene            8691..10091
                     /locus_tag="VF226_21395"
     CDS             8691..10091
                     /locus_tag="VF226_21395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7076603.1"
                     /translation="MMRAGPSMIETQAVATTVSLERQEAGPLPATVAYIHHGQVPGGA
                     PTSLRILVEEVGRQAPRTKRQIWCVFPPMMPYFAQIDGAQVDKYIETPVLTGKKFIGW
                     SPFFQTKTLGLLLRQLMATRTTIAREVAWLRRNRPDIVHLNSATLWTSAIAARRAGIP
                     LVWHVRETLRGGPWNARKRLYGAFLRRTADAVIAISPQDAASLGSDREGKVNVIYNAV
                     SLDHFMPERHDGRAARKSHGIPEDAFVILSLGGFSYRKGAWQLIRALKILPDRFHLVV
                     AGDHLPRQPVRQTTLMTLRWRLEDLAVRIGWRATQTWRYPERVKAESASVESRLHQVG
                     HLDDVAPLIAACDVLVFAGTIPHFARPIYEAWAMKKPVVAFDTPVMRSEIANGEDGLL
                     VPRDAPEALAAAFTFIAQNATRAREFGERGRIKALGRFDSSANVRAVLNVYRKLMTNL
                     SAKVATATSRDFPKRP"
     gene            10091..11218
                     /locus_tag="VF226_21400"
     CDS             10091..11218
                     /locus_tag="VF226_21400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7076604.1"
                     /translation="MRILLTTEQTQAISTLINLENLRRLNEIDGVTFTAFRQDYKNFD
                     VILFMGYDPRVSEARTVNPAAKIGVVDIRPGTLEAARGADFLVSNGPEMSAMAARYFS
                     NVFEYPIFHEAKKQERAPANEKPVIVTYHGNRAHATSMFPHVTRALEELAQDVPLELH
                     VIYNVQALSPIPEKFLPGRAVQVRTINWHDRVYEQELAQADIGIVPNLTPMHRRREAL
                     QSTAPNDDAFGAHPSEYLVRYKATSNAGRILAFAQHGIPVIADMFPSAAQIIRNGENG
                     YLAHDAFSWYQALRTLCEDPPRRREIGRTLYATYLERYTIAALNHRFVDFCRTLPPAR
                     EAPAALDGADALFDSADPPADPRAGLTAALWRRLRPGIRAS"
     gene            11215..12003
                     /locus_tag="VF226_21405"
     CDS             11215..12003
                     /locus_tag="VF226_21405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7076605.1"
                     /translation="MTRFYDKTENRLVFVARAASSAFWDDHWQSEKLRQLIERGNGFV
                     VRETKRYLPAGARVLEGGCGRANTVWGLHKAGFQAVGVDFAERTVAAVNNAAPELDVQ
                     VEDVRRLSFPDASFDGYWSLGVIEHFYDGFDPILEEMWRVLRPDGYLFMTVPAMSPLR
                     HLKARLGLYARYEETPERIDGFYQFALDPARVIARFEKRGFKLVHRSGMDGIKGLKDE
                     ISFLKGPLQRLYDTPGAGAARVKRLLNRVLAPTYHMAYYVMRRP"
     gene            12084..13193
                     /locus_tag="VF226_21410"
     CDS             12084..13193
                     /locus_tag="VF226_21410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7076606.1"
                     /translation="MSLAAWDANGSFEREVQLYRALRPQVGRLTLVTYGDRRDLDCRK
                     RLDDIKIVCNRFGLANGWYERFLVHGWARTLRRPTIVKTNQMPGARLALDVARTAGAR
                     FVARCGYLHSFFVEQTNKPESAIAKRAREEEQAAFTGADRAVVTTQQIRAMVIERYRL
                     DPAKVEVIPNYVDTMQFSPGPSDGTDERRIIYVGRLDAQKNLLALVTALAGLPGIELA
                     IAGAGSQKEEITRAAAELNVPLTLLGSVSHKALPEVINSGAIFVLPSHYEGHPKALIE
                     AMACGRAVVGTDVPGIRDVIRHEENGLLCPTDPDSVRAAVTRLLNDVPLRDRLGHQAR
                     QDVLTHYALDRIVSRERDLYEELLEAGRGKECSAG"
     gene            13235..13936
                     /locus_tag="VF226_21415"
     CDS             13235..13936
                     /locus_tag="VF226_21415"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7076607.1"
                     /translation="MAGNRLQEVLLGIVQTRVARLPPDEALRFVLGLEAALYPLEGRL
                     SVAYGGGLHTKHRHMRYHDFFVDRIASGERVLDIGCGLGKLAYEIAERSGAIVTAVDL
                     VPRNIETATQRFAHPQVTYKVADATQEMEEGPFNVVVLSNVLEHLHERPDFLRRVRAV
                     ALPDRFLIRVPLFERDWRVPLKKELGLEWRLDQDHKVEYTYEAFVGELREAGLRLTYH
                     EIRWGEIWAEAVPNG"
     gene            13929..14975
                     /locus_tag="VF226_21420"
     CDS             13929..14975
                     /locus_tag="VF226_21420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7076608.1"
                     /translation="MAEPQISPQVSVLMSAYNAEAHLHDAVESILGQTFTDFEFIIVD
                     DGSTDATPAILASCGDPRIRILTNDRNVGLTASLNRGLAVCRGAYVARQDADDRSLPQ
                     RLEKQVRWLGEHPEVALVGTAYRAFDEARGIDAIHRLPATDGAIRWHMLFHNAFCHSS
                     VMFRAEATGRHGSSYDETLPFAQDYEFWQRLLQSGRGANLPDPLVEHRVHGDAITSTR
                     FAEQQAVADEIARRQLARLLGEDRVGTLDIRRLRLWFQRLPDDLSWRDLRDVAIFFEL
                     LDRLAATECIGDDELATLRRALQVRFARVLGFPMVILAQKTGFLCGDGALRNWLRAHL
                     PLRLRSAVRADVPR"
BASE COUNT         2964 a         4642 c         4532 g         3013 t
ORIGIN      
        1 ggcgcctgat cccgcatgtt gaaaaccgcg gcaccgtttc ggcgaaccca tcagcagctt
       61 cagcctatgg aagaaagaat caactccgcg gaaagcgtcc tgcgccaccc cggccccgcc
      121 ttgcgaagct ggtggagcga agcgcgtatg tcggtccctc tcggatatca actggcaaag
      181 cgggacatgc aagccaagta ccgccagacc cttctcggct atgtctgggc cctgctgccc
      241 actgttgcga cgacgctggc attcacgttc atgcgcagtg caaacctcct caataccggc
      301 gatacgcaga tcccctatcc gttgtacgtg cttctcggcg cgatgttctg gcaattgttt
      361 acgaacgcgc tcttacagcc gatgagcatc gttcagtctt cgaagtcgat cctcaccaag
      421 atgcagttcc cgcgtgaggc tctcctggtt accgccgtcc ttctggtttt ggtcgacttt
      481 ctgatcaagc taactctgct gccagtggta ttcctagtgt tcggcgcctg gccctcctgg
      541 gacatctttc tgatgcccgt catatcggcg gccattgtcg tggctggcat ggcgatcggc
      601 attctgctgg tgccgatcaa cctgattttc caggacacca agatggccgt gcagttgatc
      661 ctgtcgttca tgattctggc gacacccatc ggttacgtca cacctgctga gggatggttg
      721 cgcgtcctgg tgaccctcaa cccaatgtcg gggctgatcg aggcagcccg ggcaagtatg
      781 acggcattgc caatgccggc cctcgcaagc tgggggctga cgaccggcag ttcttgcatt
      841 cttttgttca tcgcgttggc cttttatctt attgcgcttc cgatcgtcgt cgagaggcag
      901 agcgcatgac gtccgagctt atcggcgtcg aggcaccgcc ggccgccgac tccggaaccg
      961 acggcgagac gctggtcgag gtcgaggggg tttcgaagaa gttcgcgcgc agcctacgca
     1021 agacgttgtt ctatgccgtc cgggatctcg gcagcgaggt gaccggtcgg tcggtaagtc
     1081 ccgttcttcg accagacgaa ttctgggcgc tgcaagggct gtcattcact ttgcgtcgcg
     1141 gcgaagcgct cggcatcatc ggccccaacg gcgccgggaa gtcgaccctg ctcaagataa
     1201 tcaccggcat cataaagccg accaccgggt tcgtccggac ccgcggacgt gttcaggcct
     1261 tgatcgaact cagcagcggc atgaatccca cgctcacagg gcgtgagaac atctatatcc
     1321 gcgccacgct gcttggcatg ccgaagtcgt tgatcaaaga acgcttcgac gacattgtgt
     1381 gctttgccga gttggaggac gacatcgaca tgccggtgca gaattacagc agcggcatgc
     1441 gcgtcaagct gggtttctcg atcgccatca atgtcgatcc ggatgttctg atcctcgacg
     1501 aggttcttgc ggtcggcgac cagcgcttcc gcgccaaggc gcgcgaggcc atgtcggcgc
     1561 tgctgcggcg tgatgtcgcc cttatcttca tttcccacaa catgggtcag gtcctgagca
     1621 tcaccgaccg cgtgctgtgg cttgaccgtg gcaagatgaa ggaggttggt ccgtcgaggg
     1681 aggtgtgctc ccgatacctg acgcatcagc agaaagcggc tgtggatcag tcgggcgatc
     1741 agccggttct gatcaacagg agtatcgata ccttccgcct cgaaggcatt cagctgtctc
     1801 acggaggcca gccgcttccg agggacttcg taattccgtc atccgagaca gaccggatct
     1861 atgatctcga tctggagttc tccgcccaag atgatgccga ggaagatgaa ttctattacg
     1921 ggccttttgt cgggcggaac gacacggatt ggcaggctta ctgttatatc caggacagaa
     1981 tacgcgtcag gtctggtgag cgcttcaagc gccgattcag aatcgatctt tcccggttca
     2041 atcccggcta ttaccaattt gggcttctga ttctgccgga acgtgacgtt tcggtcgtat
     2101 cattcggagc ctatgcaatt cttcgtttca ccgtggagcc cagagaggtt cttcagggtg
     2161 gctgggaagc tgggcaaatc caccatcggc gaatgatcga caactccttg ggtgcgaatc
     2221 tcctgcccgt ggagattcag ggtcgtgccg actcgcggca ccccgatcca agtcaacgga
     2281 agcatgatat ggaaccacgt caagcaaaca atgtcgtgca catgtctgcc tcggaaggca
     2341 gcgctcgcga gcgccaaatt gaacgcaaac aatgacaggc aagcaccttc ctaccgcagg
     2401 gcgccatgaa cgaatgagag atatggtcaa tgcgtgagcg cctttacgac ggagctctag
     2461 cgctccacaa gcagctcggt tattggcttg gacaaggctt cggagggcgc cgcacgctcc
     2521 gcgcggaagc tgccgccgca cgacgctttc taccctcttc cggcgctatc atacttgatg
     2581 tcggcgcaca ccgcggtgaa tggagccggg cgctcctcga catagccggc ccgcaactgg
     2641 caagcatcta tgcctttgag ccagcacccg agaatcacga tttcatcagg aaaattgccg
     2701 acccgcgcct cgaactcgtt ccgctcgccg tcagcgaccg cccggaacag ctcgttcttt
     2761 acagccacgt tccaggcgcc agcatatcct cggtctatcg ccggaaactg gatcattacg
     2821 gcatagagtt cgataaagaa cgctccgttg aatgcaccac cgtcgatgcg tttctctcgg
     2881 agagggatgt cgcaagggta gactttctca agctggacgt cgaagggcac gagatgaagg
     2941 tgctggaggg agctcagaag ggcctagctt cagggaaaat tagagcctta tcgtttgaat
     3001 tcggcggatg caatatcgat tccagaactt acttgcgcga tttctggttc ttcttgaatg
     3061 atctcggatt tgcaatttac atcatcaatc cgctgtcggg catacatgct atcgagaaat
     3121 atcatgaacg gcaggaatct ttccttacga caaactatat tgccgttcga agctagacag
     3181 gcttcgagtc gcgactgggc gagttgagaa ggtaataacg gtgcagcgtt caacggtgac
     3241 gacgagacaa gacccggcgc ctgatgacgt cggccacgag cgagcctcgg agatcataca
     3301 gacggaagag tccgattttc ggaatatcgt cctgaaccac gcccgaaagg ccctgaatcg
     3361 ggaggacatt ggagcgcttc aggacttgct ggcgtcggaa cgaggagcga tgctcatgga
     3421 agttctgatg cagcgccgtt gcctgacgga cttgctcgag ttgaacaggc ggaagaatca
     3481 atggctcctg aatcaccgtc gggatgttac ctcgcaatca ggcgaagacg gtgttatcga
     3541 gaagattctt gagttcatcc ccgatcaaac ggaatggtgc gtcgagtttg gcgcactgaa
     3601 tggagaactc tacagcaata cgtacaattt gatcgtgaac cgtggctggt catccatcca
     3661 gatcgagggt gagtcatcct atcacaaaag gctccagcag actcatgcgg gcagggagaa
     3721 cgtccattgc cttaaccagt ttatcgggtt gaaaaaagga agtaccctcg acgaaattct
     3781 tgcgaaattc ccaattccga aggacttcga cctcttgtcg atcgatattg acggatgtga
     3841 ttatcacgtc tggcagtcat tgaagaacta caggccgaag atcgtcgtta tcgagttcaa
     3901 cccggccatc cccccccaca tcaccttcgt ccaggcgccg gacctccggt tgcgcgaagg
     3961 cagctccctc cgcgcaatgt gggagctcgg caaggagttg ggctatacac tggtctgcaa
     4021 caccttcgcg aatgcgatat tcgtccgaac cgattacgcg aagcccttcg gtgtggaaac
     4081 agatgatccc gaaccgttct ttcgatcccg ggagcttctc ttcgatcttt tccagctctt
     4141 cgatggcacg ctcgttgtcg agggcaaccg gaccctcaac tggagcaagg tgcccttcgg
     4201 cagccgagat ctgcaggtga tgccgtcttt ctgtagaggg ccgtactgga cgcgctttcc
     4261 cttcatcagg acgaagtggt tgcagctgtt ccacccgaac aggatgcgga atcgcaaacg
     4321 tgctcccaag gattattgac agcgttttta ccggctgctc tgccatagac agaacggtcg
     4381 agaatgtccg ttaggcgccg aataatttag agacaggtcc gtgccgttcc tcaacaagat
     4441 ttctgcaaaa cgaaaaagaa taaaaacgtg gatgcgagac attaagaagg cgagagcatc
     4501 ccgacggatc aatttttttc tcaagcgctc gcgcggcgtg atccacatcg gcgcaagcag
     4561 cggccaggaa cgcgacctct acgcagagca cgatctgaaa gtcttgtggc ttgagcctat
     4621 cccggacgtt ttcgaccaac tgaccgagaa cataaggagc tatgcggaac aacgagcgct
     4681 tcaagccctg ctcctcgatc aggacgatgt cgaagtcgcg ttacatatcg cgagcaactc
     4741 gggaaagtcg tcatccgtcc tgccgatgaa agatcacaag cggatgtggc ctcatgtgca
     4801 ttatgacggg cagctttcca tgcgtagcat cactctcgat actctgatcc aaagagaggg
     4861 catcgacggg cggagctatg acacgatcgt catggacacg cagggatcgg agctccgcgt
     4921 ccttatgggc gcccgttcta ccttgcaggg gatcacttac gtcaaggtag aggcagcgga
     4981 tttcgaatcc tacgaaggcg gatgtacggc cgaggacctg agcgccttct tcggcgaggc
     5041 aggcttcgtc gaagaacggc gccaatgctt ctcgagcatg ccgggcgtcg gcagctatta
     5101 cgacctggtc tttcgtcggc tggaaaaagc gcagcagccg acatgagtag acgaatagga
     5161 gcggtgaaga cgctggcgcg ggtggttcgg aaccggcgcc tgatcaagcg ggtggttgcg
     5221 gccccgaccg tcaaactcaa cctcggctgc ggcagcaacc ggcaggatgg atatgtcaat
     5281 gtcgacgtcc gaccgctgcc gagcgtcgac cttgtcgcag ggccgaagaa ttgcgttcgg
     5341 tatttttccg gccgctgtga cgagatctat atctcccatg tccttgagca tttcgccagc
     5401 ccgggcaagg cgaagcgcga tcacgacgag acggtcctcg gcttcttgaa gactgtccac
     5461 atgcttctca aaccgggcgg tgtcatacgg gtcgcggttc ctgacttcgg ggcgattgca
     5521 aggctttatg tggaaggccg cctcccgctc tacccgcgcc tcctcgggcg actgtgcggc
     5581 gagcaagact accgggaaaa cctccacaaa tgcctgtttg accgctcctt tctcgagcag
     5641 tgcctgaccg aaacaggatt cgtgaacgtg caggaatggg atccgaagac caccggtctg
     5701 tgccgcgatt ccagtttcga cgagctcgac ggcgtacgaa ccagcctcaa cctgatagcc
     5761 gagaaaggtt gatggagagt cggccgcgtg tcctgttcgt ccatgccggt gggcgtaggg
     5821 aacggcttgc gcagctagag cagggcgttc caatggcgca ggagtttttc tacggcctgc
     5881 cccaactgcg aaagcgggga ttggaggtcg gcctcctcga aacgacggaa ctcgcacccg
     5941 atccggaaac ctggcgctat cggtgggccg cccgcaagga ccgtcggcgg agcggtgaat
     6001 tgggaatgga gcactgcgag cgcctattcg tcgaagagag gcatcgccta cgggcctacg
     6061 atctcctact cgccggtacc gaatatgtcg ccctcggcct tgccgatcat ttgcgaggct
     6121 ggcgtgggcg ggcgcgcgtg cccatgctgt tcttcgtcat gggtatgctc agcaagccgt
     6181 tacgccgctt ccccgacggc agccgacaac ggcagcgggc actgaacagg tatcggaatc
     6241 tgttgcaggt gtcgcggggc gctctgtttc tcggcgaggg tgagtatcgc gcagcgctga
     6301 atctcttccc cgagctgcat ctgcggttgc acttcgtccc cttcggggtc gatacggatt
     6361 tctggacacc gggtcaggag gcgccgaacg caagcgcgcg atatgtcctc ttcatcggca
     6421 atgacgcgaa ccgggacatc gacatgctgc ttcagatcgc gcgggcgatg ccggagcagc
     6481 ggttcgtggc gctgtcgtca ctgctcgggg acagggccgt tccgcccaac ctggaggtca
     6541 tagccagtca ttggaagaag gccgttctga cagacgcgga ggttcgcgcc tactaccgga
     6601 acgccgcggc cgttctcctt cccatcaggg aaacccttca gccgtcggga cagagcgtcg
     6661 cgcttcaggc gatggcgtgc ggctgcccgg tcgtgatctc ggggttcgaa ggcttttggg
     6721 aaagcggcgt tttcagcaat ggcgaggata ttctcttcgt ccagccgaac acgccggaga
     6781 cgttcatcag aattctgtcg agcttgaccc gcaatccgga ctcggcccgc cggattggat
     6841 tgaacggtcg ggcgcgagcg ctcgaacggt atcggcttga ccatttcgcc gaccgactcg
     6901 catacatagt ggcagcagcg gaagccgaat ctatctctcg aaaagaccgc cccgttaacg
     6961 aaaatgcggg ctcgttcgtc taagcaatac gcggcgatgt gttgtagaaa atagaaaaat
     7021 gaagccttgg cgatgaagaa aattcttggt gtccggtttg gccacgacgc agcggcatgt
     7081 ctggtctatg aggggcgcat cattgcagat gtcgcggaag agcgctttac ccgcaagaag
     7141 aacgacggct cctttccggt ccatgccatt cgcttctgcc tggagtccgc cgggatcaag
     7201 tcgtccgatc tcgatgccgt ggccgttgcc tcagagtatg tggcgccgga gttcgcccat
     7261 ttcttcccgg gcctgattcc actgcagacc actgcgcccg tgtcacagga cccactcccg
     7321 atctacatgg atcgttttcc agtcgccccc gaatgtcggc tggtgcccgt cgagcaccac
     7381 cgcgcccacg ccgcgagcgc ctattacacg gccggcctcg gccgaacgga gcgagcattg
     7441 gtcgccgtgt tggacggcat gggcgaccgc tcttctgtga gcatttggcg agggcaccac
     7501 aatcggctcg agccgctggc ctcttgggac gttggaggat ccatcggccg attctactcg
     7561 gccgccaccg aagccctcgg atggcgccac ggaagcgacg agtggaaact gatggggctc
     7621 gcaccttacg gcacggcccg gccaggcttg ttcgatcgct tctatcctgt ctacgggaac
     7681 ggtgagctga tcgaggcgcg gcactacgcc aaagtccatc gcttccccga tcacggcact
     7741 tttcactatc acatggatga ggcggtggcg ctccggaacc tgttgcacga cgtgacccgg
     7801 gaggatttcg ccgcagaggc acagcgcgtc gcggaagagc aggcccaggc ccttattctg
     7861 ccttggctgg accgtgaaga cacgcgcacg ctctgctgcg ccggcggctt cttcctgaac
     7921 gtgaagctca acggaaagct ttggcagtcg ggacggatcg atcatcactg ggtctatccc
     7981 aacccgggtg actcaggcct gccggcagga gccgccctgg ctgtgtacta cgaagcgcat
     8041 cccgaagccg agatggagcg cttggagcat ctctaccacg gccccgagtt ctccaacgag
     8101 gagatccggg cagcattgga ggcccgccat attccataca cccgcccggc ggatcttccc
     8161 ggaacagcgg cccgactcct ggctgaaaac agaattctcg gctggttcca gggccgcatg
     8221 gagtccgggc cccgggccct cggaggccga tcgatcctga tgagtccgct acgcgccgag
     8281 aacaaggaca tcatcaacgc atcggtgaag taccgcgagg cctttcggcc gttctgcccg
     8341 gccctgttgg atgagaagaa ggatgactat ctcgtgcaac cgcgctccga gcccttcatg
     8401 atcaccggtt tcgatgcggc tcccggtcgg cgcgacaata ttccggcggt ggtccatgtc
     8461 gacggatcgt tgcggccgca gaccgtacgg agcgaggtaa acccgcgctt ttatgccctg
     8521 atcaacgcgt tcggcaacct cacgaacgag tatgtggtcc tcaacacatc cttcaatgtg
     8581 aagggtgaac cgatcgtctg tacgccgagc gacgcgatca agtgcttctt cgataccggt
     8641 ctggaatatc tggcgatcgg cgattttctc gtggcgaaac ccggcatcgc atgatgcgcg
     8701 ccggtccatc gatgatcgag actcaagccg tcgcgacgac ggtgtccctt gaacggcaag
     8761 aagccggccc gctcccggca acggtagctt acatccacca cgggcaggtt ccgggcggcg
     8821 cgccgacgag cttgcgcatt ctggtcgagg aagtcggacg ccaggcgccg cgaaccaagc
     8881 ggcagatctg gtgtgtcttt ccgcccatga tgccttactt cgcgcagatc gacggagcgc
     8941 aagtcgacaa atacatcgaa accccagtcc tgaccggcaa gaagttcatc ggctggagcc
     9001 cgtttttcca aaccaagacg cttggtctgc tactgcgcca gctgatggcc acccggacaa
     9061 cgattgcccg cgaggtggcc tggttgcgtc gcaatcgacc ggacatcgtg catttgaact
     9121 ccgctactct gtggacatcg gccatcgcgg cacggcgcgc cggcatcccg ctcgtttggc
     9181 atgttcgcga gaccttgcgt ggcggacctt ggaatgcccg caagcgcctt tatggggcct
     9241 tcctgcgtcg taccgccgat gccgtcatcg ccatcagccc acaggatgcg gcgagcctcg
     9301 gcagcgaccg cgagggtaag gtcaacgtca tatacaacgc ggtcagtttg gatcacttca
     9361 tgccggaacg ccacgatggg cgggctgcgc gcaagtccca cggcatcccc gaggatgcct
     9421 tcgtgatcct gagcctcggc ggcttctcct atcgcaaggg cgcgtggcag ctcatcaggg
     9481 cgctgaagat actgcccgac cgcttccacc tcgtggttgc gggcgaccac ctgccgagac
     9541 agccagtacg ccagacgacg ctcatgacgc tccgatggcg gcttgaggac ttggcggttc
     9601 ggatcggctg gcgggcgacg cagacctggc gctatcccga gcgcgtgaaa gccgaatccg
     9661 ccagtgtcga aagccggctc catcaagttg gtcatctcga cgatgttgca cctctaatcg
     9721 cggcctgcga cgttctcgtc ttcgctggaa ccatccccca tttcgcccgc cccatctatg
     9781 aggcctgggc catgaagaag ccggtcgtgg cattcgacac gccggtcatg cgatccgaga
     9841 ttgcgaacgg cgaggatggt cttctcgttc cgcgtgacgc gccggaagcg ctggccgcag
     9901 ctttcacgtt catcgcacag aacgccaccc gcgcccgcga gttcggagag cggggtcgca
     9961 tcaaggctct cggccgtttc gacagcagtg cgaacgttag ggccgtgctg aacgtctatc
    10021 gtaagctcat gaccaacctt tccgccaagg ttgccactgc gacctcgcgc gactttccga
    10081 agaggccgtg atgcgtattc tgctcacgac cgagcaaaca caggccattt caacacttat
    10141 caatctggag aacctccgcc gcctcaacga gatcgacggc gtcaccttca ccgccttccg
    10201 ccaggattat aagaacttcg acgtaatctt gttcatgggc tacgacccgc gggtatcgga
    10261 ggctcgtacc gtcaacccgg cggcaaagat cggcgtggtc gatattcgcc cggggacgct
    10321 cgaagcggcc cgcggtgccg actttctcgt ctccaatggc cccgagatga gtgccatggc
    10381 cgcgcgttac ttctcgaacg tcttcgagta cccgattttc cacgaggcca aaaagcagga
    10441 acgggcgcct gcgaatgaga aaccggtgat cgtcacttat cacggcaacc gcgcgcacgc
    10501 gacatcgatg ttccctcatg tgacccgggc gctcgaagag ctggcgcagg acgtgccgct
    10561 ggagcttcac gtcatctaca atgtccaggc cctatccccg atacccgaga aattcctccc
    10621 cgggcgagcc gtccaggtgc gcaccatcaa ttggcatgat cgcgtatacg aacaagagct
    10681 tgcacaagcc gatatcggta tcgtgccgaa cctcacgccg atgcaccgtc gacgcgaggc
    10741 actgcagagc acggcaccaa acgatgacgc cttcggcgcg catccgagcg agtacctggt
    10801 ccgctacaag gcaaccagca atgcgggccg tatcctggct ttcgcgcagc atggcatacc
    10861 cgtgatcgcc gatatgtttc catcagcagc acagatcatc cgaaacggtg aaaacggcta
    10921 tctcgcccat gatgcgttca gttggtatca ggctcttcga accctctgcg aagatccacc
    10981 gaggcgtcgg gaaataggcc ggacgttgta cgccacctac ctggagcgct acacgatcgc
    11041 agccttgaac catcgattcg tcgacttctg ccgcaccttg ccgccagcac gtgaagcccc
    11101 cgccgcactt gatggcgccg acgcgctctt cgacagtgcc gacccgccag cggatccgcg
    11161 cgcaggcttg acggcagctt tatggcgtcg actccgtcca ggtatccgag cctcatgaca
    11221 aggttttacg acaagaccga gaatcgcctg gtcttcgtcg cgcgggccgc ctcctccgcc
    11281 ttctgggacg accattggca gagcgagaaa ctgcgccagc tcatcgagcg ggggaacggt
    11341 ttcgtcgtgc gcgagacgaa gcgatatctt cccgccgggg cgcgggtgct ggagggcggc
    11401 tgcgggcggg ccaatacggt ctggggactg cacaaggccg gctttcaggc cgtcggcgtc
    11461 gattttgccg agcggacggt ggcagcggtc aacaatgctg cgccggaact cgatgtccag
    11521 gttgaggacg tgcggcgcct ctccttcccg gacgcatcct tcgacggata ttggtcgctc
    11581 ggcgtcatcg agcatttcta tgacggcttc gaccccattc tcgaggagat gtggcgcgtg
    11641 ctgcggccgg acggctatct gttcatgacg gtcccggcca tgtcgccgct gcggcatctg
    11701 aaagcgcggc tcggcctgta tgcgcgctac gaggagacgc cggagcggat cgacggcttc
    11761 taccagttcg cgctcgaccc ggcgcgcgtc atcgcccgct tcgagaagcg gggcttcaag
    11821 ctcgtccaca ggagcggaat ggacggcatc aagggcctca aggacgagat ttcgttcctt
    11881 aagggaccgc tgcagcgtct ctacgatacg ccgggggcag gggcggcacg ggtgaagcgg
    11941 ctcctgaacc gcgtcttggc gccgacctat cacatggcgt actacgtcat gcgccgtccc
    12001 tgatccggct ccgcctactc aacctgcaca gctccatgtc ctcgctccgc tccgcccacc
    12061 tcgtcctgtt catgacccgc ggaatgtccc tggccgcgtg ggacgccaac ggctccttcg
    12121 agcgggaggt gcaactctac cgtgcgctgc gaccgcaggt cgggcgattg acgctggtca
    12181 cctacggcga tcgtcgcgac ctcgactgcc gcaagcgcct ggacgacatc aagatcgtct
    12241 gcaaccgctt cggcctggca aacggttggt acgagcgctt tctggtgcat ggctgggccc
    12301 gtaccttgcg ccggccgacg atcgtcaaaa cgaaccagat gcccggcgcc cgcttggcgc
    12361 tggacgtggc ccgcaccgcc ggcgcccgtt tcgtggcgag atgcggttac ctgcattcct
    12421 ttttcgtcga gcagacgaac aagccggaat cggccatcgc aaaacgagcg cgtgaagaag
    12481 aacaggcggc ctttaccggc gccgaccggg cagtggtaac gacccagcag atccgcgcga
    12541 tggtgatcga gcgctaccgg ctcgatccgg ccaaagtcga agtcattccc aattatgtcg
    12601 acaccatgca gttctcaccc ggccccagcg atggaaccga cgaacggcgg atcatctatg
    12661 tggggcggct tgacgcacag aagaacctcc tcgcgctcgt taccgctttg gccgggcttc
    12721 ctggcatcga actggcaatt gccggtgctg gcagtcagaa ggaggagatc acccgggcag
    12781 cggccgagct gaatgtgccg ttaacccttt tagggtcggt atcccacaag gccctcccgg
    12841 aggtgatcaa cagcggcgcg attttcgtct tgccatcaca ttacgaaggc catcccaagg
    12901 cgctgatcga ggccatggca tgcggacggg cggtcgtcgg caccgacgtg cccggcattc
    12961 gcgacgtgat ccgccacgag gagaacggtc tgctgtgtcc cactgatccc gacagcgtgc
    13021 gggctgccgt gacgcgtctg ctgaatgacg tcccgctacg agaccggctc ggtcatcagg
    13081 cacggcagga tgtgctgact cattacgcgc tcgaccgcat cgtgagtcgc gaaagagatc
    13141 tctatgagga attgctcgag gcaggaagag gcaaagaatg ttcggccggt taacgcgacg
    13201 ggttttgacc gcgttgccgc gctggatcga gcggatggcg ggcaatcgcc tgcaagaggt
    13261 cctgcttggc atcgtgcaga cccgcgtggc acgcctgccg cccgacgagg cgctccgctt
    13321 cgtcctgggg ctggaggcgg cgctctaccc gcttgaaggg cggttatccg tggcttatgg
    13381 cggtgggctt cacacgaaac accgccacat gcgctatcac gatttcttcg tcgaccgtat
    13441 cgcctccggc gagcgcgtgc tcgatattgg ctgcggtctc ggcaagctcg cctacgagat
    13501 cgccgagcgg tccggcgcaa tcgtcacggc cgtcgacctc gttccccgca acatcgaaac
    13561 tgcgacgcag cgcttcgccc accctcaagt gacttacaag gtcgccgatg cgacccagga
    13621 aatggaggaa ggtcccttca acgtggtggt gctctccaat gtgctggagc atctgcatga
    13681 gcggcccgac ttcctgcgcc gcgtccgcgc ggtcgccctc cccgaccgct tcctgatcag
    13741 ggtgccgctg ttcgagcggg actggcgggt tccgttgaag aaagagctgg gcttggagtg
    13801 gcgactcgac caggaccaca aggtcgagta cacatatgag gcgttcgtgg gggaactgcg
    13861 ggaggccggt ttgcggctaa cctatcacga gatccgatgg ggcgagatct gggccgaggc
    13921 tgtgccgaat ggctgagccc cagatttcac cccaggtctc cgtgctgatg agcgcctaca
    13981 atgcggaagc gcatctgcat gatgccgtgg agagcattct cggacagacc ttcaccgact
    14041 tcgaattcat catcgtcgat gacggctcga cggatgcgac accggccatc cttgcgtcct
    14101 gcggtgaccc gcgcattcgt atcctgacca acgaccgcaa tgttgggctt accgcctcgc
    14161 tcaaccgggg cctcgccgtt tgccggggcg cgtacgtcgc ccgccaggat gccgatgacc
    14221 gctctctgcc acagcgcctc gagaaacaag tccgatggct tggtgaacat ccggaggtgg
    14281 cgctcgtcgg aaccgcctac cgcgccttcg atgaggctcg cggcatcgac gcaatccacc
    14341 gcctgcctgc gaccgatggc gccatccgat ggcacatgct gtttcacaat gccttctgcc
    14401 atagctcggt gatgttccgg gccgaggcaa cggggcgaca cggatcgagc tacgacgaaa
    14461 ccctgccgtt cgcgcaggac tacgagttct ggcagaggct tcttcagtca ggccgcggcg
    14521 ccaacctgcc cgacccgctg gtggagcatc gcgtccacgg tgacgcgatc acctcgaccc
    14581 gcttcgccga gcagcaggcc gtggcggacg agatcgcccg gcggcagctc gcgcgcctgc
    14641 tcggcgaaga ccgcgtggga acattggata tccgccggct gcgactgtgg ttccagcggc
    14701 tgccggacga tctctcctgg cgagaccttc gtgatgtcgc gatattcttc gagctgctgg
    14761 accgcctggc agccaccgaa tgcatcggcg acgatgagct cgcgactctg cgtcgggcgc
    14821 tacaagtgcg tttcgcccgc gtcctcggat ttccaatggt catcctggca cagaaaaccg
    14881 ggtttctatg cggcgatggc gccttgcgga actggctccg cgcccacctt ccactcaggc
    14941 tccgcagcgc cgttcgtgca gacgtgccgc gctaacgccc cccactcgac ctcagcagat
    15001 gcttacactc ttcagctctc cgaaaccgtt catcggtcat gccggcatca tccagcgcaa
    15061 tgctatgctc agctggacga ggctgtatcc tcgcgccgag gttatcctct tcggcaatga
    15121 cccgggcacg gccgaggtgt gtgagcgatt a
//
LOCUS       DASVDU010000321        44625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_121288, whole genome shotgun sequence.
ACCESSION   DASVDU010000321 DASVDU010000000
VERSION     DASVDU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 44625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44625
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121288"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF226_21425"
     CDS             complement(<1..218)
                     /locus_tag="VF226_21425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076609.1"
                     /translation="MDDNSANGPANKMWGGRFAMSPAEIMEEINASIGFDKALAPQDI
                     RGSKAHAAMLAAQGIISKEDEASIQAGL"
     gene            473..928
                     /locus_tag="VF226_21430"
     CDS             473..928
                     /locus_tag="VF226_21430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7076610.1"
                     /translation="MAAFVFKKAPEPLPEFTFMDGSGAERTLKDWQGRVVLLNLWATW
                     CAPCRKEMPTLERLEQALGSDKFQVLALSVDRGGAEASKKFFDQISVEALDLFVDPTG
                     RATSQLKAVGLPTTLLIDAQGREIGRLTGPAEWDSEDAKRLIQAVLGSS"
     gene            1275..2297
                     /gene="dctP"
                     /locus_tag="VF226_21435"
     CDS             1275..2297
                     /gene="dctP"
                     /locus_tag="VF226_21435"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7076611.1"
                     /translation="MRLVRKLARFVASGVILAAAGVAANAAEFNWRIQSNLNPGEPGY
                     VAVEEKFAKLAEEMSGGRIAFQVFPVGALFPVQEGLEAVGSGIVEMGILTGGYFAGKI
                     GPIANLENGVPGSLRTPIERYNFFYKYGFLDLVREAYGKHGIFYLGPQLSPPWDIVSK
                     KPITSAEDFKGLKIRSFGLEAKWYEKMGATPVFMGGGDIYTGLATGAIDAARWASPSG
                     NKNNSYHEVAKYYVQPSPMPVPNNFFAVNMAAWNQLPNDIKAILNEAAVASSFDYIAR
                     SAMTDAKAMRDMQAAGVQISVIPEDEWAKMESAARELWEAYADQDEFAAKGVDLLKKF
                     LAELGR"
     gene            2429..2935
                     /locus_tag="VF226_21440"
     CDS             2429..2935
                     /locus_tag="VF226_21440"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEX7076612.1"
                     /translation="MLERLIGWIDTAVAAIGKGASFLTLLMTALIVLEMVSRSFFGTS
                     WGWVYDLSGWLLAAYIFLGGAWTLQRGQFVRVDILFTRFSPRTRALVDLVAGTALFVL
                     FAGALLWLGTRFAWQSFLMNEVSATGAWDAPVYVAKALLPIGVVLLCLAWLSHILKLW
                     RNGLRSPE"
     gene            2977..4242
                     /locus_tag="VF226_21445"
     CDS             2977..4242
                     /locus_tag="VF226_21445"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX7076613.1"
                     /translation="MFLCLAIGVPIAIALGGLTVAMIYGFWSPNALTMLPIKAFSSAS
                     SFEFLGIPLFIFMAAMLQRSKIADDLYDSMYRFFGGVRGGLAIGTVAICTLFAAMAGI
                     SGAATISMGLLAIPAMLARNYQKGIALGCVAAGGSLGILIPPSVTMIIYGLIANTSVG
                     KLYAGGVLPGLLIALLFCVYIYVRGVIQPEVVGSTLERFTWREKLAAVKGLVLPILLV
                     VTVLGSMLGGYASVSEAAAVGSFGSLIAAAVRRQLNWTTVREACEETILLSCLIFWII
                     IGASALSTFYTAMGATRLIESMILGLEVNRYIILAGMQLILLLLGMVLDTVGIIMITV
                     PVFVPIALKLGFDPVWFGILFIINMEIGFLTPPFGYNLFYLKGVVPPSITLGDIYRSV
                     TPFILLMIAAVGICMLFPQIVLVLPNALF"
     gene            complement(4251..5042)
                     /locus_tag="VF226_21450"
     CDS             complement(4251..5042)
                     /locus_tag="VF226_21450"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7076614.1"
                     /translation="MDHPNEVWRDDDRKGPFERYLSILEIVAAAHDGLTMTDIAKIID
                     LPKPTVHRLTRVLLDSGALVAEDHRQRRFRIGPRLWRILYLGFDRDALTAYAQMVCDS
                     AAGRLKETCYVVRLGRTDVRTVARATPDQGYRLHVYPGSDLPAHAAASAKAILAHQPQ
                     KAVTRYIREPLARLTPFTKTDISAYLAELEETRTRGYAICDREIDENVMAYAVPVRLP
                     DAGVLFSVGVTGPCSRLLQNPVEFYISGLKEAADQFARALQALEK"
     gene            5194..7227
                     /locus_tag="VF226_21455"
     CDS             5194..7227
                     /locus_tag="VF226_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX7076615.1"
                     /translation="MSTISIGVDVGGTFTDFLMIDTARREYRVAKVPTTVDDRARGFL
                     QGLTELDADPKAIGWLVHGTTVGTNAILERKGARCGLITTRGFRDALELGRRTRPYAY
                     GLSGTFEPLIPREARLEVRERLDAAGRVIEPLNEDDVRAAVQKLLEMDIESVVIHFLH
                     SYANDAHEKRCAQIVRSLWPNANVIAGHEIIREMREFERGSTAAIHGSIRPIVARYIE
                     SVASQLSDMGFRKELLVMQANGGMMAASVVGEHAVHTVMSGPAGGVLAAAAIAKASGF
                     RNIITGDMGGTSYDVAVVVDGEPVITAEKDLAYGVPIRIPMIDINTIGAGGGSIAQID
                     AAGMLRVGPESAGSFPGPIGYGRGGKDPTITDADLLLGRINPVAITGSDSPAPLDEIA
                     RILEEKIGRRLGLDAVSSAAAIVDIAVNELAGAIRLISIEKGHDPRDFAMMPFGGAGP
                     LHAVAIARELGIPHVLVPRFPGLTSALGCILADVRHDFVQTVNLPLASVDRAEIDRIF
                     AQQVEEGHALLAREKVEVEKVIVRHEADLLYRGQSHVIRLSVESPGFDPEKLIEKLAQ
                     HYRDRFGISLAEMVPMLVNVRTTVIGERESLDLSLFRPQVQGSVEDARTGKRKVFFDG
                     KWMESDIYRRDLLPAGAQFEGPAIVEQMDSTLVVDPGARCRVDDIGNILIDVG"
     gene            7261..8922
                     /locus_tag="VF226_21460"
     CDS             7261..8922
                     /locus_tag="VF226_21460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX7076616.1"
                     /translation="MTTDPVLLTVIEKGLQQVCTEMDIVHEKTAFSPVISEAFDRSNG
                     IYALEDGSMIAQGEIALPIFIGVMQETTRSVIKARDDLEDGDVILVNDPYFGGTHLMD
                     VKMVRPFYYRGERWCYLSNTGHWPDTGGMVPGGFNSTATEIHQEGLRIPPIKLCRRGE
                     IDQEILSLVLANIRVSDERIGDIKAHLGALEAGARRLTALLDRYGRETVEEAIAELRR
                     RSEQMMRAHIETIPDGTYSSVAYLDSDGVVNEPLEVRLEVTVKGSDITFDLSRSSPPC
                     AGPLNSVWATTVSAVYGAMKHIFPNVPMNSGCFAPIHVLEPRGTFLYAEYPRPVAGCA
                     AEVSQRVMEAVFLALAQAIPDRLFAAPAGTSGNISLGGYDPEEKRHYIMYYFSGGGYG
                     GWWRGDGISNGCSTIGISKSQPVEILEQRYPIIFDEFALREGSGGAGKYRGGFGVRYR
                     ARLLRGEGKASFLMEHGRFGPPGILGGKPGAPNVIRVCRSGNIIEPEHISKGEGFVLK
                     PGDWIEVQTPGGGGYGDPAERDPALIERDRRRGYYADRPGTEAVD"
     gene            9050..9757
                     /locus_tag="VF226_21465"
     CDS             9050..9757
                     /locus_tag="VF226_21465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEX7076617.1"
                     /translation="MNKQVQSAAEAVADIRDGATVMISGFGEAGSPIELIHALIDQGT
                     RDLVVINNNAGNGHVGLAALIASGQVRKVICSYPRSTNSEVFQNLYREGRIELELVPQ
                     GTLAERIRAGGAGIPAFYTPTSAGTPLAEGKEVRDFGGRPHVLEYALTADFSLVKAER
                     ADRFGNLVYNKTARNFAPIMCMAARTTIVQVRQMVAAGEIDPEVVVTPGIFVDRVVHV
                     PNPAQESKLVAEGVRYP"
     gene            9754..10440
                     /locus_tag="VF226_21470"
     CDS             9754..10440
                     /locus_tag="VF226_21470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX7076618.1"
                     /translation="MNSKPVAIGLSREAMAWRAAQDIPDGAYVNLGIGIPEMVARFVP
                     AGREVVYHSENGILGFGPPPEPGQEDPELINAGKKPITLQPGTSFFHHADSFAMIRGG
                     HIDICILGAMQVAANGDLANWSTGEGGVPAVGGAMDLVAGVKQVFIITEHTTRKGEPK
                     LLTRCTYPLTGAGVVSRVFTNYGVFDVADGRFIMREVAPGVHVETVQAATDGPLEIAP
                     DLKVIDAPVR"
     gene            10545..11093
                     /locus_tag="VF226_21475"
     CDS             10545..11093
                     /locus_tag="VF226_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX7076619.1"
                     /translation="MKLPFFTIGHSTRTIEEFVGLLQLAKVGLVVDVRTVPKSRTNPQ
                     FNKTTLPDALSEFQIAYEHMPALGGLRGKCKSCRESLNAFWQNQSFHNFADYALSPEF
                     RAGLDQLIERGRVRRCAVMCSEAVWWRCHRRIIADYLIARGETVFHLMGTDRIEPARL
                     TPGAVVHQDGSITYPAAKAATP"
     gene            11237..12079
                     /gene="dapD"
                     /locus_tag="VF226_21480"
     CDS             11237..12079
                     /gene="dapD"
                     /locus_tag="VF226_21480"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX7076620.1"
                     /translation="MTTADLQRTIDAAWESRDGVNSATTGAVREAVESALNLLDSGKA
                     RVAEKIDGEWVVHQWLKKAVLLSFRLNDMTTIAGAPGGASWWDKVPSKFAGMDEAAFR
                     AAGFRAVPGAIVRHSAYIAPGVVLMPSFVNLGAYVDSGTMVDTWATVGSCAQIGKNVH
                     LSGGVGIGGVLEPLQANPVIIEDNCFIGARSEVVEGVIVGEGSVLSMGVFIGASTKII
                     DRSTGQVHTGRVPPYSVVVPGAMPGKPLPDGSPGPSLYCAVIVKTVDAQTRSKTSIND
                     LLRD"
     gene            12089..13264
                     /gene="dapE"
                     /locus_tag="VF226_21485"
     CDS             12089..13264
                     /gene="dapE"
                     /locus_tag="VF226_21485"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX7076621.1"
                     /translation="MALVGTDPVALAQALIRCPSVTPEEAGALSVLEDVLAPAGFTVH
                     RMTFSEPGTPDVENLYARIGAAGPNLCFAGHTDVVPPGDLAKWSVPPFSGEIRDGVLF
                     GRGAVDMKGAIACFVAAAMRYMKGRGGQPQGSISLLITGDEEGPSINGTAKVLEWLKE
                     RGERLDGCIVGEPTNPQAIGDEIKIGRRGSLTGELVVHGKQGHAAYPHMAENPIPKLA
                     RIIDRLSTTPLDQGTPDFEPSNLQVTVISVPNNATNVIPAEARAKFNIRYNDLHRRPS
                     VEAWVREQCAAAAEEVGARWSVTFSGTGDVFLTKPGPLVDTMVEAVRAVTGRVPRLST
                     SGGTSDARFIQAYCPVIEFGLVNQTIHQVDEHVPIGDLEQLTAIYERFIAGYIAAAV"
     gene            13326..13703
                     /locus_tag="VF226_21490"
     CDS             13326..13703
                     /locus_tag="VF226_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7076622.1"
                     /translation="MGHTDIMQRPFVHPAIDDIALESILYALADPVRLAIMLKLTSGG
                     CPMNCSTAAPEKLPKSTQSHHYKVLREAGLIRSERRGTEVVNTLRCHELDAKFPGVVA
                     SILKAAAAQMNREAKKGCEPESV"
     gene            complement(13725..14831)
                     /locus_tag="VF226_21495"
     CDS             complement(13725..14831)
                     /locus_tag="VF226_21495"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX7076623.1"
                     /translation="MSDGAGSGTQANGILPVQAIRRLIAEGQVRLAQPMLQDQLQPAS
                     LDLRLGDCAWRVRASFLPGPNIPISDKLNELALHKIDLTQSAVLETGCVYVAPLLESL
                     ALPSGISAAANPKSSTGRLDVFTRVIADGVSAFDQVPAGYAGPLYAEICPQTFPIIVR
                     QGSRLCQVRFRQGTAADSDAALADLHRRERLVSSDRPDISHGIALSVDLAGDASGLVG
                     YRAKRHTGLVDVDAPGSCAVLDYWEPITLRGEKRLILDPDQFYILASREAVNVPPTHA
                     AEMVPFNPLVGEFRVHYAGFFDPGFGYAPSGGAGSRAVLEVRSHKVPFILEHGQIIGR
                     LIYERMAELPEMLYGAALGSHYQAQGLKLSKHFK"
     regulatory      14948..15024
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            15099..16313
                     /locus_tag="VF226_21500"
     CDS             15099..16313
                     /locus_tag="VF226_21500"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX7076624.1"
                     /translation="MAQRDTVSDRGPSQNWAPETLLVHGGQLRTGFGETAEALFLTQS
                     YVYETAEQAEARFKGTEPGFIYSRFSNPTVSMFEERMRLLEGAEAARATATGMAAVTS
                     ALLSYLSAGDHVIAARSMFGSCRYVVEELCPRFGIAATLVDGGNIEAWRKAIRPNTKA
                     VFFETPANPTLEAVDIKAVSDLAHDVGALIFVDNVFATPLLQKPLTLGADVVVYSATK
                     HIDGQGRCLGGVVLCSEKFLKDHLHTFLRQTGPSLSPFNAWVLLKGLETLPLRVHAQC
                     ATATKIADWLAERPEVSRVLYCGRADHPQAELIRKQMSGGGQVVTFEIAGGKPAAFRF
                     LNALRLIRISNNLGDAKSLITHPATTTHQRIKPEVRAELGISDGMLRLSVGLESADDL
                     IADLAAGLTAAR"
     gene            16621..17298
                     /locus_tag="VF226_21505"
     CDS             16621..17298
                     /locus_tag="VF226_21505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7076625.1"
                     /translation="MIVIVDERETVSDGFASWFDREGVSATGLCPVDFDQWVETVSQR
                     DIMAVEAFLLGDCRERHSLSRKIKGRSGAAVIAMNEKRSLEETLELFAAGVDDVVRKP
                     IHVRELLARIEAIRRRAQRDAVDEGTEAGDLRVFFDGRDPEVKGEPLPLPRRERRILE
                     YLVMNKGRRVTKSQIFNSVYGLFGENIDETVIESHISKLRKRLRQRLGHDPIDSQRYL
                     GYRLIYQ"
     gene            17450..18694
                     /locus_tag="VF226_21510"
     CDS             17450..18694
                     /locus_tag="VF226_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HEX7076626.1"
                     /translation="MMRTGASGMAAQSSRLGTVADNIANMSTTGYKRASTEFSTMVLD
                     PGAGSYVSGGVESHTRYAISSQGPLAYTTSPTDLAVRGEGFFLVSGPGGQIYMTRAGS
                     FVMNGDGQLINAAGYSLMGYPIAGGDPNMVANGFAGLEVINVRDLAMQASPSTEGRFY
                     ANFPAGADIIPAADLPSANAATAAFTGKTSLVTYDNLGGEVIIDIYSSKVAGETWEVT
                     VFDRSAAAAGGGFPYASGPLASVTLTFDPATGKLDAASPTSIGIPIPNGATLNLDLSQ
                     SSQLAADYVVADPVVNGNAPSDVEGIEISEAGELFAVYRNGARIATHRIPLANVTSPD
                     NLQPLAGNVYTATAESGDVRIGFSGAGGLGRIVSGALEQSNVDLASELTTMIESQRNY
                     TANSKVFQTGADLMDVLVNLKR"
     gene            18734..20203
                     /gene="flgK"
                     /locus_tag="VF226_21515"
     CDS             18734..20203
                     /gene="flgK"
                     /locus_tag="VF226_21515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEX7076627.1"
                     /translation="MTLTSAIDVARSGLSVTSGQTAVVSRNIVGAGEALASRKLANVV
                     TAPGGGVRLASISRVSDRALFAHMLASNSAAALQQSIVDALDRLDGTTLDPELDASPA
                     ALIGKLNDAIQLYSATPHDAVAAQSAVAAARDVALALNDATDTIQGLRQQADAEMASA
                     VDRLNVLLTQFEKLNTAIVNGTRAGSDVTDDLDNRDRLLEAISEEIGIRTVLRADNDM
                     LIFTDSGVTLFEKKPRLLSFEPTLIYAPNTIGNAVYADGVQITGTPGGMRISNGRLAG
                     LATVRDDITVRYQSQLDEIARGLIETFAESDQSAPPTLPDAPGLFTFPSATSVPAPGA
                     LVVGLAGQITVNPSVDPAQGGDVTKLRDGGLAGPEYDYNPSGAASFSGRLQEMLENMA
                     TQRPFDPSAGLAASATIAGFAADSVAWLQENRMSASSSLDYRQTVLERSSEALSNATG
                     VNLDEEMTILLGLERSYQATSRLISIIDDMFRALLAAAG"
     gene            20210..21256
                     /locus_tag="VF226_21520"
     CDS             20210..21256
                     /locus_tag="VF226_21520"
                     /inference="COORDINATES: protein motif:HMM:NF004669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated family protein"
                     /protein_id="HEX7076628.1"
                     /translation="MITSVSTRSLFGATRTTLVRAQSALALAQKETTTARHADVGKSL
                     GFMTGHAISFRNDYAQLGAIRDANSIAQTRLSATQSVLEGLAEDAQSFLGSLVGARNS
                     DGGARVLAVEAEARLKSLINGINTAIEGAHIFSGIDSDNTPLEEYFGNPTAASRQAVA
                     DAFTGAFGFAPSDPAVNSISEADMQGFLSGAFADLFEEPSWSAYWSRASDENISSRIS
                     TSEVIETSANANASAIRKLVSVYVMVADLGGERLNDGAFQAIVDTAIEATGAAIQDLT
                     GLRTKLGIAEDRIATANERISIKMDVLTKHITNLEAVDPYEASLRVTSLLIQIETAYA
                     LTARLQQMSLIYHL"
     gene            21281..21631
                     /gene="flaF"
                     /locus_tag="VF226_21525"
     CDS             21281..21631
                     /gene="flaF"
                     /locus_tag="VF226_21525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEX7076629.1"
                     /translation="MSNPSVYAEILEDTPKLARERERAAIGRAVDLLERASVKGPRSR
                     EAVEALDYLNRLWSILIEDLANPENDLPQRLRADLISIGLWVLREVDRIRLQQSGNFS
                     ALVDVMNTIREGLQ"
     gene            21628..22074
                     /gene="flbT"
                     /locus_tag="VF226_21530"
     CDS             21628..22074
                     /gene="flbT"
                     /locus_tag="VF226_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEX7076630.1"
                     /translation="MSGSIQISLRAGERFYVNGAVLRANRKVTLELLNEATFLLEQHV
                     MQAEGATTPLRQLYFVVQTMLIDPAKAEEARSLFEKFHKLLKTSFESAAVLAGLERVH
                     QLVAADRAFEALKTLRVLFPIEDDILAAGQIQAHALPAQTHKAEAV"
     gene            22074..22475
                     /gene="flgD"
                     /locus_tag="VF226_21535"
     CDS             22074..22475
                     /gene="flgD"
                     /locus_tag="VF226_21535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEX7076631.1"
                     /translation="MEVAAAAGSSPTGLTGGTGAKSRAQLDYDAFLQLLIAQMKNQDP
                     TQPMESTEYIAQLAAFSNVEQAVQTNAKLDSLMAALSLSQADGVIGRTVSSADSDISG
                     KVTALRVAEGGTVLILDNGQELPFGPGITIT"
     gene            22478..22744
                     /locus_tag="VF226_21540"
     CDS             22478..22744
                     /locus_tag="VF226_21540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEX7076632.1"
                     /translation="MNQADALDIVQTTIWTVIVAASPAILAAMLVGVAIALLQALTQV
                     QEITLTFIPKIIAILLTASVSAPFIGATIYTFAEQVYGRIEHGF"
     gene            22775..24865
                     /gene="flhA"
                     /locus_tag="VF226_21545"
     CDS             22775..24865
                     /gene="flhA"
                     /locus_tag="VF226_21545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEX7076633.1"
                     /translation="MAATAAPRVTATSRGGGHDIWFAIGIVFILTVLFLPLPPALIDI
                     GLAFSFALSILVLMVALWIDKPLEFSSFPTILLITTMLRLSLNIATTRLILSNGAEGV
                     TAAGHVIAGFASFVMSGDFVIGAIVFVILVTVNFVVITKGATRIAEVGARFTLDGIPG
                     KQMAIDADLSAGIIDDKEATRRRRELEEESSFFGAMDGASKFVRGDAVAGLIITGVNI
                     VGGMVIGIARHDLSPAQAADVFTKLSVGDGLVSQIPALIVSLAAGLLVSKGGTRGSAE
                     KAVLGQLGRYPRALFVAGLVVAVLAIAPGLPFLPFASLAALLTSVALAIPRRQAEAAA
                     EAQAREAVERQKAEDEARSSIKASLKAAEIEVCLGKQISTTLIAAHGELAQRVTKMRR
                     KFAREFGFVVPDIKLSDDLALPPKTYQIKIHGTAVAKAELRIGDMLVIIGDGPRPDVP
                     GEECQEPAFGLKALWVPEALAANVKRDGLTPIDPPTVLLTHLREVIRNNLAQLLSYKD
                     VRALVDGLEPEYRRLADELMPAQISYSTLHAVLKLLLQERVSIRNLHLILEAIAEAVP
                     HIRRVEHLAEQVRIRLAQQICGDLAENGVLKMLRLGNRWDLAFHQALKRDAKGEVVEF
                     DIEPQLIEQFGAEAAKAIRWHMDRGHRFAVVAAPEARPYVRMVIERLYPALPVLSHLE
                     IARGVQIELLGTIS"
     gene            24931..25617
                     /locus_tag="VF226_21550"
     CDS             24931..25617
                     /locus_tag="VF226_21550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEX7076634.1"
                     /translation="MLMPGFSSPRVPVQIRLFVAIAVTLALTPILLPVLKSSVFRPSL
                     SAAFVLIIGETLTGGFIALLVRIYFMALQFMAAALANFVGFYGFSEMGVEESEPNAPL
                     AGLITLAAAVLFFIGDLHWEVLKGIVQSYAALPLQGPLVVEFGLARLAEATADAFLLA
                     LQIASPFLVYALAVNLAFGLLNKLTPQIPVYFVSLPFVIAGGLLLLYFVAGEFLSFFM
                     VAVESRLIRG"
     gene            25733..26017
                     /locus_tag="VF226_21555"
     CDS             25733..26017
                     /locus_tag="VF226_21555"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEX7076635.1"
                     /translation="MGYVPPSPPQMSQRPGVNPAADTAVREFEGFVLQMFLEAMLPEE
                     ASSVFGSGTTGAFWKSMLAEQIAKQLAGAGGIGVGRLIADGIAATSRDVG"
     gene            26017..26376
                     /locus_tag="VF226_21560"
     CDS             26017..26376
                     /locus_tag="VF226_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076636.1"
                     /translation="MSSVGVFVDRVIDHLEQVIQEETSLLKSRQAVDLYEFNNRKSQG
                     LYNLGRALLMLEGRKLESSTLAKLESLRRALTANEAAISVHLHAVREITDVISKAVRE
                     SESDGTYEAPDPLRGKV"
     gene            26373..26837
                     /locus_tag="VF226_21565"
     CDS             26373..26837
                     /locus_tag="VF226_21565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076637.1"
                     /translation="MIKLLALGVWITAVTIASSFAALSWKTGQLPRPGPLGLFEELTT
                     INTRLISIPFIADGAVQGYVVTKLAFAVDTALLKQFSVEPELVLVDEAIRTIYSSSDF
                     DFRQMARQDLSRLAKTLAKNANERFKANLVAEVFIQELNYVTKDMVRNGGNR"
     gene            complement(26846..27571)
                     /gene="flgF"
                     /locus_tag="VF226_21570"
     CDS             complement(26846..27571)
                     /gene="flgF"
                     /locus_tag="VF226_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEX7076638.1"
                     /translation="MQSGLYVALSGQLAIQRRLDTIAQNVANSSTAGFRGEQVKFESI
                     LSQTPLDSVAFSSAGKSYLSRRPGELTRTDNPLDVAIEGDAWFAIATPAGQVYTRDGR
                     MRMTEAGELQTLNGYAVLDVGGAPIVLNPNAGPPQIARDGTITQDGQQMGAIGLFRIS
                     ELSSLARYENSGVLPDRPAEPVLDFVQTGVVQGYVERANVNPIEEMARLVSVTRAFEA
                     ITSALNDTDSSLREALRALGGPS"
     gene            complement(27574..28389)
                     /locus_tag="VF226_21575"
     CDS             complement(27574..28389)
                     /locus_tag="VF226_21575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEX7076639.1"
                     /translation="MGTISWPQGVLVLTTFTSYRLITSDLPRSLAAIARKPQVARETD
                     YYLERIGEVKSIDDFLADNRLFTYAMKAFGLGEMTYAKAFIRKALTEGIDDPQSFANQ
                     LSDQRYRDFVSTFNFARYGTATTSFERTRQGTVDKYVRQMLEEDAGSRNEGVRLALYF
                     QRKAPLLKSAYGILADRALLAVVQTALGLPPATALADIDLQAEMISRRLDIEEFKDPN
                     KLQRFIARFLSLWELTNPGTQANSPAVSAGWRAGVGITPSLLASLQNLQVKGS"
     gene            28525..29406
                     /gene="motA"
                     /locus_tag="VF226_21580"
     CDS             28525..29406
                     /gene="motA"
                     /locus_tag="VF226_21580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX7076640.1"
                     /translation="MTIIIGLLITLGAMLGGFTAMGGHVEVIWQPWEYVIICGAALGT
                     FVVANPMKTIKDVGRGLIEVIGMRTPKPREYLETLGALHDIMRELRGPSRNELEAQIE
                     NPHESKLFRKFPTVLANRQLTTFICDYCRLILMGNARTHEIGALMDEEIETMVHDSLK
                     PYYALVTIAEALPALGIVAAVLGVIKAMGALDQDPEILGSLIGAALVGTFAGIFFSYA
                     VVSPVASKLRAIREKKMRLYVVVKQTLLAFMNGAMPQVALEHGRKAISAFERPSIDEV
                     EQQTVSGGEGGPRREAA"
     gene            29407..30354
                     /locus_tag="VF226_21585"
     CDS             29407..30354
                     /locus_tag="VF226_21585"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX7076641.1"
                     /translation="MSKTATNEGAPGRRNALLEIFCRGAGYSERLPMLPAALERAALA
                     CTDEFGCLSSVRPEMSYLGLESGRAAHLIEREKDAHLVGMLHAPAWDAHLLVSFDRDL
                     IYTYVELVFGGDGTQPPYAEPRPLSRIEMRIAQSLLGRLAGTLSRAFHPFAPTSFAPE
                     GELGRIDLDRLGGPNTPVAIARFKLRARQATGDLLLAIPDAVLTALKPAFARREPPRS
                     GTADPDWAQQIHSEINRSRLTMRAVLEERQRPLSEIAGLRVGQILPLDATPRTPIRVD
                     CNGEPMMWCELGQQNGNYTLRVHAFVDREQEFMDDILFG"
     gene            30563..30862
                     /gene="fliN"
                     /locus_tag="VF226_21590"
     CDS             30563..30862
                     /gene="fliN"
                     /locus_tag="VF226_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX7076642.1"
                     /translation="MPKQARQDQSSGNNIDMVMRIPVSVQVVLGAARMSVSGLVKLGR
                     GAVIPLDRRVGEPVDVMVNGRLIARGEVVVMDEGSSRFGISLTEIVTGPGSARDA"
     gene            30876..31895
                     /locus_tag="VF226_21595"
     CDS             30876..31895
                     /locus_tag="VF226_21595"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HEX7076643.1"
                     /translation="MTKAQAPARPEPKSLSGVEKVTVLLLALGRPKAAQLLKRMDAEE
                     IRLIARAANQLPLISADDLAELVDEFARMFSTGVNFVGTANAVKDLLAEVMTEGEVAD
                     LLSDTGGEEEPIWSRIARLKDEIIRAYLLREHPQTMALILSRLSPPRAARLMGSLPAE
                     LRNSLLIRMLGIKFVAPDQLEILENTLREDLISLQAPSGGTHAGIADILNRLDKAQFD
                     LALKHLTKARPADVEAMRDMLFTFDDLASLPPGTLGVILNQVPPDRLVLALRETDFAF
                     QDAVLSGLPARARRIVELELQTENNESAREIADARRAIVETVLKLVAERRIELPATKV
                     RAAAS"
     gene            31910..33004
                     /gene="flhB"
                     /locus_tag="VF226_21600"
     CDS             31910..33004
                     /gene="flhB"
                     /locus_tag="VF226_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEX7076644.1"
                     /translation="MSEAPDRESRTEEASEKKIRDAVEQGSVPHSREVVTFASLLGIL
                     LILRMSFPGIGRLRAVLERFIDDPGGWSLENGADAVHLLGIVGSAMAKELIPLVATLC
                     AFGLGASLIQNTPRLVADRIRPRLSRLSLSQGWKRIFGAQGRVEFLKASFKLAAVGCT
                     AVFLLRATQADVFNAMLAEPSALPELLRDVSVRLVTGITVATLLLAVADLIWSRVFWR
                     RELRMTRQEIKDELKQTDGDPIIRSRLRSLARDRSRRRMMAAVPRATLVIANPTHYAV
                     ALRYVREEGGAPRVLAKGRDLIALRIREIAEQHNIPVIEDKPLARALHDKVGIDQMIP
                     PEFYKAVAQIVYFVMTRKGGHARGGLGLTK"
     gene            complement(33163..34638)
                     /gene="gatB"
                     /locus_tag="VF226_21605"
     CDS             complement(33163..34638)
                     /gene="gatB"
                     /locus_tag="VF226_21605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX7076645.1"
                     /translation="MQDRAKAGKSSNLIAGATGDWEVVIGMEVHAQVNTRAKLFSGAS
                     TEFGGEPNSHVSLVDAAMPGMLPVINEECIAQAVRTGLGLKAQINLKSVFDRKNYFYP
                     DLPQGYQISQYKQPIVGEGEVEIDADGETITVGIERLHLEQDAGKSIHDQHPDYTYVD
                     LNRSGVALMEIVSKPDMRSARQAQAYVSKLRTILRYLGTCDGDMEKGNLRADINVSVR
                     RPGEPFGTRCEIKNVNSIRFIGQAIEAEARRQIDILEDGGEIVQETRLFDANKGETRS
                     MRTKEEAHDYRYFPDPDLLPLELTQEWVDALKADLPELPDEKKRRFMEQFGLSSYDAG
                     VLVAERASADYFEKVASGRDAKAAANWVINELFGRLNKEGLDIEHSPVSADQLGGILD
                     LIASKTISGKIAKDVFDIVWTEGGDPADIVEKRGLKQVTDLGAIEQAIDKVLAANPDK
                     VEQVKAKPTMLGWFVGQVMKATGGKANPAAVNELLKQKLGI"
     gene            complement(34776..35060)
                     /locus_tag="VF226_21610"
     CDS             complement(34776..35060)
                     /locus_tag="VF226_21610"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX7076646.1"
                     /translation="MRNRQLLGFKFRRQVPIDRFVMDFVCFDAKVIVEVDGATHSTQD
                     EVMRDTERTCALESFGYLVLRFRNVDVYENVDGVLDVLARTLQLRGAPSP"
     gene            complement(35131..35457)
                     /locus_tag="VF226_21615"
     CDS             complement(35131..35457)
                     /locus_tag="VF226_21615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX7076647.1"
                     /translation="MSTRRPEDCTDMSQVRAEIDRLDAALVDLIAERFGYVERAWQLK
                     LAAKQQANVPWRNQQVIDRVRARAAERGVPPDLCEALWRQMIGWFIQYEEEKLRNELE
                     AKAKEG"
     gene            complement(35471..36958)
                     /gene="gatA"
                     /locus_tag="VF226_21620"
     CDS             complement(35471..36958)
                     /gene="gatA"
                     /locus_tag="VF226_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX7076648.1"
                     /translation="MTDLTRLTLAEAREGLKAKSFTAKELAQAFIDAIEAANPALNAY
                     VATTADRALAQAADSDARIARGEARPLEGLPLGVKDLFCTRGVHSQACSHILDGFEPT
                     YESTVTQNLWDAGAVMLGKLNMDEFAMGSSNETSYYGPVISPWRRNNGSNAKLVPGGS
                     SGGSAAAVAADLCLAATATDTGGSIRQPAALTGTVGIKPTYGRCSRWGIVAFASSLDQ
                     AGPIAKTVRDAAIMLKFMASTDPKDSTSVDAPVPDYEAEVGKSIKGLRVGVPKEYRME
                     GMGAEIEELWEKGIAWLRDAGASIHEISLPHTKYALPTYYILAPAEASSNLARYDGVR
                     YGLRITGDDLIDTYERTRAEGFGKEVRRRILIGTYVLSAGYYDAYYLKAQKVRTLIKR
                     DFDEAWDKVDVVLTPTTPSPAFALGEKSGDPIAMYLEDIFTVTVNMAGLPGISVPGGL
                     SRQGTPLGLQLIGKPFDEGTLFRAGQVIEDAAGRFTVPDRWWLKG"
     gene            complement(37119..37406)
                     /gene="gatC"
                     /locus_tag="VF226_21625"
     CDS             complement(37119..37406)
                     /gene="gatC"
                     /locus_tag="VF226_21625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX7076649.1"
                     /translation="MEVDEATVRRIARLARIKITDEEAQGLQSELTGILNWVEQLNEI
                     DTSNVEPMTRVVPISLKKRKDEVTDGNIADDIVKNAPMTDDHFFVVPKVVE"
     gene            complement(37523..38695)
                     /locus_tag="VF226_21630"
     CDS             complement(37523..38695)
                     /locus_tag="VF226_21630"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7076650.1"
                     /translation="MARRSRLLIIGLPLVALAAAAGAAVSITRSQPVEVTLVPAISPP
                     VQPSTAAGAAGGPARGFIGAAGLIESNGQEIEIGSHTSGIVSEVLVKPGQKVKAGDPL
                     FVLDERAARGSLALRRAELAAAERQRDQILATEATLKAQRAAAIAAANAARAEMEEWA
                     DQVRVAEALKARGGNAISDREVTHRRNAERSAEGRLEEAQARIAQIEAELKLITPPDG
                     ASLAVATAAVEQARRAVERAEIDLDLLTVRAPINGTVLQVNVRPGEFAFAGTLATPLI
                     VLGNLDPLHVRIDIDEADVPRFEPSAPAYASLRGLASERMRLSFVNIEPLLVPKRSLT
                     GASGERVDTRVLRVIYALPAGTKHAYPGQQVDVFIESTPIGSTQVTRSSIDTGALP"
     gene            complement(38696..39442)
                     /locus_tag="VF226_21635"
     CDS             complement(38696..39442)
                     /locus_tag="VF226_21635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076651.1"
                     /translation="MLKLAREITQASTAPNLAVRCRGIVKEFGSGETRIRVLHGIDLD
                     IETGSMTYIVGPSGCGKTTLISVIAGILSAEEGEVELFGTRLGALSAARLAQFRAETI
                     GFIFQQFNLLPALTAAENASVPLLVNGASESRALRKARELLAAVGLEQHMDKFPAELS
                     GGQQQRVAIARALVHEPRLVISDEPTASLDAQAGQQAMELLRQHALRPDRAVIVVTHD
                     NRIFHFADRIARMSDGRITGIELPPFTEGH"
     gene            complement(39447..40592)
                     /locus_tag="VF226_21640"
     CDS             complement(39447..40592)
                     /locus_tag="VF226_21640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076652.1"
                     /translation="MNRVALKMLFGDAAKLLGLVFGMAFSTLLISQQASMFIGVMQRT
                     ANLITEIREPNIWVMDPAIEQIDAVRPMRDTELGRVRGVPGVAWAVPFFKGQAVVTTE
                     TGGLQSSFVYGLDDVTLIGLPPEIILGDRESLRRPDAIAIDEAGYKKLWPTQPLTLGQ
                     TLELNDRRAVVVAILRTAPPFQTQPLIYTRYSQALVFTNNGRNQLSFVLARSAPDADP
                     AAVAQAIEERTGLKAVTSDAFRWETMMYFLRHTGIPVNFGTVTLLGVIVGIAVVGLTF
                     HMFVIENLKQYAALKAMGLTNGRLIGMVLLQATIVGAIGYAIGLGLTSVFFEAVTSNP
                     TSFFRGFYLPWQVAVGVGLLATAIMLLSAIVSLRRVLVIDPAIVFRG"
     gene            complement(40603..41235)
                     /locus_tag="VF226_21645"
     CDS             complement(40603..41235)
                     /locus_tag="VF226_21645"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7076653.1"
                     /translation="MSTRKRRKEQRPEEILNAALEEFIAKGYAGTRLDDVARRAGISK
                     GLVYVYFDTKEDLFEAVIRTFLVPHVEMLQGEIEGSQLSSEALLRGPVLGLMKKIVNS
                     RVHELVRLLIAEGQRHPDLTAFYYEEVVSRGMSVIRTIIDRGIARGEFRETPLREFPQ
                     LVIAPMLMAMVWKLLMERHRPLDAERILETHVELLLQFLKTGNAPPDTTA"
     gene            41440..42681
                     /locus_tag="VF226_21650"
     CDS             41440..42681
                     /locus_tag="VF226_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076654.1"
                     /translation="MIDALLPTALAGRHARRAAGPLIVACFSAVFLIALPSGSADAAD
                     RVNAMIERLDASERRIFLEWRAAQKKHEAALDAYWGKVQSTRSQRRKKIRAGRPVSAD
                     DYVMTFPPEYSGPKLPSHIARIWDELKPPVERKPVPSVADVLESAKAVYGFVPERIPE
                     KEFKRRYALEALANGLSREQVVRVYALETGGRGTYDMQAGINPSTGRGTPISTALGYA
                     QLLHANSVNELVKHGPTFIARLEKTAETPGIAPERAAALQKKARVLRRMLASAKKVPN
                     DWYAHMRFAATRNGLGIHALNLDGDIGPMLQVIKLKGVRQVAERAGRTDLAPAELELM
                     NLAGPSTGLEMMTPIGRTAPTPNFFSRLGYERNPVVHKRTAAELLEKLESIMDANVKK
                     PGSIEFAAAFDEAMSIRSSQR"
     gene            42948..>44625
                     /locus_tag="VF226_21655"
     CDS             42948..>44625
                     /locus_tag="VF226_21655"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7076655.1"
                     /translation="MSQFVFAVIAIALVFLLAMRRASLPVWAGVATAMTFAWQIGLAG
                     GGPSAFGFWSLVAWLPALALAVLSVPEVRRSLVVEPAYRAIRSSLPKISETEQQALEA
                     GTIGFDAELFSGRPDWNRLRAVQPIVLTAEEQAFLDGPTEELCRMIDDWRIRTREREI
                     PEEIWSFVKEKGFLGMLISKQHGGLGFSAQAQSLILGKISSRSPDVATIVMVPNSLGP
                     GELIEKYGTPEQKSHYLPRLARGEEIPCFALTGPTSGSDAASMRDVGYVTRGVHEGRE
                     TLGIRVIWEKRYITLAPKATLLGLAFRLFDPENLLGKGEDVGITLAIIPTSHTGVNIG
                     RRHLPCGAAFPNGPTWGKDVFIPIDWIIGGQARAGQGWRMLMECLSAGRSISLPSSSA
                     AGIKTMLRFSTAYGRIRRQFGLPIARMEGVEEPLARLVENAYLVEAGRAVTAAMVTRG
                     EKPAVLGALMKYQSTERMRQAVNDAMDVHGGRGICDGPTNYLQSAYQMVPIAITVEGA
                     NILTRTLITFAQGALRSHPYLYRELQAVQNPDPKRGLELFEPIFLDHVSFS"
BASE COUNT         8309 a        13473 c        14028 g         8815 t
ORIGIN      
        1 tcgagaccgg cctggatgct ggcctcatcc tccttcgaga tgatgccctg ggcggcgagc
       61 atcgccgcat gcgcctttga gccacggata tcctgggggg cgagagcttt gtcgaagccg
      121 atggaggcgt taatctcctc catgatctcg gccggtgaca tggcgaaccg gcctccccac
      181 attttgttgg ccggaccgtt ggccgagttg tcgtccatgg atcggggctc ccggttcagg
      241 ttgcaacgcg ttcgctcata gagaaagggc cgagcaagct atgttgaact cgacatccgc
      301 cagatccggg ctagtggtgc tcgcggccgc gctggtcgga ttcggcgcgg tatacgtcac
      361 cctcgggcgg aatgacaacg tcgagacgac gacgcctgcg tctcaagccc cggcggctgt
      421 cccgcagaag ctgccggcgg gccctggcac caatgagctg agcaagggcc atatggctgc
      481 cttcgtgttc aagaaggcac ccgagccgct gcccgagttc acctttatgg acggctcggg
      541 cgccgagcgt actctgaagg attggcaggg cagagtggtt ctcctgaacc tctgggcgac
      601 atggtgcgca ccctgccgca aggagatgcc tactctcgaa cgcctggagc aggccctcgg
      661 gtcagacaaa ttccaggtgt tagcgctgag cgtcgatcgc ggcggcgccg aagcatcgaa
      721 aaagtttttt gatcagatca gcgtcgaagc gctggacctc ttcgtcgatc ccactggccg
      781 cgcaacctct caactcaagg ccgtcggcct gccgacaacc ctgctgatcg acgcccaagg
      841 ccgcgaaatc ggccgcctca cgggccccgc ggaatgggac agcgaggatg ccaagcgcct
      901 gatccaagca gtcctaggct cctcctgacc cctttttcga ctatctctcc ggtcattccc
      961 cgatcagctc gcctcatcga tcgctgcttc gcctgaccag cagcgccagg cgggggttag
     1021 tgtggctgca tgtgccggga tcgtcctgtt tgcccctgct tcagcaagga atctgctgcc
     1081 cggggggccc gttgacacta ttgttggggc atgacacagt ttcgagaaaa gaaatcgagt
     1141 atcgaaaatc gaaactggaa aagtgcgtcg tccggagctc ttcgcgggca ttcgcggatg
     1201 attgagctag cccgctgcta acggtgggtg ggggccggga gcttagcgtg tctgaagcag
     1261 ggagaggtgt gcttatgaga ctggtacgta aactggcgcg tttcgttgcg tcgggcgtga
     1321 ttcttgctgc ggctggcgtt gctgcaaacg cggccgaatt caactggcgt attcagagca
     1381 atctcaaccc tggagagccg ggctacgtgg cggtcgaaga gaaattcgcc aagctcgcgg
     1441 aggagatgtc gggtgggcgc atcgctttcc aggttttccc agtgggggct ctttttccgg
     1501 tacaggaagg gctggaggcc gtcggctccg gcatagtgga gatgggcatt ctcaccggcg
     1561 gctatttcgc tggcaagatc gggccgatag cgaacctcga gaacggtgtc ccaggatctc
     1621 tgcggacccc aatcgagcgc tataactttt tctacaagta cggcttcctt gatttggtcc
     1681 gcgaagcata cggcaagcac ggcatatttt atctcgggcc gcagctctcc ccgccgtggg
     1741 acattgtctc aaagaagcca attacatcag ctgaagactt caaagggctg aaaattcggt
     1801 ccttcgggtt ggaagcgaag tggtacgaga agatgggcgc aacgccggtc ttcatggggg
     1861 gtggtgacat ctacaccggc ctggctactg gcgcgatcga cgccgcacgc tgggctagcc
     1921 cgtcagggaa caagaacaac tcctaccatg aggttgcgaa gtattacgtg cagccttcgc
     1981 cgatgccggt tccgaacaac ttcttcgcgg ttaatatggc tgcctggaat cagttgccca
     2041 acgacatcaa ggcaatcctg aacgaggcag ccgttgcatc ctcttttgac tacatcgctc
     2101 gcagcgccat gactgatgcc aaggcgatgc gagacatgca ggccgccggc gttcagattt
     2161 ctgtcattcc agaagacgaa tgggccaaga tggaaagcgc cgcgcgtgag ctttgggaag
     2221 cttatgccga ccaggacgag ttcgccgcga agggcgtcga tctgctgaag aagttcctgg
     2281 ctgagctggg acgctaatgc ctgcacaggc tccggcggga cgcgccggag ccgctccgct
     2341 gagatgatct gctttcctga tgaagctcac cacgagccgc cgcgcagccg cgcatgagcg
     2401 gtggctcttt tccgcaatga aggtcacgat gctagagcga ctcattggct ggatcgacac
     2461 tgcagttgcg gcaatcggca aaggcgcctc ctttctcacg ctcctcatga ctgcgctgat
     2521 cgtccttgag atggtttcga ggagcttttt cggcacgtcc tggggatggg tttacgatct
     2581 cagcggctgg ctcctcgccg cgtatatttt tttgggcggt gcctggactc ttcagcgagg
     2641 ccagttcgtt cgggtcgata ttctgttcac acgtttttcg cctcgcacga gggctctcgt
     2701 tgaccttgtg gctggtacgg cactgtttgt cctgttcgct ggtgcgctgc tgtggctggg
     2761 gactcgcttc gcatggcagt cgttcctgat gaacgaggtt tccgcgacgg gtgcatggga
     2821 tgcaccggtc tacgtagcca aggcgctcct gccgatcggc gttgtcctgc tttgcctggc
     2881 ctggctgtcg cacatcctga agctttggcg aaatggcctg cggagccccg agtagccatg
     2941 gatcccgttc tcatcacggc gctcctcttc ggtagcatgt tcctgtgcct ggcgatcggc
     3001 gtgccgattg cgatagcgct cggtggactg accgtagcga tgatctacgg cttttggagt
     3061 ccgaacgcgc tcaccatgct gccgataaag gccttctcca gcgcgtcgag ctttgaattt
     3121 ctcggcattc cgcttttcat tttcatggca gcgatgctgc agcgctcgaa aatcgccgat
     3181 gacctctatg actccatgta ccggtttttt ggcggagtgc gtggaggact tgccatagga
     3241 actgtcgcga tttgcacgtt gttcgcggcg atggccggga tctcgggtgc tgcgacgatt
     3301 tccatgggct tgctggcaat tccggccatg ctggcgcgga actatcagaa gggcattgcg
     3361 ctgggctgcg ttgcggctgg aggctcgctc ggcatcctca tcccacccag cgtgacaatg
     3421 atcatctacg gcctcatcgc caatacttcc gttggcaagc tctacgccgg cggagttctc
     3481 cccggccttt tgatcgcgct tttgttctgc gtttacatct atgtccgcgg cgttattcag
     3541 ccggaggtgg tgggcagcac gctcgagcga tttacgtggc gagagaaact cgctgctgtc
     3601 aaagggcttg ttctcccgat cttgctcgtc gttaccgttt tgggctcgat gctcggtggg
     3661 tacgcaagcg tctcggaggc ggcggcagtt ggctcgttcg gttcgctcat tgccgctgcc
     3721 gtacgtcgtc aactcaactg gaccacggtg cgtgaggcct gcgaggaaac gattctccta
     3781 agctgcctta ttttttggat cattatcggg gcttcggcgc tgtcgacctt ctacacggcc
     3841 atgggcgcga cccggctcat tgagagtatg attctcgggc tagaagtaaa tcggtacatc
     3901 atactcgcgg gcatgcagct cattttgctg ctgctcggga tggtcctgga tacggtgggg
     3961 atcatcatga tcaccgttcc ggtatttgtg cccattgccc tgaagctcgg atttgatccc
     4021 gtttggtttg ggattctctt catcatcaat atggagatcg ggttcctgac acccccattt
     4081 ggctacaacc tgttttattt gaaaggcgtg gtgcctccga gcataacact tggtgatatc
     4141 taccggtcgg ttacgccgtt catcctgttg atgattgctg ccgttggaat atgcatgctt
     4201 ttcccgcaga ttgtgctggt gctgccgaat gcgttgttct gacagccgga ctacttctcc
     4261 agtgcctgta gcgcgcgcgc aaactggtca gcggcctctt tgaggcctga aatatagaac
     4321 tcaacagggt tctggagcag gcggctgcag gggccggtta ccccgacgct gaacagaacg
     4381 cccgcatcgg gcaaccggac tggcacggca taagccatga cgttctcatc gatctcccgg
     4441 tcgcaaatgg catacccacg cgtgcgtgtt tcttccagtt cggcgaggta ggccgaaatg
     4501 tcagttttgg tgaatggtgt cagccgagct agcggctcgc ggatatagcg cgtgacggcc
     4561 ttctgcggct gatgcgcaag gatcgccttc gccgaagcag ccgcatgcgc tggaaggtcg
     4621 gagcctgggt agacgtgtag ccgatagccc tgatctggcg tggcacgggc taccgtccgc
     4681 acatcggtcc gcccgaggcg cacgacatag caggtctcct tgaggcgacc ggcggcggaa
     4741 tcgcacacca tttgcgcgta ggcggtcaat gcatcccgat caaaaccgag gtagagaatc
     4801 cgccagagtc gcggtccaat gcgaaaccgc ctctgccggt ggtcctcggc gacgagggcg
     4861 cctgaatcga gcagcacacg tgtcagccga tggaccgtgg gcttgggcaa gtcgatgatt
     4921 ttggcaatgt ccgtcatcgt caggccgtca tgggccgcgg cgacgatttc gagaatgctc
     4981 aaatagcgtt cgaacggacc cttccggtcg tcatcacgcc agacctcgtt ggggtggtcc
     5041 atctctccct cgtgctcatg atgctgttgg ccgaagctgg ttgacaacgg cccgggctcc
     5101 gcgtgtatta tcgaaaaata gaatatagtt tcgtttttcg aaactcaaga cttacctggg
     5161 atcgagcgga tactgcggga ttcggaggcg agcgtgagta ccatcagcat cggcgtcgat
     5221 gtcggaggga cgtttactga cttcctcatg atcgacacgg cgcgccgcga ataccgggtc
     5281 gccaaggtcc cgaccaccgt cgatgatcgc gcccgcggtt tccttcaggg actgacggag
     5341 cttgatgccg acccgaaggc catcggctgg ctcgttcatg gcacgactgt cggcacgaac
     5401 gccatcctcg aacggaaagg agcccgctgc gggctgatta cgacacgcgg attccgggat
     5461 gcgcttgagc tggggcgtcg cacgaggccc tatgcctacg gtctgtcggg gacattcgaa
     5521 ccgctcattc ctcgggaggc gcgcctcgag gtgcgtgagc gtttggacgc ggccggtcgg
     5581 gtcatcgagc ctctcaacga ggatgacgta cgcgcggctg tgcagaagct cctggaaatg
     5641 gatatcgaat ccgtcgtgat ccatttcctg cattcctatg ccaacgatgc gcatgaaaag
     5701 cgctgtgccc aaatcgtccg aagcctgtgg ccgaacgcaa acgtcatagc tgggcatgag
     5761 atcatccgcg aaatgcgcga attcgagcgg ggatctaccg cggcaatcca cggatcgatc
     5821 aggccaattg tggcgcgcta catcgaaagc gttgcgagcc agctctctga catgggcttc
     5881 cggaaggaac tgctcgtcat gcaggcaaat ggcggcatga tggctgcctc tgtggtgggc
     5941 gagcatgccg tccatacggt gatgtcgggc cctgccggcg gcgtgcttgc ggccgctgcc
     6001 atcgccaaag cttccggatt caggaacatc attactggcg atatgggcgg caccagctat
     6061 gacgtggcgg tcgtcgtcga tggtgagccg gtcattacgg ctgaaaagga tcttgcctac
     6121 ggcgtcccca tccgtatccc gatgatcgat ataaatacga ttggagcggg cgggggcagt
     6181 atcgcgcaga tcgatgcagc gggcatgctg cgagttggtc cggaaagcgc tggctcattc
     6241 cccggtccga tcggctatgg ccgcgggggc aaggatccga ccattaccga cgccgatctt
     6301 ctcttgggcc gaatcaaccc cgttgccatc acgggctcag actcgcctgc tccgctcgat
     6361 gaaatcgcgc gcattctgga ggagaagatc ggccgtcgtc ttggcctcga tgcggtttcc
     6421 agcgcggccg ccattgtcga tatcgccgtc aacgagctgg ccggcgccat tcgcctcatt
     6481 tcgatcgaga aggggcacga tccgcgagac tttgcgatga tgccgtttgg cggagcaggg
     6541 ccgctccacg cggtggcgat cgcgcgcgag ctcggtatcc cgcacgttct cgtaccacgc
     6601 ttccctggtc tgacttcggc gctcgggtgt attctcgccg atgtccggca cgactttgtg
     6661 cagacggtca atctgcctct cgcctctgtt gatagagccg aaatcgatcg gatttttgcc
     6721 cagcaggtcg aggagggcca tgcccttctc gcccgcgaaa aggtggaggt cgaaaaggtc
     6781 atcgtccgac atgaagcgga tctgctgtac cgagggcaat cgcatgtgat ccgcctgtct
     6841 gtcgagagcc ccgggttcga ccccgaaaag ttgatcgaga aattggcgca gcattatcgc
     6901 gacaggtttg gcatctccct cgcggagatg gtgccgatgt tggtcaacgt gcgcacgacg
     6961 gtcatcgggg agcgcgaaag cctcgatctt tcgctgttcc ggccgcaagt ccaaggcagc
     7021 gtggaagatg cccgcacggg taagcgcaag gtctttttcg acggaaagtg gatggagtcc
     7081 gacatttatc gccgtgacct gctgccggcc ggagcgcaat tcgaagggcc tgccattgtc
     7141 gagcagatgg attcgactct ggtggtggac cccggcgcgc gctgcagggt cgacgacatc
     7201 ggcaatattc tcatcgacgt gggctaaggc ccggctgctg gctgaaggat agggtgattt
     7261 atgaccacag atcccgtctt gctgaccgtc atcgaaaagg gcctgcagca ggtctgcacg
     7321 gaaatggaca ttgtccacga gaaaacggct ttttccccgg tcatctccga agcctttgat
     7381 cggtcgaacg gcatttatgc actcgaagac ggatcgatga tcgcgcaggg agaaatcgcc
     7441 ctgccgatct tcatcggcgt catgcaggaa acgacgcgct cggttatcaa ggccagggac
     7501 gatctcgagg atggcgatgt cattctcgtg aacgacccct atttcggcgg gacgcacctc
     7561 atggacgtga aaatggtgcg gccattctat tatcgcggcg aacgctggtg ctatctctcc
     7621 aacacggggc attggcccga caccggcggc atggtgcccg gcggcttcaa tagcaccgcc
     7681 acggaaatcc atcaggaagg tctgcgcatt ccgccgatca agctgtgccg gcgcggggag
     7741 atcgaccagg agatcttgag cctcgtgctg gcgaatatcc gcgtttcgga tgagcgcatc
     7801 ggtgacatca aagcgcacct cggagcgctg gaagccggcg cacggcggct gaccgcactg
     7861 ctcgaccgct atggtcgcga aactgtcgag gaggccatcg ccgagctgcg ccgccgttcc
     7921 gagcagatga tgcgcgcgca catcgaaacc attcccgacg ggacttactc cagtgtcgct
     7981 tatctcgata gcgacggcgt cgtgaacgag cccctggagg taaggcttga ggtcacggtc
     8041 aaggggtcgg acatcacctt cgatctttcg agatcgagtc cgccttgcgc gggtccgctc
     8101 aattccgttt gggcgacgac ggtttcggcg gtctatggcg cgatgaagca catttttccg
     8161 aatgtgccga tgaactcggg atgtttcgcg cccattcacg tcctggagcc gcggggcact
     8221 tttctttacg cggaataccc gcgccctgtt gcaggttgcg cagcagaggt gtcccagcgc
     8281 gtcatggagg cggtgttcct ggcactcgcc caggctattc ctgaccggct attcgcggcg
     8341 cccgccggga caagcggaaa catcagcctc ggcggttatg atcccgaaga gaagcggcac
     8401 tacattatgt actacttttc cggcggcggt tacggcggct ggtggagagg tgatggcatc
     8461 agcaacggct gctccacgat cggcatttcg aaatcgcagc cggttgagat cctggagcaa
     8521 cggtatccaa tcattttcga cgagttcgcg ctgcgggaag gctctggcgg cgccgggaag
     8581 taccgtggcg gtttcggagt acgttaccgc gcgcggctgc tgcggggcga gggcaaggcc
     8641 tcattcctca tggagcacgg ccgtttcggg cctccgggga ttctgggtgg caaacccggt
     8701 gcgccgaacg tcatcagggt ctgccggagc ggcaacatca tcgagccgga gcacatctcc
     8761 aagggcgaag gtttcgtcct gaagccgggc gactggatcg aggtacagac cccaggcggc
     8821 ggtggctacg gcgatcccgc ggagcgggac ccggcactca tcgagcggga taggcgacgc
     8881 ggttactatg cggatcggcc gggaacagag gcagttgact gaccgctgga tgagaagagg
     8941 gcggccggtc gctgcgcaag taatcttctc gttggccgcg ttgcacgaga tggctctgtc
     9001 ggttatcggg tgatgtgggg cttcaggtga ttgggaacgg cctgtcatga tcaacaagca
     9061 ggtgcagagc gccgctgaag ccgtcgcgga tatccgggac ggggcgactg tgatgatcag
     9121 tggcttcggt gaggctggga gccccatcga gctgatccat gccctcatcg atcagggcac
     9181 gcgcgatctc gtcgtcatca acaacaacgc aggcaacggc catgtggggc tggcggcact
     9241 catcgcctca gggcaagtcc gcaaggtgat ctgctcttat ccgcgctcta ccaattcgga
     9301 ggtgttccag aacctctacc gcgagggccg catcgagctg gagctcgtgc cgcaagggac
     9361 actggccgaa cggattcgcg cgggcggcgc cggcataccc gcgttctata cgccaacatc
     9421 tgccggcacg cccttggccg aaggcaagga ggtgcgcgat ttcggcgggc ggccccacgt
     9481 gctcgaatat gccctgacgg ccgatttctc gctcgtcaag gccgagcggg cagaccggtt
     9541 cggcaatctc gtctacaaca agacggcgag gaatttcgcg cccatcatgt gcatggcggc
     9601 gagaaccacc atcgtccagg tccgccaaat ggttgccgcc ggcgagatcg atcccgaggt
     9661 cgtggtcacg cccggcatct tcgtcgaccg ggtcgttcat gttcccaatc cggcccagga
     9721 atcgaaactg gtggccgagg gagtgcgcta cccatgaaca gcaaacccgt tgcgatcggc
     9781 ctcagccgcg aagccatggc ctggcgtgcc gcccaggaca ttccggatgg cgcgtatgtc
     9841 aacctcggca tcgggattcc ggagatggtg gcgcggttcg taccggcggg gcgcgaagtc
     9901 gtctatcatt cggaaaacgg cattctggga tttggcccgc cgcccgagcc ggggcaggag
     9961 gacccggagc tgatcaatgc cggcaagaag ccgatcacac ttcagccggg cacgtcgttt
    10021 ttccaccacg ccgacagctt cgccatgatc cgcggcgggc acatcgacat ctgcattctg
    10081 ggtgcgatgc aggtggcggc caacggcgat ctggcgaact ggtcgacagg cgagggcggt
    10141 gttccggccg tcggcggtgc catggacctt gtggctgggg tcaagcaggt cttcatcatt
    10201 acggagcaca cgacccgcaa gggcgagccg aagctgctca cgcgctgcac ctatccgcta
    10261 acaggggcag gcgtcgtctc tcgcgtcttc accaactatg gcgtgttcga tgtggccgat
    10321 gggcgcttca ttatgcgtga ggtcgcgcca ggggttcacg tcgagacggt gcaggctgcc
    10381 acggacgggc cgctcgaaat cgcgcctgac ttgaaagtca tcgacgcccc tgtccgctag
    10441 tagaacctgg agagaattca ctaagcgata gggaggggaa ccgagggcgg ctacatgaat
    10501 tccggccgaa acacgagcaa tcgatgtcgc cggcggggac cgagttgaaa ctgccgttct
    10561 tcaccatcgg ccattcgacg cggaccatcg aggagttcgt cgggttgctt caactggcca
    10621 aggtcggcct ggtggtcgat gtgaggacgg tgccgaagtc gcgcaccaac ccgcagttca
    10681 acaagaccac ccttcctgat gccctttccg agttccagat cgcttacgag cacatgcctg
    10741 ctctgggcgg gctacgcgga aaatgcaaaa gctgcaggga aagtctgaac gcgttctggc
    10801 agaaccagag cttccacaac tttgccgatt acgcgctgtc gccagaattc cgcgcgggcc
    10861 tcgaccaact catcgagagg ggtcgggtcc ggcgctgtgc cgtgatgtgc tcggaagccg
    10921 tgtggtggcg ctgccaccgg cgcatcatcg ccgattatct gatcgcgcgc ggcgagacgg
    10981 tgtttcacct catgggcaca gaccgtatcg agccagcccg actcacgcca ggtgcggtcg
    11041 tccaccaaga cggatcgatt acctatcccg ccgcgaaggc cgcgacgcct tagcattcgc
    11101 cgagggtaca ccagcgcctc gagcggtttc tcgatctcga ttacccggtc accgatccgc
    11161 ttgacgccgc acggggtccg ggccacaacg cgggccgtcc agcatcgcca acgaaagcgc
    11221 gcgcggaggc ccgttcatga ccactgcaga cctgcaacgc accatcgacg ctgcctggga
    11281 gagccgtgac ggcgtgaatt cggccacgac cggtgccgtg cgcgaggccg tcgaaagcgc
    11341 gctcaacttg ctggacagcg gcaaggcgcg ggttgccgag aagatcgacg gcgagtgggt
    11401 cgtgcatcag tggctcaaga aggcggtgct gctgtcattc cgtctgaacg acatgacgac
    11461 gattgccggt gcgcccggcg gggcgagctg gtgggacaag gtcccctcca agttcgccgg
    11521 catggacgag gcagcgttcc gcgccgccgg cttccgcgcc gttcctggcg cgatcgtgcg
    11581 ccactcggcc tatatcgcgc cgggggtggt gctgatgccg tcgttcgtca atctgggagc
    11641 ttatgtcgat tctggcacga tggtcgacac ctgggcgacc gtcggctcgt gcgcgcagat
    11701 tggcaagaac gtgcacctgt cgggcggtgt cggtatcggc ggcgtactgg agccgctgca
    11761 ggccaacccg gtcatcatcg aggacaattg cttcatcggc gcccgctcag aggtcgtcga
    11821 gggcgtcatc gtcggcgagg gctcggtgct ttcgatgggc gtgttcatcg gcgcatcgac
    11881 caagatcatc gaccgatcga cgggtcaggt ccataccggc cgcgtgccgc cctattccgt
    11941 cgtggtgcct ggcgcgatgc ccggcaagcc gcttccggat ggctcgcccg ggccgtcgct
    12001 ctactgcgcc gtgatcgtca agacggtgga cgcgcagacc cggtcaaaga cctcgatcaa
    12061 cgatcttctc agggactgat ttgccggcat ggccctggtt ggcacagacc ccgtcgcgct
    12121 ggcgcaggcg ctgatccgct gcccgagcgt cacaccggag gaggctggcg cgctatcagt
    12181 tctcgaagac gtgctcgcgc cggcagggtt caccgtgcac cggatgacgt tctccgagcc
    12241 cggaaccccg gacgtcgaga acctttacgc gcggatcggc gccgcagggc cgaacctttg
    12301 ttttgcgggc cacacggatg tcgtgccgcc gggggatttg gcgaagtggt cggtgccgcc
    12361 gttttccggc gagatccgcg acggcgtgct cttcggccgc ggtgccgtgg acatgaaggg
    12421 cgccatcgcc tgcttcgtgg cggcggcgat gcgttacatg aaagggcggg gcggccaacc
    12481 ccaggggtcc atcagcctgc tcatcacggg ggacgaggag gggccgtcga tcaacggcac
    12541 cgccaaagtg ctggaatggc tgaaggagcg cggcgagcgg cttgatggct gcatcgtcgg
    12601 agagccgacc aacccgcaag cgatcgggga cgagatcaaa atcggacggc gcggcagcct
    12661 caccggcgag ctggtcgtcc acggcaagca ggggcacgct gcctatccgc acatggcgga
    12721 gaaccccatt ccgaagctcg cccgcatcat cgaccggctg tcgacgacgc cgctcgatca
    12781 agggaccccc gatttcgagc cctcgaacct gcaggtgacg gtcatctcgg ttccgaacaa
    12841 tgcgacgaac gtcatacccg cggaggctcg ggcgaaattc aacatccgct acaacgattt
    12901 gcaccggcgg cccagcgtcg aagcctgggt gagggagcaa tgtgctgccg cggcggaaga
    12961 agtgggtgcc cgctggtcgg tgacgttttc gggcaccggc gacgtcttcc tcaccaagcc
    13021 cggaccactg gtcgacacca tggtggaggc ggtccgagcc gtaaccgggc gtgtgccacg
    13081 cctctccacc agcggcggaa cgtcggatgc gcgcttcatc caggcctact gcccggtcat
    13141 cgagttcggc ctcgtcaacc agacgatcca ccaagtcgac gaacacgtgc cgattgggga
    13201 tctcgagcag ctgacggcga tctacgagcg gttcatcgca ggctatattg cagcggcggt
    13261 gtagatggcc cgtagctgcg ccgttgcaag ttcatgaact ttgaactatt gaacaattga
    13321 tgtccatggg ccatactgac atcatgcagc ggccgttcgt tcacccagcc atcgacgaca
    13381 tagcgctgga gagcattctc tacgcgctgg ctgatcctgt gcggcttgcc atcatgctca
    13441 agttgacatc gggcgggtgc ccgatgaact gctcgacggc agcgccggag aagctgccga
    13501 agtcgacgca atcgcaccac tacaaggtgc ttcgcgaggc ggggctgatc cgctccgagc
    13561 ggcgcggcac cgaggtggtg aatacgctga gatgccacga gctcgacgcc aagttccctg
    13621 gggttgtcgc ctccattctg aaggccgccg cagcacagat gaatcgcgag gcgaagaagg
    13681 ggtgcgagcc cgagtccgtc tgacctcctc ccgccccccg cttgctactt gaaatgcttg
    13741 gagagcttca gcccctgtgc ctggtaatgg ctgcccaggg cggcgccgta gagcatctcc
    13801 ggcagctccg ccatgcgctc gtagatcagt cttcctatga tctggccatg ctccaggatg
    13861 aacggcacct tgtgcgagcg cacctcaagc acggcgcgtg agcccgcgcc gccggacggc
    13921 gcatagccaa atcccggatc gaagaagcct gcgtaatgga cccggaattc gccgacgagc
    13981 ggattgaacg gcaccatttc cgctgcatgt gtcggcggca cgttcaccgc ctcgcgtgag
    14041 gcgaggatgt agaactggtc gggatcgagg atcagccgct tctcgccccg gagcgtgatc
    14101 ggctcccagt aatcgagcac ggcgcacgag ccaggcgcat cgacatcgac gagtcccgtg
    14161 tgccgcttgg cgcggtatcc gacgagcccg cttgcgtccc ccgcaagatc gaccgacagc
    14221 gcgatcccat gagagatatc gggccgatcg gaggagacga ggcgctcgcg ccggtgcaga
    14281 tcggcaagag ccgcgtcgct gtccgcagcc gtaccctgcc ggaagcgcac ctggcacagc
    14341 cgtgatccct gccgcacgat gattgggaag gtctgcggac agatctctgc gtacaacggg
    14401 cctgcatatc ccgcaggtac ctgatcgaaa gccgacacgc cgtcggcaat gacgcgcgtg
    14461 aacacgtcca gtcggcccgt cgagctcttc ggattggccg ccgcggatat gccagagggc
    14521 agcgcaaggc tttccagcaa aggcgcgacg taaacgcagc ccgtctccaa cacggccgat
    14581 tgcgtcaagt cgatcttgtg gagggcaagt tcattgagct tgtccgaaat cgggatattc
    14641 ggcccaggca gaaagctcgc acggacccgc cacgcacagt cacccaacct caaatcgagg
    14701 cttgcaggct gcagctgatc ctgcagcatc ggctgcgcca ggcggacttg tccctccgcg
    14761 atcagacgcc ggatggcctg aaccggcaga atgccgttcg cttgcgtccc tgaacctgct
    14821 ccatcgctca tgccgtacct ccgccaggct ctgtctagcc ggtccagtga cgtgcgccaa
    14881 ggcgacgcga atcgtgcgca tcgaccgcgc aagcttgacc ttggttcgaa ccaggcgtat
    14941 cagcacgact ctcgatcgtg gtgatttgcc ggccggcttg cagccacgtt aaacaactcg
    15001 ctaaaaggcc tcgcgcaacc tggcctgcgc ccggcattga tgcgggaccg ccggccgggt
    15061 tttctttttg ccttggccag atgggtagga gggcgccaat ggcgcagcgc gacacagtat
    15121 cagaccgcgg ccccagtcag aactgggctc ccgagacact ccttgttcac ggtggacagc
    15181 tccgaaccgg cttcggtgag accgccgagg ccctgttcct gacccagagc tacgtgtacg
    15241 agacggcgga acaggccgag gcccgtttca agggcacgga gcctggcttc atttactcgc
    15301 gcttctccaa cccgacggta tcgatgttcg aggagcggat gcggctgctt gaaggtgcag
    15361 aggcggcccg ggcaaccgcc acgggaatgg ctgcagtgac atctgcactg ctgagctacc
    15421 tgagcgccgg cgaccacgtc atcgcggcgc ggtcgatgtt cggctcctgc cgctacgtgg
    15481 tcgaggagct ttgtccccga ttcggcatcg ctgctactct ggttgacggg ggaaatatcg
    15541 aggcttggcg caaggccatt cgtcccaaca cgaaagccgt gttcttcgaa accccggcaa
    15601 acccgacgct cgaagccgtt gatatcaaag cggtatccga tctggcgcac gacgtcggcg
    15661 cactgatctt cgtggacaac gtgtttgcca cgccgcttct gcagaagccg ctgacgctcg
    15721 gtgcagacgt ggtggtctat tcggccacca agcacatcga cgggcagggc cgttgcctcg
    15781 gcggcgtcgt gctgtgctcg gagaagttcc tcaaggacca ccttcatacc ttcctccgcc
    15841 agaccggccc gtccctcagc ccgttcaacg cctgggtctt gctcaaagga ctggagacgc
    15901 tgccgcttcg cgtgcacgcc cagtgcgcga cggccaccaa aatcgcggac tggctggcgg
    15961 aaaggcccga ggtcagccgc gtgctctact gcggccgtgc cgatcaccct caggctgagc
    16021 tgatccggaa gcagatgtcc ggaggcggac aggtggtgac gttcgagatc gctggcggga
    16081 agccggcggc gttcaggttc ctgaatgcgc tccgcctgat ccggatctcc aacaatctcg
    16141 gagacgccaa gagcctgatt acacatcccg caacgaccac gcatcagcgg atcaagcccg
    16201 aggtccgcgc ggagctgggc atcagcgacg gcatgctgcg cctgtcggtc gggctcgagt
    16261 cggcggatga tctgatcgcc gacctcgcgg cagggctgac tgcggcgcgc tgagtcagtc
    16321 cttcctatca cgctatcacg ccggcgtccg atccgagctg atcgggcgcc ttcgcttttt
    16381 tgcgtcgtag gcgcctcgac ctgccttcgc gctgctccgg gcggcgcgca ccgctgatat
    16441 gggctgctcc gtgggaaagg cggccagctc ctccagaaag cgccttctcc acgggggacg
    16501 acagttatcc ccagataaac tcactcttca tatttggcat gcaatcttca aagattgacg
    16561 gcatataaga gggattgtac gcagatatcg tagacgatca atctgattcg gggggcagca
    16621 atgatcgtaa tcgtcgacga gcgcgaaacc gtgtcggatg ggtttgcgtc ctggttcgac
    16681 cgggagggcg tatccgcgac aggcctctgc ccagtggatt tcgaccagtg ggtcgagacg
    16741 gtttcccagc gtgacatcat ggctgtcgag gcgttcctgc tcggcgattg ccgcgagcga
    16801 cactcactgt cgcggaagat aaaggggcga tccggagctg ctgtcatcgc gatgaacgag
    16861 aagcggtcgc tggaggaaac gctcgagctg ttcgcggcgg gcgtggatga tgtcgtgcgc
    16921 aagccgatcc atgtccgcga gctcctggcg cggatcgagg cgatccgccg gcgtgcgcag
    16981 cgggacgcag tggatgaggg caccgaggcg ggtgacctgc gcgtgttctt cgatgggcgc
    17041 gacccggagg tgaaagggga gccgctgccc ttgccacgcc gcgagcggcg catactggag
    17101 tatctcgtga tgaacaaggg acggcgcgtc accaagagcc agattttcaa ctcggtgtac
    17161 gggctttttg gagaaaacat cgacgaaacg gtcatcgaaa gccacatcag caaattgcgc
    17221 aaacgcttgc gccagcggct gggtcacgac ccgatcgatt cgcaacggta tctcgggtac
    17281 cggctcattt accaatagtt actggttagc gcagcggggt agcgccgcgc ggggagcggt
    17341 cgttgtgcgg agcggccgct cccaaatttc aaaaaaaggc tcgcgcaagt tcagcgtggt
    17401 ctggtgaagc gacgaaaaaa gcggggcaac tgaaatgagc ctctatggaa tgatgcgaac
    17461 cggcgcgtcg ggaatggcgg cacaatcgag tcggctcgga acggtggcgg acaatatcgc
    17521 caacatgagc acgacaggct acaagcgggc ttcgactgaa ttttcaacga tggttctcga
    17581 cccgggcgcg ggcagctacg tttctggcgg cgtcgaatcc cacacgcgat atgccatcag
    17641 ctctcaagga ccgttagcct acacgacgtc cccgaccgat cttgccgtgc gcggtgaagg
    17701 ctttttcctc gtatccggtc caggcggtca aatctacatg acgcgggccg gatcgttcgt
    17761 catgaatggc gacggccagc ttatcaatgc ggctggctat agcctcatgg gctatccgat
    17821 tgctggtggc gatccgaata tggtcgcgaa tggctttgcg gggctcgagg tcatcaacgt
    17881 ccgcgacctt gcaatgcagg caagcccctc gaccgaaggg aggttctacg caaatttccc
    17941 cgccggtgca gatatcatcc ccgccgccga tctgccgtcc gccaatgctg caacggctgc
    18001 cttcaccggg aagacctcac tcgtgacgta tgacaatctc ggcggcgagg tcatcatcga
    18061 tatctacagc tccaaggtgg ctggcgaaac ttgggaagtg acggtattcg atcgctctgc
    18121 cgcggctgca gggggaggtt tcccctatgc ctctggtcca ctggccagcg taacgctgac
    18181 ctttgatccc gcaactggca agctcgatgc cgccagcccc acttccatcg gcattccgat
    18241 accgaatgga gcgacgctga atttggatct ctcccagtcg agccagttgg cggcggatta
    18301 cgtcgtggcc gatccggtag tgaacggcaa cgcaccgagc gacgtcgagg ggatcgaaat
    18361 atcggaagct ggagaactgt ttgcggttta ccgaaatgga gcgcgtattg cgacgcaccg
    18421 cattccgctc gccaatgtga cgagcccaga caatcttcag ccgttggcag gcaacgtcta
    18481 taccgcaacc gccgaatccg gagatgtgcg gatcgggttt tcgggagctg gtggcttggg
    18541 gaggatcgtt tcgggagcgt tggaacaatc gaacgtcgat ctcgcatccg agctgacgac
    18601 gatgatcgag tcgcagcgca actataccgc caattccaaa gtcttccaga ccggcgcaga
    18661 tttgatggac gttctcgtta atctgaagcg gtgacatgcg gcttaatccc gtatcgctcg
    18721 agatgatcgc gatatgacac tcacttcggc gatagacgtt gcccgcagtg ggctctccgt
    18781 gacgtccggt caaacggccg ttgtctcgcg gaatatcgtc ggtgcgggcg aggcacttgc
    18841 ttcgcggaaa ttggccaacg tcgtaaccgc gccaggcggc ggcgttcgtt tggcatccat
    18901 ttctcgcgtc tccgatcgcg cgctttttgc gcacatgctt gcatcgaact ccgcggcggc
    18961 gctgcaacaa agcatcgtcg atgcgctgga ccggcttgat gggaccacac tcgacccgga
    19021 gcttgacgcg tcgccggccg ctttgattgg caagctgaac gacgcgatac agctctactc
    19081 tgctactccg cacgatgccg tcgcagccca gtctgcagtg gcagccgcgc gggatgtcgc
    19141 cctggctctg aatgatgcaa cggacacgat tcaggggctg cgccagcagg cagatgccga
    19201 aatggctagc gcagtcgatc gactgaacgt gctgctaacc cagttcgaga aactgaatac
    19261 tgcaatcgtc aatggcactc gcgcgggcag cgacgttacc gacgacctcg acaaccgtga
    19321 ccgcctgctg gaggcgattt ccgaggagat cggtattcga acggtgctcc gtgccgataa
    19381 cgacatgctc atcttcaccg atagcggggt cacgctattt gagaaaaaac cgcgcttgct
    19441 ctccttcgag cccacgctga tttatgcgcc gaatacaatc ggtaatgccg tttacgcgga
    19501 cggggtgcag atcacaggca cgcccggcgg catgagaatc agtaatggcc ggctggcagg
    19561 gctggctacg gtccgcgacg atatcaccgt gagatatcaa tcacagctcg atgaaatcgc
    19621 tcgcgggctc atcgaaacat tcgccgaaag cgaccagagt gcgcctccga ctctccccga
    19681 cgcgccgggg ctcttcacat ttccctccgc tacgagtgta cccgctcctg gggcgctggt
    19741 ggtgggtctt gcaggacaaa taaccgtcaa tccgagtgtc gatcccgccc aaggagggga
    19801 tgtcacgaag ctgcgggacg gtggcctggc tggtccggaa tacgactata atccgagcgg
    19861 ggcagccagt ttctcaggac ggcttcaaga aatgctggaa aacatggcga cgcaacggcc
    19921 attcgatccg agcgcggggc tagctgcatc agccacgatc gccggctttg ccgccgattc
    19981 tgttgcttgg ttgcaggaaa accgtatgtc ggccagcagc agtctcgatt accgacagac
    20041 ggtgctcgag cgttcctcgg aagcgctgtc caacgcgacg ggtgtcaatc ttgacgagga
    20101 aatgacgatc ctgctcggcc tggagcggtc ctaccaagca acctcgcggt tgatctcgat
    20161 aatcgacgac atgttcaggg cgctcctcgc ggcggcagga taagcagcaa tgataacatc
    20221 cgtatcgacc agatcgcttt tcggagcaac aagaacgaca ctcgtcagag ctcaaagtgc
    20281 gttggcgctc gcgcaaaagg agaccacgac cgcacgtcat gcggatgtcg ggaaaagtct
    20341 cggcttcatg accggccacg caatatcgtt ccgcaacgac tatgcgcaac ttggtgccat
    20401 acgtgatgcg aacagcattg cgcaaacccg gctgtcagca acgcaatccg tgctcgaagg
    20461 gttggctgaa gacgcgcaaa gttttctcgg ttctctggtt ggcgcgcgca acagcgacgg
    20521 tggcgcacgg gtcctcgccg tcgaggcgga ggcgcggctg aaatccttga tcaacggaat
    20581 caacactgca atagaaggcg cgcatatttt ctctggaatt gactccgaca acacgcctct
    20641 cgaagaatat ttcggcaacc ccacagcagc aagccgccaa gccgttgccg acgcgttcac
    20701 tggtgcgttc ggtttcgcgc cttccgatcc tgcggtaaac agcatttcgg aagccgacat
    20761 gcagggcttc ctctctggtg ccttcgcaga cctcttcgag gaaccgtcct ggtcggccta
    20821 ttggtcaagg gcatccgacg agaacatctc cagccgaatt tccaccagcg aagtcatcga
    20881 gacatctgcc aacgcgaacg cgagcgccat acggaagctc gtcagcgttt acgtcatggt
    20941 cgcggatctt ggcggggagc ggctcaatga tggcgctttt caggccatcg tcgacactgc
    21001 gatcgaggca accggagctg cgatccagga tctcacgggc ttgcggacga agctcggcat
    21061 cgcggaggac aggattgcga ccgcgaacga acggatctcg atcaaaatgg atgtgttgac
    21121 gaagcacatc actaaccttg aggctgtgga cccctacgaa gcatccctgc gcgtgacgtc
    21181 tctcctaatt cagatcgaga cggcttatgc gttgacggca aggctccagc agatgagcct
    21241 catttatcac ttataatctc aaacaagtcg tcttcagaaa atgagcaatc cttcggtcta
    21301 cgccgaaatc cttgaagaca cgcccaagct ggccagagag cgggagcggg cagcgatcgg
    21361 gcgggctgtc gatctgctgg aacgggcttc tgtgaaaggc ccccgctcgc gggaggcggt
    21421 ggaggcattg gattatctca atcggctctg gtcgattctc attgaagacc tggccaaccc
    21481 tgaaaacgat cttcctcaga ggttgcgagc cgatctcatc tccattggct tgtgggtgct
    21541 acgcgaagtg gatcgcatcc gcctccagca gtcaggaaat ttctccgcat tggtggacgt
    21601 gatgaacacc atccgggagg gcctccagtg agcggcagca ttcagatttc cctgcgcgcg
    21661 ggtgaaaggt tctatgtcaa cggggcagtc ctccgggcaa accgcaaggt aacgctcgag
    21721 ctgttgaacg aggcgacatt tctcctggaa caacacgtca tgcaggcaga gggggcgacc
    21781 acacctctgc ggcaattgta tttcgtcgtt cagaccatgc tgatcgatcc cgccaaagca
    21841 gaggaagcaa ggagtttgtt cgaaaaattt cataagctgc ttaagacgtc gttcgagagc
    21901 gcggcggtgc ttgcggggct cgaacgcgtt catcagctcg tcgccgcgga tcgagcgttc
    21961 gaggcactga agactctgcg tgtcctcttt ccgatcgagg acgacatctt ggcagcaggg
    22021 cagattcagg ctcatgccct accggcgcag acccacaagg cggaggcggt gtgatggagg
    22081 ttgcggcagc agcggggagc tcgccaactg gactcaccgg aggcactggc gccaagtcgc
    22141 gggcacagct cgattacgac gcctttctgc agctcctcat tgcgcagatg aagaaccagg
    22201 atccaaccca gccgatggaa tcgaccgaat acatcgcgca actcgccgca ttctccaatg
    22261 tagagcaagc ggtccagaca aatgcaaagc tcgattctct aatggcggcg ctgtcgctgt
    22321 cgcaggcgga cggtgtcatt ggtcgcacgg tgagctcggc cgatagtgac atcagcggga
    22381 aagtgacggc attgcgcgtt gctgagggcg gaaccgttct tattctcgac aacgggcagg
    22441 agttgccctt cggaccgggt atcacgatca cctgatcatg aaccaggcag atgcactgga
    22501 tatcgtccag acaacgatct ggaccgtgat cgtggcggca agcccggcaa ttctcgcggc
    22561 catgctggtc ggcgttgcga tcgcgctcct gcaagccctg acccaggttc aggaaatcac
    22621 cctgaccttc attccaaaaa tcattgcgat cctgctcacg gcatcggtat cggcgccgtt
    22681 catcggggcg actatttata cgtttgcaga gcaggtgtac ggccggatag agcacggctt
    22741 ttgaagccgc gtgccttggt tctcggggga ggggatggca gcgacagcag ccccgcgcgt
    22801 cacagcaacg agccgcggcg gtggtcacga catctggttc gcaatcggga tcgtgttcat
    22861 cctgactgtc ctgttcctgc ctttgccgcc ggcgctcata gacatcggac tggcattctc
    22921 ctttgcattg tcgatcctgg tcctcatggt ggctctttgg attgacaaac cgctcgaatt
    22981 ctcctccttt ccgacgatcc tgctgattac cacgatgctg cggctgtcgc tcaacatcgc
    23041 aacaacccgg ctcattcttt cgaacggcgc cgagggcgtc acggctgcag gtcacgtcat
    23101 cgccgggttt gcaagttttg tcatgagcgg cgatttcgtg atcggggcga ttgtcttcgt
    23161 gatccttgtc accgtgaatt tcgtcgtcat cacgaagggc gccactcgca tcgccgaggt
    23221 cggggcgcgt ttcactctcg atggtattcc cggcaaacaa atggcaattg atgccgacct
    23281 gtcggccggc attatcgacg acaaggaggc aacccgccgg cgccgggagc tcgaggaaga
    23341 gtcctcgttc ttcggcgcaa tggatggcgc atcgaagttc gtccgcggtg atgccgttgc
    23401 cggtctcatc atcacgggcg tcaatatcgt cggtgggatg gtcatcggta tagcgcgcca
    23461 tgatctttcg ccggcgcagg ctgccgatgt tttcaccaag ctttccgtcg gcgatggcct
    23521 cgtttctcag atcccggccc tcatcgtctc gctcgcggcg ggcctccttg tttcaaaggg
    23581 cggcacacgc ggctccgccg aaaaggcggt tctcgggcag ttgggccgct atccgcgcgc
    23641 gcttttcgtg gcgggtctcg tcgttgcggt cctggctatc gcgccgggtt tgccgtttct
    23701 gccgtttgca agtttggccg ctctcctgac ctcggttgcc ctggctattc cgcgccgtca
    23761 ggctgaggcc gcggccgaag cacaagcgcg tgaggcggtg gaacgtcaaa aagcggagga
    23821 cgaagctcgt tcatcgatca aagcgtcgtt gaaggcagcc gagatcgaag tctgcttggg
    23881 taaacagatt tccaccacgc tgatcgcggc acacggcgag ctggcgcaga gggttaccaa
    23941 gatgcgccgc aaatttgcgc gggagttcgg cttcgtggtt ccggacatca agctcagtga
    24001 cgacctcgcc ctgcccccca agacctatca gatcaaaatt catgggaccg cggtggcaaa
    24061 ggccgaactg cgcatcggcg atatgctcgt tattatcggt gatggcccgc gtcccgatgt
    24121 tcccggtgag gagtgccaag agccggcctt tgggttgaaa gcgctttggg ttcccgaggc
    24181 cctcgccgct aatgtcaagc gggatggatt gacaccgatc gatccgccga cagttctcct
    24241 gacgcattta cgggaggtca tccgcaataa cctggcgcag ctgctatcat ataaggacgt
    24301 gagagctctc gttgatgggc tcgaaccgga gtatcgtcgc ctggcggatg agcttatgcc
    24361 tgcgcagatt tcctactcaa ccttgcacgc ggtattgaag ctgcttctcc aggagcgcgt
    24421 ttcgatccga aatttgcatc tcatcctcga agcgattgcg gaggcggttc ctcacatccg
    24481 gcgggtggag cacctcgctg agcaggtgcg catccgactt gcccaacaga tctgcggcga
    24541 tctggccgag aatggcgtcc tcaagatgct tcggctcggg aaccgctggg atcttgcatt
    24601 ccatcaggcg ttgaagcgag acgcgaaggg ggaggtggta gagttcgata tcgagccgca
    24661 gctgatcgag cagttcggtg cagaggcggc gaaggcaatt cgctggcaca tggaccgagg
    24721 ccatcgtttc gccgttgttg cggcgcctga ggcgaggccc tacgtccgca tggtcatcga
    24781 gcggctctac cctgcgctgc ccgtcctctc gcatctcgag atcgcccgcg gcgtgcagat
    24841 cgaactgctg ggcacgatct cttgacaggc ccgctttccg acacagtgct gctcactttt
    24901 ctgttgttct gccgaattgg cgcttgcctg atgttgatgc cggggttctc cagtccgcgc
    24961 gttccggtgc aaattcgcct gttcgtggcg atcgcggtca cgctcgcgct cactccaatt
    25021 ctacttccgg ttctgaaatc ttcggtcttc cggccatcct tgagcgccgc gttcgttttg
    25081 ataatcgggg agacgcttac tgggggattc attgcgctcc tggtgcgcat ttacttcatg
    25141 gcccttcagt tcatggcggc agcgcttgcg aatttcgtcg ggttttacgg cttttccgag
    25201 atgggagtgg aggagtcgga gccgaatgcg ccgctggcag gtctgattac gctggcggct
    25261 gccgttctgt tctttatcgg cgacctgcat tgggaagtgt tgaagggtat cgtgcaatcc
    25321 tatgctgcgc tccctcttca gggtcccctg gttgtcgagt tcgggctcgc gcgcttggct
    25381 gaagcgacgg cggatgcatt tcttcttgcc ctacagatcg ccagcccgtt tctcgtttac
    25441 gcattagcgg tgaacttggc atttgggctc ctgaacaaac tgacgccgca gatccctgtc
    25501 tatttcgtct cgcttccctt cgtgatagcc ggcggactgc tcttgcttta tttcgttgcc
    25561 ggagagtttc tcagcttctt tatggttgct gtcgagagtc ggctgatacg gggatagccg
    25621 cacaagccag gggtaaggta atcgggccaa gttggaggga gacccaggaa ccttttctgc
    25681 tccggctatg ccgcttttca ctccaccaag cttcgtggcg caaggcagag atgtgggcta
    25741 cgtaccgccg tctccgccgc aaatgtcgca acgcccgggt gtaaacccag ctgcggatac
    25801 cgctgtccgg gagtttgaag ggttcgtcct gcagatgttc cttgaagcga tgttgccgga
    25861 agaagcatcg agcgtcttcg gaagcggcac gacgggggcg ttctggaaat caatgctggc
    25921 agagcagata gccaagcagc tcgcaggcgc gggcgggatc ggtgtcggtc ggttgatcgc
    25981 cgatggcata gccgccactt cgagagacgt gggctgatgt cgtccgtcgg ggtgttcgtt
    26041 gatcgtgtca tcgatcacct tgaacaggtg atccaggaag aaacgtcgct gctgaaaagc
    26101 cggcaagctg tcgatctata cgagttcaac aaccgaaaaa gccagggcct ctacaacctc
    26161 ggccgcgcat tgttgatgct ggagggacgc aagctggagt cgtctacact ggcgaagctc
    26221 gagtctctac gtcgcgcctt aaccgcaaac gaagcagcga tatcggttca cctccatgct
    26281 gttcgggaga tcaccgacgt gatttccaag gctgttcggg agtccgaatc ggacggcacc
    26341 tacgaagcgc ccgacccact gcggggcaag gtatgatcaa gcttctcgcc ctgggcgtct
    26401 ggatcacagc tgtgacgatc gcatcgagct ttgccgccct gtcttggaaa actgggcagc
    26461 tgccgaggcc cggacccctc ggtctgttcg aggagctgac aacgatcaac acgcggctga
    26521 tcagcatccc attcattgcg gacggcgccg tgcagggata cgtcgtcacg aaactggcat
    26581 ttgccgtaga cactgcactt ctcaagcagt tctccgtcga accagagctc gttctggtcg
    26641 acgaggcgat acggaccatc tattccagta gcgacttcga ttttcggcaa atggccaggc
    26701 aggacctttc tcggttggcc aagacgctgg caaagaacgc aaacgaacgc ttcaaggcaa
    26761 acctcgtggc ggaggtgttc attcaagagc tgaattacgt caccaaggac atggtgagga
    26821 acggcgggaa tcggtagagt taaaatcaag aaggcccgcc gagtgctcgt agtgcctcgc
    26881 gcaacgaaga atctgtgtcg ttcaaggcac tcgtaatggc ctcgaaagcg cgcgttaccg
    26941 agacaaggcg agccatctct tcgatcggat ttacgttcgc gcgctcaacg taaccctgga
    27001 ctacaccagt ctgaacgaag tcgaggaccg gctctgcggg cctgtccgga agcacccctg
    27061 aattctcata gcgggcaagc gaggacagct ctgagatccg aaacaggccg atcgccccca
    27121 tttgttgccc gtcctgggta atggtaccat ccctggcaat ctgcggaggg ccggcgtttg
    27181 gattgagaac gatgggtgcc ccacccacgt ccagaacggc ataaccgttg agcgtctgca
    27241 actcgcccgc ttctgtcata cgcatgcggc cgtcccgggt gtagacctgc ccagcaggtg
    27301 tcgcgatggc aaaccatgcg tcgccttcga ttgcaacgtc gagaggattg tctgtcctgg
    27361 tgagctctcc gggccggcgg gagagatacg acttcccggc agacgagaat gcaacgctat
    27421 ccagcggggt ctgcgagagg atgctctcga atttgacctg ctcaccccga aatccagccg
    27481 tcgacgagtt ggccacgttt tgagcgatcg tgtcgagccg gcgttgtatg gcgagctgcc
    27541 cagaaagggc gacgtacaat ccggactgca tggtcagctc cccttgacct gtaggttttg
    27601 cagactggcg agcaacgagg gggtgatacc aacaccggct cgccagcccg ccgaaacagc
    27661 aggtgagttt gcctgcgtgc ctgggttggt gagttcccag aggctgagaa atcgggcgat
    27721 aaaccgctgt aacttattcg ggtccttgaa ctcctcgatg tcgaggcgcc gtgaaatcat
    27781 ctccgcctgc aggtcgatgt cggccagggc ggttgcaggg gggagtccaa gtgcagtctg
    27841 aacaacggcc agcagggcgc gatccgccag gatcccgtaa gcactcttca gcaaaggtgc
    27901 cttgcgctga aaatagagcg ccaggcgaac accttcgttt cggctgccgg catcctcttc
    27961 cagcatctgg cgaacatatt tatcaacagt gccctgtcgc gtgcgctcga aactggttgt
    28021 tgcggtgcca tatcgggcaa aattgaacgt gcttacgaaa tcgcgatacc gctggtcaga
    28081 aagctgattc gcaaagctct gtgggtcgtc gataccctcg gtcaaggcct ttcggatgaa
    28141 cgccttggcg taggtcatct ctcccagtcc gaatgccttc attgcatagg tgaagaggcg
    28201 attatcggcg aggaaatcat cgatcgattt tacctcgccg atcctttcaa ggtaatagtc
    28261 ggtttcccgt gcaacctgcg gtttccgggc tatggcggcg aggctgcgtg gcagatcgct
    28321 tgtgatcagc cgataggatg taaacgtcgt caacacgagc acgccctgcg gccaggaaat
    28381 agtgcccaag agtagcctgc gaggcttgtg tgaacccttt cgcccggccg gcttgttgtt
    28441 tggagcaatg ggggcgagaa caggctcaca caagcaccgt ggcctaaacg ggcgtgagag
    28501 gatcagaccg ggggtaccat tagcgtgacc atcatcatcg ggcttttgat tacgctcggc
    28561 gccatgctcg gtggcttcac ggcgatgggc ggccatgtcg aggtcatctg gcagccgtgg
    28621 gagtacgtga tcatatgcgg ggcggcgctc ggtacgttcg tggtcgccaa tccgatgaaa
    28681 accatcaagg atgtcggacg aggattgatc gaagtcatcg gaatgcgcac gccaaagccg
    28741 cgtgagtatc tcgagacgct cggggccttg catgacatca tgcgggagct acgcggccct
    28801 tctcgcaacg agctcgaggc gcagatcgaa aatcctcacg aatcaaagct cttccgaaaa
    28861 ttccccacag ttctggcgaa ccgtcagttg acgacgttca tttgcgatta ctgccgcctg
    28921 attctgatgg gaaatgcccg tacgcacgaa atcggggcgc tcatggacga ggagatcgag
    28981 acgatggtcc atgacagcct gaagccctat tacgcccttg tcaccattgc agaagcgctg
    29041 ccggcgctcg gaattgtcgc cgcagtcctt ggcgtcatca aggccatggg tgccctcgat
    29101 caggatccgg aaattctggg ctccttgatc ggggctgcgc ttgttggaac attcgccggc
    29161 atctttttct cctacgcggt cgtatcaccg gttgcgagca agctgcgcgc gatccgcgaa
    29221 aagaaaatgc ggctttacgt cgtcgtgaag cagacgctcc ttgctttcat gaacggtgcc
    29281 atgccgcagg tggcgctcga gcacggacgc aaggcaattt ctgcgttcga gcgcccatca
    29341 atcgatgaag tggagcagca aaccgtctca gggggcgaag gcgggccccg gagggaggcg
    29401 gcctgagtga gcaagacggc aacaaatgag ggggcgcctg ggcggcggaa tgcgttgctg
    29461 gagattttct gccgtggcgc tggctattcc gaacggctgc cgatgctgcc ggcggcactc
    29521 gagcgggctg cgcttgcgtg tacggatgag tttggatgcc tgtcgtcagt gcggccagag
    29581 atgagctatc tcggcctgga aagtgggcgc gccgcgcatc tcatcgagcg ggagaaggat
    29641 gcgcacctcg tcggcatgct gcatgcccct gcctgggacg cgcacttgct cgtcagcttc
    29701 gaccgcgatc tgatctacac ttacgtggag cttgttttcg gcggcgacgg cacgcagccg
    29761 ccatatgcag agccgcgccc actatctcgc atcgaaatgc gcatcgcaca aagcctactc
    29821 ggccgtttgg ccgggacgct gtcgcgcgct tttcatcctt tcgcgccgac cagcttcgcg
    29881 ccagaaggtg aactgggacg tatcgacctt gatcgcctgg gcggaccgaa tacgccggtt
    29941 gcgattgcgc gtttcaaact tcgggcgcgg caggcgaccg gcgatttgct gctcgcaatt
    30001 cctgatgcgg tgctcacggc gctgaagcct gccttcgcgc gtcgcgagcc accacgttcc
    30061 ggaacggccg atccggattg ggcgcagcag atccacagcg agatcaatcg ctcccggctc
    30121 acgatgcgag ccgtgctcga ggagcgccaa aggccgctct ccgaaatcgc cgggcttcgc
    30181 gttggtcaga tactgccgct cgatgccacg ccgcgcacgc ccatccgcgt cgactgcaat
    30241 ggcgaaccaa tgatgtggtg tgaattggga caacagaacg gaaattacac attgcgcgtc
    30301 cacgcttttg tcgatcgcga acaggagttc atggatgaca tcctttttgg gtgatgtttc
    30361 ccagcggcgc gctgcggcta ccgcgcaaag ccagccgaat gtacacgacc gggatatgag
    30421 agcaggtgaa agcggggcgc ggcctgactc cgctgcggtc gggcatgagt cgctaattca
    30481 ggaattcgct gccgagctca cgccatcgcc ggaggaactt gccgaagcct tcggaacgga
    30541 cgggaggagc catgatggcg ccttgccgaa acaggcgaga caggatcaaa gctccggcaa
    30601 taatatcgac atggtcatgc gcattcctgt ttcggtccaa gtggtcctgg gggcggcaag
    30661 gatgtccgtt tcgggactcg tcaagctcgg acggggtgcc gtcattcctc tggaccgccg
    30721 cgtcggggaa ccggtcgatg tcatggtcaa cgggcggctc attgcgcgcg gagaggtggt
    30781 cgtcatggac gagggtagct cccgcttcgg catttcgctc accgaaatcg tgacgggccc
    30841 gggatctgcc agggacgcct gacagatccc gcgcaatgac aaaagcacag gcgccagcca
    30901 ggccggagcc gaaatcgctc agcggcgtgg agaaagtgac cgtgctgctg ctggcgctag
    30961 gccgtcccaa ggcggcgcag ctcctgaagc gcatggatgc cgaggagatc aggctgatcg
    31021 cgcgggcagc gaatcagctt ccgttgatat ctgccgatga tctcgccgaa ttggtcgacg
    31081 aatttgcccg catgttctcc accggcgtca acttcgtcgg tacggcaaac gcggtcaagg
    31141 acctgctggc cgaggtgatg acggaaggag aagtcgcaga tcttctgtcc gataccgggg
    31201 gcgaggagga gcccatatgg agcagaattg cgcggctcaa ggacgaaatc atcagggcct
    31261 acctgctgcg ggagcatcca cagacgatgg ccctcattct atcgcggctc agtccgccac
    31321 gtgccgcgcg gctcatggga tcgcttccgg cggagttacg caatagcctg ctcatccgaa
    31381 tgctcggaat aaaattcgtc gcgccggatc agctcgaaat tctggaaaat acgctgcgtg
    31441 aggacctgat ctcgcttcag gccccctcgg gcggtacgca cgccggcatt gcggacattc
    31501 tgaaccggct cgacaaagcg cagttcgact tggccctgaa gcatcttacc aaggcgcgtc
    31561 ccgctgatgt cgaagctatg cgcgacatgc tgttcacctt cgacgacctc gcgtcattgc
    31621 cacccgggac actgggcgta atcctgaatc aagttccgcc tgaccgtctt gtgcttgccc
    31681 tgcgcgaaac ggactttgcg ttccaggacg ccgtactttc tgggctgccg gcccgtgcac
    31741 ggcgcatagt ggagctggag ttgcagacgg aaaataatga atccgctcgg gagattgccg
    31801 acgcacgacg cgccatcgtt gaaaccgttc tgaagctcgt cgccgagcgc cgcatcgagc
    31861 tgccggccac aaaagtccgc gcggcagcaa gctgagggag ggacgaggct tgtcggaagc
    31921 tcccgaccgg gaaagcagga cggaagaagc gagcgagaag aagatccggg atgccgttga
    31981 gcaaggcagc gttccgcatt cgcgcgaggt ggtgacattc gcatcgttgc tcgggatcct
    32041 gttgatcctg cgaatgtcct ttccgggcat tggacgtctg cgcgcggttc tggagcggtt
    32101 catcgacgat cccggcggct ggtcgcttga aaacggggcc gatgcagtgc acctgctcgg
    32161 gatcgttgga agcgcgatgg cgaaggagct gatcccgctc gtggccacat tgtgtgcctt
    32221 tggtctcggt gcttctctga tccagaacac gcctcggctg gtcgccgacc gcatccgccc
    32281 gcgcctttcg cgcctctcct tgtcccaagg gtggaagcgg atttttggcg cccagggcag
    32341 ggtcgaattc ctcaaggcga gtttcaagct cgccgcagtc ggctgcacgg ctgttttcct
    32401 gttgcgtgcc actcaggcgg acgtgttcaa cgccatgctg gcggagccga gtgcgctgcc
    32461 agagctcctg cgcgatgttt ccgttcgact ggtcacgggg atcaccgtag ccactctgct
    32521 gctcgcggtg gccgacctca tctggtcccg cgtgttttgg cgccgcgaat tgcgcatgac
    32581 acggcaggag atcaaagacg agctgaagca gactgatggc gatccgatca tccggtcacg
    32641 gctgcggtca ctcgcgcgcg accgctcccg ccggcgtatg atggccgccg tgccccgcgc
    32701 cacgctcgtc atcgcaaatc cgacgcacta tgccgttgcg ctgcgttatg tccgggagga
    32761 gggcggcgcg ccgcgcgtgc ttgccaaggg ccgggacttg attgcgctcc gcattcgcga
    32821 aatcgccgag cagcacaata tccccgtcat cgaagacaaa ccgttggcac gcgcgcttca
    32881 tgacaaggtc ggcatcgacc aaatgatccc gccggagttc tacaaggccg tagcacagat
    32941 cgtgtacttc gtgatgacgc ggaaaggtgg ccacgcacgg ggcggattgg ggctaacgaa
    33001 atagaacaag tctatcgttc tgattgaggg gaagcgacgc tcaccctttt gcctctcaat
    33061 gaggagaggc cgatcacttg ggcatgacgg agagtagcgg ctccaactcc ctattcgcca
    33121 ttcgctactc tctattcgct attcgctatt cgctattcgc cctcatatcc ccaacttctg
    33181 cttcagcagc tcgttcaccg ctgccggatt ggctttgccg ccggtagcct tcatcacctg
    33241 gccgacgaac cagccgagca tggtgggttt ggctttgacc tgctcaacct tgtccggatt
    33301 ggcagcaagc accttgtcga tggcttgctc gatcgcgcct aagtccgtga cctgcttgag
    33361 gccacgcttc tcgacgatgt cggccggatc gccgccttcg gtccagacga tgtcgaaaac
    33421 gtccttggca atcttaccgg agatcgtctt cgaggcgatc agatcgagaa tgccgcccag
    33481 ttggtccgct gaaaccggcg agtgctcgat atcgagacct tccttgttga ggcgcccgaa
    33541 cagctcgttg atgacccagt tcgctgccgc ctttgcatcg cgcccggagg cgaccttctc
    33601 gaagtagtcg gcggatgcgc gctcggccac gagcacacca gcgtcgtaag aggaaaggcc
    33661 gaactgctcc ataaaccggc gcttcttttc gtcgggaagc tcgggcagat ccgctttgag
    33721 cgcgtcgacc cattcctgcg tcaattccag cggcagcagg tcggggtcgg ggaagtagcg
    33781 gtagtcgtgt gcctcttcct tggtgcgcat ggagcgcgtc tcgcccttgt tcgcgtcaaa
    33841 caggcgcgtc tcctgaacga tctcgccccc atcctccaga atatcgatct gccggcgggc
    33901 ttccgcctcg atcgcctggc cgatgaaacg gatcgagttc acgttcttga tctcacagcg
    33961 cgtgccgaat ggctctccgg gcctgcgcac ggatacgttg atgtcggcgc gcagattgcc
    34021 cttctccatg tcgccgtcgc aggtgccgag atagcgtagg atcgtgcgca gcttgctgac
    34081 gtaggcctgt gcctgccggg ccgagcgcat atcgggcttg gacacgatct ccatcagcgc
    34141 gacgccggag cgattgagat cgacgtacgt gtaatccgga tgctggtcgt ggatggactt
    34201 gccggcatcc tgctcgaggt gcagtcgctc gatgccgacc gttatcgttt cgccatccgc
    34261 atcgatctcc acctcgccct cgccgacgat aggctgcttg tactgggaga tctgatagcc
    34321 ctgcggcaga tccggataga aataattctt ccggtcgaaa acgcttttca gattgatctg
    34381 cgctttgagc ccgagccccg tgcgcacggc ctgggcgatg cactcctcgt tgatcacggg
    34441 cagcatgcca ggcatggccg catcgaccag tgacacgtgg ctgttcggct ctccgccgaa
    34501 ttccgtcgag gccccggaga acagcttggc gcgcgtgttc acctgcgcgt gcacctccat
    34561 gccgatcacg acctcccaat ccccggttgc cccggcgatc agattgctcg atttccctgc
    34621 cttcgctcga tcctgcatgg ttcgctctgt tagctgcctg tcgcaaaggg cgcagaggct
    34681 ctaacccctc accccgaccc tctccctgag ggagagggag atttgttgcg gcaccgaatt
    34741 ctcaactcat ttccctctcc ccgaggggga gagggttagg gtgagggggc tccgcggagt
    34801 tgtagcgtgc gggctaacac gtcgagaact ccatcgacgt tctcatatac atccacattc
    34861 cgaaaccgga ggaccagata tccgaaagat tccagagcac aagttcgttc agtatctctc
    34921 attacttcat cttgcgtgct gtgcgttgcc ccatccactt cgactattac ctttgcatcg
    34981 aagcagacga agtccattac aaagcggtca atcggcactt gccgccggaa cttgaagccg
    35041 agaagctgcc ggtttcgcag gtgttgccag agcttctctt ctgctctcgt ctgcgagcgc
    35101 ctgaggcgcg cgccaaaacg atcgagccag tcacccttcc ttcgccttcg cctccagctc
    35161 gttgcgcagc ttctcctcct cgtactggat gaaccagccg atcatctgcc gccaaagcgc
    35221 ctcgcagagg tccggcggaa cgccgcgttc ggcggcgcgg gctcggacgc gatcgatgac
    35281 ctgctgattg cgccagggga cgttggcctg ctgcttggca gccagcttga gctgccaggc
    35341 ccgctccacg tagccgaagc gctccgcgat caagtcgacg agcgcggcgt cgagccggtc
    35401 tatctcagcg cgcacctggc tcatgtcggt gcaatcttcc ggtcgacgcg tgctcatcgt
    35461 cctccccctg tcaacctttc agccaccatc gatcgggcac ggtgaagcgc cccgcggcat
    35521 cctcgatgac ctggcccgcg cgaaacagcg tgccctcgtc gaacggcttg ccgatcagct
    35581 gcagtccgag cggcgttcct tggcgcgaca gaccacccgg caccgaaatg cccggcagcc
    35641 ccgccatgtt caccgtaacc gtgaagatgt cctcgagata catggcgatc ggatcgcccg
    35701 acttttcgcc gagggcgaag gccggtgacg gcgtggtagg cgtcaggacg acgtcgacct
    35761 tgtcccaggc ctcgtcgaag tcgcgcttga tgagcgtgcg gaccttctgc gccttgagat
    35821 agtaggcgtc atagtagccg gccgagagca cataggtgcc gatcagaatg cgacggcgca
    35881 cctccttgcc gaagccttcg gcacgcgtcc gctcgtaggt gtcgatgagg tcgtcgccgg
    35941 tgattctgag tccgtagcgc acgccgtcgt agcgcgcgag gttcgacgat gcctccgcgg
    36001 gagcaaggat gtagtaggtc ggcagggcgt acttcgtgtg cggaaggctg atctcgtgga
    36061 tcgaggcgcc ggcatcacgg agccacgcaa tgcccttttc ccacagctcc tcgatctcgg
    36121 cgcccatgcc ttccatgcga tattccttgg gaaccccgac cctgaggccc ttgatgctct
    36181 tgccgacctc ggcctcataa tccggcacgg gggcatcgac ggaggtcgag tccttcgggt
    36241 cggtgctcgc catgaacttc agcatgatcg ctgcatcgcg cacggtcttg gcaatcggac
    36301 cagcctgatc gagcgaggag gcgaaggcga cgatgcccca acgggagcag cgcccgtaag
    36361 tcggctttat gccgaccgtt ccagtgagcg ccgccggctg ccgaatggag ccgcccgtat
    36421 ccgtggcggt ggcggcgaga cacaggtcag ccgcaacggc cgcagccgat ccgccggacg
    36481 aaccgccggg aaccagcttt gcattggagc cgttgttgcg ccgccacggg ctgatgacgg
    36541 ggccgtagta gctcgtctcg ttggacgagc ccatggcgaa ctcgtccatg ttgagcttgc
    36601 cgagcatgac cgcgccggca tcccagaggt tctgggtgac ggtcgactcg tatgtcggct
    36661 cgaagccatc gagaatgtga ctgcaggcct gcgagtgcac gccacgcgtg cagaacaggt
    36721 ccttgacgcc gagcggaagc ccttcgagcg ggcgagcctc gccgcgggca atacgcgcgt
    36781 cgctgtcggc tgcttgagcc aacgcgcgat ccgccgtggt ggcgacatat gcattgagcg
    36841 caggattcgc ggcttcaatc gcatcgatga aagcctgggc cagctccttt gcggtgaagc
    36901 ttttcgcctt caagccctcg cgcgcctcgg cgagggtgag cctggtgagg tctgtcacgg
    36961 tcgaatgctc gtcgctttcg atacttcaat ccatagtgct attccctctc ggcgcctgcg
    37021 gcatgagagg gctgcggtgg gaggcgggac ccgcggcctg tgtctatcgg cgcctcacac
    37081 ctcgatccta tccccgcatg cgggaagggg gacttgagct actccaccac cttcggaacg
    37141 acgaagaagt gatcgtcggt catgggggcg ttcttgacga tgtcgtcggc gatattgcca
    37201 tccgtcacct cgtccttgcg cttcttgagg ctgatgggca cgacacgagt catcggctcg
    37261 acattgctgg tgtcgatctc gttcaactgc tcgacccaat tgagaatgcc cgtgagctcg
    37321 ctctggagcc cttgggcctc ctcatcggtg atcttgatgc gtgcgagcct cgcgatgcgc
    37381 cggacggtcg cctcgtcgac ctccatggtc cctcgctcat tccggaattg aaaccaacgc
    37441 cgcctctctt agcccgcgca aggcggcccg cgcaacagat tcacccgtga gaggccctca
    37501 gctggcgtca actcgattgc cctcagggca gagcaccggt gtcgatgctg gaccgggtga
    37561 cctgcgtgct gccaatgggc gtgctttcga tgaacacgtc gacctgctgg ccaggatagg
    37621 cgtgctttgt tcccgcaggc agggcgtaga tgacccgcag gacgcgcgta tcgacacgct
    37681 cgccggaggc gccggtcagc gagcgcttgg gaacaagcag cggctcgata ttcacgaacg
    37741 acagcctcat gcgctcggag gcgagccccc gcaggctggc ataggccggg gcggaaggct
    37801 caaagcgcgg cacatcagcc tcgtcgatgt cgatgcggac gtgcagcgga tcgagattgc
    37861 cgagcacgat cagcggtgtc gccagcgtgc cggcgaacgc gaactcgccc ggccggacat
    37921 tgacctgcag gaccgtgccg ttgatggggg cgcgcaccgt cagcagatcg aggtcgatct
    37981 cggctctttc caccgcacgg cgggcctgct cgacagccgc cgtggcaacg gccagcgatg
    38041 cgccgtccgg cggcgtgatg agcttcagct ccgcctcgat ctgcgcaatg cgggcttggg
    38101 cctcctcgag ccggccttcg gcagaacgct ccgcgttccg gcggtgcgtc acctcgcggt
    38161 cggaaatggc attgcctcca cgggccttga gcgcctcggc gacgcggacc tggtcggccc
    38221 actcctccat ctcggctcgc gccgcattgg ctgccgcgat cgccgccgcg cgctgtgcct
    38281 tcagggtcgc ctccgtcgcg agtatctgat cccgctgacg ttcggcggct gccagctccg
    38341 cccggcgcaa tgcgaggctg cctcgggctg cccgttcatc cagcacgaac agaggatcgc
    38401 cggccttcac cttctgaccg ggcttgacca gaacctcgga aacgatcccg gaagtgtgag
    38461 acccgatctc gatctcctgg ccgttggact cgatcagacc ggcggccccg atgaagcccc
    38521 gtgcggggcc gccagccgcg cctgctgcgg tgcttggctg aaccggcggt gagatggccg
    38581 gcaccagcgt cacctccacc ggctggctgc gggtaatgga caccgctgcc cccgcggctg
    38641 cagcgagcgc gacgagaggc agtccgatga tgagaagcct ggagcgtctg gccattcagt
    38701 ggccctccgt gaatggggga agctcgatcc ccgtgatgcg cccgtccgac atgcgggcga
    38761 tgcgatcggc gaagtgaaaa atccggttgt cgtgcgtgac gacgatcacg gcgcggtcgg
    38821 ggcgcaacgc gtgctggcgc agcagttcca tggcctgctg accggcctga gcgtcgagcg
    38881 acgccgtcgg ctcgtccgaa atgacgagcc gcggctcgtg caccagtgcg cgggcgatgg
    38941 cgacacgctg ctgctggccg cccgacagct cggccggaaa tttgtccatg tgctgctcga
    39001 gcccgacggc ggcgaggagc tcgcgcgcct tgcgcaaggc ccgagactcg gatgcaccgt
    39061 tgacgagcaa tggcactgag gcgttctcgg ctgccgtcag ggccggcagg agattgaatt
    39121 gttggaagat gaagccgatc gtctccgccc ggaactgggc cagtcgggcc gcgctgaggg
    39181 ctcccaagcg cgtcccgaac agctcgacct cgccctcctc ggcggacaga atgccagcga
    39241 tgaccgagat cagcgtagtc ttcccgcagc cggaggggcc gacgatgtag gtcatcgagc
    39301 cggtctcgat atcgagatcg atgccatgca ggacccggat gcgcgtctcg cccgagccaa
    39361 actctttcac aatgcccctg cagcggacgg ccaggttagg cgcagtgctg gcctgggtga
    39421 tctcgcgtgc aagcttcagc atcggctcac ccccggaaca cgatggcagg gtcgataacc
    39481 agcacccgcc ggaggctgac gatcgccgag agcagcatga ttgccgtcgc aagcaacccg
    39541 accccgaccg ccacctgcca gggcagatag aagccacgga agaacgacgt cgggttcgac
    39601 gtcaccgcct cgaagaagac cgacgtcaag ccgaggccga tcgcatatcc gatagcgcct
    39661 acgatggtgg cctgcagcag caccattccg atgaggcggc cattggtgag gcccatggcc
    39721 ttgagggccg cgtactgctt caggttctcg atcacgaaca tgtggaatgt cagcccgacg
    39781 acggcgatgc cgacaatgac gccgagcagc gtgacggtgc cgaagttcac gggaatgccg
    39841 gtgtggcgca ggaaatacat catcgtctcc caccgaaacg catcggaggt caccgccttg
    39901 agcccggtac gctcctcgat cgcttgggcg acggcggctg ggtcggcatc cggcgcggag
    39961 cgggcgagga cgaaggagag ctggttgcgg ccgttgttgg tgaagaccag cgcctgcgag
    40021 tagcgggtgt agatcagcgg ctgtgtctgg aacggtggag ccgtccgcag gatggcgacg
    40081 accacggcgc ggcggtcgtt gagctcgagg gtttggccga gggtcagcgg ctgggtcggc
    40141 cacaacttct tgtatcccgc ctcgtcgatg gcgatggcat cgggccgcct tagcgactcg
    40201 cgatcaccga ggatgatctc gggcggcagc ccgatcagtg tgacgtcgtc gaggccatag
    40261 acgaatgagg actgcaggcc gcccgtttcg gtggtcacga ccgcctggcc cttgaagaat
    40321 ggcaccgccc aggccacgcc cggcacgccg cgcacgcggc cgagctcagt atcgcgcatc
    40381 ggcctgacgg catcgatctg ctctatggcg gggtccatga cccagatatt cggctcacgg
    40441 atctccgtga tgagattggc cgtgcgctgc atgacgccga tgaacatgct cgcctgttgg
    40501 ctgatgagca gggtggagaa ggccatgccg aaaacgaggc cgagcagctt ggccgcgtcc
    40561 ccgaatagca tcttcagcgc gacccggttc atgtccgctc cctcacgccg tggtgtcggg
    40621 cggcgcattg cccgtcttca gaaactgcag cagcagctcg acgtgggttt cgagaattct
    40681 ttcggcgtcg agtggccggt ggcgttccat gagcagcttc cagaccatag ccatcagcat
    40741 cggtgcgatg acgagctgcg ggaactcccg cagcggcgtc tcgcggaatt cgccgcgggc
    40801 gatgccacga tcgatgatcg tccgaatgac gctcatgccg cggctcacca cttcctcgta
    40861 gtagaacgca gtgagatccg gatgcctttg accttcggca atcaggagcc gcaccagctc
    40921 gtgcacccgg gagttgacga tctttttcat caacccgagc acgggtcccc tgagcagggc
    40981 ctcggacgag agctgggatc cttcgatctc accctgcagc atctcgacat ggggaacgag
    41041 gaaagtgcgg atgaccgctt cgaataggtc ttccttggtg tcgaagtaga cgtagacgag
    41101 ccccttggaa atgccggcgc gccttgccac gtcgtccagt cgcgtgccgg cgtagccctt
    41161 ggcgatgaac tcttcgaggg ccgcgttcag gatctcttcc gggcgttgtt ctttgcggcg
    41221 cttgcgagtg ctcatgggtg cctccaacgc gaatgacata gctatgactg accggtcagt
    41281 caatataagg aaggctggcc gccgatgatc cgtggccgac gaaatttgcc ccgcagtcgc
    41341 ggcctggctg tgctttcacg ggcactgtca gagacttagg ccgcgatcag ccacgcgttc
    41401 gccatgatgc tgccacatgc agcgacaatt caacgcagga tgatcgacgc gctgcttccg
    41461 actgctctcg ctggccggca cgcccggcgc gccgccggtc ctctgatcgt ggcttgcttt
    41521 tctgctgttt tcctgattgc gcttccgtcc gggtcggcgg acgcggccga tcgcgtcaac
    41581 gccatgatcg aacggctcga cgccagcgag cggcggattt tcctggagtg gcgcgcggcg
    41641 caaaagaagc acgaagccgc gctcgacgcc tactggggga aggtgcagtc gacgcgaagc
    41701 cagcggcgaa agaagatcag ggcgggccgg cccgtcagtg ccgatgacta cgtcatgacc
    41761 ttcccgccgg agtattccgg gccgaagttg ccgtcgcata tcgcgcgcat ctgggacgag
    41821 ctgaagccac cggtggagcg taagcctgtg ccgagcgtcg cggacgtgct cgaaagcgcc
    41881 aaggctgtct acggcttcgt accggagcgc atcccagaaa aggagttcaa gcggcgatac
    41941 gcgctggagg cgctggcgaa cgggctctcc agggagcagg tcgttcgtgt ctacgcgctc
    42001 gagacgggcg gccgcgggac gtatgacatg caggccggga tcaatccttc gaccgggcga
    42061 gggacgccga tttcaaccgc gctcggctat gcgcagctct tgcacgccaa ttcggtcaac
    42121 gagctggtga agcacgggcc gacgttcatt gcccgcctcg agaagacggc tgagactccc
    42181 gggatagcgc cggagcgggc tgcagccctg cagaagaagg cgcgcgtgct gcggcggatg
    42241 ctggcgagcg ccaagaaggt gcccaacgac tggtacgcgc acatgcggtt tgcggccacg
    42301 cgcaacgggc tcggaattca tgcgctgaat ctcgacggcg acatcggccc catgctgcag
    42361 gtgatcaagc tgaaaggcgt ccgccaagtg gccgagcggg ctgggcgaac cgatctcgcg
    42421 ccggccgagc tggaactgat gaacctcgcc ggaccgagca cgggcctcga aatgatgacg
    42481 cccatcggcc ggaccgcacc gacgccgaat ttcttctccc ggctcggcta tgagcgaaat
    42541 cccgtcgtgc acaagcgtac cgcggccgag ctgctcgaga agctcgagtc cataatggac
    42601 gcgaacgtga agaagccagg ctccatcgag ttcgcagccg ccttcgacga agccatgtcg
    42661 atccgctcct cgcagcgata agcatccacc cctcgtgccc gagaagggcg ggtctcagca
    42721 gcttgacttt tgccgggccg ctgaggcgca cgcaacgctc gtctggctcg caagagggag
    42781 cggcccgccc tatattcgtg tggcgctccc atgcttgacc attccccctc agccgcgatg
    42841 tccgcgggag ctcgaatgtt aattcgattt ttgcgcgcac caattaagat cagccctatt
    42901 ggccggatct gacccgcgga ccggcgtgga acggggaggc ggaaccgatg tcgcagttcg
    42961 tgttcgccgt tattgccatc gcgctcgtat tcctgctcgc catgcgccgg gcgagcttgc
    43021 ccgtttgggc tggcgtggcc acggccatga ccttcgcatg gcagatcggg ctggccgggg
    43081 gaggtcccag cgccttcggg ttctggagcc tggtcgcctg gcttccggcc ctcgcactcg
    43141 ccgtgctctc tgtcccggag gtgcgccgca gccttgtcgt cgagccggca taccgcgcga
    43201 tccgcagctc cttgccgaag atctccgaga ccgagcagca ggccctggag gcgggcacga
    43261 tcgggttcga tgccgagctt ttctccgggc gccctgactg gaaccgtctg cgcgcagttc
    43321 agccgatcgt cctgacggcc gaggagcagg ccttcctcga tggacccacc gaggagctct
    43381 gccggatgat cgacgactgg cggatccgca cccgcgagcg cgagatcccc gaggaaatct
    43441 ggtccttcgt gaaggagaag ggcttcctcg ggatgctaat ctcgaagcag cacggcggcc
    43501 tcgggttctc ggcgcaggcg cagtcgctca tcctgggcaa aatttcttcg cgctcgccgg
    43561 acgtggccac catcgtcatg gtgccgaact cgctgggccc cggcgagctg atcgaaaagt
    43621 acggcacgcc ggagcagaag agccattacc tcccgcggct tgcgcgtggt gaggaaattc
    43681 cgtgctttgc gctgacgggg ccgacctctg gctcggatgc cgcgagcatg cgcgacgtcg
    43741 gctacgtaac gcgcggtgtc cacgaggggc gggagacgct cggtatccgc gtcatctggg
    43801 agaagcggta catcacgctc gcccccaagg cgacgctgct ggggctcgcg ttccgcctct
    43861 tcgatccgga gaatctgctg ggcaagggcg aggacgtcgg cattacgctc gccatcattc
    43921 ctaccagcca taccggcgta aacattggcc ggcgccattt gccttgcggc gcggcattcc
    43981 ccaacggccc gacctggggc aaggacgtct tcatccccat tgattggatc atcggtgggc
    44041 aggccagggc cggccagggc tggcgcatgc tgatggaatg cctgtcggca ggtcgctcca
    44101 tctcgctgcc atcctcgtcg gcggccggca tcaagacgat gctccgcttc tcgacggctt
    44161 acggccgaat ccgccggcaa ttcggcctgc caattgccag aatggaaggc gttgaggagc
    44221 cgctggcgcg gctggtcgag aacgcctacc tcgttgaggc gggccgtgcg gtgacagcgg
    44281 caatggtcac tcgtggcgag aagccggcag tgctcggcgc gctgatgaag tatcagtcca
    44341 cggagcggat gcgccaggcc gtcaacgacg ccatggatgt gcacggcggc cgcggcatct
    44401 gcgacgggcc aacgaactat cttcagtctg cctatcaaat ggtgccgatc gcgatcaccg
    44461 tcgaaggcgc caacattctc acgcggacgc tcatcacctt cgcccagggc gcactgcggt
    44521 cgcatccgta tctctatcgc gagctgcagg cggtccagaa ccccgatccc aaacgggggc
    44581 ttgagctttt tgagcccatt tttctcgacc acgtgtcgtt cagcg
//
LOCUS       DASVDU010000322         8129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1063134, whole genome shotgun sequence.
ACCESSION   DASVDU010000322 DASVDU010000000
VERSION     DASVDU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063134"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VF226_21660"
     CDS             <1..1436
                     /locus_tag="VF226_21660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017991782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX7076656.1"
                     /translation="NALHQAASQAVSIETSNDIYNEAARRAIADLYMLMTHMPEGLVP
                     YAGIPWYSTVFGRDSLITALLMLWFDPGVALGVLRYLAANQATDVIPEADAEPGKILH
                     EVRLGEMAELGEVPFRRYYGSVDSTPLFVFLAGSYLERTDDLETIRNLWPHIEAALNW
                     IDQFGDQDGDGFVEYGAKTKKGLVNQGWKDSYDAIFHANGDLAKGPIALVEVQGYVFA
                     AKKKAAVMAERLGHEALAAKLLREAEELQKRFQKEFWSDEIGTYALALDGKKRPCLVQ
                     TSNAGHALLTGIAADECVPKVVQALMSRESFSGWGIRTVATTASRYNPMSYHNGSVWP
                     HDNALIAMGFARYGFKEAASRIFEALFTASSYLELRRLPELFCGFGRRRSHGPTLYPV
                     ACAPQAWAAAAPLALLQSCLGLRISPKQGTISLEHPMLPPFLDHVTLRKLACGGIKLD
                     IEFRRAGDHVAMNVLNATGDAQLKMTV"
     gene            complement(1702..2832)
                     /locus_tag="VF226_21665"
     CDS             complement(1702..2832)
                     /locus_tag="VF226_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7076657.1"
                     /translation="MNPKNLPFNSEEMLAGLKPWIECESPTYDAAAVDRMMDLAAYDL
                     AAAGAEIERIPGRMGFGGSVRARFPHKNFGSPGILISGHLDTVHPIGTLKVNPWRIEN
                     GRCYGPGIQDMKGGNYVSLEAIRQLLRAGLETPLPVTFLLTPDEEVGTPSTRELIETE
                     GRRNKYVLVPEPALADGGAVTGRYAIARFNVKTFGRPSHAGFLLKEGRSAIAAMARKI
                     LEIEAMTTEDCTFSVGVVHAGQWVNCVSSTCEAEVLSMAKRQEDLDRGVARMLELSGE
                     ENDVRIEVTRGVTRPVWEPDAGTMALYGIAREIAREIGFELTHASAGGGSDGNFTGAL
                     GIPTLDSIGVRGSGLHTLNEHIEIASLVERAQLIAGLLMRLT"
     gene            complement(2859..4019)
                     /locus_tag="VF226_21670"
     CDS             complement(2859..4019)
                     /locus_tag="VF226_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX7076658.1"
                     /translation="MPALPRIADYADELTAIRRDFHQHPEIGFEEVRTSGIVADLLKS
                     WGVDEVHTGIGKTGVVGIINGKSEGRSIGLRADMDALPMDEQTNLPYASKSPGRFHGC
                     GHDGHTTMLLGAARYLAETRNFAGRVVLIFQPAEEGLGGARAMLADGLFEKFPCDEIY
                     GFHNNPFAEPKKVGVKPGTAMAGASFFDITVQGVGSHGAMPHHARDPIIAATSLVQNL
                     QTIVSRNVEPTRPAVLSVTQIHSGAAYNVVPDKAVISGTIRFFEDPVKELIHERVRQL
                     CSGFAQSYGLEITVDLRDIFEVLVNDEACSKAYLEAAAEIVGEENVSETKELMTGSED
                     FADMLKAIPGAYCTIGHKGSVPLHNPAFVFDDDVLPIGASILARIVERRSPL"
     gene            complement(4053..5714)
                     /locus_tag="VF226_21675"
     CDS             complement(4053..5714)
                     /locus_tag="VF226_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076659.1"
                     /translation="MKLKQRNFDGAPVVLDVRDLTVRLSGNREVPPVVDQVSLQIRSG
                     ETACLVGESGSGKSVTALSIMGLLPKGALEVAGGEIILGGTDLLKLGPQAMRQMRANR
                     ISMVFQEPMTALNPVMRVGEQIEEVLDTHARLSAGEKKSRVLDILNQVHLPDVERIYR
                     SYPHELSGGQRQRIMIGMALILKPQLLIADEPTTALDVTTQQQILNLIAELQEHHGTA
                     VLFITHDMGVVAEIADTVHVMKHGEIVEHAPVAKLLREPRDDYTRRLLRSVPSLVPRP
                     PRPANGEAKVLEVAGLEKIYISRSLFSRREPVRAAVDVKFSIGRGRTLGIVGESGSGK
                     STVARCIMRLIDPTSGEIRVAGQEFAKLPRRALRPYRKHIQIVFQDPFRSLNPRWKIG
                     ESLIEGPLNCGQDRKSALARAAELLQLVGLPADALDRYPHQFSGGQRQRIAIARAIAM
                     EPDVLVADEAVSALDVSVQAQVLDLLDELQQKLGIAILFITHDLRVAAQICDDVVVMQ
                     HGRIVESGSAAEVLARPQHPYTRALIEAAPGRFWDFANFRELAAT"
     gene            complement(5719..6573)
                     /locus_tag="VF226_21680"
     CDS             complement(5719..6573)
                     /locus_tag="VF226_21680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076660.1"
                     /translation="MQWMLGAIALPRSWRAGVGPVIAAIVLLVIVLSAIFAPLIAPHD
                     PLSMNAVQRLKGPSDLYPLGTDAYGRDLFSRIVTGGRISLFIGIGAALVSVFIGLLIG
                     LVSGFFRLADAIIMRIMDSLMAIPSILLAIALVSLNGPSIWSVMIAITIPEIPRVVRL
                     VRSVVLSAREEPYVEAAIALGTPMPKILWRHLMPNTLAPLTVQGTYICASAILTEAIL
                     SFLGAGVSTEIPTWGNIMAEGRVYFQLKPSLIFWPGLMLSLCILSINLLGDTARDVLD
                     PRFKKREV"
     gene            complement(6603..7544)
                     /locus_tag="VF226_21685"
     CDS             complement(6603..7544)
                     /locus_tag="VF226_21685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7076661.1"
                     /translation="MLGYVVRRILAVIPVMVSVAIFVFLLLRLTPGDPAAIIAGDMAT
                     PAQLERIRQSLGLNEPIHIQFITWIKLLLQGDLGTSLISNTPVTRMIGHRIWPTINIA
                     LLTITLSVLIAVPMGVLAAWRHRTWADYAVMTFSVLGFSIPVFVIGYIFIQIFSIELR
                     WLPVQGYTAPSEDFGRFLSRAILPCLTLATIYVALIARMTRASMLEVLGEDFIRTARA
                     KGVKERIVLFRHALRNAAVPIVTVIGTGFALLIGGVVVTESVFNIPGVGRLTVDAILA
                     RDYPVIQGMILLTSGLYVLINLLIDLSYTFFDPRIRY"
     gene            complement(7657..>8129)
                     /locus_tag="VF226_21690"
     CDS             complement(7657..>8129)
                     /locus_tag="VF226_21690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076662.1"
                     /translation="APTDVTTLTAQPVVASQALREAGFNVDMQPMDWQTLVTRRASQA
                     KPSEGGWNLFFTNWMVPEINSPLISPMLNGRGDQGWFGWPKDDKLEELRAEFIAADTP
                     EKQREVAIKIQKHTLENVIYIPLGQYAAPQARSKKLTGMIPSPVPVFWNVDKAD"
BASE COUNT         1614 a         2424 c         2590 g         1501 t
ORIGIN      
        1 ggaatgctct ccatcaggcc gcctcgcaag ccgtaagcat cgagacgtcc aacgacatct
       61 acaacgaagc cgcccggcgc gccattgcag atctctacat gctgatgacg catatgccgg
      121 aggggcttgt cccctatgcc ggcataccct ggtacagcac cgtcttcggg cgggattcgc
      181 tcatcacggc gctgctcatg ctttggttcg accctggagt cgcgttgggc gtcttgcggt
      241 atctcgcggc caatcaggca accgatgtca ttcctgaggc cgatgccgag ccggggaaga
      301 tcctgcacga agtcaggctc ggcgagatgg ctgagctcgg tgaagtgccg ttccggcgct
      361 attacggttc cgtcgattca acgccgctgt tcgttttcct ggcgggctca tacctcgagc
      421 gaacggacga cctggaaacc attcgcaatc tctggcctca tatcgaggcg gcgctgaatt
      481 ggatcgacca gttcggcgat caggacggag acggcttcgt tgaatacggg gcaaaaacaa
      541 agaagggcct cgtgaatcag gggtggaaag acagttacga cgccatattc catgcaaatg
      601 gggatctcgc gaaaggcccg atcgcgctcg tcgaggtgca gggctatgtt ttcgcggcga
      661 agaagaaggc ggcggtcatg gccgaacgcc tcggccatga ggctctcgca gccaaactct
      721 tgcgtgaagc ggaagaactt cagaagcgct ttcagaagga gttctggtcg gatgagattg
      781 gcacctacgc gctggctctc gatggaaaga agcggccctg tctcgttcag acctcgaacg
      841 ccgggcatgc gctgttgacc gggatcgcgg cggatgaatg cgtgcccaag gtcgttcagg
      901 cgctgatgtc gcgagagtcg ttttcaggct ggggtattcg gaccgtcgcg accaccgcgt
      961 cgcgctacaa tccaatgtcc tatcataacg ggtccgtctg gccgcacgac aatgcactga
     1021 ttgcgatggg cttcgcgcgc tatggcttca aggaagcagc gagcagaata ttcgaggctt
     1081 tgttcaccgc ttcgagctat ctggaattga ggcggttacc ggagcttttc tgcggttttg
     1141 gtcgacgccg cagtcacggg cccaccctct atccggtcgc atgtgccccg caggcttggg
     1201 cggcagcggc acccctcgcc cttttacaat cgtgcctggg cctcaggatc tcgccaaaac
     1261 agggcacgat cagtctggag catcccatgc tcccgccatt cctcgaccac gtgaccctac
     1321 gaaagctcgc ctgcgggggt atcaaactcg acatcgaatt ccgtcgcgcc ggcgaccatg
     1381 tggcgatgaa cgtgctcaac gccacaggcg atgcccagct gaaaatgacg gtttaaattt
     1441 tttccaatat agctggaaac gagccaagcc aagctcacgc gctctatctc tctttacaat
     1501 ggggagaggg tcggattgaa gggggcagtg accactgttg caacccctca tcccgccctt
     1561 cgggccgacc ccggatcgag tccggggcag gctctctccc cacgggagag gtgaagggag
     1621 taaccgctgc ttgcgtgccg ctaccaccca gttcgaataa tcgatggcgc gttttcagac
     1681 cccgctcgaa agaagcggtg atcacgtcag tcgcatcagc aggccagcaa tcagttgggc
     1741 gcgctcgacg aggctggcga tctctatgtg ttcgtttaat gtgtgcaggc cggagccgcg
     1801 cacgccgatg gaatcgagag tcggaatgcc gagggcgccc gtgaagttgc cgtcggagcc
     1861 gccgcccgcg ctcgcgtgcg tgagctcgaa cccgatttcc cgcgcaatct cgcgcgcaat
     1921 tccgtaaagc gccatggtcc ctgcatccgg ttcccagacg ggtcgcgtga cgccacgagt
     1981 cacctcgatt ctgacgtcgt tttcttcgcc tgaaagctcc agcatgcggg cgacaccgcg
     2041 gtccagatcc tcctggcgct tggccatgct gagcacctcg gcctcacagg tcgaagatac
     2101 gcagttgacc cattgcccag cgtgcacgac accgacgctg aatgtgcagt cctcggtcgt
     2161 catggcctcg atctcgagga tcttgcgggc catcgcagcg attgccgatc ggccttcttt
     2221 gaggagaaaa ccggcgtggc tcgggcggcc gaaagttttc acgttgaaac gcgcaatggc
     2281 gtaacggccc gtgaccgctc cgccgtcggc gagtgccggc tccgggacga gcacgtattt
     2341 gttcctgcgc ccctcggttt cgatcaactc gcgggtcgag ggtgtgccga cctcttcatc
     2401 gggagtgagg aggaacgtta caggcagcgg cgtctcgagt ccggcgcgca acaattgccg
     2461 gatggcctcg aggcttacgt aattgccgcc cttcatgtcc tgaatgccgg ggccgtagca
     2521 gcggccgttc tcgatccgcc acggattgac cttgagcgtg ccgatcgggt gcaccgtatc
     2581 caagtgtcct gaaatcagga tgccggggct gccgaagttc ttgtgtggaa agcgagcccg
     2641 caccgagccg ccaaatccca tccggcccgg aatgcgctcg atctcggcgc ctgccgcagc
     2701 aaggtcataa gcggcgaggt ccatcatgcg gtcgaccgcc gctgcatcgt aggtggggct
     2761 ttcgcattcg atccagggct tcaatccggc cagcatctct tccgaattga agggcaaatt
     2821 tttcgggttc atggccattt cctccaatct gaaaagcgtc aaagcgggga gcgccgctcg
     2881 acaatgcgcg ccaggatgct tgcaccgatg ggcaacacat catcgtcgaa aacgaaggca
     2941 gggttgtgca gcggcaccga gcccttgtgg ccgatcgtgc agtaggcgcc ggggatcgcc
     3001 ttgagcatgt cggcgaaatc ctcgctgccc gtcatcagct ccttggtttc cgagacattc
     3061 tcttcgccta cgatctccgc tgcggcttcg agataagcct tcgagcacgc ttcgtcgttg
     3121 accaggacct cgaagatgtc gcgcaggtcg acggtgatct ccagcccgta gctctgcgcg
     3181 aaaccggagc agagctggcg gacgcgctcg tgaatgagct ctttcaccgg atcctcgaaa
     3241 aagcggatcg ttcccgagat cacggccttg tcgggaacca cattgtaggc cgcgccggaa
     3301 tggatctgtg tcacggagag caccgccggg cgcgtcggct ctacatttcg gctcacgatc
     3361 gtctgcagat tctggaccag cgaggtcgcc gcgatgatgg gatctcgcgc gtgatgcggc
     3421 atggcgccat ggctgccgac gccctgaacg gtaatgtcga aaaagctcgc gccggccatg
     3481 gcagtgccgg gcttcacgcc gactttcttc ggctcggcga agggattgtt gtgaaatccg
     3541 taaatctcgt cacaggggaa tttctcgaaa agaccgtcgg ccagcatggc gcgggcgccg
     3601 cccaaaccct cctcggcggg ctggaagatc agaacgacac ggccggcgaa attgcgcgtt
     3661 tcggcgaggt agcgtgccgc gccaagcagc atggtcgtat ggccgtcgtg tccgcagcca
     3721 tgaaaccggc caggcgattt ggatgcgtag ggcaaattcg tttgctcatc catcggaagg
     3781 gcatccatgt cggcacgcag gccgatcgag cggccctccg atttgccatt gatgatacca
     3841 accacgccgg tcttgccgat gccggtatgg acctcgtcga caccccaaga cttgagcaag
     3901 tcggcgacga tgcccgacgt gcgaacctcc tcgaaaccga tttccggatg ctggtggaaa
     3961 tcgcgccgaa tcgcggtgag ttcatcggcg tagtcggcga tcctgggcag ggcaggcata
     4021 gcgatggtgc tcccttgatg tttggccgcc gatcaggtgg cggccagttc ccggaagttg
     4081 gcaaaatccc agaagcggcc cggtgccgct tcgatgaggg cgcgcgtgta cgggtgctgt
     4141 ggcctggcaa gaacttcggc cgccgatccg gattccacaa tgcgcccgtg ctgcatgacc
     4201 acgacgtcat cgcagatctg ggcagcgacg cgcaggtcat gtgtaatgaa aagaattgcg
     4261 atgccgagct tctgctggag ttcatcgagc aaatcgagca cctgcgcctg gaccgagacg
     4321 tcgagtgccg agaccgcttc gtcggccaca agcacgtcgg gctccatggc aatggcccgg
     4381 gcgatcgcaa tgcgctggcg ctgcccgcct gagaactggt gcggatagcg gtcgagagca
     4441 tcggccggca gcccgacgag ctgcaacagt tcggcggcgc gggcgagagc cgatttgcgg
     4501 tcctggccgc aattgagggg gccctcgatg aggctttctc caatcttcca ccgcggattg
     4561 agcgaacgga acggatcctg gaaaacgatt tggatgtgct tgcgataggg acgcagggcc
     4621 cttcgcggga gcttggcgaa ctcctgacct gcgacgcgga tctcgcccga cgtcggatcg
     4681 atcagacgca tgatgcagcg ggcgacggtg gacttgcccg agcccgactc cccgacgatt
     4741 ccaagcgtgc ggccgcggcc gattgaaaac ttcacgtcga ccgccgctcg aaccggctca
     4801 cggcgggaaa agagggatcg agaaatatag attttctcga ggccggcgac ctccagcacc
     4861 tttgcctcac cgttggcggg ccgcggtggg cggggcacga ggctcggaac cgatcgcagc
     4921 agacgcctcg tgtaatcgtc ccgaggttcg cgcagaagct tggcaacggg tgcgtgctcg
     4981 acgatttcgc catgcttcat cacgtgcacc gtgtcggcaa tttcggcgac gacgcccatg
     5041 tcatgcgtga tgaagagcac ggccgtgccg tggtgttcct ggagctcggc gatgaggttc
     5101 aaaatctgct gctgcgtggt gacgtcgagc gcggtcgtcg gctcgtctgc gatgagaagc
     5161 tgcggtttga ggatcagcgc catgccgatc atgatgcgct ggcgctgacc gccggaaagc
     5221 tcgtgcgggt aggagcgata gatgcgctcg acatcaggaa gatgcacctg gttgagaata
     5281 tcgagcacac ggcttttctt ttcaccggcc gaaagccggg catgcgtgtc gagcacttcc
     5341 tcgatctgct cgccaacgcg catgaccggg ttaagcgcgg tcatcggctc ctggaaaacc
     5401 atcgagatgc ggttggcgcg catctggcgc atggcctgcg ggcccagttt caagaggtct
     5461 gttccgccga gaatgatctc gccgccggca acttcgaggg cgcccttcgg cagaagcccc
     5521 atgatcgata acgccgtgac ggacttgccc gaaccgcttt cgccgacaag gcaagccgtc
     5581 tcgccactgc ggatctgcag cgagacttgg tcgacaacag gcgggacctc gcgattgccc
     5641 gaaagccgga ccgtgagatc gcgaacgtcg agcaccaccg gcgcgccgtc gaagtttcgc
     5701 tgcttgagct tcattccgtc agacctcccg cttcttgaac cgcggatcga gcacgtcgcg
     5761 cgcggtatcg cccaggaggt ttatggacag aatgcataac gacagcatca ggccgggcca
     5821 gaaaatgagc gacggtttga gctgaaaata gacccgcccc tctgccatga tattccccca
     5881 ggtcgggatt tcggtgctca cgcccgcacc gaggaacgac agaatggctt ccgtcagaat
     5941 ggccgaggcg caaatatagg tgccctgcac cgtcagtggc gccagcgtat tgggcatgag
     6001 gtggcgccag aggatcttcg gcatcggcgt gccgagggcg atggctgctt cgacgtaagg
     6061 ctcctcgcga gccgagagca cgacggaccg cacgaggcgg acgacgcgcg ggatctccgg
     6121 aatggtgatt gcaatcatca cggaccagat gctcggtcca ttgagcgaga ccagcgcaat
     6181 ggcgagtagg atcgacggga tggccatcag gctatccatg atccgcatga tgatggcgtc
     6241 ggccaaacgg aaaaatccgg agacgaggcc aatgaggagg ccgatgaaaa cgctgacgag
     6301 cgctgctcca atgccgatga aaagcgaaat ccggccgcct gtgacaatgc gggaaaaaag
     6361 atcacgcccg taggcatccg ttccgagcgg gtagagatcg ctcggccctt tgagccgctg
     6421 gaccgcgttc atgctcaacg ggtcatgcgg cgcgatgagt ggcgcgaaga tcgccgacag
     6481 cacgatgacc agcaagacga tggcagcgat caccggaccc acgccggccc gccagctccg
     6541 cggcagggcg atggcgccca gcatccactg catgcgggaa atcggttgcg gtgcagcggt
     6601 catcaatagc ggatcctcgg atcgaaaaag gtataggaga ggtcgatgag gagattgatg
     6661 agcacgtaaa ggccgctggt cagaaggatc atgccttgga tgaccggata atcgcgcgcc
     6721 agaatggcgt cgaccgtgag ccgtccaacc ccggggatat tgaagacgct ttccgttacg
     6781 accacgccgc cgatcagcaa agcgaagcca gtcccgatca cggtcacgat cggcacggcg
     6841 gcattgcgga gcgcgtgccg gaaaaggacg atgcgctcct tgacgccttt agcgcgtgcc
     6901 gtccggatga aatcttcccc cagcacctcg agcatgctcg cccgggtcat gcgggcgatc
     6961 agggcgacgt agatcgtggc cagggtcagg caaggcagga tggcacgcga gagaaagcgt
     7021 ccgaagtcct cggagggggc tgtataaccc tgaacgggaa gccagcggag ctcgatggaa
     7081 aagatctgaa tgaaaatata gccgatgacg aaaacaggaa tggagaaccc gaggaccgaa
     7141 aacgtcatca cggcatagtc ggcccaggtc cggtgccgcc aggctgccag cacgcccatc
     7201 ggcacggcga tgagcacgga gagggtgatc gtcagcagcg cgatgttgat cgtcggccag
     7261 atgcggtggc cgatcatgcg cgtgacaggg gtgttggaga tcagcgatgt gccgagatca
     7321 ccctgaagca ggagcttgat ccaggttatg aactggatgt ggatcggctc attgagcccg
     7381 agcgactggc ggattcgttc gagctgggcc ggcgtcgcca tatcgcccgc gataatcgca
     7441 gcgggatcgc cgggggtcag ccgcaaaaga agaaaaacaa agattgcgac gctcaccatg
     7501 accgggatga cggccaggat tcgtcgaaca acgtagccca gcatcgggcg cagtctcctt
     7561 ggcgagccgc tgctgttgcg aacggcccgc gcggcggctc gaaccgccgc gcggaaatgc
     7621 gatcgggaac gcgctgccca cgtctccgca ggcgggtcag tcggccttgt cgacgttcca
     7681 gaaaacgggc accggtgaag ggatcatgcc cgtcaatttc ttgctccgtg cctgcggggc
     7741 agcatattgg ccgaggggga tgtaaatcac gttctcgagc gtgtgcttct gaattttaat
     7801 ggccacctcg cgctgcttct caggcgtgtc cgcggcgatg aactccgcac gcagctcctc
     7861 gagcttgtcg tccttcggcc agccgaacca gccctggtcg ccacggccgt tcagcatggg
     7921 gctgatgagg ggcgagttga tctccggcac catccagttc gtgaagaaca ggttccagcc
     7981 gccttcggag ggcttcgcct ggctggcgcg acgcgtgacc agcgtctgcc agtccatcgg
     8041 ctgcatgtcg acgttgaagc ctgcttcacg cagcgcctgc gaggcaacga caggctgcgc
     8101 cgtcaaggtg gtcacgtccg tcggcgcca
//
LOCUS       DASVDU010000323         6331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1256527, whole genome shotgun sequence.
ACCESSION   DASVDU010000323 DASVDU010000000
VERSION     DASVDU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256527"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1336)
                     /locus_tag="VF226_21695"
     CDS             complement(14..1336)
                     /locus_tag="VF226_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076663.1"
                     /translation="MARKSWGLAAAAAFAGLALAMPAQAQNEQFLPALVYRTGPYAPN
                     GIPFANGVADYWTLVNERDGGINGVKIVFEECETGYATDKGVECYERLKGKGPTGAGY
                     FSPLSTGITFALTEKAPGDKIPLLTGGYGRSDSRDGSVFTWNFIALGTYWTAADVAIQ
                     HIAKELGGMDKLKGKKIALVYHDSPYGKEPIPLLQKRSELNGFQLLLIPVTHPGVEQK
                     SQWLQVRQNRPDYVLLWGWGVMNSTAIKEAAAVAYPRDKIIGGWWSGAEPDVTPAGAQ
                     AAGYKALMLQHGSGKFPVHAEIEKYVYGKKLGAGELGDIGHVLYNRGLINSMIGVEAI
                     RTAQEKFGKKPLTGEQIRWGFEHLNLTAERIKELGFEGMIRPLKFSCEDHEGARVARL
                     HQWDGKAWHVISDWYEGDDSLLAPLVKETAAAYAKEKGITPRDCSKVE"
     gene            complement(1457..2545)
                     /locus_tag="VF226_21700"
     CDS             complement(1457..2545)
                     /locus_tag="VF226_21700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076664.1"
                     /translation="MLYREAGQFKTTYAADLAIFPIRQDRIALGVLLAFAFVGVPLLA
                     EFEIGAFGHDYIFKAILIPFLILSLAAIGLNILTGYCGQISLGGGAFMAIGAYAAYNF
                     ALRIPELPLLVSILLGGAMSALVGIFFGIPSLRIKGLYLAVATLAAQFFFDWLFLRVQ
                     WFTNYTPSGSISAPTLDFFGWVVNTPAERYMLALLFVTCFAIIAKNLTRGNIGRQWMA
                     IRDMDIAAELMGIRPLYAKLSAFAISSFIIGVAGALWAFVHLGSWEPLTFNIDRSFQL
                     LFMVIIGGLGSILGNFLGAAFILILPIFLDQVPHALGIPLSVETVSHLVFIITGALIC
                     FLLIVEPHGFARLWSIAKEKLRLWPFPY"
     gene            complement(2561..3490)
                     /locus_tag="VF226_21705"
     CDS             complement(2561..3490)
                     /locus_tag="VF226_21705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076665.1"
                     /translation="MEGLGPFFEVLVGGLLAGVLYSLVALGFVLIYKASGVFNFAQGA
                     MVLLAGLALVRSLDLLVANGMPFEVAVVGALLFAGAIMAITAWLIERYVLGPLLNQNE
                     LTLFMSTIGATFVIEGAAEMLFGSDVYPLALYPTDAWILLDEVFPGGILISKLDVWGG
                     VIAGILVAGLAAFFQWTKTGRALRAVADDHAAAQSVGIPISWIWFVVWLVAGLVALVA
                     ATVWGTKLGVQYSITFLALKALPVLIIGGFTSIPGAIVGGLIIGAGEKLAEIYLGPLI
                     GGGIEYWFAYVLALAVLIVRPQGLFGERIIERI"
     gene            complement(3625..4449)
                     /locus_tag="VF226_21710"
     CDS             complement(3625..4449)
                     /locus_tag="VF226_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076666.1"
                     /translation="MNAEAAVLDRPTADPKPAEREVLLAVENASLSFGGVHAIRDVSF
                     DIRKGEIRAIIGPNGAGKTSMLNIINGFYHPQRGTITFKGKRRHRMRPSIAARQGIAR
                     TFQNVALFKGMSTLDNIMTGRTLKMRTGILGQAVYWGWSQKEEIRHREFVERIIDFLE
                     IEHIRKTPVGKLPYGLQKRVELGRALATEPELLLLDEPMAGMNLEEKQDMSRFILDVN
                     DQLGTTIALIEHDMGVVMDLSDRVVVLDYGVKIADGTPDEVQNDEKVIDAYLGVAR"
     gene            complement(4446..>6331)
                     /locus_tag="VF226_21715"
     CDS             complement(4446..>6331)
                     /locus_tag="VF226_21715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7076667.1"
                     /translation="DLGIWQTWTWSQVCDEVRAFAIGLYVLGLKRGETVAIIGDNRPQ
                     LYWTFAAAQSIGAIPVPVYQDAVAAEMVYVLSHAEIAFAVVENQEQVDKILSLADQIP
                     TLRRIVYEDPRGLKKYDHTRLHAFEEVQRLGREELKANPGAGDWWLDEINQGKGSDTS
                     VILYTSGTTGQPKGVVLSFENLIRTARNANAFDKLSEKEEIVAYLPMAWVGDHIFSYG
                     QAYEAGFCVSCPESIETTVVDRREIGPTFFFGPPRRFEAALTDIFVRMQDAGRLKRWM
                     FDRFMDVARRCGEPILEGKPVPLSDRILYWIGDKLVYGPLRNQLGLSRLKIGYTAGEA
                     IGPEIFKFYRSLGINLKQLYGQTEATVYVTMQPDGEIYADTVGPPAPEVDIKIDDNGE
                     VLFRSPGVFQLYYKNEEATAAVKTPDGWVRTGDAGFFDSRGHLVIIDRAKDVGRLKDG
                     SLFAPKYLENKLKFFPEVREAVAFGHGFDFVTMFVNIDLVSVGSWAERNNVVYASYQE
                     LAAHPDVYAMIERRVDEVNAMLAREPKMASSQIRRFLVLPKELDADDGELTRTQKVRR
                     GLIAERYAPLIRALYDGSKQAHIRTEVTFEDGRKGIIEADVRIVDMPEHQPEVQALKE
                     AAE"
BASE COUNT         1288 a         1996 c         1862 g         1185 t
ORIGIN      
        1 tcgctattcg ctcctactca accttcgagc aatcacgcgg ggtgatcccc ttttccttgg
       61 cgtaagccgc tgcggtctcc ttgaccagcg gagccaggag agagtcgtca ccctcgtacc
      121 agtcagagat cacgtgccag gccttgccgt cccactggtg gaggcgcgcg acgcgggcgc
      181 cttcgtgatc ctcacatgag aacttcaaag gccggatcat gccttcgaag ccgagctcct
      241 tgatgcgctc tgccgtcaag ttcaggtgct cgaagcccca ccggatctgc tcgcccgtca
      301 gtggcttctt gccgaacttc tcctgcgccg tgcggatcgc ctccacgccg atcatggaat
      361 tgatcaagcc gcgattgtag aggacgtggc cgatatcgcc gagctccccc gcaccaagct
      421 tcttgccgta gacgtacttt tcgatctcgg cgtgaaccgg gaatttgcca gagccgtgct
      481 gcagcatcaa agccttgtac cctgccgcct gagcgcccgc gggggtgaca tcgggctccg
      541 cacccgacca ccatccgccg atgatcttgt cacggggata ggcaacagcc gcagcctcct
      601 tgatggccgt ggaattcatc acgccccagc cccaaagcag gacgtaatcg ggccggtttt
      661 gccgaacctg cagccactgc gacttctgct ccacacccgg gtgcgtgacc gggatcagca
      721 agagctggaa gccgttcagc tccgagcgct tctgcaacag cggaatcggc tccttgccgt
      781 aggggctatc gtggtagacg agcgcgatct tcttgccctt gagcttgtcc atgccaccga
      841 gctctttggc gatgtgctgg atcgcaacgt ccgcagccgt ccaatacgtg ccaagggcta
      901 tgaaattcca ggtgaatacc gagccgtcgc ggctatcgga gcggccgtag ccgccggtca
      961 gcaatgggat cttgtcgccg ggcgctttct cggtgagcgc gaaggtaatg ccggtcgaga
     1021 gcggagagaa atagccggcg ccggttgggc ctttgccctt gaggcgctca tagcattcaa
     1081 cgcccttgtc ggtcgcataa ccggtttcgc attcctcgaa aacgatcttc accccgttta
     1141 tgccgccgtc gcgctcgttg acgagagtcc agtagtcggc gacgccattg gcgaagggaa
     1201 tgccgtttgg cgcatatggg ccggtcctgt agacgagcgc cggcaggaac tgctcgttct
     1261 gtgcctgtgc cggcatggcc aaagccaagc cggcaaacgc cgcggcggct gccagtcccc
     1321 aactcttgcg agccattctt ctcatcctcc ttggttcggc ccgggcaagc cgggcacctc
     1381 acgcgcgggc tttgttttta gttctggctg cgacctgccg ctccgccctg caggcctgcc
     1441 ttcccttctc gccgctctag taggggaacg gccagagtct cagcttctcc ttcgcaattg
     1501 accacaaccg ggcaaacccg tgtggctcga cgatcagaag aaagcagatc aacgccccgg
     1561 tgatgatgaa cacgagatgc gagacggtct ccaccgacag cggaataccc agcgcgtggg
     1621 gcacctggtc gaggaagatc ggtagaatga gaatgaaagc ggccccgaga aaattgccca
     1681 aaatcgagcc cagcccaccg atgatcacca tgaacagaag ctggaacgag cgatcgatat
     1741 tgaacgtcag cggctcccac gagcccaggt gcacgaaggc ccaaagcgca ccggcaacac
     1801 cgatgatgaa cgacgagatc gcaaaggccg acagctttgc gtaaagcggc cggataccca
     1861 tcagctcggc ggcgatgtcc atgtcgcgga tggccatcca ttgccgcccg atatttccgc
     1921 gggtcaggtt cttggcaatg atcgcaaagc aagtcacgaa cagcagggca agcatgtagc
     1981 gctccgccgg ggtgttcacg acccagccga agaaatcgag cgtcggagca ctgatcgagc
     2041 ccgacggcgt gtagttggtg aaccattgca cgcgtagaaa cagccaatcg aagaagaact
     2101 gggctgcgag cgtggcaacg gcgagataaa gccctttgat cctgaggctt gggatgccga
     2161 aaaaaatgcc gaccagtgct gacatcgcgc caccaaggag gatcgatacc aggaggggca
     2221 gttcgggaat gcgcagcgcg aaattgtagg cggcatacgc gccgattgcc atgaaggcgc
     2281 cgccgccgag cgagatctgc ccgcagtagc ctgtcaggat gttcagtccg atggccgcca
     2341 gcgacagaat tagaaatggg atcagaatcg ctttgaagat gtaatcgtgc ccgaacgcgc
     2401 cgatctcgaa ttcggccaga agaggaactc cgacgaaggc gaaggccagc aggacgccca
     2461 gcgcgatcct gtcctggcgg attggaaaga tcgccagatc ggcggcgtaa gtggtcttga
     2521 actgcccagc ctcgcggtaa agcacagcgc gctgccctcc tcagatccgc tcgatgatgc
     2581 gctcgccgaa cagcccctgc ggccgcacga tgagaacggc aagtgcgagg acgtaggcaa
     2641 accaatactc gatgccgcca ccgatgagcg gcccgagata gatttcagcc agcttctcgc
     2701 cggcgccgat gatgaggccg ccaacgatgg cgccagggat cgacgtgaaa ccgccgatga
     2761 tcaggacggg aagcgccttc agggccagaa aggtgatcga gtactgcacg ccgagcttgg
     2821 tgccccatac ggttgctgcc acaagcgcca ccagccccgc cacgagccag acgacaaacc
     2881 agatccagct gatgggaatt ccgacggact gtgctgccgc atggtcatct gcgacggcgc
     2941 gcagcgcgcg gcccgtcttc gtccactgaa agaaggcggc cagccctgcc accagaattc
     3001 cggcgatcac gccaccccac acatccagct tgctgatcag aatgccaccg ggaaagacct
     3061 cgtccaaaag gatccaggcg tccgtcgggt agagcgccag cgggtagaca tcggacccga
     3121 acagcatttc cgccgcgccc tcgatgacga atgtggcgcc aatcgtcgac ataaatagcg
     3181 tcagctcgtt ttgattgagc agcggcccaa gcacatatcg ctcgatcagc catgcagtga
     3241 tcgccatgat cgcgccggca aacaaaagag cgccgacgac cgcgacttcg aatggcattc
     3301 cattcgcgac cagcagatcg agggaccgca ccagggccag acctgccagc agcaccatgg
     3361 caccttgtgc gaaattgaag acgccggagg ccttgtagat cagcacgaat ccaagggcga
     3421 cgagcgagta cagcacgcct gccaacaatc cgccgaccag cacctcaaag aatgggccca
     3481 gaccttccac tcaatcccct ccagccctca acccagacag cataaaatga ccgtgtgcgg
     3541 tgccgatcca tgatcccgcc cgagcatcat tttgccgagc cgcagctgcg ttttcgacca
     3601 ttcctgcctc gcgcgagagc atactcaacg cgccactcca agataggcgt cgatgacctt
     3661 ctcgtcgttc tggacctcgt ctggcgtccc atctgcgatc ttgacgccgt aatcgagcac
     3721 cacgacccgg tcggacaggt ccatcaccac acccatgtcg tgctcgatga gagcgatggt
     3781 ggtgccgagc tgatcgttca catcgaggat gaaacggctc atgtcctgct tttcctcgag
     3841 attcatgccc gccatgggtt catcgagaag aagcagctcc ggctcggttg cgagcgcacg
     3901 ccccagttcg acacgtttct gaagtccata aggcagtttg ccgacgggcg tttttcgaat
     3961 gtgctcgatc tctaaaaaat cgatgatccg ctcgacgaat tcccgatggc gaatttcctc
     4021 cttctgcgac caaccccagt aaaccgcctg gccgagaatg ccggtgcgca ttttcagcgt
     4081 ccgcccagtc atgatgttgt cgagcgtcga catgcctttg aacagggcga cgttctggaa
     4141 cgtgcgcgcg atgccctgtc tggcggcgat cgacgggcgc atccggtggc ggcgcttgcc
     4201 cttgaaagtg atcgtgcccc gttgcgggtg gtaaaacccg ttgatgatgt tcaacatcga
     4261 ggttttgccc gcgccattgg ggccgatgat ggcgcggatc tcacccttgc gaatatcgaa
     4321 cgagacgtct cggatagcgt gcacaccgcc gaacgaaagg gaggcgtttt cgacggcgag
     4381 cagcacctcc cgctctgccg gtttcggatc ggccgtcgga cgatccagaa ctgcggcctc
     4441 cgcattcatt ccgcggcctc cttcagcgcc tggacctccg gctgatgctc tggcatgtcg
     4501 acgatgcgga catcggcttc gatgatccct ttgcggccgt cctcgaacgt cacctcggtc
     4561 cgaatgtgag cttgcttcga gccgtcgtag agggcgcgga tcagcggcgc atagcgttcg
     4621 gcaatcaggc cgcggcgcac cttctgcgtg cgcgtcagct caccgtcatc ggcatcgagc
     4681 tctttcggca gcacgaggaa acggcggatc tgcgaagagg ccatcttcgg ttccctcgcc
     4741 agcatcgcgt tcacctcgtc cacacgccgc tcgatcatgg cgtacacgtc cgggtgcgcc
     4801 gcgagctcct gatagctcgc gtacacgaca ttgtttctct cggcccagct cccgaccgaa
     4861 acgaggtcga tgttgacgaa catcgtcacg aagtcgaaac catggccgaa ggccacggcc
     4921 tccctcacct cggggaagaa cttcagcttg ttctcgagat atttcggcgc gaacagcgag
     4981 ccgtctttca agcggccgac atccttcgcc ctgtcgatga tgacgagatg gccgcgcgaa
     5041 tcgaaaaagc cggcatcgcc cgtacgcacc caaccgtcag gcgtctttac ggcggcggtc
     5101 gcttcctcgt tcttgtagta gagctggaaa acgccggggc tgcggaacaa aacctcgccg
     5161 ttgtcatcga tcttgatatc gacctccggc gcgggcgggc cgacggtgtc tgcgtagatc
     5221 tctccgtcgg gctgcatggt gacatagacg gtggcttccg tctggccgta gagctgtttg
     5281 agattgatcc cgagcgaacg gtagaatttg aagatttccg gcccgatggc ctcgccggcc
     5341 gtatagccga ttttcagccg gctcaacccg agctgattgc ggagcggccc atagacgagc
     5401 ttgtcgccga tccaatagag aatccgatcc gataggggaa cgggctttcc ttccaggatc
     5461 ggctctccgc agcggcgcgc cacgtccatg aaacggtcga acatccaccg cttgaggcgg
     5521 ccggcatcct gcatgcggac gaagatgtcg gtcagtgccg cctcgaagcg gcgcggcggt
     5581 ccgaaaaaga acgtcggtcc gatctcccgc cggtcgacga cagtcgtctc gatgctctcg
     5641 gggcaggaaa cgcaaaagcc tgcttcataa gcctggccat aggaaaagat atgatcgccc
     5701 acccaggcca tcggcagata ggcgacgatc tcctcttttt ccgagagctt gtcgaaagcg
     5761 ttggcgttcc gcgccgtgcg gatcaggttc tcgaagctga gcaccacgcc cttgggctgc
     5821 cccgtcgtgc cggacgtgta gaggatgacg ctggtgtcgc tgcctttgcc ttgattgatt
     5881 tcgtcgagcc accagtcacc ggcgcccggg tttgccttca attcctcgcg cccgagcctc
     5941 tgcacctcct caaaagcgtg gagccgggtg tgatcgtatt tcttcaggcc gcgggggtcc
     6001 tcgtagacaa tgcgccggag cgtcgggatc tgatcggcga gtgacaggat cttgtcgacc
     6061 tgctcctggt tttcaacgac cgcgaacgcg atctcggcat ggctgagaac gtagaccatc
     6121 tcagcggcca cggcgtcctg gtacacgggc acgggaatgg cgccgatgct ttgcgctgca
     6181 gcaaaggtcc agtaaagctg gggtcggtta tcgccgatga tggcgaccgt ctcgccgcgc
     6241 ttcaggccga gaacataaag gccgatcgcg aatgcccgga cctcgtcgca cacctgcgac
     6301 cacgtccacg tttgccagat tccgaggtcc t
//
LOCUS       DASVDU010000324         3690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1258030, whole genome shotgun sequence.
ACCESSION   DASVDU010000324 DASVDU010000000
VERSION     DASVDU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258030"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VF226_21720"
     CDS             <1..628
                     /locus_tag="VF226_21720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7076668.1"
                     /translation="MGAMSSGFNDTPERASRAYDKDRDGFVIAGGAGVIVLEELEHAK
                     ARGAKIYGELVGYGATSDGFDMVAPSGEGAVRCMRQAMKGLDGKGTGRIDYINPHATA
                     TPIGDVREIEAIREVFGEDIPPISATKSLTGHSLGAIGVQEAIFSLLMMNNGFICESA
                     NIDELDPAFEDIPIVRKRLDGVELNCVMSNSFGFGGTNATLVFRRADS"
     gene            753..1619
                     /gene="fabI"
                     /locus_tag="VF226_21725"
     CDS             753..1619
                     /gene="fabI"
                     /locus_tag="VF226_21725"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX7076669.1"
                     /translation="MTETDIAKPGPLMAGKRGLVMGVANDRSIAWGIARVLAGQGAQL
                     AFTYQGEAFGRRAVPLAKSVGSEIIVSCDVSDLASVDNVFSEIKKTWGSLDFVVHALA
                     FSDRRELSGHYADTSRENFINTMVISCFSFTEVAKRAAELMPNGGSLLTLTYGGSQRW
                     VPSYNVMGVAKAALEASVRYLASDFGPRGIRVNALSAGPMRTLAGSGIADARVIFNYQ
                     RDHSPLRRTPTLDEIGGAALYLLSPLAGAVTGEVHFVDCGYNTVAMPSLEALKEREAD
                     AEAAGRAPAGAD"
     gene            1725..2579
                     /locus_tag="VF226_21730"
     CDS             1725..2579
                     /locus_tag="VF226_21730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX7076670.1"
                     /translation="MIGVFDSGLGGLTVLRALVERFPRLSFVYLGDHANVPYGNRSSD
                     EILDLTRACVEHLFRQDCRLVLLGCNTATAVAARRLQREWLPQSGYVGHNVLGIIAPT
                     VEAATQTPWAVSTPQYPQKYNSELIAVFGTRRTITSDVYPQEIRKRCPRVQVVQQTCP
                     ELAGAIEESRPEAELDQLVAAAADALMQTTGGEAPHRAILGCTHYALIEHLFRRHLPK
                     STRIMSQPVIVADSLEDYLARHPEYADEAPDPSVTLLTTGDPARINDCARVFWPEANH
                     FIRLADKN"
     gene            2723..3019
                     /locus_tag="VF226_21735"
     CDS             2723..3019
                     /locus_tag="VF226_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076671.1"
                     /translation="MKRTAVFAIAGAVFGVGVAVSGVQAAPSALSDLMGATNSQSLVE
                     EVHGRHRACVRWRGWWHRHVGWRNRTVACGPRWRRYRHCFWDRRGRRVCVWRRR"
     gene            complement(3127..>3690)
                     /locus_tag="VF226_21740"
     CDS             complement(3127..>3690)
                     /locus_tag="VF226_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7076672.1"
                     /translation="LMGLVAPRHGHVLVDGCDATDAPTFRRAQSGIAYVPEGRGIFSS
                     LSVAENLAIAERDGVNGGRTWTQERVLDLFPRLAQRMTHRGDQLSGGEQQMLAIGRAL
                     LTNPRLLILDEATEGLAPIIRDEIWRTIALIREAGIATLIVDKTVEAVTRLADRIVII
                     VKGEVAFEGAPATLKADPELMQRHLGV"
BASE COUNT          636 a         1140 c         1225 g          689 t
ORIGIN      
        1 catgggcgcc atgtcgtccg gcttcaacga cacgcccgag cgtgcgagcc gggcctatga
       61 caaggatcgc gacggctttg tgatcgctgg cggagccggg gtgatcgttc tcgaggaact
      121 ggagcacgca aaggcgcggg gtgccaagat ctacggtgag cttgtcggct acggcgcaac
      181 gtcagatggc tttgacatgg tggctccttc gggcgagggc gcggtgcgct gcatgcgtca
      241 ggccatgaag ggcctggacg gcaagggcac cgggcgcatc gactatatca atccgcacgc
      301 gacggcgact cccatcggcg atgtgcgcga gatcgaggca attcgcgagg tattcggaga
      361 ggatatcccg cccatctcgg ccacgaagtc gctgacgggt cattctcttg gcgcaatcgg
      421 tgttcaggag gcgatcttct ctctccttat gatgaacaac ggtttcattt gcgagagcgc
      481 caatatcgac gagctggacc ccgctttcga ggacattccg atcgtgcgga aacggcttga
      541 cggagttgag ctcaactgtg tcatgtcaaa cagcttcggg ttcggcggca cgaatgccac
      601 gttggtgttc cgccgtgcgg acagttagca atttctctcg agccgcgtcg ttacgtgggg
      661 cacaaacccc ttcggggctg cgcggctatg acttcaaaac aggccgttcg aaatttcatg
      721 cgcaagccgc gcgcccttga tcaggagcac ctatgactga gaccgacata gccaagccag
      781 gtccgctgat ggcgggcaag cggggcctcg tcatgggcgt cgcgaacgac cgctccattg
      841 cctggggcat cgcacgggtg ctggccggac agggggcgca actcgcgttc acctatcagg
      901 gcgaggcttt cgggcgtcgc gccgtgccgc tggcgaagtc ggtcgggtcg gagatcatcg
      961 tctcctgcga cgtgagtgat ctggcgagcg tcgacaacgt gttttccgag atcaagaaga
     1021 cctggggcag cctcgacttc gtcgtccacg cgctggcctt ctccgatcgg cgcgagctgt
     1081 ccggtcatta cgcggacacg agccgcgaaa acttcatcaa tacgatggtg atctcctgct
     1141 tctcgttcac ggaggtggcg aagcgggccg cggagctgat gccgaacggt ggttcgctgc
     1201 tgacgctgac ctacggcggt tcgcagcgct gggtgccatc ctacaacgtg atgggggtcg
     1261 ccaaggcggc cctcgaagcc tccgtgcgct atctcgcgtc cgacttcggg ccgcgtggga
     1321 ttcgggtgaa cgcgctttcg gcggggccca tgcggacgct ggccggttcc ggcatcgcgg
     1381 atgcgcgcgt catcttcaat tatcagcgcg accactctcc gctgcggcgc acgccgaccc
     1441 tcgatgagat cggcggagcg gcactctatc tgctctcccc actggcgggc gccgttacgg
     1501 gcgaagtcca tttcgtggac tgcggctaca acaccgtcgc gatgccgagc ctcgaggcgc
     1561 tgaaagagcg ggaggccgat gcggaagcgg ccggccgcgc gccagccggc gccgactgag
     1621 ccagcgcccg tcttgcgcca ttggctgctg cctcacgggc tcgcctcatc aaaatggcct
     1681 gagcttgttg cggttgcacg tccggcgtgg catggcttga agccatgatt ggtgtattcg
     1741 actccggcct cggtgggctg acagtgctgc gggcgctggt cgagcggttc ccacggctgt
     1801 cgttcgttta cctcggcgat cacgccaatg tgccctacgg caaccgctcg tcagatgaaa
     1861 tcctggattt gacccgtgcc tgcgtcgagc accttttccg gcaggactgc cggctcgtgc
     1921 ttctcggctg caacacggcg actgctgtcg ccgcgcggcg gctgcagcgg gaatggctgc
     1981 cgcagtccgg ctatgtgggc cacaatgtcc ttggcatcat cgcgccgacc gtggaagcgg
     2041 caacgcagac gccttgggct gtttcgacac cgcagtatcc gcagaagtac aactccgagc
     2101 tgatcgcggt gttcggcacg agacgcacga tcacgtctga tgtttacccg caggagatcc
     2161 gcaagcgctg cccgcgcgtc caagtggtgc aacagacctg tcccgagctg gctggcgcca
     2221 tagaggagag cagaccggaa gctgagttgg atcagctggt ggcagcggct gccgacgcgc
     2281 ttatgcagac gacgggcgga gaggcaccac atcgcgccat ccttggttgc acgcactatg
     2341 ctttgatcga gcacctgttc cgccggcatc tgccgaagtc gacccgcatc atgtcccagc
     2401 cggtgatcgt ggccgacagc ctggaggatt atctggcccg ccacccggag tacgcggacg
     2461 aggctcccga tcccagtgtc acactactga caacgggcga tccggcccgg atcaatgatt
     2521 gcgcccgggt tttttggccg gaagccaatc atttcattcg gttagctgac aaaaattagc
     2581 agacatactc tcttgttgtc gtaagtagct gatgcgccgg gagaataccg ccgcatggct
     2641 gccaacggcc atcccctctg aaacctctca gtcgaacaac gggttatggc cgcagcaact
     2701 gtggtcaatc gtgagaggtc aaatgaaacg gacagccgtt tttgccatag ccggtgcagt
     2761 attcggggtg ggcgttgccg tttccggcgt gcaggcagcg ccgagcgcgc tatccgattt
     2821 gatgggcgcc accaactccc aatcgcttgt cgaggaggtc catggccggc accgggcctg
     2881 tgtccgctgg cgcggctggt ggcatcgcca cgtcggctgg cgtaaccgca ccgttgcatg
     2941 tgggccgcgc tggcgcaggt acaggcactg cttctgggat cggcgcggca ggcgcgtttg
     3001 cgtgtggcgg cggcgctgag gctcggatcg ttcgcgtaac gcgaaatggg ctggtgccac
     3061 acccaccagc cccattcagt catgattgcg ccacccccgc ggaaaacgac ccgaggcggg
     3121 ttgaaatcaa accccgagat gccgttgcat cagctccggg tcagccttga gcgtcgcggg
     3181 cgcgccttcg aaggcgacct cgcctttgac gatgatgacg atgcgatcag cgaggcgcgt
     3241 gacggcctcg accgtcttgt cgacgatcag cgtggctatg ccagcctcgc ggatcagggc
     3301 gatggtccgc cagatctcgt cgcggatgat cggcgccagt ccctcggtcg cctcatcgag
     3361 gatcagcaaa cgcggattgg tgagcagcgc gcggccgatg gcgagcatct gttgctcacc
     3421 gccggagagc tggtcaccgc ggtgagtcat gcgctgcgcc agccgcggga acaggtcgag
     3481 gacgcgctcc tgcgtccagg tccggccgcc gttgacgccg tcgcgctcgg cgatggcgag
     3541 gttttcggca accgacagcg acgagaagat gccacggccc tcggggacat aagcgatgcc
     3601 ggattgcgcg cggcggaagg tgggcgcgtc cgtcgcatcg cagccgtcga cgagcacgtg
     3661 cccgtgccgc ggggcgacaa ggcccatcag
//
LOCUS       DASVDU010000325         5112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1066130, whole genome shotgun sequence.
ACCESSION   DASVDU010000325 DASVDU010000000
VERSION     DASVDU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066130"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VF226_21745"
     CDS             <1..1038
                     /locus_tag="VF226_21745"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7076673.1"
                     /translation="GHQDSWFGEAATDNPSGNACKMELARVFYRHRDKLRRGLMFGFW
                     AAHETGTMAGSAWFCDRHWDELREHCIAYLQIDQPAIIGTTEWNTASNAELKVFHTEI
                     ESRLLDCPIRWHRAVKNGDSSFFGIGIPMFQGEGAYTEAELKETALASFGWWHHSIEN
                     RLDKIDWVAMQKHIQVYAAYLWRLCTEPVLPFRFTPVADELIARLEQLREAGASIGLD
                     TALSPARRLREQAQALDAAAEKEAERFRAGTGSEDAALLLNGAMKRLSRILVPLQSTA
                     IGTYGHDPYGYTPQTTVIPCLYDVPKLARLPDGPERWMLETALVRARNRVADALSDAA
                     DVISVALARMS"
     gene            complement(1161..3029)
                     /locus_tag="VF226_21750"
     CDS             complement(1161..3029)
                     /locus_tag="VF226_21750"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX7076674.1"
                     /translation="MKNSNIVLIDRHPGRSTQSIGMARELGTDPELIHEPSVGVVGTK
                     GDSQCYLGVSSKVEAIHARLRSRIGKGPGQLKLRLVQPEFTIATSDGIRNGTREMRYS
                     LIGREVTHDALCEHLSATGLAGTIAVVACDKPPVGTLAALLEHNQPAIIMSDGPIHPG
                     TDPVTGEKIDIVSAYQVAGHPDAAYRERIACHACGGIGSCGGMFTYNTMQTFIGVVGM
                     QPLHMVAPASDDPRRLNEFPAELVEYLANLMEKDLKPRDIVVRDSIRNAVIVAMAIGG
                     STNVALHAPEIARAAGYADFWKDIMTPEEFNHLSQHVVPVLTDARPYGTYSMVDIDQV
                     GGVQVIVRELLEAGLLNGDVMTCTGETLAQQVDRLGAKRPDGKVIYPVEKPFKPTGGL
                     RVLGGNLSPDFSAILKLAGVEAGLENNVFRGRARVFEGEQDLLAALDATPERFQNNDM
                     IVVRYEGPSGAPGMPEMLDPTSRITALCRERGIVVALMTDARFSGGSVGLVIGHVGPE
                     AAIGGPIAFIEDGDEIVVDLNRNEINCTPLSDPAVYAKRKAAWEKVVADNGGIHPNCG
                     EADTRLLHRARHTAVPATRGGGMHPNREVWVRNPRPAERSGFVPRNKYRPEATKAF"
     gene            3474..4469
                     /locus_tag="VF226_21755"
     CDS             3474..4469
                     /locus_tag="VF226_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076675.1"
                     /translation="MWGALIVGWALSIGLGLLANAAAAQPSKLSAEHIKETFAGAILE
                     LDTPLGTKVPIRFSSSGLMAGEAGPLGSYLGADRDRGRWWVADDRLCMKWFRWFEAGT
                     HCFDLQQDGEQIWWQEQSGKSGTARIAGRFEEKPTGTEVASAAKSHAVQQRRETITRS
                     SEPQRAEASVAQESVSPRAAAHGAEGGGPDRVQSFVQAGALMASVAIPKPRPARPHPP
                     PATPASRLAEAPPLPVRPSRRPALASERPSVEMEAATYRVTRVSSDDILNVRNGPSEY
                     HMPVGFIEPNGRGISIVGACQGMWCPIRYGRVRGWVNSYYLAKEQASREVLAGAR"
     gene            complement(4486..>5112)
                     /locus_tag="VF226_21760"
     CDS             complement(4486..>5112)
                     /locus_tag="VF226_21760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX7076676.1"
                     /translation="GLTEIAAPFLKQKGWQGEAHRFVTWWRRTHFENSMIDALCDRGH
                     TPYRQIGHRAVSYVMDRCGISYTQDEVRWLVSEIEKLKPFPDVVEALNKLRGAGYRLV
                     ILSNGDRDMLEAAKPHIGFPFDLVISVQEAGYFKPHWKTYAKAEELIGLDRSSCLFVA
                     NHAFDCIGAKSYGMRTAFIDRRKRPFGETPHQPDLIVADFAELAATLC"
BASE COUNT          876 a         1608 c         1707 g          921 t
ORIGIN      
        1 gggcatcagg acagctggtt cggtgaggcg gcaacggaca atccctccgg caacgcctgc
       61 aagatggagc ttgcccgcgt cttctaccgg catcgggaca agctgcgccg cgggctgatg
      121 ttcggcttct gggcggcgca cgagaccggc accatggccg gatcggcctg gttctgcgat
      181 cgccattggg acgagctgcg cgagcactgc atcgcctacc tccagatcga ccagccggcc
      241 atcatcggca ccacggagtg gaataccgca tcgaatgccg agctgaaggt gtttcatacc
      301 gaaatagaat cacggctgct cgattgcccc atccgctggc accgcgcggt gaagaacgga
      361 gattccagct tcttcggcat cggcatcccg atgttccagg gtgaaggcgc ctataccgag
      421 gcggagctga aagagacggc gctcgccagc ttcggctggt ggcaccattc catcgagaac
      481 cgactcgaca agatcgactg ggtcgcgatg cagaagcaca ttcaggtcta tgccgcctac
      541 ctctggcggc tatgcacaga gccggtgctg ccattccggt tcacgcccgt tgctgatgag
      601 ctgattgcgc gactcgagca gcttcgggag gcaggggcca gcatcggcct cgataccgcg
      661 ctcagccccg cccggcgcct tcgtgagcaa gcacaggcgc tcgatgcggc agctgaaaag
      721 gaagccgagc gcttccgtgc cggcaccggc tcagaggacg cggccctgct cctcaacggc
      781 gcgatgaagc ggctgtcccg cattctcgtg ccgttgcagt cgacggcgat cggcacctac
      841 ggtcacgatc cctacggcta taccccgcaa acgaccgtca ttccgtgcct ctacgacgta
      901 ccgaaacttg cgcgcctgcc ggacggaccc gagcgctgga tgctggagac cgcccttgtt
      961 cgggcccgca accgggtcgc cgatgcgctg agcgatgcgg ccgatgtgat ctcggtggcg
     1021 ctggcccgta tgagctagct ctgctttcgg ctcaggcagg agaggccgac cgtccagtgg
     1081 agctcaacac cagctcgacc ggcgcgtacg cccgcccgta gggtagatgg gcggcccccg
     1141 gtgggagccg ccggtgagag ttaaaaagct ttggtcgctt ccggacggta tttgttccgc
     1201 ggcacgaagc cggagcgttc ggccggacgt gggttgcgaa cccagacctc gcggttcgga
     1261 tgcatcccgc cgccgcgcgt ggccgggacc gcggtgtgcc gggcgcgatg aagcaggcgc
     1321 gtgtccgcct ccccgcagtt cggatgaatg ccgccgttgt ccgcaaccac cttctcccag
     1381 gcggccttgc gctttgcgta aacggcagga tcgctgagcg gcgtgcagtt gatctcgttg
     1441 cggttcaggt cgacgacgat ctcgtcgccg tcctcgatga aggcgatcgg gccgccgatg
     1501 gcagcttccg ggccaacgtg gccgatcacg agcccgacgg aaccgccgga gaagcgggca
     1561 tccgtcatca gagctacaac gatccccctc tcacggcaga gcgccgtaat gcgcgaggtc
     1621 ggatcgagca tctccggcat ccccggagcg ccgctcgggc cctcatagcg aacgacgatc
     1681 atgtcgttgt tctggaaccg ctcgggcgtg gcgtcgagcg ccgcgagcag gtcctgctcg
     1741 ccctcgaaga cgcgggcgcg gccgcggaaa acgttgttct cgaggccggc ctcgacgccc
     1801 gccagcttga ggatcgccga gaagtccggg gacaggttgc cgccgagcac cctcagacca
     1861 ccggtcggct tgaagggctt ctcgacggga tagatcacct tgccgtcagg gcgcttcgcg
     1921 cccagacgat cgacctgctg ggcaagcgtc tcgcctgtgc aggtcatgac atcgccgttc
     1981 agcagccccg cttccaggag ctcgcggacg atgacctgga caccgccgac ctggtcgatg
     2041 tcgaccatgg agtaggtgcc ataaggccgg gcatccgtga gcacggggac gacgtgctgc
     2101 gagaggtgat tgaactcctc cggcgtcatg atgtccttcc agaaatcggc gtagccggcg
     2161 gcgcgcgcga tctcgggcgc atgcagcgcg acattggtcg agccgccgat ggccatcgcg
     2221 acgatcaccg cgttgcggat ggaatcgcgc acgacgatgt cgcgcggttt caagtccttc
     2281 tccatcagat tggcgaggta ttcgacgagc tcggccggaa actcgttcag cctgcgcgga
     2341 tcgtccgaag ctggtgcaac catgtgcagg ggctgcatgc cgacgacccc gatgaaggtc
     2401 tgcatggtgt tataggtgaa catgccgccg cagctgccga tcccgccgca ggcgtggcag
     2461 gcgatccgct cgcgataggc ggcatcgggg tgccctgcaa cctggtaggc gctcacgatg
     2521 tcgatcttct ctccggtcac gggatccgtg ccgggatgaa tcgggccgtc cgacatgatg
     2581 atggccggct ggttgtgctc gagcagcgcc gcgagcgtgc cgacaggcgg cttgtcgcag
     2641 gcaacgactg cgatcgtgcc ggccaggccc gtcgcactca gatgctcgca gagggcgtcg
     2701 tgggtgactt cgcgtccgat cagcgaatag cgcatctcgc gcgtgccgtt gcggatgccg
     2761 tcggaggtgg cgatggtgaa ctccggttgg acgaggcgca gcttgagctg gcccggtccc
     2821 ttgccgatgc ggcttctgag ccgggcatgg attgcctcca ccttggacga gacgccgagg
     2881 tagcattggc tgtcgccttt cgtcccgacc acgccgacgg aaggctcgtg gatgagctct
     2941 ggatcggttc caagctcgcg cgccattccg atggactggg tcgagcggcc gggatggcgg
     3001 tcgatgagaa cgatattcga gttcttcact ggaggctcct tccaacagcg cagcgcgttc
     3061 ggtccatccg gagaatagct tggctgattt cccgatcgga aggacattat ggcgggcgaa
     3121 ccgtcgcgcc aagcgccgct gctctcgccg gcgggacagg tgcgaggctg ccgtggcgtc
     3181 gacaatggca aggctcagca gccgatggcc tccgcgcgct tccgcctgag ttctgagttc
     3241 attcaactcg ggcgttgatt cgcttgtctc tcagccgcct cacggctgcg atcagagtcc
     3301 accccgaagg cctcgaggga ggcgcggatc caggccggga cggcgttgcc tactccataa
     3361 gcgtatagcc cgagctgcac gcgcgcgccg gaatgaatct catcggtgtt cctcttgctg
     3421 ccacagtccc tgttcacgga taggggtgtt catgcgagtg gggggacatg tgcatgtggg
     3481 gtgctttgat tgtcggctgg gcgttgtcca tcggcctcgg tctgttggcg aacgctgctg
     3541 cggcccagcc atcgaagctg tccgctgaac acatcaagga aacattcgcc ggtgccatcc
     3601 tcgagctcga tacgcccctc ggcacgaagg tcccgatacg cttttccagc agtggtctga
     3661 tggccggtga ggccggcccc ctgggctcat atcttggggc ggatcgcgat agagggcgct
     3721 ggtgggttgc ggatgaccgc ctctgcatga agtggttcag gtggttcgag gccggcacgc
     3781 attgtttcga cctgcagcag gatggcgagc agatctggtg gcaggagcag agcgggaaga
     3841 gcggcacggc gagaatcgcc ggccgtttcg aggagaagcc cacaggaact gaggtcgcat
     3901 cggctgcgaa atcgcacgct gtccagcaaa ggcgcgaaac gatcaccagg tcttccgagc
     3961 cgcagcgcgc cgaggcgtcc gttgcgcaag agtccgtgag ccctcgggcc gccgctcacg
     4021 gtgccgaggg tggcggacct gatcgggtgc agagctttgt ccaagcggga gctctgatgg
     4081 cctcggttgc cattcccaaa cctcggcccg caagacctca tccgcctcct gcaacacccg
     4141 cctcgagatt ggcggaagcg ccgccgttac ccgtccggcc atcgaggcgc cctgctttgg
     4201 cgtcagagag gccctcggtg gagatggagg ctgcaaccta tcgcgtcacg cgcgtcagca
     4261 gcgacgacat cctcaacgtg cgcaacggcc cgtcggagta tcacatgccg gtcggcttca
     4321 tagagccgaa tggtcggggt atttcgatcg tcggagcctg ccaggggatg tggtgcccga
     4381 tccggtatgg acgtgtccgc ggctgggtca actcctatta cctcgccaag gagcaggcga
     4441 gtcgggaagt cttggccggg gctcgatgag ccccagctgc cacgatcaac acagcgtggc
     4501 tgcaagctcc gcgaagtccg cgacgatgag atcgggctga tgcggtgtct cgccgaacgg
     4561 gcgtttgcgg cggtcgatga aggccgtccg catgccgtat gacttcgcgc cgatgcagtc
     4621 gaaggcgtga ttggcaacga acaggcagct cgagcggtcg aggccgatga gctcctcggc
     4681 cttcgcgtaa gtcttccagt gcggcttgaa atagccggct tcctgcaccg agatcaccag
     4741 atcgaagggg aagccgatgt gcggcttggc cgcttccagc atgtcgcggt cgccgttgga
     4801 gaggatgacg agcctgtagc ctgcgccgcg cagcttgttg agagcttcga cgacgtccgg
     4861 aaacggcttc agcttctcga tttctgaaac gagccaacgc acctcgtcct gcgtgtagct
     4921 gatcccgcaa cggtccatga cataggagac ggcccggtgg ccgatctgcc ggtagggtgt
     4981 gtgcccgcga tcacaaagcg cgtcgatcat ggaattttcg aagtgcgtgc gccgccacca
     5041 cgtcacgaac ctgtgggcct cgccctgcca gcccttctgc ttgagaaacg gtgcggcgat
     5101 ctccgtcagc cc
//
LOCUS       DASVDU010000326        12411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1163515, whole genome shotgun sequence.
ACCESSION   DASVDU010000326 DASVDU010000000
VERSION     DASVDU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12411
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163515"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1249
                     /locus_tag="VF226_21765"
     CDS             437..1249
                     /locus_tag="VF226_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076677.1"
                     /translation="MTSAAAILSVNNIEVIYDHVILVLKGVSLKVPEGGVVALLGANG
                     AGKSTTLKAISNLIRSERGEVTKGTIEFNGERVDGLTPNDLVRRGCIQVMEGRHCFGH
                     LTVEENLLTGAFTRRDGGGAIQQDLEKVYSYFPRLKERRNSLAGYTSGGEQQMTAIGR
                     ALMSRPRMILLDEPSMGLAPQIVEEIFGIVHNLNQREGVSFLLAEQNTNIALKYATYG
                     YILENGRIVLDGAAEELRENEDVKEFYLGVGGGHRRSFREGKSYKRRKRWLA"
     gene            1417..2655
                     /locus_tag="VF226_21770"
     CDS             1417..2655
                     /locus_tag="VF226_21770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7076678.1"
                     /translation="MTSAHFDQLETRDPEQREIALFNLLPSVIRRAKELAPGWARHLA
                     DVDPDAVTSREALAKLPILRKSSLPELQIAQLPFGGFATAAPSEMARIYMSPGPILEP
                     EGFGEDWWRTARALFAAGFRKGDIVHNTFSYHLTPGAWILDAGARALGCTVVAAGPGN
                     TEQQLEAIARLNPTAYVGVPDYLKILLDKAREAGREIKSFKRAVVSGGALFPSLRQEY
                     RDRGIEVFQMYATADVGVIAYESSALDGMIVDEGVIVEIVQPGTGDPLPPGEVGEVVV
                     TSFNRDYPMIRLATGDLSAVIEGVSPCGRTNMRIKGWLGRADQTTKVKGMFVHPGQVA
                     TVAKRYPELGRVRLVVGRSGEQDTMVLKAESKSQDPALAARVAETLQDVTKLKGVVEL
                     VGPGTLPNDGKVIVDERTYE"
     gene            complement(2762..4291)
                     /locus_tag="VF226_21775"
     CDS             complement(2762..4291)
                     /locus_tag="VF226_21775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003588150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEX7076679.1"
                     /translation="MAIEGNLYAALEARFRAAGSAIAFSIPGGADVSYASLADDVARM
                     ANALVSLGVKPGDRVLAQVEKSLANVHLYLATLKIGAVFNPLNTAYTAAELDYFINDA
                     EPTLIVVPNEKLPRVESIAAALKTAKVVTLEADGSGSLSDLARQQSAEHETVARDSED
                     LAALLYTSGTTGRSKGAMITHRNLSSNAEVLHSYWGFEPGDVLLHALPIFHTHGLFVA
                     LNTAFLNSSRVIWLPKFELESVMRLMPEATVMMGVPTFYTRLLGNPSFGREHCARMRL
                     IISGSAPLLAETHQEFEARTGHKILERYGMTEAGMITSNPYKGGERIAGTVGYALPGI
                     SVRIATPEGEPVPPGEVGVLEVKGPNVFKGYWRNPEKTREEFRPDGYFISGDLATMSE
                     DGRVTIVGRAKDLIISGGFNVYPKEIEQELNALPGVSESAVIGVPHPDFGEGVVAVLT
                     PQPGATLPSETEVIARLSERLAKFKLPKRVFVTDELPRNAMGKVQKAELRKQFANTFS
                     G"
     gene            complement(4443..5741)
                     /locus_tag="VF226_21780"
     CDS             complement(4443..5741)
                     /locus_tag="VF226_21780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7076680.1"
                     /translation="MAGLLRPVTALLIAAAILLMGNGLLGVLIPVRADVEQFSRLEIG
                     LLGSFYYAGLVAGCLLGPKVIARVGHIRGFVAFTAIVTITPLLHAIWPEPIAWSLLRA
                     LNGLCFAGLFMGIESWLCGASTMETRGRVLASYTVVNLTVVTVGMQMMAIGSPGGFEL
                     FSLVAILYSLAAVPVALTKTSAPIPPKTARVRPVWLFNISPAAVAGCFFTGVANSAFW
                     ALAPLYGRSAGFSLAEVAMFMTVAVLGGAASQWPIGYLSDRAGRRPLLVTLGTTGAFA
                     GAGLYILSTSSPMAIFPMALLYGCCAFPIYTLCVAHANDLVHKKRAVEVSSGLLLTFS
                     IGAVLGPLMAGFVMSEGGHGALFLHTAAAHLLIALVMLIRVKLRPKLPAEHKEGFVAV
                     PKTTPTVFELDPRAEGTAAAAYSADMVTEELSERAAGGQS"
     gene            complement(5788..6240)
                     /locus_tag="VF226_21785"
     CDS             complement(5788..6240)
                     /locus_tag="VF226_21785"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7076681.1"
                     /translation="MDLPDIIHFRNLETMPPPKTPLLTVDCVVFNPRGEVLLIERKNE
                     PFKGHLALPGGFVDVGETVEDACRRELIEETGVKAGRLTLVGVYSDPKRDPRGHTASV
                     VFLTRVRSSKVQAGDDAAAARWVADWRRLPLAFDHAKILADATRMAKC"
     gene            complement(6280..9771)
                     /locus_tag="VF226_21790"
     CDS             complement(6280..9771)
                     /locus_tag="VF226_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX7076682.1"
                     /translation="MPKRAVSLSDKFDLSETRQILTGTQAIVRLALMQQVRDRQAGLN
                     TAGYVTGYRGSPIASLEGAFLRARSILDPHNIHFHPGLNEDLAATALWGSQQAELRGE
                     GKYDGVFGIWYGKGPGVDRTGDVFRHANHAGTSKYGGVLALLGDDHTCESSTSAHQSE
                     FAMMDAMIPVLNPAGVQEILDFGLLGFALSRYAGVWVALKCVKDNIESTATVDGRIDR
                     VKVVIPSEDEFRMPPDGLNIRLNDQPLAKEARLHDFKLPAILAFSRANKLDRLVMSGG
                     PAPKLGIITTGKSYLDVRQALDELGIDEVKANELGIRIYKVGVVWPLEPRGIASFAEG
                     LDTIMVVEEKRSLIETQVKEQLFDAPNHPRVIGKNDENGQPLFPAKGALEPTDIAIAI
                     GERLIRWTLGGEALKAKVAELKQLKGNRPSQPEAFLRTPYFCAGCPHNSSTVVPEGMR
                     AYAGIGCHYMAQWMDRSTEGFTQMGGEGANWIGEAPFSKRKHVFQNLGDGTYLHSGSL
                     AIRAAMAAGTNITFKILYNDAVAMTGGQKLDGGIGVPQIAQQLLAEGVRRVEVVSDEP
                     DKYKGAYSLPNGVTAHHRRELMAVQRSLAETEGVTALIYDQTCAAEKRRRRKRGEFPD
                     PDKRVFINPEVCEGCGDCGKKSNCVAVVPLETELGRKRAIDQSACNKDFSCIEGFCPS
                     FVMLHGAKVRKSKGAASGNGGLDELVASLPEPKAPSLNGPYSMLVTGVGGTGVVTVSA
                     VLGQAAHIEGKGFGSIDMTGLAQKGGAVACHMRVARTADEIHAIRIGVANADLVLGGD
                     LVVTASNKILETIRPDETAVVVSTHEMITGDFTRNPKLTVPGDQLLQAIADRVRKGPL
                     HTFDAHDYAVKLFGDSIASNIFLLGYAYQLGYVPIGAAAIEQAIVLNGASVDMNRSAF
                     RLGRLAAHDKAAVDRVAKPADQHASEPETLDGLVAFRARQLAAYQDEELASRYRAKVA
                     WIAGVENEKAPGRSGLATAVARAYHKLLAYKDEYEVARLFTDGAFQRQVNEQFEGVRA
                     MEFYLAPPILSGFFKDKTTGHPRKIRLGGWMLPVFRLLAKGKKLRGTRWDVFGYSAER
                     KLERQMIADYERLLDDLAANLNQGNHGIAVALAELPLEIKGFGHVKLANYKAAKAREA
                     DLLQAFSNSAPVKLAAE"
     gene            complement(10002..11042)
                     /locus_tag="VF226_21795"
     CDS             complement(10002..11042)
                     /locus_tag="VF226_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076683.1"
                     /translation="MDRRTFLKSTTVAAGSGLALSSTPVLAGADPDPLAAPALSRGTI
                     ELTFAAAWEPDVPIFGDAAQRMLTRLGQLLDGRCHLVAVEASEEADLVFGPVSVEQDP
                     AFAFFCGLPGSYGLDLAHLQAWLVAGGGQMLWDDLAARHGLKPLLAGHTGQSPGLWTA
                     CPIRSAADLAGVSLAVGGPGASVASKLGAIPCEWPASQLRDGLANRELTAVEWGNPLA
                     GLMLGLPQVATHFHIGGIHRNGMAIAMHVRMAIWERLDQSTRLALEALAAHELAVSSA
                     ESLSHACLAEQVITAMPDLEVVEFPPMLATAIDQATAALIEEIGVTSPDSARIRDSYV
                     QYHRQVHSPQLV"
     gene            complement(11138..11533)
                     /locus_tag="VF226_21800"
     CDS             complement(11138..11533)
                     /locus_tag="VF226_21800"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEX7076684.1"
                     /translation="MSRSQFIMLTLAAVVAGATLGALTAPASKKDMRRGQVSRELVVF
                     ETADCVYCRLFRRDVLPEYRNSGRSSELPIRFVDVNDAGPLESALSGPLTIVPTAVLL
                     IDGREAGRITGYTGQASFQQIIGRMLAAN"
     gene            11819..>12411
                     /gene="dctP"
                     /locus_tag="VF226_21805"
     CDS             11819..>12411
                     /gene="dctP"
                     /locus_tag="VF226_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX7076685.1"
                     /translation="MSISRRTFIATAGAGIAAPAVLRVTRANAAEVTLRLHHFLPPVA
                     SVPKHMLEPWAKRIESGTGGRLKIELYPSMQLGGKPPQLYDQAKDGVADIVWTLPGYT
                     AGRFPSTEVFELPFVAARTARVNARAAQEFADQHLKEETKDVKLLCFWCHDHGLIHAK
                     RQVKTMEDLKGLKLRFPTRLAGEALEALGASPIGMPVP"
BASE COUNT         2344 a         3854 c         3974 g         2239 t
ORIGIN      
        1 cctggcacgt gatctctgac tggtacgagg gtgacgactc tctcctggct ccgctggtca
       61 aggagaccgc agcggcttac gccaaggaaa aggggatcac cccgcgtgat tgctcgaagg
      121 ttgagtagga gcgaatagcg aatggcgcgt agcgaatagg tgatggcact gcggtttctt
      181 ttggcctagt cccaatctat ttcgctattc gccgttcggc tcgcatttct gagttggatg
      241 gaaacaggct aggcaagctc cgcgcatttc tccctctccc cagtggggag agggttgggg
      301 tgagggggtt ccgccagtgg ccatgttgca acccctcacc cggcccttcg ggccgacctc
      361 tccctatggg agaggtgaag ggagtggcag atgccgtctt ttccacctga ttccgaaaag
      421 gtttacggtg aatgccatga cgtccgctgc ggccatcctc tcggtcaaca acatcgaggt
      481 gatctacgac cacgtcatcc tcgtgctgaa aggcgtgtcg ttgaaagtgc cggaaggcgg
      541 ggtcgtggca ttgctcggtg cgaacggtgc cggcaagtcc acgacgctca aggcaatctc
      601 gaaccttatt cggtcggagc ggggtgaggt caccaagggc acgatcgagt tcaacggcga
      661 gcgggtcgac ggcctgacgc caaacgatct ggtccggcgc ggctgcatcc aggtcatgga
      721 aggcaggcat tgcttcggac acctcaccgt cgaggaaaac ctgttgacgg gagctttcac
      781 gcggcgcgat ggcggcggcg ccattcagca ggatctcgag aaagtctaca gctacttccc
      841 gcggctcaag gagcgccgca acagcctcgc cggctacaca tccggcggcg agcagcagat
      901 gaccgccatc ggccgtgcgc tcatgtcgcg gcccagaatg attctgctcg atgagccgtc
      961 gatgggtctc gcaccgcaga tcgtggagga gattttcggt atcgttcaca atctgaacca
     1021 gcgcgaaggc gtcagcttcc tgctggcgga gcagaacacg aatatcgccc tcaagtacgc
     1081 cacctatggc tacattctgg agaatggccg tatcgtgctc gacggcgcgg ccgaggagct
     1141 gcgcgagaac gaggacgtga aggagttcta tctcggcgtc ggcggcgggc atcggaggag
     1201 cttccgcgaa gggaagtcct acaagcggcg gaagcgctgg ctggcctgag caggctcctg
     1261 tccccaggat cgcccctagt tcttttgggg gcacccgcgc ccattggtgg tcgctgtagc
     1321 gcgacgagag gcgggctggt gatcccgcgc aacatgcttt agcaccgtca catgggataa
     1381 tccgaccagt cgctgattcc catcttcgag gagatcatga cgtctgcgca tttcgatcag
     1441 ctcgagaccc gcgaccccga gcagcgggaa atcgctttgt tcaacctgct tccgagcgtc
     1501 attcggcgtg ccaaggagct tgcgcccggt tgggcgcggc acctggccga tgtcgatcca
     1561 gacgccgtca ccagccggga agcgctggcc aagctgccca tcctccgcaa gtcgtctctc
     1621 ccagagctgc agatcgccca gttgccgttt ggagggtttg ctacggcggc tccctcggaa
     1681 atggcgcgca tctacatgtc gccgggcccg attctggagc ccgaagggtt cggtgaggac
     1741 tggtggcgaa cggcgcgggc cctgttcgcc gcgggcttcc gcaagggcga catcgtccat
     1801 aacacgttct cctatcacct tacgccgggt gcctggatct tggacgcggg agcgcgcgcg
     1861 ctcggctgca cggtagttgc ggcagggccc ggcaacacgg agcagcagct cgaggccatt
     1921 gcccggctga acccgaccgc atacgtcggc gtgccggatt atctcaagat cctcctcgac
     1981 aaggccaggg aagccgggcg ggagatcaag tcgttcaaac gcgcggtggt atcgggcggc
     2041 gcgctctttc cttcgttgcg gcaggaatac cgggatcgcg gcatcgaggt cttccagatg
     2101 tacgcgacgg cagacgtcgg cgtcatcgct tacgagagct ccgcgctcga tggcatgatc
     2161 gtggacgaag gcgtcatcgt cgaaattgtg cagccgggca caggcgatcc gctgccgccg
     2221 ggagaagtgg gcgaagtcgt ggtgacctcg ttcaatcgtg actatcccat gatccgcctg
     2281 gcgactggcg atttgtccgc cgtcatagag ggtgtcagtc cctgcggccg tacgaacatg
     2341 cgcatcaaag gctggctcgg ccgcgctgac cagacgacga aggtcaaagg catgttcgtg
     2401 cacccggggc aggtagcgac ggtcgcaaaa cgctaccccg agcttggccg ggtccgtcta
     2461 gtcgtcggac ggagcgggga gcaggacacg atggtcttga aggccgagtc caaatcgcag
     2521 gatccggcac ttgccgcccg cgtggccgag accctgcagg acgtgacgaa actgaagggt
     2581 gtcgtcgagc tggtgggacc cggcactctg cccaatgacg gtaaggtcat tgtggacgag
     2641 cgcacctacg aataggagtt ccggctcgcg tcttcccaaa cccccgccgg atggccggga
     2701 agtggtgaac tggagtggtg attccaaaga tcaccactcc ggacttgagc gaaacctcgg
     2761 gctatcccga aaacgtgtta gcgaactgct tccgcaattc ggctttctgc accttgccca
     2821 tggcgttgcg cggcagctcg tccgtcacga aaacccgctt cgggagcttg aacttggcaa
     2881 ggcgttcgga cagtctggcg atcacctccg tttcgctcgg gagcgtcgcg ccgggttgag
     2941 gggttaggac tgctacgact ccttcgccga aatcggggtg cggaacacct attacggcgg
     3001 attcgctgac gccgggaaga gcattcagct cctgctcgat ctccttcgga tagacgttga
     3061 agccgccgga tatgatgaga tccttggcgc ggccgacgat ggtcacgcga ccatcctcgg
     3121 acatcgtcgc caggtcgccg gaaatgaaat agccgtccgg ccggaactct tccctggtct
     3181 tctccggatt gcgccagtag cccttgaaga cgttggggcc cttgacctca agcacgccga
     3241 cctcgcccgg cggcaccggc tctccttccg gggtggcgat cctgaccgag atgccgggaa
     3301 gcgcatagcc gaccgtgccg gcaatccgct cgccgccctt gtaagggttc gacgtgatca
     3361 tgccggcctc ggtcatgccg tagcgctcga gaatcttgtg tccggtacgg gcctcgaact
     3421 cctggtgggt ctcggcgagc agcggcgccg acccggagat gatgagccgc atccgggcgc
     3481 aatgctctct gccgaacgac ggattgccga gcaagcgcgt gtagaaggtc ggcacgccca
     3541 tcatcaccgt cgcctcgggc atcaggcgca tgacggactc cagctcgaat ttcggcagcc
     3601 agatcacgcg cgacgaatta aggaacgccg tgttgagcgc gacgaagagc ccgtgcgtgt
     3661 ggaagatcgg cagcgcgtgc agcaggacat cgccgggctc gaatccccaa taggagtgca
     3721 gcacctcggc attggacgac aggttgcgat gcgtaatcat ggcgcccttc gagcgccctg
     3781 tcgtgccgga ggtatagaga agagcggcca aatcctctga atcgcgcgcg acggtctcgt
     3841 gctcggcgct ctgctgccgg gcaaggtcgc tcaacgagcc gctgccatcg gcctcgagtg
     3901 ttaccacctt ggcggttttc aatgcggcgg cgatcgactc cacgcgcggc agcttctcgt
     3961 tcggcacgac gatcagagtg ggctccgcat cattgatgaa gtaatcgagc tcggcggccg
     4021 tgtaggcggt gttgagcggg ttgaaaacgg cgccgatctt tagcgtcgcc aggtaaaggt
     4081 gcacgttcgc gagcgatttc tccacctgcg ccagcacacg gtcacccggc ttcacgccca
     4141 gcgacaccag cgcgttcgcc atgcgcgcaa cgtcatctgc gaggctggcg tatgaaacgt
     4201 cggcgccgcc cggaatggaa aacgctattg ccgaacctgc ggcgcggaac cgcgcttcga
     4261 gtgccgcgta gagatttcct tcgatcgcca tcggtttctg ccatggattt aggtgtgcgc
     4321 cggccctgcc cggctgaagg cagggggata aaacgtcttt tggccgcgat aggcaagtcc
     4381 tggctggacg actgcgtcac gcagagcgcg cctcactgag gcgatgattg gccagagcag
     4441 gatcagctct ggcctccagc ggcccgttct gacagctcct cggtcaccat gtcagcggag
     4501 tacgctgctg ccgcagtgcc ttcggcacga ggatcgagct cgaagaccgt cggggtggtt
     4561 ttcggcaccg cgacaaagcc ttccttgtgc tccgccggca gcttcggacg cagcttcacg
     4621 cggatcagca tgacgagtgc gatgagcaaa tgcgcggccg cggtatgcag gaagagtgct
     4681 ccgtgcccac cctcgctcat gacaaagccc gccatcagcg gaccgagtac ggcgccgatg
     4741 gagaaggtca gcagcagccc gctcgacacc tctacggcgc gcttcttgtg aacgaggtca
     4801 ttggcatgcg caacgcacag cgtgtaaatg ggaaaagcgc agcaaccgta gagaagtgcc
     4861 atcgggaaaa tggccatggg gctgctggtg ctcaatatgt aaaggccggc gccggcaaag
     4921 gctccagtgg tgccgagcgt cacgagcaac ggccgccggc cggcacggtc cgacagatag
     4981 ccgatgggcc attggctggc cgcgccgccg agaacagcca ccgtcatgaa catcgcgact
     5041 tccgctagcg agaagccggc gctgcggcca tagagcggcg cgagcgccca gaacgcactg
     5101 ttcgcaacgc ctgtgaagaa gcagccggcc accgcagcgg gcgaaatgtt gaacaaccag
     5161 acaggtcgca cccgggcggt tttcggcgga atcggcgccg atgtcttggt gagtgcgacg
     5221 ggaactgccg ccagcgaata aaggatggcc acgagcgaga acagctcgaa cccgcccggg
     5281 cttccaatcg ccatcatctg catgccgacg gtgacgacgg tcaggttcac gaccgtgtag
     5341 gacgcgagca cgcgtccccg cgtctccatg gtcgatgcgc cgcagagcca gctttctatc
     5401 cccatgaaaa gcccggcaaa gcaaaggccg ttgagggccc gcagcagcga ccaggcgatg
     5461 ggctctggcc agatcgcgtg cagcagtggc gtgatggtaa cgatggcggt gaaagcgacg
     5521 aacccgcgga tatggcccac gcgcgcaatc actttcggcc cgagcagaca acccgcgacg
     5581 agacctgcgt aatagaacga gccgagcagg ccgatctcga gccgtgaaaa ctgttcgaca
     5641 tcagcgcgca ccggaatcag cacgccgagc aagccatttc ccatcaaaag gatcgcggcg
     5701 gcaatcagca gcgcggtgac gggtctgagg aggccggcca tggggcttcg ctggtgtgcg
     5761 cggatccgcg cggttgaggc aaattgatca gcattttgcc atacgagtgg catcggccag
     5821 tattttggcg tgatcgaagg ccagcggcaa tctgcgccag tccgcgaccc aacgggctgc
     5881 cgccgcgtcg tcgcctgcct gaactttgct cgacctcaca cgtgtcaaaa atacgacgga
     5941 cgcggtatgc ccacgcgggt cgcgcttcgg gtccgagtag accccgacga gcgtcagccg
     6001 gccggctttc acgcccgtct cctcaatcag ctcgcgccgg caggcatctt cgaccgtctc
     6061 gcccacatcc acgaagccgc cgggcaacgc aagatgcccc ttgaacggtt cattcttgcg
     6121 ctcgatgagc agcacctcgc cacgcgggtt gaatacgacg caatcgaccg tcagcagggg
     6181 tgtcttgggg ggaggcatgg tttctaaatt tctgaaatga atgatgtcgg gcaggtccat
     6241 ccctcccatc ttgtgggagg aatggaggaa tatggagcgc tattccgcgg ccagtttcac
     6301 tggcgccgaa ttactgaatg cctgcagtag gtctgcctcg cgggcttttg cggccttgta
     6361 gttcgcaagc ttcacgtgac cgaagccctt gatttccagc ggcagctcgg cgagcgcaac
     6421 ggcgatgccg tggttgccct ggttgaggtt cgcggccagg tcatccagaa gacgctcgta
     6481 atcggcgatc atttgacgtt ccagcttgcg ctcggccgaa tagccgaaca cgtcccaccg
     6541 cgtaccgcgc agcttcttgc ccttggcgag caggcggaaa accgggagca tccagccgcc
     6601 taagcggatc ttgcgcggat ggccggtcgt cttgtccttg aaaaatccgg aaaggatcgg
     6661 gggcgcgaga tagaattcca tcgcccggac gccctcgaat tgctcattga cctgccgctg
     6721 gaaggctccg tccgtgaaga gccgagcaac ttcgtactca tccttatagg ccagcagctt
     6781 gtggtaggca cgggcaaccg cggtggcgag acctgaccgg ccgggtgcct tctcgttttc
     6841 gaccccggca atccaggcga cctttgcccg atagcgggat gccagctctt cgtcctgata
     6901 ggccgcgagc tggcgagcgc ggaacgcgac gaggccgtcg agtgtctccg gctcgctggc
     6961 atgctggtcg gccggcttgg ctacacggtc taccgcggct ttgtcatggg ccgcgagacg
     7021 gccaagccgg aaggcggagc ggttcatgtc gacggacgcg ccgttgagca cgatcgcctg
     7081 ctcgatggcg gccgcaccga tcggcacgta gccgagctga taagcgtagc cgagcaggaa
     7141 aatattggac gctatcgagt cgccgaacag cttcaccgca tagtcatggg cgtcgaacgt
     7201 gtgcagcggt cctttgcgta cgcggtcggc gatggcttgc agcaattggt ctccgggcac
     7261 ggtcagcttc ggattgcgcg tgaaatcacc cgtgatcatc tcgtgggtgg atacgacgac
     7321 ggcggtctca tctggccgga tggtttcgag gatcttgttc gatgccgtga cgacgagatc
     7381 gccgccgagc acgaggtcgg cattggcgac gccgatgcga atggcgtgaa tctcgtcagc
     7441 cgtgcgcgcc acgcgcatgt gacaggccac cgcgccgccc ttctgcgcga gacccgtcat
     7501 gtcgatgctg ccgaacccct tgccttcgat gtgggcagcc tggccgagaa cggcgctgac
     7561 ggtgacgaca cccgtgccac cgacgcccgt gacgagcatg gaataaggcc cgtttaggct
     7621 tggggccttc ggctcgggca gggaggctac aagctcgtca agccctccgt tgccggaggc
     7681 ggctcccttt gatttcctga ctttggcgcc gtgcaacatg acgaagctcg ggcagaagcc
     7741 ctcgatgcag gagaagtcct tgttgcacgc ggactgatcg atggcgcgct tacggccgag
     7801 ctccgtttcg agcgggacga cggccacgca attcgatttc tttccgcaat cgccgcagcc
     7861 ctcgcagacc tcggggttga tgaagacgcg cttgtcggga tcgggaaact cgccgcgctt
     7921 gcggcggcgc cgcttctccg cagcgcaggt ctgatcgtag atgagagccg tgacgccttc
     7981 cgtctcggcc agcgaccgct gcacggccat caactcgcgg cgatgatgcg ccgtaacgcc
     8041 gttgggcaga ctgtaggcgc ccttgtactt atccggctcg tccgagacca cctcgacgcg
     8101 gcggacgccc tctgccagaa gctgttgcgc aatctgcggg acgccgattc ctccgtcgag
     8161 cttctgcccg cccgtcatgg cgacggcgtc gttgtagaga attttgaaag tgatgttcgt
     8221 accggccgcc atggccgctc ggatggcaag gctgccggag tgcagatacg tgccgtcgcc
     8281 gagattctga aagacgtgct tgcgcttcga gaacggcgcc tcgccgatcc agttggcgcc
     8341 ctcgccgccc atctgcgtga agccctcggt cgagcggtcc atccactggg ccatgtaatg
     8401 acagccgata ccggcgtaag cccgcatgcc ctccggcacc accgtcgagg aattgtgcgg
     8461 acagccggcg cagaagtacg gtgtacgcag aaatgcttcc ggctgagacg gccgattgcc
     8521 cttgagctgc ttcagctcgg caactttcgc cttcagagct tcgccgccga gagtccaccg
     8581 gatcagccgc tcgccgatcg caatggcgat gtcggtcggt tcgagagcgc ccttggcggg
     8641 gaacagcggt tgcccgttct cgtcattctt gccgatcacg cgcggatggt tcggcgcgtc
     8701 gaagagctgc tccttcacct gcgtctcaat cagcgaacgc ttctcctcga ccaccatgat
     8761 cgtgtcgagg ccctccgcga agctggcgat gccgcgcggc tccagcggcc agaccacgcc
     8821 gaccttgtag atgcggatgc cgagctcgtt cgccttcacc tcgtcgatgc cgagctcatc
     8881 gagggcctga cgcacatcga gatagctctt gcctgtcgtg atgatcccga gcttgggcgc
     8941 cggcccgccg ctcatgacga ggcgatcgag cttgttggcg cgactgaacg cgagaatggc
     9001 gggcagcttg aaatcatgca gccgcgcttc cttggccagc ggctggtcat tgagccggat
     9061 gttgaggccg tccggcggca tccggaactc gtcttcgctg gggatgacca ccttcacacg
     9121 atcgatccgg ccgtcgaccg tcgccgtgct ttcgatgttg tccttcacgc acttgagcgc
     9181 gacccacacg ccggcgtagc gcgacagggc gaagccgagc agaccgaagt cgaggatttc
     9241 ctggacgcct gccgggttaa gcaccgggat catggcatcc atcatcgcga attccgactg
     9301 gtgcgcggag gtggagcttt cgcaggtgtg gtcgtcgccg agaagcgcca gcacgccgcc
     9361 atacttcgag gttccggcat gattggcgtg gcggaacacg tcgccagtgc ggtcgacacc
     9421 aggtcccttt ccgtaccaga tgccgaaaac gccatcgtac ttgccctcgc ccctgagctc
     9481 agcctgctgt gagccccaga gagcggtggc ggccaggtcc tcgttaaggc ccggatggaa
     9541 atggatgttg tgcgggtcga ggatggatcg cgcgcgcaga aacgcgcctt cgagcgacgc
     9601 gatcggcgag ccgcggtagc ccgtgacgta gccggcagta ttcagtcccg cttgccggtc
     9661 gcggacctgc tgcatcagcg cgaggcggac gatggcctga gttcccgtca ggatctgacg
     9721 cgtttcgctg agatcgaact tgtcgctcag actgactgcg cgcttcggca ttgggcacct
     9781 cggaattggg cgtcgacgca gcgttcgcga gtcgagccac ccccgactag gtcagtattg
     9841 ctgacatatt cattacgcga gtaacgccga acgagcaaac gccagcagat cgccagcgta
     9901 cgatctctag aatgttcgtt gcaacggcct gggtgcaacc gaccggctgt gcactgcaac
     9961 agagtcaggg cctgccggtc agccgcccgg gtccagaata ttcaaactag ttgcggcgag
    10021 tgaacttgcc tgtggtattg gacatagctg tcccgaatgc gcgctgaatc tggtgaggtt
    10081 acgccgattt cctcgatgag tgcggctgtg gcctgatcga tcgccgtggc gagcattgga
    10141 ggaaattcca cgacctccag atctggcatg gctgtgatga cctgctctgc aaggcaagca
    10201 tggctgagcg attcggccga tgatacggcc agctcatgcg cggcgagagc ttcgagcgcg
    10261 agccgggtag attggtccag gcgctcccaa atcgccatcc gcacatgcat ggcgatcgcc
    10321 atgccatttc gatggatacc gccgatgtgg aaatgcgtgg ccacttgggg caaaccgagc
    10381 atcaggcctg cgagcgggtt gccccattcc actgcggtga gctcacgatt cgccagaccg
    10441 tcacgcagct gagatgcggg ccattcgcac ggaatggctc caagtttgct ggcaactgac
    10501 gcgccaggtc cgcctaccgc caaagacacg ccggccaaat ccgcggcact ccggatcggg
    10561 caagcggtcc acagtccggg gctctggccc gtatgccccg ccagaagggg cttcaatcca
    10621 tggcgcgccg caagatcgtc ccaaagcatc tgtccgccgc ccgcgacgag ccacgcctgc
    10681 aaatgggcca aatcgagccc gtacgatccg ggcagaccgc agaagaaggc aaaggccggg
    10741 tcctgctcga ccgagaccgg gccgaagacg agatctgctt cttctgaagc ctcgaccgcc
    10801 accagatggc atctgccatc gagcaactgg cccagccgag tgagcatccg ctgagcggca
    10861 tcgccgaaaa tcggcacgtc gggttcccaa gcggccgcga atgtcagctc gatggtgccg
    10921 cgggaaagag ccggtgcagc taaagggtcg ggatcagctc ccgcaagcac cggcgtggat
    10981 gacagggcga ggcccgaccc ggctgccacc gtcgttgatt tcagaaatgt cctgcggtcc
    11041 atgccaaccc ctccccgcat cgcgtgtccg atgcttccct gccgggcatc aaacccgaac
    11101 agaagatctc tgacaatgcc ctggtgagca gtgaagatca gttggcggcg agcattcggc
    11161 cgatgatctg ctgaaacgag gcctgaccgg tgtacccggt gatccgacca gcctcccggc
    11221 catcgatgag caggacggcc gtcggcacga tcgtcagcgg acccgaaaga gcggactcca
    11281 gcggacccgc atcattcaca tcgacaaatc ggatcggcag ctcgctggat cggccggaat
    11341 tccggtattc cggcagaacg tcacggcgga acaggcggca atagacgcaa tcggcggttt
    11401 cgaagacgac cagctcgcgg ctgacctgcc cgcgccgcat atccttcttc gaagcgggcg
    11461 ccgtcagggc gccaagcgta gctccggcca ccactgccgc gagcgtcagc ataatgaact
    11521 ggctacgcga cacgcccccc tcccagcgga acagccttgt gatctgccct gcgcaacccg
    11581 ccgatgttta accagtcgtt aacggatcgc cagaacagaa atgaacgtgt ggttaaggac
    11641 ggtgtgcgaa acgaatgagg acgccccagc catccgagcg gcgtcgccca tttcgggcca
    11701 cacattcagc ttctcgattg tcgcttggcc atggtgtacc acttgaggtc agcgaccgcg
    11761 taagaagggc ggcgcagcgc aattgggaac gcgatcagga gatgggaggc accgagccat
    11821 gtcgattagc cgccgtacgt tcatcgcgac tgccggagcg ggcatcgccg cccctgccgt
    11881 tctccgagtg acaagggcaa atgccgccga ggtgacgctg aggctgcatc atttcctgcc
    11941 gcccgtcgcc agcgttccga agcacatgct ggagccatgg gccaagcgca tcgaaagcgg
    12001 gacaggcggc cggttgaaga tcgagcttta tccctccatg caactcggtg ggaagccgcc
    12061 gcagctctac gatcaggcga aggacggggt ggcggacatc gtctggaccc tgccgggcta
    12121 caccgcgggc cggtttccct ccacggaggt gttcgagctg ccgttcgtgg cggcgcggac
    12181 ggcgcgcgtc aatgcacgcg ccgctcagga gtttgccgac cagcacctga aagaagagac
    12241 aaaagatgtc aagctgctct gcttctggtg ccacgaccat gggctcattc atgcgaagcg
    12301 gcaggtcaag accatggagg acctgaaagg cctcaagctg cgcttcccca cgcggcttgc
    12361 cggcgaggct ttggaagcac tgggcgcaag ccccatcggc atgcccgtgc c
//
LOCUS       DASVDU010000327         7743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_590478, whole genome shotgun sequence.
ACCESSION   DASVDU010000327 DASVDU010000000
VERSION     DASVDU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590478"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..1726
                     /locus_tag="VF226_21810"
     CDS             533..1726
                     /locus_tag="VF226_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX7076686.1"
                     /translation="MAPISASPALSHLRVLDLTRVRAGPACCRVLADFGADVIKIEAP
                     SGVDPNEGISGPRHGYDMLNLHRNKRSLTLNLKTPTGLALFKRMVKKADVVVENYRPD
                     VKHRLGVSYEELRQVNPRIILASISGFGQDGPYSKLPGFDQIAQGMGGLMWVTGLPGQ
                     GPVRAGAAVADISAGLYAAIGILIALAERERSGVGQWVHTSLLQAQIALLDFQAARYL
                     VDKVVPEQAGNDHPYSTPMGVFQTRDGYINLGVGGDGQWRSLCEVLGRAELGTDERFA
                     TVEARYRNRPQVRSLIEPLFLERTSAEWLRALAEAGVPAGPIYKMDEVFADPQVQHLK
                     MAVPCAHPARGDVELVGQPLVLSRTPAQIKEPAPDKGEHTDEILAEFGLSAAEIERLR
                     SERVI"
     gene            complement(1781..3211)
                     /locus_tag="VF226_21815"
     CDS             complement(1781..3211)
                     /locus_tag="VF226_21815"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX7076687.1"
                     /translation="MSISVSDNLGCVTERSSIEQSISSFIAKTRYEDLPAEAIKKTKT
                     FLLDSIGVGLAGTSDPNAARVLAAAERWGTGDEATAWGSGAKLPAPSAAFANAYLIHC
                     LEYDCIHERAVMHPMATLLPALMAWCERAAQQGKPANGKRFLNAIAVGVDISGLIGSA
                     TKSGLRFFRPATAGGFGAAAAIASVAGWGDDQVRNTLAIHCGQVSGTMQAHMEGSPTL
                     GLQIGFNSRAAVVANDLAAAGLTGPRDFLTGQFGFFSLFENGSFDPKVIENELGRVWQ
                     ITQLSHKPFPSGRLSHGGVDGLRRLMTEHGFPAEAVAEVTVHVPPLVMRLMGRPLAEN
                     PTANHAKLCLPFVMGTYLARGRVDVPDFGSAEMLNDPAVHAYGSRIRLVPDDNTDQNA
                     IAPQRVVVRLTDGTEHATTITAIYGHPDAALTDAENLDKFSRNCSYARPPLSPAMQDR
                     TIAFIEKLEEAEDVSQLPRLFVPPRA"
     gene            complement(3449..4765)
                     /locus_tag="VF226_21820"
     CDS             complement(3449..4765)
                     /locus_tag="VF226_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7076688.1"
                     /translation="MANKRTDTAETSSRAVTRRSFLETALAGGALAGTAYISLARDMS
                     AFAQASGPIVIGHHCELTGGFASWGYWHNKCALAAAKYINESGGIAGRKLELVTEDTE
                     SNPATGARKLRSLIQRNGASFVVGSVHSGVMLASIPVATELKTVYFSQGEATEATGEK
                     GTRYSFRTGTDTYGLSAAGAPWAVENLGKRWTIISPDYAWGHSHYAEHKAVVEKLGGT
                     VSDPIFVPLDAKDLVPYLTRIPEDTEVLFSVFFGALSVAFYTQAKNMGLEKTMRMYSV
                     AGTIEAIAPADLNGAAEGVYIVENFPRMAKYKDDEFHREYLKLIGTDDIHAREVGSDR
                     VNAKSHSWQSWEDIFALKQAIEMSGWKSKKDDQGVIEALEGLEMPNSLGFPQGAKTLR
                     KEDHSGIIDCYISRIENNEFHVKKKVTREEMAKLMPPRFDLSKQPL"
     gene            complement(4785..5483)
                     /locus_tag="VF226_21825"
     CDS             complement(4785..5483)
                     /locus_tag="VF226_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076689.1"
                     /translation="MLSVSGLTAEIGRVPVLRGVTLSVEPGETVALLGRNGVGKTSTL
                     RAILGLLRRTGGTVTYKGQDLSKVPAHRLASLGLGYVPQGRGIFPLLSVEENLCLGLT
                     RRPSKELLDELFHRFPRLEERRAQAAGTLSGGEQQMLALARCLIMQPSLIMLDEPTEG
                     IMPKLVAQIRSEIAEISRRGIAVLIVEQNVRTAMKLANRVYLMERGQVVAEATPEKLK
                     SSPELVHRYLGVSL"
     gene            complement(5490..6251)
                     /locus_tag="VF226_21830"
     CDS             complement(5490..6251)
                     /locus_tag="VF226_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7076690.1"
                     /translation="MRAGASALEVSGLTKRFGGLTAVDNVTMKLASGKLNAIIGPNGA
                     GKTTFFNLVSGELRADSGSVRVHGTDLTGLPPHRIVQAGVSRTLQIKSVFSNLSVADN
                     VRAAVMAHEKIRSPFRPLLSFSAVNERVDELIADVGLSEYRDRTASTLSYGDIALLEL
                     ALALANKPRLLLLDEPVCGMGPEETERTTRIIRNIAQSTDVIIIEHDMEVVFSIADHI
                     VVMAQGAVLAEGDPQAISNDARVQEAYLGSPEDEE"
     gene            complement(6251..7279)
                     /locus_tag="VF226_21835"
     CDS             complement(6251..7279)
                     /locus_tag="VF226_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076691.1"
                     /translation="MTRKLNTVALVLLAAGLISVPFVLPAFGASFWVNIIAEILIWSL
                     LASSVNLLLGYVGLLSFGQALFFGFGMYGATLSVLHLGAGFWTGMTAGVLAATLMALI
                     AGVLAVRLTWHYFAIITIVFSLIFYFAAMSAKPLTGGDDGLSFTAPTVFDFGGTTLTL
                     ADPTAQYMIILAIVAGCYVLKGLLVNSPLGLAFAAVRENDRRAALIGINVYRVRLTAF
                     VLAGALAGISGALFAFYGRYASASYMFYHVSGEAVVWAIVGGTGSLLGPLFGTGALIA
                     FRELVSTAWENYLLAVGAITILVVMFAPRGLAGLWNDLVTGLSGYKAAIPEPKAAAVE
                     AEKKVGAA"
     gene            complement(7276..>7743)
                     /locus_tag="VF226_21840"
     CDS             complement(7276..>7743)
                     /locus_tag="VF226_21840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX7076692.1"
                     /translation="GIDYDIYRVVAAVFAAVCLAGFFLFLKHSKIGTWIRAVRHDPET
                     ATALGVPVVRICAITFGLGTAIAFLGGAIAAPITTVEFRTGVDILPFCFMAVIIGGLG
                     NLEGTVAAAVMLAVLEGVVTSVTDPTIARIFSLAFMSLVLLLRPHGLFTGAKR"
BASE COUNT         1573 a         2350 c         2330 g         1490 t
ORIGIN      
        1 cccttcaccc tccgactggg aaagttgccc gaaatgctcg agtcgcctca gtgtcgacgg
       61 tgagcgtgtc agagtccatg accacaccat agtcgcggcg tgcacctcgc atcagaacgg
      121 ccggaacgcc atcgagcacc acgcgcgacg tgtccgccgg gtcgagggaa cctcgagaat
      181 ggtcttcctg cccgtgcgcg cgtccgctgc cgcgagatcg gtaaaagtgc tgccggtgtc
      241 ctcgccgacc acgactccca tggcgatgct ctccttgctg gcgggcataa ttttgtttga
      301 tggctgctgc caaccgtgtc gcagctccac ctcccacgac attcgatcaa atttgcccgt
      361 gcggcaagta tgtcatatta tccgtacata tctgttgctg gcagatacct cgataatcga
      421 gaggcaaaac gagatgcgca gcgacggaga ttcgacgctc gatattcgca caactccgca
      481 gctagccatc acgcagtggg gacgacaatc tgaacgccag ggagtgcgtg atatggcacc
      541 gatttctgct tcccccgctc tctctcatct gcgcgttttg gacctgacgc gcgtgcgcgc
      601 tggtccggca tgctgcaggg ttctcgccga cttcggggcg gacgtcatca agatcgaggc
      661 gccgagcggt gtggatccga atgaggggat cagcggacct cggcatggct atgacatgct
      721 caatctccac cgcaacaaga ggtcgctaac gctgaatctg aagacgccga cgggactagc
      781 tctgttcaag cggatggtga aaaaggccga tgtcgtggtc gaaaactatc gccctgacgt
      841 aaagcatcgg ctcggtgtca gttacgaaga gctccgccag gtcaacccgc gtatcattct
      901 ggcctcgatc tccggcttcg gacaggacgg tccttacagc aaacttcccg gcttcgatca
      961 aattgcgcag ggcatgggtg gattgatgtg ggtcacggga ttgcccggtc agggtcctgt
     1021 ccgtgccgga gcagcagttg ccgatatttc cgcaggactt tatgctgcca tcggcattct
     1081 gatcgccttg gctgaaaggg agcgttctgg ggtcggacag tgggtccaca catcgctcct
     1141 gcaggcgcag atcgccctcc tcgatttcca ggcggcccgg tacctcgtcg acaaagtcgt
     1201 ccccgagcag gccggcaatg atcacccgta ctccacgccg atgggagttt ttcaaacgcg
     1261 cgacggttac atcaatctcg gcgttggcgg agatggccag tggcgcagcc tatgcgaggt
     1321 gctggggcgc gccgagctcg gcaccgacga gcgttttgcg acggtcgagg cacgctaccg
     1381 aaatagaccg caggtgcgct ctctgatcga gccgctgttc ctggagagga caagtgcgga
     1441 gtggctgagg gcgctggctg aggcaggtgt ccccgcagga ccgatttata agatggacga
     1501 agttttcgct gatccgcagg tccagcacct aaaaatggcc gtgccgtgcg cacatccggc
     1561 tcgcggcgat gtcgaacttg taggtcagcc tctcgtcctt tcacgcactc cggcacagat
     1621 caaagagccc gcgccggata aaggtgagca taccgacgaa atcctcgcag agtttgggct
     1681 gagtgccgcg gaaatcgaga gattgcggtc tgagcgggtc atctaagctg aaccgcgcag
     1741 aaaatttcgc cttcctctca tatgagtaaa tcaccccgct ctatgcgcga ggtggaacga
     1801 acaggcgcgg gagctgtgaa acgtcctccg cctcttccaa tttctcgatg aaagcgatcg
     1861 tccggtcctg cattgcggga gagagtggcg gccgtgcata gctgcaattg cgcgagaact
     1921 tgtccaggtt ctcggcgtcc gtcaacgcgg catccggatg accgtagatt gctgtgatcg
     1981 tggtcgcgtg ctcggtgccg tcagtgagcc ttacgacgac gcgctgtggc gctatcgcgt
     2041 tctgatcggt attgtcatca gggacgaggc gaattctgga tccatacgcg tgcacggctg
     2101 gatcattgag catctcggcc gagccgaaat ccggcacgtc gacgcgtccc cgtgccaagt
     2161 aagtgcccat gacgaaagga agacacagct tggcgtgatt ggctgtgggg ttctcggcga
     2221 gcggacgacc catcagcctc atcaccagag gcggcacatg gaccgtaacc tcggcgaccg
     2281 cttccgctgg gaaaccgtgc tctgtcatca accggcgcaa tccatcgacg ccgccgtggc
     2341 tcagccggcc actcggaaag ggcttgtgcg atagctgtgt aatctgccaa acgcgcccga
     2401 gctcgttctc gatgaccttc gggtcgaatg acccattctc gaacagcgaa aaaaatccga
     2461 actggccggt caggaagtct cgcggaccag tgagcccggc ggcggcaaga tcgttcgcca
     2521 ctacggcagc acgggagttg aaaccgatct ggagaccaag cgtcggcgag ccttccatgt
     2581 gggcctgcat ggtgccggaa acctggccgc aatgaatagc aagcgtgttt ctgacctgat
     2641 cgtctcccca gccggcaaca cttgcaatgg cagccgctgc accgaagcct ccggcagtcg
     2701 ccgggcggaa gaaccggaga ccggatttcg tcgccgatcc gatcagcccc gaaatgtcca
     2761 cccccacggc gatcgcgttc aggaaccgtt tgccattcgc cggcttgccc tgttgagcgg
     2821 cacgctcgca ccacgccatg agtgccggaa gcagagttgc catcgggtgc atcactgcgc
     2881 gttcgtggat gcagtcatat tcgaggcaat gtatcaggta ggcattggcg aaagccgcgg
     2941 atggcgccgg gagcttggcg ccgcttcccc atgctgtcgc ctcatcgccc gttccccaac
     3001 gctcggccgc agctaggacg cgagcagcgt tgggatccga agtcccagca agtcccacac
     3061 cgatcgagtc gagcagaaag gtctttgttt tctttattgc ttcagcaggc aggtcctcat
     3121 aacgcgtttt ggcgatgaat gacgagatgg actgctcaat gcttgaccgt tctgttacgc
     3181 aaccgaggtt gtccgacacg gagatgctca cacgcttcga tcattagggc tcatcgacgc
     3241 cgaaccctac ggccgtagac tcggcggcgc aagcgcgcgg tgcgtacgcg atccgctggc
     3301 actggaccaa ggatcacgcc agcctagcta caactctgcc acgcggcagt tcgagagttg
     3361 agcaggggtt gaactacctc agcgggcttt cgacgcggcg gtgcaccggg tcgtgcaccg
     3421 ctacgatttt ctacagtcgc aaaagaaatc aaagtggttg ctttgagagg tcaaaacgag
     3481 gcggcatcaa ctttgccatt tcctcgcgtg ttaccttttt ctttacgtgg aactcattgt
     3541 tctcgatccg gctgatgtag caatcgatga tgccgctatg gtcttccttc cgcagcgttt
     3601 tggccccttg tgggaatccg agagagttcg gcatttcgag gccctcgagg gcttcgatca
     3661 cgccctggtc atccttcttg ctcttccagc cggacatctc gatggcctgc ttcaacgcaa
     3721 agatatcctc ccagctctgc cacgaatgac tcttcgcatt cacgcgatcg gagcccacct
     3781 cgcgagcatg gatgtcgtcg gtgccgatga gcttgaggta ctcgcggtga aactcgtcgt
     3841 ctttatactt cgccattcgc gggaaattct cgacgatgta gacgccttct gcggccccgt
     3901 tcaggtcggc cggtgcgatt gcctcgatgg tgccggccac ggagtacatc cgcatcgtct
     3961 tttccaggcc catattcttg gcctgcgtgt agaacgccac cgacagggcg ccgaagaaca
     4021 cggagaaaag cacctcggtg tcttccggaa tccgggtgag gtatggaacc aggtccttgg
     4081 cgtcgagagg aacaaaaata ggatctgaaa ccgtgccgcc cagtttttcg accacagcct
     4141 tgtgctcggc gtaatgacta tgtccccatg cgtagtccgg cgaaatgata gtccaccgct
     4201 ttcctagatt ctccactgcc cacggcgcgc cggcggcgga aagcccgtag gtgtccgtac
     4261 cagtacggaa gctgtatcgc gtgccctttt cacccgtcgc ctcggtcgct tcaccttgtg
     4321 agaagtagac ggtcttcagc tcagtcgcga ccgggattga cgccagcatg acacctgaat
     4381 ggactgagcc tacgacgaac gaggcgccgt tgcgctggat caacgatctc agctttcgcg
     4441 ctccggtggc ggggttggat tcggtatcct cggtcaccag ctcgagcttg cggcccgcga
     4501 taccaccgct ctcgttgatg tacttcgctg ccgcgagggc acacttgttg tgccagtagc
     4561 cccaggacgc gaagccgccg gtcagctcgc aatgatgccc gatgacgatc ggcccgctcg
     4621 cttgagcgaa ggcggacata tcgcgagcga gactgatata ggccgttcca gccagcgctc
     4681 cccccgccag cgccgtctcg aggaagctgc ggcgcgtgac agcccgcgag gatgtctcag
     4741 ccgtatcggt acgcttgttt gccatatcgc tgtcctttcg gtgactaaag agagacgccc
     4801 aggtagcggt gaacgagttc gggggatgat ttgagcttct caggcgtggc ctcggcgacg
     4861 acttggccgc gctccatcag gtagacgcga ttggcgagtt tcatcgccgt ccggacgttc
     4921 tgctcgacga tcagcactgc gatgccgcgc cgcgagatct ccgcaatttc gctgcggatc
     4981 tgcgcaacca gcttgggcat gatgccctcg gtcggctcgt cgagcatgat aaggctcggc
     5041 tgcatgatga ggcagcgggc gagcgccagc atttgctgct cgcctcccga caaggtccca
     5101 gccgcctgcg cacgtcgctc ctccaggcgc ggaaagcgat ggaaaagctc gtcgagcaac
     5161 tccttcgacg gcctgcgagt gagcccgagg cataggtttt cctcgacgct cagcaatgga
     5221 aagatgcctc gcccttgcgg aacatagccg agaccgaggg aggccagccg atgcgccggc
     5281 accttggaca ggtcttggcc cttgtaggtg accgtcccgc cggttctgcg cagcaggccc
     5341 agaatggcgc gtagagtcga cgtcttaccc accccgttcc ggccgaggag cgcgactgtc
     5401 tcgccaggct cgacgctgag tgtaacgcct cggaggactg ggacgcggcc gatttcagcg
     5461 gttaggcctg agacagacag catcgatgct tactcctcgt cctcaggcga accgagatag
     5521 gcttcttgca cgcgagcatc gttactgatc gcctgcggat ctccttcagc caggacagct
     5581 ccctgtgcca tgaccacgat gtgatcggcg atcgagaaga cgacctccat gtcgtgctcg
     5641 ataatgatga cgtccgtgct ttgggcgatg ttacgaatga tgcgtgtcgt tcgctctgtc
     5701 tcctcagggc ccatgccgca caccggctca tcgagcagca gcagccgcgg cttgttcgcc
     5761 agcgccagcg caagctccag gagcgcaata tcgccgtagg aaagagttga ggccgtgcgg
     5821 tcgcgatact cggacagtcc gacgtccgcg atcagctcgt cgacgcgctc attgacagcc
     5881 gagaaggaga gtaggggccg gaacggtgaa cggatcttct cgtgcgccat cactgccgcg
     5941 cgcacgttgt cggcaacgga caaattgctg aaaacgcttt tgatctggag agtgcgagat
     6001 actccggctt ggacgatgcg gtgcggcgga agacccgtca gatccgtgcc gtgcacgcgg
     6061 accgagccgg agtccgcccg cagctcgccg ctcaccaggt tgaaaaacgt cgtcttgccg
     6121 gcgccgttcg gcccgatgat ggcgttgagc ttgccgctcg ccagcttcat cgtcacgttg
     6181 tcgacggcgg tcagcccgcc gaaacgcttc gtcagcccgg agacctcgag tgcgctggct
     6241 ccggcacgca tcaggcagcc cccactttct tctccgcctc gacagcggcg gctttcggct
     6301 cgggaatggc ggccttgtaa ccggaaaggc ctgtgacgag gtcattccag aggcctgcaa
     6361 ggccacgggg agcgaacatg acgaccagga tcgtgatggc gccgacagcg agcaggtaat
     6421 tctcccacgc cgtcgagacc agctcgcgga aggcgatcag tgctccggtg ccgaacagcg
     6481 ggccgaggag tgagccggtt cccccgacga ttgcccagac aactgcttct cctgagacat
     6541 gatagaacat atacgaagca gaggcgtatc gcccatagaa ggcgaacagt gctcccgaaa
     6601 ttccagcaag ggctccggcc agaacgaatg ccgtcaaacg cacccgatag acatttatgc
     6661 cgatgagcgc agcacggcgg tcattctcac gcacggctgc gaacgccagc ccgagcggac
     6721 tgtttacgag cagtcccttc agaacatagc atccggcgac gatggccaga atgatcatat
     6781 actgggctgt cgggtcagcg agggtcagag ttgtcccgcc gaaatcgaac accgtcggag
     6841 cagtgaatga caggccgtca tcccctccgg tcagcggctt cgcgctcatc gccgcaaaat
     6901 aaaaaatgag agaaaaaaca atagtgatga tcgcgaaata gtgccaagtc aggcggactg
     6961 ccagtacgcc cgcaatcagg gccatcaggg ttgcggcgag cacgccggcg gtcatccctg
     7021 tccagaagcc tgcacccaga tgaaggactg agagcgtcgc tccgtacatt ccgaaaccaa
     7081 aaaagagagc ctgtccgaac gagaggagac cgacgtagcc gaggaggaga ttcacgctcg
     7141 aagccaggag cgaccagatc aggatctccg caatgatgtt cacccagaag ctcgcaccga
     7201 atgcgggcag aacgaacggg accgatatga gcccggcggc caggagcaca agcgcgacgg
     7261 tgttgagttt tctggtcacc gcttcgctcc tgtaaacaat ccgtgcgggc gaagaagaag
     7321 aacgaggctc atgaaggcca gcgagaaaat cctggcgatg gtcggatccg tcacgcttgt
     7381 cacgacgcct tccagaaccg cgagcatcac ggccgccgcc accgtaccct cgagattgcc
     7441 cagccctccg atgatcacgg ccatgaagca gaacggcagg atgtcgacgc cggtacggaa
     7501 ttcgacggtc gtgattggcg cggcaattgc cccgccgaga aatgcgatgg ccgtgcccag
     7561 cccgaaggtg atcgcgcaaa tcctgacaac cgggacccca agcgccgtgg cagtctcggg
     7621 gtcatgccgc acggccctga tccaggtgcc gattttcgag tgtttcagga acagaaagaa
     7681 tcccgccagg cagactgccg cgaaaactgc tgcaacaacg cgataaatgt catagtcgat
     7741 gcc
//
LOCUS       DASVDU010000328        13317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_678465, whole genome shotgun sequence.
ACCESSION   DASVDU010000328 DASVDU010000000
VERSION     DASVDU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678465"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VF226_21845"
     CDS             complement(<1..467)
                     /locus_tag="VF226_21845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076693.1"
                     /translation="MKLSAHQVPYRAQAIAVLLLAALLLAPSPLIAAPAQAPGSRVVL
                     ELPDGFAASPDFSGFVGVANGASILLLEVPASAYPDMASGLSPEALSKKGVGQVQTGS
                     LDRQGEYVYVTGEQQTASGPYMKYILLFREPSATALVSINVPRSSFENGSAEPR"
     gene            complement(614..1627)
                     /locus_tag="VF226_21850"
     CDS             complement(614..1627)
                     /locus_tag="VF226_21850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7076694.1"
                     /translation="MRYRVLGRSGISVSELCLGTMNFGGPTNEPEARRIIDDALEHGV
                     NFIDTANVYTDGRSEEILGRALEGRRHRVILATKVAQPVDGKGPNERGLSRIHVMQAV
                     EASLKRLGTDVIDVYYTHRVDPKTPWEEVIRTFGDLIRQGKIRHWALSNVRGWQIAHV
                     CHLCRALNEPEPVALQPYYNLMNRQPEVEVLPAARAFSLGVVPYSPIARGVLSGKYRL
                     NATPEPGSRAARQDKRMMETEWRPESLAIAETLKAHAEARGLSLIAFAVAWVLNNQAI
                     SSVIAGPRTLEQWKPYLDALDVTWTPEDEAIVDRHVVTGHPSTPGYNDPGYPIEGRFP
                     AIK"
     gene            complement(1727..1951)
                     /locus_tag="VF226_21855"
     CDS             complement(1727..1951)
                     /locus_tag="VF226_21855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX7076695.1"
                     /translation="MADKTLDTKGLNCPLPILKTKKAIAEVPKGGTLEVLATDPGAVA
                     DFQAFCKSTGNEMVEHSEQGGVYRFVIRHL"
     gene            complement(2119..3075)
                     /locus_tag="VF226_21860"
     CDS             complement(2119..3075)
                     /locus_tag="VF226_21860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7076696.1"
                     /translation="MSQLRAAVVQASSVVFDWEATVAKAERLIAEAAAGGAKLLVFPE
                     AFVSGYPKGLDFGCRVGQRKPEGREDFRRYWESAIDVPGPATDRLGTAARENGVHLVI
                     GVIERDRGTLYCTVLFFSPNGDLLGKHRKLMPTASERLIWGFGDGSTLPVFDTPHGRL
                     GAVICWENYMPLLRTAMYSKGITLYCAPTADDRPTWVPTMQHIALEGRCFVLSACQMN
                     RRRDYPADYALAAEMDADQVVMRGGSCIIHPLGKVLAGPVFDEETIIYADLDLADVPR
                     SRLDFDAVGHYSRPDVFKLIVDERPQHPVSFIGEPNVPGDSA"
     gene            3549..5876
                     /locus_tag="VF226_21865"
     CDS             3549..5876
                     /locus_tag="VF226_21865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX7076697.1"
                     /translation="MPEHGTPKRSFALSFGLDRLGLVALKAPRVSALVILILSALAVV
                     GVMRLKVDDSLSELFRTDTPEFHQYEEIDQRFPSSEFDVLAVVEGPNLLARPQLEAFA
                     NAVIDLQLADGVDGLVSMLSARGKPDATGYAPPIVPDELPDGAAYDEMIKALRSNDIV
                     AGKFLSDDGQLALIVMSLNRQVVEERGAKAVIGNILETAEEALKGSGLTVKLTGAPVM
                     QLEIRNAVERDRLVYNGLGFLVGAGIAFLFFRRLSLTLIAVLGPSTAILWTLGVVGGL
                     DFRLNLFINVLTPLILVSGFSDSMHLVFSIRRDIIAGVDRVTAARNAVLDVAPACLLT
                     AMNACLALVSFSWAESALIRTFGKAALLAVGTSYIAVAVVVPTLAALLIRREKAPVET
                     LAERRGGGVNLLTRLSDWIVHRIAFRPIPYFLLSVAVVVASGLAYAQLEPHYRLADQV
                     PDKEHALAATDRLDQKLTGANPVHVMIKLKGGHSLYEPSTFEVISRAHTVLEKVAGLG
                     NVWSVDSLRRWLAEAGDDRIETVRSYIAVLPEHLVRRFIDKDEKAVLVTGRLPDVDAS
                     EILPVVEKIDQALEPVRQEFRDYEITVTGLPAIAARNSAKLIWELNIGLVGDMFVIFI
                     FLGIALRSVLSGVGSILPSLFPIFATGALLWATGEGLQFASIIAITVAFSLAIDSTIH
                     FLNRFRIEETRIGSGPGNATTALVAAMHHIGPVIVLTTIVLAMGLGVTVLSDLPSLRL
                     FGRLAAVCLFASLIGQLVILPATIALYRRYFPLKA"
     gene            5911..6384
                     /locus_tag="VF226_21870"
     CDS             5911..6384
                     /locus_tag="VF226_21870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7076698.1"
                     /translation="MPLLSSPLAIDEAWIDYNGHLNMAYYNVLFDRALDEVLLPTGLG
                     PDYVRETGCTYMAVEAHICYLREVFRADTVRVSVRVLDLDAKRMHLYCEMRHAIEDWL
                     AATSEWMFLHVDTKERRSAPWPPDIRERLDAIRRADEHLAPPARAGRRIGIPRKT"
     gene            complement(6635..7507)
                     /locus_tag="VF226_21875"
     CDS             complement(6635..7507)
                     /locus_tag="VF226_21875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7076699.1"
                     /translation="MTRTQPRVLQALAWMALALASFSSVAVAGREAARGATTLEMMFY
                     RCLISLIVILAIVLMGPGLKEMRTTRPTLHSVRALVHFVAQYSWLSALTMIPLAQLFA
                     LEFTAPIWVALLAPLLLGERLTQTRLSAAAVGFIGMTIVARPGAVALNAGVGLALIAA
                     IGFALSMLATKTLTRSESPLRILFYMFLIQAILSLFLLTGGIRLPDMATMGWICVLAL
                     AGLSAHYGLVRAFALADAIIVAPMDFLRLPLIAVVGALAYAEAFDPIVLLGGAVVIAG
                     NVLNLWGERRAATG"
     gene            7794..8405
                     /locus_tag="VF226_21880"
     CDS             7794..8405
                     /locus_tag="VF226_21880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7076700.1"
                     /translation="MTRITSVEALRAIYPPPSERALKKQLDKLDRHCRSFIGLSPFVV
                     ISSVGADGLADASPRGDRPGFVQILDYHTIAVPDWPGNNRLDTLSNIIVRPSVGLLFL
                     VPGVDETLRVNGEAELRDDEDLRSLFERSGKLPRLAILVHVKEAYLHCAKALMRSRLW
                     DPTSRIERSLLPTMNEMLQDQIGAAGPIEPQEDMIERYRKVLY"
     gene            8423..9457
                     /locus_tag="VF226_21885"
     CDS             8423..9457
                     /locus_tag="VF226_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEX7076701.1"
                     /translation="MRQQFASDNNAGMCPQALARFLEANAAGHAVGYGDDAWTQEARA
                     RIAALFETECDVFFVFNGTAANALTLAQLCRPYNAVIGHGFSHIETDEAGAPEFFTGG
                     AKLMTADTPDAKLTPEAVAALATRYSGPHHVKPAVLSLTQATELGTVYTPDELRALTD
                     VAKRHGLKVHMDGARFANAVARTGCAPADISWRAGIDVLCFGGVKNGLAVGDCIVFFD
                     RALSHEFAWRIKQAGQLNSKMRLVSAAWLGLLDGDVWLTNARHANAMADRLAAGLARI
                     PGVRFIAPVEANAVFADIPPDVQRALRDKGWQFYTFLGETGCRLMCAWDTEPATVDRF
                     LTDIAEAAGS"
     gene            complement(9467..10192)
                     /locus_tag="VF226_21890"
     CDS             complement(9467..10192)
                     /locus_tag="VF226_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076702.1"
                     /translation="MPVTYDFSGRVAVVTGGANGIGREIARRFRDAGARVAVWDLAAP
                     DLPGVDGHRVDITEPDAIAAALEATLSTHGGVDILVNNAGYVGPTVSVEAYDPAEWRR
                     LIEVNLIGTYEVTRQVLPHMRARPNGRIVNIASLAGKEGTPNSSAYSAAKAGVIAFTK
                     ALGKELADTDIRVNCIAPAAIETGLLSQMTREHLEIMIGKSPMKRLGTVAECAELVLW
                     LASEACTFNTGAVFDLSGGRATY"
     gene            complement(10241..11497)
                     /locus_tag="VF226_21895"
     CDS             complement(10241..11497)
                     /locus_tag="VF226_21895"
                     /inference="COORDINATES: protein motif:HMM:NF022773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HEX7076703.1"
                     /translation="MPFTRRTALKLFGIPPVAYMLGPGGLAAAAAEDEATLTPARRDE
                     LLSDARAAWEFFEQPGKQMTGLVPANIWPEDDSYGSYRILTMWDVGSILLATMSAHAL
                     GIIDDETRLERIAGIDRFLRKATYRYRGAKLPNFRSNIDTAKSVESGYDATDTARLFV
                     ALHVADRTKVPGIDSRALISSWDLARTIRNGQICGIKNGKMEPADFYVYRYYVSRAYT
                     LWNIDHKPVISGEPPNASEAARQAFVKELGSIGPISSEPSLAEEVELGPSPYSQGIVR
                     LLETAQLKRYKETSRLTCVSESPMDKEPWFTYQGYDLTREGEAAWTVYPWKTQKRWDT
                     PDFASKFRMVSSKAAFLWYATRPNAYTEKLWLHVREKARAPKYGFYPGVYERTGEPSR
                     NIDVNTNATILEAVAYALRGRKPLLS"
     gene            11864..12223
                     /locus_tag="VF226_21900"
     CDS             11864..12223
                     /locus_tag="VF226_21900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076704.1"
                     /translation="MATAIAVGGLYYVRDHNLRGASESVHAEEQSDYLRIVDRSAAYH
                     FGFVNAALAGCPFSPGTGLDRLAESVARNDQGIVPAELKAGFAAFQNLEPKRACRIAE
                     RLFGPKSPTRPGVLLPR"
     gene            12487..>13317
                     /locus_tag="VF226_21905"
     CDS             12487..>13317
                     /locus_tag="VF226_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076705.1"
                     /translation="MGIGEKNQVFFDPKGRRGTILKLVTWALGSTAAFISACLAFSVF
                     DTPILPEVKFALQANQLRPAAIFSGPLSDQIAVELGHRRTVPAGISESILRYGFFNHW
                     DESSFASLRENADKLDVLVPEWLHLAGADGDISHDDQRREDHVRLWLKKTARHLKVLP
                     LVNNFDPARGLWDGEGVKALLESPEARAKLTRNLLSYVQDGGFAGIVIDFKSLPDDAV
                     GAYVGFLDELHDAFSAVNLEVGAVLPAYEERFGAEDLSAVTDRIILLAYDDHWEGEAA
                     G"
BASE COUNT         2357 a         4181 c         4264 g         2515 t
ORIGIN      
        1 cgcggctcgg cgctgccgtt ctcgaaagag ctgcggggca cgttgatgct gacgagagcc
       61 gtcgcagacg gctcccggaa cagcaggatg tatttcatat aagggccaga tgccgtctgc
      121 tgctcgcccg tcacgtagac atactcgcct tgccggtcga gcgagccggt ctgaacctgg
      181 cccacaccct ttttcgaaag ggcctctgga ctgaggccac tggccatgtc ggggtaggct
      241 gacgcgggca cctcgagcag caggatcgat gcgccgttcg cgacgccaac gaacccggag
      301 aagtcgggcg aggccgcaaa tccgtccggc agttcgagaa cgacgcggct gccgggtgcc
      361 tgcgctgggg ccgcgatcaa gggggagggc gcaagcagca gtgccgcgag cagcagcacc
      421 gctatcgcct gcgctcgata tgggacctga tgggcggaca gcttcatgag ccggatttcc
      481 tctgctcgtg cggagggcca attccaagcg cgcgggtctg gttcccatcg atcgcctggg
      541 gaccggaaca ggcagccggc aaggggagcc tttgctttgc ttccccgccc gcataatcgg
      601 cgcgtgggag ccttcacttg atggccggaa agcgcccttc gatggggtag cccggatcat
      661 tgtagccggg cgtcgacgga tgccccgtca ccacgtggcg atcaacgatc gcctcgtcct
      721 cgggcgtcca ggtcacgtcc agcgcatcga ggtaaggctt ccactgctcc agtgtgcgtg
      781 gcccggcgat gacggacgag atggcttggt tgttgagcac ccaggcgacg gcaaaggcga
      841 tcagcgacag accgcgcgcc tccgcgtgtg ccttgagcgt ctccgcgatg gcgagcgact
      901 ctggccgcca ctccgtctcc atcatgcgct tgtcctggcg ggcggcgcgg ctgccgggct
      961 cgggcgtcgc gttgagccgg tacttgccac tcagcacgcc gcgggcgatc gggctgtacg
     1021 ggaccacgcc gagactgaac gcgcgagccg ccggcagcac ctccacctcc ggctggcggt
     1081 tcatgagatt gtaatagggc tgcagtgcca ccggttcggg ctcgttcagc gcgcggcaga
     1141 gatggcagac gtgcgcgatc tgccagccgc ggacgttcga caacgcccaa tgccggatct
     1201 tcccctggcg gatgaggtcg ccgaaggtcc ggatcacttc ttcccagggc gttttcggat
     1261 cgacgcggtg cgtatagtac acgtcgatga cgtcggtccc cagccgcttg aggctcgctt
     1321 ccaccgcctg catcacgtga atacgtgaca ggccgcgctc gttggggccc ttgccgtcca
     1381 ccggctgcgc caccttggtg gcgaggatga cgcgatggcg ccgtccctcc agcgcgcggc
     1441 cgaggatctc ttcggagcgg ccgtccgtgt agacattggc cgtgtcgatg aagttgacgc
     1501 cgtgctccag tgcatcatcg atgatccgcc gtgcctccgg ttcgttcgtc ggcccgccga
     1561 agttcatggt gccgaggcac aactcactga cgctgatgcc ggaccggccc agtacgcggt
     1621 agcgcatctc tgccatggcg ttcctttcgc ggggttgcgg tttccggccc agcttacacc
     1681 atcccaaccg atcccgagcc gcaggtgggc gagggctagc cgtagctcac aaatgccgga
     1741 tcacgaagcg gtagacgccg ccctgctctg agtgctccac catctcgttg cccgtcgact
     1801 tgcagaacgc ctgaaaatcg gcaacggcgc cagggtcggt ggcgagcacc tcgagcgtgc
     1861 cgcccttcgg cacctcggca atggccttct tcgtcttcag aatgggcagc gggcaattca
     1921 gacccttcgt atcaagtgtc ttgtcggcca ttgaaatctc caggagagca gcgcaggcag
     1981 attggttttc gcaaaccgta tatccagggg agcccggcgc gtcgagatgg cagcgggagc
     2041 aaatagagag tagcgaatag cgaatagcga atagcgaata ggagagcaca tgagccgtgc
     2101 gcccgagccc gtgctcctct acgcgctgtc acctggtacg ttcggctcgc cgatgaagct
     2161 caccggatgc tgcgggcgct cgtccacgat cagcttgaaa acgtccggcc gcgagtagtg
     2221 cccgacggcg tcgaaatcca gccggctgcg cggcacgtcc gcgagatcga gatcggcgta
     2281 gatgatggtc tcctcatcga acacgggccc ggcaaggacc ttgccgagtg gatggatgat
     2341 gcagcttccg ccgcgcatga cgacctgatc cgcatccatc tcggcagcga gtgcgtaatc
     2401 ggccggataa tcccggcggc ggttcatctg acaggccgag agcacgaagc agcgcccctc
     2461 cagcgcgatg tgctgcatcg tcggcaccca cgtcggccga tcgtccgccg tcggggcgca
     2521 gtagagcgtg atgcctttgg agtacatcgc cgtccgcagc agcggcatgt agttctccca
     2581 gcatatcact gccccgagcc ggccgtgcgg cgtgtcgaat acgggcaggg tggatccgtc
     2641 cccaaagccc cagataaggc gttcgcttgc ggtcggcatg agcttgcggt gcttgccgag
     2701 caagtctcca ttgggggaga aaaagagcac ggtgcagtag agcgtgcccc ggtcgcgctc
     2761 gatcaccccg atcaccagat gcaccccgtt ctcgcgtgcg gccgtgccca ggcggtccgt
     2821 ggcgggacct ggcacgtcga tcgcgctctc ccagtaccgg cggaagtcct ctcgcccctc
     2881 gggcttgcgc tggccgacac ggcagccgaa gtccagcccc ttcgggtagc cggagacgaa
     2941 ggcctccggg aagaccagca acttggctcc gcccgcggcc gcctcggcga tcagccgctc
     3001 ggccttggcg acagtggctt cccaatcgaa aacgacggag ctggcttgga cgacggctgc
     3061 tctcaactgg gacatggcgg atacctcgaa ctattcaaat taggtgcgga ggttggcggc
     3121 gtgcacggga tgctggcggg ggacatcgtg gaaatgttcg acgccttact ccttggcgtt
     3181 ggaagcgatg cgccgtgcct gacgataccg ccgtcgcgct cgttcagcca ggggcgatgc
     3241 aacctgccca ctgacccctg cgcaaccgac ggcctccgat cagcgatcct ttctcgtggc
     3301 ggatcgtgtc acagttccgc aacaggcggg ggatatctgg gggatatctg gagggctcct
     3361 cggcggatta ggcgcctttc gaaccctgga ccgtcgagaa aggtgaattg cgagtttccc
     3421 cgccgcactt gccctcgatc ggcacggatg gcaagcatcg gggcggaggc tcagtgagtc
     3481 gggcttgttg gccgcttatg aatgaccacg tttccgagcc acgccccggg ggaggggccg
     3541 gatcacgcat gcccgagcat ggcacgccaa aacgctcgtt cgccctgagc ttcggcctcg
     3601 accggctggg gctcgtcgcg ctgaaagccc cgcgggtctc ggcactcgtc atcttgatcc
     3661 tgagcgcatt ggccgtcgtg ggcgtcatgc ggctcaaggt cgatgactcg ctttccgagc
     3721 tcttccgcac ggacacgccg gaatttcacc agtacgagga gatcgatcaa cggtttccat
     3781 cgagtgaatt cgatgtgctc gccgtcgtcg agggtccgaa tctgctcgcc aggccgcagc
     3841 tcgaggcgtt cgcaaacgcc gtcatcgacc tgcaactagc ggatggcgtg gatgggctcg
     3901 tctcgatgct ctcggcgcgc ggcaagccgg atgccaccgg ctatgcgccg ccgattgttc
     3961 ccgatgagct gcccgacggc gcggcatacg acgagatgat caaggcgctg cgctccaacg
     4021 atatcgtggc cggcaagttc ctgtccgatg atggtcaact ggcgctcatc gtcatgtcac
     4081 tcaaccggca ggtcgtagag gagcggggcg ccaaggccgt catcggcaat attctcgaaa
     4141 cggcggagga ggcgctcaaa ggttccggcc tcaccgtgaa gctcaccggc gcgcccgtca
     4201 tgcagctcga gatccgcaat gcggtggagc gcgacaggct cgtttacaac gggctgggat
     4261 tcctggtcgg cgcaggtatt gccttcttgt tcttccgccg gttgtcgttg accctgatcg
     4321 ctgtcctcgg tccgtcgact gccatcctgt ggacgctcgg cgtggtgggc ggtctcgatt
     4381 tccgcctcaa cctgttcatc aacgtgctga cgccgctgat tctggtgtcc ggcttctccg
     4441 actcgatgca cctggtgttc tcgatccgcc gggacatcat cgccggcgtc gatcgcgtga
     4501 cggccgcgcg caacgccgtg ctcgacgtgg cgccggcctg cctgctgacc gcgatgaacg
     4561 catgcctggc gctcgtttcg ttctcgtggg cggagtccgc gctgatccgt acctttggca
     4621 aggcagcgct gttggcggtc ggcacgtcct acatcgccgt cgccgttgtg gtgccgacgc
     4681 tggcggcgct gctgatccgg cgcgaaaagg cgccggtcga aacacttgcc gagcggcgag
     4741 gcggtggcgt caacctcctc acgcgcctgt ccgactggat cgtccatcgt atcgcgtttc
     4801 gaccaattcc ctatttcctt ctgagcgtcg ccgtggtggt ggcctcaggg ttagcttacg
     4861 cgcagctgga gccgcactac cggctcgccg accaggtgcc ggacaaggag cacgcgcttg
     4921 ccgccacgga tcgcctggac cagaagctta cgggcgcgaa cccggtccac gtgatgatca
     4981 agctcaaggg cgggcattcg ctctacgagc cctcgacatt cgaggtcatc tcacgcgccc
     5041 ataccgtact ggagaaagtg gcggggctcg gtaacgtctg gtccgtggac agtttgcgcc
     5101 ggtggctcgc cgaggccggc gacgatcgca tcgagaccgt gaggtcctac atcgccgtcc
     5161 tgccggagca tctggtgcgg aggttcattg acaaggacga gaaggccgtc ctcgtaacgg
     5221 gccggctgcc ggacgtcgat gccagcgaaa tcctgccggt ggtcgagaag atcgaccagg
     5281 cgctggagcc ggtccgccag gagttccggg actacgagat taccgtgacc ggcttgcccg
     5341 ccattgccgc gcgcaactct gccaagctca tctgggagct gaacatcggc ctcgtcggcg
     5401 acatgttcgt catcttcatt ttccttggga ttgcgctgcg ctccgtcctg tccggcgtcg
     5461 ggagcatctt gccgtcactc tttccgatct tcgcaacggg cgcgctgctt tgggcaactg
     5521 gggagggcct gcagttcgca tccatcatcg ccattacggt ggcgttctca ctggcgatcg
     5581 attcgacgat ccatttcctc aatcgcttcc gcatcgagga aacccggatc ggttccggtc
     5641 ctggcaatgc cacgacggcg ctcgtcgccg cgatgcatca catcggaccc gtgatcgtgc
     5701 tcacgaccat cgtgctggcg atggggctcg gcgtgaccgt gctctcggat ctgccatcgc
     5761 tccggctgtt cgggcggctt gccgcggttt gcctgttcgc atcgctgatc ggtcaacttg
     5821 tcattttgcc ggcgacgatc gcgctctacc gccgttattt cccgctgaag gcgtgaagat
     5881 cgagctctga gtgacaggaa atcttcccca gtgccactgc tgtcctctcc attggctatc
     5941 gacgaggcat ggatcgatta caacggccac ctcaacatgg cctattacaa cgtgctcttc
     6001 gaccgcgcgc tggacgaggt gctgctgccg acgggactgg gtcccgacta tgtccgggag
     6061 acgggctgca cctacatggc cgtcgaggca cacatctgct atctgcgcga agtcttccgg
     6121 gcggatactg ttcgcgtcag cgtgcgggtg ctcgatctcg atgcgaagcg gatgcacctc
     6181 tattgtgaaa tgcgacacgc gatcgaggac tggctggccg cgacgtcgga atggatgttc
     6241 ctgcacgtcg acacgaagga gcggcgctcg gcgccctggc cgcccgacat tcgcgaacgg
     6301 ctcgatgcca tccgccgggc ggacgagcac ttggctccgc cggcacgggc aggccggcgc
     6361 atcggcattc cgcgcaagac ctaatctagc tagagccttt ccgattcaga cggaaacgcc
     6421 aacgataagc attccgttaa cctcttccat agggagagga cggcccgcag ggccgggtga
     6481 gggggtacaa catccgccac tggcggaacg ccctcacccc gaccctctcc ccgccgggga
     6541 gagggagatt gggcgcgagc ttgacgtgac gtgcttctgt ttagcgtccg gatggctcaa
     6601 ccgagacgag cgggtgaatg cgtagctttt ccaatcatcc ggttgccgct cgccgctcgc
     6661 cccaaaggtt gagcacgttg ccagcgatca cgacggcgcc ccccaacagg acgatcggat
     6721 cgaacgcctc cgcataggcc agcgcaccga cgactgcgat cagcggcagg cgcaaaaagt
     6781 ccatcggcgc gacgatgatc gcatctgcca gcgcgaaggc gcgcaccaga ccgtaatggg
     6841 ccgagagccc cgccagcgcg agcacgcaga tccagcccat ggtcgccatg tcaggcaggc
     6901 gaatgccgcc ggtcagcagg aaaagcgaca ggatcgcttg gatgaggaac atgtagaaga
     6961 ggatacgcag cgggctctcg ctgcgcgtca gcgtcttcgt ggcgagcatc gagagagcaa
     7021 agccgatcgc agcgatgagt gcgagaccga cgccggcatt cagtgccact gctccgggtc
     7081 gcgcaacaat cgtcatgccg atgaagccga ctgctgccgc tgaaagtcgc gtctgcgtca
     7141 ggcgctcgcc gaggagcagc ggcgccagca gggccaccca gatcggtgcg gtgaactcca
     7201 gcgcgaaaag ctgcgcgagg gggatcatcg tcagggcgct cagccagctg tattgcgcga
     7261 cgaagtgaac gagcgcgcgg accgaatgca gcgtgggccg ggtggtgcgc atctctttca
     7321 accccggccc catcagcacg atcgccagga tgacgatcag agagatcaga cagcggtaga
     7381 acatcatctc gagcgtcgtc gcaccgcgag ccgcctcgcg gccggcgacg gccacgctgc
     7441 tgaacgacgc gagagcaagc gccatccacg ccagcgcctg gagaacccgc ggctgcgttc
     7501 tggtcatcac cgcttccgac attctgcaaa ccttgtcccc ggggagcgct tgtccggcgg
     7561 tccccgactt cttcataagc tcgatatccg cttggcctga aaacgcgctt ctcggccggt
     7621 tcgtgtccag atagcggcgg ttgatgccga agtcacggac ttgaaggccg aatccctacg
     7681 ccgctgcaac ctgatgataa gcgaaccaga gcatcacccg cggcaaatcg ggttttctgg
     7741 attccgcgcg ggcgcacctg taaggtttca tcgagtgatc ggaaggactc ctcatgacac
     7801 gcatcacctc ggttgaagcc ctccgcgcca tctatccgcc gccgtccgag cgcgcactca
     7861 agaaacagct cgacaagctc gaccggcact gccggagctt catcgggctg tcaccgttcg
     7921 ttgtcatttc gtccgtcgga gccgacggac tggccgatgc ctcgccgcgc ggagatcggc
     7981 ccggcttcgt tcagatcctc gattatcaca cgatagccgt tccggactgg ccgggaaaca
     8041 atcgcctcga cacactcagc aacatcattg tgcggccgtc ggtcggactc ttgttcctgg
     8101 ttcccggcgt ggatgaaact ctccgggtga acggcgaggc agagctgcgg gacgacgagg
     8161 acctgcgctc tctcttcgag cgctcgggca agctaccgcg gctggccatc ctggtgcatg
     8221 tcaaggaagc ctatttgcat tgcgcgaagg ccctgatgcg ctcccgcctg tgggatccga
     8281 cctcccgcat cgaacgcagc ctgctgccga ccatgaacga gatgcttcag gatcagatcg
     8341 gcgccgcagg tcccatcgag ccgcaagagg acatgatcga gcgctaccgg aaggtgcttt
     8401 actgagaagg tgctttactg aaatgcgcca gcagttcgct tccgacaaca acgcggggat
     8461 gtgcccgcag gctctggctc gtttcctcga ggcgaacgct gccggtcatg cggtcggcta
     8521 cggcgacgat gcctggacgc aggaggcccg ggcgcgtatc gccgcgctgt tcgagaccga
     8581 gtgcgatgtg ttcttcgtat tcaacggcac ggcggccaat gcgctgacgc tggcgcaact
     8641 ttgccggccg tataatgccg tgatcggaca tggcttctct catatcgaaa ccgacgaggc
     8701 gggtgcgccc gagttcttca ccggcggcgc caagctgatg actgccgaca caccggacgc
     8761 gaagctgacg cctgaagccg tcgcggcgct ggcgacccgc tactcggggc cgcatcacgt
     8821 caagcccgcg gttctgtcgc tcacccaggc gacggagctc gggaccgtct acacgcccga
     8881 tgagcttcgt gcgctgacgg atgtcgcaaa acggcacggt ctcaaggtgc acatggacgg
     8941 cgcccggttt gccaatgcgg tggctcgcac cggctgcgcg ccggccgaca tctcctggcg
     9001 ggcgggcatc gacgtcctct gcttcggcgg cgtgaagaat ggcctcgcgg tcggcgattg
     9061 catcgtgttc ttcgatcgcg ccctgtcgca cgagttcgcg tggcgcatca agcaggcggg
     9121 gcagctcaat tcgaagatgc ggctcgtctc ggcggcgtgg ctgggactgc tggacggcga
     9181 cgtatggctc accaatgccc gtcacgcgaa cgccatggca gatcggcttg ctgcgggctt
     9241 ggcgcgcatt ccgggggtga ggttcatcgc gcccgtcgag gccaacgccg tcttcgccga
     9301 catccctccg gacgtgcagc gcgccctgcg agacaagggg tggcaatttt atactttcct
     9361 cggggagaca gggtgccgtc tgatgtgcgc ctgggacacg gaaccggcca ccgtcgacag
     9421 attcttgacg gacattgccg aggctgcggg ctcctagcca gccggctcag taggtcgcgc
     9481 gcccgccgga caaatcgaac accgcgcccg tgttgaaggt gcaggcctcg gaagcgagcc
     9541 acaggacgag ctccgcgcac tcggcgacgg tgcccaggcg tttcatcggg ctcttgccga
     9601 tcatgatctc cagatgctcg cgcgtcatct gtgacagcag ccccgtttcg atcgcagccg
     9661 gcgcaatgca attgacgcga atgtcggtgt cggccagctc cttgccaagg gccttggtga
     9721 aggcgatgac gccggccttc gcggcgctgt aggcggacga gttgggcgtg ccctccttgc
     9781 cggccagcga tgcgatgttg acgatgcgtc cgttgggccg cgcccgcatg tgtggcagca
     9841 cttggcgcgt cacctcgtag gtgccgatga ggttgacttc gatcaggcgg cgccactcgg
     9901 cgggatcgta ggcttccacc gagacggttg ggccgacgta gccggcattg ttgaccagga
     9961 tgtcgacgcc gccgtgcgtc gacagggttg cctcgagggc cgccgcaatt gcgtccggct
    10021 cggttatatc cacacggtgg ccgtccacac cgggaaggtc tggagccgca agatcccaga
    10081 ccgcaacccg ggctccagca tcccgaaacc ggcgggcaat ctcgcggcct atgccgttgg
    10141 cgccacccgt gacgacagcg acgcgtccag agaagtcata ggtgacgggc attggccaaa
    10201 atctccctgt attgcattcg ggagattctg gccctcgcga tcaggagagc aagggctttc
    10261 tgccgcgcaa cgcataagcg accgcttcga gaatggtcgc gttggtgttg acgtcgatgt
    10321 tccgcgacgg ttcacccgtt ctttcataga cgccagggta gaagccgtac ttcggcgccc
    10381 gtgccttttc gcgtacgtgc agccacagct tctcggtata ggcgttgggg cgcgttgcgt
    10441 accagaggaa cgccgccttg ctgctgacca tgcggaactt cgacgcgaaa tccggcgtgt
    10501 cccacctttt ctgggtcttc cacggatata cggtccaggc agcctcgcct tcccgggtca
    10561 gatcgtagcc ctgataggtg aaccagggct ccttatccat gggcgattcc gagacacagg
    10621 tgagacgcga ggtctccttg taccgcttga gctgtgccgt ttcgagaagc ctgacgattc
    10681 cctgggaata gggggagggg ccgagctcca cttcctcggc caggctcggc tccgacgaga
    10741 tcggaccgat gctgccgagc tctttcacga acgcctggcg cgccgcctcg ctggcattcg
    10801 gcggctcacc cgagatgacg ggcttatggt cgatgttcca tagcgtgtag gcgcgcgaaa
    10861 cgtaatagcg atagacgtag aagtccgccg gctccatttt cccgttcttg attccacaga
    10921 tctgaccgtt gcggatcgtc ctggcgaggt cccaggagct gatgagcgca cggctgtcga
    10981 tgccaggcac cttcgtgcga tcggcaacat gcagcgcaac gaagagacgc gccgtgtcgg
    11041 tcgcgtcata acccgactcg accgactttg ccgtatcgat gttcgaccgg aagttgggta
    11101 gcttcgcccc gcgatagcga taggtggcct ttcgcaggaa cctgtcgatg ccggcaatcc
    11161 gctctaagcg cgtctcatcg tcgatgatgc ccagagcgtg cgcagacatc gtcgccagca
    11221 ggattgatcc gacgtcccac atcgtcagga tgcggtagct gccgtaggaa tcgtcctccg
    11281 gccagatgtt ggcggggacg agtccggtca tttgcttccc gggctgttcg aagaattccc
    11341 aggccgcgcg agcatccgaa agcagttcat cgcgccgggc tggcgtcaat gttgcctcgt
    11401 cctcggcagc ggcggcagcg aggcctcctg gaccgagcat gtaagcgact ggcgggattc
    11461 cgaaaagctt caatgcagta cgtcgcgtga atggcatggg ccgcccccag gttcgaaaca
    11521 gtcgttgttt tcgcgataaa ctgatgcaag agacgggcca atccgattcg ggggcgttgt
    11581 gccagcttta cgaaaaataa gacaaatcac aattcaattt gaaacagagt gggtgggtgc
    11641 acctgagaac tgctctgtta tgatacaaaa agatgctgca tgccaaatat gcgtcggggt
    11701 taggaatgtt gccaatctcg cccgcctgcg gcatctcttc gcgagtcgaa tggaaggcgg
    11761 gcacgcagcg tatcgactgc ggctctggca agccttggag cggtcgcgcc ggggcggagg
    11821 gcggcttatg gcgagggtga ggtttccact cgttggagtc tccatggcga ctgccatcgc
    11881 agtgggtggt ctgtactacg tgcgcgatca caacttacgc ggagcgagcg aaagcgtgca
    11941 cgcagaggaa cagtcggatt acctgcggat cgtcgatcgc agcgcggcct accatttcgg
    12001 cttcgtgaat gccgcacttg cgggctgccc cttctcgccc ggcactgggc tcgatcggct
    12061 ggcggagagc gttgcgcgga acgaccaggg gattgtgccg gctgagctca aagcgggatt
    12121 tgcggcgttc cagaatctgg aaccaaagag ggcctgccgt atcgccgagc ggctatttgg
    12181 ccccaagtct cccacccggc ccggagttct cctgccacgt tgagctaaat caacaaacat
    12241 tcaattccat cgaatttgtc ggaaaagtta gcacaaattt accaacgtaa gcgattggtt
    12301 ccccacccca gggtgagcct ggcccgggaa ttgccatgac cgcctgcagc agccgagggc
    12361 tgcggtagct acagctcccg ggggggaact gttcaagcga tttcgccaag aattacgccg
    12421 ctggcagatt ctgcctgtca ggcacacggc gcttcgcacc cctcccaaga agattgagag
    12481 tggtgcatgg ggattgggga aaagaatcaa gtctttttcg atccaaaggg tcgccggggc
    12541 acgatattga agctggtgac ctgggcattg ggatcgactg ccgcgttcat ctcggcctgt
    12601 ttggccttca gtgtcttcga cacgcccatt cttccggaag tcaaatttgc gctgcaggca
    12661 aaccagcttc gcccggctgc cattttctcc ggaccgttgt cggaccagat cgcggtcgag
    12721 ctgggccacc ggcggacagt gccggccggc atctcggaaa gcatcctgcg ctatggcttt
    12781 ttcaaccact gggacgagag cagctttgcc tccttgcgcg agaacgcgga caagctcgat
    12841 gtgctcgttc cggagtggct ccatcttgcc ggcgcggatg gcgacatcag ccatgacgac
    12901 cagaggcgcg aagatcacgt acggctctgg ttgaaaaaga cggcgcgcca tctgaaggtg
    12961 ttgccgctcg tcaataactt cgaccccgcg agaggccttt gggacggcga gggcgtaaaa
    13021 gcccttttgg agtcgcccga ggcgagggcg aagctgacgc gcaatctcct gagctatgtt
    13081 caggacggtg ggtttgccgg gatcgtcatc gacttcaaat ctctccccga cgatgccgtc
    13141 ggcgcgtacg tcggcttcct cgatgagctg catgacgcct tttcagccgt aaacctcgaa
    13201 gtcggcgctg tgcttccggc ttatgaagag cgcttcggtg ccgaagatct ttcggccgtg
    13261 accgatcgga tcattctgct ggcttacgac gaccactggg aaggcgaggc cgcaggg
//
LOCUS       DASVDU010000329         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_964050, whole genome shotgun sequence.
ACCESSION   DASVDU010000329 DASVDU010000000
VERSION     DASVDU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964050"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /gene="hisG"
                     /locus_tag="VF226_21910"
     CDS             <1..271
                     /gene="hisG"
                     /locus_tag="VF226_21910"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401305.1"
                     /note="short form of enzyme; requires HisZ for function;
                     catalyzes the formation of N'-5'-phosphoribosyl-ATP from
                     phosphoribosyl pyrophosphate; crucial role in histidine
                     biosynthesis; forms heteromultimer of HisG and HisZ;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX7076706.1"
                     /translation="TRRFFQSKGIGDYRIVESLGATEGTPASGTADLIVDITTTGSTL
                     KANGLKVLDDGVILRSQANLVASRVADWSDTARAARHQIVARIRG"
     gene            complement(275..2284)
                     /gene="recQ"
                     /locus_tag="VF226_21915"
     CDS             complement(275..2284)
                     /gene="recQ"
                     /locus_tag="VF226_21915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEX7076707.1"
                     /translation="MLNAADLPRGTGPVASKHYDTIRGAPSPREVSYPDHSEAAPGRQ
                     QPEPATVTAMDGGGLSDERPGGMAAASAILEKVFGYKQFRSHQAAIIETLIAGHDAFV
                     LMPTGGGKSLCYQIPALVRAGTGLVISPLIALMQDQVDALKQVGVRAAFLNSTLRREE
                     QDEIESQLAAGKLDLLYIAPERLLQERTLNLVARSRIALFAIDEAHCVSQWGHDFRPE
                     YRQLRALAERFPHVPRVALTATADQRTRAEIVSELSLENARQFIASFDRPNIRYQIAQ
                     LDGRTAREHLRRFILTEHPTDAGIVYCLSRRSAEETAAWLTAQGRTALPYHAGLPADV
                     RRETQERFLAEDGLIVVATIAFGMGIDKPDVRFVAHLNLPKSIEAYYQETGRAGRDGE
                     PASAWMAYSMQDVVQLRDWIDKSDGGEVFQRVQRQKLDALVGLCELATCRRQALLGYF
                     GEELTEPCGNCDNCLAPPHMVDQTIAAQKALSAVYRTGEQFGVSYLTDVLLGRQDARI
                     ARNRHDKLSVYGIGDELNAAGWRRLFRQLVIGGQLAVDSEGHGSLLLTDKARPLLRGE
                     QKFLVRHAPKDVDSKVRRKRRQELAIPRANAPLYWELKAARARLASDAGVPPYVICHD
                     RTLLELAERRPASKDALAGITGLGQAKIERYGAALLEVISRFSRV"
     gene            2388..2669
                     /locus_tag="VF226_21920"
     CDS             2388..2669
                     /locus_tag="VF226_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076708.1"
                     /translation="MLMLRVSAILFGAAGLGTLWLAPDASAQANCDWYAKTALKQQQE
                     NEQRKCGFQGPEWSLDLSAHLNWCRGVAPDMWKKQAQLRNQQLEACAKK"
     gene            complement(2760..>3250)
                     /locus_tag="VF226_21925"
     CDS             complement(2760..>3250)
                     /locus_tag="VF226_21925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HEX7076709.1"
                     /translation="KVAMVLDGGPTEHGLESTVVDATGEKIFLLRPGAVTREAMESVL
                     GEPIARAGGTADSGDASGPRSPGQLASHYAPRARVRLNAQELRPGEVLLAFGEPLPGS
                     EGRCENLSPKGDLIEAAANLFAALRRLDATGAETIAVMPIPETGLGEAINDRLRRAAA
                     GR"
BASE COUNT          538 a         1034 c         1109 g          569 t
ORIGIN      
        1 gacacgccgg ttcttccagt cgaagggtat cggcgactac cgcatcgtcg agagcctcgg
       61 tgcgacggag ggcacgcctg cgtccggcac tgcggatctc atcgtggaca tcaccacgac
      121 cgggtcgacg ctgaaggcca atggtctcaa ggttctggac gacggcgtga tcctgcgctc
      181 gcaggcgaat ctggtcgcgt cccgggtcgc ggactggtcc gacaccgccc gcgccgcgcg
      241 gcatcagatc gttgcgcgca tcaggggctg agaatcagac gcgcgaaaag cgcgagatca
      301 cctcgagcaa tgcggcgccg tagcgctcga tcttcgcttg gccaaggccc gtaatgccgg
      361 cgagcgcgtc tttcgaggcc gggcggcgct cggcaagctc gagaagtgtg cggtcgtggc
      421 agatgacgta gggcggcacg ccagcgtcgg atgccagccg ggcacgggcg gccttcagct
      481 cccagtacag gggcgcgttg gcacgcggga tggcgagctc ctggcgccgc ttgcgacgca
      541 ccttgctgtc gacatccttt ggcgcgtggc ggacgaggaa tttttgctca cccctgagaa
      601 ggggccgcgc tttatcggtc agcagaaggc tgccgtgacc ttcggaatcg actgcgagct
      661 ggccaccgat gacgagctgg cggaagagcc gccgccagcc ggcggcgttc agctcgtcgc
      721 caatgccgta gaccgacagc ttgtcatgcc ggttgcgcgc gatgcgggca tcctgacgcc
      781 ccagcaagac gtcggtgaga tagctgacac cgaattgctc gccggtccgg tagacggccg
      841 agagggcctt ctgcgcggcg atggtctggt ccaccatatg cggtggcgcc aggcaattgt
      901 cgcagtttcc gcatggctcg gtcagctcct cgccgaagta gccgagcaag gcttgccggc
      961 ggcaggtcgc cagctcgcag agaccgacca gggcgtcgag cttctgccgc tgcaccctct
     1021 gaaagacctc tccgccgtct gacttgtcga tccagtcccg aagctggacg acatcctgca
     1081 tgctgtaggc catccatgcg ctggcgggct ctccgtcgcg gccggctcgg cccgtttcct
     1141 ggtagtaggc ctcaatcgat ttcggcaggt tgaggtgggc aacgaagcgc acgtccggct
     1201 tgtcgatgcc catgccgaaa gcgatggtcg cgacgacgat cagcccgtcc tccgccagga
     1261 agcgctcctg cgtctcgcgc cggacatccg ccggcagacc cgcatggtag ggcagcgcgg
     1321 tgcgcccttg cgcggtcagc cacgccgcgg tttcttcggc gctcctgcgg ctcaggcagt
     1381 agacgatgcc tgcatccgtc gggtgctcgg tgagaatgaa gcggcgcaga tgctcccggg
     1441 ccgtcctgcc atcgagctgg gcgatctgat agcggatgtt gggccggtcg aagctggcga
     1501 tgaactggcg cgcgttttcg aggcttagct cggaaacgat ctccgcgcgg gtccgctggt
     1561 cagcggttgc cgtcagagcg accctcggga catggggaaa gcgttcggca agcgctctga
     1621 gctggcgata ttcgggacgg aaatcgtggc cccattgcga aacgcagtgc gcctcgtcga
     1681 tggcgaacag cgcgatgcgg ctgcgcgcga cgaggttgag cgtccgctcc tgcagcagac
     1741 gctcgggcgc gatgtagagg aggtccagct tgcccgcggc gagctgagat tcgatctcgt
     1801 cctgctcctc gcgccggagg gtcgagttga gaaaggcggc ccgcaccccg acctgcttca
     1861 gggcatccac ctgatcctgc atcagcgcga tgagcggcga tatgacgaga cccgtacccg
     1921 cgcgaacgag tgctgggatc tggtagcaca gcgacttgcc gccacccgtc ggcatcagca
     1981 cgaaggcatc atggcccgcg atcagcgtct cgatgatcgc cgcctggtgt gagcggaact
     2041 gcttgtagcc gaagactttc tcgagaatcg ccgatgccgc ggccatgccg ccgggccgct
     2101 catcggacag acctccgccg tccatcgccg tcacggtcgc cggctcgggc tgttgcctgc
     2161 caggcgcggc ctcagaatga tccgggtagc tgacctcgcg cgggcttggc gctcctcgga
     2221 tcgtgtcgta gtgcttgctg gcaacgggtc ccgtccccct cggaaggtct gcagcattca
     2281 gcaccggttc atgccacccc ggctagtgtg caagcaagac atccggactg gccttgcaca
     2341 ggaggcggtg atccaagtcg caagttcagg gcgcgaggaa gacgaacatg ttgatgcttc
     2401 gggtgagtgc cattcttttt ggagcagcgg gtcttggaac gctgtggctt gcgccagatg
     2461 catcggccca ggcgaactgt gattggtatg cgaagacggc tctgaagcag cagcaggaga
     2521 acgagcagcg taaatgcggc ttccagggcc ctgagtggag cctcgatctc agcgcgcacc
     2581 tcaactggtg ccgcggcgtt gctcccgaca tgtggaaaaa gcaggcgcag ctgcgcaacc
     2641 agcagctgga ggcctgcgcg aagaagtgag ctgcgcggga gtggaggagg gcggcccagt
     2701 ccgccctgca gctcacgagg ggccgcatct ttcgagatgc ggcctttctc gtttggagcc
     2761 tatcgacccg cagcggcccg gcgcagacgg tcgttgatgg cttcgccgag gcccgtttca
     2821 ggaatcggca taacggcaat ggtctctgca ccggtggcat cgaggcggcg gagggcggcg
     2881 aacaggtttg ccgcggcttc gatcaggtcg cctttggggc tcaggttctc acaccggcct
     2941 tccgagcccg ggagcggctc gccgaacgca agcaggactt ctcccggccg tagctcctga
     3001 gcattgagcc gtacgcgcgc gcgtggagca tagtggctgg caagctggcc cggggagcgg
     3061 ggaccagagg catctcccga atccgctgtc ccgcccgctc gtgctatcgg ctcaccgagc
     3121 actgattcca ttgcctcgcg cgtcacggca cctggccgca gcaggaaaat cttctctccc
     3181 gttgcgtcga cgaccgtcga ctcgaggccg tgctccgtcg ggccgccgtc gagaaccatg
     3241 gcgaccttgt
//
LOCUS       DASVDU010000330         9732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_292560, whole genome shotgun sequence.
ACCESSION   DASVDU010000330 DASVDU010000000
VERSION     DASVDU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292560"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1595)
                     /locus_tag="VF226_21930"
     CDS             complement(237..1595)
                     /locus_tag="VF226_21930"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7076710.1"
                     /translation="MAAARKLNISDLIDNNKVSRFQIMLFVLCAMSLIMDGFDVQAMG
                     YVAPEVIRELGMSGGQLGSILAAANVGVLVGSLFFTMLADKIGRRPVLILGTFFFSGL
                     AILTAEADTANALLWLRFIAGVGMGSIIPNATALIGEYSPKAKRVTLIMTITVGFTAG
                     AALGGFVAAWLMPLFGWRSVFYFGGIIPLIVGILMIFWLPESLQFLVVRRKLANVARW
                     LKRIDPSIRVEPGTEYVVEEKSKDGVPVVHLLTDGRATVTLLYWLVNFTNLVNLYFLA
                     GLLPTILSQNGLSSSTSASIGGAMQLGGTIGTFALAWLIARKGFTAMLTLTFAVAAVS
                     IAIIGNQFVLTIVPLLTLVAFIAGATIIGGQPGVNALGATYYPTYMRSTGIGWGLGIG
                     RIGGILGPLIGGALLDLQWSTQQLFLVFAAPAACSTLIMLALHVAMRAPRVVVPLEEE
                     PA"
     gene            complement(2566..3423)
                     /locus_tag="VF226_21935"
     CDS             complement(2566..3423)
                     /locus_tag="VF226_21935"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX7076711.1"
                     /translation="MTDDSTTAGSENGAPRGIQSIEIGFRLVQCLVKAPHPLSLKTLA
                     ERAGVSASRARFYLVSFIRLGLVSQDREHGHYELGPYARHLGLAAIQRMDVVRVSAQP
                     LRRLCDTLKEAVFLAVWGNRGPTIVLTLDGPREIPMSVRVGYVMPVLYTATGRVFLSY
                     LPRQATDTLIKAELAAALRERGRDAANEMAESIESVVQNCRSAGFACTESLLNPGFAG
                     VAAPVFDHRGWLAAVVTVIGLGDQLDVRPNGPNVLMLLRAAEEISKSLGFRGQGANET
                     PAMSESTVL"
     gene            3691..4869
                     /locus_tag="VF226_21940"
     CDS             3691..4869
                     /locus_tag="VF226_21940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7076712.1"
                     /translation="MTAAGSVIVAARRTPIGAFGGRLKTLAAPVMGAAVIGQVIVDAG
                     IDSAEIDEVLMGCVLSAGVGQAPARIAAIQSGLPVGVPCTTINKVCGSGMKTIMLGDD
                     LINAGASLVLAGGMESMSNAPYLLTKARAGYRMGHQEVLDHIFFDGLQNSSDGNMMGH
                     FAETTAAHYGFTRQMQDAFARTSVQRALTAIQSGALEAEIVPLSIQDGTETCEIDQDE
                     LPFKCDIEKIPRLRPAFHKDGTITAASSSSIADGAAALLLMSPREADRRGLEPIARIV
                     GHASHAQEPQWFTTAPIEAIRKLLQRVGWRLDDVDLFEINEAFACVTMAVIKDLDLQP
                     EKVNVNGGACAFGHPIGATGSRIMVTMLYAMKQRRLRRGIASLCIGGGEATAIAVECL
                     "
     gene            4872..6839
                     /locus_tag="VF226_21945"
     CDS             4872..6839
                     /locus_tag="VF226_21945"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEX7076713.1"
                     /translation="MRSASTADVLWTPSLQRQAASQCARFIEFVNHAEGTSISDYAAL
                     HEFSIRQPEKFWAHVWQFCDIKAERTWSQVLTDGDRMLDARWFLDSRLNFAANLLRYR
                     DERTALVFISENGHRVQMSFAELYREVGRVAAGLANAGTEPGDRVVAYMPNRIETVVA
                     MLAATSLGAVFSSCSMDFGINGVLERFAQIEPKVLFCCDGYFYNGKRIDRLPRLRHIA
                     EHLPSLRQVVVVPYLDEQPPLLDVLPQAEHYSDFGQPGTHVPFESMPFDHPVYIMYSS
                     GTTGPPKCIVHGAGGTLLQHLKELVLHVDLTRSDSIFYFTTCGWMMWNWLVTSLAVGA
                     KVVLFDGSPMYPSVDVLWDIAETEEVSIFGTSARYLVTLEKSGAQPRRNHDLSRLRLI
                     LSTGSPLLPSNYDYVYRDIKADAQLASISGGTDILSCFALGTPLKPVYRGQLQCLGLG
                     MNVAVFDDNGRALPPGTPGELVCTAPFPSMPVKFWNDPDGKRYHETYFSRFENTWCHG
                     DWAELTPEGGLIIYGRSDATLNPGGVRIGTAEIYREVEKFPEVAESLVVGQEWQGDVR
                     VVLFLRLHPDSTLDKELEERIRTHIRNNTSPRHVPAKIIQVPDIPRTLNGKTVELAVR
                     DVIHDRPVKNVDALANPEALEHFRGLRQLAV"
     gene            6895..8163
                     /locus_tag="VF226_21950"
     CDS             6895..8163
                     /locus_tag="VF226_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7076714.1"
                     /translation="MSTKANTRGERHAAISNRYHVGSTRNGPVAPESFTRIPITLQHR
                     DDSAYTETYGFTGNQGMVTLEGQLLKPPVRTSNTLFVFQHPTSTLHLLPMPIALAEAG
                     FDVLCGASRYAKNDSSLIMEKVVLDLGSWIKLAREELHYEKVVLVGWSGGGSLSLFYQ
                     AEAESPTITHTPAGDPVDLTIAGLQPADGVIFIAAHLSRAETLTEWLDPSVVDEANPE
                     QRDAQFDIYAPNALHQPPFDAEYVRTFRAAQVARNRRITAWVQTLLEDMKRRRGPEVE
                     RPFVVHRTMCDPRWIDPQLDPNDRRPNWCYMGDPRTVNVSAVGLARFNTLRSWLSQWA
                     YDLSNARSSVTAPRIRKTPVLQIENSADDAVPATHNPTIYQALATPHKEYYTLKGATH
                     YYLGQPDHLAQCIAYITSWVERNGLLAGHC"
     gene            complement(8597..8835)
                     /locus_tag="VF226_21955"
                     /pseudo
     CDS             complement(8597..8835)
                     /locus_tag="VF226_21955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222646.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8815..8919
                     /locus_tag="VF226_21960"
                     /pseudo
     CDS             8815..8919
                     /locus_tag="VF226_21960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(9077..9631)
                     /locus_tag="VF226_21965"
     CDS             complement(9077..9631)
                     /locus_tag="VF226_21965"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7076715.1"
                     /translation="MAEHGQYVACSAGVGKSFVASAFAHAACRADFSVRCFRLPRLVE
                     ELARYAAMQKRSGLYRQLARADLIVIDDFGLAPLADETVRDLLEILDDRYDRRSTLIT
                     SQLPVNQWHAYLGDRTVADAILDRLVHNSYRLVLKEYSMRVDESDLLFDSPITASHWT
                     DMRRNDGPTSAATMDRHRRNPQAA"
BASE COUNT         2080 a         2924 c         2770 g         1958 t
ORIGIN      
        1 acgccgatgc agacgttcct cgatagcaaa gcgctggcga aggagaagac gctcgcggcg
       61 taacactagc tgacagtgac gaagccgacc gacacccgga cagaaaacac gaactgtcag
      121 atcaagtatt gacttctaca cgcggtttga gcaagagcgt caccatgcag gttgaactcg
      181 acggcacttg ggttcagcgc catgttcgaa cggtgtaggg gcggcgccgg ccccgcctag
      241 gcgggctcct cctccagcgg taccaccaca cgaggcgctc tcattgcaac atgcaacgcc
      301 agcatgatta gcgtcgaaca ggctgctggc gcagcgaaca cgagaaatag ttgctgcgtc
      361 gaccattgca ggtcgagaag cgctccacca attaacggac ccaggattcc gccgatacga
      421 ccaatgccga gcccccagcc gatgccggtc gagcgcatgt aggtagggta atatgtagct
      481 cccaacgcat taacgccggg ctggcctccg attatcgtag cgcccgcgat aaacgctaca
      541 agagtcaaca gcggaacaat ggtgagcaca aactggttgc cgatgattgc aatcgagacg
      601 gccgccaccg caaacgtgag cgtcagcatc gcagtgaagc cttttctggc aatcagccaa
      661 gccagggcga aggtgccgat cgttccaccg agctgcatag cgcctccgat tgatgcagag
      721 gtcgaggacg acaatccgtt ctggctcaaa attgtgggca gcagcccggc gaggaaatat
      781 agattcacca gattagtgaa gttcacgagc cagtacagca gcgtgacagt agcccggccg
      841 tcggtaagca aatgcacgac aggtacgccg tccttgctct tctcttccac gacatactca
      901 gtgcccggtt cgactcttat ggacgggtcg attcgcttta gccaccgcgc aacgttcgcg
      961 agcttgcgcc tcacaacgag aaactgcaac gattcgggca accagaagat cattagaatc
     1021 ccgacaatta acgggatgat accgccgaaa tagaacactg accgccaccc gaatagcggc
     1081 atcagccacg ccgcaacgaa gccgccgaga gcggcgcccg ccgtaaaccc gaccgtgata
     1141 gtcataatta gcgtgacgcg ctttgctttc gggctatatt cgccgatcaa agcagtcgca
     1201 ttcggaatga tcgaacccat gcccaccccg gcgatgaatc gcagccacaa cagcgcattc
     1261 gccgtgtccg cttccgcagt cagaatggca agtccggaga agaaaaacgt gccgagaatc
     1321 aacaccggac gacgacctat cttgtccgcg agcatcgtaa agaatagcga gccgaccaac
     1381 acaccgacat tcgccgcagc cagaatgctg ccgagttggc ctccggacat accgagctcg
     1441 cggatgacct ccggggcgac gtagcccatc gcctggacat cgaaaccatc catgatgagc
     1501 gacatcgcgc acagcacgaa cagcatgatc tggaatcggc tgactttgtt gttatcgatc
     1561 agatccgata tgttgagttt tctcgctgcc gccatactaa cccccccgga cgctaaagtg
     1621 accaatacgc tgagtaggat cggtgccgta agggaagcca acccgagccg ccctgttgct
     1681 tcgcccgcgt ttgacttact gcgttacaaa tagcaatacg taccctcagt ccgttctgcg
     1741 cccagcacgt ctcaatggac agcgtcgcgt gatgatgaaa ggcgcgattg cgaccctcgt
     1801 aatgactctg ggcgaactcg gacttcaacg cgtggtagtc gcgttcggtc atccactgcc
     1861 ccatggcgcg ggccatccac cgcttggagg acgttctttc aggcaacgtc gacaacccgt
     1921 agtgcacgga ctttgtcttc cgttaccggc cattcgatca gtggccagtc ctccttccgt
     1981 gtccgatcgc cgctcgccac gcgaacctgc acttgaggag gcgcatacgc agtgtcccgt
     2041 tcgtctcttc tcggcaagtg atcgtgcgcc agcccctcgc cagcaactgc cgagcatcaa
     2101 ccgccgatcg gctgaggctc ggtatcacgc taagcctggt gcgccgtccc tcatgtggtg
     2161 agtcgttcgc cggcatctgc gccggttgaa actcacccca ctcacgccgg tggtctctgg
     2221 ccagcgctgc ttcgatctgc gcgcgcaatc tgtccttgta gtgcgaaact cgacctgctc
     2281 gagaacggct gccgaacgtc gctcgcggtc ctgcaggcga tgcattgact gatgcgctgc
     2341 acgtacgttt tgcgggcact cgcggcatgc gttcaatgct cttgcgttcc cccagggcag
     2401 gcagcatcag caccttttca tgtaccaacg cgccggcgcc ggtacgacaa ccctatccgc
     2461 gtgctccggc accctagtgt tcgcgccgtc gtaccgccca aatcgtcgat cgctcatctc
     2521 tctacctctt ggactgcacg aagaagtgcg gataacgaat tgttctcata atacagtcga
     2581 ctcactcatt gccggagtct cattcgcccc ctggccacga aatccgagag acttggaaat
     2641 ttcctcagcc gcgcgcaaca gcatcaggac attggggcca ttcggacgta cgtcgagctg
     2701 gtcacccaaa ccgattaccg taaccacggc ggcaagccac ccacgatgat cgaaaaccgg
     2761 tgcggccaca cccgcaaatc caggattcag caggctttca gtacatgcaa aaccagcgct
     2821 tcgacagttc tgcaccacgc tctcgatgct ttcggccatc tcgttggcgg cgtcgcgtcc
     2881 cctttcccgc aaagcggcgg cgagctctgc tttgatgagc gtgtcggtag cctgtcgagg
     2941 caagtagctc aggaaaacgc ggcctgtggc cgtgtacagg acgggcatca catagcccac
     3001 acgtacggac atcggaatct cacgcggccc atcgagcgtc agcacgatcg tgggtccgcg
     3061 gtttccccat acggccaaga aaaccgcctc cttcagcgtg tcgcacagcc gccgcaacgg
     3121 ttgagcagac actcggacga catccatgcg ctgaatggcc gcgagaccga gatgccgagc
     3181 ataggggccg agctcgtaat gcccgtgctc gcggtcctgg gaaaccagac cgagcctgat
     3241 aaagctgact aaatagaaac gcgctctgct cgcggagacc ccggcccgtt ccgccagggt
     3301 cttgagagac agtggatgcg gcgcctttac aagacattga acgagacgaa agccgatctc
     3361 tatggattga atgccccggg gggcaccgtt ctccgagcct gctgttgtgg aatcgtctgt
     3421 cacgtatgtt acgtatcgct gttcataatt cagcatagtt gcacgctata caatatatct
     3481 ggtaaactac tcagtatcaa attacggccg ctgaatactg ttgggggctg acgccattgt
     3541 caaatgagac gaaccgtgcg gttccagctt cggtcgatcg atcggccgcc ggcccgcccg
     3601 cagaggacgg tgtccgcccg atcaatttga gcctgcaatg gaagtcagtc agggagtgat
     3661 cgactacgcc tatacgtctg agacgacgtc atgaccgccg ccggctccgt gatcgtcgca
     3721 gcaagaagaa ccccgatcgg cgcttttggc ggacgcctga aaacactcgc ggcacccgtc
     3781 atgggtgcag ccgtcatcgg tcaagtcatc gtggatgcag ggattgactc tgctgaaatc
     3841 gacgaagtgc tgatgggttg cgtgttaagc gctggtgtgg ggcaggcccc tgcacggata
     3901 gccgcaatac aatccggttt gcccgtcggc gtgccctgta ccacgataaa taaagtctgc
     3961 ggctccggca tgaagacgat catgctcggt gatgacctga tcaatgcagg tgcaagcctt
     4021 gtcttggctg gcgggatgga atccatgtcc aacgcacctt atctgctgac taaggctcgc
     4081 gccggatacc gcatgggcca ccaagaggtt ttggaccaca tattcttcga tggcctgcag
     4141 aactcgagcg atggcaacat gatgggccac ttcgccgaaa ctaccgcggc gcattatggc
     4201 tttacccgcc aaatgcaaga tgcattcgcc cgcacttctg tccaacgagc cttgacggct
     4261 atacagtcgg gcgcgcttga agccgaaatt gttccgctta gcatccagga cggaacggaa
     4321 acgtgcgaaa tcgaccagga cgagttgccg ttcaaatgcg acatagagaa aatccctcgt
     4381 ttacgccctg catttcacaa ggacggtaca atcaccgcgg ccagctcatc gagtatcgcc
     4441 gacggtgcgg cggcgctgtt gctgatgagt ccgcgcgaag ctgatcggcg cggtctcgag
     4501 cccatcgccc gtatcgtagg tcatgcaagc catgcgcaag agccgcagtg gtttaccacg
     4561 gccccgatag aagcgatacg caagcttctg cagcgcgtgg gttggcgcct tgacgatgtc
     4621 gatctgttcg aaatcaacga ggcgttcgcc tgtgtcacga tggcggtcat aaaggatctg
     4681 gatctgcagc cggagaaggt caacgtcaac ggcggcgcgt gcgccttcgg gcatcccatc
     4741 ggtgcgacag gctctcggat catggtaacc atgctatacg cgatgaaaca acgccggctc
     4801 aggcgaggta ttgcttcgct atgcattgga ggcggtgaag caaccgctat cgcagtcgag
     4861 tgtctctagt gatgcgctct gcttccacgg cggacgtcct ctggacgcct tctttacaac
     4921 ggcaggccgc aagtcagtgc gcccgcttta tcgagttcgt aaatcatgct gaaggcacgt
     4981 cgatcagcga ctacgccgca ctgcatgaat tcagcatcag gcagcccgag aagttctggg
     5041 cgcatgtctg gcaattctgc gatatcaagg cggaacggac ctggtcgcaa gttctcacgg
     5101 acggcgaccg catgctcgat gcacgctggt tccttgacag tcggctgaac ttcgccgcca
     5161 atctgttgcg ttacagagat gagcgcacgg cactagtgtt catcagcgag aacggccatc
     5221 gcgttcaaat gtctttcgcc gagctctacc gcgaagtggg gcgggtcgcg gcgggcctgg
     5281 cgaacgcggg caccgagcct ggtgatcgcg tcgtcgcgta catgccgaac cggatcgaga
     5341 cggtcgtcgc catgttggcg gcaacgagct tgggcgcggt tttttcttca tgctcgatgg
     5401 acttcggcat caatggtgtt ctggaacggt ttgcgcagat cgagcccaag gtcctgttct
     5461 gctgtgatgg ctatttctac aacggtaaac gcatagatcg cttgccacgc cttcgccata
     5521 tagccgagca cctgcccagc ctgcgccagg tcgttgtcgt cccatacctc gacgagcagc
     5581 ccccgctact cgatgttctg ccccaagccg aacactacag cgactttggg cagccgggca
     5641 cccatgtgcc cttcgaatcg atgcccttcg accatcccgt ctacatcatg tattcctcgg
     5701 gaaccaccgg tccgccgaag tgcattgtgc acggggccgg tggaacgctg ctacagcatc
     5761 tgaaagagtt ggtgctgcac gtcgacttga cacgtagcga cagcatcttt tacttcacga
     5821 cctgcggttg gatgatgtgg aactggttgg ttaccagcct ggccgtgggt gccaaggtgg
     5881 tgttgttcga cggttcaccc atgtatccgt cggtcgacgt cttgtgggac attgcagaaa
     5941 ccgaagaagt cagtatcttc ggcacgagtg caagatattt ggtcacgttg gagaagtctg
     6001 gggctcaacc gcgacgcaac catgacttgt cacgcctgcg cttaattctt tcgacgggct
     6061 cgcctctgct gccgtcaaat tacgattacg tgtacaggga catcaaagcc gacgcgcaat
     6121 tggcctcgat ttctggtggc accgacatcc tttcctgttt tgccctagga acgccgttga
     6181 agccggtata tcgcggtcaa ttgcaatgcc tgggactagg tatgaacgtg gcggtatttg
     6241 atgacaacgg acgcgcctta cctcccggca cgccaggtga gttggtatgc accgcaccct
     6301 ttccctcgat gcctgtgaaa ttttggaatg atcctgatgg caaacgctat catgaaacct
     6361 atttcagtcg tttcgagaac acttggtgtc acggcgactg ggccgagctc acgcccgaag
     6421 ggggtctcat catctatggt cgctccgacg ccaccctcaa ccccggcggt gtccgaatag
     6481 gtaccgccga aatttatcgc gaagtagaaa agtttcccga agtagccgaa agcttggtgg
     6541 tcggacaaga gtggcaaggt gacgttcgcg tagtcctgtt cctgcggctg catccggact
     6601 cgacactcga taaggagctg gaagagcgca tccgcactca catacgcaac aacacgagtc
     6661 cgaggcacgt accggccaag attattcaag taccggatat acctcgcacg cttaacggga
     6721 aaaccgtaga gctggccgtg cgcgacgtca ttcacgaccg cccggtaaag aacgtcgacg
     6781 cgctcgccaa tcccgaggca ctcgagcatt ttcgaggctt acgccagctc gccgtgtaac
     6841 tgcaaacgtc gctattcaaa ctgtatgttt tagtctctcg gaagtggaat tccaatgagc
     6901 acaaaggcga acacaagggg tgagcggcac gctgccattt caaatcggta tcacgtggga
     6961 agcacccgta atggaccagt ggctccggag agcttcacac gcattccgat cacgttgcag
     7021 caccgcgatg attcggctta taccgagact tacggattca ccggcaacca aggcatggtg
     7081 accttggagg gacagctgct caagcctcca gttcgcactt caaacacgct attcgtattt
     7141 caacatccga catctacgct tcaccttttg cccatgccca tcgcccttgc cgaggctggt
     7201 ttcgatgtgt tgtgcggtgc aagccgctat gccaaaaacg acagcagcct gatcatggag
     7261 aaggtcgtgc tcgatctagg cagctggatc aaattggctc gcgaggagct ccattacgag
     7321 aaggtagtgc tcgtcggttg gtcgggcggc ggctccttgt cgctatttta tcaagcggaa
     7381 gccgagtcgc ctacaatcac gcacacgccg gctggggatc ctgtggatct gacaatcgct
     7441 gggctgcagc ctgcggacgg ggtgatattc atcgcagctc atctttcgcg ggcagagacc
     7501 ttgactgaat ggctggaccc atccgtagtg gatgaggcca accccgagca gcgcgatgca
     7561 caattcgata tttacgcgcc gaacgcgcta catcagccgc ccttcgatgc ggaatacgtc
     7621 cgcactttcc gagcggcgca agttgcgcgc aaccggcgca ttacggcttg ggttcaaaca
     7681 ctgctggagg acatgaagag gcgacgcggg ccggaagttg agcgtccgtt tgtcgtccat
     7741 cgaacgatgt gcgacccgcg ctggatcgat ccgcagctgg accctaacga ccgtcggcca
     7801 aactggtgct atatgggtga tccgcgcaca gtgaacgtca gcgcagtcgg cctagcgcgc
     7861 ttcaataccc tacgctcgtg gctcagccaa tgggcctacg atctatccaa cgcccgtagc
     7921 agcgtcaccg ctccgcgaat ccgcaagacc cccgtactgc aaatcgagaa ttccgccgac
     7981 gatgccgtgc ccgcaactca caatcccacg atttaccaag cgctcgccac tccgcacaag
     8041 gagtattaca cgctaaaggg tgctacgcac tattacctgg gtcaaccaga ccatctggcc
     8101 cagtgtattg cgtatatcac cagttgggtg gagcgcaatg gactgctggc gggccattgc
     8161 taatgtggtc aagctgtgga tgccttcgtt cgcggactgt cctaacttca gcagcgccag
     8221 gctgacgaaa tatgacgctg gcgaattcaa tgaagaggcg gcggtagctg catcgctgca
     8281 tcgctggagg agtacgaagc gcgaatcgct gcgcgcgagc gcaaagtcgg tcagcttacg
     8341 atggagaacg atctcctcaa aaaaaccctg ccgcccgctc gctcgccgag cggcgcgagc
     8401 tcctcgatcg tgagcggccc gaagggctca gccgatcgcg ggcgtgtcgc ctgatgaaca
     8461 tggctcgtag taccagctac tatcgccctc gcggtcgcca gcgtccgggt gagtctgtgc
     8521 tgattgagcg gatcgaggcc atctgagagc cgttctgcag aacatgaagc tgcggctgaa
     8581 ggcgctggag gcgaaggtcc agcgccagct ccaccaccgc ctgctcgatc tccggcgcca
     8641 ccacttcaga ttcggctgcc gccgcgacat ctcctgcagc gcgagctcgc cgcctttctc
     8701 gtacagctcc ttgaagcggt agaagctgtc gcgcgagtaa cccatgatct tgcaggcctg
     8761 gctcacgttc cccagctgct tcgccagctc cagcaggccc agcttcggtc gaatgatcag
     8821 ccggcctggg gtcaagtgcg ggtatcgaac gagctgcgca agcgagggct gtcgatttca
     8881 ccggcgggtg tgcgctgcgt gtggcagctg cacgagctgc ccgaatgctc atccctcgtc
     8941 gggacagaat cctgatttcc attaccgctt cctttgtcag catccaaggg ccgccctcca
     9001 cattccggac ggcccgaatt ctcaacaagg gcagatcagt agttttgtcc gaaaagggtc
     9061 agtttccgat gtccgctcac gccgcttgcg gattccggcg atgtcggtcc atcgttgcgg
     9121 ctgatgtcgg tccatcattc cggcgcatgt cagtccagtg cgacgcggtg atcggactat
     9181 cgaagagaag atcgctttcg tcaacgcgca tcgagtactc tttaagcacg agtcgatagc
     9241 tgttgtgaac gaggcgatct aggatcgcgt cagcgacggt tcggtcgccg aggtaggcgt
     9301 gccactggtt gaccggcagt tggcttgtga tcagcgtgga gcgtcgatcg tagcggtcgt
     9361 cgaggatctc gagtagatcg cggacggttt cgtcggcgag cggagcgagg ccgaagtcat
     9421 cgatcacgat cagatcggcc ctggcgagct gacggtagag cccggagcgc ttctgcatcg
     9481 ccgcgtagcg ggcgagctct tcgaccagcc gcggcagacg gaagcatcgg acggagaagt
     9541 cggcgcgaca cgcagcgtgc gcgaacgccg atgccacgaa gcttttgccg acgccggccg
     9601 agcaggcaac atactgccca tgctctgcaa ttcgagataa cggtccatta ccgctttcac
     9661 cctcgccatc gcgagcgact cggcgttgtt aagacgcaaa cctatcgtgg cgagcgcgcg
     9721 tatgtcatcg ag
//
LOCUS       DASVDU010000331         3573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_563199, whole genome shotgun sequence.
ACCESSION   DASVDU010000331 DASVDU010000000
VERSION     DASVDU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563199"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1872
                     /locus_tag="VF226_21970"
     CDS             <1..1872
                     /locus_tag="VF226_21970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HEX7076716.1"
                     /translation="TDLQLAADFEPATYEAWLALVAKVLKGSDFEARLVSSTLDGIRI
                     APLYTRADALPGAECAKPGEAPYTRGSKAAGEAAGWDIRQLHLAIDPKEANAAILEDL
                     RGGATTIALQVPAGDLDAALDGVQLDACPVALMAGERTPEAAAELEALWDERGIADDA
                     RHGHFNADPLGTLAQSGQLTQPLDQNIERAVSLAIKAERWPGVTALLADGNPYNAAGA
                     SEAQELAAMLSTLVAYLRACEGAGLPPERSLPKIAVALGVDDNQFLSTAKLRAARRLI
                     WRVAEACGAGSATAGVHTTAVSAWRMMARRDPWTNIMRTTIACAAAAFGGAQSILLLP
                     FTFALGKTDSFARRVTRNIQIVLQEESSLGRVADPAGGSWYVEKLTDDLARKAWELFQ
                     NIESKGGIAAALTSGALQDEIAAVAERRNKAIATGRVEVTGVSAFPLLGDDGVKAEPW
                     PQAMPLRPDPAVKIKPLRQSRLAEPFEQLRDAADADAAVKGRRARVFVAALGTPADHG
                     TRSTWVRNFLASGGIEALIGEDFTNSGDAGAAFADSGARVACIASSDAVYAELAEATA
                     QALKSAGASHVFLAGQPGDQEAALKAAGVDAFWFAGQDRIAALRELHHLLEIAPSGA"
     gene            2076..2627
                     /locus_tag="VF226_21975"
     CDS             2076..2627
                     /locus_tag="VF226_21975"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX7076717.1"
                     /translation="MTAEEFISWDGDGHPGKLELVNGVVRAMSPASGTHALLQSTIAG
                     LLRDHLRARKSPCRVGTEAPIRPRMRSNENVRAPEVAVTCVPKSKDEKFFPDPVLIAE
                     VLSPSNQRETWESIWACATIPTLQEVMVVDSERIHVAVYTKDGTGAWPEHPKVVEIGG
                     AVRLTSIDAEIAISDIYADTNLV"
     gene            2633..>3573
                     /locus_tag="VF226_21980"
     CDS             2633..>3573
                     /locus_tag="VF226_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7076718.1"
                     /translation="MTRIPDFTTVAFDAELSPDPGTGSSEATAQVWQTPEGIAVKPAY
                     TGEDIAGLDFIDGLPGIPPYLRGPYSTMYVTQPWTIRQYAGFSTAEESNAFYRRNLAA
                     GQKGLSVAFDLATHRGYDSDHPRVKGDVGMAGVAIDSIYDMRTLFEGIPLSEISVSMT
                     MNGAVLPVLALFIVAGEEQGVPPEKLSGTIQNDILKEFMVRNTYIYPPEPSMRIISDI
                     FAYTSRHMPKFNSISISGYHMQEAGATADLELAYTLADGLEYARCGVNAGLDIDSFAP
                     RLSFFWAIGMNFFMEIAKMRAARLLWANLIQTNFNPK"
BASE COUNT          640 a         1207 c         1107 g          619 t
ORIGIN      
        1 actgatctgc agctcgcagc cgatttcgag ccggcgacgt atgaggcctg gctggccctc
       61 gtcgccaaag tcttgaaggg cagcgacttc gaggcccggc tcgtctcgtc cacgctcgac
      121 gggatcagga tcgctccgct ctatacgcgc gctgatgcct tgccgggcgc ggagtgtgcc
      181 aagcccggcg aagctcctta tacgcggggc agcaaggctg cgggagaggc tgccggctgg
      241 gacatccgtc agctccatct cgctattgat cccaaggagg cgaacgcagc cattctcgaa
      301 gatctgcgcg gtggcgccac cacgatcgcg ttgcaggtcc cagcgggtga tcttgacgcc
      361 gccctcgatg gcgtccagct cgatgcctgc ccagtcgccc tcatggcagg cgagcgcacg
      421 ccagaggccg cggcggagct ggaggccctc tgggacgagc gcggcattgc ggacgacgca
      481 cggcacggcc atttcaacgc tgatccactg ggcacgctcg cccagtcagg tcaactcact
      541 caaccgctcg accagaacat cgaacgggcg gtctcccttg ccatcaaggc cgagcgctgg
      601 cccggcgtca ccgccctgct cgcggacggc aatccgtaca acgccgccgg cgcgagcgag
      661 gcccaagagc tcgccgcgat gctttcgacg ctggtggcct atctgcgcgc ctgcgaaggt
      721 gccggcctgc cgcctgagcg atcgctgccg aaaatcgccg tggcgctcgg cgtcgatgac
      781 aaccagttcc tctcgacggc gaagctgcgc gccgcgcgcc ggctcatctg gcgcgtcgcg
      841 gaggcctgtg gtgccggatc cgccacggcc ggcgtacaca ccactgccgt gagcgcctgg
      901 cgcatgatgg ccagacgcga cccgtggacg aacatcatgc gcaccacgat tgcctgtgcg
      961 gcggcggcat tcggaggtgc gcaatccatt ttgctcctgc ccttcacctt tgcgctcggc
     1021 aaaaccgatt cgttcgcgcg ccgcgtgacg cgcaacatcc agatcgtgtt gcaggaggaa
     1081 tcgagcctcg gtcgtgtggc cgatcccgcc ggtggcagct ggtacgtcga gaagctgacg
     1141 gatgatttgg cccgcaaggc gtgggaactc ttccagaaca tcgaatccaa aggcggcatt
     1201 gccgcagcac tcacctccgg cgccctgcag gatgagatcg ccgccgtcgc cgaacgccgc
     1261 aacaaggcca tcgccaccgg ccgcgtcgag gtgaccggcg tttcggcatt cccgttgctg
     1321 ggcgatgatg gtgtgaaggc ggaaccctgg ccgcaagcca tgccactcag gcccgatccg
     1381 gccgtgaaga tcaaaccgct tcgacagtct cggctcgctg agccctttga gcagctgcgc
     1441 gacgctgcag atgcggatgc agcggtgaaa ggtcggcgcg cccgcgtgtt tgtcgccgcg
     1501 ctcggcaccc cggcggatca cgggacgcga tcgacctggg tgcggaattt cctggcctcg
     1561 ggcggcatcg aagcgctcat cggcgaagac ttcaccaact ctggcgatgc aggcgcggcc
     1621 tttgccgaca gtggcgcgcg cgtggcctgc atcgcctcct cggacgcagt ttacgcggaa
     1681 ctggccgaag cgactgccca agcgctcaag tccgctggcg catcccacgt cttccttgcc
     1741 ggccaacccg gagatcagga agccgcgctc aaagccgctg gcgtggacgc cttctggttc
     1801 gccggccagg accggattgc ggcgcttcga gagttgcacc acctgctcga gattgcgcca
     1861 tccggcgcat gacgcgatat gtgatagtgt agtgtctgaa ggtagcccca cactctgggt
     1921 catcccggtg cttgtcaccg ggatccacct tgccgcttac gcctgtgtaa gcggttggct
     1981 ggatcccggg cacgaggccc gggatgacgg cgggagctga ggcactctag aacgaagacg
     2041 ttttgatgcc ggcccaggtt agtcagcagc atcgcatgac ggccgaggag tttatctcct
     2101 gggacggcga cggccatccc ggcaagctgg aactcgtcaa cggcgtcgtg cgcgccatgt
     2161 cgccagcgag cggcacgcac gcgctgctcc aatcgacaat cgcggggttg cttcgagatc
     2221 acttgcgagc acggaaaagc ccttgtcggg tcgggacaga ggcacccatc cggcctcgta
     2281 tgcgctcaaa tgagaatgtg cgggcaccgg aggtagcagt gacatgcgtg cccaaatcca
     2341 aagacgagaa gttctttccc gaccctgtcc tgatcgctga ggtgctctcg cccagcaacc
     2401 agcgcgaaac ctgggaaagc atctgggcct gcgccacgat ccctaccctg caggaagtca
     2461 tggtcgtgga ctcggagcgc attcacgttg ccgtctatac caaggatgga accggcgcct
     2521 ggcccgagca tccgaaggtc gtcgaaatag gcggcgcggt tcgcctcaca tccatcgacg
     2581 ccgagatcgc gatttctgac atttacgccg acaccaacct ggtttgacga gcatgacccg
     2641 catcccagat ttcaccactg tcgctttcga cgccgagcta tcgccagacc ccggcaccgg
     2701 tagctcggag gcaacagcac aggtctggca aacgcccgag gggattgccg tcaaaccagc
     2761 ctacacgggc gaggacatcg ccggcctcga cttcatcgac ggcctgccgg gcattccgcc
     2821 ttatctgcgc ggcccctact ccaccatgta cgtcacccag ccgtggacca ttcggcagta
     2881 cgccggcttc tccacggccg aggaatccaa cgccttctat cgccgcaatc tcgcggctgg
     2941 ccagaagggc ctgtctgtcg ccttcgatct tgccacccac cgcggctacg actcggatca
     3001 cccgcgcgtc aaaggcgacg tcggcatggc gggcgtcgcg atcgactcca tctacgacat
     3061 gcgcacgctg ttcgagggca tcccgctctc cgagatcagc gtgtcgatga ccatgaacgg
     3121 agcggttctg ccggtgctcg cgctcttcat cgttgcgggt gaggagcaag gggttccgcc
     3181 ggaaaagctc tccggcacca tccagaacga catcctcaag gagttcatgg tccgcaacac
     3241 ctacatctat ccgcccgagc cctcgatgcg gatcatctcc gacattttcg cctacaccag
     3301 ccggcatatg ccgaagttca actcgatctc catctccggc tatcacatgc aggaggcggg
     3361 ggcgacggcg gatctggagc tcgcctacac gctggctgac ggcctcgagt acgcacgctg
     3421 cggggtcaac gccgggctcg acatcgacag cttcgcgccg cggctgtcgt tcttctgggc
     3481 gatcggcatg aacttcttca tggagatcgc caagatgcgc gccgcccgcc tgctgtgggc
     3541 gaacctcatc cagaccaact tcaacccgaa gga
//
LOCUS       DASVDU010000332        40620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1536487, whole genome shotgun sequence.
ACCESSION   DASVDU010000332 DASVDU010000000
VERSION     DASVDU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 40620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40620
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536487"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VF226_21985"
     CDS             complement(<1..410)
                     /locus_tag="VF226_21985"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7076719.1"
                     /translation="MSNGPQQKIGFIGVGMMGHGICLNLIKAGFPLTVIAHRNRARID
                     DLLGRGAAEAKSLRELAADADVVMICVNSADSVAELVREMLPAMSAGKMIIDVTTSKP
                     ETSEMLAAEAKAKGVTFIDAPVVGGPPQAAEGKLG"
     gene            complement(438..512)
                     /locus_tag="VF226_21990"
     tRNA            complement(438..512)
                     /locus_tag="VF226_21990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Val,seq:tac)
     gene            complement(577..831)
                     /locus_tag="VF226_21995"
     CDS             complement(577..831)
                     /locus_tag="VF226_21995"
                     /inference="COORDINATES: protein motif:HMM:NF040427.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HEX7076720.1"
                     /translation="MPWSMCRHVERQAQLMGEMMERLGVDPLAAARHQSGASFAQART
                     NCLVCRSTSECRRWLDGVPGAEHPSEYCPNMAFFEACLRR"
     gene            1050..2342
                     /locus_tag="VF226_22000"
     CDS             1050..2342
                     /locus_tag="VF226_22000"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEX7076721.1"
                     /translation="MSDQPGAPRFDTLAIHAGAAPDPSTGARVTPIYQTTSYVFNDAD
                     HAAALFGLEVFGNIYTRIMNPTQAVLESRVAALEGGTAALAVASGHAAQFLAFHTLLE
                     PGDEFVAGRQLYGGSVNQFNHSFKKFNWNVIWADSTNPESFARAITPNTKAIFVESIA
                     NPGGIVVDLPAIAAIAKKAGVPFIVDNTFATPYLCRPKEHGADIVVHSLTKFMGGHGN
                     SIGGIIVDCGTFDWLGSKHRYKTLVDPNPSYNGMKLAETFGNFAFAIAARVMGLRDLG
                     PAIAPMNAFLILTGIETLPLRMARHCENAQAVAEYLVKHPKVAWVNYAGLPDNPSYEV
                     HKRVCPKGVGSVFTFGLKGGYDAGVKLVASLKLFSHLANVGDTRSLVIHPASTTHRQL
                     TDEQRIAAGAGPDVVRLSVGLEDKADIIADLDQALAQV"
     gene            complement(2790..4607)
                     /gene="phaC"
                     /locus_tag="VF226_22005"
     CDS             complement(2790..4607)
                     /gene="phaC"
                     /locus_tag="VF226_22005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX7076722.1"
                     /translation="MQKPNPDARSDATAYKLGNPEELALNLLKLFEQGGKVMAQLAER
                     ADTRLGPYSGVNEMAEAAKAMGEIARYWFGDPARLAEAQGELVRGYLDLWHSAIRRAL
                     GEEVEPVAKPAAGDNRFKDTEWSASPYFDFVKQMYLLTAQWAENVLQKTDGVDERTRM
                     KAEFYLEQLLSALSPSNFPATNPEVLRETLATSGRNLIEGLQNLAEDMESSGDLLKIR
                     QTDLEAFEVGRNLAVTPGKVVFQNDLFQLIQYTPTTETVYERPLLIIPPWINKFYILD
                     LTQPKSFIKFAVDQGFTVFVVSWVNPDERLKDKTFEDYMIEGALAATHAVRRETGQDK
                     INVLGYCVGGTLLATTLAYLAARGEELYRSATFLAAQVDFNHAGDLLVFIDDAQLTAL
                     EEMMAERGYLDGSRMASVFNMLRPRDLIWPYIVNNYLLGKKPFPFDLLYWNQDSTRMP
                     AANHRFYLREFYHENKLAKGQMKIAGTRLDLGKVKLPIYELATREDHIAPAKSVFVGS
                     KLFGGPVEFVLAGSGHIAGVINPPDPKRVKYQFWTNKKPAETLEEWIANAKETPGSWW
                     PHWAKWLAKHSGKRIPARKPGAVLGVIEDAPGSYVKVKG"
     gene            4774..5076
                     /locus_tag="VF226_22010"
     CDS             4774..5076
                     /locus_tag="VF226_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076723.1"
                     /translation="MRDCDFRLARRAPKLHERRLQKAVPAAAGIVLLAIGLASSLASC
                     SPGNSTRLPELSSIPRKLLSAEEQKKVVDELQRRRDTHREEAIKEIKSARAADPSG"
     gene            5194..6420
                     /locus_tag="VF226_22015"
     CDS             5194..6420
                     /locus_tag="VF226_22015"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX7076724.1"
                     /translation="MTDEFHRIKRLPPYVFAEVNRLKADARAAGADIIDLGMGNPDMP
                     TPKHIVDKLIETVGKPRTNRYSASKGIPGLRRAQAAYYERRFGVKLNPETQVVATLGS
                     KEGFANMAQAITAPGDVILVPNPTYPIHEFGFIISGASIRHIPASNGPEFLRGVERAA
                     KYSIPKPLAMVLSYPSNPTAMTADLDFYAEAVQLAKRLGLIILSDVAYAEIYFDDNPP
                     PSVLQVPGAMDVAVEFTSLSKTYSMPGWRIGFAVGNERLIAALARVKSYLDYGAFTPI
                     QVAAAAALNGPQDCVEEIRQIYKSRRDTLVESFSRAGWNIPAPPATMFAWAPIPEPYR
                     DIGSVEFAKLLIEKADIAVSPGLGFGEYGEGYVRIALVENEHRIRQAARNLRRFLSQP
                     PELMHNVIPMQQKASR"
     gene            6429..7754
                     /locus_tag="VF226_22020"
     CDS             6429..7754
                     /locus_tag="VF226_22020"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX7076725.1"
                     /translation="MSPPLKIGVAGLGTVGTGLLQVLEVHGGRLAATLGREIRVVAVS
                     ARSRDKARRARFEHFRWVADAVELATDPEIDVFVELIGGDSGVAAEAVEAALRAGKHV
                     VTANKALLAQHGIRLARLAEEKGVALNFEAAVAGGIPVIKTIREALLGNEISRVYGIL
                     NGTCNYILSTMEKEGRPFAEVLKEAQERGYAEADPTFDIGGFDTAHKLSLLTALAFGT
                     ELAFEDIHVEGIEHITPADMEAADDLGYRIKLLGVALRTPSGIEQRVHPAMVPKHSAI
                     AEVSGVTNCVAIDGDFAGDLLLVGAGAGAKPTASAVASDIGDIARGLVLPPFLTPAKQ
                     LKPYARAMLDRHQGAYYVRLTVHDRPGTMAAIAARMAEQDVSIESIVQRRPRSALPGT
                     GARPAPGTLTPVVLITHETTEEAMRKALAAIERDGKVSEMPQMIRIERL"
     gene            7799..8785
                     /gene="glpX"
                     /locus_tag="VF226_22025"
     CDS             7799..8785
                     /gene="glpX"
                     /locus_tag="VF226_22025"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX7076726.1"
                     /translation="MLVDLAEGLDRVFVLEVGRITEAAAIAAARLRGRGNEKAADKAA
                     VDAMRRELNRVHIRGRVVIGEGEMDEAPMLYIGEEVGIGDGPEVDIAVDPLEGTTICA
                     KSMPNALAVLAIAERGGLLHAPDMYMNKIAIGPGYPEGVIDLDAPPIDNLNALAKAKG
                     VPIGEITACILDRPRHAELIKAVREAGAAVQLIPDGDIAGVIWTTDPEETGIDIYLGS
                     GGAPEGVLAAAALRCIGGQMQGRLMPLKDEERERAAKMGADVDRKYSLEDMASGDVIF
                     SATGVTDGSLLDGVHFRGDFAETETVIMRAKTGTVRRIKTTFRNIGTKLAGL"
     gene            complement(8802..9608)
                     /locus_tag="VF226_22030"
     CDS             complement(8802..9608)
                     /locus_tag="VF226_22030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7076727.1"
                     /translation="MSSTIAFTFGIPLLPRLGARNWALIECLFKLTLDSILAQSDQDF
                     RIVVAGHDRPANFPDDPRIEFLQADWPAEPQRSDNLDRGRKVHAINALVMARGGGLLM
                     FVDADDWIDKHLVESARTLIGPDHIGGVTTAGFATDFRTLRTAKLPDPRIFAGGFHQL
                     CGTSTVAQLRPGHRDPLRRDPHRVLHEHYRWIELARDFGVPLVQLPSRNTYLINTSEN
                     HSETQGPFADWRREFTRAVNAFGAPLTSEMAASFGLELDRIRAASEAFRD"
     gene            9762..11240
                     /locus_tag="VF226_22035"
     CDS             9762..11240
                     /locus_tag="VF226_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7076728.1"
                     /translation="MRPLPGKPDCCWTVVAPETSYPPLQGKHAVDIAVIGGGIVGLTA
                     ALQIAKAGRSVAVVEALRVGRQVTGRSTAKITTQHRLIYDHLIRTVGREKAQLYADAN
                     RKGAEFIESCINEFAIPCDFERRSAITWIEADKEGLADIEAEAEAALSLGLPADVLDR
                     APLPFATGGGLRFTDQAQFNPALYCIGLAAAVVRSGGQVFERSRVIAVEREDLWRIRT
                     EAGEIRAESVFLATNLPVAGEVDFSHYVQARCHTAAAFRIAAERAPDGMFLSVGHPSH
                     SIRVGRDDDGLLLIVLGPHFITGQEGNVARRFLELEDWTRERFKVGDMVWRWTNEDYD
                     TADRIPYVGAPSAKEPGLYIATGFNAWGITNGTAAGLLVADQVLGRENPSAALYDPTR
                     PLPKSFHKGGKTQSKAADVSSIRLGEGGVVQIGDETFAVHKTMSGIVNALSASCTHQG
                     CTVTWNNADKTWDCPCHGSIFSADGSVLHGPAVEPLPSRKLP"
     gene            complement(11274..11660)
                     /locus_tag="VF226_22040"
     CDS             complement(11274..11660)
                     /locus_tag="VF226_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7076729.1"
                     /translation="MMEPSESYVPGQCQLVNDVISLVGDKWSVLIIVLLGAGKKRFSD
                     IKRAVDGISQKMLTVTLRGLERDGYVTRTVYPTIPPKVEYELTDLGRDLLVPLRALGQ
                     WAIANHARVSSARAAYDARQAREAAE"
     gene            11749..12330
                     /locus_tag="VF226_22045"
     CDS             11749..12330
                     /locus_tag="VF226_22045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX7076730.1"
                     /translation="MKTVKTVAVLVGSLRRESINRKFAESVGKLAAGRLEFRFVEIGD
                     LPLYNDDLWASPPESVLRMKREVEAADAVLFVTPEYNRYFSPAIKNAIDWGTRPWGEN
                     SWAGKPSAVIGTTNGATGTSAAQNSLKALLTVVETILMGQPEVYFTYKPELFDKDNNV
                     VDDSTRDFLNTWIDRFAAWIEHTGRAHIPAKAA"
     gene            complement(12409..13467)
                     /locus_tag="VF226_22050"
     CDS             complement(12409..13467)
                     /locus_tag="VF226_22050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEX7076731.1"
                     /translation="MRIRAKPHALGGWARAAALFACLLTGTAVAGDAALAAPAIRISA
                     ANPVPACATPERLMAFLRARNKDLDPRFKDIAVWYKRYGEALGVRWDYAFFQMLIETN
                     YLTFRRGNGKRGDVDPSQNNFAGIGATGGGVPGNSFPDVATGVRAQLEHLVVYSGERV
                     PNPVAERTRAKQDDILSWTAPLSRKRPVTFQDLAGKWAADRAYGRSIELTAERFRKTY
                     CNGDQIATAGKETHAAARAPAWQTTTARAGAAALAAPRPTRAIRPPAPEPRAAEPSAC
                     RVLMASYGGTKTMLIRHVAEDAVEYTALRVLDGFEETMTESYINAHAPGGTLIGEFSS
                     SEAAFARAFQLCPAADRG"
     gene            complement(13467..14663)
                     /locus_tag="VF226_22055"
     CDS             complement(13467..14663)
                     /locus_tag="VF226_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076732.1"
                     /translation="MKCRAAASLLLLTWTALPAMAQSLPAIKTGPGNQVAACATPGRL
                     MEFVRHRNASLDPRFDGVATEYMRHGEELGVRWDYAFFQMLLETGNLSFTRGNGQPGN
                     VRPSQNNFAGLGAAGRGQRGESFPDISTGVKAHMQHLLLYAGDYIADPVADRTRKVQE
                     WGILDSFQRKLNRPVTFTDLAREWAPGDRRYAKKMGAIAQAFFDDVCHRPDPRPELVA
                     EARRGRGGAVSTASTSTVNTRPKVSGTELARRAVENAREEGATRSSLGAASIAAKTAG
                     TSSMANQNTAISEAWQSGSSKVDGTAKLQTASAGAAAASAAKESAAPKCRVWTASYGG
                     QKSVIIKAVSDGLVNYTVLDVNEGREKRETEAYIAAYAKGGKTIGEFSNQLSALDRAF
                     ELCPEG"
     gene            complement(14864..15289)
                     /gene="folB"
                     /locus_tag="VF226_22060"
     CDS             complement(14864..15289)
                     /gene="folB"
                     /locus_tag="VF226_22060"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX7076733.1"
                     /translation="MSVDPWSDEALAEAERLPAVAGSRRVFVRGLELIGSVGVHELEH
                     RYEQRVVVSLDLEIRDGYDGASDHISEVYDYDLAIQAVKETLASGHFNLIETLAERIA
                     EACLSDPRVRSIRVRVEKPDVLPSCAAVGIEIERRKADY"
     gene            15598..16353
                     /locus_tag="VF226_22065"
     CDS             15598..16353
                     /locus_tag="VF226_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076734.1"
                     /translation="MVLTIAILVTLTVAIAAFVLAPRTARRRVLFDAAPDRPAPFGLK
                     MAWLAIRTQDTARVLDVLGMVDPRPCNWNSGIGTVYDDRLGEHHLFVSPPVDGWTFVV
                     GLSLPYPVGQGFVDKCTPMLLRLGREFGEVQYFFSYPLIDFFAWARIIGGKLVRAFAI
                     SDEGTIWNKGKPTKEERSLGLKLFELRGVRERTGDAGGALMLYPTEEHVMRLASRWSL
                     DPTRIDSAPASASTGYICLAPTSWRPERMRKSA"
     gene            complement(16389..16781)
                     /locus_tag="VF226_22070"
     CDS             complement(16389..16781)
                     /locus_tag="VF226_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076735.1"
                     /translation="MTTPPIFRAGERWTYRAPAGFEHSRIIVGAVVTFTDREPVICCA
                     VVGAPARRSDGSIETATIPFLPLSASALAASVVAPDGEADLPAGFAAAFETWRNDPRG
                     LSVFTVAFEGSLETLIAKQMADIVGVAA"
     gene            complement(16913..17287)
                     /locus_tag="VF226_22075"
     CDS             complement(16913..17287)
                     /locus_tag="VF226_22075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076736.1"
                     /translation="MGILRQPYRDPAGPANTRRAAITWSVVGFALATVFWSLAGYGPG
                     LGVLARGAAPEYTGSITTAPRKLADRLPAVTRSDSGRLVKTNCTALVLDRADQLTRPA
                     DCLADAPELEYAGTSGRQDRLE"
     gene            complement(17423..18160)
                     /locus_tag="VF226_22080"
     CDS             complement(17423..18160)
                     /locus_tag="VF226_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HEX7076737.1"
                     /translation="MKAAIRSLRPSILLGLSLALFMTSPGFAQRSRAPEHRAGAFDYY
                     TLVLSWSPTYCASLAANDNDPQCRRTARPYAFVLHGLWPQYERGYPEYCPTKERPFVP
                     QRIIDRMLDIMPSPRLVIHEYRKHGTCTGLDPAGYYDLSRRLYEKISIPRRFRSPAAP
                     FFVSPQEVADSFLEANPGLKLEMLAVDCRGSGNRLREVRICFSREGEFRRCGSNERRS
                     SCRANRMYVPPVRAGSGRSMLPGSRAL"
     gene            complement(18193..19590)
                     /locus_tag="VF226_22085"
     CDS             complement(18193..19590)
                     /locus_tag="VF226_22085"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX7076738.1"
                     /translation="MADQLSAVLDALDRSQDAALERLFAFLRIPSISTDPAYKDQCRA
                     AAEWCAAQLREIGFEASVRATTGHPIVVGHDRKEIPEGMPHVLFYGHYDVQPPDPLEL
                     WTAPPFEPRLAEDSRNGTVIVARGAEDNKGQLMTFLEAARAWKQVAGALPVAVTVLLE
                     GEEECGSPSLPGFLEKHAQEVTCDFALVCDTTQWDKDTPGITTMLRGLAGTEVVVAGA
                     SRDLHSGLYGGPAANPIRVLVHVLAALHDDDGRVTVPGFYDGVADVPKSQAELWSRLG
                     FDEKSFLGEVGLSVPAGEKGFTALEQLWARPTLEFNGITGGYQGPGTKTIIPSRASAK
                     ITCRLVAGQDPEKIIASLQSYLAQRIPPDCKVEFGSVHGNAAIAFDPNAPNFQAAAEA
                     LAEEWQRPTALMGSGASIPIVTTFKNQLGMDSLLIGFGTDDDCIHSPNEKYNLTSFRK
                     GARSWARILQRLAST"
     gene            19766..22489
                     /gene="ppdK"
                     /locus_tag="VF226_22090"
     CDS             19766..22489
                     /gene="ppdK"
                     /locus_tag="VF226_22090"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX7076739.1"
                     /translation="MSDSRSESAKQHKWVYLFGAGRSDGSAEMRNLLGGKGANLAEMA
                     SLGLPVPPGFTISTDVCTYYYQNGRTLPEGLDAQVESALGIIGDAVGAVFGDARNPLL
                     VSVRSGARTSMPGMMDTILNLGLNDQTVEGLAARSGDARFAYDSYRRFIQMYSDVVLG
                     VDHGVFEDILESHKNLNGFSTDTELAAEDWQEVIAAYKEAVERETGQPFPQDLHTQLW
                     GAIGAVFGSWQNARAITYRRLHQIPDSWGTAVTVQAMVFGNMGESSATGVAFTRNPST
                     GAKEIYGEFLVNAQGEDVVAGIRTPQNISEKARIEAGDSAPSLEALMPETFAQLKDIF
                     ARLEARYRDMQDVEFTIQNGKLWMLQTRSGKRTAKAALKIAVDLAHEGVISREEAVMR
                     IDASTLDQLLHPTIDPSAHKHIIARGLAASPGAASGEIVFNSDEAEALKAKGREVILV
                     RVETSPEDIHGMHAAAGILTARGGMTSHAAVVARGMGRPCVCGAGQLRIDSKAGQMLA
                     PGGKVFRAGDIITIDGSTGEVLEGKVRMRQPELSGDFATLMAWADAVRKLGVRANADT
                     PRDAQQARQFGAEGIGLCRTEHMFFEADRILAMREMICAEDEEGRRHALAKILPMQRA
                     DFEALFEIMAGLPVTIRLLDPPLHEFLPREEKEQTAVAEALGVPVSRIRTRVTELHEF
                     NPMLGFRGCRLAIRYPEITEMQARAIFEAAVNAEKRTGKAVIPEIMIPLVAYRKEFDI
                     LRDVIVATAEAVEKETGARLQYQIGTMVELPRSALRADEIAEGHNGAEFFSFGTNDLT
                     QTCLGLSRDDAAPILESYLGRGIFETDPFVSLDRDGVGELVRIGTERGRKVRPDLKIG
                     ICGEHGGDPHSVAFCHEVGLDYVSCSPFRVPVARLAAAQAAIAGRVTPKEG"
     gene            22629..23018
                     /locus_tag="VF226_22095"
     CDS             22629..23018
                     /locus_tag="VF226_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7076740.1"
                     /translation="MNLITHEKQEVVDWRPGVATRMLVSAVTGSAQLCVFEQYCDPGL
                     GAPTHLHAVEEVLTVVAGKAEIWVGGDKIQAGAGHSVIIPAGVKHGFRNISENEILHM
                     RATLASPIFEASYDDHREQSRRYVPQD"
     gene            23288..24049
                     /locus_tag="VF226_22100"
     CDS             23288..24049
                     /locus_tag="VF226_22100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7076741.1"
                     /translation="MRLPGKIAIITGAGSGIGHETAKLFASEGATVVVADRNRDAAER
                     VAAEIVNNGGKGSAARVDVTREAEVKSLIEGVAAEHGRLDILVNNAGYGFAGTVVTTT
                     EAEWDALMAVNVKGVFFGCKYAIPIMEKQGGGAIVNTASTVAAVGITDRAAYVASKGA
                     VAALTRAMALDHVSANIRVNCVAPGTIESPYFSEIFAKAPNPDELRRSLEQRQAMNRL
                     GKPIEIARAILFLASDESSFCTGTMLVADGGWTAR"
     gene            24085..24855
                     /locus_tag="VF226_22105"
     CDS             24085..24855
                     /locus_tag="VF226_22105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7076742.1"
                     /translation="MYGLEGRKAIVTGAAQGIGRAIAERLVAEGCDVGIFDRDAEGAR
                     ATADALRTGTRRIAVASGDVSVKADVERGMAFLKAELGPIDILVNNAGICPIGKLLEM
                     PEEQWHATFGVNVNGLFYISRAVVPDMIARRRGAVINLASWMGKSGVASYGAYCASKF
                     AIVALTQTLAVEIGEHGVRVNAVAPGLIVETRMREESEVHRRAMGLSTAEDRAKTIPL
                     RRAGLPRDIANAVAFLASDEADYITGETISVTGGSWND"
     gene            24917..26680
                     /locus_tag="VF226_22110"
     CDS             24917..26680
                     /locus_tag="VF226_22110"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX7076743.1"
                     /translation="MAKTLPRLFSPGSIGPLTLKNRVIMPSMTTRRADAEGFVTEAGI
                     AYYKARAKGGVGLITVEMASPERAGKHRHFELGIYDDRFLPGLRRLVDVIHEEGAKAS
                     IQLGHGGGHTRIDISGEKPIAPSAVPHSVQEGHTEVIIPEEMTISRIAQTTEAFADAA
                     RRAAAAGFDAVEIHAAHGYLISQFLSPKENQRQDRYGGSLENRARFALEVTTAVKKAV
                     PDLAVIFRMNGDDFFEGGMTADEAVEVAKWAADAGADAIHVTGGHYRSQPTAAIMIPP
                     MSTPPTPFLGFAEAVKRRVRVPVIAVGRFGNPSTAISVVEEGRVDFIALGRPLLADPD
                     WVNKAAEGRQVRLCLACNTCVDGMRTGRQLHCLVNSRTGRELALSDRPPRRNGERIAV
                     IGAGPAGLTYAALMAEANEVTVFEKEDEVGGAFRYAGLAPLFQDVEANPDSLLAYVEG
                     LRTECEEKGVEFRMGCDALMDPKLLESFDHVVIATGARYRWGLGGVIKRLLVSGRVRR
                     EPLARFARSDRFRNWFYYRLRFGTGRDAIGRLSGSRSVEAIGDAVNAGKSEAAIMSAY
                     AAAFGPLDETAEEARTHSASG"
     gene            26698..27414
                     /locus_tag="VF226_22115"
     CDS             26698..27414
                     /locus_tag="VF226_22115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7076744.1"
                     /translation="MKERIKEIAVELLVRDGYQGFRFRDLADRLGITRANIHYYYGSK
                     LNLAEEVIVDYVRATLAAWEENWRSDKTFEQKIRGMMESNRQRYLRFNPTGTTGHPWS
                     LIGRMRMERDVIGPEARAALVEFGVVLEDLVVKAIEKAIQEGELSPEIPIRDIALQLV
                     AIANSAGPITQDGGSFDRLEQLYLSFARIVDHAYGRRPGRKQGRSEVAKAAKRKRPSQ
                     PRSGATVGTSGEIEQKQSAR"
     gene            complement(27411..27623)
                     /locus_tag="VF226_22120"
     CDS             complement(27411..27623)
                     /locus_tag="VF226_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076745.1"
                     /translation="MIQVIVYWGLVAFGSMVIGGLLAGLKKRDYSFWMAWSFLFPPAI
                     VVLLLLPPLPHRPRRRTLDEEDAAEF"
     gene            complement(27740..28624)
                     /gene="nadC"
                     /locus_tag="VF226_22125"
     CDS             complement(27740..28624)
                     /gene="nadC"
                     /locus_tag="VF226_22125"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7076746.1"
                     /translation="MHMIPRSAFDLRLPEPLIASTVAGALAEDLGLAGDVTTDAVIAP
                     EATASGVFAARKPGIVAGLQVAAATFRHLDPEVRFDVLVPDGGTVEPGKIIARVSGNA
                     RALLTAERVALNFLGRLSGIATLTRRYVDAVAGTRAQIVDTRKTTPGLRAFEKYAVRA
                     GGGMNHRIGLFDAVLIKDNHIAVAGGVGPAVAAARARVGHMVKIEVEVDTLDQLSEAL
                     QHRIDAVLLDNMTPDQLAEAVRIVAGRAITEASGGVNLDTVRAIAEAGVDLISVGALT
                     HSATVLDIGLDIEVGTMA"
     gene            complement(28624..30249)
                     /locus_tag="VF226_22130"
     CDS             complement(28624..30249)
                     /locus_tag="VF226_22130"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX7076747.1"
                     /translation="MIKGGQVNQPAAGGDIVIIGAGLAGLFTALKLAPAPVTLISPAP
                     LGEGTSSAWAQGGIAAAVGEGDTPEAHAADTIRAGAGIVDEHVAHIVACEARDRIRDL
                     LAYGVPFDRDLEGHFVLSREAAHSAGRVVRVSGDRAGRAIMQALIHWVRQAPSITVLE
                     NYTALSLNKTGDRVTGVNLARADDPSAIYVLDGARAVVLATGGIGGLYAVTTNPPHAR
                     GEGIAIAARAGATIADAEFVQFHPTALDVNLDPAPLASEALRGEGAVLINGAGERFML
                     HQHPDAELAPRDVVARAVFREIAAGRGAFLDCREAIGARFPETFPTIFEYCRAAGLDP
                     VTQPLPIAPAEHYHMGGVRTDALGRTSVPGLWAAGEVACTGLHGANRLASNSLLEALV
                     FGARVAADIGRLERGRTTRVAVTRTRTTHLPDFAAREEAIRVIRTAMSRHVGVERNAE
                     GLTKALAILESVGEAADGDPVIENATIAARFITECALRRKESRGGHYRSDYPQADPQW
                     LRRSAITLAGLHLRSALKAGELASVIGATTDRI"
     gene            complement(30307..31365)
                     /gene="nadA"
                     /locus_tag="VF226_22135"
     CDS             complement(30307..31365)
                     /gene="nadA"
                     /locus_tag="VF226_22135"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7076748.1"
                     /translation="MATVQTEIRAPAAKSRDALPMPDLKFTPELAREMEPLYAKVSKV
                     VTPLEWPQFAPIIKAINDLKKQRNAVILAHNYMTPEIFHCVGDIRGDSLQLAKEAART
                     DAQVIVQAGVHFMAETSKLLNPSKTVLIPDLRAGCSLASSITAEDVRMLREAYPGLPV
                     VTYVNTSAEVKAESDICCTSANAVKVIESLGVPRVIMIPDEYLAKYVQTQTNVEIITW
                     KGHCEVHERFTAEELEAFRAADPGLKIIAHPECPPDVLKAADFAGSTSGMINWVKTHK
                     PAKVVLVTECSMSSNVAAEAPTTEFVRPCNLCPHMKRITLQNILESLLYMRHEVTVDP
                     LVAERARRSVERMINLTN"
     gene            complement(31723..32760)
                     /locus_tag="VF226_22140"
     CDS             complement(31723..32760)
                     /locus_tag="VF226_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX7076749.1"
                     /translation="MTFEAHLKQATRPSARASEPRERHEVAAVEIGLNAAILAVRMDE
                     PVVLVVRSHEGDQAESLPFGPFSPLEHRTLEIGLRSWVAEQTGLELGYVEQLYTFGDR
                     GRHAQPGDTGPHVVSIGYLALTRPGPGGDSDRWEWRSWYDYFPWEDWRGGKPAILTEV
                     IEPKLMAWADRPPLSSSGRPIQRTDRLRMCFGLYGGTWDEEKVLERFELLYDAGLVEE
                     ARRDGREAALTWGNLPRLGAPMRFDHRRILATAIGRIRAKIKYRPVVFELMEDEFTLF
                     ELQKTVEAILGPHLHKQNFRRLVESAGLVEPTGEVRAKTGGRPARLYRFRREVLLERP
                     APGVRVKPGRV"
     gene            complement(32849..33403)
                     /locus_tag="VF226_22145"
     CDS             complement(32849..33403)
                     /locus_tag="VF226_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HEX7076750.1"
                     /translation="MAAQKIPSGSGPVGLHVCPLSFVPNVVAETGARHLITLINQHTM
                     LETPAGIDAANHLRIAVNDIVAPQEGLVHPCEAHVEALLRYVRDWNRQGPLVVHCWAG
                     ISRSTAAAFITLCAINPETPEWLVAQRLRRASPTACPNNLLIRLADDMLGRRGRMLHA
                     IKEMGPPEPATEGRPFMLPLHFDP"
     gene            complement(33563..34087)
                     /locus_tag="VF226_22150"
     CDS             complement(33563..34087)
                     /locus_tag="VF226_22150"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7076751.1"
                     /translation="MGWSRKTMGWETRRLSGEGSTSSGRPAADHIRDDVQSRLADDPG
                     IDASDIEVDVQGAHVILRGSVPSELTKQMAEVIAFEAEGVTRVENALVVRARRRRDAP
                     PPPTRHERENPIEPGTDSYLEASVSPQPSSPDTPFDPNNRPGIGTGGAPADTSGSPVD
                     SGVNLGSPHRNPKP"
     gene            34219..34983
                     /locus_tag="VF226_22155"
     CDS             34219..34983
                     /locus_tag="VF226_22155"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7076752.1"
                     /translation="MIISEVMTREVEVISPDASLREAARKMDELNVGVLPVCDGQRLV
                     GMITDRDITVRATSIGESPDVTRVDEVMSEEVWWCYEDEDVEDVARRMGEKQIRRMPV
                     IDHDHMLVGIVALGDLATDRAPGTQRALRKISEPSEPDLSGTLSDRRADPAHFALLED
                     VAEERPSRTDDVIAEDVFRGLDAAEEIDVAEIDIYVDHGVVSLNGTVDNYAAQELATK
                     IAEGVEGVTRVDNNLRIRTDAGVLPDVRFKGLRTAK"
     gene            complement(34995..35648)
                     /locus_tag="VF226_22160"
     CDS             complement(34995..35648)
                     /locus_tag="VF226_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076753.1"
                     /translation="MRDPPPLDGGRGRAIPLWLKLAYTAFMALLVPIYWHHYGPANFL
                     FFSDLALFFVLAAIWLESPLLASMPAVGTLATQALWIADFVAVAVGGRITGMTGYMFD
                     SGYPFYLRALSLFHIWLPALLLFLVWRLGYQARAFWAWTVLAWGVLLISYFLLPPPSP
                     DPGGTARNINYVYGFSDTEPQTIMPQWMWLLMLMAGYPLLIFAPVHFALRRWAPQVS"
     gene            complement(35663..36505)
                     /locus_tag="VF226_22165"
     CDS             complement(35663..36505)
                     /locus_tag="VF226_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX7076754.1"
                     /translation="MRFEGTDSYVATKDLTVAVNAAITLQRPLLVKGEPGTGKTMLAI
                     EVAKSLGVPIIEWHIKSTTKAQQGLYEYDAVSRLRDSQLGDERVKDIRNYIRRGKLWE
                     AFEMEVRPVLLIDEIDKADIEFPNDLLQELDRMEFFVYETGETIRARNRPIVIITSNN
                     EKELPDAFLRRCFFHYIKFPDPETMRSIVEVHFPGLKGRLVSEALKIFYDIREVPGLK
                     KKPSTSELLDWIKLLLNEDISPETLRENDPRKLIPPLHGALLKNEQDVHLFERLAFMT
                     RRQQ"
     gene            37203..37568
                     /gene="dksA"
                     /locus_tag="VF226_22170"
     CDS             37203..37568
                     /gene="dksA"
                     /locus_tag="VF226_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEX7076755.1"
                     /translation="MNERQRAYFRRKLLAWKEEIILQTRETLMGLHEDSTQHADLADR
                     ATSETDRALELRARDRHRKLIAKIDAALARIEDGTYGYCEETGEPIGLRRLDARPIAT
                     LSLEAQERHERRERVYRED"
     gene            complement(37578..40070)
                     /locus_tag="VF226_22175"
     CDS             complement(37578..40070)
                     /locus_tag="VF226_22175"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HEX7076756.1"
                     /translation="MVLGLTRLIIVLAGCFGLLTATLAQETVGTGEAAAPTSAQPAAI
                     AAPSFFPAELAQRVARVASDIESAAKGIERVKSREDGLTAQRLELERIEVEAQQVIDA
                     LRPRLAAVKAQVQKLGPPPDKDAAPEAPAIASERSRLTAVQAELEGAVKTVELALVRS
                     RQLVGHVQRLRQALFARDLVRHSGSPLSPATWKAFAAELPRTGRQIARLAAEWWRLAG
                     SNLVGLGLVLAAAVGAYALFKLPLSHIRRRIHESPIALRQYFPRAIDAFWLAAGLAFP
                     PLAAATVGYVGLAWLGLLTVTAGELALSALRAFVVFQIIRALAGAAFSPTASGQPLIA
                     IADASAPKLLFLANLLAGLIALDLLLGDVVRMLYLPIEIGAVQATATSLAFATLLIAF
                     TRVPLAGREAALLEAPSRSRPRWLKIPIALAALTIVATTLAGYVALGKFITTQVMLIG
                     GLGLFILSVHLVIRRVAVVMTDGERPMGRILETRLGLDHDRTSYLTRLLVLLVELLLL
                     LAALPLLLLTWGYTREDFLDWLRLGIVGIEIGQFQISLGRILLSFALFFVLLFATRVV
                     QRWLDRSVLGPARVDRSIAHSVLMGVGYTGVGLALVIALSYAGLDFTKLAIVAGALSL
                     GIGFGLQAIFNNFVSGIILLIERPIKVGDWIVVNGQEGFVRRINVRATEIETFDRSSL
                     IIPNSELITGTVMNWTHRNLIGRLIVSVRVSYKADPEQVMKILADVVDRSPSLLREPA
                     PLIVFEDFGESAMMFSVRAFVPDVMRRLTVQSELRLAIAKAFRDAGIEIPYRQHDIHL
                     RDLDGVKQAFARALEARRREQEVEAGDTSPAS"
     gene            complement(40155..>40620)
                     /locus_tag="VF226_22180"
     CDS             complement(40155..>40620)
                     /locus_tag="VF226_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEX7076757.1"
                     /translation="PSRRHENVHASNQQIERSAGAPASSQMEILYWLLFVIFIAALLV
                     VSGLLLRGYFTTGSPSSALKDALSGNFFGPRPPKRLEVVDQANVDGRRKLLLIRRDGI
                     EHLIMTGGPVDVVIETGIEPPEAPSSQSPMAEPAEPMFQRPARSLQEAVGKA"
BASE COUNT         7291 a        12718 c        13384 g         7227 t
ORIGIN      
        1 ccgagcttgc cttcggctgc ctgcggcgga ccgccgacga cgggcgcatc gatgaaggtg
       61 acgcccttgg cctttgcctc cgccgcgagc atttccgagg tctcgggctt cgacgtcgtg
      121 acgtcgatga tcatcttgcc cgcactcatt gcgggcagca tctcgcggac gagctcggcg
      181 accgaatcgg cgctgttgac gcagatcatc acgacgtccg cgtccgccgc cagctcgcgg
      241 aggcttttcg cttccgccgc gccacggccg agcagatcgt cgatgcgtgc gcggttccgg
      301 tgggcaatga cggtgagtgg gaaaccagcc ttgatcaggt tgaggcagat gccgtggccc
      361 atcatgccga cgccgatgaa gccgatcttc tgctgcggac cgttggacat cgaagccctc
      421 ccactggcgg ccggagatgg tgggcggtga gaggatcgaa ctcccgacat cttcggtgta
      481 aacgaagcgc tctaccactg agctaaccgc ccgcgcccga ggcttctagc gcgacgggcg
      541 ccgcttgaaa agcgctccgc tggattgctc tgtcctttac cgccgcaggc atgcctcgaa
      601 gaacgccatg ttggggcagt attccgacgg atgctcggct ccgggcacgc cgtcgagcca
      661 tcgccggcat tcgctggtcg accggcacac gaggcagttc gtgcgcgcct gcgcgaatga
      721 agcgccgctc tggtggcgcg ctgcggcgag cggatcgacg cccaaccgct ccatcatctc
      781 gcccatgagc tgggcctgcc gctcgacgtg gcggcacatc gaccaaggca taatcgatcc
      841 tccatggtcc ccgccctgat ccggcgctcg ggcgcgccgt tgtcgagttc agattcctgt
      901 gagcggaagg cctgctggtt gatttggatc aagggcgccc cgcggcaaaa gcgccatggt
      961 gggcgactcc gcttggcgac tggttctgtg tgcgggatgg ggtagcattc tcccttgagt
     1021 cgatctcgta agcaggagga accagtatta tgtctgacca gcctggcgcc ccacggttcg
     1081 atacgcttgc catccatgcg ggtgccgctc ccgacccgtc caccggcgcg cgcgtgacgc
     1141 cgatctacca aacgacctcg tacgtcttca acgacgccga ccatgccgcc gctctgttcg
     1201 ggctggaggt cttcggcaac atctacaccc gcatcatgaa cccgacccag gccgtgctgg
     1261 agtcgcgggt cgccgcgctc gaaggcggca ctgcggcgct ggccgtcgct tccggccacg
     1321 cggcgcagtt cctggcgttc cacacgctgc tggagccggg cgacgagttc gtggcgggcc
     1381 ggcagcttta cggcggctcg gtcaaccagt tcaatcactc gttcaagaag ttcaattgga
     1441 acgtgatctg ggcggactcc accaatccgg agagctttgc gcgtgccatc acacctaata
     1501 cgaaggcgat cttcgtcgaa tcgatcgcca atccaggcgg catcgtcgtc gatctgccgg
     1561 ccattgccgc gatcgcgaag aaggccggcg tgccgttcat cgtcgacaac acgttcgcga
     1621 cgccttacct gtgccggccg aaggagcatg gcgcggacat cgtcgtgcac tcgctgacga
     1681 aattcatggg cggccatggc aactcgatcg gcggcatcat cgtggattgc ggcaccttcg
     1741 attggctggg atcgaagcat cgctacaaga ccttggtcga tccgaacccg tcctacaacg
     1801 gcatgaagct cgccgagacc ttcggcaact ttgccttcgc catcgcagcg cgcgtcatgg
     1861 gtctgcgtga cctcggcccg gccatcgcgc cgatgaacgc gttcttgatt ttgacgggca
     1921 tcgagacgct gccgctgcgc atggcgcggc attgcgagaa cgcccaggcc gtcgcggaat
     1981 atctcgtcaa gcatcccaag gtggcgtggg tcaattatgc agggttgcct gacaacccga
     2041 gctatgaggt gcacaagcgg gtttgcccga agggcgttgg ttccgtgttc acgttcgggc
     2101 tcaagggcgg ctacgacgcg ggcgtcaagc tggtcgccag cctgaagctc ttctcgcacc
     2161 tcgccaacgt cggcgacacg cgcagcctcg tcattcaccc ggcgtcgacg acgcaccggc
     2221 agctgacgga cgagcagcgg attgcggcag gcgccgggcc cgacgtggtg cgcctctcgg
     2281 tcggcctcga agacaaggcc gatatcatcg ccgatctgga tcaggcgctg gcgcaggtgt
     2341 gagtgagccg atccagcaat cttaggctcc ccgaaatttc aactaactgg ctccgcggat
     2401 tgcccgcgga gctgattttt ccggagccaa agtccactct tccgatataa tccgttttga
     2461 aataatctgc ctcgccatca atgcaacgag ggggggtgct gtgaagcatt ttggcgtctt
     2521 tgtattgtcc gtttgcctgt taagcctttt ccaagagcca gtgtcggcag aaaccaatcc
     2581 gcagttcacc aggatcgcgc agccaggaat ttgcgctgtg tgcaggccgt tcggccgcaa
     2641 atgctgtgcg ctccgggctt cgatcgactc atgtgtgaag tgcagcctta gctacggcta
     2701 cgagcgcgct ggtgcaatca ggtggtgcac catcaaccag ccccgttgcc ggtagacgaa
     2761 tcgcgactac gcccgacgca gagcgatctt caccctttga ccttcacgta gcttcccggc
     2821 gcatcctcga tgacgccgag caccgcaccc ggcttacggg cgggaatgcg cttgccggaa
     2881 tgcttggcca gccatttggc ccagtgcggc caccaggagc ccggcgtttc cttggcgttc
     2941 gcgatccact cttcgagagt ctcggcgggc ttcttgttcg tccagaattg gtacttcacc
     3001 cgcttcgggt ccggcggatt gatgacgccg gcaatatggc ctgagccagc cagcacgaac
     3061 tccaccggcc caccgaacag cttcgatccg acgaagacgg acttcgccgg tgcgatatga
     3121 tcttcccgag tggcgagctc atagatcggc agcttgacct tgccgagatc gagccgtgtt
     3181 ccggcgatct tcatctgccc cttcgcgagc ttgttctcgt gatagaactc gcgcaggtag
     3241 aacctgtgat tggccgccgg catccgcgtc gaatcctggt tccagtagag caggtcgaac
     3301 gggaagggct tcttgccaag caggtagttg ttgacgatgt agggccagat caggtcccgg
     3361 ggccggagca tgttgaacac ggaggccatg cgagacccat cgagatagcc gcgctcggcc
     3421 atcatctcct cgagcgccgt gagctgtgca tcgtcgatga aaaccaaaag gtcgccggcg
     3481 tggttgaagt ccacctgggc ggcaaggaat gtcgccgagc ggtagagctc ttcgccacgg
     3541 gcagcaagat aggcgagcgt cgtggcgagc agcgttccgc ccacgcagta gccgagcacg
     3601 ttgatcttgt cctggccggt ctcgcgcctg acggcatggg tagccgccag cgcgccctcg
     3661 atcatgtagt cctcgaacgt cttgtccttc agccgttcgt ccggattgac ccaggaaaca
     3721 acgaaaacgg tgaaaccctg atcgacggcg aatttgatga acgacttcgg ctgcgtcagg
     3781 tcgaggatgt agaacttgtt gatccacggc gggatgatga gcagcggtct ctcgtaaacc
     3841 gtctccgtcg tcggcgtata ctggatgagc tggaagagat cgttctggaa gacgaccttg
     3901 cccggcgtca ctgccaggtt gcggcccact tcgaacgcct cgaggtcggt ctgccggatc
     3961 ttcagcaggt cgcctgagct ctccatgtcc tcggccagat tttgcaggcc ctcgatcagg
     4021 ttccggccgc tggtggcgag cgtttcgcgc aagacttcgg gattggtggc agggaaattc
     4081 gacggcgaca gcgcgctcag caattgctcg aggtagaact cggccttcat ccgcgtgcgc
     4141 tcatcgacgc cgtccgtctt ttgcaggacg ttttcggccc actgcgccgt cagcaggtac
     4201 atttgcttca cgaagtcgaa atatgggctg gcggaccatt ccgtgtcctt gaaccggttg
     4261 tcgcccgccg ccggcttggc tacgggttcg acctcctcgc cgagcgcccg gcgaatcgcc
     4321 gagtgccaaa gatcgagata gccgcgcacc agctcgccct gtgcttccgc aaggcgcgca
     4381 gggtcgccaa accagtagcg agcgatttcg cccatggctt tggccgcctc ggccatctcg
     4441 ttgacgccgg agtaggggcc gagcctcgtg tcggcgcgct cggcgagctg agccatcacc
     4501 ttgccacctt gctcgaagag cttcagcagg ttcagcgcca gttcctcggg gttgccaagc
     4561 ttgtaggccg ttgcatccga ccgggcatca gggttcggct tctgcatggg ttgcctgcac
     4621 gtttggggct tgccagaaat ggccggaaat tcacggacgg ctggaagcca acgtgtccga
     4681 atcatcacaa tttctttgtg cttaaagata gtgtcacgat cgcgcctgtt ccagcggccc
     4741 ggcgcaagct tctgaatcgg gtaagctacg cgaatgaggg attgcgactt caggctcgcg
     4801 cgtcgcgcgc cgaagctgca cgagaggcgg ctccagaagg ccgttccggc agcagccggc
     4861 atcgtgctgc tcgcgattgg tctggccagt tccctcgcca gctgctcgcc aggcaactcg
     4921 acccggcttc ccgagctgtc gtcgatacca cgcaagctgc tctcggctga agagcagaag
     4981 aaggtcgtcg acgagctcca gagacgccgc gacacccacc gcgaggaggc gatcaaggag
     5041 atcaagagcg cgcgcgccgc tgacccgtca ggatgagaaa tcgcgcctta cgagctcgat
     5101 ccggccctcc cgacgctgga cgagcagaag caaacgatcg taaacacgag cgaccgaaca
     5161 ctgtctgggc tgatcaacaa gtgagatgat cccgtgaccg acgagttcca tcgcatcaag
     5221 cgcctgccgc cctatgtctt tgccgaggtc aatcgcctga aggcagacgc gcgagccgcc
     5281 ggagcggaca tcatcgatct cggcatgggc aatccggaca tgccgacgcc gaagcacatc
     5341 gtcgacaagc tcatcgagac ggtgggcaag ccgcgcacga accgctactc cgcctccaag
     5401 ggcattccag ggctgaggcg cgcgcaggct gcctattacg agcggcgctt cggcgtgaag
     5461 ctcaatcccg agacgcaagt ggtggcgacg ctcggctcca aggagggatt cgccaacatg
     5521 gcccaggcga tcaccgcgcc gggcgacgtc attctggtcc cgaacccgac ctatccgatc
     5581 cacgagttcg gcttcatcat ctccggagca tctatccgcc acatcccggc gagcaacgga
     5641 ccggagttcc tgcgcggcgt cgaacgggca gccaagtact cgatccccaa gccgctggcg
     5701 atggtgctga gctatccgtc gaacccgacg gcgatgaccg cggacctgga cttctatgcc
     5761 gaggccgtgc agctcgccaa gcgcctgggg ctcatcatcc tgtcggatgt cgcctacgcg
     5821 gagatctatt tcgacgacaa tccgccgcca tcggtgttgc aggttcctgg cgcgatggac
     5881 gtcgccgtcg aattcacctc gctttccaag acctacagca tgcccggctg gcgcatcggc
     5941 tttgccgtcg gcaacgagcg gctgatcgcg gcactggcgc gggtgaagtc ctacctcgac
     6001 tacggcgcct tcacgccgat tcaggtggcg gccgcggcgg cgctcaatgg cccgcaggac
     6061 tgcgtggaag agatccggca gatctacaag agccgccgtg atacgctcgt tgagagtttc
     6121 tcgagggcag gctggaacat tcccgccccg cccgccacca tgttcgcctg ggcgccgatc
     6181 ccggagccat accgcgacat cggctcggtt gagttcgcca agctattgat agaaaaagcg
     6241 gacattgccg tttcgcctgg cctcggcttc ggcgagtacg gcgaaggcta cgtgcgcatc
     6301 gcgctggtgg agaatgagca ccgcattcgc caagcggcgc gcaacctcag gagatttctg
     6361 tctcaaccgc ctgagttgat gcacaacgtc attcccatgc agcagaaggc gtcgcgttag
     6421 gcccatacat gtcaccacct ttgaaaatcg gcgttgccgg cctcggcacc gtcggcaccg
     6481 gtctccttca agtgctcgag gtccacgggg gccggctcgc cgcaacgctc ggccgcgaga
     6541 tcagggtcgt tgccgtgtcg gcacgctccc gcgacaaggc gagaagggcc cggttcgagc
     6601 acttccgatg ggtggccgat gccgtcgagc tcgcaaccga tcccgagatc gacgtcttcg
     6661 tggagctgat cggaggcgac agcggggttg ccgccgaggc cgtcgaggct gccctccggg
     6721 ccggcaagca cgtggtgacg gccaacaagg ccttgttggc gcagcacggc atccgcctcg
     6781 cccgtttggc ggaggagaaa ggcgttgcgc tcaacttcga ggcggcggtg gccggcggca
     6841 tccccgtcat caagacgatc cgtgaggcgt tgctcggcaa cgagatcagc cgcgtctacg
     6901 gcatcctgaa cggcacctgc aactacatcc tctccacgat ggagaaggag ggccgcccct
     6961 tcgccgaggt gctgaaggag gcccaggagc ggggctatgc agaagccgac ccgaccttcg
     7021 acatcggcgg cttcgatacg gctcacaagc tgtcgctgct gacggccttg gccttcggaa
     7081 cagagctggc gttcgaggat atccacgtcg agggcatcga gcacatcacg ccggcggaca
     7141 tggaggcggc cgacgatctc ggctatcgca tcaagctgct gggcgtggcg ttgcgcacgc
     7201 cgagcggcat cgagcagcgg gtgcatccgg cgatggtgcc caagcactcg gcgatcgcgg
     7261 aggtttcggg tgtcacgaat tgcgtcgcca tcgacggcga cttcgcaggc gacctgctcc
     7321 tggtcggcgc gggtgcgggc gcgaagccga ccgcatcggc tgtcgccagc gacatcggcg
     7381 acatcgcccg cgggctggtg ctgccgccgt tcctcacgcc agcaaagcag ctgaagccct
     7441 atgcccgggc catgctggac cggcatcagg gcgcctacta cgtgcggctc accgttcacg
     7501 accggccagg caccatggct gccatcgccg cccggatggc cgagcaggac gtctctatcg
     7561 aaagcatcgt gcagcgccgc ccccgctccg ccttgccggg caccggggcg cggccggctc
     7621 cgggcacgct gacgcccgtc gtgctcatca cgcacgagac gacggaggag gcgatgcgca
     7681 aggcccttgc cgcgatcgag cgcgacggca aggtgtccga gatgccgcag atgatccgga
     7741 tcgaaaggct ttagcgggtg gcgggctcgg gacagccggg agatggagga agaggcccat
     7801 gttggttgac cttgccgaag gtttggatcg tgtgttcgtc ctcgaggtgg ggcgcataac
     7861 ggaggccgcg gccattgccg ccgcaaggct tcgtggccgt ggcaacgaga aggcggccga
     7921 caaggcggcg gtcgatgcca tgcggcgcga gctcaatcgc gttcacatcc gtggccgggt
     7981 ggtgatcggc gagggcgaga tggacgaggc gccgatgctg tatatcggcg aggaggtcgg
     8041 catcggcgac gggccggagg tcgatatcgc cgtcgacccg ctcgagggca cgaccatctg
     8101 cgccaagtcg atgccgaacg cgctggccgt gctggccatc gcagaacgcg gtgggctgct
     8161 tcatgcgccg gacatgtata tgaacaagat cgccatcggg cccggctatc ccgagggcgt
     8221 catcgatctc gatgcccccc cgatcgataa tctcaatgcg ctggcgaagg ccaaaggggt
     8281 gccgatcggc gagatcacgg cgtgcatcct cgacaggccg cgccatgccg agcttatcaa
     8341 ggcggtgcgc gaggcgggcg cagccgtgca gctgatcccg gacggagaca tcgccggtgt
     8401 catctggacg accgatccgg aggaaaccgg catcgacatc tacctgggct cgggcggcgc
     8461 gccggaaggc gtgctggctg ccgccgcgct gcgctgcatc ggcggccaga tgcagggccg
     8521 cctgatgccg ctcaaggacg aggagcggga gcgcgccgcc aagatgggcg cggacgtcga
     8581 ccgcaagtac agtctggagg acatggcctc cggcgacgtc atcttttcgg cgaccggcgt
     8641 gaccgacggc tcgcttctcg acggcgttca tttccgcggc gatttcgcgg aaacggaaac
     8701 cgtcatcatg cgcgccaaaa cgggcaccgt ccgccgcatc aagaccacgt tccgcaacat
     8761 cggcacgaag ctggccgggc tttgagtccg gactttcgaa tctagtcccg gaaggcttcg
     8821 gaggccgcgc gaattcggtc gagctccagg ccgaacgagg ccgccatctc gcttgtcaga
     8881 ggtgcgccga aggcgttgac ggctcgcgtg aattcgcggc gccagtcggc gaaggggcct
     8941 tgcgtttcgg aatggttttc cgaggtgttg atgagatagg tgttccgcga gggcaattgg
     9001 acgagcggaa cgccgaaatc ccttgccagt tcgatccacc ggtagtgctc gtggagaacg
     9061 cggtgagggt cccggcgcag gggatcgcga tgcccaggtc tgagttgcgc gacggtgctt
     9121 gtcccgcaga gctggtgaaa accgcccgcg aaaatgcggg ggtccggtaa tttcgcggtg
     9181 cgcagtgtcc ggaagtcggt ggcaaacccg gcagtcgtta cgccgccgat atggtcaggc
     9241 ccgatgaggg tccgtgcgct ctccacgaga tgcttgtcga tccagtcgtc ggcatcgacg
     9301 aacatcagca gcccgccgcc gcgcgccatg accagcgcgt tgatggcgtg gaccttccgg
     9361 ccgcggtcga gattgtccga acgctgcggt tccgctggcc agtccgcctg gagaaattcg
     9421 atgcgcgggt catcggggaa attggcaggc cggtcgtggc cggcgacgac gatgcggaaa
     9481 tcctgatcgg actgggccag aatggaatcg agcgtcagct tgaagaggca ctcgatcagc
     9541 gcccagttcc tcgcaccgag acgcggcaga agcgggatac cgaaagtgaa cgcaatggtc
     9601 gaggacaggg cgggcctcct tcaggtccga tcgcgtgacg ccggacggga atagccggtc
     9661 ggatcgttcg gttcccttgg cgggcgtggc cagcgtggaa cctgctcgta ccagccatgt
     9721 tcacggggcg actggagtgc gagcgaagaa ggacagcccg gatgcgcccc ctgccaggaa
     9781 agcccgactg ctgctggacc gtcgttgcgc ccgagacgag ctatcccccg ctccagggga
     9841 agcatgctgt cgacatcgcc gtcatcggcg gcgggatcgt agggttgacg gccgcgctgc
     9901 agattgcaaa agcggggcgc tccgtcgccg tcgttgaggc attgcgggtt ggcaggcagg
     9961 tcacggggcg ctcgacggcc aagatcacca cgcagcatcg gctcatttac gatcacctga
    10021 tccggacggt cggtcgcgag aaggcgcaac tttacgcgga tgccaatcgc aaaggcgcag
    10081 aattcattga aagctgcatc aacgagttcg ccatcccctg tgacttcgaa cggcggtcgg
    10141 cgatcacctg gatcgaggct gacaaggagg gtcttgccga tatcgaggcg gaagcggagg
    10201 ccgcactcag tctcggtctg cccgccgacg tgctcgatcg cgcgcctttg ccttttgcca
    10261 ccggcggcgg gctccgcttc accgatcagg cgcagttcaa tccggcgctc tattgcatcg
    10321 ggctggcggc agcggtcgtc cgttcgggcg ggcaggtgtt cgagcgatcc cgcgtcattg
    10381 ccgtcgagcg cgaagacctt tggcgcattc ggacggaggc tggtgagatc cgggcggaaa
    10441 gcgtgttcct cgccacaaac cttcccgtcg ccggggaggt cgatttcagt cactacgtgc
    10501 aggcacgctg ccacacggcg gcagctttcc gcatcgcagc cgagcgcgcc ccggacggca
    10561 tgttcctatc ggtcggccat ccgtctcatt ccatccgcgt cggccgggac gacgacgggc
    10621 tcctcctgat cgtgctcggg ccacacttca tcaccggcca ggaaggcaat gtcgcgcggc
    10681 gctttctcga gctggaggac tggacacggg agagattcaa ggtcggagat atggtctggc
    10741 gctggacgaa cgaggattac gacaccgctg accgcattcc gtatgtcggt gcaccctcgg
    10801 caaaggagcc cggcctctac atcgccactg ggttcaacgc ctgggggatt acgaatggga
    10861 cggctgccgg acttctcgtt gccgatcagg tgctgggccg ggaaaatccg tcggcagccc
    10921 tttacgaccc aacgaggccg cttccgaaga gcttccacaa gggtggaaag acccagagca
    10981 aggctgcaga tgtcagcagc atccggctgg gcgaaggtgg cgtcgttcag atcggcgacg
    11041 agaccttcgc cgtccataag accatgagcg gcatcgtgaa cgcgctctcg gcatcctgca
    11101 cccatcaggg atgcaccgtg acctggaaca acgccgacaa gacctgggac tgcccgtgcc
    11161 acggctcgat cttttcggct gacggctcgg tgctccacgg tcctgccgtc gagccgctgc
    11221 cctcccgcaa gctgccgtaa gcggcttttg gtgcggtcga cccgacccga cgcctactcc
    11281 gcggcttcgc gcgcctgccg tgcgtcgtag gcggcgcggg cactggaaac gcgggcatgg
    11341 ttggcgatgg cccactggcc gagggcacgc agtggcacca gcagatcccg acccaaatcg
    11401 gtgagctcat attcgacctt cggcgggatc gtcggataga cggtgcgggt cacgtacccg
    11461 tcgcgctcca gcccgcgaag ggtgacggtc agcatcttct gcgagatgcc gtcgacggcg
    11521 cgcttgatgt cgctgaagcg cttcttgccg gcgcccagca gcacgatgat cagcacgctc
    11581 cacttgtcgc caaccagcga gatcacatcg ttgaccagct ggcattggcc aggaacgtag
    11641 ctctcactcg gttccatcat ggtacctcag tcgcaaaaaa gtgccttctt gcgcaggaaa
    11701 ccacagtctc gcatatgaag ctagtaactt ttctatacgg aggctctaat gaagactgtg
    11761 aagactgtcg cagtcctcgt cggctcgctg cgccgcgagt ccatcaaccg caagttcgct
    11821 gaaagtgtgg gcaagctcgc agccggacgg ctggagttcc ggttcgtgga aatcggtgac
    11881 ctgccgctct acaacgatga tctttgggcc agtccgccgg agtcggtgtt gcgcatgaag
    11941 agggaggtgg aggcggcgga tgccgtgctg ttcgtcacgc cggagtacaa ccgctacttc
    12001 tcgccggcga tcaagaacgc catcgactgg ggtacgcgtc cttggggcga gaattcctgg
    12061 gcaggcaagc cttcggcggt gatcggcacg actaacggtg caacggggac ctcggctgct
    12121 cagaactctc tcaaggcttt gctgaccgtc gtcgagacca ttctcatggg tcagccggag
    12181 gtctatttca cgtacaagcc ggagctgttc gacaaggaca acaatgtcgt cgacgactcg
    12241 acgagggact ttctcaacac ctggatcgac cggttcgctg cgtggatcga gcacaccgga
    12301 cgagcccaca ttcctgcaaa ggccgcctga tccaatcgat ccggctgcgc gaggctgcgg
    12361 ctcccccgcg gttttgttcc tttgacagaa taatacccta tcggggcctc agccccgatc
    12421 cgctgccggg cagagctgga aggcacgggc gaaagcggct tcgctcgacg agaattcgcc
    12481 gatgagcgtt ccgcctggcg cgtgggcgtt gatgtagctc tccgtcatcg tctcttcgaa
    12541 accgtcgagc acgcgcagcg cggtgtactc gacggcgtct tcggcgacgt ggcggatgag
    12601 cattgtcttg gtcccgccat agctcgccat cagcacgcgg caggcactcg gctcggcagc
    12661 tcgcggttct ggcgcgggcg ggcgaatggc gcgagtcggg cgaggcgctg cgagagctgc
    12721 ggctccggcc cgggccgtcg ttgtctgcca ggcgggggcg cgggccgcgg cgtgagtttc
    12781 cttcccagcc gtcgcaatct gatcgccatt gcaataagtc ttgcggaagc gctcggccgt
    12841 aagctcgatc gagcggccgt aggcccggtc cgcagcccac ttgccggcga ggtcctggaa
    12901 ggtgaccggc cgctttctgg aaagcggtgc cgtccaggaa aggatgtcgt cctgctttgc
    12961 tcgcgtacgc tcggccaccg gattcggtac gcgctcgccg gaatagacga ccaggtgctc
    13021 gagttgggct ctgaccccgg ttgccacgtc agggaagctg tttccgggca cgccgccgcc
    13081 ggtcgcaccg atgccagcga agttgttctg gctcggatcg acatcgccgc gcttgccgtt
    13141 gccgcggcgg aaggtgagat agttggtctc gatcaacatt tggaagaacg cgtaatccca
    13201 gcggacaccc agcgcctcgc catagcgctt gtaccagacg gcgatgtcct tgaagcgcgg
    13261 gtcgagatcc ttgttccggg cccgcaaaaa ggccatcagc cgctcaggcg tcgcgcaggc
    13321 cggtacggga ttggcggcgc tgatgcggat ggcgggggcg gcgagggcag cgtcccccgc
    13381 cacggccgtc cccgtcaaga ggcatgcgaa cagggctgca gcacgcgccc atccgcccaa
    13441 cgcgtgaggc ttggctcgga tccgcatcag ccttccggac acagctcgaa ggcacggtcg
    13501 agcgcactca gctgattgga aaactcgccg atggtcttcc cgcctttggc gtaggccgcg
    13561 atgtaggctt cggtctcgcg cttctcgcgg ccctcgttga cgtcgagcac ggtgtagttc
    13621 accaggccgt cgctcacagc cttgatgatg accgacttct ggccgccgta gctggccgtc
    13681 cagacgcggc acttcggagc cgcactttcc tttgcggccg aagccgcggc tgcccccgcg
    13741 gacgccgtct gcaatttcgc ggttccgtca actttggagc tgccactctg ccacgcctcg
    13801 gagatggccg tgttctggtt ggccatgctc gacgtccctg cggtcttggc ggcgatcgat
    13861 gctgcgccga ggctcgaccg cgtcgcgcct tcctcacgcg cgttctcgac ggcacggcgg
    13921 gcgagttccg tgcctgagac tttcggccgg gtattcaccg ttgacgtgga ggccgtcgac
    13981 accgcgccgc cgcgtccccg acgtgcctcg gctaccagct caggccgtgg atcgggccgg
    14041 tggcacacgt cgtcgaagaa ggcctgtgcg atcgcaccca tcttcttcgc gtacctgcgg
    14101 tcgccgggtg cccactcccg ggccaggtcc gtgaatgtca ccggccgatt gagcttcctt
    14161 tggaagctgt cgagaatgcc ccattcctgt accttgcggg tccgatccgc tactgggtcg
    14221 gcgatgtagt cgcccgcata gagcagcaga tgctgcatgt gcgccttcac gcccgtcgaa
    14281 atgtcgggga aggactcacc ccgctggccg cgccccgcag cacccaggcc tgcgaaattg
    14341 ttctgtgacg gacgcacatt gcccggctgc ccattgccgc gcgtgaaaga cagattgccg
    14401 gtctcgagca gcatctggaa gaaagcatag tcccagcgga cgccaagctc ctctccgtgc
    14461 cgcatgtact cggtcgcgac tccatcgaag cggggatcca acgaggcatt acgatgtctg
    14521 acgaattcca tcagccggcc aggagtggca caggcggcga cctgattgcc gggccctgtc
    14581 ttgatggccg gcaaggattg cgccatggcg ggtaacgccg tccacgtcag cagcaacagt
    14641 gaggcagcgg ctcggcattt catgggacga tgaaactcca aacacgtcac attgcgaccc
    14701 gcatacgggc gcgcgctatc tccatagtca ttatggcgat caaccttaag gtccggttga
    14761 cgccagtgca cttgtgagac ttagtaggat cacgaacttt cgggcgcaag tttgtgcgcc
    14821 acggggtaga attgtggcct tgacggcaca gtccgataaa aaatcagtag tccgccttgc
    14881 ggcgctcgat ttcgattcct accgcagcgc aggatgggag gacgtcgggc ttttccacgc
    14941 gcacgcggat gctccgaacg cgggggtcgg agaggcaggc ttccgcgatc cgctcggcca
    15001 aggtttcgat gaggttgaag tggccgctgg cgagcgtctc cttcacggcc tggatcgcga
    15061 ggtcgtaatc gtagacctct gagatgtggt ccgacgcgcc atcgtagccg tcgcggattt
    15121 ccagatcgag cgagacgacg acccgttgct cgtagcggtg ctcgagctcg tgaacgccta
    15181 cgcttccgat cagctcgagc ccacgcacga agacgcgtcg agagcccgcg accgctggca
    15241 agcgttcggc ttcggcaaga gcttcgtcac tccaagggtc tacgctcata cggctcccaa
    15301 ccgctggatg cctctgactt tgcggcctga tctggccggt gccggaccag ggcgtcaagt
    15361 ccatccatgg acgcaaggcg gcctgcttcg ttccgaacga cggcgggttg cgtcgtgctg
    15421 ccgccggggt gcccgccttg cgaaggggag ttgcagcgct ttcggatttg cccgcgggag
    15481 cttccagcgg atccgcccaa gcggccggcg ttttccccaa gccgctcttt ccctccgctt
    15541 gcggtggacc tgcaacgaga ttaactctgg ccttggcaga acctcggggt atgtctcgtg
    15601 gtcctcacca tcgccattct tgtgaccctg accgtcgcga tcgcagcatt cgtgctcgca
    15661 ccgcgtacgg ctcggcgacg ggtcctcttc gatgcggccc cggatcggcc ggcccccttc
    15721 ggcctcaaga tggcgtggct cgccatccgg acgcaggaca cggcccgagt cctcgacgtg
    15781 ctcgggatgg tcgatcctcg gccgtgcaat tggaattccg gaatcggcac ggtctatgac
    15841 gaccgcctcg gcgagcacca cctgttcgtg tcgccgccgg tcgacggctg gacattcgtg
    15901 gtcggactgt cgctgcccta tcccgtcggg caggggttcg tcgacaaatg cacaccgatg
    15961 ctgctccgcc tcgggcgcga gttcggggag gtgcagtact tcttcagcta tccgctgatc
    16021 gacttcttcg cctgggcgcg gatcatcggc ggcaagctgg tgcgcgcctt cgcgatcagc
    16081 gatgagggga cgatctggaa caagggaaag ccgacgaaag aggagaggag tcttgggctg
    16141 aagctgttcg agctgcgcgg ggtgcgcgag cgcacagggg atgccggcgg agcactgatg
    16201 ctctatccga cggaggagca cgtcatgcgg cttgccagcc gctggagcct cgatccaacc
    16261 cgaatcgact cagcgccggc ctcggcatcg accggctata tctgtctcgc ccccacatcc
    16321 tggcggcctg agcggatgcg gaagtctgcg tgagggtcct cgcgccccgt cctcaatacc
    16381 agccgatttc aggcggccac accgacgatg tcagccattt gctttgcgat cagcgtctcg
    16441 agcgagccct cgaacgcaac cgtgaacacg ctgagcccgc gtggatcgtt tcgccaggtc
    16501 tcgaaagcgg cagcaaagcc ggccgggaga tccgcctcgc catccggcgc gacgacgctc
    16561 gcagccagcg ccgaggcaga cagcggcagg aatggaatgg ttgccgtctc gatgcttcca
    16621 tccgaacggc gggccggtgc tccgaccacg gcgcaacaga tgaccggctc ccggtcggta
    16681 aaggttacga ctgcgccgac gatgatccgg gagtgctcga accctgccgg tgcccggtag
    16741 gtccagcgct cgcctgcgcg aaagatcgga ggtgtcgtca tgcgccccgc cccagcctct
    16801 tcgagaggaa aaccgtgccg gcaagatccg ccaggatcaa ccctcggcgg cagccgttct
    16861 ggggaaaagg agctgaaaca tcagaaatct ggcagaggcg ccacgccgcg cgtcattcca
    16921 gtcggtcctg gcggccgctg gtcccggcgt attcgagctc cggagcatcg gccaggcaat
    16981 cagccggtcg ggtgagctgg tccgcccggt ccagaacgag cgcggtgcag tttgtcttca
    17041 ccagcctgcc actgtcggag cgcgtcacag caggaagcct gtcggccagc tttcgcggag
    17101 ctgtcgtgat cgatcccgtg tattccggcg ccgctccgcg cgcgagcaca cccagcccgg
    17161 gtccataccc cgccaagctc caaaagaccg tcgcaagcgc gaaaccgacg acgctccagg
    17221 tgatcgccgc gcggcgcgtg ttggccggac cggctgggtc gcgatagggt tgcctcagga
    17281 ttcccatgga gttttgtccc gccaagcgaa tctgttgcgt tctggcgcca tgatgctgga
    17341 tcgccaccac accgcaatgg cactgaggtt acagtcgccc ggcctgacca tttctccacc
    17401 gaaatctcgg cgtgtgttgc tgtcaaagtg cacgtgagcc tggaagcatc gaccggccgc
    17461 tgccggcacg aacgggtggc acgtacatgc ggttggcccg gcaggagctg cggcgctcgt
    17521 tgctaccgca ccggcggaat tcgccttcgc gcgaaaagca gatgcgcacc tcccgcaggc
    17581 ggttgcccga gccgcggcag tcgacggcga gcatctccag cttcagacca ggattggcct
    17641 cgaggaagct gtctgcaacc tcctggggac tgacgaaaaa tggcgcggcc ggggagcgga
    17701 agcgccgggg aatgctgatc ttctcataga gccgccgcga aaggtcgtaa tagccggccg
    17761 ggtcgagccc ggtgcaggtg ccatgcttcc ggtactcgtg aatgacgagc cgcgggctcg
    17821 gcatgatgtc cagcatccgg tcgatgatcc gctgtggaac gaacggccgc tctttcgtcg
    17881 ggcagtattc gggatagccg cgctcgtact gcggccagag gccatgcaac acgaacgcgt
    17941 aagggcgcgc ggtgcgccgg cactgcgggt cgttgtcgtt cgcggcgagg ctcgcgcagt
    18001 aggtcgggct ccaggacagc accagcgtgt agtagtcgaa cgccccggcg cggtgctccg
    18061 gcgcgcggct gcgctgcgca aagccgggtg aagtcatgaa gagggcaagg ctcagtccaa
    18121 ggaggatcga cggccggagg gaacgtattg cggctttcat ggtcctagct cagcgaacgt
    18181 tttgcgatgg gctcaggtgc tagcgaggcg ctggaggatg cgggcccagc ttcgggcacc
    18241 tttgcggaag ctcgtcagat tgtatttctc gttgggcgaa tggatgcagt cgtcgtcggt
    18301 gccgaaaccg atgagaaggc tatccatgcc gagctgattt ttgaacgtgg tgacgatcgg
    18361 aatcgaagcg cccgagccca tgagggcggt cggccgctgc cactcctcgg cgagtgcctc
    18421 ggcggccgcc tggaaattcg gcgcattcgg atcgaaggcg attgcggcgt tgccgtggac
    18481 ggaaccgaac tcgaccttgc agtcgggtgg gattcgctgc gcaagataac tctgtaggct
    18541 ggcgatgatc ttctcggggt cctgcccggc cacgagccgg caggtgatct tggccgaggc
    18601 gcgcgaggga atgatggtct tggtgcccgg gccctgatag ccaccggtga tcccgttgaa
    18661 ctcgagcgtc ggccgcgccc agagctgctc cagcgctgtg aagccctttt cgcctgcggg
    18721 gacgctgagg ccgacctcgc caagaaagga cttttcgtcg aacccgagcc ggctccacag
    18781 ctcggcctgg ctcttcggga cgtctgccac gccgtcgtag aacccgggca ccgttacacg
    18841 gccatcatcg tcgtgcaggg cagccaacac gtgcaccaag acccggatcg gattggcagc
    18901 ggggccaccg tagagccctg aatgcagatc cctgctggcc ccggcgacga cgacctcggt
    18961 gccggcgagg ccccgcaaca tcgtcgtgat gcccggggta tccttgtccc attgggtggt
    19021 gtcgcagacc agcgcgaaat cgcaggtcac ctcctgggca tgcttctcga gaaaaccggg
    19081 caggctcgga gatccgcact cctcctcgcc ttcgagcagc accgtgacgg cgaccggaag
    19141 cgcacccgcg acctgcttcc aggcgcgcgc ggcctcgagg aaggtcatga gctgaccctt
    19201 gttgtcctcc gcgccgcggg cgacgatcac tgtcccgttg cgcgaatcct cggccaaccg
    19261 cggctcgaac ggcggagcgg tccacagctc caggggatcc gggggctgca cgtcgtagtg
    19321 cccgtagaag agcacgtgcg gcattccctc cgggatctcc ttccggtcat gccccacgac
    19381 gatcgggtga cccgtcgtcg cccgcaccga cgcctcgaag ccgatctcgc gcagctgggc
    19441 ggcacaccac tcggcagcgg ccctgcactg atctttgtaa gcggggtcgg tcgagatgct
    19501 cggtatgcgc aggaacgcaa acaggcgctc gagagcggcg tcctgtgatc tgtcgagtgc
    19561 gtcgagaacg gcggagagct ggtcggccat cttcctgtgg gctcgcttcg gtcgtgctgt
    19621 cgtggcccca agagttgatc ggctgcggcg ctctgtcaaa ggaaagcgaa ctcgagcgag
    19681 ctcttgcgcg tttgctcccg gccgcccggg cggctattca gtggcagccg agtgcgtgag
    19741 ctgctgcaga cgaaagggcc accgaatgag cgactctcgt tccgaatccg ccaagcagca
    19801 caagtgggtc tacctgttcg gagccggccg ttcggacggt tctgcggaga tgcgaaatct
    19861 cctcggcggc aagggcgcca acctggcgga gatggcaagc ctcggtctgc cggtgccgcc
    19921 gggcttcacg atctcgaccg atgtctgcac ttattattac cagaacggtc gcacgttgcc
    19981 ggagggcctc gatgctcagg tcgagagcgc gctgggcatc ataggggatg cggtcggcgc
    20041 cgtgttcggc gatgcgcgca atccgctgct cgtgtctgtg cgctccggcg cccggacgtc
    20101 gatgcccggg atgatggaca cgatcctcaa tctcggcctc aacgatcaga cggtcgaggg
    20161 gctcgcggcg cgctcaggcg atgcgcgctt cgcctatgac agctaccggc gcttcatcca
    20221 gatgtacagc gacgtggtcc tgggcgttga ccacggcgtg ttcgaggaca ttctcgagag
    20281 ccacaagaac ctgaatggtt tcagcaccga tacggagctg gccgcggaag actggcagga
    20341 ggtcatcgcc gcctataagg aggcggtgga gcgcgagacg ggccagccct tcccgcagga
    20401 cctgcacacc cagctctggg gagccatcgg agcggtgttc ggctcctggc agaatgcccg
    20461 ggccatcacg tatcggcgcc tgcaccagat ccccgactcg tggggcaccg cggttacggt
    20521 gcaggccatg gtgtttggca acatgggcga aagctccgcg accggcgtcg ccttcacccg
    20581 caacccctcg accggcgcca aggagatcta cggcgaattc ctggtcaacg ctcagggcga
    20641 ggacgtcgtc gctggcatcc gcacaccgca gaacatcagc gagaaggcac gcatcgaggc
    20701 cggcgacagc gcgccttcgc tcgaggcact catgccggag acgtttgcgc agttgaagga
    20761 catcttcgcc cggctcgagg cgcgctaccg ggatatgcag gacgtcgagt tcaccatcca
    20821 gaacggcaag ctctggatgc tgcagacccg gtcgggcaag cgcaccgcga aggccgcgct
    20881 gaagatcgcc gtcgatctcg cccacgaggg cgtcatcagc cgcgaggagg cggtcatgcg
    20941 gatcgatgcc tccacgctcg atcagcttct ccatccgacc atcgatccat cggctcataa
    21001 gcacatcatc gcgcgggggc tggctgcttc accgggtgcg gcgtcgggcg agatcgtgtt
    21061 caactccgat gaggcggagg cattgaaggc gaagggccgc gaggtcatcc tcgtccgcgt
    21121 cgagacgagc ccggaagaca tccacggcat gcacgccgca gctggcatcc tgacggcccg
    21181 cggtggcatg acgagccacg ccgctgtcgt cgcccgcggc atgggccggc cttgcgtttg
    21241 cggcgccggc cagctccgga tcgacagcaa ggcgggccag atgctggcgc ccggtggcaa
    21301 ggtcttccgc gccggcgaca tcatcacgat cgacggttcc accggcgagg tgctcgaagg
    21361 caaggtgcgc atgcgccagc cggagctgtc gggcgatttc gccacgctca tggcctgggc
    21421 cgatgccgtg cgcaagctcg gcgtcagggc caatgccgac accccgcgcg acgctcagca
    21481 ggcgcgccag ttcggcgccg agggcatcgg cctctgccgc accgagcaca tgttcttcga
    21541 ggcggatcgc atccttgcca tgcgtgagat gatctgcgcc gaggacgaag agggccggcg
    21601 gcatgcactc gcgaagatcc ttcccatgca gcgggccgat ttcgaggcgc tgttcgaaat
    21661 catggccggt ctgccggtca ccatccgtct gctcgatccg ccgctgcacg agttccttcc
    21721 tcgcgaggag aaggagcaga cagccgtcgc cgaggcgctg ggcgtgccgg tttcccggat
    21781 caggacgcgc gtcacggagc tgcatgagtt caacccgatg ctgggcttcc gtggctgccg
    21841 cctcgccatc cgctatcccg aaatcaccga gatgcaggcg cgcgctattt tcgaagctgc
    21901 ggtgaatgcc gaaaagagga ccggcaaggc tgtgatcccc gagatcatga tcccgctcgt
    21961 cgcctaccgg aaggaattcg acatcctccg cgacgtgatc gttgcaacgg cggaggccgt
    22021 cgagaaggag accggggcca ggctgcagta tcagatcggc acgatggtcg agctgccgcg
    22081 gtcggcgctg cgggccgatg agatcgcgga gggccataat ggtgccgagt tcttctcgtt
    22141 cggcaccaat gacctgaccc agacctgtct cggcctttcc cgcgacgatg cagcgccaat
    22201 cctcgaaagc tatctcggtc gcggcatttt cgagaccgat ccattcgttt cgttggatcg
    22261 cgacggcgtc ggggagctgg tccgcatcgg cacggaacgt ggccgcaagg tgcggccgga
    22321 tctcaagatc ggcatttgcg gggagcacgg cggcgaccca cactctgtcg ctttctgcca
    22381 cgaggtgggg ctcgactacg tctcctgctc accgtttcgt gttcccgtag cgcggctcgc
    22441 ggcggcccag gcagccattg cagggcgcgt gacgccgaag gagggatgag cgcgggggat
    22501 tgcctcgaag gagttttttg aacgcgacgt aagactgctc ttcgccctat gcgagaggat
    22561 gccggcgctt gcgaaaaaac cctctgttgg ctaatgcatt gatttgtctc aggcgtggac
    22621 gggaaatcat gaatctcatc actcatgaaa agcaggaggt cgtcgactgg cggccaggcg
    22681 ttgcaacgcg catgctggtg tcggccgtga ccggctccgc gcagctttgc gttttcgagc
    22741 agtactgcga cccgggactc ggagcgccga cacatttgca cgccgtcgaa gaggtgctga
    22801 cggtggtcgc aggcaaggcg gagatatggg tcggcggcga caagattcag gctggtgccg
    22861 ggcattcggt catcattccg gctggtgtga aacacggctt ccggaatatt tctgagaacg
    22921 aaattttgca tatgcgcgcg acgctcgcat cgccgatttt cgaggcgagc tatgacgatc
    22981 acagggagca gtcccggcgt tacgttccgc aggactgagg atcgcaccgg gtttagcggc
    23041 attgtgcctg ccgactccgc ggagtccacg agcacagtcc tgaatagcgc atcgagagca
    23101 cgcagcctca cgagcacttt tctctttcga gcaatcgcaa tctcctaacc gtcagccgtg
    23161 cacgtcgcgg gcaggtatcg tgttcgcgga ttgccgagcg cgtgattcga gcgtcggcgt
    23221 tgacgggccg cgccgcgccc tgtactgaca tgcaggtaag tatgctggga gcgttcgagg
    23281 tctggaaatg agacttccgg ggaagattgc catcattacc ggggcggggt ctggcatcgg
    23341 ccatgagacg gcgaagctgt ttgcaagcga gggtgcgacg gtcgtcgtcg ccgatcgcaa
    23401 ccgggatgcg gcggaacgcg tggccgccga gatcgtgaac aatggcggca aggggtctgc
    23461 cgccagggtg gacgtcacgc gcgaagccga agtcaaatcg ttgatcgagg gcgtcgcggc
    23521 cgagcacggc cgcctcgata ttcttgtcaa caacgcgggc tacggatttg ccggcaccgt
    23581 cgtcaccacg accgaagccg agtgggacgc cttgatggcc gtcaacgtga agggcgtgtt
    23641 cttcggctgc aagtacgcca ttcccatcat ggagaagcag ggtgggggcg cgatcgtgaa
    23701 tacagcctcc acggtcgccg ccgtcggcat caccgaccgt gcggcctacg ttgcctccaa
    23761 gggtgcggtg gcggcattga cgcgtgcgat ggcgcttgat cacgtttcgg cgaatatccg
    23821 cgtcaactgc gtcgctccgg gtacgatcga gtcgccttac ttttcggaaa ttttcgcgaa
    23881 agcgccaaat cccgatgagc tgcgccgcag tctggagcag cgccaggcga tgaaccggct
    23941 gggcaagccc atcgagattg cgcgcgccat actgttcctg gccagtgacg aatcttcgtt
    24001 ctgcaccggt acgatgttgg tcgcagacgg tggctggacc gcgcgctgac gaggacgctg
    24061 aattaaatca caggatgatc tcggatgtat ggacttgaag gccgcaaggc gatcgttacg
    24121 ggtgccgccc aaggcatagg ccgggccatt gcggaacggc ttgtcgcgga aggctgcgac
    24181 gtcggcatat tcgaccggga tgccgaaggc gcgcgggcga cagccgatgc gctcaggact
    24241 ggaacgcgca ggatagcggt cgcgagcggt gatgtttcgg tcaaggctga tgtcgaaaga
    24301 ggcatggctt ttctgaaagc cgagcttgga cccatcgaca tactggtcaa caatgcgggg
    24361 atctgcccaa tcggcaagct tctcgaaatg ccggaggagc aatggcacgc aacgttcggc
    24421 gtcaacgtga acgggctgtt ctacatttcg cgcgccgtcg tcccggacat gatcgcaagg
    24481 cggcgcggcg ccgtcatcaa tcttgcttca tggatgggca agtccggcgt cgccagctac
    24541 ggagcctact gtgcttcgaa attcgcgatc gttgcgctga cgcagactct ggccgtggaa
    24601 atcggcgagc acggtgttcg ggtcaatgcc gtcgcgccgg gtttgattgt cgaaacgaga
    24661 atgcgcgaag agtcggaagt gcaccggcgc gccatgggat tgtccaccgc agaagatcgg
    24721 gcaaagacga tccctttgcg tcgcgccggg ttgccgagag acattgccaa tgcggtggcg
    24781 ttcctggctt cggatgaagc ggattacatt acgggcgaga cgatcagcgt gacgggcgga
    24841 agctggaacg actgattttt ttgtgggcgg agcgcgagca caccccgcga tttgagaatc
    24901 tcggcggacg acagctatgg cgaaaactct cccgcgcctt ttttcgcctg gcagcattgg
    24961 gccactcacg ctcaagaacc gcgtaatcat gccgtcgatg acgacgcgca gggcggacgc
    25021 ggaggggttc gtcacagagg ctgggatcgc ctattacaaa gcgcgggcca aaggcggcgt
    25081 cggtctcata accgttgaaa tggcctcacc cgagcgggcg ggaaagcacc gccatttcga
    25141 gcttggcatc tatgacgacc gctttctgcc cgggctgagg cggctcgtgg atgtcatcca
    25201 cgaagagggg gcaaaggcct cgatccagct tggccatggc ggcgggcaca cccggatcga
    25261 tatctcgggc gaaaagccga tcgcaccatc ggcagttccg cattcggtgc aggaggggca
    25321 taccgaggtc atcattcccg aagaaatgac catttcccgg atcgcacaaa caacagaggc
    25381 gtttgccgat gccgcgcgac gcgccgctgc cgccggcttc gatgccgtcg aaattcacgc
    25441 ggcacacggc tatctcattt cgcagttcct gtcgcccaag gaaaaccagc gtcaggacag
    25501 gtacggtggc agcctggaaa accgggcgcg ttttgccctc gaggtcacga ccgcggtgaa
    25561 aaaagccgtc cccgatctcg cggtcatttt ccggatgaac ggcgatgatt ttttcgaagg
    25621 gggcatgacg gcggacgagg ccgtggaggt ggccaaatgg gccgcagacg caggtgcgga
    25681 tgccatacac gtgaccggtg ggcactatcg ctcgcagccg accgcggcca tcatgatacc
    25741 gccgatgtcc acgccgccga cgccgttcct cggtttcgcg gaagccgtga agcgtcgggt
    25801 gcgcgttcct gtcatcgctg tcggtcgttt cggaaatccc tctacggcca tttcggtcgt
    25861 cgaggagggg cgggtcgatt tcattgctct tggccggccg ctgctcgccg atcctgactg
    25921 ggtcaacaag gctgccgagg gccgtcaggt ccggctttgc ctcgcctgca atacgtgcgt
    25981 ggatggcatg cgtaccggcc ggcagctcca ttgcctggtc aacagccgca ctggccgcga
    26041 gctggcctta tcggacaggc cgccgcgccg caacggtgag cgcattgcgg tgatcggcgc
    26101 gggaccagcg ggtctgacct atgccgcgct gatggctgaa gcgaacgagg tcacggtctt
    26161 cgagaaggaa gatgaggtcg gtggagcctt ccgctatgcc gggttggccc cgctgtttca
    26221 ggacgtcgag gcaaatcccg acagtctgct ggcgtatgta gagggcctgc gcacggagtg
    26281 cgaagagaaa ggcgtagagt tccggatggg ctgcgacgcg ctgatggacc cgaagcttct
    26341 cgagagtttc gaccatgttg tgatcgcaac cggggcgcgg tatcgctggg ggcttggagg
    26401 cgtgatcaaa cgcctgctgg tctcagggcg cgtccgccgc gagccgctgg cccggtttgc
    26461 acgaagtgac cgattcagaa attggttcta ttacaggctg cggtttggaa caggtcgcga
    26521 tgctatcgga cggttgtcgg gcagccgttc cgtcgaggcg atcggagatg cggtaaacgc
    26581 tgggaaaagc gaggctgcca tcatgagcgc ctatgctgcc gcttttggac cactcgacga
    26641 gaccgctgaa gaggcaagga cgcatagcgc aagcgggtga gaactgtgga gctcggtatg
    26701 aaagagcgga tcaaggagat cgcagtcgag ctcctggtcc gcgatggcta tcaagggttt
    26761 cgcttccgcg atcttgcaga tcggctgggc attacccgcg cgaatatcca ctactactac
    26821 ggcagcaagc tgaacctggc cgaagaggtc atcgtggact atgtccgcgc gacacttgcc
    26881 gcgtgggaag agaattggag gagcgacaaa accttcgagc agaagatccg tggcatgatg
    26941 gagtccaacc gccagcgtta tcttcgcttc aatcccaccg gcacgaccgg gcacccatgg
    27001 agcctgatcg gccgcatgcg catggagcgg gacgtcatcg gtccagaagc ccgcgcggcc
    27061 ttggtcgagt tcggcgtcgt tctcgaagac ctcgtggtca aggccatcga gaaagccatt
    27121 caggaaggcg agctttctcc cgagatcccg atccgcgata tcgcattaca actcgtcgcc
    27181 atcgcgaaca gtgcgggacc gataacccag gatggcggct cgttcgaccg cctggagcag
    27241 ctttatctgt ccttcgcccg catcgtggac catgcctacg gccgcaggcc tgggcgaaag
    27301 caaggccgct cggaggttgc caaagccgcc aagcgaaagc ggccgtcaca gccacgttca
    27361 ggcgctacgg tcgggacgtc tggcgaaatc gagcagaaac aatctgcgcg ctagaactcg
    27421 gccgcatctt cctcgtcgag ggtgcggcgg cgggggcgat ggggcagcgg aggtagcaac
    27481 aagagcacca cgatcgccgg cggaaagagg aacgaccacg ccatccagaa cgagtagtcg
    27541 cgcttcttca gaccggcaag caggccaccg atcaccatcg agccgaacgc aaccaggccc
    27601 cagtaaacga tgacctgtat catcccctcc tcctaccgcg ccgggtggtg cgggtgccgt
    27661 gtcaaggaac cgatatcgcg ggcctaagcc gtgcctgcca agtatggcga aacggcgggc
    27721 agcgctcgca gcgatgacgc tatgccatcg tgccgacctc gatgtccaat ccgatgtcca
    27781 gcaccgtcgc cgagtgggtg agggcgccga ccgagatgag gtcaaccccg gcctctgcta
    27841 tcgcgcggac cgtatcgaga ttgacgccgc ccgaggcctc cgtgatcgcc cgccccgcaa
    27901 cgatgcggac ggcctccgcg agctgatcgg gcgtcatgtt gtcgagcagc accgcatcga
    27961 tgcgatgctg aagcgcctct gaaagctgat cgagcgtgtc gacctcgacc tcgatcttga
    28021 ccatgtggcc gacgcgggcc cgtgctgcag cgaccgcggg accgacgcct cccgcaacgg
    28081 cgatatgatt gtccttgatg agaacggcat cgaagaggcc gatgcggtgg ttcattccgc
    28141 cgcctgcgcg cacggcgtat ttctcgaacg ccctgagccc gggtgttgtc ttccgtgtgt
    28201 cgacgatctg ggcacgcgtg ccggcgacgg cgtcgacata gcggcgggtg agcgtcgcga
    28261 tcccgctcaa gcggccgaga aagttgaggg cgacgcgctc ggctgtcagg agcgcgcggg
    28321 cattgccgct gacacgcgca atgatcttgc caggttcgac cgtcccgcca tccgggacga
    28381 gaacatcgaa cctcacctcg ggatcgagat gccggaaagt ggccgcggcg acctggagac
    28441 cggcgactat ccccggcttg cgcgccgcga acacgcccga tgcggtcgcc tccggtgcga
    28501 tgacggcgtc cgtcgtcacg tcaccggcga ggccgagatc ctccgccagc gcgccggcga
    28561 cggtgctggc aatcaatggt tcgggcaggc ggagatcgaa cgcgcttctg ggtatcatgt
    28621 gcatcaaatc ctgtctgtcg ttgcgccgat cacggaggcg agctcaccag ccttcagggc
    28681 cgacctgaga tggaggccag ccagtgtgat cgcgctgcgc cttagccatt gcgggtccgc
    28741 ctgcggatag tcgctgcggt agtggccgcc ccggctttcc tttctgcgca acgcgcactc
    28801 cgtgatgaag cgcgccgcga tcgtggcatt ctcgatcacg gggtcaccgt cggcggcctc
    28861 gccgacggac tccaggatgg ccagcgcctt ggtcagccct tcggcgttgc gctcgactcc
    28921 gacgtggcgg gacatcgccg tacggatgac gcgaatggcc tcctcgcggg ctgcaaagtc
    28981 gggcagatgc gtggttcgcg tcctggtaac agcgacgcgc gtggtcctgc cgcgctcgag
    29041 cctgccaatg tcagcggcga cccgtgcgcc gaagacgagc gcctcgagca gggagttgga
    29101 tgcaagccgg ttcgcgccat ggagtcctgt gcaggcgacc tctcccgcgg cccagaggcc
    29161 gggcacgctc gtgcggccca atgcgtcggt gcggaccccg cccatgtggt aatgctcggc
    29221 cggcgcaatc gggagcggct gggtgacggg atcgaggcct gcggcgcggc agtattcgaa
    29281 gatggtcggg aacgtctcgg gaaagcgggc gccgatggct tcgcggcagt cgaggaaggc
    29341 gccgcgaccc gcagcgattt cgcggaatac ggcccgtgcg acaacgtcgc ggggggcgag
    29401 ctctgcatcc gggtgctgat gaagcatgaa gcgctcgccc gcgccattga tcagcaccgc
    29461 gccttcaccg cgcagggctt cgctcgccag cggcgcagga tcgaggttca cgtcgagggc
    29521 cgtggggtgg aactggacga actccgcatc cgcaatggtt gcgccggccc gcgctgcgat
    29581 cgcgatgcct tcgccgcgtg catgcggtgg gttggtcgtg acggcgtaca gcccgccgat
    29641 tccgcccgtc gccagcacga cggcgcgcgc gccgtcgagc acatagatcg cagatgggtc
    29701 atcggcacgg gcgagattga cgcccgtaac ccggtctccg gtcttgttca gagacagggc
    29761 cgtgtaattc tccagaacgg tgatcgatgg agcctgcctt acccagtgga tcagggcttg
    29821 catgatggcg cgaccggcac gatcgccgga gacacgcacc acccggccgg ctgaatgagc
    29881 ggcttcgcgc gagagaacga agtgcccttc gagatcacga tcgaacggga cgccataggc
    29941 caagagatcg cgtatgcggt cccgagcttc gcacgcgacg atgtgagcca cgtgctcatc
    30001 gacgatgccg gcgccggcgc ggatggtgtc ggcagcgtgt gcctcaggcg tatcgccttc
    30061 accaacggct gccgcgatgc caccttgcgc ccaggcgctc gacgtgccct cgccgagggg
    30121 ggccggggag atgagcgtga ctggagccgg cgcgagcttg agggcggtga acaggccggc
    30181 gaggcccgcg ccgatgatga cgatatcgcc cccagcagct ggctgattta cttgtccgcc
    30241 cttgatcatc gaggtgtgct cgagccgccg cggctgaaag gggaacgcgc ggcggctctg
    30301 aaatcctcag ttggtgagat tgatcatgcg ctcgacggac cggcgcgcgc gctcggccac
    30361 gagcggatcg acggtcacct cgtgacgcat gtagagcagg ctctccagga tgttctgcag
    30421 cgtgatccgc ttcatgtgcg ggcaaagatt gcagggcctg acgaactccg tcgtgggtgc
    30481 ctcggcggcc acgttcgagc tcatcgagca ctcggtgacg agcaccacct tggccggctt
    30541 gtgggtcttc acccagttga tcatgcctga cgtcgagccg gcgaagtctg ccgccttgag
    30601 aacgtcgggc gggcactcgg gatgggcgat gatcttgagg ccaggatcag ccgcccggaa
    30661 tgcctcgagc tcctccgccg tgaaccgctc gtggacctcg cagtgcccct tccacgtgat
    30721 gatctcgaca tttgtttgcg tctggacgta cttggcgagg tattcgtccg ggatcatgat
    30781 gacccgcggc acgcccaggc tctcgatgac cttcacggcg ttcgccgagg tgcagcagat
    30841 gtcgctctcg gctttcactt cggccgatgt gttgacgtag gtcacgaccg gcaggccggg
    30901 ataggcctcg cgcagcatgc gcacatcctc ggcagtgatg gaggatgcga gcgagcagcc
    30961 cgccctgagg tccggaatga ggaccgtctt gctagggttg agcagcttcg aggtctcggc
    31021 catgaagtgc acacccgcct gcacgatcac ctgtgcatcg gtccgcgctg cctccttggc
    31081 aagctggagg ctgtcgccgc ggatgtctcc gacgcaatgg aagatctccg gcgtcatgta
    31141 gttgtgcgcc aggatcacgg cgttgcgctg cttcttgaga tcgttgatgg ccttgatgat
    31201 cggcgcgaac tgcggccact cgagcggcgt cacgaccttg gagacctttg cgtagagagg
    31261 ctccatctcg cgcgccagtt ccggcgtgaa cttcagatcg ggcattggca gggcgtcgcg
    31321 actcttggca gccggggcgc ggatttcggt ttgtaccgtg gccatttggg gatgctccct
    31381 cgcggtctcc acagaaatgc tcattttgag cattgtagtg gcatgaagaa ctccggataa
    31441 accggttgga tatgctcgcc ctgagcattg cttgtgcgga cttaagatat gacagttggc
    31501 ctccgattac tagaggtggg ttgatctgct cgcggagtgc agggatgcac ttttcgcgga
    31561 ccgccgccgt ccgttcactt atcctcgcga ccggccgtca ggagcagagg ctctgacagt
    31621 tgcgaccacc cgggctgctt ccatcctgtg gacacacggg aaattgcccg gaccaggctg
    31681 gatcgtggga cggagcttga tccagtggtt ccggcggctc tatcaaaccc gcccgggctt
    31741 cacgcggacg ccgggggcag ggcgttcgag caggacctcg cggcggaatc gatagagccg
    31801 cgccggccgc ccgccggtct tggcgcgcac ctcgcccgtc ggctcgacga gtccggcgct
    31861 ttcaacgagc cgccggaaat tctgcttgtg caggtgtggc ccgaggatcg cctccacggt
    31921 cttctgcagt tcgaaaagcg tgaactcgtc ttccatcagc tcgaatacga ccggccggta
    31981 tttgatcttg gcgcgtattc tgccgatggc cgttgcgagg atccggcggt gatcgaaacg
    32041 catgggagcg ccgaggcgcg gcaggttgcc ccaggtgagc gctgcctcgc ggccatcgcg
    32101 gcgggcctcc tcgactagtc cggcgtcgta aagcagctcg aagcgctcga gcaccttctc
    32161 ctcgtcccaa gtcccgccgt agaggccaaa gcacatgcgg agccgatcgg tccgctggat
    32221 cggtcggccg gacgaggaaa gaggaggccg atccgcccag gccatgagct tcggctcgat
    32281 cacctctgtc aggatcgccg gcttgccgcc ccgccaatcc tcccacggga agtaatcgta
    32341 ccaactgcgc cactcccagc gatccgagtc accgccgggg ccgggccgcg tcagcgcgag
    32401 ataaccaatc gagacgacgt gcgggcccgt atcgccgggc tgcgcgtgcc ggccacgatc
    32461 gccgaaggta tagagctgct cgacgtaacc gagctcgagt ccggtctgtt cggccaccca
    32521 cgagcgcaat ccgatttcaa gggtgcggtg ctcaagggga gaaaaaggcc caaacggaag
    32581 cgattcagcc tgatcgcctt cgtgcgagcg gaccaccagg accaccggct cgtccattcg
    32641 cacggctaga atagctgcgt tgaggccgat ctcgaccgct gccacctcgt gccgctcccg
    32701 tggttcgctc gcgcgcgctg acggccgtgt cgcctgcttc aggtgagctt cgaaggtcat
    32761 cgccgatctc cttcgcgccg cagtactgag ggatcgtggc gaatttgggt atcgcgcttt
    32821 tcagaggcct tgggctgttg ccaattcctc acggatcgaa atgcaagggc agcataaaag
    32881 ggcggccctc ggttgccggt tccggcggcc ccatttcttt tatcgcgtga agcatgcgac
    32941 cgcgccgacc gagcatgtca tcggccaggc gaatgagcaa attattgggg caggctgtcg
    33001 gcgaagcgcg cctcaatcgc tgcgccacca gccactccgg cgtttcagga tttatggcgc
    33061 aaagcgtgat aaacgccgca gccgtggaac ggctaatccc cgcccagcaa tggacgacca
    33121 gcggtccttg cctattccag tctctgacat agcggagcag cgcctcgacg tgcgcctcac
    33181 atggatgcac caggccctct tgcggcgcca cgatgtcgtt cacggcaatg cgcagatgat
    33241 ttgcagcgtc gatgccagct ggcgtttcca gcattgtgtg ctggttgatg agcgtaatca
    33301 gatggcgtgc gcccgtttcg gcgacgacat tcggcacgaa actgagcggg caaacatgca
    33361 acccgaccgg acccgaaccg gaaggaattt tctgagcagc catttgctga agctaaatcg
    33421 caccgaaatg cggcgtaatg cgctgcgccg aaccgatgct tttctcatga gccacacgac
    33481 tgatgcttct catgagccac attggcgggc gctggcaagc cactgcggca aaataacgga
    33541 attgtgatgc cggcgctcgc cgctatggtt tgggattgcg gtgtggtgag ccgagattga
    33601 cgccgctgtc gactggcgat ccgctcgtat cggcaggcgc tccgccggtt ccaattcccg
    33661 ggcggttgtt cggatcgaat ggcgtgtccg gtgagctggg ctgcggagag acggacgctt
    33721 ccaggtagct gtcggtgcct ggctctatcg ggttctccct ttcgtgccgc gtcggcggtg
    33781 gcggcgcatc ccggcgccgg cgcgcgcgca cgaccagggc gttctccacg cgggtgacgc
    33841 cctccgcctc gaaggcaatg acctcggcca tctgcttcgt gagctcgctc ggcacgctgc
    33901 cgcgcaggat gacatgtgcc ccctggacat cgacctcgat atccgacgcg tcgatgccgg
    33961 ggtcgtccgc aagtctgctc tgcacgtcat ctctgatgtg atctgcggca gggcggccac
    34021 tgctggtcga tccttccccg ctcaaccttc ttgtctccca gcccatcgtc tttctgctcc
    34081 atcccatttc ccgcgcaggg gcagctaatc aacgccggcc ctgcaggtcg gttgcatgtg
    34141 gccattttcc ggaaccctga caagcaggtg gcgtttcgaa agcgagtgcg tttgaggcca
    34201 aaaacccaag gactttcgat gataatttcc gaggtcatga cccgagaggt cgaggtcata
    34261 tcgcccgatg cgagcttgcg cgaggcggcg cggaagatgg acgagctcaa tgtgggtgtc
    34321 ctgccggtgt gcgacgggca gaggctggtc ggtatgatca ccgaccggga catcactgta
    34381 cgcgccacct cgattggcga atctccggat gtgacccggg ttgatgaggt catgtccgag
    34441 gaggtctggt ggtgctacga ggacgaggat gtcgaggacg tcgcgcggcg catgggcgag
    34501 aagcagatcc gccgcatgcc tgtaatcgac catgatcaca tgctggtcgg aatcgtcgcg
    34561 ctcggcgact tggcgaccga ccgcgccccc ggcacgcaga gggcgctgcg caagatctcc
    34621 gagccttcgg agccggacct gtcggggacc ctatcggacc ggcgggccga tccggcccac
    34681 tttgccctcc tggaagacgt ggctgaggag cgccccagcc gcaccgatga tgtcattgcc
    34741 gaggacgttt tcaggggact ggatgcggca gaggagatcg acgttgccga gatcgacatc
    34801 tacgtcgatc acggtgtcgt atcgctcaac ggaacggtcg acaattacgc tgcccaggag
    34861 ctggccacca agattgccga gggcgtcgag ggcgtcacca gggtcgacaa caaccttcgg
    34921 atccgcaccg atgccggcgt gctcccggac gtcaggttca agggcctgcg gacagccaag
    34981 taattgttgc ttcttcagct tacctgcggc gcccagcgcc gcaaagcgaa gtggaccgga
    35041 gcgaagatca gcaacggata gcctgccatc agcatcagca gccacatcca ctgcggcatg
    35101 atcgtctgag gctcggtgtc gctgaatccg tagacgtagt tgatgttgcg cgccgtaccg
    35161 ccagggtcgg ggctcggcgg gggcagcaga aagtacgaga tcagaaggac gccccaggcc
    35221 aggacggtcc acgcccagaa agcgcgggcc tgatagccga gccgccacac caggaacaac
    35281 agcagggccg gcagccagat gtggaatagc gacaacgcgc gcagataaaa cggatagccg
    35341 gaatcgaaca tgtagccggt cattcccgta atcctgcccc cgactgcaac ggcgacgaaa
    35401 tcggcgatcc acaaggcctg cgtcgcgagc gttccgaccg cgggcatgga ggccagcagc
    35461 gggctttcca gccagatcgc ggcaagcacg aaaaacagcg ccagatcact gaagaagaga
    35521 aaattggccg gtccgtagtg gtgccaatag atcggcacca gcagggccat gaatgcggta
    35581 taggcgagct tcagccagag tgggatagct cggccgcgtc ctccatctag gggtggcggg
    35641 tcgcgcatgc ggtcgagcgg cgctattgct gccgtcgcgt catgaatgcc agacgctcga
    35701 acaaatgcac gtcctgctcg ttcttgagca gcgcgccgtg gagaggtggg atcagcttgc
    35761 gggggtcatt ctcccgcaat gtctcgggac tgatgtcctc gttgagcagc agcttgatcc
    35821 agtcgagaag ctccgacgtc gaaggcttct tcttcaggcc cggcacctct cgaatgtcat
    35881 agaagatctt cagcgcctcg gaaacgaggc gtcccttgag ccccgggaag tggacttcga
    35941 cgatggaacg catcgtctcg ggatcaggga acttgatgta atggaagaag cagcggcgaa
    36001 ggaaggcgtc ggggagctcc ttctcgttgt tggacgtgat gatgacgatg gggcggttgc
    36061 gcgcgcgtat cgtttcccct gtctcgtaga cgaagaattc catccgatcg agctcctgca
    36121 gcaggtcgtt cggaaactcg atgtcggcct tatctatttc gtcgatgagc aggacggggc
    36181 ggacctccat ctcgaaggct tcccaaagct tgccgcgccg gatgtagttg cggatgtcct
    36241 tcacgcgctc gtcgcccagc tggctgtccc gcagacggct gacggcgtcg tactcgtaaa
    36301 ggccctgctg tgccttggtg gtcgacttga tgtgccactc gatgatgggg acaccaaggg
    36361 acttcgccac ctcgatggcc agcatggtct tgcccgtgcc aggctcgccc ttgaccagca
    36421 atggtcgctg caacgtgatg gccgcgttca ccgcgacggt gagatccttg gtggcgacgt
    36481 aactgtccgt cccctcaaat cgcatcagca actccataca tcatccgtcc ggcccagagg
    36541 tcgggcgagc ttcgaaagcg agcccctggc gagcggggac attagagatg aggccccggt
    36601 ccagcaaggc gggggtatca ggcgcgtgga cacaaaactg cgccacctcc tggcggggag
    36661 gtggcgcagt cggggtgctg gaggcacccg cggacggctg tcgtgccgcg cggcgcggct
    36721 gcagaccccg cctgcagcga tatggggtcg cgcacgcccg gcgatccgcg ccgtcctgtt
    36781 gcccgccggc cacgtgggcc ggcggggcgc cctacgcggg tggcgaggcc cgggtggacg
    36841 ctgcgccagt ctctccaacg gccctgaaga tggcttgaaa ccggaggcga aaactcgagt
    36901 aaaatgcgtg caaactcagc cgagtccggt cttcgtgctg caccccgttc gccaactgct
    36961 gacgacagca agggaaattc ttgacttggc tagttgtcag gctaaaagcg ggacaaactt
    37021 gtcatgctca gtatgcgcta gttgcgtcac tgcgccagaa cgtccgcagg ccaaacatga
    37081 gcactacaca agatcggaaa tcggtcgcga agaccaaagg caaatcggtg aaggtggagg
    37141 aagcaacgct gtccagcctg ttgctgccgg acgactatcg cccgtcggaa gacgagcctt
    37201 tcatgaacga gaggcagcgc gcctatttca ggcgtaagct gttggcgtgg aaggaggaaa
    37261 tcatactcca gacccgtgag acactgatgg gtctgcacga ggactcgacg cagcatgccg
    37321 accttgccga ccgggcgacg tcggagacgg atcgggcgct cgagctgagg gcgcgcgatc
    37381 gccaccgcaa gctcatcgcc aagatcgatg ccgccctcgc ccggatcgag gatggcactt
    37441 acgggtactg cgaggagacc ggggagccga tcggtctgcg ccggctcgat gcgaggccga
    37501 tcgccacgct gagcctcgag gcccaggagc gccacgagcg tcgcgagcgg gtctatcgcg
    37561 aggattgata tttccagcta cgaggcgggg ctggtatctc ccgcctcaac ctcctgctcc
    37621 ctgcggcgcg cctcgagggc gcgtgcgaag gcctgcttca cgccgtccag gtcgcgcagg
    37681 tggatatcgt gttgccgata ggggatctcg atcccggcgt cccggaacgc cttggcgatg
    37741 gccagccgga gctccgactg aacggtgagt ctgcgcatga cgtcgggcac gaatgcgcgc
    37801 accgagaaca tcatggcgct ttctccaaaa tcctcgaaca cgatcaacgg cgcgggctct
    37861 cggagcaggg atggcgagcg atcgaccaca tcggcgagga tcttcatgac ctgctccgga
    37921 tcggccttgt acgagacgcg gacggacacg atgaggcggc cgatcaggtt gcggtgcgtc
    37981 cagttcatca ccgtgccggt gatcaattcg gagttcggga tgatcagact ggagcggtcg
    38041 aatgtctcga tctcggtcgc ccgcacgttg atgcgccgga cgaagccctc ctggccgttc
    38101 acgacgatcc agtcgccgac cttgatgggg cgctcgatca gcaggatgat gccggagacg
    38161 aagttgttga agatcgcctg caggccgaag ccgatgccga gcgagagggc gccggcgacg
    38221 atggccagct tcgtgaagtc gagcccggcg tacgagaggg cgatcaccag agccaggccg
    38281 acgccggtgt agccgacgcc catcagcacc gagtgcgcga tcgacctgtc cacgcgggcc
    38341 gggccgagga ccgaccggtc gagccagcgc tggacgacgc gcgtcgcgaa cagcagcacg
    38401 aagaacaggg cgaacgaaag caggatgcgc cccagcgaga tctggaattg gccgatctcg
    38461 atcccgacga tgccgagcct cagccaatcc aggaagtcct cgcgcgtgta gccccaagtc
    38521 agcagaagga gcggcagggc agcgagcagc aggaggagct cgacgaggag gacgagcagg
    38581 cgcgtcagat agctggtgcg atcgtgatcg agaccgaggc gcgtctcgag gattcggccc
    38641 atcgggcgct cgccgtcggt catgacgacc gcgacgcgcc ggatcaccag atggacggac
    38701 aggatgaaga gccccaggcc gccgatcagc atgacctgcg tcgtaatgaa ttttccgagg
    38761 gcgacgtatc cggccagcgt ggtcgcgaca atggtaagtg ctgcgagcgc gatcggtatc
    38821 ttcagccagc gcgggcgcga ccgggacggc gcctcgagga gtgccgcctc gcggccggcg
    38881 aggggaaccc gcgtgaacgc gatcagcagg gtggcgaaag caaggctcgt cgccgtggcc
    38941 tgcaccgccc cgatctcgat cggcaggtaa agcatgcgca cgacgtcgcc gaggagcagg
    39001 tcgagagcga tcaggcccgc aagaaggttc gccaggaaca gcagcttggg cgcgctcgca
    39061 tcggcaatgg cgatcagcgg ctgtccggaa gccgtcgggg agaaggcggc cccggccagc
    39121 gcccgaatga tctgaaagac cacgaaggcg cgcagcgccg agagcgcgag ctcgcccgcg
    39181 gtcacggtga gcagcccgag ccaggcgagc ccgacgtagc cgacggtcgc tgctgccagc
    39241 ggcggaaacg caaggccggc ggcgagccag aatgcgtcga tcgcccgcgg gaaatactgc
    39301 ctcaaggcga tgggactctc atgaattctg cgccggatat gggaaagcgg caacttgaac
    39361 aaggcatagg cgccgacggc agcagccaac accagcccga ggccgacaag gttcgatccc
    39421 gcgagccgcc accactcggc cgccagtctc gcgatctgcc gccccgtgcg cggcagctct
    39481 gccgcgaacg ccttccaggt cgctggcgag agggggcttc ccgaatggcg tacgaggtca
    39541 cgggcgaaca gggcttggcg tagccgctgc acgtggccga cgagctggcg cgaacggacc
    39601 aaagccagct cgactgtctt gacggcacct tcaagctcgg cctggacggc tgtgagccgg
    39661 ctgcgctcgc tggcaattgc cggcgcctcc ggggccgcat ccttgtcagg gggtgggccg
    39721 agcttctgga cctgggcctt cacggccgcc aggcggggcc gcagggcgtc gatcacctgc
    39781 tgggcttcga cttcgatccg ctccagctcg aggcgctggg ccgtgagccc gtcctcgcga
    39841 gacttgaccc gctcgatgcc cttcgcggcc gactcgatgt cggatgcgac ccgcgccacc
    39901 ctctgggcga gctcggcagg aaagaatgac ggagccgcta tcgctgccgg ttgggcggac
    39961 gttggggcgg cagcctcccc cgttcccacg gtctcctggg caagcgttgc ggtgagcaga
    40021 ccgaaacagc ctgccaggac gatgatcagc cgggtcagcc caagcaccat ttctcagttc
    40081 cgatcaacgg ccatccccga ttgctgcttg agccgatcgc cggccgctcc agaccaccat
    40141 gcagacgtcg atgctcacgc cttgcccacg gcctcctgga gggaccgcgc cggccgttgg
    40201 aacatcggct ctgctggctc ggccatcggg ctctggctgc tgggagcttc cggcggctcg
    40261 atcccggtct cgatgaccac atccaccggc ccgcctgtca tgatcagatg ctctattccg
    40321 tcccgccgga tcagcagcag cttgcgccgc ccgtccacgt tcgcctggtc gaccacctcg
    40381 agacgcttcg gcggacgcgg accgaagaag ttgccggaga gcgcatcctt cagtgcgctg
    40441 ctcggcgaac cggtcgtgaa atacccgcgc aacagcaaac cgctcaccac caggagggcg
    40501 gcgatgaaaa tcacgaaaag cagccagtac aggatttcca tttggctcga cgcgggcgcc
    40561 cccgcgctcc tctcgatctg ctggttggag gcgtgcacgt tctcatggcg ccgcgagggc
//
LOCUS       DASVDU010000333         3558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1147398, whole genome shotgun sequence.
ACCESSION   DASVDU010000333 DASVDU010000000
VERSION     DASVDU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147398"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF226_22185"
     CDS             complement(<1..252)
                     /locus_tag="VF226_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7076758.1"
                     /translation="MAERVIKLPDIGEGVAEVEIAELPVSVGEMVREGDVLASVLTDK
                     ATVEIPSPTDGRVVWIGFKVGDKAAVGSRLMTLEVAEAAG"
     gene            complement(256..1269)
                     /locus_tag="VF226_22190"
     CDS             complement(256..1269)
                     /locus_tag="VF226_22190"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7076759.1"
                     /translation="MPTMTMIEAIRDAHDVMMARDENVVVFGEDVGYFGGVFRCTKGL
                     QEKYGRTRCFDTPINESGIVGTAIGMAVYGLRPCVEIQFADYMYPAYDQIVSEAARLR
                     YRSAGDFSAPITIRMPTGGGIFGGQTHSQSPEALFTHVCGLKTVVPSNPYDAKGLLIA
                     SIEDDDPVIFLEPKRLYNGPFDGHHDRPVVPWSKHPLSEVPEGYYTVPLGTAVVRRPG
                     SAVTVLAYGTMVYVAEAAAEEHGIDAEVIDLRTLLPLDTETIIASVQKTGRCVIVHEA
                     TRTSGFGAELAAIVQESCFYHLEAPIERVTGWDTPYPHAQEWDYFPGPRRVGEALKRV
                     LEA"
     gene            complement(1273..2505)
                     /locus_tag="VF226_22195"
     CDS             complement(1273..2505)
                     /locus_tag="VF226_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEX7076760.1"
                     /translation="MAVRAPLRLHVPEPEFRPGSKPDFSGVPIPKAGSVRRPPVDVPP
                     EEIRDLAYSIIRVLNRAGEAVGPWAGTLSDDELLAGLKTMMKVRVYDQRMLMAQRQGK
                     TSFYMQCTGEEAIACAFQTVLQKGDMNFPTYRQQGLLITAGYPLVDMMCQIYSNSRDP
                     LKGRQLPVLYSSKAHGFFSLSGNLGTQYPQAVGWAMASAIKGDTRIAAAWIGDGSTAE
                     SDFHAALVFASTYKAPVVLNIVNNQWAISTFQGIARGGSGTFAARGLGFGIPALRVDG
                     NDYPAVHAVAQWAVERARRNLGPTLIEYVTYRAGAHSTSDDPSAYRPKEESEAWPLGD
                     PIVRLKNHLIVKGVWSEERHNQAAAEVEAEVVAAQREAESYGTLHTGPKALARDMFMD
                     VFKQMPPHLRRQRQQAGF"
     gene            2698..3189
                     /locus_tag="VF226_22200"
     CDS             2698..3189
                     /locus_tag="VF226_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX7076761.1"
                     /translation="MTKLAPTRATMKRLDEIDRAILRELQKNGRLSNAELAERVGLSS
                     SPCWQRVRRLEESGYIEGYAAVLSQKQLGMPETVILEVSLERHDDEILEKFGRALADF
                     PEVLEAYLTTGEYDYFVKIAIDGTEGYERFLRERLYRIPGIRQTRSSFALRCLKRQIS
                     VTP"
     gene            3272..>3558
                     /locus_tag="VF226_22205"
     CDS             3272..>3558
                     /locus_tag="VF226_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7076762.1"
                     /translation="MSETPVRKIIHIDMDAFYASVEQRDDPTLRGRPVAVGHAGKRGV
                     VAAASYEARKFGVRSAMPSTTALRKCPELVFVPPRFEVYRAVSEQIRRIFA"
BASE COUNT          638 a         1143 c         1131 g          646 t
ORIGIN      
        1 accggccgct tcggcaacct ccagcgtcat cagccgtgat ccgacagccg ccttgtcgcc
       61 gaccttgaag ccgatccaga ccacgcgccc gtccgtcgga gagggaatct cgacggttgc
      121 cttgtcggtc agcacggacg ccaatacgtc accctctcgg accatctctc ccactgaaac
      181 cggcagctcg gcgatctcga cctcggcaac cccttccccg atgtccggga gcttgatgac
      241 gcgttctgcc atggattacg cctccagcac gcgtttcagg gcctcaccga cgcgccgcgg
      301 tcctgggaag tagtcccact cctgagcatg cggatacggc gtgtcccagc ccgtgacgcg
      361 ctcgatcggc gcctcgagat ggtagaagca gctctcctgc acgatggcgg ccagctccgc
      421 accgaagccg ctggtccggg tcgcctcgtg gacgatgacg cagcggcccg tcttctgcac
      481 cgatgcgatg atggtctcgg tgtcgagcgg cagcagcgtt ctgagatcga tgacctcggc
      541 atcgatcccg tgctcctcgg cggcggcctc ggccacgtac accatcgtac cgtaggcgag
      601 cacggtgaca gcgctacccg gccgccggac caccgcagtg ccgagcggca ccgtgtaata
      661 gccctctggc acctcgctca gcggatgctt cgaccacggt acgacgggac ggtcgtggtg
      721 gccgtcgaac ggcccgttgt agagccgctt cggctcgagg aagatcaccg gatcgtcgtc
      781 ctcgatggag gcgatcagca gacccttggc gtcataggga ttggacggca ccaccgtctt
      841 cagaccgcag acgtgcgtaa acagcgcctc cgggctttgt gaatgtgtct ggccgccgaa
      901 gatgccgccg ccggtcggca tgcggatcgt gatcggcgcc gagaagtctc cagccgaacg
      961 atagcgtagc cgcgccgcct ccgagacgat ctggtcgtag gccggataca tgtagtcggc
     1021 gaactggatc tccacgcagg ggcgcaggcc gtagacggcc atgccgatcg cggttccgac
     1081 gatgccgctt tcattgatag gggtatcgaa gcagcgcgtt cggccgtact tctcctgcag
     1141 gcccttcgtg cagcggaaga ccccgccgaa gtagccaacg tcctcgccga agaccacgac
     1201 gttctcatca cgcgccatca tcacgtcgtg cgcatcgcgg atcgcctcga tcatggtcat
     1261 cgtcggcatg gcctagaaac ctgcctgttg ccgctggcgc ctcaggtgcg gcggcatctg
     1321 cttgaagacg tccatgaaca tgtctcgcgc cagcgccttc gggccggtgt gcagcgtgcc
     1381 gtagctttcg gcctcacgct gagcggcaac gacttccgcc tcgacctccg cggcggcttg
     1441 attgtgccgc tcctccgacc acacgccttt gacgatgagg tggttcttca ggcgcacgat
     1501 cgggtcgccc agcggccacg cctccgactc ctccttgggc cggtacgcgg atggatcatc
     1561 cgacgtggaa tgggctcctg cgcggtaggt gacgtactcg atgagcgtcg gccctagatt
     1621 tctccgcgcc cgctcgaccg cccattgggc gaccgcatgc acggccgggt agtcgttgcc
     1681 atcgacgcgg agcgccggaa tgccgaaccc gagcccgcga gccgcaaacg tgccggaccc
     1741 gccgcgggca atcccctgaa aagtcgagat cgcccactga ttgttgacaa tgttgagcac
     1801 gaccggcgcc ttgtaggtcg acgcgaaaac cagcgccgcg tggaaatcgg attcggccgt
     1861 cgagccgtcg ccgatccatg ccgcggcaat gcgcgtgtcg cccttgatgg ccgatgccat
     1921 ggcccagccg acagcctgcg ggtattgcgt gccaagattg ccggaaagcg aaaagaagcc
     1981 gtgcgctttc gagctgtaga gcactggcag ctgccggccc ttcagtggat cacggctgtt
     2041 ggaatagatc tggcacatca tatcgaccag cgggtaaccg gcagtgatca gcagcccctg
     2101 ctggcggtag gtcgggaagt tcatgtcccc cttttggagc accgtctgaa aggcgcaggc
     2161 aatggcctcc tcgccggtgc actgcatata gaaggacgtc ttgccctgcc gctgggccat
     2221 gagcatccgc tggtcgtaga cgcggacttt catcatggtc ttgagcccgg ccagcagctc
     2281 atcgtcgctc agcgtgccag cccatggccc gacggcctcg ccggcccggt tgagcacgcg
     2341 gatgattgag taagcgaggt cccggatctc ttccggaggc acgtccacgg gaggtctgcg
     2401 cacgctgccg gccttcggaa tgggaacgcc gctgaagtca ggcttgctgc cggggcgaaa
     2461 ctccggctcg ggaacgtgca agcggagcgg ggcgcgaacg gccatgtgag tctgccttcc
     2521 gtgcgggatg ggctgtgggc aatcgcccgt gcagcccatg cccgagtgcc tcaactgcaa
     2581 aagctagggc aattcgccca gaaggttctt ttgaagttgc tgccgattga cggcacagca
     2641 gaagatttat ctgtaataat cgcgatacgg cgaatttcgt tccatgcgag tccttgcatg
     2701 accaagttag caccgacccg agccacgatg aagcggctcg acgaaatcga tcgcgcgatc
     2761 ctccgggagc tgcagaagaa cggccgcctc tcgaacgccg agctcgctga gcgagtcgga
     2821 ctgtcatcga gtccgtgctg gcagcgcgtg aggcggctcg aggagagcgg ctacatcgag
     2881 ggctatgctg cggtcctcag ccagaagcag ctcggtatgc cggaaaccgt gatcctcgag
     2941 gtcagcctcg agcgtcatga tgacgaaatc ctggagaagt tcggtcgagc gctggcagac
     3001 ttccccgaag tgctcgaggc ctacctgacc accggcgaat acgattactt cgtcaagatc
     3061 gcgatcgacg gcacggaggg gtacgaacgg tttctgcgag agcgcctcta ccggatcccc
     3121 ggaattcgtc agacgcgatc aagctttgcg ctgaggtgcc tcaagcgcca gatttcggtg
     3181 acaccttagc tggcatcccc catccggtcc gatcgcctga atgctgggcg gtctccagtg
     3241 gcacgggccg ggccgataat gttggccgga aatgagcgag acgccagtcc gaaagattat
     3301 tcatatcgac atggatgcgt tctacgcgtc ggtcgagcag cgggacgatc cgacacttcg
     3361 cggtcggccg gtggcggtcg gtcacgcggg gaagaggggt gtcgttgccg cggcaagcta
     3421 cgaagcccgc aagttcggtg tgcgttcggc catgccctcg acaacggcgc tacggaagtg
     3481 ccccgagctg gtgttcgtgc caccgcgctt cgaggtctat cgggccgtat ccgagcagat
     3541 caggcgcatt ttcgcgga
//
LOCUS       DASVDU010000334         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_1429335, whole genome shotgun sequence.
ACCESSION   DASVDU010000334 DASVDU010000000
VERSION     DASVDU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429335"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="VF226_22210"
     CDS             <1..893
                     /locus_tag="VF226_22210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX7076763.1"
                     /translation="YVHGAGVDEPLVRYEGVSVSAANRRYLHANHQGSIVALTDASGT
                     MLRVNAYDPYGVTGASNTGRFQYTGQAAIPELGLYYYKARFYNPSLGRFMQTDPIGYE
                     DDVNLYAYVKNDPMNHADPTGTCLDENCPGAVFTPAESRAIMDSPLGGFLPGAGLVQA
                     ADAISAGNYGMAALGVAGELGGKYAKAAGELLGKLGKAFGDEIAKSVTSFLKKNPVPT
                     KTGAIDAKALPSDGVAVQATKAGDVPGSKAVYEKQIDSAGTTVQSTKTTYDAEGNIVH
                     VKDKLTNETYAPSCTNRQCP"
     gene            907..1257
                     /locus_tag="VF226_22215"
     CDS             907..1257
                     /locus_tag="VF226_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076764.1"
                     /translation="MLKHLAHRGALLELLQFERPFPELRSSILAFPYDYDGDPVVLTR
                     NHLAHVLQCVVDGTISEQELEAWSELIEGRPGIEYESGMESRLSEILFQLSTPEINEP
                     VTVEKCWRLLKDLR"
     gene            complement(1574..1846)
                     /locus_tag="VF226_22220"
     CDS             complement(1574..1846)
                     /locus_tag="VF226_22220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076765.1"
                     /translation="MAAEAGISHEALNAAIEAHARERRTAHEGPRFVSRRRSRSGPSV
                     LDLLVPGPWPLTAKGVALAGVAGIGIVGLALAFLAVAQMALLSSCS"
     gene            1820..>2830
                     /locus_tag="VF226_22225"
     CDS             1820..>2830
                     /locus_tag="VF226_22225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076766.1"
                     /translation="MRDAGFRRHLLQLFHRVGAGQRAFIYNDFGQLIEQTDPPGADGV
                     RRKTYITYETSTGISRRSVVRVCGDTTTCGTPDEIRTEYEYWGSTYLPSVVRRIDAAH
                     GEVLETNCEYDLAGRLLVEDGPLPGTGDAKYFRYDEYGRKFWDIGPADDTGVRLARRF
                     AYREADDKLKSVEEGLVPSATSTALTVFRRTDHVYDSRRHPTRESVSANGTTHTVLQR
                     AFDLQGRLTCEARRMNPAAFGSLRASACTLGPEGSYGRDRITHNVYDAAGQLLQIQRA
                     YGTPLQQDYATYTYTPNGQRQTVKDANDNLSTFEYDGFDRLLKLRFPVATKGANQSSS
                     TDY"
BASE COUNT          596 a          855 c          873 g          506 t
ORIGIN      
        1 gctacgtgca cggcgccgga gttgacgagc cgctggtccg gtacgaaggc gtttcggtct
       61 ctgccgccaa ccggcggtac ctccacgcca accaccaggg ctcgatcgtc gccctcacgg
      121 acgcgtcggg cacaatgctc cgggtgaacg cgtacgaccc ctacggcgtc accggcgcga
      181 gcaacaccgg gcgcttccag tacaccggtc aggcggccat cccggaactc gggctctatt
      241 actacaaggc gcgcttctac aatcccagcc tgggccggtt catgcagacc gaccccatcg
      301 ggtatgaaga tgacgtaaat ctctatgcat acgtcaagaa tgatccgatg aatcacgccg
      361 atccaaccgg tacctgtctc gacgagaatt gccccggcgc cgtttttaca ccagctgaaa
      421 gccgagcgat tatggactca ccgctgggtg gattcctccc gggggcggga ttagtccaag
      481 ccgcggatgc tatttccgcc ggaaactacg gaatggccgc gttaggagtg gccggagaac
      541 tcggcggaaa gtatgctaaa gctgccggag agctcctagg gaagttgggt aaagcgttcg
      601 gcgatgaaat tgcgaagagc gtcacgagtt tcctgaagaa aaatccggtt ccgactaaaa
      661 caggcgcgat cgatgccaag gccctaccta gtgacggcgt ggcagttcag gcgacgaagg
      721 caggcgacgt gccaggatcc aaggcggttt acgaaaagca gattgactca gcgggcacta
      781 cggttcagag cacgaaaacg acatacgacg ccgagggcaa cattgtccac gttaaggaca
      841 aattgacgaa cgagacgtat gcaccatcat gcacaaaccg ccaatgtcct tagtcggtgc
      901 tgactcatgc taaagcactt ggcacatcgc ggtgcacttc tggagttgct gcagttcgag
      961 cgaccctttc cagagttgcg gagttcaatt cttgcctttc cgtatgacta tgatggcgat
     1021 ccggttgtgt tgacgaggaa ccatctcgcg catgtgttgc aatgcgtggt cgatggcacg
     1081 atatccgagc aggagctaga agcgtggtca gaattgatcg aaggccgtcc tggtattgag
     1141 tacgaaagtg gaatggagtc gcgcttgagc gagatactct tccagctttc gacccccgaa
     1201 atcaatgagc cggtcactgt cgaaaaatgc tggcgcttac tcaaggacct tcgttagctg
     1261 gcaagagcga tcttcatgtt cttatgttct tcataaagcg gcgcgatcat aagccgtcac
     1321 gttggctgtt ggtctatcga aacacgtgtt aggtaaagaa aatttcgatg aggcgctcaa
     1381 ggtattaacg ccgtactaag gcgctctaga ccggaaattg ggattgaacc gctggcccaa
     1441 agagcggagt agaggggcag tagcgagcgt tggccgacgg gtcgctgttc acgtttgggg
     1501 ggccgtcagt cgaccaggtt ttcatcggcg cgtgctggtt agaacggcga agcgccgatt
     1561 gcgatcagca gcgtcacgag caggaggaaa gcagcgccat ctgcgcaacg gccaggaacg
     1621 cgagcgcgag accgacgatg ccgatgccgg cgacgccggc gagcgcgacg cccttcgccg
     1681 ttaggggcca tgggccgggc acgagcaggt cgagcaccga cggcccgctt cgcgaccgtc
     1741 tccgggagac gaaccgcggt ccctcgtgcg ccgtccgccg ttcgcgggcg tgcgcctcga
     1801 tcgcggcatt gagcgcctcg tgcgagatgc cggcttccgc cgccatctcc tccagctctt
     1861 ccatcgagta ggagccggcc agcgcgcatt tatctacaat gatttcggcc agctcattga
     1921 gcagaccgac ccgcccggtg cggacggtgt ccggcgcaag acctatatca cctacgagac
     1981 gagcacggga atcagccgcc ggagcgtcgt gcgggtctgc ggcgacacga cgacgtgcgg
     2041 cactcccgac gagatccgta ccgaatacga atactggggc agcacctacc tgccctcggt
     2101 cgtgcgccgg atcgatgccg cgcatggcga ggtgcttgag acaaattgcg agtacgacct
     2161 tgccggccgg ctgctggtcg aggacgggcc cctgcccggt accggcgacg cgaagtattt
     2221 ccgctacgac gaatacggcc gcaagttttg ggacattggc ccggcagatg acaccggtgt
     2281 gcggctcgcc cggcggttcg cctatcgcga agccgacgac aagctgaagt ccgtcgaaga
     2341 gggtttggtg ccgagtgcga ctagcactgc gctcacggtt ttccgccgca ctgaccacgt
     2401 atacgacagt cggcgccatc cgacccgcga atccgtgtcg gcgaacggga cgacccacac
     2461 tgtcctgcag cgcgcgttcg acctgcaggg gcggctcacc tgcgaggccc gccggatgaa
     2521 cccggccgcc ttcggctcgc tccgcgcaag cgcgtgcacg ctgggaccgg agggaagcta
     2581 cggtcgcgat cgcatcaccc acaacgtcta cgacgcggcg ggccagctgc tccagatcca
     2641 gcgcgcctac ggcactccct tgcagcagga ctacgctacc tacacctata cgccgaacgg
     2701 ccagcgccag acggtcaagg acgcgaacga caacctgagc accttcgaat acgacggctt
     2761 cgatcgcctg ttgaagctgc gctttccggt ggccacgaag ggtgccaacc agtcgagcag
     2821 caccgattac
//
LOCUS       DASVDU010000335         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_232112, whole genome shotgun sequence.
ACCESSION   DASVDU010000335 DASVDU010000000
VERSION     DASVDU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232112"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF226_22230"
     CDS             <1..398
                     /locus_tag="VF226_22230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076767.1"
                     /translation="LQSLPSRRKEVETDKEGNQLRWIHDRAALKARFKKLSDGEENRL
                     IANAKKGAELHERRSAANEYDVGGADAWAFGNTAFRIFYGRELTTIDRFKLYRRPPPP
                     PLRREKPALSPMIEPIAFSSTPLIQSTRG"
     gene            404..1216
                     /locus_tag="VF226_22235"
     CDS             404..1216
                     /locus_tag="VF226_22235"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076768.1"
                     /translation="MVETLDLPKAQVVIEATVVEVQLNDTLESGVEFFLQYKGLVAGS
                     GIPVGNQSPSSGGMIGIGADIGSVSVDAILRALREVTNLRVVSSPYLTVVDGQSARLV
                     IGDQIPFAATSQTSNNQGAVTVTQQVEILDTGIVLEITPRIHANNSVTLDIVQSVSSP
                     VGEVEAGNLTPTIATRDISSKILAQSGRTILLGGLIQDRNELREEGVPGMSKLPVVGP
                     LLRRNKNGAERTELLILITPRVVRASGEIEAITRILRGAQLYQAPRDLKSPH"
     gene            complement(1237..1392)
                     /locus_tag="VF226_22240"
                     /pseudo
     CDS             complement(1237..1392)
                     /locus_tag="VF226_22240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1589..2158
                     /locus_tag="VF226_22245"
     CDS             1589..2158
                     /locus_tag="VF226_22245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7076769.1"
                     /translation="MYSGLGLVLLVLLLVIAGYAIFVYNDLVKARQIMREAWSGIDVQ
                     LKRRSDLIPNLIETVKGYAQHERAALGEVTALRTRAQSLRSDDVVGRAQVEGMLGQAL
                     GRLFAVAEAYPDLKANENFIQLQNSLEALESEIQMSRRYYNGAARELNIKVESFPSSL
                     VANMFGFRQAEYFEIENAEDRAVPRVSFN"
     gene            2162..>2742
                     /locus_tag="VF226_22250"
     CDS             2162..>2742
                     /locus_tag="VF226_22250"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX7076770.1"
                     /translation="MLTPAARIVIAFLLAFSFARSAAAAEEIRNFHVLIEVGADGTLA
                     VTETIEINVEGRQILRGIYRDIPLRYEDANGRTREVGLDVQSVTRDGNSEPYAIERSS
                     GVLRIRIGNANVFLPHGVHTYRIAYETTRQIRYFEDHDELYWNVTGNGWDFPILRASA
                     EVRLPSGARATDVTYFTGPYGSTEQAARVTRLDG"
BASE COUNT          565 a          814 c          822 g          541 t
ORIGIN      
        1 agcttcagag cctgccttcc cgccgcaagg aagtggagac ggacaaggaa ggcaaccagc
       61 ttcggtggat ccatgatcgc gcggcattga aagccaggtt caagaagctg agcgatggcg
      121 aagaaaaccg cctgatcgcc aatgccaaaa agggtgccga actgcacgag aggcgctccg
      181 ctgcgaacga atatgatgtg ggaggcgcgg atgcctgggc tttcgggaac accgcgttcc
      241 ggattttcta tggtcgtgag ttgacgacca tcgaccgttt taagctctac aggcgtccgc
      301 ctcctccgcc cctcaggcgg gagaaacccg cattgtcgcc gatgatagaa ccaatagcat
      361 tctcgtctac tccacttatt cagtctacaa gaggatgagg gaggtggtgg agacgctcga
      421 cctgccgaaa gcccaggtcg tcatcgaggc gacagtggtg gaggttcagc tcaatgacac
      481 tctggaatcg ggtgtggagt tcttcctaca atacaagggc ctggttgctg gttccggcat
      541 tcccgtcggt aaccaatccc cctccagtgg aggcatgatc ggcatcggcg cggatatcgg
      601 cagtgtgagc gtggacgcca ttctgcgggc gctgcgtgag gtgacgaatt tgagggtggt
      661 gtcctcgccc tatctcacgg ttgtcgatgg ccagtccgcc cgtcttgtaa tcggcgatca
      721 aatccccttc gcggcaacct cccagacctc gaacaatcag ggggcggtta cggtcacgca
      781 gcaggtcgag atcctggaca ccggcatcgt gctcgagatc acgcctcgca tccatgccaa
      841 caactcggtc accctggaca ttgtccagtc ggtatcctcc ccagtcggag aggttgaagc
      901 cggaaacctg acgccaacga tcgccacgcg cgacatctca tcgaaaatcc ttgctcagtc
      961 cgggcgcacc atccttctcg gcgggctgat acaagaccgc aacgaattgc gggaagaggg
     1021 tgttccgggg atgtcgaaat tgcctgtcgt tggtcctctg ctccggcgga acaagaacgg
     1081 cgccgagcgc actgagctcc tgatcctgat tacgccgcgg gtagtacgcg cctcaggcga
     1141 gatcgaggcc ataacgcgca ttttgcgggg tgctcaactc taccaggctc ctcgcgatct
     1201 caagtcgcct cactagcgcg cgttgcccct accaaagatt ggcgctctcg tgcccaccgc
     1261 tcgtcgccgt gttcggcgct tgcccaccgt caacccttcc tcccaggaga gccgatagat
     1321 gcgattaatc cctgacggct cgtgctcccg ccgcagcagg atgaacaacc gtcgatagcc
     1381 gaagcgtcgg cgcaaaaaag acagtgcgtg tgaaggattg accgcgctgc gtatgaagga
     1441 ttgacggcac ttcgccatcc ttgacgctca aattggtgca aactcggtca agagaacttg
     1501 ctaaccgtcg gcttttggtg gacttttgct catctgatgg cccggccagg tgcgaggtac
     1561 ggcatcggga ctgcctcaag gagaaatcat gtattccggt cttggccttg tcctccttgt
     1621 gctccttctg gtcatcgccg gctatgcgat ctttgtctat aacgacctgg taaaggcccg
     1681 gcagatcatg cgcgaggcct ggtccgggat cgacgtgcag ctcaaacgcc gctccgacct
     1741 catccccaat ttgatcgaga cagtgaaggg ctatgcgcag catgaacgcg cagcgctggg
     1801 ggaggtaacg gcgctgcgca cccgcgcaca aagtctgcgg agcgacgatg tcgtcggccg
     1861 ggctcaggtg gaaggcatgc tggggcaggc gctgggccgg ctttttgccg tagcggaagc
     1921 ctatcctgac ctcaaggcca acgagaattt catacagctg cagaactcgc tcgaggcact
     1981 cgaaagcgaa atccagatgt cgcgccgcta ctacaatggc gcggcgcgcg agctgaacat
     2041 caaggtcgag agctttcctt ccagcctggt cgccaacatg ttcggctttc gccaggcaga
     2101 gtatttcgag atcgagaatg ccgaagaccg cgcggtgccc agagtctcgt tcaattgacg
     2161 catgctgacg ccggccgcac gcattgtcat tgcattcctg ctcgccttca gttttgccag
     2221 aagcgcggct gcggctgaag aaatccgcaa cttccacgtg ctgatcgagg tcggagcgga
     2281 tggcacgctg gcagtgaccg aaaccatcga gatcaatgtc gagggtcggc agattttgcg
     2341 cgggatctac cgcgatattc cgctgcgtta cgaggatgcg aacggccgta cgcgcgaggt
     2401 ggggctggat gtgcaatcag tcacgcggga cggcaattcg gagccttatg cgatcgaacg
     2461 cagttcgggc gtgctgcgca tccgcatcgg caatgcaaat gtcttcctgc cgcatggcgt
     2521 gcacacctat cggatcgcat acgagacgac gcgccagatc cgctatttcg aggatcacga
     2581 cgagctgtac tggaacgtca ccggcaatgg ctgggacttc cccatcctga gagcgagcgc
     2641 cgaggtcaga ttgccttccg gtgcccgtgc aaccgacgtc acctacttta cagggcccta
     2701 cggatcgacc gaacaggcgg cccgggtcac acgcctcgac gg
//
LOCUS       DASVDU010000336        11336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_774876, whole genome shotgun sequence.
ACCESSION   DASVDU010000336 DASVDU010000000
VERSION     DASVDU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11336
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774876"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1996)
                     /gene="recJ"
                     /locus_tag="VF226_22255"
     CDS             complement(161..1996)
                     /gene="recJ"
                     /locus_tag="VF226_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7076771.1"
                     /translation="MGALARPKPELFDGRRSAFLGVTRSVRGLQWRERIDPADLNLAT
                     AISQRHDLPELLGRVLAARGVRLDEVPVVLDPTIKALMPDPSVLQDMDKAAARLADAI
                     IAREPIAVFGDYDVDGAASVALIHRFLAHHGLGCRVYIPDRLFEGYGPNVAAIEGLIK
                     DGARLIVTVDCGTASFEPLGRAREMGSDVIVIDHHQADERLPDVHALVNPNRQDDLSG
                     LGQLCAAGVTFMVLVATARELRRRDFYSAASPAPDLLGLLDLVALATVCDVVPLTGLN
                     RAYVVKGLQVMRTRRNIGLTALFDAAGLAQAPTPYHLGFVLGPRINAGGRIGDAALGA
                     RLLSIDDEAEAARIAQLLDKLNKERKAIEAEMLEQAMAEAERQLEADPTQPIIVLGSE
                     SFHKGVVGLVASRLTERFQRPSCVISWENETGTASLRSVAGVDIGAAVRAAVSKGHLV
                     KGGGHAMAAGLTVERNRLDRLRAFFCETLAVAATHARATAGLDIDGAMTSGGATDELL
                     QLIERAGPYGQGNPQPRFAFPAHRIKYAKVVGERHIRCAVEAGNGSRIDAVAFRAADQ
                     PLGEALLSTGGMPIHLAGTLRRETWGGRDRIELTIDDAADPRRQG"
     gene            complement(2108..3121)
                     /locus_tag="VF226_22260"
     CDS             complement(2108..3121)
                     /locus_tag="VF226_22260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX7076772.1"
                     /translation="MIRLSVLDQSVAIRGRPHRDSISETVELAELCEGLGYHRFWVSE
                     HHSHDTIVGTAPEILMAAIAARTRSIRVGSAGVMLPHYSAFKVAEQFRLLEALAPGRI
                     DLGVGRAPGSDSRTAQALNPFGTMRTLQFPEQVRDLIAWVSGEDLPEVHPFRQVRAYP
                     AGDTAPVVWMLGSSDYGAQVAAHFGLPYSFAYFITDGVGVEQALSLYRQLYRPSPRHP
                     EPVANICVWALVAETEAEAQRLFTSRAHWRVARDRGLLAPIDAPEASLPFEPTPLEEA
                     RIAEMRRTAFVGTPADVGRRLRTLADELGLEEIVILTWSHDPAARRRSYELLAREFEL
                     KAR"
     gene            3286..3432
                     /locus_tag="VF226_22265"
     CDS             3286..3432
                     /locus_tag="VF226_22265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7076773.1"
                     /translation="MANRLGWFLGLLVLLILILFWVIPWNRIETSVTPQESSAPYEPP
                     RRID"
     gene            complement(3429..5042)
                     /gene="cimA"
                     /locus_tag="VF226_22270"
     CDS             complement(3429..5042)
                     /gene="cimA"
                     /locus_tag="VF226_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX7076774.1"
                     /translation="MSRERLYLFDTTLRDGAQTTGVDFSLQDKRRIAELLDGLGIDYV
                     EGGYPGANATDTAFFASRPQLKTSRFTAFGMTKRAGRSVSNDPGFQATLQSGADAICL
                     VAKAWDYHVRVALAISNEENLEAVAQSVQAIREAGTEAMIDCEHFFDGYKGNRAYALA
                     VARTAYEAGARWVVLCDTNGGTLPHEIERIVRDVAEVIPGTHLGIHTHNDTENAVANT
                     LAAVRAGVRQIQGTINGIGERCGNANLTSIIPTLLLKSEFADRFETGVTPERLMRITQ
                     ASRILDEILNRAPNRHAPYVGESAFATKAGIHASAILKEPETYEHVKPESVGNRRRVL
                     VSDQAGKSNVIAELERLGIAMPKNDERIGRLLEEVKEREAAGYAYEGAEASFELLARR
                     MLGEVPKYFTVDSFRVMVEKRHNAIGRLITVSEATVRVFVSGSDEPVWSVAEGNGPVH
                     ALDQALRKDLGRYQKYINDIELVDYKVRILTGGTEAVTRVLIESHDKATGERWFTVGV
                     SPNIVDASFEALVDSINYKLLKDGAEAAT"
     gene            complement(5179..5574)
                     /locus_tag="VF226_22275"
     CDS             complement(5179..5574)
                     /locus_tag="VF226_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7076775.1"
                     /translation="MNAPTATPALDHIGFTVSDFARAKAFYSAALQPLGITIQLEFTE
                     HGGVAGFGRSKPEFWIAAGDSPTSPFHIAFQAANRAEVDAFYKAAIAAGAKDNGAPGL
                     RPQYHPNYYAAFVFDADGHNIEAVCHHPE"
     gene            complement(5901..7292)
                     /gene="cysS"
                     /locus_tag="VF226_22280"
     CDS             complement(5901..7292)
                     /gene="cysS"
                     /locus_tag="VF226_22280"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX7076776.1"
                     /translation="MTLTLYNTLTRRKEPFQPLDPKNVRMYVCGPTVYDYAHIGNARP
                     VIVFDVLFRLLQHIYGPEHVTYARNITDVDDKINARAAEEGVSIRELTERTTRQFHED
                     IAALGVLPPTVEPRATDHIEDMKRLIERLVAKGCAYVAQDHVLFDVEAMPDYGRLSNR
                     SLEEMEAGARVEVAPYKKGPMDFVLWKPSKPGEPAWPSPCGISVPGRPGWHIECSAMA
                     ERHLGEVFDIHGGGIDLVFPHHENELAQSRCAHGTPIMAQVWMHNGFLMVEGEKMSKS
                     LGNFVTIHELLATDKFGGRRWPGEVLRFNMLRTHYRQPIDWTVEGLEQSLAILDSWFP
                     FATEFDEADQNKASPVLEALCDDLNTPKAIAELHRLSDAGERGALGFALALLGFDGQS
                     LKSEREANEARRRELAKTVEPRIAERLAARKAKNFAEADRIRDELAAMGVQLKDSKDP
                     VTGEIVTTWEVKR"
     gene            7641..8189
                     /locus_tag="VF226_22285"
     CDS             7641..8189
                     /locus_tag="VF226_22285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEX7076777.1"
                     /translation="MALKDEIAAARARWNVLTLYQKFEELVIVILTGLIAVVVVFAVW
                     NLALKIALSLLAASFDPTDHAEFQAVFGMILTIIIALEFKRSILVVAERRYSVVQARA
                     IVLIALLALVRKILILDLNTTSAGQLLGLGAAILALGAVYWLVGEQERRDRAAMRSMV
                     AAAAPSEGLEPAVSDARSPDRR"
     gene            8326..9297
                     /locus_tag="VF226_22290"
     CDS             8326..9297
                     /locus_tag="VF226_22290"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEX7076778.1"
                     /translation="MSKRRAGGFACAVALLMAAAGPVLAQGWPWDWGWGQQEPRRPRE
                     PVFREPPPQEQPSWQSSNQSAICLQLEQRLAQEANRGSQSRELLPKLEQEMRAADRAY
                     QSGQAQLERNNCYEYFLFAKSLRRTRLCVDLNRQVEQARDRLAEIEAQRQQILSSSNR
                     SYQEDIIQELARNRCGATYEQQARRFNPFSSIWQDEDSGGPFGGRYESLPFATFRTVC
                     VRLCDGYYFPVSFSTLPNHFDHDAEVCQSKCAAPAELFYHQNPGGSMEDAVSYSTRRP
                     YASLPVAFRYRKEYVQGCSCKQSEYDPRVDQPQKRAEGTTGQPARTR"
     gene            9378..9947
                     /locus_tag="VF226_22295"
     CDS             9378..9947
                     /locus_tag="VF226_22295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7076779.1"
                     /translation="MTDDIATAAEIRPIGPDDLAEVRSLQASSFRVLAAQVFTEEEVA
                     AFSDHVYSLAYTEALSEAIRRGQLFGAWFDRQLVATAGWTAGDGSISSARIRWVHVRP
                     MFTGIGLGTRLVETAEAAAQKAGFDVLSVEATLNAAEFFAGLGYETSSHGVRSLPLGQ
                     SLAVAYMRKQLSRRPPQLTSLSEDAAEEA"
     gene            complement(9950..10201)
                     /locus_tag="VF226_22300"
     CDS             complement(9950..10201)
                     /locus_tag="VF226_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX7076780.1"
                     /translation="MSMTTTAQLTSSGLTTPLAPPRRSLFAWVRTMADAYRQSFERRA
                     AVARLRDLDDHLLKDIGLDRSEIVSMVYGQPDATRRDRT"
     gene            10442..10518
                     /locus_tag="VF226_22305"
     tRNA            10442..10518
                     /locus_tag="VF226_22305"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10476..10478,aa:Asp,seq:gtc)
     gene            10765..>11336
                     /locus_tag="VF226_22310"
     CDS             10765..>11336
                     /locus_tag="VF226_22310"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7076781.1"
                     /translation="MTESTGFHFLDGGGEVAIAIRHLNWDSNPLGPIEDWPSSLKTIV
                     SLALNSAFPMWIVWGPELTSIYNDAFRPILGDKPTALGKCISDVWPEIWDTIGPLVKR
                     TFAGTPTFLEDMLLTINRKGEPEEAYFTFCFSSLRDEAGEVQGMLATVYETTRKVKAE
                     RQAELLIRELSHRGKNMFAIVAAIARQTFQS"
BASE COUNT         1966 a         3494 c         3773 g         2103 t
ORIGIN      
        1 attgaaaatc tggcgtccta accgatagac gaagggagcg ctgggcgcag ggtatagggg
       61 ggtttcaggg ggctggcaag ggagcaggtg gcgtttccgc gccgcacctc ccggtgtgcc
      121 ggccgcgcgg aaactgcccg aaaggcgcgc cggcactcga tcatccctgc cgtcgtgggt
      181 cggccgcatc gtcgatggtc agctcgatgc ggtcgcgacc gccccaggtc tcgcgtcgga
      241 gcgtgcctgc cagatgaatc ggcatgccgc cggtggagag cagggcttcg ccgagcggct
      301 ggtcggcagc gcggaaggcg accgcatcga tgcgggaacc attgccggct tcgaccgcgc
      361 agcggatgtg acgctcgccg accaccttgg cgtacttgat gcggtgggcg gggaaggcga
      421 agcggggctg cgggttgccc tggccgtacg gtcctgcgcg ctcgataagc tgcagcaact
      481 cgtccgtggc gccgcccgaa gtcatggccc catcgatatc caggcctgcg gtggcgcggg
      541 cgtgggtggc ggccacggcg agtgtctcgc agaagaatgc gcgcagccga tcgagccggt
      601 tccgctccac cgtcagtccg gcagccatcg catggccacc gcccttgacg agatggccct
      661 tcgagacggc ggcacgcacg gccgctccga tatcgacgcc cgccacggag cgcaaggagg
      721 ctgtgccggt ctcgttttcc catgagatca cgcacgaggg ccgctggaag cgctcggtca
      781 ggcggcttgc gaccaagccg acgacgccct tgtggaagga ttcggagccg agcacgataa
      841 tcggctgcgt ggggtccgct tcgagctgcc gctcggcttc cgccatggcc tgctcgagca
      901 tctccgcttc gatcgccttg cgctccttgt tgagcttgtc gagcagttgc gcgatgcgcg
      961 ccgcttccgc ctcgtcgtcg atggacagca ggcgcgcgcc gagcgccgca tctccgatgc
     1021 gccctccggc attgatgcgc gggccgagga cgaagcccag atggtatggc gtcggggcct
     1081 gggcgaggcc cgcggcgtcg aacagggccg tcagtccgat gttgcggcgc gtacgcatca
     1141 cctgcaggcc cttgacgacg taggcacggt tgaggccggt aagcgggacg acgtcgcaca
     1201 cggtcgccaa ggcgacgagg tcgaggaggc cgagcaggtc gggcgcgggg ctcgcggccg
     1261 agtagaagtc gcggcggcgc agctcacgtg ccgtcgcaac caggaccatg aatgtcactc
     1321 cggctgcgca cagctggccg agacccgaca ggtcgtcctg ccggttcgga ttgacgaggg
     1381 catgaacgtc tggaagccgt tcgtcggcct ggtggtggtc gatgacgatc acgtcgctgc
     1441 ccatttcgcg ggcacgcccc agtggttcga agctggcggt gccgcaatcc accgtcacga
     1501 tcagccgggc gccgtccttg atgagcccct cgatggctgc cacgttggga ccgtagcctt
     1561 cgaacagccg atccggaatg tagacccggc agccgagccc atgatgcgcc aggaagcggt
     1621 gtatcagggc aacggatgct gctccgtcga cgtcgtaatc gccgaatacg gcgatgggtt
     1681 cacgagcgat gatggcatcg gcgagacgag cggctgcctt gtccatgtcc tgcagcacgc
     1741 tcgggtcggg catgagcgcc ttgatcgtcg ggtcaaggac gaccgggacc tcgtcgagcc
     1801 tgacaccacg ggcggccaga acgcggccga gcagctccgg cagatcgtgt cgctggctga
     1861 tcgcggtggc gaggttgagg tcggccgggt cgatgcgctc tcgccattgc aatccgcgta
     1921 cggatcgcgt gacgccgaga aacgcggacc gcctcccatc gaacagctca ggcttcggtc
     1981 tcgcgagtgc ccccatcccg tacccctgcg aatcaggccc gcagtgttgc acgcggtcag
     2041 ggcaggttgg agagatgcgt ccgaagctcc tgtggacatc tgcacaggcc tgcgtgcaga
     2101 cgcccgttca gcgcgctttc agctcgaact ctcgggcaag cagctcatac gatcgccggc
     2161 gggcggcggg atcgtgggac caggtcagga tcacgatctc ttcgagtccg agctcatcgg
     2221 caagcgtgcg gagacgtctg ccgacgtctg ccggagtgcc gacgaacgcg gtgcgccgca
     2281 tttcggcgat acgagcctct tcgagagggg tcggctcgaa tgggagggac gcttccggag
     2341 catcgatagg ggcgagcagg ccccggtccc gggccacccg ccaatgggcc cgcgacgtga
     2401 agagccgctg cgcttccgct tcggtctccg caaccagcgc ccacacgcag atgttggcga
     2461 caggctcggg atgccggggg cttggccggt agagctggcg gtagagcgac agcgcctgct
     2521 cgaccccgac gccatcggtg atgaagtagg cgaacgaata gggcagcccg aagtgtgccg
     2581 cgacctgggc gccgtaatcc gagctgccga gcatccagac cacgggcgcg gtgtcgccgg
     2641 ccggataggc gcggacctgt cgaaaggggt gaacctcggg caggtcctcg ccgctcaccc
     2701 aagcgatgag gtcgcggacc tgttccggga actgcagcgt gcgcatggtg ccgaacgggt
     2761 tgagcgcctg cgcggtacgg ctgtcagagc ccggtgcgcg gccgacgccg agatcgatgc
     2821 gtccgggcgc cagcgcctcc agcaagcgaa actgctcggc caccttgaac gcggagtaat
     2881 gtggcagcat cacgccggca ctgccgactc tgatcgagcg cgtgcgtgct gcaatggccg
     2941 ccatcaggat ttcgggggcc gtgccgacga tcgtgtcgtg gctgtgatgc tcggagaccc
     3001 agaatcgatg atagccaagt ccctcgcata gctcggccag ctccaccgtc tcgctgatcg
     3061 agtcgcggtg cggccggcct cgaatggcga cggattggtc gagcactgag agccggatca
     3121 tgagagccca tcacctttgc agcaggcaaa ctgcggacag attagtccta acgtgctccc
     3181 tgggcgagac gatcgggaat gatgcatcgc cgcgcgacct cgcctgaggg aacggactag
     3241 gcgcaagtga gttgttcgcc gaacgcatcg gcttggaatg tttccatggc caatcgactg
     3301 ggctggtttc tcgggctcct cgtcctgctg atcctgatcc tgttctgggt gatcccatgg
     3361 aaccggatcg agacctccgt gacgccacag gagtcgagcg cgccgtatga gccgccgcgg
     3421 cggatcgact aggtcgccgc ctccgccccg tccttcagca gcttgtagtt gatcgagtcg
     3481 acaagggcct cgaagctcgc atcaacgatg ttgggcgaga cgcccacggt gaaccagcgc
     3541 tcgccggtgg ccttgtcgtg gctctcgatc agcacgcggg tgaccgcctc ggtgccgccg
     3601 gtcagaatgc ggaccttgta atcgacgagc tcgatatcgt tgatgtactt ctgatagcgt
     3661 ccgagatcct tgcgcagcgc ctggtcgagc gcgtgcaccg ggccgttgcc ctcagcgacc
     3721 gaccacaccg gctcgtcgct gcctgagacg aagactcgga ccgttgcttc cgagacggtg
     3781 atgagccggc cgatggcgtt gtggcgcttt tcgaccatga cgcggaagct gtcgacggta
     3841 aagtatttcg gcacctcgcc gagcatccgc cgggccagca gctcgaatga ggcctcggcg
     3901 ccctcgtagg catagccggc ggcctcgcgc tccttcacct cctcgagcag acggccgatg
     3961 cgctcgtcgt tcttcggcat cgcgatgcca agccgttcca gctcggcaat gacgtttgat
     4021 ttgccggcct gatcggagac gagcacacga cggcggttgc cgacgctttc cggcttcacg
     4081 tgctcgtagg tctccggttc cttcaggatc gcagacgcat ggattccggc cttcgtggcg
     4141 aatgcggact ctccgacgta aggcgcatgc cggttgggcg cgcggttgag gatttcgtcg
     4201 aggatccggc ttgcctgcgt gatccgcatc aggcgctcgg gcgtcacgcc cgtttcgaag
     4261 cgatcggcga actcggattt cagcaacagc gtcggaatga tcgaggtcag gttggcattt
     4321 ccacagcgct cgccgatgcc gttgatcgtg ccctggatct ggcgcacgcc ggcccgcacg
     4381 gcggcgaggg tattggcgac ggcgttctcg gtatcgttat gcgtgtgaat gccgagatgc
     4441 gtgccgggga tgacctcggc cacatcgcgc acgatgcgtt cgatctcgtg cggcagtgtt
     4501 ccgccgttcg tgtcgcagag caccacccag cgggcgccgg cttcgtaagc ggtgcgcgcg
     4561 acggcgagcg cataggcgcg attgcctttg tagccgtcga aaaaatgctc gcaatcgatc
     4621 atcgcctcgg taccggcttc gcgaatggcc tgcaccgact gggcgaccgc ttccagattt
     4681 tcctcattgc tgatggcgag cgcgacccgc acgtggtaat cccaggcctt ggcgacgaga
     4741 cagatcgcgt cggctcccga ctgaagcgtc gcctgaaagc ctggatcatt ggaaaccgac
     4801 cggccggccc gcttcgtcat gccgaatgcg gtgaagcgtg aggtcttcaa ctgcgggcgc
     4861 gaagcgaaaa aggcggtgtc cgtggcgttg gcgccgggat agccgccctc cacgtagtcg
     4921 atcccgagcc cgtcgagcag ctcggcaatc cgccgcttgt cctgaagcga gaaatccacc
     4981 ccggtggtct gcgcgccatc gcgcaaagtg gtgtcgaaaa ggtataggcg ttcgcgagac
     5041 aagtgctggt cctcagatga cttcggcgag acggagggcg agggcgacag ccgcgatgat
     5101 cacggcgatt ttcaaaatgc gataatagat ccggtggcgc tgaaacggcg gcgtgtcatt
     5161 cgagtcggcc atgggtgctc actcggggtg atggcaaacg gcttcgatgt tgtgcccgtc
     5221 cgcatcgaaa acgaacgcgg cataatagtt ggggtgatac tgcggccgca gccctggtgc
     5281 gccattgtct ttcgcacccg ccgcgatggc agctttgtaa aaggcatcga cctcagctct
     5341 gttggccgct tggaaagcta tgtggaatgg gcttgtcggg ctgtcgccgg cggcaatcca
     5401 gaactccggt ttgctgcggc cgaacccggc gacgccgccg tgttcggtga actccaattg
     5461 aattgtgatt ccgagcggct gcagtgcggc cgaatagaat gctttggcgc gcgcaaagtc
     5521 gctgacggtg aagccgatgt ggtcgagggc gggggtggcg gtcggagcgt tcatatcatc
     5581 tccttttcaa ctggccgctg cgaatgtcgt ggctgacgat tgtgtagtcc ctacgcgttt
     5641 cgcaaaagga tttcttcgca aatcacgatg acccggagcc gaccggaatc tccctctccc
     5701 aaagggagag ggtcggggtg aggggttatg aggtaagacg tgatacgaga cggaggaatt
     5761 cgcggcggcg aatttgcaac cggttcggaa atccggcaag cggcgttgcc ccctcaccct
     5821 aaccctctcc cccacgggga gaggggactg gctggtggcc tggcttcgta gaatgagtag
     5881 aatgagatcg agaggctgca ttatcgcttc acctcccacg tcgtgacgat ttcgccggtg
     5941 acggggtcct tggagtcctt cagttgaacg cccatcgctg cgagctcgtc gcggatgcgg
     6001 tcggcttcgg cgaagttttt ggctttccgg gcggccagac gctcggcgat gcgtggctcg
     6061 acggttttgg ccagctctcg acgcctcgct tcgtttgctt cgcgctccga tttcaacgac
     6121 tggccatcaa atccgagcag cgccagcgcg aagccgagtg cgccgcgttc gccagcatcg
     6181 ctcaggcgat gaagctcagc aatggccttc ggcgtgttga gatcgtcgca taaagcttcg
     6241 aggacgggcg acgccttgtt ttgatcggcc tcgtcgaatt ccgttgcgaa aggaaaccaa
     6301 gaatccagaa tggccaggct ctgctccagg ccctccaccg tccagtcgat cggctggcgg
     6361 taatgcgttc gcagcatgtt gaaccgcagc acttcgccag gccaacgccg accaccgaat
     6421 ttgtccgtcg ccagcagctc gtgaatcgtc acgaaattcc ccaggctctt ggacatcttc
     6481 tcgccctcga ccatgaggaa gccgttatgc atccagacct gcgccatgat cggcgtgccg
     6541 tgggcgcagc gggactgggc gagctcgttc tcatgatgcg ggaaaacgag atcgatgccg
     6601 ccgccgtgga tgtcgaaaac ctcgcccagg tgccgctctg ccatcgccga gcactcgatg
     6661 tgccagccgg gccgaccggg gaccgaaatc ccgcaggggc tcggccaagc aggctcgccg
     6721 ggctttgacg gtttccatag gacaaaatcc atcggcccct tcttgtaggg tgcgacttcc
     6781 acgcgagcgc ccgcctccat ctcctcgagg ctgcggttgg agagccggcc gtagtcgggc
     6841 atcgcctcga cgtcgaacag cacgtggtcc tgggcgacgt aggcgcaacc tttggcgacc
     6901 aaccgctcga tcagccgctt catgtcctcg atgtgatcgg tggcccgcgg ctcgaccgtc
     6961 ggcggcagca cgccgagagc ggcgatgtcc tcgtggaact gccgcgtcgt acgctcggtc
     7021 agctcgcgaa tcgagacccc ttcctcggcc gcccgcgcgt tgatcttgtc gtcgacgtcg
     7081 gtgatgttgc gcgcgtaggt gacgtgctcc gggccgtaga tatgctgcag caggcggaac
     7141 agcacgtcga aaacgatcac cgggcgcgcg ttaccgatgt gggcgtagtc gtagacggtc
     7201 ggcccgcaca cgtacatgcg cacgttcttc ggatcgagcg gctggaacgg ctccttgcgg
     7261 cgggtcagcg tgttgtaaag cgtcagcgtc acgttcgtcc cctggcctgc tggcggggcg
     7321 tggctctatg aattgggaga aggtgagcgg ccgcgcgcca gcgatgagct agctcgaaat
     7381 aatgcagcag atgatgccgc agcagtccgg tgcggcagag atcgtcgcgg ctcttttcat
     7441 ggcgtcctta tgcgtgagcc cggtggggga cgtcaagcgg cgtagtcgac tggcccgggc
     7501 ctgggcatcc ggccttgtgg cgcatctggt cgggccgcgg ccaactaccc ggaaactcgg
     7561 agggctatgc cccggtttga ccatatatca agcaggacac ccgttcgcag ctgttccacg
     7621 actccatgat ggaggcagag atggcgctca aggacgagat tgctgccgcg cgggcgagat
     7681 ggaacgtcct gacgctttac cagaagttcg aggagctggt catcgtcatc ctgaccggcc
     7741 ttatcgcggt cgtcgtcgtg tttgccgttt ggaatctcgc actgaagatc gccctcagtc
     7801 tgctcgcggc ctcgttcgat ccgaccgatc acgccgagtt ccaagccgtg tttgggatga
     7861 tccttacgat catcatcgcg ctggagttca agagatcgat tctggtcgtc gccgaaaggc
     7921 gctacagcgt cgtccaagcg cgcgccatcg ttctgatcgc gctcctggcg ctggtgcgca
     7981 agatcctgat ccttgacctc aacacgacga gtgcggggca gctcctcggg ctgggtgccg
     8041 ctatactggc gctcggggct gtctattggc tcgtcggaga acaggagcgc cgtgatcgtg
     8101 ccgcgatgcg atcgatggta gccgcggccg caccgagcga ggggctggag cctgcagtat
     8161 ccgatgcgag gtcgccggac cgccgttgat cgagaggtgg acgccccatt tgcgctgcat
     8221 tgaccggcta gcggggatcc ttcaagaatg cgggagactc agcgcgggga acccaggctg
     8281 cgctgcatgg gtgtgaatgt cacgatgcgc ctcgttccag ctggaatgtc gaagcgtcgc
     8341 gccggcggtt tcgcctgcgc ggttgcattg ttgatggccg ccgccggacc cgtcctggca
     8401 caaggctggc catgggactg gggctggggg caacaagagc cgcggcggcc gcgtgagccc
     8461 gttttccgcg agccgcctcc tcaggagcag ccatcctggc aatcgagcaa ccagtcggcg
     8521 atctgcctgc agctcgagca gcgactggcg caggaggcga accgcggatc acaatcacgc
     8581 gagctgctgc cgaagctgga gcaggagatg cgcgctgccg atcgggccta tcagtccggt
     8641 caggcgcagc tggagcggaa caactgctac gagtatttcc tgttcgcaaa atcactgcgg
     8701 cgtacgcgcc tgtgcgtgga cttgaacagg caggtggagc aggcgcggga ccggctggcc
     8761 gagatcgaag cgcagcgaca gcagatcctc tcgtcgagca accgttccta tcaggaggac
     8821 atcatccagg agctggcgcg gaaccgctgc ggtgccactt atgagcagca ggcgcgccgc
     8881 ttcaatccat tctcgtcgat ctggcaggac gaggacagcg gcgggccgtt cggcggccgc
     8941 tacgagagcc tgccgttcgc aacgttccgc accgtctgcg tccggctgtg cgacggttat
     9001 tactttccgg tgagcttctc gacgctgccc aaccacttcg atcacgacgc ggaggtgtgc
     9061 cagtcgaagt gcgccgcgcc ggcggaactg ttctatcacc agaaccccgg cggcagcatg
     9121 gaggacgcgg tgtcctacag cacccgtcgg ccctacgcgt cgcttccggt ggcgttccgc
     9181 taccgcaagg agtacgtcca gggctgctcg tgcaagcaga gcgagtacga tcccagggtg
     9241 gaccagcctc agaagcgggc agaggggacg acaggacagc ctgcgcgcac cagatgaggc
     9301 atcttgacca gtcgggcgga tcgtgaccta gtcgggcggg cggggggcaa agtgaccgca
     9361 atgccgggac gcatcctgtg acggacgata tcgccactgc cgctgagatt cgacccatcg
     9421 gcccggacga tctcgccgag gtgcgctcgc tgcaagccag ctcatttcgg gtgctggcag
     9481 cccaagtctt caccgaagaa gaagtcgcag ccttttcgga ccatgtctac agcctggcct
     9541 ataccgaggc cttgtccgag gcgatccggc gtggccagct cttcggggcg tggttcgacc
     9601 gacagctcgt cgcgacggca ggctggacgg cgggggacgg cagtatctcc tcggcgcgga
     9661 tccgctgggt tcacgtaagg cccatgttca ccggcatcgg gctcgggacg aggctcgtcg
     9721 agaccgccga agccgccgcg caaaaggccg gcttcgatgt gctgtcagtc gaggcgacgc
     9781 tgaacgccgc cgagttcttc gccgggctcg gttacgagac gtcctcacat ggcgtgcgct
     9841 cactgcctct tggtcagagc ctggcggtgg cctatatgcg caaacaactg agccgtcggc
     9901 cgccgcagct cacatccttg agcgaggatg ctgccgagga ggcctagcct cacgtgcggt
     9961 cgcgccgggt ggcatcgggc tggccataaa ccattgagac gatctcagac cggtcgaggc
    10021 caatgtcctt caggaggtga tcgtcgagat cgcgcagcct tgcaacggca gccctgcgct
    10081 cgaacgattg cctgtaggca tcggccattg tcctcaccca tgcgaaaagg ctgcggcgcg
    10141 ggggtgccag cggggtggtc aggccagacg atgtcagctg tgccgttgtc gtcatgctca
    10201 tttgatatct ccgacattct ttcttggggc cttgggacga cgcggcaaac ttcggctgcc
    10261 gctcacgtcc tgctgttcta ggctggctgt tccaatccgg ctgttcccca cggaacatgc
    10321 cgctggcggt gaatgaccag gaagctgctt gcgccgcgcc gcatcggctt gacgtacccc
    10381 ggcgtctcac gtaaaggatc gccgtgacgg cgggtctgcg cggctcgcca tatttttctt
    10441 agcgggcgta gctcagttgg ttagagcgcc ggcctgtcac gccggaggcc gcgggttcaa
    10501 gtcccgtcgc tcgcgccatt ggtttcaatg gcttacagtt tctctgaacg agttataaga
    10561 gcccctggcc ggcgcctaaa gctcttggcc ggttttgccc ccgcagcttg gcccttccac
    10621 gcttccgcgc ttcgtccgca cccacaccgc cgcgagctga actggcgcgt aagccgtctc
    10681 ttacgacgtt ttggggtcaa ccgaacccta agaccgcctt gagccttctg tgtgcaagac
    10741 gagttatgaa cacagggtgc cgaaatgact gaaagcacag ggttccactt cctcgatggt
    10801 ggaggagaag tggcgatcgc tattcggcac ctcaattggg actccaatcc attgggtccg
    10861 atcgaagact ggccctcaag tctcaagacc atcgtgagct tggcgctgaa ctcagcgttt
    10921 ccgatgtgga tcgtatgggg gccggagctg actagcatct acaatgacgc ttttcgccca
    10981 atacttggcg acaagcccac agccctcggc aagtgcattt ccgacgtatg gccagagatc
    11041 tgggatacaa tagggccgtt ggtgaagcgg acgttcgccg gcacaccgac tttccttgaa
    11101 gacatgctgc taacaatcaa tcgaaaaggt gaaccggaag aggcttactt caccttctgc
    11161 ttcagctcgc tacgggatga ggccggtgaa gtgcaaggta tgctcgccac cgtgtacgaa
    11221 accactcgga aagtgaaggc ggagcggcaa gcggagctgc tcattcgcga gctttctcac
    11281 cgcggcaaga atatgtttgc gatcgtggcg gccattgcgc ggcagacttt ccagtc
//
LOCUS       DASVDU010000337         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_864941, whole genome shotgun sequence.
ACCESSION   DASVDU010000337 DASVDU010000000
VERSION     DASVDU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864941"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VF226_22315"
     CDS             <1..1449
                     /locus_tag="VF226_22315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7076782.1"
                     /translation="PRVNWMFCTEPDPNLNNRRGYWPRGKVLGGSSSINAMVFVRGHP
                     ADFDAWAADGNPGWGWNDVLPYFNRMESFSGGADEWRGTGGPLHVTDVSSDLHPICES
                     FVTAGREAGLPVCNDFNGASMEGVGPYQITMRGGVRMSTARAYLRPAMKRPNLRVETG
                     AHVARILIEGRRAVGVSFTKGGKIHEARAGREVVLAAGAIHSPLLLQASGIGPPGVLR
                     SVGLEIVHGSPQVGQNLQDHLCIDYLYRSRVPTLNQDLGTWKGRIKAALQYAVRRRGP
                     LSLSVNQAGGFFRSRPELDRPNIQLYFSPLSYLRLPPGRRALMSPDPFPGFLLSAQPC
                     HPASRGYVQLRSTDPSAAPVIVPNSLSDERDLDELVDGARFLRKLATTPALSRIIENE
                     LVPGSRVQSREEFIEDIRERASSVFHPVGTCRMGPDPATSVVDSRLRAHGLDALRVID
                     ASIFPTLTSGNTNAPTIMVGEKGADLLLEDAV"
     gene            1446..2786
                     /locus_tag="VF226_22320"
     CDS             1446..2786
                     /locus_tag="VF226_22320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7076783.1"
                     /translation="MNAAEISRVNGTRPVPNRLFTDNCKLTPYWWDEVPRPQPEEKPL
                     PRSVDVAVIGSGYTGLNAALQVARGGRNTIVLDAEDAGWGCSTRNGGQVSTSIKPSYD
                     RLARRYGAQRAFDILKEGQNSLAWLGKFIKDEKMDCDFAVVGRFHAAHNKAQYESLAR
                     RIENQPKGLEVEAHVVPRSEQHTELGTDIYYGGVVYCQHASVNPARYHQELLRRVQQA
                     GAGIFARCPVTAIEKDNGRFRLSTPRGVIEARDVVVATNGYTGALTPWLRRRVIPIGS
                     YMIATEPLPPELVARLMPRGRIVSDTRKVVYYYRASPDRRRILFGGRVSLNETDPRLS
                     GPKLHADMVQIFPELAQTRISHSWCGFIAYTFDELMHVGRHDGVYYAMGYCGAGVGTA
                     SYFGMRIGQQVLGLPEGRTALDGLSFETRPFYTGNPWFLSASVLYYRWRDRLPV"
     gene            complement(2893..>3158)
                     /locus_tag="VF226_22325"
     CDS             complement(2893..>3158)
                     /locus_tag="VF226_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX7076784.1"
                     /translation="LTIYVIDVAQGEKIPRKGGPGITRSDLLVINKTDLAPHVGADLA
                     VMERDTRQARGSKPYVFANVRSGEGVGAIARFIEQQGGLAARS"
BASE COUNT          534 a         1025 c         1020 g          579 t
ORIGIN      
        1 ccccgggtca actggatgtt ttgcaccgag ccggatccca atctgaacaa ccgccgcgga
       61 tattggccgc gcgggaaagt actcggcggt tccagctcca ttaatgccat ggtcttcgtc
      121 cgcggccatc cggccgactt cgacgcctgg gcggctgatg ggaatcccgg gtggggttgg
      181 aatgacgtgc tcccctattt caatcgcatg gagagcttca gcggtggcgc cgacgaatgg
      241 cgcggcaccg gcgggccgct gcatgtcacc gatgtctcga gcgacctgca cccgatatgc
      301 gagtcattcg tgacggcggg cagggaagcc gggctgcccg tctgtaatga tttcaatggc
      361 gcctcgatgg agggtgtcgg cccctatcag atcaccatgc gcggtggcgt gcggatgtcg
      421 accgctcgtg cgtatctgcg gccagcgatg aagcggccga atttgcgcgt cgagaccggc
      481 gcccacgtgg cgcgcattct gatcgaaggc aggcgggctg tcggtgtgag cttcacgaag
      541 ggcggaaaaa ttcatgaggc ccgagccggg cgggaggtcg ttctggcggc cggcgctatc
      601 cattcgccgc tgctgctgca ggcatccggg attggacctc ccggtgtgct gcgctccgtc
      661 gggctcgaga tcgtgcacgg cagcccgcag gtcgggcaga acctgcagga tcacctctgc
      721 atcgattatc tctatcggtc acgggtcccc acactcaacc aagacctggg gacgtggaag
      781 gggagaatta aagccgcgct acaatatgcc gtgcgccgcc gcggaccgct tagccttagc
      841 gtgaaccagg cgggtggctt cttccgctct cgcccggaat tggatcggcc caacattcag
      901 ctctatttct cgccgctcag ctatctccgg ctcccgccgg gccgcagggc gctgatgagc
      961 ccggatccgt ttcccggctt tttgctgagc gctcagccct gccatcccgc aagccgcggg
     1021 tatgtccagc tccgctcgac cgacccatct gccgcgcccg tcatcgtccc caattcgcta
     1081 tccgatgaac gcgacctgga tgagttggtg gacggcgcca ggttcctgcg aaagctggcg
     1141 acgacgcccg ccctgtcgcg catcatcgag aacgagctcg ttccgggcag ccgtgtgcaa
     1201 tcccgtgagg agttcatcga ggacatccgc gagcgcgcct caagcgtgtt ccatccagtc
     1261 ggcacgtgcc gtatggggcc ggaccccgcg acgtccgtgg tcgacagccg cctgcgcgcc
     1321 cacgggttgg atgcgctccg cgtgatcgat gcttccatct ttcccaccct cacgtccggc
     1381 aacacgaacg ctcccaccat catggtcggt gagaagggag cggatctttt gctcgaggac
     1441 gcggtatgaa tgctgccgag atttcgcggg tcaacggaac acgaccggtg ccgaacaggc
     1501 tttttacaga caactgcaag ctgaccccgt actggtggga tgaggtgccg cgcccgcagc
     1561 ctgaggagaa gccgctgccg cgctccgtgg atgttgccgt catcggctcc ggctacacag
     1621 gactgaatgc agccttgcag gtggcgcgcg gcggtcgcaa caccatcgtg ctcgacgcgg
     1681 aggatgccgg ctgggggtgc agcacgcgca acggtggcca ggtctcgacc agcatcaagc
     1741 cgagctacga tcggctggcc cgccgatacg gcgcccagcg ggccttcgac atcctgaagg
     1801 agggccagaa ctcgctggcc tggctcggca agttcatcaa agacgaaaag atggactgcg
     1861 atttcgcggt cgttgggcgt ttccacgctg cacacaacaa ggcccaatac gagtctttgg
     1921 cgcgccggat cgaaaatcag ccgaaggggc tcgaggtcga agcgcacgtc gtgccgcgct
     1981 ccgagcagca cacggagctg ggcacagata tctactacgg cggcgtcgtc tattgtcagc
     2041 acgcgtccgt caacccggcc cgctatcatc aggagctgtt gcgtcgcgtc cagcaggccg
     2101 gcgcgggaat cttcgcgcgc tgtcctgtca cggccatcga aaaggataat ggccgtttca
     2161 ggctaagcac cccgcgcggc gtcatcgagg cccgcgacgt cgtggtggcg accaacggct
     2221 acacgggtgc cctcacgccc tggctgcggc gaagagtgat tccgatcggc agctatatga
     2281 tcgcaaccga accgctgccc ccggagttgg tggcgcggct catgccgcgg gggaggatcg
     2341 tcagcgacac gcgaaaggtc gtctactatt accgtgcctc gcccgatcgg cgccgcatcc
     2401 tgttcggcgg gcgcgtttcc ctcaatgaaa ccgacccccg cttaagcggg ccaaagctcc
     2461 acgccgatat ggtccagatc ttccccgagc tcgcgcagac gcgcatcagt cactcatggt
     2521 gcgggtttat cgcctacacc ttcgacgagc tgatgcacgt cggccggcac gatggcgtgt
     2581 actatgccat gggctattgc ggcgccggcg tcggcacggc gagctacttc ggcatgcgca
     2641 tcggccagca ggtcctcggg ctgcccgaag gtcgcaccgc gctcgacggc ctctcgttcg
     2701 aaacacggcc attctacacc ggcaatccct ggttcctgtc ggcgtcggtg ctgtattacc
     2761 gctggcggga ccggctccct gtgtgatgcg ccgcgaccgc gcctcatctc agggcgcagc
     2821 tttgaagatc cgggctggca ccagcagatc cagccattca tgacgcgcat ccggattgac
     2881 cgggcaaggt cttcatgacc ttgccgccag gccgccttgt tgctcgatga accgggcgat
     2941 cgcacccacg ccttcgccgg agcggacgtt cgcgaaaacg taaggcttgc tgccgcgcgc
     3001 ctgccgcgtg tcgcgctcca tgacggcgag gtcggcgccg acgtgcggtg caaggtcggt
     3061 tttgttgatg acgagcaggt cggagcgggt gatgccgggc ccgcctttgc gcgggatctt
     3121 ctcgccctgg gccacgtcga tgacgtagat ggtgaggt
//
LOCUS       DASVDU010000338         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U2041
            k141_191834, whole genome shotgun sequence.
ACCESSION   DASVDU010000338 DASVDU010000000
VERSION     DASVDU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961364
            Sequence Read Archive: SRR25158368
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 20:56:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,467
            CDSs (total)                      :: 4,423
            Genes (coding)                    :: 4,386
            CDSs (with protein)               :: 4,386
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 37
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 8 of 37
            Pseudo Genes (incomplete)         :: 35 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191834"
                     /isolate="SMAG_U2041"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.66305556 N 120.4575 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1266
                     /locus_tag="VF226_22330"
     CDS             406..1266
                     /locus_tag="VF226_22330"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX7076785.1"
                     /translation="MPTIRRFAKLLAIATFVGLSAAWVYWAVDGLTLSDADAYRAAAN
                     RLLAGEDLYIVPRTQGDAFRYAPWFALIWIPLAALPEWAANTVWLAALAMASTLAVVP
                     LARAPSVWLRLTALLGGSVLIWTAARGNVHPLVVLALVWGIDRRTGPIWIALTASLKA
                     VPILFALVYVARREWRRAFVAVAVTVLLVAPMPFFGWDFESTEAGPSLSLYYLVSPAG
                     WVIGAGAALLAATVSAMQGWPATRLAAATAAIAVLPRLLLYDATYLLPGLTPYPTSRP
                     EAATPQRTAS"
     gene            1263..>2520
                     /locus_tag="VF226_22335"
     CDS             1263..>2520
                     /locus_tag="VF226_22335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7076786.1"
                     /translation="MIATPRPTWTHRRAEIALLLVPALTLGAIARFVPIANVSFPLND
                     GGLFYVMSQDLVRLGFALPDVTSYNNAGIPFAYPPLALYALGILSAAGLDAIQLLRWI
                     PFVVSVMTIPLVYLVAKELLGSWRIAGVAALAFALIPRSYEWTITGGGLTRGLGMAMA
                     LLAIWQGLRLIRTPNPMHVALTGLFGGLTVLTHPEASLFVVITLGTFVAAFGRTIRTA
                     MAMVIAGIIGGLVSAPWWLSILLLHGPGLLVGAADSRTAQLPNALRNFLFGDFTGASA
                     LDIFLGIGFVGLLVQVGRGRYVLPVWLLGLVIFIVAAGFTFAAVPWAMLTAIAVMEVV
                     LPSAGRLFPAHVHARAIVTGGLLGAGMLATLATGYSTATPIHGLSPEQRSAMQWINES
                     LPPDARFVVVTGVDWPKDATSEWFPAL"
BASE COUNT          378 a          778 c          761 g          603 t
ORIGIN      
        1 cgacgtcatc gccatcgaac gcgtcttctt cttcttcggc aacgtgccct cagaagtcct
       61 tcagcgggca ttgcatacgg ccggacagat caacgtcgtc gaccttgtga aggcgtcaat
      121 ctgattcggc aattgcaggc actcacatcc ggagagtggg tccggtcatc tctccaacga
      181 ggcacgcatg tccatcacat cgcagccaat tactactcca ttgtggtaag gaggtggcac
      241 tggcgcctcg acaacgagtc attgctgcgc accgttattg tctttctgct cgtggcgata
      301 ttcgaaggca ccgtcgcccc gagctgattt cgacgatcgt ggcaagttcc gaatgggacg
      361 ttcgttccga tgaggccgtg tgccgggctg ctacgatccc gcgcgatgcc gaccatccgg
      421 cgcttcgcga agctgctggc gatcgccacc ttcgtcggcc tgtcggcggc gtgggtgtac
      481 tgggctgtcg acgggctgac gctgtcggac gccgacgctt atcgagcggc cgctaatcgg
      541 ttgctcgcgg gggaggacct gtacatcgtg ccgcgcactc aaggtgacgc attcaggtat
      601 gcaccttggt tcgcgctcat ttggataccg ttggccgcgc ttcctgagtg ggcggcgaac
      661 actgtgtggc tcgctgcgct tgccatggcg agcactcttg ccgtcgtacc gttggcacgg
      721 gctccatcag tctggctgcg attaactgct ttgctcggag gctcggtcct gatctggacc
      781 gcggcccgtg gaaatgtcca tcccttagtt gtgctcgcgc tcgtttgggg gatcgaccgt
      841 cgaacaggtc ccatctggat tgccttaacc gcctcgctga aggccgtccc aatcctcttc
      901 gcgctggtct acgtcgctcg acgggaatgg cgacgagcgt ttgtcgcggt tgcggtgacc
      961 gtgttgctgg tggccccgat gccgtttttc ggttgggact ttgagagcac tgaagctggc
     1021 ccgagcctga gtctttacta cctcgtctcg ccagcaggtt gggtgattgg agctggagct
     1081 gcactgctcg ccgcaacggt gtctgcgatg caggggtggc ccgcaactcg gcttgcagcc
     1141 gccacggcag ccatagcggt cttgccacgc cttctcttgt acgacgccac gtaccttctt
     1201 cctggactca cgccgtatcc caccagccgg ccggaggcag caactcctca gcgaaccgcc
     1261 tcatgatcgc gacgccgcga cccacctgga ctcataggcg ggctgaaatc gccctgctcc
     1321 tcgttccggc tctgaccctc ggggcgatcg ctcgcttcgt gccaatcgcg aatgtttcat
     1381 tcccgctcaa cgacggcggg cttttctatg tgatgagcca agatctcgtc cgtttagggt
     1441 tcgcgttgcc cgatgttacg agctacaaca acgccggcat tcccttcgcc tatccgccgc
     1501 tcgcgctcta cgccctgggc atcttgtcag ccgctgggct tgatgccatt cagttgttgc
     1561 ggtggatccc tttcgtcgta agcgtcatga cgatcccgct cgtttatctc gtggcgaagg
     1621 agctactcgg atcgtggcgc attgccgggg tcgccgcctt ggccttcgct ctcatcccgc
     1681 ggagttacga atggactatc acggggggcg gcctgacgcg aggacttggc atggccatgg
     1741 ccctgcttgc gatatggcag ggtctccggc tcatccggac gccgaacccg atgcatgtcg
     1801 ccctcactgg gctttttggg ggcctgacag tgctaaccca tccagaagca tccctcttcg
     1861 tcgtgattac gcttggaaca ttcgtcgcgg cctttggtcg gaccatccgt acggcgatgg
     1921 caatggtgat tgcagggatc atcggcgggc tggtatcagc gccttggtgg ctttcgatcc
     1981 tcctcctgca cggacctggc ttgctggttg gtgcggctga ctcgaggacc gctcagctgc
     2041 ccaatgctct tcgaaatttt ctcttcggcg atttcaccgg cgcctcagca ttggacatat
     2101 tcctgggcat tggcttcgtt gggcttctcg tccaggttgg ccgcggtcga tacgtcttac
     2161 ctgtttggct actcggtctc gtcatattca tcgttgccgc ggggttcaca ttcgcagcgg
     2221 tgccttgggc gatgctgacg gccatagccg tcatggaggt agtgcttccg tctgctggtc
     2281 gcttgtttcc ggcgcacgtt cacgcgcgcg cgatcgtcac gggcggtctc ctcggggcgg
     2341 gcatgctggc cactttggcg actggttaca gcacggcgac tcctattcat gggttgagcc
     2401 cggaacaacg ttcagcgatg cagtggatca acgagtcctt gcctcctgac gcacggtttg
     2461 tcgtcgttac aggagtggac tggccaaaag atgcgacgtc ggaatggttt cccgccctcg
//
